LOCUS       DAWBMW010000001        38732 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_336_length_38732_cov_2.879774, whole genome shotgun sequence.
ACCESSION   DAWBMW010000001 DAWBMW010000000
VERSION     DAWBMW010000001.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 38732)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 38732)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..38732
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_336_length_38732_cov_2.879774"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4114..4123
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    22871..22975
                     /estimated_length=105
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    28625..28634
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    30201..30210
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         8177 a        10483 c        11478 g         8459 t
ORIGIN      
        1 agtctagaga ggttgatatg cgccaaaccg aagccctggc cattctgaac gccggcgcga
       61 acgtgttcct gaccggtgcg cccggtgccg gtaagaccta tgtcttgaac gagttcatcc
      121 gccaggctcg ggccgacggc gccagtgtag cagtcaccgc gtccacaggc atcgcctcca
      181 cgcatatcaa cggccaaacc attcattcgt ggagcggcgt cggcgtggcc accgccctga
      241 gcgccagcct gctcaaactc atcaaatccc ggcgcaaacg caagattcag gccacggaca
      301 tcctcgtcat tgacgaggtt tccatgctgc acgcttggct gttcgacatg gtcgaccaag
      361 tctgccgcga agtacgccgt gatccacgcc cgttcggtgg catccaggtt gtcttgtccg
      421 gcgacttctt ccagctgccg ccggtttcgg tgtccgggcg caaccgcgac ctcatcgccc
      481 ccacgcccga attcatcgcc agtcgcgaac ggtatgcccg tgccggcttg aaccccgaag
      541 gcttcatcac cgaatcgctg gtatggcccg aactcaaccc ggtgatctgc tatctgaccg
      601 aacagcaccg tcaggatacc ggcgaactgc tgaccgtgct caccgacatt cgcgaaggcg
      661 acgtgaccca gtccgaccgt gacgtgctcg tcactcgtct gggcaaactg cccgagcccg
      721 gccaggtcgc cgtccacctg ttcccggtga accggcaggc cgacaatctc aacgacatgc
      781 gcctgaacca gatcatgctt gatccacacg agttccacgc cgagacagca ggccctgcca
      841 acctggtcga ccgactcaaa aagaacatgt tggcccccga gcgattgata ctcaaggaag
      901 gcgcggctgt catggccctg cgcaacgaca ccgatcgcca gtacgtcaac ggctcgctcg
      961 gcaccgtgcg tggcttcgca caggagaaca agggcggctg gccgatcgtg gaattcgaaa
     1021 acggcaacat cgtcaccatg aaacaggcca catgggagat gatggacggc gacaccgtgc
     1081 tcgcctccgt cttccaggtg ccgttgcgtt gcgcgtgggg catcaccatc cacaagtcgc
     1141 aaggcatgac gcttgaccgt gcggtcatgg atctgaagcg caccttcgcg ccgggcatgg
     1201 gatatgtggc gttgtcccgc gtcgaatcgc tgggcggact gtatttggct ggcgtcaacg
     1261 aacgcatgtt cctcgtctcg cccgatgccg tggtgctcga cggcgacctg cgcgaagcct
     1321 cggcctcggc ttccgaccag ttggcggatt aaggttcaaa cgcgttcaaa cccgccgagc
     1381 cggacgaatt cggtgccaat cccgacgatg acttcgccca ggacgcgttg ttctgacatg
     1441 attcatgttg tgtcgctaag aggatttgcg ctatactgat acagctaatt ctcagtaaga
     1501 gtggacctga tagccacttt gaatgctcag caggcaactg ctaggggcga gccatttggc
     1561 tcgccctgtt tcgtacagtt acaatgttca agacaatgat gtgcattgta gctacggtga
     1621 aaggtgtttt atgcggggga agcgttaccg gataggaatt gacgtcggtt tgaattctgt
     1681 gggccttgcc gcagtagaag tgtccgatga gaacagtcca gtgcgtctgt tgaatgcgca
     1741 aagcgtaatc cacgatggtg gtgtggatcc tcagaagaat aaggaagcga ttacgcgaaa
     1801 gaatatgtcg ggtgtcgctc gtcgcacgcg gcgtatgagg cgtcggaagc gtgagcgctt
     1861 gcacaagctc gatatgctcc tcggcaaatt tggatacccc gttattgagc cggagtcctt
     1921 ggataagccc tttgaagaat ggcatgtaag ggctgagctg gctacacgat acatcgaaga
     1981 tgatgaactt cgccgtgaga gtatttctat cgcattgaga catatggcaa ggcatcgcgg
     2041 atggcgcaat ccttatcgtc aggtggactc gctgatcagc gacaatccat attcgaagca
     2101 atatggcgaa ctgaaggaaa aagcgaaggc gtacaacgat gacgcaactg ctgcggaaga
     2161 agagagcact cccgcgcagt tagtggtggc gatgttggat gctggatatg cggaagctcc
     2221 tcgactgcgt tggcgtaccg gttctaaaaa accggatgcg gaaggctatt tgccggtacg
     2281 tttgatgcag gaagataatg ctaacgagct gaagcaaata ttcagggtcc agagagtgcc
     2341 cgcggatgag tggaagccgc tgttccgtag tgtgttttat gcggtgtctc ctaaaggttc
     2401 ggcagagcag cgtgtcggtc aggatcctct ggctcctgaa caggctaggg cattaaaggc
     2461 ttctttggct tttcaggagt atcgtatcgc gaatgtgatt acgaacctaa gaatcaagga
     2521 tgcttccgca gaacttcgca aacttactgt tgatgagaag caaagcattt atgaccaact
     2581 cgtgtcacct tcttcggaag atatcacatg gtctgattta tgcgatttcc tcggcttcaa
     2641 gcggagccag ttgaaaggcg tgggtagctt gaccgaggat ggagaagaac gtatatccag
     2701 ccgtccgcct cgattgactt cagtccagcg tatttacgag tcggacaaca agattcgtaa
     2761 accgttggtg gcctggtgga aatccgcgtc tgacaatgag catgaggcaa tgattcgcct
     2821 gctgtctaat acggtggata tcgataaggt gcgtgaagac gtggcgtatg cttctgcgat
     2881 tgaatttatc gatggattag atgatgatgc gttaaccaaa cttgactccg ttgatctccc
     2941 ctccggtcgt gctgcataca gtgttgaaac tttgcaaaaa ctcactcgtc agatgctgac
     3001 tactgatgat gatttgcacg aagcacgtaa aacgctgttc aacgttacgg atagttggcg
     3061 tccgccggca gatccaattg gggagcctct gggtaatcca tcggtcgacc gtgtgctgaa
     3121 aaacgtcaac cgatatctca tgaattgtca gcagcgctgg ggtaatcccg tctcagtgaa
     3181 cattgaacat gtgcgctctt cgttctcgtc ggttgccttt gcccgcaaag acaagcgtga
     3241 atatgaaaag aacaatgaga agcgttcgat attccgcagc tcgttgagtg aacagcttcg
     3301 cgcggatgag cagatggaaa aagtgcgtga atccgattta cggagattgg aagccattca
     3361 gcgtcaaaat ggacagtgcc tgtattgcgg ccgtacgatt acattccgta cgtgcgaaat
     3421 ggatcatatc gttccccgga aaggcgtcgg ctctacgaac acgaggacca atttcgctgc
     3481 ggtatgtgcc gaatgcaatc gtatgaaatc gaacactcca tttgcgattt gggcaaggag
     3541 cgaggatgcc caaactcgag gggtaagcct tgctgaagct aaaaagcgtg tgacaatgtt
     3601 tactttcaat cccaagtcat atgctcctcg ggaagtgaaa gcgttcaagc aagcggtaat
     3661 cgcaaggctt cagcaaacgg aagatgatgc tgccatcgat aatcgttcca tcgaatcagt
     3721 ggcttggatg gctgatgaac tccaccgccg tatcgattgg tatttcaatg cgaagcagta
     3781 tgtgaattct gcttcaatcg atgatgctga agccgagaca atgaaaacca cggtgtcggt
     3841 gttccaaggc cgtgtcacgg catctgcaag acgtgcagct ggcattgaag ggaagataca
     3901 ctttattggc cagcaatcaa agactcgtct ggatcgtcgt catcatgcgg tggacgcttc
     3961 cgttatcgcg atgatgaaca cggctgctgc gcagacgttg atggaacgtg aatcattgag
     4021 agaatcgcag cgtttgattg gtctcatgcc aggcgaacgt tcatggaagg aataccccta
     4081 tgagggcact tcacgctatg aatcgttcca tctnnnnnnn nnncttctac accggccttg
     4141 tggtgggctt tgacgcgttt gccggattat gatgaaaaag agggtttgcc ggaagattca
     4201 catcgtgaaa tacgagtgca tgatactcgg tattccgctg acgatgagat gggattcttc
     4261 gcgtcgcaag ctgcgcagat tgctgtgcag gaagggtctg cagatatcgg ttcggcgatt
     4321 catcatgccc gcgtgtaccg gtgctggaag acgaatgcca agggagttcg taaatatttc
     4381 tatggcatga ttcgcgtatt ccaaactgat ctgctacgcg cttgccatga tgatttattt
     4441 accgtgccgt tgcccccgca gtctatttcg atgcggtatg gcgagcctag ggtggtgcag
     4501 gcgctgcaaa gcggtaatgc tcagtacttg gggtcgttgg ttgtcggtga tgaaatcgag
     4561 atggattttt ccagtctcga tgttgatggt cagattggcg agtatttaca attcttctcg
     4621 cagtttagtg gagggaacct tgcatggaaa cattgggttg ttgatggatt ctttaaccag
     4681 acgcagttga ggataagacc tcgatacctt gctgcggaag gattggcaaa agcgttttct
     4741 gacgatgttg ttccagatgg ggtccagaaa attgtgacaa aacaaggatg gctacctccg
     4801 gttaatacag caagtaagac tgcggttcgt atcgtaagac gcaatgcttt tggcgaaccg
     4861 agattatcgt cggcacacca tatgccgtgc tcatggcagt ggaggcatga gtgaatcatg
     4921 caaaaccagt ggcgtgtaat cgactgtacc gatcttgacg gctccatctc ctatgaacgg
     4981 ggaaggatgc gagtcgtcaa gaacgataca ggggagtgcg tggatgtgcc acttgctcag
     5041 attgcggtag tgctactggg attgcatgtg cgatgttcgg ctgcggtatt acatgagatg
     5101 gcacggtatg gcgtgtcagt catgctctgc gattggcgtg gtgttcctga cgctgcgctg
     5161 catgcatgga ctgacatgcc taccacagtt accacgcggc aattggcgca gtcgaacatg
     5221 tctcttcctc gcaagaaaag tgcgtgggcg cgtattgtac agatgaagat tcgtggacaa
     5281 gctgcttgcc tcgattcatc aaaactcgaa ggtggaggct tgctacgagg tattgcagcc
     5341 tctgttcgtt cgggtgatcc ttccaattgc gaaggtcatg ctgcacgtga atattggagg
     5401 cgaatgtttc ctcaagatga gaaattcaga aggattccgg ggcagggtta tggtcggaat
     5461 gcgcagttgg attatgccta tatggtgctg cgaggattcg cggttaaagc ggtgatagca
     5521 gcaggattaa ttccatcttt aggcgtgaat catcacgggc gcggcaatta tttctgtctt
     5581 gcagatgatc tgttggaggt atataggccg gcagtggatt acagagtctc actgctgaat
     5641 gatgaagact cgttgcagga gaaggcagta aagaagcatt tggtagatgc tgtgaatcaa
     5701 caattcaacg gttccggttt gactataccg agctcattga atgattttgc gcagcaattt
     5761 ggtttatatt gcgaaggcaa aatcgacagg ttgcaggttc ccgaatatgt aggcgagtca
     5821 tgaaacgcga tgaggacagt ggcggcatgt ggtgcttggt gatgtttgat ttgccggtca
     5881 agacaaaacg gcaacggcat gctgcaactg tctttagaaa tatgttgctc gacatgggat
     5941 atggaatggt gcagtattcc gtatatgtgc ggtatacgcc tacgcaatcg ggtaataggg
     6001 cgacggtcaa aatgattaaa gacaatctgc cagctaatgg cttagttaga attttgcata
     6061 ttagcgatca tcagtggtcc acggcggccc gattttcttc tggaaaacgt gagattgagg
     6121 aagaaacgcc tgactttctc acgctttttt gaacgtagga gagtctgaaa agattgaaaa
     6181 atagcccttc ttgataggct atcaagctta tcaagaaggg tgaatgctaa ttcccagcca
     6241 cggtggatgg tacatgctta gcgcattcaa gcttatcaag aagggtgaat gctaattccc
     6301 agctggacga actgtctaac gatgcgatta tcaagcttat caagaagggt gaatgctaat
     6361 tcccagcgtg agcttgtccg tgtctttcat cagtggcaag cttatcaaga agggtgaatg
     6421 ctaattccca gctgtttacg ctcaaaaagc acattagatt caagcttatc aagaagggtg
     6481 aatgctaatt cccagcccga ggccggtagg gttgcgtgtc tggtcaagct tatcaagaag
     6541 ggtgaatgct aattcccagc gttgtaatcg gtcataatag ttttcaatca agcttatcaa
     6601 gaagggtgaa tgctaattcc cagctctccc agttgctgcc ccttggcgtc gacaagctta
     6661 tcaagaaggg tgaatgctaa ttcccagccg tattcatcgt ggcctcttcg cggtttcaag
     6721 cttatcaaga agggtgaatg ctaattccca gccgtacatg atggggtcct tgaccttgta
     6781 caagcttatc aagaagggtg aatgctaatt cccagcttgg tggtgttggg cgtccggcgc
     6841 agaacaagct tatcaagaag ggtgaatgct aattcccagc acagtattat tgtcctgata
     6901 ttcatgtcca agcttatcaa gaagggtgaa tgctaattcc cagctcgtcg tccatctctg
     6961 tacctcggtt tctcaagctt atcaagaagg gtgaatgcta attcccagct ccatgaccgc
     7021 gtccatcacc tcatgcccaa gcttatcaag aagggtgaat gctaattccc agccgttccc
     7081 cagtggggtg agtgtgatgc ccaagcttat caagaagggt gaatgctaat tcccagcacc
     7141 gattgcaacg cgaatatccc gggtatcaag cttatcaaga agggtgaatg ctaattccca
     7201 gcgttgttcc gggaacaact gggaacaact caagcttatc aagaagggtg aatgctaatt
     7261 cccagccttg cctccgttct gcatccagct gtcgcaagct tatcaagaag ggtgaatgct
     7321 aattcccagc acgcggcgaa gaatcttgtt atcgcggctc aagcttatca agaagggtga
     7381 atgctaattc ccagcgtcgc acgtggcatg gcatgttggc gaaccaagct tatcaagaag
     7441 ggtgaatgct aattcccagc tgttgagggg aaccgtatca aacctgattc aagcttatca
     7501 agaagggtga atgctaattc ccagctggtt tccgtgccgc attgatcgca gtacaagctt
     7561 atcaagaagg gtgaatgcta attcccagct catgggactg tgagcggcgg cggcgtgaca
     7621 agcttatcaa gaagggtgaa tgctaattcc cagctagaag tgcagattcc gttcctgcaa
     7681 accaagctta tcaagaaggg tgaatgctaa ttcccagcag gacgtgcaac gcaagtattt
     7741 caagatcaag cttatcaaga agggtgaatg ctaattccca gctcgtcgat gtgttccaag
     7801 aggtttggtg caagcttatc aagaagggtg aatgctaatt cccagctatt gttcgggtgc
     7861 gatggtgatg atcacaagct tatcaagaag ggtgaatgct aattttgaat gctggaatgc
     7921 ttgggttgat ttggctgatt tagctgtgat ggccgctgcg ggaatggccc ttgcgcggtt
     7981 ggcggcagag gttgcggcgc ggattgcggc actgattgtt gtgccaattg cgtctgcggt
     8041 gaaaaatatg gcgacggctg tgtctgactg tttatcggcg gatgttccgg ctcaggttgt
     8101 ggctggaatg gagaatattg cggttgcgcc attgtatgcc cctgcccctt cattgccatt
     8161 tcattggcgc atggcgaggg ctgaaaacca agatgatgaa tcctgcatga gttatccgtg
     8221 attcctgtgc gttggtacag cgcggaaggt tgagcagccg acatatgcag gcttgtcggt
     8281 tcacgcgaat atctttaaac ctatgacttc caataccctt cgtatgtcaa ccatgttcct
     8341 gcgcaccctg cgcgaggacc ccgctgatgc cgatgtcgat tcagccaagc tgctgcagcg
     8401 cgccggctat atccgcaagg ccgcacccgg tatctggacc tggctgccgc tcggtctgcg
     8461 cgtgctgaac aagatcgagg ccatcatccg cgaggagatc aacggcatcg gcgcccagga
     8521 agtgcacttc ccggccctgc tgccgcgcga accctacgag gccacccatc gctgggagga
     8581 gtacggcgat aacatcttcc gtttgaagga tcgccacgag gccgattacc tgctggcccc
     8641 cacccacgag gagatgttca cccttctggt caaggacatg tactcctctt acaaggacct
     8701 gcccgtcacc ctgtaccaga tccagaccaa gtaccgcgac gagttccgtc cgcgcgccgg
     8761 cctgatccgt ggtcgtgaat tcgtcatgaa ggacgcctac tccttcacca tcgatgaaga
     8821 aggcatgcgc aaggcctact atgacgagcg cggcgcttac gagcgtatct tccagcgtct
     8881 tgacctgaag tacgtacctg tgttcgccat gtccggcccg atgggcggct ccgcatccga
     8941 ggagttcctg gctccgatgc cgatcggcga agataccttc gcgctggcac cttccggcaa
     9001 ggcatggaat gtggaggccc tgagcactcc ggaactaccg gaaatcgatg cttccaccac
     9061 tcccgccgct tccaaggaag ccaccccgga tgccaagacc atcgataaca tgatcgagcg
     9121 tgccaacgcc gaccacccgc gtactgatgg ccgtgaatgg caggcttccg acatcctcaa
     9181 gaacgtggtg atcaccgtca agcaccctga ggatgaagag catgacgagc cgtggcgtga
     9241 agtgattgtc gtcggcgtgc ccggcgaccg tacggtggat atgaagcgac tggaagccca
     9301 gttcgcgccc gccgaacttg aggaagccac tgaggaagac ctgaagcagc accccgaact
     9361 ggtgcccggt tacatcggac cgatggtgct tggcccgcag gccgaggctg ctggcgtgaa
     9421 gaacccggtt cgctacctga tcgacgcgca cgtggtcaag ggttccgctt ggttcactgg
     9481 tgccgatgag aacgaggtcg actactacaa cctggtgtac ggccgtgact tcaaggtcga
     9541 cggcgtggtg gaagccgttg aggttcgcca cggcgacatg agcccggatg gttccggtcc
     9601 gttgagcttc gagcgtggtg tggaaatcgg tcaggtgttc cagctgggtc tcaagtactc
     9661 caaggcactg gacctgaagg tgctcgacca gaacggcaag gccgtgccgg tgtggatggg
     9721 ctgctatggc atcggtgttt cccgcgtgct ggcctgcatc gccgaaacac accacgacga
     9781 agccggcttg gcttggccgt ccgtgattgc cccggccgcc gtgcacgtgg tggccaccgg
     9841 caaggacgcc gtggcgtttg aaggtgccga gaagctggtc gccgagcttg aggccaaggg
     9901 ccttgaggtt atttacgatg accgcaagaa ggtgtctccg ggcgtcaagt tcaaggatgc
     9961 cgagctgatc ggcgtgccgc tggtcgccgt ggtcggccgc gattacgtca acgacggcac
    10021 catcgaactg cgcgaccgca acggcgaaaa caaggtcgcc gtgcccgccg ccgaagccgc
    10081 tgacgcgctc gccgagcgct tcgccgccct cagttgagtc ccacttactc agcctcggcg
    10141 ttaatcgcat caagcgccat tctgcccagt cgcagaatgg cgctttgctg tggataaccg
    10201 aaacttatcc acattcttaa aaattcaact gcgtcacagt atccacattt gccagttgcc
    10261 cttgtgcact tcaaacgtca tgcctcacag tggaggtacc gctggggcgt gcaagccggc
    10321 aatgaagccg gagacaggct ccggcggtcc acatccaaac aacaggccag caggccgagg
    10381 aaaggaccga tcatggcaaa tcaacaagca gcagtgaccg tcaccggctt tgtggccggt
    10441 gatccgacac taggcggaac cgagagcttc cgcgtgctga cattccgtat gggcagcacg
    10501 cgcagtcgac tcaacccgac taccagacaa tgggaggatt acggaaccgc atgggtgggc
    10561 gtcaaagcgt tccgtgcttt ggccgtgaac gccgccagtt cggttcgccg cggtgaccgc
    10621 atcattgtgg tcggctcact catcaccgag cagtggacca atgagcaagg cgaaccgcgc
    10681 agtagaaccg ttctcgaagc caccaacatt gggcatgacc tgaccttcgg caccacgtcg
    10741 ctgatcaagc cacagcgtgc cggtaatggg aacggaggca atggtggcaa cggtaacggc
    10801 aatgccaacg gtgccgtcga aggtatggat ggcggaaacg ggcaacgagc atcgtcgccg
    10861 tacgttaggc caacgcaata tggcgcgggc ggtgccgcat ccggtgtggg tattgacccg
    10921 tatgcgacct cgtcggaatc tggccccgtc gaatttgggg tcacctccga ttctggccaa
    10981 tcgccgtcgc catcaaccga gtccgcggcc gtggccgatg ccgtcatgtc gcaaccggac
    11041 gccgacacca atcaaccaca gcccgtggcc caacaaacct acgtcgacgg tttcgccgaa
    11101 tcttccaatc ccagtgattc cgaattctga gtgccaacca tacatcatga aaaaccgctg
    11161 tgttcgcctg taatcgaggc aaacacagcg gttttccttt acataacggg gattaccaaa
    11221 tacgcacacg gtccttgggc gcgagccaca tggcgtcgcc ttcggtcacg ccaaaggcat
    11281 cgtagaagag gtccacgttg ctggcgatgc cgttggtgcg gaattcggcc ggagaatgcg
    11341 ggtcgatctg caggtactgt tcggccagct cgtcgcggtt cttggtgcgc cagatggagg
    11401 cgtagctcag gaagaagcgc tgcaggccgg tgaatccgtc catttcgggg gcggtatcga
    11461 gcagtgcctt gatggcggcg ggggagccgt cttcttcggc gtcggactta cctgccgcct
    11521 tgccgagcgc gaatgcatac gccttgagcg cgatgttcac gccgcccaaa tcgccgatat
    11581 tctcgccgat ggtcagcgcg ccgttcacgt gcggtgcctt atcaggctcg tcagcgtact
    11641 tctcggccag ctgcagcggc acaaagctgt tgtactgggc gatcaatgcg ccggtacggg
    11701 cctcgaagtt cttgcgatcc tcgtcggtcc accagttgtt gagcttgccg tcgccgtcgt
    11761 actgcgaacc ctgatcgtcg aagccatggc cgatctcgtg gccgatcact gcgccgatgc
    11821 cgccataatt cgccgcatcc tcggccttcg ggtcgaagaa cgggggctgc agaatcgcgg
    11881 ccgggaacac gatgacgttc atgctcggct cgtagtaggc gttcaccgtc tgcggattca
    11941 tcagccattc gtccttgtcg accgacttgc cgaccttggc cagctgatag ccggtctcgt
    12001 acaggttcgc ggccttggcg ttctcggcgg gcagcgcatc cgcggaaaca ctcagcgccg
    12061 aataatcgcg ccagtggttg gtgtagccga tcttcggcgt gaacttggag atcttctcca
    12121 gagccttggc cttggtgtcc tcgcccagcc aatcggaatt ggtgatggac acgcggtagg
    12181 cgtcaatcag attggcgacc agctcttcca tgcggcgctt cgagctttcg gggaagtgca
    12241 ggcgcacgta ttcgcggccc acatcttcac cacaaatgcc gttgaccagg ctcaccgcgc
    12301 gcttccaacg atcgcgctgc ttcttggcac cggagagcac cttgccgtag aagtcaaagt
    12361 tggtggtgtc gaagtcgcga gacagggagg aggcgaagcc cagaatcatg tgaacacggg
    12421 cccacagctt aaggtcggcc agatcggcct cggcccagaa cgcatcaaga ccagtgagga
    12481 agctcggctc gtgcacaatg acgcgcgcaa acacagactt gaaattgatc ggctgagcct
    12541 tggcggcctc ggtggcgtca taggcggtct gccaggcgtc gatccaagaa ctgagatcga
    12601 agttcttcag cgtggcggcc aaatcggcgt aatcagtggg gttgtaggtc ttcaccgagt
    12661 cgcgcgtggc cacattgtcc caatggttgg cggcgatctt cgtttccaca ccaaggaagt
    12721 ggcgggccat atcaagcgcg gcttcgctgg gaaccgtggc gggagcgttg ctctcgtcat
    12781 cgccggtgtt ggacggcagc tcatcgccac cgttggactc ggcggcgggc gcgtatccgg
    12841 cgttcaccag ctgggtggcg accatcgcca cgtatgcttc gcgcacggga gcatagtggt
    12901 cttcgcgata gtaggcctca tccggcaagc acaggccggc ctgctcaagg tgggcgatat
    12961 tgcggtccgg gtcgccggga tcgccgtaaa cggccaagcc gaacaggtcg gggccgccgg
    13021 ctgggttgat cgtgccgagc acgcgcgtga gagcggcctt gtcgatggcg gaatcgatga
    13081 ggtcaagctc gtcgcggatt gggtcaagac cggcggcctc tatggcgtcg gtgttgagga
    13141 atgagcggta cagggcctca gacttgacag ccgggcagtc gtcggcgtcg aggatgtcgc
    13201 gtacttggtt ttcggcgtct tcggcaagct tgtcgaagga gccgtagcgc gaacgatcgt
    13261 cgggtaggcg gtaggtgtcg atccatgggc cattcacgta gcggaacagg tcattggtcg
    13321 gcttgatgac ggaggagaac gatgccgggt cgatgcccga cgtcagcgtt tcggtttcgt
    13381 tggtcatggc cctaagccta atcacggcgg gggagaagcg ctgatggtgc agatattgcg
    13441 ttatgggcat tgcgctgtca gtgaactaga cagattgcct aattgtggtg atggttcaaa
    13501 ttagacaact tgtctatcat agtatgtgga atctgaacat ggaccttgag ctaaggagtt
    13561 acgatgagca atccgaacga ggaccagtgg aatggccaga cgggccagcc caaccagagc
    13621 cagccgagcc agccgaatcc ggcgggccaa ccgggccaac cgggccaacc gcagtatggc
    13681 gcgtatgaca gcaatcatgg cggccaatat cctccgcaag gtgtgccggg agcgcaagac
    13741 actcagaatc agcatgcgca gaacggccag ccacaaggcg aaccgaacca gaacccgtac
    13801 tacggcccgt acgcctacaa tccgaactat ggcccgaacg gccagccggc atgggggccg
    13861 gacggtcggc ggtatcaggc ggacggccaa tcgaatagcc aagcggacgg gcagccgaat
    13921 ggtccatggg gcgatcaaag tcaacccggc tggcagcaga ccaactggta tgtcggcaat
    13981 ttcagtccat tccgcctgat cgaggaatgg ctgccggaaa aagccaagac caagattcgc
    14041 atcatttacg gcgtggtggg cgtggccgcc atcgccttgg gcgccgccct gttgctggtg
    14101 ccgaacaaga cgctggcttt ggccgcattg ttgctcggcg tgtatttcgt gatttccggc
    14161 gtggtgcgca tcgtcagcgc tctcgtggag ccgggtctgc cgggcggctg gcgtgtgctg
    14221 gacgtgttcg tcggcattct gctgaccttt ggcggcgtgg tggttgtgcg caactacggc
    14281 atgaccggtc agacgctggc attactcatt actttgatgg tcggtttcgg atggattatg
    14341 gaaggcgtgc tgtcgctggt tgaatcgtgg cgtatcccgc attccggctg ggccatcgcc
    14401 tatgccatca tctcgattat cgccggcttc gtggtgctga tgagcccgct gagctccgcc
    14461 gtattcatga ttatcttcgg cggatgcgcc atggtggtga tggggattac ttcagtgatt
    14521 cgcgcgttca ctttcggcaa gcgtcgcaag taatctcacg cttcatgcaa ccagtcccca
    14581 gacaatcaat ccaaagcgcc cgtgcaggct atgtgctgcc gggcgctttt tctcggcctt
    14641 ggccattaat gggctgcgag tggatttcag gtaggcttct ggcggttgac ctcgggctgg
    14701 tctagagttg agaacaagtc tcaacaaggt agaaagaatc tcatgatcga actgaaaacc
    14761 cctcgtgaaa tcgaggaaat gaagccggct ggtcgttttg tcggcggcat tctgaaggaa
    14821 ctgcaggaga ccaccaaagt tggtaccaac ctgctcgaaa tcgacgagtt cgtccacaag
    14881 aagatcgtgg accgcaaggg cgccgaatcc tgctatgttg actacgctcc tgacttcggc
    14941 accggcccgt tcgcccacta catctgcacc tccgtcaacg acgccgtgct gcacggcgtg
    15001 ccgtacgact acagcctgaa ggacggcgac ctcgtctccc tcgatctcgc catctccgtg
    15061 gatggctggg tggccgattc cgccgtcagc ttcgtggtcg gcaaagaccc ggaccccgaa
    15121 gacctgcgca tcatcaagtg cactgaggaa gcgctcgccg ccgccatcga cgtggccaag
    15181 cccggcaacc gtctcggcga catctccaac accatcggcg acgtcgcgcg cgaatacggc
    15241 tacccgatca acctcgagtt cggcggtcac ggcgtgggcc acatcatgca cggcgacccg
    15301 cacgtgccga acgacggccg cgcccaccac ggctacaagc tgcgcgaagg cctggtcatc
    15361 gccatcgaac cgtggttcct caagaccacc gacgagatct tccaggatcc gaaggacggc
    15421 tggaccctgc gtgcctccga cggctcgcgc ggcgcccact ccgagcacac catcgccatc
    15481 accgagaacg gcccgatcgt tttcaccgat cgcaccaacc tctgatcggc gtttggcgta
    15541 tggtaggtgc ctgattggcc tgtgatgggc gatcagcgtc tcattagccg tccgtctgtt
    15601 cgcggttttc ccgcgaattc gagacgtaac aagccgaagt gcgtttgctt tgatagcatg
    15661 agcgcacttc ggctttcgtg tatcgaccgg cattcgccag cggtaacaca gaagacatag
    15721 agtaccgaac actagtgttc gcgaacgcgt gaacgttaat gtttggggta agtggttcat
    15781 gaagaggaag gtgcgcgaat ggcaacggca caacttgatg tcgattcgaa caagttcaac
    15841 ctgcccgtgg ttaaggctag tgcagggccg gatggcatcg tcgtttccaa actgcgcaac
    15901 gatggctggg taacgcttga tcccggattc ctcaccaccg cgcagtgcga atcgaaaatc
    15961 acctacatcg acggcaagca ttcgattttg cgctactgcg gttaccccat cgagcaactg
    16021 tgcgagaact ccgattttct ggaggtggcg tggctgctgc gccacggcga gctgccgagc
    16081 agggctgagt atgagcagtt ctgtgcggac atcaatcaca agacgatggt tggcgaagac
    16141 ttccgtacct tcatgggttc gttcccgcgc tccgctcatc cgatgagcgt gcttgcctcc
    16201 gccatcaatg cgctggccac gttctacccg gacaccaccg atatttctga caacgaccag
    16261 cttgatgagg ccgccaagat catcatggcc aaggcccgta cgattgtgtc gtacatcttc
    16321 cgccgtcgtc gcgatgagcc gatgctgtac cccgattacg cgcgcggcta tgtggacgac
    16381 ttcctgcgca tgtgcttcgc tgtgccgtac gagccgtttg aatccgaccc gctgtatgtg
    16441 catgctttgg gtcgtttgtt gattattcat gccgaccatg agcagaactg ctcgacctcg
    16501 gtggtgcgta tcgccggttc cgcccacgcc aacctgtatt cggcggtggc cgctggcgtc
    16561 aatgcgctgt ccggtccgtt gcatggtggt gcgaatgagg ccgtactgcg ccaattgaag
    16621 gcgattcgtg attccggcaa gtctgtggcc gagttcgtgc gtgatgccaa ggagaatggc
    16681 cagaagattt ccggccttgg ccaccgtgta tacaagtcgt atgatccgcg cgcggcgatt
    16741 gccaggaagt atctggaaaa gatcatggag cgcgaagata cgcagaagct gccgtctgac
    16801 gagcgcgcgc tgttcgccgt ggccaccgag cttgaagata ttgcgctgaa tgacgaatat
    16861 ttcgtatcgc gcaacctgta tccgaacgtc gacttctaca cgggcttgat ttatcgggcg
    16921 atcggcttcg atccggccat gttcaccacg ctgttcgcgc tgggccgcat tcccggttgg
    16981 attgcgcagt accgtgagat gttggccgac ccgaatacca agatcggccg cccacgccag
    17041 gtctacaccg gtccggtcga gcgcgatttc gtgccgatgg acgagcggtg attgttggtt
    17101 tgagaaagcg cttcgctgga gattccagtg aggcgctttc tatacgtaca gcgcaaacca
    17161 gctatgctgc gacggctgca taagaactag cagggctagt tctgaaattg aaataatgtg
    17221 tgcggtatcg aggcgcaccg agggaaggcg tacgaaggta cgtcgaccga ggggcaacga
    17281 agataacgct ccattatttc aatttcagtt cttgtgcagc ttggcgttga gctcaattcc
    17341 caccttgcgg tagcgggctt cgatgcggcc gttcaccgag ttgcggataa acagaatgtt
    17401 gtctttgccg gagagctcag cacccttgac cacttccagc ggttcgccag cggcggcctt
    17461 ggccttgtcc cagatggtga tcttagtgcc ggcggtgatg tacagaccgg cttcgacggt
    17521 gcagttatcg cccagagaga tgccgatacc ggcgttggcg ccgagcagcg agtgctcgcc
    17581 gatggaattg cgcagcttgc cgccaccgga caaggtgccc atgatggagg caccgccgcc
    17641 gatgtcggaa ccgtcaccca cgaccacgcc ttgcgaaaca cggccttcga ccatggagac
    17701 gccgagcgtg ccggcattga agttcacgaa tccggcgtgc atcacggtgg tgccggaaga
    17761 caagtaagcg ccgaggcgca cgccggtggg cactacgtaa tcgatcatgc gcgggaactt
    17821 atccacaccg aacacgttga cagtggcggc aggagctgcg gcaggcagtc cggcctggga
    17881 ggcgaaagcg agagcggctt cggtggaggc gcgttcgacg tcggccttgc gcagcgtgaa
    17941 gtcttcaacg gcgaaggggc cgtagttggt ccacaccaca ttggcgagct gggcgaagat
    18001 gccgtcgagg ttcagcgtgt tcggcttggc catgcgcatg ctcatcaaat ggagcttcag
    18061 gtaagcgtcc gcggcattac tgatcggctc gtccaactgg ctgacgacga ataccggcac
    18121 gcggcgaatg ccacgggcgt cggcctcatc gtgagccaaa gtaccgaaat tgtggttcgg
    18181 gcgatcggac tcggccggtg cctcaccgag attcagttcg gggtaccaga cgtccagggt
    18241 ggtgccggcg gcgtctacgc tggccagacc ccagccccat gcggtacgct cttcgctcat
    18301 agttgctcct tattgttgta cgcgttagtg cacgcttgtg gacgcgcgat gcgattaatg
    18361 attcccacct tagccttatg tgcgcctagg tgagggacga tgcccgcgaa atggcttgaa
    18421 atcgtatttc ccggtaaaaa attaccgagt gtgattggcg atgggatgat aggttctccg
    18481 gtgaaattcg ctggtgaaca actcgacaag gaggtgtgcg atgtgatgtt gcatcattcg
    18541 ggagcatagg ctgtggccat gcgacgcccg gcatcataga gtttgcgctt gagcgaatcg
    18601 ggctcaagaa tcactacgcg gccatcccag gaatactgca atgcccacca gaacacagcg
    18661 cgttcgggag catcgacgat gacttcgaac tgtgtgccgt ccggtgaatg tttgatttga
    18721 gggtcgtcga accattcgaa cacgttgttg agcatggatt cgtctcgtac ggccatgcgg
    18781 atgcgaatcg gggcatcggc tacggggtat ggacggtggc gcatgaagtt cactgcgtca
    18841 aaatcagctg gtgctggttt ttccagtggc actgtgccgt tcctgccttt ggcgctgacg
    18901 ttggcgattc ggttgaccac gaaaatgcgg aggttgtctt cgccatgcag gtggcagatg
    18961 aggtaatagc gtccgttctt gtacaccatc tggtaagggt cggcggtgta ggtaattcgg
    19021 cctgcacccg tacggctttt atgcggtatc aaggtgccat ccggtccgta atcacggtat
    19081 tggaaggtta cgccgtggcc ttcttcgatg gcttgattca gctggtcgat agtggatagg
    19141 aactcaccgt tgatatgcgt gtaggtggcc acatggtcca ggtagcctac tgcctgcccg
    19201 gcggcgcctg cgagctcgcg gattttaccg atgaggtcgt ccatggtgtc gaggctgatg
    19261 cgagataggg taagactgtc tagtagcagt cgcatttcgg cagtggacag gaatgcatca
    19321 agataccagc cgggttgtgg attcttgcat tcgatgccgg caaggtcgct ttcggtgaga
    19381 tgcccgatat gtctgccgag aaattccgtg gaggtcagct ttttgagctg gtttcgtatg
    19441 gtcttctcgg tcggcagctc gcttggatct tccagctggt gtttacgggc aagctcatcg
    19501 agtatttgcc gcacggtcag gccatgccca aagtaactgt gacgccacag cacctcgaag
    19561 atttcgaggg tggtgttccc tgcataggat ttgcgttgcg ccggcttgtc tgcatccata
    19621 cattaaggct accgcgcgct tcgtctagga tggaaacgtt gggttgaaat acattcgctg
    19681 gaaaggaggc tcgcccatga gcaaagggta tggtgtgttt ggccatgttc ccgataatgc
    19741 ggatgacgat ggcctcctgc cttatgagga tgatgattcc ggcgtttcct ggggccatgc
    19801 gttgcgtcgt cgcaatcctt ggaaaagagg agacgacgaa gatgccgatt acgacgacgg
    19861 tcggtatgac atagatgaca ctgatgacac taatgggggc aggggcatct atgccggtaa
    19921 aagcggcgga gtaggcggca tttctggtga gacgggtgga atacttggta gctcaggcgc
    19981 ggccggcagc agagacgtaa cgtttggtgg aacggacagt ttgtccgccg ccgatgcggt
    20041 gatgccctat cgtttcaggc aaggacgttc cgtatggttc gacgatgagg acgaaggcca
    20101 tttcgccggg tctgcgaagc gtgccgaagc ccatgccgtc cctatccttg cgttacgcaa
    20161 cgatgtggaa tcgtcatcgt cgaatgccgt gctcgctcgt gcccgtagca ttgcgcgcaa
    20221 agcggccacc atggttatca atccggtata cagcgaaaat catcgaaccg ggtatgccga
    20281 gcttgacggt attctacgtg gcaccaccag cccatcgagt aggtatcgta ccagcgtgca
    20341 attcgacctc gatacgggag aagccgtcgg tggcaagtgc tcatgcccgg cttacggccg
    20401 tggctatggc atgtgcaagc acatgattgc gctcgccttc atattctgcg atgatccgca
    20461 gcgttttgaa ggctaccgtg ccggggccgc acgaccgtct cggacgctga ttgactatat
    20521 ggagcacgcc gataggcaag acgctcaagc caaaattcgt cgtcgcaatg cggttatgcg
    20581 tcggtttgat gccacgcaag ggaaaaacgg tgcaaatgct cggcgtacaa aaagcggata
    20641 cggctctcgt gccggcaccc gaggcggcgg gtatcagggt gacagcgaga ctgtgggctt
    20701 ggggcaggtg cagcttgtcc cgatattgag tttcatcgac ggcgtgtgga gcgtggagtt
    20761 caagatcggc tcggcttcca atgggtccag ttatgtgctg aaatcgattc cgcaatttgt
    20821 gcttgccatg gcgaacggcg actatgcgga ttatgggcag aagcttgcct ttattcatac
    20881 gccggatatg ctcgatgatg attcgcgccc cctgctggac gtccttgatg atgcgatggc
    20941 cgtgcgtcgt gcggccgagc agcaggacag gtattacggg catatcgcca tcgaccgtca
    21001 tctgacgctt tcagcaccgg aagtggcccg gttgctgtcc gtacgtgagg gcagaggcgt
    21061 acagctcgtg ctgaacacat ggttctccgg gcagcctgca tcggtgccag ttgatggtgg
    21121 cgagccagat tttctcatgc gcattgcgcg gcgtgatttc aatacgtatg gtccggatgc
    21181 cggatacctg cttaccggag tgccagcggt ggaaaccgtg attgacggcg gcaagaatgt
    21241 atggctgttg ctcgcctcca ctaatccggc cggtgcggta tccgccatgg tggcgggctc
    21301 tcagcgtaaa agcgcagcag gatgccgatt cgtccgttgc ccgaaacgat tgattccgtt
    21361 gcgaggacta ttgggagacc tgtttgaacc cgatggcgag ggacaggtca ttgccggcga
    21421 tgacatcgcc ctattcgcac gcacgctatt gcctcagctg gttggtgccg gactgttgga
    21481 ggccagtgag attcctcgcg agctggctga attaagtcct acggaatgca gcctccagtt
    21541 ttatctggat cgtgatgagc atggtgtgca atgcgaggtc aaggcacgct acggcgatgc
    21601 cattgtcccg ttgctacccg atggcccgac ggtgcatgct gagggcgagc gttccatccc
    21661 cttgtctcgg ggcgtgattg ggcgtgattt cgacgctgag catcttgcga ttgatgtagt
    21721 gcgtgaattc tttaccatgc ctgaccagcg caagcgcgtt gcggccgcca ctcatcagac
    21781 ggtgaatggg ttccgaacgc cggccgcgcg aaaggccacc aaaaaattcg tgtctgatgc
    21841 ggccactatc gatcgcgatg acaccacgca gatcgttcgt ctctttgacg agggattgcc
    21901 ggcactaaag gaagtagggg aggtcttcac cacaccggct tttgaccggc tgatcgcgcc
    21961 caagccacca agcgtcaaag tcgggttgtc catcaaaggc aacttggtgg agatatcacc
    22021 cctcgctgat gaagtgccgc cggacgaagt gggtgccttg ctatcttcgt accgtcggcg
    22081 tcaacgattc catcagttga aggacggcac gcttgtcaaa ctcagcggcg cgaatctcag
    22141 cgcgttggac cgattggcca gcgacctcga tctgagcgag cagcagctca actccgggct
    22201 gatcgagttg ccgggtggtc gagcattcct actggatggt gagctgccgg atgacggctc
    22261 cgatgtagtc aaggacgctt cgttcaccga atacatcgac gatctcaaga tcatcgatcc
    22321 taagtcgtat gaagtgccgg atagtctcaa gcatattctg cgcccttatc aggtcgaagg
    22381 cttccaatgg ctgaacacgt tgtgcgacaa gggttttggc ggcattctgg ccgatgagat
    22441 gggcttgggt aaatccgtgc agcttatcgc gttgctcctg tctcgatatc agcggaatac
    22501 cggcgagatg ggcgacggca gccttggccc atcgctgatc gtgtgcccgg cgtccttggt
    22561 gtacaactgg ggtgccgaat tcaccaagtt cgccccgagc ttcaatgccg tggtggtggc
    22621 cggtaccaaa gccgagcgcc gtaccgccat cggccgtgcg ttccgggcgg atgaacccac
    22681 cgtgctcatc acctcatacg acttgctgcg ccgagatgtc gatgattaca ccgccaatga
    22741 gcaacgtttc aatgtcatgg cattggatga agcccagtac atcaagaatc acaccacgaa
    22801 aatagccaaa gcggtcaagg ccgtggtcgc cgaccatcgt ttcgccttga ccggcacgcc
    22861 gatcgaaaac nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntcaag
    22981 cgcaggctga agtcccaggt gttgaccgat ttgccggaca agctagagac cacgctgacc
    23041 gtgcggcttg ccgacgaaca gcgcaaactg tatgcggctc acgagcagcg gctcagaatg
    23101 cagctcgaac acagcgaaga agccgatttc aacacgtcga agattcgcat cctcgccgag
    23161 ttgaccaagt tgcgccagat ctgctgcgat ccgaggctgc tgtacgccga tgccaaggat
    23221 caatcggcca agcttgccgc catcaccgaa ctggtggaaa cctgtgtgaa cgaaggcaag
    23281 aaggcgctga tcttctccca gttcaccagc ttccttgatc tgatcgccga acgtttcgac
    23341 gcgcaaggcc tgcgctacta caccatcacc ggctccacgc ccaagaagaa gcgtctcgaa
    23401 ctcgtcgacc agttcaatgc ggacgatacg cctgccttcc tgatttccct gaaggccggc
    23461 aacaccggac tgaacctgac cggtgccagc gtggtcatcc acgccgaccc atggtggaac
    23521 gctgccgcac aggatcaggc caccgaccga gcacatcgta tcggtcagac cgaagacgtc
    23581 aacgtgtatc aggtggtggc caaagacacc atcgaagaac gtatcttgga gcttcagcat
    23641 actaagagcg agctcgcccg ccagttcacc gacgcttcct tgcttgccga cgaagccggc
    23701 accggcgcca gcgccctcac cgaagccccc gcctccatcg ccaccctcac caaggacgat
    23761 ctcctcaatc tgctgggatg agaagattct gctcggtgct cttgagcttg gggatggaaa
    23821 cagtctgggc tttgtggacc cgttcgctaa gccgagaccg gagtcgatag cggtgtggca
    23881 tggttcgtgc gtggggagcg tgattcagtt gttggctgag gcgattcgga cgctgtcgtt
    23941 ttgtgctttg ttttgccgtt gaccaattga aatgctcgtt ggtaggagac ccccaataaa
    24001 gctccgattt gtcgatacgt gagtccctcg ttgcgtaggg cctgtgcggc gagtcgggat
    24061 tcggcagccg cttgtgcgtt ggcttttttt gctatttcgc gctgccggaa catttctgtt
    24121 acgtgttgtt tggcttgttt cggcaacaca aacgtcacct tgagctccgg attgtcgtcg
    24181 tcgttcatga cagtgatgag atctttagcc attggctcga tatcgtcaag tcctttggcc
    24241 tgggtcgacc gttcgatagc tggtacctga acgtaccaat atcggtcatc gggttgcaca
    24301 agcacgtcat agttgctcat tgccaccacc cctttccgag tttctgctcg ctctcgttga
    24361 tgatatccag cgtggttcgt tctgagactt cattatggcg ggggatgggt atcctcaacc
    24421 cgtccaggca gtaaacctca tgtctgctgc cttgccgtaa caaatagaaa tcgagccctc
    24481 gctgctttgc cgcttgccga agtttcttga tgatgagttt tcttcgtgtc atcataactc
    24541 tcaatcccgc actagacaaa atgcgtatgt cgaattgccg ggtgggagtt gtgggtaaac
    24601 gtagagatgt tcattggtcc ttctttctgt tgtctgacgc gtcgttgcgt gcgggtgcat
    24661 ttgacggtat gggatgaggt ctctgattgg caagctgaac gggggtatgt ggttctagct
    24721 tccgagttat ccacattgat aaggtgcttt tttaaagtga ctttcccgtg ttggtcgtgg
    24781 atgctgcgag caaggcaaga acggttgatc acgtcgcgat gcgtgtgggt gaatggtgaa
    24841 agtgtttttc gatatcttta tatcgatatt cgatatatta tgatggtggc atacggtgct
    24901 gtgcaggttc ctccattgat attgatattg gattaaggag catagccatg aacgctggta
    24961 tcgcattcat ttctcgtaaa tctcgcaaaa cagacgtcgc ttggcgctat gccattgccg
    25021 tggtgcttgt cgtgtgttgt attgggctcg catatgtgga gattgttgtg gcgccgtggt
    25081 ttatcaatga ggtggtgtct atgtttccgg agaccacgtt gcttaaagaa ccgtccatgt
    25141 tctgggaatg ggcgattctg ctcgccgttc aggcgacttt gctgtgcacg gcggcgctac
    25201 agcacctgct ccatgccgat gtgatatgga gtgagtccca tgcgttcgta gaaggcttgg
    25261 gctttggggt tgcaggccca gacgttgagc gttacgttgt ggcagccgga ttgacgggcg
    25321 aagtcgagca cgtagcgata caaggtggag cctacatggt ggccgcggga ccgctcgtca
    25381 acgcatagat catcgatgta cagggttttg atatcggtca ggatattgct gttgaggtgc
    25441 tgctggaaca cgcagaatgc gtagccaagc acttggtcgg tggcggggga gtcggcgtta
    25501 tgttcggcgt cagtgccgtc attggcagat ggcgcaaccg ccacgaacac aggggtctga
    25561 tcgttggtca gaatcgcctt caattcctcg tccgtgtatt tcttgacacc gcctttgaac
    25621 agatccggtc ggccggcatg atgcacttcg agcacctgcc gcagcagttc gtcgatttgg
    25681 ggaatatcgg ccgccgtggc cctacgaatc gtcatgtcat ccattgaact cagtctaatc
    25741 gggcagtctc gaccggcaga tgatcattgc ccgcgataag agacacggac gggcaagatg
    25801 ggtgatcgtt cgttcagggt ctcattctcg ccgcaacgtc acggtagtct ggagtgcgtg
    25861 aatcgcaaga aacagccagt ggagaagtat gagcggggta cccgttcgta cacgatgtcg
    25921 catattcgtg gcaaagacac caaaattgag gtactggtac gcagctacct gtttcgcaag
    25981 gggttgcgat tccgcaagaa cgacaagcgt taccccggac acccggatgt agtactgccg
    26041 aaataccacg ccatcgtgtt tgtcaacggc tgtttctggc acatgcatga aggatgttcc
    26101 aaacattcga tgccgaaatc gaacgtggaa ttctggcagg cgaaactact gcgcaatcac
    26161 aatcgagaca tcgcgcagcg agccgaactg gaggccgctg gctggcgtgt aattaccgta
    26221 tgggagtgcg aattaacgaa agccgtgcgc gacgaacgcc tcgcacggct gtatgagcag
    26281 attgttgaag gagatgcaga ttcagcggaa taatttcagc tcccctctgg aggggagctg
    26341 aaatcaggat tcaaacggga acttcaggcc ccaacggtca cgaatcgcat ccatcagctc
    26401 catgatgcgg atggtgtcgg cgtgcggcat cgcctcgcat tcgatcttgc cgtccagcag
    26461 cgcgttggcc gcatcggcca cctcatactc ataaccggtc aactgcggcg gcaccgcaat
    26521 gctgcgcacg ggcttgtggg cgttgtcata gatgtcgatg gactcgggat tgttgatatt
    26581 ggtgaccacc agataaccct cggtgcccca cacatagccg ccgcggtcgg aggaaaccag
    26641 catcgaactg gtggacacgg ccatcgtgcc gtcgctgtaa tacaacgtgg tggaattctg
    26701 cgcatccaca ccggtttcgg cgggcaccat cgacgtatcg aaatgatcaa gtgtacggcc
    26761 atggtcggcg ccgaccgcca tatccaggaa gttcagcgga tacacgccca catcgagcag
    26821 cgcgccaccg gcacaggccg gatcggtcat acgtgccttg ccggacaccg gatagcacag
    26881 gttggcgctc gccgctttca cctcgccgat ctcgccggac tcgataactt cgttgatgag
    26941 cttgcggctg ggcatgtagc gcgtccagat ggcctccgtg cacagcatgc cggtttcctc
    27001 ggccacggcc agcgcctcac gtgcctgccc ggcgttcgcg gtgaacgact tctccaccag
    27061 cacgttcttg ccggctttca tacacaggat cgcctgttcg gcgtgcagac tgtgcggggt
    27121 ggcgatatac accagatcca catccgggtc cgccaccaac tcctcatacg atccataagc
    27181 cttgtcaaaa tgccattggt cggcaaaagc ctgcgcgcgc gtaatatcac gggccgctac
    27241 cgcatacggg tgaatccatg aggaatacag tgaatcttca gccatctggg tcagggtatc
    27301 ggccatggtg tgagctatgc ggcccgcacc cagaatcgcg acgttgattt tttcgccgct
    27361 ggcctcgaac tcggcgcgct tgccattgaa tcgactcatt attcctcctt gaatgcgggt
    27421 tggtgcactc aatcgaccta cagtgattat agaagaagaa accttgtccc gtgaggcgga
    27481 tagaaccgaa atatggaaaa cgaagcagcg cagcagcccc aatctccggc cgcgtctcca
    27541 gtcatgtccc gccgagcgcg tgaggccaat cccttccgtg ccatggtatt cggcgaacaa
    27601 gcagacaaga tgatcgccca aggtaccagc gtcatcaaat taagccttgg cgagcccgat
    27661 ttcggcgcgc cgcccgccgt acgcgacgcc atgcgcgaac agtacgacgg ccgggccctg
    27721 ccgtacaccg ccgccatggg cctacctgaa ctgcgtcagg ccatcgccga cttctaccgc
    27781 gagcgtcatg accttgacat cgaccccaag cgtatctgcg tgaccgccgg cggttcggcc
    27841 gccctgctgt tggccaccgc gctgaccgtg gaccccggtg acgatgtgat tgtggccgac
    27901 ccctcctacc catgcaaccg cgaacttgtg cggtcgtttg aaggccgggt catcgatgtg
    27961 ccgacctcgg ccgccacccg cttccacctg accgccgacc tgtgcagtca gtactggacg
    28021 agcaagacca aggccgtaat gattacgtcg ccgtccaacc ccaccggcac caccatcgac
    28081 ttcaacgtat tggacgaggt gtgcaagttg gccgccagcc gcggcgcatg gcgcatcgtg
    28141 gacgaaacct acctcgactt ggctgaccgg gagccggacg gtaccgaagt caaatccgtg
    28201 ctggcctgcg accccgatgc catcgtatgc aacagcttct ccaagttctt cggcatgacc
    28261 ggctggcgcc tcggctgggc gattctgccg gacgagccca ctgtgctgga agcggttgac
    28321 gatctggcca ccaactacta cctgtgcgcc cacacgccca cgcaacatgc ggcgctcgcc
    28381 tgcttcacgc cggaaagctt ggcggaatgc gaagctcgtc gtcaggaatt gctggcccgt
    28441 cgccgcattg tgattgacgg gctcaagcgc atcggtttgc cggttgaggt ggagccgaat
    28501 ggcgcgttct acgcctactt cgacatctcc cgcactggtc tggatgcctg gacgttctgc
    28561 gaacgcgcgc tggatgaagc ccatgtggct ctgactcctg gccgcgactt cggcgcggcc
    28621 acggnnnnnn nnnnacggcg gatacgcatg tgcgcctgag ctatgccgcc tcacgtgagg
    28681 ccctgaccga ggggctggaa cgtctcggaa cgttcgtcga atcgctgtga acggcgcggc
    28741 agccggtgtg attgttgtac agtagtcgag tttgtgtgat gttcggctgg cctcacggtg
    28801 ttggttgccg gccgaacgac tcgccatggt ggcgcacggc tggctgattg cgatggtcgg
    28861 cccgttgttc ggccatcacg ggttaggtaa ggtcaggaat aggaatcatg gtcaatctgc
    28921 gttcgcatac cacgcgactt ggtgtgcttg atatcggttc gaacactatc cacatgctta
    28981 tcgtcgacgc cgcccccggc gctcgccctg accccgaggc cagcaccaaa tccacagtgc
    29041 gcctgatgaa gtatttaaag gacgacggct caatcaagaa gtccggcatc gaggcgattc
    29101 tggaagccgt gtcccagtgc atgcggctgg ccgaggaata cgaaatcacc cagctgctgg
    29161 ttatggccac ctccgccctg cgcgaggcgc cgaacggcaa caaagtgatt cacaagatcg
    29221 aggaactgat tggtcagggc gtcactgtgc tgtctggcac ggatgaggct cgcctgacct
    29281 tcctggccgc ccgccgttgg tatggttggg atgccggccg actgctggtg ctggacatcg
    29341 gcggcggttc gctcgaagtg gcgatgggct ccgacgaaga gccgaccgta gcgctttccg
    29401 ccccggctgg cgcgggccgc gtcaccactg aatttctgcc ttccggcatg gccacgcccg
    29461 acgagttgga agacgtgcgt aagaacgtgc gcaagattct ggagccgatg gtcaaggttt
    29521 tcccgcaatc caagcacccg aatcatgcag ttggcacctc caagaccttc cgctcgctgg
    29581 ctcgcctgtg cggtgcggtg atgcgtgcgc cgggccgcga agattcatgg attatgactc
    29641 gtgagcagtt ggaagactgg attcctcgtt tggcggcgat cgcgcctgag cagcgcgtgg
    29701 ctttgcccgg catcacgcct gaacgcacga tgcagattgt gggcggcggc gtggtggccg
    29761 acgagatcat gaaggccctg aatattcagg aagtcgagat ctgcccgtgg gcgctgcgtg
    29821 aaggcgcgat tttgcgctgg ctcgaccaat tcggccgcac ccgcctcggt ttctagtcct
    29881 attgagggga atcacccaac cgccacggta gccaacaatg ccgcatgatc agagccctcg
    29941 actttgacca ccttgcattg tccggccttc atgccctgat caagcaccac gtggtcaata
    30001 ccggcgaaca ttggcacagc cgcacggttc gtgggccatg agaacgtaaa cccatgtccg
    30061 gattcacgtg cggcatccac gaaccggtcg ccaagaaaat cacggaatgg cgtgtgatcg
    30121 taagtggcgt tgaaatcgcc catgaaaata taccgtgtat ccgtatgctc gcgcatcaga
    30181 cccaactcat ccagcgatcg nnnnnnnnnn ccgggtacgg gagcggtggt gtgcacggat
    30241 acgaatcgaa tctgctggcc acccatatcc accgtgccgc cgggcatgaa tgaggcgctg
    30301 gaattcacgt catcgtcggt cggatcggca agtggcgtgg ccgaccagag gccgttaccg
    30361 aatacgccgt cagacgatga gacctgcgca tacggcagat aatgctcaat accagcctca
    30421 ttgagttttt tgacgaaatc ctcggtggtc tcctgcaaag caagcacttc gacgcgctga
    30481 tcgcgaacca gctcgacgat ggcttgcggg tcggcctgcc ccttatacac attgaaggtc
    30541 atgacgcgcg cgtaggcatc gctggtgttg ggggaggctg cggccacggc gttctgcgcg
    30601 gcttggggga gtggatcggt cgagtagaag aacggatatt gccaatagcc attgcatgcg
    30661 attgcggcga tggcgattag tgcggcaagg atacgccgcg acacgatggc caacagcagg
    30721 gcaatcaagc caagcaacgt gaaccatggc gttgccgata ccacaatcgg cgcaaatggc
    30781 agttcctgta aatcggcagg cagcgcgcgg gcggcagcgc cggcaagggc aagaagcgcg
    30841 aacatcgtgg cgagaaaacc aagcagacga tgccgtttgt gtggcttcac ggcagacttg
    30901 gatactgggc gtgccgtggc tctggcagag cgtggcttgg cagcggcgcg agctcgtacg
    30961 cttgcgcctg tggtagtact ccgtgccttt gccatgctgc cctttcctcc ggcttgttta
    31021 ctgttacttt gcttttcgtg cgatttgcca ctgtaccgtg tacggcttgt ctttcagtgt
    31081 cttttcgccg tgcgcaagag cagttcgcac caaactggca cgaaagaccg ggccatgacc
    31141 tgatgtcggg ttcctccatg tggcttgctc aggttgccgg atggcttgcg caagctgcca
    31201 cgcgaacatg atgcgactgg gcgtgtggcc gtgtacaatg aaacgtcgtt gcccgagtag
    31261 ctcagtggat agagcaccgc tctcctaaag cgggtgtcgt cggttcgaat ccgatctcgg
    31321 gcacttggct tcggaaacat gacggtatct cagcctctat tcatcggtga tgagccatgg
    31381 gcattaggca tgtgtaaacg tataggttcc actatccatc atcccgtcgt gtgaaactat
    31441 gcaaagacat gtggggcttc acggttgtcg tgagggtcca tggctatcga atacacggaa
    31501 gagaagcggt ttactgttga gcaggttgag gcgctgttca agtcggtagg ttgggaatcg
    31561 gcgcagtggc cggaacggtt gttcaaggca ttgcagcatt cggatacggt gatcagcgca
    31621 tgggatggcg gccatttggt cggcttggtt cgcgtgctcg acgataccga aatggtggcc
    31681 tatatgcatt gggtgctggt agatccggca tatcacgggc agcatatcgg cagccatctc
    31741 gttgaactgg tcaaggctaa gtatcgcgac tacttcttgc tggaagtcat gcctgaggaa
    31801 agcaaaaacg caccgttcta ccagaagcat ggcttccacc tgatggacga tggccgcgcc
    31861 atgcaaatcg tcaatcgcgg ctgacgggct ggcagtgcgg acggcgcaag agcggcggca
    31921 tgcgccaatc gtgggcaacg tacgtagtcg ttgacggtta tcccgtagca atgttcacaa
    31981 tacgacccct tgtccataca gtgaaaataa cggctgacgt tcgacgttgc tgcgctatga
    32041 ctagagacca tgagtgaaga caaacagtat gacttctatg tggccggtcc gttctttgat
    32101 cctgaacaaa cggcaagtat ggaacgtttg gaaaagatat tggaagacca tggcaaggta
    32161 ctgttcaagc cgcggtttgt cagccagatc gaagaagtag gccctgaagg atgctttgcg
    32221 gacgatatcc atggcattaa tgctgctaaa gccgtggtcg ccaatcttat ggacgaggac
    32281 ccgggcacga tgttcgaaat cggatatgcg catgcgctgg gaatcccggt gtacggctat
    32341 ttcgaaaacc ttacgccaat ggatcgtgtc aatctgatga ttgcgcaagc ggtcgaactg
    32401 gtgttcgtcg gtcctgatga tgtggcgaaa tacttggaaa ccggcgagca tacggaagtc
    32461 gattacatcc agttctaaac agctctaaca ggttctctgc aagcagggtt ggcggcgttc
    32521 tcgtacatag tcatgtacgg gaacgccgca gtggtatagg gatattccaa caagcggcat
    32581 aatggggtcg tgggaaaacc agtcggatac aagtgaagtg tctgaatttc gtaaggagtg
    32641 ccgttatgag taaccacaat cccatgaata ttgccaccga ctttgccaag gccacgggag
    32701 gaaccgctcg caaagttgtt gctttgaccg ataaaggtcc gttgaatggc tttaagaaat
    32761 tcctgtcgcg cggatcgatg attgatatgg cggtaggtgt ggtgatgggc tctgccgtta
    32821 ccgctgtggt caattccatc gtcgacaatc tcatcagtcc attaatcggc atgattggcg
    32881 gcgtaccgga tctttccgga ctgctgacca tcacgttcaa caactcgact gtttcatttg
    32941 gtgcgattct taacgcgctg attaacttcc tgcttgttgg cgtggcggtg tatttctgcg
    33001 tgattttgcc gattaataag cttcgtgatc tcactgctgc ccaggatgag gctgacgctg
    33061 aagaacaggg gcctagcgtt gaagagcaga ctctcgccac gctgcaggag attcgcgacg
    33121 agctgaaaaa ggccaataac tccgccgagt aatctgctga atgtacggtg cgacagattt
    33181 acctggtttg cccgaaataa cggattgccg gggccatttg gtctggacaa tccgttgttt
    33241 cgcgcgtgtt ttgtagcatc cgtggagtta catggggagt atgactgaaa acatcgctga
    33301 taaggccata aacgatactc gtatcgtcaa gcagttcacc aatcccgtca aggagccgat
    33361 tgacagcgac atcaacctgt tcgatctgct cgataaccgc gccaagcgcg atcccgaagg
    33421 tgccatgatc gaatacaagg gcgatgatgg cacttggcac ccgtacagcg cccaagtatt
    33481 ccgtgacatg gtcatcgact tggccaaggg cctggtcgga cttggtgtca acaagggcga
    33541 ttcagtcgcc atcgtctcgc gcacccgctg ggaatggacc gcgctggata tggcgatcat
    33601 gtccatcggc gcgctcaccg tgccggtgta cgagacccac tccgccagcc aggtcagctg
    33661 gattttcaac gattccaaag tcacgctcgc catcgccgaa gatgacgggc agcgtgacaa
    33721 gatcgaatcc gtgcgcgatg aggtgcccac cctgcgcaac gtgttcgtaa tcgaggccgg
    33781 cggtctgaac gccatcaaga cctacggcga aagcgtcacc gacgccgaat tctgggaata
    33841 caaggaagcc tcccacggcg acgaccgcgc caccatcgtc tacacatccg gctctacagg
    33901 caccccgaag ggcgtggaac tgacccaccg caacttcgcc ttcctggtgc tttccgcgct
    33961 gcagtacatg cctcgtgccg gcgcatggcc caaccgccgt ctgctgctgt tcctgcccct
    34021 gagccatgtg ttcgcccgat tcctggaatt cttcagtttt ggcggcacga tttcgctggc
    34081 cttgagctcc aatatgaaga cgatggtcaa ggacttcgag accttcggcc ccacgctgct
    34141 gcttgccgtg ccgcgtgtgt acgagaaggt ctacaatgcc gcctcccagc gcgccggcac
    34201 cggattcgct ggcaagatgt tcatgcgcgc cgccgagaac gcgcgcgaat ggtccaaggc
    34261 cgagcagaag ggtgagcagc tgccgattac cggacgcatc gcccacgcct tctacgagca
    34321 ggtggtgtac aagaagattc gcacgatttt cggtccgaac gccgacttcg ccatcaccgg
    34381 cggcgctccg atggactccg agctttccca cttcttcaac ggcatcggca tgccggtgct
    34441 ggaaggctac ggcatgaccg aaacctgcgg cccggtgtgt gtgagcttgc cagaggacaa
    34501 ccgcatcggc accatcggca tgccgatgtg cggtatcact gcgggcatcg ccgaggacgg
    34561 cgagctggtg gtcaaaggac cgctggtgtg caagggctac cacaacaacc ctggggtcac
    34621 tgcccagcag atcactgatg gctggctgca caccggcgat ctgggcgata tctccgaaga
    34681 tggcttcatc tccatcaccg gccgtaagaa agacctcatc atcaccgctg gtggcaagaa
    34741 tgtgtcgccg ggtctgcttg aggcttccgt gatgacctcg cctgtggtca accagtgctt
    34801 ggttattggt gataagaagc cgttcgtggc cgctctggtc acgctggatc tggcggatgc
    34861 caacaattgg ctggaatccc agggcgccaa accggaaccc gatctggcct cgctggccaa
    34921 gaacgccatc gttcacgctg aggtggagcg tgcggtgaac gccgccaacg agggcgtttc
    34981 ccgcgccgag tcgattcgta agttcgagat tctgccagac gaattcaccg aggccaacgg
    35041 catgctgacc ccgagcctga agacccgtcg cgcgcaaatc gttgagcact atcgcgagct
    35101 cattgacgac gtgatctacg tgccgttgaa gaagtagcta tcaactccct ctgatccaaa
    35161 aaataagctc tctctgacga gggaactgtc agccgcaggc tgactgggag agaccgcggc
    35221 tagcccgcga aacaacaagg ccgggggcgg cttgcacccg gcctcagctt attcagtcca
    35281 aaatcgccga aacataatcg gcggcggcct gatccagctc cgcatcgccg atggtggcgg
    35341 tgccctgcac caggaacggc tcagcatagt tcatgccgac cttgtacgcc acgacctcaa
    35401 acgggctcag cagctcctcc atggtgtggc tgtactcgcc atccttgcgg tacttggcgg
    35461 cgtcggaacc agtggtgacc accagcttga gctccttgcc cttgagtgcg tggcctccac
    35521 cgtaggccca gcctgcggtg atgacctcgt cttcccattc cttgagcagc gcgggggagg
    35581 agtaccagta gaacgggaac tgaaggatta cgcggtcggc agcctccagc agctcgtgct
    35641 ctgtgtcggc gttgacggag aagttcgggt attcggcgta ttcgtcgcgc acgatgatgt
    35701 cgtcctcgcc ctgtgcggtg agttcggcga gcaggcgggc gttcacgcgc gagccgtcct
    35761 taagatgcgg atggaataca agaatcaacg ttgacatggg atttcccttc tataaactgc
    35821 tgccaatgtg gcctaatcac cactatcgta tgccgggaag cagaactcag tccagtccgg
    35881 actcaggtgc gagccgcggg atgccgcgcc gggaggcaac tcagtcgagg ttggactgcg
    35941 tgcctgcggt ggcttcgttg gcggcttgct cggcttgtgc ttggtcgagc ttggcacgga
    36001 cgtcgttgag cagaccctgg gcgcgcttag ccaaggcctc gccgatctcc cattgacgca
    36061 tcgactggtc aagattgagg ccgccggttt ccagtgcctg gacagcctga atcagcttgt
    36121 cgcgcgcctc ctcatatggc atctgggcga tgagctcgcg ttccttgtcc gtcagggacg
    36181 tggcgggggt gttcatgtcg gtggatgtgg cgttttcggt catgatgttc tccttatata
    36241 tacggtatat atgcgtggct gcgtatggcg tgtcacgcgg ttgcggatgt ggtggttgcg
    36301 gtaatgacac ccttcttcaa ggtgacggtg atgccatccc cagggcttac ctgcgaagca
    36361 tcgtctagta cgtggccatc ggcggactgc accaccgcat agcctcggtt caacgtggat
    36421 tgcgggctga gtgcggtcag tgaggcgtga gctcgctcga cggtgagctg ggcatcgtcc
    36481 acgatgcgtc gcagcccgat atcgagccgt tgcaacgagt cgtcaattaa gcgctgatga
    36541 ggctcaagca tggtgtgcgg ctgtgtcagg ctcgggcggt tcgcatatcc ctcgataagt
    36601 cgaatctcgt tctccacgcg cgaacggacc cgcagtcgca tacggtcgat ggcaccttcg
    36661 atgagctgcg actgctcacg cacatccggc accacgcgct tggccgcatc ggtaggggtg
    36721 gaggcgcgca gatcggcgac cagatccagc aatgtccagt catcctcatg cccgatggag
    36781 gagattaatg gtgtagtgca ggcatacgcc gcacgaacca cacgctcatc cgagaaaccg
    36841 atgagatcct caaacgagcc gccgccacgg gccacgatga tcacatccac gctcgggtcg
    36901 gcatcgagtt gtgcaatggc ctgaaccacc tcggccgggc attgctcgcc ctgaacatgc
    36961 acatggacca ccttgaaggt tacggaaggc caacgaaggt tcacattggt gatcacatcg
    37021 ccttccgcac gtgcctgagg tgcgcaaatc aggccaatag tcttcggaaa ctccgggaga
    37081 ggcagtttgt gatcggcatc gaacaggccc tcgcccttaa gctgcttgcg caattcgtcg
    37141 atcatggcct tgaggctgcc accggctccg accttgagaa tcgtgtcccc gcgcaacgac
    37201 agcgaagtgc gcttcatcca caggttcggt ttgccgtgaa tcaccacgcg gtcgccctga
    37261 acgaactggc tggctgcggc cgcgaacctg ccgaagccat tgacttccat ggcgatatcc
    37321 tcgaagtcgt cgcgcagcgt gatgtaggcc gagccggcgc gacgggtatt gatctgcgtg
    37381 atctggccgg tgacccacgc ggacggccat cgttccacgg caccgtagaa cttctggctc
    37441 agcacgctta ccggccacgg attttgcgcg gtggtttcag cggcgcgtgc aggcagctga
    37501 tcgatagggc gaggctcatt cggcagattg atggcgcccc gatcgggcgc gaaagtgcct
    37561 tgacccggct caaagccaga cgaggccagt gaagcggcgg tcggtggacg gccggtagat
    37621 gagtagccca ttgcagtagt catgcactcc actataagtg cgttggttga tgacggtaaa
    37681 aatttaccgt cgcctgaaaa cggtgtgatg atgaatgcaa gaaccgtacg aagcgatgta
    37741 tcagccgatg atggagatgg tctggtgcat cagaagaaag gagtcattat ggcgaagaaa
    37801 cgggtgctat gaagaggagt gagatatgcg ttgcactgac gattgagagt cgtcagtgag
    37861 ctcgctgtgc gacacgcgag cagaaaacag ttacgctaat cacacacttg tagttttggc
    37921 gaaaaatagc cgaaaaaccc tataaatagt tcgacacacc actgttgagc actagatata
    37981 ggggtgggga tgccgtattc ttctagaggt tcaagaaaac gatatcaatg taatttcgtg
    38041 tggcccacgt cgagtgcggc caaaatgcgg aactcaggag agtgttcgcc ttataggaaa
    38101 ggggtgcccg atgggcgcac aggtgctgga ggaaaccgtc accgataacg agaccaaggt
    38161 ggccgccaag gcctccaccg tgctcgttga gaagcgtgat ggtcgtgtgg tcgacttcga
    38221 ccccatcaac atcatctctg cggtcaagtc tgctttcggc gacctgaaca aggaagtcgg
    38281 ccccgaagaa gatgccatga tccgtggttt cgccaaccaa gtcgaaggcg aaatcaaggg
    38341 gcgttacacc ggccccgcca aaatcgagga catccagaat ctcgtggagc acgccctcat
    38401 tgacgcccac ctgtacgatg tggctcgcgc ctacaccaat taccgtctcg acaaagacat
    38461 tcagcgtgcc aaggccactg atgtcaacga ggccgttgct cgcttcatta atcatgaccc
    38521 gaccctgatt cacgagaacg cgaacaagga ttccaacgtc tactccaccc agcgtgatct
    38581 gctggccggt gccgtgtcca aggccgccgc gttcaacatg ctgccgccgg cggtgtccaa
    38641 cgcccacatg aagggcgaca ttcacttcca cgatgccgac tattcgccgt tcactgccca
    38701 gtccaactgc tccttgccga acttctggga ca
//
LOCUS       DAWBMW010000002        38231 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_342_length_38231_cov_3.391136, whole genome shotgun sequence.
ACCESSION   DAWBMW010000002 DAWBMW010000000
VERSION     DAWBMW010000002.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 38231)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 38231)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..38231
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_342_length_38231_cov_3.391136"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    11213..11222
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11815..11824
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    14207..14216
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    20246..20255
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    25611..25620
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    26295..26304
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    32511..32520
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         8011 a        10706 c        11385 g         8059 t
ORIGIN      
        1 gcgtccgaca acaggatcaa ggtcaccatc cgcatggcct acggcttcca ccacgtcgac
       61 aacctcaccg ccctgatcac gctccgatgc ggtggactcg acatccgact accacaaccc
      121 acgatctaac ccacgaaagc agcagaagcc tctaagaagt ccgctctctc cattagcacg
      181 cgagcaaaat ccaatctcat cgcgccccca agaacgaagt aatataccga ttgagcattg
      241 tgacaccgct atgagtctgc tcaaggagtt gtcggttgcc ttcaatgcgt ttctctaatc
      301 ctttaatggt ttgtccggat tgatcagcat ctgttccttt gataacaaca gcttgctcct
      361 ccgactcttg cttttcttca cgcctagcat ttacttcatc ggcaacctcg ctcagtaaat
      421 gattcttaag ctgtcgttta gcctcctgaa gcctcatggc aaaagattga gccacctatg
      481 cgatggattt gcgaactatc ttcaagaatc gtctaggcca aaatccggca ctctctatta
      541 tgggccatgg aaagaatagc tttggctctt caagtacttg tgtaagcaga atatctaatc
      601 ctgcttaccg gttattagcg tctgacctcg actggcttgt cggggcgggc gtcaccatcg
      661 cggcctataa cgtggacgaa ccatgtacgc ctctcgaaat atgcaagaaa cggaacttgt
      721 tcaaactttt ctacaacgat gtcagtctac tgaccgaatc gcccctacgc aaaacgcaaa
      781 ccgcgctcga tgtgctcggc ctgcgcacgc cagcacggag ggggcgaatc acctacttgc
      841 ccatctacat ggccggaccg ttcctcaacg aataattttc ccctagccgg tagaccacta
      901 cgccaatacc cctcagcatc ctcccagtga acgtttagtg aatttgttct gaactaatcg
      961 ctacaatatg cgggtacgtt gttgtgacga gggtacaggt gaatggcagt gagcagacag
     1021 tgcaaaacgc gcggcaatcg cacgaagacg ggaacctccc agctcggggt tcccgattct
     1081 tccgaggcgg cccatcattc tgcatcatcg gctaggtccc gtttcagtcc gcgagtcgga
     1141 ttgctcacgt ttgttgcttc tcggcgccgc gttcaccaac ccaaaacaga ttcagcagag
     1201 actactgtgc tcgctgacga aatcgaggat ttggatacca ctacacccga ttctgcggat
     1261 actgggattg ccgaaaccag cgctgattcg ctgaactctg catcggaagt cagcaatgac
     1321 gatatcgaca atgctcaggc tgaatcgaac gctaaagatt ccgacaccga agacaccacc
     1381 gcaaaagacc agagaagcaa aaagaccaat agccgaaagc ataagaaacc acagaaaccg
     1441 aataaaatca aagcatcgct tgcccgtttg aaccaattca tcaaagcaaa tgctgcgcct
     1501 attctgactt ccaaattcgc acaactcctg atttcagctg cggtagcgct ccacaaacta
     1561 tggaagaaac gcccaaagtg tccgtacacg ttgtatgtcg tagtgatggc gctgattgac
     1621 gcagcagccg taaccttcat tcagtggggc atgtattccg agccgaagta tgacgacccg
     1681 aacgcggtgg atagcactac caagattctc aacagcgtaa acggccaact caccagattc
     1741 gtgacgcaaa tgtggctaga agaagacaag ctcaactggc ttcttaattt cctagctctc
     1801 ggcatggtct acttggtgct ggtatttgtc attaaccggt tctgggtggc gaccgccgta
     1861 ttcgccatca cgatgagcgt gtacgccgta gcgaacagta tcaagataat actgcgcaac
     1921 gaaccaattc tcccctccga tctcagcttc ctgtccagcg gcaacggtgg cgaaatcact
     1981 tcgttcattc ccaaaagcag tcaggcattg gtagacggca ccatcaccat gctcatctgg
     2041 ctgacggtca tctgcctgat actgcaattc gtagacggac gccgttgtgt catcccattc
     2101 cactggtggc gtccattccg taacgtgaaa acgattatcg gcaatgtcac ccgcattatc
     2161 gcagcggcaa tgagcatcac gctgctgtgc tcgttcacat ggacgctcag tgttccagga
     2221 gcttggggat atgaatgggc aaagtcttgg ggtgattcac cgcaactctg gagtgccgaa
     2281 ggtgatgcag caaacaacgg tcctgtcatc aatttcttgc gccttacgca tccgaagatt
     2341 atggataagc ccgaggggta cagccaggaa acgatggaag agctggccaa gaaatacagc
     2401 tccgaggcaa accagattaa tggaacgcgt gctaataacc tcaccgataa caccgtcatc
     2461 atgattcttt cggagagctt ctccgaccca acacgcgtgc ccggcatcgc cttggccgaa
     2521 gacccgatgc ccaacatccg agccctgaaa gaaacaacca cctccggtct gatgctctcc
     2581 ccaggcttcg gcggaggcac cgcgaacatc gagtaccagt cgctcaccgg attggatctt
     2641 gccctgtttg acgattcgat gcaatccatg taccaggagc tcgtcccgca tcaaaagaac
     2701 ccattcgcgt ggaatcaaat ctggaatgcc gaatacggca aatccggttc cgtggcattc
     2761 cactcgtatt acaagaacat gtatctgcgt gacgcgaact acaagaagtt cgggttcaat
     2821 aagttctaca cactggacag cgaaccagca attacacacc aagacagaac cgacaattct
     2881 ccatatgtca atgatgcagc ctcataccag aacatcatcg accaactgaa caaagaggaa
     2941 caccctcagt tcctgcagct cgtcaccatg cagaatcaca tgacctatga caactggtac
     3001 ttcaacaacc agttcgaaca ggcgaatgtc accgaaaacc tgaatgatta cgaacgcgga
     3061 caaatcaata cctatgcaaa gggcgtgagc atcaccgatc aggcaacaat cgacttcctg
     3121 aaccagctga atgccatgga caaacccata accgtgattt tctacggtga ccatctgccc
     3181 agctcctatc agactgccgc agcagataaa aacaatacat tggcattgca tcaaaccgat
     3241 tacttcatct ggtcgaacca agcatccgcc tcagctggcg taaaactcga tgctagcaac
     3301 actgcataca cgtctccgaa ttacttcatg gagatggcag ccgaacacat gaacgccaag
     3361 gtttcgccgt atctcgcatt ccttactcag acacgaaccg atatccctgc actagaacgt
     3421 ctggtgattg gcgcaggcgg cttcgataca gacgcatcca cagcgtatct ggatcagaac
     3481 ggcaacgcaa tcaagcgtaa ggcactgtct aagcaggcca agaacacctt gcacgactat
     3541 aagctcatcc agtacgacat gactgccgga aagggatatc tgaacgacac caacttcttc
     3601 acagtgaagt aatgccaaac aagaatggcc atctgccgcg cattgtgcag gtggccattc
     3661 ttgtttgtgt ctcatttata tactggcatg tatggcacag actgcagttg atcacaaggc
     3721 tcaatgggaa acggtgaaca gggtcgttta ccccgttaaa gacactgatt tgacattgcc
     3781 cttgtatgca gtgaactggc atccttccca ttttgatgcg aacgtgttcg atgaacgcga
     3841 cagcatgatg agcgtcaatc tgaatgctgt aagccgttcg gattttcagt aatctcgtag
     3901 ctcatggaaa taggccactg gccgatgatc cgcacggctt cactgtcgat gcacgtaccg
     3961 tcatcacatt ggaaccgaac acgcatatct cgctatgcac ctacttcaat gctttcccag
     4021 cgggatattg gcggcactgg acgcgcgtta agcaactgcg ttttcttgcg gaggctacgg
     4081 gtaaggcggg aaaacactgc cgaacccgcc cggaaaatca ggagacgacg gcccgacatc
     4141 atcagaacca tccggcttgg atcctccaac tccaggctcg aggcgtccga caacaggatc
     4201 aaggtcacca tccgcatggc ctatggcttc caccacgtcg acaacctcat cgccctcgtc
     4261 atgctccgct gcggcggact cgacatacgc ctaccccaac caagaaccta acccacgaaa
     4321 acagcagaag cctctaacat atttgcttat ccaaggtatg agagatttgt ggtacctgtg
     4381 gctggaaaag gataatgagg gactccgaaa ggatgcttaa tggtacggga ggaaaaacgg
     4441 tatatcgatt caatcggctt gtcgtatgtg gacggatact gattatgcgc ttggggttgg
     4501 tgcgcgtatt gagtgtgtgg tcgaaccagc tgaagatgag tgggggtgtc attgttgacg
     4561 ttcaagaaag catggcagat accaggtaaa tgatttacct accttcatat tttgcggttt
     4621 gggctgacac taaaagatgg gggtgcgact cacggttgcg cccccatctt ttagtgcgtc
     4681 gctgtcagtg cctcttgttg tatgcgattt tgttggtgtc aatgacgaag gttttgcggt
     4741 cattcttggc catgacatcc aggataatgc cggcgatgat gagcagaaga ccagcgatga
     4801 ccagcatcac cgcgccgatc agcgtgggga agtgggtgac cacgccggtc ttggcgaatt
     4861 cgaaggtgac ggtgccgcag agcacaatgc cgacgatgcc gatgattagg cccaatccgc
     4921 cgaagaaggg gaggggcttg tattcgcgaa tcatgcggaa gatggtgctc atcaccttga
     4981 tgccgtcgcc tacggtgtcc agcttggaca ctgagccttc ggggcggtcg cggtactgga
     5041 ttggcatctc gtagagtcgc aggttgttgt tcagcgagtg gatggtcatc tccgtctcaa
     5101 cctcgaagcc tcgcgagagc acgggatagg tcttgacgaa ggtaaagtta aatgcgcggt
     5161 acccggtcat gatatcggtg acatgtgctc cgaacagccc gttgatggag ccgcgcacca
     5221 aacggttgcc gaaattgtgg aacggacgct tgttctcttg gaaataagtg gatgagaggc
     5281 ggtctccaat caccatgtcg tagccttcga gcaccttggc caccatggcg ggcgcggcgt
     5341 cggccgggta ggtgtcgtcg ccgtcggcca tcacgtacac gtccgcgtcg atgtcttcga
     5401 acatggcgcg gatgacgttg cccttgcctt gacgaggttc cttgcgcaca atagcgcctt
     5461 cggccgtggc gatttctgcg gtgcgatctg tggagttgtt gtcgtacacg tagatggttg
     5521 catccggcaa tgaggtcttg aaatcacgta ccactttgcc gatggtcact tcctcgttgt
     5581 aacacggaag cagcacggcg acagatggaa tcttctcttc aggcatgggc actactgtag
     5641 acgttaggat agtcttggaa agaatgctga gttgttctta atcagtagtt tgcataggat
     5701 aagaccaaag tgcgcaagat atcggggcac taccttgcat agtccgatat gctttgtgcg
     5761 actactcaga gtgacattag aggctggttg gaaaacatga actattcacg agaaacgaat
     5821 aaatacattt tgcctgtaaa tgtgcagaaa atcctaaagg ctattattaa gcctgaatgg
     5881 tgcttcctaa tattaatgct tatcatcggc agctgttttg ctgtattggt tccagctggt
     5941 ggtggagcag atgaacctaa ccacattgct cgcgcagtca gcgtagcgca tggtgatatg
     6001 ttcgctgata agattcctga ccgagtgggt tatacctctc ctcaggacgt cgatggtgtt
     6061 gattctgctt tatatggtgg tgtggttgat tcagccctca ctgatgtcgc ttggcacaat
     6121 atggttgatt tccatactcc aaaaaataaa ggagggaaaa tatactcatt tcctacgtgg
     6181 accactcaag gaatgatgag tcatcgaaac gtaggcgaga aagaacacgt cgaggcattt
     6241 tctaattctg ctgttaattc tccattcgtg tatgcgccgt tcgctttggg atactggctc
     6301 tcgcgaattt tcaccagtaa cgcatacgca atcattgtaa ttatgagaat gtttgggttg
     6361 ctgactgctg cgggcatcct gttcttctgc attcgatata ttcctattgg gaaatgggtg
     6421 cttgtttctg tctcgcttat gccgacgatg gttatttttg atgctacgat tacggctgac
     6481 acgatgacat atgccatgtg catatcgttc attactgtgg tatttaaatg catgtgtgaa
     6541 aatcgtgcgt tgcaccacaa ccagtggatt ttgctttcat tggtttcaat atctttggca
     6601 ctgatcaagc tctcctatgt cccgctcatc attctgttgg cgttgattcc cattgccaat
     6661 caatatgtgc gcagcaagaa ggacttattt tctttgctag ggattatgct ttgcacggga
     6721 atcttgttta tcggttggta tctcgctgtg tcgtctatca atactggtgc gatgtttgat
     6781 gttggagctt ctccgacttt gcagaagcaa tatgtattca gccatttgat tacgtatatg
     6841 ggactccttg tcaagcaatt tataggacag aatttcttca acttgggcgt taatggtacg
     6901 cttgatctac atggacatta tgcttttacc ggttggatta ctgtttgtgt attaatctgt
     6961 gcagtgttgc tcatcgattc gagagagaat cgtctggcta ttttcaggaa aaatgcggtt
     7021 tggtgttgtt tatttttcct gcttgcagca gcatgcgtat tcgggcttgt ggagactgca
     7081 ttgtatctac agttttctcc cgttggagca gacgatattg ctggtgtcca accacgatat
     7141 ttcctaccga ttttaccgtt gcttattgtt gttgtggtga ttatgacaag acagacagat
     7201 gaaagtaccg ttcagtctga tttagagggc actagaggta ctcaagtgct ccgtgaagcg
     7261 gaaaactgtt tgggtattgg tatgatgctt agcctgcagc tcatctccgc aatattcacg
     7321 ctgtatctat tattcacgac gctgtatact gcttaaggtt gaggtactca catgtcccgg
     7381 tgtctatatc gtttgtccac aatatactag gtgtttatgg ctgaagaact attctccccg
     7441 aagaacatca ttgtcaccgg tggctgtggc ttcatcggtt cgaacttcgt gcactatgtg
     7501 tacaacaacc atccggacgt gcatgtgacg gtgttggatg cgttgacgta tgcgggcaac
     7561 ttggagaaca tccgtccgat cctgggcgat cgtgtggagt tcgtgcatgg gaacatctgc
     7621 gacgcggagc tgttggatcg tctggtgccg gggcatgacg cgattgtgca ttacgcggcg
     7681 gagtcgcata acgacaattc gatcgcgaat ccggagccgt tcctgaagac gaacgtggag
     7741 ggcacgttcc gcctgttgga ggcggcgcgc aagtacggcg tgcgctacca tcacgtgagc
     7801 acggacgagg tgtacggcga cctggccctg gacgatccgg ccaagttcac ggaggagacc
     7861 ccgtaccatc cgtcgagccc gtacagctcg acgaaggcga gctcggacct gctggtgcgc
     7921 gcgtggcacc gcacgttcgg ggtgcgcgcg acgatctcga actgctcgaa caactacggc
     7981 ccgtaccagc atgtggagaa gttcatcccg cgtcagatca cgaacatcct gaatggtctg
     8041 cgtccgaagc tgtacggcga cggcaggaac gtgcgcgact ggatccacac ggacgaccat
     8101 tcgacgggcg tgtgggcgat cctgacgaag ggccgtatcg gcgagacgta tctgatcggc
     8161 gcggacggtg aacggaacaa cctgacggtg ctgcgcgaca tcctgaccgt gatgggcaag
     8221 tcacccgatg atttcgattg ggtgacggac cgtccgggtc atgaccgccg ttacgcgatc
     8281 gattcgacga agctcaggac cgaactgggt tgggagccga cgcatacgga cttccagaag
     8341 ggcctggagc gcacgatcga gtggtatacg gagaaccgcg cgtggtggga gcccgcgaag
     8401 gccgccaccg aagccaagta caagcagcag ggacagtgat cgggtatggc gtttgatttc
     8461 gagaaggact tgaaggtcga ggagacgaac atcccggggc ttttggtgtt cgatctgccg
     8521 gttcatggcg ataaccgcgg ctggttcaag gagaactggc agcgggcgaa gatgaccgcg
     8581 ttgggcctgc cggatttcgg tccggtgcag aacaacatct cgttcaacgc gacgaagggc
     8641 gtgacgcgcg gcatccatgc ggagccgtgg gacaagtaca tctcgatcgc ggccggcgcg
     8701 atcttcggcg cgtgggtgga cctgcgtccg ggcaaaagct tcggtcaggt gtacacgacg
     8761 cgtcttgacc cgtcgaaggc gatctacgtg ccgcgcggcg tgggcaatag cttccaggcg
     8821 ctcgaggatg ggaccgtgta cacgtatctg gtgaacgcgc actggtcgtt ggagcagaag
     8881 aagacgtaca cgttcgtgaa cctcgctgat ccggagctgg ccatccagtg gccgatcccg
     8941 ttggaggagt cggaacgtag cgaggcggac ctgcatcatc cgatgctcag ggacgcgaag
     9001 ccgatggcgc cgaagcgcac gttggtgacg ggctgcaacg gccagctggg tcacgcgatt
     9061 cgcgcctacg cggaagccca tggtcttgag ggcttcgagt acacggatat cgacgagttc
     9121 gacttctccg atccggccgc gtacgacaag tacgactggt cgctgtacgg gacgatcatc
     9181 aacgcgggtg ccttcacggc ggtcgacaag gcggagacgc cggagggccg tccgggcgcg
     9241 tggaaggcga acgcgcaggg gccggccctg ctcgccaagg tcgcgaagga ccatcacatc
     9301 acgctggtgc atgtgagttc cgactacgtg ttcgacggca ccgcggacct gcactcggag
     9361 gatgaggcgt tcgcgccgtt gggcgtgtac gggcagacga aggctgcggg cgacatcgcg
     9421 gtgacgaacg cgccggagca ctacatcctg cgttccagct gggtgatcgg cgagggccgc
     9481 aacttcgtga agacgatgat ggcgctgtcg aaccgcgtcg ccgacccgga cgaccagctg
     9541 ggccaagtga cggtggtgga cgaccagtac gggcgcctca cgttcacgaa ggacatggcc
     9601 gaggcgatct tccacctgct ggactcgcat gccccgtacg gtacgtacaa cctgaccggt
     9661 tccggcgcgg tgaggaactg ggcgcagatc gcgtcccgcg tgttcgaact gacgaacggc
     9721 aacggggaga aggtcaagcc gatcagcacg gccgagtact tcgcgaacgc gaaggcaccg
     9781 gtcagcccgc gtcccgagca ttccgcgctc gacctgtcca agatcaaggc cgccggctac
     9841 acgcccgccg actgggagga aaccctcgaa gcatacgtga gcaaggaact cggcaagtag
     9901 gacagatagg acaaaccgga aaggtctgtc agcccattcc gacggcctga tgtcagtgtg
     9961 aaaaccgcgc aggatgacct gcgcggtttt ctgtttcgcc actgtcttgg ctaagctggt
    10021 gtgagctgat tacgactgtg aagaaagcag gtccttatga aaggcatcat tctcgccggt
    10081 ggctcgggta cgaggctgta tccgttgacg acggtgacat cgaagcagct gctcccggtg
    10141 tacgacaagc cgatgatcta ctatccgctg agcgtgctca tgatggccgg catccgtgac
    10201 atccttatca tctccactcc gaaggacctg ccgaacttcg agcgactgct tggcgacggc
    10261 agccagtatg gcgtgaaatt ctcctatgtg gtgcagccgt ccccggatgg tctggcacag
    10321 gcgttcctgt tgggcgagga gttcatcgcg ggtgagccgt gtgcgctcgt gctgggcgac
    10381 aacatcttct acggcaacgg tctgggcgtc acgttgcgca aggcgaaggc gaaggcggag
    10441 aacggtgagg gcgcgtccgt gttcggctac tacgtggacg atccggagcg ttacggcgtg
    10501 gtcgagttcg acgcggacaa gaaggccgtg tccatcgagg agaaacccaa gcatccgaag
    10561 tcgaactacg cggtgacggg cctgtacttt tatgacgagc gcgtggtgga gttcgccaag
    10621 caggtgaaac cgtccgcgcg aggcgagttg gagatcaccg acctgaacaa gatgtacctg
    10681 gaggcgggcg cgttgaacgt gcgcacgttg ggccgcggct acgcgtggct ggacacgggc
    10741 acgatggact ccctgtacga ggcgggcgag ttcgtgcgca ccgtgcagcg cgcgcagggc
    10801 ctgcccatcg ccgtggccga ggaaatcgcc tatgagaacg gctggattgc ccgcgagcag
    10861 ctgcttgagg ccgccgagaa gtacggcaag agcccgtacg gcaagcatct caaggatgtg
    10921 gccgacgaca agatcatctt gaaacagaag aatgcccgag gtgcctcatc tgaaaatgag
    10981 cgcgaagccc ggagtggtgc ctcaccggtg gtgagcgcga aatcctagtc ctcgtcggct
    11041 tgcggggttt cgcccccgtt gtctatttag aagtgcaacg tccttgttgt ttggttaatg
    11101 atggcagttc gttggcgaat gcctcggccg gggtgtggta gccgagcacg cacatgggac
    11161 ggttgttgat ctcgtccacg atggcctgta tctcgtcggc catggacggg tcnnnnnnnn
    11221 nncgtgcgtt tgggcaggtg gcggcggatc gcgccgttgc ggttctcgtt gccgacgcgc
    11281 cggtacgacg agtacgggtc ggcgaagtag gtggccatgc cgagccgttc gcgcagttcc
    11341 gcgtggcggg cggactcggt gccgtcgtcg tgcgtgacgc cggtcccggc cccagccggc
    11401 agcgcggaga acatgaggaa ccgcgctccg acgctttcgc gggcggtctt gtcgggcacg
    11461 atgcgcgcca taaggaaacg cgtgcgcctt tcgacctcgg tgtgcgggct gcatcccacg
    11521 ccgatgacgc cgtcggcctc ccactcgccg aaccgggaac ggtcggatgg tccgtagggc
    11581 ctctcgtgga tggatgtcct gagcggtatg cggctcctgg ccacgccccg gcccttcgcc
    11641 ctgcgcctgc ggcgatggcc cctcggcaga tagcgtgtcc agcgttcgcg gcgcgccttg
    11701 tcggcgtaga cccaccggta tatggtctcc gggcagcggt gcatcgattc gtcgccgggg
    11761 tgctccggcc tcagccggcc gctgaccggc agcggcgacc agcccctgcg cagcnnnnnn
    11821 nnnnggattc gttctcgttg gacgggaacc atgtgtcgcg tttgatctcg cggctgaccg
    11881 tggaggcgtg tctgccgatc aggccggcga tccgccgtat gctcgtcccg ttgccgatct
    11941 ctatctggat gatctgccgt tcctcttccg acaggtgcga atgcacgtga cccaaggtgc
    12001 gacacccttt cactggtcat ctggacaatc tccaatttaa agggtgttgc acttccatct
    12061 agacaacggg ggggcaagcg tcgttcggca gggctgctag tagggtaata ctactatgac
    12121 aatgcttcag gcctgttgca cgaggaggaa tgttgaagct gttcagtaag aggaaccgtg
    12181 tgctgctgca tgagctgacg gtcaccgatt tcaagctgcg ctaccagcag tcggcgcttg
    12241 gctacctgtg gtcgattctc aagccgctca tgttgttcgc ggtgatgtac atcgtgttcg
    12301 ccaagttcct ggcgatgggt ggagacattc cgaactatgc cgtctacttg ttggccggca
    12361 tctcgttctg gaccttcttc gccgaagcga ctacgcaagg tctgcaggcg atcgtatcgc
    12421 gtggcgacct catccgtaag atcaacttcc ccaagtacat catcgttctt tccggaacga
    12481 tctccgcgct catcaacctg ttcttcaact tgctggtggt gcttgtgttc gctttgctga
    12541 ccggagtgaa gttccgttgg acagtcctgt tgctgccgct gaacattctt gaaatgtacc
    12601 tgctggcgtt gggcattggt ctgatgctgg gatcgctgtt cgtcaaatat cgtgacgtcg
    12661 gctacctctg ggaagtcgct ctgcagctct tcttctacgg tacgcccatc atttacccga
    12721 ttacgatggt gagtgctacg catccgttta tggcgaagct gatgatgctg agtcctatgg
    12781 cgcaggtcat tcaggatatc cgatacaact tggttacgga ctacacgcag accttgtggt
    12841 ccatgtgggg gactccgctg atcggcttgg tcccgctagg tatcgtgctg gtgattttgg
    12901 cgttcggagc ttggtacttc ggaaggaact ccaagaagtt tgctgaggtg atgtgacgat
    12961 gtctgattcc aagaagacca aagaagttgc cattcaggtt tcccatgtct ccaaaagctt
    13021 caaactgcct ttggagaagg cttccagtct gaagcaggcc atcttcaata cgttaagcgg
    13081 taagaaggga tacgaggaac atcaggttct ccgggacatc accttcacca tcaacaaggg
    13141 cgatttcatc ggtatcgtcg gtaggaacgg tagcggtaag agcacgctgc tgaagctgat
    13201 ggctcagatt tattacccgg agacaggcga tatcactgtt aacggtgtcc tcgtgccttt
    13261 catcgagctg ggtgtcggtt tcagtcccga actcacagga catgacaacg tgtaccttaa
    13321 tggcgcgttg atgggattct ccaccaaaga aatcgatgcg atgtacgacg acatcgtcga
    13381 tttcgctgaa cttggcgagt tcatggagca gaaaatcaag aactactcca gcggcatgct
    13441 ggtgcgtttg gccttctcca tggcgattcg tgcacagtcg gacatccttc tcctcgacga
    13501 gattcttgcc gtcggtgacg aagcgttcca gcgtaagtgt tatgcatact tcgccaaact
    13561 ccgccgtgag aagaagacgg ttattctagt gacgcattcc atggactccg tgcagcgttt
    13621 ctgcaatcgc gcgattctga ttcacgacgg cgacctgaag atcgatggtt ccccgctgga
    13681 agtcgcccag gcatatcagg aactcaacag ccaacgcttg gatctggcta gcttggaaga
    13741 gcagttcgcc ggcgcgagcg ccgatgccga acgcaagcaa gagcaggaag aggccgcgcg
    13801 ccagcgccga gagaacgtgg atgtcgctgt aactcacagc gtgagccagg acaagctgac
    13861 cttcgatatc gatctcgagc cgaagcgcac tttggaggat gccgtcttca cgttcgtcat
    13921 caacagtgaa gtcggcgaga ttgtgttccg tgtggcctca gacgacaaga acgtcaatca
    13981 gaccattgac ttcaccgaag ggcataagtc gcatatccac ttggagatag acaacatctt
    14041 ccccaacggc atcttctcgg tgcaggtcgc cgttaagaag tccgatcgta gcgacaacta
    14101 ccttatggcc aataatgtgc tgctcttcga ggtgcacgct cccgttgatg tcagccagaa
    14161 ctggaagcct ccgttcgatt tcatcgtcac ctcgcagaaa ggttaannnn nnnnnnttaa
    14221 tgatgcagta cggccttttc tcatacgaga ccgaaaatat cggtgatgag attcagagca
    14281 tcgccgccag acgtttcctc ccggcagtgg acacgtacgt agatcgtgac tatgtcggac
    14341 aatgggaacc gcagggcgat acgaaaatca tcatgaacgg atggtttatg catcccccgt
    14401 acgcatggcc gccgaaaagc gagtcgattc atccgctgct ggtctccatg tatgtggatc
    14461 aggccgattc acgagttggc gaagcgttct tctcttctga aagcgtggcg tacctcaaca
    14521 gacatggacc tgtcggggca agggatatgt ccacgttgga gtttctgcgc aagcacgggg
    14581 tcgaatcata tttctcgggg tgcatgaccc tgacacttca agctgacccg acgattgaga
    14641 agcaggacta cattcttgct gtcgatcttc ctgaagccgt ggtcgaaatg ctgcgtcaga
    14701 agacgaagcg tcctgtaatc gagtcctctc cctacttcga cgcgaacatg agccgggaaa
    14761 accggttcca ggtggccgag tacttcctgt tcctctacca gtcagcgcat gcggtcgtga
    14821 ccacacgtct gcatgccatg ctgccgtctc tcgccctagg aactccagtg ttccttgtca
    14881 tggatcatct gaagtacgat tcccgcaggt atgcaggtct ggatgatttg gtgaacaaag
    14941 caacggatgt tgattatttg aacgactata atttgttcga tgttgacaac cccccggcta
    15001 atccggataa gtatctgtcg attcgtgaaa agctgattgc tgatgtttct gcctttaccg
    15061 gatacaacaa tgataaaacg ttccgcacag tcgatttctc acgtatcgcg atgaacgaag
    15121 cttatgtgcg tcttttcacg cgaggcttcc attccctgaa tagggcgttg ctgttggctg
    15181 gggataaagc ctggctgcag gagaaatacg acgatttgta tatccattac gcggacctgc
    15241 aaaagaacta tgaacagctg gttgctgtct gtaaccagca gaaccaagag aaagagcggc
    15301 tgtcggctca gctcgatgcg gtttatcatt ccgggacttg gaaagccggt agggcagtgt
    15361 ccgcgcctgt tcgtctgatt aagagaatta tcgggaagtg attatgagat acattgcggc
    15421 attcttccag tacgttcgcc ataacggtct gaaaacagct attggtcgta cccttggctt
    15481 catcaagcgt aagatcaagc gtgcgcctgt tgttcagaac gttgtggtta agcgtcccat
    15541 tgacgtgatg acatccgcgg aagatgttgt cgcggctgat tttgtcgctc atccgtataa
    15601 gacaccagcg aaggtgaaca aggccaagct caatatcgga tgggttctgt ctcccatcag
    15661 tgtcggtagc ggcggtcagc ataccattgc acgctttgct aagtccttgc aagaacgcgg
    15721 tcataatgtt acgttcttca tttacgaaag cattgcgcag cagccggcgg aatactcaca
    15781 taaattgctt aaggatcatt tcgacatcga cgtgaagtgc gtgccagtca aggacattga
    15841 caagagtgat ttggatgttc tcttcgctac tggttgggaa accgcgtacc cggtgttcaa
    15901 tatgaagacc gaggcccata agatgtattt tgtgcaggat tatgagccgt tgttctatgg
    15961 tatgggttcc aagtccgtgc tcgccgagaa cacttaccga ttcggtttct acggtgtcac
    16021 tgctggacgt tggcttacca agaaggtagg cgagttcagt atgcccgctg actactttga
    16081 ttttggcgct gaccttgata tctataaacc gaagaccaat gagcctaaga aaaagcagat
    16141 ctgtttctac ggccgtcctg tgacggaacg tagggctttt gaaatcggtg tgcttgcgtt
    16201 ggcaaagttc caccagatgc accccgacta caacattgtg ttctttggtt gggatgtgtc
    16261 ggacttcgat atcccgttcc cctacgagaa ccgaggtatc gtgagtccgg ctcagctcgc
    16321 ggaaatctac catgagtctc aagcgtgtct ggtgctttcc ctcaccaatg cgtccttgct
    16381 tccgttggag ttgttggcgg ctggatgtgt accggtgatg aatgacggcg acaacaaccg
    16441 catggtcatc ggagagaacc cggacatcgt gtacacgctc aattctccga tcgaacttgc
    16501 acatggcctt gatgaggtcg tgcgagatcc ggatatcgaa gcgcgttgtc agcgcatctc
    16561 caagcggacc gaaagctctt cttgggatgc atcctacgat aagttcgaac ggattatcct
    16621 acgagaggcc actacgcaga atcatgactg acaacatcaa agccaccgta tttatcccca
    16681 cctattatgg tgaggacaat ctggacgagc tgttcaccgc attgtacaag cagaaggtcg
    16741 atttcgagta tgagattctc gtcatcgaca cgagctctaa ggatcgcaca cctgagatta
    16801 ttcgatcttg ggcgcccaag ttccagcatt tccggtatga gaccatcact aaacctgaat
    16861 tctcccacgg gcgagtccgt caacaggcgg cgaagatggc tcagggtgag tacgtcgtct
    16921 accttacaca ggatgcaacg ccggctcatg accggtggct ttatgaaatc attcaaccaa
    16981 tggagcgcat tcctcaagtc gctggtgtga tgggcaagca ggatccgcgc cccgctgcat
    17041 tgccgttgct gaaagaggaa atcatccgaa cgttcgcgca gttcgggcca gactttggta
    17101 cgtcagtgtt ctatgaagat gaattcatcg attcgcagca ggttttcgat cttgtaacgt
    17161 tctactctga cgtgaattcg gctgcacgaa aatccgtatt gctgggcgat gtaccttatc
    17221 aggatattcc ctatgctgaa gaccagaaat tcggtgagga cctagtgcgt cacgggtata
    17281 tgaaagcgta tgctccgcgc gcaaacgttt ggcacactaa cgaaataaag ctgcgcgatt
    17341 ataagaagcg catgttcgat gaaattgttg gtgtgcgtcg tgtgggtaag accatggacc
    17401 cgatttccat cccgttcgtc atcaagcagg tcgttcgtgc gctcactttg ggtagccgtt
    17461 tcatcctgcg tgacggacag tattcatgga agcgcaagtt gtactggctc gtggtcaatc
    17521 cattgttcgt cgtagagaag tggaaaggcg tacgtgctgc catcgccgtc gatctgaagg
    17581 atgagcaggc gattgcaaac cagtcgttgg agagcagcga gcagcagcgg cacgagagtt
    17641 gaaccgcgat gccgcatagg tgaggtatgt gtatgttctt gcatatactc tagcgcgtat
    17701 cctatggaaa taagcaataa tttagggctc ttcgggtgtt tgtgtgggtg tggattattg
    17761 gtctggctta cgggttattg gcgtccgggg agtcggaggt tgagtccgcc gcatttgagc
    17821 atgatcaggg cgatgaggtt gccgaggttc ctgtagccgt agccgatctt gatggtggtc
    17881 ttgatcttgt tgttgacggc ctccaagcga gcgttggata gtccggctcg ggtggtgcgc
    17941 aggatgtcga agtggcgtcg gtcgatcttc tcgccgagcc gggcgaattc cgcgatgccc
    18001 gagagggacg cgtccgcggc ccactggcgc agcatgggcg cgcggtgacg acgtccccgc
    18061 catgcgggtc cttctcgacg cgcggcacgc cggtcacgac gttcgcgggc agattgagca
    18121 tacgcttgaa cgcgccggcc gatatggcgg gcaaaggcgt tccctctgtt ttccgtttgt
    18181 gttcctgacg gaaacaaacc taacaggaga acgccccttc catacccgca cacgaaaaag
    18241 ccccggcaac ggacgccggg gctttcccgc cacacaaact ggcgaagact ctaaagaatg
    18301 tttatcgcat atttttgatt ctgcggtcgt agatatactt ctcaaacaga cgaatgcgct
    18361 ggatgacggc gcaccgcatg cgctgtgcgc gggtgagatt ggctaggaga gtatcaattt
    18421 cctgacggtc ggtttcgtac tgctgctggt tttctggaat atggaagtca ggagcttccc
    18481 tctcggcttc cagttcgccg atcaacgtgt agtagctacg catggggagg acgcctcggt
    18541 caagacagag acggaaatta tctgatgccg cagtgatgtg gtcgtggtgg aacgcgaagt
    18601 cacgcttttg gtgtgttacg gattccgggc gctgaagcca gttgtacagg ttcacgtcaa
    18661 tatccgccac tttgttcata cgcacgtaca ggtgtcctgc gatagctgtg tcctgcgcgt
    18721 acatgccttc cgggaatcgc acgccgtccc atagttcgcg cttgtacagg cggcaccagt
    18781 tcgcctcgtt gaaatagcgg gcctccgtgt tgtgtccaag cttgtcgccg attaggcgca
    18841 ggctcttgca gaatacggtc tggtaggcat tcagcggatt cgtgaacacg ctcgcatgtt
    18901 cgggttcggc ggcgccttca cgggcttcca tcgcgacttc ctcaatctgg gagacgccga
    18961 actgcctcca gcgggccttg ctcatgtccg cgccggtcga aatcagcgcg tgcagcagca
    19021 gttcgatgtt gcggcggtcg aggatgtcgt cgttgtcggc gaacgcgatg taatcgccgt
    19081 gcgccgcatc caatccggcg ttctgcgcct tcgcgatgcc accattcgtc tggtggataa
    19141 cgcgcacgcg attgtctcgt cgctggtatt cgtccagaat catcgggctg gaatcggtgg
    19201 cgccgtcgtc cacgaggatg atttccagat gctcgtacga ctgccgaaga atcgaatcca
    19261 cgcagtaacg caggtatttc tcggcgttgt acaccggcac gatgatcgag acaagcggcg
    19321 aaacctgcgt catggcgaat ccttctaacg tgtctgcgcc ctgcggccgt aaccggcagg
    19381 ccgccctgtt tatgcggaca tgaacagtat gtcacagggt aggcgcacgt cgcgcgtgca
    19441 ccaagtaaag ccgaaaccca gcctgcgcaa gccggaagcc cgaagcctat aggagccgct
    19501 gaattcggaa tcgcatgtgt acataggtgc ttctcgctac actggtggac aatgaaacgc
    19561 gtgtggaatc gcacatgccg gttgaaaggt tttgagtcat ggcaaatgcg gcggagaata
    19621 cgcagcacgg cccgtcggag ggcgcgcagt acccggattt ggtgattgtc ggtgcgggcc
    19681 tgttcggcct gacggtggcg cagcaggcgg tggagcacac cggcgcccgc gtgcacatca
    19741 tcgatatccg cgatcacatc ggcggcaacg cctactcgta tatggacgag gagaccggcg
    19801 ccgaaatcca caagtatggc gcgcatctgt tccacacctc caacaagcgc gtctgggact
    19861 acgtgaaccg tttcacgagc ttcacgaact acgtgcaccg cgtgtacgcc acccatgacg
    19921 gcgaggtcta cccgctgccc atcaacctgg gcaccatcaa ccagttcttc catgcgcatt
    19981 acacgccggc cgaagccaag gccctgattg aacagcaggc gggcgagctg gccggcaccg
    20041 atccggccaa cctgaacgac aaaggcatcc agctcatcgg ccgcccgctg tacgaggcgt
    20101 tcatcaagaa ctacaccggc aagcagtggc agaccgaccc gtccgagctg ccggccgcca
    20161 tcatcaagcg cctgcccgtg cgcttcaact acgacaaccg ctacttcaag gacacgtggg
    20221 agggcctgcc ggccgacggc tacacnnnnn nnnnncggct acaccgcgtg gatggagaag
    20281 atgatcgacg acccgcgcat caccgtggag ctcggcgtcg acttcttcga cgaatcccag
    20341 ccgtacaaca agaccgcgct caaggccgcg ggcgtgccgg tggtgtacac gggcccggtg
    20401 gaccgttact tcgactatga gctcggcgac ctcaagtggc gcacggtcga cttcaaggaa
    20461 gtacgctacg acgaaggcga ccacttcggc tgcccggtga tgaacttctc cgacgcggac
    20521 gtaccctaca cccgcgccat cgagttcaag aacttcaacc cggagcgcca cgatgcccag
    20581 aacccgaaca agaccgtggt gtgggaggaa tacagccgtt tcgccgagcg tggcgacgag
    20641 ccgtactacc cggtcaacac ggatgcggac aaggcactgt acgcccagta cgaggccaag
    20701 gccaaggccg agccgaacac cgtgttcggc ggtcgcctgg gcacgtacaa gtactacgac
    20761 atgcacaacg tgatcgacac cgccctgacc gcttacgagg agcaggtcgc accgctgctg
    20821 aagaagtaga tcgtcgtttc gccagaggat taacgattga gcctgaaacc gactgaatat
    20881 tgctgaggca atagccggtt tcaggctttt gtgtttttgg aagccaaatt tcctggggta
    20941 cttaaggctc gccacgagga gtacagttgt ctgtaacgca caatgtggcg cgtaagacga
    21001 ggggcgcatc tcgcaagtgg ggtgcgatgc agttgaagga gtgcatgtca tgactgatcc
    21061 gaatgtcaat ccgaacggcc aggtgccgtg gcagcaagct gacgccaatc cgactgagcc
    21121 gttaaatgcg atgacggcgg aaacgggaac cgccgccaac aatgctccaa cccaagcaat
    21181 cgcagcgggc gagcagccca ctcaagcgtt cactcctgtt gagcagcctt cgcaggcgtt
    21241 tacacctgcc gagcagccca cccagccttt tgcgccggtc aatcagccgg taccgcctac
    21301 gcaggctttg ccaccagtga ctcctgccgc tgcgcccgcg cccgcaccgg cagcacccaa
    21361 ttcgccctat ccgggggcgg ctccggcagg cggatcggtt cctccggtct acggcagcgt
    21421 cagtgctccg gtatatccgg gcggcggggc cgtgcctccg caaacgccct ataacccgta
    21481 tatgcaaggc ggccaaccga acggccaacc gggatggtct ggttcttacg gcagtgtgcc
    21541 gcctgaagcg aaccagaagt acaacggcct ggctattgcc ggttttatct gctcgttcct
    21601 tgtctcactc ctcggtctga tcctgagcat cgtgggcctc aaccagatta agaaacaggg
    21661 cggcaagggc aagggcttgg ccatcgccgg catcatcatc agtgcggcgg gcatggtgat
    21721 acaggtaata ctggtcatcg cgctgatcgt cggcggtgcc tcgtatgcca gcaaggctat
    21781 agatgaggcg aaaaccgatt ccttctatag tcagtcctct tcgggcaacg gtgatagcag
    21841 cgataagagc aaagctcaag ataatcttga cgatgcgctg gacgacacgt tgggcgaaac
    21901 ccatgacgat ctaaccgacg gcgattatgg gctgtacgat tcgattcagg atttcgtcga
    21961 ctctttggaa ttcaaggact ccattgaatc cgaagcggat acgtttgcag acaccggcat
    22021 caccttcaac taccgtgtcg acggtgacac cttggtgtac gagtacgtgc tcgacgacag
    22081 ctatgcctcg ctaggcgata ccgccgcttc ctcactggat gctatggaca gcacctatca
    22141 atccaccgcg aatctgctgg gctcgatgtg caagacctcc agcggcaagg catcattgcg
    22201 cgtcatcatg cacacgcaaa gcggccagtc gctgtatgac aaaacctgga ccgaaaagta
    22261 gcaatcccag cgaacgtgcg ttttggtcga ttgaaggccg gtctgcttgc tccttggacg
    22321 gatgcttctg cggaaggaac cgccaaggag taggcttaca ggcatgagac tcgcacgttt
    22381 ttctttgaat gattcgccgc gctacgcctt cgtgcagaag gacgaaacgg acggcaagga
    22441 ctacctcatc gaactggacg gccatccgct ggccggcgac caggtcaagc ccaccggcac
    22501 tcgttacccg gtcgacggcg atggcatccg tctgctctcc ccggtcatcc cctccaaggt
    22561 ttacggtctg gccaagaact atgaggccca cgcccagttc atgcacgagg ccggccactc
    22621 cgaaatcgcc cacgcgccgg aagacatggt gatcttcacc aagccgtcca cctcggtaat
    22681 cggccccgac gacccgattg tgtacccggc ctgctccaag gacatgaact tcgagccgga
    22741 agtggccgtg gtgatgggcc gtatcgccaa gaacgtgacc gtcgagaacg cgatggatta
    22801 cgtgctcggc ttcacctgcg tcaatgatgt gacgttgcgc gatctgcagg gcatcgaccc
    22861 cacgtggacg cgcgccaagg gcttcgatac ctcctgtccg cttggcccgt ggatcgtgat
    22921 gcgtgacgac ctcgactgga aggacgccaa gatttccttc acgctgaacg gcgaggatgt
    22981 gccgatggcg tctggcacca ccgcgaacct gattcacggc atccccgagc agattgccgc
    23041 catttcgtcg ttctccacgc tgctgcctgg cgacgtcatc atgaccggca ccccgaacgc
    23101 ctccggccac ctcgacccag gcgacgagac catcgtgcac gtcgaaggca tcggtgatct
    23161 gcgcaacgta atcgttcggg cgtgactcgt tctggctccc ctctctgagg ggaaccgtcc
    23221 aggcggagaa gctgactgag ggaaccaaga acgcgcctta cgctcccggc agaagtgcgt
    23281 gcagggcggt catcaccacc aggctgatga tggccgtcac tgtcagcgag aagccggcga
    23341 gtttggcgtt gccggtcagc gaatcggtga acttagttga gaaaatcgtg atcggggcga
    23401 aggagcccag cacgatgatc tcgcgaatcc tcatgtcaaa ctgccgaccg cgccgagacg
    23461 ccgggacagt tccgtgccgg caagccgcag cttggtctta agcgaccgct cctgttcggg
    23521 gcgcagcgat ccgctcacga aagtgagagc cagcccggcg atgggaaagt cgttgtagtc
    23581 gcgcacagcg acggcgtatg aatcaaagcc gggcgtcacg tctccgtgtt cctcggcaaa
    23641 accggtgcgt tgggtttcgg cgagcgttgc ctccagctcc ttgggcgatt tggggccgat
    23701 gccggtgcgg tcggtgaacg cctgacgatt gggatatagc gcgcggacct gattgcgggg
    23761 gagtgcggac agaatggccc gcccggtggc ggtcaggtga ctgggtaggc gcacgcccac
    23821 gcctgacact agcggcgggc gatgcgcggc gcgctcctca atcacataaa tgatttcgtt
    23881 gccgtgcagc acggccagat gtccgttttc gccggtctcg tcaacaagac gcgccaacgc
    23941 cggatgcgcg acctgcgata cacgatgttg gcgagaatag gccgatccca gctcgaaagt
    24001 cttgagtccg atgccgtacg cgcgctgctc ggcaaggcgg gtgaccagtc cgtgacgctc
    24061 caaaatctcc aacaattggt acaccgtgga gcgcggaatg ccgagctcgc gagcgagcgt
    24121 ggcggcttgc gtctgaccat tgctcgcgat gaactcgagg atgtcgagcg tacggtcggc
    24181 cgccggagcc agcgagacac gtggcgcgga tgatgatgtc tgatgttccc gttctgatgg
    24241 cttcacctgc gcccgcttga cggtacgtgg ctcggtgaag gcgtattgct gcgaggcatg
    24301 gctcatattt ccatcacctt acatgtctga gatttcagac acaagatgga cgaagtggct
    24361 tatggcgggc atccgactga ggtgaaatgg ccaaggtttg attactcggg gagcgattgg
    24421 tcgcgtttgc gtcgcctgct gttgatgggc aggtgcggtg agcgaatgcg ggcagtcgcg
    24481 gatccccggc gaagcatgtg ccgcatatgt cgtatatgcg gaatgcggca catatataga
    24541 aaggtatgag catggcagag cagacggtca ccgtaggtgt tggagcgctg agcatcgagg
    24601 acgttatcgc cgtagcgcgc ggcggtgcca aagtggcgat ttcggacgaa tcgaagcatg
    24661 agatggcgct gagtcgcgcg gttatcgatc atttggccga cgacaccgtg ccgcattacg
    24721 gtatctccac cggattcggc gcgctcgcct ccacctccat ccccaaggaa cagcgtgcgc
    24781 agctgcagaa aagtctgatt cgctcgcatg ccgccggtgc cggaccggaa gtggagcgtg
    24841 aggtggtgcg cggcctgctg gtgctgcgcc tgagcaccct gtgcaccgga cgcaccggtg
    24901 tgcgcccgga aaccgcgcag gtctacgccg atatgctgaa cgccggcatc accccggtgg
    24961 tctacgaata cggttcactc ggatgctccg gtgatttggc gccgctcgca gcctgtgctt
    25021 tggtggcgat gggtgaaggc gaggcccgca acgctgacgg cctcaagatc ggcggcggcg
    25081 aagcgctgcg tgcggccggc atcacgcccg tcgatttgaa agagaaggaa ggactggcgc
    25141 tggtcaacgg caccgacggc atgctcggca tgttgtgtat ggccatcacc gatctgcgcc
    25201 tgctgctcaa gaccgcggat gtcgccgccg cgatgagcgt ggaaggcatg ctcggcaacg
    25261 accgcgtatt cgccgccgac ttgcaggcgc tgcgcccgca tcgcggccaa ggcgattccg
    25321 ctgcgaacat cgcccgcatg ctcaaggact ctggtctgat tgaggccggc cgcccgggct
    25381 ccaccgtgcg tgtgcaggac gcctactcgc tgcgctgcac cccgcaggtg cacggcgccg
    25441 cccgcgacac cgtggaatac gcggcctccg tggcgggcgt ggaactcgcc tccgccatcg
    25501 ataacccgtg cgtcacgctg gacggccgtg tggagtccaa cggcaacttc catggcgcgc
    25561 cgctggccta tgtgctcgat ttcctggcga ttccgaccgc cgacgtagcc nnnnnnnnnn
    25621 gcctctatct ccgagcgtcg taccgaccgc ttccttgacg tggcgcgcaa ccgcggcctg
    25681 ccggcgttcc tggctggtga cccgggcgtg gactccggtt tcatgatcgc ccaatacacc
    25741 gctgccggca tcgtctccga aatgaagcgt aacgccgtgc ctgcctcggt cgattcgatt
    25801 ccaagctccg ccatgcagga agatcacgtg tcgatgggtt gggccgccgc ccgcaagctg
    25861 cgccggtcga ttccggcctt tgcgcgcgtg ctggccgtgg aactgctgtg ctcctgccgc
    25921 tccttcgact tccgcaagcc gtaccgtccg ggtgcggcgg gcgccgccgt ataccgcgtg
    25981 gtgcgccagt acgccaacga aatcggcccc gacgtacgcg atacctggac caccccgcag
    26041 attgagggcg tggccgaggc gattctctcc ggcgaagtcc tcgccgccgc cgaatccgcc
    26101 gtcggcgcac tgaagtagcg gctgcccaag cttcctcatc agagggggct actagtcacc
    26161 ctccccacca aaatccaaaa cttgagtcat atgcactcaa gttttgggaa tagaatgggt
    26221 tccaggtcgg ttgaccatat cagacaacga caagttgcgg tctgcggtgg cgcttgcggg
    26281 gctttcgaag cccgnnnnnn nnnncccgcg agccgctccg cgcgggccgc atgatatgga
    26341 ggaatacgat atggaacaga agttcaccac tatggcccag gaggccgtcg gcgacgcgat
    26401 tcagagtgcg tccgccgcag gaaacgcgca ggttgagacc ctgcacgtca tggatgcgtt
    26461 gctgcgtcag gaaaacggcg tggtgcgctc cctcatcgag gccgcgggcg gagacccaca
    26521 ggccatcggc gccgccgtgc gtaacgcttt ggtcgcgctg ccgagcgcat ccggctccag
    26581 cacttcgcag ccgcaagcca gccgccagct gaccgccgcc atcgcccagg ctgagaagga
    26641 aatgcagcag atgggcgacg aatacgtctc caccgagcac ctgcttatcg gcatcgcggc
    26701 cagcaagccg aaccagtcgg ccgaaatctt ggagaagaac ggcgtcaccg cagcctccct
    26761 gcgcaaggcc gtacccggcg tgcgtggtgg cgcgaaggtc accagcccgg acgcggaagg
    26821 ctcgtacaag gccctggaga agtactccac cgacctgacg gccgccgcca aggaaggcaa
    26881 gctcgatccg gtgatcggcc gtgatcagga aatccgccgc gtgattcaga tcctctcccg
    26941 tcgtaccaag aacaacccgg tgctgatcgg cgagcccggc gtcggcaaga ccgccgtcgt
    27001 ggaaggcctg gcccagcgta tcgtggcggg cgacgtgccc accaccctgc aaggcaagaa
    27061 gctcatctcc cttgacttgg gctccatggt ggccggttcg aagtaccgtg gcgagttcga
    27121 ggagcgcctg aagagcgtcc tgaacgaaat caagaatgcg gacggccaga tcatcacctt
    27181 catcgacgag attcacacca tcgtcggcgc gggtgcggcc gaaggctcga tggatgccgg
    27241 caatatgttg aagcctatgc tggcccgtgg cgagctgcgc ctgatcggcg cgaccacgct
    27301 ggacgagtac cgcgagaaca tcgaaaagga tccggcattg gaacgccgtt tccagcaggt
    27361 gttcgtgggt gagccgagcg tggaagacac catcgccatt ctgcgtggtc tgaagcagcg
    27421 ttacgaggcg caccacaagg tgacaatcgg cgatgacgcg ctggttgccg ccgcgaccct
    27481 ctcgaaccgg tacatctcag gccgtcagct gccggacaag gccatcgacc tgttggatga
    27541 ggcgggcgct cacctgcgta tggagctcga ctcctcgcct gaggagatcg atgaactgca
    27601 gcgcaaggtg acccgtcttg aaatggagga aatgcagctc aagaaggccg aggatcctgc
    27661 atccaaggag cgtctgggca agctgcaagc cgaactcgcg gacacccgtg agaagctcag
    27721 cggcctcaag gcgcgttggg atgcggaaca ggccggccac aacaaggtcg gcgacctgcg
    27781 tgccaagctc gatgacctgc gcgtgcaggc ggataagtat acccgtgaag gcaacttggc
    27841 ggaggcctcg aagattctgt acggagagat tccgtccatt cagaaggaac tcgccgccgc
    27901 ggaaagcgcg gatgccgaat ctgccgatgc cggcgcggcc aacccggccg atgagccgat
    27961 ggtcccggac cgtgtggacg ccgactcggt ggccgagatc gtctccgact ggaccggcat
    28021 tcccgtcggc cgcctgatgc agggcgaaaa cgagaagctg ctgcacatgg aggactacct
    28081 cggcaagcgt gtgatcggcc agaaggaagc catcgccgcc gtgtccgacg cggtgcgccg
    28141 ttcgcgtgcc ggcatctccg acccgaaccg tccgaccgga tcgttcctgt tcctcggccc
    28201 caccggtgtg ggcaagaccg aactggccaa ggcgctcgcc gacttcctgt tcgacgacga
    28261 gaaggccatg gtccgcatcg acatgtccga atacatggag aaggcttcgg tctcgcgcct
    28321 gattggcgcg gcgccgggct acgtgggcta cgagcagggc ggccagctga ccgaggccgt
    28381 gcgccgtcgc ccgtattccg tggtgctgtt cgacgaggtc gagaaggcca acccggagat
    28441 cttcgacgtg ctgctgcagg tacttgacga tggtcgcctg accgatggcc agggaaggac
    28501 cgtggacttc aagaacacga tcctgattat gacctctaac ctcggctcgc agttcctcgt
    28561 gaacgaggac atggacgccg acgccaagaa aagggccgtg atggacgctg tccacatgaa
    28621 cttcaagccg gagttcctga accgtctgga cgacatcgtc atgttccacc cgcttacgcg
    28681 cgaggagctt ggcggcatcg tggatatcca ggtggctggc gtgtcccagc gtctgaccga
    28741 tcgccgcatc acgctggacg tcaccgactc ggctcgcgaa tggctggcca acaccggtta
    28801 cgacccggct tatggcgctc gtccgctgcg ccgcctggta cagaccgaag tgggcgacca
    28861 gctggcccgt atgctgctgg ccggcaaggt ccacgacggt gacaccgtgc tcgtcgacca
    28921 gaccggcggc gaacatctgg agctctccgc ctgggccagc gaccagattg tctccgacaa
    28981 tccggacgtg agcgtggaca acgtcaccga agacaagtga caatcactgg ctcccctctc
    29041 tgagcaaagc tgtcagcgaa gctgactgag gggagttcgg gctggcgagc ttcactatat
    29101 tggctctcct ctgaggagga gagccttttc gctaccatgg tgctgaactt catacgagga
    29161 gggccagtga ctggacgatt cgcgccgacg ccgtcggggc gcatgcatat cggcaacgtg
    29221 tacgccatgt tgggtgcatg gctttcggcc cgctcgcgtg gcgaccgcat gctgatgcgt
    29281 atcgaggacg tggacaagcc gcgtgtggtc gccggtgcgg accagctcat gatggatgac
    29341 ctgcattggc tcggcctcga ctgggatggc gaaccgatgt tccaatccca gcgcactgaa
    29401 cgatacgaat acgctctcga atgcctgcgc tcgcaaggtg tgctgtaccc ctgtttctgc
    29461 tcgcgtgccg acattcgcgc cgcctcggcc cccaacgagg gcgacggctt catggtgtac
    29521 cccggcacgt gccgccgcct gctgcacgac catcccgacg aggtccgtgc ccgtttggtt
    29581 cgcggcgacc agcattccat ccgtatcgcc atgccggaat cggcagcggg agaaaagcaa
    29641 cgtacagtgc ctgacgattc cgctgcgctg tcgggagccg tgccgccgga acagcagggc
    29701 gatgccggaa tcgttgatgg tgtcgcatgc tttaacgacc gtgtctatag tccacagcac
    29761 tacgatttgg cccgcgaggt aggcgattcc gtgattcgcc gagccgatgg actgttcggc
    29821 tatcaactgg tcgtggtcgt ggatgacctc gatatgggtg ttgatgatat cgtgcgcggc
    29881 cgtgatctgc tgcgctccac cgcgctgcaa atgtggattc gccagtgtct gttggctggc
    29941 ggctttgagc cggaatgcgg caacacggaa aagcctctcg ccgagcaccc cgagtatgcg
    30001 cacctgccgc ttatcgacaa tgcggccggc cggcgtttag ccaagcgcga acgttcgctg
    30061 gatatgggag ccttgcgcgc ccgcaacgta acccccgagc aaatcatcgg atactgcgcc
    30121 tggctgctgc gtctgcaacc cactccaatt ccttgcaaac ccgccgatct gctggcagat
    30181 ttcagctggg agccactgcg cgccaaccac ctggaccgcg cgctgaaacc ggatgatcca
    30241 accactccac aatggctggc agaagcgtta ggctagaggc agcccaacag gaaggattcc
    30301 aatgtcgtcg aagctctgga gaaacgtggc gctggcaggc gtggccgccg ccggtgctgc
    30361 cgtgtgggcc gtggccccgc gcacgttcaa tgacaagcgt cgtaatttcg tgccctccat
    30421 tccggaggta tggtatgccc atcgtggctt gcatgatgcg ggttctggtt tggtcggcta
    30481 taccgatatc gctgattatg gtggggataa tccggttagc gcccaagccg ataccactga
    30541 gcgatccatc gccgaaaacg atgattatgt gtcgcttgcc cgtcgcatgg ctgtcaaggc
    30601 cggctacggc agtgcggata tgaccggtcc gattgctccc gaaaactcgc tggccgcgtt
    30661 cgccgccgcc tgcgaggctg gctatggcat cgaactcgac gtgcagctta cgctcgacgg
    30721 ccaagtggtc gtggtccatg acgccgattt gttgcgcgtg gccggcgatc cgcgccgcat
    30781 cgaagacctg acttacgacg aactcgcccg tattccgctc ttcccgaacg gcgagtcagg
    30841 cgatttgaag gccgaaccgt tgccgggtgc cgacgcgaac ccgccgctgg tggtcacgcc
    30901 gtccgccgcg ccgaaggggt actaccagca tgtgccgctg ttctccgatg tgctggacgt
    30961 ggtggccggc cgggctccgc tgattgtgga atacaagttc agcaacaaca gggcttggga
    31021 tgagcgtagc gaagagctca tggaaaaggg ccacgccttg cttgaggcgt atgacggccc
    31081 gtacgtgatc gaatccttcc acccgggcgc ggtgaattgg tacaaggagc atcaccctga
    31141 ggtgtgccgc ggtcagctga gctggccggc gaagctgagc aagaatggtg cggctgaatg
    31201 ggccgccggc ttactggcgt tcgactggct gtctcgtcct gatttcgtgg cttatgactg
    31261 gaccggtggc gcttcgccgc aagtcaagct cgctcgttcg atgggtgcga tgccggtttc
    31321 atggaccgtg cgcagcagcg atgaactcgc tcagtgcgcc ccctacttcg accgccacat
    31381 cttcgaagcg ttcgtccccg acgcagtgtg aggttgaggg cgttattgat gcgcccatgg
    31441 ggcggttaca ggctcatcgg gcggtaactt gcccatgggg cggtttgaag aatatgtgaa
    31501 accgcggaat taagccattt tggcataccg ccccatgagg attttgccgc ctcatggggc
    31561 aattaccgcc ccatggggga tttcccgccc catgaggtat ggtgttttac ttgtgatacg
    31621 gctcgccagc gttgatttta taagcgcgat agatctgctc cagcagaatc acgcgcataa
    31681 gctgatgcgg gaacgtcatc ttcgagaaac tcagcagaaa atcagcacga cgcagaatcg
    31741 cgtcgtcaag gccgattgaa ccgccgatga ccagctgaat atggctggtg ccacgcaatc
    31801 caaggtcgtt gattttggcg gccaattctt cactggacag ctgcttgcca ttgatggcaa
    31861 gggcaataac aaacgcgccg tctttcagat gcttggcgat gcgttcgcct tctctggcct
    31921 tgatctgtcg gtcaacgcct tcggaggcat ggtcaggcgt tttctcatct gccacttcaa
    31981 tgatgttgag cttgcagtag cgcgaaagtc gcttggaata ctcgtcaatg gcgtcgcgaa
    32041 ggtaacgctc cttcacgcgc cccggcgcaa taatgtcgat ttgcatagat gttccgttat
    32101 gttggctccc ctcggggagg ggagccaggg aaatcagtca attacgccat agagacggtc
    32161 accggcgtcg ccgagtcccg gcacgatgta gcacttgtcg ttaagacgtt cgtcgaccgc
    32221 gcacaccacg accttgaagt cgatggacgg gtcgatgtgc tcctcgacgt acttgatgcc
    32281 ttcgggggcc gcgatgatgt tgatcgcggt cacgtccttg gcgccgcgct cggccaggta
    32341 gtgcgtggcg gcgaccagcg ttccgccggt ggcgagcatc gggtcgatga ggaagcactg
    32401 gcggccggac aggtcctcgg gcagacggtt cgcgtacgtc acctgctggg tggggtgctc
    32461 ctcgtcgcgc ttcatgccca ggaagccgac ttcagcagtc ggaatcaggc nnnnnnnnnn
    32521 ggtcatgtcg tcgagcatgc ccaagccagc gcgcagcacc ggcacgatga tcgggcgcgg
    32581 ggaggcgagc ttcttgccgg tcatcggggc gaccggcgtc tcgatcggcg cggccaccac
    32641 ggacaggttg cgggtggcct cgtaggcctc aagcatcacg agttcggaga cgagctcgcg
    32701 gaaggtggag gagggggtgt tcttgtcgcg cagcacggtg agcttgtgct cgaccagcgg
    32761 atgattcaaa acatggagtt ccatgattcc cagcctagtc caagtttgcc gacctgccga
    32821 attggtaatg gcgcggcttg cttatcggat tggatttgtg ggtgattcga ctttttgctg
    32881 cgataggcca gttgcagcag gacaatgccc tgtccgtgcc gagatctgcc tctgatacct
    32941 ctgagagggt aaagcgtaaa aaacaacacg gtgtaaaaca gggaatgaaa ctggtgtaaa
    33001 accgggaatg agataggtgt aaaatcggga atgaaactgg tgtaaaatct atattgttag
    33061 aacgatagaa ctgggaaagg ctgcaatatg aacgatttga gtggtcgcga tggtaccttg
    33121 accaggcctg attatcgttc acgcgtggta gatagcagat taagtaaact gctcaaggcg
    33181 ttcggtgcag tggaaatcgt tgggccgaaa tggtgcggca agacatggac cgcgctctcc
    33241 cggtctcgaa gcgttgatcg tctggatgat gtggcagttt tcgcggccgc ccaaaccgat
    33301 cctgtattgg tgctgatggg ggaggagccc catctggtgg acgaatggca agatgtaccg
    33361 caaatttggg atgccgctcg tcgccatata gatgacaatg cgaaccgcaa ggggcagctg
    33421 attcttacag gttcggctat tccaaaggat cgtgacgcca ttcatcacag cggcactggt
    33481 cgcattgctc gtctgcgcat gtggcccatg agcttggctg aatccggaga atccacgggc
    33541 gcggtcagcc tatccgattt gttcgagggg aaattcgagc ctgccgaatg caatacgagt
    33601 atcgaagaca tcgccagatg gtgttgccgt ggcggatggc ccgcgaatct tgagctagac
    33661 gatgaattcg ccttggaaat cccgggcgaa tatatcgcaa gtgtgctgga tatcagcgtg
    33721 ccgaagatga agaagaatcc tgaaactgca cgagctttga tgcgagctct tgccgtgaac
    33781 atctcgcagg caccaaaata tgcgacgctg gctgcggata tggcatatgg cgatgagcgg
    33841 cgtgagccgg atacgcagac ggtgaagagc tatttggaaa tgttcaagga cttgtacctt
    33901 ctgacggatt tggagggttg ggaaccgcca ctccgcgcaa agagtcgtgt gcgaaccaag
    33961 cccaagcgat atttcgtgga tccttcgttg cctgctgcgg ttttaggtgc cgcgccgaat
    34021 actctgatgc gcgatacaca gacgctcggc gatctgtttg aaacgctgtg cattcgtgat
    34081 ttgaatgtat atctgtcggc catgccgggc gcgaacaatc gcatcgccta ctaccgcgat
    34141 gacaaggggc ttgaagttga tgtcattatc gagttgtctg acggcaggtg gggtgccatc
    34201 gaaataaaac tcagtgacct taaggtgaac gatgacaatg cggataagct caaatccttc
    34261 cgcaacaaaa tctgcggcaa tcccatggct caggtccgag accccgaatt catgatgttc
    34321 cttaccggcc gaggtgggaa ggcctatcgc cgcaatgacg gcatactggt cgtgcccatc
    34381 gcaacgctcg gggcgtgaat ctggtaaaag aaaagaagcg ccgaaccgcg aaagcgcctg
    34441 tcggcgcaag ggccgctcgc ggcggcaaaa aatggagccc ggggcttaag cacggtccga
    34501 cgcggatact tagtgtagct tgcgatgggt ctcgccgcgc cggggaaggg gtggcgttgg
    34561 ccggtagctg attgttgggg tgcggtcggt gggtttctcc ctcagtcatg ggcatcgccc
    34621 gtgacagctc cctcatcaga gggagcctgg agcggggtta ctccaaccgt ccgcgacgcc
    34681 agaggttggc accgtgttgg aaatccttgg cggtggccat gaggctgccg gcggtgtgca
    34741 taaggcgggc ggctttggtg cgcacggcct tctcatcggc atccgaggag aagccgccgt
    34801 tggcctcggc ccgggtcacc atgcgccgcg cctccacgcg caagcccaac gccaccacat
    34861 cggtaaacgc cgccgcacgc tcaagcgcca cggcaaaatc gcccacaaaa gcaccggaga
    34921 gaatcgaatc ggccaatcgc acgatgtcat cttcactggg agcctgatcc acgccggcga
    34981 tggcggaggc ggtagtggcc accggctcac ccaagcgcca cagatgcgcg atcgagtcgc
    35041 gattcgatcg catccacgta cgcaacatat acagtcgcca cagcacacca ggcagcgaat
    35101 ccggctcggc atcgctccat agttcggcga tttcatccac gccgaccgtc tccaccaacg
    35161 tgagcacccg ctcaacaacc gccgggtctt cgctatggcg cacacgatcc agcaatgcgg
    35221 cagcagacag atgactcatc tcgctcaacg cctgcgggtc catgccaccg gccaatccat
    35281 cggccacagc cgggtcgagc tgagccgggc gcgaggggcg tgaagaaccc atgcgagcag
    35341 gtccgaatgc cgctccggcc gcacgcaacg agccgaagcc cgggcccaga ctgccggcgg
    35401 caccattgtg cgcggcatga gctgcagcgg cagcggccga ggagttggat gaatcaggga
    35461 atgcgggtct agtagacaag cggttgaacc ttctgaatat cgatgatttt gggaccatga
    35521 ggcgtcggag tgacgaacag cgccaccgcc gtgccggtcg gcatgaacgg tgtcttggcg
    35581 atcaggcgtt tgctcagaga cggcgcaacg cacatgctgc gcaggtgtcc gaacatacca
    35641 ccaatcaccg gccggtgcat gcatatcgct atgggctgac ggcgctcaag tgcgaactcg
    35701 atttcgctga gtagacactc ccatgcggtg tccggcgcag cggcgtatgc atcttcggtg
    35761 agcggatcga gatgaaccat gtctcgtccg gtctgccacg cgaacatctc caacgtctcc
    35821 tggcagcgca cccaaggcga cgtggccagt cggttcggat tgaaacacgc cagctcgcgg
    35881 ttcagcgcgt atgccgcagc cgcgccgcgc ggcgtgatcg gacggttctc gtccgagccc
    35941 ttccacagtt tgcgcgcttc tgccttgccg tggcgcacga taatcaccgg cacggcgtca
    36001 agcgcgccct cctgcacccg gtcaacgaat agcgcaagga tgtccttatc ggtggaatgg
    36061 ctgagtttct tgcgggcctc agccggcgtc aaccacagga cttcgtcgat ttcgccgatg
    36121 tccgcccgat gcaccgggcc aaacgcatgg gtgcgccgca gattgtcgat ggcgctgatt
    36181 ggcgtggcca tccagaactg gatgtgcttg gtgtccgccg accgatcctt ggtatggcgc
    36241 tgtttgcggc cctcttcgga cagcgggtac tcgatgtcgc ccagataggg accgagctcc
    36301 acgggcaggc ccgattcctc gccgatctcg cgcacggcgg catgccggtg tgattcgttt
    36361 gggtcgacct tgcctttcgg ccagctccag tcgtcgtatt tcgggcgatg cacgatgcac
    36421 agttcgatgc ggttgagtac gttgtctgct gtggctgggc tgtcggcctg cgccgggtcc
    36481 ggggtggaag ccgcgtccga tgcgccgggt gctgccgtgg tctcgccgtc agaatccgtg
    36541 gtgttcgcga tgatggtgcc atcggtggca atattggatt tcactgatgg agatgatgcc
    36601 cgcgtcggct cggcatcatc cttccaccgg tacagaatac cgccggcggc ttccacgatt
    36661 cgtctcatac tgttcgtacc catctcttgt attgtaggtc acgttggacc gctgctacgt
    36721 agtttttgcg cgctgaacaa cgatgcccgg catcctctcg aaagaggagc cgggcatcgt
    36781 aaccaccctc agttagcttg cgctgacagg gagcatcgag tgaaatcagt tgtgtgaatt
    36841 ggggcgacgc tggtgcttgc gaatcaggta ttcctgacta tcgaccagcg ggcggccctc
    36901 gtcgtccttg gtgtgcagca catacgtgcc gtcgggctgc atgtgccagc tcatggtgga
    36961 gtcggccatc tgcaggtcca cgtacttgat gagctcgtca atctgctccg gggcggtgac
    37021 gcgcaccaga gcctcgacgc ggcgatcaag gttacggtgc atgaggtcag ccgaaccgat
    37081 gtagacctcc gggccggaag ccgggccctc gccgatctgc gggccgtccg cattgcagaa
    37141 ggcgtagatg cgggagtgct ccaggaagcg gccgagaatc gaacgtacgc ggatgttctc
    37201 ggacaggccg ggaacgccgg gcttcagagc gcagatgccg cgttcgacga tgtcgatctt
    37261 cacgccggcc tgagaggcac ggtacagggc gtcgatggtc ttctcgtcca cgatggagtt
    37321 gaccttgatc ttgatccagg cctccttgcc ggcgcgggcg gcgtcctcct cgcggcggat
    37381 gcgctgaatc aggccggtgc gcacggtacg cggggccacc agcagacggt ggaagctgga
    37441 cttcggcgcg taaccagaga gctggttgaa cagacgagtc agatcctggc cgaccaccgg
    37501 gtcgcaggtc agcagaccca gatcggtgta cagtcgcgcg gtcttcgggt tgtagttgcc
    37561 ggtgcctacg tggcagtagc ggcgcagacc gtccgcttcc tgacgcacga cctcgatgag
    37621 cttgcagtgg gtcttcaagc ccacgatgcc gtacaccacg tgcacgccgg cgcgttcgag
    37681 cttgcgggcc cacgcgatgt tggcgtcctc gtcgaaacga gccttgattt ccaccaaagc
    37741 cagcacctgc ttgccggcat gagcggcgtc gatcagggcg tcgatgatcg gcgagttgga
    37801 gctggtgcgg tacagggtct gcttgatggc cagcaccttc gggtccgcgg cggcctgcgc
    37861 aaggaaggcc tgcacggaag tggagaagga gtcatagggg tgatgcagca ggatgtcgcg
    37921 ctcgcggatg gcagcgaaaa tatcctgtgc gcgggaggac tcgacctcgg cgatctgacg
    37981 gttcgtggtc ggcacgaacg gacggttctt caggtccggg cggtccacgc tgccgagctc
    38041 aaagagcacg gtcaggtcca gcgggctggg cagacggtat acctcgtcct ggctcacgcc
    38101 gagctggtcg gcgagcagct gggagaggaa ggggctcgtg gtgtccgtga tctccaggcg
    38161 gatcggcggt ccgaagcggc ggcgcagcag ctccttctcc atggcgttga gcaggttctc
    38221 ggcgtcatcc t
//
LOCUS       DAWBMW010000003        35671 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_373_length_35671_cov_3.488966, whole genome shotgun sequence.
ACCESSION   DAWBMW010000003 DAWBMW010000000
VERSION     DAWBMW010000003.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 35671)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 35671)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..35671
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_373_length_35671_cov_3.488966"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    906..945
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    12146..12155
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    23781..23790
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    24121..24130
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         7012 a        10829 c        10620 g         7140 t
ORIGIN      
        1 ggagatggcc ttgtcgatgt cgaagcgggc cgggttggcc ttgacgtcgc gcacgtcgaa
       61 cttctcggtc atctcgtcca tggagaacac gtcgcggtcc ggtgcaatgg accagccgag
      121 cagggccagg tagttgagca ggccctcgcg gatgaagcca ttgtcgcggt ggttgaacag
      181 gttggactct gggtcgcgct tggagagctt cttgttgccc tggcccatca cgtacggcat
      241 gtggccgaac agcggcatct ccttggcgat gccgagttcc ataaggtaac ggtaaagcac
      301 gatctggcgc ggggtggagc tcaggaggtc ctcgccgcgc agcacgacgt tgacttccat
      361 catcgcgtcg tcgaccgggt tggtcagcgt gtacagtggg tcgccgttcg ggcgcacgat
      421 cacgtagtcc ggcacggagc cggccttgaa ctcgatggtg ccacgaatca gatcgtcaaa
      481 ggcgatgtct tcatcaggca tcttgatacg tagcgcaggc ttgcggccct cggcgcggaa
      541 ggcggccttc tgttcatcgg tcaggttgcg gtcgtagcca tcgtagccga actcggccgg
      601 acggccggcg gcaaggttgc gctccttgat ttcctcagga gtggagaagg actcgtaggc
      661 gtagccggct tcgagcagct tggcggcaac atccttgtag atggcggtac gctcggactg
      721 gcggtacggg ccgtgagggc cacccacatc gatgccctcg tcccagtcga tacccagcca
      781 acgcagggat tcgaggatct ggttgtagct ttcttcggag tcgcgcacgg cgtcggtgtc
      841 ttcgatacgg aagatcagcg tgccgccggt ggcgcgagct tccgcccagt tgaacagggc
      901 agtacnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngacat gcggggttcc
      961 ggtcggcgac gggcagaagc gaacacgaac gttcttgggc agttcgggtt tggtgttttc
     1021 agcatcagtc atagtcccct attgtgccgc gcgcgccgga cgcggagagc agcgctcctc
     1081 gctcttttgc ttcgttattg acgtttcggg ccaacaccat cccaaaacgg ccattttcca
     1141 acgaaatctc ttttgcctgc cattttcttt tcttttgctt ttaaaatcaa aagaaaagaa
     1201 aacagcaaac gagaatcagc aggccaggcc cacgaacagc agatcctagt cttgagattc
     1261 gtcttcatgc gattcgcgct gctcaacggc ggcatccgtg tattcgtggt tcatggcatt
     1321 ccctacatct ctgtatgttg ctttatgcgt agcgccaaaa gcttcgtctg ctttccagtt
     1381 ccacccccgc aactgtgcgc aatattcatg taacgggggt gcggatgttt ttttggacgg
     1441 tcaggcggcc agtccaagac ttctgcggta ttgcacgggg ctcatccagt ccagtgactt
     1501 cttgatcctg gtttcattgt agtgggtcag gtaggcggcc aggcgttcgc ggaactcctc
     1561 gtagcccacg cccctccagt cccgcccgta gaataactcg ttcttgagcc tgccgaagaa
     1621 cccctcggcg gccgcgttgt ccgggctgca tcccttggcg ctcatcgacc ggatcagccc
     1681 gtgctcctcg cagatgcgga tccattcgtc ccaccggtag tggcagccgc ggtcggaatg
     1741 gatgattgga tgttccccgt ccctgagcgt ggcgaccgcg tcaaggagca tccggttcgc
     1801 catgtcggcg ttcggggagc gcgacaaggt ccaggagacc accatcccgt cgaagcagtc
     1861 gaccaccggg gaaagccagc acttgtaccc gtccatggtg aactgggtga cgtccgtgac
     1921 ccacagcatg ttcggcgcgt cggcgtggaa gtcccgctcc acgaggttcg ccggggcctc
     1981 gccgatctcg ccggcgtagg agctccaccg cttcggccgc ttcaggtaca cgggccgcat
     2041 ggccccctcg cgcatgatcc gcctgacgac cttctccgag acgacgatcg ggtcggactc
     2101 gtcaaggcgc agcatgcggt ggatggtccg gtatccccat gcgcgcccac cctgctcgaa
     2161 caggccggcg acgcgggccc gcagcacggc gtacctgtcg cccgcggcga tcgcgccgcg
     2221 ctcgtaatag taggtgctgc gtttcagccc cacggccttc agacaggccg ccaggccgaa
     2281 ttcacccctg atcgcgtcga cgacccgcgt cctctccctg ttcgtcagca tggacgggtc
     2341 gaggcgtggg tcgtcggctt ttaaaacatc gatcgtctcc cgcatcaccg cgttctccag
     2401 ccgcaggacc tccacctgcc ggcgcagctc ctccacgtca tcgccggccg cgggcatcgg
     2461 ttcggccggc atcggcgcgt ttctcctgtc catcagtccc agtatcccct cgctccggta
     2521 gcggcgcatc cactggtaca cgctcgtcgg cgtgcaaccc gcgtcgcgcg ccacgcgggc
     2581 gaggggctcg ccctcgagcg cgcgtctggc cgcattcgtc ttgcattcca gcgtatacga
     2641 acgcctgcac gcgcccgtgt aacgcgggtc ctcgcgaacc catttcacca gcgcgccctc
     2701 ggacgggtaa ccaagctcgg caatgacctt cctgatggtc atgccctccg tgaaatacag
     2761 gtcaacggcc ctgcgccgat cctccatcga aaacatgcgg actccaatcc ggacgcccaa
     2821 gcgtccaact ttccgtccgc atccccaaca ggttacacaa ccgatcgata acgttcaccc
     2881 tcaactgata tcgatttgat aaccatcgat tctttccagt gcattcacca caactttgga
     2941 cattatttgg cgcaattgtt gagttttcca ctcatgcaat ggaagaatat gatgtgacac
     3001 ataggtctta tgaaagggga atcaatgtct gatcccaaca atgtgccttt gcctcagcag
     3061 cctgaggcca acggtacgcc cacacctaat cccgtaccgc agtatggcgc accggccccg
     3121 gcgcagccct cacagccggc gcagcccgct tacggacagc cggcatacgg tcagcctgct
     3181 ccgaccgcga actcttatgg ccaggccgta ccaacctacg gtcagcccgc gcccgacgcc
     3241 aacacctacg gtcaatccgc accggcgtac ggcacaccgg cccccgatgg caatccttac
     3301 ggcccggttc cgccgtctta cggccaatac gccgcgccca ctgccactgt gccgctggat
     3361 aagccttact acggatgctc cttccaagaa gccttcctgc gcttctggaa gaagtacacg
     3421 gtgttcaagg gccgcgcgtc ccgcagtgaa ttctggtggt gggtgctggc cgctttcggc
     3481 atcaatatag tgctggacat cctcaacacc gccacagatg aaaagcttgg cttcctcgcc
     3541 accatttggg gtctggtgac cctaatcccc accctggcac tttccgtccg ccgtctgcat
     3601 gacacgaaca agcctggctg gtgggttgcc attttctata tcgcgatggt tatcggcctt
     3661 atcatcatga ttatcggcgg tggtgcggca ctgtacggcg ggttcaggag ccttggcagt
     3721 tatgactatg gttatggcag catggccgcc ggcggtcttg gcgcggcggt cattggtgcg
     3781 ttgattacgc tggccggctg cgttgtctat atcgtgttca tggctcttcc cagcaagccg
     3841 gagggcgcac gttttgacga tggcgcggcg gcaaccccca tgcctcaggg tgcttacggc
     3901 gcaccataca gcacgcctgt acccccgaat ttcgccgctc cgaacgcgcc cgcacccggc
     3961 ttcaaccagc cggccctggc ttacgtccag ccggccatgc cgacccccga ctatggccag
     4021 ccggctgcac ccgcacctga ttacggtcag gtaccggccc cgcagtacgg ccaaccggag
     4081 gccccggccc cacagtacgg ccaggttccg ccgatgcctc aaaacgccgg cgacgaatcc
     4141 gcaacacctt ctgagcacac ggctcccgcc ggcgacaatg cccagaaacc ctggcaaggc
     4201 cagtaaccga tatcggtgaa cttccgttga agaagttccc cgcaaccaat gatgaagccc
     4261 tactcaacgc gagtagggct tcatcattct cggcactgta ttatcggcac tgtattcagc
     4321 caactacccc aacggcatca cggcaatgga ctgcgaaagc atatacgatt cacccggact
     4381 aagggcaacg atacggtctc ggcacgccgc tgcctccacg gccacaaagc cacgccattc
     4441 acccgtcttg gcatgggcaa tggcgttgcc gccctgctct ccgggattcc acaccaccgt
     4501 ctgcggcgag ccggtcttgc cgatgcgaat cacacgaccc agcacatggt cctgcaattc
     4561 cagtgcagca tcggaataat acacacggtc caccggctga tcgccgaaca tcaccgatgg
     4621 ctgttcttgt agttttggcg ggaatccggc ttccgtggca tccagatatg ttgcaccccg
     4681 caaaccgacc agccgcgcgc cgagcacatc gccgacatgc agatacgagt gcagcgccgc
     4741 ctcatacgac atcggctcac ctcccgtgtt ggtaacggtc agcgtcatcg tcagcgtatc
     4801 gctggcacac acctcgtatg cggcatggaa acgcacgttg ggttcatcct cgatccatgg
     4861 cacttcgcca gtcgtcagtc gttcggaatc cagcacatac cgcacacggg caccggtaaa
     4921 cgccccttcg tccagcgccc actggcgcaa tcgtgcgaac ccatgaatcg gtcgcttcgc
     4981 agtctgcccg ccatgcacaa accccggccc gaaccagggg aagcacaccg gcactccccc
     5041 gccaaccggc tgaccagctt cgaaatgcca tgtgcggggc gtccagatta catccggctg
     5101 ccccgccggg gcccagcgca gcagatgcgc cccgtaatcg ctgattaccg cgctaccgcc
     5161 gtcattggtg agcgtgcgca gattgacgcc attgcccata agtgtttctt cttccgttgc
     5221 gtaagcgatg ttcttcacca catagacgat ttccttggca cggaggaatc gcgagcaggg
     5281 gtaagagaac cgatcaggcg ggctaagctg ccttgattct cacaactcca cggtttcttt
     5341 gccagggtca tctttaatgt gcgcaaactt ggcggccaag gcctccttgt tgcgcttttc
     5401 gtcttcttcc ttggccttct tcttggcttc gtccgggttc cagagcacat cgtcgcggtg
     5461 cttggtccat tcggcaccgg ccagcggggc aatgtattcg gcggtggcgg tcagggtgtc
     5521 accggcattg gcacgcgtat caatcagatc aagaatctgc tggtcggtca ggcagatgcg
     5581 cggaatcgag cactgctcac gtaattcgtt gacgtacagc agaatcggca acgttacgat
     5641 ggcggtggcc cgggcctcgt cagcaacatc agcggcatca gcaacagccg gaccaaccaa
     5701 gccaaccgtc tcggcatgcc cgctcaccac gttcagcagc gcatcgcata cggcgagcgc
     5761 ggtggcgaaa cggacggcca catcagccgg ctcgccggaa gccgccaaac gtccgccagc
     5821 ctcggcatcg gcctcaacct gagcctccac ggagacaacc gtctcacgga ctgcggcctc
     5881 gacatgcgaa tcccagtcaa cacctaaagc ttcggcggca tcgaaaatca gctgcacatc
     5941 gccggattcc gagtaaccgg ccatcgtatc gtaggcgtca ttggcagcaa gcagcgtgtc
     6001 tacgatgctt tgcggacggc caggaatctc atcggccccc tcgtagacga attcggcgtc
     6061 aggcatcgta acatcctgac cggtgagaat gcgtgcgaac gcgtgcgtga cacgcacctg
     6121 caggttctcc gcatacttgg cgtcattgtc catctgcatg gcttcggtca gcggccacag
     6181 gctaattagc gccgagccgg cttcagcggc ctcagctacc cccggcaatg ccgcgatccg
     6241 tgcgattgcc tcacgattga gttcaatggt cattcatgct cctctgcgtt caccacttga
     6301 ttgtagtgcg tcccattggc tccctctgag gggagcccag attgtagctc agctattcac
     6361 cagtgtgccg ttacttgacg ttggctgctg ggtcgacata cgtgattgac atcacacggc
     6421 cggtttcgtt gtcgtacgtc accgaggtca cggaggcgag cgccgtgtgc cgcaggaaca
     6481 tccagtgctc ggggtggccg gtctccagat aatggcgata actgaaaatc ggcgattcgt
     6541 ggctgaccac cacaatctgt tcgccggggt gttgggcgac cttctcgcgt gcgaaatcat
     6601 ccatgcgctg cgcaatgtgc tgataggatt cgccccaact cggcttgtac aggttggtga
     6661 ccagtttcca attgccgttc ttccacagcg cgccttcgcc atggccgata cgcttgccgc
     6721 ggaactcgtt gccggcctca atcactcgct catcggcggt gagcatgagc ggggcttcgc
     6781 ctcgggcctc gcgcacggga ttcaacgcgt caagaatcgc gtcggcggtc tcgcgcgtgc
     6841 ggtccagggg agacgaatac accgcggaaa tcgtattggt ctgagggcta ttggccagat
     6901 aggcggcggt ggcctgtgcc atacgccggc caagatcgga gagatggaag tcgggcagcc
     6961 gctcatagag cagatggtca gggttgtaga ccttgccgtg gcgaacgaag tggatggtgg
     7021 ttgcgctcat gagccgcctt tctttttatt cctacgggca tcttgcgatg ttcgccgaag
     7081 aacatgagaa cgttgtctta atatctccaa tctaccgcgt gtcgcgccct aaccaacgat
     7141 tagagtatcc gtgacgcaaa tatgcggatg ggggaaaatc cgacgcaaac tttgcgcgac
     7201 catggacaac acatcattcg atgagggctt cgccggcaac gccacgtcag cggggaacat
     7261 ccaatcggga gcggaaagga gcgtagtgct gttatgtcca acaacctgtc caactcgaac
     7321 accattatgg ctggcatcaa ggaagtgggt cacaaagttt ttggtgggta tgcggagttg
     7381 ctgcgcatac ctcataccgc tcgttattcc atcggttcgg tgattgcctg catgccgttc
     7441 ccgatggtgg gcatgaccat caccatttcc gtgcagcatt actacggcaa ctactcgctg
     7501 gcaggtatgc tcaccgccat tcaggcgatt gcactggccg tggccacgcc gctgctcggc
     7561 aagctgaccg acaagttcgg ccagcggcag gtttcgattc caacgattct ggtgtggatt
     7621 gtcgccgccg tcgcgctgac cacatccatc accaatcgtg cgccggaatg ggtgctgtac
     7681 tgtttgacgc cgttcctggc agctattccg ccgtggggcg cgatgagccg cgcgcgctgg
     7741 accaaaatcc tgaagggcga tcaggagcgt accaaccgtg cgctctccct gtgcggcgtg
     7801 ctggacgaat gcatgtgggt aatcggcaat ccactggctt ccacactggc cgtgatctcc
     7861 ggtctgctgg cattctcgtt caccggcatg tgcgtggtgg tgggcgcgct gatgttcttg
     7921 accgagctga ccactgagcc accgtcacag actcagctgg cccgcgctga gggcatttcc
     7981 cgcaaggaat accgtgagcg cgaggcggct cgagccgaag cgttgaaggt ggagaccgca
     8041 gccgaggaga ctcgccgccg tcttgagcgc gaaggcgtga ccgatgaggc cacgattaac
     8101 gccgccattg agcaggcggt cgccgatgcg cgttccggca agaaggcgtc catctgggga
     8161 ccgggtctga ttgcggtatg cgtcacctgg ttcggcttgg gcgcgttcca gtcggctaca
     8221 ggcatctcga tcatcgcatt cgccaccgag tcgaatatga agcagtacac gggctttgta
     8281 ttcgcctgct tctccatcag ctcgttgatg ggcgccttgg tgtacggcgc gaagaactgg
     8341 tcgattgccc tgtggaagcg cttctacttc tgcctggccg tggtgaacat cggcatcagc
     8401 acgttcctgt tcgccaagca tctgtgggtg attatgatca tctacctgat catcggcgtg
     8461 tgccaggctc cgacgtggat taacggcaac cagctcatgc tgcacttggt gccgccttcc
     8521 cgcctgaccg aaggcatggc gtggatgggt gcgatgaatt ctatcggctc ctccgccggt
     8581 tcggcgattg ccggccagtt catcgaccgc atgggctcgc atggcggctt catggtggtg
     8641 accactttgg cactggtctc gctggtgatt gcgctgattg gcttcaagca gatcaagggc
     8701 tccaccgagc agccgacctt gactgaagtg agtgtgtgag acaaaagcta agccccctct
     8761 gacgaggggg cttatacgtc aaaacgctac ttcggccagc ctttgggcgt gtgttccagg
     8821 gaccagatgc cgagcttgtg ggagaagctg ttctgccact tggcttcaag ctcctcggca
     8881 ttcagtcgat tttcagcgga cttcagacgg ttcatcgcat tggtgtgcgt atcgtcgagc
     8941 agccacttgc gaatcaagaa gttccagatc attaccacgg cagtggccac caacttgccg
     9001 atattggtgc gcaacagata ttcggcatgc tgcgatacat aggcatcatg gttcatgcca
     9061 taagtggaaa tccagataat cgcgtcattc atgaacagac cgactacagc gcccgccacg
     9121 aagataagaa tctccatcca gcgcgccatg tcatcacgat gcttgaatac gaatttcatg
     9181 ctcgccagat agttgaaaat cagcgagatg atgaaagaga cggtggcagc cagcacgttg
     9241 tgcatgtgga atatgccaac caataggttg agaatacccc agtcgataac aaaggcgatg
     9301 acgcccacaa tgccgaactt catcagttgt tcaatcagtt ttctcacaag ggtattcaac
     9361 actattgggc tgactgcgga acatatattg gctggaaagg ccaataatct ccccaaattg
     9421 gccttgcccc ggtccgttct gcaagcagct tcctacatcc gcatgccatg ttcaacagca
     9481 accgctgagg ctagactgga agcgaactga atcaaggagg aattatgccc gttattcaca
     9541 ctcatgtgtc cgtgcccacc acccctgagc agcgcgaggc gttgaaggct gcctatggca
     9601 aggcgattac cgccgtgccg ggcaagtccg agggctggct aatgtgcccg ttcgaggaca
     9661 atatgccgat ttacttcggt ggtagcgatg accagcccgc cgcctatgtg gaagtcaacg
     9721 tgttcggccg ctctgtgccg ggctctgctt gggaaaagct gaccgagagc attatggccg
     9781 cgctgaactc cacgctcggc attcccgagg accgcaccta catccgttac accgccacca
     9841 ccgactgggg ctggaatggt ggcaacttct agttgccacc attcccctcg cagcatagct
     9901 gctcgcatcg cctacggaaa gcccggtggg ctttccgttt aacggctcag ggtagcaaat
     9961 tctggctccc ctcagtcagc ttcgctggca gctcccctca aagagggaag ctcagatgta
    10021 gaaagcccgc cggcggcggg ctttctacgt ttcaaggggc tgatttccaa cattcagaag
    10081 ctctactagc gtttcaaggg gcttttaaaa caagaaaccc agagtataaa cattgcaatt
    10141 tcaatggtca tactccgggt ttcgcaatcg aagagcccct cgaaacgcag atagacctcc
    10201 ggaaaaccgg aaatccgccc ctcgaaagcg agttacctca ccttgtgcct gtcatcacac
    10261 gatcttgggc atgggggtgg tgaggctggt ggtcaggttg acgtggacca gaccggcacc
    10321 ggcatgcacc tcgaccttct tcagggtcac ggtgcgctct tcttcggggg cacggtcgtt
    10381 gtcggtcatg gtcgccgggg acttgaccgc cacgaacgcc agatcgtcca gcgagatgcc
    10441 tgcgctctgg cacagttcca tggcctcgga cacggactcg gtgaagcccg gcttgtaaat
    10501 cacgcgctcg gccggtacgc cgtaggcgtt ctcggccacc cacttggcgt tctcggtcag
    10561 ggccatgccc ttgtgcgagt gcggttcggc cggcatggag ccgttctgca acgcgtcgac
    10621 gaaaccgtca agctgcgggg ccagcgcctc gccgccgtcg cggaacaggt tgccgatgat
    10681 cggcgtgcgg aagcccagct cgtcggcggt ggccttcagg gacggaacct gatcgtcctc
    10741 gccttcccac aggttgatga gcgggaaggt cggaatgtcc aggttctcca ggcgctgcac
    10801 gtggaacggg tagcgccagg ccagctcggg atcgtcgcgc caggtggagg cacgggtcac
    10861 cacgatggcg gcggacggcc actgcgcgcc gtattcgcgg caggcgatgt ccagccactt
    10921 ctgggcaccg gcgtcggtgc cgtagccggc ctcgacgatg accacgtcat gcagggcgca
    10981 ggccatttcc acggagacca acgtcggaat gccgatggac acgttggcga acgggccacc
    11041 gtgcacgtag accggtgatc cgttcacggt ttcggtcttg gccggcttga ccgcgtcggc
    11101 caaaatgcca gtgatacgcc acaggtccac gaattcgccg aaggtcacgg ccttgccgtc
    11161 cttggtgccg gcgatcatct tggacacgcg ctcggaaatc tcgtccatcg agcgggagag
    11221 caccacaatc tgcatgagct cgcaggtcgg cgtgagcacg accttctccg gcacgttgcc
    11281 cttgccgtag tccacagcgg tggagcgcag ggagcggctc ggaacctcgg agacacgcgg
    11341 cacgagaatc gtgtcgagct tgccgtcatc gatggccttc tcggcgaagg agacgagcag
    11401 gttctgggcc gcctcgatgg cgcccatttc gccgcacaga ccccagtcga tgagctcggg
    11461 gtgggtcagg gaagccttgc cgccgccgga ggcaccgccc ttggaaccgg ctgcggtgat
    11521 gcccatgctc ggctgacgca gcacggcggt cgcatcaatg ccgcgcttgt tcagagcgtc
    11581 gatcagggcg atggtcgtgg tggtcttgcc ttctccgcgc gatgccttga gcggcgtatc
    11641 ggcggtgacg agcaccacct tgccgtgctt gcgcggggcg tcagggttct cggccaggta
    11701 cttcatgtag ccgaatgcgt cgatcttctg gaccagaccg tactggctgg tgaagtcttc
    11761 gatggtggtc atataaagcc tttctgtgtt tgtgtggtgg ggcggggtgg tggggcggca
    11821 gtcgccgcct cttcgcctaa aaacagccca ctgggctgtt ttcttaacgg ctcagcccct
    11881 cagggagggg agctcaatac ggactaaaag tcggacatgt ggaagccgtt cttcatttcc
    11941 atgctgcgag tgtacagcac ggagtcaagc agcttttcgg tttcctcctt gagctggtcg
    12001 gccatggtct ggttggcggc ggcgagaatc tcgcgcacct cggggttacg cagcgcggcg
    12061 atggtctcgt ccggggtcat cggctgcaca tcgccatcca cgttgtcggc ctcaaattcc
    12121 tcttcagcgg cggactgcgc ggcttnnnnn nnnnnctcaa cggcgttgga cgtggagcgg
    12181 aaggcgccat cgcacagggc ggcgatgatg cggttggccc agtagaagtt ctcggacgtc
    12241 acgcgggtgg tggtgtcctc aaggtaggcg ggcgtggtgt ccacgttcgg gaagaacggt
    12301 accagcgtgt tgaacgggtt ggagccgtat gccatccact ggatggcgcg ggaggcctgc
    12361 gggcggtacg ggcggatctg catcaccgcg agctggctct ggcggttgat gccgatcgtg
    12421 cggtacatgt ggcgggtgcg ctcatcgccc agctggccgt acggatcgaa cggggtgccc
    12481 tggtagtggg agctcagcac gtacttgata tcctcgatgg tcaccttgcg ctcaggctga
    12541 cgggcccacg ggatgtcgtc ggaggtgggc ttgtggtcgg cgtccgggcc gtcccacacc
    12601 tcgtcgtacg ggttgaggaa gcgctgcatg taccaggcgc gcggggtgtt gtacacgtgg
    12661 tcggagtcgg agtgggagcc gaaggcatcg cgcgggttga acggggtagt gttctccacc
    12721 gcgaggtcca aatggttggt ttcgatgaat tcggcaaggt cttcggaaca catgtgcgct
    12781 tcctggtcac ccagagcgtc ctcaaggtcg aattcgtcga taccgagctg gttcggcatg
    12841 gtcacgtagg cctcgtccgg cacgcgcttg gcaatccagt gatggccgcc cacagtttcc
    12901 agccaccaga tttcgttgga gtcggagaag gcgacgccat tcatctcgta ggtgccgaat
    12961 tcctcaagca gcgcgccgag gcgctggacg ccctcgcgtg cggtcttgac atacggcagc
    13021 acgatggtca ggaagtcctc ttcgccgatg ccgccgggca cctcgggctc atcgcccttg
    13081 gccggcgtgt attccacgaa cgggtcggcg ccgagcacgc gctcgttggt ggtcagggtt
    13141 tcggtggcgc tcatcgccac gttcgcctcg ttgacgccgg cctcgcccca aataccttcc
    13201 ttgaggtccg cgttcggcac ggcggtgtac tgcagcgggt cgtccggcag ggtgatggtc
    13261 aggtgggaga tcaccgactt gtattcgcgc ggctgttcct ccggcttcac gacgatgaag
    13321 cgcttcgggt tgaactcgcc gttcgcggaa tcctcgttgc gggcgatgat ggtcgagccg
    13381 tcatagctcg cgtctttacc taccagaatc gtggtgcagg ccatagtgtg atggtgtcct
    13441 ttctttgttg tggtcacgct cccactggcg ggagctggac gacgaagtcg gactgaaggt
    13501 ggttttgtac tgacccaccc ccagtcagtg atgctgacag cccccgcctg cgggggctca
    13561 tgtggctcac gccttgccgg tcaggaagat gccgtcggtg tcgggcactt caccgctgtt
    13621 gcgcagcgcg tgcaacagct cgttgagcca gaagccctga ttcaagccgg gcaacggctt
    13681 cttggtggcc cacacctgca ggtagtactc atgatccttg tccggcggct gcggaccgtt
    13741 gtaccgcatg atcaccgccg ggtcagtgct gcggcccaag aacttggagg ccgaggagtt
    13801 gcacccctgc acggtctcgg gaatcatggc cggcagctga cgggagaagt ccggcgggat
    13861 ggccaacgca tgcgaatcat tgaagtcgta catcagcgca tcaatcggca gattggccac
    13921 ggaccagtga atccactcga agccgcagac cggaatcgaa tccgggtcca cgaactccca
    13981 gtgcaggtag cgggcctcgg gatccacatc atcgatgtag aacgggaagg agaccacagg
    14041 cacgccacca ttgaaatatt caggggccgc agccttggcg aaatcgtccg ggatgacggt
    14101 gaaatcggcg gaaatcttca tacgtcccag tatcgctcat atgtgtgacg tactgggggc
    14161 tgtgcgttac ggggtctctc ccccagtctc gctgcgctcg acagccccca ctcatcagag
    14221 gggggctagt tgcgtcgggt tatgagatca gggcgcagaa gagggactcg aataccagta
    14281 gcgggttgcc gttgccggct aggcgcttgc gggcgtgggc cacttcgtcc agacgggtca
    14341 cggcaccggc ccggttcaat cgcaccgaca attcggtgat cgacgagcgg ttctccaggt
    14401 tgatgaggcc caccgaatcc tccgcattgt tctgcagcac cgccacatct cggtagatgg
    14461 aagcgatgga gttcagcgca cgatccaata cgtcgcgcgt acggcgggtg accaaacgct
    14521 tcacatcgtc tttcttggca atctggttga atgcgccacg cagtttcggc gggatgcggt
    14581 cgctcggtgc cagtccgttg atgcggcgga attccgcttc ctggccggcg gtgatgcggt
    14641 tcgcgtccgc ctcggcctgg gccttggcat tgtcgataag attgccggcc aacaacactg
    14701 catccgaggc acgggccaga ttcagcacac cgaccacaag ttcatcgcgg tcggacataa
    14761 cccgttcgtc ggtggcgtac aacttggcga tgccgatatg gccttcggcg agtcgggccg
    14821 cacgctgcgc cacttttggc tcgacattgg tggtggaggt caggaatccg gccaccgcct
    14881 gggtggaggg cacggcgagg ttcacgatgc gtgtgcgcga gcggatggtg ggcagcacgt
    14941 cctgggcgct gggcgcgcac agtagccaga tgcaatgctc ggccggctcc tcaatctctt
    15001 tcaagagcac gttggtggtg cgctcaagca tgcgatccac gtcttcgatg atgatgatgc
    15061 gccacggcgc ggtggcgggc atctgctcgg aggtagtgat gatctcacgc acctgatcga
    15121 taccaatagt caccttgttc gtggtcagca ccgtgacatc cggatgcgtg ccggcaagca
    15181 cctgctgggt gacgcgcgtg ggctcagcgc tcatgccgtg atccgggctt tccagcgccg
    15241 ccgcgaatgc tcgggccatg ttggaacggc ccgatccggg aggtccacag atcagccacg
    15301 actgggtgat ctggctcggg tcgccctgcg cgatacgact gagcatgtcg attaccggct
    15361 tttggccgac tagtgaatcc catacgctca tgcgagctcc ttgatgtcgg attcgatggc
    15421 agcccatacc gcttcaatgc tctggctcgc atcgataacg cggaaacgat tcggctcggc
    15481 ggcggccaag tccaagaagg cctggcgggt gcgggactgg aagtcatcac cggccgattc
    15541 catacggtct tccgcgtgtt cgaggcgatg gtgggacagg gctgggtcca tatccagcaa
    15601 atacgtgcgg tcgggcagca ggttgttggt ggcccacatg cttaaggatc gaatctcttc
    15661 tggcgtgagt tcgcggccac cggcctgata ggccaaggaa gagtctaaat agcggtcggt
    15721 gatcaccact tcaccgcgct ccaatgccgg gcggatggtt tcggcaacgt gctgggctcg
    15781 gtctgcagcg aatagcagcg cctcggcgcg cggtgcgatg tcgactgcgc ccccatccac
    15841 accgtgcaac agcagttggc gaatggcctt gcctaacgca gtgcctcccg gctcacgggt
    15901 aacgaccacg gtacgcccct gagcctcaag atacgcatgc agccgctcga cttgcgtggt
    15961 cttgcccacg ccatcgacac cttcaaacga aacaaacaga ccgctcatgc tattgacctt
    16021 agcgttaggc gcagagttgc gcccaacagg cagttaaacc agctctacct ccgtttcaag
    16081 gggctgattt ggtcttttct gaaggtctac ttccgtttcg aggggctgtt gcccaagtag
    16141 acaacgttcc aaaacggcgg aatgacgttg gcgcggagcc gttcatactc gtggttcagc
    16201 agatgaccag ccccttgaaa cgcaagtaga tattctcaaa acgagaaatc agccccttga
    16261 aacgcaagta gagcaataat tccgctcagc cacgcttggg aattacggcg gggcgctcgt
    16321 agccctcgtt ggcggtcagc cagtcgacgt cctttttcac agtggcgggg gtggaaccga
    16381 gtttttcggc gatgcgctgg ttggaccagc ccttgttcgc caacttcttg acttcggcac
    16441 ggcgttcggc gcgcttggcc tcctgcgccg aaaccgcagc cgaggtaccg cccttcttgc
    16501 ccttgccggc aacggccttg gcgccaccag cacgcccagc accgcgacgg ccacgggtgg
    16561 agggaccagc cgcacgcttt tcggcgagca gtcggaaggc gtcatcaggg gtgatggatt
    16621 caggcgtgta ctgacgcggc accgtgcggt tggtctcgcc atcggtgatg tacgcgccga
    16681 aacggccgtc cttgatggtc acgttcttgc cggtgttcgg gtccttgccc aaatcacgca
    16741 gcggcggctt ggcggcacca cggccacgcc cgcggccgta cttgggctgc gagaacagtt
    16801 ctttggcctt gtcgatatcg accgtgaaga tctcgtcttc cgaggccagc gagcgggtct
    16861 ccgacttgcc gtcggcaccc gtcttggtca ggtaggggcc gtagcgaccg ttgttggcgg
    16921 ccaccgtgca ttcggatacc tcgccggttt cggcgttggt ttcctcgtat gtgcccactt
    16981 cgcgcggcag gctcagcagc tttagcgcgt cttccagcga cagcgattca gggctcatcg
    17041 tcttgaacaa cgaggccatc ttcgggcgcg acgcggcggc ggccgcggcc ttcttcgaac
    17101 ccttcttggc cgcagaagca ccagcagtat cgccagcagc gccagccgaa gcctcagccg
    17161 gcggcaccaa cgccacatac gggccgaaac gcccattgcg cacctcgacg gtaccgccgg
    17221 ataccggatc aacgcccagc tcgcgcggtc caccggagtg gttgtcgatc aaatcgcggg
    17281 ccacggccac cgtcagctca tccggcgcga tggtgtccgg cagagacgcg cgcttcgggt
    17341 tgccttcggc gtccagatgc tccatgtctt ccagatacgg gccgtaacgg ccgacgcgca
    17401 cgtgcaggcc gtcgccgatg tcgatggtgt tgatggcgcg cgcgtcgatc tcgccgagct
    17461 gcgcgacctg ctgttgcagg ccttcgtgcg cctcgtcggc gttgcgggcg gcgccctcgc
    17521 cggaaccgaa gtagaagtgg gtgagccagt cgcggccgga ttcctcgccg tgggcgatgc
    17581 ggtcaaggcc gttttccatc tcggcggtga actggtagtc gaccagcttg gggaagttcg
    17641 tctcgagcaa cttggtcacg gagaaggcga gccagctggg gatcagcgca cggccacgct
    17701 cgtatacgta accgcgatcc atgattgtgg agataatgct cgcgtaagtg gacgggcgac
    17761 cgatctcctt ggcttccagc gtcttgacca gcgtggcctc ggtgtagcgg gccggcggct
    17821 gggtttcatg gccgtccggt tcgatgtcgc ttgcctcgac ctgctggccg acgctcatcg
    17881 gcggcaggga ggtgttggat tcggaggcgt cacccctgac ggccttggcg tccgtcttgc
    17941 cggcggcagc agcctggtcc gagccagccc cagcgccggg agcggcggcc ttgggcttgc
    18001 gcccctcccc cgtggccttc ataaagccgg ggaattcgat gacagtgccg gaagcctgga
    18061 acacggcctc gccctcggtg gggccggccg gcgcagacag gcgcacggtg gcggtggaac
    18121 cggtggcgtc agccatctgg caggcaagcg tgcgctgcca gatcagcgtg tataggcgca
    18181 gctggtcgcc gggaagtttg gaagccagct cgtccgggtc gtggaagcgc gagcccgccg
    18241 ggcgaataca ctcgtgggct tcctgcgcgc cagcggtttt ggtggcgtac tgcttgggct
    18301 cggcggagag gaacttgtcg ccgaaatggt aggagacggc gttgcgggcg gcggtgaggc
    18361 cctcctgcga cagcgtcacc gaatcggttc gcatataggt gatgtagccg ttctcgtaca
    18421 ggctttgggc cgcgcgcatc acggcgcgag cacccatgcc gaggcgattg ccggcggtct
    18481 gctgcagggt cgaggtggtg aacggcggct gcgggcggcg gcggtacggc ttggtttcca
    18541 tcgacatcac agtgaaatcg gcggtcttca gcgccgaagc cacggcggtg gcaccagcct
    18601 catcaagctg gcgaacgttc gccgcgaacc cgtccggggt cagctggccg tcggcaccga
    18661 aatccttgga gccggcgagt cgcttgccgc cgagcgcgat catgcgggcg gagaattcgg
    18721 cacgctcccc cagcgcgtca ggcgcggaca gcgtggcgac gatatcccag tacggcgcgc
    18781 gcttgaacgc catgcgttcg cgctcgcgtt ccacgatcag gcgggtggcc acggactgca
    18841 cgcggccggc ggacaggcct gggcccacct tgcgccacag caccggcgac agctcatagc
    18901 cgtacagacg gtcgaggatg cggcgggtct cctgtgcgtc gaccatattg ccatccacat
    18961 cgcgcgtgtt gttcagggag gcgttgatgg cgttcttggt gatctcgtgg aacaccatgc
    19021 gcttgaccgg cacctcgggt ttcaaggtct gcaccaagtg ccacgcgatg gcctcgccct
    19081 cgcgatcctc atcagtcgcc agatagagtt catcggcgtt cttcagggcc gatttgagtt
    19141 cgctgaccgt acgcttcttg tcgccgtcga caatgtaata cggcttgaac ccgtcgttga
    19201 cgtccacgcc gaacttgccg aacttggcct tgtctgccgc gggcacctgg ctgggctgcg
    19261 cgaggtctcg gatatggccg accgacgcca tgacggtgta ccccttgccc aggtagtcac
    19321 cgatcttgtg ggccttggtc ggagactcca caatcaccag tttggtagcc atacccactc
    19381 cttatgtatt gcgcgaaaca cacgacgttt ctggcgtcat aatactcatg aatcatgtcc
    19441 aactgccaaa ttccgccgtt gtgccgggca aattcggccg ttcgcacccc atgttttacc
    19501 gtttgggcgg tgccggcgga gcgccgatca gacccagccg gcttaagggc gctgcagtga
    19561 tctgacgcag ggccagcaac agtccgaatg cgagcatggc cacacccgca atgcccacaa
    19621 cagcgctgac attcgccccc tgaaccgccc agctggcgct gacattgagc cccggcacca
    19681 cctgccagat cacataaccg ctgtaggcga gcaacagcag gcccccggta atcagcacgc
    19741 tgccgacgat ttgcacgctc actgaggaga cgcggcgacc cggctcgtcc atgccggact
    19801 tgcgcgtaac tgccagagcg agcagggcca atcccgccac cagcaggatg gacatcgcca
    19861 catccgaagg gcgatgccat tgtccgtata tcacggagac accgacggtc accgcccaaa
    19921 ccacgccgat gacgcccgcc aaagctcgaa ccgcacgagg cacggtaagc agcagaatca
    19981 ccgcacaggc tgcggccagc atcgtgtgac cggaaggcgc ggaattagcg gcaggagatt
    20041 cagtgttgat gatgaacgga cgcggcaaca gacccttgat acgggtgacg gccaagcaga
    20101 ccacggcgaa cacggcgatt tgcccggcca accaccagcg gcgtcgaatc agcgccgtaa
    20161 taatgccgat tgcggcaatt gtgcagctga cagcgataac cagccaggat tgtgcgacgg
    20221 cgttcatcac gccggtggcc caggacggta catcgttgtg caggtttttc cagaccagtt
    20281 cgtcgtacga ttgtccgttt tcggtaaaca cgcaccccca ccacacgccg aagccgatag
    20341 cgatcaggag cagaccgaat accacgcaca gtacgcggct ggagattcgc gggcgcacgg
    20401 taagcgggtc aagccgggcc aagccgcggt cgatatcaga tgccccatcc gcatcgtcca
    20461 tgtccagcgg cctgaaatcc cgtaaggagc ctgatgtgga taccgatgca tctgaaccgg
    20521 agaccgggcc gagcgcagcc aatgggtcaa caacgttcga cgacgagtca ggctgtgctg
    20581 cgcctgagcc gaccagattg accgccgcat gcgggtcgcg aattctaggg gtctgtgcgt
    20641 cattcatacg ttcgactgta cggccgcgcc ccggacaggg gaccacgcta gtgccagctc
    20701 acagccaaat ccgcacataa agaaaacctc cacgcacccg tcagaggcca cttacccttg
    20761 ctgcattcct gccctggggg agttgggtga catagcgccg cgtggaggct ggggataagt
    20821 ctatggagac gtatggacca gcgcagcgac acgaaacgtg cgcgccgggt cgcacgacag
    20881 gccaaccagc gcattcaggc aatattcatg cagcatcaag aagcgtgccc aattgtaaga
    20941 attccggaaa aataccggca taaacgcgtg tggctacgct cacgtcaaac gattgttcaa
    21001 ggccaacagt tacggtggac accatggctt cttatactcg tcgtgcacat tcgggaggca
    21061 atggccccgc tctgaggcaa ccgcgcaccg cgcaaagcag agccggccac tccgcacagg
    21121 agggccccca tacgccccag gagagccgtt cccgccacgc aagccacaag gcttccaata
    21181 gccatcgagc cccgaacagg aagaaaaaga acacaggaaa aggcaagcac cgtgtactga
    21241 aatgggtgct gggcatcttc ttctccctga ttggactggg cttgctggcc ggtatcgcag
    21301 tattcgcata cctgtacacc accactgagg tcccgcaacc cgagaagttc gcgttggccg
    21361 agaaaaccac cgtgtattac gcggacggca ccaccgcgat cggctcctac gcggagcaga
    21421 atcgtgaaat catctcttgc gaggggctgc cggactacat cggcaacgcg attgtggcgt
    21481 ccgaaaaccg tacgttctac acggataaag gccttgatct gaagggcatc gcccgcgcat
    21541 tcattaataa tgtgaccaag ggcacgcgcc aaggtggttc caccatcact cagcagtatg
    21601 ccgagcggta ttacatgggc gaaaccacat cctatgtggg caaggcgcgc gaggccatca
    21661 tggccatcaa aatcgcgcag accgaatcca aggacgaggt gctgtgcaat tacatgaaca
    21721 ccatctatct gggccgcaac tcctacggta tccaggcagc ggccaaggca tacttcaata
    21781 aggacgccaa ggatctgacg ctgtctgagg cggcgatgat cgccggcatt attccgtcac
    21841 cgtccacgtg ggatccggcc gataacgccg atatggccaa atcgcgcttc aagcgtgtgc
    21901 tgaacatcat gcaggaggat ggttacatca ctgccaagca gcacactgat gccaagttcc
    21961 cgcagaccgc cgcggtggcg cagcagaacg aatacgcggg gccgaacggt tatctgctgg
    22021 atatggtgcg ccgcgagctg gtgcagtcca aggcctttac caaagaggat ttggatactg
    22081 gcggatacaa gatcatcacc accatcgaca agagcaagca ggatcttatg cagtccatcg
    22141 gcgacacccg tctggacgac atgcctgaat ccctgcaaat cggcggcata gcgctggacc
    22201 cgaagaccgg tgaagtattg tccgtatacg ccggttcaga ttatctgtcc aagcagctca
    22261 acaatgccga tcaggccgta ttcgaaccgg gttcgactat gaagccgttc gctttgctgg
    22321 gggccgcgca atccggcgtc agcttcgata cgttgttcaa cggcaattcc caccagcact
    22381 tcaccggctt ggatcaagag gtgaacaacg ctctggagaa caactggggc aacatcaatc
    22441 tgtatcaggc caccgccaac tcggtgaaca cggtgttcat gaacgtcaat gagcatctga
    22501 cgccgaagcg cacggccgca atcgcccatg aagcgggcat tcagggcgat atcgatgaaa
    22561 actccatgta caacgtgctg ggcatcaacg ccctgaccgt gtgggatttg gcgcaaggcc
    22621 attcgaccat cgccaacaat ggcgtgaaga acaccttgca catggtgtcc aaagtgcttg
    22681 attccaacaa taaggacatg tacaacgcgc ccagcaacgg caccaaggtg ttcgatgcca
    22741 acgactgcgc tttggtacag aaggccatgc agggcaccac cacgtacggt acggcggccg
    22801 gcacatccag catgttgggc cggcccgtcg ccggcaagtc cggtaccgcc aacgacgaaa
    22861 tggcttcgtc gttcgtggga tacaccccca gcatgatgaa tgtgtgggct atctggaatc
    22921 cggacgataa gggcaatccg caggtggtgc cagccttctc cggatacggc gtctcttcca
    22981 ctggataccc cgcccacctg ttccaggagt tcatggctca ggccttgcaa ggcaccgagg
    23041 cggagcagtt cccgaccgct aaagacaacg gcaagatcgg cggctccgac ggcacttggg
    23101 gcttgggcaa gggacagagc agcggcctgt ccaacaacac caacaagcag tccaccgaag
    23161 acgcgcagaa gcaggctgag caggacgcac agcagaaggc ggccgaagag gaagccaaga
    23221 agcaacagca ggctcaggag ttcgcccagc agtgcctcgc caatccgagc tattccaccg
    23281 agtgcccgaa ttatcccggc accactaccg gcggagatgg caacgccggc aacaataccg
    23341 gcggtgacaa cgggaacaat acgataatgg taattccgga ggcaccggcg ggaacaccgg
    23401 caccaacggc gtcacccagt agtaagtaaa acacagacaa caaggctccc tctgatgcaa
    23461 tcagagggag ccttgttttt gctctgctag cgctcagcgc ttgcgctgga aacggttgat
    23521 ggcggagata atcgacttca gggcgctagt ggtaatcgac gaatcgatgc cgacgcccca
    23581 cacgatctgc gcgtcatcgg cctcgccgac ctggcattcc acgtacgagg cggccatggc
    23641 gtcggtgccg acggacatgg tgtgctccac gtaatccata atcgaggcgt caacgccaaa
    23701 gttggaaatg gcgttgagga aagcggcgat cggaccgttg ccgatgccgg agacctcgcg
    23761 ctccacgggc gcatcgaccc nnnnnnnnnn cggtcgagta gacgagcctt gagcacggtg
    23821 tcggagccat cctcaccgga gctgacggcc accttgagca gcttcaagcg gccccacggc
    23881 acgagcgagg cgtcatgagt atcgcccaca accacaccgg cggcggtcat gccggactgt
    23941 tcgaccggca ggtactcgtc cttgaacaga cgccagatat cctcgtcctt gacttccttc
    24001 ttggtcgtgt cagcgtagtt ctgcacgatc ttgtcgaatt cgatctgcag acgcttgggc
    24061 aggtcaagat tgtggttggt cttcagcagg taggccatgc ctcccttgcc ggactgcgag
    24121 nnnnnnnnnn gccacacgaa gctgtcgaga tcggcgccag cacgctcggc ggccacctga
    24181 cgagcctcga gacccttctt gatggcgtcc tgatgcgagc cggagaacgc agtgaacacg
    24241 aagttgccgg cgtacgggtg acgctcggaa atcttgatct ggttgcagta ctcaaccgtc
    24301 ttgcgaatct cgggcacgtt ggacaggtca agctgcgggt cgatgccttg ggtgagccag
    24361 ttgaggccca gcgtgaccaa gtcgacgttg ccggtacgct cgccgttgcc cagcaggcag
    24421 ccttccacgc ggtcggcgcc ggccagcacg gccagctcgg tggcggccac gcccatgcct
    24481 tcgtcgttgt gcgggtggag ggagagcacc acggagtcac ggtcgtcgag gtgggtggag
    24541 acgtactcca cttcgtcggc gaacacgttc ggcgtggtca tttccacggt ggcgggcagg
    24601 ttgatgatca tcgggtgctc gggagtcggc ttgataacac cgatcacggc gttgcacacc
    24661 tcgacggcgt actccggctc ggtgccggtg aaggattccg gcgagtactc gtagtacagg
    24721 tcgatgccct tggcctcgtt ctcgaggtcc ttgcacagtt cggcggcgtc ggtggcgagc
    24781 ttcttgatct cctccttgtt cttgcggaac acgacctcgc gctgcaacac agacacggag
    24841 ttgtagaagt gcacgatggc gcgcttggca cccttgagcg cctcgtaagt gcggcggatg
    24901 aggtgctcgc ggcactgggt gagtaccacg atggtcacgt cgtccggaat cagctcacgc
    24961 tcgatgagca tacggatgaa gtcgaaatcg gtctcggaag cggacgggaa gcccacctcg
    25021 atctccttga atcccatgga gacgagcagg ttccagaaac gcagcttgcg ctcggaatcc
    25081 atcgggttga cgagggcctg attgccgtcg cgaagatcga cggagcacca gcgcggcgcg
    25141 cgctgcagtc gcttgcccgg ccacgtacgc tccgggtaat caaacgggat ctgcttgtcg
    25201 taggcggcgt acttgttgta cggcatacga ctcggcttct gcggatcgcc aatgaatcgc
    25261 gccggaggca gcagcgggtc gttgttcccc ccattggacg ctgcggctac cgctgcgaga
    25321 tcaaaaacgg atgattgatc ctgacccatt gttcctcctt tactgtgtga accattcata
    25381 caatcgctta ttggatattc gtttacgcat tggtaacatg cataacttcc tctatcctag
    25441 cgacggccca ttccaacatc ccgttcatct cacaatacgc cagcacactt agcgttccga
    25501 cgcagcacta ctacttacag ctatgcgtgg gggcgcatgg gcgacgtcct cgcgttcttc
    25561 gactgggagc acagcgcgaa cgggcccacc gaggccatca acggaaggct cgagaccctc
    25621 aggggcatag ccctgggatt ccgcaaacct cgacaactac atcaccagaa gcctgctgca
    25681 cgccggcggc ttcagacacg ccatccaaac ccacctctga cacatgcccc tacacgccaa
    25741 aatcggaaga gcccgataac ctgctatacg acgaggatgc cacctattcc gcattcaggc
    25801 cgtatcagca gaattgatgt attgaccggt tttatggtcg tcaccgcttc ttcaccaacg
    25861 agcggatgac ggccataaaa ccgaggctca tggcaccgga cagcactgcg aatacctgcg
    25921 ggcggcgcaa ttcgttacgc atgctgatgg caccggtcac gcagtggtcc tcgaaccaga
    25981 cgcgcacctc gttgctggag tatcggccca ccagatcacc ccaggtgagc atgcgggtca
    26041 cgtagccggc cgggttgctt tcatggaatt cgaacaggcg gatgccgcga tagcgcgact
    26101 tgggaccata gcattcgaat ccgcaggtag gcgcgtagcc caaatcaatg ccatgcgcgt
    26161 ggcccacgaa ggcgttcttg tgatcgtgac cgcagaacag cgcgaagtag ccgcctgcct
    26221 cacgcaaggc gtccacttca ccgacgttct cgtccgcaca gccaatcgcc tcacccagcc
    26281 gcgatcccgg ccggcaaatc tcatggttga gcacgtagca atggccggcg tgggtacgtg
    26341 cgccttccac cgcgttcggc gtccatgcgg gaacctcttt gaggcaatcg tagaattcct
    26401 gcggcggaat gtgctggaag gcgatggcag gcaccggctt accgtcgccg ttgcgttcgc
    26461 caagttcgga ctgtacggct cccagccaat cgatcgcctc ggggcttggc gtgccatacc
    26521 catcggaatc agccaaatca agaccgcgcg gattgaccac ataggccggg tattgggcgt
    26581 cgttctcttc aggcttgccg gcgtaatcgc cggaattcac catcatgacg gacatagcta
    26641 cgcgcccgga tccgtcggaa gcctcaatgg gcagggcaaa agtgcctggc tcgaggctgt
    26701 cgaccgggtt caggcagccg gggaattcgc ggtagatgcc gtcctgctcg tcggccagga
    26761 tgccgcactg gaaatcgtga ttgccgtagg tggcggcgaa cggcacttcc gcttcgatga
    26821 ccgggccaag gaagccggca aacgtgcggc ggactttgtc acgggtctcg tccatgagct
    26881 cgtccaaagt cgtcggcaca tcctgtggat cggatgttgt ggagtagacc agcgtggacg
    26941 tggtggacgc ggtggacgcg gtttgttgga cgtcgggatt gctgccgtca gacttcatgg
    27001 ccagcagacg attgttcacg cggtttctgg cccgccgctt gattccgcgc agcttggcct
    27061 cgacttcggt gaccgcacgc acgcgagcgc ccggttcctc gccacggcgg cgcaggaaag
    27121 tgtcgatgta ggcaggatcg taaccgcgaa tctggtcgcc ggtgaacacc accagatccg
    27181 ggtcggcttt gcgaatggcc tcacggataa ggcggatggt atcggggagt acgttggggc
    27241 cgtcttggat gtcggccatc tgcagcacgc ggaagctgcc gtcttcgcgg aatcgcaggt
    27301 tcgtcatgcc tcaccagtct agggacgtaa tgctgtttcg cacagcatac gcgccggtcc
    27361 ttcacgttcc ccgccgctcc gcacggcagc tgaatcacta cgagaaatcg tagaacgtgt
    27421 atcccttatc cgctccgctc gtcacgttga gcaatgcttt gccgttcagc gtatcgacgt
    27481 cgtttcctat gagatagccg tctcccgtgg ctcccatatc cgagtcgtgg taggagccgg
    27541 agaaggtgcc atccttattg actttcatta cggttcccca tgcgccgaca acgggcggtc
    27601 gccatccacc ttgccgacct tgtcttttga ggagaatcgc tttaacggaa cagactcacc
    27661 ccgcgacggg ccgtgcaata cgcgttgccc agccaagtat ggcgggaatt gggccagacg
    27721 gctcccagtc gcggtgcgtt ctggctcatc cagatgctcg gcacgcggcc gtctgcggag
    27781 cgtgagccga gcagattgaa gccgttcttt tccagcagcc actccgggtg ctgggtggcg
    27841 atggccaagc cttcctcgag ggtgagcggc aggcgctcat cggagtcaat aagtttgcgg
    27901 gccacatccg gctcgcgatt gacatagcac gtgccggtgt gcggattgac gaccagatag
    27961 aacgggcctt ccggcggcat gaagccgtct tgaagcagga agctggcgat gtcacgcggc
    28021 ggcatggtgg tgaagcctgc catgcgattg atggaggttc gtgcaatcag cgactcaggc
    28081 gaaaccagtt cacgcgtggg caccagcagg atgttcgatc ccaaatcgga atgctcgagc
    28141 gccccgatca gaggtcgggc cagcgagcgg aatgcggcgg cggaaatatc cgccacatcc
    28201 gggtagccga gagccacgat acggtcgagc tgtttttggg cctctactga tgcgatagac
    28261 atggctacca gtgtagaaca tcgccttcgc ttcattgggc agccaccatc tgggaagacc
    28321 actgccgttg ttccgcgtgt ttcgtacaat tgcattgttt tacggccacc cgcatgacgg
    28381 ccgtccgacg taagtaatcc atcgaaaccc agaggtcata gaggtcatca tgtccgaaaa
    28441 attgaaggtc ggcatccttg gtgccaccgg tatggtcggt cagcgtttta tcacgctgct
    28501 tgagaatcac ccgtggttcg aggtggtcac gcttgccgct tccgcgcaca gcgccggcaa
    28561 gacgtatgcc gaggctgtcg gcgaccgttg gaagatggaa accccgatgc ccgaatatgt
    28621 caagaacatg gtggtcgtcg atggcgacga tgccgagtcc gtggcctctg gcgttgattt
    28681 catgttctct gcggtaaaca tgccgaagga ccagattcgc gccattgaag agcgttatgc
    28741 caagaccgag accccggtgg tctccaacaa cagcgcccac cgctggaccc cggacgtgcc
    28801 gatggtcgtg ccggagatca acccggagca cttcgaggtg atcgagcacc agcgcaagcg
    28861 cctgggcacc actcgtggtt ttatcgccgt caagccgaac tgctcgattc aggcctacac
    28921 gccggcgctg gccgcgtgga aggaattcga gccgcgcgag gtggttgtga gcacctacca
    28981 ggcgatttcc ggcgcgggca agaccttcaa ggactggccc gagatggtgg gcaacatcat
    29041 cccgttcatc tccggtgagg aagcaaagtc tgagaaggaa ccgctcaagg tgttcggcca
    29101 tgtggacgcc gccaagggcg agatcgtgcc gttcgacggc gacctcaaga tcacttccca
    29161 gtgcatccgc gtgcccgtgc tcaacggcca caccgccacc gtgttcctga acttcggcaa
    29221 gaaggccacc aaggaagagc tcatcgaccg tctggtgaac tacacgtcca aggcttccga
    29281 gctggagctg ccgcacgcac cgaagcactt catccagtac ctcaccgagg atgaccgccc
    29341 gcaggtcaag ctcgacgtgg actacgaggg tggcatgggt gtttccatcg gccgtctgcg
    29401 tgaggattcc atcttcgatt ggaagttcgt gggtctggcc cacaacaccc tgcgtggtgc
    29461 agccggcggt gcccttgaat gcgccgaaat gctcaaggct ctcggctaca tcaccaagaa
    29521 gtagttgaaa ctgagtccga aactgagaaa gccctccgca aggagggctt tcgtcgtata
    29581 taccgcaaaa acgcggctca gtggctcagc ggccagtacc gccgtacacc acggcttcga
    29641 tttgatcggc atcgagaccg tatgcggtgt gcagcgcctt gacggcgtcg ttgagctgat
    29701 cgagcggcac gagcgcggcg atacgaatct cggaggtaga aatcatcagc acgttgatgc
    29761 cctcatcgct cagggcctgg aagaacttgg cggccagacc ggcatgggtc ttcatgccaa
    29821 cgcccaccac ggccaccttg cctacgttgg tgtccacatc gaaggagtgg tagcccagca
    29881 cttcctgctt gtcgagcagt gtgttctgaa cctgcttgac ggccgattcg ggcacggtga
    29941 aggagatgtc cgcagtgccg acggatgcgc cggcctgcac gatcatgtcc acgttcacgc
    30001 cggcggtggc cagctcggtg aacaccttgg cggccatacc cggctcgttc ggcacgccac
    30061 gcacggtggc caacgcctcg gaacgatcgt gggcaatgcc ggaaatcacc ggtgcctcgg
    30121 ggcccaggtc agggaacagg tcgcccatgg atttcgcttc ttcttcagtc atgtcttgcc
    30181 ttgtcttgct tgaaattact tacgattatc gttcgccgta gccgcgctca gtcgaggttc
    30241 ggcagcgtac gcgggtcaat gccgtcgggc accaccaatg tgccggggcg acgggagaac
    30301 gaggaacgca cgtgcagcgg catgttgaaa cgctgagcgt actccacaca gcgcagtgcc
    30361 agcaccttgg agccgcagga ggccatctcc agaatggact cataatcgat ggacggaatg
    30421 cggcgggcgg aaggcacgat acgcgggtcg gcggtgaaga tgccgtccac gtcggtgtag
    30481 atctcgcaga tatcggcacc gagcgccacg gcaagcgcca ctgcggaggt atccgaacca
    30541 ccgcggccga gcgtggtggc gtcacccttg gcgttgatgc cctggaagcc ggcaacgatg
    30601 gcgatgtctc ccaatgagag cgcgttcttc acgcggtccg gtctcacggc cttgatgtgg
    30661 gccgcgccgt aacgggcgtc ggtgaagaag ccggcctgct gaccggtgaa ggagtgggca
    30721 cggctgcctt cggcgtgaat ggccatggcc agcaggctca tggaaatacg ctcgcctgcg
    30781 gtcatcagca tgtccatctc gcgctcgggc gggttggagt cgatgctcag cgcctgatcg
    30841 ataaggtcat cggtggtgtc gcccatggcg gagaccacca cggccacctt gttgcccttc
    30901 ttttccgtct caacaacgcg cttggccaca cgtttgatcg attcggtatc ggcgaccgac
    30961 gaaccgccgt acttctgcac gatgagagcc actgctgtcc tttgttcttc tcttagggga
    31021 gtttgctcga tattctacgc aaacgcaacg attataaagg ttgccgcgtt caccccgcga
    31081 ccccgcgccg acatgtggac acgggcatgg cctacgttgt tcagcaaata ttcaagtttt
    31141 caccggcgat tctttgaggc ggccgcatcc atcctttgag attcgagcgg ttaactatca
    31201 attaccggca aacgaaagca aaccggcgag gtttccgcag aaccccataa ctgaatacct
    31261 tcttcttcct ctctcctttt caaggcccgg tcccgcagcc cgggcctttt ccttttgtgt
    31321 tgggaacgat gcgtcaaacg cacgatacga gttcacggag caagcgtggc caacggcact
    31381 accaataccc catcatcccg gcgataagcg gcagccgtag cggtcagcac catcaagaat
    31441 gacggacggc cttcgtgatc tttatcaatt ctgtcagcca actttttcaa atgggccgcc
    31501 gcaatatcaa cctgcttttc tccgagcttg acctcaatga gagcccatcg tccatcatgc
    31561 aggccaatga ccgcatcaga ttccaatccc gtcttatccc gataatggaa cacatctccg
    31621 ccaagcgcat cagcatacac acgcagatcg cgaatacaca tggactcaaa cagcaagcca
    31681 aaggtctcga aatcattaag gagtttctcc ggggtcgcgt gcagtaccgc acaagctatc
    31741 gacgggtcgc tgaagtgtct ggttgggctc gttctcactg cagtcttaga acgtaaacga
    31801 gggttccaag ctggaagatc ttcagtcacg caagctcgcg acaatgcatc taaataatca
    31861 gagagtgtac cttccgaagg cggttcaccc tgcatatccg ccgcgatagt ggattgcgac
    31921 gcctgagaag aaatattgcg cgcatacgag cgcataatca gacgcatcca agttttattg
    31981 cgctttatgc catccatacg attgatgtca gaatcaagta gttcttcgac ataatccgcg
    32041 gccatgcgca aggcaacatt tactcgagat tcgactactg cttcaggcca gccaccgcgg
    32101 catagcgcaa acgcggtgtt ttcaatatcg aaatcggaga aacccgaaac atcatggttg
    32161 ccatcgaaca actctcccaa cgaaacaacg cccgtcgatt cctgcgattc aaataaggac
    32221 atggtacgca tggttaatcg ggcaattctt cccactccgg aatgagctgg ttcctgttga
    32281 ggagtcgccg atccagttag gatgaatcgg ccacgaccgc gccctcgatc aatggcgaaa
    32341 cgcaccgcgt cccacaattg cggggccatc tgccattcat caatgagtct cggctcctca
    32401 cctcgcagga tgagagaagg ctttgtatca gccaaggcca acagcgtagc actgcgatcc
    32461 gggtcctgca tgaagacctg actcgccgac tgcctttctg ccgttgcagt cttgccacac
    32521 cattttggac ctttaatctg aacggcacca gaagactgca atgcccgagt cagcaaccca
    32581 tcagccactc gaggaaggta ctcgccacgt tgaatctccg agctctcccc tgccatatgt
    32641 gccctcccgc aaaccgtcga aacatcaacc tataaatcac tctattgcgg aagttggacc
    32701 attctaattg tggaagttgg accattttcg ttgcggaagt tggaccattc ctattaggga
    32761 agttggacac tagattacga actagacttt tcctttttgc agcaacagtg ccaccaacac
    32821 gatgggcgtc atcaccgcct atcgccagac ccttttcctt gacctaaaaa gatgagatgc
    32881 ccagacagtg ccatcttttc tctgagcaca ctagccaatt cggaacgcag cggcgaaccc
    32941 gtgatccgcg catcctctgc aaccgtgcct ttgacgttcg gcaatccggc gaatgagctt
    33001 ttgctcaggc gttcttcgcg attcatttct ttggtcggaa aacagaatac gcgggaacgc
    33061 ccctcacgct tacgaaacca acggctcgac gacgaaccga catccacgaa aacagcaata
    33121 gcgcctcttt ttatcggggt aacatattcc ctctttatgc attcataccc ccctatttga
    33181 aagctctgcc aaatattgcc gacgccgcga cttatcatgt tgccatactt tgcaatcctt
    33241 tgcagcccct tgcatctcaa cggcttgtcc gaagtaagcc acatcgctga cggccgtagc
    33301 aacgttgttc gtatcgcatt tttcagactt cttatgcccc cctatattgc ccccatatgg
    33361 cccccgtcgg catgttgcat gaaaggcttg gcacggagta acgtttcgca gtgtggcgcg
    33421 tgcggtgttg ccatttgcga cagcacctat gccacgcacg actaagacag acccggccgt
    33481 cttgtataag caaatcatga gcagatcaaa cggaggaaca ggcgactatg gcattgctgc
    33541 atcgacgttc gacgagtaag cgtgcctcac agaaaatgcg ggaaaacaac gcggtcatgc
    33601 cggatgacac atctgcaaat gacaacgttt cctcagcgcg ccccgagccc acagcattcg
    33661 ttccaactcc atttgacgaa gtcatcgata tcagcgatct cgtggccgag cgccgacgcc
    33721 tgcagcgcaa tctgtgggcg atgcgcatca tcaccattgt gttgctgatc gccgcaatcg
    33781 ccgtggcctg cttcccgcta gcgttgcaat tcgagtccga ccgcaacttg gccgccacca
    33841 cagccaccac ggccaaagaa gtagctggct ggccttaccc gcaggcggaa gacaagctca
    33901 cggcggcccg cgcatacaac aagaagctcg ctgaatccgg ccagcctatc ttgggtgaag
    33961 ccgtcgatcc gttcgccgcc gctcaagggg gatcccaagc cagcggcgag gattccgcct
    34021 caaagaaaga caaggaatac cagtcactgc ttaataccgg caacggcgtg atgggcacaa
    34081 tcaaggtgcc caagcagtcc atcaatctgc cgttttatca cggcacttcc gaggaagcac
    34141 tggcttctgg tgccggccat ttgtacggca cgtcgctgcc agtgggaggt aaatccaccc
    34201 attcggtcat taccgggcac cgcgggttgg ttgaagcgct gatgttcacc cgtttggacg
    34261 aggtgaaaga gggcgacttc ttctacatcg aagtcatggg cgaaactttg ggctacaaag
    34321 tggaccgcat ttcggtgatt ttgccggatg acacgtcgaa gctcaagatc gtgccgggtg
    34381 aggatcgcgt cactttgatg acctgtacgc catacggcgt gaacacgcac cgactgctta
    34441 tctccgggca ccgcgtggcg attccgatgc cggcccccga gccgaatgac gtgcttgacg
    34501 cgcgcaatat cgcccttggt gttggtctag gcatactggc ggcaggactg ttcatcatct
    34561 ggttggcgcg acggcacaag gccgcgcgca tcatccgcca cggcgcattc tggccgtgga
    34621 tgagccgcaa gggcgagagc gccaatgata cgctggctta ggcctcgcgc cgaccggcca
    34681 gtgccctgcc cagcgtgatt tcgtcggcat actccaagtc ggagcccacc ggcaggccac
    34741 tggcaagtcg ggtgaccttg acacctacgg gtcgcaatag ctgggtcaag tagctggtgg
    34801 tggcctcgcc ttcaatattc gggtcgagcg ccataatcac ttccttgacc tcatcgcctt
    34861 ctaagcgttt gagcagaccg ggaatacgca ggtcggcagg gccaacgttg gccattgggt
    34921 tgattgcgcc gccgagcaca tggtataggc cgtggtattc gcgcgtgcgc tcgatgctca
    34981 tcacatcctt gggctcttcg accacgcaga tcaccgaacg gtcgcgtctc gggtcggcgc
    35041 ataccgggca ggggctggat tcgcacacgt tgccgcaaat atcacagaag cggatcttgg
    35101 ccttgacctc ttcgatggcc tcggccaaat cgcgcgcttc ctcttcgggc gcggacagca
    35161 tgtagaaggc gatacgctgc gcgcccttcg gcccgatgcc gggcagccgc ccgaaagcgt
    35221 cgatcagtcg ctgtattgcg ccgtcataag caagtgccat gggtattcct cgttattcgt
    35281 cctcattatt catccgagtg cttcttggca ggctggatgt tcttggggtt cttcggatca
    35341 tctgccgcga attgctcgac ttttttgacc tcgaatagct ttttcagatc gtcgagattc
    35401 atggccgtcg cctcgccgag cgactggtcg ctcatcgagt attcgtcatc ctcggccgcg
    35461 acttgcggtt gtggtttcgg ctggggctgg ctattccacg gatcggcatt cggggtcggc
    35521 tgtgcgggtt gcggttgatt ccacggatca ccgtgcgggg tggcctgcgg agccgattgt
    35581 ggaactgcct gcggttgatt ccacggatca tcagccggaa ccgctgatga tgaagactga
    35641 gcgggttgtg gctgattcca cgggtcatcg c
//
LOCUS       DAWBMW010000004        33107 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_402_length_33107_cov_3.353715, whole genome shotgun sequence.
ACCESSION   DAWBMW010000004 DAWBMW010000000
VERSION     DAWBMW010000004.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 33107)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 33107)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..33107
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_402_length_33107_cov_3.353715"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    17445..17454
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         6374 a        10016 c        10052 g         6655 t
ORIGIN      
        1 cctctgcatt cattccaccg cagagggtct tttcatttgt tttcgttgaa attctgccat
       61 ctcgcgtttc aaggggctga tttggggttt gccggaggtc tacttgcgtt tcgaggggct
      121 gatggtcaag tagatacgcc gccaaaatgg cggaatagcg ttgcgtcaaa gccgtttgtg
      181 tcggtgcttc accggatgag gagccccttg aaacggaagt agacctccgg aacatgtgaa
      241 atcagcccct gcaaagggag aatgcctcga tttcgccatg gattgtcgtg gattgctctg
      301 tggcatgggc aatactggga gtatgacaag cgcagatgag ttgaatgagc agtatgacgc
      361 tgacggtagc gtgacgcaag tgcctgaacg caatgatgaa gttcatgccg gcgaatccgc
      421 cgtcgcccaa tcccgcgaat cggccgtgac catccccgcc ggcaccgtgg tgctcgccgc
      481 caccccgatc ggcaatgtcg gcgacgcttc agcccgtctg gtggccctct tggaacgggc
      541 cgatatcgtg gccgccgaag acacgcgccg cctctacgat ctcgcccgtc gcctcggcgt
      601 ctatgtgaac ggtcgcgtca tcgcctatca cgaccacaac gagcgcgaca aggccgacgg
      661 cctgctcgac caggtcgaaa ccggcgccac cgtgctcgtc gtgtccgatg ccggcatgcc
      721 caccatcaac gaccccggct tggccatcgt gcgccgtgcc attgaacgcg gtctgcccgt
      781 cacctgcgcg cccggcccct ccgctgtgct cgacgcgctc gccctctccg gcctgcccac
      841 cgaccgcttc tgctacgaag gcttcctacc gcgaaagcat tccgagcgcg tgcagtatct
      901 gcgcacgctg ttgcccgaac gccgcaccat cgtgttctac gagacgcctc atcgcattgc
      961 cgattcgatg gacgatctgc tggacgcgtt cggcccgaat cgcccgatgg ccctgtgccg
     1021 cgaactcacc aaggattatg agcagattcg tcgcggcccg attgacgaga ttcgccaaag
     1081 tgtgattgat gatccgcccc gtggcgagat ggtgctggtc atcggcggcg cgtccaacga
     1141 ggaggccgaa gcggccgcgc ccagcacgtt gagcgtggat gacatggccg tgctctccat
     1201 cgaccgtgct ttggaggacg gcctgcgtat caaggacgcc atcgcgcaag tggtgcagga
     1261 gcatccactg gctgacggat cgttggccaa tcgcaagcag gtgtatgcgg ctgtgctgaa
     1321 aatcaaggga tagcgcagca gccggcgatt atctccccgt tacgcttcca tcgactcgaa
     1381 caatatttcc aacgcctgcc ggaccccggg cccaccacgc tgcgagacca attccagcaa
     1441 aggtgcgtcg gctttgggat tggcaaccag ccatttacgc agttcgggca cgtgatatgc
     1501 gatgtgccag agcacggcaa catccgtatc gggcgaacat gccaccgcgg gcgtaagtct
     1561 gatgggcggt tcaggcggcg cggtcatatc cgagattgcg cgcgatgcgg attcggatga
     1621 gtgtgccgcc gccgcgtggc gaggcaaccg ttgcgcctgc cgcaaatgcc gtaaacggtg
     1681 agcggcatgg cggcctctgc gatggcctct gacaatgaac cccctcgtca tgcgcattgc
     1741 tccaaagccg gttcgtccat ctcctgctgc accgattcaa aaaacatgcg tgcgcgtccg
     1801 gcgtacttgt gatgccgatg ctccttgacg atttgcagac agtcgtttgg ccgaaactgg
     1861 tacgtggaca tcagctgaca caccagtgcg ttgacggtgg aaggatcggg gatgtcttca
     1921 ggactcatca ccaggtcgca agccgtacgg gctggcgtgg tgacaggcag cccgccgagc
     1981 ttgatgattt gctccggcaa cgtcaatctt ttgaatacgc ggatacggcg gctgacggat
     2041 gccgagcgga agtgcgatgt ggaaatcacg tctatggtac tcggaaagtc ggggcctccc
     2101 agccataccc aagcggcggt catcgaacac accacagtgc caaacggggc gacctccgcg
     2161 acgattttcg cgcgaccata cagtgtgccc gaatgctcga tccagtagcc ggcggaatcg
     2221 tcgagcttac gtaacgtgcc gaaggcggcc agacggttaa ggctgagcgg tcccggcaga
     2281 tccttggtaa acagtatcgg cggcaccgca ggctgcgcgg ttgccggcga ggcgactgtt
     2341 gtgatgtccg tcatcggacc tgctgtgata tccatgaccg aacaatatgc agaaatgcgg
     2401 cttgcgcgcc accggatagg cgaaaacggt ggataagtgc ataactttac taggtgtgca
     2461 aatgatgtgg ataactcggt ggatattggc aggcggaagt gtgtgcggat gtgcgtgggc
     2521 gtgcgtacgg atgggtgcgc ggatacgtgt gcgaatgcgt atgtggataa gcgcgcaaga
     2581 ggtgtacgga taagtatgtg gggaagttgc gggtggatag gtgagtggga tgaggatggc
     2641 ggcgcggcag ctatccgggc gtgtccccgc cgcgccggat aatcaagcct tatgagtcat
     2701 gttttggtga atgttgcttg gccgtatgct aacggccccc gtcatatcgg ccacgtcgcg
     2761 ggatttggtg tgccctccga cgtctacgcg cgctacgagc gcatgaaggg caatgatgtg
     2821 ctcatggtgt ccggcaccga cgagcacggc accccgattc tcgtcgaggc ggagaaggaa
     2881 ggcctcactg cccaggaact ggccaaccgt tacaaccgcg tgatcgccaa ggatctgtgc
     2941 gacctcggcc tgagctacga cctgttcacc cgcaccacca ccggtaacca cgagcacgtg
     3001 gtgcaggaga tgttcaagca gtgcttggag aacggctaca tctacaaggg cacccagcag
     3061 gtggcgatta gcccgtccac cggccgcact ctgcccgacc gctacatcga gggcgagtgc
     3121 ccgatttgcc acgccgaagg tgcgcgtggc gaccagtgcg acgcctgcgg caacgagctc
     3181 gacccggacg agctcatcaa ccccgtctcc aagattaacg gcgaaacccc gcgcttcgag
     3241 cagaccgagc actacttcct cgacctgccg gcgctcgccg aagccaacaa ggcatggctg
     3301 gagacccgca agggctggcg taccaacgtc atcaacttct cgctcggcct gttcaaggag
     3361 gtcaagccgc gcgccatcac ccgtgacatc gactggggta tcccggtgcc ggtgaagggc
     3421 tggattgaca acccgaacaa gaagctgtac gtgtggttcg acgccgtgat cggctacctg
     3481 tcggcctcca tcgaatgggc ccgccgccag ggcgacccgg agaagtggcg cgaatggtgg
     3541 aacgacccga gctgcccggc ctactacttc atgggaaagg acaacatcac cttccactcc
     3601 cagatctggc cgtccgaaat gctggcgtac aacggcaagg gctccaaggg cggcgaaacc
     3661 ggcccgatgg gtccgctcaa cctgcctgag caggtggtgg ccagcgagtt catgaccatg
     3721 gaaggcaaga agttcagctc ctcccgcggc atcgtgatct acgtcaagga catcctcgcc
     3781 cgctaccccg tggacgccgt gcgctactac atttccgtgg ccggccccga atcctcggat
     3841 tccgacttca cgtgggccga gttcgtgcgc cacaacaacg aggagctcgc ctcctcgtgg
     3901 ggcaacctgg tgaaccgcgt ggccaacctc atcaacaaga acttcggcga gattccgccg
     3961 ctggacgagg attcgatgac caacgaagac cgtggccttc tggaagaggc ttccgccgcg
     4021 ttcgacgtgg tcggctcctc catcgaaacc caccaccaga agcacgccct gtccgaggcg
     4081 atgcgtgtgg tcggcgacat caacaagtac atttccgcca ccgaaccgtg gaagattaag
     4141 gacgaccagg ctcgtctcgg caccgtgctg cacgtggccg ctcaggccgt gtctgacgcg
     4201 aaccacctgc tcgcgccgtt cctgccgcac tccgcacaga aggtgtggga ggcgctcggc
     4261 ggcaccggca ccttctcgcc gctgcctgag ctcaaggaag tcgaggatct cgacaagccc
     4321 ggcttcacgt acccgatcat caccggcgat tacgagctcg gtgtgaacgt gcacccgtgg
     4381 aagagcgagg caatcgaggt cggcgccatg gtgcctaagc ccgcaccgat cttcgccaag
     4441 attccgaccg aggccgtgga agaggagctg gctcgcttcg atgaagcgtt ggctgctcgc
     4501 cgtgccgccg aagccgaacg tctggccgcc gagaaggcca agctcgccgc cgagtgattc
     4561 ggcattgcta gctcgtagct gacgggccac ctcgcacatg aagtgttgcg gggtggcccg
     4621 ttgccgtatg tgcggcctag ccccgacaat gcgaggccgg ttgtcgggcg gcgttacaaa
     4681 cccgtcacag tttgccaaga cttttctcag acaagcacat cgaatcccca agaaagagac
     4741 ggttatatag aagatgtcaa cggaatgaag ttcataccgg tggcgaggtt cttccgaacc
     4801 ccataactga acccttcttc tctcttctct ctcacccggc ggccccctcc gccgggtttc
     4861 ttcgttttgg gccgcatcat gcaacgcaag ttacgctgct gtcggagcat tgcaggattc
     4921 cctccatgct tccggtttgg ggggtgctgc ttgattcctc tacttccagc gaaacgttgc
     4981 tatcgcggta tggtcgcatg cctgttaagc attgaagcat cttatctatg cgcagtcggt
     5041 gtcccggtgg tgacggcaac cgattgacgt gcgaaccggg ccagatttcg ttgtcccggt
     5101 tcgtgatttt ggcctctttt ggggaaccgg gacagaccat cccgtgagcg ctcaatgtat
     5161 gcaaatccag aagcccggaa ttccgccgat gtgtgatggc ggatattgtg cggtagggcc
     5221 gaattggcga tttttcgagg gtgtttctgt cccggttgct caaaaagagg cgaaatggag
     5281 aaccgggaca acaaaatctg gcccggtaca cacataaatc gattgacgtc gttaccagga
     5341 catcggacac cgtgacgtat atcgcgaacc gcataatatg gcagtacgca acgcagatga
     5401 tgtgcggatt cgccggacgt aacatggcgt gtgacacaca acagtgccat gcggtcgggg
     5461 ccacgcgacc aaatggggtg cgatgaatgg catatgcgtc gcagcaagcg cgcgcaatcg
     5521 tatcgatgtc agctcagtgc gccccggtgg atttccgtcg tttccgccgg gcaagttcat
     5581 tgagcggcgt cgctgtcaag gtgaaatcga atatggcgcc tcgcaacgcc agctgtcgcc
     5641 cggctcggta ggcgagttcg gcttgagacg gttcatcagc catattcgcg gcggtggcga
     5701 tgaggacgat ccaatgatga tgccgtaaca attcgcgctt gtcgttgtct cgccgccact
     5761 gggccttgtc tgtgcgatga tgatcgccgt catattcgat ggcgacgcgg aattcgggcc
     5821 aagcgagatc gagtgttatc ggcgcaccat tgctgaacgt cgcgccgggc actgtgtaat
     5881 ttgtcacggc tcgtggaagc ccgtattgcg tggttgtgat acgggtctct gattccatcg
     5941 gagaatccac gcgcggtgca ataaattcaa gtgcgatcat gcatgtggct ttcgcgttga
     6001 atttcggaag ttcgctgacc tgccgcacaa agtcctgata aatttcgtcg gcggtgcggc
     6061 cgcccgatag gctcggcttg cgcgctattg ccgtaagggt tgcgtcgcca agtatgacca
     6121 atgattccaa tgacatgtga gcggcgagct gcgcccaagt atgtaccaga ttcagcgcgt
     6181 gcacatgctt gttgatacgt gcattgtgcg cggcgctcag cggtttccag atgtgcggtg
     6241 ttgttggcgg aacgacggcg cgtatccggg tgcgatgcga gccggaaacg gtatgaagct
     6301 cggtggcatt gagatcgcaa tccttgggga gtggcacgga ttgcaatatc agcgatgtgg
     6361 tcatgccgaa gagcaccggc tgcccgagct gttgcgcggc gtccgtgcat cgtttaagca
     6421 tttcccgctt acgttgggtg agggtttcgc tcattgcagg cgcagtggaa ctcgccatac
     6481 gcggtggtga gtatggctcg gggttgatgg cgtgcgtggg ctcggcactc ggtgccgcgg
     6541 cattgtgggt ccagccgggt ggttcatcgt cgaatacgcc gggtggtggg gtgggaaatc
     6601 cgctgttcat aacgccaccg tagcgtagcg gtcgcgtatt cgccatgaaa aacggggtat
     6661 gtggttctag cttgctgagg atgtggataa ctctgtggat aactgaagtg ctcctatgtt
     6721 ttgctcactc tgcgaggagt cgacaaaaca taggagcact tcagccgcgg ccaatgggcg
     6781 gaatatgtca gcggcagcga cgggtgagag gctgccaggg atcgagctcg actggttcgg
     6841 tggtgagcag ggcgcgggtg gcgtcgtcga ggtattcctt gcggatgatg agctcggtaa
     6901 cgtagcggtc gaaccaggcg tcggacatga cgtagtagcc gtccttgccg gcgtccttgc
     6961 cccagctgtt ctccaccttc cagcggttcg gcttgccgtc ctcgtcgagg ttcacgccgg
     7021 tgaaggtcat agcgtggttg ctcgggctgt cgccgtattc gaggccgtgg gccttgtcgc
     7081 cggtgaattc ggtgccgaac agctggtcga cgcgcaccac ggactggtcg aagaaaccgt
     7141 ccttgcgcag ggcgaactgc gtgcagtcgc aggcgaacca gatcgggtgg ccggcggtga
     7201 gctggtcaag cgcggccttc ttgaacacgt ccagcggcac gttgacgaat tccatatcgc
     7261 cagcctcggc cacgttcgcg ctgaagcgga tgcggtagcg ctggttgaac gggcggttct
     7321 tgagcggggc gttggccagc gtcacgaaat cgttgacgtc caccggcacg tacttcttgt
     7381 agaactcaag cggggtgatg ccgatctcgc gaatctgcgg gcgctcgtcc ttgccggttt
     7441 tcggcttgtc ggacttgtcc ttgtcgcact tgcaggcctt ggcgtcggat ttgccctcgc
     7501 acttgcagga ttcgccgcac ttgcaggatt cggccttgcc gtctgcgtcg gcctcggcct
     7561 tgcacttgcg agcctcgccc ttcttatcct catcatcatc cttggggcgc gcgaagaagt
     7621 cgaacttctc cggcggttcg ccgagcgcca cggcgcagat gcggtacacg gtgcccatgt
     7681 actcgtcctt caacgcacgc agctcgtcct cagacgcccc ggcagccgaa cggcggcgca
     7741 gctcggccgc gaactcgcgc agcttggagt tgaggtactg cttgaaatcg tcggaattac
     7801 gggagttctc agactccggg taggcgctct tgggcactag gccgtacttg ttcaccagtg
     7861 cagcgaacat ctgccaccag ccaccatcgt ctgcaggcga ctcattgatg gcctcgaaca
     7921 gacgcgaatc ggtcgcctcg tcgagcgtgc gcagcacgtt ctccagataa gtgttcgact
     7981 tctccatcgc gtcccagaag aacaggtagg tctcggagaa ctcgaaatcc tcaagattcc
     8041 agcgatgcat gagttcgtaa cgcagcgtgt tcagggaggc gaacatccag caacggccgg
     8101 aacgctcctg attggtgatc gaaccctgct tgagttcgat ggagaagtcg cgcggcagcg
     8161 cacgtacgcc gcgatagtcg gtggccgcct tgagcacgcc ggccgaaacc gccgcgtccg
     8221 ccgcgatcag gttggcgcgg tctttgttga agttttcggt gtacgtacgc agaagttcgg
     8281 gtttgatggc ccgtgcgctg gcgtttgctg tcatgatgat tgatcttcca ttcatgtagg
     8341 cgacgtttgt ggtccgtcgg tagtataagt cgccgtgtat tcgtcgggcg attcaccggg
     8401 cgaaatcgta cgggcggata tgcgccggcg attccacccc gccaacgatt tgtccacctt
     8461 gctgctcgcg cgggcgcacg tatcatatgt accgatacat attgttgacg aatgtcgcat
     8521 agtccaaagg agtacgtcat gacttcgctg catagcaagc cgctggccct gaaaaacgtc
     8581 accgtcaccg attcattctg gcgcaccgag caggaactcg tgcgcacggc tgtgattccc
     8641 tatcagtgga acgccctgaa cgataacgtc cccggcgcgg cccccagcta ttgcatgcac
     8701 aacttcaagg ccgctgccgc gcaaaacaag cgcaaggata cccagggcaa cgcgttcgtg
     8761 ccgccgaagt acacgttccg cggatttgag gcgctccccg aagacccggc caatcccgac
     8821 ccagacaagt tctacggctt cgtgttccaa gacaccgact tctccaagtg ggtggaggca
     8881 gtcggctatt ccctcgccca tcacccggac cccgcactgg aacagaccgc cgaccaagct
     8941 gtcgacatcg tgtgtgccgc ccagctcgac aacggctatc tggacgccta ctacatcctg
     9001 aacggcatgg atcgcgcgtt caccaatctg cgtgaccatc acgaactgca ctgcctcggc
     9061 cacctcgttg aaggtgcggt ggcctactac cagggcaccg gcaaagacaa gttgctcaag
     9121 gccgcctgcc gtttcgccga ttacgtagac gagcgcttcg gtcgcaagcc gggccagctg
     9181 cgtggttacc ccggtcacga gattgccgag atggctttgg tgaggctgta cgaggtcacc
     9241 ggcgaacagc ggtaccttga tctggccgaa tatttcgtga ccgagcgagg ccggcagccg
     9301 tacatcttcg atattcaggc agacgagaac gcaaagcgcg acgccgacgc caactacaaa
     9361 cccaacacgg accccaaccg gtacgcctac caccaggcga acaagccggc caccgaacaa
     9421 gacgaagccg taggccatgc cgtgcgcgcc ggctacttct actccggtct ggcggacgtg
     9481 gcccgactga ccgacgatca ggacctcgcc gatgcggccg agcggctctg gcgcaatatc
     9541 gtcgataaga aactgtacgt taccggcggc atcggcggca cggtggacgg cgaggcattc
     9601 tcctacaact acgacctgcc caacgactcc gcctactccg agacctgcgc cgcgatttcg
     9661 ctggcgttct tcgcccgtcg catgctcgag ctcgcgccaa aggccgagta cgcggacgtt
     9721 atggagtcgg cgctgtacaa caccacgctg gccggcatgg cgctggacgg caagagcttc
     9781 ttctacgtga acccgctgga agtcaatccg tatgcctgcc ataaagacag ccgcctgcgc
     9841 cacgtcaagc cggtgcgtca gaagtggttc ggctgcgcat gctgcccgcc gaacatcgcc
     9901 cgcatcgttg agtcggtgca ggaatatgcc tacacggttg ccgaggatgg cggtacgctg
     9961 ttcacgcact tgtatatggg cggcgtggcc aaagctgagt tgaatggtac ggctgtggag
    10021 ctggatgtta cggcgaacct gccgtggcaa ggcgatggca aggcggtcgt gcgcttgggc
    10081 ggtgatgctg ctggcacctc ggctcaggct cctgctcgct ttacgctggc cttccgtctg
    10141 cccggttggg tgggcgatga gtccgctgct gccgccgcga ttacggccac gggcgagtct
    10201 gagtctgggg ccgatagttc ccgcgtgacc cgcgaaattc gcgacggcta cctgtatctg
    10261 accggtgagt ggcgcgacgg cgacaccgtg acgttcgact tcccgatgcc ggtgcgtatg
    10321 ctggccgcca acccgctggt gcgcgaggac gccggcaagg tggcgttcgt gcgcggcccg
    10381 atcaccttct gcgccgagga aaaagacaac ggcgccaacc tgcacctgct gcacgcggat
    10441 gtggaggcac tgctggccga cccgagcgcg gccaaggtcg aggagttcga cttccacgcc
    10501 ggcgccaagg gcatcgacga caagggccag ggcgaggtgg aagacgtgac ccgccgcatg
    10561 gtgaagcttg aggtgccggc ttggcgcgag ccgttgccgg ctgctgtggc cgccgcgggt
    10621 gagggcgcgg ccgaggttcc ggcgttcgcg ccgctctacg ccgtgtacgc gccggtcaag
    10681 cgcgagcccg ccaccgccac gctcatcccg tacttcgcct gggccaaccg cggcgaaaac
    10741 gaaatgacct tctggctgcg cggctgatat ttgctctcgg ctactctggt agccatgggc
    10801 atcacgacat gtcggctcga agggccggtg gacttttact cggtcgggca gtttgtgtcc
    10861 ggaccgggct ggcgccatct gcggcgcacc atcgacacct tcgagctgat gttcgtgcgt
    10921 cggggcgtcc tgccgatgcg ggtgggggag cagaccctgc atatcgaggc cggccaaatc
    10981 gcactcttgc cgccgaatgt ggagcatgcg ggcgccgaca tcatcacgtc tgacgtggac
    11041 ttctactgga tgcatttcaa attgccggat gcgcgcatgc tccccgatga cgcgggtctg
    11101 ccgcaggacg accattgcct gttgctgccc gatgaacgca ccctgcccga ccccgaccgg
    11161 ctggcggtga tgtgtgggca gctcatcgac atctatgcgc gattcggccc gtattccaac
    11221 gcttactgcg attacttcgc caccggcctg ctgctggagg tcagcgcgca ggagcggctg
    11281 aaggcggact ttgccgccca tcgtacgctt gccggctacc ggggcgacgg caagcccatc
    11341 accagtgccg accgcgtccg gcagcaacag gaatgggcca tgcatgtcga ttcggccggt
    11401 gccgctggcg attcgtccgg cctggcgccg atgctggcca tccgctcgtg gattatggcc
    11461 aatgccttcg acgacatcac cgtggccaaa gtggccgcgc gcttccacta ttcacccagc
    11521 tatctgaccg cgatgtaccg gcgcgtgttc ggcgtgggtg tggccgagca gatcatcgaa
    11581 tatcgcatcg accgtgcgcg cgaactgctg agttctaccg cttccagcgt ggcggatatt
    11641 gcgcgagaag tgggctacgc cgatcccaag tacttcatgc gcgtcttcaa gcgccgcact
    11701 ggcctgaccc ccggccaata ccgcgacgct ttccccgccc gcctctacaa caccgtgtga
    11761 ttagggaagg tcatggtcct aaccctagga aaagtgcgca tgggcgccac taccacgtgg
    11821 gagcgctggg aacggtttga cgcatgccga aacctcgcac gtcacgctgc acggcacttg
    11881 ggcagtgggc gcggcagtcg ggaatcgccg ccctatttcg gcgtttcagc gttacctgtt
    11941 tgccgaaccg acagtggact cgcgcggaac cagtgtgtat ccaatggtgg cgcgcgtggc
    12001 cggcatgcca gcggattcgt cggacctgcc gtcgccctgg tcgcgctgct ccacgcggcg
    12061 ggtgatcata tcaagcgcga attgggcgag ctgatcgaga tccacttgga cggtgctcag
    12121 cgtcggtgtg gtgtacgagc cggccgtggc gccgtcgaag ccgatgactc tcacatcgtc
    12181 gggcacgcgg atgccgtagt cggcaaggcc tcggattact ccgaatgcgg catagtcgtt
    12241 aatgcagaag atgccatcga tggccggggt gcttcccgtt gcccgcgtgg tgcccgggga
    12301 acgggactca gcagtgtcat cagccgattg cgcacgagcc tcgagcatcg cctcggcgat
    12361 ggcatgaccg gcttcgatgc cgctgtcgct accgtaacca atgaagttcg tcgattcgtc
    12421 atacggcagc ccggcgtcaa gcagtgcgcc gctggccccg cgcaggcgca aaccgctgga
    12481 actttccacg tgcgccagtt cggttcgtgg cgaatacccg tcgccgacga tggcgatgcg
    12541 gtggcatccg cattcgatga gatgctgcac tgccgcccgc gcgccgtcct cgttcgggaa
    12601 attcactgta tcgaacgtgg gcgtttcctc gcaggcgtca atcagaacca gcggtcggcc
    12661 gtggttgatc gcggaaagcg gcacaccggc attcaggccc gaagcgtgga taatctcgcc
    12721 gtcgaacaac tgcccggaga acgggttgtt ggccagcgcg tgtttggcgg catccggaga
    12781 gtaccgggtt tgctccacga acggcgtcag cccgcgctcg gtggtttcgg tgacgagcga
    12841 ttcgacgagt ttggagtaga acggcgaatc gaattcgttg acgatgatgt ggatgatgtt
    12901 gctgcggccg gacttcagca tgctggccgt cagattgatg cggtaatcga gcttttcggc
    12961 cactgccagc actttggcgc gagtggaatc cttaacaccc ggcttgccgt gcagcgcatt
    13021 cgaggcggtc atgggggaga ccccggcggc tttcgctacg tcgcgcaatg ttggtttgcc
    13081 gctcggcata acgtcacctc atgtttcgcc tgtgatgggc attggatcat gcccgtaatg
    13141 cttccaatgg ttgtaacggt attcgcaaca tcgtgttttg gcaaatggtg gctgtgtgcc
    13201 ggaatcgctg aaaatgctgc gcttaggcga tgacgcgccg agcgtaacat gcgcttgctt
    13261 gatatcgttc cggtggaaga atatgtaacg gtacatacgt cggtgtgcca agaggccatg
    13321 aagttcgtcg tacgttggga tgggactctc cgggggagtg gcgtcactct tcacctatcc
    13381 gtacgtgcgc gatgccgatt ggtttgcggc tggcgtaccg ccgtatggca acttatacga
    13441 atatcaacga aagcaggata cgatgacgga tcttccgcag agccttcctc aggcatggcg
    13501 tccgccgatg ggctggaaca gctgggattc ctatggcacg accctcaccg aagacgagct
    13561 gctggccaac gcgcggttca tggctgaaca cctgaaaaac gccggctggg atacgctggt
    13621 catcgacatc gactggtacg acccgaccgc ccgcgcacat ggttacaacg acaacgcgcc
    13681 gctgattctc gacgaatacg gccgtcagct gcctgacccg gtgcgtttcc cgagtgctgc
    13741 cggcggcaag ggattcggcc cgctcgccgc cgccgtccac gaactgggct tgaagcttgg
    13801 catgcacatg atgcgcggca tcccgcgtat cgccgtggac aagaacctgc ccgtatacgg
    13861 caccaactac accgccaagg acgtggccga cctcgaccac gtgtgcaagt ggaatccgga
    13921 caactacggc ctgaaccaat cccacccggg cgcgcaggcc tggtatgacg cgcagctcga
    13981 cctgttcgcc tcctggggcc tcgacttcct caaggtcgac gacatgcaga ccccgttcca
    14041 ctccgacgaa atcgccgcct atcatcgcgc catcgccaaa gccgaagcca aatacggccg
    14101 ttcgattgac ctgtctctct cgcccggcgg ctgggtggcg acgagctacg tcgacttcct
    14161 gcgcgagaac gcccaaatgt ggcgtatttc cgacgatctg tgggaccgtt gggaagacat
    14221 ctaccagcag ttcgcccgcc tggcccgctg ggcgccgttc cagaccaccg gccactgggc
    14281 cgacgccgac atggtgccgt tcgggcacat aggcctgcgc gccgaacgcg gcgacgaccg
    14341 ccaatcccgc cttacgctgg acgagcagaa gacactgctg gccctatggt gcatggggcg
    14401 tagcccgctt atggtcggcg gcgatctgcc caccagcaac agcgacgcca tcgcgctgct
    14461 gcagaatccc gcgttgcgtg aggtgcttgc cggctccacc aataaccgcg agaccgtgcg
    14521 cgaacggatt tttggcaaat ggtgggatga gagcacctac cgtggcgagt tcatcgtctg
    14581 gtccgccgac gcagccgatt gggctgatgg cacacgttcc gcgcatcacg gcggccatta
    14641 cgccgcgctg ttctggaccg gcagcgacac atacgagatc ggccgcaaca tccagctcca
    14701 atccattgtg gggcttgacg cccgtaatga cgattggaca ctcgccgatc tgtatgccga
    14761 cgcgccgggc gagcccgccg acgtgcgtct tgaaggcgtg ggtgccgacc gcgtcatcac
    14821 cggcacgatt ccgccgcacg gcgtgctctg ggtggccctg gaccgacgct gaaaccgaac
    14881 ctgcctccgc caaccaacct cctgaaccga ccgcccgaat cgtatggaga aacctgccat
    14941 gaatgcgaac atcgcaaacg aacaatcccc ggtagctgtt ccgcccgcaa gttggttcct
    15001 acgtgcgttc gctggcgttc tgacctgtag tctgtctgtc tctgtctgtt cgttgcttcg
    15061 ccttgcttgc gttttgtacg ttttatctgt ttcgtgagtt tcgtgcgttc tgcggattcc
    15121 acgcgtttcg cgcagtgccg cacgctgatc accattcagc gtgcggcact tttgcatatc
    15181 tcacgtgttg cgggagatgc cgattccgtc gcacaatacg taaaacgtga atgaaccact
    15241 cactatgctg ggttgtttga gcgtgatatg acatgcgcca ctttcccggg aatgcgttgc
    15301 gaattggacc tgttcggagg tgtgcagcga cgcattccgg ggaatgttgc gcttagagag
    15361 ccgcgtatcg ttgctaatgc ggggaacacg cagagttgcg ggccgttgcg attagacgca
    15421 ggtcgcctag actagtacgc gtttgcgaaa gtcgaacgtg cttcgaccga tgaaggagag
    15481 cttcggtcat gcggccaatg acaggcgcag tgcgaagcac ggctgatcgt gaatcgcggc
    15541 ggcagcgatt gccgcgtgcg gcgcaggagc atgccgcacc ataccacaag caaacaatcc
    15601 ccaagaaacg gtgatctcct cacatgtacg tcgatccggg aatcaatacc agcgcaagca
    15661 atctcaaatg ggtgctgcca tactgcaaac cagacctgcc ccgcgtggta ggcgcgcttc
    15721 tactgttcat cgcaaataac acgatggccc tgaccattcc gattctgtcc ggtatcatcg
    15781 tggaccgcgt catcgtcggc ggacatgccg acgaactgcc acgtctgtgt ggactgatga
    15841 tcctcatgac catcatccgc gtgggcgcgc gatacggcta ccagatgtgg atggagcgtt
    15901 tcggccagaa ctcggtctac cgcctcgtct ccgacgaata cgagaagctg cacgagctcg
    15961 acttcacgta tttcaaccac acacgcaccg gcgacatcat gagccgcctg acctccgaca
    16021 ccgacgcgat ccgccactgc ctgagttggg tgagctatca gattgccgac tgcgtggtca
    16081 tgttcgtggg cgccctgtgc gtgatgttcg ccatcgattg gcgactcgcg ctagtcctgg
    16141 cctgcgtcac gccgttcatt tttgtgctca cgcgcggcct gtccacgcac gcccacccgc
    16201 tgttcttcgc catccgcaac tcgctggcct ccctcaactc catggtcgaa gagaacatcg
    16261 agggcaaccg tgtggtcaag gcattcgtgc gcgaaccgta cgagaccgaa aagttcgacg
    16321 agcacaacga cgattacatg cagtgcaaca tggccttggc ctacaactcg cgcaagtaca
    16381 tgccgtggct ggacggcctc ggcttctcgc tgcagctcat caccctcggc ctcggcggct
    16441 ttctggtcat caagggctac atgaccctcg gcaatctggt gagtttcaac tccttcctgt
    16501 ggatgatcga cggcccggtg cgccagtccg gctggctgat caacgactgg cagcgattca
    16561 acgccagctg catcaaaatc cgcaagctgc tcaccgaaca gccgcgcatc gtggagaagc
    16621 ccgacgcggt cgaagccgtc aagcaggcgg tcacgatcgt cgagcaagtc aagcacgatg
    16681 atgaggctac cggggctacc ggagcgcccg tggcttccgg aacatccggt gcccagcagt
    16741 ccaccgaacg caagtcctgc ccgctgcaca tcaagggcga catccgcttc gaccacgtca
    16801 gcttcgcctt cccggacgac ccggagaccc cgatcctcaa ggacctcgac ttcaccgtgc
    16861 ccgccggctc caagctcggc atcctcggcg aaaccggcgc gggcaagtcc acgctcgtca
    16921 gcctcatctc ccgcttctac gacccgaccg ttggccatgt gctcatcgac ggcatcgacg
    16981 cacgcgactg gccgctggcc accttgcgct cgcaggtgtg catcgtggcg caggatacct
    17041 tcctgttctc cgacaccatc ggcggcaaca tcggttttgg tgccagcggt gatagcgacg
    17101 accgatatat ccagaaaatg gcccagatcg ccggcgcgga caatttcatc aggtcgatgc
    17161 cacagggtta tgacaccgtc gtgggcgaac gcggcgtggg cctgtccggc gggcagaagc
    17221 agcgcctgtc gctggcccgc gcgctcgcgg acaatccgtc gatcctgatt atggacgata
    17281 cgacctcggc cgtcgatatg gagaccgagg cggagatcca gaagcacctc aaggagatgg
    17341 acggcggcaa gaccatcgtc gccatcgccc accgcatctc gtcggtcaag gattccgacc
    17401 tgattctggt gctcgagcac ggacgtatcg tcgaacgcgg caccnnnnnn nnnncgccga
    17461 actcgtggcc gcacacggcc gctactggga catctaccac aagcagctcg gactccagtc
    17521 cggcgtcgca caaggttttt agaagaggac ttatggcaca acgcaataca taccgcgagg
    17581 atgaggaact cgaggagcag atcaacttcc gggacatcct gcgcgtcggc aaatatctca
    17641 aaccgtatat ggcacaggtg gcgcgcattc tcgtcgtcgt catctcgatg agctgcatcg
    17701 tcgtcgccgt gccgtacctg accaagatca tgatcgacga ggcgatcccg aacaaggacc
    17761 tcggcaagct cggcatcctc gccgtgatcc tcgccgcgct gatcgtgctg tacgagttcg
    17821 ggctgcgcta ccgcacggtg gcgatcacgc gcgtcggcca gctcatgctc aaggacatgc
    17881 gccgcgatat cttcacgcat atccagacgc tgcccttcag ctacttcgat tcgcgcccgc
    17941 acggcaagat cctgatccgc gtggtcaact atgtgaacac gctttcggac acgctgagct
    18001 ccggtctgat caacgtgatc tccgacgtgt tcaccttcgt gatcacgctg atcgtgatgt
    18061 tcgtgatcga ctggcagctg gccctgtgga gcctgatcct gttcccggtg ctcatcatct
    18121 gggtgcgcgt gctgcagatc ttccagcggc gcgccttcca gaagctctcc aacaagcaga
    18181 gcaacctgaa cgcctacatc cacgagtcga tcgccggcgt gaagaccacg cagaccttcg
    18241 cacaggagca ggcccagttc aggaccttcc aggaacagca gggcgaggtg cgttccgcgt
    18301 ggatgcacgc cgtgcacatc cagttcctca tgtggccggg cgtgcagacc atctccgtga
    18361 tgaccatctc gttcatctac ttcgtcggcg tgatgggctt cgggggcgtg aacgtgacca
    18421 ccggcgtgct catcgcgttc gtcggctacg cgaacaactt ctggaatccg gtgatcaaca
    18481 tcggcaactt ctacaaccag ctcatcacct gctcggcgta cctcgagcgc atcttcgaga
    18541 cgctcgacgt cacgccggag atccgcaaca agcccgacgc ggcggacctg ccgcagatcc
    18601 gcgggcgcgt cgacttcaac gacgtggtgt tccgctacga gaaggacggc cgcaacatcc
    18661 tcaacctcgt ggacttccac gtcgaaccgg ggcgcacaat cgcgctggtc ggcccgacgg
    18721 gcgccggcaa gacgacgatc atcaacctgc tgtcgcgctt ctacgacgtg tccgagggct
    18781 cggtgacgat cgacggctac gacgtgcgcg acgtgacgct cgcatcgctg cgccggcaga
    18841 tgggcgtgat gctgcaggac accttcatct tctccggcaa cgtgcgcgag aacatccgct
    18901 acggcaagct cgacgccacc gacgaggaaa tcgaggcggc ggcgaaggcc gtgcacgcgc
    18961 acgagttcat catggaactg cccgacgggt acgacacggt ggtggaggag cgcggatcga
    19021 ccctgtcggc cggccagcgc cagctcatcg ccttcgcgcg cgtgctgctc gccgacccgc
    19081 gtatcctgat cctggacgag gcgacgagca acatcgacac gcgcaccgag gaggcgctgc
    19141 aggccggcct gcagcatctg ctcaaggggc gcacgagctt catcatcgcg caccgcctgt
    19201 cgacgatcga gaacgcggac gagatcttct acatcgatca cggccagatc gtcgagcacg
    19261 gctcgcacgc gcagctgctc gccgccaagg gcgcgtacta ccgcctctac gagtcccagt
    19321 acgcgatgat tcgcgtggcc agtggagcgc cggaagagtg acgagatgtt gcgcacaagg
    19381 atgcgtttag cggcgtttca catagcgggt gctgcgaccg gcaccgattt tgatgatcgt
    19441 accggagtcc aacagtgatt tcagcgtgcg ctcaatggtg gtggtgctgg tatcgggcat
    19501 acgagcgaga atatcggcct tggagagtgg cattagggat cgatcaagca gtgcttcaat
    19561 ccgcgttccc ttggtggcta ctcgcggtgc tccctgcgtt ctgccgcctg ccgtgagatt
    19621 gatgcgttcg tcgaattcct tgcacgcgga cagtatgatg ccaagcaggt agttcgtgaa
    19681 tggcgcataa tcattggcgt tgtcgtccca tccttgggtg cttgcggcca gcgcctcata
    19741 ataagtgctt ttactttgct cgataaggtg ttcgatactg atgtatttgc cgaccatata
    19801 gccattgcgg tacaacatca ggagcgtgag tagccgactc atgcggccgt tgccgtcatt
    19861 gaacggatgt atgcaggtga aatcgagaat gaacatggcg atggcaagca atggatcggt
    19921 atgtccatct cggattgctt gcgtgtaggc ggaacacgcg cgttcgatca gttccggtgt
    19981 gacaagtgcg gacggtgggg cgaatcgcac ggtgctggtc ccgtgtgcat ctcgttcgat
    20041 gatgacgttg tcactgtcct tgaaatgccc cgcaacggtc agtggtgtgt gccggaagag
    20101 gtcgcggtgc agttgaagaa tgacgttcgg ggtaatcgga atatagtcgt ggctttcgtg
    20161 aatagccgat agcacgtcac ggtagccggc aatctcttct tcattgcgat tgcgcggtgt
    20221 gactttctcc gagacgagat cgttgagcct gctatcgctg gtagaaatac cttcgatgcg
    20281 gtttgaggca tctgtgcttt ggatgcgcgc gacggcaacc agcgtctcca atatatccgg
    20341 tctgatcgtt gtctgtatgc tttggcgccc ttttgcctcg tgaatcgcgg caagcaaact
    20401 cgtggtgtgc ggggtgagca acgtcttacc tgattctatg tagtcgaatg atctcatact
    20461 gcctcatttt ctgcatcata cgaacttttc tgcatcatat tttatcaaaa tgaggcagaa
    20521 aatgggctga tgatgcagaa aaaggcggca ggatggcgac cgtctcgctt gccggttgcg
    20581 ctactctccc gggaatgttg cgctcgtggc aagaaacggc aagttcaggc gggcgtgacc
    20641 gacgataccc gtcgtgtagt cgaacgtctt accggactgc cttacgagcg ttgctggcgg
    20701 tagcctacac tgagaaacag cctacactga gaaaccatga gcaagcacca ccatcgcgac
    20761 cgtagctggg ctccggcacc tgaagcattg ccggacgatg cccagaccat cgacaaccac
    20821 actcacgtgg cctccgtaat ccctttcgcg cgcgctatga gccatgaggc gcaggagaag
    20881 ggacagcccg aagtcccggt gtacgacgtc gaccaattgc tggcgcaggc gcaatctgtc
    20941 ggcattgggg gtattatcga ctgcggttgc gagctgccgc acctgatgac cgccgtccag
    21001 atggccctcg atcatccggg taatgtgcac gcggcgctcg caatacaccc caatgagtcc
    21061 gtgctccacg ggcatcgcgg tgtgccgggg ccggatggtc tgcccctcaa atacaagcct
    21121 taccatgaca cgtctttcga ggacgcgctc gccgaagtac atcgcctcgc caccacatac
    21181 cccgagcaag tggtggctat cggcgagacc ggcatggacc tcttccgcac cggcgaaggt
    21241 gccaaggaac tgcagcgcga ggctttccgc gcgcatattg ccctggccaa ggaactgggc
    21301 ctgcccatgc agattcacga ccgcgactcc catcgcgagg tcatcgaaac cctgctggcc
    21361 gacggcgcgc ccgaacgcac cgtattccac tcgtattccg gcgaccgtga gatggccgag
    21421 atcgcgcgcg aacatggctg gtatctgagc ttctccggca ccgtgagcta caaaggcaat
    21481 gacggtatcc gcgaatcgct acggattgtg gggctggacc atgcgatggt cgaaaccgac
    21541 gcgccgtatc tgacgcccat gccgtatcgt ggccgcacca acgcgccgta catgattccc
    21601 tacaccttgc gggccatggc cgatacgctc gacttgccca tcgcggatgt ggcgcgcggc
    21661 acccgcgata ccgcccgcgc cgtatacgga ctgtgagggc cgggtgtgcg gccgtcgcgg
    21721 cgatgtgttg acttgtaaaa tttgtcagaa ttgtgaacga ggtctaccat gatgtacctg
    21781 acataagcga gggggatgat ctccacctca atggaggact gaacgtatgg ctgagtcgaa
    21841 ccaaaggacc gcacgtgccc aaaaagcctc aaaggcccag aaagcccagt ctggtgccgc
    21901 caaagccaag gcccgacagg aagaagaagc actgctgagg gccgacacct ttaccaatgc
    21961 tcccaaggtg tcccgccgca ctctcaccaa ggaggagcgc gaggcactcg ccaagcagag
    22021 ggaagctgaa accaaagaag ccgagaaaat cgccagcgca tggtcgaaag gcggcctcgg
    22081 tcgatggtgg gcccgcctgc aatccagccc agacaaggtc acctggggca tggcaatcgt
    22141 ggcgctcggc gtggtgtacg gcgacatcgg cacctcgccc ttgtacaccg cgcagacctt
    22201 ccttgccggt cagggagggc ttggcagcgt agaccgagag gccgtgctcg gcatgctgtc
    22261 gctggtcttc tggtcgatta cgctgatcac taccgtcaaa tatgtgctca tcgccatgcg
    22321 catcgacaac aatggcgaag gcggcatctt cgcactgtac tcgttgattc gcaagtacgg
    22381 cgcatggctc gcgattcccg ccatgctcgg cggcgcggca ttcctcgcgg attcggtact
    22441 taccccggcg gtttccatca gttccgcagt cgaaggcttg cagaccctgc cgccattgga
    22501 agggttgttc gacgaaaacc caagcctgac cctgatgatc accgtcgtga tcatcgtgat
    22561 tctcttctcc gtgcagtcgc gcggcaccga aagcatcggc aaagtattcg gctcgatggt
    22621 gctggtctgg ttcggcttcc tcgccatcgt gggcgtcacg aatctctcta acgactggtc
    22681 tgtgtttgag gccctgaacc cggtgtacgg catcaaattc ctgttcagcc cgcacaatgc
    22741 tgccggcatc gcccttatgg gcaccgtgtt cctctcgacc accggtgccg aggcgctgta
    22801 ctccgacatg ggccacgtgg ggcgcggcaa catctacttc acctggccat tcatcaaggt
    22861 tgcgctggtg ctcaactact tcggccaagg cgcgtggatg ctggccaata gcgataaccc
    22921 gcaatacact gcgacggagt cactcaaccc gttcttccag atgatgtctc cgaacgtacg
    22981 ttatctggcc gtgattctgt ctgtttcggc cggcgtgatc gcttcccagg ccctgattac
    23041 cggcgcgttc accatggtct ccgaagccac ccgcctcaat tggatgcctc atctgcaggt
    23101 gcggtacccg gcccgcacgc gtggccagct gtacattccg gtggtcaacg gcgtgctgtg
    23161 cgtctccacg ctggccgtac tcgccatctt caaggattcg gaacacatct ctgccgcata
    23221 tggactggcg ttgaccatta cgatgatcac caccaccgtg ttgctcggcg tccacctatg
    23281 gcattccggc aagcgggtcg gtgccatcgt gttcaccgtg ctgttcttgg cgattcaggc
    23341 catgttcttc atcgcgtcta tggccaaatt cctgcatggt ggttggttca ccatgttgct
    23401 gaccgccgcg attctgttcg tcatgtacac gtggaatgag ggcaccaagc tcgaacgtgc
    23461 gcaacgccgt cacatgcggc ccaacgactt cctacccgcg ctcgacaagc tgcacagtga
    23521 tttccgcatc ccgtattttg cggacaacat cgtgtacctg acctccgact cggaaaccaa
    23581 gcgactcgac acggacatct tcttctccat cttcgccgac caccccaagc gcgcccgtgc
    23641 ttggtgggca gtcagcgtgg aaaccaccga cgagccgttc acccgtgaat actcggtgga
    23701 ggacttcggc acgaactaca tctaccgcgt gcgcttccgt ctgggattca aggtttcgca
    23761 gtccatcccg gcgtacatcc accagatcat gcacgacctg tccaaaaccg gcgaactgcc
    23821 taagcagaag tcgatttacc ccaaggtcga cgccgatccg gacatcggca ccatccgtta
    23881 cgtgctgatt cacaaggccc tgatgccgga atccaaggtt tctgcacgcg gtgccctgtc
    23941 gttgcaagcc aagtacgcaa tccgccacat ggccggctcg ccggttaagt ggttcggtct
    24001 ggcaccttac aacccgttgg tcgaggtgca gccgctgttc gtctccaccc gccgcccacc
    24061 gcgtctgaag cgcaccgata cggccaagac catcccgacg cccacgccga ctcgtgcagt
    24121 ggccgacccg gccgccgtcc ctgacccgat ggacaccacg tccgggcttg ggcgattggt
    24181 acaggagctc gatgccgccg tatcggccga ggcgcggaag accgctgagg ccgctgccgc
    24241 cgatgcgccc gctgagcagg gcgataaggg cgataagggc aaggccgaga acggcaaacc
    24301 cgccgccaag cctcagcgct ccgccaaaca gaagcgataa cataccagtt tgcagcggcg
    24361 ctgatgcgct ctataaagaa tgcctataac ggtcgccgca cgtcgccaca cggtttcggt
    24421 aagttgaaat accatgtgca gattactggg atacgcgacg gcaggcttca acctgagtct
    24481 caacgctgtg ttggggcaag acaatgtcga ggatttccgt gaactgtctg aaatccacaa
    24541 tgatggctgg ggtgtagccc agctttccga tcctgccgaa gcaccgcacg tgcgcgacgg
    24601 tggcgcaccc accccggaaa ccggcacccg aatctacaag tcgaccatcg cggcccgtca
    24661 tgacagcacg tttgaggcgc tggccgacga accgtctcgc ggtgccatct ggcacctgcg
    24721 cttggcaagt tccaatctgc cgctcatcat ggagaaccag cacccgttct atgcgaacgg
    24781 cctgagcttc atccacaacg gcgatatctc ggacgccaat ggccgcaaca tcgtcaccaa
    24841 ccgctcatac ccggtgaacc acagcgtgtt cctctccacc ggcggccgtt ccgactccgc
    24901 catcttcttc gcggtgattc tggaatacat cggcttcggc ttctcgctcg acgaggccgt
    24961 ggcccaggcc gtgcgcgaac tgcgccaagc ctacccgaag tccagctaca actgcatgat
    25021 tcagtccgag gaccagctca tcgccctgtg cgccgccggt cgtgagaaga ccagcccgcg
    25081 catcgtggaa atctacgatg agtacggccg tggcgagcag gccgccgact accgtgtgat
    25141 gcgctaccgt gagctgcgcg acgacaacgg cgactccgcc ggtgtggtgg tctcctcttc
    25201 cggctacaag caggaaggct ggaacgtgct ggagaacgac cagatgatca tcgtctccaa
    25261 ccgcaacggc acctaccgtc tccgttccat ctgaaatacc ttcataagcc ccctctgacg
    25321 agagggctgt cagccgacag gctgacttgg ggggagaccc ctagagcctc aaatcatccg
    25381 catcccatac ttctccgcca acgccggaat atccggcacg ccatacttgg ctaccagatc
    25441 cagccatcgc tgtttgttct ctccgccaaa tcgctcggct cgggccgtat acgcctcgac
    25501 ctgcaggaac ttcggcggca ctgacttgct atggaacttg tcggcgtaca tcaccacttc
    25561 ctgctcgaga ttcatcggca catagtcggc tggtggcaac ggcagctcct ggcgcaccac
    25621 atcctcgcgc gtaagtccca cgccggtatg gttgcggcag aactgggcga tggactcgtc
    25681 cacgccctcg tcgagcagat actcgtagcc cttaagaccg tgcagaatgt agcgtttctt
    25741 gctgaatttg agcggttcgc cgtcagagcc gtcatgcttg aacacgcggt aggtgccgat
    25801 gtcatgcagc agtccgccga tgagtaccaa atgctcgtcg agcagacgcg gcggcacatg
    25861 gccgccggtg acgcctgcgg tgggcggtac ctccgcgtcc ttcggtaatg tgcagcggcg
    25921 cgtaaacagc gcgttctgcc ggcgcacgat ctggcagccg atggtcgcta caatcacgca
    25981 atgcgtgtgc accagctcgt acgccgcctt ggacggcgcg attttgcggt gcaattcgtc
    26041 gatctgctcc agtgtgggaa tatatccggt catgattatc aggtttcgcc tcgtacggtc
    26101 agatttatcc tcagattagc gcggcaatga gccgcggcat tgaaaaaccc ctgtgattcg
    26161 gcaatacgaa tcacaggggg ctctatggtc tgaacatcag atttcgaaga gtgtgtcggc
    26221 tgcttcggcg atttcgcgcg acgcagtgag cacgataacg cagtgcttcg ggtcaccggt
    26281 gtgcgccagc ttggccagcg cggcgaacac ggtcacctcg tcatccgcgt tcagccctcg
    26341 tgtcggctcg tcgaggatca gcacttcggc ttcggtgctg attgcacggg cgatggccac
    26401 caaacgctgc tgtaccaaag gcaatgtgcc gacggccacg ccgctggtgg cctcagagaa
    26461 gccgaccttg gccagcagtt cgcgggcgat taccggcttg ggcttcagga agttgcggtt
    26521 ggacgcattc atggcgtaca gcacgttttg ttcggcgtcg atcttcggct gcacggcgtg
    26581 cagctgcggt acgatgccca gacggtggcc gcgcagttcc acgggctcga gctcaacaat
    26641 gttggcgctc ttgttcatca ccgtgccaga cgccggcgtg gtcaaaccgc tcatcacgcc
    26701 catcagcgcg cgacgcttgg cgtcggcggt gatacccgaa tcggggtcgt cgctgatcag
    26761 cagcgcgtat gcgtgtccgg aatagcagaa gaaattcgcg ccatcgagga tgttgacctt
    26821 gcccttgggt ccggcgaccg tcaccttgtt cagggcgaag gtcgggtagg tcttgagcag
    26881 aatgtcgtcg gaaccgccgc ccaccaacac gcgatcgata cgtgaggcca gcgtcatggt
    26941 gggttcggac gctttgccct gaggctccgc atgctcggcc aatgcgtctt tgtgatcggt
    27001 tttggccagt cggagtgttt cgttgacgcg ggcggcattg cgctcggtca cttcggcgga
    27061 tgcgttggcc gaagcgatgg cgtctgcttc ggcctcgctg gtctcgtcgg tttctccggt
    27121 ctcggcagat gccagtgcgc tgtaaatacc atcgtcaaca gtgccttcgt catcaacgtc
    27181 atcgaaggtc acgctgaact gcatggagtc agctggaacg gattcaatgg cgtcgttcgt
    27241 ctcagtctca gaagtcgctt cgtcagccgt tgcgtcagtg gtcttctcga gttcctgatc
    27301 gttgttcctg tcgctcatgc ctgtgcctcc gcatcgtcgg ttgtggtggt cgtcttgttg
    27361 gtctcgctca cggtgcccca gtcgccatcg gcggcaaaca gcttgtccgc gctgaagaac
    27421 gcaaggcgca gggcggcaag aatcgcaagg atcaggattg aacccagtcc ataccagacc
    27481 atggtccaaa tcaggcccga tgtcaccggg gtggcatggc cggatgccag ggcagatccg
    27541 atcggattgg cggcgaacgc gcctccgacc agaccaatca cgtagaaggg cagggtgacg
    27601 atgaacactt ccagcatgaa ctgccatccc aaacgcccgc gagtgacgcc ggacaccagt
    27661 gccatgccga tctcgtcgtc acgcttgggc gcccacacgc ccgacagcac gaggatgagc
    27721 aacagcacgc cgcccacggc aaccaacgca atggtgccgg tcttcgcgcg cgaggatagc
    27781 gtatccagcg gctccagaga agcgttgtag gattccagcg aaggggaaga gaactcgtaa
    27841 cccttggcgg gcagcttgga tttcttgagg gtggacacca aggacttgta tgtagcggaa
    27901 tcaccggcgt cgaacaccac gtccagatcc gggactgccc agccggagac ggtcttgtcg
    27961 gtggactgtg ggtcgagtcc ggccttggcg aaggtctgat agttggtgaa aatcgcgttc
    28021 tcgcggttgc gggcggccgt caccggattg gcgacggcgg attcggatgt gtacttgtag
    28081 atgccgcgca ccttgagttt ggccgtggtc ttggcgtccg aggcggccgc caccttgagc
    28141 gtatcgccca ccttcaggtt gttggcggag gcaaacgctt cagagaccag tgcgccggtg
    28201 gtgttcgggt tggaggactg gttgttgtag ctcaggtcct tgccgtccac gatttcgaag
    28261 gtgccgaggt cgctggcctt ggcggaatcc ttggtccaga aggctcgcca aatgagctgg
    28321 ccgccggtct cgtcatcgga ggccgagccc agcgatccgc cgtccagagc cttcaaatca
    28381 ccgctcacgc gggccggcac ggtttcggta accgtgaagt tcaacgagtt ggtggccgac
    28441 tggacgagcg tggcgtattc ggtgtatgag gtccacgtca tgtaatgctt ggtggaagcc
    28501 gagtccgtag cggagacctt cttccacgta gccgcagtcg ggcgaatggc caacgtggcc
    28561 ttctgctgat cgtacgcggt ggtgtgtgcg ttggtatctt cttgaatgat ggccagtccg
    28621 gcgactgtgc cgaaggtgac cgccaaggtg atgaacgcgg tcagggccgt acgtccttta
    28681 gaacgtgtca gggcccgcca ggcgttgctg aggacgaaca tgtaattacc tctcccgttc
    28741 tcctaagccg aacaaatcaa tacggctcta actattgttg cgtttagtga ccgttttctt
    28801 gcgcaatact gttatacccc tcaatgaagt ctgagaacat tctgacagcg atgtggtggt
    28861 catgcgggga tttgcaccca gtatgggata aatcctcggt ctatttcggt ggcatcggta
    28921 cactggcatg agatatgcgt ggcgtcacgt gtgctccggc atgtctgcga ttccggattt
    28981 gcccggagtc cggcatccgg aagcgtggcc tcgcgccgct ggataagagt atgagctgag
    29041 cagattttcg ggcagggtaa gtatgcgatt cacttcaggg cattcgagcc ataaggctgt
    29101 ggggcgtagg agaagacgtc atgccggtaa cggaaaattc aatcgctccg catctacgaa
    29161 tagcgctgca aatcatcaga cgggtcccgt aaacctgaag actccccagc acgtgggtgg
    29221 cgacggccgt tcgctgatca gccgtacgcc gatatgggcg cgagtggtgg ttgtactgct
    29281 gctgacgttg ctggccagcg tcacctgtgt gggcacgctc tacgcggcat ccgtctcgag
    29341 aatggccacg gatgcgcagc gcgtgctgac atccgccgaa agtctggcca attcggcact
    29401 tggctgtggc agcgataaga gtctgtcgga catctcccaa gagctggtca atgccaccaa
    29461 tgatctcaat gcggagctga acggcccaca gtgggacttc ttccgcgacc attcgcgatt
    29521 cggaagcgac atcaccgccg cccgcgaaat gctggcttcc gtggatacgc tggtcaacgg
    29581 tccgttcact gatctgctga acctgtccaa acgattgcag ggcttctccc tgaagaatgg
    29641 ctcggtggac gtcagcgccc tgatggatat gccggatatc gtcaagcagg cgcataagga
    29701 tatctcgcag cagctcacca agctcaacaa ggtgccgact ccgagcgtcg ccaaggtggc
    29761 caccgtgctc gaaaccgaaa aggctgcgct caagaccgtg gattcgatgc tcggcgaata
    29821 cgatggcctc attaacttgc taccgcaact gcttggcgag gacggcaagc gcacgtattt
    29881 ggtgatggtg cagaacccgg ccgagctacg ttcggcgggc ggcatggtag gcaccatcgc
    29941 cgcaatcacg gcagataagg gcacgattac catcggtgat ttcgccacca cttctggttg
    30001 ggacattcct gaggagccaa tggatgatac ggtgctgaag gagcgccagg tatttggtgg
    30061 cacttttgac cagtacccgg ccaccacgac catcgacccc gaattccagc gtgtggcaca
    30121 gatgaacaaa tacatgtggc tgtatcagaa aggcaacgaa gacgagaacg tagcgggtgt
    30181 attgtcactc gatcccgtat ttctccaggc attgctggga gccacaggcg aggtcaagct
    30241 ttcggacggc cgagtgctgg atagcaccac cacggtgccg ttcttcgctt cagacctgta
    30301 cacggattac ccggacttcg agcagcagaa caacttcgtt tccgaagcgg ctcaagcaat
    30361 tatgaaccat gtgcttggca acgcgaatgc ctctaccgca tccccgctgt tgaaggcaat
    30421 tagagatacc agcgctagcg gtcatttcaa gctatggatg gctgatccgg atgagcagga
    30481 ggcgctgatt gccaccggac ttatcgacga taaggccagt ggcgagttgt ctgctgacag
    30541 tcaggtgccc gaagccggca tctatctttc ggagctgcag caaggcaagc aggattggta
    30601 tctgaagacc tccaccacgg tcacaaaaac ctgcggcgac gcctcagcca gccagaacgc
    30661 cctgtattct ggcgtgctgg ataagcgcat cacgacagcc gtgcgtaaca cgcatctggg
    30721 tcagtttacg gaagatcagc tcggtgacga atacacggtc accttcacca tgaagaacac
    30781 gctgactaag gctaaggccg aatcgttgcc tgatttcgtt aatggcggct cggagaatcc
    30841 agtgctcggc ggcatgttgt accgcgtggt gcttaccgct ccgtacggtg gcgagatcac
    30901 cgctgtgcag gctgatatag attcatgggg taccaacacc gcgagcctgt atgaccgtca
    30961 gtacattatg ttcaaccagc agtggatcga gcccggaaaa gaattgacga ttgcttatac
    31021 cgttcgggtc agcagcgatg ccacacatcc actgaacgtg gtaaccacgc cggtggtgaa
    31081 tgcggatggc gtcgagacgg gctccaacgg taatgtgacc gatgagtgca cggcggatac
    31141 gaacggcgcc gatggtgcga acggtgccga tggtgcgaac ggcggcgcgg acggcggcaa
    31201 gaacgacgcg cataaggacg cgtcctccga tccctcggcc ggcctggacg ccttggacaa
    31261 gttgaagagc cagatcagct gcccggtcga tctgaagtcc ctggcgggat ccatgtgatg
    31321 accatgcccg attccgtttc cggcgtcgcc ggcaagccgc gcgacgtgaa ctacgatctg
    31381 ctgcgcgtgg tggcgatgtg tctggtcatc ggcgtccacg tgatcaacaa ctacatgccc
    31441 gtcaagatga ccctcgccgg cagcgcggaa gtggtgcggt ttgtgctgtt cgcattgttc
    31501 atggtctgca atccgctgtt cattatggta tcgggccggt tcaacctgac attcgatgcc
    31561 gaccggccgg tgagcaccca ctcagcgggc cgtctgccgg gctctggctc tgatgcccgg
    31621 gcaggcggcg gtgcgggcca agccgatccc ggtagcctcg cgccgtacgc gcgctactac
    31681 tacaaacgct ttgtctcgct gattgtgccg tatctgctgt atgcgggtgg catgggattc
    31741 gttgcctacc tggtgattga tcatcgttcg gttggcggcg ctgtgtctgg aacgttgttc
    31801 gatctgttct ccgggtatga cgattccgtc tattggttcg tgttcatgct tgccgggttt
    31861 gtgctggcaa cgccgttcct ggccgcgatg atgcgtacga taggccgatc gggagcctgg
    31921 ttgctggtcg ggctggccgc cgccgtggcc gctgccgagc atatatgcga tcttgtcgga
    31981 tatccgttga cgttcctgca gtcattccct tggcgtggac tgctgatcta ctacctgttg
    32041 ggattcgtgt tggaatacta tccgccgtcc gcgcgtatac ggcgcggcgt gtatgcgttg
    32101 gcaccattcg ccctggcctg gacagtcgcc acgccatatc tgttcgccgg ccagcagatg
    32161 caggttggcc gcacggtcac ggttgcgttc gcgatggtgg taatggcgac gttcctgttc
    32221 ttccggtacg acgtgcacat tacgtcggcg cgggtgcgta aggcgattat ctggctggct
    32281 ggatattcct acacgattta tttggtgcac tcgccactgt ccaaggtgct gattggcccg
    32341 aggattcccg tgcctacgga cggatggtcg tatgcgggta tcagcgtgct gatgtttggc
    32401 gctacgttgc tggcggcgct gctatttgcg gtgattgccg ataccgtggt gcttaagccc
    32461 gtgcagcgtc tgctgcggaa ggtacggctg tagattcgga tacggcgcta tgccacggta
    32521 tcaaagccag gaatcccagc agcagaagcg gagtgaacgt gaatttcgac atgcccatca
    32581 gcgcgcccac ggtcatgagc gcggccaatc gtccgttgcc ggcgcgcggg tcggccatgc
    32641 aggaaaacag caggcacgcg aacaataggc acagcgcctg tgccatggaa tcggccgaga
    32701 tggcgaactg aatccaatca cggctcacgc acccgcccac attgccgctg cccgaatgca
    32761 ggatgctggt cgagtcgacc ggccgagcca gcacatcccc gttaacaatg ccgtcgatgc
    32821 gatacgtgtg cgcccatacg tctggaatat gcccggcagg cgtcaccgca atcagcacga
    32881 aaccctcgcg gcactgcgga acacggaccc caagggccag ctccaccgcg cctggcagct
    32941 cagggaactc ctccgcaccc tgctcagaca cccgctcgaa caggccaggg gcgagttgaa
    33001 acactgggtg ttctgggcgt cccacagccg catccccgag atcacggaac tcgccaggaa
    33061 gattcgcagg cgccggcccg acatcctcag gaccatcgag ctgggct
//
LOCUS       DAWBMW010000005        27977 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_491_length_27977_cov_3.129575, whole genome shotgun sequence.
ACCESSION   DAWBMW010000005 DAWBMW010000000
VERSION     DAWBMW010000005.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 27977)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 27977)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..27977
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_491_length_27977_cov_3.129575"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4668..4677
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    20127..20136
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    22467..22476
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    25564..25586
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5408 a         8493 c         8561 g         5462 t
ORIGIN      
        1 cctgcgtgcc gtggacgacc tcaatctgac cgtgccggaa ggcgaatggc tcgccatcgt
       61 cggctcctcc ggctcaggca agaccacgct gatgaacatg attggctgca tggacacgcc
      121 ctccaagggc tccgtgaagc tggaaggccg caagctggag gacctgaacg ccacgcagct
      181 ggccgacgtg cgcaagaacc tcatcggact ggtgttccag aagttctatc tggtgccgca
      241 cctgaccgcc gtggaaaacg tgatggtggc ccagtactac cattcggtgg tcgatgagaa
      301 gcaggccatg gaggcgctgg aaaaggtcgg cctgaaggac cgcgcccacc acctgcccgg
      361 ccagctctcc ggcggcgagc agcagcgcgt gtgcgtggcc cgcgcgctga tcaacgaccc
      421 gaagctgatt ctggccgatg agcccaccgg caacctcgac gagaagaacg agaaaatcgt
      481 gctcgacctg ttccgcaagc tgcacgagca gggcaccacg attatcgtgg tcacccacga
      541 tgcgctggtc gccagctgcg cgcagcgcga gatcatgctc aaccatggcg tgctcgtcgg
      601 tgagaagtgg aatgacgagg atgcgcgcaa ggcctacgag gcggccggcg gcaagcccgc
      661 ctccacgggt gcacaggtcg agggcgcgca gaacggcgag accgccattg gtttcactga
      721 ccccaccaag gccgcgaaaa ccggcggcga ggagtagggc cgtcaggggc atcgagcagt
      781 atgtgaggaa tacatggcaa gctgatggtc tctcccccag tcagcttcgc tgacagcccc
      841 cctcgtcaga ggggggctta aggagcgtga atatgaccga aaagagtacg ttgaagaaat
      901 ccgcgacatt ggcggcggcc ggactggtcg tggctggcgc gctgctggcc ggatgcggcg
      961 aatccaaggc cgcgccgatg gacgatgcgt acgccggcaa ctcgggcgag accgagaagt
     1021 cgcaggaaga gcagtcgcaa tcccagtcga acggctccga tagctccgac ggctccgatt
     1081 ccagctcgtc gcacaacgac ccgtccacca ccacgtccga caagaaggac accggcaact
     1141 acaaggacgg caactactcg atcaacggcc agtacggccc ggtaggcgag gattccatcg
     1201 acgtccatct gaccatcaag gacggcaacg tcgagaccgt caatatcatt gggcacccgt
     1261 tcaccacgat ttccaagaac catcaggacg acttcgccaa agccattaac ggcgtggtgg
     1321 acggcaaacc tctgaagggt ctcaaagtgg acaaggtcgc cggcgccagc tggacctccg
     1381 acgccttcaa caaagccctc gaagtcgccc gccaggaggc ttcgattcag gagtaagacc
     1441 gctacttcag caatgctggc cacagcagat cggccatgcg cgaggcccag gcggtgtcgt
     1501 cgtccattgc tttgccctcg atggcctcat acgccaccaa agatccgata atcgtggaca
     1561 acagcaactt ctcgtcaacg tcctcgcgaa acacgccctg cgcgactccc tgcttgagga
     1621 acgcccccac acgctccagc cacggggtca ccacttggtc gacgatgtgc tggaagaact
     1681 cggcctcgct ggacaacaca atgcccacgc gcttgacgtc gatgtcgttg tcgcgcaccc
     1741 aatcgaccaa tcgctgcaat agttgcgtga aatgctcacg cgtggggctg ggcactgggt
     1801 cggaatccgg gatggccaga tacatcgcgc tgatctcgtg cagcagctcg tcggcattat
     1861 gatatcggcg gtaaatcgtg gttttggcca caccggagcg ccgggccacc tcctcgatgg
     1921 tcacgccgct gatgccgcgg tccaccgcaa tctgcagcgt ggctcgcatg agcttgcgat
     1981 ccgtcgcctc gcgcgtgcgc tcaccgcgcg gtgtcgccgt cgtatctgtc atctccgtca
     2041 tctcttctct tcgaaatatg ctcccgtacg ctcccgctgg cgagagctgg ctggcaaagc
     2101 cagactgagg gtggtaagtc catgccgttt gtaggcaact cccggataat acctactagc
     2161 tcagcacttt agtgctctcg acttgggcta cataccactg gttgaaccgc accagtggct
     2221 tcctgagcaa cagcccaagc aacagtagcg gcggcacgaa tgccagcagc attccggcgg
     2281 cccgccagta atccatgtcg tagatgccgg cgatggccgc acgcatcatc gtcaccgcat
     2341 gggtgagcgg caagagcgga ctgactttgg cgatgaagtc gggcaacatc tgcaccgggt
     2401 aggcagcgtt cgcacccgaa atctgcacga tgagcataag cacgccgatt gccttaccca
     2461 cattgccgaa actcaccacc atcgtatagg tgaagaacga gaacaccagc gaggccagcc
     2521 atccagacaa cataaacagc agcggatgca cggcctgcac gcgcaggaac agcaaatcgc
     2581 caccgagcga caccgtggat tgcagcagcg caatcagcgc gaacaccccg taatgcccaa
     2641 ggaagagccg atgcggccgc ggatcgccca atgctttgcg cgtccggcga gacacggtgg
     2701 ttttcagcgt caccaccatc agcagcgcgc ccacccacag cggcagcagc gtgtagaacg
     2761 gtgccatcga cgaaccgaaa ttcgccaccg ggaatacggc cttgcgcctg agctgcaccg
     2821 gtgcggccaa cgtactggcc agcgtttcgg gatcattgcc cagcacctct ttgaccatgc
     2881 ccatatcgcc actgtttagt gcgtcgctca gtgtgctgtt gaacgaagcc agttcggcac
     2941 cggcggaacg cagtttgccg gacaccttgt ccaaggtagt gcgagcgtca ctgagcatat
     3001 cgctggcgtc gtcggaagtc cctttcaggt cgccgagcgt ggagtccaac tgggtggcgc
     3061 cggaactcag tatgccggag gcgtcagcga cggtggacga cagggagtcg atttgcggtt
     3121 tcaccgtggc cgagaaatct gacttgatat tggcaatact gttctgcgct tgatcagcca
     3181 gattcttgac ctgcgcatgc tgatcctgcg cgtcctgatt gccttggcga atatcggtcg
     3241 ccgccgtatt cagcgcttgc cgcagcctgt tttgccgtgc gattgaacgg tcaatcgccg
     3301 tgatgatgga tgagtcgttg cccagtatgc cggccaacgt gttgcgaatt tcagtgtatt
     3361 tctccgtctg cttgccaatg gccgtggctt gacggtccaa ttcgtcggcg accgtggtgg
     3421 caccatcatt cgcgtcggaa tacagcttgt ctatggaatc ggacaccgcg ccgaaactgg
     3481 aggcgctcgc gtccagtgcg ctgctcatcg cgtcagcggt ggtgcccagg gctccggcca
     3541 cgtcggtgac ttcctgtttg gcgctggcaa gatccttgcc gccttgtttg gcggcagagg
     3601 aaacctgagt gatcagtgag ttcgaggtgt cgagcaaggc gccggccgta tcggtcaatg
     3661 cgctgtatgt gccaagcaga tccgccgtat cgtcaagctg tgtggcgaaa ttggcgatgt
     3721 tggcgttgaa ggtattcagt tgctctttgg tctcgggctt ggacagctga tcggccagcg
     3781 aagacgcgat ttccagcccg gccgaagtga tggtcttagc gaacgtggtg ttgatttccg
     3841 ccgcaacctc atccgtgccc tcgttgagca gattcggtgc cagcgcgttc ttcttctcgt
     3901 tgcggtaata ggtcagttcg gcatgctgca cgtcgtcgga gaagaaggtc atcatgtctt
     3961 tgctgaacga cttcggaatt acgatggccg cgtagtattt gccggatcgt gcgccctcga
     4021 cggcatcggc tttggacgtg atcgtccaat cgagctgact gttggcgcgc agcgcgttca
     4081 ccacctgatc gccgaccgtg actttcaccg ggatcaggtc ggaacgatac ccctcatcca
     4141 cattcgccac cgcgaatttc aggttcttca tattcgagaa cgggtcccag catgcggcca
     4201 cgttgaacca ggtgaacagg ccgggaatca tcaccaagcc gatcacgatg atgatggaga
     4261 ccacgttgct ggtcaggcgt ttgatgtcgc cggtaaacag tttccacatg gtgttcatcg
     4321 tgcgtgcctg ccttccttgg tcggcggaat agtggggatg attgcggtaa cggcggcgct
     4381 cgggtccgct tcttcgtggc gttcggcgga ggtgtcgcga cgcttggcct tatgcccgta
     4441 cagcatggcg cggatcgact cgtcggagag gttgccgagc ttggtttggc ggcggatgga
     4501 atcgcgcatg aattccacga ccatcaggaa gcccatgatg atgagcaccc atgccagcca
     4561 tgtgcccatg actacgattt tctcgctggt ggtcagcgca aacacgataa tgagcgcggc
     4621 cggcaccacg aatccggcga taagcgcacc cagcagcagc cggggatnnn nnnnnnngga
     4681 tgacggtagg cgaatttggc ggcccgacgt tcgatggcat gacggtattc gtcgtggtcg
     4741 gccagtgctg caatggcctg agtgacacgg tattcgctgc cgggcatgtg caccggctcg
     4801 ccgataatca tgtcgctttc cttgatttcc cgcgcgaaca ggcggttgag attggccagt
     4861 ttcggccggg cgccgaggcc aagcgcaaag gccagaacca cgaaaatcag caatttgccg
     4921 atataggcga accagaggcc gtcgtagaag ccgccgatgg tttcacgcat cgcgtcaatc
     4981 gaataggtga acggcagcag cgggtagatg gcgcggaaga acgcgggcat catttcaatc
     5041 gggtacaggc cggaggcgcc cggcacctgc agaatgacca gcgccacgca tacgcctttg
     5101 cccacgtgca tgaacgtggt ggagagggcg aaaatcaccg acatatacac cagtgaggtg
     5161 atgacgcagg tgagcagaaa cactggggcg ctcgcgcatt gaatgccgat gacaagatcg
     5221 ccgatggccg tgaccaggcc ttggacggca gccagcgtgg ccagcagcag gaagcgaccg
     5281 agatagcctt gggttggcgt gagatcgtcg atgccctcat cgtcggtttc gagtttggga
     5341 atgaccacga gcacgaacgc gcccacccac aaagacaggg tggtgaacag cggtgccatg
     5401 ccggaaccat aggaggcgac cggatagacg gcctgttcgc tgataacagt gggggagagc
     5461 atgaagtcgg cgattgtggc ggcatccagt ttgccgtcgg tgccgatcag ggaactgagt
     5521 gaactggaga tgctcagggc tttgatgtcg gtgaccagcg tatcgagctt ggtttgtacg
     5581 ccggccagcg ccttatcggt gtcgcgcagc gcggtcaccg tggtggaggc tgctttgtcg
     5641 agttggtcga gggtatgctt cgactgatcg atgagtgagc cttgcgacgt gatgccggtc
     5701 gacagagtgc tggcggtcga gctgagggcg ttgagaccgg tgttgagctg aggaatggcg
     5761 ccggaggtga tggtgccgcg agcggtattg gcttcgcgca ccgtggtttg ggtggcggta
     5821 ttgagatgct gcgccaatcc ggcggtgtct gtggaggcat tgccgatagt ggtgttcagg
     5881 ctatcgaggt tgcccagtgc gccggcaagc tcgctattgc ggctttccag tgtggcaatg
     5941 gcggattgca gcggttctcg attggcgttc ggcagccctt tgagttggtc gatgatgtcg
     6001 gcgttcgctt ggttgatgtc ttccgccgta ttgatcagtc cgcctacctg ctgattggcg
     6061 gcgctgatgg ttccggttac cgtgcccacg ctttggttgg cctgtgctgc ggcttggctc
     6121 agcaggcttg agccttggtc aagcgcttcg gacgtggagg tgacgaagcc gttcagactg
     6181 gtctgggtag tgccgatcag ggtggagacg ccggccagac cttctgcagt gtcgatgcca
     6241 agtgtgcgtg cgtcgtccag cgcctgacgt gcggtgcgtg tctgctctgg ggtgtcggtg
     6301 agtttggtgg tgagcttggc gatggtgctg cgtgtgtgct gcacatcgtc ggaggcctcg
     6361 gcaagggccg tgagggcctt ggctttggtg gtgtcttcgg tactgatggc tttgccgtca
     6421 accgtattga tgacttcggt aagtacttcg gatacggtgg agacgaatgt ggaattgacc
     6481 gtgcggtcca cagtggacgc gccgacgtcg gtgactttgg gggcgatggc gctggctttt
     6541 tcgttgacgt aatactccag agtgggccga ctcttgccgc cggtcaccac gccggcgaga
     6601 tcgtcggaaa agtctttggg aatgatgatg gctgcgtatg ccttgccgga ttccacttgg
     6661 tccatggcct cggcttcggt gacgaaggtc cagccgagct ggtcgttgct tctcatctgc
     6721 ttgacgattt gatcgccgag gttttgcttg ccgatgagcg cgttgtcggt gccgtcgtca
     6781 ttgttggcga tggcgacggt gatgcctttg gtattgccgt acgggtccca gaagccgacg
     6841 atgttgtacc acgaatacag tggcgggatg aacaccatgc cgaacaggat cacccatgcg
     6901 gccggcacct tgaacaggcg caggaagtcg cgtctgagga ccttgaagat attgcccact
     6961 gatacctccg gagcttggtg ttgcttggtt gctacgtttt ccgtagcgat acgttaatcg
     7021 tagcgctacg tttgtagtag cgtaatacgg ctgccggaga ttaatggggt gattgatgat
     7081 gtttttggcg gaataacgcc gctgcccagc ggcttatcgt gtgtcgctgg gcagagcaaa
     7141 aggcgttctg gaacttattg acaagatttc tggatggttt ttggacgttt cctgaacgcg
     7201 atggataggc tcggtgctcg gcaatgtaga cagacatgta ccgaccgccc atcaagttca
     7261 gcaaaaccgc aatcgggtga tagggtgagg ggacgctatg tggggaggaa accatggttg
     7321 aattcaagga cgatggcacg gccactaccc ccggcaagcc gtgggagaac cgtgacgccg
     7381 agtccgcagt cgtggccgat ctggtggccg tcgaactgga cgacgccggc ttgaacctga
     7441 acaccaagcg attaccgatt ctggcgcgca tctatggcgc ggtcgtgctg ctggacggac
     7501 tcgccacatt gccgattatg gtgatttcca tcctctatgc cgtgcgcgag attctggatg
     7561 gtcatgtgca cgtcgatgcg atgagcctga ccttcatcct gtccgcgatt catgcggtgg
     7621 tgctggtagt cagtgccgca tgcctgattg tgttcggcgt gatgctgctg cgcaatcatc
     7681 gccggtatgc cgcgcgctgg acctacgtgc tgatgccgtt gacattggcc gacggcatgc
     7741 tgtcgctggc cctgaccggc cttggcttca acctgctgct gccgctgatt cagatgatta
     7801 ttctcgtcgt gatttcggtg accgccgatc cctccctgag cgaggaacgc cgtctgcagc
     7861 gcgcgctcag gcgtatggac gatcgcgatg attatgaatc ggctgtggcg gccggcatgc
     7921 taggccgcga ccagtccggc aagggctata ttgcgctgga ctttttcaat atcttctggc
     7981 tgtttaccat cgcttcggta ggtggcctga ttgtggagac gatctaccat atggcgctgt
     8041 ataacggtga acttcaggac cgcgctggtt tgctgtgggg cccgttctcg ccgatttacg
     8101 gctgtggtgc ggtattggta accgtgtgcc tgaatcgtct gtggaaggcg aatccgttct
     8161 tgattttctg cgcttcggcg gtgatcggcg gcgcgttcga atattgcacc agctggttca
     8221 tggaagcggc gtttggtatt accgcgtggg attacaccgg ccgttggctg tccatcgacg
     8281 gacgtacttc gggccaattc atgttcttct ggggactgat tggtgtggtg tgggtcaagt
     8341 ggctgttacc gcgactgctg gctctgatta atcgcattcc atggaaagtg cggtattcgc
     8401 tgactgcggt atgcacggtg ctgatggtga tcgacattgc cttcacgctg atggccttgg
     8461 attgctggta tgggcgcatg gccggccagc cgcaggattc accggtcgcg atgtggttca
     8521 gcgaacgcta cagcgacgct tacatggcca atcgcttcca gaccatgcat atcgaccctt
     8581 ccaaggccgg gcgtatgtga atggccggca agtaattacg cttcgcgggc agaaggtgcg
     8641 tagaacctaa gacagccctc gcgtgtgggt ggtaagcggg atacactttc aagcgttgaa
     8701 ggaatgattc cctccgctga tgcgctgttt tgtgacgcgc gtgatgacga tgggacaatc
     8761 aaacgaaagg caaggcacat ggccgaagct acagcaaaca ttggtgtgat tggactggcc
     8821 acgatgggct ccaatctggc gcgtaatctg gcgcaccatg gcaacacggt ggcgttgttc
     8881 aaccgtcatt actcgcgtac cgagaagctc atgaacgagc atggcaccga aggcaacttc
     8941 gtgccggccg aaacgctcga ggaattcgcc gcgtccctga agcgtccgcg taccgccatc
     9001 atcatggtca aggccggtgc tccgaccgac gcgaccatcg cgcagctgga agaggtgttc
     9061 gaaccgggcg acatcatcgt cgacggtggc aactccttct tcaaggacac catcaagcgt
     9121 gagaaggaag tacgtgccaa gggcttccac ttcgtgggtt gcggcgtgtc cggtggtgag
     9181 gaaggcgcgc tcaacggccc gtctctgatg cccggcggca ctgcggaatc ctggaagacc
     9241 ttgggcccga ttcttcagtc catcgccgcc aaggtgaacg gcgaaccgtg cgtgacccac
     9301 atcggcaccg atggtgccgg ccactttgtc aagatggtgc acaacggcat cgagtatgcc
     9361 gatatgcagg tgattggtga ggcgtatgac attctgcgcc gtggactcgg catggatgcc
     9421 gaggagatcg gcgacgtgtt cgctgaatgg aacaagggcg accttgactc ctacctgatc
     9481 gagatcaccg ctgagattct gcaccacaag gatgccgaga ccggcaagcc gttcgtggac
     9541 gtggtcgtgg atcatgccgg catgaagggt actggtacgt ggaccgtgca gactggtctg
     9601 gaatgtggtt ctcctgtggc tgccatcggt gaagccgtgt tcgctcgtgc actgtcttcg
     9661 cacggtgagc tgcgtgaaga cgctcagaag gaaggtctgg ctggaccgaa caagaccatc
     9721 gatctggccg gagaagacaa ggccgcgttc gtcgaggacg tgcgcaaggc tctgttcgct
     9781 tccaaggtcg tggcctatgc tcagggcctg aacgagattc aggacggtgc caaggaatac
     9841 ggctgggata tcaacctgtc cgaagtggcc cgcatttggc gtggcggctg cattattcgc
     9901 gcccagttcc tgctcgaccg catcaccgag gccttcaggg gcgataaccc gccggcttcc
     9961 ctgctgttcg atccgtactt cgagaagatc atcggcgaat cgcaggatgc atggcgtcgc
    10021 gtgatcgttc gcgcggtgga ggccggcatc ccgaccccgg tgttctccag ctccctggcg
    10081 tactacgatg gtctgcgctc caagcgcctg cccaccgctc tgacccagtc ccagcgtgac
    10141 ttcttcggtg cccacaccta cggccgcgta gacaagcccg gcgtgttcca cacgctgtgg
    10201 gctgaagaag gcaagcctga aatcgaggcg tagcctcgtg atttcaggtt taggacagcc
    10261 ctgtgggctg tccggccttc ttcaggctcg gcgatagccg agctgagaaa acagcaagcc
    10321 cgagatcgag gcgtagcctc gcgatctcgg gcttagggga gcccggtggg ctccccggcc
    10381 ctcttcggag cgaggcggct atgccgccga gccaacactg agcctcgccg caggcgagtc
    10441 cctaattgca ggggccggcg gcgaagaggg caagtccgag atcgaggcgt agccatagac
    10501 gaattaagcc ccctcatcag aggggggggc ttaattcact cacaactcgg tgacggtttc
    10561 ttcggtggag aaccagatga actcgtccac tccgttggcg taggaggacg ggtaatccgg
    10621 gttgttgggc tgctcgaaga catgggccag cgattcggct ttgccggcac cggcggcgaa
    10681 gaaccacgta tggcgtgaac gcgcgatgag cggcgcggtc aatgaaacac gcagcggcgg
    10741 catcttgggg gagtggttca cacccacggt gagcctatca gtaaccttga cctcatcatg
    10801 accagggaac agcgacgcat agtgaccgtc cggtcccatg ccgagaatca tcagatcgat
    10861 taccggttcc ggccccaatt cattcaccaa ctcgcgctca tagtcgctcg cgacatcgtc
    10921 cagcaacgca tcattcgcgg catcggcggc ggcgcgaacg gaagcatcat cgctgtcggc
    10981 ctgatccatc acatcggcga tgtcatcggc tgaacgggta tcggccgcca tctcatgaat
    11041 attcgactca ggcagcttgc catctgccac cagctggtca agcagcaggc gacgggcctc
    11101 caaagcattg cgatcggtgt cgtaagccgg cacaaaacgt tcgtcgctcc accagaaatg
    11161 cacgcgcgac caatcaatcg cgtcagccag cggatcgttc agcaccaagc ccagcggctg
    11221 cgcggccgaa ccgccagaca gcgccacatc cacacgcgtg cgatgcgtgc catcaggcaa
    11281 tcgttcggcg agcaaatcga gtagcgccaa gacaaaacgt tgcgcgccag cctgaatcat
    11341 cagatcttta ttcggataca caatgagttt tcgagttgcc atgtatggtc cttgtatggg
    11401 gtatgtgtcg tactttgcaa tgtggattgt gttgtcagtt gacaacacaa tccacattgt
    11461 gcgacatcgc atatgtcact ttattactcc gcgggcatga gcttcggcat cgcatgaggt
    11521 aggaaacgat gagcaacggc gttggcatgg ctcggccaca attgccgaac tcattccaac
    11581 gccgctgtgg caacgggttc taccgacttc agtgacgaat cagatcccag ccttcgttga
    11641 tgacctcggc gtagatctca tcgggatcga tgcgacgcag ttcctcggtc aggcagtcct
    11701 cgatggtgcg catcggcacg gacacttcct gcggagtctg gccgggcagc gagatcagcg
    11761 cttggtcctc atacgggcgt tccagcgaga tcacaccgtc ctcacgggtc aggtacacgc
    11821 cggtcacggc ctgcgcgtcg ggcacgaact cagtttcgac tggtacgcca agtttcagac
    11881 gcagccatgc gcacagcatt tccataggca ggaagtcggc cttgccggtc accttcgcgg
    11941 cggtgatggg caggtgcggc ggctgatcga gcatcgaggc gagcattgcg cgccagacgg
    12001 tcagtcgtgt ccaggacaag tcgatatctt ccggtgtcca attgcggcgc aaacgctcaa
    12061 tcgtggcctc cggattattg gagtgcaacg cgtcggtgat gcgactgcgg gccatcgcac
    12121 ccatcagatc cttggccggg ttcgacggcg gtgtggtcgg ccaccacgcc actacaggcg
    12181 catccggcac aagcagcggg ataaccagcg tatccgggtg gttgataagg ccaccgcgcg
    12241 gacgcaagat gatgatctca ccggcaccgg cgtccgcgcc gaatcgcact tgggcgttca
    12301 ggttggaatc gttcggatcg ttcgggccgt cggtcaaatc gacggccgga ttatcgcgac
    12361 tcgcgctcac ctcggcggcc acttcggccg cgttcggttc gccttcgccg gactgatcgg
    12421 caggattggt atccggtact acggcaatca cgcggcacgg gtgctcacgg ctggccgagt
    12481 tcgcgatttc cagcgcatgc tctaggtccg cgtcctcggt ggcgatgagc agcgtcagca
    12541 cgcggccggt cgccgattcg ccgcgctcct cgtgcagctg ttcgattttg tgcgcaatct
    12601 cgcgggtgcg ggtgttcggc atatcgatga tcatggcatc ctccagtggc ggccgtcgcg
    12661 cgccagcatt tcgtcggcct gtgccggacc ccacgtaccg gcgcggtagg cctgcggctg
    12721 gccaagcgtg gaccagaatt gttcaatcgg atcgaggatc tcccagctga ggttcacctc
    12781 ttcggtggtc gggaacaacg gcggatcacc gagcagcacg tccagaatca gtcgctcata
    12841 ggcttccggc gagttctcgg tgaaggagcg gccgtagctg aagtccatgt tcacgtcacg
    12901 gacctccatg gtggagccgc ccggcacctt ggctccgaag cgcatggtga cgccctcgtc
    12961 gggctgcacg cgaatcacga tggcgttctt gccgagttcg cgcacggcgg tctgctcgaa
    13021 cggcagatgc ggggcgcgct tgaacaccac ggcgatttcg gttacacgct tgccgaggcg
    13081 cttgccgcaa cgcaggtaga acggcacgcc agcccagcgg cgggtgtcga tatcgaggcg
    13141 gatggcggcg tacgtttcgg tggtgctctt ggggtcgatg cctttttcct ccaagtagcc
    13201 gaccacttcg tgcgagccct gccagccctt ggcgtactgg ccgcgcgcgg tgttggcggc
    13261 gaggtccttg ggcaggcgca cggcggaaag caccttggtc ttttcggcgg tcaggtcctt
    13321 ggcggtgaag ctcaccggct cctccatggc ggtcagagcc atgagctgaa gcaggtggtt
    13381 ctggatgacg tcgcgtgcgg cgccgatgcc gtcgtaatag ccggcgcggc cgccgatgcc
    13441 gatgtcctcg gccatcgtga tctgcacgtg gtccacatag ttggcgttcc agatcggctc
    13501 gtacatggcg ttggcgaaac gcagggccag caggttctgc acggtttcct tgccgaggta
    13561 gtggtcgatg cggaacacgg agctcgggtc gaacacttcg gataccacgc tgtcgagttc
    13621 cttggcgctg gccagatcat ggccgaacgg cttctcgatg atcacgcggc gccatgcgcc
    13681 ctcggacgag cgcgacaggc cggaggcggc cagctgcttg gcgacctgtg ggaatgcgcg
    13741 cggcggcacc gacatgtaga aggcatggtt accctgcgtg ccgcggtcgc ggtcaagctc
    13801 ctgcacggtg gcgcttagac gctcgaaggc ctccgggtcg tcgaacgtac cctgcacgaa
    13861 gcggatgccg gcggccagat tgcgccaagt cgactccttg aacggcgtac ggcaatgcgc
    13921 ctggacggcg gccttgacga attcgatgaa ccgttcctgt gtccagtcgc ggcgggcgaa
    13981 accggtcaga ccgaagctcg gcggcagtag gccacggttc gccaggtcgt agacggccgg
    14041 cagtagtttc ttttgtgcca gatcgcccgt gacaccgaaa atgacgaggc tgcatgggcc
    14101 ggcgatacgg ggcagtctga ggtcgcgcga gtcgcgcagg ggattgctcc aattggggga
    14161 ttcactcata atgtacaagg gtagtcgccg gcggctatct atcgctagag gcgatatgcg
    14221 atttgggctg cgatttgggc cacattgcgt gtgctgtgcc gtgcgttggg ccaatcgcgt
    14281 acgcaaacgt gtacgcaacg ccgtagttga aatatcccaa aacggtgtgt attttagaag
    14341 agttgcccct ttagctcaac ggttagagca gcgtcctttt aagtcgtggg ttgtgggttc
    14401 gaatcccaca gggggcacaa caaatcctcg gaatcgcaat ggttccaagg atttttttgt
    14461 ttcgaccagc ctggctccta gagtggtgtc ccatgtcatg caccacgatt cttgtcggca
    14521 aaaatgccag ttacgacggt tccaccatca tcgcccgcga tgacgattcg ggctcgggcc
    14581 gttacgatcc caagcgcttc gtcgctgtcg cccccgacga ccagccgcgc cactaccgca
    14641 gcgtgctgag ccatgtcgaa atcgaactac cggacaatcc ttgccggtac accatcgcac
    14701 cgaacgtgct gaataatcgt ggcattctgg ccgaagccgg tgccaacgaa cacaacgtgg
    14761 cgatgagcgc caccgaaacc atcgctgtca acgagcgcgt gctcggtgcg gacccgatgg
    14821 tcgagttgcg gccggctgtc ggcgaaccgg acagcaccga ttatcaggcc gagcagcccg
    14881 gcggtatcgg tgaggaagac atcatcaccc ttgtgctgcc gtatgtcacc accgcgcgcg
    14941 agggcgtcgc gcggttgggc gaactgctcg aaacctacgg cacctacgaa tcgaacggcg
    15001 tgattatttc ggatgtggac gagatctggt acgtcgagac catcggcggc caccattgga
    15061 tcgcccgccg agtgcccgat gactgctatg ccacgatccc caaccagctc ggcatcgacg
    15121 acttcgacct cgccgatgcg ttcggcgagc agcgcgaata cctatgcagc gccgacctgc
    15181 gcgagttcat ggccacgcat cacctcgacc gcacaatggg aacgccggtc tcttccaacg
    15241 gccgtcacgc gcatagcgcc ggattcggaa caaccgttgc ccttcccacc cgtttcaacc
    15301 cgcgcaaggc cttcggcacc gcgacgccga aggaccatat ctacaacacg ccgcgtgcct
    15361 ggtacatgca acgccgcctc aaccccagcg aggactggga ttcgccggcc gcccgctaca
    15421 cgcccgaatc cgacgacatc ccgtggtgcc gcgtgccgga agacaaggtg agcctcgaag
    15481 acgttgattt cctgctgtcc tcgcacttcg aaggcacgcc gtacgacccg tacggcacga
    15541 ccggcacggc ggaatcgcgc caccgttacc gtccgatcgg cgtgaaccgc accggccata
    15601 tggtggcgat tcaggtccgt ccgtatgtgc cggcggccaa tcgcacggtg atgtgggtat
    15661 cgttcggctc tggtccgttc accgccgccg cgccgttcta cgcgaacgtc aacgacacgc
    15721 ctgcctacct gcgcgacacc acgcccgaag tctccaccgg caacctgtat tggacgaacc
    15781 gcctgatcgc cgtcgtggcc gacgcacact ggtatgagac gaaccggttc atcgaagggt
    15841 atgccgaggg cgtgcgcgcg ttcggacacc ggctcatcga gcgcacggat gagcagttgc
    15901 ttgctcgaag cgatgccgaa tcgacggata cgaatgcatg gccgctcgat ggcggccacg
    15961 gcgatgagaa ggatgtgcag ggcgtggcgg atgtgctcga agcggccaac gacgagatgg
    16021 ccgactacct gcgcgagcac acaaccaagc tgctgaacga cgtgctatac acctccagca
    16081 acctgatgca caacagcttc gcgatgtccg accgttgggc cgagtagcaa aacctcgact
    16141 ggggaggtaa tagggatgcc ctgctgataa cgcgtcagca gggcaaaacc acaatccgaa
    16201 tcagttaaag ccgacgatgc gttcggcgat gagatagccg ttgcgcacga tccagcggcc
    16261 ggttttgccg ggcagattga gcgcgcggga catcagctgc gagcgcacat ccttgccgat
    16321 ggccggcgaa taggcgtcaa tcgcatccca cagctcggtt ttcttaacgt agttggccgg
    16381 atcgcgcgag agaatcagga acgtcgaggt aacgcacgat tcgatcgcca ggaagtgaat
    16441 catgtaccgg tacagcccct ccggcaccgt accgcgttcg ggcgtggcct cggtcatcag
    16501 ccgattgacg agccgcaact ggtccacacg cttaatcatc acgtcggtct gcacgctctg
    16561 accctcgcgc ccgatgaagt agtggtagaa cggcacatcg aggtactgga tgcgcttcac
    16621 ccacgggaac ggctggtacg agtaatagaa atcgacgtag aacgtgtgct ccggcagctt
    16681 cagcccggaa tcgcgcacca cctgcgtgcg gaaggtgagc gcatgcatga tgatgtactg
    16741 cgccagaccg aacctgccca ggtcatccca gccgagcacg cggccggcct ccattacacg
    16801 gcggaagttc actacggtct tgtgacgctt ggccaccttg tcatacacgt aattggtcac
    16861 cagcatgtca atcggttcgt cggtgtcatg ctgtgcacgc aacgtggcca gaaccgtgtc
    16921 gatggcctgc gggtcgaccc aatcatccgc atccaccact ttgacgtaca tgccggtcgc
    16981 cgccgcgata ccggtgttca ccgcgccgcc gtggcccttg ttctcctgat gaatcgcgcg
    17041 cacggcgccc ggattggtgc gttccagatt gcgcgcgtac tccagcgtgc cgtctttcga
    17101 accgtcgtcc acaatcagca cctcgaggtc gctgatgtcg cttgccgaaa gcagcgagtt
    17161 cacgcaacgg tcaaggtagg actccatatt gtatgcgggg atcacaaagg tgagcgtctt
    17221 tgccgtgctg gccatgaaag tcttacgtcc tcacatattt agtcgttatg ttcagtttcc
    17281 tgttcaaacc gtagcacttc gcctcccgac tcggcagagt gttgcgtaac ctgtgcccct
    17341 gaacgcatgt tctgcaaccc tgaatcttcc ccggtggcgt gctcgggatc cgattgggca
    17401 gaactcgtct ggtctgaatc cgggctgccg gaatcggact cgcgcttggc tcggcgctcg
    17461 gcctccttcc gcgccttggc gccctcaaag aacaactggg gcttggagtc aagccggctc
    17521 aggccatgcc aggccaaatc cacaatgtat gcggccagct gctccttgct cacctttggc
    17581 cggtcggccc agtactgccc ggtgaatacg gtcatgccca ccagcatctg cgcgtagtac
    17641 ggcacgccct tggcagacag cttctgccgt ttgaatgttt cggaaagaat gtcttcaacg
    17701 cgtacggcga tgtctcccat cagcgagctg aatgagccgg acgggtcggt gctcggcgag
    17761 tcgcgtacca gtacctggaa cccctcggca ttctcttcga tataggtgag gagcgcaagc
    17821 gccgtgcgtt ccacaatctg gcgcgggtgc acggtcggat cgtcgagcgc ggcggtcagc
    17881 gtaccggtca gcgcgcgcat ctcacgatcc acaatgacgg catacaggcc ctctttgccg
    17941 ccgaaatgct catacacaat cggcttggag actttggcgt gcgcggcgat ttcctccacg
    18001 cttaccgctt cgaagccttt ggcggcgaat agcgagcgtc cgacttcaat cagcttctcg
    18061 cggcgctgca gcgacgtcat acgtgaagaa tgtgccatgc ggttcagctt aagtcgccgg
    18121 gtggaattgt cggcctgaga ctgccgaatt cgtaggggat tccatctgcg ggctagactt
    18181 gacaggtatg gatatgaata ctgtgctcgc cgttcttggc gtgattgtgg cggcggttat
    18241 cgtaatcgcc ctgagcatct ggttgggcaa gtcccgcaag cgcgatttgg atcgtgcgat
    18301 gggcaaggtt gcccccgaca acaagaagac ccgcgacgcc aaggccgccg ccgacgctcg
    18361 gctggccgcc gaagccgagg aagctaaggc tgcgactgcc gccgagccgg ccaagtcagc
    18421 cgaatcggcc aaagccgagc ctgcgccagc cgcgcaggcc gaacccgaac cggcagctgc
    18481 gcccaagccc gagtcccaac ctgcctccaa gcccactccc gccaaacctg aaacccccga
    18541 atcggtcggc tctcgactga cccgactcaa ggcaaaactt gccaagtccg gcaacccgtt
    18601 cggcaaggcc ctgttcgaca tcctcgccaa agacaatctc tccgaggccg attgggaaga
    18661 cgtcgaagac acactgctgc ttgccgacgt cggtgcggat gcttccgccc aactggtcga
    18721 tgacctgaga accgacgccc gcatcactgg taaagccgac ccagccgaag tacgcgccac
    18781 gctcaaggag aagctgctcg atctggtcgg tcgagatacg gaccgccgcc tcaatgccga
    18841 aaagccgggt gccgccaagc ccagcgtcat catcatggtc ggcgtcaacg gcaccggcaa
    18901 gaccaccact gccggcaaac tggctcgact gttcgtggcc gaaaacaagc aggtcatgat
    18961 gggcgcggcc gacaccttcc gcgcggccgc cgccgaccag ctcgaaacct ggggcgcacg
    19021 cgtcaatgtg cccgtcgtcc gctccgacaa ggacggtgcc gacccggcat ccgtcgcctt
    19081 tgaggcatcc gccaaggcca aggaagcgaa cgcggacgtg ctcatcatcg atactgccgg
    19141 ccgactgcag aacaagtcga acctgatgga cgagctcggc aagattcgcc gtgttaccga
    19201 aaagaacctg ccggtggatg aagtgctgct tgtgctcgac gccaccaccg gccaaaacgg
    19261 catggcccag gccaaggtgt tcgccgaagc catcggcatt accggtgtgg tgctctccaa
    19321 gcttgacggt tccgccaagg gcggcatcgt ggtcagcgtg cagaaggagc tcggcgtgcc
    19381 ggtcaagctc gtcggtcttg gtgaaggtcc ggacgatttg gcacctttcg atcccgaagg
    19441 tttcgtggac ggcattctcg cgtagacggt cgagttcttc tcagtctcgc tttgctcgac
    19501 agctcccctc agcgagggga gccctgcgtc cgtccacgtt gtgggcgcgg gaaacgcccg
    19561 caacgcacca aacctagccc cgaagcatat tcgaaacatg atttggggct ttcccatatc
    19621 cgttggaatc atgccggaat cagcccatcc gatgccaacc taactgtcag gtgcgcttaa
    19681 caatcgcgta acttgccgta acgaaaccaa aacgagaccg gcttttactg acgaactgtt
    19741 agcccccaca agggggacgg cattgtagag acaaagtcga cgccggccgc gcaaagcccc
    19801 cggtgccctg aacgaaagag agggaggtag acatgggagc gattgattcc ggtaacaccg
    19861 catggatcct gacatccgcg tccttggttt tcctcatgac gcctggtgtg gcgttcttct
    19921 acggcggtat ggttcgtgcc aaggccgtcc tgaacatgct gatgcttgag gcggccgccc
    19981 tgtcggtcac catgattatc tggaccctgt ggggctggtc gattgcttac gccggttccg
    20041 acatcggagg catcttcggc gacccggcca gcggcttcct gctgaaggac tccgatatcg
    20101 gcgacgtgat cgaagccatc gacacgnnnn nnnnnnctcc atggtcgccc aggacggcgt
    20161 gttcaccgcc accggtctga acggcaacaa ctacccggtt tccgtggacg tcgccttcca
    20221 ggtcgctttc gcgatgatta ccgtcggcct gatctgcggc gccattgccg agcgtgtgaa
    20281 gtacagcacc tggatgattt tcgtggcgct gtgggtcacc ttcgactacg ctcctatggc
    20341 ccacatggtc tggaacggtg gtctgctgtc tgctgacggc gccatctcca aggccatcgg
    20401 cgcggccgcc cacgatttcg caggtggtac tgtcgtccac atcaacgccg cagtcgccgc
    20461 cctgatcatc gtgctcatca tcggcaagcg taagggcttc ggcacgcagc cgttccgccc
    20521 gcacaacgtg ccgttcgtga tgctcggcgc cttcctgctg tggttcggct ggttcggctt
    20581 caacgctggt tccgccttcg ccgccaacgg tactgctggt tacgcttggg tttccacctc
    20641 cgccgccacc gcagccgcaa tgctctcttg gggcttcacc gagaagattc gctccggtca
    20701 ctacaccgcc atgggtgccg cctcgggtat ggtcgccggc ctggtcgcca tcaccccggc
    20761 cgccgatgtg gtttccccgc tgtgggccat cgtgatgggt gccatcgctg gtgtcctgac
    20821 ctgcctggcc tgcggtctca agttcaagtt cggctacgat gactccctcg acgtcgtcgg
    20881 cgtgcatggc gttggtggtt tcaccggcac catcctcatc ggcttcttcg gtgaaggcac
    20941 cggtctgctg gctggtggcg actggaagca gctcgtcgtt cagctggtca tcgctctcgt
    21001 cgccatcctc tactccgcag tgatcaccgc gatcatcgcc ttcgctctgg agaagaccat
    21061 cggttggcgc gtcaccgaag cccaggaagt cggcggcatc gatcttgccg accagggcga
    21121 acgtgcttac gattttgctg gtactgccag ctccgttctc aaggaggtga agtgaaatga
    21181 agctgatcac cgctatcatt cagccccata agctcgacga tgtcaaggag gccctcgcgg
    21241 ccgccggcgt gcacggtctg accgtgtccg aggccaacgg ctacggccgc cagcgcggcc
    21301 acaccgaggt ctaccgtggc gctgaataca ctgtggacct gattccgaag attcgcgttg
    21361 aagtgctgtc cgatgacgcg gatgccgaaa cgctggtcgg tgtgatcatc aagtccgccg
    21421 gcaccggcac catcggtgac ggcaaggtgt gggtcgctcc gctggactcc gtggcccgcg
    21481 tgcgtaccgg tgagaccggt tccgccgcga tctgataaca cagacgtcca tgcctagaag
    21541 gggatggact gcataggaaa ggtccccgca cgccgcgaat tgacgccgtg cggggacttt
    21601 ttttggcaat gaaagatgaa gcaatgactt cagcagtaga tggcttgaaa caacgattca
    21661 tggatatgag ccagccggat gacgacggcg tctaccggaa tggtgcgacc aagcgcaaag
    21721 cccgcacgga actggccatg caatgcctca ctgagctgtg gaatgcggcc tgcaaggatg
    21781 tctctttccc cgtgcccgac tccggcatcg gcttcgccgc ggtaggctcg ctggcacgtg
    21841 gccaactggg ccccagctcc gacctcgacc tggtcatcat ctacgagcct cgcacgttga
    21901 acgaccagca gctcaacgag ttggccaaca aactctggta cccgctatgg gacagcggtc
    21961 tggacctcga ccactccatc cgcacgcgcg cccaatgcga ggaagtcacc gaccatgacc
    22021 ttcccgccgc catgggctgg ctggacgtga agcccatcgc cggcgacacc gctctgatca
    22081 ccaccaccgc cacgtccatc ctcgaacgct ggcgcaaggc cgcccgcaag cgcctgcccg
    22141 aactgctcga ctccgccaaa gcccgactcg acgaattcgg ccggctccaa tacgtcaatc
    22201 agcccgacat caaagaggcc cgtggggggt tgcgcgattc ggtgctcgtc tccgcgctcg
    22261 ccgcctcatg gctggccgac cgcccgcacg gcatttacga cgaagctgtg gaacgtctgc
    22321 tcgacgtacg cgactgcatc catctggtgg ccggcaagga taccaacctg atgctgacgc
    22381 cctatcaggc caaagtggct gccatgcttg gccttgccga tccgacttgg cccgaaaacg
    22441 agcgtgccgc ctactcgatc gacgatnnnn nnnnnngcac gaaggcgaat tggtgttggc
    22501 gcccggtgcc gagcctgcca aggatgccaa gctggcgttg cgtatggccg tggcggccgg
    22561 cgaattcggt cttcccatca acccgtctac cttggttaat ctgaagcgct gcccgattca
    22621 cgacaaccag tgggatgacg aatcgcgcga gctgtttatc cgtctgcttg cgtgcggatc
    22681 gaatcttatg gaagtgtggg agagcatcga tttcgtggac attccgggtc gatggatgcc
    22741 ggaatggctc ggcgtccgca accgtccgtc cgcctcggcc gcccaccggt acaccatcga
    22801 tcggcatatg gtggaggtca cgtcgcgtct gggtcgtgag acaccgtccg gtggacggta
    22861 cgatgacgat catttcaagg cattgctgtt ggccggtatc acccacgaca tcggcaagcg
    22921 cgcgttcgtg gccgatcacg ccgccgaggg tgcccgccat gtgccggtga ttctcaagcg
    22981 tatgggctac gcgccggaca tcgtcgactg ggctaccgtg ctggtgcgtg agcatttgac
    23041 gctatccgaa tttgccacgg gtaaggaccc ctatgatccg gccgtggcgg aggagttggc
    23101 cgaccgcttg caccacgaca agatgctgct ggatatgctg ttcgacctga cccgggccga
    23161 cggctcctcg ctcggtgcca ccgccggtga gaccatcacc aagcaatacg gctggtccaa
    23221 gtggcgcgaa caaatcgtcc gcggcatgta ttccgtcgcc cgcgccgcta tgtaaacgga
    23281 atcgcccagc gcttcgcgcg gccgatacct acctcatcca tgcgtccgag cgcacgcgta
    23341 gtccgttgga cagaccgcgc ccacccatga gcaccacgtt gaacgcggcc cagagtacgg
    23401 cggtacgcat caggtcgctc gtcagcgccg gcgtcaccat attggccagt atgagtatga
    23461 gcgtcacata caccacggcg gtcagcgtgc atgtgacggc cagatagcgg tagtcgcgcg
    23521 cgccgataag aatgccgtcg attgccatca tccagccttg cagtgggaag aagatgccca
    23581 tcgtcaccat gcccacggcg atcagcgtct gaatatgtgg cgtaggggag aagaagtgcc
    23641 cggcgaacag gccgactacc gcaaaagccg tgccgattac cgcgccggtg accagtcccg
    23701 cacgcccggt cgcccgcgtc agccgccgcg cctgctgcac gctgccagcg cccagtgctg
    23761 tggccaccag cgtctggccg gcgatgccca cggaatccag catgttcatc gcaaaattcc
    23821 aagaggagtt cacggcctga aacccggcca acacggcagt acccatgcgc gcggcacagg
    23881 cgaccgtagt caccatggcc gcacgaatcg ccaatgtgcg aatgaacaat ggcagtccgt
    23941 cgcccccggc tgccgcaata ccggccaaac gaggccgcag actcgcacca tccgcccggg
    24001 accacagaat ggcggggatt accaggaaca gccccatgaa ccactgagct accagcgtcg
    24061 ccactccgga gccggcaatg ccccaattca gcacaataac gaacaacaca tcaagcaccg
    24121 tgttcaccac cgcgccgccc accgcagcga tcagcgtgat acgaaccttc tgcaatcctc
    24181 ggaaaatgcc gttcgccgca tataccatca gcatgcctgg cgcacccagc acgattgcgc
    24241 gcgtataggt cacggcttgt tccaatactt cgccctgacc gcccagtgcc cggcataatg
    24301 gctcggcagc cgcgaacaat cccaatccca gtacggtgcc gatgctcaag gccagccaca
    24361 ggccgtcaat gcctgcctgc agtcccgcgc gacgacgacc cgctcccagc agatgtgcca
    24421 cctgcgcagt ggtcgagtag gccaggaata tgcatagtcc gactgccgtg aggatgatgg
    24481 tggagccgat tgacaggccg gccaacgccg catccccgat atggccgacg atggctgtgt
    24541 cgataaggat gaaggtcggt tcggcgatga gctggccgaa ggtcggcagg gccagcgcca
    24601 tgatccggcg gttggtggag cgggcgtcgg tcgggccgtt gctgacgttt gtgtttgcgc
    24661 cggcatgcgg ggtgccgaag ccatcgcggc gtgtagtatc gccggaactg gtggctttgg
    24721 cgtcggaaga gtctatgggc acgctcacac gcatatcacg cctgactgcg ttgcgcaagc
    24781 acctgttgtg ggcgcggaat gttatcccca gactatccac taagttatcc ccagtatgtg
    24841 gataacttag tggattatcc ccagaattat ccccaaaatg tggataactt atttttctat
    24901 ccacattttg ctttctgcgt gtcgcggcgc tgactcatag gtgaataagg aatgaaccga
    24961 tgcgggcaac cgtttcgttt ccatacgata tcgttcacct gccacgccac atcctatact
    25021 tggaggctat gccagaagga agtgaacact cgtatcagaa tgggttcggc ggtggcctgg
    25081 atcgtggcga tcgtggcgac cgcgcgggcc ggggcggcac gcagacgtcg gcggcggccg
    25141 gcgacccgat tttcgaccgc gtgcccccgc atgatgacga cgcggaaatg gccgtgctcg
    25201 gcggcatgct gatgagtaag gatgccatcg gcgaggtctc gcagaccatt gacgtcaccg
    25261 atttctacca gcccaagcat cagacgatct ataacgcgat catcgatctg ttttccgcca
    25321 gtcagccggt ggacgtggtg ctcgtggcca accagctgtt ggcggatggc aacctcgaca
    25381 aggtcggcgg cgcggactat ctgcacagcc ttgtcgcatc cgtaccgacc gccgctaatg
    25441 ccatgtacta cgcggagatc gtccatcagc gggccatttt gcgcaatgtg atcgccgccg
    25501 gcaccaaaat cgcccagctc ggttattccg ccgagggatc gcaggctgag gatgtggtta
    25561 accnnnnnnn nnnnnnnnnn nnnnnntccg agatctacga gatgtcgatg ggcaaggtgc
    25621 gtcaggacta tgccgccatc ggcccggtgg tccacgacgc gctcgaccag ctggacaaac
    25681 tgcagcaagg cctcgtcaac aagggcgtgc cgaccggctt ccgcgacatc gacgacgtga
    25741 cccagggcct gcagcccgga cagatggtcg tcgtcgcagg acgtccggcc atgggtaagt
    25801 ccacgctcgg catcgacttc gcccgcgccg ccgcgttgca ccacaacatg acttccgtgg
    25861 tcttcagctt ggagatgagc aaggtggagc tcgctcagcg tattatctcc gccgaaacca
    25921 atattccgat ggccgccctg cgccgcgccg acgacatcac ccccgaacgc tggaacaccc
    25981 tgaaccagtt ctggaccaag atgcagaacg cgccactgtt cattgacgac agcccgaaca
    26041 tgagcctcat ggagatccgt gcgaaatgcc gtcgtctgaa gcagaccaac gatctgaaac
    26101 tcgtagtcat cgactacctg cagctgatga cctcgggcaa ggccgtggaa tcccgccagc
    26161 aagaggtgtc cgaattctcc cgtgccctga agctcttggc caaggaactc gaagtgccgg
    26221 tcgtggccct ttcccagctg aaccgtggcc cggaaatgcg taacgacaag aagccgcagc
    26281 tctccgattt gcgtgaatcc ggctcaatcg aacaggacgc cgacgtggtg ttcctggtcc
    26341 accgccctga cttctacgac aaggaagacc gccccggcga ggccgatatc atcatggcca
    26401 agcaccgtaa cggcccgacc gaaaccttcc acctcgcctt cctcggtgcc acgtccaaat
    26461 tcaaagacat gccgcaggac tacaacgcca atcagggggt gtgatgatgg caagcaacgt
    26521 ttcacgcgat gtttcgcaag accaatcgtc cgtggtgcag acctgcaaac cgtggtatgc
    26581 gttcgccacc gtggccgcag gccgcttcgt gcggtttgca tcccgcgtca ccaaacatgg
    26641 cggttcggct ttgcccggca aagtcgtaga aaagattgac cccggcttcc tgacccgtac
    26701 gctcgggcag ttgccgcttg gcgcggtatt ggtgtccggc acgaacggca agaccaccac
    26761 cacccgcatg gtcgcctcga tgctctccga tctggggctg aaggtgttca ccaatccgac
    26821 cggctcgaac ttcgtgcgcg gcgtggtctc cgcactgctt accgaagtca cgctcggcgg
    26881 caaattggac gcggacatcg ccgtgctcga gctcgatgag gcgtacgccg tccacttcgt
    26941 caagcaggtc aagccccgtt atgcgttgct gctgaacgtg atgcgcgatc agcttgaccg
    27001 attcggtgaa atcgacacca cggccaaact gctcagccat gtggctgcgg cgactaccgg
    27061 cactgtggta ctgaaccgcg aggacccgcg tatcgccgcg ctcgccgcga aggccccggc
    27121 tggcaccacc gtgcgatact tcggcctcgc cgacgatttg cgccactatt tcccgtccga
    27181 tgacgacatg gccacgaccg tgtcggtgga aggtgtggcc gggccgctcc cctcagccag
    27241 aactcccctc agtccgcgaa gcgaactgag aggagcttcc acaggcacgg cagaacttcc
    27301 cgccgacgtg acacttaccg ccgtgggcga ccacaaggcc accttccaga tggacggtca
    27361 gggctacgcc accgacgtca agcttgaagg cgtctacaac ctgtacaacg cggccgccgc
    27421 gctcgccgtg gtacgtgcgg tgatgcaaga caatgccgcc gcatccaagg ccaccgccgc
    27481 agccaacgcc tgcgccgtgt ccgccgacga gcttatcgcc gccgtttccc gcgtcacgcc
    27541 ggctttcggc cgtggcgagg tcatcgacgt caacggttcc ccggtcgaac tgctgctggt
    27601 caagaacccg atgggcttcc gcctctcgct cgccagcttc gacccggcca acgcggacac
    27661 catgatcgcc atcaacgacg aatatgccga cggccgcgac atgagctggc tgtgggacgt
    27721 ggacttcact tccctgcgcg cgtccggtgt cgccatggtc tccggcgtgc gtgcctggga
    27781 catggcattg cgcctggggt atgaccaagt gccagtcgcc gatacgaaca ccgacctcga
    27841 acaggccgtc accgcattcg tcaacgccaa tcccggcaca cgcaagcaca tctactgcac
    27901 ctacaccgcc atgctcaaaa cccgcgccgt actgggccgc atcaccgaag tacgggacgc
    27961 gggcgtgggc aagtaac
//
LOCUS       DAWBMW010000006        27350 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_502_length_27350_cov_3.313391, whole genome shotgun sequence.
ACCESSION   DAWBMW010000006 DAWBMW010000000
VERSION     DAWBMW010000006.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 27350)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 27350)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..27350
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_502_length_27350_cov_3.313391"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         5369 a         8617 c         8189 g         5175 t
ORIGIN      
        1 attctgcccc ggtcgacagg tcaaacgatt gacatatgaa ccgggacaaa tacagatcag
       61 agagaagccc atgaagcaga tttaactttc ctcaactttc catccgccat aaactagcat
      121 tacataacat catgaaccga gacgaaacca acgaagtatc tggagtcacc gacgaccagc
      181 tcggccgaac ggccgagccg tacgaatccg gcaactggcg gggcggcgtc gggagcatca
      241 ttcgcagcaa tccgaaatca ggaaccgaca caagtcggac ccagcagaca cgcataatac
      301 gagttcacag actcggcgat gtattccgca accgcaccac cgaataccac accacggaag
      361 aactgtaagc attacgaaat caaccttgat tattgcgcgg gcctatacta ggttacggta
      421 gcgtaggtta gtggtcggga gagcgtcgcc ggccatagcg aggtgatcag tatgagcgtt
      481 tccatcagca cgccatctgg acgaaccatg gcttccgccg caacagttgt gtcttccaca
      541 accgcgagtc aaaatccagc gcaagcacca gccccaccgc actggatcgc gcatcagctc
      601 agtacagggg ccgcgcaatc gcgctatcgc caagccaagg cgcgttgaag gcgatcaagc
      661 gccagatcgt ccccgttgat gcattcggca atcgcaagcc gcgactgcgc ggatggctgc
      721 acgtcgtgct gtgcggcatc gattattcga acatcttcct catcatcgcc ggcgccgtat
      781 gctttgcgct acgcaagccg aatcccgtcc ccggctggtt cgagtttcac gaggtgtttc
      841 acttgggcac ggttatcgga tacgcctgcc acgtcgtggc gatttatctg attgcctgcg
      901 cgctgtaggt tcgctcgccc agcaccgagg cggaccttca tacgttacga gataggtcct
      961 tgaccagcca gccgtgacga ccgccttcat agacctcgtc agtgagccgg tagccgcgct
     1021 gttcgtagaa cttgtagtgg tcgttttccg agtgcacggc agccatggtt ccgccggctt
     1081 acgcgatgcg gcgttcggcg tcggacagca gccgcgagcc gatgtgcctg ccttgcgctt
     1141 cggtcagcac cgcgagccgc gcgatgacgt atcgcccggg ctggtcggga tggtcggggt
     1201 ccgcatagaa ccggcaagtc gccacggggc gattctggtc gggctggccg gactgaatag
     1261 acgaggccga ttgcgcggac aggccggggc gattgggcgc atcgttcgaa tcgaacgcca
     1321 gcagatgcac ggacacggca tcccattcat cgaattccgg ccgccagtcg cgttcgcgga
     1381 tgaacacctg ctcgcggatg gcgcgggccg cgtcgggcaa ctggtcgtat gcttcgattc
     1441 gtatggtcat gagcgccagt gtaatgcgtc acgataaccg acagagtgcg gcttgatgga
     1501 cgagttctgg cccaggtttg gcctgtgctc gatgagctca ggccgtacgc gcgatctgca
     1561 cggcggtgcc ggacgcggtg accatcagca taccgttgtc ggcaccgagc acttcatagt
     1621 cgatgtccac gccgaccacg gcgtgcgcgc ccatcgcctc cgcgcgctgc tgcatctcgg
     1681 cgatggcctc gttgcgggcg cgcatcagct cctcctcata gccctggctg cggccgccga
     1741 acatgttgcg caggcccgcg ccaagatcct tgaacatgtt cacaccggat accacttcgc
     1801 cgaagacgat gccctggtag ttggtgatgg tgtatccgtc gacggacggg gtggtggtga
     1861 cgagaatcat ggcgaggctc ctttggcttg tctgcgtaga tgaatcactg ccgagagtcg
     1921 gatcgctccc gaatctcacc tcggaaacgg atcgcttctc ttggctacca caatagtcga
     1981 gagcgccttc cccaacatct tcaatccacg cgattgcgta atcaaacaca tgtttcgcct
     2041 ccaaaaaatg ctgatagaca ttgggccgca aaaggccagc ctcaacgggc gcaagccgct
     2101 caccaaactg aatcgcagac ccaaatgact cgtcatgggc aaacgggtgg accacccaaa
     2161 ttccgctgcg ataatcaacc atcaccgaaa gcccaggctg ctccgtcgat acgaccatca
     2221 tcgaaccgga cacgttctcg acattgatat tcgggtgccc gtacaacgcg taactcaaac
     2281 catcatcggc aacaccctgc tcttgccaat tggcgcctaa gcactccaac atctcgctgg
     2341 gcgagtacgc acaatcttcc gcataatcaa tcggctgagc atccgtataa cagcattcac
     2401 cgccgtcacc ttccgcaatg ctgcgctcca caagataggc catcacatcc ggcatctccc
     2461 aataatcaat ggacagctta tcaatacagc cgtttttcca agccatataa tgcgtttgct
     2521 cgtcgccaag aatctcttca aacgcctcgg ctgagataaa cgtcgacccg tccaactgat
     2581 agcactcgtc caaaggatag atttctcggt tcgcgtattg ttcctgagcc ttggacacaa
     2641 gcttgtcatc caatgcgctc atctgctgat caacttcatc ctgtgaggga atgaaagggg
     2701 cgaagtccag ccctgcttcg ccacagcctc tctgccgttc acgcctgtac atcacgtccg
     2761 cacacgtatc ggtctgccgt tcattcacca ttgatcagcc tttcttttcg ttccacaaat
     2821 gccctagaac actgcgattc cagtagttcc gattgaatca ggatcgttct tgcgaaactg
     2881 tcgaggatcg tggatcgctt gtggatcttg aagcgttgta tcctggttgt tcatggatcc
     2941 ggaacgcaaa atcattaacg tcgtgggagc cgcaatcgtc accaacggca ccgtactatg
     3001 tgcacaacgc ggtccgggga agtcgttggc cggatactgg gaattccccg gcggcaaaat
     3061 cgaacctcat gaaacagcgc ggcaggcttt acaccgcgaa atcgaagaag agctgttatg
     3121 cgaagttgaa gtcgcggaag aagtgtgcac cagcgaatac gcctacgact ttgggaccgt
     3181 acggctcact acattcgtct gtcacctcat cgccggcaca ccgcatctca ccgagcatac
     3241 tgatatccga tggctgaatc ctgccgatat gccgacattg aactgggctc cggtagatcg
     3301 cgaagcagtc gcgattatcg ccagcaaaca gctgacgctc cgatgaatgc gccatagcgc
     3361 agccatgcag tcatgcagtc atcaacaaac aattcgaatc gcaagggaac aagacaacat
     3421 gtctatcaat ctggccagca cctcaagctc tccggccaac acgcaacata gtgatcctgg
     3481 cccgacaccc tcaatcctcg aagatgtcat ctcaggtctg atcgagccgc gctcagatgc
     3541 tcccggcgaa tataagccga ccctcatcgc aaatcgcccc ggtggattga ccatgcgcga
     3601 ggaaatcggc aattccttgg ctactagcga ttccttcgac atttctgtcg cattcgtctc
     3661 ggctgaggcc gtgctcagct tatttgaaga cttcagaagt caccatgaaa cagcgacccg
     3721 agcgggcaca ctgattacct caacgaaaaa tcatttcaac gatcccaggg cattctggga
     3781 gctgctgcat cttcagaaca ccactggtgt tgacgttcgc gtgtgggaag gctcccgcaa
     3841 tgcctcagtg gccgcaatgg ctcaaggaca gccattccat ccaaagggat atatcttttc
     3901 tcgccgcatg aaagacggta cgccctatta cgacctgtac gttggcagtt ccaatctcac
     3961 tggagccgca ttgaccacgc aacgcgaatg gaatctcaaa gtctcatcgc tggcggatgg
     4021 agagctggtc gggcagttcc aggatgaaat cgactcacag gtggccgatt ccgttccgct
     4081 gaccgaagag tggatcaaac agtatgagga agatttcaag aaatacgccc caccgcgtca
     4141 tgagattctg cagtcgtttg aggggcatga cattcagcca aacgccatgc aacaggaggc
     4201 gttggcgaat ctcaagaaac ttcgtgaaca aggcgagcac cgcgcaatta tcgtgtcggc
     4261 caccggcacc ggaaagacgt acctttccgc attcgacgta cgcgaatgcc agccgaagcg
     4321 catgctgtac atcgcccaac agcagatgat tcttcaaacc gcaatgaact cttaccaaaa
     4381 ggtgctcggc tgtgatgaat ccgaattggg attgtattca ggcaccagca agcaacagga
     4441 ccgccgctac gtattcgcca cggtacagac gatgcggcag ccggaggttt tggcgcaatt
     4501 caaatccgat gaattcgact acgttctggt tgacgaagtt catcacgctg gagccgaagg
     4561 atatcagcgc gtcatcaatc atttcaaaga tgccgacttc atgctcggca tgaccgccac
     4621 gccagagcgc accgacggca tcaacatctt cgagctattc gggcataaca tcgcgtacga
     4681 aattcgcttg caaaaggcgt tggacgagaa catgctctgt ccattccact actacggcgt
     4741 tgcggaatac cttggctccg acgacgatcc gaatggaatc gcccatcgtc tcgatgtctc
     4801 caagggtctt gatgcgaagg actcgaaaca gctcaagtac gagatcgagc agcttgccac
     4861 cgaaaagcga gtccgctaca tcattgacaa gttgcaggaa tatgggcagt tcaacattcc
     4921 ggtcaccgga ctcgtgttct gcagccgaca ggaggaagcc cataagctgt cccagctgtt
     4981 taaccagcag tggaatcaac aagatgaacg cccctatcgc accgctgccg ttaccagcac
     5041 ggacgatgac ggcaggcccg tgtcccaagc gcagcgagac gaatacgtcc gcaagctcac
     5101 tgaaggcgaa ctcgactatc tcttcactgt cgatatgttc aatgagggag tcgatattcc
     5161 cgccgtcaac caaatcgtca tgttgcgcag caccgaatcc agcattatct tcacgcagca
     5221 gctcgggcgc ggactgcgca aattccctca caaagaaagc gttgtggtta tcgatttcat
     5281 cggcaattac aacaacaact atctgattcc ggtggcgtta tacggcaaca ctggcgaccg
     5341 cgacagggct cgcaagaacc tgcagcgaaa gtcaatcggc ctgtcgtcca tcagtttcga
     5401 cccgatcgcc aaggagcgca tccttaagtc tttggacacc gccgactggt ccgacatgaa
     5461 aaagctgtcc gaacagtatc gccaagtccg ttacgagctg ggccgcatcc ccatgcttac
     5521 ggacatctat aactacgacc cctcactgcc gtataccatc gcatcgaagc gcagcaacta
     5581 tcttgatttc gtgcgttccc gtgaaaaaag cctgggcaaa ggcaaacatc atgaagcgac
     5641 atttgaggat cagttggagc cggtgaccga cgttgaagac gcgatactca agatggcagc
     5701 ggagctgctg ctgcccggat tgcgaccgca cgaattggtc attcttgctc agctatgcca
     5761 tttcgtcagc gaacggctcg acgacgacgt acctcagcat tggagtgcgg gctcgtcaat
     5821 cggccgcagc gaactgctcg atgcgattcg tactgaattc ccgctggccg atggttcaga
     5881 cgcgcaattc gactctgcga tcagcgtgct cgattattca tatttcaccg gcccgaactg
     5941 caatcgtttt ggcaaccagc cattggtcga aaccttgaac agcaccggca caggaagcga
     6001 caccgcatat cggttgagca gcagattcgc cgacatactt gccacgaacc gaacgttccg
     6061 catcttcttc gcggacactt tgcgcaccgg tatggccaat tgccgagata tgttccgcga
     6121 ggccgccgcc aggcaacagg tcttcgacca catgttcttg tacgagcgca aatattccat
     6181 ggctgacgtc atgcgcctgt gcggatggaa gaaggaaaac actccgcaaa acgtgggtgg
     6241 ttacctgctc gacaaggaaa ccaacaccat gcccatcttc gtgaaatacg cggccagcca
     6301 atacgaggat gaattcctga gcacacagga aatgaaatac ttcagcaaga acggacgcac
     6361 tccgcagtct ccggaattcc gatgggcgct tagcggcatt ggcccgaatt gggaccacac
     6421 acatttcgtc ccgctattcg tcatgcgcaa ggcggaagag gccgatggca agtactacta
     6481 cgttggccac gtcgctgcgt ttgagaatcc tcagctcacc accaagcctg acgcaaccgg
     6541 acagggcagt gtgaaggtca ccctgtcggt cctgcgtttg gcccggccaa tcgatccaga
     6601 gctctatcgg catttagccg gctgaccatc agctcctcaa ccgtacggcg gcggccattc
     6661 cgccgtcgcc gtaggtgggg tgggccatga aggcttgact ggcggtggcg tcgccgcgca
     6721 tcgactcgtc gtagccgcca cagcgctccc acgcggcacg gcgaattatg acgtggccgg
     6781 gcagcaatta tgcgacacga acgtggcggc gatgccacgg ttacgcgcgg cgcatcatgc
     6841 gatgctcgcc ggcgaccgtg tgctcatcct cacgcttggc gggcttgcga gcgcccgcaa
     6901 tattctccat gctcatgcct gctcatcccc atccacaacc gaaatccagt cgccgtatcc
     6961 ttcctcggcc atgcgcgagc gcggcacgaa gcgcagggcg gcggaattca tgcagtagcg
     7021 caggccgccg cggtcggccg ggccgtcggt gaacacgtgg cccaagtgaa tctgtgtgtc
     7081 cgacgtgcgc acttcgatgc ggtcgcggcc cggaatgcgg tggtcctcat gctcggtcag
     7141 caagtcaccg gcaatcgggc gagagaacgc gggccagccg cagccggaat cgaacttgtc
     7201 tcgcgacgag aacagcggct cgccgctgac gatgtccacg taaatgccgg gctcaaactc
     7261 ctcgtcatac tcgttgacga acgggcgctc ggtagcggcg ttctgtgtga ccgcgaactg
     7321 ctcaagcgtc agatcccaga tgcgttccac gtacttctga cggcgtttta cgttcgcgat
     7381 tgcggcgatc ggcacatggc agtagccgcc gggattgttc accagatagt cctgatgatg
     7441 tgcctcggcc gggtagaagt tgcgcagcgg ttcgaccagt acggccgggc gctgcggctg
     7501 gcggtcgacc agctgctcca aggccgccac atacacggcg cgctgcgtct cgtccgtgta
     7561 gaacatgcca gtgcggtact gccggccgcg gtcttcgccc tgctggtcca cggagaacgg
     7621 gtcaatgacc tccaggaaca gtaatgtcag cgtccgcagg ctgacctgtg ccgggttaaa
     7681 ctcgactttc acggtttcgg ccgcatccgt gccgcccgcg catacctgct cgtaggtcgg
     7741 cgattcgacg gtggattgcg cgtagccgac cgtggtgtcc gtgacgccgt cgacgttctg
     7801 gaagtagcgc tccagtcccc agaagcagcc gccggcgaag tatgcggttt gcgtatttcc
     7861 gtcattgtgt tcgttacgtt cgctcatgcg ccccacgtta ctacgtcgag gcggctcgcg
     7921 atgcagtcaa cgacacctct gcaccggcaa aaccgctttc gaggggctga ttcggccttc
     7981 gcaggaggtc tactcccgtt tcaaggggct ggtcggcagc tagaccgcat gtaaaagccc
     8041 atccgcgtca acgccattcc gccgattttg gagcgtgaat acttcggcat cagcccctcg
     8101 aaacgggagt agacctccgg gagagaccaa atcagcccct cgaaacggag atagcgcttg
     8161 ccgcgccctc cttgtcaggc tttctccgtt caaagaggta cattcgggct tctgcagaat
     8221 gctatcttcg ttcaaagagg caccgaatac gggaaacccc gagtacaaac gttgtcattg
     8281 caacgctcat actcgggatt tcagagttca ggtacctctt tgaaggcagt tagttgtccg
     8341 caagaggcca aatgtacctc tctgaaagca gataccccgt ctcaggctcg aatcggctag
     8401 cctgcatacc cgtgtgcaca ccttcacgac cgcagcgtac tgagcatgcc ggccacttcg
     8461 gcgggcgtca cgctgggcac gtaggcgcgc accaccgcga gcccgatctg cggcaacgcc
     8521 gccgggtccg ggtagcacac gtagaccggc gcttcggcgg gctggaactt gcgcttgaag
     8581 ttgaacagcg agtggaagcc gtaggcgggt tccatccagt cggccacgat ctgcagcgcg
     8641 tgttggagca cctgcgtgcc ttcgccggcc gccaccccac cttcccgggc attgtctcgc
     8701 tcggggttca tgccggccaa gggtgccgcg gacaggctca tgaactccac ggcatgctca
     8761 ggatccgcaa gcccctcgtc gcgcaggcgc tcggccatgc gcgcgatcag gaactccata
     8821 atgccgttgg gactgtcggt gcgatggcgc atgaagtcca gcgtccagcc gacgatgcgc
     8881 ccgtcgcgcc aggtgggcag ccagctggtg acgcccagca cgcgcccgtc cgcgtcgatg
     8941 gcgtaaagca gccgcacgcg cgggtcgcgc agttcctcga caccgccgag cgtgaacttc
     9001 atttccggca gcgccttgag ctgcgcccac tcctcggaga tgtcctcgat ctgctcgcgc
     9061 acatcgagcg acgcttccag gaacgtggac tgcacatcgg tcacgccgtc gcgcttggcc
     9121 ttgttgatgg cggtgcgcac atcctgccat ttcttgccgg tcgtcttcca gccgcgcggg
     9181 tccacgacca tttcgctgcc gacctcgatg gagctccagc cggcctccag caacgcgtcg
     9241 cgctgttcgc gatgcacgct gtagaacacc ggcgaaagcg accgctccac gcaatactgg
     9301 gtgaagccgg tcaggtcgtc catccactcg gagggctcgc cgaacggccc ggtgcaggtc
     9361 aaggcgatgc cgttgagcac gcgatatgcc acggccgatt tgcccgtggg cgagagccag
     9421 tagctgttgc cttcccacgt agtcatgaag ctcatcgatt cgccgccggt ttcgaccagc
     9481 cgctcggcgc gtgcctgcgc ccgctcgttc gattcgctca cgtcgctcat ccagcggatc
     9541 accacgacca gcaccacgat ccagaacacc aggccgacgc cctgatacac gatcgaggcc
     9601 atcggggtac gcggcacgaa cgacaacttc atatggctca ggaagccgat gggcaggaag
     9661 cgccccggca gttcggccaa cagcgacgag gccgtggcac gcggcgagaa ttcatccggc
     9721 tgggcgatgc cgtacatcag gtacacggcg gcgcacgcca gcagaaccag cacgatggca
     9781 ccgacgccgc cgatcagccg gcgccagccg acgcgaatcg ggaagtgctt cattgccgca
     9841 gccaaggcga cggcaaagaa cagcggcggc aaggcgttcg ccacgcaggc ggtgatcgcg
     9901 ccgtgctgca gcagtgaggt catgccgtcc ggcgcaaagc tcagcggcac gatcaggtag
     9961 taggcgatgg ctactcccgc agtgaacagg ttgatggcca cggcacacat ggccgcgaac
    10021 cgacggccgc gatacaggcc ccacgccagc accagagtca cggcggtggg cagcagcgag
    10081 cgcagtaccg cgccgggcat cgaggcgcgc atcaggtcga attgcaggaa gcagccgctg
    10141 tgcgtggcgc cggccaggca gcgcgcgagc gtgccgtcgt cgacggagac cggactcatg
    10201 agcaggccga ctgtgctgag cggccccgcg tgattatgcg aggtgatggc gatgaccggc
    10261 cccagcgcca gcaccacggc gatggccgcg aacatgcgcc gcgcctcgaa ggaggtgctg
    10321 cgcagccagt gccatccggt ttcggcgtgc gcgggcgacc cggccatcac gcggccgacc
    10381 gcgtgtccga tcagcgcggc ggcgagaatg cagtaatcgc caggattgcc gctgtaaagc
    10441 agcgcgacca gaatcgccac gtagccgatc agccgaatac gccgccgcaa cagatggctg
    10501 cagaacgcgc tggccgccat aagcgcgccg accggcaaca ccagcgggct cagggcgaac
    10561 tgcatgcggg agacgaattg ggagtcctgg aacagccatc cggccgccgc acacaagatc
    10621 aggcccacga tcacgccgcc gagcgcgcag gcgagcgcgg tgccgacggt acgtgcgcgg
    10681 ccaagccacg gctcggcgat tgacagcatg acgagccaca gcacggcatc aagaatgagc
    10741 tggatgacgc cgcgcgccaa gaacagcgtg cagaagagtt tgccgaaatc gaattcggcc
    10801 atgctggtgt cgagccgcag cgggaacgcg aatccggcca tcgcgaccac cagccacacc
    10861 accaagttca gcaccaccag cgccaccgtg gcagtgatgg ccagtcgatg accgtccgcc
    10921 cactgtcgca cgtcgccggc cagttcgcgc cagctttccg ccgcatgact gttgtgctta
    10981 cgcgattctt cgctcattgc aacacctcca cttgtgggta ctccttcaac gatttcgcca
    11041 tctcgcccaa gccggtccgt tcgccaaacc agtcgagacc gggccgccag accgcctgca
    11101 ccgcgtgcca gtcgtggccg gcgccgggca cgatcagctc ggtgacctcc atgccggctt
    11161 tgcgcgcggc ggcggcaatc gtgcgcatgt tgctaatcga ttccttgtcc cgttcgccag
    11221 caccggtgaa cagcgcctgc tcgggcgtac cggtggcggc gatggcgttg accggcacct
    11281 gttcgtcgta cgccttgcgg tcgccggcga aatactcctg gaccatcttg gcgacgctgc
    11341 cattggtcgg cttgagttcg ccgtccaccg gcagcatcgc gccgtagatg tcggggtggc
    11401 gcgggacgag ctgcgtggtg caggtgccgc cttgcgagaa cccgcccatc gcccacatgc
    11461 gggccgattt ggccacgggc agcatcttct tggcccagtt caccacgtct gtggtgaggt
    11521 aggtttcggc gttgccttgc gtggtgtcgg cgcacaggct gttgtgcgtg gcctcgccgt
    11581 tctggtcggg cacgagcacg atcggcgcga gcccgtcatg tttggcggcg tacgcattca
    11641 tcgtggcggc gatgttgccg gcgtcgatga ggcgactggg gctaccgggc tgcccggcca
    11701 acagctccat caccggcaag gccggcggac ggtcggacag cgcggcaggc ggcaggtaga
    11761 tcatcgcctt gcgggccgtg aagttggatt cggtgttcgg aatgtcgacg gtgagcacct
    11821 tgccttggct gggcatggac ggcaggctgc ccttccgcgc cttgctatgc cagtcgctga
    11881 cactcatcgc ggccttgtgt acctcgattg agccgagcga ggcgtacggc gtgtagccga
    11941 cgagggtgcc gatggtctgg tattcgccgt aaatcgagtc cacgccgagc gcggtggaca
    12001 gcagcaccag cggcaccagc acgacggcca gcgcacggcg gaccccgcgc agggtcacgg
    12061 ctgcggcgat cacgaagccg acgccggcga tgccgcccgc gatggtgaag atcaccggcc
    12121 agcccagcga tacgccgaac accataaaca cgtcggacac cagccatgca accagcagtc
    12181 cagccgcgcc ggcgagcacg gcggcaatca gcgagacaat cagcgggtgc aggcggccgc
    12241 gcttcttgct cttgctcaat gccagcccaa taatcagtat gattgccgcg atcgcggtca
    12301 gtccgaacac ggtccgcggc acgattccgc ggaccagaga caattccatg aacgttttcc
    12361 aaacggagtt cttcatcctt cacccccgcg ctccaggctc ctcgccttgt aagctcacgc
    12421 aacactagcg cgcgattcat agagcgtcac ccaatttcac aaaaattgtc gcgagttctc
    12481 atggtttccc cagactccgt tcagcgcctg ttcacatccg gtctggcgcc cgcagttcaa
    12541 ggccagtgtc ggcgctggat tagcggcgcg caggcttgtc ccagcttcag ccggaagcag
    12601 ggaatatgct ggtggatatg actcacgcaa acgaacaacg tccagcagcg accggcaatg
    12661 ccgccgataa cgtgtctgac gcccctactg gccccgcgac gagcgcggcg gcaaggccca
    12721 cgaccgtcac ggctgatggc acaacagcca caaacgccgc ggccagttca acagcaaagc
    12781 ccgcagccag cacctcttgg gccagggaaa ccaacccacg tcgagcactg ttgacgatta
    12841 tcgtcacggt ggtggttgtg gtggccatcg tactggggct cggattcttc gtgaactggg
    12901 gattggcggc ccttttggtg atcattatga ttccgggggc gatttcgctg gccgttctcg
    12961 ccaagcaccc tgaatccacc ggcgggcgca gcatgttcgg catctccgat ccgaatacgc
    13021 tgcttgaccc gattcgccgc ccgtccgagc gtcgcggcga gtaagttgct gctgcccatc
    13081 gggcggtaat ttatccatgg ggcggtaaaa ggcccatggg gcggtttgaa gaaattgtga
    13141 aaccgcagaa taaagtcatt ttgggatacc gccccatgag ggttttaccg ccccatggga
    13201 ttattaccgc cccatgagca aagccacccc gctatttgcg cgcactgacc aagtaattaa
    13261 gcaccgatcc gacggccacc agcagcgcgc cacaccaaaa cggcagcgac agcgctaaac
    13321 ccagcaacac cgcactggcc ccggtggaca gcaccggcgt ggcataggac gcaatagcca
    13381 gccgctccat agacccgtgc agaatgccat agccccagca ggcataacca ccgccaatcg
    13441 ccgccgcggc aatcaacaca aacagccatg cgaccagact cggcggctcg gccggccacc
    13501 cctgcccgga cgcgaagtgg ataatccaca acgccacggc cacgcagggg aagaacaatg
    13561 aagtgccgtc caccccatga ctcatggctg gcgtgaacac cgcgtacacc gaccaagcga
    13621 gcgcgcccac gaacgccagc gcatagggca aagggttggc ggcgatatgc tgcacggccg
    13681 catgccagtc gagcccggaa ttaccgccga ccgccaacac cacgccggca gtggccacga
    13741 tcgcgcccgg cagcaccttg acgaccgcgt gcttgcgatg ggatacgccc gccgacagca
    13801 gcaccatcat cgtgggccac agatagttga ccaggctcac ttccaccgag gatgcgtcgg
    13861 tagaggccag acccagcgag agggatatcg acgattcgta aaatacgaac aacaggccgc
    13921 cgataatcag gtatttcttg gggaactcgc gaatcggggc cgggcgccgg aacatcagca
    13981 gcaggatgcc gccgaccgtg tagatcaggg ccgagccaag cgtggcgccg aagttttcgg
    14041 agacgatgcg cacggtgccg gccatgaagc tccacagcag gattgccatc aggccgatgg
    14101 tcgtggcgcg cgcggatacg acgtggccgc cggaatggct tgccgccggt gccggtgtgc
    14161 cgcttgcgtt ctgtgcgccg ttcgccatat gtccgcctct ctcgcatcgt ttcgaagtca
    14221 ctctacccat cggatgtgta cagcggcaga acggcacgcg accggcgcgc gaacagggtg
    14281 gggttcggtg attgaaacag caaaatgccg ggtagggtaa atccctaccc ggcatttgca
    14341 ttggctatgt ttcccgccgt ctgtcaggtt agtcggcgtt gaaagcgtag ggcagacggc
    14401 gtgggaagca tggtttggtt acgatttatc tctcccccac ccgcttcgcg ggagccccct
    14461 cgtcagaggg ggcaaagcta ctcgaagcgg gacttcggga cgaatcaagt atcgtcagcc
    14521 ctcaatcgcg atttggtgct tggactcgac ttgcggctgg gcctgcatct tcggaacctg
    14581 gatgcacagc gtgccgttcc ggtagctggc gtggatgtcg gactccttca cgtcctcgcc
    14641 aacgtagaag ctacgcgagc aggagcccat gtagcgttcg cgacgcagcc acttgcccga
    14701 atcggacgcg caagcacact tgctgtcgga agcctcgtcc tcagcggccg gagccttgtc
    14761 ctcatgctcg ctgcaacgcg aggcggacac ggtcagatag ccgttgttga gctcaaggtt
    14821 gatatcttcc ttcttaaagc caggcatatc gatgtcgacg tcgtaaccct tgtcggtctc
    14881 gcgcacatcg gtagtcatca tgttggcggg cattacggca ctcatcgtgt tgtccgtgtt
    14941 gcgccaaccc tcaaagaacg gatcatcgaa cagatccgag aacatcgtgt cattcatcaa
    15001 agccggaaac attgccataa tcggtactcc ttgattagga gagaatcggc ttggctgccg
    15061 gccgccaacc cgtatcgcgg ggctagcagc ggttatcgtg gccggttccg gaaccctccc
    15121 gggctctcaa cctttaacct tcacttcatc caattcccga gcgcggcaat cattccggga
    15181 ttatgcccac agagaaattg cggcgggaat ggctgccggt aaagccggcc gagccgagcg
    15241 ccgtgaccac cctcagccag tcttacggct gccagctccc gccagcggga gcatatatac
    15301 gcacaccagt aggcgtactc atatacctct gccaacggga gcatggttgc acgttcccgc
    15361 aaactccgcc aacgggagca tgctggcgcg cgaacggctc acatctgctg ggcgttttcg
    15421 agcaaggcgt ttttgaggtc ggactcgatt tggcgcatct tgacctgggc ggcctcgcgg
    15481 ttggcctcgc cttccttctg gatctgcacg gtctccttca gggtggcgat gagttcggcg
    15541 ttgatcttct ccagcgtgtc gatttccacc acggaacgct ggttggcctt ttccgcgtcc
    15601 actgcaccct tgtgcagggc ggccgcgtta cgcgccagca gcttgttggt cacatcgtcg
    15661 gccttgttct gcaggtcgag aaccttgcgc tggttggaca ggcccagcgc gatcaccatc
    15721 tgactcttcc acaccggaat cgtggtggag atggtggtgt cgatcttgtc cacgatggtc
    15781 tggtccgcgt tctggatgat tttgatctgc ggcgcggtct gcaaggtaac cacgctgatg
    15841 cggtcgaggt cgtcgagtcg cttggcaaaa cggtcgagct tggacttgaa gttcttcagc
    15901 acttgcgcct gcatggcgtc gccactggcg gcggcctcgg cctccagcgc tggcagctgg
    15961 ttggcgttga agtcggcgag cgccttcttg ccggccagta cggtcatttt cagctcgcgg
    16021 tattgctggg cgttctgcgt gtacatcgtg tcgtacatgg agatgtcggc cacgagtttg
    16081 gcttgcgcgg attggagggt ggtgatgagg tgttgcatgg catcgttgtt gggcagttcg
    16141 gatacggccg ggatctggtc gagcgtgccg gccacgccga atcggctgat cgaatcggtg
    16201 ccggcgaagt tggccgcgcg gaagccatgg cgctcccacg ccagcttctg caccgccggt
    16261 gttttcaaca ggttcagcag tgtgccgcct tttctcgtcc aaggccatca gcgtgtgggt
    16321 gctccacacg gtcggcgtcg ggtagacgat gaccacgtcg tccttgatct gcttgaacgc
    16381 atccggctgg ttcacggcca aatcaagcag ctgcgattcg tagcccacca tcatcggctt
    16441 ggagcccacg cccaaggtca ggaactggtt gaacgaatcc tcggaagagg tctccatcca
    16501 gccggatttg gcgaagatgg aggcgatggt cttgccgtcg cgcgccaccg aatcgaccat
    16561 ggccggctgc ccgccgttga gcaccgtggc aagaagcgcc gcgtattcgt tgcctgagtt
    16621 cgacttcacc ggatcggtcg agtcgatgcg gaactgcccg tagcctgccg tgtatccgac
    16681 gtcggccatg ttgtgttggc gaccatcgcg tccacggctt tggccatatc catgtggtac
    16741 gcgccagaat cgtccttggt cacaagtccg ccgttcacga gcccgtccgc cacggccttg
    16801 tgcgtgtata gcacaatcgg tgagttgaag acgatgtcgc tttggggggg ccttgacgcc
    16861 tttcgcattg ccgtattcga cggccgcgcg cgaggagggg aacaggtaac ccatgccttt
    16921 ccggtccgta tccatcatgg ccggcgatcc ggccttgcga tagtcgatgg caatgccttg
    16981 cttggcggcg agcgccttga acttggcgtc atcgaacagg ccgattttct cgccgcccag
    17041 gtagccggtg atgctcacct gttcggtcgg ttcggccggt gctggttgcg attgccgcca
    17101 caccagcgtg ccgcagacca cgcccactac cgcaagcagg gcgatcagtc cgatgatttt
    17161 tgatttggag agtttcattg tgcgtccgtt ttctggtccg actggtgagc cgggccgcca
    17221 gatggagtct gactgggtgc atgccgtcct tcatcagcag ccgaggaagg cacaggcgaa
    17281 ggtgcgacgt cgtcgtatcc atccatgcgg gccaaccgtt ggatggcgtc ggagtcggcc
    17341 gtaatgccca acgtcttggc gcttaccgcc cggctgagtt cgccggcggc ggtctgttcg
    17401 agcacttgca gcgcctcgat ggtttcgatt cgtgccgtgg ctatctgatc gcgtgcgccg
    17461 gaactttcca cgtccacgta ggagcgcaac agtttctcgg tctgctcgcc gtacacggtg
    17521 atgtagtggc gcaacgtcgg gtaggcggag gtgtctcggg tcacgtattg cactaaatct
    17581 ttggtagcgg cgatgaagtc gttggcttcg gctttgacgg ctgggtcacg tacttgcttg
    17641 gtgagcatgg cgatcgagtt gagtcgagcg ttcgccgcgt cgatcgcatc ggcggtcttc
    17701 tgaccgttgg gcaaggcgga aaccagcatt ttgccgatgc gtcgctccgg ctcggtgagc
    17761 gtggaggcgg cgatataccc aagtgcggcc agtaccacgg ccacgatcag gccgggcact
    17821 ccgccgccga ccagtccgaa accgagcccg gcgagcagca gtccgacaac gaatccacag
    17881 attacctgga gcatcagttg aagcccttgg cttggcggaa gacggcggcg aggtcgccgg
    17941 agcggccgtc gaataccttg gcgttgctca gggtggcgag agacttcagc tggctgggat
    18001 cggcatcgcc gaacatgatg gagaagatcg gcagatcacg gccgcgcgac ttataggcgc
    18061 tttcgaattc gtcctgatga tcggagttgg agcggccgtc ggtcatgagc acgatggcgg
    18121 tggtgtattg cgaggcttcc gactcgctgg gcagctcgtc gagcgcgctc agcaggcctt
    18181 catagatatc ggtgccgccg ctggcatcgg tggcatcggc ctcatgcagc aggtcgctgg
    18241 tgcttgtgcc agtggccttg accgggcggt gggcctcggt ttcaaacggg atgagaatat
    18301 tcacgtcgcc gctggcgggc tcgatgtacg acttcttggc ctggtccggg tcgagcgccg
    18361 cgttcaggcc tttgaccacg ccgttcttgc cctctccaga catcgaaccg gagtaatcga
    18421 ccacccaaat ggtccagctg ggtttcctga gcgcggtctg gtagacgttg agcgcgctgt
    18481 cgatcaccgt ggcggaggtc atggagaacg tgagtttgcc gtcgctgacc gcggcgagga
    18541 tgtccgcggt ggagacgggc ttggtggcgc cggtgtcgtc agcccagccg agtttgacgg
    18601 ccttggattt ggcgatgccg aacacgatcg gcgtggtgga ggtgctggcc gcgtctttga
    18661 cgatgtgctt ggtgtctccc atcgaaatcc acatcgagga ggcaggccag acggcgtcgt
    18721 gatcctggcc gccggccttg agcgcgttca taatctccag cgaacccatg tagtgcatcg
    18781 tcacggctac gttggattgg tcggccgctt tctggatggc gacggcgact tccttgtttt
    18841 cggagccggg ggcaatgctc agggtggcag tcggtttgcc gccggacggc tggaaggtct
    18901 gcgtggtagc ggacgtgccg ttgccgtccg ctgtgccgtc atcggaatta ttgaacgccg
    18961 tgcaaccggt caacgcgagc acgccggcac ataccgcggc cgcaaccgcg gcggcggaga
    19021 gaagacatta tgcccataat tccatggctc tcccgccatc taaagcgggt cgggccacaa
    19081 ggtgaccgca cggtgaacaa tgggtgaaga gctacatcag tacgcggcgc aggcaatcat
    19141 ggggtcgtcg tagaagccga ggatcttttc gaggtagggc ttgtaggtgc tgttgggcat
    19201 ggagaagatt tcgtggcgcg attcggggaa gcgcacgatg ttcgcctcgc cgccgccgtc
    19261 cttgaccagt tggacgaagt gattctgcgg cttattgagt acccagatgt cgcgtccgga
    19321 ctggaagagc agcaccggcg tctccacttc ggcgcaggcg gacggattca gtatggcccg
    19381 gttgagcttc aacgcttggc gcacccattc gaacgccgcg cagtaggtct ggtattcgct
    19441 attctcgcaa cgcagcttga agtaccagcg ttcacgcgct tcggaagcgc cctcgttgcc
    19501 ttccatcgag aattccggcg tgaagcccga ctgcccgaac acgcgcttct tgccgaagcc
    19561 gagtccgcac agcgcgccga ccagcacacg ggccacgccc agcggcatgc cggtggcggg
    19621 ggcgatcatc ggggcagaga gcaccgcttt gtcgaacagg gtggggtatc gctccaacac
    19681 ggccgcgccg atgccgccgc ccatcgaatg gcagaacagg ttcagcggca tgccggccgc
    19741 gtactgctgg ccgatggttt cggcgaagcc ggccagatcg gccacgtagc gctgccagtc
    19801 atcaatccac accatgcagc ggttgtccac gtcgcgcgcg gacttgccgt ggccgcgatg
    19861 ctcgagtaca cacaccgagt agccggcaag caggaagtac cagacgagct cgtcgtactt
    19921 ttcggcgaac tcggtgaagc cgtggctgat gacgatggcc ccgcggaagg tggcggtggc
    19981 gccttgctcg cgaatcgcgt cgaatttggc ggagtcgtag cacaggtagt gcagctggcc
    20041 cgagcggccg ccggtgtcca taatgccgga cagcggctcg atcccggccg aacgttcggc
    20101 ggcggacgga tcgaaccagc cctcctcgca gcattgttcg agcgcgggca gcaccacgtc
    20161 ggtcatcgtt tcggcgtact tgttttcgtc gatcatcgtc acctgcatgg ctcccatctt
    20221 aggacaagcc tcacgcccac acctcgcacc cacaccacga cacattcccg ggaatgcgtc
    20281 gcaagcctgc gcgccactga ccgcacaggc gcgtaggttg tgaggtatga gcgaaaccaa
    20341 tgatgtcaat gacgcaatca agcatttccc acgcctccgg gcgcgcacgc tgcgcttcgg
    20401 ctgcggcgca ccgcgctccg cacagaccgt gggcgacggc tcgcgggccc tgttcctgcg
    20461 ctccgacggc cccgaggatc tggtgaccgc actgtggctg agctggttcg acgagtccgg
    20521 cgaacaccat gagaccaagc tggccgaccc gcgcgaactg ctgggcgcaa ccgccgacag
    20581 cgaggacgtg ccggccgagg agaaggcccg ccgcgagcgt gcgcgcgaag gcggcaccgg
    20641 catcgtcgga tactccgcag atgacgacgg caatcgcatc gtgttcacca tcaacggccg
    20701 cctgttcctg accgacatcg attggaatga cgagaccggc gcacccgagc cgcacacccg
    20761 tgaactggcc ggcgaatggc tcgatgagga cccggcgatg tacacgccgg tgctgaaccc
    20821 gcgcatcgcc ccggatggcg aacatgtgct gtacaccacg ggcagctacc tgatgctggt
    20881 cgatatcggc ggcgaactag gtgaccgcat caccgccgtc tacggcgtga gcgtggagga
    20941 tgaggacggc aatccggccg aaaacacgtg gaaaatcggt ctggccgagt tcgtggccgg
    21001 cgaggagatg gaccgatacg acggcttctg gtgggccccg gattcgcagc acgtgctgtt
    21061 cgagtccttc gacaccgccg acgagccgac ctggcacatc agcgatccgg ctgaccccga
    21121 gaagccggac gccggccgcc gttacccgcg cgcgctgacc cgcaatgccg acgtgtatct
    21181 gaccgtgatc acgctggcgt tcgatgagaa cgaccggtac gcaggcatca ccggcaacgc
    21241 cgatgtggat tgggatcgcg aggcgtacga gtatgtggcc gccgtgaact ggcgtcgcgg
    21301 ccacgatccg ctggtgctgg tgcagaaccg ccgtcagacc cgcgatcagg tgcttgaggt
    21361 ggcagtcgcg gcggatgggg ccgcgctggg tgccacgcgt gtgcttgagg agcacgcgaa
    21421 cgagcagtgg atcgacctgg ttcacggcac gcccgcctac acgccggatg gtcgtctggt
    21481 gtgctcgctc aacgatatgg ccacggacac caaccgtctg acaatggacg gccgcccgtt
    21541 cacgccggct ggctggaacg tgcgcacggt gctcgccgtg accgacgagg atgtgctggc
    21601 cgtggtgcag cgcgcgcccg aaatcgcgcc tgaggtgccg gacgcttggg ccgatgcggc
    21661 cgccaccgag tccgtattcg gcgaccacga cgcccgcagt ttcgacgtgg tgagtatcga
    21721 ttacaacggc acgatcacgc cggtcacgac cgatcccggc cagtggaccg cctcgcgcgg
    21781 cgagcgcggc atcgtggtct ccggccgcga tatgcgttcc gcccgcgccc agatgcggca
    21841 tattctggga gaacagagcg ccactatcgc gtccacggcg gccgaacccg gctttacacc
    21901 gaacgtcacg tttacgcgac tgggcgagca ccagctgtac acggcgatca tcgcgccgag
    21961 cccggaaagc ccgtacgcgc acgccgagaa gctgccggtg ctgatgaagc cgtacggcgg
    22021 gcccggcttc cagcaggtca tcgcctcgca gtccttctac tgggagggcc agtggtgggc
    22081 cgaccaaggc ttcctggtgg tcacggcaga cggccgtggc accaccggcc gcggcccggc
    22141 atgggaccgc gagatcttcg aggatatgaa ggacgtaacg ctggccgatc aggtcgaggc
    22201 cgtcaacgcc ctgcccgagg ccgtcgcccg gctcaacgcc gatgtggaat cacgtgcggc
    22261 acagccggct gcgggcgatc aggcagacgc cgagaaccac ccaccggcgc ttcgcgccac
    22321 ctcccgccag cgggaggcga ttcccatgcc ggacctcgac aaggtgtgca tgatcggctg
    22381 gtcgtacggc ggcttcctgt ccgcactggc cgtgctggac gcgccgaacg tgttcaaggc
    22441 cgcatgcgcc ggcgccccgc ccaccgactg gacgctgtac gacacgcact acaccgagcg
    22501 ctatctgggc ctcgacccgg atgtctacta ccgcaatggc atcgtgcagg acgcaccgaa
    22561 actcgagcgc ccgcttatgc tgatccacgg tttcgccgac gacaacgtca ccatcgccca
    22621 cagcctgcgc ctctcgcaag ccctgatggc ggcgggtcgc ccgcacacct tcctgccgtt
    22681 gacgggcatc acccatatga ccaatgacga gaccgtggcc gaaaacctgc tcaccctcca
    22741 gcgcgatttc ctgcgcgacg cgctggcctg acaaatcgat aacacgcgca tcccacccga
    22801 ggtgctccac cggtaaagca cctcgggtgg tgcgccgcac ccatttccgc cctgaatttt
    22861 tggatatttt gcactcatcg tcacgtgaaa gaccggtttt ttgtctcata ttcccatcac
    22921 cgcgtaggat gggtgaagat tagaaaggga ggactatgag cgttctcgac cgttttgaaa
    22981 aaagcgtgga gggtgcagtc aacggagtat tcgccaagtt tggctccaaa gacctgcagc
    23041 ctgtcgatct ttccagcgcc ttggagcgcg agattgacgc cgaggccatg cccgtgggcc
    23101 gtgaccgcac ggtggccccg aatgaatacc gtttcaagct cagtactcct gacttcgacc
    23161 gtatcgaggc atggggtagc gagaccctgg ccaacgaact ggccgataac ctcaccgagt
    23221 acgcgaagag ccagcattac gccttcgtgg gcccggtgat cgtgatcttc gaggaagacc
    23281 ttgacctgtc caagggcaac ttcaacctca cctccgaatc cgtgcagggc aatgccgtgc
    23341 cggtcaccac cgatgcgcag accgaggata gcccggtgct cgaggtgaac ggcaaccagt
    23401 acctgctcac caaagacaag acggtaatcg gccgcggttc cggttgcgat atcgtcatcg
    23461 acgatccggg catctcccgc aagcacctgg aaatcgacat cacggataac ggcgtgatcg
    23521 cccgcgacct gggttcgacc aacggcacgt acgtggaggg ccaccaagtg cccgccgcca
    23581 cgctgctcga cggcaacacc atcaccatcg gccgtacccg catcctgtac tgggcctccg
    23641 cccaagccca ggagtagtcg agcacacgcc cgaaagtcgt ccacttatga ccgaactgac
    23701 attcgccctg ctcaagtatg gattcctgat cctgctgtgg atattcatct ggctggccgt
    23761 acgttcgctg cgcaaggaca tcgagacgtt cagtccgcga ccttcgcgcg cgcgtcgtcg
    23821 ccgtgagcgt gaagtgcata aggccaaggc cgaaacgccg caggcagctg cgccgcgccc
    23881 gcatcgccag cccagcgctt ccgcgtcgtc cgccgcctcg gcgcagccta ccctgttggt
    23941 catcatcgat ggtccgcttg ccggcagctc cgtgccgctg gccgaagccg acatcacgct
    24001 tggccgcgcc gcctcgaaca ccgtggtgct cgatgacgaa ttcgtgtctt cccaccatgc
    24061 ccgtgtctac cgtgacacgc gctccgggca gtgggccatt gaagacctgg actccaccaa
    24121 tggcaccgtg gtcaaccagc agcgcatcaa ccgccccacc attctgcccg ctcgtattcc
    24181 tgtgcgcatc ggcgccacca ccttcgaact gaggtagccg catgccaaaa tccgccgctt
    24241 cacaaccgct gttcctgtat tccaccacgg tgtccgatgt gggcaccgtg cgcgcgaaca
    24301 atcaggattc ctccttcgcc ggcgagcatc tcatcgccat gtgcgacggc atgggcggtc
    24361 acgccggcgg cgatacggca tcctccatcg ccatccggtc gctggcccac atcgagcagg
    24421 atgacaccgg tggcaacgtc gagacgatct cccgcatgat ggaaacctcc gttatggccg
    24481 cgcatgacgc catcgtcggc aaggccaagc gcgaacgcaa gcttgccggc atgggcacca
    24541 ccgttaccgc agtggcattg gtggccggct actgggtcat cgcccatatc ggcgattcgc
    24601 gcgcctacct gctgcgcgac ggacgcctga gccgcatcac gtgcgaccac agctacgtgc
    24661 agcacctcat caacaccggt cgtatcaccc cgtccgaagc caagaaccac ccgcagcgca
    24721 acgtcgtgat gcgcgtgctt ggcgactttg acattgaccc gcgcccggat atttcggttc
    24781 gccgagccca ccccgccgac cgttggctac tgtgctcgga cggcctgtgc ggtgtgctgg
    24841 aagattccac catcgaagag acgatgacca cgctgtccga tcagggcgaa tgcgcgcagc
    24901 gtctggtgca gatggcactg cgggcgggca gcaccgataa tgtcacggcc gttattgcgg
    24961 atgccacgtt ggcgttggat gccgatgcgt tcgatctgcc gcaccaaacg ccgttggtcg
    25021 gcggtgccgc cagccgtaat cttgagccga ttgccgacat cgtcaacgag ccggtggcta
    25081 ccgcgcccgc actgcgtgag gatgattccc ccgcccagcg cgccgcagca ttgattcagc
    25141 ccgccagcgc gcacgaggcg gagaccacgc aaaccacggc caaaccgtcg tccacacgcg
    25201 tggtgcagcc gtccaccgtc cgcgaggaga ccggtgaacg agccaatccc gataccggtg
    25261 agattccggt ggtgcaaaag ccggatgggc gcctgtccgc cgaccccaac gacccggatg
    25321 tggccaaagc catccgcgat gagcaggtcg agcagaagaa ggccacgcgc accaagaagc
    25381 gccgcaataa gcttgcggtg attattacct gcattcttgc cgtgctggca ttggccggcg
    25441 ccggctgggg cgcgtacacg tggagccaga cgcactacta cgtgggagat agcaacggca
    25501 ccgtagccat ttatcgtggc gtgccgacca atctctttgg acttgagctt tcgcacgaag
    25561 tgcagaccac caatatcgcc accgcggacc tgcctcagac atggcgtgac cagctgagcc
    25621 aaggcatcac agtcggttcg ttggacgaag cgcagtcgca tgtgggtatt atccgcaccg
    25681 aaatgcgcaa actggccaag cagcaggaag ccgacgccgc caagaaggcc gaggcgtcca
    25741 attccccgga cagttccaaa gacagttccc agagcgattc caaggacggc accgaggatt
    25801 cgtccagcca gtccggctcc tcgtcagaca agtccggttc gtcaagctca tccgattcct
    25861 cggataaatc cggtatcgga gcggccgaca acgcggccac gggagattct caatgatcgc
    25921 tacccgtcta cgtcagattt ctttgttgct gattgccatg gcaatcagtg cgttggcgtt
    25981 catccagatg ttccagcgca ccgccggcga attccctatg caatacgccg gcatgctggc
    26041 attggtcgta gtgctgtttc tggtgctgtg ggggctgatt gtgcgcttcc agccgtacgc
    26101 cagccagtcg attctgcctt gcgtgctact gctgaccgcc actggcgtga ccatgatcgc
    26161 ccgcatcgac cagagcgaag gttgggcggt tgcccaacgt cagctgatct ggctgtgcat
    26221 cgccatcgtg ctttccgcgc tgctcatcat cttcctgaag gactaccgcg tgctgcggcg
    26281 cttctcctac gtgtcgatgg tggtgggctt ggtgctgctg ctcgccccga tgctgccgtt
    26341 tgtcggtcag gaagtgaacg gcgcgcgcat ttggatcagg attccgggac tgggccagtt
    26401 ccagccgggc gagttcgcca agctgttcct tgcgttcttc ttcgccgcct acctgttcga
    26461 ccatcgcgac cagctggccg tgggcggtaa gaagatgctc ggtctgcagc tgccgcgtat
    26521 caaggatctc ggcccgatca tcgtggtgtg gatcgcctcg atgggcgtgc tcatcatgca
    26581 gcatgatctg ggcacctcgc ttatgttctt cgccatgttc gtggccatgc tgtacacggc
    26641 caccggccgt aaaagctgga tcatcatcgg tctgatcgcg ttcgccgcgg gtgccgtgct
    26701 ggccgcaggc atgttcagcc acgtgggtca gcgagtcgat gcctggctgc acccgttcag
    26761 caacgagcaa tacaacaaga cccccggcgg ctcctggcag ctggtcaccg gcattttcgg
    26821 cctggcatca ggcggcatgc tcggcaccgg tctcggccaa ggccatcctt cgctcgttac
    26881 gtttgcgaac tccgatttca tctacgcctc tctgggcgag gaactcggtc tgatgggcgt
    26941 cctggccatt ttgatgctct acctgctcat cattgcctcc ggctttatca ccgccatgaa
    27001 aatcaaggat ggcttcggca agctgctcgc ttccggactg gtgttcacca tggccttcca
    27061 ggtgttcacc gtggtcggcg gcatcacgct ggtgattcct ctgaccggtc tgaccctgcc
    27121 ctatatggcg gccggcggct ccagtctgat cgccaactat attctcgcga ccctgctcat
    27181 catcatctcg aattcggcca atgcgcccga gccggagctg acctcggata cgttccagta
    27241 cgaggctttg gccgtgctgc gcaacaagga acttgaggcc cgggcccgcg ccactgagcc
    27301 gatcgttcag cctcgcagcg cgtcggctac ggcgtcgcag tccgagtcgg
//
LOCUS       DAWBMW010000007        23708 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_583_length_23708_cov_3.956623, whole genome shotgun sequence.
ACCESSION   DAWBMW010000007 DAWBMW010000000
VERSION     DAWBMW010000007.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 23708)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 23708)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..23708
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_583_length_23708_cov_3.956623"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1060..1159
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5233 a         7170 c         6339 g         4866 t
ORIGIN      
        1 caaccagctc cgcgccttca agggggcggg aacatcccct tctctcccgc cccctttttc
       61 ttttcttttt tgagctcaga acaatctgac gagcaggaat ggtgacggtg gccggtgaaa
      121 ccgcagtacg ggccggaaca accgttacga tgggtggcgt aatcgttcat ccagcaggaa
      181 gagtatggca atggcacggc gttggacccc gcagcggttt gttacactgc gtcgcatccg
      241 cgtaatcgcc tgtatcgcgg cgaccagtat cgcgctggct ggatcattcg cgttcaccgc
      301 gcgtaaatcc gtggccctga acatcaacgg gcaaaccacc caagtcacca catatgcgat
      361 gacggctacc cgtcttctgg aggaacaagg ggtggatatc aagacccacg atatcgtcac
      421 tacctcgtcc ggcgaccaac tcgccaatca tgccgtggtc accgtacagt ccgcctacca
      481 gaccaccatc aacatcgacg gcaccgccgt gccgttctgg accgtggcca ccagcgccga
      541 ccagctactg ggatttttcg aggaaaacca gaacaacgcc gcgaagatca ccgtcgacat
      601 caagaacgtg tacaaccagc tcaccggcgg cctggtgatc aaccagtccg ggccggtcac
      661 cgtcatcgcc gacggcaaaa gctcggtggc ccccaatggc aagctgccgg ccgccgccat
      721 cctcgattcc aagggcatcg tgctcaacaa ggaggatcgc gtcagcgttg agaaagacag
      781 cgacgagacc attctgcgcg tacgccgcgt gacccacggc gaggagacgc gcacgtccgt
      841 gattccgttc tccacccaga ccatcatcga cccgaatctg gctgaagggc aggccgatgt
      901 gcgccagcag ggcgtcaacg gcgagaagac gcaggtgtac gacgtcacct atgtggatgg
      961 cgtggccgaa tcggagacgc tgaaatcgga gaccgtcacc tcgatggcca tcgaccagat
     1021 catcgccgta ggcgccgcca agtctgatac cgataaaggn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnna ccggtaacac cggcaactcc ggcaacactg gtaacaccgg
     1201 cggcggtacg aacaacaatt ccggctcttc cggctccgat tcaggaacaa ccaccacgcc
     1261 ctccggccga ctctggcacg ccaccgtagc ccaggctcag gcctatgccg ccggtgccgc
     1321 cgcgcagcgc gggtggacag gcaacgactg ggattgcctg gtcaaactat ggaaccgcga
     1381 gtccagatgg ctgtggtatg ccgaaaacgc gagctccggt gcctacggca ttcctcagtc
     1441 gcgaccagca gacaaaatgg ctgcgttcgg tgccaattac cgtgacgatg ccgccgtaca
     1501 gatcgattgg ggtctgtggt acatcgcaca agcctacggc agcccgtcca aggcatggca
     1561 acattccgaa caagtcggtt ggtactgatc ggttacgaag cccacaagga acacacgctt
     1621 atgaacgaca ccattcccgc cactggtcac ctgttaggtg ccgccgatat ccgtcgtatc
     1681 gcggccgatg ccggcatcag ccccaccaag aaattcgggc agaacttcgt catcgacccc
     1741 ggcacggtcc gtcgtatcgt acgcgaggcg ggagtgaccg ctgccgatca tgtcatggaa
     1801 gtcggccccg gcctgggatc gttgacgctg gcgattttgg aaaccggcgc caccatgacc
     1861 gcagtcgaaa tcgatccgcc gctggccgaa cgactgccgg gcacagtcgc cgaattcatg
     1921 cccgaagcca cgtcacgact caccgtcgtc aatcgggatg ccttaaccgt caccccggag
     1981 aacgtgcctg atttcagcga tgacgcatcg tttacgctgg tcgccaatct gccgtacaac
     2041 gtggccacgc cgattctgct cacgctgttg gagcgtttcg acaacctcgg ctcgttcttg
     2101 gtgatggtgc aaaaagaggt ggccgacaga ctcgccgcca agcccggttc caagatctac
     2161 ggcacgccga gcgtgaagct cgcctggtat ggcaccgccg aacgggtggg caccatcggc
     2221 cgcaatgtgt tctggccggc tcccaatgtg gattccgcgc tcgtcaaatt cacccggtat
     2281 caggccgatg acccggctgc cccgggaact gcgaacagca cagacgacgg cacacaacgg
     2341 gaactcgtat ttcgtctgat cgatgcggca ttcggtcagc gtcgcaagac cctgcacgcg
     2401 gcattgaaga ccattgcgcc ctccgaggca ttctccatcg ccggcatcga tccgacccgt
     2461 cgaggagaga cgctcaccat cgccgaattc accgctctgg ccaaggccat cgaatcgtgt
     2521 ggagatggag atgaggcaca atgagtccgg taatctcaca tccacgctca gccgctgccc
     2581 gacatcagtc cgatgagctc tcccccatca ccattacggt ggattgcccg gcgaaaacca
     2641 atctcacctt ggaagtggga cctgctcacg acgaatgggg cggacgccac gaactggaca
     2701 ccatctactg cgcaatcggc gtatacgaca ccgtcaccgc aaccgccaag caacccggag
     2761 caggattctc tctggagctc gaaggcgctt atttgggcga cttggcctcc tcacgcagcg
     2821 acatgcgccg caaccatgcg gtactggccc tcttcgccat ggcccaggcg gccgagcgcg
     2881 agccggatgt ggcgttgacc atcaccaagc gcattcccgt gggtgccgga ctaggcggcg
     2941 gttcagccga cgccgcggcg accatgctgg cggtcaatcg tctctgggag ctgaactggc
     3001 ccatagagcg cctgcgcacc attgctgcca cgttgggcgc ggacatgccg ttctgcctga
     3061 ccggtggcct tgcatacggc accggtttcg gcgaacgcat cacggatatc gcccccggca
     3121 gccgcgatga gctggcgctg atagaacagg gattcagcgg cgaagtattg gtcggcgcat
     3181 accagtcgca actcagcact cctgaggtct accacacctt tgacatcgtg ggagccgcag
     3241 aaggcgaccg caatcatctg caggctgcgg caatcagcct gcatccccgc agcgggcagg
     3301 ccatcgacgc cgccacgcag gcgggtgcca gccatgcatt cgtctccgga tccggcccct
     3361 cagtggtcgc tttcgccgcc gacgaggcag cggcacaacg cattatcgag gtgtggcgcg
     3421 atacagctgt ggtcgatcgt attattcgag ccaaatctcc cgaacacccc aatatcagcg
     3481 tcagacagtg acgctaaggt agcaaccagc cacccgaccc atgaaagaag tccaccgatg
     3541 acgtatcagg ttgatgaacg tgaagcccgc aaggcgtacg tgcgccgccg ccagaccatc
     3601 gtcttttcca tcagcggcgc ggtattggca gtagtgctgg tcatagccct actgttcaat
     3661 ttccacgttt tcggcctcgg cgaagtggag actccggcca gccagccgaa ttacggcaac
     3721 gcggcgccgt gcgcggtcaa ggacagcagc ggcaaagcgc aatacgtgtc caacgcttcg
     3781 gtcggcattc gcgtgatgaa cggcacctct cacggccaat tcgccaaggc cgtgggtgaa
     3841 gctttggcca atcgtggatt cagcgtgcag aaaatcgata actattccag caccgacgtt
     3901 gaacgcacca ccatctactt cggcaagaac gccattaatc aggcgtatac gctgatcggc
     3961 aatttcaccg acgccaccat gatcatgacg gctcgcgagg atcagctcat cgatgtggtc
     4021 atcggcgcca cgttcaacga tctgcaggac accaactcct ccccgcaatc cggtaagacc
     4081 atcaccaata tcgagggttg caaggccgcc gatagcatga ccaagctgcc cgccgacacc
     4141 aaacacgacg catacacggc gcagtaaggc gcatacagct tcatcttcgc gaaaaaccgt
     4201 acgctgacac gtattcagcg tacggttttt tggtatcgga ctagtatcgg actactgaat
     4261 ctcggcgtac cagcgcttga gttcttcgac ggccacatcg tggtccaccg gaccgttatc
     4321 caagcggtat tccagcatgt gcttgtaggc gcggccgatc atcgggccgg gctccaggcc
     4381 cagcaactcc ataatttcgt tgccgtccag atccgggcgg atcgcgtcga agtcctcttt
     4441 cttcttgagt tcgcggacgc gctcctccat ctcatccatg gcctgggaga agatcatcgc
     4501 cttgcgtttg ttttgtgtgg tggcatccgc acgcgtgagc ctgttcagcc gctcatagag
     4561 gtgcccggca tccttgacat accggcgtac ggccgaatcg gtccacggct cgtccacata
     4621 gccgtggaag cgcaggtgca tattcaccag ttccgagacg tcctcgacca gatggtggtc
     4681 aaaacgcagc gccttcaaac gcttgcgggt cattttggca cccaccgcgt catggtggtg
     4741 gaagctcacc ttgccgcccg gctcgaaccg gcgggtcttc ggcttgccga tatcgtgcat
     4801 cacggcggcc aagcgcagcg tcaggtcggg ggcgggcacc gggccgtccg ggccggtttc
     4861 cagggcgata gcacgttcga gcacaatcat cgtgtgctcg aagacgtcct tgtggcggtg
     4921 gtgttcgtcg atctgcaatt gcagggccgg aatttccggg aagacgatgt ccgcaagacc
     4981 ggattcgacc aacgcctcaa ggccggcgcg cggacggtcg gagagcagca gtttggtcag
     5041 ttcgtcgcgc acacgctcgg cggatacgat gtcgatgcgg tcgcgcatcg aggtaatcgc
     5101 ctcggccgcg tccggcgcga tacggaagcc cagctgggcc acgaatcgca cggcgcgcat
     5161 catacgcaac ggatcgtcgt caaaggactg tcgcggatcc accggcgtgc gcaacacgcc
     5221 cttggacaga tcactggcac caccgaacgg gtccacgaat tgcaggtcgg gcacgcgcag
     5281 agccatcgcg ttgacggtga agtcgcggcg ggacaggtcg ccctcaagcg tgtcgccata
     5341 attgacctcg ggcttacgcg agtccggatc gtacgtgtca gagcgatagg tggtgacctc
     5401 caccttgacc tcggtgccgt ccgggcggcg gcgcatggca cccagcgtgc cgaacttgcg
     5461 ccccatgtcc cagaagccgt catgccccca ccggcgcagt atcggctcga actgctcagg
     5521 ccgtgcggat gagcagaagt ccaaatcgtg gctctttcta tggagcagca aatccctgac
     5581 cgggccgccg accaatgcca gctcgtaacc ctgttcggcg aacagtcgac caagttcgat
     5641 ggcttcaggc catacttcga aatccaatgg gcaccttccc gcgtgcgacg tttttctgcc
     5701 ctaccagcat accgttaccc cacctgcaca cggggttgag taaggtggag aatatgatta
     5761 cgcccgccga ccttgcccgc atgctggggc aaacaccaga ttcggcggat agccataccc
     5821 aagaccacct gctgtatacg tccgaaactc cgcatcattc cgccgattcg ggcgattctt
     5881 ccgatgtcgg cggcaccgcg agccactcca ccaatcaggc cgttcatcaa gacgaagtag
     5941 acgcagtacc gactgcgcaa cattccggtg tgcctggcac acccgcaacg cccgccacac
     6001 tcgaaccatc cgaatccgaa atatcggacg ccgccagcga catgcccgca ccgaagccga
     6061 tgatgcccac accggccacc gtcttcggca tgcatgccac cgtcaccatc gccactacca
     6121 gcggcactat cgaggggcag gctgatggta tcgccggcga ggccggtgcc gagacgattg
     6181 ccgaggtcga gccgattatc gccacggcac aaacgtccgt aaccatcgtc acgaacgatc
     6241 ccgcggcgac cgctcacgcg gccgtagatg ctgccgttgc ggtcgccaag gccgccgccg
     6301 cgcacaccac agcgataccg actccgcgcg acgtgctgat gccgcctgcg gcacgtaccg
     6361 caccggccgc aacgccggtc accatgccgg tctcagcgcc gtctgcagca ccagtgaggc
     6421 ccgcaacgtc agcgcatccc acgccggcca cggtcttcgg ccggcacccg tccggagatg
     6481 ttgcctcgcc ggaaccggca gtgccggtag aaccggtgac gccatccggc ccgactccgg
     6541 ccatgatgcc gcaacgccgt acgtcggacg gtccgaccac attcgcttca ctggatgccc
     6601 aggaactgcc ggtcgtacgc gaatattcgg caggcggtct gattttcgat gaccagaacc
     6661 gcgtggccat tatcgcccgt cattcccgct ccggccatct cgaatggtgc ttgccgaagg
     6721 ggcatatcga aaaaggcgag accccgcagc agaccgctgt gcgcgaagtg catgaagaga
     6781 ccggcatcct gggcgaggtg atcgattcca tcgccaccat cgactactgg ttcaccggta
     6841 ccacgcagcg cgtgcacaag ctggtccatc acttcgcgct caaacagacc ggcggtgaac
     6901 tgacggtgga aggagacccg gatcatgagg cggaagacgc catctgggtg cgttttgaag
     6961 atttggatga cgtgttgagc tatcccaacg aacgcaaaat cgcgtggctc tacgccagga
     7021 agaagaacag gcaggcaaac gagtgaacct acccggagtc ttacgacatt gcacacgtct
     7081 ggcacacgca ctggcggctg gcgtcgccgt tgtggcgatg ctggccgctc cattgaccgt
     7141 ctacgcgaac gatacgacgg acatgactga ttcatccgat acgacggacg caacgggcaa
     7201 tcagaccgag aatcagccca agcagagcat tgccatcacg caaagcaccc ccatcgtcac
     7261 ctccaaatcg ggctatcgtg tcaccgcggt catcagcaat acggacagca cggccacgtc
     7321 aagcggcaca ctgcacctga ccaccaatgc gttgttcacc tttgtctcgc gcaccgatat
     7381 tcagaattgg gccgagaaca acgcccccat cccaaccccc aacgagttgg gttccgttga
     7441 cgtgccatcg attcccgccg gcggcactgc caccgtcacc atcgaggtga acgccaatca
     7501 gcagaccctt gcctccatag ggagttgggg accgaaaccg ctgatgctgt cgtatatcgt
     7561 tggcaatcag atactcgccg atttgccgag ttttctgacc cgatcttcgg atgggctcaa
     7621 caccgcgaca acaccggcca tgaacctgac cgtggccatg ccctacaccg ccagcggctg
     7681 gcaggtcgat aataaggcca tcagcaatcg cattgccgaa accaccactt cctcgacatc
     7741 aacatcgtcc aagtccaaca gcacggacaa ggccgacaag tccgataacg ccgacgccag
     7801 caacgcggcc aaagccactg atacgcaagc catatcctta tccgaacagg atgccgatac
     7861 cgcgcgatcc cttgggcaga cattcgccaa gcaccgctcg ctgcaagtga tcgcggatcc
     7921 tctgtattgg cgtcagtccg cagtgcaacc gtccattgcc ggcatcatgc agcccgccga
     7981 cttcgacatc accgcctata ccgcactgga cgataccgcg gcctacgaaa aggccggcat
     8041 caccactgag cagtggaacg ccaagcaggc ggccgcatgg tatgccgacg gaggcgactc
     8101 cgaaacaccc tccgcgtacg catggcaggg caacaactgt tggacgttgg atgcgctgac
     8161 cgcagcacgc gagcagggct atgacacggt gattgccgat gcctctttcg atgccgacca
     8221 gactgaagcc gtacacacag gcacctacgt ggtgcatacg cctgccggcg atgtcaccgt
     8281 gctgaaggag caatcgacct tgggcacgct ggccaagggc caagccacca gcacggacgc
     8341 gcaggccgag tccagcgatg ccggtcggtt ggcccgactg atcgctcaga gcgcgttcta
     8401 ccagatggaa cagccctata catcccgata cctgcttatg acgttctccc gcaccacgga
     8461 ggccagctgg atcgatcagg tgatgagcgc cttcgagcag gcgtcttggt tgaatctgac
     8521 ggacctgaag accatggcca aggccgatcc gtacaatgtc agcgattcgg tcaatcccga
     8581 caaggccgat gatgccaaca ccgccaatac ccggtcggca ttgagacagt tggccgacag
     8641 ccggcatgac atcatgcgca tggccacctc catattgagg aacgagattg attccgacga
     8701 ggtctcgtcg ttggacccgc aggccctggc ccggcaggac gccaatgaca ccgcctcgca
     8761 tagcaacgat ccgacgcaat ggattggctc tttactggcg ctgcatgacg atatggcgct
     8821 gcggtccatg tctggctccc cccagccgac agccacgcga aaagccatgg tcaaagccac
     8881 gaaaacactg gccagcgatc ttctgaacgg cgtacgcatc aacccatcgg aatcaatatc
     8941 cgtgttcagc gaatccgcga aaatgccaat caccgtcagc aatgaccttc cctacgccgt
     9001 cagcgtgcag gtcaactcac tgactgactc catgcagatc gtcacctcgc gaaccgctga
     9061 catcgatatt ccctcgcata gcgacgctca ggtgaccttc accatccgcg tttccacttc
     9121 cggctcatcg acggcccatg tgtcgttgac cgaccgggaa ggcaacagct tcggcaacac
     9181 gcaggacacc gcgatcacca gtgtgatgcg catcagtgat gccagcggct tcattatcat
     9241 cggcttcgcc gtgctactgg gcattatcgg cctgtggcgg cagttccatc ggaagaagga
     9301 ccccgacgaa tgagttcaag cgtaggtcgt aattccctga tcatggccac gggaaccgct
     9361 gcttcccgtg tgaccggtca gctccgcacg attcttctcg ctgcggccat cggcaccacc
     9421 ggattggcgg ccaacgccta tcaggccggt tccatgattc cccaatccgt gttcacgctg
     9481 gtgtccggcg gcatcttcaa cgccgtgctg gtgccccaga tcgtgcgcac gttgaaagaa
     9541 aaagacgccc aagaacgact gaatcgcctg attacgttgg ccatcggcat cctattggcc
     9601 atgaccgtgg tgatggcggc ggccagccct ctgcttgccc gcctgtacgt gggtagcgac
     9661 gatcaccaga tgatagccct gaccacctcg ttcaccctgt ggtgcatgcc tcaggtgttc
     9721 ttctacggcc tgtatacggt attgggtcag atcctggccg ccaaagacca tttcctcacg
     9781 tatgcgtgga gctcaaccgg tgccaacatt attagttgcg ccggttttac tggctttatt
     9841 ctgctgttca gcaaggccaa tgaacagccc ctcgaattct ggaccgccga caaaatcgcg
     9901 cttaccgcag gcacctggac tttgggcgtg gccttccaag cgctgatact gttcctgccg
     9961 ctggcccgta tcgggttcaa gtacaaaccc agcttcgggc ttggcggctt tggactcagg
    10021 tctatgggtc ccgtggcgtt aggctctttg ggcgtggtga tcaccagtga gatctgcggc
    10081 attatcctta cccgtatcgc cacatccgca ccgcagcgcg cccacgaact gacgggtgcc
    10141 agcctgttca gcgtggcagg caacgcgacc tatcagaatg cgtacacact gttcatcctg
    10201 ccctattcac tcatcgccgt atcggtatcc acggctatgt tccccaaaat ctcccgttcc
    10261 atcgcagctg cgaatctgga tgaggcacgg cacgacctgg tcagcgcact gaataatgtg
    10321 ggcctgctca tcatgttctt cgccgccgcc atggtcgtgt tccccgaacc gatcatccgt
    10381 gcattgcttc cgtccgtctc gatggacgag actatgttga tctcctatgc gcttatcgcc
    10441 ttgtctatgg gcattccatt ggggtcggcg ttcctgctga tccagcgcac gttctacgcc
    10501 tttgaggacg gccttcaccc cttcctgtcc gcggtgatgc agtatggatt cacctcaata
    10561 ttcatgatca tcggcatgat ggtacttcct cctgagcatt gggtactcgg catcgcctgc
    10621 tcggtgaccc tcggtggatt actggcgttg ccgctgacgt tgatgatgtt gcgccgcaag
    10681 ttcgaaggca atcttggcgg tcgggagatc acgaggacat atgccaaggc cattgtggcg
    10741 gcactggctt ccggagttgt ggtctggctg atcaaacgac cggtagtcgc actgttcggc
    10801 gcggatattc gaccagtagg cgggcacatg agctggtggt ctgcgctggc catctgcgtg
    10861 gtgctcacca ttgtgctggc cgtagtatat gtggctgtgt tgtgggtggt gcacaccccg
    10921 gaactcatca gcgcctatca ctcgatactg gcccgtctac acaagactcc ggccagtgag
    10981 gacgttgctg tgacagccca gccggaaaaa agtcgacgat acgcaaatca atcctgagac
    11041 ggagcccgtc aaggatagcc ccgcacgtag aatatctaag accagtctcg aacaccaatc
    11101 acacggagcc gaacgcacta tgaaaccgca actcggagac acgattctca accgatacac
    11161 cttggtgtca tcgttgcgtg aggagcctgg cattcaggca tggaaagcca acgaccgtgt
    11221 gctggcaaag gattgccagc tgttcatcat taccaatcgc agcgctttcg ccgcaatcga
    11281 tgagctcgct ggtcgcatgg ccgccactcg ccctgatcgt gtgattcccg tactgaaata
    11341 ccgtatggaa tccgacgtac ttctcattgt catgccggtg gatagcggcc aatgcctgac
    11401 tgactacatg gtggataccg ccaagccgct cagctacagc gccatccggt cgattatcgg
    11461 agaatgcgcc aacgtgctgc gcgaggtcat tgcggatact cccagcggca tcgtcatcac
    11521 aaccgacacc gttcgagtga ctacctccgg tgtgcaaatt gccgatgcac catgtgccac
    11581 gatgctggcc gatacgtccg ctaccgatct gcgtacggac ggtcctgaac ggtatgcgat
    11641 tcgccagctt gcggcattac tctataccct gctgacacgc acgccaagcc aagcgacacc
    11701 cacattcaat ctgcgtgcct tgccgcaaga cacccctggc gaattccgcg tgatttgcaa
    11761 gcgtggattg gccttgagcg agcccgacga gcatacgttg ccaatggcag cccttgtaga
    11821 actcgacgcc ttgttgggta attggaagcc gctctctgaa ctgagcgatg ctgatattgc
    11881 tttgccgagc gtggaaagcg attgctcgat taccaaggcg atactgaagc ctgccaacga
    11941 gactgatatc gtgcctattc ccagcacgtt gattaacagc aacagtcttc cggaattgtc
    12001 tattacccct tccctgccgg ttcagccggc cgccgtcccc gctccgcacg cgatgtcacc
    12061 ggttcctctg cctgatgggt ccagtactgc tgacggcaac cagtctcagt ctgatgcccg
    12121 ccgtctgttt gacttcaacc tttctgacgc ctgggagcac gaaaatcttt ccggtgaagg
    12181 caccggtgac tggtacagcg aaatgaatcc ggcccctatc ggcggtccgg gtgataatac
    12241 tcggttgacg gtgcctattg ttaccggcgg tatttccgat gccaccgtgg gagagacgac
    12301 cagtcgtatt cccgtgttcg acgccagcgg ccgtgctgtg gctcccggtg aggaatcact
    12361 gcgtgccttg gaagaggagc aggcccgcat tgccgaagta agcgccattc ctccgagctt
    12421 cacaccgaag gatgtgccgg atgacgaggt tgacagcaag ctgcccgatg aacatctgtt
    12481 cggcaaactg tcgaccaagg tcgttgccat cattgtggct ctgatattgg tagtggcagc
    12541 ggctgtttgg gcagtgcatg cgttccgatc ctccggagac aatcctgccg atgcttccag
    12601 caactccagt gaaggtgact ggcctgatat caatgctgac gctgtgccat ttggtggcag
    12661 cgagaacgcg acgaacggtg gcaccactga cgattcttca aaatcgagtg atgccactca
    12721 gagcgaatcc agggactcag catcagacaa gtccaagaag tccgagactg ctgattcgtc
    12781 gtccaagaag accaaggcca agaagtccgg cgataaggat tccagcgctg ctccgcaacc
    12841 gcgccatgag aacaacacgg cgtttacgat ttccagcgcc aacttcctgt cgaatcctgg
    12901 cggccaatcc gggtacgcat actacatgca ccttgaccag ccgcaggatg cgtaccgcat
    12961 ggttatcacc atccgcacat ccggtggtaa gggctatatt cgcgcaaata ccactggaga
    13021 ccccactcag ggcgagcagg tggccgaatt cacctttgct gaaggtggca ctactgaggt
    13081 gaagttcacc aaagccgtca atacgcagga tctgctgctg tgggtgccat cgggtagcct
    13141 gccgcagaac cagctgtaca tcgagaaggt ggaggtcttc taatcggcat ctatgccgat
    13201 aactgaccca agaagggcgg taccgttcgc atacattcga cggtgccgcc ctttcttcgc
    13261 gtctggctga atgacatgga atatcgtatg ggggtgtgac aatgtttcac gtgaaacata
    13321 acgtcatcat tatcggttct ggtcccgctg gctacacggc cgccatctat cttggccgtg
    13381 caggtcttaa tcctgttatg gtcactggtg cgttgtcgcc aggtggacag ttggtcaaca
    13441 ccactgaggt ggagaacttc cccggattcc cggaaggtat tcttggcccg gatcttatgg
    13501 atcgcatgaa ggaacaggcc aagcgcttcg gtacaaccta tatcacagac gatgtctctt
    13561 ccattgaggc gtgtgagagt gattcggtca agccgaccta tcgtgtaacg ctctccgatg
    13621 actcccagtt ggaggcatca gcactcatca tcgccacagg gtcgtcattc cgtaagcttg
    13681 gggtgcccgg cgaacaggag ctgtccggac atggcgtctc gtactgcgcc acctgcgatg
    13741 gcttcttctt caggaacaag cctatcgtgg tcgtaggcgg tggcgattcg gcttttgaag
    13801 aggcattatt cctgacccgg ttcggttctt cggtcacctt gattcaccga cgcgatagct
    13861 tccgtgcctc gcagatcatg gtggaccgag ccaaagccaa tccgaccatc accctactga
    13921 ccaacacagt ggtgacctcc attaccggca cctcctcccc cacacagaac accggtgcac
    13981 ctattgccat tcctggcttg acaatcaaac gaccggctgt ggcaccggcc agtgtcagca
    14041 gcattgccgt acgtaacgtg gtgaccggag aagaaagcac gctggacacc aacgccgtat
    14101 tcgtggctat cggccatacg ccagccactg attttgcagc tggtgttgtg gaccgtgaca
    14161 atgatgggta tgtcgtggtg cagggtgcca gcacggtgac cagtgcgcca ggcatctttg
    14221 ctgcaggcga ttgcgtggat cgtacttatc gtcaggcaat cagcgcagcg ggcatgggct
    14281 gccgtgcagc gctggatgcg caggcctatc ttacggactg atggtggcgg actgatggtg
    14341 gcttgctgtt ctggctgctg cctgtgacat aggagcaacg ctaggtcagt gttacagcat
    14401 gccgcccttg gatagtacgt atcggtcaaa ggcatagaaa gacccctcag gcgacggaga
    14461 atcacgatgt gtgattgtcc ctccagcctg aggggttttc tattgcgaca ggggctacag
    14521 cgcgggcata cgcccctgcc aggaatcacg cagaggctat aacgtcttgc gcatacaacg
    14581 cactgcacag caataatgcc ggcgacaaca cgaatgttct tatcgtgtta cacccatttg
    14641 gaatcggcat cgccttggct cggcaccagc agctgcagga tgcgatccat atcctctggt
    14701 gacgaaaaga cgatttcgat gcgaccatgc ttggtgctgc ccttaatcga caccttggta
    14761 tcgaaatgat tttccaaact ctgctggatg gcggaaccaa cccaaggatt ggtcttagac
    14821 gtcttaggct tcttcggctg ctcccccgac gcaatcttca tggcgacgat ttcttcggtg
    14881 ctgcgtacag aaagaccttc cgcaataata cgagaagcca gcttgtccat ttcctccgga
    14941 tcgctcaggc ccaatagcgc acgcgcatga ccggacgata gcacaccggc agccactctc
    15001 ttttgcacag aagccggcag gttcagcagt cgcaaggtat tggcgatttg cgggcgagac
    15061 ttggacaccg acttggacag ttgtacttgt gtcaacccga actcttcaat catctgctgg
    15121 taggcagccg cctcttccag cggattcaag gctacacggt gcaagttttc cagcaatgca
    15181 tcgcggagca tatcatcatc cgcagtggtt ttgacgatgg cgggaatcgt ctccaaccca
    15241 gccaactgag aagcacgcca acggcgttca cccataatga gctcatacgg gctgtccaaa
    15301 tgaccggcaa acggattcgt gctggattcc gtctcgtcgt gcttggcggc tttggccgca
    15361 gcaatctggc ttgccggacg cttgcgcacg actatcggtt gcagcacacc aacttcctta
    15421 atggaagcag acagctcatt caactcatcc tcgtcgaaga tggtacgagg ctgatgtgca
    15481 ttgggcccga tatcagacag tttgagttca gccagataac caccggctac cggcttgagc
    15541 tcgtccttct tatctgtgtc agcggtcttt gccgccgatg cagtcaaaga tgtttcacgt
    15601 gaaacatctt tgacagtgac ggatgtacga tgtgccgtct gagacgcaga cgacttgccg
    15661 ccctgctgag gtgcggcgac cttaccggtt tgaggtttct ccgtaccgaa aaacaggtcg
    15721 ctaggatgag taatggtgtc caatgcgggc atggatgcgc gtttaccggc ggctttcttg
    15781 actgccttgc tggacgatat cgtctgaggc acggcaggtt ttgattcctg ctgggcagta
    15841 gccttcttgg cgggggttat gtcagcagtg gcctccggag tagacgaatc ctccgcaatc
    15901 attgcgggct gctccgccac cggctcctcc cctggcaatg tggggaacaa agcgccaagg
    15961 cccttaccaa gtcgtgattt tgatgccatt acttgttcct cctcgcatca atggtgtcaa
    16021 ggacttgttg cgaacgctgt gcgatttcca aagctgcttc accatacgat atggcaccta
    16081 tgccacgagg atcataggca atcaccgatt gggagaaact cggcgcttcg gagatcttga
    16141 ctgaacgcgg gatggtggta ttcagcacaa tgctgggata gtgtccctta acctcgttat
    16201 atacttcacg gctcaataac gttcggcgat cgaacatcgt gacgagcatg gtggacacca
    16261 gcagtacggg attgaaatgc tcttgcacca aaccaatcgt gttaatcagc tggccaagac
    16321 cctccaatgc gtaatactca gcctggatag gaatcagcat ctcggtcact gcacacatgg
    16381 cgttgatgac caagaggccc aagctgggcg ggcaatcaat gaacacgtaa tcatagtgtt
    16441 tatctgattg acgtaggtag aggtctaggg cctccttcag caacgtgttt cggttgggca
    16501 gatcagccac ttccaactca gcaccgctga gatcgattga tgcaggcaca acatcaagtg
    16561 tgggaaaatc aggacagacc gtgataacat cacccagatc ggaacgcccc tcgataacgt
    16621 catagaccga gggctctccc gatgcatgag atacacccaa cgccgtagag gcattgccct
    16681 gcgggtccat atcgatgacc aacacctgcg cgcccttgtt cgccagcgcc gcagcggtgt
    16741 ttaccgtgga tgtggtttta cccactcccc ccttctggtt ggccacggcg atacgacgag
    16801 tccgttgagg ctttgggaaa tcggttttct tgagtacatc gtagcgtgag ctttcatcag
    16861 ccatttggct accaagcact gaaccgtgac catcgaatat tcgctgaagc gtttcggaag
    16921 ccgattctat aggttccgac tgatgatcct tggtggccat cgctcctcct tgcacagacg
    16981 taagttccag tgttctcatt cgtatggaca tgatgctcgt tatccacaat tgcgatatgc
    17041 aatgtggata agtgtggata agtgtgaatg acgttgagtg ttagattgtg tattcacaga
    17101 cgaaaaacat tgaaaaatca atgttttcat cattatgcac atcgtcccgt aaaaatgtgg
    17161 ataactttta cgcacatatg gaaatgtgga taactcaatc agtactccac tcatcgctta
    17221 tccacaatca ccacgtgggt aggctcaaga tccggcccga cagccgcctc aacgacacgt
    17281 ggacgacgac caccgaattt tgcaatctcc ttgccagcct tgtcgatttc agcctgagca
    17341 gaccgtccct tcagtgcgat caactgtcct gacggtttca gcaacggcaa cgtccagcca
    17401 gagagtttgg tcataggagc aaccgcacga cacgtgacaa cagcgaacgg atgaatttcc
    17461 cgcttccgta cctgctggat gacggcatcg gagcgtccac gaataatctc aacgttctgc
    17521 agttgcatca ggccaacaca ttcatgcaac cattcgattc gccgctccat cggttcaatc
    17581 agcgtgaagt catgatccgg caaacacgcg gcagccacca atccaggaaa accaccacca
    17641 ctgccgacgt cggcaactgt tttgaagcga ataccattcg tcgcattccg aacgtacggc
    17701 acaatcgcag cggaattcag aatatgccgt tcccaaatga tatcgacgtc acgggggccg
    17761 atcaatccac gaggttcacc ctcttcggca atcttggcat ggaaacgctc caacttcggc
    17821 aacgcatcac ccaagacctc agcaagtaca ggtgacccct gcagctgatc cgtcatagcc
    17881 atctccctca ccatgtttca cgtgaaacaa tcaacatgga gaatggcgcc gctcattacg
    17941 acgccattct ccatgcggtc actttagttt gttcaaagac gacatccgtc gaacgtccct
    18001 gtcatctcaa aaaaactcgc cgagcaatta ctcctgatca agctcgtcgt cttcggcggt
    18061 atcctcgact ctattcttga gatacaccgt cacgtaacga tgaggctcct caccgtgcga
    18121 gcgagacttc agcccctctt cccgaaccac atcgtgaacg actttacgtt cgaaggaatt
    18181 catcggcttc aagtcatacg gctcgccgga atccttcact gcgtcaatgg catccaaggc
    18241 gatatcacgg aggtgctgac gcttgcgctt caggaaaccg tcgacatcca caatcagatg
    18301 agaacgctcg ccggtcttct gctgtacagc cagacgagtc aattgctgca gagcatcaac
    18361 gacttcacca ttacggccaa taagatgttt gatgtcagta tcgtcatcag caacgatttg
    18421 cacggtgggg cggtcattac gcacgcccat ctcaatgtcg ccctcatagt cggcgatatc
    18481 cagtaggcct tcaaggtaat ccgcggcgat gtcagcctct tcgttgagct ggtcgatcga
    18541 cttcacgtca tcctgtgcca tacgctactc cttcatgtcg tgtttgaatt ccaagtctaa
    18601 gccctgctcc cccgaacaga gaaagtaatg tcggtgcgtg ttgaatgttt cacgtgaaac
    18661 atcgaactcg caccgacata gtcgtcactt ctttttgcgc ttacgcacgg gctgttgacg
    18721 ctgatagccc tgagtggcct tacgctcggc ctcttccttg gccttggcca atgcttcttc
    18781 ttccagagag ggcaggccgg cctttgcgcg gcgagcattc tcattacgat gatcgcgctt
    18841 ctccttgtcc gccgcagccg gagagccagg ggtcgggaat gcgtacacct gccacaatgt
    18901 gcggcagagg ttgcacacgt tgttggtcag ccagtagacc agcacggcga acggcatggc
    18961 gacaccggag aagatgtaca tcaccgggaa gagccacagc atagccttct gcatggtctc
    19021 ggtctgcttg ttcatcgaag cagctgccat gttgcgcttc atgctgaagt actgcataaa
    19081 ccacaggcag aaacacatca ggaacacgaa gataccgatg accgccttgc cactagcggc
    19141 agcactggtg aagttatcgg tcacgctgac aatgccgaac acatcggttt gggtgaattg
    19201 cttggcggtt gcgacgtcga acgcgcccaa cgcatcgcgt ttaccgttgg caatgtatgg
    19261 aatggccgac agcgtataga acatggacat gaacaccggg ccctgaatga gcataggcaa
    19321 gcaggagcca gccggattga cgtcattgtc ttgatacagt ttcatggttt cacggctcat
    19381 ggcctccctg gaagcctgat ccgtcttgcc tttgtacttg ttctgaatac gctgcatctt
    19441 cggcgccaat gcctgcatct tgcgcatcga cttcatctgc ttgtagaaca gcgggaagat
    19501 acaagcctgc accaccagca ccaggatgat aatggcaagc acccatgaga caccaatcgg
    19561 gctcacgccc agcatcacga agaagtcatg cacgatcttc atgatccagg tctgcaacca
    19621 ctcaacggga gtcaggattt tatatagaaa tccccaaaac ccgctatcaa gcagaaattc
    19681 atttgagttc atcgttgggc ctctctttgg gttgtggcca atggtgtcag tcgaggctct
    19741 tcatgagcct tcgaccacga aaacctgtaa aaaacggaat accgttgtgg aacatcgtcg
    19801 attcccccac gactccacgg ccggcaacgc agtaatctca gcacggcgag cactccccct
    19861 ttgaacgcgc cataccgttc caatgcctca atggcataat tcgagcacgt tggataatac
    19921 ttgcaacaag gcggtgtgtt tgccgagatg tgctgttgat accagcgcac cccaccaatc
    19981 atcaccgcct tgaaagaagc tgtcatgcct cagccacctt atgcgccacc gtggcaaaag
    20041 ccttcgcgat ctgctgatct agcgaagcga acgatgcggt cgctgcactg ggcttggcgc
    20101 gcaaaacgat atcgcaatgc gcgggcagca gatcctcatg ggctctggcc aatacacgaa
    20161 acctacgctt caccgtatta cgggtgaccg catgtcctac cgatttggag acagctaatc
    20221 ccaaacggcg atgcaccgtc cggtcgtcat catcatgatg gtcgtcgggc accaaatagt
    20281 gcacgacgat atccttgcca cctgctttgc gccggcgttt cagcacggtg acgaaatcac
    20341 gatggctttg aagcctctcc acctcgacta tgcctgtggc actgatcgga atgatcaggc
    20401 ggagagggac ttgcggccct tggcacggcg acggttaatc accgcacggc cagaacgggt
    20461 gcgcatgcgc aggcggaagc cgtgcttcat gtggcgacga cggttgttcg gctggaacgt
    20521 cctcttcata atccattgct cctaggtcta ttcggcaacg cttcgcgctg ccctcacgtg
    20581 agtcaagcta taccaaagta ctctcacccc taccctcaag tcaaaacgga cggagtattt
    20641 tcacatttcg acacgttgcg agacacgccc caaatctctt ataatcggcg gaataccaac
    20701 cattcacggc caaaagttat ccacaaatta catgtttgtt attcacaatc cagttatttc
    20761 aacacgcgac acggtggata acttgtctaa accagagtaa atattatctg ttgtacgtat
    20821 ttcgccacag cgacagaagg ggccaccatg gttaacgctt ccggcgaccc cgtaatcgag
    20881 gccgcccaca tctggtcaga cacgctgacg gtgctcaaac acagcgcttc gctcagccca
    20941 cgagaaaaag gctggttgga aggcgttgtt cctgaaggcg tcttcggttc gaccatcgtg
    21001 ctgtgtgtgg acaacaacga cacgcttcaa gccattcagg gtgatttgaa cgattccctg
    21061 cttcaggcat tgcgtacggt aaccggcgaa aatatgtttc ccgcgttcaa ggtcgtgccg
    21121 aaaaccgagc ccgaaccgct ttcggaggca aagccggcgc agccatatcc ttcggaaatt
    21181 tctccgactg tcgccgaatt cggtaaagaa tcctatggtg caaagccggc agccgcaccg
    21241 cgggaaccga tgcccgccac cgagccgcag ttcccggtcg gtcagcagaa gatgaatcgc
    21301 gatcccgaga ctcatctcaa taagaacttc acgtttgatt ccttcgtgcc cggcgattcc
    21361 aaccgttttg cccgtacggt cgcactggcc gtggccgaag gttccgggca ggatttcaac
    21421 ccgttgtgta tctatggcgg atcgggcctg ggcaaaaccc acttgctgaa cgccatcggc
    21481 aactatgcac tggtcaagga tcccgggctg aaggttcgat acgtcacgtc tgaggaattc
    21541 acgaacgaat tcattgacgc gctgcaaaac ccgaaccaaa gccagggtca gattgctgaa
    21601 ttcaaccgtc gataccgaca ggttgatgtg ttgcttattg acgatatcca gttcctcggc
    21661 ggcaaggaag ccaccttgga tcagttcttc catacgttca atgcactgca tcaggcaaat
    21721 aagcgtattg tcattgcttc cgatgtagcg cccaagaatt tgaagggttt cgaggctcga
    21781 ctcatctcgc gcttcgaatc cggcctgacc gttgacgtca agccgccgga tctggagacc
    21841 cgaatcgcca ttcttcgcat gatcgcctcg atgaacggct cgaaaatccc cagcgatgtg
    21901 ttggacctca tcgccgaacg cttcaccgag aacattcgtg aactcgaagg cgcgctaacc
    21961 cgtgttacgg ccgtggcctc gctgagtaat cagccggtga cccgcgcctt ggccgagcag
    22021 acgttgcagg acttcttcac caccgatgtg gaaatcaagc ccacggacat catcagtcag
    22081 gtggcgaagt acttccacct gacgtttgag gatcttgtcg gtaaatcacg aaccaaaaac
    22141 gtggctgttc ctcgtcagat cgccatgtat ctggctcgcg aaatgaccag tatgagcctg
    22201 atggatattg gccaggtctt tggcggacgc gatcacacca cagtaatgca tgcctgcact
    22261 cgaatcagtg accgaatgca gcaaaaacag gagatctata actacgttat ggagctcacg
    22321 gttcgcctca agcaaagcaa caccaactga cacaacgctt ctgagagacg aaaaacggca
    22381 ctttttggac tgactttccg aaaagtgccg tttttggttg ctcaggcgga ttctggtgga
    22441 tacgtcgagg caacggattc gagacattag attcctcatg gctccccttt gtgaggggat
    22501 ctgtcggcgc agacgactga ggggagtttc gaaggggaac acgcaaaaaa accatgaaac
    22561 gagagaatcg attctgagga ccgattttgc gggtttgggc gagcgagacg gaaaaagtgc
    22621 cgaaaacggt ctccagaatc gattctcctc gacgaaaaac agtcttcaac acgccgtgcg
    22681 aaagtgcgaa aaccaaaaaa agttatccac atagttgtgc acagatgtgg gtaaactcgg
    22741 cggataactc gtggattacc cactattttg ttgtggaaaa ctcgtgcata accgcgacct
    22801 cgatgtggaa aactcaaatt cgacgaactg gctgtggaaa actcgcccac ttatccacaa
    22861 gccatgcaga atttgtccac aattttcagg gacttatgaa ccgttgcagt tgtagggctc
    22921 agccctactt atccacacta tccaccggcc ttattatggc gattacttac tcagttacgt
    22981 gaaaagcatc atcgccatag taacagccgg tcatccagcg tacgtaagtt cgccacctaa
    23041 cggctagaat attcctcagc caatccgacg aagggaacaa tcacacatga aagtcgaaat
    23101 cgataccgca gccctggccg acgccgtagc ctggacctcc cgagtcatcg acgcacgtcc
    23161 ctccaacccg attctcgctg gcgtcaagct ggaagccatt gacggaacgc tgcaattctc
    23221 cgccttcaac tacgagattt ccgctcgcca ccacatcgaa gccggcgttg acgaagccgg
    23281 ttccgtactg gttcagggca agctgctggc cgatatcacc aaatcgctgc gttcggagaa
    23341 aacgtacctg gtcaccgacg gctccacgct gaccatcacg tccggcaagt cgaagttcac
    23401 cttgcagctc atgcccgaca ccgagtaccc ggatctgccc gtggtaccgg ccatgctcgg
    23461 ccaagtcgat gccccgactt tcgttcaggc cgtgaaccag gcgtgcgttg ccgtctcccg
    23521 cgaagaaaac cgcccggtac tgaccggcgt tcgcatgcaa ttccaaggcg acaaggtcgt
    23581 catgacctcc accgatcgct tccgtttggc ccgcgccacc ttcacttgga ctccgcagaa
    23641 cccggacgtc gacaccacca cgctggttcg cggctccctg ctcaaggatg tggcccgtgc
    23701 tcttgacg
//
LOCUS       DAWBMW010000008        23693 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_584_length_23693_cov_3.668669, whole genome shotgun sequence.
ACCESSION   DAWBMW010000008 DAWBMW010000000
VERSION     DAWBMW010000008.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 23693)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 23693)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..23693
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_584_length_23693_cov_3.668669"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         4917 a         7308 c         7023 g         4445 t
ORIGIN      
        1 atcgtggata ccaccactcc gtcgtatgcc gaagccgatg aaccgtacac gcctaccggt
       61 acgattcccc cgcttcctcc cgttaacgga ggcactcgcg tatgaacaaa tatcttcgtc
      121 agcttttcac cgccgtggtc gcgctgttcg tgattctcgg cctgtcgtcc accatcatca
      181 tggcggtacg ggccaacgcg ctgaacaacg atccgcgcaa cgcccgttcg ctgtaccacc
      241 agctcggcgc ctaccgcggt gccattctgg cctcggacgg cacggtcatg gccaaatccg
      301 agccggtcaa cgacgcgttc aaatacctgc gcacctattc caacggtccg ctgtatgctc
      361 cggtcaccgg cttcttctcc atcaaccaga ccgccgatcg cggcctggaa gcctcccgca
      421 gcatgctgct caacggtgag gccgattcat tgatctggca gcgggccaag gccatgctca
      481 ccggcgctac ggatcagggc gcgtccatcg aaacctccat cgaccccaag ctgcagcagg
      541 ttgcctacga tcagctcggt acgcgcgatg gtgcggcggt ggccatcgaa gtcagcaccg
      601 gtcgtatcct cgccatggtg agcacgccca gttatgatcc gaacaaactg gcgacccatg
      661 acaccaaagc cgcgtctcag gcctacaacg aactttctgc gggccaaaat agccctctaa
      721 tcaaccgcgc cacctcgcag ctgtacccac cagggtccac gttcaagacc atcgtcgcct
      781 cggctgcgct ggaaaccggc gattatcaga cggacacgaa gattcctgcg ggcgccagct
      841 ataccttgcc gggaaccgcc acacagttgc cgaacgccga ggcacaggcc gatggcgtca
      901 acgggcagat ctccctgcaa gaggccgtcg catggtcgtc caataccgcg ttcgcccagc
      961 ttggcgtgaa actcggcgcg agcaaggttt cggacatggc caccaagctt ggtttcggca
     1021 gcaccatcac cattgacggc accgaatcca cgggcacccc gatgacggcc accgcgtcca
     1081 cattcccctc tgatccgacc gacgacaagc ttgcgctggc gtccatcggc caaggcgaca
     1141 ccacggccac gccgttgcag atggcgatgg tcgcgcaggc aatcgccaac aacggcaaac
     1201 tgatgcagcc cacgctggtc gatcgtgtgc gcgccgccga tctcaccgtg ctttcgcaga
     1261 ccaagccgca gaccatggcc aatgcgttca gcaaggattc cgcagacaag ctcaccacga
     1321 tgatggaaag cgtggtgtcc gaggccaatc cgcagctggc catcgacggc atcaaggtgg
     1381 cggccaaaac cggtaccgca caaatcggta ccgacaactc tgccatcgac ggctgggtga
     1441 tcggtttcgc gccggcggac gatccgcaaa tcgccgtcgc ggtgttggtg cataacacag
     1501 atgtatacgg ttcgctcgcc gctggcccga tcatgcgcgc gatgatgcag gaggctttgc
     1561 agcagtgaaa cttatcgaag gacagcttat tcaccgccga tatcgtcttg attcgcgtct
     1621 tgcgcaaggc ggtatgggcg aggtctggaa aggctacgat attcagctcg gccgtgaagt
     1681 ggccatcaag gcgctgcgct cggacatgac caacgccgag gccaagctac gccgtctgcg
     1741 tgccgaagcc cataattccg ccaatctggc gcatccgaat atcgctgcgt tgttcgaata
     1801 ctacgagcat gacggcatcg gctttctgat tatggaatac gtcccctcga aatcgttggc
     1861 ggacctgttc cacagcaagg gcgcgatgga tcccatcgaa ctgctgccca tcctgattca
     1921 gaccgcgcga ggcctgttcg tggcccactc gcacggcgtg attcatcgtg acgtcaagcc
     1981 ggccaacatc atggtttccg acaccggcga agtgaaaatc accgatttcg gtgtgagcta
     2041 ctccacgggt cagggccaga tcacccagga cggcatggtg gtgggcaccg cacaatacat
     2101 ctcccccgag caggcgcagg gccagcaggc cacgccgcaa tcggacatct attcactggg
     2161 cgtagtggcg tacgagggcc tttccgggca tcgtccgttc accggcacca ctccggtcga
     2221 tatcgccgca gcgcacgtga acaatcccgt gcctccgctg ccggactcgg tggacgtcca
     2281 gttacgcgaa tttgtcatgt caatgctggc caaggacccg ttggatcgcc cgaaagacgc
     2341 gctggtggtg tcccgtacgc tggcacgcat cgaacgccgt ctgctggatc agcagaccga
     2401 aatggccgac accgccatgg ccacgtccgg caatcgactg ccgcgccgag tgaccagcac
     2461 gccacgcatt gttttggagg ccccggcctc aagattggga gggaataagc aatgagcacc
     2521 agcatgccca ccgcattggc cggcggacgc taccagcttg gccagctcat cggtcgtggc
     2581 ggcatggccg aggtccacgt ggctcttgac acccgtctgg gccgtaccgt ggccgtgaaa
     2641 atcatgcgcg ccgatctggc caacgacgac atcttcctgg cccgtttccg ccgcgaagca
     2701 cacgccgtgg cccagatgaa caacccgaac atcgtgaaca tctacgattc gggagaagaa
     2761 ctggtttctt ccgaaagcgg cgatgccgag cgtctgccat acatcgtcat ggaatacgtc
     2821 aagggccaga ccctgcgcga catcatcaag gtcaacggcg cactcagcca gcgcgactgc
     2881 gaacaggtga tgttgggcgt gcttaacgcg ctcgactatt cgcatcgcat gggcattatc
     2941 caccgcgata tcaagcccgg caacatcatg atttccgagc agggcgtggt caaggtcatg
     3001 gacttcggca tcgcccgcgc tctggacgat tccgcagcca ccatgaccca gtcgcagggc
     3061 gtggtgggca ccgcacagta cctttctccc gagcaggcgc gcggcgagac cgtcgacatg
     3121 cgttccgacc tgtattccgc cggctgcgtg ctctacgaga tgctcaccgg ccgcccgccg
     3181 tttaccggcg attccgccgt ggccatcgcc taccagcatg tttccgaagt cgccacgccg
     3241 ccatccgccg tggtgccggg gctgccgaag atgtgggatt cgatctgcgc caaggccatg
     3301 gccaaggacc gccagaatcg ctatgccacg gcctccgaat tcaagaccga catcctgacc
     3361 tacatgaacg gcggcgtgcc ggtggcggcc gcgttcaacc cgttgacgga cctgtcgaat
     3421 atgaaggccc gcaaggaagc cgaacgtgat ttgcccacca cgccggtgga accgcatcag
     3481 caacccaccc aagcattcaa cccggtgacc ggtcagttcg agcagattcc accggccaat
     3541 ggtgccaact cagcggcgtt gcagtcccgt gcgcagcagc gtgcggcggc ggccaaggcc
     3601 aagaagcgca agaagatcat catcggttcg gtgatcgcag cgattgttgc agtgcttgtc
     3661 attgtgggca tcgtgttcgc catgaacggc tccggcaaca agtcctcgga agacaccgtc
     3721 accatccctg aggtgtgcaa cgcttccaca tccaaggaca gcatcgagct caaactcaag
     3781 gcatcgggtt tgaagatgac cgaaaagcaa gacaccgact ccaccgagcc ggaaggcacc
     3841 tgcaccaaga tgagcccgga cgccggctcc aaggtggcca agggatctgc cgtgaaggtg
     3901 tggttctccg caggcccgca gtccactcag gttccggatg tcaaggaacg cagccaggaa
     3961 gaggcgcgca gcattctgga aagcgccggg ttcaaggtga acgccgccgt caaaaccgaa
     4021 gacagcgccg acatcgccaa ggatatggtg acgaaaaccg atcctgccgc cggtcagtcg
     4081 gttcccaagg gcaccaccat caccatatat gtttcttccg gcatgaccac cgtaccgagc
     4141 aatttggtcg gtcagtcgaa ggacagcgtg ctacagcagt atgagggcaa gttcagcttc
     4201 actgtggagc aggagagctc cgatacggtg gaggccggtc tgattacccg tgtaagccct
     4261 gattcgggct cttccatcgc acagggcggg ttcatcacca tttgggtgtc cacgggcaag
     4321 gagaaggtcg ctgtgccgaa tatcaccgct ggcactgact acgtcaccgc cgaactcatg
     4381 ctcaaggccg tgggcctcaa ggctcaggcc aatggtccaa ccggcagcac cgctgtggta
     4441 gtcagcatca atcctggcgc tggcagccag gtagatgcgg gatcgaccgt caccattacc
     4501 acgaaggccg gcagcactgg cggcggtacc ggtaccggag acggtggcaa cggtactggc
     4561 aacgggggcg gaaccggtga gtgaaccggt gagtagcaag taagcgccgg gcgtgtatgt
     4621 gttgcgcata tgcaacgcaa atagcgcaac acatacacgc aacacaaagg ggaagggctt
     4681 ctgcgcccct cccctttgct gtataaagag ctatcccagc gcaacaatcc cgggaagcgt
     4741 cgcaaaacca gtacgcaagc ctcgttctag cgacacattc cggggagagt atcgccgcgc
     4801 ggaaaagcta cttcgtaact ttgggctgga ggccggcgga cttggccacg gcgttgtctt
     4861 ggccgcagac cttaagccag ttggccagca gacggtagcc gtcctgggtc atcacggatt
     4921 cgggatggaa ttgcacggcg tacatcggct tgcccttgac acgcacgccc tgaataatgt
     4981 ggtcgccttg cgtccacgcg gtaaccacca gcgtatccgg caccgaatcg ggctcgacgg
     5041 ccaacgaatg gtagcgggtg gcggtcatgg ggttggccac gccctcgaaa atctcgtcat
     5101 cgatatgttc caccaagctg gtcttgccat gcatgatggt gggcgcatgg tccacagtgc
     5161 agccgtacac ctcagcgagc gcctgcaagc ccaggcacac accaaacatc ggcacgccgc
     5221 tggccgcgca caggcgaatc atatcctcac tggcaccgga ttcggccgga gcaccagggc
     5281 cgggagaaat cagcacgcca tcgtaaccgt ccagtacacc cggcgcggct gggtcgatag
     5341 cgtcgttacg caccacatca acagtggcgc caagggtttg cagatagcct acgatcgtgt
     5401 agacgaagga atcgtagtta tccaccacca aaatatgcgc ggaatcggtc atagggctct
     5461 ccttgaggga cgtgtactgc aagccatagt actaggatgc cgtcagctcg ctgccacgaa
     5521 gttcgacatc tgctgtagga atgggatatt ggcggcggcc caagggaatc cccactggaa
     5581 cagcgccgcg gcggctgcga acagcagcag agccagcagc aaccatcgaa tcggtgccac
     5641 gccgggctgc agacgcaaca gtccgccata gatgctggca tccggacggc gacgctcacc
     5701 cgcgcgaatc tcgcgcagca ccggccagcg ccatgccacc gcagcggcaa tgaacagaac
     5761 cagccacact accagcgcgc cgaataccac cttgtccagc gagccaatgc gggaagcgat
     5821 cgagggggtt tcggtggtgg agaacatcac tgcaccggac gaatcagtag tagccagctc
     5881 tttgggcaca ccgtcggaca ctttggccca gtacgccaac tcaccatagc tgatccatcg
     5941 atgggtgggg gtggagtatt tcggctcaca ggtggtcagt gtgatcatgc gtttggtggg
     6001 attggcagtg gagtcttccg gattgggggc gatcacatgc acgtccgtgg gcagcacaat
     6061 ctcgtaccgg gtgtagtggt agacgtacca gtaatccttg gtacgaacga tgatgggatc
     6121 gccttcctgc aacttgtcca catcgcccaa cggctgcccg tagccgttgc ggtggccggc
     6181 gacggcgaag ttgccgacct gccccggcat ctgggtggtg tcgtaatggc ccagaccgtg
     6241 gcgattgagc tgctcgagcg tggtgccttc cacgatgttg cggtgccatt gggagccgaa
     6301 gcgcggaatg tagatctgcg caatcaagtc gccatatttc gcgtctttcg gctgtaccgg
     6361 cgcatcgccc tcctgggctt tggcgatggt gaccgttccc ccgttgttgc tggggtcgct
     6421 ccagtcgacg gactgggtgg tttcgttctg ggctcgctcc gcctcgacac cggtccacca
     6481 catctgccag acgatgtaca gggcgcaaat cacggcagcg gtaatcagca gttcggcgca
     6541 gatgccgagt gcctgccaga ccgggctccg ctggggcgaa gacgcgccgc gtactgcggc
     6601 acgacgggac cttgccctac tggcgttggc cgggtctgcc gccattcgtg cggcatcgtc
     6661 ctcggggaag gggtgtacgt ccgacacgga cgcgagatca atgggcgccg gtacgccgct
     6721 gttgtgcctg ccgtgtctca ttgctgagtc tgctcctcat ctgtggtctt tgacgtatcg
     6781 accgtggcat atttcaactg ctgcagcaag gcggcggtag cggggaactg aaggtcctcg
     6841 gcattctcca ccttgtagcc aatgccaaac gcggtcacat attgcttaag aatggtaatg
     6901 gcttcggagt cgttcaacgc tgcgcgaagt ctttgagggt caccaatcgc ggaaatggtg
     6961 aacggcggcg agtatttctt accgtgcaac gacagcacgt tgccggaaca gatgaccgcc
     7021 gaattgaaca gcacccgctg atccataatc atcagcgcat cggcaccgcc ggcccacaag
     7081 gcattcacca ccgattcgac gtcctgctgg tggatcacgt aatcgttgat attggcggtc
     7141 gtgccgttgt tgttgactgc gttctcccac aacggagaat catccagcgt taccgttacg
     7201 ccgctgccgc tgagcgcggg cagcatcagg ctggaatcca cattgctgga gtccttgcct
     7261 tggttctgtg tgctgtcggt gccatcggcc ttgagcgcgt ccgtaagatt ggcgacctgt
     7321 tcggtcagat cgctgacgtc ctgctgcagc tggttgacct cgtccacgcc ttgctccacc
     7381 agatcagccg tatcgttggt gacggcaacc gtacgattga ccctcacgtt ggtgctcagc
     7441 agaaaaccgg tcaaggcaat gacgacgaat accgcgacac caccccatag ggagtgccgt
     7501 gcaccgtgct tacctacatg tctggccatg aatttcctgt cttcttgccg actctggtcg
     7561 ccgctttcga gtgtagccac tattatgact tttagcctat tgaatggaga ctatgctgat
     7621 ggctgacgaa gagctgcacg aaaccgactc gaacaaccag aaggactggc agagcgagac
     7681 ctcatccagc accgttgctg acgagaccgc cagcaaagac accgagcaga acgcagagaa
     7741 cgcttctgcc gaggaaggcg cggcttacgg cgtgaatatg gagcaggtgc aggccgtgct
     7801 caacgccact gccgataagg ccacgctgac cccgcagatg cagcgtatga tgaaccgcca
     7861 ggcggagaac accaagcgcg ttgaagaaac cattaagggc accaagtcga acccgcgttg
     7921 gttcgtgccg ctgttctgcg cgtttatgat catcggcctg atttggtgcg tggtgtacta
     7981 cctctccccg tccggttcct ggccgattcc gaacatcggt gcgtggaacc tcggcatcgg
     8041 cttcgccctg atcatgatcg gattcctgat gacgatggga tggcgctgag ttccggctcg
     8101 ttgcgcttat ctatcggtag attgtttttt ctatcgttag gttgtcctta attcttatga
     8161 gaaaggccga cctcccgtat gcggaggtcg gcctttttcg tgccttagcc tgttcatcca
     8221 cagctatcca ccacttatcc acgacttatc cactacgtta tccacagcct gtggatgaat
     8281 tacacccatg tagttttcca ccgaatgtgg aaaaccatgt ggataagtgg tggataagcg
     8341 gcgtttctcg gtgtatatct cgtgcataac tttggcttgg catacatcgc cacagcgagg
     8401 gaacagttat acacccgagt tatccactgc aacaaaccca ataataccgc ggcattccta
     8461 acttatccac ataagttatc cacagtctta tgcacttttc tacacacttg tgcacaagtc
     8521 accattgtgg ataactcatt atcgtggata actcgtcatt gcgcgcatct cattactgtg
     8581 gatatcttca tacggcacac acccactcgc cgaacgacga tatccacatc gccgctttcc
     8641 gcacatacac tgatgccggg cactgcatgt tccgcaatac ccggcatcaa tcgttttttt
     8701 ttacagccat atcaagccat atcagtgcag ggagccgaaa ctcatccacc cgtaaggatt
     8761 ggccatattg cacaacagaa tcaacgcaat gaccagcacc accatggccc agccgtacac
     8821 ctgcatgcgc catttcaggc ttttaccgcg ccacgccggc acaccaccca ccagcagcca
     8881 tgtgagtatt ccacccacta caaagccgcc gacatgcgcc tgccacgcca cgccaccgac
     8941 cacgaacggc aaggcgaagt tcaccgccat ccagatcaac atggagcgga tatcggcgcc
     9001 gatacggcga tataccacca gcaatgatgc aaacaggcca aataaggctc cagaagcccc
     9061 ataggctgcc atctgccagc cgatgcctcc cggagccacc gcagcccaca ccataagtcc
     9121 cagggagccg ccaaggccgg aaatcacata cagtcccaag aaccgccagt ggccgatcat
     9181 tcgttccagc actgggccca cggaatacag agcaatcatg ttgaacagga tatgcaatag
     9241 cgagttcggc gcatgcagga acatcgaggt gatgagcgtc cacggctcag ccaccagtct
     9301 gaccggagcc gccatgccga ggtacatcaa ccaagccctc aggcccgggg caataaatcc
     9361 caacaatacc tccagtagcc acactgccac gcacgcaaca atcagcgccc aggtcatcac
     9421 cggaccaccg cgctcccact ggtatcgaat ctcccgttgt gtaggaatat gcatgcagcc
     9481 cactgtatcg aaggtctcgg atgcgttcca cgcgccgcga agatgccgag ccggccaagg
     9541 gcagacgggc gcggatgaat cgcccacgaa tgtcattcca cccaatcgtc acaaacagcg
     9601 gcctagaata gggagcagag tcggtctgaa tgggcatacc gaccagacga tccctaggga
     9661 aaggagccgt catggagaac aagtcttcta acggttggaa gttcttcgcg ctgctgttcg
     9721 gcggattgct cgccgcgctt gttggcctgt acatctacaa gcagcagaac ccggattacg
     9781 atccgtggga ggagccgtgg gagaacagct cctcgccggt cgacctaggt ctgaataagg
     9841 ccgaggatga ggccgatgac gaggcgaaag ccgccccggc cgaagcctga tagggcggtc
     9901 tttcaacgca agaagccccg caaccgatgg atgtcggttg cggggcttct tatgctccgg
     9961 cggccgtcag gccagaggag cagtgtgcca gatacgcgga gccgttaagc gggaagcccg
    10021 gtgggcttcc cgtaggcgac gccgagcggc gacagccgcg aggtagaaac cagcacgtag
    10081 ctggcgcgcc gctccggcgg ccgtcaggcc agaggagcag tgtgccagat acgttccaaa
    10141 tagtcttcca tggagcggtc ggagctgaag tagccggagt tggccacgtt gagcagggcc
    10201 ttgcggttcc attcgagctc atcagcgtag agggcctcga tctcggactg gatgtccatg
    10261 taggcggaga agtcggccaa ggtcatgaac cagtccttgg tcagccagtc ggccacgagc
    10321 ggggagtagg cgttgcggtc gccgttggag aaggtgccgt cggccaccat atcgatggcg
    10381 tgcttcaggc gcgggtcggc ctcgtagtag gaggcgggat tgtagccctc ggcgtacttc
    10441 ttctcgacct cgtcgacggt catgccgaac aggaagaagt tctcggcgcc gacgcgctca
    10501 cggatctcga cgttcgcgcc atcgagcgta ccgacggtca tcgcgccgtt gagggcgaac
    10561 ttcatgttgc cggtacccga ggcctccttg ccggcctgcg agatctgctc gtcgaggtcg
    10621 gtggcgggaa tcaggtgctg ggcgaggcgc acattgtagt tccacgggaa gtacacggcc
    10681 agcttgccct tgacgtccgg atcgttgttg accacgcggg agacgttgtt gatgagctgg
    10741 atggtcatct tggccaggta gtagcccggg gcggccttgg caccgaagaa cacggtgcgc
    10801 ggggtgacgt cctcggcctt gaccttgccg gacttgatgt cggcgtaggt ggagatgacg
    10861 gacaggatct tcagggactg gcgcttgtac tcgtgcaggc gcttaaccat ggtgttgaac
    10921 atggtgttcg ggtcgagctc gaagccgtac tcgcgcttgg cgtaggcggc gaagtcgacc
    10981 ttgttggcct tcttgacggc ggcgaacttc ttgacgaatt catcgtcctt ggccagcggc
    11041 tcgaggccct tgagcatctc caggtcggag agccacttgt cggtgccgag gccctcggtg
    11101 atgaggtcgg acaggcgcgg gttggcgagc ttgacgaagc gacgcggagt cacgccgttg
    11161 gtcacgttgg tgaacttgtc cgggtagacg tcggagaagt tcttgagggt gacgtccttc
    11221 aggagctggg agtgcagctc ggccacgccg ttgacgtggg agccgccgta ggtggccaag
    11281 taggccatgc gcacggcgtc gccagtgtag atggccatgt ccttgatggt gttcttgtcg
    11341 acgcccttgg acttgagctc gtcctcgaac tgcgcgttga tcttctcgat gatctcaagg
    11401 tgacgcggca gcagctcgcc gatcaggctg gccggccaga cctcaagggc ctccggcagc
    11461 agggtgtggc aggtgtagtt gaaggtcttg gtggtgatgg accacgcggt atcccagtcg
    11521 tagccgtact catcgatgag gatgcgcatg agctcgggga tgccgatcac cgggtgggtg
    11581 tcgttgagct ggaagacgat cttgttcggg aaagtcgtca gatccggctt gtcctggccc
    11641 gggtagaaga cgcggatggc gtcgtgcagg gaggcggaga cgaagaagta ctgctgctca
    11701 aggcgcagtt ccttgccgac cttggtggag tcttccgggt agagaatctt ggagatgttc
    11761 tcggccttga cctgcggggt cacggcttcc atgtactcgg agcggttgaa ggcgagcaga
    11821 tcgaactcgt cgtagctctt ggcgctccac aggcgcaggg tgttcacgcg gccggacttg
    11881 tagccgggga ccaggtagtc caccggcacg gcgcgcacgg accatgcggg ctgccaggtc
    11941 ttggtgccgt cggcgttctc gacgaccttg ccgccgaagg aaaccttctg gtcgcggttg
    12001 tagtcgatgt ggccccacgg ctcttcgttg gccagccagt agtccggggt ttcgacctgc
    12061 ttgccgtcct tgtcgaactc ctgcttgaag atgccgtact tgtactggat gccgtagccg
    12121 aaggccggca cgcccagcga ggcgagggag tcgatgaagc aggcggccag acggccgagg
    12181 ccgccgttgc cgaggcccgg ctcgtattcg gcgtcgacga cgtcctggac cttgaagccg
    12241 agggcttcca cggccttgtc gaactgctcg gtcagaccgg cgttgagcag cgcgttgcgc
    12301 agctgcttac ccatcaggaa ttctgcggac aggtagccga cggccttggt gttgccgttc
    12361 acggtgtcgg cctgggtctt gaaccaggag tcggcaaggt ggttgcgcac ggccttggat
    12421 gcggcgacat acacgtcagc aggggtagcc tgctcggtgg tgacgttctg agtgtatttc
    12481 agttgttcac gaatctcgtc ggcgaattgt tccgccgtga caggagactt cggtgcggtg
    12541 atttcggtca ttctgacttt ccttccggga taagtgcccg tgtttattat gccgcgcgat
    12601 aggtatcgtc gcacaaacgt aagcatgaac tctccgtaac gacgatggcg gagcgttctt
    12661 gcatacgttc tcatttagag cgtagcacat gccccgatat tccgcatcac accgtatgcc
    12721 atcgtttgca ttatggaaga tcttccggct ccggtgccat gatgggatac atggcagtaa
    12781 ttcttgaaaa agacctgaaa cgcgggcatg acgcgctggt gcaatggcgt gccgcggccc
    12841 gggagctggc ggacaacaca ccacatcgtc tgaccgtctc ccctgtcttg ccacgcccgg
    12901 tgtgggcgat ggccgtgcgc tactcgctgt ttctgctgga gcggcgcgca cccggccctg
    12961 gcgtggaggt gcgtgtcgcg ccgtgggggg cggtcaaaat cttggacggc ccggaatcgg
    13021 acccgcataa cctgaccccg ccggacgtca tcgagctgga cccggaagtc tggctgcggc
    13081 tggcctgcgg catcaccaca tgggcagagg aaaaggacgc cggccacatc agcgcggtcg
    13141 gcgagcgcga cgacctgagc gacctgctgc cgctgtgagg cgcgaagcgt aagtaagcca
    13201 ccagcggcgg caccatcagc cataaccaca aatccccttg tgctcaacca agcacaaggg
    13261 gattattacg agaagaccgg cgttcagctc ttcttgggct tgagcgcgga aggcatgggc
    13321 atcggaatcg gcttgcgcgg ggccggcttc ggtgcggcag gcgcggaggc cgggaactcg
    13381 ccgctggtcg cctttgcggc ggcttcggca gcccacttgg cgtactcgtc gaggtcatcc
    13441 tcctgcgtag cgggagctgc ctcggcggca ccggaagcgt ggtcatcagc agcggaatcc
    13501 acatccttgt ccgcatactg gtcatcgatg tcggcgaacg ggtcaggaga gcccattccc
    13561 ggctcctgct cgctcagctc ttttgccagc tcatcgtagt cggtatcggt ggtcaggtac
    13621 ttgagctttc gggcaatttt ctgctgtttt gctttttgac gtccgcggcc catctgaccc
    13681 cctgcgatta ctgtgctgtt cgattcatca acaatagaga gtaccactaa tagtggcttg
    13741 ttcgttgtcg gctcacactt ttaccataaa aagcgtttat ttagggcata ttcaaaggat
    13801 tttcgcatga ccgacgagca gcaactgaca gcggcaggca atgagatgag cgcgagcttc
    13861 ctcgcggcca aaaagcgttc ggacgaaacg ctcgccaagc tggaggccaa gccgtcgtcc
    13921 ttcaccatgt tgaccggcga ccgcccgacc ggccggctgc acctcggcca ctacttcggc
    13981 tcgatccgtg agcgtgtcgc catgcaggag cgcggcgtca acacgaacat catcatcgcc
    14041 gactaccagg tgatcaccga ccgcgacacc accgagcaca tccaagacaa cgtgctgaac
    14101 ctggtgctcg actacatggc cgccggcatc gatccgaaca agaccatgat gttcacccac
    14161 tccgccgtgc cggccgagaa ccagctactg ctgccgttcc tgtccttggt caccgaggcc
    14221 gagctgcacc gcaacccgac cgtgaagtcc gagatggagg cgtccggcca cgcgctgacc
    14281 ggtctgctgc tcacctaccc ggtgcatcag gcgtgcgaca tcctcttctg caaggccaat
    14341 gtcgtgccga tcggcaagga caacctgccg cacgtggaaa tcacgcgcac catcgcccgc
    14401 cgtttcaacg agcgttacgc caagaagaac ccggtgttcc cggagccggc cgccattctg
    14461 tctgaggcgc cggagattcc gggtctcgac ggccgcaaga tgagcaagtc gtacggcaac
    14521 tccatcatgc tgggcgccac cgccgaggaa accgccaagc tcatcaagaa gagtccgacc
    14581 gattccgagc gccgcatcac cttcgacccg gttgcccgcc cgcaggtctc cgccctgctg
    14641 accaccgccg gcctggtgac cggccgcgac ccgaaggaca tcgccgagga gatcggtgat
    14701 tccggcgccg gcgcgctcaa ggcatacgtc atcgaatcag tcaacgagtt cctggccccg
    14761 caccgcgagc gccgcgccga gctggccaag gacatggatt ccattcgtga catcctgcac
    14821 gacggcaaca agcgcgccaa cgccatcgcc gaggagaccc tcgaccaggt gcgcgaggcc
    14881 atgggcatga agtactgatt ttcccgccgc tcgtagcaag acgccgtgtc agcccccaac
    14941 tgggcaactg acacggcgtt tttgtattgc ggcgtctgct gtgtcctatc ttcacttcga
    15001 ggggcggatt tcttgttttt ccgtgcaacg cggctatcaa gacggacctc tgttcacttc
    15061 atgccgatgg cttgcatcaa tacgtacgct ccccgcacag caatgtcatt acgacgtgtt
    15121 gcggcatagc gcgcatggct tccggagtat cggcggcgta tgggtcggcg tccagcaaca
    15181 ccacatcggc cgggtcgccg gatgcaaggc ggtcacggcc agtggcggtc gaggctgcca
    15241 gcgaggtatg cacatcgagg cactgctcgg gctggaacgg ctcgcggtca gagctttcgg
    15301 tgccgagcac ggcggcggaa atggccagcc acgggtccag cggggccacg ggcgcatccg
    15361 agcccatgcg cagcgtggta ccggcgtcgt ggagggccct gaacggataa gggatgccgg
    15421 ccgggttggc ccagaaacgg gtgatgacgt cgcggtcatc catggcgtgc tgcggctgga
    15481 tgctggccgt gagccccagc tcggcgaaac gcgggatatc gacgggtttg agcatctgcg
    15541 catgctcgat cgatccatgt gcgtgcgtgg cggcgactgc gttgagcgca atggtgttcg
    15601 cttcgtcgcc aatggcgtgg caggcaatgt cgaagccatg ctcggtggct aggcgcatat
    15661 agtcttcgat ttgctgcggc gtgtagctca gcgtgccata cgtcagcggc tcgatgcccg
    15721 aatacggcgt ggagcagtag gcggagcggg tgttgagcga tccgtccgag atgagcttca
    15781 tggcgcccac atggccaagc ccggcgctgc ccggcaattc cttgccggta tgccaacccg
    15841 cgtcaatggc ggctcgcaac cgctcggggt atacaccggc gtccacgcgc aaaccgttaa
    15901 tgccggccgc gaaacgattg atccatgtat cgatgttttc ggccatttca taatcgcgga
    15961 tgcccaccac gcccttgctt gcggcgtcct gttcggcctc gcgcagcaga cggtcagtct
    16021 cttcagcagc gcccggcgcc ttgtcgaagg cggtgtaggc gttgaaccat tcgagttcac
    16081 cgaccagacc ggattcgtcc acatgtacgc caagcctgcg ggccgcggcg gaattcaccc
    16141 atccgcagtg catgtccgcg ctggagaggg ccaccggctg ttcgccggtc acggcgtcaa
    16201 ttgcggccag tgtgggctgc tcgtcgtcgg cccacagcga gtgacggaat cgcatgccca
    16261 ccacgaaggc atctgggtca agcgtgtcgg cggcgcggcg ctcatccaga tgacggcgca
    16321 acatgtccat cgcttcggcg gcggaacggg cattgatgag gtccagtcgg cccagcgtct
    16381 tagcccattg cgtgaagtgc gtgtggcagt cccacaggcc gggaataacc cacaggccgt
    16441 cggcgtcgat ggtggtttcg tcgacgggtg cggcggccga cgttgggatt gagacgctgc
    16501 cagaagcaat ggcattcgtc gcgtattcca acgatgtcgt atgcgcctta gtctgcatcg
    16561 tgctgtccga agccgagacc gttccagcag ggccgacaga cgcaacaccc ccatcgcgaa
    16621 gcaccacatc cacgcggtcc tgagaccccg gaacaccggc attgacaata cgcaccaagc
    16681 tactcatacc ggccatcatg ccacctttgc atgaacgcca tggagattcg tccaagttga
    16741 atcgcgccgt cgacggcgat tcccgctggc tgcctgcaaa cccctacgtt ccatgtaatt
    16801 gcgggatggc ctgcaactgc gatgaacacc catagtttat ccaccgactt atccacattt
    16861 atccaccgaa aaattggggc ggtagacagg gccggtagcc ggactctaca ctgcgaggac
    16921 aaagcgaaca tcacgaaggg gtgatcatca tgggtagctc aacggcattg accgagctga
    16981 caatgcagaa gtaccaacga atgcagacgt gcgcagatgt tcaacgacga agcgcatggc
    17041 gcccacccta tgccctgaca acagcgttgg agcttttgtc aattgaagta ccccgcatat
    17101 caagcaaaca cagaggcctt gcaactacaa ccattgttgc ggttccacac gccaatgaca
    17161 aacggcacat tgtgggggtc aaagtggttg tatggccttt tccactagac acggtcataa
    17221 tcgagggaca attcacatgc acttcaccag cttgcacatg ggccatgttc tccacgtatc
    17281 tcgaacttga agaactcatc gttctcgctg attccatgat gcgacgagat cgaaggctct
    17341 gcagaacaac catcgacgcc ctttctctgt atctcgatga ggccgaggcc caggtgcgag
    17401 ccgacaaaga gaatggcacc aattcgtatt tgttccgcgg gtataacaaa tgccgtcgcg
    17461 ccctactttt ggcgcgcgcg ggcacagatt cctccatgga aacacgaact cgtttggttc
    17521 tcttgaaata cggtctgaat tgcccgcagg tgaattatcc aatattcgta ggaaacaaca
    17581 ccagacccat atatctcgat cttgcatacc ctgaattcaa gatatgtatc gaatacgaag
    17641 gctctcatca tgccgggcaa tggctgaatg acgcgagacg ccgccagatg atcgaagatg
    17701 ctggctggaa gtacattcaa gtcaccaaat tagatattgg cgacgaagcg ggagaagagg
    17761 cccttgcccg aagagttgcc gaaaggatac aggaggttac gggcaaaacg gtccagctca
    17821 cgatgcggca aactacccag caaattagcg atgtccgaaa gctacgccgt attccactgt
    17881 acaaacggct gaaattcgag cctctgctgc caattattcc gacgacgcag gaataataag
    17941 cccggtagag tcagtccgca ccgtccgcac cgcgctacgg tattcaaaac ccatttcaag
    18001 gggctgattt ggcctccgcc ggaggtctac ttccgtttca aggggctgat catccggtag
    18061 accacgtgta tcaatgccct tacgtcaacg ccattccgcc gttttggagc cgcgtctact
    18121 caggcatcag ccccttgaaa cggaagtaga tctctccaga acgtcaaatc agcccctcga
    18181 agtccgtttt ggcctcaatg gttcctgccg aaccacaaaa aagccactcc tctgccatac
    18241 gagccgaccg cacagcaggc acgtgtcatt tgccgctgcg gacctcggac tccatggctt
    18301 ccatgggctc gtcgacgagc acggtgccgg acggcagggt gccgggcagc ggatcgtcca
    18361 tgtagtggtc gaattcgtag agcatgcgct tgttcggctt ggcggtcagc aacgaaacca
    18421 ccacgatgaa cagcagcgaa atgatgaacg ccggcagcag ctcatagatg gcgaatacgc
    18481 cgcccaaggg cttgacgagg ttgtgccaga tcagcacggc cgccgtgccg gacagcatgc
    18541 cggccacggc gccggccttg ttggcgcgac gccagtatag cgagcacagc atcaacgggc
    18601 cgaatgaggc accaagcccg gcccacgcgt aagacaccac ctggaagatc gacgagttct
    18661 ggtcgtatgc caccagaatg ccgaacagga acatgaccac cagcgtgacc ctggccacaa
    18721 tcatgacctg cttgtcggtg gccttgcggt gcagcagccc gcggaagatg ttctcgccca
    18781 tggccgaagc gccgatgatc agatacgagg agctggagct catcgaggcc gcgaaaatgc
    18841 cggagaccac cacgccgcac atgaagctcg gcagcagggt ctgcgccagc acgatgaaga
    18901 tggtttccgc atcggtggag gtcagcagtt cggtgggcat catcgcacgc cccaccaagc
    18961 cgatgcacac tgcgcaaccc agcgaaatca cgcaccagga ggtggcgatg attcgggact
    19021 tgcgcacttc ttccacgctg cggatggaca ggaagcgcac cagcacctgc ggcataccga
    19081 agtaacccag gccccacgcc agcatggaga tgatggtgat gatgccgtag tcggagggcg
    19141 cgccgaacac ggctttgccc gcctcgacga gctgctggcc ggtggccggg tccagcttgg
    19201 gcgcggccac ctgcgtgccg gacaggtagc cggggatggc acgcaggaat tccacggtgt
    19261 tgtcgatgcc gcccgcgctg gccacggtgc cgatgaacac caccgcgagc gcgaagaaca
    19321 tgagcatgcc ttgaatgaag tcggtcatca ccacggacaa gtagccgccc accacggtgt
    19381 agacgaacac gaccaccgcg ccgatgatca tcatgacgtg gtagtcgaaa ccgaacaacg
    19441 tggcgaacag cttgcccacg gtcacgaagc agctgcccac gtacacgctg aagaacacca
    19501 ggatgatcac ggccgcgatg gtggagacca cgcccttctt gtcatggaaa cgcttggcga
    19561 agaagtcagg aatggtgatg gcatcgccgg ccactaccga gtagcggcgc agtcgccggg
    19621 ccacaagctt ccagttgaga taggtgccga gcgccaggcc gatggcggtc cacatcgggt
    19681 cggccgcgcc ggtgaagtag gccaggcccg gcaggcccat cagcagccag ccgctcatgt
    19741 cggaggcttc ggcgctcagg gccgtgagcc acggccccac gccgcgcccg cccgcaaagt
    19801 attccttgga ggaggagttg gagcgtttgg agtagatgaa tccgatggac agcatggccg
    19861 caaaatagat gaccatggcc agcagaatcc agaaatcgtt ggccaactgc gacacctccc
    19921 caatcatgac gccaagcggc taccgccatg gcagaactat gtgctgatat gcgtgctgat
    19981 atgcgattga cgccggagcg gcgtcataca gaccgtcaca gccgtgaata cggccgcaac
    20041 gcacttcgcc tgatggtagt gaatacaagg gctcaagcac cttccaccgt ttccatactg
    20101 agacgctgtt ttatccgagc tcccctcacc tgaggggagc caagatgacg gtcagatgtc
    20161 gtagcgccag gacttgtcgg tcaccacgcg ggccatcgcc aaaccgatcg gcagacagat
    20221 aatcaccatg aacgcacgga acgcccagtc cagcgcattc atggtctgga agctgccgag
    20281 gtaccacatg gcacggtaca tatacagacc gggcaccatg atcacgatcg acggcacggt
    20341 caggcagatg cgcgggtagc cgaggtgcgg cggtagccag ccacgtcgca cggatgaacg
    20401 ccacgccgat gccagaatgc ccgccaggaa cgcgccgatg aaggtagcca tctcgggtgc
    20461 cacgttgaag tcttgcaatt ccaagcgcag cgtgtccgtg atcgcaccga tcagggcggc
    20521 ggccaggcac atgcgctgcg gcgaattgaa cagcacggag aagccccaca cgccgacgaa
    20581 cgccgccacg aaccgcagca atccgttgac ccacgggtca agccccagcg gttcaaagcc
    20641 ttccgggtgc aggtgcacca tcacggccac ggcccagcct gccagcgtgg ccatcaggat
    20701 gatgcacagc acgtacgcca cgcgctggat gcccgagggg aagtcgattt tcgccatgtc
    20761 gagtccgccg gtgatgagcg ggaaaccggg gatcacgaac agcatggcgc caatgtaggc
    20821 cgtatcgtgc gagagcgcga ctgggtcgaa aatgccgatg agtcgcaggg tgccggtgca
    20881 ggcgagcgcc gccgcggcca cgcacacaaa ggtgacgaag aactggttca gatggtatgc
    20941 aaacagccgg cgacgcatcc actggccaag gcccgcgccc acgaacgcac cgatcatgtc
    21001 gtatgggcca ccgccgagca ggaacacgaa ggccgcgcag gccagggcgg aggccagacc
    21061 ggagaaccac ggcttgtaca gcggcttgcg gcgctcaatc agatcgagtc gctcgtgcgc
    21121 ctgccgcacg gtgataccgc ccttggcctt ctcttcgtcg gagcgctcgt tgaagtgttc
    21181 ggcgtattcc ttgggcacgc gattcttgcg cttcgtactg cttgcagcag ggctttcggc
    21241 ggttgcgccg ttcgccgccg cgccctcagc gcccgcctcg gcattcgccg cgttcgccga
    21301 ttcaaccgac tcattggagt caccggtaaa cacttcggca tcgtcgtagg ccttctcttc
    21361 ggccgctttg tcttcgaccg gagtgacttc cgcgctgtcc gccaaaagtc ggtgggcttc
    21421 ttcattgtca tcatccaagg acttggccac ggaggtcacg agtaccttgg ttttcgacgc
    21481 aagatccagc gcctcgtcca cgctgccgtt ggccgcctca gcctcgacct tggcacgttc
    21541 gcgcaggcgc ttggccagat cggccgaata ctgcgaagcg tcacgttcgt ccaaatgctt
    21601 gaccacgcca tcggacagtt gcgaatcgac gtgatacatc gaccccttgc cgagattcac
    21661 ggagaaccag tcggcgaaat gttccagcag ccagatacgt tcggtgttca cgccggtact
    21721 cggcaaatcc acgacttcgg tgatgcggtt acgaccatcc gtgcaggtgg cctcgatatc
    21781 cgtcaggttc acgtcggcgc gtacgtgcac gcccagcggg taggcgatgc ggtgcatcat
    21841 ttcacgcacg cggaaggagc cggtgccggc tcccaggtcc aacatgccga ctcgtacaat
    21901 cacgctggct ttggcggcga taccggcgtc caccaccggt ttttcccaat cgcgtgcaat
    21961 atcttccatg tccagcggaa tggagtgggt gtggaattta cgtacagccg ttgcgccttt
    22021 cgcttcgggc gcactttttt tcttctgttc gctcacatcc ctcattacta ctccctgcct
    22081 cccccgccgg caatcgtaag gcgctcaaat cttccgcgaa tcggacagtg tctcatatgc
    22141 tgtccgatat tcggtctcta taggtggaca acttggcgca ctatatggac tccgtaacgc
    22201 aaatagcgat ttcgcgggga tacactattg cccacacaac aaccacgttg aatatgggct
    22261 gtgggctcgc aacgactggc ctcaacaact gcggaggagc cgcagacgac cggtagaccg
    22321 agcaacctgt tattcggcaa aggaggttat atggcaacac ctgaagaaca gatcactccc
    22381 gctgacgccg ccatcgtcac taccgggacc ggccgaaagg gcccggagga gcatgatctt
    22441 cccgaaagcc tgaagtacga catggatctg tgtcttgaga tcctgcgtaa cgtactgggc
    22501 gaatacaacc ccgaactgct gtctacgttc gacaccgtgc gtcattacgc ggtggaggcc
    22561 agcgccgagc atttcgccga gctgaaggat ccgaaccccg accaggatgg cctgaaggag
    22621 gccgtgaacg tcatcgacaa catgacgttg cacgacgccc agctgctggc ccgcgcattc
    22681 gccacgtatt tccatctggc aaacctgtcc gaggagaact accgcgtttc cgtgctccac
    22741 gagcgcgaga acaacgtgcc cgttgaccag gcggtcgatc cgatcaacga gctcaccgtc
    22801 gcctaccacc agctcatcaa tgagactggc ccggccaagg ccaaggaact gctcaaccag
    22861 ctcgaattcc accccgtctt cacggcgcac cccactgagg cccgtcgtaa ggccgtggaa
    22921 ggcaagatcc gccgcgtttc cgaattgctt gaggagtaca agcgcctcgg cggctcggat
    22981 aagaaggaat gcctgcgccg tctctacaac gagatcgacg ccctgttccg cacctctccg
    23041 atcgcgctga agaagccgac cccggttgag gaagcggaca cgattctcga catcttcgac
    23101 aacacgctgt tcaacaccat ccccaaggtc taccgtcgct tcgacgactg ggtcctgggc
    23161 gataaggccg gtctcgtcga gcccgcttgc cccgcgttct tccacccggg cagctggatc
    23221 ggctccgacc gcgacggtaa cccgaacgtc accgccaagg tgagccgcgc cgtggcccgc
    23281 aagttctccg atcacgtaat cgccgccctt gaggaagcca cccgcaccgt gggccgcaac
    23341 ctcacgatgg aagccgagac cacgccgccg agcgccgagc tcaagagcct ctggagccac
    23401 cagaaggaaa tgagcgagcg tctgaccgac aaggccgccc tgatctccac caaggagatg
    23461 caccgcgcgg tcatgctggt gatggccgat cgtctgcact acaccatcga gcgcgatgcc
    23521 gacctcatgt accactcctg cgacgacttc ctcgccgacc tcaaggtggt ccagcgttcg
    23581 ctggccgccg ccggcgccaa gcgttccgct tacggcccgc tgcaggatct gatctggcag
    23641 accgagacct tcggcttcca catggtggag atggagttcc gtcagcactc cgt
//
LOCUS       DAWBMW010000009        23379 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_601_length_23379_cov_3.294246, whole genome shotgun sequence.
ACCESSION   DAWBMW010000009 DAWBMW010000000
VERSION     DAWBMW010000009.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 23379)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 23379)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..23379
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_601_length_23379_cov_3.294246"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    9436..9445
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    18038..18047
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4700 a         7237 c         6772 g         4650 t
ORIGIN      
        1 ctattatctc ggtgatcgct tggttcatcg aaaaagccaa tgcgcagccc ggcgcggaag
       61 gcggcgaagt gctgccgttc gacgccatcg tgatcatctt gatcctgatt gtcaatgctg
      121 tgctcggcta tatgcaagaa gccaaggccg aggcggccgt cgaagcgctc gcacaaatga
      181 cggccccgca gacctcggtg ctgcgcgatg gcaaggtcat gcgcatcaac accgccgtcg
      241 tggtccccgg cgacatcatc gtccttgcgg aaggcgattc ggtctccgct gatggccgac
      301 tggtcaacgc ggccagcctg cgcatcgccg aggcttcact gaccggcgaa tccgtgccgg
      361 ttggcaagaa gccggacaca ctcaccgaag ccaaggcgct gggcgaccgc gccaacatga
      421 tcttcaacgg cacttccgtg acgcaaggca ccggccgcgc aatcgtgacc ggcaccggta
      481 tgaatacgca ggtcggcaag atcgccgaca tgctctccgc caccgaagat gagaaaactc
      541 cgctgcagaa ggagatggat tacgtctcca agatcctcgg cattgcggtg tgcgtcatcg
      601 ctgtcgtggt gctggtggcg ctcgccgtgc tggaaggctt caacgacgtc catgacgtga
      661 ttgattcctt gctgttggct gtctcgctgg ctgtggccgc cgtgccggaa ggcttggccg
      721 ccatcctgac cgtggtgctg gcgcttggtg tgcagcgtat ggccgcgcac aacgccatcg
      781 tcaagaaact gcattcggtg gagaccttgg gctccgcttc cgtgatctgc tcggataaga
      841 ccggtacgct gacccgcaac gagatgaccg tggaacgtgt ggtcaccccg tccggcgaag
      901 tgcagatcac tggcaccggt tacgcgccgg aaggccgcat ggttatgacc ggcggcgtca
      961 gtcctgaatc cgagcaggcc cagattgtgg ccgatgaagt cgtcgccacg ctcgtggccg
     1021 gcacgctcgc gaacgacggc gaactacgcg aggagaacgg ccgttgggag attgtgggcg
     1081 accccaccga agtctcgctg atcgtggccg cacgcaaggt caaggccgat cgcaagatca
     1141 agcgatacac gcgcgttggc gagattccgt tcacctccga acgcaagcgc atgtccatca
     1201 tcgccaagga cagcaccgat tcagacaagc tcaccgtgtt cgccaagggc gcgcccgacg
     1261 tgctgttgag ctactgcacg cgcatccgcg tgggtggtca ggtccgtaag ctcaccgaag
     1321 gcgatcgcca gtcgattctc gccaccgtcg aacgtctctc ctccgaggcc taccgtacgc
     1381 ttggcgaggc ctgccgtccg ctggaaaccg gttctttggc tgatgtgccc ggtgtaagcg
     1441 tcaacgctgc aggccaggtg tccgacatcg ccgatcaggc cgaagccatt gaaaccgatc
     1501 tcatctggaa tggcatggtc ggcattatcg atccgccgcg tactgaggtg cgcgactccg
     1561 tgaccgaggc ccatcgtgcc ggcatccgca cggtgatgat caccggcgac cacccgctga
     1621 ccgccgcccg catcgcctcc gaccttggca tcatcgccaa ggatggcaag gccctgaccg
     1681 gcgaccagct cgaccagctg cccgatgagg ccgcgctcga caaggccacg tccgaagtgt
     1741 ccgtgtacgc gcgcgtggcc cccgagcaca agctcaagat cgtcgaatcc ttgcagcgcc
     1801 aaggcaacat cgtggccatg accggcgacg gtgtgaacga tgccccggca gtcaagtccg
     1861 cggacatcgg cgtggccatg ggcatcaccg gcaccgaagt gaccaagcag agcgccaaga
     1921 tgattctggc ggacgacaac ttctccacca tcgtggccgc cgtacgcgag ggtcgcgtga
     1981 tcttcgacaa catccgcaag ttcctgcgct acctgctgag ttccaatgtg ggtgaggtgt
     2041 tcaccgtgtt cctcggtgtg gtgtttgccg gcttcctcgg catccgccag ccggaaaccg
     2101 tgggcgtcac cgtgccgctg ctcgccacac agttgctgtg gatcaacctg ctgaccgatg
     2161 ccgcgccggc gttggctatg ggcgtggacc cgcagaccga cgatgtgatg ggtcgtaaac
     2221 cgcgtaaggt gacggaccgc gtgattgacg cctccatgtg gggcgacatc atctacatcg
     2281 gcgtcatcat ggccatcgtc acgctgattg gtatggacat gcacctgtcc ggcggcttgt
     2341 tcaccgaccg ctcggtggat gccatcggcc atgaagcgca gatgaccgag gcccgtacga
     2401 tgggcttcac gattctggtg tttgcccagc tattcaacgc gctgtcctcc cgttcgcatc
     2461 tgcagtccgc attcgtgggt ctgttctcca acaagtggct atggggtgcg attggtttgt
     2521 ccgtggcgct gcagctcgtg gtgatttacg tgccgttcct caacggtccg ttcggcaccg
     2581 tggcattgag cccgatggct tgggtggaat gcatctgcct ggccgcaatc gtgctgattg
     2641 cctccgaact gcgcaagatt gtgctacgcg cgatggccaa gcgttaagcc atcgcaatag
     2701 ccctgattgt gggcatatag cagtgggccg ggctctggta ttcagagccc ggcccactgc
     2761 tatatccaaa cggctcctca tggggcggta actatcccat ggggcggtga tttcctcatg
     2821 gggcggtcgc tgaaaacggc attattccgc gaaaaaggaa aatgtgctaa ccgccccatg
     2881 gggaagttac cgccccatgg gaaacaggag aacgctgcag acctcagttg ttgctgccgt
     2941 cggtgccgtc agtagaggtg gagttgtcgc cgccgttatt gttgtcgcta ttgttggact
     3001 cgtcttgaga cttctgctgt gcctcttggt cagccttctg ctgagcctct tcctgagctt
     3061 gcttggcggc atctgcctgc tgcttggcct gcatcgaggc ggtgacgtcg gactcggcct
     3121 tctgcagttt agaaatcgca ttattgacgt cggattcgga cggactggtc ccgttcatca
     3181 ccgtattggc ctcggtgatg gcgttttgca gcgcggtgcg ggtgttttca tcggccacac
     3241 tgcccgcgga attatccagc gtgccctgcg cctgcgaaat agccgagctc aggttcgatt
     3301 tcagtgtgtt ggtatcggcc gtcgcctttg aagtattaac ggccttgatc gcattgttga
     3361 tggcatccgt cttgtcagcc atgtcgctgg catcattctg cagcttttta gtgtgagact
     3421 tcaactcagt ggcgttgagg ctggaatcgc acttggcggt gtcagccgga ttgccggcgt
     3481 cttgcaacgc ctgattcagc gtggtgagcg tattggcatc ggccacatcg ctggtggatg
     3541 tttgcgcgct ggtttggcca ttggatatgg cggtggtgag cttcttctga gcattcgtgt
     3601 actgcttggc cgctaccttg cactcggcca atgccacggc ttggctcgcc ttgctgctgt
     3661 tggaatgcca ccagagcagg ccggcgacaa ccagcgctat cacggcgact actgctacgg
     3721 caatgctgat gatcttgcgg gtgttctttt tgccgctacc gccgtgacga ggctcggcgc
     3781 catcggaatc gtcctcatcg gagacctgat ccaacggaat ggagggaggc atcacagcag
     3841 gctgagcctg tgccgaagcg gccagggcgg ccggttggaa cacctgtgtg gctgcagcat
     3901 catcggcaga cgctacttgc gttgccggca tggtaacggt ctcaccggat acggtggatg
     3961 atgcgccgta tgcctgtgtg gccgcagctg aatcaggctg gaatgcctgt gtggccgcgg
     4021 tttcggcatt cggcatggcc tgagcttcga aaggctgtgt tgccatgacc tgatcaggct
     4081 ggaacgcttg tgttgcctcg gcatctaccg caggctgctc ggcctgattg ggttggaacg
     4141 cctgtgtcgc ggcgtttgaa gctaccgaaa caggcgatgt cgccggtgcc gctggtgcgg
     4201 ctggtgctgc tggcgtcact ggcgctgtcg gttccaccgg gcggaagctt tgcgtcgtct
     4261 gatcccaaac gtattgctgc tggccgggcg cagcggcgat tggtgtttcg gatactactg
     4321 gctgcgtggc tgccgccggc tgtggtgctt gttcggcaac cgcagccggg aattgcggcg
     4381 agaacggttg cgtcatggcc ggatcgatgg gctgcgtcgg ctgagcggct tgtacgggcg
     4441 gcacagcttg cgtgggttgt gaggcatacg ctacttgcgc gggctgcaca aactgtgtgg
     4501 gctgtgtggg ctgcgcagac tgcacagact gcgggaactg cagagccggt gtggcttgtg
     4561 ccgtgggttc tgtgctatat gctgccgtct cttgcccggg aatcggagga accgttggcc
     4621 tgaccggctt ttcgggctgc tggaagtcga actggaaccc gccatcgctg ttggtcatga
     4681 catctccttg ggattaagca aatggccggc atccgccggc cgaatggtac gtcgcaacgc
     4741 tcagtcgtcg atgtgcgcgc ggtagaaatt ctcgtaggaa cggctcggcg tcggaccccg
     4801 ctggccctga taatgcgagc cggtgccctt ggagccataa gggtgctcag cgggcgagct
     4861 cagctggaag aagcacatct ggccgatttt catgccaggc catagtttga ccggcaacgt
     4921 cgacacatta gacagctcaa gcgtaatatg cccctcgaaa ccggggtcaa tgaagcccgc
     4981 agtcgaatgg gtgagaatcc ccaatcggcc cagtgagctc ttgccttcca gacgggcggc
     5041 aatggacggg tccaacttga cgtattccca cgtggaaccc agtgcgaact cgccggggtg
     5101 caaaatccac ggttcgtcgg gagccacctc gaactgttcg gtgagcgcgc cttggttttc
     5161 cgccggatcg acataggtgt aggcgtggtt attgaacaaa cggaagtacc gatccaatcg
     5221 cacgtcgatg gaagcgggct gcaccatttc gggggtccac ggatcaagtg agatatgacc
     5281 tgcggactgt gcggcgagaa tgtcgcggtc ggacagcagc atgtgaaaaa ctccttgggc
     5341 aaataaatac tgccttcaag tctagcgcct tgggcggaaa cgtgagaggc tcctcacgct
     5401 tccgcggtcc atcaatcgca agtattctca ggtttttctc agagaaactt agaggtagac
     5461 ccagaaagca gtggttatat aaaagatgtc agcggaaagc tgatcaggag ccgcagcagt
     5521 tcccgacaat tgaaccccct ttcttcttct ctctcctttc tctccccggc gggcaaccgc
     5581 cggggttctt tttgtgccct gaatcattcc cccgaatgac cgccattccc aagccatagc
     5641 ggtacactag ctgtgtaatc ggcaggaagg ggggcaccga tggcccgcaa cgatgaggac
     5701 tacgatttct tcggcgatgg tcagtatgac gattcgcgca gctcacgtcg tgcacagaat
     5761 cgacgaccat ccaggcggga tgccggccgg cgattcgccg ctacaaaagc cagcagccca
     5821 acgtcgatat tgtgccgcag acttgccgtt gtcagcggac tgctagccgt cgtggtatcg
     5881 gccacctcgg cggttcatga gatcgcgcct tacgccattt cctttatgac ggcagtgcca
     5941 ggacgataca ttgcgttgat ggcggcggtg cttatcggca tcaccctgat tctggtcatc
     6001 gctgcgcgag tcaccatgcc tcggtacgcc actcacgggg gagtgggcgc gggcggcatc
     6061 atcgcactgg tgtgcgccat agtttgcttg gttatcggcg tggccgtggg cgtattgttc
     6121 ccggaaggac tgattcagca gtcgaacgac aaggcacccg tcgaagatac cgcacagatg
     6181 gagcaaggca tcgaacaagc tgccggcgcg tgcaaagaag gatggcaggg gcttgatacg
     6241 ggcggtttgc cgggcatcac caccgtacag atgtgcactg atccgagagt ggccttcgta
     6301 tcctttgaat cggaatcgtc cgcagccatc agccgggcac ctatcgaaag caagatttcc
     6361 gaactgctga gccaatacgc tgataatgag caaacgaaag gtgattggcg attgctgaac
     6421 ggcaaacgct ggatggtgtt cggcgaggcc agcaagatga ccgcactgca gcagcagtgg
     6481 ggcggcgaac ttgagacaat caccgcagcc gcagataccg cagacagcgg taacgcctga
     6541 cagattgccg gtatcccttg aaaagtccca aggcgattcg gcgcggatta cccaatgtga
     6601 ttgaaatcat ggtccatcgt tttccgcaca gcgcgcaaga cctctatatt ggaatggtat
     6661 gagcaaagaa gcgaatgcca gccagcctct gatcggccgc gaatccacca ctgtgcccgg
     6721 acgttttggc gagccactca ccgtggaaca cagcgtcacc tcgcgcggcg gcttcgacca
     6781 gcatgccacg catccgctgt tcctgtgcct gcacggatgg ggatccagcg aagaagacat
     6841 ggcggacatc atgcgcctca tcgctccata taacgacttc gtggcactgc gcgggccgct
     6901 gacactcgcg ccggcccgtg aaggcagtcc cgaccccggc aactacgcgt ggtttcacga
     6961 tgccctgcct atcggcgatg atcgtgacta cgacgcgtat gccgccgcca cagcagtcga
     7021 ccgatgggtg gccgacaaca ttcccgccga ccgtgacgtg gtgccgcttg ggttttcgca
     7081 aggtgggctg gtcgccgtgc atttgttgcg tatcaatccc gagcggtacc gggccgtcgt
     7141 ctccctgtcc ggcttcaacg cacccggtca ggtgcccggc acggcccccg ccgactctcg
     7201 cctggccgat tacgatatcc ccgtgttcta tacgtacggc aagaacgatg gcgtaatccc
     7261 aaaatacgaa ctgttcgcca ccgccgcttg gctggaagag catacgtggc tgaaaaccaa
     7321 gagctaccac ggtctggatc acaatgtgag tcttgaggag ttcgccgatt tacgccagtg
     7381 gctacttgac aacgacatta cttctggcgt gctgtagtca gccgccatta ctggaattgg
     7441 cgcggccgtc gcagcaatcg gacatcgagg gcgcttgtgg ccggcgcggt catagactgt
     7501 tctcccatga gcgaagtgag cactgcaagc actactgcac atactgaaga gaatcatacg
     7561 gaaatagcaa tccccaactt ggctcccctc agagaaggga gccagggaaa ctagatgccg
     7621 gaatcagcgt ccacttgcat aacgtagatg ttgcgcctca gggagcgggc ttgggctcgg
     7681 ttgatgtcgc cggcttcgta catgtcctga atcataccga gttcgatacc gtagctctcg
     7741 cgctttacgt cctcgacctg catgatggtt tccgtagtgc cgctcatatt cggacgtgcc
     7801 tgcaatgaag cctcggcacg acgataatcc agcaacagcg ccgaacaatg ctcggtgttg
     7861 tacgtatcct ggcccatctc gtcaataagc cggccgataa catgatgaat cgcctcgacc
     7921 tgcagttcgc gggtgcgcgc gaaaatctga tcctcgctca ccaatggtgt ggtatggcga
     7981 atcttggaca ccgtgcgttt ggcgtaattg ctcatctgcc gctggaacat gcgtaagcga
     8041 ccgcgaatct gcgataccgc atgccccgaa gtcggatcag tggaagtgtg acgcagcgta
     8101 ttcataatct ggtcaagcat gcgctcgcag gcttcgacgt tcagctcttg cgtggccgta
     8161 tccgccgccg gatgcgcctt ggtgtcggca agtcggtcgc gcacgaattc cttttcccaa
     8221 tgcagcgctt cgatctgcaa ttgctcgaat ccctgaggat cggtatcgcc gatgcgctgc
     8281 tgcaatcgcc cgatgcgctt ggtgtactgg tcgattacca tcagaatcgc acgacgattc
     8341 tctggcgtga tgcgtccggt caattcctcc accgtggcgc gtaacacatc gatggtcacc
     8401 ggaattattt ccgcgctggg atccttgcca cggttcggag ccaatagcgg taacaggaag
     8461 ttggccagca gcaaggtcac gataatcacg cctgacgcaa tgaaaatcag ctcgtcacgc
     8521 atcgggaacg gcgcaccgcc ggcaatgtaa tacgggatgg tgaacatcaa tgacagcgtg
     8581 atggttccct tggggccgcc aaaggtcatc actgcggccg agcgccagcg ttcgggtgtc
     8641 attttgcggc gctggccgga gattgtatct cgcgcaacgc gcagcatggc cgcaatccag
     8701 aagaatcgaa gcgcgattac caccagagtg accagcagaa taatgccgat cagcgcgata
     8761 ttgctgatat acgggtcaga ccagctggcc atcatggcgc gcggcagctg catgcccaga
     8821 agaatgaaca ccgcaccgtt gagcgagaaa ctcagcacac cccatactga agtggaaacg
     8881 atattcgtgc gtgccacatt cggccccacg ccggtgcggt cgaatcggat gaacaggcca
     8941 gcggccacca ccgacagaat gccggacaca ttgaattcct ccgcgcccaa atacagcagg
     9001 aagggcagga acagttccat cagaatacgt gtggtagtgg tttcccagcc gagggatcgc
     9061 acggtctcga atagccagtt ggccgctgct gctatcacca ggccaaataa ggtgccgcca
     9121 aagaaggaga tcacgaattc gccggcggcc tgccccacgg cgaattctcc agtgaaggcg
     9181 gcggccagag cgaactggaa gccgacgatg ccggaagcgt cattgaagag tgattcgcct
     9241 ttcagcacgc tgcgctgacg ctgcgtcagc gaggcttctt taccgaggga gctgacggcc
     9301 accgcatcgg tcgggccgag tgccgcgccg agtgctaagg ctgaggctaa ggtgatggac
     9361 ggccagacgg catgcaaagt gaatccgacc gccgccatcg tgacgatagc cagtccgata
     9421 gccagcgata ggctcnnnnn nnnnnggctc agatccagcg ttttcagcaa cgccgactta
     9481 tcgatttcgt gggcttccaa atacagcaat ggcgcgataa acagcaccat gaacagttcc
     9541 gggtcgagcg ttgcatccgg gaaaaacggc agatacgtaa ccagcacgcc cagcacaatc
     9601 tggaccagcg gcgtggagac tttgggcacg aagcgactga caaacgagct caccaccacc
     9661 gccgcaatga tgcataggat gagctcaaat accgccatgt tgccgccttc tcccttaaat
     9721 ttttctccac caaaagtata tgggaaccat gaagaaaacg ttaagcttgc ggccgattat
     9781 gcacatcatg agacgatatg agaaaaggca tacactcgtt ggtatggaat atgacgaggc
     9841 gatgcgccgt gcactggaac tggccggtca ggctgcagcc gctggtgacg taccggttgg
     9901 tgcggtggtg ctggatgcgg ccggccagat tgtcgggcgc ggatacaaca cgcgtgaggc
     9961 tgatggtgac ccgcttgccc acgccgaaat catcgccatg cgtcaggcgg cccaagcctt
    10021 gggcgcctgg aatctcgccg attgcacgct ggccgtcaca cttgagccgt gcccgatgtg
    10081 cgcgggtgcc tgcatccaga cccatatcgg caccatcgcg ttcggtgcct gggatcccaa
    10141 gctcggtgca tgcggttcga tttgggatat tccgcgcgat ccgcatatcg gccatagtcc
    10201 tgaagtgcac ggcggcgtgt tggaaggcga gtgtcagtcc atcctgactg atttcttcgc
    10261 ccagcggcga tagacacgga cgcattactt acctcacgca accaacacgc ggcaccaagg
    10321 caacatattc catttcttct accatcgaaa ccagtagtgc aatgccaatc cacacacgtc
    10381 gaaggagaca atatggccga gaacacctcg actttcaccg gtgccgccgc tgacggtacc
    10441 gcactcaccg ccgtctacct gacccagccc gccgccaatg tggcgatcgg tctggtgttt
    10501 gccggcagcg acctgccgca catcgtccac tggggccgcc cgctggccaa gcccgacacc
    10561 ctgctggccg cctatgacgc cctgaagccg cagcgcgtgt ccggcgcgct cgacgacacc
    10621 gcttggccgt ccatcctgcc cacccaggtc gaaagctgga tcggcgagca acgcgtggtg
    10681 ctgcgccgcg ccggcgtcga gctcttcccc aagttcaccg tgaccaacat cgagaccggc
    10741 ggcgtgctgg aagccaccct cgatgccgtg tccggcgaaa gctacaccga tgtggccggc
    10801 catgcccgcg ccaccggccc ggcccgcgtg cccggcgtca tagtcgccgc gcgcgatgag
    10861 gagcagggcg tggaagtcga atggcacctt gaactgctcc ccggcggcct ggtccgtcag
    10921 aaggccaccg taaccaacct gttcggtgca gacgccggag ccccgctcga gatcggcaag
    10981 atcgagctcg gcttcccgct gcccgaatcc gccggcgaaa tcctcaccac caccggtcac
    11041 catctacgtg aacgcagtcc gcagcgccag ccgctgaccg tcggccgttt cgaaaagccg
    11101 cagctcgccg gccgccctga cttcgacgca tccctgctcc tgaccgccgg cgtgcccggt
    11161 ttcggcttcg agcatggcga cgcctattcg gtgcacgtgg gctggagcgg caactccgta
    11221 ctgtccgccg aacgtctgcc ctacacgacc ggcgtaatcg gcggtggcga actcctgttc
    11281 ggcggcgaag tgacgctcgc cggccccggc gaaggccaga actcctacga caccccgtgg
    11341 ctgttcggct cctatggcga tggcctcaat gagatcgccg cccgcttcca ctcctacgtg
    11401 cgctccctgc atccgcgcct gttctcgcac ggccgtccgg tgatcctcaa cacgtgggaa
    11461 gccgtgtact tcgaccacaa cttcgatacg ctgaaggcac tcgccgacag ggccgccgac
    11521 tccggcgtgg aacgattcgt ggtggatgac ggttggttcg gttcccgccg cgacgacacc
    11581 tccggcctcg gtgactggca gattgcgcag gacgtctggc ccgatggtcc gaagtcactg
    11641 aaggcgctcg ccgactacgt gcacgccaag ggtatggaat tcggcttgtg gttcgagccg
    11701 gaaatggtca accccgattc cgatgtggcc cgtaaccacc ctgattggat tctctcgccc
    11761 accgctggcc gactgccgct gcagggccgc actcagcagg tgctcgatct gaccaatccg
    11821 gatgcgttcg actacatcta cggctgcatg gaccagcttg tcggcgagct tggtatcgac
    11881 tacatcaagt gggaccacaa caagctcgtc accgagcccg gttcccgccg ctccggccgc
    11941 ccggccgtgc acgcgcagac ccttgccgtc tacaacatct tcaagggcct caagaccgcg
    12001 catccgggcc ttgagatcga aagctgctcc tctggtggcg ggcgcgtgga tctcggcatc
    12061 ctagagcacg ccgatcgtat ctgggtctcc gactgcgtgg acccggttga gcgcgcggac
    12121 atccagcgct acacctcgct gctggtgccg ccggcgatga tgggcgagca cgtgggcgcc
    12181 agccccgcac actccaccca gcgcgccacc agccaggaac tgcgcatggc catggcgttc
    12241 ttcggccaca tgggcatcga atggaacctg ttgaaggagc cggacgaggc gctcgccaag
    12301 ctggccgtgt gggtggccga attcaagaag caccgcgact ggttcgccat cgacacctgt
    12361 gtgcacgccg actccaacga tccggccgtg cgtctggacg gtatggtgat gccgaaccgt
    12421 gacgccgcca tctaccgctt cacccagctg accaccagcc agacctatcc ggccgcgccc
    12481 gtgcatctgc ccggacttga ccccgagcgc acctaccgtg tcagcccgct tgacccgagc
    12541 ttggatttga ccggtctgac caacggccag agcactctcg gctggtggaa cgaggaaggc
    12601 gtagtcctga ccggcgaagc cctgcagcgc tacggcattc gtcccccgtc cctccacccc
    12661 cagcaggcag tattgctcaa ggctgtggcg ttaagctgac agcctagtag gctgtcagta
    12721 gccacggcct gagcgaagcg acagctgagc gaaggtggga ttgagccttg ccgataggca
    12781 agtccgtaat tgctggtcgt ggcggagtaa ctgtggcgaa gcaacgttct tggctcccct
    12841 ctctgagggg agccaagaac ggcctaccca atatgcttca caatcacgcg actggcggcg
    12901 gctttggccc agagctgcca gtcgtggttc ataatcgtga acttgaccgg ctgggtctgg
    12961 ctgtgacggt agaaacgaat gccgtcacgt agcgaatcgg tgccgagctt ggccaaaaac
    13021 gcggactccc cggcaatcag cacatgcccg ggcatcgcga tgttcgccac cgtagccacc
    13081 agcgatccca accggaaaca cgtccggttc accaactgcg tggcctgcgg cacgcgtccc
    13141 ttggcatcgc gcgcaaagtc ctcaaaactg accgcgcgac ccacaatcgc agagtactgt
    13201 tcggcgatcg aatcgtcagt caagcattgt gaacagccga tatgtccgct ggtgcagcgc
    13261 ggcccctccg gatcgataag cacgtggccc accagaccat agctcttatc cgggtactgt
    13321 actggcttgc cgttcacggc cagcgaataa ccaacaccca caccgatggt caccacggcg
    13381 aacatatcca cgccgacacc cggcccgaac caggaggcat ccaccagcag agaatcgata
    13441 tcattgaaca ccccgcacgg caggccggtc gcctctgtgg ccatcgtccc caagtccgtg
    13501 gccccgtccc aatgcaggaa cggggcgaac gtcacaatcg aatcgtcgac cacatgccca
    13561 cctaccgcaa cgcccaccgc agtgggggag ggtagtccgg aagaggcgat atcggcggaa
    13621 cattcgcgca ccagccgcgt gatggccgac accacatact cagccgattg atccgccccg
    13681 atcggcacct cgtgccggcc ggacaccacc tcgccatgcg cgttcaccgc agcggccaca
    13741 atcgacatcc catgaacctt gacaccaatg aaggtgcgtg cgttcgaaca caacaccagc
    13801 gaagtctgcg ggcgtccgcg acgatccgaa ctttcccttg gcgtgaatcg ctcgggcagc
    13861 ttgcctgacg ggccggcctc ggccggaagc tcctcgatca ccccggcgta gatcagatcg
    13921 ctggtgatgc gggacaaggc cccttggctc agtcccagca tctgtgccaa cgtagtgcgg
    13981 gctatcggcc cgtattgggc aattgccgcc gccacgcgat gcgtatgctc ggatccggaa
    14041 aaccactgtg gaatcgcgtt ggcatcagac atgcaagccc ccctcgctgt atttattgcg
    14101 ttaatcacaa aataccgcgc tttttgttgc ttgcgcaagt gggctcgctg gcgctgggaa
    14161 tgaaatggcc gaactgttac cttggaaagt ggaagaaccg ccggcaccga agccggagga
    14221 aaagaaagat aaaggagtca acaattatgc tgtccgattc cccacatgca acgcctagac
    14281 tgaagcgtct ggccgtgcgc accgccgccg tggtgtgcgc gatcgccgtc ggcttcggcc
    14341 tggcaggctg cggctccagc accgcaggca ccgtcacgct ggatttcttc cagttcaagt
    14401 ccgaggccgc cgaccagttc aagtccatgg tcgccgactt tgagaaacag aacccgacca
    14461 tcaaggtcaa catcaacaac tccgccaacg cccagaccga tctgcgcacc cgtttcgtca
    14521 agaatcgtgt gccggacgtg atcaccttca acggcgacat cagcttcggc aacttcgccg
    14581 cctccggtgt gttctacgac ttcaccgacg aacccatcgt ggacaccctc aaccccggca
    14641 tggtgcagat cgccaagaat ctggtgcaga ccaccgattc ctccaagaag cgcctgtacg
    14701 gcctgccctt cgcgggtaac gcctccggct acatctacaa caaggctctg ttccgcaagg
    14761 ttggccttga cccggagaac ccgccgacca cctggagcga gttcaccgat atgctcaaca
    14821 ccttcaagga cgccggcatc aacccgctgc agggttccgt ggccgacgcc tggaccaccc
    14881 aggcaccgct cgcctccctg gccggcacgc tggttcccga atcgaagtac accgagctca
    14941 agcaaggcaa gaccacgttc caggaactgt ggaagacctc agtcgagaag gaatccgagc
    15001 tcttcaccta ctccactgcg gacaccggcg tcacctacca gcagggcacg cagaacttcg
    15061 cccaaggcaa ggccgcgatc atcccgctcg gcacctacgc catcccgcag atcctgctga
    15121 tcaaccccga tatcgaactt ggtttcgccc agatgccggc caccgatgac gccagcgagc
    15181 agatcctcac cgccggcgac gacgtgatgc tcaccatcgg tgcgaacacc aagcacccca
    15241 aggaagcgat gaagctcgtc gaattcctca tgcagaagga tcagcttgac gcctacgccg
    15301 acgcccagtc cgccatcacg ccgctgaagg acacctactt cggcaacgat gcgctggaaa
    15361 ccgtgcgccc gttcttcgag gaaaaccgac tggccgactt ctgcgaccac tacattcctt
    15421 cgtccatcaa catcggcggc tacctgcaga ccatggtgac ctccggcaac accgaccgtt
    15481 tcctgaacca gatgcagacc gaatgggaca aggtccaagc caggaccttc gagtgatcgg
    15541 taggagagga gaacatcatg tcacacgcaa ccgcaaccaa gaccgctgcc aagaaacccg
    15601 ccaagaagaa ggtctccgcc ttctccaccc gcaaagtgga ccccgcctac tactggatgg
    15661 tggttccggc ggccatcatc ttcgccttct tcctgtacct gccatttctt gatggcgtaa
    15721 agtattcctt caccaactcg cagggctacg gcgattacaa gttcatcgga ctgaagaact
    15781 acatcgccct cttccaggac aaccgagtgg gccatgccta cctgttcacg ttcctcatcg
    15841 ccattctcat cacggtgctg attaatgtga tcgccctgtt cctgtcggtg ctgctgaact
    15901 cgaagatcgc cttcaagaat ggcttccgag ccatcttctt catcccctac accctctccg
    15961 tgctggtcat cggctacgtg ttcaagtaca tcttcatgaa ccccctgccc gagctcggca
    16021 aggcgctggg catcaagtgg ctgtccacct cgctgttgac caatgagcaa ctcagctgga
    16081 tcccgatcgt cttcctcgcc gtctggcagg gcatcgccta ctcggtgctc atctacctcg
    16141 ccggcctgca gaccatcgac gatgaaatct acgaggccgc cgccatcgac ggcgtcaatg
    16201 cctggcagaa gttctggaag atcaccttcc cgctgatcgg accgttcttc accatcaacc
    16261 tcgtgctgtc catgaagaac gcgctcggca ccttcgacca ggtcgtcgcc ttgaccgagg
    16321 gcggcccgaa ctcctccacc gaaaccgtca cctacctcat ctggaagggt ggcctgaccg
    16381 gtggcgagta cgcctaccag accgcaaacg ccgtgctctt cttcatcgtg ctggcgatca
    16441 tcgccttcgt gcagctgcgc atctcccgca gccaggaaca gatctgattt aggggaggat
    16501 aagaaatgac tactgcaaca gttaccccgt ccaagtccgg caagcccgcc aagttccgca
    16561 gggaccacaa gatcaactgg tggctgaccg ccgccgtggc cgtgctgagc ctgaccattc
    16621 tgatcccgct ctacttcacc atcgtcaccg cgctgaagac cccggccgaa gccggcacct
    16681 tcgccctgcc cacctcgtgg cagtggcata acttcgccga cgcctccgcc aaggtcaact
    16741 acccgaaggc cgcgctcaac tccgcgatca tcacggtggc cgccgtggtg ctcaccctgc
    16801 tgaccaacac cttcgtggcc tacgccgtgg cccgcaacat ggacaagcgc ttcttccgct
    16861 tcctgtacta cttcttcatc gccgccatgt tcgtgccgtt cccggtcgtc atgctgccga
    16921 ttgcgaagca gatgggctcc ctgcacctgg acaaccaggt cggcctgatc attctgtaca
    16981 cggtgcttgg tttgggcacg aacctgttca tcgccaccgg cttcatccgt tcgattccgg
    17041 tttcgctgga agaagctgcc cgcatcgatg gcgcatccac ctggcgcatc ttctggacca
    17101 tcatcttccc gctgatgagc ccgattaacg ccaccatcgc catcctgacc gcgctgtggg
    17161 cctggaacga cttccttcta ccgttgatca ttctgaccga tcagtcgaac cagaccatcc
    17221 cgctcgccca gtacgtcttc agctcgcagt tcgccaccaa ctacccgatg gccttctcca
    17281 gctacctgat ggccatggcc ccgatcctca tcgtctacat cttcgcccaa aagtgggtcg
    17341 tcggtggtgt gatgagggga gcggtgaagt aaaacagcgc aggcgctgct ttacttcacc
    17401 gcgtcgagcg agacgagccg attaaggaaa cagccccgtg ggctgtttcc cggcccgggc
    17461 ggccggatag gtcgcgtact cgcgaggtca tgttgggtct ggccgtaggc cagtcctgaa
    17521 ttgcaggact ctgtgaagta aaggtccgct tccacccaag cctcctccgg ctctcaccgg
    17581 aggaggctct tctgtcctgt tgccatactg gaaaccgcca caataaaggc gacggtcggt
    17641 tgatgtggcg gtggcgcaca aaggtcatgt ggttttgctc gttgtgcgaa tgaaaaagtg
    17701 acgaaaccgt acggcttacg gtgcaattga gcgcatctgc tgtgtcgccg tgccactggc
    17761 ttgtcccggt ccacatatca accgtttgac gcgcgaaccg ggacagaatc cgctgtccag
    17821 gttcgcggtt tcgggcgatt ttcacgaacc gggacaattt tcattgtccc ggtatgaatg
    17881 tcaatcgttt tatgtgaaaa ccgggacaac atgttgattc cgggatcggc tctattccgc
    17941 cgattttgta ttacggagca tatgacctgc ggagattggc ttgtccctgt ttgtaaaaaa
    18001 ggacaaaaat cgcgaaccgg gacagcggat tctgtccnnn nnnnnnnatt ctgtcccggt
    18061 tcagacggca atcgattgac atacgaaccg ggccaacgcc gaagtccgga gcggttcccg
    18121 aggcggggtt tgcttgaaat ccggtgtgcc agcgaaatcc ccaccagatt cccgcaggcg
    18181 tgtcaactaa atcatctatc cccgcaccag tgggttcaag cagacaatcc aagcgcgccc
    18241 gaagttgctc ttgcgcgtcg caatcagaag aaccgttaat atgtgagctg ccgcaggtca
    18301 ggtagattat ggtggcgaag tggcggcgga acaagcgggc gagctccttc ataggttcga
    18361 gcctcgagtg catcatccac gagcacaggc gatatagcca cacccacgcc tcgaacagga
    18421 gcatggaccc tctgccgggc tcgaagccga tcgtgatgtg cggctcccgc ccgtcgccgt
    18481 cggcgtcacg gaagtccgca gacccgaccc tccaaggcga ccgcagccga agcgcggcgg
    18541 tgaacaggtg agaggcatcc atgcctccag cctacccgac caacatcacc ccacggcaca
    18601 gtcacccacc acaaacagcg aaagggccca tatgttgcta aatagcatgt gcagcttatg
    18661 agaattgagc cgtagcagtg ccttccggct ctttgcagtt tttgtttccg gcacaccaac
    18721 gcagttcccc aatggaaatg taattcagcg ctttttagac tatgagcaac aggcaatgta
    18781 gaccataaat aatggaggag ccaacgatga ccacttcaca acgcacagta attccagatt
    18841 ccatccgcac caacggtgcc accccaaatc cgtggtggtc caatgcggtc gtttaccaga
    18901 tttacccgcg ttccttccaa gacaccaacg gcgacggcct cggcgatctc aagggcatta
    18961 cgtcacggct cgactatctg gccgacctcg gcgtggatgt gctgtggcta tccccggtat
    19021 acaagtctcc gcaagacgat aatggctacg atatttccga ctatcaggac attgatcctc
    19081 tgttcggcac actcgacgac atggatgagc tgctggccga ggcccacaag cgcgggctca
    19141 agatcgtgat ggatcttgtt gtcaatcaca cttccgatga acacgcctgg tttgaagcgt
    19201 ccaaaaacaa ggatgatgag cacgccgact ggtattggtg gcgtcccgcc cgtcccggca
    19261 ccactgccgg cgagcccggt gccgagccca accagtgggg ctcctacttt ggcggttctg
    19321 catgggagta ttgccctgag cgtggcgaat actacctgca tcagttctcg aagaagcagc
    19381 cggacctcaa ctgggaaaat ccggctgtgc gccgcgcggt atacgacatg atgaactggt
    19441 ggcttgaccg cggcgttgac ggcttccgca tggatgtgat tacgctgatt tccaagcgca
    19501 cggacctgtc tggcaagctg cccggcgagt acggttccga gctggacgat ctgcccgtgg
    19561 gtgaggaagg ctattccaac ccgaatccgt tctgcgcgga cggccctcgc caagacgaat
    19621 tcctggccga aatgcgccgt gaagtgttcg aaggccgtga gggcttcctc accgtgggcg
    19681 aggctcccgg cgttaccgca cagcgcaacg aatacatcac cgaccccgcc aacggcgagc
    19741 tggacatgct cttcctattc gagcacgttg attttgactg cgaaggcgtc aaatggaagc
    19801 cactgccact tgacctgccg aagctcaaga gcattatggc cggttatcaa gcggccgtga
    19861 agaatgcagg ctgggcaagc ctgtttactg gtaaccacga ccagcctcgc gtcgtttctc
    19921 gttggggtga tgactcctcg gaggaatctc gtgtgcgttc ggccaaggca cttggcctga
    19981 tgctgcacat gcatcgcggc accccgtaca tatatcaggg cgaagagcta ggcatgaccg
    20041 acgcccactt cacgcgccta gaccagtacc gtgacctcga gtccctgaac gcttaccgtc
    20101 aaagggttga ggaagccaaa gtgcagtcgc ctgaatcgat gttggcgggt atcgccgcgc
    20161 gcggtcgtga taattcgcgt accccgatgc aatgggacgg ctctgtctat gccggtttca
    20221 ctgcgccgga tgccgctaag gagccatgga tctccgtcaa tcccaaccat gcggcgatca
    20281 acgcggccgg cgaattcgac gatccggatt cggtatatgc cttctacaag cagctcatcg
    20341 cgctgcgcca cgacatgcct gtcgtggcgg ccggcgactg gcatctgctc gatgcggatg
    20401 acgcgcatgt ctacgccttc acccgcaccc tcggcgatga gaagctgctg gtcgtggtca
    20461 acatgtccgg ccgaaccgtt gatctaccgc gcgaaaccgc cgaactggcc gccgccggaa
    20521 tcaccgagtc gaacgtggta atcagcacgt atgatgcccc gcacgccgtg gcctccctcg
    20581 ccaaccgtga gctcgatcct tgggaggccg ccgtcattca gctgtagcga ttggagcgat
    20641 tgtagcggat tgagtacctt tctccgttca cagagatact tcagggctat tgcgaaatgc
    20701 tatccgcctt gaaagaggta ccgtaacgtc aaaaccccga gtataagcgt tgaaaaatca
    20761 aggcttatac tcggggtttt cgatgctcgg tacccctgtg aaaagcagat agctgtctgc
    20821 aagaggccga atgtacctct gtgaagcgag atagccggtg tctctcggca ttgtggtgct
    20881 tacaatggaa agctatggaa caaaaggatg ttttgaaggc gctgcagcgt gatcatgcag
    20941 ccgaactgaa gctggccgcc aaacgtctca aagggactgc ccgtcatacg gaaattatcc
    21001 cttcgccggt attgagcgag atgaccggtc atgagattct cctgaagccg gaaaacctgc
    21061 aggtcaccgg atccttcaag atccgcggtg cctataacaa gattgcttcg ctcaccgaag
    21121 agcagatcgc tcacggcatc gtcaccgcgt ctgccggcaa ccatgcccag ggtgtggcct
    21181 atgcggcccg cgagcgtgga gccaaggcca ccatctgcat gccgcagatc accccgccgc
    21241 tgaaggtcga tgccaccaag gcctacggtg ccgacgtggt gctgtacggc gatgtgttcg
    21301 atgaatccgc cgcccacgct gctgagctcg ccgacaagga aggcatgatt tacgtgccgc
    21361 cattcgacga ttacgaagtc atctgcggcc aaggcaccat cggtctggag atccttgaag
    21421 acgtgccgaa cgtcactgac gtggtcgtgc cgctcggtgg tggtggcctg ggtgccggcg
    21481 tggcgctcgc catcaagaca ttcaagcctg aagtgcgcgt aatcggtgcg attccggagg
    21541 gctcaccggc ctggaaggac tcactggccg cgggtcgtgt gtctccggct gaccatgtgg
    21601 ttacctccgc cgaaggtgtt gcggtcaagc gccccggcga cctgcccttc gctctgatta
    21661 acgagttcct ggacgatctg gtcaccgtca ccgagcgcga catcaacgaa atgattctgc
    21721 tcatgctcga gaagcataag ctggtggtcg aggcggccgg cgccgtttcg ctggctgcgc
    21781 tggaacacct gaacctgcgt tcgcgcaagt tcgccgccgc caccggcccg cacgtggtgg
    21841 ttccgattat gtccggcggc aatatcgaca ccgtgaccat gggtgcggtg attcagaaag
    21901 gtatgatcgc ccgcggccgc atcatgaact tcgaggtcga gctgccggat actccgggcc
    21961 agctcgtcaa ggtggccacc ctgcttgccg agcagcgcgc caacgtcatt cagcttgatc
    22021 acgaccagtt caaggcatcc ggccactaca ccaacgcggt gtctctcggc gtcacggtcg
    22081 agaccaacgg ccccgagcac atcgaccgca tcctcgcggc actcagggaa gccggattcc
    22141 agcccaagcg catctactga ttccaatacc gctggcggga agccgcgcag aagtgtgctc
    22201 caatacctcc cgctggcggg aggtggcgca aaagcgccgg tgggtggtta accgacaatc
    22261 atcagttgct taacccaccc accggcgctt tcgttatgct gagggctact tgttctccgc
    22321 aatcgtctcg tccaccaact tcggcagggc aacggcgata tcctcatgaa tcaagcggct
    22381 cgcaagatgg tcatattgcg tgtgacccat attcataatg gtgatgggca cgcccgcctg
    22441 cgcggccacc ggcacaatgc tcgccgccgg gtacacctca agggtcgatc cgattaccca
    22501 tagttcgtcg gctttggtgg ccagactgta cgacttctcc atcgcgccgt ccggcaacgc
    22561 ctcaccgaaa tacaccacat cggttttgat aatgccattg cacggcatat cgccacgata
    22621 cttcagtttg cgatggcaat gtgggtcggg ctcttcatcc aaacgggcca tgatgtccgc
    22681 cgtggcatat tcctgatggc acttcatgca atgcgaggtg ccgatagtgc cgtgcaggtt
    22741 cacgatgacg ttgtcgctgt tgccggcctt ctcatgcagc gcatcgaagt tctgcgtggc
    22801 cagcagcgtc agcatgccgg cttgttccag cttgaccagc gccttatgcg cagtgccggg
    22861 ctgcgcattc catactggcg attccttttg ccagcgccag gagtactcgc ggtcctcttt
    22921 gttgcgcagg aacagatcga tgtcgtaaac gctcatttgg tcaggatgct tggtccacac
    22981 accgtccgga ccgcggaaat cagggatgcc ggcggacgtg gaaatgcccg ccccggttaa
    23041 tacagcaatc ttcttggtca taactacagg tatacccgcg attcatgaac gaaatccgat
    23101 gacggagcgc tgtcatgcgc cgattgtgcg ccggcagtgt ggttgccgag agatgaacgc
    23161 agggccgaca agctgaaaac tctcgcgaca cgccagccga atagtggatt tgatgctttt
    23221 cagaaacatt tgaggggtta acctagcctc atcagagcaa agtgggctct gtgcgacacg
    23281 ccgagggaac cgcgctgtta tgccgattcc tggggttttt gtgcgccttg atttgcgtga
    23341 atccggacat gcgtgtaagt tattcacctg ttgccgctc
//
LOCUS       DAWBMW010000010        23031 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_607_length_23031_cov_3.327603, whole genome shotgun sequence.
ACCESSION   DAWBMW010000010 DAWBMW010000000
VERSION     DAWBMW010000010.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 23031)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 23031)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..23031
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_607_length_23031_cov_3.327603"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2227..2236
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10208..10217
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11240..11249
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11567..11576
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4698 a         6880 c         6896 g         4517 t
ORIGIN      
        1 acttacaccc ggctgggtaa tcatgcaatc ttcggcgtgt cgcatgggcg gcagggatat
       61 atcgcttgtg ggtctcctgc gggcgccgtg atctttgagg gtggcggcac tcccctcagt
      121 cacttcgtga cagctcgtcc gcaattaagg acgggccttg cctgcaacct gctggttcga
      181 ctatcgtctc accttcgact acaaacagcc cactgggctg tttgcttaac gtctcagcct
      241 cagcggggga gcagagagaa gttcggcaca agccgcaacg aaggagaaaa actcacaacc
      301 ctggccggcg ctcgccgttg ccgtagaagt gggccaacgc ttcgtcgcgg aactcatcaa
      361 agtatccgcc gtcgatcgag gcgcggatgt cgtccagcag cttcacgaag aaatgctcgt
      421 tgtgaatcgt ggccaacgtg aagccgttga attcgcgtgc gcgaagcagg tggtcgacgt
      481 atgcgcgcga gtagtgacgg cacgtgtagc agtcgcatcc ttcctccagc gggccgaaat
      541 cgaacttgtg ctgggcgcgt ttgatgttgt atcggcccga cctcgtgtag atcgcgccgt
      601 tgcgggcgca acgtgcgggg gccacgcagt cgaaggtgtc gccgccgttc tccacgcagg
      661 cgaagatatc gtccacggag gcgatgccca gcacatggcg cgggcggttc tccggcatcg
      721 cgtcgcagat ccacgcgcag gtgtctccaa tgatgcgctt ctcgattgcg ccgccgattc
      781 ccacgccgtc gaagtccagc gaggcgatct gcgaggccgc gcgccggcgt agatcctcgt
      841 aattcgcgcc ctgcaccacg ccatacaagg cctgataggg cttgcccagc cgctcagccg
      901 tcagccgctg gtgctcgtcc acgcatcggc gggcccagcg gaaggtgcgc tccaccgagt
      961 cctcctggta ggagcgggtg ttcatcagcg tggtgagttc gtcgaacgcg aacatgatgt
     1021 ctgcgccgat tttgtgctgg atgcccatcg aaatctcagc ggagaaacga tgtgcgtcac
     1081 cattcaaagg cgacttgaac gtcacgccgt cctcatccac ccaggccatg cgctccttgc
     1141 ccttggcgat gatgtcgtcc gacttcatgc cggtcacgtc cattgccagc gtcttcttga
     1201 agccgacgcc cagcgacatc acttggaaac cgccagaatc ggtgaacgtg ggcccatccc
     1261 agttcatgaa acgcgccaag ccgccggccg cgtccagcac gtcctcgccc gggcgctcga
     1321 acaggtggaa ggcgttggcc agcaggcact gtgcacctag atccttcatc tgctcgggca
     1381 acacggcctt catcgcggct tgcgtgccca ccggcacgaa ggcaggcgtg cgaatatcgc
     1441 cgtggggcgt gtgaatgatg ccggtgcggc cgtatcgggc accgtctcgc ccaagcccgc
     1501 tggccgtgga tggcaggcgg gtgatcgttt cgaaatggaa ggcgctgcgg tcgccgggct
     1561 gtccgggctg tgcgcccaac ggatgttcaa gaaggctgat cgtcatatgt tcactttagg
     1621 cagaagggcc agaaaagggc cggaaattgt gaagaactcg gtttcgaggg gcggatttcg
     1681 ccgctcccga acgtctactt ccgcttcaag gggctcccgg tcaagtaggc cggctgcaaa
     1741 aatggcggaa tggcgttaaa tgagggccct tgatactcgg aattgaccgc gcgaacagcc
     1801 ccttgaagtg caagtagatg ttcccgaaac aggaaatcag cccctcgaag ccgagatgcc
     1861 cataagactc tgcgatgatt acgcatagac tttcaatcaa cgttcagaaa acctccagat
     1921 tgactgcctt tactactgag gtagcacacg agaagtggca atgggcaagt gcccgaatag
     1981 ccgccgaaca gacgcgatag gaatttagga aaaggagcag caatggctga cgaatttaac
     2041 ccgcagggcg tcgaccagac cccgcagaat aacgaaaacc ccaataatac tgccgatacc
     2101 ggcaacatcg agccgaagaa cccatttgta ggcggcgcct cggttgatgc cggcaacgcc
     2161 agcgattcca gcgagcagca agccccggtt tcctcggccg ccctcgctgc cgatcagcag
     2221 cctacgnnnn nnnnnnaagc cgttaccgac accgccgcca ccgagcccat cccggactac
     2281 gcctccgcca agggcgagag tacggtggtc tcctcctcgc cggcttctcc ggcagcgccg
     2341 gccgcagacc agacctcggc cacaactccg ctgtaccgcc cggccccgga atacggcgct
     2401 tatggaccga ccccgactca ggcacagggc cagcagggcg gccaggccgg cagcaatgcc
     2461 cagcccacgc agcaattccc gttcggtcag cctgcacagc aaaccccaca gggccagcag
     2521 ggcaaccgca atccctacta caccaataac ccgcagccgc agggcaacgg caatccgttc
     2581 aatccgccca cgcagccgca gcagaacaac ggcaacccgt ttgccagcca gtccggtcag
     2641 aatggccaga atggccagca gccccagcag ggcggtctgt tcggtttcgg cgctcccggt
     2701 accggtacgc cgaacggtca aggtccgacc cagccgggac agcccggtca gcccggtcag
     2761 cccggccccg ccaagcaggg catgtccaag acggcgagca acatcctcat cgccgtggtc
     2821 gccgccgttc tggctgcggc cctctgcctg ggcctcggct acggtgccct caccagcggc
     2881 ctgatcacct tgccgaccag caactcgctt accaacgtca gctcgaataa gtccggctcc
     2941 gggtccgcca ccgccaagag cggcgaagcc cccgactggc agactgtcgc cagcgatgtc
     3001 tccggctccg tggtctccat ccagaccgcg ttgagcaacg gcaccgccaa gggctccggt
     3061 gccatcatcg acaccgaagg ccacatcatc accaacaacc acgtgatctc cggtgcccag
     3121 cagattcagg tgacgcttgc caacggcaac atgtattccg ccacgctcgt cggcactgac
     3181 gccaccaccg atcttgcggt catcaagctc gacaacccgc cgagcgacct caaggccgtg
     3241 gaattcgccg attccgacaa gctcgccgtc ggcgagaacg tgatggccat cggccaccgg
     3301 catcgtctcc gccctgaacc gcccggtgac cgtgaccgac gataacaaca acgagatcgt
     3361 caccaacgcc gtccagatcg acgcggccat caaccccggc aactccggcg gcccgacctt
     3421 caacgcggcc ggccaggtga tcggcatcaa ctcctcgatc gcctccaccg ccacctcctc
     3481 cgactccgcc ggctccatcg gcatcggctt cgccatcccg tccaacctcg tcaagcgtgt
     3541 ggccgacgaa atcatcaagg acggcaaggt caagcacgtt gccctcggcg tggtcatcaa
     3601 gtccgacacg gttgaagccg acggcgtgac ccgcggcggc gcgaccatca ccaagtccag
     3661 cgccaccggc agcgcggtcg tctccggcgg cccggccgac aaggccggcc ttaaggaggg
     3721 cgacaccatc gtggccttca acggcaacgc cgtgaacaac aactactcgc tgcttggtta
     3781 cgttcgtgcc gcggctttgg gagacaaggt gacgctgacc atcgtgcgcg acggcaagac
     3841 catgaatgtg gacgtgacgc tcgatcagga ggagtcgtcg gtgaacggtt cgtccggctc
     3901 cgattccaac ggcaacagcc agaataacca gaacaaccag aacggcaatg gccagaacgg
     3961 ttacggtaat ggcaatggca acagcgatgg cggcaccggt gacggcggcg gcttctccga
     4021 cccgttcggc ctgtggtaaa gccgacttga caacgtcaca cggtgttgag cggtaaccgc
     4081 tgaacaccgt gtcagacaag caaaggcccg tggcagtgag caaactcctg ccacgggctt
     4141 ttcgtatgcg ctattgtgcc ctgcgcccat gccgccaacg tagtttcgat atccggcatg
     4201 tcgcacaacg ctcgttgata gggtaggtca agcactgagt tcgggcggtg ctccgaacaa
     4261 agcgcataat tcaatacaaa actgaacatt tctttacgcc gtgtatcgga aggtcgcgcg
     4321 ggagccgagc aaagcgaagc cccaggcaaa gccgccggtg ggttaacaag gcgtgaccgc
     4381 agcgccgatt gcgcgggcca cgcttgccgt accgcaatcg actccgtata cgagcgtaag
     4441 gagcaatgat gggcgcactc atcagattct tcaccaaaat tctcgacatc tgcaagcttg
     4501 tgccgatgct tccggtcgtc gtcatcgccg caattccgct ggctttactg ggcgattggc
     4561 gtccgtgggg caatgcgatg gcgattccgg tacttggcaa tccgggcttc ggccagtggc
     4621 tggttatcgc attggtgctg attatcgtga ttgacactgt gcgcggcatg attgacgatt
     4681 tgcgtcatgg gcaggttgga gtcgatgtgc tggccgtggt cgcgatcctc tccaccgtgg
     4741 ccgtcgccga atactgggca agttgggcgg tgacgctgat gatcacgtcc ggcgaggcca
     4801 ttgaggaata cgctcaagcc aaggccgagc gcagcctgac cgcgctgatg gaggccgcgc
     4861 cgcaaaccgc gcacgtcgtc aacctgcctg gcgtggggcg tgggtttgct gccggtaagg
     4921 gcgattcgtc cgatggcttc cggcgtgtgg gcttggcatc ggctgccgcc gccgcgcacc
     4981 gcttcgacac ggtgccggtc gagcaggtgc agctgggcga cgtactcatg gtgttgccgg
     5041 gcgaaactgt gccggttgac ggcgagctgc tgtccggcac ggccacgctc gacctgagca
     5101 atatcaacgg tgagccggtg ccgcgtgaag tgttcgccgg cgcccgcgtg atgtccggcg
     5161 cggtgaacgg ctcgactgca ttgaccatgc gtgccaccca ggtggccgcc gattcgcagt
     5221 accagcgcat cctcgagctc gtggcctccg cgcaggagtc gcgcccggcc gtggtaaaaa
     5281 ccgccgaccg cttggctgtg ccctttaccg tgctctcgct gatgattgcc ggcatcgcat
     5341 gggcgatgtc cggggtgccg acgcgattcg cccaggtgct ggtgctggcc acgccctgcc
     5401 cgctgctcat cgccgcaccg gtggcgtaca tcgccggaac cggacgcctc gccgcagccg
     5461 gcgtgcttat caaagcgcag gacgtgctgg aaaaccttgg ccgcgtcacg caggtgttct
     5521 tcgacaagac cggtacgctc accgtcaagc agccgcaggt cgtgcgcgtc gagatgctgc
     5581 ccggtgccgc aacccgtctc aacgaagacc atgtgctgat gatggccggc gtggtggaaa
     5641 gctactcggt gcacatcctg tccaaaggca tcgccaaggc cggaacggag gcgatggccc
     5701 gattgcgcca gcggttcgag gatggtcagc ggctctgccc cgagccggag gccagctggc
     5761 ccggccatgg acgggagtat ccggtcatca agaatatcaa tgaagatgcc ggcaaaggcg
     5821 tttccggcga ggtgaacgga catgcggtgc gcgttggccg gctgtctttc gcggccgccg
     5881 gggaggaagg attcttggcg gtcgatcgga ccgcccccag tcggtctgag gatgacctgc
     5941 gcacacgatt tgggctgctt cagccggacg aaatggcgtc gtatgtgtct gttgatggtc
     6001 agctgatcgc gcgcatcgtg ctgcgcgatg tgccgcgcgc caacgccaag gcagcgctcg
     6061 ccaaactgca tgagttgggc gtcaccaagc tcgccatgct taccggtgac aagcgggctt
     6121 ccgccaatat cattgccagc gaagtcggca ttgacgaggt tcacgccgaa ctgttcccgg
     6181 aggataaggt agccgcagtc aaggccgcta caggagccgg caaaaccgtc acgatgatgg
     6241 tcggtgatgg tgtgaacgat gccccggtgc tcgccgtcgc cgatatcggt gtggccatga
     6301 ccgacggcac atccaccgcc gcttccgaaa gcgctcaggt ggtcattatg aatgacaata
     6361 tcgccgccgt gccgcgcgcc atcgccattg cacgccgcac caagcgcgtg atgcttcaag
     6421 ccgtaatcgc gggccttgtg cttgccacca tcggcatgat tgccgccgcg ttcgatctca
     6481 ttccggtcgt cgtcggcgca ttcctccagg aggccatcga cgtggtgagc atcctctggg
     6541 ctctcactgc tttgattgat cgggactgat taagataaac cggaattgat tggagccgca
     6601 tccggattat gtgcaatata acaatttgat atcaatattt tgtgtgtgct taccggatgc
     6661 gggtgccgta ggtggcagtc tattagacta aaggctgttt gcagcatcat ttaggggcat
     6721 tgaggccttt gccagaggag tcgatatgtc agagggtgag gaagagctca atgtgggcgc
     6781 agtccgtttg gaggaagtgg catcggcggc taatgtatcg ctgtccactg cgtccaaggc
     6841 cttgcgtgga aagcctcacg tcagcgagaa aacccgtatt cgagtgattg atgctgcgcg
     6901 gaggttgggt tatagcgccg cagaagctat ggcatcgatg gcaaggttaa gcgtcaggga
     6961 ttctcacggc atgcaggatt ttcgcgttgg catcattggc gttgatatga aaggcgtctt
     7021 ttctccgacc attcttattg gagcggagaa tgcgtttcaa gcgcgctcag ccgcagcgct
     7081 actgttcaat gcgtcaggca atgaggcatt gttccacagt ggtattgaac gaatgttggt
     7141 gcaaggagtg tcgggactgt tgattatcag caggtgtacg gatccggtgc catactacgt
     7201 ggattctccg gtacctgtgg tctacgccta tggcccgtcc cgtgatcccg atgattgttc
     7261 cgtggcacaa gacaatgtgg aatcagggtg tttggccgtt cgccaccttc tttcctgcgg
     7321 gcgcactcgt attgcgatta ttgctggcga ctccgagtac tcggttacac atgaacggat
     7381 gcggggtgcg ctcaaggcct tgcatgatgc tggattacag ttggctggcg aagcgcggtt
     7441 cggtccatgg gaagcggaat gggggcgcgc cgctgcccgt ctgctgctca atgacggtgc
     7501 tgcattcgac gcagtgatat gccaaaacga tgtgctggct cgtggctgca tcgaggaatt
     7561 ggaacgtaac ggatgtcgcg tgccgcaaga cgtagcggtt atcgggcacg acaacagtcc
     7621 ggattatgtg tcgtacgcgc atccgttgct tacttctatt gatgacaaca acgaaatgct
     7681 ggggcgtcaa gctgccctat gcctactgga tgcgattgcg gggcatcctc accatggtgt
     7741 ggagcgtgtg ccggtgaagc taatccaacg cgagtcaacg ttgccgttgg attgataacg
     7801 tcaccggata ggcggcagca gccggttcac atcttgcgtg gcatcatcca gacctttgcg
     7861 aatatcattg atgccggaat cctgtacaag gccgtgaacg tcgatgttgc tttgttcgct
     7921 caacgcttgg ccgcgtacgc gcatgctatg gataaatcgt ccgaccttgc gtgcgtagtc
     7981 gggcagcttg tcggggccta ccagcactaa agcgatgaca agaatcacca tgagttccgt
     8041 gcccgaaatg ccgaacaaca tattattctc cttctgcagt tattgttgca ggggcttcct
     8101 gtttgccggt gcgcttggct ttgcgcgccg cgcgaaatgg taacgcggcg cgcattttat
     8161 gtaaaagttt gagaagtttg ggttcttcgc ccgccgcgcg catgactttc catagctgga
     8221 tgtgctcgta tactgcgcat acggctatgg ctgcgaaata gattgcgatg atgggcaaag
     8281 cgagtgcgca taatgttcct gggtcgccgg ttggcgtggc cacggccgcg aagataaacg
     8341 ctatgaccgt tgcgagacgc cactgcttca accatgttcg tgtttcgacc gcattcatca
     8401 tcgtcaatgc cgccataact acgggtagca ggaatgagag gccgaaagcg actgtcatgc
     8461 gcagaatgaa atcaaaatag acgtcggcgc tgagcaatgt ggcggtgttt gcgggggcaa
     8521 agccggtaag gatcgccacc gcttggggca gcagatacca tgccagtccg gctccggtga
     8581 ggaacagcgg cactgaggct ccgatgaatg taaccgcggt ccagcgctct ttgcgcttca
     8641 accctggatt aatgaatgcc cacacttggt atgaccacca cgggcaggag gccgtcagac
     8701 caaggaagaa cgcaatctcc agctttacgt tgaatgcgga gaccacgcca ttgaatgtga
     8761 tgctcatcag tccctgttgc tgcccggccg ccgtcaggaa cggttgttgc aggatacgaa
     8821 acacctgatc gctgaacatc catcccacta tcgacatgat gatgatgccg gcaatggccc
     8881 gaacaaagcg tttgcgaaat tcgacaagat gatcggccag aggcatcacc gcatcggggt
     8941 tgcggggctt gcgcgttttg gacttgcgcc ctacaccccg cttccaccgg cgtgtagggt
     9001 gttgcgcacc cgcgctggcc tgctgcgtgg cctcgtgtga gtgatcgatg agatcgtcga
     9061 ggtcaagatg tgtatcccga tcgccgggat gcatcgccgg tgcaaggggt gccggaagcg
     9121 cggggtttcc gggaaccgtg ctcatcgctg ttcggctccg gttgctgtag tcggggcggc
     9181 ggtggcgttc atcgtgacgg cttggggtac ggcgggagcg ggagccggtg ctgccgccgg
     9241 agctggggca ggggcgtcgc tgactgtatt gacggtttgt tgtgttgccg ggtcatcggt
     9301 cttggtctcg gcacggaaaa tcttcatcga ttggccaatg cttttggcta gctcagggag
     9361 cttcttcgcg ccaaacagca gcacgatgat aagtagaatg ataatgagct cggtggggcg
     9421 aagattaggc atgatagtat gtccttccaa tttctgtgtt gtgacagggg aagggtatac
     9481 ctcattaacg caccaattta tcagtatagc gaggaatggt gtgttgttca agttgaagtg
     9541 cggtgttgcg tttgagctgc ttgtgagagt tgctcgggaa catgttaatt tgaatgcatt
     9601 tactgtgtta ttggtgagca tatgtgtttg ggttcgttgg ttttcgcaag aggaaaacaa
     9661 aaggaaaatt acgaaaatag catggctgga aatgctgata aagactgatt ttcattggta
     9721 ttgtgcgcta cgaaaaccag agaaaaaatg agtgagtcgc tttctttgac gcatggtgag
     9781 aatcgtctgt ttgagttatt gtgaacgcgc acaacgaaga caagcggcat ggagggcgcg
     9841 caaaaactcg aataatcgca tatggtaact gtttgcttta ccgtcatctc tcccatacat
     9901 acatgacgac ggggacgcac cacaatgatg tgatggtaaa acgggcagta ccagacgatt
     9961 ccgagtatgt gtgtgccatc cactgacgca tcttacctgc gtcgtcgggc cattgaatgg
    10021 attaagtgca ttgaatggtt gcgaatgcgg cagagtgtct tcgtccgtca tgcctcgcaa
    10081 tgaacgcgcg gcagtccaac gaaggagcaa gatgagttca acggaagcaa caaacggcat
    10141 ctctcgccgt gctgtgctcg caggcgcggc cgccatcggc ggcaccatcg ccgtggcgag
    10201 tgcattgnnn nnnnnnnagt gcattgggta caggagtgac aacggtgcag gcagcgccat
    10261 ccgtcgctgg cacggcaggt gcaacgggcg cgaacggcgc gggcggcgtg gccgttcccg
    10321 cagcgcagcg caccacgaaa agcgcgaaga ccattaccga cgcgcccgct ctgccgggct
    10381 ggaaggtagc tccgttccct ctgcggaacg tcgccatcac ctcgaacagc gtgttcgatc
    10441 gtgccaagga agggatgctc gactatgcac gcaactatcc ggtagaccgc tggctggttt
    10501 gcttccgtgc gcaggcgaac ctgctgccta aggacaacac gacgcagcct tccggtggct
    10561 gggagaactt ccccagcggc tcgctagaca aggctgttga acagcaatgg ggcgatgccg
    10621 aatacacgcg tggccagaac aagaacggtg ctgatggtct gttgcgcggc cactttgccg
    10681 gacatgcatt gcacatgctc tcccaagcgt atgccgaaac gggggaggaa gcgattctca
    10741 acaagatcaa cgagttcgtc tccggcctga aggaatgccg agattccctg cgcgagatga
    10801 agtataacgg caaagcccgc tacagccacc ccggcttcct tgccgcctat ggcgagtggc
    10861 agttcaaggc attggaggaa tatgctccct atggcgagat ttgggcaccg tggtacacgg
    10921 agcacaagat tcttgccggt ctgattgccg cttacgagtt tgccggtaat gccgacgcgc
    10981 tcgacctggc tgaaggcatc ggacattgga cctatgcgcg tctgagcaaa tgcaccaaaa
    11041 cccagctgca gaagatgtgg gacatctaca tcggtggtga atacggcggc atgaatgatt
    11101 cgctggtgga tttgtacaac gtctccaagg acaaggaccg tagcgaattt cttaaggcct
    11161 ccgcattttt cgacactgac aagctcattg acaactgtgg tgctggtgtc gatatcctca
    11221 acaacttgca cgccaaccan nnnnnnnnna ttccacagtt cgtcggttac gccaaagacg
    11281 cggccatggg cgacgcggat atcgatgctg atgctcgtgc gcgttatctc aaggccgttg
    11341 aggggtactg gggcatgatt gtgcccggcc gtatgtatgc tcacggtggt actggcgaag
    11401 gcgagatgtg ggggccggca catactgtgg ccggcgatat cggcaagcgc aacgcggaat
    11461 cctgcgcggc atacaacatg ctcaaggtgg ctcgctatct gttctttatc gaacaaaaac
    11521 ctgcctacat ggactattat gagcgcacga ttctcaacca tattctnnnn nnnnnngacc
    11581 tcgattccgg cacggccctc acgccgggca actgctacat gtacccagtg aatccggcta
    11641 cgcagaagga atatggtgat ggcaacatcg gcacttgctg tggcggcacc gctttggaaa
    11701 gccactcgaa gtaccaggat tccatctact tccactcgac ggacaacaag gaactgtacg
    11761 tcaatctgtt cacagcttcc acactcgact ggaccgacac cggcctgaag ctcgcacagg
    11821 aaaccaacta tccggaggag gagacctcga cgatttccat tacggccgcg ccgaagagcg
    11881 ccgtcacctt ccgcatccgc atccccgcgt ggagcaaagg tgcgaagatc gaggtgaacg
    11941 gcaaggctat agatggcgtc accgctggcg aatacgccac cgtggccggc agctggaagg
    12001 tcggggacaa gatcgtcgtc accatcccgc tgcagctgcg caccgagtcg actgatgacc
    12061 gcaaggacat ccagacgctg ttctacggtc ccacggtgct caacgcgctc aacccatcca
    12121 ccaagttcat ccaacgcggc ttctacgagc gcaacggctt ggatggcacc atcaaactgg
    12181 gcgtacagaa gaaggaaggc acgaagaact acttcatcat tgacggcgac gagttcgagc
    12241 ccgcctacaa cggcgacaac accccgtacc acatgtactt ccagcgcaag gacgagtatg
    12301 tgggcttcgc aggcaagctc accgatgtgc tgaacccgaa gcgcccggct gcacaggacc
    12361 gtagcgagag cacgctgatg gatgacatct gggcgggcgc gccgttcaag gaccgtgccg
    12421 ccttcatcaa gaaggtgcag gaggtcacga agacctatca agacgagggg ctgctttcca
    12481 gtcgtgatcg tcaggtggtt ctgcttgcct ccgctcgcgc caagatgagc tgacgttttc
    12541 ctgcgaccaa accgtctccc gtagtgcacg acacggcggg agatgagtga tacaaaccat
    12601 aacgcctaac agaaagaata ttatgaccat cgaaagcact acttcggccg tgaagggcgg
    12661 tgtggcgaaa cgattcgccg cactgttcgt agctgcggcc acgctgctgg ctggcggtgt
    12721 cttctccgta tcggccgcgc cgagtgcact ggcggacgac gctgaaacga atgttgcgct
    12781 caatgcagaa aaaggcaacg acaatgcacc agacgttcag gtcagctatg tcactggctg
    12841 gaacagcact gctgccatca atgacgatag cttggacggc aacgccagct atggcggttg
    12901 gggcacttgg ggcaacacca gtgccagcga aactgccact tacacatgga atcgcgccgt
    12961 gaccacgtcc aagtctgtgg gctactactg gacgaacgtc aagaccggtg atggtggcgt
    13021 gccgctgccc aaggatgtct cgattgagta tctcaatgct gattccggcc agtacgagac
    13081 cgtgccgaac ctgaaggtgg acaagacatt ccccgccgac gacgagctcg acgccaccaa
    13141 cccggtgtat ggtccgtata cctacacctt cgaccaggtc accaccaagt ccatgaagct
    13201 tgtggtgaac aagaaggcca atgacaacaa gggtattacc gtcactgagt ggcaggtgtt
    13261 tggtaccagc gctgcactac cagtggatcc gggcgaccca ggtgctttct tagacgtgca
    13321 gcaagttgcc gtgcgcacca cgccgggcgt ggacccgacc tcgaagttgc ctgcagaagt
    13381 gtgggcgaca cctcaagatg gtcccagcat taaggttcca gtgacttggg aaaccgttcc
    13441 ggccgacgct tatgctacag ctggtactac cgccgaagtc aagggcacta ctgctgccgg
    13501 tacctatgac aacatcaaag ttgcggccgg tcaggcgacg gccactattg gtgtctacga
    13561 ccagctcaac accgaggtga ccgacgcgga gtacgtctcc accatcaccg ctcccggtgt
    13621 caagccggta ttgcaggata ccgtggcgct gacctacgct gacggctcgg tcgagtcggg
    13681 tcacaaagtg acttgggaca gtatcccggc cgccgagtat gctgaagccg aacaggtcgg
    13741 cttcgtggaa ggctctatcg agggtgtcgg caatatcaag gcggaaggca cgttcttcgt
    13801 cgtcgcgcct tccgacccga aagcccagcc gattgttgct atcgatttcg actccgcggc
    13861 ccccggctcc ggctggtaca ccactgcccc gaagttcacc atcaccgcgc agcgcggcgt
    13921 ctccaccgtg ccgataaaga cgatcgagta caagatcggc aagggcgagt ggaccagtgg
    13981 caagggcccg atcagcgtga ccgcgcaggg ttccgtgacc atttccgccc gtgccaccga
    14041 tgaaaacgac aacagccgcg aggtctccca gaccatcaag gtcgacacca acgtgccggt
    14101 ggtcaccgct aagcaggaga gcaagaacgg caacgatgtg gtcgtctctc tcaccgctga
    14161 tgacggtgcc gcaggctccg gcgtgacccg tgtgctgtac tccaccggcc cgtccagcaa
    14221 cccgaagtcc aacgagaaca cgatgtgggg cacctacacc gacgaggaca aggtgaaggt
    14281 gcagctctcc gctgaccacg acacttacgt gcacttctat gcgcaggatg ccgccggaaa
    14341 tgagtccgac tacgccaaca tcaatctcgg caagggcgaa gccgtgaccc cgaccccgac
    14401 tccgtctgtg gattccgttg ccgtctccgg tgatggtgtc aaggatggca agctggcgct
    14461 ggtcgctggt gatactgccc agttgtcggc cgaggtgaag gtgaccggtg atgcggccaa
    14521 gaccgtggcc tggaagtcct ccgacgcgaa ggtcgctact gtggacaagg atggcaaggt
    14581 gaccgccgtt gccgaaggta cggcgaccat caccgccacc tccacggttg acgagaacaa
    14641 gtccgcgtcc gtcgaagtca cggtaactaa gcccgaggct ccggttccgc ctgccaccga
    14701 tgaggagcgc aagcctcttg ccgacaagat cgccgctgtt gagagtgaga agctcaacca
    14761 gaacgactac acggctgatt cgtggaaggc atatgcggat gcacttgctg ccgcaaagca
    14821 ggtagtcaag gatccggccg caaccgcacc gcaggtgacc gctgcgctca acaaactcac
    14881 tgccgcacac gatggtctcg ccaagaagga tagcggcgaa aagcctgaac cgcagaagcc
    14941 cggtcagcag aagcctgggc aaaaccagaa gcctgaccag cagcagaaga acgctactac
    15001 cactgccccc acgggtagtg cggtggctgc cgttgctgcg gccgtagttg tgctcggcgc
    15061 ggctggcgcc ggtctggtta tctggcgcaa gcgccgtgcc tgacgtgagg ctattgcccc
    15121 taggctaata acaacccaat aattgaatac aggtcttata cctgaagttg atctgcgtcg
    15181 cactggcttg aacccgaatc gatgttcggg ctaagcctgt gcggcgcatt tcgttgcggc
    15241 tgtgttgctc ataatccggt atcggagtta ttcattaggt cggtttgacg tttattaggt
    15301 cggtccttgg tcggagcggc ccttgaaacc ccgccgttcc aagggatggc ttcagcaccc
    15361 tccgacctaa tgaacgaaga aatgacctaa tgaatgcaat cgaggtcgta aagagccgtt
    15421 ggaggtgaca tcttcagtgt catcaaggcc cgaattcaat ttgtaagtgc aacgctcgtc
    15481 tttgggtggc ttgtcttcga gagtaccttg ccgagctctc gataccaaac ctcgttgggc
    15541 gtttcccagt ccaggacctt catgggggtg tcgttgatct cgtccacgat ggcctgcagg
    15601 tcctcgtccg tcaggccgtc gaaactggtt ttcttgggca ggtagcggcg gatgcgtccg
    15661 ttgcggttct cgttcgtgcc gcgctggtat gaggagtagg ggtccgcatg gtaggtgagc
    15721 atgccgaggg cctcgtccac gagcaggtgg caggaggact ccgtgccgtt gtcccaggtg
    15781 cggtcgatgc gcgcgggcgc ggggatgtcc ttgtagatgt cgtattcggc gcgcgccgtg
    15841 gccggcgcgc tcttgtcggg gatgaggcgg gcgaacaggc ggcggctctt gcgttcgacc
    15901 tgcgtgtcga tgcaccgttt cgagggggcc gtgccgatga cggtgtcgga ctcgaaatgg
    15961 ccgaactcat gccgcgagtc gacacgtttg ggccgctggg cgatgggcac gcgcatcggg
    16021 atgcgcggcc ccttcgacct cctgcccttg gcgcgcgtgc ggcgccgctt gcctctgggc
    16081 aggtactgcc tgagatccag gatcctctgc ggcttggcgt agatccactg gtagaggcac
    16141 tcgtggctga ccctcatgcg cgggtcgtcg gggaactcga ccgcgagccg tccctcgatg
    16201 cgttcgggcg tccatccctt gcgcagcgcg gtcatgaccc atgcgagcag cgggtcgtac
    16261 gccatgcgcc tgggcttgcg cggttcacgc gcgcgttgtt cggccttgcg ctgcgcggtc
    16321 atcgccgaat agaacggacg gcacgtccac ggccccgtct tcaagcgttt cggccggtac
    16381 ggccggtacg agtcgttctc attggacgcg aaccacaatc cgcgtttcag ctcgcggctg
    16441 accgtggcct tgtcgcggcc gatcctcagg gctatctggc gcacgccgag cccgtccctg
    16501 ttgcgcaatt cgtcgatccg gatccgttcc tctgccgaca ggtgggaata agctttcata
    16561 tcgagcgcag cactttctct tcggttgtgg acttgaacac ttccaaccat agagcgggtg
    16621 ttgcgcttct atttagaact caggagggat tcgccggttg tgcagtattg ttattgttct
    16681 gcggcggctc tttaccgtcg gccttccagc caatcgaggg cggaagtgac gcggattcct
    16741 tgtgagacgt aactttgctc aacccgactg gttacgtact gttgctcaca cggtatgccg
    16801 agaacattgc caattaaagt aaggtgccgg gcgaaccggt catggtaggt ttgcccggat
    16861 ttgatttcga tcatcaggcg atgattcgga tcggtgaaat ccaacaaatc cacctcgacc
    16921 ttgctggcat cgcggtagaa gaataattgt ggctcctgat tggcgttcaa atgctgtttc
    16981 atggtttccg caatgatgag gttttcaaat atatcgccga acttggggtg cgcaaccagt
    17041 gcttcgaccg tccgaatgcc caagagatgg cacagcaagc cagtgtcata aaaatagagt
    17101 ttcggcgttt tgaccacctg tttgcgtgcg ttcgtgtaga acggcatgag atggaatgcg
    17161 atgtaacttg attccagaat cgacatccat gcctttacgg ttcgcggact tacaccaagt
    17221 tcattggcga tgttggtgta gtttatgagt gctccggaac tgagtgcgca aagagtcaag
    17281 aatcggcgga agtcagccaa attgcgtaca tcaagatatt cggaaacatc acgttcgata
    17341 taagtgtcga tgtagttcga gaagaacaga ttgagcggca ttctcgtgtc gtaaatgcgt
    17401 ggataaccgc cttgcagcat aaacgtatcc ggagtaaggg cctgattcgc cttgcatgct
    17461 tcttggaatg agaacggcaa taacttgacc aatagggaaa cccataatct gacattagca
    17521 gtgctcgtag gtttcccaaa cgcacaaact atggcctaat acacagaatt cccaccaccc
    17581 tccctctgtc gcaacggaag gctttgcgtt aggctagagc gcgtgactga atccgtgaat
    17641 actgaactcc gcattgctgt aatcggcgct ggcccggccg gcgtctactc atccgatatc
    17701 ttcctgcgcc agctcaagaa gctgggcgaa gaacttggcc tcggcaccga agcgcgcatc
    17761 gacctgttcg agaagctgcc ggtgccgttc ggcctggtgc gttacggtgt ggccccggac
    17821 cacccgtcca tcaagttcat cgcctccgcg cttgagaaga cgctcgacaa cccgaacatc
    17881 cacctctact gcgacgtgga attcggcaag gacgtgaccc ttgacgacct gttggcccgt
    17941 tacgacgccg tgctgttcgc caccggcgcg gtcaaggaca agccgctgaa cctgcccggt
    18001 gccgaccttg aaggcgtgta cggcgccgcc aagttcgtcg aatggtatga cggctacccc
    18061 accggtgccc gcgaatggcc gcttacggcc gagaacgtcg ccgtaatcgg cggcggcaac
    18121 gtggccatgg acgtggcccg cgaactcatg cgcaacgccg acgacctcaa ggtcaagacc
    18181 gacattccgg acaacgtgta cgagggcatc ggccagaaca aggccaaggt gctccacctg
    18241 ttcatccgcc gtggcgtggc ccaggccaag ttcagcgtgc aggaactgcg cgagatggag
    18301 aagctgcccg gcgtgcagct catcatcaac gaggacgact tcgatctgga cgacgagacc
    18361 atcgaggtgg ccggcaagga caagctcacc cgccagatgg tcgaggagct gttcgccatc
    18421 cgcgagatgg ccgaagacat ggaggatgac ggcgacgtcg acttcgaagg caacccggcc
    18481 gaccgcaagt actacgtgca cttcaactcc gcccccaccg agatcctcgg cgaggacggc
    18541 aaggtcaagg ccatccgcgt ggagcgcacc gaaaccggtg ccgacggcaa gatgcaccgc
    18601 accggcgagt tcaccgacta cccggtcgag gccgtctacc acgccatcgg ctacaagccc
    18661 gccgaagctc ccggtatcac ctacgacgag cacggcgccc acctggccaa cgccaacggc
    18721 gacggccgca tcaccaccga ggccgacggc ggcgaagtgc gcgaccgcct gtacgccacc
    18781 ggttgggcca agcgcggccc ggtgggcctg atcggctcca ccaagtccga tgcgctcgcc
    18841 atcgtgacca acatgctcga ggatctggcc aaggccgctg aaggtggccg tgtggcagcc
    18901 gaccgcgacc ccgaatccat cgaccgactg ctggccgagc gcggcattcg cccgatcgac
    18961 ttcgccggct ggaagaaggt cgacgccttc gagcgcgccg agggtgccaa ggaaggccgc
    19021 gagcacaaga aggtcatcga tcccgaccag atgcgtgagc tcgccctggc ctgacgttcg
    19081 cccacgcccg tccggtattc gtccacccgg ccgcgccccg tgcggacgcg gccgggctcg
    19141 gggccggacg ggccgggcga atatgggaca tttctcgcgg cgcatatcca gcaatcattc
    19201 agcctgcgat tttagtgtgt gcattatgga tgccaaagta cgggtgcacg gccatttcaa
    19261 cggcctgaaa acaacgctgc tgtttgccct gatgtgggcc atcatcatgc tgatttggtg
    19321 ggccacgggc ggcagtcggc aaaccctgag catctatatc gtcatcggcc tgattaccac
    19381 gttcggcacc tactggttct ccgacaaact cgccatcgcc tccatgggcg cgcgggaagt
    19441 cagcgagcag gaagccccgg agatctacca gattgtgcgt gaactgagcg ccaaggccgg
    19501 caagccgatg cctcgcatct acatcgcccc caccatgagc ccgaacgcct tcgccaccgg
    19561 gcgcaacgaa cggcacgcgg ccgtctgctg tacccaaggc atcctgcaga ttctcaacgc
    19621 ccgtgaactg cgtggtgtgc tgggccacga acttatgcac gtgtacaacc acgacatcct
    19681 gacttccgcc atcgcctctg ccatggccac cgtcatctcc tacctaggct attcgctgat
    19741 gtatttcggc ggcggttcgc gagacgaccg cgacagttcc ggtggcctcg gcctgatcgg
    19801 cgcattgctg agcgtgattc tcgcgccgat tgccgcgtct ttgatccaga tggccatctc
    19861 ccgcacccgc gagtacgacg ccgatgaaga cggcagcctg cttaccggcg atcccgaggc
    19921 cctggcttcc gcactgaaca agatttccta cggcgcgcag accacgccga tgcgcaaaac
    19981 cgccggcacc cagtccgtct ccgcaatgat gatcgccaac ccgttctccg cggtcggctt
    20041 ctcgcgcttg ttctccacac atccgccgac cgatgagcgc atcgcgcgcc tcatgcagat
    20101 ggccaacgaa atgaacggca cgccgatcgc tccgcccacc tactccacgc gcgtcggcag
    20161 gtgacgtgct aggataacta ctcgtacctc gtttggctga gcatgctcgt cacgtgaggt
    20221 gcagcagccg taaggcatgc ggacatagtt catcggtaga atgaaagctt cccaagcttt
    20281 agaggcgggt tcgactcccg ttgtccgctc cattgatttt caatgatttt cattatttcc
    20341 gggaaagtgt cgcgcatgat cgcaaaacta cgcgctattt gagcgctttg cccgcggaac
    20401 ccagctgccg gcaagcctcg accacgcggg cggacatgga atcctcggcc ttacggcccc
    20461 aagaacgcgg atcgtagaac ttcttctcgc ccacttcgcc gtcgattttc agcaccttgt
    20521 cgtagttctc gaacacgtgg tcggcgattg gacgggtgaa ggcgtactgg gtatcggtgt
    20581 cgatgttcat cttgatcacg ccgtagctta cagcttcggc gatttcttcc gggcgtgaac
    20641 cggagccgcc gtggaacacc agctcaaacg gcttgccgtt ggggaaatcg actatgccgg
    20701 ccgcgctagg caactcgcct tcggccaccg cgcgcgccac acgcgcctga atgtcgccga
    20761 gtagcgacgg acgcagcttg accacgccgg gcttgtaggc accatgtaca ttgccgaatg
    20821 tgaaagcggc catgtaccgg ccacgttcgc ccaaacccag tcggcgggcg acttcgaggc
    20881 catcttccgg agtggaatac agcttttcat tgatttcggc ggagtgaccg tcttcctcac
    20941 cgccgaccgc gccgatttcg atttcgagca cggtgtgcgc cttctgcgac ttgtcgagca
    21001 gttcctcggc gatgtccaga ttttccttga gcggcacggt ggagccatcc cacatatgcg
    21061 actggaatgt aggctcctgg cctcgtgcca cctgatcggc ctcatgcgcg agcagaggac
    21121 gcgcccactc atccagatac tgcttggcgc agtggtcggt gtgcagggcg atggtgatgt
    21181 tcgggtattt cgcagccact tcatgtgcga aagccgccag tgccagcgag ccggtaacgc
    21241 gatcattgac ccctatgccg gagacgtaag ccgcgcctcc cacggaaatc tggatgatgc
    21301 cgtcggactc ggcgtctgcg aagcccttca atgcggcatt cagcgtctgt gagctggtta
    21361 cgttgatggc tggataggcg taaccgcctc ggcgggcggc atcgagcatt tcggcatagc
    21421 gttccggtgt tgcgatcgtc atggcttcat tatcgcgcga ggcatgccct ctaggccccg
    21481 ttcttcaacc tgtgcgctgt gcgattggtc acagcatcgc ctcgtacgag tagaaattcg
    21541 tcacccattc cgggttgtcg cacctccagc ctcgccctgc gtgagacgaa acttcgcagg
    21601 gtagagggtg tacggtagtc tgtttccggc ggaatcgtgc gaatgcggag gtgcatcatg
    21661 cctggaattg ttcttattgg tgctcagtgg ggcgacgagg gtaaaggcaa ggctaccgat
    21721 ttgatcggca ccaaggtcga ttacgtcgcg cgcttcaacg gcggcaacaa cgcgggccat
    21781 agcgtggtgg tcggcgatga gtcgtacgcc ctgcacctgc tgccttccgg tattatcaac
    21841 ccgaacctga ccccggtgat cggcaacggc gtggtcgtgg acccggaagt gctcttcgag
    21901 gagattgacg gcctggaatc ccgcggcatc gattgctccc acctcaaggt gagcgaggcc
    21961 gcacacatca tcgcgcccta ccaccgcaca ctcgacaagg tgaccgaacg cttccttggc
    22021 aagcacaaga tcggcaccac cggccgtggc atcggcccgg cctacgcgga caagatcaac
    22081 cgtgtcggca tccgcgtgca tgacctgttc aacgccgacc atctgcacga caaggtggag
    22141 gccagcctac accagaagaa ccagatgctg gtcaagctct acaaccgtcg tccgattgac
    22201 gtggaccaga ccaccgagga gctgctgaag ctcggcgaac gcctcaagcc gtacgtggcc
    22261 aacaccggcc tgattctcaa caaggcgctt gacgaaggca agaccgtact gttcgaaggc
    22321 gctcaggcca ccatgcttga cgtggaccac ggcacctacc cgttcgtcac ctcttccaac
    22381 ccgaccgccg gcggcgcctg caccggcacc ggcgtgggcc ccaccaggat cacccgcgta
    22441 gtcggcgtct ccaaggccta cgtcactcgc gtgggcgaag gcccattccc caccgaactg
    22501 ctggacgagt ccggcgaatg gctgcgtcag cagggccacg aattcggcgt gaccaccggc
    22561 cgcccgcgtc gctgtggttg gttcgatgca gtcgtcaacc ggtacgcctc ccaggtcaac
    22621 ggcctgaccg acatcgtgct caccaagctt gacgtgctcg ccggtctgaa ggagattccg
    22681 atctgcgtgg cttacgacgt ggacggcgaa cgtcacgacg atatgccgac cgatcaggcc
    22741 gctttcgccg ccgccaagcc gatttacgag accatgcccg gctgggatga agacatctcc
    22801 aactgccact ccttcgacga gctgcctgcc acctgccagt cctacgtcaa gcgtttggaa
    22861 gagctgagcg gctgccgtat ctccgcgatc ggcaccggtc cgcagcgcga tcacatcatt
    22921 tccgtgcatt cgctgatcga ctgaccgtct gcaacgaatt tcccggcttg actggtaacg
    22981 acgcggcaca tgatatccgc cggctgattc cgcgattcga ccacttgaag t
//
LOCUS       DAWBMW010000011        22913 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_612_length_22913_cov_3.006212, whole genome shotgun sequence.
ACCESSION   DAWBMW010000011 DAWBMW010000000
VERSION     DAWBMW010000011.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 22913)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 22913)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..22913
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_612_length_22913_cov_3.006212"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    14650..14659
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    14992..15001
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4166 a         6666 c         6935 g         5126 t
ORIGIN      
        1 atgtgggtga gttgaggtcg atattcgggt aaagttgcgc cagtagattg ttggcctgtt
       61 gaatcatggg cagcatctga tcaatttgcg ccatgccttg gtcgatttgg gtaatgccct
      121 gttcgatttt cgtgcgattg gcagtcagtt ccgtttgcgc cgcatcgagc tgtttgcgag
      181 ccgaggtgag ctgcgctttg cccgattcga gctgggtgcg cgcttcggct atctgctggc
      241 gaccttgcgc gatctgttga cgggccgatg caatctgcgc acgtccatcg gcgagctgcc
      301 gttcgccatc ggtaatggtt gtcctgttgt tctccaattc ggtacggcta tcttgcaatg
      361 tcgtttcgtt ggcttccagc tcggcccagt tgtcgtcgat ctgcttctgg gcttcgtcca
      421 gctgctcgtt ggcctcgtct tgggcatcgt ccagtttgcg ctgcgcgctg gagacgatct
      481 gttgacgacg ggctttctgg cgtgtggtct gtatctgatg ttcgatacgg tcggccacgg
      541 ttttgacggc ttcatcgtag gcatcggaaa acgtgtcaaa atcggacgca ccagtgacgg
      601 caaccgatat cgcagtgtat atattgccgg tcacgccgtc gctgggtgca aagaacgtat
      661 agtcctcgga tgaggtggac cggaaggagg tcatgcccga atagccgtct ggattgttga
      721 ggtcgcgcgg gtcaagtacg acgccggtga tggtgagttt cgtggggaac tgcggggatt
      781 gctcggcgtc ggcgtccgta ttggcggcgg acttcgcatc ggatgccgag gcattggcgg
      841 cgacacctac cgtatcggca ttattggaat cgtcagtctc cgtggccgct accgatatga
      901 cggatgaaga ggtgtcttgt ggcgtcacag taatggtgcc gccaatcttg agtccgctat
      961 cgttcaggaa ctgctgagtc accgccactt cgccggcctt gttcggcagt ttgccttgtt
     1021 gcaggtacgg ctgatcaagc ccttcggtac cgatttcctg catggtcacg gttttcttgg
     1081 tgccgtccac caacgtggtc accgtctgag aacgttcggg ctgcacggtt tgcacaccgt
     1141 cgattcggcg cagtgccgtt gcatcatcat cggtcaggcc gtatgtggat agcacctgca
     1201 ggtcatgcag tccctgctga tcgtaaaaac gaccggccgc cagaaaagca tcgcggcatc
     1261 cggcgtagat gccggtcagc acggccacgc caagcagcgt gatgacggcg atcgagacga
     1321 acctcttcca gctgcgcagc cagcaacgca acgtatcttt ggcaaatacc gcagaaacgc
     1381 ccgctcctcg accgccgcta tcgtcgaggt tccgccgttt cacagaatgt ttcacaggtt
     1441 gtttcacgaa tcgcctcgct tgtttcacgg ggcgtttcac agagtgtctt gcagtgagca
     1501 tcgccaatca cctaccattc gatatcggcg atgggccgcg gcgttgcgtt gacctcctcg
     1561 ctggtcacct tgccggagcg gaagcgaatc actttgtgag ccatcggtgc aagcgcggaa
     1621 ttatgcgtga tgatcattac ggtcatgcct tgtgtttggc aaatatcctg cagcaactgc
     1681 agcacttcct tgccggtctc gtaatcgagc gcaccggtcg gctcatcgca caacaacagc
     1741 ttcggctttt tggcaatcgc gcgggcgatg gatacacgct gttgttcgcc gccggacagt
     1801 tgcgcgggga agttgtccat cctctccccc agtccgactt tgcgcagcgt ttcccctgga
     1861 tcgaaatggt cagggcagat ttgcgaggcg agctccacgt tttcgagcgc ggtaaggtta
     1921 ggcaccagat tgtagaactg gaagacgaag ccgatatcgt tgcgccggta ggtcaccagt
     1981 tcctttttcc gcagacccgt aatatcgcgt ccgccgacca ctacgcggcc ggttgtggcg
     2041 gtatccatgc cgcccagaat gttcaatgcc gtggttttgc cggcaccgga ctgtcccaga
     2101 atcacgctta attcgccttg ttcggcggta aagctggcct cgtcgagcgc gcgaatcaca
     2161 ctgctaccag aaggatattc cttgaccaca tggtcgaatt cgatatacgc catcgcacac
     2221 ctccaacgcg ctcgtggcag ccacctagcc ccataggccc cgccaaaact tcctgctata
     2281 ctcaagtaaa acaacactgt gtcggttctg caaccaacag ttggattgtg ggttgtcgga
     2341 atttggggag aaggccatgg ccaaagaatt gacggaaagc cgccgcaccc gctacacgag
     2401 acgtgccatg caggacgcgc ttatcgagtt gctgcgcaat cagccgctcg gctccatcac
     2461 cgttaaagcg ttgtgcgagc gggccgatgt gaatcgttcc acgttttacg cgcattatgc
     2521 gagcatcgag gaactgctgc acgacattga agacgaaacc atggcctggg tcaccaccgc
     2581 actcgaacag ctactcgccc agccagattc tgccggcatc gagcatgtta ttgagcgcat
     2641 ctgccagtac attgccgaca atcgcaagca tctgcaggta ttgatgagcc cgaaagcgga
     2701 tatcggcttc cagcagcagc tgctgggatt gatttacagc cagcgcggcg taggcgaaca
     2761 gttgcaatct tcggcgggtt acccagccga ggcgcagatg cgtatgcgat tcgccgtgtc
     2821 cggcagcatt gggcttttgc aatattggct cgccaccgat ctcgcggcct ctcccgaatc
     2881 ggtgtcccac accatcttca ccatgtgtat gcctgccacg cagtgaacac ggtgaattca
     2941 gtgaactcag tgaagcgtgc ggtttcataa gcatcgctcg ctatagcttc gcctgctcac
     3001 cggccgcgcc cacgcccgcg cttgcgatag cgcacaaata gacgattgac catttctgag
     3061 acgatacggc ggcatagaat aacgaacgtt gcgcatcgcg gcaagccccg cgtgcgccaa
     3121 aacccaagaa atgtgagaga agggcacaat gagtactgct gatgcgccta agaagccgcg
     3181 cgaaaccgac gacgtgccgg tgccggccac gctgcgcaag tcactgaaga accgtcatat
     3241 ccagctgatt gcactgggcg gcgccatcgg aaccggcctg ttctatggtt ccagcgaatc
     3301 catcgcgctg gccggcccct cgattctgct cgcctacttg gttggcggct tcgcaatctt
     3361 catgatcgtg cgcgcgctga gcgaaatgtc cgttgaggat ccgaaggcgg gcgcgttcag
     3421 ctactacgcc actcgctact ggtccaagcg cgccggcttc atctccggtt ggaactactg
     3481 gttcaattac atcctcgtct cgatggttga gctgtccgtg gtgggcaaat tcgtaaacta
     3541 ctggttcccg gcgattccga catgggtgtc ggcggccgtg ttcttggtga ttatctgcgc
     3601 cgccaacctg ctgggcgtct ccaagttcgg tgagttcgag ttctggttcg ccattatcaa
     3661 gattgtggcc gtgatcgcca tgatcgtcgg cggcctggcc gtcatcatct tcgctctgcc
     3721 caccgcatcc ggcatcaaag cgagctttgc caactggttc accttggaag ggggcttctt
     3781 cccgaacggt ctcatggagc agactaagga cggcacctgg accggcctgt tgatggcgct
     3841 tgtcgtggtg atgttcagct tcggcggcac cgagctcatc ggcatcaccg ccggcgaaac
     3901 cgaggaccct cgtcgtacga ttccccgcgc caccaatgac attatctggc gaattctcgt
     3961 gttctacatt ggcgcgctcg gcgtaatcat ggccgtaata ccgtggaaca ccatcggcgg
     4021 agacgatgtt cccagcccgt tcgtgcaaat cttcgattcc gtgggcatcc acgcggccgc
     4081 cggcatcctc aacttcgtgt gcctgaccgc tgtgatgagc gtctacaact ccggcctgta
     4141 cgccaactcc cgcatgctgt attcgctggc caaacaaggc aatgcacccg cgtatctggg
     4201 caagctcaac gctaagggcg tgccggtggc cggcgtgctg acgtccgccg tgatcaccgc
     4261 catcgccgtg gtcgtggtgt tcgtgtggcc tgagttcgcg ttcaactatc tgatgtcgat
     4321 tgccaccatc gccggcatca tcaactggac gatgatcatg ttcaccgaaa tgaagttccg
     4381 taaggtggtc gccgcgggcg gcgctcccga ggattccgag ctggccggca agtccggcaa
     4441 ggaagcgctc gacgcgattc acttcaagct gccgttcgcc aaagtaaccc cgtgggtggt
     4501 attggcgttc ctgacactgg tcgtggtgct catgtgcttc tccgccagct accgcgtcgc
     4561 cgttatcgcc ggcgtgatct ggctcgccat tctctttgcg gcctaccagc tcactcaggc
     4621 gaagaagtaa ggctccattt gcgaatagct ctcactgatg agcactcagt gagagctatt
     4681 cgcaaatggg atttattcat gaaacagctc atcgatgccg acgagctgaa cgtcggattg
     4741 agacgccgca gcatcgtcga ccagtgattg atcgaaaccg ccgagcgaaa acagcaggta
     4801 tgttcgctgg ccgataccgg ttcgcgcagc acggtctcgc agtagattca gcacggaacg
     4861 gtccaccggc gcattgcgga atttgcactc gcccacaatg gcagcatcag acgcattgtc
     4921 aacggccatc acgtctatct catcctgacg atcccaccaa cggccaaccg cacgcgcacg
     4981 gaagggcaac cgtccagcag cactctcacg ccacatccat tcacggcaca gaccctcgaa
     5041 cggtttgccg gcaaaatcat gtaataccgg cttgatatga cgctcgtata cgccttccac
     5101 atcgccccgc tccaattccg agcgatacgg aaatacaaac gaataccaga atcggaagaa
     5161 attgtcgttc agctgataca gcccacgcga acctttcgcc cgctccttag gcttcgccgt
     5221 gactgggaac tcacgttcca caatccccag ttccataaga ttggcgagat acgacgatac
     5281 tgttgcgttt gccatcatcg tgtgtgtcgc gatctcattc agcgaagttg cgccgagcgc
     5341 gatcgcccgt atcacgctgt tatatttcgc agtttcccgc agttcttcgt gcaacaaaaa
     5401 ttcaacctcg ctgtatagcg gagcaatgcg tctcagaata tgccgcttga catttgcgtc
     5461 tatggtctca tctgggtcaa attcctctag gtaacgcggg ataccgccca gtattgcgta
     5521 ggccagcgcc ttatcctcgc tactatagtt ggggaagaat tgcgctgcat cccaatatgg
     5581 catcggcagc atcttcagga tgccggtcgc acgaccatat agcggcgatt tttctcccaa
     5641 cagctccttt tcgatgaagc tcatggcact gccgcaaatc acaatcatca gatttgcatg
     5701 acgcaacgtg tggtcccaaa gattctgtag cactgacggc aatgacggat cagatttgac
     5761 cagataagga aactcatcga atatcacaag cctgcggccg ccatcgctcg gctctggcag
     5821 ccgggttaac tcggttaacg ccctttccca agaaggatat tgactcagat aatccttgcc
     5881 cggggcaccc gcctcgaaca tgagacggga gaacgatgcc agctgttcat ctttagtggc
     5941 attctgcgcc gcgaagaata aggtttcctt gtcttttgcg agttgaataa gcgtctctgt
     6001 ttttcccacg cgcctgcggc cgtagacgaa cacaagctgt gccctacgct cagtgaaaca
     6061 ttcctgcagt tgccgtaaat cgctatctcg tccgatgaat gtcatactgc ccgcccctct
     6121 atgccatacg ttggcgaatt cctattcgca ccacgattta ttgtatcacg attaatataa
     6181 tcgtgattat ataagatgcg ggcggtgcgg actcgggccc acttgcaaaa gactctcact
     6241 gaggagtgct cagtgagagt cttttgcaaa atcaggccac tcgttgcaaa tttctctcac
     6301 cgactcaacg tcagtgagag ttttttgcaa atgaggggtc agatacggcg cttgcgccag
     6361 atggtcaggg cgataccggc ggcggcgagg accaccacgg caccagccac gccgaagacg
     6421 gcggcaccag tgtcggagat gacggcaccc ggcttcttgc catcagcgga gaccttcacg
     6481 agggccttgc gggcctcggt caggtgacga atcgcatcct ggacctcggt ggagatggcg
     6541 ttctccttgg cgagcacctt cttcgcttgg gccagcgcat tggcgtaggc cttccacgaa
     6601 tcggcggtgt aatcgtcctg gttcagcttc tcggcttcgg ccttggcaat ggcgtcttcg
     6661 agctgcttga gctcttcctc agtggccaga tcgggatccg gagtcggcgt ctcgtcggca
     6721 acgaccttca cggtgaacgt ggcggtcttg gtcgaatcgg ccacagactg cacggtcacg
     6781 gtcaccttgt cggaggcctt cgaggagtcg aagccgagca gctcgtactc atcggcggcg
     6841 agctcacggg tggtggagcc gccgttgcca tcggccacgg tcgcggtgac gaccagaccg
     6901 gcaacgtcga gcgcttcacc aaccttgtac tcggccttgg cgggctgcgc ggtcacggcg
     6961 atggagagga tcgtgcctgc ggccgggtgc tccggctcgg gctggctctc ctccaacgca
     7021 ttctgcgcgt cggtcagggc cttggcggcg gcatcgacat cagcctgaga caggccggag
     7081 tcaaggaagg tctgcttggc cgtcttggct gcggcgagcg catcctcgaa tgccttccag
     7141 ctggcctcgg tgtaagcaga gccatcggtg ccgagcttgt cggccgcttc aatcgcgcca
     7201 tcaagcgccg tcgtatcggc catctcaatg gacacgcctt cgtcggtcgg gtgaatggtc
     7261 tgcatcaggc cgtccgcgtc gaaggtgatc tcgtcgatgg cggtttcacg gtggtagccg
     7321 aggccggagg tgaacacgcc gagcggcgtg tagtggcgat ggtaggccat gagccaacgg
     7381 tcgttgccgt ccgcgtcggt cacatgcacg tcggactggt gcgcggtacc ttgcaggttc
     7441 ttggaggaat ccttctgcag cagcacaccc ttgtaggtga tggtgccgtc gatggaatcg
     7501 gagacgccgt acctcacatg gtagttcggg gagttggcat cgtcgcatga ccaggtccag
     7561 tggtacttgc cgtcgcggta ggcgacgacc acggactcgc ggaagtcgtt cagaccgttg
     7621 agcttcttca ccgtaccggc cttgatgctc accatgtcgt cgttgagttc ggcgatggcg
     7681 ggcgaaccgt tgccgtacag gatgtacgac ttgccggtgt tcgggtcggt gaagatggac
     7741 gggtcgatgg cctgtccaac ggtgacgcct tccatcgtgc gagtgaccag cggttggtcg
     7801 gcggccttgt acgggccggc cgggttgtcg gccacggcga cgccgatggc ggatgtaccg
     7861 ttcggcaact tcgcgcagta gtagaagtag tacttgccgt tcttcttctc gatggtcggc
     7921 gcccacgcgg agcccacgga ccacggggag atggcggtgt tctcatcgcc atcggtggtc
     7981 ggctggtggt ccttattgac atcgaggatc acgccctcgt cggtccagtt caccaagtcg
     8041 gtggagctga acgcatggaa gtagttgccg ctccagccgg agaagccgtc ggtggtcggg
     8101 tagatccaga acttgccgtc gaagtagtcg atgtccgggt cggcgtactg gccgggcagt
     8161 accgggttgt tgcccacggt gaccttcttc agcgtccagg tctgcggcgc accgtcgccc
     8221 aaatcaacga ccaaagtacg gtccttagcc aggtcggccg taccaccatt ggtgaacgcc
     8281 tgaccatcaa gggtgagctt gatggagtcg tcgctacggt tgacagacac cttgacgggc
     8341 accttggtcg cgccgccacg gcggttgagc acggtggtga cggtcttcgt ggaccagtcg
     8401 agggtggaac ggacctgaga gtggtcgtca gtgccacgca gcgagttcgg gtcggacggc
     8461 acagtgccca cagaaatacc ctgcagagca atcgggctgg tcttctcgac ttcctgagca
     8521 gtgagcgcgc ggttccacac gttcacattg tcgaacgaac ccttgaagta tgggtcgttg
     8581 tagaaggact tgcccaagta ggccagcaga tcgttaccca aatcggtgac cttgttcttg
     8641 gtcgccttgt tctgagccac ctgcgcaccg tcagagtaca cggtcatcac gttgtcgtcc
     8701 aacgtgaccg tgacacgatg ccatgcgccg ttgccagaca acgtagcgtt agccgcgtcc
     8761 tccgcaccat acgagttggt ggtgatgcgg ctggcgagct cgccggagtt gttgtacttc
     8821 aggaagtagt acttggtgga gttctggccg aaagcgaacg tgaactggtt tttattgatc
     8881 tccgacttga catccatctg cacagtcagc ttgttacggc cgtcgaacag gcccttcggg
     8941 aactcggcgt aggtgccgtc agagccgtcg agcttaagca ccttgccctt ttcggcatcc
     9001 tcggagacct tggcgttgcc gttgaaggtc aagtcgttgc tgttctcagt ggtgtcggta
     9061 cccttgctgt cttcgaagtc atactcggca atcggaccgg agccgtcttc atcgggtgtg
     9121 actggctcgg tgtccttgtt cttatcgaat gccttcagga ttgcgttgta ctcggtttca
     9181 gtgacaggca ggatggtgcc gtgacgcttc ttgagcgaac cgaagttcac atcagaggca
     9241 aagccccaat cagccttatc agtgctgcct aaatcggcag aacggtatgg ggtgtaaccc
     9301 ttaccggctg agtactggtc ccacatcaga ccgtagagca tcttcttgcc cgcatcactg
     9361 gtctgcacgt catcttcatt gtagaagaac agctccgggc cttccagctg cgcaccggtc
     9421 agaccggtac cagtcaggtc accgaatgta ccgacaagcg tccactggtt cggatcactg
     9481 tcagtgttgt agtaaccatt cgcaattgtg tcaccagtgg tgagcgttgt ggcatacgga
     9541 tccttgctac gctcaacacc gaggtacgtg tcaccggaca cacgatagta atagccgtca
     9601 tcggccttaa tcatcgtggt atcgataacg gacttgactc gatcgatcca cttgacaggc
     9661 gtcgtgaagt tcacgaaatc acgcgtggtg gagtagtaca tattggtgcg atcgccggac
     9721 ttgttgtccg cgtcggaagc agtggcccag tacaccatgt actgcttgtt cacatcatcc
     9781 cagtaggctt ccggagccca agccatgccg gctcccggaa tctgagaggc aacgtcaacg
     9841 gcgcgcggtt cgctccagtt gacgaagtca tgcgactccc acacgattag gttggtgctg
     9901 ccattggtgg tagcggtggc cgcgccccaa ccgccacggt tatggatgct cagctcggta
     9961 gcgatgaggt agacggtgtc gccttccgga gagcggacca gatacgggtc gcgcacgccg
    10021 ttgtccttac cacgagagtt gccggtctgg ctgttgttcc agctgagtac cggatcgccg
    10081 gactcgcggg tgtcatgcca gctcaggcca tccttgctgg tggcgaagta catctgctcg
    10141 tcggtaacgc taccttcggt gccggtgaag tgcgcaaaca gatagtccgt ggtcttcggc
    10201 tgttcgacgg cggccttgac ggtcagctcg aattccttgg tttccgtgtt gccgtcagca
    10261 tcggtaatgg tggcagtgag cgtcaccttg gtgtcggttt tctgacgagc aacgacacca
    10321 gctgcgatgt caccatctgc cgcatcggta atgacctttg cgttgctgga tttccacgta
    10381 atcgcggaac cgttaaccga gcccttggcc ggaagtgtga tgttggaata cacatcagtg
    10441 ctgccgttgt taatggtcag cgcattagcg tcggccttgg cggcatcgga cttcgtggaa
    10501 tcgtaggctt ccttgatttg gtcagcagta tgcgcagagc cgtaaatctt gaggttgtca
    10561 agtgcgccgg tatagtattc gccattcacc cagttggcct tgccgatctg cagcacgccg
    10621 ccgtctgcgc cgagaatatc ggtgagctga gcgaagctcg cgccatcagc tggggctacg
    10681 gttgcggcct gctcaccgtc aatgtacagc gtggaagcgg cctcgtcgat gaccaaatcg
    10741 acgtgacgcc actggctggc gagaccatcc ttgctcacat tgccggtggt gtcgcgctta
    10801 ccagcgttgt tgtaacgctc aacgttcacc gaagtggtgc gatccatcac gccgaggtaa
    10861 tgctcttgct gataggtctg cgcattcgta ttcggtgccg caaataccga ccagccctga
    10921 ttggtgctgg cggacttgga atcgtacgaa atcgtgaccg catccaggcc cttgagcacg
    10981 gcagaatcgt ccgacttagt cacattcagc cagaatccag aaccgagccg aactgcagca
    11041 gtgccgtcct tgctggtagc cactgcggca gcaccgttga tggtcgcctt ggtgccggtg
    11101 gtgtcatcga caatagtgcc agtagcgcct gcagcgacgt tgtcgaaatc aagactggcg
    11161 agcagatcgc tgtctgccgc tgccttggca tttgatttca gggctgctgc cagcgcccgc
    11221 tgcgcggccg atgaatcgat gggatccgat tcaaccgcat ttgctgctgg cgccagcgcc
    11281 atcagcatcg ggacagctgc aagagctgcc accgctttct tccacttacc catatgtctc
    11341 ctctgattgt gggttttggt tttgtgttca gttatggatt gaagattgaa gttatggtga
    11401 tactaggtgc tccctctccg tccgccagaa gccgactgag agggagcatt tggatccgct
    11461 caggcgcgac gcttgcgcca gatagtgaga gagaagccgg caacggcgag cacaaccacg
    11521 gcaccaccaa ggccgagcac ggcggcacca gaggacggca gaccggagtc tgctcccgta
    11581 ccagcgttac cgttatcgct gcctgcacca tcgccggaac cgtcgcccga accgggctta
    11641 ccgggctcgg tcttggcact cacggtcacc acgaacgacg tggtgagctg atcgttggcc
    11701 ttgagcgtga cggtgacggt cttggtgccc acagcggaca tgtccacgtc gccgaccgtg
    11761 tagtcggcgg catcgagcgt cttcaccgtg ccgtccgaca tcgtcgcctc gatcacaaga
    11821 ccatcggcgg agaactgctc gcccaccgtg tacttggtct ttgtcggctc gctcttgacg
    11881 cggatgccgg tgatggacgg ctcaaccggc gtatcgggct cgccgacggt caccgtaatg
    11941 gtcaccgtgc ggttggccgg agcaacctcc tgatccttgg tcagcgacat gtcgagcatg
    12001 ccgttggcgc gcacgttcac ggccttggcc cacgtattgc ggtcctgatc gaacaggcca
    12061 cccgctgcgg aaccggagtg aatttccggg taggggcgtg cgtggtaggc catgacgatg
    12121 ttgccggcct catcatgtgc aagcgagatg tggccggggc ctgcctgctt gcgctcgtac
    12181 gaggcctcat ccgcaccgta ggcaccggtg taggtgtcgt tggtgtcgaa cgacgggaag
    12241 tcggccgtgg tccagctcga cagatcctgc aggtcggcat tcttggcggc acgcatcacg
    12301 ttggtgttgt aatacttatc caccgtggca cccgcatagg caatgtagac gtagtcgcca
    12361 tgttcgaaca tgaacggtgc ctctacgatt gcctgatcag aaccttcaga gttgtctccg
    12421 ggggtaggcg catacttgcc gtactcgaac tgctggctgg cctgatagat gtgctcaagg
    12481 ctgccatcca ccagcggagt gacgccatcg cccatctttg ccttggcaag gtacaggcct
    12541 gcgctcttcg ggaagagcca gtagccctgg gccttgccgt tctcctcgcg ctcgaagtac
    12601 gtcacgtcga atgcggcgcc ttccagcacg gtcggctcgc cccagttttc ggcctcgagg
    12661 aatccgccat ccttgatgga ccgctcgtcg ccggtgtacg ggatgaggat ggtgttgttg
    12721 caccagccac cgttcgtgga cgaggcaccc ctgtggaagc cggccacaat ccaccacttg
    12781 ccgttaatct tgtggaactc ctgggcccaa atgaacgcgc tccaaccgta gaacgagttg
    12841 gggtactggt cttcgtggcc cggagtgccg ttctccggct tgatgatgat gtgttcctca
    12901 gcatccttca gaccgttgat ggtcttggcg cgcttgaggc cgatggaacg gtagctgccc
    12961 ttctggttgt tggtcgaatc agtggacttt acctcatagg tggaaccggt gaggtagtag
    13021 tagccatcgt cttcgttgta gaagatgtcc ggatcggcgc gagcgtcgtt gtacatggcc
    13081 tcggtggtct gctgaacagt gcccttgacc tcatacgtgc cggcctttga ggtgtccacg
    13141 gcggccagat catcggcgtt ccagtcgacg ttgaaggtct tcgtggagcc gtcgttgtag
    13201 gtcattgtgg cctgcgttcc ggaaaccgct gcggtgacgt cctcggcgga agcaccgttc
    13261 tccacggttg cgccgcccag agctttaacg ccggtgttct gcagatcgac atagttcttg
    13321 tagaacgcat cgaattcaga ggagctcaca gcgaatgcgc gggtctggcc ttcgccggcc
    13381 catgcgggca gaccttcggt cacgtcgccc acacccatcg caaccgcgtc ggccttgagc
    13441 gtctcgccga ccgttgcctt ggcagtcagg tcggagagcg tggccatacg gccttcgccg
    13501 ctcaggccat cggtccagaa gaccttgtag cccttggagg cggcatcata gacgatgcgc
    13561 gggttggtga ccatggagct gtcggtcacg gtgatggcgc gctcgttggt gaacgtggca
    13621 ccgtcgccct tgccatccca cacgtaaatc gcaccggtgg cgttgttctg cgaggaaacc
    13681 acaccaaggg taccgtcctc cgcacggaag aaagcgggcg agcccatctg cgcattcggc
    13741 ttggcagact gcgagccgtc ccacttgacc gacagaatcg actgcgaacg gttcagggtc
    13801 tcccatttgc tgccatcggc ggccttggcg gccacgtaca gcgagtcggc gcgacgatcg
    13861 tcgttgtagg cgagcgggtc gtacttggca ccgttctggt cggagcgatc gacatccttg
    13921 acgtaggaag cgatttcgcc gccgttgttg aggatttcgg attcggcggt gatggcctta
    13981 tccacgccgt cgatggtggc ggtgagcttg accttcacgg ccttctcgcc ggacttcggc
    14041 tgcgtgacct tgccgtctgc ggctacgagc ttcgcaccgt cgccggtcgc ggaccaagta
    14101 accgtcttgc ccagcacagt gctcggcagg tcgccggtga tttgcgccgg gatggcgagc
    14161 gcggcggcgt agtcggtctt cgcgctttcc acgccaagct cgtcgtcgct cagggccttg
    14221 ccccagatct tgaagttgtc gagcagaccg gagaagtact cgccgttgct ccagttggcc
    14281 ttgccgatct gcagcacgcc gccatcagca ccgaggatgt cggcgagctt ctggccgttg
    14341 ttgttcacgg ctaccagctt ggagtcgatg tacagcttgg cggttgcgcc ggaaatcacg
    14401 atatcgacgt gcttccagtc ggcgttcgtc ttggtgtatg agacgttgcc ggaggaatcg
    14461 cgcttgccgt cgttgttgtt gtagcgttcg acggtaatac cggtggtctt gtcgagcacg
    14521 ccgacgtagt gttccttgcc gtactcctgc ttggtggcgt tggaggcggc gaagaaggtc
    14581 cagccagtgt tgttggaggc atcgggcttg acgtcgtagc tgatggtcac gtcgttcttg
    14641 cccttgagcn nnnnnnnnng agcagagcgt cgccgttctt ggcggtgacg ttgagccaga
    14701 agttcttgct gagcttcgcg gccttgccgt ccttgccgtc ggcgagcgtg gcggtgccct
    14761 gcgtggtgcc cgtcgcgttg ccgccgtcaa aggtcttatc gccatcatcg aacgtgaagt
    14821 cggcaagcag atccgggtcg gtcggctcgg tttcgccggt cacggtcacc ttagcggtca
    14881 cggtgatggc ggtggagctg tatagcgtgc ggttcttgtt ggcggtttca ttgtccacaa
    14941 agccaccggt ctgcttccag ctgttgttcg cgttcgcacc ggagttgatg annnnnnnnn
    15001 ngtcttcgac tcgccggcgg cgttcgtcca cttccaatgg ttctcgttgg caccgatggt
    15061 gtcgaccgtg ccggtgacgg tgtattcgcc gggcttggag gtgtcgacgt tggaaacgtc
    15121 ccacgtcacg tcgcgcttgg cagtgccgta accgttggca agagtgacct gctgcgtctt
    15181 cggcagcttg gcagtgatgt ccgtatcggc agcaccctta tccaccgtca catcctcggc
    15241 cttcaggtcg gcgttgtcgg aagccttggc gtcagccgcg cggatcgcgt cgtactgggc
    15301 cttggtgagc gagaccacgc caccgtgctt ggtgttggcg gtgaggaagt aatcgtcggc
    15361 cttcaggacg ctccacggat tctcggaatc gaggttgtcg gcttcgagta ggttgtagcc
    15421 gtagcgggca ccggaggcga cagatccata gttgtcgacg aacatgtacc acttgttttc
    15481 gccgttgacc ttgaaaccgt tggcaccttc ctgcgcgctg ccgagcgtgt gtccgatgtt
    15541 ggtctggcgc aacacccagc ccttggccgg gtcggtcgcg tactggccct ggtcgttggc
    15601 acgccaccaa tcggcacgat cggtgtattc catgaagatc acgccggaac cggtggtgcg
    15661 gtatgtgcgg gaaccgttct gcagcatcgt ggtgtcgatg gtgttgccac ggttgtactt
    15721 gctgttaggc acgaagacgc cgccaaacat gtaggtctca ttggtgaagt ccttggtggc
    15781 ggcccacagc acgtgagtca ccttcttgcc gggctgactg ttctcgccat cgtatttggc
    15841 ggagacagac caatacacaa cgaatgcgcc gccctgctgg ttcttcgggt tgtccttatc
    15901 ggaaccgttg agctgaccgt cgtcgttgag atcatagaag ttcggcaccc acgtagcctc
    15961 aggagcccat gccatgccga tctcggagat ggtcttgccg gtcacggaat cggtgagcgt
    16021 ctggctcttg ttcggattgg cagtgtactt gtcggcggtg gcggtctcca gttccgtcat
    16081 gtcgttgaag tgaatgagat ctttggacga ccacacatgc aacttggtgc tgtaacctgt
    16141 agcccacgtg ccccaaccgg cattatcgct gccgaacaca cgcaaatcgg tggccagcac
    16201 gtagtaggtc ttcgactcgg ggttgtaggc gatgaacgga tcgcgcacgc cggtggtgga
    16261 ttcgttgctc acaagaatcg gctcgccgcc gttgagcgtg tcccactggg tggcgtcatc
    16321 accgcgtgag atgtcgaggt agaccttttc ggcgtagccc ttgttgtttt cgatgaagtg
    16381 gaccatcacg tagccgtagg cgtcatcggc cttgacctcg gtgacggtga ccttgagctc
    16441 ggcggtcttg gcggcgtcag cttcggaagc cgctgtaatg gtggcggtgc cggccttcac
    16501 gccggtgacc ttgccgttgg catccacagt agccacagca ggattgttgg attcccacgt
    16561 cactgtggga tcgacacctt caggggtaac ggttgcggac agggatgcgg agttgccttc
    16621 cttgagactc agctcgccgt ccttcacatt ggaaccggaa atctcgattg cagtaggcga
    16681 tgcgatgccg agatcggctg cggaaagcgc ggagccataa atcatgaaat tatcaagcaa
    16741 accggtgaag tattcaccgt tgccccagtt gcctttgccg atttgcagca caccacctga
    16801 gccgccaaga atactcttaa gatcctcgcc gttcacgttg ctcgccacga acttcttatc
    16861 gacatagagc ttcgcggtct tgccggagat gacgagatcg acatgcttcc aagcatcctt
    16921 cgtaccggtg ttcttgtcga tggtggcgat gtcggatgcg cggccgttga gatagcgttc
    16981 cacacgggtc ttgtcggtgc ggtcgagcac accgacgtag gtgggggcgc tgccgttcac
    17041 cgcaccggcg gtcggcgcgg caaacacagt ccactggcca ccggtcgcag ccgccttgct
    17101 gtcgtagctg atcgtcacat catcaagacc gttgagcagt gcactgccat cggacttggt
    17161 cacgttcagc cagaaaccgc tgccgagctg ggcggccttg ccgttgtcgt catcttttcc
    17221 gttgacgaga tccaccgcgc cttgcacggt ggccttagcg tcaccctgag cactggcgaa
    17281 tgcaccatcg tccgggtcgc tattgaaatc gaagctggca atcgggtcag ccgtcgctgt
    17341 attggccgcc gccctgacgt tcgcactggt cgtggtcatc tgcaccgcag gcgtcgcgtc
    17401 gtcggccacg gcggtgatcg gcatgatcaa ccccgcgccc aacgtcgaca ccgccgtcag
    17461 gcaggccgcc aatcgtgtaa ctctcctcac tgagtttcct tccttgggtc cttgcttctt
    17521 tgcaatactg tgatggatca tcgttgcgcc atcgcaatat ttgttatcga attgttatca
    17581 agtgacaccc ggtcgctcct ttatcgaccg cttctcgctc ctgcttccgc ttcgccggag
    17641 cctcttcgtt cgccagcgcg acccacttcc gcccgaacga cggccaccgg aaatccctac
    17701 gaacgacacc gttcgtgaac aggagcaccg atgtgcaatc gcacaggttt ctgtaagcac
    17761 tcacaattct aatgtgagcg ttcacctaaa tttgagcacg ctttgagcga cgacgcgaaa
    17821 gcggtaacgc acatgctgat tccttgccat acctgaaata cccggcaccc ctcttaaaac
    17881 cggtttcatc tccccgaatg aacatttacc ccttctcagc accccacaac aaacaaaatc
    17941 gcacatcatc tgtgtgcaat attggcttgt tgagccgcgt ggcccgcatg tccgcgttat
    18001 ccagttcacg ggatcacgct ggtaaatccc gtgaactgaa gacatgtgca atcgcacacg
    18061 caaaagattg gaatcaagcc gaactcgatg gccggccatg tgcatttctg taagtttctg
    18121 gttcacggga ttttcagccg aaatcccgtg aaccagaaac ggctccgtac cgagcgtatt
    18181 tccgagttgt agggcacgcg cccgggtact cgccactccc agagccgatc aaccacccga
    18241 aaatggcgga ataccaacgg gctctcatcg gccgcaacgg ccaatacttc agccattgcc
    18301 ctacaactcg gaaatacacc tccacacggg ccgatttcgc gattggaggc ttacgccgct
    18361 caggcctggc gcttgcgctg gagggtcagg gcgatgccgg cggcagcgag cgccacggcc
    18421 acgccgctga ggccgagtac ggcggcaccg gtcttagaca gaccgagctt gttgcccgtc
    18481 ttatcaccag tagcaccagc gtcaatgctc gagccgtcgc ccggcttagt cgaatcgctc
    18541 gagccatcgc ccgaatcgga accgtcgacc ttcaccaacg cggcctgcgc cttgttcaac
    18601 gcggcggttg cgtcgtcgac gtccttctgc gaagcgttct cgtcggcata aaccttctcg
    18661 gcggaggcca acgctgcggc gaacgccttc cacgaggcgg cggtgtagtc ggactcgcgc
    18721 agctgcttgg cggacgcgag cgccttggac aagtcggtct tgtcgaccgt aaccgtgtcg
    18781 tcgaccacct tcactgtcac ggaaacggtc ttggaggcca gctcctcgga agagctcatg
    18841 tcgaacaccg gcataccgtc ggcagcccag tgcacgcggc gtacgaacat gtcgcggccg
    18901 gtggcattca cggagccgag gttcttcgta gcataagcat ggaacacgta gatctggttg
    18961 ccgtcctcgt cctcgctcca catgccgtga ccggtgccga gctgcatctc gccattgaat
    19021 ggaccggact tctgaatcgg gtagttgagc gccgtccagc tggccgggtc ggtcaggtcg
    19081 gagccggcgg catcagccac ggccaaaccg gtggtgtagg tcttgcctac gccggaaccg
    19141 gagaacatga ggtacagctt gccatcacgc atggtcacgt tcggaccttc ggctatggcg
    19201 gcgttccacg catactccgg ggtgacgatg cgcaccgggg agctggtcac atgggccggg
    19261 tccttcggat ccatcgtggc gatgtaggtg gcgccgagct gctgccaggc gtagtaggac
    19321 ctgccgtcgg catcctggaa ataggtcatg tcgagcgaca tgcccactcc gccggacgcg
    19381 gtggctgcga gctgcttgtt accgttgagc agcgaggcgt cattgcggta gatcggtgtc
    19441 ggcacagtcc agttatcggg gtcggtcggg tcgaggtcat gaccgtcggc atcctgcttg
    19501 agctgcatgt acaccgactt gccgttggac catgtgttgc cgtatccggc catgaacaga
    19561 atcgtgagcc taccatcaat ctcatgaatt tctggagccc agaagctgtg catgatggca
    19621 ttaccctcgg agtccttgtc gccagccttg agcagcgaca cctccttgtt gttgtagccg
    19681 ttcgactgaa tcagggcatt cgggttgccc ggctcgtcgg cgagaccgga aatcgtgtcg
    19741 gccatgcgga acggcatgtg gggcgaacca tgctgccaag tgacatcacc ttggatgtcg
    19801 ttggaagcga tcatcaggaa cttggtttcg gtgacttcct tgccgtcgac ctcatgcgtc
    19861 cactgccact tatacaccga tggatcggcg cggtcctcgg cgaacggaat ctggtattcg
    19921 gtctgcttga cggtgccggt gacagtgtaa tcacccacct tcgtcagatc tataccctca
    19981 gtgttccatg aggaaatcgg cagggagccg gtagaaccgt cggagtaagt gaggtccacg
    20041 ttcttcggca gttgcgcggt cacctcatcg attgaggcgc ccttcttgac ggtgatgtca
    20101 gtgagattgc tcgtgccggt gttctcagag cggccgtaac gggtgttgag cgccttcacc
    20161 gtggcctcgt ccaccgcgat ggtggcaccg gagcggaagt tcgcgatgcc ctgtacaccg
    20221 tcggcggcga gtacgccgga cttggcgacg gaaccgtaca gtacgctgcc gtgcttggat
    20281 gtggagccct taagcgaatc gaacgtggtg tacttggcca cgccgttgtc atcggcccag
    20341 ccgacaacat actgcttgct ggcggtgtcg tacacggcgt acggagcgtt gacgccgttg
    20401 gtctcgccaa ggtcgacgat ggaaccggag ttctcctgtt cgctataggt gagcaggtct
    20461 tcggaggtgg cgatgagcac gctggacttc gcggtggagt caccggtggc ggtgccgcgg
    20521 ttggtacgca cggaaatcac gccataggtg ccgtcggcca tgcggaacag gctcggatcc
    20581 ttgagactgc gatagtcatt gccgtcgagg ttctcgttga gcttctgcgc cttcggcgag
    20641 cgtgcgaaga aaatgccgta attctcgttg tacggggtgt acgagccgtc ggcgttctgc
    20701 agcgcgaggt gcatgctgta ggcgacgtcg ccgttgttgg cctcattctc gccggtggcg
    20761 ttgcgatcgt aggcggcgat ggtcgcggac ttcgcggcgg gcagcacctt tacggtgaag
    20821 gccttggtaa tgggctcgat gccggcgaga ccgttcttgg agaactcgac ggtgatggta
    20881 ccggtcgtgg cctcgtcgcc atcgatggtg atcttgccat cctcgacggc tacgtcctta
    20941 gtggacgtaa ccgtggcggt agtgccattc acagccgccg ggagggcgtc tccggaacgc
    21001 acgacggacg ggatcacgta gccggccgcg acttccttga gctgttcagc ggcagttttc
    21061 gtggtcgggt tgaccgtgac cgtgaattcc ttcgtagcgg tctggccacg gaccgtgacg
    21121 gcggcggtca gcgtgacagt cttggctgcc tcgcccttgg cgggctggtt gaccgtgccg
    21181 tcagtcgcga tgacgctgct atcggaggac gcccacgtca cgccgttcgc agtgggcagc
    21241 gaaatgaacg gatcgtcgac cgtcgtgggg acgatgatgc cgttcagcac ggaaccggtg
    21301 agttcaccgg catgaattga ggcatcggca gtgctgatcg cggcgacctg atcggcggtg
    21361 agcgcggcat cgtatacgcg gaagttcgag accgcgccct tgaacaggtt gtcgggccac
    21421 ggcgaacggc cgatggtgtt gagcgactgg tccttgacgc tggacagttt ggccggcacg
    21481 gtgccggatg ccacctgcgt gccgtcgatg tacagtttcg cggcggtgcc atcaatggtg
    21541 gcggtcaccg acaaccattg gtcggccttg gccacgcagg agctggactg cacgagcgtc
    21601 tccgtaccgc ccgatttcag gccgacgagc gggttacgcg aactgccgca gttgagcgtg
    21661 gcgaagaaat actccgtatc ggagctgtcg ttaccgatgt tccacaggaa atggaaagca
    21721 ttgagcatgc tggaatccgc tttgacctcc aactgcaccg tggcggcgtc cttgccggac
    21781 agcagattcg agggcagctt gacccagtcg ccggtgccgg tcttggcacc gccggaaagg
    21841 gtgagggcat catcggccca aagcgagtcg gcggagttct gtacttcggc cgcgccaagc
    21901 gtagcgttcg gcgcactgtt ggccacggtc ttgccgtcac ttggcttcgt ggtgaagtcg
    21961 taggcggcga tgagattatt ggtcggaacg gtatcggccg cgaccgccgt ggtcgcgccg
    22021 attcccatgc cggcgagcgc catcggcact gccagcatcg cgccgagcag gcgtttgcct
    22081 atcgatggtt tggttgtcat tggtttcctc cttgcgcgcg gtgatactgc ttgtcgcgca
    22141 gcagatctag atgttgagtg gtgaagttcg gtaatggcta ttggagctat ggggctatag
    22201 agcctatgcg tccgacagcg tcgtcggacg aacgtgcgat acgcccctct gatggggccg
    22261 cgccatgaag caaacaatcc agtaggctgc ttgcttcaca gctcgactca tccagaaggg
    22321 gcttggtcta cgctgcaaac agttcaggcg cggcgcttgc ggaaggcgta ggcggcgccg
    22381 gcggcgacga gcagtgtgac cgcggtgata cccacaccga ataccgaggc gccggtgttg
    22441 gacagagcgc tgcccggctt ggcggcgggc ttttgcgtgc cgtcatcctt gccatcggtc
    22501 ttgccggaat cgccggactt cttgaccagc ttgtcgacgg cggactggag ccgagacagc
    22561 gccttgtcca cttcctgctg gtcggtggag ttctgcaaca cctgctgcgc gtcggcgagc
    22621 gcgacggcga aagcatccca accagactgg taatcagcct tgttatagcc cttcacctta
    22681 tcgacaagcg cctgcaactt ggacttgtcc agcgtggagc cgggctcggc ggccttgatg
    22741 gtgaacgttg ccgagacagt accggagtac ttgccagcgc cggttacggt cactgtggca
    22801 gtgccaggtt cgacgttgtt cgtataggcc accgtgtagt cggcgccttc cttgagcgtc
    22861 gcgccatcga gtacaacggt cacggctggt tccttggcag cgccgtctgc ggt
//
LOCUS       DAWBMW010000012        22710 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_618_length_22710_cov_3.642507, whole genome shotgun sequence.
ACCESSION   DAWBMW010000012 DAWBMW010000000
VERSION     DAWBMW010000012.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 22710)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 22710)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..22710
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_618_length_22710_cov_3.642507"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    255..264
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    12929..12938
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    14689..14698
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    19316..19325
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    20381..20390
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4873 a         6501 c         6648 g         4638 t
ORIGIN      
        1 aagtgccgtc aatcagtgag ctgcggcaac agctggccgc caagcaaacg gaactgcaaa
       61 cgcagcgaga cagtctggcc cagcagaaag ccgatttgca gcgcacatta aacgaaacca
      121 ttgcccccgc acaatccacc ttggaccagc agaacgcgca actgaccgcc aaggaacagg
      181 aagcggctgc aggagaggca cagctcaata ccaaatccgc ggaactcgaa gcgaatgccg
      241 caaccctcga aaccnnnnnn nnnncccagt cggcgcaact tgtagcgcag gccgcccaac
      301 tggccagcgg caagcagcag ctcgaagaag gcgagcggca gctggaagaa ggcgaacaac
      361 agctcgcgga cggcaaggcg aaactggatg atgcgcagtc tgaactcgat gcgatgcgaa
      421 gcgaagcgga aagcgaattc gccaagcagc aacggcgcat cgacgatgtg gccaacgcac
      481 gttggtatgt gcagacacgc gcttcgatcg acggattcag ctcgctcaaa tcagacgtca
      541 gttccatcga atccatcggc cgcgcattcc cgattgtgtt cctgctggtc gccgtgctca
      601 tgagcctgac caccatgacc cgcatggtcg aggaagaccg cggacttatc ggcacctacc
      661 ttggactcgg ctatggcgga ctcgccgtgt ccagccggta tctgctgttc gcgttgctcg
      721 cctgcttggt cggcggcggc atcggtctgc tggtcggatt ccttggcatc ccggcattcc
      781 tgctggtggt gattgaggga ctgtatatat tgcccggtgt gcgactcgaa tacgactggt
      841 tgtacggttc ggccggcatc gtgctgttcg tggtcggcgt gggcgtggcc accgcgctcg
      901 cctgtcgcga ggagatacgg cacacgcccg ccgcgttgat gcggccgaag gcaccgaaag
      961 cgggcgctcg catattgctg gagcggattc gcccggtatg gagccggctg aacttcctcg
     1021 gcaaagtcac cgcgcgcaat atcttccgat tcaagtcgcg cttgattatg accgtgggtg
     1081 gcgtggccgg ttgcacagcg ttgattatct gcggatttgc gattaatgac accgtcgaca
     1141 cgattggcgt caaacaatac gagcagatct accagtacga cctgatggtc gtggccaatg
     1201 acgatgacgc caccgcgatg cgcaagcagg tggcacaaga tggccaaacc accgaaacgc
     1261 tgaatctgcg cgtggatagt ggcgagatga gcaatgcggc gcaggaaagc gaaaccgtgc
     1321 agctgatgac cgtgcccaac gattcgctga atatcttgaa cgatatggtc acgctggaac
     1381 aagctggcga tgatggatgg tttgggctgc ccaatatctt cggcaaagcg ggtggcggca
     1441 ctgtagcgct ggacgatagt ggcgtgattg tatcgcagtc cgccgccaac tcgctgaata
     1501 ttcatgccgg cgacaccgtc acgctgggca atggcggtag cagccgggcc aaggtgcagg
     1561 tcacggccgt gacgcgcaat cttattggct ctgatgtgta catcagtgag cgactgtacg
     1621 accagaagtt tgcggccggc ggtgtgacgt cttcggcttc gggagatggc actgcctcga
     1681 ccttgacgtg gaacgcgatg ctcgccaagc tgaaaggttc cgagaccgaa caggccgaat
     1741 atgcggatcg gttgggcgag gaatcgtcgg tgctcaaggc cgtgagctgt gcgcatctgg
     1801 ccgccacgtt taagttcgat ctgatgggtg ccgtggtggc gctgattgtg gggctcgccg
     1861 gcggtctggc gctggtggtg ctgtttacgt tggccaatac gaacgtgtcc gaacgcatcc
     1921 gcgagatggc cacactgaag gtgctgggct tctatgaccg cgaggtgcac aactacgtga
     1981 accgcgagat gatgatattg accggtatgg gtgtggtggt tggtctgccg cttggcaggt
     2041 ggataggcgg attactgact gctgcgttga atatgccgtc gctgtatttc gaagtggaag
     2101 tgcactggta cagctatgcg attgccgtgg tggcgacgct ggcgttcgcg ctgctggtgc
     2161 agctgttcac caacccagtg ctcgatcgcg tagacccggt cagctcgctc aagtccgtgg
     2221 aatagaggta gaagttcaat ttggtgttga tgaattgaac tttttggcgg aatatttaca
     2281 gtatcatcgc atgtattctt gagctgagcg gttttcgtga catatttcgg tgatacagtt
     2341 tgaacttgtt ttgaacgagt atcgcgtctg gtgcgtaaca atcgggcggc agagaagaga
     2401 aatgaaatca gtatggccga tacgaatgtg aatggtggta cacagcaggc tacgggcaaa
     2461 ctcaagtctg tcgaaagcct ggaccagacc aacgagatgg agcgtggcct gagcaatcgc
     2521 cacgtacagt tcatcgccat tggcggcacc atcggcaccg gcctgttcct gggctcgggc
     2581 aagtcaatct cgctaaccgg cccctcgatc atcttcgtgt acattctggt cggtgtcatc
     2641 atgttcttcc tgatgcgcgc catcggcgaa atgatgtacc gtgaccccag ccagcacacc
     2701 ttcatcaatt tcatcacccg ttaccttggc aacgggtggg ggcatttcgc cggctggacg
     2761 tattgggccg cgctcgtgct gctgggcatg accgagatca cggccgtctc cacgtacttc
     2821 atcaccttct tcgacacgtt cggcatcgac ctgacccatt ggaaatggct gatcgagctg
     2881 tgtttcctgg tgtcgctggt ctccatcaac ctgattgccg tcaaggtgtt cggcgaggcg
     2941 gaattctggt tctccatgat caagatcacg ctgatttgcg cgatgatcgc cacagccgtg
     3001 gtcatgatcg tgatcggcta ccattaccca gccgtgcaga ttcacggcgt ggaccacgtg
     3061 agcccggccg gccacgccgg attcgacaac ctgttcgcca acttctcgtt cgcgccgaac
     3121 ggttggatgg ccttcctcat gagcttccag atggtgttct tcgcctacga gatgattgaa
     3181 ttcgtgggcg tcaccgtctc cgagaccaag aatccgcgca aggtgctgcc caaggccatc
     3241 aacgagatta tcgtgcgcgt gctcatcttc tatgtgggcg cactggtggc catcatgtgc
     3301 attgtgccgt ggacctcgtt caagccgaac aaggacggct ccttcgcatc cccgttcatt
     3361 atgatgttcc agtatgccgg gctgaattgg gcgtctgcgc tggtgttctt cgtggtgatt
     3421 accgccgcat cttccgcgct gaactcgctg ctgtactcgg ctggccgcca cctgtaccag
     3481 ctgtccgagg tgtcgccgaa cccgacgctc aacaagctcg gtcaggtgtc cgatcgcaag
     3541 gtgccggccc gcgccatcct ggtgtccgcg gcgctgatac tgctgtcgcc gatcgtcaac
     3601 gccatccccg gcgtgtccgg agcgttcgtg ctgttctcgt ccgccagctc cgcggtgttc
     3661 ctgttcatct acatcctgac gctgatggca caccgcaagt accgtcgctc cgctgatttc
     3721 attccggacg ggttcgtcat gcccgcttgg aaggtgctca atacggtggc aatcgtgttc
     3781 ttcgtgttca tctacctgac gttgttcctt gcggacgata cgcgcaattc cgcgattgcc
     3841 ggcctggtgt ggcttgtggg cttcggtgga ttctctatgc tgcgcgaacg ctaccgtagc
     3901 cgcgatttga aggccgcact ggaacacaag gagtgattgg acgggctgat tggctggcgg
     3961 ttattagttg ggtggctgtt ggcggctggc ggctgactgg ctagcggccg atagccaatg
     4021 actgggctga tcgtcagcca atcggctgcc tataggtctc attgatacat tcggtcatcc
     4081 gattagtgca tttgcgcgga caattagtgc attcgatcat cggattagta catcagtgtg
     4141 caattattag tacaattagc cgcctaatta gtacattagg acataaaaca atgtcctaat
     4201 gtactaatta gatgctaaaa tacgataagt gtcatgtcct aatgtatgaa ttatcgctat
     4261 gaagggcgga atgcgatgac gcaggatgaa cttatgcaag tgctgcaggc cggtgaatca
     4321 tctatggtcg agttcaagcg ctgcagcgaa ttgcctcata cggatacttt tgagactgta
     4381 tgctcattcg ccaatcgttc tggtggcagc atttatcttg gcgttgacga tgacggtcaa
     4441 gtgctcggcg tcgattctaa gcgcgttagt gagatagagc gcaacgttgt caacgtagtg
     4501 tctaatcgta agatgttcga accggctcca atggtggaaa cggaacatat cgagtacgac
     4561 gaacggatta ttgttcgtat ttgggtagct ccgagttctt cggttgtgcg gttcaagggc
     4621 gcagtgtttg atcgcattgc tgatgtggat acacacatcg aaagcgatat ccagattagt
     4681 caaatgtata ttcgcaagca gaactactat tccgaacggc gaattttccg ttacgtgact
     4741 ccgtttgatt tgcgtcctga gctgattgcg cgaatgcggc agctggcggt cgctcagcga
     4801 gctgggcacc cttggggaac tatgtctgac gaagaactgt tccgcagcgc caagctatac
     4861 gatcgtgatt acacgaccgg cgaagagggc tttaatcttg ctgcagtgct gctcttgggc
     4921 aaggatgaag tcatttcctc ggtttgccca gcgtatatta ctgatgccgt ggttcggcga
     4981 gacaatattg accgatacga tgaccgactt atggtgcgga ccaacttgtt cgattcgtac
     5041 gagcaactcc gtgaatttac tgagcggaat ctgcctgacc gctttgtgtt aaagggcgat
     5101 aagcgagtga gcccgcgtac gatgatcgcc cgtgaattgg tggcaaacag cctcatgcat
     5161 cgggagtatt ccagtccggt ggtggcgcgt gtcaccattg ataatgaatc catccggacg
     5221 gttaatgctt cgcgctcatt ctttgaaggt cgaatgaagc tgggcgagtt cacgccaatg
     5281 ccaaagaatc cgattatcgc taatgtgttt gtgcagaccg gcatagccga gcagccgggt
     5341 agcggactgc ggaatctgat aagagcgtcc cggcaatata ctgagcgaga gcctgagttc
     5401 cgcgatggtg acatattcga ggcgacgatt cctattgttc ctgcgctgaa gaccgtggat
     5461 ggttctggtt ttgctagcgt gagatctgta gttgagaaag atgccgaaga gggtgcgagg
     5521 ctcattggcg gggaacgtgt ggatggtaag aaagcagatg caaccgcatc gggtgaaatt
     5581 gattcgcggg cagcgatgct gtcggcgatg aatcgacgct tgacagaggt tgactatgtg
     5641 actgtgcctg aactcgccaa gataacagag ctcgataaca gaacagtccg caaattccta
     5701 aacggtttag ttgcgcaagg aaagctgatt gctgaaggca acacgcgtgg tcggcgttat
     5761 cggaagggat agcgggcgcg atatctcgat gcagtgggat tcacttaagg gctacggcaa
     5821 atatgattcg tagcgattgg gccagcgcaa ttcgaatggc tcatgacttt gtgcgtcgtt
     5881 tgtggtctct gaagttgttt gctgggtgac ggttttgtga taaaatcgct catatcaaaa
     5941 tgtttgcgta aacattttgg ctgcgccgaa agaaggagtc gacgtaggtc atgtatcagg
     6001 caaagaggcc ggctcagtga agcaaaggcc gatttttccg gtataaggcg gacagcagga
     6061 acactaagga gaccaatgaa ggtactgagc aaatcgcttg ctgcaatggt tgcggcggca
     6121 acactagtgg gaggaggggc gtttgcggtt gccggcactg cgtatgcggc tgataacgat
     6181 gccattaccg tgaccccgaa cccgtggtat gccaacagtt tcgatggctg gggcacctca
     6241 ctggcttggt tcgccaacgc caccggcagc ctcggcgagc aatcggccat caccaccaat
     6301 ctcggcgatg acgcttccaa ggctaaggct gtggaatacg gcaaacagct gcgcgaacag
     6361 ttctaccagt ccatcttcgg tgatgaagga ctggacctga acatggcccg ctacaacgtg
     6421 ggcggcggca atgcctccga tgttgcctac ggctacccat tcatgcgcca aggcgctgcc
     6481 gtgcctggca cgtggaaaga tgacgccacc ggctccggca cgtatggcaa tggcgtaacc
     6541 accaagcagg ccgacaaaga caagctggct gcggcattcg acccgactga cgacaaccag
     6601 tatgacttct ccaagtccgc cgcccaagac tggtggattg agcgcggtgc caccggcgat
     6661 aaccctgaca tcaccgacgt agaggccttc gccaactccg ctccgtggtt cctgaccaac
     6721 agcggttacg ccactggtgg acgcaactcc ggtagcaata atcttgcaaa ccctgagaaa
     6781 ttcgctcagt acatggccaa gaacgtcgag cacctcgaaa gccttggcgc aaacgttgac
     6841 acggtcgagc cgttcaacga gtccgagacc agttactggg gcactccggg cgacatggct
     6901 tcgaagtaca ccgatgagag tgatgacaac accaagctca ttaacaacta ctgggataag
     6961 tactactccg acaaagatag gtccgtcacc ccatactcca acgcgctgaa gaagccgcag
     7021 gagggtatgc atgtcagcaa cgcccagcag cagcagacga ttaccgcact cgctgaggcg
     7081 ctcaaggaca atgatgacac catcatcgca gccaccgatg ccacgaactc cgccgacttc
     7141 gtcaagtcgt acaaccagta cccgcaggcg atcaaggacc ttatcggcca gtacaacgtt
     7201 cacgcctact ccgacagcaa ccagatgcag tcgcgcgata tcgctcaggc agacggcaag
     7261 aagctgtcga tgagcgaggt ggacggctcc tggcagtctg gctcctacaa cccgtacggt
     7321 ttcgacaacg cgctgggcat gatgagcaag atcagctcca acgtcacccg cctgcagtcc
     7381 aaggacttca ccttctggca ggtggtcgag gacctctaca acatgcagat gggctcgaat
     7441 gtgaatccgg ccggtgagaa caccaactgg ggcaccgtgc tcatcgactt cgactgcacc
     7501 gtggctggca tggacggcaa gctctactcc gagcgccgcg tgaacaacaa cggcggtacc
     7561 accgatggac ttgaaccgtg cacggttatt gcaaacgcca agtacaacgg cgtcaaggcc
     7621 atcacccact tcatccacgc gggcgacaag gtcatcgcca acaacgatga ggacaacaac
     7681 atgactgcca cctccgacga tggcaagaca cagaccgtca tccaccgcaa ctccggcacc
     7741 tctgaccaga ccttcgtcat cgacctgtcg aagtacggcg agattgccga caacgcttac
     7801 ggtgagctct acctgaccac cgaaacctct gccgaagaca agaacgcggg tgtcgattcc
     7861 gccactccgg aagtcttcgc caagaccagc aacgtcaagc aagctgaagg ctctgtgatg
     7921 attgacaagg ctgccaagac cgctacggtc actgtgcccg cccgttctat cgcctccatc
     7981 cagctcactg gcgtgaccgg ctacgctaag gatgctgccg tcgagaccgg cgacacttac
     8041 cagctcgttg gtaagcagtc cggcaaggcc gtggctgata ccacttctgg tgattccgcg
     8101 ctgtccctgg ccaacgtcgc ttccgatgcc gagaacgcca agaagcagac ttggaccttt
     8161 acccagatcg agcagcccgc cgactccgag cgccctgatc tcaaggctta tgtgattact
     8221 aacgccgaag gcaaggtgct ggtgtccaag gatggcacga acgcgctttc caacgaaacg
     8281 gttgaggccg ctaagtccga cccggctgcc aagtggattc tcaacacttc cgatggttcg
     8341 acctaccagc tgctcaatgc cgcgactaag acgaacctcg atgtggataa ctctggtacc
     8401 acagtcggca cgaaggttgg cttgtggcag tcaccgagcg gcacttcgcc gtccgccaac
     8461 cagacatgga ctctacgcaa tgtaacgccg accagccaga agaccgtgaa cgtgcagacc
     8521 gccgttaacg agaaggccgc gctgccgacc gaagtcacgc tctactacac ctggggcgaa
     8581 ggcaaggcca cggttgccaa ctgggatact tccaaggtcg atgtggccaa ggaaggcacc
     8641 tacgaagcca ccgctaccgc caccgatgtg tacggcaacg agttcaatgt cgccgctacg
     8701 gtctacgttg gcgcgctcac cgtttccgat ccggtatcgg ctacagtgct ggccggcacc
     8761 agtgcgagcg aggcgaaggc cgcgcttgag gctgcgccgg tgtatctgca cgtcaaggca
     8821 tcgcctgcat tcgagggcga tgcggctaag gttacgtgga acttcgatgg gcttgatacc
     8881 aagctcgccg atgccaaggc tggcgacaac attgccgtga ccggtactta ccagctggac
     8941 gacgcgacca cgattgcgct gaagggcgcg atctatgtca ccgccgccac gcctgagaat
     9001 gtggccgaca ctgcttccag cctgaccgtg accaaccagc agacggaata cagcaagggc
     9061 gatcagtgga agaagctcac cgatggtgac acgtcagctg aagcctgggt gacgtggaac
     9121 tctgctggtg actattccgc cagcccgacc gccacgattg acttcggctc tgagtgtgag
     9181 cttagcagag tgaccattac gtatggtgac aaggctccgg cttccgccaa ggccgagtac
     9241 accactgatg gcgagacgtg gatgcaattc ggtagcgatg ttaagcctgc cgcaggccag
     9301 acggtgacgt tcaaggccga taagggcaca gtgaatgcca cgaaggtgcg cattgtgaac
     9361 accgtgaaca acgactacat gaacgccacc gaaattcagg cattcgtgac gccggttcag
     9421 ggtgctgcga agaacatcgc cgcggcctct ggcacgaact tctcggtgaa cttccaggag
     9481 ggtgcctccg cttccaaggc catcgatggt gacactacgt caaagggttg gtccacttgg
     9541 gcttccaccg cctcgacggt ggacccggtc gccacgttca ccttcgacga agctcagacc
     9601 atcaccgaag tgaagacctt cttctactac gatggtcgtg cgtcttggcc gaagagccag
     9661 acgctggaat accaggatga ggctggcgaa tggcatggag tcggtaccaa ggatggctgg
     9721 aagatacagg ccggcgatgc cggctctggc tccgacggca tcaccgccgc cgacaccccg
     9781 accgttgact tcgtgctcgg caccccggta aaggccaagg ccatccgcct gactaacaca
     9841 ttgcaggaca ccaaggtgta catcaacgtg gctgagatcc aggtgttcgc acaagacagc
     9901 acggtactca ccccgcagcc agcatccgat gccacgctgg gcgacctgcg tcttgacggc
     9961 gaaaccgttg aaggcttcga cccgtccaag accgactaca cggttgatct gccggtcgac
    10021 gccgaggcaa acccggtgct gcaggccttc gccaccgaca atgccgccgc cgtcaaggtg
    10081 actggcgacg cggttgagaa cggcaagctt ggcggcaagg ccgccattac ggtgacctca
    10141 gccgacgagt ctgagacgaa gacctacacg gtgaccttca acgccttcac tttggcttcg
    10201 ctcaaggtga tcggacccac gaagaccgag tacgccatcg gcgacaagct cgataccgcc
    10261 ggtctgaagg tgactgccgt ctaccagagt ggcgacaaga ccaaggaagt gccggtcgct
    10321 cttgacgacc cgcagcttgc gattggctcg ttcgactcca ccaccgccgg caagaaggcg
    10381 attaccgtct cctaccgtgg tgtgaccgcg accttcaacg tcacggtcaa ggccaacgca
    10441 gtcgcccctg gccctgaaga acagaagccc ggcaacacca acaagcccgg tgccaccggc
    10501 aacggcaaca agaacacggt ggccaacacc ggttccagtg ttgccgccat cgctggcgct
    10561 gtcgctctgc tggccgctgc cgcgggtgca ctgttcatgg tgcgcaagcg tgcatagcac
    10621 cctcgcgcag taatgggtga aggaacaagc cggcaatatt gactattgcc ggccggttcc
    10681 tccgctcttt gaccataatt ggtccgacaa gttgaatact gaattttcag tatcggtata
    10741 ttgatttgtc gaaccttttc tggcttcgcg gatagcgaca taactgtttg ttcaaaaaac
    10801 cgttatcatc aatcaaggaa ccaaatcaat gaagataaac aataagggca agggcgctct
    10861 tatcgcggca attaccgccg cggcaacgct attgtcatgc gggctggccg ctgcaagtgc
    10921 cagtgcggca ggtgtggatt acctgcctac catcggccaa gtgccgacat acaccaagtt
    10981 ccagcccaca gccgatccgg gcaagaacgc tagcgattac ttccagccat attggtatgc
    11041 caagaacgcc aatgataatg gcggcacaca catccaagcg cacggtggcc aagtggtcaa
    11101 ggtcggcgac gcctactact ggtatggcga agaccgttct aacggttacg acaacagccc
    11161 cggtgtccat gcttatatgt cgacagatct atacaactgg accgatcttg gtgtggcgct
    11221 gcgtgcggtg accagcaaat ctcagttgac ggataagagc aatgccgatt acgcctactt
    11281 cgacaaggcc tacaacctga ccaagtccga cggcagtgtg gacgctgcca aggccgacgc
    11341 aatcttcccg tacctcaaca ccaaccccga tcaggatggt gatggcgcgg ttgattccgt
    11401 acagggcatt ttcgagcgtc cgaagatcat ctacaacaag aagaacaagc aatacgtgct
    11461 gtggtggcat tccgatggaa gcaccacgcc gggcggttcc aactatgcac gtgcacttgc
    11521 gggcgtggct gtttccgaca atccggcggg cccgttcact atggtgggtg cctatcgttt
    11581 gcctaaccag aacaattgga aagaagccgc aggtaacccc agctggggtg agaacggtga
    11641 cagccgcgat atgactgtgt tcgtggatcc gaaggacgac agtgcctatg tactgtattc
    11701 ttccgaagcc aatgccacgc tgtatatcgc caagcttaac gatgattaca ccaatgtagt
    11761 caagaccacg aatgtggacc agtccgaggg acaaaagcag tactctgctg acgggcagta
    11821 cccatacatt cttgcagacg gtactacgga tgccccggtg cgtggcgaag atttccaaat
    11881 cgtcaaacaa aatggttcgc tggaagctcc tgccgtattc caatatgacg ggcgttacaa
    11941 catcatcgca tctggtgcaa ccggctggga cccgaacaag cagacctact acaccgccga
    12001 ctccatgctg ggaagctgga cccgtggcgt ggaaaaggac gatgccaacg agaacacgtg
    12061 gtacaacaac atgccggaag gcgcggatgg tctgttgtct gtgggcgata cccgcggcac
    12121 cacattcggt tcgcagtcgg ctagtgtgct cgcagtagac caggagaaag gtcacttcat
    12181 ctaccttggt gaccgttggg attccggtaa agccgattcc acctatgttt ggctgccgct
    12241 gaccatcggt gagaacggca ccatcgaaat gcataatcct gctcaagaag gcgagcccga
    12301 cggctgggat ctgaactatt gggacaacca tggtagcgcc aagggcaagc tggtcaactg
    12361 gactgtggaa accggcgatg atctcccgaa gaccgtgaac acgggcggaa ccgttactct
    12421 gccggacacc gtcaacgtca aggaaggcga cgataccatt gctaccaagg tgacatggaa
    12481 tgtggaaggc ggtacggcag tcagcaagtc gaccaaggct gccggcagca cctacgcatt
    12541 caatgtgccg ggaacctaca ccattacagg cactcttgcc gagagcagca acttcaaccc
    12601 gggccgcaca ttccgcagaa ccatcgacgt ttcctgctcc aacccgattt ccggaagttg
    12661 gaaggaagcc cattggaagg gcggcagcgc gtgccaggtc gccgcatccg gcggtaccta
    12721 tgacttcacc gttacggaca acgccaatcg tggtgtctgg acggatcgca acgagggcag
    12781 cgcggtgtac cagcctgatg ctctggacgt gaacgaaacg ttggaaacca cggtcaagcc
    12841 gctcgacttg ggcggtaatg gcgatccgcg tgccggcctg gtggttcgta acggtcttgc
    12901 tgacgctaac ggcggcaagg gatatgccnn nnnnnnnncc gataccctga aaggctactg
    12961 gcgtgcttcc gcgaacgatg aatggcagga tgtcgctacg gtaacgctga ccggtgcgga
    13021 cgcaaccggg ctcgatgccg gtgtcttcgc cacgtcgaac agcaatgccg gcgcattcac
    13081 cgtggccttc aacggcactg cgttcggttc gcagactgct gctgtggagt ccatcgcggc
    13141 taaggaccct gaagccacta tcgccaaggg gcagatgctc gcgcttcggg acgtgacggt
    13201 taccgccacg ctcaccaatg gcaaaacgcg tgtgctggag ccaagcgaat acacgctgga
    13261 aggcttcgac accaccaaat tgggcgagca aaccgtgacg gtgcgtcttg tcgctgattc
    13321 ttcagtaacc gccacgttca ccgtgactgt ggaaagcaac cttgcccggt tgttctgctc
    13381 gtccgctgcg gcctcgaagt atgagccggc cagtagctgg gcctccgctt ctacggccga
    13441 cctgacttgc gacaacaacc tgagcaccaa ctggtcgaac tggggcaccg gcgacacctc
    13501 gccgtggctc agctacacct tcgataaggc atatcagctg ggcaagctca gcgttgcggt
    13561 ggataaggcc aagggcgagg ccgctccgaa gagcttcact gtatcgtacc tagctgaaga
    13621 cagcgccacg tggactgatg ccacgctgcc ggcagtcact gtgaatggtg ctgctggagc
    13681 cgtgacggaa gccgatgtga gcgctctgcc cgccaccaag ggcattcgcc tcaacttcac
    13741 ctacgccgat ggcaatgact atgccaagat cgctgaagta cgcatcgccg aaggtgaagc
    13801 gacaccagag ccgcagccgt ctagtaacgc caatcttgct gatctgactg tggatggcaa
    13861 gacggttgac ggattctccg cggataccac taaatatgcc ggtgcgctgg ctggagatgc
    13921 tgcttcctac ccgacggtgg aggcgactgc tgctgacgca aaggctacgg tgcaagtgga
    13981 gcaggcttcg gccgagaaca acggcgtggc cacggtgact gtaactgctg aggatggcac
    14041 ggcgaaaacc tacacagtga cattcggcga actgcctcag ttggctgagc ttgctgtgga
    14101 agtgaccaag gattcctatc aggtaggcga taagttcaac gctgccgatg tgaaggtatc
    14161 cgccatttac aaagtcggcg atactgaaac gctgcgcaag ctgattgatc caactgatgg
    14221 tgatctgaag ttcactggct ttgattctgc caccgcaggc acgaggacca tcaccgtctc
    14281 ttatcgtggc gtgaacgcga cgttcgaagt cacggtcacg gccacggagg tcactcccgg
    14341 ccctggagag cagaagcccg gcgataccaa caatcctggc aacactgcta agcccggtag
    14401 cactgccccg aataagccgg ctgctaatgg cgctgcgccc ctttcgaata cgggtgctgc
    14461 cgtggctgcc attgcggtcg tggttgtggt gctggcagct gcggctggtg ccttgctcgt
    14521 catccgcaaa cgccgcgcat aaccatggca actgctgagt agcgtggatt gcgttgctat
    14581 tgagcaatct gcgattcacg ccactcagcg agtgcacccg agtgcgcccg agcttgcgca
    14641 atgcaggcgc aatacagtgc aaacagtaca aagcaacaat aatggccgnn nnnnnnnntg
    14701 ctccatgcaa gggcagctgg ctcagaccgg ccattgtgtc tgcaagtaca tgcaattaga
    14761 gcatgaacat gtggaagccg ttgagcgtta ctggttgttt tgctttgttg ttgcgctcag
    14821 cagtgcgacg gacgtccgcc cgtagtgtag tgcagcgctc cgtgaaccag tgccgatgtg
    14881 aaatgcatgt tgcacatgcc cgcttcttct gcttcgtgcg tgcagccggc gatgctgcat
    14941 aggttcatac cccgaatcat ggtcataccc cctctttggg atggcatccg tgccgccatc
    15001 tgccttcaga gcatagcgga caattgttgc attgcctagg cggtacaacg ctttctgtgg
    15061 cctgtatcac atccttgccg gcgttctgcg gcgtggttgg gcgtgtacat gtgcacttgt
    15121 gcggttttgt tgtgtgtgat ttgggtatac ggatagagtt tcttctctgc agtggcaagg
    15181 gcctctcggc cggcgagccg gctgatctct cccatgcgtt gctcaatgat ttccgttttg
    15241 cgagtctgaa cggcaaaata gctctggaga agtgccactt cttctttctt tggatcaccg
    15301 ttttgagcaa tgagatagca ggcgtatcgg gagtgctgag ttcgctggtt ttgcgtgtga
    15361 tttcgtcgcg gttcatgggt gtgtccttat gtttttcatg accattttcg tgactccacg
    15421 aaaatggtca aagccaataa ttgcaatggt tttgaggtat gggcgcgctc cgcccaactg
    15481 gtctgtcttc gtccgccttt tcgaccagat acctcatgga gcacaatgcc aacgtcgcta
    15541 ccgttggccc agcaaatatt cttagacgac gccccgaagt gaatatctat gcggctttca
    15601 gaatatcaaa gagcgtggag tgtgtgcatt atgcgttcca aaaatagttt ctagtaatga
    15661 attgtttacg gtgtgagata ttcttggggt gtttgcggcg ctctgccgtg tggcgaatgg
    15721 gaatggatgg tgctcttatg ggatttgagc aggaagcagt tagtgaactg tatgccagcg
    15781 tgtggggcaa tcgttcgaca atgcagaggg aattcactcg tggagcccat ggtgagcagt
    15841 tcgtgctccg ggagctgtcc atgaagggga cgcaaacgcc atcgcaattg gcatcggcgc
    15901 tgcaggccag ttccggccgc atctccactg tgctgtcgtc tctggagaaa aaaggctggg
    15961 tcacgcgtga cattgattcc aaggatcggc gcattattcg cgtgaatctc actgattccg
    16021 ggcgcgagca gtctcatcgg atgatagagg aaatgcgttc cgcaatttgc tggattttct
    16081 cgcaaatggg ggagcggcgc acgcgcgaat tcgttgacct ggtgtctgag ttcaccatat
    16141 atatgtcgat ttgccatccg gggcggcctc ggccgacggc tgaacaagtg cgtgaggcat
    16201 tcgtggagcg ggacaagcgc gtggctgaac acatggcagc taagcgcgcg gagaactaat
    16261 cggcaaggct aattgaggac gctcacagcg aacatgcaga agtgcgcgta atatctcaca
    16321 gtgtaagtaa tcaaacaatg gaaactatta agacatcgtg aaccgataat caccgataac
    16381 ccagccaact taagaaccaa tcgccaataa ccaataacca acccaaccaa cacaagggga
    16441 aaccaatgtt tcgcatcatg aaatacctct ccaaagcgga gatcggtcag atgctcatcg
    16501 ccctggtgac catcgtcggg caggtctact tcgacctgaa actgcccgac tatatgtcag
    16561 acatcaccac gctggtcgag acgccgggca gcgacatgaa ggacatctgg atcgcgggcg
    16621 gcaagatgct gctcatctcg ctcggatccg tggcgtgtgc catcatcacc ggctacatcg
    16681 ctgcgcgcgt ggccgcctcg ttcacccagc gtctgcgctc gctcgaattc cgcaaggtcg
    16741 agtccttcgg cccagccgag atgagcaagt tctccaccgc cagcctcatc acccgttcca
    16801 ccaacgacgt cacgcaggtg cagatgttca tcaccatggg cctgcagctc atcgtcaagt
    16861 cgccgatcat ggccgtatgg gccgtgtgca agatcgccgg cgaaggtttc gaatggacgc
    16921 tcgccaccgg catcgccgtg gcggtcctgc tggtgtccat caccgtcatg atggccatgg
    16981 tcatgccgaa gttcaaggtc atgcagaccc tgaccgacaa catcaacctc gtggcccgcg
    17041 agaacctcac cggcctgcgc gtggtgcgcg cctacaatgc cgaggactac caggaagcca
    17101 aattcaccaa ggccaacaag gacctcaccg agacccagct gttcaccaac cgcgtcatgg
    17161 cgttcatgat gccgttgatg aacaccgtcc tgaacggcct gatgctcgcc gtctactgga
    17221 tcggcgctta cctcatcgac gccgccgggc tcaaggacaa gctcaccgtg ttctccaaca
    17281 tggtcgtgtt ctccaactac tccgtgcagg tcatcatgag cttcctgctc atgagcatgg
    17341 tgttcgtgct ctggccgcgc gccgacgtgt ccgcgcagcg catcatggag gtactcgaca
    17401 ccgagccgat cgtcgagaac ggcaccaaaa ccgccgccga cgtggccaaa accggtcagc
    17461 gaggcaccgt ggaattccgc aacgtcagct tcacctaccc ggattcgcgc gaggcgatgc
    17521 tggaaggcat caacttcacc gccgagcagg gccagacggt ggcgttcatc ggctccaccg
    17581 gctccggcaa gtcgtctctt atcaatctcg tgccgcgctt ctatgacacc agccaaggcc
    17641 aggtgttggt cgacggcgtg gacgtgcgcg actacgacct gaaggccctg cgcgacaaga
    17701 tcggctacgt gccgcagcag tccgtgctgt tcaagggcac cgtggcctcc aacgtgagct
    17761 acggcgatca gccgggcgac ccggcggagg tcgagatggc cgatacctcc accccggccg
    17821 ggcgcaagcg cgaggccgcg ttgatcgccg ctggcgaggc cgcggaaggt gccgatatac
    17881 cggccgagca gctgaatcgc gtcagggccg ccgccgacgt ggcgcaggcc agcgagttcg
    17941 tggcgcgcat ggacggcggg tattccgccg cgatcgccca aggcggatcg aacgtgtccg
    18001 gcggccagaa gcagcgcctg tcgattgcgc gcgcggtgta ccgtcacccg gaaatcctga
    18061 ttttcgacga ctcgttctcc gcgctcgact tcaagaccga ccgcgaagtg cgcgacgcgc
    18121 tcgcccgcga ggccaaggat tccaccaagc tcatcgtggc ccagcgcatc ggcaccatca
    18181 tgaacgccga ccgcatcgtc gtgctcgacg acggcaaggt cgtgggtcag ggcacgcaca
    18241 aggaactgct cgacaactgc gacgtatacc gccagatcgc cgaatcccag ctcagccagt
    18301 cggagcttac ggcgtagggg ggggcggcag cggtagctcc ccatgtcagg ggctgagccg
    18361 taaagggaac agcccagtgg gctgttccca ggcgaagacg agctgataag cgagcgaata
    18421 aaacagcaca gctgactgag gggtagtcgc gtacggcttc tctccctccg gcgcttcgcg
    18481 ccacctcctt catcagaggg aggtcaatac ggacttttaa ggagtaaaac aaatgccaag
    18541 aggaccaatg ggcggcaggc gtgccgtcga agagccccat gatttcggca aagtcatggg
    18601 caaactcgtg aactactgcc gcaactatct tcccgtcatc atcatcgcgc tcatcctggg
    18661 cgcggccggc accgtctgcc agatcgtcgg cccggacaag ctcaaggaca tgaccaacga
    18721 gatcgtcaaa ggtctgcccg ccatcgttaa cggcagaccg gtgatgaact cggtagattt
    18781 cggcacggtg ggccacatcg cctggacttt ggtggccctg tacgtgggct acgccgtgct
    18841 cggctacgtg caaagctgga tgatggccaa cgtcacccag cgcaccgccc agcgactgcg
    18901 cgagtccatc tcggtcaaaa tcaacaaact gccgctcaaa tacttcgaca aggttagcta
    18961 cggcgacgtg ctgtcgcgca taaccaacga tgtggacgcc atcggccaga cgctgggcca
    19021 gtcgctcggc tcgctgatta cctcggtgac gctgttcgtg ggcgcgctgg tgatgatgtt
    19081 ctacaacaac gtgatcatga ccctgtgcgc catcggtgcc agcctcatcg gcctgatcat
    19141 catgatggcc atcatgaagt cctcccagaa gtacttcgcc cggcagcaga tggcgctggg
    19201 tgacgtcaac ggccacgtgg aggagatgta cgccggtcac ctcatcgtca aggcatacaa
    19261 cggcgaggcc gattcgattc gtcgcttcga gaagtacaac tcggacctct acgagnnnnn
    19321 nnnnnggcaa cttcggctac gtggtggtgt gcgtggtggg cgcggcgctc gcgctcgacg
    19381 ggcagatcga gttcggcgtg atcgtggcgt tcatgatgta tgtccgcctg ttcactcagc
    19441 cgctttcgca gtttgcgcag gccttccaga acctgcagcg ctgcgcggcc gccgccgagc
    19501 gcgtgttcgg tttccttgaa gagccggaga tggaagacga atccggcaag caggccctac
    19561 tcggcaagaa ctacatgggc ggccacgaag tgaagggcga cgtggaattc agccacgtac
    19621 agttcggcta cgaaccgggc aagccgatca tccacgactt ctccgcctcc gtcaaggccg
    19681 ggcagaaggt cgccatcgtc ggcccgaccg gtgccggcaa gaccaccatg gtcaacctgc
    19741 tcatgcgctt ctatgagatt tccggcggct cgatcgcgat tgacggcgtg gacaccaagt
    19801 ccgtgccgcg ctggaacgtg cacgaccagt tctccatggt gctgcaggat acgtgggtgt
    19861 tccgtggcac tgtgcgcgag aacatcgcct actccaagcc tggcgtgacc aacaagcaaa
    19921 tcgaggatgc gtgcaaggcc gtgggcctcg accacttcat ccgctcgctg ccggacggct
    19981 atgacaccgt gctcgatgac aagtcgtcgc tctcccaggg ccagaagcag ctgctgacca
    20041 tcgcccgcgc gatggtgcag gacgcgccga ttctgattct cgacgaggcc acctcctcgg
    20101 tcgatacccg tactgaagag ctgattcaga aggccatgga tgcgctgacc gtgggccgta
    20161 cgagctttgt gatcgcgcat cgcctctcca cgattcgcga cgccgacatg atcctcgtca
    20221 tgaaccacgg cgacgtgatc gagcgcggca cgcacgacga actgctggcg gcgggcggtt
    20281 tttacgccga cctctacaac agccagttcg cgctcgctga ctgacggatt gtttcctgcg
    20341 ttagcggatt agcggattag ctatactcgg ctcccctctc nnnnnnnnnn gaggggagct
    20401 gtcagcgtag ctgactgagg ggagcaaccc atcggtccac ccgagggaat gctccactgg
    20461 atgtgatact gatgatttcg tacattagtg tgtgaaaaag ccgccgggaa aattggggga
    20521 ttgggcggcg gtgcggttgg cgttgagaat ggcgcattcg aaggggaagt gagcgcgcag
    20581 ctgggtggcc ggggtggtga acagggctgt ggcgagtccg tcggccagtg tgacagggac
    20641 gtgtgctgca acggtgttcg acgaatcgtc ggtatcgcgg tttgccgttg tcgaggcatt
    20701 gctgccatgc gtcgatgtct tcgtggttat ggcgctcgat ggcggcgtga cggccaccca
    20761 tgtggcggct acatcgtcaa ccggtagtcc gtcgatggcg ttgagcagat ggtgaagttg
    20821 gtgacctgcg gcgtcagacc agtggcggcg gctgggggag ctcgcgcaga atgcgccgtg
    20881 gctgatttcg accgtaccta cggcgttggc cggatcggca ggatcttcaa gggcgatggt
    20941 gattggttcg tccgtgtgca ctagcaaatc acccccggcg tcgatgacgt attgaatggg
    21001 ttgagggtga cccgcgccat cacccaacat ccccgcaagc aaatccacca aatacccctt
    21061 accgcacgcg ccgaaatcga gcgcgaccgg tccgcgcgtc accagtgttg tgccatgccg
    21121 ctcaacgtcg gctgaccaaa tggctcgtcc gtggatcgcg cctgcatgtt cagcggcatc
    21181 tggcgcaggc gtaaacgaat acgactcgtc atagccaagc cgaatcaaat cctcgcccac
    21241 gcacgggtcg atcgccccat cggttgccgc aagcaggcgg tcgtacaggc caaacagacc
    21301 cgatgcccag tcgggaaaat cgaacgtgcc gccatgtgcg gccgtgcgca tccggccgac
    21361 gatggaatca tcgcggaatc gagataatac aacctcatat tcatggatga acgagccaat
    21421 acgatcgcgt atctcatcgt ccaatacgct ctcggtgtgc accagtaatc cggtccccaa
    21481 cgcatcagga aaagcgtata cgtatggcag ttgctcggtc agacttgtca tgcccactat
    21541 cgtagagcgc atggtgtgcc ggtgtatact ggattttgac actgtgtcaa tgttgattcg
    21601 gaggttgtga gaatgggcaa tgcgactaca gcaagtgtaa cgggtgtgca agttgcaccg
    21661 gcgaagtcag ccatcagaga tctccccatc gccaaagtgt gggcgtttgc ggtcggccag
    21721 ttcggctggg cgctgctttc cggcattatc tccaactggc tcgtgtattt ctatcagccc
    21781 gatcaggaga ccatctccca aggtcagacc gtattcgtgc cgcaaggcct ggttgtgctc
    21841 ggcatcgtga ccgtggtggg cggcatcacc gccttcgccc gattcttcga cgcgttcgtg
    21901 gacccggccg tggcgtctct ctccgaccgt tgcgacgcca aatccggccg ccgtatgccg
    21961 ttcctgaaat tcgccgccct gccgctcgcg gtggtcaccg tgctcgtgtt ctggagcccg
    22021 atcaacggca ccagctgggt gaacgcggcg ttcctgttcg tcaccgtgat cggctactac
    22081 atcgccctca cgttctactg cacgccgtac aacgcgctca tcgccgaact cggccacgac
    22141 tccaagcagc aactcaccat ctccaccgcc atctccttca cttgggtggc cggcacggcc
    22201 atcgcgtacg tggcgccggt gatttggggc gcgttcgtgc cgatgatggg ccgtatcacc
    22261 gccatccgcg tgaccttcac catcatggcc gccgtggcgt tcgtgtgcat gctggtgccg
    22321 ccgctggcca tccgcgagaa ggactacgtc aactcccatc ccacctccga atcggccatc
    22381 gaatccctga agcagacgtt cggcgacggc gaattccgca agttcgtgtg ttccgacgtg
    22441 gtctactggg tggccatcac caccttccag accggtctgc cgttcttcgt gaccagcctg
    22501 ctcaagctgc cggaaaccac cacgaccatc tacttcgtgc tcatgaccgg cgtctccgtg
    22561 ctgttctatc tgccggtcaa tatcttggcc aataaggtcg gcaagaagag gctgctgctg
    22621 gttgccttcg tgatcttcac ctgtgcgttc gcatttgccg gcgcccttgg ttcggccgtg
    22681 ctcggcttca ttccggcgat ggcccagggt
//
LOCUS       DAWBMW010000013        21577 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_662_length_21577_cov_2.937181, whole genome shotgun sequence.
ACCESSION   DAWBMW010000013 DAWBMW010000000
VERSION     DAWBMW010000013.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 21577)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 21577)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..21577
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_662_length_21577_cov_2.937181"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3489..3498
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6110..6119
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6746..6755
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10309..10318
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11214..11223
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    15249..15258
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4397 a         6585 c         6209 g         4326 t
ORIGIN      
        1 ccacctcgaa ctgcaccgag taccaggctc gccgcctgaa catccgcgag cgtatggaag
       61 acggcggcac ccgcccggtc tccaccctga acggcacgtt ggccaccacc cgttggctgg
      121 tcgccatcat ggagaaccac cagcagaagg acggctccat cgagatcccg aaggccatgc
      181 gcgcctacat gggcggcaag gaagttatcg agcccacgaa gtgggaggcc tgagtctcat
      241 cgtgctttag ctccctctga tgagggagct gtcagcgtag ctgactgagg gagagattac
      301 tgagggagag atcgctgata gccagcaggc gaatccaaga ccgaccgcac tgccgaatat
      361 caacatccct cccttctgta taatgaatac ttgcgcgtgt gaacgcgcat caggacaggt
      421 gtctgagcgg cctaaagaga cggtcttgaa aaccgttgag gggcaaccct ccgcgagttc
      481 gaatctcgcc ctgtccgcga accgaggcct gggcaacatg cccaggccct ttttttcttt
      541 ttctggaacc tgtcttcttg attgccgccg gggtgctgat atattggttt gtgatgccgt
      601 aacatgcggt gtccccgttg atatgacggg ccgatcggcg tgagccgata agaaaaggcc
      661 cgacgtttgc agcaccgggc ctttgcgttg attaccggct tgcgccttta atcaacaagg
      721 ttgcaagatg caatcatatc acgatgcacg gtagtctgac cgtgaacggg cgcaccgtga
      781 tcgtccatgt gggcgatggc gaggcgaacg ccacggttga cggcacgcac ttcaacgtgc
      841 gtagcctgtg gcagctctac cagttgctga gactgctcgt gtagcgtctc attaaggcgc
      901 ggtgcctccc tagtcgggta tcgcgccggc gacctgaaaa ccgataatgt tggcaagtgc
      961 ttgcaatttg cggtggacga ttgcaagcta acgtcttgga ttgttgccgc tatccaatat
     1021 ctcattaggt ctaccggcgg accaaaagta cgttgctttg taaggggagt taatgaaata
     1081 aacaaatccg cgcaatcgag catcgaaccg gttcaatgct actcgcctca acgggacgcg
     1141 cggtaattgt tagctaagtt gacaataaaa gaatgagggc atacattagt aatcagtcat
     1201 cacactgaga tgatgatttc actacgaagg agtagagata tggtatctcg caataagcgc
     1261 atcgtggctg cttttgccgc ggtagcagca atgggaatgg gcttggccgg ttgcggcagc
     1321 gacactgccg gcgacacgaa gaccaccgat gatggtggcg tggtcaacat cacctacatg
     1381 caccgtctgc cggattccga gggcatgact ctggtcaacg acatcgttgc caagtggaat
     1441 aagcagcatc cggatattca ggtcaaggcc accaagttcg atggtaaggc ctctgacatg
     1501 atcaagaagc ttgagaccga cgtcaagtcc ggcgaggctc cggatctggc tcaggtcggt
     1561 tacgccgagc tgcctgaggt cttcaccaag ggtctgctgc aggatgtgac ccagtatgcc
     1621 gagcagtaca agaacgactt cgcatccggc ccgtacagcc tggttcaggt tggcggcaag
     1681 gcttacggcc tgccgcagga caccggcccg ctggtttact tctacaacaa ggctgagttc
     1741 gagaagctcg gcatcaccga gattccgcag accgccgatg agtttatcgc cgctgccaag
     1801 accgctgccg ccgctggcaa gtacatcatg tcctaccagc ctgatgaggc cggcaacatg
     1861 atctccggtc tggctggcgc ctccggtggt tggtacaagg tgaagggcga ctcctgggtc
     1921 gtcaacaccg agaccgatgg ctccaaggca accgctgact tctaccagca gctgctcgac
     1981 gccaaggcag ccaccaccaa cccgcgttgg gatccgtcct tcgatgcatc catcaaggat
     2041 ggctcgttga tcggtactgt ggccgccgct tgggaagccc cgctgttcat gacctcctcc
     2101 ggtggcaccg gctccggcga atggcaggtc gctcagctcg gtgactggtt cggcaacgct
     2161 ggcaagaccg gccctgacgg tggttccgcc gtggccgtgc tgaagaactc caagcacccg
     2221 aaggaagcaa tggagttcct ggattggttc aacacccagg ttcctgatct ggtttcccag
     2281 ggcctcgtgc cggctgctac cactgaagac gctgagactc cttccgagtg gtccaccttc
     2341 ttcggtggtc aggacatcat gaaggaattc aagaccgcta acaacaacat gggtgacttc
     2401 acctacatgc ctggcttctc cgcagtcgcc gccaagatga acgaaaccgc cgccaaggcc
     2461 accgatggct ccggcaaggt tgcagacatc ttctccgacg cacagaccac ctctgtggat
     2521 acgctgaaga acttcggcct gtctgtttcc gagtgacatt aacggttagg gttccttcca
     2581 gccgctgata tcctcaacca cacatcgaaa ggccggagct caacactccg gcctttcctt
     2641 ttactcgagt tcggttcgtc attaattgtg tttgatctga ctgcatcgat tcgaactgca
     2701 ttacgtactt tctatttctc actgttcttt tgaaagacag gtttccctat gactgcaaca
     2761 acaaaaacgg cgaagacggg ggtcgccagg cgtgccgata agccaaaggc ttctgatgct
     2821 gtgaagcgtg agaatcgtac cggctgggcc ttcatggcac cgtttggcat cctgttcgct
     2881 ctggtattta ttcttcccat catttgggct atttattcca gcttcttccg ccaggtcacc
     2941 acgggcggtg gcgcatacgg cggcggggaa ctggtcgata agttcgtcgg acttcagaac
     3001 ttccaatacg ttattacttc cggcaacttc tgggctggcg tgggcagggt agtgctctac
     3061 accgtgattc aggtgccggt catgatcatc gcggcacttg ctctggcaat gctcatcgat
     3121 tcctatatcg tcaagcacat caccgcattc cgtctgagct acttcctgcc gtacgccatt
     3181 ccgggcgtgg tcgcatccat catctgggtg tacctctaca acggccagat ttcccctatc
     3241 gttaagggac ttgccgccat cggcatcaag gtgaacttct tcggcgacaa tgtggtcctc
     3301 ggctctatgg ccaacatcac gacctggacc ttcaccggct acaacatgct gatcttcctc
     3361 gccgcactgc aggccattcc acacgatctg tacgaggccg cacgcatcga tggcgccagt
     3421 ggcttccaga tcgccatgaa gatcaagctg ccgaacgtgc gtgcagccgc cctgcttgcc
     3481 atgctgctnn nnnnnnnnac gagccgcagg tgatgtcgat ttccgatccg agcatttcca
     3541 agtcgtacac gccaatgatg atggccctca atacctcgca gggcacgctg accccgggtg
     3601 gtgacggacc tgcatccgct gtggccatcg ttatggcact gatcgctggc gtactcgccg
     3661 tggtctacac gctcgttgaa aggaaggtga acgcagaatg agcaccgtag aagctactga
     3721 taaggaatcc cgcgcggtcg cacgcgctcg taagaaggct gacaggattc gcgacaagca
     3781 tgccaacgac ctgcctcgtt ccatgcagcc ttccgcgctg gtcaagacca tcaccatcgt
     3841 tgttctggtc ttcgcgctga tctactttct gttcccgatt tactgggcca tcatcgcctc
     3901 gaccaagacg ccgagccaga tgaccggcag caacggtctg tggttcgccg tgggtctgag
     3961 cgatttgcct gccgccatcg ccaagaacta tggcacgctg attggttgga cccgtggcca
     4021 gttctggcgc tgggtgctga actccctgat ctactcgggt gtttccgctc tggtgggtac
     4081 gctcgtggct gtgatggcgg gatacgctac cgcaaagttc aacttcaagg gtaagaacct
     4141 tgccattggc gtgattatgg gctgcatgct gatgccggcc gctctgctga ccattccgca
     4201 gtactccatc ttccatgcca tgcacctgac caacacgatg ctctccatca tcatcccgtg
     4261 ctgcgtctct ccgttcggct tcttcctggg ccgcgtatac gcgcagacct cagtgccgaa
     4321 cgagctgctt gaggcggcgc gtatcgatgg tgcaagcgaa gctcgcatct tcttcaccat
     4381 tgtgttgagg attctggctc cggcaatggt cacgatcttc ctgttcctgt tcgtggccac
     4441 ttggaacaac ttcctgctgc cgttgatgat ggtctcttcc gacaccttga agccggtgac
     4501 cttgggtctg tacggcatgg tctctctggc taccttcact gatcgtggcg ctctgatgat
     4561 gggtgctctg ctcggtgtgc tgcctgtgat cgtgctcttc ctcggtctcc agcgctactg
     4621 gcaggccggc ctcgccgctg gtgcggtcaa gggctgacgc gttaagcgga cagcccggtg
     4681 ggctgtctgt agcgtcggcc cgagcgaggc ggtagccgag cgaaggcagc attgagtctc
     4741 gccgtaggcg agtccttaat tgcaggggcc gaaggcgacg cgttaagcgg acagcctaag
     4801 accaataatt cccgccgtcc ggtgcctcgt gctactcctt ccggcgccgg acggcttctc
     4861 aacaataagt agcacgcaac cataccgatc cagagccttc ctgattcttg tcatgagatt
     4921 gtcggtgaaa ctgaccggga agctccggtg atgttcaatt cgctcttccg gcgttgccgt
     4981 ggggctactc tcaaggattt atacagtcag gatcctggac gcccgacggt tgtgtcggat
     5041 tcttgtttta tatgcatata ttgtttacaa tttaattatc caaaaaaata atcaatgtcc
     5101 tcaaaggaga gactatgact agcaccggcc gcttcacctt gcctagcgag gagaattttg
     5161 cggagaagac caaggagctt gccgaactgt ggggagcaga cgccattcgt aactccgatg
     5221 gcacgcatct ggatgaggct gtgcttgcct tgggcaagaa gatttacaat gcgtatttcc
     5281 cgactcgcgc gcataacgag tggattacgc tgcatatgga tgagacgcca caggtgtatc
     5341 tgctgaccga tcgcattctc gccgaatcgg atacggttga tattccgctg atggaaagct
     5401 tcttcgctga gcagctgaag ccgaatcgtg atgccgaccc tcacaagtat tgggaggttg
     5461 tagaccgcac caccggcgag gtcgttgatt cggcgaactg gacgcttgac gcggacgagg
     5521 acaccgtgca tgtgagcggc gttgccgcat ggcatgagta cacggtgagt ttcctggcct
     5581 acatcatctg ggacccggtc gagatgtaca accatctgac caacgactgg ggcgacaagg
     5641 aacacgagat cccgttcgac atctaccatc cggccacccg caagttcgtg ttcgacacgt
     5701 tcgagcagtg gcttaaggac agcccgcaga ccgatgtggt gcgattcacc acgttcttct
     5761 accagttcac gctcctgttc gatgaaaagc gccgcgaaaa ggtcgtcgac tggtttggtt
     5821 gcgcctgcac cgtaagcccg cgtgcgctgg atgatttcga agccaagtat ggctaccgcc
     5881 tgcgcccgga ggacttcgtg gacggcggtg cctacaactc cgcatggcgc gtgcccagaa
     5941 aagcccagcg cgactggatc gacttcctct ccggcttcgt gcgcgaaaac gtcaagcagc
     6001 tcgccgacat gtcccacgcc gccggcaaag aggcgatgat gttcctcggc gaccagtgga
     6061 tcggcaccga accgtacaag gacggattcg acgaactcgg cctggacgcn nnnnnnnnnc
     6121 caccacccgc atgatcgccg atattccggg cgtcaagtac accgaaggcc gcttcctgcc
     6181 gtacttcttc cccgacacgt tctacgaggg caacgatccg agcatcgaag gcctcgacaa
     6241 ctggcgcaag gcccgccgcg cgatcctgcg ctcgccgatc agccgcatgg gctacggcgg
     6301 ctacctctcc ctggccgcca agttcccgaa gttcgtggac acggtgaccc atatcgccaa
     6361 cgaattccgc gacatccacg accgcaccgg tggggtggcc gccgaaggtg agctcaacgt
     6421 ggcgattctg aattcctggg gcaagatgcg ctcctggatg gcgttcaccg tggcacacgc
     6481 cctgcccaac aagcagacct actcctacta cggcattctg gaatccctgt ccggcatgcg
     6541 cgtgaacgtg cggttcatca gcttcgacga cgtactcgcg catggcatcg atagcgatat
     6601 cgacgtaatc atcaacggcg gcccggtcga caccgcattc accggcggcg atgtgtggac
     6661 caacccgaag ctcgtggaaa ccgtgcgcgc ctgggtgcgt ggcggcggcg cgttcgtggg
     6721 cgtgggcgag ccgagctcgg caccgnnnnn nnnnncagct ggccgacgtg attggcgtgg
     6781 acgaggagcg ctatcagacc ctttcggtgg acaagtattt cccgccggtg gtgccggacc
     6841 acttcatcac cgccgatgtg ccggtcgacc cggcggcccg cgaggcttgg gagcaggccg
     6901 gctaccgcat cccgctgagc ggctgcggcg gcggccagtc catcaagccg ttgggcggca
     6961 tcgatttcgg cgaacccgtg ctcaacacgt atccggtcaa tgaaaacgtg accctgctgc
     7021 gcgcggacgg cggccaggta cagctcgcga ccaacgatta tggcaagggc cgcggcgtgt
     7081 acatctccgg cctgccctat tcggcggcca acgcccgact gctggaacgc gtgctgttct
     7141 acgcttccca caacgaagac aagtatgcgg cctggagctc gtccaatccg gaatgcgaag
     7201 tggcacactt ccccgagcaa gggctgtatt gcgtaatcaa caacaccgat cagccgcaga
     7261 agaccacggt gactttggcc gacggcacca ccgaggactt cgacctgcct gacagcggta
     7321 tcgcctggcg tgaagcctga tcccacgttt tggagaacgg acccatcatg actgaaagca
     7381 atgaagtttt attcggcatc gcctcgcatt ttgcgctgga aggtgccgtg accggtatcg
     7441 aaccttacgg agacggccac atcaacacca cctatctggt gaccacggac ggcccccgct
     7501 acatcctcca gcagatgaac accagcatct tccccgatac ggtgaatctg atgcgcaatg
     7561 tcgaactggt cacctccact ctcaaggctc agggcaaaga gacgctggac attgtgccca
     7621 ccacctcagg cgccacctgg gccgagatcg atggcggcgc atggcgcgtc tacaagttca
     7681 tcgaacacac cgtgtcctac aacctcgtgc cgaacccgga cgtgttccgc gaagccggca
     7741 gcgcattcgg cgacttccag aacttcctgt ccgaattcga cgccagccag ctgaccgaaa
     7801 ccatcgccca cttccacgac accccgcatc gtttcgagga cttcaaggcc gccctcgccg
     7861 cggacaagct cggccgcgcc gccgcatgcc agccggaaat cgacttctat ctgagtcacg
     7921 ccgaccagta tgccgtcgtg atggatgggc tcagggacgg ttcgattccg ctgcgcgtga
     7981 cccacaatga caccaagctc aacaacatcc tcatggacgc caccaccggc aaggcgcgtg
     8041 cgatcatcga tctcgacacc atcatgcccg gctccatgct gttcgacttc ggcgattcca
     8101 tacgctttgg tgcgtccact gctctggaag acgaaaagga cctcagcaag gtgcatttca
     8161 gcaccgagct gttccgcgcc tacacggaag gcttcgtggg cgaactacgc ggcagcatca
     8221 ccgcgcgcga ggccgaactg ctgccgttca gcggcaacct gctcaccatg gaatgcggca
     8281 tgcgctttct cgccgactac ttggaaggcg atatctactt tgccaccaag taccccgagc
     8341 ataatctggt gcgcacccgc acccagatca aactcgtgca ggagatggag cagaaggcca
     8401 gcgaaacccg cgccatcgta gccgacatca tggaggctgc caggtgagcc gtcagccgaa
     8461 ccatacccga accagcatga tggaggcaga gcaatgaacg atcagctgac cgaggtatac
     8521 gccagcatcg acgccctgat cgattatgcc ttggcccatc ttgaccttga cccacgcaac
     8581 gcagactgga cccgcaacca aatcttcgcc ctcttccgcc tagattccta ccccggaccc
     8641 aaaaccacaa cgtcggcggc gtcagtgtca gacgtagtcc aagacattgt tggcagccga
     8701 agccaggccc cctatggcga aaagaccccc gaccccctgc tcgccgcctt ccgcgccgcc
     8761 gccacaaccg ccggcctttt caagccggag gaaggcccgg cctacgccga caccatcatg
     8821 ggcatcctct ccgccaaccc cgctgatctc gacgaccgat tcctgctcgt cgagcaccgg
     8881 gatggcggca tggccgccat gcaatggttc tacgactact gtgtggccaa caattatgtc
     8941 aagcgtgccc agctcgaccg gaacccgcgc ttcgactcgc acgggctgac ggtgaccatc
     9001 aacctcgcca aacccgaatt caagaacatg aagaaggcgg cggccggcaa cgccgtggcc
     9061 ggcggctacc cgaagtgcac gatctgccat gagaacgagg gcttcgccgg acgtgacaag
     9121 cgcacgctgc gcaccttgcc ggtcacgctc ggcggcgagt catggttctg gcagttctcg
     9181 ccctacggct acttcgacca gcacggcatc tgcgtgaaca ccgaccacac gccgatgcat
     9241 gtggaccgcg acaccttcgg ccatctgctc gacttcgtgg accgtttccc cggatacttc
     9301 ctcggatgca acgccgcgct gcctcgcatc ggcggctcgg tgctcgccca tgaccattac
     9361 cagggcggcg gcgaactgct gccgatgcac aaggccgcca cttgggccgc cttcacactt
     9421 gccgactatc cggacgccgt ggttgaaatc cttgactggc ccggcaccgc cgtgcgcgtg
     9481 gtgtcgaaga gtcgccagag catcatcgac gtatccgaca tcatccgcga ggcatgggtc
     9541 ggttatgacg acgcggcgaa cggcatcgcc agccatgacg cggatggcaa ccgccaatcc
     9601 gccctctcgc ccagcgcgat catcaccgaa cgcggctatg agatgagtct catcttccgc
     9661 aacaatgcga tcagcgacga gtacccggaa ggtattttcc atgcacaccc cgaatactgg
     9721 ccggtcaagc aggagccgat cggactgatc gaggcccaag gcttgttcat tctgcccgga
     9781 cggctcgtcg accagctcgg cattgtcgaa gaggcgctcg ccgaaggccg cgatttgccc
     9841 gatgaagtca gcgaattcag tctcgaatgg ggcgaactcg ctgaaacgtt ggccggaaac
     9901 cacgaccgcg aggccatccg ccaagccgta cacgacgagc tcggcagcgt ctgctaccgc
     9961 atcctcggca acacggccgt cttcaagcag aaagccacca cgcaaacatt cctcgaaagc
    10021 cttggttttg ccgctaggtg aaacaggtca agtgaagtaa gctcaatact gaaggaaaaa
    10081 ccgaaggcaa gtccttccaa aggagggaat atgactactg ttctggttac gggcggtgct
    10141 ggattcatcg ccacccacac cgacatcgaa ctgctcaaca agggctacga cgttatttcc
    10201 gtagataact acggcaactc gtcgcccgtg gcgcttgagc gcgtcgagca aatcaccggc
    10261 aagccggtca agcgttatga cggcgacgtg cgcgatgagg cgctcatgnn nnnnnnnnct
    10321 gaaaacaaca tcgactgggt catccacttc gccggtctca aggccgtggg cgagtccgtg
    10381 gccaagccga tcgaatacta cgacaacaac ctgtactcca cgcttgtgct gctcaaggtg
    10441 atgaagaagc acaacgtcaa gaagatcatc ttctcatcct ccgccaccgt gtacggcacg
    10501 ccgaaggaac tgccgatcac cgaggagacg ccgaccggcg gcaccaccaa cccgtacggc
    10561 acctccaagc tgttccagga gcagattctg cgcgacgtgc atgtggccga tccgtcctgg
    10621 accatcgtgc tgctgcgcta cttcaacccg gtcggcgcgc acgagtccgg cctgctgggc
    10681 gaagacccga agggtattcc ggcgaacctc accccgtacg tggccaaggt cgcggtcggc
    10741 gagctcaagg aagtccaggt ctacggcgac gactacgaca cgcccgacgg cactggtgtg
    10801 cgtgactaca tccacgtggt cgacctggcc aagggccacg tggccgtcat tgaccacatc
    10861 gacaaggaag gcgtgttcgt ctacaacctg ggtactggcc acggctactc cgtgcttgag
    10921 gttatcaagg cttacgagaa ggctgccggt catccgattc cgtacgcgat caagccgcgt
    10981 cgccccggtg acatcgccgc ctgctacgcc gacgcttcca aggcggagaa ggagcttggc
    11041 tggaaggccg agctgaccat cgacgacatg gccgcctcct ccctcaactg gcagaccaag
    11101 aaccccaacg gtttccgcga cgcggagtga tatgagcaga tgagctccct ctgatgaggg
    11161 agctcatctg tcgttcatca cacgatgcgc ctatcggcgg cccagcgggt gagnnnnnnn
    11221 nnngctcggt gcgattcgaa agctgaagct ttcgcagcac cgagctcacg tgcgtttcga
    11281 ccgtcttgat cgaaatgaac agttcggccg ccacctcttt atatgtgtaa ccacgcgcga
    11341 tcagccgcat gacctcctgc tcgcgggcgg acagccggtc cagctcctca tcatgagccc
    11401 gtgccgcacc atcagcgccg ccatcagacc ccgccgccgg cacaccctgg aaggtcgaaa
    11461 gtacaaaacc ggccagtttt ggcgagaaca cggcatagcc ctcatgcacc tgcttgatgg
    11521 atgaaatcaa atcagcgccg gtaatcgtct tggttacata gccctgggcg ccggcgcgaa
    11581 tcaccgaccc cacatcctgc ggcgcatccg aaaccgacaa tgccagaaac acggtagtgg
    11641 gggagtaggg ccgcgatttg gcgaggatct cggcaccgcc gccaccctcg ccgccgggca
    11701 catgcacatc cagcaacacc acctgcggct tggtctgtgc gatcatcgcc accgaaccgg
    11761 gcacgtccgc cgcttggccg acgatatcga aggaatcgcg caacgtggcg attacgcccg
    11821 cacggaacat ctcatggtca tcgaccatgg ccacacgaat cttggctgtc tcgttctcgc
    11881 tcatcgatgt tctccctgcg ccgctttcag cgcgatcggc atatgcattc tgacctcggt
    11941 gccccagccg gccctggaca ctatctccac tgtaccgccg cgccgtttga tgcggccgat
    12001 aatcgattcg cggataccca gccggtcggg cggaatggcg tccatatcga agccttcgcc
    12061 atggtcgcgc acgaacactt caaccatcgt gtcgctggcc tcgcaataca aagacaccgg
    12121 ctcgccgcca tgcgtgaccg cattgaccaa cgcctgacgg gtggcgtcca gcagcgcatc
    12181 ggtctgggcg ctcggccgcg cgtcgcccac ggtgaccacc tcaatcggct tgccgtgctc
    12241 gtcctccacc tcggcggcga tctgcttcaa tccggctccc accgaacgat ccgatgtgga
    12301 acgttcctga tacagccatt cgcgcagctc gcgctcttgg ctacgcgcca gggcgaatac
    12361 ggtggtcggt tcctcggagt tcagctgaat cagcgccaac gtttgcagca cgccatcatg
    12421 cagatgggcg gtcatgtccg cgcgctcctc ttcgcgctcc ttcaatgcct gctcgtgggt
    12481 gagtcgttgc agcatcgcgt tcgcccacgg catcaccgcc accacgatac cgacgatcat
    12541 cacgaatccg ctggcaacaa tgcgtcgagg agaggtgccc cacccaggaa tgctgacatt
    12601 atgcacatac aatgcccatc ccatgaaaat cagtgcgatg ccgcccaaca tagacaacag
    12661 ctgcgtgcct cgcggactaa gattcatcca tgccagtgcg atgccgccca accctaccag
    12721 cagcggaaac acaatctgga catccgcacc ggtgctggtg agcaacagtc cagtggccat
    12781 caacgcgaaa ccggccaatg ccacgacggc cggtttcgga gcacgttgga tggcctcgga
    12841 caatgattcc ggtgcggcag ccgattcgtc gtcatgagcg gcaagtggct gattgcctcg
    12901 cgcaagcggc gcctgattaa cgggcactgt gcgagccgca gccgccaccg ggtctccggc
    12961 aggcatgaac acccacagga atacataagc gatgatgcct gcgccgtaga ggacggtcag
    13021 tgcgatgaac gcaatccgaa cccatgccac gccgatgccc aagtgcaggg cgatgccctt
    13081 gcacacgccg gtgagccaac ggccctgccg ggggcgcatc aacggcatgc gcgcgggcag
    13141 caatggttcg ggagatgacg tcatactcct attgtgccgc gctcagggcg ttgcggccgg
    13201 ggttttaccc ggtgttcagg gaacaatcag ggtgttcccc gagcgttgtg gcagtggtcg
    13261 ggcggtgtaa tggaatcatg agcaatccaa acgcgaatcc atatcagcca cagccgcagc
    13321 ctggtccgca aaaaaacaag acatcgcgac gcttcttcac ttgggtgcga tccagtggac
    13381 tggttcgcgg cgacgaccgg tggatcggcg gcgtatgcag cggcatcgct ttacggcttg
    13441 gctggagccc cacattagta cgtgcgttgg tgattgtatg tacattgttc ttcggttttg
    13501 gcgcagctct gtacgcactc ggctggtttc tgatgccgga tgtacgcgat gggcacatct
    13561 tggccgagga cctcattgcc ggccaatggg actggaattg tctgggatgc ttcctgttcc
    13621 tcgccgtggc aatcgtgatt ccaggtgcgg ggtggggttg tattgcgctc gccgccttag
    13681 cgctatggct gttggccaaa agcggcattc gccagcagga agggtacgga ttcggggcat
    13741 ctgcgccgcg atcggtttca cagcctgtgt catatccagc tccacagcct gtctcatatc
    13801 cagccgcgca accagtacct caatctcctt tgtattcagc tccgcaacct tttccacaac
    13861 ctgtttcaca acctgtttca caaaccgcgc ctagtgtttc acagcctgtt tcacagcctg
    13921 tctatcagtc gatgcccagc gtgatgccgg gtactgtgcc cggcgcgatg cccggcacag
    13981 tacccaacgg gccgtttgcc cccgaccgta tgaatgcgac ggcctatgcc cccaccgagt
    14041 ccaagcgccg caagccggcg ggcccaatcg tggtcttgag catactgggc cttaccttcc
    14101 tctcctttgc cggtctgatg ggactgattt gggtaaacga catgggcatt atcggcatta
    14161 tgcgtctggg caccatctgg attgccgcag tatgcatagt gatggggctg gtggtcatca
    14221 ttctcggatt cagaggacgg cgtaccggtg gtctgattcc tctcggactg gttgccggtg
    14281 gatgcgcgct ctgcatgacc atcgtttcag gcacgtatgg cgtgtactac cgtgatcttg
    14341 gtgccagcgg catcaatact gttgtttcac tgagccaatc tgtcagtgcg agaaacagcg
    14401 acggcatgaa cgatgtgcaa aaggatggca tcttcgttgc cgatgccagc gatgccacgt
    14461 ttgaaacatt gcagcaaggt gtggcgttca gcggtgaaaa ctacaacacc gatcaggcaa
    14521 tcattgattg gtccgattgg gacaagacac atggaccgca cacggtgaag atgctggatg
    14581 gcaaaacttc catcagtaat tgcccggtcg gtaccatcac catagccgca acacaggcgc
    14641 aagtgcatat cgttctgccg gattcctgca cttacgcctt cggcaccgcc gactcctact
    14701 actccacttc gagtgacgtt ggcggcaaat acgagatggt atacaacaac tacatgggtc
    14761 caagctttgt tttcttcgaa gctgaaagtg ccaccaatgg gcctgatcac gaatggttgt
    14821 ataaggaatc cgccatgccg gacaacggcc cagaattaaa aatcaatatt ccctatgtgg
    14881 tggaagcacg tgtcaacgtg gtgtacgcct ccgattggga aggctctacg ttccagccgc
    14941 ttgccgatat atacgacggc aacagtacaa gcaagaacac aaccgaacaa tcggaatgat
    15001 caagccgttg caactaggag atggaatcat gactgatgaa accaaggaat tcgcctccat
    15061 tgaggatgcg acaccgacca cggccatacc ttccgaaacc gacgcgacca ccgcgaccac
    15121 aaccatggtg accgtcgatg accgaaccga taccacggtg ctgccatccg cagcggatga
    15181 tgacgaaacg gctgtgctgc ccgtccccga caatcatgcg gatgcgtcgg tctcggatgt
    15241 ggatacgcnn nnnnnnnncc ctggaaacag ttgccgatgc cgtggaggga gatggtgggc
    15301 cgattccatc tgccgatgat gtgccgatgt acgcagcatc ttcagcacga cctccctatg
    15361 ggcagtctca gaccgccggc aaaccatgcc agcctggggg gagcgccaag aacgtccaga
    15421 gcgccaagaa cgtccagaat gctaaggatg tcaagagtgc caagaacatc cagaaacaga
    15481 gcaccagcgc gcctactgtg gtattcggac tattaggcgt gattatcggc gtgatcgcgc
    15541 tggttttcgg gtacatatct ccggatcgga tgatttcgtt gttctcgccg aatccgcaat
    15601 tgctgacggc tgttatttgc gccgtagtgg gactgattct ggtaatagtg gccgttgtat
    15661 gggccatagt cggtgccgtc aagaagagaa aatagcgtta caaaccattg cattacggtc
    15721 ccagtagaag cacattatga cgggagccga ccgataagag ccgaccgttc tatgctgatg
    15781 gggggaggca tagtaactat gaagcggatt tcggtgcggc gggtagcgag cgtcatcgtc
    15841 accgcgatgg cgatgacgct ggtattcgcc acacttggct gggcgatgat gccgcaatgg
    15901 aacacgcggc cctacaccaa gcacatcgtt atcgcttctg ccgacaccac catcacccct
    15961 gcccatgcga acattcccca cgaggggcga tatcgtacgc gtgaaacccg actatccatc
    16021 aagaccggcg acggcgtcac gctacccgcc gtactacgtg aaccggtcgg cgctcccggg
    16081 ccacgcccgg cttgtctgtt cattcacggg tccggtacca gtggtgccga ggatttcggc
    16141 gatatcgcca acgccatggc ctctgccggt atcgtgacct tggtgccggc caagcgcaac
    16201 gacaactaca ccgtgctgca tcgcgactac ccgcgttttg ctcgcgaata cgggcgttcg
    16261 ctcgacgtgt tgcgtggcag aatcggcgtg gaccccgcca aaaccggcat ctacgccgaa
    16321 tccgaaggca cctggatcgc cacgattcta acctcgaaac ggcaagacat tgcctttgcc
    16381 gtacttacat ccgcaccggt gttcaatgga cgtgagcaaa tggccatggc tgtcagcgcc
    16441 tatacgcatg aggccggcgc gcccaagccc gtggtcaaag acatggccaa attgatgtcg
    16501 ctggactacg cgccatttga tctagcctat gccgactttg atgccgaccg gtatctgaaa
    16561 tccctcacca tgccggtgct ggtgaactac ggcacctacg atacggccat gcccatcgaa
    16621 cagggggcgc agcggatcat cgctacggcc aacaagtccg gcaacgagaa cgtcaccgta
    16681 cgctattttg ccggcaacca tcagatgcgg gctggtgagg gcttattcac cccgaacctg
    16741 ccattggccg aaggctatac gcaagcgctg gaaaattggg tgaacggtgt gactgccgga
    16801 accaaagccg atggatgggc aacgcctcaa gtcgccggtg caacacctca ccagcgcttc
    16861 gccgcgtcgc aacgtaccag atccggtatc gtcggctcgc ttggcgtgct ggccggtctg
    16921 atggtggcag ggcccgtgct gatcgtgatg gcggcgatat taggtattgg cttgactgtg
    16981 ttttcttggc tacagacgtt gctggccggg cgacgtagtg tggcgactgt gcgggccatg
    17041 catgcgacgc cgagtgagct gggggccgcc cagcggcgca tgttgcacgg cattgccggc
    17101 ttatctgccg gtatcggcac agcggtgatg gtgattactg ggctgttgta cggctatatg
    17161 agcgccgtag gcgtatcggc tgtattggtt atgcctcaac cgcggctgtt tgccgtcgga
    17221 tgggtggtgc tgcgaatagc gaccatgctg ttggtggtgc tgtttgcttg ggaaatggaa
    17281 cgtgtctggt attgccgcgc cgatatcgtc ggtgtgcggc gcgtcatatg cgtgatggta
    17341 gcgctcggca ctctcgccac actgatgaca ctggcatttt ggggcctatt ctcgctgtaa
    17401 ttctgccgat gtgcgctggt aaatggggcc tggcgggcag gctgcgttgg tggtctgggt
    17461 gtggggcgat cggcagattg cgttggcctg ggtatggccg gccgacgtac atcagtcggg
    17521 gtgtccgata cacagtgaat ggctgagtat tacgcttggg aggcttcggc ctcctctaag
    17581 gtgtcgaaac cgccgagctg gactgtgtgc aacaaggttt cctcagtgat attgcgttgc
    17641 gggctggctg cggcgcgctc gccaatcatc gcttcgtaac gttcgaaggt ctggtcggaa
    17701 tacgtaccaa gctcgccgcg cagataggtt tcgaatgagg tggcagatgg tgtgtcttcg
    17761 gtggtggtga gtgcgcgcat cgcctcgccg agcttggggt agcgctcgcg gaaatccttg
    17821 gcccaggcga cctgctgggc gatgacctgt tcctgacggg ccgcgcgctc ttcagagaga
    17881 cgcggaatat acggctctat gttcttcgtg aaattttccg gtgcggtgga ggccatcatg
    17941 cggccgtatt tttcggtcac gaggttgcgg cccacgtggt cggccgcgtc aagatcagcg
    18001 gcatagctgg tgagtaatgg ccgctcccat gtgaggaact gggccaaccg catctggtgg
    18061 aacaccggcc agttgccttg gcatgccgcg cgtccaccct cattgttggt gcgctggaac
    18121 tgatcccatt catgacgcac aatagcctcg cgcagttcat catcagtcag ttcgttggtc
    18181 atcgacgttc ctttcactga tacctccctc tgatgaggga ggtggcacgg cgaagccgtg
    18241 gcagagggag cttgaatcta caaactttta agcaccggat catcagaggc gatgtggtcc
    18301 tcgacatacg ggcgctgcca ttcgaggaat gtctcgccgg acgtggtcag gtgttcgcgt
    18361 ctgagttcct tgacgatatc caccgcaatc tgctccacaa tatcgttgat tttctcgatt
    18421 gctggagccg cgcccttgcc gccttcgcca aatccggccc cgccatagca ggctgccgaa
    18481 ctcaatcgca tcacggtttc caactgttcg cccacgttcg gcaacagcgc gaacatcgag
    18541 ccagacagct tgcgcaatgc tgcgaactgc cacttgtaat acggcatata gcccaccacc
    18601 atcggcacgt taaccaggaa caccaacgag gcggtggcct gcacgaactc gtggatggac
    18661 agccatgcgg ccgcgccgtc gccgcgcttc aacgaacgcg gcagattata ttgaccggcc
    18721 tgggcgatca tgccgagtcg cttggagatc agcgcgagcc gcacatcgtc gggcatgttc
    18781 ttgaaccctt ggcgcgtctt ggaaaacaga ccggtgggat cggcgaacac ctcgccattg
    18841 gtggcggcgg ccaaggtcga ttcctgaagc attagccact catgcggcgc ggtctgcgcc
    18901 ggcgcagtgt gatacccggt gatcgactca aagaagtcac cgatgcggaa cacgccgtca
    18961 cggcgattcg cgccctgtgc gcgcggcgtg acgggggaga gcggtgcatc aatcggagtc
    19021 gtcatcgctt ccgccacatc agaggacgcc gcgcctgact cggcagtggc ggtatcgtgc
    19081 gtagcggtgc cgggggtggg tgcgtttgcc gcatcgggta tgacggtgga gcctgcacct
    19141 gcggagggga atgctccgga agcatcggac ctggcatgcg cctcgaacgt cacgcggcct
    19201 tgcgcatcca cgctgaactt gcgcggcagt gcctcgtaat cgacctcaag ttgctcgccg
    19261 atcgccgcgt aatcctcatc cgtaagccac aggcagaagc gcgggccgaa atcgtggtcc
    19321 tgcgaatacg catcatcgaa tccgtagcat tccgagccat gacccaccag accggcggcg
    19381 atacgcccgg catactgcgg atacttcgcc gcaatcatcg gcttgcccat ctgcgtccag
    19441 aacgcgcggg ccagcttcag tccggacacc gggcatgcgg aaacggcctc ggccgcgccc
    19501 gcagaaccgg tcgacaccga aacggtggat ggggaagatg ccgcgttcgc accggtcttg
    19561 ccctgtgccg ggctgttcgg cagccgcgag ccggactggg gcagcgcgcc tgcatcgccg
    19621 gcaacacctg cattgtcaac cgtcaccccg gcctgcgccg ccgcttcctc ggcctgccgc
    19681 agattgtcgg cggtgatacg ccagtaatcg gtgtccttgc cgtaacattc ctcgatcacg
    19741 gccagcgcat gccggtatcc agtaaccgca tccgcatacc gtctctcggc aaagcacacc
    19801 tgcgcgaagc ccgccagtgc ggaggcataa tgcgcggaat gttccagatg gccggtgcga
    19861 tagatttcca gcgccttggc tgcatgccaa tgcgcgtcct ccaacttgtg ttcggtcagc
    19921 agggtcaacg ccagattcgt gtgcgagctg gacacgtcga tatccgcgtc cgggttcacg
    19981 ctcgaggctt cgataatgcg cagcgcttca cgcaactcca gttccgcctt gtcgggccga
    20041 ttcgtctcgc tgtagagcat cgacagattg ttgtgcagtg ctgcgagcct acggtcggtg
    20101 ggcgccagcg aatgccggca gacactctgc gcctgatgat acaaatcctc ggcctgatcg
    20161 tattgtttgg ccgcgcgcat cgaggtggcg caattgatta gcgtgatggc ccacgcctcg
    20221 gatgtgccgg tggtcaagcc catacgagcg gccagttcca gcgctcgctg cacaatccac
    20281 tgattctcct tgtgccgacc ttgcgagcga tagaagccca tcgtttcgtt gagcacggtg
    20341 agcagtccgg cctcgtcgcc ggcgttttcc gcgtccacca tcgcctgttc caagtacggg
    20401 ccggcttcgg tggcggccgt gtggcgatcg aaaatcgcgt ccagtccgtt caggaattgt
    20461 tgggtatcaa aggttgcggg cgattgatcg gcatcgtccg caacctggtt cgccgtggaa
    20521 tcggcacctt gtgctgcggc cgccgcatcg gtgtcgtcgg catcgtctgg accatgcgcg
    20581 aattgcgcgg cgaattgttt gagcagtgct tcctcatcca taccgccaga cctccctgaa
    20641 tcgtgtctga tttcagtcta ttaatactgt gaacaaacac gtcagggagt ttgctgtgag
    20701 cttggtctca gtccaccttg gtttcgcgca agcggtgaat ggcgtcggta tcggtttcgc
    20761 agcgccacga gtagaaggcc agcagcccta cggcaagcag agccagcggc ataccgccca
    20821 ggccggtata gcgatagttc atcgagaagg tggtgagcat cgcgccgccg acaatcgagc
    20881 cgatggcatt gccgaagtta aacgccacct gtacagccgc gccggcgatc agctcgccgc
    20941 cgccctggcc ggcttcggtc atcagcaatt gctgtggagc ggaagtaaag aacaatccga
    21001 tggcgatcca gaacgtcagt acagcggtca tcggctgatt acccggagtg ataagcacca
    21061 gcaacaagcc gaggcaggag atggactgac ctattgcggc cgcgccggca tgcctccaat
    21121 ggtcggtcag acgacctcca agcagaccgc cgatgaccat accaaagccg gcaagcatca
    21181 tcagcatcgg caccagggaa gacgaccatc cgccggtttt ctgcagccaa ggggagacgt
    21241 agctccacca gcagaacaca ccggcattgc cggtgaacac ggcggccaaa atcacccacg
    21301 ggccacggcg ggtgaggaac ttgaactgac cggcgatgcc tgcgtccttg atcggtgcaa
    21361 cgtacggaat ccacgccgac gccagcacaa tcgtcattgc cgcccaagcg gccaaaatgc
    21421 cgaaggcaag acgccaggaa aggaactcgg ccatcaaggt gcccgccggt acgccgagca
    21481 tattggccac ggtctgtccg gtgaccatca cggatacggc ctgcgcctcc ttgccttcct
    21541 ccgccagagt cttggcgatg agtgtggctg tgccgaa
//
LOCUS       DAWBMW010000014        20277 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_715_length_20277_cov_3.473296, whole genome shotgun sequence.
ACCESSION   DAWBMW010000014 DAWBMW010000000
VERSION     DAWBMW010000014.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 20277)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 20277)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..20277
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_715_length_20277_cov_3.473296"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         4353 a         5770 c         6015 g         4139 t
ORIGIN      
        1 aacactcacg acccaaaccc acacaccccc acaaaaacac ccggggcttc tcgcactcat
       61 gagacccacg tccgggacca ttcccggccg gccccgccgt cggggccgaa gagagacggc
      121 gcggcgcgtt cccatttccg caggaaatcc ggaaaacagc acgtccgtga ccgtggaatc
      181 ccgacgcccg ggcctcctcc ccccggtctg ctgtgcggac ggctcccccc ctgaggggag
      241 ccagaaactg gcttggttat ggggtcgctc tacactggct tctgctatgc attacttcac
      301 ccctaaacgt tgcgctacgt cgaataaaat catgtatcac gataaggcgc aggttcagcg
      361 tgccgctgat caaagcctaa tcgagcgcgg agttgagcta tgggtgtacc gttgcgaatt
      421 ttgcggatgc tggcatctca cgcatcgcga tccgcaatcc agttaccggc atgtaccgct
      481 gaaccagcaa ctcaagccac acagtcgtaa aaagggctac aagccacgta gacgttagcg
      541 tcagaagctc cacaggcctc gccaacgccg tgtctgagac aggttacggt ccaaacacgt
      601 caggtcgtag gctgctaagc gttcagggcg ctcggacaca cttcgtccaa cacttacatc
      661 accttggtgt tttcaaggtt gcggataacc caatcgttga agcgaattac cggcttgcgc
      721 aacataagtc ccaacagcaa tgaggggaca aggaacagtg ccagtttgcc catggaaacc
      781 cagtattcca tgccgaatga gcctgccatg gccgaatgca tcgcatccac cgcatagggg
      841 aacagtagcc atttagaaac gacctggaag aacgtcggca gtgtttcaat ggggaatgtg
      901 ccgccggaac cggctacctg cataaccagc agtactacgg caatggcttt gccgatatca
      961 ccgaacgaca gggtcagcgt atacacaata ttcgagaaca ccagtgcaga caaccacccg
     1021 accatcaaga actgaatcgg atgctcacac tgcacgccga gatagaacat atcgcccagg
     1081 cacaccagcg taccttgcag caaagcaagg aaaccgaaaa tcatgtatcg gccgaaatat
     1141 tcgtggtgca agcgtaaacc gaactgccgc gcattgccgg gagcctcggc acgcggcttc
     1201 ttcagcacat ccaacgcggc ggccggtccg gcaatccgcc cggcaatcat ggcctcgcga
     1261 agacccactt ccgaaccgag cggcagatca ttgcccaacc ctaagatacg agccttctca
     1321 tggtcggaaa tcgatacttt caacatggcg accagaatga tcgcaccgac ccaaatagac
     1381 aaaatcgtgt agaacggagc catcgcggaa ccatagttgg caacgggata gagcgcgatg
     1441 cggtcgaccg ctacgggtgc cgccagcatg gaagcaatgg catcgggctt cgcagaggaa
     1501 atcacagaca ggtcgatggt gccgccattg gtgatagagg cgagttggga agacaagttg
     1561 tcgagtttct tcgccgaacg ggtcaaagcg ctagctgcgt cagtcaaggc cgactggatg
     1621 tccgtcaggc tgtctgaaac gccgccagaa agacctgaca cgctgcttac ggtcgatgca
     1681 agcccgtcga ttacggaact tgcctgcgtg gtcacttcgt ccatggacga ggtcagattg
     1741 ttcagttgcg gtttcagcgt gctcttgtac gtgtctgaag cctgagtaat cgattgtttg
     1801 gcggcagcga tttgtgattt cagatctcga cgcgctgtat cggcatcggc agtaccgttt
     1861 gctatgtgcg tggccgcatc gcccaacgcg gtggcggctt gctgcatgct ctgctgcgcc
     1921 tttgtggctt ggtctgcagc cgcgttcagt tgagcggcaa cggtatctcc gcccacgggc
     1981 agtgaagccg cttgtttggc gagatctcgt aaagcggcgg catactttcc aaagctgtcg
     2041 gcctgagcct ccactttgga ttgcatatcg gttagcttgt ttgatatctg tgaggattgt
     2101 gagcccacat cggcaaaagc ggcatcgatt tgcttggcaa tggtgtcgta ggatgaaccg
     2161 gcctgctgca atgccgtacc gacgccatcg gtggctccgg acaatgcctt gtcgaggctg
     2221 gagacgccgt tgcgggtctg tttcaacgcc tttttggttg tggccgcagc tgagcctgtt
     2281 gaccccagca gcttatcggt ggaatcaatg atgctgttgg cggccccgag cagattggca
     2341 taggaatcca tctgcgacgc agtgccggtc aaacgagacg acatggtggc aatatgactg
     2401 gtggcgttgt tgacgtactg ctccatttgc gggctttgcg cgtaatcggc cacgttggaa
     2461 accaaatcca gcgccacgct cgccaaggtc ttcacgaatg tctggtcgat ggtcgtggcc
     2521 acggtggtag caccctggtc ggtgatatgc ggagcgatcg gattgatttt ctcattgaga
     2581 tagtattcga gcttggcgtg cttgatcttc ggtgaaaaca gcgtcatcat atcggagctg
     2641 aagctctttg gaataatcag cgcggcatag tattcgcctg agttcacgcc atcgatggcc
     2701 ttagcacgat ccacaaactg ccagtcaagg tcatgattgg ccctgagcgt attggtcacg
     2761 gtttcgccga cgttgatggt gatcggcatc agatcggact tatagccctt atccacgttc
     2821 gccacagcga ccttcaaggc cttggtgttg ccgtacgggt cccagctggc ggcgatgttg
     2881 taccaggcgt acagtgccgg cacaatcgcc aatcccatag ccacgattac ggcgatcaca
     2941 ttgcgggtgg cttggcgcac gtcacgtacg aatattttcc agataagctt catcgtgcgc
     3001 ctccctgccc aggctgcccg gactgaccgg actgtgcggt gtgcgcagtc ggctcggtgg
     3061 cctgatcact gaacggtgtg agtcgttcag tggtttcgcc gatattcgga ttgctatcca
     3121 tcgccgcttc gtgacgtccg tggccgtgga aacgccgcca cacatatccg ttgagcatca
     3181 cattgcggaa ctcgtcggca ctcatggcgc tgacgccaag ctgcatggcg taggcttcgc
     3241 gtgcgtattc aaccacaatg agataggcgt cgataagaat aatcgacaga atccaagagg
     3301 tcagcatggc gattttctct tgggtgacga acagcagcac caggaatacg aacgggagta
     3361 cggtcagcaa tgccagaccg gcaacgatca tctttggata ccgtgcgaag aaccggtgcg
     3421 cgcgatgggc gatgatatcg cgcagttcct tgctgtcgga caccgtgcgg aatacggagt
     3481 tcaagctcac ctgttcgttc accatgctgt tgtgctcggt gatcatcagg tcggtatctc
     3541 ccaaacgacg gtcgaacagc gcgttcaggt tcatcgcata ccggcgtatg acaagtccca
     3601 cgaagagtgc cgccggcaga taccagaaca ggtgcagcat gtcgagccag taatggttcg
     3661 cgtacaggcc ggcaatcggc ccgcgcatcg cgttgatgcc gtaggtaaag ggcagccacg
     3721 ggtgcagttc gcggaagaag ttcggcatca tttcaatcgg atacaggccg gaggcgccgg
     3781 gtatctgcac aatgaccaga attacggcga tggctttgcc gatatggcgg aacgccacgg
     3841 ccagtgcgta aataatgttg atgtatacga acgaacagaa gatcccggcc aagatgaaca
     3901 gcagcggatg ctcgcattgg atgccaagca ccagatcgcc gatggtggcg atgacggctt
     3961 gcaggaatcc gatggtgacc agcagcagcc agcggccaag atatgcttgc gtagcggtga
     4021 tacggcgaat gccctcgcgg tcgacctcaa gcttgtagat ggcgattagc acgaaaccgc
     4081 ctacccacag tgccagattc gtgtagaacg gggtgactgc ggacccgtaa ttgtcggtgg
     4141 catacacgac tttcgtggtc agggtgacgg gattgcccat gaagtcgccg aatcccttgt
     4201 catccagatg cagcgtatcc cggattttct ggtaagcggc ggaactatcg agcgtggcga
     4261 tatcggtgcg tacggtggcg acatcgctgg ccacctggcg cagcgaatcc tgcgtgctgc
     4321 tgatggtggt ttttgtttgg ttcagggtat cgacaagctg gtcgaacagc ccgtcggcct
     4381 gatccaaggt gccggacaac gaagtcaacg tgccatccag cgtgccgttc agggcggcga
     4441 aactgttcaa tgatgaggtg agcgacggca tcacggtgcc ggtcagcgag ccgcgcgcgg
     4501 tattcagcgc cgcgatgccg ccggtggtgg tgttggtcat ggctccggaa aggttcgtgg
     4561 tggcgtcttt gccggagttg atgaactgtg tggtgtcggt atggaaggtg ttgagctgtg
     4621 tctgctgttt gctgatcaat gagtcgagcg cgtcgatttg tttccagatg ttctggccga
     4681 ccgggtcggt ggtgtctttg ccgatgccgg ctttggtcag cgcgtctttc agatcgctga
     4741 ttgacgtttg ggtggcgttc agtggggccg aaagtgtgtt ggtgatggtg tcgacggagt
     4801 tctgcgcgct gttgaaggaa ccggtgatgc tgccggcggc gttgttgacg tggacggcca
     4861 gtccggagag ttgtgctgaa ctgctgtcga gcgcgccgac cagtgtggtg gagaaggctt
     4921 gtgacccggc ctgtgtttcc gccaacagtg tgccggactg gcgggaggca ttctgcgcgg
     4981 ccgcgacggt ctgcttgagt gaggcggtgg tctgtttggc gtcggcgaga atcgtgtctg
     5041 attgggccag cgtgtcctgt aagtcggtga gtgaatcctg aacggtgttc agcgtggaca
     5101 tcaccgtatt gagatcatcg atcgtatcgt gctggacttt gccggtgact tggtcgagat
     5161 cgtctcccgc ttcggaaacg gccgaggcaa cggtggtggc taccgtggtg acgaaggcgg
     5221 agttgatctg ctcatcgatg gtggtggcgc cggtgtcggt gattttcggc gcgatcgcgt
     5281 tcttcttctc gttgacgtaa tacttgattt tcggctgttt ggaggttccg gtcaccgatt
     5341 cgaccaagct ggcgctgaaa tccttgggca gcacgatggc tgcatagtat ccgccggtct
     5401 ccacgccgtg gacggcctgt tcctcattgt ctacgaagcg ccagccgagt tcgtcgttct
     5461 gcttgagttt cttgacgacc tgccggcccg catcgagatg gccgaccaga tcgttggtgg
     5521 tgccttggtc ttcattggct acggccactt gcagattgcc ggtggctgaa tagggatccc
     5581 agttggcgag aatgttgaac catgcgtaca gcgagggcag cattgctacg ccgagcgtca
     5641 ccacaacggc aacgggattg cgtaagatac gtctgcaatc gcgtagaaag acttgccaaa
     5701 ctgtccgcat ccagtccctc ttttccggtt cgtagtcatt gcggccacat gcagtctatt
     5761 cccaccactg gtgcgaatac tgctggtttg gcgggttttc ttgcaaaact ggccgttggt
     5821 tgtccgcata atgggactga tatgtgaacg tttgttgatg gtgcgacacc ggcgggatgg
     5881 ttgtgcaatt ttgccggtat ccggtgatga aatgttgagg tgttggaact gagtcgtcgc
     5941 agtgctgaga ccgagacgac tcagtgacga gcctatccga ggctatctcg ttggttctgg
     6001 tctcactgat ccagacggaa gccctgagcg gcggccttgt cgagcagtgt ctgatggctg
     6061 acatcctgcc gactgatcgt ggcgacctga tcgctgaaag cgtccaccgg acggtcggct
     6121 tggcgcagat cgtagtaccg gtgaacttcc tcgtcgaagg cgggcagctg gtcgagtacg
     6181 ccatcggcgt cagccgggta ggtgttctcg aagaactgca tcgtgcgcgg catgcgcggc
     6241 ttgacggtcg ggttctgatc gggcttgccg atggccagac ccagcaccgg atacgtgtat
     6301 tccggcagat tcagcacgtc gataaggccg gggatgttgt tgagcagaga gccgagaatc
     6361 acgcagccca gtcccagcga ataggcggcg gtctccatgg cgtgcagggc cagcactgcg
     6421 tcgttctgag cttgggtgaa gcgatagctg tacttgagat ggaattcgtc ggaagcggga
     6481 tcgataccct tgcgttcggc gatgcgtgcg ttgcggtgct cgtcgatgac gaacacgtac
     6541 agcaacggcg cggtggcgat atacggctga tggccgaatt cggcgagctt ggctttggtg
     6601 gccgggtcgg tgatacggat ggcggaccag tcgttaagga actggctgga cgcggcatgt
     6661 tgggcgacgg tttcgagcgt ggcgcgggta tcgtcgtcga tggcctcgtc cttgaactta
     6721 cggatggagc ggcggcccag aagagcctcg attgttgcgt tggtaactat ggctttattg
     6781 tcgcacggca gacgccggac gtcacggtgg attacctcac agtgaaatgc cgatattgtg
     6841 tggcggaact gtgctggact gatggttcct gtcgattgac tgatggttcc ttgacgaaat
     6901 tccagcgatg agtcatcagt cagccaacag aaaccatcat ccaaccgggg gattagcggc
     6961 tgggcttgcg atggcggtgg tcgacgaacg ccttggagcc ccgatccgac agctgcgaaa
     7021 ccactgcgcc gagtgcggcc gaaacggcgg cgaatgccag actcattgcc agactttgcg
     7081 gcgcgtctgc cgccagtccc ttgcgcagat tgcgtttgct tgtgcccttg tcccatatca
     7141 tctggaatgc tttgccggca accaagcctg ccagcgctgg gactgccgac ttgatgagct
     7201 tgtcgcccag agaatcaggg tcgttgagcc ggtcctcgcg cagcctgttg accttctcgt
     7261 cgatgcggtg caggctgtcg accgcgcggt cggcgctgga ggggaagtcg gtgttcgttt
     7321 cgtcgctcat gactctcagt ctaatagcat ctcaatcatc tcagcgcaaa agatgggcgt
     7381 taatggtgga gtcatgaacg agcgcagact agagtggagg catgagcgaa cagaacgtga
     7441 acggcaccgt tgccgtcgag aactctcgtg gcggcaatgg atttttggtg ttgctgcgcc
     7501 acggccagac cgtgtggagc gaatccgggc agcataccgg gcgcacggac atcccgttga
     7561 ccgcggtcgg cgagcagcag gcagcggatg ccggaaggcg cctgcgcgag gccttccccg
     7621 aaggattcag ccagagctgt gtgttttcca gtccgctgaa gcgggcgtgc caaaccgctg
     7681 aattggctgg ttttgcggac cacggtgtgt tggacggcat cgctgaatgg gattacggcc
     7741 gagccgaggg gcgtacacgc cagcaggtga gtgaggccag cggttttgaa tgggatgtgt
     7801 ggcgtgacgg gccgcgctcg ctgacgccca cgctggaggg cgactgggtc gagaccttgc
     7861 cttcgggcga acaggtgccg gtccatgccg ggcctggcga gacgctggaa gaagcggcgg
     7921 cacgcgccaa gagcgccatc gacgagatta cgccgctgct gaaagacggc cataatgtgc
     7981 tgttggtggc tcatgcgcac atcctgcgca tcctcacctc gcaatggctg ggcgtggacc
     8041 cgcatttcgc acggttgctg cgtttggata cggcgcacta ctcggtgctc agcgtctata
     8101 agggagataa cgtcatcgaa cggtggaacg cctagccccg gttgcgctgc gattgcgctg
     8161 gcctgcccgc tactgcgggc gggcctttat tgtttgtggc gggaattgcg gtatgtcgat
     8221 aaatgatttg caaacgatag cgtcggcgtg ttgttttttt ttgatgggta taagtggcaa
     8281 tcgattgcca tgaaagatat gttagcgctc tcatgattgc gaacgcttgc attgcgataa
     8341 tgaggcaaaa atgactgaat taatgggata tttgttgaca atggcctgaa aatgatgatt
     8401 tatgacgcgc tgatattgct atcgtttgca aaaagaagca aaccgttgca caatagtgca
     8461 tgtcgatagt cgtaaaggtg cgtgctagcg tacctgtcct gaccggggac ggccgggtcg
     8521 acgatgaaag agaaaggttc gatgatgaat cgtacagtca agtcagcagt cgcgatggcg
     8581 gctgtcgctg cgatgtccct cggcgctttg gcggcctgcg gaagctcgac ctccggcgat
     8641 gacgccaagg gcaaggtcta ctacctgaat ttcaagccgg aagcggctga tcagtggacc
     8701 gctctggcca aggaatacac caaggaaaag ggcgttgagg tcaaggtgca gaccgcggcc
     8761 tccggtacct acgagcagac cctgaagtcc gaaatcgcca aaaccgaagc cccgaccttg
     8821 ttccaggtca acggcccggt cggctaccag aactggaaga agtacaccgc cgatatgtcc
     8881 aatacggatg tgtacaagga gctcgccaac caggatgtgg ccctgaagga tggcgacaag
     8941 gtggtcggcg tgccgtacgt gatggaaacc tacggcctga tctacaacaa ggacatcctc
     9001 aacaagtact tcgcgctgga tggcgccaag gccacgtcca tggacgaaat cgacaacttc
     9061 gacaccctga aggccgtggc cgacgacatg caggcccgca aggacgagct cggcatcaag
     9121 ggcgcgttca cctccgccgg cttcgactcc agctccgact ggcgcttcaa gacccacctg
     9181 gccaatctgc cgctttacta cgagttcaag gatgacaacg tcaccgagca gccggccaag
     9241 atcaagggca cctacctgcc gaactacaag aagatcttcg acctgtacat cgccgactcc
     9301 accaccgacc ccacccagct gagcgccaag accggagatg atgcgaactc cgagttcgcg
     9361 ctcggcgagg ctgccttcta tcagaacggc acctgggctt ggaccgacct gcagaaggcc
     9421 ggcatgaagg ccgaatccgt gggcatgatg ccgatctaca ccggtgtcaa gggtgaagag
     9481 aagcagggct tggccactgg ttccgagaac tactggtgca tcaacgacaa ggcctccgac
     9541 gccgacaaga aggccaccga ggacttcctc tcctgggtga tcacctccga caccggcaag
     9601 aaggccatct ctcaggacat gggcttcacc accccgttca agaccttcga tgatgtcaag
     9661 tttgacaacc cgctgactga agccgctgtt gaggatcaga agtccggcaa gacccaggtt
     9721 tcttggaact tcaccatgat gccgtccgaa gagtggaaga acaaggtcgg ccaggcactg
     9781 ctcgagtacg cgcagggcac cggcaagtgg gatgccgtca agaccgcctt cgtggacggt
     9841 tgggcttccg aatacgaggc ttcgcactga gtctcataga gtcccatagc cgataagtaa
     9901 caatccgcgg cgcccctgcc atttccttct ccgggggcgc cgctttgtat tgctgagtct
     9961 cgcacaaaat cgtccgccgc aaaaaagtgt gaggcacgca cccgctcaat cacacactgt
    10021 gtgtccatag attggcgatt tttcgccatt tcccacaatg tgcgattgag cgttattgtg
    10081 cggttgcctc acactttttc tatggtgggc tttgttgtgt gagcctactt aacgcgggag
    10141 gtggaggaac ggaccttcag ctgggccggg aacacgatgt agggttgctc aatcggcttt
    10201 tcgtcaatga gatcgagcgt catgcgggct gccacgcggg ccatttccac cggatcctgg
    10261 cggatggtgg tcaggccgat atcgccggtg tagaaactat cgtcgtagcc gatcaacgag
    10321 acgtcgccgg gcacggagaa tccattgcgc tccagctgga acataagcgg caaagcaatg
    10381 ccatcctctt ggcatgcgat ggcggtgggc atctcgtctt ggcttatgag ttggttgacc
    10441 acgtcgctga tttcataacg tccatcgtca tcgaccttgc agacgataac ctgcggctcg
    10501 atgccttccc tgcggcagca ggcggtgaat gagtcgaatc ggctttgcac ggagaagctc
    10561 agcgacacct cgcgattggt gctgatgtag gtgatgcggc gatgtccgag attgataaga
    10621 tggcgggcgg ccagcgtgga gccctgcaca tcgtcgatat tgacggcggc ggtaaagccg
    10681 cgggcttcgg gttcgactga attgatgccg acgatcggca cgccgaccga agccagttgc
    10741 gcgatttcgt tgttgtcgat atcgaaagac gccacaatca cggcatcggc gttacggcgt
    10801 accggcaaca tctcgaagaa ttccttgcgc tcctcgatgc tggaaatctg gaataccgaa
    10861 atatcgtagc cctgcgtgtg cagcacctcg ttgagcccct cgatgaccga ggcggtgaac
    10921 cacaggcgga tatggccgct catcagtact gcgatgcgca gggagcggcc ggatttcagg
    10981 gccgcagccg agcgggagag tgagaaattg agtttgtcgg cgatggcgag cacgcgctcg
    11041 cgggtcttgg ccgacaccag atccggcctg gtgaaggaac gtgagacggt ggagatggag
    11101 acgccggcct cgcgggcgac atcctgaatg ctcgtagcca ttgcagcccc cttgcgttga
    11161 atggaaaatc gcaatacaca tgcacaactg actccagtgt acatgctgat tgattgatct
    11221 gcggcgatga caacgattgt attgcaaacg tttgcaaagt gtctatgtcg gcgttagatt
    11281 ggctttatat agccattatc ttaggtgaaa gaagattgct ggagccggag tgtgctcgaa
    11341 cttgaaacgc ctaaaatgac aacgtttgac atataatgat tcgacgttta ctatgaacat
    11401 aggcttgaac gagaacaggc cgtgcatgcg gttctcgatc ggcaagctcg cattgagttg
    11461 aactccgcag ccggcgcacg atgcagtgtc acaacgcagc ggactcaagg aggaggaaaa
    11521 gtcatgatca gcatggctgg caaggcgata cgcagatggt gggcgctgtt cgcgctcccc
    11581 acattcgccg cattcatcat cgggttcctg gtgcccttca tcatgggcgt ctacttaagc
    11641 ttctgcgaat tcaccaccgt taccgacggt gaatgggtgg ggcttaagaa ctatggcaaa
    11701 gcgttgaagg acaaggaatt cctgcacgcg ctcggcttct ccaccgcgtt caccatcgtg
    11761 accaccatcg tgatcaacgt gatcgccttc gccatcgcct acatgctgac caaggcgatc
    11821 aagggctcca cgctgttccg ctcggtattc ttcatgccga accttatcgg cggcatcatc
    11881 ctgggctaca tctggctgct gctcctgaac ggcgtgctag cccactgggg ccgcgccctg
    11941 acctataagg cgtcctacgg gttctggggc ttggtcatcc tggtgtgctg gcagcagatc
    12001 ggctacatga tgatcatcta catcgccggc ctgcaagccc tgcccaccga tgtgctggag
    12061 gccgcggccg tggacggcgc caacggacgg cagaccatgt tccgcatcat catcccgctg
    12121 atgatgccgt ccatcaccgt gtgctccttc ctgaccgtga ccaacggttt caagctctac
    12181 gatcagaacc tcgcattgac caacggtgcg ccgagcaaca tgtctgaagg tctggcgctg
    12241 aacatcaccc gcaccttcta cggccgtatg ggctgggaag gcgtcggcca ggcgaaggcc
    12301 gtgctgttct tcatcctggt cgcggtcatc gccctgattc agaacaagct caccacgagc
    12361 aaggaggtgg cagcctgatg aacgagaaca agcctgtgaa gcatggcgca ttatggaccg
    12421 tgctgtttgc cgttgtctcc ctgttctgga ttttcccgat cgtcctggtg ctgatcaact
    12481 ccttcaagca gaaggcgtac atctccaaga acgcgttctc cctgcctgcc ggcaaggcgt
    12541 tcgtgggact tgagaactat gtgcgcggca tcgagaccac gcagttcttc gcctccttcg
    12601 gctggacgtt gcttatcacc gtcggctcgg tggtgctgat tctggtgtgc acctcgatgt
    12661 gcgcatggtg gatcgtgcgc gtgaacaact gggccgcgaa gctgctctac accctgttcc
    12721 tgctcaatat gatcgtgccg ttccagatgg tgatgttcac cctctccaag atcgccgaca
    12781 tgctgaagct caataccccg tggggcctgt gcatcgtgta cctcggtttc ggtgccggtc
    12841 tggccgtgtt catcttcact ggcgtgatca agggcatccc gcaggagctt gaggagtccg
    12901 ccatgatcga cggcgcgtca gtgccgcgca tcttcttcca gatcgtggtg ccgattatga
    12961 agccgtcgat cgtctcggtg gccatcctcg aagccatgtg gatctggaac gactacctgc
    13021 tgccgtacct gacgctcgac ctcggcaagt acaagaccgt ctcggtcgcc gtgcaatacc
    13081 tcaagggcgg ctacggttcc gtggatatgg gcgccatgat ggcctgcctg gtgctggcca
    13141 tcatccccat catcgtcttc tacttggtat gccagaagta tatagtctct ggtgtcatgg
    13201 cgggcgcggt caagggttga gcccgacaat ttggctcccc tccctgaggg gagccaaaag
    13261 ctgtacaagg agtagttgat gaactggctg gcaccggact cgaaattcat gcgcgcgtgg
    13321 agcaatcttg ttgacggcgt atggatcaac attctgatgc tggtcacttc gattccgatc
    13381 atcaccatcg gtgcggccct gactgccggc catgatgctt gccgccgctc catcgagggc
    13441 gagggcaaag gcgtaacctc caactacttc aagtcattca aaaccaactt catcaaagcc
    13501 acactgctgt ggctgccgtt cttggtggcg ctgattgcac tggtctggtc atggatcgtc
    13561 ctgcagatca cgccgctgct catcatcaaa ttcgcgctga ccatcctctg gcttatcggt
    13621 ttcgaatggg tgttcgcgct tcaggcacgc ttcgagaaca ccgtggccgg caccttgaag
    13681 aacgccttca tcttcggcat ctcccacatc gcgatgaccg tggccctggt catcgtggat
    13741 gccgtgttcg tggcactgct ggcggccagc tggttccaca tgcccggtgg actgttcctg
    13801 ctggtgatcc ttggttacgg caccatgctg atgctccata tccccgtcgc cgagcgtgtc
    13861 ttcaagccat atctcattga ctaggcgggt tctcacgaat atgcggcgat cttcgaggtc
    13921 tgagcgctct ctgtccacgc aagcgcggta aagataacaa attaataaca gtttggggcg
    13981 gctggcggag cggggtgccg attgaggggt attagtgatt ggcttggaag ggggcttttg
    14041 gcatacgggg agtgttcggg ctgggcggtt gccccccgta agtgcttata tacataagga
    14101 atctggcaca ctgagtctca gtgccgtaat ggtatcattg caaataatgt ttgcgttcac
    14161 gtagaggcga acgagacact cgacaagtgg gtcgaggcaa aggacaagga gagatgcggt
    14221 gaagcattgg aagaagatgg cagcatcgtt gattgcaata tcaacgatgg tggcagtagt
    14281 tccgacgacg tatgccatgg aatcggaaga ttcccaacca cagacaaccg ataccgcgac
    14341 agtgcagact actaaggctg ctgaaccgac gctgctcgcc agctgggact tcacgggcaa
    14401 aaacggcacc acgaacagcg cgattgccga ttcgaccggc aagtacaacc tgacgctgga
    14461 ggacggcgcc aagatcgaac agtacggtga ccgcagcaac aacgaggcgc tctcactgcg
    14521 cggcgatggc cagtacgccc agatcgatga ccagctgttc aaggatgcgg gcgactcctt
    14581 cactctggag ttcgcgtcca agactcgtca cgacgacagc ggcaagttct tctcgttcat
    14641 cgtcggcaag gacggctcga acgacgccaa caccaccgat caggccaacg ccaacaagta
    14701 cctgatgttc tacaacagca agaccgccat caagggcgtt atctccaaca acaactgggg
    14761 taacgaacag ggatccaagg tcaccgtttc cggcaacgac aacagctggg ccgattacaa
    14821 gattgtcgtg gacggcacca accttgccgt gttccgcaac aatgccctga tcattttcaa
    14881 ggccaacacc ggcatcaaga tgagcgatct cggtgcgacc accgcctaca tcggcaagtc
    14941 gttctactcc gtcgatgagt actggaatgg tgcaatggat gatatcaagg tctacagggg
    15001 cgctgacctg accatgccga ccgccgttgc gatttccggt accggtgtgg tgaacaacaa
    15061 gctcaccctg attgagaagg actccaccaa gctcaccgcc accgtcactc cggacgacgc
    15121 cgtgagcaag aacgtcacct ggtcctcctc cgatgagtcc gtggccaagg tcgccgcaga
    15181 cggtactgta accggcgtca aggctggtac tgccaccatc accgccacca ctgagctggg
    15241 tggtgtgaag gccgaactgc ccgtcaccgt tgagccgatg aacgcccaga acgccgccgc
    15301 agccgacctc gatgccgcga ttgctgcgct gaaagttccg gcggccgaga atctgccgct
    15361 agtcgccaag ggcaccaaga acggctcggc gattacgtgg aagtcctcgg acgagaagct
    15421 cattacgtcc actaacgaga agtacgaaaa caggaccact ggtgccgatg acccgtatcg
    15481 tggtgctggc atcatcaatc gtccggccta cggcgacggt gattccaagc cggttacgct
    15541 gaccgccacc gcttcctaca acggcggtga gaaggtcacc aagaccatcg aggtcactgt
    15601 caaggagaag acccgcatcg cgcctgacac cggctatgcg gccgtcactt ttgagagcga
    15661 cagcaacggt ggagaaaagg cctgggtggc ttccactgag aagaacgatt tcttcacgtt
    15721 taagactcgc aacaatggcc aggcggtact taccaatgat gcagacacgg gtggcttgcg
    15781 tgacatgttc gtgctgcgtt cccacgaagg cgacaagtac tacctgattg ccactgatct
    15841 caaggtctcg tcaatgggct ggagccagaa ccaggttaac ggttctcgga aagttgaggt
    15901 ctacgagtcc accgatatga tgaactggac ccgtaccaac ggcgacggca acggcggcat
    15961 caccatcaac acgccgaacg ccggtatgac ctgggcgccg gaagcttact gggatgatga
    16021 cctgaacgct tacgtggtgt tcttctcttc ccgcatgttc actgatgaca cccgtaccac
    16081 tccggtcaag aacgacaaaa ccggcaatag ctcctatgct caggtgcgtt acgccatcac
    16141 ccgcgacttc gtgaacttca ccgagccgca gatgtggcag gacaccggct actcgcgcat
    16201 tgattccacc gtgcgtaaga tcggtggcta ctactaccga ttcaccaaga atgagcaggg
    16261 cggtgccgct ggcgattaca tcaccactgg taagagcatc ttccttgagc gttccaaggt
    16321 gctgactgca ccgaccaccg aggcatctcc gggtcaggac ccgaacaccg gttggcagct
    16381 gctcgagcag gcgttgctgc cgttcgaagg accagagacc atcaagctca acaaggatga
    16441 cgaactcaac acgaaggacg acgacggcta cattctgctg tccgacaact tcgcctaccg
    16501 tgcatttatg accacgggtg ccgagctttc caagaccacg tgggacaacc cgatgaccaa
    16561 gcgttacccg gacttcaaca acgaaaagaa gccggtcaaa gccgagccgg gcgctcaggg
    16621 ctacatcact cagggtgcta acggcggtct gccggacaag gtgcgtcacg gtgcgttcgt
    16681 gaacgtgcct gagtctgtgc tcaaggtgac gaagtcctgg accgctgcca acccgacgca
    16741 catcgaggct gttgactcca ccaccaaggc cgtgtacaac gccggcaccc gcgagctcac
    16801 agccacggtg accgccgccg ataagggcac gctcgccggt tcggtgaagt tctctgctgg
    16861 cgactggtcc aagaccgtga agctcgacgc cgaaggcaag gccactgtga ccctcccggc
    16921 cagcgtctct ggcactgttg cggttgctta cgacggctac accgatggtt tggtcaatcc
    16981 atccgatact acggttgacg gcattgaaca gggcaaggtc gatttggctg agctcaacaa
    17041 gcagatcgcc gccgccgaag cgctcaagga atccgactac acggccgatt cctgggccaa
    17101 gcttgccgcc gcgctgaaga ctgccaaggc cgcgctcgcc gctgagaatc agggcgaggt
    17161 cgataccgcc gcagccgacc ttaagaccgc aatcgaagcc ctgcagaagg ctccgaccaa
    17221 tccgggcgaa ggtgacggag ataagggcga cggcaataag ccgactaccc cgaccaccgg
    17281 cgacaagacc aacgtcaaca agcccggcag cgcgctgagc aataccggta cggccgtgct
    17341 cggcctgggt ggtgccgtgg tagcactcgc catcgccggc atctccctaa ccctctggcg
    17401 caagcgtcgc gcctgagctg ctacggcaac caagcccccc tctgacgagg ggggctgtca
    17461 gccgaaggct gactgggggg gagaccgcgg cgaagccacc gaaagaacgg catggccttc
    17521 ggccgattct gtatcccgcg gctgccgccg caggtctctc ccccgactgc ctaacggcgg
    17581 ccacctcgaa cgcaattaag gaccagcttt cagctggctt catatttgct cactgtcgtt
    17641 cgccttcgcc taaaaacgac tctggcgttt tcttcacggc tcagctcatc agagggcggc
    17701 cacaaaactt cgctccataa ctgaaggcgc aaccacaatc aaccctatca atgaggagaa
    17761 acatgggaaa gctgatacga aaggcaaccg gactcacggt cggcgtggca acactgctcg
    17821 ctggtctggt gctgccgatg acggccagtg ccgagagcgc atcgccaatc gatgccagtc
    17881 cgatcatcca ctattcattc gataacgcac tgacgtccaa gaccatcgct aacgagggca
    17941 gcgcggccaa cagcgatgcc accctatccg gcgacgccac ggtggccaat ggccagatca
    18001 acctgaccgg ctcgcaaacc attagcgtgc cgaccacggc catcgccggt aagaaggacg
    18061 ttaccgtctc catctggctc aagaacaatt acggcaacgg caataccgcc gccgcgtaca
    18121 tcggcgcggc caagaccggc aattatccgg ccaacggcta ctggctgctc aacccggcca
    18181 acccgagtgg ctacgcgaaa tccgtaatga ccaatgccac tgcggccgac ccgaataaca
    18241 gcccgtgggg caccgaagtc ggccctggat cgacgaacgc cgccaccacc ggcaccaagg
    18301 ccaccagcga tttggctctg tacaccaccg tcatcagcgg caccaacagc accatgagct
    18361 tctacctcaa cggcaagcag gttggagacg ccacctacac cattccggcc ggtggcctga
    18421 ccaattacgg cgatctcgtc gcctacattg gcaagtcctc ctacgctgac ccgaactcca
    18481 agctcgacgt ggacgattac gccgtatacg acactgccat cagcgccgca gacgtgacca
    18541 agctgtatga cgctcaggtg ctcgacaagg ccgaggccgc tgtcaaggcc gctgtgcccg
    18601 catccgctac cgaggacttc gcgctgccga ccagcgccgc tggcgtgagc atcgcgtgga
    18661 agtcggacaa cgcagccatc gccgttgaca acgctaccgg caaggccaca gtcactcgtc
    18721 cggccgcaac cgcagctgat gccgaggtga tcctcaccgc cacgtttggc aacaacgcca
    18781 aaaccgccga ctacacggtc ctcgtgccga agcagctctc cgatgccgag caagccaagg
    18841 ccgaccttga cgtcgtcacc atcgaggact ccgacgacat ccgtagcaac ttctccgtgc
    18901 ccaccaaggg caacaatggt tcgaccatct cgtgggaagt gaccggtggc aaggatatcg
    18961 ccacactagg cgaaggcgtg aacgacaaat ctcgaacggt cactgttaag cgccctgccg
    19021 ccggtagcga tgccgtcact gtgacgctca aagccattgc caagtacggt accgccaccg
    19081 aaacgaagac cttcaccgtc accattcagc cgatgcctgc cgccgaagag aaggacgagg
    19141 cctacgtgtg ggcgttcttc accggcgagg gcgtgggcgg cgagaaaatc agcctcgcgg
    19201 cctccaaggg caacgatgcg ctcgactgga acacgctgaa caacggcacg ccgatattca
    19261 cttccgagtt tggcgagaag ggtttgcgcg atccgttcat catgaagtcc aaggacggcg
    19321 acaagttcta catgctcgcc accgatctga agattgacgg tcgtgccccc ctcaacgggc
    19381 tgaatggctt tgctggtgca caggctaacg gttccaagta cattgagatc tggaagtccg
    19441 acgatctggt caactggtcc aagcaaagcc acgtcaaagt gagctctgat tacgcaggca
    19501 acacttgggc gcctgaggcc tactacgacg aggaaatcgg caagtacgtg gtctattggg
    19561 cctcgaacct gtacgacaac accgacgaga acagccgcaa gcagctgacc tacaaccgca
    19621 tggtgtacgt caccaccgat gacttcgtca acttctccga cccgacagtg tggattgacg
    19681 ttgatcgccg aggcggtgca ggcagtggat ccatcgatgt gaccgtgcaa aaggtagggg
    19741 atacctacta ccgcatctac aaagatgaaa acacgatgtc tttgcgtcag gagaagtcca
    19801 cagatttgac tgccgcaatt ggtggtgccg gcgtgaagaa ctacgccgat gcgcttaagg
    19861 gtagtgcatg gagcgaagtt gccacgaaca tcggtaaagg ccaggctaac ggttacggta
    19921 aaaccttcac ttccggcgaa ggtccatcgc tattcaaggc caacgatggc gatgtgaacg
    19981 gctaccagta ctacctgttc gccgaccagc cgagctatca tcaaggtcca aaccactatg
    20041 tgccgatggc gactgaggat atcgccagcg gtcagtggac cgttatcggc aataagatgc
    20101 ctgaggcgaa cttcccgacc aattccgatg gcggcaagcc gcgccacgga accgtgctgc
    20161 ccgtgacccg cgcccagtac cagaaggtgc tggaggcata cgccccggct gtggctgtga
    20221 agtccgttga cgcgctgtct gccgagacaa cggttggtgt ggctccgacg ctgccgg
//
LOCUS       DAWBMW010000015        20171 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_725_length_20171_cov_3.739660, whole genome shotgun sequence.
ACCESSION   DAWBMW010000015 DAWBMW010000000
VERSION     DAWBMW010000015.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 20171)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 20171)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..20171
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_725_length_20171_cov_3.739660"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    7674..7683
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    14133..14142
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4200 a         6036 c         5831 g         4084 t
ORIGIN      
        1 aattccctaa ggcgagtaga tgctatgtgg ctttgggcaa ggccagacgt gtgctatggt
       61 ggctgggatt actgctgctt tcaccagcac ttattttgtg ggcggaatgg ggcaccaatg
      121 gcgatgatga tactataatc tgcagagttc taacgttctc ttcctcttcg tgcatgaatt
      181 taactacggc aactgagtat gtacgtagac ttaccttagt attgattatg ggggtagtct
      241 gctggattgc ggcgcttgtc atctttggcg tgcgttcata tatgcgatct gtatatccgg
      301 aacttcgtga tcattcctct tccgcctcgc atgtccttgt gggaatcata tcgtttctcg
      361 tgctggttat ggtctttccc gttcttgtga taacactact ttccgctcta atgactctat
      421 gacgttttat cagaggcgca ttgcgtggaa tggctgaatc attccacgca atgcgatcac
      481 aatcatgatt gattaacggt tcggattttc aagagattca gcataggata atgccaatat
      541 accagcctcc gcttgactcg ggagtcggtc tgcatgtgac gcaatgagca gtgctttttt
      601 acttccggat acgttatcga atattccaag atcgattcca tcaacgagct ttcgaaatgc
      661 gatgaccagg ttgtccgcac ttcccttgaa ccgggactga tagaaccgtt taattcgatt
      721 cgtctcgctt tgcgcgaaca tgtcccgcat agcggtggac aaagaccttt cggaggaatc
      781 gctctgcatg gaccgcgcca gcaaccatgc atcacagtcc gcgagaacat cgctgtaact
      841 gaatcccata cgggcgtctt gctcgccgag atgagtgtgc agccatgacg ccaggtcctc
      901 tttcggggca ttcgccaggt agcttcccca aatctgcaac aaatccaggg cccatccgcc
      961 cagatcaccg agaccatagt ccattggatc gttcgtaacg ccccatgtca agtatcccaa
     1021 ggatgtggcg gccatatggg cgatgtcccg attgggcagc gtgcctttga tgacaggctg
     1081 tgcatcacat gcgacaccgc atgcctgacg cgacaaggcc aattcgctgg gagtaaccgg
     1141 ctccggggtg tacgcgtgcc atagtgcctg cgtgccggaa ctaccttccg accagtacgt
     1201 aggtttgcgg agccactgca ggatgagttg accgatgctg tcctcataaa ccgccacctg
     1261 ggtacccaga gcggcagggg ctttgtgaca ctcctgctcg ggtgtcgccg tcggtctgca
     1321 gaacacgggc tgatccgcgg ctgtccgacc gcttgatgaa cggggaggtt ccgtgccgat
     1381 tgacggcgcg ggcctccccg ttcgcgtcgc gcggccctag actggccggc gacggcgcgc
     1441 atagaacggg cgcgctggcc gagtgacaga ctcggatgat cgaaagggga catatgaatg
     1501 aggagaagga cacggacgga acgacggacg cgcacgaggg ggaccacacg cccgcggacg
     1561 gtgccgggac ggagggcgaa ggcggcaagc cggacactag gccgggcgac agactcgcga
     1621 ccatccgacg cacgctgtgg tggataggac tggcgctaca gctcccattc gctttgactc
     1681 tgatccattt tgatacccct tacctgatat tggagcgtat cctatgcgct attccgccaa
     1741 gactggcaag cgacccgtca tcaatcgtcg gcattcccgc acttgaaacg gaatggtgtc
     1801 gagaattcct ggcactagtc ctgggcgcag tttgctgggt tacagctgga gtaatgagat
     1861 tagttgcgga cagcccatca aaccatggaa aagaaaccct tcctgtacgc atcttcgccg
     1921 gaatcatcac catcatcgta ttgatggcca ccggtattac aggactcttc ctcggcatag
     1981 cggcctttta gaagtttccc cgtatctggc agaggaattc caaatcgaaa gcacaacagc
     2041 gaatgtgtgg gcagacgaca catctgccca cacataatgc attatgcgag ctaccgtctt
     2101 ggctgatcaa tgaatgcagc gaacgcccga gcactcactt cagcttcctg cttatcagga
     2161 agcttgtctg taccggcagc cttttttagg atgctttcgc tcattccaat atttgtcaaa
     2221 ccaagtgcat caagaccgtc cgccattgtt gaatatgcac tcaccacatt atctgcgctt
     2281 gatgcaaaac gttcgtcgta gaatcgaacg atacgatggg tctcactctg cttaagaaaa
     2341 attgaagcaa aacacgcaga gccagcaggc aggattcatc tacctcatcc tgtgcaccgt
     2401 ctccggcgtt gctgccggcc tgcagaacac aggctgatct acacaccagc gcatatgggg
     2461 aagccgctca catcgagcag agcttccccg tttcgtgtac atctttcacc atcttgcaat
     2521 aattcttaca tgtaaacaga gagttcttgc atgtaagaat tattgaaagg tgatgaaaga
     2581 acttactttc acgcgtacca atcattccaa cacgtatcgg gatgacggcc cattcccctc
     2641 gcggcgcaac ttgcccgcat cctctagctt cttcaacaac cggtatgcac tgatatagct
     2701 caggccaaac aaatccatga catccgccgt agtgacgaca ccatcatttt tttcggcgaa
     2761 ctccagaaca aggccactgc gccgggtggc atcgatgtca cgctggcgca cgtaagacgc
     2821 cagcccctct gaacgtttgt acactcgggc actcagcata tacgaacgag agctcccgct
     2881 accgaccccc tctaccaaac cagcttccac cagattctca acagcggaca cgatgcgtcg
     2941 atgctccaaa tgggagaaat cgcacaattg gaccacggta agcctacgat gctcacgcag
     3001 cagcgagagc acaatcagtg accagaccgg caatggagcc ccacgtcgac gttcctcgtc
     3061 gccaatcatg gtcacgaatg cctcgtcagg taccacgcgg cgcaagaaca agataacttc
     3121 ctcagcactg gactgtgaat aatccgggaa agaaccaccc gccgcaatcg acccggcata
     3181 gatggtatcc acgcctcgtc cggtgcgttc aacatatccg gcagctttca gaatcagggc
     3241 aagctgcgga ttacgggaac gcggttgtgc agtaagcagg ttatcttcgg agacaccctc
     3301 tatgaatcct ccggggttgg ctatggtcaa cccatcatca tcaaccagga acctcaccga
     3361 acccattcgt gcataatcgc gatggcagaa agcgttaatc attgcctcac ggaatgcccc
     3421 tcgatcgaag tctggcagat cgacacgaat caagccgctc atgacttcgt ggctgggatt
     3481 ccatggctcc atcagctcgc ctacacgatc aaacatatcc accaaaggca acacgaacgg
     3541 atccatattg acctttggag acatgccttt catcacttga aaaacagccg atgcggctgg
     3601 aaccgatcga tgaatagcat caaccgaacc aatggtgagc agaccagcaa cagatggctg
     3661 caatccatac gggccatcaa caaccagctc aagtgcccga tcgaaatcct catcagtaaa
     3721 agacaacaac gagttttgcg cattggcact gcgaatacgg cttctcaact tgttccgtga
     3781 gtctggattc aaatcgtcca accgagcctc tggtgcaggt tgcgcgctga aatcgtagaa
     3841 ccgttgctga gataaacggc taatgaattg ggaaacaaac agcggaacga cctcaggcga
     3901 gccgtctgct ttcagccggc gctgcaatgt cttcccctga ctggtagaca cgatttgctg
     3961 actgttatcc acctcaatac tgactatcgg cacctcgttc tcatacaaga gcgccacgcg
     4021 agcgggaagc tgcggaacag tcttattgaa aattagtacc gcaagtccat tgatattgcg
     4081 atgctcgtca catacgccgg tcacagtgcc atcgtcttca ataccgagat acagtgtgcc
     4141 gccttcggta ttggctagag ccactacggt gtcaacaatt tcatcatgcg attgaggacg
     4201 cttctgctcg ctcttgaact caaccgtcaa actttctttg gccggcaaca tgagcatccc
     4261 ctttcaccat cttgcaataa ttcttacatg ttaacagaga gttcttacat gtaagaatta
     4321 ttgcaagatg gtgaaagggg taaagagcaa tccatcctgt catctccagc aacttaaatc
     4381 cgatcatcgc tgctttcggc atatctgata tcagccgctg gtgccagtcc aatcacccaa
     4441 ttgaagctca acacggccaa agactctacg ccgcagtgga ttcgcgctcg atgagcttat
     4501 agcccacatt cacggtctgc gggaagaact cgtcgtccag gttctcgata cgccgcataa
     4561 gcaaggtcag cgcggtatcc gccatgccct tgaaatcggt ggccacggtg gtcaaagtcg
     4621 gcacgctgta catgctctgg ctcaggccgt cgaagccgat aacgcgcaca tcgcccggca
     4681 cacttacgcc atcgtccgcc agaccacgca gaataccgag cgcaataccg tcgttcaggc
     4741 agtacagacc gtcgtacttg cacttgcctt tcttggccaa gccgcgcccg gcctcgatac
     4801 cgccgtcaat cgaccacgag gatttgatga aatcacggcg ctcgtcaaac ggcagtccgg
     4861 ccgcgagaat cgtctccttg gcggcccgca tacgaatatc acgcgagccg ggaatgatgt
     4921 tgggattgtt gggatcgtga ttgtcgccga cgatgccgat gcgcgcacag ccacgcgcga
     4981 taaggtgttc gattgccgcc cgtgagccgg cgtcgctcgg cgcgttcacg gtgtcgatgg
     5041 gcaggctggt gtcgtagttt tccagcatga cggccgggtg ccgcttcagg atatcgaccg
     5101 cacgtggcgc attgatggcg atgatgccgt ccgaaaacgt gtacgacaga tcgttgtcgt
     5161 cggtaagata ccgggacagt tcgatgagca tgtgcgagtc gcgcttgtgc acttcctcag
     5221 ccacacacat ggccagatgg gagaaatagt cgccctcgag gcccgaggta aggaaggtga
     5281 tggtatcgga ccgcccagaa cgcagggacc ggccagacag gttgattttg tagccgagtt
     5341 tgcgggcggc gtcacgcacg cgctgcgtgg tttcgggctt gacgtaatcc ttgcctcgca
     5401 gggccgcgga ggcggtggtg gcggtcactc cggccagttc gccgacatcc tttaaggtca
     5461 ccatcgttac catctccgca tatcacgaac cttatcgata agaatccata gtaccgccca
     5521 catctcagcc gaggatgaga ccctcggtcg aggccctgcc cggcgactcg aacgccgcgg
     5581 tcaccgtggt gagcaccgac gctgcaaacg aaaacatgtc aaaccaagaa atggacatga
     5641 cggagtcatt gttttccgag agggatatgc cgcgtccacg ggcagacgtc acgtatcaat
     5701 cacgccgata ccgtcatata actgttttcg atgtgagaaa gctcatagct tccgaccaca
     5761 gaaatcagcc acagcaaccc cataacagcg ttatagttct gtttgtcggc gtcgacaaga
     5821 gccagcagcg ccgatgaaat gcacaccaag gatgtttagc gaattgttcg cagttcgcac
     5881 gattcgattt gattcgattc gtgcagcccg gatggttcac gggggcatgc cgtcgtgatg
     5941 tgcgtggata tgggcgatac gactctcgtt cgggccggca ggccatcgta caccagttac
     6001 gaaaggatcc gcccatatat ggcaaggttt accgagaatc gttggaccag cgcgattatt
     6061 cccgcactgc tgattcatat cccaatcggt accgtctatt gctggagcgt gttcaagcag
     6121 cttatcgctg accgtctcca cgccagcccc gcctccgtgg aatggggctt ctcactcgcc
     6181 atcttcttcc tcggcatgtc cgccgcgttt gccgggccaa tggtggagaa aaacatcaag
     6241 aaatccgcac tcgtctccat ggtctgcttc gtggtcggct tcgccggcac gggcgtgagc
     6301 atcgccctga acttcctgcc gggcgtgttc atctgttatg gcgcgatcat gggcatcggc
     6361 ctgggcgtcg gttatctgac tccggtcaag aacctgatgc tgtggttcgc cgacaacaag
     6421 ggcctcgcca ccggcatcgc cgtcgccggc ttcggcctgg ccaaggccat tgccagccca
     6481 ctgatggaat atctcatcgg cacggttggc ctggtcagca tgttcttcat cctcgccgcg
     6541 gtctacgcgg tgatgatgtt cgtcggcttc ctgctcatca agcgtccggc cggctgggtg
     6601 tatgatcccg cgacctcaca cgtcagccgc aagacaatcc tcaagaagcc cgtgttctgg
     6661 ggcatctgga tagccttcta tatcaacatc acctgcggcc tggccctcat cagccaagag
     6721 aaggacatcc tgcacgatgt gctcagggcc ttcccgcagt atgccaacct gagccccgcc
     6781 aagtttgccg ccgccatctc cggcatcatc ggactggtgc tcgccgtgga ctccgttttc
     6841 aacacggctg gccgcgtggg cttctccaca ctctccgacc acctgaagcg ccgcgagacc
     6901 gtctatcagg taatcttcat catgtcgatt gccgtgtgct tgctgcagat cttcaccaac
     6961 agcatcggca acgctctgct gtgggccgtg ctggccatgc tgttcctcgt caacgcgggc
     7021 tatggtggcg gtttctcgac gctgcccgtg ccgcttgatc agcacttcgg caccaaaacc
     7081 gtctccacca cgcacggtct gacgctgagc gcctgggcgt tcgccggtct gtccggcaac
     7141 cagttggcct cgttcgtagt tgcccacgcg cctgatcagg cccaccgcta cgccgcgctg
     7201 attccggtgc tcaccggcct gttcgtcgtg gccctgatca gcatcagctt ggtcaagtac
     7261 ctcggcgaca gaaacgtcac gaaggccgtc gaagaccgag gctgagctgc cgcaaaacaa
     7321 cgattctgcc ggagtcgggc gcatcgatcg catcaatcga tgtacgtcac gattttggca
     7381 gaatcgttgt tgttgctgtt gagtacctga ggtcgtgcgg cttacgcgcc gaccagttcg
     7441 agcatttggg ggatgctctt tttgccgaat tcgacttgct gggtgccgtc ggcgtgggtg
     7501 atgacgatgc agggcacgct catcgcgccg tactggtcct taagttcggg gaagtgcgag
     7561 acgtcgtagg cttcggcgcg cacggccggg ctcagggagg caatgcgctg ggaagcaagc
     7621 acggtttccg ggcacatcgt gcaggcgagc gagacgagga tcatgatgtt cagnnnnnnn
     7681 nnnggccggg accggccgcg ttgtacaggc cgagcacgaa cgagttgaac tcatggccgg
     7741 aaggtacgcc gtggaaggcc agaccggtga agaccagtga accattgccg tccttgccgg
     7801 ccttgccgtc gccctcagcc ggcacgcaga tgcgcacggt cgggcgggcg agcggcagga
     7861 cgccggcgac gtcgaatgtg gcgcggccgg aatcgtccaa cgaaccataa gtgggcagtt
     7921 cggtgccgtc cggcaaggtg acggacagcg gttcgccgac caccaaagaa gtaggcacag
     7981 aagcaccatc caccgccgtg atcagccctg cggcatcaac gacaaccgag ttcagcttgc
     8041 cgccggacag cgcaaccatc tcgccgatga aaccctgcaa ttccgccgaa agcggagtgc
     8101 cgtccagttc gagagcgagc gtgaccgggc gggtcatgcg tccgaagacc acgccaagct
     8161 gctgcttgac ggcggcggag aacagttcgc cgggcttctt ggcggcgctg gctgcggctg
     8221 cggcatcggc gctgcgtttg gcgggagccg gggcgggcgt cgtgccagcg gccgaggcgg
     8281 cggaggcggc cttggcctcg gactcctcat aagcggaagc agtgggacgc ggcggcacca
     8341 agccggtttt ctcgctcatc tgcttggcgt atcgctccag ttccacggcg gcaatcgcac
     8401 cgtcggaggt ggcggtgacc acttggcgca ggttcttgac gcgcagatcg ccggccgcgt
     8461 acacgcccgg caccgaggtt ctgaggtagt cgtgcgtgac cacgtaaccg tggtcgtcga
     8521 gctccaccac gccgcgcacc aaatcggtgg cgggcacata gccggcgaac acgaagacgc
     8581 cgaaggtgcc ggcgtcggcg ggcttccacg tttcggtctg gccggtggca aggttcagga
     8641 ttgaggcttc tcgcaggcct ccttggccgg cggtgacgcc cttcagctcg acctgataac
     8701 gcacgtcgat cttcgggttg ttcttggctt cggcggccac tgcggcatcg caggtgaagt
     8761 ccggttcgcg cacgagcacg gtgactttgg aggcgtattt ggtcaggaac acggattcct
     8821 cggctgcggc gaatccgccg ccgaccacca gtacttcctt gccggtgaag aactcgccgt
     8881 cgcaggtggc gcagtaggcc acgccgcggc cggcgtactc ggcctcgccc tcgaagccca
     8941 gcttgcgcgg gctggcgccg gtggcgatga ggatgccgaa ggtcttgagg tcgccgcgtg
     9001 aggtgtgcac ggttttgatg tcaccatcca cgtcgagacc ggtggcttcg gcggagagga
     9061 attcggcacc gaagtcctga gcctgctggc gcatggtctg ggtgagtgcg cggcccgtgg
     9121 tgcggcccac gcccggatag ttgacgactt cgttggtgat ggtgatctgg ccgccgaaat
     9181 cgtccttttc gagaatcagc acgcggtagc gagcgcgggc caggtacagg ccggccgtca
     9241 ggccagcagg cccgccacca atgacgacga cgtcatagag attgttggtc tgagtcatga
     9301 aaactccctt acatacactg gctcccctct ctgaggggag ctgtcgtagt gagactgagg
     9361 ggagcatatc ggatcaggct agctctcgcg agcactcccc ccagtcgctt tcagcgacag
     9421 ctcccctcag agaggggagc caaggttacc gcctattcag tgtcgatcac agctggccga
     9481 cgaggtcgag gctgggctcg atggtttcct cgccaggggt ccagttggcg gggcagacct
     9541 ggtcgccgtg ctcgtggacg aactgggagg cctgcacgcg gcgcagcagc tcctcggcgt
     9601 tgcggcccac gttggaggag atgacctcgt aggccacgac cttgccctcg gggctcacga
     9661 tgaagtcgcc acgctcggcc atgccatcgg cctcgttgta ggtgtcgagg tccttggcca
     9721 gcagggcggt cgggtcggca agcatcgggt actcgatctt ggcgatcttc tcgttggcgt
     9781 cgtgccaggc cttgtgcacg aagtgggtgt cgcaggagac ggagtagatc tcgcaaccga
     9841 tttccttgaa cttggcgtag ttctcggcca ggtcttcaag ttcggtgggg cacacgaagg
     9901 tgaagtcggc cgggtagaag aagaacaggg accagtggcc gagcacatcg tccttggtca
     9961 cttcgtggaa ctcattgttc tggaatgcgt tcaccttgaa atcggtcagt tcatgctgca
    10021 gaagagtcat catcggtcct ttcgggaatt gttaatcttc aagcgtttac caccacctat
    10081 cgtaatgtgg tgagccacgg ctcgcgcggg tgtaacgtat gagccttatc acaccacccg
    10141 caacccttgt gcccaaatgg ccgttgcccc aatcacgact atgcgcgaat actcgctaat
    10201 atggcaatca gcagtccgct gataatggag gtttcatggc tcagtcagac tttgaacagg
    10261 aatcgaccgc gaacgcaacg tggagccgca tgctgcaagg caaccggcgc tttgccgagg
    10321 gcaagcccga ccacccatgg caggacaagg agacccgcca gacgctgctt gatggccaga
    10381 acccggatgc cgcggtgctc tcctgctcgg actcgcgcgt acctccggaa atcatttttg
    10441 atcagggcct gggcgacctg ttcaccgtgc gcaccgccgg gcaactcatc gatagcgcgg
    10501 agctggacga cctgatgcac accatcactt ccgaagccga cggctcactg gaggccgcgg
    10561 atgcgatgga cgacctcgac gagcacatcg ccgccgccga atcgattatt ctgcgccagg
    10621 ccggcatgtc cgtctggcag gcgcgcgaag ccgagctcga cgcgcatgaa gacatcgagc
    10681 gcgtacacgt ggcccacatt attgaaactt tggtggagca gtctcctgtc atccagcagg
    10741 ccctcgccgc tgacaagctc atgatcgtcg gcgcccgcta tcagctcgat tccggcaaag
    10801 tggaagtgct gagcttctga gcgtgcgatc cgcaacatcg ccaacctgcg tgggatttgg
    10861 cgaatccttg gccatagccg cgagaaacag cctctctgca ctagtaatgc cgtccagcgc
    10921 tggaacgcat acggcatcgt cgaaggcgag caacgcatcg gaaacaccgg tcgacacgtc
    10981 gtccgcggta atgataagag tgcccttccc tttgccgagt ttcttactgc ccagccagca
    11041 caccccaatt tgacgtgcca ctcccctaga gttgaagcca tgtcacttgg tttgaacatc
    11101 ctcctgattt ttgtatttct cgttatcggt tccgtgttct ccggcaccga gttggcattg
    11161 gtctctctgc gcggctcgca gatcgagcaa atggagcagg aggacgctcg cggcgccaaa
    11221 gtcgccaaaa tcgcacgtga cccgaacacc ttcctgtcca ccgtgcagat cggcgtgacg
    11281 ctctccggct tcctgtccgc ctcgttcggc gcctcctcca tcgcccccta cctgattccg
    11341 gcggtcgaaa gctggggcgt gcaccccggc atcgccggcg gcctggtgaa catcatcctg
    11401 accctcatca tctcctactg ttccatcgtg atctccgaga tggtgcccaa gcgcatcgcc
    11461 atgcagcgca ccgagcagat cgcgcgcgcc gtggtgcccg ccatcgacgc cttcgccgcc
    11521 gtctgccgcc cgatcatctg gctcatcggc aagaacacga acggcatcgt ccgcctgctc
    11581 ggcttcgacc cgcagcagac cgagtccgag gtgtccgacg acgagctgcg cgtgctggtc
    11641 agctccaaca ccaacctctc caaggacgaa cgcacgattc tggacgatgt gttcgacgcg
    11701 tccgaaacca tcgtcgccga ggtgatgcgc ccacgtgccg acgtggtctt catcgagggc
    11761 gaccagccct tggccgaggc cgccgcgttc gtacgcgacc agccgtactc ccgttacccg
    11821 gtgaccggca aggatttcga cgacgtgatc ggcttcgtgc atgtgcgcga tctgctggac
    11881 gtgcgcgatc cgaacgccaa gatcgtgcgc gatgtggtgc gcgaaggcat ctccctgccc
    11941 ggcacctcca agctgctgcc gagcctggaa ctgctgcgta agcgcggcat ccatctggcc
    12001 gtggtcatcg acgagtacgg aggcacggac ggcatcgtga cgcttgagga tatgaccgag
    12061 gagcttgtgg gcgatattcg cgatgagtac gatctgccca gcgagaaggg cggcgaacgc
    12121 accacccgca ccgcgttcgt caatggcgtg gccacgatcg aggccagcat gaccattgag
    12181 gacttcgccg atctgaccgg catcgaattg gaggacggcc cgtacgagac ggtcgccggc
    12241 tacttcctgt ccaagaccgg caagatgggt gcggtgggcg acgtgctgca ttccgacgac
    12301 ggctacaaca tggtcatcac caaggtggat ggccgccgca tcgaaaccat cgaagtccgc
    12361 aaagccggtg ccccggacca tgcgccactg ccagccgaat cacaaagcga cgatgccaac
    12421 tagccacgtg atattcacca cctattcgtt cggcccgggg cataaccaaa aatcgtagaa
    12481 ttatatggac aggttccacc tactgaaaga aggaacatta tgacacttgc gtttgttgtg
    12541 ccgggccttg atgaagcggc cagtgagaag gccatcgcca tcctacagaa ccgactgagc
    12601 caggagcagg aggccgctct ggtcctgaag cacgcacact ggaacgtgac cggcccgaac
    12661 ttcatcgccg ttcacgagat gcttgacccc gaggtcgagg ccgtgctggc tcaggccgac
    12721 gagaccgccg agcgcatcgc cactctgggc ggcaccccgg acggccgcgc ggacgccatc
    12781 gtgcgcaacc gcacgtggaa gtcgttcgac gccgagggcc gcgtgggcac cgaggagtat
    12841 ctcaaagcgc tgatcgagta ctatgacgcc ttcatcgccg acgaccgcaa ggccatcgcc
    12901 gagctggacg agctggatgt gatcagctcc aacatcatcc aggaccacgt gcaggaactc
    12961 gaaaagttcc agtggttcat gcgctctcac ctggcataag taaaagcagt attccgtggc
    13021 tcccctcaaa gaggggagcc actttcgtct cacgtgaaat agagagtctc cacatacata
    13081 agttggaaaa gtgtataaaa ctccccattt tgaggttgga aaacgtagcc aaaaccgcat
    13141 tcttatggtt ggaaaagtgt aaaatatgcg ctataaacag ttggaaaacg tgcccagcat
    13201 agatgcaagg cctacaaatg actggagatt ctcatgctgc gaagaaaaat cgagacacag
    13261 ctggaagctt ggagatccgc gccgaaacgc actgcactgc tggtcaccgg agcccgtcag
    13321 attggcaaaa cctatatcat caggcacttt gcccaacagc attacgacca tatcgccgag
    13381 ctcaatttca tcgagaaccc tgacctcgtt gcattattcg atcggccccg cgatgccaaa
    13441 aacctactga tgaggctgga actggccgta aatacgccat tagtacccgg caaaaccctg
    13501 attttctttg acgaggtcca gcgttgcaag gaaatggtca ccgcaattaa gttccttgta
    13561 gatgatggtc ggttcgatta cgcgctttcc ggctcgctac ttggagtcga gctcgaagat
    13621 atacgctcgg taccggtcgg gtacctgacc gagctggata tgtatccact ggatttccaa
    13681 gagttctgct ggtcgcagca tatcggcaac gacgtattcg atatgcttgc cgaatgcttt
    13741 gccaagcacc aggaagtgga tgagttcatt catcaaaggt tcatggagct atactccaaa
    13801 tatctggtca tcggcggcat gcctgccgca gtcgactcgt tcgtggcaaa cggcagcgtt
    13861 gcggacgtgc gcactataca agagaacata aagcgtgaat atcgccgcga tatcagccaa
    13921 tacgcgcgca aagaagaccg gctgcatatc cgcgctatct acgatttggt gcccagcgag
    13981 ttaaataacc caaacaagcg ctttaccttc gccaaaatcg aaaagaacat gagattccaa
    14041 tcggttgctt ccgatttcga ttggctggtc aatgcggatg tagccgtcgc cgcgtacaac
    14101 gtggacgaac cgtgcacacc gttggagatg agnnnnnnnn nngagatgag caaggaacgt
    14161 aacctcttca aactctttta caacgacgtg ggtctgctta ccggctcgtt tctcaagaaa
    14221 accgcgctcg acgtactcga cggcaaccct gatatcaact acggttcaat ctatgaaaat
    14281 gcggtggccc aagagctacg cgctcacgga tttgacctgt actactacaa cagtaagaag
    14341 cttggcgagc tcgatttcct catccaagac cgcaacgatc acgtgctgcc gattgaggtg
    14401 aagtccggca aatcctacaa acgccatcgc gcaatggaca acgtgctcgc ccaccctgaa
    14461 taccatctca atacaggcta tgtgcttggc ccgtgcaacg tctcagtcga agggaatgta
    14521 acctacctgc ctgtatacat ggccggcatg ttccataacg aatgacgggt cgcacactta
    14581 gtcaacaagt acatgtcggt cagatggttc ggactggtca gagggcccgc tctgatcagt
    14641 taggtcggtc tgaacggact ggctgggctg gtcggcctga gtagcccgtt tggaccgatc
    14701 cggttcgccc ttgctggcct tcacactgct tcagctccag ctgaagttcg ctgatgcgcc
    14761 gttcgagttc gtcgatgcgc cgcacgtgtg gcgaagtgag actaagggga gccgagagat
    14821 ggctcacagg ccgagggcac cggccacggc catggcctcg tgttcgggat cggcctggcc
    14881 gatgtgtacg ttgacgtgaa tttcgtcatc gcccggctct tcgagaatgt cggactggga
    14941 ttgcagcatg ctcggcttca tgaagtggcc ttggcggtgg gaaaggcgtt ccatcacctc
    15001 gtcgaatgtg ccctgcatgt agacgaacac cacgccttga cggcgcagct tgtcgcggta
    15061 gatcttcttc aacgccgagc aggtgaccac ggcgggctcg ccggcggcca cgcgctcgtc
    15121 gatccacgcg gcgatggtgt cgagccaagg ccagcggtct tcgtcggtga gcgggtgtcc
    15181 ggcggccatc gagatggtgg cgcattcgga ccatgcgaac gaatccgatc aggccatcag
    15241 ccacatcgcc ggcactgaag cctgacccgt catcgtcaca gcaccacgcg gcggttgagg
    15301 tcgcggcgca gcgtcttcca gccgggtagg taggcggtca tgcggcgctg gaatccggca
    15361 ccgtggccgc tttcccacaa atgcgtcatc tcatggacca agacgtattc caggaattgc
    15421 ggttccatca gtcccaattg aaggttcaga cggatgcggc cggttcgcgg tgtacacgat
    15481 ccccagcgcg aggtcatgac ccgtagcgtg atatgcgtgg gctgccggcc gacgaccggc
    15541 ccccacttgg ccagcagctg tggcaacgcg gcgttgatcg cggccgccgc ttgtcgtttg
    15601 cgttcgtcgg tccagatttg ggagcggtca aaatacggtt ggacattcgt accggcttgg
    15661 cctggcccgg cgtcaccaat tcccggacga ctgatatcac cggttttcgg acgaccggca
    15721 acgcctccat catggccgct ggcaccagct acaccacgac ctccgttctc gaagccggcg
    15781 ccctcgttca atgccatgat ggagcgccgt tgcgcctcag ccatgcgctg ctgctgggcg
    15841 agaatccacg aatgtcgctc tttgacgaaa tccgcaatgc gactctcgct catccgcatc
    15901 ggtgcggaga tttcgacgcg gccgctcggc ggtttgatgc gcagatacag gtttttgatg
    15961 ggtttgcgcg tcaggtgcag atggatgcca tcaatatcca acgactgccc ggcggcagcg
    16021 gaccgaacgg atttcggcgc accggacttc ggagcggtgg attgacgacg gcgataaggc
    16081 acgcaagtca ttctcgcaca acggcggaca acgatggcgg cccgcccgga acctaacgtt
    16141 ccgaacgggc cgccatcagc aatgaacggt gctgtcggca acggctacaa accgtcaccg
    16201 agccattgag tcacccaatc agcaaccggc cgcggtcagc acctggtcga ggatctcgac
    16261 gccctgggtc gcctcgtcgg cggagatgac gcaaggagga gtgacgtgga tgcggttgtt
    16321 gtgcacgccg agaatcaggc cgcgcttgtc gccttcggcc acgatctgct tcatcgtggc
    16381 gtcgtccagc ggctccttgg tggtgcggtc ggcaaccatt tccacggccc agaacacgcc
    16441 cttgccgcga acctcgccga tcttcgggtg cttgtcgtac agagcggtga gctgcgggcc
    16501 gagcacgtcc ttgccgatgg tgtaggcgtt gtcgacgatg tgctcctctt ccatggcttc
    16561 ctgggtggcc acgatggcgc ccatggccag cgggtgaccg gagtaggtca ggccgccggg
    16621 gaagacgatg tcgtcgaagc ggtgggccac cgggtcgctc ataatcacgc cgccggcggg
    16681 cacgtaaccg gagttgcagc ccttggcgaa ggagatgatg tccggcgtgt agtcgtagtc
    16741 gtcgagtgcg aaccagaagc cggtgcggcc gaagccggcc atgacctcgt cggtgatcat
    16801 caggatgctg tacttcttgg tgatctcgcg cacgcccttg aagtagtcct tgggcggaat
    16861 gatgatgcct gcggtgcccg gcacggattc gagcagcacg gcggcgatgg tgtccgggcc
    16921 ctcgaactgg atcacgtgct cgaggtgagc cagcgcacgc tcgcactcct cttcctgggt
    16981 ggttgcggag aactcggagc gatacaggta cggggtgaag aagtgcacgt ggccgcgaga
    17041 gtagtcgttg ggcttacggc gccagtcacc ggtggcgctg atggccgcac cggtgttgcc
    17101 gtggtagcag cggtaggaag acagaatctt gtcgcggcca gtgaacaggc gggcggcgcg
    17161 catcgcgttt tcattggcgt cggcgccagc gttggtgaag aacaccttgt cgaagccttc
    17221 cggagcgcgg cgggccacca gggaggcggc gcggccacgg gctaggttcg cgtagtcagg
    17281 tccgacggtg cacaggagtt ccgcttgctt cttgatgccc tcgatcacct tcgggtgctg
    17341 gtagccgatg ttgacgttga cctgctggct ggtgaagtcg agcatttcac ggccgtcgcc
    17401 gtacttcagc cacacgccgt gaccggttac cacttcagtg ggatccaggt tgcgctgtgc
    17461 gtgccacgaa tggaatacgt gtgcgcggtc cagcttgtaa gcgtcgtccc agatttccgc
    17521 atcctgcgaa agatcatgcc ccatgatgtg tcctttcctc gatattttct cgaagtgccc
    17581 gatgccgtct cccgtggatc gggtcagggc agcaacgcgc tgtgaatgtc attatgagag
    17641 ttgaacattt cgcgtcaaac tcgaacattt aataatgtca cagtagtggc gaatacgaaa
    17701 tcattttgaa acataggaac aagaacaata attgtatcca gattatgtaa tgtttatgtt
    17761 attcaaaaac atactcattg cacaatgcgg cgctaacatt tgactaccgt tgccacagtg
    17821 accgttacta tcggcagtca agcagccagc aacaagccgg caaccgccat cgatcatcag
    17881 ccggcggcaa tcggcaacat tcacgcagac gcgcgctcat cacgctcgcc gccaagatcc
    17941 gggaggcaga tcatggatac gccatcgcca tcaaccgcat caacaaccgc acatgccgtc
    18001 ggctacaacg atgagccaag cattacggcc gctcccctgc gttccaccga cgacctggcc
    18061 gttgatgccg gccagttatc ctcactcatc ctcaccaccg aggcgacggc caccccgcaa
    18121 cgcatcgtcg aatcggtcac ctcgctgacc tcctccggca tacttgcgga aggccagcgc
    18181 atgcccaccg tacgcgatct cgcccgactg ctacgcacca gtccggccac cgtctccagt
    18241 gcctaccatg ccctcgccag gtccggcata ctgcgctccc gcgggcgcgc cggaacattc
    18301 gtactgcggc agtcgcgcac gcatcgctac gatgccggcg acttgctggc cgaagccccg
    18361 gccaccgcgc cggaccgacc gattatcgac ctgtccaagg gcacgccgga cctttcgctg
    18421 ctgccggaca tccgcccgtt tctcggcaag ctcggcacgc acaaggcatt cgtcaattca
    18481 tacgacgggc cgaccatcct gcccatgctg gaacagattt tgcgacgcaa ctggccgtat
    18541 gagccggagg cgatgactat ggcatccggc gcgggcgacg gtttggcgca caccatgaac
    18601 gcttttgttc agccgggtga ttttgtgatt accgaggcac cggaatatcc gctgattctc
    18661 gacatgatcg aggcacatgg cgcgatggcg ttcggcgtgc cgatggacgg gttcggtatg
    18721 ctgcccgacg cactcgaccg tgcgctgacc atgctggaat cgcagcgtct ggcggtgccg
    18781 cacggcggcc atcaggtcag tatgatcctg ctgcagccgc gcgcccagaa ccctaccggg
    18841 gccagcttca gcccgcaacg catcgacgct ttggcggacg tgctgctcgc gcactacccg
    18901 aatggcgttg gtatgccgct gattgtggaa gacgatcaca gtggcgatgt ggccaatgcg
    18961 tatgcgacat cgttggcgca acgattgccg caacacgtgg tgcatatccg cagtttttcg
    19021 aagtcgcacg ggccggatct gcgcttggct gcgctgtctg ggccggagga tgccgtggag
    19081 gcgattatcg cccaacgacg cttgggatcg ggatgggtga gccggttcct gcaagagatt
    19141 ctgtatgaaa tgctggccga caaagaggcg ttccacacgg tgaccaacgc ccggcatatc
    19201 tacgccaccc ggcaaaagac gatgcacgcg ctgctgctgc gtcaaagttt ggacgtgcat
    19261 accggtgacg gactgaatct gtggattccg gtgcgcgacg agccggcggc gctgcgactg
    19321 ctggccgaac agggcatccg tgtggccgcc ggcaagccgt tcctgccgag cctgcgtatc
    19381 agcgccgacg ccaccggagc cgacgcgggg gccggtgcgg gcgtggacgc gcatgccagt
    19441 cgaggcatcc gcgtcaccat cgcacagcag ggagccgtca acgaacaagt ggccgccgcc
    19501 ctggctcagg cggcggccgt aacgccgaag acttcgacga atcactcgta attcttcatg
    19561 aactgcgttc gtacgcgcgg cgaggagccg ccccgtctgt acggggtgat caacgggctg
    19621 ctgcgcatgc gcaacgggct gcgcaggtcg caggagctcg acgacctggt cgacgtgctg
    19681 gcctcctcca tcggcgccct gaccaatccg cgcaccaact gcccacaccg gagaaggtcg
    19741 cgcaggagaa gcctcgctgc tcgagatcga cgacagcttc cagaagatcg tcctcgtacg
    19801 cgacgtggtc aaacccgtcc gcgacgagca cggcatcctc acgatgagcg tctacgattt
    19861 cctcctcgac ccggacagcc tgacgatgtg acgccgtgga ttggctacaa cgtattagac
    19921 agtccgatga gggacgaatg acatgagatt ggaggccgac gatggccgac ccgaaacatc
    19981 cccgccacta cgacgaggcg ttcaaacggc agatcgtgca gttgtacgag agcggcaagc
    20041 catcccggaa gatccgccgt cgtggcccga catcctcagg accatcgagc tgggctactc
    20101 caacgccaga ctcgaggcgt ccgacaacag gatcaaggtc accatccgca tggcctacgg
    20161 cttccaccac g
//
LOCUS       DAWBMW010000016        19441 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_754_length_19441_cov_3.435520, whole genome shotgun sequence.
ACCESSION   DAWBMW010000016 DAWBMW010000000
VERSION     DAWBMW010000016.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 19441)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 19441)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..19441
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_754_length_19441_cov_3.435520"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6029..6038
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9505..9514
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3809 a         5506 c         6066 g         4040 t
ORIGIN      
        1 tgaccggcat cgctccggct ccgcgtggcg ttccgcagat cgaggtcacc ttcgacatcg
       61 acgccaacgg catcgtgcac gtgtccgcta aggacaaggg caccggcaag gagcagtcca
      121 tgaccatcac cggtggttcc ggcctgccga aggacgagat cgaccgcatg gtcaaggaag
      181 ccgaggctca tgaggctgag gacaagaagc gcaaggaaga cgccgagacc cgcaaccagg
      241 ccgaggcctt cgcctactcc accgagaagc tcgtcaacga caacaaggac aagctctccg
      301 acgacatcgt caaggaagtc accgacaagg tcaacgctct gaaggaagcc ctgaagggtg
      361 acgacaccga gaaggtcaag accgctcaga ccgagctgat gaccgccgcc cagaagatcg
      421 gccaggtcct ctacgcccag cagggtgccg agggtgctgc cgccggtgcg gctggcgctg
      481 acggtgccgg cgcttccgct ggctccgcct ccggttccga cgacgacacc gtcgaggccg
      541 aggtcgtgga cgacgacgac aaggacaaca agtgatgtcc gagttcaaca aggacgacta
      601 cctgaacgac ctgccggatc cctcagatgc tgaggctgcc gctcaggcct cttcaggggc
      661 tgacgcctcc gccgagtccg gctctgcgca ggattcggct gcccaggctc cttccaatga
      721 aggagccgac gcggctcctg ccgcagctga gggagagaag actggcgaag gccagtccga
      781 ttccgcagac accctcaccc cgctcggcaa ggccaagaag gaagccgcag actacctcga
      841 agccctgcaa cgcgaacgtg cggagttcat caactaccgc aatcgcacgc agaaggagca
      901 ggagcgcttc cgccagcacg gcatcatcga cgtgctgacc gcgctgctgc ccgccctcga
      961 cgatatcgac cgcattcgcg aacacagcga gatggacgac tcgttcaagg ccgtcgccac
     1021 caagattgac aaggccttcg agaagttcgg tgtcgagaag ttcggcgaaa agggcgagga
     1081 cttcgacccg accaagcatg acgcgatcct gcacaagccg gacgcggatg ccgaaaagga
     1141 aacggttgac accgtggtcg aggccggcta ccgcatcggc gatcgcgtaa tccgcgccgc
     1201 ccgagtagta gtagcctccc cgcaaaactg acatccagct cgctgcaatg agttgatatt
     1261 taccttggct cccctctctg aggggagcca atcataaaga cttttctaga aaggagacat
     1321 gaatggctga aaacgaatgg ctcagcaaag acttctacaa agtccttggt gtctccaagg
     1381 acgcttcgga cgacgacatc aagaaggcgt accgcaagct cgcccgcaaa taccatcctg
     1441 acgtcaacaa gaccaaggaa gccgaggaga agttcaagga catctccgaa gcctatgacg
     1501 tgctgagcaa gaaggaagat cgccagaagt acgacgcgat tcgtcagttc ggcatgggcg
     1561 gtgcccgctt cgccggcggt tccggcggct tcgatgcttc cggtttctcc gacatctttg
     1621 gttccatgtt cggccagggt gccggcggca atggctcgcg catccgcttc tccacttcgg
     1681 gcggcggccc caacaacatc aacgacatct tctcgatgtt cggtggtgct gcaggccaag
     1741 gcggccagcc gtatggcggc tacggccaat cgccgtacgg caatgccggc ggctatggct
     1801 atgaggaggc cccgcgcccc gaaaacggcg aggaccgcaa ctccaagatc agcctgaccc
     1861 tgcgccaggc ggtcaagggc gctaccgtgt ccctgtccgt ggacggcaag aagttcaagg
     1921 cccacgtgcc tgcgggcgtc aaggacggac agaaaatcaa gctggccggc aagggcaagc
     1981 caggcatcaa cggcggcaag gccggtgacc tgtacctgca cgtcaccgtc aagcccgatg
     2041 ccacattctc gatgcgcggc catgatatcg tcatggacct gcccgtcacc gtgggccagg
     2101 ccgtggccgg tggcaaggtc gaggccaagg atatcgacgg caatcccgtc accttcaagg
     2161 tgccggcagg ctccagctcc ggcgcggaag tcaagctcgc gggacttggc gtccagcgca
     2221 gcaaccagcc gggcgatctg atcggccgtg tgcagatcat ggtccccgcc aagccgggtc
     2281 tggccgtcaa gcacgccgcc aaagaattcg acgaaaaagc cggcgactat ctcagcctgt
     2341 aatcaggagg tgaacaatgg cgcggttagc caaccagatg aagcagttgt acgcgatgtg
     2401 cgcaaccgcg ctcgtgatgg gcagggccga tctcgacgga gccgatgacg tcggcttcga
     2461 catcgaactg cccatcttca ccgtcggcca gaccgccgag ttggccaaca ttcaccccca
     2521 gaccctccgt cagtacgacc gcctcggttt ggttcgtccg cagcgcaccg atggcggcgc
     2581 acgccgctac tgcctgcgcg acattgcccg cctgacccga gctcagcgcc tgagccagga
     2641 tgagggcatc aatctttccg gtatcgcgcg cattttggtc ctcgaagaag agaatcgcca
     2701 actgcgccgc gaagtcaaac gcatgcaaac cggcgaccag gagagcgttt tcgccgcagg
     2761 ccgtgacggc aatatcgtgg aggtacaacg ctccaaccgc gcccgcctct ggcgcaacgc
     2821 catccgccgc gaaacccgcg aactgcccgg ccgcgatact gccgccgata tcgctgccgg
     2881 cgccaccggc gactccaact acactcccgc cgactccaag tccatggtcc tgtggggttg
     2941 gtgagtctgg ccgcattgct tcgattcctt ctccattcgt aaaactgttt gttatctcac
     3001 atcgggcagc tgccgaaaaa aaagcacgaa tcttcttgac ggagatgtca aggtttgtta
     3061 cggtgacgtg cgaaaaccga ttcgtaaggg gtgctttagg ctgagacggt atgtgccgaa
     3121 cccttcgaac ctgtgagcta acactcgcgt agggaatgcg acggttgaac tcctctgttc
     3181 ggctcggcat cccatatgcg cgttgagaga gaggaagcgt atgtcaatac gcaaggagct
     3241 gaccgcccag aacgtactgc tgggtttcca gcatgtgctt gtttccaacg tctggcttga
     3301 tccggtgttt gtggccggag caatcggctt acctattgcc ctgtcgtcga acatgattaa
     3361 cgccatcttc atcgtctctg gtctggtcac tttggttcag gccactcggc tggttcgcct
     3421 gccggtggtg caggggccgt cagctgcgtt tgacgcgttg atgatcgcgg caggtacggc
     3481 cggcatgctg ggggcggcga gttcgtccat tctcatcgct tcgcttgtgt tcctgttgct
     3541 gtgcctgacc ggtgtgatag agcggatgcg gttcctgttc tctccgatga tttcgggcgt
     3601 ggtcatcttt atggtcggtg tctcgctgtc tggattcacg ctgagcgagt ttctaggcgg
     3661 ggctcccggc gacaagacgt tcgccgatcc gcacatcctg accgtgtcga ttctgaccac
     3721 ggcgattgtg cttgtcctgt cgttgctcgg caagggcctg ctcaagtcgt tctcattcct
     3781 catcgcgctc gtcgtcggca ccgtggtcgc cgcagcgttt ggcatggtgg acttctctcc
     3841 tgtggcatcc aagggatggt tgggcctgcc gaccttcctg ccctacggtc cgttcacatt
     3901 tgactggaag atcttcatcc cgttcttcat cgcctacatg gttgccgtca tggaagctct
     3961 cggcgtctat caggcggctt ctgagattca gggcacgccc ctcgaacgca agcaggtgcg
     4021 ctacggtctt gccggcgagg cggccggttc agccatttcc tcgctgattg gtggattcac
     4081 caccaccgcc tacccgcaaa atgtcggcct gctcaacctg accggcgaag gcaagttgcg
     4141 cacgcgcacg cctgtgatca tcgccgctgt gctgttgctg attctgggct tcgtgccaaa
     4201 ggcgggcgcg ttgctgtccg tcattccctc ggcagtcgtc ggaggcattt tcctgccggc
     4261 tgcggccagc ctaatcgtga ccggcctgcg cgccctgcgc aaggtcgctg atgacgagcg
     4321 ccgtcaaacg gtcatcggca tgtctctgtt gctcggcatc gccctgccca aggcgctctc
     4381 cggcctgacc ggcggcgcaa gcgtgttctt tggcaacaac atcatggtcg gtgtattcag
     4441 cgccatcatt ctcaatgcgc ttttcgtcgc tcttccggat ttggtggcga agcatcgtgc
     4501 gccggcgtcc gtggatgttc cagcagccga tccggcggat gggactggcg ctgcggcgta
     4561 cgatgaacga taatgctggt tgatattgga tattgacagg agcgatgcaa tgactgatgt
     4621 gatggatgac gcgcgtcgta tcaagcgcgg gattctggat tttatccata cctatggctg
     4681 tgagcaggcc ccggaagagg cgtttggtgc gttggcgcgc gacgtgttca tctatcagta
     4741 cacgtatgat ctgccgtacc gtcgttactg cgcggctaaa cgcgtcaccc ccgcaacgct
     4801 caaggattgg cgggagattc cgccgatgcc ggtggacggg ttcaagcacc ttgacctttc
     4861 gaccataccg accgagcagt gcaaagccgt attcatgacg agcggaacca cgcatggcgg
     4921 caagatgcgt gggcgcaact tccaccccga tcttgaggtg tgggatgaat caatgattgt
     4981 gccgttccgt catttcatca tgccggatcg agagcgtatc cgcatcgccg tgatttcgcc
     5041 ggcatgggac atgaaccaga actcctccct gtcgcgctac cttaccaagg ccgtggagtg
     5101 ctgcggcagc gaaggcagcg gtgtgttctt cgacgagcat ggactcaagt tcgatgaaat
     5161 catcgccttc cttaaccgtg cggtgtctga cggtgaaccg gtgatgctga tgggtgtctc
     5221 gtcgtcctat ctgtatctgc tcgattacct gcatgagcat gatctgacgt tcgcgctggc
     5281 ccccgatagc cggttgttcg acactggcgg cttcaagagc actaagcgcg acatcaccga
     5341 agaccagatg cttgatgagc tggaacagac actgggggtg ccgcgccacc actgcgtcaa
     5401 catgtatggc atgaccgagt tgagctcgca gatctatgac caaaatattc tgtcgtggta
     5461 tacggatggc accagcaact atctcaaggc gaaccccgcc tgggtgcgca cggtcttcct
     5521 cgatccggaa acgctgactc ccgtcaagga tggtgagacc ggtgtgatag cccattacga
     5581 ccttgccaat tggaattcat gcatcggtat tctgaccgag gacctcggcc atcggacccc
     5641 ggacggattc ctgctgcagg gcagggcgaa gggagccgag gctcgtgggt gctcaatcgc
     5701 ggtagacgag gtcatcagtg ccaaccgttg atgaatccgt gagtcaggcc attgatgtct
     5761 tccacctgcc ctcgggagtg gatgtttcgg attacgaaat ctatgaggtc gccacatcgg
     5821 atggggtgaa gcgtttgcgc tatccgcgac tggatgggcc aaaggtgact tcccttgcca
     5881 agcagcttgt cgatgttcgc aaccacacgc ttgccgcgat gagtgtcaac gatattctcg
     5941 acatcgtggg caagccgtaa cgttgtatcg gcctccatgt tgacgacgca ccgccccggg
     6001 atgttgtaca ggatgaccgg cagatcgann nnnnnnnntc gacatcgtgg ccgacgcggc
     6061 ccaactatgg gccgatccgg attttgagct gcggcgtcag gctgaactgc tgattcccgc
     6121 aatcaccgga tatgagcctg acatggtgcg gattgagctc aaacggtata tgcgccagtt
     6181 caggcgtcgt gaactgctcc ggttccttga ctccgaaatc ggtcagccgt ctatgctcga
     6241 cgaattccgg ccgaacaagg cggggggata ctctaaatat gtgggcccag cgctcaccta
     6301 tcaggtgttc tccagcaatg tgccggggat tcccgtgtgg agcatggcga tgaccctgct
     6361 ggtcaaaggc gcgattcttg gcaaatcttc attttccgag ccggtgatgc ctgcgttctt
     6421 tgctcgcagc attgcgatgg tcaattccga cctcgctgat gccattgcgg tggttccatg
     6481 gaagggcggc tcgcagcagc tcgaggattc agccatcgat gcggccgatg ccgtgattgt
     6541 ttacggttct tcgcagacta ccaaattgat tgcgggcaaa gtcgccgggt ccaagccctg
     6601 tctaggttac ggagcaaaag tcgggttggc gttcattggc cgagaggctt tgcgtcctga
     6661 tacctatgcc gataccgtgc atcgtgtggc tgttgacatc gccacatatg accagcaaag
     6721 ctgcctggct ccgcagaccg tgttcgtgga gactgatgga gccctcaccc ctcgtgaagt
     6781 ggctcagctg ctcggtggcg agcttgagaa tcagcagcgc aaatatccgc gctccgtatt
     6841 gagtgacgcc gagaacgtgg ccattcagcg ggcgcgcacc gacgctgaga tgagagcctt
     6901 gatgggcggg aaggccgcgg tgttcgccag tggccatagc acggcatggt cggtgctgta
     6961 tcgagaactt gacggtagcg gtgccgacga ggatgttgct tcgctgatgt cgccgttgaa
     7021 ccgcaccgtc aacgtggtgg cggtgccaga tttgttggac gctgccagaa gactgacttc
     7081 ctgccgtggc tggctgcaga gctgcggtgt cgctgtggat tcaaccagac tgtttggtct
     7141 tgcagataca ttggccgagg tcggtgtgaa tcgcatctgt ccgctcggtg agatggatcg
     7201 ggcgaaatcc ggctggcatc atgatggggg cttcaacctc atcgatctgt tgcgcgcggt
     7261 ggatgtcgaa cgcggcagcg acgcgtacag cgattcgttc gatatggata tggaataggc
     7321 ctgtgctgtt gcgggcagca gaggctggta gcagggcgaa aggcaagggt aatgacagag
     7381 caaagcgata tggcacggcg tccaatggtg aatctggatg gcagaacagc gatcatcacc
     7441 ggttccggca agggcatagg agcggctgtg gcgaaatgcc tggcatcgca cggagccgct
     7501 gtcgtcatca attacgtaca tgacgccgcc agcgcgcagc gcaccgttga agacatccac
     7561 tcgctgggag gcttggctat agctgtacag gcggatgttt gcgatgaatc gcaatcttcc
     7621 aagctagtgg gtaccgctgt cgactcattc ggcggcgtgg acatcttagt gaataatgct
     7681 ttcggacgct tctcctttga cccgcgtcgc cgttccactt ttgctggagg cgattgggat
     7741 gagtttagcg cacagatcga gggatgcctg catggtgcat atctgatgtg ctcacatgtg
     7801 gtgccgctga tgcgagccca gacctccggg cgtatcatca acatctccag caatctggtc
     7861 gattcgccca tcgtgcccta ccattcgtat atcactgcca aaggcggctt ggtcggcatg
     7921 acccgctctc tggcccagga gctgggcggc ttcggcatta ccgttaatgc cattgccgca
     7981 ggtttgacgg ccggaaccgc atccagcgca gccaccaccg aggatgttcg tgaacgaatc
     8041 attgccatga cgcctcttgg ccgccttgcc cgtcccgatg acatcgccgg aggggtcgcg
     8101 ttgttggcgt ccgacatggc cggcttcatt accggacaat gtctgcacgt cgatggtggg
     8161 ctgaccatgc gctgaggcgc aggggcggtg tgctgacgcg ctatggttgt cgcatccgat
     8221 tggtgcaatg gaatgcagca agagtaaagg cgtcaattgg cgtgcgggca tgtggacgga
     8281 ggtaactatg ctgctcaaag ccgtgttctg ggatttggac ggtactctca tcgattctga
     8341 gccgctctgg catgacggtg agattgagat cgcgcacaac aatggcggcg agtggaacga
     8401 ggacctcggt tgggagtgct ccggcactcc ggtgccacac gtggcagagg tcatgattgc
     8461 gcatggctgc acattgagcg ttcccgaaat cgacaagcag ctcaaggatt atgtgttcaa
     8521 ggcggaggtc gaacgtctgc cgtggattcc tggtgtgcaa gacgtgctgc attcgctcaa
     8581 ggaagccgac atcccatcca tgctggtcac tacttcgcct cgccgcatgg ccgagaacat
     8641 catgaagcag gctgatggat tgcttgccag ctacgtctgc ggcgatgatc cgtacgagca
     8701 caagccgagc cctgccccat atctggccgc ggctgagaga ctcggcatcg cgcctgaaga
     8761 catggtcaag tgcgtggtaa tggaagattc ctcatccggt ctgcgctccg gcgccgcctc
     8821 gggggccaca ctgattgcgc agactggctg gattcgcacc gacacttccg gccttggcca
     8881 gtttgcctcc atcgactcgt atgagggcat cgatgcggcc gcgctggacg cctttgtgcg
     8941 gcagcgtttg ggggagtaga aatttcgttt gtattgcgga gtttgtgtgg gaaaccccag
     9001 tcgacctgat gttataggcc ggctggtagg gtatttgacg gtttgcatat aactgtctgg
     9061 ttcattgggc tggcagctca atacaagatt taggactcat aggaaacaca tgggcttcgt
     9121 taatttcatc atcgaactgc tgaaggatcc gcgtgccgcg attgccggtt ggattgccat
     9181 gggcgtggcg ccgaccctcg gattcatctt cctcatcgta ttcatcgaaa cgggcgtggt
     9241 gttcttcccg ttcctgcccg gtgattcgct gttgttcgcg gccggcgtgt tcgcagcccc
     9301 ggatgctgcg accggcaagg ccgcgctgcc gctgttctgg ttgctgccgg tggtttggtg
     9361 cgcgccgatt atcggcgacc agtgcaacta cttcatcggt catttctttg gccgccgtat
     9421 tatccaatcc ggcaaggtga aggcgctgac tcctgagcga ctggccaaaa ccgagggcat
     9481 gattgaaaag tggggcccgc tggcnnnnnn nnnnctgtgt tcctgggccg cttcttcccg
     9541 ttcattcgta cgttcatgcc gttcatttcc ggtatttccg gtatgcgttg gagccgtttc
     9601 acgccgttct cagtgctcgg cggcctgtgc tggtccacgt tgttcacgct gctgggctac
     9661 ttcttcggca acattccggc cgtgcaggat cacttcgaac tggtgatcgt gctgattctg
     9721 ctggtttcgc tcgccccgac catcatcggt ctgctcaagg ccaagttcgg caagaagaag
     9781 ccggccaagg tgaccgatgc tcagttggac gtcatggaag acggcatcaa gaacctcgat
     9841 aagtaagcaa ggaatgtaga ttaccgcata aggaatcgtc gtgaaaatga aggccctgac
     9901 tgtgcatatg aggtcagggc cttttctgca tgagatgggc gtgaaacaac agcgtggtta
     9961 tccacatttg tcgtggacga catgccgggg tgtggataac tcagcaagct agaaccacaa
    10021 tggtcgattc gtgttgagca ttgaactcgg acacgtttca atcggcttat gaatagcaac
    10081 accacacccc gcgtggcgcg taggatcacc tcgccagcgg tagcacctcc gggcattccg
    10141 ctgcccacgg acgatgcgcc gattccagcg catcgctact acactccgcc gcccaggccg
    10201 ttggcctcat gcgaacggca gagatcgggc gaggctccgg cactcgccct aactacagac
    10261 accagcttcc ataacattgt gacgatgacg tcaggtcatg gaggcgtggg gttgagtgtc
    10321 atggcttcca tgcttgcatg gacattggcg agacgtgaac acagctgcgc tctcatagac
    10381 gctgatttcg tggccggatg ccttgacctg ctgctgggtg tagaacgaga gccgggttta
    10441 cgattcagcc aagtcgacgc acccctcggc cgcatcgagg gcgatgcgat gaaccatgag
    10501 ctgatgatgt gggagggtgt gcgagtactt ccctatgacc cttggagcgc gcgccagcct
    10561 gactggtggg aagttcaagc ggccattcga gccttggcgg aaacgaacga cgttgtcatc
    10621 gtagatgccg gtcagggtgg actcattgag accgtacccg atctgcgcag tggcatgcag
    10681 gtgattgccg cggaattgtc ggtgatgggg ctggccagag ccaagtcgca ccggtccaga
    10741 ctggattcat ggggctgtga agcgccacat atcgtcggcg tggaaccacg tggcgctcca
    10801 cgtgggagag gccacgtggg tattggtgag gcgcaggact atttgactgc cacggtattg
    10861 gggcctgtca aaccaagtgt caatctgtgt ggagacgtac tggaaggcct cggcatcaga
    10921 tcggtgacca aaggcagccg caaagccgtg agcttgcttg ccgatcttgt cgagcaggcc
    10981 attcgcccgg tttcgggagc ttcttgcaag gaccggtgat gcggtatggc tgccgtaatc
    11041 actcgggctg atgagcccgg cgcagtgccc agaactgtgt tcttcggtct attgaatgat
    11101 gtggcatccg acccgcaggt taccgatctg gcggtgacct gtgatggccg cgtttgggcg
    11161 gaccgcggca acggcatgcg agaaacagta ttgcgtgtgc cgtttcgctc gccgcaagta
    11221 gttcgagaat acgccgtcca actgtgcgcg cagcttgggc gcagactgga tgatgcctgc
    11281 ccgatagcgg acgccgccag tccagacggc atccgcgtgc atgcggtgat tgccccattg
    11341 gtgccacaag gtgccgcaat ctccatacgg ttccctgaca ggaccatggc atcgttgcga
    11401 catctgagcc aattaggtgt attcccggct gcttggctgc cgatgttcgt gggactggtg
    11461 cgcagacatg ccacagtgct gattaccggc ggaacaggcg tgggcaagac caccttgctc
    11521 aaggcgctgc tggcgcaatg cgatgtgaac gagcgcatcg tcaccgtaga ggaagttcgt
    11581 gaactgggag aactggggcg tggcgatcat gtgtcgttgg taacgcgcga ggcgaatgtg
    11641 gagggcgtcg gagccataag tctgacggac ttggtcaaag cgacgttgcg aatgcgcccc
    11701 gaccgcgtgg tgctgggcga atgccgtggc gcggaaatcg tcgacctatt gcgcgcgttc
    11761 aattctggtc atcacggtgg catgaccact gtgcatgcgg acggcgtgaa ccgagtaccg
    11821 tccaggctga tttcgcttgg attactcgcc ggagtcagtc cacaggcgtt ggccatgctg
    11881 gcggcaggcg ctttcgacgc ggtggtgcat ttggagcgag tgaacggaca gcggcgtatc
    11941 acgcagatcg gcgaactgcg tgtagcacat ggtgaactgg caggaacacc gctggcggta
    12001 tggaatggct gccatcctcc tcggtatgcc gccgaatggg ggcacttcat tgaacgatgg
    12061 ggcatcgttc ccgggccgag tggtggtggg tgatgatgtg gtgtgctgtt ctggtggcgc
    12121 tcgccgtgct gatatggccg gggaacaagt ccgggaggct gtatcggctg gcttaccgcg
    12181 ggcgtgcggt cgattccgca gccataaacg aacctcgata tgccggctca cctcgcgtgg
    12241 gcgttgcgca ggtgattacg tccgccatcg cgcgattgcg aggtggcggc acattggttg
    12301 aagcattcga ggaacaatcc agcagacgct tcgccactca gcgcattact gtggaacggc
    12361 tcataacggt gttcgaacag cggcgtctgc ccgacgaatc accagcgcag gtacttgaag
    12421 ctgcacgcgg ggtcaccgcc gcagcggttt tgagcgatga gctcggttgc caagcagtgc
    12481 catgtctgga agcggtattg actgcttacc ggcagatgag gctgatgcag aacctgcgat
    12541 cgcaagcatg tgccgttcct aaagccactg tggggctgct gagcgcgctt ccggccgtca
    12601 ctgtggcatt gggcgagctt ctgggagcta gaccattggc gttcttgctt ggatcgccac
    12661 gcggtgtggt ctgcttggta ttagggggat gttgttatgc tgccggcttg gcatggatgc
    12721 gggcattgtt gaaaggcagc ggattatgag tacggtgtgg gtacacgccg cggcaggctg
    12781 tgcggcgatc gctagttggc tgtataaggc tccgtcttgt catgacagcc gcttgccggg
    12841 cgaggaaccg gccgtagcgc atgattgcgg gtcgtcatgg accatactca tattgcgtat
    12901 gtcgcaggtg tccttgcgtc agggctcctc gattccccgg gcgcttgaca tggtgggcaa
    12961 agccgtcggc ggcgactgcg gcgctcgcat gagcgaggtc gggagatccc tgaccagagg
    13021 ggttccctgg gctgatgcgt ggcatgccgc tatggctgtt cagcatgagg gtgcgccagc
    13081 tgatagttca tccggaaaac gtaccagcag aacgcaacga gatcttggcc tgctgcaatc
    13141 ggctttggaa tcgtcatgga cgcatggcga tatgcctggc gtgcgcctcg aatccgccat
    13201 tgaacagctg gaccgtgacg agcgtgccgc catcgaacgg aatgccgcga agctgtcggt
    13261 aaagctactg atgcctacag ggctatgttt tctgccggca ttcgtgctgg taggagtcat
    13321 tccggcgatt gcgtccttca tgatgtgaat ataggcgtga actgagtgcg cttagggtga
    13381 atcttgttgt attgctcggt attgcacttg gctgcgtttg gttatccaca ttggtggtgg
    13441 acgacacgtc ggggtgtgga taactcggca agctagaacc acataccccg ttttccctgg
    13501 tgcatgaagc gttgatagtc cacagtggta tgcaccccgc gtcaacccgg cgcgggggcc
    13561 aaccactcac acacgcatgg ggaaggtgcg aagccactgc gcagcttccc ctccttaccg
    13621 aaaggaacgc caatgaatac ccctatcccc gtcatcgtcg acgggcaggc cggcatcttt
    13681 gcgcacagca aggctcgtgt ggccacgctg aacgccaagg cgaaagaacg gctatgcctc
    13741 atggacgcac gcatccgcac cctcatggct gagcccgaag aaggcgcggc cactgcggaa
    13801 tacgcagtcg tactcgttgc ggctacaggc tttgcggcgg tgctggtcgc catcctgaag
    13861 tcggatgcca tcaagacgtt gctgaccaac atcatcaaac aggctctgaa agtcgggtga
    13921 cagccatgaa gaccatgaca tgccggtggc ggctcgccag actgccgcca gacctatgca
    13981 cgggatcggc caccgccgaa ttcgcaatcg tattgcccag cattatcgcc atcgccgggc
    14041 tgatattggc aataggacgt gtggtcatcg tttccatgga ctgtcaaagt gccgcggcag
    14101 cggctgcacg cgagtttgtg gttaccggtg acgaatcatc cgcgcgcagc atagctgcgg
    14161 acgttgccgg gggagagccc cacgtcagca ttgcccacga cggccaatcg acgagtgtga
    14221 cggtcgaatg ttcggtattg cccggtccgc tggatgtaac tccaacgcgg gttaccggca
    14281 acgctacggc gataagccaa taacggtcgg ctggctgggc ggccgcaaca gaaaggaact
    14341 gcggtgcagg gaatgcgtta tgaggaggga tcgggcacga tggccggagc catgctggtt
    14401 atggtggtgg gcattgcgct cgcggtggcg gcaagtgtgg gcaacctcat gatctgccag
    14461 aaccgagcgc gttccctcgc cgaccttatc gcctttgacg ccgcgtatgc gctgtggcat
    14521 gcggatatcg gtgatccatg tgcgctcgcc gccaacatgg ctgaagcgaa tggtgtagtt
    14581 ttgggctcct gctccgtgcg ggatgaagac gtgttggtaa ccatcaaggt ggaaaccatg
    14641 gtcctggtgg catcatccgt ggagcggatg gctcgggccg gaccggtgag ttgtacggaa
    14701 ggagacaatt atcacaataa tggaccgtaa agcaccgata ctctggcggg cttctgggcc
    14761 tttggctaag gtgagagcat gagcgagagt gaacgcaacg agcgccgtcg cgtggtggcc
    14821 atggtgggca accccacttc cgataaaggc cgtggcgcta aggtggacgc gcaggtattg
    14881 ggtctgctgg aggaagccgg ccgtgcccat aacttcgacg tcatcgacat caccggcacg
    14941 agtttcgacg attccctgaa ccaggcgcgt gaacgcgcgc acgagtacga ctatctcgta
    15001 gtggtcggtg gcgacggcat gatcgcactt ggagcgaacg ccgtgggctg cagtggcaag
    15061 ccgttgggca tcgtggccac ggggtcgggc aacgatttcg cacgaggact gaagctgccg
    15121 gtcaaccgta tcgaaaccgc agtggaaggt atcgtcggtg ctatcgtgcg cggatcacac
    15181 atcgatgtgg acatgggacg cgtgacctct ttggacggcg gctatgccgt ggatccctcc
    15241 actggcgagg agtacgagta ccaggagagc aagtccgccg agcatccgat tgaccggtac
    15301 tatgcgggca tgctgtcctg tggtcttgac gcgagcatca acgaccgtgc gaatcattcg
    15361 catctgccga ccggcaccat gcgctacttt gcggcggtgc tggtggagct gacgcatatg
    15421 aagcgttatg gttaccacat caaagccaca ttggcggacg ggaccacgga tgaacgagac
    15481 atcatcacac cattgctgac gattgcgaat tcgaggcata tcggtggcgg cattgaggtc
    15541 tcgccgtact cctgcttctc cgatggattg ttggacctgg tgtggatgga ccacgtgccg
    15601 aacttcggcg aatgcgcggt ggccatttcg aatgcctata acggcaagct acttgcttca
    15661 aaggtgttcg gctggaaacg tatccgtgaa atcgaagtca cgcgggcaac ggaaggagac
    15721 gagccgccgg tgctgatggc ggacggcgaa tatatcgggc atttgccatt tagggtagtg
    15781 gccgaggact gcgcgttgcg cgtgctagtg ccgccggccg tagccgcccg tgaggtggat
    15841 tcccgacagg aagtgctcaa cgccattgcc cgagatggtc gcgatcccgt gaccggccag
    15901 ttcgcgtgac ccgattatat tggctcccct cattaggctg agccgtaaag caaacagccc
    15961 agtgggctgt ttgtaggcga gggttcgacg ataatcgaac cagaagaaag tcagccgtag
    16021 gctgtccgca attgcggacg agctggccgc gaagcggact gaggggagtg cgggagagcg
    16081 gtaccagagc caatggccgt cagacctcag acgacatagc tttcgatatg cagcgtctgc
    16141 gccatgacct ctttgatggt tcggtcgctg atgttcggat gcgagcgaat cagcgagata
    16201 agccccacaa tcagtgtgaa gaacgtctca tggacgttgg tgattggcag gccatgcatc
    16261 tgctcgaaat cacgtacggt ggtctgggca atgtagtcgg caatacggtc cgccgcgcgg
    16321 tcaatgaact tgatatacag ttcggcgttg ccgtcttgga tcatacggtt tgagaagggg
    16381 ctctcgtcgg cgatcaggga gcggagcaca tgcacgatgt cgtctaatgc cttgttcacg
    16441 ttgcctgttt cgcgcgcttg gttccactgc ttcaacgtgg tgaggatttc gtcgatgacg
    16501 ttatcgagta ctgcgtcagc tacggcttcc ttgtcttcga agtagtggta gaacagcgaa
    16561 cgcgtcatac cgacttcgct tgcgatgtcg gaaacggtga ttttggagaa gcctttttcc
    16621 agacacacct tgcgcgcggc ttgcacgatg gcgtcgcgcc gggcatcgcc gcgggtgggg
    16681 cgtttctcgt cgagggtatc gctcatgggt gcctcctgac cacaaccgtt attgacacta
    16741 tgttaataca aagaaagcgg ttaataacaa taaaaatgcg gtaaccgttg tcaggtgaat
    16801 gtcgttggaa tataaagagt tttccgattc ggcaactatg agcccggttt atcttgactt
    16861 tttatcggtt aattctgagg gcaaaggtag gttagtagct atggcacttg cactgtatcg
    16921 tcgctatcgt ccggacacct tcgaaggggt catcggccag gaccaagtca cggtcccgct
    16981 gatgcgcgcc ttggacgagg gcaagctcac acatgcctat ctgttctcag ggccgcgagg
    17041 ctgcggcaaa accagctccg cgcgtatcct ggcccgttgc gtcaactgtg ccaaagggcc
    17101 gacttcccac ccctgcggcg aatgcgaaag ctgcaaggac ctggccaccg gcggtccggg
    17161 ttccatcgac gtggtcgaaa tcgatgcggc ctcgcacaac ggtgtggatg atgctcgaga
    17221 attgcgagaa cgcgctggtt tcgcccctgc gcgcgaccgc tataagattt tcattctcga
    17281 tgaggcccac atggtcacgc agcagggctt caatgcgctg cttaaaatcg ttgaagagcc
    17341 gcctgaacat gtgatgttca tcttcgccac caccgagcca gacaaggtga tcggcaccat
    17401 ccgttcgcgc acccaccact atccgttccg tctggtgccg caggaagtca tgggtcccta
    17461 cctggagacg atctgcgaca aggaaggcat caagcccgag cccggtgtgc tgaaactggc
    17521 gatgcgcgcc ggtggcggct ccatgcgaga taccctgtcc gtgcttgacc agctgatggt
    17581 tggttcggtc gaaggcgtta tcacccatga tgccgctgta gccctgctcg gcttcacgcc
    17641 ggaggcactg atcggtgagg ccgtggatgc ggtaatcgat cacaacggcg aagccctgta
    17701 cggcgttatc cagaaagtcg tggtcggcgg attcgatccg agacgattcg tcgaagacct
    17761 actggctcgt gtgcgtgatc tgctggtgct tacgttggcc ggggaccgtg cggaatccgt
    17821 gctgtccgac acggctgagg ccgaggatat ggacgatctg caccgccaag ccaaggcgct
    17881 gggactgggc gcactgaccg caatggcaga catcatcaac actacgttgg gtgccatgac
    17941 cggagccatt tctccgcgta tgcgcttgga actgctggcc gcgcgattgt tggccggcag
    18001 tgaatcaggc tttgcaacag ccgctcccgc gccagcttct tctggtatgc cgcctgcggc
    18061 tgcttcttcg acttcgacta cgtcagccgc ttccggtacg ggtgccagtg gttttgccgg
    18121 cgcatcgcgg ggcgggtttg ccggagcctc acatggcgga ttctccggtg ctgcacgcaa
    18181 ccagcaagca gccgcgcctc agagcaccgc ttcccatgaa tctgttggag gcaacggccc
    18241 ggtcagcgac cgacctgcgg actctccgtc tgtgcaacct gttgtagcaa ctgctgctgc
    18301 cggtgccggg tggggcgctc ccgccgcgtc gccggccgtc ccggctcaac cggtgacatc
    18361 gcctgcggca tcggataatc ggtccatcga cgagaagtgg gatgcagccg tcgccgcttt
    18421 gccggaaacc attcgtgaat atgtgtcacg agacaaggtg ccgaccgtaa aattcggccc
    18481 caaccgtaag ggtctgcctt gcctgtcgat gacgttcgac aagtcgctga gccagcacgc
    18541 tttcgccttg gccgtggata acagcggtaa gaaggcggcg gccgtggtga tggatgccgt
    18601 gcgcaacgag ttcggcgcca atgcggttat tgcaccttct gcggtcgcgg cgaatggcga
    18661 gcgagtcgaa tccgtcaaac gtatgagccc ggagcaattg gccaaggtca agcagcagat
    18721 tgccatggcc aaggctggat tggccgcgtc gagtttgggc gcgagcctgg gcattcatat
    18781 ggggagcgag acgaaagctc cgaagccgac tgcaacaggc caagccgagg acgcagacga
    18841 cagccatcga gccggacaat ccagtgcatc cacggcagcg aagaactgga gtgacgatga
    18901 cccttgggcc aagccggccg taagcaacgc ttcgccgcaa tctgcagacg ggttcgcgcc
    18961 taacgagccc gctgctacgc ctgcgcccga agagcatcac aagaagcatg ttgcggttcc
    19021 ggacatcagt gacggtgttg atccgtgggc cgctccggct gctcctgttg cacccactgc
    19081 gtccgttgcg tccgctggac ccactggaca gtccactggg cagccagccg ccgcatcttc
    19141 tgctacggca gttgccggcg agagcgacga tccatggaat cagacgcagt ctcaggccgc
    19201 ggtctcaccg gcgtcgcaac cggccgaagc cgacccgtgg aaccagccat atcagcagtc
    19261 tcctcacgat gatccgtgga atcagccgca ggggaaccat gctcaggcgg ctccacagcc
    19321 aggagacgac ccgtggaatc agcctcagcc ccagactccg gcttcttcgg ttcctcagac
    19381 agtgtcgggc aatatgtcgg gcaatgaccc gtggaaccag ccccaatcgg ttccgcagcc
    19441 g
//
LOCUS       DAWBMW010000017        17510 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_851_length_17510_cov_3.399885, whole genome shotgun sequence.
ACCESSION   DAWBMW010000017 DAWBMW010000000
VERSION     DAWBMW010000017.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 17510)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 17510)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17510
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_851_length_17510_cov_3.399885"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2812..2821
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6783..6792
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13483..13492
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3302 a         5037 c         5349 g         3792 t
ORIGIN      
        1 tccgcatggt ctaagccata cggaggatca actgttttac ggacacccgt ctattgatca
       61 agcggcgttt tttcgtatag ctccaatcag atgaaagcgc gctcgccgaa gatggcggtg
      121 ccgacgcgca cgatggtgga gccttcttcg atggcgtagg ccatgtcgtg ggtcatgccc
      181 attgacagtt cggtgcagtc ggccgtgcct tcggcaccgg aggcaagaat ctggtcacga
      241 gtgcggcgca gatgcgcgaa gcccgcacga atcgtgcgct catcatccac atgggcacca
      301 atagtcatca atccctgcaa gtgcagtccg cccatcgcac cgatgcgctg agccaagtcg
      361 atggcgtggc tgggtgcgca gccggacttc gattcctcgc cggattcgtt cacctccagc
      421 aacacgccga ccgtgatgcc gcgcatggtg gcgcggcggg cgatcttctc cgccagttcg
      481 atggaatcca cggattcgat ggtgtccacc gccggcagca ccttgccgat tttattggct
      541 tgcaattggc cgatgagatg gaaaggaata tgcgttgcag ctgcggcatt ggccgtgtca
      601 gcctcagcgg catcaatcac gcctagcgaa taaccacgct cccccagtcg ttttgccaga
      661 ccgtcagcct tgacagtaat ctcctgtgga cgattctcac caatcacgcg aataccagca
      721 tcgatagccg ccagaatctc gccgacatcg cgcgtcttgg tggcagccag caattggacc
      781 gaaccggcag cacgcccggc tgcggactcc gctgaggcaa tccggtcaag cacgcgatgc
      841 acaccatcag taatctcttg tgcgcgcgcc tgatcgataa cttcgttggc gagatcctta
      901 tggtccatgt atgccgtcat tgctgcccct gttcgtagat tccccaccat cgtagtctca
      961 tacgcgcaca taatgcttgt tattgaacaa aagtgaagcc cccgctgttc atgcgcgggg
     1021 gcttggtgat gctaggccga gcgagtggga ctagcaggta ccggacttgc ggcgcttgag
     1081 gatgattaca cccgcaacag caagtaccac ggcgaggatg ccaacaccaa gcacggctgc
     1141 accggtgtca ggtatcttct tgtgcttgcc ggagtctgca tcacccttgt tctggttgga
     1201 atcgttgcct tggttgggct tgtccgtgcc ggaaccgggc ttatcgccac cctggccagg
     1261 cttcgtggtg ctcttctcaa ccagacccgc gatggcatcg ctgattgcct cagtcgcagc
     1321 ctgtacgtca gactgggcgg cctgaccatc actcgtcagc gcattcgccg tagcgactgc
     1381 cgaatcaagc acggccagcg attcatcagt ccactttgtg cgatccacgg cagcagcggc
     1441 atcgagctga gcgctcagcg cggcacgatc ttgcaccgta gctacaagca ccagcttctt
     1501 cgctgcctgc ttcagctggg ttgcggcatc caccaccgca gcttcggtgg catcctcagc
     1561 cttatacacc tgctgcgccg aatccaatac cgccgcgtac tccgcccatg aagtctcggt
     1621 gtacttggag gcatcgtcag ccttaggcga gtcagcgagc gcctgcttga gctcggtctt
     1681 gtcgatcggt gtgggctcag gcggctcagg cgtactgtcg tcaatggcat gaacctgcca
     1741 ttcggcaaca gccggagaag ccgagccgga cgtgataacc atacgcaact tggtagtctt
     1801 gaccggagtg ttccacgtgc catcagacca ccccttggca tcgccggtgg cccagccagc
     1861 agacggtgaa ttggcggtca cagtgtaggt ggcgtcggaa atatccacga agttgccggc
     1921 accgttgttc gccaaggcgt cccagtactg caattcccac gatgccggaa tacccaatcc
     1981 gccatcatcc gaggtgaatt gcacgcggct agagtcgata gtcacttcac ggtcccactc
     2041 gtagtcgagg cgcatctcac cggtgttggg ccaagtgccc cagacatcct cgttggcaat
     2101 ggtgatatcg ggaatgatct tgccgtcgtt caacgcggcg gcacccttga cccagccgga
     2161 tgccttgccg tccacatcgg tggcggtggc atcgccctca atgtagccgg tcagcggcac
     2221 ggaagtgtcg gatggaatgt cggttttcgc cgaagcggac acgttgccgg ccttatcggt
     2281 ggcgcggtag tacacggtgg cgcgcgacgc gctcggtgca gcaatcggct tgctgtacgt
     2341 ggcccattga ccgtctgtgc caatgcggta ttcaattgat gcgacaccgg aaccggtgcc
     2401 gtcatcagcg gtcaaggtca gcgtgcgctc ctccttatcg gcggcggcgg tcacggttgg
     2461 cgcggcggag tccaccttgc cgccggcctt ggccacgtcg gaaacgttgc cggcctgatc
     2521 ggtggcgcga gcccacactt cgacatcgcc ctgctgggaa agcgtggcaa tggccgcgtt
     2581 tgcattcgcg gcgatggtgg tccagctctt gccttgatca gcggaaatct ccagcttggc
     2641 gataggtgcg gtgtcgtccg tggcctgcac ggtggcgaac ggagcggtgg tgagccactc
     2701 gctattgcct gcgttagcag taacggcaac ggttacagtc ggcttcgtgg tatcagcggc
     2761 aggctggtag ccgtcctgca cggtgacatt taccgacaca ttgccctgct cnnnnnnnnn
     2821 ncgtaggccg taaacgaacc gaccttggca tacgaggaag catcaactgg acgccaaatt
     2881 actggggagg caacggttgg gttcccgtac ttcacatcca ctgtggccgg cagagtaggc
     2941 gcagtaccgg tggcggtggt cacgctcttc ggcgcagtga ttgccggcga ttcgctggag
     3001 tcggagccga cttcccattc agcaacggcc acggaggcat agccgccatc cacagcttga
     3061 ccggtcatgt cgagctttaa ggcagtagtg gtcaccgcat cgaaggtgac ggcgttgggg
     3121 ttggccttgc ctgtggtcgt tgtataggca ctcgggttgg tgacatcctt ccatgtgccg
     3181 tccgcgtcca agtactgaat cttccacgag gcgggcgcgc gcagagcgtt ggaatccacg
     3241 tcgaaccaaa ccttagacga gttgacgcgc acagtgccat cccattggta gaccagagaa
     3301 tcgccggact gcgattgcgg gatagaacgg tagttggtcc agtgattcgg cgggaccaca
     3361 gcggtttgca acacacggcc gtcgttcacc gaacccttcc aagcaggcgt ttcagtgaac
     3421 gtggcgctca cctttgcgta cggagccacg ttggtggagg gctgcgtttt ctcggccttg
     3481 gttggatggg cggtggaaac catctggccg tcggcggtcc aatcgacttc atcaatgcac
     3541 acgacacggc ggaaatcggt gccgccttcc ttgtcgccgg tgtgataggt cacataccat
     3601 ttgtcgccga actggagcac cgaggggtgc atcgtggtgg ccgagccaga aggcacgatt
     3661 acgccttggt acttccatgg gccgagcggg ctgtcggaag tggagtactg gatgcaagct
     3721 tgatagttcg attccacgca ggtggccgtg cccgaaccgg gcttaccgcc gtcgtacacg
     3781 ttgtaatagg tatcgccctt acggaacacc cacgccgcct cgaagaatcc cttcgggccg
     3841 ttcgcgttgc tgtccgcgtc cttcatggtg tggaggttcg gcgtggtgcc atcagccttg
     3901 gtttccacct cggtgtatga ggtgcggccg gttgtggcgt ccttcttcat cttgatggcg
     3961 agctgagtgc cgaacgtacc aaagtgcaaa tatccggtgc catcctcgtc cacaaagaat
     4021 gccggatcga tggtttcaat ctgggtttgc gatgtggtga gcagcggctt gccgatggca
     4081 tccttccacg ggccgagcgg actcttcgac ttggccacgc cgatggcgaa cgggtcctgt
     4141 ccagccgcct cagaagcctt gtccttcacc ggcacgtagt agtagaacca atcatcggaa
     4201 gtgtcgctcg gggtgccgtt atcgtcggct acgacttggc cggcgtacgc gccatcgccg
     4261 gtggcccagc tgaacaggtc tgccttggcg atggtcttgt aatgtgtcca tttgccttga
     4321 ttgagaccat cttcatcggt gacgtatacg ccccagtcct tcatgttgaa ggagccgtag
     4381 tcgggcgaag cctcatcgtg accggtgaag acgaagagtt tgccgtcata gctcagcgcg
     4441 gatgcatctg cagagtagta gttctgcgaa agatcgttcg gggtgtcaat accctcgttc
     4501 tgcccgatgg ggttgcgcat gtagctgggc agtgcgctat tgtcgatttg ggtgacggtg
     4561 acatcaggat tgttatacgg cagttcgtta tacgcgtgac cggcaacgct gggcgcgttg
     4621 tcagcggtgg ctgaatcggc ggccattgcg gtcggcgcaa gggcaaggcc tgacagcaat
     4681 gtcgctgcgg cgacgagtcc accggccacc gcgcgaaccg attgcgcgga tacatggaaa
     4741 ctcatagtgc tcctcattga gtgagtgaaa agagtaatga tgggtagaag gaaaaggcta
     4801 gagccccctc tgtgaggggg ctcactcggc agaatgactc cgttgcgggt caggccgagt
     4861 ggcgagccga accgtggtcg ccggactggc gacgcttcag gatggtcacg cctgcggcgg
     4921 tgagcagcac ggcagccgca gcaactacgg aaaccgcggc accagtcgga ggcacacgat
     4981 cacccttgtt gtccttatca tccttatcag tgtcggtcac accaggctgc tgggagccgg
     5041 gcttctgctc aaccttggtg gcccaaaatg ccttgtcaag agcatcctgt gcggcatcga
     5101 cgtcggcctg cttggcgtcc ttgtcgtcgt acaccttctg agcggcggca atctggctct
     5161 gcagagcctg ccatgccttc tcggtgtact tcgacttgtc agccttcttg gcctgctcga
     5221 tggaagcctt gagagactcc ttcttcaccg tttcgacggc gggcttctct tccaaggcct
     5281 tgacggcctt gttcagcgaa gcggacgctt cctggacggc ggcctgagtg acctcaccgg
     5341 actcatcgcc cttgagcaca ccttccgcag cctcgatggc cttgttcaag gcctcgacac
     5401 tcttatccgt gtacttgccg gaagcggcct cggccttggc cgtgtcgatg gccgcctgca
     5461 acgtggactt ctccacgtcg gtggcgagcg caacgagcac cttctggccg tcctcgagct
     5521 tggtcacctg ctcggcgacc tgttcttcgg tggcattgac atcgtcgtac acagcctggg
     5581 cagcgtcaat gatttgctgg aactcagccc atgaagcggc ggtgtagcgg cgtgcatcga
     5641 ggccgttggc tgtgtcaaga gccgacttga gcgcggtctt gtcgaccggg gtaggttcag
     5701 gctcggtgcc gtcaatacca tgcacgcccc actcggccac ggcggccccg aaggtgctgc
     5761 tgccggtgta cggagtgagc acgagtcgca gcgacttggt ggtgaccggc ttgaaggtca
     5821 cagcgctcca acccttggca tcggtatccg cacgagaagc cggcgaatta cgcacgacgg
     5881 tgtaatcctc ggtgggctcg acgtccttcc aagaaccatc ctcggcgagg tactgaatct
     5941 tccaagcgtc cgggacttcc agaccaccct tcgaatcgtc agtctcagcg aagttggccc
     6001 agaactgggc gcggctctgg tcaatggtca cggactgacc gaagtcgtac tgggcgtaca
     6061 tgccgtcctt tgccttgccc caagaacccc agaccttgtc gttgacgttc tgatcatccg
     6121 tggtcggcca agtatcatcg acgactacgc cgtcgttgag cttgccttcg gccggtgacc
     6181 aggtcgcctc ggaatcaggc gaaatggtgg tcctggtcgc cttgccttca atccaaccga
     6241 ccaacggctc aggctcaggc tccggagtgg aggaaccgtt cgggtcggca accacgtgga
     6301 cagtgagctt ggcagctgcg gaagatgaac cgtcgtcgac ggtaccagcc acctcgtagt
     6361 caccaacctt ggtcaggtcg atggccttga tgtcggcatc cttccacgtg accttgcggt
     6421 cgtcgttgta tccgttcggg aacttcaccg gaaccgtggc gggcaggtcg atggacttgg
     6481 cgttctggta gacggtctgc tcaaccggct gcgcattacc cacggtctgc gatgcggtat
     6541 cggaacgagc cgcgatgtgt gcaaccgtac cgttcagcgc accggcgacg gtgcccttga
     6601 catcgaatac ggcatcggcg gccagctgag cgtcggtcac cttaccccac gtgacggcga
     6661 ggtcacggcg agaaccgtcg gtgtatacgg cgctcacggt ggagggcagc ttcagatcat
     6721 cggccttgga accggtctgc accagtcggt tgacttcgtc cacgctcacc ggctcgatgg
     6781 cgnnnnnnnn nngaactcgg aagcacccac gatggcgccc ttcgggaaga ccacgcgcag
     6841 cttgttggtt tcgacggtgt cggcgaactt cacttcgttg ccttcgttcc ggttgaccgt
     6901 gtaggactgg ccgtcagcca gtttcacatc ctgccagtta ccgtcagcgt cagcgtactg
     6961 aagcttccaa gatgagacca tcggcacgcc accgccatcg gtccagaaga agaccttggc
     7021 cttcttcagc ggcaccttgc cggtccactt cagttgcagc cacggctcca caccggtgtt
     7081 gtcgccccag ttgttccagg tctcgttcgg cgtgttggcg tataccacct gaccgtcgat
     7141 gaccttcttg gcgttgtcct tcggctggta gccgttggtg tagctggcag aggcgctcga
     7201 ctgcggggct acgttcacgg taccgtcaga ggggatgccg tgaacggtga tttccttgga
     7261 gccttccttc tcgccatcgg aagcggtgag cttcaggacg tagtcaccct ccttgttgaa
     7321 ggtgacggtg gtgctggcag cggaggcgtc cacgaacttc gcagcaccgc cttccggacc
     7381 ggagacctgg gaccatgtgg tggtgacgtc gccttctgcg ggcagaccgt catccttcac
     7441 cgtaccgacg agcttggcac cggatgaggt gctggaagac acgtaagcat ccacctgcgg
     7501 agtctggttc tcagaagtgc cgtcagcctt gataccggtg ttgtaggctt cgatctcctt
     7561 gacaccgacg gccatgccgg cctgcggcgt gaaggtgacg cggatggtcg tggtctccac
     7621 cggagtgaac tggatacggt tgtagttcgc gcccgcgtag ttcggggtgc gcacctgatc
     7681 cgcaatcggg gcccatgtgc cgtcatcctt ctggtactcc aacttgtagt tggcgggctc
     7741 ggcatagccg gagatggtct ggctggacga gctctggtag aagtacaagc ggatgtcgtc
     7801 gatcttctgc ttgccgtcct tgaacttgat attgagcgtg tcggtcttgt tcccggagcc
     7861 cttggtaccc cagaacgatt ccatcacggt cttgccgtcc accgcgttct tggccgggta
     7921 gttggtgtcc ttctcggcgt aggtggtacc ggtcacgtcc gcgtccttgg ccacgttcgt
     7981 ggtggacttg gaggcggagt cgacgttggt gccggacttg gcgaacaggt cggtcacgcg
     8041 ctgatcggca ttgaaggtaa cctgattggc ggccttcacg ttcttgacgg cttcggcgcc
     8101 gacggtaacc tgggcaccgg aatcgtcctt gacctcgacc gttcccgcag ccgggtcata
     8161 gatgaggtgc gccagcttgt cggaggtgaa ggcgagcttg ccgcccacgt acaggctgta
     8221 gccagcagga ccaccgtagt ggctgccgtc cttgtcccat acgatggaga catcctgatc
     8281 gtggtagcgc aggttgttta ccgtgaagta gttccaaccg gggatttcga tcgggtttag
     8341 ctcaatcttg ttgtcctcgc gcggcaccat accggctacg tcctcgacca tcgtccagtt
     8401 cgtggtgccg agctgggtgt ggtggatcca ggagcggtag gtgatcttgc cgccgttctg
     8461 tgcggcgtca aggttcttgt cgaggttgtt gatcacgccg tcaccgttca catcgccgac
     8521 gttgttgttg tcctcgttcc agaactcgtt ctgatcaggg tggttgttat cgccgccctg
     8581 gtagtgcgcg aacgccaccc agtacagcag cttcttgaac tgctcattgg tgatgtagcc
     8641 gttcttggct gcatcgtagt tgcgaatacc ggccgagtag acctgcagca gcggctgcgc
     8701 attaatgatg gagaagttgt tggaaccggg gaagttcaac gcggccttgt ccgcctggtt
     8761 ggcggtgaag aacgggaaga tcgggaactc gttggaatcc tcgaacaggc gcagagcctt
     8821 gttgtaatct tcgttgccgg taggcatcag gccttcggag tacgggtagt agttattgat
     8881 ctccttgtac ttggcgaaag caccgtcgtt cagccacctg tgcttgagca ggttgtcgga
     8941 cttgtcccac agttcagtgg tgacttcttt ctggatcttg tcggcgatgg cccgcatctt
     9001 ctcggcgttg gcggtatcgc cagcagcctt gtatgcctga gcagccgcgt tagcgttggc
     9061 ccacatattg gcggaaccat cggcgcgatc catgcgctgg ccggagcgac ccggctcaga
     9121 gaaggaaacg gcgtcggcat cgttaccggt catcgaccac caggaccagt caatcaagct
     9181 gttgtcattg ttgttctggt tgtcgttgtt gtccggctcg ctcttggaag cgagtagacc
     9241 ttgcacgtcc tcggcaccct ggtcggccag cttctcggcc acggtggacg gaccgccgtg
     9301 caccttgtag gagtcccagc cggcgcgcgt gatgtactgc gtgtagctat ggttccagtt
     9361 ggccgggtcg cccggattgt cgtggtagta gtaatagccc gccttgctct tcttggcggt
     9421 atcgccggcg gaaagccagg tgccgtagga gtactcgggg ttgcggaacc acttggtgtc
     9481 catcatgaac atgccggagg tgagcacgat ctggttgttg tagccgagca caccctcgat
     9541 ggaggtcggg tactggaagg tgttgccagg catgttggcg tcgagcatgt tgaaacggct
     9601 cagccaccag cggtagacca cggtcttatc gatgttgtct tccggagtgt ccacgtaggg
     9661 agcgttatcg acccaccact tgttgtaggt ggtcacggaa tccttgtagg aggcggcagc
     9721 atccttaagg tcgccggtgt aacgggcctc atattccttg gtggagtcag gcagttcgtt
     9781 ggcgatcagg ccgagctgaa tcttgacggt ctgcggttcg ttggccttga ggctcaacgt
     9841 gctggtgagt ttgccaccgc tgacgatcca gttggaaccg tcctggttgt tggcggagaa
     9901 gcgcggatag atggtcgtca ggttgttctt gacgttcacg cggccagtaa gttcggtggc
     9961 cccatcagca ccctcggcgg cgaacggcga ggccgtggtc agtgtgacat cgcggtcctg
    10021 cgtgctttcc acggtgaggt tcgcaaccat cacgttgttg taggtgatgt acttaacttc
    10081 cttgatcttg aggctcttgc caccggtctg gaacaaggag gtgaagtagc tcggcgtctg
    10141 cttgcgctgg gaggcgtctt cgttaagggt ctggtttgca ccgtcagcgt ttacggtaac
    10201 ggtaaagcca tccctgttgg tctcatccca gtaggcggtg ttacccacaa aaccgagccg
    10261 attaggcgtg tgcgtgtaca tgtaggcggc gcgaccacgg gtgaacaggt agttgttgcc
    10321 gtcggagcct tcatacgagt aagcaccctt gtcgttcgtg cctttaccag ttggctgtgc
    10381 accagtacgg gtgagaatct tatcgatcca gaagtcatgg tcggtgtcgg tgccggccag
    10441 gttcttcacg tcgttatcga atgtctcctg cagatagctg gcggcagggt tatatgtggt
    10501 gttgttcgcc gccgtcttca tcggatcatc agaccccttg aacgacggat atgccacatc
    10561 cgcattcttg atgacgggcg catcggctgc ctgcgcggtg actgcaccgc cggtggccag
    10621 tgtggcgacc gctgcaaccg cgccgattga cgcaagccac cgctttcgtg ttgattgatg
    10681 cattcctgct tggacctctc cgccccggtc tttcgcgtct tcgctcaggc tacgtcgcgt
    10741 ctatgcgacc aggacttctt ctgtggatgc tgggccgcag agcccgttct ccatcagttt
    10801 cctttattgg ttattgggtt caccggaatg agtgtcttca gtaaatctgg tgtgatatcc
    10861 gccatggata tcccggttgg agcaattcac cgaagctgat tccacatcat aaccgagcga
    10921 atacggtttc acaactttcc ctcagtgata tcaaccatct tatacaacaa aaacccggta
    10981 atttctcagc tttcgcattt attttcgcat tagggcattg ctgtgtttcg ccgtgtcgca
    11041 gtacctacgg cgcaacgatt cagcctcgct gaatcaggaa ccaacaggct cccgctggcg
    11101 ggagctggct cgcaaagcga gactgagggt ggcttacacg gtaccgctta tgaccaccct
    11161 cagtcgactc cgccgacagc tctcacagcg ggagcatata attaaaaaat ctcctgcacg
    11221 ggtattaccc cgcacaggag acctcattcc ccggatgtgt tttcggctcc aatcgatact
    11281 gacatccggc ctatgccgtc atcgacttat tgcagacgat ggcggcataa gtattttttt
    11341 gccggccagt tgcgcgcagg ccggcatatc gccgatcttt atcgacgctg gaagacacgc
    11401 atctcgccta tctcgcggtt ggcccacgag tagtacggca ccaagcgcag cgtcgcgggt
    11461 tcgcccgcac gcggttcgtc ggcatccacg tacagcggcg cgtcatcctc gtcggcgcgc
    11521 tcgcgcactg ccggcaaatc aacggtatcg acgccaccca gcaagtcggc ctggaaagcc
    11581 acagcggcat ccgcaccggt gacgccatcg gccagacgat agttccacag atcaccgggg
    11641 ttatcggtct gttcggcgca gtagaccagc gggccgcgca tcacggccac ctgaccggca
    11701 tcggagcgca cgcgggagtt ggcgcgcacg aacttcacgg acatgtcgag ctcgagcgca
    11761 atctccaacg tatcgccggc gttgaccaca aggtatacga agccgtcttc cagcgagccc
    11821 actgcgggct tgccgttcac ggtcagcgtg taggagcccc gcgaccagcc ggggatgcgc
    11881 agtccgaaac ggaccgagct gtcagtggcg ctggcgggca ggctcaccgt gtattccaca
    11941 tggccatccc acgggaagtt cgaacgctgc tcgaccgtca ggccggaagc gaattcggct
    12001 gtgttggcga tgaactggtg gctcagcacg gtcttgccgc cgtcgcgctc ggtgtagatg
    12061 tagcggtcca cggaggcgat gagtcgggcg atgttggccg ggcagcaggc gcagccgaac
    12121 cagtcgacgc ggtgggagag cacgtggtgg cggtccgggt tatccagtcc gtcaggcgtg
    12181 gtctccagtg cattgacgta gtagtactgc ttgccgtcga gcgagatgcc ggcaatggaa
    12241 ccgttgaaca gttccttctc cagcacgtcg gcgtattcgc ccttgggctc gaggtcgagc
    12301 atctgctggg cgaacatgct catggccacg gaagcgcagg tctcgccgta catcgtgtcg
    12361 ttcggcaggt cgtaatcgta ggtgaacgat tcgcccacat gggtggagcc gatcgcgccg
    12421 gtgacataca tacgacgggt gacgatgttc ttccagaaac gcttggcagt gtcgatcagc
    12481 ccctgatcgc cgagtagtcg gcccacgtga gccacaccgg tgcacaggta accgacgcgc
    12541 acggcgtggc cgtccgcggt ctgctggtcg cgtacgggct cggcagcctg gaagtaggtg
    12601 ggcttgtaga agccgagatc atggaagatg ttgtcgccgt tcagggcctt cagctgcttg
    12661 gtgtagaact gagggtcttg gccgcgcacg tcgatgaggt attgggagag cgtcaggtaa
    12721 cgcttttcgc cggtttcctc gtacagtttg gcgagggcga gctcgatctc aggatgacca
    12781 tcggcgccgt ggatcttgcc ttcttcgggg ccgaagttgg catccaggca gtcggccatc
    12841 tttttggcga cttcaagggc ctgctcgttg ccggtcacct gatggtaggc gacggcggcc
    12901 tcgatgtagt gacccatcac gtacatctcg tggctctgct gaatcaggct gaagcgcggg
    12961 cggtcggccc atacgccgga cttgatctgg tacggggtgt ccaaatagcc gtccgactgc
    13021 tgggcgcggg cgatgagatc gaccgtgcgg tcgcacagcg ccttgagctc gggatccggg
    13081 tggtatgcca atgcgtaggc ggcttcctca agccacttgt agacgtcgga gtcttggaag
    13141 accatgccat ggaactcgtc gtccaattcg ccggcggcaa ccttcaggtt ggcaaccgca
    13201 tggctcttgt tgtcagccag ctggttgccg gctgggtcgt cgggcaccgt ggtgtcgatc
    13261 tcgtcgttca tcacgcccca ctggtagggg atgacggatt cgacgatctg gtcgcgacgc
    13321 cgcttccaga acggggaagt gattgtaacg ttcatgattg tccttttttt aaggcccttg
    13381 accacaccgc ctccggccct gcaattaagg accggtttca gccgggctca gtattggctt
    13441 cgacgctgtc gcgtctcagc acggagcttg ctgggagccc acnnnnnnnn nnccccagcc
    13501 aagggccggc ggcacgagcc gtcagccctt gacggctccg gccataaagc ccttgacgta
    13561 gtacttctgg agtgcgagga acaggatggc gcagggaatc agcaggatca cgacgccggc
    13621 ctcggtggca ccgtaatcga tgacgcccat ggtctgctga cgcatgttca ccatcgccag
    13681 cggcagcgtg gacttggacg aagagctcaa gtacagcgga atcatgaagt tgttccatgc
    13741 ctcgaggaag gcgagcaggc caaccgtaac catcgaaggc ttgaccaccg gcaccaggat
    13801 cttgaacaag gcctggaggc tgttgcagcc gtcgaccatg gcggcctcct ccatgtcctt
    13861 ggggatcgcg tcaaatgcgt tacgcatgat gaacgtgctc ataggcagct ggaacagggt
    13921 gatgaccagg cccacgccca gcaggctgtc gttcaggccg atctgcttgt accagaccat
    13981 cagcgggatc agcagcgagg cgtacggtac catcaggatc gacagggtga ccatgaagcc
    14041 gaggttgcgg cccgggtagt cgaagcggga gaacgagtag ccggccagcg tacagacgac
    14101 cacggagaac acgaccgcga ccagagtcac gattaccgag ttgaacaggt acttgggcag
    14161 accttcctga taaccgaaca gggtcaggta gttggccacg ccccagccct tggactgggc
    14221 cgaaccggcc tgcgggctga acgagcagat gatgacccag accagcgggc tgatgaagat
    14281 gatggccagc agggcctcga ggatccgggc gatgatacgc ttccacaatg gggtagtcat
    14341 aatgtgttac ctcccctttc tcacttggtg ttgtcgcgca tgccgcgcat ctggatggag
    14401 ttgatgatga ccagggccac cagcacgatc agcgagagcg ccgcagccat acccagatcc
    14461 ttcttggaat cgaaggcgaa gttgtagatg agctgcacca cggtcatggt ggagttgttc
    14521 ggaccaccct tggtcagaat gaagaactga tcgaacgcga ggatcgagcc ggtcacgcag
    14581 tagaccaggc acatcacgat ggtcggcttc atcaacggca gggtgatcga gcggaagata
    14641 cgccaggaac cggcgccgtc catgcgggcc gcctcgtaca gatcaccggg gatggcctgc
    14701 agaccgatca tcatcagcag catgtagtag cccgagaaac gccaaacaat cacgataatc
    14761 gtggcccaca gggccgactg gccggtggcc agcaccgaac caccctcctg catcaggccc
    14821 aacgagctca gaatcttggt gacagggccc acctgcgggg aatacagggc gtagaacagc
    14881 agggacgcgg acgccaggcc cgtcgcggac ggcagcagga agcaggtacg cagcacgttg
    14941 ttccacttcg agctctcctg cacaatcatg gccatgccca gaccaaggac caacaggaag
    15001 accgtgacga tcacggtgta ctcgagcgtg aaccaaatcg agggccagaa caatttgttc
    15061 tcgaacacct tgacgaagtt gtccgggaag ttcacgccgc ggttgccacc cagcaggctc
    15121 caacgagagc aggccatgac gaccagtaga acaatcggca cgaagaacag gacgataatc
    15181 atcagccagt ccggaagtgc gtacagcaga cctcgcctgg tctgcgccgc cttcgccttg
    15241 gacttatgca cgggcacagc cccggcgctc ttggaaacac tcgacatgtt tctctctcct
    15301 ttgggaaatt actgtgaaat ggtaatcacg gaatccgaat gcaatccgat gtcagggctt
    15361 cgaggaagga ggactccgaa gccccgccaa cacaccgcat tcataaggta tgtagtgatg
    15421 aaggaagaga aggaatgaag aagaagatag aagttcgatt atgtggttga gccggctcct
    15481 gccctcacaa gggagggacc ggctcaatct gttcagttgt tattcagtta ttgctgtgat
    15541 ttgatgagtg atggtgagga gcatcaccac cgaatcactg ggcagacagg acgtcggtga
    15601 cggccttgtt gtcggccttg aggtcaccgg aaccccacac ggcgttctgc acgagcagct
    15661 gccacgggga gccagcggcg ttgaaggctt cgttgaaggc cggggacttc ggggtctggc
    15721 cgttgccgtc gataatcacg gagttgatgg tctggatgcg tgggtcagcg tctttgtaag
    15781 cggtcttcag ggtctggatg ttggaggcag tgtcgccttg gtcagcgaag acttccttct
    15841 gagcgtcaga ctgcatcagc cagtagagga agttccaggc ctgagcaacg tgcttggagt
    15901 ccttggagat acccatggca tcgccgccga ggaacgtgga ggtcttgcct tccttggtgc
    15961 ccgggatcgg agcaacgccg acctcgaagc cgccgtcctt ctcagcatcg aacagagcag
    16021 tggtggaggt gttcgggtaa ggcataacgc cgatcttgcc gttcgcgaac ggagcggtcc
    16081 aggtggcgcc ggtctcttcc ttggagcctg cacccaggcc gttggtggtg ttggccagct
    16141 ccttgtaagc gtccaagacg cccttcatgg agtcgttgtc cagggtggcc tcggagccat
    16201 ccttgttcat gacggattcg ccgtcagccc acacggacgg gaagaggtcg aagaccagag
    16261 caccaccgga ctgaccggcg aggtaggagc ctgcgacgcc atccttgttg agggcggcga
    16321 ccttcttggc ctgctcgacg aactggtcga tgctcttcgg accctgctcc gggtccagac
    16381 cggcttcctt gtacaggttc ttgttccaga ccatcacgga aacgtcagtg atgaacggca
    16441 gggtgtactc ggcgccatca acagtaccgg cttcgatgtg gccctgctgc aggtcggcct
    16501 tgttgtccag gccatcgatt tgcttggtga tgtcggtgaa gatgccctcg gaagcccagt
    16561 acggaatacg gaccacgtcg ccggcgagga tgtcaggcag ggagtcggtc tgcgaagcgc
    16621 cgccgacctt gccttccata tcgtcgttcg ggatgatctc gagcttgacc tggttcttgt
    16681 ggctcttgtt gtaagcctcg accacgttct tggcctgacg ctccagaggg gaacgggtcc
    16741 acagggtgat ctcggtacca tcgtcggtgc ccttggccgg gataccttcg gcggtggcag
    16801 tgccaccatt gccggagcca ccacaggcgg ccagcggaac gatcaacgcg gcggcagcga
    16861 tgccagcaag agtacgggtc agtttattga aagacaccat gttcgcttct ttctctatat
    16921 atccgctcaa ctatgagagg cggtggcgga cggtccgcct ccttggtttc gccctgctcg
    16981 ctcctttgct cggaacaggt aacactgcga aagccttttc tcaatataaa cgaacatcgc
    17041 catttttgca aaaacgcttt cgcactttcg catattttcg catatcgaac aggtttgata
    17101 tgcattttgc gcgattgttt gattcaaaca aacacttatt tttagcgctt ccttacggcg
    17161 tgtcgccaaa agccaacata ttcgaacgtg aacacaacac tttctttcat tctcttttca
    17221 atcgcacaaa aatgatcact gcgccacgcc aaaaaataga cttgcgaaag ttttcgcgtc
    17281 atgtctacaa tagaaaacaa tacaaagggg tatcggtaca atcactactt tcgcacgaaa
    17341 gagtgcagca atatactgct gagaaagaaa cgaggaatcg aggcattatg gcccagacat
    17401 tgaccggacg cgcggcacgg ctcgaagacg ttgccgcctt ggcgggagtc tctttggcca
    17461 ctgcatccaa ggcactgcac aacaaacccc gtatcagcga agaaaccaag
//
LOCUS       DAWBMW010000018        16270 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_921_length_16270_cov_3.133642, whole genome shotgun sequence.
ACCESSION   DAWBMW010000018 DAWBMW010000000
VERSION     DAWBMW010000018.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 16270)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 16270)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16270
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_921_length_16270_cov_3.133642"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    7084..7153
                     /estimated_length=70
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9102..9111
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10523..10532
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13891..13921
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3195 a         4698 c         5054 g         3202 t
ORIGIN      
        1 cgattcagac acgattgagg cacgacacgc ctcaaattcc aacgttttca ggctcttgca
       61 ctgcgggctt ccgataacac cgaaaaagtg acttacaatt ccttcacaga atctcaggtc
      121 aatatgcctc atcgccgttc ccctcgttcg cgcagcaggg gcacgagccg actgaggctt
      181 acacgcccgt tcgggtcggt ctggagcttg cctttgtgga tccagccgcg gatggtgctg
      241 gcgggcaggc ggatgccggc cttggcgcat tcgcggctgg gttgggcggg agtgcccgtg
      301 ccctcggttt cggcgagtcg ttcgagcagc gcgtcggcca cgtgccatct gccgattgtt
      361 ttgcttcaat gtttgttcga caggttcggc agtgtttttc cgtagtgtgg gctgatggtt
      421 ttccccggtg tcgtcatcgt ttcccggtga tgtgataggc atcggtgccg cggcctgtat
      481 attcccgagt tgccaatcgg ttttgattcg gccggttgcc gccggccggc gctcgggaag
      541 gaaaggaaca tggcgatacc gatgcccatt gtgcaagata tcaggagact cgaccggcag
      601 ggaatgtcgc gcgcgcagat agcgcgtcgt ctgcatgtgg atcgcgggac ggtcgcgaag
      661 tacgcggata tggaggattg ctcgcccaag ccgaaggcgg atcgcaggta cgggtcgaag
      721 atcgacccgt acgcgcatct ggtggacgag tggctggagg ccgatcgtct gctgcccagg
      781 aagcagcggc acacgatcag gcgcgtgcac gaccgtctgc tggcggagac ggattacgac
      841 ggcgagtatt cgaccacgat gcgttacgtg caccggtggc gcgaggcgaa ccgcggcgtg
      901 ccggatcgcg aggggtacgt gcggctcgag tgggcggcgg gcagcatgca ggtcgatttc
      961 ggcgtggccc gggcccggat cgctggcgag atggcggacg tgcattgcct ggtggtctcg
     1021 ttgccgtatt cgaacatgcg gttgtgcgtg gcgttgccgg gcgagaacgc ggagtgtctg
     1081 tgccatggcc tgatgctcgt gttcgagcat atcgggggtg ttcctcccgt gatcgtgatg
     1141 gacaacgcga ccggtgccgg ccggcgcaac gcgaagggcg aggtcgcgtt gaccggggtg
     1201 ttctccgcgt tcgtggcgca ttaccggctc gaggtccggt tctgcaaccc gtattcgggc
     1261 aacgagaagg gcagcgtgga gaacgcggtc gggtttttgc ggcgcaatct catggtgccg
     1321 cccatgcgcg cggaatcgta tgggcagttg agccgtctcc tgctggagcg gtgcgacggg
     1381 ctcgccaggg actcgtattg cccgaggttg ctggacgtgc ccgtggccga ggtgttcgac
     1441 gaggagaggg ccgcgttgat gccgttgccg tccacggcgt tcgacccggt tcgctgggaa
     1501 agccggacgg ccgacaagta cgggctggtc gacatcgact cgaaccggta cctcgccggc
     1561 cccgattcgg cgcgttccag ggtattggcc gcgatccggt gggacacggt cacgctcgca
     1621 tcgcccgcca ccggcgagct cctcgcggag tatcccagac agtacggacg gtcgcgcaat
     1681 gtggaggatc ccgcgctcgt gcttccccgg ctcgcggtca aaccccgcgc gtggcgggaa
     1741 agctcgatcc gcccggacgt gcccgacgat atacgcgcgt ggcttgattc catggacgaa
     1801 aagacgttga gggagagcct caaagcgatc ggggacgcgt gccgggcggc cgggttcgat
     1861 cccgcgatgc aggcgtgcgg cgagatcctg cgctcgaaca gggacatggg cctgcacgcg
     1921 gactcgctca ccccgatcgc gttgcgcatg cgcgacggcg agtgggaata ccccggcggg
     1981 atcgaggagc ccgacctgag cggctacgac cggttcatca ccggcacgga cgacggaggg
     2041 gaagaacggt gagcgtcaga cccgatccgg tgatcccgga gacccgacgc aggcgcgcgt
     2101 ccacgaccga gaaaagcgaa cgcatcctga agatgagccg cagcctgacc ctgacacgca
     2161 gcgtgctcgc cggcacgctc gccgaggcca cgcccaacca gctcgacttc atcgaacgat
     2221 ggttcacggc cgaactcgac tcgcgcgagc gatccaaacg cctgcgcctg ctcaaacagg
     2281 ccggcttccc cgccgacaag accctcgacg gctatgactg gaccaacctg aagatgcccg
     2341 ccgactgggg gcgcgcgcag ctcgagaacc tcgacttcgt cgccggatgc gaggacctcg
     2401 tgctctacgg gcccgtcggc accggcaaga gccacctggc catcgcgatc ggacggctcg
     2461 cctgcgagcg gggcgtcccg gtgcgcttct tcaccgcgac cggactgctc atgcgtctgc
     2521 gccgcgccca gcaggagaac cggctcgacc gggaactcgc gagcatcggc aaggcccgac
     2581 ttctcatcat cgacgagttc ggctacctgc ccatagacga ggagggcagc aggctcctct
     2641 tccagatcat ttccgattcc tacgagacaa ggagcatcat ctacaccacc aacatcgaat
     2701 tcagcggatg gggacgcgtg ctcggcgaca agaacatggc cgccgccctc atcgaccgca
     2761 ccgtccacca cggacggctc atcagattcg agggccgctc ctaccgaagc gaacacgccc
     2821 tcatgaccaa ataaccaaca caacgcagac aggcagcggc cgataccgca caccctgcgg
     2881 aaaacccgct gcctacactg cggaaaaaca ccgctcacaa agcggacgcc aacttgctaa
     2941 aacacaactc ccggccgcta caattccgcg ttcttcgagc acgcctatct ggcggagaaa
     3001 accggtgcgg cgttggcctt ccccgaagac ctcgaagtgg tggacaacaa ggtctacttc
     3061 ctcgactacg ccggcaaacg ccaccgtgtg ggcgtggtct accgccgcct gtccgacgaa
     3121 ttcctcgacc cgttcgcctt caatcctgat tcggtgatcg gtgtgcccgg tatcctgtcc
     3181 gcctaccgcg cgggcaacgt ggccatcgtc aacgcgccgg gcaacggcgc ggccgatgac
     3241 aaggccatct actacttcgt gccgcagatg atcaagtact atctcggcga ggaaccgatt
     3301 ctcaacaacg cgcccacgta tatgccgctg ttcgaggccg accgtaagga agtcctgaat
     3361 cgtatgggcg agctggtcat caaggatgtg gccgaagccg gcggttacgg cgtggtgttt
     3421 ggttcgtcgc tggatgccgc cgcacgtgag gagctcgcca accggatcaa ggaggagccg
     3481 cgtcgattca ttgcccaaga ggtgattcag ttccgtgata tcgatgtggt ggacccgaag
     3541 accggtgaga tgtcgcctcg caagtgcgat ttgcgtgcct tcgtggtcac cggcaagaat
     3601 acgcatgtgt ggtattcggg cttgactcga tactcgtcgg tgcccggcca gatgattgtc
     3661 aactcgtcgc agggtggcgg attcaaggat acgtgggtgt tggcacccga atccggcgtg
     3721 gagcatgaat acggtgctga agtgcaggtg gcgaacctgc tgagccagtc ccgccgccac
     3781 tcgttgtctc tggtcaccgc ttccaaggca gacaacctgt attggctggg tcggtacacc
     3841 gagcgcgcct tcaccacgct gaaccagttc ttcccgttct acgatcgtgt gatggacacc
     3901 gatgtggacg ccttccgtcc gttcgctcac gcactcgacc tgcccgaaga tttcgaggac
     3961 ttcgacgggt tcgtgaacag cttcctgtat gacgggtcca atcctgattc ggtgcggtcg
     4021 gccgtaacct ccgcattcaa caacgccgtg attctgcgcc cggaattgag ctcgcgtctg
     4081 ctgcagtacg tggagttggc gatgaccaat atcaccgatg ccgccaaata cgcggccgat
     4141 gccgaggaca tctacaagca gcgcgatatt accgatgaca tgctcgcctt ctggggcggc
     4201 atcgagaatt cgccggtcga ccccacgctg aaggcgttca tcttcatcgg caagtatctg
     4261 gaacgcatcg acctgtacac gcgcttcggt ttgacgatgg aggagatgga agcgccgctc
     4321 aagaagctcg ccgcctattc gatgaccttg gacggcatgc cgctgcccag ttgcttcgcc
     4381 gatgggctga gctggctggt cggtcagctg ccgtctcgcg gctaccagga gatcgccgac
     4441 ctgctcaaga agtatctcga cgactactcc ggccgtgtca gcgcgctggc catcaaggac
     4501 atggggtccc tgagctccat gaacatggac gccaagcgtc cctgatcccg acctgacacc
     4561 ctgacgggtt caaatgctat ggtggtatgg cgaaaaaacc gtgcgcccga gtgccggcct
     4621 gtgcatatca gcagattgcc ggaatatatg cgtgggctgt gagcgcacgg accatacctc
     4681 agccatggct gtagtgttgt tatcccgaga atatgccggt tgccggctcg tgtgtaagtg
     4741 caggagacgt tgtgaaaaaa ctcgtgttcg actatgagat gaggctgtcc ttcagcgcac
     4801 cggtcaccga tcacaggttc cagcttcgct gtattccctc cactggccct cgccaacagg
     4861 tgattgacgt ggaagtgaag attcagcccg aaaccgaact cgaaaccacc atcgactcct
     4921 ttgactccgt ggtgatgacc ggattcatcc ctgagccgca cgacatattc agctactcgg
     4981 tgaccggcat cgccttcgtg gacaatgcgc atatccataa ggaagcctat aaaccgctgt
     5041 accgattcaa ttcagcgctc accattcccg gcccgaacgt ggaggcgatg attgccgtat
     5101 gccgcgaacg cttggcggcg ctgccttccg acgccactcc ggtgcagcag gccgccgaag
     5161 tgatggatga ggtctacaag gcgtttgtgt acacgcccgg ttccaccacg attcgcacca
     5221 ccgccgaaca ggccttggcc cagcgcaagg gcgtatgcca ggattatgcg cacgtgatgc
     5281 tgtccgtgtg ccggcatgtg ggattgaccg cgcgttacat tgccggtttg cttgggggag
     5341 agggcgcgac ccacgcttgg gtcgaggtgt atcaggatgg ccgatgggtc ggactggatc
     5401 ccacgcataa ccgattggtg gacgacagct acatcaccat cgcccacggc cgcgattacc
     5461 gcgactgcat gctcgacatc ggcatcttct ccggctacaa cgtccagcag tcccaatggg
     5521 tcaacgcctc cgtccacgaa caggtggcat aaacaaaatt ccaagccccc tctgatgagc
     5581 tgagccgtga agaaaacgcc ggaggcgttt ttaggcgaag gcgaacgaca gtgagcaaat
     5641 aaaatggcag ctggaaagct gtccttaatt gcgttcgagc tgtcagccgt aaggctgacc
     5701 gagggagaga ccactcaatg cagagtggcc cccgtcgccg cttctaccac cgccaatatt
     5761 cggattttcg tcgttgcgac attcggattt tggtagttga agaattcgga ttttcgcagt
     5821 tgaccaattc ggactgttaa tcggctcatc gtctgaccca ttgcaatctc acacaaaatc
     5881 gcattatccc acgccgaatc gagctcagcg aaactccgat ttgccaggtc ggcagaatgt
     5941 ggcataatga acaagttgcc attttggcag catggataag tgtccgagcg gtctaaggag
     6001 cacgcctcga aagcgtgtga ggtgaaagcc tccgcgagtt cgaatctcgc cttatccgcc
     6061 tttagggttc cgggtttgtc ccggaaccct ttttgttttt gatacgaacg tccgtattcg
     6121 ctcctgtgct gtatcgtata cggcgtgtcg ggtggaaaat agcgaaaagg ataacgttgt
     6181 actcagcggg acttgcgtac taagtacaac catcgtatta tcgatatcaa cagaagtaca
     6241 aggttgtatt aaatatccag aacgcacatt caccgctgat tggcgatgcc gcaaaacagg
     6301 tgaaccaaga agatgggccg attgcttttg gtatttagca caacgttgtc ttgcggtatt
     6361 gaagacaacg attaaaggag agcacatgtc cgagcattcg cacgacaata aaccggggga
     6421 gctggaggaa tctcgactcg tcgtcgataa tgactttgaa gtagctccgg tcaacgacag
     6481 actgttcggg tcgttcgtgg agcatctggg tcgctgcgta tacgacggca tctatgagcc
     6541 gggccacccc gaagccgatg aagacggttt ccgcaaggac gtcatcgaac tggtcaagga
     6601 actcggcgct accacgattc gctatcctgg cggcaatttc gtttccggct atcgctggga
     6661 ggatggcgtt ggtcctcgtg atgagcggcc gcgtcgcctg gacttggcat ggcattccac
     6721 cgagaccaat cagtttggtc tgcacgaaat ggcgaaatgg ctggaaaaaa ccggcggcaa
     6781 cgagctgatg gaagccgtaa atctgggtac ccgtggtctt gaagaggccc tcgacctgtt
     6841 ggaatacgcg aatattcccg gcggcaccaa gctctccgag gagcgtcgcg ccaatggtgc
     6901 cgatcagccg ttcggcatca agatgtggtg cttgggcaat gagatggatg gcccgtggca
     6961 gaccggtcac aagtccgccg aggattacgg caccttggcg gcctcggtgg cggcaggtat
     7021 gcgcgccatc gatccgaacg ttgaactggt ggtctgtggt tcatccagcc acgtgatgga
     7081 cacnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7141 nnnnnnnnnn nnngggaaga aaccgtactt gagaagacgt ttgacaacgt caacttcgtc
     7201 tcctgccatg cgtactacca tccggaactt cagcctgatg gcacccgtga catgaagagc
     7261 ttccttgcct cgggcgtcga tatggacggt ttcatcaacg atgtcgccgc cgccatcgat
     7321 gcaaccaagg cccggctgaa gagcaagcac gacgtgttca tctcgttcga tgaatggaat
     7381 gtctggtatc tcaacgaaga gccgagcaag aatccggaag gcatcggcaa ttggccggtt
     7441 gccccgcgcc tgctcgagga tgtgtacagc gccgccgacg ccgtggtgtt cggcgatctg
     7501 atgatcacgc tgctcaagaa cgccgaccgc gtgcgcgccg ccagcctggc gcagctggtc
     7561 aatgtcatcg ccccgatcat gacggagccg ggcggcccgg cctggcgcca gaccacgttc
     7621 cacccgttct ccgtgaccgc gcgcctggcc aagggcggca ccgtgctgga gccgaagctg
     7681 tccgccggca ccgtggatac gccgcgctat ggcgaggtcg acggcgtgaa cgccgtggcc
     7741 gtgcgctgcg ccgacggctc gctcgccgtg tttgcggtga accgttcgct cgaggcgacc
     7801 gccgagttcg agatcaggct gcaggacggc gcgcagccgg catccgtcga ggcgcagacg
     7861 ctgcacgacg acgatctgtt cgccgcgaat acgctctacg accagaaccg tgtgaccccg
     7921 cacgccaacg ggtcggccat gtacgatgcc gagtccggcg tggtccgcgt ctccctgccg
     7981 ccggtgagtt ggaccgccct gcacatcaag tgacgcgatg ggccgcgcgg tccgttccgg
     8041 agaaggacgg atcgcacggc ccttgcttcc cgggccgggc ggaagggacc ggaccggccg
     8101 gggaaaacgc gttatgctgg aatcagacgg gcgttccgcc tgaaccgtgc ggaacatgcc
     8161 cgatggtgcg acgagagggg tgctgtggcg aaacgggtga ccatcaccga cgtggcgcgc
     8221 gagacgggcg tctccatcaa aacggtgtcg aatgtgctga acaacaccgg cagtatgcgc
     8281 ccggagacgc gccagcgtgt ccaggatgcc atcgaacgcc tcggataccg cgttaacgtg
     8341 tccgcccgcg ccatgaaaac cggcggcacg aagctcatcg gactggccat caccgatttc
     8401 tgccagccgt tcatgcccta tctcgccgac agcatcgtgg cggcggcacg ttcccgcggg
     8461 tacgcggtga tcaccgatac ctacaacagc gccggcggct tggcgcaggc gatcccggag
     8521 acgaagaagc tggccgcgga cggctggatt ttcttcatgg gctcgccgat cacggatcgc
     8581 acgatactgc accagccgta tccgttggtg attgtgggcg attacgatac gcagggactg
     8641 gccgattggg tcacgatgcc gaacgtggag gccgtacgca ccgcggtggg ggcgctgctg
     8701 gattcgggat gccgtaggat cgcgttgatc ggcggcaccg cggagaacgc atgcccggat
     8761 gccgaccgcg agtcgcggcc cgaaaccacc agcacgatgc gtacccgcgg ctatgtgttg
     8821 gcccatgagg agcgtggcct gcaggtcgat tggcggctgg tccgtaacgc ggattgggtg
     8881 cagtccggcg gccgcgatgc ggtggcgggg ctgttggacg atccggatgc gccgcgtccg
     8941 gatgcggtca tgtgcctgaa cgacgcgctc gccatcgggg tgattcatga gctgcagcgg
     9001 cgcggcgtgc gcgtgccgca ggatatgcag gtcattgggt tcgacaacgt gccggatgcc
     9061 gagtactgca tgcccggatt gacgacgatc gacccgcatg tnnnnnnnnn nacgcgcgac
     9121 atgccgtgga tatgctgatc gcgcgcatcg aggggtcggc ggatccgttc cgcacgtatg
     9181 tgacctccta cgatctggtg cgtcgtgcct cgacgtgttg acggcccggc gttcgcctgg
     9241 acatgggtct gtgacgggct gctgtcgcct ctcccgtggc gtattccggg agaggcgaca
     9301 tgcgggctag gatcggagca gcacctccgt cagcgcttga cggacggcgc tcggcatatg
     9361 cgaatcggag caatcttcca tgtccggccc gctgacgtgc tcgagtgaat ccatcgtggc
     9421 caatgcgtcg atgccgttct ccgttatggc ggcataagcc tgttccttgg tcagatatcg
     9481 catttcggca tgccgaagga tgtcgaagac gtcttccatg cggggatcgg gggccatcgt
     9541 cgtgtcgccg atggtgacac gtatctcgct gcgggtggat acgtcggcga ccgttacctg
     9601 caatgtgagg gtttcggcat cataccggag atccgaatcg acggatgcgc cgtcggcctg
     9661 tacggatatg tcgctgtcgg taatgcctcg gaacaggaaa tcccatgttc tccgggccgg
     9721 cagagcgtga acgtctccct gggctggcga taccgtgagt gcggatgtgg cgccgtcctt
     9781 gcgccatcga taggtgatcg cggtggtcgc cggggtgatc tgccgagagt aatgtcccga
     9841 atcctccatc agcgtgaagt cgccgtccgc tccggggaag acgatgatct ccagatgctg
     9901 cgggttgtcg acggagttga tggaatcccc ctcggacagg ggctgcatcg gcacgatgcc
     9961 gccggccttc gcgaacacgg gaattccgtc gagcggacgc cagacggtca tgcgccgtcc
    10021 gttgggcgaa gacgcgctat accggcgccc ggtgaagaag tcgaaccagt cgccctgcgg
    10081 cagccatacg tcggccttgc cccgtcggga ggatttatcc atcggttccg tgatgggcgc
    10141 ggccagcagt tcggtgccga acatgtattc gttgggaacg tggtatgcgg cgtcgatgtc
    10201 gggcgatccc caatacatgg gctccaccag gggaaggcct gtccgggatg cgcgccagtt
    10261 catggtgtgg aggtacggaa tcatgcgatg gcgcaggcgg agcgcatcgt ccatgatcgc
    10321 acggatctcc gggcggaagt tccatggttc cttgccggag aacggcgagc agctggaatg
    10381 caggcggttg atcgggctga acacgcccag ctggtaccag cgggcttcca gctcctcgtc
    10441 gcggtagccg aacatgtggc cgccgatgtc atggctccac cagccgtaac cgatgttcga
    10501 cgcggtggcc gtgaaatacg gcnnnnnnnn nnagaagccg accggatacc gctgcgagcc
    10561 ggggccggcg aaccgggaga ggatcagcgg ccagcggccg tcgcgcgcgg aatcgcagta
    10621 gtgcatgtgg ttgagcaccc acagcgggtc caggccgggc tggcgggtca cgccgccctg
    10681 ctgccagtcg atccaccaga agtcgacgcc gtcggcctcc aggtcgtggt gcatgtcgaa
    10741 gtaggccctg acgaagcgcg ggtcggtcag gtcgaactcg accggctcgc cgctgtccgg
    10801 ggaaatcccc atcgttttct ccgctgaagg atagcagtcc tcgaacgcgc ggatgcacgt
    10861 tcagggagac gtggcagccg cgctcgtgca gatcccccag aaaacgttgc ggatcgggga
    10921 tcagcttgcg gttccaggtg tagccggtcc agccggagcc gtacttgggg tcgacgtcgt
    10981 ccaccagatg ccagtccatg tcgatcacgg aggtggtgaa cgggatgccc tcggacttga
    11041 accgatccat caatgctaca tactcttcgc ttgtgtagcg gtggaaacga ctccaccagt
    11101 tgcccaaggc gtatcggggg agtagcggcg accttcccga aagacggcag aaatcggcca
    11161 ccgcatcggc ataacggtgg ccgtgcccga agaagtacag gtcaatcccg gcgttcttcc
    11221 ggggcttgat gtcaccattg tcggcgaata ggcaggtgtt cgaatcgtcg agaaccgacc
    11281 agccgtcgcg ggagaccacg ccatcgttca ggggaatggc gccgtcggcc caatcgagcg
    11341 tgcgggccgt gcctttcatg ttgccctcct gcgcatcgcc gaaatgccag gtattggttt
    11401 tggtgtcttt cacgcctttg accacgatgc tgagtccctc cttgctgaag ggccgcatgt
    11461 cgtaggtcag acgtagcgcg gtcgtttcca cggtcagcag accgtcggcg atcgaggtgg
    11521 aatattccgg gggtgtgccg aaatcgcggt tgacggccat gagcgtcgca tcatcgttga
    11581 actcgccggt gtctgaccat tccagtcgta gcagcgaatc ggagaggact ccgattctcc
    11641 agcagttccc tgaaatgacg cctttgctgt tcgtgaaagg ttgggtattc atacggatgc
    11701 actcctgtgt tttcagtgat tctgtgctaa taacatagca tttttcaaag agagtttgca
    11761 accgtgtgca aaatatgcta ttttcaagac aagaagattt cggtcttcgt tgcaatatga
    11821 cgctgcgcat ggattcaaga gtgaatcttc tgcagatatc gatctcggag aggagatgca
    11881 gacaatgacc actgtcggaa aagtggtgcg cagagggtgg gcgctcttcg cactacctac
    11941 agtgttgtgt ttcgcggtgt tcttcgtcgt gccgcttgcc atgggtatct acctgagctt
    12001 tacctcgttc aggacggtga ccgatgccaa gtgggtgggg ctgaagaatt atgtggatgc
    12061 gctttcggat cccgaattcg gagccgcgct gtggcggtcg gtgctgttca ccatcgtgac
    12121 gaccatcgtc gtcaatgtcg tcgcgttcct gctggcctat atgttcacca aggcgatgcg
    12181 cggggcgaat ctgttccgct cggtgttctt catgccgaac ctcatcggcg gcatcgtgct
    12241 gggctatatc tggcagctgc tgttgaacgg cgtcctcgcg ctgtgggacc gttccctgac
    12301 cacgtcggcg gtctatggct tctggggcat ggtcattctg ctgtgctggc agcagattgg
    12361 ctatatgatg gtcatctaca ttgcaggctt ccaaagcctg ccgactgatc tgatcgaggc
    12421 cgcgcaggtc gatggcgcat cgaacggtca gattatgcgc aggatcatca tcccgctgat
    12481 gatgccgtcg gtgacggtgt gcagcttcct gacggtggcc aacggattca agatgttcga
    12541 ccagaacctg gccctgacca acggcgcgcc gtcaaacgca tccgaaatga tcgcgttcaa
    12601 catctaccgc accatgtacg gccgcaccgg attcgagggc gtcgcgcagg cgaaggccgt
    12661 catcttcttc atcatcgtgg ctgtggtcgc attcatccag aaccgcatta cgaccagcaa
    12721 ggaggtcgag gcatgaccga caaactcagg cacgggccgc tgtggacgac cattttcgcc
    12781 atcatctcgc tgatatggat ctatccgatc gcgctggtct tcatcaactc cttcaaaaag
    12841 cgcgtctaca tctccaagga cgtgttcagc ctgccgaccg gcgacacatt cgtcgggttg
    12901 gagaattacg agcgcggcat agaaaagacg aatatccttc cgagcttcgg atggacgatc
    12961 atacttacgc ttgggtccgt ggcgctgatc ctgctgtgct gctccatgtg cgcatggtgg
    13021 atcgtcaggg tcaacaactg ggtcgccaag cttatgtatc tcgcgtttat cgcaagcatg
    13081 gtggtgccct tccagatggt catgttcccc ctcagcaagc tcaccgacac attggacctc
    13141 aacacgccgt ggggcatgtg gctggtgtat ctgggattcg gcgcgggatt gccggtgttc
    13201 atcttcaccg gcgtgattaa gtcgattccc tcggagatcg aggaggcggc catgatcgat
    13261 ggcgccggct ccatccgcac gttcttcacc atcgtcctgc cgatgatgcg tccggctctt
    13321 atctccgtgg cgatcctgga gacgatgtgg gtgtggaacg acttcctgat ggcctatctg
    13381 tgcctggatc ttcaacggtt caagacgctg tccatcgcca tccagtacct gaagggcgga
    13441 tacggatccg tggacatggg cgcgatgatg ggcgctctgg tactggcgct gctgcccatc
    13501 ctggtgttct acctgttcgg acagaagtac atcatcaagg gcgtcaccgc tggggcggtc
    13561 aagggctgag ccccaatcat ttcaagaacc aatcggttca tcaaggaaag gaaagaacca
    13621 tgaaaaccaa gaaaatgatg gcgatcgtcg ccgcggccgt catggcgatg ggcgcgatgg
    13681 cggggtgcgg atcctcgtcg gcggccgatc cggacaaagg caaggtctac ttcgtgaaca
    13741 acaagaagga gatcgtcgac cagttggagg atctcgccga tcagtacacc aaggagacgg
    13801 gcgtgcaggt caaggtgctc acggccgccg acggctacga caatacgctg gcctccgagt
    13861 tggccaagac cgaagccccg acgatgtgga nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13921 nccccaattt catcaagtgg aaggactaca tggagccggt ccaggactcc gccgccttca
    13981 agctgctgac cgacgagggc aaggccaact cgtatcaagc cgacggcaac cactacacgt
    14041 tgccgtacgc cgcggaatgg ttcggcatca tctataacaa gaagatcgtc aacgactacg
    14101 cctcgaagga ctatgcggtc atcgactccg ccgacgatat caaggactgg gatacgttca
    14161 aggccgtggt ggaggacatg caggcccata aggacgatct cggcatcgaa ggcgccatgg
    14221 cgacccccgg actcgaatct tcggacctgt accggttcgc atcccatatg acgcgtattc
    14281 cgctgttcta cgaatataag gacaacaaca ccacgttcat gcctgagatc aagggcacgt
    14341 acgtggacaa ctacaaggcc ctgttcgact tggagatgaa caactgtccg acctcgcgca
    14401 cgctgttgac ctccaagacg tatgaggacg tgaccgcgga gttctcgctg ggagaggtgg
    14461 ccttctaccc gaacggcgta tgggcctaca cgcaggtcaa ggacaacgag gtcgccgata
    14521 aggatctggg catgctgccg tatttcatgg gtatccccgg cgaggagaag tacggccccg
    14581 ctggcatcta cgacgcgtcc tgggccgtga acaagaacgc cagcgagaag gacaagcagg
    14641 cgaccttcga cttcatcgag tggctgttca gcagcgacga aggcaagaag gtcatgtctc
    14701 aggatatggg cttcgccgtg ccgagcaccg cgtacggaga taaggatcag cccgacaacc
    14761 cgctgaccgc cgccgcccgt aagtacgaga cgaatggcgt tccgatggtg aggagcttct
    14821 ccacgcttga cgcctggggt gcagacgtgc aggacgctct tgtggagtac gtcaaccaaa
    14881 ccgggaagtg ggacgccgtc gccgacgcat acaccaatgg ctgggccaag gagtggaaga
    14941 ccttcgagga caccaacggt gtgctgccgg aggccaccac gttgaatggc gacgagcagt
    15001 aaatcgttcc tcctccccat ccccgaagcg gggccgtatc ctctctacgg ccccgcttcc
    15061 cctattcggc gcctattcgt cgacgtcccg cggagtcgcg gtggtggatc ggatgatcag
    15121 ctcggccgga taggttcggt acggttcgga aatcgtctta tgattgataa gatcgagcag
    15181 tttttccgcg acgtcgtgcc ccatcaatcg cggctgctgg cgtatggtgg tcagtcccaa
    15241 ttcctgcgag taggttccgt catcgaaacc ggtgatcgag atgtcgccgg gaatcttcaa
    15301 cccgaattcc cgtaaacggc acatcaatgg tatggcgatg ccgtcctctt ggcagcagat
    15361 cgcggtgggc atgggctgca tattgatcaa tgcgttgaag gtcgactgca acgaatcgtc
    15421 tccgtccgga ataaccagaa cgtcgtcttt gatatgggcc tgcgcgcagg cctccttgaa
    15481 tcccctgatg cgctgagccg cacttaatgt cagcaacgat tgcgggtccc tgcacacgta
    15541 tgtgatgcgg cgatgcctca gggacaccag atgccggacg ataagccgca gtccatgcgc
    15601 atcgtcgatg gccgcccata ccgacatgtt ttcgttggag acggtgttga tgcccgcaat
    15661 gggcacattc atcgttttca ggcgcgcgac ctctctggaa tccacgttga acgaggggac
    15721 tatcactgcg tccgcattgc gtcgcgccgg cagaacgtca aagaaatccg ccctgctttt
    15781 tacattgttg atggggtaga tggagatatc atagccggcg ggatgaaaga ccatgtcgag
    15841 cccttcgaag atgttgcctc cgaaccatgt cgtgaccggg gtgctcagca gtagcgcgat
    15901 acggaagctc tgcccggact tgagcgccat cgatgatctg gaaacggaga aattgagctc
    15961 ggaggcggct tgcaacactc tgtttcgagt tttttcggag acaagctccg gccgtgtgaa
    16021 ggcgcgcgaa accgtggacg cggagacatt cgcacgaatc gcgacgtcgt gaacagaagg
    16081 tgtggaggtc ataatgctca attatagaag gttgggggat gcggacggaa agtcggacag
    16141 gctaagctgc atagaacacc gcatctaatc ggttcgacac gcgcatttca ctgcgcgact
    16201 ggacattgac gtccctttcg aggtaatatc gaaccggttc gatacatacg tgagtatgca
    16261 aatacgtaaa
//
LOCUS       DAWBMW010000019        15561 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_963_length_15561_cov_2.391719, whole genome shotgun sequence.
ACCESSION   DAWBMW010000019 DAWBMW010000000
VERSION     DAWBMW010000019.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 15561)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 15561)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15561
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_963_length_15561_cov_2.391719"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    12449..12458
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2973 a         4786 c         4714 g         3078 t
ORIGIN      
        1 cctcgccttc gatgacggtg aaggtcatgg acttgcgttc gctggcctgc ggggcgatgg
       61 cgtgcaggtc gccgcgctgc tcgtgccagc ctcggccgtg gacgctggag ttcgagggtt
      121 cgaggccgac cacgtagtcg cccgcacccg tcgacttcca ttccatgaag tatgggaaga
      181 ggctggcgtc gaagtcgatg acgactccga gtccgagctt gcggttgacc acggcggcga
      241 agctgcggcc gttctcgtcg gcggcgagct cgtgcaggta cacgctttcg ggactgatcg
      301 tcgcggaagg attcgttggt cacctcatcc tcgaccgtga tcgacagccg gccgtacacg
      361 ctggtgattg tgcgacgcag tacgaggttc tcgccgcggt attcgaactg gcagacgtcg
      421 agggcgcggt cggccatgga cacgaaacgc aacggaccgt tcttcacctc gatggccttg
      481 aggccgtggg ctcggccttc ctagtattcg atcgggcgca cgtaggcggc ctgctgaagg
      541 ctgcccatat gggccagcat gtcggctcgc gtgctgtgtt ctgtggacgt catcgtcgtc
      601 accgatcctt gatatttatg cctcacgcct gcgcagcggc ggcatccgcg gcgtcgttct
      661 tcagcgcgcg cagcagggcc ttgttgcggg attcgatgat gcgcttgccg tcctcgccct
      721 gccaggagaa gaagccctgg ccggttttgg cgccgagatt gccttccctg accttctcgg
      781 cgagcagcgg gctcgggccg gtgccgttgt ccatatcgtc gaacaggtaa gtgctgatgt
      841 tatagaacac atcgaggccg ccgaggtcga tgctggcgag ggggccgacc agattccagc
      901 gacggccgag actgtacttc atcacgtcgt ccacggtggc cgcgtccgcg atgccctgct
      961 ggacgatgta gaacgcctcg cgcagcacgg ccagttgcag gcggttgccc acaaagccca
     1021 gcgattcctt cttgatcttc gccggcttct tgccgatctt ggccatgagg tcgaaggtga
     1081 tgtccaccac cttcggatcg gtcttctcac ccggcaccac ttcgaccagc ggcatcaact
     1141 gcgcggggtt ccagaagtgc gccaccacga agcgttcggg cttcttcagc accgactgca
     1201 gcgcggtggg gctcaggccg gaggtgttcg tggacagaat cgcgtccttg ggggcggcgt
     1261 gctccacttc ggcccagacg gacttcttga tgtcaaggtt ctcggccacg gattcgatga
     1321 cgaagtccac gtccgccacg ccgctcgcat agtcggcgta accggtgatg cgggccagca
     1381 ccgcgtccaa ggtgtcgcca tccttgagta gtccggcttc ggcaaagtcc tcggcatccg
     1441 aacgaatcgc cttcatcgcc ttctccagcg aggcctcgcc gcggccgacc agatgcaccg
     1501 gatatccgga caacgcgaac tgcaacgtga tcgcgtggcc catggttccc gtgccgacgt
     1561 tcgcaatcgt cttgatatcg tccactgtct caatcacgga gaccccttct ttccggcggt
     1621 tcgtctggcg ttggtgtacg catgaacagc accgtgccgt tatgcgtttg cttgcattct
     1681 atgccgattt cgctacgtga acatttactg aacatttcgc ggattcgact agtacaaatc
     1741 cccatatcag cacaaaagcg tggtgccgca acaccgaagt gttccagcac cacgctgaaa
     1801 tgttcatgtc gttacgctgc tatcgcgcac ccacagcgga atcgatagga aacctcaccg
     1861 gagtaggtga acacgggggc gaccagcatg gacggaccga acatatactg acggtccagc
     1921 gtgcggcatg ccgggtcatc ggggaactcc acgaacatgg aacgcatgac cggagtaccg
     1981 ttcacatgag gctgcaggcc aagctggtag acatacggca tgagttcaag cttgagcttg
     2041 gtgaactcgc gcaccacatc cacggcggtc tggccgggca ccaatgcgac gccgttcttc
     2101 tcgtcctcct cgtcgaacag ccacggcaca cggtacacgg tggaaccgtg cagtcgcgag
     2161 tgggagccga gcatgccgaa cgccacccaa cgcttgtaga cggccggatc cgggaacgca
     2221 ccttcgaagc caccgatgtc gtgactccag aagccgaagc cggaggaggt cagcgacagg
     2281 ccggcacgca aggactgggc catgccattg aaggtggact cgcagtcacc gccccaatgc
     2341 acgggctgct gctggccgcc gacggtggcg gagcgcgcat acaggcaggc gttgcccttg
     2401 ccgtaggttt cttcgatggc ttcgaacacg gcctggttgt acagctgggt gtaccagttg
     2461 tgcatggaga gcttcggaga gccgtcatac cagacgacgt cgcgcgggat acgctcgccg
     2521 aaatcggtct tgatggcgtc cacgccctga ttcagcaacg ccttgacctt gtccttgaac
     2581 cattcacggg cggccgggtt ggtgaagtcc accaagccca tgccggcctg ccagaagtcg
     2641 gtctgccaga cttcgccatt gggcttgcgc accagatagc ccttgtctcg gccctccttg
     2701 aacatttcgc cgcgctggcc gatatacggg ttgatccaag cgcagatgtg caggcccttg
     2761 tcctcgtgca ggcgcttcag cgtggattcg atgtcgccga agaaacgctt gtcccactcg
     2821 aaatcgcacc aatggaactc gcgcatccag tagcagtcgt agtggaaggc cgccagcgga
     2881 atatcacgtt cggccatacc gtcgatgaag gagttgatgg tggcctcggt gttctcggaa
     2941 ccgatctcaa aggagacatg gccgcggttg ttgaccagta cgccataccc attggaggtc
     3001 atgtagaacg ggatgtcctt gtagccctgc tcggaagcag tgccgccatc ctcattccag
     3061 atgtcgacgc tctgaccatt cttcacgtaa gcgccgaagc gttcgcccaa gccgtatacg
     3121 tcttcgccaa ccgacagatg cagctggatg gcaatgaaca cgtcggattc atcacgggcg
     3181 gaaccgtcca tggtcacacc gaattcgctg accggctggg cggtgacgtt cgattcggca
     3241 ccgagcttga atcggcccag cgacttgcct gcggactcag tgagcacctt gccgtcctcg
     3301 ccgatgaagg tcaggttcca gggggcgccc ttgacgacct tcacggtaag tccgccagtg
     3361 gtcagcgtga catccgcgcc attgacgccg acttcgccgt cgccattgcc gttcgcctga
     3421 acggtcacgt aatcacggtt gccgggttcg tcggcattga gcgggaatcc ggggtattcg
     3481 gtggcgcctt gccagtgttc ggcacacaca cgaatcacgc cttccgcagg tgtggtgatg
     3541 tccacattga atgtgggcag gttcagggta tcgtatcggc cgcgcaccac ggaggtcggg
     3601 gcgaggacgt tgaggctttc ggtggtggca tccgccgcca gttcgtaggc ttcacgagcg
     3661 tagagggcgt cgacgccgtc acgaatcatc cagtagccgt tggtgaactt cattgttgct
     3721 gctccttggt ggagaggtgg tttcgaccgc cagggaacaa ggcagccgaa accaaaatca
     3781 atatgtgctg cttgttgatt gaattgcctg ttgtgcgaat gaccttactt caccgcgccg
     3841 gtggtgatgc cgtaccagtc gccggagccc atcatgcctt gctcatcgta cttgccggtg
     3901 tcggccaagg aaccggtgat aatcttgccc cagccatatt cggacacgta atcgttgagg
     3961 ttggtgagca ggccttggct ggcgagcagg ccggacgtgg cgttgccctt gttgtattcc
     4021 gtaacgtccg gagttgagga cctgggaggc gttctggcga atctgttcga aggccttctt
     4081 ctcgaattcg accttgacgc cggtctcaca cattttcatg ctttatgcaa aaaatgtgag
     4141 gcatacgcac atttcccatc aaacgcacag gtgcaagaat ggcagcaatt cgccattttt
     4201 gaaatcacca tgtgcgtttg acggcgcgta agcctcacat tgttctcagc aggaccgaat
     4261 ttctgcgagc ccccccccta tagcgctccc ctacgcaata tgtccgcaaa cgaccacacc
     4321 acatgggcga tacgcgccaa ttcggcgtaa cggccgatgg acagccactg cacacggtac
     4381 cgttcgaacc acaactccaa atcggcggca agacgggcgc ggcgcgaggc atccagcgca
     4441 cggccggtca catccttggt gtcacaggcg tcctcgccgc cagccaatac cagcagttcc
     4501 tcacccagtc tattgaacaa ccgctgccca tccagcatca cccacagcag ctccgggttc
     4561 aatcctcctc gtgccgagcc atcgagcacc gcgacaaccg cactgccgca atcaatcaac
     4621 gcccggtcgc aatcccggcg tcgttcgaga cgggcatggt tccgcaggag caatcggcgg
     4681 cgcgcgtcgg cgcatcccag cgcacggtca aacatgagct ctcccccgca cgaccgttcc
     4741 acatacgatg ccacatccgt attcagcatg ccggtgccgg aaccgtattc cagttccagc
     4801 acttgcacgg cgaggtccca ggggaacgac actccatcgg agtcgatgcg agcgagtgaa
     4861 tccataagcc aaccggattc gtcgccatac gccagattag aaatccgatg gttcatttcg
     4921 catgcgtcaa ttgcaaccgg gttccacgcg ttttgcgcac cgtagcacag tcccggcacg
     4981 ctcaaccgcg gatcattgac atgcccatag tcaccccaat cggtgaccac catgccttcc
     5041 gcaccatatt tgagcccagc caccgccatg cggtacgtgt tcagccaggc accgtcatag
     5101 tcggggaaga accggctcca cgcacgcacg gcagggcaca cgaattggcg gcggccttgt
     5161 gatgcgatgg tctggatctt gctttcgtcg atatccggct catacatcca gttgagcatc
     5221 gtgatgtcgc cgggcagcaa gtccatggtg tgcggattct cgagcgcgat atccgcccac
     5281 agcatcggtt gtagaccacg atgggcaagc gtgctgcata ggtctttgac gaaatcggca
     5341 tacagctcat cgcgactggc gcccggcgag tcctgtacgg accggccacg gcccaagtcg
     5401 aaagtctcat cgccaccaat attgaaggac cgcgagcgga acagcggcgc gtattcctcg
     5461 attaggcgcg aggcgaaatc gtgcgacttg gggttggctg cgttcagtgt gtggtgttcc
     5521 attcgttcga tgaaactgaa cggacggtcg gcgtcttcgg ggaactctcc cagtccacga
     5581 tgttcacggg tgcgcaggtt catatacatg tgcccgaaag tggccaagga cggcaccagt
     5641 tcgatgccgt ggtgtgcgca gtattcgtcc aggaacatga tgtcgtcggc cgtgagcggg
     5701 tcagtgccgc gccacgcttc gctgagctcg ccgaaagcga aggcatgttc cacgtacagc
     5761 tggaattggt tgtacttata caacgccagc tggtcgatga accaggtgag gaacgccatc
     5821 gtcggcacac gcccgcgcgt cacgtcaaga ctgtaggcgc gcaccgcgaa cgcaggcttg
     5881 tcttggatat gcaggcaggg caaggtccgc gaggtttgcc tgatcatctg ccgcagtgtc
     5941 tgcatgccgt accgcaatcc gctttcgctg ccaccacgca cggcgatggt atctgaggcg
     6001 aacacatcca gcgtgtattc gtcatgcgcc aatcgcgcat ccacacacag caatatgacg
     6061 gtgccgttca tcgctgccgg cgcacccgcg cccgcacccg cacccacgcc cgcatctcgc
     6121 gctttttcgg cggcattggc accggcgtcg gcttcatctg tggctgtgag tactcggcgc
     6181 gtacggacgg cgacgccgca gtaacgctcg agttcgctgg acagctgctc gattggcagc
     6241 gtttcccagg tctcatcggc atcttcggtg acggcatatt cgggaattcg tttctcgatc
     6301 gtcaccaagg acccgatcat agccgtgcca tgcgtgtatt ccaatgtcac cggagcggga
     6361 atcagggtga gacacggcgt tgcggcatca gccttatatt cgaatgtggg cacagctgtt
     6421 cctttcatac tgtttttatt atcgttcatc cgctgacggc aggcgacacg cggtcacgcc
     6481 gccatcagcg gacgaatcgt ttggtgaggt acggcggcat acgctatttc acggcaccgg
     6541 tcgtcacacc cttcatgatg ttgcgctgga tgagcatgaa gaacaccacc accggcaacg
     6601 agaaggtcac cgaagcggcc atctgcccgc cgaaatcggt gcccatggtg ggtgtcgaga
     6661 aggacgacag ccataccggc agcgtgtact tcgactggtc cttcatgaac gtataggcga
     6721 tcatgtagtc gttccaggcg ttgatgaacg cgaacaccga ggtcgagacg atgccgggcg
     6781 cggccagcgg gaaggtgatc ttccacagga tctgccagtc gttcgcgcct tccacggccg
     6841 cggattcgaa gatgtccttc gggatgttca ggaagaaccc gcgcatgttc cagatcgcga
     6901 acggcagcac catggcgatg tacgccagga tcaggccgct gtatttgttg agcaggccga
     6961 gctggttgta gatgatgatc tgcgggatga tgccgccaga ggtcatctgg atggccagca
     7021 cgaacaccat gatcggcttg cgcccgcgga agcggtacag ggtcagggcc gtgcacgcga
     7081 agaacgcgac aataatcgac aggacgatcg agaggtgcat gggccagaac acgggggtcg
     7141 tggtcatgat ctcatcgcgc ggctttacgg cggtgataat catccagtag accgggaaga
     7201 accatacgac gcagaacagc agggcgagga tagtggtgcc ggcgcggcgc gcgatcttgc
     7261 cggggttggt tcggcggcgg ggcggtgcag actgcgctgc gccatgcgat acggcccgtg
     7321 gattctcaac acatttcata aggacgaccc gctatttcag ggattcgcca atatcagcgt
     7381 gccagtcgcc atttccggcg tcggctatgg cagcgccacc ccttccccac cgtcgacatc
     7441 gacaaccgac tcgcttcacg caagctcacc gagcatatgt tggggcgcgg gtatcgacga
     7501 atcgcctata tcatcggacc gtcgtacttg ccgacgtcga cgccgacgcc agcgtgcccg
     7561 cagctgacca ccgtgcgcca gccaatggac gcattcggcg tcagcttggc taccctagtg
     7621 cttgaccaga tcgaggaccg gccgttccag cgcacggggc tcctgcccac cgaggtcgta
     7681 gccagatagg cgcgcagaga acgggtctca ataatgtctg tgcacacttc tctacgcccg
     7741 aagcacacat ggaatatgcc ctaatcactg ccatgtcgcc atttgcgatg atgtgagatt
     7801 gttatgttct tgttcatttg agcgcacaga tactactgag aaccgttctc tgcgcaaata
     7861 gacaatgtgg ataactccat aagctggaac cacattgccc tgcctggctt gcattcaagt
     7921 ccaaatcagc tttacggtcg caccatgagc tcttttatgc atgaggatat tgtcggccga
     7981 cgccgagcag aacgtgaacg ctgcaatgat gctgcgcgcc gttcgaaagg tccattgctg
     8041 gtgggataca tcaatgcggc caactactac ggcgcgccta tcccccgaca ttgcacgcta
     8101 tctgttgatg ccgtgcacgc ctgtgtttcc agtgccaaca aacgcagacg aatcaaaggc
     8161 gtggtcttcc atgtgtttaa gggaaaactg gatatccgca aaaaggatta tgaacagttc
     8221 tggattgcct cgccggtcat gatgtgggct cagatggccc aatactctac attggaggaa
     8281 cttgcggcca ttggagcctc tttgatgagc cgcgataaac ggcgcagaat ggccactcgc
     8341 aaggatttcg atacttactt ggaaatcagt ccgcggttca taggtcgtaa caaatgccac
     8401 gaggcactgc cctacatgac agagaacacg gattctccgc cggaaaacac gctgtttggg
     8461 atgctgaagg attcaggctt gggatgcccc accgccaact atcgcgtgaa tatcggcaac
     8521 tcatacgtca tattggatat ggcatatccg gactgccgcg tcgcgttcga atatcaggga
     8581 gcctatcacg ccgatccggc acaaatgcga atcgacgcgg caaagcgaaa cgcattgcaa
     8641 ctgctcggct ggatcgtcat tctcgtcact gcggatgacc tgagaacagc cgacacgcgg
     8701 catcgtttta tcgagatggc ccatgtcgtg gtgtcccggc aacgcaatct cgccgattgg
     8761 aaccgctcat gggccattac ctcttagcat cgcaccgaca atgcgctatg ccgttattta
     8821 ctgcgaggca cccgccagat atcgggctca acgtagatct cccattcata ccaaggcagc
     8881 gcggcacggc gtcctcgtcc atgtgaatgg cctgcaggtt aatgagccta tccacgccga
     8941 cagtgcgctc cattgcctcg accaccaggt cgcgggtgct gtggtcaagc gattcgccca
     9001 ccagcagcga accggactta aacgccaacg ccgcctacaa aagtgtgagg ctgcgcaggc
     9061 tcacactgcc aacagccgtt cagccaaccc tcggaaggtc tggccaatgc catccgtacg
     9121 caaagcgccg tcggcgtcca gcaccgccgg ccggccggcc tcgccggtct cgcgcacttc
     9181 gggtttcagc ggcagctggg ccatcagcgg cacgtcatag cccagcgcct gagtgagctg
     9241 ttcagacacg cgctgaccgc cgccggcacc gaaaatctcc agcttctcgc ccttgtgctc
     9301 gtagtagctc atgttctcca ccacgccacg caccttcatc ggcacctgca gggccacgag
     9361 tccggagcgc acggcgatgt cagaagcgga gggctgcggg gtggtgacca ccacgagttc
     9421 ggcgttgggc agcgcctggg ccacggaaat cgccatgtcg ccggtgccgg gagccaagtc
     9481 gagcaagagg acgtccggct cgccccacca tacgtcagac aggaactgtt ccaaggagcg
     9541 ctgcagtcgc ggcccacgcc acagaatcgc ccggtcagca ccggcgaaca tgccgatgga
     9601 gatcagtttc acgccccacg cggtgaccgg catgagcatg ccattcagat tggtgggctg
     9661 tgtatgcacg ccgaacaggc gcggcagtga gaagccataa atgtccgcgt cgatggcggc
     9721 cgtgtcgaag ccgagggccg caaacgtggc ggccaggttc gcggtaaccg aggacttgcc
     9781 gacgccgccc ttaccggagg cgatggcgaa gatgcgggtc ttgacacccg gtttgctgaa
     9841 ggggttctgc ttgcgttcgg ccttgaggtc ggcgaccaga tcggccagct tgtcacggct
     9901 catcgaaccg acttcgatat gaggcagcag ctgtgcgccc ggataggagc tgaccgcgcc
     9961 attgatctgg ctggtgatgg tctcgctcag tgggcatccg ggaaccgtca gctcaacgtg
    10021 cacggtgacg tcgtaagtgc cggcgtccga cgatgcgggc gcggcttcga tggcggcaat
    10081 catgccgagg tcggtgaccg aacgaccgag ctcggggtcg atgaccttgc tcaggcgttc
    10141 gtagatgtcg gcttcgatct gacgtgcgtc gctcataaat ccgtatcctc ctttagggcc
    10201 tgttctaccc tagcgtgtga tgcggcggca tgccagaggg agtggagtaa gtcacactgg
    10261 ggcgggattc gctttttctc tcccccagtc agcctaaagg ctgacagccc cctcatcaga
    10321 gggggttaat gaagcgtctg agggggctat tttagatggt gccggtggct aggagctggg
    10381 cgaattgggt ttcgctcagc atggggatgc cgagttcctc ggccttggcg gccttggagc
    10441 cggcgtttgc gccaatcacc acgtaatcgg tcttcttgct caccgaaccg gccgccttgc
    10501 cgccgcgctc gataatcgcc tctttggcgg aatcacgcga atagccctcc aatgaaccgg
    10561 tgaccaccac ggttttgccg gccagcgtct gcggcaacga actggtctcg gcctcgtcga
    10621 cgcccacgcc ggcggcctgc caagcacgca aagtcgcgcc gcgccagtcg ccgggttcgc
    10681 gggtggcggc gaaccagttc accaccgatt cggcgatttc cgggcccacc ccgtcgatgg
    10741 ccgtcagatc ttcaatcgtg gcgttttcga tggctgccag cgagcccatc gccgaggcaa
    10801 tcaggcgtgc ggtaggcggc ccgagccggc gaatcgacaa cgcgaccagc acacgccaca
    10861 ggtcggcgtg acgggccttg tccatttcgt cgaacatctt gcgcgtgttt tcgccgggcc
    10921 gaatgatcac cggtacatcc gtgacgccag tgcgcgtggt cttgtgatcc acgcgcacga
    10981 taaccgcgtc ggccggcacg tcatactcag gataggaggc tgcggcaaag cccggcatag
    11041 taccaagtgg cgcctcagcc ccggtaggtg cgccggtcgt ctcgctggcg gtacctccct
    11101 gtgtttcggc agattcgatt gcggcctccc cttgcgcgcg ttcggccagc tgcttggccg
    11161 tgagcttttt ggccggcgtg ggcgcggtcc agaaagcagg cacctgatgc cacaggccag
    11221 agccgccgac acgcttacgc acctttttct tcttaccgtt cgcaccgacc gtctcatgca
    11281 cctcgacgat tgcggcctca cgccacacgc gcacgtcgcg cagatcggcg gcggtcaggt
    11341 tgaacaaccc ggattcgttg gacaacaccg gcttctgcgc ggccggcaat tccagccctt
    11401 ccaccggctc gtacgggtca ggctcttcgc cgggggcgac cagaacctcg gtgatgttcg
    11461 gcgcgtaggt cgccaccgaa tcaggccggt tctcctccgg gttggtcaga gcgatggccg
    11521 attgttcgcc caagtgcgcg atgtcgaacg ccttgcgtga ggccagcgaa gtcacgcgct
    11581 cggtcaactg ggccgggcag ctctccacat tcgggcagcg aatgtccttg tccccctcct
    11641 tggcgggtga cagtttcgcg ccgcatgaag ggcagaactc gggcatgacg aattcgcgca
    11701 gctggtcctc acgccctttg cgccgttcaa gcaccgggcc gaccagttcg ggaatcacat
    11761 cgccggcctt gcgcaccacc acggtgtcgc cgatcagaat gcccttgcgt ttgacttcaa
    11821 atccattgtg cagcgtggta cgagccacgg tggagccggc cacgtagaca ggtttgagca
    11881 cggcgaccgg cgtcacacgg ccggtacgcc ccacctgcac ggtgatgttg agcagttcgg
    11941 tgttgacctc ttcgggcggg tatttgtagg cgatggccca gcgcggcgcg cgcgaagtgg
    12001 cgccgagggt acgctgcagg cccaggtcat cgaccttgac gacgatgccg tcaagtgcat
    12061 gctcgatgtc gccacgatgc tcgccgtagt actcgatcat gtccaggatc tcctggaatg
    12121 aggtcaccgc acgattgtgc ggggagaccg gcacgcccca cttggtgtat agatcgtagg
    12181 cctgcgactg gtcggcgacc acgtcatggg tgccgcgcgg atggtccggc ccccaagtga
    12241 gctgccccag accatgcgca tagaagctca ggcgacgggt ggcggtgatg cgagggtctt
    12301 tttgacgcag cgagccggca gccgcgttgc gtgggttggc gaagggcgct cgtccggcgt
    12361 cctcctgctc gttgttcagg gtgtggaaat catcccaacg catgaacact tcgcctcgga
    12421 tttccacgaa gtccggaacg tcttcctcnn nnnnnnnntc ttccttgggg ccgccgaggt
    12481 ttgcgggaat ggagccgatg gttcgcacat tcagggtgat gtcttcgccg gtaacgccat
    12541 cgccgcgcgt caggccctgt tcaagcacgc cgttgcgata gatgaggttc agggcgaggc
    12601 cgtcgatttt gacctcgcag ctcatgggca ggggcttgga ttcgggccaa tcgaggtcgc
    12661 gaatcaccga gtcgtaccaa tccttgagtt cttcgatgga gaagacgtcg tcaaggctca
    12721 tcatgcggct ggggtggcgc acggaggcga agtcgttgga gaaggaaccg ccgacgcgat
    12781 gggtgggcga ctgcggattg tccagtgagg ggaacgccgc ttcgagtcgt tcgaggcaac
    12841 gcatgcgcgc gtcgtaggcg gcgtcggagc tgacgggtga gtcgtcgatg tagtaggcga
    12901 tctgatcgga ttccacccat gcggccacgc gagcccacag gcgggcggcg gcctcagcgg
    12961 tcatcgtgtt cacgtcgaaa cggtcgaggc gtatcgcgtc gtcgtcagtg ggctgcagtg
    13021 cggctatcca ttgttcggag ccaggggcaa aacgtgcaat accggtatca ggccggacct
    13081 cggccacatc cccatcgtcg aaatcccatg ccagttgttc gttcgtgctc atggttgtct
    13141 atcctagcgg gctcggctcc cctcggccgg ctataccgtc aactcccctc aggcggctac
    13201 gcggatagcg agcgcaggaa ctgggcttgg atgatgtcga tgttatcgcc ttcgccgtca
    13261 ttcatgcggg ccacagccac cgagagcgtg cgggccggca cgaacatgtc ctcatccacg
    13321 cacacgcgag cggcgtcacg cacgatgtca gccacttcga tatgcggcca gaccggcaga
    13381 tcatgcgtgg caagcacatg ggcggcggcc tccaccgatt caggcaccgg tacgcactgc
    13441 gagtccgcac gggaaaccag ttcctgcagt tcgctttcca gcgaaccaat ggccccggga
    13501 gcgatgtgat cgctcataat gtaggctgcg gcggctgtgt cgaaccggtc aagcatccat
    13561 tcggcgtacg cgaaccggta ataggcgaac gcggcatcgt cgcggtccaa ggccacgcgc
    13621 agagcattca ggcaggcggc gcgtgccgaa tcccaatcct cattgcgggc cagttgaatg
    13681 gcgagcttca gatgtgagag cgggtaggcg ggcgcgtacg ccaccatacg gttgagatgc
    13741 gggatggccg cctcggcacc cttgatctga ctcaacacgt cagccagctc catgtgcgcg
    13801 tagaacagat tgtccgggat aagcaccgta cgctcgtcca atgtggcgaa cagccggttg
    13861 tagaccacgc gctcggcata ggagttgaag tatcgaggta cgccatggcc tgccgcatac
    13921 acctggtcca aatgggccac ggccgcttcg gccgcttcga tggcctgatc ggcttggccc
    13981 gagaacagca tatctcgggc gcgcagacgc tccttgtcaa gatcgtcggc aagcgtgaac
    14041 gtgaagtccg gcgtgtcctt gccatcgatc aacgcactgg tgacctgaga ggccaaaccg
    14101 gtcagttcgg gatcgcatat cgattcgatg acctgcatgg ctcgctgggc cttttccacg
    14161 gagccgagcg acgtgttcct ggcgatatcg tggaattcgt tgacgccatg ctgcagcagg
    14221 tccactcgct ggatagccag tccggcacta ttctgtgcgc ccagcacgtg agcggtcgcc
    14281 ggactgaatt gacggtcggc cagttcgggc tcctcctggg atccggtggg agagaaccgg
    14341 ctgtccgcca agtcgaagtc ggcgctgatg ggtttcagac ccccttcgcc gtccacgtcc
    14401 atcacggcac cgaacatgcg gtaggtttcc tcgggattgt caagcccagc ggtgtccagt
    14461 cgcttgagga aggcatcacg ggtgaaggtg acggtgacca tattgcgcac ggtgggattg
    14521 cggcgcagca cactcatcgg atcatcgtcc atgccggcgg ccccatcagc accgtcagtg
    14581 ccgacgccct cgccgccgat accgcccagg tcgtcgcctg cgccagtatc gtccggcatg
    14641 ccgaacgcca tttcgtcgat atcgatgccc ttcatcaggt cttcgaactg cgaatcgatg
    14701 gacgcagcgc catcggcgtc agcagattcg gccgcggcct gatccgtagc atccgcagac
    14761 ggtccggtac cgttgcctgc tcccccggcc gtgccgcgtt cgtcaccgga cgccgtctgc
    14821 ccgaacgtct cctcatgggt gatggggcgg gtcgggtcgc catggaaatc accatctttg
    14881 gggtcggctt tggaacccac acgccccaaa tcgccactgc gtagatgttc gaaggcgctt
    14941 agggcctcgc tcatcatcgc ctcgatggcg gaatcctgtt cggcaatcgc ctcttcaaga
    15001 ccaatggaat cgatgtgcaa cgacacctgg cgcacggatt cgctggtgcc gaaactcagc
    15061 gctgccatca tcaggccgac acgcaggttg taggcggcgc tcatcgacgc gcgttcggcg
    15121 ctgctcagcg cacgccacgt acggcgagtg ctgtcatacc ggctatcggg catcatcgag
    15181 gtgccggcgg tggtgaagcc aatggcccct tcacccgaat ccaagcgcga ccggaactcc
    15241 acgtcaaacc gatacggcaa ccgtaaccgg cgcaacagtg aagagagggt ctgacgatac
    15301 acccattcgt cgctcctcgc cggcgaatac gaaggacgag cggcttgagt gttggtgtcg
    15361 ctcttatcgg tccgttctac agcgccctca gcactctcag tactttcagc gcccgcagca
    15421 ttcgtcacac ccgaatccgg cacgttctgc tgggtcagca catctcgagg atcggggaat
    15481 tcactgcgaa tctgtccagc cgtgcgtgcg gcgatatcga ccaggctcag cacggcccga
    15541 cgctcctgct cgccctcata c
//
LOCUS       DAWBMW010000020        15555 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_964_length_15555_cov_2.832710, whole genome shotgun sequence.
ACCESSION   DAWBMW010000020 DAWBMW010000000
VERSION     DAWBMW010000020.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 15555)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 15555)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15555
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_964_length_15555_cov_2.832710"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    861..870
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1831..1840
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5276..5285
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7203..7212
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    15292..15301
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2988 a         4447 c         4890 g         3180 t
ORIGIN      
        1 cgaatctccg gctggttctt caggtagaag tcgatgtagt agtcggtcat ggtgacgaga
       61 aagtcgccgg cctgcgtccc cagagacgcc cattcatggg ctttctcacg agtgagcgcg
      121 gtctggtggg tcacgtcgag gccgatcatg gtggtgcgtg cgccacactt gaacagatag
      181 tctactgcct ctgggtcttg ggagatgttc gcttcggcgg ccgggcttac attgccgggc
      241 aatgtgaggg cgccgcccat caacgtcacg ttcacaccgg aatccttcag gtccggagcg
      301 ttgcggaata cggtggccat ggtggtcatc gcgcccgtag gtacgatggt caggtccggg
      361 ccgtattcgc gcgcggcggt gatgatgaaa tcgaccgccg agcctggtgc ttccggagcg
      421 cgcggggagt cggggatgat tgcgccgccg atgccgttgg aatggtggac cgccaccgag
      481 ccgggagttg gtgaccacga agcgtccgtt tgccagccgt ccgcgccgcc gttctgccag
      541 cgcgcgtagg cctgggcccg ctcgtctggt gtggtgccac gcattttcgg caatgttgcc
      601 ggggcgatgg gatggtcgac tccgggatag accggaacgt cgtcacggcc gaacaagtgc
      661 agtaccgcca gcgcgttacg cgcggccagc ggcaccgcga cattgccgta ggtggccgtg
      721 ataccgaaca aatcgatctc gtcggcactg cctagggcat atgcgatggc gagcgcgtca
      781 tcgatgccgg tatcgaggtc gaggatgagt tttttgcgca caattatctc cttgtggaac
      841 tacccgggac tactccggcg nnnnnnnnnn gaggatggtt tcgggacggt gcgcgccggg
      901 aatcacgaac atgtggtcgc ccttggcgag ctcccatgcc agcacgacct gctggtagct
      961 cacgccgtgg gccgccgcga cctcgcggaa cgggtcgaac ttggtctcat cataggggtg
     1021 acggtagccg ccgagcggac tccagcacac gaaggccaag ccaaccttgg cgcagtagtc
     1081 gagcgtgtcc tgggtctcca ggtgaatcgg cgagtactgg ttttgcacgg ccacgagctt
     1141 gtcgcccaga ataccgcgcg caatgtcgat ttgctcgatg gaggcgttgg agataccggc
     1201 ctcctgcgcc acgccctcgt ccaccagctg cttgatggct tcgacggact cgttgtacgg
     1261 caccttcgga tcagggcggt ggaagtacag caggtcaatg gtgtccacgc cgagcgccag
     1321 tgcgctctcc ttgccgcgcc gaatcaggtt ctccgggcga ccgtccacat cccacgtggg
     1381 gcggccgtcg gtgaagttgc ggaagtggcc caccttggtg gccaccagca cctcgtcctt
     1441 cggccccttc caggtctcaa gggcctcgcg caccagcttt tcaccggtct gttcttcgcc
     1501 gccggagcag tagtaggccc aagcggtgtc gatgtggcgg cagccggcgt cccgcgaggc
     1561 atgcagggtt tcgatgccga cctcgtggcc gcgattgttt tcgatggtca gcggcatttc
     1621 gccgtggccg atggcggtgg tggagaacgg tccgaggtta cggaacaatg ccatggtgat
     1681 tcccttcaac agtaatgaga cggtctgtgt ccgattccag cgtaagcaga acgctggatt
     1741 taccgcccac cgaacgagcc gccacccgaa ccgccgaacg agccaccgaa accggagccg
     1801 ccacccgagc cgaagctgga gaaatcgcca nnnnnnnnnn gttcagctgc gagccgaggt
     1861 cggagaatcc gccggcaaaa gatgtgccta cgtaagccgg agctgggccg ctaatgcccg
     1921 ggtccatctg cccggcagca tcgtggtcga acggaccatt atggcggtgg ctgtaccagc
     1981 catacgggcg gtagttccag tagaacaacg agttggaggc gttggcatcc agccaagccg
     2041 gatcgttgac ctgaggatag gccttggcca gctcacgcat cacgcgatcg gaaatgccaa
     2101 acgcggcggc gtagaccata taccaatccc acaaggccaa atcggcggca ccacggtcgg
     2161 tgaagttgga gaagtcttcc atataacgct taaggcccag agtccggccg gcaatatcct
     2221 ggccttggtc ggtgagcacg gtcatcgcgc cggccatcga gcagaacagg ccgaccagga
     2281 atatcggcag ctcgataaga ccagccaccg ggtatccgat aaagctgttg gccagcagcg
     2341 aaccaaatcc cagcacggcg gccaatacgc cgggtaggat ccattgccag cccacagagc
     2401 gcgtggcacc cagccgctgg tattccatgc tgcatgcacc ggtgaatttg ccgagctcaa
     2461 tatagccgtc ttgccaattc tggcaggtcg ccttcatctg gttcaggtcg aataccgggc
     2521 tgcccacgcg ctgcgaaatg acgatgagca ggttcagcag cgcatcttcc gattcgctca
     2581 atcccaactg ttgcgcgttg ggtgcctcat caatggcgag cggcagaatc acgatggtgc
     2641 tggtgatgcc tgccgcatac tgcttgcctt ggtcggcggc aatcatttgg ctcaagccca
     2701 ccggcgtggc ctggctcatg tcgatgccgc gatacatgtc cgacggcccc ggatagacgg
     2761 caatggcctt cttgacggcg agcgacagca tggtggcggt cagctggcgg tcttcatcgg
     2821 attggcgcgt cgacggatcg accacgcgaa tcagacgggc tgcggaagcc ggactgatgc
     2881 cgggctggtc gcgccagtat tcgacgctgc cacggtattg cgcacgccgg ttgctggaga
     2941 tcacagccca gataccccac gcgcacaacg cgatgccgag cacgatgctg acaatccaga
     3001 acacgagacg agctctggca gcgaatcgtt gcttgtcgag ccagcgctgc tgttgcttat
     3061 attcgtcctg tttcaggtcc ttgagatgat tgccggttcc cttgcgggcg atgcccttgg
     3121 ctttggcggc atcgaacgcc gcgaccacat cgaggtagtc gccattgtgg atgttgtagg
     3181 ccgtgaacgt gtagctgccg tcgctgttgc gcttggtttc cgaagtgcgc tcggtgtgca
     3241 gccatgccca tgaggttttg ccggtgatgc cgttggggaa gtggacagtg ccggtgaccg
     3301 tgccgatagg cacttggttc ttcttgccga acggctccca ttggaaggag gccacgtctt
     3361 tccatttggt ggccacatcg tgcatggtga agctcacgtc gaacttcata ctgtcggcct
     3421 cggtggtgac cggaatattc cagccgatct ccacggtcgt gtcatcttcg gtggcggagg
     3481 cgctgggctt gaggccatcg gtaccgggcg tgtatgcctt tggatggtcg ctgccatcgc
     3541 tcacatcggc gacataccaa tggttggcgt agtcgctgtt ccattccttg tcggaagaga
     3601 cgtcactggg cagcttgggc tcgctctgtt gtgcgtagtc gatgccgtcg gtgacgttgc
     3661 gcacgctgat atcgctgata tcggtcaggg tgccggagtt cagcgtgtat tgctggaaca
     3721 gttgcttcca cggcttggtg ttgtcgtcgc tgtcggtacg ctcacgcatc ttgacgtcga
     3781 tatgctgcgt gaccttcaaa tcaccgttcg cggtggcggt cacgtcatag tccagcgtgc
     3841 ggtagctgag gtcggccgaa tcgccgaacg cggaagtccc gattatcgcc aaggtgataa
     3901 ctgccaacac caccgtggca acaatgctga tgattccggc gcgaatgaat cgtatccgca
     3961 tgtcttccct ctcttaagac aatacgggcc cggccggcgg cttaccggac gggcctgaag
     4021 cgggtcagaa ctggacctgt ggcacctgac gggaagcgtc atcggcctcg aagtattgcg
     4081 cctgctcaaa gtggaacaga ccggcgatga tgttcgtcgg cacggtttcg atagcggtgt
     4141 tgagcttcag gaccacgtcg ttgtagaact gacgggcgta ggcgatcttg ttctccagtt
     4201 ccttgagctg gttctgcaga tccatgaagt tctggttggc ttgcagctgc ggatatgcct
     4261 cagccgtggc ttgcaggttg aacagggcgc gggacagctg gttttcggcg gcggcgcgct
     4321 gagcagtggt cgtgctcggg ttgctggccg cagccactac gccggcgcgg gcggcggtca
     4381 cctgagtgaa gacttcagac tcgtgggatg cgtacccctt gacggtattc accaggttcg
     4441 ggatgaggtc ggcgcgctgc ttcaactgca catcgatttg cgcccaaccg ttcttcacac
     4501 ggttcttcag cgtcaccagg ttgttatagg cgctgacgcc ccaaccaatc aagacaacga
     4561 taataacgac aacgacaata ccgacgatca gaccagtact cacctatgct cctttgctgg
     4621 accttgctgt gaccattctt ccacactcgc accgtaaaca gaagattcat cacagagtat
     4681 tcatcgtgcg gacttgtctt gtaccgcttt acccgccgtt ctcccgtcga tcgaccgaag
     4741 cgggaagtcg ccgcccagtc cgacaaagta ttccagctcg ctccgttgcg agaacgataa
     4801 tactcggcaa tactcgcgtt gaatatgcga aagcccgcct tgcggcgggc tttgccgttt
     4861 ctcctacgca caaatcatta cgctgcgcgc aggaaacgat cagccgaacc ttccagagat
     4921 gtagcgctcg gcttcttcgt tttgcgggtt gttgaacatc gtgatggtgt ccgcgaagta
     4981 ctcgaggtga ccgggctggc ccactgcctt caggttgaag aaggcggtgt agtcggcgat
     5041 acgggcggcc tgctgcatgt tgtgggtcac gatcacgatg gtgtagtcgc tcttgagctc
     5101 gttgatcaga tcctcgacag ccaaggtcga aatcgggtcc aaggcggagc agggctcatc
     5161 catgagcagc acctgcgggt ggacggccac ggcgcgggcg atgcacagac gctgctgctg
     5221 gccaccggac aagccgatgc cagggttgtc aagacggtcc ttgacctctt cccagnnnnn
     5281 nnnnncccag aggttggcac cgcgcagagc ccactcgacc agatcgtcgg cgtcggactt
     5341 ggccaggtgg tggttgttca ggcgcacacc ggccagcacg ttctcacgga tggacatggt
     5401 cgggaacggg ttcgggcgct ggaagaccat gccgacgtcg cggcgcacgg ccaccggatc
     5461 cacgtccttg tcgtacaggt tcttgccttc gagcagcacc tcgccctcga cgtgagcgcc
     5521 agggatgatc tcgtgcatac ggtccaaggt gcgcagcacg gtggacttgc cgcagccgga
     5581 cgggccgatg aaggaggtga ccttgttcgg ctcgatgttg atgttcacat cttccacggc
     5641 cagaaagtcg ccgtagtaga tgttttcgtg gttgacgtca atacgttgtc ccatgatgtt
     5701 tccttatatt tccttgtgtt cttaaggctt aggcttagcg actggcttag cgttcggtct
     5761 tgacggcgaa gaccttcgcc acgattcggc caatgaggtt cagcagcagc acgatgacga
     5821 tcagcacgag agccgcagcc cacgcacgtt ccatcggaat cgtggtcacg caggtggcgt
     5881 cggcgttggg cgggcagttc gcgctgagct tcgagtactc ctggtagacg aacaccggca
     5941 gggtggtcat ctggccggag aacaggttca cgttggtgct ggagatgtag ccggcggtca
     6001 tcagcaacgg tgcggtctca ccaatcacgc gggcgatggc cagaatggca ccggagacga
     6061 tgcccggcag agcggtgcgc agcacgatct tggtaatcgt gcgctgcttg gtaacgccca
     6121 gagccaagga agcctcacgc aaatcgttcg gcacgatctt gagcatttcc tcgctggact
     6181 tgaccacggt gggcaacatc agcagggaga gagccacgga gccttcgaaa ccgttgatgg
     6241 taccggggcc cagcagaatc gtgaacatcg agaaggcgaa caggccggcc acgatggacg
     6301 ggatgccgct catcacgtcg acgagcaggg agaccgtggt ggcgagcttg ccgcggttcg
     6361 agtattcgac caggtacacg gcgcacatca aaccgatcgg aatggagata accatagcgc
     6421 cagcggtgat ttcgagagta ccgatgatgg cgtgcagcac accgccgtaa ccaccggacg
     6481 gggtctggtt accaccgacc acaccggtca tgttgtagct caggaagttc aggttcaggc
     6541 gcttgatgcc gttgacgata gtggtccaca gcagggagat cagcgggatg caggcgatca
     6601 caaacgccac cgcgatcagg acccacatga agtggtccat gatattacgg cgctggatgg
     6661 cggaacgggt cgggcggaaa cgatcgaagt cgatttcggg cgcgccgtcc atcggagtct
     6721 tttcagtgtt ctcagcagca gtcatcacgc agtcgctttc tcggtaagct tacgggcgat
     6781 gaagttgacc acgaacgtga tcaggaacag aatcagacca gttccgatca gcgtggagac
     6841 gcccagatcg ttggcttcag ggtactgtgc ggcaatattc gcggcgatgg tctggttctg
     6901 cgaggcctgc agcagcttga tggagtagtt gaggcccggg gacaggatca tcaacacggc
     6961 catcgtctcg ccaagcgcgc ggccgagagc cagcatggag gcggacacga taccggactt
     7021 gccgaagggc agcacggcca gacgaatcat ctcccacttg gtggcgccga gcgccagcgc
     7081 ggcctcttcg tgcaggcgtg gggtctgcat gaagatatcg cgggacatgg aggtgataat
     7141 cggcaaaatc ataacggcga gcaccacagc cacggtggcg acggtgcgcg gcgggttggc
     7201 ggnnnnnnnn nnaacagtgg aatgaagccc aggtggttgg caacccagtt ccagaacggg
     7261 tagatggccg gcaccagaat caggccgccc cacaaaccgt agataacgga cgggatggca
     7321 gccagcaaat cgaccacata gctcagcgcg gtggcgagct tcttaggcgc ataatgcgaa
     7381 atgaacagcg caatgccgat ggacacgaag aaggcaatca gcagtgccag caaagacacg
     7441 agcacagtac cgaacaacag cgggagcacg tagccccaga agctgctggc cttgccgccg
     7501 gtaaagctgg acacggtctg gctgttggcg gcctgatcgc caccgatgag tggccaggcg
     7561 cggaagaaca ggaacaggaa cacagcggca agtacaacca ggatcaggat gccgcaggcg
     7621 tatgccacac cgcgaaacac cttgtcggcg gtcttgccac caactggctg cacagccgtt
     7681 ttgtcttgcg aactcacgga ttctccttat atagggtggt tatcaagtat tcaagtggtg
     7741 cggttttcgt ctgtgacgtt gccaaaccgt tcgggtcctg acccgcttta agccgcagcc
     7801 gtatggttta cgactgcggc ttaaagttgg cggcgactgc tcttactact acagtctatc
     7861 gttcagttgt caggtccagt ccacacgacc cttgcgggcc gacgtcatca cttggtcttg
     7921 atggcctcga tggacttggt gatctcggac ttcagggagg agggcagcgg agcggtgccg
     7981 gcggcatcct gagcggcctt ctggccctcg tcggaggtca cgtaggtcag ccaagccttg
     8041 gcgaactcag cctgcttggt gtccttgtag gccgggcaga cgatgtcgta ggagaccagg
     8101 acgatcgggt aggcgccctc ggcctcggtg gcgtggttga tcttgatgac gatgcggttg
     8161 tcacccttaa cggtgtcgtc ctgcttggaa tccccaatga ccttggagcc ggcctcggcg
     8221 gaaatctcgt tgtacttgtc gccgaccttg acggccacgg tgcccaggtc gccgacctga
     8281 gagaagtcag cgtagccgat ggtgccgtcg gcctgcttga cggtggagat cacgccagaa
     8341 gtgcccttgg caccctggcc aacctcgttc ggccagttct cggagaggtc gtaggtccag
     8401 ttgtcagggg tgacgtcctt gaagtaggag acgaagttct gggtggtgcc ggacttatcg
     8461 gagcggtgca ccacggtgat ggcggtgtcg ggcagcttca ggtccttgtt ctgatcggcg
     8521 atggccggat cgttccactt ggtgatcttg ccgtcgaaga tcttggcgat ggtggcagcg
     8581 tccatgttga tgtgcttgcc ggcatcggaa acgcccttga ggttgaaaac cacggcgatc
     8641 ggggagatgt agaccgggac atcgaaggcg gtgccttcag tgcacacgga cttggactgc
     8701 tcaacttcgt catcggcaag ggccttgtcg gagccggccc aagcggtggc accggtcagg
     8761 aaggtctgca cgccagcgcc ggaaccggac gggttgtagg cgatcttggc ctcagggttg
     8821 gtgccctgga agccggcgat ccaggcttca acggcggcct gctgagagga ggcgccggcg
     8881 cccgagaagt tgccggagat cggggtggac ttggaagtgg aatcggagga ggagctgttg
     8941 tcggtcgtgg tggcggtgtt atcgccgcag gcggccacgg aagcgagcat cacaatgccg
     9001 gacagagcgg caatggagcg aacgaggatg ttcttctgca tgattattac ttaatccctc
     9061 tatcaactat tcgcgggcgg ctcagcgctg cccggtcaat tcatgacaac tacaagcgta
     9121 ttggtggttt caagggccca aaaccgaaat cagtaaacag aagatgaaca cttggatgtt
     9181 tgtgggctac gttgaggcta cgtagttttt ggagcgggct cgcctgcggc gagtccacag
     9241 cgagcccaca atccacaaca aaaaaggcga cccgaaggcc gccttccatt tactccgcgg
     9301 gagtatcgat cttgtatccg agaccgcgca cggtagtcag atacttgggg tgcgccggat
     9361 cttcctcgat cttggagcgc acgcgcttga catgcacgtc gagcgtcttg gtgtcgccca
     9421 cataatccga accccagatg cggtcgatca gctgatgacg cgtcatcaca cggcccttgt
     9481 tctgcatgag gtattcgagc agttcgaatt ccttgagcgg gaagaacacg gtttcgccgc
     9541 gtacggtgac ctgatgctgg ccaacctgca tggcgatatc gccacacacc agcggaatat
     9601 cgtcatccac agtgccggaa gcctgggcag tggcctgatt gcgacgcatc acggcacgaa
     9661 tacgtgccag cagctcacgg aaagagtacg gcttggtcac ataatcgtcc gcaccaatct
     9721 caaggccaac gaccttatcg atttcggcgc tcttggcggt gagcataata atcggcacac
     9781 ggctctgctc acgaatgcgg cggcacagtg cagtgccgtc aatgcccggc agcatcaaat
     9841 caagcagcac caaatcgatg ccgcccttgg tgaacagttc cagaccttcc tcgccagtgg
     9901 cggcggcgga cacgtcgtac ccctcgcgtg tgagctggta taccagcggc tcgcgatagg
     9961 attcctcgtc ttcgacgata agaatgcgtg tcatagtagc ctttcttggg tatgttcccg
    10021 tgcgcatagg cgcaaagttt tccgacatta tcgtaaccat ttgttctccg ttagcctgct
    10081 gtttcccgaa agtacgtaat tgcgtaattg ggctacgcgc ggtgtgcgca cctcgcccac
    10141 tgtgtgattg tgtggcaatg gaatggcacc atgtcgggca ccaaccaagg tcctacgctt
    10201 gtgtcgactc gtccgaccgg gcctcgtcgt catcctcatc cggggcggcc ggcagctcga
    10261 tggtgaacgt cgacccctcc cccgtacgcg accagaccga aatccgaccg ccgttctcct
    10321 gcacgcagtg cttggtgatg gccaagccga gtcccgaacc gccggtctcg cgcgaacggg
    10381 ccgggtcgac ccggtagaat cgctcgaaaa tgcggtccag cgatttggcg gggatgccga
    10441 tgccctgatc gaccacgcga atggtgactt tgccatcgcg ctcccccacg ccgaccgcca
    10501 cagtggtgtg ttcgggcgag taatggatgg cgttctctac gaggttcttg acggcggttt
    10561 gcatggcctc tttgtcggcc ttgatcatcg gaccgttcgt ggctgggctg cctgattctt
    10621 ccaaggaacc ttcgccatcg gccggctcct cgaccttcgt gggtactgac tgtccgttga
    10681 cactcagtcg gatatccaca tggcgggagt cggcttgggt ctgattggcg gcgatggcgg
    10741 cgcgtgccac atccagcgcg gaaatgcgcc gcgcatcgat cacgctttgc gggctttggg
    10801 ctttctgcaa gtcgataaga tgatgcacca gttcggtcag gcgcgcggat tccttggaga
    10861 tacggccgga aaaataccgt acggcgtccg gatcatcggc ggcgtcggtc acggtttcgg
    10921 ccagtagcgc gatggcaccg gccggagtct tgagttcgtg cgaaacgttg gtcacaaagt
    10981 cacggcggac ggcctcgaaa cggcgctgct cggacatgtc gttgatgaag atggcgtaca
    11041 ggtcatcgcc gatatcgcca atgcgcacac gcaaatacag cgtgttggac gggcgtgact
    11101 ggccggcctc cagtcctcgg ccattagagg acggatagga attgcgattg accggcagct
    11161 gaacctcacg ctcacgcatg ccaccgtcgg cggcggcctg agtgaggatg tcctcgactt
    11221 cacgcgagtt gagtcggtcg ccggagacta ggccgaatgg cactgagccg gggcttgaat
    11281 attggaccag accgttgcgg tcggtgacga tcagggcttc gggcaggact tcaatcaggc
    11341 gacgctcggt ggggcgcacg ggcgcatcat caccgaatag agaatgcgta tcctcgtcgc
    11401 cggacaggtc ctgttcggcg gcgagccggc ctgaaacgcg gcctttgttg taggcgatga
    11461 cggcaaccca caccagtatc agcgcggcga gcaccacaat cacgacgatc tgccagatat
    11521 ccatgatttc cagagtaaaa cgtgcgaggc gtagtgagat gcctaaacta cgtaagttcg
    11581 tctattgttc atttttggcg ggacgacata acaaaatgct cccgctggcg ggagcataat
    11641 atggggagct ctatttacag ctcgtagtcc ttgtccttgc tttccttgat caggaacaga
    11701 gcgagcagag cgagggcagt catgatagcc atgtaccagc ccacaccggc aacgccgaac
    11761 ttggaggtga ccagttacag gcgcttgacg atgtcgacag ccagacgggc ggcggtatcg
    11821 gcgtattcgg agatgtcgaa ctccttgaat acctcgtaat cggtgtcggc gttgtcggaa
    11881 agggcacgaa tcaccagtgc cggcacgtta ttgcgggcgg cgacctgtgc cacggcggcg
    11941 ccttccatct ccacggcgtc agccccggtg agtcggatga cctcctcgac cttttggggc
    12001 gtgtccacga agtagttgcc ggaggcgatg atgccggtga tgtgggtaat gcccgcattg
    12061 gcaagcgcct cgtcggcgac ttccagcaga cggtcatcag agtggaattc ctccaccgga
    12121 gcgtccgcag tgccgggctt ccactggcct acgagccgca tgtcagtgtc gaggtagcgc
    12181 agggtgccgc cgagcaccac gtcgttgatg tgcaggtggg tgttgaggtt gccggcgatg
    12241 ccgctgaaaa tcacggcgtc cggggcaatc tcgtcaatca gcagctgcgt ggtggcggcc
    12301 gcattgacca gtcccatgcc gcctaccgtt gcggcaacct tgatgacgtc agaaccgaag
    12361 gagtcgaccg tgccggacac gatgtcaagg ctgcccttgg cggtgtgcgt cacatgcgtg
    12421 agcgatttgg cgatcagcgc cacttcctca tccaatgcgc caataatagc tacagttttc
    12481 atacccatac gtaagttcct ttcacaacac tttcggcccc ccttgtcagg ggaggtggcc
    12541 gccgaatggc aggctgaggg gtagagacgt ttcgttgcat tgctcccgcg agagagccga
    12601 tgacgcaact cagtgccaac gccgtgtggc tacggcttcc gcaagctcgc gcagcaaggc
    12661 ctcggtttct tcccagctga tgcacttgtc agtaatcgac ttgccataga ccagctgatc
    12721 aagcggcgcg gccttctgat tgcctccttc aataaagctt tccatcatga tgccggtaat
    12781 accctgttcg ccagctgcaa ttctgctggc aatattgcgc acgacttcgg cctgacgatg
    12841 ttcgtccttg ccggagttac cgtgcgagca atcaacaatc agaccatgcg aggctgcgga
    12901 ttcggcgggc atttcggcgc gtacgtcttc catggctttg gccacggatt cggcatcgta
    12961 gttcgggccg tgaatcgagc cgcgcaacac cacatggcag tccgggttgc caagggtctc
    13021 gactgcgcag gcacgcccca gatggtcaat gccgaagaac gtgtgctgct gagcagcggc
    13081 gaagcaaccg ttgaccgctg ccttcaccga gccgtcagtg gcattcttaa agccgaccgg
    13141 catggaaagc ccgctggcca attggcggtg gatctggctt tccgtgttgc gcgcaccaat
    13201 ggcgccccaa ctcaccgcgt ccgagatgaa ctgcgggctg gtgggctcca gaaattccgt
    13261 ggcggccgcc aatccttcct tcagcacacc aagcaaggtt ttgcgagcaa gcaacaggcc
    13321 cttgcgaata ttgtggctgc catcaatatc cgggtcgttg atcaggccct tccagccaat
    13381 ggtagtgcgc ggcttctcga agtacacgcg catgacgatg agcagctgtt caccgagctc
    13441 atctttcagc ttggccaatc ggcgcgcgta gtccagcgcg gccttcgggt catgaaccga
    13501 acaggggccg acgatgacca acagcctgtc gtcttggccg tacaggcacg cgcgaatctc
    13561 atcacgcgag taggcgacga tttcctgagc ctgatcagtc agcgggagtt cagcgagcac
    13621 ttgcgcgggc gtgggcagcg gctccaactc caatacgcgg cggttgatga tgcgactgac
    13681 acccacctga tcttcccaac gcggcacatc agtggcaacg agcgggttct tcccctcccc
    13741 catcgcctga cgaatggcgc gaagctcttc tggggtgttc aacggctgcg gaccgacttg
    13801 caacggatgc agcgaatgtg ctgcttcctc ctgctgctgc ataacatctc tccttgggtc
    13861 agtatccttc ccaaggataa ggtgtcacgg gaacaaactg acatgaccac cgttattggc
    13921 aaaaaatgct cactgagtgg gtctcagtga gcatttttac aaagacaaag ctatttagcc
    13981 gagcggcgag cggtgacggc gtcggctagg gtgtgcagca gttcgttggt gcgttcccac
    14041 ggcacgcaag agtcggtgac ggactgaccg tagaccaact ggtcgagcgg ggccggcttt
    14101 tggtgaccgc cgaccaagaa gctttccatc attacgccgg tgatgccttg ttcgccgtgc
    14161 gccaggcgct cagcgatctc ttcgatgact tcggcttcgc ggttttcgtc tttgccgcag
    14221 ttgccgtggg cggcatcgat gacgaggcca tgctggctcg ggccggacgc tttggaaacc
    14281 ttgaggtctt ccagagcttg acgcacggat tcggcatcgt agttcgggcc gtggctggag
    14341 ccgcgcagaa ccagatggca atccgggttg cctttggttt cggcggaaat cacgcgaccg
    14401 tccaagttaa tggacaggaa gtgatgctcg aagccggccg cgaaacagga atcggcggcg
    14461 gccttgatgg agccgtcggt ggagttcttg aagcccaccg gcatggacag gccactggcc
    14521 agttcgcggt ggacttggga ttcggtgtta cgcgcgccga tggcacccca gctgatcgcg
    14581 tcgcagatgt actgcggggt gatggggtca agccattcgg tggcggcggg caggccgagg
    14641 ctcagcacgt cggtgagcac cttgcgggcc agccacatgc ccttacgaat attgaagcga
    14701 ccgtccagat cggggtcgtt gatgaggcct ttccagccga tggtggtgcg cggcttctcg
    14761 aagtacacgc gcatcacgat cacgagtcgg tcttccaact cgcgcttgac ggcagccagc
    14821 ttttctgcgt actcgtgcgc ggccttcggg tcgtggattg agcaggggcc cacgatgacg
    14881 agcagtcggt cgtcgcggcc gttgagcaca tcgcggattt cctgccgcga gtggagcacg
    14941 agctcgctca tctcatcggt gagcggctgc tccctgagga aagcacgcgg cgcgggaatg
    15001 gggtcaagct gacgaatgtt gacatcgacg gtctcaggaa agacggcctg ctcccccaaa
    15061 cgacgttcat cctcggaact atccggacca cgtagtgctg ccattgctcg actcctttcc
    15121 ttgtatacgt actgtcggta tgtcgccata gacgcaaaag cccaactata gcagtttgtt
    15181 acggggactg cttgttgggc cggcagttat ggactttggg tggactcgcc tgtgtggact
    15241 cgcctgcggc gatgctgtgt tgttgtgcgg cctgcgaccg cggtctctcc cnnnnnnnnn
    15301 ngagcatggt ggctggctct tcgagggcct tctggtattc ctcgggcaac agggccttcg
    15361 ggacggctac ctcgtagacg tattcggtga accagtcgtc ggacatggtg aagtagccct
    15421 tgtcggcgat cttggcgccc caagagttct ccacgcgcca gcggcgggtg gtgccgtcgt
    15481 cggccacgtc aacgccggcg aaagccatgg cgtggttcat ggcggagtca ccgaagcgca
    15541 cgcgggcttc cttgt
//
LOCUS       DAWBMW010000021        14209 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_1082_length_14209_cov_2.568532, whole genome shotgun sequence.
ACCESSION   DAWBMW010000021 DAWBMW010000000
VERSION     DAWBMW010000021.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 14209)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 14209)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14209
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1082_length_14209_cov_2.568532"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6615..6624
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3019 a         4200 c         4020 g         2960 t
ORIGIN      
        1 gtcgcaacgt gcggccgaag tcggcgaatc gcaagcaatt agcgcaattg cgccggcatg
       61 tgggttacgt gatgcagcat ccagagcatc agctgttcgc tgacacggta gccgaggacg
      121 tggcgtacgg cccacgcaac caagggcttg gcgagaccga ggtagccgac cgcgtgcgcg
      181 aatcactgga actgctgcat atcgggcatc ttgccgaccg ctcccccttc gacctgtccg
      241 gcgggcaaca acgattggca gccattgccg gcgtgctggc ttgcaatcct gacgtgctca
      301 tcatggacga gccgaccgcg agtctggacg cccaagccaa gaagcgtatc catgagctgt
      361 tgcgcacgct caaatcgcgt ggcgtgaccg tgctcatcat cacccatgac cgggaggagg
      421 ccgagcaaat cgccgaccgc gtggtccgta tgccgattgc cgcccccgca tccggcggcc
      481 ccgtcacggc caccgtcacc gagccggccg tatcctccaa cggccctgcg cactcagtga
      541 tacaccgact tgacccgcgc gtgaagatgg tgggcttcct ggccgccatg ttcacgatgt
      601 tcgcggtgaa cacgccaacc cagctcgcgc tgggcatcgc catcaccctg gccgttatcg
      661 cggcggcacg cctgaacccg ttacgggtgc ttgaatccat tcatccgatt ctgatcctgc
      721 tggtgctgat gggcgtggtc aatctgtttg tggtacgcac cggcacgccg gtggtggcgc
      781 ttggcccgct aagcatcacc gatcagggcg tgaccatagc cgtgctgtac gcctgccgtt
      841 tcgcattggt aatcattctg ggcgcggtgt tcctcactac caccacgcct accgccatga
      901 ccgacgcctt cgccaccttg atcagtccgc tcaaccggct cggtatccat gcccaagaaa
      961 tcgcgctggt catgagtctg gcattgcgat tcattccaac gctgaccgac gaaacccgcg
     1021 ccatcgtcga cgcccaatcc gcgcgtggcg gttccatcga aaccggttca ctggcccagc
     1081 gcatcaaggc gatgagcgcg attatcgtgc cgattttcgc cggcacgttg cgtcacgcgg
     1141 acaacctgag cctcgcactt gacgcccgct gctacgagga gggcatccgc cgcacccact
     1201 ggcgtgccct gaccatcgcc gcgcgagatc tcatattcgc cgctgccgtc atcatctaca
     1261 tcgcggccat catcgcgctg tagggcggca aacagcgcag acagcaccgg ttgctatgct
     1321 ggtgtggtta tacgaaagat gtaaggaaat tcccatggca ttgaccaaga agcaaatcaa
     1381 gcagctgcgc gcgatggcga actcgctgaa gcccctgttc tatgtgggca agaacgattt
     1441 gaccgagtcg gccgtcaatc aggccgatga gaccattgag aagcacgagc tcatcaagtg
     1501 cgccgtgcag gacggttctg gcctgaccgc caaggaagcc gccgccgagc ttgccgagca
     1561 gctgaacgcc gaagtggtgc agtccatcgg caatcgtttc gtgctgttcc gtcgctcccg
     1621 gcgcgacgat gtggagcata tccgcttggt gcgcgagtaa tgttcgcccc gctcgatccc
     1681 gtagcttgat atgagtaatg gcggttggaa tcagtccgat tccaaccgcc attactcata
     1741 ttcgcccagc gagcaccgtc actgatccaa gatggacccg acgattcccg caacaatggt
     1801 caggaccaag gaccccagta ccgaccacca gaaaccgtgt acgaagacgc ccacgccgaa
     1861 cagactgacc gccagccagc tggcgagtcg catgaacagc caattgataa tcaaggtgac
     1921 cagcccgaga gacagaatcg caaacggcag tgcgatcaga tgcacaatcg gcttgattga
     1981 cgcattaatc agcgccatga acaaagcgaa cgcggcgatg ccgagaatcg gaggctcccc
     2041 caccggggtc atgccgggag tcaccgcgac catgacggcg gcggcgatag tcataatcaa
     2101 ccagctggtc aggaaacttc tcatggccca atcgtagcgc ggcaggtttt actattgggc
     2161 aaaacatgag atgatgttgt gcagaaacgg taagaaggta ttgccgttcc cgattgagag
     2221 gggactgacc cgcatgactg ataatcaaca acaaccgtac ggttcggaca gtgttccgca
     2281 gccggcacag cccgcacagc cggcacaacc gtcttacggc caagatgccg cacagcagcc
     2341 gcagggctat ggcgccccac agcccgccgc atacgctcaa cccgccgccg gaagctacgg
     2401 ccagcagcaa ccgcaatacg gccagcccgc cggcgcatac gctcagcctc agtacggcca
     2461 gccgcagtac ggccagcccg cacagcccgc agcgggctac ggccagcagc ccggctatgg
     2521 acaggcttac cagcagccga acgcctacgc gcagccgcaa tacaccgccg ctccgtatgc
     2581 cgccgccccg gtggggcaat cgtccaagtc caagctggct gccggcctgt tgggcatctt
     2641 cctcggcgga cttggtgtgc acaacttcta tctcggcaac accggcaagg ccgtggccca
     2701 gctgctgctg acgcttgtcg gctggattct gctcggactt ggcccaatcg cagccgccat
     2761 ctggggcttt gttgaaggca tcatcattct gtgcagcaag cccggctcac cgtggcataa
     2821 agacgccaac ggctacgaac tatcagactg aaactaacct cgggccacgt tcattgggcc
     2881 cctgatcaac cacgcctctg caggtatcat ctcgatgccg cagtcgcagc gggatgcgac
     2941 acgccggccg tgtacatgag ttcatgtaca cggccgtttt ctttgttgct ttcaccctca
     3001 aaacaaacat ttatcatgtt cacttttgca agatatgaac atcagacatc acagactacc
     3061 ataacttccc aattgcatta ccaatagatt aataaccggc aaaacggctt atatccgcca
     3121 tttttcatgc taggctttga aaccagcaca tcaacaaagt gcacaatcct tttctacaga
     3181 gaaggtaaca tgttgttcaa ttcatatgtg aatcgaacaa tgccagtact tccctaggtg
     3241 cttgcttggg gaacgccatg ctgaaaggaa cacattatgg cggagggaaa agtcatcatc
     3301 gtcaacccgg atatgttcgg caaggacccg gattcgaaaa caaccaaggc caatgaagtg
     3361 gccaagagct tcggcctgag cgacgcggcg ttggccgagg tcgaggactt caaggcacaa
     3421 ctcaccaaac acaacgcctg ggatctgccg ttcatgggat acgtcaacga ggatggctac
     3481 ggttacgcct acgtgcccgg tgccgccgtg gtgtacgacc cgtactggga cgcccaccaa
     3541 gcattcctgg cgctgcctaa ggacgtgcag accgcattcg ccattcgtat gctgttcacg
     3601 caccgtccgg tcgaccgcta tggcgccagc atgttcctgc attaccagcg tggcttcaac
     3661 gtgaagttcg agggtatcgg cgcgaaccag tactgattgg tactgactat caaccatctt
     3721 tttcggattg cccgataagg ggcgtataac aacggtgtta tacgcccctt atctgtattc
     3781 agtgtcaggc gatggccgcc cgccgcccga cacaggcgaa tcagtatcgc acgagattca
     3841 acagttcctg ctgtttggca tagcgggaaa cgttggtcag ggcgatgcgg atgatgtgtt
     3901 cggaagtctt ttccaaatga ttaccgccgg ccacatgcgg ggtgatcagg caatgcggct
     3961 catcccacaa cggactttcc gccggcaacg gctcaggctc ggtgacgtcc aatccggcac
     4021 cgtggagccg tccattgtgc aaggcgtcag ccaaggcctc gggatcgatg gcgtcgccac
     4081 gcccggcatt aatcacgatg gctgtggatt tcagacgacc cagacgatcc gcattcagca
     4141 gatgatgggt ctgcggcgag cgcggcaccg ccatcgccac cacgtctgcc aatggcaaca
     4201 gcgtctccag ccgctcgaag ccatacatct tttcaatgcc cttggccggc acgtccgcat
     4261 gacggcgcac gccgaaagta cgcataccta cggattgggc aagctgagcg aaatgcgacc
     4321 cgatgtcacc gacgccgata atcagcgcga tgccaccttc cgggctcaat accggtcctt
     4381 catcctgcca ctgatgatca cgctgattgg aggcataccg gttgaggttc ttcatcaatg
     4441 cccacatggt ggcgaacatg tgttccgaca cgctttggcc atacgcgccg ctcgcattgg
     4501 tgaccatggt gccgcgaggc agcacacccg ggcagatgta cgcatccaca ccggcgctga
     4561 aggtctgcaa ccattcaaga cgggtgtatt gggaagcctg cgagaccgga aagttgccga
     4621 tgactgcggt agctcgggag cgaagctcct cgggaacagc ggccgaccag accatgtcgc
     4681 cgcggtgcgc ttcgtcgccc acgaattcct gcggcacatc gcgagccgcc ttggcgaaag
     4741 cccgccgttc tccttcgttg agtggcaggc agttgacaat cagcttatgc gtgttctcaa
     4801 tacccatggt tcaccaaact aggcggcagg gcagatatta tcgcggtttc ctcatgctga
     4861 accaatgccg aactgctacc ggaccaatgc cgaatccgcc ccatcaaacc ccaccaaacc
     4921 aagggacaac ccattgcgca tcgaccgtag cgtccgcccc gaaccgtaca ctcacaggtg
     4981 gttttgtgat tccatagcgt gcagccaagg cagtacgatt gccggtttat ccgcacgcac
     5041 aggtacctac gccggttggt aaaccttaca ttccatacct tgcaaccgaa cgtttaggag
     5101 catgtatgcc tgagcagcat attgataacg agaagattcc cacacgtctt atcggcgcga
     5161 ttgtggctgt cggctcactt gccttcattg gcattctgac cgaaaccgtg atgaccgtgc
     5221 ttttccccgc actgatgcgc gaattccatg tcgacacggc cactgtgcaa tggattacca
     5281 cgatttatct gttggcggtg gccgctacca tgcccatcag ctcgtttttg aagcgccgat
     5341 tcgcgctgaa aaccattttt cttgcggctg tgattctggc catcgttggc tcgctgatca
     5401 tgatcgtcgc ccaagccttc ccgctgctga ttgtcgcccg cattattcag ggcattggct
     5461 caggcgtggc cacgccgttg atgatcaaca tcattctgga gcagtctcct cgttcgaaaa
     5521 tcggccgatt gatgggcgta ggatcgctgg tcattaccgt ggctccggcc atcggcccga
     5581 ccgtcggcgg cgcggtgacc acagtgctac cgtggcgcgc gattttcgtc atcgccattc
     5641 cgctggtgct aatcgcagca tgcatcggca tgaagtgcat tgagcagaag acaccgaccg
     5701 aggctgccta tcttgacccg attcagcttg ggtccattgt tgtcgcgctg gtcggtctgg
     5761 tgctggcgct caaccaaggt ggcgtggcca tcagtgcggc ggtgtccggc aagtcagcca
     5821 cacgctccgg cattatcgcc attgtcagtc tgattgtggg cctcgccttg ttgatcgtgt
     5881 tcgccatgag ttccaagcgt gccttctccc cgctgctgcg ccttggcatt ctcaaggatc
     5941 ccgccgtact gttacacgcc tgcgcatatc tgctgctgcc attggtcgcc atcggctacg
     6001 gctatgtcat caccaatgtg gcacagctct cgctcggcac cacggcattt gtggccggct
     6061 ctctggtgct gcccggcgca ctggtcggcg ctgtctgtgc cccgcttggc ggctggttct
     6121 acgataagtt cggcgcggtc aagccgattc tgattccgct cggcatcgct attttgggcc
     6181 cagtgcttat gctggtgttc tcgatgcagc ttacgcccgt gctgctcgcc ggattctatt
     6241 tcattttcgg tctgttctat tccatcggca atgcgaatgt gatgaccagt ggcctgtcgg
     6301 aggtctctcc cgagttcaag ccggacggca acgccatttt caacacgtgt cttcagtttg
     6361 gcggctctgc cggtaccgca ctgttctcca cgattctttc cgtggctcag gccggcgcag
     6421 gagaagaagg caccgctgag tttgctcacg ccactgccgt cggcggcaca tggacgtttg
     6481 caacgatgac ggttatctgc ctgattggct ggtgctgtct ggccaccgcg ttccgcattc
     6541 gcgcggcacg taagcgtgca taaatctgat taccaataga gtcccagctt tccaaacacg
     6601 tgtggggcgt gcccnnnnnn nnnnccccgg gagaatcccg gggcacgccc cacacgctaa
     6661 tgaaaggaag aaaaggagaa agaaagcttt ggcgggcagt gccggctgct gtgaccttgc
     6721 catcacagca gccgggctga tcccgcctat tcagttatgg tgtgtgtcgt attcggtgcg
     6781 gagaccgagc gggcaagccc gctatttttt atctccgccg accgccatca ccgcgcgctg
     6841 aagcacaacg aagacgagga tcatgagacc gataacgatg gtggtccatt cggccggcac
     6901 accgaagtca gaggtgagcg gatcgaggat ggagcggacc agagagccga gcacggagcc
     6961 gagcacgtag ccgaagccac cggtgatgat cgtaccgccg atgaccacgg aggcaacggc
     7021 gtcgagctcc cagccaacac ccacagtgtt cttggcagag ccgatgtttg cggtgtacac
     7081 gatcgaggcc agggcggcga gagtcgcaga ggtcaagtag atgatgtact gcgtgcgctt
     7141 gaccggcaga cccatgagtt ccgcggagga acgggagccg ccgatggcgt agatggtgcg
     7201 tccggtgcgg gtatggtgca ggaagacgta gccgaagacc acaaccacca gtgcgatgat
     7261 cacgccgacg ttgaaggaca gatcgttgga gatcttcgga ttgtcgatga tcttgatcac
     7321 gttggagatg aacgagaagt cattgccctg cgggaaggtc agcgagtcgg tggagatgat
     7381 ggaggcaaga ccacgggcca ggaacatcgt cgacagggtc gcgatgaacg gctgcatgtt
     7441 gaattcctcg atcaaggtgc cggccagcag gccgaacaca gcgccgatga gcagcatgat
     7501 gatcatgacc aggaaggcgg gcacgccggc gttcgccagc ttcaggccga cgactgcggt
     7561 gatggccacg atagcaccga cagacaaatc gataccaccg gtcagaatcg gcagggtcat
     7621 ggccacagcc agaataatca ggtaggcgtg gtcaatgaac agggaggaga tgaagcccag
     7681 tcgaatgtag gtgccgaaca acgcctgacc catgatgatc atcaggatga agatcaccac
     7741 ggccgcgagg gtcgggatca tctgacgatc gagcttgaag cccttcttgg gagccttcac
     7801 tttgtttgcc gtagctgttg tcatgctgcc accgccttag cttgagcctt gagcgcgcgc
     7861 ttgcgtttca tattcgcgct caggttgtga atcttcggag cctgcatcac gcagatcaca
     7921 atcacaacga cggcgaagaa ggccggagtt gcctcggcgt tgacgcccag ggtgatgatg
     7981 gtcttgcgga tcatggcgat aattacagca ccgacagcag agccggcgag ggagaactta
     8041 ccacccagca gtgaagtacc gccgatgacg actgccagaa tggcgtacat ttcgaggtcc
     8101 tgaccggtct taaccacgtc gacacgcatc acggatgcgg tggcgaacag accagcgatg
     8161 gccgcgagga agccggaaat cgcgtagacg aggaagagga tcttcttcgg cttgataccg
     8221 gtcatacggg aggcttcctg gttgatgccc acggcctcaa tcatcatgcc catagcggtc
     8281 ttgcggcaca gcaggccaac gagaatcaca atgataacgg cgatgacgaa gttggcggga
     8341 atgcccagaa tgaagccgtt ggcgaaccac ttcagcggtt cgttgccagc aactgcggag
     8401 gcgtcggtgt tctcaccgga ggtgatgacc ttggccatac cacggccggc gagcatcata
     8461 atcagggtgg tgatgaacgg ctgtaggccc aggaaggaaa ccagagcgcc gttgacgcag
     8521 ccaatggcca gaccaacagc caaggcgatg aggatggaga gccacacgtt catgccattg
     8581 gacagggtct gcatggcggc ggcacctgcc actgccataa cggaaccgac cgaaaggtcg
     8641 ataccggcgg tggagataac caaggtcata ccggttgcaa tcatcaggta tcgggcagat
     8701 tcctggagca tggtgatcag cgggccggcc agaccaccgg tgttgctgtt ccagctcaaa
     8761 gccaggaagt catgctggaa gatggtgcag atgatgacca gaagaatgaa tgcgacgatc
     8821 gaccaggtca ggttgctgct cagcagcttc ttgacaaagt tgttgccctc ggcttttgcc
     8881 ttttcagcca tctcatgcct cctttccggt gtttacgttg gtgttagcga tggtttcgac
     8941 gatggtcgcc tgagagacgg tgtcgtcgtt ttcgatttct gcgatcttgt ggcggtcctt
     9001 gagaacctcg atgtcgtcgg acagacgcac gacctcttca agctcggagg agatgaagac
     9061 aacgcccatg ccctgagaag ccaggtcaag cacgacctgc tgaatttcag ccttggcacc
     9121 gatatcgata ccacgggtcg gctcgtccag aatcagcagc tcggggtgcg tggccagcca
     9181 acggccaatg agcaccttct gctggttgcc gccggagaga ttcttgatcg ggcgatccgg
     9241 gtcggcggga cgaacgttga gttccttcat gtacttgtca acgatggcgt ccgcttcctt
     9301 cttgggaatc ggcttgaaca taccgcgcgt tgcctgcagg gcgataagga tgttctggcg
     9361 gacggtcagg tcgccgatga tgccctcatc acgacggttt tcggtggagt acgcgatttt
     9421 gttcttcaaa gccgtgtacg gatcggagat gttgaccttc ttgccgttga gcgtgtaggt
     9481 acccgaatcc ggcttgtcgg caccatacag gagtcgacca agctcggtac gaccggagcc
     9541 cagcaggcca gcgaagccaa cgacctcacc cttgtagatg tcaacatcaa ccgggttgat
     9601 ggtgcccttc ttgccgaggc ccttgacatc gacgatcggc ttctcgccag gggtgatttc
     9661 acggcgagcc ttcttggcac caatctggga cagctcggcg gcggacttac caatcatcat
     9721 gccgatgagt tcgtcgcgcg gggtgtcctt ggtcatgacc tccttgatga actggccgtt
     9781 acgcagaatg gtcagacgat cggtaatctc ataaatctga tcgaggaagt gggagacgaa
     9841 gaggatggcc acgcccgagt cacgcacctt gcgcatgatc gcgaacaggt ccctgacctc
     9901 gttggcatcc agcgaagagg tcggctcatc gagaatcagc accttggcgt tgataaccat
     9961 agcgcgggcg atggcgacca gctgctgcat agcgatggag atggagctca gcggagtgtg
    10021 cgggtcaatg gattcgaggc ccatctgtgc caaatacttc ttggcggcct cgtgggtctt
    10081 cttccagtcg atgccgaagg ggccgcgctt ttcgtggccc agcatcacgt tctcaccgac
    10141 ggaaaggttg gtgcacaggt tcacttcctg atacacggtg gcgataccgg cgttttgtgc
    10201 gtcgagggtg ccgttgaact gctgaggctt gccgtccacc ataatggagc cggcgttaat
    10261 cttgtacaca ccggtcagag ccttaatcat ggtggacttg cctgcaccgt tctcacccat
    10321 cagggcgtga acttcacccg ggtagagagt caaatcaaca ccatccaagg ccttgacgcc
    10381 cgggaattca atcgtaatgc ctttcattac gacgatgggg tttttatctg tcatgtcttt
    10441 gccttaaacg agaatgagat gttatcgttt tgtgatattt gagccacaca tgagtgatgg
    10501 cgtggaagga acatccttct cacaccatca catcaattat ttggtccagt tatcaatgag
    10561 tcgcagcaga cttatcagta ggcgcgggtg ttgttgtcca gggcttcctt ggcggaggcg
    10621 gcgtcgaagg tcttggactc gatctcgatg tccttctcaa cggtcttgcc atccagatag
    10681 tccttgacgg cctgagcggt ttccttaccg aagatcgggt tgtactcgat cacgtaggag
    10741 aggtcgccat caacaagagc ctgcagagcg ttcttggtac cgtcgatggt gatgatcttg
    10801 accttgccct tgaggccggc ggcgtcaaca gcctgagcgg caccgaggcc catctcgtcg
    10861 ttctgagcga agatgaactg cgggttgtcg gacttgtact tgtcgagcag accagcggtc
    10921 acggtcttgg cctcatcagt ggaccagtta gcggactggg actcaagaac cttgacgttg
    10981 gaggcaacct tgttgtccca accagtgcca cgatccttca ccacggacag gccggcaggg
    11041 ccttcgagga tgaagccgtt ggcgccatcc gggaagttct tgttcacgaa ctcggcagcc
    11101 tgctcgccgc accagacgtt ggacggtccg atgtgagcaa cgatggcctt cttggcctcg
    11161 gcgtccttga cgtccacgtt acggtcaacg gtgaagaccg gaatctcagc ctcagcggcc
    11221 ttcttcaggg agtcatccca accggaatcc tcggtggagg acaggatgat ggcgtcgact
    11281 tcgtcgttaa cgaacttgtt gaacgcctga atctgcttct gctgatcgtt gttctgggtc
    11341 ggagagtagg tcaggtcaaa gccggcatcc tcgaatgcct tctgaatgtc cttctcgttg
    11401 gcggtacgga agccgccctc aggaccgaca gccacgaagc caaccgtctt cttgccggag
    11461 tccgagctgc cacctgcgtt gctgggaccg catgcggcaa cgctgacaag cgcagcagca
    11521 gaagcaacga gggcaatggc cttcttccaa ttcttcataa atactcctcc tcgagcagtg
    11581 tttatttttt ggatgatctt tcatcctgtt tggtgactgt tcgtcatcga cagcctctag
    11641 tatgacgctc acaggccaaa tgttcaatcg catgcgataa cagtttgata acaggtttat
    11701 aacaaattaa taacagtggt tcaaaaatgg cgtcgcaaca ctgttttgcc ttgataacag
    11761 accaataata gtgattggtt gcgcagcggc aatgccgctc gccagccgag cgacaaagcc
    11821 gttgcctcat acaacaagaa aggctattat gaacaccgtt tttcttatac catctactgc
    11881 gcaccgaggg cagtgacctg gctatcggcg atggtccagg aaccgtcgag tgagccagaa
    11941 acggagcgaa caatacccaa atcgtccaca atggccaggc gacgggacag gctaatctgc
    12001 cccgatgcca tcgacgttat ggttccatta ggcagacgct gcgcattggc cgggcctggt
    12061 gccatttgac gccatgcctc ttgttgttcg ctatttccct caggaggtgt ggtggcgtac
    12121 accagattca gatcgttgta gaacgtgagc atggtgacgt gtctgagcac actcacttgg
    12181 gtggccgctt tgctcagacc gctcagtgtt ttatcaccgt tgcgcgccac accggtcatc
    12241 atcacgccgt tcgtgtcctc gccctcgact gccaacgcca atcggcaccc ttccggagaa
    12301 accgccaatg ccacaatgct gtcggcagcg ccaagccaat caagcttcag atcggtttcc
    12361 ttgcccccat ctgacacatg caattcgcgc ccgttctcgg atacagccca tacttcgcca
    12421 tccagacctt cggtaatcga tcgcatcggc tcgccggaga acatcacccc acaggacgca
    12481 ccatcagatt tcaggcattc gaccacgccg tccgcacgca acaccgcgcc gccggatgag
    12541 gaaaaaacga agccccgagc atcgtcatat ccgggggcct cccctacacg caacggactg
    12601 gagctggcca gggaaacgat atgaccaccg gtcaacgtgt atacgcccgc tgtcggctgc
    12661 tcggtagtaa gtttcacatt tgcgtcggca tcggaatagt ccactccatc gccggtaatc
    12721 ctcagcgcgt attcggcatt gccgtcgccc atagtcagac ggatgcgatg taccagcagg
    12781 cctcgttctt cctcgttcaa cgcattgata ccgctgttga ggtgtatctc caccacattg
    12841 ttcttcaccg gcacgctgtc gaccgcgagt ttgatggtag acaagccggc gcctcgcagc
    12901 actccttcca accaggaggg tgcatccgag agtacttcac cgactgcctg ggtgcgccag
    12961 ttgcgccagc tcagccatcg aacatccggt atgagctgtt tgccggatgt gctcacctga
    13021 tacacggata cttggcggaa gacttgttca aaatcggcgg tggatatcac cacgccgttc
    13081 tccaacgagg agatgcgcca ctggccgctt cttttaatca agatataagg aactttgctg
    13141 atcgtgctgc tatctgtcgg cgtatacacg ccatgcgagt ccagagagcc gaccacttgg
    13201 agctgtactt ctacaatcag cgagctctcc gccccttgcg gggtactcat cgtattggca
    13261 cggcggcgga aatccggcgt accgctgtag accagtgccg aggaatcgcc attccatccg
    13321 gccgacgccg aaccggtcag aaactcacgg gccacacggt agccatcgga ctgtacgccg
    13381 gcaggcatgg catcatagaa ccctttgacg atggtttccg gctgcgcatc gtccgcaggt
    13441 ccctgaggat tggtgtaaac acgctgggtc tgctgttcca ccggtgccag agtctgcacc
    13501 gagccgctaa tcggcaaacc gaacggactt gagcatgccg tcatcgtgac gcaacagact
    13561 atggccgcgc ctgcggcggc aatcgttctg gtcactcgtc tcatagcggc ctccccatca
    13621 tcgtgtcgcg gcgaacctca tgataatcat gtgtgacttg gctggtggcc acaccaaacc
    13681 caccggtaac acgcaggtca tccggcgttt cagaagcaaa attcaccggc agttcagcgt
    13741 ccgccacctc accttggtcg gggtcacgcg gcagcaatac caagaaccag gtgccctccc
    13801 ccactgccga acgtacacgg atactaccgt ggtgcagcaa ggcatcagtc atggcgatgg
    13861 acaggcccaa tccggtgccg ccggtaacgc gtgaacgtga aaggtcgcct ctccagaatc
    13921 gatcgaatac atgagcgact ttgtcttcgt cgataccaac gccatagtcg cgaacgctga
    13981 tggccacggc tttgcggttg gccgcgacgc gcacttcgat cggccggtcc tcagcgaaat
    14041 cgacggcatt ggccaacaga tttctgacga tgcgaatcac tcgacgcgaa tcgatacgag
    14101 tcaaaacctg cacattgggc agatacgtgt gaatcggcac ccgtttggct tgggcgatgc
    14161 cgtcaacttg gtccactgcc gtttcaattg gttcgcataa atcggtttc
//
LOCUS       DAWBMW010000022        14077 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_1106_length_14077_cov_3.290543, whole genome shotgun sequence.
ACCESSION   DAWBMW010000022 DAWBMW010000000
VERSION     DAWBMW010000022.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 14077)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 14077)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14077
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1106_length_14077_cov_3.290543"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    13777..13786
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2658 a         4375 c         4332 g         2702 t
ORIGIN      
        1 gagtagatgt ccgggccgta cttggccagc aacgccttgt tggcggtgac cacggaagca
       61 ccggattcga tggcggcaag cacgaacttg cgggccacgg tggtgccgcc gatgagctcg
      121 atgacgatgt ccgagttggt ggccacgctc aaagtatcgg tggtgacgat ggactggtcg
      181 atccacggga acttctcagt ttccttcggg tcgaggcagg cgacgccggt cagttcgacc
      241 gggcggccga tacgagcgga cagttcgtcc ttctgctcga caatcaggcg ggcggtctgg
      301 gagccgacgg tgccggcacc aagcaggcct acgcgaatcg gggtttcgtt gttgcgagcc
      361 aattcagggt ccttccaaaa agttttctat atcccaccgc tattttacgg attatttccg
      421 acagcagacc gaaagtactc atcaaagctg acggcccttt tcgtattatg acggatttgg
      481 ccgcacatta cagactgtca ggcgctcgcc tctgcacgcc gatcactcgc tgacgtcgag
      541 gttgagtagg tcgtctacgg tctggcggcg gagcatgacg tgcgcgccgg actcgctgac
      601 agcaaccacc gccggaatca gcgcttggtt gtagttgctg gccatggtgc ggccgtatgc
      661 gccggtaacc ggcacggcga gaatgtcacc gcgcttcaaa tcggcgggca actgcacctc
      721 gtgaaccacg atgtcgccgg attcgcagtg cataccgcac acacgggcaa gcatcgtttc
      781 agcggaaccg gtgcgattcg ccagacgcgc ggtgtagtcg gcaccgtaga gtgccgggcg
      841 aatgttgtcg gacatgccgc catccacaga cacatacacg cgctcggcaa tcgggttgcc
      901 gtcactgtct ttggcatcgg acaagtgcac gtgcttgatg gtgcccacgc ggtacagggt
      961 tatgccggcg ggcgccacga tgtagcggcc gggctcgaag ctgatggccg gggcgggcat
     1021 gccgagcgca cggttggtgc tgttgaccgc atccgccaag cgagtgagtt ccacgtcaat
     1081 gtccattgaa tcttcgccat cggtgtaggc cacggagtag ccgccgccga ggtcgatttc
     1141 gggcaggatg taggcgtcgg tggcccacag ggtcttgcgc agcagcatca tgcgcttggc
     1201 cgcgtgaatg aaggcgtccg catcgtggat gttggagccg atgtgcgagt gggctccgac
     1261 cagttcgagt acgtcctggt tggcgaggat ggtcttgagc acggcgatgg acggaccgtc
     1321 cgcaatggct tccatcgcgg cggccagcgc cttgtctgtg tcggagactt cctcgtggct
     1381 caggtcgtac gggtacttga cgtcgtagtg cagctcgcgc tctgatttct cggtcttttc
     1441 cgcgccattt tccggggaat gcgtcgcaga gatacgcgcg ttggtagcgg caggcgtcac
     1501 gtctttgagg tcgttcagca cgtcgagcac agcggcgtcg gccccggcgg ccagcagcgg
     1561 cacgccgaac ttctggtcct cgtgagcagt ggagatgtac tcgtggccgc cggcgtggac
     1621 accgacggtg acgcgcagca tcacacgggc gcgtttgccg agcttgcggg cgatggcagc
     1681 aacgcgctcc ggctcgtcgg gctcgtcgat cacgattttg gcgaagccct gttcgatggc
     1741 cagcgcgatt tcttcatcgg acttgttgtt gccgtgcagc accagacgac gtccgggaac
     1801 gccagcggct agggcgattt tcatctcgcc catcgtgcaa gtgtccacaa acatgccggc
     1861 ctcggtgacc aatcgcacga cttctttgga caagaacgcc ttgccggcaa agctcacgtg
     1921 cgtagtggag gtgttgaatg cttcggcggc agcgcgcacg aagtgcctag cacgtgcgac
     1981 tacttcatca gtgtcgatga gatagagcgg cgagccgaac tcgtcaagca gcgattcggc
     2041 ggtacgacca tgaaattcga tggagccttc ggcattcaca gccgtagcgg ccggccaaat
     2101 tggagaaata ggcataatca catcttttcg ggggcggtga cgccgaggag gtcgaggccg
     2161 ttggcgatga cctgctggac ggcgtcattg agcttcaggc gggcggcggc acgggccggc
     2221 tccgggttct tggcaatctc caaggccttg cgagcctcgt cgtcgccacg ggtttccgga
     2281 tcggtcaggg ccatcggcac cacgcgctct acgttgtacc acttgtgata ggtggcggcc
     2341 agttcttcga ggtaacgggc caccttgtgc ggctggcggt cgtcggccgc agtggcgagc
     2401 acgctcggga attgggcgag ggcggcgagc acttcgccgt cggcctcggt gtcgagcagg
     2461 gccaggtcgg caccctcgta gctgatgccg gcagcggctg cgttgcggtc cacgttcttg
     2521 gagcgggcgt gcgcgtactg cacgtagtac accgggttgt cgttggtgtg cgaggcgagc
     2581 agggccaagt cgatgtcgaa gttctggttg taatcggagc gggccagcga gtagcgggcg
     2641 gcgtccacgc cgaccacgga gaccaggtcg tcgatggtca cgacgttgcc ggcgcgcttg
     2701 gacatgcgca cgggcttgcc gtccttcatc acgttgacca gctggccgat gagaatctgc
     2761 atgttcttgc cgggctcatc gccgaaggcc gcgcacatgg ccatcatgcg gccgatgtag
     2821 ccgtggtggt ccgcgccaag catgtagatg gccacgtcgg ccgagttttc ggcgcgatgg
     2881 cgcttgtccc agtagtaggc gatgtcggcg gcgaagtagg cgaactcacc attggacttg
     2941 atgatcacac ggtccttgtc gtcgccgtgc ttggtggatt caaaccaagt ggcgccgtcc
     3001 ttgtcgaaga tgtcgccgcg ggacttgagc tcttcgatgg cggcgtcgac cttgccgtcg
     3061 gcgtacaggc tgttttcatg gaaccagacg tcgaagttca cgcggaagtc cttcatggac
     3121 ttctgaatct cgtcgaacat catcggtacg gcgcgcttgc ggaattcctc gcgcacttcg
     3181 gtatcggcct cgccgagcgg ttcaccgtca tcgttcaggc cctgatcctg gtgggccagt
     3241 gcggtcaggt ccacgccgtc ggactcggct tcggcctgaa cacgggcggc gatctcgttg
     3301 atgtaggcac ccttgtagcc gtcgcacggg gtttcggtct ggtagccggc ctcacccaag
     3361 ttattggcct cggcccaagc ggcgaccagc gacttggcga agcggttgat ctgctcgccg
     3421 tggtcgttga agtagtattc gcgcaccacc ttggcgccgt tggcctcgag cacgcgggcc
     3481 atggcatcgc cgacggcagc ccaacgggtg ccgccgatgt gaatcggacc ggtggggttg
     3541 gcggagacga actcgagatt cagggtctgg ccgcccagat ggtcgttgcg gccgtattcg
     3601 ttgaccttgt tgagatgctt gtcggcgtcg gtcacggcac cggcggcgag tacggtgtcc
     3661 accacggcgg cggccgaggc ggagtcaagc gtgatgttga tgaagccggg gcctgccacc
     3721 tcgaccttgg caatgccgtc ggcttcagcg agcgcggcgg cgaacggctc ggccagatcg
     3781 cgcggcttca tgccggcctt cttggccagc tgcatggcga tgttggaggc ccagtcgcca
     3841 tgcgcacggt ccttcgggcg catcacggcg agcttgtcga cgggcggaat cagctcgtcg
     3901 gtcaacgcgc cggcctggcc ggcggcgacg aggttgtggg caatgctgga aatcagttca
     3961 cttaaggctt caggactcat tcttctaagg atactgagaa cggcgacact tgcgctctgg
     4021 tatgcctata tgctgcccct gctggcgggg gcaagcacac agccgaaaga taccaagcga
     4081 aaaagggtag gcgacaatcg ccggaatcgt tggaggctcg ccgaaaccgc tatacatacc
     4141 gtcggtcggt atctatagtg atctgcaaca tcaagcacag tagggaggcc accatggcac
     4201 gtaacactca ccccgaggtg acgcgcaccc gcatcctcga cgccgcacaa cggctgttca
     4261 tggcccaggg ctatgagcac acctccatcc agaacatcgt ggacgagctc ggcgacctct
     4321 ccaaaggcgc gatctaccat cacttcaaat ccaaggaagc catcctcgaa gagctcatca
     4381 accgagacaa caatgtgcag gacgacttca acgaatccgt catgaatcgc actgacctta
     4441 ccgccctcga aaagttccgc gtgctatggc ggcattcgat gaccgagcaa gaccacgtgc
     4501 agatcatgcg agccgccatg ccgatgctga gcgatccggt ttcgttcgcc gcgaatatgc
     4561 aggtatggag caagcatctg cccgaacgct tcctgccgct gatcgaggcg ggcgtcaagg
     4621 atggctccat ccccaccgag tatccgcgcg aggccgccga gctgctttcg ttgctgccga
     4681 attattggct gatgccgtat ttctacccgg tgaccctgcc cgaaatggag catcgcatcc
     4741 gctgcttggc caccatgctc gacgcgatcg gcgtgccgat tttcgatgac gagctcatca
     4801 cggtcgccgc gaagggcatg atgacgttcg caggggagcc ggagaaaggc tcagaatgaa
     4861 tagcacagtg aaacccgaga atgacatcgc cggcaccgcc acccaacgcc cgctgctgtc
     4921 ccgcgacttc gtgctgctgg tcgccggaca aggcatctcg ctgttcggca atatgatgct
     4981 gcgattcgcg atgtcgatgt gggtgctgga caaaaccggc tcagccacca tcttcgcctc
     5041 ggtgctggcc atctccatcg tgccgaccat cctgctctcc ccgttcggag gcgtgctggc
     5101 cgatcgcgtc aaccgccgca ccatcatggt ggcgctcgac gccatctccg ccgtgctcgt
     5161 gctggcgagc gccatcgtct tcgccaccac gggcttccat atcgtagcca tcgccaccat
     5221 gcaggtgctg ctcgccgtac tcggcgcctt cgaaaccccg accgtgcagg ccgcactccc
     5281 ccagatgttc cggcagtacg gcccggccac catgcgccaa ggcatggccg tgatcaatca
     5341 ggtccaacaa ctaagcagtc tgctgccgag cttcctcggc ggcgtgctgt atgcgatgtt
     5401 cggcatccgt ctcatgatga tcatcgccat cgcaagcttc gccggcgcag ccgcgctcga
     5461 atgcttcatc cgcctcagtg ccccggatcg cggcgacgag gagctgccca ccccacttga
     5521 agacctcaag gcaggcgtgc gattcctcat caaggaccgg ccgaacgtgt tcagactgct
     5581 gttgttcgcg gcggcgctca actttgtgct tatcggctat tccggcgtcg gcttcccgta
     5641 cacgattcgt acagtgctcg gcttcgacgc cactgtctac ggcatcgccg acggcctgat
     5701 cggtgtttcc ggcgtggcgg gcgcattcat cgccggcctg ttcgccgcca gactcacgat
     5761 gcactggatg ccggggctga tggcgacgct tacgctggcc atggtcccac agggtattgt
     5821 gttcctgctg ccggtcgacg catggaccaa actcgtggtg ctgatcgtat tcacctgcgg
     5881 cacgatggtc gccagttgct tcacgaatct catcgccgtg cccgccatcc agctcagtac
     5941 gcccgaggca atggccggca aagtgatgtc catggccgcg gccgtgagca tgtgcgccca
     6001 gccgctcggc cagatggtgt acggctgggc ctacgaccaa atgccggtcg ccatcgtgct
     6061 gttcatcacc accgccctgt tcgccgtgct caccgtgctg atggttccgc tcgccaagca
     6121 gttcgaggac tgacgatcgg cgcgcacgcc acatcctgcc gcccggccat aattgaatca
     6181 ccgcacgaaa aggcacgaaa aggcacgaaa gaggtaccta tggccacgct caacccccaa
     6241 tcattggtca cgttgtggca aatcgaacag tatggttcgt tctccgccac ggccaaggcc
     6301 acgggttggt cgcagccggc catcagccag cagatcaaaa agctggaggc acagtgcggt
     6361 tcgactctgg tgcaacgcac ctcgcatggc gtggaactca ccgccaccgg ttcgatgctc
     6421 gcccgccacg gcgaggccat cgccgatcgg ctggagcggg cggcccgtga agtcgaggat
     6481 tatcggcatc atcggtttga tcacctgcat ttggtggccc caccgtcaat ctgctctact
     6541 atcgcggccc gtaccgtggt caagctcagt atgttcacgg acatcgagct gagcctgatt
     6601 cagatggagc cgccggaggc gatcggtctg atttcccaag gcaaggccga taccgccgcc
     6661 gtgttccgct acagcagcat cccgaacttc ctgcacatcg gcgacgacct gaccttccat
     6721 tcgctgggtt acgatccgat gcgactgctg gtgcgccgct ccagcggcat cgccaaacga
     6781 ttcgaggaga ccggcgaacc tgtgccgctg tccgccgcca aagacgagca ttggatcgcc
     6841 ggctgcccca cctgccgcgc aaatttggtg aaactggcca cccgtgccgg attcaagccg
     6901 gatatccgcc actgcacgga tgattactgg gccacgcaga acctggtcga ggttggtatg
     6961 ggcgtctcgc tggtgccggc gctcgatacg catatcaact tgcagggcga tctggtggcc
     7021 tgtcccattg ccgacgattt tgcggcgcgc gaggtcggca tcgtgactcg tgccggcgac
     7081 catcgccccg cgcttggttc attgctcgag gagctcgagc gcacgtcact caaatatttg
     7141 agtgccaagt cgtcgcacag cgataagcaa gcttgcgagc gccatcacga tttcggggag
     7201 ccagaccgtt gagcagccct tctgcaccgg gaacaatgac atgttatgag catgaacaac
     7261 attccccagt caacgacttt gagcaacaca accgccggca cctcttgctt tgatgccaat
     7321 cacatcgacg tgacgaccat cgaggatctg gagcaggtcg gttcggataa atggacccgt
     7381 taccccggct gcatcggcgc attcatcgcc gagatggatt acggtctggc accgtgcgtg
     7441 gccgaagcca tcgaagaggc tactgaacgt ggcgcgctcg gctacattcc cgacccgtgg
     7501 aagaaggagg tcgcccgctc gtgcgccgca tggcagcgcc gctacggctg ggaggtggat
     7561 ccaacgtgca tccgcccggt gccggacgtg ctggaggcgt tcgaagtgtt cctgcgcgag
     7621 atcgtgcgcg ccggtaactc catcgtggta ccgactccgg cctatatgcc gttcctaagt
     7681 gtgccgcgtc tgtatggcgt ggaggtcctt gagattccga tgctgtgcgc gggcgccggc
     7741 gagagcagcg ggcgcaatga tgaatggctg ttcgatttcg acgccattga gcaggcgttc
     7801 gcgaacggct gccatgcctt cgtgctgtgc aacccgcaca acccgatcgg caaggtattg
     7861 acgcgcgaag aaatgctgcg attgtccgat ctggccgcca agtacgacgt gcgtatattc
     7921 tccgatgaga ttcacgcgcc gttcgtctac caaggccaca cgcatgtgcc attcgcctca
     7981 atcaaccggc agacggccat gcaggctttc acctccactt cagcctcgaa gtcgttcaac
     8041 attcccggca ccaagtgcgc gcaggtgatt ctcaccaatc cggacgatct ggaactatgg
     8101 atgaggaacg cggaatggtc cgagcaccag acggccacca tcggtgccat agccaccact
     8161 gcggcctatg acggcggcgc ggcatggttc gagggcgtga tggcatatat cgagcgcaat
     8221 atcgcgctgg tcaacgagca gatgcgcacg agattcgcca aggtgcgcta tgtggagccg
     8281 cagggcacgt atatcgcgtg gctggatttc tcgccactgg gcatcggcga cccggccaac
     8341 tatttcttta agaaggccaa cgtggcgttg acagacggcc gtgaatgcgg cgaggtcggg
     8401 cgcggttgcg tgcgtatgaa cttcgccatg ccctacccgc tactggagga atgcttcgac
     8461 cgcatggccg ccgcacttga ggcggacggg ttgttgtaga acagaattgc ggctcccctc
     8521 aaagagggga gccgaggaac ggccgaatta gtcgaagtcg gcaccgaggg cttcgagttt
     8581 gccgcggaag tcggcatagc ctcggtcgat caggctgatg ccgtgcacgt ccgaggggcc
     8641 tgacgcggcc aaggccgcga tcagatggct gaacccacca cgcaggtccg gcacgtcaat
     8701 gtcgcgtccg gtcagcgggg tcggtccgaa gatcacggcg gaatgcttgt agttgcgctg
     8761 ccggaaacgg cacggcagcg agcccaggca ctcgcggtac aactggatgg tggcacccat
     8821 ctcgaccagc ggcttggtga agccgaaacg gttctcgtac acggtctcgt gcacgatcga
     8881 caggccgttg gcctgggtca gggccaccac gagcggctgc tgccagtcgg tcatgaaccc
     8941 gggatgcacg tccgtctcga tcgccacggg cttcaagtcc ccgcccggat gccagaaacg
     9001 aatgcccttg tccgtcacct cgaatttgcc gcccaccttg cggaacacgt tgaggaacgt
     9061 gatcatctcc ggctgggtgg cacccttgac gaaaatgtca ccgtgcgtgg cgagcgcggc
     9121 ggacgcccag gacgccgcct cgatgcgatc cgtcagcgac gtgtgcgtgt acccctgcag
     9181 ttccttgaca ccctcgatgc ggaaggtgcg atcaacgtcc acagaaatga ttgcacccat
     9241 cttctgcagc acgcacacca gatccatgat ctccggctca gtggccgcgc ccgacagttc
     9301 ggtcttgccc tcggccagca cggccgccag taaagtctgc tcggtggcgc ccaccgacgg
     9361 gtagggcagg tggatcttcg cgccgtgcag gccgttgggc gcggtgatgt ggatgccgtc
     9421 cttgtgttcc ttgtccaccg tggcgcccag cttgcgcagc gtctccagat ggaagtcaat
     9481 cggacgtccg ccaatcgcgc agccgcccaa cgcgggaatg aacgcttcac ccaaacggtg
     9541 cacgagcggg ccggaaaaca ggatcgggat gcgcgacgaa ccggacagtg tatccacgtc
     9601 ggccacatcg gccagctgca cacgcgaggc gtcgatggtc acaatgccgt tcgcaccgtc
     9661 cacatccacg tccacgccgt gcagacgtag caaatcggac accacatgca cgtcacgaat
     9721 ctccggcacg ttcttcagca ccgacttgcc cggtgccagc aacgcggcga ccatcgcctt
     9781 gctcacgaag ttcttcgcgc cacgcacctt gatcgtgcca ttcaaaggct tgccgccctc
     9841 gacatgcaac acgtcatccg gattctcagc cacgccactc tcctcaatca atcaagattc
     9901 aaaaaatgct accgtccagt ttgccataag ccatgtgccg actccgtgaa atgcacccca
     9961 gccgaacttt gatcaccgtg cacaacggat cccatacccc cgtcgaatca aaccgataac
    10021 gtgatcttcc tcacatttgc cgccacggcg ttctccatac ttctacgcta cgattcaggg
    10081 caatggcatc agacgccatg tccacaagca aaggagcatt catgaccaca gtcgccgtca
    10141 tcggctgcac gcacgccggc acgttcgcca ccacctcgat tctcgccgag caccccgatt
    10201 ggaccgtcca tgttttcgag cgcaacggta cgctgtcctt cctctcctgc ggcatcgccc
    10261 tgtgggtggg cgaccacgta tccgacccga agaaaatgtt ctactcctcc cctgccgctc
    10321 tggccgaagc cggcgcgacc atgcatatgc gcaccgacgt gacgtccgtg gacctcgacg
    10381 ccagaaccct cacctaccgc gcgctggacg aggccggaga ccccgccgaa cagaccctcg
    10441 ctttcgacaa actggtcgtg accaccggct cgcgcccggt catcccgccg atccccggca
    10501 tcgacagccc gcatgtgctg ctgtgcaaga actgggatca cgccatcgcc atcaaggaaa
    10561 aggcgaaaac cgccaagtcg gcagtggtga tcgggtcggg ctatatcggc gcggaaatcg
    10621 ccgagcagtt cagcgtcacc ggcgtcaaga ccacgctggt cgacggcctt gatcgcccgc
    10681 tggccaataa tttcgataag accatcaccg atcaggtggc ggccgcgttc gaggagcacg
    10741 gcgtcacgct cgcgctcggc cagaaggtgg tcgagttccg cgacaatgac gacgacaccg
    10801 tcactgtggt caccgaaaag ggcgagtaca cggccgaaat ggcgattctg gccgtcggct
    10861 tcctgccgaa caccgacctg ctcaagggca aggtggacat gctgccgaac ggcgcgatcg
    10921 tggtcgacga ctacatgcag gcgtccgcgc cgggcgtgta cgccgccggc gactccgcca
    10981 ccgtgttcta caaccccacc ggccagcacg attacattcc gctggccacc aacgcggtgc
    11041 gccagggcct gctggtgggc cgcaacatcg agacgccgac cgtgaagtac atgggcacgc
    11101 aggccacgtc cgccgtgcag ctgtacgatc tctcgctcgc cgcctccggc ctgacccgcg
    11161 ccggtgccga acgccgcggg ctcacggtcc gcgagacctc gctcaccgag gattatcgcc
    11221 cggacttcat gctcaccacc accccagtca cgtcgattct gacctgggac ccggaaaccc
    11281 gcaaggtcaa gggcggccag ttctgttcca aggccgatat ctccggcgcg gcaaacgtga
    11341 tttccatggc gattcaggcc ggcttcacca tcgaccagct cgccaacgtg gacctgttgt
    11401 tccagccgaa cttcgacaag ccggtctact acgtcggcgc cgtggccatg aaggcggcgg
    11461 ccgagtaaga cggctgtacc caccgcaaaa agaaaccccg gcgtgatggg cgccggggag
    11521 agaaaggaga gaagaagaag aaagaggtta agttatgcag cttgttcggc cgtgattaca
    11581 tataacaaca ccagtttcaa tgcaacgtgg gcactggctg ggaattgtct gggaatcacg
    11641 gcccgtacag gctgtttctt gttttggagc cgatttaccg gctcgccttg atcttgtggg
    11701 ccagctcggc gtcccacatc gcgccggttg cggacatcac cgaatccgcg ccggcgtttc
    11761 tgtatgcctg atagtcggcc ggcgaaacca cgccgcccac gccattgatc ttgaaatcaa
    11821 gaccgagctt ctcgcggatc gcggccagac gggcgaccat gtcgaggccg gcaccacgaa
    11881 tcgcattgcc acacacgccg gagcgatcac ggcctgcacc gggcagggcc tgattgccat
    11941 tggcatcgac cagtttggcg gaaatcgtat tgatggtgct gaatccctgc acagtgccgt
    12001 ggcccacggt ggaacgcacc atgagttcaa ggtcgccgtc gctggggatg taggcgagct
    12061 tgaccatcag cgggatgtcg ccgatctcct gcttcacggc ctcggtgatg cggcccacga
    12121 gcagcggatt gtggcatagc aggcggttgt ggccctcgtt ggggcagctg gtgttcatca
    12181 ccatgagctt ggcaccggtt tctttgacca ggcgggcggt ggtggcatgg tcggcgatgt
    12241 attcctcctc gctcatgccc tccacacgcg agccttggaa gctcgggacc agcacttgac
    12301 cggggccggc ggcctcgatg gcggcgcgca tgtccggctg ccaggcatcc gggctctgcg
    12361 agggcacacc gaagctgttg gagatggaaa tcggctgttc atagttggtg tcggccagca
    12421 cgccttcgtc cagttcggcg ctgcccggcg tcagtgaacc gtccgccgac ttggggtgta
    12481 cggcgagcac gttcgggaac gggttgcagc cccaggcgcg cgaacgcacg gttttgtagg
    12541 tcgccagatc gaagcccatg cggaaggcgg ccgtggtgaa acggctgttg agcagcgggc
    12601 ctgcgggaat gccgaaaggc agattgaccg gctggcccaa aaaggtggcc aaggcaccct
    12661 cggaggcacc ctcgctggtg gttccggcgg catcggcacc gttgccatcc ttcagcgcct
    12721 cggcgaacgc gccgaacggc ccttgctcat agttgtcttc gtaactgcgg ctgacatcgt
    12781 aaaacggctc aaaatcactc atgcttcaac gataagccaa cctgcatgcg cacacgcaca
    12841 ctcaacccat catcgcgccg acaaacgaaa taatggcgaa agcggcattg gctaccacaa
    12901 agatgatgaa cgtcggagct gcccagccaa agtacgtgtc tggaatacta cgattcgtac
    12961 ggtgatattg gcgcaaactt aaatagtgca aacacaccct agaagagata ggagggaatg
    13021 gcactttctc gcgttgttgg tggaagtatt tgggatgcta agcgagttgc cgcattggaa
    13081 ccagacgatt gcgtgaagtg accctatacg taaaaggtct cactggcaag agccagtgag
    13141 acctttgtct gatgcgatgc gagcgaccca ataatctcaa tcagacacag agccaacggc
    13201 cctgggcctg caattatgga ctcgcctttc ggcgaggctc aatgctggcc tcgctgctgt
    13261 cgcagctcgg cacagggcct gctgaaagcc caccgggctt tcaggaggcc gatcgcggct
    13321 aaataatgcc gcgatcggcc aacggccctg tccagtctgc gtcctcaggc tcgcgggcgg
    13381 actcgattct ttcttcggcc cagtgtttgg cggggatggt cttgggcttg aaggggaact
    13441 tttcggcgcg catcttttcg taggcggcga tgtcgtcctc gtgctgcagc gtcagatcga
    13501 tatcgtcgta gccgttcatc agacgccaac gcacgtaatc gttgacctcg aacggcaggg
    13561 tcacgtcgcc gcaagtgaca gtgcgggtct ccagcgagac agtcatctca cgaccaggct
    13621 cttcctccag tagcttccac aggagttcga cggactcctg cggcataatc gcggccagca
    13681 cgccgttctt ggcggtgttg ccgtagaaga tgtcggcgaa gctcggcgcg atgaccacgc
    13741 ggaaaccgta atcgtgcagc gcccacacgg cgtgctnnnn nnnnnnaatt cggggccggc
    13801 gaccagaatc tggcccttac cggcgtattc cggcttgttg agcacgaagt tcgggtctcg
    13861 gcgccatgcg tagaacaggg cgtcatcgaa gccggacttg gtcacgcgct tcaagaacac
    13921 agcgggaatg atctggtcgg tatcgacgtt ggagcggcgc agcggcacgc ccacgccggt
    13981 cagtgtggtg agtttttcca taacaatttt cctttcgggg atctctcccc cagtcagtct
    14041 tcgactgaca gcccctcgtc agagggggct cacgtgt
//
LOCUS       DAWBMW010000023        13939 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_1121_length_13939_cov_3.081245, whole genome shotgun sequence.
ACCESSION   DAWBMW010000023 DAWBMW010000000
VERSION     DAWBMW010000023.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 13939)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13939)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13939
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1121_length_13939_cov_3.081245"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2812 a         3991 c         4190 g         2946 t
ORIGIN      
        1 ggccaccgga ttctgcttgg cgagcaactt gacgcactgg tcaatcacag aggactccag
       61 ggcatcgatt tcgatgtcgc cgtcaatcac agtctgtgcg gcttcaacgt cctgattcag
      121 cagggcttcc ccagctcctt tgatcgcctt gcgtacgttc tgcgccatac ggtcgaggtc
      181 gtcagcaacc tgcttcagct cctcgttgaa aataacgcgc atttctctag cctttcatgc
      241 ttgcttattg caacctgtac cagtgtatta ttccagtact tgttccccac ttcaacgtag
      301 gtaatggcaa cctcaagttg ttctactgtt cattgggttg ttcacctgaa attcatgtta
      361 ccggcgtgcc ggcaaaccgc aatcccggcg tgtggtgtga aagaatgggg gcatgacaca
      421 gatgccgctc gtcgccgcga ttgctcttat tgtcacggct gttttatttg tggtaggaat
      481 catcacgctc atcgtgggat ttgcgacaca tcgagtgtcc atggaatcgc cgctggccga
      541 tgatgacgac aatgacgatc tgtccgacga caccgccacg ctgctgtcca tgctgccagg
      601 tgcgtccgtg gtggtcgacg agcacgacga ggtggtccgc tgcaatcccg ccgcctaccg
      661 cctgggtgtg gtgtcagatg acgccattgc ccagcagcat gtgctggatg ccattcacga
      721 ggcgcgcaag tccggtggca agcgccagtt cgacttgacc accgatactc cggagcggta
      781 cgtcgcagac cagaacaagg gcgaccacgt cgccacgcag tccgtccacc gccccaactg
      841 gctgaaggtg actgtagggc gtatcaacga gcagttcgtc atcgtgttga ttaccgatgt
      901 cagtgatgtg attcgtttcg ctcaagtgcg tgactcgttt attaccaatg tgtccgagca
      961 attgttgggc cccactgagg ctctggccaa actcgccgat tcgctggagt ccggcaatct
     1021 ggacgaacag caggtggctg ccgacgctcg ccaggtgcgt tccagctgca gcaagctcaa
     1081 ccacatggtg tctgatttgc tgctgctcat acgcgcgcag gagccgatta cgccgtcttc
     1141 cgccaaccga ctgaacgtta tggaacagct cagggccacg gcggcgcgac tggagccgca
     1201 ggcagccgag gctggcgtgc agctgaacat caagggcgac gatgatctag tcatcaatgg
     1261 tgaggcggac cagatcgaca gcgccgtgac caagctcatc gaaaacgcca tcggctattc
     1321 caaggaaaat ggcgtggtca gcgtgtccgc atccaaagac aaggatggcg accgcgcggt
     1381 gattcgcgtg attgaccaag gcacgggcat cgctaagaag gaccaggcgc gcatcttcga
     1441 gcggttctac cggggcgtgg aacagagcaa tcgcacggcg gatggcgtgg gcctaggctt
     1501 ggccatcgtc aagcatgtgg cgctgaccca tcatggcgat gtgaccgtat ggagcgcgcc
     1561 cgggcaaggc agtacgttca gccttacgtt gccattggcg cagtgaggta aggaattggt
     1621 ggcatgcctt gcatgccacc aatgctgttt gatgagcgac ctgcggccgc ggtctctccc
     1681 tcaatcagct acgctgacag ctcccgtcat cagagggagc ttgtgttgcg ttactgtccg
     1741 aggcggcggt agtcccagcg gtcgaagtat tcccagacca gcagtagcaa gccaataatg
     1801 acgccggcca cgcacataat caccaacggc attacgctgt agcccacggc cagcagaatc
     1861 agcacgacaa tcaacgccac aatccataga ccggtaccga tggcaaacac cttacgcaga
     1921 tccacccgta cgggcttggg tgcgggtttg cggacggcag ggtcaatgag aggcgcgaac
     1981 ttcatactca tgcactgtag taagagtagg gaggggcgtc aaacatccct ccctacgatt
     2041 tattcaatcg gcatcagttg agaccgatca atgtgcatgg catatatggt cggcaatgcg
     2101 gggcatatat gccatcacag gtgctcgacc acgtagtcga cgcacttggt gaaggccatg
     2161 acatcagccg gttccaccga ggggaacacg ccaacgcgca gctggttgcg gcccagcttg
     2221 cggtagccgg cggcgtccac aatgccgttc tcgcgcagga tggacagtac ctgagaggcc
     2281 tggactcgct cgtccagatc aatggtgacc acggcgttcg agcgtgcgtc cgcatcgacg
     2341 acgaacggag cggcgtattc ggagcgctcg gcccacgagt acaggatgga tgcggacttg
     2401 gcgcaacgtg tggtggccca ggcgaggccg ccgttgttgt tgagccagcg aatctggttt
     2461 tccatcatga tcagggtggc cacggcgggc gtattcaggg tctgatcctt gcgggagttg
     2521 ttcagtgccg tggtcagcga gaggaacggc ggcacccagc gatgtgcgcc ttccagatga
     2581 gcgcttgatt cgacgcctgc cgcacgatcg atagcttcgg gggagaggat ggcgacccac
     2641 agaccgccat cggcaccgaa ggccttctgc ggtgagaagt agtagacgtc ggtctggctg
     2701 atgtccacag gcaaagcgcc ggctgcgctg gtgccgtcga tgacggtcaa ggcaccagct
     2761 tcacgacttc cggccacgcg gcgaatcggg gcggccacac cagtggaggt ctcgttgtga
     2821 gcccagcaat atgtgtccac gccctcggtg agctcaggca gacggtaagt gcccggctcg
     2881 ccggggaaca gcaccgggtc ttcaaggaag ggtgcgttcg cggcggacgc ggcgaacttg
     2941 gcactgaacg agccatacgt gccgaatgcg gcgcgacggg tgatcagcga agcgcaggca
     3001 atctcccaga acgcgctggc gccaccgttg ccaagcgcca cctcataccc ttcaggcagg
     3061 tggaagaact cgcgcagacc ttcacgaatc gaagcgacca gttgcttgac tggtgcctgg
     3121 cgatgtgatg tgccaagcag ggtggtggcg ccggcgtcga gcgcggcaat ctgttcgggg
     3181 cggattttgc tggggccgga gccgaagcga ccatcttcgg gcttcagcga ctcgggaata
     3241 ctcagtgtga tagtcatggt ttcaagggta gaccagtgtt ggggaatatc gcggataatg
     3301 ctcggtgtgc gggattactg cggggttact gcggaattcc ggggtgccgg gcggcagtgg
     3361 tggcaatgcg gaatgcagtg cagaatgtac gcagcacatc atggttacgg tgtgtcggaa
     3421 tgacatgagt tagtgattct tccgataacc ggatagtgag gttcgtgaac gggcggaaac
     3481 aaggcagttt tttcacccgg ccgcacggtt acgtaaatcg tttgacacac ccggatggtg
     3541 ggggatcaca ttgaggattg acctctgttc gttgtacagt actcaattgt gacggtcata
     3601 cgaggtcatc gaaatgaccc cgccgaaccc atccaaggag ttaacgtagt atgaagcatg
     3661 cttcgcataa agcgaccacg gtctcgcgcc agaagttcag cttagcgaag gcattgtttg
     3721 cctccggcaa gggttctcat tccgttcgtg ctgtgcgtgc ggtgcaatcc gctggtagcg
     3781 acgccgttgt tttggggctt gatccggcca ttgccgagaa gatcaacgaa atcgctcccc
     3841 agacccgccg ttccattcgt gaggccgctc gtgccgctga acgccgtagc catattcttg
     3901 cctcggcctc actggccgct ctggtcggca cggccgcagg tgctctcgcg ttttccaaca
     3961 atgaagacgc cacgaactcg ttcgtggctg atccggccac gacgaccacg cagattaagc
     4021 gcatcagcga tgtgactgcg gcttctcgtt ccgaagcgcg tactgatttg agttcatcct
     4081 ccgttaccgc acagtccacc aatgaaggcg gctgggggct gagcaattca gacagcacct
     4141 tggacgccaa gctgatgaat cgcaataatg cgaacaatcc tgtggtgtcg gctctcgtgg
     4201 atgccgatgg cgatgctgtg ccggctggct tcaacccgaa ccatgccgtt ggcaccaact
     4261 ccaacaacta tccgtggggt cagtgcacgt ggtacgcgta ccagcgtcgt gccgaactgg
     4321 gcctgccttc cagcggcaac ttcggcaatg gtggcgcgtg ggcggcgtca gcttccgctc
     4381 tgggctattg ggtggacaac actcctcgcc atgtcggtga tgctgtggtg ttcgctcctg
     4441 gccaggccga tgccgatggc gcgtacggcc atgttgccgt tctggaaaag atcaatgccg
     4501 acggctccat tgagatctcc gaatccaatg ccaagggatt gggtgtgatt tcgacccgta
     4561 cgttcagcgc cgccgaagcc ggtaagttcc agtacgttca ctactgattc atttcattgg
     4621 cttacattcg gtgattactg ttcggatgtg acgtcgccct atcggcaatc gttcgcgcga
     4681 aatgacattt caagtgttgc ggccgcgtat tagtccgaac tgacctgtgc cgacacgccg
     4741 atatatccca atagctccgg ggtgttttat ttcttccatc ctgtacgagt taatgcgcac
     4801 cgtgtagggt gatggttcta tgaagaccaa aactgtagct tcttccattg ttgccgcgat
     4861 tgtctcctgt gcctgcttgt ttatgtttgt tcctacagca actgcagctg agggtgttac
     4921 tgtgacttcc accaagtcct tcccctcgta cacccaggtg cgcaaagatc tgacgactga
     4981 agccactgcc acgagcgttg aggacaatgc tgattggggc ggcattgaga acctcgatgt
     5041 gccgcagacg gaatctcagg cggaaaagga tgccgcagcg gccaagaagg ctgccgaaga
     5101 ggaggccgag aagcaggcgg aggtcgaggc tcaggcgacc caggagcagg ccaacgccgc
     5161 gagccgtaat gagtctcgct cttctgttac ttattccgat gccagtggtt cggtttccgc
     5221 tggtgccggt gcctctatcg accgtgcgta ttctttgatc ggacagagca tggactgcac
     5281 cgcattggtc agtcaagcgt tggcggcccg tggcattaac ttccacggtt ggcctgaaga
     5341 gtacgtcaat gttcctggtg gccacgtagt cacagacgga tccctgcagc cgggcgatat
     5401 tctgatttat gccaacaccg gtggatggaa cggtggtgca cactacgatc atgtggcgtt
     5461 gtatgtcggc aacggtcagg cggtccatgg tggatggaat ggctatagcg tggctctcgc
     5521 tagtgccatg accgaaaagc tcaccatcgt ggtgcgtatc ccgtgatgca gtcttattga
     5581 ttgatttatt gcaggcctcg gatgccgatc ggtatccgag gcctgttgcg ttctcctgcc
     5641 gaatgatgca cccgcgacat catcttcgaa atgctattcc tgttatgaaa tcgacccatg
     5701 tgtgctagtg tatggcgttg atgatgagcg ttaagactat tatttccaca tcagtggcga
     5761 ttatcgccac gggtgccatg tttgcgtgcg tagccccgtt tgcctctgcc gattccgcgc
     5821 agacgagtgc tgtggtgtcc tcacgttctt tcccgaaggc gagttcggtg aagaagaatt
     5881 tgttcgccga atccacctcc acctcggttg atgagaactc taaatggggt ggcatcgaga
     5941 ctcttgatgt gccacagacc gagtctcagg gcgagaagga agctgctgca gcggcggcgg
     6001 ctcagaaagc cgctgatgaa gctgctgcag cccagtccgc tgctgctgcg gcagccagcc
     6061 gctctgaaca gcgtgcgtcg attccacagg ttgacttggg taaaatgact ggcactggca
     6121 ctggcgctga gcttgctagc ttctcactga agttccaagg ctatccgtat gttgccggcg
     6181 gcaacactcc ggcaggatgg gattgctccg gttttgtgca gtacgtcttc tcccagtttg
     6241 gcatctccct gcctcgtacc tctggtgctc aagccaccgt gggtactccg gtcgcaagct
     6301 tggcggatgc caagccgggt gacattcttg cgaatggtac tcatgcggcc atttacatcg
     6361 gtaacggaca ggtgatgaat gctatgaacc cggtgcaggg aacggcagta agcgacgttt
     6421 ccgtattcgg tggagctgga tattccattc gccgcgtgat gtgacaagaa ttatgcggta
     6481 agcgaattcg tattaaggca cgtcctttgg ggtgtgcctt tttcgtatac gcggatttct
     6541 ttttgagcgg aggataccgg ccaaaccgag tgtcgcgggg tattgttgag atacgcggta
     6601 ttcgtgccgc aataacttca aggaggtcgt catgattaac gacaaggcta ttctcgtcgg
     6661 cgttgacggg tcgcatgcca gttacaaggc aacatggtgg gccgcgaatt atgcaaagca
     6721 cgcggggctg acgctgcaga ttgtctgcgc atattccctg ccgagctatg ccgccgtttc
     6781 gttcgatgcc acttacacgg cgatggggga cgataacgcc gcgcattccg atgctcagga
     6841 gattctgtcc aaggccaagg ccatcgcaga cgagcagggc gtcgaagccg ccacactgat
     6901 tgttaccgga gatccggcat cggtgtttgt cgaattgtct cgtaactaca atctgatcgt
     6961 cattggcaac cgtggcaagg gcggtctggc cgagcgcctg ctgggcacta cgtcctcatc
     7021 gctgccggca tatgcttact gcccgattgt ggtggtgccg tacaccgatg atgacggtaa
     7081 cctgatgcac ctgaacaaca cgatcaccaa ggtggccgtc ggctccgacg aatccaagtg
     7141 gggtctgaag gcgctggaca ttgccgccga cttcgccacg tgctggggtg cggaactgga
     7201 cgtgatttcc gctgtgccga acctgaaggg tgtggacggc gaggacgccg tcatggagtc
     7261 ctacaaggac gatcttgagg tgcgcatcaa gccgttgcag gaatcgcatc ctgatctgaa
     7321 gatcaacaag cagatcgttt ccggcccggc cgtgagcgcc ctgaccaagg ccagctacga
     7381 tcacgatgtg gttgttgtcg gctcccgtgg tcgcggcggc ttcactggcc tgctgctcgg
     7441 ctccaccagc cagggtctgc tgcagcatgc ggtcggcccg gtgtacgtgg tgccacgcaa
     7501 gtatgttgag gccgcggaat cgcgtctcga caccgtgccg agctcgcccg ccgaagtgcc
     7561 taccaagtcg ctggaggaga ttgccggcgt cgaggagatt ccggtgtccg cagccgagcc
     7621 tgaagtcgcc cagcagatcg aaaagaccat cgatcccgat cgccagtaaa gtccgaatat
     7681 acggaaagag gctcccgcca gggagcctct tcaatttata tcccgtctca gtgcttgacg
     7741 gtgacgttca tgcgcaccgg ggtctcctgc acgtaggtgt gcttgaccgt gcaacccttg
     7801 tcgatatggc gggtgacgcg ctccttgagc ttgtcggcat cctcctcgga cagaccggcg
     7861 tcggaagcat caatcaccac ctgctcctcg aaaccggtgt aggcgtcatt ttccgggtcg
     7921 tacgtgccat ccaccacaat cttggcaccc ttgccctcac ccagcgtatg ttcgatggcg
     7981 aactggctgg acagcgcggc gcaaccggcc agcgcgacct tcatcagatc gcccggcgtg
     8041 aactggccac gatccttgcc gaacttaatg tgtgcgccat cgtcgctgaa cgcatcccac
     8101 gaaccgtcct tgttacgctc aacccacagt cgcttaccca tagctgctcc tttattttca
     8161 gccgccggcg atctggccca tcgccatgcc agcgggggag cgcggcacct ggccgctctc
     8221 ccgcatcagt ggacttctct attatgcccc taagctacac tggtgcacat ggctttgacc
     8281 gaagaacaac tcgcagatat tcgctcccgt atccccgcac gtcccgaaac ggacattccg
     8341 atgccctatg aggacctggt gcgcaagatc ctgaccgaag gcacgctgaa gtccgatcgc
     8401 accggcaccg gcacgatttc cctgttcggc cagcagatgc gctttgacct gtcgaagtac
     8461 ttcccgttgc tgaccaccaa gaccgtgttc ttcaaaggcc tggcctatga gctgctgtgg
     8521 tttttgaagg gttcctccaa catcaactgg ctgctggagc acaacgtgca tatttgggat
     8581 gagtgggcgg acgagaacgg cgatctgggc ccggtctatg gcgtgcagtg gcgttcctgg
     8641 cccgcgccga cccccgagga cccgaatcgc accatcgacc agatctccaa tgtgctcgac
     8701 ctcatcaagc atcatccgga ttcgcgccgc atgatcgtct ccgcgtggaa tccggccgag
     8761 gtggagaaga tggcgttgcc gccctgccac gcgctcttcc agttctacgt ggccgacggc
     8821 aagctaagct gccagctgta ccagcgttcc tgcgacatgt tcctcggcgt accgttcaat
     8881 atcgcctcct actcgctgct caccatgatg atggcgcagc aggccgggct caagccgggc
     8941 gaattcgtct ggaccggcgg cgactgccat gtttatgaca accacgtcga ccagttcctt
     9001 gagcagctgg gccgcaagcc ctacccgtac ccgaccatcg agatccgtaa ggccgattcc
     9061 ctgttcgact accagtatga ggacttcaag gtcgtcggct accagcatca ccccactatt
     9121 aaggcacctg ttgcggtctg atgctgatta ccgtttcgtg gacagcgaat actgtgaagg
     9181 tgacggcccc cgagtcttcg ctgtccacta cactaaggcc cagttccgca atccaggagg
     9241 agtaagcgaa atggagcatg acagtagccg tagcggctat cacgaacccg agcccggatc
     9301 tgccgggctg tctgccactg aagaagactg gggtgacgat tatcccaaga ccttctcggt
     9361 gaatcttatc tgggccgaag cccgtgacaa ggaaggtcgc gccggtgcca tcggcctcaa
     9421 tggtggcatg ccttggcact gtgccgagga tatgaagcat ttcaaggagc tgaccgtctc
     9481 ccatccggtg attatgggtc gtaagacctg ggaatcgctg gggggcaagt accggccgct
     9541 gcccaaccgc gacaacatcg tcgtttccca cgatccgatg taccgtgcgc cgggggccac
     9601 tgtggtcacc agtcttgacg acgcgctcga tatggcccgt caggaggcga ttccggacga
     9661 cggtctggac cgttcggaga tttggatcat cggcggcgcc cagttgttcg ccaaggcttt
     9721 gccgttcgcg gacaaagcct atgtgactga tctggccgcc accgtggatg ccgacagcta
     9781 cgcgcccgat atggcgtcgc tggtcgaggc cgggatgtgg cgggaagccg aagtgggcga
     9841 gtggcatacg cccgccaagg aggagtccgg catcgattcc tatcgtttcc gtatcttggc
     9901 caaaaccaag taagcatgcg aatccggctt atgcccttta tagcggcatg agccggattc
     9961 atattcattc gaccccaaca atacagtcgg gccgtgtgcg gcccggtgaa gcccgattga
    10021 ggattgattc aaagctatga gcaataccca gccctacacc gtaatgaccg tctgcaccgg
    10081 caatatctgc cgttcgccta tgggtgagat cattctgcgt cacttcttta atgagcgcgg
    10141 tcttggcgac caggtgaacg tggaatccag cggtgtgtcc gacgaggagt ggagccaccc
    10201 gattgacccg cgtgccgtgc gcgtgctacg cgaacgtggc tatggtgatg agattccgcg
    10261 tgaccacttc gcacaccgca tctcgcgtga ggaaatcgac cgtaccgacc tgttcctgcc
    10321 gatgaccgcc tcccacatgc gcgccctgct gcgcatgctg ccggccggca agcgcgccga
    10381 agtgcacatg taccgcagct tcgacccgaa tctgcccaag ccgaagcccg gccgcgaaga
    10441 tcagatcgat ctggtggacc cgtggtacgg cggcaagcac gagttcgagg tcgccatcga
    10501 ccagattgag gaagtcgccc cgtacatcgt cgattgggtg gcgaagcagc tgtaggaatc
    10561 ggcctgtggc cgatgctgtg tgtttcgcag tcttacgact gcgggtctct acctccgtca
    10621 ccgcttcgcg gtgccgcctc cctcgtcaga ggaaggtaca gaagattacc tccctctgta
    10681 agggaggtgc tgagcatagc gaagcggagg gagagactaa cgagtcgggt gcctcagaac
    10741 accagttgca ccagcaacat atatacaatt gtgccgccgg cgattgaaag cagcatattg
    10801 cgtttccata cgtgcagcag cacgatgacg gtgcaggcga tgagttccgg aaggccgtgt
    10861 gaaccggtca gaatcggggt gttgcgcagt gcgtatacca ccagtagacc ggtcatcgca
    10921 tagggcagga cctggccgaa atactcgatg atgcgcggcg gctgcttgga ctcgggaaac
    10981 accagaaacg gcagaaaacg ggtgcacatc gtgcccagca ccaccgtcac gatagtgatt
    11041 gcgccttgcc agatggtcat cgtcatcgcg tctgtccttc cttctgctgg tccgctgcgt
    11101 ggctcatggc atggtttgcc gtggcttccg gggcgtcggc ggctggccgc aacacgtcca
    11161 gatgcgggcg tagcgcgacg aacagtatca gcatggcgat cattgaggga atcatgaaat
    11221 cattggcccc gaatatcagc aggcaggcca gtgatgtcag cacaccgatg acggcggaca
    11281 gtcgctcgcg atgcttgccg tcgagccact gatcgaggaa gatcaccaga aacagcgcgg
    11341 tcagtacgaa atcaagaccg tcggtattga acggcagatg cgcgccgatc agcccgccca
    11401 gtgtggcgcc ggtcacccaa tacagctgat tgcacagcgt aacccagaag tagaaccagc
    11461 cacggtcgat gccggcgggg attttcgcgg tggagttgat ggcaaaagtc tcgtcgcaca
    11521 taccgaaaat caggtatggg cgcttccagc cgagcccttt gaatttgccg agcatggaca
    11581 gcccgtagaa caggtgacgg gcgttgacca tcagggccag cagaaagccg gcgagcgggt
    11641 tgaacgcgga gagcagcaga ttcaccgtga cgaattccat tgaaccggcg aagatgaacg
    11701 cgctcataca catcggccat acgaaggaga agcctttggt gcccatgaga atgccatacg
    11761 aggcgccgag aaacaggaac cccaggcaaa tcggtatggt cagcggaaac gccgctttga
    11821 gcgccggcag ccaggcgttg gtgttggtgt tggcggtgtc tgccatgtgg atgatgttct
    11881 ccttgtacaa ctggtctgcc ggccgatgtt cgccgatgat gccatatgcg gttatacacg
    11941 catggtgtcg ataaacgggg tgagggtggc gtcgggaccc tgtgcgcgaa gtcacagtgt
    12001 ggaggatgtc acatcgtcta cggtgggata gtccttgtgg cgggcgcgaa cacgtgctga
    12061 gatgaaacca tgagcacata tcaagcgggc cgaatgcttt cagacctggt cccgcgaata
    12121 cgaaaagacc tcggtcgcta tgaccggggt ctttagtgaa gtacccccag agagagtcga
    12181 actctcgttg tcagattgaa agtctggtgt cctaaccgtt agacgatggg ggcgaacaac
    12241 tcgaattact ttacgggagg ttgttcggtt atgcaaatcg acggcatttc ggcgtgtcgc
    12301 aatcagttca gggtaacggc ctgataggtg aagtcatgaa tcacgcggcc gacggccagg
    12361 cccttcttct cgaagttggt gagcacgcgg ccctcgaatc gctcggattc ggtgaagtcg
    12421 gcgtgcggca tatcggcggc catgtcggca ttgcccttgc ccacgtgctc cagcggcagg
    12481 ctgacggtta cgctgcccag gttcttccag ccatcgaggc cgtccatcac ctcgtgcacg
    12541 tgcagggcat agtcctcgat gtcggtggcg atacgccaga cgccgtctgt gaccagcgcg
    12601 cgatgaatgt cgccggccat ggccttctgc acgatgcggc gcttgtggtg cttcttcttg
    12661 ggccaagggt cggggaagaa ggtccatacc tcggccacgg tgccggccgc ggtcaccttg
    12721 aacagctcgg gcgcattgac ctgggccacg cgaatgttgg tcagcccctg cttgccggcg
    12781 agcagcagcg tgtgggccac gccggggtca tagacctcta gagcaaggaa gttcgtctcc
    12841 gggtgggctg cggctgctgc caccacgttt tctccctgcc cggtgccgat ttccacaatg
    12901 agcggattat cgttgcccca tgcggactcc acgaacgcgc ggtcgagcgt gacgccttcg
    12961 cgcacgtcca gcaggttgcc ggccgcaata tccaacagat atgtgccggc atagttgtcc
    13021 catgcgcgct gcaggcgatc gtccaaacgt ccggagcggc gtacgaagga gagcaccttg
    13081 tgcagggggt gcgccggtgc ctcggtgtgg tctgtgccca tcgcggcgac gggatcgatt
    13141 gctgtggatt cgggattggt catatattcc tcggattttc cttgtgcgcc gcaccgctgt
    13201 tcattcatta ggtcgatttg gcgttcatta ggtaggtggt gtgcgatacc aaccaccata
    13261 tcaagccgtt tacccggtgt ccgtgcgggc cggctccggg ccgacctacc taatgaacgc
    13321 caaatcgacc taatgaatgc agatattgcc ggacgacacc accgttttgt gcgattatgt
    13381 tcgtgtgata tactcaaatt gtttcaaaag gtaacaaagc ggaccaaaag tgaacagccg
    13441 ctgaactccc cacggccgac acttgcaacc cgcacaagga aaggtcaact atgacagttc
    13501 tcgtcaccgg cggctgcggc tacatcggcg ctcatgtcgt tcacgcactc catcaggccg
    13561 gcgaaaaggt cgttgtcgtc gacgatctga gctacggcaa gcccactcgc atcgaaggct
    13621 cccgcctgta cggcatggat atcgccgccc cgggcgccgg tgagcgtttg gccgaaatcc
    13681 tcgatgccga aggcgtcgac tccgtgatcc actttgccgc tcgcaagcag gtcggcgaat
    13741 ccgtggagaa gccgctgtgg tactaccagc agaacatcaa cggcatgctc aacgtgctca
    13801 ccggcatgac ccagtccaag aacgccaaga agctcgtctt ctcctcttcc gccgccacct
    13861 atggtgtgcc gccggtcgat gtggtgcctg aggacgtcgt cccgatgctg ccgatcaacc
    13921 cctacggcca gaccaagct
//
LOCUS       DAWBMW010000024        13722 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_1146_length_13722_cov_3.104339, whole genome shotgun sequence.
ACCESSION   DAWBMW010000024 DAWBMW010000000
VERSION     DAWBMW010000024.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 13722)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13722)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13722
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1146_length_13722_cov_3.104339"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2836..2845
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6782..6791
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11652..11661
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    12717..12726
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2474 a         4003 c         4249 g         2956 t
ORIGIN      
        1 acatcggcca gatgaagatt ggccgtcgac tggccatcaa gctgctgaac gccaccaagt
       61 tcgcgctggc catcggccgt gaggacgaga accatcacgt gggcgctgcc gccgaggccg
      121 cgtggaaccc ggccgatgtg accgaaccgc tggatcgcgc cgctatggcc aagctggctt
      181 tggtggtgcg tcaggccacc gaggcgctgg aatcctacga gcattccaag gcgctggaag
      241 tcatcgagtc ctacttctgg cagttctgcg acgattacat cgagctggtc aagaaccgcg
      301 cctatggcac tccggacgag cacggcaatg tgccgtccga gaaggctgtg aagtccgccc
      361 gcaccgctct gggtctgggc ctggacgcct tcgcacgtct gctggccccg tacctgccgt
      421 acgccaccga ggaagtgtgg agctggatgc acgccggttc cggttccgtg catcgcgccg
      481 cctggccggt cgtggacccg tatgtcgagg ccgccaccgg tgcttccccc gagctgctga
      541 cttgggccgg caaggccgtg gagcagctgc gtaagatcaa gtccgaggcc aaggtctcca
      601 tgaagacccc aatcctgtct gtggcactgt ccgctgcttc ggaaggtgtg gaagcgattc
      661 acgccgcctt gggcgatatc gcgcaggccg gtcgcgtggt cggcaagttc gacttggtgg
      721 ccaagcacgc cgaggaatcc gccgccgaag gcacgcccga aaccgaggtc gccgtcgaag
      781 ccagcgagct cggtgagccg ccagctaaga agccaaagca ctgaacgtta cgcacaacaa
      841 cagtaggtct cacggcaatg ccgctcgttc cgagcctacg ggaagcccac cgggcttccc
      901 gcttaacggc tcggccagct aagaagccaa agcactgaac gttacgcaca acaacaaaag
      961 aggggaacct attgaggttc ccctcttttg ttttttgacg ttcggatgac acgactgtgg
     1021 gacggcacgc aaatatcccg catacaccat cacaccgtcg gaatcacgat gagatatcgg
     1081 gtttcggcgg ttttttgttg cgtattgggg ctgtcaagct gcgcgacggc ctgcgccaag
     1141 agcgtgccag catcactgat tttcacggtc tcattaatca gcgcgttcat atcgtgcttg
     1201 aacagcgtgc cgccagtgcc aaagccgaac agtactggag cggtgtcaag attgttgccg
     1261 gaatcgctgg cgtcggaggc cttgccgttg ccggaatcgc cagtgtcgct cgacttggct
     1321 gagtcgttcg ttcccgaacc agcgctctta gacgagtcgg acttatcacc accaccgatg
     1381 gccgaagtaa gaccacccgc atagttcatc acatcgccag cagacatgga catggtgtca
     1441 tcgaccagtg aggccttgga atgctgcgac tgctgctgtt gcgcggcttt ggcctgctta
     1501 cgggcggcat cggccttacg cttggcttgg gcgacccggg tcttgccgta gaacgattgc
     1561 gattgcgaat cgaccaggcc ggtcagtgcc tgagcctcgg tcaccggatc ggaagagaca
     1621 tcaccggacg aaccatcacc atcactgccg gaagccgagg acgcgccgcc ttcgaactga
     1681 tcggagaatc gcaatacaat ctggcgcaag gccgtggctt tgcccgtatg ctcggaggcg
     1741 gtggcaagcg tgctcatcgc ggcatcgacc ttggattgag cgtcggcgag ttcatcgcta
     1801 ccgttggttt cggcgatggc ttggctctcg taagcggtgg cgcccgagga cgtgccgccg
     1861 gtcagggcgc tcaaattggc cgtgtaaacg tccgtgtatt tcatcgaata cggaatcatt
     1921 gcggcggaga gctggctctg gccatcggcg tattgcttgg agccttgagt ggcttcctcc
     1981 gaagcctcgg cgatcagatc atccaccagt ccggtggtgc cttgctgctg gatgcggcga
     2041 atcacctcgt ccaacgcatc gaccgcaccg gcatgctgat gtgcggcact gacgttgttc
     2101 accgcgttgg ccaagtcgag tacggcttga gccaaaggcg tgtccccgga cagttcgccg
     2161 gccgtgccca tcaacgccgg caactgggtg gaggtgccaa tcgatccaga caggtgcgtg
     2221 gtatatgagc ccacatacgc ggccatatag tcttcaaacg tccgcttatg ggcggtttcg
     2281 cgttcggcca ctacagactt gttcagcgca cgttcgttat cgcgcagggt ttgaagctgc
     2341 tcgatgagct gttgctgttc accagaaccg gcgtcggtca gtgaatcgac cagtgtggcg
     2401 gcagaggcgg tgagttgggc gacatccgta tcggcagcag gctgtgcggc agcattcgca
     2461 gcattcgcgg catcgggatt agcaccaact gccggactgc tggaattgga aggcaatacg
     2521 gcgagcgcca cggaactcat ggagaagtct ttggcgttca tatagcagct gaattccaag
     2581 ccggttgcgg cttttccagc tgcggcgact acggtattgg aaccttgcgt ggtcaccgta
     2641 atggagttgt cagcggaaac atcatcggct acctgcgtag gcacggtaaa ggcgacaacc
     2701 gggatcgtac ggttggcttc cttggtttgc ggcttaatat cgatatacac gccgatcagt
     2761 ccagacgcgc cggcaacctc gtttgcctcg acgttcggac cgtcaagcga gtaggtgacg
     2821 ctgactttcc atggcnnnnn nnnnntggca gggtggcggc ggaagcgggg atacgagaaa
     2881 ccttggacgt gccggaatta ctattggcgg aatcggtatc agtggaaccg ctattgccgg
     2941 aatcggcact ggcgccattg ccggcgccgc ccttccctac cacataccaa cggaccggct
     3001 ccccttcggc atcggtgacg gcgtacatat cacttgccgt agcgagcacc ggaccgtaat
     3061 caaccggagt cggacgagac atccaagcac cgccgagaat cgaagccacg cacaatgcgc
     3121 aactgaccag tgcggcacca catctcatct acgtatccca ttcatacagt cgaccactac
     3181 gggcattcca ttcccgtatt aatgtagtcg ctgcaggcaa cttgccctat agacgaatat
     3241 ccgaaaggtg cccattcgct gcgggcgaat caggcgaata cgtcgagtac gcccgggtcg
     3301 ccaccggcat cggctatgcg ctggtggcgc ttcttggctt cggtaaccac gaattcacgg
     3361 tacatttcgg caatttccgg atcaagcccg gcatcgatgg cgatgcggtg gaggcgttcg
     3421 atctgatagt cctcacgctt ggtgtcttcg ggcgcgaatc cggcgttggc tttgagcacg
     3481 ccgacctgcg aggtggcctt gaaccgctcg gccagtaatg cgatgaccgc cgaatcgatg
     3541 ttatcgatgg tttggcgcag cgctttgatg cgcgccaccg cttgtgcggt ttccgggtgg
     3601 gccgccgcct gggcggagtc gatggtagtc ctttgccagt cgctgctcgt ttcgctcata
     3661 gattcgagga tactcccgat tacggctttg cacgatgcgc aaagtcagat atctcagatg
     3721 tcatagtcgc cggtgaggat atggctggca tccgcatcgc cgaacatggc cgcaccactg
     3781 cccactccca aaacaatcga acccagtgat gcctctttca tctcatatat cgtcagcgtg
     3841 catgtgaacg ttgacggcaa tactacaact gcaatgtgat agttgatgta tttgcatcag
     3901 atacgacaag ggctgcaccc cgtatcaatc agggtgcagc ccttacacta ttgttcagcg
     3961 gtctgatcag ttgccgttga ccgcgttgcc ggtgttggcc tgctgcacga tggcggcgag
     4021 gaagtcgcgg ttggtggcgg tcttcttgag gcgcggcacc agcgtctggt aggcctgctc
     4081 gggctccatg ccgcccagca gtcgacgcag acggtagata atcggcagtt cctgcgggtt
     4141 ggtgatgagc tcctcgcggc gggtgccgga agcgttgatg tcgatggccg ggaacagtcg
     4201 cttgtccgcc agctcgcggg acaggcgcag ttccatgttg ccggtgccct tgaactcctc
     4261 gaaaatcacc tcgtccatct tggagccggt ttccaccagc gcagatgaga tgatggtcag
     4321 ggagccgccg ttttcgatgt tgcgggccgc accgaagaac ttcttcggcg ggtagagggc
     4381 ctgcgcgtcc acaccaccgg acaggatgcg gccggaggca ggggctgcga tgttgtaagc
     4441 gcgagccagt cgggtcatgg aatcgagcag tacgaccacg tcctggccga gctccacaag
     4501 acgcttggcg cgttcgacgg ccagttcggc aaccgtagtg tgatcggagg ccggacggtc
     4561 gaaggtggag gaaatgacct cgccctggac cgtgcgctcc atatcggtga cctcttcggg
     4621 tcgctcgtcc acgagcacca ccataaggtg gacctcgggg ttgttggcgg cgatggagtt
     4681 ggcgatgttc tgcagggtga tggtcttacc ggccttcggc ggggagacaa tcaggccacg
     4741 ctggcccttg ccgattgggg aaacgatgtc catgatacgg ccggtgagct tgttcggggc
     4801 ggtctcctgc ttcaaacgct cctgcgggta cagcggcgtg agcttggaga actgcggacg
     4861 gttgagcgct tcttcaacgc tttggccgtt gatggaatcg atggcctgca gcggcacgaa
     4921 cttctggcgc tggttgcgac ggtcgccttc acgcggggcg cgaatcgaac catgcacggc
     4981 atcgcccttg cgcaggccgt acttcttgac ctggcccatg gaaacgtaca cgtcgttcgg
     5041 gccgggcagg tagccggaag tgcgcacaaa agcataggag tcgagcacgt cgacgatgcc
     5101 ggccaccggc accagatcct cctgcggctc ttcgcggcgg ctatcacggt cattgcgatc
     5161 gctacgatta tcgcgatcat catagcgacg atcatcatcg gcgttgtcgc gatcacggcc
     5221 acgcatgcga cggttacggc gatcgctgcg atcattacgg gtatcgcgat cgttgcgatc
     5281 gctacggtcg cggcggttac gacgcacgaa ctcgtgatcg tcgttctgct cgccgtcttc
     5341 ggacgtttcc tcgtgcttct gcgtgggcag cgtggccagg atatcgtcga ggtcacgcac
     5401 ggtttcttcg ctgttttccg cagtgcgacg acggcggcga ggctgctcgt cctcatcatc
     5461 acggactcgg cgaatcagcg gaacttccac gtcctcgtga cgctcgcggc gacgctcggt
     5521 aggcgctgca tctcccaaat cgagcgtggc cagcagatcg ccggcctcat cggccttctt
     5581 gcgcggcttg cggcgagcga aatcgggctg gtcgtcatcg cgacgacggc gggccgcacg
     5641 cggttcggat gcggcatcat cggcggcctt acgacgggtg cgtcgcggtt cggtctcgtc
     5701 cttcttggca tcgtcggcca cgggaagcgg caaatcgagc tgcggttccg tggcagccgg
     5761 agccggagcc ggcgactggg tggccacgtc agcattgtct gtggtgttgg cctcagcggc
     5821 atcggactgc ggagccttga cgaccttggc ggttttggcg gccttggggg cgcgcacggt
     5881 cacaccggcc ggcggctctc caccattgcg cgccgcctgc agggtggcga gcagctcggg
     5941 cttgcgcatg gtcgacgtgc cgcgcaggcc catctgttta gcgagctctt tgagctcagg
     6001 cagcttcata tcctcaagat tctggctatt tgccactggt tgccctttcc ataccgaaca
     6061 gacccatgcc tatccaggcg ttaagagtga ttattgcggt tgcaccgctc atgaatgttc
     6121 gctgcgtctc tgagtctctg cgaacttcgt tcactgtaca acgccaactc gaccaacgca
     6181 aaacggcccg ccgaaaacca aaaaagctcc ctctgcgacg agggagctca acaaggttta
     6241 ttcacttttc ttcgtggtag gacttttcgg tgttgacgga ccacacgttc ttcttggacg
     6301 tcttgccggt cttgatgttg ccgacggcct ccatggcggt ggccatggag tggtccatgt
     6361 tgttgtagcg gtgctggccg ttacggccca cgcagtacag gttgccgaag ccgtcgaggt
     6421 actcgatgag ctcgtccatg tgctcatagg tgtcgaagta ggcggggtac gccttcctga
     6481 cgcgctcacg atgggagtcg atgacctcgt ccggaccgtt gatcacacgc atgcgggtga
     6541 gctcggaaat cgcgaacttg gtgacatcct cctcgctcat gttccagaac gagtcgccct
     6601 cctcgcagaa gtactcgaga ccgatccaca cggtgtcgtc cacgtccttg accaggtacg
     6661 ggctccagtt gttgaagatc tgcagacgac cgaccttgta gcccgggtcc tgcacgtaga
     6721 tccagcagtc cggcacgatc ggcggattgc cgagggtggg gatgctggtg gtgttcttga
     6781 gnnnnnnnnn ngtacggcag gccgttggcc acctcggtca tatcggccgg ggcgggcttg
     6841 gaatcaacgg cctcgggatc ggcgccggcc gcgtcgatgg cgttgaccag gtccttgacc
     6901 ggcatcgagg agatgaactg gtcgccggaa agctcggtgc ggttgccctc ggaatcctcg
     6961 tacaccacgg aagacacggc accatcggtc tggcggacct cgaccaccct ggcgtcggtc
     7021 agcaccttga cgccggcggc ctcgcaattg gactcgacgg tctcccacag ctggccggga
     7081 ccgtacttcg gataccagaa ctcctcgatc agcgaggtct cgacctccgc gttggagcgc
     7141 ttcttgggca gcagcttctg gaaggcgttc ttgagcacgc ccatgatgga caggcccttg
     7201 acgcgctggg cgccccagtc ggcggagatc tgggaggggt ggcggcccca cagcttctcc
     7261 gtgtagccct cgaagaacat cgaatacagc ttgcggccga aacggttgat gtagaagttc
     7321 tccagattgt cttccggcag cttgtggaac atggacttca ggtagctgaa gccggccacc
     7381 atggtgagct tgaagcccat ggccttcaac gtgttggggc tcagcgaaat cgggtaatcg
     7441 aagaagtggc ggttccagaa gatacgggac actcggtggc gcttgagcat caccttgtcc
     7501 tccgtctccg gatcggggcc gcccggctcc atgtcgtggt cgcggccgag cttcttgtca
     7561 tcataggagg gtgcgccctg caacggcaga acggtcttcc accaatccat gatgcgttcg
     7621 tctttggaga agaaacggtg gccgccgata tccatgcggt tgccgttgtg cttcacggtg
     7681 cgcgagatac cgccgaattc gcgggtcccc tccagcacgg tcacgtcgta cttgtcggca
     7741 ccgccgtcct tgatgagttc ccatgcggcg gtaagtccag ccggaccgcc gccgataatc
     7801 actacggatt gcttgtcggt cattatgctc cttataaacg caaaatcaga gacagtctac
     7861 tagaagcccc ggaatcgcac accgaaagat gagcgaatgc ttacagaatt ctcccgatat
     7921 acgaccgatg cgccgtcttt ccatcagcag aaaggcgacg catcgaatcg tgaggtaggg
     7981 gtattcggca acgttgcgtc acacacagcg cggcgaatcg gtttggtctt aggctaattc
     8041 gtcagcgacg aactttccat agattcagcg agatgcctgc aagtgcaagc agcatcataa
     8101 tccccgcaac accagtaacg gcaacaccag tacgactcaa ctcgttttcc gtaccattag
     8161 cctgagtgtt gctgggctga tgagttcccg caccattgtt attcggagtg gtactcgttc
     8221 cttcaccagt gttaggtcct gtgcccggct gcgttgtgga atcatcagtc agatgcgcat
     8281 cgtcgcctag ggtttggtgg tcggaggcca agcaactttt aagctcagtg agcttgtacc
     8341 acgtggaacc atcggcaatc ggcgtgcatt cggagcggaa ggcgatttcc tgctcgttgt
     8401 agagcatacg aacgagcggc gtgtaggcac ggccggtctt cgggtcgttg ccggatttga
     8461 cggcaacatc ccactgcaca ttagcggcca tcggagtcac cgattcaccg cgccattcat
     8521 tgttggcgta ggtgtacacg tcggtggtgc tggcggccgc ctgctgggtg gaaccgggaa
     8581 ggccaagcag cgcggcgaac ggcatcatcg tttcggcatg ggcaaagcgg aaagtagcaa
     8641 cggtggatcc gccgtttacg cgctcatcaa tcgacgagaa gaaatcgtca agcagcggct
     8701 gtgcgattgt gtaggtctcg tcttggccgg cgtagctcgg gcccttctcg tagaagtcct
     8761 cggcgtcaag cgcccaggcg aacaacttgg cgtcgtctgc ctgctcacct tggaagtact
     8821 ggttgaagtc gaaggtatgg ctgccggtgt tctcgttctc catatcagcg gcgatgatgt
     8881 acaggttgta cagatccatc gcggcatcgt attcggaggc gatcttcttc ttggctgcgc
     8941 cgcaggcgtc ggcatccttg ctggggtcgg ctcccggcgc gcagttggcc tcgccgccct
     9001 tggtgccatc gacagtgttg taccaggcgt acgtgccgtc ggccagtttg gcgaggaagt
     9061 catcggtgaa gatttgtgaa agcaaatcgt gagaggctgt tttcacgtcc tcgttttcgg
     9121 cgatggtttg ctcagcgcct gcgataatcg catcgttttc cacgaagttc tggtaatcgt
     9181 cggcacgctg cttggcctca ccggttttct cagtgccgtc cgggttttcg gacttgtgga
     9241 agtacagcgt gttcggcgtc ttgtcgaaag ccgccgcctc gccggtgccg gccggatcgg
     9301 cgggcgtcac agtgctgttc gccagtttat tgttcgaggc cgcgttgaaa ccgcgagcaa
     9361 aattctcgcc tgattcagta gcgcgggctt cgccagagga ctgataggca atcttgccac
     9421 cgtccttggc tgcgttgtcg aacagcgtct tgttgcgctc gtaggcacgt gcgccaatgc
     9481 cctgatgttg gtcagcaccc tgaccggtga gcatagcgta gccattctcc acatttgcgg
     9541 cggtgatggc tttgagattc ttcatgaatt cggacttgat ggcatcagac ttaaaaccgt
     9601 tatccgcttc ggcggcttcg gccatcttca taagcaatgc gtcgtatttg tagctggaca
     9661 ggccgcgtga accgtggcga gccatcgact cggtgtatac cgtttcgtag ccttccggcg
     9721 cctggctata gctggcagtg gtcgcttcgc taggtgccac atatggctgc ttgctgctgt
     9781 aatactggcc atcggcctcc aaagccatgg cgtgcggaac ggtcaggatg ccaccggcca
     9841 gcaatgtggc ggcagcaacc acgcccgcga tggagccctt aaggccctgc tgcatatggt
     9901 gtgtgttcat gcagcatttt ctatcagccg caagcaaaca agacacacga tggtaaggtt
     9961 acgagtctga acttttgcta tgcgttgtat gaacaatgcg cgacgccccg ccgtaagatg
    10021 acggaatgcc gcgcattgtc aatacaggct gtatcagacg attgcctgtc agtgaatatc
    10081 gtcgggaatg tcgatatcgg ttttctggac ttcttcgacg ttcacgtcct tgaaggtcac
    10141 gatgcgcacg gacttgacga aacggctgga acggtagaca tcccataccc atgcgtcagt
    10201 cagctgcaca tcgaaataca cgtcctgacc agcggaacgc acgttgaaat cgaccttatt
    10261 cgccaagtag aagcgacgct cagtctccac cacataggtg aacagcttga tgacgtcacg
    10321 gtattcccta tacagcgcga gttcggcgtc ggtttcgtaa ttgtcgagat cctcggcact
    10381 catgcttgtt tactcactca ctttccgtat cgcgtttgcg gccgcggccc agcgtacgcc
    10441 accatgcttc ctttgagggc ttgcgttctg cctgaccgta cggccagttg ctttcctctt
    10501 cgagactgtg gcgcgtgttc ttctgcgtat cgtcgtggtg ctcggcctgt ggctcgactg
    10561 gctgtgcggc cgccggcgtc tgggcctggg actgcacgtc actcgtcgtg gcgccggctg
    10621 cctcaccacg ctgtgccggc tcgggttccg gcttgttctg ctggatggcg atggaatgct
    10681 cgcgcgcgga cagcgcttcc ttgacacccg ggttgtcctc aatcaagtgc atgccaaaag
    10741 cgtcaagcga acgaatcgga tcgtactcgt cagccaacgt gtccatgacg ataaggtcct
    10801 ggtatttgtt gttttcggca tcccacaacg cgtcgcgcga ggtgccggac ggcacgaagc
    10861 cgagcgactg gtagaccgaa atcgagcggc tgttcttttc cggcacgctc acccagatgc
    10921 ggtgcatgcc caagccggcc ggttcgggcg cgaaaccgta ggtcatcaca cgcggcatgg
    10981 cgtcacggga gtagccgcgg ccacggtact cacggcccaa gatgatctgg atgcgggcgg
    11041 acttggacca gccgtcgatg tcgataagga aaatcatgcc gatgacatta tcggtgctgg
    11101 ccgcatcgag ctgaccgtca tcgtcatggt cggcgtcagt caatacggcc catgcgatgg
    11161 tgcggcggga ttccggatca cccacaccgg attcggcggg tgcctgaccg gcttcccaag
    11221 cctgggagcg gcggacccat gtgtgtacga tggcgcgttc ggtgaccgca tccttacctg
    11281 tgatcttgga tgcgccatag aaggcatcaa gttcatccaa acgagccagg tcctcgacgg
    11341 tggcggggcg caggtgcacc atttcgccac ggattggcgg aatgacgatg ctctgtggca
    11401 gatcgcgctt cagttgcgct tcggtagcca tgctcagcgt ctcgttggtt gcggtcatcg
    11461 gtatacgtcc cccggttcgg cttgtttcgg ctcacggtcc agtgccgaac atatcttact
    11521 cgactgggtg aacgctggaa atgctctgag tcgactttct tacagaatca ataaaggctc
    11581 ccgctggcgg gagctgtcta acggatttga actggagaat ttccctacaa ataagtgctc
    11641 ccgctggcgg gnnnnnnnnn ngactgaggg tggtcatccg tacatggcga ccaccctcag
    11701 tctcactccg ttcgacagct cccaccagcg ggagccagtg aatctaatca gcccttgatc
    11761 ttgcccatga cgaccttggt gacggccttg gcgtcggcct gaccgcgcgt ggccttcatc
    11821 acggcaccga tgattacacc catcggcttc atgttgccgc tcttgagctt ctcgacaacg
    11881 tccgggttgg cttcgaacgc cgcgtcgacg gccgcctcga tggcaccgtt gtcttcgacg
    11941 atcttgtagt cgtgcttctt gacgacttca tccggagtgc cttcgccctt aagcacgcct
    12001 tcgacggtct gcttggcgag cttgtcgttg agcttgccgg aggcgatgag cttctcgacc
    12061 tcggccacat cggccggggt gatcggcagc tcttcgaggg acacaccctt ggcgttggct
    12121 tcgcgggaaa gctcgcccag ccaccacttg cgggcaccgg cagccttggc accggccttg
    12181 acggtttcct caatcaggtc gagggcgtcg gcgttgagga tgtcgcgcat ctgcagatca
    12241 ctcaggcccc actcggactt gaggcggttg cggcgctcgc gcggcatttc cggcatctgg
    12301 gccttcatct cctcgatgtg ctccttggtg atgtggagca tgacgaggtc ggggtccggg
    12361 aagtagcggt agtcgtcggc atcggacttc agacggccgc cagcggtggt ctgcgtagcc
    12421 tcatcccagt ggcgggtctc ctgcagaatc tccttgccgt cgtccagacg agcggcctga
    12481 cggcgaatct cgtactggat ggtcttctcg atgccgcgga acgagttcac attcttggtc
    12541 tcggaacggg tgccgtacgg agcgtccggg ctcgggcgca acgagacgtt cacgtcagcg
    12601 cgcatgttgc cctgctccat acgagcgtgg gagatgttca acgcgcgcac gatgtcgcgg
    12661 atggcgcgca cataggcacc ggcgatttcg ggagcgcggt cgcccgcccc ctcgatnnnn
    12721 nnnnnnccgg cacggttgta gtcgaccagc gagtggtcgg cgccttcgat acggccgtcg
    12781 gcgccaccca cgtgggtgtt cttgccggcg tcgtcctcga tgtgcgcgcg ctcgatcggc
    12841 acgcggaaca cggtgccgtc ttccagctcg acgtccaggt agccgttgcc gttggtgggc
    12901 ttgtcgtact gcgagatctg gtagtcgcgc ggcatgtccg ggtagaagta gttcttacgg
    12961 gcgaactggc tccactcggc gatttcgcag tgcagggcca gacccagctt gatggcgtaa
    13021 tcgacggcgg tcttgttgat caccggcaga gagcccggca ggccaaggct caccggggtc
    13081 agctcggtgt tcggctcgcc accgaactcg atgtgggccg ggcagaacag cttggtgttc
    13141 gtggacagct ccacgtgggt ttccagaccg aagaccacat cgtacttctt ggtggcatcg
    13201 gcgtacttca tcagtttttc agccatgatg ttgtttcttt ccttccgatg ctcacttgcc
    13261 cagaccgtca agccacgggg tcttgaggtc gttccagatc gggccgttcc agctgtcttc
    13321 cagcgcggct tcgagggccg cggccggctt gtacatgact tcgtcgcgct gctgcggggc
    13381 gatgaactgg aagccgacgg gcaggccgtc gtcggacagg ccggccggga tggacatggc
    13441 aggcacgccg gcgaggttgg ccggaatggt ggcgatgtcg ttcatgtaca tggccagcgg
    13501 gtcgtccatc ttctcgccga acttgaaggc ggtggacggg ctggtggggg ccacgagcac
    13561 gtcgacctgt tcgaaggcct tcttgaagtc ttcgataatc agcgtgcgga ccttctgggc
    13621 cgagccgtac caggcgtcgt agtagccggc ggacagggcg taggtgccga ggatgatacg
    13681 gcgcttgacc tcgtcgccga agccggcttc acgggtgtag gc
//
LOCUS       DAWBMW010000025        13442 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_1172_length_13442_cov_3.014193, whole genome shotgun sequence.
ACCESSION   DAWBMW010000025 DAWBMW010000000
VERSION     DAWBMW010000025.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 13442)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13442)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13442
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1172_length_13442_cov_3.014193"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2534 a         3968 c         4088 g         2852 t
ORIGIN      
        1 ggatcgtgga cgagtccaag gttgtggaca ccatcggcaa gttcccgctg ccggtcgagg
       61 tcattccgtt cggtgccggc caagtcatca agaagttcga ggcccgcggc tacaagccgg
      121 tgcttcgtct tgacgccgac ggcaaggaag tgcgcaccga cgagaacaac ttcgtggtgg
      181 acctgcacct ggagcgcatc gatcacccgc aggagctggc cgaagacctc atcaacacgg
      241 tcggcgtggt ggagcacggc ctgttcctca acatggtcga caaggtcatc gtcggcgacc
      301 cgaacggccc gcgcgtgatg accaacgcca acaagtgatc gtctcacatc actaagcttt
      361 tccaagcccg catctgcaca tcgcaggtgc gggctttact gttgcccgaa caaggcaaag
      421 agccaagcaa aagagaaacc ccgcgaccat aacgatcacg gggtttctaa tgccttgatt
      481 gcaggtaaga ctacttacgc tggtggcggg tcttacgcag cagtttgcgg tgcttcttct
      541 tgctcatccg cttgcggcgc ttcttgatga cagaacccat ctgaagcctc catttcgatt
      601 aatcgtaaaa gttgcaactc gcaccataat acacgtgttg ccagacagag gaaaccagta
      661 tgttgtcgat agtccgcata tgccctgaac gcagttaggg catccacatc agcaatgtgg
      721 atgccccaac gttcataagt ttgtgcagca gctgacagcc gatactagat actacatcgg
      781 gaacttctgg tagaagcgat cgaccgcttc cttggtgccg gtagcttgga agaccacggt
      841 atcgttctgc cagatggcaa cggcgttctt gctgttggag gcatcggcct tcaccacgta
      901 cgcgcccgtg acgttgccgg aaaccttgac gttgccggat gccgactcat cgccggtctg
      961 cgcagcgacc aacgcatcgt actgggtctt ggcattgtcc gccgacgacc actgcgcggc
     1021 gaccagcgtg acatccttgg cggcatcacc agtggagtag gtcagagtgt actcctcgag
     1081 cggcgacgcg gaattccacg tcgtggaggc atccgccttg gtacgcgcgt aattcagcac
     1141 gctgcccggc atggccttca acagctcact ggcatcatcc ggcaaagctg tggcgtcgat
     1201 ggtcggcttg gtcggctcgg atgattcggt tttctgctgg ctgttttggg tggtagtgga
     1261 ggtgccatca ttcttctgga tggcccaacc cggccacaca aaggccgaaa gcactgccag
     1321 gataatcaca acagctgcgg caaccaccac tgcaatcaac ttgccatggg attgcgtggt
     1381 cgaggagttc tcactattag tcatgccccg attctctcac agccgcgtga cgctaggcgg
     1441 ccgtaatttt agtcgttgtg cgcaagccgc gtgaaactct tgtgaacggt aaaaatttac
     1501 cgtccgctgg tgtttgggtt agggtgagac catggcgaaa tcaagtgtgc agtatgtatg
     1561 ttccgaatgc ggttggaacg ggccgaagtg gtacggccgt tgccccgagt gtggtcagtg
     1621 gggcacggtc gaggaatttc acgaggctcg gccggcggca ggctctcgca cggccgctcc
     1681 cggccgaaca tcacgcacgc agtcggttac ggttgccaac aacgccagat ctgcagcccg
     1741 tccgattacc gaagtgggca cggaaagcgt cgagcggctc ggtaccgggt tttccgaatt
     1801 cgaccgtgtg ctcggcggtg gcgtggtgcc gggttcagta acgctgatag cgggcgaacc
     1861 gggtatcggc aagtcgacat tgctgttgca gacggccggc aatatcgcgc gcgttgtggc
     1921 gggagacagt acgttccgag gggcaggtca gcggtctgcc caacagtcgg gccgggcgca
     1981 gggcaacgca tcgaacacgg tgccgatgcg gggcaacgca tcatcaacac acgccaacac
     2041 cgtgctgtac atttccggcg aggaatcaca ggcgcaggtt cgtttgcgcg cttcccgcat
     2101 caatgcggtg gagccgaact tgttactggc ctccaccacc gacctgtcca cagtattggg
     2161 gctgatcgaa cagaacaagc cggccttggc cattgtggat tcggcgcaga ccatcgtctc
     2221 gcaggaggtc gatggcattt ccggcggttc gactcaggtg cgcgaagtgg caagcgcgct
     2281 gatagatacc gctaagacac ttgacattcc agtatttctg gttggtcatg tgaccaagga
     2341 tggttcgatt gcaggcccgc gcacattgga gcatctggtc gatgtggtct gccaattcga
     2401 aggcgacagc gagactgcgc tgagaatgct gcgagcggtg aagaatcgtt ttggcccgac
     2461 cgatgaagtc ggctgtttcg acatgagcgg tgaaggcatc gaagaggtca ccgatcccgc
     2521 cggattgttc ctgtctggag acggccctga tgcggccaac gcggcacccg tggaaggcac
     2581 ctgcgtgacg ttcacattgg atgggcatcg cagtctgcct atcgaagtac aggctctggt
     2641 caccacgtcg gtgctgccca caccccgtcg tgcggtcaat ggcgtggacc ccagccgtat
     2701 cgccatgctg gtcgcggtgc tctaccgtca cagcaagctc aacctgctgt ccaacgacct
     2761 gtatatctcc actattgccg ggggccaggc caaggagccc ggttccgact tggcgatagt
     2821 ggcggcactg gcgagcgccg ccacgtcaaa acccattgcg cgagccacgt gcgccattgg
     2881 tgagatttcc ctgaccggtc aggtgcgccc ggtaccgcgt atggaatatc gccttcgcga
     2941 ggccgcacgc ctcggcttca ccacggcggt cattccgcca ctgcgcaagc cggtgcatgt
     3001 cgagggactt cagctggtgg agtccaacac attgtccgac gcattagacg ccttgggagt
     3061 gcgtaaatag ccctgagata tgcctcaggc ggtaagacgc ttggtctcct cgacgttgcg
     3121 cttgagttcg tcgacgagtt ggtcggggcc atcaaacttg acctgcgggc gcaggaagcc
     3181 gacgaattcg acgcgaacct tgtggtcgta caggtcgatc cattcttcag tgatgcagta
     3241 ggcctccacc acacgctcat taaggccggt tttttcactg aaggtgggtt tggtgccaat
     3301 ggagatggcg gcgggccaac ggttttcacc atctaccagc caaccggcat agacgccgtc
     3361 gaccggaatg tagcctgcaa tgtcggagcc aagattggcg ggggggaatc cgatggtgcg
     3421 gccacgctcg tcaccatgca ccacttcgcc ctcgatggag tgcggagcac ccagaatggc
     3481 gttggcgtcc tggattcggc catgagccag caggtagcgc acattggtgg agctccatgc
     3541 gcgcattgcc ttggcctggt tcttcttgct ccatgcgcgg cgctcggctt tggtggcatt
     3601 ggccagcgga tcgacgggct ctccccattc gctcggggct tccggcttga aatcgcgcgg
     3661 aatgcgcact tcacccgggc cacggtcatc gaccacgtca agttcgaata caccggtggc
     3721 ggcggcaaga ttggcgatat gcttcacatc accttctcga cccttgccca tggcggcatc
     3781 ctgcccaagg accaacgtgc gcatacccag cttgccggtg agtcggccaa ggaagaaaat
     3841 gtatgacata gctgcgaagg ccagcgtgta gtgcaccacc agcacatggt ccacgccgaa
     3901 ctgttccatg cggtgcagtc gatcatccac actggtcaat gcttcggcat ccacgttctg
     3961 aaccgcgggc tcttcgccat ggtgcgagat gacccagccg tgcacgaatg cggggcgcgg
     4021 gtcaaacaga atcaccacgg aaaaagcatc atgctggtgc gcgagttcca ccacacgctt
     4081 gatcaccgcc tgatgaccca agtgcatgcc atcaaatgcg cccaatgtca ccacggcctt
     4141 cttatcgtta ctgaacgagg gccattcaat cgaaccttcg gcgtcagggg tcaggtgcgt
     4201 gatatccatc agtgtgctgt tccttttatc cgtgcgatgt tattcatgca atatagacgt
     4261 tgttgtgcgc ggcctgtatc aagctgcact tttcaccgcg tctactttac gcgaggggga
     4321 atacggtgac cggcttggcc tgatgagcgt ttgcacgctc gataatcgcc gccacatcat
     4381 gcgtttgcgg cacaatggcg gcagtgggct cgctgattgt acgcacaata cggcggccga
     4441 agcgtaattc gctggcctcc tcaggagtga tgtcaagcgc cggcatcgca cctcgagcgg
     4501 cctgttccat ggtaagggca tggtcaagca gccagttgcg gcgctcgtca ccggcaagac
     4561 cttcaggagt gtccaatacg cacttgttgc gcgtgacggt ttgcccttcc cgattagtga
     4621 acgtcttctg atcggtatgt gcggtgacgg tatgggttcg cgtgtcaagc atcgcctcag
     4681 gagcgataag tccgctggca tcgtccggca acgcgaatcg gccgactcgc gtacggcgca
     4741 aacgggtcag gtatccgccg acgccaagtt ctttgccgag atcgcgggcg agcgcgcgga
     4801 tataggtgcc cgatgaacag ctgatccgca cgtcaacatc aagtgcggga atcgctcccg
     4861 tgttcgcgtc ttcccgcaat ggttcagtgg cctgctcccc tgctacgaat ccgctgcgga
     4921 tatccagcac ggtgaattcg ctgatggtga ccgggcgggg tttgagttcc acatccttgc
     4981 cttcgcgagc caagtcgtag gcacgctggc cgttgatttt gatggcggag aacgtattgg
     5041 ggacttgttc gatatcgccg gtgaaattgg tggcgatggt gcgtgtgagc aggtcttgcc
     5101 atgaggcagt tggtaacgcc gttggttcgc cgctctggcc accctcagtc agctgcgctg
     5161 atagcgtttg ccagcgggaa cgggcttcac catcgacaag cacctcgcct tcggcatcgt
     5221 cggtagtggt gcgcaggccg aggcgtatgg tggcctcgta ggttttgtca tgcgccacga
     5281 tggcgttgag cagtcgtgtg gcgtggccga aggcgatgac cagcgtgcca gtggccatgg
     5341 ggtcaagggt gccggcatga ccgaccttct tgatgtgcaa cgcgccgcgc acggcggcga
     5401 cggcgtcgaa gctggtcacg ccttgcggct tgtcgatgat gagcagaccg ctgggtgttg
     5461 tatgaagcac actactcctc gtcagagtcg tcttcgaagt cgtcgctggg ctcgtcgtca
     5521 tgcttgtacg gatcggcgtc gccggcgtac tgggcggtgg cgcgagcacg ggcgagttct
     5581 tcatcacgct tcttggccac ggcgagtatg tcttcgatct cgtgagcctc gccgggcact
     5641 tcatcgaaca cgaattggag ctgcggagtc aggcgcaggc cagccttgtg gcccaccaga
     5701 gaacgcaggt ggcccttggc ctgatcgagt gcctgctggg cgcgcttgcg ttcgccttcc
     5761 tcgtggcctt cgtggccgag ctgggtccag tagaccttgg cgatctgcaa atcgttggtc
     5821 acacgaactt cggtgacggt gatgctggcg agtcgcttgt catgcagttc gcgctcaatg
     5881 gaggaggcga cgacacgctg aatcaaggcc gcaatacgtg cggcgcgcgg gttggttcct
     5941 gccataggaa tttccttttc tagacttgag ccccctctga cgaggggctg ccgaacgcag
     6001 tgaggctggg ggagagaaaa tcatggaact accatgtctc tcccccagtc agtctgcggc
     6061 tgacagcccc ctcgtcagag ggggcttaac aatgactact tgcgctcgac ttcccgcatc
     6121 tcgaaggtct cgatgatgtc gccgagttca atgtcgttga aggtgccgag gttgatacca
     6181 gcctcgtagc cttccttgac ggactgaacg tcgtccttga agcgacgcag cgaggagatc
     6241 tcgaggtcgt tgaccgtggc cacgccgttg cgcaggatac ggcacttcgt accacgcttg
     6301 acctcgccgt cctgcaccat aacgccggcg atgttgccga acttggagga gcggaagatc
     6361 tcgcggatct cggagtgaga ggtgacgacc tcttcgtatt ccggcttgag catgcccttg
     6421 agggatgcct cgatgtcttc gatcgcacgg tagatgaccg agtagtactt gatctccacg
     6481 ccctcgcgct cggcgaggtc cgcgacctga cggttcggac gcacgttgaa accgatgata
     6541 acggccttgt cgaccgtggc caggttgaca tcattctggg tgatcgcacc gacgccgcgg
     6601 tggatgacct ggatgccgac ctcgtcggat acctcgatct tcatcaagga atcctcgagc
     6661 gcctcgacag aaccggacga atcgcccttg atgacaatat tgagcatgtc gatctcggac
     6721 tcggcgaact tcttcttgaa gtcctcgagg gagacgacct tgcgacgctt ggccagctgg
     6781 gcggcacgct cggtggcctg acgcttctcg gcaatctggc gagcggcacg atcatcggag
     6841 gcgaccagga agaggtcacc ggcggtgggc acggaggtca agcccagcac ctgaacaggt
     6901 gtggacggac cggccgcttc catcggctga ccgttctcgt cgagcatggc acgcacgcgg
     6961 ccgtacgaag tgccggccac gatggcatcg cccacgtgga gcgtaccctg ctgaaccagc
     7021 acagtggcca cggcaccacg acccttgtcg agtcgggctt cgacggtggc accacgggcg
     7081 tccatgtcgg ggttggcgcg gaggtccagc tcagcgtcag ccgtgagcag cacggcttcg
     7141 agcagcttgt cgacgtgcag gttctgcttg gcggagatgt cgacgaacat ggtgtcgcca
     7201 ccatactctt cagggaccaa gccgaactcg gtgagctggc cgcggacctt ctccgggttg
     7261 gcaccgggca catcgatctt gttgacggcc acgacgatcg gcaccttggc ggcctgagcg
     7321 tggttgatgg cctcaacggt ctggggcatc acaccatcgt cggcggcgac cacgaggatc
     7381 gcgacgtcag tgagctcggc accacgggca cgcatggcgg tgaacgcctc gtggccaggg
     7441 gtatcgagga aggtgatctt gcgcggttcg ccttcaaggt tcacggtgac ctggtaggca
     7501 ccgatgcgct gggtaatgcc gccggcctca cgtgcgatga cgttggtctt gcggatggta
     7561 tccagcagtc gtgtcttacc gtggtcgacg tggcccatga cggtaacgac cggcgggcga
     7621 ggcttgagat cctcgtcatc ctgcagttct tcctcgtcaa ggttgatgtc gaactgctgg
     7681 agcagttcct tgtcttcctc ttctgcggag acgagctgga tgttccagcc gatctcttcg
     7741 cccagaatct ggaaggtctc ctcatccaag gactgggtgg cggtagccat ctgacccagg
     7801 tggaagagca cggtgaccag cgcggcctgg ttgacgttga tcttctccgc caaatcggcc
     7861 agggacgcgc cctgacgcag acggatggtc tggccgttgc cgttggggat gcgaacgccg
     7921 ccgatggtcg gtgctttcag ctcctcatac tcatggcgct ttgccagacg gttcttacgg
     7981 gcctttgagg acttgccgcc ctgacgtccg aatgcgcctg cggcaccgcc gcgaccgccg
     8041 cgaccgccgc cacgtgcggg accgttgctc ggtgcaccgc caccctggaa gttattgccg
     8101 ccgccttggc ccccacggaa gccgccgcgt gcgccgccct gaccggcacc ctgggagccg
     8161 ccgccctgac ccggacggtt gtggccccac tggccgggac gcggaccgcc ctgaccacct
     8221 tggcccggac gaccgccacg gaagccgcgg ccctgcccct ggccttggcc gggacggccc
     8281 ggacgaacgc cacgaccgtt atcagcagtc ggacgagcca ttggatgcgg acgcggaata
     8341 tcacccggcg tcggggtgtg cataccctgc ttgcgggaga acgggttgtt gcccgggcgc
     8401 gggccctgag cgtgcgggcg cggaatcgca tttgaagcgg cattgccgcc attggcaccg
     8461 gcgccgccct gagcgtgcgg gcgcggagcc gaagcattgc cgcggttgtt ctgatgctga
     8521 accggctggc cattgttcgg acggccctgg ttgttacgac gatcgttgtt acggcgatcg
     8581 ttcgggcgac tctgacgacc gttctcacgg ccttcgcgat tatcgcgacc attctcacgg
     8641 ttgtcgttct gaccgtggcg gccgggggtc ggcatgccgg ggcgtgcgcc gccctgcgga
     8701 cgcggtgtcg gtgcacccgg agtcggagcc tgctgatgtg ccgaggcagg cgatgcctga
     8761 cgtgcgggag cggccggagt cggtgcggaa gatgaagcgg aagccggggc cgaaggtgcg
     8821 gccgggcggc gctgctgcac gggcttggaa tcgcccttgg cgttgtcctt ggcgaactct
     8881 gccttcagac gccgggctac cggcggttca atcgtagaag aagcggactt gacgaattct
     8941 cccatgtcct taagcttctc aagcacggtt ttgctatcga cattcaaaac cttagccagt
     9001 tcgtatacgc gtggtttcgc cactaattac tcctattctg tgaccacgcg cgtgcatggg
     9061 cgtggtcagt tggtgatgac ggcaagccta ccctgtctca tcgtgcgacc atgatcgtgt
     9121 tacctcgtct ctggtgtggg cgatttcgcg cacactgcgc cagcccacat acgccaatca
     9181 agcatactca tagcgctgga tagcgtagac gcagcgagcc gcgccattgc tgacgcggct
     9241 cgtatcagga aataatgcgc cctgaagatc attcgccttc gagcccggcg gcctattcgg
     9301 cttccggagc agcagcctcg gccgcagcct gggcggcctt ttcggcggcg actttcttgg
     9361 catgggcctc ggcggattcg atgccgatct tccagccggt cagcttggct gccagtcgtg
     9421 cgttctggcc ttccttgccg atggccaagg acagctggtc gtcgtggatg aaggcgatgg
     9481 cggtcttgtt cttctcgctg atcacctgca cgccggtggc cacagccggg gacagcgcgg
     9541 ctgccacgaa cttggccgga tcgtcggagt agtcgacgat gtcgatcttc tccgggccaa
     9601 ggttctccat aacggcacgc acgcgagcgc cgccggggcc gatcagcgcg cccttcgggt
     9661 tcacgccatc ggtgttggcc ttgaccgcga tcttggtgcg ggcgccggcc tcacgagcga
     9721 tggccatgat ggacacggca ccggagacca gttccggcac ttcgcgctcg aacagcttgc
     9781 ggaccagctc cgggtgggag cgggacacca cgatttccgg gcccttgatg ccacgggcca
     9841 cgttcaccac gtacacgcgg atgcgttcgc cgtggcggta acgttcgccg ggcacctgct
     9901 cacggcgagg cagaatggct tcgacatcgc ccatcgcgac atgcacgttg gaggggtcgg
     9961 aagcatcctg ctggatgatg ccggtgatga gcttgccctt ctggccggag aacgcaccga
    10021 ataccttctc atcttcggcg cggcggaaca gctgggtgat gacctggcgt gcggtggctg
    10081 cggcgagacg gccgaagtca cgcggggtgt catcgtattc ctcgcccagc ttgggaacgg
    10141 ggtaagggtt gtcttcggtg ggttcgaccg gaatttcgtc ggcggcccac acggtgaagt
    10201 tgccggaacg ttcgtcaagc tccacgcgcg cgtgcttggc ggcgtgcggg gtcttgaggt
    10261 aggcgaggcg cagggcctct gccagcgcat catccagggt ttcggggtcg attccttgct
    10321 cggcggcgag cttgtgtatt cctgtcaggt ccagttccat cgatccgtac ctcctatgaa
    10381 gttatgtggc gcatggccta tgagtagtca cagttagcta ttagtgtatg tttccatgca
    10441 gacacgcttc tcttcaggac ggtttacccg gcgcttggca tccgtactat gcgcgagcgc
    10501 gatgctgtgt tcgctttcgg cgtgttcgat gccgcgattg gccggacggg ccgaggcgga
    10561 agctcaaatg accccttgcg agaaagcgat gacgttggcg ggctacgcca cccatccggc
    10621 tgaaggcaca ccgttattag agcagtatgc caccggtctg gccgcccccc tggcttggat
    10681 tgacgttgcc gggtattgtt cgggtcgttt tgccgaggga acgttacgtg acgcgcagac
    10741 caagcaatgg atggcttttc tggccgataa attcgggcag tccgcgcctg aggtgacgcc
    10801 ggcgcgtctt gatggcgtca cgtccgccaa cgttgaccga tcggtattgg atgcgatggc
    10861 cgtggccgag gatcgcgccg gattcgctat cgaggtcttg gccgcacgag gacagaccgc
    10921 tggggctacg ttggcgttga gcgatatgca taagaccgcc gggcagcagt tggttgcgct
    10981 ggcgaacggc aattttgatg attccggcgc acaatccagt tcttccggcc aaaacgaccc
    11041 ccgccaaaag gtctatgcca tcgaccaact actggccaac cccaccacca tcgccgacaa
    11101 ggcaagcggc caaaccgtac cgaccgccgc cgccatcgaa atggactgcg cccgcgcgca
    11161 gatcaaggcc gtgaccgaat cgaaatcttc caccgagagc gatacgctgc tcatcctcgc
    11221 cgcactggcc gccaagcacg cctacaccgc gttccaactt ggctacccag cgactgacgc
    11281 cgcattgttc gagtagacac acactcctca cgaagccgat gcgaacttgt tggatgctag
    11341 tgggcaagtc gagttgaggc acgttccacc aatgtgaaat cggtggtgta cgtacgggcc
    11401 gggcctgagt atccttcgat gcggtcgata agcatgtcaa cggcatgctt ggcataatca
    11461 tcgatatgag gatcgatggt ggtcagcgcc ggcaccgagt actcggcttc cggcacgtta
    11521 tcgaagccga ccacttgtac atcgtcaggt acatgcaaac cacatcgttg caattcatga
    11581 attgcgccta aagcaatggc atcattgaga cacactacaa catccgggca tgggagcttg
    11641 tctagcatag cttttgtcac gcgcatacct tcacgctgat tcatgctttc tgccggaatc
    11701 acataccgcc aattcaccga taatccgcgc tcttcaaatg ccttcacata tccttgggcg
    11761 cgtaacgcct gggccccttc cgtctgacga cgcagcactg cctcatccac cagttcagga
    11821 agaacaccca tcagagcaac cgtttgggca ccttcatcca aaaatcttcc tacaaccgtg
    11881 gagatggatt caatgttggg catggtgatc caatcgcttt tcccgtaggc cagatggtct
    11941 ccggtcgtta caatcggata tggctgctcc agaacggccc cttcatccgc gagcggcgac
    12001 attacgaaca gaatccatcc ttccgcccct aaacgatagg actcgtcaac gctggcaacc
    12061 aacccttcac cgtcggaacc atatgtgttg atgactacac cgtaatggcg ttcttttgcg
    12121 tattcaatga ccttgtcagt gaaaaaagga acgaacggct gcgagaaatc gaagatgttg
    12181 agtccaataa gctttgtgcc gccagatcgc attgcacgcg ccgaaatatt gacggtatat
    12241 ccaagcttac gcatcacctc ttccacacgg cgacgtgtat ccggtctcat actgccagtg
    12301 tcattcagca cattggaaac cgtcttgatg gagacgttcg cctcacgcgc cacgtccttg
    12361 atagttacgc ggtttcccat tgttcgaatc catttctgct tattacatga cacaccttca
    12421 acagcgtcat tatgttctta ttatggtaag tcacagtcgg gtcgagcatc tcctcctcat
    12481 atcagaatca tctacccctc aacctcagaa ttcagccaag gagctttcct ccgttggacc
    12541 ccatgcacca ttaggcaaat atccttctga caatactgca ggacgatcga acgtagaggt
    12601 taaactttct ggttgaagaa acgcacgcac gccatccgaa caagggactt tctgaccgcc
    12661 ggctttcaac ctagcttttt acatacccgt cttttctcct tattcctttg ttgggtatcg
    12721 aattgttcca gtcgatgtga atgacggcat tgtcttcctt tgactggtat gccacattac
    12781 attagtggcg aatccgaacc tctctttctg caatccgggc atgttgatga ttgtttgggt
    12841 attgcttggt ggttgtacag ggcacagcaa ccataagaga ctgcgccctg tcatgcgcca
    12901 ccgtcactcg aatcacttca cgtgcacggc ggtccaggag accggcggca aggtcacacg
    12961 cacagtgccg gtatcggcgt cggacgtgat ggtggtgttg gggtgcagaa cgactcggtt
    13021 ctggtcttcc agcgtgtttt ttgccagcag atcatcctcg tgcagggttt gcgcctcgac
    13081 cgcggaaagt gcgaatcctt ccggcagctt gatggcaaag tcattggccg cttccatgga
    13141 gcgattgacc acaaacacgc tgatcgtacc gtcctcacca cgcacggcta cggaattgat
    13201 cgtcggcact tcaccgtatt tgtcggtttc atacgttccg gaagccagtt tcggttcaag
    13261 caccgtgccg cccttggcga gctttgcggt cagcgagaac ggatagaacg tggtctgacg
    13321 ccacgccggg ccgcccggtt cggtcataat cggcgcaatt acgttgacca actgggccag
    13381 gctggcggcg tgcacgcggt cggcgttctt gagcagggta atcatcaaat caccgaacac
    13441 ca
//
LOCUS       DAWBMW010000026        13071 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_1217_length_13071_cov_2.473110, whole genome shotgun sequence.
ACCESSION   DAWBMW010000026 DAWBMW010000000
VERSION     DAWBMW010000026.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 13071)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13071)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13071
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1217_length_13071_cov_2.473110"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    8232..8241
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9695..9704
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2445 a         4146 c         4036 g         2424 t
ORIGIN      
        1 atggcgacaa gggcgatttc gccccagacg ctttgttcgc cgtcctggcc ttcgcgcgtg
       61 tagtgcacgg tggcaccaag gttcgcgccg gtgtccacat gggtgacgct ggcgcgggtg
      121 atgatgttca cgccatgacg tttgagctca cgggtcagcg tggtgccggc tctccggtcc
      181 caggctgaga gcacgcggtc cttgcgaatc agtagggtca ccttcgaacc ggcggcgttc
      241 cacatcgagg cgaattcaag ggcgatggcc ccggcgccga taatcaccgc cgacgaggga
      301 aattcgttga cttccagcgc ctgtgtggag tcgatgaggg cgccggcgaa cgggttgccg
      361 ggcaatgggc gtggcttggc accggtggcg atgacgatgt tcgtggccgt gaggtccatc
      421 gtggggccgc tgggctcggg gacatcggcc ttgtgatagg tgaggatgtc gctttggtcg
      481 ggggagggaa cgaggtggac gatgtggttg gaagttgctg gagcggtctc atccgcatgg
      541 aacgtggcat tggctcggaa cacggtgatg ccgcgatggg caagcagtcc ggccagtcct
      601 cccaccatcg tcttgaccac gcgcaggcga tagtcgcgca gcgtgccgaa gtcgatgcca
      661 ttcaccgagg cgttgacgcc gagttcggcc gcacggtgca cggtgtcgat ggtgtgggtg
      721 gcggtgatga gcgccttgga ggggatgcag ccacggttga gacatgtgcc gcctacggtg
      781 gcgtcacgct cgacgagtgc cactttcatg ccgagttcgg cggcgcgcag ggctgtggaa
      841 tagccgccgg ggccggcccc gatgatgacc agatcaaact gttcgctcat gattgcctcc
      901 ttgatacata taagtatggc tcccacgcta gggagccata tgcggctatc gccgccactt
      961 cgcctaaaaa cggtccactg gactgttttc tttacggctc agcccctcaa cgaggggagc
     1021 cagagtatat atggacaatc agtccgccgg ccagtgaccg cgcttcgggt aacgcggctt
     1081 gggaagcctc caacgacgca tctgaatcga acggctccaa gcgtaggaga aggagcggga
     1141 gcccttggac gcggactgtt cgccgtactt ggcgatgagc ttggccttga gcttgcgcca
     1201 catcagccat acgtcgatga tcacggcaat caggtaaccg tacataagcg ccatcatgat
     1261 gatggaagcc cactggttct gcatcaaaga agtgacgaac atggagacga tcagaatcgc
     1321 gaaggccacc gggatgaacc attcgccaag gttgaaacgg gcatccacat aatcacggat
     1381 gtagatacgc caaggcaggc gttccgcctt gggcatgtgg ttgatatcgc cattctgcat
     1441 cgcctcgtac tcggcgttct ccttctcacg cagacgggct ttggcggcct tggcgctggc
     1501 cttgcggtcc accggaacca gcggacggat accggcggcc tgagcctgct tcatcttcgg
     1561 ggtaggacgg ccttttttct cagaaggatt ggcggccttc ttcggggcct gctcaacagg
     1621 agcctggccc tcttcttttt tcttaaacgg attccatgtc atgctttgag gttacggtga
     1681 cgttcagaca cttgtacact ggcgcagcca ttgcaatgac gccgctcaca catatatata
     1741 aaggaggacc atcatgacaa cgctcaccgc tgacgagatt cgttcgcgcg tcgaaaccga
     1801 ttggaaccgc atcgtcaagg tgctggccga aaaggtcgcg ctgcagtcga tctccgccaa
     1861 gggcatcacc gccgaacata tgaagcgctc cgctgaattc gtggcagacg aactgcgcct
     1921 cgtgggtgtg gatgccaagg tggtccaggc ttccaatgcc gacggcactc cgggcgcatg
     1981 ggaggtcatc ggctcgcata tcgtttctcc ggacgccccc accgtgctgc tctacgccca
     2041 tcacgatgtg cagcctgttc cggacccggc cgagtggaac accgacccgt tcgtggccac
     2101 cgaaatcgac ggccgtcttt acggccgtgg ctccgccgat gacggcggcg gcatcgccat
     2161 ccactccggt gcgctgaagg ccctgggcga cgacctcaac gtcaatatca aagtgttcat
     2221 cgaaggcgaa gaggagatgg gctcgccaag cttcattccc ttcatcgagg ctcaccgcga
     2281 cgaattcgcc gctgacgtga ttatcgtggc cgattccggc aactggtcgg cggacattcc
     2341 ttctttgacc acctcgctgc gcggcaacac ctgtgtggac gtgaccgtgc agggccttga
     2401 gcatccggtg cactccggcc agtacggcgg cccgattctc gactccaata cgttggccgc
     2461 catgctcatc gcttccatgt atgacgagaa cggcgatctg gccgtacccg gcgtgaccgc
     2521 ggaagagccg atcggcggtt tgcagcacga ccttgacgaa accaccgtgc gcgaggattc
     2581 tggcgtggtg gccggttacg agttcgccgg caccggctcg ctggcctcac gcctgtggac
     2641 taagccgtcc gtgaccgtga tcggcttcga cgcccacccg gtcgagggat ccttcaacgt
     2701 gatcgccccg gagacccgct tccgtctgtc gctgcgcacc gcgcccaccc agcgccctga
     2761 agaagcgcag gaagcactgg ctgcattcct cgaatcccac gcgccgttcg gtgccaaggt
     2821 cactgtcgaa cgcggcgaaa acggcatggg ctgggccatg gatccgaccg ccgtggccac
     2881 caaggacgcg ctggaagcca tgaccgaggc cttcggcgtt gagcccatca acaagggcga
     2941 aggcggctcc attccgttca tccccgaact gcagcgcatt ttccccaacg ctcaggtgct
     3001 ggtcaccggt cccgaagacc cgaaggccaa cgcccacagc cccaatgagt ccatctccct
     3061 gcccggcctc aagaacaacg tgatcaccga ggctttgctg ctggacaagc tcggcaagta
     3121 gccgaaccgc atcggtaatg gtagtgagcc gccgtcaggt aggtcacaga tgtggctatt
     3181 gcctttgacg gcggcttgct ttatagtcgg gagttgacac gggagctgcc gttgggcggc
     3241 tgagaggaga ctcatagtct cgaccgatgg aacgtgaccg gataatgccg gcgcacggaa
     3301 atgtcgctct tacccacccg tgcctgaacc aacccgcgcg tggacacgaa catgtgcatt
     3361 atgtgcgtac atatatccat gtatgttcgg cctgcgtgcg agcagaagaa aggcacattc
     3421 atgactgaag ctgtggccaa agccaattac aagtggcgcg tcgtcgatat cgtcgtcgcc
     3481 gcaatcatcg ccgtcgcatc cggcgtcatt ttctggggct gggacattgt ctgctccgcc
     3541 ccgctggccc tgtttgaagg cgtgaccccc ggtttcgagg gtctgctcaa cggattctgg
     3601 ctgttcgccg gcccgctcgc tgcaatcatt gtgcgcaagc ccggtgccgc actgtttgcc
     3661 gaaaccctcg ccgccttcct tgaacttacg ctgggcaacc agtggggcgt cggcggctcg
     3721 ctgatcgtcg gcatcatcca gggtctgggt gccgaaattg ccttcgccgt cttcgcttgg
     3781 aagaagtgga acatcggcac caccatcctg tccggtgcgc tcgccggcgt gggctgctgg
     3841 gcctactact gggccaccaa cccgggctgg aacggcctgc gcgtcacttg gtatctggtg
     3901 ggctccatca tctccggcgt cgtgatcgcc ggcgtggtgg tctggtactt gtctcgtgcg
     3961 ctcgccgtca ccggtgctct ggagcgtttt gaatccggcc gcagcaaggc gcgcgtctga
     4021 tgcagattaa tccggctgcg gtagaggcca gcggctgggg atggcggcac gccacgcgta
     4081 aggacttcgc gttgcgtgcc gtcgatttca ccatccggcc aggtgaacgt gtgttgctgc
     4141 tcggtgcttc gggcgcgggc aaaagcacga tgatggccgg tctcgcaggc gtacttggcg
     4201 gcgatgagga aggcgagcag gagggctcgc tcaccatcga tggcgtcgat gcgcgcgagg
     4261 cgcgaggtcg cgtgggactg gtgcttcagg acccggactc gcagatcatt cttgaacgac
     4321 tgggcgatga cgcggccttc ggctgcgaaa acctcaacgt gccgcgcgag gagatttggc
     4381 agcgagtgcg cgaatcattg gatatggtgg gactcggcgg actcgaactg gatcggtcca
     4441 cccgccatct gtccggcggt cagcgtcaac gtctcgcgtt ggccggcgtg ctggctatga
     4501 agcccggtct gctgttgttg gacgaaccga ccgccaacct cgatcccgaa ggcgtggtcg
     4561 aagtgcatga cgcggtcaaa aaggtcatcg aggccaccgg acagaccatg gttgtggtcg
     4621 aacatcatat cgatgtatgg ctcgatctgg tcgatcgcgt gattgtgctc ggccgacccg
     4681 atgcaagttc gcccacgggt gcggtcattg ccgacggcaa gccggacgag gtgttcgccg
     4741 agatgggcga cgtgttggcc gccggtggcg cgtgggtgcc gggccgtgcg attcccgatt
     4801 actcaccgaa gcaggagtct gcgcacgatg cgccggtgct cagcaccgag aacttaagct
     4861 ttggccgtgg gaccgctctt ggcgttggta tcaatctgga tttctatccc ggcgaagtaa
     4921 cggctctgat gggtccgaac ggcgcgggca aatcgacctt ggccttgacc ttggccgggc
     4981 tgctcgagcc tatcgccggc aaggtgctca tcgccgacaa cctcaagccc gcgcatgccg
     5041 ggcgtgagcc gattgactgg aaaagcaagg aactgctggg ccgtatcggc atggtgttcc
     5101 aggagcccga acaccagttc gctgccaatt tcgtgcgtga cgaagtcgcc atcggcccca
     5161 agtcgatggg gcagagccaa gacgaggctt atcggaccgc cgaccgcatg cttgagcaga
     5221 tgaatctcac gcgcttcgcc aaagccaacc cgtatacctt gtctggcggg gagaagcggc
     5281 gactgtccgt ggcttccatg ctggccgccg cacccaaagt gttggtgatg gatgagccga
     5341 cattcggaca ggatttcagt acgtggaatg agatggtcaa gctgatcgcc gccatccgcg
     5401 atcaaggctc gtgcgtggtc atggtgacgc acgatgaggc gctggtggat gcgctgggcg
     5461 cacgacgtgt gatgttcgag gaggcgaagg catgagtgac gtggtaaccg tcagtccgca
     5521 ggaaacgcgc gtggagccgg tcaaagcctc atcgccctca tggttcatcg cgcgcatcaa
     5581 cgcggtcagc cgcatcatcg gtgcgctgtt gctgtgcgtt ccgatgttca tcagtctgga
     5641 tgtggtatcc gcgtccacgg cgctcggcat cgaattcgtg ctgctgtgga ttggtggcgt
     5701 ggctccgtgg accgtatgcc gtaagacctg gccggtatgg atcgccgccg ccggcagttt
     5761 tatctccgtg tttctctacg gccgcacctc gggcgagatc ctgtggcagg gtggctggat
     5821 tgtgatcagc gagggatccg ttaatctggc catcgccacg gctatcaggg tggcggccat
     5881 tgccgtaccc ggcgtgattc tggccctggg cctcgatccc actgatttgg cggacggttt
     5941 ggttgagatt ctgcacttct caccgcgatt cgtatacggc gggttggctg gattgcgcat
     6001 gtttacgctg ctgcaggaag attggcgcgc acttggcttg tcccagcgtt cgcgcggcct
     6061 tggtgacggt aacgccatcg cccgtgccct ctcccaagcg ttcggactgc tggtgctctc
     6121 catccggcgt gccaccaaac ttgccaccgc catggaagcg cgcggcttcg gctccggcgc
     6181 cccacgttcc caagcccgca tctcgcgtct gaagcctatc gactggatct tctacgtcat
     6241 ctgtctggcc gtcccgctgg cctccatagg cgtagccgtc ggcaccggga attggcactt
     6301 cgcctttcag ggtgtctgaa aggcggcaga ctggcacagt gcctttcagg gtgtctgaaa
     6361 ggcgacagat tagcacagtg cctttcaagg tgtctgaacg ccttatgatg cccccgctgg
     6421 cgggggctgt cggcgtagcc gactggggtg gttccagcct cgaccaccct cagtctcgcg
     6481 actgacgtaa tgacccggca gtagggtagt ggctatgccc atcaacgccc agattcttga
     6541 taatccgaaa tccgaccgcg tgcgccgcat cgccgatctg acccgcgcca aaggccgcga
     6601 acgttcgggc cgttttctta tcgaaggccc gcaatccgtg cgcgaggccg tcacttggcg
     6661 gccggacgtg gtgcaggacc tgtatgtaga ggtttcgcag gcgctggaac atccgcgtat
     6721 catctcctcc acgttggaga agatcgtcga aacctcgcag gatgccacga tttatgtgca
     6781 ctactgcacc ggcgacgtga tgcgccgcat cagtccggac gcccaaggca tcgcggccgt
     6841 cggcaatgcc gagtccatgc acgccggagc cgatgacgtc gaacttggca agaacggtcg
     6901 ggccccgcag attgccgcct tctggcaggt gcgcgacccg ggcaacgccg gcaccgtgat
     6961 ccgtgcggcc gatgcggccg gctgcgacgc cgtaatcttc gtggacgact gcgtggacgt
     7021 gctgaacccc aaggtcatcc gctccaccgc aggctccctg ttccatctgc cggtactcgc
     7081 catgagcacc gacgaattct tcacctggac aggcgaccat aatatggacg tctgggccgc
     7141 cgacgtatac ggcaccgaag aacgcaagcc ggaaagcctg cccgcagtac tggcggacaa
     7201 ggcggcggtc gaggctccga aagccgtgct gttcggcaat gaggcgcgtg gcttggaacc
     7261 ggacatcctc acgcgctgcg gccgcatcgt ctccatcccg ctttatggca aggccgaatc
     7321 gctgaacctc ggcacctcgg ccgcggtgat gctgatgtcg ctgtctatgt cgggtcatgt
     7381 tgaaagaatg tgaaaccggt aaaaacgctg tgaaacgctg agaaaacatc agacgaaagg
     7441 gttctcgtgg cagaacaaat ggtgttcgac gccgaccagg tgaccgctga ggttgccgag
     7501 ggcatcgaga aaatccagaa cgcctccaac ctggaggagc tcaaggccat caagacaacg
     7561 tacgcaggtg ccgattcggc gatgaccaag gccagcaagg ccattggctc cctgcccgcc
     7621 gaccagaaga aggaagccgg caagctcatg ggcaagctgc gcgccgactt cggccgcgcc
     7681 tatggtccca aggaggtcga gctcaaggaa gccgccgaga aggccgcgct tgccgccgaa
     7741 accgtggata tgacgctgcc ggtgagccgt aagcctctgg gcgcccgtca cccgctgcct
     7801 aagctgatgg aggacgtcga ggacttcttc atctccatgg gctggcagat ctcctccggc
     7861 ccggaaatcg aagccgaatg gtacaacttc gactccctga atttcggccc ggaccatccg
     7921 gcccgccaga tgcaggacac cttctacgtc aagggcaatc aagccaagga cgccgccggt
     7981 ttcgtcggct ccaacatggt ggtgcgtacg cagacctcct ccgatcaggt ccgtgccctc
     8041 ctgacccgtg gagtgccgct gtacatcgcg tccccgggcc gcgtgttccg caccgatgag
     8101 ctggacgcca cccatactcc ggtgttccac cagtgcgaag ccctcgccgt cgacaagcac
     8161 ctgaccatgg ccgacctgaa gggcgtgctg gacaagctcg ccgtcgccat gttcggccct
     8221 gaagccaaga cnnnnnnnnn ncgcctgcgc ccgagctact tcccgttcac cgagccgagc
     8281 gccgaactcg acctgtggtt ccccgacaag aagggcggcc ccggctggct cgagtggggc
     8341 ggctgcggca tggtgaaccc gaacgtgctg aagtccgccg gcatcgaccc ggacgtctac
     8401 accggtttcg ccttcggcgt cggcatggag cgcacgctcc tgctgcgcag cgacatcaac
     8461 gacatgcacg acctggtcga aggtgacgtg cgtttcgccg aacagtttgt gatgggggag
     8521 tgattcagcc atgcctatga tcgatatcga ttggctcaag gaccatgtcg aggttcccga
     8581 aggcctgacc tacgaacagc tcgccaagga ccttgtcaag gtgggtcttg aggaagagga
     8641 gattcactcc tctcaggtga ccggcccgat tgtggtcggc tacgtggtcg acgccacccc
     8701 cgaaccgcag aagaacggca agaccatcaa ctggtgccac gtggactgcg gcgacgaatg
     8761 gaacgagacc gacgaagacg gcaacaaggt gccgcgcggc atcatctgcg gcgcgcccaa
     8821 catgaaggcc ggcgaaaagg tcgtcgtcac cctgcccggt gccgtgctgc ccggcgactt
     8881 caagatcgaa ccgcgcaaga cctacggcca catctccaac ggcatgtgtg cctccgaacg
     8941 tgagctgggt ctgggcgaca accacaatgg catcatcctg ctgcgtcagt atggcttctc
     9001 cgaggctgaa tacgaggctc tgaagcctgg tcaggacgcc atgcacctgc tgcacctcga
     9061 ccagcctctg cttgagatca acatcacccc ggaccgtggc tacacgcttt cctaccgtgg
     9121 cgtggcccgc gaataccacc actccaccgg tgccgcctac accgatccgg ccgtggcact
     9181 caacgagaag gccccggaac ctgctgacta ccagcccggc accccggtgg acatcgatgt
     9241 tgaaatcgac gataacaatc cgattcacgg cgtgcccggt tgcgaccgct actacgcgcg
     9301 tatcgtcaag gacttcaatc ccaacgccca cacgcccaac tggatgcgcc gccgcctgat
     9361 ccgcgccggc atgcgctcca tctccttggc cgtggacgtg accaactacg tgatgcttga
     9421 cctcggccag ccgatgcacg cctacgacct cgacaagctc gaaggcccga ttgtcgtgcg
     9481 ccgcgccaac gagggtgaga aactcaccac cctggacggc aaggaacatg acctgagcgt
     9541 cgaagatctg cttatcaccg attcgccgaa cggcgaacgc ggctcgcgca tcctcggcct
     9601 tgccggtgtg atgggcggcc tgtatggcga agtgaccgcc gacaccaaga acattcttct
     9661 ggaggccgcg cacttcgatc aggtgaccat cgccnnnnnn nnnntcctga ccgacattgg
     9721 ctgcacggtg gccggtggcg gcaacggtga gttcgccgta accgcgccga gctggcgtcc
     9781 ggacttgaac gagccgtgcg acctggtcga ggagatcgcc cgactggtcg gctacgacca
     9841 gattccgatc accgtgccgc cggcaccggt cgagggtctg gtgggcctga ccccggacca
     9901 gcagcgtcgc cgtcgcgtgg cggacgagct cgccgagttc ggcatggtcg aatcgctgag
     9961 ctacccgttc gtgggtgacg acgattacaa ggccttcggc ttcgacccgg aagccaccaa
    10021 gaaggtcagc gtcgagatcg ccaacccgct ctacggcgac cgtccgtacc tgcgtcgcga
    10081 gattctgccg accctggcca ccaccgtgca gcgcaacatc cgccgcggca tcgaaaacgt
    10141 gtccctgtac gagctcggcc acgtctacct gtgggatccg aacgcgcccg ccatcccggc
    10201 actgcccggc ggcgtgcgcc ccaccgacga acagctcgcc gcgcttgacg ccggtctgcc
    10261 cgaacagccc gaccatgtgg ccggcatcct gaccggtctg gccgaggacg actggatggg
    10321 cggcaagcgc ccggtcgact ggtccgacgc cgtcgaggcc gtgcgccgca tcgccggccg
    10381 tatcggtgcc gcaatcgaac tcgaccagcc cgccgccgac gatgtgccag tccaatggca
    10441 ccccggccgc gccgcccgcg tcatggtggg cgacgtcttc accggctggg ttggtgaact
    10501 gcacccgcgc gtcaacgagg cgctcggctt cccggcccat tccgcggcct tcgagctgaa
    10561 cctcaccgcg ctgttcgcca cgctgaccgg caagcccgtc caggccaagc cgatctccac
    10621 gttcccgccg gtcaagcagg atttggcctt caccgtggac gagaccgtga ccgcaggcca
    10681 gcttgagaac gtgattcgca aggccgccgg cgcgaacctc gaatccatcg aactgttcga
    10741 cgtgttcacc ggcgaacagg tgggcgaggg caagaagtcc ctggcctatg cggtggtgtt
    10801 ccgttcaccg agcaagacgc tgtccgccga agacagcgac gccatccgca aggccatcgt
    10861 ggctgaggcc gccgaaatcg gcgcccagct gcgcgcctga cccaaccaat catccgaatc
    10921 gaggagcgcc atgagccaag cggagcagga caagccgaaa tacgggcagt accagcagcc
    10981 tgaatatggt gctatgtctg gccaatacgg gccgaattac aacccgtata tttatggcgc
    11041 tcccgagccg gatactaaga aggattccgc cgcagccgct ggcgataacg gtggcgcggc
    11101 acaatctcag caggctggcc agccgtatgg tcagcagccg gcgtggcccg gcaatcagca
    11161 ggcgggcggc tggccgtacg ctcaaggcgg ccaacagtcc ggtccgggcg catatccggg
    11221 cgggtacccg ggcatgcagg gccagccgta tggtcaacag ccgaacggtc agcctcacca
    11281 gccgcattat taccacggca tcgacctgaa cgatccaaac cagaacccac tctacgggca
    11341 ctgggattcg tatgcgatca tctcctttat cctcgccctg ttcttcccgg tgccagtgct
    11401 ctcggcactg atgggcgcgg tggcaatgtg gcgcacgcgc actttccata tgaagggctt
    11461 cggtttggcc gtggccgccg tggtaatcaa cgtgctgtac accatcgccg tcatctggat
    11521 ggccttcaat ggctatgacg ccatgagcct ttaccaggaa gccctccagc aactgaccgg
    11581 cggcaccggc tcctcctccg actccatctc cgcataagta gcgttcacat accaacacac
    11641 ccactgcata aatatgcaat agtctgcata atcgcgtata ctcatccgaa gacacatcag
    11701 tgaaaagaga tgagtatggc gaaatataca gtggccgtgg ccggtgccac gggctatgcc
    11761 ggcggtgaag cgctgcgcat tctggccgcg caccccgact tcgacatcac ctgcgtggca
    11821 ggccattcct cagtggggga gtcgatggcc aagcacatgc cgcatattcc ccaactggcc
    11881 aatctggtcg ttgaagatac cacgcccgaa gtgctgaacg ggcatgacgt tatcatcctc
    11941 gcactgccgc acggtgcttc gggcaagctc gcctcgcagc tcgatccgaa tgccgtggtt
    12001 gtggatttgg gcgccgacca tcgcctcgaa gaacaggccg cttgggatga gttctacggc
    12061 gccgatttct atgagcattg gacctacggc atgcccgagc tcatcaccgg caaagctgcg
    12121 gacggctcct acacccgcca gcgtgcggcg cttcctggca ccaagcgcat cgcaggtcct
    12181 ggctgcaacg tcaccgccac cacgctggcc ctccagcccg gcatcgccga aggactggtt
    12241 gaatcgcagg atatcgtggc cgatctggtc gtcggatact cgggtgccgg caagaatctc
    12301 aagcgcacca acctgcttgc cgccgaggcc ctgcaatccg ccctgccgta ttcggtgggc
    12361 ggcaagcacc gccacattcc cgaaatcctg cagaacttcg cccacgcggc cggcaagagc
    12421 gcggccgaag ccagcgaatt cacgctcggc ttcacgccga ttctcgcccc catgtcccga
    12481 ggtatcctcg ccaccgtgtc cgcccgcatg acggacaagg ccaagacact gagcgatgag
    12541 gagattcgcg ccgtatggtc caaggcctat gaaggtcagg acttcatggt gctgctgccc
    12601 gagggcacgc tgcccgccac cggcaacatc atcggatcca acgcagccca cctgcaggtc
    12661 gtcaccgacc gcaaggccgg ccgcatctac gcttttgccg ccatcgacaa cctcaaccgt
    12721 ggtaccgccg gacaagcggt ccaatcactg aacatcgcac taggcctgcc ggaagacgcg
    12781 ggcctgacca agatcggagt agcaccgtga gcgttacatt tgcacaaggt ttctccgccg
    12841 ccggcgtggc ggctggtatt tcatccgtcg aaggcaagaa ggacttggcc ctcgtggtca
    12901 acaacggccc gctcgacgcg gctgcaggcg tgttcacctc gaaccgtttc tgtgccgccc
    12961 cggtgcagtg gagccgtaag gtcgtcgccg acggtcacgt caaggccgtg attctgaact
    13021 ccggcggtgc caacgcctgc accggcgagg ccggttacgc ccagtccgtc g
//
LOCUS       DAWBMW010000027        12523 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_1283_length_12523_cov_2.524302, whole genome shotgun sequence.
ACCESSION   DAWBMW010000027 DAWBMW010000000
VERSION     DAWBMW010000027.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 12523)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12523)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12523
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1283_length_12523_cov_2.524302"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4863..4872
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7963..7989
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2502 a         3626 c         3824 g         2534 t
ORIGIN      
        1 gcccgaagct caactgcttc accgagcctg actatggcgt gcccggcaac gccgacacca
       61 tcggcatcat caccaatgac aagaccatca agaacaatcc cgacctggtc aagaagttca
      121 ctcaggccac tcaaaagggt tacgagtacg cgtacgccaa cccggatgac gccgcgcaga
      181 ttctcgtgga cgaagcccct gacgccaacc tcaagcccga attcgtcaag aagagcatgc
      241 aggttatcgt cgacggccag tactggggcg atccggccaa gatcaaggac ggctctttcg
      301 tgctcggcac caacgacttc aagggcgctc aggaatactt cgacttcctg gctgaagaag
      361 acgcatacac cgacagtcat gacaagataa ttcatgaggc cccgcaggcc aaggaccttg
      421 ccaccgacga attcatcggc aagtaatttc cgatattccg agtttgaatc tacgcaaaaa
      481 tgcgtccttc ccatgaggaa ggaacgcatt tttgcgtatc ggccgataag gttattcata
      541 gcggatcacg ctaacaagga gacgattcta tggtccaatg tcctgaccgt attattgcca
      601 gcatggatac gggtgttgat gatgcgttgg cgttggcgta tttgttgggg tcagcggatg
      661 agtgcgagtt gggcggcgtg attgccggtt atggcaatgt ggatgcgaac acggcttatg
      721 ccaatacttg cgcggtgctt gatttgtttg gtcgggcgga tattccagtg tttttagggt
      781 ctgagcatcc ttcttgggct gatgcgttta ttcctgatgc agggtgtgcc cagttccacg
      841 gggatgatgg actggggaat actcggctgg cagggtccgg ggctctggct gatggagctg
      901 gtgtgtctgg ggatgatatg tctggtgatg atatgtctgg tgatgatatg tctggggatg
      961 acatgtctgt ggatgtgccc gatgaggttg tttctgttgg cgggtatttg gcgggggatg
     1021 ttcatgctca tccggcgttg ggtgcttgtt ctcgtgtgaa ccgctctcgg tcggtgggcc
     1081 acccccagtc ggctatgccg acagctctcg ccagcggggg caaaaggagg tgctccgttg
     1141 atgctgcgga tgggattgag tatctgattg agcaggttcg cgagtttggc cgagacgtta
     1201 ccgtgctggc taccgggccg ttgacggatg tggatgcggc tattactcga gcaccggata
     1261 ttgcatcgaa actcaggctg gtgatgatgg gcggtacgct tacccaaccc ggtaattgtt
     1321 gggatgcggt agctgagacg aatatcatcc aagaccctga ggccgcgaat cgtgtatttc
     1381 attccggcgc agacatcaca atggtgggac ttgatgtgac tcaccagtgt ttgctaccgc
     1441 aatcggcggc cgaccggtgg cgtgcgacgg ggaccaagcg cgggcggttc ttggctgatc
     1501 tcgcggactt ttccatcaag gcaaatttgg aagccgaccc cgcactgttc tctggcggca
     1561 tgccgttgca tgatccgttg gcggcggcgg ttgcacttga ttcttcactg gtcgattgtt
     1621 ttgatcttgc gttgcgcgcg gagaccaata ccggtgattt caatggagta cgaggccgga
     1681 ccaccggaga cccggttggg ctggtgaatc attccatgcc gcatgtgcat gtggcacttg
     1741 gtgtggattc gggacgtttt ttggatgagt ttgtagagag gatggctgag gtgtgccggt
     1801 aactcccctc agtccgctac acgggcagct cgaccgcaat tacggacaag ctgccgctcg
     1861 cattcttctg gcttgactct cgtctcgcct tcgcctacaa acagtccacc gggcgtttgc
     1921 tttacggctc agctcgtcag agggggccta acaaggacta tttcacaccc ccagcaatca
     1981 aggaccggct tcgccggact catcattacc tggtcatgtc ctatcggcct cggccaggcg
     2041 gcgtgaacga acagtccact agactgttcg ttatttcacg ccgccaaatc ggcgatcgcg
     2101 gtggatgtaa tggtcgatgg agcgccatag cacgtcacgg ccgcagtccg ggaagagttc
     2161 gggcacgaag tcgagctcgg cgtaggcggc ctcccacggc aggaagttgg aggtgcgctg
     2221 ttcgccggaa gtacggatca ccaagtcgca atcgggaatg tccggattgt acagatggtc
     2281 ggcaatcatc ttctcagtca cacggtcgcc ggaaatcttg ccatcgcgca cttcgcgagc
     2341 gatggcggcg caggcgtcgg cgatttcagc acggccacca tagttgatgc agaacaccac
     2401 atcgatgacc ttgttgttct tggtgcgctc ctcggcggct tcaagctcgt cgatcaccga
     2461 cttccacagc ttggggcgac ggccggacca gcgcacgcgc acaccccact cgtccatctg
     2521 ttccacacga cggtggatga tttcacgcga gaaacccatg aggaagcgca cttcctgtgg
     2581 cgagcgcttc cagttttcag tggagaacgt gtacaggctc aaataccgta caccggcttc
     2641 gatggcaccg gcgatagtgt cgaacaccac cggttcggcg gcctgatggc cattggtgcg
     2701 gaccatgccg cgctgcttgg cccaacgacc attaccgtcc ataatcacac cgatatggcg
     2761 gggtaccttg ttcttgggga agtcgggaat acgagcgggg tcgctgaacg gagctgcggg
     2821 aatgtccaac gacgtgtaat caacgttctc aaaagccata ctcatgaatc tatcaagcgc
     2881 agcgaacgaa tcgggcgttc gaggtaatac tccccccaat cttcaaccaa ctcctgcaca
     2941 acccgctcca caggcgcggc atcaagcacc gaccaatcgc cacgaatcag tgcatcgaac
     3001 tggtttaaca cgaacggtgc gatacggcgc gcatcggtag tgtggtcggc ttcgcacatc
     3061 acgccgccgg acgcgatgga caggtacgcc ggctccgcct tgccgcatac cacgcaagtg
     3121 cccaatcgtg gcgtccaacc ggccaaggcc aaggcgcgca tcacgtagga gtcgcctatg
     3181 gcttgcgggg cgtgggattg tttggcgagc gcgttcaacg cgccgatgag cagccgatat
     3241 tggttcggca gctgctcatg ttcggtgctg gcgattttat cgatagtctc aacgatgacg
     3301 ttggcggcct catacgccgc ataatccgcc gcgatcggag caccataagc ggcgaccgct
     3361 tcagcttggg agaccacgtc aagcgagcgg ccttcagcga taagcacatc gactcgcatg
     3421 aacggctcga gcctggcacc aaagcgtgat ttggtgcgac gtacgccttt ggcgaccgcc
     3481 cgaatcttgc catgcccacg cgtcaggatg gtgatgatac gatcagcctc gcccagtttg
     3541 gaggtgcgca acaccacacc ttcgtctcgg tacagcgaca tctcatctcc tttccccgcg
     3601 acgcccggca gactccactc aatgaagtag tcagtaaata aacagacccc agaaagcgaa
     3661 caccaacagt atgtacagca tggtgaaggc caccagccag aacaggattc ggcccagacg
     3721 ggtggaggcg agtaccgggt cggcaccggt gacgatgtct cgaacggatt cgcgacgagg
     3781 gggtatctga atcaggccgc ggacggaagc ggcttctatc aggtgcataa agacgcggga
     3841 ccaagcccat agcaccagca cggacaccac ttggatgacc ggccagctcc aatacatgac
     3901 acccggcgtc tcggtaggtg cgccacccca tgcaagtttg accacgccca tgatcacctg
     3961 cccgagtccg gcgaagaaga tgagcaatgt ggcaagagtg gtgagggtca aagtcagcag
     4021 cgcattgccg aaaccatgtg cgaaaccgag taccacaggc cgatccggta tcagcatgcc
     4081 cgcacgtttg gcctcgcgct tctctcgccg ccatcgggca tccgccgcga ttttgcgacc
     4141 cagcgccacc agtgccagca cgatggatac gagcagcagc agtaccaccg tgacgtgcag
     4201 aatcacacca tatatggtgc cgacgcgtct tgccttcaaa gcgcccggca gaccgattga
     4261 ttggtacaga ttggtgccgg ccaccttttc gctggtctgc gtgagtccgg cggaagtgcc
     4321 gacggcccag tcgatgagat cgtccacata ggcgtccgca aacggggtgc ccgcttcgga
     4381 ctcatctccc aaacgcagca catggttggc gaccgggtag ctgcgcacgg tcacattcca
     4441 gttatcggct ttgtgggcgt tgtagagaat cgcacgcacg ccgtcgacct gcgccgtcat
     4501 cacgtctttc gaaccgtaag ccacataggt gggtacggcg taggcggcgg gtttgagtgt
     4561 ggcgaggtcg aaattgttca gtccgaacag gccggtgtcc gcgctgaaca cacgttggac
     4621 gatggattga tagccttcgt tcgcgccaac aagggtgaag tcctgggtga cgaaaaatcc
     4681 cagtgattgg cgtgggctga acaccatcgg gctcagaaga atctggaagg cgacgtccgg
     4741 atcgtcctcg agcagatacg aggagatcca tgtcgattcg gaggtggcat agatgccgac
     4801 cttggcctcg tctacatcgc tttggtctcg caggtattcg atgacctgat cataagcctt
     4861 ggnnnnnnnn nngccgaagc cataccggag gccacgtcgc caaaggagtt gtcacaggtg
     4921 ccgtatccgg caccgtgcat gaacaccacg ccggggcgct tgcctgatgc gttctgaggt
     4981 tcccgaatca ataccttgat agcttggacg ccgtccgcat tggccttgcc ccgagcaccg
     5041 gactccttgg tcagattgcc gtcgctggtc atgttcagcg tgatgtatcg ctcggtgacc
     5101 tcatacgtgc cttttgccgg cagcgtttct ccggacgggt tatcgaaggt gacttcggta
     5161 tcgtcggtga gcgtggccat cgactggccg gtgggttcga tgttccaagg caccgtggtc
     5221 agattggaaa tggagaccag cacgccaatc aggataatga agatgggcag gctgaccatc
     5281 agccggtggg tgctggtcca cggcttggat cgaacagtca ggcgatgctc ttcttcttgc
     5341 ggcacgtgcg ctattgtaat ctgaccgcga tatttatgcc gagggcctat tcggccgaat
     5401 cagtgaccgg ggtgctggat tgggaccacg accgggccgg tggaattgac ggggacttga
     5461 ccgtcggcct ccatttgggc ggcgatatcg ttcgctatgg tgagcagcgt gtcgacgatc
     5521 tggtcttcgg gcacggtctt gatgaccttg cccttgatga agatctggcc cttgccattg
     5581 ccggaggcca cgccgaggtc ggcttcgcgt gcctcgcccg gaccgttgac gatgcaaccc
     5641 atgacggcca ctcggatcgg cgcggtcacg tccttcaggc cttcggtgac ggcggaggcg
     5701 agctggatca catccacttg ggcgcggccg cagctcgggc aggagatgat gtcgaacttg
     5761 cgggggcgca atcccatgta ttccaagagt ttgcaaccca ccttgacctc ttcggccggc
     5821 ggcgcggaga gagacacgcg aatcgtgtcg ccaatgccct cggccaagag cgcaccgaac
     5881 gccaggcagg atttgatggt gccttgccaa gcagggccgg cctcagtgac gccgaggtgc
     5941 agcggccaat cgcccttgga agcgagcagt cggtacgttt cgaccatggt gatgacgtca
     6001 tggtgtttga cggagatctt gaaatcgtgg aagccgacgt cctcgaacat gtgcgcttcc
     6061 ttcaaggcag aggccaccaa tgcttccgga gtcgggccac cgtatttggc gtagagttcc
     6121 ttgtccagcg agccggcgtt cacaccgata cgcagcgaga tgcccgcgtc ggtggcggcc
     6181 ttgcagatgt ccgggccgac ttcgtcgaac ttacggatgt tgccggggtt cacgcgcacg
     6241 gccgcgcagc cggcgtcgat ggcctggaac acgtatttgg actggaagtg gatatcggcg
     6301 atgaccggaa tcggcgattt gcggcagatt tccggcagtg cgtcggcatc gtcctgactc
     6361 ggcacggcca cgcgcacgat gtcgcagccg gcggcggtca gttcggcgat ctgctgcaac
     6421 gtggcaggta cgttggccgt gagcgtgttg gtcatcgact gcacggagat gggcgcgccc
     6481 ccgcccaccg gcaccgggcc caccatgatg cggcgggact tgcggcgcgg gtgcaagggc
     6541 gattcgctgg tcaggtcgac cggatcaccg gttttgcgga acggcttttc gatgggattt
     6601 tccgtattca aggtattcat cattgcttgt ttatcaggtc gtcggcgcgt cggcgggctt
     6661 caagctcaag ctggttcatt tcctccacgt ccgtgaacag cgggttacca cgaagctcgg
     6721 cgtccatctg gtccagcacg gctttgaccg tgtccacgat gccgaggtat ggcagacgat
     6781 gctcgaggaa ggcatgcacg gcctgttcat tggctgcatt gagcaccgcc gtatgcttct
     6841 cggaggcggc caggcagtgg cgggccagtt gcacggcagg gaacgcctca tcgtccaacg
     6901 gttcaaaggt ccaagttgcg gcttgcgtcc agtcgcacgc ggcggccaca ttggtcatgc
     6961 ggtccggcgc ggacaggccc agcgcgatgg gcagacgcat gtctgggggc gaagcttggc
     7021 aaatcgtggc gccatccaca aattcgacca tggaatggac gatggactgc gggtgcacgg
     7081 tcacgtcgat gcgttcagac ggcacgttga acaagcggga cgcctcgatg acttcgaggc
     7141 ccttgttcat cagtgtggag gagttgatgg tgacaaccgg ccccatgttc cacgtcggat
     7201 gatgcagcgc ctgctcggga gtgatgttct ccatgtcggc acgtttccag ccgcggaacg
     7261 gtccgccgga ggcggtgacc acaagcttgg cgacttcggc gtgggttccg gagcgcaggg
     7321 attgccagat ggcggaatgc tcggaatcga ccgggttgat ttggttctcg cgcacttgcg
     7381 cgctgaacag cagatgcccg cccgcgacta cggactcctt gttggccaat gccagctggg
     7441 aaccggcctt gagcgccgcg atcgacggtt cgagtccgat ggagccggtg atgccgttca
     7501 atacgacatt cgcaccggag ccggccatgg cgatgaccga atccggaccg ccggtcacgc
     7561 ggacaccttg tgcgccggcc tgtgcgagag ctgcttgcag cgctggaacc ttggtctcat
     7621 caaacaccgc gacttcggac acatggaact gtgcggcctg ttgcgccagc agctcgatat
     7681 gtgctccgcc cgccgccaga ccggtgacgg taaatcgttc aggatgacgc gagatgacgt
     7741 caagtccttg ggtgccgatg gatccggtgg atccgagaat gatgacagtg gagttggagg
     7801 ctatgctcac tgttccttct tcgtatcgtc attgttcagt gtggggaacg acaggtcggg
     7861 gatatccgaa tcgaagttac cgccaagcgg gaacagcagc gggaacgaat cgctggaatc
     7921 gtgcttatcc tgtgacggct gagctgcttc cgtgtcatgg gannnnnnnn nnnnnnnnnn
     7981 nnnnnnnnnc agccggacgg gagatcgtgc tgtctgcagg ctgactatcg gatacgcgaa
     8041 ccggaatgga gttcgtgtta cgatccggct tgctggtggg agcaaaggag accggcatag
     8101 cagcggacgc agcctcgtca gcagtgctat tcacaggctg tactgccggg gcggagggtg
     8161 ccggggatga aaccgaaccc ggtacctcat tcaatttcag cgcattcggt gcatcaaggc
     8221 tgggcatctt aggagcgaag gacggtgcat ccactgccgg aagttcctga gaaaccgcag
     8281 ccatgtgggc gagagcggcc aaagaggaat cgttctgcac catatccacg ggctgttccg
     8341 cgaacaccga cggggtcgat gacggagctg cgggttcgtg agctgcctgt gccggagcac
     8401 tgacgggagg ctcagccgca gcaactgcgg caggagccac ggaaggcgtc tcggagacgg
     8461 gcttcgccgc agactcgttg gacgcatgca ccgggtatgc tgcgggagca aaggaaaccg
     8521 gctgttccgc attggtgcta ttggtgcttg tggaagcatc gaacgatgca ggagccgacg
     8581 cataggcgat cggtgccgcc gcggccggcg ctgcgggagc aaccggagcc ggagcagtga
     8641 acaagctctg ctcggcctga tgcagggcat cgaatgattc gtcatgagca acggcagccg
     8701 gagcaaaacg ttcgtcagtt gccgggcgct gcgttccgga agcgaacaac ggtgagacgc
     8761 cgttcgcttg aacggtcacg ttcgtggcgg tctgggcagg agccgcaggc tcgccggaca
     8821 cgaaatcggc cacacgggca taggattcca agcgactcag caactgtacg caggcgttca
     8881 ggaagtaatc gacctgtcgc tcgtcatagc ctttcttgcc cttgcgctgg gtgaagatca
     8941 cattgttgac cgtattcgca ttgagatcgg ccaaagaccg tacatcgtct tcggtaacgc
     9001 cgtcaacgcc caatgcggcc gccgccttgt ccacaatctg gtcgaccaga cgatccacct
     9061 gcttcttatc gtacgaaggc tgcttgggcg ctccggactt gaagcgttcg cgctcgttac
     9121 gctccgcatg gttctggact tcgtgataca gcttctcggt cttcgccttc cacgtcacac
     9181 gcccgtgctg tgcgatctcc catgtggtct gcttatcgac cacggcacgt tccagacgtc
     9241 caagcgcggc gtcaacctgt gcgatcacat agccgttctt gcgcaaatca aaggacacgt
     9301 tctgaatgtc atgctgggtg agattcatgc cctcgctgtc atacaaagca tgggcgcgct
     9361 ccaggaaagc gtccacctga gcggggtcat acccccactt gcgcttgccc gcacgtgcga
     9421 tgcccgactt gccgtcccct tcacgaagtt cctgcgccat cggctattcg acctcctgct
     9481 ggatataaac actctgccac aactttacgt gactattgag acgcatctga ccaagaagac
     9541 cgaaattcac gattttgtag cagtactgcc ggtttccttc tgcacgcatc tcattgagat
     9601 ttcagaaccc ttgccgaaga ctgtcaggga atatcgtcac agcctatatc cgtggaatga
     9661 ggggattcca tgaagcgatg aaaaatgaaa gtgggcaatg agggactcga acccccgaca
     9721 tccaccgtgt aaaggtggcg ctctaaccaa ctgagctaat tgcccgcaaa tgacaacaat
     9781 agcgtatggc cagcactgcg cgacacgtgg tgtcgcatac gcgtgccgta gcgctcaatc
     9841 gcaatgacat tcagtcacgc ccagcagcac tacgcagcac acgacgccgg tcaacacgct
     9901 taaagtcaag cgtattgatt gaggcttatc agcggccctt gccgaatgcg cgcaggcgga
     9961 tgccgttcca gtccgagctg acggtcagcg gggtggcggc gttcataccg gcggtctgtg
    10021 cagcggactg cacggtggac gagctggcgg cacccgggcg gccgggcttg ggagtcagta
    10081 tccacagcgg gccgtcattg cccagtaccg cgtaggcgtc aacgatcgta tcggacaatt
    10141 cgtcctcatc atcgccgtcg cgccaccata cgatgacgcc gtccacagcg gagtcgtagt
    10201 cctcgtccac gagatcctcg cccgtgagct cttcaatctg ggcacgaatg gaatcgtcca
    10261 cgtcgtcgtc ccagagccac tcctggacga tatcgccggg ctcgaaacca aattcttcag
    10321 cgttctgtga tgccgtttga ttcacagggg acacattagc aagtcttcgc gaacaaatgc
    10381 gttaacgcgg ccgaaaatcg ccgcattgtg ggcagaccca tgcgagtcgt ctcaccagaa
    10441 acaacgactg gccgcgcgtg tcatgcctcg caatgaggca aatcgtcggc ctgccccttg
    10501 ccgatggcca ccaacgcctg atacgcctcg ttcagcgtgg cgaccttgac gtcgcgcaga
    10561 ccatccggca cgcggcctgc cacatccaaa caattcgacg cgggggccag gaaccatgtg
    10621 gcgccatcgc gcttggcgcc aagcatcttc agctctatgc cgccgattct gccgactttg
    10681 ccgtccttgt ctatggtgcc ggtgccggcg atggtcttgc cgccgctctc gttggcgggg
    10741 gtgagtttgt cgatgagacc gagcgtgtac atcatgcctg cggacggccc gccgatgctg
    10801 tccacgtgca tcgtcacctt ggcgtgttgc aatgcactgg cgtcaatgtc cagttcgtcc
    10861 gcatgggctt tggcatacgc cagaccgacg gcactggccg aatcctggga accggccatg
    10921 tcttgttcga ctttcttctg atactggtcc gcactctggc ccaccggtac cgtggcctca
    10981 cgcggcatga cctccacctg cggattgccc caaccccaga cggcctgcgc gttgatgatc
    11041 gggtacccgg gcacgccgga cgcgttcacc gtggtcagca gcagcttgcc ggaatcctta
    11101 tacgtcgtca cgccggtacc agtgatgtcg ataaccgcac catcctcaac tttgcccagc
    11161 acatcctggg tgggtccggg catctcgatg acgtacgggc tgggcagcag cagaatcacc
    11221 acaccaagca acacggcgaa tgcaccgacg aaatggcgtt tcgaacgagc ggccgcatag
    11281 gcccgagcac ggctgattcc acgagatagg cgcgaatcat gctccacttc gaacgatggc
    11341 atgtgctcat ctcccgcttc cgaatccgca gtcgggctct cctgctcctg gacgaggtct
    11401 ggttcctgat tcctgtggcg tgcaaacata ccctacagtg taagcggagg gccgaaatcg
    11461 gccgaaaacg aacataaacc gaaaataatg ctcggccaca agcgaacagg cccgggcaga
    11521 ccctacactt ggggtaacac accgcggcaa agaaggatac gcatggacga caacgcgatt
    11581 cacgagtggc tgatcaaatg cttcggcccc attcagggtg agatggcctg gcagcagatc
    11641 agccagttgc ctgaggagat ccgtgaacag ctgatgagtc aggaccccag ccgactgccc
    11701 aatccatctg aagtccagca gatgatggcc gcgttcagcg ccggcggact caacacgatg
    11761 ggtgatatgc agcgcactgt cgaggaaggg ccgatcaacg tcaagctcgc caagtcgatt
    11821 gccttgcagc aggccaacgc ctccggttct gcatccagcg tgtccgccgt ggatggcgag
    11881 gcggctcgcc gcgcgatgag tgaggcgaac ctgtggctgg ataccgcctg cgagttcaat
    11941 cccgcacccg gcgatccgga tgtgctgacc cgcgccggct gggtggaggg caccatcgac
    12001 cagtgggcga agttcgcagc tcctgtggcc gagtcgatga acgacgcttt ggcttcggtg
    12061 atttcggagc gtcttggcgg cgcgttcggc gatggcgaga ttgccggcat gttcgccgga
    12121 ccggtgccga ttccgattcc cgacggtatg aaagacccag cccaactgat gaagctgctg
    12181 ggcaacacgt cctttgccat gcagttgggg catgcggccg gcaatctgag ccatgaagtg
    12241 catggcagct tcgatcaggg catcgcgttg ctgaagaacc cggctggtgg actgattgcc
    12301 cagaacgcca ccgaatacgc gaagatgctg aacggtgagg cttctgcggc cgaggccgac
    12361 acggccggca cttcaaccga ctcggtcaat ccgttctcca catccaccga cgcttctgcc
    12421 aatcccattg gcttcacttc ggccggcaac agcgatactc cggcaactga cggtaccgcc
    12481 caagtctccg gctctgatgc ttccgccgat accgccgacg ccg
//
LOCUS       DAWBMW010000028        11895 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_1357_length_11895_cov_2.997804, whole genome shotgun sequence.
ACCESSION   DAWBMW010000028 DAWBMW010000000
VERSION     DAWBMW010000028.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 11895)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11895)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11895
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1357_length_11895_cov_2.997804"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    544..553
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1909..1918
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5264..5273
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2033 a         3753 c         3766 g         2313 t
ORIGIN      
        1 cgctcaggct cgctttccag catggagagc gtggagcgct cggccagcgt ggtggcttcg
       61 accacgccga tgccggtgcc ctgcaccacg ttgccgatca gcttgccgag cgtgttgccg
      121 gggccgaggt ccacgatcca cagcttggac gggtcggcgt cgtcgaacag cgccttgacg
      181 cgggcgttcc agtccacgtg gttcaggagg cccaggccac ggtctgttcg acggcgtcgg
      241 ccatgagcgg cgagtggaag ggcagggtga cctcaaggta ttcgagggtc gggttgaaca
      301 cggtgccgcc gtggagcttc tgctcgcgca gcttgacctg atgctggtgt tcgcgctcgg
      361 cttcgagggc gaaagcggcc aggtcttccg ggtagccgga cagcacgtgg tggttgtcgg
      421 agttggtgac ggcgatggag atcgggccgc gagcgttgtt gacgcgggcc accagcgcct
      481 cgacctgggc gcgggtggcg cccttgacgg agagcatcgg gctggcttcg ccatacttgg
      541 ggtnnnnnnn nnngatcagg gcggccacgg ccagaatctc gtcaagcgtg gtgccggcgg
      601 cctcgatgga gccggcggcc tcgatggcgc gggtcatgtg cacggcgagc acgccctggg
      661 agtggccgag cacggcgagc ggcttggcct gggccacgct gtagccgagg tgttcgaggt
      721 cgagcagggc gccgagctgg gtcagcgcca cgccttccac ggacgcggtg gcgtcggctg
      781 cggcgccgag gcgtgccggg ttcggggcga agccgaacag gtcgaccgga cggccggtgg
      841 tggccagcag gtcggcggcg accggggtga gcagtcggcc ggctgcggcg acgtggccgc
      901 gcagggcctc gtcaaggctg ggatcggtgg tctggtctgc cagggctacc ggccacgggg
      961 tggactggcc ggcgaaggcg agcgcatggg gctcggcggc taggcgattg aggaagaagg
     1021 tggtcattgc tttccttact tgtgtttggt tgacgtcttc aaaaatcaga ggggctggtt
     1081 gccgtggtgt ttggtggtgc gcttgacgcg ccgcttggtg gcgagcgtgg ccagcgattc
     1141 gacaatcacc tcacgggtct gttcggggtc gatcatgccg tcgatctgcc cgatttccag
     1201 tgacaggttc gcgttgacgg tgctggtctc gtactccttg atgtacttgg cccgcagcgc
     1261 atcgacgtcc tgtccggagg ccttggcctt ggccaggtcg tggcggtgga tgatgttcac
     1321 cgcgccggcc gcgccgagca ccgcgatctg ggaggagggc cacgcgaagt tcaggtccgc
     1381 gccgatggcc ttggatccca tcacgatgta cgcgccgccg aacgccttgc gcaacaccac
     1441 ggtcaccatc ggtacctgtg cgttggcgta ggcgtagatc accttggcgc cgcggcggat
     1501 gatgccggcg tgttcctggt cggagccggg cttgtagccg ggcacatcca cgagggtgac
     1561 cacgggcagg ttgaacgcgt cacacagacg tacgaatcgg gcgactttct cggacgagtc
     1621 gacgtccagg atgccggcga gcacgttcgg ctggttcgcc acgatgccaa ccggcttgcc
     1681 gtcgatgcag gcgaagccga cgagcgcgga ggcgccgaac agttcctgca cctgcacgaa
     1741 ttcgccgtaa tcgacgatgc aacgaatcac ttcgagcatg tcgtaaggct gacgttcgtt
     1801 ggtgggcacg atggtggcaa gtcgcttggc ggtctcgcgt tcggcgcggg tgacggcgta
     1861 ggcgtagacc ggcggcttgc tttcgctgtt ggacggcagg taggcgagnn nnnnnnnngg
     1921 cgagcacggg gcgcgcatag tcgatggcgt cggattcgtc ctcgccgagg tagtgggcca
     1981 cgccggacac ccggttgtgc acttcgccgc cgccgaggtc ggccatggag atggtctcgc
     2041 cggtcgaggc cttgaccacg tccggtccgg tgacgaacat gttcgagttc tcacgggtca
     2101 tgatgatgag gtccgtcagg gccgggcagt agacggcacc gccggcgcag gggccgagaa
     2161 tcaggctcag ttggggcacg aagccgctgg cctcgcaagt cttgcggaag atgcgaccat
     2221 actgggtcag ggcggccacg ccctcctgga tgcgggcgcc gccggagtcc acgatggcca
     2281 cgatcggcac tttgaggtcg atggccatgt ccatcagtcg gcagatcttc tcgccttcgg
     2341 cggtgccgag ggtgccgccc ttgacggaga agtcctgggc gtagacggcc actttgcggc
     2401 cgtagacctg gccgaagccg gtgatgacgg ccgcaccggc gttgccgccg gcgatattgc
     2461 cgccctggaa gcggccgatc tcctcgaacg tgccggtgtc gaagagcagg tcgaggcgtt
     2521 cgcgcgcggt tttcttgcct ttggcgtgct gcttgtcgcg ggcgcgctct tcggcggcgc
     2581 gggccagttc ggcggccttg accacagcgg tgcgcagcgg ctggtgggcc gaaggctgtg
     2641 cggcgttggc ggccgaggcc gcagccgcgg ccttcacggc ctgcgaatcc atgatgtcag
     2701 tcattgccac cctccttctt ggcgtcggtg gccttatcgg ctccttctga cgagggagct
     2761 gtcgacgcag ccgcctgagg gagagatccc ggcgcagccg cagcagcctt ggccttgccg
     2821 gcgttcacgt ccagcgtgac cagcgtgtcg ccggcctcca cgccatcggc cgggccaacg
     2881 aaaatcttct tcacttcgcc ggcggccggc gcgtacacgt agttctccat cttcatggat
     2941 tcgagcacca ccagaaggtc gcccttggcc acttgctggc cttcggacac gttgatgcgc
     3001 gtgaccacgg cctgcatcgg ggaggcgatg acgccggatt tggcgccgtc gttggccgca
     3061 gcggcactgg aggccacgtt gtgcagtccc gcaccgcgca acggctgggt gggtcgcttg
     3121 gcaccgcggg cgcgggccga gccggtgatg ttctccacga tgtcgagtgg cacggtgagc
     3181 ttgacgcggc ggttgttcat ctcgatgacg aacgtctccg acttggactt gtcgggcgcg
     3241 gccggggcag cggcacccgg ggcggcggcc aaggaggccg gctggccgga ggcggcggaa
     3301 gccggtgtgc ggttcaggta cgtgcgctcc agccacttgg tggacacagc gaacgggtgg
     3361 ccatgctcgg cggtgaagtc gtcgttgcgg aagatttcct tgaacagcgg aatcggcgtg
     3421 ggcacgcctt cgatcacaag ctcatcgagc acgcggcgca cacgggccac ggcgtcgaga
     3481 cggttctggg cggtgacgat gaccttgccc atcatcgaat cgaacttcgg ggagatggtg
     3541 tcgccctgtt ccacaccggt atcgatgcgc acgccggggc cggccggcca ctggatcttc
     3601 tccaaggtgc cggagccggg ggtcaggttg gtggccgggt cttcgctggt gatgcgcagt
     3661 tcgaagctgt ggccgcgcag ttcgggggcg cgggtgagct cgccaccggc ggcgatattg
     3721 agctgttcgc gcaccaggtc gaggccgctg acctcttcgg agacggtgtg ctccacctgc
     3781 aagcgcgggt tgacttccag gaagtacacc ttgccgttcg gggtcaccat gaattcgcag
     3841 gtgcccaggc ccacgtagcc gaccgtggtg aacaggcggc gggagtattc ctcgagcgtg
     3901 ctgatgattt cctcggacag gaagggcgcg ggtgcctctt ccacgagttt ctggttgcgg
     3961 cgctgcagcg agcagtcacg ggtggagtac acggtgaagt tgccgtgcga gtcgcgcccg
     4021 gactgggtct cgacgtggcg tgccttgtcc acgaacttct cgatgaagta ttccttcaga
     4081 tcgccgcctt gcagcgcgtc gtggttcatg tagaagcgac gcagctcctc gtcgtcatgc
     4141 accacggtga tgccgtggcc gccaccgcca tcagtgcgct tcatcatgat cgggtagccg
     4201 tgggtgtgtg cgaagtcgag cagcgtgcgc acgtcggtca ccggttcgga caggccgggc
     4261 actggcggca ctttggcgcg tagggccacg cggcgggcgg taatcttgtc gcccaaatcc
     4321 accagcgcgg tgtggtgcgg gcccacccag atggcaccgg catcctcgac cttctgcgcg
     4381 aagctcggca cttcggaaag gaatccgtag ccggggtgca cggcattcgc accggtcttg
     4441 tgcaggatgt caatcagcag atcctcgttg aggtacgtgt ccttgtaggt gtcgccggag
     4501 agcaggtatg cctcgtcggc catgtccaca tagcggctat tgcggtcctg ttcggagtag
     4561 acggccacgg tggagatacc catctccttg gccgtgcgca ccacgcgaag cgcaatctcg
     4621 cctcggttcg caatcagcag ttttttgacg atagaagcca tagtgtcaaa ttgtgctctc
     4681 tatatagagg agagggcaag ccgcgttcac acaaaagctt tcacgccctt ttgtaggcta
     4741 cgaacaaacg cgcgcgtagg ttccggcaac gtaaccgttg gcgcctgcta tcaaaaacgt
     4801 tgcaaatccg ccgattgcac atccttgcgc gccggctcaa gaagttgacg ttacggttgt
     4861 gttcgtcaac ttatgacaaa tcgaaatcga caattttgta aattggtacg aaaccgcacg
     4921 aaatcgaaca cctctacgat ggcaaccgta agttcggggg aaggctcatc tgcgggcgaa
     4981 cggaaggtga ccggaccggc atcggtccat cgccgcacga ttcgcccgca cagggtcgga
     5041 ttcatttggg accgtccacc atatggacgg ttcggactgt cggggatacc gcacgacccg
     5101 ccggaacctc ccctcatcca tcgcataccg cccgccgttc acggcgatat cacgcaacat
     5161 aggggtcatt gatatgcaga cttcacacac caccgcatcc gctgcggcca ccacatcgct
     5221 cgcccgtcgt atcgtcgccg catcctggaa gccggtgctg ttcnnnnnnn nnntgtccgc
     5281 ggccgccggc gagattccga ttccgggcac gccggtgccg atcacgctgc aaacgttcgt
     5341 ggtgatgctc gccggtctta tgctgccgtg gcgtcaggcc ggtgccgccg tcgccaccta
     5401 tctggcggcc ggtgcggtcg gcctgccggt gtttgccggc ggcacctcca cgctggccct
     5461 ggtcggcccg agcgccggct tcctcttcgg cttcctgccg ggtgtggtgg tcatcgccct
     5521 gctgcgcggc aagtccgaca cttcgtcctt cgcggccgcc gcgcgcaccg cgggccgcta
     5581 cctgctggcc gcgctggtcg gcggcgtcgt ggtggtgtac gccttcggct tcgtcatcca
     5641 gtccgcgctg accggtgcgc cgctcgccgc cgtggccctg gcctccatgg gcttcgtggt
     5701 tggcgacgcc atcaaggccg tggtggcctc tctggccgcc gccggcctgt ccaagctcgg
     5761 tcgctgacct gagctactag ctagctactg gccggctatt acagcactcc cacatcggcg
     5821 gtgcggaccg tatggtccgt gccgtcttgt gttctcagga tcagtgaggc gtcttcgttg
     5881 aggccgatgg cttcgccggt cagcgtggtg ccgtccgtga agtgcgcctc gacctgacgg
     5941 ccgcgcgccc agcacaccgc cttcacctcc tcgcgcaacg actcggcctg gcccttcggg
     6001 tcggcgatga acttcaccaa ccgctcgcgg aagccctcga cctgacgttc ggccaccgca
     6061 tcgcgcatct cgccgaacga cggcagcccg gagacatgca gctgcagcga ggtcgattgc
     6121 ggcgtgggca gccgacaggc gcccaaggcc aggttcaggc cgacgccgaa cacgatgacg
     6181 acgtcgtcat cggcatcagc atcagtaccg gtaccggacg acggcggaat caccagttcc
     6241 gagagcagcc cgcccagctt gagcccgtgg cagaacacgt cgttcggcca tttgagttcc
     6301 agcgagcagt ccggctggtt cggcgcggca ccgtattcct cgatcatgcc gttcagggcg
     6361 tccagcgtga ccagtccggc gatcatctgc agccagccat tgacggattc gtcggtggcg
     6421 atcgcgcgcg gaatgcgcac ggcataggac atcgtcgagc aacggccggg ctgactgacc
     6481 cacttgtgac cattgcgccc acggccggcg gtctgccggt cggcggccac cacggccacc
     6541 gccagttcgc catcctggtg gtcgggcaac gtcaacgacc cgtcgccaat catctgtacg
     6601 gccagcgcgt tggtcgaatc ggtttcgtcc acggcgacca catgatctgc gacggctgta
     6661 gttctgggta gctgcggcgt tgcagctttc aacgcaatca tcatgggcac tacgttacct
     6721 cgattttggc gcgatatgcc cgcgttattc catggaaaag cgcgcaaaat caactatttt
     6781 cacccacata gcccatgtta acgttcacaa cttcggaaat tccgcattcg ccgaccccac
     6841 gcatgcaccc ggccgtcata ttggcttgcg aacaatggcg atgcccggca gacatcgaat
     6901 atcaaccgtt gacctgtgcg gctaccatat attgtcatga cgtcaccagc caccgcgaag
     6961 cttgccgccg tatccagccg ccagtcccac ccgcatcttg cgcgcgcggg cgctgccgcg
     7021 cgctggacac tggccgtgct ggccggcctg tggcttgcac tgtgcacggc ggtcggcccg
     7081 ctgtactggg acgaggagaa cggcaccctc gcccattgga actgggccaa taccgcaata
     7141 ttcgtcgtgt cattcctgat atatctcgga atcatcacgc tgatggtgcg attcgcagca
     7201 ggccagcgca tactgccgag ggctatcggc gagcgactac gtcaacatca gcggattagc
     7261 gaaacatcac ggcccgccgc cgatcagcag gccagccaac cagccaagac ccgtcgtacc
     7321 gttcgcgcta tcgtcacgtc caccgtcact gttctggacc gctggatcac acgcggcacg
     7381 aaccggttct ggaagcttat gctggtgttt tttgtcggct ggctctgggt gccgaccact
     7441 ctgctggccg cattcggtgc ggacctccgc tcacagatcc gtgaattcag ctgggcctgg
     7501 aaccagtgga ccggcctgaa acagccctat atcggcttct tctccttcgt gccgatggac
     7561 atctacccca ctgcgcacta catgtggccg tccgatccca cgtatctgac cgaccagcac
     7621 aatgtggtac tcactgtgtt ctatggcgcg atggtcacgt ttgcgaggca tctgaccggc
     7681 tcaaacgatg ccggtatcgt gacgttggct gccctgcaga cgctgttcgc cgtgttctgc
     7741 tgcgctgccg ccgccaatcg attcctgaac cggccttgga taggaaaaac ggcaacggat
     7801 tccgccgctc ccccacaggc cggaggattg gctcgattcc tgattctgct gttctttatg
     7861 gtctgcccgc tggccgtgtt ttccactatc tccatcacca aatcgccgct gttcgcgttc
     7921 tcgttcgtct ggtggttcag cgtctggtat gaactcgtcc agacctggca ccccgccggc
     7981 acccgcaaac atcctcagac ccctgccatc gccacgcccg tccacttgcc gcgtcacagt
     8041 ttcatcgcct tcatcctggc cacgtcggta atgctgattt ccgcgaaata tgcgtggtac
     8101 atcatcgccc tacaaatcgt gctggctctg atcgccgacc gcaagcgctg ggcgacttac
     8161 gtcgtggcgc tgctcatccc caccgtactg attcacggcg gcatctcctt cgccatctcc
     8221 tctggcgcga tcatcggcgg cgacccaatc gaaagccgcg gcgtacagct gcaaatgatc
     8281 gcccgcgtgg cccagcgcaa tccggacggc atttcagacg aagccaaaaa gaacctcgcg
     8341 cccgtcttca atctcgatca gatggccgac gcttactccc agcaggacgc cgacccggtg
     8401 aaatcctccg gcattcaggc caagaaggtc agctacaagt ggcgcacggt gacgcccgag
     8461 gatatgacca atttcaacaa ggcatggttc gaaatcgtca aggacaatcc aatcatcgcg
     8521 ctggatgcgt tgctggccaa atgcttcgga tacttcaatg tgaacgatca gccgtacgta
     8581 tcgatggatt attacgtgac cagcgattac gtgcagaaaa actccacatg gatcaaggat
     8641 tacaaccatg actggcgtga gcacatcgcc ggcttcactc gcgtatgggg cggtattccc
     8701 gtactcggct ggcctacgca cggcaatttc tacgtggtga tgacattgct gatcggcgcg
     8761 gccgaggtga ttcgccgacg ttggctcacc ctcatgaccc atattccgct gctactgctg
     8821 atgggcgtga tgatcaccgc gccggccaat aatttcgagc ggcacatgtt gccggttgcg
     8881 tttgtattcg gattcgtggt gctgacgtat tggcgagagt ctttggctga aaggcaacgc
     8941 caatcagcga cgctacacta gtgactatgt cagattttct cgaacttctt gctgccgacg
     9001 ttgccgattc gtccgcaccc ggcatcgccg cgaccaaccg cgtacgcacc gtgctgttcg
     9061 agtgcctgtt caatcatctg gccgacgaac gagtggagtc cctgttcacc atcctcggct
     9121 tcgagcatga cttcgattgt tacgccatcg ccggataccc tgcccggtcc gccgcacgta
     9181 cggccaagga aatcgagaaa atcgtagccg atttcggcgg tgtgtgtctg accgccaagc
     9241 gcccgatcgg caacagcacc gccgtagtgg cgttgattcg tccggtcggt gcggccacgc
     9301 cggaaatcat ctgcaatacc atcgacccgt cgttcgccgc cgatcggcca atcgccttgg
     9361 gtccgcagcg taccggtgcc gatggcgcga tgcgttcgat tcaggccacg ctttactgtc
     9421 ttcaggccgc gccggctctg gccgccaccg tgcagccatc gccgcgcccg ttacgcaccg
     9481 acgacgccct gcccgaacgt gcgttgatcg gcgacgcgga cgcccgtgac gaactggtcc
     9541 gcgtggtcta cggaagcctg acagcagcag gcccggatga tccgacgctg ctcaccgtct
     9601 ccacattcct gaagttcggc ggttcgctcg agaccacggc caaggagctc aacgtacatc
     9661 ccaatacgat ccgctaccgt ttgaagcgcg ccgccgaatc caccggctgg gatgccaccg
     9721 acccgcgcga ggcgtacgtg ctgatcaccg ccatcgcgct gggtcgtgtg gccgaagcgc
     9781 agcggaccgc agcgtagtcg ggcagaagca taaccagcag aaccgtagcc ggcaaaggcc
     9841 atagctgacc gggcataacc gtccagcccg acagcctagc gggccgaatc aggctgcctg
     9901 aatctcgtgt acgccatcgt gcagtaacgc ggccaaggat tcttgagtgg ctaacacttc
     9961 gtccacgaat ccaccggcgg acaggctggc gatcacgcga ccggactcgt tgtccacaat
    10021 cggtacacca acggcaccga cacccaacgt cacatcctca tccgacagtg catatccacg
    10081 ttcgcgagtc aagcgaatat cctcgcgcaa ctgttccaca ctgaccagcg tgttctcgtt
    10141 atatgccgga aacggcgctc ctgcaagata gcgttcggca tcggcatcct cgaaatacgc
    10201 caaggccacg cggccggccg cgccagcatg cagaggcagc acgcggcccg ggcgcaggat
    10261 cagcgaatcg atggcatgac cttgcgcgca ttccacgcat accaattcag tgccggagac
    10321 cacactgagg aatgaggtca tgccggtttg cgcggtgatg gcatgcatga ccgcaatcgc
    10381 aggctgccat tcggtacgag acgtgcgagc ggcatcagcc aatcgcagcc agtccagtga
    10441 taatgagtag ttgccatccg tatccgttac ggcccacccc acatcgacca gtccatccag
    10501 cacacggtat acggaggatc gcgggatatc acaggcgtcg gcgatatctg ccggagacag
    10561 gccattctcc gccgccaatg cgtccatcac acgactcgcc ttgtctaaag cgcgggtcgg
    10621 ttcacgtttg tcttccgcca tcgccgacgt tccttccccc tgacttatcc ctgactcatc
    10681 gtattcaact gctcaggcca agataatcac cgctgtatcc aaggcctcgc gacacctcgc
    10741 ggccggcagt ctgcaccaat tgcaccatgt cttgagccga tgccgttgcc ggtttcccat
    10801 ctaccagaca ggccagctca ggttccaagg agcgggaaag gccactcagg gcgattgccg
    10861 cctgaatctc tccggcgtga ttgaataccg gtacgctgat agtcacggag ccggccatgg
    10921 tctggccgac atcataggtg aagccgcatt cacgtatatg acgagcctgt cccattgctg
    10981 tggtacgcat cgtggagaat gtgccgccga gctcgttgtt ataggcatag tgttccaaca
    11041 gctgctcgta gcgagccggg ggaagccagg ccgtcagtac atgagaggga gcgctcatgc
    11101 tcagaggcaa gctattgccc accagctgtg catagctttg caatgcctca cgggtgcgtg
    11161 tctcgataca caccgtgcgc aatcgcctcc gaacgaacag accccagatg ccgaactggc
    11221 cacgatgggc gcggaaggta cggcgagcga tctgctgaat gtccagtctg ctttcgattt
    11281 ggccgccgat acgtacgcaa tcgatgccaa gccgatactc gccacgattg atgccgcgtt
    11341 ccacccaacc gactgcggcg agattatcca gcaaacgata cgttgagctt acgggttcac
    11401 cagtacgttc gcttatggtt tttgcagtac ttggtcccag ttccgccaat acatcaagta
    11461 ctgccttgga tttcaccaat gcatctacgc tacggtcatc acggcttggc gccaccatgc
    11521 gcgtcacctc ctggcctgta tgttgatacc tcagccatct taccgggtat tcagcgggtt
    11581 gccgcccaaa acgcactggg ccccggcagg agtatcccgt cggggcccaa gaggtttctg
    11641 gctcccctct ttgaggggga gccgtccgcg cggcggaccg gggggcccgg gcgtcgggct
    11701 cccacggtcg cggacgcgcc gcgtcgttcg gcctcgccgt cggggctggc cgggaatggt
    11761 cccgggcgtg ggtctcatgg gtatgcgggg aggtcccggt gcccgtgcgg gttcgggccg
    11821 gggccggttt cggatatagg gaaagcccct tggggcgtgt gctccgaggg gcttcctgta
    11881 cgtgtggtgc ggcgg
//
LOCUS       DAWBMW010000029        11781 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_1373_length_11781_cov_2.520723, whole genome shotgun sequence.
ACCESSION   DAWBMW010000029 DAWBMW010000000
VERSION     DAWBMW010000029.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 11781)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11781)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11781
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1373_length_11781_cov_2.520723"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    11488..11497
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2234 a         3531 c         3556 g         2450 t
ORIGIN      
        1 gagttcctga accgtctgga cgacatcatc gtgttcaagc agctcaccga gccgcaggtg
       61 cgccagatcg tggatctgga cgtcaagcag ctcaacgacc gcctgttcga ccgtcatatg
      121 tcgctcgaac tcaccgacgc cgccaaggac ctgcttgcgc agaagggctt cgacccgctg
      181 ctcggtgccc gtccgctgcg ccgcgtgatt cagcgcgaca tcgaggacgc catctccgag
      241 aagatcctga tgggcgatct cgaagacggt cagcgcgtga ttgtggatgc ggaaggcgag
      301 ggtatcctcg gcgaattcac cttcaagggt gagaagttcg aggagccggc cgccgccgat
      361 aagcccgccg aagacggtgc cgccactgat ggcgagactc cagccgacgc cactccggcc
      421 actgagccgg ccgaatccgc tcccgccgat tccggtgacg ctccgcagga gtagtccgtt
      481 tcgatagcgt agctagcgca taccagcaaa gctccccttg acaggggagc tgtcagcgaa
      541 gctgactgag gggtagacct gcgcacacat gatgtgcacg tttgtggtct acccctcagt
      601 tgcctctggc gacagctccc ctgtcaaggg gagctactgt ttgtgtcgga ttattacagc
      661 agggcgagtc cggtgtcgat ggtctcctcg cgggtaaaca gcgtgcggcc ggcgcgcacc
      721 gaacgcaata ccgagcagcg ctcatacacg gcgttgaaca cgctatcggc gtccagcaca
      781 atgaagtttg ctggcttgcc cgtttccaag ccgtacatat cgcgcaggcc caacgtagtg
      841 gcaccgttga cggtcaggaa cttcaatgca tcgtcgattt cttcaaagct catcatctgt
      901 gccaaatgca gcacataatc aagaatgagc atcatattgc cattgcccaa cgggtaccac
      961 gggtcctgca ttgaatcctg gcccaatgac acattgacac ccgcctcagt gagctccttg
     1021 actcgcgtga tgccacggcg cttggggaag gtatcctgac ggccctgcaa atacaggttc
     1081 tcggtggggg cgcaggcgaa gttgatatgc gccgccttaa gcagcttggt caggtggaag
     1141 aagtaggcgt tgtccgccga tcccagcgaa caggtgtggc tggcggtgac cttcgggcca
     1201 atgccagcct tatatgccag cgccgaaagc agctccacgt aacgggaatt cggatcatcc
     1261 gtctcatcgc aatgcacgtc gatgagcttg tcgtatttcg aagccaactc aactaccgtg
     1321 tgcatggaat gctcgccaaa ctcacggcac tgttcgaaat gggggatgcc gcccacgcag
     1381 tcggcaccca tcttgagtcc ttcctccacc agctcggcac ccgattcgcc atgcgggccc
     1441 tcgtaggagt acatgccctc ctgcgggaag ctgacgatct ggagagtgac cgtatccttc
     1501 aattcctcct taagctccag cagcgccttt aacgaggtca ggttcgggtc ggtcacatcg
     1561 gcatgggatc gaatgagctg cacgccatgc agcatttctt tcttaatgcc gattttcgcg
     1621 cgctgcttga tctcgtcgac cgtcaagtcg gacttggtct cactccatcg ctggatgccc
     1681 tcaaacaagg ttccgctttc attcaccgca ccgggcttgc gggccgtgaa tacgtaatca
     1741 aggtgcagat gcgtatcgca gaacggtggg cagacaagtt tgccgcccag atcgaccacc
     1801 tcaatggccg ccagatcacc accctgagcc tcatattgtt gcgcgagtcc ggggccgaat
     1861 gcggtgaaca caccatccgt gacgagaatg tcctgcaatg gcagggtgct tgcagccgtg
     1921 cttccgggtt ctccggtggc gatatgtgcg ttggtgaaca gtgtggtagt catcgtcgct
     1981 ccttcaactg gtgtgttgcc gaacggttac gtgaaccgac tgcccgcgta accaactgag
     2041 gttattgtca acagcacgct ttgcggtgag gttaaccgga acgaccggtg ttccgcgggg
     2101 gaatggacag attgtatgac tgtatcgatt cgcagtgtgc ttcataacac gcagttcaaa
     2161 cgagccgatc ctgtggtgga ggtcgaaggc aaagacctcg atacgccagt gcgatgggtg
     2221 ttcaccaacg agcgagagga tgtctccagc ttcctttcgg gcggtgagat gctgattatc
     2281 gagggcaacg ctctgctcgc cgccgattgg cacggcacgc taggacaata tgtagcaagc
     2341 ctggcacagg ctggtgtcgc cgctctggtg gtcgaactgg tggagggtgt ggtgcggatg
     2401 ccggacgaac tggtgtctgc ggccaggctg catgggttga cgttgattgg tttgaaatct
     2461 cgcgtacctt ttgtcgacat ctgtcagagc gtcaataccg ctatcgtgca cgaacagatg
     2521 cacttgcaat tggaggtgga cacaatgtcc acttcattac gtgagggatt gtcccgtacc
     2581 ggcaatatcg aggctgtggc cgaaaccatt gcgtcgctgt tcggcgaaag cgtcgccatt
     2641 ttcgatggtg acggtctgtt ggccgctcga gcgggtcgtg cgttcgatgc cggcaacgaa
     2701 tcgagcgccg tcatcgccct tgaaagtcgc tcccggccgg tgggagcatt ggagatcacg
     2761 caacgcacca tgacttccga cgccacgatg cgtcgggcca tcgccaccat cgtgtctccg
     2821 gtggccgccc tttatattga cggcggtgcg cgcatgggca tgatgcatca tctgagtatg
     2881 ggtccggccg acggcgtgca cgtcaataca tttgaggcgc aggaagcgca tgcgatgctc
     2941 gaagcgctgg gttttgccgg atcatgcatc tatatgccat tcgcttttcg gttcagatcg
     3001 gtggtggaag gcatcaacca tgtatcgacg atggtcgaac ggttcgaaga actgtcggga
     3061 tgcgccgtct cctgcatgct ggaaggtgac ttgatggtcg gatggtgctc caccaccgat
     3121 tcctcagacg gtgcggccgc attcggacgc cattgcatgg atgcgcttgc cgtactggaa
     3181 ggcgacggta tatacgtggt gcatggccga gtggcattgg acacggtgac gctggtcgac
     3241 gggttcgcgg tattgcgtga catcgccaat catgaaccgg catacgggcg agtcgtaagt
     3301 accatgtccg cactcgtcga ccgtttcgtc gcatcctctg atatggacaa ggcgatgcgc
     3361 atgctcgtgg tgcagaccat cggattcgag gtcgccgcct ccccgctgtt gctggacaca
     3421 ctgtgcgcct gcttcgacaa cctcgacagc aaaaccggcg agtgcgagca attgggtatc
     3481 agacggcaaa ccctgtacaa ccgactggac aaagtgacac aaatggtcgg cgtcgactat
     3541 aacgacaaac cgaactgggt gatgctgcta ttcgccgcca aactcacgaa atcatggcaa
     3601 gaacagcatg gtgaataata cggcagtgcc actgggcaac ctgtccactt ctacgccgaa
     3661 ctggttaatc gacgattcac gtttcagcaa atactcaagg gcatagtcga ggctatcgcg
     3721 gcgctggtcg acctgctgcg aaacggattc ccacttgaat ctcattcagt cacacgaaca
     3781 gcacatcctt gaaaggacgg tgacatcatg agttccgcaa cgattactac atctgcacga
     3841 ctgatgaatt ccaccccgca aactgaagaa caggccatgg tcagaaaagt cgcggtatct
     3901 tccttcctcg gcaacttcat cgagtggttc gactacgcct cctattcgta cttcgccacc
     3961 actatcgccc tggtgttctt cccaaccgac aatcacaccg tcgccatgct gcagaccttc
     4021 ggcgtgttcg cgctatcctt catcctgcgt ccaatcggcg cactgttctg gggtagctat
     4081 ggagataaga aaggccgtaa agccgcactg gcccactcga ttatgttcat gtccggtgcg
     4141 agcttcctga tcggctgcct gccgtcttac tcggtgattg gcgttggcgc gccgattctg
     4201 cttctactgc tgcgcatggt gcagggcttc tccgcctccg gtgaatatgc cggtgcggcc
     4261 acgttcctcg gcgaatatgc gcccacttca aagcgcggca tctactgctc gctgattccg
     4321 gcttccaccg ctatcggtct gctcgccggt tctactctgg ccacgctgat gaccgccaac
     4381 atgaactcta gcgcgatggt cggttgggga tggcgtgtgc cgttcctgct ggccggcccg
     4441 ctcggtctga ttgtgctgta catccgcgcc aagctcgctg attcccccgt ctaccagtcg
     4501 atgaacgatg cgcttgagtc caagggcgcc gcctccgccg acggtcaaat cgctgccaag
     4561 agcggcatgt tcgccccgct cgccgagttg ttccacaacc atctcaaggc tctgatcatc
     4621 agcttcggag cctgcgtgct gaacgcggtc ggcttctaca ccgtgctcac ctacctgccg
     4681 acctatctgt ctgacaccgt cggcatggac gccccccagt ccggcaccat caccaccatc
     4741 tgcctggtcg cctacgtgtt catggtgttc ggcatgggcc acatctccga caaattcggc
     4801 cgcaagaagg tgctcatcgg cgcttgcgtg gcgttcatcg tcctcaccgt tcctgccttc
     4861 ctcatcctca acacctccca gttctggccc gtgctgctgg ttgagctggc aatgtgcgcc
     4921 accctgaccg ccaacgacgg taccctgtcc agctacctga ccgagacgtt ccccacctcc
     4981 gtgcgcttca ccggattcgc gttcagcttc aatctggcca acgccatctt cggtggtacg
     5041 gcctcgttca tcgccactgt cctgattgcc ctgaccggtt cgtccatcgc cccggcctgg
     5101 tacatggtgg gcgttgcggc catcgccttg gtcgcgatga tcctctccca cgaaaacacc
     5161 gacaaggatc tgaatcacat ctgatctcct cagctccggc tccttccccg agggagccgg
     5221 gcgtattcat cgaccatcca atccaccacc aacctaaagg agtactgcaa tgggtagcaa
     5281 ccgccgtatg gaccacatga cctggccgga attcaaggag gcgaagactc gtcccgtcat
     5341 cgtgccgatc ggttcgaccg aacagcacgg ccagcatctg cccatcggca ccgacgcggt
     5401 actcgccacg cgggtcgccg aagatctggc ggaacgcatc gacggcaccg tgctgcccac
     5461 cctgagctac ggatacaagt ccaagccgct ctccggtggc gggccgctgt tcccgggcac
     5521 tatcgacatg aacggcgtga cagtgatcaa ccaaatgcac gacgtgctga gcgaactcat
     5581 cgccgatggc ttcaccaaga tcgtcgtcat gaacgcgcat tttgaaaacg aggcgttcat
     5641 cgtcgaggcc atcgacctgg tcacccgcga aaccggggga gtggccacca tcgtggaaac
     5701 caattggtgg gacccggttc cgcagtcggt tatcgacaag gtattcgatg gattggtgtt
     5761 ccccggctgg gcattggagc atgcggccgt caccgaaact tcgttgatgc tccactatgc
     5821 gccggaactg gtgcacatgg accgtatggt cgaggaagcc ggtgccacgg cgaaatccta
     5881 tgtccgctac cctgtgcgcc aaggtgacgt tcccgctcac ggcggactcg ccaatccggc
     5941 cggctcgagc gccgaacgcg gccgtctgat tgccgaggcg tgcgtggatg cggtagctga
     6001 gatctgcgcc gaggaatttg gcgagtaatc tccccttgct gacgcattcg tagtgactcg
     6061 ggttgcaaag gcctgaccct gatagtatgg ggtcaggcct ttcccatccc aagacgccgc
     6121 taagaattca cctgagagtc gccttcctag gcatggccat tcaatcgacg attctagcgt
     6181 gactggtgtg agcaggaata tgaagaaccg gcggtgggcc ggcaagcgat cggggatcgc
     6241 ttttggtgca gtagcgctta tccttacggc tctggtggtt ccttatgctt gtgcgggaac
     6301 cacgggagcc gttccttttt ccggcgataa tccatcttcc ggcacccgta cggtgaccat
     6361 cgccgacatc actgattttc acgggcatat cgaacgtggt gcagacaacg ccaccgcctt
     6421 caccgtggcc gacagccata atcccggcaa tatgattccg gtatcgaccg gcgatttggt
     6481 gggaggctcc ccgtatgagt cggcggtgga gaaggaccaa cccacgctgg atatggccaa
     6541 agcgtgggga ttgacgattt cggcgatagg caaccatgag ttcgaccgcg gtgtcgccga
     6601 tttcaacaat cgtatcgctg atcccagcaa cggcatcgac tggctgtgcg ccaacgcgtc
     6661 tgccgccaat aagtcgcctg acggactgct gagccacgta cgtgactcta cgattcgaac
     6721 ggtgaacggc aagcggatcg gtttcgtggg cgcccttact gatgcgctcg gctcggtggc
     6781 cactccgcag atcactcgtg atgccgacct tgacgaacgg gcggtggacg cgatcaatcg
     6841 tgtggcccgt gaactcaagc gctccggaaa ggtggatgcg gtggtggcct tgctgaatgc
     6901 cgatgcttca gccgccgccg atattgggcg agatgttgat gtggtataca ccggtcacag
     6961 tcacgcgatc aagcacggta caaccgcggg tggcgcgcct atttacgagg cgggcagctt
     7021 cgggcggaac atggccgtgc aggatttgat catcaccggt gccgggcgtc gcgccacggt
     7081 gcgagtggcc gacgttgacc tcggcaatgg caccagccat acggcggttg acggcgtgct
     7141 gggcgtggat gggcttaatg cgcatccggc gcaagccgct tggatgtctg ccggtgctga
     7201 ggagaacgat gaggtatcgc gcgcgcagca tatttacgag gcgtcggcac catatgcgaa
     7261 ccataccggc agcgcggtga ttggcacctt ggcctccggc gtgaatttcg acaaacaagg
     7321 ttcggacaag cacgggaata cggtcggtgt gctggtggcg gacgcgaacc gagagtcaat
     7381 catgaagcat gtgtatgccg ggaatcgctt gccggtaatc ggattttcga ataatggcag
     7441 tctgcggact ccacggcttg atatgaatgg cgatggcaaa gtcaccgtgc gcgaggtgga
     7501 tagtctcatg gccttgcagt tcaaggccgc gcatgagacg ctcaccgggc gcgatgtgaa
     7561 gagagtgctc gccgagcaat ttcgccgtga cgatgggcga ctggagcggc gctggctcgg
     7621 catctcctcg aacgtgactt atcggtatgc cgaattcggt acggctggtg agagcggcaa
     7681 tgccgatgcc ggcgttgata ctgattccgg cgctgatacc aacgctgacg ctgacgagtg
     7741 tgctgccgac gaacatgcgg cggttcgtat cgcgaacctc gccgttgacg gccgaccgat
     7801 tgcggacgat gacctggtga ttatcgcgtc caattcatat ctgcttcagg gcggcgacag
     7861 ttaccccgcc ttccgtgccg gaaccaatta cggcgagctg gatatgccat acagccagcc
     7921 gttgcatgaa tatctggccg cacatcaagg gctcacggca gtcgttgcag taccggtagg
     7981 gactcaggcg taagacacca attgctgggt gactgaggaa ctatggggtg aagttatgag
     8041 tatagggcag gagaaaacgg cataaatatg acgaagctcc cctcgctgag gggagctgtc
     8101 gccgtagacg actgagggga gcttgcggta taaccgtaaa ctcccctcag tcagccttgc
     8161 tgccagctcc cctctctgag gggcacctgg cgagaccctt acttgatggc gttcagccac
     8221 tgctccttag tggccttctc ggcttcgagc ttggccttct tcttcgggtc ggattcggcc
     8281 gcgatcttgg cgtcgagctc ggcgagctgg gcgttgagct gctcctcgaa gctggacttg
     8341 cgggcgtcgg cctccggatc ggtctgcttc caagcggcat cctcaatggc cttgatctgc
     8401 ttgtcaacgg cgtcgagacg gttctcgatg cggcgcatat cgtcacgcgg cacgtaaccg
     8461 atctgatccc actcttcctg aatcttggcc agctcctggc gggccttctt ggcctcggcg
     8521 tcggtcttca ccggcaccag agcctcggcc ttgaccagca gagcctcctt ggcagccaga
     8581 ttctccttct cggaggagtt gatctgatcg cgatcggcct gacgagcgtt gaagaaggtg
     8641 tcggcggctt cgcggaactt ggcccacagc tcgtcgtcct cgttacggcc agcgcggccg
     8701 gccttcttcc aacggtccat cagatcgttg aacttgcggg aagtctcacc ccaagcggtg
     8761 gaatccttga gctcattggc ctcggcgata atcgcttcct tggcttcctt ggcggctgta
     8821 cgctcgttgt cgcgagcctg agcccacttg cggcgagcct ggttgaaggt ggtacgggcg
     8881 acggagaagc gcttccacag cgcctcggcc tccggcttgt cgatgcgcac ggtggtgcgc
     8941 tggtggttct gccactcgtc gaacaggtta cggaacttgt cggcggtgga gcgccagttg
     9001 gtgttgtcgc ccagagaagc ggccagtgct tcagccttct cgacaatggc ggtgcgctcg
     9061 gcaacggcct tggcctgggc ggccttgcgt gcctcggcga tttcggtctt cttggcctcg
     9121 ccggcagcct tcagctcctc gtactgggtc ttgagtgcgg caagatcgcc cacgacggct
     9181 ggctcggagg tttcttcacc caagagcttc acggattcgt cgatttcacg ggccttgatg
     9241 ttcggggaag ccaggcgggt ggccagcaga tcgagcttgg ccttgagatc caggaaacga
     9301 cgggcgtaca gtgccaacgc ctcttccttg gagacatccg ggaactggcc tacctcgcgc
     9361 tcggcgtcgc catccttgac gaagacggtg ccgttgtcat ccacgcggcc gaaggcctcg
     9421 gcggccttga catcagcctc ggaataggcg gcggtggcgg cggccggggc ggcagcgcgc
     9481 ggcttggccg ccggagtctt cttggcgaag gcggcggggg agggcgcgtg cggtttcggc
     9541 atggaagcgg gggacggagt cggcgccggc gtggccgcgc tggcctcggg tgcgggtgtc
     9601 gttgcctgct cttcgggcgc agcggtgttt acgggttcgg tgacagtttc gtcggccatg
     9661 gccagctcct taaagcgtag aatgcataag cgggagcgtc tcccatcccg ggcgaaccag
     9721 gaagttttga cgcatccctc acctattata ttgatttcgt ggctaaaggt gcatcaatat
     9781 caggattccc ggagtggctc ccctccgaac gtgttgtgga gcagcgtgtt atcgatacgc
     9841 ttcgtaaggt ttttgagctc aacggcttca tcggcattga gacgcgagcg gtggaaaccg
     9901 gcgcttcttt gctgaaaaaa ggcgagacca gcaaggagat ttacctgctg agccgtttgc
     9961 aggaggtcgg tcatgaatcc gacactccga ttgaagagcg tttgggcctg catttcgatc
    10021 tgaccgtgcc gctgagccgg tatgtggtcg agcactcggg tgcgttggcg ttcccgttca
    10081 agcgctggca gattcagaag gtgtggcgtg gcgagcgtcc gcaggaaggc cgcttccgcg
    10141 agttcgtgca ggccgacatc gacgtgatcg gcgccggcga cctgcccgac cactatgaag
    10201 tggaactgcc gctggtcatg gtctccgcac tggaagagct gcgcgcgtat ggcctcccca
    10261 aggccaccgt gcacgccaat aatcgcaagt tgtctgaggg gttctaccgt ggccttggcc
    10321 tgacggacgt cgaaggcgtg ctgcgcgaga tcgacaagct cgacaagatc ggtgccgatg
    10381 aggtggcccg actgctcacc gagacctgcg gcgccaccga ggcacaggcg cgcgcctgcc
    10441 tcgaattggc cgagctcacc gcctccgacg gtgccgagct tgccgctaag ttcgatgcgc
    10501 tgtgcgaggc tcacggcatc gtcaaggatt ccgaggccta cacgctggct cgtcagggac
    10561 tcgataccct ggccatgatc gtcgacgaag ccgccgccat ccgccccggt tccgtcatcg
    10621 ccgacctgaa gatcgcccgc ggtctcgact actacaccgg ttccgtctac gagaccttcc
    10681 tcgacggtgc cgcatcactc ggctccatct gctccggtgg ccgttacgac aatctcgcct
    10741 cccagggcaa tcgcaagtat ccgggcgtgg gcctgtccat cggcctgagc cgactggtca
    10801 gctacatgct gcacaccgcc ggcgctcacg ccaaccgtgt cagcccggcc gccgtgctgg
    10861 tcgctgtgtg gaatgaagag gatcgcccgg ccgccaatcg tattgcgaac caactgcgtg
    10921 cgcgcggcat cgctactgac gtggcaccca ccgcggccaa gctcggcaag cagatcaagt
    10981 acgcggacaa actcggcatt ccgtacgtgt ggttcccggc caccgctgcc gaaggtgcgg
    11041 aaggcgcaga gcccgctggc gacgaggtca agaacatcgt cacgggcgag caggttgccg
    11101 ccgattgcac gtcgtgggag ccggatactg tggttgccca gcaaaccgtc gaaatctgac
    11161 gtattcgcgc gaaaaatcga ggagaaacaa gacaatgagc cagacggctt acagaacaca
    11221 tcatgccact gaagtgaccg aggccctggt cggccagaag gtcaccctcg ccggttgggt
    11281 tgaccgacgc cgtgatcacg gtggcgtggc tttcatcgac cttcgtgatt cgaccggcct
    11341 cgtgcaggtc gtcatttacg acgaggatat ggcccgcccg ctgcgcagcg aattcgtgat
    11401 tcagattacc ggcgaagtgc gcctgcgccc ggatggcaac gagaacaccc atctggcaac
    11461 cggcaagatc gaggttgtgg cggagacnnn nnnnnnngtt tccaccgctc ttgagaatga
    11521 gtccgagaac aagctgcctg gcgaggacgt gcgtctgaag taccgttacc ttgacctgcg
    11581 ccgcccgtcc atgcagcaca atctgaagct gcgttccgac atggctaagg ccgcccgcca
    11641 cgcgctggaa gacatggact tcaccgaggt cgagaccccg accttcatta agtccacccc
    11701 ggaaggcgcc cgcgacttcg tggtgccggc ccgtctggcg cccggttcct ggtacgccct
    11761 cccgcagtcc ccgcagctcc t
//
LOCUS       DAWBMW010000030        11677 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_1389_length_11677_cov_2.889864, whole genome shotgun sequence.
ACCESSION   DAWBMW010000030 DAWBMW010000000
VERSION     DAWBMW010000030.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 11677)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11677)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11677
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1389_length_11677_cov_2.889864"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3234..3243
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5420..5429
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6410..6419
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2306 a         3551 c         3408 g         2382 t
ORIGIN      
        1 acaactgtct tcctcgccgc aactacgcac gccaacagca ccaatgcgaa catcgatccg
       61 aacagcaacc cggtcttaag actgtcgcca ttggcagagg ccaccagtcc cactacggca
      121 gggccgccgg cgcatccgag gtcgccggcc acggctagta gcgcgaacat cagcgtaccg
      181 ccaccgggca tggcgtctgc agccatcgag aacgttccgg gccacataat acccactgaa
      241 aaaccggtga gcgcacaggc aagcagaccc agtaccggtg ccgtggttag ggctgcggtc
      301 agatacatgg ccacgcacag tacggaactg ctggcaatga atgccgtgag atccagtcga
      361 tgccccagca ctccgtaaat cgtgcgggac aggcccatca tcagggcgaa tgcggcaggt
      421 cccgccaagt cgccaatcac cttggtcacg cccagaccgg actcggcgaa cgcgctggcc
      481 cactggctca tgccctgttc ggccgcgccc gcgcacagca tcataaggaa catcaggtag
      541 aacaccggct tcttcgacaa gtcggcgaac cgcatggtcg ccgtgccttc cggcacaatg
      601 cgcggcatgg gagcgccagc gaacatcgcg atgccgatgg caggcacaat cgcccacaag
      661 caagcgagaa ccggccatga gccggtgccg aacgcaaaca gaaacagcgt ggagatggcc
      721 acggtgccga gctggcccca gcagtagaag gagtgcagca ggctcatcgc cttggccttg
      781 tgttcgctcg ggcaggcctc gacgatgggg gagaccatga cctcgatgag tccgccgccg
      841 agcgcgtaga ggaaaatcgc cgccagaatc gcgagatacg ggtccggcac gcgggtgggg
      901 agcaagccca acgccagcag tccggcaagg gcggccaggt gggcggcgat aatgcacggc
      961 ttgtatccga tgcggtccac gaacttgccg gccagcacgt ccacgaccag ttgggtgacg
     1021 aagttgacgg tgatgagggc ggaaagccgc gccatgtcga tgccgagtgt cgccgcgaac
     1081 gtgacgaaca gcaacggcat gaagttattg attaccgcct gagtgatgta tcccacgaag
     1141 caggcggtga cagtgtggtt gtagttcggg gctgtggcgc tcatagagtc agtattgcag
     1201 aaaaagcacc cgcgaaatgc gcgtgcgaag agaatcgtgt gggacggcag atgaacaatg
     1261 gtaaaaaccg cgtgccgtcc cacaaaaagg aatcaggcct gccaccattg gcgcaggggg
     1321 taccacttgc cgtccgggtg ggccacgcga tccggcttga cggccaagaa ctggtgaatc
     1381 tggatgccgt cgagctcgaa gttcagagcg caggcggcca tgtacatacc ccataccttg
     1441 gcgcgttcaa agccgacctg cttgaccgcg tcgtcccaat gctcggagag gttctggttc
     1501 cagtgatgca gggtgagcgc gtagtgctgg cgcaggttct cctgatgcac cacgttgaag
     1561 ccggcgtcat ggatgcacga ttcgatgaag ccgggcgcgc cgagatcacc gtccgggaag
     1621 atgtaacggt cgaggaattc gtcggtaccg ggcttgccgt gaggcttgtc atggctgatg
     1681 gtgatctggt ggttcagcag tcggcccatc ggcttcagaa ggcggaacat ctcttcgaag
     1741 tagctctggt agttcttgac gcctacgtgc tccatcatgc cgatcgagca gataccgtcg
     1801 aagtcgtgct caggcacctc gcggtagtcc tgcacacgca cttcggccag atcctgcagc
     1861 ccttcacggg cgatccattc gttggcgtac gcggcctgtt ccttggaaag ggtgacgccg
     1921 agcgccttga tgccgcgacg ggcggcggtg atgaccatcg aaccccagcc gcagccgatg
     1981 tccagtaagc gctggccggg ctgcaagtcc agtttgtcga gaatcaggcg cagtttgttg
     2041 gcctgtgcgt cttcaaggct catgtgttcg ttgtcgaata ccgcgcaagt gtaggtcatg
     2101 gaggatccaa ggaagtcggc gtagaactcg ttggacatgt cgtagtggaa gctgacggtt
     2161 tccgaatcgg ccttttcggt gtgcggcatc aggccacgct tgatacgggc gaacttgctc
     2221 gggccctcgg tggcgggcac cggcggcttc ttgaagccat ggctcaggat gccggcagat
     2281 acgcgggcaa ttgatgtggg agtgagtggg cgaacgtatt tggacagcga caccagcttg
     2341 cgcatcgccg gatacgggtc ggcgtagtca atgccgtcca cgtcgaaatc gcccagaatg
     2401 tatgagcgca cgatgccgat ttcattcggg tgcgcgagta gctggtacat gcagttcggc
     2461 gtggcgattt tgaccttcaa atccgcgtct gccgggccga aatgcgagcc gtcgaaagcg
     2521 tcgatacgaa cgggagtggc ggggtcgaga aacagggacg ccatctcgcc gaccgtcata
     2581 gccttgccat ttgccattat ctacctcatt ctcttatgat gatcacccca actgtcgagg
     2641 tatcttagtc ctcttcagga gactgaaacc gttgaataca ccatctggtg aaccagttcg
     2701 cgttcggctg atgaagcacg aatggaatta ggcatctcgc atattgattg cgctacgcgc
     2761 gaagtaagcg gatgagagac gagtttctcg gagagcgtca gatgactccg gaaaatggtc
     2821 ttatcgttga gatttcgggc gatggaactg gtttggtcaa tggacaataa ttcgacaagt
     2881 gcggaattcc gccatttctt gccattattt tcagtgttgt tctttcagcg tgttttcagg
     2941 tgaatgctca tgcgcggagt agactgggga accgttggac cactcaaaga cagagtggac
     3001 caccagttaa gacactagct caaaaggaga gcccctatgg tgtaccgcat gattttcaac
     3061 cagactgcat acttcggacg tggcgcgatc aaggagattc cggccgtcgc caagcagcac
     3121 ggcttcacca aggcatttat cgtcaccgac ccggtgctgc tggagaccgg caccgccgag
     3181 aaggtcacca aggttctgga tgaggccggc ctgccgtacg aggtgttctc caannnnnnn
     3241 nnntcgtcgc tttcgtggac ccggatctga ccgactcgat gccgcgcggc ctgaaggtcg
     3301 ccaccggact tgacgccctg acccacgcca tcgagggtta cattaccccg ggtgcctgga
     3361 gcctgtccga ctgcctgtcc atgcagacca tccgcatgat tgccaagaac ctggccaagt
     3421 ccgccgacgg cgacattccg gccggcgagc agatggccta cgcgtcctac atcaccggta
     3481 tggcctactc caacgtgggc ctcggtctgg tgcacggcat ggctcacccg ctgggcggcc
     3541 gtctgggcgt ggcccacggt gtggccaacg gtattctgct ggccccggtc atggaataca
     3601 acaaggactt caccggtgag aagtaccgcg acatcgccga tgcgttcggt gttgaggacg
     3661 cctacaccgg cgacctggag aaggtgcgcg aagaggccgt gcaggccgtc cacaagctga
     3721 ccgtggatct gaagaacccg accacgattt ccgaagtggg cgccaccgag gccgaccttg
     3781 agccgctggc tcacgatgcc ttcaacgacg tgtgcacccc cggcaacccg cgtcaggcaa
     3841 ccgaagagga catcctcgcg atttacaagt cgctgatgtg atcagtcaat gacagtaaac
     3901 attagctccc tctgatgagg gagctgtcag cgtagctgac tgagggagag aagttgcggc
     3961 gtagccgctg aataataggt atcggcctgc gtccgattca atgttttagc gactgcgtcg
     4021 cagtctcttc ctcagtctca cttcattcga cagctccctt atcagaggga gcttgcataa
     4081 gcagaggggg ctcgtatatc tagctacttt tcgtgctttt cgtcgtagac ttcgtcggtg
     4141 ggggtctcgt cgtacagctg cttgccttgc gcctccgcca gttccatctg ctcgttgatc
     4201 cacgcggctt cagccggatt gatatccgcg gaattcagca ctttctgcca caccgggtag
     4261 aacatgtgca tcgccggata catggcggtg aacaacacct ccggcttatg cgtgcgcgta
     4321 tgttgcggat gcgcatcaaa cgtattctta aaacgtcgca tgtagctcag gaacgaactc
     4381 aacgacgtat ccatccgtcg cgcactcatc gcggcgatca tgcgcggcga gtacacggtt
     4441 ttcagatcct tgcccatcag atgcagggaa atatcgatat cctcatgcat cacatcggcc
     4501 ttatcccggc agacctcatt cgcaatctga tgccaggccg aagcgcgcaa ggccatattc
     4561 gagccgaaca gcagcggctg gccgccatcg gcgcggtaga ttcgcttgcg caaggaattg
     4621 tcgcctttca acccgaaatg gcgtgagggc atgtcgtaat acatcaccgg accagtggca
     4681 cccatcgcgg ccgggtcttc ggtgaagatg ccggagacca cctccaccca gtcaggccgg
     4741 atcatacagt cggcgtcaaa acggcccagc acatcgccgg tcgcggcgtt caggccaaag
     4801 ttgcgggtgg gaatcagccc ctgttcatcg ttctggtgca acagtttgac cggggcctca
     4861 ggatgttcgg caatgaactg ttcgacaacc gcacaggtgt tgtccgtaga acgattgtcc
     4921 accaccagca cctcatgcgg catgatggtc tgacgggtgg cgttcagcag gcagtcgcgg
     4981 atgcgttcgg cttcgttcca cgctgggatg atgatcgaaa cgttgagcat aacatcaaca
     5041 atagaaaagc gggtgtacgc cgccggcgta tccgagcttc aaacccctcg ctacaatggg
     5101 gagccatgag cgaacgcgtg cgaaacgtaa acagcaccga cacgttgaga accggtgccg
     5161 cagaggccgg ggcagacccg gacgacgaga atcgtatcga ctttggtact ttgttccccg
     5221 ccaagggaga ttcccgtagg ccaccggaat ggttcggccg cgcgctgctg tatgtggccc
     5281 ttgccgtcat cgtattcatg ttctgctggc gctcgtgggg cgacatctcc tatctagtgc
     5341 tcgacatcat catctcgctg ttcctggcgc tggccgtcga accgttggtg gtggcgctgg
     5401 tggctcatgg ctggaagcgn nnnnnnnnnc gtggcgtggc ttccgccgtc agcttggtgg
     5461 gcgtggccgt ggtcgtgggt gtgctgctca cgctatttgg caacatgttc gtacagcaga
     5521 tgattgccat ggtccgcggt ttgccggcca tgtatgagca gattcgtgag ttcgtggacc
     5581 agtatgccac gttcaagatg ccggaaatca gcaatctcgg catggaaatc ttcaacaata
     5641 tccagacctc gtgggtcacc gattttgccg gtaccgccat gagtacggtg ggcggcctgt
     5701 tctccttcct gctgaacctc atgaccgtgg tcatgaccac gtattacatc tccgccgccg
     5761 gccccaaact gcgccgctcc ttctgccagt ggctggcccc caacacgcag cgccgattcc
     5821 tgctggtgtg gaccgtggcc caaggccaga tttcgtcctt cctgttctcc cgttcgattc
     5881 tggcgttgat caacgccacc tgcactgcca tcttcttaga gattctgcat gtgccctact
     5941 ggctgccgtt ggccctgttc tgcggcgtgg tgtcccagtt catccccacg gtcggcacct
     6001 atatcggcgg cgcgctgccg gtgctgttcg cttggggcaa ccgcggatgg acgtacgccg
     6061 tggcggtgct ggtgttcatc atcgtctacc agcagatcga gaacctgatt ctctcgccga
     6121 ggatttcgca gcgcacgatg gatatcaacg cggccgtcgc cttcctcgcg gtgctggcgt
     6181 tcggctcgct gttcggcgcg tttggcgcgt tcctggcgct gccggtcacc gcatcgctgc
     6241 aggccatctt ccgtgcgtac accaagcgct atgagctgat tgactcacca ctgatgaacg
     6301 atccgactcc ggagaagaag tccaagatcg tcgaggcgtc cgaagcgttc ggcgagcatg
     6361 tgctgcagcc gatcggcgag cacatgccgc gtgcggccaa ggggtccacn nnnnnnnnng
     6421 aacagatcta tgcgattccg ccacacgaga cgaaagaatc cgatttggac gattccgcca
     6481 ccgtggcgat tccgcagcgt ggtgccgcgg atacgcagca gtctgagcag cccgagcaac
     6541 gcaagcaaac caagcagtct gatcaatctg cctccagcaa tcctagggca gggtggcgct
     6601 gatgactctg ctttccgtac tcgatattct tctgatgatc gtcggcggtg ccggcatgat
     6661 atatcaggga ctgtgcatcg tgatgtccct gttcgccaag cccgtgcgct tccccgacgc
     6721 cccgatgacc aaccggtatg ccgtgctgat ctccgcgcgc aacgaagaaa aagtgatcgg
     6781 caaccttatt gggtcgattc gcgatcagac ctacccgtcc aacctcatcg acatctggct
     6841 ggtggccgac aactgcaccg acaacaccgc cgacgtggtg cggtctttgg gctgccatgt
     6901 ggtggaacgc cacaatcttg agctggttgg caagggatat gcgctgacgt atctgttggg
     6961 gcatatgctc gacatcgtgt tcgatgccga taacaagctg gacaagcact acttcgagga
     7021 aatgaacaag gcgttctgct ccggattcaa gattctgacc agctaccgta attcggtgaa
     7081 tctggcggat aactgggtgt cttctggttc tgcgctgtgg ttcatccgtg aatcgcgatt
     7141 cctgtctaac tcgcgtatga ttctcggttc ctcttgccat gtgggcggta cgggattcat
     7201 gttctcccgc gaggtgatga agcggaacaa gggatggaag ttccatctgc tcactgaaga
     7261 tcttgaattc actatggatt cgattctgca tggcgaccgc atcggctatt gcggtactgc
     7321 gatcctctac gacgagcagc cggttacctt ctcgcaaagt tggcgtcagc gtttgcgttg
     7381 gagcaaggga ttcctgcagg tattccgtta ttatggctcg gcattgctga aacgcgcggt
     7441 tcgcgaacgt gatttctcct gcatcgactt cacattgatg ctgtgtccgt ttaccgtgtt
     7501 gggcgcgatt cgcgcggtga tgggcttgct gttcgccgcg tgtggcttcg tgagttggcc
     7561 gagccagctg caatcgctgg ccgattggtc cagcggcatc atcatatcgg tgttgggcct
     7621 gatggcattg gctgcattga ccatcattgt cgagcgcgac cagatcggtg ccacgaacaa
     7681 ggagctgctc gcctatgtgc tcagcttccc gatttacata ctcagctacg tgccgatctc
     7741 cttccaggcc atcttcgcca aagcccagtg gaagcccatc gcgcataagg gttgaccgag
     7801 tccgctagtc cggagctgct cagcggggca atccaacagc aacccggggg tttgcttcgc
     7861 tgagggttga ctggtggggt agagtggatg tatgagctta gacaatactc aaaggaatgt
     7921 cggatttcag ccgccgatga tgccagccgc cgcccccgcg cctgcacagc cgtcgcaacc
     7981 gcagccacaa gcatggccgc cgaatccgaa tgcggcggtg tccattcgtg gcctgttcaa
     8041 gcacttcgac cggaagatcg ccgtcaacgg actcgtgctc gatattccgg tcggctcgtt
     8101 ctatggactt gtaggcccca acggcgccgg caaaaccacc acgctcaata tggtgaccgg
     8161 tctattggta ccggacgccg gcaccgccat gattttgggc catgacgtgt ggagcgacgt
     8221 gaacacggcc aagcgcatga tcggcgtcat gccccagccc gaccagatct tcgaccgact
     8281 gaccggtttg caattgctcg tctactccgg catgctgcgc ggcatgagcc gcgatgagac
     8341 cacgcgtcgc gcccaggacc tgctcaatgc tttcgacttg gccggggccg ccaacacgat
     8401 ggtcaccgat tattcggccg gcatgaccaa gaagatctgc ctggcctccg cgatgattca
     8461 tagcccgcgc attctggtcc tggacgagcc gtttgaatcc gtggacccgg tgtccagcgc
     8521 caacctcaaa gacattctca tcgaatacgc gaagactggc ggcacggtga tcatctcctc
     8581 acacgtgatg gcattggtcg aaaagatgtg tacgcatgtg gccgtaatca acgacggtct
     8641 ggtatgcgcg gccggcacgg tggacgaggt cgctgctggc gaagaactcg aagaccgctt
     8701 cctgcaacta gtcggtggcc gccacgaagc cgcccatctc gcgtggctcg atggcgggca
     8761 ggcccaagcc caagcaccaa cttcggccca gacgattcag ccgattctgc ctacgcccgc
     8821 cacacccacc gatgaggccc gccaatgatc aagaccgcca tcaccctggt ccgcctgcgc
     8881 tggacgctca cgctcgccac cctgcgaaaa tcggcgtggc agaccgtcgc ctatgtcttt
     8941 tccgctgtac tcgcggccgg caccatcatc ggcaccggcg tgctcgcatg gttcatcggc
     9001 atactgccgc ccttcaccgt gaacgcggat ggctttccgc tgaacatcct tggtcccgga
     9061 ggcatcgtca acatagtcgt cgtgctggtc ggcgcgacca tgaccatctt catcggcttc
     9121 atccagctca tgatgcttgg cgaaggctcc accatgaacc cacgcaaatt cgcgatctac
     9181 ggcatcgccg accgcaacct gcaattcggc ttgctgcttt ccggcctgtc cggcatcccc
     9241 tcccttactg gcgtggcctc actgatgctc tggacactgg cctatcggca tatgggacca
     9301 gccgtaatca tcagttccct cctcgccgca gtgctggccg tcatcaccat gatgagcatc
     9361 tccaaactgg tcatcgccct atccaccacg ctggtgacca gcaaacgcgg caaaggcgcg
     9421 ttctacatct tcatcgtgct gactttcgtc atcatctgcc agttgccgaa cattctggtc
     9481 aactccggca tagccttcag cctcaacacc gagacctggg gcgcggcgac cgccatcgcc
     9541 gcatggacgc cgttcggtgc ggccttccaa ctgccgttcg acgcgcttac cggagcttgg
     9601 gcggtgctga tcgcccgcat cgccatcctt gccgtcacat ggatcgtctg cttcgccgta
     9661 tgcacttggt gtctgaggca tgaacggctc accatcggcg caaacagtcc gtccgagacc
     9721 gccaaaggca tcggcgcatt cgcatggatg cccgattcgg catccggcgc ggtgtccgca
     9781 cgcctgatca cctatctcaa acgagacccc cgccaagcca tgaccttcgc catgccggtc
     9841 ctgttcctga tcatcttcgc cttccaggcg cacggcatca gcgcggtcgt ctggcagtcg
     9901 ctgatctggt gtggctggtt catgtcagtg gtggaaagca acgggctcgc atatgacggc
     9961 cgcggcttca ccatggaagt catcgccggc gtgcgtggcc ggaccgacag aatcggccgc
    10021 gtgcgcgtct acgtcggcat catggtcgtc tacatggtgc tgctctatct cgccggcatc
    10081 gtgctggtcg gcgcactgac cggccgctgg gcgcagcccg atgacctcgt caccggcctg
    10141 atcttcctcg ccgtgggcat gggcgtcgcc ttgagcggcc tgggcgtggc cgaagtcacg
    10201 tcctgtgtgc tgatgtaccc ggtcgcctca atggacaagc cgttctccgc gcctcaaggc
    10261 cgcgccatgg cccaaggctt cttcccgttc gtctacgtgc ttggcagcct gctgctgatg
    10321 ctgcccaccg gcatcgtggc gctggtcatg ctgctgacgg gagccgaagg cggcatatgg
    10381 ctggcgatta ttccggtcgc actggccaac ggcatcggca tcctggcact cgggacatgg
    10441 ctgggcggta agctgatgga tgcgcgaatg ctttcgattg tggcagcact cgactcgttc
    10501 gcctcattgc agaaatgatc gccttggcat aaggagcacg atggtatcta cagcggtatc
    10561 acgccgtcag cggcgtgaag cgcaacggcg gacccaacga tcgcatcgcc gaacactgac
    10621 cgtcatcatt tccgcactga ccaccatcgc cctgttcgcg gggtactgcg ccgccgacat
    10681 caccgacgtg gcccccggat tattgacgtt gaagcccgtc gccgcacctg tttttgccga
    10741 tccagccacc gccaaatcag gcggcaccgt cgccggcacc ttgaacgcga acaaggccat
    10801 tgattccacg gccgcttctg cgctggtcaa cgaattgctg tccgcgcaag gcgtgggaaa
    10861 cgatacgtcg gtgatcattg aagacgccca aggcaccgtc gctgccgaac acgagtcgaa
    10921 cactccacgc gagcctgcat ccacactgaa aacgctgacc gcgctcgccg cctcatccac
    10981 cctgaatatg gcatccacct tggataccca agtgttcctc acccaatccg atgacggcac
    11041 caatacgttg accctcaaag gcaatggcga catgctgctg tctgcaggcg attctgacgc
    11101 caaccatacc aacggacgtg ccggtctgaa caccttggcc aaggccaccg tggccgctct
    11161 cgcccagcga ggcatcactt ccgtcaatct cgagtacgac gacaccctat tcggcgactc
    11221 acgcattccg gcaggtctgt cggaaggcgg cgcagtctta agcgactaca ccgtctactt
    11281 cacgccggtc tcatcaatgg cgattgatgg aggccgccaa tacaccgccg atacgcctgc
    11341 gccggccgac cccgatgatt cggccggata cccggaactg tcccagcacg ccgccagcga
    11401 cgtggccacg aaattcgccg aactgctcca atcgaacggc gtggccgtta ccggagacgt
    11461 gacggcaaac accgctccat ccggtgaaac gcctttggct tcggtgagtt cggcaacact
    11521 gtccgaaatc atggcataca cgttgcggca ttcggacaat acgctggccg aggagttcgg
    11581 cagactcacc gccttggcga aatcagcgac aaacagtcct gaaggcggca cggaagcggt
    11641 caaatccact ctgaacgatc tcggtctcga cacatcc
//
LOCUS       DAWBMW010000031        11589 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_1404_length_11589_cov_3.211722, whole genome shotgun sequence.
ACCESSION   DAWBMW010000031 DAWBMW010000000
VERSION     DAWBMW010000031.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 11589)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11589)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11589
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1404_length_11589_cov_3.211722"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    10366..10375
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2448 a         3737 c         3390 g         2004 t
ORIGIN      
        1 cccattacca tgtttggtag gtcccgatgg tcggggccgg gtcgccggtt ccggtatgac
       61 tttcctgccc tgtcgttgat gatccggggg gtgttgtcgc cgggaacggg gacgcccgat
      121 gaccgctgat aggaaccagg ccggctacac ggacgcttca ggggcgcatg gcgcaggtct
      181 cgtcgtaatg tgggtgcggc gcgggcgtcc ggactcctcc gagcccgcgg cggccggaaa
      241 accttcatga cgggagagac gatgatgact ccggacgaca tcgacgtgtg ggtcggcctg
      301 gacgtgggca agagcgcgca ccacgcgcac gccctcgacc atgacgggaa caccctgtac
      361 gacaagccgt tgaaacagga cgagaaggcc atccgcacga tgctgggcaa gctctccaag
      421 catggccgtg tgctgctggt cgtggaccag cccaacacca tcggctccct gcccctgacg
      481 gtggcgcgga acatggggat cgcggttgcc taccttcccg gcacggccat gcgcaggacc
      541 gcgcagctat tgcccgggga cacgaagacc gaccgcaggg acgcgcatgt gatcgcgtgg
      601 gccgcgctca agctacccga gacgctcagg gacgcgggcc cggatgacga gaccctcgcc
      661 gccctgaagc tgctcgccgg ccacgacgag gacctcgccc acgaatccac ccgccacgtc
      721 aaccgcctgc ggagcctgct gctgcagacc caccccgcgt tcgaacgcgc gctcaagggc
      781 gagagaatca cgcgcgacgc gacgctcgcg ctgctcgaac gatacggcgg gcccatgggc
      841 gtgaaacggg ccgggatcga ggacgtgaag gactgggcca agtccaacgg gcttcgcgcc
      901 ggccgcatca tcgacgacat gttcaaggcg atcggcgagc agaccgtcac cgtgcccggc
      961 accctcatgg ccgagaccat catcccctcc atcgcccacg acatcaagac gatcagggac
     1021 cggcggcgcg aggtcggccg ccaggtcgag aagctcctgg aggaccaccc tcttctcacg
     1081 gtcctgacgt cgatgcccgg gataggcgtc aggaccgcaa gcaacatcct gctcggcatc
     1141 ggcggcgaca tcgccaactt caaatcgtcc gcccatctgg ccgcctacgc gggcatatcg
     1201 cccgtcaccg gccaatccgg caccagcatc aaaggcgaac ggccctccag gcggggcaac
     1261 aaacgcctca agaacgcgct gtggcagacc gcgttcgtcg catccaccaa acacccgccc
     1321 tccgtggcct attacaagcg caaacgcgaa cagggcaaac accacaacgc cgccatcatc
     1381 tgcctcgcgc gtcggcgctg cgacgtgatc tacagcatgc tcaaaaacgg aaccctctac
     1441 caggaaccag ccctcgtagc ctgaaccggg ccacacggga aaggacgccg gacgccaccg
     1501 gcagggcgaa gcgcgcccac gacagccaac cggaatcccg acacgtcaac tccccaagga
     1561 gttgacaaaa acataggaac acccccccaa aaaaacaagg gggcaccctc agctggaacc
     1621 acgtccggcg aaccgggacg cagttcagcc cgaggggacg cggccaatac agccccggga
     1681 cacagccaca gggggtgcaa acacgggggt gcgaccgggg ttgcatctcc ccgcctcggc
     1741 ggcgaccaca ggagtgcggc caacacagac agaggggcac agacagaggg aagagtagcc
     1801 aatgtaacca gagggatgcg gccagtgaga tacggtcaga ggactttggt ctctgtggtc
     1861 gaaccgttaa gcggacagcc cagtgggctg tccgtaggta aggggagcag ctatgctgcg
     1921 acggattcct atggagacga aagttctcgt cagagaacct gagtctccgt aggaatcacg
     1981 ggctcgccct tcatcaggct ttgtgcggtg atgggcagtt cggacaacgc cttggcgcgg
     2041 tagtcgtggg cggccatgat ggcgtcatgg ccctgccact gcgcatcgat atggccgtgg
     2101 tccacgaaat cgaccagcag gttcttccac gtcggttcgg gcgtgaaacc ggccagcttc
     2161 tcttccgagc cggaaatcac gtgttcggct tcggctagcg cgtattcgta ggagcggaat
     2221 gccaacttgc gaccgggcac tgtggccttg tccttggact tcttcatgac cggaaccatc
     2281 gtgccgtcag cgccttcacg ctcggtgagc ttgtacacca tcgcacaagt gggagcgccg
     2341 gagccggtga ccagcatggt gcccacgcca taggagtcga ccggcgcggt ctgcaatgcg
     2401 gcgagcgcgt actcatccaa atcgttggtc acggtaatgg tggtgttcgt ggcacccagg
     2461 gcgtccagct ggttgcgcac acgctgtgcc atggcggcta ggtcgccgga gtcgatgcgc
     2521 acgccaccca gttcgggacc ggccacttcg acggcggttt tcacggcttc ctcgatgttg
     2581 taggtgtcca ccagcagcgt ggtgttcttg cccaatgcct cgatctgcga ttcaaaggcg
     2641 tcacgctcgg aatcatgcac caacgtgaag caatgcgcgg cggtgccgat ggccttcaga
     2701 ccatacagct gggcggccag cagattggcg gtacccttga atccgcccac tacggcagct
     2761 cgagcggcgg ccacggcggc ccactcgtta gtgcggcgtc cgcccatatc catgcaagga
     2821 cgatccttgg ctgccgacgc catacgtgag gcggcggagg ccactgcgga atcgtagttc
     2881 agcacggata gaatcaacgt ctccagtagc gtgcattcgc cgaacgtgcc ttcgacctgc
     2941 aaaatcgggg agttcgggaa gaacatctca ccttcgcggt aaccgcgaat cgtaccggaa
     3001 aagcggaagt tctccagcca cttaatggtc tcggggctta ccacgtgacg gtcgaccagg
     3061 aacttcagat ccgcttcatc caagtggaaa tgttccagcg catccagaat gcggccggtg
     3121 ccggccacca cgccatagcg acggccttcg ggcaagtgac gcgtgaacac ctcgaacacg
     3181 cacttgcggt cggcggtacc gtctttcaga gcggcgtcga gcatcgtgta ttcatacatg
     3241 tcggtcatca gagccggcga ataatcgacc attgcaaggc ctttcctaca taagtggtga
     3301 agttgcacct aagtgtagtc cgggcaaagc cgtgcgggaa gggctcgtct tattctcgta
     3361 tcgccagatt cgatattgcc gggttcagta tcgtcatatt catcggcact atggcggtcg
     3421 gtcacaccga ccataatcat cacgaccagc caatacgcca gccgcaccat acggcccact
     3481 ggcgaagcgg tagactcgat gcatgagatt catcgtggcg ttgtggcgct ttgcgatcgc
     3541 cggtttctgc ttcgtgggca catatgaggc atggtccaaa ccgcagttct gggtgtattt
     3601 cacgttccag accggtttcg tgctgggcat cgtgatgctg tggtccggcg cggccacact
     3661 gctcaaaggt attcagccgc ctgcatggct taaggggtgc ctgactgtgt acgccatagt
     3721 caccgcactg gtggcgttct tcctgatgcc tccggacgac ccggcatacg tgccacaggt
     3781 tattggcatc atgaccaata cgatgctgca caaaatcgcc ccgattatgg cggttatcga
     3841 tttcgtgcta ttcgacccgc accggcgttt ccgctggcat tacatgttct cgtggctggc
     3901 gtacttccct gcatacttgg tcttcgtgct ggcccgtgcg gcgatttggc cggggtccgg
     3961 accggcggcc ggcggcagtc cgtatccgta cgacttcatc aaccttgacg cactcggctg
     4021 gcaagggttc ggcattagct gcggcaagct ggccatagcg ttcttcgtga tatcgctcgt
     4081 tgtctggctg ctcgaccgcg cactgccgag caaggcctta ctcggataga cgcgcccatg
     4141 atcgacgcac atcggtcttg gtgcccagcc ggtatccaac acagcactcg atggcctcaa
     4201 cattcgatgg cctcagcact cgatggactc aacattcaat ggaataggcg cggtttttgt
     4261 actcgcgcca atccggccag tcttcaccgg cgtcgatggc acgcttgatg tcgccatagt
     4321 aatggagctt gcgctgcacg tgcatggccg cctcgttgag ggctcggcgc tgctcgtcca
     4381 aggattcgcg gcgagattcc agcagatcaa ggatttcgtc gatatgctcg ggctcattgg
     4441 actcatacac gaagaacttc ttaagatcgt caatggtcat gccggcgttc ttgaagcagc
     4501 aaatcgcgcg cagacggtcg atatggcagt cttcgtatat acgctggccg gtttcggtgc
     4561 gctccacgtt ggtcagcaat ccctgatcct cgtaatagcg cagcgtagag ggctgcatat
     4621 ggaattgcgt ggccacctga cgaatggtgt aggacatcat gactccttac tgcggcaaca
     4681 cgccgaagcg tcgcagccgt ttgcattcaa gttcacttga actggaacca tagtatccaa
     4741 gaacacttac gaataccgct caatcagcgt cggtttgccc atcgcaggta agcattcgta
     4801 agaacaagaa tgacattgta aggagactca atgttcgagc caaccgccat ctatgccccc
     4861 aacccgcacc gttacgacac gatgacctac aaccgtgccg gcacctccgg cctcaagttg
     4921 ccggccgtct cacttggctt ctggcacaac ttcggcgaca tcacgccatt cgaccagatg
     4981 aagtccctgg tcttcaccgc cttcgacaac ggcatcaccc acttcgacct agccaacaac
     5041 tacggccccg agcccggcca ggcagagaag aactgcggac tgctgctcgc caagtacttc
     5101 aagcatcacc gcgacgaact cgtcatctcc accaaggccg gctacgaaat gtggacaggc
     5161 ccctatggcg acctcggcag ccgcaagtac ctgctcgcca gcctcgacca gtcgctggaa
     5221 cgcttgggct tggattatgt ggacatcttc taccaccacc gcccggatcc ggaaaccccg
     5281 cttgaggaga cgatgggcgc gctggctcag gccgtgaact ccggcaaggc cctgtacgtg
     5341 ggtctgtcca actacgatgg tccgacgatg gaaaaggccg ccgcaattct ggcagaactg
     5401 cacgtgccgt tcatcatcaa ccagaacaag tacaacatcc tcgaccgcac cgttgaaaag
     5461 aacggcctga aagagaccgc cttcaagctg ggcaaaggcc ttatcacctt ctgcccgctg
     5521 gctcagggcc tgctgaccaa ccgctatctc aacggcattc cggccgacag ccgcatggct
     5581 cacgatccgc gattcctcaa cgattccgct ctgaccgaga aactgcacaa gcaggtcatg
     5641 gatctgaaca atctggccgc cgaacgcggc cagacgctgg ccgagatgag cctggcatgg
     5701 ctgctgcacg acggcaaggt gacctccgtg ctgaccggag cctccaagcc gcagcagatt
     5761 ctcgacaaca tcggcgcgct gaagaacacc cacttcagcg acgaggagct caagctcatc
     5821 gacgagattt cggctcggtg accattttgg ctcccctcgc tgaggggagc caagtaccac
     5881 ctttagcgcg tcatttcctc ataaatccgc ttgcagtcgg ggcaaaccgg gtacttggac
     5941 gggtcgtgct tggggaccca aaccttaccg cacagggcga ccaccggacg accggtcgca
     6001 cgcgattcag cgatacggtc cttggatacg tagtgcgcaa aacgatcggc gtcgccacca
     6061 tcgtcacgaa ccggcgactc cttggtctcc ggacgctcca gcaccgacgt gccggcacct
     6121 tggtccgggt tgcgcagcgg ttcggcatca tcaacgaaca tctcggcaaa agtcatggtg
     6181 cacttctttc ctcgcattca acgattcgac tgcactccat catagattcc cgtcgttacc
     6241 cgcgaaaccc agtggcgcgt aatccgcaca cctccacaca cccatgtcgc gcatcacgcc
     6301 accccgccac atacaatgcc acatacaatg ccgcacacga cgccacaccg acgccacata
     6361 cggcaccgca cacactgcct catacgccga cgcgacaaca atgcacgctc ggcagcacga
     6421 agcacaccac tagcggtagg ctataaagca tgactatcgc agtatgcccc ggctcgtatg
     6481 accccgttac cgccggacac ttggatgtaa tcgagcgctc ggcgcggttt ttcgacgagg
     6541 tgcatgtggt ggtggccgtc aacgccgcga aaacaccgat gttttccgaa gccaccagag
     6601 tcgacgtgat ccgccgcgcc cttgacaaag ccggttgcaa gaacgtcacc gtttcttcca
     6661 ccgacggcct gatcaccgac tactgcaaga aggtcggcgc caccgttatc atcaagggtc
     6721 tgcgccagaa cggcgactat gaagccgagc tcggcatggc gctggtcaat cgcaagctcg
     6781 ctggcatcga aaccctgttt ttgccggccg atccgattct tgaacacatc tccagttcca
     6841 tcgtcaagga cgtggcccgc catggaggcg acgtgaccgg catggtgccc gattgcgtcg
     6901 tgccgatgct ggccgatgcc ctggccgagg aacgtcagcg gaaggactga tgcactaatg
     6961 actatgagcg atgaccaccc tactggatac gtcgatctga ccaaaccgaa tgcggacgcc
     7021 gcatcgtcca ctccggagcc gtccgccccg gaaccgcccg cgtctacgca agccgcgccg
     7081 gcacccaccg aagaggccga tgttctcgcc ggatcgccgc ccgccgcgcc tacccctact
     7141 ccggcacaga tgcaggccaa agcgcaggcc caagcagctc agcagacccg ggcacaggct
     7201 cagaccgctc aggccgccgg cattcgcccc tcgttcatga acgaaatgcc cgacctgcga
     7261 gaaaccacgg acgacgaccc ggccacggtc aagagccacg aggaattcac cacggtgtat
     7321 gacatcatcg accagctcga ggcggcactg ggcgaagcga agtccagcat attcgccccc
     7381 ggcatggtga aggttgaccg cgacgaattc accgaccagc tttccgagtt gaagaaaatg
     7441 ctgccggtgc agttggagcg cgcctccgcc ctcatgcgcg aggccgagcg ccgtctggaa
     7501 tcggcacaga cccagtccaa cgccatcgtc gcctccgcac aaagccgcgc agccaacatg
     7561 atcaaggacg ccaacgaaca ggcacagttc ctggccggcc aagagaacgt caccgagctt
     7621 gcacggcaga aagcgcgcgc gattcttgac caggcacaga ccaaagccga ccatctgacc
     7681 caaggcgcgg accagtactg cactacggtg atggaagggc tgtcccagca gctcggcaaa
     7741 ctcagtcagg atgtgcaggc cggtcttaac gtgctggaag agcgccagcg cgcggcccgc
     7801 gaacagatgc cgcacctgac gctgaacgac tatcccgaag cccagtaacg ccagcaacgc
     7861 cggcaacatc agtaacgcga ataacgccag taacgaataa ctccagccaa catacgaaag
     7921 gcaccaagaa cataccatgg cacgagttga agattccccg tgggccattc cggtagcgca
     7981 gattgcttcg cgcgccggtc aatccaagcc gattgacgcc gatttcccgg caccttccgg
     8041 cattggcgac agcattgtcg gcatcaagga aggtgagccc gtacacgtga gcgggcagtt
     8101 cgattcgatt gtggacggcc tgatcttcac cggccgcctg gtcgcgccat tcgtcagcga
     8161 gtgcacgcgc tgcctgaagc cgattgacga ggactggccg gttgatgtga cggtgttctt
     8221 cccgtacgag tcgggtcagg acaaggccaa cggcaagggc ggcaagagca agaaggacga
     8281 cgaaatcgac atcatcgccg gcgaggacga gtcggaagac acgtatccgc tgctggagaa
     8341 cggcgcattc gcggacatcg aggcgatgat ccgcgacacg ctggtcgaat cgctgccgtt
     8401 gcaaccactg tgccgaccgg attgcagggg tctgtgctcg cagtgcggtg ccgatctgaa
     8461 cgaggatccc gaccatcatc acgatgtgac ggatatccgt tttgccggat tggcggggct
     8521 caaagcccag ctcgaggccg agcagaacgg cgagcaggcc gagtgacgcg cgcctgaacg
     8581 aagtttgtac gagccgtgcg ttaagataac ggacgcttaa gctcaattgt tcgaacgaaa
     8641 tagaggatac tcaaatggca ctgcctaagt acaagacctc gcgcgccaac acgcattcgc
     8701 gtcgcgcgaa ctggaaggcc accgctgctg cgaccgtgaa ctgcccgaac tgtggcgctc
     8761 cggccctgcc gcacatggct tgcccgagct gcggtaacta ccgcggccgc acctaccgct
     8821 ccgccatcca gccggctcac accaagtgag tcctgcgata acggcttgaa tgaaggccct
     8881 gccatcgacg ggtggcgggg tcttcgcata agtgcaccat tgcgtacacc atcgcatcgc
     8941 aaacaacaca acacaacaca acacatcacc atcgcgtgaa ggaccataca acaatcatga
     9001 gtaatgaaac cccacagcag ccgacgccgg ccaacgagct tcttgaagcg ttgggcacca
     9061 ctctcagccc ggatctgctc gtgcaggcac tcacccaccg ttccttctcg catgagcacc
     9121 ccggcgtggc caattacgaa cgcctcgaat tcctgggaga cgcggtgctc gaactggtct
     9181 ccactgaaac gctgttcacc attcacccgg atatgaccga ggggcaactg gccaagatgc
     9241 gcgccaaggc cgtctccgaa gacgcgctga gcgccatcgc caaaaccaag ctcaaggtgg
     9301 gcccctacat tctgctgggt cacggagaag ccgagcaggg cggcgcggag aagaactcca
     9361 tcctatgcga catcgtggaa tcgctcatcg gcgcgacctt cctggaacac ggcatcgacg
     9421 aggcacgcaa ggtgattcac cggcttattg acgacacgct cgccgaagtg gccaccgagg
     9481 gcccggcact tgactggaag acctcgctga ccgtcaaggc gcacggcctc ggcaaggagg
     9541 agcctgtcta ccatatggag gtctccggcc cggaatacgc gcagatcttc accgcgcgcg
     9601 tctcattggg cgagaacggc gacatcatcg gcatcggtaa gggctccagc aagcgtaagg
     9661 cgcagctggc ggccgccgaa gcgggctgga agtcactgga ctcgttcaag acgcggacta
     9721 agtaagcccg ctgtcggatt tgcgaataga ctaataccaa catgagcgac gagtcgcgcc
     9781 ggggccacaa gtctcgggga agcgactgga atcagacacg cgggcacccg gccccatccc
     9841 aaggtcgggc aggttcgcga caaaacctat gaggtgagag gaatcatggc tttacccacg
     9901 cctttgcagg cattcagcgg tgtaccgaaa atcaacacgg cccccgataa gcagaccatc
     9961 gtcgatggcg agaagatgac tggcgcccag gcgctgattc gttcgctgga ggacctcggc
    10021 gtcgaggacg tcttcggcat tcctggcggc gcgattctgc ccgtctacca cgagatcaaa
    10081 gacaacacga agttccgctt cgtgcttatg cgccacgagc aggccgccgg ccacgccgcc
    10141 gaaggctacg cactggccac tggcaaggtg ggcgtgtgca tcgtgacttc cggcccgggt
    10201 gccacaaacg tggtcacggc cattgcggac gcgaacatgg actccgtgcc catggtcgtg
    10261 attaccggtc aggtgggcgt gcaagccatc ggcactgatg ccttccagga ggcggatatc
    10321 gtgggcatca cctacccggt gtccaagcac tccttcctcg tgaccnnnnn nnnnntgacc
    10381 cgcgcgcagg acattcctcg cgtgctttcc gaggcgtact acatcgccaa caccggccgc
    10441 cccggcccgg tggtcgtgga tctgaccaag accgcacaga ccggcgacat gtactactca
    10501 tggccgcagc gcatgattct gcccggctac aaccccacca ccaaggcgca cggccgtgtg
    10561 ctgtccgacg cggcgaagct gttctctcag tcgtaccgcc cggtgctgta tgtgggtggc
    10621 ggcgccgccc gctccaacgc cggcgcacag gtcaaggctc tggccgacct gaccggcgct
    10681 cccgtggtca cgaccctgcc cgcacgcggc atcattcccg actccgaccc gaagaacctc
    10741 ggcatgctag gcatgcacgg tacgattgcc gcaaccggcg ccgtgcagcg cgctgacctg
    10801 cttgtggcca tcggtgcacg tttcgacgac cgcgtgaccg gcaagctcga cgccttcgca
    10861 ccgaccgccc gcgtgattca cattgacatc gacccggctg aaatcggcaa gaaccgtcag
    10921 cccgacgtgc cgatcgtggg cgatgtggct accgtgctcg acgacttgat tcccgagatt
    10981 cagcgtacgc aggccattca gggcaagccg aatctggccc cctggtggaa ggcgatcgac
    11041 ggctggcgcg aggaataccc gatgacgtgg gatgagccga ccgacggatc gctggccccg
    11101 cagtgggtca tcaagaagct ctctgagatg gccgatccgt ccaccatctg ggtgaccggt
    11161 gtgggccagc accagatgtg ggcctcgcag ttcatcgatt ttgagaacca gcacgcttgg
    11221 atctcctccg gaggcctcgg cacgatgggc tacggcctgc cagccgccat cggcgcatcc
    11281 gtgggctcgg cccgcgagtt cgagggcaag aagccggtgt ggctgatcga tggtgacggt
    11341 tccttccaga tgacctctga ggagctggcc gccgcgttcc tggaccacgc tccggtgaag
    11401 atcgcgattc tgaacaactc cgtatacggc atggtccgtc agtggcagac gctgttctac
    11461 gagcaccact actcgcagac caacctgctc gatggcgagg cgcatggcgt cgacggcgag
    11521 tcggcactgg tcgacggcga tgcgccgctc gaagtgcctg atttcatcaa gttggccgag
    11581 gcatacggc
//
LOCUS       DAWBMW010000032        11546 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_1409_length_11546_cov_2.643982, whole genome shotgun sequence.
ACCESSION   DAWBMW010000032 DAWBMW010000000
VERSION     DAWBMW010000032.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 11546)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11546)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11546
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1409_length_11546_cov_2.643982"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    8879..8888
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2491 a         3399 c         3363 g         2283 t
ORIGIN      
        1 accttggcca cattggcggc cggcacggtc agcaccaggg cgatacggtc cgcgatgtcc
       61 agatcggcag ccttacgtgc gtcctgcaca gaacgaatca cgtcgcgggc gtagccttca
      121 gccagcaggt cgtcatccag cgcggtgtcg aggatcacga agccaccggt cggcagagcg
      181 gcggagaccg aagcggcggc cacggtggcg ttctcctcct ccacacggtt gatcagctcg
      241 tactcgcctt cgaccagcgc aaggtcgccg gacggcgtgg agaccaccgg cgcaccggac
      301 gcagcatcga catgccagtc gccggacttg gaggccttga tggcgaactg cacctgcttg
      361 ccgaggcgcg gacccgcaac acgggcgttc acacgcagct cgtgcacgat cttcagaccg
      421 tgggcggcgg cgtcttccag agtcgagaac tcaatgttct taatatttaa ttcagacttc
      481 agcaactcgt cgtatgcctt gacggcgtcc acgttctcga ccaccacggt gagcttggaa
      541 agcggctggc gcacgcggat cttggcggcc ttgcgcaggg acagggtgcc ggaaacaacc
      601 tcgcgcacct tctccatcgc atccaccaga gccgggtcgt cgaccagcac gcggccgagt
      661 tcggtgtctt caccggtctt ctcgtccacc acgaacggcc aatcggccag gtgcacggac
      721 tcgccgccgg tcaggccgcg ccacacggac tcggcttcca tcggggccag cggcgcaagc
      781 acgcgcatga acgcttcaag cacggtgtac agcgtgttga acgcggacgc atcctcgttc
      841 cagaagcggt cacgggtgtt gcggatgtac cagttggtga gcacgtcgat gaagtcggag
      901 accgcatcgc aggcgtcgga aatcgcgaac tcgttcagcg acttctcggc ggccagcacc
      961 aggcggcggg tacgggccag caggtagcgg tccatctccg gcagaccggc gacctcgtcc
     1021 gcgcgcagct ggcgggcgtc gaatccggcc ccgccgttgg ccgcgttggc gtacaaggtg
     1081 aagaagtagt aggagctcca caccggcaac atgacctggc gcacggtgtc gcggatgcca
     1141 tcggcggtga cgatcaggtt gccgccgcgc agaatcggcg aagacataag gaaccagcgc
     1201 atggcgtcgg aaccgtactt gtcaaacacg ccgttcacgt ccgggtagtt gcgcaagtgc
     1261 ttagacatct tctggccgtc ggagccgagc acgatgccgt ggcagatcac gttcttgaac
     1321 gccgggcggt cgaacagggc ggtcgccatg acgtgcagca ggtagaacca gccgcgggtc
     1381 tggccgatgt attccacgat gtagtcggcc gggaagtgct gctcgaactt ctccttgttc
     1441 tcgaacgggt agtggaactg cgcgaacgac atggaaccgg actcgaacca gcagtcgagt
     1501 acatcggaaa tacggtgcat gtgcgacttg ccggtcgggt cgtccgggtt gacgcggacc
     1561 aggttgtcga tccacggacg gtgcatgttg atgttgccgt ccttgtcgcg cgggtagtca
     1621 ccgaagtcgg ccttgagctc ttcgagagaa ccgtagacgt ccacacgcgg gtacttcgga
     1681 tcgtcgctca cccacaccgg gatcggggaa ccccagaagc ggttgcggga gatggaccag
     1741 tcgcgcgcgt tggcgagcca cttgccgaac tgaccgtcct tgacattgcc cgggatccag
     1801 ttgatctgct ggttcaattc caatagacgc ggcttgatct tggtcacgga cacgaaccag
     1861 gaggagaccg gcttgtagat cagcggggtg gcgcagcgcc agcagtgcgg gtaggagtgc
     1921 acgtagcttt tctcctggaa gaggatcgcg cggcgttctt ccggaatctc ggcgagcggg
     1981 ccgtcgccgg cgcgcaggtt gcgcaggatc ggcaggttgg cgtcgaacac gaaatcgccc
     2041 tcgtagtcag ggcactgcgc ggtgaagcgg cagccatcgt cgagcacgtc ggtgctcttg
     2101 atgccgtgcg cgttcagcgt attcatatcg tcctcaccgt agggagcctg gtgaaccagg
     2161 ccggtacctt cgacggtgtc gacgtagtcg gcggtgaaga tcgtgtagcc gttcgggccg
     2221 ggcacgtggc cttcggattc ggcctcgtca cccgcgaagt acgggaagac cgggtagtag
     2281 cgtcggcctg ccagctcgtt gcccttgagt tcgcgcacga cctcgtaatt ctcgccgagt
     2341 tccttctcat agtgcggcag caaatccttg ccgaggtaga acttcttgcc agcgaacttg
     2401 ccttcggtcg gacgaacttc gacgtagtcg atgtcggcgc cgaccacgat ggcgaagttg
     2461 gtgggcacgg tccacggggt ggtggtccag aagacggcgt aggcgtcgtc ctcatcgcgc
     2521 atcttcacgg ccacggacac ggtggtgtcc tggcggtcct ggtacacgtc ggcgtccatg
     2581 cgcagctcgt gcgcactcaa cggcgtgcgg tccttcgggc agtacggcag cacgcggtag
     2641 ccttggtagg ccaggccctt gtcgtagagc tgcttgaagg cccacatcac ggattccatg
     2701 tacgggatgt tcagggtctt gtagccgtgc tcgaagtcca cccaacgggc ctgacgatgc
     2761 acgtagttct gccattcgtt ggtgtacttc agcacggaag cgcggcaggc gtcgttgaac
     2821 ttgtcgatgc ccatcttctc gatctggtcg accgagtcga tgccgagttc cttctgcgcc
     2881 tcaagctcgg cgggcaggcc gtgggtgtcc cagccgaaca cgcggttgac cttgcggccc
     2941 ttcatggtct ggtagcgcgg gatcacgtcc ttggcgtagc cggtcagcag gtggccgtag
     3001 tgcggcagac cgttcgcaaa aggcgggcca tcgaagaaca cgaattcgtt ctggctgtgg
     3061 tcgccggaag ggttgcgttc aacggacttc tggaaggtgt cgtccttgtc ccagtagtcg
     3121 agaacggtct cctccatgtc ggggaagctg gggttcggcg cgacgtgcgc ggtctcgccg
     3181 ccctcgttcg ccttgggata tacgttttcg ctcaccgtaa atctcctgtt agtactgtgc
     3241 actggtccta caggagcgtc attgatggcg attgccgggc gccgacgtgt gccggctggg
     3301 ttcacgaatg tcacgtaccg aaccgtgggg ccgatcgcgt caatcgcctc gcagccggaa
     3361 ctgactgtgt tccgcccgga acagccctgt ctgccgttcc tgcctacgag gacgatgacg
     3421 ggcgctggta tgcgctccgc accgcggtac cacctcgctt atcgcaccgg ttgcgccgct
     3481 ccgaactgtt gccctcggag ccgcgagcct gcgcgaccgc ttgtgttcgg ctgtctcggg
     3541 ccgatcccgt cggttctact gggcgattgc cgccgttctt ccgaagactc cccgctgata
     3601 acggatcact gcctcacaac agtgcatatc ctaacacgcc acgcggtcaa atcgccgccc
     3661 acggccacgg caccgtcagg agcccgattt agattgggac cggccaccac ggaccgagaa
     3721 aggattgtcg gacaatgacg tatgatttca cgtccatcat gaacaggcat ggcatggatt
     3781 cgatcgcggt cgacggactg ggcaccgacc cgggattcgc gccggacccg ccacgcgagg
     3841 gtttcgacgt catccccatg tgggtggcgg acatgaactt cccgaccgtg cccacgatcc
     3901 cgcaggccat catcgaacgg gcgaagcatc ccgcctacgg gtacttctcc cccaccgacg
     3961 actattacag ctccatcatc gactggcagt ccacgcgcaa cggggtcacc ggattgaccc
     4021 gggaggcgat cggctacgag aacggggtcc tgggcggtgt gatcagtgcg ctgaccgcgt
     4081 tcgccgcccc cggcgacggc gtgctgctgc acagcccgac ctacatcggg ttcacccgga
     4141 gcatcgaaaa caacggctac cgcatcgtcc acagtccgct cacgctggac gagcgcggcg
     4201 tatggcgcat ggattacgag gatatggaca ggaagctcaa ggagcatcac atccacgtgg
     4261 ccgtgttctg cagccctcac aatccttgcg ggcgcgtgtg ggaacgttgg gaaatcgaga
     4321 aggccatgga agtgtacaag gccaacgatt gcgtggtcat ttccgacgag atttggtccg
     4381 atttgactct ggggaaccac cagcacatcc ccacccaatc ggtcagcgag gacgcacgca
     4441 acaggaccgt ggcgttctac gcgcccagca agaccttcaa tctcgccgga ctggtgggaa
     4501 gctaccacat catctacaat cccacactcc gggaccgcat cgtggccaaa agctcgaaat
     4561 gccactacaa cgacatgaac gtgctttcca tgcacgcgct gatcggcgcc taccggcccg
     4621 aaggccatga gtggctcgac gaactgaagg cggtgctcac cggcaacgtc gattacgcct
     4681 acgactacat caccaagcat ttccaaggag tgagtctggc caaaccggaa ggcacctaca
     4741 tgctgctgct cgactgcgag cagtggtgcg aacgccacgg cctgagcctg cccgagttgg
     4801 agaaacgcgg ctgggatgtg ggcgtcgcat ggcaggacgg cgacatgttc caggccccct
     4861 acagcatccg cgtcaatctc gcattgccct tgagccgcgt caaggaggcc atgcgccgcc
     4921 ttgaccaata cgtattcaac gcgtaaacgg catgcatccg cgattgcggt gtcctggaaa
     4981 gacgaatgcc gtcaacgagg ttggtgcccg ttggcggcat tcgtcatacg ttggccgaac
     5041 cgggcgaaac ccaagttgcc aagttgccgt gcgctatgag gtggatgccg ccagcggacg
     5101 tgtacatctg accgcccgat atgccggtgc caccgatgcg ccgacgctgc cggcattcgg
     5161 attggaatgg acacttccca aacagtacga gaacctgcgc ttctacggcc ttggccccga
     5221 ggaaacctat cacgaccgtc tgcacggcgg caagctcggc attttcgagc gcaccgcggc
     5281 cgaagacaat gcaccgtatc tggtgccgca ggagaccggc aaccatgaag acttgcgttg
     5341 ggccgaagtg cttgacgccc aagggcacgg catgcgtatc agccaggccg gcagcgaaca
     5401 tttggcggcg agcctgttgc cgtacagctc gctcatgctg gaagaggcga cccaccagaa
     5461 cgagttgccg ccggtccgtc acacgttcct gcgcctgttg gccgcgcaaa tgggcgtggg
     5521 cggagacgat tcatggggcg cgcccgtcca tgagcagtac cagctgcccg ccgatcgcgc
     5581 ctacacgctc gacgtgaatc tggaactgtt ctaacagcga taaagaagct ccctctgacg
     5641 agggagcttc tttatcatgc tggtacgaaa ctgtcacgca ccactagggt ggtggagatg
     5701 aaggtatggt ggtgcagcgt gcgccgactg gagatggcct cggcaagcat cgtaatcgca
     5761 gtatcggcca gctcattctg gtcgatatcg taactggtca atgtcggcga agtgtactgg
     5821 gcgatggcct ggttattgat actgatcaca ctggtatcgc gaggcaccag cactcccgca
     5881 gcggtaaacg cctgcagtac gccgactgca atcgagtccg cagccacgat gaacgcatcc
     5941 ggcatgtcat ctcgatgatc gttaaccgct tcctccccta gagtgcgccc ggtgtccaca
     6001 gtgaacgcgc cttgcgcgta aatcaggcct tccgcatcca gccccaaacg ctcggtccaa
     6061 ttgcggaatg cggttaatcg gccgtcttcc ggatattcgt gcaaacccat cgtgaagcca
     6121 gtaccgccga tatagccgat gcgtcgtttg cccgaggaca ccaacatatc caatgcgtcc
     6181 aaaatggttt ggcttaaatc cggttgtacc gaatcgaaca ggctgggcgc aggattgatg
     6241 tcgatgaata cgccataggg caataccaga tggagcttca acagtctatc cgaagagagt
     6301 acggacggtc cgatggagat gaagccgtcg aaatcaccgg cgtcggcaat cagatcgtcg
     6361 atatgggaaa acgacgtgag ttccatgtgc tgggctctgg cattgtcgcc gagcacggac
     6421 cgtagtttat cgaaataggc gtccgccagt tcttcctcgc gggtggcgtt gtcgagtacg
     6481 gccacacggc gtggcaatgc gatacggcgt gatccggtag cgtagccaag ctgctcgctg
     6541 acctcgatga tgcgacgtcg cgtctcctct ttgacagaca atgtcgggtc cccattgagc
     6601 agccgggata ccgtggcctg cgagaagcct gatttctgtg cgatttcctt catcgtgacc
     6661 atagttgagc cttccacgcc tcagtacgta acaatacgca gtatttcagt aaataacttt
     6721 actacatagt gtaactcacg tcatgcgggt atgacgccgt atggttgcag catcgtcgat
     6781 ccgcagcgct gatgagaacg tgcatgtttc aggccctccg ataattatcg ggcaggcggg
     6841 aatcccccga ccgtggagtc aatgcgctgg attttcccgg acttgtcaaa gacaacggtg
     6901 atgtccggcg cagtaaaact gccggtcgaa tccccgaatc ggtacggatc actccagtcg
     6961 ataacggcat gcatcgtcac ggtccgagtt ttcgcgtcat aggactcatt cgagtactcg
     7021 atactgttat gcgtggccgt agtcccgccc agaccgccgc cattgccttg atgcatttgc
     7081 tgacaatact tcgtaaaatc cgcgccgaga acattcgaca ctgcatagcg cctaaatgaa
     7141 cgtgttaggt ctgcaccaat tcacggccgg caatactcca cacttctcag taatcaattc
     7201 gtaccaaacc gctcacgaat ctgagcggca gtaccataag aggcttgcgc accgacccca
     7261 gtcgcggcgt acgcggaaac gtacgaggcc aactgcgcac tgtccgccaa cgcatgaccc
     7321 gaggcaagtc cggccagaac cgtgcccata aaggcatctc cgcacccggt agtgtcgatg
     7381 gccgcaaccg caactggcgc aatacgatag atatcatcac catcgatgac caccgaccca
     7441 tccccgccaa gcgtaattac cgcctgatta aagtcgaagt catgcatctg ccgcgccaca
     7501 tcagaccagt ccagtccatc ccaatcgtca tcgtcaggct cggcgatgtc cagcagctgt
     7561 gccatttcat gttcattgac cagcagaatg tccgaagcct cgataagttc agcgggtagt
     7621 tcagcaacga atggcgaatc gttgagcaat accttgagcc cggcatcatg acagaggcga
     7681 gcgcatgccg tgaccgtttc catcggactt tccaaacaca gtcccagtac atcggcggca
     7741 accaatgcgt cagatgactg gcgcacatag tcgacagaca cctcaccatt ggaaccagcc
     7801 gaatatacga tggtgttctc gccattcgcg tccacggtaa ttaccgtagt gccactggga
     7861 cctgcaacgg cagcgatatg cgaagtatcc acaccagcat cgcgcaaatg ctccagcaga
     7921 aaatccgcat tggtatcttc cccgactgca ccaaacatcc gcacctgcgc tccaattcgc
     7981 gcagccgccg atgcctgatt cgctgacttg cctcccggca ataaggtcag cgggccaccg
     8041 ttgacggtct ctcccggttt cggcaaccgc tcagtaacga ccgtgtaatc aacattcatt
     8101 gatccgacca cggacacact gccgtgcagg tggtcgagca agtcaagaca tgatgctgac
     8161 atgatgcatt tcctttcggc cttcttgttt cggcctgtct tctatgttct gcgcttgttc
     8221 tacgtttcag caaaatcacg atggaaacac aatcgtgcgg gtatgcgaat gcccggagtc
     8281 tgaagtcaaa tcccgggcat tgcacgtatt cggggccaaa tcgtctgcgc gattgatatg
     8341 ttctgctgac gactgacgcc tagcggattg tgatggttat atcagcatca cgcacccatc
     8401 ggcaggaaga ttgccatgga caggatgaac atgacgatta cgccagcgat catcggcaca
     8461 ctggtacggc gcacaatgtc gattgggctc ttcttgatgg agccggcgac gatcatcacc
     8521 acagcggaga ccggcgagac agcgcgcagc agattgccag cgagacccat cgggatggac
     8581 acagcgagca cattgatacc agcagcagcg gccagcggaa ccatgagcgg aaccatcgcg
     8641 aagaacagtg cggtaccgga accggagagt agcacgatga gcgcagtgag gccgacaagg
     8701 ataagcggca gcacgaagcc cataccagag ccggacaggc cggtcattgc gttctgcagt
     8761 tcggtgatca gaccaatgga cttaagacca acgacgaaca cagaagcagc gaccaacagt
     8821 gccacaatcg gcattgcgcc acccatgccc ttgaagaagg actcggtgcc agcgagggnn
     8881 nnnnnnnnga atggaagcga gttcaacact caggtcgatc tttgcgccgg tggtggaagt
     8941 gatggcgaac acgatgagaa gcatgatgat cggcagcagc ggcaaaatcg catacacagt
     9001 gcggtagaga gcgctacctt cgacagcctt aacctcagcc aggtcaacat caccgtcggt
     9061 caaatccgag ccagcgcgct tgtccatgaa cttctgccag aagtagtgtg ccagtgccat
     9121 gacgagcaag gtcgggatgg aaacgagcac gtgataacgg aagacgtaat cgctggcggt
     9181 caggccggag aacatgtcgg tcttagcgag ttcagcggcg atggcaacat tgtcggagcc
     9241 aagcggggtc ggcataatcg ttgcagtagt ggcaatgact gcagctgcgc tcagcaggct
     9301 cataccagct tggcgaagca ccgggtacag cgttgccata aggatgatag ccaggttgga
     9361 agcactcgga atgaccagag acaacaggtt gcccaacaag aaggtaatcg gcacgagtat
     9421 atacggggac ttgatgtgcg caatcggctt ggcgagcaca cttaccgtga cttcattggc
     9481 tccgatatgg gacatgtagc cggtgtagcc accaaggatg aggatgatga agccagcaga
     9541 agtgagcgtg ctcacgaact gatcgcctac aaccttgaac ggatcaagcc aagtgacacc
     9601 actggaaacg aagtcggtga atgagatgtc cttgcccatc agcaatgcga tgtacatcag
     9661 cacaatgccg atgaggaaca gggaaatctt gatgtccatt ttcttcacga gcataaagcc
     9721 cacaatgcca accgcggcaa ttgccacgag gtacataatc accagttcca tcggtctggt
     9781 cctttctttg ttctcttatt ctctgttgtt gaattcacga atactgatgc gctcaggggc
     9841 gccggtgtta gcggcgtcga agagacaagg gactatgcac aatgggcgat gccgtccatg
     9901 aaccagtcgc agaatcgatc tgcgtgaacg ccggtggtga cggtggcatt cggttcgcgc
     9961 ttaaggtagc ccttaagatc aacgaccgta caccctgcag tgagttcgcc agcgatttct
    10021 acgtccacaa atgccttagt catgtcgaat aggtcaggct ccagcaagca tgcgactgcg
    10081 caagcgtcat acattttgag accggtgccg aagctgcgct tgcggtagcg ctggaacagg
    10141 tcatgagcca tcagaccgac cttgcccata tccttaagct gagcggtttt ctccggcgga
    10201 atcacagttc ccaagccggc gtccagtgtg gccatagtaa tgtcaagacc agaagtgagc
    10261 acaatcttgg cggcttcagg gtcaacgaaa atattgaatt cggccataac acccttgttg
    10321 ccgcgagtga ccgaaccacc catgagcaca atgcgctcaa tacgcgactt gacttccgga
    10381 aaagttttta gcaacagtgc gatgttggtc agcggaccga ttggcatgac ggtgacgggt
    10441 tcagcagaag ccatgatctc gtcgtgcatt gcttcgacag catgcttatc cagcagaagt
    10501 tcagttttgg gctcagggaa atcgaatccc tccataccgg ttttaccgtg gatatcgctt
    10561 gcatcatcaa gctgacggat cagcggttca gcagcgccac gagcgaccgg tacatcctta
    10621 ccgtagtagg ccatcagctt gagcgcgttc gtcgttgtcg cttcaatgct gacattgcca
    10681 gcaaccgtag taatcagccg cacgtcgaag cgcgggttga acactgccag tgcgatggcg
    10741 acggcgtcat cgatgccggg gtcagtgtcg ataatcagag ggatctgctt cattgcttgt
    10801 tcggttcctt tcgcggtgtg ttgatatacg tgttatgcgt agtgctcggc gagccgatta
    10861 cttgattccg cttgtggcgg tggattcagc tgcgctgagc ggaacgggat caacgaattg
    10921 gactaatccg ctgccataac ccggtgccgg ccggcgcagg aacccatgct cttcgtagaa
    10981 cgattccttg ccaggtgttg ccatcaggcc aatgtacgca tggttgcagc agtgttctcg
    11041 aatgcgtgtg ataagtgcgc gcagcaccgc cgtaccgact ccaccccctt ggtattgcgg
    11101 taataccacg agatctttga tgaagaatgc gatacgtcca tcgccgacca cacgaccaat
    11161 tccggccacc tgaccatcaa cgtaggcaac cacggaatac agcccaccat ccagcgccat
    11221 acgcgcgtct tcgcggtcgt aatggtagaa acccacagcg tcgcgaacgc tgacgtacag
    11281 ttcggctgtc agcgcgtctt gctgaatcga tatgcacatt gcccgctcac ctcctctgtg
    11341 agtttcctcc aaaccgatca tcgttgatca tcgccattga taaaacgttt aatcaactaa
    11401 tgaaaattat gatataacct tttaccaatg ttgtcaacga tgtcggcacg tcagcactgt
    11461 tcagcgcata aacccagcct tcatgcacct gataaacgct gcaatatcag ggaacacgcc
    11521 acataggagc cccaaccgaa tcacgc
//
LOCUS       DAWBMW010000033        11532 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_1412_length_11532_cov_2.832535, whole genome shotgun sequence.
ACCESSION   DAWBMW010000033 DAWBMW010000000
VERSION     DAWBMW010000033.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 11532)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11532)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11532
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1412_length_11532_cov_2.832535"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6472..6481
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9854..9863
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11077..11086
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2215 a         3459 c         3570 g         2258 t
ORIGIN      
        1 gcacaccgca gtcgatgagc agcaggtgac cgttgtattc gatcacgttc atgttgcggc
       61 cgatttcacc caagccgccg agcggcgtga tgcgcatgga gcccttgcgg tacttcggcg
      121 gggcgatcag cacggcatcc tgctggggtg cggtggcctg ccgggtggcg ttgatcttac
      181 gggacggcgt acggctgttg gatccgttgg cgttgcggcc gcgcgcggaa ccgcgacggc
      241 gagaattcgt gttcttgctt gttttctttt gttcttgtgc cataaccttt ttgggatttc
      301 tgttgtggtg cctctgctct gtcatgcagg ggcggcgggc tggcaacccg tatgcagcgt
      361 ttcgggctta cagcagtccg gcagcgcgca tgccctcttc agccttgtca agctgagtgg
      421 cgtcgggacc gatgttcggc agacgcatgg tggtggaggg gataacgccc ttgaccttga
      481 gcgcagcctt ggccatgacg gcctgatagc cgtcgccatt caacgcgtgg acaagggggg
      541 cgagctggtt ggcgaggcga cgggcggtag tgatatcgcc gcgatcgaat gcctggacaa
      601 gctgctgcat ggggttggag gcgacgtggg caatcaccga aatgatgccg acggcaccga
      661 tggagaggaa cggcaggaac aggccgtcat cgccggaata ccaggcgagg ccggtgcgct
      721 gctgcttctc cactgcggct gcaagatcgc cggtggcgtc cttgacagcc ttgacgtgtt
      781 caagttcggc cagtcggtcg taggttccga ccttgacctt caagccggta cggccgggaa
      841 catcgtagac gatgatcggc ttttcggcgg attcgtcgac ggccttgtag tggccgacga
      901 taccgtcctg cgaggggcgg gaatagtaag gcatgacgac gagcaccgcg tcggcacccg
      961 cttcctgagt ctgttcgacc atacgcacgg tgtgtgcggt gtcgttggag ccggcgccgg
     1021 aaatcaccgg aacgtcgacg acctccttga cggccctgac cagctcgacc ttctcatcca
     1081 tatgggtgac gggcgattcg ccggtggtgc cattgaccac cagaccatcc gcgccatcgg
     1141 cgacgaggta tttggcgagc ttctgcgcgg cggcgaaatc gacgctgcca tcggatttca
     1201 tcggggtgac catagccggc agaatgcggc cgaaaggtgc tgaatcaaga aggtgcatgt
     1261 catgctcgct catagtgttt accgtacttc ccattgtgga ctcttatact acgtaccttg
     1321 attgttgctg aatgcgtggc attatggccg gagccggtgg caatgccgtg atgcgcgtcc
     1381 cggcgcgctt ccctgcctga aatgcggggg caactactgg tcgactgcgg gtcgattaca
     1441 ggtcgaggaa gtggtcgagg ccgatggtca ggccagccgg agcgtcgccg gcgagcttgc
     1501 gcacggcaag caggacgccg ggcatgaagc tggtgcggtc gaagctgtcg gcccgaatgg
     1561 tcagctgctc gccggcgttg ccgaagagca cttcctcgtg ggcgttgagg ccgcgcagac
     1621 gcacggcgtg cacgtggatg ccgtcgacca cctggccgcg tgagccgcca tcggtttcgg
     1681 tggcgtccgg cacgggggcg agtcctgcgg ccttgcgcgc ttcggcgatg ccgtgggcgg
     1741 tgtggatggc agtgccggag ggggcgtcca ccttggtggg gtggtgcagt tcgatgactt
     1801 cggcggattc gaagtatttc gcggccttgg tggcaaagta gtcggccagt acggcggaga
     1861 tcgcgaagtt aggtgcgatg aagaccttct gggtttcggg cttcggaccg ttggcgatgg
     1921 cggatttgac ctgggcgagt ttctcgtcgg tccagccggt ggtgccgacc accacgtcca
     1981 cgccttggcc gatcagggtc agcacgttgt tcaggctgac ggacggcacg gtgaattcga
     2041 tgaccacatc ggtgttgtcg gtggtgatct gggtcaggtc gtcgccggca tccaatgcga
     2101 gcgccagctg cgtatcctcg gcgttgttca ccgcttcgac cacgtgcgag cccatgcggc
     2161 cctttgctcc gaccaccgaa accttgatca tgtcttcttc cctgtctacc cgtacctgcc
     2221 cggcaatgta cccgggacgt tttccaatcg gcaatcagcc tatcaccgca ggcccctcac
     2281 gccaataccc cgtccacaga atcaagctcc ctctgacgag ccgaaccgtg aagtcgagct
     2341 ccctctgatg agccgaaccg tgaagaaaac gccggaggcg tttttaggtg agggcgaacg
     2401 acagtgagca ataattcagt tggctgaaag ccatcattaa ttgcgttcga gctggacgcg
     2461 aagcggactg agggagagat cccggcgaag ccgccaacca tccatcgggc gtagcccgat
     2521 cccgtttcac ccacgccgct tggccaaggc ccatccggcc aacgccaatg gcacggacgc
     2581 cagcgtgaag atgaatggca gcatcatgcc cacatgaggg ttggaggcgt caagcacaat
     2641 gccggccacg gacgagccga tcgacgtgcc gaccgtgcct gcggtggtta cccagctcag
     2701 tccttcggtc agggattccg ccggcaccag atccttgacg atgaggttgc cggtggcgaa
     2761 cgtcggcgag acggtcaggc cggcgaggat ttcgaagacg cccagcagga tgagattgtc
     2821 cattgacaac cggaagaaca cgtagccgac ggtcagcagc gacaggaaca tcaccatatg
     2881 cgcccaatgg gagcctttaa gttggcaaga gccgaacacc agcgcgccga tgcacgagcc
     2941 gaccgcgaac atcgccaatt gcagaccgag gaagggctca agtcccatgg atttcatggt
     3001 ggcggtaatg gacacgtcga aggacgtgaa gctcatattg aacacgatga acacggcgag
     3061 caacggcagt acgccggcat agagcaatac actcttgggc ttggcaccgc tgcgtttgag
     3121 ttggcgcagg gtcagctggt cggcatcttc gcttgcagcg gggctgccgt catcgtgcgc
     3181 ggccacgctg acctgttcca cgatgaccgg cggctgggtg gatttcagcg agaagaatat
     3241 ggtgccgccg atgccgcatg ccacggtggg cacgaacagt tgggataccg gatgcacgga
     3301 ggtggccagc caagccgcca gaatcggccc cagaataaag acgatctcgt caatggcggc
     3361 ttccatggcg taggccgtgt tgagcagctg ttcgccatct tccacaccgc gcaacgcata
     3421 ggaccaacgg gttcggacca atgcgccgaa tgagaactgc gtcagaccca tgatgatggc
     3481 caaagcgaac agcagcggaa tcggcacgcg caccaacgcc gcgaaggcga aggcgagcat
     3541 ggccacgatc tgcacggcga gcgcaagccg gcccacgcgg gcttggccga aacggtcgaa
     3601 ggcgcgggcg tagaacgggg tgacgcagct catggccagc acataggcgg cgctcatggt
     3661 gccggcgatg gtccaattgt cgtagagatg gttgagcgcc agaacgatgc cgagactcat
     3721 catggagatc ggcaatcgtg ccaccgcacc ggacaggcag aacgccttgg ctccgggcag
     3781 catgaacagg cgctgatagg gcgagtgggc agcagacgac ttcgaaaccg cgcgagtcgt
     3841 ggacacaata actccttact ggtgcgttgt ccggcacgtg cgcccgccca cacgtgccat
     3901 ccgagggtag cggttcccca gcaacgtcac gcggtgcgtt tcaaccgcgg accggtacgt
     3961 ggccagtcgt cttcatcgtc ggagatgcct tgcgatgcat aaggtctctc caccggtgaa
     4021 ggccgtccga tgctgtacag gtcatgagcc tttgggctca gtcgttgtca gattgttcgg
     4081 gaatgccgga gctcaactcg gtaaggattt cctgtttggt tttcgcacgg gcgcgaagct
     4141 cgcgcaagcc ctcgtcgggc tcactgacat agtagagagt ggcgtcgata ctgtccaatt
     4201 ccacaccttc gaccgctgcc agcagcagtc ggtacatgtc gagctgggca agtttgcggg
     4261 tgatgtcgtc tggcttggtg ggccgacgac cggttttcca gtcgaccacg gtaaagcgtg
     4321 cggtggtgct ggacgggtca agtccaccga tgaataccgc atcgagtttg ccattgacta
     4381 ggcccacgcc cggcacgtcc acgacaatgg cacgctccgt ccacgccggg atgcgcttgg
     4441 cccacgatga ttcggccaat ctgcgctgcc acgccaccag cttggactcg gtggcattgt
     4501 cgctacttat ggaagtggcg gaggcggcgg atacggcgga tgcggattgt tgagccgtgg
     4561 gctgtgggac agcgggctgc cgtgtggatg gcttgcggca ttgccgctcc cgttcggcca
     4621 aatcggccaa catggcttcc cgggaaacga atcccgcatc ctgacctgta tgcggcatac
     4681 ttgattcggc ttcatatgcc agtaccgctt catccggctt accggcattg atgaattgct
     4741 cagcccacgc atggaactgc gtgccggctt cggccgccgg agacgatatt cgtggaatcg
     4801 gacggataat gctacgccag tagaggcgct cctcccgttc gttcatgcca cctacgcggg
     4861 cttgcaggct tgtcacgttc tgacgtccgg cggccagaac gcgttgcgcc ttggctttga
     4921 ctgcctcatc aagcgactgg ccgctggcgg catcggccat caaatcggcg tcgtccacca
     4981 gcaattgcgc ccgttgcagc agtggaccgg cgtcttggtg ctgctcaagt gacgaggaat
     5041 ctgcggcgat aacatcgatg ccacgtgaca gctgttcatg cactgcgtcg ctgagggtag
     5101 ccggccacgg caattcgaca gcggcatccg tctgcgtaat cggcgtctgc catgcctccc
     5161 ccaccacggc gtcaccgtat gccgcggcat gctctccgat aaagaaccct tcgggagtgg
     5221 caatcgcttc gccttgcgcc gagatcagca tgggcttccc ggcattggcg gcttgcagcc
     5281 gttccacaca gtcctgagcc gatatcgcat gttcacgggc ttggagcgcg tcatgcactt
     5341 caagccagaa attggatggc tgagcggcgc gagagccttt gggcaccact gacgggtccc
     5401 gcccggattc gcccgtatag gcgcaatagg tcatcagggc atcttctcgc gcacgggtaa
     5461 gcgccacata catcaatcga cgttcgtcgg catgcagccg gcgcccatat tcctccgcct
     5521 gcgtcaaata ccagccggat gcatccactt cttccacgcc atcgccgaat tcgcgcatgg
     5581 agccgaacac ttcatcatca ataagctcca catcatccaa agccgccaac gatgcgatgg
     5641 ggtcggcttc cacatcggta tcatggggga atcgcggcag aatgccggca tcggcacgca
     5701 taggcacggg tacggcggtg gggttggtga gccaggagtc ggcggtctca tgatattcgg
     5761 gcggcgtcca gtgaccttgc tcggtgcctc ccgtatggtg ctcgtccagc acgatcttga
     5821 gatggtcgcc ctgattgctg gggaatccga cggcggtaag ccccactacc gcaacggcat
     5881 cccattccaa ccctttggac tggtgtacgg tcatcagttc cacatccacc ggagcgtcgg
     5941 ggagactggc ggcttcatcc tccaccgaag ccagatgatc cacccatgtg atgaacccat
     6001 gcaacgaggg agtttgctca gcggcaatct cttgcgtgta cgtatccacc aaatccacaa
     6061 tcgcatccat aggcatatgc gccaaagcgg gattggcgcg gtgctgcgga ttatgcagca
     6121 caccggccaa tacggtgtcg atgtcaagat ccaacgcttc tatgccggcg cgaatcacgt
     6181 cgcccagtgg ccgcccgacg gcatcatgca cctgacggag catcaccgcg gcccgggtta
     6241 tggcgttgcg cccgtgtgcg ctcaaccgtt caagcagttt taccaggtcg ccgcgtagca
     6301 atacgtctgg caggaatacc gcattggcca cctgatcgcg gtactcacgc accacagccg
     6361 cccattcatt cgctggtgta tccggttggg caaggccggc ctgcaccaat gcttggaagc
     6421 gttgttccgt gttctgttct tcagcaaaac gggccaacat ggtcaaatcc gnnnnnnnnn
     6481 natggtgaat cgtggcgtgg ccagcaagcg catcagcgat cccgtatcgg tgtggtcggc
     6541 ggccacgtgc agcaacgcca tcagatcgcg gatttccgga cgttccaaca gcgcggaata
     6601 gccgaccacg aatgtggtca atccggcctg ttccaatgcc gcctgatatt cgggcatatg
     6661 tgatttggac cgaaacagca cggcgaccgg cgctttctgt tcgcccggca tctgttcctg
     6721 ttgttcggct gcggaacggt gtcgtgcgat ggccgtcttg cagaaacgca ctacggcgtc
     6781 gatctcctgc ccggccgtgg cgaagcccag tactcccaat gttccttcgg gggcatcagg
     6841 catggggtcg agcgatgaga catcgacctc acgcatcaat gagctggaag gacgcgacgg
     6901 attggagcgc aacggcagcg tgagattatt ggcggcttcg agcactatac ggctgtttcg
     6961 cctggtgacg gacagcggga atggcttgta tccggcagac aggtggaaat cttgctggaa
     7021 catacggaat gcaccagggc tggcaccacg ccatgcgtaa atcgactgga acggatcacc
     7081 caccgcgttg acggcggacc tctgtgacgc atccacgtgg aacagtgctg ccagcagagc
     7141 ggcctgcgtg gtggaggtgt cctgatattc gtcgagcagc acgtggctgt acctgcggcg
     7201 cgtacgctct ccgatggagg ggaagcgctc gataagctga tacgcggcaa tggtgaagtc
     7261 ggagaattcg gccatattgc gttcccgctt ggcttgggca taggcctcca ccagctggag
     7321 cagaatatcg cgtttgcgtg tggtctccag cagcgcaccg caatgatagg tgcagcgggc
     7381 gtgcaaatgt tcggcacggt catcgagctt ggcttgccag ctggcgtcgg tatctttctt
     7441 cagtcgtttg atcttgggga ttttcggctc gtcctcgggc atgggttcat ctgcaacggc
     7501 ttgttgcaat cgattgatga atgccgaatc ccattgccgt actcgattga tcgcatcatc
     7561 gaagctggtg cagccggcac cgatcatggc actgccaatg gcatccgaca acgccagaac
     7621 actcactgaa cgccagatcc atgtgactgt cgatgacttc ggtggccagt tgaagcgctc
     7681 cggctgcgct caatggttgc gtgttctgat cgaatccgac aagcagtcca tattggcgca
     7741 cgatggtctg gaagaaggcg tcatacgtga aaatggccgg cttgaggaat gcccgatcag
     7801 acaccgtggc ggtcgtagag cccgccatat caccggccac tgcggcggag acgcgttcca
     7861 gcagttcgcc tgcggccttg cgtgtgaagg tcaggcccag gatgcgttca ggtgccacac
     7921 ctcgattgat aagcgcgatg atgcgttggg tcatcgtaaa ggttttgccg gaaccggcac
     7981 cggcaaccac caatacgtca gcatcgactg gcgcatcgat gatggcggcc tgttccgcac
     8041 tgggagtgaa cgtgctcatg cttgcctcgt ttcgaaaatc gtgtcgatct ggcctgcaca
     8101 ggcagggcag acgttcttca tacggcagaa tccgatatgg gtgtcttgcg gatgggcctc
     8161 gatgatggct gatctgctgg ccgccgcggc atagaatacg cgggcgatca tggtcagcga
     8221 ccataacgtc tgcgtaccgg cgagcgcggc gaattgcgcc cacgcttcgg cactcacacc
     8281 ttcgggagga gtagccggat ctatggcggg tatgtcgaag aaacggttgg ggtctttata
     8341 ccagtatcgg ggcgtgaacg gttcggcatt caacgagcca tcagtgaaca gcggcggctg
     8401 gaaggcacct tccggagcgt agcttctggc tggtgccgca ttctgcccga catggaatag
     8461 cacgctttgg ctgatatgcg gcattgcggc aagtgccttg gcaccgcgcg gcccgtcttc
     8521 ggggaacacg aggccaagct gatagcacac gagctgcaaa tcattgaaaa tctgtttgac
     8581 cgtgggcgtt ctgcctgttt tgtagtcaat gaggcgaatg cattcgccgc cgtctgcgag
     8641 tacgcgggtt tccatacggt cgatgcgccc ggacagtctc acggtcagat gctcgtccat
     8701 gccttccggc caccctccga ccagtgctcc catcagacgg accagcgtgt tccgaccgat
     8761 tgggtgcagg ccgggcacgg cattgtatgc gtcgagtatg tcacgcacat cgaatcgtgc
     8821 ggcaaactcc cgttcgcatt cggcaccggc caatgcgcca atgctgaagt tgctggagtt
     8881 cttgccaaga tacgtttccg gatcggcgtc gctgcgcaca aagtaatcgg cgatgttgcg
     8941 cagggtatcc gccgcggact cgtccttacg ggtggcctca tagcggttcc gggtgtcggc
     9001 tatggcattc ggatcaggac gtttgctctg atagatggca ttaagccgtg ccgagacagc
     9061 ctcgatgcga cgttccgcgc cggccgtgga gtccaagccc agtgcggcca acgcctgggc
     9121 attgtgggaa agatggtcca gcccttcctc gctgccttgc tgcgcaaccg catggatgag
     9181 ggttccgaaa ctggtggcca cggagctggg gcgaggaccg gcgaaccggt tctccagcat
     9241 ccagcacacc gggcacgccc ataggctgtc gaccgccgat ggagataagg tgaccatggg
     9301 cgcggcctgc gcatccgtct tgcctttccc tgtttggata ccgtcaggct gtacaccacc
     9361 agatgacgcg ctatccgatt gggccggcga ttccacaaac gcccagctct gcggatttgc
     9421 ggcgcgcaca ccatgctcta acaggagtgt caacgcaatc gcggcgtcct ttgcggcagc
     9481 ctgctgcggt tgagcagtgg ccagaaccgc gcgggcggcg ctgaccaacc cacgtgggtc
     9541 ggcgtccaat ccggccagat catattcggc gaatcccgca tgtgccccca ctgtggtgaa
     9601 aggcgcctgt tgccggttgc gcggatagta ttccggcaga tacccgaaga gaaagtccga
     9661 ggggctcatg ctgtcgttcc ataccgcgct gatcatgatt cgccgtgcac gggtcagcgc
     9721 gaccaggaaa ctcttcttct cgcccgacaa tacagccgcg aaccgcggat catgcccggt
     9781 tttgtcgctt tcttcaagcg ctcccctcag cacgagctcg gcaaggtctt caccaccgaa
     9841 catggtgttg cgcnnnnnnn nnnttgcgcc cggcgaggtt cggccacacg tcttgctgga
     9901 ccaccggtat ccacgcatat tcccagtggc gtccggccgc gcctgccggc gtagtcaatg
     9961 tcaccgcgtc ttccaccggt cctatgtggg ccaaggaatc ggcctgcact tccatgcccc
    10021 gaacctgtgc catgaatgac gtgatgtctc gactggcggt ggaatcatcg gcgaactgga
    10081 acaggcgcat tgccgcgtcc agccggtcat tggccgtacg cccctctgcc gtgttgaata
    10141 atgccgcacg ctgccagacc ggtgccagac cggtggcgtt ccacgcaaca gacagcgcat
    10201 agcgcggctg tacgcgctgg cttgccggca gccgctccaa gccggaagca acgctgccca
    10261 ccagatgcca caggttcgca aaagcgcggg cctgagggtc gttgccgagc accgcgtgga
    10321 tggatgcgag cgcagcctgc gccggcgcgg cggcatcatc aatggcaagc atcacgtaca
    10381 gcgcgtcaac gccaaaggcc aagtcatcgc cggacggttc accgagtatt tcgacagaca
    10441 ctggcaacga agtcactgac gacgaaaccg ctgacgtcga aaccactgac gaggatgttt
    10501 cacgacctgt ttcacgccgt gtttcatggt atgtttcacg caatgtttcc catgccgtcg
    10561 tcagagaggt cagcatggca tcatccgtgg cgagatgcgc cagcgactcc aacgacccca
    10621 tggccgattc gatcggcgca agccttgcgg gacgcccctg accgggtttg gccccggcac
    10681 tcaccaacgg gccgttcatc aacgtggcca ctctagtcct tatatatgcc gcgcaggaag
    10741 ccaacgtcat ctggcattcc gccatcccct cggcacgcaa tcgggcaagc tccacaagcg
    10801 cgaacaatcc ctgcacgaaa gtctcgtcct tcaaaggacg ggtgaccgac gaataccgca
    10861 ccggtacacc atctcggcgc agtcgttcgc cgaacagccg cacggcggca ttgtcgtggg
    10921 tgatgaccgc catgtcgttc caccgaatat gccggtcaag atgcgcccgc ttgatgcgcc
    10981 agaccacatc atccagctcc tcacgtgagg atcggtacag gccggcggtc accgtgccgt
    11041 ccgcctgata attcgctgca tcgactccgc ccagtannnn nnnnnnacgc tcggccggag
    11101 gcagcggctc gtcttccggc gagggaatca acagcgaaat acgagacgcg atcacatcag
    11161 ccatcgtcat gtgcgactga tcagcgacgg cactaccgcc aaccagctgt tcttccttgg
    11221 ccttcagccg cccttccacg gcacggcgca tcagatattc cggatacgat ccgcggaagg
    11281 tctgcaccga ttcatccggg ttgccgacca gcaccaactt gacgccggca ttccccaact
    11341 cctccaagaa gcgcatgccg gccagcgtgg tgtcttgaac atcatccaca accagcagcc
    11401 ggggcaagcc caacgcctcc acacacatag gccggtcgga atcatccgcg tgatcggcgg
    11461 cgaatgcctc acgcaccaca cgagcgcctg ccaccagcaa atacgaggca tccaaacggt
    11521 attgccccgg at
//
LOCUS       DAWBMW010000034        11530 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_1413_length_11530_cov_2.376296, whole genome shotgun sequence.
ACCESSION   DAWBMW010000034 DAWBMW010000000
VERSION     DAWBMW010000034.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 11530)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11530)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11530
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1413_length_11530_cov_2.376296"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    240..272
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6386..6395
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6672..6681
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2115 a         3357 c         3536 g         2469 t
ORIGIN      
        1 cttgaccacg acggactggc tgtcgttgta gtagctggac gagaagtcaa cggccttctt
       61 gcgctcgtcg gtgatgccga actgctggat gttgaaatcc cagtccttgg cgccgggagc
      121 gatggcggtg tcgaatgcgg tgcgggtcca caccacgtcg ctcttcttga aaccgagctt
      181 gtcggccact gcgtaggcga cggccgcctc gtagccctca ccgctttccg gcttgtcgtn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnataaccca cggctcgtag gccggctgtc
      301 cggtggcgat ggtcagcttg cccggcttgt acgtctgctg ggtgatgtcg tccgaatcgg
      361 tggaattgga tgcggtggac tggccggaac cgcaggctgc ggtgaacgac agagccagga
      421 tgctggcggt ggccgcaatg gtacggacga tgtgtcgctt attgaacatg ggtttcctct
      481 cctcaagtgt tcgtcatgca cacggttcaa accgtgcttg atgcattcgc gccaattgta
      541 tggctattgg gttacaaaac atcgatattt tcggatgctg gctatcgcca ttgcttatgg
      601 ccgactagag ccgcgcaatc tggcgcgtaa gcggacggat atctcatatt ggcgcagagc
      661 gaaatggcgt tcactgttcg agtgtgaagc cgtcgaatac gaatcccgga ctgaccacac
      721 aggagaccag cgcatcgccc tggcctggca ctgtgcgctg ccagacgcca gccggtacga
      781 ccgcatgtcc cgatacggtt acgtcggcct gatcggtggt gatgccggaa cccaaggtga
      841 ttacggttcg cttggattcg tcggattccg gagtctcgtc gctgccgccg agctccagtt
      901 tgaccgatgc cgggccatgc cacagccaga cctcatccgc atcgacctta tgccaggcgc
      961 tggcatctcc ctcgggcagc aggaagtaaa tcagtgacga tagcggtcgg cttgaggcct
     1021 catccgtgtg cggggattgc cagtcacgcg tgtaccagcc gccttcgggg tgggtttcca
     1081 gtccaaggcg ttccactatg gaccgggcgt atggactcat gtcttgcaaa gcgacgctca
     1141 taatgagatc tcctgttctt ggcgggtttg ctggtttttg gcttcttgat atcaggtcag
     1201 gtcagcggtg tcaccaggcg ttgttgtagg cttcgcgccc ggatatcacc gtggctcggt
     1261 tcgtgcctgc gccgtgcctc accagtgttt ccagcgtttc ttcgatgccg gccggagatg
     1321 atgtgtcgac ggggatgtcg aagaacgcga aatcagccaa tgcgcccgcg ttgatttgtc
     1381 caatgcggtt gcggcccacg tgcatgccca tggaggcggc gccgcccagc gtcatcatac
     1441 gaatcaggcg atgtgtcaga tcgtcgctgg catagccttg ttcgcgggcc aggtcgtaaa
     1501 gcatcgacac gtcatcaagc agatcgaggg tcggcgtgct ggagagcgaa tcggtgccca
     1561 cgcttaccag attgccttcc tcgaggtatt cgcgcaccgg tgcatctttg ccggtgttgg
     1621 taatgcgatt cgatcggggg cacaaggcca cgcccacgcc gcgctgacgt agaatacgcc
     1681 gatcctcgcc attggcccac acgccgtgtg caatgtgcac atccggtcca agcgagccaa
     1741 gctgatcgac gaactggatg gcggaggcgc ctttgcccac ttccttgagc tgccggtagc
     1801 tttcccaatc gtggtcactc cagtcggccg ccgaataagt ggtgagcacc ggcggataag
     1861 tgccggcctc gagcggggtc tccgccagat ggatatgcag tcgcatgttc agctgacgag
     1921 caatgtctgg aagatcaacg aacggttcgg actccagcgt atacggagca tgcgggctga
     1981 tgccgaggtt tggcagctgc tcctcgttca tccggcgcaa ctgccggatg agctcagtgc
     2041 ggcccttttc catccattcg gcgttgtgcc agcccattac ctcccaataa gcgatgccgt
     2101 gcatgccctg cgaagccagc gcgccggcgg cgtccaaatc ggtgacgatg tctgcggcgg
     2161 ccgtcgttcc cgattcgacc atctggcgag cgccgtcctc ggcccactgc ttccatggct
     2221 gggtcttctg cgcctcggcg tacacctcgt tgaatgccag ttcccaggcg cggaaccgat
     2281 cgtaggtgcc ttgcccgacc tgcaccatgc cgctgtactg cagatgagtg tgcgcgttga
     2341 tcaatccggg ggtgagcacc ccatcccaat gccgttcatg gatggtgccg tgagccgtca
     2401 tttcctgctt cagcgccttc agcacccagc ggcgtttacc tacatgaacc acgcggtcgc
     2461 catgtatggc aactgcgcca tcgatgatga cggggcccgt cacggggatg atgagtttgg
     2521 cggaatacag tgtcacatca tcgataagct cggtatcgac ggtcggccag gtgttgtcgg
     2581 tcatattctc ctcgctgcgt tgtgttcggt gccgtgtcta tggctatcgg tcggcgttct
     2641 ctcggtcgtt ttccgcgaat ctggtcagtc gccaattgta gccgttgtcc tccacggcat
     2701 ggctggctcg gtatccgcgc gccttgagcg cgctggcggc gatgcgctgg tcggtcgctg
     2761 cgtcaagtct gataagcgtg tgatccagtg tgcctcgatg ttccagaaga tatgcgacgg
     2821 cgctggcctg tacggcgaag ctcagatcca tgatctcgat tggattgccg cctcccaccg
     2881 tgtagttcac gccatcgccg tcggcgatta gcgtgagcgt tggtccgtcg ggaacattga
     2941 gttgcacggt gtccctgttg acctgtgggg tgaagtcgct tacctcgtcc aaggcgattt
     3001 cgttggcgat gccgccgatc actgcgagcg ccgcgccctc gtgcattgcg cgcatgtgct
     3061 caagtgtgac cgtatgtctc acgccagtgg cggacaccac catatccgcg caggggagtg
     3121 cctcgtcaat gtcttgtgcg gcgaagccgt cgaacaccgc tttgagtgaa gccactggat
     3181 cgatatcgca gatggtgact tcggcgccaa gcgcgcgaat gcgccgtgcg aagccttggc
     3241 cgaccgggcc gtagccgatg acggtgacgt tcttaccgtc gaatgcatgc tcgccgagaa
     3301 tgcgctgcat ggtggtcacg catgtctccc cagtgccgtg ggcgttgtcg aatccggttt
     3361 tcagcacact gtcgttaacg gctacgaccg ggtaggtgag ggctcccgct tcctgcatct
     3421 gctggaacgc gcgtacgccg ctggtcgttt cctcggccac tccaatgagg ttcgccgtga
     3481 gttcggggcg ttcgagcgag gcgagacggg cgaagctggc accatcgtcg atgatgatgt
     3541 ccggctgtat ttcgtccagc aggtgcaggg ccgcctcgtg cgcctgttcc gcggtccagt
     3601 ctcggctgga ttcgacggtg attccttccc ggcgcagctg tcccgacact cggggatccg
     3661 tggaatccgg gccgcaatat acgccgacga ttgcgccggc ggccttaagc ttgcgcagca
     3721 gaatcgcggt ctttggctcc agtatcagac agacggcaat acgaatgcct gagaaatcga
     3781 cgcgagtggt gaggctatcc atggtgtcac gtaataccgg catgtgctgt tccgcgtact
     3841 ccattgcctc cagtccgctt gccttgcgca tatcggtgat ggaatcgatg ctttgtgtgc
     3901 cgtcgcatag gaattcctcg ttgtgctcgt cggtcatcgt ggcatcttcg acggtcatgc
     3961 cccattgctg ggcctgagac ttgatttcgg catgcttctg tggctccgga atcgcgagtc
     4021 gggctcctgc cagtgatcga ttcgtcttct gggatcgggc tgccgcccac gctgcaaaat
     4081 cgctgtctgc tgtaatgctt ttcttcatat tccccatggt acgcatgcat gccgctgctc
     4141 cgccgctgcg cgtgtggcgt gggtgaagag cgtgcttggg cgcgttgcgt gcgtcgactt
     4201 cggcagcttc gaacaggcat gttgcatcga acaaatgttc gactgtgttg atatgctggt
     4261 cgggccagcg gtggtaagga ggtgatggcg atgggcagcg gaacggtatc gacgcaacgc
     4321 gagcatacct atattgccat tgatctcaag tccttctatg cctcggtgga gtgcgtggct
     4381 cgtgggctcg acccgttgac cacgcatctg gtggtggccg atgaaacccg caccgataag
     4441 acaatatgtt tggcggtctc accctcgctg aagaactatg gcattcctgg ccgtgcgcgg
     4501 ctgtttgagg taaaacagcg cctgaatgga gtcaacatcg agcgcgcggt ccatgctccc
     4561 ggcggccgat taatcgggga gtcctgcgat gcgtgcgacc tggaacgctc gccggggctc
     4621 aaagcctcga tggtcatcgc caagccgcgc atggcgcact atctggaaat cagcgggcaa
     4681 atatatggca tctacctcaa atacgccgca cccgagcaca tccatgtcta ctcgattgac
     4741 gaggtgttca tcgacgccac gccatatctg cgctcgctgg gtatggggcc gcatgcgatg
     4801 gcgcgaacca tcgtgcgcga aatcctcacc aaaaccggca tcaccgccac tgccggcatc
     4861 ggctccaaca tgtatctggc caaagtcgcc atggatatcg tcgccaagca catgcccgcc
     4921 gacaaggacg gcgtgcgcgt ggccgaactg aatgaaatgt catatcgtcg gctgctttgg
     4981 gatcatcgtc cgctcaccga tttttggcgg gttgggcgcg gctatgccaa gaagctcgag
     5041 catgccggac tgatgaccat gggcgatatc gcccgatgct cggtgggtcg tgcttcggat
     5101 tactacaacg aagacctgct gtatcgcatg ttcggtgtca atgccgaact gctcatcgac
     5161 catgcgtggg gctgggagcc gtgcgaaatc gcggatatcc gatcctatga gccaggtgcg
     5221 cacagcatta gcagtgggca ggtgctcacc ggccccgccg actatgccac ggcccggctg
     5281 atcaccaagg agatggctga tgcgctggcc ctcgatctgg tcgataaaag cgtgctgacg
     5341 aaccgactga cgctcgccgt cggctatgac atcggcagtc ttgattcgtc aaagctcgat
     5401 tcgtgcgctg attatgcgat gaaacgtgcc gctgagcggg cggcgaaaag ctatcaaggg
     5461 ccggtgacca tcgaccgatt tgggcgcaag gtgcccaaga gcgcggtcgg gtcgattggt
     5521 ttgggtgatt tcacctcgtc gtcggcacgt attcgtgagg ctatgacgat gttgtacgag
     5581 cgcattgtgg attcacgctt gcttgtgagg cggttgaccg tcatcgccga tgatgtggcc
     5641 actccggagg agctggccgc cggcaaacgc tatgaacagc tcgacttgtt cggtgatgac
     5701 ggggcccaat cgtatggatc cgattccacc agcgaaaaag atggagagca tgatatccag
     5761 cgtgcgctgc tggatgtgaa acgtaaattc ggccgcaacg ccgtcatcaa agccatggac
     5821 atggaagacg gtgccaccgg tcaggaccgc aaccgtcaga tcggaggcca tcgcgcatga
     5881 ccctgcaaga gccccgtgcg tacgacgaca tcatcaacct gccacaccat cagtcgcgca
     5941 agcatccgca catgtcccgc caccagcggg ccgcgcagtt catgccgttc gcggccctga
     6001 ccggctacaa ccaagtcatc gagcaaacag caaaaaacgc cgaaaccgcc atcgctcagg
     6061 ccgaagccca aggcgatacc gatttcggcg cttgacaatc gctctcgttg gaggaagtct
     6121 cagattcgtc agcctacgtg acgttcgaag cggtggaagc cggtgatctt gtcgatttcg
     6181 gccatcgcgt cagccggaat cacaaagccc gcggccttca tgttgttctc cagttggctg
     6241 ggcttggagg cgccggcgat cacacagctg atctccgggt gctgcaggat ccatgccatg
     6301 gaaagcaccg gcaggttcgt gccgagctcg tcggccacct tgcgcagctg cttctcggcc
     6361 tcttccggcc tgtcccagaa gtcggnnnnn nnnnnatgcc gagcttgcgg caggtgccca
     6421 tgatctcatg ctcgatgtaa cggtcgttta ggttgtactg cggctgaacc acggtgatcg
     6481 ggtacaggtt gtagcggtcg atgatgcgct gggcctcttc aaggcgagcc gaaccccact
     6541 cctcggaaac gccatagtac agcaccttgc cttgggcgac caggtcgctc atcgcgcgca
     6601 aggtctcggt aaggtcggtg gtctcgtcaa aacggtggca gaagtacatg tctagataat
     6661 cggtctgcat gnnnnnnnnn ncgggacagg ccgcggtcgt tcacgccttc gccggtgggg
     6721 aagtagacct tgctggaaat gaccagttcc ttacgcgggt actgcttcag cgtcttgccg
     6781 aggaagcgtt cggccgcgcc gccagaatag gcgtccgcgc agtcgaagaa gttgacgccg
     6841 gaatcgtagg cgagcttcac ggtctccttg gccacgtcct cagccgccgt gcccttgaga
     6901 tccgtgaccc agctgcccag tgcgacctcg ctgatcttca gtcccgactt gccaacgctg
     6961 cgatacttca tgtgtgctcc ttgataatca atcgtcaccg tcgtctcttc atggccggcc
     7021 gtgaacgcta acaattctta ttgaaacagt tcaaccccac ttgaacgcaa gttggcggct
     7081 attgcgacac gccggtggtg acttcgcggg cgcaacgaat gaaatcatca atgacgctcc
     7141 accgatacga tgtggccgga acgccgaact gaatggcata tgcggtatcc gggccgcaat
     7201 ccagcacgcg caaagtacgc aaccgttcgc cgtcaagcag cgctggcagg aaccgctccg
     7261 ggcataggca ggcacccatg ccgtaatggc acagttccag cagagtcagt acgttatccg
     7321 aacgggctgt gactttcggc tcaaaaccgg cacggcggaa cagcagatcg gcaatgcgtc
     7381 cgtcaatatc ttgtgggctg cctaggagaa acgggcaatg ctccagtgag gaaaggtcac
     7441 ctgcggcaat ggcgcgctcg tccggcgcgg ttctgacggc atcgacttgg ccattcgccg
     7501 cgccaccggt agcggccaaa tccatccaca gtgattgtgc gaggaccagc accatgcgct
     7561 cgttatagaa atccataagt tcgatgccgg ccggtttccc ggcgaagtcc gccaccgcca
     7621 gatcaagatc gccgttcgcc agccattgtt gaatctgatc gttgcggccc tcaatgagtt
     7681 caacgatgat attcgggcag tcacgttgca tcgccttcac aatcggcggc atgatcgccc
     7741 ggctacgcgt gtaggcaaca cccacgcgca gcacgccggc ctgatcgccg gcgatgtcgc
     7801 gcatttcttg gccaagtgca tcgcgatcgc ggcggaatcg ctgcgcatat cgcgcgaaca
     7861 ccgtgccggc ataggtcaat tcgagcggca catggcgtac gaaaagcgtg catccgagct
     7921 cccgctctag ggcggcgatg ttcgcactca gcgtctgctg cgtgatcccc aaccgtttcg
     7981 ccgccttgct gaagctatgt tccgaggcca cggcggcgaa gtattcgatg ctgagaaagt
     8041 tcatggcgat actcccgtgt gtatggcgtt tgcgtggtgt gtgtatgacg tgtcgtggcg
     8101 cgaatcaatg ccacagtatt aaacctgtaa tagatatcag tttagtctgt atttctcctg
     8161 ttatcttagg tcatggctcg tgcgaatagc gttgatgttt gcgactgcgg gtcatgagat
     8221 ggaatctgag atgaggctgt agccggaaga ggagaggatc gcatgctgga agcgggcacg
     8281 atgttgctgt tctgtgcggt gctgtttgcc agcgtcgccc tcggctggtc gattattccc
     8341 gcgctgttcg ttggtgtgct gctgttcatg gggtatgggc tggttcgccg gtgctcgttg
     8401 cgcgcgatgg cgagccgtgc ggccggcagc gtacgatccg cggggaccgt ggtggtggcc
     8461 ttcgtgctca tcggcgtact gccctcgctg tggcgggcca gcgggaccat cgccttcatc
     8521 gtggcccatt cgaccgcggt gattcacgcc gccacggtga ttccgctgtc gtttctactg
     8581 tgctcggcga tgtcggtgct ggtcgggtcg tcgttcgcca gtgcggcgac tatgggcacc
     8641 atctgcatgt cgttgggatt gtcgatgcag gcgaacccgg tgatgctcgg tggcgcgatt
     8701 cttgcgggcg tctatgtggg cgatcgttgt tcgccggttt ccaccagtgc cttgctggtc
     8761 aaacagctta cgcatacgaa cctgttcaat actattgccc gcatgctgcg cacgggtgcg
     8821 gtgccgtttg tgctgagttg tgtggtatac gttgcggctg cggtggtgga gtggctgtgc
     8881 gcgagcggtg gttccggatt tggtgcggct agtgcggctg ctacggctgg ctcggctaga
     8941 gcggctgttt caaccaacgc aagcagcata accgatatcg gcggattgtt ctcatcggcc
     9001 ttcgatttgc attggcttac cctagttcca gcattactgg tcatcgtgtt ggcgttgctg
     9061 cgtgtggatg ttcggctgct tatgctcgcc agcattgccg cctcggtcgc ggtatgcgtg
     9121 ggcgtgcagc acatggaatg ggcagaactg ttgcggctgc ttattttcgg ttaccatgcg
     9181 caaaatccgc aggtggccgc actcctggac ggcggcggca tcctctccat gctcactgtg
     9241 atggccactg tctgtatctc ctccgcctac gccggcattt ttgccgagac gcggatgctt
     9301 gctcggctgc agcaggtcat cgagatgctc ggtcatcgca tcggcgcatg tggtgccacc
     9361 atgattaccg cgacttgcgc ggcggcgctc gcctgcaacc agaccctcac catcatgctc
     9421 actcaccagt tgtgcggtgg gctgtacggc gacgacgaat ccgaacgctc cgcacaagct
     9481 atcgatttgg aagacagtgc cgtggtgatc gctccgctga ttccttggtc gatcgccggt
     9541 gccgtgccgc ttgccaccat caacgcgccc acgctcagcc tggccgccgc gaccttcctc
     9601 tacctgctgc cgctctcgcg gatgctcgcc tcggctgtac gccgccgctc gggatttcgg
     9661 actcggcatc gatcccaaaa tcaaaacccg ggactcgcca tcccacaccg gcccaaaccc
     9721 atgctccaca tgttcgctca tggtttccat cctaagtcgg gcgattgagc gcacgaggca
     9781 atcaggccga gaactgtttg ccgaattccg agaagagctg acggcgggcg aagacgagca
     9841 ggccgagcag caccagcagg gagaccagcc cgctgaccat caccagtaca tcccacggag
     9901 cccagccgag cggaatgaac gtcagaggca tcagaccagc gacgatgtcg aacgccagat
     9961 acttggcgta ctcgtcaatg gccttggccc gcagcacgat aagcagcacc acgtcaaaga
    10021 ccagcgaaac cagacacgtg atcggtatga cgaacatggt gacgacatga ttcatcgtcg
    10081 ccacaaacca gaccagcgca atcgccagca tgatatagaa atatcgaaac atgaggcgaa
    10141 gcggccgcgg cacgtgcaga atcgcactat tgatgaacag cacgctgatg atcagcgcgg
    10201 ccgccgatgc actggcaatc ggtatcggcc acgaaaacag atagctgaag aagccatgca
    10261 gcagcaaaat cgcgccggtt acaaacgtga tgatttccac cgccaaacgc ctcggacgca
    10321 gatagatctg ctgcgcgggg aatgcggcgg gggaggcctc gccgcgcaat tcgttctggc
    10381 agagcgggca acgggccata tcgccggtga aatcgacggt gcaggcatcg caatgcttca
    10441 tgccgacgct cctttctcca catgcacgcc agcgatgttc atacggccgc tgatgccctg
    10501 cgcggcgaga atccgacaca ggttcttgat gacgttcaga tcatggtaga tggtggagat
    10561 gccgatgctc aggtcgtcgc cgaacgagca gaccgtgaaa ttcaggctgg ccggtgtggt
    10621 gaggaagctc accgaacgta catggcgcgc gagtcgttcg tccaaggtga ctcggccaag
    10681 gttcgagacg gtggtcgtgg tctcgcggtc ggcgatgaac cgggccagct ccagcaccca
    10741 gtccttggca ggggagggcg caagacgcag caccggattc ttttccagcg caatcatgcg
    10801 gttcatatgc gattcgacgc gctcgcgact ggtggcatgg ccgagctgct cctgcacatg
    10861 gcgggcgatg tcggccaacg gctcatcggt cagcaatcca cccataccgt tgccgtgcgc
    10921 ggtgcgggcg gcggactgct ggccgtcgcc ggctgcgtct gggcttgttg cggaagtttt
    10981 cgcggagtcc tcatcggctt cgatttcggc cggcgtgtac gccacgtaag tcatgccgta
    11041 gaagttgcgc acggtctccg acctgaagaa ggcgcgcaag tcgaccggaa tatccacgcg
    11101 aatcgcgcga ttgcgggcgc gcgagggcat gacgtcgcga atcgcgcaca gtatcgcggc
    11161 gatgaggtag gaggtgagac tcactccgca ctgatgcgct gcaccaagca ccttgctggc
    11221 gctgacgtgg tattccatga acgtaggcgc ggcccggtcg atggggccgg ccagccggta
    11281 gatcttcgtg cctggcgcag cgccggcctt gtctttctcg tagtatttgt cgaagctgtt
    11341 ttcggctttg tcgctatccg aaccgtcgta atccgaaggc acgcccggca cgtcgtatcg
    11401 ctcggcgatg taggcgtgca gcagcgactt gaacaggttg atggcgccgc gaccgtccga
    11461 aatgatgtgc gagacctcga gattgacacg attgcggaaa tagcttacgc ggaacagcat
    11521 gcttttgggc
//
LOCUS       DAWBMW010000035        11422 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_1436_length_11422_cov_3.292337, whole genome shotgun sequence.
ACCESSION   DAWBMW010000035 DAWBMW010000000
VERSION     DAWBMW010000035.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 11422)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11422)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11422
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1436_length_11422_cov_3.292337"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6241..6250
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9852..9861
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2093 a         3419 c         3482 g         2408 t
ORIGIN      
        1 acttcgaaca cttccaacct tagacaaggt gttgcacttc tatttagaac ccgggacaag
       61 aaggagctca aggacaaggg cgtaaactgg aagtacaaga ccttcccgaa ggcccaggtc
      121 gtcagctact acaaccaggc catcaacaag gacgcgccgc acccggccgc cgcccgcctg
      181 tgggaggagt acctgtacac cgccgacgcc cagaacctgt ggttcaaggg cggcgccaac
      241 ccggtgctgc tcgattccat gaaggaagac ggcaccgtcg accaggccac tctgaaggac
      301 gccatcacca tcgaaggcga gccggtctcc tacaccaacg acgactccac ccgcatcacc
      361 gagtggctcc agaacaactg ggacaagacg atcggcaact gaggcacaga ccgatgaccg
      421 ccgcaatcgc acccattgca tcgaacggac ctgctgccgc caaggctgcg ggtccgtccg
      481 ctactcccgc ttctcctctc tcctcggcga tggccaagca ccgccaggag ctgggcacgc
      541 tcgccaccac gctcccgttc ttcgcctaca ccgcctgctt cctgctcgca cccaccgtca
      601 tcgtgattgt cggtgcattc caggatcgca gcggcaactt cacgctcgcg aacttcaata
      661 agatgttcga ggccaacacc atcgccgcat tcggcacctc gattctggtg tctttagcat
      721 cctctctgat cggcgcagtg gtgggcgcgc tcgcttccta cgcactggtc attggtgcca
      781 agccgaacgg cctgctgcgt cgtatggtct ccgccatctc ctccgtgctt gctcagttcg
      841 gcggcgtgat gctcgcgttc gcgttcatcg ccaccatcgg catcaacggt atcggcacca
      901 tgcttatcaa gacactgacc ggctacaccg tgaacccgaa ctggctcagt tcgttgccgg
      961 gcctggtgac catctactgc tacttccaga ttccgctgat gatcatcatc ttcctgcccg
     1021 cggtcgattc gatccgtcca cagtggcgcg aggcatgcga atccctcggt ggcaacacgt
     1081 tccagtactg gacgcgcgtg gcctgcccga ttctggcacc ccgcttcatc tccgcgttcc
     1141 tgctgctgtt cgcctccgcg ttctccgcat acgcgaccgc cgcggccctg ttctcccagc
     1201 gttcgattct ggtgccgctt atgatccaag gtgccatgcg caacgaactg gaccccaacc
     1261 agcagggctt tgcccaggtg ctggcattcg caatgatcat cgtcgtggcc atcgtcatgc
     1321 tgttgagcca cgccgtggaa aagagggccg gacgttggca atgaccgaaa cttccgcaac
     1381 caagccgccg cagctgcgcg ccgctcgtgc gcgtaaacag agcatcatca aattcgtgat
     1441 cctgttcgtc accctggcgt tcctgttcgt gccgctgctg gccatgctca tcttcaccct
     1501 gcgtcacccg ctgtccggcc gctggtcggc cgcgccgtgg atcgcgattt tcaccggcaa
     1561 cggcgagtcg ctgggtgccg acctgaccgt gctgtggcag ggcatcggca cctcgctggc
     1621 cctgagcgcg gtcaccgtgg tgatcatgct ggtgctgctg gtgcccacca tggtcatcgt
     1681 gcatattcgc tccaaggttt tggagcgcgc gatcgaatgg gtggccacgc tgccgctgac
     1741 catcccggcc atcgtgctgg tggtgggttt gggcccgatc taccgctggc tgtccgccga
     1801 cgtgctgagc accaacccaa tctggctgtg ctttgcctac gtgattctcg tgatgccgtt
     1861 cgccttccgc gcgatcgccg tggggcttaa ctccatcgac gtcaagacgc tggtcgaggc
     1921 atcccgctcg ctcggtgcct cctggcccaa ggtgttcttc aaggtgatca tcccgaacct
     1981 atggcagtcg attctctcgg catcctttat ctccatcgcc gtggtgctgg gcgaatacac
     2041 cgtcgcctcg ctgctcggcc gtatgaacct gcaggtcgcg ctctaccagc tcggccagtc
     2101 caattctcaa atctccaccg ccatgtcgct gctggctctg ctcttcggtg cggtgctcct
     2161 cgttgcgctc gacctgatct ccgacgcaat gcgcaagtca agggaaggta agtaacctga
     2221 tgaacagcaa ttcgattttc acccgtcgcc atgccggtgc cgatgccgcg gcttctgttt
     2281 cggcacccgc caccacgtcc gctgccgccg aggacgcctt caagaacgat cccaccgcca
     2341 ccctcgaagg cgtgcgcggc aaggacgtga ccaaggccgc caaggccctg ctcaaagaca
     2401 gcgtcaagcg cggcggcggc tccatccaga tgcgcgacgt cgtcaagatc taccccggct
     2461 ccaccgttca cgcgctcgac gacttcaacc tggacatcaa gcccggcgaa atggtcgtgc
     2521 tgctcggcgg ctccggctgc ggcaagtcca ccgcgctgcg ctcgctcgcc ggcctcgaag
     2581 acatccaggg cggccgcatc atggtcggcg gccaagacgt caccggcgtg ccggtcaaca
     2641 agcgcgagat ggccatggtg ttccaggcct actcgctttt cccgcacatg accgccctgg
     2701 agaacgtgga attcggcctc gaagtgcgcg gcatgggcaa ggcagaacgc cgcaagatcg
     2761 ccatggaaca gctggagctc gtgggacttg ccgatcaggc caagaagtac acgcagcaga
     2821 tgtccggcgg ccagcaacag cgcgtggccc tggcccgcgc gctggccgtc aagccgcgcg
     2881 tgctactgct cgacgagccg ctctccgccc tcgacgccaa ggtgcgcgtc cagctgcgcg
     2941 accagattcg ccgcatccaa ctcaccaccg gcaccaccac cgtcttcgtg acccacgatc
     3001 aggaagaggc gctggccgtg gccgaccgca tcggtgtgat gaacaagggc aagatcgagc
     3061 agatcgccgc cccgcagaac ctgtaccagc gccccgccac cgagtacgtg gccacgttca
     3121 ttggactcac caaccgcctg cccggcgcat ccaatggcga cgaggccgtg gtcttcggcc
     3181 agcgcgtgcc gctgctcgcc ggctccgcca agggcgattc cgtggccgtg ctcgtgcgcc
     3241 cggagaacct gacgctggcc ttggccggca gcagcgattc gcacgtcggc gaacgcgccc
     3301 gcgtggaggt cattcacttc cttggctcct tggtgcgcgt ggatacggtc atcacctccg
     3361 gcgaatacca gcgctggaac aagggcgaac agctcaaggc caccgtgcag ctgcccgcca
     3421 gcgagctgcc ggccggtctg gcggtgggcg atgacgtgat tgtcaccccg cgtccggtcg
     3481 ccgcgctcgc ctgctgaatg gcgttgcgat gatcgtgcgg ttctgttctg tttttgtgac
     3541 tgtacgatcg tcgaatggct tatcggcaat gatgtgtgtg ggacggcggc gatttttggg
     3601 cgattatcgc gtgccgtccc acagcaatct tgcagagtag gggactttct cggcattggg
     3661 aatgattgac gttgatcatt ccttgcccat tgatgatttc ctcccccatt cccaatgcaa
     3721 tcgaaacgcc gtgccgtatt cttgaagagt attgcacgtg tgatcggtcc gtttgcggcc
     3781 agtaactatg ggggataagt atgagcgaac caatgccgcc ggcgcagcag ccgcaggcac
     3841 ctcagcaatc gcagcagccg caacaatctc cacgcgattg gtggttcacg gcccatcgcc
     3901 ggttttcgac aatcggctcg gcgctcgcca ttatgatggt gttctggatc ggactgaata
     3961 ttctggccac tgaagcacta cgaacgctgc ttggcaccga tgtgcccgct tgggctgtgc
     4021 tgctcgcctc ctccggaccg ctgtatgtgg tggccatgcc gctgagtatg ttggccttca
     4081 ctcgcgtacc ggttattcgc acccggcagt accccatgaa ggcgggggag ttcattcaga
     4141 ttttcgtcat gtgcattccg gtaatgttct tgggcaatat gattggcagt atattgtctg
     4201 ccggagccac tgatggtcag gccaccaatc gcatcaacga cgtgattctt ggcagcgact
     4261 ggcgggtgga cgcattgttc ataggcctgt tggcaccggt ttgcgaagaa tggatttttc
     4321 gcaaggagat tatctcccgc ctgcgccggt acggagagaa gaccgcaatc atgttctccg
     4381 cattggcatt cgcgctattc catatgaacg tgttccagtt cttctacgcc tttggcctag
     4441 gactgatgtt cggatatgta tacacacgca cctcccgact gcggtattcg gtggctatgc
     4501 acatgctgat caacctgaac gggtcagtac tcgccccatt ggtgatacaa cagatcgatc
     4561 cccgcatcct cgacggcacg gtcagcgagg ccgaaatcat gcgtatggct gaaaccggca
     4621 acatgggcgg catgggcatc atgatgttgt acggcacagt gatgttgggc ctctgcattg
     4681 ccggtatcgt gctgcttatc accaagcgca agagctggga gttctatctt gcgccagagg
     4741 agttgcctgc ggggcttaaa atacgcaccg cctatgccaa tcccggcgtt atcgcgtacc
     4801 tactgttgac tgtgatgctt accggttgga tgctgttcgc atgactgtca tagccggacc
     4861 cagagaggcg agccgggaga tatcacagat tgcctgacga gctgccatct tcaacaaggt
     4921 attcggtgcc cgaaccggcg atggctcgcg ccgccatatc tttgatggtg gaaggcagca
     4981 caccctcgcg cgcgaacaca agcacttggc cggtcatgcc gtaaggggag aagccggtga
     5041 acttgaccag cggtggctgg ccctccagtg ccacatcggc ggtggctttg gttacgcggt
     5101 cgagaatatc ctgactgata gcggacgggt caccggaggc cttggccgtg aactcaagcg
     5161 tggccatgcc ttcgctgacc ggcgtgagct tggtcagggc ggcggtattg agtaccgaat
     5221 tggggatgac catcatgtct ccggaacggg tgcgcaccac ggtctgacgc caggtgatgt
     5281 cgtgcacggt gcctgtgacg ccttgaatgg tcaccagatc gccgggttgg attacgcggc
     5341 ccagcatcag gccgaagccg ccgatgatgt tggcgattgt atccttcaga cccagcgaga
     5401 tcgccacacc tccaacacca agggcggtga taagtgtggt cggattgatg ccgaatacgg
     5461 gctgcagtac catcgctgcg ccggtgaccc agatggtcac gcgcgtgagg ttcacgaaaa
     5521 tcgaggcgct gggaatctga gaattgtcca gaatcttgcg caacacgcgg ctgatgacgc
     5581 ggtcggccac aaatacgatg gccaatacaa tggtcagcca gatgagtttg ccggcgttca
     5641 cctgaaacca gcgtgcgatg tcttctgcat attcagtcat acgcctactg tatcggtcgt
     5701 ttctggtgaa gtcgtggaga cggggtggca gaaagcggaa tcggcctacg gtcgattccg
     5761 ctactcctgg cgttcgcggc aggtctggcc gaggccgcgg gccagtcgaa tcggatcgag
     5821 aatctcgtcc aactgctcgg cgggcaggtc ggtgcgttcg ccggccacct cgcgcacggt
     5881 acggccggta tgtacggctt cggcggcgat gtcggccgcg tgctcgtagc cgatgtagtg
     5941 gttgagcgcg gcggaaatcg acggtgagga ctgggcgtat gaacgcccca ggtcagcatt
     6001 gatttcgatg ccttccacgc agttgacgcg gaacgtctcc atggcgtgcg tcagcaacgc
     6061 catgccggtc agcagctcat gcaccatcac cgggtcgaag gcgttcagct gcaactgtcc
     6121 ctcggccaca gcccagttca cagtcacgtc catgccgaaa atcgtgaagc agcactggtt
     6181 gacgcattcg gggatcacag ggttcacttt ggccggcatg atcgacgatc cggcctggcg
     6241 nnnnnnnnnn gcgagccggc acgttgagat cgttgaaacc gcagcgcggc ccggaattga
     6301 gcagtcgcaa atcgtcggcg gccttcttca ggtgcacggc gaggctcttg atggcagccg
     6361 acgtggccac gtacgcgccc atatccgtca tggcggccac cggatcgggc gcggcggtga
     6421 ccggcagccc ggtgatttgc gccagatgct tgatagcgga cttgcggaat cgcagatccg
     6481 cgcaaatgcc ggtaccgatg gcggtggcac cgaggttgag ctgggccagc agcggcacca
     6541 cgcgatcgaa tgcagccaaa tcagacttca acagcgtggc aaacgcatga aattcctgac
     6601 cataggtcat tggtacggcg tcctgcagct gcgtgcggcc aatcgtcacg tcattgatat
     6661 gtttgtcggc cagatcgtgg aaggccttgg cgagtttctt ggtggactcg gcgagcggcc
     6721 cgatcgcgtc gataagcgcg agtttgcagg cggccgggta ggtgtcgttc gtggactgcg
     6781 acttgttgac gtcgtcgttc ggatggatgt atgtgtaatc gccgcgctgg cggccggcga
     6841 tttccaacgc acggttggcg atcacctcgt tcatgttcat gttcgacgag gtgccggcac
     6901 cgccctgcat cacatcgacc gggaactgat cggccagttt gccggcctca atttctaggc
     6961 aggcggcgcg aatggtctcg gccttggctg ggtcgatcag gcctaattcc tcattggcga
     7021 tggcgcaggc gcgcttgacc gtggcatagg cgcgaatcag ctcgggatgt tggctatcgg
     7081 tgatgccgga gaccgggaag ttgccgatag cgcgctgggt gtgaataccc cagtagacgt
     7141 tcgccggcac ctccatctgg ccgatgcagt catgttccag acgggtcttg ttcgatgttt
     7201 cgctcatgct tccatcttcc tgtgcgtggg ttggaaacgc cagcatgaga ggctgattgc
     7261 gccttgtgag aaacctcacg cgaggttcgt gcggcggaaa caatccagac tcgctggttt
     7321 tcggtcaagc ggtgtacccg gggaatcctg cggggccgtt cgacgatgag atggtggatg
     7381 gcgcggcatg cgtggcggcg accggcatgc cgggggcggt cgaatcctgc gcattgtgca
     7441 cgaagatgcg attccagtaa ttaagcatgt ccttttggct catggccagg tatatgccca
     7501 tggccagcac ggccacatct tctttaagca ggtctttgat ttcgatggtg tcgctcaatt
     7561 cgggccaacg ttgcagcacg gcgagcatgc cgcccacggt gaactcgacc atgagatcgg
     7621 ttttcaggtc aaggttcttg gggtcgcgtt gcaatgtgct gagcatttgc agacggcaga
     7681 agtcgcgcag cgattcggtc agttcaagcg tgctgtgcgg gcctgccaac agggccaggt
     7741 ggtcgaggcg ctgacgctgc tctggatcgg agagcgtggc ggtgacgcgg tctcgccatt
     7801 gttcctcggg attgccgttc ggatcggggg tgcggttgat cggaatcgat tcgacctgat
     7861 gcaggatggc tgaatcggcc aattcgggca ggctggagaa gtgatagtag aacgaattac
     7921 ggttcacgcc agcctcacgc accacgtcgg taacggtgat cttgcgatag tcgcggtcgg
     7981 cgagcaattc ccagaaagcg ttctcgaggc gttctttggc gggcagcacc tcggaatcat
     8041 ggcgtggacg tggcatctct tcctcctgat gcaccagcgt gatatggtca cgctgtctaa
     8101 taacttattg ggcagtataa ctattatttg tggacatgtc tatgagctaa tcggcgattt
     8161 cagttgcgtc ctccgctgat ggcgaagctg tggtgccggc atcggcatca gtatcggctc
     8221 tggccggagc tgcgctatct gctcctgctg cagtttctgc tgggatctcg gctgttgctt
     8281 tggaggtcgc agaacttggt gcggaagccg gggcgggcgt cggcttgcgt ttggccgtgg
     8341 acgccttgaa cagtatcgcc agggcttcaa ccaaatgacg ccggtctgcg ggggtcagcc
     8401 caacgtacgc gccaagcatc gccggaatcg aagcggggaa gttgctcatc acctcgctga
     8461 agctggtggc gccgttggcg tgcagcacgg tgaacagcgc atccaccgca cgctcgcgct
     8521 gttcgggcgt aagcgtggcg atccaatggt tcagctcctc gttaaacaac tgcgagctgg
     8581 aactcaaatc cggctcggtg atgaactggt caccatccaa tagccaggtg aaggcggaat
     8641 gctgcatgat gccatccgaa tcggaagaga ccacgcggca aggctccggg gtctcgaaaa
     8701 tcatgcccac aatcgacgaa ctcggcacga ttttcgtcac cttcggctgg atggtgcggt
     8761 aggcggggct ggtcacaatc tccggcggga aaccggggcc atccagtgaa tagacatgct
     8821 gaatgcgctt gcgcaccttg ggatcggcgt tcatggccgc gtaaatcgcc agattgcctc
     8881 ccttggaatg gccgaccaga ataatcggac cctcccacag tcgcgccacc atatccagat
     8941 aggcgctggc tgaacgttgc gccggcaccg gatactggaa agccatattg aaatcttcct
     9001 tccatccgat gagcgaatca tcggtgccgc ggaaggaaat aaccaacgtg cctttgtagg
     9061 tcggctttcg gcgcttgtgg ccgtcgggca gttggaaggt ggcggcggcg aattgggttt
     9121 gctcaccctg attgatgtgc tcgaccaccg cgcccaggcg aacgttggcg tagcgggggt
     9181 tttcgcctgc ggcctggaac aggtcgtggg tcaggtccgg gttgaccact gaaaccattt
     9241 gggcttcgtg cggcttgtcg tcggcatgtg tttccggcga acgatgcagc tcgcggttgg
     9301 cctcgtcaag gcttaccgat tcaagctcgg gatgccagat acccttcagc cagatcagtg
     9361 gatgcttggg ttcgaaagcg cgaatgcgtg cggcgaacga gcctgaccgc gtctcatcca
     9421 gcatcaacgt cggacagaca ctggccacat cctcatagat gagcgtggag atgatcaagg
     9481 cgtcgatttc gttgaacggc aagtcggcga aggagcgcgt ttcggtgtgc gcgtaatcga
     9541 taatgccggt catgcgcacc tcctgcttcc gttgatattg catatcccct tgggctgcgt
     9601 ggtcttgcga tcgcgtgcgg ccgtgcggct gcgcgcagca attaaccata tgcattccat
     9661 tgtagggatt gtacggtttt ggccggcgcg atcgagcaag actgcaccga cggcgaacaa
     9721 ttcgtggaag gattcccgtc ggcttgtcga aaattgtcgg gtagcagggt tgcgatgcca
     9781 gccagccgac caccctccgg cgctacgcgc catctcccgc cggcgggagg ggtaaccact
     9841 caatcctttt tnnnnnnnnn nctcgtgttc aagcaactcg gccttgatgt cgagtcggcc
     9901 gccgtagccg ccaaacgcgc ggcccgagcc gactacgcgg tggcagggca cgatgatggt
     9961 aaccggattg cgcttcactg cgcctccgac cgcctgcgcg gccatgcggc cgccgccgcg
    10021 tcgttcggcc aattcagcgg cgagttcgcc gtaacttacc gtcatgccat agggaatctc
    10081 ggccaccaga ttccacactt catgttggaa tggcgtgccg gcgggatgca acggcggcac
    10141 ctgatccgag ccggggcgct cgccggcgaa ataggcggtc agccatacgc gggtcatacg
    10201 gaacacatcg ggttcacgat cgggttcgcg attcgagtcg agaacggctt ggtggtcggt
    10261 ggcccaccac cagtcttcaa gacagagttc ggtaagtccc tcgccgtccg aggccatcat
    10321 catcctgccg atgggcgttt cgactgtggc ccgcatcagt tgtcgttgcg tcataccgcc
    10381 attatcgcgc gttgagggat gaagcccata ccgtgcctga tttcatgaag gacgggtcgt
    10441 gctgtacggt cagtattgcg tgctgggcag cgatatctga catagtgggg gttcttgggc
    10501 cggcatgagt gttctttccg acatcccaac cgtctaactc cgtttcgagg ggctgatttg
    10561 ggctgttccg gaggtctatc tccgtttcga ggggctcctc atccgggaaa ccacaagtat
    10621 aaacggctcc gggctaacgt cattacgccg tttttggccc gtgtctactc agccaccagc
    10681 cccttgaaac gggagtagac ctccggagaa ggccaaatca gccccttgaa acgcagcgac
    10741 ccctctgatt ggagtcaatc agaggggttc ggcacattgc tgatgcggct ggtccgccgt
    10801 ccgccttgag gcggaacgta accaagattc aggcgggatt actccgcaac gagcgccagg
    10861 aaggcggcgg ccacggcggc tgtcgggttc gggaattcct cgtcctggtt gaagaacggc
    10921 acgtcggcgt agttcaggaa cttcacttcg gcacggtcgc cgatcaaggc cttggcctct
    10981 tcggccagct gatcggttat cgtcatgcgt tcctgatttc cacggccatg gccgtgcaat
    11041 gtgcccatcg tttggttcta cccgctgttt atgctggcca tgcccatcgc ttggctgctg
    11101 tatacggcca ccgcgagtta tgtgcacggc ggcacccgat aatcgttgga aaaccggtgg
    11161 gacacgcggg ggcttggctt tttcctgaga ttggcgtatc tttgtcacct gttgcccgtg
    11221 aaggtaacac atgcggacat agcttagttg gtaaagcgca accttgccaa ggttgagacc
    11281 gcgggttcga gtcccgttgt ccgctctcgg atggctggtt caggatgatt gattcagcct
    11341 gaggatgacc cgggcggttg gcgcagtggt agcgcacttc cctgacacgg aaggggtcac
    11401 aagttcgaat cttgtatcgc cc
//
LOCUS       DAWBMW010000036        11401 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_1438_length_11401_cov_2.309448, whole genome shotgun sequence.
ACCESSION   DAWBMW010000036 DAWBMW010000000
VERSION     DAWBMW010000036.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 11401)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11401)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11401
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1438_length_11401_cov_2.309448"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1885..1894
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4161..4170
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2240 a         3254 c         3621 g         2266 t
ORIGIN      
        1 aaaatgcggt tggcttcggt gccgagcgta taacgtgggt tattggtcat aatgattgtt
       61 cctttcctta acgagaacat gcggtgggtg cacaatgagc attcagcggg cacgtcttag
      121 tgtttcacgc gaatgtcgaa atagtccatg accagttcgc agaacatcat attcgaccag
      181 ctgaaccatt cgcgcgtgta cttggtcgga tcgttcacgt cgatgccttc gtgcatgaga
      241 tgtgtaccgg cgtcggtggc tacaagacgg tcgaggatat gcgccttgaa gtccttgttg
      301 tcactggtca tcgcctcgat agacagggcg atgggccaca catagcgcgg cggggtgtgc
      361 ggtgagccga tgcccttgag atactcgccc tcgtagtagt acgggttttc gtcgctgagc
      421 agcgtacgtc gggttgccag atatgtcgga tcgtccggtg cgcagtatcc caagtaaggg
      481 gccgccatca aggagggcac attgctgtcg tccatgatat tgacgtggcc caggccgtcg
      541 gtctcgaatg cgtagatcgt ctctccattg cggttcgtcg ttttggcgtg ggcttcaatg
      601 ccttcggtga tggtgcggcg caactcgccg gcacgggcgg cgatatcggc atcattcaga
      661 atctcaccgc cgaagatgcg ttccagatag cccatcacca cggcggcgaa catgttcgag
      721 ggcaccagat agtggtaggt gcaggcgtcg tcgctggggc ggaagccgga ccaggtcatg
      781 ccggtgtacg ccaccggcga ccccttgccg ttattgctca gcgattcggt ggggatgtcc
      841 gtgtcgcgga tgaagaaata cggggagcgt tcatgatgct gctcggtctc gaatacgtca
      901 agaatcttct tgacgccggc aatgaattcg gcatcgaaat gaccggtgtt gccggtgttc
      961 gcatacagca tccaagccaa ctggatgggg tagcacagcg aatccacctc gtacttgcgc
     1021 tcccaaagcc aagggctgga gaagtcggac tgatcgtcct tgtcccagga ggcagcgttc
     1081 ggttcctcgt tgaaggcgtt cgcatacggg tcgatggtaa tgtagcggaa ctgctggcga
     1141 accagaccgg cgatcaagtt cgcaatgtcg tcgtctttgg cggcaagggc taggtaaggg
     1201 cgtaattgcg cggtcgaatc acgcagccac atggccggga tatcgccggt gagcagaaat
     1261 gtggtgccgt cgtcatgccg tttgaccgtg gtggtcaggg tatcggcaaa gcaggcgttg
     1321 aagttctcgg cccaatcagc gtgttcggtg ccacataggt ccgtgatgcg ctgcaagaaa
     1381 tccgagaccg acttcggtat cgcggtggtc gtcataaatt caacttcttt gtttgaggga
     1441 tatacatatc aagggaacag cattattcta aaccgcttta gttgaagaca cgcgaaagga
     1501 tgtgaagagc gtggccgtga gtacaccata tgaaaaaacc ggtgagattt cctgttcgga
     1561 gaatctcacc ggttgcggtc agtcgattcg tgaagtcaca cgaatgacga acaaaggggg
     1621 cggaatcagg cctcgttctt ctcgtccatg gccttgagct ggggcggggt gtacgtgtcg
     1681 cgcttgaatt cagtcaggaa gtacaggccg acgaccaaca gctcgatggc ggggcaggat
     1741 gaaggcggtg ccgagaagaa tcacgcagcc gataatcatg tagacggtca gcacgcgagt
     1801 ctcgcggacc ttcgagagcg ggtagctggc ggagatacgg tccacgaaga agatgaagca
     1861 gccgagcgcg aagaacgcca ggccnnnnnn nnnntcatga tgccgatttt gtagctggtc
     1921 ttcttgatga acagcgaggc ggggatggcc acgatgaagt agccggcata gaatgcggac
     1981 tgcacgaacg cggtcttcat gtcgctcagg tcgaatacgg ccttgaactg ggtgataaga
     2041 atgtcattga ggcttgcggc gatggcctag aaggcgaaca gcaccgaagt cattgaaaat
     2101 tgcaatactg gcgtgcgatc aaggtatccg tcagggcttt ccacccacgc cacgttcttg
     2161 gatgcggtct tgtccatgat atgtattccc tttttctcta taaatggacg tatgacagat
     2221 cgatcgatca atggatgatg actagtgaat agtgatagtc ctgttgccgg gttccatcag
     2281 ggtgttacat gatttggcag aattcgatga cctcgtggtt cgggcccagt acgttgaagt
     2341 aacggatgcc gttgctccag aaggtgtcga tgtgctggat ggagccttcc gcgaagttca
     2401 ggccgagctt ctgcgcttca gcgaaggacg cttcgatatc cgtcgaatcc agcgcgaagt
     2461 ggttgatggc accgttttcc atcggggtgg gatccatggt ccacacctcg agggtcaggt
     2521 tgttcaggcg caggaaggtg cagcggtcct caccgttggg atagatgccg aggcattcga
     2581 agccgagacg ctcgtagtag gcgatggtgc cttcgagatc cgtggtcgga atgccgacat
     2641 gctggacgcc ggtgacggtg tcacgaatgg acatggtaac tcctttttaa aacaggatgg
     2701 ctttgccggt ataggacggc aaagccatcg ggaaaatagg tggttttgga ttacttgccg
     2761 atttcagcca ggtagtcggg catctcggcc gggtactgcg gccaggcacc ggcctgggtg
     2821 cagacgtagg cggcggtgtt gacggccttg cggtgggctt cggcgagcgt gtcgcccagc
     2881 aggatgcggg cggtgaaggt gccggagaag gagtcacctg cacccacggt gtcgttcacc
     2941 tcgacgtgcg gagtgtccag cgtggagacc tcgcccttgc gggagatgat ggtggagtag
     3001 gcggagccgg cggtcagaat cacgtagtca aggccgaatt cctcgatgaa ccagcgggag
     3061 gcatcttcgc cggaggtgcc gcggatgtcg aacatgtcgc gcaacagcag gagttcctcg
     3121 tcgttgatct tgaagacggt ggccgccttg aggagttcct cgataagttc cttggagtag
     3181 tggtcgccgc gcaggttgat gtcgaagaac ttcatcgcgc ccggcttggt gtgcttgagc
     3241 agctccgtga tggtggcgtg cgattcgggg gagcgcaggg ccagggtgcc gaagcagacg
     3301 gcgtcggcct tggagaccac gtcgatgagc tggcgggtgt acaggatgtg gtcccaagcc
     3361 acgcccttga cgatggtgta ctccgggatg ccgttcttca gtgcgacctc gacggtggag
     3421 gtcggccagg cattgcgctg gatgacggtg ttgataccgg cctcgttggc cttggcaatc
     3481 agttcgtcgc cgagttcatc ctcgccgacc gcgctgatgg cccagccttc ggtgccgttc
     3541 ttcatcgcgt ggtaggcgaa gttgacgggg gcgccgccgg ctcgcttgcc gctcggcagc
     3601 atgtcccaca gcagttcacc gaggctcaga acgatcgggg tagtcatggt tggtttcctt
     3661 tcctttgttt cctttatcgg gtgtttcgta tggaaaatct tgtgatgtcg gcgcttggtt
     3721 taggagacgt cgaagaacac cgaggggtgt tcgaggaatt gggtcactgc cactgctgcg
     3781 gcaccggaga ccgccgcgtc ggcggacaag gtggataggc tgatggaagt ggaggcattg
     3841 atttcgggga tggcccgttc ggccaccgtg gcgcgcacct catcgagcag tatcgggccg
     3901 gcttcggaca cgatgtcgcc gatgatgatg tgctcgggat tgtatccatt gaagatggtg
     3961 acgcatccgt agccgacgta tcgggcgact tcgcggacaa tggcgattgc cgcttcgtcg
     4021 ccgtcgccgg ctttggcgaa cagggcgcgg gcggcttcgg tgtgggtcat atcggccgcg
     4081 ccgggaacca cggtgccatc ggcgatgagc gtatcgtgaa tggcgggggt ggagcagtag
     4141 cgttcaaggc agccgatgtt nnnnnnnnnn tgttgccgca atcgcagggg cggccgttga
     4201 cgtcaatgga gatgtgtccg atttcggtgg cggcaccgag gaagccgttg atgaggcgac
     4261 cgttgtcgat gacgccgagg cccacgcctt cgccgaccag ataataggcg aggttgccgt
     4321 cggcgtgcac cgacgggtcg aagagataat gtgcgagggc tccggcgcgg gcgtcctgtt
     4381 cgatgaacac aggcacgcgg aaagcggtga cgaattcgtc gatgaagttg attctgcgcc
     4441 agccctgcat gctggagacc acggcggtgc ggcccacgtt gcgcaggtag ggtccgggca
     4501 cggccatgcc gatggccacg atggatgggt cgttgtcgag cagttgttcg acgcgctggt
     4561 ggatggtggc aatggtgtcg tcgatggtgt tgtcgtagac cgtgggcagg ttctcgatgc
     4621 tcagggcgtt gccgcacagg ccgaatacgc cgatctgcac cagcgagcgg gcgaatttga
     4681 tgccgatgac gcggaagcgc gtggtgttga gcttgagtcc gatggaacgg cggttcttgg
     4741 agccttccag atcgccggtc tcttcaatca tgccggcctc gataaggcgt gcggtgattt
     4801 tggtgatggc cgcgggggtg agttccagtg ccttggcgat ttgcgcgcgg gaactgatgc
     4861 cgttgtgata caggtgcttg acgatgcggg agcggttgga ttcggagaca ctggcctggg
     4921 aagtggcgtg ggtggtgttt gttgccatcg gtcttaagtc ctccttgtct gcaccgctgc
     4981 gagttgttcc gctcgtcttt gaatctgaac ttgattaaga tattaacgct gttaattgat
     5041 tgacgcaatc aatatgtcat tcggcgtgtc gtgcatgtga gtgtcgtctt gggtgaattt
     5101 gttaagatag ttgtcaatag tcagatgtac actgatatca agcgaaacgt tcgatgaaga
     5161 acaggggagg actgatgtcc gaaaacacta tgaacaatct caaaatcggt gttgacgtgg
     5221 gcggaacaaa aatcgaagct gtgctggttg acgcaatggg cacggtattg ggttccgccc
     5281 gtattcccgc cagacatggc aacgatgccg tgatcgaaga catcgtcgct gtagcacatc
     5341 aggcagcggg tgagcgtttt gacgaggtga gggccatcgg tgtcggtacc ccgggcacgg
     5401 tggattcggc cagcgggcat gtgggcaata tcgtgaatct cgacgtggtc tcgctggata
     5461 tggggccgtt gatctcgcag cgttcgggcg tgccggccca tgtggaaaac gacgtcaacg
     5521 ccgccgccgt cggcgcggcc accgtgttgg gcggtgcgga cggcatggcc ggtaccattg
     5581 cgttcctgaa cttcggcacc ggactggctg cgggcattgt cgaaaacggc gtgctgatgc
     5641 atggctatag cggtgcggcc ggcgaaatcg gccatatccc agtcgaacct catcgcctca
     5701 agtgcccttg cgggcaatac ggatgcttgg aaaccgtctg ctcgggcgca tccgtgggac
     5761 gtctgtggcc caacgccgac ccgccgatgc ccgacctgat tcgtcgcgcg aaaaagcggg
     5821 aggccgaggc cgtcgatgtg ctggacatgg tagtgcgcgc catcggcgac actatccaga
     5881 ttttggccca atccgttgac ccgcgactga ttgtcttggg tggcggcatg gccaaaaccg
     5941 gtgaaccgtt ggtcgaggtg atcacggctg agctgcgccg gcgcgaatcg caatgccgat
     6001 tccttgaaac gctggacctg ccggcccggc tgcgtctggc acccgtcgat caaccggtcg
     6061 gagccatcgg tgccgccatg gcggcatagc actggcatag tgccggggca tggaagatga
     6121 ggcgtcggat ttgtctccga gaccgcatct tccatgcggt cctatatcgc tttatgtcgt
     6181 gctgtgccgc gcttggcctt gcgcgtttcg ttgccggggt ggccagcttt atttggccaa
     6241 gtgttggaag accacggcga tggcctcgcc ctgtacggtg atgtcgggtc cgcattcgca
     6301 gcgccggatc accgtgtgtt tatggaagga cgaggacgtg gcggcatcca gatactgctg
     6361 tgccgcgtca atgaacgtct cgttgatgat gttcggctgc ccatagacgc atacgtctgc
     6421 catatccaaa aggcccacag gcatggacag ggcctggccg agatagcggc ccgcccgagc
     6481 aaggataccc tgatgctgct ccacgtctgc tgcgctgagt tgcttcttca atgcggttcc
     6541 ggaaatcatc gtctccaagc aaccgtgctt gccgcaaggg cacagcggcc cgtcaagatc
     6601 gatggaaata tggccgagct cgccagccgc gtgcagttcg ccgattaccg gcgtgtccga
     6661 gagcagaatc gccgaaccga tgcctctttc gatgcgcacg aaaagcatat tcggcccgcc
     6721 ttggccaaag aaacgttcgg tgagcatggc tgcggtggcg tcgttgctga cgtttacttc
     6781 aagtccgaaa tgttcggcga ttggcgtgga caggtcgaga ttgttccagc cgcgcatcgt
     6841 ggaggagcgg accacgccgt tgtctaccac gccgggggag gcgactccga tgccgatgac
     6901 ctcgccatcg gacatgccca gcatcttctc aatgagctcg atgatcacat ccacggaaac
     6961 gaatgagccg gtgttcaggg tgacttcggc gtgacgcagc ggctggccga gcagattggt
     7021 gactgcgccg tgcagcaggt gctcctgcgt caggtcgatg ctgatgatgc gcagtcgatc
     7081 gatatcgatg ctcagcagtg tgcctcgttt gcctttgccg ccgctgggtg cctgaccggt
     7141 ctcgcgcagc aatccttcct ccagcatccg accgaccaca tcggaaacgg ccacgcggct
     7201 caatccggtg cgtcggccga gctcggcgcg cgaatactgg ttggtgggga acaacaacct
     7261 gaagataagt tggcggttct tgcggcgcac atcggcgggg gaagccttgg ccgcatggtc
     7321 gtcggtcgtt gtgttgagac cgagatatga catgtttcct cctgcctgat ggtgagcgag
     7381 tggacggcga ttgttgttcc atacacagct tagacggtga agcgcaattt cgtcgttgaa
     7441 actgttatga aacttcacta ataaattcca gtgtacctgt gtgaagcgtc aggaaacacc
     7501 gtgagtgttc ataaggtcca aaattgttag tatcattaac aaatagcgaa tcaccaatcg
     7561 gcgctacgcc gaacggtggt tggaacgccg cgcggcaccc ggacgtgaca aggatgttgc
     7621 cattatccgc aagacgggcg gcggtacagc gactcgcatc gggcgggccg cacggaactg
     7681 gaggaaaccg atgccggaaa tcatcattgt caagaacgaa gccgaggccg gcgagatcta
     7741 cggccgttgc gtggcggacc tgatcaaagc aaagccggac gccgtgctgg gcctggccac
     7801 tggctccagc ccgctggccg cctatcaggc gctcgccaag atcgtcaagg acgaggccat
     7861 tgacgtttcg ggcgtgcgtg gtttcgcgct tgacgaatac atcgggctgc cgctgaccca
     7921 tcccgagtcc tatcatgcga ccatccatcg caccgtggtc gaaccgcttg gcctggaccc
     7981 ggccaaggtc catgtgcccg gcgacgtact caacggcact ccgctcgaag acggcgacaa
     8041 agtcgcgctg gccggcccgg cctatgatcg tgccatcgaa gccgctggcg gcatcgacgt
     8101 gcagatcctc ggcatcggca ccgacggcca cgtcggcttc aacgagcccg gttcctcgtt
     8161 ggcctccggc actcgcgtca agaccttggc cgaacagacc cgcatcgaca acgcgcgctt
     8221 cttcgacaac gacatcaacc aagtgcccac ccactgcatc acgcagggta ttggcacgat
     8281 catgaaggcc cgtcacctcg tgctgctggc gttcggtgcc ggcaaggccg aagccatcga
     8341 ggagacggtg gaaggcggcg tgtcagcgtt ctgcccggct tccgcgctgc agatgcaccc
     8401 gcacgccacg attatcgtgg acgaggaggc cgcgtcccgc ctgcgccaca aggactacta
     8461 ccgttatgcc tacacccaca agccggcctg gcagggcatc tgagttatcg tgtgctggct
     8521 cccgccagcg ggagcgcatg agcggtaagg agaacaacat gtctgatagg aacgagatag
     8581 tctcgcgcgt gaccgcggca ctggagggca agccggctcc ggtggcgatt cgtaacgcgc
     8641 gcaaggtgga tgcgcgcggc gaacggcgcg gatattgggt cgtctccgat gccgccggcg
     8701 tcatcgtggc ggccggcacg ggggaggaga ccttcgagca ctactgccgc acggtggggc
     8761 tcgacccggc cgatcgcaac gcggtcatcg acgccgaagg ccgtattctg acccccggct
     8821 acgtggacat ccacgcgcac ggcgcatggg agaagagctt cgatgacggg ccggacggca
     8881 tcgacgtggc gcgggccggc cacgccgtgc atggcaccac gcgccaggtg ctctcgctga
     8941 tcaccaaccc cgtggacgtg atctgccgca acatccgcac cgtccgcgcc acaatggagt
     9001 ccggccgccc cgacatccta ggctgccatc tggaaggccc gttcctggcc ctggcccgca
     9061 agggcgcaca tgacccggaa tgtctcaagg atccggtgcc tgacatcgtc gataagatgc
     9121 ttgaggcctc cggtgccgac cccgcctccg gcaagcttgg ctgcatccgc cagatcacca
     9181 tcgcccccga actgccccac ggcatctccg ccatccgcca gttcgccgct gccggcgtgg
     9241 tgccggccgt gggccactgc gacgccgact acgagaccgc caaagccggc ttcgacgcgg
     9301 gcgcgggcat catgacccac atgttcaacg cgatgaacgg cctgcatcac cgcgaacccg
     9361 gtccgattcc ggccgcggtg gaggacccgc gcgtcaccat cgagctcatc aacgacggat
     9421 tccacgtgca ggatccgatg gtgagcctct ccttccggtt cgcgcctcac cgcacggcct
     9481 tcgtgaccga cgcgatggcc gccaccgact gcccggacgg cgcctacaag ttgggcgcgc
     9541 tggacgtgaa cgtggtcggc gggcatgcgc gcctggtctc caacggcgcc atcgccggct
     9601 ccactctgct gctggaggtc gccgtgcgcc gtgcggtgtg cgaactgggc ttcagcccgg
     9661 tggacgcggt cgaggcggca actctgacgc cggccaaggc gttcggcttc gaccggccga
     9721 acccggtcac cggggcgccg atcggcctca tcgcccccgg cttcgccgcc gatttcaacc
     9781 ttgcggaccc ggccgactgg acggtcgaac aggtgtggtg cgcgggccgc aagctcaagt
     9841 agcagtaggc cgaccggttg acgaatggaa ggcattcgtc tacgaacatg gtcacatggt
     9901 gagatgatag tatatttgtc tcaccatgtg accgttattc acgtcgtaat acaagaatcc
     9961 gtaatactgc tcgataccgc ataaaaccgg ccttggtcgc cacaagcgaa acataagtcg
    10021 atgactatgg aaacgcagag gtaacacatc cgcaccatag tcaggcggta atccacgccc
    10081 gcagtgatga cgtggaaaca agttatgaga gaggattcct gttgaattcc aagaagattc
    10141 tggccgccgc cgtctccgtg gccgccatcg catccctgac cgcctgcggc ggtgtgaagg
    10201 atgacaccac cgcaggtgcc gattccggca acgccatcac catcggcacc accgataaga
    10261 tcaccagcct ggaccccgcc ggctcctacg acaacggttc atacgccgtg cagatccagg
    10321 tgttcccgtt cctgtacgcc caggactaca acacctccga actgagcccg gacatcgccg
    10381 ccgatgacgg tacctggaac gatgacggca ccgaattcac cgtcaagctc aaggacggcc
    10441 tgaagtttgc caacggcaac gacctgaccg cttccgacgt caagttctcc tacgatcgca
    10501 tcaagaagat caacgacccg aacggcccga gctccctgct cgccaacgtc gaatccgtga
    10561 ccgccaagga cgacaccacc gtggtgttca aggattccgt gccttttgac gtgactctga
    10621 agcaggtcat gtccagcccc gccggcccga tcgtcgatga ggataccttc gacgccgaca
    10681 agctcgccga cgccgacacc atcgtctcca agaaggcctt cgccggcccg tacacgctga
    10741 cagccttcaa gatcaacgag tccgccgcct acgccaagaa cgactcctac aagggtctga
    10801 ccccggccaa gaactccgtc gtgcaggtca agtacttcgc cgacgcctcc aacctgaaga
    10861 tggccgtcca gcaaggccag gtcgacgtgg cctaccgctc cctgagcccg accgacatcg
    10921 aggacctctc caaggactcc aaggtcaagg tcgtcaaggg cccgggcggt gaggagcgct
    10981 tcatcgcctt caacttcaag attcagccct acggcgaatc ccagcccgat gccgacgcca
    11041 acaaggccaa ggccgtgcgc caggccgtcg ccaacctcat cgaccgcgaa gagctctcca
    11101 ccaaggtcta caagggcacc tacaccccga tgtactcctt catcccggac ggcctggccg
    11161 gtcacgatga cacgctgaag tccgcctacg gcgacggcaa cggcaagccg agcaccgaga
    11221 aggccaagaa ggtcctcgcc gacgccggcg tcaccacccc ggtcgacctc aagctccagt
    11281 acaacaacga ccactacggc tccgtctccg ccgacgaata cgctgccctg aagtcccagc
    11341 ttgaggcagg cggcctgttc aaggtcgaca tgcagtccac cgaatggacc cagtacaaca
    11401 a
//
LOCUS       DAWBMW010000037        11392 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_1444_length_11392_cov_2.387404, whole genome shotgun sequence.
ACCESSION   DAWBMW010000037 DAWBMW010000000
VERSION     DAWBMW010000037.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 11392)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11392)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11392
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1444_length_11392_cov_2.387404"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1633..1642
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2312..2321
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4377..4386
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5076..5085
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5703..5712
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11060..11069
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2311 a         3346 c         3260 g         2415 t
ORIGIN      
        1 cggcaagggc gatacagcaa gccgtatccc ttagggctga gcgaggtatg cacaccggcc
       61 atgaagtgca acatgaaggt ttcgtccgtg agtccctgtg cggagcgtgc gggggcaaag
      121 cctatggtca tggttttcga cgagcccaac gcttgcgcgg catagaccgg cgtccaccca
      181 agtttggccg cagcctcctt gactcgtcgt ttggtttccg cggaaacacc aggccgatcg
      241 ttgatggcaa aggacaccgc ggtttgcgaa atgcccaact catccgcgat gtcttttaag
      301 ctcacacgct gcgtgcctgt ggacgatggt gatgcctcgc gagcaaatcc catgaataat
      361 cctccttgca gccacgctgc gaataccacg ctacaacaga ttatttccca ctttgggccg
      421 catgtaatca gctgtgttct aatacctatg atatcaacaa ctaaaacgtt ttagtctatg
      481 ggtctcagca tatgttcgga tataaaaagc gggatatagg gggtgtctac agggacaacc
      541 accccatatc ccgcttaagg cacgggtatc aacgcacccg catacgcagc gaaactattc
      601 catcttgcta cgagcacgaa gcaccaccgt gcccgctccg agcaacacca ctgcggcagt
      661 cacaatcgca ataatcgaag agcccgtctt actcagcgaa gagtccgatg cgccattctt
      721 atcattgttc ttgttgttgt tctggccagg cttgttgtcg ccgggcttat tcggcttgtc
      781 cggctggccg ggcttgttcg gggtcgccgc gaccttggtg aattcgatgg cgacaccctc
      841 accgatggac ttgtcgccca gtgcggcctt caccgtgtac ttgcccacct tcgtggacac
      901 gacgttcagt gaagccacgc cagcggcatc agtcttgaca gtggcgttgc cgtcaacggc
      961 cgtgacaccc tcgggcagcg taaacgccac ctcggcgttg gtcagcggat tgccgaacga
     1021 atccttgacc gttgcctgaa tcgtacgtgt agccttgccg tcggccacgg ccgagccgtc
     1081 aacggcggtc agcgtggact ccttggcatc cggctgagtc gggtcagaga ccgtgcactt
     1141 ggccagttcg tcggcggtct gaatcttcac cggttcggtc ttgttgccgt tgtaatcctc
     1201 gacctgcacc cacagttcat ggccagaggg gatggacttg gtgtaagagc tcggcgtagt
     1261 gcgaccacgg atgatgccgg tgattggcgg agtgctctgc gagtaggtgt aattggcacc
     1321 cagctgcttc tcctgctttt caccgttctc gtcagtccac ttctcggtca tgtagaggta
     1381 gcgccagtct tgagtctccg gacggaccag agtgactcta tcgccattcc atgtcaccga
     1441 gcaagccgga tacggctcaa tggtggcatc ggcaaataca cctgttgccg tggcggtagt
     1501 gccgcctgcg ttatcgacgg tcaccacata gcgggaaccg tcaaccggcg cattcggaat
     1561 cgtcgcttcg ctcttatcgg cagagacctt gacggtctgg gagtacggct gcgcggcgta
     1621 acgccacgaa cannnnnnnn nntcagactt gacggtcacg gtcttctcgc cggatacctg
     1681 cggattggtc caggagacct tgacgttacc atccttgccg ggttcgactt tcacgccaga
     1741 cacggccgct gcttcgttca gcggcgcttc ggcggccagc gagcgcgaac cgtccgcacc
     1801 cacggcgtac agcttcagcg tgccggtctt ggcaggcagg tgcttgacgt acagggtgtc
     1861 gtcgtaacgg ccaccgagga aggtgttgtc caagtagagc aggtagttct tggcagtaga
     1921 gtaatcggcg agatcccact tgacggtcag ttcgtcggag tccgcgtagg cgcgttccac
     1981 ttggaagttc gtcggagcag ccggcgtgta ggcagcaccg tcggtgacgg taatcttgcc
     2041 gatgttcacc tggtaattgt cgattgggtt cgccccggcc ttgacgatca ggcccagggt
     2101 ggcgatcttc ttacccgcgt actgagagag gtctacagtt gcggtcttcc agccgttgct
     2161 tgccccaccg tctgccatgt ccaccggcac tatagtcttg gtgtcgtcgg cgaaaatgag
     2221 gccgagttgc agcttggaat cgtcgctgtt gagcttgttg taggtcagtt cgaccttgga
     2281 gccggcagcg acgctcaagt cggtcttgta gnnnnnnnnn ngtgttgtcg ctggacagct
     2341 tgccgctcaa cacctgcgaa tcgccgcctt cgtagccgcc gaccttggtg tagttaaagc
     2401 gtggtgccgc ttcatatttc ttgccatagt cgaagtcggc ttgtagcggg tcggaatcgg
     2461 catcaatcca ccactgccat gtcggcagaa tgtcttggag gttgatattg ccccactgct
     2521 ggttgctggt ctgttcgccg ttgtctcgcc attcgagacc atgaccggtg ttgaacgatg
     2581 tggagaagac cggaccgccg atgacggagc gctcggcgat ctgcgaagcg aaacccttcc
     2641 agctatcgct gctcgtacca tcatcaatat acggatcaga tatatccgtc gtgcccttct
     2701 caccggtgga cgaaccagtc cacagacggc gttcgcggtc gaatacctgg ttctgatact
     2761 tcgggtagac cttcttgtcg tcacccaact cgtgggagac gaaatcggtg ccaagtgccg
     2821 caaggctcac atagggcttg ccgtcagcgt caaggttgac attcatataa ttcgcgttgc
     2881 tggcgatgct accaaatttc ttctgtccgg cctcgatacc cgcgaatacc gcgtacttcg
     2941 gatcaatgcc aaggctcttg gcgtgatcag ctgaatcttg tagcatgttg ccgctgaacc
     3001 agtagttcag gaagatcgaa tcagagattt tacccttgtt cggatcctgc acccacggcg
     3061 aattggcatc gttgaacatg ttttgatagc tgacgccgcc attcgggtat gtggcggagt
     3121 cgtaccactg gatataaatg ccctgatcga tgatctgctt catgaagtct tggtaggccg
     3181 gcacatcagc agaattcaca ctggattcct ggttaacgaa gtaaccatca aaaccatagt
     3241 acttggcaat ctccaccagc ttgtcagcca ccggataggt gccgtcggca cgcttgcctt
     3301 gaagcaggtc cttataggtc tgttctcctc ggtcgttgcc agaatagaag atcgtcgcaa
     3361 tcgacttcgc gccattcttg tgcgcgacat tggtatatgc ggcattcggc agattcaacg
     3421 taccgaactc aaaccatttc tgagtccagt cagactgggc cttctcgtcg tagaggctct
     3481 ttggcacgtt cgcagtcggc tgaccatgcc aggtaccgta ataatccgtg tactgccagt
     3541 agttgaacag gtactgcgag aacacgttgt tgtcgtggaa cgactcgaag aacgcgttgc
     3601 cgtagtcgcc agcaaggttg aacatctccg tatcggccgg caggttcggg tctttctgcg
     3661 tcgcggcgtt ggcggcgatg cgggtctgca acggtacacg ggagcgcagg tattccgagt
     3721 ccttatccgc gtccgggttc cagttcagaa tatccagacc ggtatagcca tgctgatatg
     3781 gctggttggc tcccgcagac tgctccggat ggctggtatc ggcatactgg cctccccacg
     3841 gcatggtgtc ggtggcgctt gccggtgcgg ccaatgccgc cagcgtggcc atcgacagca
     3901 ccgctgccaa gccagaccgc agccatttcc tgtgtcgagt ccgggcagaa gcacgcccag
     3961 cgtgatcacc tgatgacaat ctcatccata atctcctttg atttcctccg actcacgccg
     4021 gaaatcaacc gtctatgcgg cttgagtaga cgggggctca aaccggtagg acggtttgag
     4081 cccacgggat acggcgatat ttcaccaacg ccatatccca cattctcttt ttctctcacc
     4141 caccgaccat atcggccgca atgagtaggt catgagactt acggaacata cccctctccg
     4201 acttgtctca cgcacatcgt cagcgatgta tcacgatgct tacgctggta tcgcagtcgt
     4261 ctgtatgtcg ggtatcatcg ttattcaaag cggctgtcag ggcaaacgtc acacgcacat
     4321 tgtcatcagc ttcgtccatc agccgttcaa agggtaccga gaacgatacc gcgtggnnnn
     4381 nnnnnntttc gcccgcctca agcgtcaggg caacggaatc gaccagcact gccaagcgtg
     4441 ggatgcgacg accgccactg acgtgtgcga agaggttcac cgaaccggcc gtcttatggt
     4501 cgccggcatg tgccgtcacc tgcaccaaca cctcattcgc gcgggattcg gcatcggcat
     4561 gcatcccact aaacgtcact ccagccaaag aaccggctcc gtagccaaac tcgcgcaata
     4621 ccggttccgc cgcatccttg tatacgtggg cggcactttg ccggtcgttg tatcgaaccg
     4681 gaatggctgc gggatgcgca ggcagcgtta cgggaaggcg gcccggcaaa ccggcaccgt
     4741 caatcagcac atcagccacg gcctgcggac catactggcc agcatatccg acccaaatcg
     4801 tcacggcgga ttgacctatt acatgagtca gcacatgcgc cctgccgcac accaccaccg
     4861 aaacaaccgg agcggtggtc aatgccacga cttcgtccag catttcatcc tggtgccaag
     4921 gcagacggat gtcggacaaa tccacgcctt caccgcaggt ggcaccgtat tcggctacgg
     4981 cctttgccgc accattgtcg tcgaattcac tgtcatagct gcgttcgctg gtgctgccgc
     5041 atacgaatac cacggctgcc gcactcgccc accgannnnn nnnnnctcgg cttggccgcc
     5101 aagcaaaccc ggtctttccc aaggcgtgcc accaattgcc ggtagatgct tgactgctca
     5161 tcggcgggca gaggggcggt gtaatcgccc aggaagcagc cgaaatcgtc ggcgaatggg
     5221 ccggcgacaa tcacagggcc ggcttgggca tcggtcagca caccccgaat gctgtcgagc
     5281 gtaacggcag agcggccatc attaatcagc gttatcgcct cgcgggccat gcgcttggcc
     5341 tgctcacgcc catccgcagt ggcctgcgcg atggcatcgg catctggcat ggcaattgcg
     5401 gcggtatcgg caccatcttc gggcaagaga ccgaacatgg ctttgagctc cagcacacga
     5461 cgcaatgccg tatcgacggc ggccgccacc tgttcgtcat ccacatattc ttcgaggcgc
     5521 gcgaatcctt catcccacag ggagacatcc acaccggcaa gcagtgcggc gcgtccggca
     5581 gcgggaatgg agccggccat atcttcaagc cggtccacgg ccagaccatc ggccatcacg
     5641 atgccatcaa aaccgagctg gtcacgcaga taatcctcga gcaaccacgg gttggcacaa
     5701 cannnnnnnn nngcacggaa tcgatatcgt tgtaggcagc cataaagccc aaggcaccgg
     5761 cacgcacgcc agcggccacc ggcggcagat gaatctcgtg aagatcgtgc gggcccaata
     5821 cggcggactg cccgttgcgg ccacccacag cctcgccttg cgccgccaaa tgcttgagca
     5881 ccacggccac tcccccgcgg ccgaccttgc tgcgatgctc accctgcata ccggtaacga
     5941 tggcctcgca catgcgggag gccatcagcg ggtcctcgcc gaagcattcc tcgcaacggc
     6001 cccaacgcgg gtcgcgggcg atgtccaaac cggataccaa tgcaatatga ataccgctgg
     6061 ctgccagttc cgcggcgact gcggcttccg cttcctgcac tccttgcgaa tcgaatgtgg
     6121 cacccaatcc cagattggtg ggcagcaccg cgccgcccag cgcctgatgc ccgtgaggag
     6181 cctcttcgct gagcaatacg ccgatgccat gtgcaccgcg ctccaacacg gtttgctgca
     6241 ccacggcaac cgcctcaggc cgttcctcag ggcgtatgcc attgccccaa tgctggcccg
     6301 accacgggtc agctcgcagc aggccgtaca aagcgccaag cccgccccac cggtcgatct
     6361 cttgcttgag ctcgtctgag gtcacaagcc gtcctgcggc attgcgttct acggacttcc
     6421 aaccgaacag acgctggttc agctgaccaa ccttctgcgt gaagctcatc gaatcaatga
     6481 gtcggtccac tgcggcgtga ttgatgctca tcgggtgcgc cccctctcgt atataacttc
     6541 tcgttcttcc cgctcttcgg cgagtacagg gtcttatgtt gtgcactgcg caccgcaatt
     6601 gtgcgcacgg caattgaaaa tatccggttg cggaacaagg agtagatgat tccacaaccg
     6661 gatatcggtt tagtcagccg tttcgaacga tgggtccgaa accgcagtgt agtagcgggc
     6721 ggcgtagggg tgctcgtcgg cgtgcaattc aaggatgccg cgacgcgcga gttccgcctg
     6781 atcggccgcc acctgcgagg tgatgatggt cgggcgctgg cctgccacct gcagcttcat
     6841 ccacaggtcg cacaccgaac ctcccgggcc gagcgcactg cggttcaccg ggtttccggc
     6901 ctcgtccaca tcaaccacca cggcgacggt cttcggctga gccggcaatg cggaacggta
     6961 ctgtgcggcg agctccccga acgtacggcc aatcggcttc agctggcctt gctcgtcgaa
     7021 caagcccaga gagtgctcaa agaacgggaa gtcttccatg ctggcgggca catcgtgcga
     7081 gcaccaccac gtcacacccc acaggttccg gcaatccatg gcacgttcaa cggtcttgcg
     7141 gcagaattcc ggcgtgtagt cggtttccag cacattctcc ggcgcaccga cttcctgaag
     7201 ccacaccgga cgctcggaat ccttgccgaa cgccttggcc agctcagcca agtacagtgc
     7261 gtacgacgaa cattcctcac tggtagcgcc gtatccttgc gcgataccgt taaacaccca
     7321 ggagtgaatc acggtcatat cgcccttggt ggcagactgc accggcgtga acgggtgccc
     7381 gtcaatgaac caagtgccat cgttgacgct gtacagtgcg ttatgccctt cgccggcaac
     7441 ggccggaagc agtgcattca gccatgcatc gatctgctgg ctcgtggcgc tcatcttcgt
     7501 aggatgcggc ctgtcagaca gctgattgac ctcgttgccc aaggtaaggc ccttgaacgc
     7561 gggttccttg gacagctcat cggtcatggt cttgaccagt tcacgctcgg ccgcaacggc
     7621 gtccgcatcg gtgaacatgt tgcccgcatg ccaggtgacc agccatgaag gcaggaagtc
     7681 gaagctggac agatgccctt ggaacacgtc cacataggca tccaaaccgt gctcgccggc
     7741 gatgtgcacc atgcggcgca catccgccac gcccttcttg ttgatccaag tgcgattagg
     7801 ttgcagatac ggccagatcg ggaagatgcg cacatggtcc atacccagtt cgctgatctg
     7861 cttgagatcg ttgtcgatgc cgtcccagtc cgggtcgagc catgcgtgga accagccatg
     7921 cgacggcgtg tagttgactc cgaatttcat tggagtatct ccttttttcc agtccgctca
     7981 gcccttgagt ccgccctgtt ccaagccacg gaagaagtag cgctggaagc agcagaagaa
     8041 gacgatgatc gggataagcg agacgagcgc gccggcggca atcagtcgcg gatcttggat
     8101 gaacatgccc ttgagtcggt tcatgccgag ggtcaaggtg tacttgcttt catcggagat
     8161 cgcgatcaac ggccacagga agtcgttcca ggcaccgacg aatgcgaaga tgccgacaac
     8221 ggtcatcgtg cctttgacct gcggtacaca gatgtggaag aaccgctgcc ataggttggc
     8281 accgtccacc atggccgcct cttcgagttc cattggaatg gatctgaagg cgttggtcat
     8341 gagcaggatg ttgatggcac cgcacaggcc aggcagtgcg actgccagca gcgtgttctg
     8401 caggtggatg gagcgaatga tcaggaactg ggagatcatc acgccttcga tcgggatgac
     8461 catggtgccc atgaacatgg tgaacaccag tccgcggcca cgccacttca ggcgtcccag
     8521 tgcgtagccg gccatcgtgg agaaagccac gttgccgaag atggagatca ggcagaccac
     8581 cagcgtgttg agcatgtagt ggaataccgg gacctgcttg aacacggaaa tgtaattctc
     8641 cagcgtcggg gtcttcggca tcaggtaggg aggcacggcg tagacgttgt cgcccttgcc
     8701 cttgaacgac agggacagtt cccagacgaa cgggccgacg agcagcagga acatcggaat
     8761 cagaatcaga tactggacga ctttgccgac agtgacctta tgcgtgttgg tattcatcag
     8821 tcctcacctc gcttttgtac gatctgctgc acgatgagca tgacgccgac gatgacgagc
     8881 acgacgatgg agatggcgct cgcgtaacca gtgcgggcct ggataccggt gccttcgcgc
     8941 ttgataagca tcgacatcgt catatccatg ccgccggggc ctgcggagcc gttggtcaac
     9001 acgtagatct cgttgaagac tcggaatgcg gcgatggtgg acagcatcat gatgagcatc
     9061 atcgtggagc gcacgccggg cattgtcacg tagatgaact ggcggacagg gccagcgccg
     9121 tcgagggagg cggcttcgta aagggattca tcgatgttgg cgagggcggt caggtagatg
     9181 accatgtagt agccgaggcc cagccagatt gtcacgaaca tcgagctgaa gagcacgccc
     9241 catcgatcgg tgaggaatgg aatcggcttg gagatgatct tcaatgcctt gagcacggag
     9301 ttgaccagac cggcggaatc cagcaggttg gtccagatca tgcccaccac aacggcggac
     9361 ataaccaccg gcatgtagaa ggcggtgcgg aagaagccca tgattcggga gttgcccttg
     9421 accagatagg ccagaatcaa cgggaggatc accatgaacg gcactacgca gatcacgtag
     9481 atcgtggagt tgagcagtgc ggtgaggaag tactcgtcgt ggaacagttt gatgaagttt
     9541 tcgaatccga cgaatttgcc cggacggagc agcgtggaat cggtgaaggc aagcctgaaa
     9601 gtgttgagtg ccgcgaggac gatgaaaaca aacacaatca cgaatgcggg cagaacaaac
     9661 aagtaagggg caatgatggt gccgaacttg cgatcctgct tcttgccgtg acgcgtattc
     9721 atcggttcag gccggtcgga ggccgtgcgg gcctccgacc tgtcagcggc gagtgccgcg
     9781 ttgctcactg tgcgagcttc tcgtttgcga actcgacgga cttgtcaagg gcttccttgg
     9841 cggtctgctt gccctgcatg gcgagggcga tctgctgctg gaagttcttg tagccgttgt
     9901 tgtcggtaaa ctcggccgga cgagaaccgt agccgtcctt gacctggtcg agcgtgatct
     9961 tcagagcctt gccctcaagg gtgctctcgt caatgctctt gtagtagtcg tcatccagac
    10021 cacccttagc ggacgggaac acggaggcct tcttgtcgaa ctcgatctgg ttcttctcgt
    10081 tggtcacaaa gcgggcgaag tcgatggcga cgtcggggtg cttcgagttg gcgctgatgc
    10141 cgagcatctc gtaggccacg gaagcggact tgccgtcgtt gctgatacgc gggccgactg
    10201 cgaggttctt gtagaggtcc ggagcgttct gcttaaagcc gtcagcggag taggcggagc
    10261 cggccatgga gaccaagttg ccgcgctgga agagatcggc ggcctggctc caagaggagt
    10321 cgagggcctc cggcgggatg ccgcccttct tgtacagctc aacgaagcgg ctgatctgct
    10381 tgacaccggc ctcatcattg aaggtgtact tgctgtggtc atcattcatg aaggacttga
    10441 cgcctgcggt catcagatct tcggcgttgt aacccatcgt ggtgcccagg tagacatcct
    10501 tgcagttctg cacgatggtg tcgccggcgg cgaagtattc atcccatgtg gtcggaatct
    10561 tgttcggatc aaggccgcac ttctgcatga gctcggtgtt ccagtaggtc gggccatcgt
    10621 tgacatacca tggcaggccg taggcaccct tctcgacgcc attgccctgc atggtcacgg
    10681 acttccatgc gccttcgtag tagtcctttt ccgcatccgg agcctgcttg gagacatcga
    10741 gcagcgcgcc agccttggcc agaccgtaca ggatggagtt gccgccgtcg acgatgtccg
    10801 gcagttcgcc ggccgcagcc tggctggaca gcttgtcttc gtaaccatcg gacggggcgt
    10861 caagccactt gacggtgaca tccgggtggt ccttctcata ggccttgatc agcgcctcga
    10921 aatacggggt gtacttgtcg ttcttgaggt tccaggtctg gaaggagatc tccttcttgc
    10981 cgccgtcatc ccccgcagtt ccgctaccac aggcagccac cgatacgagc atcgcaccgg
    11041 caccggcgat ggcgaggatn nnnnnnnnnc atagaaaaca tacgttttcc tttccttttg
    11101 gttccttttt cgccccgccc tgctgtggac ggagaaaacc taatcgttgt gttccggtac
    11161 agtcacccgt cgcggtgatt gacgtggtat cacaaccttt attcagttct catcgctgag
    11221 actaaaccgt tttagtttcc agccagttat gaacctgtta actaaatcgg tttagtaaga
    11281 atataacaca atcgaaacat gcacgcaacg aattcatccg cgcgtgtcgc attgatattt
    11341 gcccaacgaa atcgtcccct cttctcatac aacaatgggc cgcgcccaac cg
//
LOCUS       DAWBMW010000038        11368 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_1448_length_11368_cov_2.397861, whole genome shotgun sequence.
ACCESSION   DAWBMW010000038 DAWBMW010000000
VERSION     DAWBMW010000038.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 11368)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11368)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11368
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1448_length_11368_cov_2.397861"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1410..1419
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2099..2108
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4620..4629
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9014..9023
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2269 a         3478 c         3342 g         2239 t
ORIGIN      
        1 cccccgagca ttaccccttc ccatggcgac gactcccatc tgctgagccg cattgccagc
       61 ccggccgatg tcaaggccct tgcccccaac gagctcgccg aactctgccg tgagattcgc
      121 accacgctgc tggcatatgg ccaccaacac ggcggtcata tcggctccaa tctgggcatg
      181 gtggaggcca cggtggccct gcaccgcgta ttcgattcgc cgcgtgaccg catcgtgttc
      241 gatgtatccc accaaagcta cgtgcataaa atgctcacgg gccgcgccgc cgcctatctg
      301 gacccggacc gtttcagcga ggtgaccggc ttcaccaatc cggacgaaag caaacacgac
      361 cagttcgtgc tcggtcacac cggtacctcg atttcattgg cttgcggttt ggcgaaaacc
      421 cgcgatatgg aaggcccgaa cagcggcatc ggcaatgtga tcgccgtgat cggcgatggt
      481 tcgctgagtt cgggcgtggc gtttgaagga ctgaacaacg cggctgagca gggcggcaat
      541 ctcatcattg tgttcaacga caatgagatg tcgattgccg gcgatttcgg tggcatgtac
      601 ggtccgctcg cccgcctgcg cgcgtccggt ggcaccgccc agccgaatct gttcaacgcg
      661 tttggcttgg attatcggta tgtggaggcc ggcaacgatg tttccgcgct ggtcgcagct
      721 ttcgaggaag tgcgcgatat cgatcatccg gtggtggtcc atattcatac gctgaagagc
      781 gcaggatatg acggtgagga gactcacgca gatcggcaaa ccgcctcggc ctcaccggtt
      841 tcgttcgact cccccaccgg cgtgcatccc accgataacg tgaaccacag cgaaccgtgg
      901 cattcggatc actgcaacct gtccgatcat gaaccgcatg agggccagtg cgaggcctcg
      961 cattggcaga atccggatgc cgcaatcggc aagccggacg atccgcgcaa gcattacggc
     1021 aaaatggcga tggccgcgct cgaaccgcgt ttcgccgccg agccaggact cgtggtcatc
     1081 tcccccgcca cgccgggctc gaacggcatc acccatgagt tccgagagcg cgcgggcgca
     1141 cattatgtag acacgggcat caccgaatcg catgccgtgg cgtacgctgc cggcatcgca
     1201 cgggcgggtg gcacgccggt ggtggccacc acggcgtcct tcttccagcg cgcctacgac
     1261 cagttcttcc aggagatgag cctgaaccgt tcgcgagtca cggtacttga ttttctgggc
     1321 ggcttgtccg gttcggacaa tacgcattcc ggcgcgtatg acgtgacgat gttctccaat
     1381 attccagggg ttacgatgct ggtgccgacn nnnnnnnnng ccgacttcgg ctcgcgacta
     1441 tctagctgat ctggcatggg ctaccgcgcc ggccggttcg gcggacgccc ctgctggtcc
     1501 ggcggtgatt cgggtgccgg gcgaggcgat tctggcggcg gagcgggatg cgacgctgct
     1561 gccggtgacg ggtgggcaga tcgctgaccg cccccagtca acttcgctgc cagtctccgt
     1621 cagctccgcc agcgggggca tatctgccgc aacagtccgc gggaccgtta gcgtaacggc
     1681 tcagcgtgcc ggtgtgcgtc acatgctgga ttggcgggtc aatcagacgg gctcgcacgt
     1741 cgcgattatc ggactgggca atgcgtatcc gttggcggag cagacggccg ccgcattgtc
     1801 tgccgattat ggcatcacgg caacggttat cgacccgcgc cagtgcacgt cgctggattc
     1861 agacgtactg gagtcgttgc gtgatggcca ccagcaggtc atcacccttg aagacgggca
     1921 gcttgaaggc ggctggggcg agaaagtgac cgcatattat gcgaatcatc atgtctcgga
     1981 cggcagccgt aatcctcatg tgctgaactt cggcgcagcc aaggaattta ccgaccgcgt
     2041 gccgctgggc gaactgaacg aacgttatgg actaacggtg gagcaggtga cagaggcgnn
     2101 nnnnnnnntt attggccggg ccgaagccga acagcaagcc gatcagcgtg gaaaacagta
     2161 cgacgaacag gaacagccac cacggaatca ccgaataacg ggtcacattc tcgccaccaa
     2221 ctatcgcctg ccagagtatg gtccagatag atgttccgcc accggtaccg tcgccggagc
     2281 ccccgcctgc accgctgacc ataccgccag gcatgccgcc gctcatgccg cccgcataca
     2341 acgcgccgac aacgttgatg cccagcgata tcagtccgct cagcacgcag ccgaccaatc
     2401 cgccgaagaa tccgatcgcg ccggcctccg caagaaacat gacgcgaatg tcacgaacgt
     2461 agcagccaag cgccttcata atgccgatct cgcgcgtgcg ctcggttacg gacataacca
     2521 ttgtgttggc gatgccgatg gccgcaacca acagcgaaac cgcgccgata ccgcccagaa
     2581 tcagctgaat cgcgcgcgac tgttcttcca acgacttacg catgtcttca taggaggagg
     2641 tctcgtagcc caacgctttg atctgtgatt cgacttcggg cacggacttg aggtcggcgg
     2701 ccttgaccag cacttgatca taggcggtgg cttttttggc ggcggctgga tcgactttgg
     2761 cggccagttc cttcaacgcc tgcaaatcca tgaccagacc gtcggaggtg gcatagcctt
     2821 tgttgtagtc gcccgccaca atgccggaag cccgcacatc aaaggtgacg ttttccgact
     2881 gacccgcggt gttgccaccc gcaccgcctg tgccgccacc gccgccgtac atgttcatgg
     2941 tgtactggtc gccctgataa ttggtgccgg tggtcagact gatttgcgtg gcgagcggat
     3001 cgaagaacgg atcctcgtcc tggtcttcct cgcactggcc ggtggtctgg tcaaacgtgc
     3061 atgaggaata ctgaccgccg cgtgtgctgg tgcgttgttc gccgttcttc agggtgtcca
     3121 tgaaaccata ggcgaaccat tcgccggcaa gcacctgatc ctttttgacc ggcttctcgc
     3181 caccgacgag cttgtagccc atctggtcga actgggtcat gtcgatgccc atgatctgca
     3241 cgtaatcgta gaggtaacgg ccgcccgcgc cggctctggc cgtcacgtta taagggaagt
     3301 tcatcatcgg cgtcacgccg gatacgccgc tcatcgcgcg gaaggattcc acggccttgt
     3361 cgtcgagctt ggtatcgccc atctcggacg aaccgccacc gcccatcgga ccggcataac
     3421 cgttggcgta cacggtgacg atggacagat cgcccatcga tttgagcatc ttctcgttct
     3481 gctcgttaat gccctggccc aaagagacca tgagcacaat cgagcagcag ccgacgataa
     3541 cgcccagcac ggtcagaatc gtacgggatt tacgccgcca taggttttgt ctgcagagcc
     3601 gcagtatgtc gccgaatcgc atggtcgggc ctttcaggcc ttgtgggatg agccggctgg
     3661 tgcgactgat gaggctgggg tgactggtgt gataggagcg gtgtctgccg cagcaccatc
     3721 cgtcacgccg gtatcggtct tatcatcctt gccttcggac caatcatccc agtcctcatc
     3781 aatatcgttc ttcttggcct tctgcttgcg ccggcgacgc accagcagca caatcaccac
     3841 gataatgacc acggcgatca cgacgatgac cacatacacc agcaccggca atccctgatt
     3901 ggcgttctgt tccatgttcg gatcggccat gccggaatca tccatcggct gcatatcggt
     3961 tacgggtatg gtgagcggga attccttggt cttggtctgt ccgtcggaat cctcgtacgt
     4021 gactttgagc gtggtcttca gctcgcctga cgcgtgcggc gtaaaggcgt aaccaatcgt
     4081 gccggtggcg ccggaagcca cattgcccac gtactgggtt ttcatcgtgg catcgaaacc
     4141 ttcgccctcc atcgtggctt cgacgttggc gatatcgcct ttgcccttgt tcacatattc
     4201 catcgtgacc gtgttttcct gaccggcgat cacctggtcg ggcactacgg gatcactgat
     4261 ttcgaaccgg tcaggctgac tgatcggcac ggaaatcttg acatcggact ggcttgagga
     4321 gcgggcgctg gcgtccacat actcgtatcg gaagttcacg gtcacgggct gggcaccgct
     4381 tttcgcggag gcgagcgcct gcatcggcac gctttgcgtc atggcgtaac cgccccacag
     4441 cgcgtcgacg tagaacgtgt tcgtgcctcc cgcgatggcg aagctctcgc cgccgtccac
     4501 ggtgatgacc atgttcgtca ccgccacctg acccatgttc tggaaggtga agtcgaggtt
     4561 gaaatcggcg ccggcggcca cggaatcgcc gccataggtg aagttggtga cgatgatgtn
     4621 nnnnnnnnna tgatgttcgg caccgggccg gcgattggcg cggaatcgcc gtcgcttccc
     4681 tgagacgtac tgttcgtgcc gcttgtgccg tctgtgccgt cgactgcgta cgcctggtgc
     4741 ggaagggaga tgacggcaag tgccagtgac agcgtggaga gcaacgcgac gatgatggcg
     4801 atggtgcggt gggagaggtg cttcatcggt caactccttg ggttctttgg gcggacggcg
     4861 tggacggaac agttgggcgg gaaaggtcgg tgttgagccg atcgctggtg atgcggccat
     4921 cgagcaaggt gacgatgcgg tcggcgtatg aagccatctg cggatcgtgt gtgaccagca
     4981 cgatggtctg gttgaagtca tgggcgaatg cacagatcat cgtcatgacc tcggttttgg
     5041 ttttagagtc aaggttgccg gtcggctcat cggcgaacac cacctcgggc ttggtcacaa
     5101 acgcccgggc gatgccgacg cgctgctgtt ggcctcccga catctggcgc gggtaatggc
     5161 tgagacgatg gccgagcccg actcgtttga gtacgccacg cgccgcacgt tcgcgctgtt
     5221 ccttgggcac gccgcggaac atcagcggca ttgccacgtt ttcgagcgcg gtcagattgg
     5281 gcagcaggtt gtacgactgg aatacgaagc caagatgctt ctggcggaag gccgccagct
     5341 cacgctcgtc gagtttgctg acgggtacgc cgccgattct cacgccgccg cgcgtcggtt
     5401 tttccatgcc ggccaactgg ttgagcagcg tggatttgcc ggagccggat tcgccgaaaa
     5461 tgcagcagat ctggccttgc gggatactga gattgacacg gtcaagcgcc accaccgtct
     5521 catcctcgac ggggtattcc ttgcgtaggt ttcttacctc gatgattgct cgtggcacgg
     5581 gccttcttcc ctcgttcgtc gccattggcg gacgcgcgat agctggttat tattcagcac
     5641 atctcaagtc agagtatagc cgttatgcag gagacgcacc ccttaagggt gttcccggat
     5701 ggtggtgatg agcatgtgac ttctaatctc aaggtcgaca gttcaggctc cagacggacc
     5761 cagaccggca taatattcaa aagcccctgc ccctcacaaa cgatgaggga ccaagggctt
     5821 attacatcgt gaccccggac gggctcgaac cgtcgacctt gagattagaa gtcacatgct
     5881 ctatccagct gagctacggg gccaaactcg tgtaagtcta gccgtagggg tgtaaaacac
     5941 ctcggcgtaa cgtcggcttg caccggtacc atcggcgata tggggacaac ggagagcaac
     6001 gtagatcacg ccgcatcggg ccagaccgtc gcaggcgata gcggtgagct cactttcaca
     6061 tacgcacagg gtcgttttgc cagtgaaggc aaggattggg aaagcccggt tgtgaccaac
     6121 atggctgcat ccgtctctgc cgatgccggt aatgtccccg ccggcagcat caccaacacc
     6181 ggcaccaaca acaccttcac cagcaccacc gaatcgtcgc ttgccaccct gctgtccaac
     6241 caatgcccgt tcgttatcaa ggccaccatt ttcgagggca atggcaaaaa cgccattcgc
     6301 gaacgtcaca cattcaccgg ctcggtgctg cgccagctcg accaagccag cgccctgctg
     6361 aacggcatca acgaatccgg ccaatacccg gccatagccc tgcgcgaagc attggtcaac
     6421 gcactggaac atcgcgatta cacgtactcc ggccccacac tgatcaatat cttcgattcc
     6481 cgcttggaaa tcgtgtcgct cggcggcctg accgatggtt tggaaatcaa cgatctactc
     6541 aacggcgtat gccagccacg caccccgcgt ttggctgaat tgttcgccga tttgggcctc
     6601 tgcgagaact gcggtaccgg cattcaacgc attatggacg cctacgcgca gagcgcggtc
     6661 agtccgcaac tgcgcgtggg ccccacttcg gtggcaatgg tactgcctat ccctgtatcg
     6721 gccgaggccg cgtcctcgca tcggaccgat gccgatgctt ccgccgcgcc cagcaccact
     6781 gacgaagcac agcatgccaa aatgtactca ttccccacga ttcagcgcat gacggataac
     6841 gccgcagatg ctttggccgg cgcacgcatc atcggctgcg cgccactgca gacgctgatt
     6901 ctcggcagtg gattcggcga acgcccggac ggtgacgatc aggccagtac ccgcccgctt
     6961 gatacggcaa cgctggaacc gtcgcctcaa acgttggaac agttcacgct caatttcctc
     7021 gcgttccgcg gtgtgaagct cagccgtaaa accatgcaga aagcgcttgg cctgaacaag
     7081 gatcagatga cgcagctctt ggaccagctg gagttgagcg gcaagatcga acgtcatacg
     7141 cacgccaagt ccactacgta ttcgctggca catgcctcag gacggtagcg accagggcga
     7201 caacgaccaa atcgtcaaca acccaagcag caacgaccag agcaatacgg cccaaggcgc
     7261 gataaagcag cagctcagtt ctcgaatacg cgatcgcgaa tcatgcgacc ggattcgtcg
     7321 agcgcggtgg agaaacggcc gtagtcggac agaatcacgc ccatgtacag catgtccacg
     7381 agcgcggtat cggccacgcg cgagaaaatc gcattgccat agatcgtgtg cgaatcacga
     7441 cccgtcagca acgtcacgtc ggcccagctg gccagcggcg cgccgatggc attggttatg
     7501 gcgatggttt tcgcgccatg cgacttcgcc aatcgcaacg ctttgaccgt atccgccgaa
     7561 ctgcccgaat gggagaaggc gacggccaca gcgccgtggg gcaggtgccc ggcaccgatc
     7621 tgctgcaggt aagcgtcggt attgagacgg catgtcagtc ccaggtagcc gagcttggtg
     7681 aacagatcca tcgccggtgt caacgaattc tccacgccga aaatgtcgat gaactccgat
     7741 tcggcgagta gggcgaccgc cttgcgatag gccttggggt ccaacgcact gtgcagctcg
     7801 tcgatcagcg ccttcgcgcc atcggccgct ttaccgggaa tctcatcttc gccatcccac
     7861 ggattcagat cgaatccggc cagcggattg aaggtcactt tgtgctcggc ggccggatgg
     7921 cgcaacacgt accgcagttc acggtaaccg ccgaagccga gcttgcgggc gaaccgaatc
     7981 accgtcggct gactgacgtg ggcgatgtcg gccatctggc cgacggtcag gtcggccgcc
     8041 tcctcgatat gatcccgaat atactgggcc acggcgcgct ccgccggacg cagcgacgga
     8101 tacacgtggt cgatgcgctc acgtattccc accgaggatt gcacagccgc gctcctagtc
     8161 gtccgtattt tccattcctg tttcgagggt accggctgta taccggcgaa cagtcgactt
     8221 ggattgaccg caacatgaac ggtgagcgaa ctcttccgtt tacctttccg ctccattccc
     8281 tctacaatca aaaccatggt gcggattagc gtgggaagcc ccacccatca cacaagcggg
     8341 cggctgtacg gcgtgttctt cgaggacatc aaccatggtg cggatggcgg gctgaacgcc
     8401 aacatggtga acaactacag ttttgacggc gtctatctcg accatcacac gtggcgtatg
     8461 gccggcgccg aacgatggcg tacacaggcc gattccctgc gtttctggga tttcatcaac
     8521 tgttccgccg ccagcctcgg cagtgagata cgcgggattc acggtcagcg ggtgaccacc
     8581 gactgccccg ccccgccaat tcacgcacat agccaatacg ctcgcatcac gtccgatgtt
     8641 ccttccgaaa ccggccctgc atatctggaa aacctcggct ataacggagg cggcgacaac
     8701 gggggcgagc ccgcgttctc catcgtcgcc gaccacacct attccgtcag tctcgcggta
     8761 cgtccggttc atgggcgggc caagctgtcc gtcggcgtgg tcgataggta cggtctgccg
     8821 ctcaccagca ccactcgatt gtcgtatatc gtggacgcag atccgcttga cgatacggaa
     8881 gagaccggtc tgaggcagga cgatggtgcc aatgcgcagg attccggcgt ttcgatcagt
     8941 ccggattcgt cggatggtgc catcgccatc ggccgtgacg gctggtaccg gttcaacgcc
     9001 gacgtaaccg gccnnnnnnn nnncctgaac accgattacg gcaagctgcg tatcaccatt
     9061 gacgccggcg aacgcaccac gttcgatctg gatctggtgc agttcatgga tgcggactat
     9121 tggggcgcgg gcgacccgaa atggcgttac ggcaagctgc gccgcgatct ggtggaaacc
     9181 attcaggcgt tgcatccagc gtttctgcga ttccccggcg gctgcatcgt cgaaggtgtg
     9241 acgcccggca atgaatatcg ctggaaggat accgtcggct ccctggccgc gcgacgccaa
     9301 cagtattcga tgtggtcgtt caagatgccg gatggttcca gctattcgca gagctatcag
     9361 atcggcttct acgagtattt ctgcctgtgc gaggacctca aggccaagcc gctgcccact
     9421 ctgttcgccg gcatcgcatg ccagtcgccc ggccgcgatc cgcaccacat ggatatcaat
     9481 tcggccactt tccgcaacaa tgtgattcag gactacctgg acctcatcga attcgcgaat
     9541 ggcgatccgg agtccagctc gtgggccgcg gtccggagtg atatgggcca tcctgagccg
     9601 ttcggcctgg acatgatcgg cgtgggcaac gaaaatttcg gggctgacta cgtggccaag
     9661 tttgacatga tcagcgaggc cattcacgaa cgatacccgg atatgctgtg tgtgatgagc
     9721 gccggactgt tccccttcca gccgacgatg aaacgcagct gggaccatgc gcgagcgttg
     9781 gccgcaaccg attccggggc acatgattcc gccaccggtg acgcgattat cgtcgacgaa
     9841 cattcttacc actcccccga atggtttgca taccaagcgt cacgattcga cgcttacccg
     9901 cgttgcggcg ccggcgtcta tttcggcgaa tattcagcca acggctattt tgccggccaa
     9961 ccgcagaccg aacagggcgc gaacacgtgg aagtcggcac tcggcgaggc cgcgttcctg
    10021 accggttgcg agcgcaattc cgacgtggtg cgcatgacct cctatgcacc gctgctcgcg
    10081 catatcccgg ctaaggggtg ggcgcagaat cttatcgaac tcaatccggc gcatgtgaac
    10141 cccacggtga attatgaggt ggagcggctg ttttccacgc atctgggcga cacgacatat
    10201 gccgtgtcca tcgagcagac cgcgtcacgc cccgccaaac atctgtatgt cagtgccacc
    10261 gggcatgacg gggatgacgc ctgccgttac atcaaaatcg tcaatacctc cgattccccg
    10321 gtggatgtga cgttggaaat cgcgcgcgga ctggccgggc tgggcgcctc gccatcgcga
    10381 ccggtgcgtt tggaggtgac gacgttaagc gccagtccga cggcgaaaac cactatcgga
    10441 taccgcggcg aggcttcggg ggcgattgtg ccggaacgtc gggcttacac attgccgtcg
    10501 ccgtcttcat tgctggcgat gaagatcaag ccgtacagcg tcacgctggt ggcttcgcgc
    10561 tgatcgacat cggccattag gccgattcct ttgccgttag aatcaccctc aaggatgagg
    10621 gggaatcgtg cagattcagg gaataatagg ggctatcccc catagcgaat acaagcacgg
    10681 gagggcagga tggaaaccac ggcggccaga agaccgccgg ccaacgatga aggagtatgc
    10741 ggtatgttta tcaatctgtt ccgcaggcag cgccctgcgc tcgcccggct ggcccgcaac
    10801 tccaacgcct atcgtcagcc gatggttgcg gccgtatcat cgtccgcagt cggcgtatcc
    10861 agcatcacct ctgcagccaa cacagccaac acaggcggcg tatccggcgt atccacgctg
    10921 gatgccacca tcggccgcat ccacgacatc ccgcagtcgg ccatgagccg actgaccggc
    10981 tcccccaccg tatacgcgcg gtagaccggc ccaacccaca acggcggctc agagactcgg
    11041 ctcgatgatc cagctcaagg tcagcaatac ggccaaaaac agaacgttga cggcggagta
    11101 gacggccaga taccggcctt tcttgtgcgg gtattggcgc acgacgttct tgtaagaaat
    11161 aagcgaggcc atcgaggcga tgagcgtacc catgccgccc aaattggtgc caatgatcag
    11221 atcgcgccat tggtcgcaga agccggaaag cagaatcgtg gtgggcacat tgctgatgac
    11281 ctggctggcc agcaggctgg tcagcatggc gcttctctcc agcaggctgt ttaaaaactc
    11341 acgcaccgcg ccgatattcc ccatattc
//
LOCUS       DAWBMW010000039        11311 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_1461_length_11311_cov_2.828802, whole genome shotgun sequence.
ACCESSION   DAWBMW010000039 DAWBMW010000000
VERSION     DAWBMW010000039.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 11311)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11311)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11311
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1461_length_11311_cov_2.828802"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3730..3739
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4043..4154
                     /estimated_length=112
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2215 a         3335 c         3327 g         2312 t
ORIGIN      
        1 ctggtaggcc tggaagttgc cttcgaacca gtaccacttg gccgggttgt cgtcgtcgcc
       61 ttcccacgcg aggatatgcg tggcgacacg gtcgaggaac caacggtcgt gggaaatcac
      121 cacagcgcag ccagggaatt cgagcagcgc gttttcgagg gattccaagg tctcgacgtc
      181 cagatcgttg gtgggctcgt cgagcagcag caggttgccg ccctgcttca gggtcagggc
      241 caggttcaga cggttgcgct caccaccgga cagcacaccg gtcagcttct gctgatcggc
      301 gcccttgaag ccgaagctgg ccacataggc gcgggtcggc acctccacac cggccacctc
      361 gatgaagtcg aggccgccgg acacggcttc ccacaggttc ttgttcggat ccaggccctc
      421 acggttctgg tccacgtagc tgattttgac ggtgtcgccg atcttcagct cgccgctggt
      481 cagcggctcc aggcccacga tggtcttgaa cagcgtggac ttacccacgc cgttcgggcc
      541 gattacgccg acgatgccgt tgcgcggcag cgtgaaggac aggtcgtcga tgagcacacg
      601 ctcgccgaag gccttgtgga tgtggttggc ttccagcacg gtggaaccca ggcgcgggcc
      661 ggccggaatc tgaatctcgg agaagtcgag cttcttgttg ttgcgtgcct cgttttccat
      721 ctggtcgtaa cgctccagac gggccttgtt cttggcctga cgggccttgg gggaggagcg
      781 cacccagtcg agctcgttct tcagtcgctt ggcgagcttg gcgtccttgg ctccctggat
      841 ctccagacgc ttggccttgg tctccaagta ggtggagtag ttgcccttgt acgggtacag
      901 gtggccgcgg tcgacctcgc aaatccattc ggccacattg tccatgaagt aacgatcgtg
      961 ggtgacggcg atgactgcgc ccttgtactg gtgcaggaac tgttccagcc acaggatgga
     1021 ctcggcgtcc aggtggttgg tgggctcgtc gagcagcagc aggtccgggg cctcaagcag
     1081 cagcttgcac agggccacac ggcggcgctc gccaccggag cacacgttga ccggggtgtc
     1141 cgggtccggg cactgcaggg cgtccattgc ctgctcgagc tgggagtcga ggtcccagcc
     1201 gtcggcggcg tcgatctcgg tctgcagctt gcccatctct tccatcaggg cgtcgaagtc
     1261 ggcgtccgga ttggccatct cctcaccgat ttcattgaat cgggcgacct tctcggcaat
     1321 cgggccgaac gccatcttga tgttctcgcc cacggtcttg gtgtcgtcca gcggcggctc
     1381 ctgctgcagg atgccgaccg tgaagcccgg ggtcaggctg gcctcgccgt tgctcacggt
     1441 ctccaggccg gccatgatct tgagcagggt ggacttaccc atgccgttcg ggcccacgac
     1501 gccgatcttt gcgccgggca ggaagctcag ggtcacgtca tcgagaatca cgcggtcgcc
     1561 gtaagacttg cgggccttga tcatctgaaa tacgaattct gccatgttgt gcagttactc
     1621 cttaaaaagg ggttgaaatt ccactgtttc tttagtcttt agtttgtgat ggatacgctg
     1681 tgtgcagcgc ggagcacgcc cccgaatcac ggatcgcggt gcgccgcgtc aggcctgtgc
     1741 cgccctgtcc gcataacgat cgaatccacg ccaaaccacc gcccaccatg gtatcggaag
     1801 ggctggaatg gcgggcgtcg ctatggcttg gaggggtaat ggatgggcct gacattgccg
     1861 tggcgcgacg cgccgggcgg cgcgccgtgg tccagtgatg ctgtagtata tcctcttgtg
     1921 cttcattgag gtacagatgg ccccgtagct cagttggtta gagcgccgcc ctgtcacggc
     1981 ggaggtcacc ggttcaagtc cggccggggt cgccaccaag ctcctgacaa atgtcaggag
     2041 cttttttgtt cttttgccgt atttcgtggt gtagggcgaa atatggggtt ataggccaaa
     2101 atgtgtgtct ggtgtttagt cgtttgatgg ccattcgact ctggcatgga agctgtttca
     2161 gtcgatgccg atgccgtatc gcatggaaat accgaaggtt tcgtgctggc cttggggctc
     2221 tgctcgacac gccgatagtc tgaatgacaa cgtttgctat tgaatgacaa cgttagcgtt
     2281 aacagtgctt ataaatggtg aaaaacgtgt gcagggtcac attggcagaa cgtgcgcgag
     2341 atatgacaaa tcatgaaaaa tacgctatgg gaggtcatgg caactcgtta ctattttcgc
     2401 tgtctcgtac gccacacaag ccttcaagga gtaagccatg accacgctcg ccattgatat
     2461 cggaggaact aagatcgccg ccgccgtctg cgatgaaaac gatagcatca tccagcgctg
     2521 gcgcgtcccc acgccgatgg atgccgacgc catcaaccgg cacatcgccg aggtgtaccg
     2581 cgaggctgtg gccgccggac acaccgatat cgaggccatc ggcatctccg ccgccggcaa
     2641 cgtgagcgcc gaccgcaaga cgctcacctt ctccgccaac atcccggcct ggatcaacta
     2701 cgacctgtcc gagcacgtcg gcgcgctgat cgaccatgcc gtgccggtcg ttgtcgagaa
     2761 cgacgccaac tgcgccggct ggggcgaata cgtgcacggt gccggccagg gcagctccaa
     2821 catggtcgcc ctgaccgtgg gcaccggcct cggcggcgcc atcgtgatca acggtcagct
     2881 gtaccgcggc tccttcggca tggccgccga actcggccac ctgccgatgg tcccggacgg
     2941 cgaccactgc ggctgcggcc tgcgcggctg cgccgagcgc tacacctcgg gcacttcgct
     3001 ggagaacttc gccaagtccg ccgtgcgccg tcgtccgcag gatgccaagc gtctcatgga
     3061 gctgtgtggc ggcgacatct ccaagctgga aggcccgatg gtctcccagg ccgcccagga
     3121 aggcgacgtg ctcggcctgt acgcattcgg caagatcggc gaatggcttg gccgcacgat
     3181 ggccgcagtc tccgccgtgc tcgatccgga cctgtttgtg atcggcggcg gtgtggtggc
     3241 cgtcggcgac attctgctgg agccggcccg ctacaactac cagcgtttcc tggaaggcag
     3301 cgcctaccgc ggccatgcca agatcgtcgc cgccaccgcc ggccaagacg ccggcctcat
     3361 cggtgccgcc aacctggctc tgcgctgacg atgccgctcc ggcaacgggg cgttagtcta
     3421 atggtgagtc atggccggtg ccatggctca ctgccgcgct gatagtgcgg tcgatgagtt
     3481 tccgctgcct gaccatggaa tcgaatttcg ggttctcggc gatttcctca tactcgtgtt
     3541 cggtggtcac gccggcgatt tcggcgataa tctcctcata ggacgtttcg acggttcgat
     3601 gcacgccggt gacgtgcccc tgacgtatca cgacaatgcg gtcggatacc gcgaacacat
     3661 ccggcagatc gtggcagacc atcaccacgg accggccttc ggagcgcagt cgcttgatat
     3721 atgcgagcan nnnnnnnnnc gcgctatggc cactgtctgg cgctggccga cggataagga
     3781 tgcgataggg gagccgaccc gaatcgccga gctcagggtc ttcagcacgg aacgggcacg
     3841 cgagttcatc gaatcgtcat cgcgtatgcc aatctgattg atctccttgc cgaggaacag
     3901 gttcgaggcc acgtccaggt tgtcgcagaa ctcctgcccc tggaacaccg atgcgatgcc
     3961 catgcggtcc gcctccctga tcgagggaat ggtcacctgt tcgccgttga gataaatatg
     4021 gccgaatccc ggctgcagaa atnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnggactt tcctgcgccg ttatcgccca cgatggcgat gacctcctgg
     4201 cgctggatgg acagattcac cgatttcaac gcctcgacga agccgaactt cacacagatg
     4261 tcctccagcc gcagcagagg aggtgtgctc gaattcctca tggtgtgttc cttcttatca
     4321 ctcttctcct ggtaccggtc actggctgac gtcattgcga accgagaact cgatggcgca
     4381 taatgcgccg cctagcgcgc aattgtcgtc cgggtgggat gacacgagta catcgatggg
     4441 tgccacggca tcagggaaca gcatgcgctg caacgcttca ttgaacggtt ggatgaatac
     4501 gtcgccggtc atggccagct tgccgccgag cacaatgacc tccggatcca cgctgatgca
     4561 cagatcagcc gccacctgac cgatacgcac ggccgcgtcg gcgatgatgc gacggcaacc
     4621 ggggtcgcct tcgttggcaa aggacaccag atcatccagc gtcatattgc cgtgtgtgac
     4681 gctgagcaac tgcaccagcc tgttttccgc cacgacggtg tcaaggcagc cgcggttgcc
     4741 gcacgaacag atggcaccca gcgggtcgac ctggatatgg ccgatttcac cggccaggcc
     4801 ggtcacgcca tgcatgattt cgccgttgat gacgatgcct gcgccaaccc cgtcgctggc
     4861 tgagatatat acgaaattgc gtttgcctgc cgccacgccc atacgggatt cgccgtatgc
     4921 cgcgaaattc gcgtcattat cgacgtatac gggaacgttg aacgccgtgc gcaacgggga
     4981 cgtgatatcg acgccgtccc atccgggcag aatgccggga atcgctatgg tgtggtcgct
     5041 ggtggccacc ggtgcggcca cggccacgcc gatgccgacc agctcgctgg cttcggcgtc
     5101 gatgctgctc agggtctcat tgatgagcag catggcgcgt tccagcgttg tgtccgcctt
     5161 gtggcccaac ggcaggggga gcgtgtgctc agcgatgatt gacttcgaga agtcaacgat
     5221 agaaagcgta agatgacgtc gcgcgatcca aagcccgacg ccgaggccct gatgccgtgc
     5281 aagggtcacc agagtggctc tgcgaccgtt gcgaacggtg cttttggtct ccagctggtc
     5341 ttcgtcgacg agctgatgga cgagcgtgga cacggtcgca gtggacagtc ctgtcacctc
     5401 tgcgagttcg acctgtgtca tggcgccgaa cttgtggatg ctggccagca agttggcgcg
     5461 gtttgcctcg cgaagcgagg tctgcgaacc gaaaagcctt gttgccataa gaatccagtg
     5521 tagtcgttac cggcatgagg cacagcgagg cgcagagcgt ggatttttcg gcgtgtcacc
     5581 ttgtgatgcg cgaaaaaatc gccattgcgt tcattggtcg aacgcaaacg acaggaaatt
     5641 cgctttcttg agtttgacaa cttaacttaa tacggtattc tatgagatga acctgaggtt
     5701 attctcaggt tcgggcgacc aaggagggtt cgccatcccc tcgaagatga aggagagcaa
     5761 atgaagttca ctaagaagat cgtcgccgtg gtggcaggcg ttgccatgtg cgcgggcctc
     5821 gcggcctgtg gtggcagccg tagcggccag gcaactggcg gtgatgccaa gatcgaaaag
     5881 ggtgccacta tcggcatttc gatgccgacc aagtccgaag agcgttggaa caaggacggc
     5941 aacaacctga aggccaagct ggagaaggcc ggatacaagg tcatcctgtc tttcgccgac
     6001 gataagccgg cccagcagaa cgctgacatc gagaacatgg tcaacaacga cgccaagatc
     6061 gtcgtggtcg cctccaagga tggcaccgca gtgggccccg ccgttgagaa ggcccgcgat
     6121 gcaggcgcca aggtcatcgc ctacgatcga ctgatcatga acaccgacgc cgtcgactac
     6181 tacgccacct tccagctgga gcaggtcggc gtgctcgagg ccacgtggct cattgaccag
     6241 ctcaagctta aggatggcgc caccggcccg ttcaatatcg agctgttcac cggttccccg
     6301 gatgacaaca acgccaagta cttcttcaag ggcgcttggg atctgctgca gccgtacttc
     6361 gagaagggcg tgctcgtctc cccgtcccag cacggtcagg gtggcgtgac caaggacttc
     6421 accgttgagg attggcagaa gatctccgtg atgagctgga agaccgagca ggcgcagaag
     6481 gacatggaat ccatccttga ctccacctac gcccacggcg agaagctcga cgcggtcctc
     6541 actccgtacg acggcatcgc ccagggtgtg atcaacgcca tcgaatccaa gcgtcccgac
     6601 atgaagccgg gcaccgactc ctggccgtat atcaccggtc aggacgccat ggagatcgca
     6661 gttgccaaca tcgccaagga taagcagggc gagaccgtgt tcaaggacgt gaacaagctg
     6721 gctgacgccg tgtacgacat ggtcgtcgag atcgccgagg gcaaggaagt ctccggcctg
     6781 aacggcaagt tcaacaacaa caacatcgac gtcccgtcca agctgcttga cccccagaac
     6841 atcaccaagg acaacctcca ggatctggtc acggccaact acatcaccca ggatcgcttc
     6901 gacgagctga ctaagtagtc ctcatcaggc aatccgtaaa caaacacaat gacatgcggc
     6961 gcatagccga tatgcgccgc atgtccatga acacagaact gtgacgagga gtggtgacat
     7021 gtcatcaaat gacaccatct tgacgatgga aaacatcacg aagacgttcg gtccggtcaa
     7081 agccctcacc gatgtgaatc tttccgtcga tcgcggcgag atccacgcca tctgcggcga
     7141 gaacggtgca ggtaaatcga ccctgatgaa cgtgctttcg ggtgtgtacc catacggtag
     7201 ctattccggc aaaatcacct ttgatggcaa ggaatgcaaa taccatacta ttaatgattc
     7261 cgaggccgac ggcatcgtaa tcattcatca ggaactggcg ttgagcccgt tcctgtccat
     7321 tgcggagaac atcttcatcg gcaacgagca ggccaagggt ggcgtaatcg actgggacaa
     7381 gacccgtgcc gaggcgaaga agctgctcga tcgtgtggga cttcccgaag acccggatac
     7441 caaggttatg gacatcggcg tcggcaagca gcagctcgtc gaaatcgcca aggcattgtc
     7501 caaggacgtg aagctgctca tcctcgatga gccgaccgct gcactgaacg atgaggattc
     7561 cgcccacctg cttgacctgg ttcgtcagct acgtgacgag cagggcgtga ccagcatcat
     7621 catcactcat aaactcaacg aggtcgccgc catcgccgac aatgtcacca tcattcgaga
     7681 cggttcgacc gtcggtgtga tgtacgtgac gccggagacg ccgctcgacc aggaagaact
     7741 catccgcaag atggtgggtc gtccgctgca caacctgtac ccgaaccacc cctccaatac
     7801 cccgggcgag gaatacctgc gtgtcgagga ttggaccgtg catcatccgc tcgacgcgga
     7861 gcgcgtgatc gtagaccatg cgaacatcaa cgtgcatgcc ggcgagatcg tcggtctggc
     7921 gggtctgatg ggtgcgggtc gtaccgagat ggccatgtcc atcttcggcc actcttacgg
     7981 ttccaacatc tccggcaagg tgttcgtcaa gggcaaggaa gtcaagatgc ccaacgtgca
     8041 gtccgcgatt gacgcaggtc tcgcctacgc caccgaggat cgtaagggca acggtctgaa
     8101 cctgctgcag aacatccgag agaacgcgtc cctggcctcc ctgaccaaga tcagcaaggg
     8161 cggggttgtg gacgacaacc ttgagcgcaa ggaagttgaa cagtaccgca aggacttcaa
     8221 catcaagtgc catgacatcg atgtgaatgt ctccaccctc tccggtggta accagcagaa
     8281 agtgctgctc gccaagtggg ttatctcgca gccggacatc ctcatcctcg atgagccgac
     8341 ccgaggcatc gacgtcggcg ccaagtatga gatctacgaa atcatcgata agcttgccga
     8401 tgcaggcaag gccgtcatcg tgatttcctc cgagctgccc gagctcatcg gtatctgtga
     8461 ccgcatttac acggtgtcct acggtgtcgt caccgacaat gttgagaaga atggatttac
     8521 ccaggaatac ctgatgaagg gtatgaccaa ggaaagggag gtggcagcct gatgagcgcc
     8581 gcaactgcaa cacctgcaaa gaaagaagaa aagaccggcc tgctggccac ggttgccaac
     8641 aatgcccgtc aatacggtat tgtcggcgca ctgctggtca tcgtcgtggc cttcgaggtc
     8701 ctgaccggag gcgtgctgct gaagccgaac tccttcgtgt ccctgattca gcagaacgct
     8761 tacgtgatca tcctggccat tggtatggtg atggtcatca tcgccaccca catcgacctt
     8821 tcggttggtt ccttggtagc cttcatcggt ggtgtgtgtg cactgctgat ggaacgcgcc
     8881 ggagtcaact ggatgctcgc catcgtcatc gctctggtcg tcggcctgat cgtcggcgtc
     8941 tggcagggct tctgggtcgc cgtcatcggc atcccgggct tcattaccac tctggccggc
     9001 atgttgatct tccgtggtct cgccaccgtg atcgtcggcg aatccgtgcc gattacctcc
     9061 ctcgaattcc gtggcattgc ccgtaactac ctgcccaaca tccttggctg gtggggtccg
     9121 ttcgatggtc tgaccattgt gctcggcatc ctgtgcattg tcggctttgc ctggagccag
     9181 ctgcgcaagc gcgcccgtgt ggccaaggtc ggcctggtgc ccgagccgat gagcctgacc
     9241 gtcctcaaaa ttgtgcttgc cgcggtcgtc atcgccttcg tcacctacct gctggcttcc
     9301 tccggtaacg ctgatcaggg tggtaccccg atcatgctgg tgatcgtcgg tgtgctggtc
     9361 ttcatctaca acttcatcct gaccaagacc gtgttcggtc gccacgtgta cgccgttggt
     9421 ggcaaccgca aggccgccat cctctccggt atcaacaccc gccgcgtgga cttcctgctg
     9481 ttcgtccaca tgggcttcct gtccgcagtg gccgctgtct gcatgctttc ccgtctggct
     9541 tccgcaactg ctcaggccgg tatggagttc gagatggacg ccatcgccgc ctgcttcatc
     9601 ggtggcacgg ccgttaccgg tggtattggt accatcccgg gcgctgtgat cggtgcattc
     9661 gtgatgggtg ttatcaacca gggtctgtcg atcatgggtg ttgacaccgc cgtggtgaag
     9721 accatcaagg gcctcgtgct cctgctggcc gtcgccgtgg acatcatgtc caagcgcaag
     9781 aagagctgac cttccccttt ctcctcctcg atgtgggatt cgtgccggtg ccgctggtgc
     9841 ggatcccaca ctgttatctg ccgtcattct tgtaatcaaa ggactttttc cgccatgaaa
     9901 ctcaccgcgc gctccacctc ccgtatgtat gcattgacct gcttggcctg cgttatctgg
     9961 ctctggcagt cactggtcga ggccaataac gatggctcgc tgttcaattg ggccactatt
    10021 gtcttctcgc tgtgcttgct agtggtcatc ggatggagtg gttggaatgc cgtcgccggg
    10081 tggaacgcca aggagactga ggccgccacg gcaggtgcca aggatgatga gggcagcact
    10141 gatcggtagc gtatatacgg ctgttgttgc cggggacata taggtcccgg caaatctgca
    10201 gcgatatgtg cattgcaagc cagggattcc ctggcttttt tatgtcgaaa cggaactcga
    10261 cacgggcgcg caggccgttg ggtttgtcta gagcctgcat atagttactt atggtaatga
    10321 ctattggtaa ctaattggag gttgggccag cgatgtgcga ttcccgaacc gataccaaga
    10381 ccggcttgct tccggtgaat gaggtgcgga gcctcctgga tgtctgctgg aaggccaaga
    10441 ccatcaccga actgatgccg gcattgccga aaggtatgaa accgcgctat gtccacgtga
    10501 tcgacgccgt ctggcatatc aacgaaccga atgggcaaga taccggcacc gcgcgcgtgg
    10561 gcgacgtcag cgcattcctc ggcgtcacta cgccgagcat caccaagctc gtcggcgaaa
    10621 tggccgacct cggactcgtc gtcaaacacg ccgatgccgc cgatcggcgt gcggtgacgc
    10681 tcacgctcac cgaacgcggc ttggatatcc gccgtatcta tgtggaggaa tatcacgcgc
    10741 atttgagcca actgctgggc ggattgacct cggaccagtg cgaaaccacg gtacgcacct
    10801 tgaccgaggc tctgcgactc atgcaggaag acttccaacg tcgtagtcag aatatctgaa
    10861 tgaatggagg agagaacaag atcgtgactc attccgcaac acagctgcct aacggcggca
    10921 agcaaccggt ctccaacaaa ctcgtcatcg cggtcgtctc gctcgcgatc ctgaccttcc
    10981 tcggcatcct atcggaaacg tcactgaaca tcgcctactc cacgctgatg gaggagttct
    11041 ccatcggcgc ttcggtggtg cagtggctca ccaccggcta tctgctcttg ctgtcagtat
    11101 cgatttcgag ctcgccgttc atggtgcgca agttcgccac caaaaccctg ttcatcatgg
    11161 ccgtcgtgat cttcgccgcc ggcacactgc tgggcgcgct cgcggtcaac ttcccgatgc
    11221 tgctcgccgc ccgactggtc atgtcgctcg gcaccggcat ctccttgtcg ctcatcacca
    11281 gcatcattct ggaaaagcgc cgcttgaaca g
//
LOCUS       DAWBMW010000040        11206 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_1476_length_11206_cov_2.480495, whole genome shotgun sequence.
ACCESSION   DAWBMW010000040 DAWBMW010000000
VERSION     DAWBMW010000040.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 11206)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11206)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11206
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1476_length_11206_cov_2.480495"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2808..2817
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2228 a         3212 c         3406 g         2350 t
ORIGIN      
        1 ccagaaggcc gacccgagcc acaaggacga ctacgccaag ctcaacaaag aatggcacgc
       61 caaggaagac cagcttgagt ccaagatcaa ggagacttcc gccaagaccg agggcctgcc
      121 gtacgccgcc accgaatccg tggcctggta cctggccgac gacctcaaga tgaccgacgc
      181 cactccgaag ggctacgccc aggcctccgc caacgagagc gagccgaccc cggccgacat
      241 caaggacttc caggacaccc tgaaggccgg cttcatcaag atgttcgtct tcaacagcca
      301 ggaagcaaac tccactattg accagatcac cggtgccgcc aaggacgcca acgtgccgat
      361 cgtggagctc gccgagcaga tgccgaagca gtacaccaac ctgctcgact ggatgagcgc
      421 actggtcgac cagttcgccg ccgccgtgaa gtaatgcggc agtagccgtc aatccgcaac
      481 agcgataagg ggaataatca agtcagatag cgaggcgttg tctaggggag aacagaacac
      541 ttcgctatca gggataggct ccttatatat cagggtgctt cggggatgtc ccgaggcacc
      601 ctttgcgtta gggtgaatag ttaggaaacg gtaagaaaaa tagtggaact atggattttc
      661 caaggttcag cggcaacaat atgcccttga aaagtcacat acggaaacag gcgaatgagc
      721 gagaacacta gtaataccac cacagtgaaa ccccatttca agctttggac caaaaaggtc
      781 gaagccaatg aagtcacgcc ccaacacccc cacaagccct tccccggctg ggtgtacggc
      841 gtgctcttcg ttatcttcga catcattgtc gtgacggcgc tcgaagtggg cgtttcgtcc
      901 agctccaccc gtgtgcagct gtccagtccg atcgtcggat tcggcttcgt gagcaagatg
      961 tggaccgacg gcaatttcgt attcgtgctc aacgtgatac ttgtcgcgtt gctgtacctc
     1021 atgttgctga tgctgttcaa ccggttctgg acggcaagca tcgtgattct cgcggtcgga
     1081 atcatcgtgg ccgccatcga acatttcaag gtcgagatcc gttacgaggc cattctgccc
     1141 gccgatctag gcttccttgg ctcgaacacc ggcaacatgc tcaccttcat tccggccggt
     1201 gcgcatgtga ccatcctcgt ggcactcggc gcgttcgccg tactgctcgc gctgattctg
     1261 gtgctgcgtc atttcgatgg gcgcaaaggt cgcatgattc gtacggataa cctgagcctg
     1321 aacctcacct cccgtctgat tcttctgctg ctgccgattc tggtgttcgc gttgtactgc
     1381 attcatgtga gcaccaccga ttcgctggcc aacaagttct cccgcgcctt gggggacacg
     1441 ccatccatgt gggattccgt gtatgacgcg cagcgcaatg gtccgctcgt ggccttcacc
     1501 cgccagctca acccgaagat catggacaag ccgagcaact actccgagga gaccatgaag
     1561 aaggtcgccg ctcgctatca gaaggaagcg gagacaatca acgcctcccg caccaacaac
     1621 ctcactgatt cgacagtggt gtacgtgttg tccgaatcct tctctgaccc gagccgcgtg
     1681 ccgggactga agaccaataa ggattcgatg cccaacatcc gcaagatcaa ggcgggcacc
     1741 acctccggcc tgatgctctc ctccggctac ggcggaggta cggccaatct cgaatacatg
     1801 ggactttccg gcctgagcat ggccaatttc gagtcctccc tgtccagccc gtaccagcag
     1861 ctggtcccga gccagcattg gactcccacc atcaaccagc tgtggggcgc gcccgtcaac
     1921 tcgctcggct accacccgta cgaatccagc atgtactcgc gtgccacgaa ctacaagaag
     1981 ttcagcttct cgcacttcta cacgttgacc ggtccggacg tgatcaagta tcaggacaag
     2041 attgacgagt cgccgtacgt ctctgataag tcttcctatg attctgcgct ggaaggcatc
     2101 aggtccggca agacgaacaa gttcattcag atcattacca tgcagaatca catgccctac
     2161 cacgagtggt atgagaataa cgactacacc gccgaatcca ccacgggtac gccgctgggt
     2221 gatgatgaac agcagtcgat tgagacctat caaaagggtg tggagattac cgatcaggcc
     2281 actcaggagt tcctgaatga actggatgcg ctggataagc ccgtcaccgt ggtgttctat
     2341 ggcgaccact tgccgggcat ctactcgtcc gccagcgagg atgacaacaa ctccttggcc
     2401 ctgcacctga ccgattactt catctggtcg aataaggctt ccagctccca aggcaataag
     2461 gcaagcgatg cagcctactc ctcgccgaac ttcttcgtgg cgcaggccgc cgatcacatg
     2521 aacgccaagg tcagcccgta tctggcgttc ctgaccgaaa tgcattccaa gatcgccgcc
     2581 atggaaccgc ctgtggtcaa caagatccaa ggctgggatc gtatccccga aggccagaac
     2641 atctatcttg atcagaacgg caacccgatg tccaccgatg acttcgacaa ggaaaccaag
     2701 cagttgcttg ccgactacaa gctcatccaa tacgacatca ccgccggcaa aaactacctc
     2761 aaagacacgg actttatgac gctgccgtaa agcagacagc ctagtagnnn nnnnnnngcc
     2821 tagtaggctg tctgtaggca gcgtgcgagc cgacagacga gccagcaagg cgcggggcgt
     2881 agcccgtcca aaattgcagt agcgctaccg taaagcaggg ggtgctttca tgacgaacca
     2941 gactttcgaa caactgaaga agacttacga atccggtcgt acccggccgc tggcatggcg
     3001 ccgcgcgcag ctgaacgcac tacgccgtct ggttacggag aaccgtgatg cctttgtctc
     3061 ctcggctatg gctgacttgg gcaagcccgc ggctgaaacc gtgctcatgg aactgaacct
     3121 ggtggctggt gaagcacagt tcgtgcgcaa tcgtctgagc ctttggaccg cccggcatcc
     3181 caaagcgatg cattggatgt tgcagccggc tgccggatgg acgattgccg agcccaaagg
     3241 cgtggtgctc atcatttcac cgtggaatta tccggtgctg ctggccttgg aaccaatggc
     3301 tgatgcgctc gctgccggta acgccgtctg cttgaaaccg tccgagcttt caccgaatac
     3361 gtccaagctc cttgctgagt tggtgccgca ataccttgac tccgaggccg tgcgcgtggt
     3421 cgaaggcggg cccaaggaaa ccggcgaact gctgaaatgc ccgttcaacc acatcttcta
     3481 taccggtggc ggccacgtcg gcaaaatcgt catgcgcgcg gcggccgagc atctgacgcc
     3541 ggtcacgctg gaactcggcg gcaagtcgcc atgctttgtg gatcgcactg cggacatcaa
     3601 tgtggctgca cgccgcattg cgtggggcaa attcaccaat gccgggcaga cttgcgtggc
     3661 acccgactat gtgctcgcca cgccggatgt ggccgaggct ctggccgagc gcatcgccgt
     3721 agccatcacc gaattctatg gcgaagatcc gaaggcctcg cctgattttg gtcgtattat
     3781 caacgatcgt cattttgaac gactgtgcaa gctattgccg gtcggcaccg tgccgcctga
     3841 ggagccgagc tcgccgctgg tgcaggtggc atcggcggta ggcgcggcca tggatatggt
     3901 tgggcggcgc ttcaatgccg tgactactgg gcgcggtggc gcgggggaga tggccggcag
     3961 cgcgaacaat gcggcgaagc cgacggccgg ttcgaatatg gcagccgctg atgatggtgc
     4021 gactgcctcg gccgctgttg agccgagcga gattcacaag gtgcccggcg tgttcgacct
     4081 ggccggtcga atcgtttgcg gcggcaaggt cgatcgtgcc gcccggtata tcgcgcctac
     4141 cgtgctgtac ggtacgtcgc cagatgcgcc agtaatgaag gaggagattt tcgggccgat
     4201 tctgccgatt ctggtggtcg aggatgccga gtccgcaatc cgattcatca acaagcggtc
     4261 ccggccgttg gctgcatatg tgttctcgcg cgatcacgag gttcggctga ctttcgaacg
     4321 tcagaccagc tccggagcct tgggatattc gctgccgttg ggccatctgc tgtccagccg
     4381 gttgccgttc ggtggcgtgg gcgcgtccgg catcggcacc taccacggca aagcaggctt
     4441 cctcaccttc agccacgtca aaaccgtggt taccaagccg cagttccccg acactctgcg
     4501 cctcgtgtac ccgcccttca atgaagccaa ggccaaactg ttcgacatca tcgccaaatt
     4561 cagctagcgt gatatcgccc ggtagccacg tcaatcgatt gacatgtctg ccaggccaaa
     4621 aatatgcagc agatgcgccg cttctgttac ttcagggctt tgaagccgat gtcgtggcgg
     4681 taaaacagtc cgcgcagctc gagcttgtcg atgattgcgt agaccttgtc ctgggcggac
     4741 ttgatgtccg gagcggaggt ttcgacgagc agcacgcggc cagagttggc cacaaggtta
     4801 ccgtgctcgt cagaggcgac accggcgtag tagacgtgcg aatcctcgtc gacttcgatt
     4861 tcgggcacac gggcaccctt gatgacgttc tccgggtagc cgttggaggc gatgaccacg
     4921 ccgagggtgg cgttggactc atcccaggtg aattcggggg tttcgccgtc gagaatggcg
     4981 gagatgccgg caccgaggtc ggaggtgagc ttcgggagca cgacttcggt ttccgggtcg
     5041 ccgaagcggg cgttgaattc gatgaccttc gggccgtcgg cggtggcgat cagaccggcg
     5101 tacaggatac cggtgaatgg cgtaccttcc tcggccatgc cttcgacggt ggggcgcacg
     5161 attttctcga tggcctcgtc gaccacggac tgcggaatct gcggcaccgg gctgtaggca
     5221 cccatgccac cggtgttcgg accctcgtcg ccatcgtggg cgcgcttgtg atcctgggag
     5281 atgggcatcg gccagaaatc ggtgccgttg acaaagctca tcagggagaa ttcctggccg
     5341 tccaggaagt cctcgataac gaccttggca ccggcggagc cgaagcgatg gtcgatgaag
     5401 atgtcttcga gtgcgcgctg ggcggtgtgc tcgtccatgg ccacggtcac gcccttgcct
     5461 gccgccagac cgtcggcctt gatgacaatc ggggcaccgt gctcgtgtac gtagtcctgc
     5521 gccatctcga gatcgctgaa ggtcttgtat tgggcggtcg ggatgttgtg tcggtccatg
     5581 agctgcttgg cgaagtcctt ggagccttca atctgggcgg cggccttgga cgggccgaac
     5641 gccttgatgc cggcggcggc gaaatcgtcc acgatgcctt cgatcagcgg gacttccggg
     5701 cctacgaaca cccagtcata accattgttc ttcacgaatt cgataagtgc ggcgtgattc
     5761 gactggctca gctgcgtggt gtggatgccg tccttgagca tgcccgggtt gccgggagcc
     5821 acggtgactt cgtcgatgct cggtgccttg agcagcgtgt aggcaatggt gtgctcgcgc
     5881 gcgccggagc cgataaccaa tactttctga cccatgttgt tcctttcctg cggaaagcga
     5941 gttacttcag tacgatttgc gtgccggtgg tgtcggctgc gatctggccg agcacgtagg
     6001 cgggctcgtt gttgtcggcg agcagcttca gcgcctcgtc cttgcgagcg aggtcgatgg
     6061 ccagcaccat gccgatgccc atgttgaaga tgttgtacat ctccttatga tcgatgttgc
     6121 cagccttctc cagcacgtcg aagatcggga gcacgggcca ggtgccgagc tcgatttcgg
     6181 cggccaagcc gtccggaatc atgcgcggga tgttctcgat gaagccgccg ccggtgatgt
     6241 gggccacgcc tttgaccacg cccgccttga acaggggaga gagggccttg acgtagatct
     6301 tggtgggggt gagcagcacg tcgccgagct tttcgccgcc cagctcgtcg agttcggtgt
     6361 ccacggtgta gccggcctgc tcgaacaggg ccttgcgcac cagggagaag ccattggagt
     6421 gtacgccggt ggagggcagg ccgatgagca cgtcgccttc agcgatggtg gaaccgtcca
     6481 cgatggcgga cttttcggca acgccgaccg agaatccggc gagatcgtat tcgtcttcgt
     6541 cgtacatgcc cggcatttcg gcggtttcgc cgccgaccag cgcggaaccg gcctgtacac
     6601 agccgtcggc cacaccggcc acgacctgct caagcagtgc cgggtcgttc ttgccgcagg
     6661 cgatgtagtc aaggaagaac aacggctcgg cgccctgggc ggcgatgtcg ttgacgcaca
     6721 tggccacgca gtcgatgccg atggtgtcgt gcttattggc cagcttggcg accatgagct
     6781 tggtgcccac gccgtcggtg ccggagatga gaaccggctc cttgtagccc aaggaggcca
     6841 gatcgaacag gccgccgaag ccgccgatgc cgccgaccac gccggggcgg ttcgtgcggg
     6901 ccacgtgcga cttgatgcgc ttgacgactt cgtacccggc ttcgacgctc acgccggcgt
     6961 tctcatatgc ttttggcatt tggttggcct ttctacttat tttggctccc ctctggaggg
     7021 gagctgtcgg caaagccgac tgaggggagg caagcgcctc tggttcaaag aattgttatt
     7081 gagtggattg gtggtgagct attcggcatc cggctgcacg gtgttgtact cgttgccgat
     7141 gtacttgctc ttgtacagcg cgaattcagg cagacgcaca cggtcctcgg gcgtgaggga
     7201 cttcaggaag tcggcctcgt agtcgtcgag cgcagtgggg tagtcgccgt tgaagtaggc
     7261 gacgcacagg ccaccgtatg gggcgtcggc gttcagtccg atggattcga ccagaccgtc
     7321 gagcgaaagg taggccaagg aatccgcgcc gatgtattca cggatttcct ccacgctcat
     7381 cttggctgcg atgagttcct tggtggtgga gatatcaatg ccatagaagc aggggtactt
     7441 cagcggcggg gagctgatgc gcatatgcac ttcagcggcg ccggcttcct tgagcagctg
     7501 cacgatgcgc ttggacgtgg tgccgcgcac gatggaatca tcgatgacga taacgcgctt
     7561 gcctttaacc acgctgcgca cagcagagag cttcatacgc acaccctgct cgcgcagttc
     7621 ttgcgtgggc tgaatgaagg tgcgggccac atactggttt ttgattagac ccatttcgtt
     7681 gggcaggcct gctgcttccg catagccgga tgcggcggac aatgaggagt tcggcacgcc
     7741 gataaccata tcggcctcca ccggggattc ggcggcgaga cgtgcgccca tgcgcttgcg
     7801 ggcggagtgc acgttgacgc catagatatc ggaatccggg cgggcgaagt agatgtactc
     7861 catcgagcag atggccagct gcgtgttgtt ggtgtactgc acgatcttgt agccgtgatc
     7921 gttgaccacc acgatttcac ccgggcgaat gttgcgcacc agctcggcgc cgacgacatc
     7981 cagtgcgcag gtttcggagg cgagcacgta ggcgccgttc ttcatcttgc cgagggagag
     8041 cgggcggaag ccgttcgggt cgagtgcgcc gatcatggcg tcttcggtca tcagcaggta
     8101 ggcgaaaccg ccatgcaccg tgttgagcgc ttccttgagc ttgtccataa aggtgcgctg
     8161 catcgaacgg cgaatcaagt gcatcagcac ttcggtgtcg gagttcgaat ggaagatggc
     8221 gccttcgtct tcaagcttgc ggcgcagtga cgggcagttc gtcaggttgc cgttatggca
     8281 cagagccacg tcgccgtcat ggaagcggaa gatgaacggc tggatgttgt ctgtggtgcc
     8341 cgagccggcc gttgcgtaac gcacgtggcc gatggcgcaa ttgcccttca gtcgttcgat
     8401 ttcgcgctca tcgccgaaca cctgggtgag caggccgagt ccgcgatgac cgatgagatg
     8461 accgttatcg ttggagacga tgccggcacc ttcctgaccg cgatgctgca gcgcgtgcag
     8521 gccaaaatag gtgagtcgtg aggcgtcggg gtggccccat acgccgaaga tgccacattc
     8581 ctcgtgaatg tcttcgagtt cagccgacaa gccaaagctc ctttttcaaa caggggatta
     8641 actatctatc aaccctacca atcggcggct acatcgcgtg ttgtggggtg gtggaacttg
     8701 ttggggtttg cggacgcggt ttaagcacca gatcagtcaa gtatgaatag tggcttagga
     8761 ttttggcctt tcgctgtttg tggtgggtgg ttgtggtgtg gggtgatgcc gacgggtagg
     8821 ttggaagcat ggatacctct tacctgttca ccgccgcatt acctggtgct ccactttgtg
     8881 ctcaagcgtg ataccgaatt gtcggtgtga cgtgatcggt tgactttcgt caacgcaatt
     8941 tgaaaggctc cgacggtatt atccgtcgga gcctttcgtc gtatgaatcc ggtgcttgtt
     9001 tttcggcttc gtctcagtgc ctgaaatggc tgaagaactc gatggtctcg ggatcctgcg
     9061 actcgtccat cacctcagcg ggtgtgccgt tctcggcgat gacgccgtga cggagcagta
     9121 cgatgcggtc ggccgcttcg tgggcgaagc tcatttcgtg cgtggccatg aggattgtgg
     9181 tgccttgggc ctttaactcg gcaaccatgt tgagcacttc gccgaccagc atcggatcaa
     9241 gagcggatgt gatctcgtca agcagcagca gttccgggtc ggtcatcagt gcgcgcgcaa
     9301 tcgccacgcg ctgctgctgg ccgccagaga gctgatcggg gtaggcactg gctttatcgc
     9361 gcatgccgat gcgttcaagc agttccatag cgcgggtttc ggcgcgatcc ttgctccagt
     9421 ggtgaacctt gattgcggca agggtcacgt tcctgagcac cgacatgtgg gggaagaggt
     9481 tgaactgctg gaacaccacg ccgatacgtg cgcgaatctt gtcctggtcg gcacgcgggt
     9541 cggtgatatc ggtattgccc agccaaatct ggccgtcatc gacctgttca agcaggttga
     9601 cgcatttcat caatgtggat ttgcctgaac cggaaggccc cagcaaagcc accacctcat
     9661 gttggtgaat gtcaaaggaa atgccacgca gcacctgcgt gtttccaaag gatttgcgca
     9721 cgtcatcaag acgcagcacc gtcttgttgc tcacggggga gtcactcgcc tcggtggtga
     9781 tattggtcat ttcgctcata ccgttcctcc gctgagttcg cgcttacgca gacgttccgt
     9841 gtaccagtcg ttcagcagga tgaacggcac gctcatcagg atgaacagca ggctggcgac
     9901 cacatacggc gtgaagttgt aggtgctggc cacgtcgatc tgtgcggcgc gcacggcatc
     9961 caccgcaccc agcacggaaa tcaggccgac gtccttttgc atggagatga agtcattcat
    10021 cagtgcgggc gccactttgc gcaatgcctg aggaatgacg atcagtcggg tcgtctggcc
    10081 ggaggtcagg cccaacgagc gggccgaggc tcgctgagaa ggatgtacgt cgttaaaacc
    10141 ggcgcgcagc acctcggaga cgtaggcgct gtatcccatt accactgcaa tggtgccgag
    10201 taccgaggga tcgatacggc cgaacaagcc taggccggga atgccgaaac cgattagata
    10261 cagcacgacg ataataggca cgccgcgcat cacagtcgtg tagatcaatg ccaggatacg
    10321 cagcgggaac agaatagggg agcggctggt gcgcaccagc gcgatcagcg tgctcagaat
    10381 cgccacgccg accactgcga cgagcagaac gcggatgttg agccataggc cgtcaagcac
    10441 atggggaaac gatttgacga agtattcgcc ggagaagaag gtctccttga ctcgtggcca
    10501 gccgggggag agcttcaagc cgatgacgat gaccaccacg aacacaatgg tgctgatcag
    10561 actggtaatc gtcgattgaa tttgctgatg aacacgatat tggcgtcgtt cgagctcgac
    10621 ctgacttacc tcgtgtccgt caggggcatt gtgctgcctg gtttcggttg gtgctgcggt
    10681 catgtgatga atgtcgctcg cttcctgaat gccgggatgg ccgggtgcca caggaaaatt
    10741 cccgcagcgc ccggccaagg tgttgtgacc ttatgatgta agcctacttc acttacttct
    10801 tcagttcggt caggtcggtg gtgtactcgg ccagccactt gtcctgaagc ttcttgatgg
    10861 tgccgtcggc gttcaggtca tccactgcct tggtcacggc tgcagtcagc ttggaatcct
    10921 tcggcagcac gatgcccatg ccgctcttgt cttcggagtc aggcagacgg ccgagcacct
    10981 tggcgtcctt gacctgctcg gactgcacca tgtacacgca ttccacggtg ctggtgacca
    11041 gtgcgtcgat ctgaccggcg tcaagtgcct gagccagtgc ggcatcatca ttgaacgtct
    11101 ggatgtcgtc cttgatgttc gccttggcgt agtcgtaggc aagggtgccg accatgacgc
    11161 ccacggtcgc atccttcaga tcggatacct tggtggcgtt ggcgaa
//
LOCUS       DAWBMW010000041        10934 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_1515_length_10934_cov_3.185219, whole genome shotgun sequence.
ACCESSION   DAWBMW010000041 DAWBMW010000000
VERSION     DAWBMW010000041.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 10934)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10934)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10934
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1515_length_10934_cov_3.185219"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2058 a         3348 c         3294 g         2234 t
ORIGIN      
        1 gttgcatggt gaccttttcg tttattaagc cgcctgcgcg cggctcattg cggcgacggc
       61 gcatggcgga tgccttgcgc cgactcactg ctgcgttgtg cgtgggactc gcagtgctgt
      121 ttgggcttga agccgtattg gccgtggtgg agaccgagcc gatggtggtg gccgcccgct
      181 cgattcgacg gggtgacatt attctggccg ccgacgttca gttggctgat atgccggtat
      241 ctgcggcagg cccgtcttgg acaggcaata ttgaggatgt ggtgggcaag gtagcgcaaa
      301 tcgatatcga agcagccgat cccatttcca cgcatatggc cagagatgcg cccgtagcac
      361 cgaccggcac tacggtgatt gaagtccgct tggccagtag cgtcgacgat gtattggccg
      421 gcgatcaggt gagactggtt tccgccgtcg gttgcgaagg cgctgattgc acgctggccg
      481 agaacgccac ggtgatgaat gtcggcaaac cggatgcgat tggtgcattg ggaggcaacg
      541 accgactggt gtcgttcgca atgccgccgg aggccgcggc caaggttatg gagctgcaac
      601 aggccggagc gattatggcc gttgtgcggt aggaagcgga atcggccttc gtccgatact
      661 gtgttttccg cagccgacag gctgcggaaa acacagtgtt ggggcgcaca gagtgcgccc
      721 caactcctac tcgcgctcgt taaaaacggc ccaatgcggc ggcagttcgc cgaggatgcg
      781 tttgtcgtcg tcggattgcc gttgttggtc ggtctgcatg tctccgggct cgagtttgac
      841 accgtcagcg tcaaactgct cagtgccctt catcaccacg cgcttgtgct tgcgcggtgt
      901 gcgccgtgta gccgagtatt ccgccatagt tattcaacct cgcgatagat ctcaccgata
      961 cggctgacga tacggtcgac ttccttatcg gggttcacga aggcggcgac actccagacg
     1021 gtcatcacat tccagcccaa ggaaatgaga tcctgtgccg acaaacgatg acgcctacgc
     1081 gtggattgca cggacataaa gttcgcgtca tcggtctgca cggccaaggc aaacggcttg
     1141 tccttcaatc ccaccacgag cggaatcttc acgcccttgt cgaatccata atcaacagca
     1201 gcgttcagtc ctcgcgcgcg caacctgtcg gcgatatcgt cgaacagcac gttgccgcct
     1261 gccgcatcac gcaacggaag caccggacgg tcgtcaagct gttcggccca cgtcaacatg
     1321 gtcttcagga accgaggacc ggattggtgc agtcgttctt catccaaatc ttcggaacca
     1381 aaggccgaca caatatccaa gctgtggtcc gccaatgcca gggcatccag caacagtccg
     1441 cgaccgcctg tgctttcgag cggaccgaac tgctgaagca gacgtccgtg cgtggttttc
     1501 gcatagcaca tcgacagaat cacgtcagtg gcacgacctc cggccacgtc acgcaaggaa
     1561 accaaccgca catggcgcag gaatcggccc atcgccttgg atttgcctgc caacgacttc
     1621 aattcggcac ccaagcgtgt gcggaatgcg tcggtcaaca tcacgaccac aagtcgatag
     1681 ctggaaggaa ccacggcaaa accggcggca cgttgcgtaa tcagacgcac cacttcatca
     1741 atctccggct ggctggattc caccagacca gaagacataa ccggcacacc gctcgcttcc
     1801 acacggtgga atcgcacctc accggcagcc ggctcggtgg ccacatcata gcgaacagga
     1861 ccatatccct cggattcaag gaatgcggac aggcgcggcg cacggcgaga cggggcagca
     1921 tccacctcga tatgaggcaa cgtgtcgatc agctgcttga cgctttcgct ggttaccgtc
     1981 gcgcagtggg cgatcaccac cacctgctgc acgcggccga tgatggacag caactcgata
     2041 gcgggaatgt gcgcgcacgc atcgagaatc gccacatcag ccagcacacc cggctcggtg
     2101 agtgcggcga gcgtacccgg cgcggccacc agaatcggct tggccgcggc cagaatctcc
     2161 ggatgatcac ggcgaatcct gctcagcgac acactggcgc gccctgccag cacggtgtgc
     2221 atctggttgg cttcctgagt gcgggagaac agcatgtcgc acaggcggcg catggattcc
     2281 tgcgacacca tcggtccgat ggagcgcacg tgctccacat cgacctgcgc aaagcggtcg
     2341 gatgcggcct gcagcgcggc accatcctgg ttggagatga tggcggacga acgcacaatg
     2401 tcctcaaaca cggtggtcca ccaggccagc tgcacttcgc cgcgcacctg ctccacgctc
     2461 actcggcgag cgttgagatc ggcgaccagt tcgttcagtc cggcagaggc gaattcctgc
     2521 tccagcaggc aacgctccgg caaggtgtcc agtgccttgc gatcatccag cagtgctttc
     2581 aggcgtgctt ccaccttctc gaaatcggcg gtttccagat tgccaccagc gggtgtggtg
     2641 gaaagcacgg tatccagcgc ggtcatattg ctgaccagcg cctcttgggt ggagatgatc
     2701 tcgtcgagct tgctgggcag caccggccaa ccgccgtgag gcacgaactg atgccactgc
     2761 tccccttgct tggcgactac cttcaacgct tcgtgcaagt cctcgacctg cgcgccgacg
     2821 cgcagcagat ccttggcttc cttgatgtgg cggcggcgct cccagaatcc catcgacgtg
     2881 ccctcggcct tgcgctggct cttcggcttg gtggcctcga tcatggagct gatgtctcgc
     2941 tcgaaaatct ccggctggaa cacgtcaagc actcgtcgca gattcttcaa aaccgtaacc
     3001 tgacgctccc actcgcgggt ggtgggagga accgggaatc cgcaggtctg cacggtgcgt
     3061 gccacctgct cacgggtggc gggcaggaag cgacgcagca aatcgtccac gcgctggtag
     3121 gcggttactg cctgttcttc agtggtgatg gacgccttgt accatgcggt gtcttccggg
     3181 ccgatggtgt attcgccgag ctcgccggcg cgttccatct tgccgatcca tgtgtcaata
     3241 tcgttggcga tgctcagggc acttgactcg tcaagtcgaa cgtgggtggc agggtgcgtc
     3301 gggagcacgg agatacgggc cagattctga atggtctcgt atgcggacac attccactta
     3361 tcgttgcccc cgtgcaggtc gcccagatag cgggtcaagc gcgagcgcac gcccacgagt
     3421 tcgtcggcca gctggtcgaa tcgctgggtg gcgactccgg gctggaatcc gactgcggca
     3481 atcagctgct tatcaagcgc ggcgttggcg tgttcatcgg atacatccag tacctgcgcc
     3541 ttcatctcat tggcggaagc cgtctggatg aacagacgct tctgctcggc cacgccgggc
     3601 acgtacagca cggaacgacc attcatcagg cagcgcgatg cgatggccac agcctgctcc
     3661 gcggtgccct tcgggaaggt gccgtcgacc acgatgctat ggccattggc agcaagtgac
     3721 gcggcatatc gcaccgtatt gtccacatcg ccgacttcgt actcggcatg cgggtctacg
     3781 tcgaaaggac tgtattgcgg aatattggca tctttgagcg cggtgcgggc ggattcgtcg
     3841 ccggcaagcg cgtccaacag cacattgccg gtggggccgt tctccagctg gtcgatgaac
     3901 tgtcggcttt cgctgatcat ctgcgacgag gggtccatga agcatccgag cacaatctgg
     3961 cgctcgatgg cgaagtcgga gatacgctcc gacgcttcgg acgaaatacg ggcgaacatc
     4021 gcggaagttt cgggagtgcc actgtcatag ctggcaccgt caaacaggga atcctcatcc
     4081 aggaacaccc cctgctcacg caatgtattg acgaacgcga cgttcagggc cacgcgaccg
     4141 gtgaatcgaa tggtggtcga caagccatca tcctcgatcg tgacgctcac cgggtacaac
     4201 agcaccggca gcgcgttgcc cttccacgtg gccacgccga ccacgagcga caactccgcc
     4261 acgccggaaa tcctgcgttt ggcggcctgg tcatccagca cgcgcccaat atggcgttcg
     4321 gcggccttaa gcacacccgt gtcgcggaac aacgaatcca accggacatg gccggaagcg
     4381 aacagctggg cgatgccgga gggatgggca tgggtcatct ccagcttcgc ggccagttga
     4441 ttcacatcct cgagcggcga tggcggctgc atgctgcggt attggtcacg ccagcgacgg
     4501 atgcgattca gaccttccgt gagattctgg ttctgagtct ggctctgggt catctctcga
     4561 ctcactttcc aaccgcttcg cggtattcct gatagccctt gttgtgagac agggcttcgt
     4621 caatatcggc ctcgccacgg gcggcgagcc aagaatacat gtcgtcgtac aggtacggat
     4681 tcatggcgag gaacgccagc aattcgggat gacgggcgac ttcgaattga acattgaaat
     4741 cttgcgtggt ttttgcctcg tcagaggtca ggccatcgac ttcgactgcc ggctcctgtg
     4801 ctttcagctc gcccttggcc acacgctcga acaccgagcc gggctcgaag accggagtgt
     4861 gtacgcccgt ggagtcgata tcatatgctt cggataccga ctgctgggcg gtttcggcag
     4921 ccggctgctg gtctgctacg cctgctgcgg tggcttcttg atatgcttcg gctgcggtcc
     4981 gctgctgatc gtccggtacg tcagctgccg gctggtcggt ttcagactgg gcggttgccg
     5041 gctgagcggt ctcaggctgg gtgttttcag attgagcggc ttcggcttcc cgggcggctt
     5101 caggctgggc aacttcagca tgctgttcga cagtgtccgc cggctgagaa tcgccggcat
     5161 tcctggtatt cggagcggac tgaacgtttt cggctgactt gcttgcgcca gccaccatcg
     5221 gattcaacaa ccgccgccga cgccactgcc ggctcgcttg tgctgggagc gtgcttaaca
     5281 acggcatggg cgatggcatc tactggaaca ataccggaga tcctgctgtg cttggatgca
     5341 ggcgttacaa tcgtcgactc aggttcggtc tgctgcttcg gcagtgccac ggagttcatg
     5401 gcctgctggt ttttgcgttc ggtctcctgc tcggcatgct gggccaacgc atccgcgaac
     5461 agatcacggg gtatggccgg ctcatcgttt ttcgtcactg gctgcgtgat attcagcgag
     5521 ccaagttcaa gctcgtcgac cttgcgtcga acattatccg cgctgaagat ggcggtcggc
     5581 tcgccggccc gcaaatcaag aatatcgtcg acggacatat ccgccgcatc cggctcctgc
     5641 ggtgcttcgt gaaccgcata gccaaacaaa tcaggcacct tgaggtcgag cggctttgcc
     5701 actggatcgt cgacacgaat gaagtccgca ggcacatcgc cgaactgcat gcgcatggga
     5761 gacgtcggca acaggaactc ggcattggcc ggcaagcgca acaaatcgcc attttcccgc
     5821 accacatacg atccattggt cgatccaaga tcacggaccg aggccgtgcc attgcttttt
     5881 accgtgaaca tggcgtgccg tttggacatc gatttggttt gatcggcgac gtcaaggcgg
     5941 gtgttgccat catcggcaag ggggcgtaac ggcttacggc cgatttccac gcactccccc
     6001 ggcttgacgc tgatccggtc gacaccgttg atcttcaccg tccactcgct caccgtgacc
     6061 gccttcctgg acacactatt tgaccacact acgtgccatt gtgtcatctt ttaccggctt
     6121 ttggcggatt actgggcaca gaattgacaa ctttcgctca ttgccagctt tgtgtttcca
     6181 cgccggatcg cgttctccgc ctcgtcctcg tcaactagtc tactcagtat gcggaaaccc
     6241 cgcagcctga aacacaggtg cggggttcct cgaaaagctg tgattgcggc ttatgccgca
     6301 caaatcactt gagctcgacg ctggcgccag cctcttccag ctgagccttg gccttctcgg
     6361 catcttcctt cttggccttc tcgaggacgg ccttcggagc gccgtcgaca agagccttgg
     6421 cctcggccag gccgaggttg gtgatggcgc ggacagcctt gatgacctgg atcttcttgt
     6481 cgccgacggc ggagaggatg acgtcgaact catccttctc ttcctcagcc ggagcagcag
     6541 cgccagcaac agcggcaaca gcggcaaccg gagcggcagc ctcgacgtcg aacttctcct
     6601 cgaaggcctt cacgaactcg gagagctcga ccagggtcat ctcgccgaag gcttccagca
     6661 gctcatcgtt ggtgtactta gccattatgg cttcctttca atcttggcgg cggacgaagt
     6721 ggtagatccg ctgcctaata aaccttattg tttaaattat cgaagcgact gattcatgcc
     6781 gtgagggcgt aaatcaggca gccttctcct gcttttcgcg cagggcgtcg atcgtgcgca
     6841 cagccttggt aggcagagcg ttgaacaggt acgcggcctt ggccatcgat gccttgatgt
     6901 cgccggcgaa ttcggcaagc agctgcgggc gggacttgag gtctgccagc ttcttggcgc
     6961 cctcggcatc gtagacagtg ccgtcagcgg cggcaccctt gatgacaaga gccttgttgt
     7021 ccttggcgaa gtcacggatg accttcgcag cctcgatgaa gtcgcccttc acgaaggtga
     7081 tggcggtcgg gccggagaga atctcgtcga gaccctcaat gcccgcttcc ttggcggcga
     7141 tgcgtgccag cgtgttcttt gccacggtgt aggaagtatc gcggcctagc ttttcacgca
     7201 gatcggaaat ctgcggaacg gtaagcccgc ggtactcggt caggtagacg gcgtcagcgt
     7261 tacggaattc ttccgtaagc tgagcgacta ccgcttcctt ttcgggcctc ttcatggcgt
     7321 tccttcctaa gtcggccgtt gacttggagt tcggaacttt cggccgggca acaaaaaagc
     7381 cctgcgcaca ggcagagcaa tataatctcg cgaagcgacc ttcgccggcc cttgacgggc
     7441 ttatatctct caacctgcgc tggcttggcg aaccaaactt cggaaatgca ctcgcgcgca
     7501 cttcaaccaa cggtctgtgg tttacggatg attagattac ggatggtcag cgacattgca
     7561 gaattcgggc gtgtcgcggg gcattacagc gtggcggcgt actgggctac ggacgcggtg
     7621 acttccttca gatagtcgag cgagcgacgc cagtggccgg gcacctcgat cgaatggttg
     7681 gcatccgggt attcgtgcac gtgcggggtg agcgcgttgg cgcgggcttg atcgtagaac
     7741 gggtcggcgg tgccggcgca gatcagcggt gccgggccgg agtatcggga gctggcggcg
     7801 tgcgactggc cggcctgttc cgcgcggatg atcgcccttg cttcggccac gctcggggcc
     7861 tcggattcgg cgacgatatc atcgccgaca gccggcaccg gaatcacccg ggcggatgga
     7921 tcgaagtcgg cgtgatgcag caccggggtc agcagcacga acggcagacc gtagtgtgag
     7981 gccacatgtg ggtaggtcat cgtcgtcagc gacttgccga tcagcaacag gcgagggccg
     8041 gacggggtgt cggaatcacg gttcggcgag ccagctgcgc catcggccag cacggtagca
     8101 gaatcatcgg cggcaacgcc atccccggaa tcaccggctt tacccgcgcc ggactcgcgc
     8161 gcggcggcca cccagccgtc aatcgcctga ttgaggaccg gaatcaccgt ggacaggtcg
     8221 tcggaagcgt tgcgtacgtt catgcggtcg attcgccagc cggcctcgac cagagcctgg
     8281 gccgaccagt agagcagcgg acggtcgcag ttgtagccgg tgccgggcat cagcagtacg
     8341 cggccgtact cctgaccggg cttgacgaca ttcttcttcc aagaggtgat gctgacttcg
     8401 acggccggtt tcatcgcgcg ccttccttcc acgggtgtgt cctgcgtgtc ccctgagttg
     8461 ttccgaatta ttctggcagt cagtctactc ccggacccgt tcacgttgcc tccacaagct
     8521 ataatcggcc acggttctga ttgggtgcgc agcgcctgac gcgcgttggg caggccgatc
     8581 agggcgagtc gcaattctcg ccggggccag ccgcagcatt atgcacgcgg tgcgcagccc
     8641 cgcataaacc caaggaagta gagagtaaat ggagaagttc ttccatttga aggaaaacgg
     8701 cacgaccgtc tcgactgaga ttctggccgg cctgaccacg ttcttcgcga tggcttacat
     8761 catcgtcgtc aacccgcaga tcctgtcgca gacgggtatg ccgtggggcg gcgtgttcct
     8821 ggccaccatc atcgcggcca tcatcggcac cctggtcatg ggcctgttcg ccaacgtgcc
     8881 gtacgcccag gccgccggca tgggcctcaa cgcgttcttc acctacaccg tgtgcttcgg
     8941 cctcggcttc agctggcagc agaccatgtg catggtgttc ctgtgcggcc tgatcaacat
     9001 cctcatcacc gtcaccaaga tccgcaagat gatcatcctg gccatcccgg agtccctgca
     9061 acacgccatc ggcggcggca tcggcctgtt cgtggcctac gtgggcatgc tgaacgtcgg
     9121 cctgatcaag ttcactcccg gcgatccgaa ggccgccgcc aagggcggtg ccgttgcggc
     9181 taccccgggt ctggccaact tcaacgacaa ggtgctgtgg gtcttcctga tcggcctcgt
     9241 actggccatt gtgttcaccg tgatgaaggt taagggcggc atgctgctgg ccatcgccat
     9301 caccaccgta atcggcatcc cgttcggcgt gaccacctgg tccaactcgc agtccatcag
     9361 cgagaccttc tcccagctgc cgcagacctt cggcgcgatc ttctccgccg agggcttccc
     9421 ggcactgttc tctgatccga ccaagctgcc gctggtcatc gtgaccatct tcgccttctc
     9481 catgtccgat acgttcgaca ccctgggcac tttcatcggc accggccgcc gcaccggcat
     9541 cttctccgcc gaggatgaga aggctctgga gaacggccac ggcttctcct ccaagatgga
     9601 caaggccctg ttcgccgact ccatcgccac ctccatcggc gccatctgcg gcacctcgaa
     9661 caccaccacc tacgtcgagt cttccgccgg catcgccgca ggcggccgaa ccggcctgac
     9721 ctccgtggtt gtggcgatct gcttcgcctt gtccgcgttc ctggccccgg tcgtctccgc
     9781 cgtgccgtcc gccgcaaccg ccggcgtgct ggtgatcgtc ggctgcatga tggccgcttc
     9841 cctgaaggaa gtcaagtggg acgacatcgc cgaagccatt ccggcgttct tcgccgccgt
     9901 gttcatggcc ttctcctact ccatctccta cggcatcgcc ggaggcttca tcatgtactg
     9961 catcgtgaag acctgcaagg gcaaggccaa ggaggttcac ccgatcatct ggactgtggc
    10021 cgcgctgttc attctggact tcgtgtgcat ggcgattctg taacgctgat tgacaacgga
    10081 ttagccttta aggattaatc cgccgccata cggcataaag gccgggctct cacaagggcc
    10141 cggcctttgt tgttgccaca gtgatcaacg cccatgagta ccttcgatga tcatccgcca
    10201 ttcttgatgc atgggaatag ttgtcaaccg acaactaaac gtacatggcg gcagcacgag
    10261 tgccttattc aacatcgaaa tatgcagcgc gaagaagccg aaaaaccaaa aaggcttcta
    10321 taccgaatga aacggtatag aagccttttg gtttgcgatc aagcccttct cacccgagag
    10381 aagctagaga acgatcagtc ctcgaatgcc gaaccgttaa gcgggaagcc ctacgggctt
    10441 cccgtaggtg aggggagcgg ctatgccgcg accgatccgt gagagggatt tagtcctcga
    10501 acggatcaaa gtcgcgacgg acgcggcgac gcgggcgctc agtacgctct tcgcggtcgg
    10561 cgcggtagtc gtcgtagttg tcgtcggtgg cgtagcgcgg gttgcggtcc gagccacggc
    10621 cacggccacg ggaaccgcca cggcggtcat cacgatcgcc acggtcatcg cgatcggaag
    10681 agcgacggcg acgcggacga tcatcgtcac gggagtcgcg agcatcacgg tcatcgcgat
    10741 cgtcgaagtc acggtcggca gaacgacggc gacgcggacg atcatcatcg cgatcatcgc
    10801 gatcgaagtc gcggtcatcg gagcgacggc gacgcggacg gtcgtcgtaa tcatcctcga
    10861 agtcatcgga gcgacggcga cgcggacggt cgtcacgatc gtcgaagtca cggtcatcgc
    10921 gctcggagcg acgg
//
LOCUS       DAWBMW010000042        10828 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_1532_length_10828_cov_3.034531, whole genome shotgun sequence.
ACCESSION   DAWBMW010000042 DAWBMW010000000
VERSION     DAWBMW010000042.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 10828)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10828)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10828
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1532_length_10828_cov_3.034531"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4958..4967
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6519..6528
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1955 a         3236 c         3452 g         2165 t
ORIGIN      
        1 ccgaatcagc cgaatcagcc gaatcagccg gtgccgcatc tgtctcattg tgatcgtcgg
       61 cagccgactc gtttccgctc aactcacccg gctggtcatc ggcctcgttg tgtacaggct
      121 tgacttccgg gacgggcgca accaccggca atgccgccgc ggcagcggct tcctgatcct
      181 ggccagaggc cgccgcatta ccgccgaaag cgaatccgga gaacgcctgc gagaacatag
      241 aacgcagttc cgaaacacgc tgggtgatga cggactcgcg ttcctgcaga tcggcgatgc
      301 gcttggacag accgtcaagc tcgctttggg cggcggcgcg acgctcctcg atctgcgcat
      361 cggcttcctc gcccgcctta tgtacgattg cggcggcctg gatgtccgcc tcctcgcgct
      421 tggacgcggc atacgaatcg gcttggtcgc gagtgttctt ggcctgaccg accaactcat
      481 cggcttcccg cttggccgcc acacgacgct cttcgaggtg ctgcagcagt tcgttgacct
      541 tcttcgtggc ctcatcctgc tgagcggcga tgtcgtgacg gatctgctcg acctcggcgt
      601 tgacctggct catcgtgctg gtgcgcttga cctcggcctc atcagtgatt tcctgaccct
      661 tggttttggc cgcatcaatg atttcgctgg ccttgcgctg ggcttcggtc atcatcttgg
      721 acacttgctc gcgcacgtcg gcgagcttct tgttggcctc ggcgagcgcc gcctcaatct
      781 gatcgttgga atcagactta agcttggtga tctcctcatt ggcgcttttg cgcattgcgg
      841 caacctgctg ggtcgcctcc gccttcatgt cggcgatttc gcgctcttga gtggagcgtt
      901 cggaagccat cttcttggtg tgctcttcgc gcgcgttgct gagctcaagc tcgacggtct
      961 gacgctgttc ggtgatggtc ttggcagttt cggagcgcag ttgtgcggca tcctcgttag
     1021 cggaggtggt cactgattcg gcctgattgc tggcgttcgt cagaatcgag gcggccttgg
     1081 cattggcgtc gtcaacaata tgctgggcat ccagcttggc gttgttgatc agtgtttcgg
     1141 cctgagcctg agccgtggta cggatggagg acgcgtcctg cttggcgcgt tccagcagtt
     1201 cggtactcgt ctgttcggcg gaggcaagca tctgctgggc gttcgctccc aaggaagcga
     1261 agctgttgcc ttccggcttc ttgttcttct cttcctgaag ttgggcctgc aattgcagaa
     1321 tacggtcgtc atccgccgca atctgctcac gcatgctggc caacgtctgc tgcgcacgct
     1381 tcatactctc ctgagtagcg tcgaaggcag catcaacctt ctccttgtca tatcctcgca
     1441 gcaccaccgg gaaacgatcc accatcgctg tttattcctt tcgcacacgc ataaagctct
     1501 tggcacatcc ttgaagcact gtagcgcatc gcactagcgc tgagcgttga gcgacgcgca
     1561 atcgttacaa tgcgtgcgaa ccatgttcta ttccggcgtg gcgcacagcg tgctaggcga
     1621 tgaggatgtc ggagctggag ggcttgagat agtagcgcac ggcttcgcgc accacatcac
     1681 ggccagcggc gatctgctgg tcgagtggta gaccatgctg gctggaatgc gccggaacga
     1741 ccggagggaa gctgacgaat ccgcccagca cccgctcctg ggtggcggtc cagttgagca
     1801 agttgtagaa cagcgaatta catacgaacg tacctgcgtc cgaactcaag gtggccggaa
     1861 tcgaatcgtc cgtgaaatca ttcaggatcg agcgcaacgg caggcgggtc caataggcgg
     1921 ccgggccatt ggggtcgatg ggttcgcggc gtgggatcac gttgtccgcg tctggcttga
     1981 tcgcgtccat caggttggtg gcgcatcgtt caagcatcac gcctcgggct gcgtgcttga
     2041 gcccggtggc gatgacgata ttgggcttgg tggcctcaat cgtctctttc agcgtgggcc
     2101 aggctttggc gaagctcacc gggatgctga cggcatgaac ggtgacggcg acctgctcca
     2161 acggatcgtc cggagcagag gagacgccca gtccctgctc ggcgatggcc ttgggcactt
     2221 cgacggcggg attgacctcc acgccgtcat agtggtcgaa gccggagatc acaacattga
     2281 gttcacgcat aatgccagtc tagcgatgca tctggccggc cgcttgggcc agcagggcgt
     2341 gcatgcgggc gcgggcctct tccttgggat cacgaccgag aatctcctcg gcgatacgct
     2401 cagctgtggg aatatcttcc agcgtggtga tcttcaggtt ggtttccgca ccggagacaa
     2461 tggcgacggg ttcatcaggc agataatcga ccactacgcg cgtgtcgtcg gtgggatgga
     2521 agtccgggtc ggcggcagcc aaatcgtaag cgtgacgaat cgtcgcgaag cggaaggatt
     2581 gcggggtctg cgcgcggaag gtgttcgggc ggtccggcac cgacttgacc actttcaaat
     2641 cgcccagatc ctcggtcaac agcacggtgt cggttgaggc gtaggccacg gtcgcggccg
     2701 tgaactggtc gagcgcatca atcgaaccgt cgatggcgga ctgttccacg aacggacgga
     2761 cggcgtcatg gatcagaatc ttggcatcgt ccggaatgcc agccgtagcc agcatgtcga
     2821 gcgcggtggc ggtactgtcc acacgctcgt caccgccgtc aatgatgacg cggaccttgg
     2881 tgtagcccat ctcgccaacc aacgtttcca cttcaccgcg caccttggga ttgaccacca
     2941 ccacgatgtc gctgacgcgg tcgcaatgtt caaaggcatc aatgctccag cacacgatgg
     3001 gcttgccgcc cacggagaca agctgcttgg gattatcggg gtcgaagcgc gtaccgaagc
     3061 cggcggccag caccacggcc accaccggca cggtttgcgc cggtttggcg ccctgagcgg
     3121 gggccggctg gacggtaggc gtctcgacgg gggtatcgaa atctctctca gtcataggca
     3181 ccaccgtagg gtgagggctg gccgtccgtg aactgcatca catcttctcc acggaatgtg
     3241 ctcgcattct ccgtttagtc acgcacgatg agggcgagga ggcggccttc gtgtgttttg
     3301 ttggcgagcg cggcgcggcg gtgcgagtac cacagtgggt tttcgagcgt gcacatgctg
     3361 tggctgatgt tgtcgatgcg ctccgcgagc tcggccgggc cttcgccgtc cgtacggcac
     3421 agttcggcga gttccgggtc ttcctcaagg tactgggtgg cggcgtgcac gcgcggcatc
     3481 gaatcgacca cctgatgtac accggcgaaa gcgaggtcga tcatcgcggc ttcggcgatg
     3541 tcgatgccgg caccgccgaa acgggtcttc gtcttggtca gcgggaagcg cttgatgaac
     3601 tggtcggcga tctcgtcgcc cacttcatag cagtcgccgc agatgcgcgg gccgagtgtg
     3661 gccacgatgt tcgccgggtc ggcacccttg ctcttcatca ggtccaccgt ggtgccgatc
     3721 acgccgcgct ccagaccacg acggccgcag tgggccgcac cgatgatacc ggtgaccggg
     3781 tcgcccagca gcaccggcag acagtcggcc gcgaacatgc ctagggcgat gccggattgt
     3841 gcggtgacct ggccgtccgc ctcgatgacg tgcggagtgt cagtctcacc atgtgtgccg
     3901 gacacgtcga agccgaaggg ggtgttgatg acgaacgagt cgtccacgtc cacagccacg
     3961 ccggaatgta cttgggagac cagcgatagg cgggcttgca ccgcttcggc gagtgcgatg
     4021 cggttcgaca gcacggcctc aggttcgtcg ccggacttgc cgccgaggtt caggttgccg
     4081 taatcgccgg tgctcaggcc gcccagtcgc gtggtgtaca ccaccttgat gccgggggcc
     4141 agtgcgatgg ggatggtcac cggaataggg cgaccggctt tatcggtaga gctgatgctg
     4201 ttggaatcga ggatttcttc gctgtattca ggcataaggc attctttctt gggtgcgggc
     4261 ggtgtccatg attttcatga ccacccccag tcagcggggg cggagtggta ggttattcgg
     4321 ctttgagggt gggctttttg gtgcgcgagc ggatggcggc gttgatgggc acctggtgcg
     4381 ggcagggcat cgagaacacg tgcgccggat tattgatggc gggtactggc gcgccatcgg
     4441 catcgcgcag gccggtttcc ggcacggtaa agaccaatgg tctctcgcca ggcagcagga
     4501 attcgacctg ttgaccgggc tcaatcttgt tacggctcat cagcgtgaca cgtccgtctt
     4561 cgtccgcgct ccagctcagc acttcgccca ctaccagcca tgagcggaag taggcggagc
     4621 ggtcggtgtt ctgtgtgacc ttgtgctccg ggtaatagaa gccggtggag tagtcgcggt
     4681 gggccacctt gtcggtttcc tcgagcagcc attcgggcag ttcgacgtgt ggggccgggc
     4741 gaacggccgc atgtttccaa tcggtctcaa tctgttcgac cgcggtgttg gacttgcggc
     4801 gcgtggaacg ggcgtgagag ctcatgccgt cgggttcgac ggcggtgccg gccggcgtac
     4861 cagaggcagt tgagtcgacg gcatcagcaa ccgtcacgcg gtcacgttct gcggcaaccg
     4921 agccggtgac cgaatcggca accgcagtat cagagccnnn nnnnnnncgg ccacaccggc
     4981 gaacgcggca tcagcattgc gcatcacttg atcttcggtt ccttggccaa aatcggggtc
     5041 gcccgggcga atcacacggt catggaaagg cttcaaaaca ttgccatcag cgtcttcaaa
     5101 accgcgttga atcatgtatt cattaaccgc ggttttgtag gcgttggtca tggcggcgat
     5161 atagtaggcg cttttcgcgc ggccttcgat tttgaggctg gtggcaccgg agtcaatcaa
     5221 atcatccaag tgccccagca tattcatgtc ctgcgaattg aacagatagg cgccgttctc
     5281 ggtctgctcg atcgggaagt actggccggg gcgcttctct tccacgatcg aatacttcca
     5341 gcggcatggc tgggcgcatt cgccgtgatt gccatcgcgg ccggtcagat agttggagaa
     5401 caggcagcgg ccggagaagg ccatgcacat agcaccgtgg acgaagcatt cgatgtcgag
     5461 gtcatcggga atgttggcgc gaatatcacg cacggcctgc aagtccatct cgcgggccaa
     5521 caccacacgg cgagcaccaa gctcgtagaa ggcgttcgca gcctgataat tagtcacgcc
     5581 ggcctgggtg gagacgtgca gttccagatt cggagccacc tgacgggcca tcatcatcac
     5641 gccaatatcg gagacgatca gcgcgtccac gccggtgtct ttgagctggc cgacgtactc
     5701 gcgcatggct tcgatctcgt tgttgcgcgg cagcacgttg caggtcacgt acacgcgagc
     5761 gccgcgttca tgggcgtagc gcgatccttc ctcgaaatcc tcaaggctaa ggtttttggg
     5821 ggccgatcgc atgccgaagg ctttgccgcc gcagtagacg gcgtcggcac cataatcgac
     5881 cgcggttttc aggcccctca ggttgccggc cggggacaac acttcgggct ttttgcgggt
     5941 ggggaggttc atcgtcgtat tcatcaccgc aatagtgtag tggtggccgt gtaatgcggg
     6001 aggggctacc gttaggctgg atgacatgaa gatcgatatc gtgagcgtgt tccccgaata
     6061 ctttgaggtg atgaacctca gcctgatggg caaagcccag gccaagggat tgttggagat
     6121 caaggcgcac aacctgcgcg actggaccca cgatgtgcat cattcggtgg atgatacgcc
     6181 agtcggcggc ggggccggca tggtgatgaa gcccgaagtg tggagcgaat gtctggacga
     6241 actgctggga ttctcgcagc cgtccggctc ccccgcccca tccggcgcac cggtgctcat
     6301 cttcccgaac ccatcggccc cgttgttcac ccagcgcgac gccaccgagc tcagccatgc
     6361 cgaccatctg ctgttcggct gcggccggta tgagggctac gatgcgcgca tcccggacta
     6421 ctaccgcgcc caaggcgtcg acgtacgcga atattcgatc ggcgactatg tgctgaacgg
     6481 cggcgaagtg gcggtatcgg tgatgcttga ggccatcann nnnnnnnntg ggcaacccag
     6541 attcgattgt tgaggaatcc tacaccggcg agggcgcact gcttgaacat cgccagtaca
     6601 ccaagccggc cgtgtggcgc ggtattgcgg tgccggatgt gctgttgtcc ggcgaccacg
     6661 gcaaggtgga ccgttttcgc cgcgacgaag cgctggcccg caccgccgag attcggccag
     6721 accttatcgc ggcactggat tgcaaggcgc tggataaggc cgatcgcaag accctgatgg
     6781 ccttgggctg ggaggtctcc gccgcccatc cgcgccggct cgccgattaa ttcaccacac
     6841 ggtgggagat tcactacgcg gcggaatcgt cggaacgctc tatatccgag tcgtcatccg
     6901 cggattcgat gtagaagacg gcggtttcgc cgtagttgcg gctttcggtg atttgccagc
     6961 ctccgggggc agtgggatca tccgagcgta ccgagcgttc gagcatgatc accgtgttct
     7021 cgttggtggc gctgcccgcc gccagatcgg acagtagctg attgcattca gcggtctcat
     7081 aggcataggg cgggtcgatg aatatcacgt cgaatggttc gccgaagccg ccagccactt
     7141 gctcggcttt tttgaccagc acacgggcct tgagcgaagc atcccacgaa cgattcttct
     7201 gtagctgcgc caaggtcttg gtaatcagcg ccgccgcagg ccgggaactt tccacggcca
     7261 cgagttctcg cgcgccacgg ctcagcgctt cgatgcccaa cgcaccagta ccggcgaaca
     7321 ggtccagtac acgggcatca tccagcactc cccatgagtc gaggtgcgag aaaatcgcct
     7381 ctttggtgcg atccgtggta ggccgggtac ccgttttcgg cgtagcaagc gccacaccct
     7441 tgaatcgtcc agaaattaca cgcatagctt tcactgtaca ggttcatctg cgcataccaa
     7501 tcatgaacaa caatggggtg ctcgaagcgc accaatgata tatgcacttc gagtacctca
     7561 tgtacgaatg gtcttgcctc cactgaagca cattcgaggg cttcccgacc gtgatttcct
     7621 cacgatcggc atacggaaaa actatatcgt gcgattacgg gggcgcagcc ctagcctcag
     7681 ctggaggtgc ctcagctgga ggtcaggaag gtttcgttgc ctcgggtgaa gtcgaggacg
     7741 gcaccggcga gttggacctc acctgccagt tcggggtcgg cggccagaag ctgcccggca
     7801 cgggtgcggg cgtcggcgat catgtcggcg tccttgacca cgcgcaggag cttcaggctg
     7861 gacttgccgc cggattgggc gtcgccgagc acatcgccgg caccgcggaa ttccaagtcg
     7921 gcttgggcga tttcggcacc gtcgagcgag tggtgaatca cttcgagccg ttgctcggcc
     7981 ggggagccgg gttccgcacg ggaaataagg aacgcccacg agttggtgcc gccacgcccc
     8041 acgcggccgc gcagctgatg cagctgagag aggccgtatc ggtcggcatc aaagatgacg
     8101 atgcagctgg cctgcttgac gtccacgccg acttcgatca ccgtggtgga caccaggatc
     8161 ggagtctcgc cgccggcaaa atcggccatg acctgggttt tgacgtcatc cttgtcgcgg
     8221 ccggtcagag tcgcaaaccg aatgccttgg aactgtggaa gtttctgcaa ccggtcggcg
     8281 atttcggcca ccgcatgcaa tggcggggga gccacggttt cgccattttc gtcgaaggtt
     8341 tcgtaggggt cgtcgatgcc gatggcatcg gccgccgcgc gcgtcgcacg gccgcccgca
     8401 gctgtgcggg cactggaacc agatgctgcg gcgcgtccgc gggcagaacc ggctgctgcc
     8461 gaaacgccgg aaccgccttc gttctcctca tcatccgcat caatgcgcgg gcacacgata
     8521 taggcccgct cccccgcgtc gactcgcgcg cgaatgtgcg cgaacatacg ccccatggtg
     8581 gcggcgtcgg cctcattgac caccacggta cgaatcggtt tgcggccgcc aggcaattcg
     8641 gtgagccagg aaatatcgag atcgccaaac caagtcatgg cggcggtgcg cgggatgggc
     8701 gtggcggtca tcaccaatag atgcggcgtg gtgccgtcat cggttttggc gttgagactt
     8761 tcgcgctgtt ccacaccgaa ccggtgctgc tcgtcgatta ccaccaaggc caagtgcggg
     8821 gcttggaagg tcttggaaaa cgccgcgtgc gtggccacga taatgcccgg ctcaccactg
     8881 gcggccgcag cgagcgcctt acgccgggcg gcaagcttca taccaccggt cagcagggtg
     8941 acgggaattt ccgcagtcac cttggaagaa gagaccgtcg aagcaccgga cgcgcccatc
     9001 gcgccctcaa cgtcggccgc ggcatcggtc tccttggcac ccggctgtgc aagggtcaac
     9061 cccgacacca tcttgctgat ggtctcgtaa tgctgttcgg caagcacctg agtgggagcc
     9121 accagcaccg cctgatagcc ggcgccgacc gcctgcagca tggccgcgag cgccactacg
     9181 gttttgccgg agccgacttc gccctgcaac agacgctgca tgggccagtc acgttcaaga
     9241 tcggacgcga tgtcgtccac cacctgactc tgcccggctg tcagtgtgaa cggcaacgat
     9301 gcgatgaagc ggtcgcgcaa attgggcaaa ttgggcagat caggcagatc ggtcgcagaa
     9361 ccgcgctccg atgcttctgg ttgcgtactt ggctccgcaa ccgcttcgcc caccgacgct
     9421 cgagctgttt ccagcgcctc gttcagcggg caaggatgcg acgatgactt gtgcgcgtgc
     9481 tgtcgagact tcagcagcga ggtctgcgaa acgaaagcct cctcatagcg cagggtttcg
     9541 atggcctcct tgaaccgggc cgtggaggcc ggatcatgaa tggccaggaa cgcctcagcc
     9601 cgatgcatga ggttcttggc cttgcgcacc gattcgggca gcacatctgg gatggattgg
     9661 gagagcgcct cagcaccgga ttcggcggtc gtgccggact tctcttcccc gttttggacg
     9721 gcgatggtgt cagtcgcggg agccacaatg ccggctgccc cagcgccagc gagttgcccg
     9781 tcaggcgtgg atgacgtgcg ggcccccatc atccacagca atcccagaat cgtctcatga
     9841 atatgttcgg acgagatacg cgaactggcg tgatataccg ggcggggccg gcagacgcgg
     9901 gtaagcgcct cttgcacggt atcggcgtcg tatttcaacg cggccccgga aggggcagtc
     9961 gcggccggcg gatacgcgga ctgcgcactg gcatacggat cggcacttcc cgcaaacgca
    10021 ccattgccgg atgccgcgcc ccgagcgtaa ccctcaagcc ccgctcctgc tccggccggc
    10081 acgggcgcga cagtgaggat ttcgggatgg gtgaactgca gctgacccat gtattcgccg
    10141 ggcataccgg agaccaccac agacgtgccg gcgcgcagtc gcacgctgac ccaatccaca
    10201 tacgatttac gatacgagaa gaacgtgagc cgggccgtac tgccgggtac gcgacgagac
    10261 cgggcgaagt cggcgtcatc gaccgtcgct tcgagtcggt agccgcggcg agcgttcatc
    10321 ggcaccacgc gcatatctcg tatcacggcg gcaaatgcca tctgctggcc gggtgcggcc
    10381 tcgcggatgg cgcgaagcgg caccggttcg gtgacgcgga aaggatagta ggtcagggcg
    10441 tcgccgacag tgacgattcc cagcgatttg agcgcggaca cgcgacgctt gttggtgacc
    10501 agcgaagcga gagtggtgcc catagtgatg ctcatattgg cctattctat tcgcacggcc
    10561 tctttaggcc gcagaaacaa caaaacccgc caagccgaag ctcaaacggg ttttgctcca
    10621 cgttagtacg cgattcacgc ggccacgcgc tgaaccttgc cagccttgag gcacttgacg
    10681 cacacgcgca ggcgagtgtt ctcgccatca atcgtggtgc gcacagactg caggttcggg
    10741 cggaagctac gcttggtacg acggtgcgaa tgggaaacgg tgaaaccggt ctgcggtccc
    10801 ttgccgcaca ctgcgcaacg agctgcca
//
LOCUS       DAWBMW010000043        10350 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_1596_length_10350_cov_2.452841, whole genome shotgun sequence.
ACCESSION   DAWBMW010000043 DAWBMW010000000
VERSION     DAWBMW010000043.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 10350)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10350)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10350
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1596_length_10350_cov_2.452841"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4615..4624
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9491..9500
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2319 a         2968 c         2748 g         2295 t
ORIGIN      
        1 cacataatcg gccagtcgcg ggcgcatcac cgcatactcc cagccgccga tggcgcgcga
       61 cgatttccac ggcttgttct tgtctttctc ggggtagacc tgattgacct gtgcctcgcg
      121 cttggcatgc acggtgagga ttacggtgcc ttcggtcttg ccaatcacat cgtcatgcag
      181 aatcaggcca tgctcggtct gcgtggagcc gccggccaca agctgatcgg tgatcttctt
      241 gcccttgcgg tcggtgaact gcactttctc gcctgcggtg agaggcccgc gcctcggctc
      301 cataacttgc tccttgcgtg ctcggtgggt caatcgctac aagtctaatg catgtaagct
      361 cgctggtgag gtgatgacga tgcgaatgca gatgacaagc cagatcgatg ccggcggcaa
      421 tgaccccatt ttcctgatgt tccacggtta cggcaacgac gaaagcgaga tgatgcgtat
      481 atgcgccgac gcgagccgat gcggccccgg ccgcaattgc cgccgtaccc agctatatct
      541 cgttccgcgc cacctacgcg aggccctata tgggcggcaa ttactggtat cccgacggct
      601 gcggcgtgga cgagcgccaa cgcgaatgcg cggcggtcgg ggatgcggtg gcttcactgc
      661 tggatgcctc ggcattcgcc catcgcagga agatattgat cggtttctcc caaggcggtt
      721 atctgtcgta tcgtctggtc aaggtgcatc cgacgttgtt cgatgccgcg atcctgctca
      781 gtccttcgtt catgggcgag gaacaggcgg cgcttgactc ccctacccga ttcttcctgg
      841 cgtatggcgc gcaggaccgc atgattccgc tgcccgacca gcaaacggcc catcaggtcc
      901 ttgaacgggc cggacatctg acctatcgcg agtattccgg catggcccat gccatctgtg
      961 atgaggaaat cgccgatatc cgggatttcc tacgtaccat atgatgccgg aatagcttca
     1021 atatggaata tctccgttca aagaggtacc ggtccaagta gaagcggctc aacaatggcg
     1081 gaatggcgtt ggccgcgccc attcatcgcg cgatggttct gcgatcagct caattcccct
     1141 gccggctgat gccgccctta tgaccggtgg ccttgaatgg cgtggcgcgt gccgtcacct
     1201 tcggccccgg cagattcgta ctggtattgg atcgaccgcg gagcccggca gccggctggg
     1261 actcggggcg tcgaccagta cccgtgatgt cggtatgccg tttgccgtcg tgtgcctgtt
     1321 tcccaccagc accgtcagcc tcgtcaatat caatgccctt ggcacgcaaa gcacgcgccc
     1381 accgctcatc catcgtgcgc ctcacacgtc ccattcgggt ttccacgtgt actgcatgtc
     1441 gtaggcgctg ggcgaacggc ggcaggtggt tcaaggtgat cgggccgaat gcggcggcgt
     1501 caaaagacat cgtttgctcc cttcgatggt gttggccaac aggttccaag ggtcaggcac
     1561 agcgcctttc tcaacccgtt cgccgtcacg atggctggca gcttgcgttc actggttccc
     1621 ctgcaatgcg cctcattatg gcaacacgaa ccgacaggtg ccggctgcgc ctaggatggg
     1681 aaacgattgc tgcacgccga aaagggaagg cgactgtcat gaggtatttc acgactggtg
     1741 gttggaagac cgccgcgcac ttaacctgtt cgaaagtttg gagccgcacg atacggttga
     1801 gattgaggga ctcggtggtg cacgcccgga ggcactgaat gttggcttgg atgcttggga
     1861 tttgatgccg atgagtgaaa cacgggttgt cgagtggttc cacaatcacg atgtcggctc
     1921 gcccgcgaac gggaccagta aggccgatga ttcagttccc tcgctcgata tgccgtcgtt
     1981 ctgatgtgat tcgcggaatg ttcgtgtcgt tgttctcgtc gtgggtggat tgacacagca
     2041 ttgactgccg ttgagatgta tgcacgctga acacgcgcct caacggcagt ggtctcgtca
     2101 tctctcgctc acaccaacgg attgccggct gcctgataca gcgaccacca gtcacggcgt
     2161 tcgagttcga tatcggcacc ggccgccatc tggcggatgc gctccggtgt cattgagccg
     2221 agcaacggct ggataccggc gggatgacgc agaatccaag cgacggcaat gccgttgggc
     2281 gtggaaccgt atttgccggc aagacgggtc aattcggcgt tcagctcggg gaaatgctcg
     2341 ttgccgacga acggaccgta ttccgtgccg gattggaatg ggcaccatgc ctgaatggtc
     2401 atatgcgtca gccgtgaata aggcaggatg ccaccggtgc gatcggctgc gggatcgctc
     2461 gccatattga attggatttc agcgtcgagc atctgtgtat gcatcagacc gaactgcagt
     2521 tggttgattt ccaatttctg acgaaggccg ctttgcaata gttcgatctg ccacgggccc
     2581 atattgctga ctccgaaatg acgcactttg ccgcttgatt ccaacgcgtc gaatgcttca
     2641 gcgacctcgt tcaactcaaa cagcgtgtca atacggtgca gcagcacggc gtcgacgtaa
     2701 tccgtctgca aagcctcaag ctcgtgatcg agtgcgtcaa tcagatgctt ggcgctgaag
     2761 tcatatccgt tggcgccgcc atcgttttcg ccgaaactgt agtcgatgcc gaacttggtt
     2821 tggatgaaga tggtgctgcg gtcgagtcct gcgtccttcc acgcttggcc gaaacgcttc
     2881 gaactggcgt gcgcggcatg atcatggaat ccatagatgt ccgccgtgtc gaaatagtcg
     2941 atgccagaat catgggccac ggccacggta cgtgccgcat cggcggcatc cagcgcgtcc
     3001 atgcgcatca cacccagcgc cactcgtgac gcaatcatgc cggattgtcc gatttcctga
     3061 gtcttcatag tgtctcattt ctgttatcgt gaatcggcga acgggcactg tccgcctcgt
     3121 tgccgggcag caatctccat tgccgcggct tatgtcacct tatcgcttga agtgcacttg
     3181 aaggcaactc gcaggcaggc agcgcgcgct gccgggcgcg tcttcgcgtc ttcatgtctt
     3241 cgctgtgatc gagacatcgt tgtgccgtga tcatgcctat ttcttgtatg atgacctcta
     3301 tagcaacaga gctaggggga ttccatgaac actaccgatt catccaaagt cagtgatgcc
     3361 aacgccggcg caaacaatgt catccatatc ccgcacaatc ccacgcttgc cggattggcc
     3421 acgctgaccc gcaacgtggc gtacaagacc ggggccgatg atctggtgat ggatatcatc
     3481 gccccgcaat ccaccggcga tgacgataac cgccgctacc ccaccgtggt attcgtgcaa
     3541 ggcagcgcct ggaccacgcc gcatcgcgac tacgagatcc cgcagctctc ggctttggcc
     3601 cgcgaaggct ttgtggtggc tacggtgaat catcgcgatg cctcgagcga tccccacgac
     3661 gtgttccccg catatctcga ggacgtaaaa gcagccatcc gctatctcag ggcaaacgca
     3721 cgtcaatggc acgtggaccc cgaccgtctc ggtatctggg gcacgtcgtc cggcggcaac
     3781 acgtcgttgc tggtggggtt gaccgccgac gatccgcggt atgaggacgg caccaatgcc
     3841 gatgagagcg atgcggtgaa gtacgtggtc agctgcttcc cgcccaccga catgcttgaa
     3901 gctgtcgacg cattcgacga tgagaccaat ccattccggc tgtactattt cggcccgttc
     3961 gcggccgtgg tgggtgcgac ccatgagacc ggcatcaatg ccgaggtccg tcagcgcgcg
     4021 gctgacatga gcccctacct gcaggtgcgg gatgaccagc agtatccacc gatgctcctg
     4081 ttgcacggca ccgccgacac cgtggtcccc taccatcaat ccgtcaagat gcgcgaccgg
     4141 ctggccgatc acggcgtgga cgcgcagctg gtgctagtcg acggcgccga acatgagtac
     4201 gatttctgga gccagcaggt cttcgacgtg attttcgatt tcattcgtga gcggagctga
     4261 acggtaaggg cacacgggcg cgtccttttt ataggttgcc aagttctctt ttagccaatt
     4321 ttgttggagc ccttatctgc gcgaacatac catgacagtt ctagactgtg ttctaggact
     4381 gtcaatcggc aatgtgtatg aagcgtgatg tgatgggtca gacggcgatt gatggagttg
     4441 agggacttca tagggccggt gaaatcagca gattcagcgg ttcgcggtcg gtggcgttgc
     4501 cgcctatacc attctggccg tgcttacttc gcgactgttg caacggccga ctaccacgga
     4561 actgttgctc atcgtcatgt ggatcacgct ggaactatgt gcgctgacta tgttnnnnnn
     4621 nnnnacccat gcccgctttc atcgacggca ccacaccact gattgccgta acggtgtttt
     4681 ctgagattgt cgtggcgatg attgtgtagg tccggggatt ttgtttgcta tttcgtgtta
     4741 ttcaattgtg tcccgaatat atatgggcac tgccaaattt atgattccaa tcttttcctg
     4801 cggatcggct taagataatt gactctgaag gatttccaat caccaaaaac ttgttgtccg
     4861 tttcagtttg ttccccattc cggaatttct cacaggtcaa ttcaagggtg catagaaaat
     4921 ccgcaacttg ttcaagccgg tagtcgcgtg gagaagcgtc acgatagaca atggcatcta
     4981 tggagagtgc atactcaatc ccgcctcgca gggcatcagt aactatctgt tggccaccgt
     5041 cgtaatacac cttgacagtg ccataagaat ggaaatccga aagatgcgcc aaaagtagat
     5101 caacaatatc gcgtttgagt tgcgcttcaa aacgttggcg gttatcgtca aaatcacttt
     5161 tacgatgcac aaacgtccga tatgtaatcg gcagccgccg agtcatgata acgaataaac
     5221 ccagaagcct cttacgctcc gataggctca tcccttcgta agcatcatgc ccggtaagca
     5281 gcggcccagc atggaaaggg acatcaggaa gtccatagtc agccaacgac tggcggtacc
     5341 gctctaaggc aagactaata tccttagact ggtcatgaaa aacaagagta attaaataat
     5401 attctgatcg tttaccccac tcaccgctct catcagcgaa gatgctcaat acagacggca
     5461 tcgcaccccc cgtccccgtt gacaaaaaat gccgggggag ccccccggcg acttggagcc
     5521 tttctcgttt ccgagtaaag caatatattt atagcatttt cagcccctag cccgcaagcc
     5581 acaaacggct attccgctga cagctgctca tattgtagcc acgccatcta ctgcaacatc
     5641 atgtcacggt gcttgtgcca acggaacaga cgctcacgat ggcggttcag cgtgaaacga
     5701 tcccacatct ggccctcggc ccactcaacc atgccatcat gttcttcatc ttcgggattg
     5761 cccaaggtat caaggaaccg ctcgaaccca tacaccccgc cgacatcctc cggaggcgca
     5821 tcccccgccc catcccaaag ctgaggatcc gcaacgtgca cgctgcggta ggtacgcagc
     5881 agcttaatat catggatcca atcgtcacca taatcgtagt aatagcgagc ggtacgcgcc
     5941 ttgggaaaca cgtcgcccag ctcggtttcc gaagcaatgc gaacttccgg caacggctcc
     6001 accggcatat attgttcgta tttcgcctca tggtatgagg gctcttctcc ccacaattct
     6061 tccacttgca cctgcttccc atccggaaat gtggcgcaga agcagaacag atgctcgccg
     6121 taccacataa agcattcctg caataccgca tgcaaatcga agaacgtcaa tgatgccggc
     6181 accagaatct cccgccagca ttcacgcccg tcaaggcgta aagtgactcg gaaacgaaat
     6241 gccgtgttgc cattgcctgc catgaagact cccttatcaa cgatgaagtt attatcaccg
     6301 taacagagtc tggggcaaac cactgcgtta cgaatctgat tcgcgccctc agaaaaggcc
     6361 gaatcatctg ttcaatctcc gtgtcctgtg gaaaagactc taacaataac cggtactgtt
     6421 catcatcagc aagtccaata tgccatgtga ccaggtgcgg gacactcatg tgatcagtcg
     6481 ctatggtggg gagacgtgac tttggttcgt actgtcgagc agacgcgaat ggggatagcc
     6541 gcgctgcacg tgtccgatat cggctggggc gtgctgccgc tgtactggcg cacgctgtcc
     6601 agtatgaatg ccatcagcgt gctcgcgtat cgtttggtcg ccacgttggc ggccatggtc
     6661 gcgctgttgg tggcgttctc cggcctgccc accgcgattc cgctggccac gttctcctac
     6721 ggcgtgcagc acagccatta cctgaccgtg agtttcatcc aatatctcaa cccgctgatc
     6781 cagttctgtg tggccgtgct cctgctgcac gagccgatgc gcgcgcaggg ctatgcggcg
     6841 ttcatggtga tttgggtcgc gatcgcggtg tattcgttcg gcgcgattcg ggcgtattgg
     6901 gagcggctga agccctatgc tcgttaagcg cttcctcaac tcccctcccc gaggggagct
     6961 gtcgccgtag gcgactgagg ggaggtcgac acggcaaaca gcagaaacct cgctcccgac
     7021 caaagccaca agcaccactc acgaagcaag cttgtacaag ttctccttaa attcccgctc
     7081 actccactta atgggatacg cattgtactt ttccaaagcg gttattagct gattgtcaat
     7141 gcgccgatcc gaatcattga ggaaaacaac cagttcactt ggtgtctttc gtgccgcctg
     7201 cgtatcctcc catgaaaaca aggtaccgga aatgagtcgc tgggaaggcc acgtaataga
     7261 tgtgcagaat cgttccggct gtttactcgt gaacggcaac acaaagtcat acaggcacag
     7321 caaaccagac ttgccacgga actgaacgtc ttccacatac cgtatgttgt tcgcattaag
     7381 gaacatacga atgtcatctg caaaatacgt ctgcacatgg gattggccag catagacata
     7441 tcaccaactt tgatgatagc ttgcaggaac cgattcttgg catcagcaaa atcagcagta
     7501 tcgctcgtca gagtaagctc gtcgccgtta cgttcaatac caaactgatt cagaatatct
     7561 ttgagcactg ccaaacgcga ttccgtcaca gtcatcccat gcgactccaa acggcgtgat
     7621 gtggcacagt catcagagaa cgttacgctg ttcttcccgg gcttcacata gaactgcgta
     7681 tagtcgttgt cttcatccag aagtggaaca ttacttcata ccactcatcc agttgcgtat
     7741 aagtcgtgtt atcagacagc cattttccgt aacttcgaat taatgagtca aaatcagaac
     7801 tcgtcatact ttcttctcgt cgtaaacact acgattcatt tgactacaaa cttatctgat
     7861 gcaattggaa ttacttctcg cctttattca gggtgatgga cttcggagac agagcctcct
     7921 tcaccagttc cagcatacga cgagcttcag catctgtcag tacagccatt gacgcctctt
     7981 ccccgaatgc gattacccag tgggaacagc ctacatcagg tcagcgggtg ccgggtattc
     8041 ccgggttcta gatagaagcg caacacccgc tacaatcagc agtatggctg ttccgcaaaa
     8101 gccagcgttg gctcaattcg aagtcatgtt cgaaggcacg ccgctgattc tcgaacgcgc
     8161 caccgattac tgggaatcag gccgagtgag ttcggtgcag gagattgcgc cgcagctgta
     8221 tcacgcgcag gtgtccggca gtgccgatga ctgttatgac gttgacatcc gcctcagcaa
     8281 agacgctcac ggcgagaacg ccaatgaagc aaacaccgat gaccaagcca ctaccagcag
     8341 taacagcgac cacgaatcca caaccgacga caattcgatt cgcatacaat ccgccgcgtg
     8401 cacctgcccg tatcagagaa caccatattg caagcatata ggtgccgtgc tttacgaact
     8461 gcgcaaacag ctcattggcg cgcacggcaa tcaccacaat gatgacaccg ccaacgctca
     8521 cgatggcgtc atcgcactcc cccgcaccgt gctggatccc atccagcaac acctcgacgc
     8581 gcgcgcagag cagggcaaag gccatctgct gttcctgtgg tccacccaac tgcgcgaagc
     8641 cgcaaagacg ttctgcgcca agccagacga agaacgaccg ctaatcccgc tgatcgacgc
     8701 acaaaccatg ctctccgaca caatccgccg atgccatgaa cgtttcatta ttcatatcag
     8761 taagcacagc gatgcgtaca acaaatcatg tgactacaca gaattcatca aaaccgtgca
     8821 ggctatcgcg ggcaatgccc tgcaatccag tgattacgcg agcgcatgcg gcaacctgcg
     8881 actgtgcata cacgaagtcc acgcgcttct gctctcgtcc gagaacgatg atggctccga
     8941 acctttgctc acgcttatgg atgatctggc gatgcgcgtg cgatgctata tggagaatgt
     9001 ggcggaattc accgactcct ctaccgccgg caaggcattg aacaccattg cgcaggccgc
     9061 gaacgacaaa gacatgcggc aatgcgaacc attgaacagt atgttgctga tttccagcgc
     9121 gctcgctttc gcccaatatg acgacaaacg catatgggcc tatgacgtga tcgagaacgc
     9181 gataacgcgt aatctggaat actcgttcag cgaagagagc gaagaaagcg acgaggacga
     9241 cgaggatgag gataacgagg atacgagcga agtagacgac gaaaccgact tcattttgga
     9301 tgaatcgtta cacgtattgc agttgttcac actcatgagc gcctacgacc tatacgctct
     9361 atccaacgat gacgctggac gcgagcagct tctgaaagac tacccggagt caatggccct
     9421 cacgctgatg aacgctgcga acatgattca tgaagggcgc ttgcgttccg catacatgct
     9481 cgcacaagat nnnnnnnnnn cgagccggga tatggaagat gtcgatatcg atgcccggca
     9541 taacggtctg ctgccggacc tgctgcccca tggatggcat accatcatgg aatgctgcgc
     9601 cgagggactc aatgacgttg gcctgctcgc caacgtgtat cggtactaca ttctttcctg
     9661 caacgacaga tccgataccc attacgtgag taaactacgc aatctgttac gcatctacgg
     9721 cggtcttagc gcagaagaat ggcatgacgt ggctgatggg ctggccagag attgcgcccg
     9781 gaacatcatc gaccgtatca agtaccagcc ggaaataacc accaaaggcg gtacacaacg
     9841 tcacagcagt tggcgcaatc ccgcttatga gaagctcatc gtggatgagc ggctttcgga
     9901 cgcggccctg acctattgtc ccatcgtaga ttacccgccg ctgccattgc tacgcaccat
     9961 cgccatcgaa caccccgaat cggcaaaatc gataatcctc gaagcgatgc catacggcac
    10021 catgggcgcg cccgtgttcc ggtttacggt ggaacgtggc gtcgacaata cattgacggc
    10081 gcgccgcacc acataccagc agatcgccaa acagctacgt cgtttcgccg cagtgttcgg
    10141 cgatgaggag acccgtgtgc tggcacacga gattgttgag cgttatccga atcgtactgc
    10201 gttgcgggaa gagctggcgt ttgcgttgta gtgacgacat tggcaacgcg gtacggcatt
    10261 cggcacatat ccattgacta tgcagagaaa tgccagcctg ttcttcgctg agattcctga
    10321 tgatattggt cagctggatg caaacccaga
//
LOCUS       DAWBMW010000044        10191 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_1621_length_10191_cov_2.706196, whole genome shotgun sequence.
ACCESSION   DAWBMW010000044 DAWBMW010000000
VERSION     DAWBMW010000044.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 10191)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10191)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10191
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1621_length_10191_cov_2.706196"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4036..4045
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2203 a         3260 c         2826 g         1892 t
ORIGIN      
        1 ataagaagtc cgtggatgtg gatgtttccc agtggatcgc caatccttcc ggcacagcca
       61 atgagctcaa ggtcggcgtg catccctccg ccagcgctca cgctcacgtc aggggcggcg
      121 agcattcgac caccatcacc gtcgacctga cgtcacaggc gcgagccgtg ccttacacgg
      181 tgaccaatac gaaatacaac atcacctcca ccgcgttcat ccaggtaccg gcctatggtg
      241 tctttccgcc cacgttgcgt cccaaggcac cggaattgaa ggtcaattcg cgcgagacca
      301 ttgaaatcaa catcaatgac tatgtccgcg tcggcgccgg caaggaaccg tatatcgaaa
      361 gcgctgattc cgtcagcgcc accaaggcct ccaactccga tttctacgtg aacgacaaga
      421 ccctgaagtt cactgcggca aaagactacg caggccccgc ctccattacc tttaccgctg
      481 tggatggcaa gcagggatcg gataagacca agatcatcaa ttccgcagtg atcactctcc
      541 agatcacggt gataggccgc gatgtgccgc cgcccacgtt ctcgtcctcc actattgatg
      601 tcgaggcggg cgcggacccc aagaccgttg atctgacggc gctcacccat gcaccgtccg
      661 gcgtgtatga cgacgaaaag caatacacgt attccggtgg aagctcatca tccaggcaaa
      721 tcaccgcaaa cctgtcccgt tccggtagct tgcaggtttc cgccaccaag gatgccgctc
      781 ctggcaccac cgcgtccgtt cccgtgcaaa tcgcatacgg cgcgggcacc gtgaatgccg
      841 gtgtgaccgt gcgcgtagtg tcttcgaccc gtccgctgac acgcgtcggc aacaagacgt
      901 tgaagatcaa ggccggtgcc agcgatgccg tgaacgtgct taccggcgcg tacaatccgt
      961 tccccgattc ctcgctgacc gtcaacagct gcaaggcgga tgacgccgcc aaactcacca
     1021 tcgccggatg cgatgcctcc ggaaacatca ccatcgccgt cgcatccgat gtaggtgctt
     1081 ccacgaacac cgtgctcgtc accgtgcaag acggcaccaa gtccaaggat cgtgaagtca
     1141 ccgcatcgat taccgtgtcc gtgatcgaca agcccgagcc gccactgctc tcccccgtag
     1201 ccggggaacc gcaggacagc gcggtcagcc tcacatggac gcccggtgcc gccaatggca
     1261 gcccgatcac caactacaag gtcagttgga ccggtgccgt caccggagag aaggattgcg
     1321 gtaccgtcac ctcatgccag gtcactggtc tgaagaacgg caaaacctat tccttcaccg
     1381 tcgccgcgcg aaatgacgtc ggctggtcca acaccggaca aggtgccgtc cgctcccacc
     1441 gatgttacga tcaccggtgg caataaaacc gccagggtga cgtggaaagc gccgagcggt
     1501 gatttcagcg ccgtcgacaa ctattcagtg accgtcaccg gtgcaggtgc tccgcagacc
     1561 aaggaaaccg gcaatacggc caccgagctg tcgtttacgt tcaacaacaa cgacatttcc
     1621 gatggcaccg ccattacggc gaccgtcaaa gcccataaca aaatcaattg gagtgccgag
     1681 tccgccgcat cgccgtcaga gaagatctgg ggagacccgg atgcgccgaa cattgcgttg
     1741 tcgaacgacg acaccacggt aacggcgaaa gtcactctcg gcaataaccg caatgccggc
     1801 tgccggcgaa tttccttggg aggagacgtc aaggacacca tcgattgcag tgacagtggc
     1861 gctacgttcg atatcaatga gaacgatttg aacacccggg aaatcaccgt aacggccacc
     1921 gtggtgccgc agcgcgatgc ttcctccggc acgggcaaga gcagctttgt cccgcagtac
     1981 aaggtccagc cgcccactga cgtctacacc accggatccg gcagtacatg caccgtgcat
     2041 tggaccaagc agggacatgc gcagtcctac agcgtgactg ccgatggcat cgattccggt
     2101 acagctacta accggaactc actcgacttc cgaatgagtc cttgggccaa atgcaacacc
     2161 gcatcggtgc aacaggtgtt caacggcgct accagcgacg ccgtcaccgg aggcccgcac
     2221 aatggcgctc cttacaccta tgagaagaag gctgtaatca acaaaccgca ggagctgcgc
     2281 tggtcagaca acgctcacgt cattaacgtc accggcggtt cggttgatgt ctatggcaag
     2341 tcggccactg tacaaatcat catcaatggc caaccgttca cgtggaactc cggccagcca
     2401 ttggatgtca ccgacttggc cgtggccgcc gatggcaact acgcgtggag cgtcaaagtc
     2461 accggcgggc cgggatactc cggtctcgac aacactgcgg acggaggcag cgtcgcgggc
     2521 acacgtccct ctgactcctc ggaggggacc aagtcgctca aatccggtac cgatgtcacc
     2581 actttggcca agaacccctg gattcttggt ttcggcaata ccccaatatc gtcaaccacc
     2641 attcacaagc agtaggagaa catccatgag cgagaacatc gatgacgcga cgcagttgtc
     2701 tgacacgacc cgtctgtccg acagcacgca gctatccgac agcacgcact tgtccgacgc
     2761 cacgcacctg gcatccgcac cacacaccgg cacgacccca agcaccgagt cggcgccgca
     2821 gtccgttgcg ctgcctaacc tgccgcagcg gccgtcacaa gcgccgtcag aaaagccgtc
     2881 tcctactact gatgccgcct ccattcctgc gggcgatgac agcaccgtgc tcaagccgcg
     2941 caagggatcg acgccagcca tcccgcagca ggccaccgcg caatcccagc cgcaatcaca
     3001 gccgcagcgt cagtcgagca aagccgcgca agcagccgca tcggcactgc ccgccagcat
     3061 caattccgga ctactgcccc agcccacggc tcaggtccgg gatttccagc agtccttcaa
     3121 tgcgctggtc gaaaatgtct ccaaggtggt tatcggcaag accatcccta tcaagcaatg
     3181 cgtcaccgca ctgatggtcg gtggccatat tctgcttgaa gacaaccccg gcaccggtaa
     3241 gacccagttg gcccgaggct tggccaattc catttccacc ggtttcaagc gtgttcagtt
     3301 cacgcccgat ctgctgcctt cggatgtggt gggtgtgact ttctacgatc agaagcgcgg
     3361 agaatttgag ttccgcgaag gcccgatttt cgcctccatc gtgctggccg atgagatcaa
     3421 ccgtgcatcg ccgaagacgc agtccgcact tctggaggtt atggaagagc agaaagtcac
     3481 cgttgacggc gtcacacatg atgtgccgca gccgtttatc gtgatcgcaa cccagaaccc
     3541 catcgagcag ctcggtacct ataagctgcc tgaggcgcag atggaccgat tccttatcaa
     3601 gacgtcaatc ggctacccgg gccatgatgt gtccgtcgat atcctcaagc aggtcgatat
     3661 caccgaccgt gcgcagacca tttccccggt gctgagcggt gacgatgtca tgcggctgcg
     3721 tcaggtggcc accgaaatct atgtggatga tgcgattcgt gaatacatcg tcaggctcgt
     3781 cgaggcgacc cgccacaacg agaagatcac ggtcggctcc tccatgcgtg gcgctttggc
     3841 actgacccga tgcgcccgta tctgggccgc ggccgatggc cgtgcctacg tggtgccgga
     3901 tgacgtcaag gaccttgcag tggccgtgct cgcacaccgt atcacgctga ctccggaagc
     3961 caccttcgat ggcgcgactc cggaaagcct gatcgcacag gtgctcgaag acgttccctc
     4021 ccccaccatc ggttcnnnnn nnnnntcctg atagggcccg gaaaggtatc cgaccatggc
     4081 atctacatcc gccgcattac gccgtcgccg gcgcgctgaa cgcatgttca agcgcgcggt
     4141 aagacgcatc agacggttct tcgcatccta cgtctcgccc ttgggctggg cggtaacggg
     4201 cgcaggcatc gcatgcctta ttgcgttccc gtttctgggc tggtatgagc tgctcgtatt
     4261 cggcatcgtg gcgatggtga tgatgctcgc ggcaatcgtg ctttccctcg gcaacacacg
     4321 cttccacgcg tccatcgtcg tatccaatca tcgcgtgacc gtgggagaca ccgtatccgt
     4381 tatcgtcggc atcgacaaca ccggcagaac gccgacgacc acggcacgcg gatatctgcc
     4441 aatcggcgat gcgcatgaac gattcaacat ccccatgctg gctcccgggc aatccaagca
     4501 aaccgatgtg gagttccgta ccgtatcgcg cgcaatactg cccatcggcc cattgcgcat
     4561 ccgcaaggga gacccgttcg gtctggtgcg tcacgaaaag gagctggccg accgcatcac
     4621 cgtattcatc caccctcgta ccgtgcgtct cgatactctg aatgccggcg tgccccgcga
     4681 cctggaaggc caaccatccg gccagatcgt ggatgatgat ctcgacttct acggattgcg
     4741 cgaatacgag ccgggcgacg acgtgcgtaa cgtccactgg ctgagctccg cgaaaaccgg
     4801 cacgttgatg attcgccaat atgaggccac gcgccgcacc gacacctcgg tgaccatcag
     4861 cgtgaacccg gacgaatacg tcaacggcga ggaattcgag atggcggtgt ccatccactc
     4921 ctccatcggc gtcaaatgcc tgctgcagga tcgtccattg ttctgccacg cgggcaacgc
     4981 gcacggccag ccgcgcaacg ccatggagtt cctcgacaac gccagtgcca tagagcctga
     5041 tcgtgaggat aatccgaatc tcgccgaggg cacgctgaag cacagtcctg atgcttcgtt
     5101 ctattttttc actgttggat cgctgaaaga catcgagcaa atcaagcgca tgaccttggc
     5161 cttgccccga tcggccagct gcgtggtatt gcaggccagc gccggcgcca atcgcggcat
     5221 cagaagattc gccaacttca cattggccac catcggcgac ctcgatgatc tcccgctgat
     5281 catgggggtg ctcgcatgac cttcgaccag tccagtatca ccaataccaa taccgctgcg
     5341 tacgccggat cgagcggcac gtacaccggg acctccacca ccaccggctc ctgggccgac
     5401 tccacacatt cggttatctg gatgaccaga ggcaatgtgc ttcgcggcat gggcctcagt
     5461 cgccagccgt ggggcaaacg aatcgccagc ctcgtactca tagcggcgat gatgttgctg
     5521 gcgcaagcca atctcatcga cgtgtacggc ggcgtggcca cttgggcgat tgccgctgta
     5581 ccggccacct tgctgggcgc gatcatcgct ctggcgggca tgttgccggc gttgcgactg
     5641 tggtggcaaa tcgtgtttct ggcgtttgcc caattcatca tcggcccggt ggtaacgctg
     5701 agttccacta cgtcccatta cgtgattccc accttgaaga ccttgagttc aggttgggaa
     5761 atgacgtttg gctcgttcaa gtacattatt tccgtcgatc cgcctctggg cacgcaagac
     5821 ggcgtgctga tggcggtatg gaccatcggc ctgtggctta cgttcttcac cggcgtgttt
     5881 gccattaatg ccaacgcatg gttgtctctg gtgggagtgc tgccccttgc ggcggccgtg
     5941 gccgtatgtg ccctgctggg cacagacagc ggctggcaac gcgccatctg cggtattgcc
     6001 tttgccttgc tgctgattat ctggctgtct tggcgtcttg aactgctgga atgggggcgc
     6061 tggatatccg cgctgatcat cgtcgtgctc gctgccggcc ttgctttcgg gggcacgttg
     6121 ctggtgccac aggaccgttt cgtgctgcgt gaccgctacg atccgccgtt aagcccctac
     6181 gattacacca gcccgctcag cggcatgcga tcctatatca aggaccacaa aaaagacgtg
     6241 ttgctgaccg ttcataatct gccggccgga acaccggtca aacttgcggt gatggatcga
     6301 ttcgatggca cagtatggaa cctgtccgat tcttccgaag ccacggactc ctcgaattat
     6361 catcgcgtgg gcaccacgat caaggcggat gagcagggta aatccttcac tgcgaccttc
     6421 accgtcgatc agggactgac cgacacctgg ctgccgctgg cgggagcggc cactggcgtg
     6481 agcttcgcca acgatgccga caacggcaat gacacgtttt actacaacac ggataccgat
     6541 tccgccatta ttcccgccgg cacgcgtaaa ggcctcacct ataccgaatc cggcattatc
     6601 gcccgtaatc ccaccgataa gcagatcagc agtgcggctg cggcccgcat cacccaacca
     6661 gaagcccagg atgttcccga ttcggccagc aaactggcca cgtccattgc cggcgggcag
     6721 tcctcgggcg gtgccgcagc cacggcgttg gccgacacat tgaaagactc cggatggttc
     6781 tctcatggac ttgaaggcga ctatccctcc gatgccggcc atggcaacta ccgcatcaac
     6841 aagctgctgg caggcacggc catggtgggc gactccgagc aatatgcctc cgccatggca
     6901 ctgatggctc gtgatctggg actgccctcc cgtgtggtcc tgggcttcct gccgaaaaac
     6961 gaggatggcg aaatcaccga tgcccgcacg gagaagacct caggcaatgg caccaaaatc
     7021 gaattcaccg gcaacgatgt caccgcttgg gtggaaatca aattgcaggg cctgggatgg
     7081 gtggcgttct atccgacgcc caaggagacc aagatgcctg atgaaaacca gaacctgacg
     7141 ccgccgaatc cgcagacgct ggtccggcaa ccaccagtgc cgttgaccga tccgctgcgc
     7201 gatcagacgc aagccaaggg ccaaagctca ttggccggcg ccgacgccga tgattcgccg
     7261 accaacctgt tctgggcgcg gttctggcga gtgacccgca aggtggccct gtacggcagc
     7321 ccattgtgga ccctactcat cgcctgcggt ctgattctga tgttcaaggc catactgctg
     7381 gcacgagcgc ggcgacacgg cagtccgaaa acccgtgtgg cagcaggttg gaatgccata
     7441 cgcatgcttg ccgagcaaag cggcatcaca gccaagggca ctcgtcgaga tcaggccggt
     7501 gccatcgccc gtcaactcgg cacagatgac caggctttga tccagcttgg ccgggaagcc
     7561 gactacgcca cgttctcggg gcaagacatt gaatcgggcc aagccaccac atattggaca
     7621 ggagtcgacc aattgcgcaa ggcaatgctc gcttctttgc cgcgtttcag gaggatacgt
     7681 acacgacttt ccctgaaaag cgtggctata ctgtcaaatg ggcacgcagc aaagccgcac
     7741 acgacgcatg tcaagcgcac gcgtaagaag gggaaggcgt catgaccaca ggctccatcc
     7801 gtatcgcgat gaccagtgac gtgggtctta ggcgcaaaaa caaccaagac accggattcg
     7861 cgcaacaagg cgtgttcatg gtctgtgacg gcatgggcgg cggcaaaggc ggcgagcagg
     7921 caagccagtt ggctgcccag catttcgcac aattggccgc catgccttca cgcacccgtc
     7981 aggatatcga cgacaccttg gcacgggccc agcacgacat tctcgatctg ggagaccgcc
     8041 ttggcggcgt ggccggcaca acctgctccg gtttggtact tccccaagat catgcggatt
     8101 ccgccagcgc ccgcagcgct gctttgttca gtgacgccga tgatcaggac ttctcggacc
     8161 agacctatgt ggtcaatgtc ggcgattccc gcacctacca tctcagtccg ttggcgcatg
     8221 ccgatgctcc cgccacggat atcattcccg tgtgggacgc cgcttcgctg acccgcatca
     8281 cgcgagacca ttcccagcgc caagaagcca tcgattccgg gcagatgttg ccggaagagg
     8341 cggaggcgac cattccacgc aacatcatca cccaatgtct gggtgatccg gatggcatca
     8401 tgcccgacgt atatgtcgcc gatctgaccg gccgcttcat catctgttcg gatggattgc
     8461 atggcgaggt gccggacgaa cagatcgcgg ccatcgccgc ggcgcattcc agtccgcagg
     8521 aggccgtgga cgccttggtg gcggccgctc ttgatgcggg cggaaccgac aatatcactg
     8581 tgattgtggc cgatatgcca ttgaccgaac ccgaacatca tgcgttcagc gcattcaggc
     8641 tggacgaagg tgaggatatc ggcgccattg aagacgcaac actacaaaca ctacggacca
     8701 tacgttcggc ataaccgagc gaacataagg aggtaatccc atggttgatt atgatgctgc
     8761 ggtcgctgcc gttcaggatc cgaacgcgga tccgattctg ctggcaaaaa tcgcctacga
     8821 gaatccggaa ttcggtgcca atgtcgccgt gaatccgcgc tgttatcccg gcttgaagcg
     8881 ttggatcgcc gagttcggtg acgatcgcgc ccgcgagacc ctggcgcaat acgggttcac
     8941 tgccgaggca ttcggcggcc cggttcagga tcagaaagcc acggcacagg cggcccagca
     9001 gcccgccgcc gaacaggctc agcagcctgc ggctcaggcg gcattcgagg agcctgtggc
     9061 caccaatcct tacggtttca ccgccgaaca ggccctgacc accaccgacc agatgcaaat
     9121 cgcccagatt gcccagtacg cgcctgaact acgcgcctgc atcgcacgta atcccaacac
     9181 ctatccggca ctgatcgaat ggctgggcca gctcggcgac ccggcaatca acgccgctct
     9241 ggcttcccgt cagtgaaagg acggcatcat catggccaaa cttccctacc ctccagcacc
     9301 tgaactgggt tggtattctg acgatccggt ggaaggcacc ccggattccg cagttggcac
     9361 agccgaaccg cagaacgact acgggcagcc cagtaccgcg gaagcggcaa ccaataccgc
     9421 ttttacgcag agcctgccgc cacggccgcc gccgctgagc ccgctgccac cgctccggca
     9481 gacaccacac cgcacgccgc cgaaaacgac atcgaggatt gggacagcac cgtgctctcc
     9541 tcctcgttca cgtccaaggc accgaagaag acgtacctgc tgcataacga tgtcaccggc
     9601 cagaccatcg tgatcgataa gagcacgttg ctgggccgca agccttccat ggatgtgccg
     9661 cagggtgcca aggcggtgcg tatcgtagac ccgacccgca ccacatcccg caaccatgcc
     9721 gccatcagca tcgacaccga tggcgcgctg tggatcgagg actacggctc gttgaacggc
     9781 acctacatca tcaccaatgg ccaagagaca caagtcacca agggcacacc gctcaagctc
     9841 agcgcgcctg ccacggtgcg tatcggagac cagttcttcc agttcaccga aaaacaggct
     9901 tgagtaatca agcaacgagc ggctgcctgc tcttgttcaa caggaaaatc acaatggcaa
     9961 atatacggat ggcccggaac tgatatagtt ccgggccatc ttattgcgat taacgcaaga
    10021 tctgagtcct agaaggggca tcctttctag gaccttctca cttactcctc gatgtcaggc
    10081 tgaccgccct gatcggagta gaaatcatcc gggttgaagt cgtcgccgag cttcagatcg
    10141 ccgaactcga ggttcgagaa ggtcaggtca gacagggcgg cgtcggagaa g
//
LOCUS       DAWBMW010000045        10163 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_1627_length_10163_cov_2.384349, whole genome shotgun sequence.
ACCESSION   DAWBMW010000045 DAWBMW010000000
VERSION     DAWBMW010000045.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 10163)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10163)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10163
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1627_length_10163_cov_2.384349"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6747..6756
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7299..7308
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8002..8011
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1942 a         3051 c         3159 g         1981 t
ORIGIN      
        1 cgcagccgag gtagccgcgc ggagtcatca cttcaccgag aagcaacgca ccgcctaccg
       61 ccgagaagaa cgattcgagg ctcatcagca ggctggctcg cgtcggtggc acccattgct
      121 ggccgaccac ctgcaaggtg tacgcgatgc ccaccgagcc gatgccggcg tacagaatcg
      181 gaatccagga atgagcagcg ccggcccagt cgaccgagcc ttcgatcagc gaacccgccc
      241 agctcagcac ggcggtggtg cagaactggc cgaatgaaag cttgatggcg tccacggtgc
      301 cgcccaaagt atcgatcacc agaatatgca cggcgaacag cacggccgtg aacacgagca
      361 ggatatccgc caggccgatc gaaccgaaac catcggtgat gcacagcaga tagaagccgg
      421 cgatggacag cgccacggcc acgaacacca gcatgccgat gcgccggcgc aggaacacga
      481 aggccagcaa cggcaccatc acgatgtaca tggcggtcag gaatcccgca cggccggccg
      541 atttgccgta cagaatgcca tactgctgca gggtgctggc ggtgaacagc acgatgccgc
      601 agatcacgga aatgaccacc gggttggcga gcaatcgcgc cacggccgaa tcgggcttgt
      661 tcgcattcag cttggcaccg gtgatggtaa cgacgtcgga tgcaccgttc gatgcggcat
      721 ctgtggagac ggccacggat gcctcggtcc caacgacatc ggccccggct gtatcgccct
      781 tatccgcgct cccccggcca cgcatccaca gcagaatcgg caccaaagac agtgctccca
      841 gcgtgaagcg cgcggcgttg aagaacatcg gcgacatcga atccatgccc tggacctgcg
      901 ccacgaaggc aaaaccccag atcagcgcgg tcaacaacag gctcagcatg cctacgagtt
      961 cttgtctttt cacggggagc cagcctaacg tgcgaatgtg acagacatga aaaagcccgg
     1021 ccacaatgtg gtcgggctgt gacgtttgac gggcaggcta catgccgctc gccaatcggc
     1081 tgtcgaggct agtccctgtt gccgagcagc tgcaggatgt acaggaacat gttcacgaag
     1141 tcgaggtaca ggttcagggc gcacaggatg gagatcttct tgaccatttc cgggccctgg
     1201 gcctcgtatt cggtcagcag cgcgcgcgtg gactgcgcgt cgtagatggt catgccggcg
     1261 aacaggatca ggccgatggc gcagacgatc ttggtcatgc cgtcgacctg cacgaaggcg
     1321 agcacgatct gcgagatgat gagcacaatc aggccgatca tcaggatcgg gccggccttg
     1381 agcatgttga acttggtagt catgccgaac atggtcagcg caaagaagaa cgcggcggtg
     1441 acaccgagtg ccacgccgat cgagccgagg tcgtagacca tgaagatcga gctcaaggtg
     1501 aagcccatca atgcggcgta cacatagaac atgacgcgcg cggtggccga cttcatcttg
     1561 gtgacgcgca tgccgagcac cacggccagg gcaatctgca ccacgcacag gccgatgagg
     1621 cccaccatgc cggtggccat gaggaagctg tagtaggctc cggtgatctg gccgagcacg
     1681 gcgaccacgg cggtgacgag gatgccgatg gtcatctcgc cgtacacctt ggtcactgag
     1741 actcggcggg cttcctcata gttgtaggtc atcgtcgtgt tgatggtggc cgcacccgca
     1801 ccggcaccag taccctgcgg cacgtattgg taggtgggag cgccctgcgg ctgggcatag
     1861 tagttctggc cgtaaccgga ctgtgcgtat tgctggccat atccagactg ttgggcggcg
     1921 ttgtaatcag gcgtgttgta ttgcgggttg tattgcccgt tgttctgggg ctgctgaccg
     1981 aatgtcataa tcgctccttt agcgtttgtt ccagtaccaa tcttagagga acaaagctga
     2041 acattccatg aatatgatcg atcggctcgt tctgctcatc tcaaagcgta acgggcgttt
     2101 ccaccatgcg cgtggcatgc acttgagatt cgattgacat tcgatcgcag cttggaccgc
     2161 cgcgcgattc actgcaacgt gaaggtatgc gtcaccttgg tgtcttgatc cgttgatagg
     2221 aacgcagtga catccaccgt aatcggcgaa tcgtcttgca gcacatagcc cagcgtcacc
     2281 gttgccgcgg tcccgggctg tgcttgggcc aaatacgaat tcgcgtcata gccttcaggc
     2341 tgatcgaagt atactgccgt ctccagttcg gtgccgttct ggaacacgtt cgggtgagcc
     2401 aaagtggcga acgagttgtt cttggacgtg ttgttcgtcc attcataagt caccagagcg
     2461 gtgggcttgc cgttatagtc gttgacgctt ttcacggcgg agactatttt cacgtgggca
     2521 ccttcgatat caccttccaa atcctgctcg ccctgcgctt tctgctcgga tgccttgctc
     2581 ttggaggcat cagacgattt gcccgcggac tttgatgatg aggattgtga cgtgtcgatg
     2641 cccttggact ccttaatgga atcgtcaatg gccttgctgg cagcggactg catggccagc
     2701 gtgatcacaa tggccaacac cgacagcacc gccgccacaa tggacagcac cttgccgcgt
     2761 ttcttgcccc ggaaggtgcc cactatggcg ataatcgcca gtatgacgcc gatgaagcca
     2821 aggattgcgg cgatattgtt gacgatggga ataaaactca gcaccaaacc caacgcgcca
     2881 aacaccacgg ccgcgatgcc gcagatggtg gtatgcagat gttgcggttg cggcggctga
     2941 ggctgaggct gttgtggctg gtactgctgt ggggatgagg gaaccggctg cggggcgatg
     3001 ttgtggttgg acatgatgtg ctccttctcg tatcttcccc gaaataggga tgtgagcacg
     3061 acgctaccgg ccgcgaaaca gccacgttag tggacgaacg gtcattgttc gttgcctgtt
     3121 cgggcaatac ggaggtgata acggtgcccg catgtccagt ggcgggggcc ggatgcaggt
     3181 catacagtgg aaagcatgac gaacccacaa cgcgccgccg ggccataccc cggtttccga
     3241 cccggctcac ggcctgacgc tcgccctatg cccggcacgg ggtccccggt tcctacaccg
     3301 gatacggacg aggtgcccgt gactacgcgc gtatggtgtt cggtcgttat ggtgccattg
     3361 gccagtttca tgtgcatggc gcaaacctcg ttcttcgccc agaattttgc gcaggattcc
     3421 gcaggataca cgctgatggt cctctgcacc ttgctggcat tcccggcagg gttcctgctc
     3481 ttggcccgca gcgaataccc ggaggcgacg ttctggatcg cctgcgcgct ggtggtcgtg
     3541 ttcccgtatg actcgctcat cgcactgatg gcgatgacct cattgctcgc ccgtcgttca
     3601 aaccggaaca cgaccatccg cgccacggtt ggcggcacca tcgtgacgct catctcccag
     3661 ctccgcgacg ccctgcaaca gcccaaagcc tcgatttggc atctgatttt cgctcaaccg
     3721 cataccggcg gcgattcagg ctccccgatg gtgatgctgg tggaggagcc gaccgtcatc
     3781 atcaccgcca cggtggcctc actggtgttc gtgaccatcg ctacgctcat cggcctgcat
     3841 atccgttcgc gcgcccgcct gcgcacggcg aatgcggtgg ccagcgcggc caccacgcat
     3901 gccgcgaccc tgcagaccga cctgaccaac cagcagctcg ccgacgccat cgcagccgaa
     3961 gcgcacgaca ccctcgcgca ttccctgtcc ctgatggccc tgaacgccag cgcgctcaag
     4021 gcggaggcgg ccaagctcgg cgactccccc gaagcgcaat cgttggcgga taaggccgag
     4081 gacatccgtc ggcagtcggc cggggcattg gacgaggccc actcgatcat cgacatgctg
     4141 cgcaacccgc accaggcatg ggagcagctc gccccgtccg acgacacgtc gctgacccgc
     4201 gaatcgctgg atgcgctgat tgcggacacg cgcaactcgg gcacgtcgct caacacctgg
     4261 atcgatatcc agcagctgag cgtgatggat gagtcaatcg gcaaggtggc ctaccgcgcg
     4321 atccaggagg gattgacgaa cgcgcggcgg cacgcgcccg gagcgccggt gtcgttggag
     4381 gtctccgcca cgccgcaggc gggcgtacat atccatatca gcaatccgat cgccacggat
     4441 gcgaccgaga cgcagcgggc ccatggggcc ggactgcctg gtttggcagc gcgcgtgcac
     4501 tcggtcaacg ggcaatgccg gtatgggatg gacgatcggc acctgttcca tgtcgacgtg
     4561 caactgcctt ggcgcagcta agatggctag cgtgagtgag aagaacggga atagtgtgtc
     4621 tggtggaaag cccgaacgcg gcggtctgac cggaagcggc accggcttag agcccaagcc
     4681 gatccgggtc agcgtcatcg atgacgatcc gatgatctgc caggcgatga gcctgatcct
     4741 caacgattat tcccacgggc gcatcgaagt cgtctccacg tccaccgatg gcgagacctg
     4801 cgtgaggcgg gctgccgagg aaaagccgga tgtggtgctg atggacatcg ccatgcccgg
     4861 cgtggacggc atcgaggcga cccgtctgct gcgctcgctc tcccccgcgc cgcacgtgct
     4921 gatcctgacc tcgttgagtc cgtccggcac cgtggaacgc gcggtggagg cgggcgccga
     4981 ggggttcgtg tcgaaaaccg atgcggcgga cgatatcatc cgccgcatca tcggggtatg
     5041 cgagggcgcg ccgcagttca acacggccag tcagaaacag ctgatcgacg atctgagcgt
     5101 cacgcggccg cgttcgcgcc gcgatgaggc gcgcgccctg ctcgacgcgc tgcccgaccg
     5161 cgagcgcgag gccgtgatgc tggcggccga gggctatacg aacgccgaaa tcgcctcccg
     5221 catgttcatt tcggagcgta cggcgaaggc gcatctttct tccgctgcga acaagctcga
     5281 catgggccgc gtgcagatgg cgcggctggt ggagcgggcc gatctgccgg cgcggctgta
     5341 agtcgtccgg ttgttgagcc gtatcagtca taatgggaat atgagtgaag cgattattcg
     5401 aacattacgt agggatgact atccggcgct gatcgacctg gtgcgccgca cctggtatgc
     5461 cgaattcgat gagcggaccg gtctgctggc ggccgaagcg gactgggaga actgtctggc
     5521 gcgcaccacc aacgcgttcg tcgccgaact ggacggccag ccggtcgccc tgatcgtggg
     5581 gcgcgtcgat gcgctggacc atcggagccc gctcaactcg catcggatca actcgtggaa
     5641 cgcgctggcg cggctcgtgt tcgcgaaggg cggaatcaag gccgccggcg agattctggg
     5701 cattctcggc atcgacaaac ggctgcgcgc ccaggccgcg agcgccgggc atgactatgc
     5761 ggcggaagtg gtgctgttcg tacttgaccc ggcggcgcgc ggccacgggc tgggccggcg
     5821 catgttcgag acgctgatgg acgcgttccg cctggccggc gtgcgcgact acttcctgtt
     5881 cacggacacc acatgcgatg tcgggttcta tgtgcatcgc ggcctgacgc aggtgtgcga
     5941 gcagtcactg cccgcatccg tgccgggaga aaacccgtct tcgttctact tgtatgaggg
     6001 gtcggtgccc gagccggggt gatgtgcccg ccgtcgggca aacactggta ggcttgagca
     6061 caagttggta tgccggcata ccgtccacag gccggcgacg aaggggctga catggcaatc
     6121 agtgcgaagg aactaccact cgggaaagcg ttcaccccgg atttccggtt cacgatcccc
     6181 tcattccagc gggcctacat ctggaagacc gagaacatcc tgcaactggt aagcgacttg
     6241 gatgacgcct gcaagtcgcc cggtactccg tacttcctcg gctcgctaat tctggtgcgc
     6301 gagggcgata accgttacga cgtaatcgac gggcagcagc ggctggtatc cctgtcgatc
     6361 atcatcgccg tgttgcgcga tctggagcgc gatccggacc tcatgcgcca actcgatgcg
     6421 ctgctggtgg agcccggcga caagctgcgc ggcatcgtca ccgagcctcg cctcacgttg
     6481 cgcgagcgcg acgcagcgtt cttccgcgaa cacgtgcagg aagacaatct cgaatccgtg
     6541 ttcgatatga acgacgatga tctagcgacc gcagcgcaac gcaatatcgt agccaacatc
     6601 cgtaagacgt acgacgagat ttccaattgg tcggatgccg gacgccagca attcgcgcag
     6661 tatctggtca acgaggtgac gctggtcatc gtgaccaccg acgatctcga cggcgcgcat
     6721 cgcatcttcg acgtgatgaa catgcgnnnn nnnnnntccg acgtgttcaa ggcgcgcgcc
     6781 acggccggcc tgtcgaccgc cgaacttgac gtgtacgccg cccgttggga tgacatcatt
     6841 gacccgattg gcgacgatcc gcatgactgt gaggaattct tcgcctatct gcatctcgtg
     6901 ctcacgcaca agccggccac cgacaagctc atcgaggatt tcctggccga cgtgctgcaa
     6961 ccgtacatcg acaaaggcac ggtgcccacg ttcatcaacc aggtgctggc accctacgcg
     7021 atggcgtggc ggatcatcgc gcgccccagc gataccgtac tgcccgccga ggtgcgtagc
     7081 cggcttgagg gattggatga ttaccgcagc cacgaatgga agcccgtggc catgtgggcg
     7141 ttggtcaatt cgtatcgcgg gctgggcgac gccgatttgt cgccgtttgt ggttcgtgcc
     7201 tcgcgtatcg ccagaaccac ggccacgctg gaatcgttgg gtgcccatga cgagcgtcgg
     7261 ctgctggaaa ttctgaccgc attggaacag gtgaccggnn nnnnnnnnac ggcgaccgtg
     7321 aaggcgcatt ggtccgtttg catggcgaga tgcagggtga cggcgatttg gtccgtctgc
     7381 tgctgattcg cgccaacgag cagaaggccg gcatgcagtt ggatcgacca cgccggttca
     7441 gtgcgctgcc gattatgccg ttggatatcg aacggtcgaa atcgtttgcg gactggccgc
     7501 aggatcagca tgatttctgg atgtaccgac tcggcaatat ggcgctggtg cagggccctg
     7561 aggatcagtt ggacaggttg agtgaatatc cggcacgtcg cgaccgcatg ctgttgcgcg
     7621 cggattcgcg tcgtttcccg ctgaccaatc agttgaagga tttcgctgac tgcacacctg
     7681 ccctgttgga agcgcggcag gaagaggcgg tccgactcat cgtggaatac tggggcatcc
     7741 ggtatgacaa ggacgcccgc gatctgacca agcagaatgt cgatgaggtg tcgaagacct
     7801 ctccacggcc gtctcatagt tcgcggcgcg tcacgatccg gcaggtgatt gacgcgggtc
     7861 ttctggtgcc gggcgagcgt ctggtgtggg agcggccgcg caagggcgag cgctggttcg
     7921 tcacggtcac cgaaaacggt cgtctccgtc tggatgacgg ctccgaatat ccgactccga
     7981 ccgccgcggc ccgtgccgcc gnnnnnnnnn naagcagttc cgtctgcagg cgcagtagcc
     8041 ggcacatcat tggtggctcc cctccgtgag gggagccaca cgcttgtcta tgccagcgcc
     8101 gccacgattt cagccggcgt tttgcaatat tcgggaaaat gcttggctag ttttttgtca
     8161 tacgtcacca cataggtggc cccggattgt tgtgcggcgg cgataataag gtcatcctcg
     8221 aaatcgttat gcgcgtgccg caacgactgc gccaattcac attcctccgc gccaatcggc
     8281 acgattgtca tgagctcctt gagtttgctg attcccgacc atgccacctc cggcaatatc
     8341 agcttggcgg tggtcgcaac gaccatggat tcgtcattca tgaatcgttc tttgaatcgt
     8401 tccggggtga tgtagaacac gtccttcagc gacagtgacg cacaggacag ctttacctcc
     8461 cgctcgacgc atcgctcgaa cagtttcatg acatccttga tgaaccgagg cgtacagagc
     8521 atgtaataaa taaggatatt ggtgtcgcac aagatcgaca cctgctttga ctgcctcatg
     8581 acagactccg ctccttctcc aaataacgcg catagtccga ctgatgcctg tattccttca
     8641 gccgttcgtc agctatctca tcgcggaact gcttgtaggt aaggccttcc atgaggtcgg
     8701 tgtcttctga gatcagtccg gccttgacca gttccttgtg cacgtatccg gcaccttctc
     8761 gaatcagttt cagcttgcgc tgcttttccg cctcgcgggc ctgctcttcg gcggtcgttt
     8821 ccaatgccgg cacttccttg cggtcggcga tgtacaacca cagcgaccgg attacgtcac
     8881 tcaccgatac gcctttgccc ttgaggactt cttcgacctc gttcttcagt ttcgcatcaa
     8941 tgcgaacgtt catttggacc atggtcatgg caacctccat tccaaagatg ctatacagta
     9001 tagcatactg acgtgcatga aaaaggccgg catccgttgg cggataccgg cctttactcc
     9061 ccctcagtca gcttcgctga cagctcccct cagagagggg agccagaata agtcacttca
     9121 tgaggttgcg cagcacgtac tggaggatgc cgccgttacg gtagtagtcg gcctcaccgg
     9181 gcgtgtcgat gcggaccacc gcgtcgaact cggtcttgga gccgtcctcg tgggtggcgg
     9241 tgacgtggac cgtcttgggg gtcacgccct cgttgagctt ctcgacgccg gcgatatcgt
     9301 aggtctcggt gccgttgagg cccagggact cataggattc gcctgccggg aactgcaacg
     9361 gcagcacgcc catgccaatc aggttggagc gatggatgcg ctcgaagctt tcggtgatca
     9421 cggccttgac gccgagcatc accgtgccct tggcagccca gtcgcgcgag gagccggtgc
     9481 cgtattcctt gccggcgagc accacgagcg gcaccttgtt gtcgatgtag tcgcgggagg
     9541 cttcgaagat cgtggtcggc ttcttggcga ggaagtcgta ggtgaagccg ccgggcgtca
     9601 cctcctcgcc cacggaggcg agcagctggt tgcgaaggcg gatgttgccg aacgtaccac
     9661 gcaccatgat ctcgtggttg cctcggcggg aaccatagga gttgaagttc ttcggctcca
     9721 cgccgcgctc ggtcaggtac ttgccggccg gtccggaggc cttgaacgcg cctgccgggg
     9781 agatgtggtc ggtggtgacc gagtcgccca ggagggccag cacacgggcg ccgtggatat
     9841 cggcgaccgg gtccggcgtg gccttcatgc cgtcgaagaa ggtttgcttg cgcacatagg
     9901 tggacttgtc gttccacgcg aacagctcgc cttccggcac gtcgaggccc ttccagcggt
     9961 ggtcgccgtc gaacacggag gcgtagtctt tgaggaacat ctcgcggctc acagtgccgt
    10021 cgaccacggc ggcgacctcg gagttggtgg gccagatatc cttcaagtac acgtcattgc
    10081 cgtccgcgtc ggtgccgagc ggttgggttt cgaagtcgaa gtccatcgtg ccggcgagcg
    10141 cgtaggcgat gaccagcggc ggg
//
LOCUS       DAWBMW010000046        10104 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_1638_length_10104_cov_2.658075, whole genome shotgun sequence.
ACCESSION   DAWBMW010000046 DAWBMW010000000
VERSION     DAWBMW010000046.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 10104)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10104)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10104
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1638_length_10104_cov_2.658075"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1023..1032
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1790..1799
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1962 a         2866 c         3109 g         2147 t
ORIGIN      
        1 caactaccgt tcggtagtgg aaatcaactt cgttgaggaa ccgaaatatc gcgcaatcac
       61 tgcggacggt tatggcgcga ataccatcgt ccgcaatatt tccctgatcg acccagccaa
      121 aaagttcatt ccgggcgata cgctcatctt tttcgacgaa atccaggccc atcctgatat
      181 cgccactgca ctgaagttct ttaaaatcga cggcaaatat gatgtgattt gcagcggttc
      241 aatgttgggc atcagctata agcaaatcga aagcaatagc gtgggatata agaccgacta
      301 cgaaatgcga tcgttggact tcgaggaatt tctgtgggct cgcggatacg atgaggccgt
      361 catcgaaagc atgttcaccc acctgtgtga tgctacgcca ttctctgaag tggaaatgaa
      421 cgtcttctcc acggcattta tggattactg cgtgcttggc ggcatgcctg cggttgtcgc
      481 ctcccatata cgcactggca cgttcgaggg aacactgcaa ttgcagcgcc agcttatttt
      541 ggactaccag gaggatatcc gcaaatacgc gcaaggactc gatcaggcac gaattctcaa
      601 cgtgttcaac catattcccg tgcaactcgc caaggaaaac aagaagttcc aaatcagcaa
      661 ggttgccagc ggagcgcgtt tcaaagacta tcgaggatgc gtggaatggc ttgaaacctc
      721 gggaatggtg aatatctgcc gatgtatgtc ctatcctgaa ctgccgttga aaggcaatta
      781 tgacgaaaac aagttcaaga tatatttcgg cgataccggt ctgttcgtag cgatgcttga
      841 cgacgaggcc agtgatgatc tgcgggccaa tcgtaatctt ggtgtctata agggtgctat
      901 gtacgagagc atcgtctctg aagcgctacg gaaatccggc tatgacctgt actactacaa
      961 aaaagaggat tccacactgg aacaggattt cttcgtccgc acgcgcgaat ggctggtgcc
     1021 ggnnnnnnnn nnaaatcgaa aaaaggcacc gccaaatcca tgcggacgct aatcgcttcg
     1081 gattcttatc cggaaattca gttcggcatg aaactatccg ccggcaatat cggtagcagc
     1141 gaacacgtgt tcaccgcacc atacttcacc acatttttgc tccgacggct cctgcgcgct
     1201 tatgacgagg ggcaggcacc tatgctcaac aatcctgagc ggtagcccct attcgtctgc
     1261 gggcacggcc tggcccgggc aggtatggag gacggcgagc atggcgtcct tttcttggga
     1321 ggtgacggtg agctctcctg attggccggg ccgtcgacgg ccagcaggtt gtaggggtca
     1381 ttgccgaatt catggcgttt ggcagtggac cagtcgcgcg caccggattg ccaagcgttt
     1441 tcaagggcca ccacatggtc gatctgcact ttggcgctgg tggcgcggcc acgtacaaaa
     1501 tggatggtct gagcggtgta cggatcggcc agtgtgccgg actcgaccac acaggaaccg
     1561 gcgtatttgt aggtgatgtc cgtgagatca cgggcgagca cgtcgtcgcg tacgtcgcag
     1621 ccattgccgt cgacgtcggt ggtgcggaaa ccgaaggaat cgcggtcgta gccggaggag
     1681 ctttggttgt cgtctacggc gagcgcgttc aaagtgtcgg cggctgaacc ggtggcagtg
     1741 tagccacctg tgattttggc cgcgtcggag ctgacctgcg gcagtagcan nnnnnnnnng
     1801 cgagaacgat gagaatcagc acgcgctcca gcggaccaga agcgttgaaa cgacggcgga
     1861 agtgggcgtt acgacgtgcc atcaatcacg cataggtgaa ggtgaggggg tcgtggccgg
     1921 cgcgggtggg gccgtcgagg gcgtcgatgg cagcgtgttc atcggcggtc agggcgaagc
     1981 cgaacaggtt gagattctcc ttctggcggt cagcatgcac ggacttgggg atgatgatcg
     2041 tgccgttttc gatgtgccaa cgcagaatca cctgggccgg gctgacctca tgggcggcgg
     2101 cgatcttctc gatggtgccg tcgccggcgt tgaggtcggc gccacgcgcc atcggcgagt
     2161 aggcctccac ggcgatgccg tgctccttgc agaacgccac cacttcgcgc tgctgccacg
     2221 tggggtgcag ctcaatctga ttgacggccg gccatgcgcc ggtctcctcg tgcagacgct
     2281 tcagatcggc gggcatgaag ttgcatacgc cgagcgtgcg tgccatgcct tcgtcgcgca
     2341 gcttgacgaa cgccttccag gtgtcggtgc tgcgccagtc gaacggcgtg ggccaatgga
     2401 tcatgtacat gtccacgtaa tcgagctgga ggagtttcag cgagttgtcg aaagctttga
     2461 gcgcggaatc gtagccctgc tgggagtcgc gcagcttggt ggtgacccac agggtctcgc
     2521 gcttggtgcc cttgttgtat ccggaagcgg cgagcgcgcg gcccacgccg gcctcgttgt
     2581 tgtagccggc cgcaccatcg atatggcggt agccggcttc caacgcggat tcgacgacgg
     2641 ggaccacgcc ctcgtcgtcg atacgtagca cgccgagacc gacttgcgga atggaatgac
     2701 cgtctttcaa ggcgatgtcc ggcacgttgg cggggttgat gtgtccgttg gtcatggctt
     2761 ttgatgctcc tttattgctg cgactacacg ccactgtaca cggttatgca ggcgatgtgc
     2821 gggtgctgca accaaacggc atgattgcgc ccggccttcc gtgtgggaga gccgggcgca
     2881 atcatgacaa catcacgctc tactcctgcg accggccctc gccctattgt tcccgggcgc
     2941 gaatttgccc cggtacaggc gtcaatcgat tgacgtttgc accggggcag aaatcgttgt
     3001 cccggtacgc aatttcgggc cattttcgag aaccaggaca agacaatccc caaagtccac
     3061 ataccccgac aaacagaatc ggcggaatac agccaatccc gaaatcaaca tactgtcccg
     3121 gttctcacgt agaacgattg acgtgcaaac cggggcaacg ggaattgtcc cggtacgcga
     3181 aaaacacccg ttttcgcaaa ccaggacagt gatttctgtc ctggtaccca catcaaacga
     3241 ttgatgcacg aaccgggaca gaatcgcatc gctgcgccga gacagacgca tctgacagcc
     3301 gacaaccgat aaacgacagc cgacagatgg catctggcag ccggtcgccg gctgccggca
     3361 accgcatcat aatgccgcat cagtctctat caggagcgct tacgacgcaa agcaagcgag
     3421 accccgccgg ccacgccgag aacgacaacc gcaagaccca cggcagtcac cgcgctaccg
     3481 gtattgctca gcacaccagt gatcttgtcg ccattcgcac cgttggtctt atcgccctcg
     3541 cccgtatcgg gattgatagt agccccgcca ttacccgcac cgtccgagcc gttgccggaa
     3601 ccaccttcgg cccgagcggt aaccgcaacc gtataggttt cggtgacatt cggattcgac
     3661 ttgagcgtta cggtaacctt cgccgcatca gcgggagcgg agaaatcgta atcgagcgtg
     3721 aactggtcgg cggaaagcgt cacgttgccc atgcctgcgg aatagttgcc cacgaccttg
     3781 agatcatggt cggcatcgat ggcatcgccc acctgatagg tggttttgct tggcgtgacg
     3841 gtcaggctgg agacctcctt ttgggcgagg ccggcaatgg catcggcaac aagcttgatc
     3901 acatcgttca cgtcctgctc ggtgtaggtg ctgccggcaa gcagcacgtc agcggcggca
     3961 agcgcctgat cgagtttggc gagcgactcg ttggtgtact catcgcgatt gattttcccg
     4021 gcttcggcca cggccttgtc caggttctcc gtggatggct tcaccggagt ttcggtcttt
     4081 ttgacgagtg agaccttgtc cacattgccc catgcgtcgg caggcaggtt gcccgtgatg
     4141 gtgatggtcg ctgtgccatc agcacccacg gtgacaggca cggtggcggt atcgaactta
     4201 acccaaccgt tgagttccag cgcatcggac gtggtcttgc cacccgttgt ggcggagagg
     4261 gccacaccat cggtaatagc ggcgttgtcc gctgccgcgc cttggctcat ggcggtcagc
     4321 acgtattcgc caggctcgag tccggtgacg gtctgcgtgg cggagaaact gtaggcatcg
     4381 gatgcccaaa acttcagcgc gcgcttgccg tcggcggcgt tgccactgtc ttcggtgatg
     4441 gacgcaccgg tgccggcaat ggtccagttc ttatcgttct cagcgttctc gaagctgccg
     4501 tccgttacgt aatccttggc aggagtggcg gtgacggtga cggtgacggt cacgtcgtgg
     4561 ccagcgttgg tggtgccggt gatggtgtat gtgcctgcgc cacgaatcca gtcaagcgca
     4621 gcggattgcc aggtcacggt ttcggtctcg tccaccccat cctgatagtg tgcggccacc
     4681 tcactcggca attgcgcttt gatcgcgtcg atggaatccg agtcagtcgc ggtaatctcc
     4741 actggatcta tcttggtgaa cacatgatcg gtgacggcac cggtgtgaat gtacttgaac
     4801 gtcggcagag aggcgagcgc cttgccatag aaatcgaaca aagcttggtt atcgacacct
     4861 gagccgcccc agtacaggcc ggcgtcattg ggatcgtatt cgccggccgc ttcagtggcc
     4921 cagccgccac cgtacttgtt ccacgtatcc accagttcgg cgttatcctt gccgccggtg
     4981 cccacgggca cccatgcggg ctcccagtag aacacaccca ggccgtcgtt ttcgccatcg
     5041 ccatcattgt cgccgatatt gttcacggcc tcggcgaccg cgcgaatctc atcggcctgg
     5101 ccttgcgggc tgatgtcgta tttcttgagg ttatcggcgg ttactttact gggcactgtg
     5161 ttggaatcgt cgtcgccatc atccaaggtg tatgcccacg acgtttccgc caccatcaca
     5221 tctttcttat aagtggaggc cacgttcttg agcacgctgg tgaggttttc ggtggtgcca
     5281 tgccagaacg ggtagtagga gctggcgaac acatcgtaat ccacattgtg gttgctcaac
     5341 tgtttggcgt aagtggcgta ggtgccggct ttctccgggt tggtgaaatg aatgacgacc
     5401 ttggcttcgg ggagcacttc gcgtatggct ttggaacctg cggagaatac tttggacatg
     5461 ccgtcccagc cggagatgcc ggcgattttc gcagtggttt cgttgccgac ctgcaccatg
     5521 ccgacgtcca cgccggcttg tttgaacgtg gtcagagtct gcttggtgta gtcgtatacg
     5581 gtgtcggcgg ttttgtccgc gtcgccttca aaggatttcc acgccttggg cacctgctgc
     5641 ttgctggggt cggcccagaa atcggagtaa tggaagtcga ccagcacttt cagccctgct
     5701 gccgtggctc gcttggccat ggtcagtgcg cgatcggcgt tgacgttgcc gccgccatat
     5761 ccttggccgt ctgcggtgaa cggatcgttc cacacacgca gacgcacata gttcacgccg
     5821 gattccttga gcaacgtgaa caaatcctcg acctcgccat tggcgttctt gaacgtgacg
     5881 ccgctttctt ccagcgagag catggatgag acgtccatac cgccgatgaa atcatcgctt
     5941 aagccttgga tggccttgac cgagacgtcg gctttaactg gttcttcttc ggcggcgttt
     6001 acagtgaacg tgcctcctgc gcagagcatt atggcggcca cgccggatgc cataatgcga
     6061 gtaagcgcgg tatttcggcg catgcttttt ctcctttgaa tggtgaacgc tcagaacgaa
     6121 cgcccagtga tgcttggcgc ttcctttcct tatgcgccac gcggtacagc tgtgtaccac
     6181 gcaatcgcca ccttagcact ttatgccgtg ttggtcaacc ggtgtatcaa cgcctcgtac
     6241 ggcccagtac agcaacacca gcattacagc aatgtcgttc agcgtgtgga ggcgcgggcg
     6301 atgacctggg attcggtggt gagcagttcg gacttggtga tgctgccgtc gatgcgtccg
     6361 aggatgcgat tcactgccgt gggagccgtc cagtcaaggc gggaatccat ggtggtcagc
     6421 ggaatctgca ggtagggggc ttcctcgttg ttatcgaagc cgatgacctg cacctgcacg
     6481 ggcacctcat aaccagcggt gcgcagagcg gtcaatgcgc cgatggccaa ctgatcgttc
     6541 aaggcaatca caccgtcgaa tggcacgccg gaatcaatga gccgctgggt cacacgggca
     6601 ccggctccga tggtccaatc ctgatcggtg ttgccaatca gctgcggatc caaagagagc
     6661 ccacgcctac gggtctcttc gatgatgccg cgcagacgca gctgcgcgtt gccttctacg
     6721 gcattcagca atgcatcttc gtcgtaaacg tcacgggcac cgatcactgc aagtctggtg
     6781 gaaccgcggt catacaggta gcctgcggcc atggcggctg cggatacatc gtctggggtc
     6841 acgtgatcca caatgcccca cgtggtacgc gcgccaacga tgaccagcgg aaaatcaacc
     6901 ttcagatcat cgggcgaaat gttttccacc tcgctcatgg aaaggatcat gccatcggac
     6961 accgtggagt tgaacttctt cagcagatcg cgcgcgcctt tcgcggagcc ttccgcatac
     7021 gtggtgacgt atacggaata gtcgcgctga cgggcggcct cgatggtgcg gttggcgagc
     7081 tcggccagat atggcggaat cagcgaaggc acggcgaggg tgatgaagcc ggtgtgatcg
     7141 cggttgaggt tgcgggcggc cacgttgact cggtagtcga ggtccttaat gaccgactcg
     7201 accttggcac gggtctcatc tgtcatacgc ccggagccat tgatcacgtt cgacgcagtt
     7261 ttgagcgata caccggcggc ctgagcgaca tcgcgtagcg tcactcgacg cttggctgtt
     7321 ttctcgtcgc tcatgccgca ctcctctatc cggacaacat tgccattcta gcgcggttta
     7381 gcaggacgtt ttagcgataa gaatggcgtt gcggttcagc gtatatccaa cggcgtcagt
     7441 ctcgcaacgg tgggcgatga tgggatcacc ttccacaccg ttgatggcga cgggctggtt
     7501 tgagcggttg aggtagaaat cgaagcgggt ggagccgtct gcggatcggc ggatggtgtg
     7561 caggatgcgg ggatctgtgg tgggataggt tggagtttga ccactctcag tcggctgcgc
     7621 cgacaactcc tgccatcggg agccaagtgc tttgagcaac tgggcaatgt cgtgacggtt
     7681 gaggtcgcag cccacgtaga tggcggtacc gttgccatag gggcggctgg tgatggcggg
     7741 ggtgcgctcc agctcccagt tggcggcgga ctcacctgcg taagaggcaa gcactgtggt
     7801 gtccgcggct acggaagtca cgtcgttctg ccacaggcgg gtcgtcagac cattggacag
     7861 gctgatttcg ctgggctcgc cttcggcttc ttctccgagg atgttgaatt cttcggagcg
     7921 gatgccgagc atgtcgcgta ggagcccgtt gccggcgccg gggtagccgc cgaggccgat
     7981 gtggaagtgc tcgtcgatca ggccggcggc atagccgacg atgacggttc cgccggcctt
     8041 cgcgaagtcg gcgatacgga gaacgtcttc gtcggacagg ctgagcacgg tgggcagcac
     8101 gatggttttg tagccggtcc agtcgtaggc gagcggcacc acgtccgcgc gcagcccggc
     8161 atccaggaag ccgcggtacc agtcgcgcac gtcgtgccag tggttgagtt tcatgctggg
     8221 cagggtttcg gagcgtgtgg cccattcgga ttccgcgctg aacaggatcg ccgtgccggc
     8281 acgctcgagc tcagtgccct gcagcccggc gtcggagagg gtcttcagcg ccttaccgag
     8341 ctcgcatacg ccgcggaaga ccttggaatc ggcgcccgcg tggggcagca tggcggagtg
     8401 gaacgcctcg gcgcccgact tggattgccg ccattggaaa aagcagatgg cgtcggcgcc
     8461 catggccacg tgggccaggg agtcgcgcat gagttcgccg gcccgtttgc gtgtgttcag
     8521 cggcttccac tgcacggctg aggtggagtg ctccatcacg taccacggct tgcccagcgc
     8581 gagggagtcc atgagcgcgt ccgagcaggc gagttcgtcc aggtgggatt cgccctcatg
     8641 gaagtagtga tcgttggaca cgaagtccac ctcatccgcc catttggcgt agttcatgac
     8701 gcactggtcg gtggagacca tgaagttcgt ggtgaacggc ttgtcggggc agatctgctc
     8761 gatggcgtcg cgttcggctt tgtagaagtc gagcagcatg tcgttgccga accgctcgta
     8821 atccaactgc tgcgacgggt tgaccatggc gtcgccgccc atgtgacgtg gcagcagcac
     8881 ctcgtcgaaa ctgttcacgt gctgcgacca gaaggccgtg ccccacgcct cgttcaacgc
     8941 gtcgatggta ccgtacttgg cctcgcacca cgtgcggaac gcggccaagg cgttgtcgga
     9001 gtagtcgtag cggttgttcc agccgtattc gttgcccatg tgccaggcgg tcacatacgg
     9061 gttgtccttg tagtgttcgg caagtttgcg gcacaggcgc agcgcgtatt ccttgaacac
     9121 cgggcttgtg ggctgccacg actggcgtga gcccgcgttg acggtatgac cataccggtc
     9181 taccggcagc acctctggat gggattcgta cagccacagc ggtgcggcgg ctgtggcgga
     9241 ggccaagtcc acggcgatgc cggctctgcc gagcctgtcg atcacatggt cgagccactc
     9301 gaacgtgaac gcgccctcga ccggctcgat cttgtcccag ctgaagattg ccagcgatac
     9361 gacgttcacg ccggcctcac ccatgagacg gatatcctcg tccaacgtct cctcgggcca
     9421 ctggtccggg ttgtagtcgc cgccgaacgc gatgccgcgg ccggattccg tcagcaggga
     9481 aggccaccta aatgctctac gagtagtcat attaatcaaa tcctttattg tatcggtttg
     9541 cgggaagtgc tgtgcttgca ggttgtgaac accagagagg ccgccccgct ggcggggctg
     9601 tcagcgaagc tgactggggg tggtcgccag tcgaatcccc atatgaccac cctcagtccg
     9661 ctccgcggcc agctcccgtc agggggagcc agaggttact ccttgacggc gcctgcggcg
     9721 aggcctgact gccagtactt ctgcaggcac aggaacgcaa tcaccagagg gatgatcgtg
     9781 atcagcgaac cggtaatcac gaggttttgg atagcttgac cgcctgcggt tgaagcctgg
     9841 tccttccact ggttcagacc gatcgtcaac gggtaccagt tggaatcctt caacatgatc
     9901 aacggcagga agtagttgtt ccacgtcgct acaatggcga acaatgcagt ggtcacgatg
     9961 ccgggcgcca acagcggcag ggaaatctgg aagaaggtgc ggaactcgcc ggcaccatcc
    10021 acgcgcgcgg cttcaagcag ctcatccggc acggcctgat cggagaagat ccacatcaga
    10081 tacagaccga acgtggatac caaa
//
LOCUS       DAWBMW010000047        10065 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_1648_length_10065_cov_2.779021, whole genome shotgun sequence.
ACCESSION   DAWBMW010000047 DAWBMW010000000
VERSION     DAWBMW010000047.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 10065)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10065)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10065
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1648_length_10065_cov_2.779021"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    7785..7794
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8539..8548
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1806 a         3117 c         3176 g         1946 t
ORIGIN      
        1 agccggagta ggtgtgcagc acgtaccact tgccatcaag ggaacgcagg gacttggaga
       61 attcgtcaac ggcctgctga ccggcatcgg tctcctcggc ctgctcggat tcgttgactt
      121 cttcctcgtc ctcatccaga gaaacggtct cggtctgagt ggaagcaacg ggagcggatt
      181 cagtcgccgc cggagcgtcg gactcaacat ctgcattgtc gacggatgcg tcagattctg
      241 cggaaaggtc agcgaccggt tcgaccgggg cagctgcctc atcggcagca gcttcggaag
      301 cctcgacaac aggctcctca gccgggacgg cgtcgaggtt ctcgagattc agttcatcac
      361 tcatgcgttg ttattcacct taggttgtgc ggatcagccg aacacccaca gcgtggcctt
      421 gcctagaccg aagtccatgc cagtcacgag ggccatcagc agcagcacga agatgaatac
      481 ggccagagac cagaagaaca gttccttggc ggtaggggtc acgaccttgc gaagctcgtc
      541 gatgatctgc ttgatgaaca gaccgatacg catgaagaca ttcggcttga ctgccttttc
      601 actcttgctc gtcttagcca ttccgtacct cgcgtcagct gcttgattat tagaaacttg
      661 gcagggaagg cgggactcga acccacaacc tacggttttg gagaccgttg cgctaccaat
      721 tgcgccactt ccctaggtga aaatccacct cggcgtgcgt tccgtagaga acgcttcgcc
      781 acggcgaaac cgccaagtcg ttatagtaac ggctccggtg gattatcgca aatcgcgtgt
      841 cgcgcgcgta gcacgcgact cttctcagtt agcccattcc ttgaatcgac gcatgccttc
      901 ggctaggtct tcgtcggcca gtgcgtagga gaaacgcagg taaccgggag caccaaaggc
      961 ctcgccgggg acggcggcca cgtgggcctc atccaacagt gcagcggcaa agtcagcgga
     1021 cgtatcggac actgtgccct tgggacccag cggcttgcca agcaaagcgg tgatgtccgc
     1081 aaaaacgtag aacgcgccgg tcggagtcgg gcagttgacg ccctcgatat cgttcaaggc
     1141 ggtcacaatc gcacggcggc gcgcgtcgaa ggccttacgc atctcgtgca cctcatccag
     1201 cgggccggcc acagcagcca aagcagcacg ctgggagatg ttggccacat tggaggtcat
     1261 atgaccctgc agcttggcgg cggccttggc cacctcaagc ggagcgacca tccagccgac
     1321 acgccagccc ggcatcgcat aggtcttggc cacgccgttc agcaccagca actgcccgcg
     1381 gcattccggc acggcggcac cgatgtaggt ggtgtgcgca tcgtcatagt tcaggtgctc
     1441 gtagatctcg tcggagataa cccaaatgtg gtgctcgacg gcccaacgac cgatcgcttc
     1501 cacggtttcc ggcttccaga cggctccggt cgggttgttc ggggagttga cgatgattgc
     1561 cttcgtatgc tcggtacggg ccgcttccag cgcctcaagc gagggctcga agttcacgtc
     1621 ggcaccggcg aatacttcga ccggcacacc accggcaagc ttcacagcct cggggtagct
     1681 ggtccagtac ggagcgggaa tgatgacctc atcgccatcg ttgagcaaga tctggaagga
     1741 ttcatacacg gcctgcttgc cgccgttggt caccacgacc tgatcggcat tgacctcata
     1801 gccggaatcg cgcagcatct tagccgcaat cgcttcgcgc agctcgggca ggcccggcgt
     1861 aggcgtgtac ttgtaattct tcgggtcaag gcaggcatct gccgcagcct tgaccacgtt
     1921 tgcgggggtc gggaaattcg gttcacctgc gccgaagccg acaacgtcaa gtccggcggc
     1981 cttcatggcc ttggccttgg aatcgacggc gagcgtagcg ctcggtgcca cagtgttgat
     2041 acggtcggaa agagtctgcc attgcgccat agtcatggct cccatgctaa acctacagca
     2101 cgaaaaatcg ccagagccgc actatggtga tggaatctgt cgtgtgacag attccatcag
     2161 tcacacgagc acgagattgt cgcgatgaac cagtgggtgg gcgtactggg ggccgaggaa
     2221 acgcttgagt tgggcggtgt tacgcccgag catctgcgga atttcttcgg aatcgaagcc
     2281 ggcgaggcct cgggccaggt gttcaccgct ttcggcatcg atccagaccg gatcgccggc
     2341 ggaaaaatcg ccatgtactt cgagcgcgcc ggccgccagc agcgaggcac gcccgccgcg
     2401 aatcgcctgg ccggcacccg catcgaccac aatcgtgccg cgcgggtcgg cggcgaagcc
     2461 gatccacaga cggcgtgagg agccgcgggc cttaaccggt gcgaacacgg tgcccaccgg
     2521 atcgcccatc atcgcagggc ccgcgttcga ggcgcaggtg agtaccgtgg ggatgcccga
     2581 gacggccgcc acgcgcgcgg cctcgagttt ggtgaccatg ccgccggtgc cgaccttgga
     2641 gccggagccg gagacttgga tgtcgcccag cgcatcaatc acgttgggca catattccac
     2701 acggcgggag ccgggctgcg agggcggcgc agtgtacagc gcgtccacgt cggtcagtag
     2761 caccaaggct tcggcccgca ccaggttggc caccaacgcg gagagtcgat cgttgtcgcc
     2821 gaagcgaatc tcgttcgagg ccaacgaatc gttttcgttg atgatcggca ccacgccgag
     2881 gtcaagcaga cggtccagcg tgcgttccac attgcggtac tgggtggcac gaatcgtatc
     2941 ttcggcagtg atcagaatct ggccgacgcg aataccgaat cgaccgaacg cagtctcgta
     3001 gcgggccatc agtagccctt ggccgacggc tgcggtggcc tgctgagtgg ccacgtccac
     3061 cggccgcgag tcaaagccca gaggcccgaa accggcggcg atagcaccgg acgagaccag
     3121 caccacacgt ccgcccatca atcggacctg agccaaggcc gcggccaacg cgtcgagctt
     3181 gtccgggtcg aggtgaccgc tcggctgggt cagcgagctg gagccgactt tcacgacaat
     3241 ggttccggct gcggcgatca tgcgccgcac ctcggcctga ctcggagtgc tcatttattc
     3301 ctcggtttca ccatcttcgc tggactcacc gtggccgaac aggctggttt cgtcatggcg
     3361 atcgtcgtca acggtcgggt cggcccagtg gccggccttg cgttcggcca tcatcgcgtc
     3421 gcgcacggcg gcacgggcgt ccatcatctc gtggtattgg gcacgacgtt cggcgttgga
     3481 gcggcggtgc gtgcgcgggt ccagctcttc gagacgcaga tccttgcctc gggcaccgag
     3541 gttggagccg tcgagcattt cggcaccagc ggagatggtc ggatcccaat cgaattcgac
     3601 catacgggca ccacgaccga tgcgcacttc gtcgccgggg tgagcaccct tgcggcgcag
     3661 ctcgtcctcc acgcccagct tggccaagcg gtcagccagg tagcccacgg cctcgtcgtt
     3721 gtcgaagttg gtctgcatga cccagcgctc cggcttgacg ccacgcacct cgaagaacac
     3781 ttcgccattg ccgagttcgc ggcgctcgac cgtgaactca agcgcactgc cgccctcgtc
     3841 agcacgacgc ggacggcggc ccttggtctc caatggcttg atgaccacgc gagcctcctc
     3901 ttcggcctgc tcgcggttgg ccacttcctc acgcatctcg tgcaccagcg cagacaacgc
     3961 gaagttgagc tcctttagac cctcgtgcga ggcagtggag atctcaaaga ccttcagccc
     4021 gagtttctcg aactccggac gcacgaactc agccaattcc ttggcctcgg gcacatcgat
     4081 cttgttcaga atcacgatgc gcgggcgttc gggaatcggg atggcaccca acggcagctc
     4141 gagcttgtcg gcgtagagcg ccaactcgtt ttccagcgcg tggtagtcgg acatcgggtc
     4201 gcggtccggt tcaagcgtgg cgcaatcgat cacatgggcg atgatttcgg tacgttcgat
     4261 gtggcgcagg aactccagtc ccaggccctt gccttcagag gcgcccggaa tcaggcccgg
     4321 cacgtcggcg atggtgtatc gagagtcgcc ggcgataacg acgccgagat tcggcaccag
     4381 cgtggtgaac gggtagtcgg cgatcttcgg cttggcggaa ctcatcgccg caatcaggga
     4441 cgactttccg gccgagggga aaccgaccaa cgccacgtcg gcgatggact tcaattccaa
     4501 aatcacgtcg cgctcttcac cgagttcgcc gagcagggcg aagccggggg cgcggcgggt
     4561 cttgttagcc agggcgatgt tgccgaggcc gccggcaccg ccttgggcga cgacgcagcg
     4621 gtcgccctcg tggcgcaggt cggcgagctg cgcgccggga tgcttggctt tgccctgttc
     4681 gccgcgcgcc tcaaagacga cggtgccgca gggcacgggc agaatcaggt cctcgccctt
     4741 ggagccgtcc ttattgtcgc ccaaacccat ggtgccgctg ccggcgacac gatgcggcat
     4801 gaaacgatag tcgagcaggc tggtggcgtt acggtcggcc acgaacacca cggaaccacc
     4861 gtcgccgcca ttgccaccgt tcgggccggc caacggcttg tatttctcac gacggatgcc
     4921 ggccgaaccg ttgcccccat cgccgccctt gacatggacg gttactcgat ccacaaaatc
     4981 actcatattg attaccttac aaaaaagccg cgtccttacg ggcgcggctt gaaaaaagct
     5041 tgacgctata tcgctcaggc gatgacgtcg acaaccttgc ggtcgcgacg cacaccgaac
     5101 ttcacggagc catcggtgag ggcgaacagc gtgtgatcct tgcccatgcc gacgttgtgg
     5161 cccgggtgga agttggtgcc acgctggcga acgatgatgt tgccggccac gacggcttcg
     5221 ccgccgaact tcttcacgcc gaggtattgc gggttagaat cgcgaccgtt gcgagagctg
     5281 gacgcaccct tcttatgtgc catgattcat ggtcctttct ataactcagg caatcgccgt
     5341 gatcttgacg atagtcagct tctggcgatg acccttacgg cgagcaacac cagtcttgtt
     5401 cttgtacttc tggatgctaa tcttcgggcc cttggcttcg tcgttcacaa cctcagcctt
     5461 gacggacacc ttggccagat cggcggcggc aagggtgacc ttgtcaccat cgacaacgag
     5521 ggagaccggg aattccacgg tatcgccctt cttagcgtcg agacggttca cgaggatgac
     5581 gtcgccaacc tcgacctttt cctgatggcc accggccttc acaatcgcgt acattatcgt
     5641 tccttttatg ttttggaaag ctaccttgcc taacacgcac cgagcttgcg gtcagacacc
     5701 gagtttcgaa tctacaccac catgcggact aaatcaaatg atttttgccc gcgtgtcaag
     5761 cacgacacac ctgttactct tccgcttact cttccgccgt gttgttggca gcgacggcag
     5821 cggcggcaat ctgagccagt ttggctttga cgtcggcgct ggaaccagcc ggttcgggag
     5881 cgggagcggt gccgcgaccg tgcttgttgg ttttgacgaa cgggtcgcca ccgcgaagcg
     5941 cgtagggatc gtcgtagtcg gccgaaacgg tcggctgatc atgcaggatg aaaccacggc
     6001 ccttgcaggt cgggcattcc tcggagaacg cctcgaccag accctggccg atgcgcttgc
     6061 gggtcatctg caccaaaccg agcgaggtga cttcggccac ctgatgcttg gtgcggtcgc
     6121 gtgccaagca ttcgaccaag cggcgcagca ccagatcgcg gttggcgggc atgaccatat
     6181 ccacatagtc gatcatcacc atgccaccga tatcgcgcag gcgcagctgg cgggcgatct
     6241 cttcagacgc ctccaggttg caacgcgtca ccgtttcttc caaggacttg cccttgccga
     6301 taaagcggcc ggtgttcacg tcaatggtgg tcatggcttc ggtgcgatcg atgacaatcg
     6361 agccgccgga cggcaggtag acctgacgct ccatgccctt acgcagctgg gagtcgatgg
     6421 accacttgtc gaacacgtcc ttgccttcat gctcggccgg gtcccacttc tccagcttgt
     6481 ccttcaagtc gggggccatg gtgtcaaggt attcctcgat acggtcgtag accttgtcac
     6541 cttccacgat gagcttgctg aagtcgtcgt tgaagatgtc gcgcaccacg cggatggcca
     6601 cgtcgggctc gccctgcagg agcttcgggc gcttaccgtg ccagaattcc tcgcgcttgg
     6661 cgttgatgcg ctcccactgg cggaccagtg attcgagatc cttgacgatg gcgtcttcgg
     6721 atgcgccctc ggctgcggta cggatgatca cgcccatatc tttcggggcg atcttggaga
     6781 cgatgttctt cagacggctg cgttcgcgct ccgaaagctt gcggctcacg ccggtcatgc
     6841 cgccggatgg cacgagcacc aggaagcggc cggcgagcgt gacctgagag gtcaggcggg
     6901 cacccttgtg gccgatcggg tccttagtga cctgcacgag caccgggtcg ccggacttga
     6961 acgccagctc gatgcggcgc ggctggcctt cgagacgagc ggcatcccag ttgacctcgc
     7021 cggcgtagag cacgccgttg cgggcctggc cgatgtccac aaatgcggcc tccatgctgg
     7081 gcagcacgtt ctgcacgcgg ccaaggtaga tgttgccgac ggtctgtact tcctgaatgt
     7141 cggagacgta atgctcgacc agaacattgt cttccaccac ggaaatctgg gtgtgatgtt
     7201 ccttctcacg caccaccatc aggcggtcca cgttctcgcg gcgtgcgagg aagtcctgtt
     7261 ccatgagctg gctctggcgg ctgcgctcac ggcggttgtc acggcggcgc tgcttcttgg
     7321 cctcgagacg agtggagccc tccacatcag tgatctcgtc gatgtactgc tgcttgcggg
     7381 agcgtcggac cagcggctgt tcgctgttct gactctggtc gtcggtgccc tcgccgttct
     7441 tgccgccacg acggcgacgg cgacggcgag taacggtctg ctcctcatcg tcctcatcgc
     7501 gatcacggtc atgatcatcg cgatcgtcac gacggcggga acgcgtatct tcggcatcgt
     7561 tctcggcggt atcgtgctcg ccgttggcac cggtttcgcc gcgtgagccg cgacggcggc
     7621 gacggcgcta acgggtgcgg gtggcgtcgg attcttcgtc gccctcctct tcggcaatcg
     7681 gcgagtaggc gatatcgtcc agctcgaggt cttcctcaat ctgctcgact tcggcggctg
     7741 cacggcgctc ctgggcgttc aggcggcggg agcgacggga acggnnnnnn nnnncatcac
     7801 ggtcggcggc attgcggcca cgatcgtcac ggtcgtcacg gcggcggctg cgcggctgcg
     7861 cgtcgtcatc atcgtggctg cgcgagcggc gggagcgacg cgacggacgt tcgtcgtcgt
     7921 caacatcgtc atcacgagtg tcagcggtat cgtgcgcatc cacagcggcg ggcagtaccg
     7981 gttcctggaa gagcagggac gtcatcggct tgccgcgacg cacggcctcg tggccagagg
     8041 gcagggattc gaccgtgtcg aacaggcgct catccggggt gtcctcgtgc gtatccgcaa
     8101 ctgcacggcg agaacgacgg cgaaccggac ggtcctcgtc atcatcatcc atatccatag
     8161 acgcacggcg agagcgggag cgaaccgggc ggtcctcgta gtcatcatcg tcggactggc
     8221 gggagcgaga gcggcggcga accgggcggt cctcgtcgta atcgtcatcg tccacaaccg
     8281 aacggcggcg ggaacgtggg gcgtcatcgt catcgaaatc atcggcactg acagtgcggc
     8341 gcgaacgacg aggacgatca tcctcgctat ccgagtcggc cactgcggaa gcggcgcggc
     8401 gcgaacggcg gcgggttgga cgttcggcgg aatcctggga atcgctggat tcatcggaat
     8461 cgctggaatc ggctgacttg gaatcggccg cagtgccggc atcgggaagc gcgggggctt
     8521 cgaacagcgg ggcgcgttnn nnnnnnnnag gcggcggcag gcgcagcgtt cacggctgca
     8581 gccgaatcgg cattggatac ggttgaatta tcggttccgt cgttgacgaa accttcacgg
     8641 ttgtcagtgg gcacaatgct cctcgcggcg cgctgcatca cgacgcctat ttccaggcct
     8701 atacgcttgt ctcacaccac gctctcaccg tggccgcacg aaaacgcgcc gcgtaaaggc
     8761 aacttcgtct caagtctgtc ctgcgggtgg cggacaccgg cgcttgcgtt gtctcattct
     8821 caagagccgc aaggggatcg acgccatctc gcacgctgat ggttatgcag aaccaccatc
     8881 ctttagtatg ccacatattt tgtgcgggcg tggcccggcg aggctgacgt tctctcggct
     8941 cccctccccc gcggtgacag ctcccctcag ggaggggagc tagagaggtg tcagaccagc
     9001 cagtcggtaa ggattgcggc catggcggtc aggtcggatt caggcacctg ctcgtcgtgc
     9061 ttgtgagcca gcagcggatc gccggcaccg aggttgacgg ccgggatacc gagctgagag
     9121 aagcgtgcca cgtcggtcca gccgagcttg gccagcgggt cgcggccagt gcgctcctta
     9181 accagtcgga ccagatcctg ggcgagcggg gcgttcaggc ccgggcgcgc ggaggggctt
     9241 tcgtccttca tttcgatgcc gtagccctcg aacacgccgc cggtagccac atgctcgcca
     9301 ttgccgagtt cggcaccggc gtccgcgccc atcatcagag ccttggcctc ggccaacgac
     9361 ttgtccgggg cgaagcggta gttgacgtgt acgcggcatt catccggaat gacgttcgtg
     9421 cctttgccgc cggagatcag cgtggcgttg aggccctcgc gataatcgag cccgtccacg
     9481 ttcacggtgg ccggctcgta ggcgttgagt cggttcagaa tgtcggccgc cttgtggatg
     9541 gcgttctccc ccatccatgc acgggcggag tgcgcggcca cgccgtgggt tacgacgtcg
     9601 aagcgaatcg taccgttgca accgccctcg atgccgctgt tggtgggctc gccgatgatg
     9661 gcgaaatcgc cggtgatcca atcgggatgg gcctcgacga ctttgcgtag gccattcttt
     9721 tcggcgacga cttcctcatg gtcgtagaag acgtaggtga ggtcgacttt cggggtggtt
     9781 tccggcgtgc ggccgtcgag cgtggcggcg aggtagagca tcaccgcgtc ggaggccttc
     9841 atatcggtgg caccgcggcc ccacagcacg cggtcttcgg gatgagcgtg ggcgatttct
     9901 tcgcgaatca gcgaatcgcc gggctccagc cactttggtg ggaaattgtc gatgaccggc
     9961 acagtatcga ggtgcccggc caagatcaca cggctcggct tgccgaagtc ggtgctggcc
    10021 accactgtgt cgccgtgacg gcggacggtc aggtgatctt gctcg
//
LOCUS       DAWBMW010000048         9813 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_1686_length_9813_cov_2.441279, whole genome shotgun sequence.
ACCESSION   DAWBMW010000048 DAWBMW010000000
VERSION     DAWBMW010000048.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 9813)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9813)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9813
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1686_length_9813_cov_2.441279"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1220..1229
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3425..3434
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6973..7080
                     /estimated_length=108
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7330..7359
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1890 a         3041 c         2874 g         1850 t
ORIGIN      
        1 ccccggtgcc gctgggcgac tacatgtccg gctccaacca cgtgctgccc accggcggca
       61 ccgcccgctt cgccgcaggc ctcggcgtgc acaccttcat gaagccggtc gaagtcatcg
      121 aatacgacga ggaaggcctc aaggccctcg ccgcccgcat caacgccttc gcggtgtccg
      181 aggacctgcc cgcccacggc gagtgcgtgc tctcccgctt cgtcaaggat ccgtatgaca
      241 aggccaccct gcgcgagcag gagaaggaag ccggcctgcg atgagctcga ttcctgcaaa
      301 cctgccgctg cgcaacgacc tgatcggtga ggagccgtac ggcgctcccc agctggacgt
      361 gccggtgtgc ctgaacgtca acgagaaccc gtatgcgccg gacccggccg tatgcgacac
      421 catcgccaag cgtgtgcgtg aaatcgcccc cacactcaac cgatacccgg accgtgagca
      481 catcgaattg cgtcaggcat tctccgatta cctggcccgc gaatccggta cccgtctgga
      541 cgtggacgag ctgtggggtg ccaacggctc caacgagatc attctccagc tgttccaggc
      601 tttcggtggc ccgggccgca ccgcactcgg tgccgacccg acgtactcca tgtacccgga
      661 gtacgcccgc gacaccttca ccggttggaa gctcgcccac cgcaacgccg acttcacgct
      721 gaacgtcgac aaggtgcttg aggccatcgc cgaggtcaag ccctcgatgg tgctgctcac
      781 tagcccaaac aacccgaccg gcaccccgtt gccgatggag gatatcgaac gtattctcgc
      841 cgcctgcgaa accgctgaag tggtcggcgc gggcgagggc gtgcatccga ttctcgtcat
      901 cgacgaagcg tacgtggaat tccgcaagcc cggcaccccg agcgccgtga gcctgatcaa
      961 ggaccacccg aacttggccg tcagccgcac catgagcaag gccttcgcct tcgccggtgc
     1021 gcgagtcggc tacctggccg cgtccaaggg catcatcgac tgcgtgcgta tcgtacgtat
     1081 gccgtaccac ctgtccgccg tgactcaggc cgccgcactg gccgccttcg aacacaccga
     1141 cgagcagctg agccgtgtcg agcacctgcg cgaaacccgc gaggccaccg ccgcatggct
     1201 caaggaacag acctacaagn nnnnnnnnnc cagccgcttg aagtggccga atccggctcg
     1261 aacttcctgc tgttcggcgg gcacttcgac aagcgtgaag ccatcttcga cgagctcctg
     1321 aagcgcggcg tactcatccg cgtggtcgga ccggacggtt ggctgcgcgt gtgcatgggc
     1381 accgacgagg agatggaaac cttccgtaac gcccttgttg aagtgcttcg tatagtggaa
     1441 gcagcataag ggacgtttct ttccgattgc ggcgagaccc gaagcgattc gagtcccgcc
     1501 gcaatccaat atgacgaatc acacaggaga catgatggca agaaccgcgc acatcgtccg
     1561 cgaaaccagc gaatcacaca tcgacctcga actgaacctc gacggcaccg gcaaaaccga
     1621 tatcgataca tccgtgccgt tctacaacca tatgatgacc gcgctcggca agcattcgct
     1681 gatcgacctg accatccacg cccacggtga caccgacatc gacgtgcacc acactgtgga
     1741 agatacggcc atcgtgttcg gcgaagctct gaagcaggct ctcggcgaca agaaaggcat
     1801 ccgccgcttc gccgacgcca ccgtgccgct cgacgaggcg ctggccaagg ccgtggtcga
     1861 catttccggc cgcccgtact gcgtgtgcag cggcgagccc gaaggctacg aatactgcat
     1921 gatcggcggc catttcaccg gctcgctggt ccgccacgtc atggaatccg tcgccttcca
     1981 cgccggcatc tgcctgcaca tgcaggtgct cgccggccgc gacccgcacc acatcgctga
     2041 agccgaattc aaggcgctgg cccgtgcgct gcgcttcgcc gtggaaatcg acccgcgtgt
     2101 agacggcgtg cccagcacca agggagccct gtgatgaccg acgacaagtt cgatccggac
     2161 gaatcgcact tctccgacga ggagcttgag gctgcgcccg ccggattcga gaaagaattc
     2221 gcggacgatt cggtatccgg cgatgccagt gataccagtg atgccgataa aacagcggac
     2281 gctgcttcag cgaagtccga ttccgacgcg aatgatgata cggctgataa ggcttccgct
     2341 gattccggcc agcctatgac cgacgacgtc accgggtccg ttgcggatgc ggtgaacgag
     2401 gccatggccg acgtggtcga cccctcgctc ggcttcgaca acgaactcgc tggactgttg
     2461 ggtaacaagg cgaaggtggc gctgatcgtt acccgtcttg cctccgccga actgttggcc
     2521 gcgttctgcc agctgtcgga catctccgcc gcatgcatcg gtgccaatca gggggccgtc
     2581 gcggtgctga agaatctgaa cggcgacggc ccggaagccg cagccaagga cctgaccacc
     2641 gtggtatccg gcatggccgt gattcttgca gtcaaccgtg ccgacaagct tgaagtggcc
     2701 atgtacgtgc agggagaggc cggccagagt ttcgcgccgc ccgtgctgtt cacctccacg
     2761 ccgcgcttcg tcgaggacct gatgctcggc atcgtaaccc tgaatcaact caagacccaa
     2821 gggttcgaag tggtggattc cgctggactc gaccatgatc aggcgatgca gattctcgcc
     2881 aaccacacta ggcgcggacg tggcgggcgc ggctcccgca tcgaatagac gccaaccaaa
     2941 ccagtagggg ataggagagc agatgaccac agcggttgtt ttcgattacg gtttcggcaa
     3001 tgtacgttcc atggtgcgtg cgctcgccaa tcttggcgtc gacaccaccc tcacctccga
     3061 ctaccggcag tcgttggagg ccgacggcct agtcgtgccg ggcgtgggtg cgttcgccgc
     3121 atgtatggaa ggactgaaga aggtcgacgg cgaccgtgtg atctatgatc gcatccgcgc
     3181 tggccgcccg gtgctcggcg tgtgcgtggg cgagcagatc atgttcgaac acggtctgga
     3241 gcacggtgcc catgccgcag gcatcggact gatcggcggt tcggtgaacc tgctggacgc
     3301 cgacgtggtg cctcacatgg gatgggacac gatcgaggcc gcccccgatt cggtgctgct
     3361 caacggcgtg gagaacgaac ggttctactt cgtgcactcc tacgccgcga tggaagtgaa
     3421 gccgnnnnnn nnnncgtaag gccgatcgcg ctttgtggcc gcctacgaac acggcccgct
     3481 gtccgtcacg caattccacc cggaaaagtc cggggacgct ggctcccagc tcctcaagaa
     3541 ctggatcgcc acgctgtaaa aaagcatgaa gctccctctg acgagggagc tcaaattgtg
     3601 tataagtaac tgaagaaagg ctcttcttat gtcgttgact ctgatgcccg ccgtcgatgt
     3661 acgcgatggc aaggccgtgc gcctgcgcca aggcgaatcc ggttccgaaa ccgattacgg
     3721 ttcgccgttc gaggccgccc gtacttgggt tgaggccggt gccgaatgga ttcacctggt
     3781 cgaccttgat gccgctttcg gtacgggcaa caatcgcgat cagctgcgag aaatcgttca
     3841 tgagctcggc gaccgcgtga acatcgaact gtccggcggc gtgcgcgatg acgcttcgtt
     3901 ggacgcggcc cttgaggccg gtgccgcccg agtgaacatc ggcaccgccg ctttggagaa
     3961 tccggattgg accgcctcgg tcatcaagaa gtacggcgac cgcgtggctg ttggtctgga
     4021 tgtgcgcggt cacacactgg cggcgcgcgg ctggacgcgc gaaggcggcg acctgtttga
     4081 gaccatgaag ttccttgact ccgtgggctg ctcgcgttac gtggtaaccg acgtggctaa
     4141 ggacggcatg atgtccggcc cgaacattca attgctgagt gaagtcgctg aacgcaccga
     4201 cgccaaggtc acggcttccg gtggtatctc caagctggac gatttgcgcg ccatcaagga
     4261 actggctgaa attggtgttg attctgccat cctcggcaag tccctctatg cacgtgcgtt
     4321 cacgctccag gaagcgcttg aagtagcgaa gtaggcaaag gactcgcctt agcgagactg
     4381 ttaatctacc ttcgctcagc tatcgcttca ctcagacccc tcctacggac agcccaatgg
     4441 gctgtccgct taacggctca gcaagggagg tcgccggatt tgaggagctt tttgaagacg
     4501 gcgtagtctt catcattctg ttcggcgtag ctgacggcga atgaggcgat ggcctgatcg
     4561 aattcgtcgg tgccacccat gtaattggcg atcgcaatcg aatcgccggt gcgggcgtgg
     4621 gcgtgcgcca acgaccatgc gcacatgcgt gacagatcgc tcaatcccga agcgttgaga
     4681 ttgtcgatat caatcgaccc cttgccattc cacaactggc gcacgtaata gtcgcgcggt
     4741 cggccatcct cggccataaa gctcgaccag cccaacagca catcggccgt attctgaatc
     4801 agcttctgcc cctgaaccac gcgctcaccg tgcgtggcgt acggcgacgc gccgcagtaa
     4861 tgctcgagca cggaatacgt ggcctccttc atctgaatca tcatcggatc gtcgatatca
     4921 cgcgcgatcc acaccgacgt ccacgcgcgc tggcccaccg agcccacgcc caccaccttg
     4981 cgggccgcat cctgatagcg caaatgatcg aacacccaac ggcgatcgtc gtacagggaa
     5041 tgccggtagc tctcgaacag cgtgtctata cgtgcctgca acgcatccaa gtcggcgtaa
     5101 tcatccagct gattgatcgg caccagctcg ggcggattgg atttgaacct gagcttgccg
     5161 ctctcataac ggcacagttt ggccgccgcc gcgtcgttat ccttcagcag cgccttcatg
     5221 gccgcctcac gcagcacacg gctgcgtttg gaaccggtgg tctcgaagcg gtcgagcgtc
     5281 tcctccacgt ccaggtgctc ataccacgcg tcaaggtaat ccatttcgga gaaccacttc
     5341 atgcgtgtgc ggtacgtgtg tacgcactgg accacggcgg cgcgacggtc cttctccttg
     5401 attttgttgg cgcgtccgca gatctccacc gaggcggcga gacgtttcag atcccattcc
     5461 cacgggccga tagcggtttc gtcgaaatca ttgatatcga acaccagacg ggcggaaggg
     5521 gagcggaaaa tgccgaaatt gccgatatgc gcgtcgccca cgcactgcac cggaatgcca
     5581 gtgactggcg tggaagccag gtcattggtc atgatgagca cggtgccccg gtagaaggtg
     5641 aaggggctgg cgctcatacg cttgtaacgc agcggaatca gatccggtac gcgaatcgcg
     5701 gactgctcct ccaaaaggct gatcgcctca cgccggccct ccttgacctt ccagatggca
     5761 tgtgcgtcca gcggctcctt ggtacgagtg gcgatgccgg cgcgcgaacg gtcgcgcggg
     5821 tcggtggcct gcgtccagct ggtcacgtcg acgggcggat tggcggatgg ggtgagatga
     5881 tcttcgctca tagtcactac catagtgccg tgccggcatg ggcctgaacc atgcctgaca
     5941 tgcggaccgg ggcatgttga tgaaatctcg cgactcgcag gtgacgaaca gtgagatgtt
     6001 aacgcccttc gtaacacggt tatggaacga taggcgacta tggataaaca gcaggagttt
     6061 gcgctgcgca cggtcgaaga acgcgacgtg cgctttattc gtctgtggtt cacggacgtg
     6121 ttgggaacac tgaagtccgt ggcgattgct ccggccgaac tggaagccgc gtttgaggag
     6181 ggtctcggct tcgacggttc cgccatcgag ggcatgaccc gcgtgtccga agacgatatg
     6241 atcgttcaac ccgatccctc gacgttccag atcctgccgt ggcgcggtgg cccgcaaggt
     6301 accgcccgca tgttctgcga cattctgacc ccggacggcg agccttcgct cggcgatcca
     6361 cgtcacgtgt tgaagcgcac gctcgccaaa gccaaggaca agggcttcac gttctacgtg
     6421 catccggaaa tcgagttcta cctgttcgaa agccaggacg actggtccaa ggccccgacc
     6481 ccgatcgacg agggcggcta cttcgaccac gtgccgcgca gcccaggcat ggacttccgc
     6541 cgtgccaccg tgaacatgct cgagcaaatg ggcatctcgg tggaatactc gcaccatgag
     6601 gcggggccag gccagaacga gatcgatctg cgatacgcgg acgcgctcac catggccgac
     6661 aacatcatga cattccgtac ggtggtcaag gagatttcct tggagcgtgg cattcacgcc
     6721 agcttcatgc ccaagcccct ggccgacgcg cccggttccg gcatgcacac gcacctgagc
     6781 ctgttcgaag gcgattccaa cgccttctac gaggccggtc aggagttcaa catgtccctg
     6841 accgcccgcc agttcgccgc cggcatcctg taccatgcgg ccgaaatctg cgcggtcacc
     6901 gaccagtacg tcaactcgta caagcgcctg tggggcggta acgaggcacc aagctacatc
     6961 tgctggggcc acnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7081 tcaggctatg ggcattcagc cgctgccgcg ctcgctggac gaggcgctga agatcatgga
     7141 gaaatccgac ttcgtggccg acgtgctcgg cgaacacgcc ttcggctact tcctggacaa
     7201 caagcaccag gaatgggagg agtacaacca gcaggtcacg ccttacgagc tgaagaagta
     7261 tctgccgaag ctgtaatcgc aagcgttgca catgtgccaa accccgtcga tgattggtca
     7321 tcggcggggn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt cgtggtggtg ggtagcgggc
     7381 agaggcggga aaagtgttat cgctgagttg atacgaaaaa acgattgacg taaccgacga
     7441 atcagcttgt agtacgagcg agagcactca agagagcacc catgacgaac gcacctgcga
     7501 acaccgcgcg taccggccgg cacttgggtg gccgatacct ttggttggcg tgtcaccttt
     7561 gccttggtct cggtgctcac cgccgtgctg atggcatcga tggcagtgtc tctgccacgc
     7621 aactcgcatc cggcgaagat cggtttcctg aggcaattcc gcctgttctt cgaccgccgc
     7681 atccagctcg gcgtggcgga tgtggtcttc ggcgcagccg cctcctatgt cttctacacg
     7741 tatctttcgc cgatcatgcg cgacgaaatc ggcgtgcccg aacgctatac cagcatcggc
     7801 ttggtgattt acggctgcgc ctgcctgtgg agcaacctgt acggcggcaa gctggccgac
     7861 aaaggtcatg gcgtggaacc attgacccac attcgaccga tctactgcgt gcaggccgtc
     7921 tgcctgtgtc tgctggcttt cgcctcgctg ctggcggttg gcgccacgac cctgctgcgt
     7981 ccatacgtcg aacgctgaca gtgttggccg agtccaatcg gacaagaccg gtcagctgcc
     8041 gttatcagct tcgcggcgaa ccgaaccagc tggtgtagat aagcgcgaag ttgcggttgc
     8101 cataactgga gcaggaatcg ccggtaccgt tgccagcggc cagtgcggcg atgttcggct
     8161 ggtagggcgt atagatgtag agcagggcgg tcgccttgtt ctcgatatac acttgtgcgc
     8221 cgccgcatgc cgcgttcgga tgatattgga tgtagttgag attgtgcgcc gtatacccgt
     8281 actgttcctc gtgctcgaga tagtaacggt accgcttggc cgcgccatac acctgccgaa
     8341 agaatccggc ataggcggga tcgcagtcgg cggtatccgg acagttcaac cccatcgccg
     8401 ccataagctg atagtcgctg ggatcgacgg cagtgaccag atgctgctcc ttctgcatca
     8461 cggtgagcag cactttctgg ctcaccttgc aggcgcgggc cgactggtcg atgattgccg
     8521 ccgccgactc gcctttggtg cctgtatagt ccgcgcacag gccttcgcct gactcgttcg
     8581 atgtgtcgaa ggtcatcgcg gccagcgagc caccttgtgt gtccaaaaac gattggactt
     8641 cggcctgact catcgccgaa ccgttgaaga actgaccatc cgaaataata ttgcccggat
     8701 tgaaatcgta tgttcgcgtc agttccagct gtgccgcctc ggctgcgcga acctgtgccc
     8761 gccagtttgt gaaacgaacc agcccggcca ccagcgctac gacaacgacc accgccaccg
     8821 tgggaatcag caccatcttg atacgggtcg gccagttcgc cttacgccag gaacgcttca
     8881 gagcattgat cggaccgtgc ctacgattgc gtttcctgtt ccgattgccg aggcatccgc
     8941 gcacttgccc acgctgtgat gacggcttgc gcgtgcgctg gcgcgtggcc tggcgacttg
     9001 tttgccgtcc ggtcacgcca gcgctttctt gatacggttg agcgaagccg gccccttggt
     9061 gccaagctcc tgcgcccata gggacacgta gaactcttca agcatccaac gggcctgttc
     9121 ggcctgcttc atcaatgcct catgcttcgg gccggccggc tccgccttgg cacgggccaa
     9181 cgccttatca accacctgac gggcctcgtc ggcctgccaa gcccacttga catcacggtt
     9241 cttatcggtt ttcgccttat tgagtcggct cagatcggct ctcaaatatc ggctcaacga
     9301 cttcagcgca tccgggggag tgcgcccgat gaatccgggg tagacgagcg tggcgatatg
     9361 gtcgcgaatc gactgcaaca ccgaaagcat cggcagatca gccttgccgc cagtggcctt
     9421 gtcgacctcg gcataagcct tgagcacggc gatcacgtca tgcgccacct gatagacggt
     9481 gtcctcgtac acttcgcgca catcgagcac cgcattggct aacgcctcat cgtccgagag
     9541 cctgtcgaca ttcggcatca gccgtttgac cgtggccagc tgcaggtctt cgaccagatc
     9601 cttggtggac ttgtacggag cagaagccag catcaacgcc tcggtaccca gccagcgcga
     9661 cgaaatgcgt tcggccggca atcgcaacgc atccaatgcg ccacgccaga gcatcgtggc
     9721 ccgggactcg gtggtggcac ccgccttatg caacaggttc gcctgactga ccagcttgcc
     9781 ctgttctcca gcctgatcgg cctgcttctt gac
//
LOCUS       DAWBMW010000049         9626 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_1726_length_9626_cov_2.482499, whole genome shotgun sequence.
ACCESSION   DAWBMW010000049 DAWBMW010000000
VERSION     DAWBMW010000049.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 9626)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9626)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9626
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1726_length_9626_cov_2.482499"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4198..4207
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7255..7264
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2013 a         2959 c         2775 g         1859 t
ORIGIN      
        1 atccatgaca gcccggtgct ggtgtctgcc ttggcgaaca gataagcggt gaccgcatcg
       61 ccggcagtga tggacggcgc caccgatgca ttggatgcgg tcggcagcgg cagcgcgaaa
      121 tcagaaccct tcggtgtcag accatccatg gtcaccgtgc gcaccacgga cgcgatagga
      181 gtctccttgc tggaaacggt cacgaacaga ccatgctgcc catcgaccgc ggcggacagt
      241 tccatcgagg actccccctc ggctggcaca gattcgagac gatgagctgc tgtgtggtac
      301 cggtcgtgct gtcggagagc aggaacgact ggctgagcga ggtcgatacg caggatgccg
      361 ccgatatgcc tctcacatcg ccatcatcgg cccatgatgc gatggtgccg acggtgccgg
      421 tgccagctgc ggcctgcagc atacgggtat tgatcagacg agctccctga ttgatcttct
      481 gagcaccggt tttcaccgag gaatcgtcgg tcgaatcggc gtctttcaaa gttacgtcat
      541 ctgaactgga cttttgcccg atcgccgaga cagtggcgct gtagacggag ccgaacgccg
      601 catagcgcgc ttgtgtgctc agattgccga cagtggcctg gaaggcgctg tcgccgtacg
      661 tgccggtatc ggccaatcct ataggtgccg ggcagtaggt ttcggactgg gtcggactaa
      721 cggtctggtc gatggcatta tcggaagcgg ccaccttatc cacccagata ttggggatgg
      781 gggccatgac gagaacgacg aacagcgcaa tcaacaccag catggtgatc acaccgagca
      841 cgatgccgat cgcacgtttg aaaccagaag ttttgttgct catgcctgct cctccaatcc
      901 gtaggacggt cgtctgcgag gcatggcgac caagcagtac aacgccgtga tcacacctag
      961 gcatatcagc caggcgtgcc tccagacact atgctgtgca cgttgctgcc atgaggtatc
     1021 cacctgctgt gaggcgctgc tacgcagcgt cagacgatag tagctgccgc tatccgtgga
     1081 gaccagggac tgggtgccgt tcgatgcggc gatattcgcg ctcaactgtt catacggcat
     1141 gtcgttcaag gaatcgccgg tctcgggaac cacgtagatg ccgccgaatc caagcgcgct
     1201 gatggccgcg atggcctctt cgtcgggatt ggacagcagc gaggcacagg cctgggccag
     1261 ttgttcgtca accgcatcat gcttgccgga cagcaggcgc gcccgctgga ccggagagct
     1321 gtcgatcaaa tcgccgcgcg aggtgcgcat caccgcatag ttgacaatgt tgcgggtctc
     1381 cgcgctcacg gcaagcacac gatgatcggc accggcctcc agataatcgg tggtgaccat
     1441 gggcaggccg ttttcagaca cgcccagtcc cgcatcctta tgacgttcga tgccatacat
     1501 gcattggatg ccaatgcagc acaccagtat cagagcaagc accacacggc cagacacgat
     1561 gagcacatgc agacggtcct ttttcgatgc gggatcactg ccagacgcat gcaacggttg
     1621 gaatcgcttc accgccccac cagcgacgag acacacgcag gcgaggaagc ccaatatcat
     1681 catcgcgatg cccggctgcg cggaaccggc cacgacacca tcggcatcca cggttatggc
     1741 cacacggctg gagaccaggg cgagcgcgcc tccgcagacg attaccaccc acatcagtcg
     1801 cgaggtccgc aacgcgaacg gcagcaccag cgaagcaaga gcaagcagcg tgatgacgcc
     1861 aaacagcaca gtgaccgcca aatcggcata atcagtcgac cccattcgcc atcccagcgc
     1921 acgctgtgcg gcctcaagca gactcagcga cgccggcgac ccgttggcgg atgacgttgg
     1981 caccgtgatg tcgccgaaca gttgacgcca catgcccaaa tcggcgtaac gcaccgcatt
     2041 gacgagcgta ggagccaccg caaaggcggc aggaaccgga attagcaaca gcatggcgcg
     2101 cttgcgacgc acgaatagca ggaacatcag gaacacgacg atcagcgcga acagcagctg
     2161 cggttcagcg gccaccacag ggatgaagca cagtgcggcg atggccgcgg cctgaacgga
     2221 tgtgcgcggc ttgagcgggt cttcggtatg gtacatgccc acggcacgga acacgaatgc
     2281 gaacgcggcc ggcaggaaca ccattacggt aagcatcggc atattggcct gcgcatacca
     2341 gccgaacata atgccggtgg atgcccacag cagtcctccc agcacacgaa ccacgtccga
     2401 gcgggtgaat acgcccgcca gcgcccagaa cgacaatgcg ctcagcggcg cggccacaaa
     2461 cagtatcatc gccaaagcgg cggtcacatg gccgagcgtg accaacgagg ccagcatcag
     2521 caccagcagc cacggcgtgg gcggcgcagg aatgccggtg ccgctgccaa acacccacgg
     2581 cgtggtggcg gactgcacca gttgcgtgaa gctggcaccg gtgggaagca acacatcgga
     2641 atacaacgat ccgccggaga atatcgaacg gaacaccggc caatacatca ccgcgatggc
     2701 cgcaaagcac accagcgcca tcaccacggc cgcactccag cgatatatgg tgcgtgtccg
     2761 cagatgggca cgttccagcg gactgagcag cactctgccc tgctgggatt ggaatgcatc
     2821 ccgacgatca tggaactgct tgatctgctg gctgtctgcg aacaagacat gcagggacga
     2881 tgcggcgacc ttcgattgtc tggccaccag actgcgtgat ttcatgtttc ccgcgatatc
     2941 ggccaaggcc agccatggca ggcacaattg cacccatgct tcatacggct tcttgccgaa
     3001 catcatggat atggccatgc caaaactgcg gagcagcctc cacagccaga cgataaacca
     3061 ggacgacatg gccaggtccg tgtacagata ccgctgttgg gagcgatgca ccgtcatcgc
     3121 atgattgacc gtgtgatcgt ccttaagcgg ttccccgcct cgcgtacgaa tgccctcata
     3181 ccgtgcgcga cgatgtgata tctccgcttg cgggacaacc accacgcgtc cgccgctcag
     3241 gcagacacgg cggcagaaat caaccgattc gctgtaggtg gtcagccagg cattgatgcc
     3301 ccgcagtcgc gtgaattgcg acatggacac cagcgcgcct gccagcgaca ccgcaaacac
     3361 atcctgccgc ccgtcatact gctcctgatc gggttctcca tcgaccacca gggaatgcac
     3421 ggcgtggtga cccgcataca taccgacatc atgcagatgg gagccttccc aatcgcactg
     3481 tttgcacccg agcacactgg cgcccggcgt attgcgccac gcctcaagca atcgttcaag
     3541 gcatgagtca tccgaagggc gggaatcatc atggagcagc cacaatgcgc ggggagcgtc
     3601 atccagatcg gcacgatcca atgcccggct gaccgcatcg ccgaaggagc gtgcgccctt
     3661 ggccgagacg atgtgaatgg tcacatgctt ggactgcggc acctccatca ccggccccga
     3721 tggcgagggg atgacctcga acgaggtggt tatcctcgaa ctggtgcgcc cggtcgcgtc
     3781 ggcgatgatg acgacccccg gaagaacatt ctgcgtcaat accgaacgca atgtagaccg
     3841 gaggaaacgc atatcctcct caacggtgat cacagccgta atgccggtat ccacatcctg
     3901 cctatgcgta tatggtcgag aagacatggc gtcggccaac gcctcatgaa tatcgctatt
     3961 acctgtcgga ctcataaact cccagtgtac gcaccagact tgaagggggt gtgtggagct
     4021 acgcccgctc ccggtgctct cgcttatatc gacggcgttc gcgctcgctc atgccacccc
     4081 aaataccgaa tcgctcatca tggtcgatgg cccatttcag gcattgctca cgcacttcgc
     4141 attttgcgca cacacgtttg gcatcacggg tcgagccccc cttttccggg aagaacgnnn
     4201 nnnnnnncgc acaacgcctt gtgctgccaa gatacatcgc catcaggtcg gaatagtccc
     4261 cacaattcgt gccatggctc ttcggctgaa tcgtcgacca caccccacat gcgattctcc
     4321 ttgcctattg agcttttcat acggactaaa ttacacacat gcaatttcgg ttttgtcaaa
     4381 ttctttctcg tgtcattaca tcatttttat ggaactgtgt gctatggcgt gaacgcgatg
     4441 ccctgacgcc gttgcacatg atattgtgcc gttcctgcgc aggaacacca tagcgcagga
     4501 acacccggcg gatgctgtgt caaagtgtca ttcagacaat cgacagtttc aggaggggtt
     4561 atgacgcagg aatcaggtgg ggcggatccg catatcaacc cgccaagctt cattcctgcg
     4621 ggaaatcgac gcacccggcc ttccgggcag gtgccacgct ccttcgcccc cgcccgaacc
     4681 tcgcgccagt caggtcaagc tccatcggcc cgtccgtccg tcacgggaaa tgacatcgaa
     4741 gagcctcaac tgaacgccaa cgccacggca agcgccaacg tcgcggacaa cggcaggcgg
     4801 actccgccca gtttttctcc gaaaaccgga cgcgccactc gcagcacggc tccggatgcc
     4861 gccgcaccgc gcagcattcc gccgtcgcga gcgcggatgt cttccggctc atcccgcaac
     4921 gccgtgcgca atccgtcgga tatgccggtt tcccggccct catcgccagc agcaaacgtg
     4981 tccacgaagt tcagccatct tggccggccg gcggcggcaa cggcgaaaac ggtaacggca
     5041 cgccgtccgc gagccttacg catcacccta tgcgcaatcg cggtactggt ggcggcgttg
     5101 attctttccg ttttcggtgc ctggcaatgg gtggacagca aactgaatcg gagcgactgg
     5161 ctgaccgaca cccccaacac gccggcctcg acttggctga ttcttggctc cgatgagcgt
     5221 gacggttcca ccgattacgg cggcgtagac aatatctctg gcttcaggac cgatacgatt
     5281 ctggtgctga tcaagcccaa aagcgggccg agttcgttgg tttccattcc ccgcgattcg
     5341 ctcatgaacg tcgataacca gtacatgaaa atcaatgcgg tggcgctgat tgcgggcaag
     5401 aaggctttgg taagcaacgt cgagcagatc accggccaga aaatcgatca tgtggcacag
     5461 atcaagttcg gcggtctgca gaacgtggtg aacgccttgg gcggtatcga actgtgctat
     5521 gacgaggatg ttcaggattc ctattccgga ctgaactgga aggcgggatg ccatacggcg
     5581 gatggttcca ccgcattgtc cttctctcgt atgcgatatg ccgacgcgcg aggagacttc
     5641 ggacgcaacg aacgccagcg acaggtcatc tcggccatcg tcaagaaggc ttccagcagt
     5701 gagaccctga ccaatccgtc caaaatcgtg gcagtgtcca atgccgcgat gagcgcactc
     5761 accgtggacg agaagaccaa cccttacacg ttgctcacca tggcgttggc gttcaaaagc
     5821 gccaccggct cgaatggcat ctccggcagc gtgtactggt ccgaccccga ttattacgtt
     5881 gatggcgttg gttcctcggt gctgcttgac gacgccaaga acaccgaact gttcaatcaa
     5941 cttgccgacg gcacgtataa ggccggcacc catcggcttc atcatcggcg catgatcgtc
     6001 accgttgcct acaccgccaa tcatgcacgg gcaaaatgtc atgtggataa ctcggcaagc
     6061 tagaaccaca taccttgatt cggcttgcgc tttcggcatg cgttccccca cgctggaagc
     6121 atgacatcac agcagaacga ttcgaagcga cattccgtcc atacggcatc cgccgtatcc
     6181 cgggcgaagc accgtttggg cgcgccgcca ttgcgcgccc gcctgaccat gtttgccgcg
     6241 gcatgcccga tgatcgcgca ggcattgatg atggtcatac tgctgttgca cgggcaatgg
     6301 ctgttcgccg ccatgatcgc accgggcata ttcgcctgtc tcgtgtccct gctgctgacg
     6361 ctcccctcgc ctccaggccc ggagaaggct cccgatccgc aacaggcaac gatcgatgtc
     6421 ggtatcacgg gcgacgccga tgaccgcgcc gcagacctcc gtcatgcgcc gtcgcagccg
     6481 atagaatcgc tgctgcactt cgcacgcctg ccatggcgcg ccattgtggg ccgctggctt
     6541 gaaccccttg accttgcggt gccgatcggc atgaccggaa gcgagccgct gatgctcgat
     6601 ctgaaccgtc aggggccgca tgctctggtg gccggcacca ctggttccgg aaaatccgtg
     6661 ttgctgcaaa gctggtgtct ggcgctcgcc tcgatgaacg ggcctgagca tctgaatttc
     6721 gtgttcttgg atttcaaagg cggttccgcg ttccgcaaac tcgaacggtt gccgcatacc
     6781 gtgggcagcg tatgcgatct ggatctggcg catgccgtac gcgcgttgcg tgcgctggaa
     6841 gccgaactga cacgccgtga acagctgtcc gccgctgtcc atgcttccga tatccgcgat
     6901 atggtcaatc caccaccgcg cctgatcgtg gtcattgacg aattccatgc cctgaaggac
     6961 cagctcccgg attatgtgaa tcgtctggtg cgtattgcct cgctcggcag gtcactgggc
     7021 atgtatctga tcgcctgtac gcagaatccc atgggtcagg tcagtgccga tatgaaggcg
     7081 aatatgtccg tcagcatttg tttacgggtt cgggaccgtc tgcaatcatg cgagctgctt
     7141 ggcgatggtc gtgccgccga cctcagtccg gcaatgcccg gtgcggcgtt ctgcaacgat
     7201 tccgaacagg tgacggcatt ccgctgtgca acggcacgcg atatcgatgc ggtgnnnnnn
     7261 nnnncgttcg catcgcggtt cgtcggaagc ccgccgcaac cgtccctgtt taccgcgcca
     7321 ctcccccggc acgtaaaaga ccggactgtg gccgaccacg caccgcaacg cattcgattt
     7381 ggacttgccg acgatggcat caacctgcga gaggcgacgg tatccctcac cggtggcaat
     7441 atcggtgtca tcggcccgca ggggcgcggc aaaaccacgt tgctgaaaac attggctcga
     7501 cacgccagca tggctgatgg cttggcggta cgtgtcagtt ccccgcatcg tcgggtatgg
     7561 agctcgcaat ggcttcatgg cggccgctgc acgccgtatg ccagctcgga tgcaccaccg
     7621 cctccgcata tcgtgtggtt cgtcgacgac gccgatgagt tgttcgaccc attccgcacc
     7681 gacgagcagg cgctgtcttt cacacatgcg ttggccgatg agtccgtcag cgtggtattc
     7741 gccgtcagca cgattcgccc gatacgtata ccggagcatt gcaataccag aatcgtgttc
     7801 ccgtgcgggg agagaacgtc cgatcttatg gccggagtcc ccgctcgcct attggatatg
     7861 atgagtcata tcgatgccga caacgcgggc cgggccgtgc tcatcgaggg cacatcggca
     7921 tgtctggtgc agtgcgcttc gtgaaaactt cggttttttc tcggcaaaac ctcttgaaaa
     7981 tcaccacggt tcgtggttac cttggtactg gacttaagga acaacaccag ccacggggct
     8041 tttaagggag gcaagcaatg agcagcgctt ttgattggcg agccaaggcc gcatgtcgtg
     8101 acaaggatcc ggaactcttc ttcccggtgg gcaacaccgg tgcagcctat cagcagattg
     8161 aggaggccaa ggccgtgtgc cgcacctgca aagtgattga cgcatgcctg aagtgcgcac
     8221 tggacaccaa tcaggattac ggcgtgtggg gcggcttgag cgaagatgag cgtcgtgcgc
     8281 tgaagcgccg cgccatgcgc gcccgccgtt ctcaggccat gcagatgcaa atctgatgtt
     8341 ttgattatcg cttcgtcata ctaggaaacc ctcccgcttg ggagggtttc ctcatttcgg
     8401 gcttcgcatc actcgtcctg agcagccctg agcttcatat caagcaccac gcgggtgccg
     8461 ccttcgcggc gcggctccca atgcacggaa ccaccgaaat cattggtcac aaaggtgttg
     8521 atgatctgcg tgcccaaacc ggaaccggaa gaacgagcca atccaccctg ttcttcggag
     8581 ccgagaccgg aaccgtcatc ctcgaccacc acattgaggt tcgggccgga gcggcccacg
     8641 gaaatcgtga tatggccatc cttgcggcct tcgaacccat gctccaccga attggtgatg
     8701 agctcggtca gcacgaggga cagcggcgtg gcatcctgag cgggcatcat gccgaacttg
     8761 cccatgtagt tgatatcgat gtgctggtct ttcatcgtgg ctaggtccac ggccatgcgc
     8821 agcaggttgg cgatcacctt gtcgaagtcg acgacctcgt cggcggtctg gctcaatccc
     8881 tcatgcacca tggcaatggt ctgcacgcgg cgttgcgcct cctgcaattc cttcttgacc
     8941 tcatccgact tggtcttacg agcctgaagc ctcagcaatg cggagacggc ctgcaggttg
     9001 ttcttgacac gatggtgaat ctcggaaatc gtagcgtcct tggtctgcag ctcctgctca
     9061 cgacggcgca gctcggtgac gtcacggcat agcagaatcg ctccgatacg gccgttgttg
     9121 tcatacaagg gcaacgagcg catcgagacg gcggaacggt tggcgttgag ttcggagtcg
     9181 accgcggctt tgcctgtcaa caccaacggc agggtttccg gaacagggtc gttctccttc
     9241 aatagctgtg tgccgagttc gctgaggtaa tggccaggca tggtggtcag cagaccgaga
     9301 cgcctaaagc agctgatggc gttcggggct gcgtatcgaa ccacgccgtc catggtcagg
     9361 atgatgaagc cgtccgccac gcgagcgata tggcgttggc tcatcaccga atccttgtag
     9421 gggaattggc ctcgcggaat catctcgtac agatgcttgc cggcgttgat gctttccgac
     9481 tcatatcggc cgttggattc tcgtgtggcc atattggtct cacgcacgac caagcccaag
     9541 gtcttgccat tgtgccgaac cggcgcgtac acgttgcaga cggtcgcctt gcccacggtt
     9601 ctcaataccg tggagcggaa caccac
//
LOCUS       DAWBMW010000050         9598 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_1734_length_9598_cov_1.903385, whole genome shotgun sequence.
ACCESSION   DAWBMW010000050 DAWBMW010000000
VERSION     DAWBMW010000050.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 9598)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9598)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9598
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1734_length_9598_cov_1.903385"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1851..1860
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4128..4137
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4408..4417
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7510..7519
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9092..9101
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1862 a         3006 c         2864 g         1816 t
ORIGIN      
        1 gtcaaggccg gagcggtgag ctgcgtactg gggcgcaata ttctgagagc cgcgacggcc
       61 ggtcctgtag cgttgtcgct gttgtctcgg gtgttgggac gttacgagta acgcccacgc
      121 gctaagctgg tgcgtagcaa catcaaacca aggagcatga tgagcgaatc cgacgattgc
      181 ctgttctgca agatcatcgc agggcagatt cccagcagca aggtctacga ggacgagacg
      241 acctacgcat tcaacgacat caatcccaag gccaaggtcc acgtgctcgt ggtgcccaag
      301 aagcactacg ccaacgtcgc cgaacttgcc gccgacgacc cgaccgagct ggcccacatc
      361 gtctccattg cgcagaacat cgccgacaag gaatttcacg gcgcctatcg actggtgttc
      421 aacaccggtc tcgacgccgg ccagaccgcc ttccacatgc acgcgcacgt gatgaccggc
      481 gaaaagctcg acgagtagta ggggaatatc cgcttcgtgg caacgactac acgtaccatc
      541 accatcccgc cccagcttga ccccgtcgcc gtgctcggcc cggttgacga ggtcattcgc
      601 gaggtggagc gcgcctttcc ggacctgacg attatcgtgc gcggcaaccg cgtggcgatc
      661 gtctcccgct ccaagcagac cgaagcccaa gccgcacagg ccgaggattt ggtcaatacc
      721 atcatccaag ccgcctacac cgcgcctatg gacgccgaca ccgtgcgtcg catgctcgac
      781 cagaacgtgc tcaagaacca cgtgcgcctc gaacatcccg gtcacggccc ggtgcgtgac
      841 aaactggcgc aatcgacctc aaacgaacgc gcccatgcac gttctgccgg ctcccacgac
      901 ccgacgtacc gcaagcccac ggttcccggc atcatcacct ttgctgccgg cgtgccggtc
      961 cgtgcgaaaa ccgccggaca agtggcctac gtgaacgcca tcgaatcgca caccatcacc
     1021 ttcggcattg gccccgcagg caccggcaag acgtatctgg ccgtggccaa ggctgtgcgt
     1081 gccttccagg acaagcagat tcgccgcatc atcctcacgc gcccggccgt ggaggccggc
     1141 gaaagcctcg gctttctgcc cggcacgctg aatgacaagg ttgacccgta tctgcgcccg
     1201 ctgtacgacg cgcttggcga catgctcggc gccgaccagc tcaagcggta catggacgac
     1261 ggctccatcg aagtggcgcc gctcgcctac atgcgcggcc gtacgctgaa cgacgcgttc
     1321 gtgatcctcg acgaggctca gaacaccacc gaacagcaga tgaagatgtt cctgactcgt
     1381 ctgggcttca acaccacgat ggtcatcacc ggcgatatct cgcaggtgga cctgaccgtg
     1441 ccgcgctcgg gactggccac catcgaacgc atcctgggcg gcatcaacga catcgcattc
     1501 gtgcatctca aaaccgagga cgtggtacgt caccagctgg tcggccagat cgtggccgcc
     1561 tacgaccgtc actccgccat cgccggcgac caccgcgaca tcgaacgcaa gggccgccgc
     1621 aaccataccg acttcgatgc ccgccagcaa gcggattcaa ccaaccaaag cgagacagac
     1681 gatgagcgtt gacgtcacca atgaaaccca gtgggtcatc gaccccaagg tcttctccga
     1741 tttgggtatt tgggtactcg accagatgcg cgtgagcacc cagtccgacc tgaccatcat
     1801 gttcgtggat cccgatccca tcgccgaact gcacatgcgt tggatgaacc nnnnnnnnnn
     1861 gacgtgatga gcttcccgat ggacgagctg cgccccggcg acggcaaaac cgtgatggaa
     1921 ggagtgctcg gcgacatcgt catctgcccg tgggtcgccg cccaacaggc tgccgcagcc
     1981 gggcacagca ccatgcagga gatgctattg ctcaccatcc acggcatcct gcacctgctc
     2041 ggctacgacc acgtgacccc cgagcaggag cgccagatgt tcggcctgca gcgacagctg
     2101 ctgctcacct tcttcgccct acgccacgat gccaacgtgc aggccacgct gcccgccggc
     2161 acgcccgacg cgctggccct gtacgacgcc gcgcacggtg ccggccgcga cctcgattcc
     2221 aaataagctc ccaaatcaat cacaccaaat ccggattatt cgataccgtt caaggacatt
     2281 caaggacacc gaaaaatcat tatggagtat tccagtacca cgccgatcgc catcaccgtc
     2341 attcttgtac tcgtggcggc cctgttcgtc tggctctcgc tgaccatggc cgccgctgaa
     2401 ggcgccgtgg cccgcgtaac gcgagccagc cttaacaatc tcatcctcga ggtgcagacc
     2461 gactccgaag caagccagtt cctcaagatg aagaagatcg acaagattca tcgcgtgcaa
     2521 cggctcatcg ccgaccgtta cgccaccgcc ggcagctgtg cgttcttccg catcgcctgc
     2581 aatgtgctcg acggcgtact ggtgtcttcc atcgcatccc tgtggggtgc ggaactgtgg
     2641 attgaactgc tctccggatt cctgttcgcg attatcgtag ccgtggtctc cgtactggtc
     2701 cgcccgcgca ccgccggcat ggccaagccg gtcggcatca tgatttccaa ctcgcgcatc
     2761 gtgtcgttgg ccaccaagct cacgccgttc gcacgcatcg gtggcgacaa gtccggttcc
     2821 aagggaccgg gccgccgctc caaggactcc gatctgtccg acgacgagga gcttgagaag
     2881 attcagctcg agcagggcaa ggccgccatc gaccgactgg tggagtccaa cgacttcgat
     2941 ccggaagtct ccgaaatgtt gcgcaacgtg ctcaccctgt ctgatacgtt gactcgcgag
     3001 atcatggtgc cgcgcaccga tatgatctgc atggagcgcg actccacact gtcttccgcg
     3061 ctgaaactgt tctcccgctc cggcttctcc cgcgtgccgg tgatcggtga ggatgtggat
     3121 gacctgatcg gcgtggccta tttgaaggac gccgtgcgtg ccaccgcctt caacccggcg
     3181 gccatggaac gcgcagtcga atccatcgtc cggcagccgc tgcttgttcc cgaatccaag
     3241 ccggtggacg acctgttcca tgagatgcag cgttcccgcc agcatgtggc cgtggtggtc
     3301 gatgagtacg gcggcatcgc cggcatggtg actatcgagg atgccatcga acagatcgtc
     3361 ggcgagctgg aagacgagca cgaccgcacc cagcgtactg agccggagaa aatcggagac
     3421 aacaagtgga agatgcccgc ccgtacgccg attgccgatc tggaggagat cttcgagatc
     3481 gacattgatg aggacgatgt cgataccgtg tacggcctgc tgaccaagat tctgggccgc
     3541 gtgccgattg tcggtgcctc ggccgtgacg cgcggtctgc gactgaccgc cgtggactcc
     3601 gccggacgcc gcaagaaggt gtccaccatc gtcgtagaac cggcgcacgt cgagggcggc
     3661 gaggaaaata acaagaccca tgaggaaact gacggcgagc ccgtcgaagg cgatgatacc
     3721 gccagcaagg agtgatatga ccgaccagaa caacagcacg aacgacgtcg atgacattga
     3781 cgtcaccgat gcttccgatg ttgccggcgt tgccgaagtc gacgcgaccg gtgagacgga
     3841 gaacgtgacc aagcaggacg tgccgtatcg ctccggattc gtggccgtgg tgggccgccc
     3901 gaacgccggc aaatccacgt tgatcaacgc gttgatcggc aagcagattg ccatcgcatc
     3961 ctctcgcccg gaaaccaccc gtaaggcgat ccgcggcatc ctgaccgccg accacgcgca
     4021 gctcgtgctc gtggacacgc ccggtatcca ccgcccgcgt acgttgcttg gccagcgtct
     4081 gaacgacgtg gtcgacgaat cattggccga tgtggacgtg gtggcctnnn nnnnnnngtg
     4141 gaggccttcc tgctgcccgc cgaccaggaa atcggccccg gcgtcaagcg tattctttcc
     4201 cgactgcgta ccgacttcgc caccaagcgt gaggacggta cgttcaagtg gcgcatcccg
     4261 ctgattgcca tcgtcaccaa gatcgacgag cttggccgtc agcaactcat caacaagctc
     4321 atcgaaatca acgatttcgc cgatttctcg gacatcgtgc cggtgagcgc cctgaagcac
     4381 gacaatctgg ctgaagtgaa gaacgtgnnn nnnnnnncag atgtacccgg acgatcagat
     4441 cagcgaggag cgtccggaag acaccatcgc cgaactcgtg cgcggtgcgt tccttgagac
     4501 cctggacgac gagctgccgc attcgctggc cgtggtggtc gattccatcg actatccgga
     4561 agacaacgag accggtcagg gctatgacgg caaggcgcag gtcaacgtgt ccatttacgt
     4621 ggagcgtgac tcccagaagc cgatcatcat cggcaagggc gcctcgaacc tcacctacgt
     4681 caagaagaag ctgcgcaccc cggtcaaccg catcgtcggc caaaaagcca agctcgacct
     4741 ccacgtcaag gtagccaaag gctggcagtc cgaccccaag caactagaac gcctcggctt
     4801 ctagggcgta tagacgaaaa aagctccctc gtcagaggga gcttttgcta tacggtttac
     4861 ttccgcttgg tgtacatctg ggtgttgagg agggtggagt agcgcttgat gaccttgggg
     4921 cgaatcacct tcatggaggc ggtcatgagg ccgttctcct gggtgaactc ctcgggcagg
     4981 atgatgaact tgcgcacgga ctcggcgcgg gagacgccct cgttggcctg attgacccac
     5041 tgctgcactt cggcgcgcac ggcggcgttc ttggcggcat cctccagccg catgttctcg
     5101 tccagaccct tggcggccag ccacgggcgc agcgactcct cgtccagcgt caccaatgcg
     5161 gagatgaacg gacgcttatc acccagcacc agcgcctggg agacgaactc gcaacgctga
     5221 atgacttcct cgatcgggcc gggggagacg ttcttgccgc cggcagtgat gatcagatcc
     5281 ttcttgcgcc cggtgatgta caggaagcca tcgttgtcga tacggcccaa gtcgccggtg
     5341 gcataccagc catcttcggt gaacgacagc tcagtggcct cgtcgttctt gtgatagcgc
     5401 gggaacacgg cacgaccctt gacctgaatt tcgccgtcct cggcgatgcg cagggagaat
     5461 ccggggaatg cgatgcccac ggaaccagcg tggaacggcg tacccagcgg gctgaacgcg
     5521 cacggtgcgg tggtctcggt cagaccatag ccctcgtata caggcacacc cgcgccacgg
     5581 aagaaagcca gcagttccgg gtcaagcggg gcaccgccgg ccacaatcca cttggcgcga
     5641 ccgccgagca cttcgcgcag cgaagagtag acaatcggat cgaaggcagc gcggcgagtg
     5701 cgggtcagtg cacctgcctt gccattggcg gagacctcct tcatatagtt ctgcgcggcc
     5761 accacggcgg aggcgaacac cacgcccttg gggccgtggc cggccttctg ggacgcggcg
     5821 ttgtacacct tctccagcac gcgcggcacc acgatcatga gagtgggctt ggccacctgc
     5881 agatcgctga tcagcgtctt gatgccggtg gcgatgtaga tgcggacgtt cgaggagacg
     5941 acgatgtagt tgatggcgcg ggcgaaggaa tgggcctgcg gcaggaacag caggatggtg
     6001 ttcctcttgt cgtgcagcag atcgggcatg taggccggca gattcagcgc ggtctggcag
     6061 tagtgctcgt gggtcatctc cacgcctttc ggcgcggccg tggaaccgga ggtgtacacg
     6121 atggaacaca ggtcggtctt cttgaccgaa tcgatgcgct cgtccagctc ctcgtccgag
     6181 accgtggtgc cgtaggcctt gatctcgtcc aagccaccgg tctcgatggt gatgatgtgc
     6241 tccagggacg ggcactcttc gacggcccca tccgccttct tacgcatgtc cgtgtcctgc
     6301 acgatcagca gtcgggcatc ggagttgttg acgatattgc gaatctgctc ggcggaatcg
     6361 gtgtcgtaga tggtggcgag cacgccgccg cacgccatga tcgccgcgtc ggtcaggtcc
     6421 cagtcgtagg aagttcggca catgaatgcc acggcatcgc ccttcttcag gccgtaatga
     6481 atcaggccct tggccacggc acgcacgtca gccaggaact cgttggcggt gacggtcacc
     6541 caatcgttgc cggacttgta ggtgtacagc ggctcatcgc ccatacgttc ggcgcggtcc
     6601 gcatacaggt cgtagatgga cgtaccttcg tccagcggcg gattgccttc ggtggaggtg
     6661 gtgagcaggc cggtggcggg gtcgatgtcg gtgacggtca ggtggccgtc gtcccaatcg
     6721 tcgctgtgag gcaaagcgat attggtctcg accggcacgt cggcgttgga caggtaatcg
     6781 ggctcgtccg gggtgtcgtc gtggaccggg tggacgaagt cggatcccaa cctgagcgcc
     6841 ttacgggtga gattgatcgt gtgctgcgtg aaggaattga taaccgacaa gtcttgctcc
     6901 tgtaaacctt tcagtgtcgt ccagccatga ggcgaacgct tctcaaagtt caaattgtaa
     6961 tcgcccaaac cgaactcgtc accttacgca accgtaggat tctggcgggt taggggagtg
     7021 cgtggcaaaa tattgcacag gtcccgatat tgttgagtaa ccgtgagcta cgaaacggtg
     7081 attgtcgata attattgagc gcttggttgc taatcgatgc caggtgattg ttggacgact
     7141 tccgagcaat tcatccgcaa tgcacatgga cacattcgta tttcttacta atttcgggtt
     7201 cgtttctatt tcgattggcg gaatctcaag tcatggtctt tacctttcga ctacacttac
     7261 catgcggata accatgtcca ttttgaagca gaacaagaaa gggctctcat ggcgcaaaac
     7321 ccagaagcca ccgtggtgtt cggcgttttg aacgaaacgt ccgaaacgga atcccgcgtc
     7381 gcgctgacgc cggatatcgt cacccggctg aagaggagcg gtgtctcctc actgattgaa
     7441 gcaggtgccg gcattgctgc cgactacacc gatgaggatt acgaaaaggc cggtgcgaag
     7501 gtgaccagcn nnnnnnnnng ctcgccgaag ccgacgcgct cggttttgtg gaccgaccgt
     7561 ccgccgagac cgtggccaag ctcaaggccg gccagtgggt cattggcatg ctcggttcct
     7621 tcaccgatgc cgattacgtg gccgcgctcg agaaggccgg tctggtgggc atcgccatcg
     7681 aaaagctgcc ccgtaagctt tccagcgccc agtccatgga tgagatgacc tctcagaact
     7741 ccgtgatggg ctacaaggca gccatcaccg ccgccgatgc ttacggttcc ttcctgccga
     7801 tgatgaccac cgccgccggc acgattcgcc cggctaaggc gctggtgctc ggtgccggca
     7861 tcgccggcct gcaggccatc ggtacgctga agcgtcttgg cgcggtcgtc accgcgtacg
     7921 acgtgcgtcc cgcctcccag ggcgaggtcg agtccctcgg cgcgaagttc cttgatctgg
     7981 gacttgactt ctccaagggc cagggcgagg gcggttatgc ccgcgcgctg agcgccgaag
     8041 agcaggctca gcagcaggct gccgtcgacg aaaaggccgc cggctttgac atcatcatca
     8101 ccaccgccaa ggtcccgggc cgcaagcccc cggtgctgct caccgcagcc ggcgtcaagg
     8161 gtctcaagcg tggtgccgtg atcgtggact gcgcagcatc cgacctcggc ggcaacgtcg
     8221 aaggctccgc cgttggtgag caggtcaccg agggcggtgt caagctgatc ggcgctccct
     8281 acctggcctc cggcgttgcc accaccgcat ccaacctgct ttcgcgcaat gtggccgaca
     8341 tcctcgtgca cttcgtgcgt gacggcaagc tcggccagga tctcaccgaa gaactcgacg
     8401 acgcgctggt ggttgccggc cgcgccgccg ccccggcagc tgctgccgat gccgagaagt
     8461 ccgagaagta aggaagaagg aataccatgc ctactctcgt cgtttccatt accctgttcg
     8521 tcctcgcgct gctcatcggc atcgaggtca tcggcaaggt gcccgccacc ctgcacacgc
     8581 cgcttatgtc cggcgcgaac tccatccacg gcatcgtcat cgtcggcgtt gtgatcgtgg
     8641 cagccgaagc caatagcccg ctggcctacg tgttcatctt cctggccgct gtgctgggca
     8701 ccatgaacgt cgttggtggc tacgtggtca ccgaccgcat gcttgagatg ttcaagtcca
     8761 gcaagaacga gaagaagggg gagtcgaagt aatgacctcc gcaaccgttg gcgccatcga
     8821 catcgtcgct tggttcgtct acctgttctc tgccgtcctg tttgtggtcg gtctgcattt
     8881 catgaattcg ccgaagaccg cccgcaaggg taaccagatc tccgcgttcg gcatggtcgt
     8941 ggccgtgctc atggcgttca tcgtgctctt cgccaagggc ttcgtgaaca ttgtcgccgt
     9001 ggtggtgctg gtcgtcggca tcctgatcgg tgccgtggcc ggtgtcgtct ccgccaagaa
     9061 ggtgaagatg accgatatgc cgcagctggt cnnnnnnnnn naagctgcag ggtatcaagt
     9121 gggtcaagaa gctgaacctg ccgggcaagg gcgtatggaa cattctgttc gccgtgctgt
     9181 ccgtcgtctc gctggtcatg ctgtgcgtcc agcccgaaca gcgtctgctg tggtcgattc
     9241 tgaccaccgt gttcgccctg tgctacggct tggtgttcgt gattccgatc ggcggcgccg
     9301 atatgcccgt cgtcatctct gtgctcaacg catgcaccgg tacggctgtg gctatgtccg
     9361 gtctggcgat tgacaatgtc gccctgatcg tggccggcgc actcgtcggt tccgcaggtg
     9421 tgaccctgtc catcctcatg gctcaggcca tgaaccgccc gctgctctcc gtcttggccg
     9481 gtggcttcgg cggtggtgct gacgccgccg ctgctggcga cggtccggaa ggcaccatga
     9541 aggagaccac gccggacgat ctggccgttc agctcgtcta cgcgcagaag gtcatctt
//
LOCUS       DAWBMW010000051         9539 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_1745_length_9539_cov_2.690110, whole genome shotgun sequence.
ACCESSION   DAWBMW010000051 DAWBMW010000000
VERSION     DAWBMW010000051.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 9539)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9539)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9539
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1745_length_9539_cov_2.690110"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    7512..7546
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1836 a         2890 c         2905 g         1873 t
ORIGIN      
        1 gagcaggccg gtgccgtcag gggtgagcgt ggactcgaaa ccttcgtcat cttcgacggt
       61 ggtgagcagc tcacgcatgg cgatggcctt gtcgctgcgg tcggccggat tgatcacgcc
      121 gccctgaccg tcctccaacg ccagcacatc ggtgaacacg atcacgccgt gcacgcgcag
      181 cagacgggag gcctgggcga acgtggcggc atagttctcg gcgtcgcccg ccaccacgat
      241 cagatcgtaa tcattggcgt tgaggcgcgg cagaaacacg ctggccgggg cgttcaccgc
      301 acgcaacgtg gtgtcggtct cgtcctgcag ctcggcgaac gccttgcgaa tcagcgcgat
      361 gccctgggcg gtggaatcga ccgcagtcag caggcccttg ttatccagtg catgggcgag
      421 ctgcaaggtc tccaccagcg agccggtgcc cacggcaatc accgaagcgg cgccggtgat
      481 gcgcaccagc atgcccagca gacgggcctg cgccgccgaa ccctgcaact ggccggactc
      541 gtcggcggcc gtacgggtgt cggtcaacgc atcggattca cgttcgcgcg cgtgttcctc
      601 ggtgaattcc catgccttgg cgaggttggt gtactgcgtt ctgtccataa ttcgttcttt
      661 catgtcttct ctcgtgcggg cacccagcgg atgtatcgac gcatgtgctt aggcgatcgc
      721 cgcagccagc cgcggcgcgg cttagcgctc gcggacctta gccacgatct gccacatctc
      781 ctcgcccaca tcgatgccgc gcgggcagtg gcgggagcag gcgcgcacgg actggcaagc
      841 ggccacgccg tcggcggtgt cgatggcatc catacgctcc atggccttgg tgtcgcgcga
      901 atcgttgacg aaacgggagg cccaaatcag cgcggccggg ccgatgaagg cgtcgccgcc
      961 ggcgtacacc gggcaggcgc cttcgcacac gccgcaggcg atgcagttgc tcagcagctc
     1021 gtatttggcg agttgttcgg ggttctgcag gtattcgaac acgtccacct tgccttcggc
     1081 agtggtggcg agcacgccgt ccgcctgaag atacggggtg agcttcctga tctggttgag
     1141 catagggtcg atgtcggcga tgaggtcgcg ctgcggcggg aagccgggca gggacgccag
     1201 ttcgatgact cccagcgaac cgtttgcggc tgagttctgg gcggccgccg cgtttgcgac
     1261 gttttccgcg gaatcgtcgg attcctcagc ctcggtgccg gtgtgtcgga acccttcgtc
     1321 atcgacttcg ggcatggcgc cgggttgctt ggcccaatcg cggatggtgg ctgtgcacag
     1381 cagggtgggg gtgccgttga tggccacggc gtccgaaccg cacatgccat gaccgcacga
     1441 ataacggaag gccagcgtcg ggtcgacggt gcgtttgatg gtcagcagac aatccaacac
     1501 agtatccgaa gggcgcgcgg gaatcgtgta ctcctgcacc cactgcttgc cgcgcggacg
     1561 acggcgcgcc gccgaatccg agccgccgcc gaacggggag cctttcttgg caaacgggct
     1621 gccaccgcgt gcacgttcgg cacgctcggg tcgcggcgtg aaacgatgca cttgcagggt
     1681 aaccgtcgtg ttgctcgcct cagccatgtt ctgcctccat gaaacagtgg gatgtcaagt
     1741 ccaatattag ccaacctatt gtgccacaag ccctaggcgg cttgggtatt accaccagcg
     1801 gatcagtact cgcgcttctt gggcggatag tcggtgatgt gcaccggctt gaaacttacc
     1861 gaaccataat cggcgaccat cgagtgagca aggaaatgct catcgtcgcg ttcggggaaa
     1921 tcaaggcgtt gcatggatcc gcgagattcc tgacgggatt cggaagcgtg cagcatgacc
     1981 tcggccagtt cgatcagatg acggacttcc cagatggcgg tgacctcctg attgaaagcg
     2041 gcggtcttgt catgggcccg caaggtatcg gcaaccggtg tgatgtcacc gttgatctgg
     2101 gtcaacgccg tatcgatggt ctgagcggtg caacgtacgg ccagagcctg ctccatcact
     2161 gatccgagtt gggccatgag ctgataagga ttggcggtgg gcactccatc gtctgaatca
     2221 tctattggcc cggcgagcag ttcgtcaagc tccgctttgc gagaatcggc agcctgatcg
     2281 gcggcatcgg tcagcatgtc atcggccgaa tcatcggcca tgggggagtc aaccgggttc
     2341 tcggcgatgc gtgcggccat cgtctgacca gcgcgtgtgc cgaacaggca ggcgtcgagc
     2401 agagaattgc cgcccagacg attcgcacca tgtacggaga cacaagagca ttcgccagcg
     2461 gcgaacagtc catcgactac ctgcacggca ccgtcggccc aacggtacac ctcgccgtcg
     2521 gtggtgatgg ggatgccgcc catcgtgtag tgtgcagtgg gcttgatggg tactaggtcc
     2581 ttggccggat cgaggttcgc gtactgctcg atggtttcga ccacctgcgg cagcacttcc
     2641 ttcatacgtt cggggtcgat gccggtcata tccagccaga cgcagtcctt cggaccatcc
     2701 gggtccttcg ggtcggcgac gccgcgaccg gcgtcgatct ccgccatgat ggagcgggag
     2761 accacgtctc gtgcggccag atccttatgc tccggagcgt agcgttccat aaacggctca
     2821 ccgtcggcgt tcctcagaat gccgccctcg gcgcgggccg cctcggacag caggatgccg
     2881 gtgtgcgcga gcccggtggg gtggaactgc acaaattcga tgtcctccag ctgcagaccg
     2941 gcggccaagg tcaaggccat gccgtcgccg gtcagatccc aggaatttga cgtggtgtgg
     3001 aacagtcggc ccgcgccacc ggtggcaagc agcacgttgc gcgcgtggat ggcctgaatc
     3061 ttgcccgaat gcgtgtcaaa ggccacgatg ccggccgcct gcttgccgtc gtcggcgagc
     3121 accaggtcgg tcacatacca ttcctcggcg aactcgacgc cggcggccac gccctgctgc
     3181 cacaaggcgt gcagaatctg gtggccgatg cggtcggccg cataggcggc gcgcttgacc
     3241 ggggcgccgc cgaactcgcg cgtgtggccg ccgaaccggc gctgggcgat atgcccgtcc
     3301 tcggtacggg agaaggccac gccctgacgc tccaggttga tcacggtctg cggcgcgtat
     3361 tcggcgagca gtttggccgc atcctggtcc accagccagt cgccgccctt gatggtgtcg
     3421 tagtaatgcc agtgccagtc atcggactcc acattgccca ggctcgcggc gatgccgccc
     3481 tcggctgaac cggtgtgcga acgcagcggc tgcagtttgg agaccacgag gatcttcggg
     3541 tccacgcctt gttccttgag ctcggtgatc tcgggggagc gcagcagtcc caacgccgcc
     3601 gacaggccgg ccgctccggc gccgacgatc accgcatcat acatatcttc caaacgtttc
     3661 ggactcatgg gttcgattct cgcacacccg ccgaacagcc ttcgagccgt gtcatggaag
     3721 ccgattagcc gacacggctg ctcagcttga gtatgccggc atcccgctcg atcgctccct
     3781 tcagctccaa tgcgccgagt agctcaagta tggtgccgat attgggaatc tcgtcgggta
     3841 ccgtctctcg cgccacacgc aatagtgcgt ccggggtgac gatgagatgt cgtttacggc
     3901 attcacgaat caatgccact aatgtgcgtt ccatttccgg cagcttggcg ataatgatac
     3961 gttgttgggc ttccttctcg ggatcgggct tcggcttgac acgaagatcc ggcatctgat
     4021 gcgacgaggg aagctcttca cccttgcttt tgtcgttacc ggaatcggca gttgcgctcg
     4081 ccggcccatc cttggattga gtgcgcgtag gctgagtatg cttggtcttc gaacgtttcg
     4141 aatgacctga atcttgtgac gactcctgtg acgccgatga ttccgaggat gctggcgata
     4201 acggagtcgt tgccggcact tccatggcct cctcgttttc ggacgagtcc tgacctgtgg
     4261 atttcgatat gcctgggcat gctgccataa cgggcttatg ccgttcatgg catatatcct
     4321 cggtggatgt ggcggcacac agaatcatcg ctcggtggtc gccgatcatc ttattgcacc
     4381 cggcgttgca aggctgattg atgtcaccgg gaaccgcata cacttcgcgc agcaattcgc
     4441 atgcccatcc cgcggtgttg agcgctccgg aacgcaaccg ggcttgcgtc acaatcaatg
     4501 tggaagacat ggccgcgata atgcggttgc gaagcagaaa acgacgggct tcgggaatcg
     4561 tcccggggca cagttcactg atcagtgcac caccctgcgc ctcgatgcgt tcgaacagcg
     4621 tgcggttacg catcggtccg atatggttga gcccgccggc gaatacggca acggttttgc
     4681 ccacattggc gggcattctg ccgtgcagcg cattcaacgc tccccaatgt gccgccgcat
     4741 ccgtgcccat cgcgcctccg gacacgacca gatgcccggc aacggctgcc tgttcggcta
     4801 cggtgtgtgc cacataccgg ccatattcgg tcacgtcccg tgaaccgacg atgccgaccg
     4861 gtttggcgca actggtcaat gcgcgtggat cgcctttgat ccacaaacac agcggtggcg
     4921 cccaatcgga gcgaatggac aggtcggcca actgcgaggg ccaacatgga tggccagggc
     4981 cgataatcca ttgcgtgcca tccatagtga accagtcggc cagttggatg atgtcttgcg
     5041 agggcaggtc catcatgcgg ttatgccagc aggccagtgc attgcggaag gagcgcatcg
     5101 cgttcgccga cgccttgcgg ccccatcgag tgaggccgtc cacgaacatg cggtcaatac
     5161 gtgacagcgc cgggccgcag atttcagatg gctggcttgg atgggattgc gccaacgcat
     5221 gccagaggga ttcggcgttt tccgcgcctt tgagcgtggc gtacatcatc acatccgcgc
     5281 catcaagaca gaacgtgagc gcggcacgat acaacgtatc cgtgggaggt acgggaatag
     5341 tgcgcgaaac ggcgagactc gtatcggtgt gatccacact tcctgcggtg ccgccagatt
     5401 gccggatatc agtagcgtta ctcatgtcat cctcgttctc atttcgatgc cctgatggac
     5461 gtcctgttca gtgggggata cgcggccggc cagatcggcg agcgtccaag acagacgcat
     5521 ggcgcgatca gccccgcgca atgtgagttg atgattggac agggcccgat tgaccaattc
     5581 catcgctttc agcgatgtat tggcgtgcag ccatttgccc gaagcttgcg cgttgcacac
     5641 ccatccatac tgtctgaacc ggtctttggc cacttgacgg gcacgaatca cacgagcttg
     5701 tatcccggca ctggactcgc cgattggctc gctttgcgcg gcaatgcgcg agactggtgg
     5761 caccgccatc tgaatatcca cacgatccag tatcggtccg gacaatcgcg agaaataacg
     5821 tatacggtct ttttcccggc aagcgcaacg ttcgccggtg ccgtaatagt atccgcacgg
     5881 acaggggttg gccgccatga tgagttggaa tgaggccggg tagtaggtgc tgccttttga
     5941 acgggaaagc gccacatacc cggattccaa tggttcgcgc aacgtctgca gggcgcgtgc
     6001 gctgaattcc ggtgcctcgt ccagaaacag aatgcctcga tgcgcgcgtg tgattgcgcc
     6061 gggaacggcg atgccgctgc caccacccac caaggccgcc gtgctggcgg tgtggtgcgg
     6121 tgcctcgaac ggcggcatgt cggtgatgcc gtactgaggc agtatgccgc acagcgatcg
     6181 aatcgatgcc acctccagct gttccgcctc gttcaacggg cacatgatgc cgggcatacg
     6241 tgaggccaac atggttttgc cgctgcctgg cgggccggtc atgatgagat tatgcccgcc
     6301 tgccgccgcc acttgcagtg cccatttggc atgctcctga cccagaacct cgttcatatc
     6361 gccacaatcg ttgggatgac ttgtgggcga ttgatccgtg gtggtctcgt cggtaaccgg
     6421 tgtatcggga atcgtatagg tggcatcgcc gcccatcagt tcgatgagct cacccacatg
     6481 cctcacgccc actacatcca gtccatccac catcgaagcc tcgtcaagat tgcgatgggg
     6541 aacaatcatt ttgcgcacgc cacgttcctc ggcatgcagc atgatcggga gcagcccatg
     6601 aatcggcagc accgagccgt caagattcac ttcgccgagc acaatggtat cttctaggca
     6661 atcatgccct atggcacccg ccgcacatag gacgctggcc gcgatggcca gatcatgcga
     6721 cgaaccgcgt ttgggcatgg ccgcagggct catgttcacg gtcacacggg cttgacacgc
     6781 tgatttcacg cgctcgcggg cttcggacaa cgatgcatcc ggcaatccga tgatgctgaa
     6841 gtatggcaag ccagggctga tgaaagcctg cacttggacg atgaaggcct taaggccgat
     6901 cagtccgact gagagggtgc tgccgatagc catgtctact cctttcacaa tgtgtttcgc
     6961 aacgtggctg tttttttttg gggggggggg gcttcagaag gcattttcaa tgtgatgcac
     7021 gagcggcctg cccactcgaa gcacgatggt gacgacatcg aaacggaccg ccgaatgcgg
     7081 cacccgatta cgccggtcaa gtagccaatc gcaggcggct ttcctgagat tgtgctgttt
     7141 cgccggcgta atggcctctt gcggatatcc gtaatgcatg gaccggcgtg atttcacttc
     7201 gacgaacacc accgtgtact cgggattgag catcacgatg tccaattcgc cgtaacgggt
     7261 atgccaattg cggctcaagg tcgtccaacc gtgttcctcc agccacgcgg cggcatactg
     7321 ctcgccgagc gcgccgaact gtttgggcgt cagattgcgg tcgcccatcg tggccgcaat
     7381 ccgttccgaa ggcagcattg cctgcggatc aggtgaagcc agcatgggct ggtgaaaggc
     7441 aagaccggcg tcgatgccgg acttcgtcgt cggctgagta ggtggtaaag cgttcatgat
     7501 tccactgtgc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnctta tgtaagagca
     7561 caagcgcaac cggcaaatgt ggatactatg acgcggtttg gcgtgttgtg gataacgtgt
     7621 ggatgaagac tcggttatcc accattagtt cagaaggttt accaaggctc accagtcgac
     7681 ttccctgtga cgaaaaaaca acccatcgga ttgttcgctg aatacgtcag acccagtgag
     7741 cggagagggg agccaaggtg cggtcacttc accaaatcgg tgagttcgcc aaggttcagt
     7801 tcgaaactga cgccacgatc ctcgaaatgc ggctggttac gggtgctgac catggcgtga
     7861 cgcacgaact caccggcgat ttcggccgac tcggccaggc cgcggccggc catcaccgcg
     7921 ccgcacaggg cagaggcgaa ggcgtcaccc gtgccatgaa tcatgtatgg caacttctca
     7981 tgagccagct cgatcttgcc ggcaacaccg gtggaagcgc tggccacata gttgacgatt
     8041 ttgccatcgc catggtcaat gcccttcagc accacgttct tggcacccaa ctccagcaga
     8101 gcaccaagca gctcgttaac cgtggcctca tcaatatcct ggccaggata gtcacgcttg
     8161 gtgagaatgc tcgcttcggt gaggttcggc atcaacacgt cggcaccatc ggccagcgcg
     8221 cccattgcct cgcacagctc cggtgtgtac gtggggtaca tttcgccggc gtctcccatc
     8281 accgggtcaa ccagacgcag cgcgttcgga tattcgcggt agagacgctg aatgatcgcc
     8341 acctgatcgg gggagccgag gaagcctgaa tacacgccat ccagctccac gtcttccttc
     8401 tgccaggcat ccaagtaacc ggagagaata tccgtggtgt catggaacgt gaacactgca
     8461 tagcgtgtgt gcgccgagaa cagggcggtc ggcaccgggc acacatcgca gccggcggcc
     8521 gagagaatcg gaatcgcggc ggtcagcgag cacttgccgt agccgcacat gtcgtgcacg
     8581 gcggccacgc gcggaatata gttcggatca cggtcataca gagttacatc ggtcatgtct
     8641 gttcgaatcc cttcaccatg ttggcggaat aattcattgg cgatgtttga ctgtagtcgt
     8701 tcgtcatgta aaccatgtaa ccgggcggca gaaaaatgaa acagacgtca tcaccgagcg
     8761 ggcaaaccgt cttggtgacg ggtggaacaa gcaatcatca cggacacttg agccatgcgc
     8821 gaaaggcatg gcagcgatat caccgtgacc gcagtcgacc ttgaaagcgc atgcgtgcgg
     8881 cccggcgtgt cgcgataatt tcaacgactt tttcgtgact tctgacagtc gctcacgtca
     8941 atgattccaa ggctttataa acccttatag gcaagtccga taacgttcag cttgcgccgc
     9001 gcggcccaag cgtcgtaact tcataaccaa cacctcgcca accgcccgaa cacggcaggc
     9061 gggtggcaag gaaaaatcct cgcgacctag cacaaccaag gagaatacca tggccgagaa
     9121 caacaagaag taccgttttg aaaccctgca gctgcacgtc ggccaggagc aggccgaccc
     9181 ggccaccgac tcccgcgcgg tgccgatcta ccagaccacc tcctacgtct tccacaactt
     9241 cgaccacgcc gaggcccgct tcggactggc ggacccgggc aacatctacg gccgtctgac
     9301 caactccacc cagggcgtgt tcgaagaccg catcgcggcc ctcgaaggcg gcaccgcggg
     9361 cctggccgtc gcgtccggcg cggccgccgt ggaatacgcg gtgcgcaaca tcacccagtc
     9421 cggcgaccac atcgtcgcag ccaagaacgt gtacggcggc accttcaacc tgctgcgcca
     9481 caccctgccg cgcgacggca tcaccaccac cttcgtctcc gccgagaacc cgcaggagt
//
LOCUS       DAWBMW010000052         9538 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_1746_length_9538_cov_2.548877, whole genome shotgun sequence.
ACCESSION   DAWBMW010000052 DAWBMW010000000
VERSION     DAWBMW010000052.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 9538)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9538)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9538
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1746_length_9538_cov_2.548877"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    7766..7775
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1884 a         2915 c         2891 g         1838 t
ORIGIN      
        1 ggtttgggcg atgaaggcga gaacaccatc cgcgaactgc gctggaccta cggtctgcgc
       61 ccggtctaca agaccgtcga cacctgcgcc gccgagttcg acgccgccac gccgtactac
      121 tacagctgct acgccgacga aaccgagctg cgcccgcgcg aacgcgaggc tgtgatcatc
      181 ctcggctccg gcccgaaccg catcggtcag ggcatcgagt tcgactacac gtgcgtgcac
      241 gccgtgcagg agcttggcaa ggactatgac accatcatgg tcaactgcaa cccggagacc
      301 gtgtccaccg attacgacat gtccgaccgc ctctacttcg agccgctcac cttcgaggac
      361 gtgctcgaga tctacgaggc cgagaagaag atgggcccgg tcaagggcgt catcgtccag
      421 ctcggcggcc agaccccgct gtccctggcc gcgcgtctga aggctgccgg cgtgccgatt
      481 ctcggcacca ccccggaatc catcgacttg gccgagaacc gtgagctgtt cggcgaagtg
      541 ctcaagaagg cggagatgaa cgccccgcgt tacggcaccg ccctgagcct cgaagaggcc
      601 aaggaagccg ccaaccgcat cggttacccg gtgctcgtgc gcccgagcta cgtgctcggc
      661 ggccgtggca tggaaatcgt ctacgatgac aagcagctca acaagtacgt ggaccgcgca
      721 cttaccgagg ccaaggccga taccgtggtc tccggtcgcc tgccctcccc gctgctcatc
      781 gacaagttcc tgcaagacgc catcgaaatc gatgtcgacg cactgttcga cggcgaggaa
      841 ctatacatcg gcggcattat ggagcacgtc gaggaggccg gtgtccactc cggtgacgcc
      901 gcctgcaccc tgcctccgtc caccctgtcc gacgaccaga ttcgtcgcct gcgtgaaggc
      961 acctacgcca tcgccaaggg ctgccatgtg cagggcctga tcaacgtgca gtacgccttc
     1021 atggccaaca cactgtacgt gatcgaagcc aacccgcgcg cctcccgtac cgtgccgttc
     1081 gcctccaagg ccaccggcgt ggcactggcc aaggccgccg cccgcatcat ggccggcgaa
     1141 accatcgccg atcagcgtgc caacggtctg ctgctgccca agggcgacgg cggcgacatc
     1201 caccccggcc agcaggtcgc ggtcaaggaa tccgtgctgc cgttcaagcg cttccgcacc
     1261 ccggtgggca agaccgtcga catcctgctc ggacctgaga tgcgttccac cggtgaggtc
     1321 atgggcttcg accgtgactt cccgcacgcc ttcgccaaga gccagctcgc cgcctacgac
     1381 ggtggcctgc cgacccacgg caacgtgttc atctccgtga acgataccga caagcgtcag
     1441 ctgccgttga tcgccgttcg tctcgaggag cttggattca agctctgggc caccgagggc
     1501 accgcctccg tgcttcgccg ttacggcatc gagtcgaaca tcgtggacaa gatcagcacc
     1561 cgcgtggaca ccgatccgga agccccggtc gaggtgcatc acgctgccgg atccgtgggc
     1621 aagaacgtgg ttcagctcat cgaggaaggc aagatcgaca tgatcctcaa cacgccgaac
     1681 tcgcgcggct cccgttccga cggctactcc atccgtgccg ccgccattgc cgccgatctg
     1741 ccgcagttca ccaccatcac cgagttccag gccgcactgc tcgctatcga ggccgtgaag
     1801 cataatgatt atcagatcat gagcattcag gagcattcga agcagctgtt cgaattggag
     1861 cgacgggagt tctgatgact acactcagca gtgaggaatt gcaggcacaa cgttcggatt
     1921 tcggtctgcg attaagcaat tcaatggcca aatacgggcc gctgtgtgtg ggcattgacc
     1981 cgcatcgcaa gctcctcacc gactggggat acaacgtcga cgccgaaggc gccgaactct
     2041 tctccatgcg tatgctgcag gccgccaacg ggcgcgccgc agcagtcaaa ttccagacgc
     2101 cgatgttcga gcgctacgga gccaaaggtt tcgaagcgct cgagcgcgtg ctctacgcgg
     2161 cacgacaaat gggcgtcatc accatcgtcg attgcctgcg cggcggcctg tccaccacga
     2221 tttccgccat cgcagacgcc tatttcaagc ccggtgcacc gttgctggcc gacgccatca
     2281 cgctgctgcc gtactatggc gcgcgttcgt tgaacggcct gattaccgaa gcgctggaaa
     2341 acggacgcgg cgtgttcatc gcctccctga cctccaaccc ggaaggcatg agtctgcaga
     2401 ccgcgttgcg ccagagcggc gattacaagg gacgtaccgt ggcctacggc atcgcctcca
     2461 ccgcgcagaa gcataacgtg gatatcgaag gcatgggctc ggtgggtctg atcatcggtg
     2521 ccaccatcgg ccaatggatg caggattccg gcgtggaccc ctccaagttc accggtccga
     2581 ttctttcccc cggttacggt tggcagggtg ccgaggccaa ggatctgaaa accgtgttcc
     2641 gcggtacgcg cggcaacgtg ctggtcaccg tgtcccgctt tatcgcctcg catgggccgg
     2701 atatcgccgc actgagccaa gccaccgagt cgattgcgct tgacgtacgt caggcgctga
     2761 tggaagccac tgtcgaaggc tcggaaaacc agtaatcaga acggaatcag atgtcgcggc
     2821 atgcgtacgt gtctatctca tcgcgtacgt cgcatcacga ttcatattct tccctgttcg
     2881 tcggggctgt agcagttacc ggtcatctcg ccggtgagcc gttaccacgt tggattcata
     2941 atcgctgcac accacatcat ctccggagga acccatgact gaaaacaccc atgctggccg
     3001 tctaatcgtc ctgactggcc ccaccgcagt aggtaagggc accgtggaag ccaagctgcg
     3061 cgccgaccac cccgaagtgt gggtgtccgt ctccgccacc acacgcgctc cgcgcccggg
     3121 cgaggttgat ggcgtcaact attggttcct cactgaagac gagttcctgg cccgtgaggc
     3181 ggccggtgag ttcctggaaa ctgcagtggt gcacggcatg gcccattatg gcaccttgtt
     3241 gaagcctgtg gaggaacatc tggccgccgg tgtgcccacg attctggaaa tcgacctgca
     3301 gggtgcccgc cgcgtcaagc agcgcgccgc cgagctcgac ctcgaagtgg tgtacgtgtt
     3361 catcgcgccg ccaagcttcg aagagctcga gagccgcctg atcggccgcg gcaccgaaac
     3421 cgccgaacag caggcccgtc gcttggagac cgccaaggtg gagctcgccg ccgaaagcga
     3481 attcgacgtc accatcgtca acgaaaccgt cgatcaggcc gctgccgacc tctggtccgt
     3541 catcgccaag gaatacgggc tgtaaaacta cctctttacc accaactgct ggtggaggat
     3601 ttcggcgctg tttttggccg gcgcctgctt ccagcggatt gcttcggttt cgtgatgctc
     3661 ggtgacgtaa tgccgcatcc attcgaagca ctgctgggtt tggatggcat cgcttaatgc
     3721 gcggtgctcc tcattgtccg cgatgccgaa gcgtacgatc aggtcggcca gacggtgatg
     3781 ccgctgcgcg gggaacaggg cgcggctgat ctgcatcgtg tcataatccg cgcaggcaaa
     3841 gccgcagccg gcgatatgcc gggtgttgta atccaggaat cgcagatcga agctgacgtt
     3901 atggccgaca atcggctcaa cggcgggacc ggcagccggg gtagcgagcc atgcgatgaa
     3961 tcggggaagc acctcgtcaa ccggatcgag atcggccacc atcgcatcgg tgatgccggt
     4021 cagcgtggtg atctgacgcg gaatcggccg caagggattc accagctgct ggaattgatc
     4081 gacgatatgc ccctcccgca cgcgcacggc accgatttcc gtgatggcgc aactgttcgg
     4141 gtaaaagccg gtggtctcca aatccagcgc cacataatcg gtggggaaga cggcatcgtc
     4201 catggcgatt cgcccgccgg caccatgatt gggctgggat ggttggccga aaacggtaat
     4261 gccggaagtg gtcacggagg ctcctcatat cagtcggtgt tcggtgactt attgtaccgg
     4321 cagcccggtc tgtcggtaca atttttccct cccggatttt ctcggatata ttggttaggc
     4381 ggccggcaat tgcgcggcgc atcgggaagg gagcacagct gatgaaacag atagtggcag
     4441 gcatcgtggc gcatgtggac gcgggcaaga ccacgctgtc cgaggctctg ctctaccgta
     4501 ccggtgagat ccgcaaattg gggcgagtgg atcacggcga tgcgtttctg gacaccaatt
     4561 ccttggagaa ggcgcgaggc atcacgattt tcgcgcacca ggcattggtg gaacacggcg
     4621 acctgcgcct gaccttgctg gatacgccgg ggcacgtgga cttcgcagcc gaaaccgaac
     4681 gtgtgctgcg tgtgcttgat tacgcgattc ttgtggtctc cggcacggat ggcgtgcaag
     4741 gccataccga gacactatgg cgtctgctgg ccagatacgg tgtgccgacg ttcatcttcg
     4801 tcaacaagtg cgacgccgcc ggattcgacc gcgaggccat tctggcacag ttgcgcaaac
     4861 gcctgagcga tgcgatatat ccgctgccgg caatggagca gtccactgag ggcagcgcgg
     4921 taccgttgga cgcgttcgcc gaagacattg ccaccctgga cgaggaggcg atgaacgact
     4981 atctggaaca tggcgcgctg tccgtgggca gattgcgagc gatgatcgcc gtacgcgaac
     5041 tgttccccgt atatttcggc tcggcattga aactcgaagg ggtcgaggaa ttcctggacg
     5101 ggctggagac gttcacccgc gaacgtgaat ggccggccgc cttcgccgca cgcgtgttca
     5161 aaatcgccca cgacggccag cacaaccgta tgacgtggct gcgcgtcacc ggtggtgcgc
     5221 tcaaagccaa ggagatcgtc tcttcatcct cgtgcgctgc ggcgtcatca gcaccgtcgc
     5281 cggtacaggg ggatgatggc acggtatggt ccgagaaagt cgatcaggtg cgcgtctaca
     5341 atggcgccaa attcgagacg gtcaccgagg tgcctgccgg atcggtgtgt gcggtgaccg
     5401 gactgacgcg gaccttcccc ggcgaagggc tgggagcgga accggacgcc gaatcaccgt
     5461 cgctgcaacc cgtgttgacc tacacggtgt tgcccgccgg agcggaatcc gatacggccg
     5521 gcacctcggg agcggccaaa cgcggtgacg ccggccataa caacgtgaat caggacgatg
     5581 ccgagcatga tgccgccaag tcgggaaccg gccgcaatga ggagaacgac accgccggcc
     5641 agaccgcggc ggacaatagc tcgccggccc gtccccagtt cgatgatctg accttgcata
     5701 aggtcatcac cgcattgcgt gagctggaag acgaagaccc gttgttgcat gtggtctggg
     5761 tggagcggtt ggccgagatt catgtgcaat tgatgggcgc ggtccagctg gagatcatcc
     5821 agcagaccct gcacgaccgg ttcggtctgg atgtgtcgtt tggccccggc tccatcctgt
     5881 accgtgagac cgtcactgcg ccgatcgaag gcgcaggtca tttcgagccg ctccgtcatt
     5941 atgccgaagc acatatcctg ctggaaccag gggagccggg cagcggagtc actgtggcct
     6001 cggccctgtc cgaaaacgat ttggaccgca attggcaacg gctgattctg acccatctga
     6061 ccgagcgtga gcatctcggt gtgctcgttg gcgcgccgct caccgatatc aagatgactt
     6121 tggtggctgg ccgcgcgcat ctgaagcata ccgaaggcgg cgacttccgc caagccacct
     6181 atcgtgcgat tcgtcagggg cttatggaag ccagagctgg agtcagtggc cggcccgaga
     6241 cgccggtcga gagccgtccg gacaccgcag gggagggcaa ctgccgactg ctggaaccgt
     6301 ggtatcggtt ccggctcgaa gtgccagcag acatgatcgg ccgtgccatg agcgacatcc
     6361 agcgcatggc cggcaccttc gaatccccgg tcaccgatgg cgaatacgcc gtgatcgaag
     6421 gcgaggcgcc ggtgtccgag atgcgcgact atgcgatgga tgtgaaccag tacacgcacg
     6481 gccatggccg tttctccgcc actttcggcg gctacaagcc atgccatgac gccgaacagg
     6541 tcatcgcagc cgccgcttac gatcccgaag cagatctgga caacaccccc gactcggtct
     6601 tctgcgccca cggtgccggc taccccgtca aatggtataa agtccccgaa ttcgcccacg
     6661 tcgactacgc catgtgaggt ggtgactacc aaatctgcgg ccgaatgaat ccgttggtct
     6721 ccaagaaccg ttgaatatcg cgcatttccg gcatattaat ggaatgggcc atgcccggat
     6781 acacttgttc ggtcaacgtg ccgtgctcgt gccagaactc acccatcgcc gtcacatcgg
     6841 ctttcgggaa gatgtcgtct gcagcaccat gcccatagaa caccggcggc ttcaacgaag
     6901 ccaattcggc atcgcccgca acggaccccg gtgccagcca tccagagcag gagacggctg
     6961 cggcatatcg cttcggattg aaacgcagca gatgcgcggc caagaggccg ccctgcgaga
     7021 atcccatggc aaccaccggc cgagtggcgg gaatatgctc cgcaacccat gcatcaatgg
     7081 cttgggcggc ttcagtcgcc tgcttgtcta atgattcgcc ctccggcaca ccttcatgcg
     7141 cccacgcacc gaaccatgta tatcccatgc cataggcgat gggtgcctgc aaactggcat
     7201 aatcagccga accggcaccg caatagttca gcagatcggg caaatcgtat tcattggaac
     7261 cccagccgtg caacagcacg aacaccggag cggtttcggg accggtcaaa cgggtcagag
     7321 ctttggtgat cttcatagcc tcagtgtatc caccgtttgg gcgacaataa tgccctgatg
     7381 ttcgcccatc gagtaacgtc ccataagtaa tgcgtatagc gcagcggccg tgccacgtgt
     7441 tctgatactc tacgatgaca cggcaggggg acaggtatgt tcggcccccg aaatcgtaca
     7501 tgggtggcta agaccgttta tgcgcaggag aagaaacgag aagcaaagga aggagcgcgc
     7561 ccaatgacat tcgattggtc gttcgtgcag cgttacgcgc ctttcttcgt caacggcaca
     7621 ttgatgacct tattcatatc cgtgtttggc atattgcttg ccatcgcggt gggtctggtc
     7681 tgcgctggcg tggaaatcgc gcgtatcccg gtcgcccggc aaatcgtgag aatctacatc
     7741 gaactcagcc gcaatacgcc attgcnnnnn nnnnngcgta gtatggtccg ccgaaacctg
     7801 cgcgatagtc ggccttggat tcttgggcgg ttcctacatg gccgaggcaa tgcgcggcgg
     7861 attggaagcg gtgcccaacg tgcagcgcga aaccgcgtac gtgctgggac tgacttccac
     7921 gcaaaccatc acccgagtca ttctgccgca agcagtgtcc accgccattc ccggcgtagt
     7981 ggccaatgtg atcttcctgt ttaaggaatc gagcgtcgtc tccgccattg cgctcgccga
     8041 cgtgatgtac atggccaagg atctgatcgg catgtattac agcacctatg agtcgttgtt
     8101 catgctggtc gtggcctatc tggtgattct gctgccgatt tccatcttcg gcacatggtt
     8161 ggagcggagg ttcgattatg agcggcgctg aaatactgct ccagccgggc gtattcccac
     8221 gtctgttgca aggcctgtgg gtcaccgtgt ggatagccgg cgtatccgta ggcgcgtcca
     8281 tcccggtcgg cctgttggtg ggctggttga tgacgctgaa gaatcccgtg atccgggccg
     8341 cgatgcgcat ctacctcgac ttcatccgca ttaggcctca gctggcgttg ctgttccttg
     8401 cctactacgg ttttgctcga tggttcgatt ggaatctcga tgccaccggt gcctgcgtgc
     8461 tggtgttcat cctgtgggga ggcgccgagc tgggcgatct ggtgcgtggc gcattgcagt
     8521 cgattcccaa agtgcagtac gaatcgagct acgtactggg gttgagcccg tggcagacct
     8581 tctcccgggt gattctgccg caggctgttc gccgtatgct gccggcatcc gtaaacttgg
     8641 cgacacgtat cgtcaaaacg acatcattgg ccgtgctgct cggtgtggtc gaggtcatca
     8701 aggtcggcca gcagattatc gacgcgaacc gattccagtt cccgactgga accttgtgga
     8761 tttatggtgt gctggccgct gtccattgtg gcccgccgac tggaaaagag gtggtcgaat
     8821 gactgaaccc gaaacgacct gcaagggttt cgaggtgaat catctggtca aacagtatgc
     8881 gaatgccgac aagccggtgc tggatgacat ctccttcaag gtggagcatg gttcggtgct
     8941 cgtggtgctg ggtccttccg gctcgggcaa gtcgacgctg ctgcgcacca tcgccggtct
     9001 ggaaccgatt cagggcggca cgatttccat caacgatcag gtgatcgatg ccggcaagcc
     9061 tggcacggaa cgagccggac gatccgaccg gtccagtgaa ctgcgcacgc gtatcggcat
     9121 ggtgttccaa agctacgatt tgttcccgaa caaaacggtg ctcggcaaca tcaccatggc
     9181 gccggtgctc gtgcagaaac gcgataagtc cgaggtggag gctgaagcga tccggttgct
     9241 tggccgcgtg ggacttgccg atcgcaagga ctcttggccg cacgaattgt ccggcggtca
     9301 gcgccagcgt gtggccattt gtcgtgcgct gattctgcac ccggaagtac tgctgctcga
     9361 tgagattacg gcggctctcg accccgaaat ggtgcgtgaa gtgctcgacg tggtgctcga
     9421 actggccaag tccgggcaga cgatgcttat cgtcacccac gaaatgcagt tcgcgcgagc
     9481 catcgccgac cacatcatct tgctggacgg cgggcacatc gtcgaggaat cgtatgat
//
LOCUS       DAWBMW010000053         9321 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_1784_length_9321_cov_2.583315, whole genome shotgun sequence.
ACCESSION   DAWBMW010000053 DAWBMW010000000
VERSION     DAWBMW010000053.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 9321)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9321)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9321
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1784_length_9321_cov_2.583315"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    7414..7423
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1929 a         2814 c         2696 g         1872 t
ORIGIN      
        1 cgtagtcgcg gcggtacgct gccgctcatc gagcccctca agaatcccct gcgcctgttc
       61 catgcgtcca ttgtggcacg attcgcgctc acggcacgac tcatgcctat ggctgaatcg
      121 ggctcacgac aaaacatcgc cagaatcatg cgttttgcgt gtcattctgc ggtgcgaatc
      181 gccacggatg tattagtgtg gaagggtgat gaaacgtagc gattatatgt tggcggcact
      241 cgcctcagcc gtcctgccga atctgggggt ggccggcgta cgcgagaacg tgcaggccag
      301 cgcaaccgac gaggccaaag gcatcgatca gaccgtgatc caagatgcct caggcaagtt
      361 gtacgacgta tacgccacta ataccaagga agggcgtacg cgtctgattc gccgtgtgaa
      421 agcggcgcag acactggcag ccgcgcgcga gcctggtggc ctcggcttcg atatggaccg
      481 tgtggtcgcg ttcgccccgg gagacgtttc ccgacccggc cagacggcca ccgcacaggc
      541 cgtggggcag cagacccagg cggagtccac accgctgacc ccgcgcaagc ccggtcccac
      601 aggcgagacg accgtgatga ttgccgtcca ccgtgatggc caggcgcggc ctctcgacct
      661 gttgacattg gatgactgcg cggccgtcgg caccgccatc ggagccattc atcgcctgcc
      721 ttcgactttt ctggccaatg ccaaatatcc ggtagttacc accggtcaga ttcgttcgca
      781 gctgaccgcc tggattcgcc gcttacgctc cgccggccat gttccccaag aaatcaccga
      841 cagctgggcg cgcatcatgg acaccgaggg attgtggtcg ttctccacct gcacggttca
      901 cggcggattt tccgacggcg acttcctgtt ctccggttcc accatcacca cggtgaccaa
      961 ctggcaggat atgcaggtca acgatccggc acgagacctt gcctggattt tcggcaagct
     1021 cgacgaatcc catcgcaacg ccgtgctttc cgcatacggc cgcatgatgg gctctcgact
     1081 tgacgatctc atcatgctca gggccaacct gtggctgcag atggaacagg tgggcgaatt
     1141 cattcaggcg ttgaaccgtg cggacaacga tcacatcatg cagttcaagg cacaggtcga
     1201 acgactcgca caccagttgg gcgtgatcaa gcacactccg gcaccggccc cagccgcagg
     1261 gtcggccgcc gcctcgtcaa cgaagcccgg tttgccttcc acaatcaccg tcggcacatt
     1321 gttgcaggac gaccgtcatc aggcacccgc acaaccgcag cagtccgaca ccggtaccgc
     1381 ggcggccgag gtctcggacg acacgcccga tcccgatcgc accggttccg ctgacatcgt
     1441 ggccgctggc gacgtggatc ttaccccgga agtcgaaccc gatgctgacc ggaccgccaa
     1501 tcacccgcaa acgaatgtct ccggcatcgc aaccggtacg ggatcgacca aaccgcagcc
     1561 ctcacatggc gcttcccatt cttcggtgac gatcacgctg aaggagctcc tcagtatgaa
     1621 cgggcatagc ggcacgacgt caaccgacgc ttcccctgcg tctcaatccg ccgccccgac
     1681 cgacgatgcg gtgaatgacg atactccgga cagtctcgtt cagtacggag cctcgagtgc
     1741 ccaaaccacg gtgattccgc tgttggagcg ggaagaacgc gctctgcgcg atgcccgtgc
     1801 cgggttggac gggtatgaca gcgagggcaa tccgctggcc gaatcgtcca catcatccaa
     1861 ataatgtcgt gtacggtgtt ccgcgccacg ccgagcgcgt aacaccgcac atgacgacgc
     1921 agacacgcgg cacaatagag tacagaacct cggccggcga cggccactta ccatgatgtt
     1981 cgtcacaaag acggcagaac aaggagcctt atatggatat cgaactgggc attcagaacg
     2041 tggcacgacc ggtgaacttc agcaccgacg aatcagccga caatgtgagc caggccatcg
     2101 cgcaggccgt ggccgacaac gcgacgatcg atctgaccga tgacaagggc cgtcgcatca
     2161 tcgtgcccgc caaggcactc ggttacgcga tcatcggctc cgaaaccaag cacgccgtcg
     2221 gcttcggcac actgtgacca tgaatcgcgc ataagcggcc ttgggcatta tctcaaggcc
     2281 gcttttgtat tgctgaatca ccgatatagc ggaagcacgc cgcgatgaac gatctcttcg
     2341 acagtggcct cgtcggtgag gttctccccc agtttgttcg gtttgccttt gccatgccag
     2401 tcagacccac cggtgaccag cagctggtgc cggcggcaga tggtgagcag ccgttctcgc
     2461 tgctccggtg aattgccacg gtgccatacc tccagcccgt ccagtccttc gctgatcaat
     2521 gcctcgatct ggtcatcgga caacagtctg cggttacggc tcacatcacc ggcatgggcg
     2581 atcagaatca caccgcccgc tcccttgacg gcggccacca cttcatgaga ggtcggcgaa
     2641 ggcgtgggaa tatagtattt ggatgaggcg ctgaccgcat ccgcaaacgc ttccgaacga
     2701 gtccgatata cgccggcact caccaaagca tcagcgatgt gaggccggcc aatggtggtt
     2761 ttgctaccct ccttgacttg ggcgagcacg gattgccagg taatcggata atcacgagac
     2821 aacaattcga ccatgcgctg cgtgcgttgc aaccgtgcgg ctcgagtatt ggcgaacaag
     2881 tccgtgatgt gctgattcgt gggatcatac tggtagccga gcatgtgcac ggagacgttc
     2941 tcatccgtgg cggtgatctc ggtgccgcag agcaacggca aaccgaccct atgagccgcg
     3001 gcttcggcct ccggccagcc ggcggtggtg tcatgatcgg aaatggccac gcccttgagt
     3061 cccaatgcct tggattgttc ggccaacgtg gccggcgttt ccgtgccatc cgagaaggcg
     3121 gtatggcagt ggatgtccca cccgacggcg ggcggttcca aaggcatcac atatccagtc
     3181 ataccttcca tcgtaacctc atttcagcat tcccacgcaa ggcgcagaca ggtcgtcacc
     3241 ttctgccaac ggccgaaacc agacattcgg cacccagctg acaaccgcag aggctaatct
     3301 ggagcgcagc gctatcgaaa gggacatgat gactcgtaac accgcaaccg acacatcatc
     3361 cagcaccacg aacaccacgc cgcttggctt ggcggaggca cgaccgttga ttggatggcg
     3421 ccacagtgcc acttggacat atctcatcat gctgattgcc tcggcagtgg cgttgggggc
     3481 ctcgctcatt ctgtccgctg agactttgca attggctcgg catccggagt ccgcactcgg
     3541 ctgcgatctg aactcggtgg tgtcctgttc ggcggtggcc cagtcctggc aagcggaaat
     3601 cgcgaagttc ggcgggctga gctatccgaa cgcgttcttc ggcatcgccg ccgaatcggt
     3661 gttcattacc atcgcggtga tcggtttggc gcgtgtgaag gtgccgcgtt ggttcgcgac
     3721 ctgcacgtgg ctgggcggac tggccgcttt ggcttactcg tactggctga gcacgcagtc
     3781 gctgttcgtg attcacgcgc tgtgcccgtg gtgcctgacc ctgatgttct ccaccaccat
     3841 ccagttcatg gcgttgagcc atgcgaccgt ggccgtgcag ggattgccgt cgcgtaaggc
     3901 tgtggcggct gatgatagcg acggtgaggc tgaggtcgcg gcggtgccgg ccggactcaa
     3961 taagtattac cgcctcaaca tcgacctcat ggtcgacata ctgtggatcg tggcgatcgt
     4021 ggtactcatc atcgtcactg aaggcgcggc cctgttcgct gcctgattcc tttacaacaa
     4081 atcgatatcg gcgaccagcg tggctgctcc ggtcagcttt acgtcgctgt cggtgacgtc
     4141 cacgcgcagg gtgccgccac gtacggtgat gtcccagtgg ctgatgccgg tcttggcctg
     4201 cagcacaatg cctgtggcgc acaggccggt gccgcaggac agggtttcgc cgcagccacg
     4261 ttcgttgacg cgcatcgtgg cttcacccac gccggtgctc tgatcgatgt cgtcgacccg
     4321 cacgaattcc acgttctggt cggattcgat gaccggagcc accactggtt tggtgaccaa
     4381 atccagtgat tcaaccactg gcagggacgc gaaggcatcc tcgatcaccg cgaccacatg
     4441 cgggttgccc atatccacaa acgtaccgcg cgccgagccc tcagtgccgg ggatggtgac
     4501 ctcgtaggcg tcggtttcgc cgatcttcca ctcccccatt tcgacctgga acacgttggc
     4561 tccgtacggc ctcatattgc cgcgtggcgt cagaatcttg acgccggcac gggtgccgag
     4621 gcggaacggc tcggtggagt cggccacccc ctcacgctga gcgaacaagg tgatggcgcg
     4681 tgtaccgttg ccgcacatct ccgccaacga tccatccgca ttgcgatagt ccatgaacca
     4741 tttcgcgccc ccggccagta cgtccgaaag ctgatcatcg cttaaatcag acacgaaatc
     4801 aggccgtgtc aggcgaatca ggccgtcacc gccaatgccg aagtgacggt cgcacaggaa
     4861 tcggacctca tcggcggtag gctcgaattt gccggacttg tcgaggtata ccacgaaatc
     4921 attgccggtt gcgtgtgtct tggttactcg ttgcggaaga ctcatggctt tcagagtact
     4981 ctagggagcg aaacaacacc gcaaaacaat cggctttgtg atcggtcgcg gcaacatacg
     5041 gttgcagtca tggctatagg gaggattcag gcatgagctc ttcggctcca atcggcgtat
     5101 tcgattcggg tcttggcggt atttccgtgg ctcgccaaat cgccaaggat atgccggccg
     5161 agcatgtgct gtattttggt gattcggcca acgcaccgta cggcatcaag acgcctgaac
     5221 aagtgagggc cctgagtttc gatattgtcg aacggttcgt gcgtcaaggc gtcaaggccg
     5281 tggtcatcgc atgcaacacc gccacttccg ccgcggtgaa cgatttgcgc gaacactacg
     5341 atattccgat catcggcatg gagccggccc tgaaagtggc gtgcgaccgt ggtgacgtgc
     5401 cttccgaccc gcatcatatt ccccagaggg tgattgtggc ggccactccc ctgaccttgc
     5461 gtgagcggaa gttcgccaaa ctgatggatc gattcgattc caacaacacg attttcaagg
     5521 agccttgccc cgacttggtg gagatcgtgg aatccgggcg gctcggcgac catgacctgg
     5581 tgatgcgcac gctgcacggc tacttcgacc agtacgacat ggagcatatc gactcggtgg
     5641 tgctgggctg cacgcatttc gtgttctacc gcgactactt ccgcgagctg ctgcccgaac
     5701 gggctgctgt catcgacggc aatgagggta ccgtgcgcca tctgggcgtg gtgttggagt
     5761 ccctgggcaa actggcaccc gaagacgcga ccggcggcgt ggagctcgcc aattccgatc
     5821 cgtccgaacg gattgccgag ttgtcacgca aattgctgaa cgtatgagcc gttcttggct
     5881 cccctctctg agggaagcca aggaagaacc agacaaattc acagcacact gcccatatcg
     5941 ttgtccgtgc agcacgcgta agctcatacc agattcaagt gaacaggggg ttggtatgac
     6001 aaagacggcg atgattgatg tcggcggcgg gttccgcgcg attttcggct gtggtgttat
     6061 ggaccgcatg ctggaggacg gcatcgacgt ggatatgtgc tacggcgttt ccgccggcgc
     6121 ggccaatatg acctcattca tcgcacgcca gcacggccgc aaccacacct tctacaccaa
     6181 gtacgccttc cgcaaggagt acgcgagtgc ggaaagcttc ttcaagaacc acaactttgc
     6241 caacctcgac tacatctacg gcacgctgag caatcacgat ggcgagaatc cgttggattt
     6301 cgcggcgttc gaggccaatg ggaccgggtt caccgtggtg gcctgcaatg ccgaggacgg
     6361 ctcgaccaag tacttcgaca aatcgcgcgt gcgttttgat gatttcgaca tcatcaaggc
     6421 ttcgtccgcc gtgccggtgg cctgcgagcc gtatgtggtg gatggcgtgc cgtatttcga
     6481 tggcggcatc gctgatccgg tgccggtgca gaaggcgctg gaggacggct acgatcgcgt
     6541 gattctgatt ctgtcccgct tgcgtgacga agtgcgcaag cagcagaagg atttggctcc
     6601 ggcccgcatc ctcgaacgct cccaccccgc cgcggccgag aagctgcgca tgcgctacaa
     6661 gacctacaac gacgaactcg aactcgccaa gcattacgag gcggaaggca aagtgctgat
     6721 cctcgcaccc gaagacctgt atggtctcaa caccctgaag aagaacttcg agggcctgga
     6781 aatgatgtat cgcaagggat acgcggcggc cgaggccatt ccggcattcc ttcaggcata
     6841 gcgatagtga agtccctcgc ttggaggggc ttcactatcg atggatgggg attcgcaaac
     6901 ggggtgatta cttcgtctcg gaagcggtga gcatggcctt ggtgacctgc tcgtagagat
     6961 cctgataacc gccgggcgtg gaggcgggca gcaccacggt cttggtgttg ttcgattcgg
     7021 acagcgagcg catcacgtcg aggtactggt tgaacagcac cacgttgttc acgtcgttga
     7081 tgttcatgcc cacagcctgc agactcttga tctggtcgac gataccgttg gcgatctcgc
     7141 ggcggtaatt ggcctggccc tcaccctgca agcgagtctt ctccgcatcg gcggcggctt
     7201 gggtctcgat ctggatacgc tgggcctcgg cacgctgacg ggtggcttcc ttttcacgct
     7261 gggcggcatt gatggagtcc atcgcgttct tgacctgtgg gctcgggtcg atggcggtga
     7321 tcagcgtctt gactacggtg aagccgaaac ggctcatttc ggcgcccacg gtcttctgca
     7381 cgtcgaacgc cacatcgtcc ttgcgagcaa aggnnnnnnn nnngtccaaa gacagtgcgg
     7441 ggatagcgga acgcagcgcg tcctccatgt aggagcgcag ctgaccggcc gggtcacgca
     7501 gctcgtagta ggcggtggcc acatcgttcg ggttcacgcg gaactgggtg gaggccacca
     7561 cggtgacgaa cacgttgtca agagtcttgg tttccagctg cacgttaagc tggttgacac
     7621 gcatgttggt tttcatggcg atgcggtcca cgaacgggat gcggatgtgg atgccggcga
     7681 actggacctt aaggaacttg ccgaagcgtt cgatgatgta cgcctgctgc tgcggcacga
     7741 cgaagagtgc ggcagacagc agaacaatga gtagcacgat gataatcagc ggaccagcaa
     7801 agaatacggc catgatgttt tcctttcacc cacgtcgaaa ccgccctcgg cctcgactcg
     7861 tgatttccat cgtatcagcg ttcaaaacaa agcggtagac atcgtacgca tatgtgtaca
     7921 actgttcact atgtgtgcat gctggcttct tataaaagaa gtgcggcatc caatcagtat
     7981 gctgcggcca tgcctgagcc gacagccacg gcgccgatac cgtcctccag cgcggcaagc
     8041 cgcttttcca gtgcatcgac agtgggattg gccacacggg agtactcgaa gccgtcgatg
     8101 tcaccgcccg cgagcgcatc gccgcgcgcc gcgtcaccca ggtcgaatgc ggccgaggca
     8161 tagattggcg gcacagcggc acggccgtta tccagcggct cgtagccggc gtggatggct
     8221 tgcgttcgga aatcaacgcc cattaatcct tcttcctcat gccgctctat tgtggcgggc
     8281 tcgactctat tcccgaactt agcaagtcat ggtacgcgac accggcacat cggtatcgca
     8341 attgtcaatg cgcgcttata agcaatggct atagaatatc cgccgaatgc cggaacacac
     8401 caagctgcct agccctcttt cagcacctcg gccgcaatat tacgctgcct caaataattc
     8461 aaagccgcgt caatccgtac cggttcatca tcgaaaatgg ccacgatgcc ctctaccgga
     8521 gtatccgcga cgatgtcgat atctccaaag attatgttga catcgatgcc gaacccttta
     8581 gtaatttgcg acaccaacgc ttcagaaact tccttggaca catattgcat ccggaccaat
     8641 cgttgacctt cagacaatct catcaaaagg gagtcatggt tctcttttca tatctggcga
     8701 taacgcatat caaatcagca atcagcgcaa agacgctcgg tcgctccatc cggataacaa
     8761 ctttgccgtt tcgcctaagg tgtcgatgac atggtcaacc ggtgcgtcag aaagagcttc
     8821 agcatagttc tcgatgcgat agtcgaaata acgatcggcg cccagcgcgc tgctctccga
     8881 aagcaccggg acgaacgccg tcagcattcg taacgccttg ggagcgctca aacgcgcggg
     8941 gctcatcacc gtggcacgcg gaaccggcgg cgccgaactt gcccacaccc cccaaggaaa
     9001 tcactctgct cgacatccat caggcagtgg aatccacgaa cctcgatgat gtgatcggca
     9061 tccacgagag agggaatcac acatgccccg tggctcggaa tatccacgac gttctcaaag
     9121 atgcgtatgc tccagtggcc aaagccatgt cggattccat gagggaagtt acgcttgcca
     9181 atatgcttgc cgactaccga aatcgcattg gggtcaaagc ccggcaactg gaacagtagt
     9241 gggcaccgaa gcgtaagccc cctactctct gatccgcata aacagataag acgtgcgcca
     9301 tcaagaccga tcgatgacgc a
//
LOCUS       DAWBMW010000054         9151 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_1825_length_9151_cov_3.272537, whole genome shotgun sequence.
ACCESSION   DAWBMW010000054 DAWBMW010000000
VERSION     DAWBMW010000054.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 9151)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9151)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9151
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1825_length_9151_cov_3.272537"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1733 a         2712 c         2756 g         1950 t
ORIGIN      
        1 ccggctccgc gaacggattt ccagtcacac agaatgccgt gaacagtcag ccatgcctgt
       61 tgcgcggcga agagaaaaga agaagcagtg gattatcctc tctttaccaa gctggccgcc
      121 gagttcttcg gcaccgccat tctgatgatc ttcggcaacg gctccgtggc caacgttgag
      181 ctcaagaaca ccaagggcca ccacgccggc tggctgaaca tcgccatggg ctacggcttc
      241 ggcgtcatgt tcccggtgct catgttcggt agcgtctccg gcgcgcacat caacccggcc
      301 atgaccatcg cccaggccgt caacggcctg ttcccgtgga gcgacgtgct gccgtacatc
      361 gtcgcccagc tgctcggcgc cctgctcggc cagctcatcg tctacatcac ttactacccg
      421 cactacaagg agaccgagga cgccgaggcg attctgggca ccttctgcac cactgacgcg
      481 gacaaccaga aggccaacta cttcctcaac gagatgttcg gcaccctggt gctcgtcttc
      541 ggcgccctgt gctgcctgtc cctggcttgg ggcaagcagg attacgccgc cgcctccatc
      601 gtcgtcggct tcattgtgtg gggcctcgtg acctccatgg gtggcccgac cggccctggc
      661 ctgaacccgg cccgcgacct catgccgcgt ctgctgcacg ccatcctgcc gatcccgcac
      721 aagggctcct cccgctgggg cgaggcctgg attccggttg tggccccgat tgtcggcgcc
      781 attatcggcg cttggctgtt cgtgttcttc ttcgccagct gattcgttga ttgattcata
      841 tatgcaaatg gcctccgctc gttttgggcg gaggccattg tcgttgaatc aggtaaacgc
      901 agagactact gctccaggaa cacctgcggg gccttcatga agttcgtggc acagtgttcg
      961 ccgcagaagt aataggactt gccctcatac tcgcgcgtga tcgcgtcggc attcaccgcc
     1021 acggtcatgc cgcacaccgg gtcggtggcg gtttcgccgt ccgcgggggc ggtgtggtgc
     1081 atgtgcattc ccatatccat agtgttctcc ttgttggttg gattattgga ttggtcagat
     1141 acgtgattgg tttgattcag cgtggtgcga tcgtcgatga tgacggtcgg ctcgtgaggc
     1201 gcgttggatg tggcggagcc ggtgatgtcg gcggcgttgg ctgattcggc cgatccggct
     1261 gattcgacat ccgccatgct gctgcccgag ccagcggtgc tatcggcagc acccacgttg
     1321 atgttcgctc catgcagccg gttcgcgttc agcaccaggc acactgagct caacgccatc
     1381 gccagacccg cgatcatcgg gctcaacagc cagccggtga acgggtagag tacgccggcg
     1441 gccaccggaa tgccgatgac gttgtagccg aacgcccacc ccagattctc gcggatgttg
     1501 cgcatcgtgg cattggacag gttgatggtc ttgatgacgc cgcgcaagtc gccgctcatc
     1561 aaggtcacgt ccgccgactg catcgccacg tccgtgccgg tgccgatggc gatgccgaga
     1621 tccgcctgag ccagcgcggg ggcgtcgttg atgccgtcgc ccaccatcgc aatcagagtg
     1681 cgggattgtg cggccgtgcg ggaggtgccg tacgcggatt tggcggtggc ctcgtcgcgt
     1741 tcgcgctgca gtttggcgat ccagtaggcc ttgccgtccg gcttgacctc gcagatcacc
     1801 gtatcgatgc cgacttcgcg ggcgatgcgc ccggctacct cggccttgtc gcccgacagc
     1861 atcaccgtgc ggatgccgcg tgcgcgcaac gccgcgatgg cctcgcgcga gccttccttg
     1921 actgtgtcct gctcgtagct cggcttgtcc cagtcggcgg ccgcgtccac cgcgccgcgc
     1981 gtgatggtgc cggtcttgtc gaacaccacg gtgccgatgc gacgcgcctg ttccagcgcc
     2041 ttggcactgg tgaccagcac gccgttggtg gcgcccaagc ccagcgagac cgtcaccgac
     2101 agcggcgtgg ccagtcccag cgcgcacggg caggcgatga tcagcacact gaccgcagtg
     2161 accagcgcgt gcgccagttg cggcgccggg cccagtgaca cccagatggc gaacgtccag
     2221 atggcgatga tcatcaccgc cggcacgaaa taccgggcga tcttatcagc cagttgctgc
     2281 accggcgcct tggtcgcctg ggcgcgggcc accatcgccg cgatctgcga gagtaccgtg
     2341 tccgcgccga cttgggtggc gcgcatcacg cagtcgccct tgagcagcac ggtggccccg
     2401 gtcaccgggt cgcctgcggt tttggagacc ggcttggatt cgccggtgat catcgattcg
     2461 tcgatacgcg cctcgccggc cacgatcacg ccgtccgtgg gcacgcgctc gccgttcttg
     2521 acgaccagca ggtcgccggt gacgatggcg tcgatgtcgg tgtccgtgaa atgcgccgga
     2581 ttacgccatt cgatgccgtc cacattgtct gcggaagtgg cgttgagctt gtgcgcggtg
     2641 cgcgggcgca gtctgataag cgactgcacc gccttgccgg tgccttcgcg ggccttggcc
     2701 tccagcaggc ggccgaccag caccagggtg atgaccacgc cgaccgattc gaaatacggc
     2761 tcgcgcgagc cctccgggaa cacccacggg gcgatgcagg tcaccagcga atacaggtag
     2821 gcggccgaag tgcccagcga gaccagcgag ttcatgtcgg gggagcggtg cgccagcgcg
     2881 gggaagccga ccgtgtggat cggacgcccg cagtagaaca tcaccggcgt gatgagaatg
     2941 gcctgcagcc acgggttcac catccaatgc ggcaccagcg cgggattggc catatgcagc
     3001 atggtgacgg cgaataccgg caacgtcagt accgcgccca caatcaccag cttcgtcagc
     3061 gccttgatct cattggcgcg ttcagccgcc tctcgccgct cggcctccgc cgcatccaca
     3121 accggagccc caacccgcaa gtctgaggga gagcccccct cggcttcggc cgagccgtca
     3181 gcacgcgggc gaagcccgtc cttatccgca ccgtccttac tctcctctcc ctcaaccctc
     3241 aacaacccat gcaccatatt catgccgcat gcaaacggat attcgcccgc cggcaacgcc
     3301 ggtagttcca cgtcggtggt ggcgttgccc ggcagcatgg cgtcaagccc cagatccgcg
     3361 aacaccacat gcgaagtgca ttcgccggtc tcctgtcgat cgaaagtcag cgtgatcggc
     3421 gtacccgtgc gcatgctgat cacggcgggg gagtacccgc ccttgacggt gattgttgcg
     3481 gattggcgtt cgccggaggc gtccatggcc ccggccgtgg ctttctgcgt tccgaagaag
     3541 aaccgcagta ccagccatgt caatgcggcc gccgctatca gcgccgtggc ggccgagaag
     3601 atgctgagct ccataaccgt tccttcccct tgtttgttgt actgaacaac agtgtatacc
     3661 ctatacccct agagggtata taatggggtg tgttcagctg acggataagt atcagggggt
     3721 ttgcgatgca tggttatgac gaagacaagg ccaaggtcat cgcacgattg cgccgtatcg
     3781 aaggacaggt gcgtgccatc acgcagatgg tggaggatga caaatactgc atcgatatcc
     3841 tgacgcagat ttcggcgtcg aattcggcgc tgaaatccgt ggcgttattg cttctggacg
     3901 atcatctcaa ccattgcgtg cggcagtcgt cgattcaggg cggcgaggtg gccgatgaaa
     3961 aacttgcgga ggcttccgcg gccattgcgc gtctcgtcaa atcgtgacga gttttaccgt
     4021 cattcaccat cgatgacata ctgggtacca tgtttgagca aataggaacc gtgattatcg
     4081 tcatcgtcgt gctagtggtt ggtgcagggc ttggcctctt cgccggcttc gtgctaggcc
     4141 gtcataaggg tcaggagatg gctcgaggcg tcaaaagcga ggagctggag gaggccaaga
     4201 cctcgctgga gaatgcgcga ttcgagaacg ctgagctcag tgctcgcaat gctgccgcac
     4261 aggctcagat tgaaggcgtg aaccagcaat tggctttcgc caagtcacaa cttgcccagg
     4321 cgcagcaggc tgagcagatt cgcattcagc aggaacgtga gcgtgcagct gcggaggcag
     4381 aacggaagcg tcaggcggat gctcaggccg ccgaggtcaa gcgtgctgaa caggaagcac
     4441 gtttgaaaga acagagcaag gtgctcgagg cgcttgcgcc agtggccaag aatcttgata
     4501 ccttgcagaa caaggtcacg cagatcgaag agggccgcaa gagggaaatg ggcgcattgg
     4561 gagcccaact caaggggctc aacgatcagc aggcccgttt ggacaaggaa accagttccc
     4621 tgtccgccgc gttgcgcaac aacaaggtgc gtggcgcgtg gggcgaggcc cagctcaaga
     4681 atattgtcga gtccgccgga ttgctggaac atgtcgattt cgacacccag gtggtggtca
     4741 cggatgccga tgggcgtacg ttgcgcccag atatgatcgt gcatctgccg ggaggcaaga
     4801 ccattccgat tgatgccaaa gtgccttatg ccgattacca gcgtgcctgc gaaatcccgg
     4861 acaccgccag ccccgaggat attgcccgcc gcgatgattt gctgcggtcc catgccaagg
     4921 cgttgcgcga gcatgtacgt gctctcggcg aaaaggccta ttggaatgcc tttgatgtag
     4981 cacctgattt tgtggtggcc ttcatcccca atgaggcact actgcaggcc gcactcgaag
     5041 ccgatcccac actgatggat gatgcgttct ctcgcaaagt ggcgctcacc tcgccggtca
     5101 cgctgtgggc cgtgctgaaa tccgtggcct atgcctggca gcagcagtcg ctcaccgatg
     5161 acgccaagca gctgttcgat ctgtcccgag aactgtacga acggttctcc acgctgggtg
     5221 attatgccat caaattgggc acccagatta ctaagacggt cagtgcctac aacaagttcg
     5281 cgtccagttt ggaacgccgt gtgttgccca ccgctcgcaa gctgcaaaaa atcgaaccga
     5341 ccaagatcat cgaggaagta ccgatcatcg aatcagataa aggcaatgtg agcgaactgt
     5401 ccgcccctga agtggtgccg gccgatgaag gtgatactca tctgactgcc tcaggcgata
     5461 gtctggagtc atgactgaat ctggattccg ccccactggt accccggacc ttgccgtcgc
     5521 gaagagccat aatgactttg ccatgctgga gccgcgtgaa cagctagcca ccttgctgaa
     5581 ggagcatgtg gtgggccgcc cgttctccga acttgccgcg gtgactttcg accaccgtgc
     5641 cgccactgtc atgggccatg tactgattga cgctttggaa gatgcgggct attcgattga
     5701 tgacttcgat gctgttggtg ccttgaccgc agcatctgtg cccatggtgt ctgccatgat
     5761 ccaggccgcc gcctctcgtg gcgaggacct agatggattc gtgatggact tcgtctaccc
     5821 gtccatcaag ggcccctcca tcgaaggccg ccgtgtggtg ctgttggatg cttggctgtc
     5881 cgaaaagtcg tacgtgcaga ccagctcgct ggtcaccctg cgcaacggca atgagctgag
     5941 cctcgacttt ggcatagtgg agcagctggg tgccaaggtc gtggccatcg cctcgctggt
     6001 cggtggcggt gccaaagaca tcaatgtggt caacccctcc accggcgacg agcaaacgct
     6061 ccccttcatc caggtcttcg acgaatccga actgcggtaa atccaccttt gacgtccctc
     6121 gcataaagtc agccgggctg agcagcgaag ctgactaaga ggaaccggtg aatagcgtta
     6181 tcgaggagaa agattgcacg cacccaacca gatttctttg gctgccaagg cttcaggtga
     6241 accggaattc cgtgaaatcg gccttgggcc gtggtctgag acccatcccg gtgagcctcg
     6301 tcccgatgat ccgacttctt cgaattatga cggtcgtttc gactcggtgc tgttgaacga
     6361 cggagaccgc cgcaacgtat tggaccgtta ccgctactgg accgttgctg ccattaaggc
     6421 cgatcttgac gctcgagggc gtcacgactt cgaagttgcg gtggaaaact ggacccacga
     6481 ttttaacatc ggttccatgg tccgcaccgc taatgccttc caggccaagc gcgtacacat
     6541 tgtcggtcca cataagtgga atcgcaaggg cgcgttgatg accgaactgt accagcacgt
     6601 cgaacaccac ccctccatcg ccgaactggt ggaaagctgg catcatcgca tcgccggtga
     6661 aatcgcctat gaacgagcca aggccggtgc cgccgccatc catgcgcatg atgtggctgt
     6721 ggatggagac aacaagcacc ttcatgagct gctggccgaa atcgatgctt gtgatgcacg
     6781 catcaaggag cttcaagccg cccgcgtcat cgccctcgac attattcccg gcgcagtgcc
     6841 gatggaagcc tatgccttcc ccaagcggtg cctgctgctc ttcggtgcag aaggccccgg
     6901 attgagcgag aaggccctcg cattggccga cgatgtggtc tacatctctc aattcggttc
     6961 cgtgcgctcc attaatgcag gcgctgcggc ggccgtggcc atgcacgcat ggattgccca
     7021 acacgccgta tcaggcgtgt agccatgcgc cgacaatcct gccgcatctg cccaatatgg
     7081 gccacattag aggcatgatc tacgccacca aatccatcgc cgcggcatta attcgcattt
     7141 gccgccggca tcgtcggcat cctgctcgcc gcaggcatca gcagctccgc gcagtccaca
     7201 ctggccgaag ccgttaaacc ggactactgg attcccgaat ggtggcagtc cggcctgatt
     7261 ctcatcgcct gggctgccgc cgcatggatc ggcggtctca tcatcaccgg aaaagcggat
     7321 atcagatatc aaataacgcg ggttactgct ttggcagttc tgccggttcg gccatggtcc
     7381 tggcagtttg tcatggctat ggctttggcg gttctgcagt gaccatggtt ctggtgactc
     7441 tgaccccatg gatttgacca ccgtggttca ctgcgcttaa ccagctccca tcactgttcc
     7501 agcatgaggt atgccggctg cccgcagctt attgagtaaa ggatgttgtt gcatcacctc
     7561 ctcataggtg aatcggacaa tggtgtcgac tttggcccgg tacagtgccg tctcccgttc
     7621 tttctccgcc tgaactgcac cacgaacacc cttgcggtca ttcatatccg ggttcacata
     7681 tttttcatag ccatcgaatt cgcccacgat aagccgattc tcaggtagcc gccagaggaa
     7741 atccacgcga tacagagcac cggtttgcgg gtcggtgatg ttttgttgca gttctggggc
     7801 taataggccg cctgcgatga tggtgcctcg tccaagtgac tcgccaccat tctcactcgc
     7861 cggattggca taatgcagta ggcggaatat cggcgtgcaa tcacgtcgca tagtgctgca
     7921 cacattcaga atgtccgtga tggcgacgcc gttgcgcagc gccgaatcga taatgggcag
     7981 tgcaaatcgg aagtcgacgg acagcccgca atccaccacc gttctggctt tgctggtcac
     8041 cgggagtcca ttggcatatt ctggcgtgct gcttgacgtg aagatgcgtt tgacctttgc
     8101 actgctggcg ttggggcctc gtgatgttgt ggcgagagtg atggtgccag catgaagatg
     8161 ccattggtgt tcaaaatcgt ggatggccgc tgccgtgacc ccggcgaaca cccaatccgg
     8221 atgtctgtta tgaagagttc gtgcaatatg tctgatacgt tcgtcgggtt gcagcgaatc
     8281 ccagtattcc gaagcggcgt acatgttggg gaatacacgt ttgagttcgc gcgcctgcac
     8341 gcgccgagcc agcgccgagc ggtgtgcctt ggttgttcca taagcgcact gttgcgttgc
     8401 ttcagccttc gtgatgagta tgtccactgc tttgtgatgt ttcatgattc gacggtatgc
     8461 caatggtgaa cacggtgtct atctgtctgg ggttatgtgg ttctagcttc ggtgcctaga
     8521 tgtgtgtttt tgcctgatta tcaggcatct gaccgtattg ggctgattat gtggattctt
     8581 ttgggtgggt atgcaaaatg tggataactg gcgaggtgcg cagggcgatt gtcgttcata
     8641 tgttcgatgt aggtgtgttt gggctcattg tgagcttatt agggtggcca tgcagatact
     8701 tcagtggggc acatttgcgg ttgggccgtg gcgttgcctc gctgcggggc gtctcagcgg
     8761 ggcgttttat tggttggacc gcggttttgt tttgctggga tggctgagcg gggcgtttca
     8821 tcggtcagct cgtggttttg ctctgctgcg agtcgtctca gtaaggtgaa cttgcggtcc
     8881 ggcaacggtt ttgccccgcc gaagaagcca gcagcggggc aaatgctcga ccggcttgag
     8941 cttctgattc gctgaagggc tccagtaggc gggaaaatga ggcttcccga caactgcgtg
     9001 tcacatacgg ctcatatagt tatggccatg ccaacatttt cacgcgaaga aatcgtgcat
     9061 ttgggcgatc tggcccgaat cgcgctcacc gatgaggaaa tcacccgtct gcagggcgaa
     9121 ctgaacgtca tcgcagactc catcaacaag g
//
LOCUS       DAWBMW010000055         8918 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_1870_length_8918_cov_2.488097, whole genome shotgun sequence.
ACCESSION   DAWBMW010000055 DAWBMW010000000
VERSION     DAWBMW010000055.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 8918)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8918)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8918
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1870_length_8918_cov_2.488097"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2973..2982
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7159..7168
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8248..8257
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1977 a         2625 c         2540 g         1746 t
ORIGIN      
        1 tgcttatgga agacacgcat ttcggcacga tctacgatgc cgaccagttg gcgcgcaatg
       61 aggttccgct gcaggctgcc gtctattttg acgacatgta cgtcgactcc ggtctgcaat
      121 tcgatacgct gtcccgtgtg ggacgatcgc actattggac caccaacgaa ttcgagcacg
      181 acggcgtaca tggttccgtg gtgttcaagc acctgttcga cgaggcgttg aacaggggag
      241 acctggaaga actgttctga cattcccgaa attctccgaa accatcgcat ccaaaacaat
      301 tacgtccatt agaaaagacg acaaggagga caccatgtcc aatccaacca tcggattcat
      361 cggatacggc aatatggcgc aggccatcgc cgaaggactc gtcgcagccg gcgccgtcga
      421 cggtgccgac atcgtggctt gcgccgcgca ttacgacaag ctggaaaaga ccacgatgaa
      481 agtgggtgca aagccattgc acaacgcggc cgaagtcgtc gcagcgtccg acatggtcgt
      541 catcgccatc aaaccgtacc aaatcgaaag tgtcatcaag ccagtcatcg gcgcgattgc
      601 cgacgaagac aaattcgtcg tatccatcgc ggcgggctgg gatcttgaca aataccgaga
      661 cttgttcggc gccgatttca acgcggcgca tatccaatgc accattccga acacgccgat
      721 ggccgtcggc aagggggtac tcgtcaccga agccgacaac acgctgaccg acacgcaagc
      781 cgaagtgttc gagcggctgt tctcatcgat cagcctcatc gaacgcgttg attccgcgca
      841 tatgggcatt ggcatgtgca tcgcaggctg cgcaccggca ttcaccgaca tgtatatcga
      901 ggcgctcggc gacgcgggcg tgaaatacgg cctgcaacgt gccaccgcct accgtctagc
      961 cgcgaagatg gtggaaggcg tcggtgcgct atacctcaaa aacgaaaccc atccgggcgt
     1021 catgaaggac gccgtctgct cgccgggcgg caccacaatc aagggtgtga cctcgctgga
     1081 ggaaagcgcg ttccgcggtg cggtcatcaa ggccgtcgac gccatcgaag cctaatacta
     1141 agctagggaa tcatgtctct tactatcgga atcgtcggac tgcccaacgt cggcaagtcc
     1201 accatgttca acgcattgac ccgcaacaac gtgctggcgg aaaactatcc gttcgccacc
     1261 atcgaaccga acaccggcat cgtgccgctg ccagacgacc gtctgccagt gcttgccaag
     1321 ctcgtgcata ccgaaaagat cgtgccggcc accgtcacct tcgtggacat cgccggcatc
     1381 gtcaagggcg cctccgaagg cgaaggcctg ggcaacaagt tcctggccaa catccgcgag
     1441 gcggacgcga tctgcgaggt cgtgcgcgcc ttcgaggacg acgacatcgt ccacgtcaac
     1501 ggcaaggtgg atccggccga cgacatcgac accatcaaca ccgagctgat cctcgccgac
     1561 ctgcagacca tcgaaaacgc gctgcccaag ctggagaagg atctgagggg caagaagatc
     1621 gagccggcct acatggacgc agtcaagcag gcaaagacca tccttgaagc cggcgagacg
     1681 ctcgacaagg ccgcacgcga aggccgcttc gacaaggatt ccgtctacga cctgcatctg
     1741 atgagcgcca agccgttcat ctacgtgttc aacgtggacg acaacgagct ggccaacaag
     1801 gacctgcagg ctaagctcgc cgcatccgtg gccccggctc cggccgtgtt cctcaacgct
     1861 cagttcgagg cggacctgac cgagctcgac gaggcggatg cccgcgaaat gctggccgac
     1921 gctggcttga gcgagtccgg tctcgaccag ctggcccgcg tgggcttcga catcctcggc
     1981 ctgcagacct tcctgaccgc aggcgtcaag gaagtgcgcg cctggcagat ccacaagggt
     2041 tggaccgccc cgcaggccgc cggcgtgatt cacaccgact tcgaaaaggg cttcatcaag
     2101 gccgacatcg tctcctacga cgatttcgtg gccgccgacg gctccatggc caagatcaag
     2161 gaggaaggca agctccgtca ggagggccgc gactacgtca tggccgacgg agatattgtg
     2221 gagttcaagt tcaatgtgtc caagtagggt gtgaacaccc cagaaaacgg cgtgattcca
     2281 acgttttcga gttttcggaa ctgtggaatt gcgccgtttt tcgattattg aagaatggcc
     2341 gattatgccc cgtatctggc cgtttgtggc ccggatagtg tgccaataga gtgccctttt
     2401 tgagggcgct tgtagcggat gctcgaaggc gctcccgaca agggaggaac atcatggcga
     2461 cgaacggcag acgacgcacg aagggtgcgg gaagcatcat ccaccgcaag gatggctcgt
     2521 gggagttccg gcgtgaggtc ggccgcgacc ccgccacggg caaacgacgc tatgtctcgg
     2581 ccaagggtcg cacgaaggcc gatgcccgcg aacggttcga cgcgaaggtc gccgagatgg
     2641 aacgcaccgg cctgctgccc ggcgccaaga gcccgtacct gaaggattac gcggagcggt
     2701 ggctggagga ataccggctc aacgtcaagc ccaccacgta ccgcacccgc gccggcagga
     2761 ttcatgcgtg catggaggtc atcggctgta tccgcctcac cgagctgacc cctgaccata
     2821 tccgccaatg catgcgcgtg ctttccaaac ggctcgcgcc ctccacgctc aaggaccatt
     2881 tcgtgagcct gaagatgatg ctcgaccagg cggaactcga ggagctcatc cccgtggatc
     2941 cgtgccgcag ggtcaagccg ccaagggtgg agnnnnnnnn nngagcccac cgagaccagg
     3001 atcctctcgc cagaccagcc gaagcagctg atcgaggccg tgcccaaccg gggagcgaaa
     3061 cgacgcggac cggccctgac cgtggacgtt gacgaatcct ggatgctgct gttcgaactc
     3121 gcgttcgcgg ccggcatgag ggaaggcgaa cgctacgcgc tcatgcccta cgagctcgaa
     3181 caacgcgacg gcgtgcccgg catcaacgtg cagcagcaga tccagcaata cggcaaaccg
     3241 gaggacgccg tgatacccgc ctggctcaaa gccgagcacc tgtacggtat cctgtggctc
     3301 accacaccca aaacccacgc cgcccaccgg ttcgtaccca tatccacgag cctgtggcaa
     3361 cgattgtggg cgcgcatcaa acgactcgac ataggcccac gcgacctcat attcaccaac
     3421 tcacgaggca accccgtgcg cagcagcacc gaacgctaca actggaacaa ggccttgaaa
     3481 gccgccggcc gcccgccggt caccatccac agcgcacgcc actggaccgc cagcatgacc
     3541 gcccgcgcca acatgcccga cgacgccaga accgccatca tgggccacac cagcatcagc
     3601 atgaccaacc actacaccca cagggacgcg gccagtctcg ccgcgctcct cgaccgagcc
     3661 atccctgacc tgcacgacga gagggatgtg atagacgcgc aggtcatcga ggaggggaaa
     3721 tgacgaacgt gacgcatagt agacttgtcc gcatgagcat aaagaaagta gacgggccac
     3781 aggaccatca tcgatttatc gatgaggccg gtgacatgac cttccattcc gggaagcggg
     3841 gccgcaaaac ctcctccatc gggatggacg gagtttcccg atgcttcatg atcgggctcg
     3901 tacatgtgaa aagtcccctc gacgatgctc gtgcgactat cgacggtttt tgtgaggaga
     3961 tcaacagcag caaattcttc cagagctttc ccagcgtcca aaagagaaca aaggagggat
     4021 ggcacggctt ctacccgcat gccagcaaag atcctgcgga actacgctat gaattcctca
     4081 aactcatggc gcaggagatc gacttctccg cacgagtagt tgtgggccgt aagataccgg
     4141 cgatctatca gcagaggcac aacgaacaac cacgtgaatt ctacgccgac ctcatgtccc
     4201 atctactcaa atactcaggg aagatagatc cgttgattct cgacgtcgcg gaacgaggca
     4261 gcagcacatc gaacctcaac cttcagcatg ccgtggacat cgcgcaacag cgcagccgtc
     4321 gcggcacggt aacgcatgaa ttgcgaaaca ccaccaaatt caacgtccaa ccgtatgatc
     4381 acgaaattct cttggcgctc gccgactaca gtctatggac ggtccagcga gtgtacgaga
     4441 agggagacga aaagttcttc gggcttcttg aaccgaagat tgtgctggtc catgatgttt
     4501 atgattccag cagatatttc aaatcaaaga actattatcg gccgaaatac aatcctctga
     4561 ccagagcatc catcaacttc gacggatggg cgcaacctga atggtagaag gaatagatgg
     4621 cccgtctcaa ctctagtcgt gctgtgacga cgacggcatg aagccgactt tcgatgaagg
     4681 acaggccaaa atccaataat acgaccagct gatccttcga ggggaaggat acctgtgtat
     4741 tcttgtcgct gatctataaa tgatgataga cggtcatgtg ggcggcagtc aagaaaatgc
     4801 gacagattgt ctaatcgtcg atatgtcgca gggccgcccc ggcgctcgca aagagaccgg
     4861 ggcggtttcg tgtttagggc ttgatccgcc cgacacggga tttattgctt ggccgtcttg
     4921 acggtgatgg tggtaccgag cgcggtggtc tcccagctca cgccgtccgc ctcactgtag
     4981 gtgaagtcct tggtggcatc ctgcgaggcg aggagcgcgc tggccatggt ctcggtgtcg
     5041 ccctggctgg tccatttcca atcgccggct gcggccggtg cctgataggt gcccttccag
     5101 tagaggctct tggtgtcggt gccgatccag tcgacctcga tggtgtcgcc gctgatggta
     5161 gcttccatgt atgagctcgg gtcgttggaa ttggtctgct tccatgtgcc ggtcagatcg
     5221 gccggctggg gtttgggctc ttccttattg gtctcggtct ttgacgtgcc atcggatttg
     5281 gccggcgcat cggccgtagt gcttcccccg caagcggaca ggccggtaag caacatcgcc
     5341 gcaacaagca gcgcgattgc ctttttcatg attggtctcc cttcgtgccg gacattgtcg
     5401 tccgacatga ctcaatcata tggcaaaaca caagaaccaa ggactcatcc cggtgctcgc
     5461 ggtgagtcgt cgggatgagt ccttatatgg ctcggaagct acgcggcgag ttccacggga
     5521 gcccgctctt ccaatttgga gtaggggata acggcacacc gtagttgcgg aacgcggtga
     5581 gcgcgccgcg ctgcaacggc gtctgccggt cgtcgccgat gactatcagc cgggaatccc
     5641 tgcgcttggg atccttcttc gtgtcatccc aaccgaacat gatgtctttc acggcgtcct
     5701 tgtcgaatcg gttcggagcc tggcagaacc gggtcggatg gttagcgcta cgctggaaca
     5761 ggaaatcgaa gttgtgctcg tagctggata cgcctcggat ccccacgttc ggagtgtaga
     5821 acacatggca tgagtcgagt ttcaatgcaa cgtcgtcagc aaagtaggac agcaccctct
     5881 tctgttctgt aacggcatca gtacaatgtt cgtaatgaac agttgttcgc gcaaggaaat
     5941 gccggcatat ggtgcgtcat gttcaaagca tagagattca ttatccggcc tccacgagaa
     6001 tgatttggtt ctagcgcaat acatgctttc acgacacatg ctttcgtgat acatacgttg
     6061 gtcagaagat attgcgctag aactcagcag tcaccgcacg tggtccacga tgtcgttgac
     6121 cttggtcata tcgatatcgc gctcgccgaa tgcctgccgc tcgtaggcca ggtcgatgtg
     6181 gtgttcgtgt gcgtaatcga tgatacggcg agccaaatcg gcaatgtcgc cggacttgtt
     6241 gaaggaacga ccacgcagca gcacgcgttg cataagaggg cgcatcgcct tgaccaactt
     6301 gtaccggtct gacttcacct tcaccatcca accgtcggca aaatatagca ccacgccttc
     6361 atgcgggctt gccttggcgt cgccgattgc ctgggcgact tcttccggcg aatggcagat
     6421 cgccaccgtt tccggccgca cgaagccgcc ggcaagagtg aacgtttcct cgtgagcggc
     6481 atcgatggag aacgattcgg cattacgaat caaatgcagc aagcgcagcc cggatgattc
     6541 gtatccaacg atgtgccggt cggactcgcg atcgatgact tcaaacgccg ccgtgacatt
     6601 ccactcatgc aacatacgcc acaattcggc acgtaccgct gaatctggag gaaacaggcg
     6661 ctcgataagc gcggaataat ccgtttgccc cgacttcgac cagaagcgga acaatccagg
     6721 agttcccgcc gcgcccacga gcccgagaaa tccgttctcc ttgcgctcaa cgcgcacggg
     6781 caacgactct ggatgttctt gagcgtgatt gacgacttgg gcgaacgatg tctcctccgt
     6841 ctcgtccaca gcaaagaact tctcgaaccc gcgttgcacc acctgaccgt tgccgccgac
     6901 aaaaagccct cgcgcattga tggtgcgatc cgtccaccga tgttcacgaa atgcctctgc
     6961 agtgaaattg cacgcataca cgtctgtttc gccgcgcacc ggaataaccc gaacgtaagg
     7021 gtccgcgcgc atacgctcca acaggtttcc attcggatcg cccggttcca acgggcggaa
     7081 catcgcggtg tgaaaatgct tccaggacga acgaatgtaa tcttccggaa cacggtcagc
     7141 ggtatcacgc gccgcattnn nnnnnnnntt gcgacgcaac aattcgtcta gaggaacgtc
     7201 aaaatccctc gtttcccaca gtgcgccatg acgttgagcg agtagcgcca gttcggctgc
     7261 ataccgggga ttagcgtgtt gcgcatccgc cactacatcc caccccgcat ccaatgcgtc
     7321 ggcaatcatg tgccgtccca tggcaaccac catgtcattg aatcgcttgc cgtgctcgga
     7381 ttgcagtcgg tttcggacct gtgccgagcc ggcgatcatg agccgaatgt catcgagaga
     7441 aatcacgcaa tgcggccacg tgttggcggg gtcctcacac catgagcgtg cccacgtgga
     7501 cttgcccgaa gcgggcagtc ctcgcagaat aatgagcttt gccatagcca agctcctttc
     7561 gttgtgctgt ggggcgagac aggtgccagc gatggcataa ggttctgctc gccccaatat
     7621 tgcttgtatt atgattcggc tgccgaaacc gagccgcctg gttcctgctg tgcggcggag
     7681 tcaatgggcg ccggcccggg ggagtcgggc atatccagcg gtgatacgtg cgttgaatcg
     7741 gtcgcgtgtg catggaacca gtcggcaatc tgtgtttcgc ttaccggttg cagattccat
     7801 gcgtccagtc caacgttgag ggcttcgtgg cgtgcgccct ccggagacag ctggtgcgtg
     7861 tgaccataca gcagacgatg tccgtcgaac ggcaatgcct gatcgcggaa acgtacggca
     7921 tcatccggcc agccataggc aagatcctca cggtagggga aatgcgatag attgacggtc
     7981 tccgttgcct gcgggcggcc aattccgaga ccggtcagct ccgccgtgct gttcggctca
     8041 atcgattcga acagatctcg cgcgggtgca tcgtccagcc accagtcatc gtgattgccg
     8101 ataaccgcgt gcagatgcct gcagcgcagc tgacgcaggc agctcttcag atgccggacc
     8161 gatgttcggt atccgatatc gcccaaaatc cacagctcgt cgtcctcgcc gacaatccga
     8221 ttgatattgg ccacgattgc ttcatcgnnn nnnnnnntgc ggccttgcgg aaattgagcc
     8281 gggaatcgtc aagcaccacg cccttcgccc aatcctcggc ggccttgcgt ccctgcgagg
     8341 agaggagagc gtcatactgc gtgtgcccag ggtcgaacgt ggtgaatccg cgcagcacgc
     8401 tgaccagcgg atgcccgaaa tgcgtgtcag tagtgaaata cttcatgatc tgttgttctt
     8461 tctgaatatg tgatggtatg caatgggttt tgctgcggtg cattgagttg cgactatgcc
     8521 aagaacgcat gctcgtgcag gtgcgtttat gaggcatggt gcgaacgagt cggctcgata
     8581 tcaccggcaa tgattcagct atgcgccaat gagaggtgta ctgatcagat ttgtatggca
     8641 gcgagcaggc tgcgcttgtt tccggttatt ggctggaact gagcagtgcg caaaccctgt
     8701 ttgaaggcgc attcgagcat cgccggcagg ttcatctgct gacgcttggc gtgttcgttc
     8761 acgccgttgc tgctgaatgc gatgtgttga atcatggtgt gcacaccata aggtcttacg
     8821 tccgaatccg tcaaatcacg gtgtgtgaaa tgcctgttgt atataaaaaa gtccggtgca
     8881 acgcacgatg ataatgcgct gcaccggacc tactgcag
//
LOCUS       DAWBMW010000056         8790 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_1910_length_8790_cov_2.715627, whole genome shotgun sequence.
ACCESSION   DAWBMW010000056 DAWBMW010000000
VERSION     DAWBMW010000056.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 8790)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8790)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8790
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1910_length_8790_cov_2.715627"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2626..2635
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7131..7140
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7931..7940
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1619 a         2548 c         2783 g         1810 t
ORIGIN      
        1 ggctacggca atcggcgaga acgcgccagg cagaaacgcc gccgacaaac gattcagcgg
       61 ggacggcagg tcgagaccga gctcatcaag ctggtcggtg gatgcgagca gtacctgcaa
      121 cgccttatag cgcgggcgac gcttgagccg gtagatacgg tcgatagcgg ccttattacg
      181 cggatcgcac gccactccat ataccgtatc cgttggaatg acgaccaatc caccatcccg
      241 gatgacacgc gccgcctgtg ccagcgattc atccgtgact ttcagcgttg ttccgccttc
      301 cacgctcacg ccacctccgt tcgcttgtgt attcacaatc taccattgca ccattgcgcc
      361 ccgacttgca tagttcatca tcgctcgttt gccgtactgc cgttttccga ttcgccccac
      421 acgcacagac cgtagataca atgagtccat ccatgctctt tcgcgaaatt acaggagtgt
      481 tcaatgagcg aaatcaatgc tgaatccacc accatgcctc ccgtcgaact gcgtatgccg
      541 cgcgccagcg agcgcgagga gatgctcgcc ggcaaactgt acaattcggt agaccctgaa
      601 ttgaatgccc tgcgcgagaa ggccggcgac ctgtgcgcgc gcttcaacgc cgcgtcgcgt
      661 accgatcatg ccgcccgtgc cgcgattctc gatgagctgc tgcccggcca cggtgccggc
      721 cttgacgtga tggggccgat tttcttcgac tatggctgca acatcaccat cggagagcgc
      781 gtgttcgcca acttcaactt cactgtgctg gattgttgcc cggtgacgat tggcgacgac
      841 gtgctgttcg gcccgaacgt ttcgctgctg ccaccgatgc atccgttgcg ttggcaggat
      901 cgtaacgtga ggcaggcacc tgatggttcc gcatacgact gcgagtatgg caagccgatt
      961 gtcatcggct cgaactgctg gtttggcggc aatgtgacgg taatcggcgg cgtgacgatc
     1021 ggcgagggtt gcgtgatcgg tgccggctcg gtggtgacgc gcgatattcc gccgcacacc
     1081 gtggccgtgg gtaatccggc ccgtccgatt cgcgagatta cggataagga tgcttccgcg
     1141 ctacagtatt acgcgcagtg atatatgaat gctcccgctg gcgagctgtc agcggagctg
     1201 actgagggtg gttgccggaa atctccgata accaccctca gcctcacttc gtgaggtagc
     1261 tcccgccagc gggagcatag taatgtgaga cgtcacgcgc cgaggtacgc cttgcgcacc
     1321 ttctcgtcgt gcagcaggtc ggaggccttg ccctccatgg tgatcttgcc ggtctcgagc
     1381 acgtaggcgc gatccgcgat ggacagggcc atcttcgcgt tctgctccac gagcagcacg
     1441 gtgatgccac gcttgcgcag cgtgacgatg atgtcgaaga tctccttgac cagcagcggg
     1501 gacagaccca tcgacggctc atccatgagg atggtcttcg gatggctcat cagggcgcgg
     1561 cccatggcca ccatctgctg ctcaccgccg gaaagcgtgc cggccagctg gcggcgacgt
     1621 tccttcaaac gcgggaagta gtcgaacacc atctcaagat ccttacccag actggactgg
     1681 tctttgagac tgaacgcgcc catctctagg ttctcctgca cgctcatgcg ggtgaacacg
     1741 tggcggcctt ccggcacatg ggccatgccc agcgtgacga tcttgttcgc atgcgttttg
     1801 agcagatcgt ggccatcata ggtaatgctg ccggatttgg caggcaccag accggtgatg
     1861 gtgtgcaggg tggtggtctt gccggcgccg ttggctccga tcagggagac gatctcgccg
     1921 tcgttcacct tgaggctaat gcccttgacc gcgtcgatcg cgccatagga aacgcataga
     1981 tccttgattt ccagcatcag cgcgctcctt ccgtcagtgt cttggcaatg cttggctccg
     2041 gttccgcctt ggtggtggaa tcgccgtccg cgtcatcgtt gttgtcgctg cccaagtagg
     2101 cggagatgac ctgcgggttg ttggcgatct cctccggcgt gccggatgcg atggtgcggc
     2161 catagtccag cacctggatg cgctggcata cgctcatcac caggctcatg tcatgttcga
     2221 tgagcaggat ggcgataccg aaacggtcgc ggatcgtgtt gatgcagtgc agcagatcct
     2281 cggtttcggt ggggttcatg ccggcggccg gctcgtcgag cagcagcagt ttcatgccgg
     2341 tggccagggc gcgtgcgatc tcgagcttgc gctgctggcc gtacggcagg ttggccgcct
     2401 gggtggtggc cagtccctcg aggttgaaga tgcgcagcag gtcgatggcc ttctcgtgca
     2461 tctcgttctc ctgcttccag aagcccgggg tgcggaagat ggcgctgccc atgtggtagg
     2521 tgaacgactc atcgaaggcg acgagcacgt tctcctcgac ggtgagctgc ttgaacaggc
     2581 ggatgttctg gaaggtccgg gcgatgccgg cacgcacgac ctgatnnnnn nnnnncgacc
     2641 tgataggtct tcttgccgtt catccgggtt ccgtccagcc agaactcgcc ttcggtgggc
     2701 ttgtacacgc cggtcagcag gttgaacacc gttgtcttgc cggcgccgtt ggggccgatc
     2761 aggccaatga gttcgccctt gttgatggtc atgttgaagt tggacacggc tttcagacca
     2821 ccgaaggtga tgccgagatg ttcggctttg aggatgggct gggttgcgtc gctcatcgtg
     2881 aggcctcctt cacggtcttg tcgtcggact ggtcatgctg cttggcgctg gccgcgggtt
     2941 tcgcgccctt gccggcggcg ccgcggaaca gcatgcggtt gaccagacgc ggcagtgaga
     3001 actcccagga tccgaagatg ccctgcgggc ggaagatcat gacgagcacc agcaccaccg
     3061 agtaggcgag catacggtag tcggcgaagg cgcgcagcag ctcgggcagg atggtcaggc
     3121 cgatggcgga gacgatcgag ccggtgagcg aacccatgcc gccgaacacc accatgatca
     3181 cgtagttgat ggagttgagc caaccggcca tggtggggtt gagcgagccg atgtactggg
     3241 cgaagatacc gccggcgatg cccgcgaaga acgcggagat cgcgaagacg agcaccttga
     3301 ggtacgtggt gttgagtccg gaggagccgg cggcgatctc gtcgtcgcgg atggccttga
     3361 ccgcacggcc gtaacgggag cggccgaaca tgaagagtac gaccacggtg accaccatga
     3421 tccagaacac gacgtacagg ttggcggtgc gttcgatgcc gatcagcgcc tgtccggcgg
     3481 cgcccttgtc gaggcccatg ccgccggcta ccttgaggtt ctggatgatc acgcggatga
     3541 tctcaccgaa gcccagcgtg atgatggcca ggtagtcgcc gtgcagtcgc agggcaggaa
     3601 tgccgatcag gatgccgaac acgcaggcca ccacgccgcc gatcagcgtg gtgagcagga
     3661 agcgggcggt ggggtcgatg gcaatgccgg cggcgaccat gtatttggag gtcagtgcgg
     3721 ccacgtaggc gccgatggcc tcgaaaccgc agcagcccag cgtcagctgg ccgagcacgc
     3781 cgatggtgag gttcagggag gtggtcatga tgatggcgat gcacgcggtc atcatgatgc
     3841 ccttgatgta ggagtcggcc aagccggatt cgcacagcgc catcacgatg ccgaacgcgg
     3901 cgaggatgcc gatgaagcac accagatagg actgtttggt ggaaagcatt gtcttcttca
     3961 tctgatcaga ccttctcccc ttcgttcttg cccataatgc cggacggttt gacgagcagc
     4021 acgatgatca ggatggcgaa cacgatggcg tcggagaacg ccgaggtgat gatgcccgcc
     4081 gtgatggtgc cgatatacgc cttggtcagg ctttcgatca gaccgatggc cagaccaccg
     4141 atcatggcgc ccgggatgga accaatgccg ccgagcacgg cggcgacgaa cgccttcagg
     4201 cccagcatgg cgcccatcat aggcgagacc tgcgggtagg cggcgcagta catcagcgag
     4261 gcgaccgcgg ccagaccgga gccgatggcg aatgtcaggg cgatggtgga gttcacgctg
     4321 atacccatga gcacggcggc ctccttgtct tcggagacgg cgcgcatggc cttgccctgc
     4381 ttggtgaatt tgacgaacag ctgcaggccc accatgatga ccaggccgat gacgatggtc
     4441 agcagggtgt ctcccggcag cttgagcttg ccgaacgcga tggacggcag gtggaagatg
     4501 gtcgggacgg tctggaagtc ggcgccgaag atggcctgtg agccgttctc cagcagcaac
     4561 gacatggcga tggcggtgat cagggcggtc atggagttgc ccttttcacg caccggctta
     4621 taggcggcct tttcgacgat cacgccgacg gccacgcata ccaggatggc gggaatgacc
     4681 gccacccagg cgggcagtcc catggccacg atggccggaa tggtgagcat gagcacgtag
     4741 gctccgacca tgatgaaatc gccgtgggca aagttgatca gtcggatgat gccgtagacc
     4801 atggtgtagc ccaatgccac cagcgcgtac acgctgccga ttttcagacc gttgaacagc
     4861 tggctgatga acatgatgat ctgatcggtc atagctcccc ctttctatca atgttgtggt
     4921 tctctaccgc tgcgcgcgta gagatattcg tatgtcttgc gattgatggc gcgatacgcc
     4981 cgcgcctggc gatatccgca tatcgacggc tgtccttatg catccgagcc catgaaaaca
     5041 cgaaagggag aggaccggca aaaccggtcg ctctcctgtt catgcggaag tcatgctagg
     5101 acatcgtgat tatgcggtgg gaattcatgg aattcacttg ggctgcacgg tggtgttgta
     5161 caccggcttg ccgtccttca tttccagcac ggtcacggac ttggtcggcg agccggactt
     5221 gtccatggtg aacttgccgg tcacaccgtc gaaggtcatg ccggccacgg cgttgcgcac
     5281 atcctcacgg gtggcgttct cgccagcctt ctcgatggcc tgcttgatca tgtagatggt
     5341 gtcgtagccc agagcggcga acgtgttcgg ctcggcgttg ttcttggact tgtaggactt
     5401 gatgaagtcc tgcaccttgg tgttggtgtt gtcgtcggcg gagtagtggg tggtgtagta
     5461 ggtcttgttg tactgggact tgtcaccggt gagcttgagg ccatcgtatc cgtcgggtcc
     5521 catcacgtag ccctcgaaac ccacggagcg ggcctgcggg atgatgtacg ggccagccac
     5581 ggagtagtag tacggagcgt agagcagctc ggcgccggaa gcctggatct tctgcagctg
     5641 ggcggagtac tcggtgtcgt cggcggagga ggagctttcc ttgtccacca cttcgaggcc
     5701 gagcttttcg gcggccttgg cgaaggcctc tcccacgccg gaggagtacg gatcgccggt
     5761 gccgtagagc acggccacct tcttgacctt caggttctcg gcggcgaagc gtgcagccac
     5821 ctcaccctgg taggagtcct tgaagcaggt gcggaacagg taattgccgg tttcgatgga
     5881 gtcggaggtg gcggccgggg cgatggtgac cagcttggac tggtcggcca gcggggccac
     5941 ggctgcggtg gtggcggaaa tggtcgggcc ggccacggcg agcacgttgt tgtcgccggc
     6001 gagcttgttg taggcgttgg aggcttcggt ggcgtcgccc ttgtcgtcca tggactcgag
     6061 cttgaccttc ttgccgttga tgccgccctt ggcgttgatc tcggagaccg ccagctcgaa
     6121 gcccttgacc tcggcctcgc cgtaagcggc ggcggtaccg gagttggtgg tcacggtgcc
     6181 gatggtgatg gagtcgccgg aggccttggc actggagctg gaggaatcat ccatgctgcc
     6241 gccggagcag cccgcgaggg acaacgccat aacagcggcc gcgctggccg caacgaactt
     6301 agtggagaac ttcacgttca tcctcttcct tcttccttgg gatatttcaa catcgttcat
     6361 ctgtcaacca acgcgcggga tgtgcgcgtg agacgttcca ctcacataat tcgcatacca
     6421 cagaactaca tcacacggct tgtgaccgtc attcacgtct ctctcatccg atgtttgtcg
     6481 cggactgtta taaccagacc ggctttgaac ccgcaataaa ttgttcttat tatgagacag
     6541 cgcaattacg gaaacgagaa tacaatccca aggaaatgtg ggattcgtgg ggcttaggac
     6601 actgcaaaca ggtagcggtc gcgacccgtc cagtcctggc cggtcgatgc ggcagcgaat
     6661 ccggtggccc tggcaaaggc cacgagacga tccccctggg tcacgtcatg ttccatgaca
     6721 agcacgcccc caggcttgag aagacggcag gcccgctcga taatgcgctc gggaatcaac
     6781 gtaccgtcca tcgagccgcc atacaaagcc aattccggat cccaatcacg cacttccggc
     6841 tgctcgggaa tatcggtctg cggcacgtag gggggattgg tgatgacgat atcgacggtg
     6901 ccgtccaatt gggccaatgt ggcgaaggac gtggcatccg cgatttccag atggtaattc
     6961 gaggcaatgg atgggtactt tttcgcggtt tccgacagat tccttcgcgt ccattcggcg
     7021 gtgttcggcg acagctccac cgcccacacc tggctgccgg gcacctcgct gacaacggac
     7081 agcccaatcg cgccggagcc ggcgcacaaa tcgaccacac aaggatggat nnnnnnnnnn
     7141 atcatgccgt tttttgtcag ccagtcaagg ccggcctgca ccacggtttc ggtttccgga
     7201 cgaggaatga acactccggg accgaccttc aggtcaaggt agcggaaagg agcatgcccg
     7261 gtgatgtatt gcagcggctc gcgcttggca cggcggtcga gcatggcacg gaagacggca
     7321 agctgtccgg ttgtgccaag ttcctccccc ataagcagag ccctgtcgac gtcgcgcagt
     7381 tcaacgcctg ccgcctcggc aaggagcaac ttggcatcat gctcaggcgt ctcaataccc
     7441 gcttcgcgca attgcgaaga ggcttggttg atgatgtcgg taataagcac tggcggtctc
     7501 cgttgatcgg acattaggct cccctcagtc agcttcgctg acagctcccc tcacagaggg
     7561 gagccatccg gcaaagtcat ttctgattgg ccagacggtc ggcctcgtcg gcttggatgt
     7621 cggaatcgat gacggcctgc aggtcgccgt cgagcacggc gtcaaggttg taagccttgt
     7681 aattcgtgcg atggtcgacg atacggtttt ccgggaagtt gtatgtgcgg attcgctcgg
     7741 agcgatcaag cgaacgtacc tgcgagtgac gcatgtcggc ggcctcggcg gcctcctgct
     7801 catgcttcat ggccagcagg cgggacttca gtacgcgcaa cgcggcggca cggttctgaa
     7861 tctgcgactt ctcatcctgc atgttcaccg tgattccggt tggcaggtgg gtcatgcgca
     7921 cggcggaata nnnnnnnnnn gaatacgtgg tgttcacgga ctggccgccg gggccggagc
     7981 tcatgaagat gtcgattttc aggtctttcg gatcgatctc gatctcgtcg tcatcctcgt
     8041 cggcctcggg gaacacgatg acgccggcag cggaagtctg gatacggccc tgcgattcgg
     8101 tgacggggat gcgctgcaca cggtgtacgc cgccctcgta cttcatcgaa gcccacacgc
     8161 cgtcttcggg agcgggcgtg cccttggcgc ggatggcgat ctgcacgtcc ttgacgccgc
     8221 cgagctcggt ggtgttctcg gactgcacgt tcacgctcca gccacgcttc tcggcgtaac
     8281 gggtgtacat gcgcagcagg tcgccggcga acagtgcggc ttcctcgccg ccggtgccgg
     8341 ccttgatctc catgatggtg tcgcgcgcat catccgggtc acgcgggatc agcgcggtgc
     8401 gcagcttctc ctcgacaccg ggcagttcgt cttcaagacg cttggcttcc tcggcgaagt
     8461 cggcgtcctc gccggccatc tcctgagcgg ctgccagatc gtccttcacc tgcaaccagg
     8521 ctttgtaggc acccacgatg gcacccagtt cggcgtgacg acgacccagc ttgcgcagct
     8581 tgtccgggtt ggacacgact tcggggcttg ccatctgttc ttcgatggac tggtactcct
     8641 caagcgcggt tgccgccgcc gggaactgtt cgtctgccat aacacacttt cttctgtatg
     8701 gtgctgttct gttcgattat tcgattgcgc aggtcaatgc tgcaacaaaa cttgtcaaag
     8761 aatacaaaaa acgccagtcc tcgatcgggt
//
LOCUS       DAWBMW010000057         8721 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_1940_length_8721_cov_2.454073, whole genome shotgun sequence.
ACCESSION   DAWBMW010000057 DAWBMW010000000
VERSION     DAWBMW010000057.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 8721)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8721)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8721
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1940_length_8721_cov_2.454073"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2042..2051
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7082..7091
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1729 a         2772 c         2527 g         1673 t
ORIGIN      
        1 cgcgcgcatt cagatcctcg acaccttcgc cgccatccgc gcggcccggc cgagcaccgc
       61 catcgtcatg gtctcccacg atctcggcgt ggtacagcac atcgccgacc gcatcctcgt
      121 gctccatgac gggcgtctcg aagagacggg ttccaccgct gaagtgctcg gcaaccccca
      181 atccgattac acccgtcagc tcatcgaggc agcctcgtta tagacctttt ttggctccct
      241 ctctgagggg agctgtcgaa tgcagtgagg ctgaggggag ttacgggtgc cgtcgtattc
      301 ccctcagtcc gcttcgcggc cagcccccct cagggagggg agccgaggcc gccggcgaca
      361 cgcgcttgtc gcgggttgtg ctatggtgtg agacatgcag attatctcat gttgttgtcg
      421 ctaaccgacg cccctgggcg agcggtcagt gacgcataac ttgagataga gcattcgaat
      481 cgcggcaaat ctgtcgcatg aattttcatg aaatcgatgg cacgagcgta accgtgtaac
      541 gcgcgagccg tggttaacat gaaaaaccga ttcactttcc ggcttcaccg tacagtgagc
      601 gggaatactc gatttcaaac taaagccaca gtccattcgt ctgggaacgt cggttcacga
      661 gcgcaacgca gcaaaacccc acaaccccag caacgaagga cttcacatga ccatccacaa
      721 cagcctcgca gaactcatcg gcaacacccc gctcgtcaag ctcaaccaca ttccgcaggc
      781 ggccggcgtc aaagccacca tcgccgtcaa agtcgaatac ttcaaccccg gtggatcttc
      841 caaggaccgc attgccgaac gcatcatcga cgccgccgaa cagtccggcc aactgaagcc
      901 cggcggcgtg atcgtcgaac ccacctccgg caacaccggc gtcggcctgg ccctggtcgc
      961 ccagcagcgc ggctaccgca cgattttcac cctgccggac aaggtctccg aatccaagcg
     1021 cgcggtgctg cgcgcctacg gtgccgaagt catcgtcacg ccaaccgacg ccggcccgga
     1081 cgacccgcgc tcctactatc aggtcgccga acgactggcc aatacgattc ccggcggctt
     1141 ccgcccgaac cagtacgaca atccgaacgg cccgctgagc cactactaca ccaccggacc
     1201 tgagatctgg gaggccacgg accacaaggt cacgcacttc gtggccggca tcggcaccgg
     1261 cggcaccatc tccggcaccg gcaagtacct caaggaagcc tccaacggcg cggtcaaggt
     1321 catcggctcc gacccggaag gctccatcta ctccgcttcc tcacgcgacg aagtccacca
     1381 gtacgatatc gagggcgtag gggaggactt ctatcccaag gccttcgacc ggaacatcac
     1441 cgacgacatc gtgcgcgtgt ccgacgccga agcctttgca atgacccgcc gtctggtcgg
     1501 cgaggaggcg ctgctggtcg gcggctcgtc cggcatggcc gtggccagcg ccatcaagta
     1561 cgcgcttgcc aatgatttgg acgagaacca gatcgtggtg gtgctcgccc ctgattccgg
     1621 ccgcagctac ttggagaaga tcttcaacga cgactggatg cgtgccaacg gctacggcga
     1681 catcgtggag cgcaccagca agccctccct tgccgagcag taccttaacg gagctccctc
     1741 tgatgaggga gctggggacg gactccataa ctagctatca aaagaatcaa taccaacatc
     1801 cgcactaacc aaccgcacct gttctacctt cgaatacgga acgatcccga aaggaaccca
     1861 atatcatgtc cgctgaatac aacgccaagt tcgccaacac cgacatcgcc actcgcgcca
     1921 tccacgccgg ccaggagccc gacccgacca ccggtgcggt cgtcacgccg atcttcgcca
     1981 cctccacttt caagcaggat ggcgtgttgg gcctcagggg aggccacgac tactctcgct
     2041 cnnnnnnnnn ncatcaaccc cactcgcacc agcttcgacg agcagctcgc cgccgtggaa
     2101 ggcggcaagt acgcgctgag cttctcctcg ggcttggccg ccatcgacgt gctgttgcgc
     2161 tccaccatca agcccggcga caacatcctg ctcggcaacg atgtgtacgg cggcacctac
     2221 cgcctgctct ccaaggtgtt cgtgccgtgg ggtgtgggcc tcgacgtggt ggacatcacc
     2281 gatctgaaag ccgtggaaac cgcattggcc tccaagcact accagtatgt gtggatcgag
     2341 accccgtcca acccgctgct caacatcacc gacatcgcgg tcaccaccga agtcgcccac
     2401 aagtacggca ccaaggtcgc tgtcgacaac accttcgcct cgcccgccct tcagcatccg
     2461 ctggccgatg gcgccgatgt ggtggtctac tccaccacca agtacatcgg cggccactcc
     2521 gacgtggtcg gcggtgccgt ggtggtcaac gacgaggaga cccgcgagaa ggtcgccttc
     2581 ctgcagaacg ccgccggcgc agtgccctcc ccgttcgact cctggctgga catccgcggc
     2641 ctgaagaccc tggatctgcg cgtcaagcgc cacagtgcca acgccctgaa ggtggccgaa
     2701 tggctcgaat cccagccctc tgatgtgatt gagcgcgtct ggtacccggg cctcgagtcc
     2761 cacccgggtc atgaaatcgc cgcccgccag atgcacggcg gcttcggcgg catcgtctcc
     2821 gtgcagctcg cggccggcgc cgaggcggcc aagcacttcg tggaccacac gcagatcttc
     2881 acactggccg aatcgctcgg cggtgtggaa agcctcatcg aggtgcccgc cgccatgacc
     2941 cacgcctccg tggccggcac caccctgcag gtgcccgcca acctggtgcg catttcggtc
     3001 ggtatcgaga acgccgacga ccttatcgcc gatctcaagc aggcgttgga tcgtatctga
     3061 ccgtactgac ggtactggtg gcaccgtatt gacggtgcca ccacgcttat cggcggactc
     3121 gctatcgatt ggtacctaca aaaaatcccc ggcccacaag ggtcggggat tttccgttat
     3181 cgattaaaca tattgaatca gctggcgttg ccgccctgcc atccagtggc atccgcacca
     3241 gccgggcgca acgtctgacc ggaagtcggt gtggtgccat caacgaccac acgagtgccg
     3301 atagtggcaa agtcgtacat ccacttggca tcggccacct gcatgttgat gcagccgtgc
     3361 gagcctggga taccctgctg cacatagatg gggtgctcgc cccacatgta tgcaggaaca
     3421 ccgtgcagag cctcgccgcc gttatagtag gaaacccacg gcgtgcctgg ctggttgtag
     3481 ccttcgccaa ccatggtctg cgattcgtac ttgagataca cgtagaacgt gccggtatcg
     3541 gacagcatgg aaccatcggc ggtgggcttg ccagaagcga tgttgaactt cttgaccacc
     3601 gtggtgccct gatatgcagt ggcagtctgg ttcgtcagat caacatgaac ccacttatcg
     3661 ccatttgccg tatcgaagtt atgaggcacc tcgacctgct tgacatcata cgggctgtct
     3721 ttcacagcca cagtggaatc agtggactgg ttctgctcga tggcggtagc cagacgttcg
     3781 ggggcgtccc cggcgccagt cacttcaata ccatccacgc cgagtgtctt atcggcacca
     3841 atttccacgc cgccttcgtc tttcggcgtg atgtagacct cgcgtgtcac cttgggaaca
     3901 gtcagttctt tggttacgct gtcactggtg acgtacttgg tgatggcatc gcggtcgact
     3961 accagactca tggtgccttc cgccttattc accgtgggct tgaggaatga ggcaatggta
     4021 tcggccggga tggtgacgct cttgttcacg ccgttgtcta cggtcagttt gaggccaagc
     4081 ctggcattgg cgtcaaactg tgtttgctgt gcgctcgcgt catcaatcgg attctttgcg
     4141 gtctgtagct tgaccggcac cgtcgcattt tcgcccgggg tcgcgacggc ctccttgact
     4201 gcagcattga caacattggt atccgggccc ttgccgtcct gacccgcggt gtagtcgaac
     4261 tgtttggtgc tcttgttgta gacaacggtg gcaatctgcg ctcggtcggc ctcatcgacc
     4321 aggccggcgg tgacgaattg ttcggcagtc tctttgtccg tggtggcggt cagagcgata
     4381 tgcggctgat cgaaaatatt gagcttggcc acgtcgccgg tcttcgcgtt caacagcgca
     4441 tctacggttt tatccgtgtc gacggtgacg ccaaggtcct tcagcgaggc tttgacgctg
     4501 ttgccttcgg cggtgaacgt cactgtggtg ttattcagct gctgcttcac agtattcgca
     4561 agttcttcgc gggtctggcc catgacagag acattgccca aatgaacacc gggcgcagcc
     4621 ttgtcttgga agtaccaacg tccgacgaag aaataggcta ttaatgcggc aagcaacact
     4681 gcaaatatgg ttatgatggc aatccgtgcg gccttgccat gtgatttagc ggtggtagat
     4741 gccatgacat gttcacccac ataggctgta ttgtcggcat ggttattggt gccattgtcg
     4801 ctttgctgtg gcgtggcggt acctgtggcc gcggctgcga acagcccatc ggcggccaca
     4861 ggctgcagca ccgccgtctc ctgatcgtca ggagaggaga aggaaaatgt atcgttggcc
     4921 ataaaatgcc ccgtcttagt tctctcaaag cggcatatcg tgtgatatgc cgggaatagc
     4981 gttcctcgac gcataataat acggaagggc tacgtacatc gttacgaggt attccgttat
     5041 acgatgaaat ccatccgcgg tcggcctaca ctggtcgttt atgacagcac atcaggcggc
     5101 gctggaggcg ctcacacgat acttcggcta tgactcgttt aggcccggac agcaaggaat
     5161 tgtcgaggct ttgcttgccg gacgcgatgt cttgggcgtg atgcccaccg gcgcaggcaa
     5221 gtccgtgtgc taccagattc ccgccgcgct cagcccgggt atgactttgg tcatatctcc
     5281 cttgatttcc ctgatgcgcg accaggtcga tgcgctgaac gacctcggtc tgccggccgc
     5341 gttcatcaac accactcaaa ctcccgacga gcaggcgatg gtgttcgccc aagccgccgc
     5401 cgggcagatc aaactgctgt acgtggcccc ggaacgcttg gaaaccgggc gattccgcga
     5461 ctttgccgcc cgtacgccga tctcgctgat cgccgtggac gaagcccact gcgtctccca
     5521 gtggggtcag gacttccgct cgtcgtactt aggtatcggc gatttcatcg ccgggctgcc
     5581 gcagcgtcca ccggtgggtg cgttcaccgc caccgccacc gaacgggtgc gccgggacat
     5641 cgtgggactg ctgggattgc ggaatccggc cgtcaccgtt accggattcg accggccgaa
     5701 cctgtatttt gatgtggtca aactggaaac caaatacaag gccgcatggg tggcgcggta
     5761 tgtggccgac catccggacg aatccggcat cgtctactgc gccacccgca agaccaccga
     5821 agcgctcacc gacacgctga accagatggg gcatccggct gtcgcctacc atggcggcat
     5881 gtcgccggac gcgcgtgagg cggcccagcg cgacttcatc accgacaagg tgccggtggt
     5941 cgtggccacc aacgccttcg gcatgggcat cgataagtcc aacgtgcgtt acgtcatcca
     6001 ccacaatctg cccgaatcca ttgaggccta ctatcaggag gccggccgcg cgggccgtga
     6061 cggcgagccc agccgatgca cgctgctgtg gaatgagtca gacatcgtca cccggcgtcg
     6121 gttgctggat aacgattatg agaacgaacg attgactccc gaagagcagg aaatcgttcg
     6181 ccagtccaag cgccggctgc tggacggtat ggtcggctac tgccgcacca ccgactgcct
     6241 gcaccggtat atgacccggt atttcggtca ggaattgtcg ccgaatgctg gttccacggc
     6301 gggtgaagac attgcggcgg actcttcgcc gtccggcaaa tgcggtgcct gctcgaactg
     6361 cgaaagcacg ttcgagacca tcgacgtgac ccgcgtggcg caggcgatca gccgttgcgt
     6421 gcatgacgtg gggcagcgcg tcggttccgg caagatcgtc aagatcctgc gcggttcgcg
     6481 ggcacaggat ctggcatggc tgaacccgga gcggatgccc accttcggca tgctcaagga
     6541 cgtgaacgag gctcgcgtgc gcgacgtgtt gagccagatg gccacggacg ggtacctgtc
     6601 aatcgccgaa gggcgtatgc caatcgtcat gttcggtgcg cgggcggccg aaaccgccgc
     6661 tcccgacttc cactatgaga tcaaacgggt ggaacgcaag tcggaggcgg ccggttccgg
     6721 tcggagcggg ggagtggctg atactgccga ttccgccaat gttcccggcg atgcgctggg
     6781 ctcgtatatc ccggacgatg acgaggaatc gctgttccag aagctacgcg aactgcgacg
     6841 caccatcgcc caggaaatcg gcaagccgcc gtacatcgtg ttctccgaca agaccttgcg
     6901 cgacatggcc cgcatcaagc cggttaccaa cgcgcaattt ctggcggtca acggcgtcgg
     6961 tcaacacaag ctcgacctct acggccagcg cttcatgcaa gccatcgcct cgttcaatgc
     7021 cgggagtgcg tcataacaat ctcctatagc gaactgcgga gtatttgcgg gcgttcttgg
     7081 gnnnnnnnnn nttcttgggc tggctcgact acgatgactg ttcgatgctt catgaccgaa
     7141 cactcggcaa gtagaaaggc aacagcatgg cagaagaaac ggccgaaaag cccgtcgtcg
     7201 aatcattcca gcttgatcac accaaggtca aggcgccgta cgtgcgttac atcgacaccg
     7261 aaacgggccc tcacggcgac gtgatctcca actatgatct gcgcctgacc cagccgaaca
     7321 agcaggccat ccccaccggc ggcctgcaca ccatcgaaca caccatcgcc gtgctgctgc
     7381 gcgaacgtat cccgggctac atcgactgct cgccgttcgg ctgccgcacc ggcttccacc
     7441 tgctgacctg gggcacccac cccaccgagg aagtggccaa ggccctgaag gaatcgttgg
     7501 agttcatcgc gtacaaggcc acgtgggatg acgtgcccgc caccaccgag aagagctgcg
     7561 gcaactatcg cgaccacagc ctgttcaccg ccaaggaatg ggccaagcag atccttgagg
     7621 aaggcatcag ctccgacccg ttcgagcgca aggtcgtctg atcgcaagcc aatcggcatg
     7681 gaagaacccc gcccccgcat accacgcgga cggggtttct ttgtgcctat gtttgtgcct
     7741 atggcatggt aggtttgccg tcagttaatc gttggccgct gcacatcggt agcttggcat
     7801 gttggagagg tggaagtgac tcagtcgaac gctgcaaaca aggcgacagg ccgccgtttc
     7861 cgccacgcca ccatgcgcga tctgccgcag atgcagaaaa tctacgcacg cgcacgcgcg
     7921 ttgatggccg ccaacggcaa ccccacccag tggggcaacg aatttccgcg tgaatcgatg
     7981 attgaggacg acatcgccaa ccagcgtacg atgctgctgg tcgacacctg caatggccac
     8041 gaacgcattc tggcccagtt cgccctgtgc ccgggcgagg atccgaccta cgccaaaatc
     8101 gacggcgcct ggctggacaa cgacgcctat gtgaccatcc accgcatcgc cgcatccggt
     8161 ctggtgcgcc acgcggcccg cgactgtatc aggtgggccc tgaagcacta cggcaacgtg
     8221 cgcgccgaca cccaccccaa taacaaggcc atgcagcacg tgctcgaaag ctccggcttc
     8281 gcccgttgcg gcctgatcca gctgctcgac cggccggtcg acaccaccag aatcgcctat
     8341 cagcgccacg agtggtgagt gtggctgccg gctgatgtgc tcgtttctgc accgtcatcg
     8401 gtttctgacg gtagaatcag tgtgtgagaa gaacacgttg ggtcgggggc gtcgtgacgg
     8461 tgcttgcggc tgtcatgctg tcgtcgtgcg gcggcgtcca gtcgaatccc gggtaacaca
     8521 ccgttgccaa tccattcacc gcgcaggtga atggagaaga catggcgatg cctgtcggcc
     8581 gatggagccc catcggccga caggcatcga ggaagtcacc gtggccggcg gaacgcgcgg
     8641 tcgccgattg gggcgaatgg cttgtgtggt tcgctttggt accagtaggc gaccgtggcc
     8701 acgtcttcct gtcgttcgaa g
//
LOCUS       DAWBMW010000058         8670 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_1952_length_8670_cov_2.653511, whole genome shotgun sequence.
ACCESSION   DAWBMW010000058 DAWBMW010000000
VERSION     DAWBMW010000058.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 8670)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8670)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8670
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1952_length_8670_cov_2.653511"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    885..894
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5111..5120
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8142..8151
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1854 a         2532 c         2545 g         1709 t
ORIGIN      
        1 tcccggccct gcattcgacc aatcgtgttg cgcgtgaggc cgctgaacgt gcggcactca
       61 acgcaccgat tcagggatcc gccgccgaca tcatgaagat tgcgatgatt cgcgccgagc
      121 agaccttggc cgaggctcac gtcaagagcc gtatcattct gcagattcac gatgaactgg
      181 tagtggaaat cgcgccggga gaaggcgacc aggtcaccga actggtgcgc aacgccatgg
      241 aacacgccgt cgaccttgcc gtgccgctgg acgtatcgtg cggcatcggt tccgactggc
      301 aactcgctgc acactgacaa tttctagctc ccctcgggtg agggggctga gccgttgagc
      361 aaacagtcca gtggattgtt tgtaggcgaa ggcgagccga tagtcgagca atgaagagca
      421 cgagcatggc ttgtccgcaa ttgcagaatg agtcccttgc agcaatagcc cactacaatg
      481 ggacatatgc cgaatctcaa gcaagtcatc gacgttctcg agaccctgta cccattgcgc
      541 tacgccgagg aatgggatga gccgggcctc attgtcggcg atctgagtca cgacgtgcac
      601 cgcatcgtgt tcgccgccga cccgacctcc gccatcattg acaaggccat cgctactggc
      661 gcggacctgc tcatcaccca tcacccgttg ttcttccgtt ccgtacacga gacgagcgga
      721 ctcggtttcc ggggcgacat cgtccgcaga ctctaccagc acggctgcgg gctgtgggta
      781 gggcacacta atgccgacgc tgcgtatcgc ggcgtgggac aggcggcggc cgactacttc
      841 ggactcatcg accagaaacc actcgtgccg attgacgatg caaannnnnn nnnngcttgg
      901 ggcgtgtggg tcgtttgccg aagcctgttg ccctgaagga tttcgctcag cgagtattcg
      961 acgaagtgtc cgatcatggc atgaccagcg cgctcggcat ccaggtatgc ggcgattcgg
     1021 acacttttgt tcagtatgtg gccattctgc ctggctccgg ggactccctg ttcaacgagg
     1081 tacgtgccac cggtgcggac gtgtacgtaa ccagcgattt gcgtcaccac ccggtgaccg
     1141 acgccatcga acaagcccgg tatgaagccc gtatgaggtc ccaaggcatc atgctcggcc
     1201 atggaatggc gggggagagc catgtgcgtc cctgcttcat caacacccct catgccgcca
     1261 tcgaatccat gtggttcaac tacgcgatcg atgacgtgcc agccgccatc gaacaagcga
     1321 ccggagcaag gcctgaagtc gagtggttcc acgacacgac cgatccatgg acgctgtcca
     1381 ttaccgcgca tcgagattaa gacgctatag taacgtggca aaaaacgttt tttccgagga
     1441 gatgacatgt ctggcggaag caagcaggaa caactggagc atcggctcga ccataagctg
     1501 aacgcctcct cggacggcat cgacatggac gtgcccgcca aagtgatcag tagcgaaacc
     1561 gtttataccg ggcgcatctt ccacgtggac gacatgcgca tcgctctgac cgacaagcag
     1621 ggcaaggaac acgagatcgg ccgccaggtg ctgcgtcacg ccccctgtgt ggtcatgctc
     1681 gtgcacgaca tgtccaccga ccgttacctc atcgaacgcg aataccgtgc cggatcggac
     1741 atgttcgcct acgggcttcc cgccggtctc atggacgagg gcgaggacat catggacgcg
     1801 gccctgaggg aactggccga ggaaaccggc gtggtgccgg accgtgacac catgggtgtt
     1861 gacttcgtgg gcgatttcta ctcgtccgaa ggcatgaccg acgaactggc ccacatcatg
     1921 gtgttgcatt tgggcccgtt caggcgggag cagcgccatt tcgacgcgga cgagcacgtg
     1981 gaatccgcgt ggattccgtg gagcgacctt gccgccacgc gcatcacctc atccaattcg
     2041 atgatcgcca tccagcatga ggcgttacgc agactcatcg tcaggaactc cgacaaagac
     2101 aagccgtcgc tgttctcctg acgttcaact ccccggcaca tcagtgacac aagaaagtgg
     2161 gatactgaaa actatgcaga tcagacctgg atcaatgtat ccgctgggcg ccagctatga
     2221 cggcgccggt gtgaacttcg cgctgtactc acaggttgcg cagaaagtcg agctatgcct
     2281 gttcgacgag cacgatgcgg aaacgcgcat cgaaatgacc gaacagaact catacgtatg
     2341 gcataactat atccccggcc tccagcccgg ccaacgctac ggctaccgcg tctacggtcc
     2401 ctatgacccg atgcacggcc tcagatgcaa ccccaacaaa ctgctgctcg acccgtatgc
     2461 caaggccatc gaaggcaaca tcgacggcga cgaaagcctg ttctcctact ggttcaaaag
     2521 cccggacgac acctcggcga tgaacgacct tgactcggcc gcccacacca tgaagtcggc
     2581 cgtcatcaat ccctacttcg attggggcaa cgaccagcac ccctacatct cgtaccacga
     2641 ttcggtgatc tacgaggcgc acgtgcgcgg catgaccaac ctcaacatgg acgtgccgcc
     2701 ggacatccgc ggcacctacg ccggcctggc ctacccgagc gtcatcgaat acctgaagaa
     2761 gctcggcgtc accgccatcg aactcatgcc gatccaccag ttcgtcaacg actccttcct
     2821 gcaggagaag ggcctgtcga actactgggg ctacaacacc atcggcttct tcgcgccgca
     2881 caacgcctac tcctcctccg gggaacgcgg cgagcaggtc aacgaattca agtccatggt
     2941 caaggcctac catcacgccg gcatggaagt catcctcgac gtggtgtaca accacaccgc
     3001 cgaaggcaac catatgggcc ccaccctgag cttcaagggc atcgacaacg cctcctacta
     3061 ccgactggtg gaaggcgacc agcagcacta cttcgacacc accggtaccg gcaactcgct
     3121 gcttatgcgc tcgccgcacg cgctgcagtt gattaccgat tcgctgcgct actgggttac
     3181 cgaaatgcac gtcgacggct tccgcttcga tctggccgcc acgctggcac gccagttcca
     3241 ggaggtcgac aagctgtccg cgttcttcga catcgtggag caggacccga tcatctcgcg
     3301 cgtgaagctg attgccgaac cgtgggatct gggctccggc ggctatcagg tgggcggctt
     3361 cccgtccagc tggtccgaat ggaatggccg ctaccgcgac acggtgcgcg acttctggcg
     3421 ctcacagccc tccacgttgc ccgaattcgc atcacgcctc atgggcagct ccgatctgta
     3481 tcaggtcaac ggccgtcgac cggtggcgtc cgtgaacttc atcaccgcgc atgacggttt
     3541 cacgatgaac gacctggtca gctacaacga aaagcacaac gaagccaatg gcgagggcaa
     3601 tcgcgacggc gagagcaaca atcgctcttg gaattgcggc gttgaaggcc cgaccaatat
     3661 tccggacgtc aacgacttgc gtcagcgtca gatgcgcaac atgttcgcca cgttgctgtt
     3721 cagccagggt atcccgatga tttgcggtgg cgacgaagtg gcccgcaccc agcagggcaa
     3781 caacaacgcc tactgtcagg acaacgagat ctcctggacc aactggcatc ttgacaaggg
     3841 acgcaaggaa ctgctcgcct tcgtttccaa actcattcat ctgcgtttgg atcatccggt
     3901 gttgcaccgc cgtcgcttct tcacaggccg tgagcccggc gacgattcga acatgattcc
     3961 gcaggtcgaa tggttcgacc acaccggctc gatcatggat atggacgact ggcagaacac
     4021 gcatgcgttc tccatgatga tctatctgaa cggttcggat attccggagg tcgactggta
     4081 cggcaaccgg atggtcgaca atgacttcat cctgatcttc aacgcgcatt acgagccgat
     4141 tatgttcacc ctgccggatg agcgatacgg ccgcaaatgg cagctggtcg tggatacgca
     4201 caacccgaat gagccggcgc tgagctatga ggcgggcttc atgatcaccg cccagtcccg
     4261 cagcttcctc atgctcatga gcgccaaaaa gcccaagaag cccatgggcc tataaccaga
     4321 ccgaatttaa gctccctctg acgagggagc tcccggtgta gccgggtgag ggagagaccg
     4381 cagcaaagcc gcgaacccct actcgcgccc cttacgctcc tccacgagcc gagcatcccc
     4441 gaacgcaagt agatgctcgc cggcaagcgg gcgcggctgg tcgagcagct cgcggaaatc
     4501 caggattcca tcgacttcat cgacgccaaa cagcgcttct acgacgacgt cctgtctggc
     4561 gcaaccccat acgtaagcgt gctcatccca tccgcggcgc tcgcaaagga gtcagaggaa
     4621 ccgggagaat tgaaggggtg gagaggaaat aggttcgacc gagctgtttc ttcctccggg
     4681 atacaagcgc gaacaacggt ttgttgtacc cgcgcttggc gaacgggaca tgcccttaga
     4741 ctggaatcag caagcgcaca gcgagacgcg cgaatcgttt tccgatgtcg ggaggtagtc
     4801 atgtcgatcg gtcaggttat caaagtcgtg cgcgaggagc gaggtcttac gcagtcgcag
     4861 ttcgcgcacg agctgttcgt cacccaacag gcgctttccc gctgggagaa gggaacggca
     4921 gagccgagca tcgacatgat tcggcttatc agcacccgtt ttgaggtgcc gatggcgagg
     4981 cttatggaaa tgccggacaa tggtttttgc caaagttgtg ccatgccgtt ttaccgcccg
     5041 gaagatcacg gcaccgaacc ggatggaacg cgaaacggag attattgcaa ctactgctat
     5101 gaagacggcg nnnnnnnnnn gcgtttttct tcaggcctat gccaatagcg atgaattggt
     5161 cgccgcgtgt gcgccgatga tggcggaatc ctgccatatc agcgtcgaac aggccgagga
     5221 ctgcatgtcg gcgctgttgc caaacctcaa gcggtggcgg cgtcaggatg agatcgatgc
     5281 ggtggcggag gggaaatgag cgatggcatt cgactataaa aaggaatata aggacctgta
     5341 tcagccgaag aggatgcccg ccatcgtcac ggtgccggcg atgcggttcg tcgcggtgga
     5401 cggtgtagga gatccgaacg aagaaggcgg cgactacgcc aaggcgatgc aactgctgta
     5461 cggcatttcg ttcaccatca aaatgaacaa gaagtcaaat aatccggacg agcacattga
     5521 cgggtacttc gactacaccg tgcctccgct ggagggctta tggagcatgg agaagggtgt
     5581 gcccggcgtc gattacacgc gtaaaaccga cttctattgg acttcgatga ttcgtctgcc
     5641 ggaattcgtg accgatgagg tctttgcgtg ggccaaggca tcgtttgcct ccaagcatcc
     5701 cgaagtcgac atcaatcgtg catatctctt cgacttcgat gaaggcgtgg tcgcgcaggt
     5761 catgcacaaa ggcccgtacg acgacgagcc cgcaaccgtg acgattctgg acggctacgc
     5821 gcgttcgcaa ggctacgggc tcgacttctc cgacgcccgc cgccatcacg agatctacct
     5881 atccgacccg cgccgagcca aaccggaaaa tctcaaaacc attatccgtc atccggtagt
     5941 gaaggtgggt tgactttcgg cgaactgctc gaagcgctca ccgcgcagta gcgtgaacaa
     6001 tctgccggaa tccttaccgg ggtcaaagag cgctaccaag aactcgataa gcaaaataac
     6061 tagaagcacg ggttcctctc ggagagattc tgcccgagcg accttataaa tccttgcgcg
     6121 ggtcaccttt ccctgtgcag gaagcatcca ggctcatgtg cattgatgac gccgatggat
     6181 tgcagatagg cgtagacgat ggtggtgccg atgaatcgca tgccgtacgt cttgagatcc
     6241 actgcgatgg cgtcggacag aggggatgta gtcatgcctg tctcgtagat gacgtgacgg
     6301 ccggtgaacc gccaaatata gtcgtcgaaa ctgccgtacg acgtctggat atcccggaat
     6361 gcgttggcat taccgaccgt cgcgacgatt ttgcgacggt tgcggatgat gcccggatcg
     6421 ttcatgagtt cggcgatctt gttctcgtca tagcgccgga tccgttcgat gtcgaaccca
     6481 tcgaacgcct gacggaaacg cttgcgtttg ttcagtatcg tctcccatga cagtccggct
     6541 tggaaggtct cgagcaccag catctcaaag aggtggtggt catcatgcga cggaaccgcc
     6601 cattcggcat cgtgataggc aacatagacg ggattgctga gattgcacca gccgcagcgt
     6661 ggtcttatat cgtccatgtg ctctccctat gggtcgttgg agacggtcgg ttctagatgt
     6721 tgattcgatg tgtgatctag tataaacggt gtgttcggat agcggtctgg atgtgcggat
     6781 tgtctgaacc atatttgccg gattgcaggt ataaaggggt tagttactgc ggttgcgggt
     6841 atttcggtac tagttagggg tgattctggg ctgtaactcg acacctttta cctgcaaccc
     6901 actcccgccc acgggattgc acaatcggtt gcaggtattt tggtgtcatt tgagattggg
     6961 aagcggaaat cgatgtcgca tggaatcgaa ctgactagca ggttggggtc ccaacatcac
     7021 aaaagtccct tataccgcaa ggccatatag gttctacggt catcggataa aaccggataa
     7081 atcgtttagc ggtcggataa aagaagaagc ccagaatcgt tgagattcca ggcttttttg
     7141 tttgtcgggc tagcgggatt tgaacccacg acatcctgct cccaaagcag gcgcgctacc
     7201 aaactgcgct atagcccgat gcgccattaa ggcacaatga atcattgtaa acgacgctgg
     7261 atacattgcg tgtcgagcat gtgaattcaa aaggttctgg attctcttga tagcttatga
     7321 gtgacatctt tattcattga tgctctacaa cgactaggtt tggatctgca tcgatatccg
     7381 gcgcatccgc tggactaaca aggaccgtgc aaacgatgtg gcccccgact tcctgcgagg
     7441 aagtcggggg ccacatcgcc catctgtgcg gcaaggacgc gcaggaacat gtgcttcggc
     7501 ttcggcagct cgtcctggtg ctgagcctcc tcaagcatga agctggagta cggcaacagg
     7561 cttaccgcga acggcgcggc accatcggcg cggctgacgc gcatgccgtg gccgtgatcg
     7621 tcggtaatct cggcccaacg cacatcctca tggttgcccg tctcctgcgg catgaggtac
     7681 ggcgcatgat ccgcgaaagc gttggtgctc cacacgccga gcttggcgtg tttgcggtcc
     7741 aggtacgtct ccgccgggcc ggtgccgaag aacctcaggt tcgtgtactg cacaggcagc
     7801 gtccattcga tgccgaacgc cgggatggtg ggcaggtcac cctgctctcc agggtattcg
     7861 acgtgcaggt tcacgcggcc atcggtgtgg gccgtgtagg agacggtcac cttggtgcgc
     7921 tgcgcggtgg cgagctcata cgtgtacgtg cccttgagcg tgctgtcgtc gatctgctcg
     7981 agcacgttgt ccacgcagcg ggcgtagcgg ccggcgccca gccactggac gcgctcgaaa
     8041 ccgtgaccgg cgccgcgatc gttgtcggtc agcggacgga aggtggtgat tgcgggacgg
     8101 cgcagcacga actcgttgcc ggcgaaggta taggagacca tnnnnnnnnn nggccacggt
     8161 gatggtccca tcggccggct tcgtgtcggg cgtcgcggtg gcgtccgcgg gcaccacggt
     8221 ctgtccgaag gcgagctcgt agccgctttc ggcccaatcg gtcgccttgg cgagacgctg
     8281 cgaaacctgc agcaccagtt cgcgggcgtc ggcgcggtac gccgccaccg gccatgcgac
     8341 atcgaacgtg cgggtctcac cggcgggcac gtcgaaacgc cgggtggact gccagaccgg
     8401 cttgccgtcg gcgagaacgc tcaggacgaa cacgtagtcg ccggtggcgg tgaacaggtt
     8461 gtcgttcttg acggacaccg agtccttcgt cacgtcgatg tggacgttcg agtacatctg
     8521 cttgacttcc tgggccatgg gggaaggctt gcggtcggcg aacagcaggc cgtcgccgga
     8581 gaactcgtag tcggacggac ggtcgccgaa gtctccgccg tagcgcaggc tcctggtgcc
     8641 gtcgggctgg gtggcgtaga tggcctggtc
//
LOCUS       DAWBMW010000059         8556 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_1983_length_8556_cov_3.071756, whole genome shotgun sequence.
ACCESSION   DAWBMW010000059 DAWBMW010000000
VERSION     DAWBMW010000059.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 8556)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8556)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8556
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1983_length_8556_cov_3.071756"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    553..562
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1952..1961
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4088..4097
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1712 a         2727 c         2367 g         1720 t
ORIGIN      
        1 tggttatatg gccgctgatt tcggtaccga aatcagcggc ctttccgttt accgtggcga
       61 tatggatgaa aggcgatcag gcagctacgg tgcccaggga agtcggcgat cataaacgtc
      121 tcgtcatttt catcgcggat cttcagttgt ctaatccacg ggaggcgaca ggcgtgaagt
      181 ttctcaacga agcggaagcc gggcacaagt cgtatgttac gatcgacttc gtgcccagct
      241 cccgttcttc tgctcagaag cggagtgtgg ttcaccatcc agccgacatc atcggcatca
      301 tgccgcagcg ggtcaacgta tcggccagcg catcgtccag tgcttgaatc ggtcatagcg
      361 acgacgccac cgaacgacga tggacagtat ggcgcctatc gccagagctg acacggccag
      421 aaccgaggca agcgacacat cgccacccgt gacgggcagt gacgggcgag ggttgggcgt
      481 ggttgcctcc gccggcgccg gcggcgtctc gggtttctcc ggttcgcacg gtttctccga
      541 ctcgcatggg ggnnnnnnnn nncaacaccc gcacacgctc ccaggcatcg tcataccggc
      601 tggacgccgg ggaaacacgg tcatccccct cgaaccgcca gacgaacacg taccacccag
      661 gccgttcggc ggtcagatac ataggagcgc catgcgcgtc caacgccccg gcgccgattt
      721 tgaacgtgcc gttcatcgcg ggaatctccc aggtggccag caaccggtgg ttgtcgtctt
      781 ccgtgggaac ctctcccccg gatggcttgt acgcctcatc gttggagggg ttgtcgggat
      841 cgccggacca ccatacgctc accgtcgcat acggccgatc cgccgcgaac tcatactctt
      901 cgttgccggc atactggcca tgatccgcag ggaatcccga tacggtaatg gtgtcgctga
      961 gctcggcacc gatatccgcc gaatgctcag tgaccgtcga catgacttct agcttcctgc
     1021 gacttgtatt gctttccgta gcctccataa acggacttat ccagtcgcct ataaggtatt
     1081 cctgcgcctg tttgctctgc tcggaccgcc gaaacaccca tacccatgtg ccaaaaccgc
     1141 tattctgctt ggtccggtaa gcggcaccgt catccggctt ggtcatggcc tgcacacgtg
     1201 cctgctgccc caccccggtg aaagaggcct tgccataggc caccggctca taacccaaag
     1261 tcgccaatcg cgcaagaaaa gcatcggcgc tctcttgtgc attcggcgta atgacattgc
     1321 ccacatcgcc cgtatcaaga ccatcgaaat agtatccgcg ggcctgcaat tccaaatcgg
     1381 gcacccaata actgtccgca tccgccacgc cactggtcac gtcatcgaac accggagaag
     1441 ccacatcaag caccttctcg gagaccttgg tggataccgc cggaacaaaa tccttacgca
     1501 cccgaaatgt cgtcgacgcg ccgccagtgg aagccatcga gtcaaaggcc agcatgtcct
     1561 gagtgctgtc catgacgtgc atccgcccat actcgtatgt ggtgttcgcc gtcacctcgc
     1621 cggcgccggt cgcctcccac gcgatcgagg acccggcgct cgtcgacacc cccgagaacg
     1681 tgttgccgcc ttggacgaac cgcgccgccc cctgcaaggt gacggtgaac ggcactcccg
     1741 cgatcgcgtc accggcgcgg ttcaccactt tcaccgagat ggaaccgctg cgcaaagccc
     1801 cggcatcggt gcgttccacc gtcgtgcccg caggcgtctt gccggccgat tgatcccata
     1861 tccgggccgc cttcgccacg atctcggggt accggccttg aatgaccgcc atctgtctcg
     1921 cccactcgtc ccgatcgcgc ccgaagtgat cnnnnnnnnn naagtgatcc tgcacgatga
     1981 tgccgagcgc cgcatgggtg gcggcatcca tgtcgcggta ccggtcaagg atccatgcca
     2041 tacgccgggc gttctcatcg gaggcaagca acttggtggg gcctatcaca tagtcgctca
     2101 gtttccccgc ttcgatgcag tagtacttgt tgcccgaagc gtccagcccc gcgacaccga
     2161 cgtaatattc catatcgttg tatggaaacg ccacgtgata tctttcgtcg gcgggcgtca
     2221 acgtcgcggc atacgccgta ttcgccgagc cgaacaccac tcccaccatt attatcagac
     2281 cggtcagcat cgctacgatg gccatgcccc gttgccgcca tgtcgttatc atcattcgtc
     2341 ctttcctggt acgtttcccc gcatactgca ctgtgtgcgg gtaggccacg atcatggcag
     2401 attttcattc gctacagccg gtttttcgac ctatgtggtc cgagcttgcc gtttgccggc
     2461 gagttgtgga taactcttcc gccgagttat ccacctcatg cggttgaccg aacgtttacc
     2521 tgatagttat ccacaatgcg caacatatgt tcaaaagccc ttagaatagg gccttatggc
     2581 agcagtacaa catcgcgcga ccacgcgcac tagtaatacc gataacagca ccacgaaaac
     2641 gaagtcaaag gcgacttctg cccgaaagag ccccgcaacc aagcgcaaga gagtcagcgc
     2701 cgaaacggca cgcgccgcag cagcgctgaa aggcctggct gtcgaagccc ccgcaccgtc
     2761 catcgaagca aacgagcccg gccagttcgg ccgcatcaat gtcatggaca tcactcccgc
     2821 cgaggagcgc ggcatcttcc ccgcccgtgt ggaactcggc gaaccgtttg agatgaccgc
     2881 ccaggtgttc atcgaaggcc gtaccaaggt gggtgccacc gccatcgtgc gcaacccgcg
     2941 cggcaaggaa accatgcgcc gggccatgac ctgcgtaaat cccggcctcg atcgttggac
     3001 cgtgatggtc aagtgcggcg agcacagcga tctgaagcct tgggaggatg gctacgcggc
     3061 cgtcaagcgt cagctcggcg aatggaccgt gaccatcgaa ggttgggaag acgcgtacgt
     3121 ctcctggctg cacgacgccc gcatcaaggt gcgtgtgatg gacgacgtgg acaacgcact
     3181 caattccggt gccgagttgc tggcccgttg ggccgaaacc ccggacaccg gcctgaccgc
     3241 acgcgatcgc aagaccttgg agaaggccgc tgagaccatg gccgatcaga cgctgagcgc
     3301 cgaggatcgt ctcgccgccg gcgacaaccc gaccatcgcc gccctgcacg agactcaccc
     3361 gctgcgcgac ggcatctccc ccagccagcc ccagcgcttc aaggtggaac gcccgaagtc
     3421 cagcttcgcg gcttggtacc agttcttccc gcgttccgaa ggcgccacca tcgatccgaa
     3481 caccggcaag atcattcagg gtacgctgaa gacttcgatg gctgccgagg gcttcgacat
     3541 cgtctacctg ccgccggtct tcccgattgg cgtgaccaac cgcaagggcc gcaacaacac
     3601 gctggtcgcc ggccccgatg acccgggctc cccgttcggc atcggctccg agctaggtgg
     3661 ccacgacacc gtggatccac tgctcggcac catggacgat ttcaaggccc tgtgccagcg
     3721 tgcacatgaa ctgggtctgg aaatcgcgct cgatttcgcc ctgcagtgct cgcccgatca
     3781 cccgtgggtc aaggcccacc cgaactggtt ccgccacaag ccggacggct ccatcgcctt
     3841 cgccgagaac ccgccgaaga agtatcagga catctacccg atcgacttca acgccgacat
     3901 gcccggcatc gaaaaagaag tcgaacgcat catgaatctg tggatcgagg cgggcgtcac
     3961 catcttccgc atcgataacc cgcacaccaa gccggtgcgt ttctggcagg acgtgattgc
     4021 cgccgtgacc aagaagcacc cggagatcct gttcctggcc gaggcattca cccgcccggg
     4081 catgatgnnn nnnnnnnccc agtcccactg ctacttcccg tggcgcaaca ccaaggacga
     4141 actggaagag tatctgccgg tcaccaatgg cgacgacggc tactaccagc acaacacctt
     4201 ctggcccacc acgccggata ttctcaccgc ttacgtgcga gacaacggcg tagccggcca
     4261 ctgcgtgcgt gcggtgctgg cagccatggg ctcgccgtcc tggggtatct acaacggtta
     4321 cgaactcatc gagaacaagc agcgccccgg cttcgaagag cagatcgaca acgagaagta
     4381 cgaggtcaag gttcgcgatt ggagcaaggc caagcagtac ggcgtggccg agatgctcac
     4441 cgcactcaac aagattcgtc gcgcccaccc ggccgcgttg agctaccaca acctcaccgt
     4501 gctgcctacg tccgacccga atattctggc tttcgcacgc cacacgccgg ccgaactgac
     4561 cggtaccggc caagcggaca cgctgattgt ggtcgtgaac ctcgatggac acaacgcaca
     4621 ccagtcgatg attcacctgg aactcagcga gctgggtctg cccaccgacc gcccgctcaa
     4681 cgtgcgcgac gagctgaccg gccgcgaatt ccagtggggt tgggacaact acgtgtctct
     4741 ggctccttgg gcggatgtgg cccacatcct gtccgtgcag tgatgcggta ctgcaacggt
     4801 aatgcaacag tgatgccgtt gacgtgcatg ggtttatcgt gcgcgtaaac cgctagaaat
     4861 cctcccctca aggggaggat ttcgtctgtt tgggcatagt gtcggcataa tctcagcacg
     4921 gtttcttgag ttccgcacct gttttgcagg cgtaaacagg gtattgtggt gagacgataa
     4981 cgtaactgtc gattttcaag gagaaatgac atggcagaaa ccttcaacgt cgtggtggaa
     5041 attccgcgcg gctccaagaa caagtacgaa gtcgatcacg agaccggtcg cgtcttcctg
     5101 gaccgtaccc tgttcaccgc aatgggttac ccggatgact acggctacat cgacggcacc
     5161 ctgggcgagg acggcgatcc gctggacgct ctcgtgatga ttccgaactc cgtgttcccg
     5221 ggctgcgtgg tcgagtgccg cgccgtgggc ctgtaccaca tggtcgacga ggccggcggc
     5281 gacgacaagg tgctgtgcgt gcccgccgac gttcgtttcg atgacatcaa ggacatcgac
     5341 gacgtcaacg agtaccacaa ggccgaaatc aagcacttct tcgagcagta caaggctctg
     5401 gagccgggca aggaagtcct gccgggcgac tactggactg gtgctgacgc cgccgaaaag
     5461 gaaatcattg ccgcgcgtca gcgtctcgcc gattccgaga agtgattcga ttctgaatcg
     5521 ttgacaggag gccccttcgg gggccttttg ttgtatttgt ggcaaagccg ctctactctc
     5581 cctcagtcgc agtttcactg cgccagctcc ccaacagagg gagcttgtat tttgtgatac
     5641 agtaatgttt tgtcttttca actaggcgta ggcggcgtgt aggaacgctg gtcgaggccc
     5701 atcgcacggt aatccggcga tgaaccttct gtcacaccat gatttcgata atcattcaaa
     5761 tcctgcttat ttctgtttcc gtagctatgg atgcattcgc cgtatccatt ggcaaaggcc
     5821 tgaccgtatc ccgcgtacga gtacaagacg ccgtgaaaag caccctctgg ttcggcggat
     5881 tccagatgct gttccctatt ctcggctatt tcgcggcatc tacattcagc aaatacgtaa
     5941 cccaattcga ccattggatc atcttcgccc tgttggtgtt catcggcggc aacatggttc
     6001 acgaagcctt cgaagaagat gaggaaaact ccaaggagac cgcccagttc gattggaagc
     6061 atatgcttcc cctggccgtg gcctgcagca tcgatgcgtt cgccgtgggc gtgagcctgg
     6121 ccttcatgtt caccaaagcc catatggcat tcgccatcct cagcatcggt gtggtcaccg
     6181 gactgttctc cgctgcgggc ctgcacattg gccgcgcgtt cggttcccgc tggcagaagc
     6241 cggcgcagat cgccggcggc gtggtgctga ttctactcgg catcaaagtg ctattggagc
     6301 atttgggcgt tatcgcgttc tgatttagtg ccataagccc cttcaccaga aggggctatt
     6361 ttgtttgatt tcaccgttcc aatgcccgac gtaggattgg gatagaaatt cgattatctg
     6421 agaggcgtat catgaccgag atcacccagg agggttggaa gaacaaagct ctgagcatgt
     6481 tgttggccca actgacgtcc tatgtactgt tcatcgcagc cacggtcatt ccctcccctg
     6541 gcaccgtccc catcatccct ttgattatcg ccgctctgac gttggccgca tttgtcgtat
     6601 tctggccgtt ccgcggcagc atactggatc gtatcgtcac tttggtgttt ggcgcgattt
     6661 ctctgatttt cgtcatcgta cctttcccga ccagcgaggt tccgccggat cagaccgctg
     6721 cggatggctc ggtacttcct tggtattcat gggcactggc catgggcctg ctgctggtgg
     6781 tgctggtggt cttctccttc ggacgccaga tggctcgtga gaagcgcgaa catcttatcc
     6841 gcgcactctc tcacgccgtg acttccggtg tggcagctct cgccgtggcc ggctggtgct
     6901 tcctacctga tttgggcgcg atgctcgcca agggcaccgt tgcgggaacc gttgccctga
     6961 tcatcctcat cgttttggga ctggctttgg ccgtggcttc cacgttgtgg gtgcgcgacg
     7021 ccgatccgga cccggatatc cgttatccgt ggatcggtac cggcctgatg ccggtaatgc
     7081 tgatgggcgt gaccatcgcc gcaaccgcac tggtgctcgg ccgtatcatc ggttgatgga
     7141 cgtccaccgt tccatattcc gcaaacttgt aacctatgtg tccactacaa gcgcgtagaa
     7201 taatgaaaac cgtgcgaaag cggcataacg ccgctgacga ctcgtacagc cacaccgagc
     7261 aattgcgtaa cacattgcag gataaggggg attagcgtga cggatcttac gttgatgact
     7321 ttcgccagcg accccgccac cgttttgcca tcactcgccc tgctttctca tcgcgtccgc
     7381 gtactgccga tggatgcagc cagcttggtg aagatgccgg agaacaccat tctgtttctg
     7441 gatgcacgcg atgatctggc gaccgccaaa accttgtgcc gcctcatcca cgcttccggc
     7501 ctgtccacgc caatcgtgct ggtgctgacc gaaggcggct tcaccgtggt caactcccag
     7561 tggggcattg cggatgtggt ggtcgccacc gcttccccgg ccgaagtcga gggccgattg
     7621 cgattggtct ccgagcgcgg caacgctccc gcagccgcag cagccggttc tcccgccgaa
     7681 tccaccacca cggtggaaga tggccagatt cgctccggcg acctcattgt ggacaccaac
     7741 ggctataccg cctccctgca tggacgcccc atcgatctgg catacaagga gttcgaactc
     7801 ctcaagtact tggtccagca ccccggccgc gtgttcacgc gcgcccagtt gctgcaggaa
     7861 gtatggggct acgactacta cggtggcact cgtacggtcg acgtgcatat ccgtcgtctg
     7921 cgcgccaagc tcggcggcga atacgaacac ctcatcggca ccgtgcgcaa cgtgggctac
     7981 cgattcgacc cgccggagac ggataaggaa actgccgccg cacagcaggc cgccaaagca
     8041 tcctctgccg ccatcgacac cgatgacgat actcccgatc tcaacaccgt cgcctgacag
     8101 accagcggcg ctgcaaacta tcaatcatcg atattacgcc gcttaggtcc atacgatgag
     8161 agaaaccaaa tccgcgcgtc tcacgcgcat gcatgacgaa tacgacattc tgcgccaggt
     8221 gattccggca cccgcttgcg cactgcattt ctcgaatccg ctcgaattgc tgatcgccac
     8281 cgtgctcagc gcccagacca ccgacaagcg tgtcaacacg gtcaccccgg aactgttcgc
     8341 cacctacccc accgcccgtg acttggcggc cgcaaacccg gcccaagtcg aagacatcat
     8401 tcatccgctg gggttctatc gttcgaaaac ccagcatctc atcggactgg ccacggcact
     8461 ggacgaacga ttcggcggcg tggtgccgcg cacgatggat gaactgacca gcttgccggg
     8521 ggtcggccgc aaaaccgcga acgtcgtgct cggcaa
//
LOCUS       DAWBMW010000060         8485 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_1997_length_8485_cov_2.879359, whole genome shotgun sequence.
ACCESSION   DAWBMW010000060 DAWBMW010000000
VERSION     DAWBMW010000060.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 8485)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8485)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8485
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1997_length_8485_cov_2.879359"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1105..1114
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2178..2187
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2668..2677
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5784..5793
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1572 a         2610 c         2496 g         1767 t
ORIGIN      
        1 gagcacttcc ttggtaagga agaggtcgtg agtccaattc tcactcgggg ctctctggcg
       61 gggtagctca gctggctaga gcgtacgact cataatcgta aggtcaagag ttcgagcctc
      121 ttcctcgcta caaatcgcgc ccgctaaccg gtgggcgcgc actataccta agaacacaga
      181 ggtgaatgac aatggctaag agcgccgaca tccgtccggg catcacgctg gcctgcaccg
      241 agtgcaagga gcgtaactac attacgacta agaaccgtcg taacacgccc gaccgtcttg
      301 agctcaagaa gttctgcccg cgctgcggca agcagaccgt gcaccgcgag actcgttgat
      361 cgcaagtatc acagctttac aaaacccggc ccattcggcc gggtttttgc gtattgcctc
      421 cggaggcgag tggcagggtg cggaatcagt atccgcatcg cgtgagaaac ggcagaaatc
      481 ccgcgtctga acgaacccct cgcgggcgtt ctgccgtttc gggccgccaa aacggcagaa
      541 atcccgcgcc accttcgttc agacgcgggc gttctgccgt ttcagccgat gattgcctcg
      601 atatcgcacc tgcgccacta agatagtaag ccatgacaag ctttgcagac atcaccacca
      661 tgggcgtggg cggccccatc gcccatttca tcgagccgac cacgcgcgtg ggactcatcg
      721 aagccgtgga ggaagccgat tccaaaggcc tgcccttggt cgtcgtcggt ggcggctcca
      781 acctgcttgt ttccgataag ccttttgacg gcgtggtggt tcgagacgcc cgacgcctca
      841 tcactgtgcc ggacgaggct gcaccggtag agggcgagga tcgcaccgtg catgtcaatg
      901 ccgaggccgg tgccaattgg gatgatttcg tggccttcac cgtagagctc gggctcgagg
      961 gcgtcgaggg actgtccggc attcccggca cagtcggcgc ttccgtggtg cagaacatcg
     1021 gtgcctacgg gcaggaagtc gccacttcgg tcgaatcggt ggaagtctgg gatcgagaca
     1081 ccaaatcaac gcgcgacctc acacnnnnnn nnnncgccga tcttcgattc ggctatcgat
     1141 actccgcact gaagaccagc atgtacgccg gccccggccg gccggccggc cgattcttcc
     1201 cgacgccacg ttacgtggtg ctttccgtca cctttgccct gacccactcg gccgaaggca
     1261 cggtaggtta cggacagctt gccaaagcgc ttggcgtcga agtgggcgac cgtatggcca
     1321 ccgccgacat ccgcaaagcc gtgctcgcag tacgtgccgc caagggcatg ctcgaggacc
     1381 ctactcgcta cgccttgcct gacatggcca ccgccaagcg cgaggccaat atcctgaccg
     1441 atctggagcg tctggcctcc ctcaatgagg ccgccggcat tcccgttggc gatgacggtc
     1501 tgcctgctcc cgactacaat cgccacagct gcggctcgtt ctttatgaat ccgattctga
     1561 ccgccgacca agcggcggcc ctgcccgaag atgcgccgaa gttcgacgcg actctgcctg
     1621 acggcactcc cggcaccaag acgtccgccg cctggctcat cgaccatgcc ggctgccata
     1681 agggatataa ggtcgatgcg gacgcgccgg ccagtctatc cacgcagcac acgctggcct
     1741 tgaccaatcg tgggggagcg agcgcggccg atatcgcagc tttggcgcgt gcggttcagc
     1801 aggcagtcaa gagcgcgttc ggagtggact tggtgcccga accggtctgc gtcggtgtgc
     1861 tgtaatcgcg ctattgattg atggagtggg ttattaatcc catatattgc ggactctgag
     1921 tcttattaat agacaagaca cgcgggctct tctatactcg attgtcggta ttgtgcgcat
     1981 tcggtgcgca aacaacagag aaaaaagaag agcactatgc aactgttccg tacaaaatcc
     2041 gtcgaacaga ccattgccga aaccggtgaa gagggccgca agctcaagcg caacctgact
     2101 tggtgggatc tgtcaatcat gggtgtggcc gtagccgtgg gcgccggcat cttctcggtg
     2161 ggcgcgcagg ccgccgcnnn nnnnnnngat catcagcttc ctgatcgccg gcatcgtgtg
     2221 cggcgcagcc gtgatgtgct acgccgaatt cgcttcgatg attccggtgg ccggctccgc
     2281 ctacaccttc acctacacca cggtcggcga gatcatggcc tggatcatcg gctgggatct
     2341 gatcctggaa atgctgatgg ccggctccgt gatttccaaa tattggggcg tatacctcaa
     2401 cgatttcatg cgcctgatgg gctggaacgc caacaccaac ctgacgttcg gctccttcca
     2461 catcgatatc gccccgatta tcatcgtggc gttctttacc acgctgctcg tattcggcac
     2521 caagatcggc gcccgcgtgg acggtgctat gaccgtgctc aagatcgcca tcgtgttctt
     2581 tgtggtgatc gtcggcttct tctacgtcaa ggcctccaac ttcaccccgt tcattccgcc
     2641 gagccagccg gcctccaccg tggaaggnnn nnnnnnnatg accccatccg tgtacggcgt
     2701 tcctggcatc ctctcgggcg cggcgctcgt gttcttcgcg ttcatcggat tcgacgtggt
     2761 ggccaccgcc tctgaggaga ccaagaaccc gcacaagaac gtgccgctcg gcatcggcgt
     2821 gggcatgctg ctcatcattg tcatgtacat gctcgtggcc gtggtgacca ccggcatggt
     2881 ctcctatgcc gatctcgcca agcaagacag cccctcgctg gccaccgcct tcgaactggt
     2941 gggcgccgac tgggccgcca agatcatcag cttcggcatc gtgctgggcc tggccaccgt
     3001 ggttatggtg ctgttgctcg gcctgacccg tatcgtcttc gccatgagcc gcgatggtct
     3061 gctgccgcgc ggtctgtcca agaccggcaa gcatggcacc ccggccgctc tgcagatcgt
     3121 cgtcggcgcg gtggtggcgc tcgtggccgc cttcttcgac atcggcgtgc tctccgacat
     3181 ggtcaacatc ggcaccctgt ccgccttcac gctggtggcc atctccatcc cgattatgcg
     3241 caagaagcgc cccgacctgc cgcgcgcctt caagatgccc ggcagcccgt ggattccgat
     3301 cctcatcgcc gtggccaact tctggttgat gctcaacctg tccgtgctca cctggattcg
     3361 tttcgtggtg tggctcgtgg tcggcttcgc catctacttc ggctacggct actggcatgc
     3421 gcgcctcggc aagggcgacc tgaccgaaac gctgtccgcc gaaaccgata aggccgttaa
     3481 cggctgagag cgaggagcgt cccaacaggc gcgtaccctt gtatcagtat ttgttaagga
     3541 ggattccatg ccatatgtaa tcgctcagcc ttgcgtggat gtcaaggaca aggcttgcgt
     3601 ggacgagtgc cccgtcgact gcatttacga gggttcccgc tcgctgtaca tcaaccccaa
     3661 cgagtgcgtc gactgcggtg catgcgaacc ggtgtgtccc accgaggcca tcttctatga
     3721 ggatgatctg ccggacgagt gggcttggta caaggatgcc gctgtcaact tcttcgctga
     3781 agtcggcgat ttgggcggcg cttccgccaa tggtccaatc ggcaaagacc cggaacaggt
     3841 cgccgcgctg ccgccgcaga accagtaagc tgatttgcgg ataagcgctg cacacagcgc
     3901 atagttcgga ttgaaccctc ggtctatcac ggccgagggt tcgtcgtatc ttaggaacat
     3961 accgtccaag aaaggggttc tcatgggctt ccacgcatat tcgtcgccat acgactggtc
     4021 tcgtatcgcc ggcttcaaag ccaccgccaa aaccgtgccg ggtggcatga tcgacctgtc
     4081 cgtaggctcg cctgtggacc cggtgccgag cagtgtccag caggcgctgg ttgccggcgc
     4141 agacgccccc aacgcccatg gatacccggt caccgccggc accgccgacc ttaaggccgc
     4201 tatcgataca tggttccgcg aactgcgcgg ggtggatctg aaatccatca acgctgccgc
     4261 cgtacccacc gtgggttcca aggaggccgt ggcgctgatg gccagcctgc tgcaccttgg
     4321 cgaaggcgat gtggtcgtcc aaccggctgt ctcctacccg acttacgaaa tcggtaccca
     4381 acttgccggc gccaccgtgg tcaaagtcga tgatgtgacc gacgtcgatt cctgggtgaa
     4441 tattccgaac gtcaaggcca tctggatcaa ctcgccgtgt aatccctccg gcgaggtcat
     4501 ctccgctgac tggctgaccg acatcgtggc tgctgcccgt cgcatcggtg ccgtggtgct
     4561 ctccgacgaa tgctatgcgt taatggactg gcgttcggtg cgccgcaata cagccgcgtc
     4621 ctatgcgcct gccgcgtctg catctgctgc gtctgtgcct gccgcaccgg tggccgaatc
     4681 caacgagccc gcctccgata ccgcattcag tctctccgcc acgccatgtg ccctgaatcc
     4741 acatgtgtgt gaaggttcgg cagctggcat cctggtgctc tactcgctgt ccaagcagtc
     4801 caacatggcc ggctaccgta cggctctaat tgccggcgat taccggctgg tcaaggaaat
     4861 ggccgaatac cgcaagcaga tcggccagat tatccctggc ccagtgcagg ccgccatggc
     4921 cgcaggcctg aaggacacag ccgctgtgca cgagcaatgg aggcgttacc gtcgccgcct
     4981 atccgcgctg gttgacgcac tgaaggcata tggctattcg gcacaaatgc cctccggcgc
     5041 gctgtacgta tgggtcaagg ccaagtccgg cgactgctgg gccgatatgg ccgagctcgc
     5101 caaaatcggc atcatcccca gccccggcga attctacggc gctcccgcct acctgcgttt
     5161 ctccgccacc gccaccgacg aggccatccg ctccgcctgc gaacgtctca cggcgtaatg
     5221 agatcgtaag tggcgttgag ccgttcacgc cccaaactgg gcggccttgt tgccgaattt
     5281 gcggcgcaca gcgtccagtg cggcctcggc atcatgcaaa cgcgcctgtg aggtctggcc
     5341 gcgggcattg tcggcctctt cgagcatgtc atccagtgaa ggctgcacgg ccgtagccgc
     5401 cgccttactg agcgaagacg tgctcatacc ggccaagcga atagttttgg gcaggggagt
     5461 ggcagactcc gccgaggccg gtagccccag catgactttc agctgccgca cgcattccgg
     5521 gtacagcacg ccggccgtat cggtcggccg ctccatcgta aaagaccggg tctgatagct
     5581 caggtcggcg aaccgcaact tcaccgtcag cgtgcgcgcc accagcccct tgcgccgcag
     5641 cgtgctggtt acttcgtcgc aggccgaacg cagtagccca cacaccacgc gcatatcgcg
     5701 tgtatcctcg gccaaggttt cctcggaccc aatggatttc tcctcgcgta ccgtggtaat
     5761 ttcgcgggaa tccaacccgt gcgnnnnnnn nnnatgcacg ccccaagcat taagccgctt
     5821 ctccaaagac ggcccgattc ccggaatacc acgcaatggc atcatctgca cgaattcggc
     5881 cgagcgggcc gcgggaatca gcagcatgcc gtccggcttg gcattggtcg aggccatttt
     5941 ggacaccagt ttgttacagg cgatacccac tgaacacgtc acgtggaagc gctcggccac
     6001 ctgagcgcga atccacgcgc cgatcgcgct cggccgcccc cactccagca gtgcggcgga
     6061 cacatccata tagccctcgt ccaccgaaac ctgctcgaac cggtcggtga cggtcaaaaa
     6121 cacctcatgc atgattcggt gcgacatcat gcggtaatac gccatatcca ctggcagaaa
     6181 aatgccgttc gggcacaacc gatgggcgcg cgcggccggc atcgccgaat tgatgccgta
     6241 tttccgggct tcgtagctcg cggcggacac caccgcgcgc ggcccggtgc cgatgattac
     6301 cggcttgcct ttgagcccgg ggttgcgcgc cacctccaat gaggcataga aggcatccat
     6361 gtcgatatgc agcacggtgc atccggtctc gtcgtggccc caatcgcgtt tggcggcctc
     6421 aagtctcggt gcggtgctca tgccaccatt gtaggaatgc cgaacgaggt catgctcact
     6481 atgtggcgcg catgacgcgg cgctcattgt cataatgccg aatatcgccg gaattccaag
     6541 ggtttacgca ctgtgcggct gtttcgcatg gtgggtgcga ttccacggaa tcgtccacat
     6601 tgcgtcactg tccgtaacgc cttccaagtc aatgcgcgct gtcccggctc tccgagtagc
     6661 gtaaacctca tgaagaaggc agtggtcggt atcctcaacc gtgttcccgt ggttcttatc
     6721 gtgattggtg aggcactggt gatttatttg gctacggccg tcgccaaact tgcgttcacc
     6781 cagctcgatc cgctgtactc ggtgtggtat cgcgtgggat tcatggcggt gctgttgctg
     6841 ctgtggcgca ggccctggaa caaggcgaag cgagcgcaac tgtttcaccg cgacgcccgc
     6901 agctgggggc tggtcgtact gctcggctgc tcgctggtgc tgatgaatac cctgttttat
     6961 gtggccatca gcaatatgga tatgggcatt gccgtgtcta tcgaattttt gggaccgctt
     7021 tcggtggcgg tgattaccgg caaaagctgg cgcgagcggg tcggtatcgg tatcgcggcg
     7081 gtcggtgtgg tgctgttagc tggcatatca ttggccaatc cgtccggcaa tggcacgttt
     7141 ttgtatggtc tgattgcgat tttgatcggc gggtcgatgt ggggcatgta tatcatcacc
     7201 ggccggcgcg tggcggctgg cggcaattcg ctggataatc tgtgcatcgc cgtgaccatc
     7261 ggctggctgg tccagtccct gttccttggc gtgccggctg tggctcatgt ggttcacccc
     7321 aaggccgatg ccacgtgggc gcttgagccc ggcggctcgc tgaagctgct ggctctgctg
     7381 tttgtgattt ccctgatggc ctcgttcatt ccctatgtgg tggagcagat cacgctgcga
     7441 cgcaccacat ccggcgcatt ttcggtaatg caatccatca atcccgccat ggcggtggcc
     7501 gtgggactgg cgttcggcga gattcccagc gttggtgaaa tcgtcggtgt ggcgttggtg
     7561 atttgcgcgg ttatcgttac gttctccggc gacgccgcac ccgcggacgt tcaataatgt
     7621 gtcgttcggc acgccgtatc cgcaggacgt ggtacagtaa tttgctgttg cgcattattg
     7681 cgtaacgtgg agtcatgcct gagtggccga taggggcacc ctgctaaggt gttagtcgtg
     7741 taaacggctc gagggttcga atccctctga ctccgccgat taaggttccg ggtgaaagct
     7801 cggaactttt ttgttttccg gaaactccgg gttaagtaac tcctgaaata accgaggacg
     7861 ggcggtctcc gcttgtggta agcggagacg gaggtacgcc attgcccgtc cgcctccaaa
     7921 ccgccatttt agtgggcgaa cgagtgcggc acgtcgggaa aagtccgagc gacgaaagaa
     7981 aaatgtccac taagtgagac ttttgcgtgg tgttcgtgaa tggtgattga agattaaccg
     8041 gtttatatat tgcttgtcca atcgcttgct catcgcctcc ctccccgcag tacttgaccg
     8101 agatgggcgg taccctagag agagaagaag agttccgaag ggcgggcaaa gatgagccat
     8161 atcgctgggc aggggcaaca tcgctccaac tcgacacgaa ccaaccgtgc ggcgctgatt
     8221 atcgtgttcg tgatcgtgct cgccgttgcg ctgggcatcg gcgcatggtt cgcaacccat
     8281 cgcacggacg gcaatggccc cgcacacgcc ggagtgtcgg acgtcgcgtt gaataaagcg
     8341 aagaacacgc ccaaaccggt gaccgaacat cacggcaatt caccggattg cccggatacg
     8401 gactgcatcg ccatgctggt caacggcgat ttgttgttcc atgagggatt gtggagccat
     8461 ttccaaggcc agaaccccaa cgcca
//
LOCUS       DAWBMW010000061         8475 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_2000_length_8475_cov_2.806295, whole genome shotgun sequence.
ACCESSION   DAWBMW010000061 DAWBMW010000000
VERSION     DAWBMW010000061.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 8475)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8475)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8475
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2000_length_8475_cov_2.806295"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1660 a         2514 c         2522 g         1779 t
ORIGIN      
        1 ctggagagca tggcggcgtc caattcgccg accatgtcgt ccggcatgac gtttcccacc
       61 acgtcttccg cccgcagcgt cataacggtg gatggacggc attgctcggc caccacgtat
      121 ttgccatcgc catcctgtac cagcagcagc agatcggcaa agctcaaatc ggcgatcacc
      181 tgccaatcgg cgacaagatg atgcagccac tcgcggtcct cgtcgtcgaa gtcggggcgc
      241 gttgcaagga tatgtgaaaa atcagccata tccctatcat acgcagacgc accgccgccg
      301 tcgcggcaag ccacacgggc aagtacgacg actatgacga catcgcatct tcaataccgc
      361 gccaatcgcg ttgcacggct tcccgcccgt ggccaaggtg ccggcatgtt gcttatcgta
      421 cacggcaagc atcacgtaca gtttgatatc cgaagccgcc accatattgc cggcattgct
      481 gtgtccatgg aacccatcat cgcgtttgcc tgcgctttgg cgttgtcatc gccattgccc
      541 tgggccgcaa gatagaccgg cagatagaaa tcggcagcca cgaattgctt gccggactgc
      601 gacgtggtcg cgatggtggc gtcctctgag gagtcggcgg atgttccatc gatgaccatg
      661 gcggagacgg ccacatcgga ggacgagtat gaatccgcga tgtcagacaa ctggctggat
      721 gtgatcaccg gagtggaagg cttcgacttc tccgtcgagc ttttcttgga cgaatcgtcg
      781 ctgttcttgt ctttggcgct atcagacgtg ttcttcttgg ccttggactt ggcgtcagaa
      841 gaatccgtcg tgccatcatc ggcggaatcc gacgcatcgg ccccggtcga ctgtgccgat
      901 gtttgcgcct gcggctgagc tccccacaat cccatgctat gggtgatgaa caagccgatg
      961 actaacgcca cgacacaaat cagcgcgatg agcacaatca ccaccgcttt ggtgtgatcg
     1021 acgccaggac gcccggcggg aggttcagga gaagcagggg ccgccggatt ggcctcgggc
     1081 tgctccaatg ggcttccgca ctgcgaacag aaggcatttc cttgctcgtt ggcaaaccca
     1141 cactccgtgc aatacaccat cgccgagccc tttattgctg cgacgttctc atcttcggat
     1201 tccatagtat cgccgccatt gggtgatagt gttcgtgcga gtcggtattg acaagcataa
     1261 aagttacgct aacgtaggtt acggcagcgt aagaagcgta tcgtcaactc gtgaaacggg
     1321 ggcatcacca tggcatcaac aaccggcgac tcggaaaaga cgatgcatca cgacgctcat
     1381 aacagcgcga cgattgcaga ggatgccacc atcgcatcca agcgtcaggc ggctatcgag
     1441 gcgcgcgaac aggcgttgca ggccaaggct gatgccgtac gggccaaggc ccagttcaag
     1501 gccgaggcca ttcgagccaa agccgaggag aaggcccggc acaagctcgc caaagcggag
     1561 aaccgtgcac tgaaaatcga gggcattgcc cctgcggaag tggaacgcaa gattcgtctc
     1621 gacgtgcacg gacggccaaa gccgttgatg cgcggctgga ttcacgcgat tgccgctccc
     1681 ctgtcattgg cggcaggcat cgtgcttata tgcttggcac atggcgcgag tctgaagtgg
     1741 gcgtgtgccg tgttcatgac agcgtcgctg attctgttca tcaattcggc ctgctaccat
     1801 ctgggcgatt ggagcccgcg cgtcaccgat gtgctgcggc gtatcgatca tgtcaacatc
     1861 ttcctgctca ttgctggcac gtataccccg gtttcgtttg cgctggaacc attctggcgc
     1921 aatgtgatca tcatatcgat gtgggcgtgc acggccatcg ccatcgtgat tcatgtgatc
     1981 tggatcaatg caccacgctg gctgtatacg gtggtgtaca tcatcttcgg catctacggc
     2041 ctggcttata tggtgatgtt ctggaattcg ccgtatgcgg ggccagccgt ggtggtgctg
     2101 ctgtgcgccg gcggtgcctg ctacatcctc ggcgccatcg tctacgcctt gcgcaagccc
     2161 gacccttggc ctcgcgtctt cggattccat gagattttcc actgcggaac ggtggccgga
     2221 tatgcctgcc atatggtggc catctacatg gtgatcgtct cgctgtggca ataagcgcta
     2281 cgacatataa gtatgccccc gcctacgggg gcataccagt cacttaactc aggccagcgg
     2341 cttggcggcc ttgacagtca cggacagctc gcggccgttc ggtgcggtgt aggaaacgac
     2401 gtcgcccacc ttggcacctt cgatggcctt gccaatcggg gaatccgggg tcatgatgtc
     2461 gtagtcggtg ccggccacga tgtcacggct gccgagcacg taggtcatct cgttgccgtt
     2521 gagctcgatg gtcaccacag agccctcgcc cacggtgcct tccggagcat tctcgatgat
     2581 cttggaatcg cgcagcttga cggtgagctc ggtgatgcgg ccctcgttct tgctctgcgc
     2641 ctcacgggcg gcctggtagc cgccgttctc ggacaagtcg ccctcggcac gcgccacggc
     2701 gatcttgtgc gcgatttcct cacgcagctc accttcacga tgggcgagct cttccttcat
     2761 cttgttgtat gcttcctgcg tcagcaggta gaccttgtcc tcagccatat cgtcctcctg
     2821 aaccgttgtc gatgctgaaa caattttcgt tatttatgac aaaacgccgg ctatgacccc
     2881 ggcgttcgtc atgtgctgac ctatggtcag cgggtggaaa tgatgtcgat gacgaacacc
     2941 agcgtgtcct cgccgccgat gccggcctgc gggatgccgc gggaaccgta gccgtattcc
     3001 ggcggaatgg aaaccaccag acgcgagccc acgttatggc ccggcacggt ctggtcccag
     3061 cccttgatga cctgtcccac gccgatgccg aaactggcgg gctgatgacg gtcgaagctg
     3121 gagtcgaatg gggtgtcctt accccacacc acgccgtggt agttcacggt gacggtgtcg
     3181 ccgcgacgca cgatcgggcc gtcgccctcg gtcagctcca caaccttgag gcccttcggg
     3241 gcttcgggcg tcgggaactc gataacgggc gtggtgccaa attcggcgtt cacctgaggc
     3301 atctgatctg tcattagtcc tccttcaaag acacaatgcg ccctacctta gcaccatcgg
     3361 cgaatcggcg gtagtccttc atccctgtgc gatacgacac gctggccgta tacgtcaatt
     3421 ccggtcatca tgctggtgaa taccggacat gtcaatcagc attcgacgac attgacggca
     3481 agtccaccct tggaggtctc cttgtatttg gccatcatgt cttcaccggt ctgcttcatg
     3541 gtttcgatgg cctgatcgag ggtcacgatg tgtgagccgt cgccgagcat ggccatacgc
     3601 acggcgttga tggcggtgtt ggcggccatg gcattgcgct cgatgcatgg aatctgcacc
     3661 aatccgccca ccggatcgca agtcagaccc aagttgtgtt cgatgccgat ctcagcagca
     3721 ttctccacct gttccggcgt gccgcctacg actgcggcaa gtccggccgc cgccatcgaa
     3781 caggccgagc cgacttcgcc ctggcaaccg acttccgcac cggaaattga ggcgttgcgt
     3841 ttgaacaggt atccgatggc accggcggtc agcagaaacg tgaccacgcc ctgttcattg
     3901 gcgttgtcaa cgaaatgcca gtaatagtga agcacggcgg gaatgatgcc ggcggaaccg
     3961 ttggtcggag cggttacgat gcgcccgcct ccggcattct cctcagagac ggccaaggcg
     4021 aagagatcca cccatgcggc gtcggaagac tcgagcacgg cgtctttgcg ccggctgtcg
     4081 cggcgcagca catcggaatt cgaggcgaga cgccgataca ttttcggcgc gcggcggggt
     4141 acgtcaaggc caccgggcaa tgtgggttcc gccgaggtgc atccgtggtc cacacaggcg
     4201 cgcataacac gccagatggc atcaatatcg gatcggacct ggatgcccga gcgtgaagcg
     4261 gtttcgttgg cccatacaag ttcggcgatg ctgagatgat gttcccggca taacgacacc
     4321 agttcagtgc atgatgagaa cgggtagggt gcctcgacgc cgaaatcagt ggatgatgac
     4381 tcgtccgcat cggcaaagga ggcgccttcc ggagggcgtt catgaatgcc gatcatcggg
     4441 tcttcgggag cgcctttgcg cacgaatccg ccgccgatgg aataccagac ctgttcgtcg
     4501 accaagcttg cggcggcgtc gaacgcctgg aatcgcatgc cattggggtg cgcggccatg
     4561 cgcttccatt gttcgaacac cacgtcacgg tcgtagtcga aatggatgcg tttgaggccg
     4621 gcgaggttca gagtatcgtc caacgcgcag atctcgcgga tgtgcatcat gtggtcggta
     4681 tccacggtgg ccggcagatt gccttcgagc ccggccatcg tagcacgatc ggtgccgtgg
     4741 ccgagaccgg tcagcgccag agaaccataa agcgtgattt tgacacgtgc ggtgcgatcg
     4801 accaaatggt cggcctgcaa cgaggaggcg aaagcatgtg ccgcgaccat cgggcctacg
     4861 gtgtgcgagg atgaaggtcc gacgccgatg gtgaacatgt caagcacact gaacatttgg
     4921 ctccttctcc cctgctggtt cggcatgccg gtgttaccag gcgtcatgcc atcacgatat
     4981 cgtatcccag cccagcgccc ggaatcgctt tcgccttgtg cgatgatggc cgcgcgcgtc
     5041 gtgccggcga gcgataagtc agtcagtgag acagtgcgga gaacatcatg accagtacga
     5101 tgataccgat gatggccaat acgatctgca ccaccatcag cccgatggtc agtcccttct
     5161 tggtggcgat tttgggcgcg tacgcctgtg ccaacaggaa ggcgatgaag ccgaacaccg
     5221 cgtagttgac ggtcagggcc atcacatcgc cgttcgacaa caccacgccg ttcagcggct
     5281 ggttcagcgt gttcatcatc gcgctcatca cgaacatgcc ccacacgccg tagccgatta
     5341 atccgacgac gccgtacagc catggcttga gtttgtcgtt ggcacgcagg gtcagggctt
     5401 cggcacacca caacagggcg aagccgacga tgtaggtgaa cgccacggcg atcagtatca
     5461 cgatagtcgc ccatggcagg agtgcggcca cattgccgat catggacaga tagaatcccg
     5521 agcaggcgat ggcaccaacg gtgagtgcag tgcccgcaat gacggcttgg gcaagcatgg
     5581 tgggcagggc gccttgttcg actcgttcga catcggtcca gcgtgaagcc atgtggattc
     5641 ctcgtttcat caggtacatc gtcacaacgc cacatagcct aacggccttt tgtttcggcc
     5701 ttatgagctc tccacatagt gagctgcgag ccccctctga cgaggggggc tgtcggcgaa
     5761 gccgactggg gagagaccac ggcacagccg caaaacggca tcggccacag gccgatttca
     5821 aggtgcctcc ggtgggactc gaacccacaa gccgaagcga tcgattttaa gtcgactgcg
     5881 tataccattt cgccacggag gccaactttt ctattataag ctatctcctg ccctgagcat
     5941 tgcttgcggg ttgctgcact tcgtgcagcc gtcgcggcat agccgctccc ctcgcctaca
     6001 aacagcccac tgggctgttt gcttaacggc tcagcccgct ggaggcactt accacttacg
     6061 ctattgagcc agcaaccggc gaccataatc gagcacaatg ccgtccagca gaccgtgttc
     6121 gctggcaacg aacgaatcga tggcttcacc atgatcggcc ttggcggctt cgctgacacg
     6181 cgccagcaca cggctccaga ccacagcgcc gccgccgacc acatcgatac ggccggggtg
     6241 gatggtcttg tattcgcggc gctcggcgcg cgtcatccta aggaacttgt cgtccacggc
     6301 ataggcgtct tcaaggctca accgatgccc gtcgaccacg gtgtggtcgt attccttcaa
     6361 tcccatggcc aaggcggtca tcgtggtcac cgtgccggac acgccaatga tggtacgggc
     6421 cttgcctgcg tccacggtgc ggaaggcctc atcgatatgt tcatcgacgt ccacaacggc
     6481 ctcatcgatc tgtgtctggg tcggcggatc attggtcaga tgacgttcgg tcatgcgcac
     6541 ggaaccgatg ttcatggaaa acgcgccctg aacctgggta gtgggcgcac tgacgccgtc
     6601 gccaccgatg accagctcgg tggacccgcc gccgagatca accaccagat agggggcggg
     6661 cagatcgtcg cgattgacca cggaagtggc accaaggaag ctcaggtcgg cttcttcggt
     6721 gccgggaatg acttcggggc gcacgccgag gatacgttcg atctcatcct cgaactcctc
     6781 acggttttcc gcgtcacgag ttgcagaggt ggcaacgaaa cgcaggccgt cgatgggatg
     6841 ttcggcaatc acaccggcga attcccgagc ggccacatag gcgcgttcca acgcttcgtc
     6901 ggcaaaacga tgggtcttgt ccacatcctg gccgagacgg atcacacgca ggatgcgcgg
     6961 caccacctca tgcatgccat cggcatcgac acatgcgatt ttcagtcgaa tggagttcgt
     7021 gccgcaatca atgccggcga cggttacgga ttccttgctc attgccttct cctcgtatcc
     7081 cttagtgcgt atgaatggac tgttcgcgct ggctcatctg ttgatcaatc gtccagcgtg
     7141 cagcggcaga cggtcggatc gaattcgtct ttgacgcgtt ccagtaccaa atcgccaatg
     7201 gggttgacgc ccggtcccat aaccagggat ggggccagca gggcatgcag gcacttgacg
     7261 cgcaccggca tgccgccggc cgaagtgcct tcaatatgct cctcactgtc gccaagacgt
     7321 cctgccagtt cgtggcggaa tgccagatac aggttatggg cctgctcata tgcggccttg
     7381 agctcctcgt ccgaggccaa catgtcgttg tattgctgca tcacgccggc ggcctcgaca
     7441 tgggaggcgg ccttgacggc ttccggaccg gtcagatagc acgtggtggg gaaagggatt
     7501 ccgccgggca acaccggacg ggtgattacg gcaagtggcc taccgcagac gcatcgcgcc
     7561 cccacggcga ccatgccgcg cgggtatcgg ccgagctgac gctgcaccag gtcgatgtca
     7621 tgatcgttgg cgggctcggc aagcacggta tcgacaaggg ccttggcctt ggcggcgata
     7681 tcgatggtca ctgttgggtt ccctcccctg tgttctgttg ggtgttctgt tgggtgttct
     7741 ggtctgactt atcggaccgc ttggatgagt cgttggatga cgagtcagag gaaccggaaa
     7801 tatcggattt gccggactcg ctatcggtca caggctcatc ggcctttttg agcgagtaag
     7861 acagttcgct gtaccatggc aggacttttt tgttgctgct actggaatct tccgttttct
     7921 gagattcgct atcggagccg gtcacggctt cgggatgaag cacacgcacg gcctgttcgc
     7981 cggggaatac gaagccgagg cgttcacggg cctgagcggt gatgtaggcc ttgtcatccc
     8041 aacgcttgat gtcgttttcc aactcctgct ttttggcgat cagcgaagcc tcctcacgct
     8101 tgagaccatt gagttcggcc aggttcaggg cataggtgtg gaaggtggag accagctgga
     8161 tggcgccaag ggcgataatg aacagggaga cgaagaacgc tataggcccc gccgaccctt
     8221 ttttcctaac ttgtgctttg ccggttttgc caggtttgcc ggtacgggac gacttgctca
     8281 tgtcacacga aaatacccgc cgcattcgac ttgacgaata cgacgggtat cgagcttcag
     8341 tggaaactca cgcctttacg ggcgtttcag ctcacttggc caggtacttc ttgcaggcct
     8401 tgaaggcgct gtagccggcg tactgagcgg tggagccgag ctcctcctcg atctcgagca
     8461 ggcggttgta cttgg
//
LOCUS       DAWBMW010000062         8408 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_2013_length_8408_cov_2.567341, whole genome shotgun sequence.
ACCESSION   DAWBMW010000062 DAWBMW010000000
VERSION     DAWBMW010000062.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 8408)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8408)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8408
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2013_length_8408_cov_2.567341"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2274..2283
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3562..3571
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7939..7948
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1686 a         2541 c         2623 g         1528 t
ORIGIN      
        1 atcgtcgtga cttccgccgt gacgatcgcc gcgacaacaa ccgtcgtgac ggcgaattcc
       61 gcgagttttc gcaggaagag aagcgccagt accgtgagga gaagcgctct gaatacatga
      121 ggcgcccgcg ccgtaactcc gacggcacga tgagcttccc ctcgcagaac ccgtacaccg
      181 accgtcgtcc cgacgagccc aagatgccca agggcatgga atggtcgatg ctgtccaagg
      241 atgagcgcga acgtctgcgc ggcctgtcca aggagcacgc ggagaacatt ggtctgcaca
      301 tcctcgccgc tttcgcgctg gaggagagcg accccgaact tgccttggag cacgccaagt
      361 gggtggcccg tcaggcctcc cgcatcgact tcgcccgcga gacgcttgcg ttcgtggcct
      421 accgtcaggg cgactacaag ctggccctgc gtgagttccg caccgcctac cgtatgaacg
      481 gcttcctcga ctacctgccg ttcatcgctg actgcgagcg tggcgtcgga gagccgaaga
      541 aggccgtgga actggcgctg tccgatgagg gcaagcagct gcatggcgct cccaaggtcg
      601 agatgttcct ggtgtacgcc ggtgcccttg gcgatttgga gatgtgggac aaggccattg
      661 aagtggttca caccgtcggc cgttcccagg ggctgccggg tgagtaccgc atgcgtgccg
      721 tgcaggccga gcagtacttc cttgaagagg ccggccgcag caaggaagcc gagcagctca
      781 acgacctgct ggaccgtctt gaggaccagt atgccgacga ggagattgac gaaaacagcg
      841 acgacgtggt gatcgatcac gatctcgaag acatcaccga cgactccgac gtgctcgaaa
      901 agctcggcat cgatccggat gaggcgcagt acgccccgga tccggagcag gaatacggcg
      961 atgaggatga cgaggctgaa gacggtgatg ccgagtctga tgaggccgac gagaccgaga
     1021 ccgattccgc caatgacgcc gaggctgacg attccgccga atccgccgac tccgactcca
     1081 ccgaacccga aaccgaaacc gaggagtgaa cggcgtaatg acccgtttcc tcaaaggcac
     1141 cgatcgcccg ctcgccgagg cctatcagct tgcgctgctg gacctcgacg gcgtggtgta
     1201 tcgcggcaag aatccggtcg aatacgcggc cgattccatt cgtgcggccg aagccgccgg
     1261 catgaccatc gaatacacca cgaacaactc ctcgcgcttc cagcacgtgg tggccgacca
     1321 gctcaagggc ttcggcttgg acgtggagcc gtggcaggtc atcacctctt cggtggtggc
     1381 cgcccgtatg gtggccaagg cgttgcccgc cggcgcgcgt gtacaggtat tgggcgccga
     1441 gcatttgcgc gatgaggtca cccgcaacgg cctgaccatc gtggacggtc ctcaggaccg
     1501 cccgcaggcc gtcattcagg gctggtatcc ggatatgact tggcagatga tggccgatgc
     1561 cgcgttcgcg gtcgaggccg gagccacgta tttcgtgacc aaccgcgacc taacgattcc
     1621 tcgtgaactg ggcatcgccc ccggttgcgg atcgatgatt cgcgccgtga tcacggccac
     1681 cggcgtcgaa ccggtcgcct ccgctggcaa gccggaagcg tacatgtacg atgaggcccg
     1741 cgaactgaac gccgccgaag gccacgattt ggtgcccaag gaggcgtcca tcgccattgg
     1801 cgaccgcctc gacaccgata tcgaggccgg taaccgcggc gactacgatt cgctggcggt
     1861 gctgaccggt gtcaccaacc cgaccgaact gatgctggct ccgtcacacc tgaggcccac
     1921 attcatcgcc cctgatctgc gtgaactcgg cgaagcccaa ccggagccgg tccgcgatga
     1981 gagcggtact tgggaatgcc gcaaggctag cgcttggttt gaaaacggcc aggtacacgt
     2041 cagcgacccc accagtatgg acggcctgcg cgccgccgta tgcgccgcat gggaagccgc
     2101 cgaccaaggc gcacagctta gcgaggccac ggtaccggtg ttcgccatcg aggcatagtc
     2161 ctatgcctac ggatagcgaa catgcgccgg tcaccgagcg ataccccgaa cttgcggata
     2221 tcgatgaaca agacaacgac cgcaagcttg agacctatcg caacgtgctg gaannnnnnn
     2281 nnngtgctgg aatccttaca acacgagctt gataacagcc gcagctagca gaggtgattc
     2341 attctcccgc cagcggaaga tgacgtgaag tgctggatgg tggctccatg ccgagccacc
     2401 ccgttggtgg atggtgggcc gaaaggaaga tatatgaccg agcagcatat cgatcccgcg
     2461 actccacttg accgacttga catgatgctc gtggcttcgg gactggtcga atcgcgggcc
     2521 aaagcgcagc gactcatcaa ggccgggcat gtgcgcgtgg acggcgagac catcaccaag
     2581 ccatcgttca tggtcaaagc cgggcacagc gaactcgccg tggataaggg cgacgactac
     2641 gtctcccgag gcgcttacaa actcttgggc gcgttcgaaa cctttgccgg caatggcctt
     2701 accgggccgg aaggcttgga atgtctggat atcggtgcct ccaccggtgg cttcaccgac
     2761 gtactgttgc gcgggggagc ggccaaagtc gtagcgctcg acgttgggca cggccagctc
     2821 gacccgcgca tcgccaatga cgagcacgtc atcgaaatga gcggcgtcaa cattcgcgaa
     2881 gtagaggccg acgacctgcc ttaccggccg gcgatgatcg tttccgacgt ctcctttatt
     2941 tccctgacct atgtgatccc ggtaatcgcg cgcatcgcgg ctcccggtgc gcagattgtg
     3001 ttactggtca aaccgcagtt cgaggtcggc cgggcaggac tcggcaaaaa cggcatcgtt
     3061 gaagatccgg cattgcggga gcgggcatta cacgacgtgg tcgcctgtgc tgaacagcac
     3121 ggacttgacg tggtggccac tgccgatagt cccatcatcg gcacccacgg taatgaggaa
     3181 tacctgcttt acgctgtctt gagatgaatg cgttggcagt ctccgttcac cagcagcacg
     3241 ctggaggtac ggtcgatgcc tagtgcggtg gcacacgtgg cgctttccac cgcactgccg
     3301 ttgcgtagca tggcggagtc cgccgtcaaa gtccggcatt gcagatggtt ggcttcaacc
     3361 gtgacggctg tgatatggcg aaccgtgctg ccatcctcat acccatccat atcaaaacgg
     3421 gaaaacatcg tactgcggag tggatgaact tccagagccc tgctgttgac gtcatgaccc
     3481 ttcaacacgc cgttcaagcg gactgtctga ccgctaatat gacgggtgcc tagggagcca
     3541 ttgacggaca gatttccgga annnnnnnnn nacgatatcg ccgttgcatc gtagtttgcc
     3601 ggtaaaggtt atttcatcgc aaataatgtc gccgccgact tcgatgtcgc cgctgccgct
     3661 gatgctggat acgcgaatat caccatcaca ccgcacaatg ccacttttga tgaccagttt
     3721 gtccccttct ataactgagg cctcgagggt gctggtcaca atcatggtgt gaaaatgcgc
     3781 gccatcggca agacgggaac gttggtccac tcctatggtg ccgtagactc ctttgcctat
     3841 cagcgtgcaa ttctgcgtat gcttgcccat gatgataaaa ctccccttat taatatggac
     3901 gtacagcttt atggcttatt gccacaataa tgagcgcatc catgttgagg gggttatatc
     3961 ggtcacgatg tgagcagata gggggtatag cgagcgcagt tagggggtta gccttggatg
     4021 aagtggtcga tctgatcctg cttgcccatg aggatgagtt cgtcgttgcg atgcatgacc
     4081 agttccttgg agccgtactg gaactccttg cctggggcct tgaggcccac cacggtcacg
     4141 ccgtacttgt catgtacttg tacgtcctcg atgctgcggc ctaccgcgta caggggcgtg
     4201 tggaccttga cgacggtgta cggcccgtcg atttcgatgt aatccagata attgccggcc
     4261 accagatggc ccacgcgctt gccggcatcg gtctccgcgt tgatgatgtg gcgggcgccg
     4321 atgcgctgca gaatgcgcgc atgctcctgg gacacggact tggcccacag gtcggaaacg
     4381 ccggcgtcca gcaggttgcc ggtgatgatc accgaagcct cgatgctgtc gccgatggcc
     4441 acgacggccg tgtcgaactg ggaggcgtcg atctgctcga tggccagcac atcggtcata
     4501 tcggcctgaa tggtgggcag atgcgcggac caacgggcca caagctccgg gttcttgtcc
     4561 actgcgagca catcctgacc catctggtcg agggtggcgg ccacggaccc gccgaaacgg
     4621 cccagtccga tgaccagcac gcttttgttg ccgctcggtt ttgctgccat gatgatggtc
     4681 ctctctatgt cagattgctg ggcgccgatt gaatggccga tcagccaacg ataatgtttt
     4741 cctgcggata acgaataggt tcgggagcca tcggtcggct gatggagtag gcgatggtca
     4801 acgggccaag tcgtccgaca atcatctcgg cggacagaat aaacaggctg gccggattgc
     4861 cggcgcttgc cacgcccacc gaataaccac ccaacgagaa ggccgagcag gcttcgaaca
     4921 cggcgtccac gaacgcgcag ccggtggtga acatcagcat catcgcaccg accataacca
     4981 acgcaaagca ggccgtggtc acggacacgg cggtcatctg aatacggcgg gggatacggc
     5041 ggcggaacac ggtgacatcg cgatggccgg tgaatgcggc gcggcaaatc aagagaatca
     5101 ccgcgaacgt ggtgacacgg atgccgccag cggtggatga ggaacctgcg ccgatgaaca
     5161 tgagtatgct catgaacacc ttggtctcat tcgtgacttc gggcacccag gaaatatcga
     5221 aaccggcgga gcggggcatg acggcggcgc tcatcgcacg gcgcatcttc atgccggggt
     5281 catctgcggg gaacaggccg gtattgctcc actcgaccag caggaaccaa gccagtgagg
     5341 tggccaccag cacagcggtg gtcaccaaag tgagtttggt gtgcaatgtc caacgtttcg
     5401 ggctgagccg ccggcgtgcg cactgcacca gattcagcac cacggggaag ccgagcgttc
     5461 cgatgaatgc actgatgaga atcggcagcc ccacgcccca acggttcaca tgcagaccag
     5521 tggcatccgg cgtgaaaccg gtgttgttat aggccgacac cgcgtagaac agcgcctgcc
     5581 acaaggtacg ccccatattg ccatggttca cgcggaacag gtcgggcagc agcagcacaa
     5641 aggtcagcac ctcggcgatg atggaggtgc ccagcaccac ggtgagcacg cctttgacct
     5701 cggaaagctt cgtggtgccc agctcgttcg cggtgagcat acgctgggag accttgagtc
     5761 tgcgggaagc gccaagagcc accatcgagg cgaaggtcat gacgcccaga ccgccgaact
     5821 ggatggagaa caggacgacg acctggccga acatcgacca gtattcgccg gtgttcacca
     5881 ccggaatgcc gcaggtggac agggccgaga cggcggtgaa gaacgcgacc gggaaattgg
     5941 tgaagatgat gtccgatgtg gagatcggca gcagcagcaa accggtgacg atgatgatca
     6001 gtgcgccgaa atacaccatg gacagacggc ccgggtgcct gagcagacgg tcggtgatgc
     6061 tgatacgtcg aatgcgcttg gcggttttat cgcttttgtc tcggctctgt acatcggctg
     6121 aataccacca tgacaggccc ttcgaggcct cctcattggc ggaggattcg tcgaagtcat
     6181 tggccatggg tcctcaagct cctcaactga tactgctgta cggtcatacg gcatgccatc
     6241 gcgctgcgca cggcacgcgt acggtgattg cgcggctgta cgccattgta tgccgaagcc
     6301 ttacgataat cgactctgtg gaacgttgcc ggcgacgcgc cacgaagcag catttttcga
     6361 acacctgtac gactgatgac ggtgtctgcg gttagaataa tgagcagtgc gaccgaatcg
     6421 acaggaggat gccatggcca agcgcaacgc ggtggtggtg acgcatacca ggctcaggca
     6481 gaccggaacc gtagtgggcg aggcggtgag ccagctgcga gtggccggtt tcgaagtcac
     6541 tatcatcgac aacaccgagg cccccgattt cggcgtgcag ccgccgtgcg tatcggacga
     6601 caccgaaatc gtcgtggtcc tgggcggcga cggcacgata ctgcgcgccg ccgaactcgt
     6661 ccactgcacc caggtgccga ttctcggcgt gaacatgggg catgtcggct tcctggccga
     6721 attcgaaagc ttccagatcg acgaggcgat ccgccggatc gccacccacg actattcgat
     6781 cgacgaacgc atgatcgccc atgtggacgt ctggctgccc ggcgccacca agcccattga
     6841 ggattgggcg ctgaacgaca tcacactgga acgcgccgac cggggcaaga tggtcgaact
     6901 gtccatccgc gtggacgatg tggagatgaa ctcgttcggc gcggacggcg tcatcgtctc
     6961 cacgcccacc ggttccaccg cctacgcctt ctccgcgggc ggtccagtca tgtggccgaa
     7021 cgtcaaggcc ctgcagttga ttccgttggc cgcgcacgcc ctgttcgcca ggccgctgat
     7081 catcggctcc ggctcgacct tcaccatcga tattctggac gattccatgt ccgaaggctg
     7141 gatttgctgc gatggacgcc gtcagcgcgc cctgccgcag ggtacgcgcg tcatggtgcg
     7201 cgaatcgcgc gataccttgc gcctcgcccg actctccggc gtgccgttca ccaaccgtct
     7261 ggtctccaag ttcgatctgc ccgtggtcgg ttggcgcgag cacgcgcgca acgaggcctc
     7321 gagccagtcg ctgcatcacg ggcacacgtt cccggcggcc gcgtatgcgg ttggcgttgc
     7381 cgttgccggt gacgctggcg tcgctggtac cggtcccgac aagtcgggcg aatgtgacgg
     7441 caaggcagga tcctgatgct cgaagaactc gatatccgca atctgggacc gattcgagaa
     7501 gccaccattg cgccggccgc aggcatgacc gccatcaccg gtgagaccgg cgccggcaaa
     7561 tccatgttgc tctccgcgat ccgactggtt tccggcgggg ccgccgaatc ctcgcgcgtc
     7621 tcgtccgggg cggacgaggc ttgggcccag gcgattttcg cgctctcgga tgatgccgtc
     7681 gcatctgaac attccggcga caccaacgat gccggttctg gcgatgccga cagtgggttc
     7741 accggtgctg ccgcggccgt ggccaaggcc catgacgcgg gcgtcgaccc cgaagacggc
     7801 gaactgttcc tttcccgcac cgtacgcgct tccggacgtt cgcgggccgt gctcggcggc
     7861 aagtccgtac cgcgttcggt gctgggggcc atcgccggcg aactggttac cattcatggc
     7921 cagaccgatc agctgaaann nnnnnnnngc cgccatggat gagcggctgg aacgcctgcg
     7981 ctcgcaggaa tcctctgccc gccaacaggc cgactacctg cgcgaatcca tcgaccgcat
     8041 caaccgaacc gacccgcagc cgggggagga tgaggagctg aaggcgcgcc gcgaacgcat
     8101 cgaaaacgcg gccgacatcg cccaaggcgt gggttcggcg ctgggcgcgc tggatgcccc
     8161 gcaggtggat gtggacgccg ccgacggcgc ttcggccgtg gaactcatca atcatgcggt
     8221 gacctcgttg cgcgcgattc gcgtcggcgg cacgttcgca gagcaggccg atcggcttga
     8281 atccatcaat gccgatttgg gcgacgtggt gttcgccctg agccaggagc tgggcgacga
     8341 aggcagcgtg gaggatctgg accagatcaa cggccgtatc cacgagcttg gcgacctcac
     8401 ccgtcggt
//
LOCUS       DAWBMW010000063         8159 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_2076_length_8159_cov_2.580084, whole genome shotgun sequence.
ACCESSION   DAWBMW010000063 DAWBMW010000000
VERSION     DAWBMW010000063.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 8159)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8159)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8159
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2076_length_8159_cov_2.580084"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    7627..7636
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1626 a         2464 c         2329 g         1730 t
ORIGIN      
        1 ccgatgaggc acacgatcag atgatcgagt tctgccacgt cgtcaagaac gtcccgccgc
       61 tgctgctcgc cgagcagctg atgctcgact acaccgaccc cattctggaa acgaatgtga
      121 tgggcgtcga tttcaccaac ccgttcggcc tgtccgccgg cctcgacaag aactgcgaca
      181 tgcccgtggt gctggaccat gccggcttcg gttttgaaac cgtaggctcc accacctcgc
      241 gtccctgccc gggcaacgcc aagccgtggt tccaccgtct gcctgaatac gattcgatga
      301 tggtgcacgt cggcctcgcc aacatcggtt cggacaaggt catcgagcgc gccgagaagg
      361 cttggactca ggcccgccag atgcagctgt ctgtgtcgat cgcccgcacc aacgacgacc
      421 agtgcggcga tttggacgaa ggcattgagg attactgcat ttcgatgcgc cgcgccgccg
      481 gccgcaccgc gatggtggag gtcaacgtct cctgcccgaa cacgcacgtc ggcgaaccgt
      541 tcaccgcgac tccggaagcg ctcgaccgtc tgtttacggc gctcgacaag atcgaccgcc
      601 cgcagcccac gctggtcaag atgccgttga acaagccgtg gggcgagtac aaggagctgc
      661 tcgatgtgct ggccgatcac aatgtgcagg ggctgtccat cgccaatctg cagaaggacc
      721 gcaccggcct cgagattccg cgcgattggg agggcggcct gtccggcggc ccgtgcacca
      781 acgccagcac cgagctgatt cgcaaggtgt ataaagagta tggcgaccgg ttcgctatcg
      841 ccggcatcgg tggcgtcttt acccccgagc aagcctatgc gaagattcgt tccggctcca
      901 gcttggtcat gttcatcagt tcgctgatgt accgaggccc gcagcagatt accgtgttga
      961 agcgcggtct ggcccagctg ctgcgccgcg acggattcga gcacgtctcc gacgccgtcg
     1021 gcgtagacgt ggagtgaact cacatattgc cctattaccc atggggcggt aaaaccctca
     1081 tggggcggtg attgtcccat ggggcggtta gccaaatttc acattccgca gaataccgcc
     1141 gttttgggtt accgccccat gagggtttta ccgccccatg ggaaagttac caccccatga
     1201 ggatttagta cgcaccggcc acgtattgcg gggcgtaggg gatctcggtg gacggtatgc
     1261 ccagcttgtt ggctgcacgc aacggccagt acggatcgcg caatgcggcg cggccaatct
     1321 ccacggcatc ggcttcgcca tgggccagaa tcttggccgc ctgcttcggc ttggtgataa
     1381 gacccaccgc ggtcaccgga atatcggcct tggccttgac ctgctcggag aacggcacct
     1441 gatagttcgg cttgaccgga atcgacactc ccgacatgat gccgccggta gacacgtcca
     1501 tcacatcgac gccatgctcc ttcagcacct tggatagtgc gatggtctgg tccaaatccc
     1561 aaccgccggc ggcccaatcc gtggccgaaa tgcgcaccag aaccggcacg ccctcaggga
     1621 cggccccacg cacggcatcc acaacctcga ccaggaaccg agagcgaccg gtcaaatcgc
     1681 caccatattc gtcatcacgc tcattgctca gcgggtcaag gaactgagaa atcagatagc
     1741 catgcgcggc atgaatctga atcgcctgga agccggcggc caccgcacgg cccgccgccg
     1801 cggcgaacgc gccgaccaga ccatgaatct catccacgga cagcgcgcgc ggcgtacgca
     1861 acccaccgaa cgcgatttca gacggtgcca tggtaggcca gccgcccgcc tcctccggca
     1921 cgctctgacc gtcgtaacca acggcaaagc aaccagtcga agccttacgc cccgcatggt
     1981 tgagctggat ggcaggcacc gcacccgcct cgcgaatacc gtccacgatc cagcgccaag
     2041 catcaatctg cccgtcttcc cacaggccca gatcgcacgg cgtgatacgc ccctcaggag
     2101 ccacggcggt ggattcgaca atcaccatgc cgaatccgcc gaacgcgcga gacacgtaat
     2161 gctggtagtg gaacggcgtg ggcctaccat cgcgggcgaa cgacgaatac gtgcacatcg
     2221 gcggcaacca aatacgatta cgcacggtca cctcgcgcag cgtcattggc gcaaacagcc
     2281 ccggacggtc cttgctgact ttcttatctg ccttgctcac agcttccctt tcctcataat
     2341 cctcataatc atcgaacgat tcgctcgtct tgcccgcgcc ggcccgcttc ttggaccgtg
     2401 catcggattt tgacttcttc ttaccgtgct tacggttctt cgatgcatcc tcgtgcttgg
     2461 tcttcggctg cttggactga tcgccgtccg aagacgaagc cagcgtcacc gctttgagat
     2521 ccggcacgtc gaatccacga aaatcaaaat tctcatgcgt cagatcgtcg ttgctcatcg
     2581 ttcccctttc cgatgcacgt tcatggcaaa tattcgatga acgcgatggc gttgtcatta
     2641 agtattgtaa aacgggcgtc acctgaaaca ggtaacgccc gcaaactacg taatttgggt
     2701 atcgcagtcg gccatgttac gcagactcag ccggcatgca atcaccaaat gtcaaacgcc
     2761 aatcaccaat cactggccat cattcgactg ctctgaattg gtgccctggg tttgggtgtt
     2821 gttttgccca gtgttctgcg actgagactg attgttgctg ctctgcgatt ggctgttgcc
     2881 attcgtagtg gtctgcggca cgctgaacgt tcgtccgcca atgcccagat acttgccagt
     2941 cgtggtgtac ttcgagacgg gctggccgta atcgttgtca atcggtattt tcttcttgga
     3001 aatgtagcta ttgaagaagt tggtaacggc cggagccgcg attgtcgaac catcccaata
     3061 gctgttgtac cggccgttga tggcaatgtt ggaaatacgg ttggtgtacg ggttctgtgc
     3121 atcgccgacc acagcgaacg ccgcaatctg attaggaatg aacgatccac cggacaccgc
     3181 aagatcctca ctggtaccgg tcttaccaaa cgacttacgc ccgtcagcca atctgaatga
     3241 ccaaccggca ccatccggcc gcaccgtacc ttggttcagg gtataagcga gtgtttggat
     3301 gatgtcctta tccaccgcct gatggcagtt cgcggatgga accttgagat cgttgccgtc
     3361 cttatcggtc accttgctga tggcaatcgg gttgcattgc acaccattcg aggcgtatac
     3421 ggcgaagatg ctcgccatgg tcagcgggga cacattcacc gagccgatca tcatggatgg
     3481 tgtatacacc tgggttttgt cgatggtctc acccgtggcg gcgtcatggt atcccagttc
     3541 agtcgcggtg tccgccacct tgcacagttt gaggatggca cccatggaag cctgagtagt
     3601 gttatgcgaa cggaccagac cgaggaatgg gctttctggg ttcactgtac ctgcgcccat
     3661 ggcgttcgac acgttccacg aatcggcacc gcccgaatag ttactgcagg cgaagtccgt
     3721 agtggggtat gacgtcgagg tctggaggtt gtcgttgatg gaatggcccg cttccatcca
     3781 cgcaatcagg ttgatcggct tccaggacga accgatggtc caaccagaac caccgccatc
     3841 cgccaaatcg acggcatagt tctggctcgt cttggtggga tcgttcgcgg ctgccggagt
     3901 ggcgtcatag taacgattca ggccaaagct cagcacttcg ccggttcccg gtttgactgc
     3961 ggccatggct atctccatgc cgctgggatc gtccggtgga atggtattac gcgcggtctc
     4021 catcatcgtg gaattcgctt ccacatccag cgtggtgacg attttcaggc caccctcctg
     4081 cagcagcttg ttacgctcgg cacgagtctt gccgaattct tcggagttct ggatgcgatg
     4141 caccacaaaa tcgcagaagt acgcgtaatc gccggtgtcc tgacagccga cattcacgtc
     4201 ctgtgctttc agcgtgtcct tcaacggcgt gttcacagct tcggtgtact gcttctgggt
     4261 gatgtatcct tcctgcagca tcagcttgag cacgatattg cgttggtttt cgctttcctt
     4321 ctggttctct tcgaccagcg gatcatacag gcttgggttc ttggtgatag ccgcgatggt
     4381 ggcggactgc accacgttca aatcggcggc ggacacgctg aagtagcgct gggccgcggt
     4441 ttccacgcca tacaggttgt tgccgaactg ggcgatgttc aaatagccct gcagaatctc
     4501 ggccttggaa tacttctttt ccatctgcac ggaaatcagc atctcgcgaa tcttacgggc
     4561 gatggtgtct tcagtggcat ggtactgggc gatcgaatcg tcatcctcga tcgcctgcat
     4621 cagcagcacg ttcttgacgt actgctgggt cagcgacgaa ccaccctgct gggagccttt
     4681 gacgaggtag gtctgcacga acgcacgcat cacgccttgc acgtcgacgc cggcgtgagt
     4741 ccagaatcga cggtcttcgc gcgccaccac tgcctgctgc atggttttgg aaatcttctt
     4801 cagcggcacg acgatacggt tttggttgta gaacgtcgcg ataacggtgg aaccgtctcg
     4861 cgcgtacatc gtcgacttct ggggcagact agtcacgtcg aagtccacgt tttctacctt
     4921 gagcgacgga atcaccgctt tggcggcttt attggcaccg atcacacccg gtacaaacaa
     4981 cacgctggcc gccacgccgc cgcagacggc aagcatgata taggccaaga ggagaacaag
     5041 cacacgttgg agagtgaggg acttcttttt gggcatgggc cccattctaa atggggtgcc
     5101 ctaccaaccg ggcttacggt cagctcccgt gaacatatca tcacaagaat cgcactagcg
     5161 taacattggc tggaagtcag ccgaacggtc agcgccgcga gcgtcgaatc agcataccgg
     5221 gctggaagat gatgatcgat cggccgtcac gtgcgatcca tccgcggttg gaaaagtcca
     5281 tcaacgcctt gttcacggtc tcacgactgg agcccaccag ttgggccagc tcttcctgag
     5341 tcaggtcatg cggcactttg aggccctgtt ccaccggctc gccgaaacgt gagcccaagt
     5401 tgagcaatgt cttggccaga cgcgccggca catccatgaa taccaagtcg gagatgcgtt
     5461 cgttgttgcc gcgctggcgg ctggccatga cctgcaacat gtcgatggcc acacgcggat
     5521 gctcatcaag ccatgcgaac agggcatcat gctccagcca gaccacgcgc gtgccatggc
     5581 tcatggacac cgcggacgcg gtgcgcgggc caccatgagg gtcgaatacc ggaatctcgc
     5641 caagcacttc gcccggcgcg tggatggaca gcagctgcac gcggtcatcg gaagaagtgc
     5701 gaatcagctt gacgcgcccc tgctccagta aatacatgcg ctgatcggta tcgccttcgg
     5761 agaagatata gtcgccttta tcgaacgtcg catgcttcaa atgcggcaac agctcctcgg
     5821 cttcggctgg cgatacctgc ttgaacaatg ccgtatgcaa caggatgttt tcctcgtcag
     5881 caaaagcgtt tggcgtttcc aagctcatcg caccatcctt attgtcctat gaccgtcgtc
     5941 gcccatccgt catattgtaa cgcgccccac aggtttcgtc aggagcatgc cctcattcgt
     6001 cacttcatgt gtggcacgat ccgatagccg tacgacatgc tgacgaatgc gttaccgcga
     6061 aaaacggcac tgtcgcggct accgcgctcg cccatgacaa aaccgcgcac ggccaccggc
     6121 aaaatcgtat acggccatcg gtgaaatcac gccgcaaacc agtcaataag gtgctcgacg
     6181 atgtgttcgc ggctgagacc cgtctggctt ttcatcgggt ccacgcgttt gaccgctgat
     6241 ttcaccgctt tgtctgacat cttctcgcga ctggtgttga gcacacggct catcttggcc
     6301 gcatcgatgt catacgccat ggtcacatgg tgcagcaccg cgccgcctga gcctacgggg
     6361 aagcgccgct gcgccgctcc cccgatcttg ccgtactgcg aggcgatatc attgaggccc
     6421 gcgaaccgca catccaagcc aagttcgcgc agcgcttcga ccagccacca gtcacagagc
     6481 cggtaggatt cttcgatgct tacgccctga acgaaatcga gcggcgcata cagagaatag
     6541 gtaatggtgt tgcccggttc gataaacatc gctccgccac ccgtgcaacg gcgtaccacg
     6601 tcgaagccga gctgcttggc aatgtccaga ttcacctcgt cctgggccga ttggaaacgg
     6661 ccgatgacca cagccgggcc cgcccactcc cataatcgga tggtcggctg tcgtgttcct
     6721 gctgcgactt cacgcgccca ggtctcgtca atggccattt gctcgtccgg agtacgcgga
     6781 tggtcgtgga tcacggtgag ttgaggcttg agggcgttcc agcgctcccg gtattcagag
     6841 cttttgtcgg gctgcttggt ttgtgtattg atggatgctg ggatgttcgg cgcatcgggg
     6901 ctgcccaccc ccacccgctg cgctggagcc cccgccagcg ggggtaagtt gctgcttacg
     6961 gctcggctgt atgcggtttt gatggcaact tcatctgtgc cgataatttg gcaactagga
     7021 tgagcgtcca atacggaccg caatgaatca tcgctaatta gagctcgttc caggtcatgc
     7081 aacaaagcat gggattcggc atccgaaact gattcaataa agaaatcacc gctgatatgg
     7141 catgactgtg cggtgccgtc atcatcgacg gatacggtca cggcgactag tttgctgcca
     7201 acggttttgc attccccatg tcgcatttgc atgcaactca gtatagagag ttttctggct
     7261 cccctcagcg aggggagcca ggtttgaggt cacatgcggg ccagaatgtc cttgccgacc
     7321 tggtcggtgg agcgcacggt ggagccgagc tcctcgatgt ccgcttcgac ggcggtatgg
     7381 atcttcttgg ccgcatcatc aaagccgaga tgctcgagca gcatggcggc ggagaggatg
     7441 gcggcggtcg ggttggcctt gttctgaccg gcgatgtccg gagcggaacc gtgaatcggc
     7501 tcgaacatgg acgggaactc gtcggaggcg ttgatgcagc cggaggcgga gtagcccacg
     7561 ccgcccacca cggagccggc ctcatcggtc agaatgtcgc caaagaggtt gtcggtgaga
     7621 atcacannnn nnnnnntcgg agaccaggaa gatggtggcc gcatcaatgt gctggtagtc
     7681 gtgggtgacc tcagggtatt cctcgccgac cttgtcgacg atgcgctgcc acatatcacc
     7741 ggcgttgacg agcacgttct tcttgtgcac cagggtgacg tgcttcttgc gcttcatagc
     7801 gagcttgaag gcgtaacgca ccacacgctc cacaccgtat gcggtattga tggacacctc
     7861 ggtggccacc tcctgcgggg tgttgcggcg caccgcgccg ccggcaccgc agtacagacc
     7921 ttcggtgcct tcacgtacca ccacgaagtc gatgtcgccg gggttggcga gcggcgaggt
     7981 gacgcccttg tacagcttgg aggggcgcag gttcacgtac tggtcgaggt cgaagcggag
     8041 cttgagcagc aggccgcgtt ccagaatgcc ggccttgatg cgcggatcgc ccacggcacc
     8101 gagcagaatc gcgtcgttgg ccttgatgcg ctcctcttcg tcctcgggca gaatcgcac
//
LOCUS       DAWBMW010000064         8007 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_2119_length_8007_cov_2.650025, whole genome shotgun sequence.
ACCESSION   DAWBMW010000064 DAWBMW010000000
VERSION     DAWBMW010000064.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 8007)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8007)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8007
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2119_length_8007_cov_2.650025"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2406..2415
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7781..7790
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1623 a         2251 c         2466 g         1647 t
ORIGIN      
        1 aaggcggccg tcactcgccg ttcttctcca actaccgtcc gcagttctac ttccgcacca
       61 ccgacgtcac cggcgtcatc gagctgccgg aaggcgtcga gatggttcag ccgggcgacc
      121 acgctacctt caccgttgag ctgattcagc ccatcgctat ggaggaaggc ctgaccttcg
      181 ctgtgcgtga aggtggccac accgtcggct ccggtcgtgt gaccaagatc ctcgcctgat
      241 tttcatcaga caagaatctt cgcttgaact agcgttatag gaaatcccct tcgaggaaac
      301 tcggagggga ttttctataa ccgcagcagg atatggatat gtaccacgac ttccggcctg
      361 gatgcgctag tgttgctttt caatagataa acggactgct gcactgcgag gatatgacac
      421 tgatggcagg ccgggaaaga aggtcgatga tgactggtgc acaggccgct cactgtggtt
      481 ctgtatccgc aatttcgctg ggactgcccg tttccacggc gattcccgaa gccaaaggca
      541 cactgccgaa agccttgttc gtaggcaaag cgccgatttc cggcaaactc aagcagcgat
      601 tggtgaacga gatcgaatcc atcaccatgc tggcgttggc ccggtcggcc aacacggggc
      661 ttgcggacgg caaactgatc ccggaagtgc tggtcatcgg actgaagctt acggccaaag
      721 ccaccggcat accgaacgaa gtcatcgacc tgatcgccgg acagcgcaaa tcgggcatcg
      781 tgttcgtgtg cgtacgcgac gtgccgttcg agggcagcac gcgtgaggaa tgcgcgttcg
      841 ccgtgcgccg cgccataccg gggcgtgccg ggcacacgcc gatctatcag gtatatgccg
      901 gcgactggca gccggcgggg gaggcccagc tggagctggc cggctctact attgatgagc
      961 tgtgggcatc gctgtgctca cagaccattt tgggtacgcc ggaggtcgag aatctggatg
     1021 cgcgcatcat tcgccatact gaaattgctc ggctcgaatc cgaagtggac aagctcactc
     1081 gtgaccatca gcgcgtcaag aacccggccc agcgcaacga gatctatgcc aaactccaca
     1141 aggccaaggc gcagctcgca aaactgcgcg aggcatagtg atgtgaaagc cattgggctt
     1201 ccgctggcgg gagctggctc gcgaagcgag actgagggtg gtccaggtcc gtggcctagg
     1261 gctacgccca agctaattga aatattcgtg catcatacag cgtacagtta aaccatgttc
     1321 cctctgctcg aaaccctggt cgccgtatac gaggtgggtc agttcacgct ggccgccgat
     1381 gagctgaagg tatcgcagtc gacggtgtcg tcgcgtatcg cccaattgga gcagatggtc
     1441 ggcgcgccgc tgttcgtgcg caatgcgaaa agtgacgtga cgcccacaca ggccgggcgg
     1501 atgctgtacc agactgccat cggcatcgac ggattatggc gggatacgcg tgagcagatc
     1561 gcccgcgtgc aggaggcgcg tgagccgctg gccgtgtcgt tctcgcacac gaccgcggcg
     1621 attctgctgc cgaaggcgtt gccggtgatg gctcgggaca tggacggttt cgacttcgct
     1681 gtacacgtga tgaactcgga tgcgattctc gaacaggcca gcatgaaggc cgtgcagctc
     1741 ggcgtggtgg aaaagccgat cgtgaacgat tcggtggacc gcgtcaccct gtgcgaggac
     1801 cggctggtgt tggcggggga gcctgacggc gtgtggatgg tgcgcgaaca cggttccggc
     1861 gtgcggtatt acacggatct gtatttcaag accgctcagg ttgtgcctgc acgcacgatg
     1921 gaagtgtcca gtaatgcggc gattgtggcg gcgctgtccg ccgggttcgg gcagtcgttg
     1981 atttcggaag ccgccgtgcc ggcgggcgtg ccggtgcagc gggagcttgg cgatgagttt
     2041 gtgcggcggt tctatgcgct ggtgccgaga tccggtctga cgcacgatca gcgggagttg
     2101 gcggagacga ttattgctgc gctgcgcggg tagtcagata gtcgactgag gggaggttgc
     2161 gaacgttgtt tctctccctc cgtccgcctg cggcggccac ctctctcatc agagggagat
     2221 caattaggta tcgacaaaat cgatgatatg tattggttgc aatgatttga ccgatgtaaa
     2281 cgcttgagct tcaatagaca tcatgagaga gttctgtaca aaatggtgga agcgcattgc
     2341 caccgttgac atgctgttca ttggtgttct gactctgctg gcctcgctgt tcgcctcctg
     2401 gctcannnnn nnnnnttccc cggattcagc ctgttcggcg cgctcatcat cgcgctgctc
     2461 atcggtatga tcatccagtt cccgatccgc agtgcctacg tcggttccaa tgatggctgc
     2521 aaggcaggcg tcaaggacgc cgctggcctg atttccaaca agctgctgcg cctcggcatc
     2581 atcctgctcg gtttcaagct caacctcgcc gtgctgttca cgcagggcat caagtgtctg
     2641 ccgatcgccg ctgtggtcgt tacgctgacc atcatcgtgt gctatgccat cgcccgcaaa
     2701 ctcggtgtcg acccgatgct cgccatcctg accgccggtg gcaccggcat ctgcggtgcg
     2761 gccgccgtca tgggcctggc cggttccatc aaggtgcctg aggacaagca ggacgagaag
     2821 gacaacgacg tgaccatggc cgtggccatc gtagccatta tgggtactgt gttcgcgttg
     2881 ctggagatcg cgctcggccc gttgaccggc atgaccaagg accagctggg catcaccgcc
     2941 ggtgcttccc tgcacgaaat cgcccacgcg gtcgcgggcg gcgacgcgtt cggcgcggtg
     3001 gacatcgcca ccatcatgaa gctttcccgc gtgctcatgc ttgtgttcgc ggccatcatc
     3061 atcgccatct ggtgggagaa gaagcattcc gaggttcaga gcaccggcaa gaagaccgtg
     3121 gccttcccgt ggttcatgct cggcttcatc ggcgcttcga ttatcggcac cttcgtaccg
     3181 ttcgtcacct ccatcacccc gcagctcgtg gactttgcgt acatcgtgct cggcatggcc
     3241 atggccgcgc tcggcatcaa cgtgaacttc aaggccatcg cttccaaggg caagaagccg
     3301 atgctcgcca gcttcctgac atcgattctg ctcatgtgct tcgctgcagg tgttgcgatg
     3361 ctgttcttct gataagcgct ccaaatacaa ataggcgaag gcgaacgaca gtgagcaatt
     3421 ggaatgtgtc acctaaaaat cacgtacttg cacaacggat aaccccagcc gaactggcag
     3481 gccatcgcaa tgaacttcac cacatatgga ctcagcccgg tcgcacccgg catccagccg
     3541 atcagcagcg tgctgaacgt gaagttccag atccacagca gcacgaacag cgccacggaa
     3601 cgggtgaagt tgctggacga tttgaatgtc acgttgcggt tcatcacgaa gttgtaggtc
     3661 gccgaacaca ccacggcgat gccgttggcg atctgtgagg gtacgccgat caagtgcaga
     3721 atggcgaacg tcgcatactc cacaatggtc tgcgtggcgg aaacacccac atacttgccc
     3781 agcacggcga gtccattttg cttcggggtt gccgtgccca cggtattttc cgcagtattc
     3841 gttgtggtcg aggtatttgt ggcgcccaca ccttgattcg gcttcacatc cgctccattg
     3901 ttcgccatgc ccatatacct cccggatgta cctgtgacgt tcgacctcgc aatgataccg
     3961 ctgaccgcgt cgggaatcgg cgtggcggtc agacgagaaa accgcaggcc gcagcaacga
     4021 ccggcacacc cagcgacagc accagatagg ccgctgcggt ggggtagcgg tgtttgcgga
     4081 agagcgtcac catttcgttg attgcggtcg agaacgtgga gaagccgccg aggaaaccgg
     4141 tggccagcac tagccgccat ggttcatcaa gtgtcccgcc gagtgccagc gccgcgacca
     4201 aaccggctag gaatccggcg atgagattga tggtgaacgt ggaaagtggg aatgcgcgct
     4261 gccagtacgt tttgatggtc acgtcaagca gatagcggca ggaggcgcct acgccgccgc
     4321 acaggcagac caagattggt aggaatacgg tcatcacgcc ttacctccct tgctggtcgt
     4381 agcggcattc gtatcagcgg acgcttcggc ggatacgttc gaggagccgg ccagatgcgt
     4441 tcctgcccac acgccggcag acgcacaaac cagacccagc gcgaacgtca ccagtagata
     4501 ggcaatgccg gccgccacct gaccgtcgcg gattgcggta aaaccttcca gcgcgagtgt
     4561 cgacatcgta gacaaaccac cgcatacgcc catgccgagt ccgcgactgg caagttcttt
     4621 ggaacgtgcg ccgaaccggg aagcgccggc caagtaagcg gtcaaccctg catagcagaa
     4681 gcaagccagc atgttggcca caaacgtgcc ggtatggaag ctgccagccg cgggaatctg
     4741 tgcgaacgca tagcggatag cggtgccgat cgcaccgccg cagaagacga ccaacacaat
     4801 atcaagatga atatcgggga gcttgcgcgc cgggggagtg gtcgtgtgcg taatcggccg
     4861 ggcacctgcg gatggggact gcgaatccat aatagccttt cctgaaaccg ctgaatcctg
     4921 ctgaagacac aacgattcca tgcaatatca aatcgtgtgt aggaactatc agcagtgtgc
     4981 ggttcgaaac gagttcaggc gggttccatc gccggttcct tagcctactc gtggcggcga
     5041 caacaaacgg cagcagtatc tcggatattg gcctatcgcg gtggatttcg gcaagagact
     5101 tctgcaatgc cgatgaactt ggttgccgtg aggcgataaa gcacgtagat gaattgcttg
     5161 gttgcttcca tacctcgtgg gtatggctca acatggttta tggctattgg ctaatacaga
     5221 tcgattgtcg tttaatggaa catgaggata acgggcccta atcgctcggt gccggaaaca
     5281 aggagttgtg aatatgtctg aaaccatgaa agccgcgatt ttcgttgagc ctggcaagat
     5341 gaccgtggaa gaagtgccaa aggcggcact tcagcaggat gatgacatcg tgattcgcat
     5401 ggtgcgcacc tgtgtatgcg gctctgattt gtggttcttc cgtgggctga gcggacaggc
     5461 cgcacacagt caggtgggcc acgaagccat tggcgtggtc gaggagactg gcgcggcggt
     5521 cgaatccgtt aagccaggtg atttcgtggt cgtgccattc ccgtacagct gcggcaagtg
     5581 cccggtatgc aaggccggct tcgaatccgt gtgcccgcac gggggctact tctccgcttg
     5641 tcaggccgaa tacctgcgtg tgcccgaggc ggacggcacc gtggtcaagg tgcccggttc
     5701 accggaagat tattccgatg aacagctcgc atcgctgctg accatttccg atgtgatgtc
     5761 caccggttat cacgcggcgg tttctgccga ggtcaagccc ggtgacacag ccgtggtcat
     5821 gggcgatggt gcagtcggcc tgtgcggtgt gattgccgcc aagatgcgtg gtgccacgcg
     5881 cattattgcc atgagccgtc acgaggatcg cgcggcgctc gcccgcgaat tcggtgcgac
     5941 tgatatcgtg cccgagcgtg gccaagaggc tattgacaag gtgctggaga tgaccggtgg
     6001 ctatggtgcg gacgcggtgc tcgaatgcgt gggctccaag cagtccttcg acacggcgat
     6061 tggcctgatt cgccgcggtg gcgtgattgg tcgtgtgggt ctgccgcacg acgtggagat
     6121 cagcgccgaa ggcacgttct acggcaacat cggcatcaag ggcggcccgg ctcccgtgcg
     6181 ccactacgat attgacggtg gtctgctgga tgcggtgctt aaaggcgaga ttaaccctgg
     6241 ccgcgtgttc accgccgaat atgacctcga ccatattcag gacgcttatg aggccatgga
     6301 ccagcgcaaa gttattaagg cgttgatccg gttctaatcg cgtggtgtga cggggtttac
     6361 cgcagtggta tttaacgtcc gtatgtatgc gtcccctttg tagggccgca tacatacgga
     6421 cgttgtgatg tgtctgacgc ggtttacgct gaacgattgt ggtgacgcaa accggtcacc
     6481 tgttactcgg cgagcagtgc cttgtactgt gcagcggact gggcgagggc ctcgtcggtg
     6541 atgccgaaag cgccgcacgt ggcgaacggc ttgagtcagg tcgcgccgat gtataagccg
     6601 tgccctcgat cggaataagc agctgctcga tggggtagtg gccggggtgc cggtctggta
     6661 ggccgattcc ggcgaaccgg tggacactgc gcatcgcgtc gggaaccgtg gccaaggcgt
     6721 acaccgaact cgccgactgc ggcatcatcg acaccggtgc cggccgcgcg gcgcgtggaa
     6781 aacgggtcaa gccgattccc gaaccgctgg tgcgcgcggc caagacctat gccgacgagg
     6841 cccgccggct tggcgcgagc ctcgatgacg ccttcaacgc cctgtgcgcg cagtggtaaa
     6901 gcgcgcaccg atgcgaagcc tacacggggt gcaccgtcag cgaaaagtcg ccgatggtgc
     6961 tgacggtcga gggtgaggcc agattgcaga catctcgcaa gcagtacgtg gcatacagcg
     7021 gaagataggt tgttccgccg tcctgcgcaa tattggagcg ggacagcacc acgccctgcg
     7081 agatgcgata ttcggcattg gccaatacgg cgtcgatggc ggcgtgcgtg cggtagctgc
     7141 gccccgactt gacctcgaac ggcatcacag aatcggcgtt cgtgtcagcg atgaaatcga
     7201 cttcaccatg tttctttgcc atgtagtaat acagcggaat gccttgcgcc gtcagctcct
     7261 gagcaatcac gttctcgtag atggcaccca gattcggtga ggaatcatca aggtatgcgg
     7321 cttgcgcggt cggttgcggg tatcgtgcca tgagcatacc ggtatcagac tggtacagtt
     7381 tgaacttcgg tgattgcgcc gtgcgtttca atggtgtttt cggctcggtg acgcagttgg
     7441 ttttcaatgc cacacccgca tcgacaagcc aaagaaaatc cttctgattg cggtcgtacc
     7501 gagcaccggg gctgatggag gagacggcga atctgccgtt accgtcaatc agctgagtgg
     7561 ggagctggtc gaagatctcc tgcacctgca atgcgcgatt gcctgcgtac tgcgaaatat
     7621 cgtggcgata ttgccggttc aactcgttct gcaccgcgcg cacagccgcc aaatcaccct
     7681 tggtgtccag aaaacgttgc acgacttccg gcatgccgcc gactaccaga taggtgcgga
     7741 agttcgtcat cattgcatcg tgaatgtacc ggtcgattgg nnnnnnnnnn ctcggatgtt
     7801 ttcaagtgtc gacttctgta cgccgatggc ccaacagaat tcctcaaaat ccatgggacg
     7861 catggttttt tcgatgaccg agccaacggg gtaggagcgt acgcccttga actcagtgcc
     7921 gagcatggaa ccggagaaca cataatcgaa acggccatcg gccaccagaa acttgaccat
     7981 ggtcatcaca tccggtgcct cttgcac
//
LOCUS       DAWBMW010000065         7855 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_2158_length_7855_cov_2.783846, whole genome shotgun sequence.
ACCESSION   DAWBMW010000065 DAWBMW010000000
VERSION     DAWBMW010000065.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 7855)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7855)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7855
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2158_length_7855_cov_2.783846"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    276..285
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1395 a         2253 c         2515 g         1682 t
ORIGIN      
        1 cggttggaga tggcgttggt ggcgaactgc agttcgaggg tgaacggcac ggcggtgacc
       61 agcgaggtgg tcttcagcat gccgatggtc tcgttgccgg tgggcgggat gatgatgcgc
      121 atagcctgcg gcagcacgat gcggcgcatg atgagcgtgc ggttcatgcc caatgccttg
      181 gccgcctcgg tctggccggg gtccacggct tcaaggccgg cacgcacgat ttcggacagg
      241 taggcggcct cgttcagagc caaaccaatc catgcnnnnn nnnnnccacg ttggagtcga
      301 tgctccagaa ctccaccgag gtgaacggaa tgccgaggct aatcttggga atcagcacgg
      361 cgaacagacc ccagaaaatg agctgcgtgt acacaggcgt gccgcggaag aaccagatga
      421 agaaccagct gacgccgcgc agcaccggat tgatggattt gcgcatgatg gcgagaatca
      481 ccgacaggat gatcgccacg accatggaga tcacggtgag ctccaaggta tagcgaatgc
      541 cttcaagcac gttctcgttg aacaggtact tccagacggt gggccaatcc agtcgcggat
      601 tggtaatcaa gccttgaatg agcatcgcgg cgagcagcac cacgatcact gcggccacaa
      661 tcgggccggg gcgcttgacc ggcagcgcct tgatgcggtt ggggatgttc actccctcac
      721 cgtcgacttc tttcttcgcc ataatttggt tccttggacg tgttgggcag cctgaactgc
      781 aagttgcaac tagcgttgaa ccttagtgta cgaatggggc gactgtgtta caaacagtgc
      841 gccccattcg aattttcgcg acagcttgtc ggctattcga cggccgggtt gatttcggcc
      901 ttgtccagtg cgccggattc gacgccccag tgctggagga tcttggtgta ggtgccgtcg
      961 tccataagct tttgcatggc cttctgcacg gcttcggtgg tctgggaatc gccctttttg
     1021 atggcgatgg cgttcggcac ttcatcgaag tccttgccga gctgttccag ctgaccatcg
     1081 gtctgggcaa tggcgtagcc gacaaccggg gaatcggcga agaagacgtc ggccttgccg
     1141 gtcaccacag cggtggtggc gtcggtctgc tgcttggagg actggatggt gatgtctttc
     1201 ttgccgtccg ccttgcactg cttggcggtc ttgttgatgg cctcttcctc gacggtgccg
     1261 gtctcgacgg caacgttcag accgcacagg tcgttctcat cgacgttctt ggggttgcct
     1321 gcggccactg catagcccat gcctgcggtg aagtaggaga cgaagtcgac ggattccatg
     1381 cgctccttgg tgatggtgaa ggcggcgatg cccagatcgt acttggagcc gatggccgga
     1441 atgatcgtat cgaagttgga ggagacgatt tcggtatcga gaccgaagat gttgcccatg
     1501 gcgcgggcaa gatccatgtc gtagccgatc tgggtcttgc cgtcggcgtc gaggaattcg
     1561 gccggggcgt aggaggggtt ggtgccgatg gtgagcttgc cgtccttggt cacggattca
     1621 ggcagcagtg cggcgatggt gtcatccttc tccacggagc tgacatcgta gctggtgatg
     1681 gtggcggcat cggagccctt gtcggaatcc gtaccggcat cggccttgtc ggaggtgccg
     1741 caagcggcgg tggcgaacag catggccgag ctcagtgcaa tggccataat ggacttcagt
     1801 ttggtggact gcataatcgt ttcttcttct ctcttacgcc gcttcaacgg caacgatcaa
     1861 cggttaagca tatttataca ctacagtgca tacatataca aaaagactcc cgccgcaggg
     1921 caacgggagt cttcatagta accgaaacta ctcgacggac gggttgatct cggccttgtc
     1981 gacggcgccg gaagagacgc cccacgtgtc gaggatcttc ttgtaggtgc cgtcgtccat
     2041 cagcttctgg atggccttct gcacggcttc ggcggtcttg gtatcgccct tcttgatggc
     2101 cacggcctcg ggcaccccgc cggcgtcttc accgagcgtc tccaaggtgt cgccggtctg
     2161 cttgatggcg taggcggaca ccgtggaatc ggcgtagaag atggaggcct tgccggaagc
     2221 cacggcggtg gtcacatcgg tctgcagctt ggaggactgg atgtcgatgg catccttgcc
     2281 gtccgccttg cactgctcgt tatccttgtt gacttcctct tcctgggtgg taccggtctg
     2341 cacagccacc ttcacgccgc acaggttcga ggagtcgacc ttgttcgggt tgcccttctg
     2401 caccacgaac gtggaaccgg ccttgaagta ggtcacgaag tccacagcct ccatgcgttc
     2461 cttggtcacg gtgaaggagg agatgccgat gtcgtacttg gagccgacgg acgggataat
     2521 cgaatcgaac gtggaggaga cggtgttttc cttcagtccg aacactgcgg ccagcgcctt
     2581 ggacaggtcg acatcaaagc cgaccggggt cttgccgtcc tcggccagga actcggccgg
     2641 ggcataggaa gtatcggtgc cgacggtcag cgtaccgtcg ccggccacgg aatcgggcag
     2701 cagtgcggca atctcgtcgt ccttctggat gctgctggtg tcgaagcctt gaatcgaagt
     2761 gctggacgag tccttggatg aatcgccgct cttggatacg gtgtcggtgc tgtcagtcgt
     2821 gccacaggcc gccacggcga tcagcatggc cgcgctcagg gcggcggccg cagcggattg
     2881 tattcccttg cttagagaca ttggatttcc tctctccgga tagtcagctg ggcgggtatg
     2941 tttcccagat ggttccagag cgtaggagtg caatgctacg gaagcgttaa tcgtgggcga
     3001 aatgacccac aacgcggaca tcctactctt ggctcccctc cggaggggag ccaaggggcg
     3061 ggactagtgg cgcatgttgg cgtagcgcat ggcgcgtagg gcttcgcgct tgtcctgctc
     3121 ttcgcgcagg gcctggcgct tgtcgaattc cttcttgcct cgggccagcg cgatctccgc
     3181 cttgacgcga ccgtccttga agtacaggct cagcggcaca atcgtgtatc ccttggcttc
     3241 gatgccgcgg gcgagcttgg tgatctgcag tcggtgcagc agcagcttgc gcttgcgctt
     3301 gggcgcatgg ttgttccatg tgccgttcag gtattcgggg atgttcgcgc cctccagcca
     3361 catctcacca cggcggtcga tggagatgaa cgcctcggac agcgatgcgc ggccttcgcg
     3421 caacgacttg acctcggtgc cggtcagggc cagccctgcc tcgtacttgt cttcgatggc
     3481 atagtcatgc cgggccttct tgttctggac gatgagtttc tcaccggttt ccttgggcat
     3541 gtctcctcct ttcgtgggca ataactcgtg agtagcaact cgtgggtagc aactcgtggg
     3601 tagcaacgca acatccgacg atatcacgga ccggcctcga atacgaaaaa gggttcctct
     3661 tcaagaggaa cccttcaacc gtatatatcg gccgaaccaa ccgatcagtg gacgaaccag
     3721 tactgaccca aattggtcaa ggtgcggtag tgcatgatcc acggaccacc ccagttcatt
     3781 tcagaaatct ggaatgtgcc gtcgccatac acggcttcga ccacggcaac atggccatag
     3841 gtaccatcgg caccgtcctg accaggcaag aaggagagcg ccgcaccgac ctgagggttg
     3901 tggtccacgc gcaagccgta cgagggggcg ctgcgccacc agtaaccgcc attgccgaga
     3961 taggacggcg tgccaatgcc catctgctta cggcgttcat acgcccaata cgtgcactgg
     4021 ccggtggcat aggcgttgcc gtaatcaccg ttcgatgtac cttggccgct gctgttgccg
     4081 ccaccgctgg gaaccgtcgg ggtgggggcc ggagccggag ccggagccgg cgccggagcg
     4141 ggcgccggag cgggggtcac cggagtagac ggcgtaccgc cctggttacc ggtatccacc
     4201 ctgttggcgg cgtcctgctg ctccttctgg aactgacgat tgtaggaatc gacctgggaa
     4261 gcgatcagta cggtctgggc cgcctgcttg gcggattggg aatccagact gtcgatcatg
     4321 gaagacagtt cgttacgcct tgcttcgcct tcctgacgta gcttgtccaa agcgtcacgt
     4381 tcgctttgcg cggtttcggc ggcggtctgc gcggaagcgg ccttctcatc ggcctgggtc
     4441 ttgagcacag cgatctgctt ttcgatggcc gacagacgtt cgccacggtt cttcgacgtg
     4501 ctcagcgagt tcgcggcgga ggaagccgca ttcgcctcgt tgcgggacaa cgcgtcccgg
     4561 gactgcatgg agttgacgaa gtcctgtgtg ctggtggcac ccgtgaccac gctcatcacg
     4621 tcggaagcat tcgagccgtg catctcgtca cgggccagct gggccacggc ggcgtgcgcg
     4681 tcatcgtagt ccttgccggt ctgcttgatc tgctcctcga gcgtctcctt gtctttctgt
     4741 gcggcggaaa gacgttcgga tgcggcgtcg gcctcgtcct gagcggtggc ggcatcctcg
     4801 ttggcctgcg tgaccttgtt ctgggcggcg acaatcttgt tgttcgtcaa atcatccagc
     4861 tcaagtacct tatccgccag ttcctggttg acaccggcca actgctgctt gagttcggcc
     4921 tcgcgctgta ccgacgcggc gtgctggttc tgcgcattga tcaggtcgga ataggtgtct
     4981 gcctgggcag gttgggacga gccgaacgcc agacctccca cacaggctgc ggcggccacc
     5041 aatgccgcca gtgcgggctt gatcacacgt acattcgtca tatgtgaatg gtcctttcgc
     5101 tcacttaagc caataccata acatgcgaac ctgagaggct tgcacacgat gtcatcacat
     5161 ttctcgcggg taagccggtc gcgttacgcc ctcaaataac gccgcaacga aatggttgaa
     5221 gcgatgatgg acagcagtat cgcgcccaca accaggaacg gcgatatcac cagtaccgtg
     5281 agctgattca cataaggaat ccatgtaatg gatttcgcca gccagccggt gatgaacacg
     5341 ttcacaatgg ccgaaagcat caggcacgag agcaccgagc cgatcagtga ggcgatggcg
     5401 ccctccagaa tgaacggcag acgaatcgtc cagttcgagg cacccacgta tctcatgatt
     5461 tcggtttcgg ttcgtctgga cgcggcggac atgcgaatgg tggttccggt cagcaggatg
     5521 gcgaccacca ccatcacgcc ggcgagcacg gccgtaaccg ccgtggctcg gttgagcacg
     5581 gcgaacaccg ggtcgaagat ctggcgctgg tcggtgacgt cttccacgcc ctccttgccg
     5641 gagagcactt cggagaccac ctgatatttg gtcggatcct tcagcttgag ccagagagag
     5701 tcctgcatat cgtccgcggt cagcgtacgg ccttggaatt cgccattcgg gtactgcttg
     5761 gtgaatgtgc tgtcgtagaa gtcctgcttg ctgacgtagt caatgttgga gaccacgtcg
     5821 ttgagttcgt cgcgaatggt tttctgcagt gccgtgatct cattggctga gggcgatttg
     5881 ccggaagcgc agttcgcgga ttggctggtg ccgtcggggc atagccacac cacgacctcg
     5941 accttgtcgt accagtcgcc cttggctttg gtgatctgcg cctgagtaag caccgaagcg
     6001 ccgatgaaca ggaatgagat gaaggtgacc agcgtcagcg acagcagcat cgacaggtta
     6061 cgggtgaggt tggcccacgt ttcggacaga atgaatcgtg ctcgcatatg atccccctta
     6121 ctccttcgcg ccctcagcgg gttgatcgtc ggatgtttgg tctgcggcgg actgctcgcc
     6181 gacaggcggt acgggcggtt ccggcggctc ggcggcggat gacacggacg tatcggcaac
     6241 cgcggcggtc tcgccggact cgtcattgaa cgggtcgaac gcctgggtgc tttccagggc
     6301 ggcggcctgc tcgtccaacg ggatgccctt gccccaggtc agcgtgtctt ccagcggcgc
     6361 gaacgtctcg ccatagcggc cggtgcggcc ggaatgcacg gagttggcca gacgggcgat
     6421 gccttcggac ggctcgtcac tgccctcgat ggccttggtc gccgccgcca gcacatgatg
     6481 ggcggcgtcg gacttggctt cgacttcggc atccgggaag aacagggcgg aatcatagga
     6541 gccatgagcc tcgtcacgca cgatcttgcc gttgtgcagt tcgaccacgc gcttgcgcat
     6601 cgagttgacg atctcttcgt tatgcgtggc catgacgatg gtggtgccgg tgcggttgat
     6661 ggcgtccagc acttccatga tgcccagcga agtggtcggg tcgaggttgc cggtgggctc
     6721 gtcggccagc agaatctgcg ggtggttcac gtaggcgcgg gcgatggcca cacgctgctg
     6781 ctcgccaccg gagagctcat gcgggtagtt cttttccttg ccggtgagcc ccacggtatc
     6841 cagcaccttg ggcaccaacg atttgatggt ggaccgccgt gtgccgatca cctcaagggc
     6901 gaacgccacg ttctcccaca cggttttgtt attgagcagc ttgtaatcct ggaacacgaa
     6961 gcccagcgaa cggcggtact gcggcacttg acggttggtg atgcgccgca gatcgttgcc
     7021 ggccacgcga atctcgccgt tggtggcctc ttcctcgcgc aacagcaggc tcaagagcgt
     7081 ggtcttgccg gagccggacg cacccacaag gaacacgaaa tcaccgcgtt cgatatcgag
     7141 gttgatgtcg tcaagcgccg gtcgggtgcc tttgggatag atcttggaca cattgtccag
     7201 tgagatcagt gccataatgc tgtttactcc tcggtttcgg cggcgcggcg ctgttcatgg
     7261 cgccagcgga tgccggcctc gatgaacgcg ttgatgtcgc cgtcgaacac ggcctgagtc
     7321 tggctggttt cgtagccggt gcgcaggtcc ttgaccatct ggtaggggtg cagcacgtag
     7381 gagcgcatct gatcgcccca gctggccttg atgtcgccgg cgagctcctt cttcttcttg
     7441 gcctcctcct cgtggcgcag cacgagcagt cgggactgga gcacggccat ggcggaggca
     7501 cggttctgaa tctgcgaacg ctcatcctgc atggtcacca cgatgccggt gggaaggtgg
     7561 gtgatgcgca cggccgagta cgtggtgttc acgccctggc cgccggggcc ggaggaacag
     7621 taggtgtcca cgcggatgtc cgtatccgga atgtcgatat ggtcggtggc ctcgaccagc
     7681 ggcaccactt ccacggcggc gaagctggtc tggcggcggc cctggttgtc gaacggggag
     7741 atgcgcacca gacggtgggt gccgccttcc acggacagtc ggccgtaagc gtagggggcg
     7801 tccacctgga aggtggcgga cttgatgccc gcctcttcgg cgtaggaggt gtcca
//
LOCUS       DAWBMW010000066         7830 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_2166_length_7830_cov_3.215563, whole genome shotgun sequence.
ACCESSION   DAWBMW010000066 DAWBMW010000000
VERSION     DAWBMW010000066.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 7830)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7830)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7830
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2166_length_7830_cov_3.215563"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3290..3299
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4067..4076
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1491 a         2523 c         2207 g         1589 t
ORIGIN      
        1 tggccggcat ctaccgtccg cgcccgggtc tgccgttcac ccactacaag ttcgccggca
       61 agacgcgcct ggtctgggtg agcttcaccc cgcagcaggt tgatattgac accgattccg
      121 acaagggctg ggaatacctc atgtccatct tcgatcagat ggccgcctcc catgtaagct
      181 acatccgcct cgacgccgtc ggctacggcg ctaaggaagc tggcacgagc tgcttcatga
      241 ccccgaagac gttcaagctg atctcccgcc tacgtgagga aggcgtcaag cgaggcctcg
      301 aaattctcat tgaggtgcat agctactaca agaagcaggt tgaaatcgcc tccaaggtgg
      361 accgcgtcta cgacttcgcc ctgcctccgc tgctcctgca ctcgctgttc accggtcacg
      421 tggaacccgt ggtccactgg accgaaatcc gcccgaacaa cgccgtcacc gtactcgata
      481 cgcacgacgg catcggcgtg atcgacatcg gctccgatca gctcgaccgc agcctcaagg
      541 gcctcgtgcc cgacgaggac gtcgataatc tggtcaacac catccacgcc aacacccacg
      601 gcgaatccca ggccgccacc ggtgccgccg ccagcaacct cgacctctat caggtcaact
      661 ccacgtacta ctcggccctc ggctgcaacg accagcacta cttggccgcc cgcgccgtgc
      721 agttcttcct gccgggcgtg ccgcaggtct actacgtggg cgcgctcgcc ggccgcaacg
      781 acatggaact gctgcgccgc accaacaacg gccggcacgt ccacggccgc cctcaccttc
      841 gagcccggac gcggcctcgg cacagacaac accaccccgg ttgccagcct tgcctggagc
      901 gatgccgccg gcgaccacga aacccgcgat ctgctcgcca acccgccgat tgccgatatc
      961 gactaaccgt tggcccataa acgccactcc gctgtgcgcg ctccaagtag tgcgcccggc
     1021 gtaagctggg ttcatggaga gcaaaccgcc cgcagtgcac tgaagccagt gcgcccgggc
     1081 ggttttgcgt atgcggggtt gaaggtcatg ctcctgcggg cgcggcccaa gcatcccgcc
     1141 ggaatcgacg agacgagcag cagaacagag aaagagcagc atcatgttgg aaccgtatgt
     1201 gcgtccgggt attgatgatc gcccggattt ggacaaggtc ctaagtcccg aagacaaagc
     1261 cgccgtggcc agactggcca tcaagaatcg ccgccgcccg cccgaagcct ctgcggacca
     1321 gcctgaggcc caggctgtgc gattggcttc cgccggttct tcggcagccg ctgctgcagc
     1381 cgccagtgtg gacactcccg cgccggatat gtcgtccgcc tacctcgaca tgcgcgatcc
     1441 catggtcgcc gtcaatggcc aacgccctac cgcaggtcag gtctcccgac tcaactgggg
     1501 tttcttcgct gcttcaattc tggtcggcgc accttgggtg gcgttgaaca ccattgccat
     1561 gcccaacgcg attgcgcgta cgttcggcta tgacactgct gttgccggtc atatcgccat
     1621 caatccggcc actggccgcc cgttgcccgt cgccaccgaa ttggcgatgc ccctggccgt
     1681 gttggtgatt gtcggcgtag tggcctccat gtttgcgatg ccgctgattt cagtgttgtc
     1741 cgaccgcaca cgcacgccat tgggccgtcg taccccgtgg atggttggcg gaggtctgct
     1801 gtgcgcgctg atcacgctga ttctggggca gaatatcggc atcattgtgc tttgtatttt
     1861 ctgggtgttc ctacagttcg cctatgcgat gctttccgtg ccgttgacca gcgccatcag
     1921 cgaacgggtg cccgacaagt tccgccctcg catcgaacgc tggcatggca tcggtgtgat
     1981 gctcggccaa gcgcttggcg tgtgcatggg tgcgcttggt gtgatgttca attcgtttgc
     2041 gccgtttagt tacaccgccg tgctatttgc agtgtccggt attgccaccg tattgattct
     2101 gcccaaggag ccttctagcg ctgagcagcc gaaccagctt tttgaccgca gtcaggtact
     2161 tgaccaattg cgcccgccgg cccacgctcc cgagttctcc cgggtgttcg ccgcccgtac
     2221 gtgcatgatg gccggtgttg ggctgaccgg tgtgtttctg tggtatctcg tacgtttctg
     2281 ggtttatggc aaggccgtgg tacttacgtc cgcgccgctg accattccgg ccggattcct
     2341 cattgccggt atggctgtgg ccacattgat cggtgccgcg ctcgcctcct gggccgccgg
     2401 gccgatttcc gagcgaatcg aggaaaagaa cattcccgtg gcccgtgtgg tggccggtgc
     2461 ctgcctgctg tatgccgctg gtttggcgct cgcatggggc cttggcgtct ggtccaccgg
     2521 cactgcccgt gaaaacggca tgctgctgtt tgcgttgatt tccggattcg cctttggcgt
     2581 ctatgattcg ctcggacttg aactggtgat ggactctttg cccgacccgc gccgagccgg
     2641 ccacgacctc ggcatttatg cgctcgccaa ctcggccggt ctggcgctcg ccgccatcat
     2701 cggcgcgcta ctggtgaacg ccttcgagca tagcttcggc tattacctgc tcttcccgtc
     2761 cgccatcgtc atggtgctgc tggccggaat cgtcaccctc accaccaaat ctgccgaata
     2821 ataaaccggt tcgagtacac tggtcagcag tgatccagtc cagtactcgc aggggagcaa
     2881 aaccatggtc ggcatgcgcg acgtagccaa agcggcaggg gtgtccttaa gcaccgtttc
     2941 gttggtggtc aacaacaccg gctacgtctc ggccgatatg cgtgccaaag tcgagtccgc
     3001 gatgcgccag ctcaactaca ttcccaacga gctggcccgc aacctctacc gaaaccgcac
     3061 caacaccatc ggcgtgatca tgccgaccat cgcccacccg ttcttctcca cactcaccgc
     3121 gcatctgcag cgcgagttcg ccgcgcgcga cctcaaaacc ctgctctgct ccaccgccga
     3181 tacagacaag ggcgagagcg agtacatcga catgctccag cggcacatga tggacggcat
     3241 tatcatggcg gcccacaccg cgcatccggc cgactactgg acctccatcn nnnnnnnnnc
     3301 gctcggtgag ggtgtgccgg tcgtccggtc tgatcatgaa cagggaggcc gtctgattgc
     3361 gcgccagctc atcgattccg gcgcgcgcca cgtggtgctc gttggtggcc cgcgttccca
     3421 gttcgcggaa ggcaccactt tccccacgat tcgctaccat ctcaccgtcg agcgtctgct
     3481 ggctgaggcc ggcattgcct gcgattatgt cgaagccggc accgtggccg acatcgccgc
     3541 ccacgaagcc accgcatgcg ccattttcga acagtatcct gatgtcgacg ccatcgtcgg
     3601 ctccgatgtg gtggcatccg tggcattgca ggaagcctca cgacggggta tcgaggtgcc
     3661 tgctgatctg caagtcatcg cctatgacgg cactctcatg gccgaaaccg ccggcaaacg
     3721 cctgaccgcg attcgacagg acttcccggc catcgccgcc gcgctcgccg tccgcatgga
     3781 ctcacagatc gccgccgacg gtggccccgc ctctccgtcc aagaacgata gcgcttcgga
     3841 cgttgacacc caacccactc ccgaagaagt catccccgta gccctcatcc ccggcgaaac
     3901 cacccggtga ctcctatctt ggctcccctc tctgaggctg agctgtgaag caaacagtcc
     3961 ggtggactgt ttgtaggcga aggcgagccg ggaggcgagc aggcagactg cgggcggtag
     4021 cccgtctgta attgcggccg agctggcggc gtaggcgact gaggggnnnn nnnnnnccta
     4081 tcttggctcc cctctctgag aggagccggc ggcgtagccg cccacagtca cccatctcac
     4141 atgactccta cgtcacaatg tccacaatgt gaacccttgc tggattggca agacatacaa
     4201 acctatgttt acattccagt tcaggtcgaa cggcctgagg aaataccgaa aggaggccac
     4261 gcgatgactg gattcaatgc ttctgcgttt gtatccgcac gaattattag cccgtcttcg
     4321 ctggcggact aattcggcac tgatgcttga agccccgtca acgaaggcag ggccaatcaa
     4381 gctgacacaa tcggcacggc cctgcatgat gcggggcttt ttttgttgcg ctcacaaggc
     4441 ttacggcaga atcccgcaga cggaaacggt agcggacagt acacggcaac aacacagcga
     4501 cagaacaaca acctccctgt ggggcgaaat aatcgcccgc cgatgagagt gaacgagagc
     4561 aaaacaggag taacaatgac tgcagcagtc gaagagactg gcttagggca ggcggcaggt
     4621 aaagccgcta ccttggtacg cgattcggtc aagaccgtga tcgccgcgtc gatggtcggt
     4681 accgccatcg aattctatga cttctacgcc tacggcaccg ccgcggcaaa ctacttcccg
     4741 cacatcttct tctccgcgaa ggacaacccc accgtcgcac tgctgatgag cttgctgacc
     4801 ttcgccgtcg ccttcatcgc ccgcccgctg ggctcgctga tcttcggcca cttcggcgat
     4861 cgcatgggcc gcaagaccac gctggtcgtc agcctgatga ccatgggtgt cggcaccttc
     4921 ctgattggct gcctgcccac ctacgatcag gtcggcgttg gtgcagtcgc catcctctgc
     4981 ctgctgcgtt tcatccaggg catcggcctg ggcggcgaat ggtccggcgc ggcactggtc
     5041 gccaccgaga acgcgcctga agacaagcgc gcactgtacg gctccttccc ggagctcggc
     5101 gccccgatcg gcttcttcct gtccaacggc acctacttcg tgctcgaatc cttcaacaat
     5161 caggacgcga tgctggcttg gggctggcgt gtgccgttcc tgctgtccgc catcctggtg
     5221 atcgtcggtc tcgtggtccg cgttcacatg gaggagaccc cgatcttccg tatggctcag
     5281 gagcagaaga aggttgtcaa gtccccgctg accgaggtct tccgcaagtc ttggcgtcag
     5341 gtgctgcagg ccaccttcct tgtggctgtg acctacacgc tgttctacac gctcgccacc
     5401 tggtccctcg cttggggcac caaggagcag ggcgaaggcg gcggcaacct tggcttcacc
     5461 aaccaggaat acctgctcat gctcatgatc tccatctgcg tgttcgccct gttcatcgtg
     5521 ctgtcctgcc tgtacgcgga caagatcggc cgccgtcgcg tgctgacctt ctcctcctgc
     5581 gcacttgtgg tgttcgccgt actcttcccg ttcctgctgg acggtggtct ggtcggccag
     5641 aagaacttct tcgccaacat ggtgttcctg tgcgtcggct ttgcctctac cttgccgtga
     5701 tggccgtgtg ctgcctcgtc tccgtgctca cctgcaagga gactaaggac gttgacttta
     5761 ccaagtgacg atgttcacaa cgtgaaaacc cctaattcca ctaagtgaac ccctacaggg
     5821 cttttccgat gcgacgggac taaacctgca tatcggaaaa gcccacaagg aaagccacaa
     5881 cagaatatcg ctcagcgaaa ccccgcagaa cgcaagaaaa attcaacccc ataaataaac
     5941 aaataaataa aaggaggcca aaaatggcag caactatctg gtacgagaag gacgccgatc
     6001 tgtccgtgtt cgatggcaag aaggtggcta tcctgggcta cggttctcag ggtcacgccc
     6061 acgcgctgaa cctgcgtgac tccggtgtcg acgtggtcgt cggcctgcgt cctacctcca
     6121 agtccgtgga gttcgccaag gagcagggcc ttgaggtcaa accggttggt gaggccgtcg
     6181 ctgaggctga cgttgtgatg attcttctgc ctgaccagta ccaggcagcc gtctacaaga
     6241 aggacgttga gccgaacctg aagccgggcg ctgccctggc cttcgcccac ggcttcaaca
     6301 tccactacgg ttacatcaag ccgtctgagg atcacccggt gttcatggtt gccccgaagg
     6361 gcccgggcca catcgttcgt cgtgagtacg ccgctggccg tggtgtcccg gtcgtcgttg
     6421 ctgttgagca ggacccggac ggcaagacct ggccgctgtg cctggcttac gccaaggctc
     6481 tgggcgctct gcgtgcaggc gccatcaaga ccaccttcac cgaggagacc gagaccgatc
     6541 tgttcggtga gcaggacgtc ctcatgggcg gcatcaacca cctgtgcgac ctcggcttcg
     6601 acgtgctgac cgaggctggc taccagccgg aaatcgccta cttcgaggtg ttccacgagc
     6661 tgaagatgct ggtcgacctc gctaacgagg gtggtctgaa caaggctcgt tggtcctgct
     6721 ctgacaccgc tcagtacggc gactacacct ccaccgtcat caccgaggag accaagaagc
     6781 gcatgcagta tcagctcaag cgcattcagg atggctcctt cgccaaggag ttcatggatg
     6841 accaggctgc tggcgccccg aagttcaaga agctgcagga agagtactct cacccgcacc
     6901 tcgagaccgt tggcccgaag ctgcgcgcca tgttctcctg gaataacgcc gaggccaagg
     6961 acaaggatga gaccgagtcc ttcaacggca agattgctcg tacccaggtt cagtgattgt
     7021 ctcccctgaa ggggatttaa gctagcaaac cgccgttccc gcccatacgg gagcggcggt
     7081 tttgtgttgt tgcgcgcagc gaatcattct caataacaac tatgcgctga tttcttgcag
     7141 gcaaacgaac accgaatatg caaggtcgtt gatatctcag cctttcccca tatgtgggat
     7201 tgtgcgattc tgttcaaaag ggtgcagagt tgttatcgag aatcgttctc tgcacacggc
     7261 tgtccatact ccaatagtga cctagctcac gttgtggact ccagcggggc tgcgacggcg
     7321 ttctcggtag gattgaactc ggtttgaagg acacaatcct cgcaaaacag gacttatctg
     7381 cacaccgtag atacccccgg cgcccagcgg cgccacaacc cactactgaa aaggagtgcc
     7441 aataatggca gcacagatct ggtatgagaa cgacggtgat ctctctgttc tcgaaggcaa
     7501 gaaggtcgca atcatcggct acggctccca gggccacgcc cacgcgctga accttcgcga
     7561 ttccggtgtc gacgtggtcg tcggcctgcg tccgacctcc aagtcggtcg acttcgccaa
     7621 ggagcagggc ctcgaggtca agtccgtggc cgaggcttcc gccgaggccg acgtgatcat
     7681 gattctggcc cccgaccagt accagcgcac catctgggcc aacgacatcg agcccaacat
     7741 caagccgggt gctgctgttg cgttcgctca tggcttcaac attcactacg gttacatcaa
     7801 gccgtctgag gatcacccgg tgttcatggt
//
LOCUS       DAWBMW010000067         7824 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_2168_length_7824_cov_2.792251, whole genome shotgun sequence.
ACCESSION   DAWBMW010000067 DAWBMW010000000
VERSION     DAWBMW010000067.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 7824)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7824)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7824
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2168_length_7824_cov_2.792251"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6034..6043
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6963..6976
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1478 a         2567 c         2374 g         1381 t
ORIGIN      
        1 ttcgagggcc gctctgcgtt gaggttccgg caacctgaca gaccaatgtt cgagctgttc
       61 tgcctctacc gtaactcata ttgacaacga ttgcaataca ttcggtgagt tggacgaggc
      121 tagaggtgtt ggtgtttgtc gtgatggccc gttgactatc cccagtcgac tttgccgaca
      181 gcctcgccgg cggaggtgag gtcgaacggg tttaaccaaa cacgacgaac gattgcgggg
      241 agagcgtcag ctgcccgccg ctcacggccg gttcaccaag actgagcagc acgtcggcgt
      301 cggaagagag ctcgtccggc aggtattccg tgaggaatga ggcgattgag gcacctgaat
      361 cggcgttgaa ataactggaa tcgccttcgt gctccatagg cgtgtcggta ttgcgcagca
      421 acaggttgta gccgttcggg tggccgtccc ggcgccagtc gaggtagagg tccatgtcga
      481 agccggcctg catcgattcg ttcggatggc ctggacgcca tcggcggacc gacctgggtg
      541 gattccgata aggcggccgt atgggtgacc aactgggaca ccgagcgcaa cggctcctcg
      601 ctcaccaccc gtgacggcac cagctacctg ctcgccaacg ccttcatgct cgcctacgat
      661 tacggccagc cgcacatctt ctccggctac tactacgccg gcgtcgatga cggcgctccc
      721 ggcgccaccc gcacctccgt gccggacatg acctgtccga ccgacggcat cgaaaccgcc
      781 ggcacctggc actgcgcgca acgctggacc gccatccgcg gcatgatcgg cttccgcaac
      841 gccgtggccg gcacccggaa atacgatccg tccgatcccg cctggttcac cggcgaaaca
      901 ccgcataagg gcgatgacac cgccgccaag tccgccgatt ccgcagcgtc cgccaagtca
      961 ggcgacgcca agtccggaaa ggcaacgtcc gatgccatcc gccgcgccgc aacgctcgcc
     1021 gccatcctcc tgcccgtcat cgtcggcgtc acgttcatgg cgatagctcg caggaacggc
     1081 aaagcccagt ccgacaacag cggcccgaat aacgactgat tcggccccgc tcagccgcgc
     1141 acccgcggcg cggcggtgga gtcgcgtacc agcagttgca cggggaagat ctcgtgcggc
     1201 tggtccaggg tgcgcccctc gataaggtcc agggccttgc gcgcggcggc gacggccatg
     1261 tcgtacggct tctggcgcac ggtggtcagc cccacctcgc ccgagaacgt gctgttgtcg
     1321 aatccggtga cggacaggtc ggtcggaatc gccatgccgc attggcgcag gcggaagaac
     1381 agcggaatcg ccagcgaatc ctgatggaag cacagggcag tcggggcgtc gtcgcgcgcg
     1441 agcagcgcgg tgagggcggc gtcgaccgga tcgtcgccgg cctgcatgtc catcgtctgg
     1501 ccggtcacgc cgtccagttc gctgcacgcc tccaggaatc cggtgacgcg gttgtgcgag
     1561 ctgaagccca tcgtggcatc gaacgtctcg tacaaataca acaggttgcg gtggccgagc
     1621 gtggtcagat ggcgcacgat cagccggatg ccggccttgt cgtcgatgcc cacggaggcg
     1681 gtgaaccctt gcgaggccgt gttgatgccg atgagcggga tccgcgtggt gttgagtcgc
     1741 ctgatttcgg ccggggtgat ggcgaacgac gagacgatga ccgcgtccgc gttgccgcgc
     1801 accggcagtt cgtcgaagaa ctcctcgcgc gcgcccgcgt cgccgatcgg ttggatgacc
     1861 agatcgtagc cggccgatcg cagcacttcg ttgaggcctt cgatgatgcg cgaggtgaac
     1921 cagtcgagct tgctgctgcc caccagcagc gccacgcggt gggagcggcc cgatttgagg
     1981 atgccggcca tgcgggatac cgagaagtcg agttgttggg cggcctccat caccttggcg
     2041 cgcgttttgg cggagaccat gtcagggcgg gagaacgcgc gggagaccgt ggcggtggag
     2101 acgcctgctt gcctggcgac gtcctcgatg ctggctttca tggcccctcc cggtttttca
     2161 aagttgacgg tgtgacaaac ttctcttcat tgtaaatcat tgcagtacgt tttacgacag
     2221 ccataatgca aggggttgcg atattgcgac acgccgaaaa tgtagaccaa tatggtctca
     2281 aaagctttgg aatgtaacgg gttgcaaatg taacggtttg catggctgag aaagctgggt
     2341 tgctgaacta gttcgacacc cgtatactga accgtgttca cttagagctt gcgaggaaat
     2401 aagggagatg atgaagatca agaaatccct cgccgcgctg actgcaaccg tgagcattat
     2461 ggcacttgca accggttgca gttcgagcgg ttcgtcaggc gtcgtcgaca cccaggccga
     2521 caagggcctg gccgtcatgg gcgacacatc acctacgacc ccaaccactt ggtcaacgac
     2581 ggccagccca tcgaactggg ctactggtcc tggggtgacg aaagcactga cccggtgatc
     2641 tccatgctcc gcgagtacga gaagatccac ccgaacgtca ccttcaagat ctcgcaggtg
     2701 gcttggagcg actactgggt caagaccccg ctctcgctca agggcaacaa gaacgccccc
     2761 gtgctgttca acgtccacaa ctccgaggat gccctgctcc gcccgcacgc cgtccgcctc
     2821 gacggcgcca gcgactggcg catcttccac caggtggtgc tgccgatgag ccgcaattcc
     2881 atcgccgcgc tcgccatctt caccttcctg tggaactggg aggactacct ctggccgttc
     2941 ctcatgatca ccgatgacga caagcagctg ttgtcggtgg gcctcaagat gttctccggc
     3001 cagtacggca ccgattacgg cggactgttc gccgccacct ccgtgaccat catcccggtg
     3061 gtcatcgtct acctggtgtt ccagaagcag ttcatcgccg gcatcgccac cggctctggc
     3121 aagtaaaccc ggccggtttc ggccgaatcc gaaccgcgcc gcagtctccc accagtcccg
     3181 gggagccgcg gccgcatcac tcgtatcatc gtgaataacc gtacagaagg ggtaaccatg
     3241 gcacagtcac cacaactcaa gcagtcgtct ccgacctcgc ccgatccgca ggacttccgc
     3301 ctcgacgcca cgccggccat gcgcgccgac aatgtggtgt ccggcgagca ctggcgcatc
     3361 ggactcatca ccgattcgct cgtgcgattc gaatggtccg attccggcgt attcgagaac
     3421 cgcccgaccc agaccgtcct gaaccgcgat ttcggtagcc cagtcgaacg ccgcgtcacc
     3481 gagcgtgacg gccgcgtcat catcgacacc gcggccctga ccatcgtcta cgaccagcag
     3541 ccgttcagca aggaaggcct gagcgtggtc gtcaaaggcg tggccgacac ccagttcaac
     3601 acctggcatt acggcgacgc ccagcgcggc aacctcaaag gcaccgcccg cacgctggac
     3661 gaggcggacg gcgccatcga gcttgacaac ggcgtgatct cgcgcgacgg ctgggccgtc
     3721 atcgacgatt ccgccgccaa catcatcatc gaaaccgaca cggtgaacgg caaggcgaac
     3781 ccgttcggca cctgggtatc cccgcgcgcc accgccgaaa cggacttgta cttcttcggc
     3841 tatggccacc gctacatcga ggccgtacgc gacttctatc gcctgaccgg ccccacgccg
     3901 ctcctgccgc gcttcgccat gggcaactgg tggagccgct actaccggta cacgcaggac
     3961 ggctatctgg ccctgatgga ccgcttcaag cgcgagggca tcccgttcac cacctccgtg
     4021 atcgacatgg actggcatcg cgtggacgac gtggacccga agtacggttc cggctggacc
     4081 ggctactcgt ggaaccgcga actgttcccc gacccgccgg ccttcctcgc cgacctgcac
     4141 cggcgcggcc tgcgcaccac actgaacgtc cacccgcgcg acggcgtgcg cgcgttcgag
     4201 gacgcctacc ccgaagtcgc caagcgcgtc ggcatcgacc cggccaccga ggagaacgtg
     4261 gaattcgacc tcaccaaccc ggacttcgtg gacgcctatt tcgacatgca ccaccgcatg
     4321 gaggccgaag gcgtcgactt ctggtggctg gactggcagc agggcggcgt gacccgccag
     4381 aaaggtctcg ccccgctgtg gatgctcaac cacatgcact acctcgactc agggcgcggc
     4441 ggcaactggc cgctcacctt ctcccggtac gccggccccg gctcccaccg ctacccggtc
     4501 ggcttctccg gagacacgat cgtgacctgg gaatcgctcg ccttccagcc gcagttcacc
     4561 gccaccgctt ccaacatcgg gtatggctgg tggagccacg acatcggcgg ccacatgttc
     4621 ggctaccgca acgaagagct ggaggcccgc tggtaccagc tcggcgcgtt cagcccgatc
     4681 aaccgactgc attcgtccaa ctcgccgttc tccggcaagg agccgtggaa cttcaaccgt
     4741 gacgtgagcg ccgcgatggt ggacgcgctg cgtctgcgcc acgcgatgat gccgtacttg
     4801 tacaccatga actaccgtgc cgccgaagcc ggccgtccgc tggtcgagcc gatgtactgg
     4861 cagaacccgg atacgccgga cgcctacgag gtgccggacg agttccgctt cggcaccgag
     4921 ctcgtggtcg cgccgatcgt ctccccggac gatgccgccg cctgccgcgg ccgcgccgcc
     4981 gcctggctgc cgcaggggga gtggttcgac ttcttcgacg gccgccgata cgtctcatcc
     5041 gacgccgccg gccgccgact ggaggtctgg cgctcgctcg accgcacgcc ggtgttcgcc
     5101 aaggccggcg cgatcgtgcc gttgcaggac gtcgcggaat ccggcgaagc gatcaactct
     5161 atcgccaacc cgcaggccct gcgcgtgctg gtgttccctg gtgccgacgg ctcgttcgtc
     5221 atgcgcgagg atcgcggcac gtggggcgcc accagcgccg acaccgcgat agcgttcacg
     5281 tggggcgggg ccgatgcgtc gcccagcgcc ttcaccgtcg cgccggttac cggcgatacg
     5341 tccgccgtac ccgagtcgcg cgactggacg gtcgtgttcc gcggcgtcgc gccggtcgat
     5401 gcggccagcg gcgtgcgtgc atggtccggc gaggctccgg tcgaggcaac ggtggcgtac
     5461 gacgaggcga ccatgagcct gaccgtgtcc gtcaccggca tcagctcggc tgccagtctg
     5521 cgcatcgaga ttcccggcgg tctgcgcatc gccgacaatc cggtcgaatc ggacgcgatg
     5581 gacctactgc tgcacgccca gatgctctac cgcaccaagg agctcgcgct gcaggcggtc
     5641 cacaagctcg gcatcggcgc gatcggcgcc ctgcgcacca tgaaccgtgg tccgcgctac
     5701 gccaacgatt tctggatcac cgacatgccc gatgcggtgg caggcgcgct cgaagaaatt
     5761 ctgctgcgca gctgagccca gttgcagttt tctgcaacat ccgggtgctc cccggtatgc
     5821 ggcgataagt tcggcatggt tgccgaagag cgtggcatac cgggtgcagt gcgtgggatg
     5881 gccttcgcga ttgcactcat cgtgcggtca gtggaaagga acctttcatg cacaaggtga
     5941 aacagcacaa gggcgtcgcg gtggccgccg cagtctgcgc ggtcgcgatg gcggcctcgc
     6001 tgttcggcag gacctacggc acggattacc tgtnnnnnnn nnnacccacg ccggcgcggc
     6061 cggggaccgg cgttattgac tggctggcgt tatcgagtga ttggcggtat caattagccg
     6121 gcgttattga tgagccggtg ttgtcgatta gccagcgtta tcgaccggcg ctgaagcatg
     6181 cagtacccac tgcccgcgtt tctcccgcac atcccaatcc ccgtgcgcat gcagcacacg
     6241 ctcccgatag tacgcaagcc cggaatgccg cccctcaagc gtcgcgtcac cttgacgcgg
     6301 acggtccgtc acatcaatgg tgacgcggtc ctgagcggcg gaaaccagca ccacgtaacg
     6361 ctgcgtcgaa tcggcatacc tggcgatatt gccaaacaat tcccgcagaa aatcggcgac
     6421 aaacactgtg tctgagttat tcagcgcatc gaggccccca gcgacaataa cgtcgccgac
     6481 gcaccccatg cgccgcaatc gcgcgccgcc gctggcaagt ctgatggcaa ggctgatggc
     6541 gcgccccagt caccccttgc agtttttctg tataagtgga ttttggctcc aaacgcacca
     6601 ctccgtcatc gccgtcagtc actcccgcaa aaacttgagc caaacatact caactttttg
     6661 tgttgaaatt gggaatagaa cttgccaaat ggaagttgag tgatgtaggc tcaagtttgg
     6721 aaggtccaag agacgaccca tcggaacttg ggcaaagaac gtaagaacaa acgagtaatt
     6781 aaggagcaca cattatggca cgtgcagttg gtatcgatct gggaaccacg aattcctgca
     6841 tcgcaactct tgaaggtggc gagcccaccg ttatcgtcaa cgctgaaggc gcgcgcacca
     6901 cgccgtctgt ggtggccttc tccaagtccg gcgagatcct cgtcggcgaa gtcgccaagc
     6961 gtnnnnnnnn nnnnnngaga tttccgcaca gattctgatg aagctgaaga gggacgctga
     7021 ggcctacctt ggcgagcccg tcaccgacgc cgtcatcacc tgcccggcat acttcaacga
     7081 tgcccagcgt caggccacca aggacgccgg caagatcgcc ggcctgaacg tcctgcgtat
     7141 catcaacgag ccgaccgcag ccgctctggc ctacggcctt gagaagggca aggaagacga
     7201 gcgcatcctg gtcttcgacc tcggcggcgg caccttcgat gtctccctgc tggagattgg
     7261 caaggatgat gacggcttct ccaccattca ggttcaggcc accaacggcg acaaccacct
     7321 cggtggcgat gactgggatc agaagatcat cgattggctg gtttccgaag tcaagaacaa
     7381 gtacggcgtt gacctgtcca aggacaagat tgcgctgcag cgtctgaagg aagccgctga
     7441 gcaggcgaag aaggagctct cctcctccac ctccaccagc atctccatgc agtacctggc
     7501 catgactcct gacggcaccc cggtgcacct ggatgagact ctgacccgtg cgcacttcga
     7561 ggaaatgacc tccgacctgc tgggtcgctg ccgcacgccg ttcaacaacg tgctgcacga
     7621 cgccggcatc tccgtgagcg acatcgacca cgtggtgctc gtcggtggtt ccactcgtat
     7681 gcccgccgtc aaggaactcg tcaaggagct caccggtggt aaggaagcga accagtccgt
     7741 gaacccggat gaggttgtgg ctgtcggtgc cgccgtgcag tccggcgtcc tcaagggcga
     7801 ccgtaaggac gtcctgctta tcga
//
LOCUS       DAWBMW010000068         7723 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_2211_length_7723_cov_2.555686, whole genome shotgun sequence.
ACCESSION   DAWBMW010000068 DAWBMW010000000
VERSION     DAWBMW010000068.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 7723)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7723)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7723
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2211_length_7723_cov_2.555686"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    806..815
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7030..7039
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1558 a         2585 c         2200 g         1360 t
ORIGIN      
        1 atacaacggt cacctgctgc tcatcgactg cggcgtgctc ttccccgagg aggagcagcc
       61 gggcgtggat ctcatcctgc ccgatttcag ctacatcaag aaccgtctcg acaaggtcga
      121 cgccctggtg ctcacccacg gccacgagga ccacatcggc ggcgtgccct acctgctcaa
      181 gctgcgcccg gacatcccgc tgatcggctc caagctgacc ctcgccttcg tggacgccaa
      241 gtgcaaggaa caccaccaga accccaccaa ggttgaggtt gccggccgtg agaagctcaa
      301 ggtgggtccc ttcgacctcg aattcgtcaa cgtcacccac tccatcccgg acgccctggc
      361 cgtctatgtc aagaccccgg ccggctccct gatcgacacg ggcgatatca agctcgacca
      421 gctgccgctc gaccaccgca tcaccgacct cgtcgaattc ggcaagatcg gcgaaaaggg
      481 cgtggacctg ctgatgatgg actccaccaa cgccgaggtg cccggcttcg tcaagcccga
      541 gacctccatc ggcccggccc ttgaccaagc gttcgcccag gccacccgca aaatcatcgt
      601 cgccagcttc tcctcccacg tgcaccgtgt gcagcaggtg gtggacgccg cccacaagta
      661 cggccgcaag gtcgtgttcg tcggccgttc gatggtgcgc aacatgtcca tcgccgccga
      721 cctcggctac ctgcacatcc ccgaaggcac cgtcgtggac ctgaagcagg ccaacgacat
      781 tcaggacgac aagctcgtct tcatgnnnnn nnnnnatcgc cgacggcaac caccgcgata
      841 tccacatcaa cgagttcgac accgtgattc tcgccagctc cctgattcca ggcaatgagc
      901 acggcgtcta caaggtgatc aacaagctcg tgcagctcgg cgccaaggtc gtcaaccgcg
      961 acaacgccgc cgtgcacgtg tccggccact gcaacgaggg cgagctgctg tacatgtaca
     1021 acatcgtcaa gcccaagtgc gccatgccga tccacggcga aaaccgccat ctggtggcca
     1081 acggcctgat cgccgtcaag accggcgtgg acccgcagaa cgtggttttg gccgaagacg
     1141 gcgatgtggt cgacctgtac cacggcaggg ccgcggtcgt cggctccgtg ccgtgtggtt
     1201 acgtgtacgt cgatggcgat tccgtcggcg agctgaccga tgacgagctg gaaaagcgcc
     1261 gcattctcgg caccgagggc ttcgtctccg cgttcgtggt ggtcgacacc gacaatcacg
     1321 aggtcgtcac cggcccgaag atctacctga atgccgtggc cgaggacgaa agcgagttcg
     1381 acaaggttcg tcaccagatc gtcgaacagc tcaacgacca gatgatgcag ggcacgcgcg
     1441 acacctacaa gctgcagcag accatgcgcc gcacgctcgg cagctgggtt gcgcgccagc
     1501 tgcaccgcaa gccgatgatc gtgcccgtcg tggccgacat cgcccaggac gtcgaaaccg
     1561 tagaaaccaa gtagtccgga ttatccaggg acgaccgtaa ggagtaattg aagtcatgcc
     1621 cggttctaat ctcactcgta ttgaagccga agaacgtaag gccgttatcg aagcgccgat
     1681 tcactatcac gtcgaccttg acctgacggt cggcgcgaag aacttcggtt ccaagtcgtt
     1741 catctgcttc aacgcgaagc ctggttcgtc gaccttcctc gacctgatcg ccgacgaagt
     1801 gacctccatc gacctcaacg gcatgtccct ggacccggcc gtggccttcc aagacaaccg
     1861 catcgaactg accgacctca aggagaagaa cgaggtcacc gtcgaagcca ggtgccgtta
     1921 ctccaacact ggcgagggcc tgcaccgctc ggtcgacccg tccgatggca acatctacct
     1981 gtactcccag ttcgaggtgc cggacgctcg ccgcgtctac gccgtgttcg accagcctga
     2041 cctgaaggcc accttcgact tcaaggtgct cgctcccgcc agctggatcg tcacctccaa
     2101 catgccggtt gccaccatcg aggacgatcc gcgcgaaacc ctcgacggca ccctcggcga
     2161 caagccgaac gaaagcaccc gtctgtggga cttcgagccg accccggtga tgagctccta
     2221 cctgaccgcc atctgcgccg gcccgtacgc cgaatggcac accgaatacc tgaacgaaga
     2281 cggccgtacc gtgccaatgg cccagtactg ccgacagtcg ttggccaagg ccttcgcgaa
     2341 ggacgtcgac tacctgttcg acatcaccaa gaagggcttc gcgttctacg ccaagacctg
     2401 gggcgtgccc tacccgtacg ccaagttcga ccagatctac gtgcccgaat acaacgcggg
     2461 cgccatggag aacatcggca tggtcaccat ccgcgactcc tacgtgttcg aatccaaggt
     2521 gaccgacgcg ctggccgagc gccgcgtggt gaccgtgctg cacgagcttg cgcacatgtg
     2581 gttcggcgac tatgtgacca tgaagtggtg gaacgacctg tggctcaacg agtccttcgc
     2641 tgaattcacc tccacgctgg ccactgccga agccaccgaa tggcatgacg cttgggccac
     2701 tttctgctcc ggcgagaaga gctgggcgct gcgccaggat cagctgccca ccacgcaccc
     2761 gatcgtcgcg ccgatcaacg acctcaacga cacttacgtc aactttgacg gcatcaccta
     2821 cgccaagggc gcctccgtgc tgaagcagct ggcattctac gtgggccgca cgcagttctt
     2881 cgaaggtatc cacaactacc tgaaccgcca cgcctactcc aacgccaccc tggccgacct
     2941 gctgagcgaa ctggagaaga cctccggccg cgatctcaag gcctggagcg ccaagtggct
     3001 ggaggaatcc ggcatcaaca cgatcgccac cggcctgacc gtaaacgcgg acggcaccat
     3061 cgccgaactg aagctcaccc agagcgcgcc ggccgaacac ccggtgctgc gtccgcatcg
     3121 cctggccgtc ggcttctaca acgaagacgc cgaaaccggc aagatcgtgc gcaccgagca
     3181 gttcgaactc gacgtggatg gcgaaaccac catcgtcgag gccgcggcag gcaagccgcg
     3241 cccggcattc gtgctggtca acgacgatga cctgacctac accaagattc gtttcgacgc
     3301 cgaatcccag gcattcgccg aagccaacct gcatcgtttc gatgatgccc tggcccgcgc
     3361 cgtgacctgg ctcgccttct gggatatgac ccgtgacggc gaattcccgg ccgaacgctt
     3421 cgtggacatg accctgcgtc ttctggccac ggaaaccgaa tccaccacct tccgttacgc
     3481 gctggcctgc atgagcacca ccgcccacca ctacgtggcg ccagcccgcc gcgacgatgt
     3541 gctgcgccac gtggccgccg agctgtggac cctggccaac gccgccgaag ccggctcgga
     3601 cacccagttc cagctcgcca ccgcctacct gggctatggc gaggagggcg atgccgcatt
     3661 cgccgccaac gcacacggtc tgctggacgg caccgtgcgt cttgaaggcc tcgaaatcga
     3721 caacaacttc acgtggacca tcgtccaagc gctcacctcc gtcaacgaga tgaccaacga
     3781 cgacgtggac gcgcagctcg ccaagaagga gaccacggaa aaccgcgagt tcgcctacgg
     3841 cgcccgtgcc tccatggcca ccgctgaggc caaggaatgg gcttgggatc aggcgctcca
     3901 caacgacgag ctgaccaact cccagctcga ggccgtggcc cgtggcttct ccgccacccc
     3961 gcgctccgat ctggccgagc cgttcgccgc caagtacttc gagactgtgg actgggtctg
     4021 ggccaacaag acctaccaca tggccgacgc cctgctcaac ggcctgtacc cgggctacgc
     4081 cgatcccgcc acgctggtca agctcggtga cgcctggctg gacgagcaca ttgccgccga
     4141 caatgccctg aagcgtctga tcgtcgaaaa cgtggcatcc tcccaccgca ccctcaaggt
     4201 tcgcgaatac aacgaagcgc tctgatttcc ttctggctcc cctcgggcgc gaggggagct
     4261 gtcgcctcag gcgactgagg ggagtcgctt ccgcgagaag ctacgtccac attccaacat
     4321 aaaacccgca accaatcctc aacagcgtat tctcgctcaa ggccggcaat atgccggtcg
     4381 atgagcgaca aggatacggg gaatgattgc gggcttttcc ataacttcat tactgctgat
     4441 tctgttggcc ggaatcggcg caggattcgt gggctatgcg gtaggcgcat cgtcactggt
     4501 ctcatacccg gccctgctcg cgttcggcat tccgcctgta ttggcaaacg cttctaacac
     4561 cgttggcgtg gtcggcacgg gcatcggtgg cgtgatgggt gcgcgcaaag aactcaaagg
     4621 ccaagcggta cgctccatta cgtatgtggt catcggcgca ttcggcgggg tggccggtgc
     4681 gtttctgttg ctcaaactcg acccgagtgt ctttgaattt gccgcaccag tgctgatttt
     4741 gctgagctcg ctgatcatcg ccatcaatcc gcgcggccgc atgcaggcca agcaagccgc
     4801 cgcggatgcc accgcgcaac tcaagcacat cgaagccact gaagccactg aagccgccaa
     4861 gcgcgcaacc acccctaccg cacctcaccg cccgccggaa caactcgtgc agcccatgag
     4921 tcaggattcc tggtgggtat ggctgggcgt agcggcggtc gccatctact ccggctactt
     4981 cggcgcgggc gccggtacct tggctctcgc cgtgcttgat gccgccaaaa tcggcccatt
     5041 ccacaagatc aatgcattga aaaccctgat cggcacaggc gcgaacatca gcgcctcagt
     5101 ggtattcatc atccaaggcg cggtcgattg gccggccgcc atcatgctgt gcatcggatg
     5161 cttcatcggc gggtacatcg ccccgtccat cacccgcaag attcccgcaa acatcatgcg
     5221 cgccgccgcc gtaatcgccg gcatcattct taccatcgat ctggggctca aaacctattg
     5281 attcgtgcct tacactgatg cagtgaagca tacagcatgg ccgtcaaccg cagcgtggtc
     5341 gcagaccccg ataaggtggc caatgtctgg catttggcga ctgcggaacc tgagcgccta
     5401 gaaaccagcg cggcaggcga agcggtgaac gacttcctcg atccgccaga cacgtcgcag
     5461 attcacggca ttgcgtgtca tgctgattgg cggttcgcgg cgcgagcagg tgctgttcgc
     5521 cggcgttatg aaggagctac tcgcgcccat caacaatccg cgatatgtca tcatcggtaa
     5581 ggaatggggc gtgcgcgctt actgcgtgag cttcccctgc ccgtccgtat ttgcccgccg
     5641 ccagcaggac gccgagatac tgagccggca gttggaccgc tgcctgacgc attgcaccat
     5701 ggtatacgcc cgcactgagg aaggtcggca tacgctactt cgctgccaaa cgcgttcttt
     5761 cctcaaccgc gacgagcaac tgccgcacat actgaccaca acatcggagt aatagatcgt
     5821 tgcattcatg gccgagacca ccccggtaga ggagatgccg ttgggcatgt tcatcgcatg
     5881 gctcagcgtg ttcctcgccg tcgttctgtt tggtgttgct ttgccggtta tcgatgtacg
     5941 ctgggtagtt ccgttcgtcg tatttgccgc tgtattgttt acaggcgcgt ttgccggccc
     6001 gttcttacta gtgcgatggc ttcgttcccg tgccggagtg catcggtccg cccggcatgc
     6061 ccgctaacga atgtatctca tcattcatcc gccgaacaag atgaatatgc gggtttgtgg
     6121 gtgatgccat tgcggctgtt gaatgctgat ttccggttct ggagcagatt ccgtggcggt
     6181 tttttgccga gtcgggtcct agggtggagc acaacgacta cgatggtgcg ggtaacgcga
     6241 tacaaggagt ggaacttcat atgccgaaga tgtttggaac cgatggtgtt cgaggactgg
     6301 ccaataggga tttgaccgca cgtctggcgt tggatttggg cgatgccgcc gtgcgtgtgc
     6361 tcggcgacgc cggcacccaa gacgatcagc ctgaggggcg ccgccgcgcg ctcgtgggtc
     6421 gtgacacccg tgtgtccggc gattttctgg cttccgccct gtctgcgggc atggccgccg
     6481 gcggattcga cgtgatcgac gcaggcatca tcccgacccc gggcatcgcg ttcctgacca
     6541 gcgtgctcaa cgttgagatg ggtgccgtga tctccgcctc ccacaacccg atgcccgaca
     6601 acggcatcaa gttcttcgca cgcggcggct tcaagctgcc cgaccagaag gaagacgaca
     6661 tcgaggccgt gctcggccag gactgggacc gcccgaccgg tgccggcgtg ggccgcgtga
     6721 gccacgacca gaccactgcc accaacctgt acatcgatca tctggtggcc accatcgctc
     6781 cgctgaacga tgacaaaacc cagcccaagc cgctcaaggg actgaagatc gtggccgact
     6841 gcgccaacgg cgccacttcc gtggtggctc ccgaagccct gcgccgtgcc ggtgcggatg
     6901 taatcgtgat caacgcctcg ccggacggct acaacatcaa caagaacgcc ggttccaccc
     6961 accccgagca gctgcaggcc atggtcaagg ccaccgatgc cgtgatgggc gtggccttcg
     7021 acggcgacgn nnnnnnnnnc acgacaccct cgtggtcacc gtgatgagca acctcggtct
     7081 gaagctcgcc ctgaaagata tgggcatcaa gaccgtggaa accgccgtgg gcgatcgtta
     7141 cgtgcttgag gaaatgctca agggcgatta ctcgctcggc ggcgagcagt ccggccacgt
     7201 gatcaaccgc gaattcgcca ccaccggcga cggtaccctg accgcactga ccctgtgcaa
     7261 cgaggtcgtc aagtccggca agagcctgaa ggagctcgcc gccgacttcc cgcagctgcc
     7321 gcagaccctc atcaacgtgc cgaacgtgga caagaaggcc gcctccacca acaagcgcat
     7381 ccaggatgcc gtggcccgtg aggaggaact gctgggagac accggccgtg tgctgctgcg
     7441 cccctccggc accgaaccgc tcgtgcgcgt gatggccgag gccgccaccc aagcctacgc
     7501 cgacgaagtc tgcacccgcc tcgccaagat cgtcgccgaa gaactcgcgc tgtagcaccc
     7561 ttccctaagg aaaaaacaat gtttggaaag aacgccaaag tcgacctcga actcaaccgt
     7621 gatgtcgaac aactcatcaa aaccggcggc aaggagaagc tcctgcccat cgtgcaggcc
     7681 ggcgaaccgg tgttgcgcca acggacagtg gcctaccatg gcc
//
LOCUS       DAWBMW010000069         7577 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_2257_length_7577_cov_2.099575, whole genome shotgun sequence.
ACCESSION   DAWBMW010000069 DAWBMW010000000
VERSION     DAWBMW010000069.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 7577)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7577)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7577
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2257_length_7577_cov_2.099575"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    688..697
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1663..1672
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2181..2190
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1454 a         2228 c         2343 g         1522 t
ORIGIN      
        1 gatttgaacc cgcgacccct tgacccccag tcaagtgcgc taccaaactg cgctacggcc
       61 cgatggtgca aaagcaccga gtagataatt taccagatat ttgaaatctg gcaaattacg
      121 cgcgtgtcac ttccgcttct tcttctcgcg gatgttcacg gagatctgaa tcggcgtgcc
      181 ttcgaagccg aattcctcgc gcagggaacg ctcgatgtag cggcggtagc cgtgctcgag
      241 gaagccggtg gcgaagatca cgaaacgcgg cgggcgcgtg gatgcctgcg tggcgaagag
      301 gattcgcggc tgcttgccac cacgcaacgg gtgcgggtgg gcggcctgaa ttttgccgag
      361 gaaggcgttg agcttgcctg taggaatacg ctgatcccag gattccaagg ccttatccat
      421 ggctcgcgtc agacggttgg tgtgccagcc ggtcttggcg gacagattca cacgctcggc
      481 ccacatcaca cggtcgaatt cggtgttcca caggcgttcg agacgctgct tgtcgaactc
      541 gtccatcgca tcccacttat tgaacacgag cacgatcgca cggcccgcat ccacggctgt
      601 ggacatgacc ttcaaatcct gatcggatac cggctgcgaa gcatcaaaca ggatcagcgc
      661 caattcacag cgctcgatgg cggcctgnnn nnnnnnnttc cgcgcctgtg agcttgtgct
      721 ggcgtcgctt gatgccggcc gtgtcgatga acagccagtc ctcgccatcg atgttgacga
      781 tttcgtcgac ggggtcgcgc gtagtgccgg ccaaatcgtt gaccacggca cgttcttcgc
      841 gcgccagctg attgagcaga gacgacttgc ccacgttcgg gcggcccacc agagccacgc
      901 ggcgcaggcc ggaaggcgtg aggtagccgg aagtcttctc ggcctgcttg agcttgtcga
      961 gcgcgacgtc cagcaggtcg cccactcccc tgccgtgcat ggcggaaatc gagtatggtt
     1021 cgccgaggcc gagcttccag aattcggcgg caagatattc gctggcctgg tcgtcgatct
     1081 tgttgacggc cagcacgacg ggcttgccgg cggcacgcag catcttgacg atgcgctcat
     1141 cggtggtggt catgcccact tgaccatcga caacgaatac cacagcatcg gccaaggtca
     1201 cggcaacctg tgcctgtgag gcgatggcgg attcaatgcc ttccacgtcg gcttcccagc
     1261 cgcccgtgtc caccagcttg aagtcagtgc cggcccattc cgcgtcatag ctcacacggt
     1321 ctcgcgtaac gcccggcgtg tcttccacaa cggcggcacg gcggccgagg atacggttga
     1381 ccagggatga tttgcccacg ttcggacggc ctacaacagc cagcacaccc accggcttgg
     1441 ggccggcctt gcgcgcgcct tccgtgaaac cgcggccgga aatcagatcc tcatcgcctt
     1501 cgtccagctc atagccttcg agattggcgg catactgctc gtactcctgg ttctcgatgg
     1561 cgtcctcgac cagaccgatg agcagatcga gcgtgtgggc gaagtccaaa tcggaattgt
     1621 cgatggtagt aacgccatcg gcggcggtca ggaagctggt gannnnnnnn nnatcggcct
     1681 tgtcgcgcgc agccacatcc tcagcaccca cgcccttgac ggcttggccg gtgcgacggg
     1741 cctgacggac ctcctcacgg gcggtcagca gcacgcgcac ctcggcgtcc ggggacacca
     1801 cggtggtgat atcgcggccc tcggccacga tgcccaaacc cccggagaag gagtcggccg
     1861 acgcttcgcg ggcgatgtag gcgcgctggg cggcgatgag cacgttgcgc accgggatga
     1921 cgttcgacac cttggagaca tggctggaga cttcggagga acgaatggcc tcggagatgt
     1981 cctcgtcgtc ggcgaatacg cgggggttgt ccgggtccac ggagatatcg aaatggtcgc
     2041 cggtgaagaa ctcgcccacc gcttcggtga tgacacgctc atccacggtt tcggcgtcca
     2101 gatcgatgtc ctgcttcaga caccaccaag cgcaggcacg atacatcgcg cccgtgtcaa
     2161 ggtaagcgta tccgaaatac nnnnnnnnnn gtcgatggca acgcgaatca cagaccaacc
     2221 tccttggaca gcgaacggac ttccgtctgg gacagcacgc ggtaggaacc gggcttcaaa
     2281 tcaccgagct tgatcgggcc gatctgggtg cgcaccaagc gcttgaccgg gaagccgatt
     2341 gagccgaaga tacgtcgcac gatgcggttc ttgccggagt gcagcacgac cttgaccatc
     2401 gtgtggtcac gcgaggagtc gatgatcgcg cagtggtcga gcttgatcca gccgtcgtcg
     2461 agctgcacgc cggtggtcac gagtcgacgg cacacggtgc cggagatctt gccttccagc
     2521 gtagcgacgt aggtcttctc gacctcgtac ttggggtgca tgacgtgctg cgagagctcg
     2581 ccgtcgttcg tcatgaggat caggccttcg gaatcgtagt cgagacggcc catgtggaag
     2641 atgcgctcgt acttgtcgcc cacgatgtcg cgcagcgtgt agcggccctt cgggtcgtcc
     2701 atggcggaga gcaccttgcg gggcttgttc agcgccagag tgacgtggtt cggattgacg
     2761 cgtacgcgcg aaccgtctac gcgcacctgc tgcttatgag ggtcaacgcg agttccgagc
     2821 tcggtgacca gttcaccgtc gacctcgaca cggccttcgg tgatcatgtc ctcgcacttg
     2881 cgacgggaac cgaatcccgc ttgggcaagc agcttctgga gtcgaatgga attggaatcg
     2941 tcttgcgctt tcgcagcgcg ggaatatgcg tttggcatcg ttctcccaac gtggccggac
     3001 agattctgtc atcgcagaga ctgtcgattt gtatacatac ttcagagttg gcggactgga
     3061 atcccagcca atcccaacct atatatcata ccgcaacgta ggactgctgc tagactggca
     3121 accgtttgtg cgggttcgcc cgtcttccga cagatgaaaa ggaatgaaca atcatggaag
     3181 aaaccaagca acagccgctg gctctgcgcg tgtgcgccga attggccggc agcttcctgc
     3241 tgtgctttgc catttacgcc ttctccacat ggggttcggc cgtgtatggc atcaatatcg
     3301 tgttcttcgc gttggctacc ggcttggcat atgccgcagt caccgccatc ttctcccgcg
     3361 tgtccggtgg ccagctgaat ccggccatca ccgtagcctc tgtgctggtt tccaagacga
     3421 aaatcctgga tggcatcctc tatgtgatcg ctcaggtcat cggtgccatc gccgccggct
     3481 tcgccgtggt caagctgctg cccacatccg agcaggtagc tgccaaagtg tggctgactc
     3541 cggccgttaa cggtttcgaa aacggctccg tgtcttactc gctgctgacc cagtatggca
     3601 ttacgttctc gattacgctg gcgattgtcg tcgaggtcgt tgcctctctg attgtcgttg
     3661 ccaccgccat gtcttctctg ggtgatcacg gcgagtcctc ggaccgtcat gtcattgcca
     3721 tgggtcttgc ctatggtctg gccaccgcca tcagctaccc ggttaccggt gccggtctca
     3781 atcccgcccg ttccaccggc atcgcactgg ccgcgatgaa tgagggtctt acgcaaaatc
     3841 cgctgcagca actgtgggta ttctggatta gcccggtctt ggccgccgct gtggtggcgt
     3901 tggtcatgat catcgcgcag atgatgacca ctcccaaagt gccggctgcc gccgatcttc
     3961 cgcagacgga tgccgatgca aacgcggaca cgctcgacga tggcgaattc ttcgatcagg
     4021 ccgcctcgtc tgatggcgat tcagcggaaa aagatgttgc cgaagagtcc aaagaagctg
     4081 aagtaggtaa tcagcaggcc gattcccaag gcgatgccga tgaaggagtc gaacgccact
     4141 gagcgcactt tccatgggct tctgtcctga agaatcaggc gaacaagccc ggtgatgatg
     4201 gccgtcacgg caagtatgac cgtggcggcc atcgtatatc cgaaagcaac cagcagtgtg
     4261 atgacggcga cgacgccgag tacgcaccat tcaaaccatg gcttgccctc atgtgcttcg
     4321 gatacatagg gatggttgcg catgttgcga ttccttctgt cgaacggtta cgtgaccaca
     4381 atagcaggcg aatagcaaag gtgggattcc gcttggaccc gttgacgggt caggcggaat
     4441 cccacctttg tgcgttttgg ctgagctgct cccctcagag agtcccgatc agtggaagaa
     4501 gtggcgggtg ccggtgaggt acatggtcac gccggccttc ttggccgctt cgatgacctc
     4561 ctcatcgcgg atggagccgc caggctgcac gatggccttg acgcccgcgt cgataagcac
     4621 ctgggcacca tcggcgaacg ggaagaacgc atcggatgcc gcgaccgcac cggtggcgcg
     4681 atcggcacca tcggccagcg tgttggcgcg ctcgacggcc aaatggcagg agtccacgcg
     4741 gttgacttgg cccatgccga tgccaaccgt ggcctggtcg tgggccagca ggatggcgtt
     4801 ggacttcacg cagcggattg cacgccatgc gaacacgagg tccttcagcg tgtcagcgtc
     4861 ggcggcctcg ccggccacga gcttccaggc gtccggatcg tcgccgacgg cgttgatcag
     4921 atcagtgtcc tgcacaagca ggccgccatc gatctggcgg atggccccgt ggcccttcgg
     4981 cggctcggcc acctcgagga tgcgcaggtt cttcttcttg gtctgcagca gttcgagcgc
     5041 ggccggctca tagtcggggg cgacgatgac ttcggtgaaa atcgggcgca cgctttcggc
     5101 catctcaaga gtgaccttgg agttgcaggc gatcacaccg ccgtaggcgc tcatcgggtc
     5161 gcaggcgtga gccttcttgt gggcctcggc cgcggtcgcg ccgatggcca gaccgcacgg
     5221 attgttgtgc ttgactacgg ccacggcaat ggcgggagcc atatcccata cggtgcgcca
     5281 ggcggcgtcc gcgtccacgt agttgttgta gctcatcggc ttgccgccga gctgctcggc
     5341 gtgggcgaaa ccggtctggt tgagcgggtc gatgtagagg gcggcctgct ggtgggagtt
     5401 ctcgccgtag cgcagggtgt gcgcacgatc ccaggtgcgg gtgaactggg ccgggaactt
     5461 ggcggaatcg acttcagtgc cctgatcgtc cttgtccacc tcgacggcgt ccagagaggc
     5521 cggcttgggc cagtgcttgg cagtccactc gttgatggtg gcgtcatagg cggcggtgtg
     5581 cgcgaaggcc ttggcggcaa gccacttgcg ctcgtccaag gagaagccgg tgccgtcggc
     5641 cacgcgggag gcgaccagtg cataatcggc cgggtcggtg acgatggcca cggtggcgtg
     5701 gttcttggcc gcgccgcgca ccatggaggg accgccgatg tcgatcttct cgatggtatc
     5761 ggcctcgttg gcgccggagc gcacggtgtc ggcgaacggg tacaggttga cgaccaccag
     5821 atcgaacggc ttgatgccga actcctcgag ctgcttggcg tgctcggggt tggtcatatc
     5881 agccagaatg ccggcgtgaa tgtacgggtg cagcgtcttg acgcggccgt cgaggcattc
     5941 ggggaatccg gtgacatcgg acacctcggt taccttgacg cccagttcgg caagcttctt
     6001 ggcggtcgaa ccagtggaaa caacttcggt gccggccttc acgaacgctt ccgcgagtac
     6061 ttcgatgcct tccttatgaa agaccgagac cagtgctcga cgtatcggtc ggttcgtgtt
     6121 tgtcatcctg ttcctccttg gattcttgtg gttgtgacgc taggaaccgc gcctgatgaa
     6181 cggtggcggt cttcgaggtc gtggcggtgg tgcgggaatc cttgattctc tcaaccaacc
     6241 agactatcgg aaaaacgagc gctatgccaa tcagcgcgag cagccatgcc gcggtaagtc
     6301 ccagtgcggt cgaatgaccg acgactctgg tggaggccat gacgtcgacg cctatgtgag
     6361 caagacgatg atcgccaagc gatccattgg acagagcgaa cagcaatgtg gatgccagtg
     6421 agatcagcat cgcgctcagg cagaatgccc cggcggagta gatcagcgaa accagtaccg
     6481 ggccgcgggt ggacgtattc cgtacattca acggtctgca ggcgaatcct tgcggtagga
     6541 atacagccag caggcccacg agcgcagcga tggcgagagg cgcgttcaag gcaacggtac
     6601 gccatagttc actgctgacc ggttcgggca gaataccgaa taccggaatt gcaggaagct
     6661 ctttggattg gccgagccat aatgtgaaac tggccaaatc accgatggcg aatccgccgc
     6721 cgaagagcca ggaaacagcc cagagcatga cgttgggtag ccagatcagc atggcgacgg
     6781 tggtgagaat tcgtgaaccg gtttccatcc cggacaattc gaacaacgat acgacagcgg
     6841 catggttgcg taccgaccat acgatgacgg taatcaatcc aattgccaga tatatgccca
     6901 gtatcgcgac ggccaaggcg acgccgattt tcaggcagtg gcgtatgtcc gccggcacct
     6961 gcttgcgagt ccagtccctg aatgctcgca ttttggctga ttccggtacg gctgcgcata
     7021 ggaaaccgat cgagaatacg atggctgctt tcagcaacac cagccactgg tcatcgacaa
     7081 gacctaggtg cacggattgc cggaatgcct cgttcaaccc gagccaaaca atcaatccgg
     7141 caacatacgc atgcggcccg tatgctttga ttcgcatgat gagtgcattg atcagccata
     7201 tcagcagtat ggtcaacagc aatggcgtaa tggtgagggt gaacgaatcg gttctaaagc
     7261 ccgatccctc actcagaagc acaacggcct gtgtcagcga ggttgtgccg acggtgagat
     7321 tgtcgccgcc ctcttccata gagacgacca ataacatcag cgctgtatag cagcctaatg
     7381 caacggcata gatggccatg gatgcgaggg cgactatggc tccccgcacc cattggcgaa
     7441 tcacggcttt catcacgatt ggttggaacc gatcagtcga tgttggcctt ggcgagtacg
     7501 tcacgcatga tttcggcggc ctggcccggg gtcttgccca cggggatgcc cgccgcctcg
     7561 agcgcgttct tcttgtc
//
LOCUS       DAWBMW010000070         7482 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_2292_length_7482_cov_2.419820, whole genome shotgun sequence.
ACCESSION   DAWBMW010000070 DAWBMW010000000
VERSION     DAWBMW010000070.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 7482)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7482)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7482
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2292_length_7482_cov_2.419820"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    408..417
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1937..1946
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5487..5496
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1450 a         2187 c         2191 g         1624 t
ORIGIN      
        1 agccagttcg atttgggctt cgtgattatc tcggcgctgt tgcaaggtgt tttcgctgag
       61 attccgttcg ccttgacccg ttaccgtatg ttcaatctgc cgatttccat tgcatccggt
      121 gcgttggtcg ccttggaata cggcacatat ctgatgctgt ttcgctacgt gggtgtgtca
      181 ttcgtcagcc cgcgtggcat tgtgcacatg atttccgagg tcaccggcgg cgtgcttatc
      241 agcggcgtga tggcctggta tctgatgctg gccatcgcca aaaccggtgt gctcgaccgc
      301 ctcgcatccg gccgcgcact catcgagcga cgcgcctgac actctttgcg cgccactttc
      361 ccgggaatgt tgcgcggatt tggcggctgg gtcgatctca cgaatcgnnn nnnnnnntag
      421 agggggtttc gggcatgcta gcgcaacaat cccgggaaag tggcgctagc cgcgtctctg
      481 cggtgattgc attgtggggt agccgattga tgctaagcat tgacaagttg atgtgcccgt
      541 tgccgtgaga ccccgagtat atcgcctatt tgctgtaggg cgagacccat gccatgtagt
      601 tcttgtgctg cggcgcgagt ttcccgcgct gcgttcgatc tgcattccgc ttcttccttg
      661 cgcagtcgcc gagcttcatc aagatgctcc tgcacggatt gcggcagctt tgtctctacg
      721 gtgagtgcga aatcagcatc ttgtgtcatg acggtgataa ggtcgcgtgc catactttca
      781 atctctgctg ttgttcttgc ctgcgtgaaa ccgataccgg gaatctcaag aatccagtat
      841 ttttcgccag gacgaacgat gacctgatat gttttcactg ctgataccag cctttcccga
      901 gtttggcttc tgcttctttg caaataatgt ccgcgaggtt gttgtcgatt tcgttatgac
      961 gtgggatggg gaatcataag tccatcgagt tggaatatct catgactccc tccttgcctg
     1021 atactttcaa acgtgacatt cttgcgttct gccgcttgct tcatcagttt gatgagccat
     1081 ctcctactcg tcattatatt gttaactcct ttcttgacaa ctctgtcaag tgtggtattt
     1141 acttttaacc gccgaacgtg ctgaactcgg tggttcgctc gctaggggca atggtcgcgg
     1201 actgggtcat gcggccttgg tagtagcggg tttccttgag ctctccgttg ataacttcct
     1261 ggaaatgggc ttcgtggcgt tgcacggcgg gggagctgag gtcggtggcg ttctgatagg
     1321 cggcctgatg gtatttgatc cagtgcgaca actccctgcc gttggttgaa ccagccacgg
     1381 gtgcggcgtc atatggggtc agaccggtga tgggcgttgc gccgggagtg catgagccgt
     1441 ccgaatgcac tgtggatggt gttgccgcgg gcgcgggcga tacatgccct tgtacggtga
     1501 gccagttatc ggtgaccggg ttggcggcac catccagtgc ggcgaccagt tcatcatcga
     1561 tttccacgct ggtgacccac gtgcgttgcg gaatcggatg attggccacc ggcgatccgg
     1621 ctgtcaccac atgctcgatg gtatattcct ccgcccaatc cgaggcaagc gtggcggcaa
     1681 cgataccacc ctgggaatgg ccgatcagcg ccacgggctc atccttgccg atgcctgcct
     1741 gacgcatcgc ctcggcaacc atgcgagcgc tgtccgcttt cctgcggcgc tcctgatcgg
     1801 cgctcatcag ttccacgttc tgcgcccagc caaatggtga gtcaggctgt ccatcggtac
     1861 ccgggatgtt gaccagccag gagttggttc catcagatcg ttcatagcgt tgaatggcga
     1921 tggtcccata ttcgagnnnn nnnnnngttc agctcgattt tgccgagccg ctcttcggcg
     1981 agcctgcgca gattctccat tgactcggcc accgatccgc ttgcgcggac aacgtcggca
     2041 ttcgcctgca cctcgcgcac gtccagatga ttgccttgga cgacgtcttt cgccgggccg
     2101 gagaaattgg cgattttccc cgcagccttg ttgacctcat cggtgtgcgc aatgctcttg
     2161 ccgataggta cgccgccaat gattccgccc gcgccgctaa gcactccttc ctggaacgga
     2221 taggtgaacg tggacatcca tgccgggttc ggtttgccgt caatagtcca gccggccaga
     2281 aagccgcccg ccgccacagc ggcaacaccc gccgcggcat atcccggttt ggcctgtgtg
     2341 ccggcctgca ataattcggt aaacatgcgc cgagccgtca tctccgcctg agaatacaga
     2401 gagtgtgcgc gaatcagcag atcggccgcg ccacgcagat catcgctcaa ccgctgacat
     2461 gccgttgcat ggtcataaca ccgattgccc agcgcggcat atggtgcagt gacatggccg
     2521 ggaacaacgg cgctcggatc gcccgactgc aaggtgacgc acatcggcgc actatgccga
     2581 tgtgactgca actgtaatgc ggccgattcc caagccgtgg ctatggccgc aaatcctgcc
     2641 gcctcgtcat cgagagcctt ggctatggcg gcatattctt caagagtggc tggcgcatat
     2701 cccactccgc cgtaaacgga cgattccact tgccagtaat cgtgctcgct catgacgacc
     2761 cccaagccaa tcggtgggtg gtctccatgt cttgcgcgag actggccgaa tactgtgcgg
     2821 tgcgactgag ccgcgatcgg aatagatcgg cggctttacc ggtccacacg atgttgctgg
     2881 agcggttcac ggccaccgcc gtctggtccg attgcgccag tgtggccgca cgctggacgg
     2941 acacgaggtc gcgactacgc tgtaccgccg catggcaagg gcaagtgccg taatcgaacg
     3001 gcgtcaccgt attgccggca aagccgccgg tgggatcgat gggtgctggg ttgctggtgt
     3061 aggtattcat atatccagct aaaaggacaa gactcatgct tgtccagccg gaacgaccaa
     3121 tgtggttcta gcttgccgat tttgggtgaa gttatcccca agtgttgtgc atagtcgtaa
     3181 cgattatcca caaatagcct gtttggacat accttggatc agccatggcc tatcgaaaat
     3241 tggataaatg ccaaagccga cagccgaaac cgggaaacag ccgcgataaa gccatgaccc
     3301 gtgagggtgt gagaaaaaag aacaactgtg aacgactgct taccacggct tggtagcccc
     3361 ggtacccttg ttctgcgtcg ctttctgctc ggattgggtg gtgttcgact gggtatcggt
     3421 ggattgctga ttggccttca tcagctcctc gacctgcttc ttctgttcgt cggtcaacgc
     3481 gccgcccttt ttggcattgg atgaagaatt ctcggattgc tgtgccgact gggcttgccc
     3541 ggaatcggaa ccgctgttct gcgcctcggc ctgtttcagg gtgatctttg tcagctcgga
     3601 agaaatcgaa gcattggtgt cgatggcgtc cttgacttgc ggcaacagaa gcgtgcgcat
     3661 tcgcccggcc tcgtcgaatt gcgcgtcggt ctgctgctgt tgcgccttga gcgtggtgat
     3721 atccgtggac tcgtcctgag ccgctttgat attggcgttc aatgatgcgg tggcctgatt
     3781 gaacgtaatc gaagcggaca ggtttactgc cgccacgccg gcaaccagca acagcaatac
     3841 ggcgccacaa gccaggacga tacgtgccca aagcggacac ttcgcccgca tccgagtatt
     3901 cgccgagcca tcgttctcgt ggatttcgtt actgtttctc acagcagtct ccttgaggtt
     3961 gcgatccatg caccgacttc catggcaagc aatatcgcca acgcaatggc caacggccat
     4021 accaccggcg tggtgcgtgt gcgctccttg accgtggtgg tcatgcgcca ctgcttcgat
     4081 gacttggccg aaaccgaatc cgccaaagtc tgtgaagcgt tcacggccac atacgtgcca
     4141 ctgatctcgt cggcaatgtc tttaaggttc ttctcatcca tcttggagat gccgggttcg
     4201 ccggtatccg ggtcgaccac ccagtggtcc gtggtgttgg aatcgccggc actgacgcca
     4261 tcggcgatga cgggaatctt gccgccctcg gtcgagccga cgcccacggt aaacccatca
     4321 ttgagatact ggcgcagtga cgagaacgta cgacgcgtca cattcgaggt ctgttcgccg
     4381 tcagtgatga ggtacagcac aatcgcatca tccgggtgcg cttcacgaat cgacttcgcg
     4441 gtcagcagca attgatcgat cggagcatcc agactggagc ccgccgacac ggatgtggct
     4501 tccaccgcca acgtgttggc ccaattatcg atggccggcg catccggggt cagcggcaca
     4561 tcaagcgttc ccgaagcacc gaaccgtaac gccgcgaaac tggcgtccgg ataagcggcg
     4621 gtcacatcat gcacggcctg acgtgcggca tcgatgcggg taatggtctt ctcggacccg
     4681 tatgtggcat cggccaccgc catcgacccg gtggtatcca ccgccacgat gacgtcggtg
     4741 gcattgatgg cctgtgaggt ggtagacgat accacgctgg gagtcagtac catcaccgcg
     4801 acgatgagca gcattagcgt acgtcgtatg caagcccacg cggtctcgtc actggaagca
     4861 cggcgacgaa caaacaatac gatttcgaga atggccagta cggcgagccc ggccgccagc
     4921 agtccgccgg cgacccagcc caaggcgggg gagaaggtga atccgttcat cgcctcaacc
     4981 tccatgcaca tacgatccag ataatgagta tcacggccag agcaagcgtc cacagaccgg
     5041 gagcgtccac cattgaggat ttcgccttgt tctccacatc cgtatctcta cgtgactgaa
     5101 tatcccgtac cagctcatct atcgacgcgc cattcgattg ggtgaggaag atgcctccat
     5161 gcgactcgat cgcggacttc atatccgttg tggtttggtc cgattcactg gctttcggcc
     5221 cggaatacag gccgtccacg gtgattttgg tctgctgggt caaatccaac gcttcggtca
     5281 gcgaataggt gggcttgccc gacacgacgt tgtcggtggc cagcacgatg gatgccgcac
     5341 gctgacggtc ggcattcgca tgattggctt cgccataagc gaaaccgggg agcatcgccg
     5401 cgcaagacac cacgccgtcg ccaatcaagg aggtggcgtc cttgcggttc tgcgtgcctt
     5461 cgagccaatt ggcgatgtcc tgatatnnnn nnnnnntcgg acatcttatc gatgtcatcc
     5521 tgcgactcga cgcctttcag cgccttgctg gcggaggtca actgtttggt gaccagttcg
     5581 tagtcgtcgg tcagcgggaa caccgtgcgt gaggtcgagt tgaagatact caaaccgata
     5641 cgttccccct tgaaatgctt gaccagttcc aaataggtgt cgatgacctc gcgatcatag
     5701 gggagcgtgg accccgacac atccagacac aggacgatat ctcggctgga agcgcgttcg
     5761 tcaccggcat ccacttggct ggggcgagcc accagcacaa tggccagcgc cagcgcaagc
     5821 accagcacgc tgatggcgag acggcccaaa gcgcgccatt gacggaacag gcgggaagcg
     5881 tgctcggtat tgagatcgtc gtccatcgag aatacagggg tgtcgtcggc ggcgttacgg
     5941 cgggcgaaca cgacaatcag cacgataatc gccagcgtcg ccaagacgcc ggccagagcg
     6001 gcccaaggcc aatgccatgc cagcatcatg aacgccacct ttccaccaga ttcgccaccc
     6061 actcggcggc ctgctgcacg ctggtcgtct gggcgattcg gttgtgggca tcatcggcaa
     6121 agtccggtgg atacaacgcg ccaatggtct gcttcaatgc gtccaatcct tggcgattcg
     6181 cgggcgtgcg attcaggtac gccaaatcgg tcagcgtact ggaactaagc tccttgcctg
     6241 atgcggcacc ggcgaaatca cgggcgatga cggacaattc gacgaaagcc tcatgccggg
     6301 gcagtgctcc ggattcatga cgggccacca catcgtcgat gcgggcacgc cactcggcct
     6361 tggagctggc attgctatga gcgccacgcg cgcggtcgct gttccgaggt ttgcgtgcgg
     6421 ggcgagacag catgatgatc aacgcgatca gacctaccgc cagcaacagg catgcgacga
     6481 tggccaccac caggggagtg gtcaaggcca acgactccac gggcctgaga tcgccgggat
     6541 cagtggcgag cgggatgatg gggaacatgc tgtgcatcat agatcaccag ccaaagtcag
     6601 tccgaccagt tcgggtgcag tacccagtct attgcgtgtg gtgcgggcca atgcccggga
     6661 caccagggcg acgaaacgat cgaacatgga ttcgctggat gcggatcgaa tgatatggga
     6721 accggcacgg gtgagctcct gttccaaagc cgcggccata tacgcgcgat gagtgtcgac
     6781 ctcggccgct gccttggcgt tgcgcaggaa tgccggcaca cgccgtgcac tcaagccgtc
     6841 ggtgggagcg tggcgggatg agacggcctt gaacggattc atcgtcgcca catcgatcag
     6901 caccatcgga tgggtgcggg taatacgacg gatcgtggtg atatgccgct cctccatcgc
     6961 atgctcatcg gtggccaata cgatcaatgc ctcgcgatcc ttgatacggc gcgcatattc
     7021 cagcagcgcg tcgatattac ggtgatgatc ccaatcacgg tcaagcgcct tgtccagcgt
     7081 gcgttcgaac tgcgcgaacc caccgttgaa ccgtacgcgg gtgatgctgg actcatcgcc
     7141 gaacaccagc gaaatatcgt ccgaccggcg caacgacagt gcggcgaaca tacgcagcgc
     7201 attggcggca acctgccatg cgcgttctcc ggaagggcat acgccagtca tctccaggcc
     7261 cacgtccatc agcaaccaga cccgtgacgt cgacaggcgc tcacgctcga ccaccatggg
     7321 gcggccttgg cgggcgctgg tcttccaatc gataagacgg gattcgtcgc ctggctcata
     7381 ggcatgcaca tccatcgcat cgccgctgcc gccacgacgc ccggatgcgt gttcgccctc
     7441 cagcgcgccc aaagccttcc ttacggtagg caggctcagc tg
//
LOCUS       DAWBMW010000071         7471 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_2297_length_7471_cov_2.649811, whole genome shotgun sequence.
ACCESSION   DAWBMW010000071 DAWBMW010000000
VERSION     DAWBMW010000071.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 7471)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7471)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7471
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2297_length_7471_cov_2.649811"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1429 a         2324 c         2356 g         1362 t
ORIGIN      
        1 ctttggtacc agtaggcgac cgtggccacg tcgtcctgtc gttcgaagtt gccggcttcc
       61 tcggtgccga tctgctgcca ctcgacgcgc aggtctcgct cgaagtagat cgggtcgggg
      121 atgtgccaac ggtataggcc gcgggtcacg ggggtggcag tgtcccagta ggcgctttcg
      181 cgtccggcga ggttctgcga gtagaacggg aagccgaggt atgggccggt gaaggtgcgc
      241 tcgtgcatgc ggccgtgttc gtcgaattcg gcgaagctcc aggccgaagt agtcctcggc
      301 gccggtgctg caccaggtgg ggtatttgcc gtcgccgtcg atgtagaact tgaattcgcc
      361 ttcgccccac cagcggcttt cgagcgcggt gagcgccagg taggtgccga cgtaggtgcc
      421 gtgtccttcg acgccatcga gcaccacgta gtcgtgggcg agttcggtga cgcgttggcg
      481 gcgccactgc gcgtggaagc gcatcgtgtc ggccggcagc tggtcgtatt cggtgtagtc
      541 gatttggtag aagaacgcgg gcacgtcctc gttgtggtcg ttgcgcagca cgatgcgcgc
      601 gtgttcgaac ggcatggcga agtagcagtt gaagccccgg ttcgggttga ccatgacggg
      661 aattgagttg atggtgcagg actgggcgtg tccgcagcag aagaagtcgc cgatcgggca
      721 ttgcaccgag ggggagtcct cgccgtccca gtagaattcc aggatcaggt tgcgtagcac
      781 gtcggggccg gtgggggagg tgcggtcggt taccgtcatc cagatgtgac ggatcacgcc
      841 ggggccctcg acgtccatca aagtcaccga ttccccggac ctcaccgtgc tgatgcaggg
      901 gcttcccttg cgggaggggc ccaacgcgct ggccgccatg gccgcgcggc ccttgccgcc
      961 gtccgggttt tcggcgttga tgcagcgggt gcggccgggt gccctcatgg ccacggcatc
     1021 caaggcgctt tgtccgagcc ccggcgcggg gttcgctgtg gtgaatgcgt cgagcatgat
     1081 gcttacttcc tttcctgtcg caggcccggc ccgccacggc gttttgtctg gggcgggctg
     1141 ggctgctggg ggggtgttat ttcacggcac cggcggtgat gccttgggag agcttgcgct
     1201 ggggtagtgt cctatatctt gcgcactttg gtcttcgccc gtccctgtgg cgatgatcgg
     1261 ttcgcttccc ctacggtctc accgagtctt gacatgtcga ggtagcggcg cgtcgaccac
     1321 tcgttcgacg tgatataacg cacgcgtgcg gacgagcatc aacgcgctgc gtccgtcggg
     1381 gaagctgccg accacgcgcg tgcgccgtct gatctcgcgg ttgcgggggt tgaccctggc
     1441 gaggacgttg cgttcgaagt gcaccatgca cctctggtag cgggcatcgc gttcaaggtc
     1501 tccttgacgc tcttgcggac catacggtcc agggtggtct ccggcatggc ctcatatcgc
     1561 aaaaaccgta cgctgaaagt aactcagcgt acggttttca cgaaagcagc catcgactat
     1621 cggtcagccg atagacctca gtcctcagtc gcagtgacct tggcgtcagc cccggccttc
     1681 tgctcaaggc cgagcttgga gcgcttgcgg ctgtagccga agtacacgat caggcccaga
     1741 ccgaaccaga cggcgaaacg cacccaggtc tcccagttga gaccggcgat gagcacgaag
     1801 cagaatacga tggccagaat cggggtgacc ggcacgcccg gcgcgcggaa gccgcggggc
     1861 gcattcggct gggtcttgcg catccataga ataccggccg acacgatgat gaacgcggac
     1921 agcgtaccga tgttcaccag ctcgaacagc acgttgatgt tgatgaagcc gccggcgaac
     1981 gcggtcagca gaccgaagaa ccaggtgccc ttgaacgggg tacggtactt ctcgtgcacg
     2041 tggccgaaga acttcgggaa caggccgtca cggctcatgg cgtagcagat acgggattgg
     2101 ccgtacagct gcacgagcat caccgtggtc atgccgatga gggcgcccag gttcacgatc
     2161 accgcgagcc agttcatgcc ggtctccaga atcacgccgg ccaccggggc gtcgatgtac
     2221 ttggcgaact ccttgtaggg caccacgccg gtcatgatca gggtcatgat gacgtagagc
     2281 acggtggaaa cggccaggga cagcagaatg ccgcgcggca gcgtcttgtt cgggttgatg
     2341 gtctcctcag cggaagagga cacggcatcg aagccgatga agctgaagaa cacgatggaa
     2401 gcggccggca cgatgccgta cggctgggtg gagccgggct ggaaagtgta gatgccgtac
     2461 ggggagaacg gctgccagtt ggacggcttg acataccaca cggcgcacac gatgaacagc
     2521 acgatgatgg cgagcttgat gagcaccatc acgtcattgg tgcgcttggt ctggttgatg
     2581 ccgatggaga gcacccacgt gatgatgagc acgatgacga aaccgggcag gttgaagtag
     2641 gtggtgacgt ccgggttggt gccgtaggcg gcggtcagct cgaccggcag gtgcaggccg
     2701 aagccttcca gcagtttgtt gaagtagccc gaccagccgg cggacactgt ggccgcctgc
     2761 agggcgtact ccagaatcag atcccagccg atcacgaagg cgatgagctc gccgaacgcc
     2821 agataggcat aggagtaggc cgagccggac accggggcca tggcggcgaa ctcggcgtag
     2881 cacagaccgg caaaaccgca gcagacggcg gccagcagga acgatacgga cagggccggg
     2941 ccggcggtca gcgcgccctt gccagtgagc acgaagatac cggtgccgat gatcgcgccg
     3001 atgccgagca tcgtcagatc gaaggtgcgc atcgtgcgct tgaggggagt ggattcggcg
     3061 acaagctgat ccacgctttt cttacgaaac agatccatgt tcttctctca tgaaatctag
     3121 gattatgggg ctgaaccgga cggatttccg ggatcccggc acgcctcaac gccgggaatc
     3181 aaaggcggct tccgagaaac cgcatatcgg tcattagtgt acgactgccg ggcgaaaaca
     3241 aacgattgtg accacacgtc acattcctgc gcaacaatag gtgcctatga gcatgaacgc
     3301 agcacccgaa atcgcattct cgtccgagca gggcaaggcc aactatgcgg ccgcccgccg
     3361 ccaatacccc gcacaggcca tcgtgaacct caaaaccatg cgcgacaaca tggcccacct
     3421 ggtctccgtg gtcggcggcc cgaactccgg cacggcggtg atgggcgtgg tcaaggccga
     3481 cgcatacggc cacggactgc tgcccgccgc actggccgcg ctcgccggcg gcgcgacatg
     3541 gctcggcacc gcgcaatcgc atgaagcact gctgctgcgc aaactcggca tcggccccga
     3601 ccgttgccgc attctgactt gggtctacaa cggcaccgaa gtgccattcg gtgagctgat
     3661 cgctgccgat atcgacgtat cggtcggctc cctgcccggc atcgatgccg tggccgccgc
     3721 cgcacgcaag ctcggcaagc ccgcccgcgt acacgtcaag gtcgattccg gcttcggtcg
     3781 caacggtttc acgccggccg gattcgacgc cgcgctcgcc aaactcgtgc cgctcgccaa
     3841 agagggcgtg ttgcatattg tgggtcagtg gagccatctc gccgtggccg attcgcccga
     3901 tgtgcccgaa ttcgtgtcct ccaccgaccg ccagatcgaa acattcaagg acttcacgcg
     3961 ccgcatggaa caggccggca ttccgccgga gatccgtcac ctggccaaca cagcggccac
     4021 tttggaccgc ccggagattc acttcgaact cacccgcccc ggcatcggcc tgtacggcta
     4081 cgagcccgat cccgcgatgg gcaccccgcg cgactggcat ctgaccccgg ccatgcgcct
     4141 gcaggcgcag ctcggcacgg tcaaggatgt ggaggccggc cacggcatct cctacggccg
     4201 cacctacctg accccggaca acacctcgac cgctattgtg ccgctgggct acgccgatgg
     4261 catccaccgc tccgcttccg gtttcgatat ggaaggtgcc aagcacgtgg agaagccggg
     4321 cggcccggtg cgcatcatga ccaccgaagg cccgaagatc taccgcgtgt gcggccgcgt
     4381 gtgcatggac cagttcatcg tggacctgca cggatccgcc gccgaactcg gcgttcacga
     4441 gggcgacacg gtcgaactgt tcggcccggg ccgcggcgag gattacgttg agcctaccgc
     4501 cgacgattgg gcccgcgccg ccgacaccat cagctacgag atcttcacct gcctgcgcaa
     4561 ccgcatcccg cgcctttacg agcatgccgc cgaagtgctg cccgccgccg acctcgccaa
     4621 actcgacccc gccacgctgc tataaggtgc attaacgcca tacggtggca ccccgtaatc
     4681 ccttcttccc gaatatgtgc cactgatatg tgctgagcgt cacatattcg ggaaatcggc
     4741 cccaatctcc cgaatatgtg ccgttgagtt gacgtcagtg gcacatattc gggaagtcgg
     4801 gattactgcg gccgatatgt gtgcctatgc ttggcatatg gaaacgactt tggatgggga
     4861 acaggtgctg agcgacgagg gctacagcgc cttcgacgag gagcgatggg cacccgagcc
     4921 gcccaaatcc aagtcacgca ccgcgttcga gcgcgatcgc gcgcgactga ttcattcgtc
     4981 cgctttgcgg cgtttgggcg cgaaaagcca gattctgatt gccggtaccg acgatttcgc
     5041 tcgcactcga ctgacgcata cgctggaagt cgcccagatc ggtcgccaaa tcggcgcatt
     5101 gctcggctgt gatccggacg tggtcgactg cgcctgtctg gcccacgacc ttggtcaccc
     5161 gccgttcggg cacaatgggg agcgtgcgct cgccgacatc gccggcaata tcggcggctt
     5221 cgaaggcaac gcacagacca tgcgtatcct gacccggctc gaacccaaga tctttcaccc
     5281 ggacgggcgt tcggccggcg tgaacctgac ccgtgcggcc ttggatgcgg ccgtcaaata
     5341 cccgtggacc ttggccgaag ccgaccgaca cccgaagggg gagcggtcga agaaattctg
     5401 cgtctacccg gatgacgaac cggtgttccg ctggctcaaa atcggcgcgc cccaggccgc
     5461 caaacccatg gaatgccaga tcatggacct ttcggacgat atcgcctact ccgtgcatga
     5521 cgtcgaggat tccatcgcca ccggcgcgtt cgatccgatt gtgctcgccg atccgaagat
     5581 gctggaccac atcatcgagc agacgcgcgc atggtatggg gcgaagtggg atgccgacaa
     5641 gctgctggcc gcgttcatgc gattgcgccg agagcatttg ttccctgcac atttcaatgg
     5701 ttcgcgcgaa tcgttggcgc agcttaagaa cattacgtcc gatttgatcg gccgcttctg
     5761 ctggtcggtg gaaaccgcca cgcgcgacac gtatgggccc gggccgttga cccggtattc
     5821 ctcgaacatc gtgattcccg agaacacgaa ttacgagatc gtggcactga agggcatcgc
     5881 cgtgtacttc gtcatggcgc ctcgcgagcg cgagccattc catcaggagg agctcaagat
     5941 cgtatcggat ttggtcgatg tgcttatggc cgattcgccg ctcccatccg acgctttgga
     6001 aagccagttc ctggccgatt ggaatgaatc caccaatgac aacgagcgcc tgcgcgtcgc
     6061 catcgatcag gtcgcaagcc tgaccgacaa ctccgctctg gccctgcact ccatcttgtg
     6121 ctgagggcgt gttggacggg tgcttccctc agtctcgctg cgcgagccag ctcccctcgg
     6181 gtgaggggag ctgtcgctgt aggcgactga agggagtttc gcggaagaaa tgatgaccga
     6241 cggtaaaaat ttaccgtgtc tcaggtgagg cggtactcta gagacttatg gtcggaatga
     6301 ttaagaaaga agatgtcgag aaggtgcgcg ccgccgcgga cctgtatgac attgtatctg
     6361 cgaccgtaac gcttaagccc tccggcaccg gcaccttcgt aggcctgtgc ccgttccacg
     6421 acgagaaaac cggcagcttc aacgtgcgcc cctcactcgg cgtgtggcac tgcttcggct
     6481 gcggtttggg cggcgacgta ttcaaatacg tcgaacaatc cgaaaacatc gacttccgcg
     6541 aagccgtgga actgctcgcc gacaagtacc atatcgaact gcactacgag aacagcggca
     6601 acgggccgaa ccgcgaaaac gccggctcca agcgcacccg actgctggaa gcctgcgagg
     6661 aagcccaacg cttcttcgtc tcccagatcc tgaccaagga agcactgccc gcccgcaagc
     6721 tgctcggagg ccgcaacttc tcccaagccg actgtgaacg cttcggctgc ggctacgcgc
     6781 cccaaggctg ggataatctc gtgcgccact tggcctccaa gggatttacg cagaaggaaa
     6841 tcctcgacgc gggcttggcc cgtcaaggcc agcgcggcat ctatgactat ttccgcggcc
     6901 gagtgacctg gcccatccgc gattcgaccg gccgcacgct cggcttcggc gcgcgcaagc
     6961 tgtatgagga cgaccagatc gccgccaaat acatcaacac gccggacacg cagctctacc
     7021 gcaaaaccca ggtgctctac ggcatcgatt tggccaaatc cgccatcgtc aagaagcgac
     7081 aggtggtgat cgtcgaaggc tatacggacg tgatggccat gcacttggcc ggtatcgaca
     7141 ccgccattgc tacgtgtggt accgcattcg gcgccgagca cgccaagatc gtgcgccgcc
     7201 tgatcgccga cgattcgctg ggcgcggtcc agctggtcgg gccgttgaag gtcaaggacc
     7261 agccgttgag ctcgcgcatc gtgttcacgt tcgacggtga cgccgccggc cagaaagccg
     7321 cgctgcacgc tttcggcctc gattcggcct tcctctccca aaccttcgtg gccgtggccg
     7381 acgacaatct cgacccgtgc gacctgcgca tcgagcgagg caacgaagca gtgcgctcgc
     7441 tgatcgcccg tgccaaaccg ttgtatgact t
//
LOCUS       DAWBMW010000072         7421 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_2312_length_7421_cov_2.644312, whole genome shotgun sequence.
ACCESSION   DAWBMW010000072 DAWBMW010000000
VERSION     DAWBMW010000072.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 7421)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7421)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7421
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2312_length_7421_cov_2.644312"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3302..3311
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4964..4973
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1476 a         2162 c         2262 g         1501 t
ORIGIN      
        1 cctcagaacg ggtgttctgg gagtaggtct cggcatccgc gtgagtctgg tcacggtaag
       61 cgtcggcctc ggagcgggta cgatccgaat aggtgtcggc gtcggaacga gtacgagtgg
      121 agtagtcgtc ggcctcggaa cgggtacgct cggagtagtt gttggccttg gtgaccagat
      181 cgttgtactt gttctggctg gcctcggtga tttccttggc cttgttcttg cccttctcca
      241 cgtactgatc gtgcagctgc atagccaggg tcagcatggc ggtggcgcgc tcgggctcgg
      301 tgcccggcat ggaagcggcg ccagcgatct tctgcagaga accggtctcg gtgctcggct
      361 caaccttgga aacctgagca cgaagctggt tttcacgctg cttggcttct tcgagctgac
      421 gggacagctc agcgatctga gcggactgct gctgagcggc ggaaccctgc tgctgagcgg
      481 cggcgagctg acgggacagc tcctcgttgg ccgcgcggaa ctgatcacgt tcctgctgaa
      541 tggcggcgat ctgctgaccg acagcctcct ggtcaccgga ggcctgagca gcctgagcgg
      601 tgagctgatc gatttgagcg ttcagctgat cgacctgacc cttgagctgc tcattctggg
      661 aggacagcgc gcggttggat tcctcggctt cgctgagctt ggcagagaca agctgtgctg
      721 cctgatcatt gccagcctga gcggcctgct gggcctgagc cacagcgttc ttcaaaccag
      781 cattctccga ctcgagttgc tgcacctgac cggtaagatc ggagatcttc gcattaaggt
      841 tggtcacgtc aggacccaga gactgggtgg cctgaccgcc ggctaccgcc tgacgtccaa
      901 gagcctcgac ggtttccgtg acctgatcaa ggaagtcatc gacctcgtcg acatcataac
      961 cttccttgaa tcgaacagtc tggaaggtat gctccctaat atccttcggc gtcaacagag
     1021 ccataaatct tgcacctcgc tttggggcat cgcccgcact tacgttggtc tcaagtcaga
     1081 atgctatcac gaccccatcg tttcagaaca ttcgacgtgg gtttttatgc cagatatatg
     1141 cctccggcgg gtcaaaaacg gcctcagaac actatcagaa caacactttc cagtaccacc
     1201 agcgcaaaat acagcacaat gaagctgaca tcaagataaa tcgaaccgag cggcaatggg
     1261 cgaatatagc gacgtagcca gcgcagaggg ggttcggtaa tggaatacac aatattgatc
     1321 agctgggcca ccacaccacg tggataccag tttcgtgcga gaacggagac ccagtccaga
     1381 atcatgcgca cgaacagaac cgtgatatac gcgttaatga gccatgcaag aatgcggccg
     1441 atcaaagaga acactaagga aagcatgctg acagactaac aaccgcgcat gaaaagtttg
     1501 ctcggagcac aataagaatc ggccgactcc ttgctgggag tcggccgaat accgatataa
     1561 cgtcgaatgc gaaaactact gaaccggtca gtcagcgaac aaatcgtgtg ccgaggctgg
     1621 ttttgtcggc tcttcgacct tgatgttcac ctgcgccggg ctcaacagga acacgcgcgg
     1681 agtgacacgc tcgagggaac cacgcacgcc gaacaccacg ccggcggaga agtccacgat
     1741 gcgatatgcc acggcttcgg ccacaccggt gagattcagt acgaccggaa caccgtcacg
     1801 caatgcgcgg ccgaccagtt gagcatcctc atatgacttc ggatggatgg tggtgattct
     1861 gttcacacgg ccaccgggga acggcttggt agtcgacgat gcggcaggcg cggtggttga
     1921 cgccagcggc gtgaccgtat ggtcggaatc aaatgcggac ttcgaagccg gcttctgctc
     1981 ctcgtcctcc tcgatgtagt cgtcctcatc gtccacaacg tcactcatgc ccagatagga
     2041 catcgcgttt ttcatgaacc cagccatgga tcaatccttt cagcgcagga agtcagggat
     2101 gtcgagatcg ccagccggat cgttcacggc gatattcgga tgagcggaag tcggatcatc
     2161 aaaaggcaac gatgcggagt caccggtagc cggtgtgaat gccggttggg cagtggcagc
     2221 gggctgtgca gccggtgccg gtgctggagc tggctgtgcc acgggagtcg caggatcggc
     2281 ggcggttggc acaaccggcg tcaccgtgct ctgagtgtcg tcatcttgag cggcgacggg
     2341 gtcaaaacca gcagcaataa cagtgacacg cacttcatcg ccataagcat cgtccagagc
     2401 aagaccccaa ataatctggg cttcagggtg aatggccttg cgcaccagtt cggtggcagc
     2461 agaggcctcc tgcaacttca ggtcggtcgg accggcgatg ttgatcagcg caccgtgtgc
     2521 gccttcgatg ctttcctcaa gcagcggaga gctgatggcg atttcggcgg ccggcgtggc
     2581 acgatcctct ccccgtgcgg aaccgatacc aaacagcgca gtaccagcac cacgaagaat
     2641 ggagttcaca tcgttgaagt cgacgtgaat ataggagttc atcgaaatca ggtcagtgat
     2701 gccctgaaca ccggcaagca gtgcggtatc agcggtcttg aaggcctcaa tgatgccgat
     2761 ggaacggtcg gaaagctcga gcaggcgatc gttcggaatg acgatcagcg cgtcgacttc
     2821 cttgcgcagg ttgtcgatgc cgtattcggc ggaagcggaa cgctgagggc cttcgaagct
     2881 gaacggacga gtaacaaccg cgatggtgag agcaccctgt tgatgagccg cacgagcgac
     2941 gatcgggctg gcgccagtac cggtgccacc gccctcgccg caggtgacga acaccatgtc
     3001 ggcaccccta agcgcctctt cgatatccga ctggtggtcc tgggccgcct tggctccacg
     3061 ctccgggtcg gcaccagcac cgagaccacg actggactta tcggaaaggg aaatcttgac
     3121 atccgcatca gagcgcagaa gatccttcgc atcggtattt accgccacaa attcaacatt
     3181 ctgtaatcct tcggcaatca tgcgattgac ggcattaccg ccggcaccgc cgacgccaac
     3241 aaccttgatg ttggtcttgt cgttgaactc attctgcgcg atctcgctca cgtttggctc
     3301 cnnnnnnnnn ngttacaccc aagagtagcg caaacaggct actcttggag ggaaattcac
     3361 gcgcagaatg gcgcgtgttg cgtgtatttt cagtattttc tcagtaactg gcgtccattg
     3421 ggtcgtcacc gaagagcgcc tcacgctctt cgtgggtggt ggtacgcgat tccaaccagc
     3481 catatggcaa gtgcaccttc ttggcgaaac gcacgcggcc gcgcggtttg tcgaccacgc
     3541 gtggcgggta ttcgatagtc gggtcgagct tgccgatctc gcggtccaca tcggccaggc
     3601 tctcgcattc cataaaggcc ttgcgggtgg agccgcccac cgggaatcct ttcagatacc
     3661 aggcgatgtg cttgcgcaga tcgtggacgg ccatcatctc atcgccctca taaaactcgg
     3721 taagcagctc ggcatgacgg tgaatcacgc ggcagacctc gcccagattc gggttgagcc
     3781 gttcgggatt gccctcgaag gcgtttttga tgttggcgaa cagccacggc cggccctggc
     3841 atccgcggcc gatggccaca ccgtcgcagc cggtctcgcg caccatttcg agcgcatcct
     3901 cggcacccca gatatcgcca ttgccgaata ccggaatgtt gaggtgttcc ttgagctcgc
     3961 cgatgcggga ccagtcggaa tgaccgccat agtattcggc ggtggtacgg gcatgcaggg
     4021 ttacggcctt gcagccctcc tcctgcgcga tgtgtccggc ttcgaggaag gtctcatggt
     4081 tatggtcgat gccgacgcgg atcttcgcgg tgacggggat gcccgccggc tcgcatacgg
     4141 cgaccacgcg ctgtaatagc tcagtgaaaa tgtcggtttt ccaaggcaag gccgaaccac
     4201 cgcctcgacg agtgaccttg ggcaccgggc aaccgaagtt cagatccaca tggtcggcca
     4261 tgttgccgtc gaccacgatt ttcgccgcct gttcggtgat ggaggggttc acgccgtaca
     4321 actgcagtga acggattttt tcgcttttgg cgaaatgaca gaggcgcagt gctttcgggt
     4381 tatgcgccac gagggctcga gcggtgatca tctcggccac gtagagtcca tcgggaccgt
     4441 attcctcgca aatcacgcgg aagggccagt tggtcactcc cgccatcggc gagagcacca
     4501 ccggggtcgg cacggtgatg ttgccgagtt ggaccggggc gatatgcacc ggggtcccgg
     4561 ggcgcgccgg cgcgtccgta cgtgggtcga ggttgtcggt ctcgtgatgc tcggtgagca
     4621 ctgtttcgga ttcggattgt gccatgtgat tcctaagaaa ataagtaggg gctcccctga
     4681 gagaggagcc ccggcgtcta tcgccgccac ttcgcctaca aacagcccac tgggatgtct
     4741 tccttacggc tcagcccaca gaggggagcc caagttgtgc gactccggct tagtacaggc
     4801 cgccggcttc ctgaacgccg gactcaggcc aaggctcacc gcccatttcc acaggcttca
     4861 ggggcacgcg ggtgcgcttc tcgccgatgc gggcggccac gtaatcggcc accttgtcaa
     4921 gagcgacgcg ctcctgggcc atggtgtcac gctcacggat ggtnnnnnnn nnngccacgg
     4981 tctgcagttc aggcttcttg ctcagcggca gcacggcggc cttgaccggg gccagacgcg
     5041 ggtcgagacg cagcacggtg cgcttgtcaa cgccgccctt ggtgttcggg gcctcgtcca
     5101 catcgtaggc gtccacgagg aagcacatca gggagcgggt caggccggcg gccggttcga
     5161 tgacgtacgg cacgtacttc tcgccggtgg cctggttgaa gtagctcagg tcttcgccgg
     5221 agtgcttgga gtgcgcgctc aaatcgtaat cggtacggtt ggcgatgcct tcgagctcgc
     5281 cccagtcgga accctggaag ccgaacttgt attcgatgtc cacggtgcgc ttggagtagt
     5341 gggagagctt ctccttcgga tgctcgtagt ggcgcaggtt ctccggcttg acgccgaggt
     5401 cgatgtacca gcgggtgcgg gcgtcgatcc agtactggtg ccattcctca tcggtgccgg
     5461 gcgtgacgaa gaactccatc tccatctgct cgaactcacg ggtgcggaag atgaagttac
     5521 ccggggtgat ctcgttacgg aaggacttac ccatgttggc gatgccgaac ggcggcctgg
     5581 aacgggaaga ggtcatcacg ttcttgaagt ccacgaagat gccctgtgca gtctccggac
     5641 gcaggtagtg caggctgttc tcgtcctcga ccgggccgag gtgggtgcgc agcatcatgt
     5701 tgaagtcgcg cggttcggtc cactggccac gggtgccgca gttcgggcag acgatgtcct
     5761 tcatgccgtt ttccggcatc ttgtcgccgt gcttctcggc gtaggattcc tcgagcttgt
     5821 cggcgcggtt gcgcttgtgg cagttgagac actcgacgag cggatcgttg aacacgctca
     5881 cgtggccgga cgccacccac acttcgggcg gcagaatgat ggaagtgtcg acaccgacga
     5941 catcaggacg ggtgacgacc atggagcgcc accattcacg cttgatattg tccttgagag
     6001 cgacgccgag agggccgtaa tcccaagcgg aacgggtgcc gccgtaaatt tcgccggcgg
     6061 ggaacacgaa gccgcgacgc ttcgcaaggg atacgacttc atcgagtttg gactgagcca
     6121 caatgcacct tctggtttga acggtttggg gtctaataac aaccgcttca ggatagccgt
     6181 atatggtgac aggtcaggcg gcattgtccg gtatatgttc cgcgtctcac gagcggccgt
     6241 tcgcagcgat tcctatatcg caacacctcc atattgttcc tgttgtttta cccttcccga
     6301 atttgtgcca ctgacatcgc gtcagcgtca caaattcggg agatgttgct cggcggaggc
     6361 gtctatgtat cacgaatgga cggggacgcg ccagacctga cgacacgtat cgaacaaatc
     6421 cgtcacgata atgtccatgt tggcgcatag cgtcggcact gtaacgcagg ggaactcgca
     6481 tgtggttcgc gagttgagaa aggcctgagc ctgaccctta gaacctgttg gttaagacca
     6541 tcgtagggag cagtaaatga gcaatagtgc atcatcgttt accggcgtgt ccagcggtta
     6601 taccgccggg actccggttc cagccgattc acccatccgt gacaatatcg ccgatgccgt
     6661 tcgccgcgta cgcgagacga ctccgttggc ccagtcattc accaattttg tgaccatcaa
     6721 tttggttgcc aacgcgcaac tggcggccgg cggcactgcg gccatgagtt tcctgccgga
     6781 cgacgtgatc gaaaccgcga aaatcgcggg cgcgaactac atcaacgtgg gcacgctgct
     6841 gccgttctat aaggacgcac tgcccgaaat cgcccagcga ctcaactact tggacaagcc
     6901 gtgggtgctc gacccggtgg ccgccggcat cggccgcacc cgtaccgcca ttcttcaggc
     6961 cttcaaggcc gctcccccga ccatgattcg cgcgaatgca tccgaggtaa tcgcgctggc
     7021 gaacatgtgg gggctcaata ccgaaaccgt gggggacgct tccgagcacc gccccgccgg
     7081 agtggaatcc gtcgacgacg tggagtccgc aaccggcgcg gcggtggcgc tggcacagta
     7141 tctgaccgaa cagcacgcca agcattcctc ccatgacgct tccacccgct gcgccgtggc
     7201 cgtgtccggc atcgccgatc tggtgaccga tggcgaaacc gtgtaccgac tgccgggcgg
     7261 cagcgcgatg atgaccaaga tcaccggtgc cggctgctcg ctgggcggcg tggctgccac
     7321 gtatttggcg gtctccgatc cgttgaccgc cgccctgtcc gcgtctctgc tgtacaaccg
     7381 cgcgggagag gtggccgata caacttcgta cggaccgggc t
//
LOCUS       DAWBMW010000073         7368 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_2335_length_7368_cov_2.432928, whole genome shotgun sequence.
ACCESSION   DAWBMW010000073 DAWBMW010000000
VERSION     DAWBMW010000073.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 7368)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7368)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7368
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2335_length_7368_cov_2.432928"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1647..1656
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1888..1897
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1474 a         2012 c         2265 g         1597 t
ORIGIN      
        1 gaacgcgagc atgcgagcat ccatcagatc tgcggtgaac gtcaatacgg gaaacggaat
       61 cagcgccgcc gcaaacgaca tgaaatgcgt tttcaccatc gaccagtcga ccggatcgac
      121 cggcggtcgc cggcgtttcg gcgtgaacag atggcctcgg ctgaccgtta tagtgatgat
      181 ggacacagca aagatgacgg cgaccatcca cagaacgccc ggcaacaatc cgacaacaat
      241 cccgttcacc cggtcctcca atcagtcata ataatcaccg caacatataa aaacataaga
      301 tggcaggcaa caaaagcaac acatacaaca aagcgacctg ccgaccaaag ccggcaagtc
      361 gccaacaacc atacatgtat caggcggttg cgcgttgcaa gcgctgtgcc tcgagcttct
      421 tgcgcatcct catggcagtc agttcatcaa tcggacggcc cgtggcaatc gtcagcggct
      481 tggcctcaag atcaacatat tcttcctgtt ccaaggccgt ctcaatctgg tgccacaacg
      541 aacccaccga cacggcgaac actgccttac cgctttgcaa cagttcgagc atctgttcgc
      601 tagttgtgca ttcatgtacg ttttgaccat cgcacatgat ggtcacattg gccagctgat
      661 cggtgctgcg ttgagtcaag aatccgatgg ccgccgttac gttctgcaga ttcacaccgg
      721 catcaagcaa cttcttggat acagcgagaa tcaccacatc cttaaacgag tatagtcgtc
      781 gtgaaccgga gccatgagac ggcgtgatgg acggctcaac aatctgcttg cgtgcccaat
      841 aatcgagctg tcggtacgtg atgccggcca ccttggaggc cacagtgcca cggtagccgc
      901 gcttgatctc ctcagtatcg acgttggaga acaactcccc ctgaatcgcg tcagcgttca
      961 gattctgacg gtcctcaagc gaaacatgaa gatgcagccc ggtctgtccc atggcactcc
     1021 cctaactcat gcgtgtctcg catatgactc acactggtgt aatcacatga ttgcaatttc
     1081 tgcaatcagt ctaaccccgt gctgggtgtg gactattggg aaataatggc agatttcgtg
     1141 aagaacacga ggcggaattt accgatcatg atttcgtcgc cgttcttcag cgtcgcctga
     1201 tcaacacgct gacggttcac gtaggtgccg ttcagacttc ctgcgtcaac cacaaagaac
     1261 tgaccgttga ccctgcggaa cacggcatgt tgacgggata cggtggagtc gtcgagcaga
     1321 atatcagctc gcggatcacg gccgacggtg atttcatcct cgtcaagcaa gtaacgagaa
     1381 cctgagaccg ctcccctggc ggaaatcagc aatgccgttc cctcggacaa tctggtgatg
     1441 gtgtccagat cctcttgagt cagcggtcga tcgccggtgg tggtaatggg cagtacatta
     1501 atcgcgggca ggccgataat ggtggtttcg cctgcgctag gaatcgggtt ggtcataatc
     1561 ccctattcta ctgtctttgc atactggttt tgcgtagctt cgcgtacttc gtcgataatg
     1621 atgctgtcag aaggctgtac attgacnnnn nnnnnnttcc tgcggattgc caatggcctt
     1681 caccgtgtat gtgggcttga tggtggtgcc atccacgatc aaaccttcct cggcgtcctg
     1741 aatgtaggtg gaggtgatga cgcgcacgtt attgatggcg attacctcgg caccggcatt
     1801 gcgaagttcc tcgataaggt tgaacatgat tgaggcgtcc actttagtcg tcaagcctcg
     1861 tccgattcgg atggtcacgc cttgaccnnn nnnnnnngtt aaactgggtt ttacgctgct
     1921 cgagattgga gatttgcgta ttcgactcgt tcagcagcct taccagttca ctctcgtcca
     1981 tcgtttcgta cgtggattta gtgttattga tctggctcat gtaggcgaaa cccaacaacg
     2041 cgcacatcag agcgatgaga atcgaggata gtaaccgtga tttgttgctg gcacggttga
     2101 gatccttccg gggcttcttg cgcaccgtgg gaaacgaccc ggtttccatc cggtcgttga
     2161 ccttgtcttg tgcatgctgg tcgcgcattt tctccagcat gttgtctgat tgtgctttac
     2221 gtgccatacg tcagtaccct tgtcagcctc ggaagatgaa gcgtctgatc gcggacacgt
     2281 tggagaagat gcggatgccg agtacgacga tgacggctgt ctgcagctgc gaaccgacgc
     2341 cgagctggtt gccgaggaac acgagcagtg ttgccgtcac cacgttggcg aggaaggaga
     2401 tcacaaacac gcggtcggag aagctgcgct cgaaataggc tcgcgcggca cccagcaggg
     2461 cgtcaagagc agcgaccacc ataatcggca gataaggctg cacgatgatc ggaatgtccg
     2521 gcttgacgaa cacgccgatg acgacgccga tgatcagtcc cagaacggct gccattattc
     2581 attcctttcc gcgtacttca cttcgccagt gactgctgcc tcaagtgtaa tcgaattgga
     2641 tttgctcacc tgcgggtaga taccggcttc cttgaaggaa tcgtaaagac ttgcaagttt
     2701 cttcttcccc atcgcttcgg ccaaggcgct cttatcgcct atggcttcaa tggtataggg
     2761 gctttgtatc gcggtcatgc cgatgagaat atgcccgccg gccttgcgta tcgaggtttg
     2821 cgcgccgagc ctattaccgt tgatggcaat ggcttcggct ccgttttgga acatcaggga
     2881 gaccagttgc tgcagatcca gatcggtgat gactcggatg ttcgtggagg tgccgactct
     2941 cgtcgaggag gattgatccg actggcttgc cgcgattgga tcggccagcg tcatggtgat
     3001 tccttcgcct tcgaccaccg tggcaccatt gaccatttcg tcttggataa gcgtatcgct
     3061 tttcacgctg ccggatattt tcttggactg ttcgtcaact tgggagcgca aattattgac
     3121 gtccgtggtg agttcgtcta tctgcgagtt gcgattctcc aattgcgtgg ccaaagtctg
     3181 ccgtacctgc ttacgcggat cggattgcaa ctgttgcacg aataggctgc aggcaaagcc
     3241 aacgccgacg cagatgatga ataagacgat gcgattaaac cagatggaga aggtcgaacg
     3301 atgctcatgg accagtcgtg agtcggagaa catcggatcc atcggacgat tgacaagatc
     3361 atcgatgagc tgaagcgaat cgtcgttggt tttacggcga cgtctggtgc ggatggcggc
     3421 ggcgtcatcc ggggaaagcg catgatgtga ttccgcgcct cggactgagt gggagaacac
     3481 ggcgcggcgt cgtaccggct cattctcctt gggtacgggg aatgagaccg gggccgtggc
     3541 ctcaagcgcc atacttctcc cccagttcct tgcgtagcaa tctgatgccc tgctcggtat
     3601 agatatatcc ggcgagccaa tacatggcga tgccccagat gccggcggcc agcgcggcga
     3661 tgtagagcaa gtggaagaac gtattggtgc ccaaatggaa atcatcagca gcgcggtacc
     3721 ggccttgccc acgaaatgca cgggcagcgg accataaccg tactgggcaa gccatagcac
     3781 ctgaatcgcc atccacagat ccctcagtcc cacgataatc agcatccacc atgggatgat
     3841 gccagcgacg ctgagggcca gaatcgagca gaaaatgagc agtcggtcgg caatcggatc
     3901 aagaatctga ccgatcttgc tcacttggtt gaatctgcgc gcgatgatgc cgtcgagtcc
     3961 gtccgaagcc gaggaaatgg cgatcagggc taacgccgca atcatctcgt gcttggagat
     4021 cagcacgctg atgaaaggaa tcgagatgat tcgcagcgcg ctgatcaaat tgggcacggt
     4081 gaaatagatg tcacgtgctt ccggactgta tctggtgttg actttctggt tggtcatatc
     4141 gactaacagt ctacatgcgg gaggcttgta gagcggtaac gatgatgccg cgtgcgccga
     4201 cttcgtacaa atcatccatc agctggttga ccttggcctt gggaaccatg actcgcacgg
     4261 cgttccactg tttatcgtgc agcggggaga tcgtcgggct ttcaaagccg ggggtcacgg
     4321 ccacggcggc ggcgaccttg ctgatcggaa tgtcatagtc catcagcaca tactgatgtg
     4381 cagtgagcac gccttgcaga cggcgggtca gcacggccag acgtgagtcc tgctcgttca
     4441 gacgcggcga acgaatcagg caggcttcgg agtgcatgag cggttcggca aacacgcgca
     4501 ggccggcgtt gcgcagggtg gtaccggtgg agaccacgtc ggcgatgagg tcggccacgc
     4561 ccagctgcac ggaggattcg accgcaccgt ccagatggat ggtctcggcc tggatgccgt
     4621 gctcgaccag atagtcgtgt acgagcttat cgaatgtggt ggccacgcgc ttgccctgaa
     4681 tatcctccag agtggtgatc ggggattcgt tcggtgcggc gaaacggaag gtcgaggtac
     4741 caaagccgag cggcatgtgc tcgagcgcgg cggtatcgga gttcttgagc agatcctggc
     4801 cggtgatgcc gacgtcgatg gcgccgcggc ccacgtatac ggcgatatcg agcggacgca
     4861 ggtagaacag ctcgatgcca ttgtcgggat cttgcacgac cagctggcga gggttggtgc
     4921 gcagacggta gccggcctcg gccagcatgt tccacgcggg ttcggatagc atgcccttgt
     4981 tgggcactgc gattctcagc atttgcgttc ctctcacagg tgcttgtaga cgtcctcaag
     5041 cgtcaggccg tgcttgatca tcatgacctg gacgtggtag agcagctggc tcatttcctc
     5101 ggcggtgcgg tccgcaccct cgtattcggc ggccatccag gtctcgccgg cctcttcgat
     5161 gatcttcttg ccgatgaagt gggtgccctt gtcgagctcg tcgacggtca gggagccttc
     5221 ggggcgggtc tgggcctttt cggacagttc ggcgaacagg gattcaaacg tcttcatgtg
     5281 attcagattc ttttcttgcg gttatggatg aaaggtggat tgcggttcag gcttggagct
     5341 caggccttga acgcggcctc ggccttggtg cggatgtcgt cgatggcttg ggcgggattc
     5401 tcggcgccgt acacggcgga accggcaacg agcacgtcgg cgccggcttc ggccacgatg
     5461 tgcgcggtct tggggctcat gccgccatcg actcgcgcag ctgtcgggcc agacgcacgg
     5521 gggcgtgggt ggcacccatg tggaaggaga tggaggcggc gccgagcttg gcgtattcgg
     5581 gagcccaacg gtccgggtcc tcgatcatga ggtgcacgtc gaccggcaga tcggtgacct
     5641 gacagatgcg gccgacgatc ggctcgccca gggtcaggtt cggcacgaaa tgatggtcca
     5701 tgacatcgac gtggaccagg tcggcgttgg cgatggcctt gagatcacgt tcgaggttgc
     5761 agaagtcagc ggacaggatg ctgggtgcga ttgcgatatt gctcatggtt tcctacctta
     5821 tctgtgcaat gggacttgga gccccgtgtt tcgcgctgtt tcggcgctgt ttatcatggt
     5881 gtcacttagt gcgattggtt tgctttttct ggcgaagttg ttcctcttcg atgctctggc
     5941 gttcgagctc gtcggcggtg accgccgcaa gcccgcgcgc ctgcgccttg gggtccggcc
     6001 cgtactggta gagcacgacg aacaggatgc agccgagcac gaagacgatg atcgcggtcc
     6061 acacgttggt cctcaggccc aggatcaccg tggaatagtt gatgcgcacg ttctcgatcc
     6121 aggtgcggcc caggccgtac cacatcaggt acatggcgaa ctgctggccg gccttgagcc
     6181 ggtcggcaag cttgtggccg aggtagatga tgagcgcggc gccaatgagg ttccagatca
     6241 tctcgtacag gaacgtcggg tggaacagcg tggtcctgta gtccgggcat tgcgcgccag
     6301 aatagcagat ttcggatttg ccgatggcgt ccgcgtcgtt gagcttcagg ccccacggca
     6361 acgtggtggg ccagccgtag agctcctggt tgaaccagtt gccgagtcgg ccgatggcct
     6421 gggccacggg cagcgcgggg gcgatggcat cggcgaagat cgcgaacgga tagtgcttgt
     6481 gacggcacca caggaacgcc accaaggtgc cgacggagat gccgccgaag atggccatgc
     6541 cgccttccca caccttgagg atgttgacca ggttgccggt gggcgggaag tagtcggccg
     6601 gcgtggtgat gcagtggtag aggcgcgcgc ccaccagtgc gcagggcacg gtgacgagcg
     6661 tggtgtcgag aatctggtcg aaggtgccgc cgtaccgctt ccaacgtgtg gcgaggatcc
     6721 atacggccac gcagatgccg atcaggatgc agatcgcgta catgtggata gtgaacgggc
     6781 cgatctcgaa cttggagaag gtcggcgagg gaatataagc cagattcatc gtgcctctac
     6841 ttgctggtgc tcatggtgtg tgtccacgcg cgggttgtgc ttgacgctct gtgactaccc
     6901 taccgtgtca cggcacaacc cgcgtgaggc gcaccgggtg tttaccgggc gttgtggatg
     6961 ccttcggcca gttcctcgct cttggcggcg agcagcttca agccttcctt cgggtcgcgt
     7021 gcggtcttgt tgtcgtccgc gagcagggtg tgcacgagcg cggagccgac gatgacgccg
     7081 cccgcgtagg agccgacctt ggcgccctgt tcggcggtgg acacgccgat gcccacgcac
     7141 acgttctcgg caccggcctg acgggtgcgc tccacgagca gttcgggcga ggcgtcgatg
     7201 gtggcgcgtt cgccggtgac gcccatgcgg gcggccgcgt acacgaagcc acgtgcgttg
     7261 cgggccacgg tttcgaggcg ctcggtggac gagtccgggg agaccaggaa gatgcggtcg
     7321 agtccgtggc ggtcggaggc ttcgatccac tcccccgcct cgtccggg
//
LOCUS       DAWBMW010000074         7230 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_2390_length_7230_cov_2.396794, whole genome shotgun sequence.
ACCESSION   DAWBMW010000074 DAWBMW010000000
VERSION     DAWBMW010000074.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 7230)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7230)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7230
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2390_length_7230_cov_2.396794"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4644..4667
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1498 a         2253 c         2041 g         1414 t
ORIGIN      
        1 gccgcgcgca ccatggtcga aggctggaag gccgtccgcg gcgacaagaa gcacggctac
       61 gctccgcgcg tcgtaaccca cacccccgct actgacactc aggctgctca ggaaggggcc
      121 gcgaagcccg gttccgaggc cactgagaag aagttcacca acgccaagga cgccaaggat
      181 gcccagaagc ttgccaagca gcagcgcgtg gacatcgccg cccgtggttc caagcagcgc
      241 gacaaggcgc acatccgcaa gaccaagtcc gtaccgttca cctatcagta tggttcctac
      301 gacctggaag tgccgtacac cgaaatcaag ctgagcgaca cgcccggcgt gggcccgaac
      361 ccgccgttcc acgactacaa caccgaaggc cccaagtgcg acccgaagga aggcttgaag
      421 cccctgcgtc tcgactggat ccgcgaccgc ggcgacatcg aagactacga gggccgccgc
      481 cgcaaccttg aggacgatgg caagcgcgcc atcaagcgcg gccgcgccac caaggaatgg
      541 cgtggacgca agcatgagcc gatgcgtgcc aaggaccacc cgatcaccca gatgtggtac
      601 gcccgccacg gcatcatcac cccggagatg cagtacgtgg ccacccgcga gaactgcgat
      661 gtggagctcg tgcgctccga gctggccgcc ggccgcgcgg tgatgccctg caacatcaac
      721 caccccgagg ccgagccgat gattatcgga tccgccttcc tgaccaagct caacgccaac
      781 atgggcaact cggccgtcac ctcctccatc gatgaggaag tggagaagct gacgtgggcc
      841 accaagtggg gcgccgatac cgtgatggac ctgtccaccg gtaacgacat ccataccacc
      901 cgtgagtgga ttcttcgcaa ctcccccgtg ccgatcggca ccgtgccgat gtaccaggcc
      961 ctcgaaaagg tcgaggatga tgcctccaag ctgagctggg aactgttccg cgacaccgta
     1021 atcgaacagt gcgagcaagg tgtggattac atgaccatcc acgccggcgt gctgctgcgt
     1081 tacgtgccgc tgaccgccaa ccgcgtgacc ggcatcgtct cccgcggtgg ctccatcatg
     1141 gccgactggt gcctgcgcca ccaccaggag agcttcctgt acacgcactt cgacgagctg
     1201 tgcgacatct tcgccaagta cgacgtggcg ttctctctgg gcgatggcct gcgccccggc
     1261 tccctagccg acgcgaacga cgccgcgcag ctttccgagc tcatgactct gggcgagctg
     1321 accgagcgcg cctgggccaa ggacgtgcag gtgatgatcg agggcccggg ccacgtgccg
     1381 ttcgacaccg tacgcatgaa catcgagctg gagaaggccg tgtgccacaa cgccccgttc
     1441 tacaccctcg gaccgctgac caccgatacc gctccgggtt acgatcacat cacctccgct
     1501 atcggcgcca ccgagatcgg tcgttacggc accgctatgc tgtgctacgt gaccccgaag
     1561 gaacacctcg gcctgccgaa caaggatgat gtgaagcagg gcgtgattgc ctacaagatc
     1621 gcctgccacg ccgcagacat cgccaagcac cacccggacg cgatggatcg cgacaatgcg
     1681 atttccaagg ctcgcttcga gttccgttgg ctcgaccagt tcaatctgtc ctacgatccg
     1741 gacaccgcca tcgccttcca cgacgatacc ctgcccgccg agccggccaa gatggcgcac
     1801 ttctgctcga tgtgcggccc gaagttctgc tcgatggcca tctcccagaa catccgcaag
     1861 gcattcggtg gcgaggccgc tcagcagcaa atcgtgaagg aggctgctgc aggcatcgac
     1921 tccgaggcac tggctacggc caaggccaat gtcgataacg gcgtggtgtc cgccaacgtg
     1981 ctcagccccg aagaaatcct cgccggcatg gatgcgatga gcgaaaagta caccgcccag
     2041 ggcggcaagc tctactccac cgcccaggag taaacctctc tggctcccct ctctgagggg
     2101 agccaattat tatgcccggt ggtgtcttaa ttcgggacga cgctcgactc gctcgaaggc
     2161 ggtgccgaag tgtgcgccat ctggaccgag gccgtcggaa gtctccagga ccacgccttc
     2221 gttgagcgca cggcgcacga tgcccacacg gggcttcaac ggccacacat catgcggcac
     2281 tttgcgatag aaatccgtac cgacgagcaa cacgtcgccc acatcgaaca cttcctgacg
     2341 cggaaatgtg agctgtgtgg tgtgtccgtt aggcattacg ccaaaatatg cggtgagttc
     2401 cggtgatgcg caccaagacg ccgtcacggc gcagtacatc gttatcagcc atgcctcctc
     2461 ccgaacattt catgaatcaa tcgcaatcag caaaccttcc ccgtctcacc acaggagcgg
     2521 caaaacatca aggaaaaccg ctccgaaaaa catcatgggc cccacttaga atgaacacta
     2581 tggcacaaat tgcaacatct acacttcccg ctgttctcgc gatctccggc tccgactcgt
     2641 ccggcggtgc cggcatgcag gccgatctga agaccatgct ggcctgcggc gtgttcggca
     2701 tgagcgccat caccgcgctc accgcacaga acaccaccgg cgtgcgttcc attcaggaca
     2761 ccaaaccgga cattctcgcc gaccagatca acatggtgtt tgaggatatt ccgccggtcg
     2821 cggtgaaaat cggcatggtg tctgccactg acatcatcga cgtcatcgct gaacgactca
     2881 ccttccatca cgccacgaac atcgtgctcg acccggttat ggtggccact tccggtgcca
     2941 atctcatcag tgacgatgcc attgcggcct tgaccggcaa gctgttcccg atggccacgg
     3001 tcgtgactcc gaacattccc gaggccgaag tgctgaccga tacgctgatt cgcgatcagg
     3061 aggatatgga ggccgccgcc caccgcctcg tggagcgtta tggctgtgcc gcgctggtca
     3121 agggtggtca tggcaccgag gatgccaacg atgcgcttgc cgagacggat ggaaccgtga
     3181 cctggtttga tggcgcgcgt atcaataatc cgaacacgca cggcaccgga tgcacgctga
     3241 gctccgccat cgcctcgtat ctggcgcttg gcgacacatt gcccaaggcc atcaagcatg
     3301 cgaagaagta tctgaccggt gcactgaagg cccagctgaa tcttggccac ggctccggcc
     3361 cgatggatca tttctggaag tatcgctgag tcctactggc tctcctctct gaggggagcc
     3421 aagtaataaa aggagaatta tgattattga ccgtaatgcc gttactcagg aagtgccgat
     3481 cgacccggaa accggcaaac cgtacctcaa taccgtggcc gccattcagg tgtcggccaa
     3541 tggcgtgggc tccgaggttt cgccatacgt ggctcaggcc gtcgaggtga ttcgcgaatc
     3601 cggcctgccg caggaaacca acgccctgtt caccaacgtg gaaggcgatc tggacgacgt
     3661 gctcaaggtg gccggtgagg ccgccaagaa gctcgccgag cagggctacc gcacttctct
     3721 ggtgctgcac atggatatcc gccccggctt caccggccag ctgaccgaaa agcccaagct
     3781 ggtggatgag atcctcgccg acaagcaggc gaagtagcaa agcatcgctt ccaccagcaa
     3841 gggcatgctt tgttattaca cgtttcatgg ggcggtagct cacccatggg gtggtaaaac
     3901 cctcatgggg cggtagtcga aaacggctct attctgcgga aaggcataat ccgctaaccg
     3961 ccccatgggc caaataccgc cccatgagtg ttttaccgcc ccatgggaat cacggttctg
     4021 ccttctttcg acggggtaag agcgtaagtg gggcatatat aggagcgtca caacctctgt
     4081 tatcgttcac gatgtgaccg caacgcggcg tgccacaaaa tcgaacctat agttaagccc
     4141 catgtagtga gctatctcac aacgattggc tcgctacgta gaattcaaga cttggatcgc
     4201 aaacgtcgtt catcccacac aatgggaggg cgaacggaac acacagtcca atcaagagtt
     4261 cgctcgccgc aacaccattg agcagttgca cgcgggcagg tttgcaagaa gagggctcaa
     4321 atatgactgc tgctgaacag cagatttccg gcaatcccgc ggctatggaa aacgcacaag
     4381 cacgtaccac cgtgacggat caagccaagg ttcaggatat tatctcgcgc atggatcgcg
     4441 cacatgaaac cccgatgttc catcgcatcg tgacgctggt cgccgctggc atgctgatgg
     4501 acagcatcga cgtatatatt ggcagcgccg tcgcctccag cacgttggct acccgctggt
     4561 ccaccgttgc gcaaaattcc acctttatgt ccgcgggctt cctgggtctg ctggtcggtt
     4621 cgctgctggc cggcttcgtc ggtnnnnnnn nnnnnnnnnn nnnnnnnccg gcctgggcct
     4681 cggcgctgaa atcgtcaccg gcttcgccat ggtcaacgag ttcgcaccga tgaaccgccg
     4741 tggccactgg tgcgcgattg tctcgctcgt ggccaactgc ggtgtgccaa tcgctatgca
     4801 gctgtgtgca tggatcatcc cccgctggtc ctggcgtccg ctgttcgtgg ccatcggcct
     4861 ggtggccgcc atcatctggt ggctgcgccg cgacatcccc gaatccccgc gttggctcgc
     4921 cgtgcgcggc cgttacgatg aggccgacgc catcgtcaag cagcttgagg ccaacggttc
     4981 cgaaccgatc gacgcagccg ccaaggccga caccagcgac acccgcaacg ccggaggccg
     5041 ttcgctcggc atctgcctgc ttgtggctgt ggttgctgtg gccgccacga acgtgtgctc
     5101 ctacgccttc acttcttggg ttccgacgat tctggtcaag cgtggtatta acctgtccag
     5161 ctcgctgctc acctccacgg tgatgatgct cggtgccccg gtcggctgcc tgattggctc
     5221 tctgcttatc gaccgtatcg gccgcaagcg cacgattgtg ccggccttcc tgttcactgg
     5281 cgtgttcggt ttgatgtatg ccttccagac ctctaccgtg agcgcaatta tcgttggctt
     5341 cctgctcatg atgtgcctgt acgtgcttat ggcttccgtg gtggctgtgt acgctccgga
     5401 actgttcgcc accaaggttc gcttccgttg cgtcggcttc gcgaacgccg tggccaagct
     5461 gctcaatgtg ttgatgccga tggtggtggg ctggatgctt acctcgcttg gtgccacgtc
     5521 catctttgtg gccatcagcg cgattgctat tgcatcgatg ctgattgtcg gcttctttgg
     5581 cgcggagact gcgcagaagt cggttgggtg atcggtttcc tcacagtgga ataactgctg
     5641 ataatgtcca ttcggcgtct tgcgtggtga tatgcagggc gccgttttgt tgttctagta
     5701 attgttggta acggttgagg ccggtgccgg tggataggtc tgcgtctcca gcggacaatc
     5761 agcgtctgtg atgtcacatg caatgccagg agcggcatca acggtcgaat catgcggagt
     5821 cactgcactg tgcgccgctt ctcccctgcc ttcaatcaca tcaatgactt gatgcgtgcg
     5881 atccaatgcg cccatagaca tatccgtaac cagcacgtca ggccgcttgg tggggctcag
     5941 gcatcggctg atggccacag cacccaaatc gcaggtctat atcacggcaa aatcgctgga
     6001 catacgttcg attagcgcgc cgaacacttt cagcgcatat gggtcattgt ccacaatgcc
     6061 gatgcgtatg ttgcggttgc gcgcttcttc cattacattc accccttcag tgtgcccgac
     6121 gccgaactac tccgtcctga cacgtcatgg tcactatgac aaccatcatt ccacatttcc
     6181 tgcgtgattt acgatggaat catcatcaat ccttgacaag gcggtaaagg aggacggcaa
     6241 tgaaccgtgc acaagcactc agcaagctca acaggagcga tatgcgcaat gccgccgccg
     6301 agcgcgggat gcaacgactg ttcacatgga accgcgcgca ttggattggc atggttgtac
     6361 tgatagcggt gttgctgcta ctgcagtgga gctcatcaat gtatctcatg cccgacgagg
     6421 ttcttgacag ggctgccaaa aacctgttcg tatggttccc cggtatggtt ctgcactggc
     6481 cattggtgtt gggaatcacc aacttgatac tcggttcgac acgatccggc agtctgtctt
     6541 tcccgttggc gagcgcaatc atcggacccc tggcattcgg ctggaccaat cttcgcttac
     6601 ggccggggtt cccataccgt tcctgggctc aagggaaagc cgcaatataa tgaaaacagc
     6661 gatttcatgc aatgatgcac gagccgatcc aagcaaaggg gccaaccatg agcgagcaac
     6721 tgatggaaca gtaccggttg cgcggacaac gcaaatgccg taacgcttgt atcgccgcca
     6781 tcgtgacagt agtgcttgtc cttgccgtcg ccggcggcgt atggtggacg gccggcgatg
     6841 gcagcgcatt ggttcgcaat atgttcaagc cgaaggccac gcctgccacg cagccggtag
     6901 tcaacagcac cgcaaccttc gcctaccgca ccgcaccgga attcctggcg atggaagccg
     6961 gcgaccgagg caccggcaat gtgaactact ctcctgcttc gatgtggatg gcgttggcca
     7021 tcgccgcgca gggcgccaat ggcacgaccc gctcgcaact gaacgaactg ctgggctccg
     7081 gttcgctgac cgatagcgac taccaatcgc tgctaagttc gatcaacggg caatattcgg
     7141 gggcgaaatc cgagatgagc gccgcgaact cgctgtggat tgatgacgac tactctcttg
     7201 ccagcgatta ccaatcaacc gtcaagaaga
//
LOCUS       DAWBMW010000075         7171 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_2414_length_7171_cov_2.511804, whole genome shotgun sequence.
ACCESSION   DAWBMW010000075 DAWBMW010000000
VERSION     DAWBMW010000075.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 7171)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7171)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7171
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2414_length_7171_cov_2.511804"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3820..3829
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5338..5401
                     /estimated_length=64
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6067..6076
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1490 a         2037 c         2130 g         1430 t
ORIGIN      
        1 cacgtagcag cgggtggtgg cacgcgcgag tacggagaaa tcatgggtga tgaacagcat
       61 ggatgcgccg gcgtcgtcca ccaacgaggt cagcaggtcc acgatctgac gttgcgtgat
      121 ggagtcgagc gcggtggtgg gttcgtccgc gatgatgaat cgcggtgagg tgatcagcgc
      181 cgtggcgatg cccacgcgct gctgttggcc gccggacagt tcgtgcgggt atgtgccggc
      241 cacgtcacgg tccagtccga ccttgtccag catggtattc actcgctcag cacgttcggc
      301 cttggtcaga tcgtagtgca gtctcaatgg caacgcaatc tgctgcgcca cggtcatcac
      361 cgggttcagt gaggcagccg gattctggaa caccgcaccc acataccggc cgcgcaggtc
      421 ggcaagcgca cgttcggaag ctccgataat ttccgtgccg cccatatcaa tcgacccatt
      481 agtcacagcg gtcaggggca gcaaccccag catcgccttg gcaatcatag attttccgga
      541 gcctgaagaa ccgatcaagc cgactcgttc accgtcggca atggtcatat ccgcatcaga
      601 gacgattggt ttgccgccaa tcgtgatttt cagcccgcga acatcgacgc tcatcgttcg
      661 cctcctattt cacgtaatgc ggggttggtg accggatcga tggcatcgcg cagcacatca
      721 ccaaaaatat tcagcgccac aaccacaacc gtgacgatca ggccgggcca caccacggtt
      781 aatgggaaca cgttgatgag tttgaccgaa gtggccagag aatgccccca gctcggcaca
      841 ccggacggca caccgatacc gagataagtg agccccgact cagccaacac cgccgtgccc
      901 gccgacaatg acagttgcac agccaatacc ggcagaatat tcggcataac atggcttatg
      961 agcacacgcc acccgctggc accattggcc aaggccgatt ccacatatga cgagcgggac
     1021 gccagcaacg ctgcggggcg agccacacga gccagattca acccatatcc gaagccgcag
     1081 gcgatcacaa tcaccgcgat tgacgctccc aacggcacag ccagaatcaa cgcaatcaac
     1141 acggtgggaa cggaaatcaa cgcatcaact accactaccg aagaactggc gagcgccgca
     1201 ttgcgtgcca ccatggccgc gatgagcagc agcccccaag ccatagatgc cactacggtc
     1261 agcagcacga tcacaagatt ggtatgtgag ccggccagca gccagctgaa cacgtccgcg
     1321 ccggtgccgt cagtgccgag ccagtgctcg gcggacggct tggcccatac atggtagcca
     1381 tcagtggccc acagcgattg cggggtccag aacagggaaa cgatggcaat gagcaaccac
     1441 agagcgagca cgataagtgc gaatttgccc tcggcacgac gccacataga gtgcaggacg
     1501 atgacgaagg cagcccgtcg accaccagtc gtcgcacgac gaacattgct catgcggtca
     1561 cctccgtaat ggcggtagcg gttttcagac gcgggtcgag cacgcggtgg aacaaatcga
     1621 ccacgaggcc gacagtcaaa aagaacgcgg ccagcaggaa cagctcgctt tgcacggcaa
     1681 tcaggtcgcg gttgcccacg tcggtcacaa gaccgttgcc gatgcccggt aacgcaaaca
     1741 ggttttcgat gaccatcacg ccagtgacca tcgaagcgaa ggtcagcccg ataacagaca
     1801 ccagctgcgg ggttgccaac cgcaatccca cacgcaacac cgcctgagtg cgcgtcatgc
     1861 cacacgagcg tgccatatcg atgtagccgg aactggctaa atcccctaag gccgcgcgcg
     1921 tgtatcgcat aagcgatgct cccacgatga tgccgaccga tacagccggc aataccaacg
     1981 ctgatatcgc cgaaccgggt gcgccccagc catccagcgg gaacccttgc gagggaaaca
     2041 cgtcaagcaa tcctacgccg cgcccgaaca acagaatcag cagcagaccg ccccacaacg
     2101 caggtacggc accgccgaca atagccagta catgaaacac cgcacgcacc gttgcgctgc
     2161 gggccagtac cgccgcacag cccagcggca agccgatggc caaggcgatg agcaggccaa
     2221 gaataatcag gggaaaggtg atggacgcac gtgcgcctac caaggaggtt acggagcgac
     2281 cagtcaggat tgaagtgccg aagtcaccac gggccagcgc actcatccaa tcgaagtatt
     2341 gtgcaatcaa tggccgattc agaccgagtt gttcgcgtag ctgggtcaca cgctcaggcg
     2401 gcgagttgac gccggccatc acggaggcca tatcgccagg cagaatgcgc aatgctgcga
     2461 aaaccaccac ggacaggccg aacaacgcca ccacgaataa cgccagccgt ttcaacacga
     2521 atcgcataat gcctacttta cttggattaa caatgagccc cctcactgag ggagctcatt
     2581 gctgtaacga actacttcgt gtaggtcacg ttgtagaggg gcagaacggt ctggttgagg
     2641 tcgaagggga atccttcaac gcccttggcc gtggcggtgg tgatgcggta gttgaacagc
     2701 cagtcggccg gcgcatcctc ggagacaagc ttggcggccg cggcgaactt ggcatcgcgc
     2761 tccttgtcgg aggtggcggc aacaccctcg ttgtacagct tggtcacatt cttgttgtcg
     2821 taaccgaaat agtatgtcgg atcggtccac gatgcgaagt cgtggctttc gttgtggtcc
     2881 accagcgaaa tatcgaaggt gtggttggtg tacacatcct gcagccacgt ggagaactcc
     2941 accacattca ccttcaggtc gatgccgagg ggcttgagct gggagcgcag ctggtctccg
     3001 agctcggtgc cgtagatgtt ggcgtaggtc agcgtaagtt gaagcggctt gtcggtggaa
     3061 tagccggctt cagccatgag cgacttggcc ttgttctgat catacgggta cagattcgtc
     3121 agatcctcgt agccggggtc gagagacggg atcgggccgc cgagcgcctt gtccgcaccg
     3181 ccgcgagagg cgatgagctc cttgtggttg atggcgtaac ggatggcctg acgaatgcgc
     3241 ttgtcagcca gtttggaacc ggaagcgttg ttgaagccga gcacgaactt gtcggtgccg
     3301 ttgccggctt tgaccgtgta cttggcggaa tcggacttga acggttcggc gaggttctcg
     3361 gtaatcgggg cgagcacctg cacgtcaccg gacttcagcg cattgaccgc ggcgttgtcg
     3421 tcggccagat acttgacgac cacggtgggc gtcttggcct tgttcttgcc ccagtacttc
     3481 tcattggcct tcagcgtgat cgagctgttt tccacgaact tggcaacggt gtaggggccg
     3541 gagccgacgg cctgcgtctt caggtcatac ttggcatcct tgtcgaacac gagccctgcg
     3601 cgaccggtca gggtccacaa caggttggag ttcggcgtct tgagcgtgat caccgcggtg
     3661 tcggcgtcct tggcctccac cttgctgacg gcggcgagcg cgtcggcgtc gtggtagccc
     3721 ttagcgatca gctcgttgat ggaccaggcc acgtcctcgg cgtcaagttt gtcgccattg
     3781 gagaaggtca tgttcttatt gaggtggaag gtgtaggtcn nnnnnnnnnc tcgccgtcgc
     3841 tccagtagtt ttcacggaga tggaaggtgt aggtcaggcc gtccttggac gtctcccagc
     3901 tggaggcgat ggcgggagcg acctggttct tggagtcacg tgccacgatg ccttcgtata
     3961 cgttgccgat caggatctgg tcaagcgcgg agccggcggt gttgcggatg tccagattgg
     4021 tgggggccag tttgaggccg atggtcacgg tgtcggcata ttggttgctg gacgaggccg
     4081 acttgtcatc tttcttaccg ccgaaagcga gcgtgacgcc gatgacgacc gccactgcca
     4141 cgatgacggc aatcagtgag atcagccacc acttcgggcc gttttttttc ggattctttg
     4201 cacgcgaggc aaggctcgca tctgtggttg cgccgtcttc gatgtgcgca ccattgtctt
     4261 gagacatggt tcttcctctg tatatgttgt gttctacggt tggccgccac gctgggtcga
     4321 ccaccgcagg ccatcatagc catgggactg tagaattgcg catgaaatcc atcttgaatc
     4381 tggagggtta tcatgctggt cgccgttgat atcggcaata cgaacatcgt gctgggcttt
     4441 ttggatggcg ataccatcgc cggcacctac cgcatcacca ccaaagccaa ccatacgtct
     4501 gatgagtatg ggctgtttct gactgaattc ctgagaatga gcggttttca gccatccgat
     4561 gtggacgatg tgatcatctg ttcggtggtg ccgaaagtga tgcactcctt ccgttcgtcc
     4621 atcgtcaaat tcctcgacat tgatccgatg gtggtgggac cgggcatcaa gaccggtatg
     4681 aacgtgcgcg ttgacgatcc gaaatcgctg ggtgcggata ttctggccga ctgcgccggt
     4741 gcctattacg agtatggcgg cccagtgctg gtggctgatt tcggcaccgc caccacgttc
     4801 acgcacgtga gcgacaaggg cgtggtcgat tccggcgtca tcaccaccgg catccgtgcc
     4861 ggtgcggccg ctctgtgggg agatacggcc cagctgccgg aagtggaaat tacgcgaccg
     4921 gatacgattc tgggcaccaa caccaagacc tgcatgcagg ccggtttgta ttacacgttc
     4981 ttgggcggtg tggagcgcac gattcgtcag ttccgcagag agctcggagg cgaggatttc
     5041 aaggtgatca ccaccggtgg cctcggtcga gtgtttgaga atgacaccga gctgattgac
     5101 gtgtatgacc cggatttgat tttcaagggc atggcgcaca tctactcgcg caatgtgaag
     5161 tgacgtagta tgcaggaatc ggccgcgtat agcgcggtcg attccgatcc aaccccgaat
     5221 ccctacttaa tcgtaaagcc ctgattgagg ccgtattctc ggaggtcttt ctgccattgg
     5281 gccaggccgt cggagagact gactctcttg ccgggcttct ccaatggttc gagcgggnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nttccttgtc cttcatgccc atctcaatgg cttcctgtcg ctgctgatag gcgtggaaac
     5461 tgccggacca ctcgtatgcc tggcccaccg tcgatttgaa gtcactgcgc gcatacactt
     5521 cgaatggcag atactggtat ccgaccgaca catcctcggc ggcctccgac agtacacggt
     5581 tgaacttctg cccgccgaaa tacgggattt caatgccccg gtcattggta atcgtggttt
     5641 tatccaaaaa atcagcggaa ttcagggtgg cataatcggc cgggaaagca ccgccatcca
     5701 cacgcgcgct aatgccttgg gcgtcatgcg ccacaaagtc cacgaaacga taagccgcat
     5761 ccggcttacg ggtcaattgc agtacggtca gtgccgaccc gccgctttcg gcgttggtgg
     5821 gctggccatc ataggtgggc atgggagcca cgcgccacag accggccgcg cccggcacat
     5881 tggacagcag cagcgacggc atccaggctc cggagaatac ggaggcgatg gtaccggtgc
     5941 ccacacggcg cttccatcgg tcggaccatg tggcggaggt ggtgtcaaca agcccctcat
     6001 cgatcatttt ctgccagaat tcggtgaacc gttgggtgcc ggcgtcctca tccaaatcga
     6061 tggtcannnn nnnnnngctg gccgccggcg agccagacca tggcatcgta aaagctgcca
     6121 tcgcccgagt cggcggcgat gtacacgcca atctccttga gctttttggc agcctcatag
     6181 tagtcatccc aagtcttgat cttggtggcg tccacgccgg cttggcggaa cacgtcttcg
     6241 ttgtagaaga agcccatcgg gcccgaatcc atgggcagcc cgtaggtacg gccggcgagc
     6301 tgcacggacg accatgtgcc gagggtaaag aagttggaat aatccgagcc gattttgtcg
     6361 gtcagatcaa gcaattggcc ggagaccgcg tactgcaaca gtgcatagta ttcgatttga
     6421 gccacgtccg gcgtgccgta accgtcctga atggcggtgt tgagattgcc gtagccggag
     6481 atgttggtcc atttcacgcg gatgtccgga ttggcttttt cgaaacggcg gatgacctcg
     6541 cccatgctcg gttcccagct ccatacgctg atttccgtgc ggttatcggt ggtggactgc
     6601 ccgcacgagg ccaaggatac ggaaaggatg gcagtcactg ctatggttgc cattcttttg
     6661 attcgctgtc cgatcactcg catggtgacg agtgtactag gcggggctta aaacggtaaa
     6721 agcccggtga cgggattcgt cacggggctt tgtgtatcag gtgagattag ctaggcagtc
     6781 tcagcgaggg ttcaaggctt acttcacggt gaagccctga tccttgccgt aatcctgcag
     6841 tgccttctgc caagcggcga taccgtcagt cagcttctgg tcgccggtgt aggacttgcc
     6901 aaggtagtcg ccgaagatgg tgcgggcctt gacctcgtag ggcaggaact ggtagtcgga
     6961 ggacacgttc tcagcagcct gagccagaac ctcgttgtac ttctgaccac cgaagtagtc
     7021 aacgtcctcg ccatcggcgt tcttcacagt ggtggtgttc ttgaaggagt cggactccat
     7081 ggaggccttg tcagccggga atgcaccgcc ggccacacgg gtggccacgc cgtcgccgtg
     7141 gttggcgtac tcgatgaact ggtaagcagc g
//
LOCUS       DAWBMW010000076         7143 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_2428_length_7143_cov_2.380926, whole genome shotgun sequence.
ACCESSION   DAWBMW010000076 DAWBMW010000000
VERSION     DAWBMW010000076.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 7143)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7143)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7143
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2428_length_7143_cov_2.380926"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1510 a         2224 c         2157 g         1252 t
ORIGIN      
        1 atggaggcca gctacccgga gctcaacgac accttccatg aggtgtccga atccgcgtat
       61 ggcgaggaag acgccttccg ccgcaccctt gagaccggca ccacgattct cgacgtggcc
      121 gtcaacaagg ccaagagcga ttccgccgag cccgtcgtgg ccggcgagga cgccttcaag
      181 ctgcacgaca cctacggctt cccgattgag ctgaccctcg agatggccgc cgaacagggc
      241 gtgaaggtgg acgaggccaa gttccgcgaa ctcatggccg agcagaagtc ccgcgcccgc
      301 gccgacgccc tcaagaagcg tcacaacgtg gatctttccg tttacgacga cttcaagaag
      361 acgctcgtca gcccgatcga cttcctcggc tacaccgaca tgtccgcccg cgccaaggtg
      421 atcggcatca tgcaggaagg caagggttcc gtgcccgccg tgaccggccc ggccaacgtc
      481 gaggtcatcc tcgaccgcac cccgttctac gcggaggccg gcggccagct cgccgaccag
      541 ggcgagatcc tgtccgacga cggcgccgtg ctcgaagtcg acgatgtgca gaagcccatc
      601 aaggacctca tcgtccacca gtgccgtctg accgaaggca cgctggtcgt cggcgccgaa
      661 gtcaacgcca acatcgacct ggcccgccgt ggcgccatcg cccgctcgca caccgccacg
      721 cacatggtgc acaaggcgct gcgtgaggag ctgggcccgc aggccaccca gcgcggctcc
      781 gaagacgccc cgaaccgtct gcgcttcgac ttccagtggt ccaaggcccc ggccaagtcc
      841 gtgatctccg cagtcgagga gcgcgtcaac gacaagctgc gcgacaacct cgccgtgacc
      901 accaaggaaa tgaagttcga cgacgccatc gccctcggtg ccatgcacct gttcggcgag
      961 aagtacggcg acatcgtacg cgtggtctcc atcggcgaag acggctggag ccgcgagctg
     1021 tgtggtggta cccacgtgga ccacgttggc aagatcggca tggtcaacat cctgtccgaa
     1081 gcctccatcg gctccggcgt gcgtcgtgtc gacgccgtcg tgggccaggg tgcctatgac
     1141 ttcaacgctc gtgagcacgc gctggtctcc cagctgtccg acaagctcaa cgctcgtccg
     1201 gacgagctcg ccgagcgtgt gaacgccctg ctcgccaagc tcaaggaatc cgatcgtcgc
     1261 ttggcctcca tgtacgagtc ccagctggcc gcctcggttc cggcgctggt tgctgacacc
     1321 aagaactccg cggccccggt caaggtggcc gtcaagaacg tgggccactt tggcgcggtc
     1381 gatgccctgc gcaagaccgt actcgacgtg cgcgcccagc tcggtgaaga cgcccccgtg
     1441 gtggtggccc ttgccggcgt gaacgaggat gacaagccga tggtcgccgt ggccaccaac
     1501 gaggccgccc gcaaggccgg catcaaggcc ggtgatctgg tgcgtggcgc agccaaggtc
     1561 ctcggcggcg gtggcggcgg caagccggac ttcgcccagg gtggcggtgt cgatgcctcc
     1621 aagatcgacg aggccctcga agcgctcaag cacgaggcgc agaaagcctg attccgccag
     1681 tggtgtggtt aggcgttgat ttgggtaatg cccgggtcgg actggcactg tccgacccgg
     1741 agcttacctt tgcacatcct gctggcaaca ttcatgttgc cggcgactat ttctttgcta
     1801 ttgacgaggt cctgaacgtc atcgaagacg aacatgtgga ccatgtaatc gtcggactgc
     1861 ccctgcagat ggacggtaca gaaggcaaaa gcgccaaaaa agcgcgcagg tgggcggcca
     1921 atctggagaa gaggctgcaa gcagagagtg aagacagtga ttctacggag tatcagattc
     1981 cgcaggtatc attgatagat gagagattga caacagtcag tgcgcatcgt cagctgttcg
     2041 aggcccataa agcttcgaac aagcacaggc cggtggttga ccagcagtcg gcagtggtga
     2101 ttttgcagac ggcactcgac cgtacccggg agcagtgagg tatcggttat gaccgatgat
     2161 tttaacgagt tctttgatga caacacgcat tgggtcgaac aggggtcaga accaatgcgc
     2221 ggggccgagc cgccagaacc gccgaaatcc cgcagggaga tgcgcaagcg acgtgcacag
     2281 cgcaggcgca agcgtatcag tggcattatc atcgctatcg ccatcgtcgc cctgcttatc
     2341 ggcggcggct atttcggcgt aaccaagctc atcgcatggc gcgacagccg gagcaaccag
     2401 tcgacggcca tcgcggacta tccgggaccg ggctatggcg aggtcgaatt caccgtgagc
     2461 gagggccaag gcgcggcgga gatcgccgat aatctgctca aggccgaagt catcaagtct
     2521 actgccgcgt tcaccagcgt ggtcaacgcc aatagcacga cactctatcc gggaacgttc
     2581 acattgaaga agcatatggc cgcatccaaa gtgatcgacg ttctctccga caccaaccag
     2641 gcggcgggct tcctcgaggt tcgtcccggt gaacgtgcca ccgatgtgtt cgccaacgcc
     2701 gcgaagcttt ccggcattgc ccaaagcgaa ttcgacacga ttatcaagaa caagggtaag
     2761 gatattctgc ccaatgaagc gggcggtagc tttgagggat ggctggagcc gggaacctac
     2821 aacgtcaaga gcatgaagag cgcttcggaa atcctcaagg ccatggtcga taagcgtatc
     2881 gccaagttgg atgaattggg cgtgccggcc ggcagtgacc gcgaacgggt gatgatcatc
     2941 gcctctatcg ccgaggccga ggtcaacaaa gccgattact acggcaaagt gacgcgagtc
     3001 atcgagaacc gtttggagca gggcatgtcg ttgggcatgg attccaccgt ggcctatggc
     3061 aataacgtca agcccgcgca ggtgaccacc gagatgaccc aagacgagtc caacccgtac
     3121 aacacgtaca agatctccgg cctgccgcct acgccgatca gcaatcccgg cgataacgcc
     3181 attcaagcgg cactgcaccc cgtaggcggc aattggctgt acttctgcac ggtcaatctg
     3241 gacaccggcg aaaccaagtt cgccgcaacc gccgatgaac atgaccagaa cgtggccgaa
     3301 ctacgcaagt ggcaagccga aaaccagtag tcatcccgtt agcttccttc gcttggggaa
     3361 gctgatcgga cgttggtctc ttcattggct ccctcgccga ggggagcact gcagggaatg
     3421 taacgctcaa cttaagcccg gggctcccgc gaagcggatg gaagaaatct aggacaacac
     3481 cccaacagcc accgccacaa tcgcgcccgc caatatcacc ggcacatacg gcaccttgcc
     3541 cgcatgtgcc ggtgatgtgc gttgtgggtc gaatcgcgtc cacaaggcaa tccatgtgat
     3601 gccggcaacg cccatcgcca gccaccagac caccacaggc aacaggccaa acagtccgac
     3661 agacaatccc ataggtatca aggcggtcac atcgccgaat cccaatgacc gaggcaccag
     3721 caacgccaac gcgaactgga tcaggcaaca tagcacggtg aacaacagcg cctgcaacag
     3781 cacgaacagg tcgttcaccc gccgatgacg aacatcctca atggacagtg caagcccaca
     3841 tagcagaccg ggtagcgtga acaggtatgg catggcttaa cattaacccc tagctagacg
     3901 tacacataga cgtacacaca tgaaacgagg gaagttatgc tgcgttggca gacggcaggg
     3961 gagtctcacg gagaagcatt ggtggccatg attgaagggt tgccggccgg cgtgcgtatt
     4021 agcacggacg atatcgtttc cgcactggca cgccgtcgac taggtccgtc tgctgaccgg
     4081 tgtacgccac ggcctgaccc tcggctcgcc ggtggccatc gaaatcgcca acaccgaatg
     4141 gccgaagtgg accgaggtca tgagcgccga tgcactcgac catgacctgc cgcgcgaagg
     4201 ccgcaatgcg ccgctgagcc gcccgcgccc gggccacgcc gatctgaccg gcatgcgcaa
     4261 gtatggtttt gacgacgccc gcccggtact ggagcgctcc agcgcccgtg agaccgcctc
     4321 ccgtgtggct ttgggcgagg tggccaagca gttcttggat caggcgttcg gcatccgcac
     4381 cgtagcccac gtggtggcgc ttggtggcgt gcagaccaac cccgacctgc ctctgcccac
     4441 ccccgacgat ctggaagcgc ttgatgcctc gcctgtccgt acgctggaca aagaggccga
     4501 agcccgcatc atcgaacgca tcaacgaggc caagaaggcc tccgatacgt tgggcggcgt
     4561 gattgaagtg ctggcttacg gtgtgcctgc cggcatcggc acgtatgtgg agtccgaccg
     4621 ccgactggac gcggctctgg cctccgcgat catgggcatt caggcgttca agggtgtgga
     4681 gatcggcgac ggcttcctgg aagcctcccg ccccggttcg caggcgcacg atgagatcgt
     4741 ggtcaacgcc gacggccgta tcgaccgtct gtccaaccgc gcaggcggca ttgaaggcgg
     4801 tatgtccaat ggccaggtga ttcgcgtacg cggtgccatg aagccgattc cgtccatccc
     4861 caaggccctg cgtacggtcg acgtgctgac cggcgaatcc gcacaagcca tcaaccagcg
     4921 ttccgacagc accgccgtgc ctgcggcatc cgtggtggcc gaggccatgg tgcgtctgac
     4981 tttggcgaag tacgcgctgg ataagttcgg tggagacagt gttgccgaaa cacggcgcaa
     5041 ccttgagtcg tatctcgcct cgtggccgga gcacatgcgc tgaacgaagg gcgtatggtt
     5101 tcgtgacgac tgccgcggaa ccatcagcaa ctgtactcac caatcgtcga gaggattcgt
     5161 ggattatgac cgcacgcccc cgcgccgtca tcatcggcat gatgggagcc ggcaaaacac
     5221 gtgtcggtaa ggaagtggcg catatgttgc gtctgccgtt cgccgatgcc gatgtggaga
     5281 tcgaacgtga agtgggcatg aagattccct cctacttcga ggaatacggt gagccggcat
     5341 tccgcgaggt cgaggccgat ctcatcgccg atatgcttga ggatttcgac ggtatctttt
     5401 cgctgggcgg tggcgctccc atgacgccat ccacccagca tgccctcgcc tcctacatcg
     5461 accatggcgg tcgtgtggtc taccttgatg ccgatccggc ggaagccatg gaacgcgcga
     5521 accgtggggg aggccggccc atgctcaatg gcaacgccaa ctcccgttgg aaaaaactgt
     5581 tcaagcagcg tgacccagta ttccgcgagg tcgccaacgt tcatgtccat acccgggggc
     5641 ttacgcctca gggcgcagcg aagaaagtga ttgatatggt atctgagcga gcagtgcatg
     5701 ttaccggagc ggcaattgag ccgtatgatg tggtcattgg cgaaggtgcc atgaaccatc
     5761 tggtcgacgt gctgggaccc aaacccgcca aaatcgcgct tatccacact cagccggtgc
     5821 agcgacactc cgatcgtgcc cgcgccctgt tgcgtcaggg cggctatgag gtttccgaca
     5881 tcgtgattcc ggatgccgaa cccggcaaga ccattaccgt ggccaacggc atttgggaac
     5941 gccttggcaa cgaaggtttc acccgatccg atgccgtggt ggggcttggc ggcggtgccg
     6001 caaccgattt ggccggtttc gtggctgcca cgtggatgcg cggcgtgcgc tatgtaaact
     6061 gcccgacctc gctgctggcc atggtcgatg cctccaccgg tggcaaaacc ggcatcaaca
     6121 ccccgcaggg caagaacctc gtcggctcgt tctacacgcc tgccggtgtg ctggccgaca
     6181 ccaagacgct ggctacgctg cccaatgaca tcttcatcga gggtctcggc gaagtcgcca
     6241 aatccggttt catccgcgac cccgaaatcc tgcatatcct cgaagaccac gcagctgaac
     6301 tgcgcgcttt cgacggctct acattcctcg gctcgccgct cgaagacgtg gttgccgagc
     6361 tcattgagcg cacggtaaag gtcaaggcct accacgtctc ctccgatttg aaggagaagg
     6421 gtctgcgtga attcctcaac tacgggcaca cgatgggcca tgccatcgaa aagctcgagc
     6481 atttccgctg gaggcatggc aatgcggtgg ccgtcggtat ggtgtatgcc gccgagctgg
     6541 cccatctgat cggttacatc gatcaggatc tggttgacta tcaccgctcg ttgcttgctt
     6601 cgatgggtct gccgacgtcg tggaatggcg gctcgttcga cgacgtgctc gcattgatgc
     6661 atcgcgacaa gaaggcccgc ggcaatgagc tgcgcttcgt ggtcctcgac gagattggtc
     6721 acgtcgtacc cctcgacaac ccgcccgccg aagccgtgga agaagcattc cgtcgtatcc
     6781 agcagtaatt gaacggccgt aggccgtgcc gttcgccatc ccctcagaaa aaagtacttg
     6841 gctcccctct aacggaggta ccccggagtc ggatttacct tctacctccc tctgatgagg
     6901 gaggtgcccc gaaggggcgg agggagagaa gcacaaacaa aaggagtccc aatgaccaaa
     6961 gtcatcgttg tcaatggccc taacctcgga cgtctcggcg tccgccagcc cgacgtctac
     7021 ggtcgtcaag acctcgacac cctgcgcaag ctctgcaccg aatggggcaa ggacctcggc
     7081 ctcgaagtcg aggtgcgcca gaccgacgac gaagccgaaa tggtgcactg gatgcaccaa
     7141 gcc
//
LOCUS       DAWBMW010000077         7016 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_2475_length_7016_cov_2.373366, whole genome shotgun sequence.
ACCESSION   DAWBMW010000077 DAWBMW010000000
VERSION     DAWBMW010000077.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 7016)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7016)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7016
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2475_length_7016_cov_2.373366"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    932..941
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6436..6445
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1392 a         1909 c         2229 g         1466 t
ORIGIN      
        1 ggagaccaca tccttgttcg tgatgtagcc ttgcatttcc atgcagatgt cgatgatcgg
       61 ctcggtgtcc gtatcttcgg cggtgaaaat gaagttcgcg atacggttct cggcagcctc
      121 gacgatttcc ttacgacgat cgtaggcctt gttgccggcc gatccggtca ccagcgtgat
      181 acgcgggttt tcctcgcccc aacgctcgta gacgaagtca agcagcgcgg tgacggatgc
      241 gtagttgtgc gcataatcga cgatggcgag cgtgtttgac tgcgggtcgc ggaactgttc
      301 catacggccg gcgatacgta cggattcgat ggcgtgcagt gcgtcggcgt catcgagcga
      361 tacgccaacc gcatgggcga tggcgatggc tgccgccgca ttgaggtagt tgaagtcgcc
      421 atcgatggac agatggtagt cgccaagggc ctgatctccg tcggcgatgt ggaaggaggc
      481 gtgcgccggg tcggcgggcc aggcgacgac gtccgcactg gtgccggcat tatccgcgtc
      541 atgcagggcg aaggtggtca cgccgatgcc ggctgcctcc gcgtcctccc gcaacagatc
      601 ggcgtgcaga ctgtccgcgc cgagtaccag cgacttcgcg ttgacgatga tctggcgctt
      661 gcaatacagg taatcctcga acgtggggtg ttcgatcggg ctgatgtggt cgggggagat
      721 gttgaggaac gcggccacat cgaaagtcag gccatagacg cggtccacct tgtacgcctg
      781 ggaggagacc tccatcacca gatacttcat accgttatcg gccgcctcgc gcatcatgcg
      841 gaaggcgtcc atcgattcgg gtgtggtcag atcggattcg acgtaggtgt ggccgtcaag
      901 gcaattatcg acggaggaga acaatgcggc cnnnnnnnnn ntctgcggat atccgtagaa
      961 cgcggcgtac agcaggctca tcgccttacg tgcgtcgttg acgatcaggc cgggggtggc
     1021 ggttgcggct gagtactcgg tttcggccac gtatgcggcc aggccggctt cgtcgatgcc
     1081 gttcagatat tcggccttga actggccctt gcagcacagc agggtgcccg gcgtgacctt
     1141 gcgggtgtcg taggtgatgc cggcgaacgg ctcatccgcc gaagcgatac gggccgggtc
     1201 gtcggtccag acatcccctt ggatgatttc acgcagcaga tggtgttcct tgagcaggtc
     1261 agcggccgaa gccaaagtca aagccataat tgttactccc tattatctag ccgatacgcc
     1321 attctacagg tggggtgcag cgtgttgtgg ctcacacgtc atcgaccacg atatgggcac
     1381 atgatttgat tagaatggtg aaacgtgacg acaagcgaag caactatgca atccgggcag
     1441 tcagccggat cgggcgagtc catggcagcc aagcgcgcca gattcgagca gctcgccatg
     1501 cccgccgtca atgcactgta ccggcaggcg atgaggctga ccaacgatcc cgatgacgcg
     1561 caggatctgg tccaggacac cttcgaacgc gggttcaagg ccttcgactc attccagccg
     1621 ggctccaact tcgaggcgtg gatgaccacc atcgagcgca atgcctactt caaccagtat
     1681 gccaaggcca agcggcgccc gcagcgtgcc aacgactcca ccggtgaata cgacgactgg
     1741 gacatttacg atgccgccga acatacgtcg gacgggctca agtccgctga agaggaatat
     1801 ttggacgcct tcgcgcccga ggaaatcatg gccgcgctcg ccaaactctc gccggaacgc
     1861 cggcaggtgt tcatcgacgc cgccatcgac ggcaagtcct accagcaggt ggccgatgag
     1921 cagggcgtga aaatcggcac ggtgatgagc cgcctgaatc gcgcccgcac gcaactcaag
     1981 cgtgagctcg cctcgtatgc gaaagagcgc gggtacgtga ctggttcgtc ggctggttca
     2041 ccatccgcga agttgagcga aaacgttggt cgaatcgccg attccggcca taatgaaagt
     2101 agtggacaaa ctgttggtag caatggcgac acgcaagccg cagtgagaca tagtcatgcg
     2161 actggcgccc gcacgccaca acagtcaagc aagctttcgg gaaaggagat ctgatgaacg
     2221 atcagccggt agagcgtact cgccatatct ccataacccg tacgggggcc gatggcatgg
     2281 tcagccagac cgaagtgcat atcacggcgg tgcatcatcg ttcagtggat accggctcgg
     2341 cctctgcgtc ggtccaggcg gacgtgcata tcgttacgga gggcgactgt tttgaccccc
     2401 gtacctgctg cgacgagcgt gaacaggcgt tgattgccgc gttgcgtgcc tacttgcgcc
     2461 cggatgtggc acccgaatgt ctgatggccc ggctcaaggc cacgcttgat cactgctgcg
     2521 gcgaagataa gtaacgacat atacaacaag gcccgtctga tgtatcacca gacgggcctt
     2581 gagcgttcca taagaaacgc gaagaatcag gcgttaggac gcttgccgtg gttggcggcc
     2641 ttcttacgac gatccttgcg cttacgtccg cgcatgccca tgatggactc ctttgctgca
     2701 gtaaaaaaat aagccttcgg aattatgcca catgagcacg acgtgatgca acacgggtgt
     2761 ccatgtggcg atgagcgtca ggcttggcta ttcggttttt cagatttgtt cggcgtggaa
     2821 ggtgaccacg gtggtgtagt catcgcccgg ctcgagcaca accagatcct tgccggtgcg
     2881 gaaggcgtcg gcgtaggcgg tcatcggttc gacggccacg ccggcctgac gagccttctc
     2941 gccgatttcg ttgccggtgc acacctgcca ggagttgatg gtcttgtcgc cagtcagcgt
     3001 gaccttgatg ccgtcggggc gggtgaacac ggcggaagtg ctgccgtctt cgccgcggtt
     3061 ggtgatatcg gtccaggcat cgtcgaagcc acgtccctcc agcgtggggt tatcgcgcag
     3121 gtcgaagatg ccggaaaccg gctcttcgcc ggtgggcagc aggtgttcgt ctacggtcac
     3181 gtgggtatcg cagtgcagct caagacggca ggactcgttg tcggcggtaa tggcctgacc
     3241 ggtggcgtgc ttgccgttgg ccagccacgg gtggatgcca aatgcccacg gtgccttcac
     3301 gctgtcgttg ttgtgcacgg tgaaggtttc cgtgagaccg ttctcgtcaa gtgcataagt
     3361 ggcggtgatg gtgatatcaa acgggtagcc ggcgatgtcg ggggagcgcc aggagagcgt
     3421 cacgtgtgat tcctgcaaat caacgagctc ccacatgtag cggtaggcat agccgtgcag
     3481 ggaggattgg cggtcgaact cgtcgatcgg catctggtag tcttcgccat cgaaagaata
     3541 ctgaccattg gtcactcggt tcgggtacgg tgccagcacc cagccgttgc agcagggaat
     3601 gaccttgttc gggtcgaagg ggacgatgag gtcttcgccg cgccacttca gctcgcgcag
     3661 gctggcaccc agttcggtga cgacggcact ccattcgccg gacgagatga catattggtt
     3721 accagtgcga ggcttcaaag ccatagtgca cactcctttg tggaacaaat tgttatcgct
     3781 cacaatcgta tcgcataagt cccaagacgc ggcggcacat aaggataaaa aattggctcc
     3841 cctctggagg ggagccagcg taacagaact acttctggac tacttcgtag cggaccacag
     3901 tggtgtagtc gccgttgggg gcgagcgtca ccaagtggtc accagtgcgg aaggcattgg
     3961 ccggggcggt catcggctcg acggcgatgc catacggatg ctcggcgaac ggtgcgccag
     4021 tggcggtgta gcactgccaa gcattaatcg tctcatcgcc aatgattttc acttcgatac
     4081 cgtccgggcg ggtgaatgtg gtggtggtcg taccatcggc ggcacgctcc acgtcaaccc
     4141 aggcatcatc aaaggcgcgg ccttcgagcg tcgggccatc gttcaggtcg tagatgccgg
     4201 taaccggttc ggggccgggg ggaatcagcg cctcgttcac cgtcacatga ctggcggcct
     4261 tgatccgcag gtggcaggcg gcggagtcgg catcgcgttc ggcggcagtt gcgccctgct
     4321 tgccgttggc cagccacgga tgcaggccga gagcccacgg tgccggctca tcgccatcat
     4381 tgcgggcatt gacggtcgcg gtcatgccgt tgtcgccgag ttcgtaggtg acggtcacgg
     4441 tcacatcgaa cgggtatccg tccaggttgg ggaagcgcag cgaaagcgtc acggcgctat
     4501 cggtcaacga ttcaagcttc cacatgtagt ggtaggcgta tccgtggttc gcattgttgc
     4561 gcggtgcggg atgatattcg ttaaccggcg cgcagtaatg cttgccattg aaatcgtatt
     4621 cgccgttttc gatgcggttt ggccacgggg ccagcacata accgttacag gtgggggtca
     4681 ggtcgttgac gccgaacggg cagaaaattt ccttgccttg ataatcgaat cggcgaatct
     4741 tggcgccgag ttcgcagatg acggcggaat aatcgccata gtgaatcgaa tactgatttc
     4801 cggtgcgtgg tgttttactc gtcatggaat cactcccgta gtgaggttgg tgggccggta
     4861 gctcctgcta tcgctggccc cagtctagcg cgtctcctct atcgaggcgg ggattggcga
     4921 tatgcggcat ataataaaca attcgtacaa acacggtagc aatacgtgga gtgacgagtc
     4981 tcacaccatc atgcgagaag cacgtggatt ccttggaaac cggtgatttg tgggagacgc
     5041 ggaaaagttg tgacggcaga ttcctgtaat ccctgcctta cactggaaaa cactattgac
     5101 gttcaggagg ttccgatgac caaaagcgtt gtgctcgctg atccgcgagg cttctgcgcg
     5161 ggtgtggacc gtgcgattct taccgtacag accattctca aagccgcgga ggcttccgga
     5221 aagcggacgc gcgaggatgg gctgcccccg gtatatgtgc gccggcagat cgtgcacaac
     5281 aagcatgtgg tcgaggactt ggccgggcaa ggcgcggtgt tcgtccagga attggcggag
     5341 attccggatg ctgctgccca agcgggtatt ccggtggtgt tttccgccca tggcgtatct
     5401 ccggtagtga aggcggaagc cgaacgtcgt ggcatgcatg tggtggatgc cacctgccct
     5461 ctggtgggta aggtgcaccg tgaggtgctg cgtttcgtgc gcgaggggta tgaaatcgtc
     5521 tatatcgggc ataaaggtca cgatgaagcg gtcggtgtag tgggggagag ccccgaacac
     5581 gtgcacttga tcgagcatga atcggatgtc gattcgctgg attttgcgcc cgacaccaag
     5641 ctggtgctgc tctcgcagac cacgttgagc gtggacgaga ccgccgatac cattgctgcg
     5701 ctgaaggcca aattcccgtg gattcaagag ccgccgagtt cggatatctg ctatgccacg
     5761 tcgaatcgtc aggctgcggt caaactggtg gcccagcagt ccgattgcgt ggtgattgtc
     5821 ggatcggcca actcctcgaa ttccgtgcga ctgatggagg tggcgcagga gggcttgggg
     5881 gagcgcggca aagcgtatcg cgtggatgat gccagcgagc ttgacccggc atggttcgag
     5941 ggtctggaat ccgtaggtat ctcctcaggt gcctccgtgc cggacgaact ggtgtccggt
     6001 gtgattgacg ctctgcaaaa cttgggtttc accggtatga agtccgtcga aaccatcaaa
     6061 gaaaacatgc actttgtgct gccggcggag ttgcgcagga agaagtagtc attcttggct
     6121 cccctccgtg aggggagcca agaacggtca ccacgtgccg attggtgcgg caacggcgtt
     6181 caagaccttg agcaggtctt gcgggttcac gcccacagac aggcctcgct tgccgccgga
     6241 gaccagaatt tggtcgaatt gcagggcgct ttcatccaac acagtcttat gacgtgtctt
     6301 ctgacccaac ggtgaaatac cgccaaccac atagccggat tcgcgcattg ccaccttggg
     6361 atcggccata gaggccttct tggcacctac agccgcagcc agggccctca aatccatatg
     6421 gccactgacc ggtacnnnnn nnnnncttga acacctgatg ttcgtcgaag ccgagcttgg
     6481 tggccgcctc tacgccgtag ccgtcatcca tatggtcgtt ggagtgctcg tattcgtata
     6541 cgtggaattc cacgcccgcc ttctccagtt ggactgtcgc cggagtggaa cccgcgccct
     6601 tatcatgctt gtttttcttt gccatgatta ccgttctact acgaagagac ccccgcacaa
     6661 ggcgggggtc tcacagctta gttctcaaac gaaccgtagc ggcctagacc gcagtggttc
     6721 actcggagat ctcagcgaag tacagcaggg tacggatcat gttggaggtg aagccgtact
     6781 cgttgtcgta gaaggacacg gtgcgagcca gggtcacgcc gtcaatggtg ttgacgtcgg
     6841 tctgggtcgg gtcgaacacg ccaccatggg tgtcgccgag gatgtcggag gagacgatgc
     6901 cctcatcgtt gtagccgtag taatcggtgt cggagaaggc agccttgaag gcttcgttga
     6961 tctcgtcggc ggtggtctcg gtgttcagaa cggtggtcag ctcggtgacg gagccg
//
LOCUS       DAWBMW010000078         6995 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_2486_length_6995_cov_2.424784, whole genome shotgun sequence.
ACCESSION   DAWBMW010000078 DAWBMW010000000
VERSION     DAWBMW010000078.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 6995)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6995)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6995
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2486_length_6995_cov_2.424784"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6737..6746
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1533 a         2120 c         2045 g         1287 t
ORIGIN      
        1 gttttgatgt agtagccggt gccgtcgaat ttgaatcggt ccggatcgtc aagacgggaa
       61 ccggagttga tgcagagcat ggcgtcctgt gcctgggcat cttcggcgtg tacgtagtgg
      121 gaatcgttgg tggccagcag tggtgcgttg agtttcttgg ctatggtcag caggtcaccg
      181 gtcacctgag tttcgatggg cagaccgtga tccatgagtt cgatgaagaa gttgtctttg
      241 ccgaagatgt cctggaattc gccagcggcg cgcagggcct catcgaactg accgagacgc
      301 agacgggtct ggatgatgcc cgaggggcag ccggaggatg cgatcacgcc tttggaatag
      361 gtggcgagca cctccttatc catacgcgga taacgcatga cgcgaccttc gaggttggcc
      421 acggacgaag ctttgatgag gttgaccagg ccttcgtcgt tttcagccca catggtcaag
      481 tgggtgataa gaccaccacc ggagacgtcg tcgcggcgct gggcttcagt gccccaatgc
      541 acgcgggatt tgtcttgacg tgcggtttcc ggggtcacat aggcttcgat gccgatgatg
      601 ggcttgacgc cattggccac gcagttggtg tacatctcgt aggcgccatg catgttgcca
      661 tggtcggtga tgccgaccgc aggcatgcca agttcggccg cacgcttggc caaatcaggg
      721 atcttcgacg caccatcgag cagcgaatag tgcgtgtggt tatgcagttg cacaaagttg
      781 ttcccgtaat cagccatgcg ccccaccata ccgtgtggtc tgggcggctg ttcagcattc
      841 ccgtcggcac tccgcgatga gggcggcgca cagcatgatg acctgggcga cgagattgaa
      901 ccacaccagc gcgccgatca acgccgcgaa cggtgcgagc agcggattgc gggaggcgcc
      961 ggtcagcagt cgcgcgccga gcagctgcat caccgaaacc gtcagggaac ccgacgacgc
     1021 gccgatcagc gtgaatcgtc cggctttgat gtgcgcgacc acacgcaaca gcagcatgaa
     1081 caaggcgaag ttcagcgcga cgccggtgca gaaaccggtc agctccagca gcagaatccc
     1141 cggcagcgag ctgctggcga tgccgctcca ctgcaacacg cgccgcacca tgccaccgga
     1201 caccgtaccg gccgcggtcg aaagaatgaa cagtattgcg atggcgatgg cggcaagcgt
     1261 atcgcgtaac cttgcgacga cgatattgag ctcgtcgccg cagtcgtcga acatggaccg
     1321 tacggcgtgg cgcagcgagt tcatccagcc ggtcaccgtc caccagaaca tggccaacgt
     1381 ggccagaccg gtccatgtca atgtgcttga catggtgttt agtgcggaag acgtcagaaa
     1441 cgagtcggcc acaccgggaa tgacttcacg caccgcgtcg atcagcatct gctgccaatc
     1501 aatgttgccg accatgataa ggccgaacac gctcaccgca atccagatgc cggcaaaaaa
     1561 ggcgaatagc agaccatatg ccagaccatt cgccagcagg gggccgttcc gcgacacata
     1621 ccggtcgagc acgcgccccg gcagcgaacg tgcccacgcg tacgctcccc tgtcaatcgc
     1681 actcttcaac ccatgcatag ttcaactgta gctgttgtta ccattgtccc gaattcaatt
     1741 tgtaagtgca acgctcgtcg gggggggtga cgttttgttg gacttcgttg tctgtatggt
     1801 cgttcgcaac gatagcacgc gcgttcaggc gacgagcccg agtccgatgc gtttgtccat
     1861 gatgctcatg ccgtattcga gtttgatgcg cttgtccctg taccagacca tgcactcgtc
     1921 caacaacgtg acgaactcgt cgatcgagac accttggaag cttcgcttgt ggaagaactc
     1981 ctgcttgcct gccgaagccc ccggcggctg cgttgtccgg actgcggccc ttcgcgccca
     2041 tcgaccgcgt caggccgtgg ctcccgcaga cgcggatcca ttatgcacat ctcggtagga
     2101 acaaaacaac gtcaatgaag aaacctgaag aatcgctggt tgtcctgtca tctttagatg
     2161 acaggacaac cagcgacaag agtaatcgcc tcccgttgtc taaatgaaag tgcaacaccc
     2221 gttagattgg aaattgccta gaaaaccagt ccgaagggat gtcgcaccta tgggagaagt
     2281 gtattcgcac ctgtcggaag aggaacgcca ggtcatccag atcgaggtag gcaacggcgc
     2341 cagcatacgc gggatcggcg cgatgctggg ccgcagcccg tccagcatca gccgggagat
     2401 caagcgcaac acatggttcc cgtccaacga gaacgagtcg taccgcccgt accggccgaa
     2461 aagactgaag gcggggccat ggaccggccg ctactacatc gccgggcccg cgcagcgcaa
     2521 ggccgaccgc aggagatcca agccacgaaa gccctaccgg ctgtcgcacg accgcctgtg
     2581 ggcgcaggtg gccgaatggc tgggccgcgg ctggtccccg atccccggcc gagtgcccat
     2641 ctccgaacgc ccgccggagg ccgacggcag gagcgcgttc ggccattggg aggccgacag
     2701 cgtcatcgga gtgggatgca acctgcacac cgaggtggag aggaggacca ggttcctcat
     2761 ggcccgcgtc gttccggaca agaccgccgg cgagagcgtc ggggcgcagc tggacatgtt
     2821 ctcgccgctg ccggccggcg cgcgcgtcag cgtcacgcac gacaacggca ccgagttcgc
     2881 ccaccacgaa aggctgcgcg acgggctggg catggccacg tacttcgccg acccgtactc
     2941 cagccggcgg cggggaagca acgagaaccg caacggcatg atccgccggt atctgcccaa
     3001 acgctgcgag atacggatgg acatggcaaa ggaggtcagg gagatcgtcg acgagatcaa
     3061 caaccgcccc atgcgcgtgc tcggctaccg cacgcccgcc gaggcgttcg ccgacgaact
     3121 gttagaatta caggaccaac aagggtgttg cacttctaaa tagacatcgg ggtaggctcc
     3181 gcccgcaagt cacagtggta caattttcag gtttgagact gtagcaagtt gagtcgcttt
     3241 atcagtaagt ctgtatttgg aggtatcgat ggcccatcgt ccgcttatcg gcgtggtccc
     3301 gttgtgggac gacacactga atagcctgtg gatgctgccc ggctatttcg acggcatcat
     3361 cgaagccggc ggcatcccgg tgatgctgcc gctgaccgac gacgagacta caatcgaaca
     3421 gctggtcagc caatgcgatg ggttcctggt caccggcggc cacgacgtgg atccagaacg
     3481 gtatggcgag gcggccggcc ccaagacggt caaactatgc aaggcccgcg accggatgga
     3541 ggagcgcctg atcccctccg tcatcgcggc ggacaagcca ttgctgggca tctgccgcgg
     3601 catccagagc ctcaacgtcg ccttgggtgg cacgttgtgg caggatctgc cggacgaaca
     3661 tcccagcccg gtcgtgcatc atggcgacaa gccgccgtat gacccggtgg tccacgaagt
     3721 gtcgatcgcg cccgattcgc cgttggcccg cgcgttgtgg ccgctgggag acaacggccc
     3781 gagtgaggcg gaggccgatg aggtcgattc cttcggcagg ccgtatcatc cgcgcgccta
     3841 tacgctgggc gtcaacagtt atcaccatca ggcgattcgc acgctgggcc agggactgga
     3901 accgatggcc actgcgccgg acagcatcgt ggaggcggtc tggatgccgg ccaagcgttt
     3961 cgtctgggcc gtccaatggc atcctgagtt ctctcagcgc gccgatagca accaacgtcg
     4021 catcctcagc gccttcatcg acgcctgctg acgcaagctc ccgccaatct cgacgaaacg
     4081 tcttccaaga aatggcttgc ccgcatccgc agccccctac cacgacatga gcgcccttga
     4141 caacgtcacc ctcggcatgc gcttcacccg tgacagcaaa caggtcaggc agcgcaaggc
     4201 caaggccatg ctctcccggc tcggactcgg ccatgtcgcg ggagacaaga tcatgaccct
     4261 ctccggcggt gaacagcagc gcgtcgccct cgcacgatgc atattgaagc ccggggattt
     4321 gatccctcgc cgacgagccc accggcgcgc tcgaccgcga tctcgcccaa catgtcttcg
     4381 acgaaatgct ccgcctccag cacgaatacg gcaagaccct catcatcgtc acccacgatc
     4441 ttggcatcgc cgcacaatgc gacaacacca tcgagctaca ggcggtttga cggagatatt
     4501 ccggcagctt tggtctaccg aagtcacgtg ttgcccttca atctcctcgg aaatctaggt
     4561 ggcccatttt tcaaagaatg cgatcggacg taacgccccg taaggagacc tgcaatatga
     4621 aacgttgaat cgtcatctaa agatgacgtt ttcgtagatc ttccggaatc gccagtcatc
     4681 tgcgatccgg atatggcacc ggtccggctc gatcgagccc ggacgaccat ggagaaaacg
     4741 aacgatcgaa tccgaacgcg ggtaaagagc ctgcgcgaca ccattgataa agccaaattt
     4801 aagaaaacag atcagtatgc ataagtttta catgccttca gcctcgtcaa ccggcagcgg
     4861 gccccagaat ccagtcacag ccaggcagag cgtacaacat actgcgtagt acatggcgcg
     4921 aatcccaatg accaccatcg attgatgacg gccacgaata ctcgtactca gccttactgc
     4981 tttggctcat ttctgttcac ttcacgctgg agtaccgaga cacgttgagc ggaaataccg
     5041 gtaattcacg tacggttacc gaccattggc ctgaacggta ctcagcatcc gcagtcactt
     5101 ggggtatggt catgacggag agccatcatg accgatctct cattattctg ggatgaatcc
     5161 ggcgaagccg gaactgaatc taaatattaa aacgcatgat gcacacgttg cggaattagt
     5221 ccaccagaac ttcggcatgc tcggcaggtt cagcgggttc gatgtgacgg gcacgcatgg
     5281 catcgagggc ggcggccagg tcggccgggt actgggaccg gaccgtggtc cagacgcggg
     5341 tgcgaggatg gcggaattcg agcttcatgg cgtgcagcca ctgccggtca agtcccaaag
     5401 cttcagacag caccggattg gcaccataca tggggtcgcc gcacagagga tgcccaatcg
     5461 aggagaaatg cacgcgaatc tgatgggtac gtccggtctc cagattcacg gataccagtg
     5521 tggcctcatg gccgaaacgc tccagcacat cccagtgcgt aacggccggt ttgccagccg
     5581 gagtcacgca gaagcggaaa tcggacactt tggcgcgccc gatcggtgcc tcaatggttg
     5641 ctttgttctc tttaagacca ccctgcacga gcgcatgata ggttttcttt acctcatgct
     5701 cggcgaactg ccgacgcatc tcggtgtacg caaggtcgga cttgcacacc aacatcaagc
     5761 cggaagtgcc tacatcaagg cgagaaacaa tgccctcacg cccggccgca ccataggaag
     5821 tgatatgcac gccgcggtca cgcagtgatc cgagcacggt tggaccggtc cagccaaccg
     5881 aggcatgcgc cgccacgcct acaggcttat cgactacaac gacatcatcg tcttcgtaga
     5941 caacggccat atcagttgcg ataggctcag gctcggcctg ttcctccgcc aaatcaaatt
     6001 cgacggtctc ccccgcctgc aatgtgctgg accgcgcaac ttcacgtcct aaaacgcgcg
     6061 cctgcccgga gtcaaccaga tcggcggctt tagatcgtga aatacccagc attttggcca
     6121 cggctacgtc gaaacgctta cccaccaatg catcgggagc agggactatt cgactcatta
     6181 cttggcgtcc gcctccggct cagactgtag ggattcgtcg gattgctcga tgagatcctt
     6241 gtgtgagaac ggctccccca taagaatcag aatcaccagg accacaccgg cgactaccag
     6301 atagatatcc gccacattgc caacggacca gccatagtta aggaaatcca ccactttgcc
     6361 gtcgaggaag ccgtcagcgt acataacacg gtcaatcagg ttgcccagag cgcctgcaaa
     6421 cgcgaaagaa atcgccaccg accatttcat ggagacagtg cgcaccccag ccacagcaag
     6481 ggccacgcaa gccacaacgg ccaacagact gattacccaa gtagcgcctg atcccatgcc
     6541 caaggacgcg cccggattac gcaccaaggt gaaggacagc agtccgggaa tcacgcgaat
     6601 ggtctgaccg ttggataacg cggccatcgc ccaagccttg gtgagctgat cgacaatcag
     6661 cgcagccgcg gccacacatg caaagacggc cacgcgggtg cgcagccgtc cctgttggtt
     6721 cgtcatacga cctgtannnn nnnnnntacc tgcctacgct cagttggtct tggacgaggt
     6781 ctggtcgacg tagttgttcg actcggaaac ctgcgatgcc agctggccga ggtactcggt
     6841 gaggcgggcg cggtattcag tctcgaactg cttgaggctc tggatgttgc cctcgacgac
     6901 cttggactga gcctgaaggt tgtcgcgcac ctgctcggca tacgtatcgg ctgcagagcg
     6961 ggtgttcttg tcgtacgcat cagccgcgga acgag
//
LOCUS       DAWBMW010000079         6947 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_2502_length_6947_cov_2.978961, whole genome shotgun sequence.
ACCESSION   DAWBMW010000079 DAWBMW010000000
VERSION     DAWBMW010000079.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 6947)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6947)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6947
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2502_length_6947_cov_2.978961"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1574..1583
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5724..5733
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1431 a         2124 c         2018 g         1354 t
ORIGIN      
        1 ctccgattca gtggtttctc ttgcttttgt gtgaccgccg cactgcaccc acacttttca
       61 gcacactcgc ttggtatcca cctgttgacg gcacccgacg aatcggaaaa gatagcccag
      121 tccaccggcc cgatggcctt cgcccctccc gtcggcgcat tactgcgtgc gatcagcggt
      181 ttgaaggata tcccagcatg ctcgcctgtt acataaggcg tgctgctggc acacacgaat
      241 atctatacta cgattcctcc ggacaacgac gcgccgagcc acaactcact ggtttgggga
      301 cctcctgcgc ccccgtactt ttcctctcct cccaacgcaa tattccaatg cgtcgcaaaa
      361 cacacaccca actattctcc caacgcaata ttcccgggaa tgcgtcgcaa aaagggaacg
      421 ccacacagca ggtgcccccg cggagactat gtcacacggg ggcaccgcca aggaggttac
      481 attatgtttc ttttcaccac atccgcttct gtgttatctg tttccagcac atcctcgtgc
      541 tcttccatat ccgatgggaa cctctagcga cccggcctct ttgccgtatt accttgccgc
      601 tttctgcatt accaattata gcaaaaccgc cctcgcgaaa cgcccgatac ctcaatcggg
      661 agtaagcggg agcggctatg ttatgcgagg taggaaaggt ggatgaccga ctgactggag
      721 tcgtgcctta cccgaccttt ctcaccattt caggatttca ggcagcgacc ttggcggcct
      781 tatcggcgtc gaacgggtgg gccgctgaaa cggtgctctt ggcagagccc ttctcgccat
      841 tgtcggaatc ccagtggatc tcgtgcttgt catcgtggtt gcgccaccag ctggacagga
      901 tagagccgga gatgttgtgc cacacggaga agaaggtgga cggcacggca gcgagtgggt
      961 tggttgcgaa ggaggtcagg gccagggttg cgcccagagc ggagtcctgc atgccgactt
     1021 cgaaggtgat ggccttctgc tgtgcgtagc ggaagccctt cgggtagatc ttgtacatca
     1081 gcttggagaa gccgaagccc agcgagtagc cgcacaggtt gtgcaggatg accaccggga
     1141 tggccaccag ggagctggcc acgaacagct tcggaccgtt ggcggcaacg accacgccga
     1201 tgataagcag aatagccacc tgagaaacga taggcagggc gaccgtaacc ttctcgatct
     1261 tcttgccgaa gatcatgtgg cagatcacac cgagggcgat cgggaagagc accaccttga
     1321 cggcgttgag gaacagggac tgagtcggca cggacacgta ctgagaagcg agcagctgca
     1381 tcagcagcgg aatcatgaac ggggcgcaca gggtggaaag gatgccgatg gagacatcga
     1441 gggccacgtc accacgggac aggtagctca tcacgttgga ggaggtgcca gacgggcagc
     1501 agccgacgag aatcacgccc acggccagcg ggccggacag gtggaagatc gcgcacaggg
     1561 ccacggcgac gagnnnnnnn nnngcgatga gcggcatgat gatgaagtga gctacggtgc
     1621 cgacgatgac catcagcggc tgggtcagga tgcgcttgaa gtcgtccagc gtcagggtca
     1681 ggcccatgcc gaacagcacg atgcccagca tgtagccggt gtacgccttg ccccacaagc
     1741 tggcggccgg cacgaagtag ttgaagacgg cccagacgat gacgatgacc gtaaaccact
     1801 tggtcagcca atctgcaaag gctttaacct tttccatggt gtttctcttc ccttgtttgg
     1861 tgtaggtaac atcgcatgaa actaggcggg tcgcggttga tatgaaacat aatgctcaca
     1921 ttgcgaacac tcacgtaatc atttctgcaa tccccggtcg taccatcaca ccgtcgtgtg
     1981 ccttgacttt tcatcaatcg ccgcacaaga cccactcatc cacatcccgc catctgagac
     2041 agcccgcagc ctggccccaa gaatgaaacc gaccacttga ttccatcgcg acacaccgat
     2101 gagcacacat ccgcaaagtt gtgtatagta ttcacctgtt gcgcgaattc agcttgctga
     2161 atacggcact atggtggcca tagctcagtt ggtagagcat ctgattgtgg ttcagaaggt
     2221 cgcgcgttcg agccgcgttg gccaccccac taaaaccctt ccgttcgcgg aagggttttg
     2281 ttttttctcc ctgaattccc cgctcctggg ttataggcca aataggcatg ccgggaacca
     2341 ccgcgttgta cactcagggc atatccaatt caccgaacat aaggacaaaa ggtccgtcat
     2401 gctgtctttt gagaacgatt actcccgcgc cgcccatccc gcggtgcttg aagccgtggc
     2461 tgaagccaac aaccatctgt attcgggcta cgggtccgac gaactgagcg atcaggccaa
     2521 ggccaagatc cgcgaggcct gcggccagcc ggacgcggat gtgtggttcc tcgtcggcgg
     2581 cacgcagacc aaccaggtcg tcatcgacac catcaccccg gcgtatgcag gcgtggtcgc
     2641 ggtcgcctcc ggccatccga acgtgcacga ggccggcgcc atcgagttct ccgggcacaa
     2701 ggtgctcacg attccccagc acaacggcaa aatggacccg accgaactgg acgagttctg
     2761 caagaccttc tatgcggacg gcaactacga gcacatggtg ttcccgggct ccgtgtacgt
     2821 ctcgcaaagc accgaatatg gcaccatcta ctccaaggcc gagttgcagg ccatcgccga
     2881 tgtggcgcac aagtacgaca tgccgctgtt cgtggatggc gcgcgactcg gctacgcgct
     2941 gaccgccacc ggttcagaca tgacgctcgc ggatctggcg accatcgccg acgtgttcta
     3001 catcggcggt accaaggtgg gtgccctgtt cggcgaggcc gtggtgttta ccaagcacaa
     3061 cacgccgaag cacttcttga ccctcatcaa gcagcacggt gccttgctgg ccaagggctt
     3121 tgtgctgggc gcgcagtttg acgctttgtt cactgacaac ctgtatttca agatcgcccg
     3181 caacgccaat gaggcggccg atcgtattcg cgaggccctg cgcgccaagg gctacacgct
     3241 cactttcgaa gcgcccacca accagatttt cgtgacgatg gatcagccga ctatcgaccg
     3301 tctggagcgc gaggtcaagc tcggctttac cgagaaggcc gacgacacgc ataccgtgat
     3361 gcgcatctgc accagctggg ccaccacgca tgaagaggtc gatcagctta ttgagttgtt
     3421 gtaaaaccct tcgctaatct ctccctccgt catcgcttcg cgatgccacc tccctcatca
     3481 gagggaggtt ttttcgtagc tccctctgat gagggagctg gccgccgcag gcggactgag
     3541 ggaaagacct ctccgcaaac gagaaggctc ctactcccct atcgccttgc gttccatatc
     3601 cacgaacttg tggaccgagg cttccacgga ataatgcgcc ttggtgtatt cggcgtattt
     3661 ttcgccccat tcggcgcgct cggcctgatg tgcaatccac caatcgatgc gtcgggccag
     3721 cagggccgcg tcccgcacgg ggaacaacga ctcgtcgatc agcgcgaact ggctggccgc
     3781 cgacagttcg gacgcggcga tcaccggcac caatccgcac gccatcgcct cgatcacgct
     3841 caccgattcg atgtcggcaa tcgaacaatg cacgaacaaa tcgcccgaac gcaacaaatc
     3901 gggcatatcg gcgtgcttgt ggaatccgat atcggccttg cgcgccagca aacgcccggc
     3961 gcggaacttg agatactgct tgagcggccc agtaccggcg atcaccagct gaatatcgcc
     4021 ggcgtgccgg gacatcgaca ccgccttgat cagcgtaatc tgatctttct cgctagccag
     4081 tcggccggaa gcgatgacgc ggaacggcac gggagccgag gcatccggat cggccggctt
     4141 cttcggcgaa tacaccggtg aataaccatt ggaaatcacg tgcaacttgg ctttataccc
     4201 gtgcgcgcgc aacaggctcg cggtcatttc ggtgggcaca tggatgtggt caatgtgctt
     4261 gtacagccag tgcttgaaca gccagtacag gaagctggaa ataccgggaa tgcgacgtag
     4321 agggccggcc gaatacagca cgttctccgg ctgcaggtgg aatccggcag tcacgggaat
     4381 gcccatttgg tgggcgactt tagcggcacg acggccgaat ttgaacggca tatagatatg
     4441 caccacatcc acgccttgga aggcggtgcg gaacaacgtg tcgctgggct cggcgaattg
     4501 catgagctgc ttggcggcca cccacgagac gagcggcacc ttattcacgc gcgccggata
     4561 ttcgggcgcc cctataccga caagcctcac atgatggcct tgccgctcca attctgcagc
     4621 ccattgcaag gccgaattgg aggtgccgtt gccctgattg cccaccgtat cgaccacaag
     4681 agcgatagtc agcggacgat caatgctctc ttcaatcccg ttttcgcgca ttgcctcatc
     4741 ctaaaccacc gcgctgcaat ggcgtcggcc gcgcggcctg aacgcgtgtg aaatggtatg
     4801 ggctgccgca ttttgcccaa tattgaatca cgcgctccgc agcgcacccg gagtctccag
     4861 caggctcagc gagcgcacgc cgccgaattc gagccccgca taggcgaaat catcgcggca
     4921 tggcagaatg tgggtatgac tgagaacgcc gtaaatactg ccgtgacttc cacctcctct
     4981 cccgcaattc ccgccgagac ttccgccgtc tcccccgcaa ctcgcgcagc caaccgcccg
     5041 ctgggcacac cgctgggcaa gcaccccacc cgcgtgctgt ttttgggcgc tggtgagctg
     5101 ggcaaggaag tggccatcga gctcatgcga ctgggcgcgt gggtgtgcgc cgctgactcg
     5161 tatgccggcg ctccggccca gcaggtggcg catgaatatc gcgcgttgga tatggcgaat
     5221 gccgccgaat tgcaggcatt gttcgatgaa atcaagccgg acatcatcgt gcccgaggta
     5281 gaggccatcg ccaccgatgt gctggccggt gttgcggcag cgggcgccca ggtggtgccg
     5341 agcgccgaaa tcgccacgat ttgcatggac cgcgagcgcc tgcgcgtact ggcccacgaa
     5401 gaattgggct tgccgactac accgtatcgt tttgccggct cgctggaaga gctgcgcgcc
     5461 ggagcttcgg aagtcggcta tccgtgcgtg gtcaagccgg tgatgagctc gtctggtcac
     5521 ggccagtcgg tggtgcgttc cgccgacgcc atcgatgccg cttggaccga agcgcaggag
     5581 ggccgtcgcg ccgccgatga gggcgatgtg tcgcgcgtga tcgttgaggc gcttgccccg
     5641 ctggagcgtg agctgacggt actgactgtt tcttcgtccg ccggcattgt gacgtgcgcg
     5701 ccgatcggcc aacgtcagga gtcnnnnnnn nnnacccgat ggtgaggccg agcgggcgcg
     5761 cgacatcgca cgtaccgccg ttgaaggctt ggtggccaag gctcaggcgg ccggcgaaac
     5821 cggttggggc gtattcggcg tggaactgtt cgtgctcacc gacggctcga ttctgttcaa
     5881 cgaggtctcc ccccgtccgc acgataccgg tatggtgacg atggcctccc agcgtctgag
     5941 cgagttcgcg ctgcacgccc gcgcgattct cggcctgccg atcaccccca agcatgtggc
     6001 gctcacgatt ccggccggct cggtggcggc aagccatgcg attgtggtgg ccggcgacgg
     6061 tgaggtcgag ttcaccgatg tggccgctgc tctggccgaa cccggcacgg acctgcgcat
     6121 ttttgccaag cccgaagtgc atggccaccg ccgcatggcc gtggcactgg ccgtcggcga
     6181 gtccgaagcc gacgcccgtg ccaaggccgg cctcgtggcc gatgcgttga cgattaccgt
     6241 ggagtagtcc acggcctgat cagctcagca tggcctcaat attattgccg tccgggtcgt
     6301 ggatgaacga tcatgacgaa cctccttgta ccatttcgtc actcccatta tcgtgtgtat
     6361 atggccgaag agtcatgttt cgtctattga cgaacatctg agtattcaca cgcgcttctg
     6421 cagatacctt catgccggaa ggctacacga ccggagacga gctgatcgta ctggccaatg
     6481 acggccgcct ccgccatccc aacagcgtat ggccgttcga tgcagcgacg tatgatgcgc
     6541 cggccgcatc gtagcgtcat gctgagtgtt gttgtagccc tcgattggta cccttttacc
     6601 gttagttcac ccagaaattc cactcctgaa aggtgagtca tggagaagct ggaaaagctc
     6661 tacgagggca aggccaagca actgtacgca accgacgatc ccgaggttct gtgggtcgaa
     6721 tacaagaaca ccgccaccgc tggcgatggt gaaaagaagg aagacttcac cggcaagggc
     6781 cgtctgaaca acctcatcac caccatcatc ttcgacctgc tcaagaagcg cggcatcgac
     6841 agccacctta tcaagcgagt ggacgacacc ggccagctgg tgcgcaaggt gaacatgttc
     6901 ccgttggaga tcgtgctgcg caacgtggcc gccggccact tctgctc
//
LOCUS       DAWBMW010000080         6933 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_2508_length_6933_cov_2.153388, whole genome shotgun sequence.
ACCESSION   DAWBMW010000080 DAWBMW010000000
VERSION     DAWBMW010000080.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 6933)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6933)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6933
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2508_length_6933_cov_2.153388"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    253..262
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3722..3731
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4042..4051
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4609..4618
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1311 a         2243 c         2053 g         1286 t
ORIGIN      
        1 ggcggcatcg ctgtcctgaa gggcaacctg gcccccgacg gctgcgtggt caagaccgcc
       61 ggtgttccgc cggagatctg gaagttccgt ggaccggctc tggtggtcga ctcccaggag
      121 caggccatcg aggtcattct gaacgacacc ctgaagcccg gcatggcgct tgtgattcgt
      181 tacgaaggcc cgaagggtgg cccgggcatg caggaaatgc tttatcccac ttcgtatatc
      241 aaatcccgtc atnnnnnnnn nnagcaggtc gccatgctca ccgacggtcg ctactccggc
      301 ggttcctccg gtctggacat cggccacatg gcgcctgagg ctgccaacaa gggcccggtg
      361 gcgctcatca agaacggcga catcatcgat atcgatatcg aagcccgttc cgtgaacgtc
      421 gaactgactg acgagcagct ggacgagcgt cgtcgcgagc ttgaggctgg cgacggctac
      481 gtggctcacc gcaaccgcca cgtctcccag gcgctgaagg cgtacgccgc cttcgcccgc
      541 tcggccgaca agggtgcgac gcgtgacccg gagctgatca acaagctctc cggtctcgac
      601 tgatcgccga ttgcatagct gaaaagcccg gtatcttcct cgcgaagatg ccgggctttt
      661 tgctgtgctt ccgggcggga gcgatattgc ttgttatcgc acgcaggcgg cgggggagag
      721 gcgcgcgcct cgggcccagt cggcggcgcg catggccttc ttgccctgcg ccttgacttg
      781 ggtgagttcc agcggcgtgc tgccggtacc aacccacacg ttcttcttgc ccaccttcag
      841 ctcgccgggc tgaagctcgg ccggagtgtt gggattggac tggtcggccg gttgcgcggc
      901 gagaatatgc agggtgaggg gtttggcggc cggctcgtca ttatcggcga ttggcccctc
      961 cgcaaacagt tcggtccatg ccccaggatg tggcgtgcag gcgcgaacct gacggtcgat
     1021 ggcctcggcc tcgtccgtcc agctgatacg agcatcttcc accgtgattt tgtgcgcgta
     1081 ttccaggcct tcggccggtt gcgcggtgaa tgtggcagtg ccttcaccca cggcggccaa
     1141 ggcatccaca tacatgggtg cgccttcctc ggccagacgg tcgaggagtt cgccggaggt
     1201 ctcacggccg gtcagctcga tggtcaggga agcgacgatc gggccatcgt ccaaaccctc
     1261 gccgaccttg aacacgtcgg cgccggtggt cgggtcgccc gcccaaatcg cacgctgggc
     1321 gggagcggcg ccacgccact tcggcaagtt ggagaaatgc aggttgtacc agcccatggg
     1381 cactgcgtcg agtacgttct taggcaggat attgccatag gcgatgaccg cggcgatatc
     1441 cgcatgcagg ttgttcagcg cttccatgaa ttcgggggag cggggtttga gatcgatgac
     1501 gggcaacccg agctccagag ccgtcgcctt gaccgggctg ggcgtgagct tgcggccgcg
     1561 gccggtaggc gcgtcggggc gggtgatgac gcctaccaca tcaaaacgtg gatcggcggc
     1621 aaaggccttg agtgagggca ccgctacatc gggggtgccg gcgaacacga gtttgagcat
     1681 ggtcctcctt aaaacgtcga tgctcactgc acggccggaa tgttcacgcc attggcgatg
     1741 agcagttcac gcagcttgac cggatcctgg aaacgcacgc cacggatgcc ggccgcattc
     1801 gaaccgacga tgttcatggc cttgtcgtcg atgaacaacg tgccatcggc cttgataccg
     1861 aactgattga gcgcggcctc gtagatttcc ttgtgcggct tgcgcagctt gacgaaaccg
     1921 gagacgagct tgccatcaag ctgctgcaga atatcgcact gttcctcggc cacgtggaaa
     1981 aggctggatt cccagttgct caggccccac acgccaatac cggcggcctt caggtcattg
     2041 accagcacgc gtgcgcccgg cacgatgccg gtcagggagt cgcggaagtt cttgatgtag
     2101 tagtcgagga tgtccgccca cttatcgccc ctgtcacggc gcatgatggc gatggcctcg
     2161 tcggtgctgg tgccgccgtc catcaggtcg ttgacatcgt agaagccgga gatgtcgttg
     2221 tcgaggaatt cgtcgatggt cttctggctg tagcggggga tgagcacggc cgctggatcc
     2281 cagtaaatca gcacgttgcc gaaatcgaag atcacgtcgg tgatcggctt gccggcgttc
     2341 gcggcggctt cgccgtccgc gaccagcacg tcgtaatcca tatccggctc tccgggccaa
     2401 cccttcatgg tgtcctcctt atagcgatgt tcgttctgta atcatcgttt cacgtgttgg
     2461 agacgattag atcagatctt tgggatctac tcggaagcga agctcgccgg gctcgcggga
     2521 cgctacatga cgagcggttt cccggtgcag tcgggccgcc agttcggctc gccgactctg
     2581 tggcacacgg attactgcct tcacccggtc ggccgtggct tcgagctccc tggcattaac
     2641 cgtgtccggt tgcgcgatcg gcaccggtcc caacatgccg ggcagctcgc cgcaggccgt
     2701 ccaatcgccg cccagggcgc cgatacgctg catcagcgtc atcaccgcat cacggcggcc
     2761 ccacacgcag gcggcggcca cggccggcgg cataccggtc tccacacgct cctcgagatc
     2821 cttggctgca agaatcctcg aatcccagag catcagcgat tgggcgatgg ccgggtcggt
     2881 ctcgccgaga atcaacgcct ggccgccacg ggaacgcggc gcgcacaacg acatcgcacg
     2941 catccacgcg gtcaacgtat ccaaccgagc atccacgccc aacgcataca agctggtcca
     3001 agcgtcgagc acggccacgg cacgatactc atggccagcc gatccctgtt cggcactgac
     3061 cggacgcacg cgcggttcga aaccgggagt ggcgatgaca atcatcggct ggcaagctac
     3121 atcgcgcacc agaccctgcg tcttgctgga caaaactact ggcacgccac ggaacaatcc
     3181 ggccagctcc gccgcagtgc cggcagcccc cacacgcacc acgcgcatac gttcatgccc
     3241 acagcccggg cacttccagt tgatgacggc agccccacac cagcggcatc gcggtgtaga
     3301 atcccgacgg tcggccggca gttgcaacgg tccggaacac ttggcacaac gcacctgacg
     3361 atggcatttc gcacagctca aggtctcact gacattgtct tgcggaatgg atagcagcac
     3421 ggggccactt tccaaagcct tagatagcac gcggacggcc gtatgcggca cgcgcgcccc
     3481 gatggacggg tcagcgagac gcgcaagctc atcgcgattg agccaacgca cccaaggcgt
     3541 cgcatccttc agtggcgagg cgagcggatg aatcgcggtg gaatagccgc tcaccggagt
     3601 ttccaccgta cctggaccgg tgttttccca ctgactttgc ggagtgcgcg cattggccat
     3661 ggccacgaac acgccgccat gggacttggc gcgcaaccgc atcacgccgc gagcctgtgg
     3721 annnnnnnnn ncatacatca ccgcgcgggt gccgatcacc accttcacct ggcccaacgc
     3781 cacagccaga tacgtgcggt accggtcggc cgcaggcatg gtctccgcat tgagcacggc
     3841 gacatcgccc atccatccgc cggttggagc cggggcaaat ggtttgagtc cataattgcg
     3901 tagcatatgc atcaggtctt cgacctcgcg catggtgggc aattcaacta cggcagcctt
     3961 accggcactc aacgcagtgg ccaccatcca tgccatgttt cgttgccact cctgtgcacc
     4021 gggcaacgaa tcaaacacaa annnnnnnnn ncgatacccc tgcgcagctc cctccgaaac
     4081 ggtgctagca acggcaaagg tagagccagc aagtttggtc ccatcgggat tggtgccacg
     4141 cccggcaaaa ccgccttgcg tattatccgg caatgagccg ccgaccggcc gacggaacga
     4201 cgcggccaac cgctgctcgg tctccacctt ggccacgcgc ggtggcacgg cgaaacgcag
     4261 aatattggcc cgtgtgccgc cataggcttt ggcaaccaga ccgatatcct cgcgcatcga
     4321 agcgggaacc agtacatcgg ggcttagcac tcgctcgata tagcgaatcg aagagcgcgg
     4381 cgtatcactg gtatcggtgc gtgcccaaat cacaccgctg accctctgac cgccaaaccg
     4441 cacgcgcacc agcacacccg gttgtgcggc ctcgctgtct ttttcatcaa tgaagtaatc
     4501 gaatgtctga ccgaggtgga gcgcctgcac atccagcacg acttgcgcta tgggatgctg
     4561 ctcggccggc gtatgttccg caggcgcacg tttacgacgc ttgcggggnn nnnnnnnngc
     4621 cccgcaagcg tcaactgttc tgcgcctgaa gtagtcatgg ctcaactata agtgccggag
     4681 ccgaggtcgg taaaatttta ccggcgcagg taatcgagta cgtgggttag gtcgcggtcg
     4741 ttgatggctt tgtcggcggc gagctgggtc tttggcttgg cacagaacgc gagacccaga
     4801 ccggcggcct gaatcatcgg aatgtcgttg gcgccatcgc ccacagccac ggtctggctc
     4861 atcggaatac ccaaacggtc cgcccatgtc tgcaaggcgt gtaacttcac agtcttgctc
     4921 acgatttcgc cgagcacctt gccggtcaag cactcatcgg caacctcaag ccggttggcg
     4981 atccaatagt cgatatggcc ttcggccgcc agcatatcga ccacttcatg gaagccgccg
     5041 gacaccacgc cgatcttcca accgtgttca tgcagcgtgt cgataagctc cagcgcaccc
     5101 ttggtgaaat gcagcttgtc atgcaccgtg tcgaacacgg agatcggcag gccctccaac
     5161 agggccactc gtgcgcgcag cgcatcgcag aactccagtt cgccgcgcat ggcgcgtgcg
     5221 gtcactccgg caatctcctc accgcagcca gccaccacgc ccaactcgtc gataacctct
     5281 tcgtcaatga gcgtggagtc cacatccata accagcaggc cgggctcgct cagcgtcggc
     5341 acagaaaccg cagaaccagc cgggctccca acttcgctct cggcggactt gtcaataaaa
     5401 tgcacatctt cttgcgtcat gcttctgatt cttggaaaac cttgggacaa tgccctctat
     5461 gagtttttcc atggcagccg ccactattct tgcaaccgac cccacaccca tctgcaccac
     5521 caccgaaccc ggcctgatcg gcaccatcac cgactggctg atcatgctga tggaaaccat
     5581 cggcggtatc ggcgtggcct tggccatcgc cctcgaatcc atcttcccgc ccatcccgtc
     5641 cgaagtcatc cttccgctct ccggcttcac cgccgcacgc ggcaccctga gtctgccggg
     5701 cgcaatcatc tgggccacca tcggctccgt actgggtgcc tggaccttgt acggcatctc
     5761 ccgctgggtg ggcctgcatc gcatcaatct cgcggcagac aaaatccccg gcgtcagccg
     5821 caaagacgtg agcaaagcca acgactggtt caccaaatac ggcacatggt cggtgctcat
     5881 cggccgcgtg attcccgtgg tacgctcgct gatttcgatt cccgccggat tcaaccgcat
     5941 gaacttcctg cacttcaccg gctggaccct gctcggctcg gccgtgtgga acaccatcct
     6001 cgtttccgcc ggttacctgc tcggcgacca gtggtgctcg attcaaggcg cgctcggcgt
     6061 gttcgaggac gtggtcatcg cggccgtgat tgtggtcatc gtatggttcg tggtgcgaaa
     6121 agtgcgtact gttatgcgta acaggaaatc cgccgacggc aacgaatagg gtctgccgac
     6181 tgccataagt tgccgaaaat cccggataga agggaagcga agaacccgca tgagcgacac
     6241 cgaagatctc gccgcgctga acgatcgcct gatggccaag aaccatgcgc tcgccgaagc
     6301 gctgagccgc gccggcaagg aactgaccaa ggcgaagtca cagctcgccc agctcgctca
     6361 gccgccgctc accttcgcca ccatggtcaa ggtcgattcc acccgcaccg atgaagacgg
     6421 catccagcac gcgtcggccg aagtcatttc cggcacccgc cgcatggtgg tgcctgtggc
     6481 ctccaacgtc aacgccgccc gcctgaccgc cggggccacc gtgatgctca acgagaagct
     6541 cgtgctggtc gagcagcgcg atgctgacac cgtgggccag attcgcagtg tcaagcaggt
     6601 acttgacgac ggtcgactga tcgttaccga cgcctccggc aatcccgtgc tcatccgccg
     6661 ttccggcgcg ctcgcgtatg cgggcatcaa tcagggcgac cgtattatcg tcgatccgtc
     6721 cgtccgcctc gccatcgaag cgctgcccgc cgagggcgac aaggatctgg tgctggagga
     6781 gaccccggat gtcacgttcg ccgacatcgg cggcctggat agtgagatcg gtcgtatcag
     6841 ggacgccgtc caactgccgt tccagcatcg tgcgctgttt gagcgctacg atttgaagcc
     6901 gccaaagggc gtgctgctgt acggtccgcc cgg
//
LOCUS       DAWBMW010000081         6915 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_2514_length_6915_cov_3.012828, whole genome shotgun sequence.
ACCESSION   DAWBMW010000081 DAWBMW010000000
VERSION     DAWBMW010000081.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 6915)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6915)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6915
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2514_length_6915_cov_3.012828"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3949..3958
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1326 a         2132 c         2072 g         1375 t
ORIGIN      
        1 gtctgagggt ggtccgccat accggcagag accaccctca gtctcacttc gttcgacagc
       61 tcccgccagc gggagcataa ggacttaagt catgcccatt ccactaattc ttgcttccaa
      121 gtccaagcct cgccgtgacg tgctgtattc ggcgggtatc tgccccacaa ttcgcgtctc
      181 gcatgtggat gagcctgctg cgctggaagc ggccgcccgt gaagaaggcg tcaccgtcga
      241 cgatttgagc atcaagcagc gcgtgatgat tctggccgcg gccaaggccg aggccgttca
      301 tcgtgcgtat cgtgatgtgg ccgatactgc cgccgctgcg accggtgacc gcgtgatcgc
      361 ctacccgctc aaggccaagg aaatcaagga ttcggaacgc gaagctgccg tcgaagactt
      421 gcgtgaagcc gccgctggca agcccattga ctattccaag gcggaaatcg ccaccacgcg
      481 cgacttctcc ggcatcgata tgccgaccgt caccgaaccc atcgccaccg ctattgccgg
      541 acagccggga ctgaccgagg cgacagtcgg cccgctgatt ctcggttgtg attcgatgtt
      601 cctcttgggc ggcgaatgct acggcaagcc gcacagcgaa gccgtggccc gcgaacgact
      661 gaagcgcatg agcggcgcga ccggtgaact ctggaccggc cactgcctga tcgatttcgc
      721 gaccggacgt actgtgcgcg gcgcaagcca tgccaaagtc catttcggcg aattcaccga
      781 tgacgacatc gaacggtaca tcgtcaccgg cgagccgctg gaagtcgccg gatcgttcac
      841 gctggaaggc ttcggcggcg cgttcatcga cagtattgag ggcgatccgc acggtatcat
      901 cggcctcagc ctgccgctgg cccggcatct ggccggcgaa ctgggcatca cgtggaccga
      961 cctgtggaat gtcggccgtg gcgaactgga gccggaaagc aaggcctcgg gcaaccagca
     1021 cgctggcatt ctgccgccgg tcgaaaacgt gcatcagccc ggcgatggct gggtggactg
     1081 cgcctgcggc cgcaagcatt ggggcaccaa cggtgcgtcc ggcattctgc tggctcgccg
     1141 cgacccggcg agcggcaagg tgacccacgt ggtcatgcaa catcgcgccg cgtggagcgc
     1201 cgaaggcggc acgtggggta ttcccggcgg tgccaccgcg gacggcgaat cgcccatcga
     1261 gggcgcgctg cgcgaaagct atgaggaagc caacatcact cccgaagaca tcgaggtagt
     1321 cggctcctac cgcgaggacc atggcccgtg ggcctacacc accgtgtttg cctttgaaaa
     1381 gcccggccac accgtcgagc ccaaggcaaa cgacgacgaa agcatggaaa tctgctgggt
     1441 cccgattgac gacgtgccca accgcaagct cctcaccgct atgaagaccg actggccccg
     1501 cttcgccgcc cgcctggacg agctggcctg tgctggacat cggtagtggt gttggctggg
     1561 ccaatgtttt gccgatgagt ttacttgggt aatgccgtgt gaaccgcgtg tggaccggga
     1621 cggattctgc tgtcccggtt cgtcatttcg tctcttttgg gacaaccggg acagatttcg
     1681 ctgtcccggt tcgtcatttt gcctcttttt gggcaaccgg gacagaatct cattcacctg
     1741 acgccctatc gtgcgatggt cgccgtcggg gaatggcgga attccggggt tttggatttg
     1801 catatatcgg gtgttcatgg aagggcctgt cccggttcgc caaaagaggc cgaaatcccg
     1861 aaccgggaca gccgaatctg tcccggttcg tatgtcgatc gattgacccc gccaccggga
     1921 catcaaccgc gcatagacag gacgcttcaa tgcctggcgg gcatgaccat atcggatgac
     1981 acacgtttcc tcgtgcgttt cgccacgacc tccgccccaa gtcagtgcga aaatggcatt
     2041 tttagcggtg tggtcagctg tcgcatgcgg aaaatcctaa atcaaccgcc gaagagacac
     2101 atcgccgact cggcggaact cgctggtctg cgcgcaatga ggagtgttgt gtctgaatgc
     2161 gattcagaca atggtcggcg tcaaatagcg gtctccagcc attaatcatt ggtgattgtg
     2221 gacattgagc ggagtaaaca ttctagatga ttcagggata tgccgaaaat catatcgttc
     2281 agagaatgaa gtaccgcatc tcggagtaac tgactgtcga cgtgcatggt ttgaaatctg
     2341 atatccacgc ccatcatgcc tagcaaaatt acgggaatgg ggttcggact atttcgcctc
     2401 agccaaccgg gagcttccga atgctcaatg ctgtatgcac ttagtgtgtg gaaaccagcc
     2461 aatcattgac caatggcagg ccgcagtcga aaccagaaat atcctcgctc gcagtcaggt
     2521 gccgaggctc agtgaagttg gtcacgtcca tatgggatca cgctccaaca attcattcag
     2581 acgtaggccc ataggttcgt ttaatgctgc ggtgaaatca ttccacgctt tactgttgag
     2641 ccgaatgata tgagcttcat ggatgtcccg atctgcggcg gcgagcagat tcgataatgc
     2701 ccattgcaac gaagtcatgc ctttggactc ggccgcttgg tcgattctgg aacgttgctc
     2761 ttaggtgagt ctcatttcaa aacgactgtt cttgttattc tctgtagtca tactcctgtt
     2821 gtacgacttt tgtacggaca cgattggtgt gtgcgggatg tcgattgcga tggccatgtg
     2881 ggctcctttg cttggtctgt tcgatgtaca ggcgcgcagc gcgctggttc cctgaacata
     2941 ccgtgcgtgc gattcgcata tccagccgaa ccggcctatg tggttccagc tcccgaaccg
     3001 gtcttccact gttcctcgtc ttctcgagca atgaacatct gatatatgga ttgtcgcgtc
     3061 gactgcgtat ctctaatcct tgcggatgag gagcgggccg gtaatccaga aaggttcata
     3121 ccgcagcccg atggtcggct gggcgttcgc tccgtacgct cgggagtatg aatacagcag
     3181 catcgcaatt gcagcagcct cagccggcgc cgggaccggc ggctcagcct cagcagtctc
     3241 agcaacccca acagttccag cagccggcat cgcacaccca gcagtgggcc ccggtcatcg
     3301 aggcccatga cctggtcatg gactacaccg ccagcatggc ccgcgcacag gccgggcacg
     3361 gcgtcaccgg cgtgatacca gccggcacgg gagccggtta tgcgtcggcg aatcccgctg
     3421 cggccggacc gggtgccatt caggccggcc agccatccca gccagcccag cccggcttcg
     3481 ccatgccgac catgcacacg ctcgccctga accacgtgaa cttcacgctc cgggagggcg
     3541 agacggtagc cgtgatgggc ccgtccggct ccggcaaatc cactttgctg cacgcgctcg
     3601 ccggcatcat taagccgacc gccggcaccg tcatcttccg tggtgccgac ctgagccgca
     3661 tgagcgacgc cgagcgcacc aaactgcgcc gcaacgactt cggcttcgtc ttccagtccg
     3721 gccagctgct gcccgaactg ccggcggtcg aaaacatcgc cctgccgatg atgcttgacg
     3781 gcatgcccta ccgcaccgcc acggacacgg cgatcctgtg gctggaacgc atgggattgc
     3841 gcgcgctcac cacgcaccgg ccgggggaga tgagcggcgg acagatgcag cgcatcgcca
     3901 tcgcccgtgc actcgccgtc aaacctgccg tggtcttcgc cgacgagcnn nnnnnnnnac
     3961 tccgcccccg ctggcggggg ctgtcagccg caggctgact gggggtggcc cgagggcgct
     4021 gaaccaccct cagcctcgct acgctcggca gctccagccg gcgggagcga aaccaccgca
     4081 ggaggcgcgc gatgagcacg ttcgccctct ggcggctgtt tcaccgcccc ggcttgcgcg
     4141 gcagcgccgg ccacacctcg atgctggcaa tcatcgcctt cgccgcagcc accaccatct
     4201 tcctcaccgt gctcggcggc gtacacggtt tcatctggcg ggcttcggcc gatcataccg
     4261 tcgcctgcgc gttcgacttc ggtgcctgcc ggcccgatac ggtggccgcg tggaaacggc
     4321 agatcagcga cccgacgagc ctggcccaat tctccaccgc ctatgtggcg ttggcggtat
     4381 tcgcctgcat gctgctcgtc gtgccgttcg tggcgctcgc cggttccgcc gcacgactgg
     4441 ccgcatcccg ccgcgacgcc cgtctcgccg ccctgcgtct ggccggcgcg accaccggtc
     4501 aggtggtgcg tctcaccgcg cttgacgccg ccggacaggc gctgatcggc gcgcttatcg
     4561 gcattgccgg ctattgcgcc ctgatgccgg cagtcatgct gattcccttc caggagcagc
     4621 ggttcacctt cgaacagcta tgggtcggcc cgctggcgct ggtaatcacc ttggcgggcg
     4681 tggccatctt ggcgctgata tcgtcgctgg tcaccttgcg ccgcgtggca atcaccccat
     4741 tgggtgtcac ctcgcgcacc gccaccgcat tgccctcagg cacccgtatg gtgctgttcg
     4801 tggtagtcgt ggtggtggcc atgatcgtct tcaagaaccc ggccatgctg gctcaggccg
     4861 gccaggcggt gatgtacggc gtgatcatcg gtttcatgct gctgtgcttc gcgctggtga
     4921 atcttatcgg cacgtgggtg atcaccgctc gcgccaaggc caaggccaag cgccccaagg
     4981 atgcggccac gatgattgcc atgcgccgta ttctcgacaa ccctaagcgc gcctggcgca
     5041 atgtgtccgg cattgcgttg gccgtgttta tcgccggcat cacgtcgatc tgcggattct
     5101 tcggatcggc cgccgtggtc gatgacgcca acgacccgtt caccgtgttc attcgcgaca
     5161 tcggcaccgg cggcattctg acgctcgtgt tcgccgctgt gcttgccgcg gtcagctccg
     5221 gtgtgatgca ggccggcagc gtgtacgatc aggccggcga atatcgcatg ctggtgttgg
     5281 agggcactga tgtcaagacc ttgaaccgtg cgcgattcat cgaggtactc accccgctca
     5341 acatcgtggt gatcgtggcc ggcggctgtt ccatgctgct tatggcccct ctgttcgccg
     5401 cttcgatgac cgaaccggcc acgctggcca gcttcttcgg cgggcttgtg ttgtgctatg
     5461 cgctggtttc catcggcgcg ttcgcgtcca atcatgtggc ggccagcttg aatctgaccg
     5521 attatcgggc tgatgactga gccgagctgc gcagcgaatc gttctcaata acaactgtgc
     5581 gtactttcgt atacgcaaat cgaacgtaga aacgctgaaa tcaccgcaaa tatgcggatg
     5641 agcaaatgtt cgtttgtgcg atatctgtgc atttgcgcgc acggatacta ttgagaacgc
     5701 ttctctgcgc acccatcacg acagcagcgc cgcgatgatc gcgataaaca ccgggctggt
     5761 catggtgctc atcagaatgc cgtcgcgcgc gaaggtgaga cccacgttgt agcgggccgc
     5821 gtagttgtat acgttctggc cggtgggcag agcggcgagt accacgcagg cgtacagctc
     5881 ggagccgcgg aagcccatca cgaagtaggc gagcaggaac gcgataattg gcatgatgat
     5941 gttcttgagg accgcgaccg tgaagatggc cgggcgatcg cccttctgct gtagcggctt
     6001 ggtgccgtgc agggacatgc cgaacgccat caggatcatt ggcacagccg aatcgccgat
     6061 catgtcaatc gggtcgaaaa tgaatttcgg cactgggaac cagccgcttt tagcggaaat
     6121 cgcagagacc acaatgccca gcagcgagcc gataagcagc ggctgatgca gtggctgctt
     6181 ggcgatttcc ttgatggaga ccttgccctt ggtggtcacg tccagcactg tcaggcccac
     6241 cggtgtgaag atggcctgct gcatgaccag aatcggtgcc acaagagccg gattgcccag
     6301 aatgtaggtg gcgattggca ggccgatgtt attggaattg aggtacagcg aattcagggc
     6361 accgatggtg gcgtccgggg cattcaggtg gaagaacata cggttgagca ccaaaaacac
     6421 cacgcctacc agcacggcgg agaagaacgc cacgataatc gaggggtgga agatgtcgaa
     6481 aatcggctcc ttggagagaa tcgcgaacat caggcacggg ctcgatacga agaaggagaa
     6541 ccggttgagc accatctgag cggacggccc gccgattcgc attcgtgcgg caacatatcc
     6601 caccgcgatt acgatgccga taacgcagaa gccttgcatg gcgctgatta gacctggcat
     6661 aatcctccct ttcgccaccc cagtgtggca cataaaaatg tattcggccg caaaatctca
     6721 tgtagtgagt gtgctgggcc cgccgcccac gcggtacgct gttcacatga cgcttgaatt
     6781 gaaccgcacc gcatccactg ccgagcagct gaacagcctg ctcacccaga tcatggagaa
     6841 cttctccgtg tccgaccacg agggccccct caccgacgag gtcgaggcct tccttaacga
     6901 gcaagagcac ctgac
//
LOCUS       DAWBMW010000082         6901 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_2520_length_6901_cov_3.388110, whole genome shotgun sequence.
ACCESSION   DAWBMW010000082 DAWBMW010000000
VERSION     DAWBMW010000082.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 6901)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6901)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6901
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2520_length_6901_cov_3.388110"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2168..2177
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1334 a         1963 c         2135 g         1459 t
ORIGIN      
        1 aggtgctgtt cgacctcgag ggtgatctcg gtctccttcg cctcgccttc ttccttggcg
       61 ctggtgttga cgatcgttac tttcagggcg ttgtagatgt cgggcagatg acccaccggg
      121 aattcaacgt cgataaccga gccctggacg cgggtgacgc gaccgttggt aggctcggcc
      181 gcagtggtct ggttgtcagc catatgcgtt cctactcttc cttcttggtc agcgcgtcag
      241 cgctgccgat gatttcggta agttcctggg tgatcgaggc ctggcgggaa gcgttgagct
      301 tgcgcgtaag gtcgtcgatc aggtttcgtg cgttgtcggt tgccgtgtgc atggcgttct
      361 ggcggcttgc ggtttccgac gcggccgcag tcagcaggca ctcgtggata cgggactgaa
      421 tgtacttcgg cagaatcgca tccagtacct tttcgaggct gggctcgaaa gtgtacagcg
      481 gagcgacatc cgcagtagcc ggtgcctctt catcaggatc gggcaccttg gtctcgtttt
      541 tcacgatttc gaccggcagc atgcgcagca cgcgcacctt ctgcaccacc atgttgatga
      601 actcggtgta gacgatgtag agttcggaaa ctccaccctt ctcagccggc ttcatgtagg
      661 cgtccatcaa ggtgttggag atcgtttcag caatttccac gccgggctgg tcggtgtcgc
      721 cttcccacgt cgccgcgatg tcacgattac ggtacttgta gtacgtcgaa ccgcgacgcc
      781 cgtacacgaa cagctccggc tgcttgcctg ccgcatcgag gcgggacagc agcgactccg
      841 tttcacggat aatcgaagag gtgtacgggc cggccatacc acgatccgag gtgagggcga
      901 gaacagccac gcgcgggttc ttctcgtcct tcaccgcgat cggatgcgtg atatgggtgt
      961 gggccaccaa cgcttgcacg gcatcgaaaa tcgcatcggt gtacggcttc gcgttcaggg
     1021 ccacatcacg ggccttggcg atatgcgaag acgcgatcat ctcctgagcg ttgaagatct
     1081 tcgccaacga ctcggtggag cggatgcgtg atttcaatgc gagttgcgag cccatggctt
     1141 acttttctcc cgccacgatc ttttcctgat cgaccggcgc ggccttgtca acgtccggct
     1201 tcttctcgac cagcggcttg ccggcggaag agacgaacgt cgaacggaac tcgtccacgg
     1261 ccttatcgag ggcagcctcg gtgtcggcgg tgaattcctc ggtctcgcga atggtcttga
     1321 ggatatcggt gttgtggtcg atgtagtcca gcagtccctg ctcgaacgga aggacgtcct
     1381 tcaggtcgag gtcgtcgagc ttgccgtggg tgccggtcca tacggacacg acttcctgtt
     1441 ccggcgagta cgggtggaac tgcggctgct tcaacagctc ggtcaggtgg gcaccacggg
     1501 tcaactgggc cttggaagcc gcatccagat cggaggcgaa catggcgaag gattccagcg
     1561 aacggtactg tgccagggag atcttcagcg taccggagac cttcttcaga gccttggtct
     1621 gtgcggcgcc accgactcga gagacggaga taccgacgtc cacggcgggg cgctggttgg
     1681 cgttgaacag atcggactgc aggaagatct ggccgtcagt gatggagatc acgttggtcg
     1741 ggatgtaggc ggacacatcg ttcgccttgg tctcgacgat cggcagaccc gtcatcgaac
     1801 cgccgcccag gtcatcggag accttggcgc aacgttcgag caggcgggag tgcaggtaga
     1861 acacatcacc cgggtaggct tcacgccccg gcgggcggcg cagcagcagg gaaatcgaac
     1921 gataggcctc tgcctgcttg gacaagtcat cgaagacgat gagcacatgc ttgccgttgt
     1981 acatccagtg ctggccgatg gccgagccgg tgtacggggc gatgtacttg aagccagcgg
     2041 aatcggctgc cggggaggcc acgatggtgg tgtactccat ggcgccggcg tcttccaggc
     2101 tctgcttcac cgaggcgatg gtggagccct tctggccgat ggccacgtag atgcagcgga
     2161 cctgcttnnn nnnnnnngca cggaaagcac ttcgccggtg cgtcgcactt cctggccttc
     2221 ctcaattcca gcgaaatcgc cgaggatcac cacgccgatc tcacgggcgt caaggttgaa
     2281 cgccaggccg agcgtgccgt cttcgaacgt cagcagctca ttggccatgc aaccaggcaa
     2341 tcccgtcacg tgtgcaatgc cgtcaccggc cgtggcgaca tagcccactt cctgggtggg
     2401 ggtgtcgctc ggcttgtacg acgacacgaa gtcgtcgagg gccttgcgaa tcgaggcggg
     2461 atcgatggtc agttctgcca tgatcactcc ttctattggt tattgttcag tcaagtcttt
     2521 gcttgtgtgt ggtgttggac ggattggctc ggccggtcag gccggagtcg cctccgtctg
     2581 caccttgcga tgcaagttct gcagctgtgc gaccacggtg ttgtccgtga cttcgtcgcc
     2641 cacctggatg cgcatgccac ccagcacggt cgggtcgacc accgagttga tgtgcacctg
     2701 acggcccacc ttggcggagt agacctcgac cagtcgcttg atctgctcct tcttcagcgg
     2761 ggtggcggtg gtcacggtga ccatcgactc gcccatgtgg cgggagaact tgttgatcag
     2821 ccactggatg gtctccaagt agcggcggcg gcgcaggttg caagtggcgt gttcggcgag
     2881 cctcatggtg accttgttca gggtcttgcc agagaacacc tcgcggaaca gcttcacacg
     2941 ggcctcgctg gtggcctggt agtcgtacag cttggcgcgt acgaccggca ggttcagcag
     3001 tgcggaatgc agctcggaca gctcgatgga tacctgcagc gtggcgtcag tggcgtccgc
     3061 gtagtacatc atggcgtcca cgccgaagtc ttccactgcg ttggcgatgt cgcgcgcacg
     3121 gctccaacgg cgggagacca agtcagtcat gatctccatg gtcatcggat gcgcgttgtc
     3181 accgagcagt tccttcagga cggccacctt gtcggcaacc gggcgggacg ggtcggtcag
     3241 tgcgcgctcc agctgaatgc tgtcatcgag caccttggtg atcgtgaaca gttcgttgcc
     3301 gatccgccac gcgtcctcac gggtgtcgcg cagtttcggg gccagcgaat cacgcgactc
     3361 gcggtctgca atacgtgatg cctctcctcg catggtcacc tccctttctg gttacggatg
     3421 gtcacggtca cttcttggcg cccaggtcgt cgatcatcga atcgatcatc gaggactgca
     3481 cgtcgttgtc ttccagcttg gcaccgagga tcttgccggc cagggcggtg gcaagtgcgc
     3541 cgacctcgcc cttgagcgag acgatggcct gctggtgctg ggattcgatc gagcgctgtg
     3601 cggatgcggt gatctgggcc gcatcggact cggcgcggga acgagcgtcg gcgatgatgt
     3661 gggacgcttc cgcacgggcg tcgtcgcgaa tcttggcggc gtccacacgg gcggtgctca
     3721 gctgggcctc atacttggcc ttggcctcgt cggcgtcctt cctagcctgc tccgccttgg
     3781 cgatgttgcc ctgaatcttg gcggcgcgct cgtcgaagat cgcattgaac ttcggcagga
     3841 agaacttgta gaagaacacc gcaacgatga caagaatgat cagcgaccat acgatgtcat
     3901 aaacctcagg aatgaacagg tcaatcccgc ttgctgcttg tgtcatcaag ccctcctttc
     3961 agtgatggac ataatcggtt ttcgtctgtt gatctgcgca gatcagatga tcagggcggc
     4021 gacgaagccg atcagtgcca gcacctcgac cagagccagg ccgatgaaca tgatggtctg
     4081 gatcttgccg gacatctcag gctgacgagc ggtggattcc atggccttgc cgaagaggat
     4141 gcccagacca atgccagggc caagagtacc gataccgtaa ccgatgacgg acaggttgcc
     4201 ggcgacctct gcgagggtga tgatatccat tgtgtttcct ttctaacgta cttggcgacg
     4261 ttataaatct tggggtttgg tcggtctgcg attttcccag accggggacc ggaagcggcg
     4321 ggttagtcgc ccgtcactct tccgggaagc tcaggttgat gtacacggtg gaaagaatcg
     4381 cgaagacgta ggcttgcagg aaggcgacga acgcctcgaa gcaggtcatg gcgaaaccac
     4441 cggcgaacca cagcgcaccc accgggatgc cctgcagctt gttgaccgcg tcaaccatcc
     4501 agaactgtgc gaacgccagg caggtggcga ccataaggtg gccggaaacc atgttggcga
     4561 acagacggat ggtcagggag gccgggcgga tgatcaacag ctcaagcagg ttgatcggag
     4621 ccagcaggaa atagaccggc cacggcacac cgggggtgaa cagctcgtgg cgcagatagt
     4681 ggccgaggcc ctgcgagcgg atggcggcga tccagtactg gatcagtgtc cagacagcga
     4741 acacgagcgg catgaccacc gtggcgttgg ccgccatgtt catacctgga atgatgccgc
     4801 acaggttgaa aatgaagatc gtgaagaaca gggtggtgat catcggcacg tagcgcttgc
     4861 cacgggcctc acccatcacg tcatacacga ccttgtcgcg cacgaagtcg agaccgtact
     4921 cgactacgcc ctgccaacgg ccggggatga gcttggcgcg ctttgcggtc acgccgagca
     4981 ccaccaacag cacgatggtt gccacgatac gaatcaaaat aatgcggttg atggcaaacg
     5041 gagttccctg gaacagaatc tccgggggaa ggaagtcatc aaccgagggc agatgagcgc
     5101 ctgcctcgtc agccaacagc aatcctgcgc tgagcgaacc gaccatatca cgcctcctgc
     5161 ttcttcaatg tcaggtgtca gtttaaaccc accgggcagg caaatgtgac gtgctcgtta
     5221 ctttcgtctt tttttgtctc ctcggtgtgg agtctgccgg gcatggacac ggagtctgcg
     5281 ctgggcattc ggctccgaat gactgaagag tgattatttg cctgcgtcta gacaatgtca
     5341 atattatacc ggcgagtctc ctgaaatccc tactgctgca aggttttccc gggcgtttcg
     5401 cgaacgttgt gcactattgg tcgcattacg cggatgtgtg gcattacgtt catcaagccg
     5461 tccacatcgt ggacgaacaa ccgtccacgc tacgaacgca acctccaaca gtagctccct
     5521 ctgatgggcc gagccgtgaa ggagaagcaa aggagctccc cgtcgagggg agctgtcgtc
     5581 gtaggcgact gaggggagat cgtaggctca gccctgaatc accccatacc cgtcatgcac
     5641 aaacggcttg aaatcatcct ggcgcggccc gcccggctca tgccgaatcg aacgatcagt
     5701 accgagcttc ttctcagccc tgagctgcgg cacctcggtc aggtcatacg gcgtggtctg
     5761 gtacacccag ttgagccagt tgcgccacag cagattggcg tgggcgcgcc aggcgaacaa
     5821 cggttccagc ttcggatcgt catgcgggaa gtagttcttc gggaagggca cgttggtcat
     5881 gcccttggcc atatcgcgct cgtattcttc ggcgagcgtg tacttgccgt actcccaatg
     5941 gccgagcgcg aacacttcgg agaagtcacg cgtggcgatc aggcccgggc cggactgcgg
     6001 cccccaagtc aagatctgaa ggtcatggtt ggcacgtacc tcgttttcgt tcacgccggc
     6061 gaggcgggag tgcggctgca gatcaatctc gtcgaagcca ttggtcagga agcagtattc
     6121 atcctgcagg tactgcggga atacgccgaa aatcttctcg gggtaatcca ccttgtggat
     6181 gccgtagcgg tagtacagtg cgcccatcgc accccagcac aggtatatgg tggagaacac
     6241 atgggtggag gcccagtcga gaatcgtctt gaactcgtcc cagtagtcca catcttcgaa
     6301 cggcatatgc tctacaggcg cgccggtgac cacaaaaccg tcgtaatagt tgtctttgaa
     6361 cgcatcgagg ttttcgtaga acttgacgag atgatcggcg gaaacgtgcg tggcctcatg
     6421 cgtggaggtc ttcatgaagt cgatttcgac ctgcagcggc gacttggaaa tcagacgcag
     6481 cagctgtgtt tcagtctcga ttttcttagg catcaagttc aggatcacca gtttgagcgg
     6541 gcggacgcgc tgacgctccg cctcgggctt ctccagagcg aagatgcgct ccgaatcgag
     6601 gatatctctg gccggcaggc cactggggat cttgataggc atgttcctat tatgtcaata
     6661 ttcgggataa cggttcaggg cattcctata acggccattt atggcgcgtt attaccccga
     6721 ctccgactcc gccccaaacc cctcgctgcg tgagccaatt cacgttttaa acccgcggga
     6781 agatgacgcg acacgccgat gttgactttc tggaatctgc acctattgta aatagagctg
     6841 tctgagagac aagccgacgc ggggtggagc agctcggtag ctcgctgggc tcataaccca
     6901 g
//
LOCUS       DAWBMW010000083         6871 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_2533_length_6871_cov_2.707160, whole genome shotgun sequence.
ACCESSION   DAWBMW010000083 DAWBMW010000000
VERSION     DAWBMW010000083.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 6871)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6871)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6871
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2533_length_6871_cov_2.707160"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    356..365
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2265..2274
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5087..5096
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1333 a         2121 c         2075 g         1312 t
ORIGIN      
        1 acgaaggtct tgggcctcgg gtcgaggtcg cgcagcagca gcgggccttc gggaaaacca
       61 tggtcagtga tgaagttgcg aatcgaactc tccacattcc agggcgatgt cgaaagatag
      121 aagaacggtg cgccggggaa caagtcggcg atgcgggtga acagcacgct catgcccggc
      181 acggaggctt tcttcttggg gtcgaggaac agcaggttgt acgcggcctt ccatggcacc
      241 ggggcctgcg tgatcatgat ggtgtcgtcc acatcggaga tgatgccgac tttggcactg
      301 gcggggatgg tgtacaggtt ggcgctcacg ggtttgcggt tggccacgct gtaggnnnnn
      361 nnnnngtagg agacgcgatg gatgccgggc accagacgat gctcggccac gaggtcgaga
      421 tatccggcgg aatcggagat ggcgtattcg ctgtcgagat cgcgcgtgcg gtccaccttg
      481 tcatagacct cggagacgcc gatctgcacg gtgttgagcg gcatgtcatc aatactggcc
      541 tgcacgtggg tgcgcggcgc gggtatggca agcatgccgc ggatgccgcg gatcagttcg
      601 ccggattgcg cgtgcctggg ggcgtatacc gtgcggcaga tcagacgcga gtaatcgccg
      661 gtgccataac ccacataggg ttccactcgc ggataccagt cccattggcg caccacctga
      721 gtggagatgc gtgaccatac gccgaacacg cgggtgacgg cacgacgtat gaatcgggtg
      781 atcggcggct tgacttcgat gcgctcacgc ttggaggggg tgtcgaacgt cgtggcagcc
      841 ttgcgtgcgg gaatcggcac ggtgggcatg tcatcggaat gttcagcgtt gctggcattg
      901 tcggcaccat tggcggatgc cttgacgtca gagactggcg cggtggcgga cagcgctgca
      961 ctgcgagcag cagcatccca aggcagtcgc ttgcctgccg cctcaaaatc ctcgcgtggg
     1021 cttctcatag acaccaatat aggccatacg cctcattttc ttggctcccc tcagagaggg
     1081 gagctcaaaa ggaactaccg cacgaccttc gcgtattcgt cggcgctcag cagctccggc
     1141 tcatcgtcgt caagctcaac cttcagcagc cagccttcgc cgtacggatc gccgttgacc
     1201 acggacgggt cgtccgccac gtcacggttc acatacttga ccgtacccgc cacggggctg
     1261 atcagcggct ggacggcctt ggaggattca agctccacga tctcgtcgcc ggcctccaca
     1321 cgggcgccag gctcaggcag atccacaaac acaagttcgc caagctggtc ggcggcgtat
     1381 tcggtaatgc cgatgattgc ggggtcgacc gaacggtcca cccatgcatg ttcctcggag
     1441 tattcgaggt ggtcgggaat gtcgagattc agcggctgtt cgttttcgtt gtcatcactc
     1501 atagtcataa gcctaaccta tcttcgcggt ccgtcacagc tcgcgaccga gccattcctc
     1561 aatcatgtag cgggcgatgg tggcgcggcc cggcgccgcc atacggccgg cgatgagctc
     1621 ggccgtgtat tcgtcgcgtg tgacccatcg cgcggtcatg gtctcctcgc cgtccacacg
     1681 cacgtcggtg gtgatcgcat gtgccttgaa cgccatcatc agcgaagccg ggaacggcca
     1741 cggctgcgaa cccaggtacc gcacctcgcc aagcttgatg ccggtctctt ccatggcctc
     1801 gcggcggcag gcgtgttcaa ggttctcccc cgcctccaca aatccggcgg acaccgaata
     1861 cagcctcgaa tccttccatg ccgcgttgtg ttgcagcagc agacggtcgt gcccgtccac
     1921 gatggcggta atgaccgccg gctccacgcg tgggaacagc acacggttgc cgtccgcctc
     1981 gtttgtgcat cgctgcgccc atccggcgag cgccggccgg accgccgcgc cacaggtggg
     2041 acagaaccgc tggcggctgt gccagatgct taaggtgatg gcactggtgg cttgcccggc
     2101 ctcgcgcgcg ttggagtgag gggcaaatcc gcgcaggtcc acccagtcga accgggtcac
     2161 ggcctgttgc agcagcgtgg gcttcggggc caagggctgt acgggcgcgc cgaccgaatc
     2221 atcgaaggca tcgtccgcac cttgcccggc cgaatccggt acacnnnnnn nnnngatttt
     2281 ggctgcggct acgccgccat tacccttggc gtttcccggc tgccgtattc cggcggcagc
     2341 acctccgctg atgccgttct tgcccagcgc gccgtccaca gtgccggaat cctgggtgcc
     2401 gagcacgccg ccgacggcac cgccaacacc ggcaatcccg ccaacaccgg gaacgccggc
     2461 ttccggcacg cgcgtgatat ccacggcgac cacggattcg ccgcgcgccc cgacatagga
     2521 gccaaggaac atggcgaccg cttcggggta gcgtgccagt tcggcggaca cataggcgcc
     2581 cggcaacgtg gcgagccgca ttttcacgtt ctcatagtcc acaagctcgc cttggccgcg
     2641 cggtacggcg acaagtccgc cgcgcgtaag cacgactttg gtggtcggct cggccagcag
     2701 ctcgtcgatc aggccgggtt cgcttcgccg gtccacctga tagtcgatgt cgccttgtgc
     2761 gagcggcagg aacggcagcg catgggtcag tgccagaggc gagaaatgaa tgtcagcggt
     2821 catcgaggtc tcctatgcgt tcttctgcgc gctctgcacg cgcttgacga gcaagagcag
     2881 tgctcgcgcc acggtatccg gatgttcgac ggcactgaaa tggccgcagt ccggaatatc
     2941 gatgaattca gcgccggtga ttcgttcggc gagcggccgc atcacactcg gattgctgga
     3001 ggggtccagt tcgccggata ccaccaggct cggtgcgctg atcgtttccg gcaccgcccc
     3061 cagttcaggc cgtccgtagg tcatgcgctg acgccaagcc agaccagccg ggttctgttc
     3121 gttgatccag ccggtcatgg tcgcgatgaa ttcaggagtc tttttgacgg tggaatcgcc
     3181 atcggccggt tgcgcgaagt gcatcaccgg ttcgaccgag ttcgtctgtt cggcggcgtc
     3241 cgccatcttc agtcggttct ctccgcccac gccgtcggac gcggccatcg tgtcgcacaa
     3301 ggccagtccg gccaccgttt cggggtacag gcgctggatg tccatcgcca cgtagccgcc
     3361 catcgacagc ccgacccaaa tcgcctgctc atagccggcg gccttgatca ggtccacgta
     3421 ggctccggcc aggcgatcca acgcctcgct gtacgcgccg ttcgccatgc gggggcccga
     3481 atcggcgtcg ctcggcaccg gggatgcgcc ggagccggcc atgtccggag cccagatcgg
     3541 gaagtccggc acgtcgttca ggtcggcgaa gtcggcgagc gccttcgcgc acacgtccca
     3601 catgcggtgg tccaccgggt aggcgttcat cagcaccacc ggcacgcctt cgccgtcgcg
     3661 gtagatatga ttggccaatt cgattgccat gaattactcc ttcacattcg cgccccactg
     3721 gcggagctgt cggcacagtc ggctgggagc ggtctcaacg cggaccgccc tccgtcgcta
     3781 cgcgccatcc cctccaacgg aaggcaaaac aactgtcaga ttccggccca ggccaacgcc
     3841 tgtcgcacga tgcgcccatg gccgttgacc atctgcgcca cgaacgtgtc cattacgtcg
     3901 ctcttgggat ccacccattc cacatccagc gtctcatcct gcggcttgca gtcgccggcg
     3961 ataggcacca cgtagcacag tgcgatggcg tgctggcgcg gatcgtagca ttcgcttagt
     4021 cccggcgtgg ggaagaattc ggccacggtg aacggctgga gcccgatcgg cagcagcggc
     4081 aaggcgatat cgcccaaatc cttggccaca ttgcgggcga tggcctcgcg caacgtctcg
     4141 tggtacagca cacggccggt gatcagcgta cgctcgatcg acccgtcctc ggatacgcgc
     4201 agcagcgagc ccacctggga gatgcggccc atctcatcgg tgcgtacggg cacgatctcc
     4261 acatacgcga tgggcatctg atggcgcgcc cggttgatgt cggacggccc cagccagccc
     4321 ggcgggttgc catggccaga accgcctccg tggatgaagt cctcgggcgt gatgttgtcg
     4381 aattcgccgc ggccacggcc ggcatcataa tcgccctcgt cgggcacttc atcattcatg
     4441 actggcatgc atcccattat tgcgcgtcac agcgcgccgc gcgtcacctt ccagcgaaat
     4501 cttgggaaac gcttggccgg cggcggaaaa tacgacacaa atgttgggtg gtgctgggaa
     4561 gctggtagcc agcagggagc cgacggccac gaaccgcacg gctccaagcg ccaagacgta
     4621 ttgcatgaat aaggcaatcg cccggcggcc acgcacgagg gccgtacggc gacgccaagg
     4681 aaaacacaaa agaagggaac atcatggcaa ccaccgccat cacctccgcc gatttcgaga
     4741 agaccatcac cgacaacgaa atcgtgttcg ttgacttctg ggccacctgg tgcggccgcc
     4801 gatctggacg atctgatcgc ccaggccaag gcactcgatc tggccgccgc caaggccgcc
     4861 cagtccggtg ccgaccagca ggcctgagcc tgattttcct tcatccactg cgccgcctat
     4921 catggtgggc agtgtgaaat ccgatcaagg agcgcaatga cgagacatgc caagccaacc
     4981 cttatgctca ggccgttgaa caagcgccag tggatgaaga tcgcggctgt ggtggccgcg
     5041 gcaggtctgc tcggcacggc gggattggtg tcgcgcagtt tctaccnnnn nnnnnncaag
     5101 aacaccacgt tcgttacggt gaagattaat ggcgattcgc gcgtagtact tggcgaaaaa
     5161 gccaacatga ccaccgtcaa ggacgtgctg gagacgggtg atatcacgct tgacccggat
     5221 gatgccatat ctccctcgct gaaatccaag gtcaccgagg ccaccgtggt caccattaat
     5281 cgcgcggatg ccgatgtgga gaccaacgat accgagatcg ccttcaatga agtgcgcaag
     5341 gagaccagca gcctgcccaa gggtcaggaa aaggtcgaga ccgagggcca gaagggcatt
     5401 atggagacca cctcgctggt caagcgagcc ggcgacaagg tcgtctcttc caacgtattc
     5461 gcctcatggg tcaagaaggc cccggtggat aaggtgatcc tagtgggcac tggttccacc
     5521 aagtcgccgt cgtcggccga tctcggcacc actgttccgg ctggagaagt gcagtcctgg
     5581 gcgcatcagt atctgttgga caacgggtat agcgaagcgg acttcaccgc tgcgaactac
     5641 atcatcaacc acgagtccgg ttggagcccc accgccacca accccacgtc tggtgcttat
     5701 ggcttgggtc aggcctaccc cggtagcaag atggcttccg ccggtgcgga ctggcagacc
     5761 aactacaaga cccagttcaa gtggttcatc agctactgcg agcagcgcta cggcggcatc
     5821 gtcgcagcgt acaactactg gatcgcccat aacaattact gattgcgatt attctcggtc
     5881 tacgcaaaga gcctccgccg gttggcctaa ccaccacaca aggggttacc cccctaactt
     5941 tataattgcg tatgcaattc aatatcgctc gcataattca cggagacggc catgaccttt
     6001 aatcggtata tttgctttcg tgccatcctt gaatccatag gtgccattgc ctgcatgctg
     6061 atggctctta ttggcaataa cctgctcgga gtaatcgtgg cagcgatcgc ggcgctgttg
     6121 tggatcggcg gcgaagtatg gggcatgact gtattccgac ggcagaaccc gcgacgcgac
     6181 gagctttccg atatgcatca aagcgcggcc atgcaattcg cgctggtcag tttggtgggc
     6241 attctggtaa ttgtcggttt catctacacc acactgaatc tgattgaccg ctcgatagtt
     6301 cacattattc cgccaatgct actgcccgca ttggccatgt gtgccttggc catttccgat
     6361 attcgatatc tatggctgga acacgatacc ggcaccggag gcgacgatga agactagtct
     6421 gaaacttcac cgactccaac gcggtatgaa gcaatccgac ctcgccgatc tggtcaacgt
     6481 gcgacgtgag accataggaa ggctggaaca ggggcaatac tgcccatcat tgcgtctggc
     6541 catggatttg gcgcgggtgt tcggcaccac cgtagaggac ctgttcagtt ttgacgacga
     6601 gtacgaatca gaatccgagt agcccgtcgg cgtgtcgcag aaaaaaattt ccctgttgtt
     6661 taccatgcac gtatcgccaa tcaccgcaat cgttggaata gcaacgtttt cagaacgtca
     6721 ggagaccccg ccgcaacggt aatgcggcga caatatgttc gaacgttgtc acatgatgtg
     6781 accacgttgt caccccccct tatccgttaa cataattacg gcgggttcta gtgatcgttg
     6841 caacacctga cctaccgcaa ggagggtcag g
//
LOCUS       DAWBMW010000084         6845 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_2545_length_6845_cov_2.618409, whole genome shotgun sequence.
ACCESSION   DAWBMW010000084 DAWBMW010000000
VERSION     DAWBMW010000084.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 6845)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6845)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6845
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2545_length_6845_cov_2.618409"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1777..1786
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2249..2324
                     /estimated_length=76
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4952..4961
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1416 a         1962 c         2130 g         1241 t
ORIGIN      
        1 ccggcctggc cgaagtccgg ttcggtgccg ccaagggcca ggatggtctg gtcatcgcca
       61 ccacactggg caccggcatc ggcaccgcct tgatttacaa cggcgtgctc atcccgaaca
      121 ccgaactcgg acacatcatc ctgagcgcca agcatctgga cgccgagaag tatgcctcct
      181 ccgcgattcg cgagaatgag gaactgggct acaagaagtg ggccaagcgt ctgaccaagt
      241 actacggcct tatggagaag tacttcaacc cggacctgtt caccgtcggc ggcggcgtga
      301 gccgtcagag cgaaaagttc ctgccgtacg tggatatcaa gaccccgatc gtgcccgcca
      361 agctgcgtaa ccaggccggc atcgtcggtg ccgcctacta tgccagcacc aagcagcagt
      421 gagccagtga gcgtgggtgt gcagcgctga cccgcgaata tgcttcaggc cgtccgacta
      481 gtcgggcggc cttttgcatt gcataggaaa ggtatgcgac gcgttcccgg gaatgttgcg
      541 caaagtgggc gtgtgtatgg aggtctgtgg cgacgcgttc ccgggaatgt tgcgcgtttg
      601 agagcggggc agggggcacc acgctctatc gtgggagggt atgagcgtgc atttctcttc
      661 tcgtgtggat atttctgcgc cgaatcccat tgcggcggcc gaggcggcag ccaaggcaaa
      721 cggcatcgca ctcagcaaac tcaacgattc caatccgacc cgccacgggc tggcacctga
      781 attggcgccg agcgtgtata cggccgaccc gcgtggaccg cgtgcggcgc gagaagcatt
      841 ggccgcattc ctcaatacgg caaaggcggt agacgcaagc agccctcagt cgacttcgcc
      901 cgccgccagc gagggcggga acgagcgcca tgccgctttc aacaatgcga ataccgccat
      961 cgagggcgtt gacccggacg ccctgtactt gctgagctcc acgtccgagg cgtattcgtg
     1021 gctgattaag ttactttgcg atgccggcga cgcggtgctg gcacccaaac ccggctatcc
     1081 gctgattgag tcgattgcac ggcttgaatg cgttgacacg gtcgaatacc agttgcaatt
     1141 cgacggctcc tggttcatcg acatcgccga actcgagcgg ctgctcagcg agcccggtgg
     1201 cgaacgcatc agagcattgg tgctcatcaa cccgaacaat ccgaccggtt cgtacgtaaa
     1261 accaaccgag cgtgcacgta tcgtgacatt gtgccgtgag catggcatcg cccttatcgc
     1321 cgatgaagtg ttcttcgatt actctctcga acctttcccc ggcaacgcac ggctcgcggg
     1381 ggagcgcggc gtgctcacct tcgccctaga cggattctcc aaaatgctgg ccgcgccgca
     1441 cgccaaagtc gggtggattc aggtgtccgg acctgccgag gacgttgccg aggcacagcg
     1501 tcgactggac gtcatcgccg atgattatct gccgatgagt gacatcatcg ccgaacggat
     1561 tcccgcatta ttgaacgccg cgcccgcaca gaccagacgg gtgggggagc gcgtgcgcaa
     1621 taacctggcc aagttgcacg agttgcttga cgccgatccc aacgggctgg tcagcgtgct
     1681 acgggccgaa ggcggctgga acgtgctgct acgagtgcct tcagtcatcg atgagaacga
     1741 gctggtgctg cggttgattg acgagcacaa actgacnnnn nnnnnnaact gaccggacag
     1801 cccggctact tcttcgacat gacttccaat ggatacctgg ccgtttccct gctacccgaa
     1861 ccggacgaat tcgagcgtgg catccgcgcc gtgctcaata ccgtcgccgc tttgctgcga
     1921 taggccggtg tttgccggca ccacgcctgt actccacgta caatagcggc atgggattct
     1981 tgtttagacc gaaagaactg ccacgcccgg cgtcgctgac gttcactttg gatcaggccg
     2041 ggcatacgta tgatgatggg cacgtgggac ttgctcccac ggacatcacc atcagcgagc
     2101 tgcgggtggc tgtgatcgga ctgaacggcg caggcaaatc cacattgctg ggtctgctgg
     2161 acggttcgtt gaaggccaac gccggcacgg tgaccattgc gggcggtgag gaacgtcttg
     2221 acccggctgt gaagaaggac gcgaagcgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaacagc ttttaccaag
     2341 cggcgaatat ttccgaggct gtttccgaag cgctgaaaaa gcacaaggtg cccgagtcag
     2401 aacgtcatgc gcgcatcggc aatctgttcg cgcattttga tttggcgcag gtctccaagg
     2461 ccaaggccag cgaactggac tccgagaaac ggcacctgct ggctattgcg gccgcgctga
     2521 gctttgagcc gagtgcgatt gtggcggatg agccgagcaa gggattggat gagattggcg
     2581 ccgcgcatgt ggccaaggcg ttgttcagct acaacaagca ggtgattttc gccacgcatg
     2641 acacggatat gatccgccgg cccgaatacg ccatcgaccg cgtactggtg ctggacgagc
     2701 acgcggtcgc gtttgacgga gcgccggctg aggctgtggc gttttatgag gatctgattc
     2761 gccgcaagta tgaggctgcg aagccgtgag gtcgattcca atcctcacgc gaagtagaag
     2821 ccgcggaagc cgtactggta gacgcggtag atgtatcccg aactgagcac catcttgtac
     2881 tgtgccttga ggttgccgtc ggtgtcggtg atggtcaggg taatggtgtt cttgaccttg
     2941 gggatcagtc ccaacaccag aaactcgtgc tcggtggagg cgatgtcatt gctcgatacc
     3001 gtggtcgtct gactcgccgt gctggtaccg ccgacagtgc cggaaccgtc ggcgtcgccg
     3061 gcggcggcct cacggtatgc ggcggccgcc tcatcgctgg tgatggcgtt ggccgtgtaa
     3121 tccgggtacc cctcagcgga aacggtgtag ctggaagacg cgactacgga cggcagtgga
     3181 cacggggaaa ccctcacgac agtatgggta aacgattagt gaattacgtc acatacatta
     3241 tgccgcaggt cttggaaaac accggccgga atgctgggag aatactgaag cttggcgggt
     3301 cgccgagcgg tccggtccta tagcagggtg aaccaagact gaacctgatg ccagagcgtc
     3361 ggattgttgg cgatcatcga atcgctgcgc gactgcacga agcaacttag tatggctatg
     3421 cccagcgccc acaatacggc cagcatcacg acccagcgca tcagccaacg ccacggagtg
     3481 acgcgggcga tgccatcgcc tgaagcggcg tcgttggccc ccgccaatga gcggctcggt
     3541 tcgcgcccat tgaccagcca cagtagcccc ggtatggacg ccagcgagat cagccagccg
     3601 aaatcggcgg aataccgcca tcccaaaccg ccgacgcggc tgtcgaatac gaccagcacc
     3661 gccgccacgg ccaagcaaga catcagccat ggccgcatgc cgtgtgtttc caatctgcgc
     3721 agcaggggca gcgccaaagc catcaaggtc agtggcgtgg ccgtgaaaat cgcgccgacc
     3781 atcacctcgt aataacccca ttgcggcatc ggggcgggag agcccgccag ccacgggaaa
     3841 cgatccatga agcgcagcgg caggaacagg taataccaga tgttggcggg catatccgcg
     3901 gacggggtgg tataccgggt catatcggat atggagaact gatacgcatt gccgaaattg
     3961 aacggggaat cgaaccgaac catgttccac gccatcagag gcgtcacgac gagaatggcg
     4021 ggaatgatta ccgccagcgg cgcgcgcaac gcctgcaacg gcttgatgcg gcgtgtgcgc
     4081 agaccggtgc ccagcgcgcg aatctgcggc cagaacaggg ggaacgcaag caatgcggcc
     4141 aaggtgaacg tcggtcggca tccgaaattc gccgcaatgc acaaggcgcc caaggccagt
     4201 cgtggcaggg agagaggctt ggcatcgccc gcctgccatc ggtccgatgg tctcagcgga
     4261 cggcgggacg tgtccgcgcc gagccatagc catagtccca gcgacgtcaa cgtcagcgat
     4321 gcggcgaacg gtatctgata gaagttcgtg cgatacagca aataccccat ttgcgcgctc
     4381 accaacgatg agaccactac taacgacgcg gccgccaccg aagtcttcgg catcacacga
     4441 tggatgacgc gcagcaccag caaggagaag aagatgatga acagcagcac gagaatatac
     4501 tcggcggcgg atgtcggcag attatgaccg gccagcagac gataggggac gaacagcagc
     4561 actgcgggca acacgccaaa ataggaatac cagtgaccgt cataatacgc gtaatcccag
     4621 tagagcggac tggctccatt ggcgagtagc tgggcacgtg tggctacgtc ataggggtgc
     4681 tcggtggcgg ccagctgttc gggaaccggc aagtcgagcc agggccggcc ggcgaccagc
     4741 gcatcggcca catgcccata ctggtcgaaa tcataggtgt aatcgccgga aacgtggaag
     4801 acgagcgaag acgtgtacca cagttcgtgg atgatggata cgccgataag cagggtagga
     4861 atcgccagaa gccccacgaa agcaagtcgt tgccgagtgc tggacgggtc aagcgtgata
     4921 cgccacaggc gcgatcccgg cctccagacg gnnnnnnnnn ncggcaatca tcagcaacac
     4981 caaggccatg gcgagcacgc gcatccaatt gatgacgaac ggtacgcgca cgttggcgcg
     5041 ggcgtcggtg accggtacga tcgcgccgcg ctcttcctga atccaaatac gcacgatgcc
     5101 ggcacccggc gccttgacat aatggctgcg aatagcgtcg ggattcatcg actgggcctt
     5161 gccggtgatg ccatcgacat ccgcgcgcac atgaatggtc gccagcggtt tgaagacttc
     5221 cttatcgtcg ttcgctttgg attcggcctc ggcctgctcg cgggccttat cgatgacctt
     5281 gctgctctcg gtatcgatgc gtaggtaatc cgacgtgccg tccgcggtca gttcaagata
     5341 ggccttggtc ggatcggtga cggtcaacat gccatcattc cggcgcgtga gacccgaacc
     5401 cagcgtgtta tgcacggcgt tcgtatccgt gctcgctccg agcgtacgcc agaacggcag
     5461 gttgaacacg atgcactcca tggcggcgat ggcggcgacg gcggccagca aggcgatcag
     5521 caggcgcacc aaacgcagcc ggtgccgggt atccggacgg ctgggcaggc gccgttcatg
     5581 attcgacggc atcggacgat ctgcgcgagt gtggtctatg gaatgcacgc atttattcta
     5641 taggctttat tcgggcttga tgtcgggcaa acaagagaca atgtgaatca tgccaaaacg
     5701 tacatcggac catgggaagc acaaccgcac gccactgctg ggcggactga aggattcgat
     5761 gaacgcgctc gcggccgatc tgggactgtc caagccgaag aagcacgata cgaatccgtt
     5821 cggcgatgcc ttgcgggaga tcagtgagcg taaaggcggc attatcgacg aactgactcg
     5881 ccatgcagtg gcgctgagtc ctgtaatcaa gcggcgcacc ttccccgaat tggagaacgt
     5941 gccggaaggg ctggtgcttg gatgggcgca gctgccctcc accagaggcc gtggcttttc
     6001 gcttggcatt ttccccgacc gcgatcattt tgcgcaggtg gcgttcgccg atacgcatgg
     6061 ccgcgtattc gtgggcaccg accccaacat ggacgtgcag gatatgcagg cgcgattcat
     6121 gttcggcaag gaaaaggaag tgactctgcc ggacggtgag cggctgggcg aaagcgacga
     6181 gccgcgttca cgttccggtt tctcccgtgc aggccatggc ctgggaagtg cgctgcatca
     6241 tgggcgtggt ttggggcgtg gccttggtca tggtgctgac ggcgtgaagg ccattaccgg
     6301 tggcgtggtg gggcgtgacg acaccggacg catgacgtgg ctcgcctcat ggctcaaggc
     6361 cggcaaccgg tacacgcttg agcagatgcg tgccaatctg ccgcctgtga tgcgtcatga
     6421 tttggaatac cgcgatgcca ttaccatcgc gttcttcgcc acgtatatgt tgccgcagat
     6481 caatggtctg ctcatcggat tcggttccgg caatatcttc cgccgtctca accgtgaggc
     6541 gccaatcggt gcgattcgcc gttcggtgcg tgacactttg gcggcacgag cccacggcat
     6601 gcgggcctcc ggtctggagg atcacttcgc cgatctgatg cgcgaagccg gcgccctcga
     6661 caccacgccg ggactggaag cggtgcatgg cgccgaaccg ttgcacctgt acacctcgac
     6721 gtattcgagc ggctatttct ttacgtggga taagtccttg gcattcgggc aggcgctgaa
     6781 atccttgaag atcgaaggca acctcaaccg attcgctgcg gtgagcgctt ggctggagcg
     6841 caacg
//
LOCUS       DAWBMW010000085         6686 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_2610_length_6686_cov_3.051576, whole genome shotgun sequence.
ACCESSION   DAWBMW010000085 DAWBMW010000000
VERSION     DAWBMW010000085.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 6686)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6686)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6686
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2610_length_6686_cov_3.051576"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    381..390
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1309 a         2102 c         2008 g         1257 t
ORIGIN      
        1 cgcctgcgca ccgtggttga ggcttgggcc ttccgtaccg ccgaaatctc caagatggaa
       61 ggcatcgagc agatcttccc gttcgagaac cacggccagg aaatcggcgt ctccctcgcc
      121 cacccgcacg gccaggtcta ctgctatccg ttcatcgccc cgaagatgga gaaggaactc
      181 cagcacaccg aggcctacca cgagaagacc ggtggcaacc tgcttaagga catcatgaac
      241 gccgagctcg aagccggcga acgcatcgtg atgcgcaacc acagctgggt cgcctacgtg
      301 ccggccgccg cccgttggcc ccttgaagtc cacgtggccc cggtgcgcga cgtgctcacc
      361 ctcgaccagc tcaacgacga nnnnnnnnnn gcctgaacct gcagttcttc tccttccgcc
      421 gtgccgccaa caagatcaag tacctcgccg gctccgaatc cggcatggcc gcctggatct
      481 ccgacaccac gccggaactc atcgccaagc gcttccacga gctcggctcc atcgacatcg
      541 ccgactgata gaaagaaaac atcaatgact gctgttgaat tcattgagcc gctgacccat
      601 gaggaaggcg tttcgcaagc taccaagctg ttcgtcgaca cctacggcgc tgcgcccgag
      661 ggcgtgtggg ctgctccggg ccgcgtgaac ctgatcggtg agcacaccga ttacaacgcc
      721 ggtctgtgcc tgccgattgc tctgccgcac cgcaccttca tcgctctgaa gccgcgcgaa
      781 gacaccaagg ttcgcgtcgt ctccgacgtg gctcccgaca aggttgccga agctgatctc
      841 gatggcctca aggcccatgg cgtggacggc tggtccgcct acccgaccgg cgtggcctgg
      901 gcgctgcgtg aagccggctt cgacaaggtc aagggctttg acgccgcttt cgtctcctgc
      961 gtgccgctgg gctctggcct gagctcctcc gccgccatga cctgctccac cgctctggct
     1021 ctggacgacg tgtacggcct tggttacggc gattccgacg ccggccgcgt gaccctcatc
     1081 aacgctgcca tcaagtccga gaacgagatg gccggtgctt ccaccggtgg tcttgaccag
     1141 agcgcctcca tgcgttgcac cgaaggccac gcgctgctgc tcgactgccg cccggagctc
     1201 accccgctgg aaaacgtctc ccagcaggag ttcgatctcg acgagtacaa cctcgaattg
     1261 cttgtggtcg atacccaggc tccgcaccag ctcaacgatg gtcagtacgc tcagcgtcgc
     1321 gccacctgcg agcaggccgc gaagatcctc ggcgtggcca acctgcgcgt caccgccgac
     1381 ggcatcgcca aggccgacga cccgttccag gcgctcaagg agacgctgga cgcgctgccg
     1441 gacgagacga tgaagaagcg cgtgcgccat gtggtcaccg agatcgagcg cgtgcgctcc
     1501 ttcgtgcgcg ccttcgccaa cggcgacatc gaggccgcgg gccgcctgtt caacgcctct
     1561 catgattcgc tggccgcgga ctacgaggtc actgtgcccg agctcgacgt ggccgtggac
     1621 gtggcccgca agaacggtgc ctacggtgcg cgtatgaccg gcggcggctt cggcggctcc
     1681 atcatcgctc tggtcgacaa gggtcgttcc caggaagtcg ctcagaagat cgccgacgaa
     1741 ttcgaaaagc aaggcttcca cgcaccgcgc gcgcttgccg cgtacgcggc gaaatccgct
     1801 tcacgcgagg catgataata aatcacaatg ctaaatggcg ggtgtcttcc ctatacttgg
     1861 gtagtcaccc gctttttgta tacgaccagg gagagccatg aacaaggcaa tcatcaccgt
     1921 cgtcggtcag gacaccgtcg gcatcatcgc gcgagtctgc acctacctgt cccagcacaa
     1981 cgtcaacgtg ctcgacatct cgcagaccat catcgacggt tacttcaaca tgatgatgat
     2041 tgtcgactac gccaacgcgg acaaggactt cggcgcgatg gtcggcaacc ttgaggacct
     2101 cggcgacgac atcggcgtgc gcatccgctg ccagcgtgag gaaatcttca ccaagatgca
     2161 ccgtatttga ggcggcccgc tcagcaggcc gcctcacgcg aggaggtgga aagcccggtg
     2221 ggctttccac tgacggagcc atccttatcc ctctgatgag ggtgaccgcc aaactagtct
     2281 ttaaatatcc tgtaaggaac aaaccatgct gaacatcatg gaggtccacg agaccaatca
     2341 gatgatcgag caggagaagc tcgacgtccg caccatcacc atgggcgtct cgttgctcga
     2401 ctgcgcctcg gacgatgtcg acaagacctg cgacaacatc taccgcaaga tcaccaccta
     2461 tgccaaggac ctcgtttcca ccggcaaggc catcgaacgc gactacggca tcccgatcgt
     2521 caacaagcgc atcaccgtca ccccgatctc actggtcggt gccagctcct gcaagacgag
     2581 cgaggatttc gtcaagatcg cccacgcgct tgacaaggcg gccaaggaag tcggcgtcga
     2641 cctgatcggc ggctactccg cactcgtttc caagtccatg accccggccg aggagctgct
     2701 catccgctcc ctgccgcagg ctctctccga gaccgacatc gtctgctcgt ccgtgaacgt
     2761 cggctccacc aagaccggca tcgacatgaa cgcggtcgaa ctgctcggcc atatcatcaa
     2821 ggacgttgcc gagcgtaccg ccgacaacga ctcctacggc tgcgtcaagt tcgtggcctt
     2881 ctgcaacgtg cctgacgaca acccgtttat ggccggaggc ttccacggcg tcaccgaggg
     2941 cgacgcggtc atcaacgtcg gtgtctccgg ccccggcgtg gtctcccgcg cactcgatgc
     3001 ggccaagggc aaggacttcg agttcctgtg cgagaccatc aagcgcaccg cgttcaagat
     3061 cacgcgcgtc ggccagcttg tggcccagga agcatcccgc cgcctcggca ttccgttcgg
     3121 catcatcgac ctgtcgctcg ccccgacccc ggctgtgggc gactccgtgg gcgaagtgct
     3181 agagaagatc ggcctcgaac aggtcggcgc tcccggcacc acggcggcgc tcgccatgct
     3241 caacgaccag gtcaagaagg gcggcatcat ggcgtcctcc tacgtgggcg gcctgtccgg
     3301 cgcgttcatc ccggtctccg aagacaagaa catgatcgac gcggcttctt ccgattgcct
     3361 gaccatcgag aagctcgaag ccatgacctg cgtgtgctcg gtgggcctcg acatgatcgc
     3421 cattccgggc gatacctctg cttccaccat ctccggcctg atcgccgacg aggccgccat
     3481 cggcatggtc aaccagaaga ctaccgccgt gcgcgtgatc ccggttgccg gcaagggcgt
     3541 gggcgagatg gcgaacttcg gcggcctgat gggctacgcg cccatcatgc cggtcaacca
     3601 gaccagctgc gaggcctttg tgacccgtgg cggtcgtatt cccgctccga ttcacagctt
     3661 caagaactga ttcagtaggg tatctgcctt caaagaggta catttgcgca gataggtatc
     3721 tgctttcaga ggggtacctc aatcaagaaa ccccgagtac aagcgttgat atttcaacgc
     3781 ctatactcgg ggtttcgtcc tttaacagcc cctcgaagcg gcaatagacc tccggcagat
     3841 gccaaatcag cccctcgaag cgccgataga tccattttct gcgatgatgg tcgtgctgcg
     3901 aggctcaggg tttcctctct cacgcctgtc ggcgttcgtt cggcgtcgct actcgaaggc
     3961 ccaccgggcc ttctcctcag cgccctccag cgaagttgag ttggcgccag gcttcgtaga
     4021 tggcgatgga cgcgcaattg gtgaggttca gggaacgcaa ggacgggcgc atgggcagtc
     4081 gcacttgttc ggccacatga gggccctcca tgatgtccat cgggtcgggg atgtcgccgg
     4141 gctcggggcc gaacagcagg atgtcggtcg gcttgtattc cacctcggtg tacagcttgg
     4201 tagcatgtgc ggtgaacgcg atgatgcgcg aattgggcat ggactccacc aggttgtcga
     4261 aattcgggtg cagcaccacg tgggccatgt cgtggtagtc gaggcctgct cggcgcagct
     4321 tggtgtcgcg caggttgaaa cccaacggct ccaccaaatg caggatggtg ccggtcaccg
     4381 cgcacaggcg gatggccgag ccggtgttgc cgggaatgcg cggtgagtag tagcacagat
     4441 gcggtgttac ggtttcggtg gtggcggcct tttcagccga gagcatcgcg tccaccacgg
     4501 agatggggtt gccatgcgcg tcggttacga gttcgtccgg tccgtaattc gatttacggt
     4561 acccgtactc gaacatcttc tcgacttttg tttcgggatt ctgtgcgttc tgcgaattct
     4621 gttctgcttc gttcgtcata gctgctaaga ataagaacga tgaacgacac agcaatttct
     4681 ttgcgattgg gcgcatggtg ggaggccaat gcgcgcgatt tgccatggcg cttcggccgg
     4741 gccacgccgt ggggcgtgct ggtatccgaa gtcatgagcc agcagacgca gatgagccgt
     4801 gtagtgccgt actggaccga ctggatggaa cgttggcccg acgcccgcgc cctcgccgag
     4861 gcccccaagg ccgaggtgat caccgcatgg gggcggctcg gctatccacg ccgcgcattg
     4921 cggttacagg aatgcgcccg cgtggtggct gaagattacg ccgacaaact gccgcgcacg
     4981 tatgacgaac tcactgcatt gcccggcatc ggcgattaca ccgcctccgc ggtgatgagc
     5041 tttgcgttcg gcgaacgaat tgccgtgatc gataccaaca ttcgccgcgt gctgagccgc
     5101 gtgttcctgg gcgtcgaatc gcgcggggga gcgaccagcc cggccgaacg cgcgctggcg
     5161 aaccgcatgc tgcccaaaga cgagattctc gggtgcgatg ctgacgtcgc cgataatgcc
     5221 ggtagcgccg aacatgtcgt gaacagcact atccgtggcg gcaaacgctc tcgactgcat
     5281 cgtggcgagc gcccatcggt cacgtggaac cagtcggtga tggaactggg cgcggtgatc
     5341 tgcacggcca agtcgccgct gtgcgacacc tgcccgattg cggacgactg tgcatttctc
     5401 aaggccggcc gccccggact gggggagcgc cgcacccgcc cacgccaacg attccaaggc
     5461 acggatcgac aggtgcgcgg tctggtgctc gccgcgctgc gcgagcttcc cgctggagcc
     5521 acgttgccca gggaagatgc cgacaagctt tggaaggacc agattcagct tgccgcctgc
     5581 atcgccagcc ttgacgatga cggcctgata gagattctgg acggcggcct gcgactaccc
     5641 agctgacatt gtgaaaaatg gtgggctccc caagcgtgta tgggcgtggt gaccgtagac
     5701 tggaagagtt atggcagtgc agaggaacac atcaggcgcg agaacggcac gcggagccgg
     5761 caccggggcc aatgggccat ccggcgggcc ctcccaaaac aaccgttccg gcaagccgcg
     5821 caagcgcagg atcagcaaaa agcagcaggc catctatcgt cgtcggcgta tcgtggccgg
     5881 tacggtgctg ctgctgataa tcgcgctgat tgtattctgc gtgtatagcc ttggccgggg
     5941 tatcggtgcc gtgaactcga tgatccacca tgacgaggtg tatacgattt cccgcgacgc
     6001 cacgccggcg ccgaagaaag tcagcaacgt caaggattgc tcggccagtg atgtgacatt
     6061 gcagctttcc gcggcctccc aatccgtgcc ggtaggagga tcgcttgatt tcaccgcgac
     6121 catcgtgcac gaggggtcag gcaactgttt agtggacggg tccgattccg gccgtgtgct
     6181 caccattact tccggtcagg aaacgattta caagtccgat gtgtgcccgg cggattcacg
     6241 tctgctgctg atggctaagg gcgataagga cgttcagaag gtcacgtgga ataccaatgc
     6301 gaacgccacg ctcgccaaat gcacggatga gtcaggttgg gccaaagtca atgcgggaac
     6361 gtacgtggcg cagctgagtt tgaaggatca tccgaaggtg aagagcgatc cggtgacgtt
     6421 tacggtgcag taaaagtctt gccttagcgc tattcttttg actttgcatg aggtgtgtca
     6481 tattgcgggc atgccgtcga caatgaagcg tccgcatagg catatggtgg ccatgccggc
     6541 gctcccaggg gaattaccac atattagcga gcgataagga acgtccattg gctactgagt
     6601 ctacgacgaa caccaccacc atcatcgcac gcgccgacca gcatgacatt gatctgcaca
     6661 aggcgtcgga tcgcgtgaac ttcggt
//
LOCUS       DAWBMW010000086         6560 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_2675_length_6560_cov_2.763566, whole genome shotgun sequence.
ACCESSION   DAWBMW010000086 DAWBMW010000000
VERSION     DAWBMW010000086.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 6560)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6560)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6560
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2675_length_6560_cov_2.763566"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5569..5578
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1300 a         1896 c         1916 g         1438 t
ORIGIN      
        1 ccatggaagg ccgctcaaac ctcaggtgtt gcaaccacca ctagaatccg cctgcgtatc
       61 ggtatcaata taaatctggt tcagaattcg ctgctcggca ttgtcgccgc tcatcaccgt
      121 gccgtcggag agcgtcacgg ggccggcaac cttcaataac cgctgcagga acaccgggtc
      181 cacgctgatg acgccatcca caccgtcatg accggctcga tgccagatct gacgcgcgat
      241 atcagcaacg cgcgggaaat cgggagtgaa attgatgtcc ggatagtctg tggccatctt
      301 ggtggagaag agattgcgtt cttccgccgt cagtacgctc gcggcttcat cctggctgaa
      361 cagaccattc cgaggcggat cgccgaacgt actcatgctg attttgcctg catcgatggt
      421 gagcgtggcc cacgcagtcg gaatgcctcc cgtggcacgc agctcagcat tgttctgtgc
      481 cagcactagg taggttcgcg ggccactgct actgccctcg ctgctatcca agtcgaacat
      541 cttgggcagc acatccgcca gccgactggc cgcatccgtc aggctggcaa gctccttgaa
      601 cttgctacgc ccctgctgca ccgcctcggt aatctgcgga atatgcgtat cgccaatctt
      661 gcccatgtct acactggcat ccgccactac tccattggcg gcggcaagat cctgagccac
      721 gctggccata tcccccagct gaatcgtgcc gtctttgata ccgatgctgt tgaaatccaa
      781 ggcctgtgcc gccttggcaa gcttcggcaa tgcgttgttc gacacgtctt ccaaaatgtg
      841 caccatgtct cgcgtggcct tcacgtcaga accgtaatag ggcaccaatg tggccagttt
      901 ccataccggc tgcttggtct gcgcatacgt agcgtcgata tgctgggaga atgcttcaat
      961 gctggcttgc gctttgtcgg cgtcgccggc ttgaacttgt gattggatgc ggcttgcggc
     1021 ctgaatcgct gcttccactt catttttcgc tttgagcgcg gaaaggccga gccacgccgc
     1081 gagcgcggca atagtcaaca gcagtgcgcc gattacaatc agcaccatcc gaatgcgctt
     1141 tttgcgcttg atccgcctga tatgttcatg gctcatacgc cggcggtgct tacgccgttc
     1201 cgccgctcgg gcgtcttcag caagtacctc ggggtcaaca tacggctgta tcacttggcg
     1261 atcatccgcg ttttccgacc gctcggaggg tgccggcggc tgaggtattt ctggtgtgtt
     1321 cgaatcagtc atagatatat cgacttatct ctctggaatc acgttcaagg gcggggcggc
     1381 aagggcgcgg caagactgaa gcagcgaaac ggaaaggaat cagtacgctc ccttgtgact
     1441 cagcacggca ccgaccgtgc ggaagagaag cacaatgtct cccataatcg accagttctg
     1501 cacgtaagcc acatcaaggg cctcactctc ttcggcagac aaatcggagc gaccggacac
     1561 ttgccacgga ccggtgatgc cgggcttgac cagcatgcgg gtggcgtaca gacggttgta
     1621 acgggcgtat tcctcaggca gcggcgggcg cggccccact acggacatat cgcccatcca
     1681 cacgttgagg aactgcggca actcgtcaat ggagaagcgg cgaatgaaat gccccacctt
     1741 ggtgatgcgt gggtcgttct tcatcttgaa gatgaagcgg tcggtctgac cggtttcttt
     1801 tgcgagctcc ttcttgagcg cgtcggcgtt caccaccatc gaacggaact tgtacatctg
     1861 gaacggcttg ccgcgcaggc ccatacgggt ctgcttgtag aacaccggtc cgccatccgt
     1921 gactttgatg gcgatcgcca ccggaatggt gataatcaac gaacagatga tcgccagtga
     1981 agaaatcacc aaatcaaaca agcgtttggt caaacgacgg gctggcgtgt attgcgcaag
     2041 acgctgcgtg ataatcgtgg tgccctgtat ggatcgaacc tgaatctcat ggttagaagt
     2101 gtcagcggcg gaagccagca tggcaatctc cagaccgaac gattccatgc gcacggagaa
     2161 gatatcgtaa ttatccgaat agcgacgcag ctcgtcagcc accataacgg tctgcgcgtt
     2221 catgtcaata atctgttcag caagattatg atcgctgtac tcgagtacgg gcagctggca
     2281 gcccttgttc ttttgcatct cctcacggag tgtctcatgg tcggcgctct gttcaatcaa
     2341 tccggtatcg ggattgagat gaatcgggca tactgccacc gggcggtagt tgagttgccg
     2401 acgctgaccg aggaattgta gcgtgcgacc aatgccgtgc ggggaaccga tgatcaccgt
     2461 gccataagcg taggcgccct tgcgacgact acgcgtaatg aatgcacggc tggcaatgcg
     2521 ttccaccatg gtgagcaacc atccggcaat caccaccaag cagagcgacg tgagggtcag
     2581 acccagattg aagaagtagt tgaacgcgca ctgcaccacc cagcacacga ccatgccttt
     2641 gaccagcagc acgttgagct ggtaaccgtc acccataaca tggcggtgat aaatgccggc
     2701 aacatagagc gaccacaccc agaagcaggc cacaataata aggtaaacgg tggagtcaat
     2761 cgtgaaattg aatctgctcg aatagagcgg cgttgcctca tggcgcagat tgagcaccgt
     2821 ggctccggcg atgatgtaca tggcggcgtc gataagcatg agggcgagat tgacgaagaa
     2881 tcgccagtac agcacgtggc tgagctgctt ttggcggata tctctgagct gagcggtggt
     2941 tcggtcaccg ctattggcct gtctgctgcg tgtagacggc tgaccgttac tgctcagata
     3001 cgacacctca gacaccagtc cttccttttc gaccgtatac tgcaggtctc gcggctctca
     3061 cttctctgga ttcccccgtg tacgggtgta tacaggggtc accccttcaa catagtacag
     3121 acatcacacc cctaaccgct aggggggggg gtcagaaaac cacatagcga acgtgatcgg
     3181 atagtaacta cgttcatttt gacgtctcga ataacgggac taatttgctc tgcaaccggg
     3241 cggccagtca tctcggcatt gcatctcgac ggactacccc tgggtgtagc gtgttggggt
     3301 tgctttgaga gaccgtctga tgcgttgttg cggtcgatca gcgagagtgg agtatgcacg
     3361 tgagtggaat gaacggagct cagggaatca agaatcatcg cggcgaactg cagcgggtgc
     3421 tgagccgcga ggaatcatat gtagacggca aactcagccg ggcggcgttc atctccatcg
     3481 cgatattgac ctttattacc ttcgtgggca acttcacgca gctgcagctg agttccgcgc
     3541 tgccgattat cgtttccgaa ttccacatct ccgtaaccac cgggcagtgg ctcacctccg
     3601 tgttccagct ggtcatgggc gtgatggtgc cgctgaccgc gtttctgacc cgacgcttct
     3661 ccacccgcca gatcgtcatc tgctcgatgg cggtgttcac ggtcggctcc ctgctggcat
     3721 ggttggggcc aaacttcgta tgggtgctgg tcgggcgcgt gctcgaagct gccggcacag
     3781 gcgtgatgtg gccggtgctg caaatcaccg tgttctccat ttacccgctg tcccgacgcg
     3841 gctttgctat gggcacggtg ggcatggcca tgagcgtggc tccggcaatc ggccccacct
     3901 tgggcggctg gcagaccgat gccaacggct ggcgttcaat cttcctgacc atgaccgtga
     3961 ttggcgtggt ctcgctgctg gcggccatgt tcggactgca taatttcggt tcgcatgacc
     4021 cgtctgcccg cgcggacttc ttctcggcat gcctgtcggt gatcggcttt ggcgggctta
     4081 tgttcggctt caccaattca gagacctatg gattcgcggc accggtcacg tgggggccga
     4141 tggttgtggg gctggtcggc atcgtgtggt ttgtgatgcg caacctgcgc gctggcaagc
     4201 ggtatcgcga ggccgtagcc aaggacgagt cggcactccc ccagccgccg ctgctggatc
     4261 ttgaagtact gaagaatcgg tcctttaccg tcggcaccat cacggcatct ctggcgttct
     4321 ttgcattcag ttcgattctg gtcatcatgc cgctctacat ccagactgac cgcggctatt
     4381 cggccacgat gagcggtctg atcatgctgc cgggcgctat cggccagtgc gtctcccagt
     4441 tcttcggcgg ccgcgcgatg gatcggttcg gcgcgcgccc ggtggctttg ttcggttcga
     4501 ttgtgttgac ccttggcacc ttgggcatgt cgctggtggc gatggatacg tggatctggt
     4561 gggtatcgat ctgccagttc atccgtcaga tcggcatggg tttcctgctg atgccgatta
     4621 ccacgtggtc cctgaattgc ctgaacgggc cgagcgaagt gtccgccgga tcatcggtga
     4681 cgaataccgc gcgtcagatt gcgggcgctg tgggcgcacc cgtgttggtg attctgatgg
     4741 aaacgtttgc cgctttgcat aagcagggcg gcgcctccgc cgtcgccgct tcgattttcg
     4801 gcattcaatg ggcgctgcgc atctccgcaa taatctgtct ggcaatggtg ttgatggtgt
     4861 tcttcggcgt caagggcgac ggtgccggtc gtacgcgaga cattatttcc ttgcgcgcgc
     4921 tgcgcgaacg gcgtgcgcgg cgtatggctg cgaactgacg gcagattcaa ccattctcaa
     4981 cataataaga aaccccggtt ccaacaccgg ggcttcttat tatgttgtgc tatcgcagga
     5041 tttcagctgg agtctattac gccctgccca tcagcgtcag gacaatcagc agcacgttga
     5101 gcgccacaat cagtgccacc acaatcataa aaatcacgtg cttgacggtt ccatcagccc
     5161 acttgcccat gagttccttg tcatgcgtgt atcgcatcag cggaatcacg gcgaacggga
     5221 tgccgatgga aagcaccacc tggccgatga ccagcgcctg agtcgggtcc ttggcgaacc
     5281 acagcacaat cagtgccggc accagcgtga ccacgcggca ggcccacatc ggggcgttga
     5341 cgtgcagcag accgtgcatg atttccgagc cggcatacgt gccgaccgat gtggagctca
     5401 gcgacgaagc cagcaggccg atggagaaga tcgtgccgat caccgggcca agcacctgcg
     5461 cgatcgcccg ctgtgcgccg tcgatggaat ccgtgccaga catgccgtgc agcgagttgg
     5521 cggcgagcac cagcatcgcc agattcacgg tgccggccag caacagcgnn nnnnnnnnga
     5581 ccacatcgat tttcgagcct ttcagctgcg ttttgatcga cggcttttcg ccgcctgcgt
     5641 aatggtcgtt caccagcgtg gagtgcaggt agatggcgtg cggcatcacg gtggcgccca
     5701 gaatcgaggc ggccatcagc accgagtcgg tgccctggaa gcgtggaatc atgcccttga
     5761 ccacttcgcc ggggctcggc gggtccacga acaggcctgc gatgaagccg aatgtgataa
     5821 tcagcagcat cacgatgatg atgcgctcga acgtggtttg cgtcttgcca ccttggaacc
     5881 acagcatcac cgtggagatt gtgccaatca ccaaaccgcc gatgaacagc ggcaatccga
     5941 acagcaggtt cagggcgatg gcaccgccga tcacctcggc cagatcggtg gcgatggcga
     6001 tgacttcagc ctgcataaag aacatgaatc ggccggcgtc gctcatacgc tcacccagca
     6061 gttccggcaa ggatttgttg gtcacgatgc cgagtttggc ggactgatac tggataagca
     6121 cgctcatggc attggcgagc accagcaccc acaccaacag gtagccgtat cgcgcgccgg
     6181 aggtgatgtt ggccgccacg ttgccaggat cgacgtaggc cacggccgcc acgaacgcgg
     6241 gaccaaggat actggccagc gcatggtcat gcggcttttt cttgccgttt gccacggttc
     6301 cagctccggc tccgactccg gcaagctggc gttccttctc gatttccggt gtttcctgtt
     6361 cgatgtctgc cacggcgttc gtcatagtgg ttgccgcaat ggcatctgct gcggtgtgtg
     6421 ttactgccat atcgtctgtt cctattccct tctcccttca taccgggctc agttatagcg
     6481 cgggcatgtt tcccgagtca acagacggac aatctgcgga cgattgatgg atgaccggcg
     6541 gactggcagc tggcatctcc
//
LOCUS       DAWBMW010000087         6357 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_2777_length_6357_cov_2.687083, whole genome shotgun sequence.
ACCESSION   DAWBMW010000087 DAWBMW010000000
VERSION     DAWBMW010000087.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 6357)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6357)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6357
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2777_length_6357_cov_2.687083"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1155 a         1895 c         2023 g         1284 t
ORIGIN      
        1 cgatgacgta ccagtcgtga gtcagatcag ctggcttcgg agtgaaagtt ttcactggat
       61 taccttttct atattgtgtt ccgggtctca gctgcttggt gtggcttggt cagggccgca
      121 tccaagaggc gtcgaacctc attatccgtg ggctccctga aagtcctcga tggcgagtgg
      181 gatagcgctt tgaaggaccc cttagggaaa cacaacaatc cactagtata tcgccgggct
      241 tgacataggg caactgggga cactccattg ggtttatggc ttctcttccc ctatttatgg
      301 tttctctccc acccacttcg gctaaccgac tcacctacgg tgaggcaatt ccctgccttc
      361 tctcagctat cgcttctttc aggcctcgtc tacggacagt ccactggact gtccgcttaa
      421 cgactcggct cgtcagaggg aggtaaagcg ggtcaggaat tgaagatttg ggcgaagcgt
      481 tgggcaccgg gggtgtcgaa gagggtttcg agcggtacca cgttgccctt ggcgtcggcc
      541 agtgggatgc gccagttcgg gtattcgttg ttggtgcccg gctggttctg cgcacgcttc
      601 tcccccaccg cgtcggtgat cgaggcggcg agcagcttgc acggcgaacc cttcagtgcg
      661 tggtacagcg cgtcgacgat atccgcctcg tgatcctcac gatgttcggc aaagtcggca
      721 tcaagataac cctgctcgac cagcatggcc agcatcgcat cctgctcggc cttggcggag
      781 gcttcgaatt cctcggccgg gccggtcagc aagcccagac gctcacgaat cttgacgtgc
      841 tcgtattcca ggtaaccggc ggccggcggc aaatcatgcg tattcacgga ggccaatgca
      901 tacggacgcc actgggaggg ggcgcggaac acgccgtcgc actgctcgaa ccattcaacg
      961 gcgcaaccga gaataccgtg gctcgacagg gaatcggcca catggtccgg caccacgccg
     1021 aggtcctcac ctacgaccac gccgccggcg cgagaggcct cgatggccag aacgcccagc
     1081 atgatgtctg agtcgtagta cacgtaagcg ccatccatag ccgaacggtt ctcgggaatc
     1141 caccacaggc ggaacagacc aaggatgtga tcgatgcgca ccgcaccggc acgggcgaac
     1201 atgccatgca ccatattgcg gtaggcctcg tagccggtgg tctccagatt gatcgggctc
     1261 agcggcggct ggctccagtt ctggccttgc tggttgaaca tgtccggcgg tgcgccgacc
     1321 gtggctccct tggcgaagcg ctcgggattc caccacacgt cagcgccgga ggggtgcacg
     1381 ccgacggcca tatcagacat gatgccgata tgcatgccgg cgtccttggc tgcctgttgg
     1441 gcggaggaca gctgctcggc ggcaatccat tccagccagc ggtagaagtc caaagtgtcc
     1501 gggtactgct tgcgcagatt ggcgatttcc ggggagttgc gattgaactt gcgctcccag
     1561 ttgctctcct cgccgttggg tgccccccac ttgtcgtagc acaggcacca cgtggcatac
     1621 gcctccaagt cggaaccaca gtccgccttg aattggtcga atacgctttg gcgttcggcg
     1681 gtacggccgg ccttaaagat aatccacagg gcgtgcatct tgctgcgcca catggcgtca
     1741 cggtcgatga gctgggaatc accgttcagc ggttcggtgt cggcatgcag tccgtcgacg
     1801 gcattcttgt cgccctcgct gagcgtggca tattcggcga tcgcctcagg acggatgtac
     1861 gtgaagttga tcaggcgacg gcttaccggc agatacggcg acggggtgag cggggacacc
     1921 ggctcggcgg catgcaccgg attaatgagc acgaagtcgg caccggtctt ctgcttggcg
     1981 tccaccagca tggtcttcag atcctcgtaa tcgcccacgc cccaggagtt ggaggagcga
     2041 atcgagtaca gctgggccat ccagccccac agactgccgc ccttcatcgg gtcaagcaga
     2101 tcgacgcgat caggtgcact gatcaaagtg gcgtcttgca cacggtcggc gaccttgacg
     2161 tgcagcgtat ggtaaccaat cggcagatcg gcgggaatga ccacagccgc attcgaaatg
     2221 aacgtaccat ccaagtcgta ggcctgggat ccatctccag gaccaacttc aatggtgcct
     2281 tgatatggct cgccattctc cagcgtgatg ctcgccgaag gcacgtccag aataccggtg
     2341 ttcaccagca cccggtcctc gctaccggcg atatgcagca cggtgggtgc caccaatcgg
     2401 gcgtaacgct cgttcagaat gcgtcggatg gcgatgagtt gggcgctctc gctggaggcg
     2461 tcgatgccca gcgctgccag cacctcgatc aggacgtcat cgtctatctc gtggtagtca
     2521 tggctcatgc ccaaataact cgtgccgaca ccgaccaact tggccagtcg aatcaacgga
     2581 cgggccagac gctcttcact ctcggtaatc tcactcatag ccccagtcta acgccgtttg
     2641 attgcgccac accgtaagaa aaatgccatg attccgccaa attatgtact ttttaccctc
     2701 acatagtgag atgtgagcgc tcacaatggt ggacaggggg tgattgccgg ccaaatttgc
     2761 gcggcaaaac acagcaatag tgtgcttagc ggcggctttc cacggcgtgc cgcacctcgg
     2821 ccgaggggtc gttggccaat ttggcaagca tcgcttcgga cgtattgggg ttcagtgcca
     2881 cggcacggcg gaccatcgaa gaaagcaccg ccggtgctcc ctcttcggat tcggtgccaa
     2941 gtacgcccaa gaaatccaat gtctccgcat ccgcagtggg gtcctgcgca ccctcaagcc
     3001 gcatcttcca gctggtgcgc ttgtttttca acgccgtatc ccggaccgca ggcaccgggt
     3061 ccttggtcag acggccgacc agccaattct tgtcgtcccc gttggcggcc acggcttgac
     3121 gcacggacgg ttcggggtct tcggagagtt tgaccaatac gttcgggaac ggcatggtgt
     3181 cggcgaggaa cacacgatcg gccaccgggg tgtgcgggtt gccggccaca gcctccaaaa
     3241 gtgcggtggc gcgggagaac gccgcctgat cggcacggtc aggcaacggc cgacgggcga
     3301 attcggacag ttcggccggg tcggtggaat ggcgcagacg ctcgtatgta gcggtcagcg
     3361 gttcgaaatc caccggagcg gcggactcgg ctgcggactg cgcgccttgg tcctgctgct
     3421 gggcgtccgt gtcggttgca gtctcggccg cggcctcggt tgcggcctcg gtgggctggg
     3481 tctgctggct ggtggtgttc tcgtcggtca tgatgcccag cttagtcata caggcgtcac
     3541 gcgagcggaa ccggaatgtt gaccatgtca atcggagccg gcaccacagc ggcattgaac
     3601 gcttcccgca cacgctcctc gaatcgcgcg caatcatcct gcggcaccac cgcggtcaac
     3661 tcgacgttca ccgcatacga ttcatcctcc tgactgccac caagctgcgc caggatctgc
     3721 tggaatcggg ccaactgcgg gtattcgagg cgaacccggt atcgcttggc ggtcatgatg
     3781 cgggcggcgc gggcggcttt catcgccaat gatgcggcgg atgagtacgc gcggaccagc
     3841 ccgccggagc ccagcagaat accaccgaaa tatctggtga cggcaaccac gcaatcggtg
     3901 gtgcccgact gcttgagtac gtcgagaatc ggcttgccgg cggtaccgga cggctcgccg
     3961 tcatcgctca tgcgttcgga cagtcgccca tccgcgcctc cgcacacggc ggcatacgcc
     4021 acgtgtcggg ctttcggatg ctgctcacgg atcgcagcca cgaacgcgag cgcctcgtcg
     4081 agcgtatcga catgggcggc gtcgccgata aattcggact ttttttcgac gagcgaatcg
     4141 tgggcaggat cgtcgggcgt gttgagaatc gtctgcatga gcgtgttccg gtggtcgact
     4201 ggctgggtgt tcgattgagc gcgaggctag gcagggctat cagcgttcgg agacgacgac
     4261 gttatagtct tcgccggcga agtaggcggt caggcaggcg atcatctggc gggtgagctc
     4321 cttgaacgcg ccgtcggcac gccagccgac gtgcggggtc aggacgatgc cgggcacatg
     4381 gcagatggcg tcgtcggcgg tcagcggctc atggtcgaac acgtcgagtg cggcgggaac
     4441 atcgcccttt tgcagtcggg caagcagcgc accggattcg atgacttcgg agcgggcggt
     4501 gttgatgaac agggtgccgg gcttcacatg cgcaaggttc tcggcggtga cgatgccgcg
     4561 cgtggcgttg ttcagcggca gatggacgga gatgacgtcg ctggtttcga tgagcttgtt
     4621 gagatcgtcg accggagtgg caccggagcg ttcgaaatgc tcgggcggca catgcgaatt
     4681 ccatgcggcg acctccatgc cgagggcgcg ggcgatgccg gccacggtgg agccgatgcc
     4741 gcccagtccg atgatgccca tgcgacggcc ggcgagttgc agtccatcgc cgctggccca
     4801 cgcccagttg ccggccttga cgtccttatc gagttggcca acgcggcgga tgagctcgaa
     4861 cagaagggcg aaggtgtgct cggccacggc gtgatcgcca taatgttcgg cgttgcagac
     4921 gcggatcttc agctcgcgcg ccttatcgag attgaggtag ctggcgacgc cggtaccgca
     4981 gaacacgatg cacttgacgt gcccctgccc gctgatggtg cgcagcagat cgtccgaaat
     5041 gtggtagctg cccaccagca gggcgtcggc atccttcaga cgctcggcta tggtcgcctc
     5101 gtcggacgtg aattcctcga acatacgcac gcgggcaacg tcgtggagcg cggggaactg
     5161 ggccttgatg ggctcgatca tcgagtcaag cacggacggc atcacgacga ggggcagatc
     5221 ggtgcgttcg ctttgggttt cgtaaatctc ggtcatgggc tccacattag gaggccggcg
     5281 ggaaaagcgt caccttgcgg cttcccctcc ttgagaccaa gccgagcaac tattgctttg
     5341 ccgcctgctc ctgcgtgatg ttccattcct tgacgacgtt ctggtccgcg cccatcgaat
     5401 agatgacatc ccagatgcgt tggcgtatct gagcgtcgta ggcttcgtcc caacatgtgc
     5461 ctatgggatg gtcgctggat atcgggcacc attggtagtt gccttgatgc acgttggcgg
     5521 tgggcaccca atcgatgcgg ttgacgcgcc acgacccctt gacgcccttc ttgccggcga
     5581 actggatgcg tgcggtcaca ccctgattgt tgaccaaggt ttccggcgtg ttcgccgact
     5641 cggtgacggc gttgcccaga ccgtagataa tccacgtgcc gttatagttc tcgatgggct
     5701 gcgcacaatg gcaaccggca ccgtaaataa cgtcgaaggc accagtgtcg gcaagctcat
     5761 gggcttccga aatctgccag gaatcggcgt aatcaaggta ttcctgcacc gagtgcatgg
     5821 cgatggccac cacatcggca ccttgctcgc gggccttgtt ggccttgtct accgcgcgct
     5881 ggatgtcgct gtcgtgattc gggtcgccgt cttcgcgcag acgatccacc tgccagtcct
     5941 cgtcggcggt catgccgttg agggagaccg tgccggtgac cagacccagc ttgccgccgc
     6001 cggtcgggga atcgatgacc aacggtttgg tggagtcggc ttcggttttg tacgagccgg
     6061 tctgggcgat gccattggcc tcaagcgtgc cccacagacg ggcgatgcca tccgccccct
     6121 gatcccagga atggttggtg gcgtgtgtgc aggcgttgta tcccacgttg cgggcggcat
     6181 ccgccacttc cggcggaatg ttgaagatcg ggtacgcggc atatgggccg ccgcgctggg
     6241 cgatcggggt ttcgaattcg cacacggcga tgtccgaagc ctgaatgtac tgcttcatcg
     6301 gcgcgaacag cgggtcgaag ttgaaggcgg tgccatccgt ggcgttgggg ttcgggc
//
LOCUS       DAWBMW010000088         6281 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_2815_length_6281_cov_3.473338, whole genome shotgun sequence.
ACCESSION   DAWBMW010000088 DAWBMW010000000
VERSION     DAWBMW010000088.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 6281)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6281)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6281
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2815_length_6281_cov_3.473338"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1243 a         1840 c         1910 g         1288 t
ORIGIN      
        1 agataatagt ggccgcgatc agcaggtaga ccagcggatc ttggaattgg gccaggaatt
       61 tcttccattt cggtacgggc ggggcgctgg cgagctcgtt cgggccgaat ttggccagtc
      121 ggcgcttggc ttcttcctcg ctcaggccgt gggatgggtc gacgttcagg gctttggcca
      181 cgtcgtcggc gctggtcaga cttggatcag tatcccccaa tagcgcttcc tgtgcgcgca
      241 cctcttccga ttccacgacc ccgttcgttg cggtcgtgga cactgcggat tgatctttga
      301 gatcatccat aatgttctcc ttggtattgc cgcggagtag tcagccttgc ggccgctgcc
      361 gggttttctc tcccggctgg acgcacagga ctgcgcggtt atggacaccg gcgcaactgc
      421 tgccgatgca tacgattgcc attattccat aaccgcgtgg cgcacttggc gcacagcgta
      481 atagcgtttt gcctagaaca ggcgacgcaa ccaggcctca gccaaatcag gccaacactg
      541 cacgcattcg acgatgtgcg catagaagcc gttaccggca gtttccgcat tggccaagga
      601 caggccgtgg ctgccttcag ggaacaggtg cgcctcaatg ctcacgccgg cggcccggca
      661 ggcctggatg agcattagcg tgttctccac gggcacggcg tcgtcggtca tggtatgcca
      721 gacgaagacg ggtggcgttt tggcatcgat gtgcttttcg atggagaact tgtccagcaa
      781 agcctgattg tgagcttggt cccctagcag gcattggaag ctgccgcgat gggcgtactt
      841 gccggcggtg attactgggt acgagagcat cagcgcgttc gggcgcacgg cgtccgggtc
      901 tatgccgccc tgttcgcgga tgtcttcgtc gccgaccgag gtggcgaggt tggcggccag
      961 atgcccgcct gcggaaaagc cgagtacggc cacttgcgcg gggtttacat gccactgatc
     1021 cgcattggcg cgaatcaggc gcattgcctc ggcagcctcc agcaaagcca ccggatagcg
     1081 gctgggctgc accgaatagc gcagcacgaa tacggcgaag cctttggcca ggaattgcag
     1141 ggccactggc tcggcttcgc ggtcagaagt catggcgtag ccgccgccag gcaaaatcag
     1201 gatggccggg cgtatacggt ccggttccat ctcctcgctg ttgtcggcca catagccaaa
     1261 gaatcgcgcc tcgctgccat caatgccaac gatggtttgt tgaatgtatt gcatgccata
     1321 catcatacgc gcagagaata gttctcgata agaaccactc gccatatgcg gtcttgcccg
     1381 tatctccaaa ccgaataaca cttacagcga atcgtgaaga ctgcgggcca tggcgcggtc
     1441 gcgcgagcct ttggtgagct tgagaccgag ggtcacggca atcaacacaa cagtgcaggc
     1501 gatccacagc gttcgaccgc cgaagttgct gatagtccag ccaccaatca gcggcgccag
     1561 cgcaaccgac accgaccaca ctacgttgta gacgccctga tacgtgccac gcgccgacgc
     1621 gggtgccatg gcggcaaccg tagtggtggc gatagggaat gtgcccagct cacccagcgt
     1681 ccacagcact acggcaagcg cgaatccgcc ccaagaatgg gccgcggcct gcaccgcata
     1741 gccgatgacg gttacggcga ggcccatcac cagcacgcgt gaattgccca tgcgcgcgaa
     1801 cagtttcatc gcgggaatct gtagcaggca caacataccg ccattgatgg tgagcagcac
     1861 cgaatactcg cccagcccta gaccaaggtc cgtcatggca atgggcaggc cggaggtgct
     1921 ttggaagtac acaaggaagt agccgaacat cagcagggag aagccgagga atggagtatc
     1981 ggtgagcacg cgccgccaat tgcgagacat ggcgcccttg acactggcag ccggtgcggc
     2041 atccgagtga ttcggcctct caatgccggc gaattccgca tgggtaacac tgcattcgtc
     2101 ttcatggcgc accgcacttg gcaccgatac agtaatcccc aaatgccgca cctcgcggaa
     2161 gaacgcaatc agcaatatcg tgacggctat catcaccgcc gcttcaacgt agaacatcag
     2221 cgaatacgag atcttgacca gctggttggc cacaatcggt ccaatcgcgt acccaaaatt
     2281 aatcgcccac gattgcagta cgtatgcacg ttgctgacgt tcggcaggca ccacatccgc
     2341 gatatacgcg gcgatggccg ggctgggcag cgaggcgaac gcaccataga cgaacagagc
     2401 cgcgcctaaa gacacaggat cagtgagtac ggaaacgacc agcagggcag ccgctgcgcc
     2461 gatttcgccg atgataatca tggattggcg gccgaatctg tcggacaagg taccgccgaa
     2521 caggcagccg atgattgcgc cgaagccgta catggccacg actgtgccca ccacaccctc
     2581 atcgaggcgc agcgcggaaa ccaaatatat cgacaagaat gaggtcacga agcgacccat
     2641 ccacagaatc agaatcgcca accataatcc ccaatacagc agggacagtc cgaatatcat
     2701 acgctgcttc ttcactctca ataccttacc ggctaccgac ggcggaatag ccgcgttttg
     2761 gcgcggggtg ctgggacaat agcacctatg gctatcaaaa aaggaccgac caaggggtcg
     2821 ggtggcaagc atcgcaacaa actgaagggc tacggcccca ctcccaaggc cgaggaccgc
     2881 gtctatcaca aggcgtacaa ggccaagaaa gcggccgaac gtcggcagat ggccgacccg
     2941 cgtctggcag cccgccgtcg agtggacaag ttcgcttcgg cggatacttc ggatctggtg
     3001 tacggccgta attccgtgct ggaagcactg cgcgtaggcg taccttcttc cacgctgtac
     3061 atcatgtctc gtattgaaca tgacgatcgt actcgcgaaa tcgtgaaaat cgctggtatg
     3121 aacggcctgc acatgctgga ggccgatcgt ctggagatgg atcgtatcgc ccgctctggc
     3181 aaccatcagg gcgtgattct gaaggtcgat ccgttccagt actcctcgct gaacgagctg
     3241 gccgatcgtg ctgagaagaa ggccaaggca atggaggccg cgaattcggc ggccgcccgt
     3301 attgctgcgc gaccgctgtt tatcgcattg gacggcgtga ccgatccgca gaacctgggt
     3361 gccgtgattc gttcggcggc cgcattcggt gccaatggtg tgattctgcc ggaacgccgt
     3421 tccgcttcag tgaacgccgc cgcttggaag gtgtctgccg gtgcggccgc gcatatgccg
     3481 gtggcgcgcg tggtgaacct gactaaggca cttgagggct tgaaggagcg tggatactac
     3541 gttgtcggcc ttgacggtgg cggcgataag ctcgtcggcg agactggctt tgaatccgat
     3601 ccgctcgttg tagtgctcgg ttcggaaggc agcggtttgg gccgactggt gcgcgaaacc
     3661 tgcgacgcga ttgccggcat cccgatcagc tcgtctgttg agtcgctgaa cgcttcagtg
     3721 gcggcgggta tcagcctgta cgcggtgtcc aacgcacggc gcaaggctgc ggcggagtga
     3781 ttgtcgcttg aggccgtaat aggttgtaca tacacggaag cccctctatt tgaggggctt
     3841 ctttgtgtga tgggctcacc tacagacagt ccaccggatt gtccgcttaa tgactccaca
     3901 ctgccgcatc cggatcgtca tcggggttgt agccgtcgtc gtccgggtcg ctgtaggtgg
     3961 attcgtcgtc aatgacgccg gaatcggcct cttcgttaag ttcggcgagt gccttggagt
     4021 caaggctgta gctcggcaga gcgttgtcga tgtagctggt cacggcctcg cccatcggca
     4081 cgtcgtggcc ggccttctcc gccaggaacc agcgatggtc aagcacctca tggaagaact
     4141 gagcaggctc gatctgggag cgatattcgc gcggaatcat acgcacggtc ggctcgaaca
     4201 cctcgcgcat ccagtcggtg gccacgattt ccagatcctc gccctcacgc cacgtggagg
     4261 cacggtaggc gtcaaggtcg ttgagcagac gacgggcctg gttctcctgc acgtcaaggc
     4321 cggtgaggcg cagcagcttg cggttggcat agccggcgtc caccacgcgc gggcggacca
     4381 gcacacgctt gccgtcctcg gcggtcttca tttccagctc gtccacgtcg aagcccagat
     4441 cgttgagctt gttgacgcgc tgctcgatct tccacatttc gtccgggttg aacttgtcgg
     4501 tgtcggtcag ggcactccac agcgagtggt agcgatcaac caggcggttg cccacctcga
     4561 tctcatccac gtcgccgggc agtagcttgc cggacgccaa atccataagt tcgccgatga
     4621 tgttggtgcg ggccaagtcg atatcgtatt cgcgctggcc gtcggtgagc tgggcttgca
     4681 ggtcaccggt ctcggcgtcc accaggaagg cggagaaagc gtcggcgtcg cgcaggaaca
     4741 ggacgttcga cagggacaca tcgccccagt agaagccggc caggtgcagg cgcaccagca
     4801 gcacggcgag ggcgtcaatc agtcgttcgg cggtgtccgg gcgcaggttg cgggcgaaca
     4861 acgcacggta cggcagcgag aacttcaagt gccgggtgac cagaatcgcc tccagcggct
     4921 ccccttcacg ggtgtgacgg ccgatgacca cagcgatcgg ggtcacagtt ggcagttcga
     4981 gcttctgcag tcgacgcagg atctcgtact ctcgctcggc cacctggcgc gtgatctcct
     5041 tcatggcgta cacctcgtcg cccacgtgca cgaatcgcac gacgtggcgg gagatgcctc
     5101 gcggcaggtt ggccagcagg tccttaggcc aggtcgccaa gggcttgtgc cagggcagcg
     5161 tgaacatctt tgggttcgag ctggcggcgg tgattttcag tgcttgcggt tcggctgaat
     5221 tggcgtgttc gtcttcggct ttgacagacg tggccttcag cgcacgcgga tccatctggg
     5281 gcaattcgtt caactccatg tgttcgattt tacgtgtcgt tcgcgatgtg tgctcctttg
     5341 ccacgacttc ggcgcgttat gtttacggac gattcaactc gccccatgcc gctcattgca
     5401 tatcgttgga cttggtttgc tgtgcaagct gcaaacgcag ctcatcgatc tgccgctgca
     5461 ggctctcgat ataacgtgtt tgcttgcaga tgatcagatc gcgcacgtca cgcacatcga
     5521 ccttgctgac ttctctgtct tgctccgtgt ccatatccac agaaaacagg cgttccgggc
     5581 tgcttgccac ttccagatct tccagctgca ttttcttccg ccatcggtcc atgcagcgtt
     5641 caatacgttt ttcgcctatg agctcaggcc ctagtcccgc ctcacggaac aggtacgtgg
     5701 gcgattcgcc ggcatcggcg cgcatcacgc attcccgttt gaaccactcg gcatacctga
     5761 tgcgtgatgg cgataccttc accaccgccc gcagcttgat cagacgtttt atctgtgcgg
     5821 atgagaacga ggccttgcta tgcgtcatgc ttttcctccg tcggcacgga actggtgtga
     5881 tgactcggta tcatcggagc gatatcgcac gcgtagtttc aacacggcaa atgcgatgac
     5941 aagtatgacg gccgcaacac cggcgatacc tcccacggcg gcaccggtat tgccgataac
     6001 gccaggattg cccggcatct ctcctgggtt gtgttcgtgt tcaggcgggg tgggcggagt
     6061 actggtgctg aatttcggct ctaccgtgat gttgagatcg gcggccgtat ctccgctgcc
     6121 gggaatcgag atgaggaacg gatcacagtc gtatgccttg ttggctttcg ccgaatccgt
     6181 gcgggagacc agatataggc caagcggtag gttctggaaa gtaagttggc cagctgcatt
     6241 ggtggtgccg gaatgctggt atagcttgtt ttttgccgcg t
//
LOCUS       DAWBMW010000089         6062 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_2923_length_6062_cov_3.322291, whole genome shotgun sequence.
ACCESSION   DAWBMW010000089 DAWBMW010000000
VERSION     DAWBMW010000089.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 6062)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6062)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6062
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2923_length_6062_cov_3.322291"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1800..1809
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1204 a         1819 c         1772 g         1257 t
ORIGIN      
        1 gggggggggg agcaatcgag tacgctcgtg agctcccctc agtcatctac gacgacagcc
       61 cctctcaaag aggggagcca aaaccactta gtacatgagg gtggctaggc gacgacgggc
      121 gcgggtcaga cgcgggtcgg tgggttcggg gatggcgaag tattcgagca gacgcttgcg
      181 aacctgctcg atatcggcct tgtgcccggc ggccaaaaag tccagcaaac ggtcgaaagc
      241 gtcttgaatc tggccaccaa tcatgtcgat atcagccacg gccaactgcg cttccacatc
      301 gtccggcgct gcggcggcag cggcacgtac gtcacgtaca tcagcggccc cggagcgggc
      361 cagcaacagc gccttcgcac gttcgcgggc agccagtgca tcggacgggt cggatccgag
      421 cacgcgctcg tattcggcgg cggcaccggc gtaatcgccc tcctcggcca aacgatgggc
      481 ttcggcatgc tccggcggca cctgctcagc acccgcgacg gcaccggtgg caccagcgct
      541 cttggcggca tcggaatcag gatcacccga gtacggcgcg gtaccgttca caccagactg
      601 cgcggcggcc tcaataatct tcggaatcac ctcgtcagta agttgcttga gctcgtcgtc
      661 acccggaagg ccctgcaaca gcggcatcgg acggccggca atcaacgcga acagtgcggg
      721 cacgccctgc acctgcagcg cttgggcaat cgagggattg gacgagatat cgatacggga
      781 gagctgaatc tggccgttca gggcgttcac cgcgtcgccc agtgcacggg ccatcgggaa
      841 caggcggtca tcggtcggca cccacagaag cagcaggatc gggaaggtcg cagaagtctg
      901 caccattgcc tggaaggtgt tttcggtggt gtcgatcacg tacccaccag cggcaggcgc
      961 gccgcccgct tgtccgggct cggccttgac ctgatgcttc agcgcctcca aatcgacggc
     1021 gcccgccaat gagactcccg gattgaactg ctgctctgcc ataatgcctc ccgattccac
     1081 gcgattcgcg tacaacaaca atcacatttg ctgacattga ttgtaatgcc gattcccacc
     1141 acgtagggat tcttccacta cgcggtggaa ttcccaccac gtagcaaaac gcccaccacg
     1201 atgtgacccc aaacggcacc attatgcgaa aggtaaagtt ttatcgacat cgtggtggac
     1261 gaatcacatc gtggtggggg cgttaaccgc gcggtgggtg gcggcaacaa ttacagcgct
     1321 tcgaccttta ccggctgacg gtcggcgccc actgcggtga tttgctcacc ggaacttgcc
     1381 aacggaatgt acaaggccac cacgttcaca tacgtgacct tcatggtgct ggtgtacttg
     1441 tcgctattga acagcgcctt ctcgtcgtcg gaagccggcc gcgattcgcg gccatcccct
     1501 gccttgcgag tccattcgcc gttaatacgg ccaaccacca gatcaccggc accgtctgca
     1561 gcacgcatca cccacatctg ttcaggcaca gcggtgaatt tttgctcctg cgtaccgttg
     1621 ttgcgttgca taccttcctg caccttggcc gtggtattgg ccagttcctg gcgcagcaaa
     1681 tcgtctgcga acttggaagc gtattggctc ttggagccgt tctgcagcac atcggcgtat
     1741 tgggccaagg catcggcggg agcggccacc agcttctcat cctttgcggt gcccatccgn
     1801 nnnnnnnnnc gagccaatag tcggaatctc gaacttcggc agcttggcgc cctcaaacag
     1861 tcgagccata gccatcagct tgtaattctg ctgggcggat tcctgatcga agaccagcaa
     1921 acgcttggac tgctgatctt cggtggtggt cgtgatggtg aacaccgagc gaggccatcc
     1981 gtcgtccgta ggaatcaccg tctgggcgat gtccttggga atggtcgcat attcggacat
     2041 accgccgcca cgctgattga tcgtggtctg gctgatgcga atgtccagct ggggcccgcc
     2101 cataacggtc gcatacccat cgggattctt ggcctgatcg gcttcatcga tcgtcttgag
     2161 aatcttcaga cgaatcttct tctcctgcgc ctcggtcaaa tccggcgcaa ccttggtgga
     2221 ggtatcagca gccggcgtcg gcagttggcc ttcacaggcg gtcaacggca cgaccaacgc
     2281 catgcaggcg agggcggaca cacacagttt gttccatcgc ttgttcatca gctttcctcc
     2341 tgtccgtcat cggccgagtc gtctccggta ttgacctctt gggccaagcg ggagaagtag
     2401 gcctgcagtt cgtcaggggt gattaccgaa gtcgattcgt tatcgccggc gctgaggccg
     2461 ggctcagcgc cgctttcggc accggtctgc tggtcggcga ccaggttacg cgaacttggg
     2521 tcgatgataa ccggtgtttc ggtggcttca gattgcgagg attgcgagga cgcacggccc
     2581 gaacgccgat gtgtggcgtg acggcgacga ttacgtcgac ttgcaccatc cggcttcccg
     2641 gacactgcga tgctggcggg tgcctcttcc tcgattacgg cgcttgccat gccttccacc
     2701 atgcgcttgc ggcgcttatg cggcggcatg gcgaacacgg aggcgcacag tacggccagc
     2761 acggccagca atccgccgga cagatagaac ggcatcgcaa agtccggcac ttcggagcgc
     2821 acccagtgca gctgcacctt ggcgttggtg acattttcgc ccaaatccac aatggctacc
     2881 gtcgaggcgc cggtcttctt gctcttcagc gaaacggacc cagtgccgca tttcacggca
     2941 gtccacatat cggaatcctt gaacgacacc gcgtcatcgg cactggtagg cgctccctga
     3001 ctcgtgctgc ttcccttctg ggtgctcagc tcgctccatg atgccaatcc ggtgatgcgg
     3061 gtataggact tctcggacgc gacccatccg gcaacatcct tggacgaacc caacgccacg
     3121 cacgcctcgt cctgagagga tcccgagtcc acggttatcg tggcattctg atccaacaag
     3181 gtcagcacat tcggatcagt gacggcatac cgggagccgc tcatggttgc ggaagcggtg
     3241 atttcagtgg agggcttcca catcgtggca ttcatgtacc ccaacaccac agccgccacg
     3301 gccaatagtc cgaagatagg cgtcaccact ccacgcatga ttttggcatg acgggatgga
     3361 cgcttggcgc ccttatccgg tcttgtagtc tgttcttcag cactcataac tcaaccattc
     3421 tacaatcttc acctgtttat tacctaggag cgcagaaaat caaggtttac cagtagggtg
     3481 atgtgtcatg cgtaagtctg ttaagtctat gttatctccc ctcggcaagg ccgttgccct
     3541 tgcctgctcg ctggcgatgt gcgtgtcgct ggctgcctgc tccagttcga gctcggactc
     3601 gaaatcctct tcttccgatt cctcttcatc ttccgactct tctgacaaga agggccaaat
     3661 tgccggcgtg accgccaagg gcaagctcgg cgagaagccg accatctcct ttaatacgcc
     3721 gatgaccgtg tccgacggct cttatgtggt gctgcagaag ggcgatggcg acgtgatcga
     3781 ggagggcgac cgcgtctgcg cccaaggcat cgcgctcaac gttaaagacg gaaccgagct
     3841 catggacacc tggaccaaga acacgccgga ttgctcgctg aaggtggatt ccaagaccct
     3901 ctcctcgacc tactacaacc agatcaaggg tgccaagatc aacaccacga ttggctttgg
     3961 cgtcaacgct caggattcgt ccgggtactc ttacattctg gcgatgacgt ttgtgtccaa
     4021 gtccaaggac ttggagaagg ccactggcga ggaagtcaag gatgtgccgg ccaatctgcc
     4081 gaaggtgacg cgagccaaaa acggcgagcc gtccatcgac atgaacggtc agggttcggt
     4141 tgattcgctg atctcgcaga cgctgatcaa gggtaacggc gccaagctga ccgacaagaa
     4201 caccgtggtg gtcaagtaca ccggttggct tacgaatggc aagcagttcg attcgtcctg
     4261 ggatcgcgat tccacaatcg acgccgatct gttcagcgat tccagcggcc agcatcaggt
     4321 tatcgagggc tggcagaagg gtctggtcgg ccagaccgtc ggctctcagg ttctgctggt
     4381 gattccgccg gatcaggcct atggagataa ggagcaaggt ccgattcccg ccaactccac
     4441 gttggtcttc gtaatcgata ttctcgccgc gtactaaaag atatagcagg ggctccctct
     4501 gatgagggag cccctgctat atctagaaca gcctgagact gtcgtcctcg gtgcctttca
     4561 tatcgtcgta atcgaggatg aggcaggtga agccgcggtc tttggccaag acctgggctt
     4621 gcggcgtgat ggtttgtgcg gcgaagatgc cgtgcaccgg ggccagcagc ggatctcgat
     4681 tgagcagttc gcagtagcga gtcagctgct cgacgccatc aataccgccg tgacgtttga
     4741 tttccacagc cacatgctcg ccctcggcat tcacggccat aatatccact gggccaatgg
     4801 cggtcgggta ttcgcgacgc accagcttgg ctcccttgcc gatacgctcg atttgctcgg
     4861 ccagatagcg ctgcagatgg tcttccacgc catccttgat gagacccgga tcttcgccca
     4921 gatcataggt ttggtcggaa tagatatgct gcagcgtcac ttcaagaatg tccgaaccct
     4981 tgtcggcgct gacacgtagc accttttccg gctcaccagc ggcgacactt tccggggaat
     5041 gcgtcgcaga atcaccatcc acagccccac cagcgacact ttcccgggaa tgttgcgcgg
     5101 atttggcggc tgggtcgatc tcacgaatcg tgcagggcgc ggtcatccag ttcagcggct
     5161 tgtaggagca caattcggag aaaatcaaca ggctggaatc ggccttgatc agcaatacgc
     5221 gcttggccag gggcagtgag gcgttgaggc ggcccgaata ctcggccgaa cagtcggcaa
     5281 caataactcg cataggccac cactgtagcg caacacatgg catacatagc aaaaggggct
     5341 ctctggaaaa ccagagagcc ccttaaggag ttggttcagg tcactcggcg gtgccggagt
     5401 actggacgtc gtggccgcgc tcgaccgcca cggtgagttt gttcgagtcg aaggaaatga
     5461 atccgtcggt cacctcgaac atgtggcgat cgccatcggg ctcaaccacg gtgagcgtac
     5521 cctgcttgat caccgtgagc acaggctcat ggtcgggcag gatgcccatg ccgccctcag
     5581 aggcgggaat ggtcacgctc gtcgcctcgc cgtgccacac agggtggtca gcggcgacga
     5641 tgttgacctt catcgtggtt ttttccgcag ccatcacgca cccagttcct tctgcatgtt
     5701 gtgccacttc tcttcgaggt cgtcgatgcc gccgatgccg gagaacgcct gctccgggac
     5761 gtcgtcgtac atgccgtcgc agatgcgggt gaaggcttcg atggtctcgt cggccggcac
     5821 gtaggagccg gggcgaccgg tgaacttctc agccacatag aagttctggc cgaggaactg
     5881 ctcgatctta cgagcacggt tcacggtggt cttgtcctct tcgccgagct cgtcgatacc
     5941 gatcagggcg atgatgtcct gcagctcctt gttacgctgc agaatcgcct tgacgcggtt
     6001 ggcgcagtcg tagtgagcct ggcccacgta acgcggatca aggattcgcg aggtggagct
     6061 ca
//
LOCUS       DAWBMW010000090         6054 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_2928_length_6054_cov_2.771962, whole genome shotgun sequence.
ACCESSION   DAWBMW010000090 DAWBMW010000000
VERSION     DAWBMW010000090.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 6054)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6054)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6054
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2928_length_6054_cov_2.771962"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1186 a         1845 c         1886 g         1137 t
ORIGIN      
        1 gtgcatgatg ttgcgcttgg attcctcacg gctcaaaccc agttcctcga ggatcttcat
       61 atcggccttg gtgctgacca gccgcaggtt ctcttcgggg gtgaggtagt cgatcaggtt
      121 atggtcctgg aacaccaggg acacgtgctc ccgacggtgc ttcgcatagc ccgatgcggc
      181 gatgtccttc ccctcgaagc ggaccactcc ccgggagggg gcgtccaggc cggccagcag
      241 agacagcagc gtgctcttgc cggcgccgga cgggccggtg atcgcataca tcttcccgga
      301 ttcgaaatcc gcgctgacat ccgagagaac aaggatcttc gccgcgccgt aggcgtaatc
      361 gacatggtcc agttgcagac agttagacat gattggtgac tcctaacgca tagaggaaag
      421 aatggatttc ggggatttgg acagcacggc ggcgcatgac acggcaaggg ccacggcgat
      481 gaccgcgcat ccgagcagca ggatgagcac cgtctgctca ggcggcaagg cctccacgtg
      541 caacgattcc aatggcgcga cgcccgaatc gcgcaacagt cgggaagaca cgtatcccga
      601 cagcatggcg ccggtgccca gcgaacacag ggcggcgacc gccgccatga gtccggtttc
      661 gatggcgagt tgtgcgacta tccgcatctt gccgatgccg agggccagaa gcgtgcctat
      721 ctcatggatg cgtgagcgaa tccagaagac cagcgccatc gccagcacca gtacgtccgc
      781 gaggcagacg gacagcagca ccatgcgcac cagatcacgc accgtctgca cggattgcag
      841 gacgccggag agccgggccg aatcgtcggt gacgtcgtac ttgccaccgg ccatggtctt
      901 ggcctgactg accgccgcgg acaggtcctg cggattatcc gacaggcatc ggatcatgcc
      961 gatccggtcg tttccggtca gagcggaggt cactcgcctg cccgaataga tgaggttctc
     1021 ggaagtgtcc gagggcaggg ggctttgggc ctgcacgttg ccggagaaga tcccggccac
     1081 acgcaccgtg gcgttgcgcc cctcctgccg gagacggaat gtcgagccga ccgataggcc
     1141 gttctgcagc gcgaagtcgc ggtggaccag cacatcgtcg ccgtcgcccg agatatggcg
     1201 gccctgctcc aggcggtaca ggccgctctg gaattcctcg gacaatgagg aatcggtggt
     1261 gcccagcacg ctgacctgca tggccagatc ggagtccaat cgggggccct gccgaggcat
     1321 gaccgggcgc gcgccatcca cctgcgccaa ggtcttcgtc gcataggcgg tcttcgtcac
     1381 accggggata cgcgagaacc ggctcgcctc ctcggcgctg atgggattct cgccggcgct
     1441 gacggtaaac cctatgccca tgcccgcgcc gatgccgtcg gacatggtac gcagggccgt
     1501 cgagactccg acctgcgcca ccaggctcgt gaagaccatc agcatcagca gaccgatgat
     1561 gccgctgcgt accggcttgc gtacgatcgc caacgtggca cgcttccaca cattcatgct
     1621 catcatcgaa tctccgacag gtctcgcggc gatttgcgca gcatcggcat gcacgccacc
     1681 gcgacgcaca ccagcatcac cgccaacagg ccgagcgcca cgtacagcat ggcggtcccg
     1741 tcgaccgaca ccgtgagcga atccagggtg cgcaccgaca tgctggattc aaggtcgccg
     1801 ccgacctgcc ccatgctgct cagctccttc gccgcggagg cgttcacgga atggagcgcc
     1861 gtggcgccga gccattgggc cattccctgc gcgcacagcc agccgagggc gagggacggc
     1921 agaccgatca gcaccatctc ggtgaggtat tgggcgacca gggagggctt gccgacgcca
     1981 agggacacca gtatgcccat ctcctgacgg cggtcgttca tccacagcag cagcatgaga
     2041 ctgagcacga gcaccgccga gacggtcacg ccgatcaggg cgccgcgcat catggagcgc
     2101 acgccgcggg cggcatgcag catgctcgac gaatactggt cgttgcgtgt gatctgatag
     2161 ttgttccaat ccaccgggag tttcttcgcg gcatccatgg tcttctccac gtcaacgccc
     2221 ttggacagca cgaaggtggc gtcctggtat atttccttgc cgtccttgta ctggtacagg
     2281 tcgcgtgtgg tatccagatc ggtgtagatg gtgttggcgg tgagttccgc tcggctggat
     2341 acctttctgg cgctgtcgcc cttgaatatg cccacgatgg tggtcttgac ggtcgccgtc
     2401 gaatgtgatt cgttgtcggc gtcgtaggcg ttggccttga gcgtgagcgt gtctccgact
     2461 ttgagcccgt tggctttggc gagatcctca tggatcatcg acgtgtactt gtcggaggcc
     2521 ttcagatgcc gtccttcggc catgccgagc gtgcgcgacg tgaacacgtt gagtttcgac
     2581 gaatcattcg tgcctatgac gttggcggcg ttgccgaatt gctgctcctt ggtcgcatcg
     2641 taatcatcgc cgccggggac cttcgccaca ttcgccccca cgagatcggc ggtggcgttc
     2701 tgacggacca tatacgaatc gaccccgggc agttgggcga tcctgcgcac gtcggcggga
     2761 cgcaccatgc cgcctccgcg aggcgtgagc atgctgccct gcatgttgct ggacagcaca
     2821 aatccaccga ccgcttcctt ctcgattcgt ccggcggcct gttgagccgc ccgagaaacc
     2881 gcgatcgtgg atagcatgct cgccgttatg atggtcagta ttatgaggag cactatggtt
     2941 cgcactcgtt ttctgagcac atagcgcacc gaccgtgcaa cgaacgtcat caatacctcc
     3001 tgtgggactt gggcttttcg cccaaaatgc gaaacatgcc gcgtttcgcc gattagttag
     3061 ccgcgacaat acatgacgca cctcaagata cgggaagaga tacgccttgg gcgaaaaacg
     3121 gaacagcggc aaatgtcgtt tattctggat tttggtccat ctcgggccgc gtcgatgcgt
     3181 cggcccgaga ggaatcagcg ctcgcgcctc accttcgagg cggcgaacac gaggagcggc
     3241 agagcggcga acgcatagtt ctcgcattgg atgtacaggt atgcgaccag gacgagccag
     3301 cagatgacgg tcttgtgcca gacgccggat tcgcccgaac ccgcgccggc aacgccatca
     3361 ccactattgc ccccggcaac atcagcaacg tcggcaacgt cggtgacaca aacaccgtgc
     3421 gccagcccgt cggacgatgc gccatccgca ccgccacggc ataccgtcct cccgcgcgtc
     3481 atgcagacca tcgaggctcc gaccagcatg gccagcccga tcagcccgta ttcggcgatg
     3541 acgctggtat acgcgcacat cgtccacacc gtgtccgcgt tcacgccggc cgcgaatccc
     3601 gtggccgcgc cgccgttcat gccaagaccg tccagcagcc gtccggcttt ggccgcgccg
     3661 gcccacaccg cattgatgat gttgcccgcg ccataatcgg tgaacagcgt caacggatgg
     3721 gtgagcaatc cgcatatcgg atcgaggctt tggtagatcc gcgcgaagaa cgatccgtct
     3781 ccctcggccc cgttttccgc gatggcggag agtctggaat ccgccagcac acccagcagg
     3841 cccagtgcgc atgcgccgag aatctgcagc atgccccgga cgcggcgtgc gccgtcatgc
     3901 caatcggtgc gcgccacaag ggcgatcagc aacgccacga cggaatcgat gacgattctg
     3961 gtgccggctt gcatgagcac cgcgccgacg gcatacgtga cgatgaggtc acgcagccgt
     4021 ttggcgtaga tgcggtcgcg cccgcgcatg agccacatga gcggaagcag tatgccgaac
     4081 aggtgcatgc cgatatagct gggctcggcg aacaggaact gcggacggcc gccgccccac
     4141 accgagttgt cgctgatgta ttgccggtac atgaggtgcg agaaatagtc ggtcaacggt
     4201 ttggcgtgca gacggatgga cagccattgc acgacgccca ctccgaagga aacccagtat
     4261 gatgcgatga gaatccgcaa tggcgtacgc cacggaatgc gtttgatgtc gaacgcaagg
     4321 gcgatcgcac ccaaagtggc gagcacgccc agcaatccgg tgatgctcat gaacgcggcg
     4381 ttgaggtgga tgccgaactt cagccatccc gggatggaca ggattatgca ggccaccggt
     4441 aggaggaaca ttggcaggta ccggttggct gcgatgcgca gttgccgcca attgcatagg
     4501 cagtacacgg cgaacagcgc cggactgatt ggagaccaga acggcgcgta caggccaagc
     4561 gtggtgccgt ccaccggaag aaaagcaatg gccagcaaca gccaaatgac gttgcgttcc
     4621 tgcgatgtca gaaatcgcag gaacgaccgg tattgcggtc ggccatgatg gttgcccgcg
     4681 tcgatggtgt cgttgcgtac gcatgcctgg ttcatgtgat tctccctccc cgtcaacctt
     4741 actcgttcat catgatgcgc ggacggggca tgctttcccc gctgaaagtg caatggcccg
     4801 aacattgaca aggtcgcacc gaatgccccc catcattggc acattgtgcg atctcgtcgc
     4861 ggctggctga cgaaataatg ttggccgacc ggttacattg gttcggtaca gtggcggcat
     4921 tattggccac tgaatcaagc gatttgcggc agaacgacga aaacaacact tgggcgaaaa
     4981 gaggcaggca tgactacgaa tcgaccgctc attgcggtga ctccgctgat ggattacggg
     5041 cgcgatagtc tgtggatgct gcccggatat atggaggcga taatgcgggc cggcggtacg
     5101 cccgtcatgt tgccgctgac cgatgacacc gacattcttg cgcaatgcgc cgaacggttt
     5161 gacgcgtttc tgtttacggg cgggccggat gtgggaccga tggttggtgc agcggcctcc
     5221 gccaccggcc gtagcgaggt gctctctcct gagcgcgacc gaatggaatc gattctgctg
     5281 ccagctgtaa tggcttggga caagccgatt ctgggcatct gccgtggcat tcagttcatc
     5341 aacgcagcgc tgcgcggcac gttgtggcag gatctgccaa gccagcatcc gagcgatatc
     5401 gaacaccata tgaatccgcc gtacgatgcg ttcggacata acgtttcctt ggtgcccggc
     5461 actcccctgg cttcattgtt cgcagggcag actgagattg cggtgaatag ctatcaccat
     5521 caggcggttc gtgagcctgc tgctggactc gaggtcatgg ccgtcgcgcc cgacggcgtg
     5581 atcgaggccc tgtaccggcc ggcaagccac ttcctgtggg ccgtgcagtg gcatcccgag
     5641 tttctctaca aggtagatcc acgctcgcag gcgatttttg atgcgtttgt ggggagttgc
     5701 cgataacagc aaaaggggct gctccggttt cccggaacag ccccttttca gcggtagcag
     5761 tcagcagact gatttagtca tcggtgctgg cttgccagcg ctgattggcg cgtgcggaga
     5821 ccgccgtccg aaggcgaagg cgtacgaagg tacgtcgaac ctgaggaaag gcggtatacg
     5881 ctccgccaat cagtgctggt tgttgagacg gtggtacatc tcggacagct ccagttcctt
     5941 ctcgaactgg cgagcggtgg tgttctcgtc gatgacggcg agctcaacac cggcgatgcg
     6001 ggcgaagtct tcccaagcct cacgaccaac ggaggtggtc atgcaggtgt ggtg
//
LOCUS       DAWBMW010000091         5997 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_2971_length_5997_cov_2.323628, whole genome shotgun sequence.
ACCESSION   DAWBMW010000091 DAWBMW010000000
VERSION     DAWBMW010000091.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 5997)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5997)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5997
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2971_length_5997_cov_2.323628"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3192..3201
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1222 a         1769 c         1736 g         1260 t
ORIGIN      
        1 cgccaacgag acgaccgcac cgtcggcagg catcggctcg tcgccggcga gcgcgcgcgg
       61 caattcggcg tccagttccg gctcctccat gagattcacc tcctgcacgc tggccttagc
      121 atcggcgaga gcggcgtcaa ggcgtagtgc ggccgcggca ttgccgccgg cggcctcgta
      181 caggcgcaca atcagatcgt cggaaccgtc atcggcgagc ttgacccagt ccaatacgat
      241 ggtgccggtg acgtcggtga gcgagaccag cggggccaag tcaggcaact cccctattac
      301 agggctgttg agtcgcgccg cctcgtccag cactgcgttc atatcggcgt cagcgaccag
      361 agaccatgcg aaggagtgcg cgccttggtc ggtgcgtggg tcagggtaca gcggtgcgga
      421 cagcagcgac aaccgcacca tggtgccgcg gccggtgcca tgtgccgtat cggcgtggat
      481 ggggctgacg tcggagccgt aggtggaggc gttgaccaca gcggcggcat agtcggcgtc
      541 ggcgatacgc acgaagcgat gcgtgcagct ttcgaatttg gcatcatcgc tgcgcgtgtt
      601 cttattgatc gggcgttcga ccaggccgta ttggcattca tattgcgcgt tcaccgcttg
      661 cacggtgacc ggcatatcca ctttgaggaa ctgttcgacg gcatgccagt ccacatcggc
      721 ggtgaagtcc agtgtggcgg agcccggcct taaggcaatg cccgtacgaa tttcaacgcc
      781 cttcgcgcga gtgacagcat gcacgacggc cccaccgttg gcggtttcat ccacgcttgt
      841 aatcgatgcc tcgctgagcg cgttggcggt gaggaatgca tcgcgttgaa tatcccaggc
      901 gtcccagtgg aagggttcgt ctttgagcat ttcgtagcgg cccagtcggg tgccggctgg
      961 caccagttca cgattggttc gctgatcgac gattgacgaa acggtgccgt cggcttcaat
     1021 gcgcacgtgc agttggccgt tgtcgagcac aatcgcatcg ccgtcatggg taacggcgac
     1081 ccgagccatg gatgcggttc ccgcttcggc tcggctcgaa gccgggcgta ccacccacgc
     1141 ctcacaggct tggcgggaat atggggcgat tacggcgtcg gttatggttg cgtcatcggg
     1201 ttccacggct gcgatcgcgc gcccggcttc aagggcgatc tcgttcagcc gagcgacgtc
     1261 acgcgcgtat tcgacccgcg cctgacggtg tacccatgcg atggccgaac cgggcagaat
     1321 gtcgtggaac tggttcagga gcagcgtttt ccaaatacgg tccagctctt cgcgcggata
     1381 gacatagtcc gggttcttga tgcgggccgc ggcgcacagg tattcagcga cgcgcagcat
     1441 cgactcctcc tggcggcagc cacgcttcat gtcctgctgg gcggtgaggg tggcacggtg
     1501 cagttcaagg tacagttcgc ccttgaacac cggggtctct ccttgcgcat cgtcgacgat
     1561 atccttacgc acgcggtcga acagttggtc gggagtaccg aattcgatct tcggcacgcc
     1621 ggcgagatca tggtcgcggc ggatgcgcgc ggtcatctca cgggtgggac cgccaccgcc
     1681 atcgccgaag ccgtacagca gaatcgcgtt acgggagagg tccttgtcca ggacgttgcg
     1741 ctgcgaatac atcagctcat gcatgctcat ccacgagcag taggtatccg agggcgggaa
     1801 gtgcgtgaga atacgcgtgc catcgatgcc ctcccacatg aacgagtggt gcgggaactt
     1861 ggtggtgtcg ttccacgaga tcttctgcgt gaggaaccat tcgtacccgg cacggcgggc
     1921 gatctgcggc catgcgccgg tgtagccgaa gctgtccggc agccatacgc ccttgggctc
     1981 cacaccgaga tgttccttga agtatttgcg gccatatgca atctggcgga tcagtgactc
     2041 accggcgggc aacatgccat cggcttccac ccacatgccg ccgaccggga tgaaccggcc
     2101 ttcttcaatg cgccgcttca tgcgcttgaa gatgtccgga tgatcctctt ccagccaggc
     2161 gtactgctgg gcggagctca tcgcgtattt gaagtcgggg tcggcgtcca tcagcgccaa
     2221 tgcgttggat acggtgcggg ccaccttgcg gcgggtctcg cgcaccggcc acagccatgc
     2281 ggagtcgata tgtgcgtggc cgatggcgct gacgttcatg gccgaggcgt tggctggctt
     2341 ggccagtacg ccggcgagtg ctgcgcgcgc ggcttccacg gattcggggt tacgctcgtc
     2401 gtaggcgttc agcgaacgct gcaatgcttt ggcgagttgc cagtagcgcg gcgactgttt
     2461 gtcggcgagt tccatcaggg agctcaccac gtcgagatcg acggagtagt tctcgtagca
     2521 ttcatcgaat tcggccagat cggcggactt gaatacgtac ggctcgtccg gcttgccggt
     2581 ggcatggtcg cccagttcag tctcaatgaa cggaggcacg ccgagcagca gcgggttgct
     2641 ggcggcttcg aggtacaggg tgaagctgcc gtcttcggcg accggcactt gcgcgttgcc
     2701 ctcagcatcc atgaacggca cccaatagtt gagcgggtgt acggctttga tggcggtgcc
     2761 gtcggcacgg tagacgaggc cctcgatatg gccgccgcag gagtgcgggt accagccgag
     2821 gtcgaggatg agctccagcg gcttgccttt cgggtagccg gccggcacgg taccggtcag
     2881 gtggaaccag actgtgcccc atgtggtgcc ccattcaccg ccgagcgtaa acggcttgaa
     2941 gtcgatgtca cctcgattga gcttctcgaa gaactcgtca gtgggcatcg gctctccggg
     3001 aatgtcaaag gactccaccg caagctgtgc gatcgatggg tggatgtgag ggtccacgcg
     3061 ctggcgcact atgcgcttgc accgctccag ttgctgttca ggtttcagga acatctgctg
     3121 ttcactcccc ttatctctgc cgcttgtgcg gccacatcat tgtgctgccc gttcatggga
     3181 aacatatcga cnnnnnnnnn natcgacatg cgccctcgcc cgcagaagac taaagcgctt
     3241 tagtaacata tttgagggaa cgggaaatga tgtcaaatta ctatcgcaat tcagcgtgtt
     3301 ccgtcaggca tgtcatgctc ggtgtcgatg acgatggtca ccggcccgtc attgaccagt
     3361 ccgacccgca tatgcgcacc gaaacgacct tccttcaccg gcactccccc ggagcgcaac
     3421 gcctcgtcga acttgatcca catgaaatcg gcatgctcgg gcttgccggc cttgatgaag
     3481 ctcgggcgat tgcccttgtg cacgtcggcg aacagcgtga actgcgagat ggagagaatc
     3541 tcgccgccga tgtcctgaat cgaacggttc atcttgccct gcgcatcctc gaacaccctg
     3601 agattcagaa tcttatgggc cagccaggcg atctgcttgt cgccgtcctc atcggtgacg
     3661 cccaccagaa tcatgaatcc cgggccaatc tgctgcggct cgaacgtggg atccagcgtg
     3721 cccaattcat tgaccacgtc cacggacgct tcggatactc gttgcaatac gactttcatg
     3781 gttccctaca gtacaacgtg tgcagtcacc agccggtaac gtacgcgacc gactggcgac
     3841 cggcaatatc tgcactgcag ttcatcgttt accgcttcat tcgacgacga gaccgatagt
     3901 catcacgaac accaccaaga aagtccggcg cgttgaggtc ctcaaggtca gcacctccgc
     3961 gcacggcggt cgcggcacag gcaacggcct caaacgaggc tttgattccc tgtccatcgg
     4021 catgaaaatc agtggcgcat cgcggtgaga taccaaaatc accagccgtc tgttcggcgt
     4081 cgatgttcgc gcccagaaac acaaactccc aatgatagcg ttcacgctca taggtaatca
     4141 tctgcttgat ctgatgcaac gaatatcgac gactcgcgtt ctcatacccg tcagtaatga
     4201 tgacaaacag cacatgttga gcacggtacg ctgccgcagt atgtcgttgc gtcacgatca
     4261 tgcggtcaat ggccagccca atcgcatcca acaacgcagt gctaccgcgc acccagtaat
     4321 cccggctcgt caaatcgttc acttcgcgga cgtcgaggtg attatgcaag gtttcgattc
     4381 gatgatcgaa cagtagggtc gttaccctgc actcgccggc aattgttcgc tgcttggaca
     4441 gcatgctgtt gaagccaccg atggtgtctg attccagacc tcccatcgat ccgcttctgt
     4501 cgacgacgaa caccagttcc gtacggccgt tattgccatt gtcgttcttg cccatgatga
     4561 atcctttcgc tcattcgcca gccggattgt cggcgatatc aatgagcttc gcatcatggg
     4621 accagtccta ggtcgcttgg caagcgacaa tatgccgcgt atcggcagca ggtcaggaga
     4681 cgagcgtacc gctccgcatc attgcccaag caacggctga tcgtatcgga acaacatctc
     4741 gttaatagtg aatacatcaa acatctcatg gacgatgaag aattccacga tcacatcgaa
     4801 tttcacgctg tgcgacagcg catacccggc gcacgccaat atatcttgcg tctcgtcaat
     4861 actcagccgt aaggcaatgg ccagggccag aaccgttttc ttgctgggca tatagccatc
     4921 gccacagcga attttagaga acagtttacg attgatattc gctcgtttat atacctccac
     4981 gtcggaatat cctttggcgt cgataagcct caagagaatg atgttaaaag gttcatccag
     5041 attggagagc accgcatcat ccacatgccc ggcggatttc ggcgatggag ctgccgacgg
     5101 cgccgagtag acaggcgttt cacccatatc gcccaggaaa tccggcatgt ccaagacgcc
     5161 ggaaggtacg gccctatccg ccttcggctt attatccgaa aaccgttgga gagcaagcag
     5221 ctcatacgtt ctgcttccta gcgtatcttc atgctccgct acataataat catcaatata
     5281 tgcgcgaaga cgtttgtcaa tactgctgct tatttcgaaa gcggttttgt cgaatacgca
     5341 taggtatacg tcgagatcgt gtccggagtc ggctgcgtcg cccaggaatc ggcgaatctc
     5401 atcgagggcc acatgaaggg cctcggcttt cggatatcca tagataccgc tggaaatcaa
     5461 ggggaaggcg atgctggcac acccctgccg tgccgcgagt tccatcgaat tgcgatagca
     5521 cgaacgcagc agacgttctt catcatgctt gccgtctcgc catatcgggc ctgcggtatg
     5581 aatcacatag cgtgatggca aattgaatcc cggagtgata accgcctcac cggtatgaat
     5641 gggagcaaac cgatcgcagg cagcagccat gcgcgagact cccgcagcgc ggaatatcgc
     5701 gccgcacact cccacccatg gcgagctgcg tgtttgccgc attgactatg gcgtccacct
     5761 tcatcttcgt aatatcctgt cggaccaatt ccagcggcat atatcctccc tgattacggc
     5821 gtagcataat agcctctaca catcgtgcaa tcggcagccc tgaactaagt ctaaatcgtt
     5881 tcttccccgc aagttcgtcc aactgacata tccgacacga gagaatagcg ggaaaacaag
     5941 gaatcaacgg agcaataaca gaaagccgct cagccttact ttcgtaaagc caagcgg
//
LOCUS       DAWBMW010000092         5960 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_2989_length_5960_cov_2.334970, whole genome shotgun sequence.
ACCESSION   DAWBMW010000092 DAWBMW010000000
VERSION     DAWBMW010000092.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 5960)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5960)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5960
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2989_length_5960_cov_2.334970"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4622..4631
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1101 a         1734 c         1865 g         1250 t
ORIGIN      
        1 tcagatcgga gagcttgtcg gcaattgcgt ccacattgcc gagcgtggcg ctcatcagca
       61 ggaactgggt ctgcggcagc gtgagcagtg gcacctgcca cgcccaaccg cgctccggat
      121 caccgtaata atggaattcg tccatcgcca cgaggcccac atcggcatgg gtgccttcgc
      181 gcaaagcctg attggcgagg atttcggcgg tgcagcagat gatcggcgcg tccgcgttga
      241 tgtggctgtc accggtgatc atgccgacat tgtcgcggcc gaacacttcg accaagtcga
      301 agaatttctc cgacaccagc gccttgatcg gagcggtgta ataggagcgg cggccagtgc
      361 atagggcggc gaagtgcatg ccgagcgcca ccagcgattt gcccgaaccg gtcggggtat
      421 tcaaaatcac atggtcgccg gccaagaggt ccataatggc ctcttcctga tgcggccacg
      481 gctcgatgcc cttgacgtct tccacccagc cgaagaaccg ttcgtagatg tcgtcggccg
      541 taagattccg ttcgcgtccg tcgccgtccc attcgggggc cagccgcccc agggatccgt
      601 attccttctc gtcagtcata atgctcagtc tacctagtgg cctattccga cggcacgagc
      661 cgccctccgt cgctatgcga cgcctcccgc cagcgggcgg agccatgcga agagctgtgc
      721 catgcgcttg gctccgccaa tgggaggatc gctcacgcca accggcggcg cacctcgtcg
      781 gggtccacct cgggttcgag gggaggccag gtcatgttca tgttcttcaa ggtggtgcgc
      841 agcagttcgg agaccacggc tcgcgaatac cagcggttat cggccggaac gaggtaccac
      901 ggtgcggcgg ccgtgctggt tttctcgaac acttcctgcc atgcggccat atactcatcc
      961 catcggtcgc gggcctcgag gtcggagata tcgaatttcc agaacttggt gggatcgtcc
     1021 agtctcccaa gaaagtgttt tttctgttct tcctggctta ccacgaggaa gatcttgagg
     1081 atggcgcatc cgctggcggc cagttcacgt tcgaaatcgt tgatctggtc gtagcgggca
     1141 cgccatacgt cttcgggata ggtgccatag acgtgcggca tgaccacatc ttcataatgg
     1201 gaacggtcga atacggcaat ccaccccgcc ttgggcactt cacgcttcac gcgccagagg
     1261 aagtcgtggt cgagctcctc ggccgcaggc ttgccgaagc catggtaatg gatgcccatc
     1321 gggtcacctt ggctgaacac gtgacgcacg atgccgccct tgccggaggc atccatgcct
     1381 tgcagcacga tgagcagccg ccgtgtcgag cctttgacgc cattcgcgta catgagccgc
     1441 tggtatcggg cgatttcgga ggagctgatc gaaatgaact cctcggcgtc ctctttattg
     1501 ccgtcgaatc cgggagtgga atcgccttcg acatcagtga cctgcatgcc gtggtggaac
     1561 ataagacgtt gagcgggcgg caacgaccat acggcgctga gcagttcact ggatttcgcc
     1621 gcacgtgcca gtcgttcagg cacgctggca ctggagtcga tggtgtctat gcgatccgcc
     1681 gcgagctgta ataccttgtc gatccgtcga tcggttttgt attcctttcc cttgccgttc
     1741 ttgttgttct ttcctgagga ttttccggct gtaccccact tgccggcctc ttcaggcttg
     1801 ttggttctgt cgtcgtcctt ggccatgctc ccgattgtac gtcttgtaac tcaggagacc
     1861 cagcaacgta gcgacattca cattccactt gttactgcaa atctatagac atgcagtcgt
     1921 tcggaccgta attgttggct ttgcaccaat tccaagcctc atccgtcgag tcgaagccct
     1981 caccggcaag aatcacatag tatttgtcgg aattgctgct gtaggtgggc cagtcacccg
     2041 accagataag cagtacgttt gacttcttgt tgtgcatcgt gatgaattgc gcaagaatat
     2101 cgtgatagtt ccaggtcttg ccttcagctt ccatgccctt gcgcttgctg gacagctgcg
     2161 cggtatacgt ggaatagacc cgaaaaatcg aacacggcat cggccacgac attatcgtcg
     2221 ctgtcctgca acgtgagtcg ggtggccgaa tcattgaatt tcgtggatgc gttcttatca
     2281 tcacaattcg aagtggccag aatcgtgact atcagattgt cgctctcgct cttgatcgag
     2341 gagactttca tatcggcagc ggcatcgcag gttttggcag gctttgcctt gtcggggtcg
     2401 atgtatgcca ccagatatcc gccggctttg atgacggtgt ccgtattcgt tacttcgctg
     2461 gtgccgatgg tgtagtgctt cttggctttc ttgtcactgc cgtagatgcc cagaccttca
     2521 cgtcaacgtc tgtggagccg gtgttcttga gctcgaccca atcgcaacgc ttgctatccg
     2581 ggtctccggc agctttcgta ttgccggcat agacctcgtt gatgattacg gatggtttgg
     2641 cagccgtgtt ctgttcggca gtggtctgct gctcgtccgc agtcgcagcg ggcacgccga
     2701 tcagggcgat gctgctcagt gtcaccgcag cggtgagaag cgcgactgaa ctttggctga
     2761 attactgctg aagctggttc ttggatttgc gtcgaaccgt ccgtattccg ccgataatcg
     2821 cgattacagc ggtaccgata gctacgctga tcaacgccgg tcgttgcgcg gcatggaggg
     2881 tggcgagatc cggtccggcc gcaatggcac tgttttcacc cgccgagcta tccgacgccg
     2941 ggtgattgcc cgcgtcggtg tactgggcat tgaccacact cgggccggta agcgccactt
     3001 ggtcggcggt ctcgaccttg gccacatcgc cgtgcttatc caaggtgacg cgggcgcaca
     3061 gggctttggc ccagttgata cgctgccagc cgtcgctcga ttgccatagg gggtatccgg
     3121 aatccaaggc agtggctttc aaaatcgcgt tgcgctgatc ggcgtgcagt tcgggatagg
     3181 ccagtcgcag cacgtccgcc gccccgcgcg gtgccacgaa atcagcgccg gactgggcgg
     3241 tgtcttgcgg gaaggtgtag gtcaggcgtg cggtgtagac gcgcacggcc gaggcctggt
     3301 ccgcgaccgg ctcggtggcc accgggtcca gaaaatcgtt ggtgtagccg cccgaaccgg
     3361 aggccttggt gttggcgatg caggcggcga gggtagtgcc gtggccgtcc gcggcgcagc
     3421 gggacaccag atagtcccgc agctgccggg acgcgggcac gatggacgaa gcgaattcgt
     3481 tatgccagta ctgcccgttc tgggcggatg cgccaatgcg cccgcccatg atgtcaagcg
     3541 gataatgcac gccgagcacg atgcggttgt tgccggcctc cgaagtgcgg gccatgattt
     3601 gcggcgcgag ttcgggaatc atgccggcca gcgcgatacc ccacgaatag gcgccggtag
     3661 tgtggccgga ggggtaggaa ctgtttttct gcaagttcga gtagccgggc acgtgttcga
     3721 gccatgaggg tgattgcttg atgtcaagcg tggcgggcag tccggcgaga tcgttagtgc
     3781 cgccgtagct gctttccgca cggttcagat aggggcgcgg atgcatggcc gcacgttttg
     3841 aggcgtaggt ggaggccacg ttaaaggaga agatatcggc gacggcgttg agtttcccgg
     3901 atttgagccc ttcgctcata tatttgccaa gcaccggacc gagcgcgtcc ggcatggctt
     3961 cctcggcttg cagatcggag tcgacgagcg cacgcttgcg ttgggcgttc aggccatcct
     4021 tatcgagatt ttcggcggct ttgtggttaa tggccacggc caaatcttcg tcgagtttca
     4081 ttgtatcggc gtcgagcacc ttgccaccgt gttcggcatc ggtgggggcc tgccaatagt
     4141 tcttttcgta gtcggagagc aatgccaccc aatccgggtt gggcagctgg tcggcctgtg
     4201 cgattgcagg cgtcacggct agcgtggccg ccaacgccag cggcgcaatg gtcgcaatag
     4261 caaaaacgga ggataggtgc cggaacatct tcatatgttc caacctatcc tccgtgaagg
     4321 ctacgtagcc ggagcccagt caaacactca gtaaattctt cgtgccggct tagcggcgat
     4381 tgagctgact gtcaggcctc tggcttgtcc tcaggctttt gagactcctg gaattcgccg
     4441 gtttccgggt caacctgtga ctcgggctgg gtggcgtcat atccctgttc gtagcgggtg
     4501 cgccgccagt tgcgaatcga ggcgttggtg ccacggtgat gcacatggta tttgcgcggc
     4561 gtaccgccca gcggcagctc ttcgacctcc ttggccacag caatctggtt gacattggac
     4621 gnnnnnnnnn ngacgggtcg ccggcggcga tcggcgccag tggctcgggc tcggcgggtg
     4681 cagccgtacg ctgctgcatg cgcgcgggca gccattcggc gagcctcgac agcagccagc
     4741 aggcgatgaa gtaaatcacc gcggccatga ccagcgtctg caggatgttg aagtacatcg
     4801 aaccgagtcg gcgcgattcc tgcagcaggt cggtatacat gatgatcgag cctagggcgg
     4861 tgtccttcaa caccacgacc agctgggtga cggcggccgg cagcatggcg tagacggcct
     4921 gcggcacctc gatctccatc agcgactgcg tttcggtcag gcccaaagcg agcgaggcct
     4981 cacgctggcc gcccggcaga ttgccgacgc cggagcgcac cagttcggcg accaccgaac
     5041 cgttgtacag caccaaggcc agcaccacgg cccaatagga cgggctgggc aggcccgcga
     5101 acgcgaacca tcgccagaag aagatcatga gcagcagcac cggcaccgca cggcagaact
     5161 cgacgatcac ggcggaaacg ccacgtacca gcgggttcgg cagcagtcgg cccacgccga
     5221 acaccaggcc gaacaccacg gcacccacca cggccacgac ggtggccttg atggtcatcc
     5281 acagtccggg caggtagaaa tcggtccacg cttcacgttc gatggcgggc ttccacagct
     5341 cccagctgag ctgattctcg ccgtccggcg ggttgtgcag gcgcatgagg ataagcacga
     5401 gcaccaccgc gaacagaatg cctgcgatcc agttgacgat gcgaatggtg cgccggccct
     5461 tgggaccggg cgcgtcgaac agcacggaat tcgaagtatc ggccatatcc gtcacctcct
     5521 tacggcgagc ttgttggaca ggtacgtggt gagcatgccg atggggatga tcaggatcac
     5581 gtagccgagc gcgaagatca ggaagatctg gatgatcacg tcggggcgga attcgatcat
     5641 ctcgctcatc agcgaggatg tttcggtggc cacggatgcg gcggcggcca cggtggagtt
     5701 cttgagcagt gcgatcagcg tgttgccgag cggcgccacg gagcctcgga aggcctgcgg
     5761 caggataatc tcagtagcgg actgcatgaa gcccaagccc agcgctcgtg cggcctcggc
     5821 ctggccaatc ggcacggtgt tgatgcccga acgcagcgat tcgcacacga acgccgccgt
     5881 gtacaggctc aggccggtga ccgcgagcca gaagaagttc gtggcgaacg tgtcggagaa
     5941 gctgagcttc agctgcgcat
//
LOCUS       DAWBMW010000093         5809 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_3083_length_5809_cov_2.943170, whole genome shotgun sequence.
ACCESSION   DAWBMW010000093 DAWBMW010000000
VERSION     DAWBMW010000093.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5809)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3083_length_5809_cov_2.943170"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    417..426
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1089 a         1715 c         1693 g         1302 t
ORIGIN      
        1 gcttcttgat ggcttcggcg ttcttctcgt cggagccgtc gatggcgccc ttggccttga
       61 gctcttcgag cagccaggtg cgggcttcgc gagcgttggt cagggtctcc agggtggtga
      121 aggtcttgga gacgatgatg aacagggtgg tctccgggtc gaggcccttg gtcttctcgg
      181 ccaggtcgtt cgggtcgatg ttggagatgt agcgggcgga gatgccagcg tcagcgtacg
      241 gcttcagagc ttcgtacacc atgacggggc ccaggtcgga gccgccgatg ccgatgttga
      301 ccacggtctc gatcttgcgg ccggtcacac cggtccactt gccggaacgc acgtcgtcag
      361 cgaaagcgta gatcttgtcg agtgtctcgc gcacgtcctt gacggtgtcc tgaccgnnnn
      421 nnnnnngtgc agcacggcgc ggtcttcggt gttgttgatg tgcacgccgg tgtacatagc
      481 cttggtgcgc tcatcgagct tgacggcctt ggcaaggttg gcgaacagct ggagggtttc
      541 cggcttgatc aggttcttgg acagatcgaa gtgcaggtcg ccggcgtcga aggacagctt
      601 ctcgacgcgc tcggcgtcct cggcgaacca cttcttgagg ctcacgcctt cgacctgaag
      661 ctcgtcatag tgcttctgca gggcagccca ctcaggggtc tgggtggcgt caacgggcgg
      721 attgatggcc ataggattcc attcctttca tcagtgactg gagcgccctt gcacacggct
      781 cggatgcgct ccgtatcacc aaacagaata ctcacaaatt cggacatgaa cagaacaaaa
      841 tgagtcagcg atgtcggatt gccatcgttt gcattatcga acagttcacg gcgcggcgca
      901 accgtcatct tcgactgagc ggtcgcgaca caaccgccaa tccgccgatt ggacttttcg
      961 tggggatgtc ttatacttaa cttcttgtgc gcggctggcg aaatgccttc cgcgaaccaa
     1021 gccactttag ctcagtcggt agagcggctc actcgtaatg agcaggtcgt cagttcgatt
     1081 ctgacaagtg gctcggatgc cctaggctgg ctggtctgta atggctgtcc ggcctagggt
     1141 tttgcatatc ttccacacct gtggaaacgc ggatatgcac gcgcgagtgg cggaattggt
     1201 agacgcgcag gatttaggtt cctgtgtctt tgacgtgtgg gttcaagtcc catctcgcgc
     1261 accggcttga gcctcttgga agcattggat tgccaacgct tccgggaggc ttttcgtata
     1321 tgcgcgcggc agctgcaagc ccttgcggac acctctacat atggcaatgc caaggcggca
     1381 aatataatgg gccacagcat tgcgggtctg agcctgtgcg ccatgagcct gtgtgagtca
     1441 aggagagaag tgtatgtccg ccattcttac gccggttggt ctgttgctga tcatcctcgc
     1501 cggctatctg ttcaagcggt tcggcctgtt cgggcagaag gattatcgcg tgctgcaaac
     1561 cgcggaattc aacatcgtgc tgcccggtgc catcgtatac tcgttcgcca ccaatccgca
     1621 tgacatctcg ctgctgctta tttcggtatt cgcattcctg tttgctttta tcccggtcgt
     1681 gttcatcttt ctggccaccc gcaagcgcaa tgtgaccgac cgcgccttcc tgatgctcaa
     1741 tggcgccggc ttcaatctcg gttgtttttc tttcccggtc gtccagtcat tctggggtgc
     1801 gggcgcggtg gtgccggctg cgatgttcga tatcggcaac tgcgtgatgg tggccgccgg
     1861 caccaatgtg ctcacccagc aactgctgca tattcagccc ggcaaaacac ttgccgaaca
     1921 acacgccggt tcggttccca ccttgccgta cgaaaagccc aaggatcgcg acgccaaacg
     1981 gcttgcccgc cgcgcattgc tgcgcaccat cggcaagagc ttcttcggct cggtgccgtt
     2041 cgacacgtat ctgctcatga tcgtgttgac cgtcgccaac gtgaagattc ccgattggat
     2101 cgcctctata acccagccat tgagcggcgc caacgcgctg gtgtcgatgc tgatggtggg
     2161 catgctcatg gatttgcccc aatccaagca tgacgtgaag gaagtcatgg ccgtcatcgc
     2221 ttggcgtata ccgttcagcg tcgccttcgc gttgatcgcg tggctcctgc tgccgttctc
     2281 ggcttcgatt cgcgccgtga tggtgatctg cgcgttggct ccgatcgcca ttttctccac
     2341 gctgttcacc gacaaagtac tcggcaacgc caaactggcc ggcttctcgc tggcaatcac
     2401 cgcgatgatt tcactggtga tgatggctgt ggctcacgcg ctgatgggcg tgtgagcaaa
     2461 tgcgcaagta ggcacacggc gggtgcacgg tcagacgaga cgcagcccac ataggctcgt
     2521 cccccgactg ccagttgcta ccggcgcagc agcttgtgcg ccagattgct cacgccgaag
     2581 cggaacttat ccaccggcaa cgtgaatttt cctaccatct gctcgacgta gccgttaaag
     2641 ccctgcttga attccagcac gccgcggccc tcgtcgttcg gatcgtcgaa cacgccggaa
     2701 atgccgtaga agttgtagcg gctcacgccc ttctccacac atagatgcag cattgcttcg
     2761 tactgaatca gcgccgaggc gtagaacggc ttgtactcct ccaacgaacc ggaatactga
     2821 tagaccacct cattggggtg actgacgaac attgaagccg ctgccggcag cacatcgccc
     2881 ttcctggcat atccctccac ctcagccaga cgcttgtcgg cggccgcgag attgcgtgat
     2941 tcctccccca gctgacgttc gatacgcgtg gtcggacgtt catcgtattt cgcctgcaat
     3001 cgagctacct tggcctgcaa cgcctcgcgc ttggcggtca tatccgccac atattcggcg
     3061 atgtggattt ccgcgagcat gaaatgcgca tctgcgccat acgcctgctt gaactggcgg
     3121 aaatagtctg cgccacgcgt cgcgaaccca cggcgttcac cggtccgccg ttcgatatcg
     3181 gcgaacacgt cgaactcgtc ggggccgatt tcgcgaacgt gcacacccat cgactgcgcc
     3241 cgcttgacac tccactgagc acgtttgccg aatgacgcca gcagttcctt gccgttggtg
     3301 atgccggtga gatccttgac gtacatccac tgacagacca cgtcataacc ggtatggaat
     3361 ccgccatgcg tccagccggc atcggcgaat ccggccagta gtgcatcgtt cggctcgccg
     3421 tcgggctgcc cgtccgagga atacagacgg taggcgacgt taggccagca tgctacggaa
     3481 acagcatggt ggcgacgggc tgattcgcaa atcgcctcgg ttatggcccg cagcagagcc
     3541 ggatcatcgc taatgccgat ggggccgaaa tatacggagc catctgcacc gaaacgacca
     3601 ttgaggtaca gaattacgca gccggttacc agattgccgg atgcgtcgca cacgccaacg
     3661 cattcggctt catgcccacg aatcttggca agacggacct gcccggacca ttgctggaag
     3721 ctgccctggc ttgtggcttc ggaaagccgg tcgagctctt cgggcgtaat cacgccaaac
     3781 gtatacgtcg tatctgttcg attcgcagtc gcgctcatct cgtcgtcacc tgtgccttat
     3841 ttctgtgtcc gtgctcgcca ctgtttgtca gcgatgcagc agcttgtgcg ctgcttcgga
     3901 tacaccgaag cgcagtccgt ccaccggcag cgtgaacttg cccggaagct cttccacgaa
     3961 tccgccgaag cgagtcttga actgccagac gccgtaggaa ggatcgttgg cgtcgaaaat
     4021 gccggaaatg ccgtagaagt tgaagcgctc cacgccacgg gcgatgcagt caagcatcgc
     4081 ctcgaagtgg attagggaag acgggtagta atccatgaaa cgatcgtcct cgccgctgga
     4141 caggcagacg acctcgttcg gatgccagaa gtagacgccg gcttccaccg gaatgaccgg
     4201 gccgtccgaa gcgatgatgc cattgacttc gccgatggtc ttttccagcg ccgcaatctt
     4261 cttgtctgcg gtggcgatgc gcttttccag cgttgccggg tcggaagcac ccttcttggc
     4321 cgtgccttcc ggctgctgtt gcgcgcgctg gagcaggcgt tcgaggttgc gcttgtcttt
     4381 ggaggccgca ttcagcttgg attgcagggt gtccagatat tcgtcgaaat gggtttcgac
     4441 caccttgaac tcgatcaggt cgccgaaggt atcgaacaga cgctcatagt aggccagatc
     4501 gcggctcttg aagccttgac gtgcggcgct catatcgcac agcttgacga aagtgctcag
     4561 ttcgccgcgt tccagtcggc gcacgcgcag accggaatcc tgggcaatgc ggatgttctt
     4621 acggcggtac ttggagaacg aggccagcag ttccttctca ttatgaatgc cgtcaagccc
     4681 cttgacgaag ttccagcggt tcaccacgga ttcgtagccc acggtgaaac cgccatgttt
     4741 ccaaccgaag cgggccatgt tgtccatcat cgtggtgtcc gggtcgctgg tcggcttgcc
     4801 gtccagatca tgcagacggt agactgcggc cggccagcag gatacgctga tggcgtgatg
     4861 ggccttggcc gaacgacgca gggcttcggt catcgcctca accacctgtg gattgtcata
     4921 atcgcacagc ggtccgcacc agaccgagcc ttccaaaccg aaacgggacc gcgtgtacac
     4981 cacgtagcag gcggcaatca gcttgtcacc gcgcttgaca cccacatagt ccaccggacc
     5041 gttgaacgca cccatggcgg ccatctgcga ggtctgctgg aaactacctt gcacttgagc
     5101 tgcggagaac gcatccagct cggcaggagt gatcttggta ggggtgagcg aggcgacgcc
     5161 tgcatcggat tgcgtcataa tcgaaccttc ttacttgtcg ttactctgat cgttactctg
     5221 ggctctggaa tggcctgtgt gctgcgtccg tttaccgcga cagcagcttg tgcgcaaatt
     5281 gcttcatggc ataggcgacc ggtttgaccg gcagtacgaa ctcgcgtaca ccacgcccct
     5341 cgtcatcggg atcatcaaag acgccgtcga tgccgtagaa gttgtagcga gacagcccgt
     5401 gttccacgca gaaatgcagc atcgcgtcat gctgaatcag cgcggaggcg tagaagggct
     5461 tgtactgctc gacgctaccg gagaacaggt agatgacctc gcgcggatgc tccacgaata
     5521 gtgatgccgc agccggcagt acatccccgt ctttggcgaa ctcggccgcc tcgttcaggc
     5581 gcttttcggc tgccgccaga ttgcgggtct catcccccag ctgacgttcg gttttggtgg
     5641 tggggtgttc ggcgtttttg gcggtcaatg ccgccacctt ggccgaaagc gcttcacgct
     5701 tggacgtcat gtctgctacg tattcgtcga tgtggatctc ggcaaccata aaatgcgctt
     5761 tgctgccgaa cgcttccttg aaacggtgga aatatgcttc gccacggta
//
LOCUS       DAWBMW010000094         5770 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_3111_length_5770_cov_2.565354, whole genome shotgun sequence.
ACCESSION   DAWBMW010000094 DAWBMW010000000
VERSION     DAWBMW010000094.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 5770)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5770)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5770
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3111_length_5770_cov_2.565354"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4201..4210
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1158 a         1735 c         1718 g         1149 t
ORIGIN      
        1 ggatggcacg ttgacgccgg tcgtggcgca gggtgccggg gcttcgggcg tgtccaagtc
       61 cgcgctcgaa gcggtgaagg ccgccactac gatctcctcc gccgaagccg atgtggccgc
      121 agccgcagct gcggccaaga agaccggcga acagtccacg ctgaccggag ccttgaagat
      181 cgaggacgag ccgtgcgtgg tgatgatgag cgctaccggt ttgattgccc gtaccacgcc
      241 aagcgcgatg gacgtgttca atgcacgatc cacttccgat gagcgtttga gagacgatca
      301 gatcaccaca atcttcgaga cttccacgcg cgctacgtat gggctggtca cgtcggccgg
      361 ccgcctggtg ctcgcgcatg tggttgattt gcctgccttg cccgctaccg ccacattgtc
      421 gctgaaggga ggagttcagg ccgacgaact gatcggcatg accgaatcca ccgatccgat
      481 tcgcggtgag cgtgtgatta cggcgattgc catggagcag ccgacgtccg gcaagaccag
      541 tgccaaggac gagagcgagg atggcggggc cgccgaggcc aagccgttgc cgtcgctggc
      601 tatcggtacc cgtaacggtg tcatcaagcg ttggaatcgt gaggcaccca ccaccatgga
      661 ttcttggccc gtcattgatc tgaaagatgg cgacgaagtg gtattcgccg ccgtggccga
      721 ggatgacgac cgtctggtgt tcatctcctc cgattcctcg ctactgacct ttgaggcgaa
      781 gaacgtgcgc ccgcagggtc gtactgcggg cggtatggcc ggcatcaagc tggccgaggg
      841 cgcccgtgtg gcggccttca acgtggttcc cgccggcaag gtggcatgga cctatgaaga
      901 gggcgagaac ggcttgactt ccggatccgg tgcagtggtg ctcaccgtgg ccggcgattc
      961 cgatgcactg cccggcaccg aaaacggcgc ggcgaaggtc actccgctgg aaatgtaccc
     1021 caccaagggt cgcgctaccg gcggcgtgcg ttcccagcga ttcctgaagg gccagaacac
     1081 gctgattttt gcctgggtgg gcctctaccc gttgcacgcc tctacatccg caggctctcc
     1141 ggtcgagctt cccaagcccg atatgcgccg agacggttcc ggagtagacc tcgcctcccc
     1201 catcgccttc attgcgtaac ctcaattaag ctccctctga tgggccgagc cgtaaagcaa
     1261 acaccccagt ggggtgtttg taggcgagga gcgaacgata gtgagccaaa caatagagaa
     1321 gagaccctaa acccggccta cactaagcat taaggaggtg gcgatgagac tactggttgc
     1381 cgtatccaat gaaccgttgg cccacgccat tcatggtgcc ctgcttgccg gtcgtcatga
     1441 ggtgtctttg gcaagcaccg gtagtatcgc gctcgccgct gtcgccaagg agtcatttga
     1501 cgcagtgatt cttgatgaat ccctgctcgg catttccacc gagcagctca tcgaatccct
     1561 acgcaatgcc cgtgcctccg tcaagattct gctgctgact tcgacgccac tcagcaaaac
     1621 catgggtgaa ggcagccgct ggcgagcgct gcctgacgag tacgccccta tcgaagagac
     1681 gccctacagc tccttcagcg cgaattcacg cgcaaacaac tcagatgccg aagccgaaca
     1741 acgctcggac aatgctgcct ctagcgcaca agctaacgta cgacacccgg atgtcgtgct
     1801 gagcaaacca ttctccgatg gcgaactgct ctcatacgtc ggctcgcttg ccaccgactc
     1861 ccccgccatg atcattcgtg gcaatctcac gttggacacc acgaatcaca aggcctacta
     1921 cgcatccact cacagcgcaa tctcactgtc ccgtctggaa tacagtttgc ttgaagcgct
     1981 ggtcaaggct gatggccagt tcgtcaacac cagcgagttg gtcaaactta tcggcggccc
     2041 attcttcgag caacagggct tactcaagaa cgccctctac acactggata agaagctcac
     2101 ccgcgccggc ctcatcatga cccaacgagg ctccaactac cgcattcgat agctgggctc
     2161 aatcagtaca gccgcatgcc ttggggaacg ggggtgaagc cggactggtg gaggatgcgg
     2221 gcgaaggggt gacgggcgtt gaggggttcg ccattgatgt cgctgaatgt cacggagccg
     2281 gtgccattgc gtttgagggc gtaggccagt tctgtggcgg ccttgcgcat ttgttcgtcg
     2341 gaatcattga cagcggcttc atggccgacc gattgatcga acgctgtcag gtgcttggct
     2401 ttggcggccg cgtatagtag cgcgccttgc gcattgagca cgaccgtggc tccgatacgg
     2461 cgtgccggtt tacccgcacc ggcaggcgcg tccggccacg cgatggcgga gccactgaga
     2521 tttgcggggt cgagcacact caatgccacc acggactggc cacgatgctc tgacggatga
     2581 cgcaacgcat ccacggtgtc gcgctcggca aattgggccg cgccgaaacc acggacgaac
     2641 atgcctcgta ccagtcgacc atgctcctcc atgcgattca ataccggata caacgctgag
     2701 tagccacccg ggatattttc cttgtcaatc accggctgcg cgatgacgcc atacctgtcc
     2761 agcaacgtct cgaccagtgc gagtgcccgt tcggtctggt cggtgtcttc ggcttcagct
     2821 tgcccgatta cggcggacca caaacctcct aacgtcatat ccgtagtggc tcggcgaatc
     2881 ggagcgacac gcccacgacg acgtgatgta gtggatttac gcgggcttgc tgcgcctgcg
     2941 gttaatgcac gcaccggcgc gaagctggaa ttggtgacct taccttgcca gaccaagctc
     3001 cacaaggcat ccttgaactg ctgttcgctc cactcttgcg gaatgatttc gccggtgttt
     3061 ggattgatgt ccggctccgc ggtctcattc cacgcacgtt tggcggcatc catcagttga
     3121 cgggcatgga acgctccccc tgagtccagt gcggtcagaa tcgcttccgg catggtggcg
     3181 tctgaggcgt tcgatgtcgt accatgttcc acaccgctga acaatattga atccgccgga
     3241 tagaaagcaa cctcaccggg gtccatcgca ctggttcccc cggtttttga gccgacccag
     3301 accacatcgc cgctggtcag caactcatcc agcaatgcag gttggtagtc tcgtactcgt
     3361 gcggggaata ctgccgactc ccagagtgca gtgggcagtg atatgccctc cagctgctcg
     3421 ataacgcgca tgagcccgtc agcaccttca tatcgttcgc cgccgaccgg cccgatacct
     3481 tgtcggtcga taagaaacat ctggaacgcc tctgattcta ctggcttgac tgctttacgc
     3541 gccttggcca atgagcgaga ccgaatacgg cggaatacgt ccttatggag ccactgttgg
     3601 ccttgctcgt catcatctac aaagtggcct tgcagcaact cgcctttggc agccagcaca
     3661 tccagagtgt gcacggcatc cgtagcatcg aggtgtagat cggctatgag ctgatcggcg
     3721 gtgaaaggac catgggtttt ggcgtaacgg gtcacgcgac cagtcacatc atcgtcggca
     3781 agctcattcc agaaagtgtg ctcccccagt tcctgttcga cttcgcgaat cacgtccggg
     3841 ttgagcactt gtgccatatc ggtggtgccg agcaagcgtt cgagcacgtg cggatcgaga
     3901 gacagcagtt gcgcgctacg ctcggcctga ggttggtcat actggtacat caccacgccg
     3961 acgaagccaa agagaatgtt ctcggcgaaa ggcgatggcg actcggtttc ctcgtccttc
     4021 acggtaatgg tgccggcttg caatccggtc atcacctcgt tcagggcggg catgtcgtat
     4081 acgtcttgca aacattcgcg cgcggtttcc agcagcagcg gaaagttctt ggccatacgt
     4141 gcggattgca ggagctgtgc ggcgcgcagt cgctgctgcc ataggggcac gcgacggccc
     4201 nnnnnnnnnn gcccgggtcg gatcgcggca tgaacagtga tcgggcggcg cattcgcgga
     4261 atcgtgcggc aaatagcacg gactcgccca cttgacgctc tacatctgct ttgaggtctt
     4321 ctgggtcgaa aaggaacagg tcttgagcag gaatatgtcc gtcaccgtcc ggtagctgaa
     4381 tgacgatacc gtcatcggcg gcatatacct gcccgtcgaa accgtagcgt tggcgcagtc
     4441 tgcggctgat ggccatggac cacggttcgt gaacgcggcg gccgaagggg cttaacagca
     4501 cgatgcgcca tccgccgtct tcatcgcgtg tgcgctccac gataagggtt tggtcgctgg
     4561 ggactgcacc agtggcggct tgttgctcgg agagtaggcg ggccaagttg gtgatggcgt
     4621 tggcatctag gccatcgtgg tggagacggg tgaggattgt tgggatgaag gttgggacag
     4681 ctggaccttc tgcggcagac ctcccctcag tccgcttcac gtctaatccg gcggcgattt
     4741 ctcgggtgaa gcgggctatg gtacggctga aaccgtagtc acggccagcg ccttcaccgt
     4801 gccagaacgg caggcgggcg gtgcggcccg gagccggagt cacgaccaca cgatcacggg
     4861 tgatttcctg aatctgccat gtggaggtgc cgagcgtaat gacatcgccc acgcgcgatt
     4921 cgtagaccat ctcctcatcc aactcgccga cgcgacgctg gcctttgccg gcgtccgcct
     4981 caggcaggac cacggtgtac agccctcgat cgggaatcgt accgccagag gtaacggcca
     5041 ctttctgcga accgggacgg gcggaaatca ggccattatc ccgattccat accagtcgcg
     5101 gccggaaggc cgagaattcc tcgctgttgt attcgccgga catcatgccg attaccgcat
     5161 cgaacatgtc acgtggcagt tcggcaaacg gcgcagcacg acgcactgtg gcataccagt
     5221 catcagcgtt caaatcgtgc atcgcggcag cggccacggt ctgctgggca agaatatcga
     5281 gcggattgcg cggcacggcc aatggttcta tatcgccggc aatcatggct tccagacttg
     5341 cggccccagt gacaatctgc tgacgggtga gcgggtagaa caatgcgtga gagacgccac
     5401 cgacctgatg atcggcacgg cctacacgct gcaatccgga ggagaccgac aacggcgtat
     5461 cgacttgaat caccaagtcg accgagccca tatcaatgcc gagctccaaa ctggaggtgg
     5521 ccaccacgca acgcaattca ccacgtttaa gccgttcctc aatcattttg cgacggtctt
     5581 tggaaaccga cccatgatga gccatggcga tgacatcatc gccctcatgt gagccgacaa
     5641 gcatggtggt ggagccgacc acagcatcgt aatgcgtgga gaacccttcg cggccttccg
     5701 gagagcccag atcccgcaca gtgtcgggat tggtgccatg ccgggtctgc gcatacagat
     5761 cgttcaagcg
//
LOCUS       DAWBMW010000095         5740 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_3131_length_5740_cov_2.629024, whole genome shotgun sequence.
ACCESSION   DAWBMW010000095 DAWBMW010000000
VERSION     DAWBMW010000095.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 5740)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5740)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5740
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3131_length_5740_cov_2.629024"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1181 a         1692 c         1683 g         1184 t
ORIGIN      
        1 atgaattgtt gcgtatacaa caatgtgggg tgaaggtaca aacgcacacg gcccccgcat
       61 ggtacggagg acaatggatc agcatattct cagcatcgaa atgcgcgcgg taaccaaagc
      121 cgtcgaccgc tatttgggcg agagcatgcc gcaaaccgcc agggaaacca ccggcggcaa
      181 cgcgcatatc atcatgttct tggcgcgtaa ccgcggccgt gagatatacc agcacaccat
      241 cgaacagaaa ttctgcatca cccggtccac cgcctcgcgt gtgctggccc tgatggagaa
      301 gaagggcctg atcgcccgtg aatcggtgga gcatgacgcg cgctgcaagc gcatcgtcct
      361 gaccgacaag gcggacgcga tcgtcgccga tctcaaggcg aacggcgatc gggtcgaacg
      421 gttgctggtc ggcggcttct ccgatggaga gaaggccgct ctgcgcgact acgtcgcacg
      481 catgcgtgcg aacatcgagc gcgcacaacg cgaatttgaa catcaaacac tccctcagtc
      541 acctgtggtg atggctcccg atcaagacgg agcagaggtt gctaacacga aggaggagaa
      601 cgaatgagcg ataccgcaga ggcaatcgcg caggcgcgca aagccgtcga accggacaag
      661 cccaaacatg tactgcgcac gttgggccag tcgattcgtg aatacaagaa agccagcatc
      721 ctggctccgg tgttcgtgac ggtcgaaggc atcctggaga tcctgatccc gaccatcatg
      781 gcttcgctga tcgatgaggg tatcaccggt ggcagcatgc cggccaccgt caagttcggc
      841 gtgattctgc tggtgtgctc gatggtttcg ctgggtgccg gcttcctgtc cggaaagtac
      901 gcggccgtgg ccggcgcggg cttcgccaag aacctgcgca aggaccagtt cgagaaggtg
      961 cagggcttca gcttcaccaa tattgaccgg ttctccaccg gttcgatcgt gacccgactg
     1021 accaccgacg tgacgaacct gcagaacgcc tacatgatga tcattcgtct gggcgtgcgt
     1081 gccccgatta tggtggtcgt atcctggctg ttctcgttcc gtatctcccc gtcgatttcc
     1141 atggtgttcc tggcctgcat cccgattctg gccatcggcc tgtgcggtct ggccgggctc
     1201 gtgcatccgg tgttcgagcg cgtgtttcac acctacgatg cgttgaacaa cgtggtcgac
     1261 gagaacctgc agggcatccg cgtggtcaag tcctataacc gcgaatcctt cgaggtctcc
     1321 aagttcggcc gcatctccca gcgcatcttc aaggacttca ccaaggctga gcgcatcatg
     1381 agcttcaaca gcccgctgat gatgatctgc atctacggtt cgatgatcct catcgcatgg
     1441 atgggcgcgc agcagatcgt cgcctccggc aacaaccccg ccgtgggcct gaccaccggc
     1501 gacctgaccg cactggtcac ctacgccatg cagatcctta tggccatgat gatgctgtcc
     1561 atgatcttcg tcatggtgat tatttctcag gcttctgccg agcgtatctg ccaggtgctg
     1621 caggaggaaa gcacggtgca gaacccggca cagccggtga ctgacatggc cgacggctcc
     1681 atcgaattcg accatgtcac cttccgctac tccgacagtt ccgaaaagcc cgtgcttgac
     1741 gacatcaacc tgaagatccg ctccggcatg accgtcggca ttgtcggcgg tactggttcg
     1801 gcaaagtcgt ctctggtaca gctcgtgccg cgactgtacg acgtgagctc cggttcgctg
     1861 aaggtcggcg gcgtggatgt acgcgactac gatctcgagg cgctgcgcga tcaggttgcc
     1921 atggtgttgc agaagaacgt gctgttctcc ggcactattg ccgaaaacct caggtggggc
     1981 aacccgaacg ccaccgatga agagattcgc catgccgcgc agctcgcgca ggccgatgga
     2041 ttcgttcagg aattccccga taagtacgac acgtacatcg agcagggcgg taccaatgtg
     2101 tccggcggac agcgccagcg cctgtgcatc gcccgtgcgc tgctgaagaa gccgaagatt
     2161 ctgattctcg acgattcgac cagcgccgtc gataccaaga cggacaagct gatccgttcc
     2221 gcgttccaca acgagattcc ggacaccacg aagatcatca tcgcccagcg cgtggcctcc
     2281 gtgcaggaat ccgacatgat tctggtgatg gattccggcc gcatcatggc gtccggcaca
     2341 cacgacgaac tgctcgtcac ctgcgatgaa taccgttcca tctacgaatc ccagaccaag
     2401 aaccaggccc agcccgaaga actggctgct gccgaacagg ctgctgagtc atctaccgct
     2461 gagccgtccg aaaccgtgac ggtgacagtg cccatgccca cggccgatac cagcgcagct
     2521 gataccaatg cagccgatac caaggaagga gaagcacgat gagcaacgac cagagtttca
     2581 agaaccgcaa gcccgccatg ggcaaagccg agcctggcac catcaagcgc atcttcagct
     2641 acatcttcca gtacaagtgg cgcgtcatcg ccatcgtggt gtgcatcttg gtcggtgccg
     2701 ccgcgcaggc cggttccgcg ctgttcctcc agtcgttgat tgatacgtat attctgccga
     2761 tggtgaggga gtcgaacccc gactgggccc cgctgctgcg ggccatcagc ctgatggcgt
     2821 gcctgtatgt ggccggcatc gtggcgtcct ggctgtggca gtggctgatc gtcaccgtcg
     2881 agcagggcac gctcaagaag attcgtgacg acatgttcgc ccaccagcag aagctgccga
     2941 tccgctactt cgacagccac gagcacggcg acatcatgag ccactacacg aacgacaccg
     3001 atacgctgcg ccaggccatc tcgcagtcgt tcccgcagat gttctcctcg attatctccg
     3061 cggtcgcggc gctgctgtcc atgctgtggc tgtccattcc gttcaccgcg ttcgtaatcg
     3121 tgttcaccgt tctgctctac ttcatcgtgc gcaagatcgt gagccgctcc ggccgctact
     3181 ttgtcaagca gcagcagtgg atcggcgacg tgaacgcctt cgtggaggaa tccgtcaacg
     3241 gccagaaggt catcaaggtc ttcaaccatg aagacgccac ccagaatacc ttcgacgaga
     3301 agaacgagga actcttcgag gcgtccgccg aagcgaacac ctggggcaac gtcaccatgc
     3361 cggtcgtcgg caacatgggc tacctgctct acatcctgct tgccatcgtc ggcgccgccg
     3421 tctcgctggc cggcgtcaac gacatcggtc tgaccggcgt caagccgctc accctcggca
     3481 cgctcgtctc cctgctgacc ctatcccgct cgttcatcaa cccgatcggc caggtctccc
     3541 agcagctgac catggtgatg atggcgctcg ccggtgcctc ccgtatcttc aagctgatgg
     3601 acgaacccat cgaggaagac aagggcaccg tgacgctcgt gaacgtggaa cttggcgagg
     3661 atggccgcac catgaccgaa gtcgaccacg agaccggcca ctgggcatgg aagcgcgagg
     3721 tcggcgacga cggtacgagg tctctgaagg ccgcagaaaa gctcaagggc tcggcccgcg
     3781 aggtggcgat gaaggccaag gagcaggcga tcacctcgcc cgacggccga ctgaccctgc
     3841 tgcgcggcga cgtgcgtttc accaacgtga ctttcggcta caacccggac aagccggtgc
     3901 tgcacgacat cacctggttc gccaagcccg gccagaagat cgcgctcgtc ggcgccaccg
     3961 gtgccggcaa gaccaccgtg accaacctca tcaaccggtt ctacgacatc cagcacggcc
     4021 agatcctgta cgacggcatc tccgtggccg gcatcaagaa gcctgacctg cgcaggtctt
     4081 tgggcatcgt gctgcaggac gtgaacctgt tcaccggcac cgtgatggac aacatccgtt
     4141 acggccgcct gaacgccacc gatgaggaat gcatcgaggc cgcgcgtctg gtcaatgcgg
     4201 acagcttcat ccgtatgctg cccgagggat acaacacggt gctggaaggc gacggttccg
     4261 gcctgtccca gggccagcgc cagctgatct ccatcgctcg cgcggcggtg gccgatccgc
     4321 ccgcgctgat ccttgacgag gccacgtcct ccatcgatac ccgtaccgag gaggtcgtgc
     4381 aggccggcat ggataacctg atgaagggcc gtacggtctt cgtcatcgcc caccgcctgt
     4441 ccaccgtgcg caattccgat gtgatcatgg tgctcgacca cggcaacatc atcgagcgtg
     4501 gttcgcatga cgagctgatc gcgcaaaagg gcgagtacta ccagctgtac accggcgcgg
     4561 tggaactgga gtagccggta cgcctgcaaa aaccgcacct cgattatcgg gttgaagcaa
     4621 ttctcatccg gtggttgagg tgcggttttc tatgttgaga gccgtgtgtt agcgcaggat
     4681 tacgaatatt ggcggtgaaa agggtacatt tgaattgttg ttttgacgat gtatcggccg
     4741 caaggagggg actatggcgt tcgtgacgtt gaaggatgtc gctcaacgtg ccggtgtgtc
     4801 ggctgctgcc gtctcccaaa tacttcacaa taaaggccgc ttttctagcg atacgcgaca
     4861 gttggtgctg aatactgttg aggaaatggg gtatgtgccc gatcaacgtg ctcgctccat
     4921 gcgctcgtcg gatactaaga ctgttggttt gctggttcct gatttgcgca atccgtattt
     4981 cgccgatttg gtctcgtcaa tggaggatga gttgtatgcc cggggctatt ccacgctgat
     5041 tggtacttca gcagaaacag tggaacgcca ggatgcgttt attgacaatc tgctcgggca
     5101 gcgtattgat ggtgcgatcg tggtgccgca aggtgtcaat tcgcctggta tgcagtcgct
     5161 tatcgcgcgc gagctaccac tggtgttcgt ggatcgtttg gtatcgggag taaattcggt
     5221 gccattcgta gtgtccgacc catacccagg tgtttgcgag gcggttgccg aacttgtgcg
     5281 cttagggcat cgtcacattg gatttgtgtc acactcgtca ctgggctcgt ccaatataaa
     5341 tgagcgtgaa gcggcattcc gttctgctgt ggcacaggtg acgcagttgg gggagggaac
     5401 tgcggcggtt gtcgactgtg attctacata tgtttcgcgc gaagcaggac taaatgaact
     5461 tgtaagagca ggtgttacgg cgattatttg cgcttactcg cctgacatga tcacgatgat
     5521 tggtttgctg catgaccgcg gcattgatat tggtagtgaa atgtcagtga tttcattcga
     5581 tgacattgcc gtattccgtc tgctgactcc gcaagtcgcg attatttcgc agcaagctga
     5641 ggatatgggt cgccagggcg tggatatgtt gctcgacatg attataaata atgattgcag
     5701 tcgtggtgaa tcgcgctatg tcccgtcatc gtttgtactg
//
LOCUS       DAWBMW010000096         5664 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_3179_length_5664_cov_3.016046, whole genome shotgun sequence.
ACCESSION   DAWBMW010000096 DAWBMW010000000
VERSION     DAWBMW010000096.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 5664)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5664)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5664
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3179_length_5664_cov_3.016046"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1292 a         1865 c         1524 g          983 t
ORIGIN      
        1 ataccgcgct ttgggctgca atatattcac ggcgcaacgc gaaagccaaa cgccattgca
       61 ccgacaatcg cgcgtcctcc gtcacggcgt ccaagagacg tggctcttcg tgaacgccta
      121 caccgagctc atccaatcgg gccagcatat cgcgcaactg gctgataaac gcatctgaaa
      181 tacctcgcgt gaacctatcc gcgctggtcg agccgcctgc cgacgtcgga gacggcgaat
      241 ccgacccttg ttgcgagagg cttgattgtg cggaagcggc cgagccgggc atctcgccgg
      301 caccggcatc gcgtatgagt ctcgcccagt catcctgggc aaagtagacg cgcagcaaat
      361 cacaggtgcc gcaatcatct cccgctacgg catggcgcag atgcacggcc atgacttgac
      421 gcagcaaagc atcctgttcc gcaccgttca gcaatcgagg cgacggcaat ccagttccct
      481 cgcgcgaagc cgatatggcc cggaatgcca cggcacccag cgtggtgacc ggcctggcct
      541 gcacggaaaa cccgagctca cgaatcaccc gattgcccaa tgcgtcggcg gccacacggc
      601 cggatacggc catcaccgcc tgcgtgccac cgtagcggcg cagccccgcc atcaacaccg
      661 ataaggcgaa ttcagttttg ccgcttccag gcgtgccgac aatcagcctc gccgctccat
      721 cacggtcgat gccgtccagc actgttccag gttcaagtct cgtcatcatg gctccagcct
      781 aaacccgacc gtcacagagg gaaaaatttt accgaacccg acgaagaatc gtacccttga
      841 cggtatcctg tagaacgaat gcagggagag cgcattttcc aaggagggag ggaacagatg
      901 gacaacattc cgatgagacc gccgaagctg cccggatatg atttcgtcca gatgttggga
      961 agcggtgcca ccgccacggt gtacctatat aaccagcgta tgcccgcacg cccggtggcg
     1021 gtaaaggcca gcgccaaaac ccttgaccca cgggccgctg ccctgttcaa ccgcgaggcc
     1081 aacttcatgg ccaagctgtc atcccacccg tacattctgc cggtatatgg tgccggcgtc
     1141 acctccgatg gccatggcta catcgtcatc gaatatgcgc cgggcggcac ctacaacagc
     1201 atcatgaagg cccggtccat gacctgcgag caggtactcg acctcggcat caagctgtca
     1261 agcgcgctgt tcaccgcgca tcgcagcaat atcatccatc acgacatcaa gcccagcaac
     1321 attctcatca cctcgcaggg cttgcctgtt ctgggtgatt tcggtatctc caccgatgtg
     1381 tatgaccgca ccgaaaccgg attctcgccg ccgtgggcac cgcctgaggt gcttcagcat
     1441 ctgaccaatg gttcggaagc cgccgacatc tattcactgg cggccaccct gtacgccatg
     1501 ttcgtcggct gctcgccatt ccagcacgat tatcacccgc gttcgcaatc ggaactgatt
     1561 tcgctgatcg tcaaccagcc attgcctcgc ctcaaccgcc ccgacgtgcc ggccgatgtg
     1621 gaagcggtgc tgcgccgggc cctgaacaag gaccccgacc agcggtacta ctccgcgttg
     1681 gagttcgcac gtgccatgca gcgtgtgcaa ttcgcatatt tcgggcatgc cactcccgtt
     1741 accgtggaag ggatgccggc ctatcccaaa gacatcgagc gtcggcgccg ggccacggac
     1801 cagcgagatc agatcacccg ttcgtcacgg cgatgggtca aaccggtggg tattaccgtc
     1861 ggtatacttg cggcgatcac cgcagcggca ctgatattcg ccttcgtggt cgttccccgc
     1921 atggacagcg ccaccggatc agataccaaa caggtggcga cgcccggcac caaggatgat
     1981 gacaacaacc gcaataatca ggatgacgtg atcaccgata ccgcgaacgc ccagattcct
     2041 tccccggaga atctgaacgg cacctatgcg gcggatgggt catccgtgac gttcacctgg
     2101 acgaatccag acccgcaaag cggtgatacc tatgcgtggg ccatagtgca gggcagcaat
     2161 accgaccaga gcgcgcaaac cgtcacaacg tcagatccca cggtgaccgt caacgcgcag
     2221 gaaggctcgc agacctgcat tcaggtgtcc attgtgcgca agaaccgcca gatgtccgtc
     2281 agccccgcca tcgcctgtgc ggccaagccg caagaatgat ccgctcgcca ctgccgggca
     2341 tgccaccctg gcattgcgta tcgcatcaga cgtgcgccca gcagcggcga attcaccaaa
     2401 tggacaacag gaacggcagg tattggaacc gtaaagcgtt gtcattacac cagcaaccag
     2461 agaaaacagg ggtcagggag ttcagatggc cacatcgtcc cagtcatacg atcgtcgccg
     2521 acgcatcagc gcggctttgc gccgccttat gccctccgga gggcatacgt ggacggttcc
     2581 cgtaatcacg ctcctgctgc ttctgggact tgttgccggc gcggtgatca tgagcaccgt
     2641 cacgcgccgc catgtgcaac tggacgatgg caccgtatgg gtcacttcgc tgaaaaacca
     2701 gaaggccgca cgattcaacg taaagaacaa ggaggccgac gccggcgtct cctcctccgc
     2761 cccacgattc gacatcgcac agcataatgg cgacaccatc ctgaccgaaa cgaccaaagc
     2821 ctccaccatc aaggcatcca cggtcagcac cggtgtcaag accgacatca aagccaacac
     2881 caccactgtg gtcggcgggg ataccgcagc cctgatcaac gagaagaccg gcaatgtgcg
     2941 gaccggcttt tcggaaaacc ttgattccgt gacccccacc acttccgatc cgaaaataaa
     3001 gctgggcgag ggcgggcgca tcgcagtaac ccacgatggc aaggtctacg gataccgtcc
     3061 atccgacggt atggtgctgc gtcttgacaa tcccagcagc gccaaggcca aaaccctcga
     3121 atcgctgacc gacggcaagc agcagaccgt tgaaagcttt accgtcatcg ggagcacgcc
     3181 ggtaatcgca accggcaaaa ccgtcatatt caagggcggc cgcgtcgatg tcgacacgac
     3241 cggcaccctc acccttcagg agcctcccac cgatgacata cagtccgatt gggtggccgc
     3301 ggcatcaccg agaggccttg cgcttatccc gttgaagtcc aatgccaagg cgaatttcat
     3361 agccaacggg ggcaaggcga atccagcacg accggtatcg tccaagggct gcgtgtactc
     3421 ggcttggagc cagaaggcca gcaactacat tcgcgcctgc tcccccacgg ataccagcgt
     3481 caagccgcaa accctcgaat ccgtcaatac tacttccgaa ttggtgttcc gcaccaacca
     3541 tcgccttgtg gtgctcaacg acacggttaa cggcaatgtg tggaaccctg aggattccac
     3601 caaagtcatc aaaatacagt ggaacaaaat acagaccgag cagaccgaga aagaacagca
     3661 gaacaacgac tcggccaaca accaccacga cttctccaag acctgttccg cacaatccgg
     3721 ccaaatcaaa gccgaggatg atgaaatcgg cgcacgagcc ggttcggaac agattctcga
     3781 cgcgctgcgc aatgacgagc agaccgattg ttccgtactg aaaatcacca aggttggcgc
     3841 accaaacaac aaagacgtca ccatttcgcc gatttacgac ggccggtatc tgcaactcga
     3901 tgcctccgcc gcatccgcag gcaccgttac ctttacttac gatatttccg atggtcgcgg
     3961 ccagacctct tccgccacgg tgaccgtaac cctgaatgat ggaggcaacc acgctcccgt
     4021 acagttcgac actcctcccg agatcgatgt ggagcaggga gccagttata ccgccaatgc
     4081 gctgagcagc ttcaacgatc cggatggaga cccgctcact ctggtctccg ctgtggccca
     4141 gaacaccgac caggtgcagg tgtccacccg agccgacggc cagcttacgt tcaacaccgg
     4201 tgcgctggcg tcgggacgtg tgggcgtcga ggtcaccgtc tccgacggca cggcaaccgg
     4261 caccggcatg gtgtatttct cggtcaagcc cgcgaacacc ttggccgcgg tcatcgaccc
     4321 ggtagcgaaa accaccgttc ccaacacgga taccgtcgtc aagctgtcct cttatgtgca
     4381 tggcacttcc ttgcagccgg cacaactgac tcaggtggat acgcccaacg gcgcttcgac
     4441 gaccaccaat gcggcggata tgtcgttgac gttcaaggcc accaatcccg gcacctatta
     4501 tgtgccctac atcattactc aaggttcgat tccggccacc ggattggctc gagtcgaggt
     4561 gcagcccgcc accggcgagg ccgccaaacc ggttgccgcc aacgatgtcg ccctgcttgg
     4621 cgcggataat accgccattg tggagccgct caccaatgac gtcgatccga tgggcggtgt
     4681 gctgtccgtc accacggtga gcgcaccggc cgactccggc atcaaggttg gtctcgtcag
     4741 ccataagcgc gtgtacatca ccgcgcgtca ggtgcccacg aagcccgtcg cccttaccta
     4801 caccgtggcc aacgcctccg gaaccgccaa gggcaccatc gtgttgcagc cgccggctct
     4861 ggccacctcg aattcagtac ccaaggccag caatatcaat gcccaggtgc gtaccgacgg
     4921 catcgtgtcc gtggatgtgc ttgaccatgt ggcctactcg gatggcacca cggtcaaact
     4981 caagaacaac ctgcaatacg acaaggggac gttcaagggt ctggccttcg tctctggaga
     5041 cacggtccgc taccaagcat ccagcgagac cggcaacttc cccatcacct atacggttga
     5101 agacaatctg ggtaatgtgg cttccgccaa tatcaccatt accgtgcata agagcgacgc
     5161 agcgagcaag gccgcgccaa ccccgcatga cgccgaagcg caggttgccg ccgggcagaa
     5221 ggtccgcatc cccatcacac tgaccggcat agacacggat ggcgacgacg atcagctgtt
     5281 gggtctgggc aacaaggcac ccacgctggg ccgcatatcc gaagtcggtt ccgactactt
     5341 ggtctatgaa gcgtatccgg attcgtccgg caccgacacc ttctcctatg ccgtggagga
     5401 ttggaccggt cagcgcgcac aggcgcagat ccgcgtgggc gtattccaag gatcgtccga
     5461 ctccggcgta tacgccaggg atgacgaaat cacgttgagg cccaatacgg ccgcaaccgt
     5521 gccggtcgcg cagaacgaca tctccggcga caacaccaat ctgaccgtcg acaagcatgt
     5581 cgaggtccaa ggtctcgacg gggtgagcgt gaaagacaat atgctctcct tcaccacgcc
     5641 cgacagcgcc accaccgact atat
//
LOCUS       DAWBMW010000097         5583 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_3228_length_5583_cov_2.527135, whole genome shotgun sequence.
ACCESSION   DAWBMW010000097 DAWBMW010000000
VERSION     DAWBMW010000097.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 5583)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5583)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5583
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3228_length_5583_cov_2.527135"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1752..1761
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1135 a         1782 c         1580 g         1076 t
ORIGIN      
        1 accacaacga gatcacgttc tccatggtgt acgcctactc cgaacactac gtattgccca
       61 tcagccacga cgaagtcgtg tacggcaagg gttccctgta cggcaagatg ccgggcgatg
      121 actggcagaa gctggccggc gtgcgttcga tgttcgccta tcagtgggct cacccaggca
      181 agaagctctc cttcatgggc aacgagctgg cccagtgggg cgagtgggat cacgacgcct
      241 ccatcgattg ggattgcctg aactggcagg agcaccgtca ggtccagacc atggtcgccg
      301 accttaacgc cttctacaag gcgcatccgg cactatggag ccaggacttc gacccggccg
      361 gcttccagtg gctgaccagc gacgatgccg accacaacac gctgagcttc ctgcgcatcg
      421 gtaccaaggg cgagaccttg gctgtggtgg tcaacttctc cggtgaggcc tggtccgatt
      481 accaggtggc gctgcccacc ggcggcaagt ggaccgaagt gttcaccacc gatgacgcca
      541 agtacggcgg ctccgacatc cacaacggca ccttcgaagc ggtcgagggc gaatatcatt
      601 cacgtccgtt ctcggcgaag atcaccgttc cggctctggg agttgtattc ttgaagccgg
      661 aagactgaac tggtcaacag gctgaaaggt aaccggttta tttatgctca ataccacagc
      721 gcgtcagaat acgacaccgc gcacggtgct ggtggtcgag gacgagccca cgctcgccac
      781 ggccatcgcc cagcgcatca ccgctgaagg atggaccgct cgcgtggccg gggacggcgc
      841 ttccgccgtc caggccgcgt cccagctacg ccccgatctg gtgatcatgg acatcatgct
      901 gccggttatg gatggtctag aggccaccaa acgcatcgtc gccgaacgtc cggtgccggt
      961 gctgatcctc accgcacgtg atgacgaggc cgacaaggtc atcggtctgg gagcgggtgc
     1021 cgatgattac atgaccaagc cgttctccat gcgcgagctt atcgcccgct gcaaggcgct
     1081 gctgcgccgc gtggagcgtg cgaaggtcat cgccaagaac tcggagaacg agaagctgct
     1141 cgacttcggt tcgatggtca tcgacccggc ccagcgcatc gtcaccgtga acggcgaaca
     1201 ggtgcatctg acccccaccg agttcgatct gctggccacg ctggcccgcc gcccgaagtc
     1261 ggtgctcacc cgcgaaaagc tgctcgaaga ggtctgggac tgggtcgacg catccggcac
     1321 ccgcaccgtc gacagccacg tcaaggcgct gcgccataag ctcggtgccg ataccatccg
     1381 caccgtgcat ggcgtgggct acgctttcga gccgccgacc gcgtgatggc atccaaaaaa
     1441 caacagcaat cccggcttga tgcgatgaaa cctcgcgtca agccggttga tttgttctca
     1501 tctctgaagc tggaactcag cgcactgatc gttatcgcca ctgccatcgc gtttgccatg
     1561 tgctggttcc tcctgaaatt cggctggtcc ggttggatcg ccatgccgct gactttggtg
     1621 gtggccttgg gcatcaccta tttcttctct cgcggcatca ccgctcccct acgccagatg
     1681 cgcgatgtgg ccgaagccat ggcggatggc gattacacgg tccgcgtgga tatcgatcag
     1741 gatcgtcatg annnnnnnnn ncgttcaacg agatggccgg cgaactcgag catgccgata
     1801 aaatgcgccg cgacatgatc gccaatgtga gccatgagct gcgcacgccc gtttccgctt
     1861 tgcaggcgat ggtcgagaac atggccgacg gcgtcaccga gcccactccc accaatctgg
     1921 agagcatcct cacccaaacg cagcgtctct ccgatctcat cgccttccta ctggacctga
     1981 gccgtatgga ggccggtgcg gcgagtctga atatcgaaaa gttcaacttc gccgactttc
     2041 ttgacgagac catcgagccg ttggaaatcg ccgatggtgg ccacgcccac gacatccgcg
     2101 tcaccgtgcc ggccgacatc acgatggaag gcgatcagga ccgcctgcgc cagctattca
     2161 ccaacatcat cgccaacgcg ctgaaacatt cggccgacgg caccaccgtg ctggtcgacg
     2221 cccacgagga cgaaaaccaa ggcaccatcg tgaccaatgt ggtgaatttc ggctcccaga
     2281 ttgcgccttc cgaccgtttg gacatcttcc ggcgctttgt caaaggcaag acgggtcccg
     2341 gcaccgagtc gggcggcacc ggtcttggcc tgtccatcgc gcgctgggcg gcccaattgc
     2401 acggtggcac cgtcaaagtg gtcgacgaca cgcgaggtgc cgatttcgaa atcatcctgc
     2461 ctaaatatca catcgccgaa cgctgatatt gtccgacgtc ctaccgtata ctgtattcga
     2521 acagatgttc gaatacaggg tgggaggcgc agcatgacac agcaatccga gtcacaggcc
     2581 atggcgcacg aggccacgct gtttgttatg cagatggccg gcgattccat gatcaatgcg
     2641 ggtattcaca acggcgatct gctgattgtc gatcgttcgt tggcaccagt ccccggagat
     2701 gtggttgcgg ctgtcatgga cgacgaaatc gccatcaagc ggctggtttc acgggccggc
     2761 atcaccatat tgcacgccga gaatccgcga taccccgatt acatgccgag caacggcgct
     2821 tcgcctgcta tctggggcat cgtcaccgat gtcattcacc cgttgatatc gtcattggtc
     2881 cgtcgcacca cggcatccgc caataccagc acgcccacca ccttggtgcc ggcctgctga
     2941 aacgcctgaa cgcattgccg cacagtggca cctgtggtga cgatatcatc aacgagaagc
     3001 accgcgtcgc cattgccgat gagatcacgc ttcaccctga catgaccggt gatacgacgg
     3061 gcacgctgtg ccgaagagac ctgctgcacg gagcgtgcct tggtggagga gctcagggca
     3121 ttgcatggtc gcgcatcgac gccgtaccca cgcaatgccc ctgccaccga tttggccaac
     3181 ggtgccgtat gggcacgtcc tcttcggcgc attgacgccc gcgatgatgg caccggaacc
     3241 accaatacgc gatgacccaa gcatgatgat ggtattggcg tcttgttcag cagcgacacc
     3301 atgatgcggc cgaacgggcc gtcaagctcg gtatcgtcgt ggtccttcca gtcgagaatg
     3361 gcacgacgta ccgctccctg ataaatcgat gcggaccata tacgggctcc gccaccactg
     3421 tctgcatccc gttcccggca ttgcgcaaac agggcccagc atgccgcaca caatacctca
     3481 tccggcgcgt cacacccagc gcagccgcgt ggaaacagca gattgcagac ctgtgcccac
     3541 atcgcatgcc accacttgta tacgctcggc ccgtccataa cgcgtcacta tcgagctgga
     3601 gtatcgaatc gcgcaagcag tgcatccagc agttgtaccg tatcttgcgg attctcaaca
     3661 cgaacaccga tcgcaccggc ctcaaccgcg ggatagtcat tacccgtagg cgtcatgcga
     3721 tcacccacga acaccatttc atcggcccgt atgccaagtg tctgcgtcag cttgcgcacg
     3781 gcataggcct tatcgatgcc gcattcgctg acatccacac tggtgtatcc gccggccctg
     3841 gcccgcatat gcggcagatc ggccttgacc gcttcgacca gcgcctgttt cttcgtgtta
     3901 tcgcgatccc atgcctgctt ggccgctacg ggcgcgaact gcccaagagc ggagaaggtg
     3961 atctggctgc ccctgttctc gattcgaggc ccccatacct gctgttccca caatcccagt
     4021 tcccgggcat gtcgctcaag actttccgag acagcggcca cggtggcttc ggacagatca
     4081 tgcgcataca ccaaggccca ttgcgtgcca tcccaccggt aataccggga gccggaggta
     4141 ggcatgacat gcagattgcc gcgcctggca ttcggggtga gtacatccag cacctgcgag
     4201 gtgaccaccg ccatgccgcc ccctgagatc agggccacca ccgtatggtc gagcaacgca
     4261 cagaaccgtt cgatcatcgc gggcttcatc ggctgttttg agctggccag tgtgttgtcc
     4321 aaatcgaaac cgaacacctt ggtgttggag cagacattat cgaaatcaag ttcggaccac
     4381 gaacgtacca ccatttgtcg tgcctccctc gttgacgaat gttcacagcc aatgaaccaa
     4441 tcctaacgca tgttgcctcc gccatgccct atggtgggaa ccatgcttca gccaccttgg
     4501 aacgcacgaa tctaccgcaa ccgccacggt cgcggcacac gaaccccgat gttcggcacc
     4561 cgtctgcccc gttaccgcac tcgcaccggc atgttcgacg atatggtggc ggcccaggtt
     4621 cgcaggctcg gccaagcgtg gccggaactg atacgtccgc tccagttcgc ggtggaggat
     4681 gtgccgcctt cggatccgac gccttggcag accgagccga atatgacgtc ccagtgcttc
     4741 cccgccagcc acggcattcc cgcacgagtc gtgctctacc gcatgccgtt gcagaccgag
     4801 gcacctacca agctcgaact gcaattggcc gtcagggatg agctggtggc ccgcatagcg
     4861 gaattgtatg ggcgcaggcc cgaggaaatc gaccccgact ggggcctgtg acaacactgc
     4921 gggccgtgtc acttgtaata aggtagtggc acggcccgca gctatatcgc cgccgaaaca
     4981 tcatcagact gacgcttcgg cgagcatacc cttctacttg acgatggagg aatcgttccg
     5041 agcccagaca tgaaccgtgg cgggcatcag cgttgaagcg tcgatgacgg acaatccggc
     5101 gatgtccttg tcttgcaacg aatcgtgact gaaccgcgct ccccagctca ctttcttgga
     5161 tgagtcctcc aacatcagca cgaccgcggt gccatgatcg ccggaaagat tgccggcttc
     5221 aatcgtttgt gctgcgttcg catccagtgt gatttccttg gaggttaccg aagcaccggc
     5281 atcatcgtag gcgcgcaagg tgaccgtcct gcgcgcattg gacgtattgg ccagcgtgat
     5341 ggaggccttt actccgtcgg gaagggccaa ccccgagaat tcggatggct cgccggcatt
     5401 gaccaatgcg aaatcggctt ggccggagtc accggagcgt gtggccttga cggagaacga
     5461 cacggcgtcc tctgcggtgg acatcacgcc caacgcccca tcaggagctt ctcccaagtc
     5521 gatcgccgtc actttatcag cagtgacggt ggcatctttg gcgggaacca agcctttggc
     5581 ggt
//
LOCUS       DAWBMW010000098         5576 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_3233_length_5576_cov_2.830284, whole genome shotgun sequence.
ACCESSION   DAWBMW010000098 DAWBMW010000000
VERSION     DAWBMW010000098.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 5576)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5576)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5576
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3233_length_5576_cov_2.830284"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1197 a         1627 c         1645 g         1107 t
ORIGIN      
        1 tgttcatgcc gaacgtgtgg gccagcggca tcagcacgtc gttgatctgg ttcgcgagct
       61 cgcgggtggg ggccagcacc aggccgcgcg ggtgcggtag gaagtcgtcg gcgcggcgct
      121 cttccaggct ggccttcttg cggcgcttga tttccttgcg gaactcctcc atcgcgattt
      181 cgccgaacga atcgtaggaa ccgaggcggg tgaccagcgg aatggagaat gcgagggttt
      241 tgccggagcc ggtgcgacct cggccgagaa tgtcgcggcc ggccagtgaa tcgggcaggg
      301 tgtcggcctg aatggggaat gcggtcttct tgtcatctgc cgcaagcacg cgcaccagcg
      361 gtccgggtac gccaagttcg ccaaaggtaa gggggcggga ttccgcgctg tcgttcatct
      421 ggaaatcagc ggaatcgtca cgatcatcga aattattacg gggcacagtg gcctttcatg
      481 tcaaaggtta cgcaaattac gcaagctttc cgagcttagc gtaagccccc tactttgctt
      541 ggtgctcgcc acactgtcat cccactcttt acttatgacc ccatggggcg gtaactcgcc
      601 catggggcgg caacttgccc atggggcggt ttgaagaaat tgtgaaaccg cagaataaag
      661 ccattttcac ctaccgcccc atgagggttt taccacccca tgggatattt gccgccccat
      721 gggttttacc accacgtaag gcgagcaccg ctccatggaa cacgcaaatg gcccccaccc
      781 gtgaaggtag gggccattgt gcaaaagggc tgagccaaga tacagcccag tagccctaaa
      841 acacactaca aaatatccgg atcgtctttg gcaatagtgc cggttgcctt gccaatggcc
      901 ttcaggccgt ggtaggccac aagaatcacg atcgtgccaa tggcaatgcc gttgaaggaa
      961 atgccaccaa cagtgaacgc gaattggccg atggcaatga tcatggtgat ggctgccacc
     1021 atgatattca gcggcttgtc gaagttgacc ttgttctcca cccagatgcg gataccgatc
     1081 atgccgatca tgccatacag cagcgtggtc acgccaccga gcacgccggc ggggatcgtg
     1141 ttgatcacgg caccgaactt cgggcacagg ctcaggatca gggcaaaggc ggcggcgcac
     1201 cagtaggcgg cggtggagta caccttggtg gcggccatca caccgatgtt ctcgccgtag
     1261 gtggtggtgc cggagccgcc gccgaagccg gcgagcgtgg tgcccaggcc gtcggccatg
     1321 agggcggtgc cgatctgatt gtcgtagtcg cggccggtca tttgggccac ggacttgaca
     1381 tggccaacgt tctcagccac aagcacaagc acaacaggga tgaacatggg aaggatggag
     1441 aaatcggcct gcggcaggtg gaacttcggg aagccgatcc atgcggcgtc gccgatggcg
     1501 gagaagtcga cctgaccacg gatgcaggca tatacatagc cgatgatcac gccgaccaaa
     1561 atattgaggc gacccagcaa gcccttgaac agcacagcca cgagcagcac ggccaacagg
     1621 gtaaccagtg cggtgtcggg cgcggcttgg aagttggtcc acacggaagg tgccagattg
     1681 aagccgatga tggccacgat cgcgccgttg accacgggcg gcatgatgat gtcgatccat
     1741 ttggagccgg cgtaatgcac gagcacgcca accagggcca gcagaatacc ggtgaccatg
     1801 atgccgaagc tggcgaccgc aatgcccttg ttggcggtgg tgaccgcggt gatcggtgca
     1861 atgaagccga aggacgagcc caaatagctc ggcagcacgt tcctgttgat cagcagaaac
     1921 agtgcggtgg acatagccgt gaagaacagg gtggtggacg ggtcgaagcc cgtcagaatc
     1981 ggcaccagga acgtggcacc gaacatggcg atgacgtgct gtgcgccgat accggcggtg
     2041 cgcacccaag tcagacgttc atcgggctcg actacctcgc cgggcgccag cgtcttgccg
     2101 tcgccatgca attgccattg gaagatattg gacattacta ctctcctcgc tctcgcttgg
     2161 ggcacggcat tcacaaccag ccgcaccacg caccattcta gctcacgaag attttgtgaa
     2221 gtctctcttc cgggaaaggc tatttgccgt aatactccag caggtgtgga tcgacggcct
     2281 gcaccacatc caccaaatcg ccattggaga gatacgggcg cttgaattcg cgccacggct
     2341 ccaccttgac ctcccagcgg ccggtcgtct cgtcgagcac gggccgggcg gtctgctccc
     2401 agcgcacacg cttggaggtg cggccggttt ccgggtcgcg gcgcgtgtag tgcaggcagg
     2461 tgcagttggt gatcttccag tcgtccgagt cggcgcgatg caggaattcc tcgtcggcca
     2521 gatcctcgat ggtgagcatc agggcgagca tgaagtcgcc gtgggtgacc atgaccacgg
     2581 attccgaatc ggatttgcgg ctcagcgagg tcaggatgtt gtgcacacgg tcctcggcca
     2641 catcggcaat cgactcgcct gccggcggac gccagtacag cggatcggta ttcttgaagt
     2701 tccagttgcg tgcgtagttg tttttgaatt cgtccttggt gatggtgttg atttcacccc
     2761 aagaacgttc gcgcagcaca cggttctcct cccatttggc cttgggcagg gccatggtgg
     2821 ctgcggtttc gcgggtgcgc acgtatggcg agaccatgta acggtcgaac agctgctgct
     2881 ggctgaccag ccaacgaccg atgcagtcgg cctgcttgcg tccggtggcg gtgagtctcc
     2941 aggagcggtc gggtacggtg acgttgtcct gcgtatacag ggagttgtcg ccctgttcgc
     3001 ccgcctggac gatgacgttc gcttcggatt cgccatgccg gattacgtat agatcgagcg
     3061 gcatgctcat atggattcct ctttcgttat tggttacgtt gtgttgtttt ctgctgtttc
     3121 ctacttgacc tgcgtaatgg tgttcccctt gaccatgaac gcatgccggg ccatgtcgat
     3181 catcggctga tcgaacttgg agtcggtgta gaactcgtcg atgacggcgt ccggaccgta
     3241 cagttcgcgg aaccgtttgg tcttgttggt gttgaagttg agggtacgtg accttcatcg
     3301 tctccacatc cacctcgctg gccacgagat tcctcacgcc gagtctgcgg caggcctccc
     3361 ccaccgtcag accgaaggat gcggtgagaa tcacatcgtc cgactcaggc tggtaccaag
     3421 gcttgatacg tgcgtaatat cggtcccaga aatcgtccac gagctgttcg acgctcgcca
     3481 cccgtttgga tcggctcagt tccgtgagat agccttcggt catcttgccc acgccgtatt
     3541 ccattctcgc agacgcaagc actgaatatg gcattcccga atcagactgt atatgacgcc
     3601 ggtcaagcag gcgaaggcac cgccccagaa acggttgatt ttactgtgct caatccggat
     3661 acatacaacg tcgccaaggc tcagggaacc gccgccttcc ccatcagtgg aatatccaaa
     3721 atagacaaca gggacggcgg cacgacgttc aatggcgagg tccgcgccgt ggcagatgga
     3781 ttcaagccat ctgatggaca gcaaatcaag atttccctag tactgcgtaa cgcacagaac
     3841 gcaatcatct atggagatat cgcattcgtg gattggccgg gcaatggccg atccacgcca
     3901 ttctcgatta ccgtctacga tctgccgaag tatgtgtctt acgatctgta tgcgcaaatc
     3961 tggtagttga ctttacgtct ggcaggtggc cgtcaggtgc ctgtcaggcc gctgttcgcg
     4021 tcacctgcgc actgacgtta cggcgcgcct ggccgaacag atagatgccg atgggcaggg
     4081 tcagaacacc gccgatgatg aacaccagcg aaatcgggat cacgtcggcc agtggcgcga
     4141 agatcaacat gccgagcggc attccgagcg tgctcagggt ggtgtcaagg ccgaatacac
     4201 ggcccaccat cttctcatcg gcctcggtct ggatgatggt gcgcatcggg cccatgcaga
     4261 tggccgaagc caaaccgttc accacatcaa tcagcaaata cgccagcaga ttgtgaacca
     4321 cgccaagacc aacgacggag acgcccacca gagcgatgcc gaaggcgacc acgcgcatgt
     4381 tgttgcggat gagcttggca ccgatggtag acatcagcgc gccgccgagc agcataccga
     4441 tgctccatcc cagctcgttg gcggcgagtt tatcggaggc ggtggtcagg ttgatgaatc
     4501 cgagattcaa atcgatgccc tcatattcgc gggtcatgag caacagcgta aggttcatcg
     4561 gcgccacggc cacgaagcac accaaggcgt caccgagcac cacgttgcgc aggttcgccc
     4621 ggcgccatgt gtagctgaaa ccatctttca agtcagcaaa cacacgacgg acgctggcaa
     4681 tcagcgaacg atcagcattg gcatccgaca taggttcgag tgtgggttgc ccgctattct
     4741 ccgcttcagc catacgcctc gcaacatgtt ccgcgctctt cttcggcgct ttgatcaacg
     4801 ccacaaagcc gacgccgata atggccgtgg tcacatcgac taacagaatc gaccacagcg
     4861 gcactgtgtt gataagcaca gcagccaccg caggtgccac aatcatattc gccgaattaa
     4921 tcacgccgta tattgagttg acgcgcaata gtttgcccga gggaacaaca tccggtatga
     4981 acgactgcac agcgggcgtc tgcaccccgc ctcccgccga acgaatgaac agcaccacga
     5041 aaatcatggt cagactcgcc catcccatcg cgaagctcag cgacagcacg acggtgacca
     5101 cagcaatcac catatccggc agcataatca gcaacttgcg actgtgacgg tcggcccagg
     5161 agccgccgaa gatcgagacc acggcctgcg gcaccacgcc gaacagggtg gccagcagca
     5221 tcgccgaacc ggttttagtc tgcaatacga tccaccacca gatggcgtac tgcacgatgc
     5281 tggagcccag cagcgagaac gcctggccgg tgagtagaag cactacacga cggatccacg
     5341 cacgctcgga ttccccgttg gccactgctg cggcataggc ggcaccgggt ttgttcgcgc
     5401 tatggaccgc tgcgcgcgca ttcacattgt catcgccgga aacctcgggt gtttcgcgca
     5461 cagttggttc agttgttgta ctactagaag acatacgagt gttctccatt cttcaattcc
     5521 gctacaaagg tcacatcacg gcatacgcac gccgacgcgc gtatgcgtgc acgact
//
LOCUS       DAWBMW010000099         5561 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_3241_length_5561_cov_2.871231, whole genome shotgun sequence.
ACCESSION   DAWBMW010000099 DAWBMW010000000
VERSION     DAWBMW010000099.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 5561)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5561)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5561
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3241_length_5561_cov_2.871231"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    857..866
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1151..1160
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4639..4648
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5227..5236
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1116 a         1738 c         1563 g         1104 t
ORIGIN      
        1 tggtggaatc gactgccttg atgaagtcct cttcggtgcc gatgtcgttg atggcaacct
       61 tggtgacttc agtgttgttc tctgccatgt aaatatatgg tttctaatag tggatggata
      121 atttctcgat attcagttat acgcacaccc tacaggcata cagatactca caatacaagc
      181 aactctgccc aaatttccta ggaaactcta ggcgtgtcgt tcggccattt ccacgacgtt
      241 ggctaggagc atggctcggg tcatggggcc cacgcctccg gggttggggg tgtaggcgga
      301 ggccttgtcg tagcaggcct tgtccacgtc gcccttgatg cggtagcgac cggcttcttc
      361 gtcgtagacg cggctgacgc ccacgtcgac cagcacggcg ccgtccttga ttttgtcggg
      421 cgtcacgaat ccggccgaac ccattgcggc aacgatcacg tcggcgcggc gcatgtggtc
      481 ggccacatcc cgggtaccgg tgtggcacag ggtgacggtg gcgttgacgg cgttgcgcgt
      541 gagcattagg ccaatcgtgc ggccaatggt gatgccgcgg ccaagcacgc acacttcctt
      601 gccgttcaga tcaatgtcat aggcgtcgag tagctcgagc acaccgcgcg gagtgcaagg
      661 cagcggcgtc gaaatgtcgc cgcgcacatg cagcaccagt tcgccgaggt tgtacgggtg
      721 catgccgtcg gcatccttgg ccgggtcgat catgtcgatg atcgcgttct cgttgatgcc
      781 cttgggcatc ggcagctgga cgatgaaccc ggtgcaagcc ggatcttcgt tgagttcgcg
      841 tacggcagcg gcgatgnnnn nnnnnnccca ccagcagcgt gcccagcccc ggttggatgc
      901 cctgcgcctt gagcttgttc actcgctcgg caaggttggt cttgatttcg gccgcaatct
      961 gcttaccgtc gatttttgtg gacatcaacg ccctcccctg tagtttcggt gcattctatc
     1021 gaaacgatag gctatcgttg gaacaagtca ggattatata tcgttcgctc tcacgaagaa
     1081 ggcgtgccat ggggactcaa atcacacgac ttacgcgact cgccgcagcc ggcctgagcg
     1141 ccgtcctgtt nnnnnnnnnn gttcgcgacc accgcatgcg cagccacctc ccccgctgaa
     1201 accgagccgg acaacaagac cagcgttccc gcaccgaccg ggccgatcaa tgtggtggcc
     1261 tccctcaatc aatggggctc gctggccgcc gagctgggcg gcgatgatgt ggaagtcacg
     1321 tccatcgtga actccaccaa tgtggatgcc cacgatttcg agccaaagac ctcggatgtc
     1381 gccaagctgt ccaaggccca aatcatcgtg gccaacggtg cgggctacga ttcctgggcc
     1441 accaaatcga tgaccaagac cacgaatatc gtatccgccg cttccgtcat gggtgccgtc
     1501 gagggcgata acccgcatct gtggttctcc aaagacgctc gatccagcat ggccacggcc
     1561 atcaccgatg catatataaa ggcactcccc agcaagaaga aagccttcca gaagcggctc
     1621 aaggcatggc aggcggacga gaaatcgttg gaaacatggg cgtccgagtt caccaagtcc
     1681 cactccaacc tcacctatgc agccaccgaa cccgtggtct attatctgat ggccgatctt
     1741 gggttcaaag atcagacgcc caaggggtat acgcaatcga ccgcttccgg cggcgaaccg
     1801 gcacccgcgg acctgcagtc tttccagaaa ctcatcgaag acaagggcgt ggatgtgctg
     1861 gtcaacaaca cgcaggaagc cagtgacgcg accaatatga tcactggcgc tgccggtcgc
     1921 gccgatgtgc cggtagtcga cgtctccgaa caaatgccca aggacgccga cacgctgaac
     1981 gcctggatca accagctcat caacaccatc atcgacgcgg tggatccaag ctatggctgc
     2041 gaagacgcca cggacggcga taccgcggac agcaacgcga attccgatga ttccagcgcc
     2101 gaaggctccg ccacggatga tgccaatgcg caagactcca aggccagcac cggttccagc
     2161 acggacccca agtacatccg tcagtgcaag gcggccgcat ccagtgactc atctcaggat
     2221 tcggcaaagg ccgatgccgc caatggcgat cccggcgatg cctccggcaa cgaccagacc
     2281 gactccggca aatagttagg agcaaaggac accggcatcg gattcgcgcc aatgccaata
     2341 gacgccttgc atgagaaggg ttgtgcgacc attgtcgaga tggccgcaca acccttctct
     2401 ttggttatca ccatccgtac acgatattga gaacgattgt cagcatcgtt tgccacaatc
     2461 gggcgttatg agtgaaactt ccgattgcat cgtgttcagc gatgccgcca tcaagcgtgg
     2521 cgaccatatc atctggcagc atggcacctt cgccattccc accggttcgg tcacggccat
     2581 cgtcggcacc aacggcaccg gcaaaaccac gatgatgagg gccgagctgg gccttttgcc
     2641 gctctgtcat ggcagcctga ccgtgctggg caagcccgca ggcgagatga acaaacgcat
     2701 cggatatgtg ccgcagagct atgtggcgaa tgtcgattcc aatctcaccg ccgaacaatc
     2761 cgtgttgctg ggcctgaccg gcacccgatt cggcatccac cccatcacca aggcccagaa
     2821 agccaaagcg ctcgaggcga tggagttcac cggcatcgcc gacaaggcgc ggtaccgtct
     2881 gtccgaactg tccggcggtt tgcgccagcg tgtggccatc gcccaggcgt tggtgtgcga
     2941 cccacaactg ctgatgcttg acgagccgtt ggccaacctc gatctggcca gtcagcgcgc
     3001 caccgtacac gtgctggcca agctgaaccg cgaactgaac atgaccatcc aggtggttgc
     3061 gcacgatctc aatatgctct tgccgattct gaccggtgcc gtctacttgc tcgacggtca
     3121 cccgcactac gccgatatgc acaaagtgct cgactccgac ctactcaccc acctctacgg
     3181 cacacaggtg caggtggtca ccactccgca gggagacatg ttcgtgaccc cgacgccgga
     3241 tgagctccag gaccagccgc aggatatgca cacggcagcc gaagtggcgc aattgcacca
     3301 tcacgagcac ggcaatgcca ccaatggcag tgccgccatc agcgccgaga atcgataaca
     3361 tcaagcgagc cgacccacag cccatcaacg gactgcagct caggcatgga gaaaatacag
     3421 agacatgagc accatcaact tttcgtatga tcccgaatgg ctggatacac tgtccgctcc
     3481 attcatgacc aacgccttca tcgccggcct gtgcattgcc ctagccgccg gtgtcatggg
     3541 atacttcacc atcgcccgtc gttccacctt cgcggcccat gcgctggccc atatcggtct
     3601 gccgggcgcg accggcgcgg tgttgctcgg cctgcccgtg tcactgggca tgggcgtgtt
     3661 cgcactgggc ggcgcactgg tgatcggtgc tttgggcaag cgcgtttccg agcgtgaaat
     3721 cgccaccggc accgtgctgg ccttcgccac cggcttgggt ctgttcttcg cgcgattgtc
     3781 gagttccgcc tcccagcaga tgcagtcgat cctcttcggc tcgatcctga ccatcaccac
     3841 cggccagatc atcggcttcg ccgtgttcga cgtactgctg ctggcattgt tggccatcat
     3901 ctaccgcccg ctgctgttca gctcgctgga tgagcaggtg gcacaggcca agggtgtgcc
     3961 gatcgggctg atgaacgtgg cgtttatggc gattatggcc ggtgtgatca ccatcgccgt
     4021 gccggcagtg ggcaccttgc tgattttcgc actggtgatt acgcccgcgg ccaccgccaa
     4081 tattctggcc ggctccccat tcaaggcaat ggtgatcgcc agcgtgctgt gcttggtgtc
     4141 gatttgggcc ggactcgtgc tgtcggcgat gttccccgct cccccgagct tcatcatcgt
     4201 gacgctttcc accctgttct gggctatcgc caaagccgtg gagacgatgc ggaagtaatc
     4261 tcatccattt tttcttgccc ccgctggcga attcatagcg ggggctttgt tatatcttct
     4321 ccaccaaggc attggcgatg gcggcgatgc cttcgccgcg gccagtgaat cccatatggt
     4381 cggtggtggt ggcagtaacc gataccgggc aaccaaccgc cgcagacagg gcggcttcgg
     4441 cttcagcacg gcgtgtgccg attttcgggc ggttgccgat gatcgccacc gaggccgatg
     4501 ccggcgtata cccgttggat gccagatgcg ccacaacttc ttgcaacata tcgatgccat
     4561 gcattccggc accatgcgcg tccgcaccga caccgaacag tgaaccaatg tctcccaagc
     4621 gggcggcggc aagcaacgnn nnnnnnnnat cagagcgtgc gcggccacat cgccatcgga
     4681 atcgccttcg atcccctcgt atttggcggc atcggcctcg ctgcatgaat cagacgccgg
     4741 ccagtagagc ccggcaatcc acagttcacg gcctgagccg gcaggcgcaa aacggtgagc
     4801 gtcaaaaccc tgccctatca acactcctgc agaccccatg cagtcccctt cgatggcgct
     4861 gcccagacga acggcagcaa acgattatga atcacgaagc cccggcgtat cgcataagga
     4921 cacgaccggg gcctcaacaa tcaaatcact tcttcttgga cttcttctcg gcttcgacac
     4981 gagccaaagt atcggccgcg gcctcttcgg gctcggcggt gtgatgcttg tcgtcaccgg
     5041 gttgagcggg ctcgtagccc aggttcacgt cgagcagacg ctgggcttcc gcctcgtcga
     5101 tcttctcgga caaggcgatc tcagaagtca gaatggcgcg ggccttggtg agcatacgct
     5161 tctcccccgc ggacaggccg tgctcgtcga catcgcgctg ggcgagatcg cgaaccactt
     5221 cggcgannnn nnnnnncggc gatcttgttg acatcgccgg tggcgatctt ttcgacgttc
     5281 agcttgtaac ggcgagacca gttcatctct ttctcgatga tcggggtccg caggatgccg
     5341 aataccttgg ccacttcgga tgcggagacg atgtcacgca cgccgacctt cttggcattg
     5401 tccacgggaa cgttgatgac caaaccatcg gaagagagta cggacaactg cagatattcg
     5461 cgcgtcactc ccttgaccgt ccgctcggta atggcctcca ccttcgctgc accatgacgc
     5521 ggatagacga ccatatcgcc gactttgtaa gacatgtacc c
//
LOCUS       DAWBMW010000100         5513 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_3270_length_5513_cov_3.300293, whole genome shotgun sequence.
ACCESSION   DAWBMW010000100 DAWBMW010000000
VERSION     DAWBMW010000100.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 5513)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5513)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5513
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3270_length_5513_cov_3.300293"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4016..4025
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1197 a         1682 c         1562 g         1062 t
ORIGIN      
        1 tggcgcagat catgccgtaa tcgaagtgct tggagagcac cagatcgttg gcggcacgca
       61 cgatgtccac atcggaatca acataggccg gggcgttgcc ggcacccacg ccgagggcgg
      121 gcttgccgga ggagtaagcg gccttgacca tgcccggacc accggtggcg aggatcgtgg
      181 ccacgcccgg gtgctgcatc agcgcgccgg tggcctggat ggacgggtgc tcgatccact
      241 ggatacagtt ctcgggagca ccggcctcga tggcggcgtc gcgcacaatc ttggcggcct
      301 cgacggagca cttctgcgcg ccggggtgga agccgaagac gatcgggcag cgtgtcttca
      361 gggcgatgag ggacttgaag atggcggtgg aggtcgggtt ggtaaccggg gtgacaccgg
      421 cgaccacgcc gacgggctcg gcgacctcat cgatgcccat cacatcgtct tcgcggatga
      481 tgccgacggt cttctggccg gccaagtagt tggtgacgtg ctcacaggcg aagatgttct
      541 tggtggcctt gtcttccacg agaccacggc cggtctcgtc gacagccatc ttggcgagaa
      601 cgaggtgctt gttcagagcg gcgatggagg ccttggcgac gatacgatca acttgcttct
      661 ggtcgaggtt ctcgaattcg acgagagcct tcttggcgcg ggcaaccagc gcgtcaactt
      721 cggcctcagc tgcggcctgc ttctcttctg gagtcggctt ggtagcttcg accttcttct
      781 ttgcttctgc cacttggggt cctcctcgat cttcttgggg tatatggcaa agttctgggc
      841 catcgcctcg tcacctcagt tgcaccagcg ggataccgcc tcagtaaccg aaaatgaccg
      901 gtgatgcgtt gtcacattgc atatgtgacc tgaggcacaa tatactgaac ggtccggcgc
      961 gatgcatacg cacaaaatcg acggcgtttc acgtgcgata ttgaacagtt tttgttacga
     1021 gttgtgcgaa atatcgaaca aatcctcact ctatccggcg atttggtact ttttgtttac
     1081 tttttaccga aaatcacatg cctgcaaaca acacaatccg agaacttttt taaagtaagt
     1141 tttcaaaagc aatcattttc tccacatttc tagaaagttg gtttaaaaaa gtacccaaaa
     1201 tcgcctacaa atatacgatt gttgcctcat aacctccgcg tcgaggcaca tgaaagccac
     1261 tttcataaag taaacttgca tatatctcat cgcacaaagg ggtaaacatg agggcaatgc
     1321 catcgaccgc agtgaaagcg cacaaccgca gtgccatggc ccagtatctg tatggccata
     1381 gccatgtcac caagcaggaa ctggaacgcg aactcggcct cagcctgccc accatcaccc
     1441 aaaatctgca cgcactagaa gatgacggac tcatccgcaa aggcgaactg caggattcca
     1501 ccggcgggcg caaggcgcaa aattatgagt tcaatcccga tcatcgcact gccatcgggg
     1561 tagtgatgcg gtccaacgaa ctgcgcctgt gcgcgattaa cctgtacggc aacgtcatcg
     1621 aaaaacgcaa cgatgccctg ccttaccgca acaccaacgt ttactaccag cgtatcggcg
     1681 gcatcatcaa caacttcgcc gccgacgtcg aaaagaagca cggcaaggtg ctcggcgtct
     1741 cgttcgccat ccaaggcatc ctctcccccg acgccaccac catcatcttc ggcaccatta
     1801 tgggcaacac tgggctcacg ctcgaaacca tcagccaatc ggtccattac ccgtgcatga
     1861 tgatccacga ttccgatgcc tcagccatgg ccgaactctg gttcgactcc acgctcaccg
     1921 atgcggtgtg cgtctatctg gagcgacgcc cgggcggcgc ggtgatcgcg gggggcaaac
     1981 tgcatcaagg cccgaaccag tgcaacggcg ccatcgaaca catgacgctg gtgccgggcg
     2041 gccgcgaatg ctactgcggc cagcgcggtt gcatggacac ctactgctcg ccggagacct
     2101 tgcccgagga ttacgaaagc attcccggct tcttcagcgt gcttgagcag ggcgaacggc
     2161 atcatcgcga gcgcatggat gagtggctgg attacgtggc gcaggccatc gtcaacgcgc
     2221 gatccatcat cgcgggcgac gtgattgtcg gcggtgaagc agcccagcat cttgaagaca
     2281 gtgacatcga ggatctgaag gcgcgcgtca tcgcacgcag tccgttcggc accgaccatt
     2341 tcaatctgcg caagagctac tgcgccgaag atcagaacat catcggtgcc gcattgcggt
     2401 tcgccgagaa ttatctcgac ggcatctgtg gtgctgcgga acgcggcagg aaatcaaggg
     2461 cttccaccag aagtgacaac acgtctacaa tggaggtatg aaaaacccac cgttgcatcg
     2521 ttacgccact cgcccagggc tgtacttcac tgacgacggt ggggctgacg tcgttgttcg
     2581 ttccgaaact gccgatcagg tttggctgtg tgtgctggag cccattgacg agcccagcgc
     2641 tttcttccaa gacgccatcc gcctgttcga ggacccgaat atctccttta tccagcagat
     2701 tcatgagttc ccggtatgca cacgcattat cgaacatttg tatctgcgcg agaccttgtt
     2761 ccgcatgacc gggccgaact acggcctgtg gtacgtgcac ctgcccaaag catgggacgg
     2821 catgcgttat ggctaccgcg tggacggcgc atgggacccg aaacacggcg tgcgcttcaa
     2881 tccatacaaa ttcctgcttg acccgtatgg caagggcatc gacggctcca tggagctcac
     2941 ccccgccgcc ttctcctatg aatgcgatgt cgtggaccgc aaggtaaccg gttcggcata
     3001 cggtgccatg agcaccgtag actcgctcgg ccatgtgccg gtgtccgtgg ccatcgacga
     3061 tcgcgatatc cataagcatg agggcgaccc gcagcacccg catgtgccgt ggcgcaaaac
     3121 cgtcatctat gagatgcatg tcaaaggctt caccgccaac gcgccgtggc tgcccgaagc
     3181 gttgcgcggc acctacgccg gactcgccca ccccacaacg ctcgcctatc tgcagggatt
     3241 gggcatcacc tccatcgaac tgctgccgat tatggccaag caggacgagc tcttcctgca
     3301 agaacacggc cgcaagaact actggggcta ctccaccctc agctacttcg cccccgaacc
     3361 ctcctatgcc accaaagcag cccaagagaa gggcgcggcc gccgtgcgcc aagaagtcat
     3421 agacatggtg cgcgcactgc acgaggccgg cttcgaagtc atcatggacg tggtctacaa
     3481 ccacacatgc gaaggcggcg tcgaaggccc gacggtctgc tggcgcggcc tcgacgatct
     3541 ggcctactat cgccatcaaa aatccaatac cggtcgactc gaagacacca ccggctgcgg
     3601 caatacgctg gacttcacca acacgcatgt ggtcaccttt gccatcgact cattacgata
     3661 ctgggccaag cgtatcggca tagacggttt ccgcttcgac cttggcgtca cgctggcccg
     3721 cctggaaggc gaattcaccc atcatcaccc gttcctctac gccctgcgtt ccgatctgct
     3781 gctgggcaac ctgaagctca ttatggagcc gtgggatctg ggcaacctcg gctggcgcac
     3841 cggccaattc tccgtgccgt tcgccgaatg gaatgaccgc ttccgcgaca cggcccgcac
     3901 cttctggctt gaggatgtgg acggcggctc cgatttcggg cgtatcagtt tgcaggagat
     3961 gtccacgcga ctctgcggct cggccgatct gttcgccacc gaaccagggc gtggcnnnnn
     4021 nnnnngcgtg gcgctccggc ctccatcaat ttcgtgtcct gccacgacgg tttcacgctg
     4081 acggacctca ccagataccg ctccaagcac aatgaagcca acggcgaaaa caataatgat
     4141 ggctcgtcgg tgaaccattc cgccaatttc ggcgtcgaag gcgtcaccga tgatccagac
     4201 gtcattgcgg cacgcgaaca ggcggccatg aacatgatcg gcatgctgct gctctcgctg
     4261 ggtacgccga tgatgctcgc cggcgacgaa ttccgcaaca cgcaggacgg caacaacaac
     4321 gcctactgcc aagacaacga catcacgtgg ctgaagtggg attggatgta ttccaccaac
     4381 aagacgcgcg aaatgcgccg cttggaaacc gtgtctcgac tggtggcgct acgtaaatca
     4441 ctggacctgt atcaccacga ggacttcttc acccgactga cccagctcgg cctactcaag
     4501 ccctcaagcc gcgtgcagtg gttcctgccc gacggcacca cgccgatgga gcgtgactgg
     4561 ttcgatctgg gcgtacgtag cttcaccatg cgattgctgt ccaacagtga ggtggacgtg
     4621 tgcattgtgg tcaacggcac cgctgacgac cgcacattcc gactgccgcc agacactcac
     4681 tggacgccta aatggtgctc ggccgaaatc aacggccgtc gagccgggca tggcactcag
     4741 gtcgaggaat gcgatttgaa cggtgacacc accgtctgga cgcagcatgt accggacgcc
     4801 agcgagacag tactcaagat ggtcaaagaa gtggccatgc agcgtacgga atcctcgact
     4861 gagaatgaag cggataccat caaattcgcg atgccagtaa cagatcacgc cgcaaacggc
     4921 acctcgtccg cgcctcggga tgaggtatca gctgatatgc ccgatgcccc tgtggatgat
     4981 acaccggaca ctcccgtcga cgacaatgtc tggaccatgc cggcgctgag catcacgctg
     5041 atgaagcagg tgtgagtcac actcaagaca gcgcgacaca aacacaaagg ctctccgcgg
     5101 aattccgcgg agagcctttg tatgctcggt acaggacaag cgaaattaac gcttggagaa
     5161 ctgcggggcg cgacgtgcct tgtgcagacc agccttcttg cgctccacga cgcgagcgtc
     5221 gcgggtcagg aagccagcct tcttcagggc ggcgcggttg gcatcacgat cgatggcgtt
     5281 cagagcgcgg gcaacgccga ggcggatggc gccggcctgg ccggtggtgc cgccgccgtc
     5341 aacgaggacg atagcgtcga acttgccttc gagcttgagc agaacgatcg gggagttgac
     5401 ctcacgctgc agcagcttgg aggggaagta ctcctccaga gtgcggccgt tgatggtcca
     5461 cttgccgtca cccggaacca gacggacacg ggcgacagcc tccttgcggc gac
//
LOCUS       DAWBMW010000101         5456 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_3305_length_5456_cov_2.496945, whole genome shotgun sequence.
ACCESSION   DAWBMW010000101 DAWBMW010000000
VERSION     DAWBMW010000101.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 5456)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5456)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5456
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3305_length_5456_cov_2.496945"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1130 a         1464 c         1671 g         1191 t
ORIGIN      
        1 caccctgata gccggccagc cgggagccga cgaacagggt aatcgccagc atcacaccct
       61 gcctgctgat cgacagcaca aaggcgccga ccgccttgcc tgcggactgg aaggtgcagg
      121 tcgtgaccat cacgacggct acgcacacca ggctcagcag ctgaacccgc agcatgccgg
      181 cgcccagcgt gatgatggtg gcgtcctcga tgaagaatcc catcagtaga cggtcggaca
      241 ggctgagcac cacggtcatc accagggcga acagggacag gaacaggtag gaaaaacgca
      301 gaatgccgcg cagtcgaggc atattgcggg cgccgtagtt gtagccgatc agcggctgcg
      361 cgccgaacgc gaatcccacg aaaaccagca ccgcgatcat gatgactttc agcgcgatgc
      421 ccatcgcggc taccgcgtcg gtaccgtagg ggagcaggaa caggttgatc atgacgatgc
      481 cgacgctctg cgtgaggttc gtgatcgccg aggggatgcc gatggcgagg atgctcccga
      541 cctcccgggc cgtgaccgcc agacgccggc gcgcgcggtc gatgacggtc cgcgggtcga
      601 tggacaggtt gctgctgtga cgcagcgtga accagacgaa ataggcgtcg gtgcagatgt
      661 tggcgatcac ggtggcaccg gccgaacccg cagcacccca gccgaatccg tggatgaaca
      721 ggggattgag cacgatgttg accgccgtgc cggccaccga accgatcatg gactgcacgg
      781 cgtggccttc ggtgcgcagc aggttggacg gcgtcatcga cacgatgatg aacggcgagc
      841 cgacgatgat cagcgtgtag aagtcggagg catactgcca agtgtcgtca gtggcgccga
      901 gcagcgcgag catcgggtcg cggaacgcca ggcccagcac cagaatcagc aggccggtgg
      961 cgatggcggc atagaagcag aacgcgctga gtcgcttgcc gtcggcgaac cggtgttggc
     1021 cgaacaagcg cgagatgacc gaactgccgc cgaggccgaa gatgtcgccg agggcgatca
     1081 tcacggtgaa cactggcgcg gtgagggaga cgcccgccac catatcggtg tttcccgtat
     1141 gcgcgatgaa ataggtgtcg accatgttgt acaccaaggt gatcagcatg ctgagcacca
     1201 cgggcatcgc cagcgagaaa tacgcctccg gaatcggcgc cttttcgaag agttcgttat
     1261 ccatttccgt cccttggtgt ttcgttcttc agtcactgtg caggatgccg ttcggcgtgc
     1321 atcacaggca ttgtagaggc aacagtgtgg acaacgggcg ggggagtatc tcatgatgcg
     1381 gtgagggcgt tgatgatgac gcatcgtgta ttggcggccg tgtgcatcgt atgtgaacct
     1441 gtgctggcgc ggcgtacgca cgatgggaaa agaaagaata tatggacccc gttgtcccgg
     1501 ttattgttca gttttactgt gttgaattat tgcgttattc aattattgtg gtgtgccggc
     1561 cgattgcggc ctgcggggga ttgccttcgt atgtgtcgtg caatctcatg aacgggattg
     1621 ttcgctagat tcagcgagcg tccttgttgg cggcgttggt gatgtcggcg aacatcttgg
     1681 agtcggcgta gccggcgcgg gcgatggcat ccataccgga gagggcgaga ccgcgaatca
     1741 tggaatcctt gagagacata atcattacct ttcgtttgcg catcgccgtg agcggctttg
     1801 cgattgttgt ttaagctttg ttgttatggg gtccgtggct aatctgattg gattgccgga
     1861 ccggagccga tggctcagcg agcgttcttg ttggcttcgt tgatcaggtc gacgagcgtg
     1921 ctgctgttgg cgcgaccgca ctggctcatg gagctcaggc cgtacacagc cagaccgcga
     1981 atcatggaat ccttgagaga catcatgttt cctttcgttg gtcaaaacct tggggctttg
     2041 agttttgttt atcggcttct ttctgaaacc ggtttcagaa taacgcagtc cgatgacaaa
     2101 cgcaagggga ctattccgct ctcggcgttt ctggcggaat atcgccgttc tttagatacc
     2161 attcggtagt taagaggttg tctagtcgac cttctgacgg ccttgttctg agcgtacggt
     2221 tttcacgagg tatggcctca tgtcgcggaa atcgtacgct gagtcgtcgt aagcgtacgg
     2281 ttttcacgaa atggggccta tcttcaccgg attttgggca caccggcctt gagcagcttg
     2341 gtctcaagat tgatggggcg tagcagatcc tcatagaaca catggacgat ttcggtgacg
     2401 ccctgttccc ggagatgccg atcacgtctg cggtcgtact ccattttcgc ctggaggctt
     2461 gcgcgattct tgttgctgat gtcggcgtat ttggccatgc cgtcgtattc tgccacgatg
     2521 atgcggccgt ctgcgagctt ccaacagaaa tcgacgcgat atgacattgc gggattgtcg
     2581 ggattcatga actgcacctg cagcagggga atgccgaaag acaattcggt gatctggcca
     2641 cgcatcaatg attcgccgcc gttttcgctc aggggatccg cgtacttcag cagcttggtc
     2701 acggagacct cgtcgcagac gctttgaatc atcagcgact gcacctcttc gatggtcgtc
     2761 agggactttc tcagagccga atcgtatatg ggcagcgcct gtgggaatgg gaatgccgcg
     2821 cagtcgagca gcgttcgcgc cggagtcgtc aagagaatgc cgttatgctt ccgcgtcgga
     2881 atccgattga tatagatgcg gttgagacgc gcctcgtctc cgccgccgat gccggacttc
     2941 gaggcgatgt gcactgtgcc gtcgtgcagg gaatagttgt gctgcagtcc gtatacgcac
     3001 gccgcactca accccgcaaa tacccactga gggtggattg cggacaacgc acgtatgacc
     3061 tgcaatgact gttgctctct ggtcatggtg ttccaaagtg ttcggtcggc gtacaggttc
     3121 ttatacggtg acaccagctc cccggcatgc aggcgacgtt tcagggcgcg ccgttgcgca
     3181 tcactggtgc catagcagca gcgacgctcc tgcttcgcct gctgcagcag tgcagaaact
     3241 tccttgtgtt ctttcatatc tcgacgctaa gggtgatttt gatcgttgca caagctcaac
     3301 cggcaaatgt ggatactatg tcacgcgaat tgttgctgtg gataacttga atcgacgtcg
     3361 acggctttgc cgacggtctt gtcggaggcg atattagcct aggacacctt gctgcgccgc
     3421 cttccatacc gaaaacagtc atcagtgaca ccgggggcat cttcagcgta cgttcttcac
     3481 gaaatcagct gccatttcgt gaaaaccgta cgctcagaat gactcagtgt acgttcttca
     3541 cgagatgagg ccccatttcg tgaaaaccgt acgttgacga cgattcagtg tacgttcttc
     3601 acgaaattag ggatgatttc gcggaaaccg tacactcacc gcaactcagc gtacggtttt
     3661 cacgaagtta tcgatttggg tataggttcc ccgtcatgaa ctacaatgaa tcgcggcgaa
     3721 aaacggagaa ccgaagtcgg agcgcggctg aacggatatg ggtgtgactg gatggctgtc
     3781 atagtgatgc cggttctcta acatacgcaa taacaacaac tgacaacaac aggggagaca
     3841 aggatggcga gaagtgcggg ccgcacccgg ccggtgatca aggacgtggc caaactggcc
     3901 ggagtctcgg caccgaccgt ctcccggtat ctcaacggca ccgtcaacgt caccgaagac
     3961 aagcgtgaac gcatcgccaa ggccatcaag gagctgggct acgagccgag cattgtggcg
     4021 cgcgctctgt cgaaccggga gatggactcc gtggtggtgt tcgcggccaa tccttccgcc
     4081 ttctccacgt ccgaaacgaa ccatggggtg gaggccgccg cccgtaaacg tcggttcctg
     4141 ctcaatattg tttctttgga cgagtccgac atggactccg tcaaggctcg cgtgcgcatg
     4201 ggtttgtcga tcaggccggc gggcgtgatc gtctacgagt acgaccgggc cggcatcgcc
     4261 gcactggaat acattcccaa agatatgccg atggtcgtgg tgggtggcag cttcggcgac
     4321 ggtgaatacc aggtcatcaa cgccgaaagg gatggcggcc gttggatgac catgcatctg
     4381 ctggatctcg gccatcgcac cgtgttccat gtggggcgtc cggaaggtcc gaccgataac
     4441 acccgcacca atgggtggcg tgacgcgctc aaggagcgcg gcgtcacggc acctgaccca
     4501 atcatcgtgc ccgacgatga tttggaggag tcggtcaagg ctggacgcac cttgggccgt
     4561 cgtggtgatg tgactgcgat tttcgccggc aacgacgaga ctgccatcgc tgtgatccgg
     4621 ggtctaaggg aggttggtcg ccgcgtgcct gctgacgtga gcgtcgtagg ctttgacgac
     4681 cgcaacttcg gtgccatgtg gaatccggcg ctgaccagtt acacccagaa cttcctggac
     4741 atgggggagc gatccttccg aatgctgttc gaacagatcc aggccaagcg cgccggcgat
     4801 gacgcgccgc cctcgcaggt cgaggtggtg caaggcaagc cgtgtctgcg agcctccgaa
     4861 cgcgcgtacg gcgacggcga aatcgcgtag tcgatggggc gtgcgtatca atcgatagtg
     4921 actgtagcgc atccatatcg tggtgaggag tctctcatgc cgaactgtaa gcgcttacct
     4981 taagtagttt gtaatatggt ttacgtcata aaaatagata ttctaaatca tagtgttttg
     5041 caagggcttt tgcggaaaat ggctatttat caacagtttt cgaatagtcc gcatgacggg
     5101 cgactgtgag cacgctttat ggtaaccgct ttccgtagca ttaacgttag agtatgactt
     5161 ttgaacaatc ggcaactctg aagactcagc cccagacctc gggcaccatc tcagcggcag
     5221 acgccgtgat gcaatcggcc cgcgacgaac tcatccccgg caaactcatc tgtgcgatca
     5281 tggccgtggt cattgttatc gtgttcgtcg ccatgctcca cgccctacgt aaccggaagt
     5341 aggttgtagg cgggattggt ctgactggta tccgccacag ccgggttcgg tagagcggct
     5401 accaatcggc cagtgcctct atggcatcga gtcctgttct gcgatgttgt tcgtca
//
LOCUS       DAWBMW010000102         5375 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_3351_length_5375_cov_2.785526, whole genome shotgun sequence.
ACCESSION   DAWBMW010000102 DAWBMW010000000
VERSION     DAWBMW010000102.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 5375)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5375)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5375
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3351_length_5375_cov_2.785526"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3988..3997
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1094 a         1609 c         1672 g          990 t
ORIGIN      
        1 gagcgtctgt ctccggaaca gaaggtcgtg ggttcgatcc ccatcaagcc caccagcatg
       61 gctttcggct aaccgccgga agccttttgc atattcggcg cgtgcaaggc tggtgctggt
      121 gtcggcaatg gcaaggccgg tgatgccggc tcgcttgggt cgtggaaaaa ccgtccggtt
      181 tggcgagaac ggtagccgaa tcgtctggaa tcgtctggaa tcgtctggaa tagcaaggaa
      241 tagtctgttg atggccgtat gccgggcatc atgcgcgcta aacgcgactt tgctcagtag
      301 actgctacgt aatcggcatt gccaatcgtt gctgatcgtg cgattggtcg tagccggtgg
      361 cgatgtgtac cggcgccggc cgcagcaggt gggcgccccc aagatgagaa ggagcacaat
      421 atgagcgaaa agatggaagc cgtagccact tccgaggcgc cggccgcgct gggcgcatac
      481 agccaggcgg tgaaggccaa tggcttcgtg ttcgtatccg gccagctggg cattgatccg
      541 gccaccggcg aactggcggg cgagaccgct ggagagcagg ctgcacaggc gctgaagaat
      601 attaagcaca ttctcgacac tgccggtacc ggcattgagc atgtgtgccg cgccaccatc
      661 tacctgaaga acgtcgagga cttcaaggaa gtggacgcac gttacgctga ggtgttcatc
      721 ggctccgtca agcccgcccg cgtggccttt ggcaacaaca tgattcccaa gggcgcgctc
      781 gtggagattg acgccatcgc ggtggagtga ttcgaacgtt ttcccggcgg cccgtctgcg
      841 cgcatggtgt gtgcagacgg gccgccgcgt attgtgcgac gcattcccgg gaatgttgcg
      901 ctggaatcgt tgggcggtgg tccgtgccac aatagagacc atggcatcca aaggaaaacc
      961 gtcgccgcgt tcggccgtaa gcccgctgag tgatgagcag gtcaagcgtc tggccgcccg
     1021 ccacacgctt gtggactcga cacagtatta cccgaccggc ccccgcacgc ccaatcagcc
     1081 ggaaatcaac gcccagaatc ccaaggccac caaacgactg ctgggagggg tgtccattgt
     1141 gttccgcgcc agctgcaaga ccaaggcctg gggcttggaa caggtgccgg aaaccggacc
     1201 gttcatcacc gccgccagtc atgtcaccat gtttgatgtg ttcgtgccaa tgatgagcct
     1261 gttccacatg ggccgccgcc cccgttacat ggccaaggcc gagatggcga agtggccatt
     1321 gatcggcaag tggttccagt tggtcggcat gcagccggtg cagcgtcatt cgggcaaggc
     1381 caagcagatc gaagagacct cgattgatat tctgacgtcc ggccgcccgc tgacggtctg
     1441 gccggaaggc accgtgaccc gcgacccgaa aaagtgggtg atgagcatta aggagggcgt
     1501 gggatatatc gcccttgaat cctcgcgcag actgggacat caggtgccgc tgtacccggc
     1561 ggtgacgtgg ggtgccgcct ccatcaacca ctggtggcca tggccgcgca aaaacgtggt
     1621 gatgtgctat gaccatgcgc tcgattactc cgatctgctt gccgacatgg actcatgggg
     1681 tgaagagccg cctgccgagg ccgtgaacga gctgatgtgg cgcgtgctca aacgcatgaa
     1741 cacagtactg gaggaaattc gtggcgagca gttccccgct gaaggctact gggactaccg
     1801 cagtatgagt cgtaagcctt ggcctgaagc cggcggcccg ttcccggctg agaagtagcc
     1861 gcaccggttc ccgtacgggg agcctaggcg agtagcatgg cacaagattg tgccgatgaa
     1921 gttgcgcgac aagcgaaggt aggaatttga tatgggtaaa aatataacgg ttctcggtgc
     1981 aggagcatgg ggtactgctt tcggccaagt gctggcggac gcaggcaaca cggtgaccat
     2041 gtgggccaag gagcagcaga ttgtcgaggg cattcgcgat caccaccaca acgccgtgcg
     2101 cctgccgtcc gtcgaaaagt tgccggacaa catgaccgcc actggggatc gcgccgaagc
     2161 cgtcaagaac gcggacatcg tagtcgtggc catcgccgcg cagttcgcgc gagtggcact
     2221 cgtcgaattc aagggactga ttcccgatca cgccatcgtc gtcagcctga tgaagggcat
     2281 cgagcgtggc accaacaagc gtatggacga agtcgtccgc gaatcccttg acttgccggc
     2341 cgaccggttc gccgccattt ccggaccgaa cctgtccaag gaaatcgccg atcgtcatcc
     2401 cgccgccacc gtggtggctt gcacgaacct cgataatgcc acgaaggtgg ccgaggcatg
     2461 caccacctcc tatttcaagc cgttcgtgac caccgacgtc atcggcctgg aaatgtgcgg
     2521 cagcctgaag aacgtgaccg cgctggccgt gggcatggcc cgtggtgccg gctacggtga
     2581 gaacaccgca gccatgatcg aaactcgtgg actggccgaa ctgaccgcgc tgggcgtagc
     2641 cgctggcgcc gatcccaaga ccttcttcgg cttggccggg gtgggcgatc tcatcgccac
     2701 ctgtggctct tcgctgtctc gtaactatac gttcggcgcg aatctcggca agggattgac
     2761 ggtcgaagag gccaccaagg tcagcaacgg tgtggccgaa ggcgtgccca ccacggatgc
     2821 ggtggtggct cttggcgatc agcttgatgt gccgaccccg ttggcgtatc agatgagccg
     2881 cgtgctgaac gaaggcattt cctgctcgga gatgcttgcc ggactgttcg gtcacgaggt
     2941 taccggcgaa taacgagaga agcgacgtat acgtggcgta tacgttgtac gtgcaaagtc
     3001 gcgtggcgta tacgtcgttg acgtgcacga gtagactggt gtgcgtctct tgtgcgcggc
     3061 gttccgagcg cggttcggtc cggttggcgg tgcaaagcat aggcttgaaa acggttgtag
     3121 tttcaacaaa aggatggtta tggccaagaa gcgtgttgtg gtgctctatg gtgggcgtgc
     3181 agacgagcat tcgatttcct gcatctccac tgccggtgtg ctgggggcca tggacactga
     3241 acggttcgag ccgattccgg tcggcatcac caaggacggc aagtggatca tcaatggcga
     3301 agatccgcgt ggctggaact tggacggcgg cgagctgccg accgtaaaga tcaccccgga
     3361 atcgcgcccg gtgatgcttg acccctctcg cggacaggac ggtttcttca tcggcgagcc
     3421 cagccacatc aattccgccg attccggctt cggcaccagc ttcgtcagca tgtccgaccc
     3481 ggaaatgcac catgtgctga cctcccttgg ccatgtggac gcggtactgc cggtgctgca
     3541 cggcccctat ggcgaggatg gtacggttca aggcctgctg gaaatgatgg gtgtgccgta
     3601 cgtggggtgc ggagtgtttg cttccgccgc ctgcatggat aagcattaca ccaaggtggt
     3661 actggatgcc gccggcattc ccaccgcgcc cggtgtgacg gtcgatgcgc gcaacttcac
     3721 cgccgccgat gtgcttgccg agatcgagga cgccggtctt acttatccgc tgtttgtcaa
     3781 gccttcgcgc gcaggctcca gcttcggtgt gaccaaagtc gagaaagccg atgaccgcga
     3841 gacccagcag gatcgtctgg ccgccgccat cgccaccgcc ggcgagcacg actggaaggt
     3901 gctcgtcgaa caggggattg atggtcgcga aatcgaatgt gccgtgctgt gcccgaaggc
     3961 cggtgacgag cccgaggcca gctggccnnn nnnnnnnacg accagttcta cgatttcgac
     4021 tccaagtaca tggatgcctc cgccagccac gtggaagtgc ctgccaatct gccggtcagc
     4081 gtgcttgagg acgtgcgtga cgtggcccgt cgcgcgttca aggccgtgga tggtgccggc
     4141 ttgagccgtg tggacacctt cgtgacgcct gatggcacgg tgatggtcaa cgaaatcaac
     4201 accatgcccg gcttcacgcc gatttccatg tatcccaagg cttgggatgc caccggagtg
     4261 agctacaccg aactcatcac gcgattgatt gagggcgtgc tgaggtaagt gtggtgcgga
     4321 atcggccgca ggccggttcc gctattgaca catcgcccat acctgtcagt aattcatgcc
     4381 aagttttcct aagttcaaca ggcgtacagc aaatggcgaa cgcaattcac ttgcgcgcca
     4441 gtgtcaggac agattaggcg catagtttct tttcttggat tcacgggccg cgcgaatgcc
     4501 gcgcatacca agcccgactc cccagactgt cgtctggatg atgttgagaa aaggaacatt
     4561 atgaacaacc tgcgtaagat cgcggccggc tccctcgccg ccgtgctcgc gttctcgatg
     4621 gccgcctgcg gctcgagcaa cgcctcctcc gatggcaccg gcgaaagcac cggcaaggcc
     4681 gtcaccgtga acgaaaagtc cgtcaaagcc acctccctcg ccgacttcgg caccatggaa
     4741 gacctcgaga aggccgccaa ggaagagggc gcgctgaacg tcatcgccct tccgcacgac
     4801 tggtccaact acggcgaggt catcgagagc ttcaagaaga agtacccgga gatcaaggtc
     4861 accgagctca acccgaacgc ctcatccaag gaagagctcg acgccgccaa gaccaacaag
     4921 ggcaccgacg ccgcccccga cgtcttcgat gtcggccagg ccatcgccgc cacctccacc
     4981 gactacttcg ccccgtacaa ggtgcaggcc tgggacaaga tcccggacac cgccaaggac
     5041 gccgacggcg cctactacgc cgactacacc ggcatcatgt ccgtgggctg gaacgccgac
     5101 aagtacggcg acatcagctc cctggacgac ctgctcgacc cgaagttcgc cggcaccgtg
     5161 gccctcaacg gcaagcccgc cgaggctggc gccgccttca acggctacct gatggccaac
     5221 cagctcgccg gtggcgacat caacaacctg cagcccggcc ttgactactt caagaagctc
     5281 aaggaagccg gcaacctgac caccgtcgac gtgaccaacg gcaccatcga ctccggccag
     5341 accggcgtgg tcttcgactg gacctacaac caggc
//
LOCUS       DAWBMW010000103         5370 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_3354_length_5370_cov_2.686924, whole genome shotgun sequence.
ACCESSION   DAWBMW010000103 DAWBMW010000000
VERSION     DAWBMW010000103.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 5370)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5370)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5370
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3354_length_5370_cov_2.686924"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4965..4974
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1088 a         1559 c         1629 g         1084 t
ORIGIN      
        1 gagcatggtg cccaaacgga agttcttgcc gacgtcacgc aggaagtcga tgacggtcat
       61 cgaggcggtc caatcgtaat tggacacgaa acgtacggcg ttcgcgcctt cggtgtccag
      121 aatgccgccg atctgattct tcaggcgatc ggcccagccg gccaccacgt ccttcgggtt
      181 aagcgtacgt tcgccggact ggcgcgggtc gccgatgagt ccggtggcgc caccgaccaa
      241 agcgatcgga tgatggcccg caagctgcag gtgacgcatg ttgatgagct gcacgaggtt
      301 gccgatatgc aacgatgcag cggtgggatc gaagccacaa taataggtga tgggctcgcc
      361 gttcagtgct tctgcgagtc gatcccggtc cgtggactgg gaaatcaacc cgcgccattc
      421 cagttcctca agcagggtgt tgaaacccgc ttccttgaag tcggtgacgt gagccatagc
      481 ttgtcgcttc tccctatcga attgtgaata agactgccca attgggcaca gtcaagtttc
      541 gcagaacatg gcgacagtgc cgaacaccag ccgagccgcg cgattcagta aaacgcctta
      601 ttctagcggc gcgggcccat aagttcgcgc aaagagtcga tgatgtcctg acgatccggc
      661 acctggaact tcgggaagga cttgagcata cgcttctgct gtgcgttgat gacgccattg
      721 acggcccagc gcagacgctc aaccgtatcc tgcgaaagct cacgcatcct gtcggatacc
      781 gcactggagc cccactgcca cgtgccaccg gcgcgtgccg ccacatcctg caccacctga
      841 gagacggaat cgagcacccg ctcgaattcg agcatgcccg caaccgtgac cgccaagtcg
      901 atgatggtga tcagggccag taccgtgacc agcgtatgca gcatcggcag cggaatcata
      961 tccgtgaccc gctcgacata tggctgcgaa ccaagtacca cgcccacgcc gccgaggccg
     1021 aaaatcaacg cgcccagcag gcagacgcgg ccttggaggt tgaagcggaa gttcgagtaa
     1081 tcccaccagc gggcgtggaa cagctgctcc atgacccacg aggttgtgta ttcgagtatc
     1141 gtggcgccca ccatcgaaat caggaagatg atgatcgggt tatgtacgtt gccgagaatg
     1201 gcgacgccga gtatcgcgcc ggtgccgtag atcgggcaca gcgggccgtt gaggaaccct
     1261 cggttgacca agcggtgctg ttggcaggag acgagaatgg actcgtacat ccagccgacg
     1321 aaactgtaga acagaaacca gaggaaataa tgttcgagtg tgatgatcat cgacgggctt
     1381 cgatctcggc gtaaggcttc gcggcgggca acgcgcgctt gttgaaccgg tcgaacgaga
     1441 aggtctcggc ggtggcggag catttgtccg ccaaggtgac cagatagcct tcgatatagc
     1501 gcggcggcac cggggtcatc gggaacatgt ggttctcgat gctgttgcgt tcgatatcgt
     1561 tgagcttgaa gtccttcagg gcattgacca gggcggcgtg gccatgggtg aagccatgcc
     1621 actggtgttc gccgttatcc cagtcgtgcc agtcgtacag gaagtaatcg tgcaggaggg
     1681 ccgcgcgaat cagggatttg aggtcgacgc ggtaccacag gtgtgcgcgg tcggccatcc
     1741 acacagccag gcaggcgaca cgcacggaat gttcgaacgt ggtgacattg ccgtgctggt
     1801 agcagcagcg ttcgatctgc atgtggcgat gctcgatgac ctcacggccg tgcttggcga
     1861 ccaggcgttg aatctgcgtt cgggtgagca gatgtcccac gattttcggt ccttcccgtt
     1921 ttgggttggt tcagtgcaat cccctgttgt gaattacgat tacccggcga tattcaagaa
     1981 tccatcgagt aataccgtga ccgattgtag ggcacaaata tgtccgtgca cactcatcca
     2041 aaagaacgac aacgggaacc gttaatcgtg gtgggtttca agatagtcga gataaccttg
     2101 aaaaccgcca tcgaggcttg ctacatcgta gccgcgatcg gcaaggattt cagctacttc
     2161 ctcgctccac catccttgag cacaatagac tacgacagtt cgatctgtgg gcacggtttt
     2221 gagcacatgg gccagagggt cgagtggtgc attgatggca ccttcgaccg ggtgggcggc
     2281 gaagtcggct ggttccctca agtcgagcag cgtgaccgta ttcgggtcga gcttggcaaa
     2341 ttccgccggc gtgatgcggc ggtacgatgc ttcactgctc tcatcgtcat cgaccgcatt
     2401 atgcaaagca ataggcatgt tcttcccctc tcgttctgat cttggcaccc ctcaaggagg
     2461 ggcaccacat agctctaaat cgcgcaggcc ggctgaatat agttggccgg atcaatagca
     2521 gtaatggtcg gctgctcacc gcacaccggg caatccggca catgctttgg cagtgggacc
     2581 cgtcgaatat tcatggtcag ggcatccaca gtgagcatgc gagccacgag cggctccccc
     2641 acgccggcaa tcagcttgac ggcctcagtc gcctccagac tgccgatcac gccgaccacc
     2701 gcgcccagta cgccggcttc cttgcaggtc ggaatctcac cggcggcggg caggtcgcgg
     2761 aagatgcagc ggtagcaggg gccttcgccg ggaatgacgg tcatcacctg accggcgaat
     2821 ccgaccacgc cggcatggat atacggcttg ccggccagca cgcaggcgtc attgatgagg
     2881 aattttgcgg cgaagttgtc ggtggcatcg atcaccagat cgtacggttc gatgaggccg
     2941 gcgatgttgc tcgcgtcgac gagctcgtag taggtatcaa ccgtcacatc cgggttgagc
     3001 gcgcggatgg ctgtggccgc ggattccact ttgggcgctc cgacacgtgc agtggtatgg
     3061 atgatttggc gttgcagatt gctcatatcc accacatcgc cgtccaccag tccgatatgg
     3121 ccgacgccgg ccgccgccag atacagggcg gccggagaac caagaccgcc cgctccgatg
     3181 atgagcacgc tggaggccag caaccgcttt tgccctttga cccccacacc cttcaaaatc
     3241 agatggcgcg cgtaccgctc cacctgttca tcagtcaatg ccatgttttc tccatgtttc
     3301 catctcccct cagtccgcta cgcggccagc tcccctcaga gaggggagct aataaaagtg
     3361 tcagtgcaaa taccccgtag tcgggctgga cggcacagcc tgtccctcgg tcactttgcc
     3421 aaggccagac aaatacgccg cacgaccagc atcgatggcg tgcttgaacg cctcagccat
     3481 cagcgggata ttgccagcag aggcaacggc cgtgtacgcc atcaccgcat ccgcacccat
     3541 ctccatcgca tgcctggctc ggacggccga taccagcgtc gatgatgacc ggcacatgcg
     3601 cgttggcgat gatgatttcg atgaagtcac gcatgcgcag gcccatattg gagccgatca
     3661 acgaaccgag cggcataacg gcagcggcac cggcctcctc cagctgacgg gccgcaatcg
     3721 ggtccgggaa catgtagggc atgaccacga agccttcctt ggccagcatt tcggtggctc
     3781 ggatggtttc ggcgttgtcc ggcaagaggt acttggtctc gtgttcgatt tcgaccttga
     3841 cgaagtcggt gtggcacact tcgcgtgcca gtcgggcgat acgcacggct tcctcggcgt
     3901 tgcgcgcacc ggaggtgttg ggcagcagcg taatgccttc gggaatgtag tcgagcacgc
     3961 tgttctcggt ggtctgcgcg cggcgcagtg ccatggtgac gatttgcgtg cctgcatgtt
     4021 cgaccgtagc cttgatgagg ttcaggtcgt agcggccgga gcccagaatg aatcgcgact
     4081 ggaactcgtg accgcccagg ttcagcggct tgtcttccac cgggagaatc ggcgcggacc
     4141 ggattggtga cggcgggctg cggcggcagc ggagtcgcct caacagatgc ttcagtaacc
     4201 ggattaatat tgctcatagc ttttcctttc atccaccctg tacaaactgc acaatttcca
     4261 tgacgcactg gtcgtcgagc atggcgtcgg cacgcttatc gcgcgggata atctcaccat
     4321 tgagttcgat ggccacacgc tcggcgcgga atccgtgcgc ttcgagataa tcggcgacgc
     4381 tgaccggcgt ctcgagcgtg acctgctctc cgttgacctg cataatatcc tttcagatag
     4441 taaaaaaggg tgcacgaaac gaaccgtacc ttaagtggtg cgcccattca tgaacccaat
     4501 catgcgaccg ttcgaccgct gtgatgtatt ggctccatca cgaccgtctc acggctgaat
     4561 tatgcaaacg gaatgaaagc tgtatcggag aacccggcaa atctgcgaca acgattcctt
     4621 acggcggcat gacccgcgtc aagttccccg gtcgaagtct ggcgacttcc tctcagcctc
     4681 acggctcccg ttcgttacgg tttctcagtg tatgccgttg cagtgacaac aacaaaatag
     4741 agctccctca tcagagggag cttcattggg actagaacgt gccgggggct ttgtaggcgg
     4801 agccatccga cgtggatgcg gggaagagct tgagttcttc gacctcggcc ttggcgtcgg
     4861 cgatctgctc gcgcacgcga ccgtaagcgg tgccgccctt gccgttgcgg gagtcgaccg
     4921 aaccgtggct ggagagcact tcgcgtactc ccggagcctt gtcgnnnnnn nnnngttctt
     4981 gacgagccat tcggcgatgt cggtggccag tgcgaagccg gtcggagctt cctcttccag
     5041 gcggtcgccg tcgaagtgca tggtcctgac catgccggtg aaggcgggca gcagcacttc
     5101 gagcgtatcg acctggtcga acacggcctc cttgtcttcc tgcagatcgc gagcgtaggc
     5161 ggtgggcagg cccttcaggg tggccagcag gccggtcaga tcgccgatga gtcggccgga
     5221 cttgccgcgg gcgagctcgg cgatatccgg gttcttcttc tgcggcatga tggaggagcc
     5281 ggtggaatag ccgtcgtcga gcttgacgaa ggcgaactcc tgggtgttcc agatgatgat
     5341 ctcctcggac aggcgggaga tgtccacgcc
//
LOCUS       DAWBMW010000104         5348 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_3369_length_5348_cov_2.752503, whole genome shotgun sequence.
ACCESSION   DAWBMW010000104 DAWBMW010000000
VERSION     DAWBMW010000104.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 5348)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5348)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5348
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3369_length_5348_cov_2.752503"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1006..1015
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1010 a         1561 c         1642 g         1125 t
ORIGIN      
        1 cttgatgaac atggttttcc gcacaaagaa gtaccggaag ctcatgccga ggttcaccca
       61 ctggttcaac ccctgctcgt gcggcaattc gatgccaagt cccaccgatt tgccgccggc
      121 cagtgccgct cccctattcg cggcttccat gatgccggga ccaccgccgg tgatgacggc
      181 gatgttgcgc ttggctatct ggctacccat gcgccgtgct gccttgtaga tcgattcggt
      241 gcgctgggtg cgtgcggagc cgaaaataga gaccgcaggt ccgagctcgg cgagcgcccc
      301 gaaaccgtcc acgaattccg attggatgcg cagcacacgc catgggtcca tgtgcagcca
      361 gtcggtgtcc tgatcggatt tcagcaggtt ggcggtggtg ttgtccgagg gaatcatctg
      421 gccgcgtaga atcaccgggc cgcgatgata ggtgtcgccc agcggcgagg cgtcctccag
      481 atcgaccggg ttcaccggtg tggccacagc ctcagttgcc gtctcagcct ccaacgccgc
      541 cgcatcattc gcggctgtgg cttccaatgg ttccatatcg tccacgactg cggacttgga
      601 tgcggcctcg gctagattct ttgcttcagc ggcctttttg ccgtcgttct tcttggattt
      661 cttctgcttc tttgccatcc aatgtcctcc tgtacggcgc tatcgactct tcgcattcta
      721 gcgcctgaag ctctgcgtac gttttccgcg aaatcatcct tgacttcgtg aagaacgtac
      781 ggtgagtgac actgagcgta cgttttccgc gaaataaggc ctcatctcgt gaagaacgta
      841 cactgagcgc gtctcagcgt acggttttca cgaaacaagg ccaacaatgg agtagggacc
      901 atctaagcca tcagtggcgg aagtcgattt ccacactggc ggtgccgtcg gcgagggtgc
      961 cggggtgttc cgtgggcgtg gtgggctctg tggtgtggct tggctnnnnn nnnnnctgcc
     1021 cgatgccatc accatcaatc gattcgtcat cctccgcatc agccgctgct gcggctgcgg
     1081 tcttctcgaa tcgcttggac tgacggctca gcagcacacc ggaaatcgcg gcggcgatca
     1141 gggaggccac cagcacgccg aagcgcgcct cagcggaaag ttccgcgttc ttgtacgcca
     1201 gcgaagcgat gaggaaggac acagtgaagc caatgccgca ggcgacggca gcgggaatca
     1261 tgtcgcgcac acgcagcccc ttggccatct tgagcccgcc cacatgcgtg gacagccatg
     1321 cggtggtgat gatacccaga ggtttgccga ccaccaaagc cacaatcagt gcgatgacca
     1381 gcggcgaggc caacaatagc gggctcatcg actcgaagtg tacgccggtg gcgaacaagg
     1441 cgaagatggg cagggccagc agtgcggaga atggctggag cttgttggca tagcgttcgg
     1501 cacgcggcga gagttcgccg tgcatttcac gagagggggt cagcaggcca accagcacgc
     1561 cggccagtgt ggggtgcacg cctgcctcga acatcatgat ccaagcgagg ataccgacga
     1621 tgccaaccgc gatccacggc acttttttca gtcgcacgag gtatgcccag atggcggcgc
     1681 atacggcgat gccgatgaac caataccagg cgttaatcga ggagaagaat acggcgatca
     1741 gaataatggc cagcaggtcg tcgacggtgg caagcgtcat caggaacgcg cggatcgaac
     1801 cgggcagggc cttggcgaac agggcgagca cggccagcga gaacgcgata tccgtggcgg
     1861 tgggcactgc ccagccgtgc gacatctccg agaacgggat gctgctgccg gtggtggtca
     1921 gaatcagcgt gccgggttca ccaggcccga tctgagagaa caatgccgtg acggccagga
     1981 acaggatggg cggggcgatc atgccgccca ccgcgcacag catcggcacg gcggccgctt
     2041 tcggattggc aagcgagccg gtggtgagtt cctgcttcag ttcgaggccc acggtcagga
     2101 agaagatggt aagcagacca tcctgggccc agtggccaat aggcaggtcg agattggtgt
     2161 aggggatgaa cagatgcgtt tccgctacgg tctcgaacgc atgcgcggtg gcgggcagat
     2221 tcgcgagcac caggccggtg agcgcgaagc caagcatgat gagtccggaa atacggtctg
     2281 aggcggcgat tcgcctgatg gtggaccaaa gtcccttctt cgcgccggcg gtcgtggcca
     2341 tcgcgtctcc ttgctgcact ctcttggatg aaccgtatgt aatagtacgg aatatctcag
     2401 gacgttccgc tccgggctga gtcgcgaagg gcaaccttag cccaaccctg tgtggccgac
     2461 tcaaccgact caaacgggcc aatacaattg gaaaagcctc ggaagcattt cgctttcgag
     2521 gcttactgtg cgccagacag gattcgaacc tgcgacctgc tgatccgtag tcagctgctc
     2581 taatccgctg ggctactggc gcataccact gttgtccccg tggttcggga gtgcgccaga
     2641 caggattcga acctgcgacc tgctgatccg tagtcagctg ctctaatccg ctgggctact
     2701 ggcgcatgtt gtctttcaga caactcgata tataatacac gccgttcagc tgaatgcaac
     2761 tcggcgtgtt gcggcgagtc gcaccatccc gaccactacc gcaacggcgt atttactggc
     2821 ctcacgcctc gtgtgtacgg actcggtcaa gcaaaaccga ctgcgcaact ttgagacgtc
     2881 cctgcggcac atccacggct tcgcgcagga gtttgggcgt gctggaaccc tctacggacg
     2941 ccttgtccac aatcacctgc ctcacatcat ccaaactcgg caactggaac atcgtgtcct
     3001 gcaacgtacg ttcgataatc gaacgcaaac cgcgtgcgcc ggtcccctgc ttgatagcga
     3061 tatcagcaat cgcccgaatg gcctgttctg tgaattgcag atcgacgccg tcgacggcga
     3121 acagtttacg gtactgcttg atcagcgcgt tggctggctg ggtgagaatc gccgtcagat
     3181 cgtctacggt aagttccttc agcacactgg tgaccggcaa acgtccgatg aactcaggca
     3241 acagtccgaa ctcggccaga tcatcggcat tgacctgttc gagcaattcc tcgtctttca
     3301 tgtcggcgtc gtgccaattc gcgccgaagc cggtctctcg acggcctaat cgcttgcgaa
     3361 caatgtccgt cagccccacg aacgcaccgc cgcagatgaa taggatgccg cgtgtgtcca
     3421 tctgcgcaac gtcctgctcc ttatgcttgc gcgtgccttc caacggcacg gaggcgatgg
     3481 ttccttccaa gatcttcagc aacgcctgct gaacgccctc accggacacg tcacgggtga
     3541 tggaggtgtt ctcaccggat ttgcgggcga tcttatcgat ttcgtcaatg tagatgatgc
     3601 cgtgctgggc tcggcttacg tcgccatccg cagcttggag gagacgttgg agcacggttt
     3661 ccacatcgtc gcctacatag ccggcttcgg tcagggtggt ggcgtcggtg atgacgaacg
     3721 gcacgttcat tacgcgggcc aacgcctgcg caagataggt tttgcccacg ccggtggggc
     3781 cgagcaacag aatgtttgac ttggcgacct cgacgtcagc caaagggtca ttgctgcggc
     3841 gggcagcacg cgtctgggtg ggaacggatt gcttggcttg tttgccggtc tggccactgt
     3901 gcccgttatt cccgtcaagc tgctcagcgg actcctgaag ctccatgttg acgcgcttgt
     3961 aatggttgta tacggctacg gataatgcgc gcttggcgtt ctcctgaccg atgacgtagc
     4021 ggttgagata atcgaagatc tgagcgggct tgggcaggct cagcgaattg acctcggcat
     4081 ctttgacacg ctcctcggag atgatgtcca cgcacagggc gatgcattca tcgcagatgg
     4141 aggcattggg accggccacc agttttcgta cctgatgttc ggttttgccg cagaacgtgc
     4201 aacggggtac gtcctcgttg tagctcacca cacgtcccat gattctcctt tgccgactgc
     4261 gccgcaccgt cgaaccgcca cgctgcgcat tatcattgtc atccttgaaa tacaataccc
     4321 ccggtcgttg ccgaccgagg gtattgcaat gtccggaagt tttactggcg gtgttccaac
     4381 acctcgtcca cgatgccgta ttccttggct tcaggagcgg tcaggaaagt gtccacttcg
     4441 atgtcgcggc gaatcttctc gacgtcctgg ccggtgtgct tggcgagggt ctcctccagc
     4501 cattcacgca tacgcagcat ttccttggcc tgaatctcga tctccgtggc cttgccgaag
     4561 ccttggtcga tggccggctg gtggatgagc acacgggcgt tcggcagcat caggcgcttg
     4621 cccttggtgc cggaggccag cagaatggcg gcggcggagg cggcctggcc gaggcacacg
     4681 gtctgcacgt cgggcttgat gtactgcatg gtgtcgtaga tggcggtcat ggccgtcatc
     4741 gaaccgccgg gtgagttgat gtacatcatc acgtcacggt tcggatcttg ggattcgagg
     4801 accagcagct gggccatcac gtcgtcggcg gaggcgtcgt ccacctgcac gccgaggaag
     4861 atgatgcgat cctcgaacag gcgggagtag gcgtcctggg tcttcatgcc atacggggtc
     4921 ttctccacga actgcggcat gatgtaacgg ttggtcggcg cgggctgggc tgcgcgggcg
     4981 gaggccggca tgaaaccggc tacgccctga cggccggcca agcggcgaac tttgcttctt
     5041 cacttgccat gagtctcact ccccctgctg gttgttgccg atggtgtccg gcgtggtcac
     5101 gagcttgtcc acgaagccat agtcgagggc ttcctgggcg gtgaaccagt ggtcgtattc
     5161 gttgtcgcgg tagatttcct cgagggtgtg gccggtctgc tcagcggtca gctcgctcat
     5221 ggtcttcttc atgtccatga tgagctcggc gttgatgcgc acgtcagtgg cggtgccgcc
     5281 gatgccgccg gaaggctggt gcatgagcac acgggcgtgc gaggtcaggt aacgcttgcc
     5341 cttggtac
//
LOCUS       DAWBMW010000105         5303 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_3410_length_5303_cov_2.787538, whole genome shotgun sequence.
ACCESSION   DAWBMW010000105 DAWBMW010000000
VERSION     DAWBMW010000105.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 5303)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5303)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5303
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3410_length_5303_cov_2.787538"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4249..4258
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          981 a         1581 c         1728 g         1003 t
ORIGIN      
        1 caacaactac atcattgatg ccctggctga ggtcgagtga ccatcggtct gaccgattga
       61 attcgctcca tcgggcgtgt acacatcaat tatgatgcca gtgcgcccga tgggctgatt
      121 cgatagtttc gccatgtgcg gtgttgtgtt atgcccgcat ggcacctttc cattccttct
      181 ttctcctttc tctccttccg ctgaaaacgg gactgtctct cgaagtgtgt aactggcctt
      241 aactggccgg tcgtgtggcc ggtcggaaag gctggtggac gatgtctgct gggacaaggg
      301 gaaacatgac accgacgaag aagccggacg cggaaaaacg caacgccgag cgggaggcgg
      361 cgctcacatt cgtgcgcatg gccaaggaaa aaggactgga cctgacaggg cccgacggac
      421 tgctcaaaca gttcaccaaa agcgtgctgg agacggccct ggacgaggag atgaccgagc
      481 acctgggccg cgccaagcac aagaaatcca aggacggcag ggccgcgaac acgcgcaacg
      541 gcaccaccgc caagaccgtg gtcaccgatt cggtaggtcc ggtcgggatc gaggtgccgc
      601 gagaccgcga tggctcgttc gagccggtcg tcgtcaggat acggcagcgg cggctgcccg
      661 gggtggacga ggtggtgctg tccctgtatg ccaggggcct gaccaccggc gagatcagcg
      721 cccacttcca ggagatctac ggggcggacg tgtccaggga gaccgtcagt cgcatcaccg
      781 agcgggtcgt ggccgagaag gacgaatggt gctcgcgtcc cctggatcgt gtgtacgcgg
      841 ccgtgttcat cgacgcgacc gtcgtcaagg tgcgcgacgg gcaggtcgcc aaccgcgcgt
      901 tctacgtggc cgttggcgtg gatctggagg gcggccgcga cgtgctgggc atctgggcgt
      961 cgcccgccgc ggagggcgca cgctattggc tgtcggtgct caccgagctg aagaaccggg
     1021 gcgtggacga tgtgttcttc ctgatctgcg acgggctcaa aggcctgccc gacgcggtgg
     1081 gcgcggtatg gcctctggcc atcgtgcaga cctgcgtggt gcatctgctg cgcaacacgt
     1141 tccgctacgc gtcgaagaag gactgggacg ccatcaaacg cgatgtcaaa cccatctaca
     1201 ccgcgccgag cgccgccgcc gcggcggcgg cccgcgacgc gatgctggac aagtgggagg
     1261 cgagatatcc cgcgatccgc aggctgtgga tggacgcgtg ggaacggttc ataccgttcc
     1321 tggactatga cgtggagatc cggcgcgtga tctgcacgac caacgccatc gaatcgctca
     1381 acgcgagatt caagcgctcc atccgcgccc gcggccactt ccccgacgag caggccgcgc
     1441 tcaagtgcat gtacctgacc gtccgctcgc tggaccccac cggcaaggga cggatacgat
     1501 ggagcgcgcg ctggaagccc gcgctcaacg cctttgccat caccttcgcc gaccgctggc
     1561 caagcgaggg aacacaacaa tgaaaacgcc agttacacac ttaacgagac aggcccggca
     1621 tgagttcaag atggacgcca tcgcggcctg cttcatcggc ggcacggccg tcaccggcgg
     1681 catcggcacc atccccggcg ccatggtcgg cgccctgatc atgggcgtgc tcaaccaggg
     1741 cctgtccatc atgggcgtgg acgccgcctg ggtcaagacc atcaagggcc tcgtcgtcat
     1801 cgccgccgtc gcctacgacc tgctctccaa gaaaaagaaa ggctgagcta aacctggcct
     1861 ccgggcccgc ccggtggcca cggggatgga cctgagttcc attcctcctc cgaaagggac
     1921 gctgttgcac gcagcgtccc ttttccgttt gtcgcgatgg atggatcccg gtgtacgttc
     1981 ttcgcgaaat cagccctgac ttggggtgta tttcgacaat atgattcggc gatgtgcctc
     2041 agcggatatg tgcggccaat gaaaaagcgg gataccgatg tcgatatccc gctttcagcg
     2101 aataggtaag gccagagctt acttcttcct ggctttcttg acgcgcttgg gggtgggagc
     2161 cattttcttg ggttcgaaga acggccactt gtgcaaagcc caggcgtgca gtgcggggat
     2221 cttggcaacg gcgaagccga tgaccagacc gattaccatg gcccaggtct ccagatcatg
     2281 gcctgcgacc agcaagccga tgaacactat ggcaacgatg aagctcacca tctgcgaggc
     2341 ctttgactgc ttggcggtca ccaggtcggc cttcatggga acaagcccca gaaacaggcc
     2401 gcaaggtgtg cagatgacgg ccaatatcgc gctgagggtt attgacaatg tggttgcatc
     2461 catgatgttc ctttagtgct gtgggttgtg gttttctttg gcgtctgcgg tgtcggtgtc
     2521 tgcggtgtcg gcaacgtcgt cgtgttcgtt ttcttcggct tgttcggctt cggcctcggc
     2581 gtccgtgctg tcggtttcgt cgtcatcagc cgcgtcatcc ttgccgggct ggcgccacca
     2641 caggattagc gcgttggccg cgcagtagat ggccgcgatg ccaatgcata ccagaaaaat
     2701 gatgttcgaa gtgctgatga tcgtgccgtc gttcatcgac tctttgacag actgccatat
     2761 ccacatcacg caggcgacgg cggtcagggc gtacagcatc atacgggagc ggtcgttcat
     2821 aggatcatct ccagacttgt tcaaacgact tgattatagc cgtatttgtg gattgcgtgg
     2881 gaaacgaaaa atgccgccat gtgtgcttca gcgaggagcg cggtataaca gcggctcttc
     2941 gcgatgctcg ccatggcggc attcatcagg aatcgttatt cagttgttat ctcatggatt
     3001 tgctggcagc gtatcagccg cgggccttgg cgtacgcctc gtacacggct tcggtgggct
     3061 cgccggtctc cacgccctcg atggtgagct gccaggtcgg cggttcggcc tcgccggaca
     3121 gcacccaggc ggcctgacgg gcggcgccga tggccacgta ttcgtcggtg gccggacggg
     3181 tcacgtccat gccgaggatt gagggggcca gcgtgcggat ggccacggac ttcgcgccgc
     3241 caccaatgag caggatgcgg ttgatctcgg cgcccagcga gcgaatcagc tcgaggcagt
     3301 cgcgctggga acacagcagg ccttcgacga acgcacgggc cagattctcc ttggtggtgt
     3361 tgtgtagggt caggccagtc agcgaggcgg tggcgtccgg acggttcggc gtacgctcgc
     3421 cgtcgaagta cggcaccagg gtgatgccgc cggcacccgg ctcggccttg aacgccagtt
     3481 cggccagttc gtcgtagtcc acgcctaggg cggcgcgacc ggcgtccaga atgcgcgaac
     3541 cgttgattgt gcaggcgagc ggcagatagt ggccggtgca gtcggcaaag ccggaaatgg
     3601 cgccggtcag gtcgtacacc gggttttcag cgatggccgc ggccacgccg gaagtgccga
     3661 gcgatacgga cacatcgccc acggccatgc cgaggcccag cgaggccatg gcattgtctc
     3721 cgccgccggg gccgatgatg cagccgcctt caacgtcctt gccggcaatg gcggggtcgg
     3781 ccttgacggc ggctgcctca cgcgggccca gcacggtggg cagcacaatg gcgtcggcgt
     3841 gggccttggc ggcttcctcg ccctcggcag gggccagcac catggcgatg agatcgcggc
     3901 ggtactcgtc atgcgcggca tcgtagtaaa tggtgccgga agcgtcggaa cggtcggtga
     3961 acagggcttc gagatgagcg tcctcgccct cggccaccgg gccatagccg gcgatacgcc
     4021 agctcagcca atcgtgcggc agacagacgg cggcaatctt cttggcgttc tcaggctcgt
     4081 tctcggccac ccacgccacc ttggtcagcg tgtaggaggc gacgggggag gatccgacgg
     4141 ccttgaccca gcgttgcttg ccgcgggcgg tcacgtcgtc cggttcgtcg ccctcggccg
     4201 gagctgcacc gagcttgtcg atcaaggcgg cggcctgcgg ggcggaacnn nnnnnnnnat
     4261 tccagagcat cgcatcgcga atcacgttgc cctgcttgtc gagaatgacc atgccatgct
     4321 gctggccgcc aaccgcgagg gccgcgacat cgtcaagccc gccggcctgc tcggcggcct
     4381 tggtgaaggc ctcccaccag aattccgggt tgaccgaggt gccatccggg tgcttggcct
     4441 gcccgaaccg aacctgttcg ccggtggcgg cgtccgtgat gcggaccttc gttgattggg
     4501 ttgacgtatc tacgccgaca accagtactc tcgtcatgca ttcctcctcg atgagggtcg
     4561 cgctcaaaag tttcggcgcg ccatagccaa taagttagac gattaaacta tactgtatct
     4621 caacgttcga ttgcaaacgg aaacacgcga aggtgcggtc cgattcaatc gtttgacgca
     4681 caatatcatg gtgtgataga cggatgtgag cgcctgtgcg gcgctcgaga ctaggggtgc
     4741 gaggaggtag gggccaatgg caaatctacg caggatcaat caggaggatc tgcgcaacca
     4801 taacctgtcg gtgacgctcg acaccttatt gcgtgcgcag aaaccgatga gtcgtgccga
     4861 tctggccaag gagacgggcc tgaccaaggc cacgctctcg ctgttggcct cgatgctcat
     4921 tgaaagcggc gtggtgcagg aaggcgagcc ggtcgtttcc accacatatg ggcggcccag
     4981 cacgccgctg gagattcgcg gcggatccat cgccggcatc ggcctgcaga tcaacaccga
     5041 tggttacggt tgcctggcac tcgatctgaa cggcgacact ctcggccggg agtgggtgag
     5101 tgaggatatg accggcactg acccgtacga gatcttcgcc aagctcgatg cgatgacttt
     5161 cccgttggaa agccggctca aacgacgcgg ctgtaaggtg gtgggagccg ggtttgcgct
     5221 gccgggcatc gtcaccgacg acatgtggct gttggtggcc cgcaatctgg gttgggaaaa
     5281 cgtcaacctc acccggttca acg
//
LOCUS       DAWBMW010000106         5298 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_3415_length_5298_cov_2.928285, whole genome shotgun sequence.
ACCESSION   DAWBMW010000106 DAWBMW010000000
VERSION     DAWBMW010000106.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 5298)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5298)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5298
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3415_length_5298_cov_2.928285"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          970 a         1601 c         1657 g         1070 t
ORIGIN      
        1 tctggccgtt ccgggtcttc gtgtacgtga agcccgcggg gtctccattc tcggaggcgc
       61 tgtcggagct cacgacccag ctggacagcc tgtcgtgaat cgtcacgtcg gcatactcag
      121 ccgtcttggt gatctcctga taaatggact cgaagatgtt gttcagctgg ccggcatccg
      181 tggcggcctt gtagtagctg ctggtctcgg cgcggtcacc gaggttggcg ttccaagact
      241 ttccccacaa atgctcactg ctcgttgcag tggcatccgg gtagttgctg gagatgccgt
      301 gcatgtactt gttcaggttg ctcgaggtgt cgccggggtt ggcaccgctg acgacgccga
      361 tcgagtagat ggtcgtcccg gcgtccttca ggctcttcgc cttgttcacc gcggtggcgg
      421 cgacggtggg gtcaaacccg ctgccgtggt tcggctcgcc gtcggtgaag aagatgacga
      481 ccttcttcgc gttggcgcga ggctgatacg ttagcgccgc ctgggcgcgg ttgaaggcgt
      541 agtcggcgga ggtcgcgccg gacgcgctca ggccgttaac cgtgctcgtc agtccggaga
      601 cgtcgtacgt caggttgctg acgatctgcg tgtagttgta ggaggaccag ccttcgcggt
      661 aaaagtcatt gccgacgctg gtcttctcgg tgccggcgaa cttcaccagg gcgatgcggt
      721 tccgctggct ctggtcggta atcttcgcgt tctctgcagc cgtcgcattg atgaatttat
      781 tgaccgcggt cttgagcgag tcaatcttcg taggctggtt ccaaccgcta gcgatctttt
      841 cggccatcga accggacaca tccaacacca gcacgatgtc gagtggctgg ttcgtcacga
      901 tctcgcccgt acccgcgctc ttcgcaccgg tgacgttcaa cgccaccgtg tacgtgccgt
      961 cttcattggc ggtaacggtc ttctcgcggg ccggagcaga caacgtcgga tcatccggca
     1021 cgccgtttgt tccctcggtg gaaagaggag caggcgcgct ggcggccgcc ttctcatcgg
     1081 tggtctgcga ttgtgtcgtg gaggcattcc catcgtccgc catggcggtc gcgctcacac
     1141 cggcgacgcc accgagcatc gccacggcgg caacgatcgc cactaaccgt ttgcctttcc
     1201 tgcccgccgc ggcccctacc gctgcgaacc attctgcaat tctcttcata accttgcctg
     1261 tctcttacga tcacgtacgc caaaacccgg cgttacgctt ataaggcgtt acggtattaa
     1321 gcaaaaagca cgaatgcgta cacccgggta gagggaattt tttacccgac actgaaaaga
     1381 accttggaat ttttgaaaag tggcaccctc gtcatccacc ctgcgataac gcacttttct
     1441 tctcgacggg gcgtgccggt cccaccatct gggaactgag gcggatttgc cgccgctgcg
     1501 cgggggccgg agttactgac gaccgtcagt atgccactgc tccgcatgtg tgcagtatca
     1561 cataccgcta cgcatacacg gagacatact acatgcaatt tctgtgaacg caaacatacg
     1621 actcaccaag gccgtgttct gttgcgggcg gtggtgttac gggcaatgtt gtgggtagta
     1681 ccctagccac aaacctatat agtggagcgg atgacgggaa tcgaacccgc gtaatcagtt
     1741 tggaagactg aggctctacc attgagctac atccgcgtta agcgtcgctt ttggcgactt
     1801 gagttattat acatatttct tcggggatgg caaacgcgtg tggctgcagc aggaccagcc
     1861 aatctagttg atggattgcg gctttgccga ggactcccct cagtcgccta cggcgacagc
     1921 tcccctcaaa gaggggagct ggaaaccgtc gaaatcacaa atggatgagc tgggactcgt
     1981 atggttggag gtcggcgccc atcgcattca gaatcacgaa ccagcagttt tcgggcagcg
     2041 agtcgagctt gtcgatctgg ttcttgagca ggcgcacgtc gcggctggtg agcgcgcccc
     2101 acaggttaac catgatggag acgttgtaca gcatgtagca ctcgggggcc tcgggcgcta
     2161 ttccacagaa atcgtcacca ccgcggaggc aatcgaacca tcgtggctca gggaaatatg
     2221 gatatgcggc atcgaccccg caccgatagt cgagcgctgc gccgaacgcg cattccgccg
     2281 ctcctgcatc actgcataag gcccgttatc ggaagcagag gaataagcag aggaatagcg
     2341 agcgccggca gccgaattcg cgtaatcagt ttggaagact gacgccgccc ccttacccag
     2401 agacacatgc ggcacaccac gggaatcgtc caagatctcg atttcaggcc acgggaaatt
     2461 atcaagcgtg aacggatagg gggcatcgcc aagataatcg caccaggctt tgaggaacgc
     2521 ctccttgccc gcccatttgg cggccagatg gacggcctcc ccgtcattct tctggcgcgc
     2581 gcggtcggat gcctgccgca cctcacgcac ggagaacagc gcacgcatgc gagaccccgg
     2641 ctccgccaac tgttcggcga atgcggccac atccaccacg tcatgcccca aaccacaagt
     2701 attcatgctt cctattgtgt accgcttcgc gcgtaatctc tacacctcat cagaattggg
     2761 gcctgatttc catttcaggg ggaaaagatt cactatgctg gatgacctcg cgcaataccg
     2821 caaacatctt gggctggctg cctagccgtc caaaaaaaca tccgcgcccc gccccgaagg
     2881 tctatctccg tttcgagggg cggattttga ccgttccgga ggtctacttc cgtttcaagg
     2941 ggctggtcat cctccatacc acgagtatcc gcagcataac gccaacgcca ttccgccgtt
     3001 tttacgcctt gtctacttgg gtaacagccc cttgaaacgg aagtagacct ccggcgaatg
     3061 ccaaatcagc cccttgaaac gcagaaagcc cactccgagg gaggggcttt cgtatgttct
     3121 gcatatttct ctcactgagt ttgtgccagc gagaaagctc caggggaaac tcccctcatc
     3181 cgcttcgcgg gagctcccct caaagagggg agccgagatc cggtgcgcta ctggctccca
     3241 tcaaccgagg ggagccagag gaatcaggcc tcgtagtagc cgtcggcacc gaggcgggcg
     3301 tccgggttga ggagcatcgc cttttcgacc tcgtggtggt cgtagccacg gtgatcctcg
     3361 cggaagcgac ggttgtcgat cggctcgtag agcgcggcgc gacccatcat gccttcctca
     3421 aggtgacgct ggccggcagc caggcgggcg ttggcacgct cacgccaagc ttccagtgcg
     3481 gcctcaccat cggccttggc cacggccgcc tcgaaggcac ccggatggac cagagccacg
     3541 aagccggaaa catgtccgaa gccgagcgag gtggccagac cggccttgac cgggcgacca
     3601 gcggtctcgc gaccgaactc gtcctcacca ccgccgatgc gcaggggctt cctaacccag
     3661 accatgtggt cgtcgcgctg gagcttcgga tccacgcagt cgagcgccgc gttcgccgga
     3721 atcacgccgg acttgaacag ctgcgtcaga ccgttgacct ggaagatgca ggcaccaccc
     3781 ttggcgtggc cggtaagcgt cttctgcgag atcacgaaca gcgggttgcc gtcggtgcgg
     3841 ccgatggcgt gggccagcgt gttgtgcagc tcggattcgt tcgggtcgtt ggcgttggtg
     3901 gacgtgtcgt gcttggagac cacggcgatg tcgtcggctg agacgccgag cttagccagg
     3961 tcgtgcacga gcttggagtc cttgccgccg aggccggcag ccagggcacc gaggcccgga
     4021 gccgggatcg aggtgtgagc gccatccgcg taggagtgga tgaagccgac cacggccgcc
     4081 accggcaggc cgagcttttc ggcgatgtca ccgcgggtca cgaggatcgt gccgccgccc
     4141 tgggattcca ggaagccgcc acggcgacga tcgttcgcgc ggctgaagaa gcgcgcgtcg
     4201 atgcccttgg cgtacatttc ctccgaattg gcggtggcgt tcatgttgcc gaagccgatc
     4261 acggattcca caccgatgtc gtcgatcgca ccggtgacca cgaagtcggc cttgccgagg
     4321 gcgatcttgt cgacgccttc ttcaagggag acggccgcgg tggcgcaggc ggagaccggc
     4381 tgaatcatgt tgccgtagcc gccgatgtag gactgcatca cgtgagccgc gaccacgttc
     4441 ggcagggctt cctgcaggat gtcggtcggg atctcgtggt tgaggaagcg gtcgaggtac
     4501 agcttgcgca tcgacatcat gccaccgaag ccggtgccct gggtcgaagc caccagcgag
     4561 gggtggatgg actcgaggat ttcggccggg gtgaagccgg cgcccaggta ggcgtccaca
     4621 gtggtgacga tgttccacag ggcgatcttg tcgacgtcgc cgaccatcga ggcgggaatg
     4681 ccccatcggg tcgggtcgaa gcccttcggg aactggccgc cgactgtgcg ggtcatggtg
     4741 gcgcggcgcg gcacgcggat catcgagccg gcgtggcggg tcacgttcca ttcgccggat
     4801 tcctcatccg gggcgatggt ggtgtgagcc tcgtccaact tgacgtattc ggtggcgact
     4861 tcgcgggtgg gcacagagaa ggtgacgtcg tggtcgagga agacctcggc ttcctcttcg
     4921 tcagcaccgt ccttgtagtc gttgcccatg ccttcctcga acgggcggat gccggagcgg
     4981 gccacgacct cgtcgtggta gcgctcggcg atgtcctctt cgggcacgag gttgccgtcg
     5041 gtgtcgtacc agccgggctt cgggctgtcg gcccaagtca gcaggcccat gttccacgca
     5101 agttcgagca ctgcgccggc gctcaggtcg acggtgccgt ccgagtggat gccgagttcg
     5161 gcctgggcgc gcgtacgacc ggagccccac gggcccagct caccgacgga aacgatgacg
     5221 atttcgtctt ccggcttggc agtgacgttg gtccaatcgt tcaagtcgac cggggcctgc
     5281 ttggtgacga tcggtgtg
//
LOCUS       DAWBMW010000107         5097 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_3580_length_5097_cov_2.199127, whole genome shotgun sequence.
ACCESSION   DAWBMW010000107 DAWBMW010000000
VERSION     DAWBMW010000107.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 5097)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5097)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5097
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3580_length_5097_cov_2.199127"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1392..1401
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2878..2887
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1124 a         1596 c         1396 g          961 t
ORIGIN      
        1 cttcggcgat atcctcgccg gctactatgc caagctgctc ggtctgccgg tcaagcacct
       61 cgtggtggct tccgacaaga acaacgtgct gttcgacttc ctgaccaccg gtacctacaa
      121 ccgccagcgc ccgttcttcc agaccatctc cccctcgatg gacatcctca tctcctccaa
      181 cctggagcgc atgctgtact acttgtccga gggcgacacc cgtctgatct ccatgctgat
      241 gaacgacctc aacaagtggg gcacgtacga gatcccggaa gaactgctgg ccaagattcg
      301 tcagatcttc ggcaccggtt gggccgatga ggatcaggta cgcgagtcca tcaagcactg
      361 ctgggacgag aaccactacg tgatcgatcc gcacaccgcc tgcggctact acctacttga
      421 gcagatgccg cgcgacccgc tgaccccgcg cgtgctgctc tccaccgcca gcccctacaa
      481 gttcccgcgc gtggtgaacg aggcattggg cttcgacgcc accggcaccg acttcgagtg
      541 catggatgtg ctggcccgcg aaaccggtac caccgccccg gccgccctgc gtggtcttga
      601 gaccgccgac gtgcgcttca aggacgtcgt ggccatcgac ggcatggaag attacgtcga
      661 aaaggccgcc cagacgctgt aaacatacca gcaccttggc tcccctcaga gaggggagcc
      721 attttttaca tcagaattgc gtttacatca gtatcgcggc gactggcagt cctatggcga
      781 ttgccaacag cacgatagtg ccgatgatca cgctggggcg tgcgaggttg acggtccagc
      841 cgacgccgaa acgtttagga accatgatgg ccggatcgtc acggttgatg tagatgatgc
      901 cgccaatcca gtgcaggtca tcgtcatacg gcataacatc atcgtccgaa tcgtcaccgc
      961 cgtcactgtg ccgaacaccg gcatagacgc gcgccccgtt ctgcccgtaa tacacgccga
     1021 tcatcagcgc gccgaccaga gccgccacag cgatgaacat gccgatgatg ccgattagat
     1081 tgacatccag cacgccaatg gcgacaagct gaacgcctag gcccaatcca ccggctgtca
     1141 gcagtccgat gaccagcacg tagatgctcc agacccgcga gaacacacca taggcatacc
     1201 cggtggtggc ggggtggtcg ggatcaatcg ggtgcttggc gtagatgatc gcgacatggc
     1261 tggccaccat caccgctgcc agcaccacct gcgcgagcac ggcgtaccaa atcaccatcg
     1321 gactcttctc ggcccagccg ttcacattgc cggcgatatc cgcatgaatc ggcacccgct
     1381 ccggcatccg gnnnnnnnnn ngcccagcag cccgaccgca atggtgacag cgagacaaac
     1441 gacttgcagc aattcccact tcaggctcag cgggtcggga ttgtcccgct cacctatcaa
     1501 tgccgcacgg cgagcactgg acgcctccca tccctgattg gttttgatgg acagtacttt
     1561 gcggcgaaac acctgctgca gcacaaaacc acatatcgca atcaccaccg ctgcgatgga
     1621 cacggtccag aacgccgcgt cgactccaca gagccgccag acggccaacg agaccagcat
     1681 ggcgcacaca ccgattgcca ctacaattcc cgaaaacgcg actttgtatt cgcgaatgcg
     1741 cgtattggta tgggcggcgg ccggcaccgt gacaccgaac acctcatgcc ggtcggtaac
     1801 ccatggaacc cctgccaggc cggcggtcac tatcaccggc aacagcaaga acgcacacaa
     1861 cgcgatgttc atcgacacga ctgtattgtt catcgttatc tcccctgatt ttcttttgtt
     1921 gcaccgtcag taacgctctg cgaggagcgc acgccgtatg cccgttcgca ttgccgtttg
     1981 gcggcatcga ggaatgcggc cgaatcaccg ccgcgcgcct tgtaggccaa ggcaaggcga
     2041 tagagttcct cgtccatccg ctcatcctgt gcttcggcag ttcggttcgg cgcttctccc
     2101 ccgccgttac gctcagcgac ggttgcaccg gcaccacgac gcataacgat gtatccctcg
     2161 tcacgcaaca gcgcatatgc cttgttcacc gtgtgcaggt tgatgccaag gtccacggcc
     2221 agcgagcgca cggacggtaa cgaatcgcct ggcgacagct catcgcccgc gatggctgcg
     2281 atgaactgcc ggtgcagctg ctcgtaaatc ggcacgtcac tgcgctgatc gatttccaca
     2341 atcatggtcg cctcctccca aaccgttcta tttgttctac atcaactata acacttaccg
     2401 aatacaccgc aaggcacacc aaacataaca acatatttaa tatgtctgct atgatggtgc
     2461 ccaagttgcg gcgcactggc gaggaggcaa tcatgtggtc ggaagaggaa atcgatgctt
     2521 tgctgaaccc gctgcgttcc gcgcaatcgg acagtcagga tattgaggtc aaggaggccg
     2581 tgggcggatt gccggtttcg ctgcctgaga ccatttccgc tttcgccaat ggacgcggcg
     2641 gcaccatcgt gctgggcatt gccgaaaagg atggcttcga acccgcccaa gggttcaagg
     2701 cgcaacccgt cgccgaggca ttggcgcaga tgtgcagaga caaaatcacc ccgcctataa
     2761 ggccagtcat cgacgtcgct tcgtatcatg attccaccgt ggtcatcgcc gtcatcgatg
     2821 aggccgatcc actggataag ccatgttacg ttcgcaatag aggccggtac agcggctnnn
     2881 nnnnnnntcc gagtttggga tggcgaccgc aaactcagtc attacgaaat cgaccggctt
     2941 ttggaaaacc gcgcgcaacc catgtatgac cgagggctga tctacgaagc caccgtcgac
     3001 gatctgctac cggatatcgt gcagggcatt cttacgttat gccgcgaacg ccgtccacgt
     3061 gtcttcggaa aactgaccga tgaagaggcg atgagcgcgc ttgggatagt cggaacagga
     3121 gatgacggga agcctcatcc gacgcttgcc ggactgcttg tcgccggcaa ttatccccag
     3181 cagtttctgc cacgggtgaa tatcacgttc acgtgctacc ccggatatga caaggtgttt
     3241 gccggcgggg tgaaattcat cgacaattat tcactcgacg gccccatccc cgagatactc
     3301 ggagaaaccc tgaacgccgc acagaagaac atgcgcatcg gaggcgtatt ggacggccca
     3361 ttccgcaaag acacctttga atacccagag gacgccatac gtgaggccgt ggtcaatgcc
     3421 atcatgcatc gcgactattc gccgatggcc cggggcggcc aaattcaggt caacatgtac
     3481 aaggaccgcc ttgaagtgct gagtccaggc ggattatacg gcgacgtaac gctggattcc
     3541 atcagcgagc ccggctccac ttcgacccgc aatcagacac ttgccaaatt gcttgaatgc
     3601 accccgttcc gcaatgccac agtcgcggag aatcgaggga ccggatacag cctcatcaac
     3661 agactgctgc tggataacgg caatggcgag cctgaaatat acagcacgct gaatttgttc
     3721 tcggtcacct tccgctcagc ggatacctcc aagttgccgg acagccgcag ggatccccgt
     3781 tacgcgggtt ggggcgatag gcccgatatc gattacagcg cggcgcaagg gctgatcaac
     3841 ggacagcttc cgacaccggc gcacccgaaa atcgtgccat tccccaaaaa cagcatcaaa
     3901 acggaagagc tcagcgacga agagatgtac gagcgatatc tcaaagatat tcccggattg
     3961 gggccgacca ttgcgcggat agccggactg gacgttccgc agtccgcgga catgccgctg
     4021 gttcgtgatg tctcatccat cgaaaggcca tcagctcgca gcgcgtcggc atccgcattg
     4081 gaaggcagcg cccgctactc catgctggaa gagcaactgt tgcaactgat caacaagcac
     4141 ggcaccatgt ccacgcccaa attggtggag gccaccggag ccccgcgctc caccgtcacc
     4201 tatcagctgc gcaaactcct ggaatcaggc acgatcgaac gcacgcaacc agcccgctcc
     4261 cccaaacagt cctaccggat aaagtccgcc tgaggcgtgc aaagcgaaaa aaggcgcgtt
     4321 ggcctgtgcc tcggcatatg tccaacggcg tgttgcttgt ttttccagga aggcgggaat
     4381 cgccatgccg cgcggtacgc gagtgatatt acgcgggttt cagtgcgttg tgtcaaccaa
     4441 acgcgagagg aacatatgac cacggccaac gggcagacgc ccgctgaccc gatgcttatc
     4501 atgcttatga tcgctgcggc catcaccctg ggcgtcaaca tcacccgctc catggccggc
     4561 ggccacgccg acattctcga atgcgtgggc attttcttcg ccatcgccct gtccgtgacc
     4621 atcaccgtgg tgatggaagg ccgcagcgcc aaggcattcg aagcgctgaa cgatatcaat
     4681 gacgacacga ccatcaccgt ggtgcgcgat ggcgagttcg gcaaaaatcg tccgcgaact
     4741 gcgcgccgcc gacaccggta tgacaccgct gcaggagaag ctcgcaaagc tggacgaact
     4801 tcatgggcgg caccgccgaa cagcagtcga ccattctgtt cacgctgttc gtggtattcc
     4861 agctgttcaa cgccttcaac agccgcgagc tgggcaacgc cagcctgttc gccaatctgc
     4921 tgcgcaataa ggtgatgatc ggcgtgttcg cgctgatgtt cgcacttcag gtgctggtgg
     4981 tgcaattcgg cggcgcgatg ttccgcaccg tgccgctgcc gatcgacgtg tggctgaaga
     5041 tcatcgcggt cggacaaatg cgcggctaga gttctttggc tcggtagatg cggatgg
//
LOCUS       DAWBMW010000108         5014 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_3645_length_5014_cov_2.327485, whole genome shotgun sequence.
ACCESSION   DAWBMW010000108 DAWBMW010000000
VERSION     DAWBMW010000108.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 5014)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5014)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5014
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3645_length_5014_cov_2.327485"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1290..1299
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1700..1709
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4421..4430
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1132 a         1394 c         1459 g          999 t
ORIGIN      
        1 cccgaaacac taacccatgc acgccccacc gcaaacagcg aataaccaaa tgtatgcatt
       61 ttcggcacca tgagcgaagg agttcgcaaa cacaacagtg attggcatat acggcatcga
      121 ccggatgaac agcatcgcgt cctcataggt caagacccac tgggactcgt cgcccttggg
      181 tatagggaca ttgcctgtgt catagtcgtt aagcagcaca ggactcggca aatatcgttc
      241 caagatctcg gcggtgccag caatttccgg ttcggtggac ctcgatagca ccttgacgac
      301 ggcgtgaagt gcgtcaagcc gcttgcgatt gatggtataa ccatcaacga gatgctgctt
      361 cagaactgag ttggcccatc gccggaaata gatgccttcc ttggatttga cgcggtatcc
      421 gaccgacagg atcatatcca ggttgtaatg ctcgacttgg tatgtcttac cgtcagatgc
      481 agttgtcgca aattttgcga caactgaaaa tccattgagc tcttccctta atgcattgcc
      541 gacatgcttg ccgattgttt tgatatcgcg gccaaacagc aaggccaatt gctgtctgtt
      601 aagccacacc gtgtcctcgt ctaccttcac atcgaggtgc acggtgtggt ccgcgctctc
      661 atacaaagca atctgccgct tcagttcctc gccagtcatg agatcgcctt tcttttttcg
      721 ggcgtctgta aatcatgtcg ttgtcgtcgc taaattatgc atccagttta tcagggcagt
      781 cgaacgtttg ttcgattact gtatctctca tatttgtatg actttcccgg ctcccgccat
      841 caatcaccag tcgcaggtaa tcgcggtcgg cggacttgcg gcgtggcacg aaacgactga
      901 tgatggagtc cttcttgccg cggttcttca cgtacaggaa accgaacacg gagaacacga
      961 ccgcgccgat gaagttgacg accagatcct ccatcgtgtc gatcagaccg atgtcgaggt
     1021 agccgccgag gcccaggtct tggccattga ccgtgacctg ggtaatgtcg ttgatgcgaa
     1081 tcgcatggtt ggtgtgcgtg gggtcgagca tcaccgagct gatggaatgc acgatggcgt
     1141 ccttctgcat gtcgaacccg aacaactggt ccatgccgaa ttcgaagaac tcccagatca
     1201 cgccgatggt catggagaag cagaaggaca cgatcgccag atacaggggc gaaagctcga
     1261 acttcacccg gtcggaccgg ttgagcaggn nnnnnnnnnc gagaagccga tcgccgcagc
     1321 gaggaagccg ttcatcgtat gcaacaccgt gtcccagaac gggaagatct catagaacga
     1381 actgatctcg ccaagaatct cagccgagaa cacgaacacc agcacgatga cttccagcac
     1441 cgaaggcagc tcgatgcgga actgcacctg gatgaacgcc ggcgccaaca gcagcagcaa
     1501 cgtcaggatg cacaggaaca cattttcgta gtcgccgtta aagaactgga gtattgccat
     1561 ggcgagcacc agcatgcgca gcaccacata gaccgcgaat gagcttttgt gttcgcggat
     1621 ttccatgccc acggctcggc tcatggtttt ccagcgtacg cgccaggacg gtttgcgttg
     1681 tgcgtcgtgc tgttgcgacn nnnnnnnnng tttccgggtc atccttacct ctttctcgca
     1741 ttgttctcgc gccagtctat ccgaacctgc tcacaggcaa tcggcaagaa cgggcgatag
     1801 tctggccgca cgaaccgaac ggaaaccgat actgtctcga ttgtgggcca cgcgaatcat
     1861 ccgacaatgc tcgatgaggc cattacgccg gctcctcatg gctactggtc gtagggctgg
     1921 caatcatggc catgccggta gtttgggcta tcgtgaggtg tgcgagatgg gctttgtgcc
     1981 ggctggcagc aaatccaagc agcgaaacaa cggaatgaac gaagcgaaag gaacggtatc
     2041 tgatgactcc gattgaattg tcaacccgac gccgggttac gggactgact tcggatgagt
     2101 tcgccaggct gttcgtctct ttgcagcgtg aagtggacgg cgaagccgcg cattggagcg
     2161 ccacggacgt cgaatcctgg gagctggtcg gcccgccggt gaccgccgaa cagcatctca
     2221 tcgacacgtt cgccgcggcc aacgaattcg tggccacgct tgcggaccgt ctgtaccaca
     2281 cgattatgcc agtcacggaa gaatcggtca tcaccgcata cgctgatgac ctcacattct
     2341 gggcatcgca tccggacctt ggcggagtgc cgtgcgcatt atggaacatc gccgcactgc
     2401 aggcggccga atgggcccgc aaggaaaccg gcatcgcctg tgaactggac gtgcgcgaac
     2461 cgctggtgtg acagctgagc tcaaacatgc gtaggtaacg gcatggtttg aggactatgg
     2521 tgttgcgtta tcggtcggaa aggatgtgat ggtggggcgt catagcaaaa aagcgcgtgg
     2581 ccagtcgagg agatatgcgc gaaagccctc cgtttggagc gaaattcgcg gctatctgct
     2641 gacagtgatc gcggtgatgc tggtcgtgtt gctgggacgt accttcgtgt tcaacgtata
     2701 tgtgattcca tcccgcagca tggaagacac gttgcaaatc ggcgaccgcg tatttgcctc
     2761 gcgattgact ccgagactct ttacactgca ccgtggcgac atcatcgtgt tcaaagatcc
     2821 cgcagactgg atggaggggg agcagcttcc caccaatctg atgtcgataa tcgacagcaa
     2881 tcgttaccta atcaaacgtg tcatcggtct gcccggcgat accgtcgcct gcaagggatc
     2941 cggggagccc atcacggtca acggcaaacc cattgatgaa agcgcttatc ttaagtccgg
     3001 cgtgaatcca agcgacagcc ctttttccgt aaccgttact gacggcaacg tgttcgtgct
     3061 tggtgataat cgctccaatt ccagagattc tcgataccat cttgacgacg gcaacaatgg
     3121 gctagtgcca tacgacgata tccaaggtgt ggcgttgttc cgcttctggc catttacgag
     3181 aatcggcata ctgaactgag tttggcgtga atccacgagt tgatacgtgc cgcacggtcc
     3241 ggtgcatcct gcagccggac cgttaccgga aacctccccc cgaaatctga cgacccccct
     3301 gtctcacagc tatcgtacag ctccgatacg ggaaacgtgc gggtgattcc ataacgacta
     3361 cgtacttcag gaagtgacgt gtatcacact atggtcatgt gttcagtcgc gcgcgaccca
     3421 aagcgtatcg aggtcgtcgg cctcggtcgc ctttttgcgc ttgcgtaccc actgttgcag
     3481 gaaatacgcc ccatagcagg ccacgatgaa cacgagctcg gccacgagcg cgaaccgttg
     3541 ctccacgtcg aaaatcacca atacgaacga tgcggtgcac aggatgaacg ccgcccaaga
     3601 cacgtacgga tagccgggcg tgcggtattt gagttcgctg gtggaatggc cggactccag
     3661 ccaacggcga cggaacacga tctggcagta ggcgatggcg atccacacca ccacggcgga
     3721 caggccggaa agcgccacca gcaccaggta cacagtcgag gcggcgatca cggaagacag
     3781 gagcgccagc aggccaccgg ccatcgcggc gcacaaggcg agcatcggca cgccgcgacg
     3841 gttcgttttg gcgaaccagc gcggaatcat gccttcgttg cccatcgacc acaccatgcg
     3901 cgtggaggcg tacaggccgg aattcgaggc ggaaagcacg gccgtcagca ccacgaaatt
     3961 catgatgtcg cccgcatagg ggatgccgat gctgttgaag acgagcacga acgggctttc
     4021 gcccacgccg gcctggcgcc acgggatcag cgcgcacatc acggtgatcg agccgatgaa
     4081 gaacagcacc aggcgccaca gcgtggtgtg gatggccttc ggcacggcgg tttgcgggtc
     4141 ccttgtctca ccggcggtca cgccgatgag ctcggtgccg gagaacgcga agttgacggt
     4201 cagaatcgtg gcgaacactg gcatgaagcc gttggggaag acgccgtcct tgatgagatt
     4261 gccgaacatc ggggcatgtt ggtagccggc gaccgggatg acgccgaaga tggcgagcga
     4321 tccgatgacg atgaacgcgc agatagcgaa caccttgatc gaggcgaacc agaattccgc
     4381 ttcggcgaag agccgcaccg acaaggcgtt taaggtgaag nnnnnnnnnn gtcgtccagt
     4441 tctgcactcc cggagagtca ttgaagattc cgaagatcat cagggagcca gaaatcagga
     4501 agatgatgac agtgatgacc ttgatcaggg cgaaccagaa ctccgcctcg gcgaagaaac
     4561 gcaccgacaa ggcgttcagg gtgaagatga gtgtgatgca cgccgcgctc cacgcccatg
     4621 tgggactgtc cgggaaccag cgttgcatga gcaggccggc ggcggtgaat tcggagccga
     4681 gcgccaccgt ccaggtgagc caatactgga ttgcgatcac gaagccggtg ccagggccga
     4741 tgaacttttc ggcatacacg tggaaggagc cggtcactgg catggccacg gacagttcgc
     4801 cgagcgtgag catgaccagg tagacgatca gcgcgccgat gctgtatgcc aggatcgtgc
     4861 cgatggggcc cgcctgctgg atggtgtatc cggagctcag gaacaggccg gtgccgatta
     4921 cgccgcctaa tgagatcatc tgcaggtggc gggattccat gccgcgcttc aggttggtat
     4981 gtagcgcgtg ctcgtctttg ccatcatgtg acga
//
LOCUS       DAWBMW010000109         4999 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_3660_length_4999_cov_2.213592, whole genome shotgun sequence.
ACCESSION   DAWBMW010000109 DAWBMW010000000
VERSION     DAWBMW010000109.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 4999)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4999)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4999
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3660_length_4999_cov_2.213592"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1177..1186
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          920 a         1557 c         1511 g         1001 t
ORIGIN      
        1 agagaaagga gtaccgtggg ctgctcgacg cggacggcag gggcacaatg ggggcggtcc
       61 gctatccgag gctgcacatc agcctgccgg tgcgccacgg cacggacgcc gccacgctgg
      121 agaagtcggc ggggcacctc tacgggacgt cgctgccggt gggggacgaa tccacgcacg
      181 cggtcgtcgc ggcccaccgg ggtctggcgg acaggctgat gttcacgaac ctcgggctcg
      241 ccaagaaagg cgacctgttc gagatcgacg tgcaggggga gcggctgcgt tacaaggtga
      301 cggacatcag ggtcaacggg ccggacgacg tgaagccgct cgccgtcgag aagggacggg
      361 acctcgtgac cctctacacc tgcacgccat acggggtgaa cacgcagagg ctcctcgtca
      421 ccggcgagcg cacggatgcg ccttcgggcg gcgaccccgg tccggtggag cggctgcccg
      481 actggctggt gccgctgtgg ggtctgctgg gggtgttggt cggcgggacc gtcagaggac
      541 cgaagagacg accgggaagg cacgccaaaa gccaccccta tgcaacggac atcttcgaga
      601 ggttcatcca gcacatactg gaccaagtag ccaacgacag tctctctgaa gagtcagctc
      661 tggagacagg ttacttctga cggatgcctt ggcttgcaca ggctaaggca tgacaagcca
      721 tatcgaggag ccgccgttgc gcgacaaact gaccctgacc gtgcccgagg ccggggcgct
      781 ctccggcata cccgcgaggg tggtgagggc cgccgtgctc aacggtgaca tgcccgcctg
      841 ctacgcgggc tccacgacca tgcgcatccg ccgcaccgat ctggacgagt gggtggcgaa
      901 cctgccggtt gacccgccga cgccgaagtg actgggggat aggcgataca caaaagggac
      961 cgacccgttg acctacgggc cggtccttta tatataagga ctaatcggtc agcctcagcg
     1021 caccggtcgc gccctcgctg gccttcagca aagcctcggt gtccgcgtgc aggtacacgt
     1081 tgtccatcgc ggcgtcgctg tggcccatca gcgcgagccg ggcgctctcc ggcacgccgg
     1141 cgttggcgaa gagcgtgtcc gccgtgtgcc tcgcggnnnn nnnnnnccgt tcactatgcc
     1201 gattcgcaac agcgccttgt cccagtggaa gtactcgatt cgcctgtcga tgggcgtgcc
     1261 acggccggtg acgaacagca tgggggagtc cacgcctttt tgcctgcgta tggcgagcca
     1321 ttcgtccagc gccccgcaca tcacatcgtc caagaccacg attctctcgc cgctgtccgt
     1381 cttgggcgga cacagccaga agccgttgcc cagatcggtc acgtcctcgc ccagaggcca
     1441 gtccgccttc cttgagtccg ggattcgctg caactggtgg tcgatcacca aggcgcgtct
     1501 gccgttcagc gtcgtcagct cgtccatcgt cagtccgagc cgttcgccct gcctcagtgt
     1561 ccggtggcga aggcgagcag ccagttgaga cgccacatcg ggtcgggttc gttccttatc
     1621 agctccgccg cctgcgcggg ggacaggacc ttcatcggcc tgcgcctgcc cgacaccggg
     1681 cggaccatct tggccggatt cgttcgtatg aggttttcgt cacgcgcggc ctcaagcgcg
     1741 ttcttcagga cggaccaagt gatcgtggcc gtcttcgggc tgtagcactt caggccgtcc
     1801 tgaatggcgg ccatgtcctt gtggcccagc gagctgatgc gcctcgcgcc ggctatcttg
     1861 ttcagccgca cgatggcgtt ctcgtagctt cgcctgctgt tcggacgcag gttggcctcg
     1921 gcccgctcgt ggtaccaagt ctctagccag tccatgaggc gcggcgacgg cgagttcggc
     1981 gtgaagccct tgcgcacgta tgcgtccagc ttcatcctga gcctcgcgtt cgccaccgcc
     2041 ttggtcttgc cttgggccgt gagccgcctc ctgcgcccgg tggccctgtc gggctctatg
     2101 tctatcgcgg cgtgccagat tccatcggcg cttctgtatg tcgagccgcc gccacccatt
     2161 gccatcattg gtctcccatc gtgtgaaatg cgtagccaaa tgcgtagcca caacgggcca
     2221 cccagaggca tccacagtta cctgtgcgaa atgaagaccg ttggaattaa gccgtttcta
     2281 agattcccga atagttagcc gataatgtac gttatgtcag attagttatc cttgacaagg
     2341 attcggtcgc ctgtatcaac tagtccacgc ataccaaggc cgacagccaa aggagcaccg
     2401 gagcatacca ggccgacagc caaaggagca ccggagcgac caccgcaatt gcaagaatcg
     2461 ccgtgccttt cagccaatcc ttgtcatctc gtttccagtg gaatgctcgc caaatgaacc
     2521 atatcagcat gggaatctca tacagcgaca gcaatggcaa accgatgaaa accaggtcga
     2581 agcccgcggc gcaggctcca tcgcatccca tcatgaagaa gtcctcgccg ttgtgtttca
     2641 gtgtgtttgg aaataacggt ttacggcttc ttcgatctgc tcgggcttct cgatgatttc
     2701 gtaggcaccg tgttcttcca tctcaccggc cggagcgtag ccccagcggc agccaatggc
     2761 gtccagattg caggcgtggg cgccgtcgat gtcggtgtag cggtcgccga tcatcacggc
     2821 cttgtccccc gctgcagcat caaaaccgat tttgtcgaag cagtaccgaa tcacctgatc
     2881 cttgtccaaa cgagagttat ccctggatgc gccatagatg ccgtccaaca gctcggtcag
     2941 atggaaatgc tcacagatcg gaacgcattg gtactcgggc ttgcagctgg ccaacgccag
     3001 ataatagccg tcggcacgca gcttgagcag ctgttcgcgg ataccgggaa acacgacgtt
     3061 caccaagcgg ccgggaactt tgttgccggg ctcgttcgga tcatcgaaca cggcgcgatc
     3121 cgcatagtag ctgcggtaga tggtgaccgc ccggtcaagc tcatcctccg gcacatggtt
     3181 gcgtcgcagc gattcgatga tggccggacc aatgaaacga tgcagctcgg catcgtcggg
     3241 aaccgggcga cccaattcct cgaacgtctt gacgacggag gcgatgatgc cgccgtcgga
     3301 cttggtaagc gtgccgtcga ggtccaacag gaccactttc atcggatgct gtgccattga
     3361 aattccactc ccctttgttc atgtcgactg ctttgaatgg tcgtcaaacg atatgcgact
     3421 tgttccaaca atgattatgc ccagtcggga tgactgggca tcaagttcac gtgttgttca
     3481 gtaggagatc actcgtagtc aggaatccaa ccgtcacggt gcatttgcag gcgcttttcg
     3541 agtagggtgg ccagttcctc ttcgctacgg cgttccagca gcatgtccca gtgggtgcgg
     3601 gtgggtttgc tgatctcgtc gccctcacgg tcggcgtcgc cctcacggtg ggccacggaa
     3661 ccacaacggc actcccactc gtccgggatt tcggcaccct cggcaaacgg caggatggtg
     3721 cggtggccct tcggacatac atacgccaca tcgtttcgtg cggcgaaatc gacgttgtcg
     3781 tcggactcca gcgatttcgc tccgatgctc atgcccctga ggctacgttc tgccatatcg
     3841 gcatcctcct tgaaagatct ttaaccctac taatttacgc aacagttcgg acttggccgc
     3901 tattccaagc ttccgacgtc ggctgacaaa ctcccatcaa acggcccccg tcagtccacg
     3961 ccggagtagg ccaccacgcc acgattgacc cggtcggccg cttcgtgggc gaggcctgcc
     4021 agccgctctc ccccgtcgaa cggcagcggc tgggcgacag cgatctgctg cagcacgtca
     4081 gccagtcgtt tggcagtgcg cacgaagtcg ccgccggtca tgtcggtgcc gtacaggcac
     4141 gatcccagcg aaccgccatc cgcccattca tacatgacgt cgagaatacc gaagtccggc
     4201 cgctgcatct cgtccagacc gtgatcctcg cacaggatat cgatgtcttc acaaatgccc
     4261 ttgagcttgg acgaagcgat ggcgatgggc ccggagatac cgcccgggta gtgcctcggt
     4321 tcgctgtctc ccccgcgtct ggcctcgtat accagtgaag agagcacagc ggccagtccg
     4381 ttggcgtcga gcttgtcgaa cgtgccggcg agcagcgcct cgcacagttc caggtcatgt
     4441 tcactgtaga tgcggcgcag cagcgagcct ttttcggtca acgtcatgtc aatgtggccg
     4501 gccgcatctt catgacgttc gaggtaacca agcccggtga gaatatcgca gatgcaatcg
     4561 aactgacgtg ccacggaccc ggtacgggag tcgtagcgat cggtgacgcg ctgcagttcg
     4621 cgggtctcgc gggcccagcg gtggccccac ttgaggtgtt gctggaggtc cgggcagttg
     4681 cggcatggat gctcgtgttc cgccttgcgc aaatcgcgaa tgcgctggtc aaggttctgg
     4741 aaggcgcgtg aacgctcctg atcggtcagg aacacttcgt gcttcagttt gcggcggcct
     4801 tccttttcga tgtcgctcag ctccatacgc agggtcatga actgtttgaa gtcgccgtgt
     4861 tcgcatgcga acgcctgttc gtatccggcc agtgcgttct tcagtgtgtt gatctgcgat
     4921 tccagctgcc aggccgattc gttggcctcc cactgggcaa acgactggtc cagggtaacg
     4981 cgcgcggtct cgtagtcgc
//
LOCUS       DAWBMW010000110         4930 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_3699_length_4930_cov_2.675692, whole genome shotgun sequence.
ACCESSION   DAWBMW010000110 DAWBMW010000000
VERSION     DAWBMW010000110.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 4930)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4930)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4930
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3699_length_4930_cov_2.675692"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    238..247
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1967..1976
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          895 a         1394 c         1629 g          992 t
ORIGIN      
        1 cctcaggatc ggtgtagtgg ccgacgacaa ccttgcgctt gatgccgagg cgggagacga
       61 tgtaaccgaa ctcacggtcg ccgtgggcgg cctggttcag gttcatgaag tccatgtcga
      121 tggtctccca cgggatttcc ttgtggtgct gggtggccag ctgcagcagc ggcttggtca
      181 gcacttcgag gccgcgaatc cacatcttgg ccggggagaa ggtgtggcac caggtgannn
      241 nnnnnnnaac tccttgacac cgtcggagga cttcagggtg ggcttgagga cgatcttggc
      301 cggaatcttg ccggtgttgt tcaggtagtc gaccatttca gcggagtgaa tggcgacctg
      361 gcgcagggct tcttcgccgt acaggtcctg cgaaccaacg ccgaaccaga tttccttgcc
      421 ctcgaagggg ttttccataa ccatagggtt acttccttta cttctttgtt tatgggggtt
      481 tgtttaagtt tcattcgccg accgaggctc ccctcagaga ggggagccaa gacaccaccg
      541 tgctggcgga gtcagcgctg atttgatgtg tgtgtccgca aggcgcggaa ggcgaaggcg
      601 tacgaaggta cgtcgagcct tccgtaacgc ggcggacact ccatcagatc agtgctggcc
      661 gtagacgttc tggtagcggt cgttaagctt atcgatgtcg gcctggtcga tctcgatgat
      721 gtcaccgagc tgacgggcgg cccacatggt gtgagcgact tcctcggtca tggcagcggc
      781 cttgacggcg ccttcagcat ccttgccgat ggtgaagggg ccatggttct gcatgaggac
      841 ggccggggac ttcgggtact tcttgagggt ctcgacgaca ccctcgccga tcgcctcgga
      901 gccgatcaga cggaacgggc caaccggcac cgggccgccg aactcgtcgc ccatcatcgt
      961 caggccgcac gggatgttct gaccagtggc agcccaagcg gtggcgtagg tggagtgggt
     1021 gtgcacgaca ccgtagacgt cgggcatgtt gcggtagatg taggcgtggg aagcggtgtc
     1081 cgaggacggg gcttcggtgc cgtcgaccac gttgccgtca aggtcgcaca cgaccatgga
     1141 ggacggggtc aggtactcgt agcgcaggcc ggagggcttg atgacgaaca ggtcggcggt
     1201 gtgcagacgc tgggaaacgt tgcccgcggt ccacacgacc aggttccact tgatcagctg
     1261 ctggtgcagg ttggagacga cctcacgaac ctgcttgacc tcggcgcgaa cctcggggcc
     1321 gtaatcagcc aaagttgcca ttattggttt tcctttccac tgtgatggat ggacgttttt
     1381 tcaaaatctc aaggacctcc ctcaatagag ggaggtcaga aaggcttact tatcttcgag
     1441 gttgatggtt tcgatggcgg cgtgctcgat gggcaggccc ttggtgaagc gggcgaagaa
     1501 gtcctcgaaa ccaaccacat cggaagccac cggcttctcc gtggtggact gggcgtcctt
     1561 gaacacgcaa ccgtcaagga agtcagcgag gttgctgtga tcctcgttgg tgttccacag
     1621 gtagttggcg agcacggcca taccccaggc gccaccttcg gcggcggtgg acatgacctt
     1681 gatcggggtg ttgaaggcag cagcgagaat cttctgagcc accttcggag tggcgaagat
     1741 gccgccgtgg cccacgagag agtcgacctg cacgcgctcc tgcttggtca tcacgtccat
     1801 gccgatcttg accggggaga acgcggagaa cagctgggcg cgcatgaagt tgccgagggt
     1861 catgttggct tcggggctgc gggcgaacag cgggcgacct tcaggcagac cggccaggaa
     1921 ttcgccggaa cggaacgggt agttcagcag gccgccgcag ttggcgnnnn nnnnnnatac
     1981 cggcgtcaac cggctggccg atggccttgg cgaactggcc gaacaggccg acccaagcgt
     2041 tgaggtcgga ggtgaagttg ttggcgtggc tcataccggc cagatcgccg gccggggtgg
     2101 tgaccaagtc aacctcaggg tggagggcct tgagcttgtg ctccagaacc accatggcga
     2161 agatggaggt gccggcggag acgttaccag tgcgtacgcg cacggagttg gtggctacca
     2221 tcccagtacc ggcgtcgccc tcaggcgggg cgagcacgat gccgggcttc agggcgccgg
     2281 acggatcgag cagcttggcg ccttcctcgg tcagctcgcc agccggagtg ccggccacga
     2341 gcggctcagg aagcagattc tccagcttcc acggctgggc tgcgacctca ggtagagcgt
     2401 cgaacttctc aatgaatgcg gtttcgtaag tgtgagtggt cgggtcaatc gggaacatgc
     2461 cggaggcatc gcccacgccg aggaccttct taccggtgag cttccagtgc acgtaaccgg
     2521 cgagtgtggt gaagtaggcc accttggaga cgtgctcttc cttgttgagc acggcctggt
     2581 acaggtgggc gatggaccaa cgctcgggga tgttgtactg gaacagctcg gagagcttct
     2641 tgtgagcttc ggaggtgttg gtgttctgcc aggtgcggaa cggcaccagc agttcgccgg
     2701 cctcgtcgaa tgcgaggtaa ccgtgcatca tggcggagaa gccgatgtgg ccgacgtgcg
     2761 tgagcttctc gccataggcg gtctcgacgt cgttggccat ggaggcgtaa gcggactgca
     2821 aaccgcccca gatctcctcg gtggtgtagc tccacagacc atcttccaga tgggaggccc
     2881 agctgtagtc gccggaggcg atggtctgga agttgtcgtc gatgaggacg gccttgatgc
     2941 gggtggagcc gaattcgatg cccagcgtgg tcttgccagc gcgaatcttc tcggcgatgt
     3001 gggcgacctg ctcggccgcg ttgtcggttg ctgccatatc tttcctttgc cttggagcgc
     3061 cggcgttgtt atccccttcc ttggggaggg tgttagcgct cacaatttcc aaaaaacatt
     3121 gtaccgataa cctgcattcg acacaactta taccgtgtca cgcatcataa cggtttgata
     3181 acagggccaa tttaccgccg aggagccggc cccaaggacc gccgagcacg cacctcggcg
     3241 ggaatcagcc ccatgccgtg ctgggtggtc aggaaggtcg gctcgccgcc ctcccccagc
     3301 aagcgcagcg tctctcgcat ggcggccacg ccgagcccct cgaaatcggg atgcacggtg
     3361 gtcagcggtg ggtacatgtt atccacgcca gacatgtcgt cgaatccgac cacgctgatg
     3421 tcttgcggaa tccgaagccc atgctcatgc aacgcgcgca tgatgccaac ggcctgattg
     3481 tcgttcgcgg cgacgaccgc ggtgggcaac gccgcgcctc gacgcccata ctcatcaatg
     3541 agatgattca tgtgcgcata tgcctcagag gcatcccacg tatggcagcg cacgatctgt
     3601 gaatcgatgg atcgggaggc ggcgagcttg cgccacgcat ccagtcgggt gtgggcgtcg
     3661 cgccattcgt tgggaccggc aaaatacaga gccgatttgt gcccgtattc gcccagcaag
     3721 gccaccactt tggccattgc accccactgg tcgataccga ccagcgcagt gcgacgcttg
     3781 ttttcggtgg agataaggcc caatccctca cgcacggtca tttggccgtg ggtggcggtg
     3841 acgatgacgc gcggctgagt gactcgtgca cggcaggcgg cggcgaacat cacgtcggtc
     3901 ggggtcagga agatgaaggc gtcgacgttc tgctcgttga aggtgtcgca caggttgtcg
     3961 aagtcggcct gcgcgcacaa ggcctcgtgg accatcatca cgctcatgaa cagaccgtgc
     4021 tcgcgggcca tggattcgat ggaggagatg gaggaaatcg ggccgaagaa tttgaggcct
     4081 ccggcgatca gccctatggt gcgcgaacgg cgggaagcca gtgcgcgcgc ggagttggag
     4141 gggcggtagc cgagttggtc aatggcggcc tggactcgcg cacgggtggc gtcggaaacg
     4201 tccggcgagt cattgatcac acgggagacg gtttggtggc tgactccggc gagcttggcc
     4261 acttcgaaca tggacggacg tttgcttggc ttgcggctgg tcaccatatt gcctaacctc
     4321 cccgctggcg gctggcatcc atgcacggcc agtcttgtac cttaatcaat actgacttta
     4381 gcgcgtccac aacagttttg cgttagcgct cacactgtcg tgtcgcacgc ggctcaggtc
     4441 aggtgccatg agacacgatg gagcggagtc ggcccgtgct cggcgatggc ggtacggtgt
     4501 gcggcggaac catagccctt gttatgagcc cattcgtagg cggcgtaacg tgggttgctt
     4561 tgcgcgaggt cgaccatcag ccggtctcgg gtcactttgg cgatgaccgc cgcggtggcg
     4621 acggtggcgc aatgctggtc gcctttgact ttggtcgtta tgtcggctgg aatcggcacg
     4681 tccggcgcgt cgaacgtgtt gagcgccttg gtgatgtaat cgctcggccc gtcgagaatt
     4741 ccggcgactt tgggcagagc gcgaacgctt cccccgcaat ttccaatgga ttcccctacg
     4801 gagcctcccg gacgaccttc cacaatctcc cccgcagact cccccgaagc cttttttatc
     4861 gtcgattccc ctccaagccg taagccaagt tcgcgttcga cttggctgag cgcgcgcagg
     4921 gcggccacgc
//
LOCUS       DAWBMW010000111         4910 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_3717_length_4910_cov_3.224511, whole genome shotgun sequence.
ACCESSION   DAWBMW010000111 DAWBMW010000000
VERSION     DAWBMW010000111.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 4910)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4910)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4910
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3717_length_4910_cov_3.224511"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1889..1898
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          992 a         1364 c         1557 g          987 t
ORIGIN      
        1 aaccgccgga aatgtacggg aacgggtggg ccggatccac ggcgagcggc gtcaggaccg
       61 ggaacacctg ctggcggtag tagcgcgaga ggcgctcctg ctcggaggat gtgagcttgt
      121 cccacgtcag caggacgatg cgctccttct ccaattccgg cagaatggtg tcgatcgtgt
      181 aatgtgcgtg ctcgtcttgc agacggtgtg cggtctcgct gatcgcgcgc agctgctgac
      241 gcgggctcag gccggcggca gacggcacgg cgatgcccga gtcgatacgg cgcttcaggc
      301 cggcgacgcg gaccatgaag aactcgtcaa ggttcgaggc gaagatcgcg gcgaagttgg
      361 cgcgctcgag caggggcatg tcttcgttct ccgcacattc gagcacgcgc tggttgaact
      421 tgagccagct gagctcgcgg tcgaagaaac ggtccttggg cagcggtgcc tcgccttcct
      481 gatcgacgcg gcggtcgttg ttgtcgtttt cggcaatatg ctctgcgatt tggctgcgca
      541 gaattgcctt cgagggtgcg tcaaatatct gtgccatagc ttatatcgta agctttggcg
      601 tggccggaaa acgactgaac gcgccagaat tcaacgggtg ttcatgttag ttcggtgttg
      661 gttggttttg agtgtgctag cgtcgctcgc gacacgccgt gagcggcggt gaaacgaacg
      721 aaatcgaaca tcggacgggt tcgatgtgaa cggttcagct cgttttccga tagccagaaa
      781 gtgcgattga acagttgtga taaaaaacgc acaacgactc tctgcaggtt gcgagaaacg
      841 ggagtgatga atagattatg aggtacaaga acaactgaac agagcagagc ccacaaatag
      901 tgtatggtca cgaggactgt tacgttggac gggcattgga aagacttacc gttacaacga
      961 tgatgttgtg cagagattct gctccagtag ttgaacttgt agaaaaggcc cgctccggcg
     1021 ggccttttcg tttgtgtgta cagtggaggg catgacggaa acacagcgta cgccagaggg
     1081 gatgtcgtcg aaacctcgta tcaagccggc cgatgccgaa gacggccgcc cggtctctat
     1141 ctctgcgcgt ctcggacgac tgcaattcca ctattccggc aagttccgcg tgctgcagat
     1201 tgccgacatt caggatgggc cgaaagtctc caaagacacc atcaccctga ttgaggccag
     1261 ccttgatgcc acacggcctg atctggtggt tttctccggc aaccagatcg ccggctacga
     1321 tccggcgttc gccgattcat tccgtaaacg tcgctggtgt gacgaaccta ttgccgaatc
     1381 cgcgctgaac catacccgag cgctggtgcg caaggccatt ggccagttca ctgagccgtt
     1441 ggcggcgcgc ggcattccgt gggccgtgac gtatggcaac catgatttcc aatgcgggct
     1501 aagcaatgtg gaactggacg gtatctatcg tgagttcccc ggttgtgtca atccgccgag
     1561 tgagaccttg ccgaaccaga ttgcatacac ctgtggagcg ggtggcgctg tgcagacgct
     1621 gtctggtgcc actggttccg gtgaaccagg caccttcgcg ctgccggtta tggacgttga
     1681 ccatacgcgc aatgtgctgg gtctggtcat tcttgactcc ggcgattacg tgcatggcgg
     1741 cggcttcggc acaccatcgc ccgcagcgct cgccttcctg aacgccgtgc ccgaccgtat
     1801 cggtgcgaaa tcaatggtgt tccagcacat gccgatgccc gagtattaca acgtgctgaa
     1861 gccggtggcg gccaacgcgg cgtttgccnn nnnnnnnncc atgcagggct atcgttcgca
     1921 cgccgatacc tactacgtgc tggacgaatt gcaaacccag cccggcggat atctgggaga
     1981 aggcatatcc tgccctgata cgtcagacga attcgaattg ctgcgcgagg ggtatttcgg
     2041 cgtcgtagct ggtcatgatc atcgcaacgg tttcgtcggc gaacatgagg gtctgttgct
     2101 tatcgctacg ccaacctgcg gattcaatac gtacggaccg gctccggcca aacgcgccac
     2161 gcgattgatc gaatttgata tccgtcaccc ttacgagccg cgtacgcaat tgctggagtt
     2221 tggcgaactg gtgggcaagc ccagttccaa acgcgcctat acctatgcgg tgaaccagac
     2281 cgcgcctggc gagggcgagg gcgatgattt gctgcgcaag ccgtcgctat ggagtcaact
     2341 gtccggttta ttcaggtaac ccggtttatt caggtaactg ttcctgatag aggcggaaag
     2401 cggcgcgaag tattcgaggt ttcggtggcc ttccgctgtg ttggacgatt agtggcagaa
     2461 atcccgcgtc tctttcgttg tgacgcggga tttctgccgg ttgggctatc ggttggattg
     2521 actggtatgt ttcagcgaac ggctttgacc agtgcctcgg tgaggtactt gccggcggcc
     2581 atcaccagcg gcgcatagcg gcgtccgggg gcggcaccca tgcggccggt cgggccggaa
     2641 atcgagatgg cggcgatgac ctggccggag gcgttgcgga tcggggcgga aatcgagcac
     2701 acgccctctt cgcgttcgtt cacggactcg gaccagccgc gcttgcgtac ggcggccagc
     2761 ttggtggcgg tgaacttggc gtggcgcaat ccctggtgca gacggtcgga atcctcccaa
     2821 gccagaagaa tctgggcggc ggaaccggct tccatcgaca gcatggcacc caccggaatg
     2881 gaatcacgta ggccggaggt gcgttccaca gcggcgatgc acacgcgctg atcgccttgg
     2941 cggcggtaaa tctgcgcaga ctcgccagtg cgatcgagca gggtctgcag gatcgggccg
     3001 gcggcggtca gcagacgatc ctcgcctgct gcggcggcaa gttcggcgaa acgggagccg
     3061 agtacgaatc ggccatgctg gtcgcgcagt acgaaacggt ggcgttccag tgcaatagcc
     3121 aaacgatgtg cggtcggtcg agcgagaccg gtggcggcca caagctggcc cagcgtagac
     3181 gggccggatt ccagtgcgtc aagaatcttt acagttttat caaggacgcc aacgccggaa
     3241 tggatttccc catcgtcctt ggcggtggtg tcttcaaccg gctcggactt ggtcaagctg
     3301 ttgttcaatg gttcttcccg ggaatcagtc ataataaggg cgattatgcc atctcacata
     3361 gtgaaatggc aaatcgggga agtgtctcac agggtggcca tgaagttgaa agggcgttcg
     3421 tggatgccga agagacatgt ctggagcgga accgagtcat gactaggtca tgttcgagtc
     3481 gggtccgagt catattgagg ccatcatttg accgtatctc aatatccggc atgctcatgg
     3541 ccgggcccat cgcaagcaca taggctaata ccacgaacaa ggcgcgaaca caatacctcg
     3601 cgcggaattt tttggaggaa tcactatggg aacgacactg gccgagaagg tctgggccga
     3661 tcatctggtg cgcaagggca gcgacggagc ccctgatttg ctgtacatcg atctgatgct
     3721 catgcatgag gtcaccagcc cgcaggcgtt tgagggcctg cgcttggccg gccgcaagcc
     3781 gcgtcacgtg gatcagctca ttgccaccga ggatcacaac acgccgaccg ttgatatcga
     3841 tcgcccgaac ccggatgaga cgagcgcgtt gcagttgacg actttggaga agaactgcaa
     3901 ggagttcggc gtgcgcctgc acccgctggg cgacgccgat cagggcatcg tccacgcgtt
     3961 cgccccgatt ctgggcctga cccagccggg catgaccatc gtgtgcggtg attcgcatac
     4021 gtcgacgcac ggcgcgttcg gcgcgctggc gttcggcatc ggcacttccg aagtcgaaca
     4081 cgtgatggcc acacagacgc tgtctctgaa gccgttcaag accatggccg tcaacgtcaa
     4141 cggcaagctg cccgcggacg cgacggccaa ggacatcatt ctggcgatca tcgccaagat
     4201 cggcaccggc ggcggccagg gttacgtgat cgaataccgt ggcgaggcga ttcgtaacct
     4261 caccatggac gaacgtatga ccgtgtgcaa tatgtccatc gaggccggtg cgcgtgccgg
     4321 catgatcgcg ccggacgaga ccacgttcga gtacctgaag ggccgtccgc acgcgccgga
     4381 aggcgagctg tgggatcagg ccgtggcgta ctggaagacg ctgaagaccg atgacgatgc
     4441 cgtattcgac aaggtggtgg atattgacgc caccaagctt ggtccatatg tgacgtgggg
     4501 caccaacccc ggccaaggtc tgccgatcac cgcctccgtg ccggagccgg acaagatcgc
     4561 cgacgccacc aagcgtgccg ccgccgaacg tgcgattaca tacatgggtt tgaagccggg
     4621 catgccgatc aaggacatcg ccgtggacac cgtgttcatt ggttcgtgca ccaacggtcg
     4681 tatcgacgat ttgcgccagg ccgccgcgat catgaagggt caccacaagg ccgagaacat
     4741 tcaccgcgtg ctcgtggtgc cggcctcctc ccgtgtgcgt ctgcaggcgg agaaggaagg
     4801 cctcgataag gtgttcaagg actttggtgc cgaatggcgt aatgccggct gctcgatgtg
     4861 cctgggcatg aaccccgaca agctggtacc gaacgagcgc tcgatttcca
//
LOCUS       DAWBMW010000112         4886 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_3734_length_4886_cov_2.387497, whole genome shotgun sequence.
ACCESSION   DAWBMW010000112 DAWBMW010000000
VERSION     DAWBMW010000112.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 4886)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4886)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4886
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3734_length_4886_cov_2.387497"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          977 a         1407 c         1554 g          948 t
ORIGIN      
        1 cgtctcaccc atcccggttt tccgatagtc ctgcgccccc tgaccatgga tgatgaggac
       61 ggctggaacg aagtgcgctg gcgcaacaac gactggctgg cgccttggga atccggtgac
      121 ccgatgcatg gcggctccat cacattcaat caatgggtgc agcggcagcg ccgtaacgag
      181 cagcatggcg tcggcgtgat cttcgccatc gaataccaga tgcgtatcgt cggccagatt
      241 tcgttgggag ccatcgtgta tggtgccatg cgtaccggcg tggttggtta ttgggtcgat
      301 caacggtgcg ccggccgcgg attcgcgcca atggcactgg ccatgcttgc cgattgggcg
      361 ctcggagacc cattcgggcc cgcgttgcat cggctggaaa tcgccattct gccggataat
      421 gcgcgttctt tggctgtggt gcgtaaggtc ggagcccatc atgagggact gcgcgagcgg
      481 tatatgtatg tcaatggcca gtggcgtgac cacgtaacgt ttgcgctgtt ggctgaagat
      541 atggggcaag ggttcgccgc tagactcgcc gccgccggtt ccagataagc gtcgaccgga
      601 agaattccgc tccgacacgc ccggacggaa agccccagtt cgtcggcacg tctcctatcc
      661 ttgactctat gggttatgag tcactgagca ctgtcgtcgt gctggtgatt gtggcgatta
      721 ttatcgttgt atggctgccc gtacgtacgg ccaacggcat gaaacgtgtg gacgagcacc
      781 ggcaggatcg ttattcgcct tccctgcata tcgttgatgc agagaacggc agacgattcg
      841 gagacatcaa gccgcataag gcgaaagggg cagcaatgcc agcatcaaca ccgtcagcga
      901 ggctgacacc tgagcacatc gcccatgtgc gggaactgcg ccgcgcggcc atccgccgcc
      961 ggcagattct cgccgtatgc ctgcttgcca taaccgtgtt ggtgtttgcc gtatcgttcc
     1021 ccctgcattt cagcccgctg cttgctctta ttccgttcgt gctgttactg ctggtgctgg
     1081 tgctgggagc caatgcctca cgccaggccc ggcaatggga gcgcaaggtg gtccgctacg
     1141 agcggactca tagcggtacg ggctggtcca agaaaccgtc tgccgagtcc aaggacagca
     1201 agcgtgtgaa caaggccgaa ccggttgcgg ccgtaactgc gcctgccaag gagctttccg
     1261 agcatgttga ggatgctgcc accgaggtca tggaacagcg gcagattcgc cgcgcgttac
     1321 gcgatgccga gattgagcag gccaaggcca aggcgttgcg tcagtccgcc gccgcatatc
     1381 aggccgcggc ggagcacgcc aaacagtctg agaagtctga tactgcacag tccgccgccg
     1441 atcagcctgc cgccgaaccg tctgctatgg tcgatgaatc cgctgaccgt aagtccgctg
     1501 ccgaaaccag tgaatcttcc gccgcgcccg atgcctccgt tcgcacagac aagtcgattg
     1561 agtcggacaa gtccgtcgcc cctcgagttg aaccatcgct tacggtgcgt gacgagcgcg
     1621 atgagcgcga cgatgccgct gccgctgccg atgccacgtc ggaactggtc agcgtacgcc
     1681 ctgcccgagc actggacgtc ttcgatatgt ccacctcgca ggatctcatc tccttcactt
     1741 tgggtgggga acataacgcc gacaacgctc cggaaagctt ggagatcaag tcgacccgtc
     1801 aggtgtccaa ggcggaacct gtcgaacccg cagtggcgga aaagttgatt gacgaggccc
     1861 gagccgtcaa ggccgcggac gacgccaagg ctgtcagcgc cggaaacgct gtagacactg
     1921 agccggagca gcgcgatgat gccgatacgg acgccgatga cgaggctgca aacgccgccc
     1981 agcgagccgc attccacgaa tccgaggagc gtgccgacgt cgaagcgcca gcggcgacca
     2041 ccgattcgtt gggcgcaggc ctggactcca ttcttgcccg ccgcggcaac tgagcgttgc
     2101 gattcgacga ttttctctca cagcgttagc actcgagttg gcagagtgct aataatcgcg
     2161 ctacgatgtg tcctagcgca aagggacaac gcaagtgatc gtcagcctct tgaggcgttc
     2221 tggcgcggca ttatcgaact acaagtagat acgaggtgac ccacagtgtc gattaagctc
     2281 acaccgttgg aagacaagat catcgtcaag caggccgagg cccagaccca gaccgcttcc
     2341 ggtctgtaca ttccggacaa tgccaaggag aagccgcagc agggtgaagt cctggccgtt
     2401 ggcccgggcc gtcgtgacga caagggcgag cgcatcccga tggatgtcaa ggtcggcgac
     2461 aaggtgctgt actccaagta cggcggcacc gaagtccact acgagggtga ggactacctc
     2521 atcgtcggcg cccgcgacat tctcgccatc ctcggctgat tagtcgccta gcgaacttca
     2581 acaaccccga ctgttcccgc ggatagtcgg ggttttctct tgctaggcgg aaaggggctc
     2641 cccctcagag aggggagccg aaagctgctc ccaccaaacg cccagccatg cctgctatgc
     2701 ataaaggtgc atgcgcgcaa cgtagttgag ttcggcgttg caccgcatct acactgggta
     2761 acggattggg gtccctatgg aagaggctca gttggcgact tggccgagcg gcttaagaca
     2821 ttttgcacaa gacggcagta gatcgacggt atgttgtttt ttccgtgtcg caggttcgtg
     2881 gtgtcgtcgc gctgtgccaa tggttgtctc tctaccgacg ctgagtgccg tggcatgagg
     2941 ccagcgtcgg cagtgggcca cggaatggcg atgtgctgat gctgagccgc gttggcgtcg
     3001 ttatagggcc agtttttgga ccattgccga cggcaaatcg gcgttttgtg tcggcgacgg
     3061 tccaaaaact ggcggtctgc tgacgataag tgccgtcatc gtcggcagac cgccaggtat
     3121 tgtccccgtg ccgacgtaaa aagccctgcc atcgtcgtca ccgagccatt ttgtgactca
     3181 ctgacggcgg cagcagggcg gaagaggccg aaatcatgcg cgcggtgcgg tctcacacct
     3241 cgaatccggc ctccaccagc gcgtgatcga tgtcgcgaat caaatcgtcc gtatcctcca
     3301 gacccacggc cagacggaag aagccgcggt ggaactcagg cgggtacagg tactggcgct
     3361 cgtcatcctc gccgaggaac acgatcagcg attcgtcatg gccgagcgag acggccgacg
     3421 taatcacatt gagcttggaa acgaaccggt tgtgcccgtc gtggtcggtg tcgaggccga
     3481 acgacagcac gccgccgaac ccggagtccg ggcgggccag ctggctggcc gccacctcgt
     3541 ggtgcaggtg cgattccaga ccggggtacg ccacgaaacg caccacatcc agcgattcga
     3601 gatgcttggc aatcgccaag gccaaggcgt tgtgctggcg gatgcgcagt ggcagcgtca
     3661 ccgagccgcg attgatcagc caggcattga acggcgagat gatgccgccg aagttcacct
     3721 gcgcggtgaa gcggatctgg tccgtgcgcg ccttggtggt ggcgatcgag ccgcccagcg
     3781 cgtcgccatg gccgttgatg tatttggtga gcgattcgat gacgatatcc gcgccgagat
     3841 cgagcgggcg cacgttgtac ggcgtgtcca ccgaaaccag aatgtccggg ttcgcttcgt
     3901 gggccagttt ggcgatggtg gcgatgtccg agaccttgag cgtcgggttc gccggtgtct
     3961 cgatatgcac cagcttggtg ttggggcgaa gcgccgcgcg tacgttctcg ggatcggagg
     4021 tatccaggat ggaggtttcg atgccgtact tctccggcag aatctggttc aacaggcggt
     4081 aggcggcgat ataggtggtg tcggagaaga tcgcgtggtc gcctcggttg aggaacgtgg
     4141 tgaacgtggc ggccagcgcg gccacgccgg aagcgagcac caccgcatcc tcggcacctt
     4201 caagacgggc cagcttttct tccagatagc gctggttcgg atgcccgttg cgcgtgtaga
     4261 ggttcacgtc cgaactggac cagttgatgt ccgaggggtc gtagggcagc gcgtaggcgt
     4321 tcgccagcgt gatggggcga cggatcgcgc cggtttcggc gtcgaccccg ttaccgaggt
     4381 gaatggccag cgtgccgaat ccgagcggct gatcgtcgaa tgattgtccg tgatgtacaa
     4441 gatcagaagc catgcgccca gcatgccagc atgcgccgtg ccgcgcaaac aggcgacatc
     4501 gctcgaaaac cgcgccattg caaggttcta gagcaccata taagaaaaaa tgattgggtg
     4561 aagtgcgggg tgcagccttg gtcggggctg tttgcagggc cgaagcgggc cctgaaaaca
     4621 cgctgtgaga cacgccgata aaaccgcaga attccgcggt aaaacaaggt tcgcgtaggg
     4681 gccgatttgc atacctggaa aatctgtggt tatatagcca agttgcctgt tgaggtcaaa
     4741 caccgcggcg ggaaacagac tggcggattt cccaagcggt caaagggagc tgactgtaaa
     4801 tcagccgctt cgtgcttcag tggttcgaat ccactatccg ccacgccccg atagctcagt
     4861 ggtagagcac ttccttggta aggaag
//
LOCUS       DAWBMW010000113         4849 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_3763_length_4849_cov_2.766583, whole genome shotgun sequence.
ACCESSION   DAWBMW010000113 DAWBMW010000000
VERSION     DAWBMW010000113.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 4849)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4849)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4849
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3763_length_4849_cov_2.766583"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    212..221
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    860..869
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          889 a         1488 c         1482 g          970 t
ORIGIN      
        1 gcacggcacc gatcacatac tgccaccatg gtgttcgccg tgttttcatg tccggcctcc
       61 ttatcgttgc gttgctgttt gctgtttgcg aacggccctc gcgcctgggc tgttcatctt
      121 gatttggttt tcgtcactgc cgtgcccggc tgttcgaggc cggagactgg tccgtcaccg
      181 tctggtttct agcctaccgt tcgtaactgg tnnnnnnnnn ngctcaccac aatctgaccg
      241 ccataccagc cggcgagcgt tgcgcccgcc aatcccagtg cctttgccaa caccagcgta
      301 tacaccgcga tagtgggatt gatgaagcta tgcggacgtt tcttggggtc ggtattcgcg
      361 tatttatgca cttgcagggc caagatcagc acgataacgc ccagcacggc cagcaatccg
      421 gtcacaatcc aaggcgcacc aatcagcgac agtcccgagc tttcgccgta ggaggccaga
      481 ccgatacccg ccaacaggtc aactgttcga cagtatccag ttgtccgata gcaatccgac
      541 ctgtccggca tccggtgcca tggccaaaag gaaggccaca gggtcgccgc ccagagaagc
      601 attcgggtcc atatccagcc aaggtgccaa taccgacgca tggttacggg ccgacggcca
      661 aggaatgctg cagttgggct cgtcggaagt cacgccttcc atgtcgacaa ggtcaaggtc
      721 gactgccaga tcatgaacac tttcgatgct gcccaatacg gcaatcaggt cggcgggcgg
      781 caacttggtt tcgatttgga tgaccgcgct catcgcgtcc gggccgtcga aatggctgac
      841 atggtagagc ggcgagatgn nnnnnnnnng ccctgtacct gattgccagg aacactgtcg
      901 atggaaacga tggtttcgcg gaacagccgt tcggcatctt gcgaggtgga atccagcgaa
      961 ataatcgcct tgcgtgagac gggtgccgac tccgacgctg atttttgctt gactgcatgc
     1021 ccgccgagca tccagtcttc actctcgagc tgcacggcgt tacggtccga tgcggcggcc
     1081 agcaaatcgg ctacaggccc ctcatgggct ccggcgagtt tggcgttcgg attcaatgcc
     1141 aaccaaggag ccagcacaaa cgcacgctgc caagcacgcg gatggggcaa tgccagatcc
     1201 ggatccgcgc tggtgatgcc atcaaagtcg ataatgtcca agtccaacgg cctggaatcc
     1261 caatgattct cacgaattcg tccatggttc gcttcgatgt tttgcaacgc ggcaagcaat
     1321 tcgtggctgc ccattgtggt ttcaagctcc accacggcgt tcaggaaatc cggcgtaccg
     1381 tcggccatgc cccacgcggc cgtgcggtaa agcggtgaga taccggtcac ctgtgtgcca
     1441 ggcaacgcgt caatctcccg aacggcggct cgcaaggtgg attccacctc gcccacattg
     1501 ccgcccaagg ccaccaccgc gttatggatg gcatgctctt cagcagtacg tccattatcg
     1561 gtggcacgca cgcgcgagat ggagaccgat acatctgcaa aagccacgac gatgggtgcg
     1621 tgaggcttgt gcacggtgac gtcgatggag gcgacggccg gcgcatcggc aaggatctcg
     1681 tccgcgatac gttgggccag acgctcgatc aggtccacat gagggccttc gatgacagcc
     1741 gtgatgtcct tggccacacg gccgtaatcg atggtgtcgt tgagatcgtc ggattgaccg
     1801 gctgcggcca gatcaaggtg cagggtgaca tccacgacga aggtttgtgg ccgttcgtgt
     1861 tcgaaggcga gcacaccgtg ggtgccgttg gcatgtacgc cggtaagtgt gatggtgtcc
     1921 attggtaagg ctttctgctc aaatgctccc ctcagtcgcc ttcgacgcca gctcccctca
     1981 cccgagggga gccaaggcct agaagcaacc aaaacaggga gccaggggaa tcatgcgtat
     2041 tcgcgccagg tgttgccggc gatgacggcg gcgcggcttt tggccacgtc gtgtacgcgc
     2101 acggcccatg cgccatgttc ggcggacagg gcggacaagg ctgcggtcac gtcatcgcgc
     2161 tgggccatga tcggcccatc agcaccggca gcgcccgcct cgttaagcat cgccgaaatg
     2221 aagcgtttgc gggactgacc gatcagcacc ggatatccag tggcgcggaa ggtctcgagt
     2281 ccggtcagca gcggcaggtt gtgctcgatg ccgggcttgg agaagcccag gcccgggtcg
     2341 atgataatgc gctcaggctt tacgccggcg gccagcacac cgtcgacttg gcgcatgagt
     2401 tcgtcgtgta cgtcggtcaa tacaccgtgc tcgtagacgg aggtgtcttg atcggggttc
     2461 gcgcccttgg agccggcgag ccaaccgcgc caatgctgca cgatgtacag gcagtcgtgg
     2521 tcggcgacca catgcggcag ttcggcatcc agcgtgccac cggaaacgtc gttgatgatc
     2581 tgggcgccct cgctcagcgc agcggcggcc acggatgcgc gcgtggtgtc gatggacagt
     2641 atggcaccgg ccggaatcaa tgtcttgacg gcaccggtga tgcgggccag ctcgtcggcc
     2701 tcggcaacgc gcttggcgcc ggggcgcgtg gattcggctc cgatgtcgat gatgtccgcg
     2761 ccggcggcca tcatgtcgcg cccgtgctgg gccgccttgg ccgggtccag ccacaggccg
     2821 ccgtccgagg acgagtcctc ggtgatgttg agcacaccca tcaccatggt gtgcgtactg
     2881 tcatgcaacg cctgcaaatc agtcattatg tttccccttc tatgtaggtc ccaagtgagt
     2941 tccctatgat gagggagctt agtagacact atttttccaa aatgaggctc atggcttcgg
     3001 ctcgggtggc cggattgcgc agcatgccgc gcaccgcaga ggtcaccgtg cgggcggaag
     3061 acttcttgat gccgcgcata gacatgcaca ggtgctcgca ttcggtcacc acaatcacac
     3121 cacgtgcgcc cgcatactcg accagcgcgt cggcgatctg ctgggtcaaa cgctcctgca
     3181 cctgcggccg gcgagcatac acctcgacca gtcgggcgag cttgctcagc cccatcaccc
     3241 cgtccttggc gggaatatag cccacatggg ccacgccgtg gaacggcagc aggtgatgct
     3301 cgcacaccga atacagttcg atgtccttga ccagcaccag ttcgtcggta tccacgtcga
     3361 agtgcttctc cagcacatcg gccggggaag cctgcagacc ggcgaacagc tcgcggcacg
     3421 cgcgggcgat acggtcaggc gtttccacca gtccctcgcg ctcgggatcc tcgccaatcg
     3481 acttcaggaa caggcgtacg gcttcgcgca cgccttcctc gtcgtatgtg cgtttctccc
     3541 gctggcaaga ctctatatat tcgttcatct catctctccc ttgtcattgt ccacaacgac
     3601 gtcggcccgg cctgcgtacg tgagaaacca catgaacagg tcgggccgaa tcgtcatcgt
     3661 gtctactctt ccggcttcat gccgaccgag cgcttgaggc tttcgggaat ctccaccggc
     3721 ggcttgtccg agtcggggcg gcggtcgttg gacagccaca ccgggcggac cggcgccttc
     3781 ttgatcggcg cgaagatctc cgcaagctcc ttctcgttca gagtttcctt gaccagcagc
     3841 tgacggacca actcgtcgag gatatcgcgg ttgtcgttga tgatggtcca cgcctcggta
     3901 tgcgcggtct ccaccagctt caatacttcg tcatcgatca cttcggcggt gcgatcggag
     3961 tacttgcgcg gctgcagacc atccatcaca gtggtctgat cgtcgtcgga accccacttg
     4021 atggcgccga gcttgtcgga gaagccgtat tcgatcacca tcgtacgggc gatgctcgta
     4081 gccttctcga tatcgttcga agcaccggta gtcgggtcgt ggaagacgat ttcctcggcg
     4141 gtgcggccac ccatcgcgta ggccatctgg tcgagcagct ggttgcgcga ctgcgagtag
     4201 cggtcggagg tcggcatgac ggcggtgtag ccgagggcgc ggcctcgcgg cagaatcgtc
     4261 accttggtca ccggatcggt gttgttcaag gcggcggcca ccagcgcatg gccaccctcg
     4321 tggtatgcgg tgttgcgcag ctcttccaga gccatgccct tagacttgcg cttcgggccg
     4381 gcctgaacgc ggtcgatggc ctcgtcgatg gcgcggttgt cgatcaactg ggcaccggca
     4441 cgggcgcaca gcaacgcggc ttcgttgagc acgttggcca aatcggcacc ggtgaagccc
     4501 ggcgtgcgca cggcgaccat atgcaggtcg acgtccggta cgaacggctt gcccttggcg
     4561 tgcaccctca gaattgcctc acggccttcc agatcaggcg cggccacacc tacctgacgg
     4621 tcgaatcgac ccggacgcag cagagcgggg tcaagcacgt cagggcggtt ggtggcggcg
     4681 atgatgatga ggttggtgtc gttgtcgaaa ccgtccatct cgacgagcag ctggttcagc
     4741 gtctgctcgc gctcgtcgtg gccaccgccc ataccggagc cacgcttgcg acccacggca
     4801 tcgatctcat cgatgaagat gatggccgga gcgttcttct tggcctcat
//
LOCUS       DAWBMW010000114         4800 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_3805_length_4800_cov_2.741623, whole genome shotgun sequence.
ACCESSION   DAWBMW010000114 DAWBMW010000000
VERSION     DAWBMW010000114.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 4800)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4800)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4800
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3805_length_4800_cov_2.741623"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    228..237
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    591..600
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          849 a         1303 c         1622 g         1006 t
ORIGIN      
        1 tctggcgtcc aaggagaata tcgagtcgat cgggcggctt gcgcatgatc tgaagcatca
       61 gattgcggcg ttgcgcgcgg aggtggaccc ggagcatgcg gcggccggct tcgagcagct
      121 ggaggcttcg gtggcgcaag tcagcgccca gcagcattcc ggcaatccgg tgctggacgt
      181 gattctgacc accaaaatgc gcacgtgcgc ggatcgcggc atcacgcnnn nnnnnnnggg
      241 catgtcgtcg atggatatcg cctcgctgtt tggcaacgcg ctggacaacg cgatcgaggc
      301 gaccagcaaa ctgcccgacc cgcagcagcg gctgattcgg ctcgcgctgt acgggcaggg
      361 gcagttcacg gtgattcgcg tggaaaacta ctacgattcc gcgctcaaga aggattcgac
      421 cggcgcgctg cgcaccacca agcgtgattc gtcgcggcac gggttcggcg tgaagtcaat
      481 ccggcacatc gccatgcagt acggcggcga agtgacaatt cgcaccgagg accactggtt
      541 tgtgctgacg gtgctgctgc cgaggtgaat cagaagtccc agtcgtcgtc nnnnnnnnnn
      601 cgggcgagcc gtcgtcgacc agcagaatct ccagattggc gtacgtctgc gcagcaacgg
      661 acgccacgca ttcgtcgagg aatttctcga ccttatatac cgggatgatg atgctgacca
      721 acggctcctg ttccatatct ccagcgtagc gccgccgtgc gaacgcggcg gcgctgcggg
      781 ttcgcggacc gcgaaaccct tagaagtccc agtcgtcgtc atcggtttct tcggctttgc
      841 ccatcacgta ggaggagccg gagccggaga agaagtcgtg gttttcgtcg gcgttcgggc
      901 tcagggcggc caggatcgcg gggttcacat cgcaaatctc agccgggaac aaagccgggt
      961 agccgaggtt catcagcgcc ttgttaccgt tgtaccgcag gaacttctcg acgtcatcgg
     1021 tcaggcccac gcagtcgtac agcgactggg tgtactccac ttcgttgtcg tacagctcgt
     1081 tgagcaggtc gtacgtgtag tcctgcagat ccagacgctc ggcatcgctc agctgggcga
     1141 tgcccttctg gtacttgtag ccgatgtagt agccgtggac ggcctcatcg cggatgatca
     1201 gacgaatcac gtcggcggtg ttcgtcagct tggcgtgcgc ggagaagtac atcggcagat
     1261 agaagccgga gtagaagagg aaggactcga gcagggtgga ggccaccttg cgcttgtacg
     1321 ggttgtcgcc ctcgtaatag tcgagcacga tctccgcctt cttctgcagg aactcattgt
     1381 tctcgctcca gtcgaatgcg gcatcgatct gctcggtgga gcacagggtg gagaagatgg
     1441 acgagtagct tttggcgtgc acggattcca tgaaggcgat gttggtgtac acggcctcct
     1501 cgtgcggggt gagcgcgtcc ggaatcaggg agacggcgcc gaccgtgccc tggatggtgt
     1561 caagcagggt caggccggtg aagacgcgca tggtgagcgt atgctcgtcc tcggtcatct
     1621 tctgccagct ggggatgtcg ttggagacgg ggactttttc gggcagccag aagttgccgg
     1681 tcaggcggtc ccagacctca aggtctttct cgtcttcgag gcggttccag ttgattgcgg
     1741 agacgcggtc gatgagtttc agcggtttgc gcggaatgga aatcggtgcc atggggattc
     1801 ctttgcatta ctcgtcttgg gagttgtcgg cgggcttgtt ttcgaactcc tccggcgcgg
     1861 tcagccggag cagtgctcta tagatggttc gtgacattgt gggttcctta gagtggcctc
     1921 tggttgttcg atgtgtgcgg agaccgccgt ctgaggccga aggcgtacga aggtacgtcg
     1981 agtgccgaag aaaggcggta tacgctccct cgaacgatca gagcatgcag gagacgcagc
     2041 cttggacttc agtgccttcc agggcttgct ggcggatgcg gatgtagtag agggttttga
     2101 tgcccttgcg ccaagcgtag atctgggcct tattgaggtc gcgggtggtg gcggtgtccg
     2161 ggaagaacag cgtcagcgac agtccctgat ccacgtgctg ggtggcctcg gcgtacgtgt
     2221 cgacgatggc cttccagccg atctcgtagg cgtccttgta gtacccgagg ttgtcgttgg
     2281 tcatgtaggc ggccgggtag tacacgcgcc cgatcttgcc ttccttgcga atctcgatct
     2341 tggaggcgat cgggtgaatc gagctcgtgg aatggttgat gtacgagatc gagccggtgg
     2401 gcggcacggc ctgcaggttc tggttgtaga tgccgtcctt cagaatcgcg tcgcgcaggg
     2461 cctcccagtc ggccgcagtc gggatcgcga tgccgtactg ttcgaacagc gcacggaccc
     2521 gttcggtgcg cggagtgagc gaacgacgac catcggtgta cttgtcgaag tagttgccct
     2581 ggccggccgg cttggcgtag tcgctcgtag cgaagctggc gaacgtgcgg ccatgctcca
     2641 cggcgagcgc gtgcgaggca cggtaggcgt ggtaggccac ggtcatgaag tacatgtcgg
     2701 tgaagtcgag gccctcttca gaaccgtact ggatgccttc gcgggccagg aagccgtgca
     2761 ggttcatctg gccgagaccg atggcgtggc cctcctcatt ggcgcggcga atcgaaggca
     2821 ccgcgttgat gctggtgtgc tcggccaccg aggtgagtgc gcggatcgcc gtctccaccg
     2881 tgtggcccaa gccgccatcc atcgtcttag cgatgttgag cgaacccaga ttgcaggaga
     2941 tgtcgcggcc gacatgcgca tacgtgaggt cctcgttgta tgcgctcggc tcctgaacct
     3001 gcaggatttc cgagcacagg ttggacatgg tcacgcgacc ttcgatcggg ttcgcgcggt
     3061 tgaccgtgtc ctcgaacacg atgtacgggt agccggattc gaactgcagt tcggcgatgg
     3121 tggtgaagaa cttacgggcg tcgatgtagg tcttcttgat gcggtcgtcg gccaccatct
     3181 cgtcataatg ctcggtaacg gagatgtcgg cgaacggctt gccgtacacg cgctccacgt
     3241 cgtacgggct gaacagggcc atctgggcct tctgtttggc cagttcgaac gtgatgtccg
     3301 gaatcaccac gccaagagcc agcgacttga tgcggatctt ctcgtccgcg ttctctcgct
     3361 tggtatccag gaagcgcagg atatcagggt ggtgggcgtt gaggtacacc gcgcccgcgc
     3421 cctgacgtgc gccgagctgg ttggcatagg agaaggagtc ctccagcagc ttcatgaccg
     3481 gaatcacgcc gctggactgg ttctcgatgt gcttgatcgg ggcgccgagc tcgcgcaggt
     3541 tgctcagcag cagggccacg ccaccgccgc gcttggagag ctggagggcc gcgttgatgc
     3601 cgcgcgaaat cgactccatg ttgtcttcga tgcgcaccag gaagcagctg accggttcgc
     3661 cacgctgggc cttgccgagg ttcaggaagg tcggggtggc gggctggaag cggccggcaa
     3721 gcatttcgtc ggcgtattcg atggcctgct gctcattgcc ggcggcgagc tccagggcca
     3781 cagcggcgca acgctgcggg aagtcctcaa gatagaggcg accatcgaac gtcttgagcg
     3841 cgtaggacgt gtagaacttg aacgcaccaa ggaaagtgcc gaactcaaag ccggcggatt
     3901 ccacgtgctc gtagatgcgg tcgaggaatt cagcggagta ctggttgaag accgcagggt
     3961 tgtagtactg gttgctgatc aggtattcga gacgctcggc ggtggaggcg aaccgcttgc
     4021 tgttggtggc cacgtgccct gtcacgtatt cgcgttcggc ggccttgtct ttgtcgaact
     4081 ggatcttgcc gtcggcgtca tagaggttga gcatcgcgtt gagcgcgtgg taatcgtgcg
     4141 ccgggtcgta ctgttccgct gcggtgtcgg cagtattgtc gagcggcagg ccggtggcgg
     4201 tgaggtcagt cattggaaat ccttggaata tggcagaagt gtgtttactc agacagttgc
     4261 tcccctcagt caacttcgtt gacagctccc ctcggagagg ggagccaata cctcgagggg
     4321 gtcaagattt actgttcggt gaagaagcgg acgaggccgt ttcgcacggc ggcagtgtct
     4381 tccggggtgc ccatgagttc gtaacggtag aggtagggca ccttgcattt ggcggcgatg
     4441 atgtctccgg cggcacagta ggcctcaccg aaattggtgt tgcctgaggc gatgacgccg
     4501 cgaatccact cgcggttggc tggatcgttg aggaagcgtt tgacctgaat cggcactgct
     4561 ttcttgacca cgccgccgcc ataggtgggc accatgatga cgtacggttc gcggacgttc
     4621 agcgccgggt cggcggcccg caacgggatg cgatagacgt tgatgccttc gtcctgcaaa
     4681 cggcatccgg ccacgaaccg tgcggtgttt tcggaggccg acgagaagta gaccaatgcg
     4741 ccgatcgtct tgccttcggc cgcgaattcg ggctggctca gactcacgcg gacaccgtgg
//
LOCUS       DAWBMW010000115         4783 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_3819_length_4783_cov_2.721235, whole genome shotgun sequence.
ACCESSION   DAWBMW010000115 DAWBMW010000000
VERSION     DAWBMW010000115.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 4783)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4783)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4783
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3819_length_4783_cov_2.721235"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          957 a         1398 c         1463 g          965 t
ORIGIN      
        1 cacatcggcg tcgagcagcg cgcggcggat ttcacggatc gtgccgtcga tatccgcctc
       61 ggaaagcttg cccttgctct tcagatgctt gaacgcattc gagagtctgt ctgtcaaaga
      121 tgaaaaagct gccataatgg gcaacagtct agccgccacg cgggaaattg cggtgttggg
      181 gacgacgctt ttcggggaat gtgccgcgta gtagtcaggg atgttgggca ttcagacggc
      241 aaatgccgtg ccgtccgctc gtggcgaagc caatcgagtg cttccgatac catggaaacc
      301 gtaaagaatc ctgtgttttt tggaatactt acgctgtttt gaaaatcgct ggacaccgct
      361 tatacgcaga ctgccgtgcg ctccgaacca tgacgttcgg gccaacgcag acatgccgtg
      421 caataaggaa ggcccactga tgcccaccca aaaactactc gaacgcaaag ccctgatcgt
      481 tggtatcgtc atcaacgtgc tgcaggtctt agccggcatg gccgtgttct tcatgaccgg
      541 tctcaaggcc atgttcctcg acttctcctt caccgcgatt tccgtcctgt ccggaatggt
      601 ggcggtgtac ctctcgtcgc gcacggtgcg aaccaccgag cgcttcccca atggcatgtt
      661 cgcactggaa cctatctacg cgatctgcaa ggccattttc accatgtcgt tgttggtgta
      721 ttcgctgatt gacgtgtgcc gagtagcgta cgactacttc gtgttcgggt ccggcgaacg
      781 cattgaaacc ggcccggtgg tcatctacga gatcctgacc gtaatcgtgt gcttcagcct
      841 atacacgtat taccggcgct ccaaccgatc catcggcgat tccagcacca tgctcaccgc
      901 cgagtgcaag agcacgttgg tcgacggatc gatgtcgttc ggcatcggcg tagttgccat
      961 cttcctgatg ctgctaccgg ccggcggtcc gcttgacttc ctgcactaca ccggcgattt
     1021 cttcatcacc gtggctttgg tggcattgac catcaaggag ccgttcagcg tgctcaaaga
     1081 ggcgttcgtg gaattggtcg gcggcgtgca tgacgacgat gaaaccaacg cctacgtgga
     1141 ggccgaggca cagcgccatc tgcctgccaa caccgactac gagcagacgc tgatcttcaa
     1201 gaccggtatg aactacaccg ttgacgtgta tctgagcggc atcggcgaaa ccattgatgt
     1261 agctgacctc atcgaatgca agcgctcgct ggaaaaggag ctcagcaagc gcctgcacat
     1321 cgtggacgtg gacttcgtgt ttgactgagt gatgggtttg aagcgactac ttcagactcc
     1381 aagtggaacc ctgcgggccg tcttcgatgg cgatgccggc ctcggtcagg gcgtcgcgaa
     1441 tctgatcggc cttggcgaaa tccttggcct tgcgagcttc ggcacgggcg ttgagctgtt
     1501 cggcgatcag tgcctccagc gctgcatgct cgggcgactc ggccgtgcca tcagcggcac
     1561 cgccggcagc gccggcggac acccacggtt cggccagcgg gtccaggccc aaggtgtcga
     1621 gcatggcacg cacggcgagc agcgcctcac gcacctcggc cttggcggtt tcggaatcgg
     1681 cgcggtcagc cagctgtgaa agcaacgtat tgccggaacg gatggcggtg aagatcgcgg
     1741 cggttgcacc agacacattg acatcgtcat tcatggcggc cacgaaatcg gcgggcaaat
     1801 cgtcggcgga gacggcagtg acttcctcgc gagaaggctg tccacccagc gccacaccgg
     1861 cacgctcgat gaagttcgag acgcggtcgt atgcggcctg cgcctcgacc agcgcctgat
     1921 cggaccattc gagcatcgac cggtactgca cggagcccag ggcgtaacgc accacccaag
     1981 cggaatgctc agccaacacg ctcggcacgg acaggccggt gcccagcgac ttcgacatct
     2041 tctcgccctt ggcggtcacc catgccgaat gcatccagcg cgcagcggag gggtagccgg
     2101 ccgcacgggt ctgagccatt tcgttttcgt ggtgggggaa gcgcagatcc aagccgccgc
     2161 cgtgaatgtc gaagccatcg cccaggtaac ggtgggacat ggcggagcat tcgatgtgcc
     2221 agccagggcg gcccacgcca aacggcgtcg accaacgggc gtcctcggga tcggtgtcct
     2281 tcggagcctt ccacagggcg aaatcacgcg gatcatgctt gtcgggggag gcatcggccg
     2341 ggtcgaccgg attgtacttg tcggcaccgg tggcgtccac ggacgggccc atgcggtcgg
     2401 ccacggcggc ggcctcatcg acttcggagg tctgcttctg gtgagtgagt tcgccgtaat
     2461 gcggccagga tgccacatcg aagtacacgt tgccggtggg cttgccgtcc gcatccgtga
     2521 ccacatagcc gtgtccgttg tcgagaatgc gctgaatcag gtcgatcatg tccgacatgt
     2581 ggccggtggc gcgaggttcc acggtgggcg gcagtacgcc gagtgtgttg tatgcctcgg
     2641 tgaattcgcg ctcgtagtaa taggcgcggg cccaccaacg ctggcctgcg gccgcggcct
     2701 taacgagaat cttgtcgtcg atatcggtca cattacgcac aaaagtgact ttgtagccga
     2761 gcttcaaaaa ccagcggcgc acgatgtcga acgccacggc ggcacggata tggccaatat
     2821 gcggtgacga ctgcacagtc gcgccacaca cataaatgcc gacttcgccc ggcttgagcg
     2881 gcacgaaatg gctgacctga tgcgaggcgg tgtcatacag attcaggcct tctgcggcct
     2941 tggcaacatg cactggcgtc aggcttgaag gcatgacgga aatgttgaaa tcctgcggtt
     3001 cttggctgtt tcccataaca ctcaagcgta gggtccgaac cagacatgct gaatcgttac
     3061 tcgaagagtt tgatgaacgc ttcacaggtg gggcccgaac gaggtgacag acggatgaga
     3121 agcgggacaa cagtctcgcg agaacacgtc tttgaccgct gcggaaactg cgagattcat
     3181 ggatttgccc tgctcgttac gcaaacgggg aacgctcgtg ccacaataga agtcatggcc
     3241 acaccacaag tgctcatttt gcaacatgtc ccatgggaac gcccgggacg catattgtcg
     3301 aacctcgagg acatcggtct tgagacggtg acgatgaata tcgtcgacaa aaagaaaccg
     3361 gacttgcctg atttcggcga actggctggc gtggtaatca tgggtgggcc tatgggcgcg
     3421 cttgattacg ataagtatcc ggggctgaag gcagaagcca agttggcgcg tgccgccgtg
     3481 gcttcaggca agccgattct cggtgtatgc ctggggcatc agattatcgc cactgcgctt
     3541 ggtgcgcagc tgcgcaaggg cgatgcgccg gagattgggt tcgcgccgat caagcgtgtg
     3601 gacaagcatg acttcttctc catgtgggat aagcagctga cagtgctgca ctggcataac
     3661 gacgtggtgg gcctgcccgc ggaaggccag ctgctgtcgc gttcgtcttc caccaaggtg
     3721 caggcattcc gactgggatc cgcgctgggc atgcagttcc acctggaagt gtgcggctcg
     3781 ctgctggacg aatggctgga tgagccgagc atggtcaagg atctcaaggc cgcgggtggt
     3841 tccaagtccc aattgcgcga gcagttcgcc caatacgacc agctactgca accgctcgcc
     3901 gagcaagtgt tctccggttt tgcggcccgc tgcaactcct acgctcagac gctgaacaaa
     3961 taacgtttgc gggccgctgc tgggcaagcg tggcgctaca ttggtgagtc atgccgactt
     4021 atgacattgg actggaacac gtttcgctcg ctttcgctac caaaaccatc ttcactgatg
     4081 taacgcaggg tgtttttgaa ggcgaccgca tcggcatcgt cggcaagaac ggcgacggca
     4141 aatccacgtt gctgcacctg ttccgcggtg ctcaggagcc ggattccggc cgcgtgacaa
     4201 agcgtggcgg actcacgttc ggcatgcttg accagcgtga tccccttgat gacaacgcca
     4261 ccattcgcga agccgcactt gaggggcgcg ccgactacga atgggcgtcc gataacactt
     4321 cccgtgaaat cgtcgaggca ttgctcggcg gcatgagcct ggatgccaag gtcggctcct
     4381 tgtccggcgg ccagcgtcgc cgcgccgatt tggcccgact gctgctcaag gattgggata
     4441 ttcttgcact tgacgagccc accaaccatc tcgacgtggt cactatccac tggcttgccg
     4501 aacatttgaa gaaccgctgg tccaaaggcc agggcgctct gttgctcgtg acccacgatc
     4561 gctggttctt ggacgaggtc tgcgaatcga tgtgggaagt ccacgatggc gtgatcgagc
     4621 cgttcgaggg cggctattcc gcatatatgc tgcagcgcgt ggaacgcgat cgccaagccg
     4681 atgtgcgcga aaccaagcgc cgcaatctgg cccgcaagga gctcgcatgg ctttcccgcg
     4741 gtgctcgtgc ccgctccacc aagcagaagt tccacgtcaa ggc
//
LOCUS       DAWBMW010000116         4782 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_3821_length_4782_cov_2.616670, whole genome shotgun sequence.
ACCESSION   DAWBMW010000116 DAWBMW010000000
VERSION     DAWBMW010000116.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 4782)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4782)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4782
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3821_length_4782_cov_2.616670"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1012 a         1389 c         1462 g          919 t
ORIGIN      
        1 ggccaaactc gatcaattgg tcgcgaacct gcccgacaag gccagcgaca tgggcatggt
       61 cccgcagcag ggatcagtgt ccatcgactt aagcggatac cagtccaaca aaggcggaac
      121 ccagtgaaca ccattcgtcg catcgtagag ttcgccaagg tcaagacctt cgcgttcaaa
      181 tgcatcgcca tcggcgccgc attggcgttg gtggcgtcgg catgcgtgat tcagctggcc
      241 tccacccagc tcatcggcgg ccgtcaaacc gcacaagcgg ccacggctaa ccgcaccctg
      301 accgtgacgt tgagcgccaa acgtgggcgt atcctcgacg ccaacggctc cgtgctcgct
      361 caaagcgtgg agcgttatac tatcgtcgcc aatccggagg ctgcacagga gttcgagccg
      421 attacttgca atgacaacac ccgtgattac tgccacgaaa ttgacggcaa acccgtgggc
      481 gctaccggag cggcggccgt gggccgactg ctggccaaag tcctggatat gaacgccatg
      541 gaactgggcg ctgatctgtc cggtaccggg cagtatgtga ttctgaagaa agatgtgact
      601 ccgcaggtca agcgcagcat cgaggcgttg cacctgggcg gtattgtctg gggtgagctg
      661 agcagtgaac gactgtatgc ggatggcgat ctcatgggcg cgctgcttgg cggcgtgaac
      721 gacaacggcg atggcgtcgc cggcatcgag caggtcgaga acaagacgct taccggaacc
      781 gatggccatg aaacctacca gcgtggtaat ggcggcgaag agattcccgg caccatgacc
      841 gaatcggtgg cggccaaaaa tggcgacgat gtcaccctga ccattgaccg tgatgtgcaa
      901 tggtatgtca aaaaggtctt gaaggaggcc gaatccaagt atggtgcagc gtgggcgatc
      961 gccgtggtgc aggatgtcca gagcggggag attctcgcac tggccgatag cgatgaagta
     1021 caggctggta ccgatgcggc gaagatgagc ccgtctcggg ccgtgtccga aaccttcgag
     1081 cctggttcgg tgggcaaggt catctccatg tccggctatc tgcagatggg actgcataag
     1141 atgagcgatc aattgtcggt cccggaccac atcacacagg agggccagac ctacaaggac
     1201 tcattcgacc atggcactga gcgctggacg ctcgccggca ttctgcagaa ctcatcgaac
     1261 gtcggcatga ttatggccgg ggagaactat ccggatgagc aacgctacga gtacctgacc
     1321 aaattcggca tcggacaacc gaccggactg aacctgcccg gtgaatcgag cggtctgctc
     1381 accaaccctt cggcgtggga tctgcgtacg cgcaacacca tcctgtttgg tcagggctac
     1441 accgtcaacg ccctgcagct caacaacgtg gtggccacca tcgcgaacaa gggtgtcaag
     1501 cagcagcagt cgatcatcaa atcaaccacc gatgccaatg gcaagaccac tgagaccaag
     1561 aagggcgaag ccacccgcgt ggtggatgaa aaggtcgcat cggacatgat gaatgcgatg
     1621 gaatcagtgg cggaaggcta caacaagttc gtcaaggtcg atggttaccg catggccgcc
     1681 aagtccggta ccgcagaggt gcagggctcg gatggtacgc tctcgtccat tatctccgat
     1741 tattccgtgg tgattcccgc ggacaacccg cgctatgcgg tcaccgtggt gatgaaggat
     1801 ccgaatggtg tgttcggcgg gctgacggcc ggccctgttt ccgcacagat ttgtgagttc
     1861 cttatgcaga aatacgaggt gcctgtctcc agtccacgca aaaatgctat tccagtcacg
     1921 tggtaaggat caggggagaa aggcgatagc ccatgagtgc agtgagcgaa tccatcgccc
     1981 agcgggtgac cctgggaatg ctggtcgaac ggtacggttt tgaagtcgat ccacccttcg
     2041 ccaccaatgt caccattacc tcactcagcg atgccgtgga caccgtgatt cccggctcgc
     2101 tgttcatctg cacgcatgag caggaacccg atgtactgca tgccgcacag gccggtgcct
     2161 atgcggcgtt gctgccacgc gcgtccaaag ggcagatcgc caatgccgat ataccgttgc
     2221 tctacggcga tttcgacgat cgcgtactcg gcgatctggc cagcggtctg gccggcggac
     2281 cgtccaatgc catggcggtg ttcgcggtga ccggtgcgga tgagcaggcc gtcgacgcag
     2341 gcgtgtccca gctctccgaa ttcctgcaca tgctgggcaa tccggtggcg gtaatcaccg
     2401 catccgggtc cacgtcgatg acacgtacca tgaacctgaa ctatcctctg ggcatcctcg
     2461 atatgcagcg cgcgctgtcc gtgtgcgccg aagacggtgt ggcggccgtg atcatcgcca
     2521 tggatgaccg cacgttggcc gagcatgcgt tggaatcggt gaacgtggat gtactgggca
     2581 cggaggatgt caacgcaagc gcaagcttga acgaattgaa gacacgctac gcattcgttg
     2641 cggaacatga catgtccatg acctcgtcga cacccgaatc ggatgaaatg gctgcggatt
     2701 caccggcaat gtatgaccgc gtccgactcg ggcacatgtc cttggccatc gccatggtgc
     2761 tggccgccgg cgtgaggaaa aacaacatac gcagtgccct gcatgtggcc aataatctca
     2821 attaagtccg tggatacggg acgaagcgta atcgtaaagg agtttgaaac accatggttc
     2881 cgatgagcgt ggaggagatc gccaaggccg tcgatggtcg attgatcgga gacgccggtt
     2941 ccgagtctcg agccaccaat acggtcagcg actcgcggca ggccggcccc ggatcggtgt
     3001 ttgtggccat ccgaggcgag cgtgtcgatg ggcatgactt tgtggcgaaa accgctggac
     3061 agggagccac ggtggccatc gtcgaccatg aggtgaagtc cgatggcctt ccgcagatcg
     3121 tagtgaaaga cacggtcgac gcccttggcg aactcgcccg atacaacatc gttcgccgcc
     3181 gcgagctgga cggcgacttc gacatcatcg gattgaccgg ttcggtgggc aagaccacca
     3241 ccaaggatct gctctcctcc ctgcttgcca ccgtaggtcc gaccgtggct ccggtgggat
     3301 cgttcaacaa tgagatcggc ctgccgctca ctgcgttgcg ggtcgatgag cacacccggt
     3361 tcttcgtggc tgagatgggt gccagccata tcggcgaaat cgcgcgactg acccgtatcg
     3421 caccgccgaa cacggcaatc gtattgaagg tcggcgtcgc tcaccttgga gaattcggct
     3481 cccgcgaacg catcgcacag gcgaaaagcg agatcgtgca gggattgctt cccggaggca
     3541 ccgcggtact gaacgccgat gacgaacacg tggttcccat ggccggcctg gccaacggcg
     3601 acgtgctgtg gttcggcttg ggatcgtcgc aggagcccga ggtgcgggcc attgatgtca
     3661 ctgccgaccg gtccgatcat gctgaattca cgttggtcga tgcggacggc aaccatacgc
     3721 cggtgcatct gggcattcct ggacgtcaca atgtgatgaa cgcgttggcc gccgcaaccg
     3781 tggcgatgcg ttacggtatg gcacccgaaa cggtggcacg gattctggcc tcgcagcaca
     3841 ccatcagccc tcatcgcatg gcattgtcta cggtgaatcg cgaggggact tccttcacgc
     3901 tgatcgatga ttccttcaac gccaatcccg actccatgaa agccggcctg aacgggttga
     3961 aggcttggaa ctcaaatgac ggcaaggagc cgttccgtgt ggcgctgctg ggtgccatgc
     4021 tggaactcgg cgtcgatgaa acggcattgc acacctcgat cggcacgtat gcggccgaat
     4081 tgggcatcga cgcgatcatc gcggtcggtt ccgcccaaga ccaacatctc gatgcactgg
     4141 cacaggctct ggcggacggt gcccgtcaga gccaattcgc gtcggtggat tgcgtacacg
     4201 acatcgatgc ggccgaacag ctggttatcg acttggctcg caaccatccc gacgctgtgg
     4261 tactgctcaa gggatcgcac gcgtcaggcc tgagcgcttt ggctgaacgc tgggcaaaga
     4321 actgacctga taaccacccc aagaaaccga caagaaagag aagctgcacg tgattgctct
     4381 gatcattgga atgctggtgt cgctcatcgt caccttagtc ggcaccccgc tgctgattcg
     4441 gctggtccac aagctgcact atggccagta catccgccag gatgggccgc agtcgcatct
     4501 ggtcaagcgc ggcaccccca cgttgggcgg cgtggtcatc aacttcgcga ttctgctcgg
     4561 ttgggcatcc tccgcactgt accgatacct gcgttcgggc gacgtgccct cctggtccgc
     4621 ggcgctcgta ctgttcgcca tgctgtcgat gggtctgttg ggctttatcg acgacttcgc
     4681 caaggtgcgt aagaagcaga acgaaggcct atcggtgggc ggcaagttca tcggccagtt
     4741 catcttcgcc accatctacg cggtacttgc gctgctgatt cc
//
LOCUS       DAWBMW010000117         4716 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_3871_length_4716_cov_2.122291, whole genome shotgun sequence.
ACCESSION   DAWBMW010000117 DAWBMW010000000
VERSION     DAWBMW010000117.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 4716)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4716)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4716
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3871_length_4716_cov_2.122291"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    756..765
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          980 a         1298 c         1426 g         1002 t
ORIGIN      
        1 tcatcacccc gatcggtcgc ttcaagtccg agaagttcga tgtcaccgtc gccgacggcg
       61 gctccggcaa gctcacctgc gaactgcgcg acgagctgct cggcatccag ctcggcgaag
      121 tcgaggaccc gcacaactgg atgtggaaag tgtgctgaaa cgcccgctga ttagacattg
      181 agaccccttg cgaacgccaa tgtttgcaag gggtctcatc gtatctcggg taagatttcc
      241 ggcgtctagg aatgtcattc tccaacattc tccaacaaat cttcgggttg ggctgcggaa
      301 cggtttcggc gtggcgaaaa tcgacggtga ttgccgatgg gaaaaaatcc gcgaagcgct
      361 ctagttatat atctctatca ataacttgtt tattacctat ataaggttag tgctgctgaa
      421 ataccatcag ataggctgta gcaataatgc aaagtattgc tgctgagata ttgcgtgcta
      481 tggtagtaga cataaaagaa aacgcctccc ggtgctggaa caccgagagg cacaaaggaa
      541 aacgatgcaa cattttccgc gtccagatta ccgctaggcg tggaggaaag atggagacct
      601 tgggataccg taacgcacaa aaagtcatgc gcctatcagc cgaaggaaaa ttcctcacca
      661 tgtttcgagg taaccccacg cccaacatga cggcatacgc catgatgctc actatggccg
      721 agatctccta tgattggccg cccaccgagg aaaacnnnnn nnnnnaatcc tcagccatga
      781 ggccctacag cgcgaagata acggacgact gattgcctca aggcgacagt cggcgataca
      841 gcgtataagc cagactggca tgttcttaca gtcacaaggg cttatcaaaa aaatacgcaa
      901 ggaggacgtg cgtagggaga ttcccgcagc atggctgctg cttatcggtg acgatgaaga
      961 gaaccgtgag gttgaagcct atgcgaggga atgcctgggc ctgagctcct gttaacgttg
     1021 aacatatagg atatcaggtc aatactggga aggagaagtc atgaccctac tgtggcagga
     1081 tgcgcgccgc aaagcgcaag aaacccttga caattactgg gaccactcct atccggtcaa
     1141 gatcgtgagc atatgcaagg ccatgggtgt cacgccctac accggtgagc ttcccgaagg
     1201 tgtcagcggc atgatagtca aggaacacag cagcgagccc cgagcctaca cggaacgcac
     1261 cgaaccacag acaagacgca ggttcacgct cgcacacgaa ctggggcatt tcgtggaacg
     1321 agtcaccata gcgcaggaca acgatttcgc tttcatggac aaacgttccg acgattacga
     1381 catccacgaa ttctacgcgg acgagttcgc cggtgcgctg ctcatgcccg aacacgattt
     1441 catccagaaa gtcaagaacg atgggatgat cgcggccgca gcatatttcg gcgtctcgct
     1501 ggccgcggtt cgaaaacgca tggaacggtt gcgcaagcac ggggccgaca tctgacatgg
     1561 cggattatga cgacaccggt ttcgacgctg tggaatcgcc ggacgcggac ttagacgcgg
     1621 tatccggcat atcctccacg gatgcgagca agaccgtggc tgcggcgaaa gtggttgagg
     1681 aaaacgatcc gttggattcg cctcaagcca agcaatcgaa tgatgtcgct gatgcggacc
     1741 agttcgtgcg tatggcgcga tggcgtccgt tcatgcacct gttccacgac tgtgcgacct
     1801 cgacgcatct ggataagaat aggggtgaaa gaatgcgggt gttcgcgcgg gagcatccat
     1861 tgttccttag agcatgtctg ctaggagact acgttttcgt cgcggtctgc gctctgatag
     1921 tcgcgcttgc attggcgtat gccttggccc gtgtggtcgg tgttcctttg ccatgggctt
     1981 gaacttggtt ttgaatacgc gaaaaccgcc cctccgtcca gcgttatgcc ggatggaggg
     2041 gcggtgttta ttcgctgcgg ctacttgccg gccatgcgta cggggttgta tgcgactccg
     2101 aatcctgcgg cgatgatgcc tgcggcggta ctgatgaaac cgccgatttc gggggagccg
     2161 aagctcatga acccgagtcc gatgaccgag gcgacgagcg tgaccacgta gatgacggtg
     2221 cgcaccgtgt cattgaacac gggagtgtac ggcgtggccg tatggtcggg aatattgggc
     2281 gtgccggttt ccgtgatttc ttcgagttgg gtgtccggcg tgttgttccg atcaaaaaaa
     2341 tagtggtgat gccgccatcg ggagtaatgg cggcatcggt tggttttagc ggcaggtcac
     2401 cacgtcaccg gggtagtaga cgttgatgtt gccggagggt acggtgcatt ggctgacgct
     2461 gtagccgtgc gcggtggcga aatcccacac ggtgtcgccg tactggaggg tcttggaaac
     2521 cccgttggac ggcgcggccg tggtggaacc gccgccgtag gttacgacgt cgcccacgta
     2581 gtagcggttg atgtcaccgc tgggcgtatg ccatgcggac aggggccatg catccccaga
     2641 tggtttctcc ccattgcatg acgtggctga tgccacccgt gttgggagtg ggctgggggt
     2701 cgctcggctg tgcgggcgcg gccggtgtgg ccggtggcgt ggagccgccg gccgggttgg
     2761 cgtacagatc ccactgccat gcctcgccac ggaagatgtt gaggtcgatg ggactccacg
     2821 tgttgacgac accggtgccg ctgtattggc gcatggcctc gccgtacgcg ccgatcatcc
     2881 acggattggc ctgatagccg gtcgggctca tgttcgcgta ttgggcgatc cacaaaccgt
     2941 atcggtcgcg gatgtcttgc gggatggtgc cggcgaccgg gccggtgtac agcaatgggc
     3001 gcacaccacc cgaaagccgt tcgcactcac tcatgaatcg acgcacccaa tcccagtctc
     3061 cccatgcggg gttgtcgtcc atctcccaat ccaacgccac gatgccgtga cgccaatagt
     3121 tcgacgtatt cgtgtagaag aaccgggcct cggcctccgg gttgcctccc atggcgtagt
     3181 gatacaggcc gaatttcttg ccggatgcct gcgcctgggc gatcatgcgg ttggcgtccg
     3241 tgttgacgcc ggacacgaga cagttgttat acacctgccc cgtgccccat gtggtgccga
     3301 cgacaacgaa gtcggcctgc gtgttggcga tgtcgatgcc gcactgccag ttggacacgt
     3361 cgatgccctg catgtcggcc atcgccgccg gggcgaacgc catggagacc gcggcgacga
     3421 gcgcggacag cgtcacgccc acgcgccggt gcaggcgtcc gtgtttcggc ttgcctttgt
     3481 tgaggattcc cacatcctct ccttcccgcc ccgagtcaag gggcaataga aaagccatcc
     3541 cggaatggga tggctttgaa aacctgtgtg aaaatcaatg cctgtgcgcg ccatgattga
     3601 atatgatgat gagcgcgagc agtagcaggt atatgccgcc tgcgatcatg agatgcgtca
     3661 ttgcctgtcc tccaagtatt tttcggcggc cgagatgatc cagcattgcg cgtccaattt
     3721 ctcaagcttc gtcaattcgt atctgacggc ctctgaatgg tcgtgcgact ggtcgccgta
     3781 gatcaggctg atgatcgtgt ttttgatcgt gtcgcggcag agttcgtcca accgtccatc
     3841 gaatttctca gtacgctcgc ctagctgccg tgtttttgcg aaatgctgcg agagcggcga
     3901 atcgtagggc aggcgttccg gccgcacgtg cgaatacaat ccggtcgcca gcgcgtccaa
     3961 agcgcccggc catatcctga gcagcagggt gatgagggcg cacgcgccac ccactccccc
     4021 gaacccggct agaaaatttt gcaacacatt acatctcctt tatggaaaag ccccgcacat
     4081 ggcggggctt tgatcttggt taatacgggt ggtcagaggc ggcgaacacg agcggcaatc
     4141 cgacatgcca tgaaaatcat tcgctccatg ccggaatctc gccctcaatc ctcggccctg
     4201 aaagcgtgtt gacctattcg cggatcttgt gcgcggcgac attcattgcc ttggcgttag
     4261 ggtggacctt gtcgtgcatg tacccatttt tttcgccgtc cggcatccag tcgaaccagt
     4321 cgcgggcgac gttgaaaccc ttgccctcga acagtgtgta cgtctgacgg aggacgttac
     4381 tgttggatgg cctgtttgat gtgccggccg aggatctggc cgagtttgac ggctccctcc
     4441 gcggcggggt gaatcatgtc ggagacggtc aacccactgc cgagcgccca tacttcgtcg
     4501 gtcgcgtcga tgaccggcac gcccagcgtc gtctcggtga acaggattcg ttccgaggat
     4561 tgcagggccg tgaagttgtt ggtcatgatg gtgccggccg gcaggcccgt catgaccggc
     4621 ttggcctcag tcgcagcgga cctgaacatg cactggagcg cgtagagaat acgagcctgc
     4681 gggtacttgg ccttcagctc gtcgaacttc gccttg
//
LOCUS       DAWBMW010000118         4709 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_3877_length_4709_cov_6.877525, whole genome shotgun sequence.
ACCESSION   DAWBMW010000118 DAWBMW010000000
VERSION     DAWBMW010000118.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 4709)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4709)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4709
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3877_length_4709_cov_6.877525"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          950 a         1455 c         1459 g          845 t
ORIGIN      
        1 gcggcggtgt agatgtcctc cagcagacgc ggggccaccg gccagttgcc gatgccctcg
       61 ggggtcttgc tcggctcctc accctggtac cagacattcc attcatcgaa cgagatgtac
      121 acgtcgtgcg cgctcttcag atgggccttg gtggcgtcga tggtggcggc cacgtccttg
      181 atgaagccgt ccatatccac gccggaggcc aggaagctgg ccatgtcgcg cgtgccgtcc
      241 tccttgaaga acggctggta gtaggcgtgg caggagacga agttcacgtt ctcgtaggtc
      301 ttctcaagaa cggtctgctc ccacttgccg aaggtcggca tgccatgggc ggaggagccg
      361 cacaccacca gttccacgtc cggatcgatc tggcgcatgc cggcggccac ggaggcggcg
      421 agcatgccgt agtcctcggc ggacttgtgg ccgagctgcc acgggccgtc catctcgttg
      481 cccaggcacc acatgcggat atcgaacggc ttgtcggcgc cgttggcgcg gcgacgttcc
      541 gacagctcgg tgccgccggg cacgttcgcg tactccagca ggtccagggc ctcctgcagg
      601 ccgcgcgtgc ccaggttgac ggcctccatc agctcgttgc cgccggtggc ctcaagccat
      661 tcggccatct cgtgcaggcc gaattcgttg gtttcggtgg aatgccaggc cagatcgagt
      721 cgacggggac gttcctgctt ggggccgatg ccgtcctccc agcggtagcc ggacacgaag
      781 ttgccgcccg ggtagcggat ggtggtggcg ccgagttcct tgaccaggtc gatgacgtcc
      841 ttgcggaagc cgtgctcgtc ggcggtcggg tggtcgggct cgtagatgcc ggtgtacacg
      901 caacggccaa ggtgctcgac gaacgaaccg aacagacgat tgttgaccgg agcgacctca
      961 aaatcgtcat cgacgacgag acgtgcggtc agatggtcct gctggggcat gggattcctt
     1021 tctacatatc tgcatgcact ctgcatacgt gacacgtatc catacaacga tgtaccgcgg
     1081 aggcgcgggc gaccggggac atccgtgtga cagccaccag tgtagtacaa ccttgtactg
     1141 actgccggaa ggcgtggcgc agagacggct gagacctttt ggcgcaccac tgcagcaaaa
     1201 aagatcccaa gaagtcgaat tacagtccca tccgacgcat agccaccgtg aacatacggc
     1261 aaaaggcatc aaccacgtca cccgtccacc cgctacattc acaagcagta ccgcccactc
     1321 tgcatcattc gcatatgcaa aggccgcgaa ccgtacgatg acggttcgcg gccccctgac
     1381 atgccattag gccacccggc tcgcaccggc tgttcggcct gccggctcag ccctcgcgct
     1441 tgggcgcctg gctggccacg cgggtcaggc gcggcgggcg gcgcgtggac acgaacagcg
     1501 gctgcacctc gaccagcggg ttgtacggcg ccaggccgaa ccacttgacc ggggaaccgg
     1561 ccacgcggcg gatcgcgtac ttgatctgca gcgagagggc gccacggccg gagaccttcg
     1621 actcgggcat cagcgccttg tggatcacca cgtagcggat ggtgccgatg ccgggatcgg
     1681 cgtcaagctt cgggtagatc gactgctggt tcggcagctc gccggtcttc tccagatcgt
     1741 gcatgatctg atgcaggtag gccgggatgg actgcgagac cttgaagccg aggcggatgc
     1801 gcacacggaa caggtagtcg gtaccgaagc tctcgaccga atactcacgg gtgaacggct
     1861 cgtcggtggt ctcgacggcc accgcccacc atgcgcgggc gcgcttgggg tggtcggcga
     1921 agatcgagaa gaagatgtcc gtatcgagac gcttcaggtc cggatcgctg gtcaggtaga
     1981 cgatgttgtc cgcgaaccgg tggatgcggc tatcgccgtg cagcttgtcc agcgcgggga
     2041 ggaagtcctt cggcatcatg tgccgacgct gcgagcgctc cagcttggtg ccctcgttcc
     2101 acgtgtacat gatcatgagg atcgccagcg tgagcagcag cgtgaaccag ccgccgtgca
     2161 ggaacttggc catcgaggcg gcgaagaaca ggacctggat cgccaggaac acgatggtga
     2221 acacaacggc gccgaacttg ttgctgcggt gccacaggta gatgcccagc aggatcgtgg
     2281 tggtgatcat cgtgatggtc agtgccaagc cgtaggccgc cgagatatgt tccgaatcgc
     2341 ggaacagcag cagcacggcc agcgtggcca cgcacagcac cacgttgacg accggaatgt
     2401 acagctggcc gcgggtacgg gcagggtaac agacctgcag atgcggcatc cagttgaggc
     2461 cggtggcctc ggagaccatg gtgaacgcgc ccgtaatcag cgcctgggag gcgatgatgc
     2521 cggcggtgac cgacagcacg acggccacat agcgcacgtt cggatccatc atctggaaga
     2581 acgggttgat cccctcggcg tcggccagtt cggggttgtt ctggttgcgc agcatccacg
     2641 cgccctggcc gaagtagttg agcaccagcg ccaccttgat gaacggccag gtgaagtaga
     2701 tgttgccgcg gcccacgtgg cccatatccg agtacagcgc ctcggcgccg gtggtcgaaa
     2761 ggaacacggt gcccatcagc gccagaccgg tggcgttatt cggactgaac aggaacttga
     2821 tgccgtagta cgggttgagt gcggcgagca cggaccagtc gttgccgatg gctaccacgc
     2881 cgacgatcgc caggaacgcg aaccagacca tcaccaccga gccgaagacc ttgccgatcg
     2941 attcggtgcc gcgcgactgc acggcgaaca ggatcacgat gatcaccgcg gtgatcatca
     3001 tcgtcagatc cttgttctcg gtgaacagat gctccaacgc cggcagcgtc ttcaggcctt
     3061 cgacggcgga cgagatcgac acggccggcg tcagcaccga gtccgccagg aaggccgcgc
     3121 cgccgagcat ggccggtatg acgagccatg cgccgtattt gcgaatcaac gagtacagag
     3181 caaagatgat tctgcagaaa cggacatcca aacatcccaa ttcacatcac acatgaccga
     3241 ttctccagaa tcggacacac agaacacatt tttcggaact cagacgtccg attcggcaga
     3301 atcggacaca tgtgcacaag tcgaatgatg agcacgacta tcgacgggcg ttcgccggtt
     3361 gtggacgccg ctgaaatcga tgcgatccac accgggcaca cgcatgaaga cgcacagtat
     3421 caacgcgtgg tatcgcctgg caccagcgtc atgggaataa gcacgttcgg ctcgaacggc
     3481 ttggggatgt tcttgcgcga ggcgttggcc gcagccgcct cttcattggc gatcgcctgc
     3541 accatatggt cggccgccga ctgcgcgatc gccgagaaat cctgtgcgac cgcggtcagc
     3601 ttcataccgg ccaaatcggt caaatacgta ccatcatagg cgacgacctg cagctcgtcg
     3661 ggaatggaaa ttccgcggct caacgcctca cgcacgcaga acgccgcgcc aatatcggag
     3721 ctgaccacgg catcgacgcc gtcggtcgcc accccatcaa gcgcacggct gaccgcacga
     3781 tgatagccgg cgaaatccat cacctcaccg gcctcgatat actcatactt cacgcccgcg
     3841 gaggtcaatt cctgttccaa cgtgaggtag tagcgcaccg tcgggaacgt ggtctttccc
     3901 aaatcgaacg acccctcctc gacttcgccg cggaccgcca gatcgaagaa ctgatcgcgc
     3961 ggaccgccaa tcatcaccac atggcgcgcg ccattgcgaa tcagcatctg cgcaatcagt
     4021 cgcccaccct gctcgtggtc agatccgatc gacgaaatac catttccaag cacgcgatcg
     4081 aatgcgacga tcgggcggtg gatggacgtc caataatcgc cgggatgcga ggtgtgagcg
     4141 cacatgacga tgccgtccat catgtgcctg cgcagcatat cgacgtattg gatttcgccg
     4201 tccgtctcgt cggtggtgga gcacagcatg gtgcgcagcc cctgcgcggc gagcgcatgc
     4261 tgcaggtgcg cggtaaacgt tgcaaagaac gggtgacgaa tggtgggcac gatgacgccg
     4321 accagattcg tgcgatcgtg gtacaggttg cgggcaagct cgttcggcac gtaattgagg
     4381 gcctgcatgg ccttacgcac gcggtcgcgc atgtcgttgg atacataccc gttgccgttc
     4441 accaccagcg agacggtgct cagagacacc ccggccttct tcgccacatc gcgcatgccg
     4501 accatgatcg caccttcccc gttatcactg ttctatcgtt gtatgctcac gtgcttacca
     4561 cgaaatatta cattcattta cattctctcg aacctgttcg acagtccggc aacgaaatac
     4621 agcttcttag tgtcgactcc tgcggaggcg aaggccttca gcagcggctt ggtctggtcg
     4681 aatgcaatca ccagcgtcgc atccgggct
//
LOCUS       DAWBMW010000119         4689 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_3892_length_4689_cov_2.194648, whole genome shotgun sequence.
ACCESSION   DAWBMW010000119 DAWBMW010000000
VERSION     DAWBMW010000119.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 4689)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4689)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4689
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3892_length_4689_cov_2.194648"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1708..1717
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4061..4070
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4334..4421
                     /estimated_length=88
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          898 a         1438 c         1407 g          838 t
ORIGIN      
        1 tggcaggtgg gccacatgag cccggaagaa tatgccggcg cggtggataa ggtggcccac
       61 gccatgaagc tcgccgagtc cggtctcgaa ctcgtggcct gcggttcctc gggtgcctat
      121 atgccgacct tcggcacgtg ggagaagacc gtgctcacca aggcttacga gaatctcgac
      181 ttcgtctcct gccatgccta ctacttcgac cgcggccata aaacccggac cgccgcctcc
      241 atgcaggact tcctggcctc ttccgaagac atgaccaagt tcatcgccac cgtctcggac
      301 gcggccgatc aggcgcgaga agccaacaac ggcaccaaag acatcgccct gtccttcgac
      361 gaatggggcg tatggtattc ggacaagtgg aacgagcagg aagaccagtg gaaggcggag
      421 gccgcgcagg gtttgcacca cgagccatgg cctaagtctc cgcatttgct ggaagacatc
      481 tacaccgcgg ccgacgcggt ggtcgaaggt tccctgatga tcaccctgct caagcactgc
      541 gatcgcgtgc gttccgcctc gcgcgcccag ctggtcaacg tcatcgcccc catcatggcc
      601 gaggaacacg gcccggcatg gcggcagacc acgttctacc cgttcgccga agccgccctt
      661 cacgcgcgcg gccaggccta cgctccggcc atcagctccc ccaccatcca taccgaggca
      721 tatgacgacg tgccggccat cgacgcggta gtcacgtggg atgaacaggc ccgcaccggt
      781 ctgctgcttg ccgtcaaccg cgacgccaac accccgcaca cgctcaccat cgacctttcc
      841 gggctgcccg gcctgcccgg tctcggcacg ctcgcgctcg gcaaggcgca actgttgcat
      901 gaggacgatc cgtaccgcac caacaccgcc gaagcgcccg aagccgtcac gccgcaaccg
      961 ctcgacattg cgatgaacgc caccggcacc tgcacggcaa cgcttcccgc catctcctgg
     1021 atcagcgtgg aattccacgg ctaaaagacg gagagtcacg cccgtaccaa gtacagtcaa
     1081 gtccaatacg accagggccc gtcaatcacc atacgattga cgggccttgt cattcacttc
     1141 ggatatttcg aaccatggat tcatcaggcg cgcgccaacg ccggtgagga ctcgcgcagg
     1201 gagaggctat agggggcctc gatggtttgg gcgggggcac ggctgccgga ttcgacctgt
     1261 tggatgatgg attcgacggc cagtcgggcg atggcttccc tgccggggtt aacggtggaa
     1321 agagacggca ccatgtaggc cgattcctcc acgtcatcga agccgatgac gcgaacatca
     1381 tccggcacgg caacgccgcg ttcgcgcagt tcacgcagca cgccccacgc cagcaagtca
     1441 ttcaaagcga acacggcatc gaattgcagg ccactctcca gcatggtgtc caccgcggcc
     1501 aaaccgtcgg cgctccccca gccttgggtt tcgataagca gactgtcatc acaggggatg
     1561 tcgcgcgcgg ccagcgcctc acgatatccg tgggtgcgga tggagcccga cgagaaatcg
     1621 tgctcggggt ggggcacggc accgactacg gcgatgcggc ggcaaccggc atcaagcagg
     1681 tgataagtcg cggcggcggc cccttcgnnn nnnnnnncgc gattgcgaat caccacgtgc
     1741 ggcgcgttga cgccgaacaa acgttcaccc aacagcacga gcgggtaatc gccatccaaa
     1801 gccgcgatat cgtcaaccgt catcatcaga gggcttaaga tgagcccgtc cgcgctgtgc
     1861 gtgcccatcg atgcgatgct ttccagctcg cgggcacgat tgttggtggt ggattccacg
     1921 atgacgccgt agccatgttg ccgggcctcg gccagaatat cctcggcgag ttcggagaaa
     1981 tacacctgcc gcagatcggg aatgaccagc ttgagcaacc gggtacgccc ggaacgcagg
     2041 tttctggccg acgagttgac tttgtatccg agccggtcga tggcttccgc aatacgcgcg
     2101 gccatcgcct cgctcatata tggctgtttg ttcagaaaat tagacaccga ggctaccgaa
     2161 acaccggcct catgggccac gtccgccatc gtcactctgt ttttgcctcg cacggtgacc
     2221 accctacccg actttgctgg agatacgaaa acgactcccc tgctacgatg agcagcaagg
     2281 gagccgttgg gtaattaggc gctatgcgcg gttttacagc ttgacgacga gtacgccgtt
     2341 gggctggatg gcggccgtgt gctcggactc gttgacctgg gccagcgagg cgaccagcgg
     2401 ttcgccttcc acgtccacga ccgcaacatc gtgggtgcgg ttgaacagga acacgaagtg
     2461 gttctcgcca gtttcgtccg cacgctcgac gcgcagcact tcaccgcgat cgtcctcagc
     2521 cgaagtctcg atgccgagtt cctccagcag cacgggcagg ctcttggcca agccctcacg
     2581 gccgagacgg gcaccaacgt atgcggcctt gccgtcgccg aagtcgttga cggtgatggc
     2641 gggagcgccg tcgaaaccgg cccacttatc tgccttgaag gaggcgacca cgtgagcggt
     2701 atcggccacg gaagtgatca cgtcggcgaa atcgtgcgcc acggttccgt tgtccaagtc
     2761 aaggtggtcc atggcaccgg ggaagtcgtt gcccatcggg gcgaattcct caacgcgcac
     2821 gcccaccacg tcacggatgg aaccggggta gccacccagc cagacgtgat ccttgtcgtc
     2881 caccagaccg gtgtagtagg tcacgaacag cttgccgccg ttcgccacat actcgcgcac
     2941 gcggcgcgtg gtctcttcag acaggatggt caggctcggc agcacgacgg cttcgtactc
     3001 gtcccaaggg ccgcgaaccg gcaccacgtc tgcggtcaga ccgttgtccg ccagcgcgcg
     3061 gaaccacgcg agcggctcgg tccaatggcg cacctcctga gtaggcgtgg cggtgtgttc
     3121 cgtagcccac tgggactcgt aatcgaagac gacggcgacc ttggacttga ccagcttggt
     3181 gctcagcagg ccctcgtcag caagcttgtt gaggtcggca cccagctcgc acacatcgcg
     3241 gaagatctgg gagtcggggc ccgcgtgagg aaccatcgaa gagtgccact tctcggcacc
     3301 ggccttggac tggcgccact ggaagtagca gatggcgtcg gagcccatgg ccaagtgggc
     3361 cagggagtcg cgcaccaatt cgccgggttc gacgcgatag ttgattgggc gccaattgac
     3421 ggcggagctg gagtgctcca tgaggaacca cggattcttg cgggcgatgc cgtcgcacag
     3481 ggaggccgag taagccagct cgtcgaagtg ggcttcgcca ggcgtgaagt agtgatcgtt
     3541 ggacacgaag tccacgtcgt aaccccactt gtcgtaatcg atgccggtac cgccggcgga
     3601 gaccatgaag ttggtggtct gcggcttggg ggcgatctcg agcagggcat cgcgctcggc
     3661 cttgtaaaag tcgagcaggg cgtcggagct gaagcgcttc cagtcaagca gtttgcccgg
     3721 attcatgaag ttgccatcgc cgatgaagcg cggcgggatg atctcggaga aattgttcat
     3781 gcgctgcgcc cagaaggcgg tgccccaggc gtcgttgaca gcgtcgatgg tgccgtactt
     3841 cttctcgcac cacttctgga aggcgcgctc ggcgtcttcg gaatagtcga agcggttgtg
     3901 gcagccgtac tcgttgctca catgccaaga gaccacatag gggttgtcct tgtagtgctc
     3961 ggccatcttg cggcacaggt tgagcgcgta gtcaaggaag accgggctgg tggcgcgcca
     4021 gtgctggcgg gcaccgggct ggcagacgtc gccgcggtag nnnnnnnnnn ggcctgggtc
     4081 atccacatcg gcggggatgc ggtgccggag gcgagatcga cggcgatgcc ggccttgccg
     4141 agcttgtcga tgacgcgatc gagccaatcg aagtcgtaca cgccttcttc gggctcaagc
     4201 ttggcccagg agaagatggc tacggagacg aggttgacgc cggcctgctg cataagctcg
     4261 atgtcttcgt cccagacttc ctccggccac tggtcggggt tgtagtcgcc gccgtaccag
     4321 atgcggggct cgtnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncttgaactc tttacgttcc
     4441 atgatgattc agttccttta ttgaatttta tcgataatct gcgtctacgg gaataaattc
     4501 ctcacgttgc acagacacac aaggaggcac ctgcacaatt ctcaacaatg cgaatatctg
     4561 cataggtgcc tccttctgtt agtggttatg cctttatctc ggctccctcc accagaaggg
     4621 agccaaagta cgctcactcc ttgacggcgc ctgcggcaag gccggactgc cagtacttct
     4681 gcaggcaca
//
LOCUS       DAWBMW010000120         4680 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_3900_length_4680_cov_2.239351, whole genome shotgun sequence.
ACCESSION   DAWBMW010000120 DAWBMW010000000
VERSION     DAWBMW010000120.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 4680)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4680)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4680
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3900_length_4680_cov_2.239351"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2401..2410
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          983 a         1425 c         1317 g          945 t
ORIGIN      
        1 gtacaacggg tgaacggcga ggcgcagcag gcgggcgaat atgcggctga agcacagcaa
       61 tccgtcgggc agggcgactt caagcaggcg cttgagcaga tggacagcgc tggcagtcac
      121 atcgtcgcgg cgcgagacga aacccagggc atgctatggc aggccgccga atacgtgctg
      181 tattacggtt cggacgtgaa ggcggtgcgc ggcatgctgg atgcggccgc cgacgccgcg
      241 accaacgccc tgcccaaaat cgacagtgcc gcgcagggca cgttggtgga cttcaatatc
      301 gaagagctgc tgtccggcat gaacttctcc gacggcactc tttccattcc caaaatcgct
      361 tccatcaagc aggacctgtc cgacgccggt gcggtcttga agcgcgtcaa gacgtcgatt
      421 catgcgctgc cgcaaccccg cacggaacag atcaccgaaa tggtcaacca gggcaagcag
      481 gtagtggacc aaatcgatga gactttcgac accttgaaca ccatcgtcga aaagattccg
      541 ggctgacgtt ggttcgttct tgtcgtatat tggcgtaacc tccgcataag gctagtccat
      601 acaaatttat gtttaaattt gggataggag gtgccaatat gccgacacgc acaccaatcc
      661 cggtcagtcg ggcgatgcgt cagatatccg ccaacctcga tttggcgcgc agacaacagc
      721 gcattcccgt agccttgctg gccgagcggg ctggattatc catgccaacg gtcaccaaaa
      781 tgctgcgtac gggtaatggc agctttgaga acttcctccg cattgctcgt ctgcttggac
      841 tgctggacgg cgtacagcaa gcgacggacc ctcttaacac agatatcggc aaattacgcg
      901 ccgacgaaga cactccaaaa cgtattcgat agtacagcag gaggcgcaac acatcatgga
      961 agcaatcgtc accctgcagt tcaacggcac agacgtcacc gtcgggaaat tattcaccag
     1021 tatcaggcgc ggcatcgaat cggcacattt cacatacgat acggcctata tgcgctcttc
     1081 aaacgcagta tccctctgtc cagagatgcc cctctccccc cggaacgttc ccggccgagc
     1141 ataatgcgat gcatcgcatc tttcaagact gcatgccgga tcgttggggc aggaacctca
     1201 tgcttcgtgc agaacatcaa gatgctcgtt cggagcatcg cacagcacgc acactatttg
     1261 agggagatct cttgctatcc gtcaacgatg agacccgaca aggcgcattg cgcttctgga
     1321 acaacgacgg tgacgcgcta gctcccagtg agaccggcgg tccaagagaa gtgaccattc
     1381 agtcccgcat ccatagcaat gatgagcagc tgctttgaat ccggcattgc gcggcttcgc
     1441 gaagcaaacg cacaatgact tcacattccc gccttgcacc agctggctgc aatctgcgta
     1501 tggtcatgta tctgtaggcc ggcacgaatg cggctgatgg gtgcggcgaa tcgtgacatt
     1561 gcttattacc cattcgcccc ttcagtcgta ccattcgggt cgtcgcagtt ccaggaatgg
     1621 ccgctgtttg cgcgctgctg cggtctgtgt gagatcgatg tcggcgataa tcaactgttc
     1681 ttggtcgtcg gccttgctga cgacgttgcc gtacgggtcc accaccaccg attggccggc
     1741 gaacgtcaca tcgccttcct tgccgacccg gttgcacatg acgatggcca cctggttctg
     1801 catggcctgg acgcggattt tccacaggaa cggcttcgac ggttcggcgt cagtgttcgg
     1861 aaccgatgct cgccgtcggc acgatgacca ggcggcgccg cgcaacgcgc atgtgcggat
     1921 gccttcacgg ggttcactca aatagcgtca tccagtcggg atcgcggtgg gccgcaaaac
     1981 acatggcgat ttgttccgcg gtgtttcttg tttccgaaag ccgcggcaac cggtcttcct
     2041 cgaagtagcg gatttccgtc gtttcgatgt tggccgagaa ccaaccgccg gcgcggctgc
     2101 acaatacgaa gaacttgagc accccgtacg gataggtcag cgcgttgtga ttggcgcagt
     2161 cctgcaccgc gatgagtcgc tcgcccctca catcgagccc agtctcctcc ttgacctcct
     2221 tgacggcgtt ggaccgaatg gactggttct cgtcgaccca gcctccgggc agtgaccatt
     2281 ccccgctgtt ctcatgcgtc atgaggatcc tgccttcttc atcgaagatg gccgcacgag
     2341 tgtcgagttt cggcgtcgga tatccggcgc tgagcagtcg actcacctgt ccggccggcg
     2401 nnnnnnnnnn tacatcagtc cggtctgggc cagcgcctgc aattctcggg cctgttccag
     2461 taacgtgcgg ttcgtggcca tcatcgttca tcctgtcatc gtaaggtctt ggatccgttt
     2521 gcgctcaggc atcgcagcgt agtgcaagca gtatagcact tcgaaccgtt ccggggacga
     2581 gggtctcagc gcatcccccg taacctctcc catgtgccat caacgcttgg cgagctgcac
     2641 gttcttgcgc tgtctgcgat tgcggctggc gaccgacttt tcacgctcgc ggcgcacgtg
     2701 ggcctcctca cgcgcggttc ggccttgcgc tccccgcctc ggatccgggc gccggaaacc
     2761 atcgcagttg gcagagcgtc tggccgaagt tccacaggtt attggtcagc cagtacagca
     2821 gcacgccgaa cggcaaggtg ccgcccgaca ccacgtagat ggccgggaac acaaacgcgg
     2881 tcagcttctg cgtgcggaac tgcggtgaat ccatggcgtc cttcggcgta ttgcgatgga
     2941 tgagcagcca ctgcatcgcg aacaacatca gacacatcaa cgccacggcc acgccgatga
     3001 cgacgtgcga ctgcggcgag acgaccgtgc cgaacgagtc ggcgatgcgc acgccgaata
     3061 tggacgatga ttcgatgtcg cccgccatga cgcggtcgat gccgctcaac ggttccgcat
     3121 tcccggcggc gcttgcgggc agggattgca gcgtgtagaa cagcgagcac agaatcggcg
     3181 cctgaatcag cgaagcaggt atgaactgag cggattggcg ttatgccgct gctgcaaagc
     3241 cataatttca cgttgcatgg cttctttgga acgcggatcc ttacgcgcgg cgtacttgcg
     3301 ttggatacgt tggatttgcg gttggagcac ctgcatacgc tgcgtcgcgc gcatttggga
     3361 gaagaagaga ggcaatagac agatacggat gatgagcacg aggcacataa tcgcgatgcc
     3421 taagctgtgc ggcaccgact ccttctacgg cggcggcatg gtcaccggcc agcccatcgg
     3481 caccatgatc ggctctcgca tgatcaacaa tgcgcaagcc ggcttcatca tcggcgccgt
     3541 gatcatgggc ttccggattc gtggcgcttg acatctgacc tcgcgaggaa tcctcaaagg
     3601 acatggaacg caccaagatg acgctcaagg atctcgctgt ctccttccac ttcccgaagt
     3661 tctccacctc gcgtgacttc tacaaggcgt tcggctgccg agtgtgcatg ttgctcagct
     3721 accagatgat ctccgtgtac caactctaca tcgtcgagga ttacgtgcac cagaccaaga
     3781 ccgaggctgc cggcaccatc gccaccatgt ccatcatcac catggtcgtt tccctgaccg
     3841 cttcgctgat ctccggcccg atctccgaca agatgcacac gcgcaaggta ccagtcatca
     3901 tcgcctcgct gctgttcgcg attggcaccg cgatgccgtg gatcatgccg agcgtcacgg
     3961 gcatgtacct gtatgcgggc atcgccggct tcggctacgg cgtgtgctcg gccatcgacc
     4021 aggcactcaa cgtcgacgtg ttgccgaaca aggaagaggc cggtaagggc ctcggcatcc
     4081 tcaacctggc caccacgctc ggccaagcag tcggtcccac catcacctcc gtggtcaaga
     4141 ccacgaccgg cacctacacg ccggcgttcg ccatctccat cgtcatggcc gtgtgcgctg
     4201 ccgtgttcgt gctgcgtatc cagaaggtga agtagccgct gcgagtgcct atctccgttt
     4261 caaggggctg attttgactt tgcggaacat ctacttccgt ttcaaggggc tgatgccaaa
     4321 gtaaccactg gttaaaaacg gcggaatagc gatagaacaa tgccatttct atctgcgttc
     4381 cgccagttga ccagcccctt gaaacggaag tagacctccg gcaaacgcca aatcagcccc
     4441 ttgaagctga ttttgactca gcatcatgca gcgcagctat atcctcggtg cgggtatact
     4501 attccgggcc gtcacgacga cgcctatgga acgcacgcat ggcaaagaga gaaacatgag
     4561 tcagcccgac accgacacgg cggcacagtc gccgtcctcg caacaggaac cgcagattca
     4621 gacggatggc cgtcgaagcc attcggaaca gatgaatcaa ccagcgcaaa ccagccagtc
//
LOCUS       DAWBMW010000121         4625 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_3955_length_4625_cov_2.724945, whole genome shotgun sequence.
ACCESSION   DAWBMW010000121 DAWBMW010000000
VERSION     DAWBMW010000121.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 4625)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4625)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4625
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3955_length_4625_cov_2.724945"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3923..3932
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          976 a         1436 c         1337 g          866 t
ORIGIN      
        1 atctcgttga gcagcaagct ggtggcctgc accatcgccg tgctgctcat cggcgtgtcg
       61 gtgatttcct tctccatccg cgcgttggtg aacaactaca tgctgcagaa aaccgacacc
      121 cagttaagct cgcaaagcca actggtggtc aacaatatcg atctgctgtc aaaaaatgac
      181 tcatcggggc cgaactcgta ctttctgcag attcaataca ccgacggaac caaagacaaa
      241 gaaggcaatc ctctggtcgt gaccccgctg atgccgcaga tgcaggacgg tatcgtatcg
      301 gtgccgattc tgcctaccta tggcgatacc aatggcatca cgctcggtca ggcattcact
      361 acgcaggcgg tggccaagca gatcattacg gtgcagtccg acagtgcgga cagccagaac
      421 gatccggcca acggaaactc caattcctct gacaccatca ccaaggtact ggccaacccc
      481 actgccaatg ccaaccatgc cgccatcgtc acggcgaggg caccatggcg tattctgccg
      541 gtgaccttcc aacaaaacgg caaggaccgt gccgtggtgt acatcggctt gtcgctggcc
      601 gaccaaatcg acaccgtcaa cacactcacc cgatactgca ttgtggtcgg catcgccgtg
      661 gtgctgctcg gcggctccct gtccacgctg atcatccagc acacgatgac gccgctgaag
      721 cgcatcgaga agaccgccgc gaaaattgca gccggcgacc tgagccaacg tattccttcc
      781 gcgccggaaa acaccgaggt cggctctctg gccgcctcgc tgaattccat gctcacccga
      841 atagaatcca gtttccatga gcaggaggag accaccgaca agatgaaacg gttcgtctcc
      901 gacgccagcc atgagctgcg caccccgttg gccgccatcc atggctatgc ggagctgtat
      961 aagatgcagc gcgatatgcc tggtgccttg gagcgtgcgg acgagtccat cgaacatatc
     1021 gaacggtcca gccagcgcat gactgttctt gttgaggatc tgctgtcttt ggcccgtctc
     1081 gatgaggggc gcggcatcga tatgaccggc acggtgaaac tctcgtcgct ggtcaccgac
     1141 gccgtcgacg atttgcatgc gctcgacccg gaccgtgccg ttcgccgcat gcagatttcc
     1201 ctcgaaccgg cgcgcgattt gaaccatccc gccgaattca gccttgccga aggcgattgg
     1261 cctgaggtcg tactgcccgg tgatgcctct cgactgcgcc aggtggtgac caacatcgtg
     1321 ggcaacatcc accgctacac gcctgccgat tcgcccgctg aagccgcgct cggtgtgatg
     1381 ccggccgcca tcgatccaag acagctcgcc cgcatgcccg ccagcgacgc gtcaatgcgg
     1441 cggttcatcg acgctgccga agtaggtgcc tcgatgcaga ccggctatcg atatgccgta
     1501 ttgcgtttcg tggaccatgg ccccggcgtg ccgcctgaat cgcgctcgaa gattttcgaa
     1561 cgcttctaca ccgcggaccc atcccgtgcc cgcgaaaagg gcggtactgg tttgggcatg
     1621 gccatcgcgc aatccgtggt caaagcacat cacggcttta tctgcgccac cggcaccgat
     1681 gggggaggcc tgaccttcac cgtggtgctg ccgattgagc agatcgccgc tcctgagccc
     1741 aagcagtcca ccggcaaaac caaggacgcc aaacagaaga cttcttggtt cagctctgag
     1801 cgtaagactc aggcgactca gcccaaagcg tgaggtcaag ccctgtgagg taaactgtac
     1861 cttcggttta ctatttccat tgaggattaa ggaaggttcg caatgcccac cggtcgagtt
     1921 cgttggtttg acgcagccaa gggttatggc ttcatcacca gtgaggaagg caaggacgtg
     1981 ttcctgccgg ctcaggccct acccactggc gtcaccacgt tgcgcaaggg ggccaaggtg
     2041 gagtattccg tggtggacgg ccgtcgtggg ccgcaggcca tggatgttcg cctgatcgcc
     2101 tccgctccca gcttggtcaa ggccacccgc ccgaaggcgg atgacatggc cgccatctgc
     2161 gaggacctca tcaagatgct tgatgcggcc ggcaatacgt tgcgccgtca ccgttaccca
     2221 tctgctgccg acagcaaaaa gctggccacg ttgctgcgtg cggtggcgga tcaattcgat
     2281 gtacaggact gatctgatct atgactgata ccaccgaaac catcgtgaca ccggaccctc
     2341 acgccatcgc gcgcgccgtg ctgctcgaag tggccggcga gagcgatcag gtgggggatt
     2401 tcgttacctc atatgatctt gaggatcacg tcactgactt ccgcttcgcc gcgaatatcc
     2461 gtggctacga aggatggcaa tggtcggtga cgctgtatca cgacgaggag atcgactctt
     2521 ggaccgtcaa cgaatcctcc ttgatcccca ctgaggacgc gttgatgccg cccaagtgga
     2581 ttccttggaa ggatcgcctt gagcccacgg atctggctcc caccgattcg atcggtaccg
     2641 atccggatga cgagcgcatc gaggaaggcg aagtcgagga atcctcgctg caggatgtca
     2701 acgacgccgt cgagaccttc cgactgaccc gccgtcacgt gctgacctcg ctgggccgcg
     2761 cgcaggctgc caagcgctgg tatgaaggtc cgcgaggccc caaagcgttg agcaccaaaa
     2821 ccgccgaagg caacctgtgc tccacctgtg gtttcttcgt gccgctcgcg ggcgagctcg
     2881 accgtatgtt cggcgtgtgc gccaacaagt ggagtccgga tgacggccgc gtggtttcct
     2941 tggaccacgg ctgcggcgag cattccgaaa tcgaacctcc cgagccaagc cagctgtggg
     3001 tgcagtccaa gcctgctttc gacgatctgc atatcgacgt ggtggccaac cgtccgcgca
     3061 agcaggagcc ggctgcccag gacgaggccg aaggcgaaac cgacaagaca ggcgagcctg
     3121 ccggtgatga tatcgaagca cagaagacta ttgacgggaa cgccgtcgat actgagcccg
     3181 ctgaggaggc ggcaggcgac gggacctcgc aatcccagtc tgccggtgat gagtccgttg
     3241 cacagaacgt atctgattcc gttgtggatg ttgatgcagc tgattccagc gacgattcca
     3301 agtctgatgc tgacgacgag gccaccgaag aggatatcct caacaacacc gttgccgatg
     3361 atgatgagga tgatgagatg gacgacgaag aggataacgt tcgtccttca gacgatgtga
     3421 ctcctgaact cgaaaccgtc atcgatctca ttgagcagct gcgccagaac agggcggacg
     3481 aggaataaac tccgaattcc gcgaaaagta gatggtgatg gggttgcagg aataaagaat
     3541 tcctccctgc aagcccatca ccgaggcgtg cgttagcact ccatgccgat tttggtgaat
     3601 cagtgcacca cggtcaccgt gcattcggcg aagttcacga tctggcggga caccgatccc
     3661 aggaagtgcg cgtccagccc ggacaggcca cgcgagccga ccaccagatg gctggcgtac
     3721 cgcgaggccg cgatcagacc tttggatgcg ggaatatgga aggcgtttgt gctgaccttg
     3781 acgccatcgg gaatctcggc tttggccatc agctcggcga gaatctcctc ggcgcggcgc
     3841 tgaccgacct tcaccggtgc cacggcgttc tcatagcccg gaataacgcc caaatccttg
     3901 agctgccagc agaacatcac atnnnnnnnn nnacatcaca tgcagcggcg cgtcgtgcag
     3961 tcgtgcttct tccagtgcga aatcgaacgc acggcgggaa gtctcggaac catccacacc
     4021 aaccaccacc gggcggcgag cctcctcaac caccggctga ggctgcgtgt agtgaacggg
     4081 cttgtctccc ggagtcaggg cgttggcaat ggcctcggtc acggtctcat cctcgctgcc
     4141 ggcgatgcgc accacggtca ccggcacttc ggcggcctcg gccagcgaag cggacaggga
     4201 ccccatgaac cagcgggcca tacggctcag cgagcggcgg ccgaccacga tctgctgcga
     4261 atcatggccg atctgcagca gggccgaagt accggaggcc ttcaccgagg tgagcttcag
     4321 attcgccgga tcgaagtcga tgcctgcagc ggccttgtcg acccacacac gcagctcgtt
     4381 ggcaatatcg tggcggacct tggcccaggc ctcgtcgctg tccggttcgg accccatatc
     4441 ccaggaatgc gtccagccaa acacggcgtt gacgctctga ccggttaacg aggcctcctc
     4501 aagcgcccac ttcagcgcgg caaaggattc atcggaacca tccacaccga caacgatgtc
     4561 gggcttggtt gcagcttcac tcatttcgag cctccttgta atcgactacg ggtgaatcta
     4621 gaacc
//
LOCUS       DAWBMW010000122         4568 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_4021_length_4568_cov_3.250609, whole genome shotgun sequence.
ACCESSION   DAWBMW010000122 DAWBMW010000000
VERSION     DAWBMW010000122.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 4568)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4568)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4568
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4021_length_4568_cov_3.250609"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    910..919
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2147..2156
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          937 a         1443 c         1308 g          860 t
ORIGIN      
        1 ctctcccacc gtgaatgttg cacagcgcat gggcaaaacg cctaactatg ccgccaccta
       61 ccgcaaacgc ctccttgacg ccttcgttat ccgcgaaaca gaacgaggcg aagtcgattt
      121 tgccgtgccc ttcctgcgcg aatacttgcg tcggaatcag taatcggcac aatgcacagt
      181 atcgatatat tccgttccat ataatgactt ttcgcattca gatacacaat ggacggcctg
      241 ttgagtagca ctcagcaggc cgtccattct atttagctat tcagttatcg gagacacacc
      301 tcaggccttg accggagcgc cagcgacctg ctgggaggcc atcacgccga acaggtagcc
      361 gtcttcggag aagtattcga aggtacgacc ggcgaagtcg cggcattcga atctgatatc
      421 gttatcgatt cgatcagcgg ctcacgatac tgcccttgca tcgctgtgcc actgttgaat
      481 gtgcccggct ggctcacctt tgggtcaccg ccgacacatc cttgatgccg atggttatag
      541 agtcgcaaat tcacgactgg cgcacctgcc cacgcaccta tcctgtcatt tcgagcgcat
      601 aatttgctac cacatccggt cagggcaatc gaccttaatg gcgtaaacgg cacttctcaa
      661 tacgtaaacg gcatttattc ttggctcccc tctcagagag gggagccaag aaatatcaag
      721 ctcggcctcc caggagagag ccagaataat gcaccagcaa ggcattagga tggagggcat
      781 gagcgatttg aacgcgttga atcttgagtc cggcgaagtg gttggtgggt atacgctgat
      841 ctcccggctc ggctcaggag ctatggggtc ggtgtggcgg gtgcgcgacg acggcggcac
      901 cacctatgcn nnnnnnnnna tctttaccaa atttctgtcg ctgaacgccc caagcaccac
      961 cccaaagacg ccaccaagct aggatggagg gcatgagcga tttgaacgcg ttgaatcttg
     1021 aaccgggcga agtggttggc ggatacacac tggtctcccg actcggcgcg ggcgccatgg
     1081 gatcggtatg gcgtgtgcac gacgatggcg gcaccaccta tgccatgaag attctgcgcg
     1141 actccctgac cgacgagtca gtggcaggct ccggctctgc gtccacggcc ctgcacgacc
     1201 ccgacctcaa gcccgatgtc acgccgcgcg agcgcctgcg gcgcgaggcc atggccttga
     1261 agaaggtcaa tcatccgggt gtgtgcggca tcgtggatat ggaactcgac gatacgttgg
     1321 cgttcatcgt cactgaactc atcgagggca agaatcttaa ggatgatgtg gccgccaatg
     1381 gccggtatgt gggcgacgat ctggaacgct tggcccgcaa gctcatcgaa gccaccaaag
     1441 ccgtacatgc ggccggcatc gtccatcgcg acatcaaacc caccaatgtg atggtgtcgg
     1501 ccgccggacc ggtattggtc gatttcggta tcgcgatggg cgagggcgaa agtcacgtga
     1561 cccgcaccgg cctagtgatg ggtacacccg gattcatcgc acccgagatt attgacggtg
     1621 ccgaatccga cgacgccacc gattggtggt cggtggcgtc cgtgctcgct tttgccgcaa
     1681 ccggcgcgcc tgtgttcggc accaagccga tgatggcggt acttgagcgc gaggcctcgg
     1741 gcaacgcgaa tctttccgga ctaccggccg gcacattggc tgccttccgt tctgcactgg
     1801 acccggatcg cagggatcgt tgcacgccgg atcaattgct gaatgccatc gccttggatg
     1861 cgttgaatcc caacgcttgg cagggcgagc agacgggacc gttcgccgcg ggagccgtgg
     1921 ctggacaacc ggctgcgatc gttgccgaag ccgcggaggc gatgccccct tttggcgcgg
     1981 ccggcggcgt tgaccgcaat aatccccgaa ccatctggcg cgcggccgag gaagccgctg
     2041 aggctcccac catggccgtg ttctccggca ataagggcga taacggcgat ggcgaaaccg
     2101 tcgtattctc gccggctggc gccgctaccg ccacgctgcc cttagcnnnn nnnnnngccc
     2161 ttagccgata tgccgccacg tccgccggcc ggtgcggagc aggccacgca actgttgccc
     2221 gacgctgctg ccgatttcac cgacgacgcc ggcacacagg tactcaccgg cgcgaccaca
     2281 ccgatgccgg accaaaccac gccgctggcc gagcgcacca ccgtactccc cacgccgcag
     2341 ccgtcatcat tccccccgtc cggcaccgcc cagagcagct gggatcccgc aagcaaccag
     2401 cccacagcgg tggaccccgc cattcgcccg gcattgcagc gcatcatcga ccaaaccgcc
     2461 caagcggccc ccaccgaaac catggtccaa aatcctccca tgcccaatcc ggtggacgtg
     2521 aagcgagggg aatatctgca gcgcggcacc ctgccattga tcctgctggc ggcgccgctg
     2581 gcggcactgg cggcaaatgc tccgctcgtg gccatcatcg cggccactgc gttgttgtgg
     2641 ctactgctca ccctggccta taacaccgaa ccccagcttg agcgcgaggg caaacgcggc
     2701 ggcgagcgca aaaactccga ttcgtggatt cgcgtggcga cttttccctg gcatctggtc
     2761 aaagccctgc tgctggccat tccccgcata ctgctgctag tcatcatcta tatggcggga
     2821 accgccgtgg ccaccgcggc tctggcactg cccgttcaca ccatctcctg gtatttcacc
     2881 gcggattggg gtattccggt gccgttgctc aatgatgtgc cgttctccgc ttccgggctg
     2941 gcgctgggcg gtttcatggc catcggctgg ctggtcactg tgttcgcccc acagtcgatg
     3001 atgattcggc tggggggcgg cgtactgcgc gggctcaaac acagtcgccc ggcgggcgcg
     3061 ccatccaccg aagtgggtgg ggctccacct gctccagtgg cgccggcgcc caagggcggc
     3121 gggcgcggca ccgtgctgtt caccatatgg gccatcatca ccgtggcctt gtgcgcgatg
     3181 ccgattctgg gcatgccgat caactggatg ccgttcgcct agagcggggc ggaatctcac
     3241 acatcagcca tccgcacaca tggccgttgt gtatccgata cgttcgttcc gtcacacttt
     3301 tacagggaat tcacctcaga tcagctctgg gcgcagtcct cctcaggccc ggtcattatg
     3361 atggggccca aacattgaag cgattgcaag gggatagcta tgtcagatcc gaagcgtcag
     3421 gccaagcgtg ccacacgcgc tgagcgccga gcggccgagg agaccgcaca gcaggcacgg
     3481 gccgagcagg cagccaagga gcgtaagcag cagaccatca tcggctgcat tgtggtggcc
     3541 attattgtgg tgctggtggc catcgctgga ttcgccgtaa acaaggctat gcggccgtcc
     3601 aacaccagca gcagtagcca gcaaagcaac atgaccgtgg acgaggccta tagcaagctc
     3661 aagaaagtca gtacccagcc ggctaacgcc gacgacaagg ccggcttcgt aatctccaac
     3721 aaagggtatg gccagaaagc cgagggggct cccaccgtat ccatctacat ggaaccgctg
     3781 tgccccggct gcgcctcggt gaaccgccaa ctcgacccga cgctcgtcaa gctgatgaac
     3841 gcaggccagc tcaacatcga tttgcacttc ctcaatttcc agaacaacaa gagctccgac
     3901 aactactcca accgcgtgtt caacggtgcc atctacattg ccgagcatga tgacgatccc
     3961 gaccacctca tgagctacct ctccaacatc tacgctgagg atttccagcc gggcgaattg
     4021 tccaattacg agccggtaaa caacgccaag ctggagaagc aggccgtcaa ggccggcgtg
     4081 agcgaggatg tggccgccgc ggcgttcagc ggcaagaacg aatacctcga ctggctcacc
     4141 gcctccaaca actacacgat cctgcgcccc gaactgttca acagctccgg cgccttctcc
     4201 tcccccacac tcaccatcaa cggcgaatac tgggatctga agcagctcac gctggcggac
     4261 acgaatatgg tggacggctt cctcaagtcc attgggctgg atgctgacca ggtaggcgtg
     4321 gaaggcaaaa tgccatccat cggtgccagc ggaaagccga tttcggtcgc ctcctgatgc
     4381 tgcgtggccg aataagttga cgccgcgaca tatgcaatac cgcctgcggc tcggcttaaa
     4441 ttgaccactc gggaagtacc ggtatactag tcatttgttc gcaacgattt acgcgttaag
     4501 aacaatcgcc tctttagctc agatggccag agcggccgcc ttgtaagcgg caggtcgtcg
     4561 gttcgatc
//
LOCUS       DAWBMW010000123         4504 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_4086_length_4504_cov_3.330187, whole genome shotgun sequence.
ACCESSION   DAWBMW010000123 DAWBMW010000000
VERSION     DAWBMW010000123.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 4504)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4504)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4504
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4086_length_4504_cov_3.330187"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2630..2727
                     /estimated_length=98
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          936 a         1374 c         1168 g          928 t
ORIGIN      
        1 tagttcgtca gcagttcgcc agcggcgaat tacgctgcaa atcatagcgt ttacccgata
       61 aaacacgccg gccttccttt tccgcatcca gcaaactctc atataatctc ggtataagag
      121 acggcaaggg acgaaggggt cctcgtgcaa cttacgacga aggaggctgt catgttgaat
      181 ctcaagcgag gtatcggcac tcacgagccc agcgtgattt cactggggca agtctgggtg
      241 gacattatgc tcaatgtgga caaggtgccg gcacagggcg gattcgccgt ggccgatcat
      301 ccgaagccct ccattggcgg cagctatcgc gtgctgcagg cggccagccg catgggcgtg
      361 ccgaccgagc acgccggcat tctgggcaac ggcctgtggg cgcatttcat ccgccagtca
      421 ttccaagaca acggcattac gcatatcggc caggatcgtc ttgatgagga ttccggcttc
      481 cgcgtggtgc tgagctccgg cgcgccgaag aagacattca tcgcttccta tggcgccgag
      541 gctcatggcg actcggacac gttcgacacg ctggagccgc agcccaaaga cgtggtgcac
      601 atcagcggca acacactgat ggaccacacg gccaccggcg tggatggatt cctgctcaag
      661 gccggtaccg acccggctgc acgcgactac acgctggtga tcaaccccac caatacgttg
      721 cgtttggtca acgaccatat gcttgaggac ctggtgctcg cacgcccggt ctggtcctgc
      781 aaccgtcagg aggccatgac gctggccgag cgacttggtg cgccaatcga cgacagcaaa
      841 gtcaccattg gcggcggact tgacgacttc atgcaccagt tgtgcgacgg cttgggcgct
      901 acactgcgcg cgccgctggt ggtgcgtgtc ggcgcccgcg gcgcttggat tcgtgagcca
      961 ggtggcgaga cgattcatat cgagggatac ccgaccaagg ccgtacacac ccgttcggcg
     1021 ggcggttgcc acaccggcgt gatgtgcgcg atgctggcaa agggccatag tctggccgaa
     1081 tcggtaaaga tcgccaacgc ggcggcatcc atcgccatcc agcacagcct caacggcgtg
     1141 ccgcaatgcc ccgattacga cgaggccatc gcgctgatcg gcgaatagtc gactataggt
     1201 agctcccccc agtccgcttc gcggccagct cccctcagcg aggggagcta tttttgctct
     1261 ggctcccctt agaaagaggc ccggcattat tcatccgccg ttcaaccgga atggctcccc
     1321 taccctagtc cgcgcttcta cgatagattg agttgaatac tgtcgaggag tgtatatgac
     1381 tgatcccacg aatgtccacg aaccggtgcc gcaaagcgcc aaactgaccg cccgcgagaa
     1441 gaagtggatc atctacgacg tcggcaactc ggcattcgtg ctgctgtcca ccgccgtcat
     1501 ccccatttac gccaaatcgc tgatgccggc ggacggcaac atcgtgtccg cctggggcta
     1561 cgcgcaaacc attgcttcct tggtcatcgc gctgctcatg ccgttgctcg gctccatcgc
     1621 cgacgtgcaa ggcatgaaaa tcaagttctt ccttggcttc ttcggcactg gcgtggtcac
     1681 ctgctgtgca atggccctgc ccctgacctg gctaccgttc ctagtcgtct acattctggc
     1741 gaccatcggc ctcaacggct cgctaacgtt ctacgattcg atgctcatcg acgccacctc
     1801 gaacgagcgc atggacaagg tctcctcgca tggctacggc tggggctaca tcggctccac
     1861 cgtgccgttc atcttctgca tcgcgctgat cttcggcggc ccgtcgctct tcgactggtc
     1921 cacggtggct tgcactcgcg cctcattcat catcaccgcc atctggtggg tggcattcac
     1981 catcccgctg cttaccagct accgccaagt gcactatcgc gccacccgtg accagctggg
     2041 ctcggccatg cgcggcacat tcagcgaact ggcgggcacg ttcggcaaga tcgtgaagaa
     2101 caagccgttg tggatgttca tgatcgcgtt cttcttctac atcgatgccg tgaacacggt
     2161 gatttcgatg agtacctcct acggtgccga gcttggcatc gactccaccc agctggtggt
     2221 ggcactgctc gtcacgcagt tcgtggcctt cccctgcgcg attctctacg gccgtctggc
     2281 cggccggttc ggctgcaaag tgatgatcac ggcggcgctc gtggcataca tgtgcatcgt
     2341 gctcttcgcc gcgttcttca tgaaatcggc cgtggagttc tggattctgg cgattctggt
     2401 cggcatgttc caaggcggca tccaggcgct ctcccgctcg tactacggca agatcatccc
     2461 caaggaccac gccaacgagt actacggctt ctacgatatc ttcggcaaga ccgcctcgat
     2521 catcggcacc ttcctggtgg ccaccaccac gtccctgacc ggcaacgcct cgttcggcgt
     2581 gctgtcaatc gcaatcctgc tgatcgtcgc gctcatcttc ctgctcctgn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnacg caagcgtaat atcatccatc aatcagcaac
     2761 aggaacgccg aagcagcgat aaaacggcag attctaccgc ccgagccgcg acctgaaaca
     2821 atttagccct gcacaacgca catattttga cagggcggta gttgtcgcaa attctgcgac
     2881 aactaccgtt ggtaaatcga acgctataat cagaaacttt cagatgctga gttccatgca
     2941 gtaccccctg tgaccgcaat gcgggcaggt aagcttgcgt gtcttgggcg tgtgtgcggc
     3001 gaatacgaac tctcgcatac ccggctggaa ggtctcatgg caagccggac ataggtactg
     3061 cacgcgggag cgatagtact tcatcgccgc aacagtgagc gcaatcgcca gcagaacggc
     3121 gaccggcagc ggccaccata cgtctgtcat cacaccgatg acgaggcatg caatttgcaa
     3181 caaagtcgcg ggcaatgcga ttgccagcat gaccaaatat gtggtgcgca gggaatgagt
     3241 ggttgtggtg ttcataacgg ttgccatgtc ggttaatgtt gacgatacga agtcagacgc
     3301 cgagaccgtc agtgctttgc gcagctgcaa gcagtcatca agacgttgct tctgctcggc
     3361 gatacccgac tgcaaggatg ccgtctgctg atccagcaaa tcaaccagca cccgttccgg
     3421 ccgctcgtcc gacagaatcg agcgaatctg ctccagcttg aagccgagtc ctttgagaaa
     3481 aaggatgacc cgcatccgct cgacctctgc agccccatac agccttcggc cgccgtcgct
     3541 gatctcattg ggaatcaaca gacctttggc gtcgtagtac tgcacggttc gcacactcac
     3601 accagtgagt ttggcgatgt cccctgtggt gtatcggctg tccgtttcat cgtccatcga
     3661 catggcctaa cctcctttca ccaagcaata tgccgcatta cgcaacgtca caagcaacat
     3721 atgacgcaac gtcgaccaca aaaccttcac atataagcgg actgtagaaa gacatccgac
     3781 catcgcaatt gcgcactggt tcccagtgag taccccgaac cagttagacg gcagaacaga
     3841 attacagcca cacctgttct tcaggcttta cctcgccgac cgcattggcc acacgtatgg
     3901 catcattccg actcaatcta ccggtgaatc cctgtagatc cgaccggagg aaccgcaccc
     3961 gcatgcgata cacgtcggag acatgcaatt gctccggaac cggcatcaat tccggaaatc
     4021 atgattattc gccatcgcgc cacgccttgg gcagtacggc aacaccatcg gcgccccggc
     4081 ttggattcaa caccatggcg ttcggcactt cgaccaccgg atatccctgt tcgtcgaccc
     4141 cacggtacgt aactccattg gcggcaacat gtcccgcgcg aggcagcacg ggttcaggcg
     4201 ccttcacctc gaacgggata cgacgctgcg acaccaactg cacgctggcc atattcacga
     4261 acgtattgaa attcaatccc atggaatcaa gaatggcgtt cacttcagct ttgagatcat
     4321 catccatgcg aacagtggta gtgctcataa tgaatccttt ccaaagccaa ttgtattact
     4381 aatgacttct tttctcaata taatccgtag gatgcttaac gcttttgcat catgaccttg
     4441 acatcgggac aatcccgcgt gaaaccaatc tctgcctttg cgctcatcag ctggggagct
     4501 cacg
//
LOCUS       DAWBMW010000124         4482 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_4111_length_4482_cov_3.433025, whole genome shotgun sequence.
ACCESSION   DAWBMW010000124 DAWBMW010000000
VERSION     DAWBMW010000124.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 4482)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4482)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4482
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4111_length_4482_cov_3.433025"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    218..227
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          838 a         1295 c         1446 g          893 t
ORIGIN      
        1 atggtgggga tgttgaagtg ttcggcgagc agagcagcct gggtgccctt gcccacgccc
       61 tgggggccca tgatgagcag acgcatggtg gtctcctttt atgtcggtta ttaatacggt
      121 tttggctccc ttggttaagg gagccattga gttatatgcg gaagaaagat tgctccgtcg
      181 tggaacagtc cactggactg ttccactcct cgcgcggnnn nnnnnnnccg cctgcccatt
      241 gcgggcgacc gctgccaagc gggcgtcagc cctctttgtg gtccacgttt tcgaacagga
      301 agccggtgta ctggaactgt tcggtctggg ccttggcctg gcgcagggtg tcgaggccca
      361 cgcccgcgat aatcaggatc gtggtgccac cgaacggcag cttggcgttg aggccgaggg
      421 ccatgatcag cacggtcggg atcagggcca cgaacagcag gtagacggcg ccgacggtgt
      481 tgagtcggtt catcacgtag gtcaggtagc ggctggtggc gttgccggcg cggatgccgg
      541 gggtgaagcc gccgtactgc ttcatgttgt ccgcggtctc gtccgggttg aacgtgatcg
      601 aggtgtagaa gaagcagaag aacacgatca tcagcgcgta cagggcgatg taccacacgg
      661 atgtggtgtt cgccaggttg gcgttgatcc acttgaccca cgactggtcg gacttgccga
      721 actgggcgat cagggtcggg atggcgagga tcgaggaggc gaagatcggc gggatgacgc
      781 cggacatgtt gatcttcagc ggcaagtagg tggaggagcc gccgtacatc ttacggccga
      841 tcatgcggcg cgtgtactgg accgggacgc ggcgctggca cagttcgacg aagtcgacga
      901 agatgaggat gaccacgagc acgcccacga cgatgccgaa cttgagccag tcgccgttct
      961 tgccgttggt gccgtagccg atctcccaca attggggcag gaagccggag cagatggaca
     1021 tgaagatcag gatggacatg ccctggccga tgcccttgtc ggtcacgagc tcggccatcc
     1081 acatgatcag gccggtgccg ccggtcatga tgaggaccat gaccacgagg ttgaacacgg
     1141 aaccgtccgg gatgacctgg tcgcactggt aattgaacag ggcgccggag cgggcggtga
     1201 ccaggatggt ggtggactgg agcacggcca ggccgatggt caggtaacgg gtgtactggg
     1261 tgagcttggc ctcgccggac tggccctcct tgtgcagggc ctcgaagcgg gggatgacca
     1321 cgcgcagcag ctgcaccacg atggacgcgg tgatgtacgg catgacgccc agcgcgaaga
     1381 tagacagctg gagcatggcg ccacccgaga agaggttcac cagtccgatg aagttctcct
     1441 gggaggcgct gccgatggtg gccatgcact tgttcaccac gttgtagtcc acgccagggg
     1501 tcggaatgaa cgaaccgatg tggtagacga tgatgatgaa caggacgaag agaatcttct
     1561 tcctcaactc tttggttttc agggcctgga ttagtgtcct cacctagatt tcctcccaat
     1621 agcgcgccaa tcgcgcgaaa tacacaaact aacaaacgag catagtctaa agtgcctact
     1681 tcggccactt catgtctcgc gaatgttacc tgaattatgc tccgtcgtac tgaacggggg
     1741 gaagcatact caaaacgatc attgtgttga gactgcttct ctgcactcag aacaacaatt
     1801 ttttagaaaa gggaaccccc gtccacctat tggcgggcgg gggttgtaca gcctattgcg
     1861 ctccccgtag gactaatcag tcctcggaaa cggaaccgcc ggcggcctcg atcttggact
     1921 tggcggaagc cgaagcctta acacccttga gggtgaaagc cacggtggtc tcgccgtcgc
     1981 ccagaacctt caccggagcg ttgtcacgaa cagcgccctt ggcgatcagg tcggccacag
     2041 caacctcgcc gccctcgggg aagagctcca cgagacgagc gatgttgatg acctggaact
     2101 cgaccttgaa cgggttcttg aagccacgga gcttcggcag gcgcatgtac agcggcagct
     2161 ggccgccttc aaagcccgga cgcacgtggt tgcgcttcgt ctggcccttg gcgccacgac
     2221 cggaggtctt acccttggag ccttcaccgc ggcccacacg ggtgcggtcc ttcttggcac
     2281 ccggggcggg cttcaggtcg tgcatctgca ggatttcgtt atctgccata atcagtcaac
     2341 ctcctcaacg gtgaccaggt gacgcacggc gttcaccaga ccacggttgg ccggggtgtc
     2401 ctcgcggaca acggtcttac cgatcttgtt gaggccgagg gtcttgacgg tggcgcgctg
     2461 agccggggta cggttcacga tgccgtggtg cagggtaatc ttcaggttct tagccatgag
     2521 tatcactcac catccttctc ttcggcggcc ttagcggcag cagcctcttc gcgagccttg
     2581 cgagcctcgg caatgccggc ggcgcgggcg cgcagcagag catcgggagc gacctcgtcg
     2641 agcgccaggc cacgacgggc ggcgatctcc tcgggctctt cgagctgctt gagggcgtcg
     2701 acggtagcgc gcaccacgtt aacggcggtg gcggagccca tcgacttggt caggacgtcg
     2761 gtgatgccag cgcattccat gacggcgcgc acggagccac cggcgattac accggtaccc
     2821 ggggcggccg gacgcagcag gacggtgcca gcagcgtcgt gaccctgaac cgggtgggtg
     2881 atggtgccgc ggacgcgagg cacagagaac atgtgcttct tggcgtccag ctggcccttg
     2941 gcgatggcgg ccgggacttc acgggacttg ccgtagccga cacccacggt gccgttgccg
     3001 tcgccgacca caaccagtgc ggcgaagctg aaggtacggc cacccttgtg ggtcttggac
     3061 acacggttga tggtcaccac gcgatcgagc agctcgtcgc cgcggttttc ttcacgacgg
     3121 ttacgacgct cgccacgacg gccttcaccg cgctggccac gacgaccctt gcggtcctcg
     3181 ttgttggatt cggtcgccac agtgttctga gtttcttcag ccacttgggt ttccttcgtt
     3241 tcgttgtcgc tcacagtgca agacctccct cgcgggcgcc atcagcaacg gctgcgacgc
     3301 gaccggtgta cttgttgcca ccgcggtcga acacgactgc ggtgatgccg gcagccttgg
     3361 ccttctcggc gatcagctcg ccgaccttct tggcggcctc agtcttggtg ccttcgaagc
     3421 cggcgaagtc ggcctgaaga gtggaggcgg acaccagggt aatgcccttg gtatcgtcga
     3481 ccacctgagc aaccatgtga cggttggagc gggtgaccac caggcgcgga cgctcggggg
     3541 taccggagat gcgcttgcgg atacgcgcgt gacggcgctt gagagctacc ttcttgcctt
     3601 ttccgagaat agcaacgctc atatcactta ccagcctttc cagccttgcg caggatgcgt
     3661 tcatcggagt acttgatgcc cttgcccttg tagggttccg gagcgcgcag cttgcggatg
     3721 ttggcggcaa cctggcccac gacctgcttg tcggtgcctt cgacaaccac gtggttggcg
     3781 tcggtcacct tcagggtgat gccttcgggc gggttgacgg tgatggtgtg ggagtagccg
     3841 aggaagaact cgatgccctg gcccttggcg acggcgcggt aaccggtgcc gacgatctca
     3901 agagtcttgg agtagccatc gtgaacaccc ttgaccatgc cggccatgat ggaacgagcc
     3961 aaaccgtgct tggcacgggt cggacgcagg tcatcagcgg cggtcaggac aatctcgttg
     4021 ccttcaacgg cagcggtgat gccttccgga accacgtagg agtcagaacc cttagcgccc
     4081 ttggcggaga agttctggcc ttcaatcttg acttcaacgc cagcagggat ggcgatgggg
     4141 agcttaccaa tatgcgatgc catgtttcag ctctcctttc tcaccacacg aaggcgacaa
     4201 tttcgccgcc aatgcctcgg tcgaggcatt ccttctgagt cagcagtccc gaagaagtcg
     4261 agatgatggc gatacccagg ccaccgagcg gcatcggcag ggagtcggac ttcgcgtaac
     4321 gacgaaggcc cggcttggag atgcgcttga taccctgaat ggaacgctca ccgttcggac
     4381 cgtacttgag ggtgacctca agagtctggc cgaccttggc ttccttagcg gtgaagtcct
     4441 tgatgtagcc ttcacgcttc aggatctcgg cgatgttcgc ct
//
LOCUS       DAWBMW010000125         4463 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_4139_length_4463_cov_2.009982, whole genome shotgun sequence.
ACCESSION   DAWBMW010000125 DAWBMW010000000
VERSION     DAWBMW010000125.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 4463)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4463)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4463
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4139_length_4463_cov_2.009982"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    385..394
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3927..3936
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          796 a         1243 c         1476 g          928 t
ORIGIN      
        1 cgatgttctt ggtggccagg tcggccaccg ggccgccgtc ctgccagatg tcctcgcaga
       61 tggccacgcc aatggtggcg ccgtccacgt cgagggtcac ggagcggtcg ccggcggaga
      121 agatgcggaa ttcgtcgaac acgccgtagt tgggcaggaa gtgcttgtcg tagccagccc
      181 agaccacgcc ttggtgcaag accaccagtc ggttgcgcgg cttggagttc ttgcggtcgg
      241 tgcccacggt gccgaccacc acgaacagat cgccgaggcc gtcgtcgttg agctgggtgg
      301 cgagttcgtt ggccttgtcc catgcggctt ggcggaaggt gcggcgcagg gccagatcct
      361 cgatggggta tccggtgagg gtcannnnnn nnnncaacca cttgggcgtt tcctgcggcg
      421 gctttacggg agtagtcgag caccttggcg gcgttggcgt caagcgcgcc gacgcaggtg
      481 tcgatctggg cgagtgcgaa gcgaagctgt gtcatagatt cttagtgtag ccgcgtatga
      541 cgtcggattg cctgttcttc cggcttggcg tcattcatta ggtcgttttt gcgttcatta
      601 ggtcactgca atgcgatatc agtcgttgca atggcgcgat tccaacaacc agtatcgccg
      661 ttcaccgacc taatggacga tgaatcgacc taatgaacga atgccactcc cctcagtcag
      721 cttcgctgat agctcgtcct gcacttatgg acgggcgagg cccgcgcata tctggttcgg
      781 ctgtcgcctc accttcggct acagacagcc cactgggctg tctgcctaac gcctcagcct
      841 ccggaggcga gccagaaaag gatttacttc agctcgttga gcacgcgcag agcggtgttg
      901 acgtagaagt tgacgaaggt gggaatggat tcgtcgagac ccacgaaatg cgggctatgc
      961 cagtcggcgc aaccttcctg gccgtttgac ccgacgaacg cgaacaccgg caacgtcacc
     1021 ttggcgaatt cgcagaagtc ctcccccgcc atcgactgac gtatcggttc gagctgcgcg
     1081 tagtcgttga catccgccgc cacggccttg gcgagacctg gatcggagac cagcgggtct
     1141 tgaaagtcat cccagaccac gtcggcgctg atgccgtatg cctcggcggt gtgttccacc
     1201 acttccttga atcggcggcc gaccagattg ccatccgact tgtggaaata gcgcacagtc
     1261 ccttggaatc cggcttcggc gggcaccacg ttccacacat ggccgccgtg cacttcggta
     1321 acggacagca ccagcgggtg gaagggcgtg acgttgcggc tcacgatggt ctgcaggctc
     1381 agaataatac tggcgagcgc ttcgatgggc ccggtgccct tgtacgggta accggcgtgg
     1441 gtgccttggg cgtggagggt cacgcggaat ttcacgcagc cggccatcat cggctcaacg
     1501 ccgatggcca gctggccggg cgcatagttg gggttgttgt gcgtgccgat aatggcttcg
     1561 acgtcatcca cgagtccggc gtcgatcatg gcgcgagcgc cttggccggt ctcctcactc
     1621 ggctggaaca gaatcttgat cgagccggcg aacttgtcac ggtgctcggc gagccagaag
     1681 gcggcaccga gcagaccggt catgtgcagg tcgtggccgc agccgtgcat cacgccttca
     1741 ttgacggagc tgaactccag tccggagtct tccacgatgg gcaggccgtc gatgtcagcg
     1801 cgcagcccga tacgcggccc gggaccggcc tcgccctgaa tgagccccac aacaccagtc
     1861 tccagcgggt tgtgcaggat ctcgatgccg tgcgccttga gctggtcggt cagatagatg
     1921 ctggtgccga attcacggaa actacgctcc ggatgggcgt gcaggtagtg gcggatagtg
     1981 atgagttcgt ggtcgatgtc gatatgttca ctcatggttc ccgattctag tagcgcgaag
     2041 aatatattgc ggccctcgct ccatattggc gagggccgcg atctatgggg tcagtatgcc
     2101 ttgttattgc tggcgtttga gcagtcgttt ggcgatggcg ttggcgatgc cctgcaccac
     2161 ctggaccagt acgatcatga tgatcacggc cgtccaggtg acggtctggt cgaacttctg
     2221 gtagccgtag gcgatggcga agttaccgag accgccaccg ccgatgtagc cggccatggc
     2281 agacatgtcg agcacgccaa tgaacaggaa ggcgtaggcc aggatcagcg gtgccagggc
     2341 ttcggggatg agcacggtgc ggatgatggt gagcttgccg gcacccatcg aacgcgcggc
     2401 ctcgattact cccgggtcga ccggcacgag attctgttcg accagacgcg aggtggcgaa
     2461 ggtgcacatc accaccatcg ggaagatggc ggccacggtg ccgatggacg tgccgaccac
     2521 gacgatggtg agcggctgca tggctgccag gaagatgatg aacggaatcg ggcgcacgat
     2581 gttgacgatg atgtcaagga tgcggtagac gacctcgttc tcgaacaggt tgccggggcg
     2641 ggtgccatag agcaccacgc cgaggatcag gccgaggaat ccgccgatga cgagggtgac
     2701 gagcaccatg gtcagggtct ggccaatgga ttcgaacagt agtggacgca gcacgttcca
     2761 gtcgctacgc gggttggcga ggatgatcat gcgttggctc cttcatgtgc ggcggcggac
     2821 gcggcgggcg cggtggcagt atcggccgcc agtgggtcat gggcgcggtc ttcggcgtag
     2881 gaggcatgtc cggcgactgc gtcggcgtag gccacaggct ttgcggcggt gccgaagtcg
     2941 atgacgtcgc tattggcggc gagctcgcgc aggaattcgt ccacatgcca gccgggcccg
     3001 ttgaattcgt aggtgatggc accgatggcg gtccccttga ccgtgtcgat gccgccgtag
     3061 agcaggctgg attcgacgcc gtatttggcg atgagttcgg agatattctg tccggatgcc
     3121 ttgagttcat ggccttgggt gccggaaacg tccttttggc ggataagcac ggtgacaatg
     3181 cggcccgacc attcgtgatg caatcgctcg acccggtctt cctcgggcag gccagacaat
     3241 gcggtggcaa tgaatcgctt ggtcaccggc tgctggggtg cagcgaacac atcgtatacg
     3301 tcgccggatt cgcccacgcg tccggcgctc attacggcca cgcggccgtc aatctgctgg
     3361 accacgttca tctgatgggt gataagcaca atggtgatgc cgaattcctc gttcacacgc
     3421 ttgagcaagg ccagcacttc ggtggtggtt tccgggtcga gcgcgctggt ggcttcgtcg
     3481 gcgagcagaa tctccggatt agtggccagt gcgcgggcga tgccgacgcg ctgcttctgg
     3541 ccgccggaca gctgcgaagg gtatttgttt gcgtgctcac tgaggcccac gaattcaagg
     3601 agttcgttga cacgacgatc ctgatagtcc ttgcgccagt gatcgagttg cagtgggtag
     3661 gcgatgttct gggccacggt tttggtggag aacaggttga actgctggaa aatcatgccg
     3721 attttctggc ggatgggccg cagcttggtc tcactcagag aggtgatatc agtaccaagc
     3781 acggtcacag tgccggaggt gggtcgttcg agcgcgttga taagccggac cagtgtggat
     3841 ttgcctgcac cggaatagcc gatgatgccg aagatgtcac cttcgttgat ggtgaggctc
     3901 acatcgtcaa cggcgcgagc aacggtnnnn nnnnnnaccg cgatcgatgg tcaggctgac
     3961 gtcatccacg gctcgtgcga tgttcgcgcc gcgtccgcgt gttttgaatt ccttcacgac
     4021 atggtcgaag gtgatgatgg ggtcgctcat agtgctccct gatgctggtt gttggtgtcc
     4081 ggtcacggaa gcggagcgaa tcccgattgt tggctcgggt actcgctccg cttggtggta
     4141 atggctatgg acaggctagt cgtgcgctcg tatcagccgc ggctcactgc tgggccttgg
     4201 catccttctc aatgtcggac aggaagcctt gcagctcgct ggcgctgaac ttgtcggcga
     4261 acaccgcggt accgccggag ttctcctgca gggcgtcgag cacatccttc tgctggtaga
     4321 tggaaacgag cttcttgtag gtctcgttgt tcacgtccgc cttgcgggcc acccacacgt
     4381 tgatgtaggg gcgagcctct tcggattcgg cgtcatcctg gtagatggcg tccttcgggt
     4441 cgagaccggc atcggccacg tag
//
LOCUS       DAWBMW010000126         4455 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_4150_length_4455_cov_2.735682, whole genome shotgun sequence.
ACCESSION   DAWBMW010000126 DAWBMW010000000
VERSION     DAWBMW010000126.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 4455)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4455)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4455
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4150_length_4455_cov_2.735682"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3574..3583
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          958 a         1285 c         1352 g          850 t
ORIGIN      
        1 gctcagctga gtgccaaggg cctcgatctt cttgcgaacg gaatcgaaac tcacggaaca
       61 ggaaccgttt cgacgatctt gtcgacgatc tgatcgctga ccacgccatc ggccagtgcc
      121 tcgaacgtca gcataatacg gtggcgcagc acctcatgag cgaacgcctt gaggtcctcg
      181 ggtaccacat agtcgcgccc ggacagcaac gccttcgcct ggccgatacg ggtcaaggcg
      241 atggtggcgc gcggcgaggc acccaaacgc accaaggatg acagtccctt gatggggcgg
      301 gagccggcac ctcgtgacgt ggcggtgata tcgaccgcat aacgcatgat cgcgtcggaa
      361 acatgcacgc ggcgtgccgc cttgcgcaaa aaatcaacgt cggacacgga aatgcgttcg
      421 ccggtgatgg tgtcgggacc gatcatgtcg gagccgcggc gggtgagcat ggccagcatc
      481 cgagcctctt cgtcgggcga aggataggtc atgacggcct tcatcatgaa acgatccatc
      541 tgggcttcgg gcaggttgaa cgtgccctcc tcctcgatcg gattctcggt ggcgatcacc
      601 atgaacggct tgggcaaggc gatacgctgc ccgccgatgg tcgtggcacc ctcggccatg
      661 gcctcaagca tggcggactg ggttttggca ttcgaacggt tgatctcgtc cagcagtacg
      721 aaattggcgt gaatcggacc gatctgcgtg gtgaacttct gcgacgcaaa atcgaagacc
      781 tgcgtgccca ccagatcaga tggcatgaga tcaggagtgc actgcacgcg cttgaacgag
      841 ccggatacgc aggtggcgag cgtctgggcg gccgtggtct tggccaagcc cggcacggat
      901 tcgataagaa tatgcccgcc ggccaccaag gtcacgataa gcgattcgcg tagattatcc
      961 tgacccacca gtgtctgggc gaacctcgcc cgaatggcgt cggccaggat acgtgcgcgt
     1021 ttggcgtcat cggcacccag cggagccgac acgccgggcg cgggaacggt gaccggtcgt
     1081 gcggcggtgg tcggcgaagg tatgttcggt tgcgtgggga acaaagtcat gccccccact
     1141 gtaactactc acaatgactg ttggcctgcc taaagagtcc aatccaccgg gtcggcaccc
     1201 atggtgacta gggcttgatt ggctctggag aatggtttgg agccgaagaa tccgcgggaa
     1261 gcggacagcg gactcggatg aggcgatttg ataatgaagg cattggtcag caatggctcg
     1321 aggctttgcg cattacgccc ccagagaatc gccaccaacg gcttgggctt gccgtcggcg
     1381 tccgtgcgcg cgttcaacgc acgaatcgcg gcctcggtga cttcctccca gcctttgccc
     1441 tgatgtgaat ttggccgtcc gacgccgaca gtcagacatc tgttgagcaa catcacccca
     1501 tgttccgtcc acggtgtcag atcgccgttg gtgggcatgg gcaccccaag gtcgtcaacc
     1561 aattccttat aaatattgat caggcttttc ggcagcgggt gaacatttgg cgccacgcag
     1621 aagctcaaac ccaccgggtg acccggggtc gggtaggggt cctggccgac aatcagcacc
     1681 ttgatggaat cgaacgggat ggtgaacgcg cgcagaatat tgtggcttgc cggcagccac
     1741 gggcgcccgg cggcgttttc cgcacgaaga aaatcgccca tatggtggat ggtgggctcc
     1801 acgtcggcca aggcatgggc ccaccctggc tctaccagtt cgctcaatgg tttgatcatg
     1861 actcaccact gtagtcaaac gtgcatatcc ggtgcatggg gcgtggtgtg gtggactgat
     1921 gagttgtttg ccgattacac tcatgggtag ttttgatagg aagaaagggc gattatggca
     1981 cgcgacacga tgacctacga atcctatgaa ccggattcct tcgacaatcc tccgaaggga
     2041 ccggtgggag ttcaccgtgg cgctcgttcg gtaatggccc gcctctgccc gttcatcgtc
     2101 gttattctgg tggccgcgct gtgcggtgtc ggggcctggg catggatttc cggagaatac
     2161 aagaatgtag tgggaggatc gtcccagacg gcgaccacgt ccaagtccga ttcgtccagc
     2221 aaggctgaga ccaagaacag caccaaggat gattcctcgt ccacttcgac cgattcgtcc
     2281 aaggactcga cctcggattc ggcaaagtcc gataccgaca gtgacacgac atcctcgaat
     2341 gagaatcaaa actccgatca gcagtctcag cagtccgaag ccaccgcgac tgtgaataag
     2401 gccacgcagg tgcgtgtggt caacgccaca ggaatccaag gatatgcggg tcagcaggcc
     2461 gatgtgctgc agaccgccgg ttacaccagt gtcgaggcga ccaatccgag cggcacattg
     2521 ccggcttctt ccgtggtgtg gtatcagaat gagaccgata aggccaccgc cgaggatgtg
     2581 gcgaccacgc ttggcatttc ggctgtggag caggtgcaag gactcgccgt tcccattacg
     2641 gtggtgttgc tcaactagcg ttgttgatgc gcccctgata tcggcgagaa atacatcgaa
     2701 aactgtgagc gttctcacgc ctatggaata attcgtaccg aaaatgtgcc actgcggttg
     2761 agattgttcc ctcgtcactg tacaatcgtc atctagtcgc gaggtagggg gagcgcgatg
     2821 cggttctttc tacgttccct ataggttttg tgtcgcgacg cccaagggaa caaaggaaag
     2881 accagatatg gcacaaggta ccgtgaagtt cttccttgcc aagaaggggt tcgggtttat
     2941 ccagccggac gacggcggcg aggacgtttt cgtgcactac gcggaaatca aggacgacgg
     3001 cagcaccaat aagttcaaaa tgctgtacga aggcgatcgc gtggagtata cgcccgcttc
     3061 gtccggcaaa ggcacgcagg caaaggatgt tgtcaagctg tcttcgggtg aagcccgctc
     3121 cgagtgagca acacgcacgc aaggatgtgc attgaaggcc ctgaccgttt cgacggttgg
     3181 ggctttttca tgcccaaagc gcaatcgccg ccgatggtgt tggcactcgg cgggcgcgag
     3241 tgctaatgtc tgcgttagca ctcggaggtc gagagtgata atcggcagct gacggctggt
     3301 cgtcacgact cgacgcttgc gagtgataac cggtatacct agccatttgg aggaatacgc
     3361 aaccatggca aagatcatct cttatgatga ggaagcccgt cagggcatgc tggagggcct
     3421 tgacaagctc gccaacaccg tcaaggtcac tctgggcccg aagggccgca acgtcgtgct
     3481 cgacaagacc tacggcgctc cgaccatcac caacgatggc gtgtccatcg ccaaggaaat
     3541 cgaccttgaa gacccgtacg agcgcatcgg cgcnnnnnnn nnnctggtca aggaagtcgc
     3601 caagaagacc gacgacgtcg ccggtgacgg caccaccact gcaaccgtgc ttgctcagtc
     3661 cctggttcac gagggtctga agaacgtggt cgccggctcc aacccgatcg ccctgcgtcg
     3721 cggcatcgag aaggccaccg aggtcatcgt caaggaactc gtcgctgccg ccaaggacgt
     3781 cgagaccaag gaccagatcg ctgctaccgc caccatctcc gccgctgacc ctgaggtcgg
     3841 cgagaagatc gctgaggctc tggacaaggt cggccaggac ggcgttgtga ccgttgaaga
     3901 caacaaccgc ttcggcctgg acctcgactt caccgagggc atgcgttttg acaagggcta
     3961 catcgccccg tacttcgtca ccaacgctga cgaccagacc gcagtgctcg aagatccgta
     4021 cattctgctg acctccggca aggtctcatc ccagcaggac atcgtgcacg tggccgagct
     4081 ggtcatgaag accggcaagc cgctgctgat catcgctgag gacgtcgacg gcgaagcact
     4141 gccgaccttg atcctgaaca acatccgtgg caccttcaag tcctgcgccg tcaaggcccc
     4201 gggctttggc gaccgtcgca aggccatgct gcaggatatg gccatcctga ccggtgctca
     4261 ggttgtctcc gacgaactgg gcctgaagct cgagtccgtc gacacctccg tgctcggcca
     4321 cgccaagaag gtcatcgtct ccaaggacga gaccaccatc gttcagggcg ctggctccaa
     4381 ggaagacatc gacgctcgcg tggcccagat ccgcgctgag atcgagaaca ccgactccga
     4441 ttacgaccgt gagaa
//
LOCUS       DAWBMW010000127         4426 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_4176_length_4426_cov_2.902997, whole genome shotgun sequence.
ACCESSION   DAWBMW010000127 DAWBMW010000000
VERSION     DAWBMW010000127.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 4426)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4426)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4426
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4176_length_4426_cov_2.902997"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2076..2085
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2408..2417
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          915 a         1328 c         1228 g          935 t
ORIGIN      
        1 actcaagact gaccgccgtc tgtggcacaa tgacccgcag accaaacgaa cacaaaggat
       61 caatcaagtg gcagattatt acgagacgct gggtgtggaa cgcggcgcca gtgacgacga
      121 gatcaagaag gcataccgca agctcagccg taagtaccac cccgacatcg cgggccctga
      181 gttcgaggac aagttcaagg aagtgaacaa cgcctacgat gtgctgtcca atccggacaa
      241 acgccgtatg tatgattcgg gagtcgaccc gaacaacccg aacgccggtg ccggcggctt
      301 ctccggggcg ggcttcggcg atatgagcga cgtgttctcc acgttcttcg gttccgcgtt
      361 cggcggtggc tcacagggcc cggtgccgcg tacccagccc ggccgcgatg ccttggcctc
      421 ggcgtccatc gatttgaaga cggcggtgtt cggcggcacc gcgcatgtca agatcaatac
      481 gttctccctg tgccaggagt gcggtggctc cggtgcccag ggcggcgcac agccggtcac
      541 ctgccccgac tgccacggac agggattcat gcagaaggtc gtgcgcacca tgctcggcca
      601 gatgatgacc tccgccccct gtgaacgttg cgagggccac ggtaccatca tccagaaccc
      661 gtgccccagc tgcatgggcc acggccgtgt gcgcaccacg cgtaccgtgg gcgttaccgt
      721 gcccgccggc atcaacgaca acgcgcgtct gcggttggcc aaccagggcg aagtgggtga
      781 gggcggcggc gctgccggcg acctgtacat cgacatccgc atcaaggcgg ataagcagtt
      841 cacccgcgat ggcgacgacc tgcactgctg gattcaggtg ccgatgagct gggccgtgct
      901 tggccacgat ctgtccattg atacgttcga tggtgagaag accgtgtcca tcccggccgg
      961 ctgccagact gaggacacgg tgacgcttaa gggactgggc gtcacgaaca tccgcaacaa
     1021 ggacgagcgc ggcaacctca tcgcccacgt caatgtgctt atccccacca agctcaacga
     1081 aaccgagcgt ggcctcatcg aacagttcgc cgcctcgcac gattcaggcg ccacgcacgt
     1141 ctcccaggca tcccgtccac aggccggtca aaagaaaggt ttcttcagca agttgaagga
     1201 cgcactgagc tgagagcgga tgcgttcttg gctccccctc tgaggggagc caagtctgtt
     1261 acttcagcag ggagcggcac atggagcggt attcgttgac gtagccgccg ccgaagaaga
     1321 cgcagtggcc ggcgatgggg tagagctgcc agagcgtgat gcgctcctgc catccggcct
     1381 tcaatggatg tacggactgg tagccctcgg tgatttcgct cagatagctc ataccgaaca
     1441 ggtgcagcat agccagatcc tcctcgcgat gaccgccatg tgccgccggg tcgatgagca
     1501 cggcctcgga ctgcccggaa tcggcggtcc acatcacatt gccgctccac agatcaccgt
     1561 ggatgcgcgc cggcttgtcg gccgcggcgc ggcccatcag atccggcagc gcttcaatca
     1621 cacgctcagt caactcgaca tcgcgcttat ccagctcgcc acgtttcaca ccaaggttga
     1681 ccatcgggcg cagacgtcca tcagcgaaat agctgatcgg gtcggtccac tcgccggtat
     1741 ccattttcac cgggtcctgc aatggtccga aatagcatgt gccgttatat ccgtcaggcg
     1801 cagacccgaa atacttggca cccgcatcat gcatacgggc cagagccgcg ccaaaagctc
     1861 gtgccgcctg aggcgtgggg gacgcgctac ccacacgttc gatatccaga taatccttgc
     1921 cccagccgta tacctgcacc acgcgcgggc cgccttgcgc atgagccgct cccaaccatt
     1981 cgagtcctcg accctcgcat tcgaagaatc cttccggtgc gaatgccctg cttttgcgat
     2041 acgtggccat aatgctccct tcgtcgctta cgtacnnnnn nnnnntggat gttaccgatt
     2101 cttagtgttg ttctacaccc ccttgggtag ggtgttcatc gtcataacac atcaaatact
     2161 tagaaatcac gaggggttat gaattttttc caagcgatca ttctcggcat cgttcaggcg
     2221 ctgaccgaat atctcccggt gtcctccagc gcgcatatcc gtattttcgg cgatttgatg
     2281 ctgggctctg atcccggtgc ggcgtttacc gccatcatcc aaatcggcac cgaacttgcg
     2341 gtgattctgt atttccgtca cgacatcatc aatatcctta ctcactggtt cagctgcctg
     2401 ttcggcannn nnnnnnncgg cgacaattac gccacactcg gctggaacat tatcgtcggc
     2461 tcgattccga ttattatcct cggcttcact ctgcagaacg tcatcgaaac ctcgttgcgc
     2521 aacctgtgga ttacggtaac cgtgctgttg gtattcggca ttctgctgtg gatggtggat
     2581 gccaaggctc gccagaacaa aaccatgaat gatatgacgt atcgtgatgc attcctcttc
     2641 ggtttgggcc agtccatggc gctgattcct ggtgtgtccc gctccggcgg caccatcacc
     2701 gtcggtcgcg cactgggcta cacgcgtgag gccgctgtgc gtctgagctt cctgatggcc
     2761 atccccgcgg tgttcggctc aggtttgctc gaagccatca aggccgtgaa gaactacaaa
     2821 accgatgcga tgttccccgg ctgggggccg accctcgtag ccatggtcat cagcttcgta
     2881 ctcggctaca tcgtgatcat cggattcctg aagttcgtat cgaacttctc ctataaggcc
     2941 ttcgccatct accgcatcgg tttggctgtg gtcgtcgcct tgctgctgat tgtgggcgtg
     3001 ctgccggcca tcgatccctc cgtggtggct gcggcataag acgggtcgat aggcgactca
     3061 atattaatgg tgctcccgcc ggcgggtggt cactggaata tgccagcaga accaccctca
     3121 gtctcgctac gcgagccagc tcccgtcagc gggatattgt gtatataggg ctaattgccc
     3181 atctggcgta gaaacgcttc cgcttctttg actagttgtt cagcctgggg cgctcccaat
     3241 gaagcctccg cctcggcttt ctgctgcaag tcgtagtcgt gctccaaatg ctccacataa
     3301 tcatgaagct ggtcattgca acgaaccagt atcgatgcct gcgccttcca ctgttcggct
     3361 ttctgcttca ggtctgccgt atcgaagatg aagccgatat acttgcccaa cgcatccagc
     3421 aatcgtatag tgccggcaga gcattcgtcg ctgcccaaat attgggggat ggacacccac
     3481 atcgaggaat gagcaaatcc ttgttgtgcg gcagctacat ccagaacagt aggaatgccc
     3541 accgggccgt tatacgaccg gtctccttcg cattgacagt ctccgtcgct gacggcgatg
     3601 ggcaacggac gggtatgcgg gcaatcggag aacatcgaac ctaacgtaat gatccgattg
     3661 acatccagct catcggcaat ggccagactc tggctacagt attccttcca ccgatagttt
     3721 ggttcggggg cgatctgcgc atagatacgc ttgcctgcgt ccaacgtgat gtcgtagaac
     3781 gtggtctgtg gccagataag attggtgcga cccgaaacat ggcacagcat aggacgggca
     3841 acctgatagt catagaaatc atcacattta atatgacgaa tctcgcggga ctcatatcgc
     3901 ttgaccagat ggcgcaccac gttcgtcgcc gcctgacagg catcattcca tccctcgaat
     3961 gcggcgatca atactgcgcc ggtgtccttc gctgcttcac tcatacctct agagtaactg
     4021 gagttcattg cccgaagcgc acgctttagc cataagcgag agtatgcaat tacaggcgac
     4081 acgccgcagc tggacgaact acacgcatgt gtgtatagta aacactcgtg cttcggcagg
     4141 gagacctcgc caaggaaaca cacgcggaca tagcttagtt ggtaaagcgc aaccttgcca
     4201 aggttgagac cgcgggttcg agtcccgttg tccgctctaa ggttccgatt gattatcgac
     4261 ctttagggtg ggttagccaa gcggttaggc agcggcctgc aaagccgtat agacgagttc
     4321 gactctcgta cccacctctc agaccagcga agcaattcaa tgggatgaaa tatgacccgg
     4381 gcggttggcg cagtggtagc gcacttccct gacacggaag gggtca
//
LOCUS       DAWBMW010000128         4402 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_4205_length_4402_cov_2.613527, whole genome shotgun sequence.
ACCESSION   DAWBMW010000128 DAWBMW010000000
VERSION     DAWBMW010000128.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 4402)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4402)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4402
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4205_length_4402_cov_2.613527"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    760..769
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          994 a         1256 c         1288 g          854 t
ORIGIN      
        1 gcatgttcgt gcacgtttcc ctagtgccgt acatttccgc cgcccacgag ctcaagacca
       61 agcccaccca gcactccgtg atgatgctgc gccagctcgg catttccccg gacgcccttg
      121 tgctgcgctc cgaccgcccg ctcaatcagt ccatcaagga caagatctcc ctgatgtgcg
      181 atgtcgatgc cgaaggcgtg gtcaactgcg tggatgcgcc gagcatctac gatgtgccga
      241 agatcctgtt cgaggagggc ctcgacgcct atgtggtgcg tgagctcggc ctgccgttcc
      301 atgacgtcga ttgggacgaa tgggccgacc tgctggagcg tgtgcaccac cccaagcacg
      361 aggtcaacat cgccatcgtc ggcaagtaca tcgatctgcc ggacgcctac ctgtctgtca
      421 ccgaggccat caaggccggc ggctttgcca actgggccaa ggtcaacgtc aagtgggttg
      481 cggccgatcg ttgcgaaacc accgaaggtg cggccgccgc tctcgacaac gtcgacggca
      541 tcgtgatccc cggcggcttc ggcatccgcg gcatcgacgg caagatcggc gctctgaagt
      601 tcgcccgtga aaccaagctg cctgccctcg gcctgtgcct gggcctgcag tccatggtca
      661 tcgaatactc ccgccacgtg ctgggcattg aagacgcgaa ctcttccgag ttcgagcccg
      721 attgcgccaa cccggtgatc gccaccatgg aagagcagan nnnnnnnnnt gggccacacc
      781 atgcgtcttg gctcctaccc ggctgagctg gaggaaggct cgctggtcgc cgaactgtac
      841 ggcaccacgc atgtcaccga gcgtcaccgc caccgttacg aagtcaacgt ggcctacaag
      901 gatcgtctgc gcgaaggcgg cctgcgcatc tccggccaga gcccggacgg cgagctcacc
      961 gagttcgtgg aactgccgca ggacgtgcac ccgttctatg tggccaccca ggctcacccg
     1021 gagttcaagt ctcgcccgac caagccgcac ccgctgttcg ccggcctggt caaggccgcg
     1081 ctcgaccatc aggcggcgcg ctgacgcact ttcgtgccgt gcataatatc gcggcatgat
     1141 gtgctggctg ccgtcatcgg cgtagtgcaa gacatacggt aaagacgtgg gagcgaaata
     1201 tctaatgatg tttcgccacc acgtcttttt tgttgtatga gcagcatgca gcatgatgta
     1261 ttggaaggcg acggtaaaaa attaccgttt ctatggtgtg ttggtagtat gcagataacg
     1321 cttagtaggc catcgcagaa agaccgacca tgaccgaaca ccttgatgac tcgacccagc
     1381 agtttgcgcc tctgtttggt gcgaataatg ccgtcagcgg cactgtggca gacagtgtgg
     1441 atgctgctgc atcagattcc attcagggcg atgagatgag gaggcgccgc atctggccgt
     1501 gggtggtgat tgcttgtctg ctggttgcct tgggggctgc ctgtggcggc ggtgtttggt
     1561 tcttccaaga tcacgtgctg cctggcacaa cgctatgggg aaattcagtt accggcaaaa
     1621 ccgagcagga gattgtcgac ctaatcaacg accaggtcga caacaccgcg gtacgagcga
     1681 aataccaggg gcagtcggcg acgttcacgc tgaaagatct cggcatcgaa gttgatggtg
     1741 aagcaatcgc atccgatgtc atcaatgccc aacgcggtga cgcatggtgg caacagtatg
     1801 cgttctggat tacgcatgat gtggccccgc aaatcaatcc cgagctcgct gatggcagtg
     1861 tggtagataa ggcgctgaac attgatgcac aagaacctgt tgacgcgcaa gtcgcattga
     1921 acgataacaa cagtggcttc aacgttattg cgggagaaaa tggccaggga gccaacgcca
     1981 ccagcattgc gaagcaagcc atttccacag tggagtcatt gggcagtgtc cagccacaga
     2041 ccgttcgggt ggaactcgac gtaaccgaac cgacgattac caatgcaatt gccgatgagg
     2101 cgaaaaccac gctggaaacc ctagtgaaca atgccgtggc catcaaggtg gcggataaag
     2161 acatcgccac cgtgaatgcg tccatgctgg gttcggccat gcgcatcgac gccaaccagc
     2221 agtcaaagct gaaagacaat gaagtacgta atggctacgt ggtctttgat gccgataagc
     2281 tgcagcaata ctatgacgag caaatcaagc cgaatctcaa attggaacgt gaagacaaga
     2341 aggtcgtcgt caataatgct ggcgaggtgc tgagcactga aacagaaggc catgatggca
     2401 ttgtgattgc cgatggcgcg gatacggagg ttggtgctcg cttggctcag gtgctggcca
     2461 cgggaactgg ctcagtgaat gtggacggca aggtagatcc caaacaggaa gtcaagatca
     2521 ctcatcgcgt ggcgattgac ctgtccgacc gtaagctcta tgcctatgag aatgacacgg
     2581 tggtcaagac attgaatgtc gtggtcgcgg agggcaacga caacgtcacc ggcgaatgcg
     2641 ttggcatgat gtgtacgccg acaggtgact tcaacgtatg gcaaaagttg cccagtcaag
     2701 atatgagcgg cacgctgaat cttgccgatg gcacggtgga aacatgggat gtcaaagacg
     2761 tgggcttcgt caattacttc tcctccggtt gcgcaatcca ccgtattgcc ggcggatatg
     2821 tagcggatgc cgtaatgccc tccattgcca acggatctca tggttgcgtg ggtatcggat
     2881 gggatgtggc cgaatggttc tacaactggt gcaatatggg cacgagcgtg catatccaag
     2941 tgtgatatgc aacagtgtga tatgtaacaa atacggcatg cagtggtgcg acacataatg
     3001 ccgcatacgc gatgttcggt gtgcatggga caagcttgcc cggctccaat atcgctgtat
     3061 cgcattcgtg taatcaagcc cacagccaat atgcgtcagg gcggctagtg tttgcacttg
     3121 ggttctgcta ggttttcata ggattaagtg tgatacaagg aaggcaggat ggtgaccgaa
     3181 aacacagcac caaacgccgc cgctgacgtg gagatgagcc agtatgtggc tgatcgtacc
     3241 cgcgtcaacg aggacaagat caagaaggat gacgagatca tcagccagtt cggcgactac
     3301 aactatggtt ggcatgattc tgatgccgcc ggcgaagccg caaaacgagg cattgatgag
     3361 aacgtcgtac gcgcgatcag cgccgacaaa ggcgagccgc agtggatgct cgacatgcgt
     3421 ctgcgtggat tcaaggcgtt cctcgaaaag cccatgcccg attggggtgt ggatctctcc
     3481 ggcttcaacg cagacgactt caagtactac gtcaagccaa tcgacaagca ggcgaaaagc
     3541 tgggacgaac tgcctgacga catccgcaac acctacgatc gtctcggcat cccggaggcg
     3601 gagaagaacc gcctcgtctc cggcgtggcc gcccagtacg agtcggaggt catctacaac
     3661 tccatccagg aggacctgaa gaagcagggc gtcatcttcg tcgacacgga caccgctgtg
     3721 cgcgaatacc ctgaactcgt caagaagtac ttcggcaccg tcgtgcctcc ggaagacaac
     3781 aagttcggtg ccctcaacac cgcggcatgg tccggcggct cgttcgtata cgtgccgaag
     3841 ggcgtgcatg tggacatccc gttgcaggcg tacttccgca tcaacacccc ggccatgggt
     3901 cagttcgagc gcacgctcat catcgcggac gaaggctcct acgtgcacta cgtggaaggc
     3961 tgcaccgccc cgatctggtc cgaggactcg ctgcacgccg ccatcgtcga gatcatcgtc
     4021 gaaaagcacg cccgcgtgcg ttacaccacc gtgcagaact ggtcgaacaa cgtctacaac
     4081 ctcgtcaccc agcgtgccta cgtgcgcgag ggcggcacga tggaatgggt cgacggcaaa
     4141 atcggttcca aggccaccat gaagtacccg gcctgcatcc tcgccgaacc gtacgccaag
     4201 gcatccacca tgtccctcgg cttcgccggc aagggccagt accaggacac gggcgcgaag
     4261 atgattcacc tcgccccgca caccagctcc accatcgtcg ccaagtccat ttcccgtggt
     4321 ggtggccgct ccgcctaccg cggtctggtc aaaatcgtca agggcgcgta cggttcgtcc
     4381 aactcgaccg tgtgcgatgc cc
//
LOCUS       DAWBMW010000129         4373 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_4240_length_4373_cov_2.434460, whole genome shotgun sequence.
ACCESSION   DAWBMW010000129 DAWBMW010000000
VERSION     DAWBMW010000129.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 4373)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4373)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4373
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4240_length_4373_cov_2.434460"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          862 a         1321 c         1286 g          904 t
ORIGIN      
        1 acttcgaagt ggtcgagctg ctgcttgacg attttggcca cggcctgagg agtggagacc
       61 tcgggagtga gaatcttcaa tcgtggctcg gcgaaatcgc ccttggtcag atcgacaggt
      121 gcggccttga tgccggaacc gccaatgtca acaccgaaag cctgtgcagt ttcaatcatc
      181 gttgaccttc ctttccatac gttttcatca gttcgtaagg ttgcgcccag tttaacgcga
      241 attacgctca acacgaaacc attacacacc aattgtgcaa acgtgtgcat cgtcgccggt
      301 cactacgtac cctattcgta tcgtttttca agcctgacaa tgggcaatgc atgcgatttc
      361 gcatactagg aaaaatcggc gtctatggtg gcctgactct gtgtcgcgcg cgataatggg
      421 aggtatggca gaacataccc aacttattga tgcaattaac atccgcaccg ccgtacgcgc
      481 ctacgatgat gagccgatcg acgacgatac ggcccgccag ctcgagatgg cgcttcagcc
      541 gatcaactta ctcggcgatc tcaacattca gctggtgcgc gaccagccca aggtgttcgc
      601 cgaagccaat gcttcaggtc atctgaccaa tgcggcgaat tatctggcca tcgtcggccc
      661 cgcgaacgat gaggaagcca aagagcgtgc cggcttctat gccgaacgca tggtgctcac
      721 ggccacgttg cgcggcctcg gcaccttatg ggtcgccggc tcatgggaca aggcggaggc
      781 ggccaagcat tgccgcgtca cctctggcca ggagctgtat ctcggtgtgg tgatcgggca
      841 tccgaagaat catctggatt atcaggcgaa atcctatgag gagctatgcg aagcgcaacg
      901 cacgcaccgc gccaccaaga cctatgagca gttcaccgcc acaatgagcg atgaggggcg
      961 cgaggccgct ccggattggt tcaagtccgg cgtcgaggct gcgatgaagg cgccgagcgc
     1021 gatgaaccgt cagccaatca ccttctcgta taatccggcc gacgataccg ccgccgcgca
     1081 tatcgatcag agtgcggagg atgagcatca cgcgttcaat gacatgggca tcgccaagct
     1141 gcacttccag atcggtgccg gccaaggcca gtgggcttgg ggcgacggtg gcctgttcat
     1201 ccataagtaa cctgtagtga cccccctcat agaggggagc cactactacg gctacttcag
     1261 cgacggcatg ggcttccagg tggggtcgtg catcagcttg cgcgagtccc accagccctt
     1321 ggcaatctgt accaaaccgg tcttgaggtg ctcgcgatcc accatcacca ggcgaatgac
     1381 ttccttggcc gcggtgagca cggtacccag tccaaacacc agcggacgga agtcaccata
     1441 ggtcatgaag taccgggcca tgtatccgcg gttgcgcatg atgtgatacc ggttcatgtc
     1501 ggatgtggag ttgagctggc gcacgccggc gatatcccaa ttgccgattt cgcgcgtacg
     1561 gcgcaggatc acgtcgggca ccacaatcgg gttggtgacc ttgctggcgc ggtagccata
     1621 catcgtgtca tcccagtaga tgaagaagcg cgggtccggc agaccgattt ccgtaaccac
     1681 acggcggttg aacaggccgc cttcgaagca cagcgtgtcc atcactcggt atccggccgg
     1741 gccgaatgcg gcgggcgcaa tcgggttcgg gatgcccagc ggcacaatga agtcgtactg
     1801 ccagtagaac gggccgccat cgtagtcgaa tcgggagccc tgaatcacct cgtgcttgtc
     1861 ggtccacttg gacagcttgg caatggcgtc cggcagcacg gccacatcgt catccatcac
     1921 ccagaaccat gcggcaccca gctcgtaggc tttggccacg ccggcggaga aaccgcccgc
     1981 accacccagg ttctcggtct gcggcgcata gaccacacgt tcttcgttac cggactggtc
     2041 ggccacggtg gtgccccatt ggccggtcac cttgccggca aacgcagcga ccatgtccgc
     2101 cgtctgatcg gactgctcat tgtccacgat gacaatgcgc cacggtgcct gttcaagcgc
     2161 cagaatcgaa tcgaacagtg tctcaagcaa ctgctgacgt ttgtaggtgg tgacgatcag
     2221 ggcaagcttg tcgatggtgt tgttgggttt cttcacttca gccatggctc ccattgttgc
     2281 acttgaccgg cacaagacga atttctgcac agtgaaacgc gggttgcata cgtccggcgc
     2341 atggcgtatg aataagcctg tcagtttcag ggaaggtgca attccttact ggcggtaacc
     2401 tgcaccccgg gtttcataac ggggatggca ggaagcccgc gacccgcgca agcggctgat
     2461 ccggtgagat tccggagcca acggtatagt ccggatggaa gaaacgaggg ctcattatga
     2521 gtgtatcttc gcgtattcgc atgcaagttg ttcgttctgt ggcacccgag gccgagggtt
     2581 cggccagcac gtccaccgcc agcaccagcg ccaaacttgg ctcacacacc actggtgtgg
     2641 ccgattctgg ccgctggtcc acgcgccgta tcgccatgta tgcactgttc gtggcgctgg
     2701 cgatggtcac cagcttcatt gaattcccga ttacgccggt cacttggctg aagtacgacc
     2761 cgtccggcat cgtctgcctg attgccggct ttgcgtatgg cccggccgcc gccgcaatcg
     2821 tgtctgtgct cggctttgtg ccgcacatgt tcgccaatcc gtggggttcg ctgatggctg
     2881 tgctggtggc gctgttcctg tctgtgccgg ccgcgttcat ctaccgtaag atccgtacgc
     2941 gcaagggtgc cgccatcggc attctggtgg gtgcggtgct ggccatcgtg gtggcgctgg
     3001 tgagcaacct gattgtcacg ccgatttacg cgcacatgac ctaccaagct gtggccgcga
     3061 tgattctgcc gattctgctg ccgttcaacg tggtcacctt cctgatctac aagccgatct
     3121 ccaacctgct gtccaagcag cagtgactgg cctgagacgg gccgatgccc gccacgacta
     3181 acgaccactc aaagaccact caaagaacca gaacgcagaa tcccggcacg ggccgggatt
     3241 ctgcgttttc gcctatcagt tacggattgc tgtttcatga ccgttgcacc ccattccttc
     3301 gacgccgacg agttccacga ttctgccgaa cctcacgctt ccgccgagcc tgcctccccc
     3361 gccgccgtgc tcaaggacat tcgtttcagc tatgaccgcg tcacctcatg ggctttggat
     3421 ggcgtttcac tcacggtcca tgccggcgag cgcctatgct tggtcggtcc gaacgggtcg
     3481 ggcaaatcca cgttagcccg tctgattgcg ggattgaccg cgccggacgg tggtgaagtg
     3541 acgttactgg ggcaacgcgt gtacgcagcc ggcccgaatg ccgatgccta tcgtgctgcc
     3601 cgccacggca tcggtatggt gttccagaat cccgaggatc agctggtcac caccgtgttg
     3661 gaggatgacg tggcctttgg cccggagaat ctcgggctgg agcgcgagct catcggcgag
     3721 cgtatcgttg attcattgca ggccgtgggg ttggcgaact tacgccagtc cgacccgacc
     3781 cgtatgagcg gcgggcagca gcaacgcgcc tccatcgccg gcatgctggc catgaacccg
     3841 gcaatgctgg tattggatga gccgaccgcc atgctggacg aatccgcacg cgccgaagtt
     3901 atgcgcattt tggatgattt gcaggcgcgc ggcaccacta ttgtgcatgt cacgcatcat
     3961 ccagatgaaa ccgttcatgc cgatcgcatc gtgcacatgg aggccgggcg aatcattggt
     4021 atcactgcag ccgtcgataa tcgatccccg ctagcggagg ctgtctcgca aagcgagact
     4081 gagggtagta tcggcacaga agccgccccc agtcgcccta cgaacgacag cccccgccag
     4141 cgggagcgcg aagacggctc cgaactgccg ctgctctccg ctggcatcgg tgatatgacg
     4201 aaccccatta ttcgcgtctc gcatctgacc tatcgctatc cttcggccaa acgggcggtt
     4261 atcgatgacc tgtcgttcac catcgcgcgc ggtgaaaccg tggcactgat gggcgtgaat
     4321 ggttcgggca agtcgacgct ggtgcgcatg ttgtgcgcac tgactgcgcc gac
//
LOCUS       DAWBMW010000130         4364 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_4252_length_4364_cov_2.313298, whole genome shotgun sequence.
ACCESSION   DAWBMW010000130 DAWBMW010000000
VERSION     DAWBMW010000130.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4364)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4252_length_4364_cov_2.313298"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    717..726
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1252..1335
                     /estimated_length=84
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          836 a         1362 c         1228 g          844 t
ORIGIN      
        1 gctcatggaa ggctacatcg gcaaatacag cctgatggag cacgacggtg accttgacgg
       61 catgaccgac acgctcatcc gcatcaccga gcgtgtggcc gaggtccgcc gcctctacca
      121 gccggacttc ccgctgccca ccttcgccga aattcgtcgc gtggtccagg acgaatggga
      181 tgaagacatg ggcaagatcg atcccaggga agacaatccc accgccggcg aaatcgaaga
      241 ggaaaccgaa tcagccgatg acgccgccgg cgagggcgac caggcatgag tggtgttacc
      301 gacagccaca aaacggctgc gtcagttcag tccgaaaaat cagtggaatc cgccgagccc
      361 gccgaaatcg ttgccgtcac ccaaggcgaa accgaacggc gcatgtctga cctgtccgcg
      421 ccggccgggg ccagcagcca tggcaaaggc aggctttccg cacgcggcaa ctggcatacg
      481 cgcctgcgcg tcggcatcat gggcggcacg ttcgacccga ttcacaacgg ccacttggtc
      541 gccgcctccg aagtagcttg ggtatacgac ctcgatgagg tgattttcgt acccaccggc
      601 cgacctgtgt tcaagctgga caagcacgtc accaacgccg aagatcggta tctgatgact
      661 gtgatcgcca ccgcatccaa ccccaaattc accgtctcgc gtgtcgacat cgaccgnnnn
      721 nnnnnngcgg gatatccgcg ccctacatcc cgacgccgag ctgttcttca tcaccggcgc
      781 agacgccgtg gccgaaatta tgcagtggaa ggacgccgac ctcatgtggg acctcgccca
      841 cttcgtggcc gtcacgcgcc ccggctactc cagtccggac ggcgtgacct tgcccgaagg
      901 aaaggtcgat acgcttgaga tcccggcctt ggccatctct tcgaccgatg tacgccgccg
      961 cgccgaacac gacgagccgg tctggtatct ggtgcctgac ggcgtggtgc aatacatcgg
     1021 caagcatggt ctgtattcat acgaggactg acgattcgta cgaggactag tgtgcatttg
     1081 tacgagggct ggtgaggatt agcgcgaatc agtgcgcatc aatcctcacc agtgcaaagg
     1141 ataagcagtc aggccagtcc catcttgatt ttcaatgcca ttacgcgagt cgccgccgcc
     1201 gtcaccttcg aagcgaaagc cggatcggac ttcgcccgtt cgttgaggcc gnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnccccc cgcctcgaca agctttactc ccaattggct tacatcgtag
     1381 ctgctcaacg cttcggcaga catcgaatcc gaaatcacca cgcccgtgta cttcagggcg
     1441 ttgcgaatat gaccgtcgat aatcgtagaa gaaaacaccg ccggattatt cgggtcgatg
     1501 gattgatacg tggccaacga catcatcacc atcgcaggat cggtcttggt gatcgcctgg
     1561 tcaaaggcac ctgcctcggc cccgcccaac gtggtggtcg tatccagaat gccttcggtg
     1621 gtgaagtcgg tattgccgct taccgcgccc aacccaggat aatgtttgac cgccgcgccg
     1681 acctgagcgt ctcgcaaacc ttcaataacg gcgatgccat gttcggcgtt gccggccgca
     1741 tccaatccga aatcacgatc cagcgcgcca atcggagcat ttgaggcacg atcgcccacc
     1801 accgtgccga gcaccggtgc caaatcgaca tttatgccag cagcagctaa ttgcgaaccc
     1861 cacgtgcctg ccgattgacg caatgcgtca gcggacattg tgccctgctc aacggcactg
     1921 ggcattgtgt cgaaaccggt accagtcaga tgctgcacct gaccaccctc ctgatcggtg
     1981 gcgatgatga gacgattgcc ggccggcgca tatccttgga gttgcgcggt cgcggtcttg
     2041 accgtatcgg cgccggtatt ccaattgcca atgagaatgg ccgagccaat gtgttcgtcg
     2101 gcgattgttg aagccaacga ggagggatcg gaaccggcaa gcaacggcac catcactaac
     2161 tgtcccacac gttcctcaag cgtcatggcg gataccgcac gttgggcttg tgcggccggt
     2221 tcgttcttct tcggggaagg aacgtcggcg cgtggtaacg cacttgtctg ggaggtctgg
     2281 ggagtggtgg tgttgccgtt tgatgtggat ttggcgaacc atccgacatg ccatccgtag
     2341 gctgccacgc cggccaacac caccgccaaa gcacatattg tcgccacgat tggagtgctc
     2401 ctgtgccgaa gttggcgtgt ggcgtgtgct gagggacgca tatgttgtgg ccgttggctg
     2461 ttcataccgg caagcgtaga acggagcgta gaacagaacg ctgcctcatt ctgtgctcag
     2521 ggcataacct acctcccgcc ctttcgatag gcttgagtta tgtcttttaa cgatcctttc
     2581 tcagtactgt tcggcaatgg cggcgattca cgacgcaaca atgcgaacaa tgacgatccg
     2641 atcatcctcg atgtcgaggc cgatggggac ggtccggccc gcaacaccaa cgccggcccc
     2701 tccgggtcat cccgccctcc acatggcccg gccaatccgc gtatcacccg taagccagcg
     2761 tccggtggct caggaggcag ccgtggcagc aagattctga tcggcgtggt gctggccttg
     2821 gccatcatcg tcggtctgtt cttcggcctg tcccgattca tcaccgatct gatgtggtac
     2881 ggccagctcg gcttccagtc cgtggtctgg acccagctcg gggtgaaaat cggtctgtgg
     2941 gtggcctacg cgctgctcat ggccttgacc ggcttcgttt ccgcctggct ggccattcga
     3001 gcacgccctg attcggccga cggttccacc attcgcatta acggcgatgt ggtcgaggtc
     3061 ggcaaatccg tcagctcgaa aaccgctcgc cgcgtggccg tggtgatttc gctgatcgtc
     3121 ggcgtcatct tcggctctca gttcaacgcg aactggagcg agattctgct catgttcaac
     3181 gcgcagtcgt tcggcaccac cgatccgcag ttcggtctgg acaacggctt ctacgtgttc
     3241 gtgctgccgg gcctgaagct cgtgctcgcc gccgtggcca tgctgctggg cgtgggcctt
     3301 gtgttttccg tggtcactca tgtgctgatg ggtggtatcc gcattaccat gccggttaac
     3361 ggacgcggcc tgttctccat taccaagcgt tgccgtcgcc agcttggcat ctggctgatg
     3421 ctcaacatgt tcgcttgggc agtgcgccag atgattggcg tcttcgacca gctcaccgtg
     3481 cagggttcac gcatcaccgg tgcctcctac accgccgtac atgccaacat ccccgtcacc
     3541 ttcatcatgg ccgtgctgac cgccatcctc ggcgtggtgc tgggcatctg gctcatgcgc
     3601 tcccacacgc tcgaaggcca ggcgtccatc ggcgtgcgcg cttccgccgc gctcaaggcc
     3661 tggcgtgtgc cggtgacctc cattgccgtc gtggtcgtgg tgggtcttgt gctcaccgtg
     3721 gcatggccgg tgctgttgca acgcttccgc gtcaacccga acgcacagga gatggaatcc
     3781 acctacatcc agcgcaacat cgacgccacc cgcgccgcct acggtcttga caagctcaag
     3841 accgagcaat acaaggtgac cgacaagggt gagcagggcg ctctggccaa ggaggccgac
     3901 accaccgcgc agatccgtct gctcgacccg caggtcgtca gccccacgtt caaacagttg
     3961 cagcagtcca agcagtacta caccttcgcc gacaccctgg cggtcgacaa gtacgagatt
     4021 gacggcgtct cccaggacac cgtgattgcc gcccgcgagc tcgatctggc cggcaatgac
     4081 aaccgcaact gggtcaatga ccacaccgtg tacacccacg gttacggcgt ggtggccgcc
     4141 tacggcaaca aggtgaccgc cgacggccag cccgaattct tcgaatccgg catccccacc
     4201 cagggcaagc tcaccgaatc cgaaaagtac gagcctcgca tctacttctc gccgaacacc
     4261 accgaatact cgattgtcgg tgcccctgaa ggcacgcagg cttgggagtt cgactacccg
     4321 accggttcgg aaggtgctct gaccacgttc aagggcgacg gcgg
//
LOCUS       DAWBMW010000131         4359 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_4259_length_4359_cov_2.832946, whole genome shotgun sequence.
ACCESSION   DAWBMW010000131 DAWBMW010000000
VERSION     DAWBMW010000131.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 4359)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4359)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4359
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4259_length_4359_cov_2.832946"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2496..2505
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          961 a         1304 c         1144 g          940 t
ORIGIN      
        1 ctgcgccacg gtattcgaag ctacggcgtt cagcggtttg ctgctcgatt cgtgcgaata
       61 cccccagctc gtcgtccgac agctcacgca cgtgcacacc catcgattgg gcacgcttca
      121 cgctccactg ggtgcgcttg tcataggact tcagcagact tttctcatcg gtaatgccgg
      181 tcagatcctt gaggtagcgc caacgcggaa ccgccgtgta gcccacggtg aaaccgccat
      241 gagtgaagcc gatcgcctcg agctgttcta tcagcttatt gtcgggagcg ccattctggt
      301 catccggcaa agaagctcca ttggtgtcgc gaagccgata cggggattca ggggtgattt
      361 ccagctgtga agcacccttt gccttggcat ggcgtttcag cgcatccatg aagaaggtca
      421 atgcttcggt gtcgtgatag tcgcacatcg ggccatcatg gatgaccgca aatgtagaga
      481 atcgagagcg gtgagtttcg aacagtgccg ccgcgacgat cttctcgccc tccttgaggg
      541 ccaaatattc gacgtcgatc ccctgcgcgg cgcgaaggcg tcccatcgcc gaggtttgtt
      601 ggaaattgcc ttgtggatgc ctggcggaga aatcatcgaa ctcctcgtct gtcaacttca
      661 ccagcacaaa atcacgcatg cttccctttg cctccgagtc aatcaggctc accatgctag
      721 catagcgcgc acacgacgaa tggcggccga gcagatgagg tgtgcacatc gttcgcacta
      781 ttgccgccac gatggtccaa agaacaatgg cccaaagatg atgaatgtcc ggcttgcagt
      841 gaatcaaacg cagtgctata ttctctactt ggctcttgaa gccacgccac cttagctcag
      901 tggtagagca tccccctcgt tcgtgggaag gtcggtagtt cgattctatc gggtggctct
      961 tgttcatcaa gaaggccgac gcactaagcg ccggccttct ttcgtatcgc ccttgctagg
     1021 tcataggcgc ttgctagaca ctcgcccgat tcgggggctg agtgctgagc cgcgactcaa
     1081 ctgtggctta gtcgcggctc agtgcagcta gacagcctag acgacttagt gcagcagggc
     1141 ctcgatggcg gggaccaagg ctttcagcgc cttgcctcgg tgggaaatcg cattcttctc
     1201 ggccggagtc atctcggcgc tggtcagcgg ctcgccttca tggtcaggct cagtgctgac
     1261 gcgaccggca ggctgatcgt ccgggacaaa cagcggatcg taaccgaaac cgtgtacgcc
     1321 acgagcctcg cggatgatgc gacccggcat gtcgccgagc tccactgtct ccgacttgat
     1381 ggcgtatcgg gcatgcaccg gggcagggct cccagcggca gccgtggtgt gaacggtgat
     1441 gccgtcggca gccactccac cggtcacgtc ggcaccggtc tcggtgtccg ggaccaccag
     1501 cgcggcggcg cagcggaagc gcgcggtacg cttgtcatcc ggaatatctt cgatttgggc
     1561 gagcagcagc gcattattgg ccttgtcgtg gccgtgagca cccgcccagc gagcagacag
     1621 aatgcccgga gcgttaccca tcacatcgac gatgagacct gaatcatccg cgacagcggg
     1681 cagaccggta cggatggcca cagcgcgggc cttgagtagc gcgttctcct ggaaggtgac
     1741 gccggtttcc accggatccg gcaggtgaag gctgccagcg gacaccagtt cgatgttttc
     1801 ggcatccgcg ccaagatcct cttcgagaat gcggcggatt tccacaagct tgccttcgtt
     1861 atgggttgcg acgacgattt gtatgaggtt ctcctttaag aaggctcccc ttgtcagggg
     1921 agctggctta gtctagggac agagcggcac gctgggcggc ttgcagttcc ttgttgccct
     1981 tttcggccaa gtcaagcagg gtgccgagtt cggcacggtt gaacggacgg tgttccgcgg
     2041 tgccctgaat ctcgatgaag gtgccggaac cggtcatggc cacgttcatg tcggtcatgg
     2101 cctgactgtc ttcaatgtat ggcaggtcaa gcataggagt gccgttgatc acgcccaccg
     2161 atacggcgga cacatagtcc ttaagcacgc ggctggcgga cttgatatgg cggtgcttct
     2221 ccgcccaatt aactgcgtcg accaaagcca cgtacgcacc ggttacggaa gccgtgcgag
     2281 taccaccatc ggcctgaagc acatcgcagt caagctgaat ctgattttcg ccgagcgcct
     2341 tcatatcgat gacgccacgc aggcagcggc caatcagtcg gctgatttcg tgggtacggc
     2401 caccgatttt gccacgtacg gattcacggt cggtgcgttc ggcggtggca cgcggcagca
     2461 tcgagtattc ggcggtgacc cagccgaggc cggagnnnnn nnnnnccttg cgccaacgcg
     2521 gcacaccggg agtaaaagtg gcggtgcaca tcacgcgcgt gttaccgcat tcgatgagca
     2581 cggagccttc gggggcgtcg gtgaagtggc gggtgatgcg cacagggcgc agttcgtcga
     2641 tcttgcggcc gtcggcacga attatttggt gatcgccaag catgtctttg atttcaacca
     2701 tgcgccccag catacgccat ccgctctatg cctggatagc aactcgtcaa ccatcatcga
     2761 actccagcgc aatgtcattc cctgcttaca tatccatccc atgacacttg gctgcacgcc
     2821 actattttct tatgcaccac tcgcttacag agtcaatgcc tgcgtacctg atgaggattg
     2881 ggaaagatcg tccgctggaa taaggatgcc atattccatg aaagcttggt tgaattgctt
     2941 gcgctgcata gcgttgagtt cgtaatcgat taacggcaaa tatgtatagt ccatgtcatg
     3001 caacggctct tcatatcgat gaagcacgtg cataattgcc gcgctcggca gtggggacca
     3061 ccatccatcc aagccatacc ggtacggagg aaccaatgtc aagcgtctgt tccttgggtt
     3121 gatatcccaa cgcagaaaac ggtataaacc agcgagaaac gcaatccagc acgcatcgtt
     3181 cacccgtgca accgctcgga cgaacgatat acgttgatgt tgaacattcc gagcatgaat
     3241 cagattgacg cagagcatct tcaacgtaga ccaggtcgct ccgctttcta ttgcatcgaa
     3301 atcaggaggc ctacgcagtt gcgccaacaa catgtgcaat tcgtcttccg gaatcgtttg
     3361 cgagggggcc acggcttcta ggtgaagacc aaaaccagcc cgctcaagcc cctcgaccaa
     3421 aacatcaaac aacatacctc tttgcccacg caatacgcga ccaattgcag accgatccaa
     3481 accagtgcgc tgtgcaagct cataggcatt ccaaccagtc tgcgacatca cttcattcag
     3541 cacagccgca cacgaattaa cagggcaacc agataccaca ccgtgatgca cgacatcgca
     3601 ctgttctaac gctcgatgct ctacagcgcg ccgctctgat acgtcatcgt tgcccgccgc
     3661 gttctcatca ttgagagttt cactcatcga agtcaccttg cctcggccga tttcccggtc
     3721 atttcatcaa accgcaatca tcatatccca caatcccatg acgatacgat tcgtcatatc
     3781 ccacaattca gagacggtat gattcgtatc gcatcagttc acacgattct tcaacgattg
     3841 ctccgcaaac gattgctctg cacgcatcac ggcattctac cggcacacta ttcctcccaa
     3901 tcagcccaac gcaacatcac acgcaacatc acacgcaaca tccctcacgc aacacccctc
     3961 acgcaacacc cctcacgcaa cacccctcac gcaacacccc tcgcgcaaca ctgcgccatc
     4021 tctgtaacat caccgacaac acggcaatcc cacaacgtct cggtgcaata tcctctgaaa
     4081 tggtttggtt ttcactccac ctcgtcggca tcctgaccag gataggaata tgtgtcgttc
     4141 cgcaccatgt acaaaacgtt gtcaactgac aacgttcttc aaatcaattg gataagacaa
     4201 atggccattt cccgccaatt catctgaatc aagacgttgt caactgacaa caaaatgtac
     4261 attgcaacag catgcacata tacatatttc cccggtgagc agtacccatt accatgtttg
     4321 gtaggtcccg atggtcgggg ccgggtcgcc ggttccggt
//
LOCUS       DAWBMW010000132         4282 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_4336_length_4282_cov_2.889520, whole genome shotgun sequence.
ACCESSION   DAWBMW010000132 DAWBMW010000000
VERSION     DAWBMW010000132.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4282)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4336_length_4282_cov_2.889520"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          806 a         1251 c         1314 g          911 t
ORIGIN      
        1 ggcaccgcca gctacgccgg ccaggaagcc aaatacgcca tcgaacactg ggtgcgtatc
       61 cccgtggaaa tcgaactcgc gcacgaattc cgttaccgtg acccgattct gaccccgcgt
      121 acgctcgtgg tggccatttc tcagtccggc gaaaccatgg atactctgat ggccctgcgc
      181 cacgcgcgcg aacaggggtc caaggtgctg gccatctgca acacgcaggg tgcctccatt
      241 ccgcgcgaat ccgacgccgt gctctacaca cacgccggcc cggaagttgc cgtggcatcc
      301 accaaggcgt ttgtggcgca gatcaccgcc gcctacctgc tgggcctgta tctggcgcag
      361 gtcaagggcg cgatgttccg cgatgagatt catcaggtgc ttgactccct gaaagacatg
      421 ccgcgcaaga ttcagtgggt gctcgacacc cagcccaaga ccatccaggc ggccgccgag
      481 cgcatggtga acgccaactc cttcttgttc ctcggccgtc acgtgggcta cccggtggcg
      541 cttgagggtg cactgaagct caaggagatc gcctacacgt tcaccgaggg cttcgcggcc
      601 ggcgagctca agcatggacc gatcgcgctg gtggacgagg gtgagcccgt ggtgttcatc
      661 gtgccgccgc agcgcggccg caatgtgttg cacgccaagg tgatctctgg tatcgaggag
      721 gtcaaggctc gtggcgcgta catcatcgcc gtggccgagc agggcgaccc ggatgtggaa
      781 cggtacgctg acgtggtgtt ctggcgcccc gcctgcccaa cgctgatgag cccgctggtt
      841 gacgtggtgc cgctgcagct gttcgccatg gacatggcaa agctcaaggg ctacgacgtg
      901 gacaagcccc gcaacctcgc caagtccgtg accgttgagt aagccacatc ggtatgtgat
      961 tgacgagcct catattccgt ttgagatgga cgtgctgtat gaggacgagc ggatcatcgt
     1021 ggtggacaag ccgcattttc tggccaccat gccgcgcggc atgtggtatc ggcagaccgc
     1081 gctgatccga ttgcgtgagc ggctggggga gccggacatc acgccagcgc accgattgga
     1141 ccgcatgacc gccggcgtat tggtgttcgt gcgggatccg gcctgccgcg gcgcgtatca
     1201 gatgctgttc cagaaccggc aggccgtcaa ggtctatgaa tgcctggcgc cctgccggcc
     1261 gatcagccgc ccgcgattcg gcacgatcat gcaggtcgag ccgccacgcc cgttcccatt
     1321 gctgcgccga tcgcacatca ccaaggaaca cggcacgatg gccgcattcg aggaaccggg
     1381 tttggccaac gccgaaaccc tcatcgagcg cggcgacctc tgtttccctc cctctgacgg
     1441 gaggtggcgc gaagcgccgg agggtggtcc ccatccgggg aatcacccca tctgccgcta
     1501 cacactccac ccgcgcaccg gcaaaaccca ccaattgcgc gtccacatga attcgctcgg
     1561 cctgcccatc gtgggcgacg acttctaccc gcgcatccag acgcgcccct acgacgattt
     1621 cagccaacca ttgcagttgg tagcacgcat gctgcgattc accgatccaa taacaggcga
     1681 gaagcgcgaa ttcgtttccc gtgtgccgct taagatctga tttcttatgc attgagagtg
     1741 ttttcgctca tggtgtcacc attcatagag ttcggcgggc tgcggggttt tgcggacatc
     1801 cagcgacaag ccaagctcag tcagggcgct gagaatggtg gagaattcgg gattggggac
     1861 tcgggcgtgt tcaaattcga tcagccagcg acgtgacacg cccagccgtt gggccatgtc
     1921 ggtctgacta atgccctggc gttgcctatg aatcactggc aggtggatca ctggcaggta
     1981 ccagtggatg ccagcacatg ccgctcgtca gctgatgtcg agcacggaac cgcgttcgat
     2041 cagttccggc gggattacgg ttttgacgcc gaccggtatc ttctcgcctt cgcattgacg
     2101 gcggaccatg gagaaggcgg tggcgccaat gtcgtcaaag cgcagcttca tggtcgtcag
     2161 gttcaagcgc gggaccatat cgaccagcga atcatcgacg cccacgatgc tgacatcctc
     2221 gggcacgcgc ttgcccgctg actgcaaccc gagcattgcg ccgtatgcca tttggtcgtt
     2281 ggcggcgtag atggccgtgc agtcggggtc gtgggccaac gccacgccgg cctggtagcc
     2341 tgaatccgcg caccaatcgc cgatgtacat gctcggtatg cgtgctccca tttgttccaa
     2401 cgcttctcgc caaccgcggg cgcggctttc ggcggcgcgc gaggtggacg gtccggcaat
     2461 gtggtagacg gtcttgtgcc ccttgctcat caggtaatcg acgatagcgg tcgagcagcc
     2521 atactggtcg gcgtcaacgg tggggcagtg gtcggcggcg gattcgcaga tgagcacgac
     2581 tggaaattct tgcggaggct tgtatccgac gaaatcggag atgcgttcct ccagcactac
     2641 gatgacacca tccaccggct gacgctgcat gcgatcgatg gcggcggaaa gcgtgcgttc
     2701 cgcaccgtgg cgcatggtct ggatggtgac tgaataatcg tcatcggccg cgttggccac
     2761 aatagaatca aggatgcggg cgttgccgaa cgtggcggtg ttgaacatca ccacgccgat
     2821 atcctggaag cggccgtgct ttagggctcg ggccgcataa ttcggacgat acccaaggcg
     2881 ttccattgcg gcttcgacac gctggcgtgt ttcgggtttc accgcatcgc tgccattcgc
     2941 cacgcgggac actgtctgtg gcgaaacgcc ggcggctttc gctatgtctt gcatggacac
     3001 ctgtgttggc ctcgacattt caccctcgct tgactcatta atgtcggtgt ttcgtttgca
     3061 acgcttatct tagggtcgct tacggtaacg ataacatcgt tgtcttcgtg accattgtag
     3121 cagagtgact gagaaaccct taattcaaca cggtactcaa cacgattgga caatgttaca
     3181 aatggtgtta tgatgataac gcaaacattt tggtcaacct tcgaaggaga ggggttggga
     3241 atggcttcag ccgatatcgc agctgcgccc gtgcattcgg gcaaggctcg tgtgaataag
     3301 cacaaggccg attggcgtgg atggaaattc atgtggcctt ttgcggtggt gttcgtgttc
     3361 gtgttcatcg tgccgatcgt gtacgccgtt tatatttcgt tcttcaagaa gcagatgatc
     3421 ggcggcacca agttcgtcgg cttcgagaac tacgcgcgcc tgttgggtga tggccagttc
     3481 tggagcagtg ttggtcgtgt ggccctgttc acgtgcgtgc aggtgccgat catgctgtgc
     3541 ctgtcggctt tgatggccct ggcgttggat tcgatgaagc tgcatggcac gaggttcttc
     3601 cgtatctcga cgttcctgcc ctacgcggtg ccggcggtgg tgtcgacgtt gatctggggt
     3661 ttcgtgtatg gcgcgaagta cggcctggtg ggttcgttga acgatttcct gggcacggac
     3721 atcgacgtgc tcgccccttc cgtgttgttg gccagcatcg ggaacatcgt gacgtgggag
     3781 ttcacgggct acaacatgct catcttctac agctcgctgt cgaccatccc gcattccctg
     3841 tatgaggcgg cgagcatcga cggcgcgtcg gagtggcaga tcgtgaagag catcaagctt
     3901 cccgaattgc gcggcagcct ggctatcacg gtgatcttca gcatcatcgg ctcgttccag
     3961 ttgttcaacg agccgagcat tttgcagaac atggttcctg gtaatgcgat cacgacgtat
     4021 tacacgccga acatgtacgc gtacaacctg agcttctccg gcaaccagtc gaattatgcg
     4081 gccgcgttgg cgatcgtgat ggccgtgatc accatggcca tcgcctacat cgtccagctc
     4141 aacagcctca aggagcagat gaagtgacga cgactactgc tgttcgtgtt gaggaatcga
     4201 tgatgatcgt gaaggagcgt atgtgatgag tgcagcaacg acttcggccg ccggtcgcct
     4261 gaccgacgcc gagctcaagg cc
//
LOCUS       DAWBMW010000133         4268 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_4352_length_4268_cov_2.693093, whole genome shotgun sequence.
ACCESSION   DAWBMW010000133 DAWBMW010000000
VERSION     DAWBMW010000133.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 4268)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4268)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4268
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4352_length_4268_cov_2.693093"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          818 a         1375 c         1271 g          804 t
ORIGIN      
        1 gctacggcaa gggcgacttc gtgccggatc tgaccgtgaa ctacaccatc atcgataagt
       61 ccaccggcaa ggaagtcgag ggcggcaccg ccacctccgg taccttcatg cagatgaacg
      121 cttccgatgg cccgcactac ggcgccaacg tcaagctcga caaggctggc acctaccagc
      181 tgaagctctc catcgaatcc ccggcctcca agggttggat gctgcacgtt gacccggaga
      241 ccggcgtcaa gggccacttc tggaccgagc cgatcgaggt caccttcgac aactgggatt
      301 acactcctcg ccagtggtga ttgactaagc agaaagcccc ctcgtcagag ggggctttac
      361 gccgctttag ggagataatt cttaggacgg tctaaaactc ccctcagtcc gcttcgcgga
      421 cagctcccct cagagagggg agcctaaggt gaacagagaa aggacgcgaa tgcttgaaca
      481 attcgtggca gtgatgccgg gcactttggc ccccgcattg ctggtgatga gcctgagcgt
      541 gatgctcacg gtcggcgagg ggcgagataa gccgataagc tcgcattggc gtctcatcgg
      601 cctgatcgtc ggtctgatcg ccgccatcgt attcgccggc ctgcgcgcct cggccgtgat
      661 caatcagcgc acgttcgtca actacccggt gttgtggtgc gcgatcattg ccgacatcct
      721 gaccatcgtc gtggtggtgt tcgcacgccg catcaccacc aattggcagc agcacaaggt
      781 gttcatgcac atcgctaacg ccgtggccgc catcgacatc gcgctcaccc tgttctacgc
      841 gctgccggac gtgattctgc agctcgccat ctgggtggaa ccaggggaga ccgcattcac
      901 ttcggccatg ctgctgcgcg ctcttggttt cctgcttggc gtggccatgt ccattattgt
      961 ggccgccatc ttccgcacga tgcgcaccac tgccgtgcgc tgggcgttca ccgtggccgt
     1021 cgcggccatg atggtgattc tgctgattca acacttcact ggtcttgccc agattctgca
     1081 ggcgcgaggc ttcccgatga accatacgat gttcgtggcg ctggcttggt ccatcaacca
     1141 gaactccacg atgattatgg cgcaggcgct ggtgttcctg attcccgccg tggcctccat
     1201 cgtggccggt ttccgtatga aaacccatga tgtgccggga gccaacgagg ccacgttgcg
     1261 ccggcataag gtattccgcc gccgcgccat cgccgctgcc gtgtggagcc ttgtcgcagc
     1321 catcggcgtc acagcaaccc tgaccgtggg cgttgccgcg acccatcaga ccatcaccct
     1381 ttccccgccg gaagcctact cgctcaagga cggcgtggcc accatcccct tcagccaggt
     1441 cgaggacggc cacctgcacc gcttcgaata caaggccaag gacggcaccg tcatgcgctt
     1501 catcatcatc cgaaagaacg gtggcgcgta cggcatcggc ttggacgcct gcgagaactg
     1561 cggcgacgct ggctactacg agaaggacgg caagatcatc tgcaagaagt gcgaggtggc
     1621 catcaacctg gcgaccatcg gcttcaaggg tggctgcaac ccgataccat tcccgtacaa
     1681 gaccggccac ggcaagatca ccattcaaac tacggatctg gacgcgctga gcgcccactt
     1741 ccggtgagtc aggagacaag aatcatgttc tttctgcgta tgattactcg ctcgttcacc
     1801 cgacagctgc gccggcgcct gcttatcgcg ctgaccgtat gcctgtcggc gaccgtgtcc
     1861 gtatcgatgc tgggcgtcgt gttcgacgtg ggcgacaagc tcaacgccga gctgtccacc
     1921 tacggttcga acatcaccgt gcagcccaag gcggatgcgg tcgtttccga cctgtacaac
     1981 accgagtcgg gcggcgcgac gtccaccagc gacccgaccg cttttctgaa ggagtcggac
     2041 gcggccaaga tcaagacgat cttctggtcg ttcaacatca ccagcttcgc gccgcagctc
     2101 aacatccacg ccacggtgaa cggtaccaac gtgccggtcg tcggcacgtg gttcaacaag
     2161 aacctcaaga tctccaccgg cgagactacc gtcgtgggcg tggaaggcat gcgctcgtgg
     2221 tggcagctgg acggcaagtg gcccaaagac gattccgacc agggcgtgat cggcaagacg
     2281 ctggcctccg aactcggcgt gaccaccggc gacaccatca ccctcaacaa aaccaccgcc
     2341 tcaggcaaga agaacgagca gaaaatcaag ctcaccggtg tctacgattc cggcgacgag
     2401 gacaacggct cgctctacat cgcctcctcc acggcccagg tactcgccga cctgcccgat
     2461 tcggtcgaca aaatcgaggt caaggccctg accacgccgg agaacgacct ggcccgtaag
     2521 gccgccgcga acccggccgc gctgagccag gaggaatggg agacctggta ctgcaccgcc
     2581 tacccgagct cgatcgccta tcagatcgaa gaggtgattc cgggcgcggt ggccaagcag
     2641 gtgcgtcagg tggccgcgtt gcagggcaat gtgctgcaaa agacgcaggc cgtgatgatt
     2701 ctgatgaccg tgctgagcct gatcgccgca gccgtggccg tggccaacct gatggtcgcc
     2761 tcgatcggcg aacggtcggg cgagctggcg ttgctcaagg cgctcggtgc caccgatgcc
     2821 gcggtatccc gcctgatgct ggccgaaacg gcggcaatct cgctgctggg cgcgattgtg
     2881 ggcgcgctgc tcggttccgg cgtggcccag ctgataggcc gggtggtgtt cggttccggc
     2941 attacgatgc ggccgatggt gttcgtgctg gtgttcgtgc tgctggctgt caccgtgctg
     3001 ctggcgtccg cctcatcgat tcgttcgatt ctgaatctca agcccgcgga ggtgctccat
     3061 ggtcgctgag tttatctacg taggtgtgga aggagggaac cgatgaccaa caccggtatg
     3121 ttcttcagaa tgctgttcag cgccgtgttc cgtcggcgtt cgcgcgcggt gatggctgtg
     3181 gtggcgtcgc tggtcggcgc agccacactg ttctgtctgg cgatgatctg cctcgccgtg
     3241 ccgcagcaga tgaacgagga gatgcgtgcg tacggcgcga acctgattgt cacgcccacc
     3301 gaatcgacca atgctgacgg caaggccggc atcgacaagg cgatggtgca gcacaccact
     3361 gaaatggtca aggccaaggg ttccgcgaaa tacgccacct accgctacga gaacgtgcgc
     3421 gtcaacgcct cgccgtacgt gatggccggc gtgaatgcgg cgcaggtcaa gaacctcaac
     3481 catcactggg tggtcgacgg ctcgtggccg accgacggca aggtgctggt cggacgcgac
     3541 atcgccgacg ccatcggcct gagaatcggc tccgccatca ccatcggcta ccgtgcatcc
     3601 gataacgcca gcaccaacgg cacttcgtcg aactcctcga tcgaccagca aaccaaagac
     3661 ggccgtgtct ccagtgacat catggacacc tccggtaccg aattccgcgt ggccggcatt
     3721 gtggacaccg gcggcagcga agactcgatc atctacgcca ccaacgtcga cgtcaacaag
     3781 ctcaccggca tcacgcgcgg cgtggacgtc atcgagtatt cggccggcgc cagcgacctc
     3841 gcaggtctgg tgtccagcat caacgacatg acctccatgc acgtcaaggc acagcaggtc
     3901 accaagatca ccgcctccga cacccgcatc atcaccatgc tgcagacgct gttctggatc
     3961 gtctcgctgg tggtgctggt gctgacgctc gtcggcgtcg gcaccacaat cagctcgatc
     4021 gtctcgcagc gacgcaacga gattggcctg cgcaaggccc tcggtgctag ctcgcgtgcc
     4081 atcggcaccg agttctacat cgaatcgtcg ctctacggcc tgattggcgg cctcatcggc
     4141 accgcaatcg gctacggact cgccagttgg ctgtgcgtgg cggtgttcga acgctccatc
     4201 gtattcaact ggtggctggc cctgatttcc gtgctgttca gcgcgctggt cgccatcgtc
     4261 gcctccat
//
LOCUS       DAWBMW010000134         4246 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_4373_length_4246_cov_1.811262, whole genome shotgun sequence.
ACCESSION   DAWBMW010000134 DAWBMW010000000
VERSION     DAWBMW010000134.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4246)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4373_length_4246_cov_1.811262"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    796..805
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2093..2102
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2614..2623
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3537..3546
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          906 a         1330 c         1191 g          779 t
ORIGIN      
        1 gccgagaacc ccgaaggcga gttcaatccg aaaccaaagc gcggcgacgc ctacaagcgt
       61 gcgatggacc gtcagaacgc ccgccgccgc aaccataaca agcaaaccaa atgaaaggag
      121 ccatactcat ggcgcttcct attgaaatgg tgcatggcac cggcctgacc accgttgagg
      181 aaaacaatga gtggcgtttc ggcgagcaga cgggcggcgt ggtctccgtg accatcgtcc
      241 ccgaactgtt caacgtcgat gacgagactc tgcgcaacaa gtacctgacc ggggtcagcc
      301 cgacagccac gaccatctac atccgttccg gtattccgct cgccaagatc acgagcggca
      361 ccaacaaggg cgcttacggc ccgtatgacc cgaaggctac cgatggccgt cagaccgcca
      421 tcgccggcct gttggagtcc gccgtcgccg tgaacgtcac ctattccggc tggcaggtcg
      481 atgacaccta tgtgggcctt cgctaccgtg gcgacattat caagagcaag ctgccggtcg
      541 ttcccgccga cgaggccaag tggggcggct gcttctacga tgtcgaggat gatgctgtca
      601 ccgcattgtc cggttcggct ggcgctgccg gttccgctgg tgtgggcgtg aagtccatca
      661 ccttgaccaa gaacacctct ggtgccatca ccggtggcac ttgggtcggc accgacaaca
      721 agtcgaacac catcaccatc gcctgacacc ccgtctaaac cgattctttg aaacccgccc
      781 ctcgtggcgg ggtttnnnnn nnnnntctga aaggaaatat ccaatggcat tggacaagga
      841 aatcttcccg ccgagcgaag ccaccgaggt tgcgcaggcg ggcttcgatt acgtgaacgg
      901 cattctcccg ttctccacca tgtttcccat ccagtccaat gacggcgaat ggaccgtctc
      961 ttggacgccg aatctgccga cgctctccac gaacgccatg cagcgtcgtg cgctggacgc
     1021 cgagatcggc cacacttcga tggtcgaaca gtccgccgaa cagcatacgg gccttctgcc
     1081 cctgtccggc atggaccaca tcaccgaacg tgatatggcc aagcacgcga acgacaagca
     1141 gttcatccac gacaaggccg aagccaagac cacgcatctg ggccagaccg ccgccgtgac
     1201 ccttgaactt gagtccatct ccgcgatgat ggatggcaag atcaccatca acgagaacgg
     1261 cgcgaacgtt gtctactcgt tcggccgtcc ggccaagcag cataatcaga ctccgaccac
     1321 tctctggtcc caggctactt ccgacccgat tgccgacgtt cagggttgga ttgaggtcat
     1381 gcgcaagaac aagggccgta caccgcacgc cgcgttcacc acgtcgaagg tcatcgacgc
     1441 attgcgcgtc aacgaacagt tccgtcagga agtgtccggc atggacttgg ctcattccaa
     1501 gccacgactg acccgcgacc aggtgctggg cgttctcgcc agccagcttc agctgaacga
     1561 cgtgcgtatg ctcgacctcg aatacgagaa ccttgaactg gacggcggct tcaagatgga
     1621 cgtggacacc accacgctca tccccgatgc cacgttcgtc atgcttccct cgttcaacga
     1681 cccgaccctt ggcttcaccg cttccggccc gaccgccgaa gcccaaaact ccgagtatga
     1741 gatcagcaag agcgtcaacg acggtcttgt cgccgccatg ctctcccatc aggctccggc
     1801 caactacgat atctgggtca acggctccgc gctgcccgta ttgcaggatg ccgtcagcac
     1861 gttcaaggcc aacgtcctgt aggagccgtc atggcaagcg ttgacggcat cgactggatg
     1921 aaacacatgc aggtcagtct gcttgaccag cccgggctag ccgacgccta tccgaacgaa
     1981 tgggcgaagt cacgttgccg tatcgccgcc gaaatggcgt tgaccgaatc cggcgacgcg
     2041 gaaccccgtc tcaattcggg cgacctgagc gaggacacgt tcgcctacgt ggnnnnnnnn
     2101 nntggtctgc tcgatggtga ttcgcgtcat gcgatggcac cggttcaaat ccgagtcgaa
     2161 cggcaactat tcgtatgagg agcatgaccc ccagcctaat ccgcccgcct atgatgccag
     2221 tcccaacctg tatgtgagca aacgcgaaaa gcagttgctt gacggttacg cggagggaca
     2281 cggccccgta ggcaccattg gtgtcgggtt gagccgaatc tacggattgt gaggccctat
     2341 ggccgatgaa acattggact tgggacacct ttacgacggt gttgatttgg atgaactcgg
     2401 cggcgggcac ctgtacgacg ataccgagtt ggagcctcgt atcacggatg acctgctgca
     2461 ccgcgacatg atcgtggtgc agccgatgaa accggtcgaa accgtctacg gttccggaac
     2521 ggtgccggat ggggacgcct cctactgtta ctgctcgttc gagcctcgaa tcaataagaa
     2581 cagcacgttt tccaagaact gggcgcagga cacnnnnnnn nnntcaacac gcagttctgg
     2641 ctcgataacg cctgttacga ggttgacggc ccgcctatgg agatgcgtca cgcctcggat
     2701 gccgcccacc attggaacat caccgcgagg tgcatcggcc atgcgaccga ggacaacggg
     2761 ttgaaaccgc ctgtcccgcc cgaggggagc cgcacatggg gtacgtgaac ttgaagcccg
     2821 cgaatgtgct gaaccgtgac atggcgatac tgttcggagc cgaagcgacc cgtcccgtgg
     2881 cggagaaggt cgaagcgaaa gccaaggcgc tggccgacat gaaggcgaag cattcgtccg
     2941 tcgccaaccg catcgacatc agcactcacg ctcacggcac gcacaccgcc gtcatcatga
     3001 gcgtcaaggg ccgtgacggt tccgagatcg cctctcactt ggagttcggc tacttcaacc
     3061 ggtggctgga acacaagtac ggcatcaaaa gcccgctggc ttggatgccg ggattgttca
     3121 tcatgtcgga ggcgaaatat gtctgacccc acgatattcg acctttccgt aagggaacag
     3181 ttggatgcgg tcgccatgac acgcgcctac ctggacgccg tcgaatggaa ggaccgtgat
     3241 ttcaggccgg tcatccaacc ggaggtcacg cccgccacgg attcgctcct gttgtcccat
     3301 gacgtgattc tctaccattg cggtgctcct gagcagcccg actggaatct gaaggcttgg
     3361 atatggcagt acacgctgtc tttgacggtg ttgggccgtg acccggaacg ggtggcccgc
     3421 atctgcggat ggctgcaccg ttgcatatcc gcatggccct accggcccgg caccgactat
     3481 gggaagatcg ggcggatagt ggacaatccc ggtttcgagt cccggtcttc cggcgannnn
     3541 nnnnnntcgc gtggacttcc acgaaacgca tacaggccgc gtccccacgc ggctgacctt
     3601 atctgaaaaa ccatcaatca cacaatcaga ccccgcacgc ctacacggct gcggggtttt
     3661 ccatatttga aaggaaaacg atatggctga cgaaatcggc atccacgacg acggcgtgtt
     3721 gaccgccgtc cgaggaacga tcttcatggc gaaggccgag accatcatta cctccgcact
     3781 gctcaagcag ttcaccgtcg aggcggcgac cgtgggcgtg ggcgacggca tgtggacgaa
     3841 cctcggccac atgtcgaacg acaacctgcc cgagttcgcg ttggacggcg gcgacgccac
     3901 cacgttgagc acttggctca aggtggcgtt ccgcacccag tacgcccaga ccaccggcac
     3961 tgtgacgttc aattcggtgc agggcgacaa gggcacgttc aagaccttct acaacgcggt
     4021 cgatatgacc ggcgccggcg tggccttctc cttggagaag acccccatca acaagtccct
     4081 gttcatcctg tggtccgaca cgaacacgac cggccgtgcc ggcctgctgc tgccgaactc
     4141 ggacatcgcg ttctccagtc tgcctgctct ttccacggat tcgttcgtgg agttctccgc
     4201 tcaggcgaac atcaagacat ccagcacgct tccgcatgac aagaac
//
LOCUS       DAWBMW010000135         4213 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_4416_length_4213_cov_1.848485, whole genome shotgun sequence.
ACCESSION   DAWBMW010000135 DAWBMW010000000
VERSION     DAWBMW010000135.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 4213)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4213)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4213
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4416_length_4213_cov_1.848485"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1239..1248
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3103..3112
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          817 a         1264 c         1243 g          869 t
ORIGIN      
        1 gatgccgtcg gcgcgcttaa gccatcgtgt cgcgtcaatc ggagccgggg ccgccagcgc
       61 ggccttcttc acgtcctcaa gcgatacggt gagctcacgg gcgcgctgat cctccagcgc
      121 tcccgccacc agttcgtcca aaaccgacat ggtcctccct ttgttggtgt tcggtcatgc
      181 gccactctag gtcacgtcct gtcccgattg ccgaacccgt cctacatttt gggctttcgc
      241 aggcgattcc ggcttaggca gaacattggc ataaactggc acgcatgtac agacgtttcg
      301 cgctcgactt ggaatgggac gccgtggctg gaccgcacaa tcgacggcgc atccttgctc
      361 gccggcgtgc ggaactcagg cactgcgtta tcccgccgct tgcaatttca cgaggtcgcc
      421 ccgctgcagg gcgacggcaa acgcctgatt cagccgttgg attcgaccga gcccatgcgc
      481 ctgttttgac ggaacatctt tgacgagaga tcgtcactga atcattgttt gctatatatg
      541 tatcacagtg tcataactat gaaatcatat tgtggaattg tggtatatga agttcgcatg
      601 acaacgatgt caccgttggt aggtggacca gtaggtgccg ttgctgccgg tggtggtgct
      661 gaactgccga tgtgattcag tccaccagcc cgcgcagcag cgcgatttcg tcgcggtgcg
      721 tggtcgacgt ctgctccttg gtttcgagaa tcatcgggag cttggccatg gtcggatcgt
      781 tgaccagtcg cgtgaagaac gggatgccga gctgcccttc gccgatgttg gcgtgacggt
      841 ccttatgcga gccgaggccg aactgcgaat cgttcacatg aatcgccttg acccttgcca
      901 agccgatcac ctcatttaac tggcgcatca cgccgtcgta gtcattctcc aaatcgtaac
      961 cggcatccaa cgcatggcag gtgtcgaacg tgacgccgac gttcgcctta ttctccacgc
     1021 catccatgat cgtggcgagc tcatcgaaat tgcggccaca ttccgtgcct ttgccggcca
     1081 tggtctccag aagcaccatg acgccatccg cacgctcgaa cacctgattc agtccctcgc
     1141 tgatcagccg gcagcctgtc tcggagcctt ggcccacatg cgcgccggga tgaatgttga
     1201 tatacacctc ctggccggcc tcgcggatgg cggtcagcnn nnnnnnnnct cgatgtcctc
     1261 ggccaacgcc tcgatcgcga atgcgcgctt ggcctcatcc ttgccggcca gattgtagac
     1321 gtacggcgcg tggacgacca acggcccata gccctcagcc ttcagacgcg caccgaacgc
     1381 cgccgcaccg gccgggtcga gcgccttgga acgtttgcca tacggagatc gcgggaagaa
     1441 cgcgaacgcg gtgccgccct cctcatggga gcgttccagc agcgcattcc atccgccggc
     1501 cgtcgacaga tgcgatccga tatacagttc catcattcca aactcctcgt gttccactcg
     1561 ctgttcgtgt taccgctatc atatggtagg cggcatcgag cgccgcccgc acaaccgaca
     1621 cgagacacaa ctgttcaccc gaccaaagga gacccgagca tggcgtcagc cggcaatccc
     1681 aaggacgaca ccggcctcga acggaaaatg gagtcgcgcc acctgacgat gatctccctg
     1741 ggcggcgtaa tcggtaccgg cctgttcgtc agctccggct atacgattca tcaggcgggg
     1801 ccgttgggcg cgatcctcgc ttacgcggtc ggctccctgc tcgtctactg tgtgatggtc
     1861 tcgctgggcg aactgtccgt ggccatgccg tatgccggca gcttccacat gtatgcgaag
     1921 cggttcatcg gccccggcac cgcgttcacc attgctatcc tgtattggca gaactgggcg
     1981 gtggcgctcg catcggagtt cacggcggcc gggctgctga tgcagcgatg gtttcctcat
     2041 tcgccgtcct gggtgtggtc ggccgtgttc attgtggtgg tgttcgcgca tggtatggtc
     2101 gctggccaag gagcggctga ttcccgcgcg attcgccaag acgaatttcc gtggtgtgcc
     2161 ggtgtgggcc gtgctgttca gcatggcggg ttcgctgctg gcgctgctgt cttccgtgct
     2221 cgccgcctcg accgtgtaat ttggtgttgg tcgcagtatc cggtttggcg acgctggtgg
     2281 tgtggttctc cgtgtgcgtg tgccatatcc gtttccggcg tgaatgggcg agggacgggc
     2341 acagcgccga tgagctcgac taccgtgcgc ccggtttccc ggtgctgccg tggctggcca
     2401 tcgtgatgtg catcggcgcg ctggtgctcg tggtgctcga tgagacgcaa cgttcgaccc
     2461 tgattgcatg attccgttcg tcgcctgctg ttatgcggtc tattacgccc tcgaacggca
     2521 gctcaaacgc gaaagaatgc ctaactcggt ttcgaggggc tgattcggcg ttcgccggag
     2581 gtctacttgc gtttcaaggg gctgatgccc aagtagacaa ggctctgaaa tggcgttatg
     2641 tcgttgccac aaagccgttt atacttgcgg tttggcgttt gagcagcccc tcgaaagcca
     2701 agtagatgtc cccaccggac gaaatcagcc ccttgaaagc caagagcccc actttttcgg
     2761 cgaattacgt cttatatcag gtcgtacggc atctgatgct tgaccatcca gaagatgccg
     2821 aggccgatga acaccagcag attgaatccg ccgctatgcc accacaccac ggcgatcagc
     2881 gtcgccatgc gcgccacata gaacgccgca cgccgccagc cttctcgcgg ccgggtttcc
     2941 actgtcatat gcgtgaagct gccgccgagc gtacagccgc cgcgcaacca tggcaccacg
     3001 ccctcgaaga ggatgagcgc cgccaggaag aggatcgagc cggtccagtc gaacggttcc
     3061 atcgactgcc atgagccgtt ggagcccgag ggcaggttcg atnnnnnnnn nngttcgatt
     3121 ggacgatcat gacgaacaga tgcgtcggca tcacggcgaa cccgatcagc gtcatgtcga
     3181 tgatgaacgt gatcagccgc ctcatgaagc cgggtgtggt ggtcggcgtc gtatcggcga
     3241 cccgcgccgg aatcaggcgc agcgagaaca tggccagcgc gaacccgatc agcgcgccgg
     3301 tcgtgttcca caggagatcg tccacgtcga acagacggta ggcgcacggg aacacgccca
     3361 tgaggccggt cagctgcatc gtctccagaa acagcgacgt ggcgaacgac agtactgcgg
     3421 taaccggcag cggccaacgc cagatacggc ccatgatgaa gcccaacggc aggaagaaca
     3481 cgatattaaa cgcgatttgc agtacggcgg tgagaccatc ggtacggatg tcaccgatga
     3541 actgcagtgg gttgatctgc ggtgtcagat ggtgcgcggc gcaataggcc gccgcatcgg
     3601 ccggcatcgg atacagtgtg aagcacagca ggccgagcag atacagcacc gtgccatacg
     3661 ccactatggc cgacgacagt cggatgcgat tgtcgcggtg atacagcagc gccagcaccg
     3721 gcaccgtcag cagcatggac acaaacggcc acaatgccag cgcgaacgca aacggttcgg
     3781 aaaagttctt aagaaatccc atcttcaatg ctccctgctt cggcccgctc cgttcggttt
     3841 cggaccatga ctctcgactt ctgattccaa ggtacggaac atgccggcat gcgggtatcg
     3901 gccatgagag cgaaatcatt gggatgaaag gcgattcgta tcattcgcat ggatgaggaa
     3961 gatgcaccag ttgtgactga caattgcggc ctgcgaaccg gcaggatgaa tgggactgac
     4021 ccaactggcc cgtctcatct tgtagggtgt ttcgtgctct tgtaggcaga tgattccaat
     4081 ggcgatgatc ggaataccgc cattgcaatg acctctcgcc aaggccagct acctacaaga
     4141 tgctgaatct gcctacaaga tgaattcgcc ggtgaaccat gccggcatca ccgcttcgag
     4201 gtaagggcgc atc
//
LOCUS       DAWBMW010000136         4191 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_4435_length_4191_cov_2.796180, whole genome shotgun sequence.
ACCESSION   DAWBMW010000136 DAWBMW010000000
VERSION     DAWBMW010000136.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 4191)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4191)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4191
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4435_length_4191_cov_2.796180"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          750 a         1258 c         1373 g          810 t
ORIGIN      
        1 ccactggttg atggaggcga cgacctcgat cttgccggaa gacgcggcgc cgctggaatc
       61 ggacttgccg ctggcggcgt tgctggaacc gcaggcggcc atcgagagca gggtcgccgc
      121 ggcggccgcg atggcgagcg cgcgcttggc gttggacatg atggacatga ttgggttttc
      181 ctctctaatt agccgaacgg ctgggcgatg ctgctgttgc tgcatcatcg cccagccacc
      241 aacacgacag aataacatgt attgagaatt attatcattt gcatttacat cggcgtgtca
      301 tgctggcgtt ccaccctgcc atcaccctgg cggcgttcct gctggcctgg gcgagcaccg
      361 tggtaccggc ctgggtagcg gccaaacgca agctgcgtga caagccggct tccctacttc
      421 gggcatttca tgttcatgac ttcgggtggc tataagcacg tgttcggcga gcagcaccag
      481 tcgaacgcct atatggtgcg gctcaagaac cacgagacct ccaatgtgga gtcgcgcagc
      541 gccaagctca tgaagctgga cggcgtgaaa ggcatcgtgc agaacacgac ctcaaagaaa
      601 cagcatgctc tgcgcgctga attcagtgga atagcatcgg aataacaaat gggatcggct
      661 ttgaagccga tcccatttgt tattcgctca ctgctttctg agccttctgc gctttctgct
      721 ccttttggca atcagagcac agtccgaaga cctccaaggt gtgggattca acggtgaagc
      781 cgtggccgtc ggccactttg cgcagccaag cctcgctggg cgggtctatt tccacggttt
      841 tgccgcaccg cctgcacacc aagtggtggt gatgtccgtc gtcgccgcat aggcggaaca
      901 gctgctggcc gtccaggcgg atcgtgtcgg cggcaccggc atcggccaag gcgttgagct
      961 ggcggtatac ggtggccaag ccgattttcg agccttcgtc ctccagcctg cggtgcaggt
     1021 cctgagcgga gatgaactcc tcgcagtcgg ccaaggcggc gcgaatcgcg tccttctgct
     1081 tggtctggcg ttcgatatgt tcaaccatcg gtaaggtcac gccttctttt tcaggtcgtc
     1141 ccagcagccg gtggcctcaa ggtcagcgac cgtcttggcc gggtcgccgg tgagcacggt
     1201 gatgtggccc atcttgcggt cgtggcgcac ttcggccttg ccgtagtcgt gcacattcca
     1261 ctccgggtgg gtggcgatca gcgcgcgcgt cggttccaca tgctggccaa gtacgttgac
     1321 catcacggcc ggcgagagca gcttgggctt gggcagaggc cagccggcga tgccgcggat
     1381 gtgggcgtcg aactggtcca tcgagcaggc ctcgatggtg tagtggccgg agttgtgcgg
     1441 gcgcggggcg agctcgttga cgatgacctg atcgtccttg gtgatgaaca gttcgatggc
     1501 cagcgtgcca gccagttcga agcccttggc caggcgaatc gccagttcat gcgcttcgcc
     1561 agccacatgt tcggacacct cggcgggggc gatggtcata tgcaggatgt tgttgcggtg
     1621 ctcgttacgc acaatcggga aggtgacgaa gtccttgccg ttgccggaga ccagaatcga
     1681 agcctcgaat gcgaagtcga cgaagccttc caggatgctc ggcgggaagc caccaccacg
     1741 gtcggagcgg gagcggatct tctccaagtc ggcgtcggaa cgcagcacga gctgaccgtg
     1801 gccgtcgtag ccgccggaca cggtcttcag cacggccgga taatgaatat cacccaatgc
     1861 ggcgtcaagc tcatcgaagt tgttgacagc gcgccacggt gcggtggcgg tgccgtggtc
     1921 gttgatgaac tgcttctcgc gcacgcggtg ctgggtcacg cgcagcaagt cggtgccctg
     1981 cggggtggcg gtgatgccgc gcacctcgtc gatggcgccg gcgtccacgt tctcgaactc
     2041 gtaggtgagc acgtcagact gcacggccag atcatggatg gcggttttgt catcgtactc
     2101 ggcggtgatc tggaagtcgg ccacctgacc ggtcgggcag ttcggcgttg ggtcaagcac
     2161 gccgacgcgg aagcccatgt agcgggcgga cagagccatc atgcggccga gctggccgcc
     2221 gccgatgatg ccgatggtcg agcccggcat cagcatggag acgcggccgc ccgtttcttc
     2281 agacaagctg ggcattggat tcggccacct tttccttcag ttcgttacgg taatcctcaa
     2341 gcttgtcggc cagttcgggc tcgaaggcgg agagcatctg cacggcgagc aggccggcgt
     2401 tggtggcacc ggagttgccg acggcggtgg tggccaccgg aatgccgccg ggcatctgca
     2461 cgatggacag cagcgaatcc cagccggaca gggcgtggga gcgcacgggc acgccgatca
     2521 cgggcagtgt ggtctgcgcg gcgatcatgc ccggcaggtg ggcggcaccg ccggcaccgg
     2581 cgataatgac cttcaggcca ttggcgcggg cgttgtgcgc gaactcggcc atgagctcgg
     2641 gcgtgcggtg cgcggagatg acctgcttgt ggtaggcgac gccgaactgg tcgagaatat
     2701 cgcaggcgcg cttcatggtc tcccagtcgc tggccgatcc catgaccact gcgacttcgg
     2761 gcttggattc atttgccatt atcatttact ccctagttat tgagaataag gcccactgta
     2821 ctcctgatag cggagttaag ccacgcagcg aatcgttctc aatacataat tctgtgaggc
     2881 ttacagctca ataacgatct tgccgaacat gtcgccggcg atcattctgg cgaacgcctc
     2941 gggggcggcg tccaacccgc tgaccacgga atcgatcacc ggatgcaggt cggcgtgctc
     3001 cacgaagcgc agcaatcgtg cgaaatcctc gcgcgaaccc atggtgcagc cctgcacgcg
     3061 aatgtcctgg aagaacagtc gcgtgagctc cgcaccaggc tggtcgccgg tggtggcgcc
     3121 gcagatggcg atggtgccgc cggggcgcac ggatttcatc gaatggctcc aagtagccgc
     3181 gcccacggat tcgatcaccg catcgacctt gcgcggcagg cgggcaccga tctccagcgc
     3241 ggcgttcgcg ccaagtttca gcgccttgga gcgcttgtcc gcgtcgcgcg aggtgacgaa
     3301 cacctccaga ccggcggcat gggcgagctg gatggccgca gtggacacgc cgccgcccgc
     3361 gccctgaatg agcacggagt cgcccggctt gatgccggcg gcatcgaaca acagcgagta
     3421 ggcggtcagc cagctggtgc cgagtgcggc cgcctcagcc gtggtgaggc ttgcgggctt
     3481 ggcgaacacg ttggcggacg gcacggaagt cttctcggcc atcgtgccgg ggtatttctc
     3541 ggagaggatc gtgcggcgct cgcccggggc cacgcccgcg ccatcggtgc cgacgaacgt
     3601 gtacagcacc acttcgctgc cggccttgag cccatgcttg ccggggatgt cctcgtccag
     3661 aatgcccacg gcatcggtgc ccaggatcat cggcgtctgt tcggcggcga gacccacacc
     3721 ctgtagcgac cagacgtcgt gatggtttac ggtcaccgcg cgcaccgaaa cggtcgacca
     3781 gaacgggcgg gcctggggtt cgggctgctc gcccactgcc aacgcggcaa gcggatcggc
     3841 atggttgacg gtggatgcgt atacggcctt cattgcgcgc tccttggtat caaaaggaat
     3901 taccgcattc attgtcccac atcgcgcagc gaacgaatca cccatttacg gagatgttgg
     3961 gggcattgct tcgtcatgtg gacctcggac aatgccgggc gttagcattc aacttcggaa
     4021 agactagcga agggagattc gagggtatgc gcagcgcgtc aggaaacgga tttgattcaa
     4081 gcgattcctt cgccgtctcc ggcgctgccg agaggaccgc acgccttcac gacatacccc
     4141 cgagcattac cccttcccat ggcgacgact cccatctgct gagccgcatt g
//
LOCUS       DAWBMW010000137         4149 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_4486_length_4149_cov_2.483146, whole genome shotgun sequence.
ACCESSION   DAWBMW010000137 DAWBMW010000000
VERSION     DAWBMW010000137.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4149)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4486_length_4149_cov_2.483146"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3129..3138
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          936 a         1246 c         1189 g          768 t
ORIGIN      
        1 cgcccacgag atcatggacg aggaaggccg tcgcatcccg atcattgcca agatcgagaa
       61 gccgcaggct ctggagaacc tcgaagagat cgtcaagacc ttcgacggcg tcatggccgc
      121 ccgtggcgac atggccgttg agtgcccgct ggaagaggtc ccgctggcca ctaagcgcat
      181 catcgagctg gctcgccagt acgccaagcc ggtcatcgtg gctaccgaag tcctcggctc
      241 catggtgaac tccccggttc cgactcgtgc agaggcttcc gactgcgcca acgccatcct
      301 cgacggctcc gacgccacca tgacctccaa cgagaccgcc gttggtaagt acccggatgt
      361 gaccgtggcc accatggccc gtatctccgg ctacgccacc gaccacggct tcgaccgtat
      421 cccggagctc aagaaccttg acatgtcctc caccggcgct gtctcctccg ccgctgtgga
      481 cctggccgac aagctcaacg ccaaggccat cgttgcttac acccagaccg gtgccaccgt
      541 gcaccgcgtc tcccgtgagc gtccggccgc cccgatctac ggcctgacca gcaacgagca
      601 cacctaccac tggctggctc tgtcctgggg caccgaagcc ttcctgctca aggaggacta
      661 ccacgacaag tcccgcaagg atctcatggt ctacacggac aaggtcctca aggatgctgg
      721 caaggttgct gacggcgaca agatcgtcat cctgagctct gctcagggcg atcaccagcc
      781 gggccgcact gactccatct acgtgcacac cgttggcgct tgcgactgat ttcaggcttc
      841 tggtagctgc tgacagctag catatagcac acgaaagccc ccatccaaag tggatggggg
      901 ctttcgttat ctgttatgtt gcgattgtgt ggtgattgca atggcccaca gtcaccgcgg
      961 tgatcgagcg tggcgatcag tcgttgagat gatcgagcgt gccgctcttc aggcgcaaaa
     1021 tggcgaccat ggtcttggca tccacaatcg tgccattgac caccatctca taggcatcgt
     1081 ccaccgacac catacgcacg tcggaacgcg gagtctccgg gccgatttcc tgacgggaag
     1141 ccggggtaag accggtggcg aagaagattg cggtgtgctg ggtggaatag ctcggcgtgt
     1201 tcataaaacg ggtgaactgc gtaagcttgg tggcgtcgaa accggcctct tcggccagct
     1261 tgcgcttagc tacatcggtc gggcgttcgg acgggttgtt ggcgtgaccg gccggaatct
     1321 ccaacgtcca acgatggttg gcgatgcgat actgctcgac cagcggaatc atgccatcat
     1381 cggtgacggc cagcacagcc acagtgtcgc cattgttctc ctgaacaaag tagcgttcaa
     1441 acgaaccgat ttgattcgac tcataggaga cgtgatcgac attgaaataa tgcgtctcca
     1501 caatctgtct acgggaaatc tccttgaccg gagaggggcg ccagggatcg aactgacggt
     1561 aagtttcgtt gctcataagc cctcctaaca aaagcgatac atcgaaacta cagcccattg
     1621 taagcggtga aaccacgcag gagaacgggc aacacacggg tgcgccgtcc gcgatatcga
     1681 cgtgtttcgc ctgtttcacc ggggggaatg gaggattggc gcgtgtcttg tgtcgcaaga
     1741 gtcgaattgg tatgctactc tcttcagagt gctttggcac gccctcgtat cccaattggt
     1801 agaggaagca gcctcaaaat ctgcgcagtg tgggttcgag tcccaccgag ggcactttag
     1861 ttaggagttc gctgaaatta ttgtgaaccc cattgttggg taattcaaga aagaggttgg
     1921 catggctgca aggaagaagt ccgaagaacc gagtatcaac atcgatgagg tgctgaccga
     1981 cgaagagctc aaggccgtgg ccaatgaaag cgaacctgct gagcgcaagg ccaagcccga
     2041 ggacggtccg cgtaccgttg tcgtggccga ggacgaagcc gtgaaccgca tggatctggt
     2101 cgccatgctg gaagacaacg gttatgaggt cgttggtcag gccgccaacg gcgaggaagc
     2161 cgtggaactg acccgcaagt accgccctga tgtggtgtgc atggatgtca agatgccgcg
     2221 tatggacggc atcaccgctg ccggcattat ctgcgatgaa aacatcgccc ccgtcgtcat
     2281 gctcaccgca ttctcccaga ccgatctggt gaagaaggcc accggcgctg gcgccatggc
     2341 atacgtcacc aagccgtacg aggaatccaa gctgctgccg accctcgagg tggccatggg
     2401 ccgtttcgcc gagatcaacg atttgctgga cagcgtggag cgcagcgaac gtaagctcaa
     2461 ggaaaccacc gatcagctca aggaaaccga agagaagctc aagaaggccg aagacacgct
     2521 tgaggagcgc aagctcgtcg accgcgccaa gggtctgctg atggacaagg cggacttctc
     2581 cgagcagggt gccttccgtt ggattcagaa gacctccatg gaccagcgca tccccaagaa
     2641 gcgcctggct caggccatca tcgccaagta cggcgatccg aagccttctg attcgggcga
     2701 acgctgatta tcggcgtcgc cgtactgagt cgtaaagcaa cacaaacaca ctatgggaga
     2761 ggccagcgtg accgtaccca ccaatgaaac attgctggtg gtggacggcc actcgctggc
     2821 cttccgtgca tttttcgccc tgccggtcga caacttcagc acctccagcg gccaggccac
     2881 caacgccgtc tggggttttg ccaccatgct cgcgcaggtg atcgatgcgg agaagcccga
     2941 ccatctgggc gtcgccttcg atgtgaaggg cggcacattc cgcaatgaga tgcttccgca
     3001 atacaaaggc acccgtgagg cggctcccga agagctgctc acccagctgc cattgattca
     3061 acgcatgctc acggccctcg gcgtcaccta catcgagaag ccgggattcg aaggcgacga
     3121 cgtgatcgnn nnnnnnnnct tcaaagacct gaagcatatg acgccgcagt ccatcatcga
     3181 caaatacaaa gtcactcccg cacaatatcc ggatctggcg gcactccgag gcgaaaccgc
     3241 cgacaatatt cccggtgtgc ccggcgtagg cgacggcttt gcggccaagt ggatcaacca
     3301 attcggttcc ctcgacggca tttgcgagca cgccgatgaa atcggcggca aaaaaggcga
     3361 atcactgcgg gccaatatcg accaggtcaa actcaatcgc aaagtcaacg cactggtacg
     3421 agacgtcgat ctaggcgtcg atatcgagga tctgacattc ggcaccgtgg acgtggcaca
     3481 gatcgacgcc ctgttcaagg agcttgaatt cggacctcgc accaagagtc gtgtactgaa
     3541 aaccttcaat accggggcca aggcatccaa cacatccgga gctggcgagt ctacgaataa
     3601 tgagcagaac gagcaggata gcagcctcga tctgaacctg ccggaaccca cgagcatcac
     3661 tgctccggag cagttcgacg aatgggtcaa ggcccatcgc gtcgaagtca aggttcccgg
     3721 tgaaatcgcc gatttcacgg tttccgacta tggcgatggc tcgcagcggc atgccatctg
     3781 cggcgacgcg gtcggccacg cttggaccgt ggccgcatgg ggcgacgaaa ggccggggcg
     3841 tgccacggca caagccattg ccgtggccac ggcaacatca gcggccatag tgccccttcc
     3901 aatcaccgat accctgcgag cacagcttgc gcggttcctg aaaagcgaac actcccgcac
     3961 catcgtgcat gggtataaag aattgctgca tcttttgggc gcggtcgatc ttgacatgga
     4021 cctgccgatg ttcgacacca agcttgccgg gtatctggcc caaccggatt tccatgccga
     4081 tagcctcaag caggcggccg agcacttcct cgacatccac ttcaccgaaa ccgaacagcc
     4141 ttcgcaagg
//
LOCUS       DAWBMW010000138         4134 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_4510_length_4134_cov_2.060554, whole genome shotgun sequence.
ACCESSION   DAWBMW010000138 DAWBMW010000000
VERSION     DAWBMW010000138.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 4134)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4134)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4134
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4510_length_4134_cov_2.060554"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    365..374
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1215..1224
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2868..2877
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          908 a         1284 c         1226 g          686 t
ORIGIN      
        1 gcagtagtag tcggcgacgg caagccgttc gtgtccgcac ttatcgagct cgacgccgaa
       61 atgctgcatt cctggctgga aggtcagggc ctcaatgccg acatgacact cgccgaggcc
      121 agcgacaacg acgcggtgcg cgcctttatc cagcagtaca tcgaccaggc caacgccaat
      181 gtctcccgcg ctgagtccgt gcgcaaattc gcggtgctgg atgaggaatt cagtcaggag
      241 cacggcacac tcaccccgag catgaaagtg gtgcgtccca aggtattgca gcgctacgcc
      301 accgtcatcg aggaggatct gtacgcgccc aagccatcca acaagccgct gcccgccgct
      361 gccannnnnn nnnncgctgc caagatcatc gacagcacgc tggagaccgt caagaaatcc
      421 tccgaatcgg tcaagcaggc gtccgaacag gtcaaacagg cctcggaaca gatgaaaacc
      481 agcgtgtctg attcgattgc tagcgtgtcg gagaagatca agaagtcgaa ggccgaaccc
      541 gaagagggcg agaccggcga ttccgctgac aatgccgctg acaccggcag caagccggac
      601 cagccggctg acgagaagaa tgaggagtaa cgagaagtgg ccatccgtga gatcagggtc
      661 gtgcccgacc cagtcctgcg caccccctgc gatgagatca aggaaatcac cccggccgtg
      721 cgccgcctgg ttgacgacct gttggaaacc gtggacgatc cgggccgagc gggcctgtcc
      781 gccaaccaga tcggcgtgag cctgagggcc ttctcctaca acatcgacgg caaggtcggc
      841 tacgttctga accccgtact cgaggagaag tccggcgagc agtatggcga tgaaggttgt
      901 ctgtccgtgc ccggcttgtg gtacaagacc cgtcgtgccg actacgctcg cgtgcgtggc
      961 atcgacttgg acggcaacga ggtcgtgctc gagggctccg ggctgatggg ccgtatgctt
     1021 cagcacgaat gcgaccacct cgatggccac gtgtatttgg atcgcctgga aaaggaagaa
     1081 cgccgcgaag ccctgcgcta catgcgcaat cgtcaggcga agtagtccat actcggctcc
     1141 cactggctgg agccgtcgaa cgtagtgaga ctgagggtgg ttctgccggt accgcccgtg
     1201 actcccctca gtcgnnnnnn nnnnaacgga cgcagtcgct ggtgtccaga gccacccaat
     1261 attgtcaacc ggcacacaac aaattcgcgt catgctcgcg acggcttgtg tcggtcggct
     1321 gcacaactcg tgaaaccggg ggagcagctg cacgcaggtg cgcatggcca ggcaagaacc
     1381 gacaatgaaa ggtagtaata ccatggctca gatcactatg agcgatatgc tgaaggctgg
     1441 cctgcacttc ggccaccaga cccgtcgttg gaacccgaag atgaagcagt tcatcctcac
     1501 ccagcgcaac ggcatccacc gtggcccaca acggcaccgt gctcttcgtc ggcaccaaga
     1561 agcaggctca ggaagctatc gccaaccagg ccacccgcgt gaacatgccc tacgtctccg
     1621 agcgttggct cggcggcatg ctgaccaact tccagaccgt ctccaagcgc gtcaaccgcc
     1681 tcaaggaact tgaggaaatg gacttcaccg acgtgcacgg ttccggcctg accaagaagg
     1741 agctcctcct gctcgagcgc gagaaggaca agctcaacaa gcagctgggc ggtatccgca
     1801 acatgaaccg caccccgtcc gcgatgttcg tcgtcgacat caccaaggaa gccctggctg
     1861 ttgaggaagc tcacaagctg ggtatcccgg ttgtcgccat cgtcgacacc aacgccgacc
     1921 cggacaccgt tgagtacccg atcccggcca acgatgacgc catccgcggc atcgaactgc
     1981 tgaccagcct gatggctgac gccgtcgccg aaggtctgct ggagcgctcc ggcgccaaca
     2041 aggccgaggg cgaagccgcc gagcagccga tggccgcttg ggagaaggaa ctcctgacca
     2101 acgaggctcc tgccgaggct tccgctgaag ccgccgctcc ggctgccgcc gagggcgaaa
     2161 ccgccgaggc ccccaaggcc gagtgatgct ctacgatggg aagtgcctga atgagcgctt
     2221 cccatcgttc atgggtaagc aaaatttctc aaggagaata atcaatggca gcaattaccg
     2281 ccgcactgat caagcaggtt cgtgaagaca ccggcgccgg catgctcgac gtcaagaagg
     2341 ctctgaccga agccgaaggc gacgtcgccc gcgccaagga aatcatccgc gccaagggta
     2401 tcgccgcagc cggcaagcgt gaaggccgca aggctcagga aggcaccatc gcctccaagg
     2461 tcgtcgagac cgccaatggc gagaccggct acgccgtcga gctgaactcc gagaccgact
     2521 tcgtggccaa gaccccgaag ttcgtcgagt tcaccgagga agttctcggc tacgccgtgg
     2581 acgccgacgc caactccgct gacgagctgc ttgaggccaa ggctggcgac accaccgtca
     2641 agctggctgt cgaagaggcc gccgccctgt tcggtgagca cgtcaaggtc ggccagttcg
     2701 ccaagatctc cggcgagcac gtcgaagtct acgctcacaa gaagtccgcc gagatgccgc
     2761 cgagcatcgt cgccatgatc gccaccgaca aggccggcgc cgccgtggct cacgaagctg
     2821 ctctgcagat ctccgccatg ggcgccaagt ggctgacccg cgaggacnnn nnnnnnnatg
     2881 tggtcgagtc cgagcgtcgc gtggccaccg agaagtccct ggccgagggc aagccggaga
     2941 agatcgttcc gaagatcgtc gaaggccgtc tcaacgcttt cttcaaggaa gtcgtgctgc
     3001 ttgagcagcc gttcgtcaag gacccgtcca agaccgtcgg cgacctgttc aaggaagtcg
     3061 gtggcaacgc caccgcattc gctcgtgtcg aggtcggcaa gggcgaagag gagtgagcct
     3121 aaggcttact ccacgcgctt agcgtaattt caacgaaacg ccatccggtt attcaataat
     3181 cggatggcgt ttcgctattc caagggtgtc gttgctaggg ggtgtcgtcg taaaccgata
     3241 cgcttgtcta cggataattt cgcactttcg gttttgagaa aggcacaata tgactgatag
     3301 cgacaatccg cgcagagttc tgctcaagct gtccggcgaa gcattcggtg gtggcaaggt
     3361 cggcatcgat acccaggtga ttcgtcgcat tgccgaggag atcgttccgg ccgtacaaca
     3421 gggtgtgcag gtggccatcg tggtcggcgg cggcaacttc ttccgcggtg ccgaactgca
     3481 gcaggccggt atcgatcgtt cccgtggcga ctacatgggt atgctcggca cggtgatgaa
     3541 ctgcctcgct ctacaggact ttctggagca ggaggggcag gccacccgcg tgcagaccgc
     3601 catcaccatg ggacaggtcg ccgagccgta tattccgctc aaggccatcc gtcaccttga
     3661 aaagggtcgc gtggtgattt tcggcgccgg tgccggcatg ccgtacttct ccaccgacac
     3721 ggtttccatc cagcgttccc tcgaaatcca ctgcgacgag gtacttatgg gtaagaacgg
     3781 cgtcgacggc gtctacaccg ccgactcgcg caaggacgag aacgccaagc gttttgaaac
     3841 cctgagctac aatcgcgccc tcgtggacaa tctggccgtc atggacgcct ccgcactgtc
     3901 catggcccgc gacaacaaga agcgcatccg cgtcttcggc ctcgaagagc cgggcaacgt
     3961 gacccgcgcc ctcgtcggcg atgaaatcgg cacgctggtc tccaccgccg aatcgcgcgt
     4021 ggccgagtaa gccgcaaacg cccacaatcg aatcaagcgt tcatagacga acaacactga
     4081 cgaacaccaa cacacaagga gtgaatatgt ctcttatcga ccaggccaag gaac
//
LOCUS       DAWBMW010000139         4128 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_4522_length_4128_cov_2.870611, whole genome shotgun sequence.
ACCESSION   DAWBMW010000139 DAWBMW010000000
VERSION     DAWBMW010000139.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4128)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4522_length_4128_cov_2.870611"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          801 a         1269 c         1216 g          842 t
ORIGIN      
        1 ggccactgca tccaaggcac tgcacaacaa accccgtatc agcgaagaaa ccaagcagcg
       61 tgtactggat gccgccaagc aactcaacta ctctcccaac aaactcgccc aatccctagc
      121 ccgcggcacc accggcacta tcggcctggt cacctccgat ctgcagggtc gtttctccac
      181 accgattctt atcggcgcgg agaacgagtt gcgagcccaa tcgacctcag tattgttggc
      241 caacgctcgt ggcgacgccg ccttggaacg tcagcatgtc gagaaactgc tgtccctcaa
      301 ggtcgacgga ctgctcatcg tgcagtgcga gaccaacccc aggcccacgc ttggccatga
      361 ctggggcgtg ccactggtct acgtctacgg cccctccacc gaccccaaag actgctcggt
      421 cacttgcgac aacgtggacg ccggccgcat ggccatcaac catctcatct cgtgtggccg
      481 ccggcacatc gccattatcg gcggcgacga aacctatacc gctgccaccg accgcaccaa
      541 gggcgcgctc gaggcgctct ccgaattagg tattgaaccc gccggcccaa ttcgttacgg
      601 caaatgggac gaaggctggg gccgtgccgc cacccgtctg ctgctcgacc aaggggtcaa
      661 gttcgatgca gtggtctgcc agaacgatca gttggcccgc ggctgcattg acgtgctcaa
      721 gcagcaggga ctgcgcatcc cggatgacgt cgccgtcatc ggccacgata actggtcggt
      781 actgacctcc agctcgaggc ccgcactgac gtcgatcgac aacgaaaccg aaaccatcgg
      841 acggcgtgcg gcacgatact tggtcgatgc catcgacggc aacccgcatc acggtgtcga
      901 ctacgtaccc tgccacctta tccagcgcga atccaccctc ccgctggact gatatcaatc
      961 gatgccctac cgcttcttgg ttttgcgccc ctttaagtag atgtcgatga cgatccatac
     1021 gccgagggcg aaggccacga cgtagcccat gaagccgatg accggaatgc cgaagataat
     1081 cggcttcatg ccggcgtagt agacaatcga cgagccaacg aacaaaccga ccacaatcag
     1141 ggccatcgtc agacgattca ccatatccga aagcagctgg aacggctctt cggagccgac
     1201 cagttccatg ttgacgcgca actgaccacg ggtcagcata cgggccacgg tcttgagttc
     1261 ggtcatggtg ccgagcgtgg catgcaaagc ctgatgtccc tcaacgccca gcgacttgat
     1321 ctcgtctttg gtggcgttct tcagcgattt cgacgtggcg atatggtcgg aaatgatttc
     1381 gatcatattc acatcaggaa tgaactcgtc cagcaagcct tcgagcgtaa ccaacgcgcg
     1441 gccgacggtg gtcaccgtac tgggcacctc gatgttgtgg cggccggcca tctgcgtcag
     1501 tgcggtcagg aaggcggcaa tgtccagttc ggccagatcc acggtgccga attccttgac
     1561 gatcacgtcc agatcagcca gcagtgctgg gtagtcctcc ggatcggcct cggtgccggc
     1621 aaatcgcagc agcccctcgg ccagagcggg cgaatcctgt ttggcgacgg cgaaaatcat
     1681 ttctttgagg actgcacggg tgcgctggtc gagccgaccg gtcataccga ggtcgataag
     1741 tacgatctgg ccgccccgga tgatgatatt gccgggatgt gggtcagcgt ggaagaagcc
     1801 gtcgtcgaga atctgcgtgg cgtaattgtc gacgagcttg gtgccgatct ccttcaagtc
     1861 ataaccagca tcgacgagcc ggcccgggtg ggagacggag atgccgtcga tgtactccat
     1921 gaccaccaca tgctcggtgc acagttcggt gtacgccgtc gggcagtcca tgtatttgaa
     1981 tcgggcggcg aaacgcttga attcggcgag gttgcgggcc tcgatcagaa agtcagtctc
     2041 cgattcgaag gtgtcccaca gctcctcgac cacgcccttc aggtccacga tctgtgaggt
     2101 gcgaatcacc ttagtggcgg ccttggctat ggagcgcatg atggagacgt cctgagccat
     2161 ggtttcgcgc acacccgggc gctgcacttt aatggccaca tcttcgccgg ttttcagcgt
     2221 ggcgcgatgc acttgcgcca gtgaagcaga gcccaacggc ttgggatcga tatgcgcgaa
     2281 cacctcgtcg gcgggacgcc cgtattcggc ggcaagcaca tccaacaccg tggaatacgg
     2341 catcgggtcg gcgtcggcgc gcagtttggc gagttcgtcg cagaagcttt gcggcagaat
     2401 ctcggagcgc atcgacaaaa tctggcccac tttgacgaac gtggggccga gcgcctcgaa
     2461 catgaggcgc attttgaccg gagtcaggcc gtgcagcacg tcgaattgct cgacgattct
     2521 ggtgatctgc cccagtcgtt tgatcttgcc acgacgcgtc agatggtagc gcgtggcgaa
     2581 atcgttcctg cgtccgaaca gtgccagcgt ttcgtcggat acgctcggag tcgccttggt
     2641 aacggtctgc ttgagcgtgt ccgacgtgtc gacggaccgg ttcgccgccg cctcacgttc
     2701 ggctttcgca ggcgtcagaa tagggtcgac cctgctggaa agcggggtcg accctgattc
     2761 cggcgtagcg cgtgtgggct tggtcactcg gccttgccct cgtcagcggg cttgtcggcg
     2821 gcactggtgt cggctgcggc agcagctttg tcgccggctt cggcggcggc cttcttggcg
     2881 tcatccaccg tctcgctcac cttgcgcttg agctcggtgt tcagcgcctt gccctgctcg
     2941 accgtaatct cacccttctt gacgagttcg tcgacgagct cgctgctctt ctcgtatccg
     3001 gtggcaaggg ctccgatgcc cgccagcagg accttgcgca ggccatcacc cagaagatca
     3061 aaatcagcca tgatacccct ccttcgaggt ccgacatggc ctggtgccat gtctcgtacc
     3121 accaagccta gcgcaaacaa cagggcaatg tactagaccc tcagactgat ttcgactgat
     3181 ttttcgctga ttggtactta ccgaggactc accgagaatt caccgaggac gttcggcggc
     3241 agccttgcgc gcgttggtgg ctacttgatc gagccgggcg agctccttgg cttgttcggg
     3301 ggtctgatcg gacgtatcga acgtaccgta acgacgagtc acggcagttt cgataaggaa
     3361 atcgctgatg gcggggtttt taggcagcaa cgagccgtgc atgtaggtgc cgatcacgtt
     3421 gtgcacgcgc gcgccctcgg tatggtcttc gccattgttg cctcggccgt cctgatcgac
     3481 ggtgccgagt ggctgaacgc cttcgcgcag gaaggtctgg ccggagtggt tctcgtagcc
     3541 aatcacatcg ccgaactggt cggaatgctc aacaagattg ccgatcatac gcacgttctg
     3601 gcccacggta taagcaccga taacgccgat gccgtccaga cgggtgccgt ccacggtttc
     3661 aaagtactcg ccgaacagct ggtacaggcc gcagatcatc agcatcggcg taccgtctgc
     3721 ggcaagcgaa cgcaacaggt cggcgcgatg gtagaagtcg tcgatgattt tcttctggcc
     3781 ggtgtcttgg ccgccgcctc ccagaataag gtccacctgg tcgggccagt cgtcgccttg
     3841 attgtactgg tggacgaccg gttcataacc gtacagttcc agacggcggc ggatagtcag
     3901 cacgttgccg gaatcgccgt agatgttcat gtctttggga tacagggaca ccacatcaat
     3961 tgttctggcc atgtcacgtt ctccttgtcg atgtcttccc gctgacggga agcgtcttat
     4021 tcctcaccat gtcctcccgc aggcgggagg tggcgcgcgg gcgctagagg atagttactt
     4081 gcccacgccc gcgtcccgta cttcggtgat gcggcccagt acggcgcg
//
LOCUS       DAWBMW010000140         4119 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_4534_length_4119_cov_3.423474, whole genome shotgun sequence.
ACCESSION   DAWBMW010000140 DAWBMW010000000
VERSION     DAWBMW010000140.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 4119)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4119)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4119
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4534_length_4119_cov_3.423474"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1460..1469
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          815 a         1337 c         1264 g          693 t
ORIGIN      
        1 cgacttcgtg tacaccgacg ccgaacgcga ccgcgtgctc gccgaaggca aggccaacgg
       61 acgccagctg cccaagggcg aaggcatcca gcgctacaag ggtctgggcg agatgagcta
      121 ccaggagcta tgggagacca ccatggaccc cgagcaccgc attctgaagc aggtgcacat
      181 cgaggacgcc gccgcggccg acgagacctt ctcgatgctg atgggcgatg aggtcgaacc
      241 ccgccgcctc ttcatccagc gcaacgccaa gaacgtgcgt tggatcgatg cgtaacggaa
      301 tcctccaacc tccctcgtca gagggaggta cacaacggac ttagacaact aaaggaacaa
      361 cagtggcaga tgaaaacaac accggcgacg agcagttcac gccggacggc tcgatggagc
      421 cgttgagccc gcaggaggcg gacactaccg actacggcct gatggacacc ggcgaacgca
      481 tccagcgcaa ggacctgcag caggagatgc gcgagtccta tctggcgtat gccctctccg
      541 tgatcgtcga gcgcgccctg ccggacgtgc gcgacggtat gaagcccgtg catcgccgcg
      601 tgatctacgc gatgtacgac ggcggctacc gccccgatcg cggctacaac aagtgctccc
      661 gcgtggtcgg cgacgtgatg ggtaagtacc acccgcacgg cgacttcgcc atctacgaca
      721 ctttggtgcg tatggcccag tcctggtcga tgcgcaacat gctcgtcgac ggccagggca
      781 acttcggctc ccccggcgac gacccggccg ccgccatgcg ttacaccgaa tgccgtatgg
      841 ccccgctggc catggaaatg gtgcgcgaca tcgacaagga caccgtcgac ttcgtgccca
      901 actacgacgg caagacccag gagccgaccg tgctgccggc ccgcttcccg aacctgctcg
      961 tcaacggctc cgccggcatc gccgtcggca tggccaccaa catcccgccg cacaacatgc
     1021 gcgaggtggc ggacggcgtc cactgggcgc tcgaccaccc ggacgccagc cgcgaggaac
     1081 tgctggaaaa cctgatccgc atcatcaagg gaccggactt ccccaccggc gccaccatcc
     1141 tcggccacaa gggcatcgaa caggcctacc gcaccggccg tggcctgatc accatgcgtg
     1201 ccgtggtcaa cactgaggag atcaacggcc gtatgtgcct ggtggtcacc gaactgccct
     1261 accaggtcaa cccggaccgc ctcgtcgtct ccattcgtga ggccgtgcgc gacggcaaga
     1321 tccagggcat cgccgacatg cgcgacgaga cctccggccg taccggccag cgcctcgtgc
     1381 tcgtgctcaa gcgcgacgcc gtgccgaagg tggtcctgaa caacctgtac aagcactccc
     1441 agctgcagca gaccttcggn nnnnnnnnnc agaccttcgg cgccaacatg ctggcgctgg
     1501 tcgacggtgt gccgcgcacg ctgagcctgg acgccttcat ccgccattgg gtgaaccacc
     1561 agctcgacgt gatcgcccgc cgcaccgcgt acctgaagcg cgaggccgaa gagcgcgacc
     1621 acatcctgca gggctatctg aaggccctcg acatgctgga cgaggtcatc gccctcatcc
     1681 gtgcctccga agacaccgac accgcacgta cgggccttat ggggctgctg gacatcgacc
     1741 aggtgcaggc ggacgccatc ctggccatgc agctgcgcac gctgacccgc atgaaccgcg
     1801 acaagatcgt ggccgagcat gaggaattgc agcgcaagat cgccgactac atcgacatcc
     1861 tcgccaagcc ggaacgccag cgcaagatca tcggcgacga gctcgacgag atcgtcgcca
     1921 agtacggcga cgaccgccgc accaagatcc tgccgttctc cggcgagatg aacgtcgaag
     1981 acctgatcgc cgaagagaac gtggtggtca ccgtgaccca ctccggcttc attaagcgca
     2041 ccaaggccga cgaataccgc tcccagcacc gcggcggcaa gggcatcaag ggcaccaagc
     2101 tgcgcgagga cgacgtggtg gatcacttct tcctgacctc cacgcacaac tggctgctgt
     2161 tcttcacgaa caagggccgc gtgtaccgca tcaaggccta cgagctgccc gagggctcgc
     2221 gcgactccaa gggccagcac gtggccaacc tgctgcagtt cgcgcctgat gagtccatcc
     2281 agaccgtgct gtccatcccg aactacgagg tgtccaagta tctggtgctc gccacccgtt
     2341 ccggcaaggt caagaagacc ccgctggccg agtacgattc cccgcgccag ggcggcctga
     2401 tcgccgtgcg cctcatggcc gacgagaacg gcgagaacgc cgacgagctc atcggcgccg
     2461 cgctgtgcaa cgccgaggac gacatcatcc tggtctccaa actcggcatg agcctcaagt
     2521 tccaggccga cgacgagcag ctgcgcccga tgggccgcca gaccgccggc gtgcagggca
     2581 tgaagttccg taacgacgac agcctgctgg ccatggatgt ggtccctggc gactccgaca
     2641 aggacctgtt cgtggtcacc aacgagggct tcgccaagcg caccgccatc agcgaatacc
     2701 gtctgcaggg ccgcaatggc ctgggtatca aggccgtgca actggtcgag ggccgtggtg
     2761 ctctggtcgg cgcgctcgtg gtgtccgaag acgatcaggt tatggccatc atgaagtccg
     2821 gcaaggtgat tcgctccaac gtggacgaag tcaagcgcac cgggcgcaac acccagggcg
     2881 tgacctttgc caagcccgac aagggcgaca cgattctctc catcgcccgt aacgaggaga
     2941 aggacgagcc cgaggatggc ggcgatgccg cggcgaacgg caccgccgag accgcacaat
     3001 ccggcgagaa tgtgaacaac gcggacgagg cgtgagcagc gtcctgaaaa gctggtagcc
     3061 tcgcatacag cagaggctac cgccaagcct gttaaggaga aacgatgagc gagaatctcg
     3121 aagacaacga gccccggatc ataccgccgg cagtcggcaa tacgcagaac gagcatgtcg
     3181 agccgctgat cggtactgag cacgctcccc gcgtggcacg ctccgtgtcc agcgatccgg
     3241 aagaatccgc gttgccttct tcaggtgccg ccactcccgt gcgtccggca cctcgccgta
     3301 gcgcccccag ggctcgccgc atgagcttgt cgctgacccg cgtggatgct tggtccgtgg
     3361 ccaaggtgac gttcatgctg tccatcgccg tggcgattat tcaggtcgtt gcctccgcga
     3421 tcgtatgggt gctgcttgat gtggtcggtg tattcagcca gattacgcag attgtgtctt
     3481 ccaccggtct tgatgcaggc ggtttcgatc tcaagaacgt gttgtccctt accaccgtgg
     3541 tctccggcgt caccatcttc tcgattgtcg aagtggtgat cttcactctg ctggccacca
     3601 ttgcggcact gatctacaac gtggtcagct ctctggtcgg cggtgtgcac gttacgctcg
     3661 gcgacgactg atatagctat actgacggaa ctctcctcag tgaaccgcac cccggttgtt
     3721 ggactgaagt aattcagatt cgatgatcgg aggtgcggtt tcttatgtgt ggggatcgca
     3781 ggcgtcgttg ctgggccgag acttgggact ccggcgacgc tgggggtggc aggcgtcggt
     3841 gttgggacag attttgtatc tctgacgacg ctgtggaggg cttaccgtcg tcagcggtac
     3901 agatcttgga gttctgccga cgctgagggt gcgttctgtg tcgtatgcgt ccaattcttg
     3961 gccctctgcc gacgctgggc acagcaagcg tcgttgctgg gccaagtttt ggactgccgc
     4021 cgacgcagag aatgcacaca gtggtgttgg tctgccagcg tgtggggcgg attctagtgg
     4081 tggttgcaac acctgaggtt tgagcggcct tccatggtc
//
LOCUS       DAWBMW010000141         4117 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_4537_length_4117_cov_2.069670, whole genome shotgun sequence.
ACCESSION   DAWBMW010000141 DAWBMW010000000
VERSION     DAWBMW010000141.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 4117)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4117)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4117
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4537_length_4117_cov_2.069670"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1161..1170
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2345..2354
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3144..3153
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3583..3592
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          865 a         1229 c         1251 g          732 t
ORIGIN      
        1 tcgttatgag gccggaatgc ctggcgtgcg cttcggttcc gcttggcatg tccgcctcag
       61 tctctggtgc gaggagacga gacgtctctt tacgcggcgg caagccgaat agccaatgcg
      121 gtattcggtg cagcaaccat cgcaatagcc agccgaacct atgcgatagg ccgcgcggca
      181 taaaccatca gccggtaaaa ctgcgataca gttgagacca gttcaaccag ctgcatgaag
      241 gagtgccggc gatgatgttg tctcataatc cgatagtcga accatttgca ttggcccacg
      301 ccaccatcgt caccggagat aaagccggaa ccatactgcg taacatgacg atcgtggtcg
      361 gagccgacgg acgtatcgaa caggttgcac ccagcatcga aaccagcatc ccagccgaat
      421 accactatct tgacggcacc ggcaaaatcg tgatgccggg gctgatcaat gcgcataccc
      481 atctgttctc ccaaggcaag ccgctcaatc cgaagctggc gacgcccaaa ggccaacgta
      541 tggtcgccac attcgcgcat tcgccattgg gcaagccgta catggccgca accgtaaaac
      601 acaacgccac aacattgctg gaatccggcg ttacgacgat ccgcacgctt ggcgacgtgg
      661 gatatgaagt cgtgacatta cgcgatcaga ttgatgcagg ccaaatcctc ggaccgcgta
      721 tcttggcatc cggaccgctg atggcgattc ccgaaggcca tggcgccccg cttatcgcac
      781 tgaccagcgg caccccggaa gaggcgcgta ccgcagtcgc gcagaatctc aaggctggcg
      841 tcaacgccat caaaatcgcc gcgaccggcg gcgtaaccga tgcacaggaa atcggcgagg
      901 ccggcagccc gcagatgagc gtggaacaga tgcgggcgat ttgcgatgag gcgcatcagt
      961 acggtgttat tgtcggcgcg cacgcgcaaa gcccggaagg cgtgcggcgt tcgctgctgg
     1021 ccggcgtcga taccatcgaa cacggcagcg tgctcgacga cgaattgatc ggcatgttcc
     1081 ggcacaatcc gaatgcgttg cgcggatact cggcattgat tccgacactg tccgccggcc
     1141 tgccattgac cttgcttgga nnnnnnnnnn accggcatca ccgacatcca attggagaat
     1201 tccaagaatg tggtcggcgg catggtttca ggtgcccgtc aagcgcacga ggccggattg
     1261 atgataggcg tcggcaccga tacaggcatg acattcgtgc cgcagtacgc cacatggcgt
     1321 gagctggagc tgctggtcgc atacgccggt ttcagcccgg ccgaggccct gcacgcagtc
     1381 actgccgtca acgcctccat tctcggcgtc gatgccgaga ccggttcttt ggaagtcggc
     1441 aaatccgctg atttgcttgt gctgaatgcc aatccgctgg acgacctgcg cgcgttggaa
     1501 cacccggcac tggtcatcgc ggccggtcac ccggtatggc gtcccgctcc caagcgtttc
     1561 gcggatatcg acgccctgtt ggatgaggca tacgcctaat cgcggagtgc tgccgggccg
     1621 ttatgccggc aggtcattgt ccaacctgtg tggcgagatg ggggcatggc gaaaacagca
     1681 gcgaacgtac cggtaaccgt ggtgcccgga ggcgactcgt atttgaacga cgcgcgcacc
     1741 aaggcactgt gcgcacaagc gttgaaggcg cgacccgacg ccgaactcat cgagctcgac
     1801 gccacatcgg ccgaccaata tgccttcgat gaagccgtca gcccatcgtt gctttccgac
     1861 gttgccgtgg tcaaactcgt caacctgcag aatgccgacg aaaagctcgc cgaagctctg
     1921 gtgacgtaca ccaagcaggc cgccaaggat ccgaacggct ccagcgtggt gatctgccag
     1981 cacgagggcg gcgtcaaagg gcgcaagatc atcgaccaac tggtgaaagc cggcgctcgc
     2041 aaagaggacg tgccggattt gaagaaaccg gatgcccaac tcaatttcgt actcggagaa
     2101 ttcgagaaac gcggtcgtag agtcgaaccg atggccgcgc agcagctggt atccgtgctc
     2161 ggcggcaaaa ccggcgaact cgccgccatg tgcgggcaat tgtgtttcga ctttgacgat
     2221 aatccgatgg ggctcgaccg tgtcaaccag tatttgaccg ccaatccgca ggtcaccggt
     2281 tttgccgtgg cagacaaggc cgtggaaggc aaaaccgcag aagccatcgt catgatgcgt
     2341 gcagnnnnnn nnnngctgtg gaacaaggga ccgatcccat cgcgctgatc ggcgcgttgg
     2401 ccatgaagct ccgtaccatt gccaaagcct cggcggtccg ggccggcacg atttcacagg
     2461 ccgaagcgaa aaccaatccg tgggtgctga agaatgcgat gcgccaactt ggcggctgga
     2521 cttcggcagg actcgcgcat tgcatccgta tgctcgcctg ggcggatgag caaagcaaaa
     2581 cgaacggcgg cgacccggta tatgcgctgg aacgctgcat tgaagacatc agtcataaag
     2641 gtcggtaaaa gtcgataaag gggtatttat gagcgaacac actattgcgg ctcccaccag
     2701 cgaggccatg caggagctcg gccgccgcgt ggccggaatg gtacacggcg gcgatgtatt
     2761 gctgctctca ggccctcttg gtgccggcaa aaccacattc gcccagggct tcggcgcggg
     2821 gctggacatt accgaaccca tcgtgtctcc tacattcacc attgcccgcg aacttgacgg
     2881 gcatttcgcc gacggcactc cggctcatct ggtgcatgtg gatgcctacc gtctgggcgg
     2941 ctcagcctat gcgccggggc aggacgccat cgggcgtttg ctggacgagc tggaatcgct
     3001 gggattggac gaagaacttg aagaccccgg cgagaacacg gtggtgctta tggagtgggg
     3061 cgaacaaatg gccaccgcct tggcaccaga acgacttgaa atccatatcg atcgacctct
     3121 cgattcctcc gatgtcgcat ccgnnnnnnn nnnctcacca gcaacggcac tcgtaccgtc
     3181 gcattcgtcc cagtcggcaa gcgatgggcc gcgttcgacc tgcaataagc catgcgccca
     3241 tagactcctc gatgtacact ctctagaggc tttacgacta ggaggattca tgggttgcac
     3301 gttggtgatt gacacctcat tcggctcgac ggtcggcatt gtggggcgcg agcccattgt
     3361 agagaccgat tcgcgcaccc atgtggagaa actgcaggtc aatatcgcgc gcgcggtcga
     3421 ggaggccggc ctgactcctg ccgatatcga ggagatcgtc gtgggcatcg gcccggcgcc
     3481 attcaccggc ctgcgcgccg gcatcgtggc cgccaaggcg ttggccttcg ccaccggggc
     3541 caagctcgtc ggacagaacg tactcgaccc gcaaggcgag atnnnnnnnn nnggcgagat
     3601 gatgaacttc gtgctgggtg acagcgccat gttcgacggc atcgatttcc tggccgacgt
     3661 gccgcgcttg agttcgcgcc aatcggatgg tgacgtcccg gacgccgagg ccaaggaaca
     3721 cgaactcgac cgccacgtca cgctgtgcgt caacgatgcg cgccgcaagc agctgtactt
     3781 ctcgctcaac cacggcagca tcagcctgcc ggacgaggac gccgccgaac gacgctggat
     3841 cgagatggat atcgactacc cggagcatat cgtcgaacgg gtgaacgcgg cgctcgcgga
     3901 acacggcgaa cgcgacggcg tcagctatgt ggtggatgtg accggccacg gcgcggccaa
     3961 atacgcatcc gtctggcagg gactgcgtgc gctgggctcc gtggtggacg gatcggtatt
     4021 ggatgccggg aaagccgggc tcgccgtctt tgcgaccacc gcgttgagcc gcgaactgcg
     4081 cggcgaccag gtcgtgccga tcgaaccgtt gtacctg
//
LOCUS       DAWBMW010000142         4097 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_4566_length_4097_cov_2.663038, whole genome shotgun sequence.
ACCESSION   DAWBMW010000142 DAWBMW010000000
VERSION     DAWBMW010000142.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4097)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4097
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4566_length_4097_cov_2.663038"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    392..401
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2599..2608
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          789 a         1241 c         1255 g          792 t
ORIGIN      
        1 tgacatcacc cgtcagcaga ccgtggatat cgtgctgctg ggccgcttcc tggagcgtct
       61 cggcgatcac gccgtgtccg ccgcccgccg tgtggtgtac atcgtctccg gcttcgaccc
      121 cactaaggag ccgacccgcg acgagggcac cgacatcgac tgattgggac ttgttctcac
      181 attgggcgaa taactgaaca cttgttctcc tgtggtttga ggaggatgcc cgaaagcggc
      241 gatccggcta cggctggacg ccgttttttg ttgcttttgg gcggtacacc ggcgtatcca
      301 tggggcggca aaacacccat agggcggtaa cttgcccatg gggcggtttg aaggatatgt
      361 gaaaccgcgg aattaagcca ttctggcata cnnnnnnnnn ncatgagggt tttaccgccc
      421 catggggtaa tcaccgcccc atgggggatt tggcaggcat agcaaagggg agccgcacgt
      481 tatgtgcgac tccccttaca gcttgtaccg tgttagcggc ttaggctcac ttctggccct
      541 gagcggcgac agcggcggca ccggcagcag cagcctccgg gtccaggtac tcgccacgcg
      601 gcttgatcgg cttgaagttc tcgtccagct cgtagagcag cgggatggcg gtcgggatgt
      661 tgaccttggc gatctcttcc tcagacaggt tgtccagcat cttgacgatg gcgcggaggg
      721 agttgccgtg ggcagcaatc agcacggtct tgccggcctt gagctccggc tcgatagcgg
      781 actcgaagta cggcttcaca cgctcaacaa cgttggccag gcactcggct tccggaaccg
      841 gatcgccggc gtagcgcggg tcgttgttct gggcgtactg gtcgttcggg tcgatctcgg
      901 gcggcggggt ggcgtaagaa cggcgccaca gcatgaactt ctcgtcgccg tactcttcgc
      961 ggatctcggt cttgttcttg ccctgcagag cgccgtagtg acgctcgttg agacgccagt
     1021 cacgctgaac cggaatccac agacgatctg cggcatccag agcgatgttg gcggtgttga
     1081 tggcgcgacg cagcagggag gtgaacacga tgtccggaag gacgttcttc tccttgagca
     1141 gctcgccgcc acgcttggct tcggcttcac cctgctcggt cagcggtacg tcgacccagc
     1201 cggtgaactg gttagtttta ttccatgcgc tctgtccatg acggagcagt actagtttat
     1261 aagtcatacg gttcagtcta cagccgcgtg cgcccaaaca cgagtattcg tcttattatt
     1321 tcgctctcag agcgtcacgg aaaccgccag caccaccgca aacagcaagg tctggaagct
     1381 ggcggcgggc agagccgggc ggaattgacg tttattcacg ctggagacca tgcgcacagg
     1441 tattccgacg cccgagatca acagcactgc gccccacggc caccataggg tcgcgcacat
     1501 gagcagggct atggcccacg cggccacgca gcacacaatc agcagcgtac gcgcgccgga
     1561 ttcacccagc cgtacggcca gcgtgcactt accatgctca cgatcctcgt cgatgtcacg
     1621 gatattgttc accatcatga tcaggcagga gaacaggcca gccgccacag cacccaatag
     1681 tccgccgacg tccaccgtgc cgcacagcgc gtattcggtg cccaataccg cagccaagcc
     1741 gaagaacagg aacacgccga cttctcccag cccgacgtat ccatacggat gcttgccacc
     1801 ggtgtagaac cagccggcca gcaaggaggc cacacccacg gcaaacagcc accaagcttg
     1861 ggaaatcacg atagcggcaa tgccgcatat gcaggcgatg gcggcgcaaa tcatcgccgc
     1921 gaacagtaca tgcttggccg gtaccagtcc ggatgcggtc agtcgctggg gtttgcctga
     1981 tttcgattcg tccgtgtcac gcccggcgtc agtgccgcgg atgccatcgg aatagtcgtt
     2041 cgcatagttg acggcgattt gcaggaacag cgcgactagc aggcacagga tggtcaccgg
     2101 ccagaatcgg tccatcagca gggtctgctg ttcacgggcc gtggcgcagg aggccggttc
     2161 gggatatatt gcgatgcagg tgcccgactg ctcgatatgc gcgtatgcca tcgtcgcccc
     2221 aaccatcacc gacgcgatgg aagtggccaa ggtcttggga cgcgcgcccc gaacccacaa
     2281 gccgatggac ataagcaatc cctttcatat ataactcggc tcccctctct gaggggagcc
     2341 agagtatgcc taatcagcca acgagcggct tgaccagcgg gaaggtgatg gtctcgcgaa
     2401 tcgtggcacc ggtcaaggcg ataagcagtc gatcaatacc catacccata ccgccggccg
     2461 gaggcatgcc cacgccgaga gcctcgagga agtcctcatc gatgtcgcaa gcctcttcgt
     2521 cgccagcgag cgcgtccttg gcctgagcca cgaaacgttc acgctgcacg atcgggtcgt
     2581 tgagctcgga gtagccggnn nnnnnnnnta gccggtagcc agctcgaagc cgcgcacgta
     2641 gagatcccac ttctccacca cgccgggctt ggtgcggtgg cccttgacca gcggggaggt
     2701 ttcgaccggg aagtcacgca cgaaggtggg ctcgtaaagc ttgtcctcgt agaagtgctc
     2761 ccacaggtgt tcgacgagct tgccgtggtt ctccacgtcg tcgcgctcta cgccgagctt
     2821 gtcggcgatg gcgcccaggt gctcaacgga ggtgccgggg gcgtccgggc cgccattcgg
     2881 cacgatctcc tcgcccaacg attcggacag ggagtcgtac atgctgatgg tcttccattc
     2941 gccgccgaag tcgtattcgg tgccgtcgag cagggtgacc ttgtgcgagc cgtagacgtc
     3001 catggcggtc ttctgcacga gctccttgac cagctggccg atggaatcat aggtgccata
     3061 ggcctggtag gcctcgacca tggtgaattc gggggcgtgc gtggcgtcga cgccctcatt
     3121 gcggaagtca cggttgattt cgaacacgcg atcgatgccg ccgaccaggc aacgcttgag
     3181 gaacagttcc ggggcgatgc gaaggtacag gtcaaggtcg aaggtgcagg gccggcagcg
     3241 gctgcagggc cttagaggcg atagcccact cagtggcgaa tacggacagc tcgccggtct
     3301 tggaggcgat gacgcggccc ttgaggtaca ggtgatcgcc aaggtcgacg agctgcttga
     3361 actgcttgag cgagtcggcg ccaatctcct tcttggagat catgccctga atcttggtgc
     3421 cgtcaccggc ggacagctgc acgaagcaca ggccgcccgc gttgcgcagg aacagcacgc
     3481 ggccagcaat gccgaccatg tcttcggtct cttcgccagc ctcgagcttg ccgtcatact
     3541 tggcgcgaac cgcctcgatg gtgtcggtga cgtccaagtg caccgggtag ggggcaatgc
     3601 cggcctcaag catcatcgcg cgcttggcca cgcgcatctg cacctgctcg gggtgggcca
     3661 gcgggccgaa ctccttgttg gaggggtcca cggcttcgtc cagcgtggca ccggacttga
     3721 tcttctcgcg gatggtggcg tcctgcttga gcagcatttc cgcacgttcg acggtgctca
     3781 tctgcgggac ggccggggtt tcattctcag tgttttcggt attttcgaca acttcagcca
     3841 tgattcatca gtgtacgcaa ggccggcgat tatgagcccg gcagaccgca cggccatctg
     3901 aacacccgct cacatgacgc aaaaccggcc ctgcacaagg cttgagagac ctgtacacat
     3961 ggcgaatcca ccatcacgca acactcgcga catgccgatt tgcattgtcc accaccacaa
     4021 tgtaatatct acatctgttg ccaaacgggc tgtagcgcag cttggtagcg cgcttcgttc
     4081 gggacgaaga ggccgcg
//
LOCUS       DAWBMW010000143         4075 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_4599_length_4075_cov_4.208209, whole genome shotgun sequence.
ACCESSION   DAWBMW010000143 DAWBMW010000000
VERSION     DAWBMW010000143.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 4075)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4075)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4075
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4599_length_4075_cov_4.208209"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3613..3622
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          741 a         1144 c         1272 g          908 t
ORIGIN      
        1 cgtcgcttgc cggggttttg gcgagcgttc cggcgaattc cacggatacg tggccgttcg
       61 ggcgggtgaa ttggttgtag gtcatgccga acacgtcggt gaggttgtgc ggcgcgcggt
      121 cgtgccatac ggtcacctcg tcgtcggtta cgaaggagcg catggtggag acaaggtggc
      181 cgccgctgga gacgaattcg cgcagacggt cggttgtttc ctgcggagtg cagtacagtg
      241 ccggggtcac gatcatctcg tatttggcca ggcgttcggc cggcgcgtcg gacgggatga
      301 aatcgcattc cacgttgagt tcgaacagcg cgtcgtagat gttgcgcacc acgtcgttgt
      361 acttgagggt gccgccgaac gggaagccgg cttcgatgag gaaccagtcg agcgcggtca
      421 gcgattcgtt gctgaccatg atggccacct tgttgtgctt cttcaggtgg acgagccgct
      481 ctcccacttc cggtttggcg atctcgcggc cgaacacgcc cgcttcgcgg taggtgggat
      541 tcggttcgag gtcgtggctc agcaggccct tccagtatgt ttcgaaggag ttgtggatgg
      601 agtgccagtg ccaatattcc accatgtcgg cgccggaggc gaggtgggag taggcctgca
      661 ggcgcagctg gcccgggaac ggcacccagc cgtgctggcc ctgggcctcc gtttcgagca
      721 ccaggtagtt cctgccgtcc ttggtggagc gggtcatgtc gccgccgaag gcgatctcct
      781 tgccggtcag gtcgtcctcg gtggggtggt agatgtccac gccggtgatg tccacggcgg
      841 tcgaggcctt gaagtggtcg acggccggct gcacgccgta ggagtatccg cgccattcga
      901 agtcgaagtt gtgggtgatg aactggtcgt catgcgcgta ttcgcgcacg atgtcggcct
      961 gccactgcag gaaggcgcgc acctggtcgc ggcggaactt gtcgaattcg ccgcccaggg
     1021 actcgttgat ggtggcggtt acatcgggga agtcctccca ggagtcgatg cggttggacc
     1081 agtagtcgag gccgaagtgg tggttgagct cgtccagatc gccgtggaac ttctcgcgca
     1141 gatacttgac gaacaggcgc tgcatgtctt cggagacgga atcgtagtat ttcgtctcgt
     1201 tgtccacctg gtagccgatg acggccggat ggtcggcgac gtgggcgatg agcttgcgga
     1261 tcacgcgttc gccgtagaaa cggtaggtgg cgttgacgat gtccatgatc tggcgtgcgc
     1321 cgtatttgcc ttggccgctc ggggtgaccg cgagcacgct cgggtcgagt ttgacgagcc
     1381 agctgggcac cgcgtaggtc ggcgtgccga cgatgacgtc gatgccggcg cggttggctg
     1441 cgtcgagcgc gcggtccaca taagtgaagt cgaatacgcc cggttgcggt tcgcaggtgc
     1501 tccatgtgga ttcggcgatg cggatgacgt tcagtcccgc agccttcatc atcgccatgt
     1561 cctcatcgat gcggtcaatg cccttcatca tgagatattc gtcgtaatat gcggcgccga
     1621 acagaatgtg gtctgcctcg taacggccca tgaatcctcc atcgtcctcg ttgactttta
     1681 tatctaacgg ttattagata taagaactat atatcttcct atcccaaacg tcaaataccg
     1741 cagaatttca ggcgtttctt aactaacagc cattagatac gcacgtcacg ggacggtcta
     1801 ccggtatccg tcccacaccg gcgaggggaa gatcatccgt tcgaaggcga gccactcgtc
     1861 gtacaggtcg atcggcggct tgcgagtcca ccgcaactgg atgccatcca tcgcctcgat
     1921 gcattgccgc acgatcggcc gcatattatc ccatccgccg acttccggag gcagtttcca
     1981 tgcgaacttt gagtaatgct cccacgtcag atccggtcgg ttttcgaaat actcgtgcag
     2041 tggatggtcc gcgttgaacg attcggtttc caaaaccatg tacagctgca gcagactgcg
     2101 gcgttgcgca ttgtagcgga ccagaaaccg cagatatgcg ggaaacagca tgccgtcaga
     2161 atcgctgccg ggcaatcccg acttagcgaa gtcggcgggt gtgccctgct ggtcgtagct
     2221 atcggtgacc agcagcgaca acaaccgctc cttgttgccg atgtagtgca gcagtccggg
     2281 ctggctcatg ccgatggcgt cggcgatgtc cttgagcgac gtaccgtagt accccttctc
     2341 gccgatcagc ttgcccgccg ccgcgatgat ttcgcgtttg cgctcttcgg gcgatttacg
     2401 aacccgcttt ttcccctcgg taaccatatg gaacagcgta ccatcggacg ggccctcggc
     2461 cggtcacgcc tggaagacgt ccggatgctt cgggtcgccc ggttcgtcgg cgagcacgcc
     2521 ttcgagtccc aggaactcct gccagaattc caacgaattg ccgtatggga tggcctggct
     2581 tccgagcgcc tgcaggttcg ccttggcctc gaacacgtcc tcctcgcgga tgccgtcgaa
     2641 gtagctttcc agacgttcga gattcgtcgg atcgtagaac agcgagccga tgatttcagt
     2701 aagccgctcg gcgcgggcca ccggcagcga atcccagtgg cggaattcga tgaagttctt
     2761 caaacgcacg tcgttgaagt gcgtggagat cacatggttg atctcgtacg cgttgagctc
     2821 gcggtccggg tacacgtcgt tcgcgttctc gtagaacgcc ggatggtgca aatcggttcc
     2881 gggcaccgcc agcgcttcgg gcgtgtgcgt caggtcggcg aacatcatcg gcgtggacag
     2941 cacgtccacc gcgtaatcct cccaaccgaa acgcgggtcg aacaggccgg ggatgacgtt
     3001 cgtgcgttgg aaatcgagat aatcccacat gcgttggcgc agcagcggat acggattctc
     3061 cctgccttcg aaatacgggg tgttgcggaa gaaccatgcc agaatcgggc cgatcaccgt
     3121 gcccaaccgc agtttggcga tcgcgtcctg ttccgaaacg tagtcgatgc tgacctgtgt
     3181 cgaagcggag caacgcatca tgcacggacc gaactgtccg acacgtccca ggtaggcggt
     3241 catcgccttg tatcggtcct tcggattgac gggaatgtcg gcgtaactgg atttcggttg
     3301 gtaaccatag ttgacgagtc ggaaaccaag ttcttcgagg atggggtcga cttcgcggcg
     3361 gaacgcgccg tacaatgtgg cgagctcctc cggcttgtgc aggatgccga tggacgtttc
     3421 caactgtccg cccggttcca aagatacgga gatgccgtca cgggcgagtc cgaccagtcg
     3481 cccgttttcc cagtattcct tgttttcgtc gtaatacggg cggatgcggt tgagcaacgc
     3541 ttcaatgccg ttgctttcgt agtaggtcac cgcggtatcg tcgctgttat gtaccggcag
     3601 gtgttcgatt tcnnnnnnnn nncgatttcc acgccaaaac cgccggtgcc gcgctgttgg
     3661 cagccggatt cgaagaattt gataaggctt tccacatgct tcggattcgg ctcggtcagc
     3721 aggtgcgcgt acgtgatctt tggcgttgtc atagtggcca tcctaacgaa aagcgccatt
     3781 cgcaacgcag gctacgctat acggaaaatc gataaccgac catcattccc catcgatttg
     3841 gctggcgatt tgaatgccga ttcgacgacc atcgatttta cgatcgccga atcagccgtc
     3901 gatttggcgg ctcagatacc gtcgaaccga cgtcggaaag tttcccaacg tcggttcgac
     3961 ggaaataaga atggcgattc aagcggcctt ctcgctgttg cgcgcctctt cggctgcgcg
     4021 gtgagccttc ttggcggctg cgatcatcag tttgatgcgg ttgagctggt tcgct
//
LOCUS       DAWBMW010000144         4029 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_4648_length_4029_cov_2.512079, whole genome shotgun sequence.
ACCESSION   DAWBMW010000144 DAWBMW010000000
VERSION     DAWBMW010000144.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4029)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4029
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4648_length_4029_cov_2.512079"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3700..3709
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          776 a         1277 c         1150 g          816 t
ORIGIN      
        1 acatcgaacg catcgtttca gacgacggat acggcaagaa cgagtacatc gagaccaccc
       61 ggccgctcgt cgtggtcacc gcgcccggcc ctggctccgg caaactcgcc acgtgcctga
      121 gccagctcta ccacgagcac cagcgcggca tcgacgccgg ctatgccaaa tacgagacgt
      181 tccccatctg gaatcttccg ctcaaccatc cggtcaacat cgcgtacgag gctgccaccg
      241 tcgacctcga cgatgccaat atcatcgacc cgttccatct ggaggcgtac ggcgagacca
      301 ccgtcaacta caaccgcgac gtcgaagcat tccccgtgct caaagccatg atggagcgca
      361 tcatgggcga aagcccctat cagagcccca ccgatatggg cgtcaacatg gccggatacg
      421 ccattgtgga cgacgatgcc tgccgcgatg ccgcgcgtct ggaaatcgtg cgccgctact
      481 tcgccgccgc cgtgcacttg aagcgcaccg gcactggcga ggaacaggtg gagcggctgc
      541 gctccatcat gaaccgggcc ggcgtgaccc ccgatctttc tcccgcccgc gccgtggccc
      601 ttgagaagga agccgccacc ggtgccccgg ctggtgccat ggtgctgccc gatggccatg
      661 tggtcaccgg caaaaccggc gacctgttgg gcgcggccag cgcgctgctg atgcacgcgc
      721 tcaaggccgt caccggtgtt gacgaaacca ttccggtaat cgacgatgcc gccatcgaac
      781 ccatctgtcg gctgaaaacc gagcatctca acagcgtgaa ccgacgcttg cactcggatg
      841 agacgctgat tgcattgtca atcaccagcg ccaccagtcc tgtggccgct cgcgtcatcg
      901 acgggctcaa acagttgcgg ggctgcgacg ccttcttctc cgttatcatc tcatccacgg
      961 acgaggcgct gtatcgcaaa ctcggcatca acgtgtgctg cgaaccgaag tacgaacggg
     1021 tgagtctgta tcaccggtaa gcgccaccga taagcgcggt atgccgcgaa tcgtcaagcc
     1081 ggtaatcatg cagcatgcgg cctgacgatt cgctgctgtc gcattgtctc tcccctcggc
     1141 catgctgggc ttgttttccg tttgttggag agaacgggga atgccatcag cactacggaa
     1201 agagaaggat tatccgtgaa gcggttcgtg ggtttagacg gtattaaagg actagcgctg
     1261 atagccattg tgctgtacca ctgcatgcag caacggctgc caggcggatt ctacggagtc
     1321 gacgtgttct tcaccgtctc tggattcctg attgccgtca gtttgattcg ttctctggcg
     1381 aaaaccggat cgctgaatct cgtgcgatat attcccaagc gtgcagtacg tctctatccg
     1441 gcgctgtttc tgctgattcc ggtcatcgtc tccgtcggct ggctgttcga tcacgacatt
     1501 ctagtttcca tccgcgacca agtcatcacc gtgctgctgg gctgctacaa ctggtacgcc
     1561 attgccggag gccaaagcta tttcgaccaa atgaacccgc aggtgtttcg ccatctgtgg
     1621 ttcattggcg tgctcatgca gttctacgtc atcgtgccgt tcatcgtctg gctgatgtgg
     1681 aagctccgcc agacgaaact gtcttcactc atcccactgg gactcgcggc gttcagcagt
     1741 atcgccatgt gggtgatgta tgtgcccaaa ggcgacccca cccgcgtcta cttcggcacc
     1801 gatacccaca gcatgggcct gatgctgggt gtggctctcg cctggtgggt caccgctcct
     1861 cagttgggcc acgctcgcca gagggctgtg ccgatgcccc gcaccattag ctcgagcgct
     1921 tcggcagccg cacatcgggc tccgttacct cagattcccg tggagcatcg catctggaat
     1981 gcggcggcac cggtattggc gttcctcagc ctgattgcct tggtcaccat ggcggttatc
     2041 ggcaaacagg atgcattcgc cttccgcggt ggcatcatat tgtccagctt gttgtccgtg
     2101 ctgctgattg ccggtaccat ctccgacgat tcctggatgc aaagcctcat ggtgttcaag
     2161 ccattggcct ccctcggcaa gtattcgtac ggcatctacc tgtggcactg gccgttgtgg
     2221 attctttcca gcgcccttgc cccgaagatc ttcaaggcag tagggccttg gcctctcatc
     2281 atgaccgcgt tgctcaccgc cattgcggtg atgatttcct ggctgatggt ggagaaaccg
     2341 gcggcaacgc actcggcggt atccgtcgtg gtgccccttc gcacgaacaa gaagaatcat
     2401 gtggcccggg ttattgccgt tgacgttatc ctggtgctgt cgttggccgg ctgcgtgcag
     2461 ggcattgctc atgcacctgc gaaaaccgcc atgcagatcc agctggagca acaggccgca
     2521 caactggaac gtatgcagaa aaccgaacac gtgctgctgc gccatgccgt gccggcaccg
     2581 cccaagccca agcatgagat gcccaccggc gatcagatta ccgccatagg cgattcggtg
     2641 atgctggcct catcacaggg cctttcggaa gtcttccccg gcattcagac cgacgcgagc
     2701 gtttcccgct ccatcatggt cgcccccgaa ctcatcggca acgctctgtc cgccggcagt
     2761 ctgcggcagt gggtgttgat cggccttggc accaattcgg caatcacccc tgatcagttg
     2821 gaccagattc gtaatatgat cggccccgac cgcgtgctgg tactggtcaa tgcccacgga
     2881 gaccgtacgt ggattccacc taccaaccag gtgcttgccg actatgcggc cgcacatcca
     2941 gacaatgtgg tgctggtgga ttgggatgcc acagccaacg cgaacgcgca ggtgctcggc
     3001 tccgacggca tccacccgag catggatagc gatatctatg caaaggcggt caaacaggcc
     3061 atcgaacaat ggatcgcaag cggcaggtga acggcaggca acgctgagtt gtccaaccag
     3121 tcgtgaagaa cggctatgct gaagactgca tcaacgagac cacacgttgg gagacatggt
     3181 gagcgcaatc cttgaaggga agcccgataa gaatcttatt ctcgttacgg gaaggattca
     3241 tccgaagttg gctgaggatg tcgccgagca gctcggcatc gacgtccttg aaaccaccgc
     3301 atacgatttc gccaacggcg agatgtacgt gcgctacact gaatcggtgc gtggcgccga
     3361 tgtgttcgtg ctgcaaagcc actacaagcc catcaacaag gcgatcatgg agcagctcat
     3421 catgatcgac gcattgaagc gcgcctcggc ccgttccatt actgcggtct gcccgttgct
     3481 cggctactcc cgtcaggaca agaagcaccg cggccgcgag cccatctcct gccgtctggt
     3541 gttcgacctg ctgaagaccg ccggtgccga ccgcatcatg tcggtcgatc tgcatgccgc
     3601 gcagtcccaa ggcttcttcg acggcccggt cgatcatctg gtggccatgc cagtgctggt
     3661 cgactacatc cgtgatcgtt tccagggcca tctcgacaan nnnnnnnnnt cgccgtcgtc
     3721 tcccccgacg ccggccgcat tcgcgtggcc gagcagtggg cgcagcgtct cggcggcggt
     3781 ccgctcgctt tcgtgcacaa gacgcgcgac atcacccgcc cgaaccaggc ggtcgccaac
     3841 cgtgtggtcg gcgacgtcgc cggcaaggac tgcgtgcttg tcgatgacct gatcgacacc
     3901 gccggtacca tcgcgggcgc atgccacgtg ctgcaggacg cgggagccaa gtcggtgacc
     3961 gtggtcgcca cgcatggcgt gctctccggc ccggccattg accggatcaa cgacagcgtc
     4021 atcaccgag
//
LOCUS       DAWBMW010000145         4010 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_4675_length_4010_cov_2.270544, whole genome shotgun sequence.
ACCESSION   DAWBMW010000145 DAWBMW010000000
VERSION     DAWBMW010000145.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 4010)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4010)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4010
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4675_length_4010_cov_2.270544"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1829..1838
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          778 a         1188 c         1189 g          845 t
ORIGIN      
        1 ctttgattcc gattacctgc ccgcgcatgg tgcacttcat gggcaaagcc gaatacttcg
       61 agggcaaggg attgaagggc aagttcaaga agtggtggtt cacgtccgtg ggcgtgttcc
      121 cggttgatcg ttccggtggc agcaagtcgc ttggcgcact caagcacgca cgcgagatta
      181 tcgaggatgg gcacctgttc ggtatccaca ttgaaggcac gcgcagcccg gacggtcgac
      241 tgtacaaggg acacaccggt gccgcacgac ttgcgctcga gaccggttgc ccgattgtgc
      301 cgaccgccat catcggctcg gacaagctgc agccgattgg cacgtcgatt cccaagaagg
      361 gcaagaccaa ggtgttgtat ggcaagccga ttgaagttgc caagaagccg gccgatgaga
      421 ttacacatga tgatttgcgt tcgctgaccg atcgtgtttc cgccgcaatc cagaaaatga
      481 gcggtcagga attcgtgaac gaatacgccc agaaggtcaa ggccgagctc aaggcccaac
      541 aggccgccga agaagccgaa taatatgtgt tggctcccct cagcgaggga agccgttgat
      601 tactctcaca cgatggtgag ggtgatgacg gtggggttgc cgttggcatc gcgcaggcga
      661 atcttgccgc cgttggatag atccgtatcc ccgcttttca cggtacgtac cgtgccgaac
      721 agaccaccga gcaccttgtc ggttttcacc tcgaacccga gaccctcaag cgtcttggtc
      781 acggcgcttt cctgtttgcc gacaaggccg gacggtatcg tcgccatctc cggacccttg
      841 gaaatcacca cgtccacgct atcgccccac ttcagctggg ctccggcttc ctgagacgcg
      901 gaaatgactt gaccggcctc gaccttgtca tcgtactgtt cggtgacatt ggccgtgagc
      961 ttcaactcgc cgagcgcggc ttgcatctca tcctgggtct taccgacgat gttaggcatg
     1021 gtcaccggca tcggtccctt gctcaaagta accgtcacct catcgttgtg cttggccgtg
     1081 gtaccgggat caggggagat cgtcaacgcc acaccctgcg gcgtgtccat cgaatactcg
     1141 tccttcgact cgtcgtgctt gacattatcg aagccagacc tcttgagcgc gttcaacggg
     1201 tctttgccat tggccgaatc cgcgtcgaga atatccttcg ggatggtggc catcctcacg
     1261 cccttggaca ccaccaccgt aagctcctga ttggctcgct tgcttatgtg gctgttcaca
     1321 acggccttgg tcttgttcac ggaagaagag atgatcttgc cttccgccac gtcgtcgctg
     1381 tattccttgt gcgtcttgta ggggatgccg agagccttca acgtgctctc atacgtggcc
     1441 caatccgcgc cggccaacga gcattcggtg cttgcattcg catcgcaggt cacgtcatcc
     1501 ggcttgggca cactccaata gcttcccggt ccggcaaacc accaccagcc ggcggttccg
     1561 ccgattgcag ccagtaccag cacgatcacc agtacgatga tgagcggttt tttgctgcgc
     1621 tgcttctttg acccggtgcc attggaagcg acgtccggct catcggaaag ggcaagcgct
     1681 cgggtgaagg catcgccggc ctgaggcagc acttgtgtct cgctgccgga ctgtgccatg
     1741 atctgcgtct tatggggagc cgaagcatcg gctaacatcg acgaatcaag tgccgtggtg
     1801 ggggcgaccg gaggcgccgg gggattgann nnnnnnnngc ggtattgctt gcggcatcat
     1861 cggccaaaaa tgccggtacg gacggctcga acgttgctgc accggcaggt acgccggtca
     1921 gcggggcctg ctcggcgata ttgcctacca gcgctgcagc ggtggcatcg gtatgatcac
     1981 ctccgatggg ttcggcatgc aaacggtatt gccacgcctc cagcggcaat ttggcggcca
     2041 actggctcaa ttcctcagct gctgccgcgc catcggcggg gcgggcatcc acctgacgcg
     2101 cggtcagatg ggcgatgaac gccgccacgg acggatcaat gccctggcag accgtggcca
     2161 cggacggcac gtcctcatga acatgcttga agacaagcgt caccggatta tccgaatcga
     2221 acggcacctt gccggtcagc atctcccatg ccatgatgcc caccgagtac aggtcgcctt
     2281 gcgcggtggc ctgattgttc tcgatcattt cgggagccag ataagcggcg gtgcccaata
     2341 gcatgccggt ggagctcaat gtggcctgac tggcggcctt ggccagaccg aaatcagtga
     2401 tctgcacatg gccgcgatca ttgatcaaaa tgttctcggg cttgatgtca cggtggacca
     2461 cgccggcgcg atgcgcggaa gccagaccat ccagcgtctc cgcgaccacg cgcagcgtct
     2521 cacgtacgga gaatgtgcct tgggcgttca tctcatgccg cagattgacg ccatgcacat
     2581 attccatgac cagaaaatca agtccattga actcgccggt gtcatacacc tgaacgatgt
     2641 gcgggttggc gatggacgcg gccgaattgg cctcgcggcg gaaccgttcg acgaactgct
     2701 cccgatgcgg gcctttggcg agttgtgtgt gcatgacctt aatcgccacg gtgcgcccca
     2761 gacgctcatc gacggcctca tacacggtgg ccataccacc ctcggcgata ttgcgcacga
     2821 tgcggtagcg tccctcaatc atctgcgcgg gctcgttctc actcatgttg tatcgtacgg
     2881 ttcctttaat cacgactaaa catgttcagt gtacatggcc ccggttacgc ggccggagca
     2941 tcagcgcata gttttcacgg gttttcaccg gtgttcatgg tctcatggtc cggcggctcg
     3001 cattgtggac tatcgcaagg attcggggat gaatcgcgca caagcctcgg tcagacggcg
     3061 ttgcgcttcg gaattcagct caagaccggc aatcgcctta cgggactcac cccatagggc
     3121 agcgatgcgc tcgcgggatc gatcaagagc accggtcgac gtgaacagtt caatggtgcg
     3181 ggccacctgg ttgtcattgc gacaatccgc ttcgaagatg gcaatcagat cggccttatc
     3241 cgccgtatct gccgcagaca gagcatcagc caacagcact gtgcgtttgc cttctcggat
     3301 gtctccgcca attggttttc cggtattgcg gcttgatccg acgacatcca acaggtcatc
     3361 cgccagttgg aacgccagtc cgagcggccg gccgacggcc aacgcgcaat gtctggcctg
     3421 atccgggctc tctcctgcgg ccagcagtgc aagcagcagt ggagcgatgg tggtgtaact
     3481 ggctgtcttc cagcggaata cgttcaatga ggcgttcgcc agagcttcgg gattggacag
     3541 tggggtcatc tcgacggcca agtccagtac ctggccgatt tccacctcgc gttgcatggt
     3601 aagaaacgcc tcgttcaggg catcggtgtt gggaagacgg ctggcggatc ggcgtgtgat
     3661 ttcaatgcag gcggtggcca gaatgtcgcc gagcatcagg cccaagccat ggcctatggc
     3721 accgctgtgt acggcctgtt ccaaggcatg atgtgcggaa ggcttgcccc ggcgcagatc
     3781 ggattcgtcg atgatgtcgt catgcaccag tgcggccgtc tgaaacactt caatggcgca
     3841 ggccaggtcg atcatggcgt cacgccgatc aggagtcacg tcgccggcaa gaatatcgaa
     3901 ggcgtccaat gcgagcagcg cacgcagtcg tttgcctcct tggctggagg aggaggcttg
     3961 gtccacaacc tgttccatca ccggtttcag ggcgtcggca acctcatcgg
//
LOCUS       DAWBMW010000146         3969 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_4745_length_3969_cov_2.060807, whole genome shotgun sequence.
ACCESSION   DAWBMW010000146 DAWBMW010000000
VERSION     DAWBMW010000146.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3969)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4745_length_3969_cov_2.060807"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          841 a         1138 c         1119 g          871 t
ORIGIN      
        1 tcatcatatc aaaattgtca tggatcttgt cgttaaccat acctctgatg agcatgcatg
       61 gtttatcgaa agccgcagtt ccaaagacaa cccttatcgt gattactaca tctggaaaga
      121 cccgaagaat ggaaaagagc cgaacaactg gggtgcctgc ttcggcggtt ccgcatggga
      181 gtcgtgtgga gacggctggt actacctgca tctgttcgcc aaggagcagc cggacctcaa
      241 ttgggacaat cccgaggtgc ggcatgagtt cctgcgtgta ctcacattct ggtgcgatcg
      301 cggtgtggac ggtttccgca tcgatgtctc gcacggtctg gccaaagacc tgcgtgagcc
      361 gctgcgcgac cgtatcgacc cgacactgat gtctccacag gctaccgatg gctccgatcc
      421 gctctgggac cgcgacgccg tgcatgatat ctaccgtgaa tggcgcgacc tgttcaatca
      481 gtacacgcca gccaagtatg cggtcggcga atcgtggtcg ccattcacca cccgtatttt
      541 ccaatacgcc aagcctgatg aacttggtgc cgtattcgac ttctccatgt caaaggccgc
      601 ctggaatcgc gaagaatacc gcaccacgat tgagcgcacg catcgctatg ctctcgctgc
      661 cgaaaccgcc cccacctggg tgctcggcaa ccacgatgtg ccacgtatcg cctcgcgtct
      721 cggactgccg agcggtgcaa acatcgagca gtgggtgacg tccaacggca cgaatccgcc
      781 gattgacccc gcgctcgccg ctcgtcgtgc ccgtgcagca gcgctggtga tgctcggcct
      841 gccaggtacc gcctttgtgt atcagggtga agagcttggt ttgccggagg actttgattt
      901 gaccgaagat gaaattcagg atcccaattg ggaacgttcc ggtcattatt tcaaggggcg
      961 cgacggttgc cgcgtaccgc tgccgtggca gtcagacggc ccggcgttcg gcttcaatgc
     1021 caccggtgct tcgtggctgc cccagccggc atggtttgcg cagtatgcca ctaatcgtca
     1081 gattggtgac ggggcttccg tcctgcacct gtattgtgct gctgtcgcat tgcgcaaaca
     1141 gtgggtggcc aacggcacgg acttccagtt ctcgtggctt accaacgaag agtgtccggc
     1201 gccgctgctt ggttggcgct tgccgtccgg catggttgtg atggcgaact tctctgacag
     1261 tcaaccagtc gccttgctgc agccgatgac cgtgctactt gtgtctgacg cgactgtgca
     1321 ggcgaacact caggtcgaga gcgttcctcc agctgctacc gtctgggcct tgccagcata
     1381 atctcattga agaacaccaa ggagttacgc caatgattgc cgaaacctgt ccaataaccg
     1441 ttcgcacggt aacggcccat cttgataccg gtcgtgccgt ctatggcgaa caggcttcgg
     1501 caatctgtga actgccggta gaagccgtca caatcgttct cgaatcatgc gaagacgagt
     1561 gcacgtccag tgcgggtatc cagggtgatg ctcaggtgcg actctggcgt atcgatgtga
     1621 acgcgcatgc accccagccc aaggaccgcg ccgctgcctt tcaggaacaa caagcgtttg
     1681 agccccacca tgcgattgac gtgtcgctca ccatggccgg cgtcgacagc ggcaacgatg
     1741 acaccatgct ggtgctatac cagcacaagg agtggtggat gcgcccgacc tgggtgcgta
     1801 caccatctga actgcctgaa cgcacgcagt tattgttacg ccgcaacaac gatgccgaag
     1861 atgccgagtg gctagtgttg gtcgctatct gcggcacgga tattcgggct gatttcagtg
     1921 ggcagcccgc aacagaatct gatgacacgg ctttgcggct cgtgctgtct tccaaccggg
     1981 ttggtcgcac gactctatgt gacactgccg catatatcgc ttgcgcatcc gacccgtata
     2041 tggccattcg agctgccaca caaacggcag cacgccagct gggtattcgc acccgtaagg
     2101 agcgtccatt cccggatgcg ctcaccgggt tgggatggtg tacttgggat tcattggggc
     2161 gagatgtcaa cgagcaggct atcgtcaaca agatggagga gttccaagcc aagcatgtgc
     2221 ccatttcctg ggtgcttatc gatgacggtt ggtccaacac ggatcgcaca aaggaaaccc
     2281 tgatcgactt tggcgcggat cgccaacgtt tcccccacgg gcttgcccac accattgcgc
     2341 tgctcaaaac gcactatggc gtgcgcagcg taggcgtgtg gcaggcgttc caaggctatt
     2401 ggaatggttt ggacgaatcg ggtgtagccg cggccagctg cccgaccgca ataaccacca
     2461 cggcgaatgg ttgcctgatt cctggaagtc gtgctgaaca acccgcacag ttctgggatg
     2521 cctgggacgg cgaactcgcc gaagccggcg tcgatttcgt caaagtggat tcgcaaagtt
     2581 ccacgtcagt aatggttcgt ggcaccgaaa gttacggtga agccacctgg ggacgccatc
     2641 aggcgcttga tgaagtgacc tcacgacgat tcggcggcgc attgattaac tgcatgggca
     2701 tggcaccgga ggattattgg catcgtcctt catcgccaat cacccgtagt agcgacgact
     2761 atctaccgca caatcctgat tcgctgggcg aacaccttat ccagaacgca tactgtgcac
     2821 tgctgatggg ggagctgtac cactgtgatt gggacatgtt ctggacggag catccccatg
     2881 cgcgagtcca tgccgtgctc cgattgctta gcggcggacc agtgtattgt tccgatgcct
     2941 gcggtcacac tgatgcggct gtcttgcgtg atttacttgc cgaagatggt acattaccgc
     3001 gtcctgatga gccggcccga ccagtcattg cgtcactgct caatgacccc gaacatacgg
     3061 actatgcgct cggtgtcaca gcaagattcg gcgcagaaca ggtcatcgct ttcgtcggat
     3121 tgcgccggca gccacaaatc ggcattatta cggctagcgg tcgctcttgc cgcatcatcg
     3181 atgaccatgg tgcgacgatt gatatgaatc cgggagaaac ccatacttgc gagatcgagt
     3241 acggccgact gcgtgttttc cgggtgcaat catgaaagcg catatgccga ttgatattta
     3301 ttttccggca caccaacgca gttctccaat gggaatgtaa ttcgccaatt tttagactgt
     3361 gagcaacgag caatatagat atcaataagg agccaatgat gactactttc aaccgcgcaa
     3421 taatccccga cgccatccgc accaacggcg ccacccccaa tccgtggtgg tccaacgcgg
     3481 tggtctatca gatttacccg cgctctttcc aagacacgaa cggcgatgga ttcggcgatc
     3541 ttaagggcat cacgtcgcgt cttgattact tagctgatct tggcgtggat gtgctgtggc
     3601 tatccccggt ctataagtct ccgcaagacg acaacggcta tgacatttcc gactatcagg
     3661 acattgatcc tctgttcggc acgctcgacg atatggacga gctgctggcc gaggcccaca
     3721 agcgtgggct caagatcgtg atggatcttg ttgtcaatca cacttccgat gaacacgcct
     3781 ggtttgaagc atccaagaac aaagacgacg aacacgccga ctggtattgg tggcgtccag
     3841 ccaaaccggg gcatgagccg ggcactccgg gtgccgagcc gaaccagtgg ggttcctatt
     3901 tcggcggttc cgcttgggag tacgatccga agcgcggcga gtatttcttc caccagtatt
     3961 ccaagaagc
//
LOCUS       DAWBMW010000147         3933 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_4806_length_3933_cov_2.515214, whole genome shotgun sequence.
ACCESSION   DAWBMW010000147 DAWBMW010000000
VERSION     DAWBMW010000147.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 3933)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3933)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3933
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4806_length_3933_cov_2.515214"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3641..3650
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          874 a         1150 c         1103 g          796 t
ORIGIN      
        1 tcgccaaaga caatgaagac gccgccgcat atcaccacgt ccagagccca gagcaggcca
       61 accaccaaca gaaaaaggta ccgacctaca acttgtgcag tgcgtgaaac aggaatcagc
      121 cctcgcatgg cactgtgccc tgcagattcc tcggatgacg caggccccaa tgcactcata
      181 tatgccatca ccgatgccag acctccgagt ccgcccatca gaccactggc aacgtgttca
      241 tcggcgtggg tcgatatcat caccagcgat accacgatag acagcaccgg gaaaaccagc
      301 agaaatacca tattcgccac gctaaacaga cccgtcccga cacaatcgaa tcgacattgc
      361 tttactacgg atttccagct catcgtcacc attcccttga agcaaatatg cgagaggcaa
      421 ttacatacga gattccatat gccacaaccg tcgttgtcaa taccgcaata accagccaca
      481 atcgttgcgc gatgatggca ttcgcggcat gggccaatcc tctggtcacc gaagcaggca
      541 aaatagatgg cagtatgaat acagccaata caacgacata aatcaccatc atcgtgactc
      601 gaagaccgcc tagtttggtc cacagataac ccgtgggcac gataagcgcg accatcagcg
      661 cggtgcagaa acctcccaac ggcgccgccc acagattgcc cgcgaatgac caatccggat
      721 tgaccagcac ttgcaacccg tcaatcagaa caatcatcgc tatcgacaat acggaaatca
      781 ccaatccaac cacatatcgc gcagccacct gactcttcct cgaagccgga ataatgccac
      841 gaagtcgata tccgttctgt aagtcagcaa ccgccatggt tagcacccac atggcgcaga
      901 tgaataccgt catgatgaca cccatgcgat agccgaagcc atcaccataa gcttcacctt
      961 gaaagatgac ggagaacaac ggtgccaaca acaacgagaa cacagtccgt acgtcgacgc
     1021 acaggtaggc catatcgacg cgcaatgcag tggtcaatgc atgttgccga gaccgcgaca
     1081 ccctcagtat tgcctccatg gccgctcacc tcccgctcaa gtcacgattg gaatcatggg
     1141 cattggtcag tcgaatgatg tcatcaatgg aagccgattc aacgacaagc cccgaaacca
     1201 ccgacgcaac agcatcaagc ctgtctgacc ggaccagcgc atcgaatccg gtggcatacg
     1261 tatgtgagcc cagcactacg tccgcaggca gttgtgccag ttcgtctgga ccgcccttaa
     1321 tcaggcggaa cgattcctca aactcgtctt tcggaccggt gtagtagaga cggccatcgg
     1381 tgatgtaggt aatgaagtca gcagcacgtt cgaggtcggc agtgatatgg gtgctgaaca
     1441 gtaccgaatg ctcgccatcc tcaatgtagg catgcaggat atccatgagt tcgtcgcggg
     1501 caagcacatc gagtccgctg gtcggctcgt cgagaatcag cagcttggca tcatgactca
     1561 atgcgatggc cagcatcagc ttcatctgca tgccgcggga aagatccttg atatgtttct
     1621 tgccgttctg tgcgtcacca aggccaaaag tatccagata cttgcgatag catccaccgt
     1681 cccatgatgg atacagtggc gcaacgatac gttcaatgtt cttgacttgc cacagttcat
     1741 gcatgtagct gaagtcaaac accacaccga gttgttcttt gacgagctcc tcgttggcga
     1801 tgttgtcgag gccgagcacg tgaatcgacc cggcgtcgcg gtggatcatg ttgaggatga
     1861 gtttgatgag ggtggatttg ccggcgccgt tcgggccgat cagtcccatg atgtagccct
     1921 tgggcaggtc gaaggtcacg tcgtccagcg tgaagccgga atcatagtgt ttggtgacgt
     1981 cggtgacctg caacgccatc gcgtcggatt cggatatggc ggtcatggcc atggtccttt
     2041 cttgaggtta tttcttgcgg tattcctctt cgagcatgcg gtccaatgcg tccagcggaa
     2101 tgccggcggc tttggcgccc cggcttgcct cggccaggct ttcgcgaatc tgggtttcga
     2161 gctgacgttg catcagttcg ttgcccttgt ccatcacgaa agtgcctttg ccttggatgt
     2221 tctgcaccac gccctcatcg gccagttcgt tgtacgccct ggtgacggtg agtacggaga
     2281 tgcgcagctc cttggcgagt ttcctcaagg aaggcagggc ctcgcccgcc ttcaactcgc
     2341 cgctgagcac cgccgaatgg atttgggttt tgatttgctc gtagatgggt tcaccggaca
     2401 cggatgagat gatcagtttc acctatgccc ctttcgattg tggttgacta gcaccccagc
     2461 tgtttagctg ttatacaaca cagtataact gttctgctta actgtcaagg tgtgttacat
     2521 aacagttgaa cagtgaacaa acacctcgac aacatacagc gtcgaaacat gggccgtgcc
     2581 tacaatggcc tcggaaaggg gcacctatgc caaacaacgc cgttacgccg acagccccgc
     2641 cagcgggggt gtaacttttt tatcacagca cgcgcggagc gtcgtggaag tcgaagagga
     2701 cgccgtcggc taggtggcag atctcgggcc aatcggaggc ggaggagtcg tcgtgcatgg
     2761 cccagacctt catgccgacc gatttggccg acttcatgcc gaccagcagg tcttcgaaca
     2821 ccgtgcagtc gaccggcgcg gcgcctaggc gcttggcggc gagcagatag acgtcgggct
     2881 ggtccttgcc gacgtcgttc gcgtcatcga cggagacgat atcgttgaac agatcgaaca
     2941 tgccgacgtg gcgtagcgcc ggctcacgca gatgcggggg cagcgaggtg gcgacaccca
     3001 gtttgacacc ggaggctttc agatcgcgca gatagtcaag cgcgccgggt ttggcttcga
     3061 ccgtggtgga attggcgacg ctggccatgt cgtcccattc ctgcatgagc tcttcgggcg
     3121 tgtctgtaag gccgaagcgt gcgatggtgt attcggcgat ctggcggaat tgcatggcgg
     3181 agactttggt catgtagtcg ggcggcactt cgattccgcg cttgttcagg aagtcgatgt
     3241 cgacttggtc ccagactccc atggagtcta ggagggtgcc gtcgaggtcg aatatggcgg
     3301 ctttggtcat aggacacttt ccttcaacag ttctcgggcg tggtctaggg aggcttcgga
     3361 ttcgctgccg gagagcatgc gggcgatttc gtggacgcgg gtttcgccgt tgacttcggc
     3421 gatggtggtg accacgccca aatcgtcgtc atcggcatcc ggcggctcct tggtgaccac
     3481 gaactgcgcg tccgcccacg aggcgacctg cgccaagtgg gtgacgacga tgacctgcgc
     3541 gctgcgggcc agccgtgcca gacgtttgcc cagttcgacc gcggccttgc cacccacgcc
     3601 ggcgtcaacc tcgtcgaaga tgaaggtctg gtcggcaccg nnnnnnnnnn cggcaaccag
     3661 ttccaaggcc agcatcagtc ggctcagttc accgcccgaa gcgcttttgc ccatcggcaa
     3721 ctgcggcgac ccctcgaacg gcgtaaacag gaacgcgata tcgtcgcgac catacgggcc
     3781 gagcggatca tcggccttgt ctcgcggcgt gacgcgcact tccaaggagg cgcccagcat
     3841 ggcgagcgag ccgagttctt cagtgacgcg cgcgcccaag tcggcagcgg cagccacgcg
     3901 cgcggcgctc agcacgtcgg cggcggcgag ggc
//
LOCUS       DAWBMW010000148         3917 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_4826_length_3917_cov_2.994045, whole genome shotgun sequence.
ACCESSION   DAWBMW010000148 DAWBMW010000000
VERSION     DAWBMW010000148.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 3917)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3917)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3917
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4826_length_3917_cov_2.994045"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    714..723
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          830 a         1216 c         1168 g          693 t
ORIGIN      
        1 cgaaaagggc gtccaatccg atggcggcga ggccgtcgca gctccgggcc agacctccgg
       61 cgacccggtg aaggtggccc gcgattccat cgaactcgcc aagcagaagc tctacgacac
      121 ggtgatcatc gataccgccg gtcgtctggg cgtggacgag gagctgatga agcaggctcg
      181 cgacattcgc gatgccgtgc gtcccaacga aatcctgttc gtcatcgacg cgatgatcgg
      241 ccaggacgcc gtcaagaccg ccaaggcctt tgacgaaggc gtggacttca ccggcgtggt
      301 gctctccaag ctcgatggcg acgcccgcgg tggtgccgcg ctgtccgtgg cgtccgtgac
      361 cggcaagccg atcctgttcg cctccaacgg tgagggcctg aaggacttcg aggtcttcca
      421 cccggaccgc atggcctcgc gtatcctcga catgggcgac atcatgacgc tcatcgagca
      481 ggcgcagaag cagttcgatg aggaagaggc ccgcaaggcc gccgagaaaa tctccgaagg
      541 ttccttcggc ctggacgact tcctcgatca gctgcagcag gtgcgcaagc tgggctcaat
      601 gaagtcgctg ctcggcatga ttccgggcat ggcccagcac cgcaaggaac tcgagcagtt
      661 cgatgagagg gaaatcgacc gcaccgaggc gattattcgc tcgatgaccc cggnnnnnnn
      721 nnnatgatga agcgcatgtc caatcgtggc ggcatgggcg gcggcatccc cggcttcggc
      781 ggcccggcta tgggcggcgg caagaagaag ggcaagaaca agaagaaggg cggcaagtcc
      841 ggcaatccga tgaagcgcga ggccgaggag aaggcattgc gcgataagct ggccggcaag
      901 tcctccggtt cgaccggcgg ttccgccttc gccaagaagc cacagaaccc ggccctgccc
      961 gctggccttg agcagatgat gggcaacgtc gacggtggca ctgaactgcc gccgaacctc
     1021 ggcggaggcc ttggcggact gttcggtcgc tgatctcaca acttggctcc cctcgcagag
     1081 gggagccaaa ttatatctcg gttcaattta tatttcgaaa ggaacttcca tgacgacaat
     1141 cctcggcgcg gtggcaatca tcgcactgtt atggacgacc ttaagcgcgc tgcccgccgg
     1201 tctcgaggcc cgtatgccgc ttccgtacat gattgccctg acgccgctgt tgtgggttcc
     1261 tttgatgatt ctggccgcgg tcgccgcatg gcaacatgaa tggactgcga tgtcgttgct
     1321 tattgtggtc gcgctgattg ccagctcaca gcgcatcaac tactggggta cgtcaatcga
     1381 cccggccggg aaacgcaaag aatcccaccg tgaaacagcc cgtgaaacaa cttcgccgac
     1441 gctgtcaaaa agccataaca gcaatacatc gggtacctca aacactccga aggaagggcc
     1501 cgaaaccatc tccgatggac actgtgaaac aacgcgtgaa acactctcgc cccagttctc
     1561 ggttatgacg atgaattgtc gatacggacg cgccgacgcc gctgaaatca ttcgcaatat
     1621 tcgcgaacgc aatatcagcg tattggcctt gcaggaagtc accgacgacc tcatcacacg
     1681 tcttaccgaa gccgacatca acgaacttct cccctaccat cagttcggag acgccaaaga
     1741 gaccgataat ggcggattca acgtgatcta ttcccgctac gagccgagcg cagcggtgcc
     1801 caacgtggtc tccatccccg ccgccgacgt tcccgccatc accctcaaaa cctccggtaa
     1861 tcgcacggtg acgctttgtt cggctcaccc gaaatcgccg atgcgcggct gcgcggattg
     1921 gtcggccggc attctgggtc tgcgagaact cgcccgtgca aaatccgtca gcaatcgcaa
     1981 tatcgtcgtc gttatgggcg atctcaactc gagcaccgac catccgagct tccgcgcgtt
     2041 gctcaagtcc ggattcaagg acgcaagtct gattcaagcc gccggcccga atctcacctt
     2101 cccgagctgg ctcaagtggc cgcgcatcga gctggatcac gtgctgttca ctcccgggct
     2161 caaaccgtcc ggcgtgaagt cgtttgaggt cgagggcact gatcatctgg ccctgaccgc
     2221 aacgcttgca ctgcgatagc cggcattttc gcgatacatt ccccggaatg ttgcgcaacg
     2281 gggaacgcgc accacaccgc aaccacagtg cgccacgccc agtccggccc tgtgcgctat
     2341 aataggccag ttattcgcgc gcgcgtggcg ccctctacac cccgagccgc gaggacacgt
     2401 ggattccgga cggccatgcc ccacatggca aaccgagaac ccgcacacct agcattacaa
     2461 ggagagccat tttggcaacc aagattcgtc tgaagcgaca gggcaagaag ttctacgcct
     2521 tctaccgtgt ggtggtcgtc gattcccgca agaagcgtga cggcaaggtc atcgaggaga
     2581 tcggtaccta caaccccaac acccagccct cgaccatcca gatcaagtct gatcgcgctc
     2641 agtactggct cggcgtcggc gctcagccgt ccgagcccgt cttcaagctg ctgaacatca
     2701 ccggcgactg gcagaagtac aagggccttg aaggtgccga aggcaccctg aagaccgttg
     2761 aggctggccc ggacgccgag gcccgcatcg cggccgttga ggaccaggct cagaagctga
     2821 aggccgccaa gtccgaagct gctgccaagg ccaaggctga agccgaagcc gctgccgccg
     2881 ctgaggaagc cccggctgaa gaggccgccg aagaagctcc ggctgaggcc tgagcggaaa
     2941 cgaaaggcta gctatgcttg cacaagctgt ggaacacctc atcaagaaca tcgttgattt
     3001 tcccgacgat gtttccgtca agtctcatga gaacgcgcgc ggcgaactgc ttcgcgtacg
     3061 cgtgaacccg gaagacatcg gccgtgtgat cggccgtggc ggccgcaccg ccaacgcgat
     3121 tcgcaccgtg attcaggcac tgtctgacca caaggtccgc gttgacatca tggacgtccg
     3181 tcgataaccg ataaggaacg attcacagca tggcacagca agacatgcac gacgaccctc
     3241 agcagctcga gttgctgagg gtcgctcgta ttggccgcgc acagggcctg aagggcgaag
     3301 tcaccgtgcg cctgtacacc gatgatcccg agtggcgctt cgagccggac tccgtgctct
     3361 acagccagga tggcgagacc gagtacatcg tcgagggctc gcgcacgttc aaggatcgtt
     3421 ggattctcaa gcttgagggc gtcgacgacc gcaatgccgc cgaggcccta aacggtgtcg
     3481 aactctacgg cgaggtcgac gatgcggagg acatgctcga ggccgacgaa tggtatccca
     3541 aggatcttat tggccttgag gcccgtctag tcgagggcaa cggtctggga ctgcccgccg
     3601 gccaagtggt cggcaaagtg gtggatgtga tcgactcccc cgcccaatcc ctgctcaaga
     3661 ttcgtttgac cgagccggtg gtaaccggcc agaattccaa gggcgaggat gtggtcgaaa
     3721 agaccgcgtt ggtcccgttc gtggaggcct tggtccccga catcgatctg gaggagcaat
     3781 atctcacgct cgatccgccc ggcggcctga ttccgggctg cggcatgatc tgacacatag
     3841 ggggggcgct gcgcatgcag cgcccattac catgtttggt aggtcccgat ggtcggggcc
     3901 gggtcgccgg ttccggt
//
LOCUS       DAWBMW010000149         3875 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_4875_length_3875_cov_2.240576, whole genome shotgun sequence.
ACCESSION   DAWBMW010000149 DAWBMW010000000
VERSION     DAWBMW010000149.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 3875)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3875)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3875
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4875_length_3875_cov_2.240576"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    845..854
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3373..3462
                     /estimated_length=90
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          692 a         1042 c         1153 g          888 t
ORIGIN      
        1 cccatcttct ggatgtgctc ggggtccacg ccctcgttga tccacatgga acgggcttcg
       61 tcatcgtcgg tgaacgtggt catccacagc ttctcggggt cgaagccgta gccgccctca
      121 tcctgcgagg tggtgagcag ctcccaggcg tagtggatgg cctcttcctt gaagtagtcg
      181 ccgaaggaga agttgccgag catctggaag aaggtgccgt ggcgggtggt cttgccgacc
      241 tcgtcgatgt cgagcgtacg cacgcacttc tggttggagg ccatgcggcg cttcggcggg
      301 gtctgctcac ccatcaggta cgggatgaac ggcaccatgc cggcgatggt gaaaagcgtg
      361 gtggggttcg gggaaatcag ggatgccgag ggcacgatga gatggtcgtg cttggcaaaa
      421 taatcgaggt atcgctttgc gatgtccgca gtgcgcatga gcggatgaac tccttgtttg
      481 ggtaatgggc gcagttgcgc ctggtattgg tggaaaagac tggattatga ggattagttt
      541 gttctagctg attcagtgac tagatggcaa ggcggcatgc gcgtgtcagt tggcgcgctc
      601 gatgtactga cggttgagct cagcctcgcg ggctcgacgg gtggcattga actcgttgac
      661 caacccttcg agtgttttca tcggcacatt gtcctgatcg gggccaagca ggaactggcg
      721 tgccgcatca ggcgtattgg ccttgacata ggcctgtgcc ttggtgacgg caacgattcc
      781 gatggccacg ccgacgccga cccagaagat gcgtttgaac atcactcccc cttctcggcg
      841 gtgtnnnnnn nnnngcggtg tcgttgacgg gggttttcgc ggcgttcttc gcaaggaagg
      901 actgacccgt ggttttcagc gcgtagaagg tggaagcgat cttgatgatg ggcttaccga
      961 ggaaggagcc atacagatcg gtcaacgcac caacgttgtt ggcggtggtg gaagccgcgg
     1021 cggaaatctt gttcacgtct tcgagcgact tgttgacctg cttgacggtg gtcacgcctt
     1081 cgtccagcgc ggggatggcg tgttcaccgg actcccggac cgtctgcgat atctgatcaa
     1141 acatcttgcc cagcctgatc agcggataga tcatgaaacc ggccaatact gcgaacgcga
     1201 tggccgcgat caggccagcg atctctccga ctcccattgt ttcctccttg cggtcacact
     1261 tactcgccta agggtagcac tccttcccta tttcttggca taccattgca tgcgcagggc
     1321 gaaaaccgtg cgcgtgggcg tatgcggcta taggcgaaag gctgtcctgc tgacgctttc
     1381 gtttgcggcg tgccgccgtg gaccgtacgt gctattcgat atgtattgct tgctatttgc
     1441 tacttgccgt acgaaacgat ggtcacggct tcgccgaacg gcttgtcggt gtcgaagtcg
     1501 atcacggtta ccgaaccatt ggccgggcgt tcgttcagat cgaggtcagg gccgccgaac
     1561 cggtgaccga ggctaagcag tgtgttgcca tgcgaaatct gcagcacatc gtcgccgtcc
     1621 ttgagattcg ggttgtcggc gataagacgg aacgcacctt cgacgcgcgt ccaatattcc
     1681 tcatcggatt ccgcgtcgtg gaagggatcg gcttccttga gaaaatcacg cgaggccccc
     1741 aacccgaact tctcgacgat ttggttgtag gttttgacgc cgtggggcgc tcccgcggcg
     1801 taccaggcca tggccatgtc aagtccctcg tagtagccgt agaactgctc gcggaagtgc
     1861 atgtccgtca cgagtgcggg gcgcgggtta cccgcggctt cgttgatgtc gagaattttc
     1921 tcggccgtct gctgggcacg cgtggtgtcc gaacagtagg cggcggcgaa cgtcaatcct
     1981 ttcagtcgct caccggcctt gacggcatcg gcgacgcccg actcggtcag cggcgaattc
     2041 gaccatccct gcagacggtt gtatcggttg aaataggtct gtccgtgacg gaccagatgc
     2101 agatgaagaa tcatggttct gattatgtca ccattccggc ggtttcggtg cgggttgccg
     2161 gaagtcataa atcataacgg cataggtcac tacctgagat atgcgtaatc cgggcagtat
     2221 acctaacgat tcctgagagt acacttagaa tactgtgagc ataaccttga aacctgcacc
     2281 atctcgatcg tctgtgcctg cccatttcag gggtatcgat ggactgcgcg ctctggcgat
     2341 tcttggagtc atagcattcc acacccgccc gtcgctattg cagggcggat tcattggcgt
     2401 gacgatgttt ttcgttatca ccggttttct tgccacacgt tcggtactga acatggtgga
     2461 ccgcaccggg tcctttggat atggccgata tctgatccgc cgcattactc gtttatggcc
     2521 ggtgatgctg gcgaccattg ccgtggtgcc ttggctcact tggatgttcg ctcccagcct
     2581 gttgcagaaa gtggtttccg attcgctgcc gagcgccttg ttcgccagca attgggtgta
     2641 catcttccgc aaggtgccgt atttcgaggc ggccggattg ccttctcctc tgacccatct
     2701 gtggttcttg ggcgtgacga tgcagttcta tctggtttgg ccgttgctga tggtggtgct
     2761 cggcaaaatc accaagtcca aatgggtgcg ctccatggcg attcttgtca tcatggctgc
     2821 ttccaccgcc gacatggtgc tgctgtttga cccggccaat acctcccgcg tgtattacgg
     2881 taccgatact cgcctcgccg agctggccgc cggcgcgctg ttggctatct ggattgcgcc
     2941 ttcctcgcga ggtacggaag ctgcggaagc tggagcggct tcgcaaacag tgcagatcga
     3001 acgtcaggcc ggcgacaaga cgggcgcccg tctcactatc gtctgcaatg ttcttggcac
     3061 cgcggctttg gcggcactgc tgaccggatt ctggttcgcc aacgggtatc tgtcgtacat
     3121 gtaccaaggc gggtacctga ttaccgcgtt catatcgttg tgcgccttgg cctgtgccgt
     3181 gaacaatgac tcaatctggt cgcacgtgct gggttgtgct cccctacgct atatcgggtc
     3241 gcgatcgttc tcgctgtacg tcatgcatta tccgctgctg cagttcatga acccggcgac
     3301 acgaacgcag gcgttgccgt ggtggggttg ggttcttgag gccgtcgtgg tgttggcggc
     3361 gtcggaagcc ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnctcaagcg cgagccattc
     3481 agctgcgccc cgaactcgcg gcacccgcca agccggtcaa gaaaccggcg aagcccgagc
     3541 ccgagtcttc tgccataaat gccgcgaacg gcgaaaccaa taccgctgac gcgaataaga
     3601 acggttcgga ttctcagccg cccatcatca aaccgattgc ggaaaaggtg ccaaacaatc
     3661 ttgacatttc gaagtacgcc tatgatccgg caacggaaag ctgcaacgcg gatatgatga
     3721 tggtcggcga ttcagtgacg tcgggtactt cggacgccat ccaggccgca ttcccgaacg
     3781 cttttatcga tggcctgccg aaccgtcagc tgccgcaggc ggtggatgtg tatcagcagg
     3841 tcaccgctgc cggccacagt ggcagtgtgg tggtg
//
LOCUS       DAWBMW010000150         3850 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_4930_length_3850_cov_2.122530, whole genome shotgun sequence.
ACCESSION   DAWBMW010000150 DAWBMW010000000
VERSION     DAWBMW010000150.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 3850)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3850)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3850
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4930_length_3850_cov_2.122530"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1404..1413
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3396..3405
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          821 a         1339 c         1070 g          600 t
ORIGIN      
        1 tcagcgtacc gtacgggaga ttggggataa attcggacgg atagatattt tggtaaataa
       61 tgcgggaatc tttgatttca ggttcatcga tgagatggat tcgctgctac gcacccgcgg
      121 ctccggcgtc tcctccgatg tggagaccac gattgtgccc cagttcctct ccgaactcga
      181 cggcgtggaa tcactcgaca atgtgatggt aatcggtgct tccaaccgcg tggacatgat
      241 cgatccggct gtgttgcgac cggggcgtct cgacgtcaag attcgcgtcg gccggcccaa
      301 aaccaatcag gccatcgcca tcgtggacca ttacctcacc gacgacctgc ctctggaaga
      361 tggcgtcgac gcgcacgcct tgtccgccgt gctggtgcat gacatctacg gaacctccga
      421 acgccgccat ctatgcgacg tgcaggagga gaacggccag tggcatgcgt tgttcctggc
      481 cgacgtggtc tccggcgcga tgctcaagaa catcgtggac cgggccaaga cccgagcggt
      541 caaggaatcc atcgaaaccg gcttggacgt ggcactcacc gtgccgctgc tcgctgccgc
      601 cgtggaagac gaataccgcg agacccgcga ttccatggcc gacgtggacc ccgaacaatg
      661 gtcgcgcatc aacggcatgg acccgatccg ccgcatccgc accgccgaat agctcaagct
      721 caaggagcac tacatgaccg ttaaacgagt gatgggcacc gaaaccgaat acgcggtatc
      781 cctgaacacc ccggaccgct acaatccggt gcaactgtcc ttcgacgtgg tgaacggcgc
      841 cgcggacagc cacagcaaat ccatccgctg ggactaccgg caggaggacc cggtcaacga
      901 cgcgcgcggc acccgtctcg aacgcgccgc cgcccgcccg gacatgctca ccgacgcccc
      961 gcagctcaac atcaccaacg tgatcgcccc caacggcggc cgcgtctatg tggaccacgc
     1021 ccaccccgaa tactcggcgc cggaaaccac cgacccgttc gaagcggtcc gatacgacca
     1081 cgccggagac ctcatcatgc aggccgccac cgaacacgcc cgcaaacaga ctggcactcc
     1141 cattgccctg catcgcaaca atgtggatgg caagggctcc tgctggggca cgcacgagaa
     1201 ctatatgatg gcccgcgcgg tgccattcga cttggtcaca cgcctgatga ccctccactt
     1261 cgtcacccgc cagatctacg ccggctccgg ccgcgtaggg attggcgaga acagcgagat
     1321 tccgggctac cagctcagcc agcgcgccga ctacatccac gccaaagtcg gcctgcaaac
     1381 caccttcgaa cgcccgatca tcannnnnnn nnnccaccga tgcataccgc cgtctgcacg
     1441 tcatcgtcgg cgacgccaat cgcatggagg tgccgcaggc actgaaactc ggcaccacga
     1501 gcatgctgct gtggctgctc gaacatgcgg acgaggccgg attcgacctg aacgccttcc
     1561 tcgacgaact cgaactgtct gacccagtcg aggccataca caccgtttcc cgcgacctga
     1621 cgctgggggc aatcttgccg ctggccaacg gcggcgaaac caacgcatgg cttatccagc
     1681 tcaaactgcg ccaagccgtc taccaggttg ccgcactggt cgaaggtacc gacaccgccg
     1741 gcgaacccgc ctggccggac aaatccacca catccattat ggcgatgtgg cagcaggcgc
     1801 tcatcgactg cgcctccatc cgccatgccg ccgacgatga cgaacgtctg gccatgaccg
     1861 gcgaggccag ccgcatcgaa tggctgctca aatggcagct gctcgaaaaa ctgcgccgca
     1921 aaatcaccac aacgtccgca cccggcgtgg ccaacggatg gaatgatccg cgactgaagg
     1981 tcatcgacct gaaatgggcg gcgctcgacc ccgcggacag catcttcacc aaactcgaac
     2041 cccgcaccga acgggtggtg agcgccgccg acatcgcacg cgccaccacc gaaccgccgg
     2101 aagacacccg cgcctggctc agggcaaaac tcgtggcgca attcggcgat gaggtggccg
     2161 ccgccagctg gtcccgactc actgttcgcg acccgcacgc cgcagacgag ggcgaggctg
     2221 tcgaattcta cggcaatgcc ggccatatgg ccgctaccga ccatcacctg ttctccctcg
     2281 acatctccga tccgctcgcc ttcaccaagg cgcactgcga aaccgaactc aattcagccg
     2341 aacacgccat cgatctgctg aagtcgctgg ccatcaacgc cgaacgatag acttggtagc
     2401 tatggaagaa acacgcgaag agaccccaca ggacctgcgc aaccttgcgc tcaaagtcgc
     2461 cggcctgctc gaagaagccg gccagcatgc cctccacgac caactcaatc cgcgtaacct
     2521 cgcccagccg agcaccgaga acgcggaccg tggcccccgc tacaagcttg aagtggacaa
     2581 caagatcatg cgcttcatga gcgccagaat cgccgacatc gcccacttcg acggcttctg
     2641 gaccacccgc cccgccgaaa gccgccctgg ccagcgttac tggtacatcg gcaagatcga
     2701 cggtgtgatc aactacgtac gtcgcatgag cgaatggacg gtgaccgccg ccctgttcga
     2761 attcggcccg gacaacgagc ccaagccgat catcggcatc gtgcacgccc cggccctcgg
     2821 cctgacctac ctggccgcca ggggagcggg tgccgtgcgc atccacaaaa ccgcggtcgg
     2881 cgagaagcgc gacaaggtcg tgccgtcgat gacctcctcg ctggatggtt ctgtgctgag
     2941 ctacggcatg tccttcatcc ccagcgaatc gcagcgtgcg ctcgacgtgg cctcctcgct
     3001 cgccggacgc ccggccgaca tcaagcgcgt gggccccgtc tcgctcgacc tgtgcaaagt
     3061 ggccgacggc acctatgacg cctacttcga gccgatgctg cacgtgtggg atatcgcggg
     3121 catcgccgcc ggcaccgtag tggtctggga ggcacagggc acgctgagcc gttgggacgg
     3181 cgaacgcatc cactggcgtc acgataacga tgtagtggcc tccaatggac tcatcatccg
     3241 cgaactgcag cattacctgc agcagtatcc gtggctcgac tccatcaacc agcgataatt
     3301 ttttgactcc tccggatccc ctcgggtgag gggagccgtc ggccgaaggc tgactgaggg
     3361 gagtttgaca gcccaaaaag aagacaacat gccacnnnnn nnnnnctcaa gcacagcagg
     3421 ccgcgaccca ggaagacgac gccctcgcca ccacgcaggc cgccgcccaa accgagtcca
     3481 ccgaccaagc cgacgtgttg gacgacatcc tcgacgacat cgaatccgcc ttggaaacca
     3541 acgccgagga atacgtcaac agcttcgtgc aaaaaggcgg cgagtgatgc cccaactgcg
     3601 tgacagcggc acccgctccc tgcacgccac ggaacccgta ccatccgccg agatggacgg
     3661 gttctgccgt attttcggcg tcgaaaccga atacggcgtg gcggtcaccg gtgccgaacg
     3721 ccccgtggac gccggccagg tggcgatgac catgttccag cccatcgtct cccgttcccg
     3781 ctccaccaac acgtatctgg ccaatggctc acgtctgtat ctggacgtcg gatcccaccc
     3841 cgaatacgcc
//
LOCUS       DAWBMW010000151         3837 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_4953_length_3837_cov_2.080381, whole genome shotgun sequence.
ACCESSION   DAWBMW010000151 DAWBMW010000000
VERSION     DAWBMW010000151.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3837)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4953_length_3837_cov_2.080381"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          699 a         1118 c         1211 g          809 t
ORIGIN      
        1 ttcacggtgg ccttctggcc ctcgtgctcg ccggcttcca gcgtgccttc gaaggtggtc
       61 tcctcgccgg cggacaggcc gtcaagggcc tcgtccagac cgtcgagcat ggtgttggag
      121 ccgagctcgt agctcacgcc ttcctgggag tcgacggtct cgccgtcaat ctcagcagtc
      181 aggtcgatgt tggcgaagtc gcccttggcg gccgggcggt ccacgccgac caaggtgccg
      241 aagcgctgac gcagggtctc gaggcgcttg tcgacgtcct catccttgac ttccggcttg
      301 gagatggcga tctcaagacc gtcgatttcc ggcagttcga tgtcaggacg acgctcgacg
      361 gtggcggtga acttcagctt ggtctcgtcg ttggcggact gcggaacatc ctgcacgtcg
      421 aactccggct gagccatcgg acgaatcttc ttctcatcca gggccttgga gtacagctcc
      481 gggaccgcgt cgttcacggc ctcaccggcg acggcggcga aaccgatgcg ctggtcgatg
      541 atcttgccgg gaacgtggcc cttacggaag ccgggaacgt tcacctgctt ggcgatttcc
      601 ttacgggccg catccagata cgggttgagc tcttccggtt caacagtgac ggtgagcttc
      661 accttggtgg gctcgaggtt acgaacgctg atcttcacgc taatgactcc tcaaaagtcg
      721 ttatttctta cagttctgcc gtaaggcaac ctctacatga taacgccact tacggacggt
      781 gcaatgagtt tcacttgcca gttccttgcg ccctgctcga tcaggaacgt ctcgacgtcc
      841 acttccaccg ggtcttccac ccagcacagg ttgcgtagga tctgtggctt gatgatcatc
      901 tcggccggcg tgcgggtgtc ttcggcgatg cggttgatca cacgccgcac ccgttgcaga
      961 cggtcataac gctccggatg gcgctgttgc cacagccgca tggatcgggg cgcgttcacc
     1021 acaccgtttt cgtcctgttc gggggcgggc atggtaggcc attgggtggg cttcagggct
     1081 atggctcgat cgatggcctg cttccagacc ttcggtttga ccgcacgctg aatcggcgcg
     1141 taccgctcga acattttgtc ctgctcggtg ccggtatgga tgcgcacacg ctcgttcagg
     1201 gatcggagcg cgcggaattg ggcagcattg tgcggcttgt tggtcgccgc ctcgatgatt
     1261 gatgaatcgg ccaataacag gctgggcgca atatcgtatt gccgggcgag tcggtcgcgc
     1321 tcctcccata gggatttggc gatggccaag ccccttggat cacgactcag ctgggtgatg
     1381 cgcgagatac gcagccaagg aatcggatgc ggtttgcgcg gggcaaggcc cgcaaccaaa
     1441 gcgtgagaaa actcttcagc ggcccactcg tccttgcctg cggctttcag gtcacgacgc
     1501 atcatggttt caagttcgat aagcacctcc acgtcgagcg ccgcatagtt gcgccagtcg
     1561 cgcggcagcg gacggtagga ccaatcggcg gcggaatgct ctttggctag cgtgatgccg
     1621 aggtagtgtt cggtgacggc ggccagacca aagcggtgca ggccgagcaa tcgcgccgct
     1681 atttccgtgt cgaacagggc tttgggtttg agcccgattt cggcgaatcc gggcaagtcc
     1741 atcagagaat catgcagaat ccacgtggcg tcgcccacgg cgtcattgaa ttcattccaa
     1801 tcagctccgg cccgggtcag ggcgacggga tcaagcaagg cgatgcctgc accctcacgt
     1861 ttgaactgaa tcagccaatc ctcgtgtcca tagcggaagc cggacgcccg ttcggcgtcg
     1921 gcggccagcg agcccgtcgc ctcggccatc gcatcgcaga cacgatgata gccgtccagc
     1981 gtcgaagtga catcgggtac tcccccgcga ggctctttca gcagtcgggg ttcggtgtca
     2041 cggacgtcac tcaactggcg tcatcctcct caaaacggat ggtggatctg atgaatgcgg
     2101 cccacgagcg gacttgtgcg ccggcgtcca tagtaccgtc cggcccggtc ccgtcaagcg
     2161 gggtccatga gacgcgaatc tcacacccgg cgctggtgga gccggccatc gagccaaaag
     2221 ccgtgttttg ggtcacggtg actgtgccgg ataccgaatc cggctcgatg ccgtcgaaat
     2281 agtcgcacat gtcatcccag tacatgccgg gggtcagaga atcgttttcg gcggtttcca
     2341 gcggcagtcg cgcgaacgcc acgcaacgcc agttggaatc ccagtcaatg cgcagctcat
     2401 gagcataaag catcatgatc cagcctgtgg cgattcgtga ttcggcatcg ctgccaagac
     2461 cggcggaaaa ggcatcgccg agggcacgct ctcccgcttc gaattccacg ccgataccat
     2521 aatcggccaa ggtggaaggc acgggaatct cccgatagcg cacgtcatgg atgcgcttca
     2581 tggtgctgac ggattcgacg gctgcccata cttcatccgg cacaccctga gggcgcacgg
     2641 ggcgtccgtc gttggactct cccgtaggaa atgcgtagat gtcggccata tctctagggt
     2701 atgggcgaac gggccgaatc gtggtattta cgcgcggttc ggccctgcaa tttaatacac
     2761 ggtgaagccg tggtccttga actggttggt cacccgttcg cgggtggcgg cgttgggtcc
     2821 cttctggttc tccagcgggt atgggatgcg cagttcgtgc cacttcgggc ggccaagctg
     2881 gtggaatccc agcacgtcga tgtgttcgac ggcatcaccg aaggactcgc agatcttcgc
     2941 cacgttttcg acgttctctt cggagtcggt caggcccggc acgagcacaa atcgcaccca
     3001 gatcttcttg ccggccttgg ccagtcgctg gccaaaatcg atggtgggct gcaaggtgcc
     3061 gccggtgacc ttgtgatagg tctcctcgtc gccggatttg acgtcgagca gacacaggtc
     3121 gatgtcctcg agcatctcgt cggtgtaatt cgtgttgagg aagccggacg tatcgaggca
     3181 ggtgtgcacg cccatctcct tggcggcatg gaacacacgc gagacgaagg cgggctgcat
     3241 catggattcg ccgccggaga aggtgatacc gccatgcgtg gccttgaaca ggtccttgta
     3301 acgatcgacc ttcttgatca tggcgtcaag gtagacgggc ttgccgtcgc gcatcttcca
     3361 agtgtccggg ttctggcagt actggcagcg cagcgggcag ccggacatga acacggtcat
     3421 gcgggtgccc ggtccatcca cggaggtgtt gatgtcccaa gagtggacga agccgatatc
     3481 accggatttc agggcggaaa ggcggtcacg tcggtcgagt ccgattgggg attcaaagcc
     3541 ggaaaggcca cccatcaaag tctgcgaggc ataggtcttc gattccttga gcatatgcct
     3601 ggtcgtggtg cggaacgtgg gtgttgcggg catcgttgtc ctcctgttcc aaatgatgcg
     3661 aaagtctgtg ggaaataagg aaggggtgga gccgtgcccc accccttcag ttagtcattg
     3721 ccgcggttcg cggggttggt catcagtcga cgacggcacc ctggtggaag gtacgggaga
     3781 tgacgtcgag ctgctgctcc ttggtgagct tgacgaagtt caccgcgtag ccggaga
//
LOCUS       DAWBMW010000152         3794 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_5014_length_3794_cov_1.944103, whole genome shotgun sequence.
ACCESSION   DAWBMW010000152 DAWBMW010000000
VERSION     DAWBMW010000152.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 3794)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3794)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3794
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5014_length_3794_cov_1.944103"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          730 a         1081 c         1211 g          772 t
ORIGIN      
        1 gtagagcacg ttgtcgagct tgtgcgattt gccgaaggtg cggcggcggc ccacgggaat
       61 ctcgctgtcc gtctggggct tggcggcacc catggcaagc ggtgtggcgg cgtgttcgcc
      121 gtccgtgacg ctgttggatg cgtccgagtt caattgcgtg gtggattcct cgccgcgcag
      181 ccaattcgtg tcgacgtcca gttcgctggc caggaattcg agcacgtcct cgcggggcat
      241 ggtcttgccg gccacatatt ggctgatgtg gctcttgccc atctttacgc cacgatgttc
      301 ggcggcgtgc accaaatcga cctgcttcaa tcccctgctg ttcatcgcat tgcgcagacg
      361 ttccgcaaac accgatacgc tcatatcctt atgcctccaa ccgggagttc aatttatctt
      421 ttgaacaacg gttcaaagca tacccccggt tcaacacaag ttcaattttg gcgtgttctc
      481 gagtctcgac cggttcaacc caactgcgct tcaatccatt gggcgacgcc gtcctcgttg
      541 ttggacgggc agatctcgtc ggcgacggac aacaccttgg ggttcgcgtt ggccacggcc
      601 acgccccggc cggattcgct catcatgttg atatcgatga ggtcgtcgcc gaaggcggct
      661 gtctgttcaa gcggcacgtc cagtcgctcg cacagcaggt gcagcgcgtg ctccttgttg
      721 gcctgcgggt tcatgagcat ccacatcacg ccttcgctga tggagatgtc gaagtccggc
      781 ggaatcagcg cacgcagctc gttccactgt tcaggttcgg ggaagatggt gattttgtcc
      841 gccacgcctt cgggcacgtc gtggaagttc gtgtattgga aggcctgagt tttccaatac
      901 acgcgcacat cgaagttggt gtagcgcacg tcgttcatca cgatgccgag cttcaggccg
      961 ggcatgcgat cgagcaggaa cagacagact tcgcaggcac gccgtgagga gaagccaatc
     1021 ttgatcaaat ggttctcatc ggccggcgtg gcacctgagg tgagcaggtc gaagtcggag
     1081 tgggccgggt cgaaatcgat gagggcgccg ttcagataga ttacggcgtc caccggcagt
     1141 ttttcggcga actggagacc ggtactcacc ggccgcgcgg tggcgatggc gaattgcatt
     1201 ccggcatcgt gcgcgcggtt gatggtgtcg atgctgcgct gggtaatgaa ccggtcctcg
     1261 aatacctcgg cgtcgtgcag caacgtgccg tcgaggtcgg cgaccagcag tcggggttgt
     1321 ttcggcgtga tggcgtcgag aatcacagca gcccctcaca gtgttcacgc acgagttcgg
     1381 cggaatggtg cagtttggcc cgctcgtcct cgctcagatc gagttcgact atctcgttgg
     1441 caccattggc gcgcagttca gtggggacgc cgaggaacac gtcatgttcg ccgtattcgc
     1501 cgtccagcag ggtggagacg ggcacgatgc ggcgttcgtc ccacaggatg gtctgtacga
     1561 tgccggccac ggtggaggcg atgccgaagt tggtgccgcc cttggccgcg acgatctcat
     1621 tgccacgggt gcgggtcttc tcttcgattt cggtggtgga cacggaggcg aaacggtctt
     1681 ggttgtcggc caggaagcgg gcgaatggct tgccgccgag cgagacggtg gaccatgcgg
     1741 tgaactggga gtcgccgtgc tcgcccatca cgaagccgcc cacgttgcgc gggtcaaggc
     1801 cggtctcttc gccgatgatg gtcttgagac gcgaggtgtc gagcgcggtg ccggtaccga
     1861 gcacctgggt gcgaggcaga ccggaacgtt tccaggcata ccaagccatc acgtcgaccg
     1921 ggttggagac catgacgatc acgccgtcaa aaccggaggc catgacgttg tcgaccactt
     1981 cgcccacaag acccacggtg aagccgagtt cggccatgcg gttggagttg gccggcggct
     2041 tgcggcccac ggtgatgacg acgatgtccg catccttgca gtcggcgtag tcgccggcgc
     2101 gcaccttgac gtggcggtcc tggaattcgc tgccatcatc gaggtcgcgg gcctccccca
     2161 gggccttggc ggcggaacag tcgatgagca caagctcatt gcacaggccg tgcgtgacga
     2221 tgccgaaggc ggcggtggca ccgacccggc cggtgccgac gataacgact ttgttgcggt
     2281 tcatagtgac catggtgatg tcctttcgta atactcgata gaacgcatac gacccatgac
     2341 cgacggtaac ggtcatgggt cgcgagcgac aagtctatat ggcgtgcaag ccgatcagcc
     2401 gaggttgaac ttggtcatca tgtcgaggcc gatgatcaag gccacccaca ccagagcgat
     2461 gataacggtc caacggttca ggttcttttc ggcgacaccg gaagtaccgg cgttctgggt
     2521 cagaccgccg ccgaacatgt cggacaaacc gccgcccttg cccttgtgca taaggatgag
     2581 cagggtcagc acgaagctga gcagcacgac gatgacttgc agcgtgatct taagagcggt
     2641 cacagggtac ctccggattg attggactgt tgaccagcat agcgcacaca cgggaaaacc
     2701 gtggtattag ttccgtgatt tcgtggattt cagtgccagt cgagcgattt tggcgagttc
     2761 ctcaacgtcc agggccgccc ctccgatcag gaagccgtca acgtcgggct gggaaatgag
     2821 ttccgccgca tttttcgagg tgaccgatcc gccgtacagg atacgtacgc tatcggatac
     2881 cttcgtgcca aaagtggtct tcaaatcgtt gcggatggcg tttgcggcat cctgggcgga
     2941 ttgcggagtg gccaccatgc cggtgccaat ggcccagaca ggttcgtagg ccacgatgag
     3001 tttggcggct tgttcttcgt tgagatcgcg ggtgacgtct cgtacctggc ccacggcgaa
     3061 atcgagttca atgccttgac gtcgttcctc gaagctttcg ccgacgcaca aaatcggctg
     3121 catgccggcg gcgagcacgg cacgtacctg atcgacgata ttggcggcgt tcggagtggc
     3181 cgacgatgac gtatgagcag ccaagatggg cgatcatgtc cgcggacaca tcgccggtga
     3241 aggcaccttg ggtggtcacg gaaacggatt gtgcgccgta ggcgacatgg agcttgtcgg
     3301 cttcgaccag cacctgcacg ctgcgcaatg acgtgaacga ggggaaaagc gcgacttcgc
     3361 agcgtttaaa gtcgaagtga gcgtcacgca acagccatac cagcttctgc acgaagtaag
     3421 tggcttcaag atggtcgaaa ttcatcttcc agttgccggc caccaacgga atgcgtttgg
     3481 acgccatatg tttcccttct aaaaaaaaac gtagctcccc tcaaggaggg gagctagaaa
     3541 atggattact caagcacctt caggccaggg agctccttgc cttcgaggaa ctcgagggaa
     3601 gcgccaccac cggtggagat gtgggagaag ccgtcctccg ggaagccgag gttgcgcacg
     3661 gcggaggcgg agtcgccgcc accgacgatg gtgaacgcac cggctgcggt ggcgtcgacc
     3721 agaccctggg ccacggcctt ggtgccggcg gcgaactcag gaacctcgaa cacgcccatc
     3781 gggccgttcc aaac
//
LOCUS       DAWBMW010000153         3755 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_5079_length_3755_cov_2.260270, whole genome shotgun sequence.
ACCESSION   DAWBMW010000153 DAWBMW010000000
VERSION     DAWBMW010000153.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3755)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5079_length_3755_cov_2.260270"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1570..1579
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2248..2257
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          868 a         1096 c         1077 g          694 t
ORIGIN      
        1 ttccgcttcc gctgaggtct ccggcaagcc ggttgagacc tccatgggtc tgaccccgct
       61 tgagggcctg atgatgggtg gccgtaccgg tgacatcgac ccggctgtgg tcttccacct
      121 gattcgcaac gcccacatga acgtggacga gcttgacgcc ctgttcaaca agcgttccgg
      181 catgatgggc atgaccggct atggcgattt gcgtgaggtt caccgtctgg tgtccgaggg
      241 caatgaggac gccaagctgg ctctggatgt ctacgtgcac cgcatcgtca gctacatcgg
      301 caactacacc taccagatgg gtggctgcga cgtgatcacc ttcaccgctg gcgtgggcga
      361 gaacgacgac gtggtccgca agatggtgtg cgacaagctc gctccgttcg gcgtcaagct
      421 ggacgaggag aagaacgcca cccgctccaa ggaaccgcgc atcatctcca ctccggactc
      481 ctccgtgatc atcgccgtga tcccgacgaa cgaagagctg gccatcgccc gcaagtccgc
      541 cgcaatcgcc gaagccggca ccgacaccta cggcaacacc ttcgccaagt aagtaaccgc
      601 cgttaggcat ctcaccaatc tctagttgca acactatggg ggctgattcc gattggaatc
      661 agcccccatt cgtatatggt catgagacca ctacccccgc gtcatactcc cctcagtcgc
      721 ctccggtgga cagatccact cagagagggg atccaggccc tataacttgg ctcccttctc
      781 tgaggggagc tgtccgcgta gcggactgag gggagttcca ccagaacaca ggacccctta
      841 ctgcctcagc atgccactcc acataccgac gaaatcaggc aaggtcttgc gcgtggtggc
      901 cacgttgatg acctcgatat cgggaatacg cagccccagc atcgcggcga acgtggccat
      961 acggtgatca gcataggttt ccatcacggc accgtgcaac gtctccgccg gcaccggttc
     1021 gatacgcaat ccatcaggca attcctcagc cgctccccca acacgacgaa tctcgttgac
     1081 caatgcctcc agacgattcg tttcgtggcc gcgcaggtga ccaataccaa ccatgtctgt
     1141 cgatttatca gcaaacacca gaatcgccgc caacgagggc gcaatctccc cggccgccgt
     1201 caaatcgaac gtacccagcc cacgcacggt gccgtcaccg gtcacctcgc aatagcgcac
     1261 gccgccgatg gtcgggaacg atacttcggc acccatctgc tccagatatc cgggcaacag
     1321 gccgcccggc tgggtggtgg tttcaggcca atgaggcacc cggaccgtgc cgccggcgat
     1381 caatgccgcg ccgaggaatg gcgccgcgtt cgacagatcg ggctcaacgg tcaccttgct
     1441 cgacagttgc attgcgcgcg gttcaaccgt ccatgtgcgg gcggattcgt cggcctcaac
     1501 cgcaccgccg gcaccggtca catcggccac ggtcatgcga atatgcggca ggctcggggt
     1561 cttctccccn nnnnnnnnnc gcgaggtgca gaccgccagg cagtttggaa ctaatcagca
     1621 acaggccgga gatgaactgg gaagaaccgg acgaatcgat gctgacctgc gcctgggcag
     1681 ccggcaaggt tgcaggcggc gtaatagtga acggcagacg gccaacctcg ccgtggtaat
     1741 caaccgttgc acccagctgt tccaaaccgt caagcaccgg cttcatcgga cgtgcataag
     1801 cctgttcgtc gccatcaaaa ttcactgggc catctgcaaa cagggcaagg ccgggcacga
     1861 aacgcatcac cgtgccggcc agtccgcaga acacattgac gttgccatgg aatcggccgg
     1921 atacaggagg agtaaccgtt acggtggtgt cggttgcgga atccacgtcg cagcggacac
     1981 cgagggcttc gagtgcgccc atcataaggt ccgtgtcgcg cgaacgcagc aggccaatca
     2041 gagtaacggg cttgctgccg agggcggcga gaatcaggta gcggttggat agggacttgc
     2101 tgcccggcac cgttaccgta gcgttcaacg gctgtgcggc gagcggggca ggccagagat
     2161 tcgtgagtcg ttcagtcata aggccaaatc tatcaaacaa attattcacc ggtcggcagc
     2221 gatgcgggat tcgatctgct gatcaggnnn nnnnnnnatc ggcctcgatg gcggcgatct
     2281 ggcgcgagaa cacgatgaac cagccgagga acgccagacc agccaacgcc tccacattgg
     2341 tgagggtgtt gtgacccatc atcatattga cccagaatgc agcgcatacg agcgcggtca
     2401 ggtcggaggc caccatcact gctttggaca ggcgtggcgc aagccacggc aggagcacga
     2461 acagcagcag catgacgaac ggcaggcctt tggcgcatat attgtgcaag agattgtgtg
     2521 gcgtgtaccg aaacacgccg atgccgatga atgccaggcc ggccaccatc agcaggaagg
     2581 acagaatgga gatgcgcagc atgaaatgtg ggatttcgta gccgcgattc ggatctctgt
     2641 tggtgcggtc gtgccagagg cgctgtagcc gttcggtggt caccagttcg gagatggcga
     2701 agtagctgac gatgatgatg catgtgccgg caagaatcag cgtggagttg aacatacggg
     2761 ccgcaaacgt ggttctgtcg ccgagctgcg aaaagttgtt gtgataccag tatgggtcgt
     2821 cggtggtcat gccggcggtg accacgccgg agatgacgaa gaacggcaac agactggcaa
     2881 tagttttggc gttcatcatc tccgcctgta caaaggtcat gtaccccacc acgccggcga
     2941 aggcggcgca gacgctggag atatagccgg agaagatggc attgcccatc atgtcgttgg
     3001 ccgcaccgat gagtgcaaaa gacgacagga aaatggtcga ggcatacacc accgaaaggg
     3061 cgacgatttc gaaaatgcga cgaatcggtg caaaccaacc atgtttgagg ttcaatgaac
     3121 gtgatttgcg cgcatagcca ataatgaagg aaatgacgcc gcatgccgcc acgatgcccg
     3181 aacataccat gaatcggcgt tgcgtcagct gccagatggc cggcgcgtat tccatgtaca
     3241 ggttcatcga taccgcgcca acgacggcgc aaatcaagaa ggagatgagt ccggaggttt
     3301 ctgcacgctg atgacgtccc atgccgggcc tcccctatct attcgatgcc gcgttatctt
     3361 tcaacaacaa acaaaactcc ccccgaggac aacgctttac tagcaagcca gcgggatttg
     3421 gctgtcgcca ttctactgaa aatctgaaac actacaaaaa aacgattatg tacgggcttc
     3481 ttagcctttg cgactatcgt taccgccttg ccgagtaccg ttgttcatgc agaaagaaag
     3541 cagtattgga cacaatcgaa tgagcgtgtc agtatcgttg aaaaagtaat gaatgacggt
     3601 tctatcggtt caacctttaa gggacgaacc agccgcggta ccatggcgct gatgcatgac
     3661 tatgccgagc tcatcaatga attacgtccg acagcagagc aattcaccga gatcgagcaa
     3721 tactgggtca aacgaattca ggcgttcttc accag
//
LOCUS       DAWBMW010000154         3744 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_5100_length_3744_cov_3.021957, whole genome shotgun sequence.
ACCESSION   DAWBMW010000154 DAWBMW010000000
VERSION     DAWBMW010000154.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3744)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3744
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5100_length_3744_cov_3.021957"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          710 a         1081 c         1177 g          776 t
ORIGIN      
        1 gataggcgtg ctcgaagaac gcggaattgt agcggccggg agtcagtacc acggcgatgc
       61 cctcggtgga gacaaagtcc atggctttgc gcaacaggcg cgggtattcg cgattatcgc
      121 gaatacgaac gtcgcggaac aggcgcgggg agatgcggcg ctcgatgtcg cgcacgaaca
      181 gcgggtagct ggcacccgag ggaatacgca ggttgtcttc cagcacccac catcgtccgt
      241 cctcgccttg gaccaggtcc tcgccggcga tgtgagcaaa aatgccgccg ggaggggtca
      301 cgccgttgac ctgcgggaag tagccggccg aggtgtatac gtattcttcc ggcacgacgc
      361 catcatgaat aatgcgctta tcggaataaa tgtccttcag ataggcgttc agggcgttga
      421 cgcgctgctt caggcccttc tccaattcgt cgtattcgtc ggattcgata atgcgcggca
      481 cagggtcgta cgggaacagc cggtcatggt attcgccgtt cttgtagatg ccgaaacgca
      541 caccgttgcg ggacagctct cgattgatcg cttcgcgacg gtttaccagt tcatgggcca
      601 gtctgcgtgc tgcggattcg atcatcgtgc ctgctccttg ctgtggcggc ttctgtggct
      661 tcaagcggtt aggcgttttg agcgcacgct gacgccgcaa gccgtaaccg ggtttgatgg
      721 tttcaaccta aggcgggccc gtttcaacaa acgcgcgaga atgttacagg caggtcactt
      781 cgccgaccgc acgcatcttg ccgaccgcac tcatctcgcc gaccacactc gttacaatga
      841 atggctatga cacgactgcg cattgacttg gcctacgacg gcggcggatt ctatggctgg
      901 gccaaacagc ccaatcttcg tacggtgcaa ggcaccattg aagatgcgct gcacaaggtg
      961 ctgcgggtgc cggccgatga cgcggccgaa ccgttacgct tgacagtggc tggacgcacc
     1021 gacaccggtg tacatgcctc ccaccaagtg gcgcatctgg atgtatccga tgaggtactg
     1081 aatcgctgcg taggtcacat gaccattccg gtcaccgagg cgctgactcg cagactcaag
     1141 gctgtgctgc ctccggacat cgtcattcat ggcattgccg tagctccggt cggcttcgat
     1201 gccaggttct ccgcattgga gcgcacttat gtgtatcggg tcgccgatcg ttcgtctgag
     1261 gtcgacccgc gcctgcgtgg atgcgtactt accgtggatg aagcgctgga tctggagctt
     1321 atgaaccgcg cagcctcatt gaccatcggc ctgcacgatt ttggttcctt cgccactccg
     1381 aatccgggag gcactacgat ccgtgaagtc aagaccgcct attggcggcg ggtgccgatc
     1441 actcccctag taccggatga gatggcatcg catgaagcat atcggactcc gtcgctggag
     1501 tccggcctgg tagtgttcac gattgtggcg gacgcctttg cccgcaatat ggtgcgctcc
     1561 ctggtcggct catgcatcaa agttggttcg ggacgcaaat cactggagtg gtttgccggc
     1621 aaaatggccg agccagtacg tgaaggctca agcgggccga ttgcgccgca gggcctgact
     1681 ttggagcata tcgcatatcc cgccgatgat cagctggccg caagagccga ggccattcgg
     1741 gccgtacgta cgttgtagat tctatgaaaa caacgcaaaa gggctggaac ccaaacggat
     1801 tccagccctt tcagctagct tagcgaaacg ctattcagct cactcggcct tctcggcgtc
     1861 agcggcttcc tcagcgggag cctcagcctc agcggcggcg gtctcctcga ccggagcctc
     1921 aacagcctca gcagcctcag tggcctcagc ctcaacggcc ggagcttctt cttcggcaac
     1981 cttggcggcg gcttcagctt ccttcacaac agccttcttg gcaaccggct cggtgacgag
     2041 ctcgatcaca gcggccggag cggcatcgcc aacgcgcgga gcgatcttga cgatacgggt
     2101 gtagccacct tcacgctgct ccatctgctc ggcaatctgg gtgaacagga tgtgcaccac
     2161 ggacttgttg cggatgacgc gcatcacgcg acggcgggag tgcagatcac cacgcttggc
     2221 gaaggtgatc aggcgctctg ccagcggacg aaggcgcttg gccttcggca gcgtggtggt
     2281 gatgcggccg ttctggaaca ggctggtcgc catgtttgcc agcatgaggc gctcgtgtgc
     2341 ggggctggaa gccagacgcg ggcccttctt aggtgtaggc attgggttta ctccttatat
     2401 gtccgttctt caggcaagca ggtgggcata tgcccgaaca tctgcttgcc tagtgaacga
     2461 acgattggtc aaagaggcag atcactcgtc ttccggcgag aagaacgtgc caccttcgag
     2521 attattggta tcgaaggcca tcggcgagga cttgaggctc aggcccaagg tctgcagctt
     2581 ctccttgacc tcatcgattg acttcatacc gaaattgcgg atgtcgagca ggtcctgctc
     2641 ggtgtgggag acaagctcac cgatggtgtg gatgccctcg cgcttgaggc agttgtaaga
     2701 acgctgcgtc aggttgaggt cctcaatcgg aacggccatc tcggggttgg tctcctcggc
     2761 caccggggcc ggaccgacct cgacgccttc agcctgggcg ttgagctcac ggcacaggcc
     2821 gaagagctcc accaaggtgg aaccggcgga agccaccgcg tcacgcgggg agatggcggg
     2881 cttggtctcc acgtccagga tgagcttgtc gaagtccgtg cgctgttcca cacgggtggc
     2941 ctcgaccttg tagctcacct tgagtaccgg ggagtagatg gaatcaaccg gaatacggcc
     3001 gatctcatcg ttgtcctgct tgttcatctg ggcaggaaca tagccacggc cacgctcgac
     3061 agtgaactcg atctcgagtt cgccatcgtc ggcgagggta gcgatgtgct gctccgggtt
     3121 ggcaatggtg acgccggccg gcggggtgat gtccccagcc acggcttcgc ccttgccgct
     3181 cttgcgcaga tacatgacca caggctcgtc gtattcgctg gtgagcacga tgcccttgat
     3241 gttcagcagg atctcggtga cgtcttcctg gacacccggc agagtggtga actcatgcag
     3301 ggcaccggaa atacgcacgg aggtcacggc agcacccggg atggagctca gcagcgtgcg
     3361 acgcagcgag ttgccgagcg tgtagccgaa gccaggctcg agcggctcga tggtgaagcg
     3421 ggagcgctgc ggattcagag attcctcggt aagagtcgga cgctgtgcaa taagcactgt
     3481 ggttatcctt tcagcttgaa ctcggtggtg cactcaccgg tcaggcggtt ttggatggat
     3541 ttggtattgc tgagtgctca gacgcgacga cgcttcggag gacgaacgcc gttgtgggct
     3601 tgcggggtga cgtcggtgat ggaaccaacc tcgaggcccg cagactgcag ggaacggatg
     3661 gcggtttcac gaccggaacc cgggcccttg acgaacacgt cgaccttctt gacgccgtgt
     3721 tccatcgcct tgcgggcggc ggac
//
LOCUS       DAWBMW010000155         3734 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_5117_length_3734_cov_2.349823, whole genome shotgun sequence.
ACCESSION   DAWBMW010000155 DAWBMW010000000
VERSION     DAWBMW010000155.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3734)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3734
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5117_length_3734_cov_2.349823"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          791 a         1071 c         1053 g          819 t
ORIGIN      
        1 gcccacgaat gcggagacca gcgacttggc ggtggccacg aagatgttgc ccgggccggt
       61 gatcttgtca accggatcgc acaggatgtc gccgtcctgc ggctcgctgc ccttggcgcc
      121 gtatgcgaac atggcaatgg cctgagcgcc gccgaccgcg tatacctcgt caacaccgag
      181 gatggcgcag gtggcgagga tggtcttgtt cggcaggcct tcctcattgt cgcgggcggg
      241 cggggtggcg atggccagcg attcgacgcc ggcggcctgg gccggcacag cgttcatgat
      301 gacggagctc gggtagacgg ccttgccgcc gggcacatac aggccgacgc gctgaatcgg
      361 aatccagcgt tcggccacgc gggcgccttc ggcgaggtcg gtgtagaagt ccttcggcac
      421 ctggttggcg gccacggcgc gggcgcggcg caccgattcc tcgatggcgg cgcgcacttc
      481 ggggtcgagc gtggtcaggg cgtccttgat cgcctcgacc ggcacgcgca ggttcttggg
      541 acgcacgtga tcgaacttct cctcgaagtc gcgcagggca gcggcgccgc gttctttgac
      601 atcgtccaga atcggacgca ccaaatcggt ggcttcggaa gtacccattg cagcacgcgg
      661 catcgcggca agcagttcgg cacgggacag gttctgacca cgcaggtcaa tgattcgcat
      721 gatgttctca ctcataacgc cccatcgtag caatgtgcga ggcctaagcg gcgaggaacg
      781 cccatgttca cccgtatcgt gagattatcg tgcgtgatgt gggttcgtga tacaggttat
      841 tgacgcacgc caaatgcccg agcggtctca tcgataatcg gcatgtcatg cacggcgacg
      901 tgactcactt cggaccatcg cgggccaatg gctaattgct caaccaaggc ttgcactgcg
      961 tcgctgctgc cttgtgcttc cagctcaacc gagccatcgc gacaattacg cacccaaccc
     1021 cgtacaccaa gccgacgtgc ctgagtcacc gtaaaataac ggaatcccac gccttgcacc
     1081 agtccgttga ccactatatg tttgcggacc aaattcgccg attgcctcat gatgctcagt
     1141 tcctaatcca tcgttcgcag cattgggtag aggctcagga atcgccggta tgcgtcggca
     1201 tatgtgcgtg cggtgtcagg attcggctgg tatacagtgc attcgcatac tgattgcagc
     1261 aggtctggta cattgaatac caacgaggcc attgccactg ctggcagaat atcggcattg
     1321 gcgaacactt cgatgggatg ctccaacaca tcggccagaa tctggcacca cgcattttct
     1381 ctggctccgc cgccaatcag ggaaatctcg gtgggtttgg cgtcaaaact ttccatacct
     1441 tgacgaatgg agaaggccac accctcaagc gcggcacgca tcatgtctgc cttggcggtg
     1501 tctggatcga ggcccacata ggcaccgcga atattcggat tcataacagg gaatcgctcc
     1561 cccaccagat atggcaggca tagcacaccg ttggagccgg gtttggatgt ttcgataagc
     1621 tgatgcgcct ctgcgtaatc gccatcggag aatacgccgg taacccattt atgcacgtcg
     1681 ccggcgttga ggaatggcac ggcattcacg aagccttctt ccacgccaaa tgccagattc
     1741 gcagcacctg gcttgtcggt gaacggctcg ggcgaaacgg tggcaatcca gcctgaggtg
     1801 ccgatattga tgttgtattg accagggtgg ctgacaccgc tggcaagtgt ggtggcaccc
     1861 gcgtctccaa tgcccgcgta taccgcagtt ccggcggaaa aaccggtgcg ttgtgcagcg
     1921 gattccgtca ctgtgccaat gttgtcttgc ggattgtgca ggtgtggcaa tatggtcatg
     1981 tcgatttctg cgacctcgca taattcggca tcccactggc cattacggat attcatggcc
     2041 ccagctgtgg aacacgccgc cacatcgccc acgcattcgc cagttaattg ggcgatcaag
     2101 tagtccttgg aactgatcaa tacatgacga atgcgcgcgt acgtttccgg ctcgtgttca
     2161 cgcagccaca tcaacttggg caacggcaag cagccttcga gccggttacc aacggtgttc
     2221 aggaaacgtt ctgcaccgcc ctgatatgtc tcggcgagtt gcttggcctg ttcctcggct
     2281 cggccatccg aatacagaat ggcattgcgg acgggattga gctcgccgtt caaaacaata
     2341 acatcctgca tctgaccgct gaaaatgatg ccacgaatgc gagtcacatt gaattctgac
     2401 tcggattgcc gcgcctgctc aatcatgtgc ctggaggccg agcagaaagc ttgccaccat
     2461 tgatcggggc tctgctcccg atagtcgccg ttgataataa ggtcaagttc gctactcgca
     2521 ctagctacaa tgcaaccctg cccattaact aaggcaccct tcaacgccgt ggtccccgca
     2581 tcaaatgcag ccacatattc cgtagtcata tcgttcatcg tagcgctgaa aaaaagaatc
     2641 attctttgcc agcaaaacgc ggcttattgc atcgtcttgt ttctagaatg ggaaattgaa
     2701 cgaatactac gactagtgag gcattatggc aattcaatcg acaatcatgc tgaacaacgg
     2761 cactgtcatc ccgcaggtgg gcttgggcgt attccagaca ccggatggtg atactacagt
     2821 gaacgcggtg cagacggctc tggagaatgg ctaccgccat atcgataccg caatgattta
     2881 ccacaacgag gcttcagtgg gtgaaggtat tcgccgtgct ggtgtgccgc gcagcgattt
     2941 cttcttgacc accaagctgt ggaatgacga tattcgcgca cgccgcggca aggatgcatt
     3001 ccaagaaagc ctcgaccgct tgggcgtgga ttatgtggat ctgtatctga ttcactggcc
     3061 agcagacggc tggcagcagg catgggacga cttgcaggag atttatgcct ctggccgtgc
     3121 caaggccatt ggcgtcagca acttccaaaa gcatcatatc gatgagctac tgacccatag
     3181 cgatgtggtg cctgctgtag accaggttga gtcttcccca caattcacca atcaagagct
     3241 tattggccag ctgcgtgcca agggcattca cactgaagct tggagtccgc tcggcggcac
     3301 cggcggtaac ctactcagca acccagtact ggccggaatc ggtgccaaat acggcaggtc
     3361 tgctgcacag gtagtgattc gttggcacat ccagcgcggc atcgtcgtgc ttcccaagtc
     3421 cactcatgcc gagcgcatca agcagaactt cgaggtcttc gacttcaacc tatcggtcga
     3481 tgatatgacc gccatctccg ccatcaacac cggcaagcgc aacggtgctg atccggacaa
     3541 tttcaatttc tagaccttta tcggtccaga tacgcaaaga gggaactctc cgtcattgga
     3601 gggttccctc ttctatatgc tttcgattcg gccacaccct aggtgcgaga gcctatgtct
     3661 tgtcggtgtc ttgtcttgtt ggtttcgggt atgcgaaagc cccttggggc gtgtgctccg
     3721 aggggcttcc tgta
//
LOCUS       DAWBMW010000156         3717 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_5148_length_3717_cov_2.598853, whole genome shotgun sequence.
ACCESSION   DAWBMW010000156 DAWBMW010000000
VERSION     DAWBMW010000156.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3717)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5148_length_3717_cov_2.598853"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          672 a         1049 c         1167 g          829 t
ORIGIN      
        1 tatgtcagga attcctcggt gatcgaggag agggaaactt cggtcagctc gagtctgttg
       61 ttggcgatca tgccgagcaa ggcatcaaac ggaccggaat agacttcgag gttgacccgg
      121 aagccggaag tggcggattc ctccgtgctg atttcggctt ccgcggagct cccctcagcc
      181 tcgctacgcg agccagcttc cctcagagag gggagccgag aaagggaagt ctcattcatc
      241 gtttatcgaa acaatcgtta cgccacgatg ccgcgagcaa tgagctcacg tgccacctca
      301 cggtattcct ttgatgtctt gtgctcaggg gcatagacca ccacgggcgc ggcggcaacc
      361 gtggaatcgg gcagtttgat ggagcgggag atgaacgtgt ggaacacctt ttcgctgaac
      421 gcctcgtaaa cgcgctggca gacctcttcg cagtgcagtg tcttggtgta catggtgatg
      481 agcacgccat ccacttccaa tgcgggattg atgcgggact gcactttttc gatggactgc
      541 ataagcaggg ccacaccgcg caaggcgaag aattcggcgg ccacgggaat gatcacgcca
      601 tcagctgcgg caagcgcgtt cacggtgagc aggccgagcg atggctggca gtcgacgatg
      661 atgagatcgt attccgactt gagcttgcgc agcacgctgt tcaggatctg ctctcggccc
      721 acttcggtga ccagctggac ctcggcggcg gacaaatcga tgttcgccgg aatcacatcg
      781 atgttctcga atgcggtgtg ctgcaccaca tcatgtgggt ccacggaaat atcgaacagc
      841 gcggtgtaaa tcgtgttctc cacggtattc gcgttgacgc ccagtcccac agtggcggca
      901 ccctgcggat cgaaatcgac gataagcacg cggcggccat actgggccaa cgcaccggca
      961 atattgatgg agctggtggt cttacctacg ccacccttct ggttgcacat ggcaatgaca
     1021 cgcgccggac cgtgctgttg cagcggttcg ggtgcgggga aggtttcgta ttcacgtcca
     1081 agcaaatcgg taggcatact gtctacctta tcaacatccg ggttcgctgg gggtgcaacg
     1141 acatacgttc ctgtcatagg cagcgttcct tgacggaatg cgctgtcttt atcgcgggta
     1201 tctcgccagt cgtccatcgt tgccctcgcc ccctttgtcg ggaatacctg ctcgcccatc
     1261 ctaatgtact cgccgggctc gcctcggaac accgttttgc cgctggtatt ccagtgttcc
     1321 gaggtttatg gatctcgtct cgtactatgt cgatgcgctt actcggtgcg cagtgcggtg
     1381 gccgggtctt gcttggctgc cttacgcgag ggaatcaggc cgccgatcag cgtgagcacc
     1441 acgcttagga tcaccagcac cacgccaccg gtcaccggca gggcggcatt gacgtccgca
     1501 ttgccgatga agtggtgtag cacggcgttg atcggaatat tgagcagcac ggtcaagccc
     1561 acgcccaaca ggccggagca taggccgatg atgccggtct ccgcattgaa cacctgggat
     1621 acgttgtgct tcgaggcacc cattgcgcgt aggatgccga tttccttggt gcgctccagc
     1681 accgaaatgt aggtgatgat gccgatcatg atcgaggaga cgatcagcga gacggacacg
     1741 aacgcgataa gcacgtagga aatcacgttg acgatggtgg tcaccgagct catcatgagc
     1801 ccaacgtaat ccgtgtaggt gatcttgtct ttcttcgaga cggtgttgtt gtagtcggtg
     1861 atcgcgtcgg cgatgtggtc cttgtcttcg aagctgtcgg cgtagatgtt gatcgagctg
     1921 ggcgcgtcac gactcaccac accgaatgcg gcgaggttgt cgtcgtaggt gccggtcgac
     1981 acgtactggt cgtagatcgc gacaagcacg tcgttcgaag ccgtggcgat atacgcgtcg
     2041 aaactgtcgg cgagctgctg ttcgctcatc gcggccatgg cggcggcctg atcgccggca
     2101 cccgccgcgc cagcgacacc tgcagcacca gcaccagcgg cgtccgatgc gcccgtgccg
     2161 gactgctgcc cggcgctcgt catcgaattg gccatgttcg ccttctgagt cacgcccagc
     2221 gaagccacgt aggtgcgcgc gtcggccgcc ttggtggcgt cgtccgaggg cgagaacgtc
     2281 atgccgttga gcacgttcgt gttcgggttg gccttctgat cggtgacgat ggcgctggag
     2341 gccgcacggt cgatcagatc attggtcaga gcgcgcgtgt atcccacgcc agcggccaac
     2401 ggcgtggccg aagcatcctt cttcgccttg acgacgccca ccaccttgag tttggtggcc
     2461 gtttcgaggc gctgttcgat ttcgtccttg tcgttgccga tgaatcggta gtggccgtca
     2521 tcgcccttga cgtactggtc ggatgccgga atcatgtaca gcgtctggtc gagggcctta
     2581 gcatagtcga tcttgtcttg tggagtgctg accttgtcgc cggcgttgag cttggacatc
     2641 atctcgtggt agtcggaggc gggcagcagg ccgagctcgt acagcgtggt cagcggcacg
     2701 ctgttgttgt cgtcgagcac cagcaccact tcatccttgg acttcggcca tgaaccgttg
     2761 accacttggt agttgtcggt gatgaccttg cctacgagct tgctgtcgtc cgctcccggc
     2821 atgatctcgt tgaacgagtc gggcgcggcg ttctcatcgg ttttgccggt cagcatcgac
     2881 atctgctgcg aggtgatcga cgaggtgccg gacatgccgg acgagcttgt ggacgccatc
     2941 tgcgaggcca ttgaagtgga gtcgctggat ccgatcgtca cgccgtccgc attgaccagc
     3001 gtgccgtcgg ggtcgtggtc gaacacggag aatttcacgt catatgtgta ctggatgccg
     3061 gtggaaccca catactggtg aatctcgctc ttggaattat ccaggtattt cttgaagcgg
     3121 gtcaggttgt tttcggtaat cgaactggtc aggctggatg cctgcttgac gctgcggtcg
     3181 tccgggtaga tgccgtcggt tttgtgcgtc ttgccgccgt atccgtcatc gccgcccatc
     3241 tgtccgccca tcatgctgct caggtcgaag gtctgggagt cgatggtgat cgggtaggcg
     3301 gtcatcgtgt cacgctggat gtcactgatg tatgtgttca cgccagtcga caccgataga
     3361 atcagcgcga tgccgatgat gccgatcgag ccggcgaacg aggtcagcag ggtgcgcgcc
     3421 ttcttggttt tcagattgtt gaagctcaac gccagcgacg tggcgaacga catcgacgcg
     3481 cggcccatcg tcttgtacac ggccggctgt tcggccgtct cgtccacgac gaacgggtcg
     3541 gagtcggagc ggatcacgcc gtcgcgcagg ttgacgatgc gcgtggcata ttgttccgca
     3601 agttcggggt tgtgggtgac catgaccacg aggcggtcct tggccacgtc cttgagcagg
     3661 ttcatgatct gcacggacgt gtccgaatcg agcgcgccgg tcggctcatc gcagagc
//
LOCUS       DAWBMW010000157         3716 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_5151_length_3716_cov_2.588091, whole genome shotgun sequence.
ACCESSION   DAWBMW010000157 DAWBMW010000000
VERSION     DAWBMW010000157.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3716)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3716
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5151_length_3716_cov_2.588091"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2657..2666
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2930..2939
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          724 a         1179 c         1065 g          728 t
ORIGIN      
        1 cagcatcagc gcgccgaaca aggtgccgac gaggaagatc actgcaatgg ccacgtgcac
       61 cagcggtgcg cttcttgttc tggtccattc gatgacccgt ccgacgacat tgccctgctc
      121 gtttccacgc gggttggaga ccacccgcag ctcaggtcgg gaggaggact gatgcttggt
      181 gtgcggtcgg ttcgaggctg gagccgcgtt cgatcgaatg ctacgtgcag ttgccatatg
      241 ctgttatccc ctccttgtaa gaattcgtgc cgtgtttttg gctctgcccg gagacgcgga
      301 gccttgtatc ttgacgtcat tcaggccctg cgcggtttgc gtaaagcgtt tcctccatcg
      361 tgacggaatc tcacggctga cttccaccgc ccgcaaccgc accgaagccg atctcgggtt
      421 ggctgcgatt tcatgttcgt cggccttgat ggcacctcgc gtgagatccg tgaaaaacgg
      481 ttgggcatcc ggcggtatga caggcaggcc agccggaaca tccactcgca gcccttgtgc
      541 catgaacgat ttgacggttt tgtcttcgag cgaatgatag gactccacca ccagtcgtcc
      601 cccgacatgc agtcggttcg cggcctgcgg caatgtggag gcgagcttgt ccaattcgcc
      661 gttgacttca atgcgcaacg cctggaagac gcgtttggcg ggattgcccg ccgggcggtg
      721 ggcttgcggc accacttcat cgaccagacg gttgagctgt gcggtggtgg taaacggctc
      781 cttgtctcga cgcgccacga tggcgcgggc gatctgacgc gagaaacgct cttcaccgta
      841 ttccttgaat atgcggacca gttccgccgc atcataggtg gcaagaatcc gttcggccgt
      901 caacggctgg ctgacgtcca tgcgcatatc cagcggcgca tcatgcgaat aggagaagcc
      961 acgatcggtt tcgtcgatct gcaggctgga taatcccaga tccatgaata cggcgtccac
     1021 gcgttcgatg tcttggtccg ccaacacctg atcgagctga tcgaaggcgg catgcaccgg
     1081 aatgaatcga tcggcgagcc cctcccgttc catacgttcg gtggccagcc ccagcgcttc
     1141 actgtcacgg tcaatgccga tgagccgagc ctgaggcgcg gccttgagga aggcgatgga
     1201 atggccggca aggcccaggg tgcaatccac tgcgatggcg ttctcatgtt ccagggcagg
     1261 tgccatcagg ttcacgcaat cgtcaagcaa taccggcaga tgaatattcg ccacatcaac
     1321 catcagaatt ccacctccgg caacacatcg tcagcaatat cggaatatcc ctcttccttc
     1381 tcggcgaggt aggactcgca cgtgtcctta ttccacagtt cggcacgggt gccaacgccg
     1441 atcaccacga cgtcggaacc cagattggcg taatcgcgca gcatctgcgg caccagcacg
     1501 cggccctgct tgtccggctg ctggtcgacg gcaccggaaa ggaacacacg caggtattcg
     1561 cgggcggctt tgtttcccac cgacacacgc tgaatctgcg aggcgatacg tcgaaattcg
     1621 tcgaacggca gcaggtacac gcaccgttcc tggcctcggg ccatgaccag cccctgcccc
     1681 agctgggagc ggaatttggc cggaagcgct acacggccct tggcatcgat cttcggcgta
     1741 tatgtgccga gaagcaatgg cggcagacca gccatgagat cggccatagt ggtggagcct
     1801 tgtgcgttgg catcagcgtc gcgaatcgtc tgatcggcaa ccatggctcc acctccttca
     1861 tcgttacgtg ggatacactt cggccaactg gctcaccact ctaccccacg attccccatt
     1921 tctccccact catgcacgat ttgctcaccc ttttagggca attaccgatg gattgcgcgt
     1981 gtcggccacg actgtcgcac gaatggacgc tccgcacatc caatccacgt cgcgcacccc
     2041 accgaccgag cagcatggtg aattcacgct gaattcacct attccgagtt gtccgcatca
     2101 ctgacgtagg gggtgtgcgg taaatttgaa tgccttaagt ttttctgccc cacggaaatg
     2161 gaacgaagag aacagcatgt cgagttacgc ctcccagctg catgaagagc agcaggccgt
     2221 cgatcgcgcc tatggacgat tggatgatct gcgagccgaa atgtggcaac gcctggacac
     2281 cgtacgcgcc gccggctccc acggatcccc cacgcaacgc agcgaacgag actcctttgc
     2341 cacaatgtac gaaaaccgcc tcacgcaatt gcgcagcgtt gaggatcggc tggtgtttgg
     2401 ccgtctcgat gccaagaacg gcgaccgtca ttacatcggc cgcatcggcc tgtcgagccc
     2461 ggaccatgag ccgattctga ccgactggcg tgccgaagcc gcacgtcctt tctatgaggc
     2521 gaccccttcc aatcacggcg acatcgtgat gcgcaggcat atcaccttga gtttccgcga
     2581 agtcgtgggc gttgaggatg aggtgctcga cgtgcattca gaccaggtcg gccaggcgtc
     2641 ttccgccggc acattgnnnn nnnnnnctgc tggcttcgtt gagctcacgc cgcaccggca
     2701 agatgaccga tatcgtggcc accattcagg ccgagcagga ccgcatcatc cgctccgaca
     2761 tgaatcgcgc cgtggtggtg cagggcggcc ccggcaccgg caagacggcc gtggcgctcc
     2821 atcgcgccgc ctacctgctg tacacgcacc ggcgcacgct ggaacgttcc ggcgtgctgg
     2881 ttgttggccc cagctcggcg ttcctgcact acatcgatca ggtgctaccn nnnnnnnnng
     2941 gtgctacctt ccttgggcga gaccggcgtg gtctcacgca ccatcagcga tctgattccc
     3001 ggcatcaccg ccacggcggt cgactcccct tatgcagcca agctcaaggg cgatcgacgc
     3061 atgacctccg tagtggccaa tgccatcgcc gcacgcgtgc gtgttcccgc tgctctgccg
     3121 acagtaacca ttagcggcat tcaggtacca atgctggcca ctgatatcga gcaggcgcag
     3181 gcggacgcga agcgcacccg acaacctcat aacaaggccc gcgagacctt tatccgttcg
     3241 atgctgacca gcatgcagaa ccgttatgcc gaacagcttg attacacgcc ggatcaggcg
     3301 gagctgaacc gcgcgatgtc gctgctacgt atgaacgagc aggttcgcaa gacactgaac
     3361 ctgtgctggc tgccgatgac cgccccatgg ctcatcgatc agctgttcgc ccatcccgaa
     3421 cgtctgaagt ctctggctgg ttggctgacc gacaacgaca tcgcggcact tgcccgcccc
     3481 aaaggctccc cgttgacccg ctccgatatt ccgctgctcg acgaagcaat ggatatgctg
     3541 ggccccgatc ccaaggcggt gccccgccag tccgccgccg acgcacgtcg tgccgccgaa
     3601 gagcagtatg ccaaagacac tctggccgcg accggtctgg gtggaggcat cgtctccagt
     3661 cagatgctgc tcgaccagat gaacggggac gacgctgagc tgaccgcgca gcgggc
//
LOCUS       DAWBMW010000158         3715 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_5153_length_3715_cov_2.244262, whole genome shotgun sequence.
ACCESSION   DAWBMW010000158 DAWBMW010000000
VERSION     DAWBMW010000158.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3715)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5153_length_3715_cov_2.244262"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          723 a         1192 c         1122 g          678 t
ORIGIN      
        1 catggtcaac atcggcgtgg tcgtgggtgt gttcccggta ttgggcgtgc ctatgccgtt
       61 cgtctccgca ggcggctcat ccatggttat gtgcttgacg gcggccggac tggttgtggg
      121 acttatgcgc tctcaaccgc agataaaaca gtcgcgtcag agcgcgtaag tagactcggt
      181 aatcatgaat caaggcacac cacatatcgt tcttgctggc ggcggtaccg ccggacatgt
      241 caatccgctg ctcgcggtcg ccggggcgat tcgcgacatc gaacccacgg cacaggtcac
      301 cgtcatcggc actgcggtgg gtttggagaa ggatttggtg ccggaggccg gctacgagct
      361 cgacaccatc gaaaaagtgc cgttcccgcg tcgccctaac ctgtacatgc tgcgcttccc
      421 ggcaaaatgg aagcgagaaa ccgccaaggt gcgctccatt cttgaaacgc ggcatgcgga
      481 tgtcgtggcc ggattcggcg ggtatgcttc cgcccccgtc tacgccaccg cacacaagat
      541 gggcattccg attgccatcc atgagcagaa tgcgcgtgcc ggcatggcca ataaactggg
      601 cgctcgttgg gccgatttca tcggtaccgt gtacgagggc accggtctca aaccgcgtgc
      661 cggagccgac gtcgagcgcg tcggtctgcc gttgcgcccg gccatcgcct ccctgaccaa
      721 acgcatcggt gacgaccgtg ccgccgtacg ccgtgaatcg gccgcgcagc ttggcgtcga
      781 tccgaaccgt ccgctggtgc tggtcactgg cggatcgctg ggagcgcaaa gcctgaaccg
      841 cgccatcgcc tcctccgccg cagacctgtt ggcccacgca caaatcatcc acttgaccgg
      901 gcgaggcaaa atcagcgaag tacgcgaact ggtcacggcc tcggccggcg cggacgtgct
      961 taccggcatc ggccccgaat cggccggaca aggtgactac cataccgccg aatatctgga
     1021 acgaatcgat ctggcctttg cctgtgccga cttggtgatt tgccgtgccg gagccggatc
     1081 ggtatccgag ctggctgcac tgggcctgcc ggccatttac gtgccgctgc ccatcggcaa
     1141 cggcgagcag cgtttcaacg ccgagccagt ggtgaacgcc ggcggcggcc ttctggtcgc
     1201 agacaaggac ctgaccccgc agtgggtgca tgagcatgtg cccgatctgc tcgccgatca
     1261 cgaacgactt gccgaattcg gccgcaaggc atgggaatac ggcatcagaa acgctgccga
     1321 aatcatggcc cggcacgttc tgcaattagc cgaaccatcc aagtagtatt cagatatcat
     1381 tccgtgcgcg atactgacca attccgttcc gcagcacgag atacggtcat aatatagggt
     1441 gctggaatcc ctaaaggagt tttgaattgt ctcaagatca gcccatagtg cttgacccta
     1501 cgtatgcctc attcgatgct gcggcccgcc ctgcggatct cggcgccacc catttcatcg
     1561 gtatcggcgg tgccggtatg agcgttctgg ccgaaatgct gcacgccgaa ggagtcgcgg
     1621 tggacggctc cgatcgcgca cacagcgcca aaaccgaccg acttgaaacg ctgggcatca
     1681 ccgttgaatt cgggcagcga gccgaaaatg tcgcccaggc cgagaccgtg gtctactcca
     1741 gcgccatcaa accggacaat ccggaaatcg tcgcagcaca cgcggcgggc aagcgcattg
     1801 tgcatcgcag tgacatcctc gccctgctca tgaacggcaa acgagccgtc accgtggccg
     1861 gagcgcacgg caaaaccacc accagctcca tgctgtccca cattctggtg aacgccggtg
     1921 ccgatccaag ctacgccatc ggcggcttta tccaaggccc ggacggcacc acgctggacg
     1981 gcggacacgc gggcaaaggc gacatcctgg tggccgaagc tgacgaatcg gacggcagct
     2041 tcgccaaata ccatccgacc atcgccatta tcaccaactg tgaagccgat catctcgacc
     2101 attacggcga cgaagcccac taccgtgccg cgttcgttgc ccacgccggc cgggccaccg
     2161 gtcacgtgat catcagtatc gacgaccccg acggacttgc cgtgctggag gctctgcccg
     2221 ccgacgtgaa atcccatacc gttgcctacg gcactaccgc gcgtgagtcc ctgcccgatt
     2281 tgggcggtgc cgcgtatgtc tggatcgctt cggaaagcga gaccgctggc agcggcgtcg
     2341 aacagctcac cctgcatctg ccggccgcgg tgacggcggg ggagccggtg tctcagagcg
     2401 ttgccctgaa ggttcctggc gtgcacaatg cgcgcaatgc ggccgccgcc atcagcgctg
     2461 ccgtgctgct gggcgtgagc cccgcagacg cggccaaggc ggccggcaca ttcctcggtg
     2521 cggcacgccg cttccaggtg cgcggcacgg tcaagcaggt cactgtagtg gacgattacg
     2581 cccatcatcc caccgaaatc gcggccctgc tggatgcggc ccgacgccgg taccccgatt
     2641 cgaccattcg tgtgatcttc cagccacacc tgttctcccg caccaagttc ttcgcgcacc
     2701 agttcgccaa atccttagcc aaagccgatg acgtcattat caccggcatc ttcccggccc
     2761 gtgaaaagca gaccgatttc ccggacatca gcccctccac catcgtggac gcggctgcgg
     2821 gactcaagga tgcttccgca ggcacgtgga ttcagccggt tgaggatatg tgcctggccg
     2881 ccaagatgat ggccatgcgc gcccaccacg gcgacgtgat cttcaccgtg ggcgcgggcg
     2941 acatcaccga catggatcag gtgctgctca ccgcacttga ggcccatcgg gaaagctgcg
     3001 agtgatgtcc gggcgcacgg tcagatccgg gggcaatgac aacggctcac gtcgttcgtc
     3061 tgggagctcc cgcaagccgc gcaccatcgg ttcacgcgga aacacgggtc gctcgtcgaa
     3121 gtctcgcgct ccccggtccg tgtcttccgg tcatgagatc gacgagccgg agatcatcga
     3181 cgatgtgacg gtagccaagg gccaggccgg tgccttcgtc gatgcacggc ggttgcacag
     3241 cgaggattac gttgccgaaa ctctgcatca gaccaccggc tccctaggag tcgcatcacg
     3301 cccgaaagtg gtgaatttca ccgaacgcgc caaagaacgc aagcgtgcaa acgtgcgcgt
     3361 cgttgcgtta cgcgtgttga ttgcggtcgt cagtgtggct gtagtggcgg ggctggcatg
     3421 gctgctgctt ttctcgtcgg tgtttcgtct ggaaaccagc gagatcggcg tatccggtgc
     3481 caacgaatgg gtgagtgcgc agaccatcca tgtcatcgcc gataagcagg ctggaaagtc
     3541 gttgttcttg gtctccgccc atgaggtcac agagcaactc aagtccattc ccggcgtcag
     3601 cgaggccaag gtctccaaac agttccccaa gtcgatgagc gtggaagtca aggcgcaacg
     3661 gcctgcggcc atgctgaaaa gaggcgatac cctgaccgcg gtggattccc aggcg
//
LOCUS       DAWBMW010000159         3713 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_5156_length_3713_cov_2.373428, whole genome shotgun sequence.
ACCESSION   DAWBMW010000159 DAWBMW010000000
VERSION     DAWBMW010000159.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3713)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5156_length_3713_cov_2.373428"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1525..1534
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          751 a         1103 c         1097 g          752 t
ORIGIN      
        1 acttcgaaca cttccaacct tagacaaggt gttgcacttc tatttagaac ccgggctttt
       61 taatgacaaa cgataaatta tggtataatg caagtatgag ttgcttggtt tgcgcaatga
      121 agcggaggct caatcatggc aacgacaaag aaactatcct tcggtttgga taaggccggt
      181 ggcgcggcct tgatcctggc ccgcatcggt gccgcgctcc tatggatcat cgcagcgctc
      241 gcggccgtgg tgtgggtggt attcctcatt tccccgcgct cgttcgccgc gaacttcggc
      301 agtgtcgacc tgaatgtcgg cggcgcgttc accctcagca tcaagggcga cttgttggac
      361 tactcctata atctgcagaa cttccacgac ggtcccgtca acatggtagc taccatctcc
      421 accggtctgg cgagaatggt ggtctacgct ctactggcct tggcgttctc cgaaatggcc
      481 gcactgtgca aggatttgac ggtgtggctt gcagacggca gggatgccac gcctttccaa
      541 ggcatggtgt ccgaccggtt gaacagggct ggctggtatc tgattgtcgc gccgattgtg
      601 ggtctggtga acttcatcgt atgtttcctg gctggcacgg gcggcaactg gggcttattg
      661 atgattggca ttatcctgtt gctgattgcg cgcatcttcg attatggcat gggtctgcaa
      721 cgggaagtcg acggtctgct gtgaggggga agagcgccgt aggcccgcgt ggcgccggtc
      781 cgaacagggc cggtccgaac accgagggcc cgaatgagga cagcctgaac atcgttgatt
      841 cgagcgagac cggcctgaat acggctgact caaactccac cggtccggcc ggcataggcc
      901 atatcgtcct gcgcctcgac cgcatcatgg tcgagcgagg ccgctcactc aactggctgg
      961 ccgatcaggt gggcatcacc aacgtgaacc tttccaaaat caaaaacaat cgcgtttccg
     1021 ccatccgctt ttccgccctc gccgccatct gtgcggcatt ggattgccag cccggcgaca
     1081 ttcttgaata tgtggatggc atttcgggcg atgcggacga tgcgaatagc aagtaatcgc
     1141 acgcatgcca actcttctcc tcctggcctg tgattgattg acaatcaggg attacatgcc
     1201 aaatgggaga gataaacatg tggaggttgg attggatggg tgcagaagtt gcgaattggc
     1261 cccatccaaa ttggactggt ttgggtgggg ccaatccact ccgttcaata atgaagcgcg
     1321 ataccaagca gcgtcgaacg aagggaaact catgacgcag aatcgtgcgg aactcaaccg
     1381 taatttggcc cagatgctca agggcggcgt gatcatggac gtcaccacgc ccgaacaggc
     1441 gaaaatcgcg caggatgccg gcgcgtgcgc ggtgatggcg ctggagcgca tccccgctga
     1501 tattcgcgcc gccggcggcg tctcnnnnnn nnnngccatg attcgcacca agggcgagcc
     1561 cggcaccggc gacgtgattc aggccgtgcg ccacatgcgc accatgaaca agcagatccg
     1621 cgaacttgtc ggcctgcgcg acgacgaggt ctacgaggcc gccaagcagc tggccgtgcc
     1681 ctacgatctg gccaagtacg tgcacgacaa cggccgtctg cccgtcgtga acttcgccgc
     1741 cggtggtgtg gccaccccgg ccgacgccgc cctgatgatg gaactcggtg ccgaaggcgt
     1801 gttcgtgggc tccggtatct tcaagtccgg cgatccggcc aagcgcgccg ccgccatcgt
     1861 caaggccact gccaactggc aggacgccga tctgctcgcc aagctgtccg aaaacctcgg
     1921 cgaggccatg gtcggtatca acgaggacga gattcagacc atcatggccg ctcgcggaga
     1981 gtgacgggag tcgaagccgg agaggacaac atggttgtag ctgttgaata catttccaaa
     2041 gaagaatccg cagacgccga aaccgccacg cacggcgtga ccggtattat ggccgtgcag
     2101 ggcgcgttcg ccgaacatgc ggcggtgctc gacaaactcg gcgccccgtg gaagctgttg
     2161 cgcgcggccg aggatttcga tgaatccatc gaccgggtca tcctgcctgg cggtgaatcc
     2221 accacgcaag gcaaactcct gcgttccacc ggcctgttcg agccgatcgc cgcccacatc
     2281 aaggccggca aaccggtgct tggcacctgc gctggcatga ttctgctggc caagaagctc
     2341 gacaatgatg agaacgtcta cttcggcgcg ctcgacgctg tagtgcgccg caatgcttac
     2401 ggtcgtcagc tcggcagctt ccaagccatc gctgatttcg gccaagccga cgacccacag
     2461 cgcatcgcgg actttccgct ggtgttcatc cgtggaccct acgtggtgtc ggtcggcccc
     2521 gaggctacgg tcgaaaccga agtcgatggt cacgtggtgg gcctgcgcca aggcaacatc
     2581 ctcgccaccg ccttccatcc cgaactcacg gacgacgtcc gcatccacga gctcttcctg
     2641 tcgctgtagc cgattcgtct gccgttagac tggtattcga ccgacgaatc aaggagacga
     2701 tctgccatgc agttcacgct gaaccggcgc agcacggtgc cgatgtaccg gcagctggct
     2761 tctgagctgc gtgaacagat tgtctccggc gcaatggctg agggctatcg tctgccgcct
     2821 gaacgcgagt tggcctcacg cctcgaggtc aatcgcacca cggtattaca ggcctatcag
     2881 caactcaaag atgagggact catcgcctcc aaagtaggca aaggcacgtt cgtgctgccg
     2941 ttgcagcata ttgagactgc ccggattcct gtagtgagac atgagccgat tgacgaggag
     3001 ccgaaaccga gcgttgtgcg gccgccgtgg tcggtgttgt tctccgacta ttccaaccga
     3061 ttcacctacc acgacattgc ctcggccgag cgggcgcaac gcggcgatac catcatcgac
     3121 tttgccaccg gctcgcctaa tccggcggat attcccgacg atttgttgcg ttcagtgagc
     3181 attgaagcgt ttgagtcgcg cgaattcgac ggtcagccag aatcgcctat cgaaggattc
     3241 gatgaactcc gcgcattact tgccgagcat atgagcacac gcggcgtgca gtgcgatgta
     3301 cgaaacgtga tggtgctttc cggttccgag caaggcatcg atcttatcgt gcgtgcattc
     3361 gtcaatcctg gcgactgcgt gctggtcgaa caatccacgt tcttcccggc attgcagacc
     3421 ttccgctcgg cggatgcgcg catcatcggc gttccagtgg acgagcacgg tatgcgtaca
     3481 gacctgttgg acggctattg cgcgcgattc cggcccaagc tcatctacac cgtcccgacg
     3541 ttccaaaacc ccaccggtac cacgctgccg cccgaacgac gcagcgaact catcgacgtg
     3601 gcgcggcgct atcagtgtct gatcgtggaa gacgatgctt acggcgattt gtggtacggc
     3661 ggggaatcgt ccgacgcaca tcgccccata ccgcttaagg gcatggagaa cgc
//
LOCUS       DAWBMW010000160         3703 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_5166_length_3703_cov_1.864309, whole genome shotgun sequence.
ACCESSION   DAWBMW010000160 DAWBMW010000000
VERSION     DAWBMW010000160.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3703)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3703
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5166_length_3703_cov_1.864309"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1923..1932
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          826 a         1155 c         1082 g          630 t
ORIGIN      
        1 tcccaacgcg actcgctgac ttcccagcgc gacgatgccc agcaacagct ggacgcattg
       61 cgcagcgaag cggactccat tgccgacgat gacggtgccg cattggacgc cgcacgtgca
      121 acgctcgccg aatgccgcga acgactgaat gaacgcgccg ataagcagcg cgagatacag
      181 tccaagatca tctcgctcaa atccaaggcc gatgcgcttg ccgacacgct agacagccgc
      241 aatgcctccg gatcgttgga gcgcgatacg gacgtggcct cgcttggccg actcaccgac
      301 ttcatccatg tggccgaagg ctgggaagaa gccgtggccc acgcgctcga ccagtatgcc
      361 agcgccatcg tggtacccgc ggcaggcaac atgttgcacg ccctcgaacg cgctcgggaa
      421 gataagctcg gcaaagccgt ggtgctgacc gcgagcattg ccggcgatcc cgccacagag
      481 ccgggcacgg agtcggagga gagtagcgcc gagaacgcgg gggccgcccc gcaatcgggc
      541 aacgcgctgg cagcactcgt caccgcgaac ccggatgccg agaacccggc acaggccgaa
      601 gcggtcgtgc gcaccgtgcg tctcctgctg gctgatgtag ccgcggccgg caccgccgac
      661 gaagcccaac agattgtggc attcggcgaa gccatgcggg ccgtcaccaa gaacggcgag
      721 accttcacac acggcgtggc ggcggtcggc ggctcttcaa tctctcaatc ggacctgtct
      781 ctggccgccc gccgagacaa agcattggca caggtcaaac agttgaccgc ccaagacggc
      841 ggaatggctg agcaagtggc cgaggccaaa accaagcgcg atgaagcggc tcgactcgtc
      901 gaccaggaat ccgccaaacg caccgaagcc cggctcaaag cccagcaggc ggagaaatct
      961 ctgaaatccg ccacagatcg cgtcgcatcg ttcacccgtc agctcgagca gctggatagg
     1021 aaaatcaccg agacccaaga gaattgcaac gagcatcagc tcaagcttga cgacctcaat
     1081 cgcgctttgg catcggccca gcaatccaca gcggaacatg ccgatttcga cgaactcgat
     1141 gaacgcgaac gcacgctgga gcgcgaactg aacctgactc gcgagcatga ggtcgccgca
     1201 aaaatcgcgt ggatcgaagc aagccgcaaa ggggagtcac tgagccgaca ggccgggctg
     1261 ctgcgggaca acgccaaaga aaccgccgaa cgccgcgccc gcatcgaggc gttgaacgat
     1321 cgcagacgcg agcaggccgc acacctgcaa ggcgtggccg atgacgcgcg agcagtggcc
     1381 gccatggtag agcatacgct gcatgacgtg gccgccaaac gagacgagct gcaggccgcc
     1441 gcctccagcc atgacgagga actcaaggcc ctgcgcgcgc agcgcaacca gatcgagccc
     1501 aaggtcaccg atctgaccgg gcgcgaacat gccttggatg taaaccgcga acggttggcc
     1561 gccgaatccg ggcagctcat gcaaaaagtg tccgtgatta cggccccgaa cagccggtgc
     1621 ccgtgctgga tgatgagggc aaccctgtgc cgttggatga gacggaagct gaggctgaga
     1681 ccgaagaggg agaaaccggg ggagagaccg gcaccaacgg cgattccgac cgctttaaga
     1741 ccgtgccgta taaccgtcag gagcagaaga agcgtctcga taaggctcgc cgcgatctgg
     1801 ccgcactcgg caagatcaac cctttggcca ccgaggaatt cgaagcgctt gaggagcgga
     1861 acaagtatct caatgatcag cgcaacgatg tggtcaaatc acgtgacgat ttgatgcagc
     1921 tcnnnnnnnn nntcatcaag gatctcgatt ccacgatggt ccaggtattc aaatccgcgt
     1981 tcgacgatac cgccgaggca ttcgagcaga tgttcgccac gctgttccca ggcggcaccg
     2041 ggcgactgcg cctggaaaac ccggaagaca tgctcaccac cggcgtactg gtcgaggcca
     2101 gcccggccgg caagcgtgtc aaacagctga gcctcctgtc cggcggcgaa cgttcactga
     2161 ccgcgctggc gttgctgttc gcgattttca ccgcccgccc cagcccgttc tatgtgatgg
     2221 acgaggtcga ggccgcgttg gacgacgtga acctgacccg tttgattaat gcgttcaatg
     2281 agctacgcgc ccatgcgcag ctcatcatta ttactcacca gcaacgcacc atgtcgattg
     2341 ccgacgcgct ctacggcgta accatgcgag ccgacggcgt aacagccgtg gtgagccaaa
     2401 aactcgaacg ataaatgaat ggttcctgct ggtaggaact ggcgccacag gtgacagagg
     2461 gtggtccatc tcgaaagacc accctcagtc tcacttcgtt cgacagctcc cgccagcggg
     2521 agcggggagg tattagcgca atgccttgcg cacgccctgt acagtctgca gagtcttgta
     2581 gaagaccttc ttgatgggta cgtcgcgggc gggggccacc ggggtgattt cctcggtgaa
     2641 cttgcacttg aactcgttaa gccccttgag cgacggcgcg aaatcggagc cgatgcccat
     2701 gagatcgtat tcagtaatac cctgcgagcc caggatgcag cattcggaat acagcagctt
     2761 gtcggtcaca tgcagacgca taacctcgtt gcgcatgccg gcatagtagc gcaccgcatg
     2821 cgtgccgttc actgtgacaa tcgaccatgc caccacacgg tcttcaatac gtgcggcgaa
     2881 cacacggcaa tgatcggcac ccaacgcgcc gatcatatcg gagtagtcgc tcatcggagc
     2941 aggcgtgaat ccatcgcgct ggccggtctc gaccatcaca tcgtaatatt cggagaaatc
     3001 ggccagcgcc ttatccgtct catcggcaac ctcggccggg cactcgcgca gagacttgcg
     3061 cacgtcacgg cgtccacgac gcttcatccg agccagaatc tcatcatcgc caccggtgac
     3121 gtcgatgacc acggtctggt cgtacggcac tgtggacagc accttctccg taccatcggc
     3181 gaaccaggtg tcgatacgca ggaacgcgat gttcttgtcg gccttcttga cggtgtccac
     3241 gatggcgtcg acgacctcgc gctcctcagc ctcggtgggc ttggcgaccc acgccggccc
     3301 atgcatcgag cgcagataat gataaccgtg cgtctcaaaa tcgataaagg agatcacggc
     3361 gaccaattca ccatcacgct tgatcagata atcgccccat ggtgtgcgtc cgtcgatatc
     3421 cgcctgaaaa ccagaccaaa ccttggtctg ttcgataggc aacgtgatgc cggccttctc
     3481 ggcctcggct tccagtgtgg ctccgtcaac tttctccaac gtgatcatta cgattcagct
     3541 atcctttttc gttcgtatcc gtatgtcgtg tttctggttt ggtcgtattg cgcgtgatct
     3601 gatgtgggct aatcgggatc cgatcaggat ttgattggcc ggaatcccgt cacaagccaa
     3661 acatgcgttc gacgacgtga tcgtcgccgt cgaacggcac atg
//
LOCUS       DAWBMW010000161         3702 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_5168_length_3702_cov_2.367151, whole genome shotgun sequence.
ACCESSION   DAWBMW010000161 DAWBMW010000000
VERSION     DAWBMW010000161.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3702)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3702
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5168_length_3702_cov_2.367151"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          856 a         1013 c         1057 g          776 t
ORIGIN      
        1 tcgaacagct gctcaggagc ttcctgacca tcaaacgcgc catcgacacg gatgccagcg
       61 acatccacga ccagcttgac cgcatcaacg ccatgctcaa aggccagcag ttcggtccca
      121 agcacggcag cctgtccctg cacgcggacg tgcgccgtcc cgagcgcacc ttctgggcgc
      181 agctcatccg cgtcatcggc accctgaacg attggaaatc cgccgacgtc aacgattccg
      241 acgcctcgcg caaggctttc gcggcctgcg cgccgatggt cgggctgctg cgcgccgaac
      301 tcgcgcaggt caaggacgcg aacggcgtga aaagctacgg cgcccgcaac ctcgacccgc
      361 gttgccgctc gtccttctac gccatcgtgc atcatgccga cggcccggat gaacgcatca
      421 cctccaccgg cggccgttcg ggcggcgcct tgcaggagct tacctcattc gtatacggcg
      481 cggcgctcat ctacctgctc ggcggcggcc tgaccagcga gccctcgtac accacgctgt
      541 tcctcgacga ggcgctcatc aaggccgacg gccgttacac gcagcgcgcc ctcacggtgc
      601 tgccgcgcct cggattccag gtcatcgtct ccgccccgga aagcaagacc ggcgaaatcc
      661 tcgaggtctc caccaaggcc tacgtgacct acaaggaccc cgacaccggc ctcacctccc
      721 tgcgcgaggc cgtgctgcat cagaatgaca tggacgatga atcatcgtcg gatatattga
      781 aggatgacgg ggttgccgaa tgagcagtcc tggcgtcgcc ggtattaaga cgattcggtt
      841 cgactcgacg atgttcgaaa gcaggtgatg ggaactatgg ccggcatgaa agacatcgct
      901 gcgatcacca catgcgtgaa gaaacatatg cgttcacata tgtacgacat cgaaccggca
      961 tggccatttc ccgtgccggt gggattgccg gaccaagcat ttttggaaac caatgcgatt
     1021 gcggtgcatg acaacaacaa tgagatacgg caatgggcaa gcaagaatgg ttgcgaaatc
     1081 ataaccaagc atcgcaccat aggcacctct gtcgagctga tctccaaagt cgtcgttcct
     1141 gacgaatcaa tagccatgcg tgtggttggt cgtacgctgg cggcggaatg tcgcgaagcg
     1201 cgccgccgca tagcatgtct ggttgctgct tgtgacgttg ctccggaaac aatcgaatcg
     1261 gccgctcgca tgacaggcca tgaacagcct gacgacttcg atctgcttgt cagcgcagtt
     1321 cgatatttca ggcatcatga cgtgaccggt atgacgccgc gacagattcc actgacagga
     1381 ttcagcggca aatggctgaa tgagtcaaaa accaatcgtc gtaaggccat ttgccgactg
     1441 ctcggtgtcg aaacgttggg tctgtcaaaa cggccgaccg aactgcggtt ccgttatctc
     1501 gatccggtcc gcgacgatgc tgagctcgaa cgcatcatat ggtgtccgtg ggaaggcgaa
     1561 gcgttatccg gcattaaata tgctgtcatc gttgagaaca aagacacgta tcagactatg
     1621 ccgccgattg cgcagggcat ctgcatatgg ggttctggtc gcgctgtaag cgatgctgta
     1681 cctgcggttc ctgcactgcg tgacatgcgt atcgtgtatt ggggtgatat ggatgcggat
     1741 ggactggaaa tactttcgac attacgtgaa tcaggtatcg aatgcgactc catcctgatg
     1801 gattgcgatg catacgatcg gtaccacaga tttggtaccg atcgtactga acgcagcgcg
     1861 aagattgcta tgcgggagcc taagccgacg cccggccttc aatcggcgga gcgacgtctt
     1921 tatgaacgac tgtgtgctga caaaacaatc gcttatcggc gtatcgaaca ggaacgcatt
     1981 cccatgcgtg atgcagttga cgaattacgc aagcttggca tccccgtgga gtccattgaa
     2041 aatcaggatt tgccgattta ggctgagtga ttggggatgt tgccaagagc tagaacggct
     2101 tagctttttc tcgtctcaca ttatgccttc cgattaataa tgcaacggca ttgcatagct
     2161 gcaaggtccg gcactgctat ccgatactat cgaactgttg ttttgcgttg cgcgagaagg
     2221 gacaaacatg gcaaccgctt ggggtgacga gagtgtgcgg aaaaccggag ttccctcgcc
     2281 tatgtacttg atgggcgcat gcgtatgtga tgatacggaa accgaaacaa ggcaaagatt
     2341 ggcgttgctt aagcccaaag gagcaaggaa attgcactgg cgagacatgc ggccatcatt
     2401 gcgtggcaaa gtcgttgatg ccatggctgc aatggacatt gatcatgtca ttgtggctgc
     2461 ggtaccaatg agccagtgga ataccgccga acgcgctcgc cgtaaatgtt tggaaggtct
     2521 tctgcctctg ctggaaacag aatacaacgt ggacacgctt gtgcttgaac ggcgcgagat
     2581 ttcacaggac aggaacgata tccgtttcat cgatggattg agaagccgca gatttatcgg
     2641 gccaattcgt gtcgaactct gtgcagggga gactgatgca aggctatggc tccctgatca
     2701 gctgctagga gcgtacggtg atgcccaggc cggtactgga agatacgatg aattcctcgg
     2761 ccatgttcga acagtgttca tcgacagtga ttgatgtcct aaaaccgtgt atacttcatg
     2821 gtaagccaac cccgctgggg aaatggaagt gagagccgga cgccccgagt atcttggggg
     2881 ccggctcttg cgtttcggtc cactgatggt ccggaatttt gcatactctg gttcagtttt
     2941 ccggcctgac acgccatcga acaagcgttc gaatttggtc gtgtgttcgg gtatccttgg
     3001 gacagatttg gcgcgcgcat gaccgtgcgc gcgggaacgg tgagatggaa ggtggggtgt
     3061 ggctgtggcg gcaatgcaga ctggcgcaag gcgcgcaaca agcgcaacgg gcgcgaaaac
     3121 cagcaagggc aagaacggtg aggtcatcgt cgaaaaggtg atgaccagcg actccttcct
     3181 gagccgggag atcaaaaagg cgccgctgat cgaacacgat ggttcgctgg tagctgatat
     3241 ctcgcacatc ccgaacgatc tcaagatcac gattcagggt gcgcgcgaac acaacctcaa
     3301 gaatgtcgac ctggcgattc cgcgcaatcg catggtcgtg ttcacaggcc tgtccggttc
     3361 gggcaaatcc tcgctcgcgt ttgacaccct gttcgccgaa ggccagcgac gctacgtcga
     3421 atccctatcc gcctacgccc gccacttcct gggtcagatg gacaagcccg acgttgactt
     3481 catcgaagga ctgtccccgg cgatttctat tgaccagaaa tccagaaacc gaaacccacg
     3541 ttctaccgtg ggaaccgtga cagaaattta tgattatttc cgtctgctgt atgcccggat
     3601 cggtattcca cattgtccga aatgtggaaa agagatcaaa aaacagacag tagatcagat
     3661 ggcagatcag atcatgcagc ttccggagcg gacgaagatc ca
//
LOCUS       DAWBMW010000162         3697 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_5180_length_3697_cov_2.594179, whole genome shotgun sequence.
ACCESSION   DAWBMW010000162 DAWBMW010000000
VERSION     DAWBMW010000162.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3697)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5180_length_3697_cov_2.594179"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1907..1916
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          738 a         1042 c         1072 g          835 t
ORIGIN      
        1 tgatttccat cgaagccttg tcggacagcc cgaacttgcc cttgagcccc gcgtccttgc
       61 cgagattctt ggtggcgcgg cacagcgagt agttcgggtc ctccttgttg agccagtagt
      121 actcgtcgag tacgctgacg ccttcggttt cgatggaggg aatcgagcgg aacagatccc
      181 acatgacctc gaaatgattg tccatttcgc ggccgccacg catcacatag ccaaggccgg
      241 gaatatcaag accgtcacag gcgccgccgg gtaccgggtc cttctcaagg atgaggatgt
      301 tctcgccagg catctgacca tcacgcacca gataacaggc tgcagacaat gccgccagtc
      361 cggtaccgat gatgtatgca tgcttgttgt ccacgtcggc cggcttcttc ggacgtgcga
      421 aagcctcata attgccgctg ctgtagtaca tattccgcct ccttggggca gagttgacac
      481 ttcatcaact cttcttgaat agacatcatg tcgcattatt gacgaacgcg ctataggcaa
      541 cgttcggcgt tctgccgcat tcgccaatac cttgcatcta cggttttcga ggcctatgtt
      601 ggctgttggc ccataactat tgaaggcata caggcatact gaggatgcgg ggtgcttcac
      661 cgaaccggat gattgataag gtcccgttca atccacgcca tgaccaaatc ggcaagtcgg
      721 tttacctccg cttcggcgag ttcacggcct ttgggcagat gatgccattt ttcgatgcat
      781 ccgcggcagc atgtggcggt agcatgctga gcggtgaata ccggatggcc gcgccatggc
      841 gtctgtctgc cgtcgttctt cggttcggct gcgcctatgc ggtctcgcaa catttctcgc
      901 gcatgacgat cgatggtcgc cttgcctttg gtgcgggcat aggctcggtc tttgtcggtc
      961 aaagcgaatt tggcgcggaa ctgcgaatga gacagccgtt ccagcatcgt ggcaatccat
     1021 tgttgctcgt catcgttgtt accagtatcc gctatctcca tgcccatacg ttccacagta
     1081 gtcgcgcaca tcgtatggtg gcacacgcct tagctccctc tgatgaagga gcccccagca
     1141 aagccaggtg agggagaggc caggggcgaa acccgccaat caggcggcag gcaagcagct
     1201 ggggccaaac agaatcttga tctcagcaaa caacgaagta tctcgcttga cgcggaaacg
     1261 atcgccaaac gtgagcacct gagcgttacc cttctcatcc agcaccgcaa gatgtacttc
     1321 gcagtaacca ggatgattag cgagcacctg accgagttgc atcatgcgtt ggcgctccaa
     1381 cgccaccgac ggcagcgtga tgaccaacgg acgttcatcc tcggcttcca aggtcggaat
     1441 ctgcatttcc gtggctctca gagaaacggt ttcgtcgcgc aactccactt ggccacgaat
     1501 ctgtacgatg gcgtccacgg caagttcagc ggcggccgct tcatagacct tgccgaagaa
     1561 catgcattgg atggagcttt ccatgtcttc gatggtcacg atggcccagg ggttgccctt
     1621 cttggatacg cgccggtcca cattggtgac caaaccagcc aacgtgacct gctgtctttc
     1681 acccatcgtt ttggctcgat caaccagatg cgcgatggac atttcacgca aggacgcgag
     1741 aatcgactgc ataccggaga gcggatggtc agacacatac aggcccaaca tttcgcgttc
     1801 gaagttaagt ttggtcttct tgtcccattc ctcaagatcg ggtacggtca cggaagcgtc
     1861 gcccatggcc tccgctcccc catcctcggc atcggagaac aaatcgnnnn nnnnnncgaa
     1921 tcgatggcgg cctcgtgcac ggtgagcagc gcgcgacggt tcgggtcaat cgagtcgaag
     1981 gcaccggcct tgatcaacga ttcgaccaat cgccggttca gcgcggtcag gggcacgcgg
     2041 cggatgaagt ccatgaagtt cacgaacttg ccgcgcgggc cttcgcgttc ggcgatgata
     2101 tcggccacgg ccttgtcacc cacgttacgg atggcaccaa gaccgaatcg gacgacgtcg
     2161 ccgactgccg agtactcgta caccgattcg ttgacgtccg gcgagagcac ctggatgccc
     2221 atgcggcggg cctcgcccaa gtacagtgcg gtcttgtctt tgttggtggc ggcaccttgc
     2281 aacagggcgg ccatgaattc gaccgggtag tgcgtcttca gatatgcggt ccagtaggag
     2341 atcagaccgt atgcggcgga gtgcgctttg ttgaaagcgt agccggagaa cggcaccaga
     2401 atatcccaca cggcctggga ggcctcttcg gaatatccat gctccttcat accggcgaag
     2461 aacggcacct tctccttggc cagcacttcg ggcttcttct tacccatggc tcgacgcagc
     2521 acgtctgcct tgcccagtga ataaccggcg aggattcggg cggcggactg cacctgctcc
     2581 tggtagatga tcagaccata ggtctcgtca agcacctgct tcagaggctc ctcaagctca
     2641 gggtgaattg gcgtgatctt ctgcaggccg tttttgcgct tggcgtagtt ggtgtgcgaa
     2701 tccatactca tcgggcccgg tcggtacagg gcgataagtg cggaaatatc gttgaagtta
     2761 tcgggcttca gggttttcag cagtgaacgc atgccgtcgg aatcgagctg gaacacgccg
     2821 agcgtgtctc ctcgggacat gagtttgtag gtttcggcgt catcgagcgg aatcttggtg
     2881 tagtcgatgg cgggtttgcc gttggcctcg atgttcttca gggtgtcgtg gatgatcgtc
     2941 aagttggaca ggccaaggaa gtccatcttg accagcccca acgtttcgca cgtgtggtat
     3001 tcgaaggtgg tggtgatggt gccatcggtg cgttccagca acggcgaggt gtcggtgatg
     3061 ggtgcgctac ccatgatggt ggcgcaggca tgtacaccgg tctgtcggat caggccttcg
     3121 atacccttgg cttcttcggt gatgcgcttg gtgtccgggt cggaatcgta gagttctcgg
     3181 aattccctgg cctcggcata acgtttggcg gcagggtcga agatgtcatg cagactgatg
     3241 tctttgccgc cggtggccgc cggaggcagc gccttggtga ttttttcacc gactgagaat
     3301 tcataaccca tgatgcgggc ggagtctttc agggcctgct tggttttgat ggtgccatag
     3361 atcacgcact gggcgacctt atcggtgccg tatttttcgc cgcagtattc gatgactcgc
     3421 gcacgacctt cagggtcgaa gtcgacatcg atatcaggaa ggctcactcg ctcagggttc
     3481 aggaacctct cgaaaatcag gccatgcttg agcggatcga gctcggtaat gcccatggaa
     3541 taggcgacca tggcgcctgc ggccgagcca cggcccggac ccaccataat gccgtggtcc
     3601 ttggcccact gaatgtagtc ggcgaccacg aggaagtatc cgcagaactg catctggcag
     3661 atcacaccac attcgtagtc ggcctgcttc aatacgt
//
LOCUS       DAWBMW010000163         3664 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_5234_length_3664_cov_2.253533, whole genome shotgun sequence.
ACCESSION   DAWBMW010000163 DAWBMW010000000
VERSION     DAWBMW010000163.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3664)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5234_length_3664_cov_2.253533"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          808 a         1152 c         1048 g          656 t
ORIGIN      
        1 cagcacactg ggggcgaaac caagcatggt gtggaaagcc gtgccgccgt ggggcagcga
       61 ggtgccgatg ccgtgcgcgc actggtcatc gactccgcct attcagacag cacgcccacc
      121 acgctgatgc tcgtcaccca tggctcatgg attaccgcca ccatctccaa cctgctcgaa
      181 cttgacccgg acggcatgaa cgcactcggc ggcatgcgca acgcctgttg gtgccggctc
      241 aaagtgcgcc acagcgtcaa cggcaccccc atcgaacagc ccctgtggga gttggaggaa
      301 tacaacaagg ctcccgccat cgccgacagc gccgactggg agaacgggcc gaccgatctg
      361 cgaggaccgc acatgccaag ttggcagcca atcgtctggt gatggtcgct atgcgaaacc
      421 ccgctcgcaa attcgaacgg tgggtctaga ctaatctcgg tttggaaccg tacagcgata
      481 gaacagtaag aaagcgcgga aggcgggtga gacgacatgc tgagaatctt cagctcaatc
      541 aatggccaag tggagcagat cgagaaaccg gaaaacggct cctggttatg tttgagcgaa
      601 cccactgatg tggagctcgc caccgtcgcc tcgcaaaccg gcatcgacct ggccgacctg
      661 cgtgcccctt tggatgacga ggaacgttca cgtgtagacg tcgaagacga ttacacgatg
      721 atcatcgtcg acattcccac cgtggaagag cgtggcggac gcgactggta cgagaccatc
      781 ccgctgtcga tcatcgtcac cgaaaacctc atcatcaccg tctgcatgca ggacacgccc
      841 gtgctccacc cattcatgga aggcacgatc cgaggcttca acaccttcat gcgttcgcgc
      901 ttcatcctgc agatcctgta ccgcaacgcc acgatgtacc tgcgttacct gcgcatcatc
      961 gaccgcgaaa gcgacaagct tgaactgaag ctgcgccact ccatgcagaa ccgcgagatc
     1021 gtgatgctca tggagctgag caagactttg gtgtatttca ccacatcgct gaaatcgaac
     1081 gaaatcgtga tggagaagct caccaccctg caacgcatca agcaataccc ggacgacgag
     1141 gacctgctcg acgacgtcat caccgaaaac aagcaggcta tcgagatggc caacatttac
     1201 agcggcgttc ttgcgaacat gaccgacgct ttcgcctcga tcgtgtccaa caacctgaac
     1261 aacgtgatgc gcatcttcac catcatctcc atcacactgt ccatcccgac cctgatcttc
     1321 tcgatgtacg gcatgaactt cgatggcggc atgatgggta tgccgttcac tgacaagccg
     1381 tggggtttca tgctcattat cgtgatttcc atggtgctct ccggtgcggt tgcctggttc
     1441 ctcacacgtt cgcgcatgtt caagtaaggc gtttgacggt tatgcaccac tggtcatcgt
     1501 tcgccagccg tagcatgctc cgcgctcgcc gtccgatgcg gcgcggattt cgctcccgac
     1561 tgatggcagg attcgccgcg gcctttggcg tgatattgcc attatccggt ctggccggtt
     1621 gcggccaaat tcaagtcacc accgtctccg gagcgccgga ggggcccacc atcgccattg
     1681 gggtcgcagc cgacgagcca tcggtgggag catggcatga cggcacatac cagggcttcg
     1741 acatcaccat cgccaaatac gtggccgcca aactcggcta tgcgaacaag cagatcgtct
     1801 tcaaacaggt tcgccccgaa aaccggcgct ccatgctgga caatggacag gtcgacatgg
     1861 tcgtcgcctc ttggcctatc accgatgagt ccagcgcgat cgttgatttt gccggaccat
     1921 acctcacgac cagcgtcggt ttgctggtca ggtcggatga gcgtggccga ttcaccaatg
     1981 acgcaggttc ggtgggtgac gtaggcgacg gcacggtgtg cgcagtgaaa ggtgacgaga
     2041 cagtcggcat cttccgcggc aaccacccgc aggcccggat tcaggagcgc agcagttatg
     2101 cgcagtgcgt cacggccgtg caagtagggt cggcggatgc catagcctcc gatatggcgg
     2161 tgctttccgg tcttcaagcg gccaacgggc cccagtatat ggacgtgatt gcggacaagg
     2221 gcgaggtcgg ttatggcatc gccgtgagtc aaggcaccag ccaattggcg cagaaaatcg
     2281 cggaagcatt ggaagacatg gttgaggatg gcagctggac ggccgccaag gatacgttca
     2341 caagccaaac aaagctcacg gtttcgctga acggcgaccc caaagccatc gtctcggatg
     2401 ctgaataacg attgacgacg tgtccagttg cgaggctacc ttgctgattt atgagtgaca
     2461 acgagaagag cacgcaaacc gaagagccca acttccgcta caacgccgca ctggcgcagg
     2521 acatcgaaaa caagtggcag aagatttggg acgagcaagg caccttctgg gccgccaacg
     2581 tcaacggcga tttgaaggac ggcaagggcc gtaacgccga aggccgcacc gcctacttcg
     2641 ccatggacat gtttccctac ccgtccggca agggcctgca cgtcggccac ccgctgggct
     2701 acctggcctc cgacgtggtg agccgctacc accgcatgaa gggcgagaac gtcctgcacg
     2761 ccatgggtta cgacgccttc ggcctgcctg ccgaacagta cgcagtccag accggccagc
     2821 acccgcgcgt gaccactgag gcgaacatcg ccaacatgag ccgccagctg caccgtatgg
     2881 gcctgagctt cgacaaccgt cgtacgttcg ccaccatcga ccccggctac gtgcgctgga
     2941 cccaatggat cttctcgcgc atctacgatt cctggtacga cgaggacgcc accaacccgt
     3001 ccggctccaa gggctccgca cgcccgatcg ccgagctcgt ggccaagttc gaatccggcg
     3061 aaaaggccat tccgggccac gagtccgatg gcaagcagtg gtccgacctc actgacgccg
     3121 agcagcagga tatcttgaac gacttccgcc tggcctacat ctccaagtcc ccggtcaact
     3181 ggtgcccggg tctgggcacc gtgctggcca acgaagaggt caccgccgaa ggtaagtccg
     3241 agcgtggcaa cttcccggtc ttccagcgtg agctacgcca gtggtccatg cgcatcacca
     3301 agtacggcca ccgcctcatc gccgatcttg acggcatcaa ctggcctgag aaggtcaagc
     3361 tcatgcagcg caactggatc ggtgaatccc ttggtgcctc cgtgcacttc atcgtggcca
     3421 ctgccgacgg tgacaaggac atggaaatct acaccacccg tcccgacacg ctgttcggca
     3481 ccacgttcgc cgtggtttcc ccggaacatc acctgctgga gaacgtgccc gccgaatggc
     3541 cggccgatgt acccgaagac tggaagggcg gctatgccaa cccggtcgag gcggtgaagg
     3601 cctaccgtct ggccgccgaa gccaagaccg ccaaggaccg cgtgaacgag gccggtgaga
     3661 agac
//
LOCUS       DAWBMW010000164         3628 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_5312_length_3628_cov_3.054856, whole genome shotgun sequence.
ACCESSION   DAWBMW010000164 DAWBMW010000000
VERSION     DAWBMW010000164.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 3628)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3628)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3628
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5312_length_3628_cov_3.054856"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          787 a          992 c         1083 g          766 t
ORIGIN      
        1 gccagttcgt tgaggcatgg gaggcgctgg tcgaagcccg tgaacgtggg ctggtcaagc
       61 atatcggcgt cagcaacttc ttgcccggcc atattgatct gctcattcgc gatacgggcg
      121 tgaccccggc cgtcaaccaa gttgagctgc acccgtactt ccagcagcgc gaacagctgg
      181 cctacgacaa cgcgcacggc atcatcaccg aggcatggag cccgctgggc cgcgccaacc
      241 agatgctgcg cgacccggac ctgaagcgta tcgccgccaa gcacggtatt tccgtggtcc
      301 aggccatttt gcgctggcac ctccagtacg gcgacgtggc catcccgaag tccttgaacc
      361 cggagcgcca gcgtcagaac ctcgacctga ccgcttttga actcgatgat caggacatgg
      421 ccgatatcgc cgcaatggac aaccccgaag gtcacacctt cggccagaac ccccattggc
      481 acgaagaagc gtgattccag catgcaacgt cctattatga cttcgcggtc gtttttgcgc
      541 acgccgtccg aagcggccgg gcccggcgac gagcaggtga tcgaagacct cgtagacacg
      601 ctggaggcca accgttcacg gtgcgtgggc atggccgcca acatgattgg cgtaggcaaa
      661 cgcatcatcg tctttgtgga tgaagacctt ggcgggcgca taacggtgat gctcaacccc
      721 gtgattaccg cttcggacgg tgccttcgat acccaggaag gatgcctgtc cctgaccggc
      781 gaacgccgca cgctgcgtta tcgacgtatt gaggtcaact acgagaaccg tcggttccgc
      841 gcccgccacg ccaccttcgc cggttggact gcgcagatta tccagcatga agtcgatcac
      901 tgcaacggca tcattatctg attcatatcg gtgctcattg cagcccaact cccgaggctg
      961 gtcatcgcga ttacgatgcg attacatcgc aaccgctatg accagccttt ttgtgcactg
     1021 gtggggtggg tgttctcatc ggtgttgggg tgccatgcgc tggcaggttg gcttcgctga
     1081 ggggtgtggg tggtgtcatg tcgccagcgg ttggctggtt ggcttcgctg gaggttctct
     1141 cagcgtcggt gatgtgccaa agagtggcgg gctggcttcg ctaaggagcg ctaccgtcgg
     1201 tggagaacca gtgcgtgacc tgctgccgac gctaagcgtt cattaagcgt cgttagcggc
     1261 caaaacttgg acgtgtaacg atgctgggcg gttttagcgt cgttgctgtg ccaagttgtg
     1321 gtcctctgcc gacgctgcca gtgcagtagc gtcggcgttg ggacaggtgt tgggcctttg
     1381 ccgactctat aagcgaggga aaatcattcg ccggtgccta agtgggtcta ttgccatgct
     1441 gaatacatct gctgagtgta gctatgcgtt cgaaggcgat ttcgggacct ttctcagtag
     1501 caaatatgcc taatgcgcgg agttatacac ttcaccgtgc acttatacac gccaaacgaa
     1561 gcagaaatcg actgctgaca gtggacgaca cgccagagac gatgtggata acccagagac
     1621 ggtggataac agctgcgcga tcagtggata acctgcaaga aagtgcggtt atccaccgac
     1681 ggtagccact gttatgcaca ttccataccc cttggaatca ttgggttttc cacttatcca
     1741 ccatttcgcc atgtgggctt gcgggccacc gcatgagcgc catacagtgt gcctcacggc
     1801 gggcaacgta gcccgccgtc cacatcacat gaaggggaaa caccatgagc accgcaacca
     1861 ctactactgc cgaaaatgcc ggtttgccag caatgctgga caccaaagac gtagccgaaa
     1921 tgttcaaacg ctgcaatctc gccgtctatg ccgaagcgag acgcatatac tatcgcgaag
     1981 tgaatctgaa cccatgcaaa aagtatccga agcaggtact gcaaaggatc gaatggtggt
     2041 tctgggattg gtttgcctat gactgcgccg tcagcggcgt gggactgacc ggcgacgaat
     2101 ccgaggatct acgcatcgaa ctggaatatg gcccaagcgt aggcatttca ccgtttctgg
     2161 ctttggctga attcatgcat gacagtgact cgtggattgg cgcacgtgaa atacgtgatt
     2221 ttcgcgaact tgacgacacc aatttcgcgt cgatgttctg gattcgtgat gccagcgcgg
     2281 tcacggggcg catcatactc gaagacatga tccacggtgg cgcctacgag gtggcggacc
     2341 cgcatgccgc cgcgcaatat gacggtgcgc acggtggcat gatcgtcaat cgcattgcgc
     2401 atgttcgagg agcatggagg tcatgcgcca tcccgattta tgaggcacgc aggcccgacg
     2461 acccagaaat cggtgattcg ctggttcgca catttcagcg caacggattt aagccggatt
     2521 tcgcggggct cgtgcgattc ttctacgggc gagccaaaga caccggtttc gactgggagg
     2581 atatggaaaa ggcgcgtaat gctggcacgt tggatgagac catcaggaaa gcctacgatc
     2641 gatgaatgaa tcgcttaacg ctgctggccg gggcgacgtc cacggtcgcg aatgaagagg
     2701 gatgcgacca aacagaccgc gcacacaatg gcgaccgtgc ggaacgaagc ccgcactcca
     2761 atggccgatg ccgccgcttc cggcactccg gttcgcatgg ccgccgcggt gtgcgtgttc
     2821 atgatggtga ccagcagcac agccgcagac gtcatgatcg cgaagcccag caaggcgagc
     2881 ggccggccac cgatacggtc atacatcttg cctgccaccg ggttcaggac gcccatggcg
     2941 atgtcgctcg gcatcatcac cagaccggac accagtgcat tatcgccaag atcattccgc
     3001 aaatatgccc atcgcgtaca ggaacagctg gcgcgtggtg aaggtctcga tgaggaacgc
     3061 ggtgatcggg atcatgatgc cgttggtgag catgaatccg gtggtcagcc attgcacggt
     3121 gctgctgggc acatcgaatt catgcatcaa cgtgggcagt gcggaaatca tcagcgtctg
     3181 gttgagaatg gccacaaacg agccgatcat cagggtgatg atcatagcgg tggtgttggc
     3241 aggggtggtg ctgttggagt tggcgggggt gatggagccg gcctcagcgg tggccggcga
     3301 ggaattgcgt gacgtcaatt atcgagcctc gttttcttgg cttgcccata ctatcttgtg
     3361 ccttgactgc attcgaaaac cattgcgatg attggcaaca cgctgagaca cggcgtatta
     3421 attcattcat ttgacaaact tagttcgttc gttttattat tggtcctgca atccgtaata
     3481 tcgcctactg caaaggagcg cccaaatggg tctgtgggat gttgacaaga tcgagtacgt
     3541 cggccgcgcc aaaggaccga aggaagactt cgccttccat tactacgatg ccgacaaggt
     3601 tgttgccggc aagaagatga aggattgg
//
LOCUS       DAWBMW010000165         3570 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_5407_length_3570_cov_3.772688, whole genome shotgun sequence.
ACCESSION   DAWBMW010000165 DAWBMW010000000
VERSION     DAWBMW010000165.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 3570)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3570)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3570
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5407_length_3570_cov_3.772688"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          727 a         1119 c         1037 g          687 t
ORIGIN      
        1 tcacctgacc ctccttgcgg taggtcaggt gttgcaacga tcactagaac ccgcctggca
       61 gcttggcgcc gctggggggg ggagtcatga gtcccattcc aactccccct tattgaccac
      121 ttaggggtgc atcccaacac atggggtgca cccctttttt cttgttgtgc tacggggttc
      181 cagtcgcatt tttttcggtg atattttgaa cgcgaagaca ggctgggaaa tgcccgatag
      241 tcgtgttccc ctcagcaggt gaagccctgc tttcaggctg caaaaccgac tcgggtgttc
      301 cgaaagcggc tttcgttgtt gtacatcgat acataagaga gaacacccat gaaaaacacc
      361 attattcgtg ctgccggtgt gctgttggct gcaagcatga tatttggcgg tgcggcagcc
      421 actgcatcgg ccgcggaagc aacgtcgccg agcaccggca ccaccagcag cacgcaagcc
      481 gcggaatcca tcaatcgcga atgggtgctg aatcctgcga acaacactga gctggcggct
      541 ctcggcacta tacaaggcgg atcccagacg ttcgacgggc tgaccatcga tgccactgca
      601 aacggtgcca agtttgcgcc gcgcgccaat gacacccaga tcaacgccgg aaccatcatc
      661 agcatcccgg tgccagccca caccaataca gccaccatca cccttgcgtt gtccggcggc
      721 accaccacta ttaccgcgga tgccggtgaa gtgagcagcg acaacacagt tatcgccttg
      781 cctgctgcgg agtcggctcg tacggtaaac gtcaccttca ctgcccagac ctatctcaac
      841 ggcatcgccc ttgccgaaca gcagccggaa cccgagttcc ccggtacgcc cacggtcgcg
      901 gccaaagaca cgaactggga cttcacggaa accggcatcg atcgaccggc cgtgcagggc
      961 ctaccgacaa ctaccaaggc gtcgccatcg acgctcgggc gcaaggcgcc aagttcaacc
     1021 cgcgtactac cgcggcaagc ggcggcagcg atactcaggt gaacgctgga accatcctgt
     1081 acattccggt cgcacaggcc gagggcggcg ctacagtcac cgtgaagggc cagctgtacg
     1141 gtcctacgct caagctcgac ggtacggaca tcaccaccgg cactgctgta accatcgcaa
     1201 ccgacagcac gcgctacgtg ccgctctccg ttgaaggcac gggcagcctg tacctgaccg
     1261 gcatcgccat cgactacgcg gccggctccc ccgccgccac cagccacacg gtcaccgtgg
     1321 gcccgaacgg ccagtaccgg accattcaag ccgctctgga tgctaacgac tcttccgaaa
     1381 ccgaccgact agtgctgaag atcactccgg gcgactatcg cgagaagatc accgtgacca
     1441 agccgggcgt caccttcgcc aacgccgacg tgaccgccaa gcgcgcggtg acgattcgcg
     1501 ccagctacta ttcgtccaac acattcgatg ccgacggcaa gtttgtgccg caggatgaat
     1561 tcgaccttgg caccaacaag tgcgccaccg tgaccattgg cgcgggtgcc acaggtttct
     1621 ccgcctacgg catcaccttc cagaacgatt acaacgtggt ggatcacacg gcggcgggcg
     1681 agcagacgcc ggccgtggcg ctgaacaccc aggcagacaa ggtgtacctg aagaacagcc
     1741 gcatcatcgg ccgtcaggac actctgtacg tgcagggcgc cggcaaccgc gtgtacgtgg
     1801 acggcggcta catcgaaggc acggttgatt tcgtgttcgg cgacgccaac gcctacttcg
     1861 ccggcaccga gctgcacatg gccgcgttcg ccggcaagaa caacggctac ttcaccgccg
     1921 ccaacaccaa gaagtccggc gtgggcctgg tgtttgaccg ctgcaacttg accgttgccg
     1981 cagcatatga cgacgatgcc aagctttcgc tgggccgccc gtggcagacg ttcgcgcagt
     2041 acacgcaggt gcgtaagggc gacggcagca gctatgtgac cggcgtggat ttgggtacga
     2101 agaactcggc gtatacggac acttcctcgg ccgtgaccta cctcgacagc acgatgtcca
     2161 gcaagatcac caagaatcgt tggaatgtgt ggaccagcaa gaaccagagc ggcaaaagcg
     2221 tggacgtgac cttccacgat gatgtgcgat tcgcggaata cgccagccac gatgaaaccg
     2281 gcgcgctgct tgatccggct gattataaga acgtcgtgct cggctcgatg tctgctttgg
     2341 atgaggctca ggtgcaggcg aagcgtgctg aattgctcgc ggcactgggc ttcggctcgg
     2401 cggctggtca gtgggttgtg ccggatggcg cgtggccatt cgcttacgac ccgaactact
     2461 cgaccggcac tgatgtgcag ccgactcagc cgggcggcaa cgcctcgcct aacgcggatt
     2521 cggcggccaa ggccgacacc atgccggaaa ccggctcggc catcattgcg gtggttgtag
     2581 tcgccgtgcc gctgctcgtt gctggcttgg cgctggtagc agtgcgccgc aatcgccagc
     2641 agtgattgaa agcgttgctg cgaaccggac ctggtgacct tgtagacggg ggctatctcc
     2701 gttgaaagag gtacattgat ggctttgcag aaagccaact gctttcaaag gggtacctga
     2761 accacgaaac ctcgagtata agcgttgcaa tgacggcgtt tatactcggg gtttcccata
     2821 ttcagtaccc ctctgaatgc agttagcttg gatcgatgat catatccaag aattcactcg
     2881 tatagtggtg cgtaatgctt acgtatttgg gatagcactt gttgctcgtt gtacatatag
     2941 caatgccctt gcctcctcac ggggaagcaa gggcattacg ctactgctta gtactgctta
     3001 gtattgctca gcactgctca gcgattgctc agcggcaagc gtcacagcac gccctgggca
     3061 accatggcgt tggcgacctt gacgaagcct gcggcgttgg caccgagcat caggtcaccc
     3121 tcgtgaccgt attccttggc ggcggccagg gagttggcga cgatttcttc catgatggac
     3181 ttgagcttgg cgtcgacctc ttcgaaggtc caagacaggc ggtagctgtt ctggctcatc
     3241 tccaggccgg agacggccac gccaccggcg ttggcaccct tggccgggcc gtacagcacg
     3301 ccgttcttct ggtagacctc gatggcctcc ggggtggacg gcatgttcgc accctcgacg
     3361 accacggtca cgccgttgtc cacgagcttc tgtgcggatt cgccatcaac ctcgttctgg
     3421 gtggcgcacg gcagagcgat gtcgcccttg acggtccaca cgcccttgct gccctcgtgg
     3481 tactcggagc ccggaacgcg ctcggcgtat tccttgatgc ggccgcgctc gacttccttg
     3541 atctgcttga cgagtgccac atcgatgccg
//
LOCUS       DAWBMW010000166         3491 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_5557_length_3491_cov_3.525029, whole genome shotgun sequence.
ACCESSION   DAWBMW010000166 DAWBMW010000000
VERSION     DAWBMW010000166.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3491)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3491
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5557_length_3491_cov_3.525029"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          713 a         1066 c         1011 g          701 t
ORIGIN      
        1 ctcgacggta gtgccctcga tggcggcctt agcggcgacg gcgtcctcgc gggtagcgac
       61 ggccttggcc agacgagcgc gcttcatagc gacgatctga gcctcagcgc ccttggtcca
      121 cgcgaaagcg aggccctgcg ggaacaggta gttgcgggcg tagccggact tcacgtcgac
      181 aacgtcaccg gaatggccca ggttggacac ggtcttggtg agaataacct tggtttcagc
      241 cattgttcaa agcctcccta actcagcgac ccgaggtggc gtacggcagc agggccatct
      301 cgcgggcgtt cttgatcgcc ttggacagct cacgctgctc ctgcacggtc acgccggtga
      361 tacgacggga acggatcttg ccgcgatcgg aaatgaactt acgcagcagc gcaacgtcct
      421 tgtaatcgat ctcggtgacc ttggcggcct tcagcgggtt cggcttcttc ttgaacggct
      481 tgaccggcgg ttgcggcctc ttacgtgaca tgatgttctc cttataaatc ttgatattga
      541 ttgctgctgg cagcggggag gcaagtactg atcagaactc cgggtcggaa tcgtcgccgc
      601 cgaaatcgga ggaagcgccg aacgtggaga agctaccggc atcagatgca ccggcattac
      661 tccacgggtc gaccgcagga gcggacgggt tggcgggagc ggcagactgc tgggagtatc
      721 cagcgccacc accctgatag ccaccgttgt aaccgccctg gccaccggca taaccacctt
      781 ggccaccctg gttgccatag ccctgattgg actgctgacc accctggaaa ccaccggaac
      841 gaccctggaa gccgccctga ccggacgact ggcgagtcac ctgtgcggtg gcgtagcgca
      901 gggagggccc gatctcatcg agctgcatct ccacgacggt tcgctgggaa ccatcctgtg
      961 cctgatagga acgctgctgg aggcgaccgg tggcaatcac gcgcatgccc ttcttcaggg
     1021 actgggcgca gtggtcggcc agatcacgcc aagcggagca cctcatgaac agcgcctgac
     1081 catcctcgaa ctggttggtc tgacgattcc aggttcgcgg ggtggaagca atcgtgaagc
     1141 tcgccacggt cgcaccgctg ccaatagtgc gcagctcggg gtccgcagtg aggttgccca
     1201 cgatggtgat gacggtttca cctgccataa cggtccttct tacttacctt gaataccagc
     1261 tgttcagctg actaaacgct tgattcagcc cttacgaaga atcttcgtac ggatcacgga
     1321 ttcgttgagg ttcagaacac ggtcaagctc atcgctggta gcgggctcgg cggtgtactt
     1381 caccacgacg tagttgcctt cggtcttacc ggcaatctca taggcgagct tgcgacggcc
     1441 ccagtaatcg ggctcatcga aagagccgcc ttccttggtg acgagttcca gatactgctc
     1501 ggtcagcttc ttcagaccgc gctcatccag ctcaggatcg gcgatgaaca tcagttcgta
     1561 cttgtgcgca gacatcaccc acctccttcg gactattcgg ccacggacgt tccgtggcag
     1621 gagtgtgtat gcgtgttagc ccatgacgca gcaccaaacc ggcaccgcct cttcgggcaa
     1681 cttggacagt ctatatatga ggcgggacag ggtatgccca tattcttgcc ggtttcgcga
     1741 tgcccaccgt tttggccaag cccacgcagc ccgcggctcc catcactccc ggagcggcac
     1801 cggtagccgc acctggcgcg acctccacca ccatgaatga gcaacagaag aaaatcttca
     1861 tcatcgttgg tgccgtggcc ggcttcctcg tgctgttcgg tatcgtgtat ggcgtgctgt
     1921 cttccgccgt gttcagcgcg aagagcgttg cgaacagcta tgtggccgcc atctcctccg
     1981 gcaactacga caaggccaac accatcgccg atcctcacgt gaatacgaac cagcgcgcac
     2041 tgctgaccag caaggcatcc tcgggcgaga acacgcgcat caccaacgcg cgcatctcgc
     2101 agacctccaa taatgccgat ggcaccgtga gcgcccagct gacgtacacg cttgaaggca
     2161 aggaggtcaa cggcaacttc accatcaaag acaataaggt cagtgtcgag ttctccgagt
     2221 gatttcggca atctcaacac tcttacgaaa taagcacccc gccgtggcgt tcgctgcggc
     2281 ggggtgcttg tacactggtt aaggcgtaaa tcacacagcg actcaaacaa aggatgcgaa
     2341 cgcatgtcgg cgattcttga aggcactccg gataaacgac tcgtattggt cacgggccgc
     2401 gcctaccccg agctagctga gaaggtggcc gaatgcttgg gaaccgaaat cctcgaaacc
     2461 acgtcctatg actttgccaa tggcgagatg tacgtgcgtt tcaccgaacc ggtgcgtggt
     2521 gcggacgtgt tcgtgctgca gtgtcattcg ggcgatatca acaagtggat catggaacag
     2581 ctcatcatga tcgacgcttt gaagcgcgcc tccgccaagt cgattaccgt ggtggcgccc
     2641 ttcctcggct actcgcgcca ggataagaag caccgcggtc gtgagcccat caccgcccgt
     2701 ctgattttcg atctgttcca cgccgccggt gcagaccgca ttatgacggt cgatctgcac
     2761 gccgctcagg agcagggttt cttcgatggt ccggtcgatc atctaaccgc catgccggtg
     2821 ctgctcgatt acgtgcgttc cacccttgat ctgggcaaca ccaccatcgt gtccccggat
     2881 gccggccgta tcaaggtgtc cgaacagtgg gccgccaagc taggcaatct gccgctggca
     2941 ttcatccaca aacagcgtga tattactcgt ccgaaccatg ccgaggcaca tggcattatc
     3001 ggcgatgtcg acggccgcga ctgcgtggtc atcgacgaca tggtcgatac cgccggtact
     3061 atctgcgaag ccgtgcgcac cctgaacgaa tccggcgcca agtcggtgac gctggtcgcc
     3121 acccatggcc tgctctccgg cccggccgtg gagcgcctgg ccaactgtgg cgcgcatgaa
     3181 atcgtgttta tggacaccgt gccgattccc gaagagaagc gcctgccgaa tatgaccgta
     3241 ctgtcttgcg caccgctgct ggccgcgggt attcgctccg tgttcgagga aggttctgtg
     3301 gcgattctgc tcgacggcct gcccgaagac atgcgtccgc gcaatatcta tgcgtgattg
     3361 atacgccgaa aatccttacg aaaaaacgcc gcttgactat agctgggcgt acgtaagaca
     3421 gtattgcgcc gagatcgacg aagcggcacg aaaccgcatg gagctgatcg tgcggtcact
     3481 ggcagagcaa a
//
LOCUS       DAWBMW010000167         3476 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_5590_length_3476_cov_1.881614, whole genome shotgun sequence.
ACCESSION   DAWBMW010000167 DAWBMW010000000
VERSION     DAWBMW010000167.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5590_length_3476_cov_1.881614"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    389..398
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1616..1711
                     /estimated_length=96
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3006..3015
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          615 a         1062 c         1014 g          669 t
ORIGIN      
        1 cggcatcacc gctcaagcca atcaggtcat ggccgatctt gaccttgcag cgttcgtcga
       61 acggcatccg atgagcctgt ccggcgggca gaagcagcgg gttgccatcg gctcggcatt
      121 gatgtgcggc aaggacctca tcatccttga cgagcccacc agcggactcg accgctacca
      181 catggagcag gtcggcgaac tgctgcggca actggcgagc caaggcaagg cgattctcgt
      241 ggtcacccac gatgaggagc tcgccgccgg ctggtgcgac cgcattgtca atcttggcgc
      301 cgacgattcc gggaacaacg gctcgcatgc cagtaaccca gatgccagca gccccaaccc
      361 taatgcctct accaatttcg ccaccgcann nnnnnnnnca gcaacaccac ggattccacg
      421 gccttcgaca tcaccgactg gctcggtgaa tgggaaagct tcgagcatta cattgattcg
      481 gatgacgccg ccatccagca gacctgggaa gccgccgagc aggcggtgct cgccaacccg
      541 aagatggccc cgatggccgc gcgcggcatc aggacgttct ggtcgatggc ttgctccacc
      601 accagcccgg agaacatcat ccatatcggc tactggcgtg tgaacgagcc ggccgccgaa
      661 tccggctcca cggacgatgc cgcgctcgcc atcgaatggt tcgccgagga cgacaccagc
      721 ctcgacacct acgaatacac catcgaccat gtcatcgagc acggtctcga aggctccccc
      781 accttcgtgt tccacaccac cgatccggcc gccgaagaca gcccgttccg ctggctactt
      841 gccatcaatc cgctgccttc gcgcaaggcg ttcgccgagg gcgggctgct cagccacctg
      901 catttccagt acgccaacga tctgcacacg ctcgtcgcca cggatgaggc caccggcgtc
      961 gagaccttgc gcaatccgcg ttggtacgcc accatgtgcg ccaacgaggg aaccgtcgag
     1021 gaccgttgcg ccatcatccg cgcgttgcac cacttgcagt agacctggca acatacggca
     1081 gtgacggctc cccactagtg aaactagcat cgcttcagta gccgtgcgag tgcagatact
     1141 cgtcatcgaa gccgaaaaaa tggccgatct cgtgcaacac ggttttgcgg atccgttggc
     1201 gcagctcggc ctgcgttgag catacgcgtt catgcggccc cttgaacagg gtgatgacgt
     1261 ccggcatcac gccgctgtag cccgtagttc gcttcgtgac gggtatgccc tcatacagtc
     1321 cgagcagctc accgcacgga ttgctcattg tagccagttc gcgctcattg ggttcgtccg
     1381 caacgacgat gccgacattc tccagcaccc gcgcaaaccg ttcagacaaa tcatccaaca
     1441 cctgttcaat ggcccgttca aactcatcgt cactgatgtg cattcgctct ccacacgcga
     1501 aatgccttgg caccaagtat gtaccaaggc gctgaaatct cagactagca ggctcagctg
     1561 ttgcggttga tctcgatgtc ggtgacggtg aggttggcct cgttgatcag gttgannnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncttggtggc gcagtacacg ctttcgttcg
     1741 cgttgccgcg tgtggcggcg gtgctcataa tctgggcgat tttcacgttc tgctcgccgc
     1801 aggccttgag ggtttccacc gtccacagaa tcatgccttt gagtcccttg agacatttgt
     1861 cgacgtcggc ggcctcgtag gcggtgggca ccttgaacga aggctggccg gcgttgttga
     1921 tgagcaaatc gatgtggccg agcgtggcga tatccgccac ggattccttc acgaacgctt
     1981 cgtcggtgat gtcgccgacg taggcgcggt aggagtcatc gggccactgc gcggtcagtt
     2041 cggcctgacg ttcggcattg aagtcgacac cggccaccag ccagccgttg gcgatgagct
     2101 ggctggcgag ctcgtagccc aagcccagcg cgctgccggt gacgatggcg attttcttac
     2161 ctgttgtggt tggcatggca gacctctcct actactattc ttcggctacg agtttactac
     2221 tattcttcag ctacgagttc gagatacgaa tcattccaga ggtcttcgtc gccgtcgggc
     2281 atgaggagca cgcgctcggg gttgagcgcc tcgacggcgc cctcatcgtg ggtgacgagc
     2341 acgatggcgc cctcatactt ggcaatggcc ttcagaatct cctcacggga agcggggtcg
     2401 aggttgttgg tgggctcgtc gagcagcagc acgttggctc gcgaggtgac cagcgtggcc
     2461 agagccagac gggtcttctc accgccggaa agcacgtgag ccggtttcat cgcgtcgtca
     2521 ccggagaaca ggaacgagcc gagaatcgaa cgggcctgcg tgttatcgag ctcgggcgcc
     2581 acatgctgca ggttctccaa cacggtggcg ttcagatcca aggtgtcgtg ctcctgagcg
     2641 aagtagccga tcttgcagcc gtgaccgtat tccacggaac cggtgtccgg ctcctcgatg
     2701 tgggccagca ggcgcagcgt ggtggtcttg ccggcaccgt tgtagccgag gatcaccaca
     2761 cgcgagccct tgtcgatggc caggttcacg ccggcgaaca cgatgttgga accgtaggcc
     2821 ttggaaatat ccttggccat gatcggcgtg cggccgcagg gcgcgggctc ggggaaacgg
     2881 atgtccgcca ccttctcggc cttctgcgcc tcggaggtgt tttccagcag cttttcggca
     2941 cggcgcatca tgttctgcgc cgccacggcc ttggtggcct tggcgtgcag tcgaatgcct
     3001 tgcttnnnnn nnnnncggcc ttcttctcgg ccacttcgcg ttcgcggcgg cggcgctcct
     3061 cgtccaccac gcgctggtgc aggtaggcct tccagccaag cgaatacatg tcgatctggc
     3121 cgagctgcgc gtccagatgc cagaccttgt tcaccacttc gtccagcagt tcggtggagt
     3181 gggagatgac caggaagccg ccctcgtact tcttcaagta gccgcgcagc cattcgatgg
     3241 aatcggcatc caagtggttg gtcggctcgt cgaggatcag ggtgtcggca ttggagaaca
     3301 ggatgcgcgc cagctcgatg cggcggcgct ggccaccgga gagcgtgccg agctgctgct
     3361 ccatgacctc ctgcggcagg ccgagcgagg cggccatgga aatcgcctcg gactgggccg
     3421 cgtagccgcc ggccttgtcg aagtcctgca tggccttgtc gtaccgggtc atggcc
//
LOCUS       DAWBMW010000168         3472 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_5598_length_3472_cov_3.295288, whole genome shotgun sequence.
ACCESSION   DAWBMW010000168 DAWBMW010000000
VERSION     DAWBMW010000168.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3472)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3472
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5598_length_3472_cov_3.295288"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3124..3133
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          722 a         1119 c         1002 g          619 t
ORIGIN      
        1 acggtggtca cggtcacgaa cgcccaccac aacgccgtgc cgaagtcggt gatcgtggat
       61 ccggacgccc cgcgctccac ggaatacacg gccagcgcgc ccacgtacat gagcattccc
      121 accgcgccga ccgcgtatac ggtgatgcgc ccgcgcaccg ccatgccgga ggtgcggttc
      181 agcacgtgca gcacggtcag caaacgcaac agtcgcaagg gccggagcat cggcagcgcg
      241 atggtcagca gcgtgaacag gttgtgcttg aaccaccgcc agcgatcgtc ggccaaccac
      301 agcgagacca cataatcgac gacgaacacc acccagatca cgttgatgac ggtttcacac
      361 agcgcgatat gcgtgcggga gagaatttcc cacgaataca ccaccagaaa caccacggac
      421 agcaccatca gcggccagtc cgtccacttc ttccacgtct tcagtcccat attccgattg
      481 tagtggcgcg cccgctgcgc cactgcggag catgactccg gacgtcagtg ttgtatacgt
      541 tcgtatacat ttcacgttgt gtacgaacgt atacaacggt atctttcatc tcgcatttcc
      601 cttatgacta tcaccacagt gatataatca agaatatgta aggagagaga gaaggagccg
      661 gccaatggag accgcatcga tcgaactgtg gcatggctct tcgcatgtca tcaagcatcc
      721 tgaatacggg ctggtcaagc cgaacaacga ttatggccgc ggattctatt gcacgcgcag
      781 cgtcgagctg gccaaggaat gggcgtgtgc gggactggat gacggtttcg cgaatcggta
      841 cacgttgacc acggacgggc tcctcttcct ggacctgtcc cagccgccct acaccatcct
      901 gaactggctg gccatgctgg tcgaaaaccg tcgtttccag ccgaccaccg ccgtcgccgc
      961 gcaaggcatc gaatatctga aacaagtgtt tctgccggat gccagtcagg ctgacgtcat
     1021 cgtcggatat cgtgccgacg attcgtattt ctcgttcgcc cgcgcttttg tcaataacgc
     1081 gatttccatc gaccaactcg ctgaggcgat gcggttgggc gagctcggca agcagatcgt
     1141 actcgccagt cctcaggcat tcgcggcact gcggttccaa ggctgcgaaa tcgcccgcgc
     1201 ttcgacctac cacggattgc gtaacgagcg cgaccgcaca gctcgcgaag cctaccgccg
     1261 caaaagtgca gaagccgatc tcgacggcct gttcatgcgc gacatcatcc gcgagaaagt
     1321 gaccaacgat gatccccgcc tatgatgaga catacttgcc ggacgccatg gccaacttgg
     1381 ccggcgcctt cgattatgcg ctgaacgatt gcgggttgcc ggtcggccta ttcatggact
     1441 tcttcgccac ctccgaggcg gccgcgcttt tcgggcgagg cacgcccaaa tacgtgtgtg
     1501 gcatgtccgg cgaggagctg gtggccgaaa tcatgcggca aaccggttgg gcgcgcattc
     1561 tcgatatgcc gccagccgca ctgcgcgccg gctcaacacc cgaatactgg gccggatggg
     1621 tactcgcata ctgccaatgg gccaggggag tgcggttctc ggacattctc gatgtgctgt
     1681 cgctcgacga tatcgtgcga ctgcacccga ctctgcacga agccgacgaa agccgattcg
     1741 tcgacgtgta cgacgagcgg gccgcccata atcgcaccga gggcgactcc cggctgcaca
     1801 cgatccgcgt gcgcgccgga ctcagccaat ccgggctggc ccgccgctcc ggcgttaccc
     1861 tgcgctccat ccaaatgtac gagcagcgcc gcaaagacct tggcagagcc gccgtctcca
     1921 ccgtgctcgc tttggcccgc acgcttggct gccgcatcga agatctgttg gagccgtagg
     1981 gttaacagcg ccatattttt tttactcccg accgattgtg cgtgcgaaac gacaggttgt
     2041 gtaatacggg cggcgcggcc cgggcgaacc tgattcaatc aaactagcga tggatcgtgc
     2101 gttcgcggac tgccacatcg gcctcggccg agtcgggcac tgcgccgagt tggcactgca
     2161 cagacgcggt gcctgcggcg tgacgcaacg gattcgtcag acagccgtac aacagtgcaa
     2221 gggagaacga tatggaactt gaagaactat cggtcaccga gaaagccgcg atgctctcag
     2281 gtggctccga atgggattcc cgcggcaacg agaaggccgg catccccaac ttcgtgatga
     2341 gcgacggccc ccacggtgtg cgccgccagc tcggcgaagg cgaccatctg ggtctggggt
     2401 catccaagcc cgccacctgc ttcccgaccg ccggcaccgt tgccagctcc tgggatccgg
     2461 cgctcgccga agaaatgggc aaggcacttg gcaacgaagc gcacgatctg gacgtgaatg
     2521 tgctgcttgg cccaggcctg aacatcaagc gcaacccgct gtgcggccgc aattttgagt
     2581 actattccga agaccctatc gtggccggtc gtatggcagc cggtctgatt cgcggcattc
     2641 agtccaacgg tgtttccgcc tgcccgaagc atttcgcggt caacagccag gagctgcgcc
     2701 gtcaagcctc caactccgtg gtcgatgagc gcacgatgcg cgagatctac ctgaccgcgt
     2761 ttgaaatcgc cgtgcgtgag gccgcgccga tgaccatcat gacttcgtac aacgagatca
     2821 atggcgttta cgcgcacgag aacaagcacc tgctgcagga catcttgcgc aatgagtggg
     2881 gtttcgatgg catggtcgtc tccgactggg gcggctccaa ctccgccgtg gccgcggtca
     2941 aggccggtgg ctcgctcgaa atgccgtcgc ccggctacac ctccgtgcgc gaactcgaag
     3001 gcgcggtcaa ggccggcacc ttgtccgagg ccgacctcaa cgctcgtgcc gccgaagtcg
     3061 ccaagattgc acacttgacc aaaaccgaag gcgttggccg caacgacttg ctcaaagatg
     3121 ccgnnnnnnn nnnccgctgc gcaccacgaa atcgctcgca aggtggccga gaactcgtcc
     3181 gtgctgctca agaacgcttc cgccacgctg ccgctgcagt ccggcactcg cgtcgccgta
     3241 atcggcgaca tgtccaaaac cccgcgattc caaggctccg gctctcccaa ggtgaatgcc
     3301 actcgcgaag agaacatcct tgatgaactc aagaacgccg cagcaaccac cggcgtgacc
     3361 gtggccggct acgcgcaagg ctacgaccgc caaggagcca agaaccagac gctcatcgac
     3421 gaagccacaa cgctcgccgc cgccgacacc actgatgcgg tgatcgtcgt cg
//
LOCUS       DAWBMW010000169         3429 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_5670_length_3429_cov_2.882336, whole genome shotgun sequence.
ACCESSION   DAWBMW010000169 DAWBMW010000000
VERSION     DAWBMW010000169.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3429)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3429
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5670_length_3429_cov_2.882336"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2108..2117
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          750 a         1036 c         1001 g          632 t
ORIGIN      
        1 gttcgccgac gaatatccga ttcaggccgt agtcaacaag caggatttgc tcgacgccat
       61 cagccgtgtg gctttggtcg ccgaacgcaa tgctccgatt cgcatgacct tcaccggtca
      121 ggaagtggcg ctgtccgccg gatcggtcga cgaggctcag gccaacgaaa cactggacat
      181 cgacatggat ggcgaagaca tcaccgtggc attcaacccg tcctacctga aggagggtct
      241 gtccgccgta accgaaccat tcgtgcgcat caaaatgacg accccggtca agccggtcga
      301 gttcaatggc cagcaagaag ctgattccga cgagtccatg gactaccgat acctgctcgt
      361 gccgatgcgc tttaacaact gaaatccctg aatttccacc gactcgctaa tgcacatctc
      421 ccgtctcgca ctcgaccact accgctcatg gagtcaggta gtggtcgatt tcgtaccagg
      481 cgtgaatatc ctgttcggca agaacggtct gggcaaaacc aatctggtgg aagccgtcga
      541 agtgctgtcc accggatcga gccatcgcac gtccagcacg ttgccgctga tcgaacgcgg
      601 gcagaccacg gccaccatcc gcgcgaacgt cgctgatgat gccggtcaaa ccactacgta
      661 tgaagcgtca attcatgcgc gtggtgccaa ccgagcccgc atcaactcgg ggtcgtcgct
      721 gtatctgcgc gatatcatcg gcaagattcc cagcgtttcc ttcactcccg aagatcagcg
      781 gttggtgtcc ggcgatccgg gagcacgccg caccatgatg aatcaggctg cggctctgct
      841 cgagcccgga tacatgcaga cgttgcaaca gttcacgcgc atcgccaaac agcgtgccac
      901 attgttgaag caattgaatg ccaatgtcaa caatggccag ccgatggatg cggttctgag
      961 cggtttggaa atctggaccg gccagttcat cgaagccggt gtggcgttga cccgtatgcg
     1021 cgctcatgtg atcggtctgc ttgccgagcc atttgccgcg atttacgccg acttggccgg
     1081 tgccggcgag caggtgacgc tcacttatgc gccttcattc gacgaagtgc tgatgttcga
     1141 cgatccgcat ccgcagatta gcgagcattt ccaacgcatc taccccggtg aagtggctcg
     1201 cggcgtgaac cttatcggtc cgcagcgcga tgacatgaac cttgagttgg gcggtattcc
     1261 cgcccgcgag ttcgcttcga acggcgagat gtggacgatg gcgttggcct tgaaaatggc
     1321 attgttcgaa atagtgcgtg accggttggg attgcagccg attgtgattc tcgatgatgt
     1381 gttcgcccag cttgacgaca gccggcgcac gcagattctt gatttcgcca gaaaacagga
     1441 tcaggtgctg attaccgtgg ccgctgaagg cgatgtgccc gactatgaat cggcccatcg
     1501 catcgacgtg gccgcactgg acgaaccagc ttccgtgata ttgccaactc tgggcaattt
     1561 gtccaattcg cgggaaaatg agagtttggg tgggtatgac gaaaaaaccg cggtggacag
     1621 cgcgtccgta gaacggaaag tgtcatgaac ccgccgatcg cccgccaact caacctggac
     1681 gagaccaaac tgccggccga aatattcgag cggctctccc gtcgcggggc gaccttgaag
     1741 gactacagac gccgccgtga agaggccatc gaaaacttcg gcaaaccggg gcgcgatccg
     1801 gctgaactgg gcagcgtgat gaccaccatc gccggcaacg gcgtctggac ggcgaatatg
     1861 aagcttgcgc aattgcgcaa ccattgggac caagtggtgg gtcaggccat cgccagccat
     1921 tcggcagtcg ccgatttcac tgacggcgta ctgaccatcc gcgccgaatc cacggtctgg
     1981 gccacgcagc tgacctacct tatcccccaa ctcaccgata ccatccgtcg caatctcaaa
     2041 ggattgacca tcaccgagat tcgcgtgacc ggcccggccg ccggatacac gcgcaaatgg
     2101 gctcggcnnn nnnnnnngta gccaagatac caaacaaatt cgcagaagcc tcgctatggc
     2161 gctcgtcggg ttagacgagt aaaatcacat gcagtatggt caaatggcta gaagagccct
     2221 ttacgggcct cataagccat atcgcctccg tgaatgacct tcatgacgga ggttgaccct
     2281 gtagtcacgg aaggaagcct gttggcagac gaaaccagca aagaggagca gctgaaggcg
     2341 gctgcggaac gcatcaacaa tgcggaattg accgaaggcg aacttgacga atccatggcc
     2401 cccgagcatt atgaggcctc cgatctgagg gtgctcgaag gacttgaggc cgtgcgtatc
     2461 cgccccggta tgtacatcgg ttccaccggc ccgcgcggtt tgcaccacct ggtctatgag
     2521 atcgtcgaca actcggtcga cgaggcgctg gccggatacg ccagccatat cgaggtcacc
     2581 attctgccgg acaacggcat ccgtgtggtg gacgatggtc gaggtatccc ggtcgatgag
     2641 gtgcccggcg agggcgtctc tggtgtggag accgtgatga ccaagctgca cgccggcggc
     2701 aagttcggcg gcggcggtta tgcagtgtcc ggcggtctgc acggcgtggg catctccgtg
     2761 gtcaacgcac tctccacccg cgtggacatc gaagtgcgcc gtcagggctt ccactggacc
     2821 cagacgtaca tcgaccagca ccccaccgcg cccttgaagc agggcgagcc gatgaccgaa
     2881 ggcgagtcca ccggtacgtc tgtgactttc tgggccgacc cgaagatttt cgagaccacc
     2941 atctacgact tcgagacgct gcgttcccgc ttccagcaga tggccttcct gaacaagggc
     3001 ctcaagatca gcctgactga cgagcgtgag aacgaccagg ccggcgacga ggtggccggc
     3061 gacgccgcgg acgagcccgg cgcaaagcac cagaccgtca cctaccagta cctcaacggc
     3121 atcaaggact acgtcgacta tctggtcaag gtgcgcaagg ccacgccggt ggaagaggac
     3181 gtcatcagct tcgaggccga agacctgaag ctcggcatct ccgccgaact cgccatgcag
     3241 tggaccactg cctactccga ggccgtgcac accttcgcga acacgatctc caccaccgag
     3301 ggcggtaccc atgaggaggg cttccgcgcc gcgctgacct cgctggtcaa ccgttacgcg
     3361 cgagacaagg gcatcctcaa ggacaaggac gagaacctct ccggcgacga cgtgcgtgaa
     3421 ggcctgacc
//
LOCUS       DAWBMW010000170         3395 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_5751_length_3395_cov_2.162575, whole genome shotgun sequence.
ACCESSION   DAWBMW010000170 DAWBMW010000000
VERSION     DAWBMW010000170.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5751_length_3395_cov_2.162575"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    772..781
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          643 a          999 c         1033 g          710 t
ORIGIN      
        1 agccgatgat attgtcaccg ggcaccggca tgcagcaacg ggccagcttg atccacacat
       61 cgcccacgcc cttgacggac acgccggtgg agctgctgga ggccttgcgt tccatgggct
      121 tcagcggcag gacttcctgc tcgacttcct catcgacctc gtcggcaccc gcatccttga
      181 ccagatgcga gatgacgttc tgcgtggaga cctgtccgtc accaatggcg gcgaacacgg
      241 cgtccgcgtt cgggaagtta aggtcgtcgg ccacacccac cagtgcttcg ggcgtaagca
      301 atgtgttgac cggcagattg cgcttgcgca tcgcgcgggt cagctcgtcg cggccctcct
      361 cgatggcttc agtacgacgc tccttggaga accactgacg aatcttgttg cgggccttcg
      421 gactcttgac gaagctcagc caatcgcgtg acgggccggc tgtatcggat ttggacgtca
      481 gaatttcgac ggtatcgcca ttatcgagcg tggtgtcgag cggcaccaaa cgaccgttga
      541 cgcgcgcgcc catcgtacgg tggcccactt cggtgtgcac ggcgtacgcg aagtcgaccg
      601 gcgtggcgtg agcgggcagg gatacgattt tgcctttcgg cgtgaacacg tagacttcgg
      661 acgagccaag gtcttccttg agcgagccca agaattcgtt ggaatcaggg gtttcgctgg
      721 tccagtcggc gagctgctga atccacttga ggttatcgac ctcggaaagc tnnnnnnnnn
      781 ntgttttcat cacgctttcg atcggacttg tccggcgagc tcagggcgcg gccggcctgg
      841 ccgttttcct tgtatttcca gtgcgcggcg atgccgaatt cggcgcggcg gtgcatgtcc
      901 caagtgcgaa tctggatttc gaccggcttg ccaccggggc ccaccaccgt ggtgtgcagc
      961 gactggtaca tgttgagctt cggcatggcg atgtagtcct tgaaccggcc gggaaccggg
     1021 ttccaccgcg catgcacggc accaagcgcc gcatagcagt cctgaatggt gtcgacgatg
     1081 atgcgcacac cgaccaaatc gtagatgttc gcgaaatcat ggccgcgcac gatcatcttc
     1141 tggtagatgg agaagtaatc cttcgggcgt ccggtgacgt atgccttgat gttctgctcg
     1201 tcgagatcct cgttgatctc cgccagaatc tgcttcaggt acacatcgcg ctggccggct
     1261 cggcgagcca ccagcaccac gatctcgttg tagatcttcg ggtagagcac cttgaagctc
     1321 agttcctcga gctccgtctt gatggcgttc ataccgagtc ggttggccaa aggtgcgtac
     1381 acgtcaaggg tttcacgcgc cttcttctgg gcggacgtgg tcttcacata gcgccaggtg
     1441 cgagcgttgt gtacgcggtc ggcgagcttg accaccaagg tacgcacgtc gcggctcatg
     1501 gccaccacga gtttgcgaat ggtttcggct tgggcggaat cacccacttc aagctgactc
     1561 agcttggtaa cgccatcgac cagaccggcc acggtgtcgc cgaattcggc gcggcattga
     1621 tccaacgtat agtcggtgtc ttccaccgtg tcgtgcaaca ggccggctgc caccacgatc
     1681 gggcccatgc ccagatccgc gagaatctgg gaaacggcca gcgggtggat gatgtacggc
     1741 tcgccagact tgcggcgctg ggcggagtgc tggatgacgg cacgccggta ggcacggtca
     1801 agaatggaca aatcctcatc ggggtgatgg gacttgcaca ccttcttgat tggatccagc
     1861 ggattgagcg gatcggcact gatctcacaa cccaacttac gggcggcgta ctgctcgcca
     1921 tcaatctctg ccatgccagt cacctccctc gtcatgaagt tcattgtagc cgcgaaccgg
     1981 agccttgtgc attcacctgt tcgccatcat tggttccaca accgttgttg ccgtcatcac
     2041 cttagtcatt caaccggcct aatccggtca ggacgccact cccgtcgaac cgaacccgcg
     2101 ttcggcgcga tcggatccgg gcagtgtctc ggccgggatg aaccgagcct caacataccg
     2161 ctgaatcacc aattgagcga tgcgatcgcc cggatggaag acggcggtgt gcttcggatc
     2221 gaggttgatc agaggcacct tgatctcgcc acggtagccg gcatcaacgg tgccaggcgc
     2281 gttgagcacg gtgacgccct gcttggcggc cagcccggaa cgcggatgca ccaacgccac
     2341 gtagcccgca ggtagggcga tggccacgcc ggtgggaacc agtgcccgtt caaaaggctt
     2401 cagcgtcaca tcgacggtgg tgatgaggtc ggcaccggca tcgcccgcgt gggcatagtg
     2461 cagctgggcc ggatgttcgg ggtcgaggct cttgaccagg acctcggtgg attcgggctc
     2521 gttgtaggtc tcgtcgaacg ccatcaggcg gcgcactcct cacacaccgg ctggccatcc
     2581 tcgtccgtgt aggcgagctg gctgcgatgc ttgaccagga agcactgcga gcagatgaac
     2641 tcgtcaccct gcatggggat gacggtcacc gaggaatcct cgttgctcaa atcggcgccc
     2701 ggcagttcgt agtcttcggc gatggcgttc tcatcgtcgt cgatgtcgct cgaggcgttc
     2761 tgcgaggact tccccagcgc ctgcagcgat tcctcgtcct catccttgtt gcggggggaa
     2821 tcataatcct gagccatcat ggtcctttcg tcgtctttgg ttcgtaagtc gatatatata
     2881 gcacggtcag cgccgtactc gcccatagga tacacgattt gaaatactcg taaagttcga
     2941 cacgtggggc atacttaaaa gaagtaatgt ctttgcgacg gaaagtggtg cgaatatgcc
     3001 agatgatcgg cttgaaaaag cccgtttcgt gcgcgtgagc aagacggggg acctcgtatt
     3061 ctcccttggc ggcagggaaa tggctgtgag cgtggacgac acgctcgaac gggcgattct
     3121 tgaggccaag caggtcagaa gcgagatgcg acaggctccc cagccgcatc agcagtccac
     3181 tttgccgatt tcccagatac agtcgctgat tcgcgccggt gccgatccgg cccgtgtcgc
     3241 ggagaaatat catctgagcg aaatgctggt acgtcgtttc tccatggctg tggagaccga
     3301 gaaacagtat gccatcgaac agttccttgc cgtggcggca cccaaagaca gcagggtgcg
     3361 caccatcagc gaactggtgg aacgcacgct ggcct
//
LOCUS       DAWBMW010000171         3389 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_5764_length_3389_cov_2.547690, whole genome shotgun sequence.
ACCESSION   DAWBMW010000171 DAWBMW010000000
VERSION     DAWBMW010000171.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 3389)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3389)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3389
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5764_length_3389_cov_2.547690"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          737 a         1025 c          925 g          702 t
ORIGIN      
        1 ttccttcatc gagttgccgg aatcccagcc ccagaacacg agttcggcct tgtcagcgtc
       61 acccttgttg gcgccgttgt cgttgctgct gccgcaggcg gcgagaccga cgagcgtggc
      121 cacggctgcc gttgccgcga tgaccttctt ggtgttgcgc atgttatctc cttctggacc
      181 attggagttg acttgcgggt ggttggcttt cttccgactg gcttccggtt ttcgtccggt
      241 tacttctcgg ttatcttccg gctgtctttg gctgccttcc gattgttgac gactgccttc
      301 cgattgccaa aaacgaatcg actgctgcgt tggatttcaa ttcgtttggc cgcttgcgaa
      361 gtctcctctg gccacctttc cttggggtgt tttctttggt acacccgtgt accaatacct
      421 aagatgatac acctgtgtac caatttgtca aatcacccga cacgcctgat ttttttcgcc
      481 ttttccatgc tttccacgtg agcaaatctc cagtcgaaca gcgacttgtc tcagaggctc
      541 ttcatcccct cccccaacgt gagtaggtcc cgactctcag cacaccacca aataacgcgc
      601 agcatgctcg tgagtcccca acacccacac accacggcgc gattccacca ctcagccgcc
      661 tcaccagtgc ccacatttgt agatattcaa cgccatatga caagaatctg actagtatga
      721 acaggcaccc cccatagctg tattgagagg aaaagcacat ggcgggcctg tttcaagctg
      781 cttccaagca gcgcaatccc ttgggaacaa ccatacgtac tcacggatcg ggcaaatata
      841 cgttcggtct ggccttaagc gccggtcaat atccgcggtc tgcttgcctt ttcctatcag
      901 ctcctcgcgg tatgcggcat cttcctgacc aatgtcatca actacatcat cgcctcctac
      961 ggttccagaa gctgggatgt caacgccggt tggcgttgga tgctgaggct gggtgcgatt
     1021 ccggccgccg cgttcctgct ctccatggtg cgagcaccgg aaagcccacg attcctcatt
     1081 caagccggca aaaccgaaga gggattcgcg gtgctcgagc acatcatcgg caccgaacag
     1141 gcacgccttc gcactgacga catccatgca tcggtcaaac tcgaaaccga aatgtcgcat
     1201 gaatttcacg acctgttccg cccagggctg cagaaggcgc tcatcatcgg cactttgatc
     1261 aaggcgtgaa tgctgcgttg cgttactgca ttgccatgtt gccatgttgc cgcgttattg
     1321 catcgctacg ttgtcacgtt gtcgcgttat tgcatcgcta cgttgccacg ttgccacatc
     1381 acattgcatt gccgcattgc tgcgttgccg catcacatat cgcaacacaa acgcagcaac
     1441 gcaatgacga ccggagatgg catcgccgcc acccgcgagg aaccgcagtg caacgcggta
     1501 acgtaacgca atgcgatgta attcccgcgt agttacaggc agttacagat tgagtaccgc
     1561 atcagcaatg cgcatggcgg attcgcggtg ggagaccagc accacagccg cgccgcgctc
     1621 cttgtctgca tgctgctcac ccacggccat agcgtgaatg gaacgtagaa taatagcctc
     1681 gttcaaagca tccaaacggc tggtcggctc gtcgaaaagc accagatcag cattgcgcag
     1741 gaacacacga gccaaaccga tacgctggcg ttcaccttcg gatagacggt cgcccaactc
     1801 gcctacctga gtatcaaggc catcgggcag ggaatcaatg agatcaagca cggaggcctt
     1861 ggcgagagcc tgacgcaaac gagcgtcgag accgtcagat gctgccacct gaacggcatc
     1921 gccctctgcc gattgcgccg ccaaccgctg ctgtcgggaa tcaacggtct tggcacccac
     1981 ttccccattg cctgcaacat ttgcagcttc aacctcggac tcaggcaaag caatcagcag
     2041 attgtctcgg atagagccat caaacaggtg ggtttcctgg ctcatcatgg cctgaatacg
     2101 tcggcgagca tgcacatcga cctcgggcaa cggcgtgccg gacaacgtca cctgaccgcg
     2161 ctgcggatcc cagtagcgca gcagcagctt gagcatggtg gacttgcctc ggccggacgg
     2221 cccctggata cccagcacgc catgctgcgg cacgtcaagc gagaagtcgg acaggacttc
     2281 gcgctccccc gcatatgcaa aggtgacgtc atcaagacgc atgcccatat actcctgcgg
     2341 cacaacagtg ccgttctcgg tcacagcggg cacctcgtcc atcagtccga acagtcggct
     2401 agcggacgcg aaggtctgag tcaggttagc gggcaaggcg gcgagcgcca aagtcggtcc
     2461 gaacgagccg gcgaacagca cgaaggcggc aatcagagcc ggaatgctct gcgggttcgc
     2521 gccggcacag gcaatagcca cggcgaccga agccgctgtg agcacagcca ccaagagtcc
     2581 gctgattccg gtgaaacggc cgttgacctt gctgagtttg gcgtgatcct tccacaacgc
     2641 atgggtacgg tcctcgatgg cctgcgcacg ttcctcgccg cgaccaaatc ggatgatttt
     2701 gtccaggccg cgaatgtcat cgagtatcac atagtcgagt tctccggcag cgcaacggat
     2761 ggcagggcca aggttacgta cgccggtggc aaagaatcgc ggcacaagga tgccgataac
     2821 caggtgggcg gcaatcaacg ccagcgccat ccatgggctt agggtggcgg ccacaatcgt
     2881 gtagatgatg gtggaggtta ccgcgatggc caccggactg atggtgtggg caaagaagat
     2941 ctcaagcagt tccacatcgg tggtcagcat ggcgataagg ttgcccttac ctttgcccgc
     3001 gagcttggcc ggcgccagtc tgcgcaacgg aagagtgcga gcagatggaa tgccacattg
     3061 tggttcagat attgttcaat gtatcgggtg atgccacgca gaagagcaca gataatcatc
     3121 atgatgacgg cgggcgcaac acccatgccc cacaccggat taccggtaag tgcaaatgcc
     3181 gcgataatgc cgaacacggg caggaaagtg gccgaaagat ggccgatggt gccggcggtg
     3241 gaggccgctg ccatcagtcc tgccagaggt cgggcttcct taagcagtcg cctgatcact
     3301 tggaatgtgc tcattttgga agacgaatcg ctatttgcaa caccagccgg agtctcagcc
     3361 gaggcctgct cactcccgcc aacggaagc
//
LOCUS       DAWBMW010000172         3374 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_5789_length_3374_cov_2.156372, whole genome shotgun sequence.
ACCESSION   DAWBMW010000172 DAWBMW010000000
VERSION     DAWBMW010000172.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5789_length_3374_cov_2.156372"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2023..2032
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          607 a          960 c         1078 g          719 t
ORIGIN      
        1 agcgtacaga gcagccaggg agacgcccag gatggcgttg gcgcccagct tgcccttgtt
       61 cggggtgccg tcgagctcga tcatcaggtc gtccagagcg cgctggtcag cagcgtccat
      121 gccgatgacc ttcggagcga tgatctcgtt cacggccttg accgcgttaa gaacaccctt
      181 gccgccgtag acggacttgt cgccatcgcg acgctcccaa gcctcggcct caccggtgga
      241 ggcgccggac gggaccaggc ccaggccctg ggcgccgtct tcggtgtcca gaacgacctg
      301 aacggtcggg ttgccgcggg gatcgagaat ctgacgcgcg taaacgcttt caattactgc
      361 cacaactact ccttaaaacg gttgtgtatc ttgaatgaca ttcttaagag tagtgcgatt
      421 tgtggacgtt gtgaacaaca cgcccaaaat ttagcgttag cgctcacaat gccagtgtgc
      481 cgcctgattg caaggcagta cggacgaaat cgctcagtca acgtccttgc cattgaggta
      541 gtcgatttca ctgtcggaca atttcagttc gctggctttg aacgagtcct gaatctcctg
      601 cgggtttctg gccgagggaa tcgtgaacag atattggtat tgcgcaagtt cccacgcgag
      661 cgtgacgcgc tggtaggagc agtcgtgaat cttggcgact tcgcggaacc ggtcgaacag
      721 atgctcgttg aacgggtcga ggaatccgcc gagcggcgac cagcagacga aagccaaacc
      781 gagcttttcg caggtttcca acgtgtgttc agggtcgcgg tggaccgggg agaactggtt
      841 ctggacggcc atgagacgat cgcccaaaat attgcgggcg gtttcgatgt ccgcgttgtc
      901 gattctggag atgccgacag ctttggccac accttcgtcc acgagctgct tgagtgcgcc
      961 catctggtct tcgtacggca cctgcggatc attgcagtgc gagtagagca gatcgagcgt
     1021 atcgacaccg aggcggcggg ctgattcctt ggcattggcg atcatggttt caggtcgggc
     1081 gtcggcctgc cagccgtagt tgccgttgcc gtcaagcgtg cgcagccagc cggttttggt
     1141 ggctacgagc acctgatctt tggggccatg ccacgagtgc agagcatcgc ggaccagata
     1201 ttcgccatag cccatatcct ccggttcgcc ggtgccgggt gcgctgggca gataatagga
     1261 ccaagccgtg tccaaataac ggactccggc atcgagcgcg gcatggatgg tctcgattgc
     1321 ctgatcacgg ttggaggggc gcccctcgat ggcgagcgcc atcgtgccca tgccaaggcg
     1381 gggaatctca tagccggcaa gtgttgccgt ggtcgcgctc tggttcgtgc ggctcgtagt
     1441 cgtttcgctc atagcagtca cttaatctcg ctcgctggac agtcggaacg attccgtctg
     1501 gggtgaatca tcgtcacaaa cgacggcatg aatatgaata tggctcaccg gaggttgccc
     1561 ggtgagccat agctggctcg actgtcgcct cgcaccccgt ctacaaacgg ctctgccgtt
     1621 tgcttgacga cggggtccat gccaaatcat caagtaactg gttggtccag aggactacct
     1681 ggtcggagct catgggacct tggctcaggg agccggtgaa cggggtgggg acgatgagtt
     1741 ggtgggtgac cgggcggtat tgggtgcctt tgtaaatacg gcccatacgc atctggatgg
     1801 attcgggcgg gtcgatgcga ccgatgcgca cgcggttgga ttgcgcgaga atctccgaaa
     1861 tgccgagctt gcgggccttg aacttcagac gggccacgtc gaacagggtc tcgaattcga
     1921 ccggcggctt gccgtaacgg tcggtaagtt cctcttccaa atccttcaga tcggcctcgt
     1981 tgcgggcgga ggcgagcttg cggtacgctt ccagacgcag ctnnnnnnnn nntactcgat
     2041 cggaatcgac gcttcgatgg gcaggtcgat ggacacggcc accggctcga cgttctcttc
     2101 gggctccttg tacttttcca cggcttcgga caccatgcgt acgtacaggt cgaagcccac
     2161 gccctcgata tggccggact gctcgtcgcc cagaaggtta ccggtgccgc gcaattccaa
     2221 gtccttcatg gccacgtcga aaccggagcc gagcgccgtg ttctgcgcga tggtggcgag
     2281 ccggtcatgg gactgctcgg tcatcggctt ggacgggtcg tacaggaagt aggcgtacgc
     2341 gcgctcgcgg ccgcggccga cgcggccacg cagctgatgg agctggctca agccgaaacg
     2401 gtcggcatgg tcgacgatca gcgtgttcgc attggagata tcaagaccgg tctcgatgat
     2461 tgtggtacac acgagcacgt ctatgtcgcg atgccagaag tcgcggatga tctggtcgag
     2521 ctgcttctcc cccatcttgc cgtgggcgat gccgacgtgc gcctcgggca ccagcgtgtg
     2581 aatcttgtcg gcgatggacg agatgtcctg cacacgattg tggacataga acacctggcc
     2641 gcctcgcagc agttcgcggc gtacggctgc ggtgacctgc gcatcctcgt aggcgcccac
     2701 ataggtcagc accggcaacc ggtcttcggg cggggtggcg agcgtggaca tctcgcgaat
     2761 gccggtcacg gccatttcca gcgtgcgcgg gataggcgtg gcagacaggg acagcacgtc
     2821 cacgttggtg cgcagggctt tcagcgtctc cttgtgttcc acgccgaaac gctgctcctc
     2881 atcgatgatg acgagtccca gatccttgaa cttgattttc gggttcagca gcttgtgcgt
     2941 gccgatgacc acgtccaccg tgccggtttc cagcccctcg atggcctcgt tgatctcttt
     3001 ggtggtctgg aatcggctca tggccgccac attgaccggg aaaccctcga atcgttcgct
     3061 gaacgtctcg taatgctgct ggacaagcag cgtggtgggc acgagcacgg ccacctgctt
     3121 gccgtcctgc acggccttga aggcggcacg cagtgcgatt tcggttttgc cgaagcccac
     3181 gtcgccgcag atgaggcggt ccatcggcac gggcttttcc atgtcggatt taacttcgtc
     3241 gatggtggtg agctggtcgg cggtttcctg ataggggaag gcgtcttcca gctctttctg
     3301 ccatggggtg tccgggctga acgcgaagcc cttggcgcgc tggcgggcgg agtacagctt
     3361 gatcaggtcg tcgg
//
LOCUS       DAWBMW010000173         3351 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_5832_length_3351_cov_2.296723, whole genome shotgun sequence.
ACCESSION   DAWBMW010000173 DAWBMW010000000
VERSION     DAWBMW010000173.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3351)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5832_length_3351_cov_2.296723"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    185..194
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          599 a         1037 c          923 g          782 t
ORIGIN      
        1 acctgaagca catcggttcc gtcgcttcct tcttcgtgtc ccgcgtggac accgctgtcg
       61 acaagctgct ggaagccaac ggctccgacg aggccaaggc tctggagggc aaggccgctg
      121 tggccaacgc tcgcctggct tacgaactct tcgagaagaa gttcgccgag gatccgcgtt
      181 gggcnnnnnn nnnnctgcaa gtacgttgac gagctcgttg ccaagcacat cgtcaacacc
      241 atgccggaga agaccctgaa cgctctggcc gaccacggca acggcgctcc gtccatcgag
      301 ggcacctacg aagagtccca cgccatcatc aacaagctgg ctgagctcgg catcaacctc
      361 aaggacgtca ccgacaagct ggaggccgac ggtgtcgctg ccttcatcaa gtcctgggat
      421 tccgtgctgg ctgacgtcca gtccggcatc gaccgcgtga acgcctgaga tttgacgact
      481 gatgcgtgtt tagcgcacta gttgattagc tcacagaagc tccgacccca accggtcggg
      541 gcttctttcg tttgttgcta cagtggtacg agcatatgag agaaaggccg aaccgtaatg
      601 aacaaacttg tcgcacgcca gcggttggtg atgaccttca ccttcttttc gatgttcttc
      661 ggagcgggca atctgatttt tccgccgttc gtcggcgcgc aggccggttc cgcgaccata
      721 ccggcggcca tcggtttcat cgtctccgca gtcggcctgc cgattctcgg cgtgctggcg
      781 gtcaccttcg ccggcggttt cgacaaactc gccagccggg tctcccccaa attcgccttg
      841 ttcctcggcg tggcgatcat gctcaccatc ggcccgtgct ttgcgattcc gcgtacggcg
      901 accacctcat tcgaaatgat ggtggctcct ttcgtaccgt cctcctatgg ctggctggcc
      961 caactcatct actcgctcgt gttcttcaca ctggcgttcc tgttcgccca gcatcccgag
     1021 aaactctcca aagggctcgg ccgattcatg ggaccgttgc tgctggtgct cattgctgtc
     1081 ctgttcatcg cctgcctgat ccacggcatc ggcatcccgg ccaacccgat gggcgactac
     1141 tccacaaacc agatcgcacg cggcttcctc gacggctacc agaccatgga cctgctcgcc
     1201 gcgctctact tcggcatcgt gatctccgcg aatattcgcg cccaacaagt cgatgatgaa
     1261 tcgcaagtgc agaaggagac cgcgtatgcc gggctcggca ccggcgtact gctgatcgtg
     1321 atttacggcg cgctaagcta cgtgggcgtg gtgtccggcg ccatcgccac catcgacccc
     1381 accaaagaca cgggcgcgac cgtgctcacc aacctcacct catccctgtt cggcacgttc
     1441 ggtaccgcgt tccttggcgt cgtgttcgtc atcgcctgcc tcaacgtatg caccggactg
     1501 atcggcacct gcgccaccta cttccatact cggttccgga ccgtcgccgg ccgaacgttg
     1561 agctaccgca cctggcaggt catcttcacc gtgttcagtt tcatcgtgtc caatgccggg
     1621 ctaagcgcga tcatcaaggt ttccgtgccg gtgttgtccg cactgtatcc gatcgccatc
     1681 gtgctggtac tgctgacgct gacccacaag tcgctcggcg cgcgattccc gcgcgtctac
     1741 ttctggaccg tgctgctggt gggcatcgca tccttcgcca cctgcatctc cagtttggtc
     1801 gccgtcttcg gcggttccat cggctggctg gacgcggcgc tggctatgct gcctttgcag
     1861 caataccagc tcggctgggt ggtcccggct atcctcggtg ttgccattgg tatcgccgat
     1921 gctccgcgac ggtagttctt cccggtctgt gccggagtct ggcggtcggt cggtgatggc
     1981 ggctgccgca gtatccctcc tataaagcgg catacagaat tcctgtatgc cgctggtctc
     2041 cgcccatctg ccatagttat actagcatca tcgacgacgg cgataacggc ataaggtggg
     2101 gtgagtcatg gacaccagtg atagacagat agcggaaacc ggccggaatc gcgagcttgg
     2161 gctgattccg gccatggtga tggcggttgt tgcgacgctc agtggtgccg gtgtatcgtt
     2221 cgcgccatta tatgagcgca tttggggcgg catgatgttg catccgaatt gtccggcgat
     2281 tggcagaatg acctactatc tctcgggcca agcaactgac gattatggag cctgcgtcac
     2341 ctcaccactg tggtttatga cggtgactgc gctgagtgtt gtcgcgattc tctgtctgac
     2401 tgttgcggga gtacagggct tttcgcgcca tcggctctgt tcggtgttta gtgttcttat
     2461 tgcggcagtg gctgttgcga tcatattggt gttgagtgcg aataccaaga ttgtaacgtt
     2521 gtgggcgatg tatgcggtga cgtggctggg gcacgccata gtcacctcat atctgagcaa
     2581 cggttggata cgaatcgcgc gactgttgtt cgtatggcag attggcgtga tgccggtatt
     2641 cgtcaccgaa tattcagtat ccgacgaggt caacttcagc ggcggcgcaa ccatcgcttg
     2701 cggacttgga cttgcggcat atgcgctggt gcaatgcgcc tctcttacca tcgactcgca
     2761 aaacaacagt aatcgaatca cgccagaacg cagtggtata cgggctacct ccttgcacga
     2821 cctgttccac accatcagca tcaacgcatc cgccatacgc aattgctctg ttcccgttgc
     2881 cctgttctgg tcaggtatca cgttctttac ctacagctac aacactggta gacacaccga
     2941 tttctgccca gcgctatcgg caactgacaa tgcaggtctt gtcgcttatt gcatcggcca
     3001 tgaagctttg ggactgctga ccgtcgtgtt gtgcgtgctg ccattgttct ccctgctttc
     3061 ggcgattacg gccatcacgt tggctgcacg caacgcaaat aacggcggta acgctgccaa
     3121 tgccgatggc tctcgaaatt cagccgccgt gtgctatctg ctcctggcaa ccgcactgtc
     3181 cgccacgtcc ttcgtcatcg ccttcacctt ggcatagcgc ggaccaccat gccgcggttc
     3241 gcgcgatcgc tgggatacag cgcttcctgc gcacgacgcg acaccaccgg cataagatct
     3301 ccttcatatc acgaaacaat cgagcgtgtt cgtttgcgcc aaggatcagc t
//
LOCUS       DAWBMW010000174         3339 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_5852_length_3339_cov_2.978685, whole genome shotgun sequence.
ACCESSION   DAWBMW010000174 DAWBMW010000000
VERSION     DAWBMW010000174.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3339)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5852_length_3339_cov_2.978685"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    882..891
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          661 a          828 c         1070 g          770 t
ORIGIN      
        1 tagcgcactt cgttcacacc gaaggggtcg taggttcgat tcctacatcg cccacccgat
       61 atcacgctcg tcagcctatg atggcgggca ttttctatat ctgccgtgcc acggttcctt
      121 atatggcgtc cggtttcggt ggcatctggc tgtgagcggt ttccgattat gagtggcgtt
      181 cggccgtcgt gcaatcccgc tgcgcgcggt gccgtttggg ctgtaatacg ggtgctttga
      241 accggccata acttcttcat atgtcccggg gcttgtgtcg tgtgccgcgg cgcgcttcca
      301 tagtggatat ggcggtgcgg aaggggagcc gccatactta cttacttact tacacaagga
      361 cgtcttggtt cgccagggca acgcgcatgg aaaggggttt gcgatggtgt gcaccacgct
      421 gaatttcgcg gagaaatacc tgctgaaaac cgaagtcagg tccgttgccg gtatgtccgg
      481 cggtacttcc gatgctggta ctgatttcga tgtcgatctc ggtggcgagg gcgacctcgg
      541 aagcgatcat gccaccggtt tcaatcgctc gctatcggcc gacgattatt gggctgcagc
      601 cggatatgcc gatgagtcat tgtattgatg gacgtgatga cgcggtaagg gcgcgtcgat
      661 aagtgcggct ggtgtcgcgg ctgatgccgc atctggtgct cgttcggtgg tcgcggctga
      721 tgttccgtac ggagcctgca atggttttgc atcggtggtg cgtcaaaatc cggaccgtcg
      781 ccgacgctgt ggacgtttgg cggcgttgaa aggacagata atggattgtc aacgccgctg
      841 agaacgattg gcgacggtgg tgggacagcg gatgccccac cnnnnnnnnn ntctcagcac
      901 gtttgcgcgc gtcggatcct tctggagcag caggaaaacg agcgccgcga cgagcaggac
      961 ggcgatggag agcacgccga acgaggcgtt gccggtcagg gacgtggtgg tggctaccag
     1021 gaaggtgccg atgattgagg ccgttttagg gaagaaacaa tagtgagacc ggggaaccct
     1081 tgaaatcatt ggggaatcat ggtggcgatg catgcgatta acacatgttg ttaacacaga
     1141 tgtggaatga ggaggcgcgt gaggctcgtg gtctgcgacg catgagtgtg accgtgagct
     1201 ccccagctga tgagcgcaaa ggcagagatt ggtttcacgc gggacttgtc tgccgaagag
     1261 cggcgattga tgcagggtct gtgcgatgct ctgcagcagt tgctgaacaa gcagtaccag
     1321 cggcacatgt gggactgttc gatagcctga aaaattttgc cgcagacaat ctaggcaatg
     1381 tgcttggcgg ggatttcaat ctcggttcga ttggtgattt gccggcggta atcggtgagc
     1441 atttcaatgc cgatcagttg ggtgaaatca acaggtcgtt ggcgaccatt tgaacgccga
     1501 ccgacttgac gagctgcagg cgctggcgga ggaaaacatc tccgccgacc agatcagtca
     1561 gatcgctggc gcaattcagg agcagggtgc caatctggac ctcggatcca ttgccgaaca
     1621 agcaggactg cccgctgaca tcatcagcca gatttccgga ctattgggca agtagtcgcc
     1681 ttaagcttta acgctgacgg tgttgtctcg gctattgcgt gttttgcggt cgctcagcaa
     1741 atcatatggg tcgtaggtaa ccgattgcga gcgctgctgt acacagatgt gtacgtgggt
     1801 cgaagcggat tcgtggtggc aggtgagaca agctgccctc gcatggctgg aaaagcttcg
     1861 gtctcgtgga gtgcgatgtt aaagctgctc aggtcaacgg aatggcggaa ttccgccgat
     1921 gcaacttgtg gttgcgctcg aataaccggc gattgcaacc caaagatggt ggcgggtgac
     1981 atggacggca tgccatagtg agcccatcgg ccgaacatga catgataaag attacgtatg
     2041 aagggacatt ccaatgagtt ttcgcgctaa cctgcagtac ctacgtgcgc aacgcaacct
     2101 gacccaggaa cgattggcca tgttgcttgg cgtctcgcgt caggccattt cgaaatggga
     2161 atccgaaaag gcctatcccg agatggacaa gctgcttatg atctgcgacc tgttcggctg
     2221 cacgcttgat gatctggtac ttggcgatgt gagtcggcct gccgcgtcgg cttctgcggc
     2281 cggttcgtcg aatgttgact cctcggctga gacggcatcg cctttagccg cttcttccaa
     2341 aacggcgggc ataatcgctc ctatcgcgga gcttgcgcag gatatcactg gctacgatga
     2401 acatcgccgc cgattcgcgc tgctgattgc cggcggtgtt gctgcgattg tcgccggggt
     2461 tggcatcggc aacctgttcg attccggcaa ttccattctc ggagcgacgc cgctcaacga
     2521 ttttctgacg tttctgtgcg tctgtgtggg tgtgattgcg gggctggcga tgctgattcc
     2581 aggaggtctg tcgcgtattg acttcaaacg gcgtcaccca tacgtagaag atttctacac
     2641 cggggaggat cgcagccgag aattgcggct gctggtaatc ggcatcgtcg gtggcatctc
     2701 ggctattctg attggcattg cggtgacggt gtatgccgat gacatgctcg gcgtctccga
     2761 tggctggccc aacgcaattt ttctgctgct ctgtgcgtcc ggtgttttcg ggtttgtcta
     2821 ctgcggcatg agatacagtc tgctcaatat caacgcatac aacaaagcgg ccgaggacga
     2881 ccgcaaggag catgcgggcg agcaggattt ctacgccaaa ctgaccggcg cggtatgcgg
     2941 catcatcatg atgatagcca cgctgattgg cctgtgcttg ctgttcctga gcccagctgc
     3001 tctgcgcggt gattggtcga ccgccggtac aggcatgttc tgggtcgcat ggcctatcgg
     3061 cggcgtgctg tgcggaatcg cggccatcgc catccaactg ttcaaggatt atcgcgagcg
     3121 ctgattgcgc gtcgcaaatt agttagtttc gcgtcgggtc tttctgcagc agcaggaaga
     3181 ccagcgccgc gacgagcagg acggcgatgg agagcacgcc gagtgaggcg ttgccggtca
     3241 gggaggtggt ggccaccggg aaagtgccga tggttgaggc ggtcttaggg aagaaacaat
     3301 agtgagaccg gggaaccctt gaaatcattg gggaatcat
//
LOCUS       DAWBMW010000175         3332 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_5870_length_3332_cov_2.295087, whole genome shotgun sequence.
ACCESSION   DAWBMW010000175 DAWBMW010000000
VERSION     DAWBMW010000175.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5870_length_3332_cov_2.295087"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          695 a         1008 c          966 g          663 t
ORIGIN      
        1 cgggacgcac gtcgagaccg cccatgatgg cgtgcaactg caccatgaga ccgccggtga
       61 gcagtcatga ttcggcaatg cgctcggcca ccatgtatac gatattccac ggatgagcgc
      121 cgctggggat tgcaatgcgg gaaatcattg ctcatttcct tgttgtatgc cgtgatgagt
      181 tctgtaaggg tttcgagccc ctgcgcgtcg ttctctcggg tcgttggatt cggccagatc
      241 agcggcgcat acgccggcgg gaggtctcgt actggcccca gcccttctgc tcggtagtgg
      301 taccgctaca ccgactctcc ccgacactcc agtgccgccg atatcagtga atgcccagcc
      361 tgatgtcggt agagtaaagg ctattggctt ggtctcgacg gcgagacttc tccaatggcc
      421 aatatgcact gttgcaaagg agcatgcggt atgcaaccaa tcaaagccat tcaatacggc
      481 tgcggcaaaa tggcgaagta cacgattcgc tatatgcacg agaaaggcat tcaaatcgtc
      541 ggtgccatcg acgtgaaccc ggaagtcgtg ggcatggatg ccggtgtgtt tgcaggcatc
      601 ggccccatca atgtgccgat tcgcgccgat gccgaggctg tactcaacga gtgcgacgcg
      661 gatatcgcgg tagtcacgct attcagcttc gagaacgaaa tcgccaccat gtgcgagcag
      721 tgcttggccc gcggcatcag cgtgatcacc acctgcgagg aatgcatcta cccgtggacc
      781 accgatccgg ccaccacgaa ccgtctcgac atcatcgcca aggaagccgg tgccaccatg
      841 gtcggctccg gcatgcagga catctactgg gtgaacatgg tctccaccgt cgccgccgga
      901 tgccacagca tcaagaagat caccggtgcg gtgagctaca acgtcgagga ttacggcctg
      961 gctttggcca aagcgcacgg ctgcggtctg accgccgagg agttcgaggc cgagctcgcg
     1021 catcccgaaa ccgtggtgcc ctcgtatgta tggaattcca atgaagcgct ggcccagaag
     1081 cttgggctta ccatcgcatc gcagacccag gccagtgtgc cgtatttcag cgatgtcgac
     1141 gtgtattccg aaacgatggg cgagatgatt ccggtcggca aatgcatcgg catgtccgcc
     1201 gtggtaacca ccgaaactaa tcagggcatc acgttggaaa ctgcctgtat cggcaaggtg
     1261 tacgggccgg atgatggcga tatgtgcgat tggaagatcg agggtgaacc ggacgtggag
     1321 ttccacgtgg tcaagccggc gaccgtcgaa catacgtgcg ccacaatcgt caaccgcatt
     1381 ccggacgtgc tcaacgcgcc agccggatat gtgacggtcg atcagctcga tgagatttcg
     1441 tatcttacgt atcccgccgc gctgtacgtg gcatagcctt catattttca aggatgaagg
     1501 ccggcactca taagggtgcc ggccggtatc aggcggccag agtcgacgtg cgtggcatca
     1561 cctggaactg gatatggtca tgggtgatgt catcgggccg ttcatgccgt aggttcgact
     1621 cgttcagcgt caccatgcga tcgataaccg tacgagccat tgcggagacg tccatcgcca
     1681 cagtgctgat gggtgggttg gtgcaggcgg agaaggggat accgtcgaat cccatgagcc
     1741 gtacgtcgtc gggcacgtgg atgccatgtt cggtgagttc gcggagcgcg ccgatcgcta
     1801 tggtgtcaga catgcacagt tctcatgctc ccgctggcgg gagctgtcat gcgaagcatg
     1861 actgagggtg gtccacagcc gccaatccgt aacatcaccc aacgcagcat ttcacgatac
     1921 ggacgtttcc gtatcgtatt ttcgtgtggt caaatagaca tttcgagcga aaaagttcga
     1981 aataccccca taactggcca taaaaaaaat tgcttgagaa tcactattga cttttatggc
     2041 gactcggtat atggacacat gaaacgtaaa tgacatggag caacggtact gtgtcaacag
     2101 caatgaacga agaggcatga ttttcttacg tgcccacaac caattgaggt gatttcggtg
     2161 acattcaaag ccccgctcga tctcacggtc cacgctccgg acggtatgta tgatcccgcc
     2221 aacgagcatg acgcctgtgg tgtgggtatg gtcaccactt tgaacaagcg tcctgagcgc
     2281 aagatcgtca ccgacgccat tgaggtgctg gtcaaccttg atcaccgtgg tgccgtgggt
     2341 gccgaggaga acacaggtta cggcgccggc attctgatga gcatgccgga tgagttcatg
     2401 cgtgccaccg tgcccgccga actgccggaa gccggtcact atgcggcagg catcgccttc
     2461 cttgaccgcg acatcgaaac ctccggtcag caggaacagg ccattgccaa gatcgtgcgc
     2521 gaggagggac tcgaagtcct cgcatggcgc gtggtgccca ccaacccgga cggccttggc
     2581 ctgcaggccc ttgccgccat gccaggattc aagactctcg tggtcgcctc tccggacgga
     2641 gctctggccg gcgtggactt ggatcgcaag accttccgca tccgcaagcg cgtcgagcat
     2701 gaggtgggcg tctacttcgc atccctgtcc gcccgtacca tcacgtacaa gggcatgctc
     2761 accaccatgc agctcaccca cttcttcgag gatctgaacg acgagcgcat gaaggccacc
     2821 atcgccatcg tccactcccg cttctccacc aacaccttcc cgagctggcc gctggcccag
     2881 ccgttccgtc tgctggccca caatggcgag atcaacacca tccagggcaa ccgcaactgg
     2941 ctctccgccc gtgaaggccg cctgagcagc gaactgctcg gtgaattcga gccgctgctg
     3001 ccgatcacca ccccgggctg ctccgattcc ggcaccttcg acgagtgcct cgaactgctg
     3061 cacctcgccg gccgctccct gccgcacgcc atctgcatga tgctgccgcc ggcttgggag
     3121 aagaacccgg atctcgatcc ggacgtgcgc gccttctacg agtacaacaa cacgcttatc
     3181 gagccgtggg atggcccggc cgacatcgtc ttcaccgacg gcacccaggt cggcgccctg
     3241 ctcgaccgca acggcttccg tcccggccgc tggcagctca ccgatgacgg ctacatcgtt
     3301 ctggcctccg aggccggcgt tctgccggag at
//
LOCUS       DAWBMW010000176         3306 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_5929_length_3306_cov_2.543833, whole genome shotgun sequence.
ACCESSION   DAWBMW010000176 DAWBMW010000000
VERSION     DAWBMW010000176.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3306)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5929_length_3306_cov_2.543833"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          673 a          989 c         1034 g          610 t
ORIGIN      
        1 gcaccgtggt gcgcacgagc tggccgcccg tagcaccatg gcctttgagg aggcccgagc
       61 caaggtggcc aagctggtcg gcgcgaatgc ggccgaaggt gaagaggagg tcgtcgtcac
      121 cggtggtgcc acggccggac tgaacctgct cgccaccgca ttcggcaatg catcgctggg
      181 gcgtggggga gaagccgcca agcgttttgc cctgaagccg ggcgatgaga tcgtcgtctc
      241 gcgtgcggaa catcattcgg tgcggctgcc gttccaggag ctgtcctacc gtaccggcgc
      301 caccttcaag tggatcgacc tgaccgagga cggccgtgtg cgtacggaca acctcgacga
      361 agtcatcacc gagcgtacca aggtcgtggc cgtcacccac gtgggcaacg ccacgggtgc
      421 catcaccaac atcgccccga tcatcaagcg tgcgcatgaa gtaggttcga tcttcatctt
      481 ggacgcttgc cagtccgtac cgcacctcaa ggtcgatttc cacgcgcttg atgtggactt
      541 cgccgcctgg agcgcgcaca agatgtacgg tcctaccggc gtcggtttcc tgtacggtaa
      601 gcgcgacctg ctcgaagccc tgccgccggc caacttcggc ggctccatgg tcgagctcgc
      661 ctacatggac cacgaggccc aatacatggc ccctccggcc cgcttcgagg ccggcaccca
      721 gccggtcgcc caagtggtgg ccgcaggcgt cgccgccgaa tggatgatga acatcgggct
      781 cgagaacatc gaagcccatg agaagaccat cgccgccgaa ctgctcaagc tcggcgacgt
      841 cgatggcatt cgcatcctcg ggccgcgcga aaacgtggac cgcatcggca ccgtggcgtt
      901 cgacgtggcc ggcgtgcatc cgcacgatgt gggccagttc atcgacgccc agggcatcgc
      961 gattcgcgtg ggacaccact gcgcccagcc ggtacaccgc cacttcggcc tatacgcatc
     1021 caaccgtgcc tcctccggtg tctacaattc ggtcgaagat gcccaagcgt tggtcgaagc
     1081 ggccaagggc atccgtaagt tctttggagt ggagtaaaac agaacaccat gaacgaattc
     1141 ggtatgagcg gggatgacct cgaacagatg taccaagagg tcatcctcga ggcgtcgaaa
     1201 catccgcatg gcaaggaatc ctttgcgccc aacgcagcgg tcgaacagtc cgcggacgca
     1261 gcggccaatg tcaccgtaca agccagccac gaatactgca cgcccggtga atcgcaccag
     1321 ttcaacccga cctgcggcga cgaggcgacc atccatgtcg aagtctccga tgacgaaccg
     1381 cacaccatca agcgattggt gtgggacggc cacggctgct ccatttcgca ggccagcctg
     1441 tcggtgatgg tcgatctggt ggacggcaaa accgttgacg aggccatgga tctggaacag
     1501 acattccaca agctgatgga atcgcgcggt gccggtctgg acgacgagaa cctggaagaa
     1561 aagctcggcg acgccgtggt attccaaggc gtgtcgaaat accctatgcg catcaaatgc
     1621 gctctgctag gctgggaagg gctgaaggac tccatcgcca aagccctcgc tgcaaagaac
     1681 tgaggaaata tgtccaacga taatttggtt cccgaaccac agccgtccgt tttcgatgct
     1741 gtcaacggcg tcctgcagga cgaggaggcc gctcgccgcg tgatggccaa cccggccttg
     1801 gccaagggct tcccgaacgg acgcccgatc gagcactctg ctgcgtccgg cgatgacacc
     1861 tgtgcgtgtg gcggtcacca cggcaaatgc ggttgccagg atgacgaaca gtccgatggc
     1921 agcgagattc cgatcaaggc gattgacgat atcggccgcg ccactgccga agacgtgcgc
     1981 gaggcactgc atcaggtgat tgatccggag cttggcatcg acgtgatcga tttgggtctg
     2041 gtctacggca ttgagatcga tgagctaggc cgcgccatta ttacgatgac gctgaccacg
     2101 ccggcctgcc cgctgaccga ccttatcgag gacgaatgcg catccacgct ggccggtctg
     2161 gtcgaggagt tccgcatcga ctggacgtgg gatccgcgct ggaccatgga gcgcatcacg
     2221 cccgaaggcc gcgaccagct cgccgcccta ggcttcagct tcgacaatat gccaaagtac
     2281 tgatagcgct tagtaagttc cctcatcaga gggagctcta tacgagaaaa gccccgcatg
     2341 atgcggggct tttccatgtc tggactggtc aatcagtcat cgacgatggt gttcttcggg
     2401 acaaccgtga tgccatccgg cgtgacggtg aagccacggg ccagatcgtg ctcggtatcg
     2461 atgccgacag tggagttctc ggtgagcacc acgttcttgt caagaatcgc cttgtacacg
     2521 cgggcgcggc ggttgatgac tacaccgtcg aacagcacgg aatcgacaat ctgtgcccac
     2581 gagtggatac gcacattggg ggagagcacg gagtggtgca cttcaccgcc ggagacgatc
     2641 acaccggggg agacgatcga atcggtggcg tggcccagac ggtcgcggcc ggcgtgcacg
     2701 aacttggccg gcggcaggtt gccggacatc gtgtaaatcg gccaatcctg gttgtacagg
     2761 ttgaactcgg gcacataggc gatcagatcc atgtgcgcat cgtagaactg cttaatcgta
     2821 ccgacgtcgc gccagtaagc gtgatcagtg gccgtggagc ccggaatctc gttcgagttg
     2881 aagtcgtaga cgccggcctc gttgcgggaa gcgaagtacg gggcgatgtc gccgcccatg
     2941 tcgtgcttgg tgtcggcagc cttctcatcg agagccagag cctcgaacag ggccttggtg
     3001 ttggcgacgt agttacccat ggaggccaga atctggttcg gattgtccgg cagaccggtg
     3061 gtggtggccg gcttctcctg gaagttcttg atcatgttcg ggtgatcggg atcgacctca
     3121 atcacaccga actggttcga ttcctcgatg ggctggcgga taccggccac ggtgaactcg
     3181 gcgccggact cgatgtgctg ctgtaccatc tgttcgaagt ccatgcggta gacgtgatcg
     3241 gcacccacga tcaccacgat gtccggctga acatcctcga tgatgttgat ggtctggtag
     3301 atggcg
//
LOCUS       DAWBMW010000177         3267 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_5992_length_3267_cov_2.376712, whole genome shotgun sequence.
ACCESSION   DAWBMW010000177 DAWBMW010000000
VERSION     DAWBMW010000177.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5992_length_3267_cov_2.376712"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1384..1393
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          703 a          985 c          924 g          645 t
ORIGIN      
        1 cgcgccagtt cgtagaattc gggcacgcgc ttcatgttgc cgatgaagat gaagaacatg
       61 cagaaggtga gcggcagtcc ccaatccacg tatttgaata cgcgccgatc ggtgaacagg
      121 aacacgacga ccacgatcac gcacatcacc cacaggggaa tcacgtcgga gacggccagt
      181 aggcagacga tgaacagcag cgagtagacg atggtgcgcc agccgccata accggcgccg
      241 tagccggtga tacgaatctc gtcgggctgg tgtttgttgc gctcgggagc cagcacgccg
      301 gtttcgaggt cgcccaggtc gccgaccggt ttgccgccaa acaccacgga aatcacgatg
      361 accagcaaca cggccgccgt gccggaatac ggtgccatga tgccgaggaa ttccacggtc
      421 ggcatgccgg tcagggcttt gagatacagg ttgtgcgcgt tgccgatcgg ggtgagcata
      481 ctgcccacgt tcgcgccaat ggtcatcagc gtgaccacga ggatggtctt atcctcttgc
      541 ccagccatga tgagcacggc caccgcgaac ggcacaaacg tgaccagcgc aacgtcattg
      601 gtgatgaaca tcgcggaaaa gaacgtcaaa gcgacaagcg taatgaccag tccgcgcatg
      661 gtgctgacct tctccagcaa tcgcgaaccg atgatgcgga acacgccgat acgctggaat
      721 ccgcacacca caatcatcag gcagatgagc tggctgatgg tgctggcgtg aatgtagctt
      781 ttgtaatcgg cgtccggcgg cacaatgaaa cacgaaatca gggcgagtat tgcggcgacg
      841 atgagaatcg tctcattttt ggctacattg atcagccagc gtcgcatcca ttcctccgtg
      901 aataacccaa tatcgcaacc attctatttt acgcgcagac cagcataagg tacgtagtcg
      961 cgttgcgctg gcggacatga gtttttggac cggtgccgca catcagccgc gctcgttttt
     1021 ttatttggac aaggaaacat tgagtttgca tgtcttccat acaatgaact gccgcgcaat
     1081 gctgtgcccg tatatacggc tgcatcattg tgtggcaggc cagacacgaa taccgtacgt
     1141 ggctggctca taccaatgga acatagtttc attcgaggaa gaggagcgat gaagttgcgt
     1201 attaccccag tattcccggt caaacgtttc gccgccgtgt ctgcacagaa cgtgaagaac
     1261 gtgcagttcg attacacgaa catgcccacg tacaacacga tcaccaagta gcgcgcactg
     1321 ccgcactgat cggcggcact tgccgcatac atgaatggcc ggtgtgctgc ggattcctta
     1381 ccannnnnnn nnnccggcca ttatgatata tgccgcaata cagggggcaa tcagccctgc
     1441 accttacctc cttgcgcgct acggtatccg aagttcatca tctcgctgtt gtaggcgacc
     1501 acgccacggc cgaccgccgg cagaacgatc atgccgccat cgccatgatg cgcggcgact
     1561 tcgtccaaag tggccgtcgc ggcatcctcg acgttctcgc cggagaaacg cacacgatcc
     1621 gacacctgat gggcggcgac caccttgatg aacgcctcgc cgatgccggt gcaggacacc
     1681 gccaccgtgg actggttggc gaacgtgccg caaccaggca gcggcgaatc acccacacga
     1741 ccgtgcatct ggttggtgat gccgccagtg gacgtgccag cggcgatatt gccctcggca
     1801 tcgcgcgcca cggcaccgat ggtgccatgc ttctcccact catcgccacc ggactgagcc
     1861 tcggccaagg actgacgacg ctgttcggtg atgaagtact cgttgctttc ggtcgcgacg
     1921 ccccaatcgg cgatctcggc atcggtcggg tccgcgaaca gcacatgctt ggtctgctct
     1981 ttgacggcac gcgccgcatc gatcgggttc ttcacggtgc ttacgccggc gaccgcaccg
     2041 acctcgccat cgccggtcat caggcaggcg tccatctggg cgatgccatc gctggtcagg
     2101 gcggcaccac ggccggcgtt gaattcggga gcgtcttcca tgacgtggat ggcggcgacc
     2161 acagcctcat gcgccggtgc tccggattcc aacaactggt atccggcatc gagggcgcgc
     2221 tgcagatcaa gttcgacctg ggcgatgcgt tcaggcgtgc tgtgcttgcc acgactgccc
     2281 gctccggcgt gaatgataag cgtgatgccg gtttcgctgc ccgccagttc agtggtcacc
     2341 ggcgaaacgg aggatgtggt catatcgtgt ccctttctgg agtcagatgg atgtgctgac
     2401 gccaacgtat cgttgcttcc gcatgtatga tagccgttga tggccgcgac cggttccgca
     2461 tttcgcatca tcggctcgcg atggcgtgac tcgttactgc gccacctcgc cactacgcca
     2521 ggggcaatgc gatgccggct cgatcggcca ggtcacgcaa cgaccacgtg gtctgccccg
     2581 ttcgcccgac aacgctatgc gcatgccaca gcgaactgat gatggcttcc tcgcttattc
     2641 attcgtcgaa tgcacccatc gatgtgggta tgcagccatc aaaaaaactc gacaaccgca
     2701 atgagccagt cacttgacta catctgtgct atcgtctcgc caatgattcg aatgccggta
     2761 tcaatgtcag cagcatccgg gcgcacaaag ttgagacgga tgtgggaatc atccatttcg
     2821 tcaacgtcga acacgctgcc cggcatgaac gtgacgcctt gggaacggca ggattccaat
     2881 acatccagag agcgcacgcc gtccggtaag gaaacccaaa tatagtagcc gcctgcaggc
     2941 acggtccaat acacttcatc cggcgcatac cgctccaatg ctgcgattgc tgcatctcgt
     3001 cgaatgcggt aagccttaat caaaccgcgc aaatgttcgg cgacgcggcc ttctttaagc
     3061 agttccaggc agatgcgctg cgatgacgtg ctcgtatgct ggtcgaccat gcgccgcaga
     3121 gccgccagcc ggttaaccac atgcgggtcg gccacaaccc atcccgtgcg caggcccgaa
     3181 gtgacggttt tcgagaacgt cgacaaatac atcacgtatc ccgcattctc catgccttta
     3241 agcggtatgg ggcgatgcgc gtcggac
//
LOCUS       DAWBMW010000178         3237 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_6076_length_3237_cov_2.004714, whole genome shotgun sequence.
ACCESSION   DAWBMW010000178 DAWBMW010000000
VERSION     DAWBMW010000178.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3237)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3237
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6076_length_3237_cov_2.004714"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2012..2021
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          597 a          995 c          917 g          718 t
ORIGIN      
        1 cccacacgta gatcgccgtg gccatgccgt ggaacacgat cgacaccgat ccgccggcct
       61 gcttggcgtt tgccacgggg gcggagacgg tgaacttcct gcggtagagc gcgacgtgat
      121 tgttctccgg gatgttcggt tccaacgggt tctcgtggcc gtcccacgga tactggacgt
      181 tcacgtactt gtggttcatc agaccggccg tctgcaggtg gcccggaacc tggatacgct
      241 cgaacgagga gtcgtcgaac gactcggccg tcgcgggttc ctcttcgagg ttgatgtcgg
      301 cggcctggac gacctcgacc cgccactcgc cgtcaattgt ccgctctggg ggtcgcgggc
      361 gtaccacttg tggctggaat gggccggagt ccggttcacc tcgaacaccg tcgggtcggt
      421 cagccaagcc tgcgatgcgc gatcgacatg tgtgacgtct gtcatgctct cctgctttct
      481 gtcattacct cgttgtgaat gatattatgg aagaggccgg ggcctccttt accgaaaagg
      541 ataaacagtt tttatataaa acgcaaatcc tgtttaccgc ttccaaatca ctggcactgc
      601 ttgccgtcga cgctacgccg cgtccatgca atgtctcggt tcagctcatg ttatcccatc
      661 cttgcgacac gctgtttatc tccttgttta ctaatgcttt ttactttcag tgataaaact
      721 tgtatgctta cggtgataaa acttgtatgc tcacggtgat aaaactagta tgcttacggt
      781 gataaaactt gtatgcttac tttacaaaat caatgagtga ttccatgctc tgggttctta
      841 aggagaagat taatgagcaa cgaaaatgtc gcggacggtg acgtccgcag gaagggcggg
      901 ttaggccagc gcatcgccta tgcgtgcggc aacttggggc aggctgcctt ctacaacgcg
      961 atgagcacgt acttcgtcac gtacgtcacc agctgcctgt tcgtcagcta cagcaaggct
     1021 ctcgccgccc aaatgatcgc cgtcatcacc ggcctgatcg tcgttatccg catcgccgag
     1081 atcttcatcg atccgctgct gggcaacctc gtggataaca ccaccaccaa gtggggccgt
     1141 ttccgcccgt ggcagttcat cggcggactg gtctcctccg tgttgatcgt gctcatcttc
     1201 tccggcatgt tcggactggt caacgtgaac accacgctgt tcatcgtgct gttcatcatc
     1261 acgttcatcg tgcttgacgt gttctactcc ctgcgtgaca tctcctactg gggcatgatc
     1321 ccggcgttgt cctccgactc ccacgaacgt tccacctaca ccgcccttgg caccttcacc
     1381 ggctctatcg gctacaacgg catcaccgtc atcgtcatcc cgatcgtcag ctacttcacc
     1441 tggacgttca ccggcgccaa gggccagggc caggccggat ggacctcctt cggcttcatc
     1501 gtcgcgctcc tcggcctgat taccgcctgg accgtcgcct ttggcaccaa ggaaagcacc
     1561 agcgcgctgc gtgccaaggc ccagaagaac ggcaacccgt tcgaggcgtt caaggcgctg
     1621 ttccagaacg accagctgct gtgggtcgcc ctgtcctacc tgctgtacgc catcgcaaac
     1681 gtcatcacga ccggtgtgat gtactacctc ttcgtgttcg tgcttgacga gcctgccgcc
     1741 ttctccatca ccggcatcat cccgctgatc gccggattca tcatggcgcc gctgtacccg
     1801 attctcaacc gctggattcc ccgtcgctac ctgttcaccg gcggcatggt ctccatgatc
     1861 atcggctaca cgatgctcgc cctgttctcc agcaacctgc cggtcgtcat cgtcgccctg
     1921 atcttcttct atgtgcctgc gcagttcatc cagatgaccg cgattctgtc gctgaccgac
     1981 tccatcgaat acggccagct gaagaacggc annnnnnnnn ngtccaacat cgccacgttc
     2041 aagaccttcg ccttctacgt cccgcttgtt ctcatcatcc tcagcctcgt cgtgttctgg
     2101 ttcaaggtca agatcgatga gaagatgcat gcgcagatcg ttgaggagct cgaagccaag
     2161 ctcgcttccg gcgagatcgt cgacgatgag gcgcaggcag ttattaagaa ctgatctccc
     2221 gttcccatcc ctcccataag gcccgtccgc ctgaaccgca agatgcggtc cgtggcggac
     2281 gggcctgttc gttcctgcgg tcggcggtca tgaacatacc catgtcggtt ccggcagtgg
     2341 ggccggaacc ggcggcgtgt cgactatcgg ccaggagtgt ctatcagctg gtatccgata
     2401 tgggccgtgg ccctgcgccg cctgccaaag gagataaccg tgcgtgcatg acgtgacttc
     2461 gtatggccaa aactgctgac cgcggacgga cgcggcatcg ttttcggtgg cgactatagt
     2521 cccgaccaat ggccggagga aatctgggat gacgacatcc gcctgatggg ccaggccggc
     2581 gtcaacaccg tggcccttgc catcttcagc tgagatcgcc tccagcccat cgaagaccat
     2641 tgggatttcg actggctcga tcgcgtcatc gacaagctcg gtgacgtgac cgtcgccgga
     2701 ccgcatgtgc aggtgcttgc ctcgtatgcc ggcgaagaag cggatgagtg ggagcttgac
     2761 ggcatggccg cgatcacccg caacacgtac ggcgaagggg aggcgtattt cctcggctgc
     2821 gatctgggcg tgagcgatct gacgcgattc gtgggaggct ggcttgccgc gcagccgcag
     2881 gatggccggc agccggaagc caacctgcgg tcgcccgcca gcggcgtgac ctctgacgtt
     2941 ctgcacaccg tgcgtcaatc cgacgacgcc atattcgact tctatctgac acgtggcaga
     3001 tcggatgtcg aactgcgcga catcgcaggg gagccgatcg tcctgttccg ggcggaacgg
     3061 ggcagtgacg gcggcgcgta tacggtgcat cgcaatggcg ttttcgtgac gaagcgcccg
     3121 aacccgtcgg tgtagtcagt tgcgacgaaa caggagggcg gagccgcgga cggttttgtg
     3181 agacggtccg cggcctcacc gttggaagcc gcggactcgg atcatcagca acggacg
//
LOCUS       DAWBMW010000179         3223 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_6105_length_3223_cov_4.268939, whole genome shotgun sequence.
ACCESSION   DAWBMW010000179 DAWBMW010000000
VERSION     DAWBMW010000179.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6105_length_3223_cov_4.268939"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          574 a          930 c         1040 g          679 t
ORIGIN      
        1 atgcggccgc gctcgacttc cttgatctgc ttgacgacct cgacgttgat gccgttcggg
       61 tcgtagatgt agccgttgga gtcggagacg gtgacgacct tggcgcccaa ctgctcagcc
      121 ttctgcgcgg cgtaggtggc cacgttgccg gagccggaga tcacgacggt cttgccctcg
      181 aaggagtcgt tgcgcagcac gcgcagggct tcctgggtgt agtagcacac gccgtaaccg
      241 gtggcctcgg tgcgggccag ggagccgccg aattccagac ccttaccggt caggacgccg
      301 gagtactcgt cgcggatgcg cttgtactgg ccgaacaggt agccgatctc acgaccaccg
      361 acgttgatgt cgccggcggg aacgtcggtg aactggccga tgtggcggct cagctcagtc
      421 atgaaggcct ggcagaagcg catgacctca gcgtcggact tgcccttcgg gttgaagtcg
      481 gaaccgccct tgccgccgcc catcggcagc gtggtcaggg agttcttgag aatctgctcg
      541 aagccaagga acttgacgac ggactcggtc acggtcgggt ggaagcgtag tccgcccttg
      601 tacgggccaa tggcggagtt gaactggata cggtagccgc ggttcacctg aaccttgccc
      661 tcgtcgtcaa cccaggccac gcggaacttg atggtgcgct ccggctcgac gatgcgctcc
      721 agtacaccgg ccttctcgta ctccgggtgc ttctcgacaa ccggctgcag ggtctcgaag
      781 acctcgcgaa cggcttgcag gaactccggc tgatcgccgt cacgggcaac gacctggtcg
      841 tagacacgct tgatgtactc gtcagtgagc atgattctcc tcgatttgct agggaattaa
      901 gacggtttta ttctatgcgc gcaggccctt gctgcataga gtctcgctcg gataacggga
      961 aagctatcgg cgtggttatg gggtggttca agtttccgtt tgttagattt ttcggcgatt
     1021 gctggatgtc tgtctgtgcg tgaatcgggt cgaaatggaa gctgcatcac accgaaggtg
     1081 cgttcatata gtggacataa aggtggactt gggtgatgtc ggatgtgccc gttgggaatc
     1141 gtgcgccgta ggggccgagg tcttcggcgc gcaggttacc gggttcgcgg tcagtgcgcg
     1201 aagtaggtct gcagaatcat cgtggtctcg gtgcttaccg gcaccgtgcg gtggatgaga
     1261 ttgagcaatt cctcaagggc ggtgggggag gccacgcgta ccaccagtac gaagctgggg
     1321 gcgccggcca ccgaatagca ggagacgatg ccgtcgatat tgttcagctt gtccggaatc
     1381 gaggattcgg ctgagtagtc gagcggggtc acgttgacga acgcgctgat gggcaagccg
     1441 cgctgctctt ggtcgatgat ggccttgtat cccttgatga tgccgcgctt ctccagcttc
     1501 tgcacgcgcg actgggcggc ggagacggac agtccggtcg cctgggccag ttgcgcgagc
     1561 gtggcgcggc cgtcggcctc cagcatggtg aggatgcgct ggtcggtctc gtcaatcgcg
     1621 gtcatggtgt cttctccgtt cgttgtgctc tgtgttcact gtacctgttg ccggaatgcg
     1681 atggctttcc gattttgcct ggtaatttca gtagttcttc ctgtgttttc tgttgtgtgg
     1741 tggaatgttt atgcctaaag tcctaattga atgtaataat tccttctgaa ataagagaac
     1801 gacgaaataa ttccattgag gagggatgat atgcagaagt tttccaacat gcctctgtcc
     1861 gaagttgcat ctgctgctgt gcctgctgcc agacgggttg ctgtgccgac cgccaagccc
     1921 gcgagcttgt ccgtcgaagc cctgaatatc ccgcgctccg gcatccgaga cgtattcgac
     1981 cgtgtagagc gcgtgcctga cgcgatttca ctgaccgtgg gggagcccag cgccacggcc
     2041 gcgccgcata tcgtggcggc cgcttgcgag gccgcacagg ctggccgcac tcggtacacc
     2101 aacgtgctcg gcgtccccga ataccgcaag gccgtggccg attactccgc ccgtgtgaag
     2161 ggactcactt atgatcccga aaccgagatt caagccgttg acggtgccac gattggcctg
     2221 tttctggcgc tgaaagccgt ggtaggcacc ggcgatgaag tgattattcc ctcgccgttc
     2281 ttcacttcct atgacgccga agtgatgctg tgcggcggcc gcccggtcac cgtggcgctg
     2341 cgtccggagc acggcatgcg cgtgaacgcg gcggacatcg aagcggcgat caccccgcgc
     2401 acccgcgccg taatcatcaa ctcacccggc aatccgaccg gcgcggtaac ttctgccgct
     2461 gaactggcgc gtattgccga agtatgcaag cagcacaata tttgggccat ctccgacgag
     2521 gtttaccatc cgttcgtgtt tggggaaacg ttcggggaaa cgcttggggg agaggcagcc
     2581 gtggcaccat cgattgccgc ggtgccgggc atgaaagacc gcaccattgt ggtggaaagc
     2641 ctgtcgaaaa cctacgcgat gaccggctgg cgcatcggct acctgctggc acccgaagcg
     2701 gtcatcgaac agaccggtaa aatcgccgaa ctcatgcact cctcggtgaa ctccttggcg
     2761 caatacgcag gcgtggcagc actggccggt ccgcaggatc atgtggccgc catgcgcgag
     2821 gaataccgcg ccaagcgcca gatcgtgctg gacggtctgg ccggatgccc ggtattgcgc
     2881 ttgattgaac cgcaaggtgc gttctacgcc tttgtggacg tacgcctgac tggccttgac
     2941 tccggtgaat tcgcagaccg tctgctggac gaagagcacg tggccgtggt ccccggcgag
     3001 gccttcggcg aggaggggcg cggcttcgtg cgcctctcct acgccggaga tgccggcgag
     3061 ctgcgtgagg gcgtcgcccg tctgcgcgca ttcgccgaac gggtatggaa ccccatcacc
     3121 ggccaccaca ccgccaccta tcaccccatg gaggtgctgg cctgaggctt aaagcgaggc
     3181 taggccgcca attcagactc gtcggcgcga gcgatggcct gct
//
LOCUS       DAWBMW010000180         3205 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_6156_length_3205_cov_2.230794, whole genome shotgun sequence.
ACCESSION   DAWBMW010000180 DAWBMW010000000
VERSION     DAWBMW010000180.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3205)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3205
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6156_length_3205_cov_2.230794"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    378..387
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          623 a          968 c          943 g          661 t
ORIGIN      
        1 gtaataaggt catagccctg ggccgcaagg atgcgttcca agtcaagtgt cggggtaatc
       61 atgcgcggct cccatcgtct cacaatggtt aggattcaga cggttcagga ttcatatggt
      121 tcaggattca gacggcccgg aactcaatca gttcaaagac tcacacggtt caaaaaaaga
      181 ctcaggaatt cgagccggaa gcgtcaggca cgtccttgaa gacttcgtgg tggtcatcaa
      241 tcgcaccaag ccggtcaagc ggccagtatt tcgcgattcc gactccgacc acatcggaga
      301 tgggaaccaa gccgcgatcg ccgtcatcct gatgatagcg ggagtcggcg gaattggcgc
      361 gattgtcgcc catgacgnnn nnnnnnntca ttgatggcca cgccgttgat ggtcaccggc
      421 tggcccgcgc ccttgcattc gaccacgtca ccgggcaggc cgatcagtcg cttgatgaga
      481 tacccgccac cgggagcact gctctgctcc tcgttcaacc agttgttggg atctttgaat
      541 accacgacgt cgccgcgctg cagatcgaag atcttggggg tgagcttgct ggtcaccacg
      601 cgatcgccgg gcaccatggt gtccatcatc gagcgcgaag gaatctcgta aaagccgacg
      661 gcgaaaatgc gaatcagcag cacgataagc acgggaacgc cgcaccacag gaaaaagtcg
      721 cgtagggtga agtggtcatc atctgacgat gatgctgccg cggcattcgc gccagcggct
      781 gcacccgcgc cagcggaaga agcggcgtgc gccgggcgcg ccggtgccgg gggaaccggg
      841 gacagggcga tgccatggtc agcgacttgg aacgtgcggg aatcgcgggc atcggcctcg
      901 tcatcatacg aggtctggtt gctctccgga ttgtcggaaa ccgggaattc gccgttatcc
      961 tgatcgtcct gcataccatc tcctcgcgat tcatgctgcc ggatatgagg ggtgccggcg
     1021 cccgtccatc atacgtggaa aatacgaaaa cagcccgaaa ccgaaacggt tccgggctga
     1081 atttcacggt ttattcgcgt gcctgcatca tcaggctcac gattgcaccc gcgaatcgac
     1141 tcacttagcg gagttgtcgc ggcgctcggc aatacgagcg gccttaccgc gcagagcgcg
     1201 caggtagtac agcttggcgc ggcgcacacg gcccttacgc acgagcttga tggagtcgat
     1261 ggacggggag tgcaccggga agcgacgctc cacaccggtg ccgaagctga tcttgcggac
     1321 cacgaaggtc tcacgcacgc cagcgccctt acgagcgatc accacgccgg tgaaggtctg
     1381 gatacgggag ttgttgcctt cctggatctt cacgttcacg tcgacggtgt cgccgggacg
     1441 gaaggccggg atttcctcgg ccggcttcat gtgcttggcg tcaaaagcct caacagcgtt
     1501 aaccattgtt ttccttcagt gctgccgcat atcagccaca gatctggggc catccacgcg
     1561 attgcgcatc gcgcgcgttg gcctgttctc gttatccctc gggtttgccc ttgggaagcc
     1621 tgccgatccg ccaaaagcca ggccttgcgg ccgttcggac tggacccacg gagacgcggg
     1681 cttatgcggc ggcccacttc tcgacacagc aatcgtcaat tgtacggatt attgcggaca
     1741 gaatggctgt cggatggcat tccaacacca aggcactgcg acgtagttga cagacctgca
     1801 ggcgggcttt atcttgggaa cgtcggtagg cgaggctacc gcagggatat gggttgctgc
     1861 cgctaacagt ggagacactg cacgcaggtc agcagttctt gtcggatcaa ggcatgaact
     1921 aacgcaattc caccgccctg cgatgctgaa gcttgaacga tgacacgact aggggtaact
     1981 cttcgagaag tcgcacttcc tcatgtcgtc aatgtccgac ggcgtactgg ttccattgag
     2041 gaggtgaatg tcatggacgg cgatttaggt catagtagcc ggctgcgcga tggcggacaa
     2101 tatcacatgc catcgggcgc agcatacggc ccattctctt cacacgtttg taccgcgacc
     2161 atggcattgg ccgtccgtca cgataccgcc gtatccggcg agggcgatcc gatcactgac
     2221 tgattcggct cgcatcgact tcaccgcctc acgacggctg cgacatcccg catgacgtac
     2281 gcaccgaacg cctgaatcgc gaatccacat cccgttctcc ggactcccgg catatcggca
     2341 tcatcatatg acgacgccga tcggctatct gcatgcagac atacccgcat atcggcgcgc
     2401 ctgcacgcga ccgctgacgt ccatggcgcg cacttgccgc acggataccg agtcgaacgc
     2461 ccaccgaacg ggaaccgttt atctccttcg agataacgac atgagagcgc acaggttccg
     2521 ttatgtccaa cttcttcaat tactcctcgt tccccggcat tcgtctgcgt tccgacgtcg
     2581 acgccgccga catgtcccgc agcagcgccg cgcgccgcat cgccgcagac gtggtggtgg
     2641 ccgcgggcgt gctcgccctg ttcggcgtcg tcgcctggct gctgccgcaa atcaacgaac
     2701 cgatcggccc gtcaggcgtg ccgagcacgg tgagcgacga tttgcatctg ctgccgtatt
     2761 acgcgctgcg ttcgatgttc cgcatgttca tcgcgctgtt tttgtcactg gtgttcagct
     2821 tcatctatgg tttggcggcg gcccgatgcc ggcgactgcg catggtgctg ataccgctgc
     2881 tcgacatcct gcagtcggtg ccgatcctcg gcttcctgtc cgccacggtc accatttgga
     2941 tggtgttgtt ccccggctcc attctcggcg tggaggtggc gtcgatcttc gcaatcttca
     3001 ccagccaggc atggaacatg accttctcct tctaccattc gctgctcagc gaaccgcagg
     3061 agttggatga ggcggctcgt tccatgcagc tcacgcgctg gcagcgtttc tggaagctcg
     3121 acgtgccgaa ttcgatgatt ccgctgctat ggaactgcat gatgagcgtc ggcggcggct
     3181 ggttcttcct gacggcctcg gagat
//
LOCUS       DAWBMW010000181         3180 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_6201_length_3180_cov_2.982720, whole genome shotgun sequence.
ACCESSION   DAWBMW010000181 DAWBMW010000000
VERSION     DAWBMW010000181.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3180)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6201_length_3180_cov_2.982720"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    660..669
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          692 a          879 c          911 g          688 t
ORIGIN      
        1 ccctccttgc ggtaggtcag gtgttgcaac gatcactaga acccgccggt cttctgcacg
       61 ttgcgggggg tggttatggt tcatccagat gagaacagga gcaaacctat gagcaagatg
      121 agtgacatga cggaatacca tgcttcagcc taccgtttgc cgtcaggctt cgaacactgc
      181 tcgaaattga agccggtggc tgaggcagcg acggcgctgg accgggtcaa ggccgtggtg
      241 gatgtgctgt attcgccggg cggctgcccg tgggacggca agcagacgaa caagtcgctg
      301 ctcaagaatc tgctcgagga aacctacgaa tacgtcgacg cggtggagac tcacgaccgt
      361 gacaacatgc gcgaggaact gggtgatgtg ttgctgcaat ccgtgtttca ggcgcgggtc
      421 tgcgaatcgg atactgagga cccgttcggt attgatgagg tcgccgatcg gttggtgaac
      481 aagctcatca ctcggcatcc gcatgtgttt gcggctgatg atgccggcaa ttcctctgat
      541 tcttctgatg cttttgatgc tgacagtaac aacgggggag aagctgccca acccgaatcc
      601 ccggaagctg tgctggctct atgggaaaag atgaaacagc aggaaaagca tcgtaaatcn
      661 nnnnnnnnnt tcccgcgttc aaggtgcgtt gcctcgcgcg gccaaagtcg tttctcgtat
      721 ctccaaatcg ccgaatgctg atagattgtt cgcggcgttt gatgagtcgg ctgcggctga
      781 tgctcagcga gatgatgtac atcctcaagc ggcgaaccaa tccgaatcaa gcggcgacaa
      841 ttccgctgaa cgggagaagg ccgatgccta cgccgatgaa atcctcgccg tcgtgcgcaa
      901 ggcccgcgtt gacggcatcg atatcgagtc cgcactgcgc aatcgcttgc gtgatgtcga
      961 agaaaaagtc gccttgattg aatcagaaat caacaacgat taagtccata catgtttttt
     1021 gatcacggat cgtaaaactt agaggtgctg agttgccaac gactaccggc aagctggcgt
     1081 cattgccatc ctattcacgt cttagtggcc ccttcctccc cggccatact actcaggcaa
     1141 ggcctgttgt aggctcgggt acgacgggct gtagcgccga ataaggatga acgggaacag
     1201 aacgaagacg gactgagacg aaccgaagcg gtaccagagg ggagtggctc atgcaggtgg
     1261 cgttggagaa caacatcgtt tttgtggtca tcgagtattt agcaatcctg tgttggggat
     1321 tatcgggcgg acttgccgcc attcgcaagg gctacgacat cttcaccatc atgctgtgcg
     1381 gatggcttac cgcactcggc ggcggtcttg tgcgcgacgt gatgctcggt gccctgccgc
     1441 cggtcggcat caccgacaaa ggctatgtgc tcaccacgct gttttccggc attatcgtgg
     1501 tggtggccca tccggagatc accaaactca aatggacgat gactgtcatc gatgctctgg
     1561 gcctcggact attcgcagtc agcggcaccg caaaagctct ggcctacggt tcctcaggca
     1621 tgacagccgt attccttggc atgttcacag cactggccgg cggcctcatt cgtgacatct
     1681 tcattggcga cgtgccaatg attattcgag acaaacacct ctatgctgtg ccttcgttca
     1741 tcggctgcat cctgaccgtg ctggtatggc gcggcgtaag ctacggctgg ttcgacatgc
     1801 gctcggaaat gctgctcgac gtgctgatcg tcatcatcgt ggtggcgctg cgactgctct
     1861 cggtaggctt caacgtcact ctgcccggag ccgtcgaacg tcatcgcgtc tacctgccca
     1921 gcgaaagccg atatctcaag cgcccggtca tccatccgca cgccgatacc gtcgatcccg
     1981 gcgataccac caaggacgaa gaagacaagc ctcaggatgg ttcaaggccg cctcagggct
     2041 aactgtcttc aaagaggtac atctggcctt ctgcagaatg ctaactgctt tcaaagaggt
     2101 acctgaagca cgaaacgctg agtataagtg ttgcaatgat ggcgtttata gttggttttt
     2161 cccgtattcg gtacctctct gaacggagat gtcatttcgc aagaccgcca atgtacctct
     2221 ttgaacggag atacaaaaag tcccgcaagc cagatgactt gcgggacttt ctaaaacctt
     2281 aagaggctta tgctcacaga gcgttgatcg caacagcgag gccggacttg cggttggcgg
     2341 cctggttctt gtggatcacg ccagcgccag cagccttgtc gagcttctgg gcggcaacct
     2401 tgtaggcggc ctcggcggca gccttgtcac cggcggcgat ggcttcgcgg gtggcgcgga
     2461 tggcggtctt caggccggac ttcacagcca cgttgcgaag gtgcgccttc tcattggtga
     2521 gcacgcgctt cttctgcgac ttaatgtttg ccacgtttac tccaaacgat gtgtgttata
     2581 aggacataca aaagttagag gatagcatga gccccggaaa aacccgcgca atgccacgcc
     2641 gaatacagtc cctcggctac actaggtgaa gtttgagtat atgccaagag gaggaatgcg
     2701 ttggtcgtcc aacacaatca gccgggttcc acggatcagt cggtgattcg caacttctgt
     2761 atcatcgcac acatcgacca cggcaagtcg accgtggccg accgcatctt gcagttgagc
     2821 ggcattgtgc ccgagcgaga gatgcgcgat cgcttcctcg accgtatgga tatcgagcag
     2881 gagcgtggca tcaccatcaa atcccaggcc gtgcgcgtgc cgtggacctt tgacggcacc
     2941 gaatacacgc tcggtatgat cgacaccccc ggccacgtgg acttcaccta tgaggtgtcc
     3001 cgcgcactgg ccgcctgcga aggcgcggtg ctgctcgtcg acgccaccca gggcatcgag
     3061 gcgcagaccc tgtccaatct gtatatggcc atcgaccatg atctggccat catccccgtg
     3121 ctcaacaaaa tcgacctgcc gtccgcagaa ccggacaagc atgccgagga gatcgcaggt
//
LOCUS       DAWBMW010000182         3179 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_6204_length_3179_cov_2.871639, whole genome shotgun sequence.
ACCESSION   DAWBMW010000182 DAWBMW010000000
VERSION     DAWBMW010000182.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6204_length_3179_cov_2.871639"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          669 a         1021 c          897 g          592 t
ORIGIN      
        1 cggcaacgcg ttcgatatcc ccggattccc ggtcgatacg catgtgatgc gcgtaaccgg
       61 acgattgcgt tggcggtcgg attggcggtc cgcacacccc gatcccgtga aaatcgagaa
      121 ggaaatcacc gcctgttttc cgcccgaaga gtggacgaat ctcagccacc gactgattct
      181 gttcggccgg gccacctgcc acgcacgtac ccctgactgc gccaactgcc cgctgtccga
      241 cacctgccct agctatgcgc cgcccgccgg caagagcacg tccaagcgct aactcatacc
      301 tcatcatggt gaattgcgga ttctaggctg aatccaccgc tagactgaaa accatgcagc
      361 acagcaagcg ggacaataca tggtccaagt gggggatttt tctcggagtc gccgtggcac
      421 tcggcgcact cgtggttgtg ggatggacgt tcatgcaaaa ccggttccag ccgggatcat
      481 ccctcgatgc cgatgtgacc attggcatcg acgatatgcc gcaatccttg gatattcggt
      541 ccgattcctc tgcggcagcc gaacgcctgc tcgtcgacaa tgtgtatgag acattagtga
      601 ccgtcgatca agacaacaag cttcagccgg gactggcgac aagctggaag acttccgatg
      661 acggactgac cgtcaccctg acactgcaat cgggcgtcac cttctcgaac ggccatacgt
      721 tggacgcctc ggacgccgtg tggtcgttgc aacagaacgt gaccaacaag gtggcggacg
      781 tcgacgaatt gggcgatctg gcgtcggtcg ccaatccgaa cgccaccacg gtggtcatta
      841 cgcttgccaa gcccaatccg acgctgctgc gagcgctgtc cggcagactc ggcattgtct
      901 acgattccga gtcgtccagt gccgattatc aacgcaaggc cataggttcc ggaccattca
      961 ccgtggccga tttccagcct ggccattccc tgaagctggc gcgcagcgat acctaccatg
     1021 gcaccaaagc ggccagcaat gtcgttgaat ttatgcaata ctccgacgcc gatgcgctca
     1081 gcaaggccct gaccgacggc agtctcgata tggcggcacc ggtttcggct tcgacggcca
     1141 ccggtttgaa cggcaaggat ggcctgaccg tcaaggaggg ggccactacg gataaggtgc
     1201 tgctggccta caacaacggc accgattcgc tgctctccga cgaacaggcg cgtaaagcct
     1261 tccgctatca aatcgacgca gccggcatcg cctccgccca acccgacgca gccggagcat
     1321 tgggcggccc catcagcctg ctggaacccg gatacgaaga tttgaccgga ctgtacccgc
     1381 atgacgaaaa ccaagctggg cagatgttct cctactttgg tgcgcaatac ctgactacgg
     1441 tgaatctggt ggttcccgaa gaataccggt cgttggccga aaccatcaag cagcagatcg
     1501 agcggcagcc gcgccccacg gtgaaccttg aagtgctctc cgatgaggat tatgccaaac
     1561 gaatcaagga cggcaaatgg gaactgaccg tgatgagcat ggacggcacg gatgatgccg
     1621 gcatattcgc cgaccccgat tccatgttcc attacgacca taccgaagcg caacaggcct
     1681 atgcggatgc tcgcgccgcc actaatgacg ccgattatga ggcacgaatg aaggcgtacg
     1741 cccggctgat cagcgaggat gcggccagtg attggctgta tacccgcaaa tgcttcactg
     1801 tggcgtccac caaggtctcc ggctacccga cctcgatgat caatcgccgc atgccgctgg
     1861 ccgggttgac ggtgaagtag gcaaccttca gtcacggacg ggctacgccc gatagtcctt
     1921 ggctcactat cgttcgatcg ttcgtacctc gcctgcgaac agcctactgg gctgtttact
     1981 gtacagttcg gcttgtcatg caacgtgcat aaactttggc cttatgactg aaggcaaatc
     2041 catcatcaac gccaacctca ccccgctgcc cgacaaggtc ggcgtggacg gacttgagga
     2101 caagtggcgc accgtctggg atgaagacgg cacctacaaa ttccgcaaca cccgcgaccg
     2161 caaggccgtc tactccatcg acaccccgcc gcccaccgtc tccggttcgc tgcacgtggg
     2221 tcacgtgttc tcttacacgc acaccgatgt gattgcgcgc tacaagcgca tgcgcggcta
     2281 cgacgtgttc tacccgatgg gttgggatga caacggcctg cctaccgagc gccgcgtgca
     2341 gaactactat ggcgtgcgcg tggacgtgtc tctgccttac gacccggact tcaagccgcc
     2401 gtttgaaggc actgatggca agaagattga cgccaaggat caggtgccga tttcccgcaa
     2461 gaacttcatc gagctgtgcg agcgtctgac tgctcaggat gagaagctgt ttgaggccct
     2521 gtggcgcaag ctgggtctgt ccatcgactg gtcgcagacc taccacacca tcggtcagca
     2581 cccgcagcgt gtggcccaga aggccttcct gcgcaacctc gcccgtggcg aggcctacca
     2641 gcaggacgcc cccggcctgt gggatgtgac cttccagacc gccgtggccc aggccgagct
     2701 cgaatcgcgc gaataccccg gcttctacca caaggtggcc ttccgctttg aggacggcac
     2761 cccgatctac atcgagacca cccgccccga actgctggcg gcctgcacct cgctgatcgc
     2821 caacccgaac gacgaacgct acaagcagta cttcggccag tacgtctact cccctctgtt
     2881 caaggtcaag gtgccgattc tggcgcaccc ggccgccgaa atggacaagg gtgccggtat
     2941 cgccatgtgc tgtacgttcg gtgatgtaac cgacgtcgag tggtggcgcg acctgaagct
     3001 gcccacccgc ccgatcatcc agcgcaacgg ccgtatcgtg atggataccc cggactggat
     3061 cgaggacccg cccggccgcg aggtctttgc cgaaaccgct ggcaagacca cgttctccgc
     3121 ccgcaagatc atcgtcgaca agctgcgcga gtccggcgat ctggacggcg agccgaccc
//
LOCUS       DAWBMW010000183         3095 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_6405_length_3095_cov_1.975329, whole genome shotgun sequence.
ACCESSION   DAWBMW010000183 DAWBMW010000000
VERSION     DAWBMW010000183.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3095)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6405_length_3095_cov_1.975329"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    526..535
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1804..1813
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2376..2385
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          596 a          983 c          901 g          585 t
ORIGIN      
        1 cccggccacc gccatcgcca tgggcaagat gggtgcgctc ggcgtgctcg atctggaagg
       61 tctggggacc cgttacgagg atccgacccc gctgttggac gaaatcgccg gactgccggc
      121 cgatcagccc accgagcgta tccagcagat ctatgccgag ccggtcaagc cggagctcat
      181 cacgcaacgt ctgcacgaga ttcgcgccgc cggtgtcacc gtggccggcg cgctctcccc
      241 gcagcgcacc cagcagttct actccaccgt ggtggacgcg ggcgtggacc tgttcgtgat
      301 ccgcggcact gtggtctccg ccgagcacgt ctcctccggc caggaaccgc tgaacctcaa
      361 gaagttcatc tacgaccttg acgtgcctgt catcgtcggc ggcgcatcca actacaccgc
      421 tgccctgcac ctgatgcgca ccggcgcggc cggcgtgctc gtcggcttcg gcggcggcgc
      481 ggtctccgcc acccgccaga ccatcggcgt gcaggccccg atggcnnnnn nnnnnccgct
      541 atcgccgacg tggccgaggc ccgccgcgac tacatggacg aatccggcgg ccgctacgtg
      601 caggtcatcg ccgacggcgg catgggcgac tccggcagct tcgtcaaggc cctggccctc
      661 ggcgccgacg cggtcatgct cggcgcgccg ctggcccgcg ccaccgaggc ccccggcaag
      721 ggcacccact ggggttctga agcccgccac cagacgctgc cgcgtggcta ccgcaccacg
      781 gtcggcacgg tcggcccgct cgaacaggtg ctgttcggac cgagccacca ggcggacggc
      841 aagaccaact tcatcggcgc cctcaagcgc gcgatggcct ccaccggcta cgtcgacgtg
      901 aagaacttcc agcgttgcgg catggtggtc aacccgtatt ccgcacgata atcccgattg
      961 ccccctgaaa atgggggttt gccctccatg caggaggctt aacttgcccg aaggcgcgct
     1021 caagtagttt gacttggcgc gcctttcgtg atgttgtagt acagacgctc gccgatgatg
     1081 gtgagttgca agggggcttg aagggggtca tcatggagta ttcgggattt ttccacggca
     1141 catatcagta cgggccgttg ccgagttatg acggattcat cgttgtggag ctgtcggatc
     1201 gcccggtgcg gtatcacatc caacccacat tcataggcgg gctgaatgcg aaggaatgtc
     1261 attccatggc catggctgca tgccgcatcg cctgcacatc ggcggacgcg atacgtggca
     1321 aactcagcgt cgcacatctc aacagagcca tgacaaaacc atgcctggac cggctgcaaa
     1381 ccatgcaata cctgctcggc atccacatgg tcactcatcc ggagctcaaa gcgaaattct
     1441 gctatctgcc cactgtcccc acattgatcg acggcatgat caccggcaag gacacgctgg
     1501 aaatggcggt attcatgacc atcggccagg aaaatctgcg agtcaacctc aaactgaagt
     1561 acatcggttc tcgatggatg tgcatctatg ccgatttggg ctgaccagac ggccccgccc
     1621 gcacaccgat tcctacgctg gtagcggaag acggttctgc cgtgtcatag gctggttcgc
     1681 ctatagtagt gaccatgcgt gaacatgtag aagaacccag atatgtcacc accgatgacg
     1741 acaccgtcta ctcgctgctg tccaaacgag cagctcgcac gccggatgac atcatcgcgg
     1801 aatnnnnnnn nnntgacaac acccaccaat ggcgcagcgc cacagctact gaaatgctgt
     1861 cccgcgtacg cgaggtggcc aagggcctgt tgggacttgg cgtcaaagcc ggcaccatgg
     1921 tgctgattta ctcgcctacc tgctatgagt ggggtgtggc tgatttcgct tgcgcggcaa
     1981 tcggcgctgt ggttgtgccc gtctatgaga ccgattccgc ccgtcagacc gcgggcatcg
     2041 tcgaggaagt cgatcccgcc atcgcctttg ccggcgactt ccagcgcgca cagacgcttg
     2101 agcagatccg tgtcaatcat ccgggcatga aatacgtgtt caatttcaag gccgatggcc
     2161 ttgacgctgt cgttgacttc gggcatggcg tatccgatga gacgttggat gcggctatcg
     2221 ctcgtatcaa agccgatgat ctgctcaccg tggtctacac ctccggttcc accggcaagc
     2281 ccaagggcgc gatgctctcc aaccgcaatt acacgcacat cgtgcttaac ggctacgaga
     2341 ttctgcgtgg catgctgtac gacgacaacc gtctgnnnnn nnnnncttcg cccgctacat
     2401 ccagtacgtg tgcatcggtg gctgcggcgt ggtcggatac attcccgacg ccaagcgact
     2461 gctcgccgat atccgcagct tcaagcccac ctacctgctg ggcgtgccac gtgtgttcga
     2521 gaaggtgtat aacgcggcct cgcagaaggc tggtgccggc atccaggggc atatcttcgc
     2581 ccagtcggtc aagcacttcg tcaagtggag caaggatgag caggccggtc gcggccattc
     2641 gttcatcgag cgcatgcgcc attcgttcta catggccacg gtgggcaaat ccattcgttc
     2701 cgctctcgga ccgaacctca aatggctggc ctgcggtggt gcgccgctca acgtggattt
     2761 ggcccacttt ttcaacggca tgaatgacat caccttcatc cagggttacg gcatgaccga
     2821 aaccgccgct ccgatgctcg tcaattggga ggacgacaac gagatcggtt cggtcggcaa
     2881 gcctgggcct ggcatgggcg tgcgtcttgg cgaagacgat gaaattgagc tgaccggccc
     2941 gaacgtgttc cttggctact acaagcagcc cgaactcacc gcacagacga tgactgccga
     3001 cggctggatc aaaaccggcg atctgggtcg tatcgacgac cgcggcttca ccttcattac
     3061 cggacgaaag aaggacatca tcatcaccgc cggcg
//
LOCUS       DAWBMW010000184         3091 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_6414_length_3091_cov_2.700593, whole genome shotgun sequence.
ACCESSION   DAWBMW010000184 DAWBMW010000000
VERSION     DAWBMW010000184.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3091)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6414_length_3091_cov_2.700593"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2122..2131
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          657 a          931 c          909 g          584 t
ORIGIN      
        1 ggcctggccc acgatccaaa tggcgacgca catcagcacc atggcaacat aacgatcggt
       61 gacctgcaat gcgatgacga tcatgcacca ggcgagaatc gcgaagaaca gcaatacgat
      121 ggcgcaaccg acgaatccgg tctcctcgcc gatgatcgcg aaaatgaaat cgttatgggc
      181 ggccggcagg tagttccatt tctcccgcga attgccgatg cccaatccca gaaaacctcc
      241 ggaggcgatg gcgtacttgg cgtgaatgga ctggtagcat acgctctgcg cgtcagcggc
      301 cgagcaatcg ccatatgtgg ccagaatacg acgcaagcgg ttcgggctgg aaaccgccaa
      361 tgcaccgacc ataaccaccg cgcccaatac gccgactccc atccatttgc cggggaaccc
      421 gacgatcaga aaggccacgc ctccgatgaa gaccagaatc atggccgtgc cgaggtccct
      481 gccgcccatc accagcgcca cgcctatcgc atacaacacc aacggcgcgg catatgcttt
      541 gattcccttc ttgtggtaca tcttgctgca ggcatgcaac gacgagggca gccatatgca
      601 catcgcgaac ttcatgaatt cagccggctg gatagtggtg aagccgaggt tcagccaacc
      661 tttgttgcca tagacgtcga tacccaaagg agtaaaggtc agcgcctgca acaggcaggc
      721 acccaccaca aagagcacgc cggtccgttt ccagaacgtc accggcatca tcaatgccac
      781 gaagcccagc accaagccga tcaggcaaaa cgcgccctgg ttcagaagct gaaggaacgg
      841 cgacttgccc agtgcggcca tggtgaccgt ggagctggag aacaccatga tcaggccgaa
      901 gcatgtcagc cccaccacgg ccatgcggaa gccgtggtag caccatagcg ggttgagcaa
      961 gcttcgccaa cccgtgtagt cagtgatttc gagtgcgggc ttgtcaggga ctctgtatgc
     1021 cttacgctta tgggtatcag tggaggccat gcacctcact ccacgtcttg gctgcggcgg
     1081 cgaatcgatt gcctcgatcc gcgtaggact tgaactggtc catggaggcg caggccggag
     1141 ccataagcac gatgtcgccg gcggcggtat acgtgccgca ggcttcgacg gcacgatcca
     1201 tgacggtatc gttgtcttcg ggatcgatga tggtcaccgg gatgtccgga gcttggctgg
     1261 caaacgcttc gatcatcggc tgctggtctt tgccgatgat gacggccgct ttgatggtgt
     1321 gtgcctggtc cttgaccagg tcctcgaatc gactgccctt ggccagacct ccggcaatcc
     1381 agatcacaga cttggccggg aagctggaca gcgaggcgcg tgccgcatgg ccgttggtgg
     1441 ctttggaatc gtccacaaaa cgaacagagc cgccctcgac tgcggcctca gccacggttt
     1501 cgatgcgatg gccgcccggc ttgaacgagg taagcgcctt caacgcggta tcgcgatcag
     1561 cgccaagtcc cagcaccaac gccaatgccg tcaatgcatc ggccaccaga tgcgggtaca
     1621 gtgagccgtc cggctctgcc agatgcgtga aatcggtgat ggcggccagt ctgaccgact
     1681 ctcctactgc gccgccggcc acgccgctcc tgtccacgat ccagccatct tcaatgccga
     1741 tctggccggc ttgaggggcc tcgagcgtga atcccacctt gcggcatcct tcggcagtct
     1801 gagcctcggc cgccagttcg ctgactttgg cgtcctgcgc attgtagacg atggcgcgtt
     1861 tggcgttgtg gaacaccttg gatttgtcgg cggcgtaatt ctcgcgcccg ccatgccagt
     1921 cgaggtgatc gtcggcgata ttggtgatcg ccgcgcagtc aagtgccagc gaatcggtga
     1981 agtgcaattg gaaggagctc agttccacgc acagtacgtc gtgctgcgga ttggtggcgc
     2041 aacgagacag gctcatgctc atgtctccgg aggcgatatt gccggcggtc ggcgcgtcga
     2101 gtccgcaggc ggtgagcatt tnnnnnnnnn nccgaggtca tctcggtggt ggaggttttg
     2161 ccgttggtgc cggtgatgcc gatccatgga gccggagtac cggtgcgctc gttattcaca
     2221 cgcagctgcc atgcgaattc cacctcgctc atcacgggga tgccacggcg ttgggcttcc
     2281 agcacgaacg gggtgcgggg gttgaacacc ggggacgaca tgacgtagtc cacgtggtcc
     2341 cagtccacgt catcaaagga gtggagatcg gcctcgggct tcctctcgtc gacgccaatc
     2401 acgtgggtgc cgcgttccct caatacctcc gccaacgagg tgccggacac ccccagtccg
     2461 gcgatcacca ctgtcttatc cgctacttgc attgttcctc ctgatatagg aattccgtgg
     2521 gtgagcataa tccacccaga tttccgctag ctgaacagca ggccggagcg ggtgacccag
     2581 tcgccgtaga aaatcgtcag ggcgagcagc acgaagatga gttcgatcat ccagaaccgc
     2641 accaccacct tggtttcggt ccaaccctcc agttcgaaat gatggtgaat cggcgccatt
     2701 ttgaatacac gtttgtgggt catcttgaag tacccgacct ggatgacgtc gctcatggct
     2761 tccatgacgt acagtccgcc cagaaccacc gccaggaatt cggtgtgcgt ggcgatggac
     2821 agggctgcga acagaccacc caaggccagg gaaccggtat cacccatgaa aatcgatgcg
     2881 gggttcgagt tgtaccacag gaagccgaag caggcgacgg ccgcgcatac ggcgatgacg
     2941 gtcagatcga gaggatcgga cacggcatag gaatatccgc catggctgcc gcccttgatg
     3001 tggtagcttt cccagaatgc gatgatgcca aaaccagtga aggcgatcat cgacgaacct
     3061 gccgccagac cgtcaagtcc atccgtcaaa t
//
LOCUS       DAWBMW010000185         3081 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_6443_length_3081_cov_1.878057, whole genome shotgun sequence.
ACCESSION   DAWBMW010000185 DAWBMW010000000
VERSION     DAWBMW010000185.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6443_length_3081_cov_1.878057"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1893..1902
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          482 a          919 c          990 g          680 t
ORIGIN      
        1 gcggccagct cgtcgaaata gctggagcgc acgtcgatga cggcggcaag cttgtggacg
       61 cctgactccg atccggaacc ggaggtggtc atcatgtcga caagcgggat aagcagtttg
      121 ggaggcaggt tgtcgaccac ataccatccc atgtcttcca cgcagtcagc ggcatgagag
      181 cggcctgcgc cggacatacc ggtgatgagc agcacttcga aggcatcaag cggggtcggc
      241 tggttgtccg gagtcgatgt ggatgtcgcg gaattcgccg gcgcattcgt ggcggccgcc
      301 tcgccggtat cgcggttcgt tgtctgttga ttcattacag cgcctcctca agagctttgc
      361 gcatcgcgat ctccagctgg tcgtcttcgg tggtggtgtc ctgcagacgc tggtcggcat
      421 cgctgggcgg gtcgtcatcc cagataccgg tcatcagcaa tacctgtacg agagcttggt
      481 acagcagcat ttcttcgccg ccgatggcgt gaccgccatg agcgcgccag gcttccatga
      541 gcttggtggg gcgtggatcg tagatcacgt ccaacagcag tccggagaat ggctcggttc
      601 cggcatcggc gagggcatcc gcgacgttgt cggccgcgtg tccgggaatc gtgttgatga
      661 cataggtggc gttgcgggct gcctcgagca gggcctgatc gtcgcctaat tcgatttcgt
      721 tgtacgggtt atgcacgttg acgaactttt cggccactgg tttaagcccg gtgttttttc
      781 cggggtggcg ggcggcgacg acgatatggc cgatttcggg catcatgcag catgcggcca
      841 acgccgaagt ggcggtgttg ccattgccga tgatcagcgc ggtgccgctt ctcgacgcgg
      901 tgtggtgttc gccgagttcg cgattggcat ggtcgaaggc gagttggatg ccgatgacat
      961 cggtgttgta tagcttgatg cccggcttgc cgttcggcca agcgggatca tcggcagcca
     1021 cgctccagtc gaataccgcg gtattcgcca ccttcagttc cttggcccac aaattttccg
     1081 gaacaccgta cggttggatg gtcttcttca acggcatggt caggctcagg ccagcccacg
     1141 tcgagtcaag gcctttgagg aaagcgtcga ggtcgtcttc ccccacctcg tgcttgtcgt
     1201 acagccaatc gtcgagtttc agcgaacggt aggccgcgtt gtgaagcacg ggtgacaatg
     1261 agtgtgcgac gggtttgccg agtacagcgc aacgatgatt ggccatatcc cctccttgat
     1321 gtctgttccc atgctagccg acactttgct tcgaatatgt caaccacagg ccccgcaacc
     1381 gaaaatttac tctgggctgc tggaccgggc cggctcactg ctcgtgcagc gcgttgtaca
     1441 gcgcttcggc tttggcgtgg ccgatgcctt tgaccttctc gaagtcctcg acactggctt
     1501 cgcgcatggc tttgaccgag ccgaagtggt tgagcagccg cttctggtag ctttcgccga
     1561 tgccgggaat ctcgtcgagc gcgctgcgca gggcgccttt gcgccgctgc tggcggtggt
     1621 aggtgatggc gaagcggtgg gattcgtcgc gcacacgctg cagcagatac atgccttcgg
     1681 actggcgttt gaggatgatc ggatagtcgt cgtccggcac ccacacctct tccaagcgtt
     1741 tggccagtcc gcagacggcc acgtcgttca cgccacagtc ctcaagcgcc ttggcggcgg
     1801 ccatgacctg cggcttgccg ccatcgacca ccaccaggtt cggcttgtag gcaaagtgat
     1861 gccggtttgt gttctgctgg acgatgtcgg gcnnnnnnnn nnctcccgcg catcgttctg
     1921 cactccgacc ggcaccgcgt gccccttgcc actgatgtcg gaaccgccct caccattgtc
     1981 gttaccgttc gcagcgattg tctccgccgc cacggcagtg gtcatcttgc cggcggccga
     2041 ggccacacgc tgttccgcgt cgatgctttc gccggagtcg ccggcgatgt tgccgtgctt
     2101 gaagcggcgg gtgagcgtct cgtaaagcgc ggacagatca tccaccgcgc ctttgccgtc
     2161 cttgcctcgg atggcgaagc gtcggtattc ggatttcttg gcgatcgcgt cgtcgaacac
     2221 caccatggag gccacctgga aggcaccacc cacagtgttc gaaatgtcgt agcattcgat
     2281 gcgcagcggg gcttccgcga gtcccaatgc cttggccaca tcgttcatgg cctgggtgcg
     2341 tgcgcccatg tcggagatgc ggctcatctt gctgcgttgc agtgcttggc tggcgttctc
     2401 gttggcgcgg tccataagct gcttcttgtc gcctcgcgag gccacacgga tggtcaccgc
     2461 tcccccgcgc agattggtca gccagccttc gagttcctca cggcgggccg gttcgacggg
     2521 cacgatgatt tcgcgcggta ccggtgcgat gggtgccagc aggtccgcgc gcccggtggt
     2581 ctcctgacgg gtgttgcgtt ctcgggtggc ctgggcgcgt gccacagcgt ccgtggccgt
     2641 gatggtttgc gtggagccga tggcctcgcg attggtggag atggtcgcgg ccgactgggg
     2701 gtgattatcc ccggcggcgt ccgaatacac ctgcaccaga aggtcggcca tcaagtcggc
     2761 gtcatcgatg tcctcaactc gttcgacgct ccagttgcgt tcgccgcgaa tcgagccggc
     2821 gcgcacataa aaagcgtgca cggaggcttc gagctcgtcg ctggcgaagc cgaacacgtc
     2881 agcgtccaca tcctggtcga agacgacggc gttctgttgc accacggttt ccagcatttg
     2941 gatctggtcg cgcagtcggg cggctttctc gaattcgagt tcggcgctgg cctctttcat
     3001 gtcgcgggtc aactgggcga tgtatggccg tccgagtcgt ccggtcatca cgccgaccag
     3061 ctgttcgcac agccgccggt g
//
LOCUS       DAWBMW010000186         3059 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_6506_length_3059_cov_2.252663, whole genome shotgun sequence.
ACCESSION   DAWBMW010000186 DAWBMW010000000
VERSION     DAWBMW010000186.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6506_length_3059_cov_2.252663"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2826..2847
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          553 a          852 c         1010 g          622 t
ORIGIN      
        1 acacactggt gaaacgcacc cagtcccaca actgcttcag atagccgacg gtttgggtct
       61 gcgcgtcgat gacggaaacg atgaaacgac ggttgtccgt ggtatccaga tcgtagatgc
      121 gcgagccttc ggccagatcg tcgtccagcg tgacggccag tgaaccgtgg ccgacgccgg
      181 gctcctgatg ccgaatcagc gaggcggtgt ccagtccgat gcctttcagg gcggcgacca
      241 gatcttggcc ccacacgccc ttgttctgcg tgccgaccac gaatcggatc agcatgccca
      301 ccgtgcgacc ggcggccatc gaaaccagca tgccggtaac cgaatgccac gaaagcacca
      361 ccatcaccgc ggcgatggca tacaggatat tccaactcca tttgaccgtg gagcgggtac
      421 ggcgtggccc ggcggcggtc aggaaggacg ccatgccggc gtagatgtcg ggcaacagcc
      481 cggagccgta tgaggtggcg gctgagacca gtgccattgg cagggtgaaa tcgttcagac
      541 tgctgattgc cgtcgatatg acccacacaa tgccgtatcc ggcaaacatg gccagcgcgg
      601 acaccgcagc ttgcagccat tcgcgggcca agagcaactg agcgagcacg ataactacga
      661 tgaccgtggt ggccagctgc gtcagtacgg tggaggggaa agcagccagc cagttgatgg
      721 cttgggcggc ggtatgcgcg tcggattcga cgccgcgcgt catgccgccc agatacacgg
      781 cgaacaccat gaccacggcg gccatcaata gcgacagacc ggctcgggtc aggtcgccga
      841 agtcgcgcgt gcggcgcgga gcgatgtcgt cgatgtatgg tccggaatcg ttgctgtcgt
      901 cggttgccgg gctgtccggt ccgctgacag caaaactggc cgaccggccg tcaccagagg
      961 cgatcggccg gtcggtagtg gaggaggtcg ccttgttcgg gcgatcttcc gtagtggact
     1021 gatcctgttc agtagtcatg aaatggttac ctgacgcctg tccttgttcg gttcagtttg
     1081 cggttcggtt tgtgactcag cgcagtagtg tggtggatgc gagggattcg aggggctcga
     1141 gggactcaag agactcgaga gactcgcatc gcgcctatct cagcggccga aagcgacgag
     1201 ctggctggcc acaccggtgt aggtggcggg ggtcagagcc ttcaagcgag cggcggtttc
     1261 cgggtcgaag gactgctggt cgatgaactg ctcgacctgt tccttgttga tggtgtggcc
     1321 gcgcataagc tccttgacct tctcgtacgg cttatccatg cccggcaggc ccttgagctc
     1381 gcaggcgcgc atggcggtct ggatcggctc gccaagcacc tcccagttct catccagctc
     1441 acgctcgatg acgtgggtgt tcgggtggat ggactcaagg ccacccatga ggttggtcag
     1501 ggcgagcaag gagtagccga gcgcggaacc gatgttgcgc tgggtggtcg aatcggtcag
     1561 atcgcgctgc caacgggatt ccaccagcgt ggcggagagg gtgtcgagca gcgagcagct
     1621 gatctcgaag ttggcttcgg cgttctcgaa acgaatcggg ttgaccttgt gcggcatggt
     1681 ggaggaacca gtggcaccct tgaccggcac ctgggcgaac acgccgcggg agatgtacat
     1741 ccacacgtcc acgcacaggt tgtgcatgat gcggttggcg tgggagacgg tggagtacag
     1801 ctcggcctgc cagtcgtggc tttcgatctg cgtggtcagc gggttccagg tcaggcccat
     1861 gcggttcgtg acgaactcgc gggagacagc gatccagtcg acatccgggc atgcggccaa
     1921 gtgggcaccg aacgtgccgg tcgcgccgtt gatcttgccc aggtactcct ggttttcgat
     1981 gtgcttgagc tgacggttca gacggtagac gtagacggcg agctccttgc cgagtgtggt
     2041 cggcgtggcc ggctggccgt gggtgagaga gagcagcggc atatccttga attcctcggc
     2101 cttgccggcg aggtagtcga tgatgcgctt gaactccggg gtccacacgt tttccatcgc
     2161 gttcttgacg caacgggcga tggagaggtt gttgatgtct tcggacgtgc aggcgaagtg
     2221 cacgagggtc ttcagctcgt cgttgatgtt ggtcagctcg gccggagcct tgtcgatctg
     2281 atcatcgatg tagtactcca cggccttcac atcgtggtgg gtgatggcct catgagcggc
     2341 gtgctgcttg atgccttcgg cgccgaagtc ctcgggaatg gcgcgcagga aggcctgctc
     2401 ttcggcagtg aacggcttga cgccgtcaac gatcggctgg ttgccgttgc cctcaaagcc
     2461 gttggccaga agaatcatcc attcgacttc gacacgcatg cgttcgcggt tcagggcagg
     2521 ttcgctcatg tattcgacga gcggggcggt ggccttgtga taacggccgt cgagcggggt
     2581 caatgcgatt gcgggggaaa tatcggtaag cttcataacc tccaagggta cttccagccg
     2641 tgaaccgagc ggctatactg tggtgcggcg ttcccgcccg agccgcacag gtcaaggaac
     2701 gcccgtggtt cgattactgt gtgcggcctt taaggctagg agatcaccat gcctgcaacc
     2761 accattcata ctcctccgaa acgtccgatt gccgtatcgt cactgaaatg gcagccgttg
     2821 gatatnnnnn nnnnnnnnnn nnnnnnngcg tgatcttctg gggattcaac ttcgcgtaca
     2881 tgccgatctc ttcggtattc ggcgcgattc tgcccggttt cgccagcatc gtgcacgccg
     2941 tctggtactt ctccggtacg ctggcggtgc tgattctgcg caaacccgga gcggcgctgt
     3001 acgtgaacct tatcggcacg ctggtcgaaa cggtgattgg tagccagttc gatttgggc
//
LOCUS       DAWBMW010000187         3040 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_6560_length_3040_cov_2.476382, whole genome shotgun sequence.
ACCESSION   DAWBMW010000187 DAWBMW010000000
VERSION     DAWBMW010000187.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3040)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6560_length_3040_cov_2.476382"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    966..975
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1368..1377
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          560 a          823 c         1017 g          620 t
ORIGIN      
        1 ggatgtgacg gtgcaccacg aactgacggg cctgggcggt ggtgcgggcg aagccggcac
       61 gcagcaccag gttgtcgaga cgggtctcga ggtcggtcag catggcgtta ccggtctggc
      121 cggcagtacg ggtggccttc tcgtaagcgg cgcgaagctg cttctcggag atgccgtact
      181 gagcgcgcag acgctgcttt tcgcgcatac gcaccgcgta atcggactcg gtgcggcggc
      241 ggtcacggcc gtgctcgcca ggagcgtacg gacgcttttc gaagatgcgc tgagccttgg
      301 gggtcagtgc gatgccgagg gcgcgagaca gacgcacctg acggcgagaa cgctgaacgt
      361 tcgtcattgg attaatcctt ctgtcgttat ctgaacggaa cgcttgcctt gcatatgcct
      421 aacaagcgct gagtcgggcc tccccagcgc ttcgtcgtga ataaacacga acgggttggt
      481 tttcttgcga ttacctaccg ccgacccgct gatgggctat gactccagac ggtatctacc
      541 tgttggcaga caccagcaga ccatattaca ccgaggctat ggacagcact ccggcgtcca
      601 tgcgatacac ccgatctgcg tattcgagtg catcgggatc atgggtgacc atgagaatcg
      661 ccgtgccgtt gttggcttgg tctctgagca gacgcattac gatgtcggtg ctctcttggt
      721 cgaggtcgcc ggtcggctcg tcggcgatga gcaagctcgg ctggttcatc agcgcgcggg
      781 cgatggagac gcgccgcatt tcaccgccgg acagctcgcg cgggtagctg tctgccagat
      841 cggcaacacc aagctgggtg aacagcgtgc gagctcgggt ggcgaacagg tccgaacggc
      901 tcgcagccgt gtttgacggc gtgttttcct ggtctgtgat tggcgcaatg acatccggca
      961 tgaacnnnnn nnnnntcatc attggtggca gcggcttgcg ccacggcatc cgttgcttct
     1021 gcgttttggg cgaacggcag cggctccgga aacatcgttg cgggcagaat cacgttgtcg
     1081 agcacggtca ggttgccgag cagggtctgg ctttgagtca cgaatccgat ggtgcgattc
     1141 ctcagcacgg acagtgcttt atcgcccaag tcggccacat tgcgtccagc cacgctcacg
     1201 gttcccgagg tcgggcgaat cagcccagcg accatgttga tcaacgtact tttaccgttg
     1261 cccgaccggc cgacgatggc gatgaattcg ccggcatcga cggtgaggtt cacgtggctg
     1321 acggcgtcga acggcgtgcc gcggcggacg aactggcggg tcaggtcnnn nnnnnnncgt
     1381 caaggtcagc agatggtccg gagagggcgt ctcggttacg tgcggcgaat ccggcgcagt
     1441 cggcgcgttc ggctgagctg gcgtggtcgg ctggagcttg gtcggctcaa ccgtggtcgg
     1501 ctgaagcggc gtagttgtct cagtcatatc actccccctc tcgcagggtc aggtaggctt
     1561 cgggccggcc gagacgccac atggtggcca gcgaaccgat gatgccgatg accgtagagc
     1621 acacaatggt cacggcaatg aatccgatca ccgcaaccgg gctggcttgc agatacggca
     1681 attgaagctg tttgccgatc agcgaactga atgggaaaat caccaggctg gccgcgccga
     1741 cgccgattac gccaccgatc agaccgatga tggcggattc cttcagaatg atggcattca
     1801 acatgccgcg cgtggcgccc atgatgcgca gggacgcgaa ttcgcgtttg cgttcgttca
     1861 ccgacgaggc gaatacggcg agcagcacaa tcacgcccat tacccagatc accgccacga
     1921 aaatcttgag gtaggtgaca agcgcggtca gcgaagttct ggtgttggcg gtgatgccgc
     1981 cggggtatac gtaccccaaa tcggcgaatc gggaatcaag acgcttgatg ttctcagcta
     2041 cggtctgcgc ttcgtacccc tgctgcgtct taatcatcac ggtggagacg gctttgccgg
     2101 catattcggc ggacatgacc tgcttggaga ccttggccga ggcggcgacc atgtcgggca
     2161 cggtgttttg gttgatgaac accgagttgt cgaggctggt gccggtgttg gcgagctggg
     2221 cgacgaccgg ccatgaacgg ccgtagagtt cgatggtgcc gtcggtggac acgttgacgt
     2281 ttgcgcctgc aatcatctga ccgtcttcaa gggggccttc gaactgggag gcgacccatg
     2341 gttcgatgac gaagtcagtg tccgggtcgt aaccgatgat ctgcagcttt tcggcacagc
     2401 aggcggcggc cagcgatgat atataggtct gttcggtggc ctgtgcgatg ccgtcggcct
     2461 gcatgacctc gtcggcgatg tctttggtga aatagaaagt gctgggattg ccgttggtga
     2521 gcagcgcttc ggccttctgc acggtgtttt gcggcacgac catgaggtcg gcgcccaggc
     2581 gcttttccat actggtcatg ccagtgttga ggttcatggt cagcatggtg ccgccgaaga
     2641 atgtcagcgc gaggatggtg acgacggcga gcagtgcgcc ggtgcggaac ggcttgcgtt
     2701 tgaggttttc aatcggaagg ccgcgcaatg cgatatggga tggcacgatg aatccttatg
     2761 ttgcttatag tgcggttgca ataagcgagg ccggagtcca gcgttcggaa tggctgggct
     2821 ccggccgtct gctcgatcag ttgcgcttgg cggcgcgaga gtcgagccag atggcgatgg
     2881 ccgcgaacac gatggtgagc acgccgagca cgatgagcgt gggcagcata accatgcgac
     2941 agtgcatcat cgcgcccttg cagatgccga tcagaaaggt gggtacggcg agtagtagca
     3001 gggcgtcggc tgcggcggcg atattgaggc cgatgcgaat
//
LOCUS       DAWBMW010000188         3038 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_6567_length_3038_cov_2.570567, whole genome shotgun sequence.
ACCESSION   DAWBMW010000188 DAWBMW010000000
VERSION     DAWBMW010000188.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3038)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6567_length_3038_cov_2.570567"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    558..567
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          709 a          920 c          837 g          562 t
ORIGIN      
        1 aagccgttaa taccaatctt aactgtcatg taattctccc ttgtagggtg gccttggcgc
       61 gctttggcac accgggccat tgtttaccaa cagttgctac tgtaatgccg actctgtaca
      121 catggcaacg tttcagcgtg cgttttgggt aaaaatgccg tattttttgt gagcgctcac
      181 aatttggatt gcccgcaacc gacatgtgat ttttcctctc ctttggagga atgtctcgaa
      241 tcatcatgag caagcgcgcg gcatgcaagt cagcgaacca gcagcttgga cactgcggtg
      301 tccagctcgc cgatatcgcc gccaaattcg tggaatcgac tcacctgtgg caacgccact
      361 tcgatgggga aggtgacgat cagtgtgccg cgtttcacgc tgatggccgc gtccacgccg
      421 tcgcggaact cgttgcttac cccctgcacc accgtattgg tcagcgtgtc gtctttaagc
      481 tcatatttca gccccggttc gtccacgcca agcgaaacat cggagtggga gaacgcagac
      541 accatgcggc cgtcctcnnn nnnnnnnaac gaaagcctac catcggtgat ggccgtgacg
      601 atgagcccat caccatacag atacccgctt gccccatgcc gggccaataa tgccatcagt
      661 tgaatgccgg aaatcgtatg gtcaatgcgg ccgcccaaac cgccatagac atggaattcg
      721 cggcaacccg ccgaccaacc gaccttcagc gcggagagca tgtccggatc gtccttcagc
      781 gcgggcagcg cgatggtgcg cacatcggtg cggggcggcc ggccctccag cgaatcgaag
      841 tcgcccacca ccacatcggg cacgataccc aactggcgcg tatggtcgag cccgccatcg
      901 gccgcaatca catacgcgcc ggccggcacc acgggcgtgc ccgcatagta ttcgcccgca
      961 ccaaaaatca cgcatacctg agtcatgatg cctactgtag ccggtatcca cccatcggcc
     1021 gggaaaatgc tgataggcta cgcatcagaa cagtagtcga taggcgcaca ggcaggggag
     1081 cggagcgatg gcacagatca ccgaggcaag cgaacggccg attctcaaat caaagatatt
     1141 cctctacatc accgaattct tctccggcat gtccgctgat ctacttcatc tcgatcaagg
     1201 ccaagcgcat cgcctgcgtg gtcagcgccg tgatcttcgt ggccacgtcc tgcgtctcgc
     1261 ccctgaccgg cttcgcgttc tgggaaacca acctggccta tgagggcgag tccatctaca
     1321 actacctgca ggtcaagaac ctctccgacc gcacgatcct gtccaccaac gtgctgttcg
     1381 gcgtgcagtc cgtcacgatg aaagacaaag gactgacagg tatgtactac gacacggccc
     1441 tggctgcgcc ggcgctcgcc gacaatgcca attccgcgct gatcctcggc atgggcaccg
     1501 gcacctacgc ccgccagctg aagcagtatt atccgaaaat gaacatcacc ggcgtggaga
     1561 ttgaccagaa gatcaccgat ctggccggcg aatacttcga tgagcccgcc gacatccccg
     1621 tgaccacgta tgacggccga gcctggctcg ccgcctcgca tgacaagtac gacgtgatca
     1681 tggtcgatgc gtatcaggac atcaccattc ccttccagat gagctccacc gagttcttca
     1741 ccatggtcag agagcacctg aaccccggcg gcgtgatggt ggtgaacatg aacatgatct
     1801 cggacggcca aggttcgatc aacgaggcgt tgagcgatac catcgccagc gtgttcggca
     1861 atgggaacac actgacggcc gacgtgccga acaccacgaa ccgtgaactg ttcgccaaga
     1921 agccgggttc cggctccgaa gagaactcca tgcagcaggc ctccaaggcg ctcaacctgc
     1981 gtgagaccac ctacgagcgc accggcagcg aagatctcga atggtatatg gaagaggtcg
     2041 cctctcgatt ccggaaggtg aacgaaccgg actcagcgtc gactattctc accgacgaca
     2101 aggcgccggt cgaagtactc ggcatgcatg ccatcgatca aatcatcgcc gatgaggccg
     2161 gcccatatcg gcagattctc aaagacgaag gcttcggcgg tctgctgcgc gccgtgcagt
     2221 aggttcgaca cacggaccgc gaatgtccgg gcgtatggta gactttctat ttggcttgag
     2281 aaatcaagaa agcggggcaa ccccacctgg aagcctttca aggcctcggg ttcaaggcaa
     2341 acagcctagc gattgcgata tacaacgcgt aatcaggaat gacgcaaaga actgataagc
     2401 tgagcgcata ttggcgcggg ctttggcttg ccatgaaccc aaagcattga acaggactcc
     2461 agcaaaggcc ctcgccacgg aattgaagta gaggattgga gtcatcatca gcgacgaacc
     2521 acgtattaac gacgagattc gcgtcccgca ggtacgcctg atcggcccga agggcgagca
     2581 ggtgggcgtc atcgcgactt ccgtcgcgct gaacctggcc aaggaggcaa acctcgatct
     2641 cgtcgaggtg gccccgaacg cgaagcctcc ggtcgccaag ctcattgatt acggcaagtt
     2701 caagtacaac gagaagatca aggctcgtga agctcgccgc aaccagagca ccgcggaaat
     2761 caaggagatt cgtttccgcc tgaagattga cgaccacgat ttcgacgtga agaagggcca
     2821 tgtgacccgc ttccttaacg gaggcgacaa ggtcaaggtc accatcatgt tgcgcggtcg
     2881 tgagatctcc cgcccgattg gtggcgtgga actgctgcag cgtctggccg acgatgtcga
     2941 ggaatacggc accgtcgagt ccaagccgaa gcaggagggc cgcaacatca tcatgaccct
     3001 tgcgccgaag ggcaagaagg tgcacaccca gtccgagc
//
LOCUS       DAWBMW010000189         3007 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_6637_length_3007_cov_2.020664, whole genome shotgun sequence.
ACCESSION   DAWBMW010000189 DAWBMW010000000
VERSION     DAWBMW010000189.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6637_length_3007_cov_2.020664"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          546 a          839 c          998 g          624 t
ORIGIN      
        1 tgacgccgag atgctccttg aaatatcggc ggccgaacgt gatctgccgc accaatgatt
       61 cgccggaagg aatcatgttg tcagattcca cccacatgcc gccgaccggg atgaaccggc
      121 cttcttcgat gcgccgcttc attcgtgcga acaggtcggg atgttcctct tccagccatg
      181 cgtactgttg ggcggaactc atcgcatacg tgaagtccgg gtcttcgtcc attaaagcca
      241 gcacgttgga tacggtacgg gccactttgc ggcgtgtctc gcgcaccggc catagccatg
      301 cggagtcgat atgcgcatgg ccgatggcaa tgtggttgat tgcgctggat gcagccggtt
      361 cggcgagcac gccggcgagc gccgcacggg ctgccggcac ggtttccaag tcgcgttcat
      421 cgtagatgtt caaggagcgt tgcagcgctt tggcgagctg ccagtagcgc gggtcgtcgt
      481 ctttgagttc gcaaatcagg gaggatacgg tttccaagtc catgtcatag gcgaacacgt
      541 cttcgttgaa gatggtgata tccatgcgac tcagtgtgta tgggaagtca caggtgccgg
      601 tggcctcttc gccgagttcg gtgggtgaga acggggtggg gccttcgacg aatgggttgg
      661 cggcggcttc gatatagacg gtgaagtcgc catgctcatc gagttcgaca gtgctgccac
      721 cgtccgcgta gacgagtgga atccagtggt tgcgtgggtt cgcggatttg atggcggtgc
      781 cgtcggcacg atagacaagt ccctcggatt ggaatccggg gccgcggtga tcgagccagc
      841 cgaggtctac catgagttcg acttttcgtc ctttgaccgc cgccatgtcg atgtggccgt
      901 tcacctcgaa ccatgtggtg ccccatgtgg tgccccagac gccgggaacg cggaaaggtt
      961 cgaagggcac ttggccgttg cgcactttgg ctagaaattc gctggagggc accggttcgc
     1021 cgggattgtg gaacgaacgc aaggtgcatg cggcgagtgt ggtgtggata tgcggtttga
     1081 cgcgctgctg catgacgcgg tcgcagcgct ccaactgctg attgggcaga agaaacatgg
     1141 agaattctcc tttgctgtgt ctgatgggaa tgatggaaat gatgggtgtg gtggcaattg
     1201 ggaaaaccgt aatacgacat gcttctgcct cctcatggtt cgaggaggca gaagcgtcag
     1261 acgggaattc agcgcgtgat gcgcagggtg gcgagctgga acgggccaaa gtgcaacgtg
     1321 gcaccttcgg cgtcctgcct accgtcttgc agcgcgactg gaagatcggc ggccagatcg
     1381 tcgccttcca gcacattggt ctcatgcacc gaagcgcctg cgagcgccgg gcagacgtgc
     1441 agcatggcat cagcctggcc accggccgcc tcataggcac gtacgatcag gtcgccggaa
     1501 ccatcatcgg ccagcttcat ccagtccagc accacggtac cattgacgga ctcaatcgag
     1561 gcgagcggcg tgatatcggg cacatcatgg agtaccggtg cgttgagcac accggcggcg
     1621 tcaagcacac ggtccaccgt ggcgtcggtc accacggacc agtcgaactc gtgcgagccg
     1681 atatcggtgc gcgggtcggg gaaggtcggc gcggagagca atgacagtcg gaacatggtg
     1741 cccgagtcac gaccttcggc ggcgttcccg gagatcggcg tggcatcgga cccgtatacc
     1801 gatccgttga tgacggccgc cgcgtaacca gcgtcgccga taagggcgaa gcggttcgtg
     1861 ctactctcat acttggcttc gtcggatgcg gtgtttttga cgattgggcg gcggaccaga
     1921 ctggcagtcg taggtcgcct gatcggccac aatgcccagc ggcaaggcga ccttgaggaa
     1981 acgttcgcgc tcatgccagt cgatatcggc gtggaaatcg agcgtgtgtg aaccggggcg
     2041 cagcgtgatg gtggtggtga tgacggtgtc atcgtcggcg gtgcgcacgc gaactcgtgc
     2101 cgcaccgttt tcggtatcga ccgattcgat tgatccggtc atggcattcg ccatgagcag
     2161 ggattcgcgt tcgatctccc aggcatccca aacggcgggt tcgtccttca acagttcgta
     2221 ttggcccaat cgggtgccgg ccggaaccag ttcacggcca tgctcctcgt caagcagcga
     2281 cgagattgta ccgtcggcct cgatggtgac ggacagtacg ccgttggcca gcagtacacg
     2341 gccgttgtcc aatgtctggg tcagtacgga aagcgcgtcg gtcggttcgt tgatgcggct
     2401 ggcacgccaa gacacgccgt cattgcggaa ctggctgatg cgggcctcgg cgagcaggtc
     2461 ggcctgcggg ttggccttgc gcagcacggc gcacatgtct tgggcgattt cggcgagacg
     2521 cttcagatcg cggcgatagt cctcgcgggc ttcgcggtgc acccatgcga tggacgaccc
     2581 cggcagcacg tcatggaact ggttgagcat cagggtcttc cagatacggt ccagctcttc
     2641 gcgcggatag gcgtagttcg ggtcggccaa cgtggcggcc gcgcccaggt attcgaccgt
     2701 gcgcagcagc gattcctctt ggcggcagcc gcgtttcatg tcctgctgcg aggtcaaagt
     2761 gccgcgatgc agttcgaggt acagctcgcc cttccagacg ggcatttcct ctccggcgtt
     2821 cttttcgatc tgtcggtggg ctttgtcgaa gaaatcgttc gggctctcaa tagttaccct
     2881 ggagacacct tcgaggttct cgtagcggtg caggtgctcc atcatgttgc gcgtggggcc
     2941 gccaccgccg tcgccgaaac cgaagagcag aagcgagcga tcggcgagat ccttgtcctg
     3001 gaagttc
//
LOCUS       DAWBMW010000190         3000 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_6659_length_3000_cov_2.781664, whole genome shotgun sequence.
ACCESSION   DAWBMW010000190 DAWBMW010000000
VERSION     DAWBMW010000190.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3000)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6659_length_3000_cov_2.781664"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          609 a          807 c          891 g          693 t
ORIGIN      
        1 tgaatcatgg cctcacgctg cttggagggg gcttccttcc agatgatctg gtcttcggaa
       61 tagccgagtt tcttggcgac gtatttggcc acgtccacgt cgaagcccac gtaggtgccg
      121 gacttcttgg agcccaggcc gggctggtcg aatttgatgc cgatgcggat cttgccggtt
      181 tcgtccgcgc cgcaggcggc cagcgacatt gtgcaggcca cggcggcgag cgcggcgatc
      241 acgcggcaag ccttccgttt gaacgtattg gtgaatgtca ttggattgcg tacccgtctt
      301 tcagtgggtt cgtcagtggg tgaggatctt cgagaggaag tccttggcac ggtcggtctg
      361 cggatgctcg aagaactcgt cgggcgtgtt ctcttcgaga atctggccgt ccgacatgaa
      421 gacgatgcgg tcggcggcct tgcgcgcgaa acccatctcg tgggtcacgc agatcatcgt
      481 catgccttca tgcgcgagtt cgaccatcac gtcgagcact tcgttgacca tttccgggtc
      541 gagtgcggag gtgggctcgt cgaacagcat gaccttgggg cgcatggcga gcgcgcgggc
      601 gatggccaca cgctgctgct ggccgccgga gagctgggag ggcatcttgc tggcctggga
      661 gtcgacgccc acgcgggcga gcaggtccat ggccagttgc tcggcttcct tcttgtccat
      721 gtgacgcacc ttgatgaggg ccagcgtcac gttttcaaga atggttttgt tggcgaacag
      781 gttgaacgac tggaatacca tgccaacttc ggccctgagg ttcgcgagct ccttgccttc
      841 ctgtggcagg ggcttgccgt cgatgcggat gtcgccggaa tcgatggtct cgaggcgatt
      901 gatggtgcgg cacatcgtgg acttgcctga gccggacggg cccacgacca ccagcacctc
      961 acccttggcg acggtcaggt tgatgtcctt gaggacgtgc aaagcaccga agtgcttttc
     1021 gacgtgggtc agttcgacta gcggacggtt cgggtcctct ttgccgggaa ccagcggggt
     1081 caatttggcc ttgctcggtg atgttgtgct tgtcgcgcgc gaacgcactg ctgtttcatt
     1141 gttcgtttca gacatgagtg ggaggatagc acaccgacgt ttccaacgtg ttcacagtat
     1201 gtccacacag ttgctcagtc gatgtctcat ttccgcatca gacatcgtga aggcggcatt
     1261 gggcatcggc atacattctc gctgagtcgc gcatcgctcg gcgtctgctg tgcgctgatt
     1321 gcctgctgcg tggtggctga gacgttgctg cgacggcaaa gcgggcacgt gttgtcctat
     1381 ctgctgattc catgtttggc cttggtgtcg ttggcgttgc tgccgtgtat gccgcagtcg
     1441 ggcgcatggc tggcggtcac tttgtattgt gtggggctgg tctcgccgtt tcccatgtcg
     1501 tattcgtata aatgcgctag gcattttgag cgtggctagc tgttcgcaca cgatgagtgc
     1561 tgttatacgg tggacgacac tcccgaagcg cggtggataa ccgggcgcgg cacacgatat
     1621 gccggtggat aaccggttcg cgccgcttaa gttatcccca tttttcggtt ttgcccctgt
     1681 gccaccgtgg aatgcgctac gctcggcgca tgaacggata cagcgaagtg ccgcacaatg
     1741 gcgaaatcaa caactatctg cgcaaagatc ttgacgccaa agccacggcc aagaacacgg
     1801 cctgttggaa tctgctgggc aaagtgcgca cgaaacgcac gttgtgtttc tcgctgtata
     1861 ccgcattgga attatgcggc gtggagttgc cgaggcacag cacgttgccg cagaaggatt
     1921 tttatgtgac ggtgaggagc aaggggaccc gctcttcatt ggacaatgtc gactatcgca
     1981 tatggaatgc aaagttcaac agtatcgctt tctcaaacgg cgtaacctgc atgcatccca
     2041 tggatgcgtg gatacagttt gcccaatatc tcaacctcac tgaattagtg gtgctggctg
     2101 aagcgctcat cagacgttat ggatatgcca ttgaacaatt cactcaacga ctaacggcct
     2161 ttcaccgcgt tattggccgt gcgcgttgtg aggcggcgct gaagctcgta aaaccatcgg
     2221 attccgttca ggaaactcgt acacgcttgg cgcttatgct tttcggcctg cccataccgc
     2281 aaacacaata tggcattacc gattcagaga acggatacac ctacaccgtt gacatggctt
     2341 atccacaata caaagtcgct attgaatatg atggcgatca tcaccgtcgc ttccgcaaac
     2401 aatatgtgcg ggaccagcaa aaacgtcgac gtctgcggca attgggctgg accgtcatcg
     2461 aggtgttcgc cgatgacctg tggaacaccg ccaaacaacg tgccttcgca caagaggttg
     2521 ccaccgcgat gcaaataccg cttcctggcc gtcctcaacc ttcatgccgg gtattaatag
     2581 acggcagcct taccatcaat gcgcgcaaag gggaatacag gaggcggaaa caagcaaaac
     2641 acaacaaagc atcacaacat tgatcaaata cggagttgcg atcgctcctc atggggcggt
     2701 ttgaagaaat tgtgaaaccg cggaattaag ccattttgac ctaccgcccc atggggtttt
     2761 caccgcccca tgggttgttt accgccccat gaggaagtta ccgccccatg gaaggataat
     2821 cggcaaacgc caagggggac accgcatggt gcgaggtttg ccgcatggcg gaggtgccat
     2881 cgggcggagt atttggcacc ccttggcagg tatttggcac attgggcccc acccgcaaaa
     2941 agcaaatggc ccttggaatt attccaaggg ccatttgcgg ctaatagcac cggagcatta
//
LOCUS       DAWBMW010000191         2996 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_6668_length_2996_cov_2.232574, whole genome shotgun sequence.
ACCESSION   DAWBMW010000191 DAWBMW010000000
VERSION     DAWBMW010000191.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6668_length_2996_cov_2.232574"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          591 a          987 c          788 g          630 t
ORIGIN      
        1 accgtgacca gctcatcgac aaggtgctca acggcaacgc caccgccgcc accgagttca
       61 cctccccgaa gaccccgggc tactccgact ccctcaaggg tgccgacaac ctgaagttca
      121 acgcctccaa ggccaaggaa ctgtgggcca aggctgacgc catctccaag tacgacggcc
      181 agctcacctt ctcctacaac gccgatggtg gtgccaagcc gctttacgac gccgtcgtga
      241 accagctcaa gaacaacttg ggcatcgacg cggtcaccaa ccgtcagatg aagggcgcct
      301 tccgtaccgg ctggcagcct gactacccgt ccgccgagaa ctacctgtgg cagctgtact
      361 ccaccgctgc tgctgatggc aacggctcca acgatggtga ctacaagaac ccggccttcg
      421 acgacatgtg caagcaggcc gctgccgcca agtccgtcga tgacgccaac aagctgtacc
      481 agcaggctga ggaaatcctc ctcaacgacc ttccggccat cccgctgtac tactccaacg
      541 cctccggtgt ggcttccctc aacgtgaagt ccggctacgc cttcgattgg cagaacctcc
      601 cgacctacac cgagatgtcc aagaagtgag tctgactcac tgaacggata ccgttaggta
      661 gttcggaaat aaagggcgag gatcgcaaga tccccgccct ttttgttgtg tgaggggtgc
      721 ttgctcccgc cagcgggaac caagttaaag tatccactat ccgaccgcaa ccatggacat
      781 atccaccagc attgcccaat ctcaaacctt gcgttaacgc aaatttacat atcacagtcc
      841 gggcatgcgg taaactgtcg gagtatttat cgaccggtgg gggcgtacta taccctttct
      901 ggccggtcta ggaaataagg agcaaccgta tgggtaagta tcttctgcga cgcattctgc
      961 agatgattcc cgtggttctc ggtacgactc ttctggtata tgcccttgta ttcgccctgc
     1021 cgggcgaccc cgtcaaggcc atgttcggag acaagcctgt caatgaggcc gtcgcagccc
     1081 agatccgcgc agaataccat ctcgaccagc cgttcatcgt tcagtacttt atctacctga
     1141 agaatgcact gaccctgaac ttcggcgata ccttcgccgg ccagcccgtt ctcgacgaaa
     1201 tcacccgcgc cttcccggtc accatcaggc taggcttgat ggcgttcgtg ttcgaggcga
     1261 ttttcggtgt ggtcttcggc attatctcgg gcctcaagaa gggcaagtgg tacgacaccg
     1321 ttatcctgat cgtctctctg ctgcttattt ccgtgccgac cttcgtcacc ggcttcgtca
     1381 tgcaatatgt ctttggtatt cagtgggcga tattgccggt gacggcgggc gccgaccccg
     1441 gcttccttga tctgctcatg ccggccatgg tgctcggttc ggtgtcgatg gcctatatca
     1501 tccgactgac ccgctccgaa atctcctcca acatcgccga ggattacgtg cgcaccgcac
     1561 gcgccaaggg catgtcgaac ggtcaggtca tgctgcgcca cgtgctgcgt aactccctga
     1621 ttccagtcgt cacttacctc ggccaggaca tcggcgccct gatgggtggt gccatgatca
     1681 ccgagcagat cttcaacatc cacggcatcg gcttcctcac ctaccagtcc attctgaagg
     1741 gcgaggcgaa cctggtagtg tccatcgtca cgttgctcat gctgattttc gtagtgtgca
     1801 acctcgttgt cgacatgctg tacgcggccc tcgatccgcg tatccgttac gcgtgatagg
     1861 gaggagtgag tcacatggca aacgaaacca ctgaaaacga gctcaaccag accggcgagc
     1921 caggaaccga atacttcatg gaaaccctgc ccggtcagga ccgttacgta gctcctctcg
     1981 acgagacccc gttgcaggac gtcgattccg tcgacgaatc cgctccggcc accagcatgt
     2041 gggccgacgc atggcgaacc ctgcgccgca acccgctgtt catcatctcc ggcttgctga
     2101 tcctgttcat catcgtggtg gccatcgccc ccggtctgtt caccaagcag gatccgaacg
     2161 cctgcgatct gggcaactcc ttgagccccg cctccgccgg acaccccttc ggctacgacc
     2221 tgcagggctg cgacgtgtac acccgcgtgg tctacggcac ccgtacctca ctgtctgtcg
     2281 gcgtgctctc cacgctgctg gtcgtgatcg tcggtaccct gatcggtgcc gtcgccggct
     2341 tcttcggcgg ctgggtcgac gcggtgctga gccgtatcac cgacatcttc ttcgccctgc
     2401 ctatgctgct cggcgccatc gtggtgctcc agatgttcaa gacctccaag tccatctgga
     2461 agatcgtgct ggtgctgacg ctcttcggct gggtcggcgt atgccgtatc gcccgaagcg
     2521 ccgtgctgga atcgaagaac ctcgaattca acaccgcctc cacggccctc ggctccacgc
     2581 ctgccaggaa tctgttccgc catatcctgc cgaactcctt ggccccgatc atcgtcatcg
     2641 ccaccacttc gctggcctcc tacatcgtgg ccgaagcaac cctaagcttc cttggcgtcg
     2701 gtctgccgac caccacggtg agctggggtg gcgatatctc cagcgcacag accaacctgc
     2761 agaccgatcc gatggtgctg ttctacccgt ccgccgcact ggccatcacc gtgcttgcgt
     2821 tcatcatgat gggcgacgcc gtcaaggacg ccctcgaccc gaagagccgt acggcctgag
     2881 tgaggggagt ggagaatcat tatgactgac aacaccaacg ccaagatgct ggctatgcag
     2941 aaagagcatg gtccgctgct cgaggtcaag gatctggcca tcgacttcac cactga
//
LOCUS       DAWBMW010000192         2962 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_6751_length_2962_cov_2.406605, whole genome shotgun sequence.
ACCESSION   DAWBMW010000192 DAWBMW010000000
VERSION     DAWBMW010000192.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2962)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6751_length_2962_cov_2.406605"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    583..592
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          564 a          802 c          914 g          672 t
ORIGIN      
        1 gtcatgcaca tcaagaccta ggtggtgggc cacgccgcag gcgagccaac ggcggtgctg
       61 ctggccttgc ggggacagtg actcctcgac gctcaccggc agcagacccc aatcgtgcag
      121 acgctcggcg atcacacgca tgcaggcgtg gtggatgtcg gaataggtgg cacccggctt
      181 ggcggcttcg aaaccggcct gctgcgagtc cagtacggcc tggtacagac gcttctggaa
      241 gtcggtgaac ttgccgttgg tcgggaaggt gcgggtgatg tcggccgtgt acagggaatc
      301 gacctcaacg ccggcatcca ccagcagcat gtcgccggtc ttcaccacac cggtattgcg
      361 catccagtgc agaatcgggg cgtgggggcc ggaggccacg atggagtcgt aaccgatggc
      421 gttgcccagc tcacgggaca cggcgttgaa cgcgccttcg aggatacgct cggaacgcgg
      481 cttgtcgagc gcggccggca gtgcggagag cagacggtcg aagccgtgct tggtggcgtc
      541 cacggccttg cgcatctcgt tgatttcgta gccgtctttg acnnnnnnnn nngcttgtcg
      601 tcggatgcgg agttcttgtc cggatcggcg aaaccgttgg cctcgcggat ggactcgacc
      661 atcgaggtga tttcgggatc ggtctcgctg accacgcgca cacgcacggc gccggcctcg
      721 gcacccacgt ccttggacag ggcgtcggcc agctgggcga tgtcgtgggt ctcgatgccg
      781 gtcatcgcct gcatctcctt gaggccagca cgcgggccaa cccagtattc gccgtagtgg
      841 gcggacatga agaagtcctc ggtggagttg tcggcgcgag gtgccacgaa cagttccgga
      901 gtgtgcgtct tgccggcggc ggcttcgggg gagtcggggt cgaccgggtt caggaccagc
      961 acggcgccgg cctcgtaatc ggaacccaga ccggtgtagt aggcgaacgt ggtgtccggg
     1021 cggaacatgt aatcgcaatc attgttgcgc accttcggct ggccggccgg aatgaccaga
     1081 cgctcgcccg ggaaggcctt gcccagcgcc tcaaggcggg ctggcgtgaa cttggaggat
     1141 tcgagaggct tgatctccgg atcatcgtcg gcccagccgg acttcataaa ctccttgaat
     1201 gcagccgact gcgggcgcag cgagcggttg ttcacacggt cgctcatcga ctggttcgcg
     1261 cccggcgccg tagcgcgctc ctcggcctca tattctttgt ccttggcggt gatcacttca
     1321 cccattgcca ctctcctgta tgtagttgtt gtcgaatcat cgattcccac ctcatattag
     1381 tcggctttat gcgatgtgcc acgattgacg gctgacggta aaaccaagta cggaatttgg
     1441 acggtcttcc ggacatgccc atatggcgag tgcggcgggc tggaatgcgg gcccgtttat
     1501 tgttctcggt agggaaggag aggggcatgg gcttgggctg gagcttgccg attgatattg
     1561 acatattctg tgggctgaat ccatgtgaac ccatgcgatg aaggtttatc gcgtcgcggg
     1621 actcactgct atgcggaggt ctccacctga ggcaccccgg ctttgatcag ttttacccgc
     1681 agcggagtac gttcgatgac gtcggcgtat gtgaaatgga cgatttcggt ggcaccggct
     1741 cgtttcagtc cctcgtcgcg tgcgcgttcc ttggccacca cctcctgaat gctgcggtta
     1801 tcggtcatgg ctgggtccac gtatttttgt gtgccgtcgt attcggccac tatcacgcgc
     1861 ccgtcatcga gcttccatac gaaatcaacg cggtactcgg tattcgtctg tgggtcggtc
     1921 accgttacct gaatacgcgg cgtgaggaag cgatcttcga tgatggtgcc tcgcgcgagg
     1981 gattctccac cattctcgct cctttcgttt gcatagcgca gcaggcggaa tactcgggca
     2041 taatcgattc tccattgaac gcagtcggcg gctatctctt ccttggttat tcccttggca
     2101 agggccgagt caaagatcgg taacgcaaac cgaaaatcaa ggtctatagc ggcctcgacc
     2161 aaggttctag ccggtgatat cagggatagg ccggaggcct cgtaatgtgt cttcggattg
     2221 ttcatatcgg gcatatagac atgtcgtaag cgctgatggc attgacgaga tccatgatcg
     2281 gaagtcacga tggttatgca gtcatcatgc aaatgccatt gatgttcgaa tccataggca
     2341 cttactgcca ccaagccgcc aaaaacccac tgaggatgct cttgcgcaag agcgcgggct
     2401 atatgcaacg ttcgctccgg cggcgtcaaa ccatcccagt atgcagtatt tgcataaaga
     2461 ctgggaatgc cgtcatacac tttcaacaac tcaccactct gggcacggcg gcgtagagcg
     2521 gtctgttccg cttcatttct tgaatagacg catctcagct cattctgcgc ggctgatatg
     2581 agcgaggcca ctgctttgtg tgtcttcatg acgtcgagcg tagccggaac gaagcagcgt
     2641 aagtatgaat ttcagccatt gtggttctag cctcggagct tggctgtctt ggagcgagat
     2701 actcccgata atttcaaggt attaggccgc taattcggaa tagtggataa cacacaaaac
     2761 ttatccacca tcagtaatgt cattcgaaca acgtgctttg gggattcgaa caagatatca
     2821 tcggcgaagg cggatgtagc tggtagaggg cggcgaattg acggttaacg gtgcttatgc
     2881 tttgtacgcc gtcggtgctg gttcagatat tggctggctt ctgtctgagt gtggaatcta
     2941 gcgtcggcgt gagtccaatg gt
//
LOCUS       DAWBMW010000193         2946 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_6801_length_2946_cov_2.428226, whole genome shotgun sequence.
ACCESSION   DAWBMW010000193 DAWBMW010000000
VERSION     DAWBMW010000193.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2946)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6801_length_2946_cov_2.428226"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1781..1790
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          675 a          845 c          889 g          527 t
ORIGIN      
        1 accattggac gaatggctgt cttgtcttga tgccgggaac accgacctag gccgagagac
       61 gatggccgcg tttgcggtgg gcatgcgtga gaccttatcg attcgtgatg cgctgattct
      121 ctcgttggtt attgatgaaa gccgttgccc caagtcatgg cttatggaat tcgcggcgcg
      181 accgcatctg gcgcgtacga gaacacgaat gggcgagctg ctgacttgcg cattcgagga
      241 tgaaggcatg atgccggata aggagcgttg ccatgccggc atcggtatgt tgctcgatat
      301 cgccgaagcc tcaccggtgc cgtactgcat acagccattg gccgtaatcg cctacacctt
      361 atggtggctg ggcgatccgc gagcaatggt gttcgccttg cgatgcctgc tgctcgatga
      421 ggattgttcg ctggcggcga tgatattctc agccgccgat cgaggcgtgg caccggcatg
      481 gtgctcgggc tgagtgaaca gaaacggcgg atttccgagg ttttacgcgc gatgcgcgga
      541 aggaaagcgt gtggtgatcg gcaacacgtc gggcaacgat gtgggaataa tcgtccgtgg
      601 agggtggttt actcacatag ttgacgattt gcccttgcca gaagtgggcc catgtggtat
      661 atgcacttca cggcgagaga tcaggaggag cagttttggc cacgaaggaa acgacggcaa
      721 ccaagcagac agccgagtcc agcgaggaga ccgagaccaa gaagaaggcc agctctacga
      781 ccaccgcacg caagacttcc gccaagaagt ccaagtccgc agagccctcg aaaaagacca
      841 cgcgcaagtc cgctgcgaag aaaaccgcga agaaggccaa ggacgatgag ctcgtcaaag
      901 acgaggacac tcaggtcgat gacgagcttg atgacgatgc cgaggtcaac gacgacgatt
      961 tggacgttga cgacctcgac gaggatcttg acgacgttga cgaagacgat gccgacgacg
     1021 aagacctcga tgaggacgat gccgacgacg atgatgtgga agacgacgag gacgaggaag
     1081 aagccaagtc caaggcaccc gagcagccca aggaaaaggg tgcctacgtg gtctccgaca
     1141 cggatgatga ggaagagaac atcatcccct cgggcaatcc gaagcgtcgt gtgattgcgg
     1201 ccggcgcaac cgccgacccg gtcaaggact acctgaagca gatcggccgt gtgagcctgc
     1261 tgaatgccga gcaggaagtc gacctgtccg agcgcatcga ggccggcctg tacgcccagc
     1321 acctgctcga caccgaaagc gaaggcatgg agttcaagcg caagcgtgaa ctcaagtggg
     1381 ccgccgccga cggcaagaag gctaaggacc atctgctgga agccaaccta cgacttgtgg
     1441 tctcgctcgc caagcgctac acgggtcgcg gcatgctctt ccttgatttg atccaggaag
     1501 gcaaccttgg tctgattcgt gccgtcgaga agttcgactg gaagaagggc ttcaagttct
     1561 ccacctacgc cacgtggtgg attcgtcagg ccatcacccg tgccatggcc gatcaggccc
     1621 gcaccattcg cgtgcctgtg cacatggtcg aagtgattaa caagctgtcc cgtgtacagc
     1681 gccagatgct gcaggatctg gggcgtgaac ctacgccgga cgagctggcc cgcgagctgg
     1741 atatgccggt tgagaaggtg caggaagtgc agaagtacgg nnnnnnnnnn agccgatttc
     1801 cctgcacacc ccgcttggcg aggatggcga ttccgagttc ggtgacctca ttgaggatac
     1861 cgacgccatc gctccgtccg acgccgtggc cttctccctg ctgcaggaac agttcaagca
     1921 ggtgctggaa accctgtctc cgcgtgaggc cggcgtcatc aagatgcgct atggcctgga
     1981 agacggccag ccgaagacgc tggacgacat cggccgtgtg tatggcgtga cccgtgaacg
     2041 catccgtcag atcgagtcca agaccatgtc caagctgcgc cacccatccc gttcgcagac
     2101 cctgcgcgac ttcctggatc aatgatcatc tggctcccct ctgtgagggg agccaagcat
     2161 ttgaagagaa aaataggact gtatgcccaa agaagaatac ggtgccaagg acctagctgt
     2221 tcttgagggc ttggatgcgg tacgcaagcg tccgggcatg tacatcggca ccaccgactc
     2281 gcaggggctt atgcactgcc tgtgggagat catcgacaac tccgtggacg aggccttggc
     2341 cggcttctgc aacgacattg tggtcaccct gcacaccgac ggttccgtgg aagtcgcgga
     2401 caatggccga ggcatcccgg tggacaagga accgaaaacc ggcctgtccg gcgtggaggt
     2461 cgtactgacc aaactgcacg ccggtgccaa gttcggcaac tcgtcttata acgccgtcgg
     2521 tggcctgcac ggcgtgggct cctccgtggt caatgccctg agctcccgac tggacgtcga
     2581 agtggatcgt gacggcaaga cgcaccacat gaccttccac caaggtcacc ctggcgtata
     2641 cacggatgcc gacccggcca atccgtctcc cgattccccg ttcaagcgca ctcgcaagaa
     2701 ccgacccacc gagctggaaa tcatcggcaa ggtatctccc aaaaccactg gtacccgtat
     2761 tcgctactgg tatgatccgg agatcttcaa caaaaccgcc gagttctcat acgaacagct
     2821 catcgaccgc gtgcgtcaga ccagcttcct ggtgcccggc ctcaaaatca ccatcatcga
     2881 cgaaaacgta ccggaaacgg cggcgactga caccgtcgag gccactgatg acgcaacagt
     2941 cgaacc
//
LOCUS       DAWBMW010000194         2927 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_6858_length_2927_cov_2.300487, whole genome shotgun sequence.
ACCESSION   DAWBMW010000194 DAWBMW010000000
VERSION     DAWBMW010000194.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6858_length_2927_cov_2.300487"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    719..728
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2686..2695
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          555 a          811 c          957 g          584 t
ORIGIN      
        1 gtcggcggtg ttggtctggc cacatgcggc gaaaccgaaa gtcatggcaa gggcgcccac
       61 gagggcagcg gccttggata caaggctttt cttcataatg atggttccct aattcgttgt
      121 ctctctatcg ttctctggga ttgcttcgaa cggaagtcgg cagaaaacga tatggagggc
      181 cttgatttcc gttcgaccag cgcccccgac attccctatg aaagctcggg aaagccggtt
      241 tgccacacgc tggtaaagct gttccattat agagacaggg ttggagacct catgagaagc
      301 cgcttcgttc agtcggtcct ggccgccggc tcgtgccttt gacggtaaaa aaagaactac
      361 tggtccgcgt cggggtcttc gtcgtcgccg ggcgggccga gcaggatgcg ctcgctggcg
      421 tttaccgcca gcatcagtac gcatgtcatc aggataatga cgatggtggc ggagaagatc
      481 agcggcgtgc ggaatgaatt gtaggcagcc tgcagccaga tgccaaggcc cttgcgtgcg
      541 ccaaggtatt cagcggtgga tgccgtggcg aagatgtatg cggcagatac tcggataccg
      601 ccatagatct gacgtgcggc aacgcgcagc ttcacgtgcc agagcgtcca gaccggggtc
      661 gcgccgcagg tcaatgcgac gtcggaatag aactggggca ctgcttctag tccgcgcann
      721 nnnnnnnnag tctgcacggc gatggggaag aggccgaata tcgccacgat tacgattttg
      781 ccggaaatct caaaaccgaa ccaaatcagg aacaacgggg cgatggaaat cagcggcatg
      841 gtttgtgcgg cggcaagcag cggatagaac gccgcgttga acgtgtgcga gcagtacagt
      901 gcaatgccga tcataatgcc gaagaacacg gcaaacagga atccaaccac gccctcatag
      961 ccggtgattg ccgcggccgg gccgagcgtg ttccatgttt cgatggtggc ggcgaaaatc
     1021 tcgcttggcg cggagatcat cgtgggggag acatgtccta gatccaccca cgtttcccag
     1081 actgccagca atacgatgac ggcgatgacg gtgggcatgc ggcgcttgag gcgcttggcg
     1141 atggacatgg gatgtcacct tgctttccgc gccagtccgg tttgctggtg gcgcttgcgg
     1201 ccggggcgtg gaacagtaag gtgaggagcc tgcgcgcctg ccggtcgtgt gatggattgt
     1261 gttatatgcc gtccatcagt ataggcgaag gcgaacgaca gggcgcacaa aaaaacctcc
     1321 ctcgtcagag ggaggtgaaa gagctactcc agtccgatgg acttcatgtg gtggagcttc
     1381 agggaatcca ccttgagact accggactcg gcgacgagct tgatggcgcg cgggccttcg
     1441 gaggcgtagg agtaggagct cagcacttgg tggccaccgt tgacgtagac ctccacggag
     1501 ccgcgatcca cgaacacgcg caggtccagc ttgccggagg ccagttcggc gtcggtcagt
     1561 ggggcggcgc ggtagccgcg atcgccgttg gccatggcct gacgatccac caccacgcgg
     1621 ccgatctggc cgtcataagc cacgtaggtg taggcaccgt cctcagtggc gtgaatcttc
     1681 agacccgcac gctcggcagt ggaggcagcc aagtcgatgg tcatttcgat ttccacggcc
     1741 tcggcgtcgt cggcgatgat ctgctcgcca tccatgtcga gtgtgaccga accatggtcg
     1801 agggtatcct cgcgcaggcc ttccatttcg gcgaccggcg cggtgaccac gtcgccgtca
     1861 tcgccaaggg tgatctcgcg cggcagggtg agctggccac accagccgtc atcctccatg
     1921 ggaatcggct gcacgaacgg gctcatccag ccatagacga tctgacggcc gtccacattg
     1981 aaggactgcg gggcgtagta gttgtggccg caatcccaca ggcggaactc ggtttccggc
     2041 ttgaattcgc cgcccggctc ccacgtgccg atcatgtagc cggcgttgct gacgttgcgg
     2101 ttcatgaagc cgctgggctt ggaccccatg gcggagaagc caatgaccca cttctcgttg
     2161 ccgtccttgt ctttgatggg gaagaagtcg gggcattcga gcatgaacac atccggatcc
     2221 gggtgctgga agagcacgcg ctcgtattcc cagcggacca tgtccttgga ggagaacagc
     2281 cacatctggc cgcgcttgtc cgcagaggag acaccgaagg tcatgtacca ggtatcgccg
     2341 gtcttccaga ccttcgggtc gcggtagtgg tggtcgacct tgtcggtcgg gcagtcgatg
     2401 atcatgccct gcttggtggc ggaggtgagc tcgtcattgt ccggcagcgc ggtcatctgc
     2461 acctgccagt cgccgccggt gttgtcgtgg ccgttggccc aacggtggcc ggtgtagtag
     2521 aagcgaaggt ctccgttgtc gtcgatgacg gcggaaccgg agaacacgcc gtccttctcc
     2581 tgctctaagg acggcgcgaa catgatgggc tcgcgcttcc agttgagcat gtcggtggag
     2641 gagacgtggc cccagtgcat cggaccccac tgggtgccgt aggggnnnnn nnnnnttgga
     2701 ggcgatgtga tacttcgggt accagcggtt gttgcgcttg gcggccatct cagccacgcc
     2761 ggcttcggct tttgccagtt cagcagcgtg gtcgcggatg ggggtgagaa caggggtttc
     2821 gggagtgaag tcagtcatgg ctgtcctttc aggccttggc cgcttccatc tgacgcagct
     2881 gcttgtcggt gtagaacggg tcgccgccga cttcctgatc atcatgc
//
LOCUS       DAWBMW010000195         2890 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_6939_length_2890_cov_2.715344, whole genome shotgun sequence.
ACCESSION   DAWBMW010000195 DAWBMW010000000
VERSION     DAWBMW010000195.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6939_length_2890_cov_2.715344"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          538 a          840 c          933 g          579 t
ORIGIN      
        1 gataggatca taccgccgaa cttacccatt tcatcagtgg ccatctggcc cagagagcga
       61 ccgcggcgct tggcggagat ccacagcacc agcatgtcct gcacggcgcc ggcgaaaatc
      121 acgccgagga tgatccacag agtggagggc aggtagccca tctgcgcggc caggatcggg
      181 ccgacgagcg gtccggcgcc ggagatgccg gcaaagtgct ggccgaagag cacgcggcgg
      241 tcggtgcgtt cgaagttcgc gccgtcatgc acgcgctcgg ccggggtggc gttcgcgtcg
      301 tcggtgcgca tgatcttgat ctggatgtaa taggcataga agcggtaggc gatggcgtag
      361 gagctcagcg ccacgaccag gaaccagatg gcggagatcc gctcaccgcg cgagacggcc
      421 agaatcgccc atccgcaggc acatacgatg gcgatggcgg cccacagcag ggccttcttc
      481 catgtccata ccattttggg acgcacgcct acgggcagtc ccttcgaatt gcgaatcaca
      541 cgttcggctt cgtccggcgt gtaatcgtcc ttgaattcca acttgccagg tgccggggcc
      601 tgggctgcgg cggtggtcat aataaccctc tgttcctctt caataacctt tgcgggaaag
      661 gttctcttca ttgcgaccct tttggggtat ctctcgtctg cttatggcac tggttggaag
      721 cgtagccacg gcatgtcgcg cctcaggaaa atgtcccaat atgtgagctt ggccacatgt
      781 tcacggactg cgtttcatgc cgattacccg ggattcgcac cgcgatgtgc gaaaatggac
      841 acggtgtcaa tcaggaggtg cacagtggtt gtaagcatgg attcgagccg cacaatgccc
      901 gattgggtga agtacggggt gttctggcac gtgtatccgc tgggcttctg cggtgccgat
      961 atcaggcccg ccggcccgcg ccagttccat ggccgcggcc ttgacgcgat aattccctgg
     1021 ctggagtacg tgcgcgatct aggcgcgtcc ggcctgctgc tgggtccggt gttcgagtcg
     1081 gcgacgcatg gatacgacac gctcgaccat atgcatatcg atgtgcgatt gggcggggat
     1141 gccgcgttcg atcagcttgt cgcggcctgc cgggacatgg gattgcaggt gatgctggat
     1201 ggcgtgttca atcacgtgag ccgcaatcat ccggccgttc aggcggcgct tgacgagacc
     1261 gccggacgct tgaatccggc cgacaacccg tggcatggtc tggtccgcgc gcatgtcggt
     1321 gataatggcg aacccgcttt ggatgtgttc gaggggcacg gggatctggt ggcgctggac
     1381 cattcggctg acgagaccgt tgactatgtg gtccatgtga tgagccattg gctggatcgc
     1441 ggagccgccg gctggcgcct cgacgccgcg tatgccgtgc cgagcccgtt ctgggcgcgc
     1501 gtgttgccgc aggtcaaagc cgcacacccg tattcgtgga ttttcggcga ggtgatccat
     1561 ggcgattacc cgcgcatcat cgaggaatcc acgatggatt cgatcacgca atacgagcta
     1621 tggaagtcga tccagcatgc gctggagacg gagaacttct tcgagctgga ttggaacttg
     1681 aagcggcaca acgcgttcct ggattccttc gtgccgcaga cgttcatcgg caaccatgac
     1741 gtgacgcgta tcgcatcgca gattggcccg gccaaggcgg cgttggcatt ggcggtgctc
     1801 atgacggtcg gcggcgtgcc aagcatctat tacggcgacg aacagggcta tgtgggcgtc
     1861 aagcaggagc gtttcggcgg cgatgacgac gtacgtccga agttccccgg ctcccctgcc
     1921 gaactgtcca cgctgggaga gccgacatac cggctgcatc aggcgctgat cgcgctgcgc
     1981 cgtcgcaacc catggctgct ggatgcgcgt accgaggccg tgaagctgga aaacaagcac
     2041 ttcgtctatc gttcgacgag cgccgacgcc cagcattccc tgaccgtgga cctcaacatc
     2101 gagcaatccc ccactttcac catccgcaac gccgatggca gtacagcgta tcaatggtta
     2161 gctccctcat cagagggagc taactcattc acctctgaat gcctcggatc agcaggtcga
     2221 gcaggtaacc gtacgaccgg tcgacccgtt cgcggccgcg tgtgcggggt tgtgcggaac
     2281 gcagttcgaa ttggatgaac ccgtgtatgg acacccagat ggaggatgcg attagatggg
     2341 ggtcatcgcc gcggaataca gatgctgcct ggccgtatcg aatgatctgc gtgatggcgt
     2401 ccaggcagtg ctgggccgag tccgacgcct gccccatcca gaccagcgcg taaatgccgg
     2461 gggcgtccag cgcgtgttca cggtagatta tgcccgccct gcgcaggcgc agcacggggt
     2521 cggattcgtc aatggcgatg agctgttcgc gcagatcggt gaagtatgtg tcgatgagcg
     2581 catcgcacac cgcctgcttg ccgccgatgt gctggtagat gctggtgggc gacacatcgg
     2641 cctgtgcgga cagcgcacgg atggtcagac catccacacc gtcccgttcc acgatccgcc
     2701 ttgccgcgtc aaggattctc ccgtggattt caagggtggg cgtgcgtttg gatcggggct
     2761 gcgattgcgt catatccgtt attctagggt gcgtacaggc cattaccgcc gtttcggcat
     2821 ggccacgatc acaacgactg cggcgataag tgcaatcgtc atggccgcta ggcaggcgaa
     2881 caggcatccg
//
LOCUS       DAWBMW010000196         2815 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_7147_length_2815_cov_2.712681, whole genome shotgun sequence.
ACCESSION   DAWBMW010000196 DAWBMW010000000
VERSION     DAWBMW010000196.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7147_length_2815_cov_2.712681"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2161..2170
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          602 a          842 c          790 g          571 t
ORIGIN      
        1 gaacccgagc ttttccatca tcaaaccgtt gctctcccgt tcggtggtcg tcaagcttga
       61 gtcattggag cctgatcagc tcaccgaact ggtgcagcgc gcgctgactg atgaccgcgg
      121 tctcagggac gaggtcaaag ccacggacga agccatcgcc gatatcgtgc gcatggccgg
      181 tggcgacgca cgcaagagtc tcacgattct ggaggccgcg gctggcgcgg tcaccggcga
      241 cgaagcccgc aaaaagggcg cacgccgacc catcatcacc tccgaaatcg tggctacggt
      301 aatggacacc gccaccgtgc gctacgacaa agacggcgat gaccattacg acgtgatttc
      361 cgcattcatc aaatccatgc ggggctccga tccggatgcc gccatccatt atctggcgcg
      421 catgctgaaa gcgggtgaag atccgcgctt catcgcccgt cgcatcatga ttgccgccag
      481 cgaggaagtc ggcatggccg ccccgcagat tttgcaggtc accgtggccg cggcgcaggc
      541 cgtggcgctg gtcggcatgc ccgaggcacg tattattttg gccgaggcca cgattgccgt
      601 ggccaccgcg cccaaatcaa atgccagcta taacgccatt aaccaggcgc tcgcggacgt
      661 ggataccggc aagatcggcg ctgttccgct gtatttgagg aacgccccca ccaagcttat
      721 gaaagagtgg ggcaaccacg agggctacaa atatgcgcac gattggcccg gtgccgtggc
      781 gccacaggaa tatatgcccg aagagctgcg tggcaccgag tactaccatc ccaacgatcg
      841 cggctatgaa cacgaagtca gccagcgtct tgccaagatt cgcccgatac tgcatggtgg
      901 ggaacccgaa cagaaatgaa cataggggag taaagtgtaa cgcaacaata acagtggaaa
      961 aaggggaggc aaacaccatg gcgaccatct tcatcgtgga cgatgatcag gccatcggcg
     1021 aaatgcttag tcttgttttg gaaaacgagg gattccagac tgtgacctgc ctggacgggc
     1081 tgagggctgt ggaaatgttc cccatcgtca agcccgattt gattctgctt gacgttatgc
     1141 tccccgggct ggacggcacg gaggtggccc gccgcatcag agctacctct aatgtgccga
     1201 ttatcatgct caccgccaaa tcagacacac ttgacgtcgt ggccggcttg gaagccggtg
     1261 ccgacgacta cgtgcccaag cccttcaaag tagccgaact gctggcgcgt atccgtgcac
     1321 gcttccgcat cgccaagccg gccgccgaag acggtgccac cggtggcgca agcggtggca
     1381 atgcaaacgt caaccatctg gaacgcggac ccatcgtcat cgaccgtttg gaacacaccg
     1441 ccaccaagga tggcaaggac ctgaatctca ctccgatgga attcgagctg ctgttcatgc
     1501 ttgcggccgc cgccggcgag gccatcagtc gttccagcct gctcaagaat gtctggggat
     1561 acgagaattc cggcgatacg cgcctggtca atgtgcatgt tcaacgcctt agggccaaag
     1621 tggaggacga ccccgagaac ccgcaaatcg tgcagaccgt acgcggtatc ggctacaagt
     1681 tcgtcactcc tgaacaatga acctgcgccc tcgattcagc ctcaaacgct tgctgcgcca
     1741 cggacgcgct gaagttcgtc gctcactgca ggcccgcacc gtggccctca ccgtgattct
     1801 tacgctggcc gtggccatcg tcttctctgg cgtatcgatg gtatcagtgc gcgcctcgct
     1861 gcttacccaa atcacttcgc agtcgcgtgc cgactattca aacatggtgc agcaggccca
     1921 aaccagtctg gacgcggccg atgtctccac cgcagtgcag ctccaacagc tggtcaatga
     1981 cttggcgtcc tcgttgcaat ccgaggggtc ctccaacctg ataggtgtgt atttgtggag
     2041 tcgtgatacc aactcgcgtg ccatcatccc cgtatccacc gaacccagct atcaaagtct
     2101 gatttctgac gacatccgtt catccgtggc ttccgacttg gatgacagcg tgttctatca
     2161 nnnnnnnnnn gtcgaaattc ccggtgattc cggcatgccg ggcagcggga cgcccgcagc
     2221 ggtgctcggc accgtattgg actttggcgt ggccggcaac ctcgaattct tcgccattta
     2281 ttcgtacacg ttccagcagc agtccttgac gcagattcag ctcagtctgg tggtcatctg
     2341 tgcgttgctg tccatcgtgg tgggcgtagt aatctggctg gtgattcgtg gcatcgtgcg
     2401 cccgattgaa cgagtggcag cggcctcgga aacactggcc tccggcaatc tcgatatgcg
     2461 cgttaccgtg gatcgtaagg atgagcttgg tgtgctgcag caatccttca atacgatggc
     2521 tgatgcgctc aaccagaaga tcgatgagct ggaggaagca agcgttttcc agaaacgatt
     2581 tgtgtccgac gtcagtcatg aattgcgtac cccggtgacc accatgcgta tggcttccga
     2641 cctgctcgaa atgaaaaaag acggcttcga cccctccacc aagcgtacgg tcgaactgtt
     2701 ggccggccag atcagtcgat tccaagacat gctcgccgat ctgttggaaa tctcccgcta
     2761 cgatgccgtc aacttggtcc actgccgttt caattggttc gcataaatcg gtttc
//
LOCUS       DAWBMW010000197         2800 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_7192_length_2800_cov_2.319126, whole genome shotgun sequence.
ACCESSION   DAWBMW010000197 DAWBMW010000000
VERSION     DAWBMW010000197.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7192_length_2800_cov_2.319126"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    766..775
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          533 a          780 c          838 g          639 t
ORIGIN      
        1 taaccacgaa gaacgcgata atcgcgatgg cgatcagcgt ccagctgccc acgcggaacc
       61 actgacgcag cgaatgcggt gccttgaaca tcttcggatt cgtgtagttc gggttagtgg
      121 tcagcagggt ttcggtgtcc gcgatcgtac cgccgctggg cttccattcg gtgccgtcgg
      181 tgccgtcctg cagacgctgg gacttctgct gcagctggta ttcgtcaatc attcccgagc
      241 gaccgcggcc acgggtctgg tcatactgcg acggcactcc cctgcggcct gctttggctt
      301 gggcacggcg ttctgcacga ttcggcattt cactactcct gtttcgacgg tgacactgtt
      361 ggctatatta ctcgcttgtc ggcggcaatt tgccaccagt ccatcggaac ccactgtgtg
      421 tcattgtatt ttcagtcggt gatgcgatag gtttgcgtat ggaagtcgca agtgaccgca
      481 gtgccgtccg cgaataccga acgctgcacc agcggatcat catccacgaa ctcgtgacga
      541 accagttcct gcatgccgac ccgctcatgg aatgcagcga ccgtatggca gcgttcgatg
      601 tcgttttccg tgcgagcgcg ctgttcgtcg tccatgtcgc cgtcaacgcc gatgtatgcg
      661 gcatcgcgaa tcaggtatgg agctccgccg ttgagcaagg cgtacagcat gtaatcgtcg
      721 ccgttcacca cacgctccat catccatggt tcgataacgc aatcannnnn nnnnnatcat
      781 ggtagacaag gttatacagc ggcaccggca caccttggcg cggctcgttg ggtgagcgca
      841 tctggaagtc gtatggtgcg taatggcaga atatcaggct tggcactgcc cagtccgata
      901 cctcttcgga cgaggagagg atgccgtgcg agagcagata ttcgaagcat tcggcgcggc
      961 ggtcaaagca ttcgcggcgg gtcatgcggt gttcggggtt cgagcattcg tcgccttcgt
     1021 tgcaggtgaa aacatccaag tatgcacagt cgagcttgat gccatgcgcg gcaatctggg
     1081 tgaaattgcg gcgtacgtag tccggtgcga gctcggcgca caggtaggtc tggtggccgc
     1141 ccgcccagcg tgcgtgttcg ggcatcgtgc cgtctgccag ccgaatcgca ttgttggcat
     1201 caaaggtttg cgcggtgaag tagtaatcgc ggtactggtc gtgcgtgccg aagatatcac
     1261 cttgctcatg acaggcatcg accaacgatt tcatgccttc ccagccgccc gcttcctggc
     1321 aggccggcag atagtcgggg tgtgcgttgt cgtatccggg ctgcgcccaa ccatccaagt
     1381 gcatgtacag tcggcctgca cccatgccgt gcagtgtgcg catctgcttc tcgcgctgcg
     1441 caaaggtgac caatgaatcg ttcttttcgg gatggtcctt gtcatagtag aacgagtcgg
     1501 gctgcacttt ggttttgatg ccgacgtgca cccatgaacg accaatcagg tcacgcacgg
     1561 agggattgcg cgccgccttt tcggcgagcg tacgcaagcg acctcgttcg ttgacgtacg
     1621 aacgataggc cttgcacacc gccgtgtgat cggcgtggtc gaggaactgg tagcgtacca
     1681 cgcggcgata attcattgtg ccgaggcttg gctcaaacca agtattgata tgggtgtacg
     1741 gaccattgct gggatggtcg atgccgtaac cggcattcca tggggtttcg cagatggcga
     1801 tatacccgtg tccgcctgca cgaagctgcg cgaaccacgg catgtaaccg ccagccgttt
     1861 cgaagcggcc gtcgaaggcg atatctttgg tgctcacggc ggtaggccat gtgttgggga
     1921 tcatcacacc ttgctcatgg gtgatgagtg tcgtgtcatg gtcgtcggcg caatcgaagt
     1981 ccatggctgc gggccacgtc acattagtga tgttcaggcc ttgctcattg agcggaatcc
     2041 attcgaagag cacatcgcct gaggcacgct ccacccatac ataggtttca aaggagtatg
     2101 cgctgtgccc aaagccggtg aagatgctgc gaatgccggt tcccgtgccg gtttcacgct
     2161 gctcatgggt gatggaagtg gcatctgcga acgcgaaggt gccttgcgcg cattgcaggg
     2221 tcggcttgaa atcagcgcag gtattccatt cggcaccgtc gcgcgtaaaa gcgaactgca
     2281 ttgttcgttc atcgaatcgc acggtagtgc cggatacggt gaattccata gttaatgtgc
     2341 tcctgtatgt tctcgattgc tcccgcttac gggagctcgc tcatataaca agattagggg
     2401 tggtctgctg tttgagacca cccccagtcg gctttgccga cagccccccc gccagcgggg
     2461 gccaacagag gtcagattgg ctcagcccat ggtaatcggt cggcaacccg acctatagca
     2521 aggccaactc tttccagcaa agtcgcgctt actgcttctt ggtcagtgcg gtgaacactg
     2581 ggccgccacc gtaatcgaat tcaacgccgg aaactgcgga ggtgctggct gcggtggcat
     2641 tctggttcca caacgggata gccggaaggt cctgcagcag aatctcctca ccttgctgat
     2701 aatacttgtc ggcttcctcg gtggacggag cggaaagagc cttgtccatc atggcatcga
     2761 acgcggggtt cttgtagttg ccgctgttgc cgcccttgcc
//
LOCUS       DAWBMW010000198         2764 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_7304_length_2764_cov_2.433739, whole genome shotgun sequence.
ACCESSION   DAWBMW010000198 DAWBMW010000000
VERSION     DAWBMW010000198.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7304_length_2764_cov_2.433739"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          511 a          845 c          887 g          521 t
ORIGIN      
        1 gaccacgatg gcctgcttgc gggccgtgac atgctcgata tgcgtggcgg cgcgcgtggt
       61 gagcgtacca cggccagtgg cataggcctc acggatgccg tcgcggccga tgatcgtgcc
      121 gccggtgggc cagtcggggc cgggcacgta acgcatcagc tgttccaggg tggcgtccga
      181 atgcgccatg aggaacttgg cggcgtttac cacctcgccc agattgtggg tggccaggtt
      241 cgtggccatg cccacggcga tgcccgagcc gccgttgacc agcagattcg ggatggcggc
      301 cggcagcacg gtcggttcct tgagcttgtt gtcatagttc ggcgtgaagt cgacggtgtc
      361 ctcatcgata tccgcgttca tgccgagagc ggccgggcca agcctggcct cggtgtaacg
      421 ggaggccgca gggccgtcat ccagcgagcc gaagttgccg tggccgtcta ccagcggcag
      481 acgcatcgcg aacggctgtg ccaaacgcac catggcctcg tagatcgcgg aatcaccgtg
      541 cgggtggagc ttgcccatca cctcgcccac cacgcgggcg gacttcatat acgggcggtc
      601 gggagtcaga ttcatctcgc ccatctggta gacgatgcgg cgctgcaccg gcttcaagcc
      661 atcgcgcgca tcgggcagtg cgcgtgcgta aatcaccgaa tacgcatact cgaggaagga
      721 cttgctcatc tcctcgttca gcggcgtctc gacgatatgc tccttgaccg tgcgcggatc
      781 ataggtttgc gcggacggtt tgcggtgtga tgccatgaaa ctccctttcg aacgccccca
      841 tattagcggg agggcgcgaa acagtgcgca gagaacggtt ctcgataaca actgtgcgct
      901 ggtttcggtg cgccaatcgc acatgtttgc cgataaaacg gcgtgattcc agtgtttcag
      961 tcgatgtgcg tttgtttgat tatgtgcgtt ggagcgcaca gttgttattg agaaccgttc
     1021 tctgcgcact gagccaaggg gaggggtgct acttgaatgg gcaactatga gtgttgagac
     1081 gccggatatg gacgagttgc tgcggctggt gcccacagtg ggctatgggg atgacgcccc
     1141 ggccgatcgg cgtgggggag cactgcacct gtccgccgtg ctgcccgcct tgtccgccgc
     1201 catcggacat ccgaccccca ccaaggtcca cgccgatccc aaggcctgcc agcgcgcatt
     1261 ggggctgcct gatgccgaat cggccatcgt ggtgctcgtg gacggcctgg gctactggaa
     1321 cctcgccatg cgcctcggcc acgcgcccta tctgcgttcg ctgatgaacg aaagcgccaa
     1381 tcagcgtccc atcgctacct gcgctccaag caccactgtg gcggccatgg ccgcattcgg
     1441 taccggaacc tgcccgggac tgaccgcaat ggcgggctat acgcagctcg aacccgccag
     1501 ccataagctc atccaactca tccagttcaa agacgcgctg gcacccaaac cagccaatcc
     1561 gcacattcct gtgccgccca tggtcgatcc gctcgacctg cagcgcgagg agacggtgtt
     1621 cgagaaactt gcggctcaag aggtgcgcgt cacgagctcg gggttgccca agttctccaa
     1681 atcgccattg accgaagccg cattgcgagg caccgactat caaagcaacg tcaccccacg
     1741 cgatcgtgtg ctggccgccg cacgcgcctc ccgcaccccc ggcctgacct acctgtacat
     1801 ccgtgacgcc gacaaagtcg gccacaatta cgggtgggat tccgaacact gggtggccgc
     1861 attcgaacat atcgacgccc agctcgcgct cctgaaacgc agcgcaccca aaggcacgct
     1921 gatcgtcatc gtagccgacc acggcatggt gcaaaccgac atggaccagc ggattgacat
     1981 cgccgttgaa ccgcagctca cccgaggcgt gtcactggtc gggggggagc cgcgctcgct
     2041 gatgctgtat gccgagccgg atgaacgacc tgaggatata gcttgccggt ggcgcgattg
     2101 tctgcaagac cgagccttgg tgcggaccaa ggatgaggcc atcgcggacg gattgtttgg
     2161 accggtctgc gagcgagtcc ggccaatgct cggcgatgtg gtcgtgcagg cagcgggagc
     2221 cgtgaccctc gtcgattcgc gtacgcaagg agacaaggcc acccacctgc cgagcgtgca
     2281 tggctcccaa acagcattgg aaatggatat tccctgcctg atcgatatgg cctgattcgg
     2341 aaattcagtc tccgaacaga atctcgtccc agcttggtac ggccgaacgt cctgatttac
     2401 gcttggccgg tttggctggt gcctcatgtt cggtggcggc ggattcggcc gcccgctcct
     2461 gctcggtggc tactagctgt gcattggggc cactccgtat ggtcttgtcc gaaacattgt
     2521 cagagccctg ctctggtgcc gtggcaggcg attgctgtaa tcctgcggca gatggcgaaa
     2581 cagatccggc ggtctgcgaa gagaccggac tgggggccgt agtgccatga gcctgggtcg
     2641 tggccggtga gatggacgtg gatagatcca ctgggggcat ggaacctgtg gaagtgacag
     2701 agggccggat tgcggcaggc cgtgcggccg gaatcgaagg cgtaggagcc gcccaagcag
     2761 acac
//
LOCUS       DAWBMW010000199         2733 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_7402_length_2733_cov_2.549664, whole genome shotgun sequence.
ACCESSION   DAWBMW010000199 DAWBMW010000000
VERSION     DAWBMW010000199.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7402_length_2733_cov_2.549664"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1644..1653
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          534 a          785 c          857 g          547 t
ORIGIN      
        1 gagcggcctt gtacatctgc tgggcttcct cggtgcaggt cgggcgcatg atgtcccaat
       61 cggccagctt ggcttccagc gtcggatagt cgatggagtg cacgttggtg tgcagcttgc
      121 cggcgcggtc gagctcgccg aggatgccgg tgatgccgcc ggcgcggtgc acgtcggaaa
      181 tctcccactt gccggacggc gaggccttgc agatgcacgg cacggtgtgg gaaatacgct
      241 cgatgtcgtc cagggtgaag tccacgtcgg cggactgggc catggcgagg atgtgcagca
      301 cggtgttggt ggaaccgccc atggccacat ccatggtcat ggcgttctcg aaggcttcct
      361 tggtggcgat ggagcgcggc agcacggaat cgtcggaatc gtcgtagtac tggtgggcga
      421 tcttgacgac ctgcttggcg gcacgctcga acaggtcctt gcggtaggag tgggaggcga
      481 ggatggtgcc gttgccgggc agggccaggc cgatggcctc ggtcaggcag ttcatcgagt
      541 tggcggtgaa catgccggcg caggagccgc aggtcgggca gacggtcttc tcatagttga
      601 gcagttcctc atcggagacg ctgtcgtcgg cggtggcgta catcacgtcg ataaggtcgg
      661 tgctcttgac ggtgccgtcg gcgagcaccg tggtgccggc ctccatcggg ccaccggaga
      721 cgaacacggt cgggatgttg aggcgcaggg cggccatcag catgcccggc acgaccttgt
      781 cgcagttcgg gatgcagatg agtgcgtcgg cacagtgcgc gttgcactgg tattcgacgg
      841 tgtcggcgat gatgtcgcgg gagggcaggg agtagagcat gccggtgtga cccatggcga
      901 tgccgtcgtc cacggccatc gtgttgaact cgcgcgggat gccgccagct tccttgatag
      961 cctcggaaac gatgcggccg accttgttga ggtggacgtg gccgggcagg aactcatcga
     1021 acgagttggc gatggcgatg atgggcttct tgcccatgtc gtctccgttg acgcccgcag
     1081 cacggtagag ggctcgggcg cctgcaaaga cgcgccctga cataattttt gcagatcgca
     1141 tttcagtcat ggttctattc aagcccttgt gagggacacg ccacttgtca agagtcgaat
     1201 aatggaattt gcaaaagcgt aacgtttctc acacatattt ttggaataca ttcctccact
     1261 ggaagtctgg cggaggtccg gcagaagtcc ccccaaacgg ctacacttgc ttcggttgag
     1321 atagattttc caatgtctgg agataagaac aatggctttt ggtactgacc cgacacccga
     1381 cggtcttgcg aacccgccga tcgacgacct gatgaagcac gccgattcca agtacgcgct
     1441 ggccattttc gccgccaagc gcgcacgcca gatcaactcc tacttcacgc agctcaacga
     1501 gggcctgctg cagaacatcg gcccgctggt cgaataccag aacaatgaga agcccctgtc
     1561 catcgccttc cgcgaaatcg acgagggcct gcttgaggag accttgggcg aggacgacgc
     1621 caacgagggc aactgatcga ttcnnnnnnn nnntgtttgg acagttcgtc gccgaatgat
     1681 ggtgtgagag ttctctcaca actgattttg agcgacacgc ttgcctgagt tatcgcaatc
     1741 ggctataact caacatcggg agcgaataag acgcaaggct cgcgccaccg cgctggttgg
     1801 cacgagcctt tatttatgcc cgcttccaca gtgacaagta aaaattgaca ccgtatcatt
     1861 gtgcctcgca tcccgcgggg cgataagtcc tgaagaaagg gatgatatga cagaagaaca
     1921 tcggcttatt tccgccgaat ccgtgaccga aggccaccct gacaaggtct gcgatcaaat
     1981 ctccgacgcc attcttgatg atttgctggc ccaggattcg tccagccatg tggccgtgga
     2041 gacctccgcc gccactggcg tgttcctgat cttcggcgaa gtcacgtcca aggggtactg
     2101 cgacgtgcag tccaaggtcc gcgaaaccct gcgcaacatc ggctacacct cctccgaagt
     2161 cggccttgac gccgattcct gcggcgtggt cgtggccatc accgagcaga gcgccgaaat
     2221 caaccagggc gttgcccgtc tgactggcga tcaggagacc gccgccagcc gcgaagagcg
     2281 ttacgaagca cagggtgccg gcgatcaggg cgtgatgttt ggctacgcca ccgacgagac
     2341 cccgacgctg atgccgcttc cgatctactt ggcacatcgc ctcgccttcc gtctgaccga
     2401 ggtccgcaag tccggtgaag tgccgcacct gcgcccggac ggcaagaccc aggtaaccat
     2461 tgagtatgac gataacgata agcccgttcg tctggacacc gtgctgatct ccactcagca
     2521 cgatcctgag gttacgcagg attggcttgc tgttgagctg aagaagcacg tgattgaccc
     2581 ggtgcttgat gaggtgctgg gttccaaggt gccgcatgac aactaccgtc agctggtcaa
     2641 cccgactggt tcgttcatcc tgggcggccc ggcggccgac gccggtctga ccggccgcaa
     2701 gatcatcgtc gacacctacg gtggcgccgc cca
//
LOCUS       DAWBMW010000200         2731 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_7411_length_2731_cov_2.028027, whole genome shotgun sequence.
ACCESSION   DAWBMW010000200 DAWBMW010000000
VERSION     DAWBMW010000200.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7411_length_2731_cov_2.028027"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          555 a          842 c          795 g          539 t
ORIGIN      
        1 cgagacgcac ggaggcgtag aacttcagag ctttaccacc ggtggtggtt tcagggttgc
       61 cgaagaacac gccgatcttc tcacgcagct ggttgatgaa gatggcggtg gtgccggcct
      121 gagccaacgc gccggtcatc ttgcgcagcg cctgactcat gagtcgggcc tgcaaaccaa
      181 catggctgtc tcccatttcg ccttcgattt cggccttcgg caccaaagcg gcgaccgagt
      241 cgatgacgat gacgtcaagc gcaccggagc gaatcagcat gtcagcgatt tcgagggcct
      301 gctcgccgtt atccggctgg gagacgatga gtgagtcggt gtccacgccg agcttgcggg
      361 cgtaggccgg gtcgagcgcg tgttcggcat cgatgtaggc cgccacgccg cccttcttct
      421 gggcattggc caccacatgc aatgcgagcg tggtcttacc ggatgattcg gggccgtaga
      481 tctccacgat gcggcccttg ggtaggccgc cgatgccgag cgccatatcg agagccaacg
      541 aaccggtggg aatcacctcg acgttctgct cgggctggtc gcccagtcgc attgcggaac
      601 ctttgccaaa actcttctcg acctgcgcca gcgcggtgtc cagcgccgcc ttgcgcttgg
      661 gatcgagctc gtgcttgtcc tcggctgccg ggtccttggc cggcttggtc tcaagtgcca
      721 tgatcttctc cttgtcgaag tcgtgtgcga atcggatgcc tccaccgtat gacggacatg
      781 ccatgtgagg caatccaaac caaccattgt ggttccagct tgcccgagct tggccacctg
      841 gcctccttaa accctactgc ggtaaggatt atggcagatg gcaagcaccc tgtgcacacg
      901 tatgcggctt cttgtcactg tggataacta taaccacaca cacgaacatt tgttcgaatt
      961 tggcgtgcaa cacgccgaaa tcgccaattt cacgattcag cgactccgta atcccgcagt
     1021 gtcagacggc gggcatgggc ggcgcgttat gatcgtggcc ccaacgcttg gagtccggaa
     1081 cctccatttg gtcgcagagc acattccaga ccacgcgtgg ctcctccccc gcgtccagcg
     1141 cctcgaccac ggtcatattc gccagtttgg gcatccgttg atcacgcgac agcgaacgac
     1201 cgtaggtccg accgaacacc tcttcaagta attcccagaa ttcccgttca cgcatacacg
     1261 ccagtgtgcc accaaccgca ggacagcaaa aacccgcatc gccacaagcg atacgggttc
     1321 acagctagac atagattacg ggacgcggct tcacaccgcc aaccgatcag ccctcgaccg
     1381 agtcgagata atcagccacc atgcgcagca tgcgcgaagt ggagactccg agcgcatcgg
     1441 cgatggagct cagcagttcg gagctggctt ccttctgtcc acgttcgact tcggaaaggt
     1501 agcctagcga aacgcctgcc ttctcggaga cttcacgcaa cgtgcggcga tcgcgcgtgc
     1561 gcagctcgcg cagcacatga ccgatagcac cacgcagcga aaactcgcga ggctcctcct
     1621 cagccaccac gggctcggcg gcggcagtcg gcttgtacga cggcgaatcc tcatcccaca
     1681 tacgttcacg aacggcttga cgctgatcct tggccttctt ggcggcctgt gccttgagca
     1741 cctgctgctg ggcgaacatc accgcacggc gctgggccgg agtcagttca cgcatacggg
     1801 cgacacccgg ctgcacagcc atcggcttct tggcggccac atccatctgt tcgttcactc
     1861 gggtcatcgt ttccatcgcc atcgcccctt cccaggtcgg ttcagtgttt ccactatgta
     1921 caacacgaca acccaacggt tttattcctt aaaacttgct gtgaatagac tcaagttttg
     1981 cagaacgtgg aatacggtga ggcgacgaat ctcttcgcgc atgccatcaa gatggagttc
     2041 gacggctttg agcacttcgg catggtccgg ggtgaggctt gcggggatgc gcaggcccac
     2101 atacaccagt ccggccggct tgtcaccgtc cggaccgggg ccggccacgc cggtggtgga
     2161 aaggccgatg attcggtcgc cgtattccgg ctggctgtat aggcgagcgg taccgacggc
     2221 catctgccgg gcgacttcgg ggtgtacggc gccttctcga tccaataggg ccttgtccac
     2281 gtgcaggatc gaggccttgg cattgatgtc gtaagtgaca gcggagccca acaacacgtt
     2341 ggaggcgccg ggaatacgca cgaacgcatc gcaaatcttg aggatgccgg ccgcgagact
     2401 gtcgcacaat tcccgcattg gaatatccgt cacttccgct tcggggcgac gaacgtgttg
     2461 tacaggtaca cggcacccga gtagaggcac aagaccaatg ccacgtagat gatggcgtag
     2521 gtcaggaagt agtaagcggt catccagaac ggcgtggctt gtccgttacc cagagaccag
     2581 aacggcagca gcagcatgga cagacccacg cactggaata cggtcttgag cttgccgggc
     2641 catgcggcgg cgatgacttt gccgcccgga cgctccatga cgaagaaacg cataacggtg
     2701 ataccaattt cgcgaatcag gaacaggatg g
//
LOCUS       DAWBMW010000201         2730 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_7417_length_2730_cov_2.448224, whole genome shotgun sequence.
ACCESSION   DAWBMW010000201 DAWBMW010000000
VERSION     DAWBMW010000201.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7417_length_2730_cov_2.448224"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          603 a          892 c          748 g          487 t
ORIGIN      
        1 caacccgaag gcgctcgaag cggtgtggga ttacgcctac aacatcggca tcggctacct
       61 gggtaccaac accccgatcg accactgctt cgtgtgcggc ttccagggcg acttcgagcc
      121 gaccgaggaa ggcttcaagt gcccggagtg cggtaactcc aacccggaca agtgcaacgt
      181 caccaagcgc acctgcggct atctcggcag cccggtccag cgcccgatgg tgcacggccg
      241 tcacgaggag attgcccacc gagtcaagca catgagcggc gaaaccggtc gtgtgacgct
      301 ggatgacgga accactcgcg agtggttcga agagaccaag tagggtttat acttgctccc
      361 cgctgacggg agctgtcgag cgtaagcgag actgagggtg gtcatccttt atggtgttga
      421 ccaccctcag tccgtctgat gacggacagc tcccgccagc gggagctaat gctttccgta
      481 aggagggaat catgccggag ctgcatacga ccaagcccga gcccaagcgc catgactttg
      541 cggccggcga gaccgggcgt ggcccctccg taccctccaa cggtcttgcc aacgatccga
      601 aagccggtca atgggatggc cggcgcatgt ccaagcggat gatcgccgac tacaaaacct
      661 tcattgtcac cgacggagaa ggcgtgcgca actcccttta cgtctccggt tgcccattcc
      721 actgcgtcga ctgcttcaac gcctccatct gggatttcca agcaggccac gaatacacgc
      781 aaaaacttga agacaaaatc atcgaagacc tgaaagcccc atgggtgcaa ggcattacgt
      841 tccttggcgg cgagccattc ctcaacactc ccgtgctggt gccgctcgca caacgtatcc
      901 gccaggaatt cggtcatacc aaagatatct ggtcgtggac cggctacaca tgggaagaac
      961 tcatgcgccc cggagaaacc ccagacaagc tcgaattgct acatctcatc gacattctcg
     1021 tcgacggccg ctacctcaga gaccagaagg actcgctgct gcaattccgc ggctccagaa
     1081 accagcgcat cctcgatgtg cccaagtctc tggaagccgg cgaaccggtc atctgggcca
     1141 agctccacga tcaagaacga gatatccccg aaatctattt gaaagaccgc gaagccggcg
     1201 aaggccaaca ggcctcgtag tagcagttct ggaatgccgc actcagcaat atcgttggaa
     1261 ctgttgggaa aaggccttcc cgtgagcgaa atcacctcgc ctcgtgccat gttgggcagg
     1321 tggtcggact aatattccat aagaagtagc cgggcgaagc cgtccgcagg gtatctgcca
     1381 gccgtcccga aacggttcat gatgcctcaa agcgtgcact cccggaagtc ggaattaaga
     1441 ggaacacatg gttgacatcg ccactgacgc gagcgtaaag cccgcttcac acccgaagcc
     1501 cctgcgtgtg ggattggaca tcggttccac caccgtcaag gccgtggtgc ttgatcagtc
     1561 cgattccctg ggagacaccc tcttctccga ttaccgacgc caccacgcca acgtgcgtgc
     1621 caccgtggcc ggcctgttgg tcgacatcca caagaagctt gtggacctcg gacgcggcga
     1681 cgaaccgatt cgcctgtcta tcaccggttc cggcggtctg gcgcttgctg acaatctgca
     1741 tgtgcctttc attcaggagg tcatcgccga aaccgaggca atcgacaagg aatacccgca
     1801 ggccgatgtc atcatcgagc tcggcggcga ggacgccaaa atcacctacc tgaaacccac
     1861 gcccgaacaa cgcatgaacg gttcgtgcgc aggtggtacc ggcgcgttca ttgaccagat
     1921 gtccacgctg cttgacactg acgccgcagg ccttaacgag atggccaaga gctacgagaa
     1981 cctgtatccg atcgcttctc gttgtggtgt gttcgccaaa accgatttgc agccgctcat
     2041 caacgatggt gccgccaagc ccgatttggc cgcctccatc ttcaccgcag tggccacgca
     2101 gaccatcgcc ggcctggctt ccggacgccc gattcacggc accgtcatct tcctcggcgg
     2161 cccgttgttc ttcatgtccg agctgcgcgc cgcattccag cgcgcgcttg agggcaaggt
     2221 cgacgagttc atcgtgccga ccgacgccca cctgtatgtg gcctacggtt cggcattgca
     2281 ggccgacacg gattccgacg atcagggcca ccatttcgag gcctacacct gcgacgaaat
     2341 cctgaagcgt ttggacgagc tgaagaacct gccctccaac acgcccacca tgccgccgct
     2401 gttccccacc gaggccgatc gcgaggactt caacaagcgc caccacaagg aacacatcca
     2461 catcggcacc ctcgaaggcg cgcatggccc gcacttcctg ggcatcgacg ccggttccac
     2521 caccatcaag gccacgctcg tcaacgacga ccgtgaaatc gtctggtcca gctacgccaa
     2581 caacgaaggc agcccgctga ccgccgccat caacatcgtc aagaagattc agtctgagct
     2641 gcccgaaggc gcgtggatcg cacgctcctg cgccaccgga tacggcgaag gcctcatcac
     2701 caccggcctg cacttggacg agggcgtggt
//
LOCUS       DAWBMW010000202         2686 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_7539_length_2686_cov_4.737362, whole genome shotgun sequence.
ACCESSION   DAWBMW010000202 DAWBMW010000000
VERSION     DAWBMW010000202.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7539_length_2686_cov_4.737362"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          617 a          735 c          770 g          564 t
ORIGIN      
        1 catcgcgtca aaacaaccgc attaaggctg ccgcgtttcg tatcgttacg cgtaggggct
       61 ggctgaggcg taatgctgaa gagtaacatt gaagcaatgg caatcaagcc gatttgagga
      121 agagaagcac acgatggcgt actgcggcca ctgcggaaat agagttgata ggccggcacg
      181 attttgcccg gcatgtggag cgtctgtaga cagcgcaccg gttgcgtctg taccagtgcc
      241 ggccgcatcg tcagccgtac cgactgcgtc gataccggca cctgctgtgc cagcgccttc
      301 ggtaccacca tcgataccag ccgcgccggt tgccccatcg gtttacgccg cgcccgctgc
      361 cccacagccg gcaccaactg ctcccttgcc agctttgccg attcccgctc ctgccgcata
      421 tcaggcgact gatgcaaccg ccgtcctggc accgccgcgc aaatccagca agaagctgat
      481 cgccgttctc tgcgcattgc ttgcgacgat tctggtggcg gccttggcat tcatcgtccc
      541 caacttcacc gccgtcaaag cgatgctggg cttccagccc accaatccgc tggtcgtcac
      601 cttcgacgcg atcaactcgc tgtccactct gcgcagcacc aaatacgaac tcagaaccca
      661 cagcagcact gacgactccc ttgacgcgcg gatgtctggc atgtattcgc tggggcaaaa
      721 ccaccaatga ctcctccgcg tctgtgacca tcaagacaga tgaacaggca acaaccctcg
      781 cttggcacaa aggcgcattc gccgggaaaa acgtttggta tgatgacgac ccctactaca
      841 tctactaccc ggccgaccaa tatgaagatg acttaaggaa cggcgttggt agtgatgtgg
      901 cggacttgat attgaacgtc aaggatgcgt tggtcaagaa cggtgcttgg gacatcaccg
      961 gcgcggagcg aacgttctac gatcagctgg acaagatgaa cgacgatggg tactcgaacg
     1021 acatcgaccg ggccaagggc acgaaaacgt ctgccgaggt gaaggccgag gccagcaaat
     1081 tcatccaaca atatctcggc gtggagctgg aagataagaa tgtgcgcagc cagctgttcc
     1141 cgaatacatc gtctgccaag tctgccggca acacgcagct ctcgtatcaa atcgatgtca
     1201 aggcattggc ccagcacttt gtgaattact ggatcagcca tgaagcgcag tatccgcatc
     1261 tgaaatcatg ggtgattcag cagatcgagg ataccggtgt gggcgatgct gaaggcgagt
     1321 accgtcaggc gctcgacaat gtgcgcaact gggacttcgg cacggatgaa gagccggata
     1381 tgccgactat caccgtggat ctgaactacg gcaggaagcg tctgatgagc tcgattgccg
     1441 taagcgtgtc cggcaaggac gaatacggcg actcctacaa aggttcgctg tcgttgacgc
     1501 tcagccagca gaacgcggtg tctgtggacg accccgattt gactgatacg gtgagcaaag
     1561 ccaaggacaa caacgagctg gatctgtctt gagcagaatc agaatgtgcg gaacgccgtg
     1621 aacagatcat cgacgtgacg tcgcatgtga ttcacagaac gggttttggg gcttttccgt
     1681 tcgtcaggta gccgaagcat gcggattgac cgagccggcg gtcatatacc atttcaagaa
     1741 caaagtcggc ttgctgattg ccgtgctgga gcatcgtgat cgggaagaca tggctacgtt
     1801 tgcccattct cttggcgttg aacctgagga tatgtggagc ggcaacgcgc agttcggcat
     1861 tcgtgatatc tgtgctgccc ttatggagcg caatgcggct cagccagaga tggtgcggtt
     1921 gtatataccg tgctacaagg cgaatcgttg aacgagcatc atcctgccta tgagtattac
     1981 caaaaacgag aacatcgcgt gatggacacc ttaacccgag cagtcgagcg tgacggtttg
     2041 gcagatcctc gtcgcgaggc gcggactgct ttgtcgatga tgaacggcat tcgggtccgt
     2101 ttggctcagg gatcgggcat cggtcgattt agtggccgat tggaatgcct atgccgactt
     2161 ccggtggcct cgacagtcgt gaggcaataa gacttttctc gtgagatacg ctatcggcgt
     2221 gtcgtcatct ttttgttgca atacgcagaa aatgcaagaa caaaaaccct tggaatcgga
     2281 ctgattccaa gggtttttgt tgtgtctgtg gagctagccg ggttcgaacc ggcgaccctc
     2341 tgcttgcaaa gcagatgcgc taccagctgc gctatagccc catagttgaa aaaaagcggc
     2401 cgcgccgctc ttaatcaccg tgggcccggg aggacttgaa cctccgacct catccttatc
     2461 agggatgcgc tctaaccagc tgagctacgg gcccgatcca tacgcttcaa aagcgcacaa
     2521 gtgattacaa taccattgaa cggcggtatg tcaataacgg cgtgtcgcga ttgtcgcttg
     2581 cgccatgatt gccggcgcag cggagcatga cgagtgcgat gaggctgttc gcgtggtgga
     2641 agccgtaggc catgcggatg gtgaccttga tcctgttgtc ggacgc
//
LOCUS       DAWBMW010000203         2677 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_7567_length_2677_cov_2.352403, whole genome shotgun sequence.
ACCESSION   DAWBMW010000203 DAWBMW010000000
VERSION     DAWBMW010000203.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7567_length_2677_cov_2.352403"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          517 a          778 c          856 g          526 t
ORIGIN      
        1 ttcgtaccca ccggccccca gcacaaactt gagtttgccg aacaacttat ggccgacttc
       61 ccagacatga agttcattct ggtgggcgat gacgggcaga aagacccgac cacgtacgcc
      121 accatcgccc gcagatatcc cggacgtgta ctggcgatcg tcattcgtga gttgagcccg
      181 cgcgaatcaa ccggattggc gtcggtgacg gggctcacct cgacccagcc gactccggta
      241 accgatgtgc cagtattcac cggcaccacc ggctcgaaca ttctgaagac catgctgccg
      301 tatctgaaga cggtgttgcg ataggcgcgg cgtgatgaac actgaatgcg tcccggtgcg
      361 gtgcctcgcc tataatcgcg tggtatgact gacgagagca caaacgccgc ggccgcgatg
      421 ccgccttacg ccaagcgcga gccgaagcgc cgcgaattcc acggtgacgt tttcgaagac
      481 gattacgagt ggatgcgaga caaagactcc gatgatgtgc gcagctacgt agctgccgaa
      541 aaccagtact gcgagcgtcg catggcgcat ctggcggatt tccggcatac gttgtttgaa
      601 gagctgaaat cgcatgtgga agagactgat atgtcggttc ccacgcgcgt caatgattac
      661 tggtacttca cccgcacgca gcagggcaaa cagtatggcg tgcaatgccg catccccgtg
      721 cgcgatgccg atgactggga cccgccggtc gtggatccgc aaggcgcgcc cggctccatg
      781 cccggtgaac aggtcgtttt tgatgcgaac aaggaatccg aggggcatga cttcttccgg
      841 ctcggcggct tggatctgag caaggacggc cgttggatgc tgtacggcgt ggatgttgcg
      901 ggagacgagc gatatgattt ccgcattcgc gaccttgccg ggctggagac cggggagccg
      961 gtcagcgaac tgcccgaaca gttcaccggc attggcagcg catgcttcac gccggacggc
     1021 caatgggtgt tctgggtgga attggacgat tcctggcggc cgctcgctgt gtggcggcat
     1081 cgtgttggca cggcggtcga atcggatgtg tgcgtgtacc gagaaaccga tgaacggttc
     1141 tgggtgggcg ttggcatcag ttttgatgag cgcaatatcg tgatcggcac cggttccaaa
     1201 accaccaccg aagtgctgat gctgccggtg gccacgcctg aaggcgaatt ccaagctttc
     1261 attccgcgtc agaacgatat tgaatatgat gtgagctttg cctgttttga aggcgcgggg
     1321 gagagcggtg cggatgtgcc actcgccgtg gtctatcaca acgctgccaa tccgaatttc
     1381 gaaatcgacg tcattgacat gcgtggccat gagccgccgt accgactcgg cgaaggcgtg
     1441 tgcattgccg ccgggtcgcc gtacggctgt gaacatggtg agccggataa gccgattacc
     1501 gaggcatact tcaacgccgt caatccggcg atattgcagg gtgcccacgg tctgggcatc
     1561 gaaggcatcg cgttgcatcg caactttgtg tccctgagct accgtgccga cggccagccg
     1621 cacgtggcat acatgaccaa gagcgcggct gccgccgact tcctggccgg acggccgtgg
     1681 cggtttacgg aattgctgcc gcctgccctg gaggacgact gggacatggt tgccgacgat
     1741 cgcgaaaact tcaccggcga tcatggcgaa atcttgtgga ccgaagatga ggcccccacg
     1801 gatcacacgt catcttccga tggtgctagc gacgaccacc tgcccggtga gacccggcgc
     1861 ttgtacacca tcggcgtcgg cggcaacccc tcgtatgagg cgccgcgcat gcgctactcg
     1921 ttcgccagct acacccgccc gggcgagttg cacgaaatcg atcccgccac cggtgaagat
     1981 gcgctgctga agcgagccac cgtgttgggc aacttcaatc cacgcgatta catggaacgc
     2041 cgtgtgtgga ttaccgcgcg cgacggcgaa cgcattcctg tttccctcgt ctggcaccgc
     2101 gattgcccgg cgcaggattc gccgatgttc attaccggat atggtgcata cgagatttcc
     2161 tccgatccgg ggttctcggt ctctcgtctg agcatgttgg atcgcggtgt gctgtacgcc
     2221 gtgccgcaca ttcgtggcgg cggcgaaatg ggacgcgcct ggtatgagca ggggcgcaga
     2281 ctgaacaaga agcattcctt tgaggacttc gtggacgcca cacgcgcgct gcagaaggcg
     2341 catttggctg atgcttggcg caccgtggcc aacggcggat ccgctggcgg cctgctgatg
     2401 ggcgcgattg ccaatatggc gcccgaatgc tacgccggtg ttgaggcgga tgtgccgttc
     2461 gtggacgcgt tgacctccat tcttgacccg gacttgccgt tgaccgtcac cgaatgggac
     2521 gaatggggag acccgttgca tgaccctgag gtgtatcagt acatgaagtc gtacacgcca
     2581 tacgagaatg tgccggtcgc cgtgctcgag ggcggcaacg acgaggctgc cggtgacggc
     2641 agtgctgatg gggcggccac cggcgcggtc gccgccg
//
LOCUS       DAWBMW010000204         2665 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_7610_length_2665_cov_2.509579, whole genome shotgun sequence.
ACCESSION   DAWBMW010000204 DAWBMW010000000
VERSION     DAWBMW010000204.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7610_length_2665_cov_2.509579"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          527 a          759 c          799 g          580 t
ORIGIN      
        1 ttcgcatccg ggcggaacag ccaggtggag ccgtcggcat gacggtaagc cttgaggccc
       61 tcgaagcact cctgtgcgta gtgcagcacg gaagcacccg gatccatctt gagcggtgcg
      121 tacggttcga cgcggcggtc ggaccagcct tcgcccttgg tccaggtcat gtgggtcatg
      181 ttgtcggaga acacctggcc gaaagccggc ttgtcgatga gggactgacg cttctcatcg
      241 gatgcggggt tgtcgttcgg cagtacggtg aacggttcgg cgagcttgtc gagcgctgcc
      301 ggatcgtggt gcgtgttttc agtcattact agctacttct tcttgacttt ggcggcccga
      361 aagccaccat atggggtgtg tgctgtgcgc ttgtggcgcg agctctgttg catacttttg
      421 cacacccatg tgttactcgt gtggcagagt tcacatggtg aaaaacgaag aagcccgcac
      481 cgttctcaac ggctgcgggc ttccacgttt cagttatgtt ctgaattgtt cgctatcgaa
      541 gcgatcgact cacttggcgt cggcagccgg agcagcagcg tcggcagccg gagcagcagc
      601 gtcgggagcg gcggtggcct cagaagcatc ttccggcacc tcaacggtga cgacggactc
      661 ttccgggtcc tcaacgtcga gctcaacgcc ttcaggcaga acaacgtcct tagcgaacac
      721 cttggtgccg tcggttaggc cctcgacgct cacaacgatc ttctcaggca agttggcgac
      781 gtcagcgcga accttgagct cctgaatgtc gacgaatgcc acagcagcgc ccttcggggt
      841 gccttcaacg aagaccggca cctcgacgtc gatcttctcg ccggccttga cctcaaggaa
      901 gtcaacgtgc tcgatgatgc gcttgaccgg gttcttctgc acgtccttga cgacggccat
      961 cttggtgttg ccgtcgaagg cgatggtgaa cagggcgttg gtgtgacgca gggccagggt
     1021 ggtctcgcgc atcggcaagg tcacgaaggc gggctcttcg ccgccggcgt agatggtggc
     1081 cggaatcttg ttggcaacgc gcagacgacg agcaacgccc ttgccgaact cggaacgggc
     1141 ctcgccctca agcttaatgg tggtagccat aatggatact ccttgtagtt tcgtttcgtt
     1201 ctgtctcgct cagacgccac gacacgccga aagacaccct acaaggagtg aaatacaccg
     1261 agtcgataac ggaagctgtg tcatgtgtca gcacgcacac ttccctcgcc aaagcaacaa
     1321 ttgtcaagta tacgccaact ccggactttg gcgcggtgat gcttcgatga cactgcatcc
     1381 cggccgtccc agccacgtca acgtacggtt tccgcgaaat aatccctaac tttgtgaaga
     1441 acgtacgctt acccgttctc aacgtacggt tttcacgaaa tcgtccgcca tttcgtgaaa
     1501 atcgtacgct cagcgacact cagtgtacgg tctttcacga agttggggat gatttcgaaa
     1561 ataacgtacg atggtgagta ccgtatcggg aggttgatat gactcgttcg gacgacgtca
     1621 tcaatgagga tggccatgac gtcatcgccg cacacactcg cgttgtcagg ggtatggctg
     1681 cggcacttgc cgcgatagcc gccacaggag ccggcgtcac agccgcggcc gccaacgtca
     1741 tgttcacgtt cgcgctggac accaaggcca aacgttcgat gttcaacatg ccgcacgagg
     1801 agacaccgaa aggcatcgaa ttcgacatgt ccgagcagct tgaggccgcc cggtggttcg
     1861 aggaggccaa gcaatcggtc acgctgcgca gccatgatgg attgaaactc cacggctggc
     1921 tgttggaccc ggattgttcc gacccacagc cgcatctata cgcgatctgc tgccatggct
     1981 atgccggaga gcccgcggaa atggccaaat gggcgcatcg ctatgcgcaa ctcggtttta
     2041 cggtcctgct acccgcgcag cgcgcccatg agttgagcga agggcgttac gtgggtatgg
     2101 gattgctgga aagcgacgat ctgttgggct gggtttcgct gatcaccgca gctgatccgg
     2161 atgcacgcat tctgttgcat ggcaattcga tgggcgccgc cacggtgatg atggcggccg
     2221 gcgatgcgcg actcccccgc aacgtcgtcg ccgcgatttc cgattgcgga tacggttccg
     2281 tggtctcgca gtttacggat aatgccgagg cgatgttccg tttgccgcac tcgttggccg
     2341 tgctgctggt caaagtggcc agtcatgtgt ctgaacgcaa ggctggatac cggtttgagg
     2401 acgcctcctg tgtgaaggca ttacggcacg cgactattcc gatgatgttc attcatggcg
     2461 gagcggatac ttttgtcaat ccgaagtatc tggacatcaa ttacaacgcc tgcgccagca
     2521 tcgaccgcga gaagctgctg attcccggcg ccgaccacac gatgagcgcc agcaccgatc
     2581 cggaccgcta ttggcgtcgc gttaacagct ttgttaagcg ggtgtttggg ctgtagtaga
     2641 tatagagctc ggctcccctc tgtga
//
LOCUS       DAWBMW010000205         2653 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_7653_length_2653_cov_1.998845, whole genome shotgun sequence.
ACCESSION   DAWBMW010000205 DAWBMW010000000
VERSION     DAWBMW010000205.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7653_length_2653_cov_1.998845"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1435..1444
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1835..1844
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          601 a          780 c          752 g          500 t
ORIGIN      
        1 ccaaggagcg caagcaccgc atcgaagatg ccgtgcgtaa cgccaaggcc gccatcgagg
       61 aaggcctgct gcctggcggt ggcgtggccc tcgttcaggc tgctgccaag gccgagaaga
      121 ccgaggccgt cacctccctg accggcgaag aggctaccgg tgccgccatc gtgttccgcg
      181 ccatcgaggc cccgatcaag cagatcgccg agaacgccgg cgtgtccggt gacgtggtca
      241 tcaacaccgt ccgctccctg cctgatggcg aaggcttcaa cgccgccacc gacacctacg
      301 aagacctgct ggccgccggt gtgaccgacc cggtcaaggt gacccgctcc gctctgcaga
      361 acgccgcctc catcgctggt ctgttcctga ccaccgaggc cgtcgttgcc aacaagccgg
      421 agccgaagtc tgctgccccg gccgccggtg ccgacatggg ctactgatcc gcaaacgatc
      481 gctagctgat tgagctgaaa ggggactcct tcgggagtcc cttttttgta ttctggctcc
      541 cctctttgag gggagccaag aaactagctg gcaaacaggc gcgaagcttg gatttcggcg
      601 tcagcgtaca cggtggaggc ttgcgtcagc gagcgttgga tggactcgag tgaggcctcc
      661 atctgctgtt gggccgcacg ccactgttcg gctaccgcgg tgaactgggt ggccgccgaa
      721 cctcgccatg cgtcctgcaa ggcattgaga ttggtgtaca tgccgcccac ggcctgcctg
      781 atttgcgaga tcgaagtggc cactgcggcc gaggatgatt ggattcgctc ggaatctacc
      841 tgatattggg gcatcgttgc tccttttcta gagggttaat ggtatgtatg gcggccgatt
      901 gccaataccg gccgttaagg tggaggatat gtcaacaacg aatcgggaag acggaaaaac
      961 aactattgtg gtcctagctt cggagccgtc ctattcggcc ttcgggaata tggtccgaaa
     1021 atggcttgtt tgcgccattt gcgcccttgc tgcagtagcg atgaacgcaa tgtggataac
     1081 tgcaaatgcc tatggggata actcgagcga cagctctgac agctccagca gcagcaccga
     1141 cagcggcgtg accatcaccg agaacatcac ggatacggaa aatctcctgg gctcccatgc
     1201 ggccgaagtc acggatgcca tcgccaaaac cgagaaggaa accggtgtac acgtgcatct
     1261 gctgtatctt tccagtttca atagtcaaca gaaaccggga gactgggcgg caaccgtgat
     1321 ggagtccatg aatcccaagc cgaacacagt gatgcttgcc gtggcctcga atgacggcaa
     1381 cctggtggtc gtcgtgtcca agaactccga taagtggctt ctcgataaca aaacnnnnnn
     1441 nnnnaacaaa accgtcgata agctatccga agccgcacag cagccgttac tggaaaaccc
     1501 gccgagctgg tctggcgcgg caacggcaat gatggatcaa atcgtgaaat ccaaaaaagc
     1561 ctccacctca tcgtccacgg tgatcgtagg cataatcatc atgggtgtgg tattggtggc
     1621 gctggtcatc atcatcgtgg tcatggtggt gattcatcgg cgcaaggaga tcaagaagga
     1681 ttccaaggcc gaactccaag atgacattca ggaaacgccc agaagagcgc gacactctag
     1741 gaagcatgag taagcctatt gaagcatcga ttgttgttgt cgacgacgag ccgtccatcc
     1801 gagaactgct ggtcgcttcc ctgcacttcg ccggnnnnnn nnnnaagcca ttgaggttat
     1861 cgaaaaagtg cagcctgacc tcatcgtgtt ggacgtcatg ctgcctgaca tcgatggatt
     1921 caccgtcacc cgtcgcatcc gccaggaagg cattaacgcc cctgtgctgt ttctcaccgc
     1981 tcgtgacgac acccaagaca agatcatggg cctgaccgtc ggcggtgacg attacgtcac
     2041 caagccgttc agcctcgagg aagtcgtggc tcgcatccgc gccattctgc gccgtacccg
     2101 cgaacaggtg gaagacgatc cgattattcg cgtcgccgac ttggaaatca acgaggactc
     2161 ccacgacgtg acccgtgcag ggcagccggt tgacctgagc cctaccgaat acaagctgct
     2221 gcgctacctg atggacaacg agggccgagt actgtccaag gcacagattc tcgaccatgt
     2281 ctggcaatac gactggggcg gcgacgcagc catcgtcgaa tcgtacatct cctacctgcg
     2341 caagaaagtc gccggcatcg aggtcgacga cggcgaaggc ggcaagcgca aggtgactcc
     2401 gctgatcgaa accaagcgcg gcatcggata catgattcgc gaaccgaaga actaatcgcc
     2461 tccatccata acgtatcgca atctcatgag tatccccgat aagcagtcag gcggcgtacc
     2521 gccgagcagc acgccgaacg ccgccccgaa cacgaatccc atgcctgcaa tgtcgccgtt
     2581 cgccgcccat ttccaagagc agcgcgagcg taagaaaaac atgctgatgc ggcatatcga
     2641 ccgtatctcg ttg
//
LOCUS       DAWBMW010000206         2642 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_7687_length_2642_cov_2.441825, whole genome shotgun sequence.
ACCESSION   DAWBMW010000206 DAWBMW010000000
VERSION     DAWBMW010000206.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7687_length_2642_cov_2.441825"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          495 a          928 c          738 g          481 t
ORIGIN      
        1 acatcgactc ctccgacttc ctcggccgac tcggtctggt ccgtatctac aacggcaccc
       61 ttgagaaggg caagacctac ggcctgtccc gtgtggacgg ctccctcgag aacttccgcg
      121 tctccgaact gctgcgcacc cagggcctcg agcgtatccc ggtcgaatcc gcaggccccg
      181 gcgacatcgt ggccgttgcc ggcgttaacg acatcatgat cggcgagacc atcgtcgacc
      241 ccaaggacca caagcttacc gcccgcatga tcaaggaccg tctggaccgc gagctcatcg
      301 gtaacgtgtc catcaaggtg ctgcccaccg accgtcccga cgcttgggaa gtccagggcc
      361 gtggcgagct ggcgctggcc gtgcttgccg agcagatgcg ccgcgaaggc tacgagctga
      421 ccgtcggccg tccgcaggtg gtcaccaaga agatcgacgg cgtgatcaac gagccgatgg
      481 aaaacaccac catcgacgtg ccggaagagt acatgggtac cgtcacccag ctcatggccg
      541 accgtaaggg ccgtatggat ggcatgacca accacggctc cggctgggtc cgtctgcagt
      601 tcaccgtgcc gtcccgtggt ctgatcggct tccgcaccgc gctgctttcc gccactcgtg
      661 gcaccggcat ctcctcctcc atctctgccg gctacgcgcc gtgggctggc gccatcacca
      721 ttcgccagaa cggttccatg gtttccgatc gcaagggtat cgccaccccg tacgccatgc
      781 agcgtctgca ggcccgtggc aacttcttcg tcgaaccgca gtccccggtg tatgaaggcc
      841 aggtcgtggg tgtgaacaac aagcccgacg agctggacgt gaacatcacg ctggccaagc
      901 acatgaccaa catgcgttcc tccaccgctg acgtgctcga aaccctgact ccgccgatca
      961 agatgagcct tgaggagtcc ctggacttcg ccaacgagga cgagtgcgtg gaagtcaccc
     1021 cggagtccat ccgcgtgcgc aagatcatcc tcggccgtga ggactggtac aagtggcgcg
     1081 ccaagcagcg ccgccagaac gccgcccagc agggtaagtg atttggtgcg gctgtagccg
     1141 accattctct ctagctcccc tctctgaggg gagctaagtt tattcctcac caagtcatat
     1201 ccccacctat actgaaacgc atgactgtga agagcactac tgaattgcgt ccgtggtcgt
     1261 atcgccagaa cgcgctggtc aaatcgttga tcacgattgc ggcgggcgtg gcttccgcct
     1321 ttgtgggcac gttcgcccac cgtatgggtg cggaactgaa tattccgtat ggtctggtgc
     1381 tggcgtttct gctgatcggc ctatcgacat ggtgcgcccg ttcgcgcatg ggcgcggtcg
     1441 gactggccct gcacctcatc gcctcctcac tgaccgcatg gggcatggcg ctgaccacca
     1501 catccggcaa agcgctcatc gtcgccggat tccaaggcga catgccgttc ttctcccaac
     1561 atgccggcta catctggctg tacggcctga tactggtcca agtcgtcctg ctcattcttc
     1621 ccgcccgctg gttcgtcatt cccactcatt ctgaatcgcg cgcgtaacta tatatggcga
     1681 tacattcccg ggaatgcgtc gcgtatcggc gggcgtaggg gaatacggcc gctaccatag
     1741 aactttatgg cacgcatgaa actgttttac ttgggcccgg agggcacctt cacccatcag
     1801 gcggcaatga cggccgccga ccagttcgcc acactcggcg acttcgacct catcgctctg
     1861 cccgacgtgc ccgcgatcat gcaggccgta cagtcgcgcc aaggctgggg cgtcatcgca
     1921 tgggaaaaca acgtcgaagg ctatgtggtc ccgaatctcg acgcgctgat cgacgccccc
     1981 aacgcggccg gcttcgcccg gatcagtgtg gacgtggcct tcaacgcctt caccgtgcgc
     2041 ggccacagca tctacgactg caccggcaac cccgtcagcg cccacccgca cggtctggcc
     2101 caatgcaccc ggttcatcgc cgaacaccat ttgcagcccg aacccgcatc ctccaacgcc
     2161 gccgcctgcc gcgacctgaa gcccggccgc gtggcgctcg gccccagcat ctgcggcgaa
     2221 ctctacgatc tcgacaccct cgccgaacat gtgcaggatt tcgatggcgc gcacaccgaa
     2281 ttccttgtca tcgccccgcg tgatgtggtc caggaactca acgcccgcgc ccacagcaat
     2341 gacgccggcg acttcgaaac catcatcacc ttcatcccgc tggtcaccgg ccccggcgta
     2401 ctcgccgacc tgctggacgt cctgcgcgat gcgggactga acatgaccag cttcatctcg
     2461 cgcccgatca aaggccacga cggaacctac agcttcatcg ccacccttga cgccgccccc
     2521 tgggagccga gattccgcac cgcactggaa gagatcgccg gacacggcga ctgggcaaaa
     2581 accctggccg tctacccacg ccgcgaacgc ccgaacccgc cggtcgacgc ctggatgctg
     2641 cc
//
LOCUS       DAWBMW010000207         2632 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_7720_length_2632_cov_2.365153, whole genome shotgun sequence.
ACCESSION   DAWBMW010000207 DAWBMW010000000
VERSION     DAWBMW010000207.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7720_length_2632_cov_2.365153"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          561 a          745 c          796 g          530 t
ORIGIN      
        1 ggttaagcct cttggccagc aactgcgcat gctcggcctg gtcgttgatg ccgcgcatca
       61 acgcgtattc gatactgacg cggcgcttgg aggccagcca ataatcgtgc gcggcatcca
      121 acacctgctt ggtattgaaa cgcttgttca tcggcaccag ctcgtcacgc agttcgtcgc
      181 tgggggcatg cagggaaacg gccagacgca cgggaatgcc ttcggcagtg agcttcttga
      241 tgccggggac cacgcccacc gtggatacgg tgatgttgcg cgcggaaatg ccgaagcctt
      301 cgggaggcat ggcggaaatc tgtcgtacgg cggacagcac ggagttgtag ttgcccatcg
      361 gctcgcccat gcccatgaac acgatgttgc tcaggcggcc ctctcccccg gccacttcgc
      421 cgtcgcgcat catcttggcg gccacacgca cctgttcgat tatttcaccg gtggacatgt
      481 tacgggtcag gccaagcttg ccggtggcgc agaacgggca gtccatgccg cagccgacct
      541 gcgaggagat gcacagcgtg gtgcgggtcg ggtatcgcat aagcaccgat tcgatgaggg
      601 agccgtcgaa cagcttccac aaggtcttga tggtggtgcc ttcgtccgcc acttggcggg
      661 tcacctcggt gatgagctgc gggaagaacg cggcggcggc ctcgctgcgc ttggctgccg
      721 gaaaatcagt gaattcggcg gcattcacat cgaaatggcc aaagtaatgg ttggcgagct
      781 gcttgactcg gaacttcggc aatccgagtt cggccgcttt ctcgatgcgc tgctcgggcg
      841 tcatatccac aaagtgcagc ggcggcttac cacggcgggc atggtccttg gatagcacgt
      901 cacggaaggc acctgatgtg ccgccgggag taatgccggt ttccggagtg tcaggatgct
      961 gtgtggtcat gatgggttcg cttcgctctc aatatcagaa aatagtcaaa catgcaatag
     1021 taatatcaga atccaccata cggcgtgccg catatgaatc agatggccca tgtcttagcc
     1081 catagcgaac gcatattgaa cgatgatttc cccatatatg gcatgtggca ccgtgcgata
     1141 tacacacgat gccacattgt tcatgcacga gacatgcgaa tcacagtccg gtgatccaca
     1201 gcaaggcgca ggtgaacggt gcactcatca aaatggaatc cacacgatcc atcacaccgc
     1261 cgtgcccctt gagcagatgg cccatgtctt tgataccgat atcgcgcttg agcatggaag
     1321 cacacaaatc accgaacgtg cccacggcac cgatgagaat acccgccacg atcggcaccc
     1381 accaacgagt ggcccatttc gaggcatcat acgtgcatgc gaatacggca aatgcgccag
     1441 ccatggcgaa gagcatcgag ccgacaaggc cctcaacgga cttcttcggc gagatgcgcg
     1501 ggctgagctt gtgtttgccg agccaggcac cggcaaacaa accgcccgta tcggataggg
     1561 cgggtaggaa caccagcatg atggcgtgtg caatcggatg cccattaaaa gtcagtgaga
     1621 tgatgacgca gctggccaac aacggaatat acaacacggt aagcacggac acagccacat
     1681 ggctgagccg ggaatgatgc tgttccccgc cctcatgatt aaacgaggat tcgaggcgcg
     1741 cgctggcatc cgtgttggac agtttgccgg caaccgccag agataggcgg ttgccgacgc
     1801 tgatttttgc gctggccgcg atggcgacca gaataatcga cacgatgata ctcagcgaca
     1861 tcgtgatgag atggtacggg gaatagtagg tggcaatgag ggtgaaggac gagcacagcc
     1921 acagcataaa caccgggagg tgcaggccca cggtggcgaa gtccaccctg agctcccata
     1981 gcgccagaat catgaacacg actaccagca gcacgaagag gtcgacgctg agcagcaggc
     2041 acgcgacaat aaggatgacc agtgccgcgc ctgtggcgat ggcctgcggc atgttgcggc
     2101 cggtcttctt gttgatctgg tcgagcgtct cctcggcctc ttcatggggt tgttcgttgc
     2161 gttccataat tctccggacc tggtctgaat cagacctcca tgatctcctt ctgcttggtc
     2221 tccagcagag tatcgatctc gtcggtaacg gacttggtga ccttgtcaag atccttcagc
     2281 aggcgatcgc cttcgtcctc acccatctcg ccgtccttga cggccttgtc gatggtttcc
     2341 ttggtcttac ggcgaatgtt gcgcacggcg accttgccat cctcggcctt gcccttggcg
     2401 agcttgacgt attccttacg acgatcctcg gtgagttccg gcatggtcag acggatgacg
     2461 ttgccatcac ggttcgggct gatgccgaga tcggagttgc gcagggcctt ttcgacggca
     2521 ccggcctggg aagcgtcaaa cggggtgacg gacagggtgc gcggttcggg aacgccgatg
     2581 gaggcgacgg ccttaatcgg ggtgggggca ccatagtagt ccacggtgat gc
//
LOCUS       DAWBMW010000208         2626 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_7745_length_2626_cov_2.881758, whole genome shotgun sequence.
ACCESSION   DAWBMW010000208 DAWBMW010000000
VERSION     DAWBMW010000208.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7745_length_2626_cov_2.881758"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          656 a          806 c          649 g          515 t
ORIGIN      
        1 ccatggaagg ccgctcaaac ctcaggtgtt gcaaccacca ctagaatccg cctgacacat
       61 ttccctccac aatcgaacac tagtcattca cgaaatccat cactacgagg catcgcaatc
      121 gaacggcatg caatatcaag cacacaccac aacaacaggt cgtacaaata ttgcctttac
      181 atcagtccgt ttcggacatt atggtcacag caggctctgc gacaactctt caagagacat
      241 ggcaactaca tgaaactcga atacggcacc gcgcgaagca agcgccctcg ccacattgaa
      301 agcgaattca gcacgagcgt cctcgttagc gatacttgcc gccgtggcga cgaagaccag
      361 ccatctccgc ccgaccagca cacccctttt ctcttggtca cagcgccatt gagccttact
      421 cgtacgatga tgatcgccat catattcaac agcgaccttg aactctggcc acgctaagtc
      481 caacgttatg ggagctccag atgcaaaagc cgcgtccggc accacatagt tcgcaaccgg
      541 ccgtggaatc ccatgcgcta ccagagagag tcgttcttct gattctttgg gcgaatcagc
      601 accaggtgat atcagaggca atgctcgaac gcaggacggg cgccctctga atcgggtaaa
      661 ttgctccacg aacctcacca gatcctgata gattgcagca gcatcacgat ccttggccaa
      721 tactggctgt ttgctcgtag cagcgatgac ggcatccccg agcacaatca gggactcaaa
      781 agaaacatac ggggcaagtt gggcccatac gtgaaacagg tccagcgcaa acacgtgttg
      841 gttcatttcc acattggcgg ctttagccgc atgtttccaa atatgcgttt gcagtggtgc
      901 atggcgtgta cgaaaccgct tgctcttaac gctggataca gtgtgcagct tggccgagtc
      961 gaggtcgcaa tcggcgggaa tcggtacgga ttgcagagcg agggaagtgg tcatgccgaa
     1021 aagcagcttc ttgcccgccc gtttcgcttc ctcagtgcag cgtgcgacca tgtcgcgtct
     1081 gcgctgggcg aggctttcgc tcatggtcgg tgtcgctgca gaatcatgtg caccactgtt
     1141 cggcgatgac gagtcgcgag aagtcgggta tttcgagtca gtagaaaatg accccgcaaa
     1201 ttcaggcgga acaaaaaccg cctccgattc ttccggaggc tgatcgtata tagagcctgg
     1261 cagagctcca tagttggtga tgccgtcggt catcctgttc atgacaccac catatgggta
     1321 gtgggtgcgg aaacggaagc cgatccgagg tatgtggtcc tagcttcact tacgcacatt
     1381 cattgtggat aaccatgtgg acacctcact tatacacata gtcatccacc tcttcaaccc
     1441 cgatttgaca cactaactgt atctgatatg attacagtat cataactgta cacatcatag
     1501 ttacagtatc gactcggtaa ggaggtcgtt tcatgaacgg cgaccattca ctggcagtgc
     1561 acgcattggt ctaccttgac catcgcgcca cacacctgcc cagtcagatc ctggccgaga
     1621 acatatgtac caatgcggcg cgcgtgcgca aagtaatgcg accgctcgcc tcagccggac
     1681 tcatcgccac taaagagggt gctgaaggcg ggtacgctct ggcccggccc gccgccgaaa
     1741 tcactttgcg cgcggtagcg gaagccaccg gcaccacttt tgtgaaagtg aactggacac
     1801 cgggcgacgt acacgaggac tgtctggtgt catcttcaat gggcgcagtg atgaacgaca
     1861 tctatgccgg gctcaaccgc acttgcctcg aacaactcga acacaccacc atccacgaca
     1921 tcaccgaaca gatattcagc gagcgcaaag gctaataatc ccgcgcgttt cccctagcca
     1981 gcatcagtcg ataacgcttc ggcagtcaaa gaagaaaaca gacgccgcac ggcacacaac
     2041 acatcagcac aacgcactct tatcactcga atacgtaagg aagtaaccat gtccatcagc
     2101 acacccaatg ccatcagcac acccagcacc tcccccacta tcaccaccga cgccctgatc
     2161 attggattcg gcaagggcgg caaaaccttg gctgccaagc tcagtgcagc cggccgcaag
     2221 gtcgtcgtcg ccgaggcaag cgccgacatg tacggcggca cctgcatcaa tatcggatgc
     2281 ttgccgtcca agtcactgat tttgtccgcc gaacaggcgc ggcgggatgg cgcgaacagt
     2341 actcccgaaa cccgcgaagc agcgttcgag gctgctatca aggagaagcg ccgcgtcact
     2401 tcgatgctgc gcgacaagaa ctatcacaag ctcgccgacc aagacaacat caccgtaatc
     2461 accggccgcg ctcacttcac cggccctcat agcgtggaaa tcgccaccgc tgagggcccg
     2521 gtcgccgtga ccgccagcaa gatcttcatc aataccggtg ccacgccgcg cattccggat
     2581 attcccggta tccacactac gcccggcgtc tacaccagca ctggac
//
LOCUS       DAWBMW010000209         2616 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_7779_length_2616_cov_1.944553, whole genome shotgun sequence.
ACCESSION   DAWBMW010000209 DAWBMW010000000
VERSION     DAWBMW010000209.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7779_length_2616_cov_1.944553"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          568 a          834 c          755 g          459 t
ORIGIN      
        1 cggacaagca gcgtgggcag gccgaatccc tgtatgagcg tttgcgccgc catgtcagcc
       61 cgggccagac cccgtggctg agcctgaccg gctatgagct ggagccccga agcttctcca
      121 tcgtcaagcg cgcgatcaac accgattcgc tgttggacat ggagtactcc gacggtaccg
      181 gtagtacccg tcgcaagctg gtggcccccg ccaagatttt catcgacgaa ggcgtgtatt
      241 atgccgccgt ctacacggat gtcggttccg ccgcccctaa ggacaagaag gcctacgtct
      301 ccaaagacaa gaccatcgac aaggcgaacg gcaagccgcg catctggcag gtgctgcgct
      361 tggcccgtat cgaaaaggcg gaactggtca agcccaccaa gcagatcaac atcccgaaca
      421 tgccggtgag cgaactgcgc aagtggagtt tcgacaacgg taccgacgcg gtgttcatca
      481 ccgatcagcg cgatctggca ttcatcaaga cattgccggg cgctaccgtc gaacaatgcg
      541 gtgccggtga aaaggtgcat ctgaccgttt cctccgactc ctggttcgtg gccttctgca
      601 tcgcacacgc gcgtcatatc acggcggtcg ccccggaaac gttgcgcacc atgatcgtgg
      661 cacgcgccga acgcgaactg agcatcagcg agaacaagta aggagccttt catgccttgg
      721 tggatatggc tgattctggc cctgttcatg ctggccatga ttgttgccgg actcatctat
      781 gcagcccttc atggaatccg tgccttgaaa gacatgtccg acatcggcgg ccagctcggt
      841 gaacgcgtgg ccgcgatggg ggagtctggc acctctgccg acaccagcga gccgcctctg
      901 ttcactcagc cgctcagcgt ggcacagaat cgttatgccg aggcccatgc ggacgtgatt
      961 cgcagcaagg ccgccaagcg cgagcgccgc acccaggcac gggcccgctg gaagcgattc
     1021 aacaactaac ggaaagcagc aatggcacgt caccaacacc ataagggcaa tgccgattcc
     1081 cagacgctga gcccttcgca gcggtacgca tcattccaga aagtccaacg ccatcgggcg
     1141 tccgcagcag cgcgattcgc cgaatccctg cctttcgaac tggacgattt ccagaccgaa
     1201 gccaatgacg cgctcgaagc cggcagcaac gtactggtcg cggccccaac cggtgccggc
     1261 aaaaccgtag tggcggattt tgccatttac ctcgcccagg aacgcaacgt caaagccttc
     1321 tacaccacac cgatcaaggc gctgagcaat cagaaatacc atgatctggt cgacgtgtac
     1381 ggcccggaca aggtaggcct gctcaccggc gacacctcga tcaactccga ggcggacatc
     1441 gtggtcatga ccaccgaagt gctgcgcaac atgctctacg agcgttccac cacgctgaac
     1501 gcactacgct acgtgattct cgacgaagtg cactatctgg ccgaccgatt ccgcgggccc
     1561 gtgtgggagg aagtcatcat ccacctgccc aagacggtca agatagtcgg cctgtccgac
     1621 accgtgtcca acgtggagga tttctccaac tggattgcct cggtgcgagg cgacaccaaa
     1681 ctggtgatca gcgaacaccg ccccgtgcct ttggaacagc acgtcatcgt gcaggcggac
     1741 gaatacactg agcccgaggt gctggatctc tatcgccgcg acggcaacgg cgagcaggcc
     1801 accaagctca acgccgaact catcaaccgg ctcgaccagt tggaccgcaa ggccgcacgc
     1861 cggcgcggcg aggaacgtcc cgataagcgc aagggcttcg gcagaggccg cggcggcaaa
     1921 ggcgccaagg gccatgcccc gaaagcggaa cgccacaccc cgcgccgctg ggccgtggtg
     1981 gacgagctta acttcctggg aatgttgccc ggcatctact tcatcttctc gcgcaacggc
     2041 tgtgaccagg ccgtggaaca gtgtatcaat gctggactgg agctcaccac cgatgaggaa
     2101 gtgaccaaga ttcgccgcat cgtggatgag atggtggagg ggcaactcac tcaggaagac
     2161 ctgaaagccc tgcagttctc caagttccgc tttgcgttgg aagaaggctt cgcttcgcat
     2221 catgccggca tgattgccct gttccgccag atagtcgaac gattgttcga agaaggtctg
     2281 gtcaaaatgg tgttcgccac ggaaacactg gccttgggca tcaatatgcc ggcccgctgc
     2341 gtggtggtag agaaactcga gaagttcgac ggtactggtc atgtgggcct gacgcccggc
     2401 gaattcacgc agctcaccgg tcgtgccggt cggcgcggca tcgacaccat cggccacgcc
     2461 atcgtggtcg accatcacgg ttttgtgccc gccacggccg ccgcgctgtc atccaagcgt
     2521 gtctacccgc tgcactcgag cttccgaccc acgttcaaca tggccgtgaa cctgctcaac
     2581 tccagcgact acgagaccgc gcacgttacc ctggac
//
LOCUS       DAWBMW010000210         2614 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_7783_length_2614_cov_2.299336, whole genome shotgun sequence.
ACCESSION   DAWBMW010000210 DAWBMW010000000
VERSION     DAWBMW010000210.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7783_length_2614_cov_2.299336"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    376..385
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2095..2104
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          493 a          724 c          778 g          599 t
ORIGIN      
        1 gtggatgatg ttggtcttgt cgagacggta ctcgatctta ccggctttgg cttccttgat
       61 ggcgttggca atgttcggcg tgaccgtgcc ggccttcggg gacggcatga ggcccttggg
      121 gccgagcacc ttaccgagac ggccgacaac gcccatcatg ttgggagagg cgatgacggt
      181 gtcgaactcg aaccagttct caccctggat cttggccacg agatcctgac cgccggcgta
      241 atccgcaccg gcgtccagcg cctcctgaac ctgctcttcc ttgcagaaga cgagcacgcg
      301 ggtgaccttg ccggtgccgt ggggcaggac gatggcgccg cggacctgct ggtcagcgtg
      361 gcgggagtca acgccnnnnn nnnnncgtcg aacttggcct tagcggtctt ggtgatgagc
      421 tcgaaggctt cctgcggctc gtactgagcc tgcttatcaa tgagcttagc gctctcttta
      481 taatttttac ctctgaacaa ttcagtttcc tcctaataaa gtggttcgtc ggatggatga
      541 tcctcccacg atcagggagc ggaatctgac ggcttattcg ccgaccagaa cgcccatgct
      601 gcggcaagtg ccggcgatca tgctcatggc agactcgatg ctggagcagt tgagatcggg
      661 catcttctgc tctgcgatct tgcgcagatc ttccttgctg atggtcgcga ccttatctct
      721 ctggggcacg ccggaggcgt gatcgatgcc gcaggccttc ttgatgagca gagctgccgg
      781 gggcgtcttg gtgatgaagg agaagctacg gtcggagtac acggtgatga cgaccgggat
      841 catcatgccc atgtcgccct tggtgcgctc gttgaagtcc ttggtgaact ggccgatgtt
      901 gatgccgtac tggccaagcg cggggccgac cgggggagcc ggcgttgcct tgccggcggg
      961 aacctggaat ctgacgtatc caactatttt ctgtgccact tgaaagcacc tctttcttgt
     1021 aagattttaa tccttgattg cttcgacctg atcaagctcg aggtcgaccg gcgtctctct
     1081 gccgaacatg gaaacaacaa cgcgcacacg gtccttatcg ggctcaagct cgtccaccgt
     1141 gccgatgaag ccctcaagcg ggccgtcggt gatcttgacg gagtcgccga gctcatagcc
     1201 gaccaccact tcatggtgct cgacgccgag gtcatagatt tcctgttctg tcagggggat
     1261 cgccttcccg tcggagccga cgaagcccgc ggcgccgcgc acgttgtgca cgagatgcca
     1321 gctctcatcg gtcagcacca tcttcacgag cacgtagccc ggaaagacct tgcgctcgac
     1381 ggtcttctca ccggaatcgg tgatctcctt gaccgtctcc atcgggatgt tgacttcctg
     1441 gatcagatcc tgcattctgc ggttttccgc agccttgagg atgtttgcgg ccacgctgtt
     1501 ttcgtagccg gagtaggtat gcacgacata ccattttgcg agttcagaca tattacccac
     1561 ccagcttcag gagaagctgc acgccctggt cggccagaga atcgaagccc cacagcacaa
     1621 gtgcggacgc gaacatgacc accaggcaaa ccgcggtgtt gtttgccgtc tgcttcggcg
     1681 tcggccacac gacttttttg agctcgctct tcatgtcgcg gaaccatttg ccgatacgct
     1741 tgaaaaacgg gagtttctta tcgactttct tgacggcttc ggtggaagtt tctttctttt
     1801 caggtttctt atcagccatg aatcattccg tcctttcgaa agccttattt ggtttcaacg
     1861 tgcttggtgt gcttgcggca gaaggggcaa tacttactca gttccagctt ttcggtagtg
     1921 ttcttcttgt tcttcatggt gttgtagttg cgctgcttgc actctgcgca tctgagcgtc
     1981 accttgacac gggatcctgc tttagccata ttgttcggca cctccttatt gatttcgccc
     2041 ctgtcggagc gtgattgcct ccctgtatga gccgaaaggg cataaaaagg gaacnnnnnn
     2101 nnnncacgag cacgacgccg atggcgaggc tgatgtagac gttctggatg aagcccgcca
     2161 gaatgtagtt gacaaagcag atgatgccga gcgttgcggc atacggcagc tgcgtggaca
     2221 cgtggttgac gtggttgcac attgcaccgg cagaggccat gatggtggtg tcggagatgg
     2281 gcgagcagtg gtcgccgaac acggctccgg ccaggcacgc ggacacgccg atgataaaca
     2341 gctcgctcgc cgggtcgaac acgtaggtga cgatcggcag caggatgccg aacgtgcccc
     2401 agctcgtgcc ggtggagaag ctcagcacac cggcgacaat gaagatgatc gccggcagga
     2461 agctgaacag agacccggcc gcggagttca tgacggaggc gacgaagtac ttgccgccga
     2521 gcagaccggt catgttcttg agcgaggtgg cgaaggtcag gatcgtgatg gcggggacca
     2581 tgttgatgaa gcccttcggc aggcactcca tggc
//
LOCUS       DAWBMW010000211         2612 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_7792_length_2612_cov_2.064529, whole genome shotgun sequence.
ACCESSION   DAWBMW010000211 DAWBMW010000000
VERSION     DAWBMW010000211.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7792_length_2612_cov_2.064529"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    852..941
                     /estimated_length=90
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          482 a          793 c          670 g          577 t
ORIGIN      
        1 cggtagccct gcttcttctc gaggttggcg aaagcgatga ccagcgggga accgttcttg
       61 aggttctcat agtgctggcc ggcggtgcgc tcgtggtagg cgatgtgcgt gtcgtccagc
      121 acgaacaggg acatcttcgg gccgagatcc agttcgccgt ccttggagac ggtggtgatc
      181 cagccgaggt tgttcttgat gaattccttg atttccggag taatgactgc catgtcgaat
      241 cctttcatga ttgatacgga taacgtatcc ttactctttc cagagtaccg gtttcgaacg
      301 cgactagccc ggatgttcgc tgtgaggaac ctcaccttcg cacagctttc atccggtttt
      361 tgcctgcttg gcgatgccat gccccggtcg cgcaccagcc acacacctgt cacacacccg
      421 acgcccatcg accggtcgat tttggccggt agttcccgta atctgtgagc cgagagagcc
      481 acaagttttc ctaactgtcc tgaggtgcac catgggtttg gtcgaacgtt tcagcaagct
      541 gcgcagccgc tggtcccgca gctttccccc gaatctcgcc cgactcatgt tgctgggctg
      601 cgctgcatta tggggtggca gctatctgct cgccaaattc gccatggagg cgattccgcc
      661 ccaatggctg atgggtctgc gcatgatcgg cgcctgcctg tgcatgctgg ccttgttcca
      721 taagcacatc gtgccgtatc tgacgccgaa aatcattgtg ccggcgctcg tggtgggcct
      781 gacctactgg ggcacgatgg tcacccagac catcggcctg cagaccattg aaccgggccg
      841 cagcgcgttc cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntctcgctga agccgggcgg
      961 ttcgctgtcg ctgagtgccg gcgactggct gaccatcgcc accgcggtca tcttctcgtt
     1021 caatctgacc tgccttggtg tctacaccaa acgcttcgac cctatcgccg tcacttttgt
     1081 gcagttcggc gttgccggcg tattgttcat catcggcgcg ttgttcaccg aacccatgcc
     1141 gaacgccagc tggctggcac cctcagtagt ggccagcgtg ctctatctgt tcctcggcgc
     1201 cacgatgagt gcccagatca tgcagaacat cggtctggct cacgtgcctg cctcgcaggc
     1261 gtcaatcatc atgtgtacgg aaagtctgtt cgctgtcacg ttctccgcgc tgttctgggg
     1321 cgagacaatc atgtggagtt cactggtggg ctttgcgctg atcttcgtcg ccgtgctgat
     1381 gtcggtaatc aagccgacca aacagtcgct gcaccggaac tgacgattca gtttcggcca
     1441 tggatggtca tgggttctcc attgccctga accgttacgg tgaaggccga ggctcttgaa
     1501 ggcgtgcaat cggaaatcgg acttggcatc gccaaactgc atttcgagtt gggtgccggc
     1561 aacggcagct tcgcttgggc tgcatgacgt tctgctgaac gtcgttttcg ctccaagcga
     1621 accaatttcg gttgttcttt gcgctacttt gcgccacttt gcgctgattc ccactgctca
     1681 tcggcaccat atttttgagt cattccacat tccgctggct aggttttgaa ggtctcgtgt
     1741 aggcatcctc tcggtatcac cgagccttaa gcaaccttcc agcccgacac tttacggtcg
     1801 tgtcttgtga agaagttttt cgaaggtctt tccctctcat ccatcgtggc gggcaccctt
     1861 gccgccgtca cctcattctt actggcatcg aaaatcggta tcgccggctc ggtaatcggc
     1921 gcggcagcca gctacatcgt ctccgcggtg gccaccaaaa tctatcaaaa catactcacc
     1981 gcctccggtg agaaactaca gtcggcctcg tcttccggca atgacgatga cactacggat
     2041 aataagtccg ataaggcagc cgaatccgag tccgatacga cgatgaccgg catggtcaat
     2101 accgtttcca ccgcgcgcaa gccgcgcgaa gtcgtgtcca atcgagcgaa cggacgaacc
     2161 tattcggtca atgaactgcg caaggcccgc tcccataatt cgaagcgcac cgccattatc
     2221 gtcacgctgg tatccggcct gctcgccgtg gcagtaaccg ccggcatcgt catgctggtc
     2281 acgcagggcc gtggcacgga ccatatcatc actccgtcca ccacgcccac gcaaaccgac
     2341 accaaaacgc agaccgacac gtccaatcag aataacggac tgccgcagcc gggcattacc
     2401 gacggttcga atgattccaa caccaaggac tccacatccg gctcgtccaa cgacggcacc
     2461 gattcaagca cgtccggctc aaccggcacc gactcctcaa ccgattccaa caccaatgga
     2521 tccaccgatg gttcgaccgg caccgattcc tccactaatt cgggcacgac tggcggttcc
     2581 accggttccg gcaccaccgg aggcaccacc gg
//
LOCUS       DAWBMW010000212         2579 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_7889_length_2579_cov_2.874010, whole genome shotgun sequence.
ACCESSION   DAWBMW010000212 DAWBMW010000000
VERSION     DAWBMW010000212.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7889_length_2579_cov_2.874010"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          527 a          726 c          758 g          568 t
ORIGIN      
        1 tgtgccgccg atgcgtaaca cacttgaact gaagcaaatg gccacctccc gtctcggcaa
       61 acaggtggtc gatttggtcg acgtgacgca gattttcgaa aaaccgcagg gcatggccga
      121 tatcgacccg gatgtggcgg cgctctccgc gtccgcttcc cgcgtggatg tggtcaacgc
      181 catgtacgcc gagccgcagc tgcatggttc cgttgaggtg gcggtcaccg acatgactga
      241 cccgcgcctg gtggacgccg gcgtgccgga agccgtcgag tccgctgagg aagcgcgtaa
      301 gaaggccgag gctgagagcg gcaatccgca gcttgctgac gatcgcgagg ttcgtcgcat
      361 gaacacgggt ggtgaaacca tcggcggtgg cactgctgca acggattcgg atgatgacac
      421 tgctgcagcc ggtgtcacct cggctgcacg caaggtgacg gtttccggcc gcgagattct
      481 tgacgatgtg acttggctga ttggcccggg cgaccgcttc ggcatcgtcg gtgcgaatgg
      541 tgccggcaag tccacgctgc ttaagttgat tgatggcaca ctcaccccga ccgcaggcca
      601 tgtgaacatt ggcaagacgg tgaagttcgc ggtgctctcg cagcgcctcg atgagctgga
      661 gaagctcggc aagtacaaga tcaaggaagt gctttcccgc tataagccga gctacattgt
      721 ggatggcaag gaagtgacgc cgggacagtt gatggagcgt ctcggcttcg aatccgccca
      781 gcttatgacg ccgatccgcg atctctccgg tggtcagaag cgccgcatgc aactgctgct
      841 gattctgctt gacgagccga acgtgctcat catggacgag cccggcaacg atctggacac
      901 cgatatgctc gccgtgatgg aagacctgct cgatacttgg ccgggcacgt tgattgtggt
      961 ctcccacgac cggtacctgc tggagcgcgt caccgatcag cagttcgcgc tgattggcgg
     1021 caaagtacgt cacttgcccg gcggcgtgca ggactacctc gatatggttg aggctattaa
     1081 gaacggcaag ggattgccgg ctgacacgcc tggcttcgcg ggcaccggta gcagctcggc
     1141 gaagtctggt aagggtaagg cggccgccgt caacgctgcc gcatctctcc ctcagtccgc
     1201 ttcgcggatg gctccctcgt cagagggagc caataacggt gaggactcca cctcgaagct
     1261 caccggcaag gccttccacg aggcatccaa gcgcgtgaat gccatcgaac gtaagctcgc
     1321 taagcttgaa gagcagaagg ccgatctcga ggcgcagatg gccgcccatg acccgagcga
     1381 ctacgagggt ctcaacaagc tcaatgaaca actcaccgcc gtcaatactg aaagcgatga
     1441 tctcgaagcc gaatggctcg aactctccga acaattggag tagcgggaga aaccgtaacg
     1501 ttcacaaggc ctcggcagtt tgccggggcc tcccgctatg caaactccca cggattaatc
     1561 aattccacgt cggtaccttc gaagtccttg atattgcgcg tggctatggc cgcatcccag
     1621 gaacaggcaa ttgccgcaat catggcgtct tgaatactgg taggtgtgcc cctgtgttcg
     1681 cgttcgacaa ggatatgggc ataatccgca gctgcctgag tgtcaaatgc gaacgttctg
     1741 tcctctaatg tcataagcgc cagcttgacg gtcgtatctg tgtactttcg tcgtttgcct
     1801 tcaggcatgc gacatattcc ggccaacaac tcggcgacgg tgatggcggt ggttgccaaa
     1861 ttgcttgtat cttggttcct cagccaattg gcaacgtgtt cgtctggctg tttcttgatg
     1921 atttcgctga tgacgttggt gtccagaata atcatggccg attcctattc aaaagatact
     1981 ggcggtcggg aatcggcgga atttcgctcg gatagtggga gttccccctt gtcgagtccc
     2041 tcaaaatcca tgttcttcat gaattcgcgg atctcgtcat agaaattggt ctttcgcgtg
     2101 atttcatgcg ccaccgtgcc agccagccat tcttcaagaa tggatcgcac ctgagattcc
     2161 atggagcggt tgtttttctc agccacttct ttgagctgct gaatctgctc atcgggtatt
     2221 tttcggatgg tcaatgttgc catgatgcac ctccttgttg ctgtcataat gctagcatga
     2281 ggtgctgtcg ttttgcttgc agtacgggtg atgaggtatg cacgcatgaa aaaaggcgga
     2341 tatgcatcat gcacatccgc cttttgagct tggactgggt cttcccgcag tcgccgaatc
     2401 agcgcccggt aacctcggaa ccgacgacct tttcgctcag ggcgcgcggg ccgcgggtca
     2461 cggctacggc accggagcgc accagctcaa tcacaccgta atgctccaaa agccccagca
     2521 gggcgtcgat cttgccttcg gcaccggtgg cctcaatggt cagcgattcc ggattcacg
//
LOCUS       DAWBMW010000213         2575 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_7908_length_2575_cov_2.683333, whole genome shotgun sequence.
ACCESSION   DAWBMW010000213 DAWBMW010000000
VERSION     DAWBMW010000213.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7908_length_2575_cov_2.683333"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          449 a          792 c          803 g          531 t
ORIGIN      
        1 aaggcggatg ccgtggccta catgcgctac ctgtccgtgg cgagccggcg cggctatacg
       61 caccggcgca tcacgccgga cacactcgcc cggcttgaag acggcaccgc cgtgattgcc
      121 ggctggctca acggcgacaa cgcttccggt ccggccaaca ccgcgctcga caaagttcag
      181 ctgctagtgc tgttcgccgc gctgatcggc gtggaaccgg cgggggaggc cgcccgcgag
      241 gcgtggggag acacgactct gacggccttg gccccgttca ttcagaaggt cgcggtcccc
      301 tcccccacgc gcgcgctgga atcctgggac aagcagctgc tcaagggcct gaggagtcgt
      361 atcaccgcgc tggctgacga ggagacggtg gaatccgccg aaccggtgac gctggcccga
      421 ttctcctggc gttcgatgat caccatgctg ctggtcatcg tggccgtggt cgtggtcttc
      481 acccaactga agcccgagga gatcatcacc gcgctgacca acgccaaccc gttgatggcg
      541 gtggtgacgc tcgcgttcgg tgtctgcggc tggatcggct cgtcgatttc gctcggttcc
      601 ctgatggcgc ggcacaagcg tgacaatatg ggcgtcttca tgagccaggt ggcaggcggc
      661 ttcgccaccg tatccatgcc tgccggcgtg ggcccctcgt tcgtcaacct gcagttcctg
      721 cgcaaatccg gctatcgcaa caccccggcc accgcaatca tgagcgccgc gctcgtggtg
      781 tattacgccg tgtacttctc catgctggtc atcatcggcc tgttcaccgg ccgcaacatg
      841 ttctccggcg caatcccgac aaacacgttg gttatcgtgc tgggcgtggt ggttgtggtg
      901 ctgtccattg cgatgatgat tccgccgttg cgccactggg tgacccgtcg tcttatgccg
      961 ctggccaaga cgtatatcaa ccagctgctg gacgtgcttt cccagccccg acagctcaca
     1021 gtcagttgcc tgggcgcgct gttccagaac gcgaccactg ggctcgcttt ctgggcggcc
     1081 ctgcaagcgt tcggccattc atcgaatccg attgaaacga cgttcgtctt cctgctggcc
     1141 tatgcattgg gttccgcagt gcccactcca ggcggtctgg gcggtgtgga agcggcgctg
     1201 acattcgcgt tcgtggcggt cggagtgccg cagggcgtgg cgctttccgc cactttgctg
     1261 caccgcgtgg tgttctactg gctgcgtatt ccgctgggcg cggcggccat gaagtggctt
     1321 gacaagcata atcttgtctg attcgtctat tttcataccc ccttcgggga aatagatgtg
     1381 aaaaccctta taaaacgcgg gttttcgcag aaacatgcgc tagtatcatt gatgacaaca
     1441 tggactaagc aaaagtgctt gtcccctgac ccaagaagga tgctttatgg catacaacaa
     1501 gtctgacctc gtttcgaaga tcgcccagaa gtccaacctg accaaggctc aggccgaggc
     1561 tgctgttaac gccttccagg atgtgttcgt cgaggccatg aagtccggcg aaggcctgaa
     1621 gctcaccggc ctgttctccg ctgagcgcgt caagcgcgcc gctcgcaccg gccgcaaccc
     1681 gcgcactggc gagcagattg acattccggc ttcctacggc gttcgtatct ccgctggctc
     1741 cctgctgaag aaggccgtca ccgagtgacc ttctgctcgt agcgattact tcgagcatta
     1801 ctgacgacaa agaccccgac cgagatggtc ggggtctttt tgttgtggtg ctgtgacgtg
     1861 ttgtccaacc gtattattcc ggactagttc agcgcttcca taagctgctc gaatcccagg
     1921 gtgggctcgg cctcgaaggg atcgaggagt atggcttccc ggcgttcggg ggcggtcagg
     1981 gccaggtggg tccagtcggc ggagaactgg gctccgacgt tcagggccgc gtccacgaac
     2041 cggccacgca gggcggcacg ggtgtcggtc ggcggattgt gcacggcgta ttccacgttg
     2101 tctcctgtca gcagctcacg catctggttg cgggctacga gcgaaccgta caggcgaccg
     2161 ttggcgatgt cgtggtagtc gagctcaagc tgttccatct gggcgaaggt gacgtccggg
     2221 cgacggcgtt tgagcgcgtc gaacagacgc ttcttggcgg cccagtccac gcggtcggcg
     2281 agcgcatcgt atgcaccacg ctccagcgcg tccagaacgc ggctccactc ccccataatc
     2341 gtgtcgatcg tggtggccgg caacgaactt gccagcgaat cgacatgggt ttcgatgaag
     2401 gccttgaccg ccgcataata ccggcgctgc agacccagtg cggacacgga ttcgcctgat
     2461 tcgagcgtca gttcggcgcg cggattgtca aggaatcgac tgaccgtgcg attggcggca
     2521 gccggatcag caaaggcata ctgctcgaat ccggacggaa cgccatggcg gaagg
//
LOCUS       DAWBMW010000214         2568 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_7928_length_2568_cov_2.926383, whole genome shotgun sequence.
ACCESSION   DAWBMW010000214 DAWBMW010000000
VERSION     DAWBMW010000214.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7928_length_2568_cov_2.926383"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          576 a          778 c          737 g          477 t
ORIGIN      
        1 ggtcgcaaag gtattgctca ccttgattct ccttctaatt actcgaacat tgttcagact
       61 accgccttga agcgactgaa ggcggctttt cgcgtgcttt ttggcgggca gcgtagggat
      121 ttcggtttat tcgagggtgt cgaggacgtc gtcaatgagg aggcgggtct gggaggcggc
      181 atcgtcgccc agacgcggga agcgacgggc ggcattggcg gggctggcac cggcggctcg
      241 cgctacgttg gacttgctgg caccatgggc cacggctttt tccgcgagcc gatcgaccag
      301 tgaatcggcg acttggcgca tctgttcggc cacccataat tgggccatat ccgcaggtaa
      361 gtcgtagtct tcggccacat cgctggctgc ctcctgaatg gcggcccata acaccatgaa
      421 tggctttcga tagtagtcaa tctgctcagt gagcggctca gcggcaagca attcaagtgc
      481 cgagcgaatt tctgtatttt ctgacatagc tttctttacc tcggctcccc tctctgaggg
      541 gagctgtcgc cataggcggc tgaggggagt ttcgaccggg gcagctcccc tcagagaggg
      601 gagccagaat atatgtattt acttacgact cagcaaccac tttgatatca gtgacgacgt
      661 ggccacggcc atgccgaccg gcacaaagaa ggtaatcggc gcttcagaga gctccatcac
      721 caagcacatg gccataagcg gtgcctgttg tgaggccgaa agcaacgaag ccgcaccgat
      781 caaggcacac gcggttaccg aatcggtcgg aaacatcagt atccatacga ggccgagcat
      841 cgcacccagc gtggaaccta atgcgatgcc gggctgcagc acaccgcccg acgcaccgga
      901 gcgaatggtc atcagcgtca ccagcgcttt ggcaacaaag gtgagtgcca acacacccag
      961 caacatccag atattggact gggacagcgg gaaaccggcg ttcatcacgg atccagaagg
     1021 gccgctccca ccctgaagca tggcccagaa caatgccatt ccagagcccg ctcccccgcc
     1081 agaggccgaa tcggatacat taccgccgcc cgtcagcaag ttccatggag aggcggccgc
     1141 tgaagatgcc gactgcatgg caccagcagt gccggcggtt tcaggaatga atgtgctgaa
     1201 tccaagttgc gctgcggcac ggccgttgcc catcacctgc ggcacaacag tcgccaccaa
     1261 accggtgatg agaccagcca gcggcatctg ccacagaata gccttaccgg ttggcttgtg
     1321 cgcttccgcc cattgcgaac cttttcggaa caatgcgccg cccactccac atgccgcacc
     1381 gcagatcaga gcgaacacca ttaacgttgg cgtggattcc ggttgcatgg cggtaatgtc
     1441 gtagaacagg tgatgccctt tgatcgcaac cgccacgaat gcggcaatgg cagacatgcc
     1501 cagtccaaac gcaacttttt cgattgtcac atctaccaac aggatttcga cggcgaagaa
     1561 catgccggcc agtggcgcgt catagacgcc tcctagacca gcaccggcag ccactgccac
     1621 caccatgcgc cggtcgatgc cgtcaacgcc ctcgatatgg aacaggtcgc agaaacgttg
     1681 cgcgagcatg gcgccgagtt cgcgtggggc gacttctcgg ccgatcgagg caccggagcc
     1741 gacgatgaaa atctgcaata ccacatgcgc aatggtttgc cagaaaggca ttcgctttcc
     1801 cgccacggcc ttcttgactg agggcaccgc agtcgttttg tgtcgcatca gataccagat
     1861 aatgcctgcg atgctgagtc ccacggtcac cgatatcgca cggcgtgcag cgggcacgcc
     1921 gaatggacca ggcaattctg ggccttcgat gtagccgagc agcacatgtt caacgccgta
     1981 gagcaacagg gtcagcagac ctgctcccac accgatgagc acgccgagca caatggtcgc
     2041 cgcagcgagc caaccaaggc gtttgatagt aaccgttggc tgcatgtcaa cctccgcacc
     2101 ctatgcaacg agtatgcctt gggaactccc ctcagtctcg ccacgctcga cagctcccct
     2161 caaagagggg agccaaagaa atagtccttt tgccagaata ggaagagccc ggaaccatga
     2221 aacacgattc cgggctcaaa caatcacgct aacgcgatga ggctatgctc actcagcatc
     2281 cttggcgtca tccttggcgg acagttcgtc ggccacggcg gcagcagcgg aagcggcctc
     2341 gacggactcg gactcttcgt cctcagcggc ctcgcccagg aaggcggaga ggtccacaac
     2401 ctcgccatca gcggtgaact tgacggcgcg catgccggcg agcaggccct tggaacggcc
     2461 gacttcctgc acagcggagc caagctggcc attcttgatg atggcctgga tgaagttgtt
     2521 cggatccatg ccgtactgct gggcgatgga agcgaggaag ttgaacac
//
LOCUS       DAWBMW010000215         2548 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_7999_length_2548_cov_2.391496, whole genome shotgun sequence.
ACCESSION   DAWBMW010000215 DAWBMW010000000
VERSION     DAWBMW010000215.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7999_length_2548_cov_2.391496"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1859..1868
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          560 a          765 c          728 g          485 t
ORIGIN      
        1 gcaactggac attgacaccg agacgagcgg cggcagacgc ctgattcgcg cccttgccgc
       61 ccggcagcac tttcatggca ccaccattga cggtttcacc tggcttgggt agtcgtttag
      121 tggttacggt gtaatcggcg ttcatcgagc cgaccacgat taccgagccc ggcgcatcct
      181 tcagcgcttc caatcgttcc cgtacgggct cgggcatctg ttcatgttct tcttcactta
      241 tcaccatggg ctccaagcat agtaggcgtc gggcccaaat gcgcatatga tcagccgcgc
      301 aacttgcgaa gacgctcgtg catggccacc atcagcacca agagcaccag caaataccac
      361 gggtagagac tgattgtcgc atattgcgcg atgatgccga acagtggcgg catgagcagt
      421 gagcccacat aggcgcaggc catctgcacg ccgacgatgg cctgcgattt gtcttcgccg
      481 aagtaggccg gcgtcgagtg gatgacgcac ggataaatcg gcgcgcaccc gaagccgatg
      541 accaccagcc caacgaccac gccaaggtgg tgaggcaatg gcacgaacat ggtgaggatg
      601 ccggcgaaca ccagcacttg gccgaggcga atcatgaccg ggtccttgaa tcgcatggtc
      661 aggaagccgg aaagcgcacg gcccacggtg atgcctacgt agaataggct ggcccagctg
      721 gcggcggtga tcttgtcgat gccgctgtgc atcaccatat agctggaggc ccacaggccc
      781 gccgtagatt cgacggcgca gtagcagaag aacatcacga ggatttcctt ggcaccgcga
      841 atggcgagca cgccggccac gccgagcggt ttgcgttcgg caacagacac cccttcagca
      901 gtgccaaatc tctcggcatt gccgtcagat gatgcggtgt catcggtcga ttcgccgacc
      961 gcaattgcgc cacgtttctt ccacagcggc aggctcagca ccaggatgac ggtcagcatc
     1021 acctgcagga tcgcgatgta ccgatagccc cacggccaac cttggccttg agagagcgca
     1081 tagcccatga tgtaggggcc gaccgatgcg ccgacgcccc acatgcagtg cagccagctc
     1141 atatgccggc tttcgtagtg aatggctacg tagttattga gcgccgcatc cactccgccc
     1201 gcgccaaggc cgtatggtat ggcgatggcc aacagcaccc agtaattcga ggtaacggag
     1261 aaaccggcca aggccgccgc agtcaacgcc accgaaacgg cggtgacctt gcctgccccg
     1321 aatttcaatg tcattcggtc cgaaagcagc gccgagacga tcgtgaacat ggaaatcacc
     1381 gcggagatgc cgccggccca cgccaccggc acgttcagat cctggctcat ggtgggccat
     1441 gcggatccga gtagcgaatc cggcaatccc agactgataa acgccacata gatgacggca
     1501 agcaacagac tggccatgtc ccatctccca ttcacagtac ggcaccggtt gtgataacga
     1561 tgtcatcagt ctatcgaata gcgcttcagc aacacgcaaa catcacagtt tggagtcttg
     1621 cccgcagtac accggcgtgg aatcggctac gccgcgcggg tcaaagccga tgatgtcgta
     1681 gttgtccttg agataatcgg cggcggtgca ctcgatgaaa tcctgatgat ataccgcagc
     1741 accctctgac gaaatagctg tccgccaaag acggactgag gggagagaaa tacgagtagc
     1801 ccgctaccac ccgaggcctc tcccccagtc agcttcgctg acaccccctc atcagaggnn
     1861 nnnnnnnngg ttgtgataac gatgtcatca gtctatcgaa tgactcatgg gcaacacgca
     1921 taaaacatat gacaacgtgc tcacctcaaa cgccaattac ggaaattctc ggtaagcacg
     1981 atgtcattat cagcgacgtc ttcgatcagg tatcgatctt ggagcggtcg aaatcgtcgg
     2041 cgttgtcgac gatgaactgc ttgcgcggcg gcacgtcgtc gcccatcagc aggctgaaga
     2101 tctcggatgc ctgggcggcg tcctccatgc ggatgcggcg cagcatacgc gtgcgcgggt
     2161 ccatggtggt gtccgccaac tggtcggcgt ccatctcgcc cagacccttg tatcgctgga
     2221 tgtcgtcgtt gtatccgatg cgcttcttgt cgaggtcggc cagtttgccg gccagctcgt
     2281 catcggaata cgtgtagata tattcgccct tgtgcgagcc tgtcaacgcg atgcggtgca
     2341 gcggcggcac ggcggcatac acgtagccgt gttcgataag cggccgcatg taccggtaga
     2401 acagcgtcag cagcaggata cggatatgag caccgtccac atcagcatcg gtcatcatga
     2461 tgatcttgtg atagcgcgac tgttcgatgt cgaagctctg gccggagccg gcacccacca
     2521 cttggatgat gtccgcgcac tccttgtt
//
LOCUS       DAWBMW010000216         2529 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_8072_length_2529_cov_2.227567, whole genome shotgun sequence.
ACCESSION   DAWBMW010000216 DAWBMW010000000
VERSION     DAWBMW010000216.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8072_length_2529_cov_2.227567"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    294..303
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          461 a          688 c          818 g          552 t
ORIGIN      
        1 gtcgttcgga atggtgacgg tgtcgccctg cttcaggtcc ttgatgtcct tgactttggt
       61 ggagtacacg ccgagcgggt agatggcggt gccgccgatg ggctgcaggt ccttcttgtt
      121 gctcacgttg tagttggcca agtagagcag gtgctggaat tcgttgaggt ccagctcgcc
      181 ggagtcaagg gccgggtttt cggaggtgta gtcctggaag tcgacgatgt cgacgtagat
      241 gccggccttc tcggcgtcct gcttgaactt gacccattcg gggttggtag cgcnnnnnnn
      301 nnntggtagc gccgaccacg ccgatcttga ccgggttgcc cttgctgccc ttggcggacg
      361 actcgttggc accggcaaac gcgcggtagc cgaagaacgc ggccacggcg ataatcgcca
      421 cgaccacgat ggtgatgatg acgttgcggg cggtgtggtt cacacgcacc ggctcgttat
      481 tgttaggagc ggccggggtt gccggctggg ttgctgctgt cattggattt cctctctcca
      541 aaattgatgt cgggtaacac cataacgcgc ttgaaccgcg aaagtgacgg aaaatggccg
      601 attgccatat aggtgtcttt tatggcgcgc atgcaatgtc cgggattcgt gcgtttgata
      661 acccgctata ggcgtgccga acacgccgga aagcacgtcg agaggggctt tgacaatgga
      721 agctatgact accgatactg ccaatgccga tatccgagcc gtattcttcg atatcgatgg
      781 cacgctcacc agtttcacga cccatacggt gccggattcc acaatcgagg cgattcaccg
      841 gctgcaggcc gcaggcgtca aggtgctgat ttgcacgggc cgcgcgcctt cacagatgca
      901 cgtggtgctg gataccatgc cggtgacgtt cgatggcgtg gtggcgttca atgggcagta
      961 ttgttttgat gacaccggct tctttgcctc gcaggccttg gatactgctg atattgaggt
     1021 gattctcgac tggctggacc gtcaccccga tgtggtgtgc aatttcggcg agaaggatta
     1081 cgtgtacttc aaccagtcca atgatctgct tcgcgccacg tggagcaaac tgggcaaaac
     1141 cgcaccgacc cggtatttcg aggacccgcg cacccgcgcg ctggatcatg agactttcca
     1201 gatcagtccc tttgtcgggc ccgagctgga ggccgaactg gtgggactgt gcagcaatgt
     1261 gcgcggggtg cgttggcatc cggatttcac ggatctgatt ccggccgatg gcggcaagcc
     1321 gcgcggcatc cagcggttca tggcgcatta cggcatcacg cgcgagcaga ccatggcctt
     1381 cggcgatggc ggcaatgaca cggatatgct cgcctacgcc ggcatcgggg tcgcgatggg
     1441 caatgcgacc gccgagccca aggccgccgc cgactacatc actgacgatg tggaccacga
     1501 cggtgtgcgc aatgccctgc tgcatttcag ggtgctacgc gattaaaaca tcacaacagt
     1561 tcgctttgtt ctgccccgcc gtgacagggc ttatgaataa gcaaagggct cgggacgaat
     1621 cccgagccct ttggtgttag cgaactaaca acaccgtgga ggtgttatca ctcgttgtcg
     1681 ccagcggtag cggcggtagc ggtgacggca cccttcttgt cggtgttcac gccggagtac
     1741 acccagtcgg tgtagtccgg gtggtcgtag ccgttgtcga cggcgaactg gaaggcctca
     1801 tcacggaact tctcgagctc gtcgatcttg tcggcgtact tgtcggcgtc gatcatgcgc
     1861 agagcctcag cggtcagctc gtagcggtcg atgcggttga cacgaaccat gtcgtacggg
     1921 gtggtggtgg agccctgctc ctcgtagccg tggacgttga agttgtcgtg gttcggacgg
     1981 tcgtagatca ggccacgcac gtcgtgagcg taggagtggt acgcgaacag caccggcttg
     2041 tcggcggtga agatgtcggc gaactcctcg tcggtcagag cctcgtcgtt ctccttggcg
     2101 gactgcaggg agagcaggtc ggcaacgttc acaaccttga acttgacgcc cagttccttc
     2161 agcttgtcgg aagcagccat gatctcctga gtcgggacat cgccggcggc ggcaagcacg
     2221 acctcggcct catcgttgtt cttggcggtg gaagcccaat cccaagcggc ggcacccttc
     2281 tcgagctcgg cacgagcctc gtccagggtc agccaggtgg cagcaggctg cttaccagcg
     2341 atgatggcgt tgatcttgtt ggtggacttg tagcacttct cggcgatggc cagcagcatg
     2401 ttcgcatcgg tggcgaagta gatgccgatg acgtggtcgt tgtggaagca cttgttcagc
     2461 aggacggagg tgacacccgg atcctggtgg gagaagccgt tgtggtcctg acgccaaaca
     2521 tgggaggag
//
LOCUS       DAWBMW010000217         2514 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_8126_length_2514_cov_2.552664, whole genome shotgun sequence.
ACCESSION   DAWBMW010000217 DAWBMW010000000
VERSION     DAWBMW010000217.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8126_length_2514_cov_2.552664"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          538 a          830 c          750 g          396 t
ORIGIN      
        1 aattcccctt ccacgtgatc atcaacccga gctacaaacc caccagcgac aaaaccgcct
       61 cgttctacga gggctgcctg agcttcgacg gataccaagc tgtgcgcaag cgttggctcg
      121 acatcaccgc cgaatgggat gatgaagacg gcaaacacca cagcgagccg ctgcacggct
      181 ggcccgcacg catcttccag cacgaaaccg atcatttgag cggcgagttg tatatcgatc
      241 gcgccgaaat ccgttcgctg accaccaacg agaacctcga ggactactgg tgcgaagacc
      301 cggtgcccac cgaggccgct gaagagctcg gtttcgcact atgaccgacc gtgcatggtt
      361 cgacgccgcc aagggagaca ttccccaatg ggcggcgtcc atgcccaagg acgccgatgg
      421 caccaccatc gactgggact gggtgcgttc tctggacctg gcgcatggcg tcggcacaat
      481 accggaatcc accccacagt ccatccacga cgcattgaag gccgactacg ccacacgcga
      541 gcgtctggaa tcgatcatcg cccaatgcaa cagcgcattc atgaccgatt tcgaccggtt
      601 catcggctcc tatcgtctgc ctcgcgcgct cgcctacgcc aatcgtcggc tcaacgacaa
      661 catcaacacg ctgttgcatc tgggcgaaca gcaacaccta tgggcggtca gtcgcgaacg
      721 gcgggggagg gccaccattg ccgtgctcaa gccgtactcc ggcagcggcc cgtcgaacga
      781 tgaccaaggc atgctggatc tgagcggcga agccggtggc acatctgccg gaaacactgc
      841 cgccgaaaac agcgatgtca agttccccac aacagtcgcc ttggacgatg tccaagatcg
      901 tacggcagcg attcttgacg cacgcgccga acggcgtaag gcccagcgct tccagaagcg
      961 ggcgggcgat gcggcgatgg cccgcgccaa tctctccggc gcgacttcgc cgggtggtgc
     1021 cgctctgccg ggtggtgccg ccgcgtctgg caacgctacc ggacaccatc ccaagcccga
     1081 ctggcgcgac gcctacctgc ccggccgcga cgtggatgcg gtgatgggca tcgacatcga
     1141 aaccaccggc atcgatccgg cacgtgacta catcatcgac gtcggcttcg agttcatgaa
     1201 catggccagt ccgcgcccgg ccggcgaacc cgagacatac gcctatgagc agggctatta
     1261 cgccgccggc gacgcctacg gccagtcccg cctcgctttt ggcgtgccgc cggaaaacgc
     1321 ggcgctcggc aacgccctca tcgccaaact caccggcatc gatgtacgcg gccgcagcag
     1381 cgaggccggg taccgactct tcgacgaatg gccccaagcc caggccggcc tgctcgcgcg
     1441 gcttacccag cagccgtacg tggcgcataa cgccaccttc gaacatagct ggttcatgct
     1501 gaacgtcgcc ggatacgcgg aaagctaccg cgccgggcgc atcaccatca tcgataccct
     1561 gccgatgagc cggcagtggg accccggcgc ggtacccacc aacgagcatc cttacggaga
     1621 caacacgctc gacgcctacg ccaaacgtca gggcgcgctt gattccgccc acaacgaacg
     1681 ccacctgggc cttgaggatt cgcacatcat gctggtggcc atgaaacacc atctggcggc
     1741 gctgaaagcg caagggaaag ggccgtgggg gtccaccggc agggcgggcg tcggcggcaa
     1801 gtcctgcggt cgtaagcggt gagtacacta ttggtctcgt gagtaactat caggacagcc
     1861 gacagcagcg tgagaaccgc cgccgcacca tcgtgcgtgt ggtgtgcgtg gtcatagctg
     1921 tggccatgct cggctccctg gtcatcccgg cgatttacgc ggggctttga cgttgccttc
     1981 gcatattcgt gcccgcagtt gaacacgtct gatgcgaggg attcgtgagg tcgcccggcc
     2041 ggttatcggc aaggcaggcc cggaaccttc tgacagcatg gattgcaggc ggatgcgcgt
     2101 gtggcgcggc aatgcccgcc aacgccagag gaatgtgcga aggctgtcaa caaggacgtg
     2161 acttcccaag gaatctcaaa gaatcaacag taaggtaaac cacgatggca gaatttgact
     2221 ttacccaggc gatcggcgaa gcccgcgcca aatacgaatc catcgccaag gcgctggacg
     2281 tcgaccgact caaggccgat atcaaggatc tcgaaacgca ggccgcggcc ccgggcctgt
     2341 gggatgaccc ggaaaacgcc cagaaaatca ccagccggct gtccgccgcg gaatcgcagc
     2401 tgaagcgtct gaactccgcc tcccagcgca tcgacgatgt ggaaacgctc gtcgaactcg
     2461 gccacgagga gggtgatcag gactctctgg acgaggcgca aagcgaaatc ggcg
//
LOCUS       DAWBMW010000218         2471 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_8280_length_2471_cov_2.617136, whole genome shotgun sequence.
ACCESSION   DAWBMW010000218 DAWBMW010000000
VERSION     DAWBMW010000218.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8280_length_2471_cov_2.617136"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    227..236
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          420 a          714 c          795 g          532 t
ORIGIN      
        1 catgtcgtcg acgtcgctga tcttcttgat caggggcttg ggatcgatgc catgctcctg
       61 gttgtaggca atctgcttgg cgcggcgacg gtcagtctcg tcgatggctt gccgcatggc
      121 ttcggtggtc tcgtcggcgt acatgatcac cgtgccggac acgtttcggg ccgcacggcc
      181 gatggtctgg atgagcgaac gataggagcg caggaatcct tccttgnnnn nnnnnngcga
      241 tacttccggc aaatcgaggc cctcacgcag caggttgata ccgacaatca catcgatctt
      301 gccttcgcgc aacatgcgca gcagttcgac gcggcgcaac gtatccacat cagaatgcag
      361 gtattcgacc ttgatgccgc gttccagcag atagtcggtc aagtcctcgg ccatcttctt
      421 ggtcagagtg gtgaccagcg cgcgctcgtt cttggccaca cgggccttga tctcggcgag
      481 cagatcgtcg atctgcccct tgaccgggcg tacgtcgatc ttcgggtcca gcagaccggt
      541 tgggcggata atctgttcga ccaccccatc ggacagtccc atctcatagt cgcctggcgt
      601 ggcggacagg tacacggtct ggccgacgcg ctgcaggaat tccggccatt tgagtggtcg
      661 gttgtccatc gcggagggca ggcggaagcc gtgttcgacc agcgtgcgct tgcgggaggc
      721 ataatgggag gccgggaaga tgtggacttc gttttcctcg tcgatctcgt cgccggtcag
      781 cgggtgcagc gtggagatgc ggtcaatctc gtcgccgaag aattcgatgc gcacggccag
      841 ttcctcgtag acggggatga tttcgaccgt atcgccgcgc acgcggaagg tgccgcgcgt
      901 gaaggcgatg tcgttgcgct tgtactgcat ggccacgaat tggcgcagca ggtcgtcgcg
      961 gttgatttcc tgcccgactt tgaggaagag catgcgaccg gcgtattctt ccggcgtgcc
     1021 caagccgtag atgcaggaga cggtggccac aaccacgcag tcgcggcggg tcagcaggtt
     1081 tgcggtggct tggtggcgca ggcgctccac atcgtcgttg atgttggagt ccttttctat
     1141 ataggtgtcg gtctgcggga tgtatgcctc gggctggtag tagtcgtagt agctcacgaa
     1201 gtagctcacg gcgttgtccg gcatgagttc gcggaattcg gcgcatagct gcgcagcgag
     1261 cgtcttgttg ggctcgataa tcagggtcgg tcgctgcagc ttttcgatca gccaggcggt
     1321 ggttgcggtt ttgccggtac cggtggcacc cataagaacc acatcattct cgccgttttc
     1381 gatgcgttcg gccagttcgg cgatggcctg cggctggtcg ccggatggct tatatggcga
     1441 tttgacgacg aaaggcttgt ccgcgcgctc gatattgaat cccatagggc ctcatcttag
     1501 cgacctgcgc accatcctgc gtatatctca cccacttggg ccagcatctc gggcaatgga
     1561 tgggtggagt cgatgacgat atcggctatg gcccgccgct cctcctcact ggattggtgg
     1621 cggatgcggg cctcggcctg ttccaggctc atgccgcgtt cctcgaccat acggtcgagc
     1681 cgcatgcaca cgggagcttc gaccgtgacg atatggtcaa aggcgaatgg aatatcgtca
     1741 atgacctcgg cgagcagtgg aatgtcgtga atgatgatgg ctgcctcggg gtgctcatgc
     1801 tccagtcgcg ccgcctcggc atagatcaat gggtgctcga tggcgtcgag ccgttcacgt
     1861 gcgcccggcg cggcgttcgc gccgaagaca tgatcggcaa tccatggccg gttcatagtg
     1921 ccatcggcga gcaatgcatc aggaccgaac tcggcgacga tctgtggcag cgccactccc
     1981 ccgggttcga cgatctgatg cgccagcgca tcgtagtcga tatgcaacgc gccgagttgc
     2041 ttgagttgcg ccgccaccgt acttttgccc gcggcgatgc cgccggtcag tcctattcgc
     2101 atcatggtgc ccatgatagc tggcgctccc ctcggccggc tatcgccgac agctcccctc
     2161 agggagggga gccggctcgc gtagcgagac tgaggggagt cattggcgga taactccaaa
     2221 aacacaagaa acccggccca atcggaccgg gtttctttac acgctaagcg ctagcttcgc
     2281 ggcaaactca cttgccgagc agctggtcgc gcagggcggc gagctgatcg gagtcggcga
     2341 gggtaccggt agccggagca gcggaagagt agttggaggt ctcctcgacc ggcttctctt
     2401 ccttcgggcc ctggccgtcg gcggctgcgg attcggcagc gttctccagc tccttggcca
     2461 cgaactcctt g
//
LOCUS       DAWBMW010000219         2439 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_8398_length_2439_cov_2.104446, whole genome shotgun sequence.
ACCESSION   DAWBMW010000219 DAWBMW010000000
VERSION     DAWBMW010000219.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8398_length_2439_cov_2.104446"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          525 a          819 c          689 g          406 t
ORIGIN      
        1 gccgatggaa tacgcgcttg aactcaacag gcttgtggaa cttcagatgg aaggatcggt
       61 gggctgattc cgatgaccga caacacaaca cccgtcaagg aaatcaagat tccggtcgcc
      121 gaccccaacg acccgtacgc gattccggcc gctatgccgt cctccgtgga ccatgccgtg
      181 cgctcgttca acgcaggcga ctacccggag cccagccgca agcaggacga atggcgctac
      241 acgcctatcg agcgcatcaa cgagttcttc accgtgttca agccttccgg cgaaacgcag
      301 atcgaagtct ccatgattga cggctcgccg ttggccgaag gcgtgaagct cagccagatc
      361 aagctcggtg agggcctgtc cggcactgtg tccaagccca acgaccgcgt ctccgcagtc
      421 gaatgggaat ccggccgcac cgcaaccatt ctggaactgt ccggtgagat cgcccagccc
      481 gtgctcgtca aggtgcacgg cgcgggggag gacctcgacg ctttccatct ggtgatcgcc
      541 gccgccgaca aggctcatgc ggacgtcgtc gtggaacacg acggtgacgc acgactggcc
      601 gaaggcgtgg aaatcaccac cggcaaggac tcgcacgtct ccaccacgtt cgttcaggaa
      661 tgggccaaaa ccgccaagca tgtggccaac caccgcatcc acgtcggcga aggcgcatcc
      721 ctgcgccact ccgtcgtgac cttgggcggc gacatcgtcc gcatccgcat ggatcaggac
      781 ttcggcggcg aacagggcga cctcaacatg ctcggcatct acttcgtgga ccccggcgag
      841 cacatcgaac accgcacgat ggtggtacac aaccaccccg actgcaagtc ccgcgtggtc
      901 tacaagggcg cgcttgacgg caagggcgcg cactccactt gggtgggcaa cgcgctcatc
      961 cagcccaccg cccccggcac cgactcctac gaccgcaacc tggtcctgac cccgggcgcc
     1021 atcgccgatt ccgagccgaa cctcgaaatc gaaaacggca acatcatcgg tgccggtcac
     1081 gcctcttcgg tgggccgctt cgacgacgag gaactgttct acctcgaatc ccgcggcatc
     1141 cccgaaaccg acgcccgcaa gctcgtggtg cgcggcttct tcggcgaact cgtcgaggag
     1201 atcggcattc ccgccatctc cgaacatctg atgaccgtga ttgaccgccg actggcccgt
     1261 ggtgaaaacg acgccatggc acaggtcctc gaagacaagt aagaacagca tttttaggag
     1321 cacaacaatg tcaacactcg aaatcaagga cctgcacgtc cacgtcgaaa ccaaggaagg
     1381 catcaagccg atcctaaagg gtgccaccct gaccgtccac tccggcgaaa cccacgccat
     1441 catgggcccc aacggttccg gcaagtccac tctcgcctac acgttggccg gccaccccaa
     1501 gtacgtggtc gactccggcg aggccctcct cgacggcaag gacatcctca agatgactcc
     1561 ggacgagcgc gccaaggccg gcctgttcct ggccatgcag tacccggtcg aaataccggg
     1621 cgtgtccatg accaacttct tgcgcaccgc caagaccgaa atcgacggcg aggctcccgg
     1681 tattcgccag tgggccaagg acctgtccgc agccatgaag cgcctcaaga tggacccgaa
     1741 gttcgcctcc cgttccgtga acgaaggctt ctccggcggt gaaaagaagc gcgccgaagt
     1801 gctgcagctc gaactgctga aaccgaagtt tgccgtcctt gacgaaaccg actccggctt
     1861 ggacgtggac gccctgcgca tcgtgtccga aggcgtgaac cgcgccaagg aagccaacca
     1921 gttcggcatc ctcatggtca cgcactacac ccgtatcttg aagtacatca agccggacat
     1981 cgtgcacgtg ttcgccgacg gtcacttcgt caagaccggc ggccctgagc tggccgacga
     2041 actcgaagag aacggctacg accagtacct gcccgaaggc gccgactccg agtccgcact
     2101 ggcctgagtc cgtgaccgtg ggagtctgac atgccctatt ctcgacacgc tccgggccgc
     2161 ttaggccagg tctttacggt cgcgaacagg ccatgtcaga ctcccactcg tttcgtacaa
     2221 ttgttacgta agtaggggga acataatggt tgattttaag gcgattcggg cgcagttccc
     2281 gattctggat caggagattc acgggcaccc gctcgtgtac ctggattctg cggccacctc
     2341 gcagaagccg aatgcggtga tcgacgccga ggcgaacttc taccgtacaa ttaacgccgg
     2401 cgtgcaccgc ggtgcccacg agctggcggc ccgtagtac
//
LOCUS       DAWBMW010000220         2425 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_8447_length_2425_cov_2.494515, whole genome shotgun sequence.
ACCESSION   DAWBMW010000220 DAWBMW010000000
VERSION     DAWBMW010000220.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8447_length_2425_cov_2.494515"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          468 a          695 c          776 g          486 t
ORIGIN      
        1 cggctcggct catcagaggg aggttgtgaa aaaccaacct cagctgtttt tcatgacgat
       61 actgtcgatg gtgttggcca cgcgctcgca gtcgtcggag cactgttcga ggtgacggta
      121 gacgtcatgc caggcgaaca cctgcagcgg atcggtgcag gtggtgtgca gcgtgcgcat
      181 cgactcgatg aacaggtggt cggcgtccgt ttcgatcgtg ttgatggcga acaccagttc
      241 gcgcaacgtc ttggaatgcc ggaatcgcgg cagttcgttg accagctcgc agatgctttc
      301 cgtggcgcgc accaccatat ccgacatctg gaccgcgtcg tcgcgcatgt cggtcacgtt
      361 gtcgtagtac atgcggttga gcacgccttc gatgctgtcg gtcacgtcgt cgagcacgtg
      421 gccgagctcg tcgatgtcct cgcggtcgaa cggcgtgatg aacgcggtca ccagttcgtc
      481 catcatgtcg tggcgcacct cgtccgctga cttctcaatc gcgtgcatgc tgtccatgcg
      541 ttcgcgcagc tggcccggat tgaagtcgtg catgacctcg gacagcagct tcgcggcctg
      601 gcatgcgtag tccgcgctgc ggcggaatcc gtcgaagtag aacgtgtctt tttgtctcgc
      661 catgatgggt ggattccttt ctagaacacg accatgaaca gtttggccat tacgaagctg
      721 atgagaccgc aaccagggaa ggtgaacacc caggtgagca tcatgtcgcg caccacgccg
      781 aagttgatgg cggagaggcg gcgtaccgcg cccacgccca tgatggcgct ggtcttggtg
      841 tgcgtggtgg agacggggat gccgagcacg gtcatgacga gcaggcacag cgcgcttgac
      901 agatcggccg agaagccttg gtatttctcg agtttcacca tgtcctggcc cacggatttg
      961 atgatgcgtt caccgccgac ggacgtgccc acgcccatga tggtgctgca cagaatcatg
     1021 agccagatcg gaatcatcac accggtcacg ctgggttggc cattgcagaa cgccatgccg
     1081 aggaacagca cgccgatgaa cttctggccg tcctgcgcgc cgtgcatgaa gctcatggcc
     1141 gccgcgccca cgatctgcgc gtaggtgaag aacccgttgg tgcggcggcg gtccatgcca
     1201 gcgcaaatca gggtcacggc cttgcatacc aaccagccga cggcgaagcc gaacaggagg
     1261 cttgcgacca gaccgtagag caccttgacc cattctccca tgttgatgcc gccgatgccg
     1321 ccttggatgg cgatggccgc tccggacagg ccggcgatca ggctgtggct ttcggaggtg
     1381 ggtatgccga gcagcgaggc gcccacgctg tatacgacga tggagaacag cgccgcgcat
     1441 agggcgatga gcgccgcgtt cgtgtcgccg ccgaaatcga ccatgttgct gatggtggag
     1501 gccacggagg cgttgagctg cgtcatgatg aagacgccga ggaagttgaa gatggccgcc
     1561 atgatgacgg cggagcgtac gcgcatgcat cgtgtggtca cgcaggtggc gatggcgttg
     1621 ggtgcgtcgg tccagccgtt gacgaagatg actcccagcg tcagcaacgt tgtgacggtc
     1681 agagccgggt tggaaagcac ttgttggagg aaatgcgcca gtgagacgtc caaggggaat
     1741 taacaccttc ttcttcgggg gcggaagccg tcacactgat tgtaacggaa gaatgtctac
     1801 gtagttccca tggcgcgcgg gttttatggt gttgttatgc tattgcggcc tgtcagcagc
     1861 aacaactggt attggggtgg agacggtcgt attcctcgcg ggcgcgaatg aattcgacct
     1921 cgctcatcgg tgtttcgccg ggatgctcgg ccgcgaaatg ctcaagataa tggtcgtagc
     1981 gggcctcgcc gctgatctca cgcagatacc agacgatgcc gtgccatacg gcaacgagtt
     2041 tgccgagcat tttgtctcct ttcgctgatt attggctccc cttgaaaaaa ggggagccaa
     2101 tgacgcacta cgctgcaacc tgcccgttcc gcacaagctc atgcagcttg gcgctgtatt
     2161 cgcgctgcac cttcttctca agcgcggtgg cgaccaggct ggagggcgcg aaccactggg
     2221 attccacgaa cggctcctcg gacgtcgttt cggcaccgta cttgccagcg gcgaacgtgc
     2281 gcaccacgac cacgatgccg actaccacga agacgcccat catgaccagg aagaacacgg
     2341 agagcacgcc gtccagatac gcgttggaca gcgcggcctt ggcgttggtc agcgcctcgc
     2401 cggtcagctc gccggagtcg atctg
//
LOCUS       DAWBMW010000221         2405 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_8521_length_2405_cov_2.548936, whole genome shotgun sequence.
ACCESSION   DAWBMW010000221 DAWBMW010000000
VERSION     DAWBMW010000221.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8521_length_2405_cov_2.548936"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1421..1430
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          459 a          655 c          726 g          555 t
ORIGIN      
        1 atacgggtga gtgtggactg tccgtcggtt tttgccagac gcttgtcaag cactttcgca
       61 gtggagcctt ccttagcggc atcgtaggcc acggcttgcc aatcgttttc cgtggatttg
      121 ccgtccaagg tgcctgaagg gctttccaca acgcccttct gatagtagga gcccagtacc
      181 tgccagatac cacggaacgc ctcttgggcg aacgtggaat cgttggcgtt gcctttttcg
      241 tccatggcgg tgtatggcag cagcacctca atgtatttgg ggttgtcttt actggcctta
      301 tccaacttga gtttggacac catcttttcc gcctgtagct tgccgatggt ctgggcgtcc
      361 gagaacttga cgaaggcgtc cggcctatac ccctcgatgc tgttgccgag caataccaca
      421 tgcatgccgg attccttggc cagcttgagc gatgagacca gccggtccac ggcctgcgcc
      481 tgctcgtccg atgcagtgct gtcgctctca gtgttggacg agtcatcttt ggaagagctg
      541 tcttcggaag aatcaccgtc agaagtagct ccctcatccg tgacatcgtt ggtgctcagg
      601 ttctggctga tgtaatcgcc gtattggcga gtggaagcgt cggcctcgac tactggagcc
      661 acgagcaatg tcatgttgtc gggcgcgatg gcaatgccgg aatcgtccgt ggtgttattc
      721 gaaagatgat cgaccacaaa gtcttggatg gcgcggcttt gcttggccaa cgagtcggac
      781 gtcgtatgat cgatatcgcc tttcttgaaa ccggcgtcgg ccagagcctt ggctaggtca
      841 ggcaccagct tcgcccattt gttcagtggc gtctgctggt tcagcgtaat gccgtccgaa
      901 ggtgtgaaca cggccacgcc gccggcagaa ttggacgtca ccgtggtttg accatctaca
      961 acggtcttat tgtctgaggt gcacgcggtc agtgacagca ccatcgcgca ggatgcgata
     1021 atggcggcga gcagggtggt tgagcggcgt gcgaacacca taagcggcga aacctcctca
     1081 agcgggcgtg aaaacaagct gaatcacgat tctagtaggc cgtatacgaa aaagtcccgg
     1141 accagtggac aaatcgccac atatccggga acaattagag gtattacgtg cgtgcatcga
     1201 acaactgtca tgattatcgt gctgaggaac attccagatg aagcacgaca atcatgacag
     1261 tttgcgacat gacatgaccc atgtaggtcc gcacgatgtg ttcgatgatt tactcggcca
     1321 gagccttggc caggttctcg tccagcgcgt tcatgaagcc gtcggtgtcg agccacggtt
     1381 cgtccgggcc gatgagcatg gccagatcct tggtcatctg nnnnnnnnnn gtgccgtgcg
     1441 ccgcttcggc ctcgacggtc tggccgtccg gggtcatgag cacggaggtc atgaggccca
     1501 gcgaaccgaa gccctgggcc acggagtcgg actgcacatc gccatcgtag ttcttgcagg
     1561 cccagatgta accgccgtgc cacttcaggg agctggccac catgtcgtcg atcaggcggt
     1621 gctcgtaagt caggccggcc tcggcgtacc tgtccttgta ttcggtctcg aacacctcgg
     1681 caaagatgtc cttgaactgg ccgtcgtagg ccttcaaaat cgtgttcttg gtggacaagt
     1741 acaccgggta atgacgcatc aggccgtagt tgaagcaggc gcgagcgaaa ccgcggatcg
     1801 aatcgttgac gttgtactgc acctgggcca cgccgccgtc ttcgccgtag ttgtagacca
     1861 cgtgctgaat cggctcggag ccatcgtccg gagtgaaggt gacggtcagc tggccggctc
     1921 ccggaacctt gaaatcggtg gccttgtact ggtcgccgaa tgcgtgacgg gctacgacga
     1981 tcggcttggt ccagccgggc accagacgag gcacgttgct catcacaatc ggctcgcgga
     2041 agatcgtgcc gcccaggatg ttgcggatgg tgccgttggg ggacttccac atcttcttga
     2101 gaccgaattc cttgactcgg gcctcatcgg gggtgatggt ggcgcacttg acaccgacat
     2161 ggtgcttctg gatggcgtgg gcggcgtcga tggtcacctg gtcatcggtc tcgtcgcggt
     2221 gctcgatgcc cagatcgtag tagtcgaggt tcacgtcgag gtacggcagg atcagacgat
     2281 ccttgatgtc cttccagatc acgcgggtca tctcgtcgcc gtcgagttcg acgacagtgc
     2341 cttcgacctt gattttggcc atgcttcctc ctgatagatg gtgtgaagat gatttagtca
     2401 tagcg
//
LOCUS       DAWBMW010000222         2386 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_8605_length_2386_cov_2.983698, whole genome shotgun sequence.
ACCESSION   DAWBMW010000222 DAWBMW010000000
VERSION     DAWBMW010000222.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8605_length_2386_cov_2.983698"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          456 a          664 c          769 g          497 t
ORIGIN      
        1 cgcgtagcga gactgagggg agcgtcaacc gcccacctca attgtggata acttcataag
       61 ctagaaccac ataccccgtt tcagctggac aaacgcccat gaatccatca gcgtggatgc
      121 atgggcgttt ctcataacca tcgcgcagac catgccgcca accgtttgcg cgcattcagc
      181 aggcagagcg cagccatgcc ccaaccgcga gtggaacggc ccacgacgcc tccgccgcct
      241 cctggccatg cacggatccg tccggctggc cacccgcccc aaagggcgca gagcggcctg
      301 cggcaggcga cccgtgacaa acgtgcgtta tcggatctga ttggcgtacg ttccgccgat
      361 gccgtcggcg aatcagccag actcacccgg gatcagccgc gattgtcgtt caccattggg
      421 catgccttgg ccgtgatatt gctgctgatc gtcgcccttg gcatcagctt ggcattgctg
      481 gtccaacagt cgatgaatat ggccatgctg tctcaaccga ccgccatgca gaccacgggc
      541 ggctccgctg gagatcagac gatgccatcg cagaacacca cggacgagaa acgcacggat
      601 ccgcagcagt cggagcattc cgatgccaca gcgaaccccg aatcggatac cacgcaatct
      661 ccgcacatgc agacggacgg cagcgatgcc ggcgcttcca cgcatggtct tatcaacctc
      721 aacacggcta ccgctgagga attgcaatcc atcaaaggta ttgggccggt taccgctcag
      781 cggatcattg atcatcgcac ggcgattggc gggtatacat cagtcgatca gctgctggat
      841 gtcaaaggca tcggctccaa gacgttggag accatgcgag ggcaggtgac ggtcggatga
      901 gcagggattg gatactgcgc gagcagggga gccgcgattg gaggatgctg ccgatcgcca
      961 tagtgatgtg ggcggcgagt ctgggagcgc acgcattatt cgcatggagg atgtctgatg
     1021 gactcggcac cggtcaggca aaggctgatg ccggagctgg cattgacgtc accataatcg
     1081 gcatggaatg gcctgtcaca cgaatattgc ctataacagt ggcgtcatgt gctgccgtgg
     1141 cggcgatgat tgtggtgtct ttccgcctgc ggatccggtg gacgggaaca ttgaccgtat
     1201 gcgttgcggt ggcgtgcatc ggcagtatga cggcaatcga gtcggacacc attgcctggc
     1261 atgatcctgc ctccgcacaa gctcgccaat ccagttcata ccacgaagtc atggccacgg
     1321 tgacgacgcc cgtgatcgct tcggaccaac gcacatacga ttgtcagacg gacatacggc
     1381 tgagcggcat cactgtggat ggtacggatg tacgttccac ggtcgctgtg cgtgtatacg
     1441 ccgacgaatc gtattgtggg cagttgcgtc gaggcgcggt ctatctgctg acaggcgtat
     1501 tgcagcaagc gcgatatgga cgcataccac tatggctgct cttggaagga aaacagccgg
     1561 tagcacaggt gcgggctccg ccgtggcatc tcgccgcgat tgcgcatgtg caggaggcgt
     1621 ttttcaccgt gaccgaacaa ttgtccgatc aggggcgtgt gttggtgccg ggattgacga
     1681 tgggtgtgct tggccaggat tatatcggcg gcgagaccgg tccgatgccg gtcaacagca
     1741 cgtatgcgca aacgttggag aatcagttcc gccagtccgg catcatgcac ctcatggccg
     1801 tatccggcgg ccactttgta ctggtggcgg gattggtgcg ccggttatgc atgtggatgc
     1861 tgcttgatcg gcggctgacg gcgttgctgg tctctggagt atatgtgctg ctggcattgg
     1921 cgatgttccc cagtgattcg gtcacccgcg cgttcatcat gggattgata ggtgcgttga
     1981 cgtatgcgat ggggcggcgt acgcaagcgt tgagcgccct gtgctggacg gtaatcggtg
     2041 tattggcggt gaatccggat atgtcggcca gctacggatt cgccttgtct tcggccgccg
     2101 tactgggtat cgtgctgttt gcggggcggc tggccggtgc ctttgagcga gcgatgccgc
     2161 acggcatcgc cgaaatgacg gcgatgaccg tggccgcgca gttgttcacc ctgccgattc
     2221 aggtgttgat ggaaccggag ttgccgttgc tgtccgtgcc ggcgaacctg ttggtgtcgc
     2281 cgtttgtggg attggccacg atggcgggat tgatggcatt ggcctgcgca tggtgcgagc
     2341 catggctggc cggcgtattc gcgtggatat cgtcatgggg cacgct
//
LOCUS       DAWBMW010000223         2349 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_8778_length_2349_cov_3.044464, whole genome shotgun sequence.
ACCESSION   DAWBMW010000223 DAWBMW010000000
VERSION     DAWBMW010000223.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8778_length_2349_cov_3.044464"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          533 a          691 c          705 g          420 t
ORIGIN      
        1 cagacgaccc tgctcatcct tggtggcgtc cgggtactgc tcggtcttga cgaagtcctt
       61 gacggtgatc aggccggtca ggtggccttc ctcgtcaacg agcggcagct tctcaacctt
      121 gtgctgggcg agcagacggt gagcgtcgtc cttggaaatg ttggacgggc cagtgaccag
      181 gttctccttg gtcatgacat ccttaacctt cagggtgtcg taatcctcgg aggcgatgaa
      241 gcgcatatca cggttggtga tgatgccgac gagcttgttt tccttgtcca caaccggcag
      301 accggagatg tggaacttgc cgcacagctt gtcgaggtcg gccagagtga cctcggggtt
      361 gacggtcagc gggtcggtga tcataccgga ctcggaacgc ttgaccacat cgacctgagc
      421 ggcctggtcg tcgatggaca gattgcggtg cagcacaccg ataccaccgt tacgagccat
      481 ggcgatggcc atctcggact cggtcacggt atccatggca gcggagagga ccggggcctt
      541 catcacgatc ttgcgagtca gatgagtgct ggtgtccact tcggagggaa tgacatccgt
      601 ctcgttcggc agcagcaaca catcatcata agcaaggccg agcttggcga aaatcggagg
      661 aaggggagca tacgccgatt gggcgttcaa ttcatcgagg tttgtagcca tacaaacact
      721 atatgaacac gcgctgacct tcacgacaca cgcttgtgag cgctcactta tccgctgccg
      781 ggtcctgcac ctcacccgcc tgcgagtccc gcgaatccgc aggcgaatca gtcatcgaat
      841 ccgctgcctg agagccaatc gaatcccggc tctcccctac tgcagactgc gacttaaccg
      901 tagcatgctg cgcgttcttg cgtgcggatt cgagcgtcag gttctcggct tggatctcgg
      961 gaatacgatg ccgcagatag gggtacatcg tggctatggt cagcaccacc acggcgatgg
     1021 gcatgcccac ggccacatgc tgcgctttga agaacagaat cgtcagcgaa ccgaatgcga
     1081 tcagcgcggc ccatccccac aggatcagca cggcgccacg caccgaatgc ccgatcttca
     1141 gcatgcgatg gtgcaggtgc atacggtccg gatgcatggg cgactggcct ttggccaaac
     1201 gccgcacaat cgccagacac atgtcgagca caggcaggaa caacaccaga atcggcagca
     1261 gaattggcat gaacaccggc agatagatgc tggcatgaat cgaggccggg tcaagacgtc
     1321 cggtcatcac aatcgaagcg caggtgatga gatagccaag cagcatcgaa ccggaatcac
     1381 ccataaacag tttggcggga tgccaattat gcagcaggaa gcccacgcag atacccacca
     1441 tcatcacgtc gatcagcgtg gccaacgaag cataactcgg ggaagaccgg gcgatgatat
     1501 acgaataggc ggcgaacgct atgccaccaa tcgccacgat accggccgcc aggccatcga
     1561 ggccatcaac gaaattgacc gcgttaatcg aggcgacaat caggaatgcg gtgatcgcca
     1621 tggacagact cggcgaggcg gtaatcagcg aaccgcccaa aggcagtgag atgatctgca
     1681 atccgcccca agccacgaat acggaaatca gcagctgacc ggcaagtttc agcatccaat
     1741 cgagatccca caggtcatcg gccatgccca gcagactgat catgatgccg ccggcaagaa
     1801 tcacccacat ctggtaattg ccgttgaaca atcccgagag aaaaggcagc cgactggcga
     1861 atacggtagc cacggcaaaa ccgatcagca tgccaaggcc acccatgcgc ggcgtgggaa
     1921 tggtgtgcac gtctcgggca cgcacctcgc caaccgcacc gatttcgatg gcgacgtggc
     1981 gaatcagcgg agtcaccagc caggtgacgc caccggcgat ggcggcaatc agtaggtaga
     2041 ttctcatgcg ccgagtccac cgccgttgag gtggagggcc ttgcgaatca cgttttgggc
     2101 gatgacgcct tcacgcagga tctcgatgcc gtcgcgcgcg tacggatcgg ctttgacgac
     2161 ggtgctggag acatggcctt gggtgggacc gccgtccaga tacaggtcga cttcgctgcc
     2221 gaacgcgtca accgcctcct ggacggtctg cgcgctttcg tcgccggaac ggttggcgct
     2281 ggacgcggcc agcgggccag tcgccttgag aatcgcgagg cacagcgccg aattggggat
     2341 gcggatgcc
//
LOCUS       DAWBMW010000224         2349 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_8782_length_2349_cov_1.722755, whole genome shotgun sequence.
ACCESSION   DAWBMW010000224 DAWBMW010000000
VERSION     DAWBMW010000224.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8782_length_2349_cov_1.722755"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          479 a          696 c          714 g          460 t
ORIGIN      
        1 gtggagggct tgtcgaacag tacggcgacg gcctgcggac cggcgaacgg ctgcttgtag
       61 aagcggtcgt ggtggaactt gatggcaagt tcgagcacct gcttctgttc ctcatggttg
      121 aggtcgtcgt cacgcagcat gtggcgaagt tctggggtca tgatgatctc cttggatcgg
      181 tgggtcggca gtgacagaga gaaatcagtc gtcgggcagg tccgtaggca catccttgag
      241 gatggcaagt gcctggtcca cgtcttgtgc ggttacaatc agcggcggtg cgaaacgcag
      301 cgcattggga gccaccgcgt tgacgatgag cccgtgttcg aggcagtagt tcatcaccgc
      361 gtgggagcag ggatgcttga gctcgacggc gtccagcaaa ccacggccgc gtacggaaac
      421 gaacagcgga ttaccggtgg ccataatgcc atcgcgcagc tgttcgccgc gagcttcggc
      481 gttggccacc agattctcgt cttcaatcac gtcaagagtg gcgagtcccg cagccgcacc
      541 cagcggattg ccggcaaagg tggagccgtg ggaaccagga gtgaacagag ccgcaagttt
      601 ctcgccgaaa gcgatcatgc cgcccatcgg gaatccgcct gccacgccct tggcgaaagt
      661 caccatatcc ggcgtcacgc cgccggacag atcgtcacgc tggaatgcga accacttgcc
      721 ggtacgtccg ataccggtct gtacttcatc gatgataagc agcgcgtggt tgatatcgca
      781 cagttcgcgc acgaacttga cgtaatcggc gcccaacggc tgcacgccgg cttcgccttg
      841 gataagttcc agaatcacag cagccaccgg acccttgcca tagcggccga gaccggtctg
      901 tgcaaaagcg tcgtgcaggg cgaccttgtc gccggcgcgc acgaattcga tgttcggcac
      961 cagcggatca tacggcttgc ggataccggg tttccacgtg gcggacagcg cacccatcgt
     1021 acggccatgg aagccgtgag tcattgcgag aatgcgtgcg ggcttgccgc cgatggaagg
     1081 cagggcacca ggcaaggtgc gtccatacag cttggcgagc ttgagggcag cttcattgcc
     1141 ttcagcaccg gagttgccga agtagacttt ggagccttca ggtgcaccag ccagcttgac
     1201 cagttttgat gccaattcaa tttgcggctc ggacgcgaaa tagttgctga tatgagccac
     1261 cttggcagcc tgatcggcaa ccgccttgac ccacttgggg tgggcatatc cgagggaatt
     1321 gacggcgatg cctgccagga aatcgaggta ttcgttaccg tcaacatccc agatgtgggc
     1381 accttggccg tgatccatga cgcgcagcgg cgtaccgaac acattcatgt ggacctgaga
     1441 gtactctccc agccacttgc tgtcttccgt acctagtttt tcctcatgct cagtagccat
     1501 aagagctcct catttccagt ccttcatcgg gcataaccat ggtaccgatg cctgcggagg
     1561 tgaatatctc gttcaaaatc gaatgcggct tgcgcccatc gatgatatgg gcttggggca
     1621 cgccaccgtc gatggccctg acacaggcct ccatcttggg tcgcataccg gattccaggt
     1681 ccggcagcat atcgcgcagg gtttccacac cgatccggcc aatcagagaa ttcttgtccg
     1741 gccaatcggc gtacagacca tccacatcgg tcaagataac gagcttgtgt gcgcccacag
     1801 cggcagccaa agcggcggcg gcggaatcag cgttcacgtt gagcacttca gtggcatctt
     1861 cttcgttcgg agccacggaa gagacgaccg ggatacgacc ggcggccacc agatcctcga
     1921 ctgccgaagc atcgacggaa accacgtcgc cgaccaggcc gatatcggtg ggcttgccat
     1981 cgatgatggg cctgcgctgc atggccgaga acaggccgcc gtcttcaccg gacatgccta
     2041 cggccagcgg gccgtgcgcg ttgatcaggc ctaccagttc gcgtgaaacc ttgccggtca
     2101 gaaccatgcg caccacatcc atggcctcgg gggtggtgac cctcaggccg cctttgaatt
     2161 cggatttgat gccgagggcc ttgagcatgt gggagatctg cggaccgccg ccgtgcacga
     2221 cgatcgggtg cagacccacc tgacgcagaa agaccatgtc ttcggcgaag cactgcttca
     2281 gatggtcatc gaccatggca ttgccgccgt atttgacgac gatacgctgg ccggcgaact
     2341 cctccagcc
//
LOCUS       DAWBMW010000225         2330 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_8852_length_2330_cov_1.678681, whole genome shotgun sequence.
ACCESSION   DAWBMW010000225 DAWBMW010000000
VERSION     DAWBMW010000225.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8852_length_2330_cov_1.678681"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1323..1332
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          478 a          714 c          698 g          430 t
ORIGIN      
        1 cgggtcgtgg accggcacgg cgcgcatcga tccgccggtg gcgtcgacaa gcgcgcgtgc
       61 ggtgccttca ccgccgtcgg ccatgggaag gcaacggacc tcggcatcgt ggtcggcatt
      121 ctcaatcccc tgagccatcg cccgggccgc ctccatggcg gtcaggctct ctttgaagga
      181 gtcaggggcg cataagtatc gcgtcatgct tcgagcatac cgcacgacac gacgaaacgg
      241 taaacgtttt ccgtaattcg cgtgattatt tttttgttca gaattcaggc tttccaatcc
      301 acaatgctac tattgcaagc aaaaatgcga tatcgttgcg gtaaacgttt agacacgttt
      361 tccgtttcag ggaagaaaag gatcgattcg atcgtggctg tacagaacga taaggcgcaa
      421 tcgccagcga cattgatgga tgtcgccaaa gaagcccaag tctccatcgc caccgcatcc
      481 cgcgtgctca acggcagcac ccgcaaggta cgcaaccaat cgtatgtcaa ggtgatggaa
      541 gcagccacca agctcaacta tcgtcccaac gcctatgctc aggcagtggc caaaggcacc
      601 gcaccgaaca tcgtgctgct ggtctccgat atctccgatc cctacttcgc atccgtatcg
      661 caaggcgtgg tcaaggcggc ggccgaacgc ggcatttccg tgaccatcgg cgtggcagcc
      721 accccgcacg aagagttgga ggcggtgcgt aagtccgtat ccaccaggcc gcgcggcatc
      781 atcatctccg aaagtgaata caccgacgcg ccggaacgcc aggcgttgca gacggaactc
      841 acccagtacg aagcatccgg cggacgtgcc gtgttcgtgc tcaaccgcaa cctgcccttc
      901 ccggccgtgg acgtggcgaa cgatgtcggc gcgcgcgcca tcgcaaagct gatcgtcgat
      961 tccgggtata cgcgccctgc gattctcaag ggcgatgacg cacatcgctc ctccaaagaa
     1021 cgactggccg gcgtgatgga agtgttggac gaggcaggca tcaccgtgga tccgaaagcc
     1081 gtggcgaatg gtaagttcag ccgtaccgac ggctatgcgg ccgtcatgca aatggcccga
     1141 tccggactgc tgaagccggg cgaaggcgca ctgtacgacg gcaaaggcat cgattcaatc
     1201 atcgcgctca acgacgtgat ggccatcggt gcgatgaccg cgttgcgcgc caacggcatc
     1261 gagccgggtc gggaaatcgg cgtcaccggt ttcggcgaca tcccctactc cgccgacgtg
     1321 ttnnnnnnnn nnccgctggc cgaaatgggc caggccgccg tggacagcat cctgtccaac
     1381 cgcgatgacg gggaggccac cggcggcact gccaaccgcc tgatctacgt gcaaagcgcc
     1441 ccggacgtag tcctacgcgg ttccctcccc cgccggtaaa acaaaaatag ctccctctga
     1501 cggtgtaacc acaactagcg aacaggtaat ggcgactgcc gcgcaccata ccaccgactc
     1561 atacaccgac gaaatcgaag aactgttcga ttaccgagtc attgagcaac cggccacgac
     1621 gagtgggcac cactcgatga ttatcggata cggtaatcag gccttcgctg accattgggg
     1681 cgagttggtc aagggaaaca ttacggagag tctggggcgt gctgctcata cccgcatcgt
     1741 tgatggcgcg gttgatgcgg tcgaggtcaa ggccctcgtg caggcgcagc ccgagcatga
     1801 tgagctcttc gaggttttcc tcgggagtga tcgtctcgct atcggcccac ggcacacggt
     1861 tctcgttgat ggccgtgccc cacaaacgcg ggtgcgcgat gtcccagctg cgcagaccgt
     1921 gtgagacaga aaccaccccc agtccggcat ccgccggaca acccccgcca gcgggggcat
     1981 gaatttcttg ggagttgttc ccatgcgcgt tgatcatacc tagttcggcg ttggcctgtc
     2041 catcagctca ggcagaggca atggcttcgg acttgctccc gccagcgggg gctgtcagcg
     2101 aagccgactg ggggtggtca gcctcggtca ccgcgttgta gtgggagtgg gcacctggcc
     2161 ccaggccggc ccaatcgacg ttgcgccagt agccgaggtt gtgctggctt tcgtatccgg
     2221 ggcgagccca gttggagacc tcataccatt cgaggccggc ggcggcgaac aggtcgtcag
     2281 cgatctcgta tttagcggcc tcgtcatcgt cattgggctt gggcagagtg
//
LOCUS       DAWBMW010000226         2318 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_8917_length_2318_cov_1.794079, whole genome shotgun sequence.
ACCESSION   DAWBMW010000226 DAWBMW010000000
VERSION     DAWBMW010000226.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8917_length_2318_cov_1.794079"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    600..631
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1381..1390
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          421 a          709 c          669 g          477 t
ORIGIN      
        1 gcgggcccgg gacgcgagcc tggtattggg cgcgttcgcc acgctcgact tccctctgac
       61 cgaggtggag gtgttccaca cggaccgggg cagcgagttc gacaacgcga aaatcgacga
      121 gctgctcgac gtgttcgaca tcagaagatc gctgccaagg aagggcaacc cgtacgacaa
      181 cgccgtggcc gaatccacca acaggctcct gaagaaggag ctcatatacc ggaaccacta
      241 caccagcctg gaacaactac gctccgaccc cgacgactat gtgtggtggt tcaacaacca
      301 aagactccac tccaccctcg gataccggag ccccaacgaa ttcacacaac aaggactcgt
      361 cctctaaaga attgtccaac acgccgttgc caatccagac ccacggggac accagggccg
      421 gacgcgtgac gacccatccc gaccggtgat atataacccc ggaacaggcg gaatcacatg
      481 cgatatgttc ttctggggtg tgctcggcct ggcatgggtc aagctgatgc tgccgtggct
      541 gctgcgcctg atccaacgca ttccatggaa gatccgccat tcgctgaccg ccgtgtgccn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nacacatatt tcaacgatga tttcatggcg
      661 gaacgtttcc agacgatgtc gctggatccg ggcaaggccg gccgcctgtg acgccgggga
      721 cggccgcggt gggtgttagg aaaccgttaa caatccgtcc gcgggcgtat gggttccgtt
      781 agattcggag catgacttcg ctcagggcca tatcatcaag atgcttattg gaaataggtc
      841 cggccgcgcc cgcgaacgcg gcggcggccg gaccgtgcat ctgatgattg gagaagagca
      901 acatgccttg gtgggttgat gccgcctatg tcgtgggcgt aatggcgctg ttcattgtct
      961 tcgacctgat cgcgaaggcg gtggatcgtc tatgatggct gttttctgcg ccatcggcgt
     1021 cgtattggga atcgccggtg tggtgtatat gggggtcgcc ctgtgccgcc cggagcggtt
     1081 ctgagggccg gggaagagtc ttaaggttcc gaaatggtat atctctatgc ttttatggcg
     1141 ttcggtttga tcgccttgat cctggcggcc atataccggc cgttgggcga ttacatgtat
     1201 cgcgtgtaca cgaccgataa ggatctgttc ttcgaaaagt ggatctatcg catcatcggc
     1261 gtgaatttct cgaagggaca gtcctggaag gcgtatttcc gcggagtgct tggcttctcc
     1321 gtgatgagcc tgctggtcct gtacctgctg cagcgcgtgc agtcctggct gccgttctcg
     1381 nnnnnnnnnn gcttcgacaa cgtgccggct ccgctggcgt tcaacacggc cgcatccttc
     1441 gtgacgaaca cgaactggca gtcctattcg cctgaccaga cattgggcta ttccgtccag
     1501 atcgcaggcc tgtgcgtgca gaacttcgtt tccgccgcgg tcggcatcgc cgtatccatc
     1561 gccctggtgc gcggcttcat gctgcgccgc tccgacacga tcggcaattt ctgggtggac
     1621 ctcatccgcg gcacgttccg catcctcatg cccctggcca tcgtgatggg caccatcctc
     1681 atcgcgctgg gcgtcgcaca gaacttctcg ggcttcacca ccgtcgccac cgtggctggc
     1741 ggcgaacaga ccatcatggg cggcccggtg gcctcccagg aggtcatcaa ggaactgggc
     1801 accaacggcg gcggcttctt caacgccaat tcgtcccatc cgtacgagaa cccgaacatg
     1861 tggaccaacc tcattgagat cctgctgatg ctggccatcc ccgtcgcgct gacccgcacg
     1921 ttcggccgca tgatcggcaa catccgtcag ggctatgcgc tgctcggcgc catggtcgtc
     1981 ctgttcggca tctccttctt cgccgtcgtg agcttcgaat ccttcgtgga cgatccgctc
     2041 acctccctca ccggaggctc catggaaggc aaggaggacc gcttcggcat cgtctggtcc
     2101 gcgctgttcg gcaccacgtc cacaagcacg tccaccggcg cggtcaactc catgcatgat
     2161 tcctacaccg cgctcggcgg catgatgtac atgttcaaca tgatgctggg cgaagtctcc
     2221 ccgagcggcg tcggcgcagg cctgtacggc atcctcgtca tggccgtcgt ggccgtgttc
     2281 atcgcaggcc tcatggtcgg ccgcacgccg gaatacct
//
LOCUS       DAWBMW010000227         2286 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_9053_length_2286_cov_1.912147, whole genome shotgun sequence.
ACCESSION   DAWBMW010000227 DAWBMW010000000
VERSION     DAWBMW010000227.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9053_length_2286_cov_1.912147"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1422..1431
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          409 a          653 c          718 g          496 t
ORIGIN      
        1 gtatgggtca tgccctgatg gccggtcatg ccctggacga taacgggagc gccgtcttcg
       61 atgaacagca tcagcgaacc tccttggact gggtcgcctc accggcgagc tgcgcggcct
      121 tggctgccgc gccttccatg gtttcggaca catggatatt cgggttattg gcggaggcga
      181 ggatatgcag accctcggcg gcggcgttgc catcgaagcg cacaacgatc ggcttggaac
      241 cgccgagctt ggcgaccgct tcgaggatgc cgttggcgac ttccacgcag gaggtgatgc
      301 cgccatagac attgatgaac acggattcga cctgagggtc ggacagtacg atctccaggc
      361 tttcggccat gacggctgcg gaggcgccgc cgccgatgtc caggaagttg gcgggcttga
      421 tgttggtgcc ctgttcctcg ccagcgccgg agacggcatc cagggaactc atgacgagtc
      481 ctgcaccgtt gccgatcaca ccgacctcgc cgtgcaggtg cacgtagtgc aggccgtgtt
      541 cgcgggcgcg ctgttcgaag gggtcgggaa cgatgggatc cacgaaacgc gcccagccgt
      601 catggcggaa ggaggcgttg tcatccaagg agatcttggc gtccaaggcg ctcagctgct
      661 tggtggattc gtcatccggg tcgccgatct tggcgagcgg attgatttcc acgagcgtgg
      721 cgtcgttgtc tttgaagcag cgccacatct tgagcagaat ctgtgcggcc tgatcgacgt
      781 ccgcgtggta gaagccgatg ctctgtgcca tttcggtggc agcggccagg tcgaagtcac
      841 cgagcgcatc gatgtgcagg cgcttgacgg cctccggatg ttccttggcg atttcctcga
      901 cttcggtgcc gccgttagcg gtggccagca cgtcgaagtc gcgcgaggtg cggtccacgg
      961 agatggacac gtagtattcg tgcaggatgt tcttggcttc ggctacaagc acgccggaaa
     1021 ccttgtgccc gtggatggtc atcggcagga tggactcgga ttcaagaatc gcctcgtcgc
     1081 ggttatgggc gatcttgacg ccgccggcct gaccacgatg accgatcttg acctgcgcct
     1141 tgattacgca gggatagccg attttgtccg ccgcttcagc gacttcatgg gagttctggg
     1201 caaagatggc gtccggggtg ggaatgtcct gttcctcgag cagctgacgc gcctggtatt
     1261 cgtaaagatc catcgatcct tccctgacta tgtaattggt ccggcatgta actgtacaca
     1321 gtacatgccg gaccttgaaa actgactgta cgttgggtga gacgaaatct caggccggca
     1381 tcgcgatcag cgagctgctg ggcttgccgt cgagcttatc cnnnnnnnnn nagcttatcc
     1441 agaccgtcga ggccatcgag ccgcatcacg aaactgaagc ccaccacggt gccaccggcc
     1501 ttttcgataa ggtcggtggc ggctttggcg gtgccgcccg tggcgatgag gtcatcgacg
     1561 atgagcactc gcttccctgc ctgtacggcg tcggtttcga tttcgacact ggccgtgccg
     1621 tattcgaggt cataggattc accgatggtt tcaggcggca acttgccggc cttacgcacg
     1681 gcgatgaaac ccttgcccaa atgggcggcc atggccgggc cgaacaggaa cccgcgcgat
     1741 tcgagaccgg caatggagtc gaattcgctc ggggacaccg gcagggcctc ttcaagtgct
     1801 ttgagcagaa tcttcaagcc cttggggtcg gcgagcactg gcatgaaatc acggaagatg
     1861 atgccttcct tgggaaaccc cggtacggaa cgcaccaagg acaccagata ttcggcgtcc
     1921 tgttgcccta ccttgctcaa tgcgtcgatg gtgatgtccg actgtgccat agtgctcgcc
     1981 ccttttatgc ctttgcgatg cgatacgcgg atggtgctac cgaatcgaac agcttacttc
     2041 gtcttggttt cgttgtcgtc acataggccg ggcgggtgta ctcacgggcg atggcgttga
     2101 agccgaaacg cagcttggag ccttcggagt cgatgacgat ctcctcatac tgctcgtcga
     2161 cttcgacgac cttgccgatc acctggccga tggtgatgac ctcggtgcca ggctgcaggt
     2221 tgcgacggaa gtcctgcttc tcctgctgtt gctgcttggc cttcttggac tgccaccaca
     2281 tcatgc
//
LOCUS       DAWBMW010000228         2278 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_9083_length_2278_cov_2.253711, whole genome shotgun sequence.
ACCESSION   DAWBMW010000228 DAWBMW010000000
VERSION     DAWBMW010000228.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9083_length_2278_cov_2.253711"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          407 a          701 c          612 g          558 t
ORIGIN      
        1 acgtcatcct aggtgaatga ataagcgctt aggtatttta aggggccgat gtcgcaattt
       61 cgtgagaagg aagtgcttgc ctatggagaa cgtcaggtct ccctcatttc ttcgggaatc
      121 ggccattttg tttacgagaa ggaagtcaag taacccttgc acttaccggg cggagtgttc
      181 ttcccttttt cccgctccgc ctggttcctt cccatcgcgc gcacgacgtt gtgtgcgatg
      241 ttcgattccc agttcacacc aaaccagcaa agcgtggtga caatcatgga ttcccataag
      301 gcagccaagg cagtcaagcc gaagcgcaat atgtccctcg gtctggtcag cctgctcttc
      361 cttgctccgg cgcttgtgtt ttacatcgcc ttcgagctgt ggccgatcat tcagaccatc
      421 tggtactcgt tctacgagtg gaacggtatc gatgctagca ccttcatcgg tgtcgaaaac
      481 tacatcaccg tgttcaccga ccccgatctg tacggatcga ttctgcactc cttctacctg
      541 atcatcttct tctccatcat cccgattgtg ctcggcctga tcattgccgt gctcatcaaa
      601 gacatgaagt ccaaggtcgg tcgttccttc gcgcgtgtgg ctcggcgacc aatccacctc
      661 cctgaacgcg gtaggcgtga ttggtttctg gctgcagctc ggcttctgtg tggtgctctt
      721 gctctcgggc atcggcggca tcgaccagtc cctgtacgag gcagcaagtc ttgacggtgc
      781 cggctggtgg cgtcagctct tcttcatcac gttgcccggt ctgcgcggcc aaatcggcgt
      841 atgcctgacc atgaccatca tctccgcgct cgccagcttc gacgtggtgt acatgtccac
      901 tcagggcggc cccggcacct ccaccatggt gcccggcgtg caggtctacc gcttggcctt
      961 cacccagcag agcgttggct tggcctccgc gctcgccgtc accctgatga ttctcgtgct
     1021 gctggtcgtc ggacctctgc agcgtctggt caacggaaag gagcagtgac catgcagcaa
     1081 tccaaagccg ttcgcatcac cggcacggcg atgctcgctc tgaccgtcct cttctgcgta
     1141 ctgccgttcc tgagcatgct ctccgcggca ttgcagccgc aaggttccat gcccgaaggt
     1201 atccagttca ctgcccaccc gcactgggag aacttcatcg acgcctggaa catggcaaac
     1261 atcaccccgc tgctgctcaa cagctgcatt ctggtgatcg tggtcgtgcc ctgcgtggtg
     1321 atcttctgct cgctcggcgg ttatgccttc gctcagctca aggtgccgtg caagggcctg
     1381 atctacgtgc tgttcctcgt cggtctgacc attccgttcg aaaccttggt cactccgctg
     1441 tactacgaga tcagtggcat gggcttgctg aatacccgtc ttgccctgat cctgccgctc
     1501 atcggcctga atattccgtt cggtattgtg tggatgcgct cctgcttcga gcagatgccg
     1561 cgtgatctga tcgaagccgc atccatcgat ggtgcaggcc atctgcgcac gttccgttcc
     1621 attcagctgc cgttggcact gccagccatc tcctcgctcg gtattctgac cttcctggcc
     1681 acctggaacc agttcctgtt ggctgtggtg ttggagaacg acccgaacaa gcgcacgatg
     1741 gcaggtgccc tacagtcgtt cgttggccag tatcagaccg acgtggtgct gctcaacgcg
     1801 ggcgcactgc tcatcatggc accgacgatg atcctgttca tcttcctcca gcgctacttc
     1861 atccgcgcca tgcttgccgg ctcggttaag ggctgatccg aaggcaagag gatgtagggt
     1921 atggccgccg gaacagtgtt tcgtgcggcc atactggtaa aaccatacgt accgagcaat
     1981 cggacagggg aacgatatga ctttcaccaa caatgactgg tggcgcgacg ccgtcatcta
     2041 ccagatttac ccccgctcat tcagcgatgc caatggagac ggcaacggtg atctccaagg
     2101 cgtgatcgat cgtctcgact atctgcaggc gctcggggtg gacgcgctct ggctttctcc
     2161 attctatcca tcgccgctgg cggatggagg ttatgatgtt gccgactatt gcgatgtgga
     2221 tccccgcctt ggcacactcg atcagttcga cgaattagtg gccaaagcgc acgaacgc
//
LOCUS       DAWBMW010000229         2259 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_9158_length_2259_cov_2.345735, whole genome shotgun sequence.
ACCESSION   DAWBMW010000229 DAWBMW010000000
VERSION     DAWBMW010000229.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9158_length_2259_cov_2.345735"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1144..1153
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          484 a          695 c          620 g          450 t
ORIGIN      
        1 ctacaagctc gcgctcgatg ctgtggcgca cttgctgagc gaatagaacc aagccctcca
       61 gccaatcccg ggtagcagtc gacgttgcgc ccgggattcc tgttacctga ggaaaccata
      121 agcgtccaag taaaggaagc catgactctc accgccacgg aacgtgatct cgtcgatgcc
      181 gcgtcacgct ggtttgacgc ccactgcgca cagttcgtcg atgagctgtg tgagctgctg
      241 cgctacccgt cggtttccga cgaatcggac ccgaatcctc gtccgggcgc gccttatgga
      301 cccgaagtac gccgtgtatt cgaccatatg ctggccaagg caggacgaga cgggctgcct
      361 acgcgcgact acaccggcca cgtcatggaa gtcgtttatc cacaggagaa cgtggagacc
      421 gaccgtgaca tcgccttcgt cgaccacctc gacgtagtcc ccgccgacga cggttggacg
      481 cacgatgcct tcgatccgca ggttattggc gatattgtta tcggtcgcgg ctcattggac
      541 aataaaggcg tcgcgctgac cagctatttc ctactccgtt tcttcaagga acacgaccac
      601 cgtttccgcc accgcgtacg tatactgttc ggcggctccg aagagatcgc gctcaacgac
      661 atcaaatggt tcgtcgccaa catcggcgcg ccgtaccagg ccatcgtcac cgatggtccc
      721 ttcccggtga acaatatcca aaaagggctg ctcgatgtgg atgtcgaact gccgggcggg
      781 ccgcaattgc gcggttggca tgccggcacc gccactaaca cggttcccgg ggccgccgca
      841 atcaccctca ccggcgtcga cgaatctacc gtgcggcaag cattctgcca gagcggcaac
      901 atcgctccag atatcgccga gcgattgcat atcaacgcaa cagcgcaagg cgtcacgatt
      961 gaggccaccg gtgtcgccgg tcatgcctgc cagccgtccg gtaccgtaaa cgctatagcg
     1021 gtgctcacta cggcgcttgc ccggtccggt ctgctcgaag gccgtgacct cactgcagcg
     1081 caggcgattg cccaatggac aaaagactca tatggcaccg gcttgggtat cgattgcgag
     1141 aatnnnnnnn nnnatggtgg tttgattata ccggcgaatg agttcgctga agcagacaag
     1201 gtattaggcg cggtatcggg tgagacttct gaagatgcca tcgtattgca cttcgacatc
     1261 cgttatgcgg taggccaggc acacgaacaa atcatcgagc gtattcaagc acaagccgaa
     1321 gcggcgggcg gccgcattgt cgacatcctt aacgatgacc catactacgt gcaggctgac
     1381 gacccgcgtg tccagttgct caccgcaacc tacaacgacg tgctcggttg cgaggctcga
     1441 cccgtggctg tcggcgacgg cacccacgcc cgctttatcc cgcgcgcact caacttcggt
     1501 cccgaattcc atgccgacca tgtgccggcg gtcgacggca tcgacctgga gaccccgcca
     1561 ttcatcgcac caggtgccgg caacgcgcac ggtgccgatg aatgggcctc gctgaaaaac
     1621 ctgaaaacag cgtttgtcct atatgcgtta ggcctagtgc gtctcgacca atacctgcaa
     1681 tagctggcgg ataagacgtt tgacgtgatt atgaccttgc gtatgctttt tgacgagcct
     1741 actattcgta ttgttcaagt acgccatgtc ggggccgacg cgtctggtga acacgccggg
     1801 tcatacacgg atgatagagt gaaattccat ggttagaaga acacatggta attctcaaga
     1861 acacgtcacc aagcatattt tcgtcactgg cggcgttgtc tcctctctcg gcaagggcct
     1921 gaccgcatct tcgctcggtc gtttgctgcg cagccgcggt atccatgttc tgcagcaaaa
     1981 gctcgatccg tacatcaacg gcgacccggg caccatgaac ccgttccagc atggtgaggt
     2041 ctacgtgacc gaagacggtg ccgaaaacga tctcgacatc ggccactacg agcgtttcct
     2101 cgacgtcttc ctgagccaga aggccaacgt caccaccggt cagatctatc aggaagtgct
     2161 gcgcaaggag cgcgccggcg aatacctcgg ccagtgcgtg caggttatcc cgcacatcac
     2221 caacgaaatc aagtctcgta tgcgcgccca ggcttccga
//
LOCUS       DAWBMW010000230         2256 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_9183_length_2256_cov_3.125852, whole genome shotgun sequence.
ACCESSION   DAWBMW010000230 DAWBMW010000000
VERSION     DAWBMW010000230.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9183_length_2256_cov_3.125852"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          429 a          677 c          663 g          487 t
ORIGIN      
        1 ccgtcagccc gctgggcttt gcggggggta caggttgcgt tgattgtaca ggttgcgttg
       61 attgtgccgc taagggtgac gaagacttgg tctccggcct tgcggcatcc gtctgatccg
      121 tcgccgcagc cggcttgcgc aaacttgccg catccgcttt gccgtccaat accaattgca
      181 cgtctggagg cacatcggtg ccatgtgcgt attcggagcc cagcagcgac tgccagcctt
      241 caatcggtag atagccgtcg cgcgcacccg aacggcaatc gcccgcatag gtgcgtgcca
      301 gttcatcgac cagctcattg ccttcgttgc cggcgtgacc tttgacccat cggaaatcca
      361 ccgggccagg gcgttgtgag atttcgcggt cgatcgcctg aataagctct ttgttcttca
      421 caggcttttt ctgcgaattt ttccagccat tcttcttcca gcctttgacc cacttggtcg
      481 agcaattgat ggcgtactgc gaatccgatt cgatgagcaa tggctcaggg cccgggtgcg
      541 ctcgcaaagc ttccagcact gcgcacagct caccaatctg attggtgccg ttggtcgcgc
      601 cgcccgcatc ataaccatgg tcgtcgtgct tatggccggg cttgccgccg gcgttctgct
      661 catgatcggc ccaggcccag cccattggcc cgtttggatt ccctaatgcg gacccatctg
      721 tggatactgt gatagtcatg ttctccaccc taacgcaagc tgtgcaatct cttgctgaag
      781 tggggttgcc cagtgcgagg caaaacaacg tagtgaagag tgtgaataac atcacaccta
      841 ttgactttgc ttgaaaaaca tgccatcgcg cgtgaatagg tgagcgctca caagaaaaac
      901 aagacatttc ctctctgcgg gtgaggccgg caacaatggc gcatccggta tctggaatga
      961 gaataaggga ctgtacaaaa ccagtgacgg caacatcgaa atcctcaaac cagaggcgga
     1021 cagcgatggt tcgacctcca cggccgcatc caagggcacc atgcttatcg caggcaactc
     1081 caaaaccgtg gcactcggtc caggcggcat ggccttggct cccggcctgc cgctcggtct
     1141 tggctggttc atccgacgcc gcttaggatt ggatgcctcc gacattctgg accgtaagct
     1201 cggcttgaag taacggctgg ttcggacgtg attgctgtca cgtacggatc atgacgacag
     1261 cgcgaaggtc ctcatcggtt tacgtcgatg aggaccttcg cgcgcattag gggagtctgt
     1321 tgcgggctgc gctacgcggg tcatgctgcg cgggcgtgct atgcgaacaa actacgcgaa
     1381 caagtcgttc agtgcctcgg caatcgtcgg atgcgtgaac accatatcgc gcaacgtgct
     1441 ggccggcgcg tcaagatcca tcgccaactt gacgatattg atcacctcgt gcgactcgac
     1501 ggaaagcagc atcgcaccca gaatgcggcc agtattgcgt tccacaatcg ccttcatcag
     1561 accatccggt cgccgcatta cctgggcctt cggcacagcg gccaccggca atcgcttgac
     1621 cacataatcc aatccagccg ccttcgcctc gcgctcattc aatcccacgc gcgagtaagg
     1681 cgtggcgagg aatgtgctcg acggcacatg cttgcggtcc ttcaccgtgt acggacggtc
     1741 ggaaccattg agctgcgacc acaccacgcg atagtcatcc agcgaaatgt acgtgtgctg
     1801 cgggccgccg ttcacatcgc ccagagccca aatgtcggct gctgtggtgc gcagaagctc
     1861 gtcgactttc acggcaccgc gttcggtgag ctccacgcca gctgcctcaa gatgcagtcc
     1921 ctcgacgttc ggcgtacgtc cggtggccac cagtaccgcg tcggtggtca ggcagaatcg
     1981 ggcttcagcc ggctcggcga accgcggctc gccatcgtct gatggtttcg tttgaacgtg
     2041 tgacatcgtg gcgtgatcgg tcgggttgct agtggggctg gtggttgttg tagtgtcggt
     2101 ggcgccggtg gcgccggtgg caccggtgat atcggtatga gcgttcccgg tgccggcggg
     2161 ggtggaatcg caagcttgcg atgcgccgcg agtcataccc ttcaccgcaa cggacaacct
     2221 gacgccgccg tcagcagcag gcgcgatggc cttggt
//
LOCUS       DAWBMW010000231         2178 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_9575_length_2178_cov_4.773434, whole genome shotgun sequence.
ACCESSION   DAWBMW010000231 DAWBMW010000000
VERSION     DAWBMW010000231.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9575_length_2178_cov_4.773434"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          491 a          639 c          628 g          420 t
ORIGIN      
        1 ctggcttggc tcactcgttg cactactact ttcataaccc caccttaccg cgcgatcagt
       61 ttcgccttgg tatcggccgg cttcagtaga acttttcgcc gttgagctgc tggcccacca
      121 aaaccattca gcagatcgtc cctgcctcat gtttgtcagt gtgtgctatg cgttgatgcc
      181 gcgcgtcgca cagcgaccga agccagcaaa ccgatgagca gcatgcagcc tgccaccaaa
      241 agtgtccaac ggttgccctc aatgaacccg gaagtcatcg catcggcgat tttcggcgct
      301 tcgtcaccga attgggccgc cgatccatct ccgctgcgga acgagccaat tacaccgcca
      361 gccgagctgc tgaccgcctt ggccagtcca tcgccgactt tagcgggcag tccaagggat
      421 tcaagtcgtg ccggcaacgt cccattgatg gccatggtca atgccgcgcc tgagatggcc
      481 gagcccatgg cggtgccaag ctgacggacg gtggactggg tggccgatcc ttcaccggac
      541 tggggaatcg gcacttcgga cagcaccacg ctggtcagct gcgcggaagc gagccctaag
      601 ccgcagccgt aggcggtcaa aggaagaagc tgccaccaca ctgcgaattc caccggcatg
      661 aggactgcaa tcacggctac cgcgatgatc tcaatcacca agccaagttg gaccacgccg
      721 accggagtga atttggaggc cagcatatgt gcctgactac cggcgataat agagcccaat
      781 cccatcacgg ccagcatcgc accggcctgt agcgtgtcga gtccgcgcgc gttgatgaga
      841 tacagcggaa gcgcgaacac cagcgcgaac tcgccggcgg cgatggccgc agccgacaga
      901 ttgccccacg agaatgtctt gatgcggaac agggtcatgt ccagcatcac cggtcgcccg
      961 gctttgcctc gtgcaagttc tacggttatg aagacgacca gcaggagtat accggcgacc
     1021 agacaaatcg gcaccggcga cagccagccg tggttccatg tcagactacc catcttcaac
     1081 tgagtggaag tctgcttcca ccagccgtag gtctcgcctt cgataagtcc gaatacgagc
     1141 agggcagaag tcagtgcgga cagcaccacg cctggaatgt ccacatcaat gctttggaat
     1201 cgaatggtag aagaagcggt tgaggaagtg atgttcgagg tatggccacc ggtctttggc
     1261 accaaaggaa tggttgccac aaaaatgatg atgcctagcg gaaggttcac caggaaaatc
     1321 catcgccacc ccaaggtctc ggtaagcacg ccgccgagca acggtccgag agcggcggct
     1381 gaactcatta ccgcgcccca gactccgaaa gccgcagcgc gatacttgcc acggaacgtg
     1441 gcggacaccg tggacagggt gttcggcatg atcatcgcgc caccgatgcc ctgcaacgcg
     1501 cgagcggtga gcagcatgcc agcgtcctgc gatgtggcgg cgagcaatga acttgccacg
     1561 aaaatcacag ttccaatttg gaataccagt ttgcgtcctt tggcgtcgcc aagcttgccg
     1621 aagggaagca gcagcgcggc gagtacgatg ttgtacagtg atgtgaccca ctgggcgtcg
     1681 gtcaggtcga tgccgatatc gtcgataatg gtggggatgg agacgttgac gattgagctg
     1741 tccagcacaa tcatggccag tcccaaagcc aacacccata gcgcgcccca tggcggagtt
     1801 tgttccggat gaggtgacgt ggacatcgtg gaagacgatg gcatatcggg ctgaagctga
     1861 tgttggaact tagatggctg ttccatgatg ccgtttcctc tcatagtcat aatgcagggt
     1921 ctgtcatgtt tgactgccgc catactacac gcatggcgtt gcgcgctcac tcatccaaac
     1981 gattcagcct tgatgtttag ccatgggagt aatccatgac aggcagagac ttaggtgacg
     2041 tgcccgtcac ggaaccaacg gaacccggct atcgggattc catagtcccg gttcttgcat
     2101 cacaatctgg aggcggcacc tttgtcgata agccagagca gttcctcacc atcggcgtag
     2161 gagcttggcg catgcgga
//
LOCUS       DAWBMW010000232         2170 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_9620_length_2170_cov_2.294563, whole genome shotgun sequence.
ACCESSION   DAWBMW010000232 DAWBMW010000000
VERSION     DAWBMW010000232.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9620_length_2170_cov_2.294563"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1685..1694
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          437 a          623 c          674 g          426 t
ORIGIN      
        1 agacttgtac tgggtgagcc ccacgacctg acggtacccg gagttcacca ggttgctcaa
       61 cgggaaatcg atcagacgat acacaccacc gaagggaacg gcgggcttcg cgcgatcacg
      121 ggttaacggc atcaggcggg tcccctcacc acctgcaagc acaatcgaca gaatcttggg
      181 attcttggcc atcgtaactg tcctctctgt tactgagcgt ctttcgacac cgcactgctg
      241 ggccggatgc ttccttgcat tcggtttaca tagttaataa ttgcagaaaa attcgaacat
      301 gcaaggtttt cgcccccgcg tgccgtgaac ttgcgatgaa cagtttcgta gatttgttaa
      361 ggatccggcc ttttcggagc gcgtgcgatt ccttagcgtg tcgcgtagaa cgccactgcg
      421 ctggatgccg ccacgttcag gctatccacg ccgtggctca tcggaatctt cacggtcaaa
      481 tcggcgcggg aaatcgtatg gtgggacagg ccgtcgccct cggtaccgaa gatcagcgcg
      541 agcttgtcga tatgcgtcgg atcttcggca ggcgcgtgca gacggcgtgt cagttcgtcc
      601 aacgaaatcg aacgatcctc caatgccatg gccacggtgg tgaagcccag cgacttcaac
      661 tcgtcgatgc cctggaacgg ccagtaatgc ttgttttcgg tgccgtcgtc gttgaacccg
      721 gtgatacggg tccacggaat ctggaatacg gtgcccatcg acacgcgggc ggcgcgtcgg
      781 tacagcggat caccgcagga gggtgtgacc agcacggcat ccacatcgag cgcggccgcc
      841 gaacgcatca gtgcgcccac attggtgtgg tccacgatgt tttccattac tgccacgcgg
      901 cgggcgctct tgcagacctc ggctacggag ggcaacggcc agcgtcgcat cgcgcatagg
      961 gcgccgcgat gcaaacggta gccggtcaga cgcttgagct gctcaggtga ggccacgtag
     1021 acgggaatat ccgcgcccca gcgcccgtcg atgaagtcga aggtgtcttt catgccctcc
     1081 agccaaggtt cctcgaccag cagtgacagc ggttcgcggt cagcggccag cgcgcggtcg
     1141 ataaccttgg gggactcggc gatgaacacg cccttggctg gttccaagcg attacgcaac
     1201 tgcatttcgg tgaggctcac atacgcttcg acgcgcgggt cgtcgacggt gtccagcgtg
     1261 atgaactgca ttggtggcct ttcgtgtgtg cagataacga aaaacccctt gcaatgcaag
     1321 gggtttatct gtgcccgaga cgggacttga acccgtacgc ccgtataaaa taggcactag
     1381 cacctcaagc tagcgcgtct accattccgc cacccgggca ggtgtcgttt ggacaacgag
     1441 tagatagctt agcatgaaat tacagggatg caattcgcgc gtgtgcccgg cgtgtcgcgt
     1501 gtagccttga agtcatgacc gatgcccttt ttcttttcga cccgcaggtc gacgacgtcc
     1561 cggtgaacag cgacgaactc cacgccggct ggaagctaac cctgcccgct cacatcaagc
     1621 gccatgccgt gcaggccatg cgactcaagg caggggacag cctgcaactt tccgacggca
     1681 acggnnnnnn nnnnctgaag tcggggaagt ggggcgcgac cccgagccgc tcacccgttt
     1741 ggcgctgatt caggcattgg caaaaaccgg ccatgatgag caggccatcg atatggctac
     1801 tcaaatcggc gtcgaccaag tggtgccatg gcaggcggac cgatccattg ccaagtggaa
     1861 ggtcggccgt acagacaaga agtggaattc ggtactggat gccgccaccg agcagtcgcg
     1921 gcgcgccttt aagccgcaac tggaggcgtg cgcgtccagc aaggaggtcg tggccatctg
     1981 ccgcagggcc tgcgtgcatg gcgatgtggt gattgtgctg catcaggatg ctaccgatac
     2041 gtggtccggc gtggaggaga aggttgcgca gttggtggaa cgcacgctcc aggacggccg
     2101 gccgcggact gtcagtgtgg tggtcgggcc tgagggcggc atcagtgagc aggaggtagc
     2161 tgatttcgtc
//
LOCUS       DAWBMW010000233         2156 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_9711_length_2156_cov_2.075678, whole genome shotgun sequence.
ACCESSION   DAWBMW010000233 DAWBMW010000000
VERSION     DAWBMW010000233.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9711_length_2156_cov_2.075678"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          436 a          604 c          651 g          465 t
ORIGIN      
        1 accatcaaca caccggtctc cgacaaggag ctcgccgctc tgaagcgctc tgccgagacg
       61 ctgaaggaaa ccgccgccca gttcggcttc tgataaaaaa tcgctgtacg gagcgccttt
      121 cgcaccgcac aagactcgac ctacctttgt aggccttcaa cttgtgcggc acgaaaatcg
      181 cacacgtaca gcgatttttt ataccctgct gaatgctccc gttgggagca ttttttattc
      241 gtggatttcg ttggaggagg gggagctata gccctcgggc tcgagctgga aggtggtgtg
      301 ggggaccgag accgggaagt gctcgcgcag gcaatcctgc agttgggtga ggattgtggc
      361 ggcatcttcc atggtgaggt ctttgtcaac taccacgtgg gccatcagaa tcggcatgcc
      421 ggtggacacc gtgctggcgt ggaggtcatg cacggcaagc acatgcggca cgtcttccag
      481 atgttcgcgt accttatcga ggtcaagtcc atccggtgtt tcctccagca gcaccttgac
      541 cgcgttgtgc agcaacttga tggcgcgcgg aatcatcatc agtgcgatga cggcaccggc
      601 cacggcgtcg aagccattcc atccggtgga gatcattacc agggcagagg ccaccacggc
      661 caccgagcca agtgcgtcat tcatcacctc gaggaacgcg gccttcatat tcatgttgtc
      721 ttcgcgctgc gaggcaagga tgaaaatcga gatgatattt gcggcaagac ccagaatgcc
      781 cacgaacagc agcaagccga tatcgtcgat ttccgctttg gaaccgccga acagacgcat
      841 gccggcctcc atcaacgcat agatgccaac gacgagcagc accacggcac ctgcggcagc
      901 ggtgagcacc tcaagcctgg cccagcccca agtacgagtg ttgctgggct tgcggcgcat
      961 cagtatggcg gtgattgtgg atgccgccag cacggacatg tcggtgagca tatgaccggc
     1021 atccaccagc agggccaatg agccggttaa gacagccgac accacttcga tgaggaagac
     1081 cgaaccggtt acggtcaacg tggcaatcag cctacgctgg tgcgcggcag ggtttacgga
     1141 tgtggatggt gcgggtgcat gggtatgagc catatgtact ccttcgctct atcgagttga
     1201 aaccgcttct catttccatt tgcgatatgg tctgaatacg acgaaacccc ggcgcgaggc
     1261 cggggtttcg taagctgtgc gtgactatag cacgaccagc gcgattcggt gcacgctgat
     1321 cagctggtgg cgaacaggct acaatttgcg cagcacggtg accaccttgc ccataatcgt
     1381 ggcatgtgtg ccgtcgatgg gcgagtatgc ggggttgtgc ggcatcagcc agacatggcc
     1441 gttttccttg cggaaggtct tcaccgtggc ctcgtcgtcc agcagagcgg cgacgatatc
     1501 tccgttgacg gcggaattct gttcgcgcac caccacatag tcgccatcgc agatagcggc
     1561 atccaccatg gaatcgccgt ggacttccag catgaacagg gtgccggagc cggtcagccg
     1621 ttccggcaga cgcatgacgt cgtccacatg ctgttcggcg gtaattggca cgccggcggc
     1681 aatacggcca acgagcggca catcgtgtga ttcggcaatg gcttcggcgg ggaattggta
     1741 tatctccgcc acattactgg tgctggttgc ctcttcgctg gcttgggagg gcttttcggc
     1801 attcggggcg gatcctgcca ccacctcgat ggctcgaccc ttgttcgcgt tcatgcggat
     1861 gaagcccttg tcttccaaaa cttgtagctg atgcttgacc gaagacggac ttttgagccc
     1921 ggcggcattt ccgatttccc ggaaagaggg ggcgaagcct tgttcgtcga tatgcgtgcg
     1981 aatggcgtcc aacactttgc gctggcggtc ggtcagggtg gactcgtccg gcttctgctt
     2041 gggggagaag ggaatggtgc tcacgtcagg gctcctttcg ttacgtcacc agcataacgt
     2101 gttgttcgtg tgaaatcaaa catctgttcg agcttcgact tgaccgtgtc gaacgt
//
LOCUS       DAWBMW010000234         2135 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_9828_length_2135_cov_1.997115, whole genome shotgun sequence.
ACCESSION   DAWBMW010000234 DAWBMW010000000
VERSION     DAWBMW010000234.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9828_length_2135_cov_1.997115"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          435 a          647 c          565 g          488 t
ORIGIN      
        1 cccttccacc ggcttgccat cgaagtcggt cataccagtg gcgttttcgg ccgcaagtac
       61 attattcaaa gcatcatgcg cattgtcgcc aatggtgaaa tagtagtcac cggcgctcag
      121 aatatagccc ttggccttgg tgtagtcata gctggccaac aggtagcggt ccaccgtaac
      181 gtccacggtt tcggaagcac ccggctgcaa tgtcttggta cggccttagc cggcaagctg
      241 gatggcggat ttctcgacca gattcttccg ctcatattca ccatacgggg tctgcgcata
      301 cagttccact acagaggcac ccgcaatatc accggtgttc ttcaccgtta ccttggcggt
      361 gaattcatcc ttgtcacggt cgtagctcac accgtcgagc gtctgttcaa aagtggtgta
      421 gctcagaccg tacccaaacg gataactgac ttcgtccgca tagttccatg ccgaggcacc
      481 atgtgaagct ccgaccgagc tggccgctcc gctgcccgga ttcgtcacgg cgtccgcgta
      541 gcgggtctca tagtatttat agccgacata gatgttctcg ggctgtacgg tcacattatc
      601 gatattgacc ggcttatcgc tgactgcggc gttcacttca tcgacgttcg tccattggtt
      661 tgagttctgg ctggaagtgt gcgcggccgg cgacgacatg gaatcgacgg catacgtgtc
      721 ggtcaaacgg cccgaagggt tggccttgcc caccaacaga ttggccactc cttcgaaacc
      781 acgctgccct ggattgccga tccacatgca ggcatccaca ccgtagtcat caagccagcc
      841 gacctccatc ggaaaggcgg aattgagcag cacgatggtc ttggagaact tgccggaatc
      901 cttgatcatc cgcagcaggt ccttctcgtc ctgatgcaga ctcagagcac tgatgccctc
      961 cggatccttc atcatcatct ccttgtcttc accgccttca cgggcaagca tgacgatggc
     1021 gacatcgtgg taatcgtcct gccagctggc cttgagctga tcggtataga acgatgccgg
     1081 ctcctcgcca agcaccgggg cgtcattcaa cgcgccattc gaacgtggat cgccggaaac
     1141 ctgcaggttg ttgctggcca cacgcttggt atcgctcttc gaataggcgt caaacagagt
     1201 gttgttcacc gagaaaccgg catgttccag tgcggatttc agatcaatca catatttgcc
     1261 gatgtcggcg gcgctgttgg cgccacccgg gctgtagacc ggctgcacca cggcgtggcc
     1321 gaacaacgtg acatgtttgt ctgaggcaag cggcagcgtg ccgtcgttgt tgcgcaacag
     1381 caccgaaccc tcctcctgct caagcacgga ctcgccgtac gcgtcggcga tgagcttctg
     1441 tagattttcg gcattcaagt cgccatattc gctggcgtag tacgtagtgt ttccgccgcc
     1501 ttcaccggcg acgattctcg tgctggacgt tcccagcgcg gaggtgatcg ttcccgcata
     1561 catgtacgag cattgcgtcc cacagatgcc aagcaccagc aatgtgcctg tcaccgatgt
     1621 gagccctcgc cacagcagac cggaattccc tttcatctcg actctttcgt tgtcacatgt
     1681 tgataccgac atcaatgtca attccatggt gcgcggcacg gccaccccct accttcccgg
     1741 atggcgtaac tggtcattcc caggggcata actggttctt tcgccattcg acccgagcat
     1801 gtgacgtttc ctataatgga atccgtcgca atgaggaggc atgaaccccg tgaggatcgc
     1861 aatcatcgac gatgacgccg acgaacgcaa gaccctgcag gcatcgttcg aacggcttgc
     1921 gcaggagagc ggatccgcaa tcgtcatcat tgaatttgcc ggcgccgatg attttcttga
     1981 cggctacgac cgctccttcg acctgatctg catggatatc gacatgcccg gcaccgacgg
     2041 catgtccgcc gcacaacgcc tacgtcagat ggacgccgat gttccactgg tattcgtcac
     2101 caacatggcg cagatggcga ttcatggcta tgccg
//
LOCUS       DAWBMW010000235         2130 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_9856_length_2130_cov_2.258795, whole genome shotgun sequence.
ACCESSION   DAWBMW010000235 DAWBMW010000000
VERSION     DAWBMW010000235.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9856_length_2130_cov_2.258795"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          357 a          634 c          673 g          466 t
ORIGIN      
        1 cgcatgcgca ccgccaacgg ccgtgtcatc gactccggcg catcagccgc cccaaacaaa
       61 tcgttctcac tcataacgtc ctatagtacc ccagtgttcg aacagacgtt cgagctgcac
      121 cttggcttcc ctcagagagg ggagccagga atcagttact cacctcgtag tcgtggaaga
      181 ctacttcgcc ggtgtcttgg gtggcgtcaa ggtccacgat gccttcgatg gcccagtcgt
      241 ggtcgccatc ggaatcatcg ataatctgac gcaccttcca cgtatgctcg tcgttctcgt
      301 gcttgtcatc caacatgaac agttcgggcg agcgtgcttc ggcgccgatg cccacgtact
      361 cgtgctcgtc gtagtagtcg tcgagcacat cttcccattc gtgcacgccg tagccccagt
      421 ccttgtcgag cgcgccgagc ttgtccggct ggtcaaggtc cataagccgc acacggcgga
      481 acaaggcatt gcgcaccagc acggtaagac cacgacggtc ctcgaccacc gcgctttttg
      541 cgccgggcgc ggccagggat gccgcagcct cggattggtc agccgagtca ccggcgttct
      601 cccattcgtc gaccagcgag gagtcgatgg agcgcacgag cacgcgcagc cacgagatga
      661 tgtcttcgag ttgctcgtca cgcttttcgg gcggcacggt gcgcgccagc gtgcggtagg
      721 cgtccgagag gtagcgcaac agggtgcctt cggagcgggc gatgttgtat cgggtgatgt
      781 atccggtgaa gtcggaggcg gtttccacca tgtcgcgcac cacggatttc ggcgacagcc
      841 agtagtcgtt ggcccacggc acatcgtggc ggtactgatc gaacgcggct tcgagcatgt
      901 cttccagtgg cttcggatac gtaatctcct gcagcttatc catacgctcg tcatagtcga
      961 gtccgtcggc cttcatatcg gccatcgcct tgtctcgggc ctgacgctcc tgggcacgca
     1021 gcacctgctt ggggtcttcg agcgtggcct cggccatgga gatcacgtcc agcgcatagg
     1081 tgtcggactc ggggtccagc agttcgagcg cggccagcag gaacggggag agcggctggt
     1141 cgagggcgaa gtcgtccggc atatcaaggg tcatgtagta gtccttgccg ccgtccttgc
     1201 ggtcctcggt ttcgatgact tcggtatcga acagggtttg gaagatctcg tcggcccgct
     1261 ggtgcagatg ctccttttgg tcgggggtct gcgcggaatc gtcgatgaga tcgtcgatac
     1321 gggcacgggc gtcgccgccc tgcgccactt cgttgagcac catcgaatgc gtgattttca
     1381 ggtgcgggac cagggtttcc ggttcggcgt cgatgagctt gtcaaacgtg ctctggttcc
     1441 aggtcacaaa accttccgga gccttcttgc gcttgacctt cttgagcttc ttcgggtctc
     1501 ccccggcctt ggcgatggct ttctggtttt cgatttcata ttcgggagcc tcggcgatca
     1561 ccagtccctc ggtatcgaat cccatacgac cggctcgtcc ggcaatctgg tggaattcac
     1621 gggcgcgcag gcggcgcatc ttggtgccgt cgaacttggt cagggccgtg agcaccacgg
     1681 agtgaatcgg cacattgatg ccgacgccca atgtatcggt gccgcaaatc acgggcagca
     1741 gaccctgctg ggcgagctgc tcgaccaggc ggcggtagcg tggcagcatg ccggcatgat
     1801 ggatgccgac accggtgcgc agcagtcgct gcagaatctt gccgaaggct gtggtgaatt
     1861 tgacgccttt gatggcttct gcgatggctt ggcgctgttc cttgctggac acgccggtgg
     1921 aggcgagcgc ctgcgcggtc tccagcgcgg cgtcctgcga gaaatggacc acataaatcg
     1981 gggtttcgcc gttgcggaag gcgagttcca cggttttttc catcggatcg agcgtgtatt
     2041 cgtagcttaa cggcaccggt cgcggcgcgt cggcgattac atccacgtcc ggggtgtcgt
     2101 tcagatcgga gagcttgtcg gcaatggcgt
//
LOCUS       DAWBMW010000236         2123 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_9899_length_2123_cov_2.988395, whole genome shotgun sequence.
ACCESSION   DAWBMW010000236 DAWBMW010000000
VERSION     DAWBMW010000236.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9899_length_2123_cov_2.988395"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1804..1813
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          421 a          596 c          645 g          451 t
ORIGIN      
        1 ttgttgacct cttccaggcc ggcgagcata cgaagggtcg tagacttacc acagccagaa
       61 gggccgacga ggacgaggaa ctcgccatcc ttgatatcaa gattcagatc atccacggag
      121 ggcttatcgt tgcccgggta gatacgagtg acgtggtcga aaacgacttc tgccataatt
      181 actaatcctt tcagaggcag gtacgtgcct cacgatccgt tgtaaaggga tttgttcttt
      241 actgtgcttc cgccctcgcc accgacgtac aactttgctt tcggaaactg caagcttcag
      301 cgtttacttt tttccggttc agtgaaggtt ctcttcgttg agccgagaac tactatacac
      361 ggaccgtgga ccaaggcaag tggcgtgtct gaaatgtagt taactttatg ggcgtttctg
      421 ggggtgttgt gtggttcggc ccatgacacg cctgtttctc gaacgtgcga accggcacgc
      481 ccggcgtaga gcaggcctgc tcttcccgga ctgatggatt ccatcagtcg ccgactaatg
      541 cgacacagac tcgaaggtga cgccgttgcg ggcggctttg cgctcggcct tgcgctcctc
      601 ggcgtcttcg atgcgctgga tgcgcagctg gcgccagtcc tcgcgcatgt cgcggaaacg
      661 gtcggccagc gttacgtcat cgttctcggc atcgccatca gtactgctgc catcggcctt
      721 accgaacgtg ccatcggcca ccttggcgat gcccagcttg agcagtccga gcaactcacg
      781 caacgtttcc ggtacgggct catcgccctc atcggcggac ttggtcaggc cctcgatgcg
      841 gcgacgaagc gcgtcggcgt cctcgggatc gatcttgagc ccggcgatca gcgactggcg
      901 catggtctcg taatcgaggt tggcaaagaa attcagctgt tgcagcgtca tgaagccctt
      961 gagctcgggg aatcggtgga cgatgtattc ctcgcggtcg tcggcggcgc gggtccagtc
     1021 gcgggtgagc tgcggatccc agctggcggt aatcgacccc tcgcgcatgc ggtagtggta
     1081 cagcgccttg ggcagacgga ccacgcgcgt ggcctcgccg atcacgttgc agatacggga
     1141 gagatcctcg attttgcgac ccaccgggaa ggagaatccg gtgccctgat aggtggaggc
     1201 gggtgccagg aaggcccaga aatagccgtc aatctcagcc ttgacttgct ttttgatgac
     1261 ctcgaccggg gtcaggacct gcacctcggt gaagtcgttg tggcgatagt tggacagcag
     1321 cggcttgttg ttctcggaga tggtgtcgaa ctggaacatc acgaggtcgg cgtcatactc
     1381 gcgcatggcg ttcaggcagt cctccaccaa cgtgggtgcc acggtgtcgt cggaatcggc
     1441 gaagtatatg tacgcgccga tagcctcagc gataccggag ttgcgggcgt cggacaggcc
     1501 gccgttgggc ttgtgaatca cgcgtatgcg cgggtcgcgg gcggcccagg cgtcgcacat
     1561 ggccgggcaa ttgtcaggcg agccgtcgtc gaccagcaga atctccagat tggcgtacgt
     1621 ctgcgcggca acagacgcca cgcattcgtc gaggaacttc tcgaccttat ataccggaat
     1681 aatgatgctg accaacggat cctgttccat atctccagcg taacgccgcc gcacgaacgc
     1741 ggatggaatc cggccgatat cagggacatc aggataagat gcttggagaa gacgtgacag
     1801 ggannnnnnn nnnatctcaa tcagtaccgt agcgaaaccg gtacggaatt cacgatttcc
     1861 gtattcgacg atggtgagca aatcgtcgag aaatatacgc cgatgtacga cattctgttt
     1921 ctggacatcg agatgcagca catggacggc atggcggcgg cccggcgcat ccgcgagcgc
     1981 gacaacactg tggttatcgt gttcatcacc gccgccccgc agtacgcgat caacgggtac
     2041 gaagtgcagg cgctctccta tctgctcaag ccggtgccgt acttcgcatt ccgccaggag
     2101 ctgaaacggt cgatcgatat ggt
//
LOCUS       DAWBMW010000237         2115 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_9952_length_2115_cov_1.862136, whole genome shotgun sequence.
ACCESSION   DAWBMW010000237 DAWBMW010000000
VERSION     DAWBMW010000237.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9952_length_2115_cov_1.862136"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1842..1892
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          414 a          654 c          621 g          375 t
ORIGIN      
        1 gagtatcagg acaccaacca cgcccagtac gtgctcgtgc gcgagctggc cggcgtggac
       61 acgggggaga aggccatccc gggcgcgcct aacgccggca agtccggccc cgcctggatc
      121 accgtggtgg gcgattccga ccagtccatc tacgcgttcc gtggtgccga catccgcaac
      181 attcaggact tcgagcagga cttcccgaac gccaagacca tcatgctcga acagaactat
      241 cgttcgacgc agaccattct ggatgcggcc aacgcggtga tcagcaacaa cgagggccgc
      301 aagcccaaga aactgtggac cgcgctcggc aagggcgagc cgatcgtcgg ctacgcggcg
      361 gacaacgccc agcaggaggc ccagtgggtg gccaccgaaa tcgcgcgcct gcacgccgaa
      421 gcgggcatcg cctactccga catggcgatc atgtaccgcg ccaacgccca atcccgctcg
      481 ctcgaagagg cgctgatcaa cacgaatcag ccgtatcagc tcgtcggcgg caccaagttc
      541 tacgaacgtc gcgaaatcaa ggatgcgctg gcctatttgc aggcgctcgt caatccggac
      601 gacgatgtga atctacgccg tattctcaac gtgcccaagc gtgggcttgg cgaccgcgcc
      661 gaaggcgtgc tgctcgccta cgcgcgtgag catggcacca gcttcttcta cgcactcatg
      721 cacttggatg agatcgaggt gccgacacgc accgccacct cgctacgagc attccgcgat
      781 ctgatgggcg cgctctcgca gttcacgcgc gcgcacgact ccaagcccag cgagatcgtg
      841 gccgaagtat tggagaagtc gggcttaagg gccgaactcg agaaatccgt ggacccgcag
      901 gacgcctccc gtctggagaa cctctcccag ctgcagtcca ctgccgccga attcgaacag
      961 aacacgcctg acgccacgtt gtccgcattc ctggagacca ccgcgcttgt ggccgactcc
     1021 gaccaactac ccggcgaggc ggaggactcc ggcaaggtga cgcttatgac cttgcacacc
     1081 gccaagggcc tcgaataccc ggtggtgttc ctgaccggca tggaacaggg caccttcccg
     1141 cattcgcgtt cgatggaaga caccactgaa ctgcaggagg aacgccgact cgcctatgtg
     1201 ggcatcacgc gcgccaagca gcgactgtat gtcacgcgcg ccgccgtccg ctcccagtgg
     1261 ggccaggccg ccgatatgat gcccagccag ttcctcgacg agattccgga ttcgctgatt
     1321 gattggaaac gccgtgaagc cggcgtggag cgtatgcgcg ccagctggga gaccgatgga
     1381 ttcgccgacg atttgggcgg ctgggatgat gacgattttg gcggcgccgc gtttggcggg
     1441 tcgtcgacgt tcggctcgcg tggttcgtcc ggttcgggtt cgtcctatgg gtcgcggtcg
     1501 cggtatgggt cgtcgtatgg ctcgggttcc ggcccttcgt cgtatggttc ccgttcatcg
     1561 tcgtatggtt cccgctccgg ctcgtcctcc tatggttccc gctcccgttc ccgtcccggt
     1621 tcttcgtccg gttcatatgg tggcacccgt ggcggcaagg tcaccacgcg tcgtaccgca
     1681 ccgaaatccg attcgaccgg ctctgccgtg cctagctcga agctcaccaa ggacaatggc
     1741 ctgaatatcg ccgacttcgc cgtgggcgac atgatcagcc acgaccagta cggtctcggc
     1801 aaggtcaccg acgcacaaga caagggccgc aattcggtaa tnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncggctccg ccggcgtcaa acgcctcatg
     1921 ctccgcgtag cgccgattga gaagttgtag ataaattggc ccctctgacg aggggctgag
     1981 ttatatacca aataaattcg caaaaaacaa gacttgtgtt cttcatcaca gtgtgggcat
     2041 aatgcggagg ttgtgcctac aagccctggg aacaggggag aggaaaaagt agttatgaag
     2101 aacaagctgt ttgca
//
LOCUS       DAWBMW010000238         2076 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_10177_length_2076_cov_2.399802, whole genome shotgun sequence.
ACCESSION   DAWBMW010000238 DAWBMW010000000
VERSION     DAWBMW010000238.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10177_length_2076_cov_2.399802"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          427 a          667 c          633 g          349 t
ORIGIN      
        1 ctgctgcgcg ccatcttcgg cgagaagtcc cgcgaggtgc gtgacacctc cctgcgcgtg
       61 ccccacggcg agaccggtac cgtcatcggt gtcaaggaga tcacccgtga ggatgccgag
      121 gaggacggcg acgagctgcc caacggcgtc aaccagatga tccgcgtcta catcgcccag
      181 caccgtaaga tcacggtggg cgacaagctc tccggccgcc acggcaacaa gggctgcatc
      241 tcccgcatcc tgccggaaga ggacatgccg ttcctggctg acggtactcc ggtcgacatc
      301 atgctgaacc cgctgggtgt gccttcccga atgaaccttg gccaggtgct cgaactgcac
      361 ctcggctgga ttgctcactc cggttgggac atctctttgg atccgaatct ggaagccgag
      421 tggaagaagc tgattccgtc cggtgccgag aaggccgagc cgaacacccc ggtcgccacc
      481 ccggtgttcg acggtgtcaa gcctgaggtc ctgaagggcc tgctgtccac cacgctgccg
      541 aaccgcgacg gcgaccgtct ggtcggcccc gacggcaagg ccaccctgtt cgatggccgt
      601 accggtgagc cgtacaccaa gccgatttcc gttggctaca tgtacatgct gaagctgcac
      661 cacctggtcg acgacaagat tcatgctcgt tccaccggcc cgtactccat gatcacccag
      721 cagccgctcg gtggtaaggc ccagttcggc ggccagcgct tcggcgagat ggaggtgtgg
      781 gcccttgagg cctacggcgc cgcctacacg ctgcacgaga tgatgaccac caagtccgat
      841 gacgtcgacg gccgcgtgcg tgtctacggt gccatcgtca agggcgataa cctgccgccg
      901 gcaggcatcc ccgagtcgtt caaggtgctc ctgaaggaaa tgcagtccct gtcgctgaac
      961 gtcgaagtgc tcaacgccga aggcgtggcc atcgacatga aggatgagga tgacgatccg
     1021 gccagctccg ctgacgacct cggcttcaac atcggcgccc gccccgacgc ggccgccaag
     1081 gaagaccaga aggccgaaga gcccgaatac cagtgatccc tcgggctgca tgcggtcaat
     1141 acgccgcgtg tagctccgcc aacgtgaaat aaataacgta cagagacctt tccggtttgc
     1201 actacccctc agtcagcctt ggctgacagc tcccctgaca aggggagctt gggaaggtct
     1261 caagaacaga aacggattaa caagtgctgg acgtcaacgc atttgacaag attcgcatcg
     1321 gccttgccac cgccgatgac atccgtggtt ggagccacgg tgaggtcaag aagcctgaaa
     1381 ccatcaacta ccgtacgctg aagcctgaga aggacggtct gttcggcgaa cagatcttcg
     1441 gcccgacccg tgactgggag tgcgcctgcg gcaagtacaa gcgcgtgcgc ttcaagggca
     1501 tcgtctgcga gcgatgcggc gtcgaagtga cccgcagccg cgtgcgccgt gagcgcatgg
     1561 gccacatcga gctcgccgcc ccggtgacgc acatctggtt cttcaagggt gtgccttccc
     1621 gcctcggcta cctgctgaac gtcaccccga aggacctgga gcgcgtgatt tacttcgcct
     1681 cctacatggt caccgaggtg aacgaagacg agcgtcacaa cgatctgccc ggcctgcagg
     1741 acgagttcga ttccgagatc aagcgccttg agcagcgccg tgactccgac attgaagccc
     1801 gcgccaagaa ggtcgaagag gatctcgccg cccttgagga ggccggcgag gccaagggcc
     1861 cggcccgcac caagttgcgt aacggtgccg agcgtgatat ggctgccatc cgcacccgct
     1921 acaacgacca gatcgcccgt gtggacgccg tcttcgacaa gttcaagaag ctcaagcccg
     1981 gcgacatggt cgacgacgtg gacctgtggc gcgagatgca ggaccgctac ggcgattact
     2041 tcgaaggctg catgggcgcc gaagccatta agaagc
//
LOCUS       DAWBMW010000239         2067 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_10226_length_2067_cov_2.899602, whole genome shotgun sequence.
ACCESSION   DAWBMW010000239 DAWBMW010000000
VERSION     DAWBMW010000239.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10226_length_2067_cov_2.899602"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1645..1654
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          441 a          572 c          633 g          411 t
ORIGIN      
        1 gcattcgtgc ggccaagcgc aagggcgtcg aggattgggc cggcaagcat gcgccggatg
       61 tctggtgcat acaggaggtt cgcgcgcctc aggacgatgt cgattctatt ttcgatgaat
      121 ttggtttcga atacgccacc gccggcaaga tcgaggcacc ggccaacctc catgccatga
      181 acgaggtctg ccgggtgaaa ggccgcgcgg gagtcggact gctcaccgac ttggaagtgc
      241 ttgacaagcg atatgggctg ccaggcctga gcgaggatgt ggattcgggg cgttggatcg
      301 aaaccgatgt caaaacgcct gagggctacg cgatcaccgt ggcatccgta tatgtgcatg
      361 ctggcaacac tgacgatccg accaaaatgg aacagaagta ccgttttctg gacacgatgc
      421 tgaagcgcat gggcgcgttg cgggacgagg ctgcgcatgg cggcaaacag gccgtgctct
      481 gcggcgattt caacatcgcg cacacgccgc ttgatatcaa gaacgccaag gccaatgaga
      541 agcacgccgg atttctgccc gaagaacgtg cctacgtgga caaatggctg gacgaatacg
      601 agttcgtgga tgtgatgcgt tcgttggccg gcgacattca ggggccatac acctggtgga
      661 gccagcgagg gcgcgctttc gacaacaatg tcggctggag aatcgactac cagttcgcca
      721 cgcccgagtt ggcggagacc gcacgcggat tcgtcatcga caaggctccc acgtatgaca
      781 aacgctggtc cgatcacgcc ccactcacga tcacatacga ggtatgacat attccgtggg
      841 acaacatccg aaaatcgacg attttcgcgg gccgtcccac gccccacagc atgagtaatt
      901 atcgaccaca ctaagaatgg cgtaattccg ggcttcgtga gaaatctgct gtgggacaac
      961 actcatgtct atcgattgat gttgatgttg tcccacaaat tgtgggacaa catcgggaaa
     1021 acaccaattt ttgcgttttc gtcccacgcg aaccatcatg caaacctgca catacgaata
     1081 ttgggttctt tccacggatg gaaagaaccc aatacaatgc gctcaaacgc agattatcag
     1141 tccagaccga gttggagctt gccgccgggg atggcgtcga gcaggtctcg ggtgtactgc
     1201 ttctgcgggt ggtcgaagac ctcgtcggtg gtggcatgct ccacgagctt gccgtgctgc
     1261 atcaccacaa cctcgtcggc gatctgacga accacggcca gatcgtgggt gatgaacagg
     1321 tagctcaggc ccttttcggc ctgcagatcg ttgagcaggc gcaacacctg gtcctgcacc
     1381 agcacgtcca aagcggacac ggcctcatcg cacacgatga cgtccgggtc cagagccatg
     1441 gctcgtgcga tggcgatgcg ctggcgctgg ccaccagaga gctcgttcgg gtaacggccc
     1501 atcacggaag ccggcatctc gaccatgtcc agcagttcct tgacacggtt ggcacgccac
     1561 ttcttgtcgc cgatcttgtg gatgcgcagc ggctcctcaa tggaacggaa gatggagtac
     1621 atcgggtcga gcgaaccgta ggggnnnnnn nnnntgtcgc gaccttcata gaacactttg
     1681 ccggaggtgg gcttcaggag gtgaagcacc atgttcgcca cggtggactt gccggaaccg
     1741 gattcgccca caatggccag tgtggtgcca cgcttgacgg agaaggacac gtcatcgacg
     1801 gccttgaaca tctccttctt gcggggcagc ttgaattcct tggtcaggtg gtcgacggtg
     1861 atgatgtgct cgctcttttc gagcgtgctt tcgccggcga tgtggtgatc gagcagagcg
     1921 tccgcatcct caccacgctc cttggcggag atgatgcgct gggaggccag cgacggggca
     1981 gcggccacca gtcgcttggt gtacgggtgc tgcgggtgct ggagcacttc aagggatggg
     2041 ccggattcga cgacctgacc cttgtac
//
LOCUS       DAWBMW010000240         2061 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_10270_length_2061_cov_1.876371, whole genome shotgun sequence.
ACCESSION   DAWBMW010000240 DAWBMW010000000
VERSION     DAWBMW010000240.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10270_length_2061_cov_1.876371"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    688..697
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1645..1654
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          395 a          626 c          624 g          396 t
ORIGIN      
        1 cgcaggaact ggtagcgttc accgttgcgc tggtattcga tgtccatgtt ttgctggacc
       61 gcgtcggcga tgccgtactt gtcgatctgc acagagtggt cgatgaccat atcggactgg
      121 acctgcgggt tgatgacctc cgggtcgccg ccgaggtcct tgaccgcgtc acgcatggtg
      181 gcgaggtcca cgatgcaggg cacgccggtg aagtcctgca tgatcacgcg ggacggcgtg
      241 aactggattt cgtggctggg ttcggcgttc ggatcccagt cgagcagggt cttgacgtgc
      301 tcgtcggtga tgttggcacc gtcgatgttg cgtaccagat tctcgaccag taccttcagc
      361 gagtacggca gatgatcgat gccgtcgaga ttggcgatgt tgtagtaatc gaagtccttg
      421 ccggctgcct tgagggtagc cagctggtcg tcgcgtaagg acatgcttcc tccgaaatca
      481 tgaatcggct tatgatgccg ttgattatcc gatacaaatg gtacgggtac gttactttgg
      541 ctctggcgtg tcggcttgaa gtgtgagaca attcgccgct attagatatt gaaaccgtgc
      601 tggcgcagta gattgccgaa tattctgccg accagcggag ccagccatag acagatcacg
      661 aaaatcatgg ccgcgccgcc caacgcannn nnnnnnnccg atgatgccgg ccgccgcgaa
      721 ccccgcgacc agcgcacctc gccagctgtt gagcggcctc gccacacggg ccagcacaaa
      781 cacgcccatc aaaaacagaa taatggcgct ggtggcgcgc aaagccgaca gatcggcgtc
      841 attgttcacc acatcccagc ccatgacgtt cggcagggtc catgaagcca gcaacaccga
      901 cagcgcggtg gcgatgccgc ccggcagcgc gaacgtgacc acgcgcttga ggaatcccgg
      961 aatatagcgg cgcgtgttcg gcgcgagcgc cagaatgaac gcgggcatgc cgatggtgag
     1021 agcgccgata tacgtgatat ggcgcggcag gtatgggtat gggatttgcg tcagcactac
     1081 gccgagcgag atcagcgcgg agtacacggt tttgacgagg aacaggctgg ccacgcgctc
     1141 catattggcc atgacctgac ggcctcgcgc cacgacgtcc ggcaggtgcg agaacttcga
     1201 atccaccagc accacctggg ccacggcctt ggtggcgggt gccgcactgc ccatggcgat
     1261 gccaaggtcc gcctccttga tggcgagcgc gtcgttgacg ccgtcgccgg tcatggccac
     1321 cacgtggttc tgcgtgtgca gcgcttgcac tatggccttc ttctggtcgg gcagcacgcg
     1381 gccgagcaca tcgacgtttt cgagcacgcg ggcaagttcg ttcacgtcct caggcagttc
     1441 gcgcgcgtcc atcgccaccg gctcatggtc gccggtgagt ttcactctgc gggcgatggc
     1501 acccacggtc actggattgt cgccggaaat cacacgacag cgcacaccct gctcacggaa
     1561 ccaagcgagc gtccgctcgg cgtcctcgcg aatcttctca gagcacaaca ccaacgccac
     1621 cggctcagcc tgcacatcca gttgnnnnnn nnnncttttg gcgacagctc ccctcggaga
     1681 ggggagctgt agaccgcgca accagcaaca cacggttgcc gtcattggca tactcgttaa
     1741 cctgctgcaa caccgacgaa tagtcaccct caagcgcaga aataatcacc tccggcgctc
     1801 ccatgtacca ggtctcgccg gatttacgca ccgcactcca tttgcgcgcc gaagagaacg
     1861 ggatacgaga ctcaaccgca cctgccccat atccttgggc cttaagcccg gcgagtacgg
     1921 cttggccggt gccgttgggc tgctcttcgt tgcagcagtc gtagagggcc tgcgtggctg
     1981 cctgttccgc agccgcattg gcggaatcga gcatcacgag ccggttgaag gcgatgccgc
     2041 catcggtgat cgtgccggtt t
//
LOCUS       DAWBMW010000241         2040 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_10394_length_2040_cov_1.876071, whole genome shotgun sequence.
ACCESSION   DAWBMW010000241 DAWBMW010000000
VERSION     DAWBMW010000241.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10394_length_2040_cov_1.876071"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          452 a          673 c          520 g          395 t
ORIGIN      
        1 atgaccggcg cccccgatca ggccggcaac tggcgcgtcg cccagctgcc gcagtgggag
       61 gaaggcaagg aagtctccgc cgaagacggt ggctctgcac ttgccgtcac ctcgcagtcc
      121 aagaaccagg ccgctgccta caagctcgtt gagtacatga ctcatggcga gggcgctcag
      181 accatggccg acaccggtac cttcccgtcc ctgaagaaga tcctccggtc cgactccttc
      241 accgacccga ccaccgagtc caacaagaag accaacgact acttcggcgg tcagaacgtc
      301 aacgaagtcc tttccgccgc ggctcagcgc ccgaccgaga agttccagta cctgccgtac
      361 aacccgtacg cccagaccgc ttacggcgac gaagtctcca aggcctactc cggcgacatc
      421 accctggaga aggcgcttga aaactatgcc aacaagctcg ccgagcaagg caagcagcag
      481 ggctacaacg tcaccgtcaa gtgacgcaac cgatctattg ccggtacatc cggcactgac
      541 caacaatcaa aacctgcggc taggccactc tccttccgcc tagccgcccg atctcccaaa
      601 gcggggatgc tccccagtgt ccccgctttc ccgtgccctc gcctgacgtg accgctccaa
      661 tcgagcgacc ccctggtagc gggcgcagca cagcaatccg tattccaaga aagcaatcga
      721 ttatgtccga tcaaacaatg gccgcagccc cgcagccaac ggtgggaatc ctgcctgagg
      781 aggcggatct gcacacggcc gaggcccgcg tcaacaagca caaggccgac tggcgcggct
      841 ggaagttcat gtggccgttc gccgtggtgt tcgtcttcgt gttcatcatc ccaattgtgt
      901 acgccattta catctccttc ttcaagaagc agatggtcgg cggcaccaag ttcgtcggct
      961 tcgagaacta cgcccgactg attcaggatc cgcagttctg gagctcggtg ggccgcgtca
     1021 ctatcttcac cgtattccag gtgccgatca tgctgtttct ggctgcagcc atggccctcg
     1081 ctttggattc gatgaagctg catggcacca agttcttccg catctccaca ttcctgccct
     1141 atgcggtgcc cgcagtggtc tccaccatga tttggggttt cgtgtatggc gccaaatacg
     1201 gtctggtcgg ctccctcaac gacttcctcg gcaccaacat cgatgtgctc aaccccgacg
     1261 tgctgcttgc atccatcgcg aacatcaaca cgtgggaata tgccggctac aacatgctga
     1321 ttttttacag ctccctaagc accattccgc acagcttgta tgaggccgcc tccatcgacg
     1381 gcgcttcgga gtggcagatt gttaagaaga tcaagctgcc ggagctgaag ggctcgctgg
     1441 ccatcacggt gatcttctcg atcatcggca gcttccagct gttcaacgag ccgagcattt
     1501 tgcagaacat ggttcctggc aacgccatca ccacgtacta cacgccgaac atgtacgcct
     1561 acaacctgag cttcaccggc aatcagagca actatgccgc agctctggcc atcgtgatgg
     1621 ccatcatcac catggccatt gcgtatgacg tccagcttaa gagtatgaag gagcagatga
     1681 agtaattatg agcactgcaa ccatgacttc aacaacaacc atgaccctgg ccgatctcaa
     1741 ggctcaggaa aaggccgcca agaaggccga gaaggagcgt aagaagcgca tcgacgccga
     1801 cgagaaggcc gagcgcaagc gcgccgccaa aaccggtttc tccaacccgg cgaacccgac
     1861 ccgctcgatg gccctgacca tcgtgtgcgc gattttcgcg gtctactgcc tcttcccgtt
     1921 tgtctacctg atgattaacg ccaccaagac ccagtccgac ttcacttcga cgttcggcct
     1981 cggtttcggc aaaacgttcg ccctgttcga caacatcgcc acggtgttca cctatcagga
//
LOCUS       DAWBMW010000242         2007 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_10598_length_2007_cov_2.423156, whole genome shotgun sequence.
ACCESSION   DAWBMW010000242 DAWBMW010000000
VERSION     DAWBMW010000242.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10598_length_2007_cov_2.423156"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1244..1258
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          408 a          660 c          566 g          358 t
ORIGIN      
        1 atcccgacgt gcgcacacgt cttgaggaac gcgagaaaac cgagggggcc ggggctttgt
       61 tcgacgagct gcgggccaaa gaccccgagg ccgccgcgcg catggaccca cgcaacccac
      121 gccgcaccat ccgtgcgctg gaagtcatcg agcttaccgg caaaccgtat tcggccagtc
      181 tgccgcgcta ccggtacgtg attccgtccg tgcagatcgg actcgacctg gaccgcccgg
      241 atttggacca tcgcatcgat ttgcgcacca agcagatgta tgacgatggg ttcatcgaag
      301 aagtcgaacg gttgcgcccg catctgggtg ccaccgccgt acgcgcgctc ggctatcagc
      361 agatcatcga cctgctggac ggcatctggg atgtgaacga cgccttcgcg gacatcgcac
      421 agaaaaccaa gcgactcgcc cgcaaacaga tgggatggtt cggccgtgac ccgcgcatcc
      481 actggctgca ggccctgaac ccgaaactgg tcgacaacgc gatggccatt atcgcgcacg
      541 ccgacgccgg cgactacgac ccgatcgacg cccgagccga cgaatacacc cagcatcatc
      601 tgggcgatat cacggcgtag gaatcagcca aaggccgatg caatcacccg caatcagtgt
      661 gcaggcccct gattccagcc ggcgatcaag aacacacgcg tcggattcgc cgggcgtagt
      721 atcgcgtcga acatcgtctc cagtgcagac gtgtcgcccc gatatgccga gtgcttggcg
      781 gaacgatact cctcgcgcgt cactgagccc cagtcgagat cccagccggc gtcatgcgcc
      841 aagcgtgaac cgaatatacg cagcaccatc gcattgccgc cacggaacgg atgcagataa
      901 ccgagctcat cataaataga agcgaacgcg tgaacgaaca cgtcgcgatc caacgcatgc
      961 aggttgcgct tgtccgccaa ctccatcgcg atgttgtgcg cgccggtctc aatgagccca
     1021 gccgggaaga acgcctcagg attcgcggcc cgagcccggt cgttcgtcac ctcgcgtgtg
     1081 gtgtttgagg tacgcaactt gcccgcatcc tcaaacacgc cttcgaacag ccccttatga
     1141 atggcggtca gctcatccaa gtcgccagac ggattccacc gacgttccgc caacaatacg
     1201 gtacgtgcga acaccgcatc cgcgcgatcc gtggcatgat catnnnnnnn nnnnnnnngt
     1261 ctgctcgtcc gccacgtcga tgagatgggt cacgggctcc aagccacgtt cgtaggcaac
     1321 cgcggcgcgc atcgttgcct cgcccggcgt catcgcttcc agtgcgcagt tcttgagtgc
     1381 aaatgcgact gcgctcgcac gctctgcggg tgtcatgcaa cccatgataa ggcatattcg
     1441 cgccccagcg gggaatcgac tcagacgaac gcggcgaatc ggctgtcggg attcgtcaat
     1501 cgaccatatc gtcacggtgt cgtcaccaac catgcacccc cgtactagta gaatcagacc
     1561 cgatatggca cgaacagcat cctccaacag caccaaaccc cgtaaatcca aagacaacgg
     1621 gtctacccat gatggcgaaa ccaaggtcca gcccgagctt gacctcgaac ctgagccgtt
     1681 gtggaagaag atcctgctcg cgattccgcg cgcgttcggc tccggcgtac gcgccgtcac
     1741 cggcgtcggc gaatacgacc cagcctaccg tcgtgacggt ttgtgcttcc tgctgctcgt
     1801 gctcgccgta ctgttctgcg cgtccgaatg gttccgcgtc agcggtccat tcggccagct
     1861 gcttcacgcc atcgcggccg gtgtgcttgg tctgatgagc gtcgtgctgc cggtgttgct
     1921 cgccgccgtg gccttccgac tcatgcgcaa ttccggtaaa gggtccaata acccgcgtgt
     1981 ggtcaccggt tgggtgctgc tcatgtg
//
LOCUS       DAWBMW010000243         1991 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_10685_length_1991_cov_2.686467, whole genome shotgun sequence.
ACCESSION   DAWBMW010000243 DAWBMW010000000
VERSION     DAWBMW010000243.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10685_length_1991_cov_2.686467"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1775..1784
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          377 a          672 c          569 g          363 t
ORIGIN      
        1 gcgctcgtca aactgggtct ggtgccatac ggcgacatcg tgccgatgac cgacgcctgc
       61 ccgacgctga ccttcaacac catcggccgc caccagagcc gactggtccg cacgcgcgtg
      121 gcctccgacc tctccccgtg gctgtccaag acttcggtcg gcgacattca caccgtggcc
      181 atctcccacg gcgagggccg cttcgtggcc tccgacgagg tgctcgccca gctcagggcc
      241 aacggccaga tcgccacgca gtacgtggac gagaccggca cccccggcat ggacctggat
      301 gtgaacccga acggctcgct gctcgccatc gagggcatca ccagcccgga cggccgcgtg
      361 tttggcaaga tgggtcactc ggagcgttcc ggcaacggcc tgtacgtcaa cgtgcccggc
      421 aacaagtacc agccgatctt cgaggccggc gtggaatact tcaccgcctg ataatcggcg
      481 cgtatcgcgt ctgatacggc acacagggaa ggaactctcg ggttccttcc cttttttgtt
      541 catgccggtc atgggctaag gttggggtgt tcgcactacc gaatccgccg ctgaaaggac
      601 ctgtcgtcat gccgatcaac gaggcattgt tcgccgcgat gaaggccgcc agctacatca
      661 agcccaacgc gggcaagtcg tacaaattgc agcgcgtggc ggaagagctt atcgccaagc
      721 aggcacccga caatccgaaa tgccgtgtcg aggacgcctt cgcgcctatg gcggatggat
      781 atgccgtacc attgcgagtg ttcacgccgc tggtcgccta tggagcgccc ctgcccgaag
      841 ccctggagga atcttccgcc aatggcacgc ccgtggcctc ggcaatatcc gcgattctgc
      901 ccgccgtatt acccaaagtc ctacccaaaa tactcatcaa ggaaagcacg tcgagcggca
      961 acgccgacgg tatttccggc aatgccaaca cggaacttcc ctcagtcacg ccgcgtggca
     1021 cgatcctgtt tttccacggt ggcggttgga ccacaggcgg catcaacctg tacacgcagg
     1081 cttgcgcgca tatggccgta cgtctgcaac gccgtgtgat ctcagtcgag taccggctgg
     1141 ctcctgaata ccgcttccct actgctgttg aggattgcta tgaggtagcc cggcagctgt
     1201 ttgctgggga attgccgatt tctggggtgg gtggttctgt tgatgttgat cacccccagt
     1261 cggctgcgcc gacagccccc gccggcgggg gcgatatatc tgcgacaatc ccagcaccgg
     1321 atcccgacag catcgtgctg tttggcgaca gcgcgggcgg caatcttgcg gccgccgtct
     1381 cactcatggc ccgcgaccgc ggcgagttca tgccgcgcac gcagatgctg ctttacccgg
     1441 tagtcggcaa cgattacaat cccgagacca gcccgttcga atcggtgcgg accaacggca
     1501 ccgactacat cctcaccgca caggacatgg ccgactacat cgacatgtac cgttcgtccg
     1561 tggccgatct gaccaatccg tatttcgcgc cgctcaccgc gcacgacctg agcaatcagc
     1621 cgcgcacgct ggtgttgtcc gccgaatact gcccgctcag agacgaggac gaagcgtacg
     1681 cccgtcgcct gcaattggtg aacgacaacg tctcctgtta ccgaattcac gatggcatcc
     1741 acggctacct gctcaatacg tcggccgtgg gactnnnnnn nnnnccagct cccggcaccg
     1801 gcaccaccgc caacgcaccg gagggaggcg acgcgtggca agacgtactt ggtaccgact
     1861 agacaacatc ggcaagttct actcctccca ggctggccgc tcctcgcaga ccgtgttccg
     1921 ctattcggcc gagctggcgg acggcatcga tccggacatc ctgcagcacg cgctcgacca
     1981 tgccatcgcc c
//
LOCUS       DAWBMW010000244         1984 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_10732_length_1984_cov_3.598237, whole genome shotgun sequence.
ACCESSION   DAWBMW010000244 DAWBMW010000000
VERSION     DAWBMW010000244.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10732_length_1984_cov_3.598237"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          381 a          588 c          587 g          428 t
ORIGIN      
        1 gatgccatga cgatgggcag cctcgatagt catctggatc atgcgcagca cagctggtga
       61 atgcgggtcg gcaaagcgtt cgagactcgg gttctgacgg tcacaggcca gcgtgtattg
      121 ggtcaggtcg ttggtgccga tggagaagaa atcgacttcg gcggccagct cgtcggccat
      181 aatgaccgac gcaggcgtct caatcataac gccgatctgg atactctcgt tgaagtcgat
      241 gcgctcgccg cgcagctcgc ttttggcttc ttcaagaatc cgcttggcgt cgcgtacctc
      301 ctgcacagag gtgatcatgg gcagcataat ggcgatgttg ccgtatgctg aggcgcgcaa
      361 taatgcgcgt aactggactt tgaaaatctc cgggcgggtc aggcagatgc ggattgcccg
      421 gtaaccaagc gcgggattat cctcgtggcc cagattaaag tagtcgacct gtttgtccgc
      481 gccgatgtcg agcgtacgga tgatgaccgg acggtcgccc atctcctgtg cgacttgtcc
      541 gtatgcatcg aactgaaatt cctcggtcgg ccagtcctcg cgttccagat acaggaactc
      601 actgcggaac aagccgatgc cctcagcatc gttggccagc acggcggctg cgtcagaggg
      661 acggctgatg ttggcgtaca ggtggacccg ctgaccggac ttggtttcgg tgggcttgcc
      721 cttgagcttc cgcaggaggc ttaggtgctc ctcagcctcg gccttcctgc gccggtattc
      781 ggtcagcgtg gcctcgtccg ggtcgacgaa taccgcaccg ctggtgccgt cgattacggc
      841 aacgtgacca tcatcggccg ggtcaaaggc gtcgccgata cctgtgatgg cgggcaggcc
      901 catcgtgcgc gccagaatcg cggtgtgcga gttcgctgag cccttggcgg tcacgaggcc
      961 caaaacctta gcgcggtcga gctgcgcggt atcgctggga gcaagatctt cggcgaagat
     1021 gatgtggctt tcgccgttct gcgcgatccc gttctgcaca ataccgttct gcacagcatc
     1081 gccttgtaca ctgccatcct gcgccgtgct atcgcgtttg ccttgcagca cgttcatcac
     1141 gcggcgagaa acatccttga catcagcggc tcgagcctgc atggtcgagc tatccatcgc
     1201 agcgaacatg gcggcgaact gttcggcggc gctcttcacc gcgaattcgg cgttcacctt
     1261 gtcttgcgca atcaaacctt cgacgcattc cgcatagtcc gggtcctgga gcataagccg
     1321 atgggcgtcg aacagttcgg ccttgttttc gccgaactca gcggcgacgt tgtcacgcag
     1381 ttgcttgagc tgagcgacgg ccgtgtcacg ggccgcgcgg aatcgatcca tctcggcctg
     1441 cgggtcgtcg attaagcggc gttctacggc ataatctacg gcggcgagca cgcggaccgt
     1501 gccgatagca atgccagcgg atacgctgac gccctgtatc gttctcatta caggtgctcc
     1561 ttgaggaatc gctcgaactc tgcagcagcg acagcttcat cgttgccctg cacccgaacc
     1621 gtaacctcgt caccttgttg aacatcaagt cccataacac cgaagatacg cttggcatcc
     1681 acggattttc caccgtaggc aacggtgatg ctcgattcat attcctgcgc tttggcaacc
     1741 aaccggccag ccgggcgggc gtgcatgcct tcggggtcgg atacggtgaa cgtgaatatc
     1801 tgaggcatga cgttgctcct tacacgtccg gccgtcacgc gcgaagacgg cctttccgca
     1861 ccagctacgg actgatactg gaaaaaacaa aaaaccggaa tctttcgatt ccggttgtct
     1921 gtgcccccgc agggattcga accctggaca cgctgattaa gagtcagctg ctctaaccaa
     1981 ctga
//
LOCUS       DAWBMW010000245         1975 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_10793_length_1975_cov_1.852083, whole genome shotgun sequence.
ACCESSION   DAWBMW010000245 DAWBMW010000000
VERSION     DAWBMW010000245.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10793_length_1975_cov_1.852083"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          357 a          611 c          590 g          417 t
ORIGIN      
        1 caataacgtc tgacccgcat ccgtgccgaa gttcaacact ccgaacccgg attcgatgcg
       61 cgcgtccacc atgcgtgcga tggtcgtgcc catagccgaa tacgcgacca ccctgtaatg
      121 gaagtcggtg ttcaacgggg gaatggggtc cacggccaac tgctggtcca acaggttcga
      181 ggcgataagc acctccgaac cgtcaggcat gacacggatg acggatgcgc tgaccgtctc
      241 cgactggcct tcctccggca cgccgaacga cacgatgacc aacgccgcgt aatcattgtt
      301 cgactctatc gcggccatcg gctcggccgg ggccagccag tccacgtccc tcacgacgct
      361 cgtgctggat tccaagccgg aaccgccgcg caccacgagc gtgatggtca acgtcgaatt
      421 gttgttcggc agatactggc ttgcgccgat gctcaggctt cgcgtggaac cgtccatcgt
      481 cttccggtat ttctccacgc cgtccgactg gatgatgagc gtctgcgagc tgacgcccgt
      541 atcgtccgcc acggtccacg ccacggtgaa cggtgtcgcc gtaatggtgc cggaaggctt
      601 gttgatgctg atgttcggat atttcgcgac cgtgaaggtc acgtagttcg accatgcgcc
      661 ccagtcggcg tggataccct tggtgcgcac gcgaatccta tacgagccgc agcttttggg
      721 cgtgcgctga taactggtgt tcgtggtctg ctcttcgatg accgtaacgt ccgaggggtc
      781 ggtgacctcc acctgcgcgg cggattgggc ggaaccgtca ggatgattcg gtttccaagc
      841 gaccgtcatc ggctgattga caacatacgc gccgttctgc gtcgggttca gaatcgtcgg
      901 cgcggaaggg gccacggccg tctggatagt gttgctgtac gtccagtcgg agaagagcgt
      961 ggtcttggag ttgtcatcgc cgtagacagg tcttcccact aacgccgcgt actggacttg
     1021 gcccgcagga gctgcggtgt cggtccacgt gacgttctgg attccgttta tgtcgggaag
     1081 ccagccttcg gccgtcgcac cgggggtgcc tccggttatg tcggcccatt cgccgccgtt
     1141 caccctgcgc cgcagtctga tgccatacac atacgatttc gacgcatcca cggtcacgcg
     1201 cacggactgt tcggacagtt tcaccgcgtt caccgccacg ggggcggccg gcgtcgtgta
     1261 gatgtagccc gagtacacat ggtcggacac tccgccaggg ttctgggccg cgacacggaa
     1321 ctggtatcgg gcgttcgcct tcaaccccgt gtacgaatag ttcaaggcgt cccagttcaa
     1381 cgccttgacc agaccccacg cgccttgtgt gccgccgttc aagccgacgc actggtctgc
     1441 gtagatctgc ttccaatatt ttcgcgccgc attatcatag ttcgactgcc atgcggcctt
     1501 cacgcttgaa tcattgaccc gcgtccatga tacgttcttc ggcgggttcg gtttcgcata
     1561 cgtgatgccg ggaaccgtga ggttcacatg cgcttccgac cgtcccggca aaccatatgg
     1621 gatgttcagg aacgcgcggc aggagaacgt ctgcgcggac tcctgcttcg tgacggtcac
     1681 ttgctgggtg tgtaaatcca cgtcgccgtt gaaggaccgg tagccgaagt tcaccgtgtt
     1741 cgtgctcgtg ctcacgccat tgacccaagc gccaccggac acggcatcgg acgccaccca
     1801 gcgcgacggg tcggtgcgac ggtagatgat gtgcacgcct atgacggcct gtgtcgcgtt
     1861 ctgcgagacg atatcggctt gtacgcagca acgccagccg ccgccgatga tattgccggc
     1921 accttcaacc atgacaaacc tttcttgacg atgttaggaa acaggaggaa accgt
//
LOCUS       DAWBMW010000246         1965 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_10868_length_1965_cov_2.431937, whole genome shotgun sequence.
ACCESSION   DAWBMW010000246 DAWBMW010000000
VERSION     DAWBMW010000246.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10868_length_1965_cov_2.431937"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          311 a          585 c          596 g          473 t
ORIGIN      
        1 gacctccacg agctccttct gcggcttggc gtgcttatcg acaccgtcga agccggaaac
       61 ggacatatgt ctgcgggaag gcgtggtggg tttataggtt ttaatcgaca ttgttcaatt
      121 cctcccttac accatgccct cgaagatctc gatgttcttc gaatcagcgg acagtttcac
      181 aacggccttc ttgcgggtgg cggtcatgcc ggacggattg gcgccgacgc gcttctcctt
      241 ggcgtggatc gtcgtggtgg tgaccttgat gaccttcacg tcgaagatct cctcgatggc
      301 ctttttgatc tcgatcttgc cggcgtcctt ggcgccttca aacacgtact ttttgatgtc
      361 gaggtcttcc atggactgct cggtgatgat gggacggata atgacatcgt aagcggttct
      421 cattatgcgt acacctcctc gattttctgg agcgcagcct tgtcaacgac caggacgcgg
      481 tgcgtcagga tcatgtaggg gctgaggatg gtggcgatgg tgctgctcac gccgggaatg
      541 ttgcgagagc tcttgacgac cttctcgtca acctgctcgg tcacgagcag agccttgccg
      601 tcggcgccga tcttggtcag gaacgccttg acgggggcgg tcttgatctc ctcgacggcc
      661 atgccgtcca cgacgaggat ctcgccggca gcggccttgg cgctcagcgc gctcttgagg
      721 gccagtctct tgaccttctt gttgagcgtg tagctgtaat cacgcggctt cggcgcaaac
      781 gcgacgccgc cgtgggtcca caccgggctg cccttgtagc cggcgcgtgc acggccggtg
      841 cccttctggc gccagggctt cgcggtggtg taggaaacct cgcccttggt cagcgcactc
      901 tgcgtgccct gacggcagtt ggccaggtgg ttcttcacgg agtcgtgcag aacggactcg
      961 ttgggctcaa caccgaaaac ggcttcggag agcgccactt cgccgacctg atcgccggcc
     1021 atattaaaca acttagcagt aggcatattc gctctctccc ttccttactt tcttgcagaa
     1081 gccttctgcg ggttacgacc ggaggccttc tgcgggttgg tgctgacgcc ggcgtcgccc
     1141 ttcttgacga ccagcttctt aacggtgctc ttgatataga caatgccgcc cttcgggccg
     1201 gggatcgcgc cgcgcacaac gatcatgttg agctcgggat cgaccttgac cacgtcgagg
     1261 ttctggacag tgacctgatc cacgcccatg tggccggcct gcttcttgcc cgggaagata
     1321 cgggaaggat cggtgctcga gcccatggag ccggcgtgac ggtgcacagg gccgccgccg
     1381 tggctggtcg gggtacggcc ctggccgaag cgcttgatga cgccggcgta gccgtggccc
     1441 ttgctgatgc cggtgatgtc gaccttgtcg ccttccttga aggtgtcggc cttgatgaca
     1501 tcgcccacat tgacagaggc gcagtcctcg agacggaact ctttgaggtg cttcttatag
     1561 ccgacgcccg ccttgtccag gtggcccttc tcgggcttgg tcagcttctt ctcggcgacg
     1621 tcctcgtagc cgagctggac ggcttcatag ccttcctttt ccttggtcat cttgccggtg
     1681 accgtgcagg ggcctgcctc gatcacggtg acggggacga ccttgcccgc ttcatcgaag
     1741 atctgcgtca tgccgacctt tttaccgatg atggctgttt tcatacatgt tcctccttca
     1801 ggttattggt cagctcgctt agctcatggc cagaggctgc tgacttgacc cgtccgcaca
     1861 cgcaagctgc ggactgcggg tttgtaaata tgcgctcgcg cgcagggtta acggggtgtg
     1921 cttacagctt gatgtcgatc tcgacaccgg ccggcagctc cagat
//
LOCUS       DAWBMW010000247         1962 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_10892_length_1962_cov_2.410068, whole genome shotgun sequence.
ACCESSION   DAWBMW010000247 DAWBMW010000000
VERSION     DAWBMW010000247.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1962)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10892_length_1962_cov_2.410068"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          414 a          649 c          584 g          315 t
ORIGIN      
        1 tatggccgca gtcggcggcc aagtcgccac cctgaccacg cccaacggca cttacgaggg
       61 cgtgccgatc gccaagttcg gcgagcatca ggcccacaac gcgcttgccg cgctcgccgc
      121 ctccgaagtg gtgattccgg tcaacggtcc gctggacggc gacttggtgg ccgaggcgtt
      181 gagctcggtc aagattcccg gacgcatcga acagatccgc acctcaccga ccatcattct
      241 ggatggcgga cacaacgtga atgccgccga agccctgcgc aaggccatcg aggaaagcta
      301 cgacttcaag cagctggtcg gtgtggtcgc catgatgcgc gacaagcagg tcgaggaata
      361 tctgggtgtt ctggagccga tcctgagctc cgtggtggtc accgagaatt cgtggcgcga
      421 gcgcgtgatg ccggccgacg agcttgagaa gattgccgtg gacgtgttcg gtcgtgaccg
      481 tgtgatcaag gaagcgaacc tgcctgacgc gattcagacc gccgtcaata tggtcgatgc
      541 cgaggacgag ctgggcgtgg gctacggcca cggcgtgctt atctgcggca gcttcgtcac
      601 cgccggcgac gcacgcctga tgcttgagga acacgccagc cccacgatga ggcaggctat
      661 ggccgttcat cagccggcag ttgacccgga cgattcggat cagccggcag acaaggccga
      721 ggatgaggcc gccgacaacc tcgaggattc ggtaagcccg gacgacttcg acgtgttcga
      781 cgtcctcggc ctcggcaagg aacaggccag tgacgccggt aacgccggca ccggcacggc
      841 cagtgccgac accgacaccg acgattccgc cgacgcccgg taacccgcat ccaaggcata
      901 tcggataccc ggacacccgg catacggaaa acagtctcac gccatgtatc tcaaggaact
      961 caccctccgc ggattcaaat ccttcgcctc cgccaccacg ctgcgcttcg agcccggcat
     1021 cacggcggtc gtcggcccca acggttcggg caagtcgaac atcgtcgacg ccctgatctg
     1081 ggtaatgggt gagcagggcg ccaaaaacct gcgcggcact tcgatggagg acgtgatttt
     1141 cgcaggcacg tcctcccgcc cgccattagg ccgtgcacag gtgagcctga ccatcgataa
     1201 ttccgatcac acgctcgata tcgattacac ggaagtcacc atctcccgca ccatcttccg
     1261 caacggaggc tccgaatacg cgatcaacgg ctcgcaatgc cgcctgctcg acattcagga
     1321 actcctctcc gataccggtc ttggccagca gatgcatgtg atcgtaggcc agggacgatt
     1381 ggatgcgatt ctgaaggccg acccgtccgg ccaccgtgcg ttcatcgaag aggccgccgg
     1441 catcctgaag caccgcaagc gcaaggaacg cgcgttgcgc aagctggcca ataccgaaac
     1501 gaacctgagc cgactcgacg atttgctggg ggagattcac cgccaactcg gccccctcgg
     1561 acgccaggca cgtatctccc ggcgcgccga cgcgattcag atatccgtac gcgacgccca
     1621 agcccgcctg tacgccgaag acgctcaacg ctcgatgagc cgtcgcgaca cggtacgtca
     1681 agaacttggc gctgtgcgca accagctggc cgtcgcccaa cgtgaactcg cccaagtcaa
     1741 agtacgcatc gaacaggtcg aggcgttgag ctccgaatcc agtccggcca tcgccaaggc
     1801 caaccaatac tggcatgaat tctcgcagac ccgcgaacga ctgaacgcgc tcgcccagtt
     1861 ggccgaagag cgcagccggt cactcgccgg ccagatcgtc accaacttcg gtgaggatcc
     1921 cggcatgctt gtcaaacgag ccgaggaact ggagtctcag gc
//
LOCUS       DAWBMW010000248         1892 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_11354_length_1892_cov_2.115406, whole genome shotgun sequence.
ACCESSION   DAWBMW010000248 DAWBMW010000000
VERSION     DAWBMW010000248.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11354_length_1892_cov_2.115406"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          306 a          606 c          635 g          345 t
ORIGIN      
        1 ctgggatcat cacgatctgg ctcttgttga tctcgtcgac caacgcctgc gtggattcgg
       61 cgacggcggc cggcgtcagg ttgttgacga tcagtgtggt cacatcggcg ccggcacgct
      121 cgaaggcggc ggcggaatcg tactcgcagt tgttgcccgg gaacaccggg atcaccacat
      181 gcggcttggc cacggaagag ccgacgtaca cggtcttctt cggcgcgtgg aagtcgatgg
      241 tctcgacggt cgcgcccttg tcctcgccct tggaacggta cgggaacacg gattcgatgc
      301 cggactccca agcttcctgc acagcgtcca ggtccagcgt ctcgccggcg gccttgaaca
      361 cgtacccgga ggtggtggtg ccgatttcgc cgatctcgac caaattggag acggccggaa
      421 gcttggcatc gtccgccagc tcgacgatga acgaaccgta tgccggggtg aacaggtcgt
      481 ccacggcgat gctgttgttc agggtcacgc cgatgcggtt gccgagcgtc atcttgaaca
      541 gggcttcggc ggtggcgcca tagcccggcg tagagacggc cagggcatcg tggaagtcgg
      601 tgagctcctc gatcacggag aacacctcaa gcagcgcatc cttgtccggg gtcagaccgt
      661 cggccaggta gcgcggcgcg atgcgtacga tacggtgatc ggcgtccttg aattcagggg
      721 aggtggcgcg cttcatgttg cccacggcca cggcgaagga gatcagcgtc ggcggcacgt
      781 cgagatcctc gaacgaaccg gacatggagt ccttgccgcc gatggcgccg gcgcccaggt
      841 ccacctgggc catcagcgcg ccgagtacgg cggccgccgg cttgccccag cgttccggct
      901 cgtcgcgcag cttctcgaag tactcctgga agctcaggta ggccttctca tgctcgaagc
      961 cggccgcgac cagcttggcc agcgactcga ccacggacag gtaggcgccg gtgaactggt
     1021 tcttggacat gatgtacggg ttgaagcccc acgccatcgc cgaagcggtg gtggtctcgc
     1081 cgaacaccgg cagcttggcg accatggcca tgttcggggt gagctgacgc ttgccgccga
     1141 acggcatgag cacggtgccc gcgccgatgg tggagtcgaa gcgctccgaa aggcccttgt
     1201 tggaggccac gttgatgtcg gtgaccagct tgttcatgcg ttcggccagc gtgccctcgc
     1261 cccacggggt ctcgtaggac tgctgttcct cgacatgcac ggtctggtgc ttggaagcgc
     1321 cgttggaggc caggaactca cgggacagat tcacgatctt gtcgccgttc caggtcatca
     1381 ccatgcgcgg gtcctcggtc acggtggcaa tcaccgtggc ttcgaggttc tcttcgcggg
     1441 cgtaggccag gaactcgtcc acgtcctcgg cggccacgtc cacggccatg cgctcctggg
     1501 attcggagat ggccagctcg gtgccgtcca ggccctcgta tttcttgggc acggtgttga
     1561 ggtccacgaa caggccgtcg gccaactcgc cgaccgccac ggacacgcca cccgcgccga
     1621 agtcgttgca gcgcttgatc agacggcagg catcgccgcg gcggaacaga cgctgcagct
     1681 tgcgctccac cggagcgtta cccttctgca cttcggcgcc gtcgagttcg agcgattcca
     1741 cgttatgcgc cttggacgaa ccggtggcac cgccgatgcc gtcacggccg gtgcggccgc
     1801 ccagcaggat gattttgtcg ccaggggccg gggtttcgcg gcgcacatgg tcggccgggg
     1861 tggcggccac gaccgcaccc acctccatgc gc
//
LOCUS       DAWBMW010000249         1891 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_11366_length_1891_cov_2.070806, whole genome shotgun sequence.
ACCESSION   DAWBMW010000249 DAWBMW010000000
VERSION     DAWBMW010000249.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11366_length_1891_cov_2.070806"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          383 a          547 c          558 g          403 t
ORIGIN      
        1 acccgttcct ggccttctcc gctcagctga tggcaggctt ggacggcatc ctgaaccaca
       61 tcgagcctcc ggagccggtc gacaaggacc tctacgagct gccgccggaa gagcacgccg
      121 gcatcaagca ggtgccgagc tccctggctg aggctatgga cgccctggaa gaggaccacg
      181 acttcctcac cgccggcgac gtgttcaccg acgacctgat cgagacctgg attggcctca
      241 agcgcgacga gatcgaccag gctcgcctgt ctccgacccc gctcgaggac gagctctact
      301 tccacatctg agtttgagag cttagcaacg ttggagttcc gccattgctc ttctcttgtg
      361 ggagtctaat gtgagtcatt cagcgtaact agaaagaacc tcgtgaaatt cgttttcacg
      421 aggttctttt tttgtgtgtt gtgttgtgtg ttatgcgatg tgtgttgctc ctggaatatt
      481 ccagctgcgg gccgctactt agtctcccaa cgctccttga gtacataggc ggcggctttg
      541 ggctgacggt cgcgagtgaa gacgcttctt attgccgtcg acgcgcatga tgccttcacc
      601 ggtttggaag tcgcagaggt tccatacctg ctcgcccact actgcctcga acatgtcgaa
      661 cacttcgtgc tgcatggcga ggtactcaca ttggtactct tcactccatt gcacgctcgg
      721 cagcttgtgc acaccggcat cggtgtccgc accgtattcg gtgaacagga acggcttgtt
      781 ggattcggtt ttcatccatt ggttcatctc gtcgatgaag gtcttcttgg ctccgatgag
      841 ctgataccca tcttgcacat accagccgta ataccggttc aggcagatga catccgcgaa
      901 ctgatggcac ttgtctttgc ctccggcggc catcagcaga ttggtgtagg tcagcgggcg
      961 atgctgcgga tcgagcgtgc gcgcgtaatc gaatatctcc ttgaaatacg gcacggccgc
     1021 ctcggattgg agtcgttggc ggcgtcgatg aaattccaag cgccggaggc cccggcattc
     1081 ttttcgtcga aggggacaat tcagaacaca actgccgaca caatcgtcag ggaggccagc
     1141 gagaacagcg tgcttacgag cacgaagccg accgcatact cgggcatgtt gttcttgcga
     1201 ccggtgaaca tggcgatggt ggtcatggtc ggcagtccgg cgatgaccgt gatcatcagc
     1261 accatgtcat gggtgatcgg cagttgcggc aatgcgccgg tcagcgcggt cagtagcgcg
     1321 tagaaggcga gcgggaatgc gatcatcttg gcgactaggc cagcatagag ttcgtagcgt
     1381 ttgaggacgc cccaccactt ggtcagcgcg aacaggccgc ccaagtagat tagcgacatc
     1441 ggcgtggcca tattgccgat ggtgtgcagc ggcttcagga tgatgtccgg caccttgacg
     1501 ccgagcagaa tcagaatcag ggcgagcatc acgccgacga aggcgggatt gacgaatcgt
     1561 ttaagcaggc cgaggacgcg ggtgcctggc gaggggcgga cgatggccgg agaaccaccg
     1621 tttgcggcat tgctcgccga accagcattg cccaccgaac cggcgccgtt caccgaaccg
     1681 ttcacgaccg cacctcgcgc gccaatcgcg ttgccgccag tcgtttcgtc gaccggctcg
     1741 cacagccata cgccgtacgt ccagagcagc gtctggtcga tgatcgacat cagcgccacg
     1801 taaatcgcgc cgttctgcgg ccaaagtgcc ataatcaacg gaatgccgat gaatccggcg
     1861 ttgccgaaaa taagcgacgc ctgaaataca t
//
LOCUS       DAWBMW010000250         1865 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_11548_length_1865_cov_2.263536, whole genome shotgun sequence.
ACCESSION   DAWBMW010000250 DAWBMW010000000
VERSION     DAWBMW010000250.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11548_length_1865_cov_2.263536"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          406 a          561 c          482 g          416 t
ORIGIN      
        1 aactcatcga tgcgctggat ggcgtggact tggtgggcgt acgctccaag accaacgtta
       61 cagcccgcgt gctggatgcg cgtcctacgc taagcgccat cggctgcttc tgcatcggca
      121 ccaatcaggt cgatctggca catgccggca agcgtggcat cgccgtgttc aacgctccct
      181 actccaacac ccgttccgtg gtggaattgg tgatctgcga catcatctgc ttgatgcgcc
      241 gcattcccgc acacacgcat cacatcaagc atggcctgtg ggacaagtcc gcttccggct
      301 cgcatgaagt gcgaggcaag accctcggca tcatcggcta cggcaacatc ggctcccagc
      361 tgtcggtact ggccgaggcc ctcggcatgc gcgtggtgtt ctacgacatt gaggagaaac
      421 tggcactggg caacgcctac cgttgcgaca ccctcaacga actgctggaa cagtccgatg
      481 ccgtgaccct gcacgtggat ggccgcaagt cgaacaccgg attcttcggc gaagaccaat
      541 tcgcccacat gaagcaggac gcgattttca ttaacctttc gcgcggtttc gtcgccgatc
      601 tgggagctct gaagcagcac ctcgattccg gccatctgtc tggcgcggcg gtcgacgtgt
      661 tccccgttga accgaagaag agcggagatc cgttcgagac ctcactggcc aatgaagaca
      721 atatgattct gactcctcat attggcggct ccaccttgga agctcaggaa tccatcggcc
      781 atttcgtctc tcagcgtctt gaggattact ggttcagggg ttctacctcc ttgtccgtga
      841 acctaccgca gatcaacctc ggtgagtgca agggtgtgtg ccgtatcgcc catctgcacg
      901 acaatcttcc tggtgtgctg gctcatgtca atcatgtgct cggcgaggag aacatcaacg
      961 tttccttcca gtcgctggca accgaaggcg aacttggcta tgtggtcacc gacgtggctc
     1021 agaagcccag cgccgccacg cttgaagcac tgcgcaacat cgaaggcacc attcgcatgc
     1081 gtgtcatcag ctgacgcagc tcatcctcga ttgatatgaa agagacccgt catcatgacg
     1141 ggtctttttc ataagtgctg ggcttcatga ttggctcgca ccatggtttg cgaatcactc
     1201 gcatatcacg gtgcatggca ttcgcatatt attcacgcaa accatcaatg gcgctttgga
     1261 aatcctccag acctgcaaag ttctggtaca cactcgcgaa tcgaaggtac gccaccacat
     1321 ccaagtcacg caacggtttc aggatggctt tgcccacttc atctgaggtg acttcggcaa
     1381 ggcctcggga gcgaagatcc tcttcgactt tctgtcccag cagcttcaga tcctcttctc
     1441 tgaccggccg cccctgacaa gctttgcgta cgccggaaat cactttgtct ctactgaatg
     1501 gctccacgcc acccgaacgt ttagtgacca gcagcatact ggtttccacc gtggtaaaac
     1561 gcttgctgca tttcgggcat acgcgacgcc gacggatgga atgaccgtca tcgctaatgc
     1621 gcgtatcaat gactttggtg tcggggttct ggcaaaaagg acaatgcatg ctccccagaa
     1681 taacgtgtac gggcggaaaa accatgccgc tcgggtttca tttactcaac cggaacaata
     1741 atgcgctgcc cggcctgcaa tgatccggac tcaagattgt tcaacgcaat cagctcatcc
     1801 accgtttccg atacatcatg accagcaggc gtgatagccg aagcatatga ccagagcgta
     1861 tcccc
//
LOCUS       DAWBMW010000251         1850 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_11671_length_1850_cov_2.326462, whole genome shotgun sequence.
ACCESSION   DAWBMW010000251 DAWBMW010000000
VERSION     DAWBMW010000251.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11671_length_1850_cov_2.326462"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          313 a          527 c          580 g          430 t
ORIGIN      
        1 ctcgtagggg gtgtagttct tctggatgaa gtcgcggacg tcaatatcct tctgccagtt
       61 gccctcggtg aagccggccc acgccttggc gtcgagctcc tcctgggaga cagctgcatt
      121 ttccactgcg gtcatgtcac tccttaaatg gggttgtagg tttcgcatct tcacggaacc
      181 cttggttcaa cagtgtagtt ggcacttgct tgagaaaagc tgaaattggg agtgagctat
      241 ctcacattgt ttctgtgaac gctaccattc caatggcttc tagatattga tgggactatt
      301 gcacgtctcc agatatggga aaccgctcat actgtgatgt tcgtatgagc ggttcggtct
      361 tgagattgac agcagaaccg tatccgtcag ttttcgaccg tcaatcctcg gggcgagcct
      421 gtgcgggttt cacgccgtag ttcttccagc gccggttctc ctcctcccat ctggcattgc
      481 ggtccaccac gatgtcccac gcatgttcgg cgtcttcctt ggtcttgtac ggacccatgc
      541 gctgttcgat cggtgagacc atgcccaact cggcctgctt cttgacggtg ttgaaatacc
      601 actgcttctt gttatcgtct gtcattggta cctcctcaac ggtattactt gctcccgctg
      661 gcgggagcaa tatatgttta ttccctgaat gcgttggtga cgggcagtcg gcggtcgcgg
      721 ccgaacgcga gggccgtaac cttcggtccc agcgggtatt ggcggcgctt ccattcggcg
      781 cggtccacca atcgcatcac ggtgtctacg gtgacctggt cgaagccgtc ggctaacagg
      841 tcggcgcggc cgtgcgcgtg ctcgatgtat gcggcgagca ccttgtcgag cagcgcgtat
      901 tcgggcaggg agtcggagtc cttctgcccg gggcgcaact cggccgaggg tgccttctcg
      961 atggaggaga ccggaatcat cacgccgtcc ttcaacggga tgccggtgtt gccgtcctcg
     1021 ttgcccacga tcttgagacc gccgatgccg acgcctgcgg ccgcggcctt gttacgccaa
     1081 cggctgatct cccagacacg ggtcttgagc aggtccttga tgggtgcgta gccgcctacc
     1141 gcatcgccgt agatggtcga gtagccgcag gcgagctctg acttgttgcc ggtggccacg
     1201 gcgagcagac cgcgcgaatt ggagctggcc atcacgatca caccgcggat gcgggcctgc
     1261 aggttctcgg cggagacgcc atcaaggtca agttgctgtt ggtaggcgtt gaacaatggc
     1321 tcaatgggct ggacctcgta atgggcgcca atgttggagg ccaaatcggc ggcgtcgtct
     1381 ttggaaccgt cggaggagta catggagggc atggagatgc cccagacgtt ctcgccgccg
     1441 catgcgtcgg ccgccatggc ggctaccagt gcggagtcga tgcctcctga gaggcccagc
     1501 gtcacgccgg tgaagtggtt cttggccatg tagtccttga ggccgagcac gcaggcggtg
     1561 tatacctctt cgtccgggtc gagttccgga acgatctcgg ccttggcctg atgttcggcg
     1621 gcggaatcga agtcccagaa cgtcaggttt tccatgaaca tcgggctgcg ttcgataagc
     1681 gtgccgtcgg cgtcgaccac gaagctgccg ccgtcgaaga cgaggtcgtc ttgaccgccg
     1741 acctgattca ggtagatgac cggggcgtta acctcggcgg cacgacgctg ggcgagctcg
     1801 aaacgggtgt tggtcttgcc ttcctcatac ggcgagccgt tgatggtgag
//
LOCUS       DAWBMW010000252         1823 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_11868_length_1823_cov_1.813348, whole genome shotgun sequence.
ACCESSION   DAWBMW010000252 DAWBMW010000000
VERSION     DAWBMW010000252.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11868_length_1823_cov_1.813348"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    581..590
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    953..962
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          390 a          581 c          527 g          305 t
ORIGIN      
        1 tgctcaccgt ggtccccggc gtctccggct ccggcaaatc ctcgctggtc aacacgatcc
       61 tctacccggt gctcgccgac aagctcaacg gtgcacgcat cgtacccggc cgccacacgc
      121 gcgtggaagg cgtggaccag gtgcgcaagg tgatccacgt cgaccagaac ccgatcggcc
      181 gcacgccgcg atccaacccc gccacctaca ccggtgtgtg ggacaagatc cgcaccctgt
      241 tcgccaagac tcccgaagcc caggtgcgcg gctacggccc cggccgcttc agtttcaacg
      301 tcaaaggcgg ccgatgtgag gcctgccacg gtgacggcac gctgaagatc gaaatgaact
      361 tcctgcccga cgtctacgtg gactgcgagg tctgtcacgg caaacgatac aaccgcgaga
      421 ccctcgaagt cacgtacaac ggcaagaccg tggccgacat cctcaacatg cccatcgagg
      481 aggccgccga cttcttcaag gcgtacaccg gcatctcccg atacctgaag accctcgtgg
      541 acgtcggcct cggctacatc cgacttggcc agcccgcacc nnnnnnnnnn cccgcaccca
      601 ccctgtccgg cggcgaatcg cagcgcgtca agcttgccac cgagctgcag cgccgatccg
      661 acggcaaaac cgtgtacatc ctcgacgagc cgaccaccgg cctgcacttc gaagacgtga
      721 acaagctgct caaggtgctg cagtccctgg tggacaaggg caatacggtc atcgtcatcg
      781 aacataatct ggatgtgatc aaggaggccg actggctgat cgatctcggt ccggagggcg
      841 gcgacggagg cggcaccatc gtcacgcagg gcacgcccga acaggttgcc gaatgcgaag
      901 cctcatggac cggcaagttc ctgaaaccca tgctttagcc ttccattgca gcnnnnnnnn
      961 nngctcaggc caagtcttta cggtccagca acacacaccc caacccaccc aatccctgat
     1021 gattggctta atagcgagga ggattatgac gaacgatatt gagcggcata gtccggatga
     1081 gtggcggaag actgcggcgg cgttaatgtc ggaaagcata gctgtgggtg ccggggaaac
     1141 gacggcgaag gtgaatgcga atggggcacc tttgctgggg gattctcgcg acctgttccg
     1201 acccaagacc tcggatattc ccgccaaacc cggtgtgtac aaatggcgcg acggggaagg
     1261 gcgcgtcatt tatgtgggca aggcaaagaa cctgcgcaac aggctcacca attatttcca
     1321 gccgctgtat ctgctgcatc cgcgcacgca gaccatggtg ctgaccgcac gcagtctgga
     1381 gtggacggtc gtcgccaccg aactggaatc actgacgttg gaatacacgt ggatcaagga
     1441 attcgaccca cggttcaacg tgcagttccg cgacgacaag acctatccgt atctcgcggt
     1501 cagcacgggg gagcgaattc cgcgcgtgtg ggtcacgcgg tcgcgcaaac ggcgggatac
     1561 ccggtatttc ggcccctatg ccaaagtgtg ggaattgcgg cacagtcttg accggttgct
     1621 gcgcacgttc ccggtacgca cctgcaccac caacgtgttc cataaggcgc aactgaccgg
     1681 acggccatgc ctgttcgctt ccatcggcaa atgctccgca ccctgcgtga accgcattga
     1741 agcggacgaa caccggcggc tgtgcgaaca gctggtcggc gtgatgaccg gacgactcgg
     1801 acggtcatac atcgcccagt tga
//
LOCUS       DAWBMW010000253         1811 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_11959_length_1811_cov_2.477221, whole genome shotgun sequence.
ACCESSION   DAWBMW010000253 DAWBMW010000000
VERSION     DAWBMW010000253.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11959_length_1811_cov_2.477221"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          362 a          596 c          518 g          335 t
ORIGIN      
        1 cccgacgctg aaggccatca gcccgattcg cgacctgtcc ctgacccgcg acgtcgagat
       61 tgcgttcgcc aaggagcaca agctgccgat cacccagacc gagaagtccc cgtactccat
      121 cgaccagaac gtgtggggcc gcgccattga gaccggcttc cttgaggatc cgtggaacgg
      181 cccgaccaag gactgctact cctacaccga cgacccggcc ttcccgccgg tcgaagatga
      241 agtcgtcatc gagttcaagg aaggcgtgcc ggtcaagatc gacggacgtg acgtcactcc
      301 gctgcaggcc atcgaagaga tgaaccgccg cgccggagcc cagggcatcg gccgcatcga
      361 cctcatcgag gaccgcctgg tcggcatcaa gtcccgcgag ctgtacgagg ccccgggtgc
      421 cgtggctctg atcaccgctc accaggaact cgaaaactgc tgcctcgaac gcgaacagca
      481 ccgcatcaag cgcgacatcg acaagcgctg gggcgagttg gtctacgatg cgcagtggtt
      541 ctccccggcg actcagtcgc tcaacgcgtt catcgaggac acccagaagt acgtgtccgg
      601 tgagattcgc atggtcctgc acggcggtcg cgccgtcgtg accggccgtc gttccgactc
      661 ctcgctgtac gactacaagc tcgccaccta tgattccggc gataccttcg accagaagtc
      721 ctccaacggc ttcatcgaca tctacggcct gccgtcccgc gtggccgcag cgcgcgacgt
      781 caagttcggc aacggcatcg aggtcccgga gaacaccgtc gagtaatcat ttactttggc
      841 tcccctcgtt gaggggccgc gccgtaaagc aaacagccca gtgggctgtt tgcaggtgag
      901 gggcgaacga cagtgagcca acaatcagca gccgtcaggc tgtccgtaat tacaggacgc
      961 tgtcgccgca gatgactgag gggagtacga gcaaagatta tgctcgtgct cccctcgttt
     1021 ttttgccgat gacggaagaa gctcagtgct ctccgtaatg agttcggcag acggctattt
     1081 ccaggttttg aacatcgtta cccgtaatgc ggtatacgat gcggttcttt tcatcaatac
     1141 gacgtgacca gtatccctga cattgccgcc atggaatacg aacaacttga atccaccggt
     1201 atgtagccca acgctgatag tctgacaagc agtctgattt gtttgcaatc catggtttca
     1261 aggagccttc atgactgaga acaacgaaca tctggccctg tggggtggtc gctttacgtc
     1321 tggtccgtcg ccggaattgg cccgactgtc caagtccacg cagttcgact ggcgcttggc
     1381 cgacgacgac atcgccggtt cccgcgccca cgcccgtgct ctcggccgtg cgggacttct
     1441 gaccgccgac gagctgcagc gcatggagga tgcgctcgac acgctccagc gccacgttga
     1501 cgatggctcc ttcgccccca tcgaagacga tgaggacgag gccaccgccc tcgaacgcgg
     1561 tctgattgac attgccggcg atgaactcgg cggcaagctc agggccggcc gttcccgcaa
     1621 cgaccagatc gcctgcctga ttcgcatgtg gttgcgccgt cactcccgcg taatcgccgg
     1681 actcctgctt gacctcgtca acgccctgat cgagcagagc gaaaaggccg gccgcaccgt
     1741 gatgccgggc cgcacccaca tgcagcacgc ccagccggtg ctcctcgcgc accagctcat
     1801 ggcccacgct t
//
LOCUS       DAWBMW010000254         1777 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_12237_length_1777_cov_2.184088, whole genome shotgun sequence.
ACCESSION   DAWBMW010000254 DAWBMW010000000
VERSION     DAWBMW010000254.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12237_length_1777_cov_2.184088"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    805..814
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          302 a          521 c          575 g          369 t
ORIGIN      
        1 gattgcaggt cggagatggt ctcgcgcgca agctgctgga atcgggccac gctcatcgtg
       61 tcttcggggt cgatgatgtc gatgagatgg tgcggcacgg ccgcctgttc ctcggcggtg
      121 ggtttggcgg tgccgatatc catgccacgg tacatctggt aggcgtccgc gttcacgatt
      181 tcggcccgct cccccgcttc ggccagacgc cgggcgatgg cgatgcccaa ccccgtcttg
      241 ccggacgcgg tgggaccgac gatggagacg actcgttgcg tcatatacgc cccctcagtg
      301 ccgcacggaa taggtctggc cggcggccac gtcggggtcg gcaagcagat tatggcggcc
      361 ggcgtgtgtg acggtggccg tgacgaaatc gccgatttca ggtaccggtt cgccttcagg
      421 cacgccgatg tgcaccagca cgccggtttt ctcgcggccg gtgacacggt gggtgtcggt
      481 gtccttcttg ccgagtttgc cggtgatcat cacttcgacg tcgcggccct cgaatgtggc
      541 gaggttctct tcggtgatct gctcctgcaa cgccaccaga cggtcgaacc ggtcctgcac
      601 cacgtcgcgc ggaatctgtt ccattgcggc ggccggcgtg ccggggcgtg gcgagtagat
      661 gaacgtgaac gccgaggaga agcgcgcctg gcgcaccacg tccatggtct gctggaagtc
      721 ctcttcggtt tcgccgggga agccgacgat gatgtcggtg gagatctgcg cgtcgggcat
      781 ggcggcgcgg atgcggccca gtatnnnnnn nnnnggactg cagcgggaag tgcagctggt
      841 gcatgatgtt cggcgtttcg gccatcgcgg cgatgacgtc gtcggtgaag gcggccgggt
      901 gcggcgaggt gaagcgcacg cgttcgagac cgtcgatggt gccacaggcg cgcaacagct
      961 tgctgaaagc gtatcgatcg ccaataccat aaccgaacga gttcacgttc tggccgagaa
     1021 gcgtaacctc cttggcacca tcggccacac attggcgaat ctcatcaaga atgtcaccgg
     1081 ggcggcggtc cttttccttg cctcgcgtgg tgggcacaat acagaacgtg caggtgttat
     1141 tgcagcccac ggaaatcgcc acccacgagc tgatgcggga ggcgcgggcg gcgggaagct
     1201 ggctcgggaa ctgccgcaat tgttcagtga cctggacctg cgccttgccg gtcgcacgat
     1261 tctgatccaa cagcttgggc agatcttcga tgttcttggt accgaacacc gcggaaaccc
     1321 atggcgcagt atccgcgatc ttcttgcggt caagctgggc catgcagccg ccaacggcga
     1381 tctgcaggtt cggccgaacc agtttgacgc gattgaatcg gccgatggtg ccatacatgc
     1441 gttcggctgc gttctcgcgt accgcacagg tattgagcac cagcaaatcg aggtcattgt
     1501 cattgatctg gtcctcggtt gcaggcacgt agccattggc ctccagcatg ccggcgatgc
     1561 gctcggaatc gtgcacgttc atctggcagc cgagcgtatg gatatggaac acgcccttgc
     1621 cgcgtttccc cagcaaacca tcggttccat cggctgcaat cgaggcgcgc tccgcctcgg
     1681 tcatcatgtc ttcattcata acgaccgatt ctatcgacgg ggttcgtcat gctccggtca
     1741 cgtatgccat tggttgtcac gatcttaaat atgggtt
//
LOCUS       DAWBMW010000255         1768 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_12317_length_1768_cov_1.876824, whole genome shotgun sequence.
ACCESSION   DAWBMW010000255 DAWBMW010000000
VERSION     DAWBMW010000255.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12317_length_1768_cov_1.876824"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          387 a          469 c          534 g          378 t
ORIGIN      
        1 ggctaccgct tccgcggcaa cgtggtgctg cacgttgacg acgacatcta caacgagcag
       61 gtcaaggtcg ccgaggaaaa gggcaccaag aagccggccg tgattccggt tctcgaagtc
      121 accgagatcc aggaccttgc ctccggtccg accgccggca agaccatcgc caaggactga
      181 ttagctgacg tattggtggc ttcctcgtca gagggagcca ctttttcgct ttttagtgcg
      241 cagtactagg tgacggcctt gggcgtcggt ggtggcgaag aggtaggtgt tgccaccgtc
      301 cttgagtttg agttttttgc gcagctgggc cacggttagg gggaaattgc gtacggcgat
      361 gttggcgtgc gtgaggccgg ccaatagctg tttcagttct ttcttgttca tggtggcgat
      421 ggattcgatg gcgaatgacc tgcctggaaa atcggcaata ggttcggccg ccacgaacag
      481 gtggctattc gggctgatgt gatgcacggc gaagcgctgt tcgagaacat caaagcatcc
      541 ggccttcatg attgaggcgt tgggctcgta aaggtacttc caatgcgagg aatcgaagtc
      601 gttcgcgctt tcctcagacg gggagtcttc ggtattgcaa gtattgcgtg cagccaaggc
      661 agaatcgctg atagtggtgt gattgtcgct gttatcgccg gccttgtacg acagcacctg
      721 ctcgtcatta gcacatacca cgagcggtga gacgcaacgg ccgcgcccca acaccagcaa
      781 caattccttg cactcattcc cggtggagac gatatgcacc tcatgtaccg cgccggcgaa
      841 atcggcgacg gtcttatgcc agtcgagcat gggggagagc ttgaccatga ccgtcggtgc
      901 cttggccagc agcatatcct tcagggtcag cacatccggc atgcaatcgg caatcgcgta
      961 ggtacgcgag ccatgatcgt cacggcgggc cggatcgagg aagatcatgg agactcggcc
     1021 gagcgtgtca agcactgctg ttgaatcatc attgatgact tcggcgtggt caaggccgag
     1081 tatcgggaaa ttatggcgag cgatatcaca taaaatggat tgttgttcca catatatggc
     1141 acgattgaac actcgggaca tgtaagaaaa gtcgacgccg aacccgccgg tcagatcgac
     1201 tagggatgtc ggggagtcgt cgtcgactgc ttcttcggcc aacaatcgct gtaccagccg
     1261 agccttgtac tgtgcggtga attggctgga acattgttcc attgggactt gcggcgggaa
     1321 aatcagccca tcatgggacg cccagtcagg tagcttgagt gaagcgcgct gccagccggc
     1381 aatttgctcc agcgcgaacg gcacatccac gtcgccatca tgcttggcgt gcaaggccac
     1441 atcacgcaca tcttcacggc ggtgtttggc gatgaacgcc caagtcgact cagaaatctc
     1501 catgccgcca ttctaggggt tgtgctgaga tgagtatggc aaaccgcaag agaacgaggt
     1561 ggcgaaaacc gatgatcgat gaagtgatga tgctgcgtca tggccgtacg caatacaatc
     1621 tcgaacatcg cttgcaaggc cagatcgatg tgccgctcga catcgtaggc caatggcagg
     1681 tcgaccagac cggattcgcg ctcgcttcac ggtattactg ggccaaggtg aaccgccttg
     1741 ccgagcatcc cgaagccatc tcccagcc
//
LOCUS       DAWBMW010000256         1749 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_12497_length_1749_cov_2.494097, whole genome shotgun sequence.
ACCESSION   DAWBMW010000256 DAWBMW010000000
VERSION     DAWBMW010000256.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12497_length_1749_cov_2.494097"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          327 a          534 c          506 g          382 t
ORIGIN      
        1 ttcttggcct catcgaacag gtcacgcaca cgggaggcac cgagacccac gaacatttcg
       61 acgaagtcgg agccggccat cgagtagaac ggcacgccgg cctcgcctgc gatggctcgg
      121 gccagcaacg tcttacccgt gccgggaggg ccgtacagca gcacgccgcg cgggatgcgc
      181 gcacccagcg ccttgtactt ggacggatcc ttgaggaagt ccttgatttc ctcgacttcg
      241 gccacggcct cgtcttcacc ggccacatcg gcgaacttgg tggtgggggt ctgcccgtcg
      301 agcagtttgc cgctgttctt cctgccgctc atgccgagca tgccgccggc accacccatc
      361 cggctcatca gccaccagaa cagggcgaag aagatgatga acggcaggat cgaggtgacg
      421 aggtagctca gcatgctggt ctgctcgatg ttcgaggtcc agcccttttc gagatcgccg
      481 ttctccacgg ccttggccac ctgtgcgccc tgggcgaagg tgtagtagaa ctgcacgttc
      541 ttgccgtagt tggtcacctt gccggtgtcg gcattcttct tggtgtagtc gttcttcagc
      601 ttaaggcgga cgacctgctt gttgtccgta atctcggcgt acgtggcctt gccctgattg
      661 atcagcgcga aaccatcttt ggtgtcgatg gttttggtgc caccgccggc gaacatctgg
      721 aacatcagca cgaccatgac cacgagtacg gcaccccata gccaagggga ctgccatatc
      781 ggccggttct cgttgttgtt gcctttgcca ttggtcccgt tattgccacg gttgaacggg
      841 ttggtgaacg ggttgccgtt gttacggttg ttgtccgggt tcggattatt gccgttaccc
      901 ggcccttgcg ggaagctcat gcttgcactc cttggtagac ggacggcttg aggaccgcga
      961 tcgaatcgag gttgcggtag cgttcgtcaa agtcgaggcc gaagccgacg acgaactcgt
     1021 ccggaatctc atagccggcg tacttcacat caacgtccac ctctcggcgg gaaggcttgc
     1081 tcagcaaagc gaagacctcc acggaagcgg caccacgacg tttcagttct gcgaccagcc
     1141 acgcgagcgt gtgtcctgaa tcaacaatat cctccacaat caagatgtgc cgtccgcgaa
     1201 catcggcgga caaatcctgc ctgactgtga cctcaccgct cgacactgtg cccgagccat
     1261 agctcgacag gctcatgaag tcgatctgta caggaatgct catggcctgg gtgaacgcga
     1321 ccagcgtatt cacggcccct ttcagcaccg caacgagcag cggattctta tcgcggtaat
     1381 cctcgctcgc gagagcggcc atctcgtgga tcttggcatc gatctgttct ttgctgacca
     1441 gctcgtggtc aatatcttcc tcaacgtcag cgattcgcat gcgctccatc ttggcacacg
     1501 cgaatgacgt gtttcttgcg attggctgaa taacctctgg gaagtgagac gccgccttgg
     1561 ccatgccaat cgacgaccag ccggtcgatg gcggcaatct gggcggcact ggcgttcaca
     1621 tcggcctggc tcagcgcgtg agcgatgacc cgcaaccgaa tcgcctctgg ctcacgtgca
     1681 atacgtttga cgccgatcat caggccgatg ccatggcccg aatcgttggt atcgtctgca
     1741 gcacaggct
//
LOCUS       DAWBMW010000257         1732 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_12655_length_1732_cov_2.777579, whole genome shotgun sequence.
ACCESSION   DAWBMW010000257 DAWBMW010000000
VERSION     DAWBMW010000257.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12655_length_1732_cov_2.777579"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    618..627
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          299 a          528 c          555 g          340 t
ORIGIN      
        1 tcgcagcaac gcggcattgc cggacttgag gcacaggctg gccacgtcca cagtgacgtt
       61 cggccgggcc tcgtagatca tgccgatgac gcccatcggc acgcgcgtct gggtaaggcg
      121 cagaccgttg ggcaggttgt atccgcgcac gatctcgccg accggatcgg gcagggtggc
      181 cacatggcgc acgccttggg cggcggcggc cacgcgcggc acgtcgaaca gcaggcggtc
      241 gagcttgccc gcatccatgc cgttttcctt ggattcgagc atgtccagcg cgttggcggc
      301 ttcgatgtcc gcagcatgct cgtccaacgc gtccgcaatg gcaagcaaca gttcgttttt
      361 ggcttcggta tttgcctgtg caagacgctc ctgtgcgcgt gcggcctgat cggcctggcg
      421 gcacaccgcg tcgaacacct caggactcaa ttcatcgctc ataaaaaaca agagtaaccc
      481 acaatgggcc gcattgcccg tttcgaccca tattgcaccg aagatagccg gggatataag
      541 cgcggctccc tctgatgagg gagctgcgta catccgcaaa aaatcagtga atctggtcca
      601 ggtcggggta gagcgggnnn nnnnnnntgc cgattctcag gcccgaggcc accgaggccg
      661 ggcgcgggtc gaagggcacg gtgttgcggt tgatggtgat gccgcaggcg gcgagcagat
      721 cctcgccctg ctgcccgtcc atctcgctgt tgcgcaggtc gaccatcacc aggtgcacgt
      781 cagtgccgcc ggtcagcacg ctgatgccgt tggccttgac gtcgtcggcc agtagtcgtt
      841 cggcaaggat cttggcaccg tcgaacgtgc gctggcggat gatctccggg tcggtgcgga
      901 aggtctcggg gttcacgccg tacgcctcgg cgtggtagaa acggccgggg tagccttcgg
      961 cgtatttgtt ggtcagcacg gagccctggc attgcagtac cgctcgcggc acgaagttct
     1021 cagaggcgat catctcaagg ccactgcgct ggcggcccag ttcggagtcc agcagttcgg
     1081 cgatctcagg atcggtttcg cagatcggcg cgttgaaagc atcggttgcc ttctgcgcaa
     1141 ggggtgaagc ggtcatgggt gctctttggt ataagcgaca attgattcgc cgttgtcgcc
     1201 ttaccgtatg gcccgtttgc agccggcctg tttcatgata tggccggtcc ccgttcgatg
     1261 gcaatggtga ttttcgccgg tgcgaccggt cggttatgcg agacggacgg cccttctgcc
     1321 cacggcccac gctagactga tgtggattca tcatcccttt caggcagaag gattcatcgt
     1381 gacaaccacc ttccattcca cccgtagcac caccgattcg ctgaccgcca agcaggcgat
     1441 tcgtaagggc atcgccgatg acggcggcct gttcgtttcg gacgcgttgg gcaagaccaa
     1501 ggtggacgtg gcctccctgc cgggcaagtc gtaccagcaa atcgccgccg aggtgctggg
     1561 cgcgctgctg ccggacttca cggccgagga gctgggccag tgcatcgccg acgcctacgg
     1621 cgaacagtgg tccgacgagc gcatcacccc gctcaagccc ctgggcgacg attacgtgct
     1681 cgaactgttc aacggcccga cttccgcgtt caaggacgtg gcgctgcaga tc
//
LOCUS       DAWBMW010000258         1699 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_12939_length_1699_cov_2.197689, whole genome shotgun sequence.
ACCESSION   DAWBMW010000258 DAWBMW010000000
VERSION     DAWBMW010000258.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12939_length_1699_cov_2.197689"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          331 a          537 c          515 g          316 t
ORIGIN      
        1 cgccttccca cacgtgttcg cgcttggcga cgtccagcag cgtgcgcgtg gcaagcggca
       61 tggtgtcgat ggccacgcgg ttgtgtgtgc cgcgccggcg catggatggc atgccgttgt
      121 cgtcggcaat catttcgatg cgggtacgcc aattgaggcc gccggctgcc ttgtcgccgg
      181 gcatgcgctc gatgggaacg gccacgtcga tatggccgag tcggctcatc tgttcggaga
      241 cggtgatggc cttccacttg agctggccgg gcaaagagac gtgcacgaga tccgcgccgc
      301 ccactccccc gcccatcgcc aagggaccgg caagtggcca tgccggggtg actcggtctt
      361 cggacggctc gagaacctcg acgacttcgc cggtccagaa ccggtcgtcc cggtcgtggg
      421 gctcgtcaag ttcgactcgc acgagttcgt cgggcaaggc gaagcggacg aacaccacac
      481 gaccgtcgat atgggccacg cagcggcctt ggtcggcgta tcgttcgata cgaacttcag
      541 cttgcatcat cgtcctttcc ggttgcgctc ggttcgagtt cggcatcaac agcgacttca
      601 gtctgtatct tcctagccgc gaccttatgc cggtgcatcg gcgtggcaat aatgaggtaa
      661 ctcacccaaa tcgccaatgc ccagaacgga atgcccatga gcaggcgggt cacgccgagt
      721 gcagctacat ggttggtcag atacagcggc acctgcacca ccagccgcag caggaacaga
      781 ccgacccaca ggccggtgac aatcatatag gcgcgtttca gggccttgtc gtccatccag
      841 tcatggaacc atgccttgaa atgctcggtg ggcaggctac ggatgaattc gacgaccaaa
      901 cccagtccgg gcacgcgcgc aatcagcgac acggcaagca acgcggcata gccagcattg
      961 gtaatgaatc cgaagatgta gtaattgcgg gcctcatgag tctgccatgc ccaaatcagg
     1021 cagatgccta ccgccaccag accagagacc gcgcccatta ccgactggcg ctgaatcagt
     1081 cgcacaataa cctgcaatac cgccaacacg gccgaaaccg caatggtgag attcagattg
     1141 ctcgtggcca cgaacagtac gacgaacacc acgccaggca gcatggattc gatgacgcca
     1201 cgcgggccac cgatggcgtc gatgacggag aaatcctcgc cttcattgcc ggcgtcggct
     1261 agagcggaca ggccggtgcg tttgttgtct gccaagtctc ttcctataaa tcagcgaact
     1321 tcggagaaca tcgggccgcg agacagcgtg gtcttcactt cggtctgctg gtcctggctc
     1381 aacgggccct tcggcttgtt gtccggaatg tccttgaagt gttccttgcc gttcgcctcg
     1441 ccggcttcgg ccgcggcctt ggcggcctta cgctcagcgg gggcgaccgg cggatgcatc
     1501 gggatcagat cgcgcggcgc caaaggctcc tcgccacgct cgacgacgat gccggcgaag
     1561 aacttgttca gcgcctcggt ctccgggctg tccggatcga tagcagcctt gccggagaag
     1621 atgccacgca gcatccagcg cgggccgtcc acaccaacga tgcgcgtgtt cacggttttg
     1681 ccgcccttga cattgaccg
//
LOCUS       DAWBMW010000259         1695 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_12975_length_1695_cov_2.877439, whole genome shotgun sequence.
ACCESSION   DAWBMW010000259 DAWBMW010000000
VERSION     DAWBMW010000259.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12975_length_1695_cov_2.877439"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          343 a          505 c          527 g          320 t
ORIGIN      
        1 tgacgtgcat gattgcccgc aggcccgcta tgagtcctat cagggcgcga agatcacgcc
       61 cggcatgatc ttcaccatcg agcccggcct gtacttccgc gaggatgatc tgctgattcc
      121 gccggaatac cgcggcatcg gcatccgcat cgaggacgac gtgctcatga ccgagaacgg
      181 ccccgaatgg atttccgccg gcatcccgaa gcagatcgac gacgtcgaag cctggatggc
      241 cgagcaggct gcccaagcga agtaacctaa gctccctctg atgagggagc ttcttttcta
      301 ctaaggaggc aatgtgtcca ctccggaatt tgtgttggaa ttgcgtaaga agatcggcca
      361 tgatctgttg tggctcaatg gcgtgaccgg ttgtgtactg aacgaacatg gtcaactgct
      421 gttgggccgt cgttcggaca cgggcgaatg ggcgatggtc tacggcatca acgagccggg
      481 cgaacagccg gcggacacgg tggtgcgcga aatcaaggag gagacgggca tcgatgccat
      541 cgtcaccgat ttggtggccg tcacctcgtc aaacaaggta ctgacctacg ccaatggcga
      601 caacaccatg tacatggatc attcgttcct gtgcgccctg aagcccggcg gcaatgccga
      661 gccgttcgta ggcgacgagg aaagcctgag cgtcggctgg ttcgatctgg atgacctgcc
      721 cactcccctg gcggccagca cgagcgagcg gctggagctg ttccatactt acatcgagaa
      781 caaaaaacac ggcgacgccc acgccctgtt ccagttcgat gggcagcagt actagcggat
      841 ataaagttag ctccctctga cgagggagct ccaatgctat gcgattacgc ctcttgggct
      901 aggagctcgt ggaggtgggc gcggtgttgc tgctgttcgc gcgggtcggg cacgggcagg
      961 gaggccagca gcttcttggt gtacgggttc tccgggtgct gcataatctg gtcggcatcg
     1021 ccgtgctcca cgatctggcc cttatgcatc accatgatgc ggtcggcaag catatcgacc
     1081 accgccaaat catgggtgat gaacaggcac gcgaagccga tttccgcctg cagtcgcttg
     1141 aacagctcca acaccttggc ctgcaccgac acgtccaatg cagaagtcgg ctcatcggcg
     1201 atcagcagcg acggcttcaa cgccaaacca cgcgccaaag acgccctctg gcgctggccg
     1261 ccggacagct catgcgggaa acggttcatg taggcgcgcg gcagctggac catctccagc
     1321 agatcgccca catagttctt ggcatcggcc acggaaccgt acttgtggtg cacgagcagc
     1381 ggctcggcca cattctccgc gatggtcatc agcgggttga acgaggagcc cggatcctgg
     1441 aacacgaaac caatgtccgc gcgcttgggc ttgaagtcac gctccttgac gccgttcatc
     1501 tcgatgccga gcacgttcag ggagccgccg gacaccttct gcaggccggc gatggcacgg
     1561 ccggtggtgg acttgccgga accggactcg cccaccaggc ccagcacttc ggagcggtgg
     1621 atggtgaagt tcgcgccgtc cacggccttg aagtcgggct gcatgagatg gcccgggtac
     1681 gtgatggtca ggttc
//
LOCUS       DAWBMW010000260         1686 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_13056_length_1686_cov_2.419988, whole genome shotgun sequence.
ACCESSION   DAWBMW010000260 DAWBMW010000000
VERSION     DAWBMW010000260.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13056_length_1686_cov_2.419988"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          368 a          524 c          471 g          323 t
ORIGIN      
        1 gttcaagcgc gcagtgagtt tctcagccac accacgcgaa ttaacgaaca ccaatgtagt
       61 gcggtgcgcg agaatctcat ccaaaatact gcgttccacc acgggccaaa tggaaccgga
      121 ggtacggtcg cccgccgctc caaccaacgc ggaggaatcg aacgcatcac tcgcatcaga
      181 atcgtcccca gaagtggaag aaacatctct atcatccacc ggcacaatgc ccctgcgttc
      241 agcaagtcgc tgcatggccg gcgtgacacc actgatatgc ggagcggacc gttcagcatc
      301 cacgccaccg acacgctgct tggcattcac ggactgcaaa tcacgcatgt tttccaacgg
      361 ctcaatcatg cgcaaatcca ttgcaggccg cccaccggga ttcacgatgg taaccggttg
      421 cccgccgccc aggaatcgcg ccgccttttc aggcggattc accgtagcgg atagaccaat
      481 gcgctgcata tgacgatcgc cacggccagc atcgacggcg gcatcaccgc cctcatcagc
      541 atctgcatcg attgcatctc gcttgcgcga ttctgtgaca aggttctcca gccgttccag
      601 acttaccgcc aaatgtgcgc cgcgcttggt gcccgccact gcatgaatct catcgacgat
      661 caccgtatcg acagtgccga gaatccgccc tgccttcgaa gtgagcaaca aatacaacga
      721 ttctggcgtg gtaacgagga tatccggtgg atgggaggct atgcgtcgcc gctcctgtgc
      781 ggtggtgtcg cctgaacgag tggcgaccgc gatcttcggc acgggcagcc cttgcgcctc
      841 gcattgtgca gcaatgccct ccaaaggctg ttctaggttc ttggccacat cggccgcgag
      901 cgcctttaac ggcgagatat acagcacacg cacaccggca cgccgcgtgc gtggcacggt
      961 catcaatcgg tcgatggctg agagaaacgc ggccaatgtt ttgccggaac cggtaggggc
     1021 gatgaccaag acattgcgac cggaatggat tgccggccat gcctgagcct gtgcctcggt
     1081 cggctgaccg aaggcatgct cgaaccacat cttggtgggt tcggaaaaca ggctcaggct
     1141 ttcgtacata tgtacgatta taggtcagct gaagtaggcg atgacttctt cggcggtacc
     1201 gtaggcactt tgggcaccaa gcttggtggc cgcataggcg gagacatagg agccgatttg
     1261 cgcggattgc agcagcgtat ggccagcggc caagccagcc agcacagttc ccatgaacga
     1321 gtcgccgcaa ccggtggtgt ccactgcatt gacttgcgcg gcggatacgc gatgccaacg
     1381 tccgttctcg atcaccaccg atcccgacgc accaagcgtc actatcgccc ggtcgaatcc
     1441 gtaatcggtg aatcgttcgg cgacctcata ccagtcgagt gattccacgt catcatccgg
     1501 caggcccagc agttgagcca cttcatgctg gttgaccagc agaatatccg tggcttccac
     1561 cagttcgtga ggcagctcgt ccataaatgg cgagtcgttg agcagcacgg tcacgccagc
     1621 gtcatgcgcg gtctgcgcgg cgacgataac ggtggcaatc gggctctcca gacacagtcc
     1681 caatac
//
LOCUS       DAWBMW010000261         1664 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_13258_length_1664_cov_2.242387, whole genome shotgun sequence.
ACCESSION   DAWBMW010000261 DAWBMW010000000
VERSION     DAWBMW010000261.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13258_length_1664_cov_2.242387"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          364 a          511 c          478 g          311 t
ORIGIN      
        1 gcgttgaagg cgcgcgtgtg gccgtcaaga acgcccagcg caatctgcgc gccatgaacc
       61 tgaacgacgt ggacgcccgc gcgggtgacg tctcgcgcac gctggaccat gtgccggcgc
      121 atctggccaa gccgaatgtc gtagtgcttg atccgccgcg tgccggtgct cgtgccaagg
      181 tctgccgcca gattgccgcg gccggcgcat ccagcgtggt ctacatcgcc tgtgatccca
      241 ccagcctcgc acgcgacacc gcgacactca tcggcgaagg ctacgagctc aaagacatcc
      301 gtgccttcga tatctacccg atgacccacc atgttgagac ggtggccttg tttacccgct
      361 gacaggtgag catatgacac gacgaattct cggattgctg gcctgcatgg cggctgtgtt
      421 gtccctggcc gcctgcacgc cggccggtag ggcggtcggc gatacccaag acagcatgcc
      481 ggaagtcgcc cacgattcca cacatcccac ggatgtcaca gtcggctttg tggggtcgac
      541 tgacaccgcc gcagacgagt tcgtcatcaa tgcgctgtct gatgacaaac tcaatgtcta
      601 ctatgcctcg ctcgaaacat ccgcaagctc ggcaaacgca actgatggtg tatccggtga
      661 aaccgaatct tctgacgccg gcactaccag cgaaaacggt gccgatagta tggatgctca
      721 tactggcgtc gacaagaatg cggtcaccgc acagcagggt gttgacgatt tcgtagctcg
      781 cgcggtcaaa atcgtggtta tcagcggcat cgacgttacc gacgccaacc gggagagctg
      841 gaaccagacg ttgaccaatg cgcgtgaagc cggtattcca gtcgctctca ttgatccaaa
      901 gcatgcgccc gaagatgaac tgctgtacgc cgtcatcctt cacctcaaca ccaacagtgc
      961 cgattctggc gacactgccg ataccaagcc aatgactatt actgacgccg tgctcaccat
     1021 cacgcgcgac gaaccccacg aacgcgagat caaagtcacc gtttcctgag ccgaatcagg
     1081 cccttgaagc tgagaaagca ccctgaatca tccctgagcc ttagcggatt gcggaacaag
     1141 acgcggtagg ctggggaata tgagtgcaac aacagccgtg gaaatgcctg agatcggagc
     1201 gaccggtctt acccccaaag aagtggtgca gcgtatcgaa agtggccaat cgaatgcggt
     1261 gaaaacatcc acctcgcgtt cggttcaaga tatcgtgcga gccaacgtgt tcacgctgtt
     1321 caacggcatc atcttcgccg cgatggtact ggtgctgatc accggttcct ggagggacgc
     1381 ggtgttcggt ttcgtcatca tcatcaacac gggcatcggc atcgtcaccg aactcagggc
     1441 caagcgcacg ctggacaggc tttccatcct tgtggcctcc gaattcctcg tgcatcgtga
     1501 cggcagggac gtggaggtgc cccacaacga gatcgtgctc gacgacctgc tgtggatccg
     1561 tgccggcgaa caggtgccgg ccgacgggca gatcatccag acctggggac tggaactcga
     1621 cgagtccatg ctcaccggcg agagcatgcc ggtggacaaa gcgc
//
LOCUS       DAWBMW010000262         1640 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_13476_length_1640_cov_1.617666, whole genome shotgun sequence.
ACCESSION   DAWBMW010000262 DAWBMW010000000
VERSION     DAWBMW010000262.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13476_length_1640_cov_1.617666"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    458..467
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1080..1089
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1348..1357
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          291 a          481 c          493 g          345 t
ORIGIN      
        1 gaatcggccc gatctcgcta tgcgattcca tgatgacgcg cacgcttttc aggtcggcgg
       61 ctgacgtgct gccagccttc ctgtagatgg cgtccaaatc ctcccggttg agtttcagac
      121 cggggtcatt gttcgcggtg atcggggcga cggtgcattt gcagttgttg tgcatgggca
      181 gaaggtcggc cgtggaaaac acgttcgtgg ccgcgacggc gcacaggccg cacgtgccgg
      241 tcttggaaag ctcggggtgt atgaccctac ggtattttct gacgccggaa ccgtggaatc
      301 gttgcgtggc cgcactgttc atggctatct gaccatcggt gttcgcattg tccgtcaacc
      361 gtttcacggc ggcgtcaagc caatcatcga cggccttctg cacgtaatcg tccagattgt
      421 cccatgccag cgggcgtatc gacgggtccc ttacggcnnn nnnnnnngcg tcggcaggac
      481 gcacgctcac cgcccacggg tcggtgttgt cccttgtgac gatgtattcg ggaatctgac
      541 catccgaagg cacgttcacc atgccgagca tcacgtccgc ataggagacg cccagatgac
      601 gcatggcttt gatgaacgcg atctggttct gtgttatcca cgcggacacg ccctgtgtta
      661 tcgcgtcgtt ccaccagtcg gcggggtcga gcgacttcca catgttccac gcacgctgta
      721 cgtaggcgtc gaccagcgcc tgacgctgcc gttccatgac ggtcagcgcc tgtgtcatgt
      781 cggccatcac gtcacctcat tggtggagtc caacgtctcg tcgcccagaa cgtcgttcag
      841 gtcagggatg gtcgatgtcg aatccaacgt gtcctgcaag atgggagccg actgctgtga
      901 ggtcttgcct tcgaccagag tgttctcctg actcagagcg gtggcgaaag ccgtgtcctg
      961 caagtcctgc atggcttcgg ctatatccat ctcgctcatg ttcaggaacc ggcgcatgat
     1021 ggtcttgacc ggtagcagtc ccttcacata gttggcggct tgcgcctgct ccaaatcggn
     1081 nnnnnnnnnc gaaacgttca tcggcggcgg actgctggcc gcttgcaacc aacgccatgc
     1141 gaagcaacag cacgaacgca tcattggcac gctcgttcat gtcctgcacc ttgagcctca
     1201 acatgcgggt ggtgagcttc gccccctccg cgctgccgga aacgtcaggg ctgagaatcg
     1261 acaacggggg gccggacgcg ccggccaact gtttgatgtc cgtgttcgcg gcggagacga
     1321 tcggcgtgat gtccgtcacg gagctttnnn nnnnnnncca caaggcggcg gggccaagct
     1381 cgaacaagga cgagtagtcg atcttttcgc cggcacgcgc acggttggcc ttcacggccg
     1441 ggtcctgctt cgtgtaatac tcgggaaggt cgccggacac ccaacgctgt ttgaacgcct
     1501 gcatctcctg aatgcagaaa cgttggaaac gctgctggtc gatggcgctc aacgtcggaa
     1561 gatgaggctc gaactggcct cgaccggtcg cggtcttcaa ctggacgatg ggcaggcaac
     1621 cgcagtcacg ggcgaaatca
//
LOCUS       DAWBMW010000263         1639 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_13478_length_1639_cov_2.719066, whole genome shotgun sequence.
ACCESSION   DAWBMW010000263 DAWBMW010000000
VERSION     DAWBMW010000263.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13478_length_1639_cov_2.719066"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          311 a          527 c          488 g          313 t
ORIGIN      
        1 cctccgctct gatggaggct ggcgtggacg tgctcgtggt cgacaccgcc aacggtgagg
       61 cccgtctggc actcgacatg atttcccgcc tgaagcacga ctccgccttc gacggcgtgc
      121 agatcatcgg cggcaacgtc ggcacccgct ccggtgctca ggccatgatt gaggctggcg
      181 cggacgccgt caaggtcggt attggccccg gctctatctg caccacccgt atcgttgccg
      241 gtgtcggtgt cccgcagctc accgccgtgt acgaggctgc ccaggcctgc cgtgccgctg
      301 gcgtgccctg catcgccgac ggtggcatcc actactccgg tgacatcgcc aaggccctcg
      361 tggctggcgc ttcctccgtg atgctcggcg gcacgctcgc tggctgtgag gaagccccgg
      421 gcgagaaggt gctcctgcac ggcaagcagt acaagctgta ccgtggcatg ggctccctcg
      481 gtgccatggc cccgcgcggc aagaagtcct actccaagga ccgctacttc caggccgacg
      541 tgacctccag cgacaaggtc gtgccggaag gcgtggaagg cgaagtgccg taccgcggac
      601 cgctgaacgc cgtgctctac cagatgctcg gtggcctgca ccagtccatg ttctacatcg
      661 gcgcccacaa catcgccgag atgccggagc gtggcaagtt catccgcatc accgacgccg
      721 gtctgcgcga atcgcacccg cacgatatcg tgatgaccac cgaagccccg aactactccg
      781 gcttccacaa caactgatcg gttgatgccc gctagcttgc gctagttagg ggttgattcc
      841 ttgtgaaaag ggtctgctta tgcaggccct tttcgcgtat gtggctcgag atagcttgta
      901 tgcgctgggg tatagtctga actgttcttg taataacggg tgacatgacg aaaagaggct
      961 taccatggtc gacagccatg atgcagaaac ttacagcgca aaggattcgc gcctgatttg
     1021 gattgactgc gaaatgacgg gcttggatat cttcggcggc gacgaactcg tcgaggtgtc
     1081 cgtagtgccc actgactttg acctgaatgt gctggacgag ggcgtggatt acgtcatcaa
     1141 gccgtccgag aaggccgtga accatatgaa cgatttcgtg cgccagatgc acacccgttc
     1201 cggcctgatc aacgagtggg agaatggcct gagcctggcc gaggccgagc agaaggtcac
     1261 cgaatacgtg ctgcgcttca cgccggaagg cgtgcgcccg ctgctggccg gcaacaccat
     1321 cggctccgat aagaagttcc tcgaccatta catgccgaac ctcatgagcc acctgcatta
     1381 ccgcagcgtg gatgtgagca cattcaagga gctcgcccgc cgctggtacc cggccgtgta
     1441 cgagaaccgc ccgccaaaga acggtggcca tcgtgctctc gcagacatca tcgagtcctt
     1501 ggacgagctg cgctactacc gtaaggcgtt tatggctccc gcccccggcc cggacgatgc
     1561 cgcatccaag gccatcgccg ccgacatcgt agccaccagc atcctcaata agtagtccgc
     1621 cgtacttggc tcccctccc
//
LOCUS       DAWBMW010000264         1637 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_13500_length_1637_cov_2.292035, whole genome shotgun sequence.
ACCESSION   DAWBMW010000264 DAWBMW010000000
VERSION     DAWBMW010000264.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13500_length_1637_cov_2.292035"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1397..1406
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          324 a          497 c          482 g          324 t
ORIGIN      
        1 ccatgaactt gtaggcggcg gcgaccttgt ccttgtcgtc ggacttcacg atggccagcg
       61 aggaaccacc gttctcggag ttggtctcgg agccgtctgc ggtcggcatc tgggcgatgc
      121 gccacttgcc gtcaccgttc ggagcgccgg aaagcaggtt gtacggcatc caagcgccgg
      181 tcagcaggga tgcgatggag ccgtcatcca gacccttgtt ccaatcgtcg gtccagccga
      241 cggtcttggt gtcgatgaga tcttcgtcga tcatcttctg ccagaagtcg gtgaaggtct
      301 tgacgttctt gtcaccggtc aggttgatgg tcacggtccc gccgtccttg gaggtctcga
      361 acggggtcgc gccggccagc caggtcatgg agtcgaagaa gccggcgtca ccggaatcgg
      421 aggtgatgta ggagtccacg gcgcgaatct tcttggcggc ctcgtagaag tcgtcccacg
      481 tgcgcacctg ggtcggatcg acgccggcct tgtcgaagac gcccttgttg tagaaccagg
      541 ccatcgggcc ggaatccatc ggcagggcgt agacgccgtc attgatgttc accgaggacc
      601 acgtgcccgg ggtgtagaag tccttgaaat cggaggcacc gagatcggaa aggttctcga
      661 ggtggccgcg aatcacgtat tcgggcagtg cgtagtattc gatctgggcg atgtccggag
      721 caccggagcc tgcctcgatg gcgttggaca gtgcgttgta ctcgtccttg ttggtgccgg
      781 cgttggtcac cttcacctta atgttcgggt tcttggcctc gaatcccttg accgtacgag
      841 gcagtgtgga atcccaggac cacacggtca gctcgacctt cttatcagag gagccggtgc
      901 ttccgccatc cccggcagaa gtggaaccgc aggcagctac agaggagagc atggccacag
      961 caccaatcag ggcaattgtc ttcttgccga acttcatcgt tctttccttc cttgttatgg
     1021 gaaacgacgg gacttggcgt tttgttccaa gccttgtatc gttttaagaa acggtaccac
     1081 atgttttatg tttacgcaaa cacgacacgt cgcaccatgg tgactaaccg caaaccgaag
     1141 gagatgcatc ccgctcatga ccactcacaa cagccagtat tacgccgaaa ccacccatcc
     1201 cgacaagcag gaaagcagcc cggcgccgac cgccgccggc accacggcca gtaacgtctc
     1261 cacaactggc aacgcaacca cgccggacgc cagcatcgcc ctcaacgccg acgccactcc
     1321 ggtagccgac gttcccccgc gtctgttcgg ctcattcgta gaacatctgg gccgctgcgt
     1381 ctacggcggc atctacnnnn nnnnnntggt caaggagctg ggcgtcacct gcgtgcgcta
     1441 ccccggcggc aatttcgtat ccaactacaa ctgggaagac ggcatcggtc cacgcgagaa
     1501 tcgccccgtg cgccgcgacc tggcctggca ttgcaccgaa accaacgaga tgggcatcga
     1561 cgacttctac cgctggagcc agaaagccgg caccgaaatc atgcttgccg tcaacatggg
     1621 cacccggggg ctgaaag
//
LOCUS       DAWBMW010000265         1633 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_13547_length_1633_cov_2.877693, whole genome shotgun sequence.
ACCESSION   DAWBMW010000265 DAWBMW010000000
VERSION     DAWBMW010000265.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13547_length_1633_cov_2.877693"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          317 a          468 c          492 g          356 t
ORIGIN      
        1 cgggccggtc tcagcttcgc cgtcgccgac gatggccggg acaaacaggc tcgggttgtc
       61 catgatggcg ccgtaagcgt gggacagagc gtaacccagc tcaccaccct cgtggatgga
      121 gcccggggtc tccggagcga agtgggacgg aatgccgccc gggtaagaga actgacggaa
      181 gaacttctgc agaccagctt cgtccttggt gatcttcggg aaggtctcgg tgtaggtgcc
      241 gtccaggtag gactgggagg taccggccgg gccaccgtgg cccgggccca tgatgatcac
      301 ggtgttctgg ccgtggtcag caatgaaacg gttgatgtgg ccgatgagga agttcaggcc
      361 aggggtagtg ccccagtggc ccaccagacg gtgcttcaca tcttcgcggg tgaagggctc
      421 cttcatcagc gggttggaac gcagataaat ctggccgatg gaaaggtagt tggcaacgcg
      481 ccagtacttg tcaacgcctt cgagggcttc ctcggaaacc ggagcgttca gcttcttcca
      541 aggggtgcca ataacaggac tcgtcatgtg tactcctgta ctcctgcacg tgatcgtgcg
      601 attgattatt tctgttcggt cacggtccct gacccttgcg gtgccaaggt tttccgttcc
      661 caatcaatcg tcatggtacg aggacaacgc gacttttgaa cgatttcttg cgggtgtgtt
      721 cgaaattgtt gcggatattt cagtatttgt gcggttcgac tgaccgtttg tcaacattag
      781 ttttttcaac atactttttc aaaacggcgg aagcatgcgg aaatccagca tttggctaga
      841 acagcgcacg ttcgatttcc catgttcgaa atcggcgtgt tgtccgtcta caggcatgta
      901 gtttgttcga tttgcgcaca tagtctctgc gtgtcggcct tgtcgccttg accagtgata
      961 atcaggggtg ttcagcccac gtaaaattcc aatgaacgtt aggggaatat caatggcaaa
     1021 cggtcctgtg cttgtcgtcg acttcggcgc ccagtacgcc cagctcatcg cccgccgcgt
     1081 gcgcgaagca ggcgtgtact ccgagctggt ccctcattcc atgccggtcg acgagatcct
     1141 ggccaaggat ccgaaggcga ttattctgtc tggcggcccg gcctccgtgt tcgagcccgg
     1201 cgcgccgacc atagacacca aggtattcga atccggtgtt ccggtgcttg gtatctgcta
     1261 cggcttccag gtcatggcct atgagctcgg cggcaaggtg gataaggccg cgctcggcga
     1321 atacggcaag acctcagcca ccatcgacga cgccgccggc atcctcgccg actcccccgc
     1381 cgagcagacc acgtggatga gccacggtgt ggcggtcgaa caggccccgg ccggcttcga
     1441 agtgctggcc cacaccgaag gcgcgcccgt ggccgccatg gcggacgaat cccgcaagct
     1501 ctatggtgtg cagtggcacc cggaagtcaa gcactccccg cttggccaga agcttatcga
     1561 aaacttcctg caccgttgcg ccgcgctgcc caacgactgg gatgcctcct cgattatcga
     1621 ggatcaagtc aag
//
LOCUS       DAWBMW010000266         1622 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_13663_length_1622_cov_2.134014, whole genome shotgun sequence.
ACCESSION   DAWBMW010000266 DAWBMW010000000
VERSION     DAWBMW010000266.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13663_length_1622_cov_2.134014"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    975..984
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          342 a          511 c          510 g          249 t
ORIGIN      
        1 gtttaccgac cgtgcacggc gcgtgatcgt gttggcgcag gaagaggcga gggcccttca
       61 gcacaactac atcggcaccg aacacctgct gctcggcctg atccgcgaag gtgagggtgt
      121 agccgccaag gcactcgcct cgaagggcgt ggagctggat gccacccgca agcaggttga
      181 agaaatgatc ggcaagggca acgcggctcc gaacgggcac atccccttta ctccgcacgc
      241 caagcaggtg ttggagtttt cgctgcgtga agccctgcag ctgggccaca gctacatcgg
      301 caccgagcac atcctgctcg gcctgattcg cgaaggcgaa ggcgtgggca cccaggtgct
      361 catcaagatg gacgtcgatc tgggcgagtt gcgtagcgcg accatcgata tgattcgcgg
      421 caactccggc accggcaccg gtgacggcaa gggcgacttg gccaacgccg gcggcgtgac
      481 ggacaagcag aacaagtccg gttccgccat cctcgatcag ttcggccgca acctgaccgc
      541 cgaagccgct gaaggcaagc tggacccggt catcggccgt accaacgaga ttgagcgcgt
      601 tatggtcgtg ctttcccgcc gcaccaagaa caacccggtg ctgatcggcg agcccggcgt
      661 gggcaagacc gctgtggtcg aaggcttggc ccagaagatc caagccggcg acgtgccgga
      721 aaccctgaag ggcaagcagg tctactcgct ggacctcggt tccatggtgg ccggctcccg
      781 ctaccgtggt gatttcgagg agcgcctgaa gaaggtgctg aaggaaatca agactcgtgg
      841 cgacatcgtg ctgttcatcg acgagattca caccatcgtg ggcgccggct cggctgacgg
      901 cgcgctgggt gcctccgaca tgttgaagcc gatgctggcc cgtggcgagc tccagaccat
      961 tggtgccacc accannnnnn nnnnggacgc ggctctggaa cgccgtttcc agccgattca
     1021 ggtgcacgag ccgaccatcg ccgagaccat cgagatcctg aagggcctgc gttcccgtta
     1081 cgagaaccac caccacgtga ccatcaccga tggcgcgctg cagtctgccg cagagctgtc
     1141 cagccggtac atccaggacc gtaacctgcc tgacaaggcc atcgacctga tcgatgaggc
     1201 cggtgcacgc ctgcgcatca agcgcctgac cgccccgcct gagctcaagg aactcgacga
     1261 gaagatcgcc aagatcgccg ccgacaagga cgaggccatc aagggccagg acttcgagaa
     1321 ggccgccgaa ctgcgcgaca agcaggagaa gctggaagcc gatcgcaagc agaaggaaga
     1381 ctcctggcgc gaaggcgagt ccgacgtcaa gatggtcgtg gacgaggacg tgatcgccga
     1441 ggtcatcagc tccaccaccg gtatcccggt gttcaagctg actcaggccg agtccaagaa
     1501 actgctcaac atggaagccg aactgcacaa gcgcatcatc ggccaggacg aggcggtttc
     1561 cgccctgtcc cgctctatcc gccgtactcg cgtgggtctg aaggacccga agcgtccgtc
     1621 cg
//
LOCUS       DAWBMW010000267         1579 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_14125_length_1579_cov_1.937008, whole genome shotgun sequence.
ACCESSION   DAWBMW010000267 DAWBMW010000000
VERSION     DAWBMW010000267.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14125_length_1579_cov_1.937008"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          268 a          440 c          498 g          373 t
ORIGIN      
        1 gtagttggtg gtgaaccact tcttcatcgg cagggcggtc acgtcgccct tgtcgccctg
       61 gtagccgcgg gccatggcga agagcgtgtc ctcctggttg tcgaaggaga gacgtgcgta
      121 acgctgcgga atcacgttga gaaggatggc ggtgtccagc atctgatcgt agtagctgaa
      181 gtcgttgctg ggaatgaggt cgatgccggc ggcctgctgc agcttccagt gcttggcgcg
      241 cagttcggcg gcggtggcct tgacggcggc gaggtcgttg gcacccttcc agtagccttc
      301 gatgatcttc ttcagttcgc ggttctgccc gatgcggggg aagccggaga cggaagtcag
      361 agtggacatg aatcctccaa tgtatgcgtt cgtaacccga cttagggtag caaaacatcc
      421 ggattccggc ccttataaat ggccgtagag tgtcgttata gatggaattt atatccgctt
      481 tagtcgaagt ctttcacatt gatgaccgcg agcagttcgc cgctgtgcac ctgccattcg
      541 tcgaacggtt tgttcgatcc catgatcacg atggatgcgg gcgaaagtcc caaattcagc
      601 aggtcgagtc gttcgccgtc ggagtcggcg gaggcgagcc attgggcgct gatcgacaca
      661 gtcggctcgt ggcccacgat catgaacacg cgattcttgg ccttgacgtg ggcgagctcg
      721 tcgaacaccg cctgcactcc cccgtcatac aggctgatgc ggtagtcgac gattggcttg
      781 tcgccaaagg tcttgagcat gcggtccaac gtctggcgcg tgcgggtaac ggacgagcag
      841 gagatttggt cgggcaccag tcccaacgac tcgatgccct tgcccacggc cttcgcctgt
      901 ttcagacctt tgtcggtgag ctcgcgctcc gagtcaccct tgtcgccgaa cggctcagcc
      961 ttggcatgcc tcatgatgat gagaatatgg tcgtatttct tcgccgcttt ggcgacttta
     1021 ctcagcttga tgcccatccg tggctccctt cacgaaccgt ctgcccggtg tggcggtgtg
     1081 gcggtgtggt attgcttcca gtatagaggt gtgtgagcgt tcacaaactc atcgtttggc
     1141 ggttattcgt gcgtattgcc aagttctccc acctgacggt cgaggtcgcg caacacctta
     1201 cgtagtttgt ggtcggagat gtcgcgcagt tccagttcct cgatctgcgc ttcacgctgt
     1261 tcgtcggtga gcgagtcgat gtcggtctgt gtgtccttgg tcacgcgatc gcgctttctg
     1321 agcgcctcga acccggaggc catggcgcgc gagataacaa ggaaaccggt atgaccgatc
     1381 atctggtggt cggggcgtac ggcgaggccc tgggccttcc atgtgcgttc gaacgattcc
     1441 tggatctgcg gctcggtcca gcagccggcc gcgcgcaggg cttcggccat gcggctcatc
     1501 tgcgtggtgg tagttacgta actgaccaac acgccgccgg gggcaatcac gcggtatgcc
     1561 tgctcgagcc tgttccacg
//
LOCUS       DAWBMW010000268         1576 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_14153_length_1576_cov_2.234714, whole genome shotgun sequence.
ACCESSION   DAWBMW010000268 DAWBMW010000000
VERSION     DAWBMW010000268.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14153_length_1576_cov_2.234714"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          328 a          479 c          477 g          292 t
ORIGIN      
        1 gggcaagcag ccgccatacg atccgccgta ccccggcaag tctgacggcg agccgaagga
       61 cgatgtgaac aaaatcaatc agaccacctt cgctgggctg aatgcgtaag gcgggagcga
      121 gaattatgac tacgaacgac atcaccaact ccgctccggt cgtgcgcaag gcggttgacg
      181 gttccgtgat tcctgctgtc aaggccgtgc aggtgcacaa ggcgtttggg ccgctgcatg
      241 tgctcaaggg catcgatcta accgttatgc ccggcactgt gaccgtgatt ctggggccct
      301 ccggttccgg caagtccacg ttcctgcgtc tgatcaacca gctggaaacc ctgaccggcg
      361 gttccatcga ggtggacggc gaactcatcg gctacaagca cgtgaccaaa aacggccagg
      421 acatgctgca gaccctggac gataaggaga tcgccgcaca gcgttctcgc ctcggcatgg
      481 tgttccagcg cttcaacctg ttcccgcaca tgaccgcgct ggaaaacgtg atggaagccc
      541 cgattcacgt gcagcaccag tccaagaaag ccgcccgtga cctggccgtg gccgagcttc
      601 agcgcgtcgg tctgggcgat cgcacggact attacccctc ccagctgtcg ggtggtcagc
      661 agcagcgcgt ggccattgcc cgtgctctgg ccatgaagcc ggagatcatg ctcttcgatg
      721 agccgacctc cgcccttgac cccgaactgg tgggcgaagt gctcaacgtc atgcgttcct
      781 tggccaagga aggcatgacc atggtgtgcg tgacccacga aatcggcttc gcccgcgaag
      841 tcgccgacca gatcgtgttc atggacggcg gcgtagtcgt agaaaagggc ggccccgaca
      901 tcatcgacca ccccaccgag cctaggttta aggacttcct acagcacgtg ctgtaggaag
      961 gctgttatat tacctcagca ctgtcgtgct tcggcgtcgc ctacggggag tccactgggc
     1021 tccccgcttt acggctccgc ctacagcacg tgctgtagga aggctcgttg ttgcagctcc
     1081 aaaagcgtac gctgatgaac tcagcgtacg cttttcacga aggggcatgt tccaatcctg
     1141 caaaggcggc gggttggatt cgctttgcgc aataatcgtc cgctaggctg gtgagccatg
     1201 tgtggaatcg ttggatacgc gggaaatatt gaaacggcat gcggcaagcc tttggaggtc
     1261 tgcctgcaag gcctggaacg tctggaatac cgaggctatg actcggccgg tgtggcgctg
     1321 accgcgcccg gcatggataa ggtggtcgtg cgcaagaagg ccggccgttt gaagaacctg
     1381 gtcgaagaca tcgagcgcaa gccgatgccg ctcgccacgg tgggcatcgg ccacacgcgc
     1441 tgggccacca atggcgagcc gagcgacgtg aatgcccacc cgcacaccag catggacggc
     1501 aaggtggcta tcatccataa cggcatcatc gaaaacgcct ctcagctgcg tctcgacctg
     1561 caagccgaag gctacc
//
LOCUS       DAWBMW010000269         1548 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_14440_length_1548_cov_2.375084, whole genome shotgun sequence.
ACCESSION   DAWBMW010000269 DAWBMW010000000
VERSION     DAWBMW010000269.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14440_length_1548_cov_2.375084"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          250 a          439 c          553 g          306 t
ORIGIN      
        1 gtcggcgccc gggaacttct cgaaagcgaa gcgcggcacc ttggtgacca cgtagtcgat
       61 ggtcggctcg aaggaggccg gcgtggactg ggtgatgtcg ttgcgaatct cgtcgagcgt
      121 gtagccgaga gccagcttgg tggcgatctt ggcgatcggg aagccggtgg ccttggaggc
      181 cagtgcggag gaacgggaga cgcgcgggtt catctcgatg acgatgatgc ggccggtgtc
      241 cggatgaacg gcgaactgga tgttgcagcc gccggtgtcc acgcccacgc cacggatgat
      301 ggcgatgccg atgtcacgca gcttctggta ttcgcggtcg gtcagggtga agcagggggc
      361 cacggtgatg gagtcaccgg tgtgcacgcc gaccgggtcg acgttctcga tcgggcagac
      421 gaccacgacg ttgtcgttgc ggtcgcgcat gagctcgagc tcgaattcct tccagccctc
      481 gatgccctct tcgatcagga cttcgtcggt gggcgagtaa tggatgccgg cgccggcgat
      541 gcggtgcagc tcctcctcgt tgtgggcgat gccggagccc aggccgccca tggtgaagct
      601 cgggcgcacg accagcgggt agccgaactt ctcggcgatc ttgtcgactt cctcgatgct
      661 gtgggcgatg tcggagcggg cggattcggc gccggcctcg tcaacgacct tcttgaacag
      721 ttcacggtcc tcgccacggt cgatggcttc cagggaagca ccgatcagtt cgacgttgta
      781 cttcttcagg acgccggcct cgcccagcgc catggcggcg ttgagcgcgg tctggccgcc
      841 caaggtgggc aggagcgcgt cggggcgttc cttggcgatg atctgctcga ggatcggggt
      901 ggcgatcggc tcgatgtagg tggcgtcagc catctccggg tcggtcatga tggtggccgg
      961 gttggagttg acgaggatga cgcggatgcc ttcctcacga aggacgcggc atgcctgggt
     1021 gcccgagtaa tcgaactccg cagcctgacc gatcacgatc gggccggagc cgatgaccat
     1081 cacggatttg atgtcggtgc gcttcggcat atcacttacc ttccttggag ttgttcttca
     1141 taagttcgca gaaacggtcg aacaggtaag ccgcatcgtg cgggcctgct gcggcctccg
     1201 ggtggtactg cacggagaat gcggggatgt ccacgcactg caggccttcg accacgtcgt
     1261 cgttcagatc gatgtgggag acgaacacct tgccgtactt gccgttttca aacggcgcgt
     1321 cgacctgctt gccgatcggg gcgtcgaccg cgaagccgtg gttgtgggcg gtgacctcga
     1381 ccttgccggt ggtcagatcc ttgaccggct ggttgatgcc gcggtggccg aacttgagct
     1441 tgtaggtgcc gaagccgagt gcgcggccga gcagctggtt gccgaagcag atgccgaaga
     1501 acgggtagcc ggcgtcaagt acctggcgca gcagctcgat ttcgggac
//
LOCUS       DAWBMW010000270         1545 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_14480_length_1545_cov_2.753020, whole genome shotgun sequence.
ACCESSION   DAWBMW010000270 DAWBMW010000000
VERSION     DAWBMW010000270.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14480_length_1545_cov_2.753020"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          323 a          513 c          440 g          269 t
ORIGIN      
        1 tccgagaact gacggcttac acacaaaaac tcgaataatt atgccatgcg gcctggaccg
       61 cccctatgag agcgctcaca accacgaaga cacaccgaaa agggcaagaa ttcgccattt
      121 tcgacgcgcc aatgtagcgt ttacgactcc aacgcacgta gactttgggt catggctgaa
      181 gaaacgaaga tcaaggaaga taccgtcacc gtgcctgtcg cccaaggcga cctcgatgcg
      241 gtgagcaatg ctgaattcta caatccccac ggagttctcg gcgggcatct cggcatcggc
      301 aagcatgccg acaccgccac aatccgcgtg ctgcgtccgc ttgccaagtc cgtgaccatc
      361 ctcacccagg atggcgaata cccgatgacc cacgaataca acggcgtgtt cgtggttacc
      421 gtgccggctt ccggcactaa gaagcagccc acgattcccg actaccgtgt caggaccgag
      481 tgggaaagcg gcgcagtgct cattgaggac gacccatatc gctatatgcc cacagtcggc
      541 gacatggaca cctatctgtt cggcgagggc cgtcacgaga agctctggga gacgcttggc
      601 gcgcatgtgc tgcgctatga cgatccgatg ggcggcgcag acggcacgcc cggcgaacag
      661 gtggtaggca ccgcattctc cgtgtgggcc ccgaacgccc atgccgtgcg cgtagtcggt
      721 aacttcaacg cttgggatgg ccgccgtcat gccatgcgcg agctcggctc ctccggcgtg
      781 tgggagatct tcatccccgg aatcggcgcg ggcgagacct acaagttcca gatcctgaac
      841 gccaattaca cgtgggagat gaaggctgac ccgatggagc gtcagcacga agttccgccg
      901 aacaccgcct ccatcgtcac cgaatccacc tacaagtgga acgatgacgc ctggatgcag
      961 caccgccgca ccaccaaccc gcatgatggc ccggtctcca tctatgaggt gcacgccggc
     1021 agctggaggc agggactgac ctaccgcgac ctggccaagc agctcgtgga ctacgtcaag
     1081 caggaaggct tcacccacgt ggagttcatg ccactggccc agcacccgtt ctccggctcc
     1141 tggggctatc aggtcaccgg ctactatgcg gtcgactccc gtctcggttc ccccgacgac
     1201 ttccgttacc tggtcgacca gttccatcag gccggcatcg gcgtgatcat ggactgggtg
     1261 cccgcccact tcccgaagga tgccttcgcg ctcggccgct tcgacggcac cccgctgtac
     1321 gaggacccgg acccgctgcg cggcgaacac cctgaatggg gcacctatgt gttcaacttc
     1381 ggacgccgcg aagtacgcaa cttcctggtg gccaacgccc tgttctggct tgaggacctg
     1441 cacgtcgatg cactgcgcgt ggatgccgtc agctccatgc tctacctcga ctacagccgc
     1501 gaacccggcc attggcgccc gaacatctac ggcggacgcg agaac
//
LOCUS       DAWBMW010000271         1508 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_14915_length_1508_cov_2.346180, whole genome shotgun sequence.
ACCESSION   DAWBMW010000271 DAWBMW010000000
VERSION     DAWBMW010000271.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14915_length_1508_cov_2.346180"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          298 a          477 c          433 g          300 t
ORIGIN      
        1 ttcgcaggcg tatcgccaga ctgcgccgtc agattcgtga gatggccccg gcgcgtgagg
       61 tcaagcgcgg ttcacgccgt cgcttcggcc tgcccacagt ggccgtggtc ggctacacga
      121 acgccggcaa atcctcgttg accaaccgtc tgaccggttc ggccgaactg gtgggaaacg
      181 cgctgttcgc cacattggac acggccgtgc gccgcgccaa aacccgcgac ggacgcgctt
      241 acgcctacgt ggacacggtc ggcttcgtgc gccgcctgcc cacccagctg gtcgaggcgt
      301 tcaaatccac cctggaagag gtggccgagg ccgacgtgat cctgcatgtg gtcgatggtt
      361 cccaccctga cccgttctct caggtcgacg cggtcaatga cgtgttggcg gacatcgaag
      421 gcacggcctc gattccgcgt attctcgtat tcaacaaggc cgatcaggcg gacgaggcga
      481 ctcgtgaacg actcgccgcg ctgcagccag atgcgttcat cgtctccgcc tataccggtg
      541 agggattgga cgagctgcgt accgcggtcg aaagtctgct gccggtcccg catgtgcatg
      601 tcaacgctct gctgccgtat accgctggct ccctgatctc tcgtgtacgc gaatacggca
      661 aggtagacaa ggtggagtac cgcgatgatg gcatacagct tgaagcggac gttgacgccc
      721 atcttgccgc tcaggtggtc gaacagtcca ttgactaacg tgataaacat cacagtatat
      781 tcgtgagcgc taacaaccgt tgaaaacatt accatacggt tatcaaacag ggtggtgtgc
      841 tggtagcaaa acgtcttagc gggtttatag agtgaagacg ttagttacaa ggcctgccat
      901 tcatcagcag accgcctttg aagagaggtt catccatcat ggcggaaact accgttaagc
      961 ccacgaagct tgctgttatt ggtgccggtg ccgttggctc caccctcgcc ttcgccgctg
     1021 cccagcgtgg catcgctcgc gagatcgtgc ttgaagacat cgccaaggag cgcgtggaag
     1081 ccgaagtgct cgacatgcag catggctcca gcttctaccc gaccgtgtcc atcgacggtt
     1141 ccgacgatcc tgagatctgc cgcgacgccg acatggtcgt catcaccgct ggtccgcgtc
     1201 agaagccggg tcagtctcgt cttgagctcg ttggcgctac cgtcaacatc ctcaaggcca
     1261 tcatgccgaa cctggtcaag gtggctccga acgccatcta catgctcatc accaacccgg
     1321 tcgacatcgc tacccacgtg gctcagaagc tcaccggtct gcccgagaac cagatcttcg
     1381 gttccggcac caacctggac tccgctcgtc tgcgcttcct gattgcccag cagaccggcg
     1441 tcaacgtcaa gaacgtgcac gcctacatcg ccggcgagca cggcgactcc gaagtcccgc
     1501 tgtgggag
//
LOCUS       DAWBMW010000272         1506 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Bifidobacterium longum isolate gSpreadC_ID_1519
            NODE_14941_length_1506_cov_2.039972, whole genome shotgun sequence.
ACCESSION   DAWBMW010000272 DAWBMW010000000
VERSION     DAWBMW010000272.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994623
            Sequence Read Archive: SRR3917689
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bifidobacterium longum (human gut metagenome)
  ORGANISM  Bifidobacterium longum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14941_length_1506_cov_2.039972"
                     /isolate="gSpreadC_ID_1519"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          315 a          469 c          420 g          302 t
ORIGIN      
        1 cccggccaag tggtacgggt ccgaaggcaa cttccagccc taccaggaga acaaggtgac
       61 acgcctcggc gaggatgcgg cccgcccgca ccgcctgcac aagaagcttg tgcgcggctg
      121 agatacgccg gcattagtgc cacaacgcgc tttatacgtc gaaagccgtc cctcaggggc
      181 ggctttcccc ataattgccc catggctgcc cgccccgcat cccataaaat cccacagtcg
      241 cctacggcaa cacaccggca gcgcagcagc aacgtagcgg caatgcgccg cataacccac
      301 cgtttacctt ccaaacagtc tcgttacatt tcgctcccgg aactcttaac ccccaccgga
      361 tagactgcca accataggtt ggaatacgat aaccgaatac tgaacacagc agaacaagaa
      421 tcgaggaacg ccatggctca ggcaaccacc gtaactccat tggaccatct ggtgaaggtg
      481 ctgaagttgg gcgagccttc cgcttaccgc aatcacacat atatcaatgg cgagagtatg
      541 tacttcccca ccggccgcgt ctacggtggc caggtcatcg cccagtcggt gatcgccgcg
      601 tccaaaacag tgggcccttc acgcctgccg cattcggtac atggctattt cattgccgcc
      661 ggcgatattc gtcaagacct gttgtttgac gtcgagaacc ttcgcgacgg ccgctcgttc
      721 tccgctcgtc gggtcaacgt cacccaagcc gaggggtcga ttctcacggc catcgcctct
      781 ttccaggaga ccggccagga gggtgtggaa tttgccgatc cgatgccgga aaacctgccc
      841 gatcccgaaa cgttgacttc cgccaaagaa ctcatgcaac cgttcgccga gaagtcgccg
      901 ttcgccaatt actatgccga gaaatcgccg ttcgatatcc gccacgtgac gccgaccgtg
      961 atgctgcgcg cggacaagga ttccgccgag catgattccg gcaagcagat ggtgtggatg
     1021 aaggccgatg gccacgtcga tgtgccgcag gtcatgcatc gtgcgatgct ggcgcttggc
     1081 tgcgatcagg tgatgatgga gccggtgctg cgccgcgccg gactgtcgat ttccacaccg
     1141 ggcatttcct atgcgtccat cgaccattcg atgtggtggt atcaggatat cgatatcaac
     1201 gaatggcatt tgtatgtgca ggatacgcct atcgccgcgc acggacgcgg actcggcatt
     1261 gccaaagtgt atgcgcagaa tggcgacttg gtggcggcca tcgctcagga agctatggtg
     1321 cgcgtgccgt aggaatagtc gcccctaccg cttgtaggct tgggctatgt cgctacggcg
     1381 cttggcttcc ctgattttcg ggttttcggg gccttcgttc ggcggtagct ggcacaacca
     1441 ttcgccgata atgccgatga tcatgtcggc gatgcacacc acggccgcaa ttgcgcactc
     1501 ggtgac
//
