LOCUS       DAWBSB010000001        29283 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_437_length_29283_cov_3.509717, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000001 DAWBSB010000000
VERSION     DAWBSB010000001.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 29283)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 29283)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..29283
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_437_length_29283_cov_3.509717"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2707..2716
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7664..7673
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8959..8968
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9358..9367
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    19200..19209
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    24765..24774
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5850 a         8320 c         9189 g         5864 t
ORIGIN      
        1 ttgttaaaag catacaaaaa atctgtattt gtgctataat taccccaaac cgaccacagc
       61 cgcggccatg cgcatttcgt gcgctgatgc ttctgtacac ggatgctatt gaaaatcaat
      121 tgaacaccct gtgctccgcg ccgcagaaac gctgccggag ctctgaaaaa ggaggaacag
      181 gctatgacgg acgaaaagat cgcgcgcatc aatgcgctgg ccaaaaagag ccgtacgccc
      241 gaagggctta ccgctgaaga aaaagcggag caggcctcgt tgcgtagaga gtacatcgac
      301 gccatgaagg gctcgctgaa aaaacagttg gacaacacgg tcgttgtgga tgcgaacggc
      361 aaccgtatgc cgctgaaaca aaagcggaaa caataacgtc taatcggcac acagggggaa
      421 aggagggagc cgcatgcgcg accgtgaaaa agaacgcaac atccgcttgc cggagagcta
      481 tgtgcagcag ggcggccccg tgcgtccgcc gcgtccctcc cgcgcccgcc gtgccgcccc
      541 gcgcccgggg ccggagccgc cccgcggcaa aagacagcgg cggcgtcccg tgctgcgcag
      601 ggtgctgctg ggcgtgctgt gcctgttttt ggccgcgtgc ctcagcgtgg ggggctatct
      661 gtatgcttct acgcgcaacg actttttgtg gctggatctg gagcagctgc cgcaccggga
      721 ggcgaccatc ctgtatgcgc aggacaggga aaccggtgaa tgggtggagt atgcccggct
      781 ggaggccacg cagcagaaga tatgggtgcc gctctcggag attcccgagg acatgcagca
      841 cgcatttgtg gccattgagg acaagcattt ttacgagcat cacggcgttt ccctcacgcg
      901 cacggcgtac gcggtgctca acgagatgaa aaaggcgctc accggaacct atttcggcaa
      961 cggcatcaag cagggcgcct ccaccatcga ccagcagctc atcaagaacc tgacgcggga
     1021 cgacgaggcc ggcggcctgg agggctatat gcgcaaggtg cgggagatct ggcgcgctta
     1081 tcgtctggac gcaaagtacg acaaggagac catcctggag gcatacctca acgtcataag
     1141 ctttaccgac aacacggcgg gtgtggaggc cgaaagcatc aagctgttcg gaaaatccgc
     1201 cagggactta tctctggccc agtgcgcctc catcgcctcc atcaccaaaa acccttaccg
     1261 gtacgacccg cgcacccatg aagaagagca tttgagccgc cgcaattaca ttctgtacga
     1321 gatgtggcag cagggctata ttaccgagga acagtacaat gccgcctcgg ccgagcccat
     1381 cggcctttcg ccgggcaatg tgccggtggc ggaaacgcct gtcacgacct atttcaccga
     1441 caaggtgctg acggatgtgt ccgccgctct ttcggagcag tatggcctgg acagcgccga
     1501 gacgaccaat ctgctgtaca acggcggcct gcgcatttac accacggtgg acccggacct
     1561 gcaggcggtg atggaaaacg cgctggagcg cggatacagc agctttttcc ccaccagcgg
     1621 gctgggtgtg gcaacgcagg ccacgaaata caatgaggac ggcaccatcg cccgggatga
     1681 aaacggcgat cccatccggg aggacgtcgt cgagacgccc caggctgcaa tggtgagcgt
     1741 gggctacgac ggcagcctga aagccattgt gggcggcatc ggggaaaaag aggtgagccg
     1801 cgggctgaac cgcggcaccg tgccccgcca ggtgggcagc accatgaagc ccatcggcgc
     1861 gtatgcgctg gcgctggaaa aaaacaagat caactggtcc acgccgcttc tggacgcgcc
     1921 cgtgcggcag ctggaggatg aaaaaacagg cgagctgaag gattggcccg ccaatttcag
     1981 caagacctat gccgaggatg atattctcgt ggtggacgca ctggcacaga gcatcaacac
     2041 catcgccgtg cgcgtgggcg agcaggcagg cgtggggaat atctaccgct ttatgaaaaa
     2101 cagcctgggc gtcaccagtc tgacgccgga ggacaacgac tccggcccca tgatcctggg
     2161 ctcccagacc cacggtattt cgccctatga gctggcgggc gcgtatatga tgctgggcaa
     2221 cgggggctct ttcaccacgt tgcactgtta taccagcgtg cagacgggct acggccgcga
     2281 gatcgccgcg ccggagctga aaacaaagca ggttctggga acggacaccg catatgtgat
     2341 gaaccgcctg ctggcgcagg tgatgcaggg cagcggaacg gctgcgggct accgtgtgcg
     2401 cggcgaaatg gacagtgtgg gtaagacggg tacttcctct gacaaccggg attactggtt
     2461 cgttggcatg acgccctatt atgtaacggc cacctggtat ggctatgatt ccggctttgc
     2521 gctgaacacg gcgtcgggca cccatgcgcc caccacggcc tggaaatatg tgatggagcg
     2581 cgcccagcag ggtttgcccg tgctggagtt ccccgtggac gaaagcgtcg tgacggcaga
     2641 atactgcacg gaatccggcg ggctggccgc gcccggctgc ccgcgcacgg cgacaggcta
     2701 ttacacnnnn nnnnnngagc tgaacgctcc cgcggcgcgc gtctgttttg cacggaaaaa
     2761 ggcggcgcgt tattccagat gaagcacaaa ggggcgcaga ggcgcgggca ccagcccgcg
     2821 gtacacctcc cgcagcagcc cacggagccc cgccgccgtg agcccggccg gcagacggcg
     2881 ggcgatgata gcgtaacgta cgccgtacag ccggcaggcc gcgcccgtca gggagaagcc
     2941 cgcccgttcg taaaaggcga tgcggcgcag gcgcatcgcg ttcaggccgg cgtcatggct
     3001 gtcgggcatc tccacttcgc ccaggatgca gacgccgccc agcacggcgt ccagagcggc
     3061 aaggaacgcg ctgcccacgc cccggccgcg cagctcgggc gttacggcaa aataatccag
     3121 cagcagcacg cggctgtcgt gccggtaaaa aaaggcatag gcggccagcc gcccgttccg
     3181 gtaatatccg taacacaggt aacgcccgcg ctccatcagc ctcagcatca cggccagggg
     3241 cttcagctcg gcaggcggaa acgcctcgtg catgtatgcg tcgtaaatgc cgcgcaaagc
     3301 agactcgttc agttttcgta cagtgctcat ggggacctcc ttgtccggaa aaggttcacg
     3361 ccgcgcggga gcagagcaaa aaagcttatc tcaatggccg caggccaggg acggcctgca
     3421 tggccctcaa aatagcctgc ggcttataga tggaaatcgt cttatatctt cttataaatg
     3481 tttacagcgg agcggtgaag cccttgtaaa ttaaggctct ttgccgtttc tgcttatatc
     3541 ctgctatatc tcgttataag tcctttttgt aagcacgggc gtcaaaaaag cgtcaagtga
     3601 gattgtgatg ttacgctgtt gaaaagtatt ttagaaggat atagagatta agatgccact
     3661 cacattaaaa tctgctcaga aagccactta tagcaaggct atttatgctc ctaccgcgta
     3721 acaagtattc ctcataatta tcgagagcga aagcataact tgttttcgct ctcttgattg
     3781 ctacacaagc aggggcagac gagcttgtca atgactgcga agccgtttac gttatcattg
     3841 acaaaggcgg ctaactcggc tattttcaat tgcattatac gctcacaatc tccccaacaa
     3901 atcaaaattt gtcagttccg tattgcactt ataataatag ggtgaattta attatcgaag
     3961 aaacttaaaa cctggctatt aaagtctttt gcttcttcgt atgtgctatg ccccagtcca
     4021 ttataaattt tcaacttact ttgggctata tgatttgcaa gttcacatga agaagaactt
     4081 cccacaattt tgtcattatc accacctata acaaatgtgg gacattgaat ttttgaaatt
     4141 tcatctaaag cattatgggt aaggcaagca gttgcctgta taataaatct atcgagattt
     4201 ttaggttcag taagtttact aaggattgaa taaaacggac gatatgtttt taatttccgc
     4261 tctgtatagg ttttttctgc tgtatcaata aatatttgct taaagtcttt ttcttttgca
     4321 agtaataacc aattggaaat tacgcttttt gatgtttcat ttggctgaca caaggttaca
     4381 gcaagaacaa gtttttctac caattcagga taatctattg caagatattg tgctatcatt
     4441 ccaccttgag aaactcccag aacaaaagct tttgttatat tcagttgatt catgacatct
     4501 gccaagtcag tagccatatc tcttgtagag cagtcttgtt taagtttgtt tttacggcta
     4561 aacacatata ctttgtagtc tttagcaaat aatttgtaca taagtgcaaa ggtaaccgct
     4621 gaacctttcg ctgtctttga tgcatcaccc aatccgggga tgataacaag acttttattc
     4681 ccatttccaa aagatatata atccatatct gtatcgccta ttttgatatt gcaatttctt
     4741 gcattgtaaa acatatttca gcctccttga ttgaatatta aataaataaa tctcccttac
     4801 aacttcaaat ttgtcgctct gtaaaaaata taaaaataac gaagtcgttt taatagataa
     4861 attatatcct atttctattt ccatttaaat ttcaatctcg gctggacgat atacaaaggg
     4921 gtacccattt gtaaaaaatg cagaggattg gggacacttt ccaacaagca ttttgcaggg
     4981 caaaatatgg aaagggcact cctttcctat acttgctctt cagttcttta ccacaaaaac
     5041 aggaagagga aagaatggat gtgaataaaa ttcgcccgtc aatctgatga agaatatcag
     5101 caacaaatct aattactctc atttcttctt atctcttttt ataagttctc gtagagcatg
     5161 ggcgccaaaa aagcgtcaga cagcatattg aacctgatta catagaagcc cacaaagcca
     5221 tttaaacaca gcttcatgag ggtttgcact attttactcc ttatcgcttg ctacaataat
     5281 tccactattt tttatgatat ttattagtat atcatatttg cacgaaaaat gtgctatact
     5341 gtatttttgg aaaccaacag gggggcaaag tacatgaaac ttacgcataa acatacgctg
     5401 gcggcaagct atctggggta catcacgcag gctgtcgtga acaatctcac gccgctgctg
     5461 ttcgtcgtat ttgagacgtc tctgggcatc tccattggcc gcatcagcct gctcattacc
     5521 gtgaatttcg gcgtgcagat gctggtggat cttttatccg caaaatggat caatgtcatc
     5581 ggaacgcggc gggccatcgt tgccgcccac gcctttgccg ctgccgggct tgtggcgctg
     5641 ggcgttctgc cgtaccgaac ggcggaccca tatgtgggcg tgctgtgtgc ggtttgcctg
     5701 tgcgcggtgg gcggcggcat taccgaggtg ctcatcagcc ccatcgtgga ggcgctgccc
     5761 agcgaagaca aggccggctc gatgagcctg ctgcacgcat tttactgctg gggccatgtg
     5821 ggggtggtgg tgctgtcaac cctgtatttc gcgttggcgg gcaccggacg ctgggcgctg
     5881 ctgcctgtgc tgtgggccgt gctgccgctg tgcaacatgg tgctgttcgc gcgggtgccg
     5941 ctgtgcacgc tggcgggcga cgagcaccac acgccgctgc gcgcactgct gaaaagcaaa
     6001 atgatctggc tgttcctttt gatgatggta tgtgcgggcg cgtccgagca ggggatgtcc
     6061 cagtgggctt ccctgtttgc cgaaacgggc ctgaaggtgc ccaaagcgat gggcgacctg
     6121 ctgggcccct gtgcgtttgc ggtgatgatg ggcctgtccc gtacgctgta cggcatatgg
     6181 ggcgcgcgca tcagcctgaa acgctgtatg gcggccagct gccttttgtg cgtggccggc
     6241 tatgctgcgg cagtgttcgc accctggccg ctgctctcgc tggcgggctg cgcgctgtgc
     6301 ggtttttcgg tgggcattct gtggccgggc acattcagcc tttccatggt acactgtccg
     6361 gagggaggca cggccatgtt tgcgttgttc gccctggcgg gcgacgtggg atgcgcgctg
     6421 ggcccgggcg ttgtaggcgc ggtgtccggc atggcgggcg gcgcgctgaa agcggggctg
     6481 ctggccgcgt gtgcgttccc ggtgctgctg ctgctggcgg tgtcccggct gcgccgccgc
     6541 ccgaaggggg aacagtaaga tggcaagaag cgaaaaagct gtcgtcaccg tactgtgcat
     6601 ggtacatgac gggacgcgcc tgctgctgca aaacagggtg aaagccgact ggcgcggcta
     6661 tacgcttccc ggcggccatg tggaaaaggg cgaatccttt gtgcaggcgg tgacgcggga
     6721 gatgcgtgag gagaccggcc tcaccgtgca tgcgccgcgc ctgtgcggga ttaagcagtt
     6781 ccagacggac gcggacgagc ggtatatcgt tctgctgttc aaaacagacc gtttttccgg
     6841 cacgctgcgt tcatcggagg agggcgagat ggtgtgggtg gagcgggacg cgctggacgc
     6901 ttacccgttg gtagaggatt tccgggagct gctttcggtg ttcgaccgcg acgatctgac
     6961 ggaattccag tacgagcgcg cgcccggcac gcccgcagac tggcgcgtgc gcctgtattg
     7021 aacgcgggat gtcagccctt gtccggctcg ttgagacgca ccatatggat gtggtcctcc
     7081 cacacgccgt tgatgcgcag gtagcggcgc gccagccctt cgggacggta gccgcatttt
     7141 ttcagcaccc gcagggaagc tgtgttgcgg ggcatcacat tcgcctccac ccggtgcagc
     7201 gcggcagggc cgaaggcgaa gcgcgtgcag gcctccacgg cctgcgtcat atagccgcgg
     7261 ccgcacagcg cgccgtccag cttatagccc agaaagcacg actggaacgc tccgcgcacg
     7321 atattgttca gcgcaacgct gccgatgacg cgcccgggga cgtctttcag cgcaagatag
     7381 aagcgcacgc ccgtgccttg cgccgcgcgc tgcacatcgg cggcaagctc ccgcgcgtga
     7441 tgctccgccg tataaaagcc cgcgggccgc tcgggctcga acggtgccag aaacgccgcg
     7501 ttgcgaaggt aatagtccag cacggctggg gccagcccgg gcgcggcggg cagcaaaata
     7561 aggcagcgcg tttccagcgc ggcggggaca agctccataa caagcctcct gttcactgta
     7621 aaaacgacgc gccgcagctt gtcgaaaaag ctttgctttt ccgnnnnnnn nnncgctttg
     7681 tgtttaaaaa aatcagttct ctttccagat gggttccttg agcatccagc ggccgtattc
     7741 gtccttttca aacaggtccc tgttgtagaa cttgtccatg atggcccaga actcggtttc
     7801 ggtgtagccg cagaacgcgc agaagtcacg cacgcacagc gggtccagcg cgccgtcccg
     7861 ctccttgatg atgggaatgg cttcctcccg tgtcatcata ccgtagcgga tgtagcgggc
     7921 cgtgtaatct gtggccgccg catggccgaa cttcgggtat ttcagccagg agtgtaccag
     7981 atacgcgcgg ctgtcgatct gatcgaaatt ttccgcgtga tgggtgcggt cccactcatg
     8041 cgacaggtca tggaagccgt gctccttggc gatctggtaa tttttgtagc tgttccaggg
     8101 cacgaaatag ctcatataga acggatccag cctggccagc tcttcggcgg agggcgcctc
     8161 ggtgaggatg aggtcgttct cgctcacgcc gtagcccagc agctcctcct tggggaaccc
     8221 gacggcaacg ccgttctcga tctggtcctt ggcggaatag gtctctacat cggcgttgcc
     8281 gccgtattca aacgagacgt tttcaccgta gcacaggaaa ggcgtgttaa acttggccgc
     8341 catatgcagc gggaaagtat agatgagcct gtccacatac caggtgggct tgccgtattt
     8401 ttcaaaaaag gtgcgcatca gggttttctg cgccttgata ttgggcttac aggagatgat
     8461 ggtgcagccg aactcctcgc tgatgttttt caggttgtgc ttgcccgcct ccgtcatggg
     8521 gaagttgtcc tctacgctgt acaggatggg gttcatgcgc atgacttcct tgagcatata
     8581 cacctggaaa tggctgtcct tgccgccgga gacggccacg gcgcagtcat atccgccggg
     8641 gccgttgcag ccgcggtatt tatcgcacat agcctcaaat tccttccagc gcgcgtccca
     8701 gtccacatcc ttgcggtggg cgtaatggtt gcacgcgctg cacacgccgt tttcgtcgaa
     8761 cgtaatgccg gggcgcgtgt cgggcatggt gcatttttta caatatttca tagcttttct
     8821 cctttacaag aaaccgcgcg gaagaaagcc gcgcatcgta agcactctta ttgtacccca
     8881 ttttgcgccc gcgcacaagg cgcagcggcg catttgcgca caaaagaggc gctctgccgg
     8941 gcgggggaga gtgccgggnn nnnnnnnncg cgcggggggg aagccgaacc gccatccggc
     9001 tggacggcgc gcgccgtttt gacgccgggg catcagccca caaagcgcgc gatcttttcc
     9061 tccatggcgc gcgcctcctc tttggaaatg tgcgccttgt cgatataacc gtaaatgcgt
     9121 gaagccatat ctgcgaagcc gggggcgacc ccggcaaggc gggccgggtc gaatttcacc
     9181 gcgtacatca tatgcacacc gatgagcgcg cagaatttga gcaaattgaa atgcggccgg
     9241 aagggcgggc tgaagggctg gtcccgcgga gggtttttgt aaacgtcctc cagcaggtcc
     9301 gccatgcgca cataggcggg cgtatcgctc ctgtcaaagt agccttcgat gacctctnnn
     9361 nnnnnnnttg gcaatgggca agggcgcact gtacgccgcg tccgccgctg cggcaaaggc
     9421 ggcgtaatcc gtgatgtaat ccgccccctt ataaatgacg gggtcgtact cgtgctcaat
     9481 gggaacgggc cgcaggatgc ggcagctttt gcccgcgaag tatacctccg caatggccgt
     9541 gctcatccag atgaagatct ggtccgccgc cacgatccat tgcttcacgc tgtccgcgaa
     9601 gatcacgcgg aaattgggcc gcttttgggc cagcgcctcc agcgcggggc tgttccactc
     9661 gctggggtgg cggcggtaga cgagctgcac ttcgggatgc ccggccagat attggtcgaa
     9721 ccaggccagc gtctgttcca tactggtgcg gttggtacag gcaaagccgg tgaaatcctc
     9781 gcccgccatg tcggagagct cctttacttc ctgctccgtc atgctggcat agccgaagct
     9841 ggagatgtac agcatcagtt ttttgtccgg gtccaatccg tgctcccggc acagcgcagc
     9901 cttatccttg aaatatcccc ggaactcggg ccgcaggaaa tccatcatca ccgcgcctgt
     9961 cacggggcag tttttttcct gcatgccgtg ggccacaagg cgctgctgcg tgcgtttgcc
    10021 ccagcaggtc tgcacgcatt ttttggcgtt gccgccgaag ttgaaccacg cgccttcctc
    10081 ctgcgtgtcg ctgagcatct gctcccagtg caggttcacc actttgttgc acacgccgat
    10141 gttgttgtac acatggctgt tgatggcctc gtcgtcgtac atgcagctgg acaccagaac
    10201 cttcggcttt ttcagggtga agtatttgag ctttttccgg tccagcagct ggcggagctc
    10261 cacggtatag ccgcgccggg ccagctccgc cttcaaaagc aggatgctct cgtactcccg
    10321 caccacatgc tcatacagaa tcagaaaatc cagtgccatg gcttacgcct cctttgcgcc
    10381 gaacagcgca cgtgtaaatt cgggcaggcc ccgttccaca tccaggtgga acccgtcgta
    10441 gtattgctcc tccgcgtaaa cggcttccgg ctctacctca aaatcccgca cggccgcgcc
    10501 ggtgtcagca agcgcctgcc gtatggtgcg catgggctcg tccaggccca gcggcgcaac
    10561 gagatactgc cgcagcgcat cgtcgatggg gggcagtaca aaggtcagtt caatgccgtt
    10621 ttccgcacaa taagccgcca gctcctccag acgccgcagg ttttcccagt tcagggaata
    10681 ggagctgtcg gcgggcgttg cggccagcat cgcgtccagc aaagcgcggg gattggtgca
    10741 gcgatcccgc gctgcgcccc aggcgtcgat ataaggctct gtttcaatcg cgggcagtgt
    10801 gcccggcttt gcgcagtttt ggtacagcgt ggcggcatag gcgatcaggt tgcggcgtac
    10861 gggcagcgcg ttgccgtcct cgtccacaga atcgatcgcc tcccaagcgc ctgcgtcccg
    10921 ggtcgcaccc gtctgcacgc cctgcaggcg cagcagaagc tcacccagca tatcggcgtt
    10981 gtaatcgaaa ttgagcatat aggcaacggg gtttttcacc acggtgccga tggcctccat
    11041 gcggttgcgg gaatcgccgg cacgcagggt gtaaaagctg acgccgaaat agcaggtgtt
    11101 caggctgggg ttgttttcgg cggcgaggta gaacaggtcg atgctctcgt tcagcgaagc
    11161 gccgccgaag gcgaggttgc tccagccgcg gccggaggcc tcggccacgg cattcatgtc
    11221 gaaatgggcc atgcgtgagt cgcccaggat gatggcgtcc tcgggcgcgg acagataagc
    11281 ccgcacgcgc gtgatgacgg agtcttcgct ggaagcaccg cctttgacgc cgaaataatc
    11341 atagggctca aaggcgaaaa acagggcaaa ataaaccagg aacggcgcgg caagcagcgc
    11401 caggcggggc aaaatttttt tcatgcggcg cgcctcctta aaaattgttg tacaggaaat
    11461 tcgccgccgt ggcgaagtat ccgttgttca tcaaaaagcc cgcgaaaatg aacgcggcca
    11521 gcgcgtagta cagcagccag cggaaaataa gcggcaggct cagcatccgt gcggaaagat
    11581 ccccgcccgg cgctttgaag cggcgggcac agtccagcag cacgcctccc gccagtgcca
    11641 aaagcacgaa cgccaggaat gcagcgctca aaagagattt tgcattgaag gaggccgcta
    11701 tggcgcggat gtccgccagc aggcctgtca gtgaaacgtt ccgaaactgg gccgtcagca
    11761 catacagcgc gtcgtgtacc gaagcggcgc ggaaaaatac ccaagcggcg tttaccagcg
    11821 caaaggtgca caggcactgc cacgcacagg caaggcggcc ggtgcggctg acatgcagcg
    11881 ctgcgtaaac gcgtccgcgc agcctgtccg tcgcgtcccc cgcgatctgg tagacgccgt
    11941 gcagcgcgcc ccagatgaga tatgtcagcc ccacgccgtg ccagacgccg ctgacgagga
    12001 atgtgagcag caggttcaga tacttgcgcg ccttgccctt gcggtttccg cccagaggga
    12061 tgtaaacata gtctttgagc caggaggaaa acgagatatg ccaccggccc cagaactctc
    12121 ggatggaacg cgaaaaatag ggcgtgtcga agttcgtcgt cagctgaagg cccagcagct
    12181 cggcagcgcc cagcgccatg agcgagtagc cggaaaaatc aaaatacagc tgcagcccgt
    12241 agcacagcgc gccgaaggtg agcgtcaggc ctgtatacgc cgttacgtcg ccgtaaacag
    12301 cgttggcaaa caccgccaga aggtcggcca cggccacctt ggagcacagc cccagcgcaa
    12361 aggtgaccag cgcgtgggcc gcgcggtccg tacgcagggc gcggggcgcg cgcagctgcg
    12421 gcagcagttt gtccccgcgc atgatggggc cgctggagat gagcgggaaa aagcttacga
    12481 acagcgcgaa attacaggcg ctcggctcgg ccgcgtattt tttgcggtat acatccacgg
    12541 cgtagccaac ggactgcacc gtgaaaaagc tgatgcccag cggcaggatg acggaaaaag
    12601 cgctgccgcc cgctttactg gcgaggccca gacgggacaa tgcctgccaa agccctgcgc
    12661 tgacggccgc gccgtggagc agcagagagg cgcctttcag cagcaccaga aggccgaggt
    12721 ttacgcccag cgcggcccag agccgccgtt tgcgggagag accctcggcg gcgtccatgc
    12781 ggcgcaccag cgcccacgtg gccagggaat ccagaaccag cacgggcagc catgcagggg
    12841 tattgaagca ataaaacgca tagctgacgg ccagcagcgc ggcgttctgc cagcgcctgg
    12901 ggacaagccg gcatacaagc accgccagcg ggaagaatgc aaagaacgaa acggagttga
    12961 atgtcatagc gcacatccta tatcgacaga gtatggaccg cgccgcagag cgcggcccgg
    13021 cgcgcgggaa tccttgttac attataaagg gggacaggcg ttttgtaaag gaaggggcct
    13081 gccggctgcg cggggcaagg cggaaggcac ggcggcgctt atgcccggcc gacaaaaaag
    13141 cctcctttta ttgctattta gcccatttta tattatactt aactggtatc ggtgtgcaaa
    13201 agaaacagcg gttttcgggc acgcggcagc gcatcggctg cgctatccgc gctgccttct
    13261 gtcgggaagg caacgctgtc cgccccgccg ctgccccgcc gcgcggctga aagcttcgcg
    13321 ggccccggcc gggcgctgct gcttttatac acggataccg tgatatacta tacccgtaag
    13381 caaaaaacgg gcggcgcgca ccagaccgga atttgcggcg cagcccggca gaaaaggagt
    13441 tttccatatg gcgcatactg ttatggtgac cggtgcagac ggctttatcg gcagccacct
    13501 cacggaagaa ctggtgaaaa aaggcgaaaa agtgaaagcc ttctgctatt ataattcgtt
    13561 taattcctgt ggatggatcg attccctcgc gcccgagatc cgcagtgaga ttgagatttt
    13621 catgggcgat atccgggacc ccaacggcgt gcgcaccgcc atgcagggac aggagcgcgt
    13681 gttccatctg gccgcgctca ttgccatccc gttcagctac cactccccgg acagctatgt
    13741 ggacaccaac atcaaaggga cgctgaatgt cctgcaggca gcacgggacc tgggcaccca
    13801 gcgcctgatg gtgacttcca ccagtgaggt gtacggcacg gcccagtatg tgcccatcga
    13861 cgaaaagcac ccgtatcagg ggcagagccc gtactctgcc accaagatcg gcgcggaccg
    13921 gttggccgag agcttctacc gcagctttga cgtgccgctt tccatcgtgc ggccgttcaa
    13981 cacttacggc ccgcgccagt cggcccgtgc cgttatcccc accatcatca cccagcttct
    14041 ggcgggccgc acggagatcc gtttaggcag cctggcgccc acgcgggatt tcaattacgt
    14101 caaggatacg gccgccggct tcatggccat tgcagactgc gacgccgccg tcggacagga
    14161 gatcaacatc gccactggcc gcgagatatc gatcggcgat ctggcggatg aactgatccg
    14221 gcagatcaat cccgaagcca gggtggtgtg tgaggctgag cgcctgcgcc ccggcaaaag
    14281 cgaggtggaa cgccttttgg gcgacgcctc caagctgcgc gccctgacgg gctggcagcc
    14341 ggcctacacc tttgagcagg gccttgccca gaccatcgaa tggctgcgcg gaaatatgga
    14401 cggctataaa gtggatgcat acaatttgta atatatcgac aaaatattac aaatctgccg
    14461 cctgcaggac acaacgtgcg ggcggcgaat ggagtattta tggcggaact tacaaaaaaa
    14521 tgcaaacggc tttggacaaa gcccggcatc cgctggcttt gcggatattt ctttgtgctg
    14581 ctgtgcggcg tggggctggt atattactgg cgtgtggcgt actcgttggg cgcgggcatg
    14641 cccggctttg ccgcagcgac gacactcatc atctgcggcg gcttttccgc cgtgtatctt
    14701 ctggtgtttc tggccgcgca tttcctcaaa cagaacctgg ctctgaaaac cgctgtgctg
    14761 gtttttctgg ccgggctctg tttcgtgttt gccaacccgc ccatgcaggc tccggacgag
    14821 acgatgcatt tcctgcgggc ttatgccgtg ggcagcggac agttcctgtt cgatgaaaaa
    14881 cagcagttcc caaacgatgt gaatctgctg gtgcgggatt tccccggggc gtatatcaac
    14941 ggcgtcagcg gtgaaaaact ggccccggac gacccgacca tcgcggacgc ctttgcacgg
    15001 tacagcgcgg acaaagctga gggggcggtt gcgccgggcg cgttcacccc ggtgcagcag
    15061 ctggtgccgt acttccccca ggcgctggga gtggccgtgg gacggctgtt cggcgcggac
    15121 gcgctggcct gtatgtattt ggcgcgcgcg gcgaacctgc tgtgctatgc cgtattgtgc
    15181 ggttacgcgg ctgcggtcat ccggcgcttt tcggccgtgc tgatcgcgct ggccatttct
    15241 ccggtgtcgc tgtttatggc cggctcgtgc agctcggacg ggctgttcat gggcctgaca
    15301 tggatattta tcgcgctgtg cctttcggac gttgtgacga aaaagaggct tgcggtgctg
    15361 gcgctctgct ttggcttgac gttccacgca aaatacacca cgctggcgct gctgccgctg
    15421 ttgctgctgc tgccgttcga gcagaagccc cgcaagggaa aaaaggcgct ttcggccgcc
    15481 gcgcagcgct ggggcgtgct ggcggtatgc ctggccgcgg gcgttatcgt atatgcgctg
    15541 ctgacaggat atacggcgct ggccagcaat tatggccgtg tgccttatgc ggacaccggc
    15601 gttgacccgg gccggcagct cgcgttcgtt ttttccaatc tgccgcgcta cctggcggtc
    15661 ctgctctatt ccgtctaccg tgacaaggca aacctcttta ccattgggaa ttttggatgg
    15721 atggacatga cggtgccgtt tgtcagctac tttgcgccgc tttttctgct gtttgcatcg
    15781 ggcgtatcgg cgctggaggg cgcgcgggaa aagctgcgca ccgcctgggt gtttctggcg
    15841 acggccgtgc tgatgtacgg cttcacctat acgggcatgt acctcaccag cacgcccgtc
    15901 agcctgccgg agatcaacgg cgtgcagacg cgctatctgc tgggggcgct gtttgcgctg
    15961 ttcgcgctgg catccatgct gatgggccgc acgatggccc tgcagaacct gcgcccgggc
    16021 ctgccgcaga aaacgccgcc cgcgtggcgt gtgctgcatt acggtttttg cttcgcgctc
    16081 gtcagcgcgc tgctgctgtt ccaggcgtat tacatcgggc cgtgacgctg tgcggcggct
    16141 ccgtatcgct ttaaaggagg accctgcgtt tgatcgcttt tgttgaaatg accgttacct
    16201 tcgcggcgct cacggcgctg tgcctgttcc tcaacacaaa aacgcgtctg gccgcgggcc
    16261 ttacgccgct gtttgttttg tgcggcacga tggtctggta cagcacgctg ggcagcgtgc
    16321 atatgctggt gcctgcgggc attgtctggt ttggcgcggc ggcggccgcg tgcgtctggc
    16381 tgtggcgcaa gcacaaggac atccgctggc gggagttttt ttcgcccgcc atggtatatt
    16441 tcctgctggc gtcgctggcc gttatcgtgc tgtttgccgt gcgccggcct atttttaacg
    16501 agtgggacga gttcagcttc tggggcatcg cgcccaaggt cgtcaagacc gagaatcagc
    16561 tgtataccta taacccgggt gagatgcgcg tgagcacctt tgtgccgggc atcatcatgc
    16621 tggattatgc gttccagttc ctgggcagcg tatttgtgcc ctggaaggtg tacgcggcgt
    16681 acgacatttt gttttttgca gtattcgcgg ccgccatttc catgctgggc cgcaggcact
    16741 ggcacattgc cgtacccgcc gccgcggtgc ttactctggt gccatacatg gtttccgtgt
    16801 atcagcgcgg catctatgtg cagacgacgt atatgaacgc ctattccgac attcccatgg
    16861 gcctgctgtt cggcgcgggg ctggtgctgt attttgcgcc gcgaaaaaaa acacccatac
    16921 tgatgacggg tgcggtgctg gccgttacgg cgtcctgcct ttccaaggac atgggcttcg
    16981 cgctgtgcct tattgcggcc gccatcatct gctttgacct gctgttcgtc cagaaggagg
    17041 acgtgccgtt cttccggctg aagggccttg gcggaaagct gtgctggtgc gcctcgcttg
    17101 tgggcgcccc gctggccgcg ttcttcggct gggccgcgca catgagcgtt gtgctgggct
    17161 ccaaccggtt cgacatcggc ggctcggccg atatgggcat ggtacagatg gtgacgacgg
    17221 gcattgccga gctgctgggc atcggccgca cccagaaatt taccgatatc atggagctga
    17281 tgaaaagcgc gtttttcaac acacggctca cgatgttcag cgttggcgcg ccggacagca
    17341 cgctggggcg tattttcaac ggctcgggct tcatcaccgt gctgctgatt ttgagcattc
    17401 tgctggcggc attcctgctg ggcgacaagc gcatgcgcgt gcgcacggcc tggacggcgc
    17461 tgtggagcac gctgggcttt gcggcgttct atatcttcac gggtttcacc tatgtgtatg
    17521 tgttcaagga agagctggcc tatgggctgg gcgattacaa ccgctatatt tatccctatt
    17581 atgcgggctg gctggtgttt gccgtaacga tgctgtgcgc ttcgctgaaa aatgcaaagc
    17641 ccggtagtct tggcacgctg ttcctgctgg cgctgtgcgg aggctgcatt tggcgtgcgg
    17701 atgcatattt gcagccccag cttacggttc tggattaccc ggacagccat tacgcgggcc
    17761 gccgtttgca ggtggaacag gttgaggccg cgaagcacta cctgacgcgg gacgacaaag
    17821 tgttcatcgt gtccatgacg cagcagggcg tgggctggtt ccagctttat tatgaatttt
    17881 atcccgatgt ggccgtagat tacagcttcg gcgcagggga ggaattcagc ccggacatcg
    17941 tgcgccgggc cgacgcgatg ccggggttct ttacggaaga acaggtggac tacttcacca
    18001 gccagccgtt tacacccgcg gtctggtgcg attaccttga ggccagcgga tgcacggcca
    18061 tctatatgga tgaatgggac gcggcctttg cggaaaatta cggcgcgctg tttgccgacg
    18121 gcctgaaaag cggcgccacg ctgtaccgcg tagagggcgc gggggcggat atgcactttg
    18181 tgccgctgaa cggagaggag gcggcgtcgt gaagcgtgtg aaggcttttt ggaacagcat
    18241 ctggaaaaac gacaggcggc tggtggcgct ttgctatgcg gtgtttctgg caggcagcat
    18301 actggcgtct ctgtacgggt ttgccgagga cgcataccag cgtgcccggg gcaacgtggc
    18361 gcagacggag atttccggcg cgcaggaaga catatttgct ctgacggacc tggaacagga
    18421 gggcgattta tacacctcta ccagtgtgga cccgcgcatg gagctggacc ttgccgccgt
    18481 atcgcccacg ggcgtgcccg cttatgtgcg gcgcgttacg gttcgggtga cgtttttgaa
    18541 tatggacccc ggcgagctga gcgtgttcta caaaccgcgg gcggacatgg aggaatacga
    18601 cgcgacctac cgggtgtggg cgcataagga agccgaagac ggggtatata cctttacgct
    18661 gccgcggggt gcgctgtatg gtttacggct ggaccccggc atttacagcg gcatgcaatt
    18721 ccggctggag agcgtcatca tcaatgaacc gcgcggcttt ttcgaatggt tcctgcccac
    18781 gcgtccctgg ctgctgtgcc tggccgtcgt accgctgctg acggcctccg tgctgaaata
    18841 tctggcgctg gccgcggctg cgctgggcgc aaggcgcgcg ggcggcaaaa cctgaggcgg
    18901 ccgcggcaag cgataccgcg tgcgcgcggg cggcgcagca gccgcccgtc ccgcccgcta
    18961 tttttataaa ggaaagaagc gaagtatatg agccagcaat ttatcccgct gtccgtaccg
    19021 aatttttccg gcaacgaaaa gcagtatgta aacgaagccg ttatcagcga gtgggtgtcc
    19081 acgggtggca gcaaggtgtc cgagtttgag gaaaagatcg ccgaatacac ccacatgccc
    19141 cgggccgtgg cctgcaacag tggtacctca ggcctgcatc tggccatgct catggcgggn
    19201 nnnnnnnnnc atggcgggcg tgggccgcgg cgatgaggtg ctcgcgcccg cgctcacgtt
    19261 tattgccgca gtgaatccca tccgctacgc gggcgcggag cccgtgttca tcggctgcgg
    19321 cgagagcctg tgcctgtgcc cggcgctggc cggggagtgg cttgccgcca acaccgaaca
    19381 gcgggacggc aaatgcgtga acaggcacgc gggcgcgcac gtgaaagcgc ttctggcggt
    19441 gcatgtgttc ggcaatatgg ccaatatgcc cgggctggtg gagctgtgcc ggaagtacgg
    19501 cctggtcctc attgaggatt ccaccgaggc gctgggcact tattacaccg agggaccgta
    19561 caggggcaaa atggccggca ccatcggcga catcggcgtg tacagcttca acggcaataa
    19621 gatcatcacc acgggcgcgg gcggcatggt ggtgtccaac cacgcggact ggctggagcg
    19681 ggcgaagcat ctttccaccc aggcgaaagc ggacgagctg caattcgtgc atgacgaggt
    19741 aggttacaat taccgcctga cgaacctgca ggctgcgctg ggcctggcac agctggagca
    19801 gctggaaggc tttattgccc acaaaaacga aatgtacgat ttttataaag agcggctgga
    19861 cggaaaaaac ggataccgca ttctgccttt ccgggacggc gtgcgctcca acaagtggtt
    19921 ttacagcctg tatctggagg acgcgcggca cacgcgggac ggcgttatcg ccgcgctcaa
    19981 ggcgcagaac atccaaacgc gcccggtgtg ggcgctcatc cacgagcagg cggactacgg
    20041 ccgcaacgag acctacggcc ttgcaatggc cgaggatttc cgcgcgaaga tcgtcaacct
    20101 gccgtgctcc acgaacctga cgcgggagga tgctcagcgc gtggtggacg cgctgctggc
    20161 gctgtaaaag catggcggaa ggggaaaaaa agatgcgcgt ggacgacctt ttcatcaccg
    20221 aggacatttc ggtgctggat gcaatgcgca agctggacga aacgggcctg ggcgtgctgt
    20281 tcgtcgcgcc ggagggtgtg ctgcgcgccg ttgttacgga cggcgatatc cgccgctaca
    20341 tcctgaagag cctgccgctg gaagggccgg tgcgggaggc ggcgaattac agcccatacg
    20401 cgctgcccat ggaggcgcgg gcgcgggcca agaccgcgct gctggaacac ggcatcggcg
    20461 cgctgccgct gctggacaag cggggcaggg tgcaggacgt ggtgttcgcg ggcgggctgg
    20521 acatcgacac acataaaaaa gcatgcgcgc ccgtggtcat catggcgggc ggtttgggca
    20581 cgcgtctgta tccctacacc aaaatactgc ccaagcccct gatccccatt ggggaaatgc
    20641 ccattgtgga gcatatcatc caccgctttg cggatgtggg ctgcagcgcg tttacgatga
    20701 tcgtaaacta caaaaagagc atgatcaaga gctatttcaa cgatctgcaa aaggattaca
    20761 ccgtggatta tgtggacgag gacacgcctc tgggcaccgg gggcggcctg agcctgctga
    20821 agggaaaagt tacgcagacg ttttttctga cgaattgcga catcctcatc gacgcggact
    20881 tcggggatat ctaccagtat cataaggaaa agggcaacgt catcaccatg gtgtgcgcgg
    20941 tgaagcattt caccatcccg tacggcgtgg tggagctggg cggcgacggc gacatcagcg
    21001 gcatcactga aaagccggag atgaatttcc tcacgaacac gggtgtgtat gtggtggagc
    21061 cgtcggtcat cgacggcttt gcgtacaacg agccgatccc ttttacagat atcatcgcgg
    21121 atgtgcgcgc cgcgggcggg cgcgtgggcg tgtatcccgt gagcgagaac agctggatgg
    21181 atatgggcca gctggaggag ctggacaaca tgcgccgccg tttggagatg cagtaggccg
    21241 ccgcggcata acacaggcag ccgcgccggg caaggcccgg aaaaacgcag cggcccggcg
    21301 ggaaccgggc ggacaggggg aaattatatg tcagtgctca tcatcgcgga ggcgggcgtg
    21361 aaccacaacg gcagccttgc gcgcgcaaaa aaaatggccc ttgcggcaaa aactgcaggg
    21421 gcggatatcg taaaatacca gacggcggtg ccggagctgg tcgtgagcaa atttgccgaa
    21481 aaggccgaat accagaaggc gcagacgggc gccggcgaaa gccagctgga gatggtgcgc
    21541 cgcatccact tcggctttga cggccaccgg gagctgaaag cttactgcga ggagatcggc
    21601 atcgaatacc tctccacgcc ctttgatctg gacagcatcg atttcctggc gtcgctggag
    21661 ctgcccgtat acaagatccc ttccggagag atcacgaacc tgccctatct ggaacggatc
    21721 gccgcgctga aaaaaccggt catcctctcc acaggcatga gtacgctggc ggaaattgag
    21781 gacgcgatgt ccgtgctgga ggacggcggc gtggaggata ttacgctgct gcactgcaat
    21841 accgaatatc ccacgccatt cgcggacgcg aacctgcgcg ccatgcagga cctggcgtcc
    21901 catttcggct gcgcggtggg gtattccgac cacacgctgg gctgggaggc cgacatcgcg
    21961 gccgtggcgc ttggcgcgtg cgtcattgaa aagcacttta ccctggacaa aacgctggaa
    22021 gggccggacc acaaagccag cctggacccg gcggagcttg cggccatggt gcgcgcggtg
    22081 cgcaatacgg agcaggcttt gggcgacggc cgcaagcatg tgacgtccag cgaggcgaaa
    22141 aacaaggcca ttgcccgcaa gagcatcgtg gcccggcgcg ccatcgccgc gggcgaggcg
    22201 ttcacggagg aaaacctcac caccaagcgt ccgggagacg gcgtttcgcc catgcgctgg
    22261 cacgaggttc tgggccaaac ggccaaacgc gcgttcagcg aggacgagaa gatcgagctg
    22321 tgagcgaaaa gagagggctc cgccgacgag gcgcggaagc ttttatttta taaaccggga
    22381 gggattcaga tgacgaaaaa aagcctgtgc gtcgtcaccg gcacgcgcgc cgaatacggc
    22441 cttcttcggc ctgtgctgca aaagctgctg caaagcgccg tcatcgagcc gcgccttgtg
    22501 gtgacgggcg cgcatcttgc gccggagttc ggcaacaccg tcagcgagat cgaagccgac
    22561 ggcatgccca tcgcggcgcg catccccatc ctgaagttcg gcaccggctc tccgctggct
    22621 actgcgcgca ccgtcagcta tacgatggag cgctttaccg attatttcag ccaaaaccgt
    22681 cccgacgcgg tgcttgtgct gggggaccgg tatgagatat ttgccgcggg cgcggcggcc
    22741 gcgctgctgg agatcccgct ggcgcacatc agcggcggcg acgtgacggc gggcgcggcg
    22801 gacgattggt tccgccactg cctgacgaag atggcgtccc tgcactttcc gtcctgtgaa
    22861 gtttacgccc ggaggctcat ccgcatgggg gaggaaccgg ccgcggtgga gaatgtgggc
    22921 ggcctggggg acgagaacct gcgcagcctg ccgcttttgt cccgcgcggc gctggccgaa
    22981 agcatcggct tcgggctgga tcgcccgtat gcgctggtga cctaccaccc cgaaacagcg
    23041 ggcggcgcgt ccccggcggc gcagttcgac gcattgtgcg ccgcgctgga gcgctgcggg
    23101 ctggccgccg tctttaccaa agccaacgcg gatgcgggcg gcgccgacat caacgcgcgg
    23161 atcgacgcgg tgtgcgcggc gcgccccgga cagtatatcg cctttaccag catgggcgtg
    23221 ctgcggtacc tttcggccat gaaatactgc gccgtggtgg cgggcaactc gtcctccggc
    23281 gtagtggaaa cgccctcgct gggcgtgccc tgcgtcaacg tgggcgacag gcagaagggg
    23341 cggctgctca gcgccaatat catctgctgc gcggcggacg aggcgtctgt gtatgcggcg
    23401 ctgcaaaagg cgctttcgcc cgcctttgcc gcgcaggccc ggcaagcgaa aagcccttac
    23461 aacggcggcg acaccagcgg gcgcatcgtg cggcggctgg aggcgttcgt gcaaagcccg
    23521 tcgcttggaa agcccaagct gttttacgac ggccccgtgc cggggtggga aaaggaggac
    23581 agccgatgag cggattggcg gattccttcc gcgagatgtg gaaaacggcg ctgacggcgc
    23641 tgggacagct gaacaccttg gtgcccgctt actgggacgg cgcgctggat acagcactgc
    23701 gcgtggtgcg cacggcgctg cctctgtttg cggcggtgct gctggtgcag cttgtgctgc
    23761 tggttttcgc gcggcgaaag cgcccgcggc gcatcctcgt tttcgccggg acgctggccg
    23821 tggtgctggg actgtgcgtt tgggcctgtt ttcccgcatg gacggcgggg gcataaacgg
    23881 aacgccccga ggcggcgggc cggtaaaagg gtccatgcgg gggttgctcc cggaagcgat
    23941 acaccgatat tgaaaagagg gctgtgcact gcgcggcacg gggaggaaat gatatggaac
    24001 ttttggtcgt aggtctgggc agcatgggca aacgccgtgc gcgcctcatc cggggcgtgg
    24061 agccgtcggc ggcgctggcg ggcgtggatt tcgcccccgc gcgcagggaa gaagcgcggg
    24121 ctttgggcat cgagccgtat gagagcatcg cggcggcggt ggcagcgcgg cactttgacg
    24181 cggcgttcgt gtgtaccgcg ccccttaccc acgcgggcat catcggcgag ctgctggacg
    24241 cgggcctgcc tgtattcacc gagctgaacc tggtggacga cggctacgac gcgctcattg
    24301 caaaagccgc gcagaagggc ctgccgctgt ttttgagcag caccatgctc taccgcgccg
    24361 aaacgcagta catcaagcgc cgggtggcgg cgtttgcaaa gcccgtcaat tacatctacc
    24421 acatcgggca gtatctgccg gactggcacc catgggaaaa ttataaaaac ttttttgtgg
    24481 gcgacaggcg cacggggggc gtgcgggaga tcttcggcat cgatctgccc tggctgctgg
    24541 acgctttcgg tccggtgaag cacgtctatg cggaaaagga caagctcagc ggtctggaaa
    24601 tcgattatcc ggacagctat ttcgtcacgc tgcgccacga gaacgggacc aagggccttt
    24661 tggcggcgga tgtcgtcagc cccaaagccg tgcggaactt tgaggtattc ggcgaagggc
    24721 tgcacctgtt ctgggagggc aaccccaggg cgctgtacag ctacnnnnnn nnnnagctac
    24781 aacgccgaaa ccagggagaa ggaatttgtg gacacctacg gcagcttcga gcatgatgcg
    24841 cggtacagcg acaatattgt ggagaacgct tatgtggatg aaataacgaa ctttttcggc
    24901 gtgctggccg ggcgggaaca gccccggtgg tcctttgaaa aggacaaaga ggccatcgcg
    24961 ctcatcgaac gcatcgaggg ggcgtaaggc atggacgtat tgtttgtagg gctgggcagc
    25021 atcggcacgc ggcatttgaa aaacctgcac gcggtggcgg cgcaaaagga cattccggtg
    25081 cgcgcgtggg cgcttcgctc ctcggcccgt gcgcttccgg aggaaacgcg cgcgctgctg
    25141 gcgggcgaat tcacatccct gcccgaacac gcccgctatg acgcggcgtt catcacgaac
    25201 ccgacgcacc tgcatttcgg gatgttacaa aatctgcggg gcaaagcgga tactcttttt
    25261 atagaaaagc cgatctttga acgcacggac cgggcccttg cggactgcct ggccccgggg
    25321 cagaaggcct atgtggccgc ccccatgcgc tggtgcggca cgatgctggc cctcaagaag
    25381 gcgctgccgg cgctgtcggt gtattcggcc cgggtgctgt gctcgtccta cctgcccggc
    25441 tggaggcccg gcgtggatta ccgcaccgtg tattccgccc acaaggacat gggcggcggc
    25501 gtcaccatcg acctcatcca tgaatgggat tatctggtgg agcttttcgg caagccggaa
    25561 aagatgtata attttaaagg gacgtacagc catctggaga tcgactcgga cgacctttcc
    25621 gtctatatag cccaataccc cacgctgctg tgcgaggtgc acctggatta cttcggccgg
    25681 gaataccggc gcagcatcga actcttgtgc aaaaacggca cggtcacggc ggatttcggt
    25741 gcgggcacgc tgacgctgga ggacggcacg gtgcagaatt acgcagagcc tgtgaacgag
    25801 cgttatctgc gggagatggc ctattttctg gattacgccc aaagcggcgc gggacccagc
    25861 gtgaacacgc ccgagaccgc gcttgacgta ttaaaactca cattggggga actgtaaaag
    25921 tatggaacaa aagcgtgtgc tcatcaccat ttgcggccgc gcgggcagca aggggtttaa
    25981 aaataagaac ctgaagatct tcgacggcca cccgctctcg tattacagcc tttctgcggc
    26041 ggagctgttc atccggagca ggccggacct tgccgtcgac gtgtgcctga acacagacag
    26101 cgggctgctc atcgacgtta tcaccaaaaa atatcccgag gtgacggtgc tgacgcgccc
    26161 cgaggagctg tgcggcgaca tcgtgccaaa aatgcccgtg taccagtaca gcctgcggca
    26221 gatggagcag aaaaagggat acgaatacga tactctcatc gacctggata tcaccagccc
    26281 tctgcgccag acgggcgata ttgagggcgc gtatgaggtg aaggcatccc ggccggacct
    26341 ggacctgatt ttttccgtca cgcccagccg ccgcacacca tacatgaata tggcaaagct
    26401 cgcgggagac catgtggaga aggtcatcga ccaccacaat accgcgcgcc agcagacgcc
    26461 gcctgtgttc gacatcaacg cgtccattta tgtgttcgag cggcgcttcc tcattgagaa
    26521 caccacgggc tttgtctggg acggcaaatg cggcatgtac cagatgttcg acaccggcat
    26581 catcgacatc gattccgaag aggactacct gttgatggag gccatcgccc attatttgta
    26641 tgccacctac cccgcgttcg gggccattca aaataatatc agaaagtaga ctgggaaact
    26701 atggcgacac caagcgaaaa acacgagata cactgcgcca agcgctatct ggaatatacc
    26761 gaaacgccgt tttaccgcgc gttccgcgac agggaaaatc ccgagagctt tgactccaac
    26821 tatgccgtgc tcaagcgcat ggaggaagcc gagcagagcc ttgcggctgt ggaaagctac
    26881 tatgagggcc ttgacgtcac gccaaaattc tattccaagc ccgacagcgt gacgctggag
    26941 gagagccgcg ctttttttga ggcgcacggc tatgccgtcc acacctttga atgccagcgt
    27001 atgatgctgc tgacgcatac aagccccgag ctgctggtgc acaaatgtcc ggtgcagatc
    27061 tttgcaggcg cgcccctgac aggagcggcg gcgcagcttg tgacggagag ctgcggcgaa
    27121 aaggactttg gcctgcggct catcgacaag cagctgggcg cgggcgcgcg cgtttcgttt
    27181 gcatacaacc gcgctgagat ccccgtcagc ttctgcgtgg gcgaggggta cggcagtgcg
    27241 ttttatctgt cggacgtata tacggccgaa aatttccgcg gccagggcta cgcggccgcg
    27301 gcggtgctgg cgatgctggg ctttgcacgc aacccggata tggggtatac cgacgtgttc
    27361 ctctacgccg acgacccggc ggccatccgt ctgtacgaga agctgggctt ccgcggtacg
    27421 ccggtgcggg aataccgcgc gttcaagggc ggcctgcccg atctttacac cgaagcgaag
    27481 gagtaagagc atggcgctgc atgtctcgcc cgaaatactg gagcaatacc atggctttta
    27541 cagccttgcg ctggacggca cgacggatga cggctgcgcg ctggtgctgg agaatgtgcc
    27601 cgccgcgggc ctgcgcgccg cgcttgaggc cgtgccggag ccagacgcgc tgcggacggt
    27661 gctcatcaaa agctgtccgg agctgcagag cctttccttc cttgaaaatt ttcccgcgct
    27721 ggaatgggtg tatgtgcgcc gctgcccggc ggtggccgcg ctgtgggata ccgcgcgcac
    27781 gcccgcgctg cggggccttg ctgtgacgga ctgcaaaaag ctgcgggaca tcagcgggct
    27841 ggacggcgct gcggcgctgg agcacctgtt tttgcagtac agcgcctgga gcgccgtaaa
    27901 gctggagagc cttgcgcccc tgcgggggct gtgcgcgctg cgcacgctgg acctggggtg
    27961 caggggcgcg aaggacaagg tgaagctgga tttcaacatg ctgtatccgc agctggactc
    28021 cctcaccatc acgcccagcc tgaaggaata tttcatcacc aatacggagg cggggcgctg
    28081 atggagggac agaggcgcat tttggacctt tgcgtggacg cgggcgaaac gctgctggcc
    28141 aacggcgcgg aaatttaccg cgtgcagcag acgatggaga ttatcgcgcg cagctacggc
    28201 gcgcgcagct ttcatgtata tgtacttacc aatgggctgt tcgcctcgat ggaggacggc
    28261 ggtgcggtgc agagcgcggc ggtgcgcagc acgccgcgcg tggagatgaa tttgggccgc
    28321 atttcggcgg tgaacgcgct ctcgcgggag atcgtggcag ggcgcgtgcc gctggaggaa
    28381 gcctttgcgc ggcttgcaca gatccgcgcc acacgtgggc tgccggactg gctgatggtg
    28441 tcggcctcgg ccttttccgc cgcgtgcttt tgctatctgt tcggcggctc ggccgtggac
    28501 gcattcacgg cgctggcgcc gggctttgcc gtggggctgt tcacccgcgc gctggcgcgc
    28561 gtgcgcacgc cgctgaacaa gctggtctcc aacatgcttt cggcgggcgt ggtggcgctg
    28621 gtcagccttg cctgtgtgca ggcgctgggg ctggcgggcg ttgcgtgcag cctggataag
    28681 gtcatcatcg ggggcatcat cccgctggtg ccgggcgtcg cgctgacaaa cggcatccgc
    28741 gacgtggcca actgcgatta cctctcgggt accattcgcg ccatcgacgc ggtgctcatt
    28801 gcggcgggta tcgcgctggg cgtgggcctg gtgctcaagg gcgcggctct gctgccgggg
    28861 gtgacgatat gatatgggag cttctggtgc agcttgccgc agccttcggg gcgacggtgg
    28921 gttttgccgt tctggtaaac gcgccgcccc gggaatttgt ctgggcgggc gtgacgggcg
    28981 ccgtgggctg gggctgctat tggctgtacc tgcaatggca gccatcggtg gccgtggcct
    29041 cgctgctggc ctcattgatg ctggcgctgc tttcccgggt gttttcagtg gtgcggcggt
    29101 gcccggccac agtgttcctc atcagcggca tcttcgcgct ggtgcccggc gcgggcattt
    29161 actataccgc ctattatttc atcatgggcg acaacgccat ggccgtggcc aaaggtgtgg
    29221 agacgttcaa gatcgccgtg gccctggccg tgggcatcgt gctggtgctg gctttgccgg
    29281 ggc
//
LOCUS       DAWBSB010000002        29063 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_439_length_29063_cov_3.082219, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000002 DAWBSB010000000
VERSION     DAWBSB010000002.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 29063)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 29063)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..29063
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_439_length_29063_cov_3.082219"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2871..2880
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6213..6222
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7904..7913
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10212..10221
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    17094..17103
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    19024..19033
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    21727..21736
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    28820..28829
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4935 a         9731 c         8274 g         6043 t
ORIGIN      
        1 gtttacaacg cgccccgaca tatggccgta aatggtagca aggccggagc cgtgggatat
       61 ctttacggaa attccgtagg tccagtggtt gttggtgctc accacgccgt ccgcagccgc
      121 atagattttt gtacccgccg gggccggcac atccatgccg cggtgcacgt cccacttgcc
      181 gtagatccag cgtcccacgc cgaaatcgga ggaaagtttc gtataccccg ggataggcca
      241 ggcaaagccg ccctgattgt actcgaaatc cacgttgttt gccgccgtcc actgccgcca
      301 ttcttcctgc gcctgctcgt actgcctctg ggtttccgcc agagcctctt catttgcggc
      361 aaggtccgcc tcagccgcgc tgatggctgc atccgcctgc tggatggcat tggaatacgc
      421 cacggcggtg tctgtcagct cctgctcgcg ggcggcaagg tccgcaagct gctgctccag
      481 ctcggcgcgc tgggcctcca gggccgcctg ctcgtcccgc agctgctgta cgagctgggt
      541 atcatgctcc gatatctgct gcaggttttc ggcaaacagc agcatctggg aaacgtcgtc
      601 gatgcccaga agaattgcaa ggttcgactg ccggctcatc tcgtacatgc ccttgaggcg
      661 ggattcgaac atgctcttat tatacgccac attggccgct ttttccgcca cctcggcatt
      721 tttcagctca atgctctgct gttgggccgc gatgtcctcc ttgatggcgg caagctgcgc
      781 cttcaggtta ttggcctgtg cctgataata ctgcttggtc tgctcggatt tttccttgct
      841 gttctccacg gcgctgatgt tggatttgat gtcctccagc tccttgcgca gacgctccag
      901 cttctccgcc ggcgtttcgg cataggcaaa gatcagcggc acgccgatgg tcatcaccat
      961 cagtgcggca agcaccagac atacgacgcg gaccagtttt ttatgctttt ccatagaaaa
     1021 ctcctgtgtt caaaaaagat cacaccttca ggtgcttgcg gatgctggac gcgcagccca
     1081 cgccgccgat ggcgacgccg aacagcacaa acccgcccag cagcggccag atgatatcca
     1141 tgaacggcca catgcatttg gtgaactcgt tcagccaaag cgcctccggc tgcagcagcg
     1201 cctgctccag cccgtaatac gcaccgccca caacgaccgt ggccagcacg cccgagatag
     1261 cgcccaccgt catgccctcc acaaagaacg gcagccggat gaacgcattc gtcgcgccca
     1321 cgaatttcat gatattgatc tccttgcggc gggcaaatac cgtcaggcgg atggtgttgc
     1381 tgatgaccac cacgctcacc acgcccagca cggccacaag gccccagccg atgtagttga
     1441 cgatgcgctt caggttcacc aatgtactgg aaagctcgga gggcgaattc actttgtaaa
     1501 acacgttcgc atcgctggcg gtgccgtcct cggccgtggc cgtatacgcg cccatggcct
     1561 tgatgcgctc cacggtgggc gcaaggtccg caacgtctgt caccgacacg cggaacgaag
     1621 cgggcagcgg attttcggtg ttcccttctc cggcataatc gttcatgata tcgccgtagt
     1681 cgcccagata ctcccgcatt tcctccacgg ccgcagcctt ggggacgtat tcgcaggcag
     1741 ccacgttccc gtcggcgggg atctgcgttt tcagcgcctc cacggtctcg gcgggggcgg
     1801 cgtcatcgat gaaaaagacg atctcgttga gcgtttcaag ataatccaca tagcttgtca
     1861 cgttgacgga cagcagcgcc gccacgcccg tgatcatcag gcatgccacc agcacgccga
     1921 tggatgccag cgtcatcagc cggttggcca ccatgctgtg ccatccctgc ttgacaagat
     1981 acttaaagct ggaccatttc atacctgcgc ggcctccctc ccgtaccggc cgaggccgtc
     2041 gtcgaccacc tcgccgtggt cgatcatgat ggtgcgcttg cggaaccgcg cggccagctc
     2101 gcgttcatgg gtgacgacca cgaccgtggt gcccacgctg ttgatggcct gcagcagctc
     2161 catgatctcc acagacagct ccgggtcgat attgccggtg ggctcgtccg caatgatgat
     2221 gggcggggaa tgcaccaaag cccgcgccag cgcaatgcgc tgctgctcgc cgccggaaag
     2281 ctcctgcggc aggctgtgcg ccttgcccgc gagccccacc aggttcagca catagggcac
     2341 gcgccgcttg atctgccgct cggggatatt cgtcacacgc atggcaaagg ccacgttctc
     2401 gtaggccgtc atcgtgggaa taaggcggaa atcctggaac accacgccca gcccgcggcg
     2461 caggtaggga atttcccgct gacggatgct ggaaaggtcg cgcccgttga cgaaaacctt
     2521 gccctcggtg gctgcctcct cgcgcaggat cagcttgagg aacgtgcttt tgcccgcgcc
     2581 ggaatggccc agaacgaaca cgaattcgcc ttttttgatg gagagattca cgttgtggag
     2641 cgccacgata tccttgcttt tggcgtccag cccggtgtat atcttcttca cgttttcaaa
     2701 acggatcata tcggtagttg ccatatcatt ctcctatggg ggtcacggtt gagtgcgtgc
     2761 gcgcaaagca ccgccctgcg cagggaatac gcgcctgccg cccgcgatat tgcaccgcgg
     2821 gcacggcata tgccaaaaag gcgccgctgc ggcgaaagca ggctttgccg nnnnnnnnnn
     2881 gctctttttt cttaatactt cgcggggttg gcgctcaggt atttcttgac catcagcgcc
     2941 accttgaaca caatggcgtc gtcgaattcc cgcaggtcca ggcccgtcag ctttttgatt
     3001 ttttccagac ggtacaccag tgtgttgcgg tgcacgaaca ggccgcggct cgtctccgaa
     3061 acgttgaggt tgttttcaaa gaagcgctgg atggtgaaca gcgtctcggc gtccaggctc
     3121 tcgatgctgc cctgtttaaa cacttctttc aggaacatct cgcacagcgt ggtgggcagc
     3181 tgatagatca ggcgggcgat gcccagatgg tcgtagctga cgatcgcctg ctcggtgtcg
     3241 aacaccttgc ccacctccat ggcgatctgt gcctccttga agctctgggc caggtccttc
     3301 acattggcaa tgggcgtgcc gatgcccaca acggccttga tgtaatgctc gccgttgagg
     3361 gtgtccacaa tgctggcggc cagcttttcg atgtcgtgct ggtcgatgcc gcgcttgatc
     3421 tccttgacga ggacggtgtc gttctcgctg atgttgaaca cgaagtcctt ctgcttatcg
     3481 gggaacaggc cgcttacgac gtcgtaggcg gaaatgtccg cgcccgaggt aacgcggatg
     3541 agcagcacca cgcgggacac gtccgttgcg aaatgcagct cgcgcgcctt ggcatagata
     3601 tcgccgggca ggatattgtc cagcaccaca ttcttgataa agttcgcctt gtcgaacttt
     3661 tcgtcgtggt actgcttgat gttctgcagg ctgatggcca gcagcgccgc atagcgcagg
     3721 gcgggctcgt cggttccctc caccagaacg gcataatcgt tgcgcttgtt cgaactgaac
     3781 agatgatagg tgtagccgtc ccgcacgaac gcgtcgccgg ctgtcagccg gtcggacgcg
     3841 atgccgtccc gcacctcgcc gatgcggttc aagtcagaac aggagatgat ggtgcccgtt
     3901 tcatcgacaa cgcccaccat gcggtcgatc gcatctttca tctgatagac aacgccctga
     3961 aaaactctgt tcgccataat acccttttcc cttctgtgct gcggcgctct gcatgcgctc
     4021 agttgtgaat tttaacaata aggtgtcgct ttagtctcta ttttacacaa tagttctgac
     4081 ttttgcaaca tatttcaaca aaataaacgt cattttattg tgtaatttgg gaaacagggc
     4141 gcaaacactg catttatcgt acatttttca tgtgaaaaat gttttaaaaa cctgtgaaca
     4201 gcgcgccgaa aatgcagttt ccagcatttg gcccggaatt ttttcctgtt ttctagccgc
     4261 cgaggatacg cgccacctct gcgagcgaaa gctcgcggaa gccgcccggc cccagcgcgg
     4321 cgtccagcgc caggccgccg atggccgtgc gccgcagctc gttcacgccc gcgccgtata
     4381 cgccgaacat gcgcttgatc tgatgataca cgccctggcg cagcaccaca cgcgcgctgc
     4441 acgggccgtc cggccccggc gaaagctccg cgggctgcag ccgctgcccg tccgccagcc
     4501 ggacgcccgc cgcaaaacca gccgccatct ccggcgtgag gggcgtgtcc agcagcacct
     4561 cgtaggtttt tggcacatgc cgcctgggcg cgaggatatc atgggcgaat gcgccgtcat
     4621 ccgtcaccag cacaaagccc gtgctggtct tgtccagccg cccggcgggg aacaggctgc
     4681 ggcgggggta ggccctgccc acaaggtcca ccacagtcac gtcgtttttg tccgcggacg
     4741 cactcaccac acccttgggt ttgtccagca tgatgtacac atactgctgc aggacgagcg
     4801 gctgcccgtt cagcgtgaca gccgcgccgg ccggcacctg agcgtccgcg ctgcggcaca
     4861 ccgcgccgtt cacgcacacc agccctttct gaatggcccg ccgtacgtcc gagcggctct
     4921 gctgggccgc gcccgccaca tacttatcca gacgcatgcc gcgtcccctc cccccgcgcc
     4981 gggcccgggc ccggcttttc cttatcaatt attatacggt ccagtatagc acaaaaaagg
     5041 ccgaaacggg tagtgtttcc cgtttcggcc ctgggggagc ggaggccgcc gctccgctca
     5101 ttggcggtaa tagtgatagc tgcggccgtc gctttcaaat gtgatcacta tttcgttgta
     5161 agaaatatag tcccatgcgg caaaccgtgt gccactggaa gcatagtaca tgccatcgct
     5221 ctcaaaattg tatttaccgc tcggaacata acgataccag aaatcaaact cgttgttttc
     5281 cattgcatat tcaaaaacat cgccatccac cgcgacccat gttccatcca aaaagtagtc
     5341 tataagatat ctgtccatac atataggttc cacatcaata tactggtcgc tccattcgag
     5401 catctccaat gcattttgga cttcccgtgt cattttataa tagttttcag ataaaacagt
     5461 gtgcgcctta cacgcttcca cataaacaaa agcgtcttcg tagttttcat aatccggtat
     5521 gatctccagc cactccattg ccgcactgaa acgattttcc ccgtagtgct ccaatgccgt
     5581 gtcgtaaatt tccggtatct gcgcctgcgc ctgccggctg ctgtctaaaa agccgcccag
     5641 attgttgtac aatttgtatt tcgtaaaagg cgagcccacc gtctgcgcac ggatatatgt
     5701 gttgcacttt tccgccagct ccccgctgtc cgcgtaatcc ccaagctccc ggaacgcctg
     5761 cgccgcctcg ctcagctccc cgcgctccac cagcacccag gccgccgtat attgcccccg
     5821 ctgctcgtcc agttcccaca gccacggcgc gcgctcgtac cattcgtaag cctgtgtata
     5881 ctgttcgtaa gcctgcgcgg tttcaatttc gttccacgca tacagcgaat accccaaaaa
     5941 gccaccgccc agcagcacca gcggcagccc cacggccagc gcaattttcc agccgcgctt
     6001 tttccggcgc ggggcctgcg gtgtgcaaac ggcagagggc gcatatcccg cacccggcgt
     6061 accgccgctc ccctgcgggg caaacggccc aagcggcgca gcttcctgca gggccgcgcg
     6121 ctgcgcctgc agcagctcct gtgcaggcac tgtctgcgcc ggctgtgcgg cggcaagctg
     6181 tgcttccacc tgcgccagct cctgtgcaag ctnnnnnnnn nnagctgcgc cggggcggca
     6241 atgcggtaag cctcgttcag cgccatggcc cggttcagca gcgggacaag cggcgtttgc
     6301 tcctcaaaca ccagctccgc gccgcccgca cgcgcgatca cgtcctgcgg ggcgcggccc
     6361 tctgtaagcg catacagcac ggcccccaga aaatagactg cagcggccat acccgcgctt
     6421 ccgttgtaac attccggtgc ggaatacccg gcgcacacgg aggcgccggg ccttttgccc
     6481 gcgccgtgcg cacgcttcag ctccagcgta ccgctccgca caaggatatt ttccggatgc
     6541 agcgcccgcg agacctccgg catcgaagcc agccgttgcg ccagctcccg caccgcccgc
     6601 gttgcccgga gcctgtccat ccgtccgccg ccggccaaaa acacaggcag cgggacagcc
     6661 ttttcccgca tgggcttccc ggacgccgct ccccggcggc gcagccgcaa aagcagcgcc
     6721 gcacacacgc ccagcgccgc cgccacagcc agtacaacca gcagcacgcc cgcaaaaccg
     6781 ggcgccgcct tgcggtacgg gatatcgttt tgctgcagaa cagtgagcag ctcccgcaca
     6841 tgaatgctgc cgttcacatt ctggccctcc aacattataa acgtattgat cccgacaagc
     6901 ctgccgcccg catccacaag cggcccgccg gaattgccgg ggctgatggg cgtgttcgtc
     6961 tggatcgccc acgtgctttg atagccctcc gccctgcttt gccgcaccgc gctcacgatg
     7021 ccgtccgtca ccgtgatgct gtccacgccg gtggcaaatt cccggctgaa atcatccgcc
     7081 gcgcccggat atcccagcgc gtataccgcc agccctgttt caaccgcgtc cgagcggagc
     7141 ggcagcccgg gcatgccgcg caggggcttg tctgttttta agatacagat gtcctttccg
     7201 ggcagatcta cgtagacccg ggccgtcgtc caccttgtgt cgtccactat gacctcaatc
     7261 gtttccgcgc cttcgatcac atggtggttc gtcaccacat agacggaatt tctggacacc
     7321 gcaaagccgc tgcccatcgc cctttccgtc ataatgcgca ccacgctctc acgcgcgttc
     7381 agcaccgcct tcggcacatc cgacgcggca agcgcgcaca cgcccagcgc cgccgccagc
     7441 agcattgtgc ccaaaacggc ccacagccgc ctgcgtcccc gttttttcaa catcacagtt
     7501 cccccgcttc ttctgctctg gtttcattgc accatatttc ttagctcgca cccgctgacc
     7561 ggacatagca taccatgacg ccgccgcagg cggcttcaac aaaactttgg ccgccaggat
     7621 tttgtacgcc ttgaggttct cacgctcatg cgcggcagag cgtcggtgca tgaagcatgc
     7681 acggccgcac aagcggctgt ccaaatcaat tatatcagaa gcagccgctg aaaagaagcc
     7741 cgtcaaacaa agcggcatgc cgaaaaaggg catgccgctt tgtacagaat caactgcgtt
     7801 ttgcgcttat tccgtgggaa acgcccctgg ctcgatctgc gcgtcggcgt ccgcctgggc
     7861 ctgcgcttcg gcggccgcgt cctccgtcgg cccggccgta tccnnnnnnn nnncgtctgc
     7921 agccgcgttt gcaccgctca cgcccgcgcc cgcggcggct gcggaatcat ccgccggaaa
     7981 ggcggcgcca aagcccgcgt ttgccgccac ttcttcgtct gtcagcgcaa ggcttgcccc
     8041 cgtgggcgcc gccgctttca gcggaagcac ctcgccggag ggcttttgca ggatatacgc
     8101 gcccctggcc tcgtttttgt acacgagcac cacgccgtag gatttctgtg tttcggtgct
     8161 ctgcgcagga atgagcacca cccatttgtc taccttttta tttttgacgc gggagaggtc
     8221 cagcccgctt tccttgatca cttcattgaa cgccttgaag ttatcgtccc acttgcgcgg
     8281 cacggtgacg gtgctcacct ccgcggtagt ggggtccact tcgacgccat acccctgcag
     8341 gaacgtcgcc acgtcctgcg cgccggtgat ctgctgcgaa agcgcgccgc cctgcgcggc
     8401 cgcatccact gcgtcgccct tgttgcgcac cacgcccacg gtaatggccg cgcctgccag
     8461 caccacgccg cagacggcca ccacagccag tttccgcagc ccgcccttgc gtaaagtaca
     8521 tacaaacatt gcacgtttcc ctccttacga ttttcggggc ggtgtgccgc cccaacccct
     8581 tccgcgcggc acgcgttccg ggccggctga actggtttct actgaaattc tatgaggggg
     8641 ggcgtatgtt tagcacagac gtttgcggcg gacatgccgt gaatttttct attgacaaaa
     8701 cccgcgcgga cggggcatca tatattcata acaacaaacc ggaagaaagg gctgcacgta
     8761 tggtactgac aatagacatc ggcaacacca acatcgtttt gggcggatac gaaaacgggg
     8821 cgctgggatt ctgtacccgc atctccacgg acaaaagcct ggaggcggac cagtacgcgc
     8881 tggagctgtc cggcatcctg aacctttaca aggtggcggg ggaaaacatc gaaggcgcca
     8941 tcatttccag cgtggtgccg cagatcaccg acacagtggc gcgggcgctg cgcatgtttg
     9001 cgggcgtgga accgctgaag ctcacgcagg agctgcccac aggcgtaggg gtgaacatcg
     9061 acacaccctc ggagctgggg gctgacctgc tggccggagc catcgccgca aaagcgctgt
     9121 atccgctgcc ggctatcgtc atcgacatgg gcacggccac aaagatcacg gccgtggacg
     9181 agcagggcac ggtgcagggc gtgagcatca tgccgggtgt tttcatcagc ctggacgcgc
     9241 tggtgaacgg cacgagcctg ctgaagggca tcgccaccaa cgcgcccgtg cgggccatcg
     9301 gcaaaaacac cgtggaaagc atgcagagcg gcgtggtatt tggtgcggca agtatgctgg
     9361 acggcatggt cgagcgcttc gaagccgagc tcggccccgc aaaaacgctg gtggctaccg
     9421 gcggtgcggc gggcattgtt gtgccgcact gccggcacag cgtgcaatat gtgcccacac
     9481 tcattctgga cggcctttat gccgtgtact gccgcacaca cgcggccctg taaaaaacgg
     9541 gcgtaaaaag cacgggcgcg ccttctccct tttgcgcctc cccctgcatg cccacaaaac
     9601 agaaaagccc ctctgcgcgg cgcgcagagg ggcttttccg gcgttttgta ccggggatgc
     9661 gtttattctt cgtccttgcc gccgcagcag ccttcacagc cttcgcaggc gtcgtcgtcc
     9721 tcgctgaagt cgacctcaaa cttcgtgccg cagttggggc aggccagatc gtcattggcc
     9781 agcatatcct catccacgca cacaacctca ccgcactgcg ggcaggtgat ctcatacagg
     9841 cccgcctcgt actcctcgtc gtagtcgtcc tcgtcttcgt ccccgccgtc ctcgtcgaac
     9901 aggaagtttt ccacgtcctc caggtcctcg ctcatggcgt cgacctcatc gtacagcgta
     9961 tccacatcct cgtcgatgtt ggtgacctcc ttggccatct cctccagcac atccacaaca
    10021 gcggcaagca gcttgccctc cttggtggca gtgtcaaatt ccatgccctc catcatccct
    10081 ttgatgtagg cgactttttc tttcagttcc atgggtcata ccctcctatg cttttattct
    10141 ttcattgcaa tgcaatgaaa agcgtcttca ggcggcatcc gccgcgttcc ggcccggagg
    10201 ccgtttttta gnnnnnnnnn nccatatact cgccggtgcg ggtgtcgatg cggatcatat
    10261 cgccctcgtt gatgaacagg ggcacacgga tctccgcgcc ggtctccagc gtggcgggct
    10321 tgagcgtgtt ggtggccgtg ttgcccttga agcccgggtc cgtcttggtg acctccagga
    10381 ccacaaagtt cggagcctcg acgccgaaca ccttgccctt atagctcagc accttgcaga
    10441 cctcgttctc cttcacgaat ttgaaggcgt cgcccagctc gctctcatta agggggatct
    10501 gctcgaacgt ttcattgtcc atgaagtagt acaggtcgcc gtcctgatag gaatacgtca
    10561 tttcgcggcg ctcgatatac gcggtgggga acttggcggt ggggttgtag gtgcgctcca
    10621 ccacagagcc cgagatgacg ttgcggatct tggtgcgcac gaaggccgcg cccttgcccg
    10681 gctttacatg ctgaaattca acgatctgga agacgttgcc gtcctcttca aacgtgatgc
    10741 cattgcggaa atcgccagca gaaaccatag aatgaatcct ccaatattat cccggccgcc
    10801 ccggcctttg gccgccgggc ataccagttc attttatcta ttctgatttt acacaatatg
    10861 cgcctgtttt gcaagggttt tcggccattt taagccattt tcccccaaga cggccgcgct
    10921 ttccccgccg ggcgggcagc tgcccgccca cgcgtcacag cacgatcagc tccttcgggc
    10981 tttttcccac gatctcgcag ccgtccggcc gcacgaaggt ggtgtcctca atgcgcacgc
    11041 cgaattttcc cggcaggtag atgcccggct cgatggtcat gaccatgccg gactgtatga
    11101 tgtcgctgca ggtggggctg taacgggggt tttcatggat ctcgatgccc acggcgtggc
    11161 ccagcccgtg gccgaagcag ccctcgtagc ccgcgccgta gatgatatcc cgggcaacct
    11221 tgtccacctc gcagcctttg tagcccgctt tggcgaatcc gcagcaggcg agctgcgcct
    11281 tcaaaacggt atcatacacc tttttctgct cgtcggacac atggcccacc gccaccgtgc
    11341 gcgtcatatc ggtgcagtat ccgtccacgt tcgcgccgaa gtccatggtg acgaagtccc
    11401 ccgcctcgat gcgtttgtcg ccgggcacgc cgtgcggcag gctcgttttt gcccccgaga
    11461 caaagatcgt gtcgaacgcc agcttgcccg cgccgtggct cttcataaac acttccatct
    11521 ccagcgcggc gtcgatttcc cgcacgccgg gtttgatgta gcccagcatg tgctcgaagc
    11581 aggcgtcggt gatgacctgt gcgcggcgga tggcgtcgaa ttcttcctcg gacttgacgg
    11641 cccggatggc atcgatggcg tcggagagca cggcgccggt gtccacctcc acgcccggca
    11701 gcgcggcgcg cacacggtcc gcataagcca gtgtggcctt gttttccaga tacagatgct
    11761 taacgccgtg cgcggccagg atgtcccgca cctgctccag tgcctttccc tctaagatga
    11821 cctcggcttc cttcacagtc gcttcggcga tctcgatgta gcgggaatcg atgatgaagt
    11881 aggttttatc cggcaaaatg agcacggtgc ccgcgtcccc ggagtcaaag cccagaaaat
    11941 aaaagcggtt cacatccgtg gagacaagcg ccgcttcaaa gcccccgggc atggcggcaa
    12001 gcagtttatc ggtacgttgt ttcatatcgg tccaaccttc ttattccagt attggcggcc
    12061 gggacgcttc tatgcgtccg gtggtttgcg gtcttcctct tttccgtgga cacgcccgcc
    12121 ttttttataa taccgcgcca gcacgggttt ttcaagcgcg cgtttcaggc gtatggtcgc
    12181 tttggtgtcc tgccgcatgg gctgcaccag cagcaccggg atgccgtaca ggttcgcacc
    12241 cagcgcgtcc gtaaaaatct ggtcgcccac cagcgccacc tcgcgacggg atacgcccat
    12301 ggcccggcgc gcccggcgca ggccaaacgg cgacggctta caacagaacg cctgatactc
    12361 cagccccacg cgctttgcaa agggagccac gcggccgggc atattgttgg aggcgatggt
    12421 gagcttcacg cctgcggcgc gcatggtatc cagccacgcg gccacgtccg gcggcagctc
    12481 ctggctgccg tgggccgtga gcgtgttgtc cacgtccaga atgagcgcgc ggagcccccg
    12541 cgcgcccaaa aactccggcg tgatgcaggt gatgcgctca aaaacgatcg tcggtgtaaa
    12601 caggggcatt ttcggttcct cactttatca gttgtgctcc gtgcggacgt gcatgtccgg
    12661 cgggcacggc agccgccggg ccgccttttc ccccgccctc ttttggtgca aaaaaagcgg
    12721 gccgtaaagg cccgcctgtt cgcttacttg ccgcgtgtcc gctattctta tttgaacttg
    12781 cgcttgcggg ctgcttcgga cttcttcttg cgcctgacag aaggcttttc gtaagcctcg
    12841 cgtttacgca cttcagcaag cacaccggag cgtgcgcagg aacgtttgaa acgacgcagc
    12901 gcgctgtcca gcgattcatt gtctttcact ctgatttccg acatctttgt ccctccctta
    12961 aacctgaggt aggcgccggc ccccctctat gcgggaaact gactatattt gattatacaa
    13021 tgcagacggc tcgtctgtca acagataatt gtgaacatct ttcctacctt acagcaatat
    13081 ttacaagttt gcccggcacg tagatctcct tgcagatggt tttgccctcc agcgctttcg
    13141 caacattggg ctccgccttt gcggcggcaa tggcgtcctc tttggccgcg cccgtggcca
    13201 ccgtcagctt ggcgcgcagc ttggcgttca cctgcacggc gatctccacc gtgtcctcca
    13261 cgcatttcgc ctcgtcgaat tccggccact tcgcgtgggc aagctgcccc tcgtagccgc
    13321 actgctgcca aagctcctcg gtgatgtgcg gggcaaacgg gttcagcagg atcagcagcg
    13381 ttttgaactc gccgcgggtg atggattttt tgtcatagat ctcgttgagc aggctcatca
    13441 tggcggcgat ggcggtgttc attttgagca cctcgatatc ctccgtcacc ttcttgatgg
    13501 tgcggtgcat cgcgccttcc aattccgccg aatagccgtc gccgtccaca agaatatccg
    13561 ccaggctcca cacacgctcc aaaaagcggc ggcagccctt gatggacgcg gtgttccacg
    13621 gcgcgctctt ttcaaaatcg ccgatgaaca tctcatacag gcgcatggtg tccgcgccgt
    13681 agtcccgcac gatatcgtcc gggttcacca cgttgccgcg gctcttggac atcttctcgc
    13741 cgttttcgcc caggatcatg ccgtggctgg tgcgcttctg gtacggctcc ctggtgggca
    13801 ccacgccgat gtcgtacagg aacttgtgcc agaaccggct gtacagcaga tgcagcgtgg
    13861 tgtgctccat gccgccgttg taccagtcca ccgggctcca gtattccagc gcctcccggc
    13921 tggccagcgc gtcgttgttg tgcgggtcca tatagcgcag gaagtaccag gacgagcccg
    13981 cccactgggg catggtgtcc gtctcccgtt tggcggggcc gccgcagcac gggcaggtgg
    14041 tgttcaccca ttcggtatgg cgtgccagcg ggctttcgcc gtcgccggtg ggctcaaaat
    14101 ccttgatctc cggcagcacc agcggcagct cgctctcagg cagaggcacc cagccgcatt
    14161 tttcgcaatt caccataggg atcggctcgc cccaatagcg ctggcggctg aacacccaat
    14221 cccgcagctt gaagtttgtc ttgggcgtgc ccaggccctt ttcggccagc cattcggtga
    14281 tggccttttt cgcctcgtcc acgctcatgc cgtccaaaaa gccgctgttc accatcacgc
    14341 ccgtggcaca atcggtgaaa gcctccttcg tcacatcgcc gcccttgacg acctcgacga
    14401 tgggcagatc gaattttttc gcgaaatcat agtcgcgctc gtcgtgggcg ggcacggcca
    14461 tgatggcgcc cgtgccgtag ctgatgagca catagtcgga gatgaagatg gggatctctt
    14521 tgccgttcac ggggttcacg gcgcgcacgc cctccagcct tacgcctgtt ttgtcctttg
    14581 caagctcggt gcgctcgaag tcgctcttct tcgcagcggc ctcctggtat tcccgcacgg
    14641 cgtccaggtt ggtgatcttg tccgcccatt tttcaatgca gggatgctcg ggcgagatga
    14701 ccatataagt cgcgccaaac agcgtgtccg gccgggtggt gtagatgagc agctcgtcgc
    14761 ccgccgtggt ggagaacttt acctccgcgc cgtggctgcg gccgatccag ttcttctgct
    14821 gcgtcaccac gcggtcgatg aagtccaggt cctccagatc atcgatgagc cggtccgcgt
    14881 aagcggtgat cttcagcatc cactggcttt tgacgcggtg caccacctcg ccgccgcagc
    14941 gttcgcacac gccgcccaca acttcctcgt tggccagcac caccttgcag gaggtgcacc
    15001 agttgacgct catctctttt ttgtaggcaa ggcccttttt atacagctgc agaaagatcc
    15061 actgggtcca tttgtaatag ctggggtccg tggtgttgat ctcccgcgtc cagtcgaagg
    15121 aaaggcccag gctcttgagc tggcttttga agcgcgccac gttgtttgcc gtcaccacct
    15181 cggggtggat gtggtttttc atggcgtagt tttccgtggg caggccgaag gcgtcccagc
    15241 ccatgggata cagcacgtta tagccgcaca ggcgcttctt gcgcgccacg atgtccagcg
    15301 cggtatagct gcgcggatgg cccacgtgca ggccctggcc ggaggggtac gggaactcca
    15361 ccagcgcata gtacttgggc agcgaaaaat cctgcttggc cgcaaaggta ttctccgcat
    15421 cccaaatgtc ctgccacttt ttctcgatcg ctttgtaatc gtacttcatt ttatatgtca
    15481 catccaaaaa agatttgcag attccgggcg gcgcgccgcc ttattcctcg atgaaatcca
    15541 tgggcacgtc ttcgccgtcg gcccacaaat ggtccagatc gtaatagtcc cgcgcgtcgg
    15601 ggctgaacac atggacgatg acgttggcat aatccaggat aaaccagttc tgggtgttga
    15661 agccctctgt acggatgggc gcgacaccct tttccttgaa ctggaactcc acctcctcgg
    15721 ccaggctttt cacatgggtg gaggacgtgc cgctggcgat gacgaaataa tccgccagca
    15781 ccgtcaggtc gcgcaccttc agcaccttga cgcgggtagc cttcttgctg tcgagcagct
    15841 tggcgatctg catcgccagt tcttttgatt ccatgaacac tctcctgttt caaatagggt
    15901 aaaaacgttc tttcttccac tattatgccg cgggctgctg cacggatgca gcgtttgcct
    15961 gctgggcggc ctgctgggcg tctgcgggcg cgtttgcatc gctgccgccc acggcgtcca
    16021 cctcgcccat gcggcgcacc tccgcgccga taacctcgct gcgtgtgccc cagtccggcg
    16081 tgccgatctg ctgtgccgcc agcgggtcgt ccgccgggcg gaaatattcg ttcagaaatt
    16141 cagcggtctc cgccttggcg cacaccatca cgtcctggcc gttgtacagc tcgccgcggg
    16201 ccacgggcag gcggccgatg atgatatttt cgctggggat gctcagcacg ctcatgccca
    16261 gctgaacgag ctccataaaa ttgaggctgg tgttgatgta tttctgcacc acgggcgtca
    16321 gcttgatgat atcgcccacc gtggccgtcc gaacgcggcg gaacagcgcc gagtagaaat
    16381 actgctggtt ggccaggcgg tcgatgtcgc cgcgcggcgt ggccgaggtg tccttgcgct
    16441 ggcgcaggaa aaattcgcac tccgcgccca tcaaaacgcg ccagccctgt tccagccggc
    16501 tgccgtcata ttccatagtc acgggcacat acacctcgat gccgccgaac acgtccacga
    16561 tctcccgcag ggagtccata tcaatggtga cataatcatc aatgggcagg ccgaacaccc
    16621 ttgtgatgta ctgggccaat gtattgacgc ggttttcctt atcggccgaa ttggcgaaaa
    16681 tgccgttgat cttgcccgcc gagccgccga tctcgcccac cagcgtgtcg cgggggatct
    16741 gcagcatggt catcgtatgg tccgaaccat tcacccgtac atacaggatc atatccgtct
    16801 ggccgatggg gtcgcgcttg acggcgtcct cggtgctgta atcgatgccc agcacaagga
    16861 tgttcatggt gccggacagc tcgtcgggca ccaatgcgtc cgcaccgctc tccggcggcg
    16921 ccaggctgcc catctcaccg gtgttgaaga tactgcccac ataccagtat atcccgccga
    16981 aagcaaggcc cagcaccagc acgatgcaca aaagcacctt ggcggcggtt ttgcccgtgt
    17041 ttttctttct gcgcctgcgc gcggaggttc catatcccgg cgcggaggaa tgcnnnnnnn
    17101 nnnccccgcc gcaccgtttc tgcccccctg ggcgggcctg cgcgccgcac cctgccggga
    17161 ggcggcggcc ccgcgcgcca cggaggtacc ggatgagcgc ccgtgcaccg gcgcgcgctg
    17221 tgctccgccg cgtacggccg cgccccccgc ctgcctgggc tgcgccgcgc gccgccctgc
    17281 cgggcgcgcg cgggaaatat caatgtcacg gggtcctcgc tgtctgttca aatcgtgtac
    17341 ctccggtgtc tttcactttc cggggcgtcc cgaaagccgc ccttcccttg caggcgcacg
    17401 gctccgccgc agcccgcgcc cggagcacgg ccgtaaaaac cgctcatttc cggggctcct
    17461 ctgcacccac ggcttccacg tcgcccatgg tgctcatctc ggcgcctgcc gcaccgcctg
    17521 tggtgggcca ttccggcgtg ccgatgtccg cggcggcaat ggggtcgtcc gccgggcgga
    17581 aatattcatt caaaagcgcg gcggtctcct ctttggccgt cacgactggc aaagtaccct
    17641 ctggtatacc gtaatatttc tccgccgcct ggtagaccgg gagcctgccg atggaaatat
    17701 ccgcgctgtc catgccgctc acctcaatgg caatgctcat cagctccagc gtggaaatat
    17761 ccgttttgat atacttctgc gccacgggca gcagcttaac gatgtcgccc acagacgccg
    17821 ttttgaggcg gcggaacaag cctgcataga actgccgctg catcacgaga cggtcaaggt
    17881 ccgagcgggg caggccctgc cggtagcgca ggaaaaattc gcagtcgccg cccagcagcg
    17941 tgcgccagcc ctgcagaagc tcgccctcca aagcgcccgt cttttcgttg taataccgca
    18001 tattctgcgg cacatagacc tccacgccgc caaaagcgtc caccatttcc gtcaatgcat
    18061 ccatatcgat ggtgacataa tcgtcgatgg gcagcccgaa attcgtttcg atgtacgttt
    18121 tcaccgcacc caggccgttg ccgtccccaa tgtccacatt tttggccagg atgccgttga
    18181 tcttgcctgc ggcgccgccc gcctccccca caaacacatc ccgggggatc tgcagcatgg
    18241 tcatggaatg ggcgtttccg tccaggcgga catataagat catatctgta ttgggatgct
    18301 tttcgtcacg ggcaacaggg ccctccgcct cgtaatccag ccccagcacc agaaggttgc
    18361 gcacgcccgc gaaatccggc gggacgatgc tgggttccgc actggtgctg acgggggacg
    18421 aaacatcgtc aaagctgccc aggtcactgc ggtcgatcag gctgtccaca tacaggtacg
    18481 cgccgcctgc cagggctgca agtaccagca gtacggcgag cgccgcgata ccggctctgc
    18541 gccggcctgt ccgtttgccg cgcgctccgg caggccgggt tcctgcttgt ccccccgcac
    18601 cggcatgcgc tttcgcgccg ccgggccgcg cagacgatgc gcgcgctgtg ccgtgctgct
    18661 tttgcgcgtc ctgctgtccc gcgtgttctc ccgccccatg aggggccggc tgcgcgccct
    18721 gcggcgccgg ggccgctccc tttttcgggc gggcgcgggt gatatcgatc tcacggttct
    18781 cctgcggtct gctcactggt cctacctccg taataccgcg cgcgcaaatc cgcgtaggca
    18841 aggagggatt cctcgtccac gggcttgccc ccggccttga gccatgcaat ggaaaggccg
    18901 agcccctcga tggcggtgcg gtccaggtcc gccagcgcgt ggcgccgcag ctgctccgcc
    18961 tcgggatagg tgcgctcgta gcttgccatg tcggcaaggt acatgatttt atccagcttc
    19021 gtcnnnnnnn nnntcatgcc gggccttccg gtggtatggc agcggatggc ggagaggatc
    19081 tccccgtcgt ccacgccgta ctgcgtctgt gcaaggatcg ctgcggccgc gccgtgccac
    19141 accggggcgg ggcgctgggc tgcattacca gctattatag cattttctgt aaatatctgc
    19201 aataatctgt cccgcggcag ctcctttgcg atatcgtgca gcagcgcggc cagcgctgct
    19261 ttcttttcgt ccgcgccgtg ggccttcgcc agcttcacgg ccagcttgcg cacattgcag
    19321 gtgtgctcat accgctttgg ggaaagattt ttctttgcaa ggcgtttcgc ctcgtccacg
    19381 gaaatcaaaa tcaggtcgcc tcgattcgcg cccgcagcgg cccggccgaa gcgtgcgctt
    19441 caaatgtcct gcagccgggc gcgtcaggcc tccccggccg cgggcgtata aagatggtgc
    19501 cgcgcaatgt acgccgccac gctttcgggc acaaggccgt ccaacggcct gccctttgcc
    19561 gccagcgcgc gcacctcggt ggaggacacg ggaaacacgg gcgctttggc aaacagcacc
    19621 gtgccgccct cggccgcaag cccggccgcg tattcccgca ccggagccgc gtcctcgccc
    19681 gtgcgcaggt gcgccacaag aacgacgcgg cgcagcagct ccctgtacgc cgcccagttg
    19741 cggaaccaga gcagcatatc gctgcccatg ggaaaataaa gctgtgcgcc gggataccgc
    19801 gcgttcatct gccgcacggt atcaatggta tagcttttgc cgccgcgcgc gatctccgtg
    19861 gtatccagca ccagctccgg aaacagcggg cggaagcact cgcacatggc gagacggtgg
    19921 gcggcggatg tgccgcccgc gcgcttatgg ggcggcgtac ccgccggctc caccagcaca
    19981 aggtccggcc gcaccgcttc gatggccccg gccagcagcg tcatatggcc gttgtggggc
    20041 gggtcgaacg tgccgccgaa cagaatgatc ttctgcggtc ccatggctcg cccctatctt
    20101 atttcagcag atcggcatat ttgctttcct tttgcttctg cttgtacagc acaaatttgg
    20161 agccgatggc ctgtacgcag tcgctgcccg ttctctcggc caggatggcg gcggcttcct
    20221 ttgcagaata ggcgctgttt tccagcacct tcattttgat gagctcccgc gcggcgaggc
    20281 agtccgccgt gcttttcacc agagcgtcgt cgatctcgcc cttgcccacc tggaacacga
    20341 cgtcgaggcc gttggcctgt gcgcgcagga tcgcgcgctg tttgcttgtc aacatatact
    20401 ttatcccttt tctgtatcag atttgcagcg aacggttcac ggcttgccgc gcgcgtgttg
    20461 gaaaagcccg gcgcgcccgg catttgcgcg tcagccctcc gccttgtccg ccaccgtgat
    20521 gcatgcgttc ccgcacgtca tgccgatgac gctcccatcc gcaaggaaac gcgccagctc
    20581 tgcttccatg cgctccatgg cgcggccccg gtggctgatg gcgtcttttt ccgcggccga
    20641 aagctcggcg taggagcggc cctgcgtgtt ttccgccgtt cccccgccgc ccggcacgcc
    20701 cacctcgtcc gggatgaaca gcgggtcgta cccaaagccg ttcgttcctc tgcgcacaaa
    20761 gcccacgcgg cccgggcaca cgccctcaaa cacggcgcag cgcccgccgg gcaggcacac
    20821 gcagatgctg ctgacgaacc gcgcggcccg ttttccctcg ggcacacccg ccagttcctc
    20881 cagcagtttg tcgttgttgg cctcgtcgtc gccgtggcgg ccgcagtagc gcgcggaata
    20941 cacgcccggc gcgccgtcca gcgcgtccac gcacaggccg gaatcgtccg cgatggtggc
    21001 tgtgcccgca gcctcgcaga tggcccgggc cttgatgcgc gcgttttcgg caaacgttgt
    21061 gccgttctcc tcgggctcca gcgtcacgcc ggcctcgcgc tggctccgga cggtgtggcc
    21121 catgcgctcc agaatgcggc gcatctcggc catctttcca ggattgttgg tcgctgcaat
    21181 gaattccatg ctccgtcctc cctcattcct cgcgcaggaa cagcgcgtcc acaaaggcgt
    21241 ccgggtcgaa ttccatcagg tcgtccatgc cctcgcccac gcccacaaag cgcacgggca
    21301 ggcccagctt cgccttgatg ctgatgaccg cgccgccctt ggccgtgccg tccagcttcg
    21361 taagcacgat gcccgtggcc ccggccgcgt cgataaactg gctggcctgg ctgatggcgt
    21421 tctgccccgt gatggcgtcc agaaccagca gcgtctccac gctggcggtg ccgcatgcct
    21481 tgtgcaccac acggctgatt ttggaaagct cgtccatcag attttttttg ttgtgcaggc
    21541 ggcccgcggt gtcgcagatg accatgtcgt acccccgggc ggcggcactc tgcaccgcgt
    21601 cgaaaagaac ggcggcgggg tccgcccctt cattgtgctt cacgacaggc acgtccgcgc
    21661 gttcggccca aacgcacagc tgctcggcgg cagcggcacg gaaggtgtcg cctgcggcca
    21721 gcatcannnn nnnnnncgct tgccctgctg cttgtacaga tgagccagct ttgcgatgga
    21781 ggtcgttttt cccacgccgt tgacgccgat gaccagcacc accgcgggcc tgccggaaag
    21841 ctgcatctcc ccgtcggcgc gcagcatctc gcggatgatg cccttgaggg cctccagcgc
    21901 gtccgcgcct gtcttgaggt gcttgcggcg cacctcctgg cgcagctcct ccaccagccg
    21961 catggccaca tccgcgcccg tatccgccag gatgagctgc tcctccaggt cgtcgtacag
    22021 ctcgtcggtg atctcgcccg cattcacaag gcccgcgatg gagccgaaga tggaatcccg
    22081 cgttttgcgc agtccctcgc ccagtttttc aaaaaagccc aatcgtatcc actccgttca
    22141 taaatatccg caaggattgc cccgctccgt caggaaacaa ggctcgcgtc cacatgctcc
    22201 aggtccagct tgagcacctt ggacacgccg tcctcctgca tcgtcacacc gtacagcacg
    22261 tcggcctctt ccatggtgcc gcggcggtgg gtgatgacga tgaactgtgt ctcgcttgaa
    22321 atgcgccgca gatactgtgc aaagcgcgtg acgttcacgt cgtccagcgc ggcctcgatc
    22381 tcgtccagca cacagaaggg cgcgggattc acaccgaaaa tggcgaaata aatggagatg
    22441 gccaccagcg cctgttcgcc gcccgacagc gccgacaggt tgcggatgat cttgcccgga
    22501 ggcgccacct cgatgctgat gccgctgccc agcacatcgg cctcgtcctc cagatacagc
    22561 cgcgccgtgc cgccgccgaa cagctcggca aaaatacggc cgaagttgcg gttgatggcc
    22621 gcaaagctct cgctgaacat ggcgcgcatc tcgtccgaaa ggcccgcgat gagcttttgc
    22681 agctcggcct tgctggtttc cacatcgccc acctgggcgc gcaggaactc gtaccgctgg
    22741 cttacctcgg catattcctc gatggcgctc acgttcacat tgcccagagc cttgattttg
    22801 ccgcgcacct cggacacctg gcggcgcagc tcggccccgc tctcaaacgg cacgcacagc
    22861 tcctgcgcgg cggagagcgt cagctcgtat tcttcccaga gctttgccac cgtctgctcg
    22921 tactcggcgt ccttctgctc cttctgctcg gtcaggcggg cgatctcacg tcccagcgcc
    22981 tcgcgctcgt ccgtgatctt gcgcacccgc gccgtctgct gggtgatgcc gccttccttt
    23041 tccatgcggc ggcgcaccgc tccggccttg gcctcttccc gcttttcgat ctcgttccgg
    23101 ctgcccgcaa tggctgcgcg gatggtctcg atctgcgcgg cgcgctcggc gttctgggcc
    23161 gtcagcgtct cgatgtttgc ggcaagctgt cttgcccgcg cgtcgctctc atccgtgcgg
    23221 ctctccagct gggcaatggc cgcgcggtgg ctctcgatat ccttttgagc ggccagcgcc
    23281 ttcaatttca agtcggaaag ctcgtccgca aggcgcgtgc gcgtttccag gaaggtgtcg
    23341 tcgctgccgg agatggcgcg cagttcctcc tccaacgcgg cgctgtcccg tgtaagcacc
    23401 tccatctcgg cagcggcgtc cgcccccgcc ttttcgctcg ccgcgatctg cgcggcaagc
    23461 tgggcgcgct cggcggaaag catttccccc gcggcccgcg cctgggacag cgcggcggcg
    23521 atgcgcccgc tttccacctc gccgcggatc ttgtcgcccc ccgcggtgat ggcctcgctc
    23581 tcggtggcgg tcagctcggc ggaaagcgcg tccacctcag ccttcgccct gtcggttttt
    23641 tcctccgcgg cgtcccgctg cttttccagc gcctccacct ttttcttcag ctcgtcgatc
    23701 tcctgcttgc ggctgaacag cccggcggag cgggaaacgc tgccgcccgt gaacgagccg
    23761 cccgcgttga cgacctggcc gtccaccgtc accacacggt tgcggtaatc cagcgcgcgg
    23821 gccgtgcgcg acgcctcgtt gatgtcgtcc accacgacga tgcgtcccag caggctggaa
    23881 acgatatttg catatttttc gtcatacgtc acaaggctgc tggccagcac cgcgccctcg
    23941 ggcaaacggc cgttgaagct gccgggcttg acggtgtcca gcggcaaaaa tgtggcgcgc
    24001 cccgcgcgct cgtcccgcaa aaaggccatg gccgccttgg ccgccgtctc gttttccaca
    24061 acgatgttct gcaaagcaaa gcccagcgcc gtttcaatgg ccacctcgta ccccggcttg
    24121 acggaaagga tggtggacac cggccctgtg atcccgcgca gacggcggtt cgccgccgcc
    24181 ttcatcacgg ccttgacgct gctctggaag ccgtccatat tgcgctccag atccttcagc
    24241 atggcgatgc gctgccgggc ggcctcgatg gcgcgcagga gcttctgttc gttctcgtcc
    24301 gcctgcgcca gcgcgccgcg gcggctttcc accttgagcg tcaggccgcc cttgatgttc
    24361 tcaagccgtg tcagccgctc caccgcgtcc tgcagaaaag cgtccgtctc tgccttctgc
    24421 tcctccaggt cccgcgtcag ggcggcgttg gccgcgccct cgctctcggc ggcggcaagg
    24481 cgtttttttg ccgcctcaac cgacgacgcc gcgcccgcgg ccgcgacctt taaatccgta
    24541 atgcgcgcgg ccatggcgtt cagccggccc gttaccacgc cgcgccgctc gccgggagcc
    24601 gcgctcctgt tctgcagctc ctccagcagg gcttccagct ccccggtgcg cgcggcgtat
    24661 gcggccgcct gccgctcgca ggcggcgatg ccctcgccgt ggccccgcac ctcggcggcg
    24721 atggcctcgc ggcccaggcc gctctggccg atctcctcct tcagcgacgc aatggtcgca
    24781 tcgttgtggg caatgtcgtt ttcaagcacc gcgatgcggg aatccgcccc ggcgatctcc
    24841 tccgttatgg cccgtatttc cgcgttgcag cggtcggctt cggtgatcag atgctcgatc
    24901 tccgcgcggg tgctctcggt ctcggcgtca atggcctcga tctcccgccc ggcgcgggca
    24961 tagtccgcgc ccgcgatctc aattttgcgc tgctgctcgc gcaccgtgtc ccgcgctttc
    25021 tgcacggtgt ccacccagag cgttacctcc aggcccttgc ggcgctcggc cagctccaga
    25081 tactgctttg cctttttgct ctcccgctcc agcgggccca cgcgctcctc cagctcgccc
    25141 aggatatccc gcaggcgcac cagattttcc tcggcggagg ccaggcggcg ctcggcctca
    25201 tttttgcggt agcggtattt ggcgatgccg gacgcttcct caaaaatttc ccggcgctcg
    25261 tttgattttg cgcccacgat ctccgcgatg cggccctggc cgataacgga atagccgtcg
    25321 cggcccaggc ccgtgtccag gaacagctca tagatgtcct tgagacgcac attctgcccg
    25381 tttacagaat attcgctgtc gccggagcgg taatacttgc ggccgatcgt cacctcgtcg
    25441 ttgtccacgt cgatgcgcct gtcggtgttg tccaccacaa ggctgacgct ggcaaagccc
    25501 atggcaccgc gctgctgcgt gccgccgaaa atgacgttct ccatcttgcc gccgccgcgc
    25561 agctgcttgg cgcttgtctc gcccagcacc cagcggatgg cgtcggatat attgcttttt
    25621 ccgctgccgt tggggcccac cacagcggtg atgcctttgc cgacggtaat gcgcgtgcgg
    25681 tctggaaagg atttaaagcc ctgtatttca agcgccttca aaaacatgga aaaactcctt
    25741 acttacgagt atagggacat tttgcccggc gcggcaggcc ccgcgtcaat accccatcag
    25801 ggccagggct tccttcgccg cctgctgctc ggccagcttt ttgctgtgcc cctcgccctt
    25861 gccgatgcag ttggaattga ggtgcacctc caccacgaaa tgcttgttgt ggtccgggcc
    25921 ggattcgtcg gtcaccacat agcgcaggcg ctcctccggg ttctgctgca cgatctcctg
    25981 cagctttgtc ttgtaatcct cttcggcggc ggcgtccgtg agcgcgccgg tgacaaacgg
    26041 caggatgaac gcccgcgccg tttccatgcc gccgtccagg tacagcgccg cgatgaccgc
    26101 ctcgaacgcg tcggacaaaa tgctcgggcg gcagcgtccg ccgccgcgct cctcgcccct
    26161 gcccaaacgc agataatcgc ccagggatat ctgctttgca aattcaaaca gcgccgcctc
    26221 gcacacaagg ctggcgcgca tgcgcgtcag ttcgccctcg ggcatgttct tgtgcgtgaa
    26281 caggtattcg gccaccacga tggacaacac cgagtcgccc agaaattcca gccgttcatt
    26341 gtggcgcacg ccgtgctttg cctcgttggc gtaggaggta tgcgtcagcg ccgtcaccag
    26401 cagcgccggg ttcttaaaat gatgatgaag agcctcttcc agctcgtgtg tctcgcgcat
    26461 cgttccagat ccttcctttg ttgcggatgg cggccgtccc gccggggctg ttttgcgaaa
    26521 gcgcgccgcg cgcctttccg gcggccgtta cgccccggct tcctctgtct gggccacggc
    26581 ggccagcttt tcgcgcatgc tctccaccgc gccgctttcc acgcacagct ttgcctgccg
    26641 gacggcgttt ttgaacgcct ttgcattgga cgagccgtgg gccttgatga cgggcttcgt
    26701 cacgcccatc atcagcgcgc cgccgtgttc gtcggcgtcc agctttttct taaaatcctt
    26761 catgccgccc tttaccatca gataagccag cttgcttttc aggtccgcca tgaacacgtt
    26821 tttgatctcg cccaccagca tcttggccac gccctcggtg agcttcaaaa tcacattgcc
    26881 cgtaaagccg tcgcacacaa caacgtccac attgccctgg ggcacgtcct tgggctcaac
    26941 attgccggca aaacggatgt ttttattata cttcaaaagc cggtgcgcct caacataggc
    27001 cggggtgccc tttgtctcct ccgcaccgtt gttcacaagg cccacgctgg ggtttgcgcg
    27061 gcccatcact ttctccatgt acacgctgcc catcgtggca aaggcgctga gcatctcggg
    27121 ccggcattcc acatttgcgc cggagtccag cagcagatag ggctgcttcg cgcccggaat
    27181 gacggtgccg atggccgggc gcttcacgcc cctgagccgc ttgacgatga gcgttgctcc
    27241 cgccagcagc gcgcccgtgg agcctgccga aacgaaggcg tcgccctcgc cggcggccag
    27301 catgcgcagg cccaccacca tggacgaatc ctttttgccg cggatggccg ccgtaggctc
    27361 gtcgcacatc tcaatggtct cggaggcgtc ggccacggta atgcccgcca tatcgatgcc
    27421 gttctcccgc gcgcaggcct cgatgtcccg cctgcgcccc acggccgtga tctgcacgcc
    27481 gtattcccgc acggccgcgg ccgcacccag cagaatttcc tttggggcgt tgtcgccgcc
    27541 catcgcgtcg atgatgatgt tcataacgtt ccctcccgat tgactctcat gattttttgc
    27601 gctctgctca ataatcaaag tataagggag cgcgtattgg tgcgaaagca accgctttgt
    27661 tttgcgctca gcccagatac cgctccatgg cggccacggc ccgctgcgcg acggcaagat
    27721 accgctggcg tttgtcacgg atgcgcgttt caggcgcggg cagaaggccc atgttcgcgc
    27781 ccataggctg aaaatccttc gtgggcgttg tgatataacg cagcagcgcg ccgcacatcg
    27841 tgtccgccgg cggcggcgtg aacgcgcggc cggacagccg cgcttccatg cttcgggccg
    27901 caagcaggcc gcaggccgcg ctttccatat agccctcaaa gccggtgatc tgcccggcaa
    27961 aaaacacgtt ggggcacgcc ttcagcgaaa gatcggttcc cagcagcgcc gggctgttta
    28021 aaaacgtgtt gcggtgcatc acgccgtagc gcacgaactc ggcgtgctcc agcccgggga
    28081 tcatgcggaa cacacgctgc tgctcgccga atttgaggtt tgtctggaag cccaccagat
    28141 tgtacatact gcaggccgcg tcctcggccc gaagctgcac gttcgcccag gggcggcggc
    28201 ccgttttggg gtccgtcagg cccacggggc gcagcgggcc gtagcgcatg gtgtcggcgc
    28261 cgcgctttgc catcacctcg atgggcatgc agccctcgta cacggaaggc tcgccgtcaa
    28321 aatcgtgcag gggcgcgcgt tccgcccccg ccagcgcggc atggaaagcc tcgtagcccg
    28381 ctttgtcgaa cgggcagttg aggtaatccg ccgtgcctcg cccgtagcgc gacgccgcga
    28441 acaccctgtc ccggtcgatg ctgtccgccg tgacgatggg cgcggcggcg tcgtaaaacg
    28501 agagcgcgcc ccgccccgtc aggcgggcga tgtcctgcgc cagcgcgccc tcggtgagcg
    28561 gcccggtggc cgtcagcacc agcgtgtccg gctcggcttc gcccacggcc tgcgcctcgc
    28621 ccgtgcgcag cgtgatattg gggtttccct tcacaagcgc cgtcacgccg gcggcaaagg
    28681 cctcgcggtc cacagccagc gcgccgcccg ccgccacgcg gcagccctcg gccacgggca
    28741 gcagtgcgct gcccagcagc ttcatttccg ccttcagcag gccggaggcg gaatccagcc
    28801 ggtcggcctt gagggaattn nnnnnnnnnt ggagcacaca agctccgcga aatccgcgtt
    28861 tttatgcgcg ggggaaaatt tatgcggctt ttgctcccac agttccacgg cgtagccgcg
    28921 gcccgcgaga tacagcgcgg cctcgcatcc cgcgaggccc gcgcccagga cccggatgtg
    28981 catgctctta ctcctttttt tcggcaccca gcgcacggga gtaaccgcag ccctcttttt
    29041 cgcacagcag gataccgttc ttt
//
LOCUS       DAWBSB010000003        28164 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_461_length_28164_cov_3.576079, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000003 DAWBSB010000000
VERSION     DAWBSB010000003.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 28164)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 28164)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..28164
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_461_length_28164_cov_3.576079"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    892..901
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3336..3345
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3879..3888
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8460..8469
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    20544..20553
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5269 a         8277 c         8953 g         5615 t
ORIGIN      
        1 gctggtgacc tcggagcagg tgccggaggt ggtggggatg gcgaccagtt tggccttctg
       61 gcccagcttc gggaacttga acgcacgctt gcgaatgtcc aggaagcgct ggcgcaggcc
      121 gtcgaagctg gtgtccggat gctcgtagaa cagccacatg cccttggccg cgtcgatggc
      181 cgagccgccg ccgatggcga tgatgacgtc cggctggaag gcgttcatgg ccttgacgcc
      241 gcgcatgatg cactcaacgg acgggtcgct ttccacgtcg gagtagatct cgctgtggca
      301 gtattcctcg cgcttgcgca ggtagtacag gactttgtcc acattgccca gcttcaccat
      361 catcgggtcg gtgacgatga aggcgcggga gatgccctcc atcttttcca ggtactggat
      421 ggaatcctcc tcaaaataga tgcggggcgg caccttgaac cactgcatgt tcaccctcct
      481 cttggcgatg cgctttttgt tgatgaggtt caccgtcgtg acgttctggg acacgctgtt
      541 cttgccatag gagccgcagc ccagcgtcag ggaaggcgtg ttggtattat agatgtcgcc
      601 gatggcgccc tgggaggacg ggctgttcac aatgattcgg cccacgcgca tggccacgcc
      661 gtattcatcg gcgattttca tgtcgccggt atgaatgacg gcggagtggc ccaggccgcc
      721 cagctccaac atcttgttgg cgtaatcaaa gccctgctgc ttgctgttta caacaaagta
      781 ggccagcacc ggggaaagct tttccttcgc cagcggatgg ttttcgtccg gcgaggagat
      841 gggcgcgatg aggatcttcg ttttttcggg caccttcagg ccggcctgct cnnnnnnnnn
      901 ngctcggcga tccagtttgc agatttgccc accagcgggc cgtaaacgcc cttgctgggg
      961 tccgggaaca tgaatttcgt cagcttttcc gtttcttcct tgttgaggaa gtagcagttg
     1021 ttggccttca tatagtcttc gaacgctttg gcgatctctt tgtccacgat gacggcctgc
     1081 tcggaagcgc agatcatgcc gttgtcgaag gtcttggaca tcatcaggtc ggtgcaggcg
     1141 cggtgtaggt ccgcggattt ttcaatatag cacggcacgt tgcccgggcc gacgcccagc
     1201 gccggtttac cgcagctgta agcggccttt accatgccgg ggccgcccgt ggccaggatg
     1261 gtggccacgc cggggtggtt catcagcgcc cccgtcgctt cgatggacgg cgtgtcgatc
     1321 cactggatgc agttctccgg cgcgccggcc ttcacagcgg cctcatatac gatgcgcgca
     1381 gcctcggccg agcacttctg cgcggacggg tggaaaccga agatgatggg gttgcgcgtt
     1441 ttcacggcga tcaggctttt gaacagcgta gtggaggtgg ggttggtggt gggggtgacg
     1501 cctgcgataa cgcctacagg ctcggccact tccacatagc cctccatctc gttctcgtcc
     1561 agcacgccca cggtcttttc ctttttgatg tcgtgccaga tatactcggt ggcaaacata
     1621 ttcttgatga ccttgtcctc gtacacgccg cggccggttt cctccacggc catgcgggcc
     1681 agctcctggt gcttgtcaag gccggccagc gccatctcgt gcacgatgtg gtcgatctgt
     1741 tcctgatcca gcgtcaggaa ttccttcaaa gctgcctggg ctttttccac caggccgtcg
     1801 atcattttgt tcacggggtt ctccacggtc tccacagcgg gggccggagc ggttttggcg
     1861 gtcgtctttt tctctgccat cagtttgtcc tcccaaagtt cattgatatg gtttgaacgg
     1921 ttgttttgtg attttaggat gctttctcac tgacactatc ataccatatc gttaaaagtt
     1981 tatcaatgta caacacgacg aatgttataa gtttaacaaa gttcttttgt gcattctaac
     2041 aaaagaagag gaaagaagat tgtgaaaaat ttctgcagaa ccggcacaaa atgcgggata
     2101 taccgccggc ttgtttcgtt tttggatgta tttgtgtata atgaaaagaa aacggcgcct
     2161 tacggcgcaa aaataaattg caggcgcgcc aagcgcaggg agtaaaaagt tatggaatgg
     2221 acagacatca agacgacggt gccggcggtt tgggccgaaa cggcggaggc cattgccacg
     2281 ggcatctcgg gcggcggcat ctacatcgag gactaccggg atctggaggc ccaggtggag
     2341 gccattgctc acgttgacct cattgagcag gaactgctgg ataaaagccg ggatgaggta
     2401 gcggtgcacc tgtacattgc gccggacgag aattttgcgg agatcggaga actgatgcgg
     2461 gatcgcctgg agcgcgccga ggtgccttac cggatggagt acacgggtgt gcgccaggag
     2521 gactgggaga cgggctggaa ggcatactac cacgctatgg atatcggcac acggctggct
     2581 gtggtgccga gctgggaagc gtacgagacg gaccgcgcgg tcatcacgct ggacccggga
     2641 atggccttcg gcaccggcac gcacgagacc acggccctgt gcctggaaac gctggatggc
     2701 ctggtgaagg gcggcgagcg cgtgttggac atcggcacgg gcagcggcat tcttgccatt
     2761 gcggcattga aattgggcgc ggccaaagcg gaaggcgtgg acatcgaccc gatgtgcgtg
     2821 cgcacggcag gcgaaaacgc ggccctcaat ggcgtgcaga acgaccttac ggtactggtg
     2881 ggcgaccttt ccgaaaaggc cacgggtgta tacgatgtca tctgcgccaa catcgtggcc
     2941 aatgccatca tccacctagt gccgaacgta cccgcgctgc tggcaaagca tggaaccttt
     3001 ctggccagcg gcatcatcga cacccgcaag gatgaggtga tcgccgcggt acaggccgca
     3061 ggccttgcac tgcgggaggt aaaggaaaag aacggttggg tggcgctggt gtgcggccac
     3121 acagggcggg aataactgcg cgccaagcac aaaggcggaa ataccgtgca tacgccgggt
     3181 gctaaattat ctgccaccgg gctgaaatgg attgcgtatg cctgtatgct gctcgatcat
     3241 tttgcatacg cctttttgta ttaccgctct cctgcatgca ttgcgctgcg aaccgccggc
     3301 aaaatggcgc tgcctatttt ctgctttctt ttggcnnnnn nnnnnttgcc acaccaaaag
     3361 cgtgcgccgc tatttgatgc ggctgctgat gtttgcaatg atttcgcagc cgttttacag
     3421 cagcttttgc ggctgagtgt atacgcgcga atggaaattt acggtatttt cgggcggagg
     3481 caatgtgttg tttacgctgt ttcttgcgct gctttcgctt gtggcatggg agcacatcaa
     3541 gccgttgttc gcacggtgcg ccgcttgcgc cgcccttttc gtctgcgccg ctcttcttcc
     3601 tttggattgg ggctggtatg cgttggccat tgtttggggc tgcttttaca tgcgcgaccg
     3661 cagcggccgc ccggctttgc tgctgtgcgg tatctttacg gtaattgtcg cgtcgctttg
     3721 gcttggcgcg ctgcccaatt tgggctcgca gtatctgcaa tttttcggtg tgtttttttc
     3781 gcttttgcca atgcggctgt acgacggcac gcccggcgcc ggtgcgcgct tgaagtggag
     3841 gttttacctc atctatccgg cgcagtttat cttaattcnn nnnnnnnntc ttaattccgc
     3901 tgcttggcat gcggcttgcg ccttaaacag cgcaccgcag tgccgcgccc ttgcgccata
     3961 aatacacaga aggagtttta ttatgcccca ccgttatttt acaactgata tctccggagg
     4021 cacggccgtg ctgtcggggg cggacgcgca tcacctggcc catgtgatgc gtgcccggcc
     4081 cggcgagacc gttacgcttt gcgacggcaa gggcacggac tatgcctgcg cggtgcgcgg
     4141 ctttgccccc gatacggtag agctggaaat cctttccagc tctaccagcg tatcggagcc
     4201 gggcgtgcgg gttacgctat atgtgggtta cccaaagcag gataagctgg agacaatcgt
     4261 ccagaaggct gtggagcttg gcgccgtgcg ggtagtgccg tttttcagcc gctactgtgt
     4321 ggctgcgcca aaaaaggaag agcagaaaaa cctgcgctat aaccgcatcg cctttgaggc
     4381 cgccaagcag agcggacggg gcgtggtgcc gcaggtggaa cttccggtaa catacaaaca
     4441 gatgctcgcg gacgcagcgc gcaacgacgc ggcgtttttt tgctacgagg cgggcgggga
     4501 gccattactt agccggctgg gcggcgccga gaccatcggc ctcatcaccg gcagcgaagg
     4561 cggcttttcc cccgaggaag cggccgcggc ccgcgaagcg ggatgcgtac aaatcggcct
     4621 ggggccgcgc atcctgcgtt gtgagaccgc gccgctggcg gcgctcgctg cggtaatgac
     4681 cctgaccggc aatttacaat gaccttcccg cgcgccgccc ggcgtctgcg ggcaggcgaa
     4741 agggagtttg tattttgcct aagaaaactt tggtccttat cctcgcgctg gtgcttgtgg
     4801 cggcgctgtt cgcaggctgc tggggcagct ccggcagttc gtcctccaag ccttcgtcca
     4861 gctcctccat ggcgcccagc tcctcgtctg tgacatccgg ctccggcagc atgtccggtt
     4921 ccatgtccgg ctccatgagc ggcgttcccg gcgacagcgg cgctgcctcc ggctccgttt
     4981 ccggcagtat gtccggcagc ggcgcgggcg gcgcttcttc ttccggctcc atgagcggtt
     5041 cctcctcggc tgcggcaccc gccgcagccc cggcggacgg cgacggctgg atgacgcgcc
     5101 ttgtcaacgc cgggaaccct ctgcccgagg gctttaccgt ggagaccgtg gacatcaggg
     5161 gatacgaaaa ccgtcctttt gacaagcgcg cggcggcgga cctggaggcc atgctggcgg
     5221 acgccgaggc ggcgggctgc aagctctact tagtgtcggg ctaccgcagc gtggagcggc
     5281 agacggcgct gttcaagcgc aagacaaatt ccttcatggc ggagggcttc agccgggagg
     5341 aggccgaaaa acaggctgcc atgtgggtgg cgcgtcccgg cacctcggag cataacacgg
     5401 gccttgccgc agatatcgtt tcggcggact ggtattcgaa gcacagcgac ctgacggcgg
     5461 attttgaaga cacgccggaa tttgaatggc tgtacgccca ctgcgcggac tacggcttca
     5521 tcctgcgcta ccccagggga aaagaaaatg tcacgggcgt gacgtatgaa ccgtggcatt
     5581 atcgttatgt aggtaaggat gccgcgaaag ccatcatgca ggcgggcggc accctggaag
     5641 aatacaccgg ggcgcatacc gcctgatgcg gcggcgcgcc cgggaaaacg gggcgtggat
     5701 acgagtggcg ttcataaaaa aatacgcggg cacggtattg ggcgcggcgc ttctgctggc
     5761 gctggccgtg ttcggcatta catatcgaaa cgagatctgg gccgtgctga cgcagcagga
     5821 tgcgcgggat gcgtttattg cgtttgtcca cgacagcggt tttttgggcc ttctggcatt
     5881 tttgggcctg caggtgctgc aggtggtggt ggccgtgctg cccggcgaac cggtggagct
     5941 gatggcgggg cttttgtacg gtacctgggg agggctggcc ttgtgccttt tgggcgtggg
     6001 cctttccagc gccgcagttt attactgcgt gcgtgccgcg ggtgccagag ccatcgacgc
     6061 ttccgttctg gcaaaatacc attttctgcg ggacgaggcg cacgtgaagt tctttttgtt
     6121 cctgctgttt ttcatcccgg gcacgcccaa ggacgtgctc atctatctgg gcccgttcct
     6181 gcccgtcagg ccccatgcgt tttttctcat ctccacactg gcgcgcatcc cgtcggtgat
     6241 caccagtacc tttgcgggca gccagttcgc cgagggaagc tgggacgtga gcctggtggt
     6301 gttccttgcc accggggctg tggcggggct gtgcgtgatt tttcaggacc gcattctggc
     6361 aggcattgcc gcgctgaagg ggaaattcag tctggaaaag cacggggatt tgtgatatac
     6421 tgcctgtgga tttcccagca gagagctggg gactgatttt ccagtaaaca taaaggagcg
     6481 acaacgatgc gtgaatataa gatcctgacg gattccacca cggacctttc gccgcagctt
     6541 gtggcgcaga cgggcgttac tgtactgccc atgacctata ccatcggcga aaaaagctat
     6601 cggaacgacc ctgaggaaac agacctttct tccggggact tttacgatat gctccgcggc
     6661 ggggctatgt ccaccaccag ccagatcaat gtggagacat tccgggaagt ggcgggtaaa
     6721 atggcgcggg acggcttcga cgtgctgtat gtgggttttt cgtccgggct gtccggcaca
     6781 ttcaactccg cgcgcatcgc cttcgaggac ctgagcgcag aatatccgga gcacaaattc
     6841 ctcgcggtgg acagcttgtg cgcctccatg ggcgagggcc tgttggtcta ccatgcggcg
     6901 cagcagcaga aggcgggccg cagcattgaa gaaacggccc ggtggctgga ggaaaacaag
     6961 ctgcatctgg cccactggtt cacggtggac gacctgaatc atctcaagcg cggcgggcgt
     7021 gtgtccggcg cggcggcgtt tttcggcacg atgctcaaca tcaagccggt gctgcacgtg
     7081 gatgacgcgg gtcatctgat ccccatggaa aaggtgcgcg gccgcaaagc cagcctggac
     7141 gcgctggtgg cgcatatgga aaaggacgca gtggacccgg cctcccaaac ggtgttcatc
     7201 agccacgggg actgccgtgc ggacgcggaa tatgtagcgg acctggtgcg ggaaaaattc
     7261 ggcacgcagg atatccgcat ccattccatc ggcccggtca tcggcagcca ttcaggcccc
     7321 ggcacagtgg cgcttttcta tcttggccgc acgcgtggat aaaatacttg aagtataaac
     7381 cggatttaaa ataacgaaac cagcccccgg ccgcgcagga tagacccgcg gtcgggggct
     7441 ggtttgtatt cgcaaaatat gcacggaaat acctgaggcg gcgggcagag ttcctcattg
     7501 cgcgcgttcc cgttttttgc tatgatggga acaggaatca tctgcttgcc gcgggcggtt
     7561 tttcgcggcg gaaaaggggg aatgcagcaa tgagcgcaca ggaaaaaacg gcgggcgggc
     7621 tttcgctccg caacctctgg gcgtatcccg tgggcaccgt gggccgggat atggccggat
     7681 gcatgtttac caactatctg ctcacctatg tgttgttcac caaaacgctt acgtcggtgc
     7741 agtttgcctg catcagcgtc atcatggtgg cggcgcgcgt attcgacgct ttcaacgacc
     7801 cgatcatggg caacgtggtg gaggccacgc gcacgcgctg gggcaaattc aagccatgga
     7861 tcgccattgg cggcgtgctg agcgtcatcg tgttcatcac ctcgttttcc accacgcttc
     7921 agggctgggc ttatgtggcg gccttcggct tgttgtactt cgcgtacagc gtcgtattta
     7981 caatgaacga catcgcctac tggggcatgg tgccggcgct ggcgcgccgc gccgacgacc
     8041 gcaaccgtct tacctcccgc acggtgctgt ttgcgggcgt ggggcagttt ctgggcggca
     8101 tcgttgtacc cacgtttacg gcgggcagcc ttgtgatcgg cggcaacgcc gtcaccgcgt
     8161 accgtgccgt tgtccttatt gtatgcgctg cgtttacggc tacacagctc atcacgctgc
     8221 ttgtggtgcg cgagccgtct gcgcgggacg aagcgcccgc cgaaaaggtc ggcatccgga
     8281 aggccttttc caccattgcc cgcaacgatc agcttgtctg gtgcgccgtt gtttttctga
     8341 ttttttccgt ggcgcagaca ctgatgggcg gcggcctttc ggtgacatac ctgtattttg
     8401 aattcggcta caacggcctg ctgctgtcca tcttcagcat tctgggcaat gtggcgggcn
     8461 nnnnnnnnng cgcggtgctg atgctcgtgt tcgcggctct gtccggccgt ttcacccgcg
     8521 cgcagctgat gaaggcaggc gctttcggcg ccgcggcggg atacctctgc atcatgctct
     8581 ccggcctgtt catcccgcgg gagatgtggg cgctcaagtt cacgatgctc accgtgagca
     8641 acctgtttgc atttctgggg cagacgctgg tttaccttat tatgatcatc tgcattgcaa
     8701 ataccgtgga atacaacgag tggaaaacgg gccgccggga ggagggcatc gtcttttccg
     8761 tgcggccctt catcaccaag ctgggcactg cgctggtgca gcttctggtg cttgtcatct
     8821 atctggcggt gggagtgcgc gccgtgacga accagatctc cgatctggag aacgcggcct
     8881 cccaggggct gatcaccata gcggaaaaga cggaacgaat cggggcggtg ctggcctcgg
     8941 tgcccagcgg caaaagcgcc gcgctgctgg tctgcatgac ggtcatcccg gcggcgcttc
     9001 tgttggctgc ttatttcatc taccgtgtca aatatacgat cacggaagaa tcttatgaag
     9061 cgatggtgcg ggatattgag gcgcgccgcg cggcgaaagg agaaacagcg gaatgaaagg
     9121 ctggaaaacg gcactggcgc tgggcgccgc ggccgcggct gcgggagctg tggccggaga
     9181 gtacttttac cgctttgcgc ttacacagca aatcgacaaa acagcggcgt tcaacgcgcc
     9241 gcataaccgt cccaccggcg cgccccgggg cacgctggcg gcagacaggg aatggctgaa
     9301 cgcgcagccc catgaggatt ggtggctgac ctcgggcgac ggcctgcagc tgcacgctct
     9361 gtgtgtgcgc gcgggcggga atgcgccctg ggcggtgctg tgccacggct ataccgggca
     9421 ggcgtcgggc atggcggaat acgcgcgccg gttttatgac gcgggcttca gcctgctgct
     9481 gccggacgca cgcggccacg gccaaagcga gggcgggtat atcgggatgg gctggcccga
     9541 gcggcgggac attgccgcat gggtgcagcg tgttaccgca gagaacggcg cgccggatat
     9601 cgtgctcatg ggtgtgtcca tgggggccgc caccgtaatg atgacggcgg gcgagcctct
     9661 gccgcccaac gtgcgggcta tcgtggagga ttgcggctat accactgcat gggaggagtt
     9721 ccgttatcag ctgaaaaaaa cctatggcct gccgccgttt cccgtgctgt acacggcaga
     9781 cgcgctgctg cgccgccggg cgggcttttc tttgcgggag gccagcgctg cggcacaggt
     9841 ggcgcactcc cgcacgccga tgctgttcat ccacggcggg aaggatgcct tcgttccctt
     9901 tgccatgctg gatgaggtgt atgcggcggc gcggtgtccc aaggaaaaat atgtggagcc
     9961 ggatgcaggc catgcggagt ccctgcgcat ggcgcccgcg cgctattggg caaaggtgtt
    10021 cggattcatc ggccgctgga tgccgctgcc cggcgcggag cggacagcgg cggcggcccc
    10081 ggcggaaaag ccggtataag gcgtcgcata cataaaagaa aaagcagcgg agaacaggcg
    10141 gcatctgcct gttctccgct gcttttgttg tgacatacag cttgaacaca aagaaggaaa
    10201 gcatatcccg gtgcttaaaa cagggcggag acgccgtcca cgactccgga ccgtgtgaaa
    10261 ggatccggct gagcgaaagg atggccgttc cggtcggtga gggcggcgcg ggccgcgtcc
    10321 tccatacgct tgtaccggat ctccacggag agcgggccgg gcatgcggta ggggacgagc
    10381 gtcccggcgc cgggtacggc ggcccgcacg gtgcggtaga tgtcgtcgca cacagcctgg
    10441 ggatgccggc tcaaagcaga cgtccaggag agcgcctcct tcgtcacaac ggcggcgagg
    10501 ccgggaaaca gcgcacgggc ctcggccaca caggcgtcgt cgcccgcgca gaacagcacc
    10561 ggaacgccca gctcgcccgc gtatgccgcg tcgatggcaa gctcgcccgc ttcttttccg
    10621 tccagcttat aatattggaa cgcggtggaa cagaaggtgt gggccaacac ggcccgggag
    10681 gtgttttccc gcgcatgata gccgatgaac agcacggcgg tatagctttt gtccatgcct
    10741 gcaaagcggc ctctgtgccc gctgcccagc aggatgcggc agcgtgggtc cagcaggtcg
    10801 tactgcaggt ttacgcctgt gccgtgcgcg tcccagacga cgacctcctg cgcgcctgcg
    10861 tcgaacaaag cacgggccgc tgcgttcgcc tcccgcgtgg cctgcaggca ggcgaaccgg
    10921 tagttttctc cgctgcccag gccctttccc ggttcgccca ccacacaggc ggggccttcg
    10981 cagtctaccg cgatataaaa tttcatggcg gcactccttt ttccttcact tgaagcgtca
    11041 aacgttcaca gcttcagcag ccccagcccc gagccgagcg gtttgccttg cagcaaaagc
    11101 tcctttgcgc cccgctgcag agacgtgcgc tcaggcagcg cggcgtccgg gatacccggt
    11161 gcgccgaaca gccagagacg gccggaattg tcatcaggat cccagtctgc cagcgtgtaa
    11221 tcctccataa aattgcgaat gccggaaccg ggcgggagca gctccttcac gcggagcgaa
    11281 agaagccatg tgcggcaata ggtcaacggc aaagcgccgt cgcgggcaaa aaagtccgca
    11341 cgccgcgcat aggaggcatg gagcgcttcg ggcgtacaca tcgcatcgcc gggaatatgc
    11401 accgagagcg cgggcgcgcc cgccgccgcg cccaacggcc cggcaagctc agcgggcagg
    11461 ggaagcgcct cgaattccag tgtgccgagg cggagcagcc gcccgcagag ctgccgccag
    11521 acccagaagc cgcggtcgaa ctgccagcgg cctgtccagc ggtgcgtctc gttcagaaaa
    11581 cgggaaaagc agcccatcgt ggcaaggtag acatcgtcgg gaacgccccg cgccgcataa
    11641 tgtttgcgcg cctccaatgc ggcggacagc agcacggcca gctccagcag cccattggga
    11701 cacagcggcg cgcatttcgc ctgcagttca cgcatggcgg cttcggcccg cgatgcgtcc
    11761 agcagggcgg ggatatggcg cagcaagggc gccgcctgtg ccgaagcggc ctgcgcaagg
    11821 caggcttcgg cggcggggcc gggaatcgaa agctcgatgc acagcgcgtg cagggccggg
    11881 atgtcaaagg cttccatggc tgcctccttg tttgttgtgc aaaggccggc ccggcccctt
    11941 cgttgtaaag ggctttccgc cgggccggcc tgcggcgccg gttccggccg cccttgagca
    12001 cggccggaaa agcgcgttat ttttcggtaa actcggccag ctccactgcg gagccgccgt
    12061 gtgtgcggga gtgctcggcc gccagcgcca tcacgtggct ttccaccgag gcgtccaccg
    12121 aggtgaggcc ttccgcaccg cctgcggcga taagctcgca cacataatcc atcatcagcg
    12181 cgtcgccgcc gccgtggccg gcaaagcggt cggggatggt gcccaggtcc agaacctgct
    12241 cgggctggcc gaatttgcgc aggtacagta cgttggcgtc catatcgcct tcgatctcac
    12301 ccatcgtacc catcaccttg atgctgcggt agcattttgc cgtgaaggcg ctcatggtga
    12361 aggtggctgt cgcgccgccg gcaaagcgca tggagacgac ctggtggtcc accacgtcgt
    12421 tgtcacaccg gtatacgcac cggccgtagg gccctgtgcg cagcgcgtca tacagctttt
    12481 cctcggtggg tttattcacc agcaccgagc agggccactc gtcattgccc gaacggatgc
    12541 cgcttttgtc attgaaaatg tagatctttt ccgcgtcgta ggggcatcct tccttacagg
    12601 cgcagccgga aagacagcgg tctgcactgc catccggcgc gttttccgcg cggaaccagt
    12661 ccagagagcc gtaggaggaa acgctttcgc agggaacgcc catcagccag cggatgatgt
    12721 ccatgtcgtg gcagcttttt gccaggatca tggggctgga ttcctccgcc cgccgccagt
    12781 tgccgcgtac atagctgtgg gcataatgcc agtaagccac gttctccacc gcgtccaggg
    12841 attcgatacg gccgatggcg cctccgcgca gcagctcgta cagcgtgccg tagaatttgg
    12901 tatagcgcag cacatggcac accaccacca cgcggttcgc ctcatgcgct tttttttgca
    12961 gtgcaaggca ttcgtccagc agcggagaaa tgggtttttc cagcacaagg tgatagccct
    13021 tgtccagcgc ggccagcgcc tgcgccacat gctggcggtc ctgcgtggca atgatcatca
    13081 catccgcaag gcgcggctgg gccagcagct cttcgcctgt ggcaaagcag cttccggcgg
    13141 gcacgccgta attcgcctgc agtgcggcgc ggcggtcggg gtccgggtcg gctccggcgg
    13201 tgatcttcat tttttcaggg tgctgcttct ggaaggcggc atacgcctcc agcccgcgga
    13261 tgcccaggcc gcagatggca aaggtaacgg gtttgtccat ggtagttcgt cctttctttt
    13321 tttgcggata acagcgcggc cggatgcggc gcgcttagaa cccgtattca cagaacggaa
    13381 cgccatctat ccggcggatt ttgcgccctg ctgcgttaaa tttccttgaa atagcgctgc
    13441 tattcctgcg aaaatttgcc ttgcatggca caaaatccgc tcgaatatcc gacattcctt
    13501 atttaagaat acagattctt agatatcgag cttgcaggcg gagacgatct cccccaccgc
    13561 gtagctgccg aacagcgtgc cgtccgggcg cagcgtataa ggcaaggcgg gagcggaaag
    13621 ctcggggatg tccgcgcaga cgcccgcggc aaacgcgcgc atgcgcgctg cggcggtgga
    13681 aagccttgcg gccacgccgc ccaggcggag gtcgatgatc tcgaagccct ggggcctgtt
    13741 agtggcttcc cacagcgcgc gccatgcggt gcgaagcgcc tccgtcgccg caatggttcc
    13801 gggcagggct tcggcaaggc gtgccgccag agcaagatcc tttcggcgga cggcctgcgc
    13861 ggcctgctcg tgccaggcgc atttgccggc cagcacgcgg gccaaaaggg agtaaaaacg
    13921 gaaaagaggt tcaaaggccg ggttttcatc ggcataccgg gtatagcgcg cctcaagctc
    13981 cgtataatgc gcgctcatgg cgagcccggc ggtgtcggcg gcaaagagct gcaccagggg
    14041 gtcctgatac agcaggaatt tggccgcgtt gacaggccgc agcgcgcccg agcgcatgcc
    14101 gggcacggcg ttgaacagcg aaaggtccaa aaacgcctgt gcgtctgcac cgcagcagca
    14161 ggcaaaacgg cgggcgagag agccggcatc gtatgtgccg gtatacatga attcggcgta
    14221 cagctgcatg cccagcagag cggacgtgag attggcctcg gcgccgttgt cgccccaggc
    14281 cgtggccagc accagcggca cacccgcttt tttgcaggcg gtcagcgctg ggacggccgc
    14341 agccagcgtt ttggcgtaat ccggcgcagg cccgcaccat gtccagatcc cgcccgcgaa
    14401 tacggtgggc gcgggcagtg ctgcgtgctt ttgcagcata tcggtatact cctgctcggt
    14461 ctcgtggtaa tagtcccaat acacaagcgt cacatccggc ggcacggcgg ccactgcttc
    14521 ggccgagggc ataccgctgt cataatagcc gtcggtgggg gaatcgggac ggaaatacat
    14581 atcgctccac atcatggccg aaaggccgtg gcggcgtgtg atctccagca cacgggaaag
    14641 atgccggcgg atgatggtgt gggggtcctc gtagccgtgg cggcgcaggt gcgcgcccaa
    14701 accgatgccg tgggcctcgt ccatgccgat gtggatgcgt ttggaacggt agggcgcggc
    14761 ggcagccgcg ataagctcct ccaggaatgc gtaggtttgc gcatcgtcgg ccagcagaac
    14821 ctcctcgttg tccttcagat gcgccagcgc gggccagtgc agcgcgcggt tcaaatggcc
    14881 cagcgtctgg atgcagggac aaagctcgat gcccagcata tcggcataat catccagcgc
    14941 ccgcagctcg tctgcggaat aacgcccgcg ctgatagccg aagtagggct ggccgggcac
    15001 ctcataggtg tcctcggtgt acatcatgcc caggttgagc cccatcatgg ccattttgcg
    15061 caggaaaaac cgcagcgtgt ccggctgcag caccgcgttg cgggaaacgt cgaacatcat
    15121 gcccgttgtt tcaaagcagg gcgtttcctc aatgcagaaa gcgtcctccg cccagtgctg
    15181 gcgcagcagt gaaagtgcgc ggtatacctg tacgggttcg gcccaggtga ccaccacgcc
    15241 gtccgcacgc ttttccgcgc gcaggcagcc gccctgacgc agggctacgc tcaggcctcc
    15301 ctgtccggcg cgggcaaagc ccagctcggg gccgatctcc gcaaaagcag cgtcaaaatg
    15361 ctccggcagc tggccggaga aggagagaat ttcgttcatg gccgtctcct ttccctgcac
    15421 gcggcaggct tacagcaggc tggcgctgtc cgcgtccaga tacagtgtcg cagcggggtg
    15481 gcgccgcagg atgctggcgg ggcaggcggt gcggatgtcg cccagcagcg tgtcgcgcac
    15541 ggcgcgcgcc ttggtggcgg cgggcaccac acagaacacc tgggcagcgt gcatcagtgc
    15601 gggcactgtc agcgtcagcg catgggtggg cacgtcgtcc agagtggcga agcagccgtc
    15661 atgcacctgc tggttgcggc atatctcgtc cagcgccacg ggctttacca gggcggggtc
    15721 gtcgaaatcg gccacggggg ggtcgttgaa ggcgatgtgc ccgttttccc cgatgcccat
    15781 gcaggtgatg tccacgggga aacggcgcag cagctgggaa tagcgggcac actcagctgc
    15841 cggatcccgg gacgccgcgg tcccatccag gcagttaacg gatcgaaagg gcaccttggt
    15901 gaagatggcc gcccgcagaa aattggcaaa gccctggggc gcgtccggcg caaggccgat
    15961 gtactcgtcc atatgaaagg cgttcacacg gttccattca atgcccggct gcgccgcaag
    16021 cgcggccagc atctcgttct gcgagggtgc ggcggcgaag atcatattac actcttcctt
    16081 ctgcgccaat acgccgcgga tggcggcggc ggcttcgtct gcggcgcaga agcccatttc
    16141 cgcgcgggtg tcgtagatct ttacgcggaa ctggttgatg gaaatggttt ttgtcattgg
    16201 aatgcctcct ctcggttttc aaaacacacg gcgccgtcct tgaacaccat ggcgatgcgg
    16261 acggtatggt cgaacacggc aaggtctgcc gcgcggcccg cctccacgcg gccgtaaaaa
    16321 tcggaaaggc ccagtacgcg ggcgggggtt tcggtgatca tacgcactgc atcgggcagg
    16381 ctggcgccgg ccagcgtcac cattgtgcgg accagcctgt cggctgtgca gatgctgccc
    16441 gcgaaggccg tgcggtccgg cattttggcg acgccgtcct ccaggatgac gcgctggccg
    16501 ttttccaggc tgcccaggat gctctcgccc tgtgtctgtc cggcgccgcg catggcgtcc
    16561 gtgaccagca ccagccgctg cgggccgatg tgtctgtaag ccagctgcag caatggagcg
    16621 ggcaggtggc agccgtcggc gatgatttcg ctggacagct catcgtacag atacgcactt
    16681 tccacgatgc ctgcgtggcg gaagccggcc ttgcgcacga tggtggacat ggccgaatac
    16741 aggtgcgtca catggcggta gccgcaggga atggcggcac gcaccgcttc gctgtcggcc
    16801 tcactgtggc cgatgctggg cagtacgccg cgcgccagca gcgcaagccc catctcccgt
    16861 gcgccgggca gctcgggcgc gatggtccag cgaaggatgt gcgggcaggc gtcaagcagc
    16921 tcgttgtatt cggcggggtc cggctcgcgg atgaagcgcg ggtcctgtgc gccgcactgg
    16981 cttttggcaa tgtaaggccc ctcgatgtgc aggcccagaa gcttcgcgcc gtccgcaaag
    17041 ccgtcccggc agcgttcaaa tacagaaaag gagcgcagca gctcttcctt tgaggcggcc
    17101 agcgtagtgg gcaccatggc cgtggtgccg tggcggaggt gcagcgcgct cgcgccgtga
    17161 tatgcctccg gcgtgccgtc cataaaatcg tgtccgccgc ctccgtggac gtggatgtcg
    17221 ataaagcccg cgctgacgta caggccggcc aggtcatata cgaagtccgc cgccgcgccg
    17281 ctgtccggcg gcaggacggc atccacagcc ttgccgcgca gcagaatatc gcagcgggtg
    17341 atgcagccat cctgcaccag gcgcccgccg cgcaaaagtg ttgtgctcac agcagcccct
    17401 ccttttgcag aatgtccttc agcgccgcga cgctctcggc cgtggccgcc gcaccgcccg
    17461 ctgaaaagcc cgcgccgccg ggcaggcgca tttgttcctc cgtcaggtgg gaatccagac
    17521 ggtaatgggt ctccatggac agccagcccg catagccgtc cgccgcaagg cggcgcagca
    17581 gagcgggata gcccacctgc cccgtgccca cctttacgca gtagctttcc ggcacagcgc
    17641 gcaccgcgtc cttgatgtgc acatgggcaa agaagggacg gatggcctcg tagccgtccg
    17701 gatagggcgt ttcgcccaag gggtcgtaca ggcaattgcc cgggtcgaag attgcgcgca
    17761 cacggggcga gccgatggct gtgacaagcc gggccagcgc cgcatggttt gaggtgttga
    17821 cgctggggtc cgcctccagc agcagcgttt tgccgcgctg ctccagcagg cgtacgggcc
    17881 gctcaaaata aggcgccagc gtttcggcgg gcaggggccc ttcctccggt gcgaagaatg
    17941 caaagccgcg gatgaacgcg cagcccaaaa tgtccgccgc ggcgcacagg cgttccagct
    18001 tggccagctc ggcttccaca gagtccggtt ccggcgcgca tttgtaaaac gaaccggcca
    18061 ggccgcaaac ggtcaaaccc tcggcgtcca gcctgcggcg ccatgtgcgc agcgtttcag
    18121 ccggaagcat gtcgatggca gtgtcctcca cgctgcgcag ctccagccct gccaggccgt
    18181 tctgccttgc gaaccgtacg gcctcgtcaa agctctgcgt cacctcgtcg gtgatgaatg
    18241 agattttcat aagaacctcc atgtgccgcg tcccatagct tgaaaaggaa cgcggcacgt
    18301 cgtttttaca gggatgaggg gaaaacgcgc gggcttagaa aacgatgggc ctgcccgtgc
    18361 gggtggattc gtaaatagaa cagatgacgc gcacggccag cgcggcctcc tgcgggggaa
    18421 tggcaggaga acggccgtcg cgtacggcct gtgcgatatc gcgcagcagg cagatgtggc
    18481 cgtaattgcc gattttcgtc gggtcgcccg caccgcccac gcgttcgccc atatcggggc
    18541 gctgaggcgc gtcggcctcg tcgaggaact tccactccag tatgccctcg tcgttgaata
    18601 cgacggtgcc cttttcgccg tgaatataaa aggtggacga aaagcccgga tatgccgtgg
    18661 tgcagccctg gatgacgcag ataccgccgt gcttcatatt cagcacggcc gcggcggtat
    18721 cctccacagg gatgtcgcgg gccagcgtct cggcgcggcc gtacagcgtg gagacctcgt
    18781 cgcccagcat ccacaaaata aggtcgatgc catgcacgcc ctggttcatc agcgcgccgc
    18841 cgccgtcctg ctcccaggtg ccgcgccagc ccgcgctgtt gtaataggcc tggtcacgat
    18901 aatatttcag gtccgctccg gccaggcaga tcctgccgaa cctgccctcg gccacggcct
    18961 gcttcacagc gatggccaca ggcatcatgc gccgctggaa gatgcactgc atcttcgcgt
    19021 gggtgcgctc caccacgccg atcacatccg cgatcttctc cggcgtgatc tccataggct
    19081 tttcgcacac aatggcctta ccggcttcgg cggcggcttt gcacacctcg ccgtgggtgc
    19141 cgctgggtac gcagacgcat acgatgtcca cggcagggtc cgccagaacg tcgtgataat
    19201 catagtagac gtggggcacg ccgtgttcgg cggcataagc gtcggccttt tccgggatga
    19261 tatcgcacgc gccgtacagc acgcagccct cgccgcgcag ggcctccagc gctttggcat
    19321 gggtgaaggc gatcacgccg cagccgatga tggcaaaatt catactctca aatctctcct
    19381 tttcgccgtg caggtcacac ggagttctga tttgatggta ctcccttttg ctcaaaagag
    19441 atttgcatat catcccataa agttggcaaa tcgtcaaaac ataggcgctg tgtacaaaaa
    19501 aagcagcgtc cgtgccgggg cagggcgctg ctttcggaga atatcagtac agtctgcctg
    19561 ccgccgcgcc gtagcccctg cagaaggcac ggccgtcgta agcgggcatt gcgtccgtca
    19621 gggcatccag cgcaacgggc gcgcctccct gcgcgcggga ggccgtggcc gccagcatta
    19681 ctttgacgga ttccagcagt gcgtccggcc ccgccagaag attgggcttg ccctccatat
    19741 aattgcagat ctcgcacatc agcgcctcgt acaggcggtt ggagtccatg cggaaatggt
    19801 gggtggtttt ggtcgtcata atggtgagaa caaagggctg ccaggtgcct gtgaaggtgt
    19861 tgaaaacagc gctttttccg ttttcgtact gtacgaaata gctctcggag tacttgccgc
    19921 cggcttcgcc gcggcccatg tagcgcacgc tctgtgcgcc ctgtcccaaa atgccgccga
    19981 tggcctcggc aatgtgtacg ccgtagttga attcgtccac gccggcagtg ccgaacacgt
    20041 tcaccacctc gccgcgctcc tcttcaggca gcgccaggaa ctgctgcact tcgtaagcgt
    20101 agcgcgcgct ggacgcgccg aggatcaccg cgccgtcttt tacaagctgt tccacgcggc
    20161 ggcagtcggc cagatttccc accagaggct tgtcgatgaa cacaggcttg ccctgctcga
    20221 tgaaaggcat tgcgcagcgc agatggtcgt cccagtcgca gccgtggatg aagccgatgt
    20281 cgcacatcgc ggcaagctcc tccagcgtat tgcagcgctt ttccaagccg aagcggcgga
    20341 tgaagccctc cacctcgctg tccggacgga aagagtcgtt gtacacgccc acatagcgtg
    20401 cacggtcgtc cttttccatg acctcgccga atcccagagg gtgggaggtg tcgatgttga
    20461 cggcgccgat gcgtatcata aattcagcac ttccttttac gggccgccgc acaggcggct
    20521 gttgttttgt ttgcattata gggnnnnnnn nnngcagcag ccttaccaca ggctgctgcc
    20581 gccctgccgc tgaaagttga aataacggct ctgcccgttg cgcatgcggt attccatggg
    20641 cgtgcagttt tccttcgctt tgaaaaagcg gctgaaatag tggatgctct ggaagcccac
    20701 aaggtctgcg atctcgctga cgctggtgtc ggtgttcacc agaagctcct tggccttgcg
    20761 caggcggata cggttgatgt agcggatggg cgtggtgccg tacacctcct tgaagatgct
    20821 gatgagcgtt gttttgttga cgcccgccag ggtggaaaga tcctccaggc tgatgatgcg
    20881 ggaatagttg aaatcgatat actgcatcag tgcggcaacg tccacaccct tgaacgtggc
    20941 gacgcggggc gcctcctcct cgggcgctgc ggcgggctgg cccagccgtt cccgcacgat
    21001 acgtaccagc atttccagca aatagccgct gatgatggag taataatagg ggcgctgctg
    21061 ggcgctctct gcgatgatct tgtcaaaaaa gcgcacgaac catccgaaat cctgcaaatg
    21121 gaacatatcg cccacgcgca ccagatcctc gaacagcgcg ggatccgcca cggtgaattt
    21181 tacatccagc attttcagcg gcgcagcgtt tttttcaccg cagtggatgg cgtgcagctg
    21241 ctgggggcac agcagaaaca cctgctcctt ttcgatctca taaggctggt cgtccacata
    21301 cacggtgccg ctgccactca gaatgcctac catttggaaa tagttgtggc agtgcagctc
    21361 gttttcccag ttttggtggt acttgccgca ccagaggatg ctgcattcgt tcatccgtgt
    21421 tccccttcct ttcactgctt ttctacaata tcagtataca gcaacgcctt gcgcaaaggg
    21481 aacacctgcg aaaaaatagt acaatattcc taaaaaaagt gtaaagacac cggcgggagg
    21541 cgacggtata atgtgggcaa gagggccggc cggacaacgg gcggcggaaa aggagcttac
    21601 cgaacgatga aaattgccgt gctgatggac gcgccgctgc gcgcgacgct gctttccgaa
    21661 aaaacaatgg cccgcctggc cgaatggggc gaggtggccg tgaacgaaac ggacaaggcc
    21721 gaaaaagaga ccgtcaagca gatcattgcc ggggcggata tcgccattac atcctggggc
    21781 gtgccgcagc tggataagga aattttggac tgcgcaccca acctgcgctt tgtggcccac
    21841 gccgcgggca gcgtgaaggg catcgtcagc gacgagctgt atgcccgcgg catccgcgtt
    21901 gtcagcagcg cgcgcatcct cagctacggt gtgtcggaaa cggcgctggg ccttaccatc
    21961 tgcgcctgca aaaacgtgtt tgctttcaac gaggagatcc gcaacggcgg ctgggtggag
    22021 gattacagcg tcatcaccga gctgttcgac atcaccgtgg gcgtggtagg ctgtggcttt
    22081 gcaggctccc actacatcga gctgctgcag gcgttcggcg taaatgtgct ggcctatgac
    22141 ccgctgctgg acgaagccgc catcgccgcc atgggcgcga aaaaggcaga tttggatacg
    22201 ctgctggccg aaagcgatgt ggtttcgctg cacgcgcccg cgctggattc cacccgccat
    22261 atgatcaacg gccgcgcgct ttctttgatg aaggaccgag ccatcctcat caacaccgcc
    22321 cggggcgccc tggtagacga ggaagcgctt gcgcaagcgc tgcgcggcgg caagctgaaa
    22381 tatgcctgcc tggatgtgac ggaccccgag ccgcctgccg cggacagccc gctgcgcggc
    22441 atccccaact gcatcatgac gccgcatctg gcgggccttg ccaacaacgg caagctgaag
    22501 attggcgcgc acgtggccga cgagatcgga cggttcctta caggcggggc gctggtgtct
    22561 gagatcacgc aggccatgct ggccaccatc gcgtaagcgc catggaacgt atcgttaaca
    22621 cagagctcgg cgtcatacgg gaaccgctgc gcgcaccttt cggcttcaag ggaggcacgc
    22681 tgagcgagct gtggcagacg gccgtgcgcc tgacgctgga cagcggagcg agcggcatgg
    22741 gtgtgggcgt acagagcgtg ctctggtccg accccgtcac attctgcgcc tgtgacgaag
    22801 cgggcggcaa cgaaagaatg ctggcggtca cgcgccgcgc gctggagctt ttgcagggac
    22861 aggctttcac agacccgccc ggtatgaccg cgggcctggt gccggagctt ctggcgttcg
    22921 cccgcagact tctgggacgg gcagacgtgc cgcaaacctt tgtactcaac gcgctggtac
    22981 ccgtggattt tgcgctgtgg cagctttgga acgcaaaacg ggggaacggc accttcgacg
    23041 cgctgtgcgc gtccttctgc ccggagctga cgaaccggga aaaagagttg gggagcatcc
    23101 cgctcatcac ttaccatacg ccggaggacg agctgcacgc cctgctggag gacggcgcct
    23161 ttttattaaa ggtaaagatc ggttccgacc cggagaaaaa cggagacccg gaggccatgc
    23221 ttgcgtggga catcgggcgc ctgcgcaccg tccacacggc agccgccggc tttaccacgc
    23281 cgtatacgga gtgtggccgc cccgtctatt atctggacgc caacggccgt tatccggaca
    23341 gggaatttct gctgcgcttt ctggatgctg cggacaagat ggacgcgctg gagcgcatcg
    23401 tgctgctgga ggagccgttt gcgggggacc ggccggaacc cgtgcatggc ctgccggtac
    23461 gcgtagcggg cgacgaaagt gcccacagcg cacaggacgt tgcctgcctg acccgggaat
    23521 acggctattc ggccatcgcc tgcaaaccca ttgcaaaaac gctgtccgtc acgctggaga
    23581 tggtgcgcgc ggcgcagaaa agcggcgcgg cgtgcttctg cgcggacctg acggtgccgc
    23641 ctgcgctttt ggactggaat atgtctctgg cggcgcggct gccgcgtgta ccgggcctgc
    23701 gtgtgggtgt tgtggagagc aacggcccgc agaattatac cgattggcgc cgtctggacg
    23761 ctatgtgcgc cgcgcccgga gccgcctggc gcacaccgca gaacggcgtg taccggcttg
    23821 acggaggttt ttatggggaa agcggcattt ttgcgccgct gcccgcttat gcggacacgc
    23881 tgcgcccggt ttgaacagga gggccgcgcc cggaaaggaa tgttgaacct ttggatttga
    23941 aagtgcattt tgccgtggcg ggctgcggcg tgatctcccg gttccatctg gacgcgatcc
    24001 gctccatcga ggacgcggcg ctgtgcggcg tgtatgaccc tgtggaagca agcgcccgtg
    24061 ccgcggcaca ggcttatggc acccgggcgt atccggatta cgacgcgctt ctggcagacc
    24121 cgcaggtgga cgcggtgtgt atctgcacgc ccagccacct gcatgcgccg ctggcactgc
    24181 aggctgtggc cgcgggcaag catgtgctca ttgaaaagcc cgtggcgctg acgttggagg
    24241 actgcgacgc tgtggccgcc gcagcacgcg ccgccggtgt tcaggtgggg gtagtctccc
    24301 agctgcgtta cggcccggcc gtggcgcgcg taaagcgcgc cttggagagc ggggcgctgg
    24361 ggcgcatcac gcgctgcgat ttgtatatga aatatttccg cccccagagc tattatgatt
    24421 cgggtacctg gcggggcaca caggcgatgg acggcggcgg ggcgctgatg aaccagggga
    24481 tccacggtgt ggaccttgtg cggtatctga tggggccggc agacagcatc tatgcgctga
    24541 gcggcacgct ggtgcgggat attgaggtgg aggatacact cacagctgtg gtggcctggc
    24601 actgcggcgc gcagggcgtc attcaggcgt cggtggggga ttaccccggc tttccgcgcc
    24661 gcatcgagct gaacggcgag tcaggaacga tcattttgga agaagaccgc atcgttaaat
    24721 gggaggtggc gggagactcc gcttaccata tttatgagca ggaacccgcg ccggaggtgc
    24781 gctcccacag tgaggcgggc gcgattgacc cttcggggca tgtggcacag ctgcgcaact
    24841 ttatcgacgc ggtgcgcggc gaggggacgc tgctggtgga cgaggccgaa ggccgcaagg
    24901 cactggcgct ggtgctggcc gcttaccgct ccgcggccga ggggaagccc gtagttctgt
    24961 gacgctttta ccagtaaaac tgctcggcga cggttctgtt ttggatgttt tttcaaattc
    25021 ggtgggtcag gattgaacga tttatcaagt ttgcctgcca aatgcggcgt tttgaaattt
    25081 aattttgtgt aaattgtttt tacgaggcga aggacgcagc aaagccgttt ttaggcggaa
    25141 ctgcccgcgg cgcctcaaaa aaagtgcaaa aaaggccgcc cggaaggcgg cggccgaaaa
    25201 tggaagattt gccaagaaga atttttgcag atttggtata gtataggagg gcaaaaaagc
    25261 acccggggca catcgacgcg cgcttttgcg caaaagcgcc ggtgcctgga gccaacggct
    25321 ccaggatatt cttttaaaag agacccgtgt gcacgcccgg aattgatgcc atgtacgggc
    25381 cgggctgacc ggtcgtccgg cccgttcgga aataatgttt catcaggagg tcacttatga
    25441 aaaaggcact cgctttgatc ctttctcttg cgctggctgt ttcggcgctc accgcctgcg
    25501 gcggcggagc atcttcttcc gcagccggca gcacatctgg ttccgccccg gcatccgccg
    25561 cgccctccgg cgaaaagacg gtggtcaccg tctggcacat gtatgccgag gatgaggaag
    25621 taaccaagcc ccatcagcgt ctgctgcagt gggccgagaa ttacaacgcc accaacaccg
    25681 ataacatcga agtcgttgtg tccggcgcca aaacggcgga cgtcatcatg acgacgatcg
    25741 cttccggctc tacgccggac atcttccaga actactggaa caacgcgccc acctgggcga
    25801 acaatggcgc tttgtacgac cttaccgagt ttgtcaacgg cggcgacgcc gagtggaaca
    25861 aggacgattt catcgacgcc gcatgggatg tgtgcaccta cgaggataag gtatattcca
    25921 tccccttcac gtacagctct acgttcctgt tctacaacaa ggacatgctg gccgaggccg
    25981 gctgggaaga attccccaaa acgatggacg agctgatcca gtgcattgac gactgcacga
    26041 aggttggcgc tgacggttcc attgagcaga tgggcctgat ccccgattat ccctggctgg
    26101 acaacgttct gtggcctgtg gcgttcggtg cgcagttcat cgacgaagag accaacacca
    26161 tcaccttcga ttctcccgag atgctcaagg cttatcagtt ccaggccgac atttacagca
    26221 agtacggcta tgacaacgtg aagcgcttca tcgattctct gggcgcccgc gccacaaccg
    26281 aggacccgct gttcactgga aagctggcca tccgctggca ggcggactcc gcgctggctt
    26341 ccatggtgga tgctgcgaag gagaccggca ccaacatggg catcgccccg atgccggctc
    26401 ccgccgaggg cggcgaaggc cagggcatgc tgagctgcgg cgtgtgggaa gtgaacgcca
    26461 aaacggcgaa cgccgaagcc acgctgaagg tgctcaagag cctgaccagc gccgagaaca
    26521 tggccttcat ggctgagggc gactatggca acggcgcgtt catgccgcgc aagagcgctc
    26581 tggacgcggt catcgcgatg gacggcgttt ccgaagaggc caaacaggtg gctgagatgc
    26641 tgcgcgacgg cgagttccgt gcgttcccca tgagctccta tgtcaacgag tatctgactg
    26701 agatcagcac ttacatgacc gaggcgctcg ccggcaatat gaccgtagaa gacgcggcca
    26761 aggccgttgt ggaagcggtg cagcccatgg cggacgaggc cgccggctga acagacaaaa
    26821 gaaacagtaa agaatagagg aaagcgccgg ctgtgcggtt acccggcccg gtcggcgctt
    26881 tccttttttg tatcgcgcct gtattcccaa agcccgtgcc gcgggccatc tctacgaaag
    26941 cgaggttttg gcatgagaaa tgcatcggcc gccaaaaagg aaggaaacgg gatcaaaaaa
    27001 cgaaaatcga aacaagagcg gaggttcttc ctccttggcc tgctgtttgt ctcgccgtgg
    27061 ctcatcgggt ttttgtgctt ccagctttat ccgatcctct ccgcgctgta ttatagtttt
    27121 acggatttta acatcttcca gccggcagag ttcacagggc tggacaacta tgtcacgctg
    27181 ttcaacgaca aatattttcc ggtgtcgatc aagaacaccg ccgtcatgtc cttcatcggc
    27241 atgcccatcg gccttgtggt cgctctggcg ctggccgtgc tgctgtcgaa ggaggtcaag
    27301 ggcatgcccg cgttccgcac catctattat ctgcccacag tggtgcccat tgtggccagc
    27361 gccatgctgt ttttgtgggt gctcaacccg gaatacggcc tgctgaacaa ctttttgtcc
    27421 tggttcggca tccgaggccc aagctggctg agcgacccga agttcaccaa gatctcgctg
    27481 atgattatgg acgtgtggcg ctgcgggcag aacaccgtca ttttcctttc ggcgctgaag
    27541 gcggtgccaa agtcattcta cgaggcggca gagctggacg gagcgggccc tgtcaagcgt
    27601 ttctttaaga tcactctgcc gtacatttcg cccaccatcc agttcctggt ggtgatgggg
    27661 cttatttcgt cgttccagta tttcacgcag gcgtatgtgt tcgccagtgt ttcccaggtg
    27721 gggcagagca tcactggcgg gccgcagaac tcgcttttgt tctattcgat gtatctgtat
    27781 atgaacggct tcagctacat gaagatgggc tacgcctcgg ccatggccat cgtgctgttc
    27841 ctgattgtgc tggtggtttc gttcgtggcc atgtacctga tggaaaaacg cgtcacctac
    27901 gacgtagagt aaggggggaa cagctgtgaa aaaatggatc atctctgcaa aaacctcgcg
    27961 caccgtgggc aaggtctgct cttatattct gctgatcttt tttgcctgca tctttttcat
    28021 gccgttcctg tggatgcttt ccaccgcggt aaagcctacg aaccagatat tcctgaatcc
    28081 gcccaagtgg attccggacc ccattaagtg ggaaaatttt gcgacgatct ttaagcgtat
    28141 gccgctgttc gcgtatacga agaa
//
LOCUS       DAWBSB010000004        27532 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_479_length_27532_cov_3.812534, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000004 DAWBSB010000000
VERSION     DAWBSB010000004.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 27532)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 27532)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..27532
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_479_length_27532_cov_3.812534"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    7657..7666
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    17404..17413
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    21584..21593
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    25923..25932
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5355 a         8300 c         8604 g         5233 t
ORIGIN      
        1 catctttgcg cagcaaagtg caagccacgc ggcgaatgcc gccaaaaagg ggtggcgaac
       61 ccctttttca tttaaaataa atccgccccg tgcgcggcgg attttaaaca ggaggtttgc
      121 ccatgcgcgc gattctgtac ccaagtgaga tatccggccg cgtggccgcg ccgccgtcca
      181 aaagcatggc acaccgtgcg ctcatctgcg cggcgctggc caaaggcgaa agccgcgtgt
      241 tgaacctttc cgcctcgcag gatatcacgg caacgcttgc cgccatgcgc ctgctgggtg
      301 cgtccgcggc ggatatggac gcgccggagt gcaggcgcgg cgccgcggcg gcctatgccc
      361 attggagcgg tgagcgcact gtctccggcc cgggctccct tatcctaaaa ggaacgggcg
      421 gcctgccctt tgcgccggtg tccggcccgg tggattgctg cgaaagcggc agcacgctgc
      481 ggtttctcat cccgctcttt tccctcacgg acgcgccggt acgctttacc gggcgagggc
      541 ggctgatgga gcgtccgcag ggcgtatatg cgcggatgtt tgcggagcgg ggcctcggct
      601 ttgcgcagga cgcagacggg attacggtgc gcggcgcgct gtccgccggg gaatatacgc
      661 tggcgggaaa catcagcagc cagttcatca gcgggctgct gtttgctctt ccgctgcttt
      721 ccggcgccag cgtcattcga atcacgcctc cgttcgagag ccgcagttat gtggacctga
      781 cgctgcgtgc gctggccgat ttcggcgtgc gggccgcgtt cacggatgaa aacaccatcg
      841 ccgtgcccgg cgggcaggcg taccgcccct gcgcgtacac ggtggagggc gattattccc
      901 aggccgcgtt tctggcggtt ttgggcgcgg cccggggcgg cgtggccgtc acgggcctgc
      961 gggaggattc tctgcagggc gaccgggcca tactggatat cctggcgcgc tgcggcgcgc
     1021 gcatcatccg gacggacggc gcggcgctgt ttgaaaaaag cgccttgcgg gggacggaga
     1081 tcgaccttgc ggactgtccc gatctggggc cggtgctgat ggtgctgggc gcgctgtgca
     1141 aaggtgagac ggtcatccgc aacgccgcgc gcctgcgcat gaaggaaagc gaccgtatcg
     1201 ccgccatgga gcaggagctg cggaaaatgg gcgcggagct gttttccaca caggacaccg
     1261 tgaccgtgcg cggcggcgcg ctgcatggag cggacggttt atacggccac aacgaccacc
     1321 gtgtggtgat ggcgctggcc gtggccgcgt atgcggcggg cgtgcccgcc gccatcggcg
     1381 gggcggaggc aataaacaag agctggccgg acttttttga aacggtccgc gcgctgggcg
     1441 gaaaggtgga actgacggat gaataaagag cgcaatatct gcatcgtggg cctggggctt
     1501 ctgggaggca gctacgccat gggcctgacg gacgccgggt ataccgtcac ggctgtggat
     1561 gtgcggcccg aggccatccg ttacgcgctg gaaaagggca tcatcgccgc gggcgcggtg
     1621 gaggactttg cgccgctgct ggccggagcc gatgcggtgg tgctgggcct gtatcctcac
     1681 gcgctggtgg attggcttcg aaacaaccag aagcacctca agcccggcgc gctgcttacg
     1741 gatgtgtgcg gcgtaaagag cggtgtggtg gaggagatcc agggctttct gcgtccggac
     1801 gcggagttta ttgccagcca tcccatggcg ggcaaggaag tgagcggcgt ggagcacgcg
     1861 gattgcgcga tcttcaaacc cgcgaatttc atcatcgtgc ccacggaaaa aaacacgccc
     1921 cggggccttg cctttgcgcg cggcctggcc gagacgctgg gctttgccca catcacgcag
     1981 ctcacctgcg ccgagcacga taaaatgatc ggttatgtca gccagcttac ccacgccatc
     2041 gccgtcagcc tgatgaacgc caacgacaac acccatctgg ccgagtacac cggagattcc
     2101 ttccgggacc tgacgcgcat cgcgaagatc aatgaaaatt tgtggagtga gctgtttttc
     2161 ctgaataagg aaaacctgat cgcggagatc gaccagtttt ccgccagcct ggaggggctg
     2221 aaacgtgcgc ttgtggaaaa tgacgagacg gaattgaaac ggctgttcgt gcagtccact
     2281 gcgcggcgca ggctgtttga taaaaaacgg gaggcgcgct gagatgaagc tggagcagat
     2341 gccgcgttat ccgctgctgt gcggctttac gccgctgcag cgcatggaga atctggagca
     2401 ggcgctgggc tgcggcccgc tgtacatcaa acgggatgat ctgacgccgc tggggctggg
     2461 cggcaacaag acgcgcaagc tggaatttct gttgggagac gcgctggccg ggggggcgga
     2521 cacgctggtg accgtgggcg gcgtgcagac gaatcacggc cgtctgacgg ccgccgcggc
     2581 agcgaaggcg ggccttgcct gcacgctggt cctggacggc gcgcggccgg aaaagctctc
     2641 cggcaatctg ctgctggact gcctgctggg cgcgtcgctg gtgtacacgg acggacggag
     2701 cacatcggcg gtcatagagg aaacgctggc tgcgctgcag gccgcaggca gggcgcccta
     2761 tttcatcccg gagggcggca gcaacgccgt gggcagcgcc gggtaccttg ccatggtgcc
     2821 cgagctgctg gcacaggcgg acagcctgcc ggttcccccc gcgcggctgg tgtgcaccat
     2881 gggcagcctg gggacgttcg cggggctgtg gctgggagcc agagccttcg gcgcgccgtt
     2941 ttccgtcacg ggcgtggcgg tgaaccccac aacaggatat acgagggaaa aggccgcggc
     3001 gctggtgaat gagatgagcg cggcctacgg gctggacatt gccgccgcgc cggaggatat
     3061 ggacatcatc tacgccgacg cggctcacga ttacgcaggc ccgggctaca acgtaccgga
     3121 cgcaaagacg cgcggggccg tggagctgct ggcccgcacg gaaggcattt ttctggaccc
     3181 gtgctatacg gccaagtcct tccggggctt ttgcgatatt gcgcgggagc tggacggcgg
     3241 agccatattt gtacatacgg gcggcacgcc cgctctgtgg acgcaggagc atctggacgc
     3301 ttttgcggcg gactactgga attgaattaa acacggcaga gtcataacca ccagctaagc
     3361 tggtggcttg agcaggctct ataagagcct tttaccggca ggcgtctcaa gacgcaccga
     3421 atcctctttc gtctgcatct cgcgctctgc cgcccgtaaa cgggcaattc gctttcccga
     3481 tcgcttcact cgacgcttga cgctaaagcc tttttacggg gttcctccac cggcatagcc
     3541 ggtggtttcc ttactcaaca aaagcgcccc ggcacatgtg tgtgccgggg cgctttgttt
     3601 tgaaagggat cagatctgcg tgacttcgcc gccgtgccgc gcgcgcatcc acgcttccag
     3661 ttcttcggcg ggcatggggc gcgcataaac gtaaccctgc accaggtcgc agcctatgct
     3721 gcgcagaaaa tccacctgtt ccggcgcttc aatgccctcg gccaccgtct caatgcccag
     3781 cttgcgggca agctccacga tggattccac cacgtcgtgg gcgcgctggg aatcgtccag
     3841 gaaaaagctg cggtccaact tcacggaatc caccatgaag gacttgagca gccccagcga
     3901 cgagaacccg gcgccgaaat cgtccagcga gcaaaggaag ccgagctcat gcatgcgccg
     3961 gatggcctct tttacgttca ggatatcgcc gacagtgaaa aagatggatt ccgtcagctc
     4021 cagctcgatc aggccgccgg ggatctggta ctggtcccgg atatccgcaa accgctgcag
     4081 aaaatccgtt gtctggaaat gccggcgcga gaggttcacc gcgacgggaa acaccggcgc
     4141 gccttgcgcc aggcggcccg caagcatggc gcagacctcc tcgaacacat acagatccag
     4201 ccggcaaatg tcgccgctgc gctcgaacac agggataaaa tccgaagggt agataaaccc
     4261 cttctgcgga tgctgccagc gcaccagtgc ttcggcgctg gctacctgcc cgtcccgcag
     4321 acggaccttc ggctggtaat acaccttgaa gtcccggttt gccagcgagc tgtccagcag
     4381 gttgacaagc tccttttcaa gatgcatccg cgcggtgatg cccgcgtcgt agaacgcgca
     4441 gcgcccgttc tccgcgggct tgcttttgcg ggcggcgtcc gcgcggtcct gcattacagt
     4501 gatatcgagc cctggctcct gtacaaggta ggcgcccgcc agcagcgtca gataataggg
     4561 cgtctcccgc tgggcgttga aagcgttgac gtcggcgcgt atttcctcca gccgggcaat
     4621 gacggcgtcc tcccggtgct cgtgcaggca caggaaaaag tgtccggcct caccgcgcgc
     4681 ggcggcctcg tccggccgga tatggcgtcc cagcacctgc atcacatggc gcagcgtatc
     4741 gttgccctct ttggggccga aaatctcatt gatgaggcgg tagtccatca ggttcagcga
     4801 aacaacggtg aaggcgccag gctttgcgcc atccagaaca cggcggcagg aatactgaaa
     4861 gcgcgcgttg ttcatgccgc ccgtcaccgg gtccacaaag gcgatgtgct ccagctggcg
     4921 gcgggggctg taatagatgc cgcacataac ggccaggatg agcgaaaaca gtacgatgac
     4981 cgcggtactg agccggaagg tatgctggat gtatgcatcg gtatcatggg agatgatgtc
     5041 ggcgggcagg attgtgagaa gcacccagtc aaacgtttcc aatggatcgt aggacatgat
     5101 cacgtccacg ccgtccgggg tggtaaatac gattactccg ctcacatttg cctgcatatt
     5161 ggcctgcatc tctcttacgg cagaggccag ctccggatcc ttgtttgcga caaacaggtc
     5221 gtccagggcg aagaaaaagt ccaggtttgt gggagagatg actacgttgc tgtcgcggtc
     5281 aataatacag gaaacgctgt tgccgtcaaa gccgtcggtc gcgatcagtg cctgcatgtt
     5341 ggctttgtcg cgctctccgg ccaaaacgcc gaaaatgctt ccctcgtaga cgatgggaac
     5401 ggtataaagg atgcgctggt tttccagaaa ggaaacgccg cgttcccctg ccgcggaagc
     5461 ctcgaagccc ggcaggccgc tgaaattatg cgtggaagca tcggtgcaga tgccctcccc
     5521 ctctacatcg cacaaaacaa gctgcgtaaa accaagcatc tgtgctctct gctttaaaaa
     5581 gtccatgcga tgctcagctc cgtctatccg cacaaggctg tccgttaaca tttccagcgt
     5641 ctgcatcaca ttggcaagct gtgcgtcgat attctgagcc atctggaaag tcacatcgct
     5701 cacataggcc tttgttctac ggtccacggc agtctgcagg ccgttggcgt ttaccacaga
     5761 gaagatgcac acggcggcga tgagcaccgc caagaatacg atcagcaggc cataaagcga
     5821 acggcggagc caattacggg aattgtgccg gacaggcatg ctctgacgcc ccataatcgt
     5881 gaaacctcct ttggccgcac ggcctgaaat atagagcagc ggcgcagcgc cgaatatact
     5941 ataactgtat aatatttcag tcaaaaagac aagcaattgg cagctttttt acatagaaaa
     6001 agtaaaaagc agcaccagcg cagaaataaa ctgcactggc gctgcaaaaa taaacagccg
     6061 gactcagacg ccggaatagg cggagaaacc gccgtcgatg gggatgacga cgcccgtgat
     6121 gaaggaggct gccttgttgt tcaaaaggaa cagcagcgcg ccgtccagct cctcgctttc
     6181 accgaagcgg cccatcgggg tggcggcaag gatcttgccg gtgcgcgccg tgggcgtgcc
     6241 gtccgcgttc cacagcagag cggcgttctg cttggtggag aagaagcccg gagcgatggc
     6301 gttgcagcgg atgcccactt tggagaagtg aacggccagc cactgggtga agttggaaat
     6361 ggcggccttc gcaccggagt acgccgggat cttcgtgagc ggagtgtagg cgttcatgct
     6421 gctgatgttg atgatgttgc agccctcgcg gcccaccatc tggcgcgcga acgcctgcgt
     6481 cgggatcagc gtacccagga agttgaggtt gaacacgaac tccacgccgg aagcgtccag
     6541 gtcaaagaag cttttggtgt cggcgtcgat gtcgccttct tcaaaatatt ccttatcggt
     6601 ggtggctcgc ggattattgc cgcccgcgcc gttcaccaga atatcgcatt tgccaaagtc
     6661 cttttccacg gcgtccgcca cttcatagca gacggccttg tccagcacgt tgcacttgta
     6721 agccttggca acgccgccct cggcggtgat ctcatccgcg aagctctgcg cggcttcctc
     6781 gttcaggtcc agcagggcga ccttcgcgcc cgcgcgggcc agcgttttgg caaacgcgct
     6841 gcacagcacg ccgcccgcgc cggtgacaac ggcaactttg ccggaaagat cggtatccag
     6901 tacatttttg ttcatgggaa tgacgctcct ttcaaaattc aaatggcggg cggcatgcgg
     6961 ccgcccggcg ttttggcgga atcagcaaaa gctgcgggct tcctgcggcc cattccgcag
     7021 gcttcagaca acctgctggc gcagcgggcc gcgcggcctg tacgccggtt cggcgggagt
     7081 gcaagccccg ccgggccgga aaacatccga agaagccgcc ttatttgcgg tccttctcgc
     7141 aggcttcaaa caagccgttg atataagtgg ccccaagggc gcggtcaaac aggccgtagc
     7201 cgggcttgcc cgtctcgccc cagatcatgc ggccgtgatc cgggcggaca taaccgtcga
     7261 agcccgtctc caccagcgcc ttgacgatct cgtagatatc caggctgccg cagctggaca
     7321 gatgagcgcg ctcctcaaag ctgccgtcgt ccatgatctt gacgttgcgg atgtgcatga
     7381 aaccgatgcg gcccatggcg ctgtatttgc gcaccatctt ggccacgtcg ttggatttgg
     7441 cgcagcccag gcttccggtg cacaggcaca ggctgttggc ggggctgtcc accagagcca
     7501 ggaagcggtc caggttttcc tcgcaggtga tgatgcgggg caggccgaag atgggatagg
     7561 gtgggtcgtc cggatgaatg gccatcacaa cgccgcactc ctcggccacg ggaatgacct
     7621 tcttgaggaa tttctcaagg tttttccaca ggccttnnnn nnnnnncgcg cacctcctcc
     7681 tgggagtagc tggaatccca gccgggcaga tggatgtcgt ccttcagcgg gtcaaggccg
     7741 ttcatctggt cccagtacat caccaggctg gtggagccgt ccggcgcctt tttgtccagc
     7801 tgcgtgcgcg tccagtcgaa cacgggcata aaattatacg tgacgcactt tacgccgtac
     7861 ttggcgcagc gacggatgtt ttcgcagtac acgtccagca gctcgtccac attgttgcgg
     7921 ccgagtttaa tgtcctcgtg cacggggatg ctttcgacaa catcgaacac aaggccgtgg
     7981 gcctcgcact gggccttcat cttggccaag ctctcctcgg gccacacctc gccgggcttc
     8041 acatcgtata cagcggaaac gatgctgtgc atgcccggga tctgcgagat gtactccaga
     8101 gaaatggggt cggattcgcc ataccagcgg aatgacattt tcatagaaaa aacctcccgt
     8161 tttgttttcg atacactggg gtgcgcaaag ccgcagaacg cggcattttg atacttttag
     8221 tgtaccatac ggatttacaa taaaaattgt tattattgct tgacgcattg cgaaacttgt
     8281 cttaaactaa aagcaaagga ccgctggggg acagcggcgg caccgtccat atgcggtgcg
     8341 gaaggagggc tgccggttgg cgggcaaggc acagaatttt acgcagcgtc agcacatgct
     8401 gcgccccgga tacgagatct tccgctactg cgattccgag ctgggcgagg tctcgctgca
     8461 ccatcatgat ttttacgaga tctacctgtt tctgaacggc agcgtggaat acagcatcga
     8521 gagccgcatc taccgcctgc tgcccggcga catcctgctc atcggcccca tggagctgca
     8581 ccagccccgc atcgcgaagg accacggccc ctatgagcgg atggtgctct ggctggacaa
     8641 gcggtatctg gaacagctca gcacccccca gacgagcttg acgcgctgct ttgacgcaag
     8701 cgcgcccggc cacaccaatc tgctgcgcct gccgcctgcc cagcgggcgg agagccagac
     8761 gctgatggaa cgcctcgcgg cggagagtga gggtgcgcag gccgcggcct atggcagcga
     8821 tgtggtggcg cgggcgtgcc tgctgcagct tttggccggg ctgaaccgct ttgcgctggc
     8881 ggccccggcc cgccacgagg tggaggacaa ggcgggccct gtcgtggccg aggtgctgca
     8941 ctatatcaac gagcattacc cggatgagct gtcgttagac ctgctctccg caaaattttt
     9001 tatcagcaaa taccatcttt cccacgaatt ccaccgcctg gtgggtacca gcgtataccg
     9061 gtacatcatc caaaagcgcc ttgtcatcgc aaaacagatg ctggcgaacg gcgtggcgcc
     9121 cacggacgta tacggccatt gcggctttgg ggattacgcc aatttttacc gggcgttcaa
     9181 agcggaatat catatcagcc cgaaacagtt tctgggccgg gctggaaatg aaaatacatt
     9241 tggaggttga cgcaaaatgt ttactgtgac aaaaaccact cagggcgcgt atacagccta
     9301 tgtcctggcc gataccggcg ccggcaccac cgccaccgtc gtgcccgaaa agggcggcat
     9361 ggtgacgagc ttcaccaaaa acggagatga atattcctgg ctgcgggagc cgaacttctc
     9421 tcagccggag cgcccgcgct gcgccatgcc cgtgctgttt cccatgtgcg ggcgcgcgcc
     9481 gcaggacgcg aacaccttcg gcggcaaaag ctatcctatg atcatccacg gcatcgtcca
     9541 ctccatgccc tggcaggtgg tggagacggg cgaaacggac ggcgcatccg tcaccgtgcg
     9601 cgtcacggac aacgccgaaa cgcgggaaag ctatcctttt gcgttcagcg ttaccatccg
     9661 gtatgtgctc aggggcggcg agctgcgctt tgagcagacc tatgagaata ccggcagcac
     9721 ggacatgccg ttctccttcg gtttccaccc ctatttccgt gtttccgatg tgcgcaacct
     9781 ggagtgggat atcaaagccg gcctgacggc cgacccggac acgggcagag acacgccgtt
     9841 tgaaggcgtg gatttcccct atgacgacga gcagaccacc cgctattata agggcgtgca
     9901 gagccccatg cgctttacag acaaagagct gggccacacc gtcaccgtga agtttgacgg
     9961 gaactttaaa aacgccgtgc tgtggagcca gtgcccgctg ggcttcgtgt gtatggagcc
    10021 gtggaacgga ttccccggca gccttaccac cccggaacac gagacgctgg ccccgggcaa
    10081 cgcactgagc gccgttatga gtattgaaat ttaaggcgat accgtttgca aagcagcagc
    10141 cgcatgccgg aagtgtattt cggcacgcgg ctgctgcttt atgcatcggc ggcccgctga
    10201 agaaaccgcg ccgccgccgt cgtttcgtca gcagaacacg aaagggagcg gaacggatgg
    10261 aacaaacggg aaaattttca aacgggcggg cggcgctggg gcttctggcg ctggtgattc
    10321 tggcggcggc gcagctgggc gcgcagcttc tggcaagctt gccgctgatg gtgggcgcac
    10381 cggtgtggct gggcaatgta ctggccggcg tgctgtatgc ggcgctgggg gtggcggggc
    10441 ttttgctgct gtgcggaaag ggcctgcgcg tgacgccgga ggaatgccgt cttgcaccag
    10501 tgcggctgcg ccctgtctgg gcggcgagcg cggtgctgat gcccgcgctg gcggcgggcg
    10561 cgctgctgtg tcaaccggga cattggcagg ctgggtctgc ggaggatgcc gcggcggtgg
    10621 cggcgggcgc ggtatttttc ttcggcgcgg gcacgggcat cgtcgaggag gccgtgttcc
    10681 gcggcgttct gatgacggcc gttgagcgcc gctggaacaa aacggctgcg gtgctcggcc
    10741 cctccctggc gtttgccctg ctgcacgtcc tggggcggga gctgagcttt gcgggcatac
    10801 tgcagcttct tgcggcaggc actgcggtgg gcgtgctgtt ctcgctggtg gcttatgaga
    10861 gcggcacagt gtggtgcggc gcagccatgc acgctgtatg gaatatggta atgatcggcg
    10921 gcgtgctgca catcggcccg gagcccgacg gatatgcgct gctcaattat gtgctggact
    10981 ccgcttctcc gctggtaacg ggcggtgatt tcggcgtgga agcgtctctt ccctccattg
    11041 cggtctatct gggcttcagc gcgctggcgc tggtgcggct gaagcggagc aaggcgtttg
    11101 cacacactgc gtgaacagcg cccggcccgg gcttgccctt cagccgggga gtggctgcgg
    11161 gccaagtggc aaaaaaacaa ccgtcccggc acacactgcc gggacggctc ttggctattt
    11221 gtccggagcg aaggaaaacc cccgccgctc cgcgggaatg cggcgcgcct ttattcggcc
    11281 tgcccggcca gcctggccag gtccaggcct gtcacatcgc tgacaggcac gctgaacgcg
    11341 atgccgcgtt cgcccgtacg ggcgaacgcc tcgttgcaga tggccttcat gatggactgc
    11401 ttttgggcag cggccgtcag gataagcact acgtcttttt ccggctggat ggtgatgtgc
    11461 aggaattttt cggcttcctc gctgctcagg ccgcgcgcac gcgccacagt gccgccgcgt
    11521 gcgcccgccg tcttggccgc ggccatcacg tcgtccgcca tgccgtgctc caccacggag
    11581 atgataagct cgaactggtt gttttccgtc atcgggggaa cctccctttc cacgttgatc
    11641 tcggcgcgca cccggccgcc gtctgtttgg cggtttgccg ccgcgctgat gccggaaagc
    11701 ggtacggtga atgcaatccc cttacccggc cgggaaaagc caagcgccag cgtcaggtcc
    11761 gccagcagcg gcgccaccag agcacccggc acaaggctga gcacaatgtc cttctccggc
    11821 tcgtccagcc caaggtaatc catcacctcg gcgctggcgg tgccgtgccc cagcatcacc
    11881 agctgcacgg ccacgtgccg tgcccggcaa aggtccacta cgcggtcgcc cttgccgcgg
    11941 tccacgatgg tcaccatgca gcgcacgggt gaaatgaaat ctgtcatgtt gcgtcctccc
    12001 cggccgatac gccgaattcg atgatctcct cgtcctgcac cgcgtgcact tcggctgctg
    12061 ccggcgcacg gcgcgttttg aactggtaca caaggccgat gatctggatg gtgatgagcg
    12121 gcgtcatggc gaccatggcc acgatgccga aggcgtctgt cagaatattg ccccccacgg
    12181 cctcgcacgc gcccatggca aagggcagca gaaacgtggc cgtcatagga ccagaggcca
    12241 cgccgccgga atcgaaggca atggctgtaa agatcttagg tacaagaaag ctgaggccga
    12301 gcgcaagcgc atagcccggc accaggaacc acagcagaga aacgcccgtc agcacgcgta
    12361 ccatcgaaag ccccaaagat accgacatgc cgatggaaag ccctgccatc atcaccttct
    12421 gcgagatggc gccgccggtg atctcctcca cctgcttgtt cagcaccagc acagccggct
    12481 cagccttgac gatataatag ccgatgagca tgcccagcgg cacaagcacc cagttataag
    12541 gcagctcggc cagctgcttt ccgatgtagt tgcccgcggg cataaagccc acgttcacgc
    12601 ccgtcaggaa cagcgtcagc cccaccagcg tgtacagcat gcccaccagg atctttacca
    12661 gagccttgcg gcgcagcttc aggaagaaga cctgaaacac cacgaaaaac gcgaggatgg
    12721 gcgccagcgc cagcaggacc tctttcgcgt aatccggcag ggcgtggccg aattccagcc
    12781 aaagctcccg cgtgctggca atgtccggaa tggacagcgg cgtatagccg gccccttcgg
    12841 ggttgtagat catacccagg atgagcacgg aaaggatggg cccgatgctg cacagcgcca
    12901 caaggccgaa gctgtcgctc tcggcgtttt tgtcgccgcg cacggctgtc aggcccagac
    12961 ccagcgccat gatgaacggc actgtaatgg ggcccgtcgt cacgccgccg gaatcgaatg
    13021 ccacggccag gaacgcgtcg ggcacgaacg ccgccagagc gaataccaca atgtagaagc
    13081 cgatgagcag ccacgaaagg ttccatccga acaggatgcg caggaacgac accaccagga
    13141 agaaccccac gccggcggcc accgtgaaga tgagcacgcg gtccggcacc gcgggcgtct
    13201 ggcgggccag tacctgcagg tccggctcgg ccacggtgat gatcacgccg ataacaaagc
    13261 aggctgcaac gatgagccac agcctttttg agcgcgtgag ctgtgtgccc acctgctcgc
    13321 cgatgggcat catggccatg tccgcaccca gcgtgaaaaa gcccatgccc agaatgagca
    13381 gcgcagcccc ggccaggaac agcatcagcg tggaaagctc catgggcacc accgtcatgc
    13441 ccagcgccag tacgatcagg gtgatcggga tgaccgcgct cagcgattcg tggatcttga
    13501 gtttcagttt cttgttcata tctcgccacc tttccatgcg tccgggaata ataccatatg
    13561 tattaatatt atcataagga aagggcgaaa gatgttaaga aagtataaag ataatgaaac
    13621 cggtaggaat tcccctttgt tcgcgggagc gaaaacataa aatcccccgc agcaggcgtc
    13681 gtcctgcatt caggggattt tatccgttct atgtttttta ccgcatctgg tcaaaactgc
    13741 ggcgggccgg atgtgagggc ggacccttat acctcgtaat cacagcacac gcgttcgcag
    13801 atgacgctgt gcacgaactc cttgaccttg cagtgggccg gatggtccgc gtatacggcc
    13861 agcgccgcgc ggtcctccag ctcagtgtac agcgccacgt cgtagccgtt gaagcccacg
    13921 cctacctcgg cgcgcagcag cccgggcaca acgcccacca gtgcctccag cttttgcttc
    13981 atgatggcac cgttctccgc caccttgtcc ttgtcggtca ggttccacat tacgatatgt
    14041 ttaaccactt ttcatttctc cttttagtat aattattgca cgctgcccgt gcggcgtttg
    14101 ttttcaacag catatcacgg tttttcttca atttcaatgt cctccaggcc aatatcgttt
    14161 ttgatgacgt tgcccggggc gatggaggtc ttgccgtatt ttctgcggat ggcgtccagg
    14221 ctctgttcca gccgttcccg tttttcgttc cggggacgct ccggctccag cagtgagagc
    14281 tggtgtccct cgcgtccgtc cgtcaggttc agcgccgtaa ccgtgagcat gcggatgggc
    14341 gcgtccaggc tccagttggc ttttaccagc tccagggccg ccccgccgat ctctcgcgcc
    14401 agatgggagg gccagggcag cggcttctgg cgggagatgc ttttcaggtc cgggtttttg
    14461 atgagcacct gcacggcagc agcgtaaagg ccgtgggcgc gcaggcgggt ggccacttcg
    14521 tccgccagcg cataaagccc ggcgcgcacg tcggcttcgc ccaccaggtt gcgcttgaac
    14581 gtcaggccgt ttcccacgct ttttatctcc ggacgctcgt ccgcggtacg cacgggagaa
    14641 ttctcctccc ctctggcgta ggcgcccagc tccggcccca gcttgcccag cgccgcgcgc
    14701 aggtcctcgt ccttcgccgc ggccagctgc ccgatgttcc ggacgcccag ccctgccagt
    14761 gtgttttgtg ccgagcgccc cacgtaaagc agcgtgttta cgggcagcgg ccacacgata
    14821 cgccggaaat tttccgtgtc gatgaccgtc acagcgtccg gctttttata atccgacccc
    14881 agcttggcaa ataccttgtt gaagctgacg cccaccgaga tggtgaggcc ggtctcggcc
    14941 ttcacggccc ggcggacggc gtcggccact ccggcgggag agctgccgaa cagccgccag
    15001 gtctgcgtca tgtccagcca gctttcgtcg attccgaacg gctctatacg gtctgtataa
    15061 cgggcataga tggcgttgac ggcacggctc atctcccggt actgcccgtg gtgcgggggc
    15121 agcagcttca ggccggggca cttgcgcagc gcgctccaga cggtttcggc cgtctggacg
    15181 ccgtaccgct ttgcgggctc gtttttcgcc aggatgatgc catggcggtc gtccgggctg
    15241 ccgcatacgg ccacgggcac gccgcgcagc tccgggtagc gcagcagctc taccgaggca
    15301 taaaagctgt tgcagtcgca gtgaaagatc acgcgctgtt cttccggcaa atccgccgcc
    15361 ctcctctcag gaacagtact ggacgattca cagctggcgc cttcagcacg cagctgtccg
    15421 tttgcgcgcc cgccttgcgc aggcgcaggg ccgtgcgtcc ggccaaaggc acgggcggcg
    15481 tctttgcggc tgtgccgccc cgcccccgct tgtaaatttt ccgctctgct tcacaaaaat
    15541 ccccgtcaat acacaaaccg cggcggccgg agccggacag cctgcgccca agacgcggac
    15601 tgcggttttt gtttcgtgcg gcgcaaaatt ttcctgtgcg gtcccgcgct ccgaattgtg
    15661 cggcgccggc ccaggttcct tttgcttatt ataacatttt gtagtataat acaaaataca
    15721 taacggcgca tcccgcgccg tggaaaaaac aggtaaaagc cgcatttgaa aggagctttt
    15781 gacatggaca gcaatgtgaa taaaattctg cagaatatcg tgaacaccgc caccctggcc
    15841 gccgacgagg caaaggccgc ggtgcacagc gccggaaagg ccgttgccgg aaaatatgat
    15901 gaagtgaaga tgaacatcga gcttgcccgc ctgtccgacg agcaggagga cgtattcagc
    15961 gacattggcc gcatgctgtt cctcatgcat acgggtgccg ttaaagatac ggtaatgagc
    16021 gacgagggcg aaaagtcccc gcagcaggtc atcgacgcgc tgctggtctc ggcggagcag
    16081 gtacagcagg agatggacgt catcaccgac aagctcaccg tgtgccgcaa cgagaaagtc
    16141 tgcccgtgct gcggccgcat ctgtggtgag cacgacgcgt tctgcgcggt gtgcggcacg
    16201 cgcctgtccg aggaagagcc gaaggaggag aaagcgcccg cggcagagcc ggaaatacag
    16261 cccgcagccg ccgacgcccc cgcggcggag ccggaagcag aagcggagct gcccgcggaa
    16321 taagcgcggc ggagaaaaca aatgacgtat acgatctacg aattgctgtg cttttttgtc
    16381 atctactcgg tgctgggctg gtgccttgag gtatgcttct gtaccatcaa tacgggccaa
    16441 ttcgtcaacc gcggcttttt gaacgggccg gtgtgcccca tttacggctt cggcatggtg
    16501 atcgtgctgg tggctctgac accgctggcc cacagccttc cggtgctttt cgtgggcggc
    16561 gcgctgctca ccagcgcgct ggagcttgcg gccggctgga tattgaaaaa ggtgttccac
    16621 accagctggt gggattacag cgacgtcccg ttcaacctgg gagggtatat ctgcctgaag
    16681 ttcagcctgg cctggggcgt ggccgtggtg gccgttatga aaggcgtcca gcccgcggtg
    16741 gcggcactgg tgcgcgccgt gccccgcacg ctgggcattg tgctgctgtg tgcggcaggc
    16801 gcagcctttg cgtgcgacct ggcggtgacg gtggccggca tcctcaagct gaaccgcgac
    16861 cttgggcata ttgccgaggt ggcgcgcctg ctgcacgcag gcagcgaccg attggccgag
    16921 ggcctgggcg acacggcgct ggccgtggac ggcaagctgg atgcaacaaa gctggacgcc
    16981 gccgcccgca tcgacatcgc caaagcggag atcctggacc accgcaacgc cgtggcccgc
    17041 cgccttttga aggcgttccc caacatgcgc ccggccgagc atgaagcgct ggagcagctg
    17101 aaaagctggg tcgaggaaaa aaatgccgct ctgcgcgcgc aggcggaaaa ggtaaccggc
    17161 aaaaagaagt gaagaaggag ctttatccca catgaaaacc accccgttga aaggcatgaa
    17221 agacatcctg ccgcgcgagc agcgcatccg cgactatgtg cagggcgaga tcttaaagac
    17281 ctaccgcgcc gcgggcttcg aacgcatctc cacgcccatt atggaagaca tggagaacct
    17341 ggacaaaagc gacggcggcg acaacctgaa cctgatcttc aaggtgctca agcgcggcga
    17401 caannnnnnn nnnatctaac actgccgctc tcgcgatact acgcggccaa ccgggagcag
    17461 ctccccgcgc cgttcaaggt catccagaca gaccgggtgt accgtgccga gcgcccgcag
    17521 aagggccgcc tgcgtgaatt cgtgcagtgc gacatcgaca tcctgggcga tgaaagcccc
    17581 aatgccgagg tggagctgat tgatgtgacg gcccgcgcgc tgctggccat cggcttttcc
    17641 gatttcaccg tgcatatcaa cgaccgccgc ctgctgcgcg gcatgctcga aagcttcggc
    17701 ttcgcgccgc aggcgctgga caccgtgtgc gtcacgttcg acaagctgga taaggtcggc
    17761 ccgcagggcg tggcggcgga gcttgaggaa aaggcgatgc ccgccgcggc ggtgcaggcg
    17821 ctggaggcct ttttggaaaa aggcgcattc tcgctggatg atgtatccgc gctgtgcgcg
    17881 gacaagagcc tgggcggctc ggtgcgttat gtgttggata ccgtgggcag gctgtccggc
    17941 ggccggtatg ccgtcgagta tacgccttcg ctggtgcgcg ggcagggcta ttacacgggg
    18001 atggtgttcg aagtaacgtg cgccgcgttt tccggcgccg tcgcgggcgg cggacgctac
    18061 gacgacatgg tgggtaagtt cctcgggcaa aaggtgcccg cagtgggctt ttccatcggg
    18121 tttgagcgca tctgctccat cctgctggac caaggctacg cggtgccgga cgaaaagccg
    18181 cgccttgcgc tgctgtacgg tgcggacgcg gatttcgcgg acgtgctgca aaaggcagag
    18241 gcgctgcgcg gagaatacgc ggtgacggtg ctggccgcgg ccaaaaaagt gggcaaacag
    18301 ctggaccgtt tgcagcagag cggctgcgcg gctgcgtgct tttatgaaaa atatcccgag
    18361 ataaagccgc tcggggaaaa cgcgtgagcg ctgcggccgg ggcgggcgac tcccaatacc
    18421 ggaaaatgac gcagacgccc atcccaaggc tcgttgctac gctggccgtg cccaccatca
    18481 tcagcatgct ggtaacggcg gtgtacaata tggccgacac gttttttgtg gcgcagctgg
    18541 gcaccagcgc ggcgggcgcc gtgggcatcg tgttttcgct gatggccgtc atccaggcca
    18601 tcggcttcat gctgggcatg ggcgcgggca atcttgtttc ccgctatctg ggtgcgaagg
    18661 agcagcggca ggcggactgt gcggcgtcca ccgcattttt tacggcgttg gccttcggcc
    18721 ttggcatcac ggtgctgggc acgctgtttc tggacccgct catgcgtgtg ctgggtgcga
    18781 cgcccaccat tctgccctat gcgcgggatt acgcgcgcta catcctgttc ggcgcgccgg
    18841 tcatgtgcgc gtccttcgta ctcaacaaca tcctgcgggg cgagggcaag gccatgctgg
    18901 ccatggtggg catcggcctg ggcggcgtgc tgaacatagg gctggacccg ctgttcatct
    18961 atgcgttcgg gcttggcatc gcgggcgcgg ccattgccac gctgctcagc cagtgtgtca
    19021 gcttcgccat tcttctagcg tgctttctgc gccgcaaaag cgcggtgcgc ctgcacatcg
    19081 ggcaggtgtc ccgcaaggcg gaggtgtacg cgcgcatcat caaaacgggc atgccgtcct
    19141 tctgccgcca gtctctggcc agtgtggcca cggtgctgct gaacgtgaac gccgccgttt
    19201 acggcgacgc ggccgtcgcg gccatgagcg tggtgggacg cattttcatg ttcgtgttct
    19261 cgttcatgct gggattcggg cagggcttcc agcccgttgc gggctacaat ttcggtgcaa
    19321 agcggtacga ccgggtgcgc ggggccactt atttcacaat gctggtgggc acggtgctga
    19381 tgagcgtgct ggcggcggcc ggttttctgg ccgcgccggg cgcgttggcg ctgttccgcc
    19441 gggacgacgc ggaggtcatc gccatcggcg cgctggcgct gcgggcgcag tgcgtggttc
    19501 tgccgctgtt cggcgtttct acggtgacga acatgctgct gcaggtgacg gggcagtccg
    19561 ggcaggcgac gttcctgtcg ctgtgccgcc agggcatttt ctttgtgccg ttcattctgc
    19621 tgctgccgca gctcataggg cttttgggcg tgcagctcgc ccagcccgca gccgacctgt
    19681 gtaccttcgc ggtgaccctt ccgtttttgc tttcatttct gcggcggctg aagctgtggg
    19741 aacaggcgga acgtgctgtg gcggaaaaat aaggatatgg aaaagccgcg gggatagccc
    19801 gcggctttgt tctttcctat tgcttcagtt tccgcgattc tttcgccaaa cgcattttga
    19861 cggtttccat ttttgaaata taccggctgg cgtataaaaa cgtgccggcc acaaatcccc
    19921 gtgcaatgcc cacaatggtt ccatacggct ccgtttccgc aagttccgct acgtccatcg
    19981 ccgcaaaaaa agccatgctg gcaagtgcca taacaaacat ggcacacatt cttctcgaaa
    20041 aaactttccg ctcgatattg ctatagtctg caattttcaa catcaattcc tctgcgctgc
    20101 cgtctgtgcg cccgcctttt ctttctccat ccaatatctc gctgacttcg accttatagt
    20161 attttgcaag ctcgatcaac aggtcgaaat ccggcatggt cgtgccgttt tcccaacggg
    20221 aaatactccg gttggagacg ccccgcgtct cagcaagctc cgcctgcgtg atagcttttt
    20281 catttcgcag cgtttttaaa aaatgacctg tttttgttga ttcatagtcc ctgtctcctt
    20341 tcgctcccta agaatatcgt tttcacccga aaaagtacag gtcacaggcc gagagttgtg
    20401 ggacggcaaa tgcaaaacgg tggatctgtc cgcctacaga ttcgccgttt tcacatcatt
    20461 gtttaaaaaa ctcatgcggt aaattttatg gcggccctgc ttcatgcggt ataagcccac
    20521 aggtttttga tctgcggctc caaacggctg tactcccaca gggatgcgct gcgggcggga
    20581 ctgttggggt ctacgacgca cagcagcccg tcctgcgtgc cggtcagcac gataaaatgt
    20641 cccgccgtgg taaaatcgcc ggggcgcatg ctgcaaatga tgggctgccc tgcttccagc
    20701 gcgccgaaca cggcggcttc cgtgagtgaa agctcctgcg cgtacaggcc aaagtgctcg
    20761 catccgctac gcatcagctc ccagctggtg ccgtccgcgc cggcataccc ctggcgctgc
    20821 gcccatgcgg cgatggctgc gggtgtgaga gaagcgtccc ccgtcaggcc gcaggccacc
    20881 accgagagcg ctgtcggccc gcagcccgtg atggcgagga tactgcctcc gtaaggcgtg
    20941 cagccccagc gcgcgtccca ttgcagcagc gtggggaagc tgccgcgcac ggcctcctcc
    21001 accctggcgg gcggcgcggc gtctataagg ctgggatacg ccagcacaaa gccaagcgct
    21061 tcctcattgc gggccagcag tgcggccacg tcggcgggcc agcgggaggg ctcgtccagc
    21121 atgggctgca cctcggggtg cgccgcggcc agcgtctcga tggccaagcg cgtctgggcg
    21181 tcccattcgc cgcccaggct cacagatgcg gacgcgccgc ccgttcccgc gccgctgtaa
    21241 ggtgtggcgg aaaccagcgc gggccagacc cgagccagca gcaccacccc ggcgcacagg
    21301 cagagcacac cgcagcagcg cgcaaaaaag cgccgcgcgc agtgctttcg cgcccgtttg
    21361 cggcggcgtg cccgggcagg gcggcggggc gcgtcgtcat agtgcagata acatgcaaga
    21421 tcattttcaa aacgttcgtt cacgggaaac catcctctct gtgagcctat tatagagaac
    21481 aaatcttaaa ataggctcga gcagagctta caagtttctt aaggcaacac cgcacaattc
    21541 acggctgacg ccttaagcgt cgcctcgctt gcagattttc cggnnnnnnn nnnaatgaga
    21601 aaagccccgt gaacgcgggt tttccgctgt tcttcccggc gtctcgaagg tcggtcgccg
    21661 ggtcataacg cttttctgcc gcgcgtgttg aaccatacca cgctgcaaag caccagcacc
    21721 gccccggcaa gcgaaaagcc ggaaagccgt tcgccatagg caagcgccac ccatacaggc
    21781 gtgaggatgg gctcgatggc cgtcaccagg ctggccgtca tgggcggcgt ggtggcgatg
    21841 cccctggcga tgaggacata ggaaagccca agctggaaca cacccagcgc aaagatacat
    21901 accatgggga caaagctgaa atccgtctcg cccgaaagga acggaaggcc gatcagcgcg
    21961 cccgccacct gtcccagaaa ataagacgac atcgcgtcgc cttccggctc ggtgtttacc
    22021 aggaatactc cggcataagc cacgccgctg cccagtccca gcgcgtttcc cagcagcgtg
    22081 cccgccgcaa ggccgtccgc cacaaagcag ccaatgcctg caaataccag cacgcacgcg
    22141 gccacggcgg cgcgcggcgg cttctgcccc ttggccagcc acaggtacaa aagcacaaaa
    22201 ataggcgcgg tgtacatcag caaaatagcg ttggccgcgc ccgccagctt ggtggcgaat
    22261 acatacaggt tcgttgtcag cgcaaggctc agcgcgccgc aggccacggc tccgttccag
    22321 cgcaggcgcc gcccggcccg ccacaaaaac gcgcccgtga tgcacgccgc aatggcgttg
    22381 cgcgcgccgt tgatggccag cggcgtccac ggcacaccct tgatgcacac gccgccaatg
    22441 ctgcacagca tcgccgccag cgccagcagc cacgcgccgt ttttctgttt catgccgctt
    22501 ccccctttgc aaaaatgcgc cgcttcactc ttgcaaagcg ggcgtttgtg ccgtatcata
    22561 aaaagataac aggcgccgcg cgcggcggcc cattcaaggg tatgaagcag gggttgtctg
    22621 atatacggag gggacgaaaa tgaaaccatt tatcgcagcg gatattttgc tgcccgcacc
    22681 gcaaacggat atgggcctgt ggcccgcgct ggcgtgcgac cagttcacaa gccagcccga
    22741 atactggcag aaggccgagg cgctcactca aaacgcgccc agcacattgc acatcacctt
    22801 gccggaggca tatctggaaa gcccggatgt ggacggtcgc attgcggcca tccacaccgc
    22861 catggccgat taccgcgccc gggtgctcac acgcggtgtc cacggttttg tatatgtgga
    22921 acgcgcgacg caaagcggtg tgcggcaggg ccttgtgggc gctgtggacc tggaggctta
    22981 cagctatgaa aaaggctccg caccgctggt gcgtcccagc gaaaatacca ttgtggagcg
    23041 tattccgccg cgccttgccg tgcgccgggg cgcgccgctg gaaacgcctc atatcatgat
    23101 gctgctggat gacgctgcct gcggcgtggt ggagccgttc gcgaaaaaaa aggcggcgct
    23161 ggaaaaactg tacgacacgg agctgatgct gggcggcggg cacattgcag gctgggcggt
    23221 aacggacgct gcggacattg cggctgtgga aaacgccgta gcggcgctgg gcacacaggc
    23281 ggcgttcgac gcaaaatacc cggacgcggc cggccggccg ccgctggccg tcgccgtggg
    23341 cgacgggaac cattcccttg ccacggccaa ggcgcactgg gaggaaataa aggcggccct
    23401 gccgcctgcc gcgcggcaaa gccatcccgc ccgctggtgt ctggtggagc tggtgaatgt
    23461 ccacagcccg gcgctaggca tcgagcccat ccaccgcgcg gtattcggcg tttccatgac
    23521 ggagctggca ggcgcgtttt tgtcgttcgc ggcgcggcac ggcgcgcatg cctgcggcat
    23581 ggccggggcg cagcagacct tttgctttgt ggacgaaacg tgtgcagggc cgggtacggc
    23641 ggagcgtgtg gagtgcctga aaaacgcgcc ctggcccctg gccgtgggta cgctggacgc
    23701 atttttgaca gaatttctgg cgcagcgccc gggcgcgaag gtggattaca tccacggcgc
    23761 ggacaatgtg cgcgcactgg cgcgggcagg cgcggtgggc gtcatcctgc cggatttcgc
    23821 caagagcgac ctgttccggg gcgttgtgct gggcggcgtg ctgcccaaga agacgttttc
    23881 catgggccac gccgaggaaa agcgatacta cctggaatgc cgccaaatcg cgccgcagac
    23941 attgtaaatt gcggccggac gcgctatact gttatagata aaggccgccg cgccgttgta
    24001 tcgcgtggcc tttgccgaat gccccgccag ttttgcttcg cgtggcgggc gccggggtct
    24061 gctcttgaat ttgtgccgac gcgaagcggc ggaatggatg atttttatgg aaacaatggg
    24121 aaacctgcgc cgtacccact attgcgggac gctgcgcgcc agggacgcgg gcacacagat
    24181 gacggtggcc ggctccatcg caaaatgccg cgacaagggc ggcgttattt ttgcggacct
    24241 gcgggacacc acaggcatcc tgcagcttat ttttgacgat tccacggacc gggccgtgtt
    24301 tgaaaaggcc tccggcctga aaagcgagta tgtcgttatt gccgcgggca cgctgcgtga
    24361 gcgcgaggcg aaaacggaca agatccctac gggcgaggtg gaactgttcg tcaccgagct
    24421 gcgcgtgctc agcggcgcgc agacaactcc ctttgaggtg cgcgacggca tcaatgtcaa
    24481 cgaccagctg cgcctgaaat accgctatct ggatctgcgc cgcccctcca tgcacgagcc
    24541 catcgtggtg cgctccaaaa ttgccaaggt ggtgcgggat tattacgacg agcagcattt
    24601 tgtggagatc gagactccta tgctcatcaa atccacgccc gagggcgcgc gggattatct
    24661 ggtgcccagc cgtgtgcagc cggggcattt ctacgcgctg ccccagtcgc cccagcttta
    24721 caagcagatt ttgatgctct ccggctttga ccggtattac cagatcgtcc gctgcttccg
    24781 cgacgaggac ctgcgcgccg accgtcagcc cgaattcacg cagatcgaca tggaaatgtc
    24841 gtttgttaca gaagacgatg tgatggccat gaacgaaggc cttgtaaagc gcgtgttccg
    24901 ggaagtgctg ggcgtggacg tggaaacgcc ttttcggcgc atgccgtaca gcgaggcaat
    24961 ggcccgctac ggctccgaca agccggacac ccgcttcggg ttggagctgc aggatgtgac
    25021 ggatgtgctg cgggaaagcg ggtttgcagt gtttaaatcc gccgttgagg cgggcggcag
    25081 cgtgcgcctc atcaacgcaa agggccttgc cggcgcgctc acccgcaagg agatcgataa
    25141 gctggtggac gtggccaaaa cctatggtgc caaaggcctt gcctacacgc gcctgacggc
    25201 ggacggcgtg acctccagct ttgaaaagtt cctctccgag gaggaaaagg ccgcgctgca
    25261 caaggcggcg ggcgcagaga ccggcgacgt gctgctggtg gtggccgatg cgaaaaacag
    25321 caccgtgttt gcggcgctgg gcgcgctgcg ccttgccgtg gcaaataagt gcggcctcat
    25381 cgacccggac aaattcaact tcctgtgggt gacggatttc ccgttctttg aatattctga
    25441 agaagagggc cgctggatgg ccatgcacca tccgttcaca atgccccggg ccgaggacct
    25501 ggacaaagta gagagcgacc cgggcagcgt gcgcgcgctg gcctacgaca tggtgctcaa
    25561 cggctgtgag ctgggcggcg gttccatccg catcaatgat ccggccgttc aggaccgcat
    25621 gctcaaggcg ctgggcttca gcgaggagcg cgcgcgggag agcttcggat ttttgatgga
    25681 cgcctaccag tacggcgcgc cgccccatgg cggtatggcc ttcggcttcg accgcatggt
    25741 gatgctgatg ctgaagcggg attccatccg cgacgttatc gcctttccaa aggtgcagaa
    25801 cgcgggcgag cctatgagcg gctgccccga gaccgtggag gaaaagcagc tgcgtgagct
    25861 ttccatcgct tatgcgccca tcgaaacgga agaataaaac caaaaggccg ctgcgggagt
    25921 tcnnnnnnnn nncccgcggc ggccttttgc cgtttgtatg taatttgaaa aggcatcaat
    25981 cctgcgggcc gagataagcc gagctgccga agccgaggat gagcataaaa atcggctcaa
    26041 agaaaagcat gccgatggca aagcccacgc catggccaaa tgcacggcac ttgcgcacga
    26101 caagcaggat gttgacaacg aacgcaaaca acagcagcag catcgcgatg aaaaacatgg
    26161 cataattaat cgagccgtcc gcctgaatgg agaagggaga cagcacgaaa gccagaacgg
    26221 tagccgccag agtggcccag aaaagattct ttttcttcca ggaaatttta taggtgatgt
    26281 aggtgctgta aatggggatg atggatttcc agccggcttc cccggctttg gtaaagatcc
    26341 gccatgcggc aaccacatgg agcagccacc acacaaaccc cacgatcgag tatgtggcca
    26401 gcatgctcag gaaaaacacc atcatgctgt tatccataga catacatgat tcctccactt
    26461 tttatattgg aaacggtaac cgtccgtttc taagaggatt ataataaaaa aagacaaaaa
    26521 atgcaattct ctgtattttt cacagagccg aaacaacgcg gcaaaaactt ttgacaagga
    26581 cacggaaatg cattaccttg cagaaaatac gaaagcggca gtgccgctgg aggaacagcc
    26641 gaatgcgcat gggagataac cgcccgcgcc gggacagacg ctggtactgt acgacggcgg
    26701 aacagcggtg ggcgggacgt ccttttttca ctgtctgaaa caaaaaacgc acagcgtacc
    26761 gccaccggca cgccgcacag ctcctcgagc gtcttgagtc ccggctccag gatcttctta
    26821 tccccgcgga atttgttgac gatggtgccg cggatgcgcg ctcgctcgtc cggctcgagc
    26881 agcgccacgg tgccgtagag ctgcgcgaac acgccgccgc ggtcaatgtc gccgacgagg
    26941 agcaccggcg cgtccacgag cttcgcaagg cccatgttca cgatgtcctc ctgcttgaga
    27001 ttgatctccg cagggcttcc cgcgccctcg atgacgatga tatcgttctc cgcggcgagc
    27061 gagttatacg cttccatcac cgcggggata tactcgcgct tgcggcggta atactccatc
    27121 gcgtgcatgt tgccctgcac gacgccgttg acgatcacct ggctgccgac gtcggtcgtc
    27181 ggcttgagaa gaatggggtt catgcgcacg tccggcgcga tgcccgcggc ctccgcctgc
    27241 acgacctgcg cgcgccccat ctcgccgccc gcggcggtga tgaaggagtt gagcgccatg
    27301 ttctggcttt tgaacggcgc gactctgtag ccgtcctgcc ggaaaatgcg gcacagtccg
    27361 gcgcagagga tgctcttgcc cgcgttggac atcgtgccct ggatcataat gtttttcgcc
    27421 atggtcagac ctcccgtagg gcgcggatga gcgcctcgtt ttccgcgcgc gtgcgcacgg
    27481 cgatgcggta ccagccgggg caaagccccg cgtaattttc gaagtttcgt at
//
LOCUS       DAWBSB010000005        25234 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_536_length_25234_cov_3.481075, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000005 DAWBSB010000000
VERSION     DAWBSB010000005.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 25234)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 25234)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..25234
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_536_length_25234_cov_3.481075"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    278..287
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1155..1164
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3707..3716
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7293..7302
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10581..10590
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4925 a         7148 c         8119 g         4992 t
ORIGIN      
        1 gccgccgtgc gtggcgacca gggccttggc actggtcgta agcagcttgt cgaaaaagct
       61 gtgcttttcc gacaagctga accggacagg agtaaaatct gtccggtttt tgttttattc
      121 cagcgtcacc acgcgctttt gcgtgtagga ttccagggct gccgtgagca cgcgatggct
      181 cagatgcgcc tcggccgggg tgaagcagcc gaaggagcag ggaaggccgc gcagctcgct
      241 gacgaacgcc gcgaacattt gcagcaggga atcggaannn nnnnnnncgg aaaagccgaa
      301 ttcaaaaata gagccggtga taacgggata cagcgttttc tggccgatga ccagccgcgc
      361 ccatgcctgt tcacggccga cggcctgtgt ataatggatg gcgttgggat cgtcggtggt
      421 gaaccgggcc gacaggttga gaccgtaaat ttcatactcc acggtgttgg tgcagccggg
      481 ggccatacgc ttcatttcaa gctgcatggg gaacgtatcc cccgctgtgt tttgcgcttc
      541 gcacatcagg atggcgttgt cccaggtttc acagggaacg ggcacgccgt tttcgtccgg
      601 ccgtgttttg gcgatattgg caagctgcgc gctgacggtg cggggcgaaa aaccaagccg
      661 gaagggcagg tgctgcgtgt ggatgcccag gtctcccata cagccgtatt ctccgttggc
      721 tgcacgcatg cgcttccagt tgatgggttt tgccagatcc atgtcggagg aatgcttgat
      781 ggcgaagcgt gcctcgatga tctgcccgaa tgcgccctcg cggtaccatt ttaccatctg
      841 ctgtacggcg ggatagaatg gaaattcgct ggcgcaccgc acaaatacct cggggcgacc
      901 ggccatggcg gcctccacct cctcacaggc cgcaaggtcc atgccgaaag gtttttcccc
      961 gatgaggtgt ttccccgcgc ggatgacgtc gccgtacacc tgtgcgtgca ggtggtgggg
     1021 caccgcgcag tatacggcct ccacctcggg attggccagc agctcgtggt aatcgctgta
     1081 aaagtatttt gtgtccggca cctggcggaa ccagtcgaga ttttttggcg agaggtcgca
     1141 cgcagcaaca atgannnnnn nnnnacaatg acggggcggg gcagctcgca tgccagatgc
     1201 agccagcgca tggagacgct ggcgaattcg cggcccatca ggccgcagcc gatcacaccg
     1261 aagcgcacgg gttccatatt catagggccg ctcccttctg cgcggctgtc tcggccagcg
     1321 cgctgtcaaa cagggcgacg agccttccgg ccatcgtctc tgtggtaatg agcggcagct
     1381 ttgtaagagc taccggcgga caattgggct tataggtctg tgcactgatg tccacgcagc
     1441 ggtcccggtt catgcgggta cagaaatcca ccgcatcgtc cacgctgtcg aagcacagcg
     1501 ccgtcatgtg ggcgtcacct tccacgcctg tgcatacctg cgggtatttt tgcgccagtg
     1561 cgcgtacctg ttcgtggtaa taccggcccg cggcttcgat gtgcgcgccg ttggccccga
     1621 tgaattccat tgtcacaaga taagccaggc tggccagttc ttcctgtccg ttggtcacaa
     1681 gcgcaccgaa ctggctcagg ctgtcgaaag cggcgctcgt caggatcttg ctggcggggt
     1741 aaacgccccc ggggaagccc ttgcctacgg agacaaagtc cggcgtaagg ccgtatttgc
     1801 ggaacaggaa cagtccgtca taccatgcac aggactggat ctcgtcgcac agtacgggcg
     1861 tgtcggtggc gcggcacact ttgtacgctg cctgcagata ttcctcggaa aggcggatgc
     1921 cgccgtaatt catgaggacg atctcgtgac agaagcccgc cgttttgtat ttgccctcgt
     1981 tccagcggcg catggtctcg gcaaaggcgc cggagtcgtt gatggggacg gacaccacct
     2041 tgtacagctc gtttttctgc gcggctgtgt agaagtccgg ccacaggccg cgcagcgtct
     2101 gcgccagcac cgtagtgccg tgatagtttc cggtcacgcc gccggcattg tctgccatga
     2161 caacgaacac gggtacgcgg cctgcatact gtgcgggcgc gccgtcgatg gtgtaaaagc
     2221 gggagagcat cattttgagc gcggcctcca cggccaggga gccggtctcc agattgatga
     2281 cggtattcag cacgcccggc tccttttggg acagaatgcg gtccagcgcc gggtctgtgg
     2341 gctggaggcc gttggcggcg gcgatgaggc ggcgctccag cgtgcgggtg ataaagccgc
     2401 gggtgttgtt gtgcgtagca ttggggattc cgagacgctt ggcgttttca ataagcgagt
     2461 agcctggaaa gtcgtgtccc aggggaatct ggtaatgctc gcttttgccc gcgaaataca
     2521 ggcggccgtc ctcgccgatg cggaatgcgc ccagcccgcc cagcggagct gcaaagctgt
     2581 gctgggcggc acggtatgaa tgggtgggcg cgccgtcctc agtgtctgca aaagcgggcg
     2641 caagggagtg gccgatgtcg cccatcagct cctcctggcg gcgggcaaaa gcctcgggat
     2701 aaaaatcgac cttctcatcg gccacggcgc gcagagcttc ggggctttcg cctgtgagtg
     2761 ccgcgcgcgc gcggcacaca gccgcggtgt attccgcgcc aagcaagtcg gtaagggaac
     2821 gccggatgtt ctggatcata tcaattctcc ttttctgcca ttgcggcgta ttgtttatac
     2881 ttcgacgaac tcaatgccca gcaggcggca cagcagacgg acgcgcgcag catagtcgcc
     2941 ggggacgatg atctcatggt gagtgccgcc ctgctccagc catgccttga tgcaggcgcg
     3001 tacgccggat gcgggccgga agaacatata cggcatgtcg atcttgcgca ggctgcattc
     3061 gtccagtatc tcgcccgggg catatacgag gcggaagcgt tcgccgccca catgcaccag
     3121 gctcatcacg cctgcggggc cgggttccgg cgtgaagatg ggtgtgggcg ggttggaaag
     3181 gccgccttca ttgaataccc ggtccatcag gaaaggcttt ttgtccctgc ggcacatggc
     3241 ccagttgccc tcgcccgcgt ggcacagcag gatggcctcc cgcgcaaaat ccatcatata
     3301 catctcgctg aaaccggcct gcccgcacag gcgcaacata gccgctatga gcatggcggc
     3361 agtggcgtcg ccctcggctg catagccgta gccgtcggcc atcaggctgg aggcggccag
     3421 gaagggaaga tgctcgaacc gtccgtctgc tccgaattcc tcgaagtgaa cggtataccc
     3481 cgcatatccg ttttcctcca gataacgctt gaggcccaga tacatgcgca cggactccgc
     3541 atggcgttcg ggcggcagct tggggtcgat ctcaaaaaca gtgtgctcgt acgccacgcg
     3601 cgcggagata tcctccggcg tgaccccggc gcaggcgcgc tgcacggcgc cgatggaatc
     3661 gtatacgaat tccggcccca gcttgcggta aacggccata tcatcgnnnn nnnnnntgtg
     3721 cgccgccgcg ccgaaatccg ctgcaaaggc tgcaagctcg ccgtttttgc ggctgcccgc
     3781 gaagaatgcg caggggatgc cggcccgcat caggcagttg gcgttgtcct gtgagccgtg
     3841 gatgccctga ttcactgtga gctcccattc ctcgaaatcg tcgcccacgg tctcatccgg
     3901 ctgcaccagt gccagcgcca acggaagcgt attgcgctgc atggcgtgta ccaggtactg
     3961 cccctgagaa tagctgagca tcaggatgag gatgccgtcc agtccctcgg cgttgtattg
     4021 tgcggtgagg gcttctatgg cctcccggct ggcggcgatt cgcggaaaga caaaatcggc
     4081 tgtaccggag agtgaggcga tgacctcctc gacatatttg tgctggctgg ccatgatgcc
     4141 cggaaacaaa ggctcgtagc cctccagcat cagcgccagg atacccagac gcggcttgtg
     4201 ttgtaactcc atatcctttt cctccttttt gacaatggga aggtttttcg tgcggcaaac
     4261 gccgtgaatc tgtggacgat gccgggctcg cggcggcgta cggtacgtcg tattcgcggg
     4321 ccgcccgggc gcgcgtcgct ttcggcaagg tgccaaaagc ggcgcgcggt gcggtttgtt
     4381 cacgggcagc ccctttaaaa cgcgttgtac agcggtttga cggcggcata aagctttttg
     4441 tacaggctgt atgccgtgtc ataagcgggc gcgcgggcgt tgtctggaag atagacggcg
     4501 ctttcaccgc ggcaagggaa aacgccgggg gcgatgagtc cgctgcccca cccggccaaa
     4561 agggcggcac ccatggcggt ggcctcgctg cacgcgggca cgcggaacgt gcgcccgcag
     4621 gcatcggctt tgatctgctg ccacacagcg ctgcgtgcgc cgccgcccag cgagtacacg
     4681 gtgtcggcgg gacaacccag agcgttcagc atgccgaaaa agtcccgcag cagaaacgcg
     4741 acggattcca tcacgctgcg ggcgaaatgg gcccgcgtgg tggaaagccg cgcgccgaag
     4801 aatacggcgc gggcttcggg acaggcgtcg ggatccacgc tgccggataa aaacggcagg
     4861 aacgccagcc cgtcgcaccc gcaggggacg ctttgcacca gcgcgtccag cgctgcatat
     4921 cccgctgctc ctgcgggaag gtcgcggcag aattcgtccc ggaaccagcg caacgccatg
     4981 cccgccgtgt tgccgatggg caggtacagg tacttgcccg gcaatgcgtg gcggtagacg
     5041 gttacgtggt gcccgctgcg aaacatggga gcgtctgtac aggcggccat taccagcgcg
     5101 gtacccgtgg tttcggtgac ggtgcccggg cgcgtgcagc ctgcggcaag cgcggcggcg
     5161 gtctggtcca ttgcgcccgc aacgacgggg atgttcggtg gcaggcccag ctcggcggca
     5221 gggccgggca gcagagagcc tgcgggcgcg ccgcattcca gcagttcggg cagcagttcc
     5281 gcagacgcgc cggcggcggc aaggccgctg tgccacaacc cgtcgctgtg caggtcgaac
     5341 cagccggtgg aggtctgcag ggatttttca cttacgaacc ggccggtcag ccaatggagc
     5401 agatagtcct ccagcagcag aagcttatgc gcgcggacaa aggtatccgg ttcgtgtttg
     5461 cgcagccaaa gcagctttgc cagcggaagc gcgccggtga cctccggcag gccggtggtt
     5521 ttgtaaaatg tctcgttgtc cagcacactg cgcagagcct gggcctcatt ttccgcgcgg
     5581 ctgtccagcc acaccagaaa ggggcgaagc ggctcgcccg aacggtccgc gagcgtgagc
     5641 gtttcgcctt gtgtggtgat gcttacggct gcgggaatac agccgggcgc ctgggacagc
     5701 gcgtcggcaa gcccggcccg cgcggcggct ttgtactgcg ccgcgtcggc ctccacgcgt
     5761 tcgccctgtg tgcgcagggc atattcctgc acgctgcacg ccagaagttc cagcgagggg
     5821 gagaaaaggc aagctttcac ggcggtggtg cccacgtcca gcaccgcgat acagcggctg
     5881 acggcttcgt gtgcctgtat ctgtgtgccg cccatctctt acgcctttcc gtcgctgccg
     5941 aatacgcgca tatgatgcat cgtttcgtgc ttgacggcct cggtgatgaa cggcatcagt
     6001 tcaaatgcac caacagattc cgtgaaatgg gtgtgagcgg cacgggcggc tgtcaggtta
     6061 ttgtgagtga aaatgttgat tttggagatg cctcccgcaa cacaggcgcg gaaatcgtcg
     6121 tccgaaagcc cgctgccccc atgcagcacc agcggcgttg agcatgctcc cgcaatactg
     6181 gcgaggcagt ccagattaag cacgggcttt tccgcgtata cgccgtgggc cgtaccaatg
     6241 gcaacagcca gcgcgtcgca gccgctttcg gcagcaaagc gcagcgcgtc ctgcggctgg
     6301 gtataaatgg ttttggcgac ggcttcttcg gcggagccgt tggaaccgac atgaccgatc
     6361 tctgcttcca gtgagcagcc cgcggcgtgc gccttggcgg tcatttcacg ggtgcggcgc
     6421 acattttctt caaagggcag catgctgcag tcgtacataa cggaggaaaa gcccaggcgg
     6481 atggcttctt ccaccagctc cggcgtgaag ccgtggtcaa agtgcagcgc aacgggcaca
     6541 ccggcgcggc gggccatgcc cagcatcatg tcggcatatt ccgcaagcga agcgccgggc
     6601 aaaaggcttt cggcgggccc gatgatgacg ggggagtgca attcctccgc tgccgcaagg
     6661 atgcctttcg tcatttccag tgtgacgcag ttgaacagcc caacggcata gtgcttttca
     6721 cgggtgtccg cgagaatggt tttcaaatcg actaacatag cagtcgtcct ccttttttaa
     6781 caatgggaaa cttggccgga tgccggccgc ctcgaatctc cctgcgcggc cggtataaca
     6841 aagagctttt gacaatgaag aatccggccc gatgccgtcc gtggctctcc ctgcgcggca
     6901 gacgtgccgg cccgggcggg atgctgatga aaaagctttc ctgtgcggag cttttgcagg
     6961 tgaaagggga tggatatttg cgcggtccgc gtcaatactc gatggaaagg aagcgcccct
     7021 ctgcgtccgc ctgccgggcg gcgaggcagc ttgcggcgct cacaagcccg gctgaaaggt
     7081 cggagcgtgc ttctccgccg tccagcacgc gcagaaaatc cagtgccagc atctcatctc
     7141 cgccgaaatg catgcctgcg ggaaagttga atttgtgggt gactgtctgg ggggataagt
     7201 aacggtccac acggatctcg gcggtgtaaa aatcaaattc cgcgctgccc tcggtaccga
     7261 taagccgtgc gccgcgccgc ccggcgctct ttnnnnnnnn nngttgtagc tgatcagcat
     7321 gccgctttcc gttgtgaaaa tgacgctggc gccgtcctcg ttgccggtgt ctgaggcaaa
     7381 gcagcaggcg tcgccctgga catcttcctt caaaatgttt tttaccacgt agctgctttc
     7441 ggggcaggtg cggtaatccg ggcaatccgg acagtgcagg cccgccggac ggtccccttt
     7501 gaaatgcagc ttggcggtct tagcgcacac ggaaacgggg cgctcgcctg tgaggaagtg
     7561 gatgtagtcg atatcatggg tggccttttg caggaacagg ccgccggtct cggccgggtc
     7621 gcggtaccag ctgtgataat aaacgctgcc gtaggggaca ttgttgacgg cctgcaccat
     7681 cgtaagcctg cccagcacgc cgctgtccac aatgcgcttc atctccaatg tgatggagga
     7741 aaggcgcagc gggaatgaaa cgaccacttg cttctccttg cctgcggccg cgcggcgcag
     7801 ctgctcatac tgggggcgtg tgatgcacac gggcttttcc aaaaacaggg gcaatcccat
     7861 atccagcacc cggcaggcgt agggggtgtg cagcgagcag cgtgtgccga tgaacacccc
     7921 gtccagcgct tccccggcaa gcagctcgtc cgcgtctgca taccagcggg tatgggcgaa
     7981 ataagggtcg cctgccagac cgggggcgat ctcggcatgg cgcgggtctg ctacggcggc
     8041 tacggtgatg tctgcctcca gcgaacggaa ggatttcatc attgtttctg agcgcagacc
     8101 atatccgatg aacccgatgc gtttcataat tccattcctc cattttttgt gcggggccgc
     8161 ttttctttat attacatata aaaggcgggc aaatggcttt gcagtgcgcc tgcgccgctt
     8221 tttatacgct tgctgcgcta ataattattt tgttattagc aaattttaag agaaaatatt
     8281 gtagattttt actaaagaaa ggccctactt tttgaaaggt gatttgcttg acaaccccga
     8341 tgaatgctga tatagtttaa ttgaaatcaa ggattgcaag gttattgtgc cacaggtgac
     8401 atctaaaagt caagtaggtg aaagcaaata actttgcaca gaaaagacaa aattattaaa
     8461 aatttattac taataatttt gaatcatccg ggaggttttc aaatgtccga ggaatcaagg
     8521 gccatgcggg cccccaatgc gctgcggcgg ccaaacaaat ttattgcgta tatgcgcaaa
     8581 tactggtttg tttacatgct ggtgcttccg gggcttgtct tcatgcttgt gttcgattac
     8641 ggtcccatgt acggtattct gcttgcattc aaggattatt ccccccgctt gggcgtgtgg
     8701 ggcagcccgt gggcgggcct gtctcatttc cgggaaatgc tggtagaccc aaacttcatc
     8761 cgcgcattta aaaacacggt gctcatcaac ctgtataatc tggcgttcgg ctttacattc
     8821 aacgtgtttc tggcgctgat gatcaacgag ctgaagatca agaaggttaa acccgtggtg
     8881 cagacctgtg tgtatctgcc gtacttcctc tcatgggtca tctttgccgg cctggtgcag
     8941 gtgtttctgg agtatccgtc ctcggccgat atcggcggag tggtgaacca ggtcatctct
     9001 gcattgggcg gccagccggt ggactttttg aaaaacccgg ccatgttccg caccatcctt
     9061 gtcatcacga acatcatcaa aacggcgggc tattccacca tcgtgtatct ggcggcgatc
     9121 tcgggcattg accctgcttt gtacgaaagc gccgccatcg acggcgccaa ccgtaaggat
     9181 atgctgctgc acatcaccat tccgcgcatc cttcccagcg tgatgatcat gctgatcctg
     9241 caattggcga gcctgttcat ctccaacttt gaccaggtat acaacctgta caacaactat
     9301 gtgctcagca ccggcgacgt gctttccaca tatatctacc gcatcagcct gggcggctcc
     9361 ggcacgaatt tcgagctttc cacagccatg aaccttgtgc tcaataccat gggccttatc
     9421 gtggtgctgg gtgcgaacaa atttgtggaa aagctggatg tccagggcat attctaagcc
     9481 ggaaggaggg ccttatcgat gcgttcaaac aacagcttgc gcagaaacgg caaatatcgc
     9541 cgcagcatgc gccagatact gggatttaac ttctacgatg tattcgtatt tcttttctgc
     9601 atcgtctttg cgctggtatg cgtgtatccg atgtggtatg ttctggtggc gtccgtcacg
     9661 ccgtatgagg agtatatcaa gggcgggctg atgctgtggc ccaccggcgg tgtggacctg
     9721 cagtattaca aagcgatctt cagtaccaaa agctttacca acagcatgtg gatctcggtg
     9781 agcaagacgg tcatcgccac tgtgctcagc gtgctcgtca cggccagcat ggcttacggc
     9841 gtcagcaagg tgcacgtaaa gggcatgaag gtcatcaacg cgctggttgt gttcaacctg
     9901 ttctttacgg gcggcctgat ccctcagtat atcctctatc agaacctggg gcttctgcgc
     9961 aattactggg tcatggtcat tcccggtgcg ctaaatatca cttatttcat catcatgcgc
    10021 aactattatt cttattctgt gccaaagtct ttggaggaag cggcactgct ggacggctgt
    10081 acggatatcg gcgtgttctt ccgcatcgtc atccctgtga gccgcagcat gctggcggcc
    10141 attgcgctgt tcatcgcagt catcaactgg aacgattatt acaactacat gatgtttatt
    10201 tccaataaga cgaatctgca gcctttcgcc tggattctgc gccgcatgct ggtggaccag
    10261 accatgatga accaggtgcg caacggggcc gtgggcatgg gcctgcagct tccgccgccc
    10321 atggcgctgc gcatggcgac cattatctgc gccatggtgc ccatcatgtg cgtgtatccg
    10381 ttcctgcaga aatattttac cagcggcatc acgctgggag ccgttaagga atgatgttat
    10441 tgccccacgc tgcggggtag aacatataga accataatca acaaggagga ttaaccatga
    10501 aaaagcattt acacgttttg gcactgcttc tggtggccgt gatgcttgtg tccgcgctgg
    10561 cgggctgcgg cggctcaggc nnnnnnnnnn cctgccgacg ctaaccccaa cgccggccgc
    10621 gagacggtca acatccgttt cgctcagttc ggcaacagcg tcgacgacgt ggacggcatg
    10681 gagaacgacc ctattaaaaa agcgattgaa gaagccgtga acgtcacgct ggagtacgat
    10741 accggcacag atgggtttga cgagcgcatg cagaccgagc tgttcacggg cggcgccgcc
    10801 gacctgttcc ccacctgggg cgagagcgag aagatcagca aatgggctga agaggacctc
    10861 gtcgttaata tcgccgagat cgtcaatgct gagcccgacc gataccccac gctgtacaaa
    10921 atcatcaaca gcgacgagta caaggcttac aacaagctgt acacaggcga tgaaaacgcc
    10981 gcctatgcca tttattccgt ggccgcgttt gcggacccca gctttgccgg tgtgcccgtg
    11041 tacaatactg cgattctgaa cgaagtgaac gagggaaaaa cgcccgctac cgtggaggaa
    11101 ttcattgatt tcaccaaggc gtgcggcgcg gccggttatg ttggctggtg gccccgcaac
    11161 gacaaactga ccaactggca gcagattgac aagactctgg ctgccccgca gggcacgagc
    11221 attctgcctc cgacgggcga cgtctggagc gggtttgtgc ccagcggcga aatcggtacc
    11281 gacagcgagc actggacgct ggccactgtc agcgatgaag caaaggaagt tgtgaaacag
    11341 cttgccgaaa tgtatgcggc gggcggcctg gattccggta tcggcgtcaa gggcgatttc
    11401 gatgacgcat actctgattt cggcgcgggc aagatcgctt ctgcggactt cggctttggt
    11461 tatccgggcc agttccgcga cttttataaa acagcctggg ccgctgccaa tgacggcgcg
    11521 cagatgagcg acctgacgct gggcacgtct ctcacgtccg acggaaatta tggtaaaaca
    11581 tataccacag gcacgtggat cggtgcgcat tactttattc ccactacctg cgaaaaccct
    11641 gaccgcgtgc tcgacctggt ggagtatctg gcaagcacag agggacagga cctgctgcac
    11701 aacaccacaa attacgagtt ccgcaacgat cagggctctg atttctggca gcccatcaac
    11761 aaagcttacg gttatgacga caaccgctgc aaatatgtct ggttcagcta cctgctcagc
    11821 ggcacggaat atgaagtgaa ctttgcagac aacgattggt ggacggccgt gtcccacccc
    11881 atcgacaaca gcaacgagtg ggccactgaa gaggacgccg cgcttgtgga ttatgccaag
    11941 gggatcgtct ccggcttcgt ggatgaagcc gtggagtatc tgccggcgta ctacaatctg
    12001 atcagcctgc cctctgaggc cgcagatatc cgcacgaagc tgcaggatat ctccaaccag
    12061 tatctgacgc agatgatcgg cggccagatg gacgtcgaga cggcatggcc gaattatgtg
    12121 gccgagtatg aggctgccgg tgcagccgag ccccggcatg acgggattcc ggtccaaaca
    12181 gaaaaatatg cgccgcgcgc cgcgcggcgc agaacaaaac gattcaggag ggtatgaata
    12241 tgccacaggc aaagctgtcc gatccttccg gccgcggcaa ggccgagggg tttaaggtga
    12301 tttttcccat gatgcagcac ccgtacacag aggccgagat ccaggctgtt gtagatgtga
    12361 tgcgcaatgc ggagggccag acgcagggca aatatcttga aaagttcgag aaggacttcg
    12421 ccgcattcgt gggtgcgaag tatgccttcg gcgtaaacaa ctgcaccaac gcgctgaagc
    12481 tggcggctat cttctgccac atccagcccg gcgacgaggt catcgtgccc gcgtatactt
    12541 actgtgcgtc ggccattccc ttcggcgatc ttggcgcaca tatcgtctgg gcagacatca
    12601 ataaggatac ctggaccatc gaccccgatg atttcgagcg taagatcacg cccaagacca
    12661 aggctgtggt ggccgttcac ctgctgggca tcccctgcga catgaagcgc atcgtggcca
    12721 tcgcccggcg tcacgggatc aaggtggtgg aggactgcgc acaggcgttg gactgccgca
    12781 tcgacggcca gcatgtgggg accttcggag attttggctg cttcagcttc catgcggcca
    12841 agacgatgac gactttgggc gagggcggca tgtttgtgtg cagcgacgac caggtggctc
    12901 acaacgtgcc gggcatccgt cacaacggcc tgtgcgcgtt ccagggggaa cgtgagcgct
    12961 actgggttcc cgccatgagc aacgtggacg aattcctgga ggaccgctgg ccgcagaact
    13021 tctgcctggg cgaagcgcaa tgtgcgcttg gctccgagca gctcaagagc gtgatcgcca
    13081 ataacgagat cctcatcgaa caggaccgca agatccgcga atggctcaag gatgtgccgg
    13141 agatcacttt cccgggcatc gttgagggag ggcgctatgt cgtccaccag tacatcatgc
    13201 actacgacgg aagcaaatac ggaaaaaacc gcaacgacct gctggacctg ctgacacaga
    13261 aatacggcgt acgcgccatc gtgcagtatt atccgctgta tcgctacccg ctgttccaga
    13321 aaaagggctg cggcgcgtac gactgcccgg tgctggacgc ctggtgggac aacagtttct
    13381 cgttcccgtg gtggtgcggt atcgacgacg agagcatgcg catcatgtgc gaaggcctca
    13441 tccatgcggt gaaggacctg cgcggttgag cgcgggcaaa aacggaacgg tatcctgaca
    13501 agcgaaaatg aacagggaaa cggccgaaaa ggcagcgggc ccgatggcgt cctcggcgga
    13561 gcatcggacg ccggcctttt cggtttcgtt ttgccattac ggcggagagg ataaagaata
    13621 tggcattcga caagacacgt ttcgggggcg tggtggctcc catcgtgaac ccctgcggtg
    13681 aggacgacag cctggaccgc ggcgcactgg aggcaaacct cacccggctg ctggcaacgg
    13741 atgttgcagg gctgtacatc aacggcggca cgggcgacgc ggccaatctg acgcaggacg
    13801 agcggctgga aacggcggcg cttctggtgc cgcgcctgct tgccgcgggc aagctggcca
    13861 ttgtgcatgt aggccagaca aaccagcgcc aggccgtgga gctggcgcgg caggccgctg
    13921 cgctgggcgc gcatgcggtg gcgagcatcc ccccgaaaaa accgtggccg cagatcgtag
    13981 aatattacaa ggcgctggcc gccgccggtg cgccggtgat cgtgtattat atccccggcg
    14041 tcacaggcat gacggcaggc atgccggagc tgcgcatgct gctggacatc ccgggcgtgg
    14101 tgggcatcaa aatgagcgac tggaatattt tccttctgcg cagcgtggtg ctggaatatc
    14161 cggaaaaagt ggtgtattct ggatttgacg agatgctggt gccaggcctt ttgtacggcg
    14221 cggacggatg catcggcacc tgggcgaatc tgctgcccga tttatatgca aaggtatacg
    14281 cgcgcgtgcg cgcgggcggg gcggacgccg tgaagccggt gatggatgag ttcaccgcat
    14341 ttctggccgt gggctggaac tacggcatta tcgatacatt tgaagagctg atgcgggcca
    14401 ggggctatgc cgggcggtgc ttccgcaggc ccagcgcatg ggagccgggc aaggtggagc
    14461 cggccgtatt ggccgacctg ctggcgcggc tgcaaaggct ggaggatatg gcggcggcgt
    14521 tatgagccgc ggaaaggagc gaccctatgc agtatacgaa gattcccggc acagacatgc
    14581 aggcaagcag gatatgcctg ggcaccatga ctttcggcag cccggtggcc ggacctgacg
    14641 cggcgcggct ggtgcggtac gcactggagg agcatggcgt caatttcatc gatacggcca
    14701 atatgtacga gggctacaac cgttatgcgg gcagcgcagg cggcgtggcg gaggagatcg
    14761 tgggcgaggc cgtggcacac cgccgcgggg attatatcct ggccacaaag gtgggcatga
    14821 aggtggggga ggccccggag gacgaaaata ccagccccga ggccatccgc attcagctgc
    14881 gccgcagcct gacgcgtatg cgcacagact atgtggatgt ttattacctg caccggtacg
    14941 accccgatac ggagccgcat gccgtcgccc gcgcaatggg ggaagagctg cgcgcgggca
    15001 ccatacgcgc ctggggcgtc agcaattaca cagcggctca gctggccgcg ctgctggcgg
    15061 cggcggcgga cgagggcgtt ccagctccgg ctttgtgcca gcctgccctg agtatgctga
    15121 ataccggcgc gctggaggag ctgctgccgc tgtgtgaaaa agagggcgtg ggcgtggtgc
    15181 cgtaccagct cctgcagggc gggatgctga cgggcaaata ccgccgggga caggaagcgc
    15241 ccgcgggcag ccgcctggcg gaaaagccgg agtggatgaa gcctttcacc gacgaaacct
    15301 atgatgtgat agagcgctgc gcggccgaag ccgcggcaga gggtgtgacg atgacacagc
    15361 acgcgctgcg ctgggcgctg gcacagcccg gcgtggtgag cgctctggtg ggcgtcaagc
    15421 gtgaagaaca gatcgacgag gcggcgggcg cggtgcggtg acgccggccc caaaaaaaat
    15481 gcggcgcgca caggcgcgca aaaggagagt gtgagagatg gcgtgtttga ccgacagcat
    15541 cccctggcgc ggggactttg agcaaaagct cgtttatgcg ccggaaaaac ggcccgggtt
    15601 tgtggcctgg gtgacggcgt ttccctacgg agacggttcc atcggtattt cgtttgacga
    15661 aacgctggaa gctgaaaatc cggatttcat cccgccccgg ctggaatttg ccgaggcggc
    15721 gggcgtgccg gtttcttatt gctccgtgga gggcggcagc gcgcggcagc gctcgtaccg
    15781 ggtgtatatg cgttctgtgg acggtgtgaa ttttaccgaa acgggccgct gcgggcggcg
    15841 ggagggttcg ctgtgcaacg caggctttcc cgacgggcgc atcgtgggct tcgacgtgcc
    15901 gcgctacaac gatgagcgca cgggctggag cgactgcatc cgcgtgcggg agagccggga
    15961 cggcggcagc acatggacgg atgtgcgccg gctgctggaa ggcactgcgc cctacctgtg
    16021 gcgcgtgcgc tgtctgcggg acggtacgct cgtggtgctg gccagcctgt acggaacgcc
    16081 ctggggcccg ggcagggagc gcgccacacg caacaccatg ctgcccggcg agacctacca
    16141 gagcaagata cagccgtttt ttctcaccag cagggatggc cgcgagttca gcgcgccgaa
    16201 ttacatcctg cccggtatcg gcgcccatga atttgatttt gtggaactgc ccgacggacg
    16261 gcttctcttc atcgcgggag acgtgcaggg cactccggtg gggcgccagt tcgtcacgcc
    16321 ctctccggac ggctggctga acggcacgct gtaccccatc tgcatgggcg cgcccgggga
    16381 cccggtgcgt gacccccagg gggggtatgt gcccgagacc attgcatggg acgcacagcg
    16441 gggctgcatc gtgggttacc ggcgcaacaa atgcttttca ctctccaacg attacggtga
    16501 aaattggacg cgcatcgagc cggacgtccc gttcgatttc ctctaccagc cctatctgct
    16561 ggcgctggac ggcgggcggc ttggcctgta tggccatgtg ggcggcgaca acgcttttgg
    16621 cgagaatgac atgaccatcc aggcgcaggt gtttgcaccg gagtgcgccc gcgatatgcc
    16681 gcgcgccgcg cagctctcgc tggagcggat gcttgcgccg gacgggagcg gatatatcaa
    16741 ttcgttccgc gcacgcctga cggcgggcgg ccgcccgctg gctgggcagg aggtggagtt
    16801 ccgcttcaac acctattgga acgaagacgg cagtgtaaac accacggcgc aggaggatgc
    16861 gccggataaa gaacaggtat gcacggacgc ggaaggctgg gctgttgctt cggcagtgcg
    16921 ctacaacggc atcggagaca tacacctggc ctacaatgtg gacgtagtgt gccatggctc
    16981 gcaggatgtg cgcgcctgca caggcccgat gatgacgatc ctggcgctga cgccgaggcg
    17041 tgaggctggt tatccctacg acgcttactt tgcgggcgga acactgtacc ttgcgccgca
    17101 gttcctgcgg gatttcccgc aggcggagga ggccctgcgc ggggcggcgg gcggctccgg
    17161 cgttctgccg caaggcgcgc tgtgtgaaga agccgtggaa cggctgctgg ggtgcggcgt
    17221 gctgcggcgc gggcaggacg gggcgctgcg gtggatccac agcgtgcatg cgccgcgccc
    17281 gctggacgac gtgcggccca tggcgagcgc ggactggtat atctgacggc agagccggtg
    17341 acttggataa aaagaaaaag aggggataca ggatggacta tggcgtgacc attacgcggg
    17401 gcgcggcccc gtggcagatc tttcagcagg ggccggacgg tacggcgtgc attcggctgg
    17461 agggaaaata tcatctggtg catctgtcgc aggagctgcc gctgcaattt tcggccgtgc
    17521 cgcatgcgaa aacgaccgtg aaggcccgtg tagcgctgga gagcacaggg gaatccgtcg
    17581 ttccgtggac ggaatgtacg gtgctggaca gcgaaaactg gacgataacc tttccgcggg
    17641 taccggcggg cgggctgtac cgcattgaga cgtatatgga ttacgagggc tgggacggcc
    17701 tctcctgcac acgcggcgat atggtgcaca atgtgggcgt gggcgatgtg ttcgtcatcg
    17761 cggggcagag caatgcggcg ggccgcgcaa aaaacccggt agcggacgac cctgagctgg
    17821 gcgtgcacgt gctgcgcacg agcgcccggt gggagcttgc aactcatccg ctgggcgaga
    17881 cgacgaacgc cctgcacgtc ggccattatg agaaccacaa cccgggccac agcccgtggc
    17941 tgcatttcgc aaagcggctc aagcgagagc tggggtatcc catcggcctt gttccatgcg
    18001 cttacggcgg cgcgccgctg cgctggtgga acccggagga aaacggcgcg cttttcacca
    18061 atatgctgga gatgctggca gattacgaca tccatcccag ggcggtgctg tggtatcagg
    18121 gcgaggccga gggctacgag gacagcgcgc agacatatct ggagcggttt gcggctttcg
    18181 tgcggcacac acgcgcggcg ctgggccagc cggagctgcc gttcctcact gtgcagctca
    18241 accgctgcat ggagggcccg agtgaaaagc tggaccgcca gtggggcatg gtgcgtgagg
    18301 cgcagcgcca ggcgtggcac acgctggagc atgtgaccgt ggtgccggcc gcggaccttg
    18361 cgctgtatga cttcatccac aatgcttcag agggcaatct tgtggtgggg gaacggtgcg
    18421 cccgcgccgc gctggccgaa tgctacgggc gcgatgtgga ctggatggcg ccggagccgg
    18481 aatccgtggt tcagactgcc ccggacacgg tgacggttcg cttttcgcgc atccgcaact
    18541 ggctcaatcc ctttggcgtg cccgcggccc tgctgccctt tgaagccgag gacgcgcagg
    18601 gccttgccgc cccgaaagcg tatgagacgg gcgcggacag cctgaccatc acttttgaac
    18661 gcccgcttgg cgcggatgcg cgcctgcatg gcgcatggcg catgaacccg ggcgcggcca
    18721 tcccaagcga ttgcatgcgc atgcccatgc tctcgttcta cggcgtgccc gtagaacagg
    18781 gctgactctt tttgccgcgc gcggcgcggc cgcatttcac agacaggagg gctgccgccc
    18841 tatgaaaaaa gatccatttc aatatatttt ccgcttctgc tgcgaccccg gcttcaacga
    18901 tgccgaggag atcccggcgc tgctgcgcta tgtggacgag gcggatatcg acgacgtggc
    18961 tgtattcgcc aacgtggagg agatcaacac cggccatatg agctttgacg agcaggacgt
    19021 gtatcttgca atgatgcgca aaatcagcgc gctgctggcc gaaaaaggtg ttacgatgtc
    19081 tgttaaccag tggcacagcg tcatgcacgc ggaccttggc aaaacgctgc ggccggagca
    19141 gaactttcgc cccatggtgg atgtggaggg aaatgaagcg aagctgtgcg tctgcccgct
    19201 gtgcggcgaa tggcagaaat acatcgcccg gctgtacgcg cgctatgcgg agctggagcc
    19261 ctccatcctt tgggtggagg acgacttccg tctgcataac cacgagcctc ttgtgtgggg
    19321 aggctgcttt tgtgacgagc atatgaagct gtacagcgcc cgcgcgggca agccgctgac
    19381 gcgggaagaa tttctggcag gcgtgctgcg tcccggcgag ccgcatcctt atcggaagat
    19441 atggctggat gtgagccgcg agacgatgct cagcgccgcc ggagccatcg gacaggcggt
    19501 gcgcgctgcg tcaaagcagg cgaaggtggg cctcatgagc tcggctccgc acatccacgc
    19561 cgccgagggg cgcgactggc atgcattgct gcacacgctt gcggccggac gcccgcctgt
    19621 ggaccgtgtg catctgcccg gatatcagga aaattccccg tcgaactatc tgcacggatt
    19681 caatatggtg tctatgctca cccgtgcaat gctcccatcg gagaccgagg tatacccgga
    19741 gctggaaaac tttccgttct cattgttttc gaaatcccgc cggttcacgc gtttccagct
    19801 gctgagcgca ctgccgctgg atctggcggg catcaccatc gacttgtatg acctcaacgg
    19861 caacggcatc gtttgggagg acggctatca gcagatgctg caccgtacaa agccctatct
    19921 gaatgccctg acccgcagcg gtgtgttcaa agaggagcgc ttgggggtac gcgtactgta
    19981 cagcccctgt tcttcgtaca cgctgcacac gcgggaaggc tcttccatgg aggagctgta
    20041 tccgcaggag gcgttttttg ccggcttgct gcccgccatg ggcgtgccct atgcttacgc
    20101 cggcagcccc gaactgacgg gtcagatagt ggcggcatcc ggccaggtgc tgcgcaattg
    20161 ggatgcggga acgctggcgc ggctgttcgc taataatttc gttattttga acggagacgc
    20221 ggcctggaca ttgtgcgaaa tgggacttgg ccgccttgcg ggcattgaga gcgtgcgctg
    20281 gctgcgccaa aacgacggcg gctacgctta tgagcaggtg acgaacggca agacctactg
    20341 cgggcgtaaa aacgcacggg cctcggccat cgtctcgtgc agcgacgcgc tggatgtgac
    20401 ctatgcgcag ggcgcacagg tgaacgaata tactgcgctg tacgattcct tccgccgccg
    20461 taccgcccac ggccaggtgg tggtggacgg ccgcgtactc atctatccct tcgggaattt
    20521 tgaaagctct gtatccatcc cgcccatgct gctgaacagc atgcgccagg ctgtgctgca
    20581 cgacgtgctg cgcacagcgg gcgcgccgtt tccgctggtg tgcggcgcgc cgtatctgga
    20641 gccgtactgc tttgtacggg acgggggatt ggatgtctac cttgtaaacg gcagcacgga
    20701 tgatgccgac gctgtggagc tggcgttttc tgcgggaact gcgccggaaa gcgcggaggt
    20761 ctggcgttcg cacgtagaac aggccgcgcc gcaggccgct gtgtgtgaag cggcgggaac
    20821 aggcgttcgg gtgccggtag atgtgcccag catggaagct gttctgctgc gcatgcccgc
    20881 gccgggggcg gaaggggaga ccccggcatg agcaaaaaaa taagggcatt tattggctat
    20941 ctgaaggtgc tggtgaacgg cgagacgctg ggcggcggat tttaccgttt tttgtcggtg
    21001 catgcgtgtt ttctggtatt ttacggcctg ccccatgtat ttatcaatac gatgctgttg
    21061 gggcagacgg gcgacgtaaa ggtcgtggtg atgtataacg ctacgttttt cctgggcagc
    21121 gcggcggcca tgctgctggc ggcagggctt ttgcagcgca cggattccgg cgtgacggcg
    21181 gtgctgggta ttttgggcta caatgtgctg tatctgctgc tgatcgtgct gggggacaac
    21241 gcctcgcgct ggcacttgtg gcttgggctt ctggcgggtc ttgcggacgg ctgctactgg
    21301 ctgagctacg gccatctgct gtcggattcc acagacctta ccaaccggga cagcggcctt
    21361 gccattgtga acatctgcgc caacgtcgtc aacctcacca tcccgctgct ggcgggtttc
    21421 atcatcgacg cagtgggcgg tacgcggggc tatatggcgg tgttcgggct gtcgtttgcc
    21481 gtgtccgccg tcacctgtgc gctggcgctg cggctgccca agcgccgtat ggcgggcgcg
    21541 cataaggtgg actatccccg cacgctgcgc gccattggac gcaaccggca tctgctgtac
    21601 agcctggctg cgcagggctg caaaggggtg agagaaggtg cgttcacgtt catcctcagt
    21661 atcgttttgt accagatggt gaaaaatgag ctgctggtgg gcttcaatac cttcctttca
    21721 gccgcggcgg ccatcgcttc ctacgtcatc gccagccgca tcctttcact ggcgaaccga
    21781 gtgcggtata tgggtatcgc ggtagcggtg ctcacaggca ttgccgcact ttgcatcttg
    21841 catctcagcc cgatgacggt tattctatat acagtggtca attcgttttt tgcgggcttt
    21901 ctggagaatt ccacatacag cacttttctg gatatgatgc agctggtgcc cgagatggac
    21961 gaccaccggc ccgagctgct ggctgtcaac gataccgttc tggagcttgg gcgctgtgtg
    22021 gggctttcca tcatcatcgt tatgaacgta tatgtgggcg aggatgtggg cgtgcaggtg
    22081 tggagcctgc ttgtgctgac gctcacccag gtgggcacgg tgctgctgtg ccgcggggcc
    22141 atgcgctccg cgaagcaggc cgcgccgggg gcttgaacgc gggcgttttt ccgcataaac
    22201 aaaaacatat ttgccaaaaa cggaagagcc gttggccgca aagcactttc cgttgttaaa
    22261 aaactgcgga aagaaaaccc tgccggcccg gcaacgcaaa gaaattcagg gcggctgctg
    22321 ccccagcaag cttcccattg ttaaaaaagg agaaatatga tgcaggcaaa gatcatagac
    22381 agaaagtggg tcgtcgagtg gttcgacgag cgccagtgct atcatatgcc cagcctgacg
    22441 ctggcggaca acggaaatct gctggccgta tggaacggtg gctttttgca gtggaacggc
    22501 gaccccatgg gccgcgacgc caaagactgg gtgtctgtgc tggagccggg cgcaaaagaa
    22561 tggtcgaacc cggacgccgt gggctgtgac atccgttatt gctgccatga cccggtgttt
    22621 ctcaaaaaca aaaaggggga gatcattctc ctgttcgcaa aatttctgga caccgaggtg
    22681 aacttcacca cctggtgcaa cggccgggac gagctgtgga cacgcaaaac gaaggacggc
    22741 ggccgcacat gggagccggc gcagcccgcg ggcatccaga gcggccacgc ctccaacgac
    22801 agcgtcctgc tggatgacgg caccatcgtg ttcgcctcca caagcagtga gcttccggat
    22861 aaatacttcg gcgcagttcg catctatctt tcccacgacg acggcgagac atgggaaaaa
    22921 ggccccatcc tctctgcgga cgacggcaat ctcatccggg agccggcgct ttgccttcgg
    22981 ccggacggca ccatccgtat gttcacgcgt acctgtccgg gcagtacggg ctggggcgcg
    23041 ggcgtgaaat cactggtgtc gtatacagcc gaaagccggg acggcggcaa aacctggacg
    23101 cagcctgtgc ccacaaccat cctcaataac gaatcgaaga tcgatgtcat cagctgggac
    23161 aaagacacca tcctgatggc ctacaacgac acgcctgtgg cagactggca cgagcgcagc
    23221 cctctgacgc tggcctattc gaaggatgag ggaaaaacct ggaaaaacct tatcgaactg
    23281 gccgccgcgc cgggaaataa atgccagccc gccatgtgca gggacaggga cggccgtctg
    23341 aatgtggtat atatgaaccg gcatacggcc attgaacacc tggtgctgga gatcacggag
    23401 taaaaacaga aagcgcggca cgctgtgccg cgctttgtgt ggttcacgcg ggccccgcgc
    23461 ccgcggatgc cgccggaaaa attgtggatt ttgcggaggg gaaacgcatg gcacagggac
    23521 aggccgccgc gccgccgggc gcggcggaaa cgcaggacgg cgcgcttttg gcgtgcattc
    23581 tggacatggg tgaactgctc ttgaccagcg gtgccgaggt gatgcgcgtg gaggataccc
    23641 tcacccggtt gtgtatggtg tatggttttg cgcaggcgga cgtttttacc atcacgtcca
    23701 gcatcgtgct gacggtgcgc acaccggagg ggcgcgccct tacacaaacg cggcgcatcc
    23761 gcgcgcggga tacggacctg ggccgtgtgg aacgggtgaa cgcgctctcg cgcagactgt
    23821 gcgcaggccc gctgccgccg gaaaaattcc ggcaggcggt ggaggaggta cgcaatgccc
    23881 cggcttaccc ggacgccgcc cagtgcgcca tgtacgccgt tatctcagcg gcgttctccg
    23941 tctttttcgg cggcacgtta cgcgacgctg cgacggcggc cgtttcgggc atgctgctgt
    24001 tcggcgcgct gcgcttttgc cagcggctgc ggctcaacgg tattttgcag agtatgctgg
    24061 ccagcgcgct ggcagcactg gccgtcatgc tgatggtggg cttcggcctg ggccagaacc
    24121 cggataagat catcatcggc aacatcatgc tgctcattcc gggcattgcg ctgacgacct
    24181 cgctgcggga catgatcaac ggcgatacca tttccggcct tctgggactg agcgaggccg
    24241 tgctcaaggc tctggccatt gccatcggct ttgcagccgt gctgatgcgg atggggggat
    24301 gaaaatggct tggtattttg tacagacgct tatgggctgc atcggttcgg ttgggtttgc
    24361 cgtgctattc aatatccgcg ggcgcaagct gctgctggcc gccggaggcg gcgcgttggc
    24421 gtgggcggtg tatctggcct gcacctgtaa cggcctggat atttttgcgg gcctgttttt
    24481 cgccacgctg gcggcggcgt tggccagcga gctgctggcg cgcgccgtca aggcgccggt
    24541 catcatgctg ctggttccca tgctcattcc gctgattcca ggcggcgacc tatattatat
    24601 gatgagcttt cttgtgcgcg ggcagtatga ggcgttcggc cagtatgccc agcgggtgct
    24661 caccgaggcc ggtgcaattg cgttgggcat tatctgtgtg gcgtcgctca tgaatatcat
    24721 cgcgggcctg cgtgtgcggg cgcggggcgg caaatagagc gcttcagggt gtgccgcggc
    24781 ttgaaaaaac catccgtatc ctgtatgata aaaggtatat tccgcatgag atacaagcat
    24841 gcaggaaggg agaatgcaat gaagatcatg gcagtggatt acggcgatgc ccggacgggc
    24901 cttgctgtgt gtgaccgcac cgagttcctg gccagcccca tcggcatcat cgaggagaag
    24961 agcctggcca aggtggcgga aaaaatcgtg tacgccagcc gcgaatatga ggtggggatg
    25021 atcgtcatcg gcctgccgaa aaacatggac ggcagcgagg gcccgcgcgc ccagaagagc
    25081 cataagctgg ccgatacgct gcgggccatt gtgcccattc ccgtggagct gtgggacgag
    25141 cggcagacga ccgtaagcgc ggcgaacatc ctttcggaaa acggcactta cggcaaaagg
    25201 cgtaaggatg tgctggacgc ggtggcggcg acgg
//
LOCUS       DAWBSB010000006        22904 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_602_length_22904_cov_3.759902, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000006 DAWBSB010000000
VERSION     DAWBSB010000006.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 22904)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 22904)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..22904
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_602_length_22904_cov_3.759902"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2526..2535
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    17640..17649
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    20089..20098
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    21430..21439
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4270 a         7172 c         6731 g         4691 t
ORIGIN      
        1 gcatggttca atgttctcaa agccctgtgc gtcagcacag gggcgtgtgc catgcgcacg
       61 cgcagccgcg caagcggcgg ctttgaggtt attataccat atttccattc ccccgcccaa
      121 cggtggaaaa gcgcggcacc gggcgcgccc catgcaccga acacaggaca cgcccgcccg
      181 ccgcgcgaaa aaggggagca cctgtaaaat gcgccgcaaa agctgtcagc ccttcaccgc
      241 gccggcagtc atccccttaa tgatatagtt ctgcaggaag atatagacca ccagcaccgg
      301 aatcacgacc atcaccaccg cggccaccat caggccgtag ttcgtgcccg cctggctgga
      361 tatctcgttc agcaggcgcg tgatggtgaa gaattttttg ctgcgcagga aaaacatctg
      421 taaaaaaagg tcgttgtagc tccacagaaa cgtgaagatg cccaccgtgg caaagctggg
      481 cttggccagc ggcatgatga ttcggaagaa tatctggtag acgttgtagc cttccagata
      541 agcggcttcc tccagatcca ccgggaggct gcgaatgaag cccatcagca caatgatggc
      601 aaacgacagg ttgcccgcaa tctgcggcag gacgaccgag agccgcgtca gccaaatgct
      661 gcctgtattc acgatgcccc aggacgactc catgcggaac accgggatga tcgtggagaa
      721 caccgggaac atcatgctgg cgatgatcat ggaatgcaga aagccccgca gccggaagct
      781 gtaccgcacc atagcgtagg cggcaagccc agccagcacc atcaccacca cagtgacagc
      841 accgctgatg acaatgctgt tgacataggc cgtgccgatg tcaacgcgct tgaaggcggt
      901 gacgtagttc tccagtgtga atttgtcgcc gatgggcagc gagaatgtat cgctgcggat
      961 cagcccgcgc ggcttgaagg aattgaggat gacccagata aaaggataga tcgtcgtgag
     1021 cgcccacagg gacataaaca catagaggag cgtttttccc acgggaaagc gtctgcgtgt
     1081 cataaaaaag ccccccttaa taatcttttt ctttgaagat gcggttcgcg atctggctga
     1141 ttacaacacc cagcaccacg atgaggatgg cgatggccga gccgtagtcc acgtcctgca
     1201 gcaccatagt ctggtagtac atatatgtgc cggtcagcca agatttgtcc agcggcaggc
     1261 cgcctacaaa catggtccac ggcaggtcga acaccttcag cgagccggta atggccagca
     1321 ccaggcaggt acacagcgtg ttgtgcagca gcggcagggt gatataccgg atgcgcccca
     1381 ggccgctggc cccgtcgatc tcggccgcct cataaatatc ggtagagata ttgttcagcc
     1441 ctgtgacgag aataacaaaa taaaacccca catactgcca gataacaggt accatcatcg
     1501 tgacgaaaaa gtgggcgggg tccttccagt cgatattctc cgcggcgagg cccaggttct
     1561 gcagaagctg gttgagcatg ccgccgtcgt acagaaagat caggttgaac agaaggccca
     1621 gcgctgtggc gctgatgacg atgggaaaaa aatacacggt gcggaagaat ttcgccccca
     1681 ccttgatatt gtccaccatc agcgccagca ccagcgcgat gcccacctga aacaccacgg
     1741 tcagaatcat cagaatcatg gtgttcttca gcgcggtgcg catgagcggg tctgtgaaca
     1801 tcttgacgta gttctcccat accggctcgt tcaggccgcg gctgctctcg cccaggccgc
     1861 cgatcttgtt gaggctgttg tagatattca tcaaaaacgg atagtataag tacatggtca
     1921 aaaagaggaa ggtcggcacg aggaacagca cgagcgggcc tttctttttg tagatcatcg
     1981 cattcatggc aaggctcccc ttcttttctg cgtttgtctg cgggatgcgg gcgcgcgaag
     2041 cgccgtcgtt tttcggacgg acgcgccgcc gttcggtgcg gcggacggcc ccgggggcgt
     2101 gcggcacggc atgcgccgcg gccgaaggcc cgttttttca aaaaaggccg gagagccgtt
     2161 tccaagctct ccggccgtat tggctgctta tatcgcttgc gtcaggaatc tgccagcggg
     2221 gtggcgaggg ccgtctcaat ggcctcctcc ggcgccaggg tgcctgtcac aacatccttg
     2281 acagaggcga acagaacgcc gcgggcagtc tggttcagcg catcctgcac ggcgctggcc
     2341 atgcctgtct tgccgttgaa catggtgacg gccgccgcgt gcaggctgtc ggtatccgcc
     2401 gcttcgggca ggccggattt cagcgccgta ggggcaccgc ccgcgaactt gttgaccata
     2461 tcgtcggtgg tcatgtattc cacgaactgg acggccgcat cgcgcttttc ggggtcgctc
     2521 caggcnnnnn nnnnnccagg ctttctcggt gatgtagtag cccatggaaa tgccgcccac
     2581 gatgtccgtg gccttgcggc tgccgctgcc cggcacataa gtaacggtgt aatcgtcgat
     2641 gttctccacg ttgcctgcgg cgtcctcgct gccggtgaac cagcccacct tccacgagcc
     2701 gtcaatggcg aacgcggctt tgttgtcggc catcatctgg aatgtgtcgg cgtcgcccgc
     2761 cgtcagggta tttgcgggga aatagccctt ttcgtacatc tctttgaggg tgttcaggcc
     2821 cgcgacccag cgctggcccg cttcgtcggc gccggaagca gggacctcgg cgtgcttggc
     2881 cacgtcgccc tggttcagga tgcagaactc gaaccagtag tgcggcacct cctgcaggga
     2941 gcaggcgatg ggcgtatagc ccttgctttt aatggtctca cacatctcca tgaactcgtc
     3001 ccaggtcgta tcggggccgg ggacctccag gccgcagtcg gccagaacgg ttttgttcac
     3061 gaacatgcct tcccaatatc cgctggacgg aacggcgtag gccttggcgt ccacaaagga
     3121 aaggggcatg gccgcatcca gcatgttgga tgcatagtcc gggtattcct tgcggatctc
     3181 ctcgatggac acgaccttgc cgttcttgat aaactcgctg gcgtcagtac ccgtgaagaa
     3241 gaagagcacg tccggttcgc ttccggtctg gaagtcggct ttgacctggg ccttccactg
     3301 ctcgtttgag gtggcggatt cgtctaccac agtgttgccc gtggccgctt cgtaacccgc
     3361 caccgcttct tcataattgg cgcggttgcc gtcgtcgccg ccgtaggacg tcacgacgcg
     3421 gatctcaaca ggctttgctt cgggggcgga ggagcctgtg cttccggcca cggacgcgga
     3481 agccggagcg ctggagccgg aaccgccgca ggcggccagc atggaagcgc cgaggcacag
     3541 cgcaagaatg atcgcgagtg tctttttcat gtttcaaacc tcccattttc tgggggcatg
     3601 cgcggcgttc acagcaccgt ccgaatgcct ccctgtgcct ataatgttat accagtttgc
     3661 acaaaaactg aatgaacgaa cttgtttttt attgcatatt attgcccgtc ttgttcaaat
     3721 ttgatattca ttctcgacag cgtattccct ttttcagtga ttgtgatgga aaggccgctc
     3781 tcctgcccgt agatgatctt tacccgctcg tgtacgttgc ggatgcccag cgaagcgctg
     3841 cccgttccct ccggcagcgg ctcgtccgag agaagcgtct ggatcttcat caggtcgtcg
     3901 taggtggtta cgccgtcgtt ctcgatctcc agcagcagcc tgtcctgctc gcggtaagcg
     3961 cggatgatga tggtgccttt ctgcacggga ttgatgccgt gctccacggc gttttccagc
     4021 aaaggctgca gcaccaggcg cggcacatag cagtccagcg tttcggggga gatctccttt
     4081 tccacggtca gccgcttgcc caaccgctcc cggatgatga acaggtacgc gtccacatac
     4141 atcatttcct cggagaggtg gatgagcggc cggtgcttgc ggtccatggc cgcgttcagc
     4201 atcgtggaca gcgcctccag catctgacat accttcacgt cgtcggccag gcgcgcttcc
     4261 cagttgatga tctccagcgt gttattcaga aaatgggggt tgatctggct ctgcagagcc
     4321 atgatcttcg cgtcccgcag ggccagctcc tccttataga tgcgctcgaa ctgatttttc
     4381 agcttgtcgc tcatggcgtt gaagctgttg cccagatagg cgaactcctt gctgcccagc
     4441 cgcccggcgt ccacctgaat gccgaattca ccctgctcga tgtgcgccga agccccgctc
     4501 agcgtgacga tgggccggtt taccttgcgg tacagaaacg cgatgaccgc tcccatcagc
     4561 ggcagcagcg cggccagcag cgcggccagc agcaaagccg tgctgcgcag ctgggcgcgc
     4621 atggccgcgg tgtcggagta gacgatgtat tccagcgcac agccgggcac tacgctttca
     4681 ctgccccaga cggcgtagct gccctgcgcg ggcgcgccgc cctggccacc cgcacggatg
     4741 ttcccgcgca gctctcccgc atcccgcacg ctgcccgcaa cggtatactc cgcgtcgccc
     4801 agccagaccg tagcgtccgt catccagatg acactgcgca tactgtcgaa cagcgtgtcg
     4861 gtgttcagct ccatcaccag cacgccgtag gtgcggtagt tggcgtccag cagattgcgc
     4921 atcatgtaca ggctgccgtc cgccgcaaag aaaccgatgc cggtgcccag cgcttcgctg
     4981 cggaccagta cttcgtcccg cacatgctgc tgatatttgc gtatggaggt atacgaggtg
     5041 ttggaggtat agatgggcgc tgctggatcg tccaccagcg tgacgatggt attgcggaat
     5101 ttgtcgtcgt agcggtactg ctgggtgaga aaggtctgca tctcgttgta aaatgcgacg
     5161 taatcgcccg ttttcgtgta ggatgcccag gcattgccca tggtggcgat ataactggcc
     5221 ttacggctgg cggcgatcat ggaatccagg ttgcgcccgg tgatggccgc ggcgctttgt
     5281 gcgctgcgca tcatatcact gcggtactgc cgcgtcatgc tgtccagaat atatacaccg
     5341 cccagcgtga cgatgagtac aacgggcagc acccagcaca gcaggataat gcccagcatc
     5401 ccctgccgca ggccgagctg ccgcctgtgt ttcattttct gccgttccat gcctccgcct
     5461 ctttcccggc gcgctttgca agcatccgcg cgtcctgccc gctattatag caaaaaaggg
     5521 ggacaatgtc cccctttccc cgcttttcgg ccgcttttcg ttacagcgac aaaaactccg
     5581 tcaaaatttt ggcaattttt tccgcatttt ccatggtatc catctcacag aacacgcgca
     5641 gcagcggctc ggtgccggaa aagcgcgcga tgacccagcc gccgtttttg aagtacacct
     5701 tgcagccgtc catgtagctg accttggcga tctcgcaagg gaattccggc agctgtttgt
     5761 cctccatcag cagcttgaag atgcgcgtcc tttcggcgct gctgaaatga aaatcgtgtt
     5821 ccgccatctc ggcggcaccg tatttctgcg tgatttcgtc ccacagcgcg ctgagcctgc
     5881 ggcctgtttt ggcaatggcc tcaaccagca gcgcggcggc ataaatgccg tccttgccct
     5941 ggatgtgccc gcgcaccgtc agcccgccgg agctttcgcc gccgatgatg gcgccggtct
     6001 ccaccatctt ggcagacaca tatttaaagc ccacgggcac ctcgtagcag gtctcgccga
     6061 agtcctcggc aatgcggtcc agcagatggg tggtggccag gttccgcacc gctgcgccgc
     6121 gccagccgcg gtaccgcatc agatagtaat acagcagcac cagaatctta ttgggatgca
     6181 ggaactccgc cttatcgtca atgacaccca ggcggtccgc gtcgccgtcg gtggcgatgc
     6241 cgatgtcgca gccgtgttct tccacatagt tcgtcaggcc cgccagcgtc ttgaggttgg
     6301 gcgcgggcag ccggccgccg aacagcgtgt ccctccgctc atggatgacc tctacgtcgc
     6361 accgggtggt gagcaagatg gtctgcagcg ctgtcttgga cacgccgtac atggggtcca
     6421 gcaccacgtg caggccgcgg gaccggatgg cctctgcatc caccattttg aggatgctgt
     6481 cgatgtagtc gttcatcggg tcgatgagct ggatactgcc cgcagcaagc ccttcttcgt
     6541 agggcaccgt ctccacaggc tcgccttcca gctcgcggat atagccctca aaggcggcgg
     6601 taagcgtttc gtccgcgtcg cgcccgccct ccataaacac cttgatgccg ttgtagacgg
     6661 ccgggttgtg gctggccgtg atggccatgc cgtagggcag gccataggca gccaccgtat
     6721 acataataag aggcgtgggg ctttcccggt ggatgatgcg gcacaggatg ccctgtcccg
     6781 ccatcacctc agcggcccat ccggcggcct cctcgctcaa aaagcgcctg tcatagccga
     6841 tgacgaagcc cttgtcggcc ctgccctcat ccagcatttt acgcgccagc gccagcgtca
     6901 gcagctggac gttggctttc gtgaagccgt cgccgatgac ggcgcgccag ccgcccgttc
     6961 caaaatggat catcgtttcg ctctccttcc tgcatatccc cgccggggaa cctgcggcgc
     7021 gcgtcagccc agcaccgccg tcacgcggcc cgtcgtgccg ggcgccagca gcggcagccg
     7081 ttccacacgc acaccgtcca ggtacagcgc ctttacgccc ttgcacacat gggcggggtt
     7141 ccgcacctcg atctcataca ccgcgccccg gaaccgccgc acggccgtaa agccgtccca
     7201 accgccgggg atgcacgggt cgatgacaag gccgtcgaac tccggccgga tgcccagcat
     7261 atagcgcgtc gcggccacat agctccaccc gccggagcct gtcatgaacg gatggtgcgc
     7321 ctcgccgaat ttcttgtggt cgcgcccggt gatgaactga cagtaggaat aaggctccgc
     7381 tttgcgcgtc tctattttgt cattctgcca atagggacac agtgcgtcgt aaaactgcat
     7441 ggccctgtcg ccccgtccca gcacgcattc cgccgcccac gcccatgggt tgggatggct
     7501 gaaaatggat ccgttttctt tcaggccctt atacacccgg gtgacaaaac caatgtcgtc
     7561 gtcgggcttc gagtaagccg gcgcgttgag catcaggccc cagggcgtgt agaggtattc
     7621 ctccacgctg tccatggccc gcgcgccccg ctccggccct gccgcgccgg agagaacggc
     7681 ccaggtgttg ctctccagat gcaccttgcc ctccgtatcc gcgtccgttc cgatggggcg
     7741 gccgtttttt gtgaagccgc gcagatacca tctgccgtcc cacagctctt tttcacacac
     7801 gtccgccacg ctgcgccgca cggcctcgta atgctccgcg tctgcctttc ggcccaacca
     7861 gcgggccagt gcggcaaagt ttcccagtgc ccaaacgtgc agaaagctca ccatcgcgct
     7921 ttcgcccccg cccaggttca ggcaatcgtt ccagtccgcc cgcaggccct ggcagatccc
     7981 atgcgcaccc accatgcgct gggaaaaatc caggatcgtc ttcaggtggc cgtaaaccgt
     8041 gtccgtccct ttatccgcat agggcaccgg catgtcaagg aacgcggcgt ccccagtctc
     8101 ccgcacatag tccgccacgg cggaaacaag ccacagcgca tcgtcgctgc atgcgtccgc
     8161 caggccgtgg atcatactgc ttttatccgg cgtgggcaca acggtcgggc tcttgaaact
     8221 ctgccggggc gtctcctcga accattccgg ctcgaacaga tgcaggccgt atccctcgct
     8281 gacaagccct tttaaaagct gcacgatgcg ctctttgcag cgggcggggt tcgagtgcag
     8341 cacggtcatc gcgtcctggg cggtgtcgcg gtagcccagc ccggtgcggc cgcccacctc
     8401 gatgaagctg gcaaagcggg aaaactgcac gtttatctcg ctctgataca gcgtccagat
     8461 attgatgagc gtgttcatgc cctcattggg cgtgtcgatc tgcagcgccg cgcatttttc
     8521 gtcccagtag tcgcgcaggg ccgccagtgc cgcgtcccgg gcggcgggcg gcgcatattt
     8581 tttgcggatg cgcacgcctt cggcccgcgg cccctccccc aggaaaaacg cttcgcgcag
     8641 gacctcgccg gacgcgagga taaaacgcct gtgcagcgac gcgcactggt tgccgcccag
     8701 ctccgtgctg ccggaaagcg tgccccgctc cacgccgatg gggtctgtct cggtgcggta
     8761 cgggccgatg aacgcctccc gcaccgtatc gtagctgtcc ggcgggaaag tggccgcaaa
     8821 atactggaag ccgttctcct catagaaaag gtcctgttcg atgatgccgt ccgcatagct
     8881 gccgcccgcg gcatacaggc tcatctgaaa gttgcggttg tccatatcga tatggtggaa
     8941 cgacagctcg gcgtagctga acacgctgat gttccgcgcc cggccggaga tattctccaa
     9001 cgaaaggtcc cagatctcca cagcctcgcc ccggggaatg aacagcgtct gcacggcgcg
     9061 gatgccgccg tactcacatt cgtattttgt ataggacaag ccgtggcggc aggtatattt
     9121 cgcccagtcc agcggcttgc ccacgggctg ccacgaaaca gaccagtact ccccggtgtc
     9181 gtcgtcccga agataaatgt aatggccggg ccggtccatg ggcacggcgt tggcgcgaaa
     9241 acgcgtgatg cggtgatatt cggcgctttt atggaacagg tatccgcccg ccgtgtggtt
     9301 caccacggcg cacatctcct ccgtgccaag ataattggtc cagctcgcgg gcaggtccac
     9361 cctgtcgatg acatattccc ggcggtcgtt gtcgaagtgg ccgtactgca tactgcatcc
     9421 tccctgtacg ggccgcgcgg agggcgcgcc cggtgtcgtt gcttttattg tacggcgcgc
     9481 ggcgcgcggt gaacagggcg gggcttggca tatatttgcg tttcttgcca cgtggcgccg
     9541 gccatccctt tctccgggcg gcggtgtctc tgctccatgc gggccgcacc ggtcatcggt
     9601 tttacgcacc caaaaagtca tttgattttt atcgtcattt ttatattgac ttcgcctcgc
     9661 aaaaagtctg tcaaatgcgt gcagattttt tcgacttgtg accatgttgt gaccagacgg
     9721 tggtatactg agtataaatc agtcacagaa agcccgaaat tcgatacact caggaggaat
     9781 acgatggaaa cgaaccggat gatgtctccc gccgccaggc ggcttgcggc agttgtgctg
     9841 gccgcgctgc tttgtctctc gctgttctgg cccaagggcg gctcgctgac cgccgtgctg
     9901 tgcggcgctc cattggcctc cgtaaacggg cggcccgccg cgcaggtgat ggcggtgcag
     9961 gcggagcaat agccttttac gcagaaatgc cggcggcgga aatttccgct gccggcattt
    10021 ttgattcttt tccctttttc gccctggctc accgccgtct ttccatcacg aaattggtga
    10081 gcgccacgcc ctgccgttcc acctgctgct cccggatgac ccgataccct cttttttcaa
    10141 aaaaaggctt cgccgtaatc gatgcgtggg tgctgataac gcccgcgcac acctgctcca
    10201 gcctgtcgca aagggcggtt gccacgccct tgccctgatg gcgccggtgg acgtacaaac
    10261 ggtccagata tcccgagccg tcgatatccc caaacccgat gaccgcaccg tcctccaaag
    10321 cgacaagggt atggtgttcc cggaacgagc ggtcccaggc ctccagatcc acatggcccg
    10381 tcgcccacgc gtccagctgc tcctttgtat aatccccggc attgaccgta tggaccgtgt
    10441 cgtagaacag ctccgccaat tcccggcaat ccgacggcct gtattttctg atctgcatgc
    10501 ttatcctccg catcacggcg cctcacagcg cgtactccat ctggcagtca aacggctccg
    10561 acgccacatg ggcctcattg tactcccggg cctccacgca gcccatcgcc gcgtaaaacg
    10621 cctgtgtctc caccgccgaa tgggccgaca gatacagctt ttttccgccg tgcgcacggg
    10681 cccactgcgc cgcccgggta aaaagctccc gcccgatgcc tctgccccgc agctcagccg
    10741 aaacgtgcag gcacgtcagg tcccggtaat cccccgcttt gcccagcggg gcgctttcca
    10801 ccgaggcaaa gcccttgagc gcgccgtccg caaaggcccc cagcacaacg ccgcccgtgg
    10861 ccagcgtatt ttgcaggcat tgcacaagaa acgcataatc cgcttcgctc cagtcgtcga
    10921 tgaacggcgc gtcccgcacg acccatacgc cgttttccct gcggcggcac ttcgtcactt
    10981 cctggcgccg ttcaaaatgc cggaacagcg ctcggtccag ctcgccgggt gcgatctcgc
    11041 ggtattccat atccattctc cttctatgcg gccgccgcgc gccgctcaaa acacctggaa
    11101 caggtaaaac ttcagataat tcgtctcgtc cacaccccac aggatgggat ggtccggcga
    11161 ttgctggcgc gcctcgatct ggcgcagctg cacgcccgcg tcacgggccg cgtctgccag
    11221 catggcgcgg aacatcccgt ccgtggcaaa atggctgcag ctgcaggtgg caagataccc
    11281 gccccggggc agcagcttca tggcgcggta attgatctcc ttatatcccc gcagtgcgcc
    11341 cggcactgtc ttgcgggatt tcgtaaacgc gggcggatcc agaatgatga aatcatactt
    11401 tttgccgccc tcctccgcca gacggggcag caggtcgaac acattggccg ccacgcagtc
    11461 cattctgtcc gacactccgt tgatatccgc attgcggcgc gccatctcca cggcggcctc
    11521 ggagacgtcc accgccgtca catggaccgc gccgcccttg gctgcgttca gtgcaaagga
    11581 gccggtgtgc gtgaagcagt ccagcaccgt cctgccctgc gcaagccgcg ccacagcctg
    11641 ccggttgaac ctctggtcca gaaaaaaacc cgttttctgg ccattttcca cgtccacagt
    11701 atagcggacc ccgttttcca caatgtccgt cagacaggtg tccggccggg gcaggcccag
    11761 cgccgaaagg tcaaaccagc ccttgttttc ctccagcccc tcccgggcgc ggatggcaac
    11821 gtcgttgcgc tcgtacaggc cgtcgatctg ctggccgtcg tcccgcagca gctttaccag
    11881 cagcggaaag agcatgggct tgatgcgctc gatgcccaca gagagcgtct gtgccaccag
    11941 ataccggccg aaacggtcca ccgtcaggcc ggggaacagg tcggcctcgc cgaagatgac
    12001 acggcagctc gaaacctccc cgctcagcac tgttttgcgg tactcccacg cgtgggacaa
    12061 tcggcgcagg taaaacgcct cgtcgaagac gtcattggca ttgcgtgaaa ggatgcgcac
    12121 acgaatcttg ctgtgcaggc tgatgaagcc cgtgcccaga taccgtccct ttgcgctgat
    12181 cacgtccacc agcgcgccgt tttccgcggg ctgcgcgtcc gccacttccg tatcgtacac
    12241 ccacggatgt ccacccgtca gcccgcgttc ggccttgggg gtaacggtca cccggggata
    12301 tcctctttgc tgcttcatgg tctttccgcc ttttcttttg ggtgcgcacg tttgcgcccg
    12361 gccgggcttt ttacaaagcg cccggcattt ttgacttgtc ggatacacac tattataatg
    12421 gtcttacgaa aaaaaagata gtggggtgtt aaaatggaga tcgaacgcaa gtggctcatc
    12481 gacgggttcc ccgccggtct cccgctgctg aaagaggccc gggtgcgcca gggatatatc
    12541 tcgacagccc ccgtggtgcg catccgggag agcgtgaacg ccgacggctg ccgctgtgtg
    12601 ctgtgcttca agggcgaagg cacgctggcg cgggaggagg ttgaaacgga tatttctgcg
    12661 gaattgttcc ggcggctcac agccttcacc gggcaggacc ttgtgaccaa ggattacagg
    12721 gtgtatgcgc tgcccggggg cgagcggctg gaggtgagcc tggtggacgc gggccggccc
    12781 acggcgtttt tctacgcgga ggtggagttc cccaccgtgg aggccgcccg cgcctttgtt
    12841 ccgccgccct tcctgggcga agaaaagacc gagagcggcg atttttccat gagcgcctac
    12901 tggcacgcta cccgcggctc cgaccgggag ggatagccat gcacacgggc cgcctgttcg
    12961 agatcgtata tctgctgcaa agccgtcccc gcatgacggc gcgggagttg gccgaacgct
    13021 ttgaagtgag cgtgcgcacg atttaccggg acatcgacgc gctttcggcc gcgggcgtcc
    13081 ctgtgtacgc ggcgcggggc ggaggcggag gcgtggggct gctgcccggc tatgtactgg
    13141 accgctcgct gctgacggac ggcgagcagg acgagatcct atatgccctg cgggccatgg
    13201 ccgccgcagg tatgggaggc gcggcgctgg aaaagctggc cgggctgttc cagaaaagcg
    13261 gcgacggctg gctggaggtg gatttttctc cctggggctc cggcccggcg gaaaaacgcg
    13321 tgttcgccac actgaaaaca gccatccttt cgcgccgtga gatcgccttc gattattaca
    13381 gcgcgcgcgg cgaaaaaagc cgccgcacgg cccagcccgt gaaactggtt tataaagatg
    13441 ccgcttggta cctgcaggcg tactgcctgg cacgacaggc gctgcgtgtg ttcaaactgt
    13501 gccgcatgct ggacgtgacg ctgctggacg ccgttttcac gcccggcccg cagcacacgc
    13561 cccagccgat ggacccgacg gaggcccccg gtccgctttt gcatctggag ttgatgttca
    13621 cgcccgccgc cgcgtaccgc gtttacgata tgtttccgcc ggagtgggtg acacagctgc
    13681 cggacggacg cttcttcgtg tccgcctttt accccgaaag cccgtggata tacggttttt
    13741 tgctgagctt cggagccgat gtggctgtgc tgtccccgcc gcaggtgcag caggccctgc
    13801 gcgaggccgg gcagaaagtg gcggcgctgt acggtgcgcc ggagtgaccc tttggcgcgc
    13861 ggccggctgc ggaaaattta tttttctttt gaatatgacg caggatgtca tcttccccgg
    13921 tttatactga aggtacaaca accgaaaggg gaatgcaaca tggaaaacca gaacgtcaag
    13981 cgcttttgcc agagctgtgg catgccgctg caaaacgagg ccgactatgg caccgaagcc
    14041 ggaggcgcca ggtcggagga atactgcacc tactgttacc agaacggcgg tttcacggcg
    14101 gactgcacga tggacgagat gatcgatttc tgcatccgct tcgaacagca gcagaacccg
    14161 gggctggacg ccgaggacgc ccgccgcgcc atgcgcgcgt ggtaccccac gctcaagcgc
    14221 tggaagcagg cgcagggctg acgcgtatgc ggcgcgtcgc tccggcccgc gcgggggagg
    14281 gcactgtacg ggaaaacacc gcgccgccaa ggccgcacga actccacacg acgcctcttg
    14341 gcgccgcgcg tctgtgcaaa aatctcgggt ttgcacccca ggacgtcatg cggcagtgca
    14401 tacggtgcat cgcacagcca gatgcggttt atcagcgcag gggcaaaaac tggtatatca
    14461 catctgcggg ctgcgaattt gtggtcaacg cgggcagcgg cacgatcatt accgcacacc
    14521 gccgcccgcc gccgcaaaac ggctcttgac aaaccgtttc cgctttgcta tcttaaaaaa
    14581 cagcagctac gctgctgttt tctacgcgca tccaggcact ttgtgcccgg gcgcttgaga
    14641 tcttaagacc atgggccggg acagcccaca tggctgcttt ttatagcttt gcacggcaaa
    14701 gctataaaaa aaatagctgc gggctatttt ttcggctgtg cggcccgtcc ccgggcgcgg
    14761 ccatcaagat cgttttttat tgggagcaat aaaaaaaaat cgaacagacg ggagatggaa
    14821 ggatgcgcat ttgacctgct ttttgtacgt gcacaaacct gcggcgcagc gttttcgcgc
    14881 gctgctgtgg tttggtgcgc cttccacaaa gcgggaaaat gcgtttcgtt ccggctcctt
    14941 ccgtgtgtaa ggaagggcag ggccgccttt gtgcggcgcc tgcctttttc agggtttccg
    15001 gcgcttcggc ccgattctgg acacatggtg gaaaaagccg cgggcgcgtt cccgcggcct
    15061 ttttcgattg gagttgaagt atatgtccca aatcaccgtt tcccatctta ccttcgccta
    15121 tgacggcgcc tgcgacaacg tgttcgagga cgtctctttc gtgctggaca ccgactggcg
    15181 gctcggcttt accggccgca acgggcgcgg caaaaccaca tttctgaagc tgctggacgg
    15241 cacgctggat tcccgcggcg ccgtgcgtgc cggcgtaccc gtgctgtatt tcccattccc
    15301 tgtggaatat ccggaggacg atacgcaagc cgtgctggaa cggctgctcc cggattgccc
    15361 ggcctggcgc atcctgcggg aactgaatct gctgggtgtg gagcaagacg tattatatcg
    15421 cccattttat acaatgagta atggagagca aaccaagatc ctgctggcca gcctgttttt
    15481 gcacgatggc gcattcctgc tcatcgacga gccgacaaac catctggatg ccggaggacg
    15541 ccgccttctg ggtgaatatc tggcgcagaa aaggggatat atcctggttt cccacgacag
    15601 agacctgctg gaccgctgcg ccgaccatat gctggtactg aaccgccgcg acatcaccgt
    15661 gatgcagggg aatttcaccg tctgggagga aaacaaacgc gtgcaggacg accgcgaacg
    15721 cgcggaaaac gaacggctgg aaaaggacat cgcccggctg ggtgaggccg cgcgccgcac
    15781 cgagcgctgg agcggcaggg ccgagcagga aaagcacgcc cgcaacagcg gcctgcggcc
    15841 ggaccggggc tttctgggcc acaaggcggc caaactgatg aagcgggcca aagtcaccga
    15901 agcgcgccaa caggcggcca tcgcagagaa aaaaacactg ctgcgcaatg tggacgaggc
    15961 cgacggcctg aagctgcacc cgctggcctg gggcaaaagc gggccgctgg tgcgcgccga
    16021 aggcctgagc ctttcctacg gcgtgcgcac ggtgtgcgca ggcctgacgt tcacgcttcg
    16081 ccccggcgag cgcatggctg tcaccggccc caacggctgc ggtaaaagca cgctgctgaa
    16141 aaagctgtgc ggtcaggacg tcgccgcctg cggtacgctg gcgctcggcg cggggcttat
    16201 tgtgtcttat gtgccccagg acgcatcatt tctgcacggc ggcgcacgag actacgcccg
    16261 gaacgcgggc gtggacgaga cgatgttcct gacgctgctg cgcaagcttg gctttgaacg
    16321 ggtgcagttt gaaaaggata tggcggatta cagcgccgga cagaaaaaga aagtgctgct
    16381 ggcccgcagc ctttgcgaaa gcgctcatct ttacctgtgg gacgaacccc tgaattatat
    16441 cgacgtactg tcccgcatgc agatcgagca gcttttactg gactaccggc ccacaatgct
    16501 gtttgtggag catgacgcgg cctttgtgcg ccgggccgcc accgggacgc tggcgctgta
    16561 agcaggtgcc ccatcacagg aaagaaggaa tttttatgga gcttaccatc cgggcggacg
    16621 tttccaccca tatgcgggaa ttgaagcaat ggcttgcaca gacgcgggat gagcccgccg
    16681 aggatatgaa cggctttttc gccgcccgcg tggacaccta tgaagcgcac atgtccgtgt
    16741 gggacaaagc atatggccgt gtggccgagc tgacgcccgg agactgcggc cccctgctgg
    16801 acctcggctg cggcaccggg ctggagctgg accgcatctt tgcccgctgg ccggagcttg
    16861 ccgtcacggg agtggacctg tgcgcggcca tgctggaaaa gctgcgtgcg aagcatgccg
    16921 ggcggcggct tgagctgcgg tgcgcggatt atctggcgat ggacctgggc gcggagcaat
    16981 acggcgcggt cgtttccgta gagagcctgc accattttac cccggagcaa aagctggagc
    17041 tgtacaaaaa aattcatgcc gccctgcggc ccggcggctt ttttctggag gcggactaca
    17101 tcgcctgctg cgaggaagag gagactctgc tgcgggcgga aagcgcgcgc cgccgggctc
    17161 gcgcgaacat cccggcggat gtgttcatcc acttcgacac gccgctgacg ctggagcacg
    17221 agcttgccct gctgcgggcc gcgggctttt cgtgctgcga ggccgtggac agcatcgcgg
    17281 gagccacgtt cctgcgcggc cgcaaataag actggtaaca gaaaagcctt acaaaagagt
    17341 aagaaagctg taagccctgt tccggttgcg cgcggcgcac agccatgata caataatata
    17401 tccttatttt tgctgcaaag gaggccgcgc ccagtgcagt tcacatccct tgcctatctc
    17461 ggctttttcg ccgccgtgtt cgccgtacac tgggccgtgc cgcagaaagc gcgctgggcc
    17521 gtttgcctgg ccgcgtcggc cggtttttac gcgttgctgg gcgcgcccat gcttgccgtg
    17581 ctggcgctgt gcatcgccgt ctcttatctg ggcggcctgt ggctgcagcg gcggcggggn
    17641 nnnnnnnnnc ggcggggccg caggcccgcg ctggcgctgg ccgcggcggc ggcgctggcg
    17701 ccgctgctgt tcttcaaata tttcaatacg ctgctgggcg cgtatgtacc cgaaagcgtg
    17761 cgcgcgttca gcctgctcac gcccgtcggc atcagttttt tcacgttcaa agctgtggcg
    17821 tatctggccg aggtgtacaa gggcgctctg cctccctgcc gccatttcgg acggtacgcg
    17881 ctgtatgttt cattctttcc cgaagtgagt atgggaccca tccagcgccc gggcgacctg
    17941 atggcccaga tcggcacgcc ccgcgcattt gacgccgggc gcgcggtacg cggcgcgcag
    18001 ctcatgctgt gggggtattt tgaaaagctg gtggtggccg acaacctggc gccttatgtt
    18061 tccgccgggc tggacgcccc cgaccttgtg atgggccttt cggtgttcct ggccagcgta
    18121 ctgtacgcgg tgcagctcta tgcggatttc gccgggtatt cccacatcgc catcggctgt
    18181 atgcagcttt tgggctttga cgtaccggaa aatttccgcg cgccctattt ttccacgtcc
    18241 gtgaaggagt tctggaaccg ctggcatatt tcgctctcct cctggctgcg ggactatgtc
    18301 tacatcccgc tgggcggcag ccgctgctcc acagcgcgct gctgcgtcaa tctgctgata
    18361 accttccttg tcagcggcct gtggcacggc accgggcttc aattcgccgt atgggggctg
    18421 ctgcacgggg cataccttgc ggtgggacgg ctcaccgcgc ccgcccgcgc gcggctgtgg
    18481 cgcgcctcgc acattccgga gcagagcgcc ccggcccgcg ggctgaaaat gcttgtcact
    18541 ttcgtgctgg tgtggttcgg gtgggtgttt ttccgcgcgc ccaccctgtc cggtgcgctg
    18601 catctgttcg cccgcatgcc ggacagcttt tctcttacgc ccccggcgct gaaaaacgct
    18661 ctcgtcatgc tggggtttaa cagcggtatg ctggtacgcg tcggctttgc ctgcgctctg
    18721 ctgtgcgcgg tggacttcgc cgcgaggcgc acgggctttt ccgcctggct tgcggggcgc
    18781 agaaaatggt tccaggccgc gctgtgctat gtgctggttc tggccatcct gtttcttgcg
    18841 cccatcggcg catcccccac gcccatctac ttccagtttt aagcgccgcg cccccgaacg
    18901 gacgcggccc gaatatccgg gaggcttcca ttgaaacgct ttttaaagat cctcgcgctg
    18961 tttctcgcgc ccgtcatcct gctgctgggc atgttttccc ttgcccttgt gcgctcgggc
    19021 gagctgacgc ccacagcgga cattgaagcc gctgcgctgg acggcgggct ggagctgttc
    19081 ggccttgcct accgggatga tacccgcgcc ttgaagcagg ccgtggccaa cgcccgcggt
    19141 gcggacgtgc tggtgctggg cacctcgcgc tccatgcagc tgcgcggcgc tttcttcgca
    19201 tcagacagct tttacaacgc aggcggcggc atcgcctata tttcacaggc ccaggtgttt
    19261 ttggaaaaca tgccgccgga cgcacggccg aagcatctgc ttctggtgct ggaccagtat
    19321 ttctataatg agacctggac ctccatcgaa ccggaggaca gcgcggccct gcggccctat
    19381 acgcagccgg acgcctttta cgctctgcgc cgggcactgg cggattatct ggacggaaaa
    19441 tattccctgc tgcatgtgct gggcacgcag gacggcgtgt acggcatgtc ggccgccggg
    19501 cgcggcgcag gcttttacgc agacggctca tacacctacg gcaccgccgt gctgcacccg
    19561 gaaaaaagcg tggacgcaga gtttaaggat acgttccagc gcatcgcaaa aaacacgaac
    19621 cgctttgagt acggcgaaac gccggacgcc gagagccttg cccaaacgga agctctgctc
    19681 gctttctgcg cccgggccgg catcgaggta acggcattcc tgccccccta cgccccctct
    19741 gtgtggcagc gcatgcagga gaccgggcag tacggctaca tcccggccac cttcgcgtcg
    19801 ctggagacga tgtttgcccg gtacggcttt gaggtgttcg attactccta tctgccggag
    19861 acgaatgaca gccagtatgt ggacggcttc cacggctccg accgtgtata tgcggcgctg
    19921 tgcgcgcggc tggcggagga ttcgcttctg ctgggcgcac agttcgacag cgccgcgctg
    19981 acggcgctgt ttacggcgca gggaaaccct ctgacggttt ccctgccata gacccgcctt
    20041 aaaaagcaga aacagcggcg ggccgcccac accgggcgcg ccctccgcnn nnnnnnnncc
    20101 tccgctgttt ttggtatttg atcccgtttt ttcagccgcc gatcaggaag tccagaacat
    20161 ccatcgctcc gtcgctcatt tccttgtaca gctcggtata gccgcaccgg gtgcaggtga
    20221 tggtggtgaa gcgcttgttc tgtacgtcga acagcttggc aaaattgccg ccggttgcct
    20281 ggaactggtc ggcctcatac tccatatttc cgcacttcgg gcatacatac tgctgccgtt
    20341 ccatggtgca tcccctcctt ttggtaccag tttacgcgag attaagccgc tttgtcaaca
    20401 accagcgcgt tatgcagaaa taaatcacac agccgaccag cagcactgtg cccacggccc
    20461 ccattacggc gcacatcacg gcggcgggtg cggcgtgctc taccacaggg accagcgcat
    20521 ccatagcccc ggttgccaca agcaccacgc cgctgacgag cataccggcc accgtcagca
    20581 tctgcagccc aaagttgagc acaagatacg ccgcgatgct ggctcccagg cggctggtgg
    20641 gccactgccc gccgatggcc atgcacatgt aaagctgtaa atatgtgttc accgtaccga
    20701 taataaaaaa caccagcatc acggcggcgg gcatccatag gcccgtcccc atctccgcgc
    20761 ggaacgccgc gctgaagcgt gcaagcgtca tgccgttttc cgccgcgccg ggaatagaga
    20821 agatcaatgc agcggcagcc agcaccgcca gcgccagtga aaggagcgtc cacactgtgc
    20881 ccacggccag cttggccgtg atgtgctggg cgggtgtgac aggcagcgtg aacatcagat
    20941 atccctcgtc ccccagcagg tttttgtaaa accgctggat ggtgacgacc accgcggccg
    21001 tcatcatggc catcattgca ataaaggtga gcaggcacag cagcccggaa gcgaggccga
    21061 gcaccggccc tccccagccc gttccgccgg gcagcaccag tatgccgccc agctttacgg
    21121 tcagcgccgc cgcgcccgtc agcaccagaa ccgtgcccag tatgggcaga aaaatacgcg
    21181 ccgtcgcctt gaattcatgt ttgatgagtt tcgctaacat ttgaatacct ccctgaaaaa
    21241 cgcgtcgacg cttttgccct gcgccgcacg gagctcatcc acactgccgg acgccacaag
    21301 ccttccgccg ccgatcatca cgacgtcgtc cagcaccttt tcaatgtcgc cgataagatg
    21361 tgtggaaagc accagcgccg cgtcctcgct ataattacgg atgattgtgt ccagaatgta
    21421 atccctggcn nnnnnnnnng gagaaggtgc agcttgtgct ggtgatgagc cgcgccgccg
    21481 atgggctcat ccagcagata caacccggcg gcgcggctca tcaccagcac aagctgcacc
    21541 ttctcccgcg tgcccttgct catggcgctg atcttgtccc ccggctggat cttcagatcc
    21601 gccagcatcg agaccgcctt ttcccggctg aaatcagcgt aaaaatcgca gaacagctcc
    21661 agcgcctcgc gcacgcgcaa accggcgggc agatacgtgc gttccggcag atacgccacg
    21721 cgcgctttcg tctgcgggcc cggcgcctgt ccgcatacgc gaaggcttcc gcccgtgggc
    21781 accagcagcc ccgccgccag cttcatcagc gtcgtcttgc cgctgccgtt cggccccagc
    21841 aggcctgtga tgcgcccgcc ggaaaaagcc gccgtaaaat tgtccagcgc cttgttcggg
    21901 ccataggcct ttgtcaatgc gctgcatgtc aataccgctt ccatcgtcat tcctcctgtg
    21961 ccttttgctg caaaagcgcc agcgtctgcg cccgggtgta tcccagcttt tccatcatct
    22021 gcaaaaatgc gccgatctgc tcggcagcca gttgttcgcg caattgtgcg atcattattt
    22081 catcctccgt tacggtgcgg cctgccgtgc gctgtgtatt caaaaggccg cgtgcttcca
    22141 gttcctgcag cgcacgctgc atggtgtttg ggtttaccgc cgctccggca gccatctccc
    22201 gcacgccggg cagccgtgcg cccgcccggt attcaccggc cacgatggca agctccagct
    22261 gttcgatgag ctgcaggtaa acgggccggt cggctgtaat gttccacttc atgcgctcgt
    22321 ctccttgtgt cactgtatta accacttaat acaataatac aatatatttt tcaaaagtca
    22381 atagaggaaa atataaaatt ggggctggac ttttcaaaga aggtatgcta taataatttt
    22441 cgctgtggag gcgtagctca gctggttaga gcgttcggtt cacatccgag aggtcgcggg
    22501 ttcgagcccc tccgtctcca ccatataaaa agccgcatta cactgtcatt tgttcggtgt
    22561 ttgcggtttt ctttttgcct tgtttatgcg cgccaatatc cttacggtgt tatttacaaa
    22621 atatgcaaat caaaatgcaa gccaaacata aattaaaact ccctcggtag ttgttaccgg
    22681 gggagttctg ttttatcaac agatatcaac gccgacagac tttgcctttt tatgggattt
    22741 gaggaagtgc caggagttgg actttatcct atattacata tccctttgca gccagcgctt
    22801 cggtatatta tgctccagtc tgatgattgt cttaaagcca tgagcggaaa atactgccga
    22861 catttgttct ccggtctcct ggggtacata aattacgctg caaa
//
LOCUS       DAWBSB010000007        20757 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_696_length_20757_cov_3.450874, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000007 DAWBSB010000000
VERSION     DAWBSB010000007.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 20757)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 20757)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..20757
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_696_length_20757_cov_3.450874"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4721..4739
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8270..8279
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3929 a         6147 c         6848 g         3804 t
ORIGIN      
        1 atgggcgcat agcccaaaag accgccgaag tccagcacgt cgccctcctt gcagccgatg
       61 gccgggatga cgcgcacggc cgtcgttttg gtgttcacca tgccgatggc ggcctcgtcc
      121 gcgatgagcg ccgagatgac ggcggcgtcc gtctcacccg ggatgacgac catgtcgatg
      181 cccacgctgc acacggccgt catggcttcc agcttttcaa tggacaggct gccgcgctcc
      241 acggctttga tcatgccgtc gtcctcggac acggggatga acgcgccgga aagcccgccc
      301 acgtggttcg aggccatcac gccgcccttt tttaccgcgt cgttcagcat ggccagcgtg
      361 gccgtcgtgc cgcagcagcc gcagctctcc agcccaatct cctccagaat attcgccacg
      421 gaatcgccgg gcgcgggcgt gggcgcaagt gaaaggtcca cgatgccgaa gggcacgccc
      481 agccgctcgc tggcaaggtt gcccacaagc tggcccatgc gtgtgatttt gaatgccgtg
      541 cgtttgatca gctcggccac ggtgtccagc gaagcgtcct tgggcagctg ggccagcgcc
      601 gcacgcaccg cgcccgggcc ggagacgccc acgtggataa cgcagtccgg ctcgcccggg
      661 ccgtggaacg cgcccgccat gaaggggttg tcctccggcg cgttgcagaa tacgacaagc
      721 tttgccgcgc ccgcgcacat tttgtcctgc gtgcgcgccg cggcttcctt gatgacgggc
      781 cccatcatct tgacggcgtc catgttgatg ccgctcttgg tggagccgac gttgatgctg
      841 ctgcagacgt agtccgtaca ggccagcgcc tccgggatgc tgcggatgag ccgctcgtcc
      901 ccggcggaaa agcccttgtg caccagcgcc gaatagccgc cgatgaagtt gacgcccacg
      961 gccttggccg cagcgtccag cgttttggca taccacaccg ggtccgcgtc cggcgcgctg
     1021 gccagcagca tggcgatggg cgttaccgag atgcgcttgt ttacgatggg catgccgtag
     1081 gtggctgaga tgtcctcggc tgttttgaca aggtcctttg ctttttcggt gatcttgcgg
     1141 tagatctttt cgcaggcttt tcggccgtcc gggtccgcac agtccagcag ggagatgccc
     1201 atggtcacgg tgcgcacgtc cagatgctcg tccgtgatca tctggatggt ctcgagaata
     1261 tcgttcgtgt tcaacatatg cgctccatct ccttaaatac ggtgcatggc gtcatagatt
     1321 tcctgccggg tgcagcgcac atccacgccc agctcgtcgc ccttctgctt catcttctct
     1381 gcaaaggccg taaaatccat cgagcagctg gacacatcca ccagcatgat catggcgaag
     1441 gtgccgcgca ggatgcgctg cgtcacttcc tccacgttta cattggcctc ggcgcacgcg
     1501 gtgcatacct gtgcaagaat gcctactttg tcgttgccca cgacagtgat aacagcttcc
     1561 attttattcc ctctcctccg ttgtgcccgc gccgtcgggg cgcggcgtcc gcaggcgggc
     1621 ccgtccggcc cgcggtctgt tttaccagta taacaaaaaa atccgccggg gaaaagagcc
     1681 gaaaccgccg aaatcccggc tccgtgcgcg gtgtgttttg tgcggatgtt tactttcggc
     1741 gccggattta ctataatgta cctaatatcg cctaataaat atcgcctcat aaatgaagag
     1801 gcgagatgta cagcgaagaa agaggggaac atccatggaa aagatactca agatcctgga
     1861 ggaaaacgcc cgtatcagcc ttgaggatat cgccacgatg gtggagagca cgcccgagga
     1921 ggtggcgaag cagatcgacg cgtaccagca ggcccacgtc atcaacggct ataaggcgct
     1981 gatcgactgg gacaaggccg gagccgaccg ggtgcaggcc atcatcgagc tgcgcgtttc
     2041 gcccaagcgg gactgcggct ttgacgaggt tgcggccaac atcgcccgct ttgacgaggt
     2101 ggacagcgtg atgctgatga gcggcgggta cgacctttcg ctggtgatga gcggcaagag
     2161 ctttcaggac attgcattgt tcgtggcaaa gcggctgagc ccgctggacg acgtgctgag
     2221 cacggccacg catttcgtgc tgcgcaccta taaaaaagac ggcgtgctgt acggcctgga
     2281 acccaccgac gaacgggagc tgctgtgatg gattatgcaa agatcatttc cgccgccgcg
     2341 cgcgacatgc gtccctcggg catccgcaaa ttttttgaca ttgcggccga gatgaaggac
     2401 tgcatctcgc tgggcgtggg cgagccggat tttaaaacgc cctggacggt gcgcgacgcg
     2461 ggcatccgca gcctggaaaa agggcgcacc tggtacacga gcaattcggg cctggcggag
     2521 ctgcgggagg agacctgcaa ttacctttcc cgccgcatgg gcctttccta cacgcccggg
     2581 gaaaccgtcg ttaccgtggg cggctccgag gccatcgata tgtgcatccg cgcgctggtg
     2641 aaccccggcg acgaggtcat catcccggag ccgtgctttg tctgctatta ccccatcacc
     2701 acgctgacgg gcggcgtgcc cgtgccgctg ccgctgaaag ctgaaaacgg ctttaagctg
     2761 acggcgcagg agctggaggc ggccatcacg ccgcgcacaa agctcatcat cttcccgttc
     2821 ccgtgcaatc ccacgggggc cgtgatgcgc cgggaggacc tggaacccat cgcggcgctg
     2881 ctgcgggata aggacatcat cgttctgtcg gatgagatct atgccgagct gacctacggc
     2941 ggcgagcgcc atgtgagcat cgcctcgctg ccgggcatga aggaacgcac cgttgtagtg
     3001 aacggttttt ccaaggccta tgccatgacg ggctggcgtc tgggctatgc ctgcgggccg
     3061 gagccgatca tcaaggtaat gacgaagatc caccagtccg ccatcatgtg cgcgcccacc
     3121 accagccagt acgcggccgt gacggctctg cgccgctgcg acgcggatat cgaggccatg
     3181 cgcagcgagt atgacatgcg ccgccgcttc ctcgtgagca gcctgaaccg catgggcctg
     3241 gactgcttcg agccggaggg ggcgttttat gcctttccct ccattcggaa aacgggcctt
     3301 tccagcgagg aattctgcga aaagctgctg ttcggcaaaa aggtggccgt ggtgccgggc
     3361 aacgccttcg gcgattccgg cgaagggcat gtgcgcatca gctattcgta ttccatcggc
     3421 catttgtccg aggcgctgca ccgcatcgag ctgtttttgc aggagctggg cgtgtaagcg
     3481 atggggaagg aagtacattg ttattccgtg ccggactatg cgtccgcggc gctggccgcg
     3541 gccgtggagg acatttttgc ccaaagcgcg gccgcccggg cgctgggccc gcgcagccgc
     3601 gtgcttgtga agccgaacct ggtggcgaaa cacgcgcccg aaaaggccgt caccacccac
     3661 cccgccgtgc tggacgccgt gtgtgcggcg ctgcggcggc gtggcgttga aagtatcacc
     3721 gtggcggatt cccccggcgg gctgtacaac cccgctgtga tgaagagcat ctataaggcc
     3781 agcggcctgc tggcggtgtg cgagcgccac ggcgcgctgg cctacacggc gtgcgaggca
     3841 gcgccgcgta aaacggatgg cgtgcgggtg aaggagttca gcctgctgcg ccccgttctg
     3901 gaggcggatt tcatcatcga cctgcccaag gtcaagaccc atgtgatgac gggcatgtcc
     3961 tgcgcggtga aaaatctgtt cggcacggtg ccgggcctgc agaaagccga attccatatg
     4021 cgcttcccgg aaaaggagcc gtttggagaa atgctggtgg acctgtgcga gaccgtgcgg
     4081 ccgcagctta tcattgccga cggcgtgctg gctatggagg gcgacggccc cgcgggcggc
     4141 acgccccggg cgctggggct ggtgctgggc ggagaggacg cgtatgcggt ggaccttgct
     4201 gtgtgccgcc tgatggggat gcctgcgatg cgggtgccgt accttgccgc cgcccatgcc
     4261 cgcggcttgt gcgaggcggc actggaggaa gcgctgctgc gcggcgacac gcaggcggca
     4321 gcgcccgtag cggactataa actgcccgcg ggctttgcag acatcagctt tgccgacaag
     4381 gcgccccggg cgctgcgctg ggccgttccg ggcgtggaaa agtggcttgc cccgcgcccg
     4441 aagatccacc ggagcaaatg catcggctgc ggcaagtgcg ccgagatatg cccagggcac
     4501 accatcacag tggaaaacgg aaaggcgcat atcgtgccga gggcctgcat ccgctgtttt
     4561 tgctgccacg agatgtgccc cgcaaaggcc atcgatgtga agcgggctgg gatattcaat
     4621 ctgtagacgg aaagaggcag aaatgaaaaa aggaatttca acagctgttt tgctttcggc
     4681 gctcctgctg ctcacgctgg cggccgtatt tctgctgcag nnnnnnnnnn nnnnnnnnnt
     4741 aaagggaggt cgttttcatg cgcagcgtca ccgtgcttgc acccgcaaag ctgaatctca
     4801 cgctggacgt gacggggacc cggccggacg gatatcacac gctggatatg atcatgcagg
     4861 ctgtcagcct gcgggagcgc gtcactttgc gcagaagcag gggactttcg ctttcgctgc
     4921 ccggctcccg cgtgccggcc aacgagcaca acaccgccta taaagcggcg ctggcgtttt
     4981 tccacggcac gggcctcctg gcgggcgcgg ccatcacggt ggaaaagcat gtgcccgtgc
     5041 gcgcgggcat ggcgggcggc agcgccgacg ccgccgccgt tctggtgggg ctgaacgagc
     5101 tgtacggcgc gcgcctttcc gcggcagagc tgtgcgcgct gggcgcgcag gtgggcgcgg
     5161 acgtgccgtt ttccattgtg ggcggcacgg cccgggttac cggtgtgggc gatatcctgg
     5221 agccgctgaa gccctgcccg ccctgctttt ttacggtatg tatgcccgcg ggcggcattt
     5281 ccacgccgca ggcatatgca aggtacgacc gcattggtac cgacgtgcgt ccggacagcg
     5341 cggctgcggc ggccgccatc gcacggggcg atttggcggg cctgtgcgcg cagatgaaga
     5401 acgcgctgga atattccagc gccagcgccg cgacaaagcc catctgcgaa acgctgcgcg
     5461 cccacggcgc gttggcagcg ctgatgacgg gcagtggggc ggcggtgttc ggcgtgtttg
     5521 cagacgaggc cccggcccag gccgcgaaag cggcgctgct gcgggtttac ccacagtgct
     5581 gggtgctgcg gccggaggcg aaaggtgcgc gcgtcgtggg gcgcggatga ggattaaacg
     5641 ctgtgggacc gaaaaacagg aggcgctgtg ccatggaaac agaacgcaag ctggatgtat
     5701 tggccgatat tgcgcgggcg ctgaaccaaa gcggcgtcac ctgggccgtg ggcggttccc
     5761 tgctgctgta ttttaagggg aaaacgggcg tgttccgcga cattgacctg atggtctgtg
     5821 aaacggatgt ggaaaagctg aagcaggcga tgctgccgct gggcgcgccg gccccgtccg
     5881 acccgaacgc acagtatagg acgcgctgct tcctggaatt caccatccgc ggcgtggatg
     5941 tggacgtgat ggcggggttc gtcatcttgg aaaacggaca ggaacacgac tgcgcgctga
     6001 agccggagca ggtggcggag catatccttg tacgcggcga agatatcccg ctgcaatctc
     6061 tggaggactg gcgcagatat tacgcatgga tgggccggac atccaaggtg gcgatgatag
     6121 acggaaaaac aatgcggtga gagcggagca gcggccccgt ccggcagtgc gccggacggg
     6181 gccgctttgc ggcggacgcc ggaccgtgcc caaaggggtt gcaaatccgg gccgggcatc
     6241 gtacaataga gtggcgcggg gcgctgtgct gccgcgcaaa ttgaaacgac aaaggaggaa
     6301 gcgtctttgg aacccgttgg acgaagtatg ggattgcccg gcagacaacc ggatcagaca
     6361 ccaccggcgg ccgcagggcg cttcgggcct gttttctgac gctctttttc ggggcgcaga
     6421 gcgggctgtg cgcgctgcac gcccgcattt gcgtgttttg gttttgtttg ccgcggcggc
     6481 gggcctctgg tgcctgcttc cgcggctttt ttgtccgggc agacaccccg tgttcgtcct
     6541 ccgacacaca ggaagaagga ggaaacacaa atggatcggc agtatgattt tgcagcgctg
     6601 gaggcgctgc tgaaaaaacg ttcctttaag gctctgcgcg aggccctgct ggagctgaac
     6661 gaagtggata ttgcagaatt tctgggactg ctgaacggtg aggaggccgt gctggctttc
     6721 cgcacgcttc ccaagggaat ggcggccgac gtgttcgcag agctggagcc ggagatgcag
     6781 cagctcatcg tcgctgcggc gacggatgcg gaattggccg tcatcgtgga ggacctgtac
     6841 gtggacgacg cggtggatat gctggaggac atgcccgcca atgtggtcaa gcgcatcctg
     6901 aagaacgcca caccggagat gcgcggtctg atcaaccagt tcctcaagta tcccgagcat
     6961 tccgccggca gcattatgac ggcggaattt acagatctgc gccccacgat gacggtggag
     7021 caggccattg agcacatccg ccgcaccgga gaggaccggg agaccgtcta cacctgctat
     7081 gtggttggcc cccgccgcag gctgctgggc gtggtgacgg tgcgcagcct gctgctggcg
     7141 cgggacagcc agctcgtcag cgatgtgatg gaggacggcg tcatctccgt cgccaccggg
     7201 acggaccagg aggaggccgt gcggctgctg cagcggtacg gcttcctgtc gctgcccgtg
     7261 gtagacgcgg aaggacgtct ggtgggcatc gttaccgtag acgacgcggt ggacgttatg
     7321 gagcaggagg ccaccgagga cttcgagaag atggccgcca tggccccatc ggagaagccg
     7381 tatctgaaaa cgggcgtgtt ttccctggca aggcaccgca tcgtatggct gctggtgctg
     7441 atgatctccg gcatgatcac aggcggtatc ctgggtcggt atgaggcggc cattgccgcg
     7501 atgccgctgc tcgtcacgtt tattcccatg cttacggata ccggcggcaa cgcgggcagc
     7561 cagagcagca cgctggtcat ccgcggcctt gcggtgaacg agattaccct gcgggatttt
     7621 ccccgggtgc tgtggaagga gctgcgtgtc agcctgctgg tgggcgtggt gctcagcgcg
     7681 gtgaattttg ttcggctgat cctcacctat cccggcagcg agatggtggc cctcactgtg
     7741 gcgctgacga tgctgtgcac cgtgatcgtg gcaaaaacca tcggcggcgt gctgccgctc
     7801 attgcaaagg cctgccggat ggacccggcc atcatggcct cgccgctcat caccaccatt
     7861 gtggacgcgg tgtcgcttgt gatctatttt gccgtggcgc accggctgct gccgctgtga
     7921 cgcgccggca aagccgccgc cccgtgtccg ggagcggcgg ctttttcatg ccggcaggca
     7981 aaaccgccgt aaaaatgtga aatctttgtt ttgcgggaat gctgggaata attggcgtaa
     8041 ggtctgtcca cacttcggcc atagggagtg tgaataccga tgagattact taaaaaatgc
     8101 aagacactgc gcgcggcgct gggcggacgc cttgcccgca tggggcgccg gggccggtgg
     8161 gagctttccg tcctgctggg gctggcgctg gcgctgagcc tgagcagctt tacccggttt
     8221 tccgccgcat gtgcgggtgt acgggaggat acgctgcgct tgcatatatn nnnnnnnnnc
     8281 gccttgcact gtgcctgggc acgctggcat gcattctgct ttctcaattt gccgcgtttg
     8341 cgcaggcgtg cgccggtgtg cggaaggata cgctgcgcct gcatatattg gccaactccg
     8401 attcagaggc ggaccaggaa ctgaagctgg cggtgcggga tgcgatcctt caggcggagg
     8461 gcggcatgtt cgctgccgaa cggacaaagg aaggggcgct gcgcgccgcc ggggcggacc
     8521 tggaggccat ccgcgctgtg gcccagcgca cggtgcgtgc ccgcggatac agctaccctg
     8581 tagcggttcg actggagaat atgtattttt ccactcggga atatgacggc ttcacgctgc
     8641 ccgccgggcg ctacgacgcc gtgcgggtgg agatcggcgc gcatgccggg aaaaactggt
     8701 tctgcgtgct gtttccgccc atgtgcgttc ccgcggcaac atccgacggc gggcaggaca
     8761 tgtcggctta ttccggtgcg gagcagcagg ctgtgtgcag ttcttataag gtcggctttg
     8821 cggctgtgga atggctggag gggctgaagg aaagcctgtc cggccagggg gtgcggacgc
     8881 tggccgaggc ggacgggcag gcggaacagg acgacgaagc acaagaggag ccggacgggg
     8941 cggaacagga cgcgcgacgg gcggacaaaa aagagaccgc ctctcgcgcc gggacggagc
     9001 attccggcgg gcggacggtc acaacggcag atcgcacaca ggcgcaagct gcacgtacag
     9061 cggactgaag cggtcggcca tatagtcggg cacgctgggg gcggcaccgg gcgggcacag
     9121 cggcaggcac agggaaaagc acgcgccgcg ctccggcgcg ctgcctgcca gcaggcggcc
     9181 gccgtaggcg cgggcatagc gccgtgcaac ctcaagccca aggcccaggc cgcggtcccc
     9241 ggcggagggc gcgcatcccg ggccgttgtc ggccacagtg agaagcagcg tttccccgcg
     9301 ccgcgccgca cgcacggata ttgcgggccg ctcgccgcca tactgcaccg cgttggccag
     9361 caaattaccc agtgcggcgg caaacagccg cgcatctccc atggcccaca gaggcgcccg
     9421 gggccattcg ggcacgaaca ccgcgctgcg gcacacaccc gccgcgccgc tgacgtaggc
     9481 cgcggccagc gctgtaacgc acaccggctg cgggtggggc ggtgcggtgc agagcgcggc
     9541 gtgagcggcc aggttttcgg cggtgcgcag cgtgcggtag gccgcgcggg cgatgccgtc
     9601 cagcgtggct tcgccgccgc cggggccgcc cgccgccgca cggcgggccg cgggcagcag
     9661 ggccagaagg tcggctgtat cctcacgcag atgcgccgca agaagcgcgc tgtttggcgt
     9721 cggggccatg ggaatcctcc tttccgcatg cgcgccgcag ggagttggag gcggcgcgca
     9781 gggtcaaact gcacatagtg tatgaagttt gcacgccgct atgtatggat tgttacaaaa
     9841 gtgtaagaag ttcgtgtttt cggcgttaaa cttttatcaa actcttgcag tccggcggct
     9901 tttggggtac aataaatgat agtcttacgg gcaaaataca gcgccgcagg gtgcggcgca
     9961 caccgtgcct gaatataagg aggatgtact aatatggcag taaaagttgc aatcaatggc
    10021 tttggccgca ttggccgcct ggcgttccgc cagatgttcg gcgccgaggg ttacgagatc
    10081 gtcgcgatca acgacctcac cagcccgaaa atgcttgccc acctgctgaa atatgacacc
    10141 gcgcagggcc gttacgaggg ccacaccgtc gagtccgacg agacttccat caccgtggat
    10201 ggcaagaaga tcgagatcct ggccgagaaa gacccggcga acctcccctg gggcaagatc
    10261 ggcgttgacg ttgtgctgga gtgcaccggc ttcttcgctt ccaaggccaa atcccaggcc
    10321 cacatcgacg cgggcgccaa gaaggtcgtc atttccgctc cggccggcaa cgacctgccc
    10381 accatcgttt acagcgtgaa tgagaacacc ctgacggctg aggacaccat catctccgcc
    10441 gcttcctgca ccaccaactg cctggcgccg atggccaagg ccctcaatga cgcctatccc
    10501 atccagagcg gcatcatgac gaccgttcac gcctacactg gtgaccagat gatcctggac
    10561 ggcccgcacc gcaagggcga tttgcgccgc gcccgcgccg gcgccgccaa catcgtgccc
    10621 aacagcaccg gtgccgccaa ggccatcggc ctggtcatcc ccgagctgaa cggcaagctc
    10681 atcggctccg cccagcgcgt gcctgttccc accggctcca ccaccattct ggtggccgtc
    10741 gtcaagggca aggacgtaac gaaggagagc atcaacgccg ctatgaaggc agcctcctcc
    10801 gagagcttcg gctacaacac cgacgagatc gtctcctccg acgtcatcgg catccgctac
    10861 ggcagcctgt tcgacgccac gcagacgatg gtcaccaagc tcgacgacga tacctatcag
    10921 gtgcaggtcg tgtcttggta cgacaacgag aacagctaca ccagccagat ggtccgcacc
    10981 atcaagtatt tcgctgagct ggcgtaaccc atgttttccg gcgctcccgc ttctcaaacg
    11041 aggcgggggc gttttttgtt ctttgtatct gcagcattta cagctttttc acacaaaaca
    11101 cacactgccg cctaaaaagt ggcaggaatc acggttgaac aacggggaca aacagggtat
    11161 aatattttgt atattgggga acttttggta tccgtaaaaa tgagccgaaa aggagggcgc
    11221 acatggagca gcggcacatg caggaaaaga gccgcggcgc ggcgcgccct gatgtggacg
    11281 cccgtgcccg ggccattcag gagcagatca tccatatccc gcgtccggtg cagcccggca
    11341 atgcgcgtcc ggccgcccaa agcacggccg gacacccttc gggcgcacag cagcgtcctc
    11401 ctcaagcggc acgcccccgg gatgacgctt gcgcccagcg gcggcgcgca gcgcccgctc
    11461 cggcacacac agcgccccgc gggcaggctg cgggcggacg ccactatgcc gcaaagccgc
    11521 gcaaacgcag gcgcgcgcca ggcggcggta tgttctttgc ctgtgtggcc gttgttatcc
    11581 tgagcctttc gtgggtcatc accacgctca tagagctgcc cgccaagcgc gcggcggcca
    11641 gcagtttggc tgctttgtcg gcttctcagg ctgcttcctc tcaggcagac ggcagcgtgg
    11701 cgcagacagg ggaagcggtg cttggccctg tacagcaaac agacgcttcg tatacgcagc
    11761 cttccgcttc actggtggcg ctgccggagg cgggccgtgt ggatatgagc tactttgacg
    11821 atgcgctgtt catcggcgat tcgctgacgc ggggcttcca ggaatattcc agcggcatcc
    11881 ccaacgcgaa atacgccgct tatctgggcg cgggccccaa gcagttcatg gagggccttg
    11941 tggaaaacat cagcggccag caggtggccg ccatagatga gatcttggcc gcggcgccta
    12001 aaaaggtgta tattctgctg ggcaccaatt ccatggctac gcttaccgat gaggcattcc
    12061 tgaagtatta caacgatttc cttgacttcc tgctgccgca gctgccgcaa gatacggtgt
    12121 attacatcca gggcatcccg cctgtctcgg cggagaagat ggccggggac gaaaactttt
    12181 cagtggaacg catccggggg ctgaacgaaa gcctggcgaa aatagcttat gacagggatc
    12241 tgcattatct ggacctgttc tccgctctgg ccgacgaaaa cggcgcgctt cgggcggata
    12301 ttgcttcggg cagcatccat ctgaacaacg aaggctacaa tgtatggcgg gagttccttg
    12361 tgacacatac ggcgtacagc aaggagaatc cgtacctgcc cggctcacct tattacacgg
    12421 ccccggcggc ctgacgaaaa cacggcacac gcgcggacgg cggaaaacgc cctgtgggag
    12481 ggactggcct tggcagagag cacgttcaaa ctgtatagca aacgccgccg aagccgggcg
    12541 cttctggcac aggcgggcct tggcgtgctc attgtggcgc tgctgttcgg tatttcctat
    12601 gctgcggtaa ggatcattta cggccagccg ctgctgcgcg cgcgtgccgc cgtgtccgcc
    12661 gcgccgccgg cacagcccgg cgaggtcccc gcgccggggc ctgcactgga aagccagccg
    12721 catgcgccgt ccgtgacagc ggccgtatcc gtgcaggcgt ggaacaccga aacccctgtg
    12781 gagcgcacgc tggatgccga tgtgacggcc agtgacgccc gccttctggc cgtaccggaa
    12841 aacgggcgtg tatccgatga ctatttccgc gatgcgctgt tcatcgggga ttccgttaca
    12901 cagggcttcg catggtatcc ggaatacagg gactggatgc gtgtgtgcgc ctacaagggc
    12961 gtgaaccccc agagcatcct gcaaaactat gtgggccagc gggccgatgg caccaaaatc
    13021 gagatgtggg acgagatcaa cgtccagcag gatgtagcga acatctatat ccttctggga
    13081 gcgaacgcgc tgctgcagca gacggacgag ggctttttga aattttacgg cgaattgctg
    13141 gacaaattgc gggtgcgttt cccggatacg cccatctatg tgcagagcat cactccaacg
    13201 acacaggaat acggcgagaa gaaaccgcag ctggaacggg agcatctgaa gcttatcaac
    13261 aacgccatcg ccaagctggc cgtgagcaaa gggctttatt atgtggacct gtgggaagtg
    13321 atggcggacg agaacggtta cctgcgggcg gacctggcag ggagcgacgg cctgcacctg
    13381 ctcgacggct ccaaataccg cccgtggctg gattaccttg ccgcacacac catatacagc
    13441 gcgcacaacg cgcagttcgc cctggcggac gggggcgcat accgttagca cgcccgcaaa
    13501 tatcctttgg aggcaccaat ttggcaaaca atatcaagcc gtacaaagtg cacagcaaac
    13561 gccgccgcaa ccagacgctt ctggtgcagg cgggtcttgg cgtgctcatt gtggcgcttt
    13621 tgttcggcat ttcctattcg tcggtgaaga tcatccacgg ccagcctgtg ttcccggtgc
    13681 gcggcgcgca gcaggacggg ggcccgccat cgtccgaaag cacgccgcct gcgccggaaa
    13741 gcgtatctac gccctccggc ggcggtgcgc aggaggtggg cggccgaccg gaggcagcgg
    13801 cgcccgtgca ggcttggaac acggaagtgc ctgtggagcg cacgctggaa agcgacgtta
    13861 ctgccagcga ttcccgcctg ttggccgtgc ctgccaacgg caggctgagt acggagtatt
    13921 tccgcacggc gctgttcatc ggggattccc tgtcccaggg ctttgcgctg tatgcaccca
    13981 cgcgggatgt ggcaacagta tgcgcgtata agagcaccag ccccaatcag gtgctgcaaa
    14041 attttgtggg ccagcggccg gacggctcgc gcattgagat gtgggacgac atcaaggtgc
    14101 agtcgccaag caatatctat gtgctgtacg gcaccaacgc gctcatcagc cagtcggatg
    14161 aagcgttcct gaaatattac agcgatctgc tcgacaagct gcgggagcgg ttcgcgggcg
    14221 tgcccattta tgtgcagagc atcacgccca ccacggccga acagggtgta aagcagccgc
    14281 cgctggagaa cggccacatc cgtcttatca ataacgccat cgccaagctg gccgtgagca
    14341 agggccttta ttatctggac gcacaggagg ctctggcaga cgcagacgga aacctgcgcg
    14401 gggactatgt gggaacctac gacggcattc acatgaaccc cacgggctat gcggcctggg
    14461 cggattatat cctgactcac accgtataca gcgattacaa caagcagttc gtgacggagg
    14521 ggccgtatgc ctgacgcacc ggccccggtg aaagataaca gcagggcccc ggctgaaaag
    14581 ccggggccct gctgtatctg ctgtgagccg ctgtcaatcg tgcaaaaggt tgagcgtgat
    14641 ctcgtatttg ctgtccactg aatacttctc cggtgcgctg gccttgtatt tgttcagcgc
    14701 ggcagtggat ttcaccgggt tctgcagcgt gccggcgccc gccacgcagc cgcccgggca
    14761 ggccatgccc tccagcaggt agccgttcag cttgcctgcc ttggccagca tcagcatttt
    14821 tttgcattcc tgcaggccct gggcggccat cacctttacc tcgcggtccg ggtccatctt
    14881 gtggatggcg ttcaccaccg cgttcgccac gccgccggaa accgcaaacc cgcggccgtc
    14941 ggcggtggcc tccaccagct cgggcccggc ggccacttcg gagaacttga tggccttcgc
    15001 ctcaaacatg cccatcactt cttcaaatgt gagcacgaag tccacgtcgc tgcggatggt
    15061 gcggcggctg gcttccagct ttttggcggc gcacggcccg atgaaaacga ccttgcagtt
    15121 tttgtgctct tttttcagca ggcgggccgt cagcaccatg ggcgtcatgg ccatggagat
    15181 atagggcgca aaatccggga acagcttttt cgccatcacg ctccaggccg ggcagcagga
    15241 cgtggccatg aacggctgct gctcgggcac ctcgcgcata aaatccttgg cttcttcaat
    15301 ggtacacagg tccgcgccga tggcgacctc caccacatcg tcaaagccca ggagcttcat
    15361 ggatggcttc agcttttcag gtgtcatgtc cgggccgaac tggcccacaa aggccggagc
    15421 cacggcggcg atgacacggt cgccctggtt gatggactgg atgacctgga agatctggct
    15481 tttgtccgcg atggcgccga aaggacagtt tacaaggcac atgccgcagg acacgcactt
    15541 gtcgtaatcg atcttcgcgc ggcccagctc gtcggagccg atggcatcca tgccgcaggc
    15601 cttggcgcag gggcgttcca tcttatggat ggcgccgtag gggcatacgt tggcgcactg
    15661 cccgcacttg acgcacttgc tctggtcgat aacgcttttg ccgtgcacga tggacaccgc
    15721 gcccttggga catacttcca cgcagggatg tgccaggcag ccctggcaca tatccgtcac
    15781 gtgaaaagat ttttccgggc aggcgttgca ggcgaatttg atgatattga tgaggggcgg
    15841 ctcgtagtat ttctccggcc gggcggcctc ctcgataccg gtggaaaggg gcgcgtgctc
    15901 gtcgatgggg cgcagcggca aacccatgga caggcgcagg cgctcgccca cgatggcgcg
    15961 ctccagaaag atactgtcgc ggtatttgct cacctcgccc ggcaaaatct tgtacggcag
    16021 ctcctcgacg cggctgtaat cgccgccttc gtatgccata cgggccacct cggtgaatac
    16081 ctggcggcgg atgtcggtaa cggaagaata aataccacgc atgtgtgaaa actcctttat
    16141 caatcaggtg tggacagact attacaaaaa tatcatacca tagtgcaaaa agccgcgcaa
    16201 gtgcgcgccg tgacaaagtt ctttgctccg cacaaaaaaa tagtggtaca tctgcccagc
    16261 gccgggcgga tgcccctcgg gggcttcttg taaaggccgg gcgaagggca tataatacgg
    16321 gagtggtatg attttccggc gcggcatgcg ccggctgtga gaaaaggaga accctgtttt
    16381 gattttggaa cttttactgg acgcgctgct ggacagcgtg aaaatgctgc cctttctgtt
    16441 cgcggcgtac tggctgatcg aatatgtgga gcaccgccac tcggagggca tcgaaaaagc
    16501 actggcgggc ggcgggcgct ttggctttgt gccgggcgcc gtactgggcc tgctgcccca
    16561 gtgcgggttt tcggccatgg cggcaaacct gtacgccagc aaggtggtca cgctgggtac
    16621 cctgctggcg gtgttccttg ccacgtccga cgaggcggtg ccgctgctca tcgcaaaccc
    16681 ggaacactgg cggtcgctgg cgctgatcct cggcatcaag ctggctgtgg cgcttgccgc
    16741 gggcttctgc attgattttg tgttcaaaag gctgattcct cagagcgtgc gcggcggcta
    16801 cacaggcagc agcagcgctg tggactgcca cgagcacgag gaacgggacg gcatcctggc
    16861 ggcggcgctc aagcacacgc tgcacatctt tttgttcatt ttgctgttca acattgcttt
    16921 gggcgcgctg gtggctctgg tgggcgagga ggccataggg gcattcctgg cggccagcgg
    16981 cccgtggcag cctgtgctgg ccgggcttgt ggggctcatt cccaactgtg tgtcctcggt
    17041 cctcatcacc cagctgtatg ccgcgggcca gctttcgctg ggaggcgtgg tggcggggtt
    17101 gtgcacgggc gcaggcgtgg ggctggcggt gctgctgcgc gccaacaaaa acgccaaaca
    17161 gaatttgttt atcgtggcgc tgctgtacgg catcggcgtg gcggcgggcc tggtttgcag
    17221 cctgtttgca tagcaaaatc aaaaagcacc ccgccgcacg gtggggtgct ttttgcgcgg
    17281 tgcgggcaag ggacagggaa aggccgtgcg ccatacaatt tccgccaatt gccgaatgac
    17341 acggcggacc gtttgtggta taatatgtcc ggttggtgaa tgcgagccgc aggcgaagca
    17401 tgagcctggc ggcatagata ccatgtttct tagtgaacac gagcggtacg cgaagtgcgc
    17461 gatgggaaac atagtttgcc tggagcaacg gaaaatccgg cgcgcctcaa agcgcgtgcg
    17521 gcaaataaag gcgtgtcccc cggtgcgggc ggttttggtc cgctgtgccg ccggcgggcc
    17581 gctgcggagg gaaacgatat gctggatgtg gccgtcatcg gctgcggcat cgtgggcgca
    17641 aacgtggcct atttgctgtc gcagcacaag ctgcgcgcgg cggtgtttga aaaggagaac
    17701 gacgtctcca tgggcaccac acgcgccaac agcgccatcg tgcacgcggg gtacgaccct
    17761 gaaccgggca ccgccatggc gcggctgaat gtgcggggaa gtgcgctgac gaaagagcag
    17821 tgcgctgcgt tcgacgtcaa ataccgccag atcggcagcc tggtgctggc tttttccgat
    17881 gaggaaatgg caaccgtgcg ccgcctgtac gaaaacggtg tggcgaacgg cgtgcccggc
    17941 gtgcgcgtgc tgacgcggga ggaaacgctg gccatggagc cgaatctttc cgatgcggtg
    18001 tgcggcgcgc tgtatgcgcc cagtgcggcc attgtggagc cgtgggatct ggcgctggcg
    18061 ctggccgagg ccgccgtaga aaacggcgtg gaactccatt tggaaacagc tgtgacaggc
    18121 gcggagaaaa tcagcggcgg ctggcggctg catactgcaa aaggcgattt tgaagcccgt
    18181 gccgtggtga acgcggcggg cgtggaggcg gacctggtgg ccgcgatggc gggtgaaccg
    18241 ggctttacca cgattcccag ccggggtgaa tactaccttc tggacaaaag ccagggctat
    18301 gtggtgaacc acgttgtttt ccagtgtccc aaccgcgacg gcaagggtgt gctcgtttcg
    18361 cctacggttc actacaacct tatcgtcggc cccaatgcgg agcccagcgg ccgcgaggac
    18421 gtttccacac aggcggacgc gctggcattc gtgcgggaaa aggccgtcaa gagcgtgccg
    18481 ggcgtaaact tccgggaaaa tatccgcaat tttgcgggcg tgcgcgccaa taccgaccaa
    18541 agcgatttta tcattgggga gaccgcgccc ggcttcatca cgctgggcgg catcaaaagc
    18601 ccgggccttt ccagcgcgcc ggcgattgcg gaggacgcgc tggcgctggt tgcgggcgcg
    18661 ggcgtgacgc tggagaaaaa ggagagcttc gtccacaccc gcaccaaaaa acgattcaac
    18721 gaaatgacgc cgcagcagaa ggcggaggcc atccgggaaa atccgctgta cggccgtgtc
    18781 atctgccggt gcgaaaccgt gacggagggc gagatcgtgg acgcgctgca ccgccccatc
    18841 ccgccgcgca ccatcgacgg cgtaaaacgc cgctgcaccg ctggcatggg ccgctgccag
    18901 ggcggcttct gcggcccccg ggtgcatgag atcattgcgc gggagcttgg catcccccag
    18961 caggacgtcc tgaaggacag ggccggcagc tatatcatca ccggcaccac gaaggaaggg
    19021 ggcctgcatc atggagaata accggtacga cgtcatcgtc atcggcgcag gccccgccgg
    19081 gctggcggcg gccgaggccg cgcacaaggg cggcgcgggc cgcatcctgc tcctggagcg
    19141 cgacaacacg ctgggcggca tcctcaacca gtgcatccac aacggcttcg gcctgcatta
    19201 ttttaaagaa gagctcacgg gccccgagta cgcgggccgc ttcatcgagc tggtgcgcaa
    19261 gacgaacata gaggtaaaga cggacacgat ggtgctggcc gtcacgccgg aaaaacgcgt
    19321 ccacgccgtc aatgaaaagg acggctacat tgtgctggag gccgcgtcca tcgtgctgtg
    19381 catgggctgc cgcgaacgca cgcgcggcgc catttccatc ccgggtgcac gcccggctgg
    19441 cgtattcacc gcgggcgcgg cccagcggta tctgaacatt gagggctata tggtgggccg
    19501 ccgcgtggtg attttgggct cgggcgatat cggccttatc atggcccgcc gcatgacgct
    19561 ggagggcgcg aaggtgctgg cctgcgttga ggtgatgcct tattccggcg gtttgacgcg
    19621 caacatcgtg cagtgcctgc acgattataa tatcccgctg tatttgtccc acacggtgac
    19681 gaagatcgag ggggagggcc gcctgacggg cgtgaccgtg cagaaggtgg atgaaaaccg
    19741 cgctcccgtc cccggcacgg agatgcattt cgactgcgac acgctgcttt tgagcgtggg
    19801 gctcattccg gaaaacgagc tttcgggcgg cgcgggcgtg gagatggacc ggcgccacaa
    19861 cggccccatg gtgtatgaaa acatggagac cagtgtgccg ggcatcttcg cggcgggcaa
    19921 cgtggtgcat gtacatgacc ttgtggattt tgtcacgggc gagagccagc gcgcgggcgc
    19981 ggccgcggcc cggttcgcac ggcagggtcc tgcgcctgag gggcgcgtgc tggaggtgga
    20041 aaacggtgcg tccgtcacct atacggtgcc cggaaagatc cgcctggcca atgtggataa
    20101 agccgtggag gtatttttcc gtgtgaaccg cgtgtgcggc aattccgtca tccgcgtgac
    20161 ggcgggcagc gaacagctgg cggcatacaa gcgcgagcat ctggcccccg gcgagatgga
    20221 gcgcatcagc gtgccgaggg cactgctgga aaaggcggac ggctgcatca ccgtcagcgt
    20281 ggaggaggca tgagaaatgg cgaatatcat ttgtatcgtg tgccccaagg gctgccgcct
    20341 gacggtggat gaaaacacgc tggctgtgac ggggaacggc tgcccgcggg gcgcggagta
    20401 cggcaaaaat gagctgacac acccggtgcg tgtggtgacg agcaccgtgc gtgtggaggg
    20461 cgcggatatc tgccgctgcc cggtgaaaac cgcgggcagc gtgcccaagg agaaaatgtt
    20521 tgccgtgatg gacgcactgg acggcgtatg cctgcacgcg cctgtgcatt gcggagacgt
    20581 ggtgctggcg gacgtgtgcg gcacgggcgt ggacgtcgtg gccacgcggg agctgccggc
    20641 ggcggaataa gggagaaaat aaaaaagggg aggggcttaa gcccctccct ttttgcatgc
    20701 agggtgaatt atactatcaa gtgcaacact ttaggggaac aaaaagaagt ccataca
//
LOCUS       DAWBSB010000008        20710 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_698_length_20710_cov_3.351731, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000008 DAWBSB010000000
VERSION     DAWBSB010000008.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 20710)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 20710)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..20710
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_698_length_20710_cov_3.351731"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2402..2411
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8475..8484
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10792..10801
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3965 a         6813 c         6018 g         3884 t
ORIGIN      
        1 aaaatgattg aaaaacgtat tgtagaccgt aaaaccggac agcgcataca ggcacgcgcc
       61 gatgacagca tagttgcgtc cggcggaata ccgcttcaga taggtatacg cgcccagccc
      121 cgccacgccg aacttgagca tgaacatagg caccatggaa aacggcaccc atgcctgagg
      181 cagcagcgtc gtcagccaga aaaacggcga accgtacaga tagaacgaat acgagttcac
      241 cgccgaggta ccgaggtccg tctcccagct ccactggccc gcgctgtttt tcacgaaccc
      301 gttcatgtaa gtgtagaacg ggatctgctg gctgttgaaa tcgccgcagt actggaacag
      361 gcccttgtcc acgacgagga acggcagaaa gatgaaaaac gccgccgcgg cgcacacgcc
      421 gaacgacagc agatacccgt tcatctgctt ttcctgtttt tgctttgcaa ggctactcat
      481 aacttgttac tcctgctata atgtaaatag tttcccggcg ccggccgcgc cgaatcgatc
      541 ctaccctgat aatacgagaa aagcgaggaa aaaattgcaa tggagctgta tccctttaat
      601 gcgctgctcg cgcgcatgaa atacatcgcc cggtggggcc tgatgcgcgc cgcgcgcacg
      661 gaatccctca gcgagcacac tgcggacgcc gccattctgg cgcacacgct gtgccttgtc
      721 tctcaaacta tctttggcac ggatgtgcgc cctgaaaccg tagccgtcgc cgctttgtac
      781 cacgacgcca gcgaaattct gaccggggac atgcccacgc ccgtcaaata caaaagtgag
      841 ccgctgcgcg cagcctacaa ggctgtggag gccgacgcgg cccacacgct gggcgctctg
      901 ctgcccgacg cggtgcgcgc cggcatggac ggctatctct caggcggtat ccttacgcag
      961 aaagagcgcc agcttctgaa agctgcggac aggctctccg cactcatcaa atgcgtcgag
     1021 gaggaaaacg cgggcaaccg ggaatttgca ggtgcgcgcg ctcagcagac ggcggcgctg
     1081 gccgccatgg aatgcccgga ggcggattac ttcatgcagc atatgctgcc ctgctatgac
     1141 tggacgctgg acgagctttc cgccggggga ccgcagcccg gctgagcgcc gtcttcagcg
     1201 cgtttcccgc tgaagctccc acgcgtgctc gatcacacgg tccaggtccg taaaatgcag
     1261 catgccgcag cattcccgcc gcagcgttgc cgcgccgcgc agcccatgca tataccaggc
     1321 ggcgtgcgtg cgcgcctgct gcatggcgat atattcgccc ttgtcctcgc acatatcgta
     1381 gatatgccgg cgcagcacac ggaaccgctc gtgcaggctg ggcggtgggg gaacctcccg
     1441 gcactccatt gccgcggcga tctgcccgaa cagccatggg tttcccatgg ctccgcgccc
     1501 cacggccacg ccgtcgcacc ccgtggccgc cagcagcgcc agcgcgcttt ccgcgtccgt
     1561 cacgtcgccg ttggccagca cagggatatc cacagccgcc ttgatcttcg cgatctcctc
     1621 cgggtgaatg ccgggattgt acatctcggc acgggtgcgt ccgtgcaccg tcagtagcgc
     1681 ggcgcccgcg tccgcgcagc gcctggccac ttccaggccc gtcatcgtgt cggcgtccca
     1741 gccgatgcgc agcttcaccg taacgggcac atcggccggc gccgcgtccc gcacggctcg
     1801 ggcaatggcc ccggccagcg gcgggtcctt cagcagcgcg ctgcccgctc ccggccccgc
     1861 gatctttggc gcgggacagc ccatgttgat gtcgataaaa tcaaagggca ttttgccgcg
     1921 catatcgcaa ataaggcgcg ccgcctcggc catcacgtcc ggctgcgcgc cgaacagctg
     1981 cacgccgaag ggcgcgtccc cgcccccgcc ggccatcagc cgcgggcttt ttttgtcgcc
     2041 cattgtcaaa gcctttgcgc tcaccatctc gctgacggtg cataccgctc cgtgggcggc
     2101 gcacaggcgg cgcatcgtcg tgtccgtcgc gcccgccatc ggcgccaaag acgcgccctt
     2161 tgccagagta aatcgtccca actgcataga tcaaccaact tttttgtatt tggaaaagcg
     2221 ctctgcggga agacgccggc cgtccggcac ttccgagcga tatctttgtt attgtaccat
     2281 aaaatttcat attatggtat actatacagg aacgtttcac atttgaaaat cacggaggat
     2341 actcttttga aactgcttgt attagacggc aacagcatcg tcaaccgcgc cttttacggc
     2401 annnnnnnnn ncaagctgct caccacaaag gacggccgct tcaccaacgc catcgtgggg
     2461 ttcatgaata tcctgcttaa gctggaagcc gaggaaaaac cggacgaggt agcggtcgcc
     2521 tttgacctga aagcgcccac cttccgccac aaaatgtaca gcggctacaa ggctcagcgc
     2581 aaaggaatgc ccgaagagct ggccgcccag cttcctgtgc tgaaagagct gctggcggac
     2641 ctcggctaca aaatggtgac gtgcgagggc tacgaggccg acgatattct gggcacgctg
     2701 gccgccgcct gcgctgcccg cggcgacgaa tgcgccatcg caaccgggga cagggacagc
     2761 ctgcagcttg tggggcccca cacccgcgtg cttctggccg gcacccagat gggccgcagc
     2821 gttaccaccg tgatggacga ggccgccgta tttgaaaaat acggcgtcag gccccgccag
     2881 ctcatcgagg tgaaaagcct gatgggcgac gcgtcggaca acattcccgg cgtcgcggga
     2941 gtgggtgaaa aaacagccct tgccctcatt caggatttcg gctcgctggc aggcgtgtat
     3001 gaaaacatcg acgacgcgcg catcaaaaaa ggcgtgcggg aaaagctgct gcgggacaaa
     3061 gaacaggccg ggatgagccg cacgctggcc gagatcgtgt gcagcgcgcc cgtaaacgcc
     3121 gcacccggcg catacaaaaa agacgcgccg gacgcccctg ccgccgcacg cctgctgacg
     3181 gaccttgaaa tgtacgccac gctggacaag ctgcgcctgc ccgccgaagc cgggccgcag
     3241 cagcccggct ttgacgatgc gcccgctgtc cctctggtgg aggcggcccc cctgcccgcg
     3301 ctcacgggcc ctgtctatct ttgcgccgcc ggagacgtga tgctggccgt acaggacgga
     3361 gccgtatatt ccgcggggct tgaggatgaa gcgtttttgg cgctgcttgc aaatgaagcc
     3421 gccgaaaaac gctgtttcga cgcaaagccg ctgtaccgcg cctgctttgc ccacgggctt
     3481 gccgcccaaa acatcacttt cgacgcaaag ctggccgcct acctgctgaa ccccgcggcc
     3541 agcgattaca ccgtggcccg ccttgccgct gaatacggcg tgcgcccggc gttcagcgcg
     3601 ccctggcccg aagccggcgt gctggaagag ctgtgcgcag tgctgcgtga aaaatgcgat
     3661 gcagagggca tgggaaaact gctggacgac atcgaattcc cgctgtgcga agtgctggcc
     3721 agtatggagc acatcggcat tctggtggac aggaacggta ttgaagcctt tgggcaggag
     3781 ctgcagacgg ccctcggtgc agagcttcgc gccatctatg atgaagtagg ctatgagttc
     3841 aacgtcaaca gccccaagca gctgggcaag gcgctgtttg aggatcttgg cctgcccaca
     3901 cgcaaaaaaa cgaaaagcgg ttattccacc aacgccgaaa cgctggagag cctgcgggac
     3961 tattcccccg tcatcggcca catcctgcag taccgaacct accagaagct gaattccacc
     4021 tatgtcgagg gcctgctcaa ggtcgtgggg gcggacgggc gcatccattc caccttcaac
     4081 cagacggaga cccgtaccgg ccgtatttcc tccggcgagc ccaatctgca gaatatcccc
     4141 gtgcgaaccg agctgggcag ccgcttccgc aaatatttca tcgcgcctca gggcgaaacg
     4201 ctgctggacg cggattacag ccagatcgaa ctgcgccttc ttgcgcacat ctccggcgac
     4261 gaagccatgt gcgaggcgtt ccgttccggc gcagacatcc accgcagcac tgcggccaga
     4321 atctacggtc tgccgccgga acaggtcacg cccgcgctgc gttcgtccgc caaggcggtc
     4381 aacttcggca tcgtgtacgg catcggcgcg ttcagcctgt ccaaggatat cggcgtcagc
     4441 gtcaaggaag ccgacgcgtt catcaaaaac tatttcgcca atttcccggg cgtcaaaaaa
     4501 tatctggacg agaccgtgga gcagggccgt gaaaacggct atgtaacaac gctgtacggc
     4561 cgccgccgca cgctgccgga gcttgccagc agcaatttca acgtgcgcag cctgggcgag
     4621 cgcatggcca tgaacacccc catccagggc accgccgcgg atatcatcaa gctggccatg
     4681 gtgcgcgtat accgccgcct gaaggccgag ggcctgcgcg cccgcctgat tctgcaggtg
     4741 cacgacgagc tgatcgtcga atgtccggct gaagaaaaag agcgcgcggc caaagtgctg
     4801 ggcgaggaga tgcgcggcgc ggccgagctg cgggtgccgc tctccgcgga tgtaaacagc
     4861 ggtacgacat ggtatgcggc caaaggttga cggccccccg cgtccgcacg cccggccccg
     4921 gtggccccgg caatgaaaga caatgaaaaa aggacgaacg atatgcagca ggaaacaacg
     4981 gcccccaggg aggccggccc catcctctgc cgctttatgc gggaaaccga cgtgcccgcg
     5041 tgcgcggcca ttgaagcctg cgcggacgac ggctggagcg aagccgcact gcgcgaagag
     5101 ctggcgcagg aaaacgcgcg cctgtttgtt gccgaagccg aaggaaaagt cgccgggctt
     5161 gcggtgttcc agctcgtgtg cggcgaggca agcctctacg ccgtaagcac tgcgcccgcc
     5221 tttcgccgcc agggcgtggc acgggcattg ctgtcctggg cgttcgcggc gctgtgcctg
     5281 cagggcgcgc aggacgtgtt cctggaagtg cgcgcccaga acagcgccgc gcaggcgctg
     5341 taccgctcgc tcggctttgt gcagacgggc ctgcgccgga acttttatgc aacgcccgcg
     5401 gatgacgccg tgctgatgca gaaaacgctg taacggcgcg gcggccgctt ttcccgggag
     5461 gtgcttttac ggaacggcaa atttatctga tcacgggcgt gatggcggcg gggaagtcca
     5521 cgaccgccga ggcccttgcg ctgaagctgg acaaatgcgt ccatctacgc ggcgacgtgt
     5581 tccgccggat gatttcctcc gggcgggaag aaatgtccgc agcgccctcc gaagaggccg
     5641 tgcgccagct tcatctgcgg taccgcctga cggcggaggc cgcgaaaacc tatttcgacg
     5701 gcggcttttc cgttgtggtt caagacaact attacggcga agcgctgccc cgtatgctgc
     5761 ggctgctgca aggttatccc gtagaggccg tcgtcctgtg ccccagcctt gccgcggtga
     5821 aagagcgcga ggaaaaacgg gccaagacgg gctatacggg cttttcgccg gaagctcttt
     5881 acgacgcttt tctggcggaa acaccccgcg tcggcttctg gctggacaac acgagcctgt
     5941 cgcccgcaca ggccgcagac gcgatacttc gtcgctgcgc cggtgtaaac gggtgacatg
     6001 cctctcatcc ggaaaggaag gtatttttct atggcagact ggaattccga gctttacctc
     6061 aaattcaaag cggagcgcac acagcccgcg cacgaccttg ccgcgcgcat ccctttgcgg
     6121 gatgcgcgca gcattctgga cattggctgc gggcccggca acagcacggc cgcagtggcc
     6181 gcgcgttacc ccggcgcgcg tgtactgggc gtggacagct ccgaaaacat ggtggctgcg
     6241 gcctgtgccg cacatccgga atttgaattt cgtgtgtgcg aggcaccgca gggcctggac
     6301 gcactgggcg gcggctttga tattgtattt tccaacgcgt gcctgcagtg gatcccggag
     6361 caccggacgc tgctgcccgc gctgatgcgt cagctccgtc ccggcggcgt gctggctgtg
     6421 cagatccccg tgcagcaaag ccagcctatg cacaggatta ttgccgaaac cgcccgccgg
     6481 gaaaaatggc ggacggcctt tcccgcgccg ccccgcgcgt tccacaatct tcagcagaat
     6541 gaatacttcg atgtgcttgg ggagctgacg caggaattca cgatgtggga gatcacctat
     6601 tatcaccgca tgcccagcca tgcggccatg ctggagtggt accgggccac aggtatgcgg
     6661 ccctatctgg acaagctgaa caaggcgcag caggaagagt tcgagcgcga cgtatccgct
     6721 cagcttgtgc aggcctaccc cgcgcagcgc agcggcgaga tcatcttccg cttcccgcgc
     6781 ctgttcttca cagccgtgcg gcccggcgcg taaaaagggg ccggaatatg aatgaaacga
     6841 tctaccgctt tgaagcggtg ctgcaaaaag tgccggacct gaacggcgcg tatgtggaat
     6901 tcccctacga tgtgcgcgcc gaatttggca agggccgcgt gaaggtccac gctactttcg
     6961 acggccagcc ctatgacggc agcctcgtgc gcatgggcac ccccgggcac atcctcggcg
     7021 tgcgcagaga catccgcgcg aagatcggaa agcagcccgg cgacgtggtt tccgtcactg
     7081 tacaggaacg gcaatgaccc gtttttcatt gttttcaaag gaggacgccg caatgaaaaa
     7141 gaacctcttt cgctcccgcc ttttcctctg tgcacctgcg gcattttgcc tttgcgcggc
     7201 ccttttgtgc gcctgctccg cccagggcaa cgctgttcct gcctccgtac atgaacaggc
     7261 gcttgcgcag ttgaaagcgc aggatgctga attgcaggcg ctcaccgaac aggtggctga
     7321 attgaaagcc gcgctcgcag acgcgcagcg tgcggccgtg ttggaggata cgcgaaccga
     7381 gcgggaaaag cggctcgcgg ccgatctata cgcccaccct gagctgattc ccatcgaggg
     7441 aacgctgggc ggaaccatgc gcttttcccc ggatgagagc gccgtccgtg tgctttccac
     7501 cgcaagctat atgccgctgg tatacgccta tgccgaggac ggccacaccg cggtaaacct
     7561 tttgttccgc tttgaaaacg cggcggacgg cgcgctgaaa tggcgctgcg tcgcttacga
     7621 ccatggcggc gggcttacgc tgttggagcc gcaggccgaa taaaccgaca aggaagtttt
     7681 atatgtatat tttgggaatc gaatccagct gtgacgatac ctgcgccgcc gtcgtgaagg
     7741 acgggcggga gcttctttca aattgcgtgg cctccagcgc cgcagagcaa aatctttacg
     7801 gcggcgtggt gccggagatt gcaagccgcc gccacatcga gcatatctct caggtggcgc
     7861 gggccgcgct ggacgaagcg ggcacggcgc tggatgaaat cggcggcgtg gcggtcacct
     7921 ttgcgcccgg gctcatcggc gcggtgctgg tgggcctgaa ttttgcaaaa ggcctcgcgc
     7981 tttctgcgca aaaaccactc atccccgtgc accatctgcg cggacatatc gcagcgctgt
     8041 acctgacgca ccccgagctg gagccgccgt ttttatgcct cgttgccagc ggcgggcaca
     8101 gccatattgt gcgggtgcag gactacaccc atttcgaggt ccttggccgg acggtggacg
     8161 acgcagccgg cgaagccttc gacaaggtgg cgcgcacgct ggggctgggc tatcccggcg
     8221 gcccggcggt gagccgggcc gcccaaacgg gcgattccaa agcttatccc ctgcccacgc
     8281 cccatgtaga gggcccgtac aacgtcagct tctccggtct gaaaacagct gtgctcaatc
     8341 ttgtgaacaa agcgcagatg aaaggagaac gtgtgaacgt ggccgatatg gccgccagct
     8401 tccagttccg catcacggac attctggccg aaaagctgct gctggccgcg caggataccg
     8461 gcgcaaagca gatcnnnnnn nnnncttgcc gcgggcgcga agaagctggg cgcgcggctg
     8521 tatctgccgg agctccggct ctgcggcgac aacgccgcca tggtggccgc acagggcttt
     8581 tacgaatacc gggcgggcaa taccgccggc ctaaatctta acggccttgc cacgctgggc
     8641 atcgattacc tctgaactgc cgcccccatc gcatcaaatg aaagccgccg ctttcacgca
     8701 agccgttcgg cccacggaaa acggtggttt ttctttccga cctgggttca gacgttacag
     8761 cccgtcccgc agccttgatc ctgcaagcgc cgggcatgaa gtgcccgtac gcgcataaag
     8821 cggcagctcc gcctcggtat tttattgctt ctcaataaaa acccccgtgc aatccactcc
     8881 gcttttcgat acaatatacc gtgtcggcca aacactgctc cgccgatacg ggaaaggagt
     8941 tgtttttttg cataccaaag cgcctcacga gcagttttac cgctacgtca tccccgccat
     9001 ggcatcccag cttctgacgg gattttttat catcgtcgac ggttttttca tcggccaaag
     9061 catcggggac gtaggccttg cctccatcaa catcctgtgg cccatcgcgg ctgtcatcct
     9121 tgccacgggg ctgggcatcg gctccggcgg cagcgtggtg atgacccacg ccatgggcgc
     9181 gggcgacacg gaaaaggccc tggccgcgcg gggcaacacg cttttgtgcc tgggtgcggc
     9241 caccgtgctg ctgacggcgg cgctgggcct ttcatacccg tggctgctcc gcctgcttgg
     9301 cgcaacaggc gagctgtacc agcccgcggc ggattacacc gttgtcgtcg tgctggcggg
     9361 cggcctgcag atattcaaca gcggattgaa ccctctgctg cgcggaagcg ggcgtaccgt
     9421 cgccgcaatg gccatcatga ttttcagcct gctgtgcaac atcctgctgg actggctgtt
     9481 catcgccgta ttcgcctggg gcatgcgcgg cgcggcgctg gccaccgtaa cggcgcaggg
     9541 cctttcggcg ctggccgcac tgctgtgcct gcttatgaac aaaaacgctc ccgtccacgc
     9601 gcgccagttt tgccccgatc cgcgcctgtg caaaaaactg cttacggtgg gcgtttctcc
     9661 gtttgggctt tccatgtccg ccagtgtgct catcatgctc aacaactggc agtgcatccg
     9721 ttacggcggc gcgggcgctg ttgctaccta cgccatcctc agttatgtac tgggggcgct
     9781 gcagccgctt ttgtccggta tcggcgaggg catccagccg ctggtgagct tctgccacgg
     9841 tgcgggcgac cttgcggccc agcatcattt acagcgccgc gcgctgcgtt tactgctggc
     9901 ggtggccgcg gtgctgtgcg caggcacatt ccttgctcgg gaacagctgc cgctgctgtt
     9961 tggcgcttcg tccgagaccg cccgggaaag cgttcccgcc ctgttgtttg ccgtcgcctc
    10021 gctgcctctt tggggcgtgg tgcgcctgta cgcctcctgc ttttacgcca ccgggcagac
    10081 gcgccgcagc ctgttcttta tttttggcga tccgcttggc gcaagccccc tgtttctgtt
    10141 ccttctgccg ccgatgctgg gcgtgaacgg catctgggcc gcggctcccg cagcacaggc
    10201 gctgctgttc ctctgcctgc tgggcatgtt ccgccaggat ggccgccgca gcgtgcagcg
    10261 cacctgaaaa cgccgttttc ccacgggcct ccggcctgtg caaccaccgg ttgacacatt
    10321 gcccggcgcc ggttggtggc gcgcaaccgc ccggcggggt atgctggaag caccccggag
    10381 cgcaccaggc cccggcaaca aaacagtgag gtgcagtgat atgatttttg cggataaggt
    10441 cgtccagctt cgaaaaaaaa gcggctggtc gcaggaggag cttgcggaaa aactgaatgt
    10501 aacacggcag tccgtctcca aatgggaggg cgcacaatcc attcccgacc ttgaaaagat
    10561 tttgcagctg gcgcagatct tcggcgtcag cacagactat ctgctgaaag acgagctggc
    10621 ggaagccgaa tacacgaaat ccgatgattc ctcctcggtg cgccgcgtct ccatggagga
    10681 ggccaacgct tttcttcagg tgaagcaggc tacggccgga cgcatcgcct ttgccacttt
    10741 tttatgcatc ctctccccca tctgcctgtt cctcttggct gccgccagcg annnnnnnnn
    10801 nacgggcatg ctgcccatcc gtgaaaatct ggccggcggc gcggggatga tcgtgatgct
    10861 cctgctcgtc gcagttgctg tggcgatgtt catctcctgc ggcggcatga cgagccctta
    10921 tgcctatctg gaggccgaag tgtttgaaac ggaatacggt gtatccggca tggtgcggga
    10981 acgccaacgt cagtaccgca gcacctacac gcggtacaat gttttgggcg cctgcctgtg
    11041 tatcctttct gccattcctt tgttcggcgg cgcgttcctc tcggaaaacg ggctttttct
    11101 tgtgggcatg ctctccgtca tgctgctcct aatcggcctc ggggtgattt ttttcatcgt
    11161 gggcggcgtc aactgggcca gcatggaaaa gctgcttcag gagggcgatt atacgcggac
    11221 aaaaaagaaa accagctcta tcaccggcgt ggtgagcacc gtgtactggt gcgtggcagc
    11281 ggccgtgtac ctggcatgca gcctccccgg ccattcctgg cgggaaagct gggtcatatg
    11341 gcctgtggcg ggcgtgctgt ttgcggcggt gatggccgta tgcagcgcat tggccgggcg
    11401 aaaaaaataa aaaggacgca tcagaaaagg gaaagcggca gattccgctt tccctttctt
    11461 tcattgcttc accgtttcag gctgcgctcc gctttcagca gatggctctc gtaaccttct
    11521 atctttttgt ccagcctgtc caatacggcc tgcatctcgc cgatgcgcgc agccagttgg
    11581 cggcgctgtt ccatcagcag ctctttgcgc gcgggaatgg tctcgtctcc ctgtccgaac
    11641 aggcccacat attcaatcag cgtctccacg ggaagtcctg cgccgcgcat gcatttgatg
    11701 aaattcaccc agttgcagtc ctcttcggta taatcgcgga taccgctctt tgtgcgcccc
    11761 accgggggca gcaggccgat gcgctcataa taccgcagtg tatccgctga gacgccgtat
    11821 ttctggctta cctgtgcaat cgtcatatca caaatactcc ttttatcccg gcgcgctccg
    11881 ccgccgttct gttcttcaaa agccggcctt tgccgcacac tatgcgccga aggtctcctc
    11941 tgcccggcgc atgttttcca ccgcgtccac gccgaaaggg cagcgcttgc tgcatgcgcc
    12001 gcaggcgata cattcgcccg ccttatgggc cagcgccgca taatgctcgc gcaccgtctc
    12061 cggcaccatg ccctgggcac gggccaggtt cagaaatttg gtgacgcttg caacgtcgat
    12121 cccctttgga cacggggcgc agtggccgca gtacatgcag tgtcccctcc agcttatttt
    12181 cgggaaagtc gccagcgcag cagcgtaatc ccgcgcgctg tccggtgccg tctcataagc
    12241 caggctggcc gcaagctcgg cggacgtgtg cacgccggac atcacgctgg ccacggcggg
    12301 ccgcgtgagc gcatagtgga tgcactgctc caccgtcagc gcaacgcccg ccggggaatc
    12361 cgccttcagc aggtctcccc cgccgaaggc cttcatcacc gtgatgccca cacccaggcg
    12421 ctggcaggtc tcgtacaggc gctcccgcgc agggtccata ttcaccagca tgcccgcata
    12481 gctttcctcc gcccacagag cctcgcaatt ctctcccgcg ggctgcagat catagcaggg
    12541 gttgacgctg aacatcagta cctccacaag gccgctttcc acagccgcca gcgccacctc
    12601 cgggttgtgg ctgctcaggc cgatgcaccg gatgcgcccc gcgcgctttt gctccagcgc
    12661 atagcgcatc acctcgccgt ttgccacagt gttccatgtg tccatggtat ccacataatg
    12721 gatcattccc acgtcgatat agtctgtttc caggcggcgc agcatgtcct caaaggaagc
    12781 ccgtacctcg ccgatgtccc gcgtggcttt gtactggccg ttctgccaga cggtacacag
    12841 atgcgcctgc agcacgaact gttcccgcct gccccgcagc gcgcgtccca cgcggctgcg
    12901 caggtccggg tccggcgtgt acagatccat acagttcacg cccgcttcca ccgcctgcgt
    12961 catcaatgcc tgtgtctctt cttccgtctt gcccacaaag ccctcgcacc caaggccgat
    13021 ctcgctcacg cgcagcccgg tgcggcccaa ttccctgtat tccattgtat tctccctttt
    13081 ctttttgcgg gcgcttacgc ctgcgtttca aacagcgccg cggtttcctt taaatggcgg
    13141 atcaccggca gatgctgcgg gcacgcccgc tcgcactggc cgcagccgat gcactccgat
    13201 gcttttcccc cgttctgcgt caggtttccg taatagacgt cgctgttcca gtctccaaac
    13261 tgttttttgg cgttgtaaca gccgaacagg ccggggatgg gaatatgctt cgggcaaccc
    13321 ggcacacagt aacggcaggc tgtacaggga atgctgtcct gctgtttcaa aatctcccgc
    13381 acacgttcca cagccttgta ttccgcgctg gtaaagggct gaaaatcttt catgtagctt
    13441 acgttgtcgg caagctgttc atagttgctc atgccgctta gcaccatgaa caccccctca
    13501 aaggaagccg cgtagcgcac ggcatagctg gcgtagcttc ccgttcccac gccgtccagc
    13561 accgccttcg cctcctccgg cagctggata agtcccccgc ccttgacagg ttccatcacg
    13621 atgacgggct tttggtgtct gcggcacacc tcgtacacgg cacggctctc gatgctgggg
    13681 ttttcgtaat ccgcataatt gaactggatc tgcaccactt ctatctccgg atgctcgtcc
    13741 agaacgcggt ccagcagagc aggcttgtcg tgaaacgaaa tgccgatatg cttcaccctg
    13801 ccctgctcct tgagctgttt cacgatttca aacgcattgc agcgtgtaaa tttttcgtag
    13861 taagcgccgt tcagcgcgtg cagcagataa taatcgaaat aggagacacc ggttttttcc
    13921 agctgctgct caaataaagg caggatatcg tcctcctttt caaaaaagaa cgacgtcaac
    13981 ttgtctgtca aaatgtacga ttcgcgcgga tagcgcgccg tcaggcattc ccgcagcgcc
    14041 gtttcgcttt tgccgttcaa atatccgtga gccgtgtcga aatagcaaaa gccctcggcc
    14101 agaaacgtat ccaccatgcg gcagaattcc cgcttgtcca cttcgccgtc cgcaccgccc
    14161 atcatgggca gacgcataca gccgaatccc agttttttca tattgcattt tcctctctta
    14221 cccatgccgc acaccggcgg gcggcattac aagctttgta gtaagcataa catctggagt
    14281 tcactccaag tcaagctttt ttgcaaagaa aagcaaaatg caccgcctgc acaaagaagc
    14341 ctcccgccct gccatacgca gggcgggagg cttcttttgc tgtacacagg gtctgcgtca
    14401 tccccacacg ggaaatgaaa gctcggccac cgctccgccg ccggccgcat tgcgcagcgc
    14461 cagcgcgccg ccgtgacgcc tggcgacatc ggcggcaaac gtcaggccca gacccatatg
    14521 gccgtcctgc gggcggcttt tttcgcccgt gtacagcggc tgcatgcctt tggcaagcgc
    14581 ctcgggtgta aagcccggcc ctgtatcccg tacggtgaac accgcacggc ccgcgccgtc
    14641 cggggcgcgc ctcacgcggc tttccagcgt taccgccccg ccctgcggcg tgtagcgcgc
    14701 ggcattctcc acaagattca ccaacgcacg cagcagctcc gcccggcgca tgggcagcat
    14761 ggacgttcca tccgtcagca ggcgaaatac acagccccgc gcccggcaga gagcctggcc
    14821 ctgggcttgt atctcggcca ggaactccgg cagggagacc cgctccgtct cctgcggagc
    14881 ggcatgctcc tgcgcgccgt ccgacgcagc gggctccggg cttttcaccg tatccgcact
    14941 gtccgcaccc gcagccagcg ccctcagcga ggcaatgtat tcccccgcgt agcgcgtatt
    15001 gcgcaaaatg gcatctacat tcgcgcgctg ggcgtcgctc agcgcgtcct cggccagcag
    15061 ttcggcgttg ccgccgatga tcgtgagcgg cgttttcagg tcatgcgcca gcgcggccat
    15121 ctgctctgcc cgctgctgct ccatacgcca ctgggcctgc aaagaaccgg aaaggttctc
    15181 cttcagcgtg cgcatggcgt ccattgcacc gtccaactcc cgcacgcccg ccgaggaaaa
    15241 atctccgccc tccagttgcc cctccgcaat gcgtgcgctg gcggcggcca gcttatccgc
    15301 tgcacggcgc agcctgcgcg tgtaatgccg tgtgataagc gttaccgttg tcaccagcac
    15361 cacggcgagc agcaccaggc tggcaagctc aaaatccgga agaacgccgc gcagggcctc
    15421 gttgccgtaa agcacgctgt aatcgaactg aaacaggcac agcgacccat cgctgaaatc
    15481 cacacgctgg taaaaccacg cccctccctc caacgtccgc cgggcctcct tcgcgtccgc
    15541 cagccgacgg ccggaaaggt tcgtctccag cactgtgccg tccggggcga aataaacata
    15601 attgcacagt tcgtccgcca gcgatgcgtc aaagctgccc tgcaggcgca ttgcggccgc
    15661 cgcgtccgag gcatggtcac tctgcgtgcg ggcgggcagc acaaaacccg cgttcatcag
    15721 cagcatgagc gttatccacc atgccgccgc cagtgctgct gccgaaacgc ctgtcaatat
    15781 caaatagcgc cacagcagcg cacccaggct cacatttctt cgtttttcca tttgtacccg
    15841 atcccccaca ccgtttcgat cggcgcgccg cccgccgctt tcaatttagc gcggatgttt
    15901 ttcacatgct cggtgacggc ggaatcgtcc gcacagccgt catagccaaa taccgcctcg
    15961 tagatctgtt ccttgctgaa cacctgtccc gcgtgagtgg ccagatgctc gcagatggcg
    16021 tattcgcttt tcgtcagcgg cagcgccgca tcaccgtggt acagcgtttt ggaagccagg
    16081 tcgaaatcaa acgcaccgcg ccgcacgcgg cgggtgggta cgcgcgcctc ccgccgcaga
    16141 tgcgccgcca cacgggcgcg cagctccgcc acgcgaaatg gcttcgtcag gtaatcgtcg
    16201 ccgccaaggc ccagtccgcg cagaacgtcc gtttcctccg ctcttgcggt aaggaacagg
    16261 atagggcacc ctgccagggc gcgaatgcgc gcgcaggtgg caaagccgtc ctcgccgggc
    16321 atcatcacat ccagcaggat gcagtgcgcc caggcgcagt gctccggcgt cacctgcgct
    16381 ccgcatgcca acgccgttac ggcatgcccg tcccgctcca atgcgccgcg cacaagcgcg
    16441 cgcatgtccg catcgtcgtc taccaccaga atgtgtgcca cagtctcgcc accatttcaa
    16501 tcagtcattg catccataaa tacaaaagcc acgaaagccc gtacaaaaca tagacgtgcg
    16561 cgggatagaa cacataaaag aaatacttgc cgccgcgccc ggaggcgccg cgctgcccat
    16621 tgtacagcag catgaacaca gctgaaaacg cgcccatcca ctcaaacgcc tgattaaaga
    16681 taaaatccca cgtcgcaccg gggtaaagca ccgccgtgct tccgtacacc agaaggtaaa
    16741 atgcggcgaa agccagcgcc tccagcttcg tgcttttcag ccggcacaaa tacatcagaa
    16801 ggccgcacag gataaacagc gtgccgccgt ccatgatcat ggtgtgcatg ggcagcaccg
    16861 taaagcccag cagcgtcagc cagccttccg cctgcgggaa catcggatat atcaccatca
    16921 gcatgaaaaa cggccaggcg aaaggcagca ccaatgcggc cagcccgcgc agccaccgct
    16981 tttgccgcag ccagtccaca ccctgcagca tgggcagcag aacggcaaag gtcgccagca
    17041 tctggttcat cggataaaag ccgtcgggac gcctgcagaa atcgaacaga atgcagcaga
    17101 actgcactgc tcccatgccc acggaaacaa ggtaaatgcg cagaaagtag cgccgtctgt
    17161 catgggtgtg gatgaagccc tcgatcaggc agaacagaaa cagcccggca gaaagccgcc
    17221 ccagctgcga aaacaaaagc ggtaccgcgc ctgtgaactc gaaaaaataa tggatatggt
    17281 ccagcaccat gagcaccagc gcgatccatt tcagctgcgc agtggtaagg ccacggcgca
    17341 gccccgtttt ttgcattgtg ttttccaaac agatacactc ctcttttttg ttgctggaac
    17401 gagtatatcc gccgtttcta aggaatttat aagggcgcgc aaaaatcatt cgccgcgagc
    17461 cgcctccagc gggaccggta tttccctccc caggctgaag gaatagatgg tgcaccgctc
    17521 cacgcgcacg cccaaacgtt tttccacagc tgcccggtac agcagaagct gctttgcata
    17581 cgcctgcaaa agctgctccg gcgttttgcc acggtccgtt ttataatccg cgatctccgc
    17641 cgtatcccca ttcaccagca ccgcgtccgc aatgccctgc accagcacag gctggtgcgc
    17701 gaacggttcg ggcagcgtat cgtccacgaa cttcgcgggc accgccgtaa taaaatcgta
    17761 ctcgcgcagc agcttcttag cctcgcgcat gcgtgccgcc aacgggcttt gcaggaatgc
    17821 ggccatgcgc ggcagctcca gctgccctgc cagcgctgcg tccagccagc cgccctccac
    17881 aaggcgcgcc acctcgccgg gcaggtcgtt tgccgcagcg gcaaggtccg cgaactgcag
    17941 cgccgcatgc agcgccgtgc cgcgttccgc cgctgtcagc ccctctttgt aaaggaacgc
    18001 cgggcgcgcc agaaccgggg ccgcgccctc atggacaagg cctgaaacac tcagctttgc
    18061 gggcagcgtg cacaacggcg cattttcata ccgtgcgccg aaattctgca aaagctgttc
    18121 cagcagcgct gcgtccggcc gggctgtgcg cacaaaggca gacggctcat ccgccgcctg
    18181 cccggcgggc tgcgcgggca ctacgcgcgc gcgcatatgc ccccgcgccg catagacagg
    18241 cagcaatgcg ccgcccgcgt atttgcgcag ctcttcgcag tccgggtgca gcagcgcggc
    18301 cgtacacagc cattcgctga acgaaagcgc ctgtgacagc gtgtacggct ccgctccgcc
    18361 cgtgcccgcc agcgtggttg ccagcgctga aagtgttttt tccgggtttt gcagcggcgc
    18421 cgtaacaatg agcttgtccc gggcgcgggt gagcgctaca tacaggatgc gcatttcttc
    18481 gctgacggcc tcgctgcgct gcgccatacg caccgcgcgg tggggcacag tggaatacag
    18541 cccgccgtcg ccgtccgcac gcaggttcat gcccagcccc agcgacgggt ggaacagcac
    18601 cgggttggag gtatcgcgca gattgaaccg ccgggctgtg tccgccagga tgaccaccgg
    18661 aaattccagc cccttggagc ggtggatggt cataatggaa accgccccct ccggcagcac
    18721 cggcgtgccg ccgctttgca cagcgccgct ttccaccgcg ctgtccacca cccgcaggaa
    18781 cgcggccagc cctcccgcgc cgccccggtc cacaccggcg gcccaggccg taaacgcccg
    18841 cagcgtctcc cgccgggcgg ggccgttttc catcgcgccc acagccgcga agtaatgcgt
    18901 gcgggcgaat atctcgccgc acagctcctc cacggacgcg gtggcggcca gccgccgcag
    18961 cgcccgcagc gtttccagaa aaacctgtgc ctttggctct tcgcttttca gcagcgccgc
    19021 gtacaggctg ccgcgcgggg ccagcaggcg cagacgcgtc aaatcgtccg gcgtgaatga
    19081 aaacatcacc gataccagcg ccgcggccag atgtacgtcc tgtccggggt tgtcgatgac
    19141 ccgcagcaga gaaagcacgg gagagacctc cgtggacgta agagggctct cgcccgtgtc
    19201 tgcaaaggac ggaatgcccc tccgcgcgaa ctccgccgca taaaggccga accgcgcacg
    19261 gctgcgcagc agcacgcaga tatcaccgta gccgcaccgc cgcgtgccgc cgcccttttc
    19321 gcgcacggca aagccttcct tcaccaggcg ctccaccgca tccgccaccg cgcccgcgtc
    19381 tcccatggcg gtgtcgccgc cgctcatgtc cacaatatcc agctccatgg ggccgccgtc
    19441 gtatccgtcg tccgcgccgc gcaccagcat ttcgtccccg ttgtagtcca cgtcgcccac
    19501 tgtgcggctc atcacacagg caaacacatc attgatctgg tcgatgatat tgcccgcgct
    19561 gcggaagttg tggcccaggg tgacagtggc cgggtgaggg ccgccgggcg tataaggcgc
    19621 aaaaccgccg cgcttgccga tgaatatctc cgggctggcc agacggaagc ggtaaatgct
    19681 ctgcttcacg tcgcccacaa aaaacaggtt ggagccgtcg tcgttggcga ggcactgata
    19741 caaaagcgcc tgcagggcat tggtatcctg atattcgtcc acaagcaccg cgtcgaaccc
    19801 gcgggacacc gtgcgggcca cagccgtttt ttcgccgttt tcaccgcaca gcagctccag
    19861 tgccaaatgc tcaaaatcgc tgtactccaa tgccttttcc gccagcttat cttcgtacag
    19921 ccgcgccccg aaggcctttg ctccacgggc cagagccgca gccataggcg caatgcgggc
    19981 acggtcttct tcaaattcgg cctccgtgca caggaacaca tcttttttca aacgctccgc
    20041 agcgtccttg gcggcggcgc gcagcgcctt caccgcattc attcccgggc tttgatatcc
    20101 gcgcacagcc ttgaacgcgg gcggcttgta ttcggccgta taaacagccg cactgtccca
    20161 ccggcccgcg ccgatgtatt ccagcagcgc cgcgaaaaac gccgcgtcgc tgtccagcgc
    20221 cggtgcataa ttggcaagct ccggctcctg cgcgacgatg cccttcgccg catccagcag
    20281 gcgcagtgcg ctgcgcgcgg cccgcccggc gttttccagc aatgtacggc cccatgccgt
    20341 ttggcccagc ggctgtccgc tttcgtaggc tgcgcagatg ctttgcagcg ccgctgcggg
    20401 atggggcagc gtgcgggaaa aatcatacag ccccaacacc gcggcagcgg cgctggcgtc
    20461 gctgcgggcg cggccgtaca gtgaggcaaa cgcgcagaaa tccgcgtcct cataaagctc
    20521 ctccagcacg gcggaaagcg cgttctggcg cagcgtatag gcctttgcgt catccgccag
    20581 ctcaaaatcc ggcggcaggc ccagctccgc gaaatagcgc ttgagcagct gcatacagaa
    20641 tgcgtcgatg gtacaaatgt tggcgcgccc cagcagcagc cgctgacggc gcaggtacgc
    20701 actctcgggg
//
LOCUS       DAWBSB010000009        20691 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_699_length_20691_cov_3.424598, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000009 DAWBSB010000000
VERSION     DAWBSB010000009.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 20691)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 20691)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..20691
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_699_length_20691_cov_3.424598"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    11046..11055
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    15472..15481
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3687 a         7009 c         5984 g         3991 t
ORIGIN      
        1 gaaaaaggtt ttggcggcct gtgccgccgc gcgcttttgg cggaagactt cttccgcccg
       61 cgcatatcaa agagcgttcg gctccttttt atctatattc gtgccgctct ttgtggcatg
      121 cttttagtat agagggaaga tgtgaacaaa gtttgataga aactttaacc tttttgtaat
      181 atttgatgaa tagggcttga agtgatacaa tatcagacag gaaaggaggc ggtggcatgg
      241 ctgtgaatta tcagctggag ctggaacgtg aactgcagaa aaacgagggc aaaacgcccc
      301 gcctgctgct gcactgctgc tgcgcgccct gttccagcta tgtgctggaa tatctttcgc
      361 cccatttttc catcacggcg cactactaca atcccaacat cgacaccccg gaggaatacg
      421 cccaccgcgc gcgggagctt tcccgctttg tgcaggagat gcctctgccc ggcgcggtgg
      481 atgttttggc ggagccttac acgcccgcag aattttacgc ggccgttaaa ggactggagg
      541 acatccccga gggcgggcgc cgctgctatg aatgctaccg gctgcgcctg cgccgcgccg
      601 cacaggccgc ggcggcgggc ggatacgatt atttcaccac cacgctgtcc atcagcccgc
      661 acaaaaacgc cgtttggctg ggtgaactgg gagaagcgtt tgccgcggag tatggcgtgc
      721 gctggctgcc ttcggatttc aaaaagaaaa acggcttcaa gcgcagcacc gagctggccg
      781 cccggtacgg cctgtaccgg caggattact gcgggtgcgt gttctcccgg cgcgaggccg
      841 aggcccgcgc ggcgcgcaaa agcgcgcaga ataaacatgg cgcttaaacg ggcccgcaga
      901 cattgagatg tatcgttttg cggtacaaaa agctccggct gcagagaaat gcagccggag
      961 cttttaattg tatttttcgg ccgtcccgct ctcacagcgg gaacaggggc agttcctcca
     1021 aaaagatgat gtccccgcgc ccggccgctt ctccctcggc caagatcgcc gcctttgcca
     1081 cgatctccgc accggccttt tccagcaggc gctcaatggc gctcagggat tctcctgtgg
     1141 agatcacgtc gtccacaatg gccacgcgcc tgccctttac ggccagcgcg tccttgccgt
     1201 ccaggcacag cgtctgcgcc ttctgagtgg tgatggatac cacttcgtct accagcggtt
     1261 ccgccatata gggtttcacg cttttgcgcg ccacgatata gtacggcagc cccagcagac
     1321 ggctgacctc gtgcaccaga gggatgccct tggcttcagc tgtgacaaga taatccacct
     1381 tgggcagctt ttgcgccagc aagggcgcgg cggctgttac cagctcacag tcgcccagga
     1441 tgacgaagct ggcgatggcc agctccggcg agatgggaat gataggcagc tggcgcgtta
     1501 cgcccgccac attcagctca taacaatcag acataaatcc aattcctttc tctgcacggc
     1561 tcgcgcgccg cctgttacag gcccagcccg gccccgaaca acagcttgag ggcgatgccc
     1621 gccagcatgc cgaagaacgg gtctgtaaac gctgtcacgc ccatggtaac gcccgctacg
     1681 atggtgtctc ccgcacctgt gcccgcgaaa gccgccgccg cgttgcccgg caccgtcacc
     1741 accgcgccca ggatgagcag aaagcccgcg atggactggc tgggcacgta tttaccgatc
     1801 ttgggcagca ggccgcaaaa cagaatgacg gccatgatgc ccatcatcag cacacccgcc
     1861 gtaacgggat ggggcgcgcc cgccgttgcg gagatgatcg cctccacagg cgcgccgccg
     1921 aacaacgcgc tcaccgcgtc ggccaggccg gagtatatcg taagatggtc cacattctgc
     1981 gaggcacccg cgatgccgcc cgtgatggag ccgaacgcga tgttcgcgcc cacggtcagg
     2041 caggccaatg ccagcgcacc gcgcagtacg ctcaggttaa aaatgggttt ttccagcttc
     2101 aggcggccca ttttttccgc cgtgcccacg ccgccgccga tgtcctgtcc ggccagcttc
     2161 gccgccgcgc tggacaccac cagcgagatg acgatggtat acaccagatt ctgtcccaga
     2221 aagaagtaga ccagcaccgc cgtaaccacc gaggtaatgc caaccaattt gttttccttc
     2281 atcatgccca gcgccagctt cgtcagcacc aggcccacgc ccgccatcat ggcgttcagc
     2341 acggtttcgc ccgcaaagtt cacaatggcc gtaagcagcc cgcacgcgcc cagcgcagcc
     2401 atcgtcacgc ctgcgaagaa taccatcgaa aggcgctcgc gcatgttttt gcccatcgtg
     2461 cccgccagca cgatcgtctc ggcctggaaa gagatcggag ccaccgaacc aagcagcagg
     2521 cagccgataa cgcccacgcc aaaccccagc gccgtcggta ccgaggcaaa gcccatggaa
     2581 agcgccagca gcccctgcgg gatgccgttg agcacgacgc cgatcgccgc aaggatatcc
     2641 tgtaaaaatt ccatatccac acacctgttt tctttatttg gcctttcccg cacgggaaca
     2701 gccattttca ttataacggc gcgtgtacgg gcgtgcaagc atttggcaaa aaagcgcagc
     2761 ttttgtcacg caaaaaactg cgcgggggcc ttgttcagcg ctttaggagc gcgcccccgg
     2821 ccgccgcatc caccagccag gtgaagatgg ccgcatggtc cggatgctcg ggcgcaagat
     2881 tctccgggtg ccactgtacg cccagcgcaa ggccgtccgc gctttccacc gcctccacaa
     2941 ggccgtccgg cgcgtgtgcc gccgccgcaa agcccggcgc aacgtcccgc accgcctggt
     3001 ggtgaaacgt gttgcactcg tgctgccccg gccccatgat acgcgccagc aggctgtccg
     3061 gcgccagcgt caggctgtgg aacagttcgc cgcgcgccgc actgtcctga taatggcaga
     3121 tagccgcccg gcactgcgcg gggatgtcct gccacagcgt gccgccgaat gccacgttca
     3181 gcacctgtgc gccgcggcag atacccagca gcggcttttt atgctgcgcc gcggcgcggc
     3241 acagcgtcag ctccacgttg tcgcgcaggc ggtcggtgct ggtgagctgg ggcaccgggc
     3301 tctggccgta ataggtgggc gcaacgtcgc cgccgcccgg cagcacaaag ccgccgcaca
     3361 gcgccagcca ctgggccatg cccgcgctca cggcgtcggg cgtggcgggc agcagcagcg
     3421 caaggccgcc cgcgcgttcc accgcgcgca cgtaatcgtg ccctgtggta taggcggggt
     3481 gcacctcccg cgtgcaggcc gcgatgatcc cgattaccgg cttcatgctt tcacgcttcc
     3541 cttcggcgca gcgcaggctc ttcgcccgca cgcacacata ctctctattg atttattcca
     3601 ttgttgttgg ccttgtgtca aagcctctgt tggcggcgtg ttggaacgcg ccggaacagc
     3661 cttgagataa caatgtgttg gcaagcgcgg aggcgcttgc cggccaaccg gtaaaaagcc
     3721 tgccgccatg ccgtttccgt cgtatggggc agccggcttt cggcctgttg gcaagcgcgg
     3781 tttttgcgtg gtcgccggca cggcttcggt atgttggctt gcccgcgcgc cgcgtgtgag
     3841 ccgtgggcta ccttccatgt cgattttccc gcccgcgcca tccgcctttc aagcgcgccg
     3901 caagcgcttt tttaccatca gccgaaggga tgcaccacac ccgtgtttcc gctgctctgg
     3961 ggcgtggagg aagcgccgcc cgcatccggc gtgcccgagc cgccgtcccc ggcgggctgg
     4021 ctcacggatg catctgttcc cggcacggaa gccggctctc cggagctggc actggtactg
     4081 atactggtgc tgccgctcac aggcagcgcg ccgcccgcgc cgttcccgcc gcccagtgaa
     4141 aagtgtgtga agaaatacca gcacgccacg gccagcgcaa ccagcagcac ggccagcacc
     4201 gcgatgggcg cggcatattt gctggagcgg cgctttttca gcggtgcgtt ccagtcaaaa
     4261 tcagagtttg ccttgaacat ccgcgcccgg tccacaggct gcttctgcgc caggcggcgc
     4321 accatgtcca gcgccgacag cgccaccacc ttacccgcgc cctcgccgtt cttgatgcgc
     4381 ttgatataca catagccccg gtgcgccacg gccaggtaaa ccacctcggc tccggtgccc
     4441 atgcccacgc tggcaacgcc gaaatccgca tgggcgaacc gggcggccgc cgccgcgcgt
     4501 gccgccgcga tctgcggcgc attgccctcc tcggcatatt tgtagacggc attcttgatc
     4561 ttcgccacaa cgcgggaatc gttgtagctg ccgtcgccga aatcaaatac gctggggccg
     4621 cgcttggtgt gggagaattc ctccgccagc agcgcgcccg tggcggggtc cgccgcagcg
     4681 atggtggatt cgctctggat cagctcgccc gccgtaaaat acgcaaggct tcccttgccc
     4741 cggccgtagg ccgcggggcc cagcgccagc tcgaactgct cggccacgtt ctccgtcagc
     4801 tcggtggcgg cggcctgggc atacgcgcgc gccgtcacaa cgatcagcac ctcgcccgcc
     4861 tgcccgtaca gattcaggct ggtgtcctgc atgtgggcca gctgctttgc tgtctgcttg
     4921 agcgcgttca tgtcgggttc aaacgtgcgc acgaccaatt gcttttccac attcttctcc
     4981 ataggttgca tgctgtgtcc tccttgccgc cccgccggga tctttgccgc cgggccgctt
     5041 ttttattttt tcacgccggc tccggcggtt ctggtatgct gcatgcgggc gctatacgtc
     5101 ccgcgcaggc gtgcctttct gcatcagatg ggccggctcc tccgccggga aggccgtgca
     5161 ggccggctgc ggcaggccca gcgccaggcg gcgcaccatc tccagcgcgt gctgcgtgct
     5221 ggacagacgc accacctcgc gcgtgcggcc cgtgataacg agcttctgta catagacggt
     5281 atcggcactg caagcggcca gatacacaag tcccacgggc ttttccggcg tgccgccgcc
     5341 cggccccgcg atgcctgtga tacccacgcc gaaatccgcc ccggcggctt tgcgcacgcc
     5401 ccgggccatc tcggcggctg tctcgggcga tacggcgccg tatgcctcca gcgtttcatg
     5461 ccgcacgccc agcatttttt ccttaatatc gtttgcatag gtgcacgcgc cgtaggcgaa
     5521 cacaccgctt gcgccgggca cggccgtgat gcgctgggac agcatgccgc ccgtgcagct
     5581 ctccgccgta gccaccgtaa ggccccgtgc ggccagctcc ttcaccacca cttcctccag
     5641 cgaatccgcg tccatggcat agacgcaatc gcccaggata tcgtaaaaat gcttcgcata
     5701 gtccaaacac atgcgccggg cctcgttctc cgacgcggcc ttggccgtga tgcggataac
     5761 gacctcggcc gtcttggcat agacggcggc cgttgggttg gcgttttcca gcagatggcc
     5821 gatcctgctt tccagatggc tctcgcccac gcctgtgacg cgcagcgtca ccgagtgcag
     5881 cacgctgtcg ctcatggcag ccagccaagg cttgacgctg ttcaggaaca tgggctgcag
     5941 ctccttcggc gggccgggca gcagcacggc atatttgccg ggcgcggcct ggtcctcgaa
     6001 gatcatgccc ggcgcagtgc cgttagcatt ggggatcttg cggccatggg tgggcaccat
     6061 ggcctgcttt ttgttgtttt caggcatcgt gcggccccat gccgtgaaaa acgcgcggat
     6121 cttctccagc tccgcttcat caaagcgcag cgtgtcgccg aaagcgcagg ccacggtctg
     6181 tttggtcagg tcgtcctccg tggggccgag cccgccggaa aacaccagca ggtcgctgcg
     6241 gccgcgggcc tggcgcacca cgctttccag ccgggcggga ttgtcgccca ccactgtctg
     6301 aaagtgcacg gtaatgccca gcgccgcaag ttcacggctc aaaaactgcg cgttcgtatt
     6361 caggatatcg cccagcagca gctcggtgcc gacggcaatg atctcagctt ccatattcgt
     6421 catcccctgg tttgcagaaa ataaagagac ccgctcacag gttcacatac accacttcag
     6481 cctgcgccgc ggcctgtgcc ttcagctcag ccagccccgg catgcccgcc tccgcggcct
     6541 cgatctcgtc cagatggggc ttcgttttcg ggtggggcgg cgtgaagatg gtgcagcagt
     6601 cctcataggg caggatgctc gtctcgtagg tgtcgatctt ccgtgcgatg tcggtgatct
     6661 ccgttttgtc catgccgatg cacgggcgca gcacaggcag gctctgggcc gcgtccgtgc
     6721 aggcgatggc cttcagcgtc tggctggcca cctgggccag gctctcgccc gtgacaatgg
     6781 cgtccgcgct ctctttttcg cacagaaccc tggtgatgcg catcatgctg cggcgcatca
     6841 gcaccgtgaa caggtcctcg ggagcgttgt cgcggatatg ctcctgcgcg gcggtgtagg
     6901 gcaccacata tacgttcagg ttgccggtat agggcgtgag ctttttggcc agcgtcagca
     6961 ccttcatgcg ggcgcgctcg ctggtatagg gcgggctggc gaaatgcacg caggccagcg
     7021 aaaggccgcg cttcgccatc atatacgcgg ccaccgggct gtcgatgccg ccggacagca
     7081 tcagcgccgc cttgccggag gtggacaccg gcaggccgcc cgcaccgggc tttttgccgc
     7141 cgtggacata cgccgcaaaa tcgcggatct ccaccgtcac attcacgtcc gggtggtgca
     7201 cgtccacctt gaggtgggga tacgccgaca gcagcacgcc gcccagctcc cgggcgatct
     7261 ccatactgtc catggggaac gtcttgtccg agcgcttggc gctcacctta aacgtctttg
     7321 ccgcccgcag cgcgccgcac aggtattcct tcgcggtgtc cgcaatgaca gcaaagtcct
     7381 tggggcacac ggctgcgcgg ctgatggccg cgatgccgaa aattttgccg atgcgctcct
     7441 ccgccgcgtc gatgtcctca tcgcccgcag gctccgcgta catcgtgctc tgcgccttgt
     7501 agattttaaa attccccagg gatttcaggc ggcggcgcat ggtcttgagc agcacgctct
     7561 cgaaggtggc gcggttgagc cccttcagcg acatttcgcc ctgataggcc agaatgatct
     7621 ctttcatatt ctgcaaaatt ccctttcgtt aaagtaaagg cggccgccct tacgggcgct
     7681 tccacaagaa aacaaagccg cgagtttctg ccgggcgggc gcgtccgccc ccccgtcagc
     7741 ggcgtatttt cgccaacgtg cgcatgccct cggccagcgc gtccagcagc gcacgcacat
     7801 cctccggtgt gctctcgccg gaaaacgaga cgcgcagtgc ggtgtccaca cggcttttcg
     7861 gcaggcccat ggccgtcagc gtatggctgg cctcgcctct gctgcatgcc gagccgctgg
     7921 atacatacac atcccggctc tcaaggaaat ggagcatcgt ctcgcttttg acgccctcca
     7981 ccgagaaatt cacaagttcg ggcagcgcgt ccgccgggct gttcagcaca atacccggca
     8041 ttccggccag cgcgtcccgc agcgttttat tcagcgccgc gatgcgcccg gcgcggcctc
     8101 cgtccgccag catccgttca atagccctgg ccatggcggc gatataggcc acgttttcgg
     8161 tgcccggacg acgcttctgt tcctgcccgc cgcccagaaa cacgggggcc agattgtagc
     8221 cgcggcgcac atacagcgcg cccacgccct tgggcgcgtg gatcttatgc ccggacaccg
     8281 cgtagctgtc gatggccgtt ccgttcagcc gctgcgggac cttgccccac gcctgtacgc
     8341 cgtcgatgtg tacggccgtg cggctgtttt tttccttcac cgctttggcc agcgcggcca
     8401 cgtccagaat ggaaccgacc tcattgttta catgcatggc tgtgacgagg gctgttttgg
     8461 agccgacggc ccgtaccagc gccccggcgt ccacatggcc ggaggcgtcc ggcttcacga
     8521 cggtgacgcg ccaaccctct ttttccagcg cggccacggg gttctgcacg ctggggtgtt
     8581 cgtagcccgt gacgacaatg tggtccgccc aggcggcgcg cgccttcacc gcgcccagaa
     8641 cagcaaggtt attgccctcg gaaccgcagg ccgtgaacac gatctccccc ggctgcgcgc
     8701 ccagggacgc ggccaccgtt tcccgcgcgc cggacaaaac gaactcgctc tgcgcgccgg
     8761 gcgcgtacag cgaggacggg ttggcgtaat gagcgcgcag caccgcgtcc gccgtgtcgg
     8821 ccacggcgtc ggccacgcgt gtggtggcgg cgttatccag ataatgcaaa ggcaaagcct
     8881 cccaaaaacc atatataaaa ttattatact accaaacaaa gtgcaaggca acggaaagaa
     8941 agaaaaaaca gcggcctgtg aggaaaagaa cacaggccgc cgtcatcggc atattgacac
     9001 cggagggcgc cgcctcttcc aaaaatcatc ccattgcatc gggcggcgtc cacatccgga
     9061 tatactgcag ctccccgctg ccggcaaaca cggtctccgg cgcggcgagg gcataagacc
     9121 ggcgcatccc gccccacggg tcctccattg ccgccaccgg caccagccat aaaccgccgt
     9181 tcgtccgggc cagcggcaag gccgccgttt ccccgcccgt atacggttct tcagcccgca
     9241 gcaaaacttc gcgcagctgc ccgtcccgca catcgatcaa ataggccggg acacagcccg
     9301 cgccgcttgt gctgaaagca cccgcccata aatacggttc ctgttcgccg cagcatgccg
     9361 gaagtatatc atgaagacca aagtccagcc atggtacgga ggccgacggc cggaacgaag
     9421 ccagcgcccg ctgcgtaccg ttaaaaatat cccgttccaa aagtgttttc agagaggcgt
     9481 ccccgtcatc atcctgaatg aacatctggt acagcttgcc gtcccgcaca tacatgccgc
     9541 caagatagcc gtagccatcg tactccccat accaatccat ctgcgggctg ccgaggcgca
     9601 tccgcgtgcc tgcggccaaa tcatactgca ccagccattc gtccatctgc tggccccgca
     9661 cctgctctgc ctcgccgaca gccacgccgg gataaacgcc gtcatccagc ggatattcat
     9721 aatgccgcat gaggaagctg ctgccggaca cgcccaatat actgtctccg cctgtctcaa
     9781 ataccgtata ttccccggtg gccaggtcta cccgcacgcc gccggagaca ttttcgctat
     9841 ggtcgtaata gcttccgtac agcgctccgc cgtccgtgta ggagaaagaa tcgctgtaca
     9901 cctcccagcc ctgggggaat tccatcaccc ttttgcgccg ggcgccggaa gcgtccacca
     9961 cgtcgatata agccgcggcc gccgatgagg ctgcgttttg tacaaaccac gtcccataca
    10021 cggagcccat caggtagacg ttcccgccaa tggccgcaac cgctacattg tttccctcta
    10081 tgtacgccgg gcacgcggcg tcactgtggc cgcagcccgg cacgctgcac agcggcgcct
    10141 gcgtcagcgt agcgtaatcc gtacgcaaaa gcatggatac gcgccgggta aggccgtccg
    10201 gcccggcggc gttctcggcc agcacagcca gttcgtaata agcgtcgccg ccgttgcttc
    10261 ccgttccgta gccgctccac tgcacggtgt ccagcgcacc tacggcgaac gccggggccg
    10321 gcggctcctg caccggttgg gaagagaccg gcgcgcagga ggaaggggcg ttgggggcgg
    10381 gcgcgcatcc tgccagcagc gccaggacaa ggcacagtgc aaaaatccgc ttcatcccat
    10441 cgcctcctct tccggctgct cctcctgcag ccgcgccgtg tccgcatccg cttgtccgaa
    10501 tgcccccgcg ccgaaggaac gctgatattc ctgccagccg cgcacggtgc tttcttccac
    10561 ggacgccgcg ctcaggctga aatacatacg ctgctggtcg cccccggctt caaatgtata
    10621 atcgggatat gcaacataaa acccgctctg gcacgcgagc agcagcccgg ccggggcgga
    10681 atacagaccg atctctgcat atttcgtacc ctccggcccg gcccagtctg ccaggctgtc
    10741 catgccgagg tatgttttgt aggccgcgag caccgccggc gcgtcgggct cctgcaggcc
    10801 cgcccactcc tctgccgtac cccagagccc caccaccttt tgggactggc tctccacagt
    10861 gacgccaatg atataatagc ccagattttc atcccgacgg ccatagctta tgtagttgac
    10921 aaagcccagg ctatcctcgc tctgatacgt ggacagtccg atctgatcca taaaataggc
    10981 ggcccactcc tccggcagca cgcccgccga cgccagttcc tccacatgga agagcaagcc
    11041 gtccgnnnnn nnnnnccggc tcgacataat acatggaatc cgggctggtt cccgcccgct
    11101 ggtaaccgct caggcttccg cgccggctgt attttttcag cacccgcgtt aaatacatat
    11161 cctcgcccgt aagcgccagc gcgcccgctt tctgcgtgcg cgcgtgcggc tgggccagca
    11221 gccccgcgtc gcccagccac agcgtcaggg cgggcacggc cgagagggcc agcgccaaaa
    11281 cgcccgtgca cagcaccgcg gccgcggggc gcgcgaacca tttcttccat ttgttcacac
    11341 tgccgctcct ccttctttgc cgtctgccgc gggcaggcgc accgtcaccg cggtgccctc
    11401 cccttcaacg ctttcaattt gcagccgccc gccgtgcagc gcggcgatct tctcgcacag
    11461 ggccagcccc atgccgctgc cgccgcccgc ccgcgcgcgg gatttatcca ccatgtaaaa
    11521 cggctccgtc acacgcgcca gcgccgcggc cgggataccg cagccggtgt cgcgcaccgt
    11581 cacgtccacg ccctggcctt ccgccgccgt gtgccagccg atggacacgc ggccgtcctt
    11641 gggcctggca cgggccgcgt tctgcacaag attgtaaagc aggtccacca gcagatcggc
    11701 gtcgccccgc acccgcacgc ccggcgcggg ctggaacacc ggctccaccg gcgcaacgga
    11761 cggccccagc gccgccctgg cgcgtgcgaa cacgcgctcc agttccagcg gctccagcgc
    11821 gggcgcctgc tcccgcaggc ccagcaattg cagcagcttt tccgaaagcg cctccacgcg
    11881 ccgcgcttca ctgaaaatat agttcgccgc ccgctgctgc tgctccggct cgcactgcat
    11941 gctgcgcagc gtgtccgcat aaccgatgac cgccgtcatc ggcgttttca gctcgtgcgt
    12001 gaacgcggac ataaagtcct cgcgctggcg caccgacagc tccagcgcat ctaccttttc
    12061 ctgcacggcg gccgccatag cgtcaaagct tcggctcagc acgccgatct cgtccccgcc
    12121 cgaaaggccc gtgcgttcgc cgtacgcgcc gcccgcaatg ctttcgctcg cctgtgtcag
    12181 acgcgccagc ggttttgtca gccagcggga cagcagcgcg atcatcgcgc ccgcgcacac
    12241 cagcaccacc agttccattt cccagaagcg cagcagcgca cggtcccgcg cggcgaacac
    12301 gctggtcacg tcgtgtcccg tcagcagcgt gacgggcgga ttgcccacgg tctccacctc
    12361 cgcctcgaac agcatatacg tcctgccgcc gtcccggtgc atgcggtaag tatccgtctg
    12421 gcccgtaggg ccctcgtccc agggaaacga ggtatacacc gtgcgcagcg ccgcgggctg
    12481 ccgctctgca tcggaagccg cctgtgcttc tgcttccgtt tccgtctccg ccgcggcgga
    12541 ggacgtcccg ccctgtgccg ccgcgtcacc gcggtacagg gccaccagct ttccgcccac
    12601 ataatcctcc agcgagctgc cgtagcgcgc cagatggctg tccgtcacgg tctcgccccg
    12661 cgcggccacg tccaacaggt cggattccag cgcatagcat tgcagcagat gctgcacctc
    12721 cgcctgctgg cccgcctcgt tcagagcatc gtcgaagttt ccggccagta ccaccgcgcc
    12781 ccccagcgag agcgccatgg ccagcaccag aagcatggcc agcgtcagct tctgggcaaa
    12841 gcccagtgca cggagcgtct ttccggcgcc gcctgccggg cgggccgcgc cgctttccgc
    12901 cgcctgcgcg cccgtccgcc tggcgcgggc gcgcttttct tttttcacgg ctctccctcc
    12961 atttccagaa gataccccac cttatagacg gtcttgatat gctcctgcca acccagcttt
    13021 ttgcgcaggc gctggatgtg caggtccagc gtgcgcgtgc cgtcatccat ctcgccgccc
    13081 caaacacgct cgaacagcac gtcgcggtac agtgccgcgc cccggttgcg caaaagctgt
    13141 tccagaaggt cgaactcccg gggcgtgagg gccaccggca cgcccgcctt ggtggcgctg
    13201 cgcgccacgg tgtccaccgt aacgtcccac gcgcgcagca gcgcgccgcc gcgcccggta
    13261 cggcgcagca cactttccac ccgcgccacc agttccgcca cctcaaacgg cttcacgatg
    13321 taatcatccg cgcccaggtt cagcccgcgc acccggtcgc ccagagtgcc cttagccgtg
    13381 atgaaaatga caggcgtgcc catgggccgc agatactcca gcagctcata gccgtccagc
    13441 ccgggcagca tgatatccag cagcaccagg tcgtaatcgt tctgttcgat cttatccgcc
    13501 gccgcgcagc cgtcgttgac ggcctcgcat tgataacctg cccgcgccag gctcatttca
    13561 atgagctggg cgattggtac ttcgtcttct acgatcagga tgcggttcat gccgcttccc
    13621 cccttgcatc agtttctacc atcagagtaa cggatgaatg tatcagattt gtttcattcc
    13681 cgggcttata cgcaaaaaac aaccgtcggg aacgggcata cgcccgcctc ccggcggctg
    13741 ttgtttcaca tttttcagta agccagcacg atgccgccgt cgtaagcgta taccgtggac
    13801 aggccgctgg tgggcaccag gatcacctcg cgcagtgccg cacatttttc cagaaattcc
    13861 tttttcagca tcatggcgcg ctcggggcag ttgcagtagc tcagcaccat cgtcaggctg
    13921 gaggctgcgg cctcccgcgt gccttcggcg atgagctcca ccagacggcg catggcgttc
    13981 tgggtgccgc gcaccttttc gatggccgcg atgctgccgt ggccgtcgtc gcccatgatg
    14041 ggccgcagcg aaagcatgcc gctcaaaagg cccgccacct tggagatgcg gccgtttttc
    14101 acaaggttgg aaaggtcctc cagcacgaag cgcgttttca tccgtgcaat gaacgctgtc
    14161 atctcggcga cgatgtcctc gaaggaatgc cctccgtcga tgcggtcgcg cagcatcagg
    14221 gcgatgagcg tttcgcctgc ggacgcgctc tcggagtcaa aaatatgaat cttcttttcc
    14281 gggctgcgct ccagcaccat ctcccggcca acgcaggcgg cgttatagct accggacagc
    14341 ttgccggaga gcgtcaccac aaagctctcc tcgctttttt ccatcagcgc ggcgtattca
    14401 tccggcgagg ggcaggctgt ggagggagcg tttttatacg ccttcatgtc cgcgatgagc
    14461 ttttttgtgt cgatgcgctc gtcgtccaca tagtggatgc tccccacgtc gatcttcagg
    14521 cttgcgacag acacgcgcag caggttgcgc agcgcggggg taaggtcgca gcagctgtct
    14581 acaatgatct ggatatccat tcctgttctc cctctactct cacaatatat accattctaa
    14641 cttttacggg gcaaaaagtc aattgaaagg cctcttccct tgtaatcata tcagaaattt
    14701 gttaaaattt tatgcgaacg gcgtgtatat tttacgcgcg ccgcaaaaac gggagtgaaa
    14761 aagggagcgt ttgcatgaaa accgatatcc agatcgcgca ggaagccgtt ttggaaccca
    14821 tttccgacgt ggccgcgtcg ctggggctgg acggtgagag cctgatcccc tacggaaagt
    14881 acaaggccaa ggtggaccac cgccttgtga aaaagctggc cgaaaaaaag gacgccaaag
    14941 tcattcttgt aacggccatc agccccacgc ccgcgggcga gggcaaaacc accaccagcg
    15001 tgggcctggc ggacgcgctg caccgaatgg gcaaaaacgt cgtcctcgcg ctgcgggagc
    15061 ccagcctcgg ccccgtgttc ggcgtaaagg gcggcgccgc aggcggcggg tatgcacagg
    15121 tggtgcccat ggaggacatc aacctgcact ttacgggcga tcttcacgcc atcggcgcgg
    15181 ccaacaacct gctggccgcc atggtggaca accatattta tcagggcaac gcgctgaaca
    15241 tcgatccgcg gcgcatcacc tggaagcgct gcgtggacat gaacgaccgc cagctccgct
    15301 tcatcaccga cggcctgggc ggcaaggcca acggcacccc gcgtgaggac ggcttcgaca
    15361 tcaccgttgc aagcgaggtg atggccattt tctgcctggc caccgatctg gccgacctga
    15421 aggcccgcct gggccgcatc gtagtgggct acacgtatgc gggcgcgccc gnnnnnnnnn
    15481 ngagcagatc ggcgcggcgg gcgccatggc cgcgctgctg aaggacgctt ttgacccgaa
    15541 tctggtgcag acgctggagc acacgcccgc gctggtgcac ggcgggccgt tcgccaacat
    15601 cgcccacggc tgcaacagcg tcatcgccac aaagctgggc atgaagctgg cggattatgt
    15661 cgtcaccgag gcaggcttcg gcgcggacct gggcgcggaa aaattcctcg acatcaagtg
    15721 ccgcctgtcc ggcatcgagc cctccgccgt tgtggtcgtt gccacggtgc gcgcgctcaa
    15781 gcaccacggc ggctgcccca aggcagagct gggcgtgccc agccttgaat atctggaggc
    15841 cggcagcgcg aacctggccc gccatgtgga aaacatgcag agcaaattcg gcatgccggt
    15901 gtgcgtggcc atcaacgcct tccccaccga cagccccgag gagcaccagt acctgcggga
    15961 cctgtgcgcc cggctgggcg tgccctgcgc gctgagcgag gtgtttgcaa aaggcggagc
    16021 gggcggcctg gagctggccg aggatgtgct gggcattttg gaaaaacgtc cggtgcgctt
    16081 cacctatccg gacgacgcgc ccgtggcgga caaggtccgc gcggtatgca aaaacatata
    16141 cggcgcggcg gatgtggcgt tttccgccgc cgcaaaaaag cagcttgcag agctggacgc
    16201 gggcggcttt ggccgcctgc ccgtgtgcat cgccaagacg cagtacagct tctcggacga
    16261 cgcctcgctg ctggccgcgc cctccggctt cacgatgacc gtgcgggagc tgcgcctttc
    16321 tgcgggcgcg ggcttcgtcg ttgtggtgat gggcaatatc atgacgatgc ccggccttcc
    16381 caaaaagccc gcggccgagg gcatcgacgt ggacgaaaac ggcgtcatca gcggcctgtt
    16441 ctgagcgctt tctggcaaac gccgcacaaa aaagcatatg caaacagcgc cgctccaaag
    16501 gcacatcttt ggagcggcgc tgtttttttg cacttgtttt ccgggcgctt tacagcacct
    16561 taccgagaaa atccttcaaa cgcggatttt tggggcttcc gaagaactcc gccggggcgg
    16621 cctcctccat gataacgccc tcgtcgatga acaccacgcg gttcgccacc tcgcgggcaa
    16681 agcccatctc gtgggtgacg accaccatgg tcatgccctc ccgggcaagc tgctggataa
    16741 cgtccagcac ctcgtgtacc atctccgggt ccagcgcgct ggtgggctcg tcgaacagca
    16801 tcacgtccgg ctgcatggcc agcgcgcggg caatggccac gcgctgctgc tggccgccgg
    16861 agagctggat gggatacgcg ccgccgcggt cgcccaggcc cacgcgctcc agcaagctct
    16921 gggccagctt gtgggcctcg tccctcttca tcccctttac cagcaccggc cccgcgctca
    16981 cgttgtccag caccgtcatg ttggtgaaca gattgaactg ctggaacacc atgccgatct
    17041 tctggcgcat ctggtcgatc tttgtctcct tggaggtgat gtccacgccc tcaaacacga
    17101 tctgtccgcc cgtgggctcc tccaacaggt tcaggcagcg caaaaacgtg gatttgcccg
    17161 agccggacgg cccgatgacc gccaccacgt cgcccttgca gatgtcgatg ttgataccgc
    17221 gcagcacctc cacatgcttg aagtgtttct ccaggccctt gacctcaatg agtttttctc
    17281 ctttagcggg tgacactctt gctcatcctc ctttccacag ccgcgatgat cttcgacagc
    17341 gggaacacaa tgataaaata catgatggcc gcgatgacat acggctccag gaaaatatag
    17401 gtctgcttgg cgatggactg ggcgcggaac atcagctcta cgatgcccac catgcccagg
    17461 atactggtct ccttgatgat ggtgacgaac tcgttgcaca tggcgggcag gatgttgcgc
    17521 accgcctggg gcaggatcac atggcgcatg gccttccaat ggctcatgcc gatgcagcgc
    17581 gcggcctcca tctggccgcc gggcaccgcg ccgatgccgg agcggatgac ctccgacata
    17641 taagccgcgg agttgaggcc gacggccacc atgccccaga acatgcggtt gaggctggaa
    17701 tccggcagct tgaaaacatt gcccagaccg tagtagatga tgaacacctg caccagcatc
    17761 ggcgtggcac ggaacacctc cacatatgcg gtacaaaaaa atttggcaac agccttgagc
    17821 gccttgactg ccagattgtc gcgggactgt acgctggcca tacggccgga cgccagcacg
    17881 agccccagca caaggcccat cagcacgctc acaaatgcga acagcagcgt atagccaagg
    17941 ccgttccagt acatatggcc gtacttttcc catattttca gcattcgctc aaatgaaaac
    18001 atcgtatcct ccttcgtggt aaaacggcat tctccgcccg cggggcggca gaaacacaaa
    18061 gaccgcggcg gagggacgcg ccctccgccg cgtatcaaat tcaatgcgat gtcctgcagc
    18121 ttactcgatg cccatggaag cagccttctc ctgcgcttcc tggaaccact gctccagcag
    18181 gccctcggcc tcatatgccg cgatgcgctc gttcacccaa tccagcagat cggtgttgcc
    18241 cttcatcaca gccacagcgt tgccgccctc ttcgggatcg atctccacag gcaccttgcc
    18301 gatggccagg ccctcgactg tctgcatata ctgctcggcg ctgatttcgt cgatcatcac
    18361 agccgcgcac tcaccgttct tcaggttggc gatgcactgg gggaagtccg gcaaaagcag
    18421 gggctctgca tcggggaact gctccgccat cagcgtctcc tgcagggcgg ctttctgagt
    18481 ggccatcacc gcgccggcca ggctttcctt ggtggcatat ttgtccaggt tttcctccag
    18541 caccagcatg cactggccgc cggcatagta catatcgctc atgtccacgc tctttttgcg
    18601 gtctgcgttg ggcgtaaggc ccgcgatggc catatcggcg ttgcccgcct ggacctcagc
    18661 cacaacggaa tcgaacgcga tgttctgtat atccagttca acgcccagat catctgccag
    18721 cgcctgcgcc agggaagcgt ccaggcccac gacgttgtcg ttctcgtcca ggaactcaaa
    18781 cggcgcatag ccggcctctg tcgttacaac gagcacgccc ttggccttga tggcctccac
    18841 gctgtgctcc tccacattgc cgcccgtcat tgcgatgccg tcgtcggctt cggaagcgga
    18901 cgtgccggac gcggccggag cgcttaccgc gccggacgaa gccgcgggag cggagctgga
    18961 agagccggaa ccgccgcagg cggcgaggcc cagcgccatt acaagcgcca tgcatactgc
    19021 tacaagcttt ttcataagta ccattctcct ctttcaacaa gtgcatattt acaggcgggc
    19081 gcttcgcagc ggcgaccgct gcggccggcc tgttgtctga gtcttagtat acatgcataa
    19141 atatgcaaag tcaagtgaat attttgcata ttatttattt tcccattttc aacagaaagc
    19201 gcgttcctcc cttgcgtttt gcagtcgttt tgcacaattg caaatgcctt tcccggcgcg
    19261 gcgggcggga acgcagcgtc ggttccagtg tttctcccgc aaatttgggg cttcggcact
    19321 ggataccgca caaaaatatg ttatgataat ggatacggat ttcacgaaag gaagcgtacc
    19381 ccatgcatct ctctcccgcc cgcattttga actttatccg cagggaggcc gttctctgct
    19441 gcgcggcgct ggccgcgctg gcctccactc tttttgtgcc gccctccgcg gcgtatattt
    19501 cctatttcga cttccgcgtg ctgtgcctgc ttttctgcct gatggccgtc gtggccgggc
    19561 tgcgtggctg cggtctgttc gaggcgctgg cgctgcgcct gcttcgcggt aaaaagacac
    19621 tgcgttttct ggcgctcatg ctggtgctgc tgcctttttt cagttccatg ctcgtcacca
    19681 atgatgtggc cctgctcacc ttcgtcccct ttgccatctt gattctgaac atggccgatg
    19741 cttccgccta tgctccgtac atcatcgtac tgcagaccgt cgcggccaat ctcggcagca
    19801 tggccacgcc tgttggcaat ccgcagaatc tttttctcta caatcgtttt gccctgtccc
    19861 ccgccgattt tttccgcacc atgctgcccc ttgcggcggc cagccttgtg ctgctgtgca
    19921 tgggcgtgct gttcgtgccg ccccgggccg tcaccgtcgc catgggagat gtgcacggat
    19981 acaacacgcg ccgcctgccc gcgctgagcg tactgttctt gctgtgcctg ctgtgcgtat
    20041 tccgtgtggc accataccag cttctgacgg ctgtggtact ggccatgctt acgctgttca
    20101 tgcgggacct ttttcgggaa gtggattaca gcctgctgct cacatttgta tttttcttta
    20161 ttttcgcggg caacatggga aagatcggcg gggtgaacgc gcttctttcc gcacttatgg
    20221 acaagcacgc gctgcccacc tgtgtgttgg ccagccaggt cgtcagcaac gtgcccgccg
    20281 ccgtccttct tgcgggcttt tcacaggact ggcgcgccct gctgacgggc acgaaccttg
    20341 gcggcctcgg cacgcccatt gcctctctcg caagcctgat ctccctcaag ctttatgccc
    20401 gctccgcgcc gggcggcgca cggcgctatc tgctcgtctt ttccgcagtc aatgttgccg
    20461 cactggcgct tctgtacgcc cttgcggcgg ccggctgacc ggctgcggcg cttttgcctt
    20521 tctgcagaag cgaaataccg ccccacgccc cgccgtccgc gagtgcggca gaacataaaa
    20581 cagcggccgc cccttgcggg gcggccgctg ccttttgtgc tgtattttgc cgggacgctt
    20641 acgcctggct ggcagccacg gcgcaggcca cggtggcgcc caccatgggg t
//
LOCUS       DAWBSB010000010        20036 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_723_length_20036_cov_3.514539, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000010 DAWBSB010000000
VERSION     DAWBSB010000010.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 20036)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 20036)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..20036
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_723_length_20036_cov_3.514539"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    9266..9275
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4032 a         5805 c         6125 g         4064 t
ORIGIN      
        1 agcaaagagc atcagaaacg gttgaagaaa aaataatcgt agactgaaca cccctttcca
       61 ttcaatcggg cctcctcttc gcacaacagc accggcgaaa gcgcaggacg tcccgcagaa
      121 aatgttcgga aagttttcaa catacggccg ctgctgtgtg gaaaacactt cgcctaaaag
      181 aatttttttc gatctttgcg ctccggtata ccgggcagaa aaaacgtcaa aaaatcgaac
      241 aagaataaaa atatattgat aaacaataaa aaatacttgt atttcagaaa atactatgat
      301 atactgaccc caacgacaat cggtggagga atcgttatga actggaacaa agacaaaagt
      361 gtcaagcttt ccaaattctg cgtctgcctt tttatgctca ttctggcagg agtttgcatc
      421 ggtgcaccat ggttgttccg gctgctcatc cgtctgcgcg tgcaggattt ggccggaaaa
      481 ttcgagttgt tcctcgtcag cacctatacg gcggccgtgc ccgccgctgc ggcactgtac
      541 ggcctgtggc gtttgctgcg caacatcagc gagggcgagg tttttatccc ggagaacgtc
      601 tccatcctgc gcctgttgtc ctggtgctgc atcgcggccg ggctggtatg tctgttcagt
      661 gcgctgtact atatgccgtt cctcatcgtc agcgcggctg cggcatttgt tggcctgatt
      721 ttgcgcgtgg tgaagaatgt ctttgccgag gcggttcggc tcaaagatga aaacgactat
      781 acgatttgag aaaggacgtg ccgaaatgcc cattgtggtg aacatcgacg tgatgatggc
      841 gcgccggaaa atgagtgcgg gagagttggc cgaacgcgtc ggcatcacgc ccgcgaatct
      901 ctccatcctc aaaaacaata aggcgaaggc cattcgtttt tccacgctgg aggcgctgtg
      961 caaggcgttg gactgcaagc cggcggacat tttagactac acggaggact gacaaaatgg
     1021 aacaggagaa aaagacggcg ggcatgccgt gcccgccttg taaaagtgcg ccgttttcaa
     1081 gggccgtgaa gcccgcggcg caggagaatg ccgtgcagga ggccgccgca ccgcttggtg
     1141 cgcccggtca gtacgggggc tccgccgtcg gccggacaga aaagacgccc ggcttcaccg
     1201 gagtggacgc ggcgtttgcc tgtggtttcc tggccgtggg ctggctgttc tgggagcttc
     1261 agctctggag cggctggcgg tggttcggca atgcgggcac gggcaccgct ctgttcacgg
     1321 cactttttgc gggcgcggta ctggcttatg tgtatcttgc aaagctgcgc ccgccccggg
     1381 aaagctggtt ctggctggcg gtactgcttt gcctggggct tggctatgca ctgccctacg
     1441 gcggcggact gttgggagcc gtccattacg gcgcgctgct gttcacagcg gaatactgga
     1501 cgctgagcgc cacgggccgt ctgttgaaaa gtggaaagac ctctaattgg ctgccgctgg
     1561 acgtgctcaa cgctctgttt gttctgccct ggggcaactt catgcgtctg cctgcggcgt
     1621 ttgtttttgg cttgaaaggg cttggccgcc gtgtgcgcgc ggcccgttct gagccgggag
     1681 agcgaagccg gggcaaacgc ctgctggggg tgctgggcgg cgtggcgctg gcgctgctgt
     1741 gcctgtgctt cgtgctgccg ctgctgatgg cggcagatga cggctttgca tcgctgcttt
     1801 cgggttttgt aggccgcctt gccgcatggt tttccggctg gagggattcc tggacggagt
     1861 ttgtcgtgaa agtatttttt gcgctgccca cggctctgta cctgtatggc cttgtatatg
     1921 gcgccgtgcg cggccgccgc gcctgtgttt acgacaaaaa ggaggtgtgc gcggtgcagc
     1981 ggggcctgcg gttcgcgccc cgctccacag tccttacggc tttgttcgtg ctgtgcgcgg
     2041 tgtatctgtt gtttatctcc atgcaggcga aatatctgtt cggcgcattt tggggcgcac
     2101 tgcctgaggg attttcttat tccgagtatg cgcgtcaggg cttttttgaa ttgtgccgtg
     2161 tggcggccat caacatcgtt attctgctgg ccgccaatgt gatgagccgt gcgcaggctg
     2221 cggaaaactg tctgctgcgg gtatgcaata ccgcgctttc ggcgctgacg ctgctgctgc
     2281 tggccacggc agctgcaaaa atggcgcttt atatcgcggc ttacggcctg acagtgaagc
     2341 gcgtgcttgc aagcgtgttt ctggtctgga tggcctgcgt attcgtgtgc gtcatcctgc
     2401 ggcagttccg cactgtggcg ttggtgcgcg tcgcggtgtt tctgggtgca gtgctgtttg
     2461 cgctgttgtg tgtgctgccg gtgggggacg gcatcagcac ttataatgcc gcccgtgtac
     2521 aggcgggcac gctggacccg gacgtgatgg agcaggagct gcgccccctc gtgtaacaaa
     2581 ataccgctcc gaaaagagca gttttgcgca tggcatcgtc aagcacgcct gcggaccgaa
     2641 atcatggccc acattttgcc gccgggacat gccccatcgg gtttggatgt acaatgcgcg
     2701 aaaaaggggg aaagaaatat gaggaaaaag ctttgctctg cggctgtttg ctgcctggtg
     2761 ctttttctta cggcctgcgg gcttgcttca caggcgtcgg tggcggctct ggtggagcgg
     2821 gacgtacagg cgctggaggc gctggcgggt gagatcgctc tggcaggcgc cgccggtgac
     2881 gcagaatatc cgggagtgga ccgcatctct tatgacagcc gtaccggaca ggtacagttt
     2941 gaatgcgggg tctcgggctt tgcatcccag acaagctata atggtttcta ttattcgccc
     3001 ggggatgttc cgctgggctt cggcggcaca ggggatatga cgctcgctcc gtcgggcgcg
     3061 ggctggtgct gggaagaaac ggagggggac aactggtatt acaccgagag gctgcgcagc
     3121 ggctggtatt actacgaaat gcatttctga cggccgcgcg gcggccgcac acttgacgca
     3181 ggcgcgcttt ttgcgtacaa tttcctttgg catttcattg aatgacaaag gagaggaaac
     3241 gacatgaagg cacgcattcc caaacaccgc gaattcatca tcaacctcgc ggaggattac
     3301 gaaaagaaag acgaatgctg ggacaagctg caggagatca tggaggcgta taagaaagag
     3361 ggaaaatccg tttatacgcc cacgttcatc gaagataacg aggataaggt gaaagcgctg
     3421 caggagcagt acgagttcac ttataccatt gaggagcggt gacggtgcaa tctttcctgc
     3481 ggcgcgggaa aggagcacac aatggaattt tttgcaggga tcggcgcggg ctggatgaac
     3541 cttgccagcc tggctttggg ccttttggcc tggatgctgc ccgtggcggc gatgatccgc
     3601 acggcggccc ggccgcgcgc gctgcatccg gcgctgcctt tggtatgcgt cagctttgcc
     3661 gcctgtgcgg cggcgctgta tcttcaattg cgttatcatg cgcatctttc gcgcattgag
     3721 gatgtgggag cttttttgga tacggcgcag gcggctgcgt tcgcatcggc cgtgctgctg
     3781 tgcgtgacgc tggcgctgaa cgcccttgcg ctgtgcgcgg cggccgtttg cgcgcggaaa
     3841 gcggcggacc gggcgtaagc agcccaaaat aactgtaaaa aaagaatgcc cggccccgta
     3901 aagcagcacg gggccgggca ttcttcatta ttccgcaata aaaatttctc cgaatgaccc
     3961 tttaaaaatt tagtgaagca gggttgaata ttttatgaga aggaacagag gaaatactgt
     4021 gttaagcctg ttttcaacga tacatggcct gagagccata ttttccgggc cgcggaaagc
     4081 gcaggctttg cggtgcggag tcaaaggctt atttcagaat ttcccgctgc tcggccagaa
     4141 gctgttcctg atatgcaccc gcaaaagtgc ccgccaggaa acggggcagc gcttcggaga
     4201 caaggcgctc ccagctcgtt ttttccttgc ctacgagaat gggatagaac agcgcgtctg
     4261 cgtgctgcgc ggcggcaagg tcgcccaggg cgtcgccaac catcagcaca tggcccggcg
     4321 catagccctt ggcagaaagc gccgaaaggc aggcctcctt ggtgcctgcg tcctggccca
     4381 gaacgacgtc catatgctgg acaaggccgt ggcgcgtcca ttcttccgcc aaagcctcgc
     4441 tgttggcgct ggaaacaacg gcggtgtccg tcagcacatg gatcgccgcc agggcctctc
     4501 tgcagccggg aaacggccgc gaatcatcgc ccatggcggc gatggccgcg ttgacggcgc
     4561 ggctccattc caacgctttt tgcaggcccg cgccgccgcc ggccgcaacg gcttcctcca
     4621 gcgccgggtt tgaaagcttg ggggcctttt ccacccaggt gcacagctcg tccgcgccgg
     4681 ggacacgcac gccgtgttcc gccagcatgc gcatcgtggc ggcaagccct ttaaagcggt
     4741 tcacgccgcg ggtgcggctg tacaggttca cttccatcca gtggggggtg atgagcccgg
     4801 cgtattcctc cagcccgtac acacggatga gctgtgggca gaaggcctga tggtgcttgg
     4861 aatccatggt gtccatggcg cagccgtcgg aatccacgca cacaagccat tcttttttgg
     4921 gagtgaacgc ggagaacgga ttttcagaca taagcttcct ccttggattt gaaaggttca
     4981 tatggttgca ggggccggaa agcaggaaag gcgcgcgggc cgcattatgc ccgcgcgctt
     5041 ttttgcaaaa gattcacaga tgcgtgtccc acatgccgca gaagctgtcg atggggtctt
     5101 tgcccttgaa agcttccggc ccggccatca gctcgtccac cagcacatcc agcgtagcgg
     5161 gagcggaatc gtaggtgttg atataggtgc gggcctgggg aatgtccgcc aggatcgtcg
     5221 gctgctggca gccgatgacg ataacaggga gctcgaacac ataccagggg atctcgccgc
     5281 cgccctttgt catgccgaag cccgggcgcg ccacagcctg gaagccgccg gccacgtcca
     5341 caagtgtgat gataagatcc tgtgcgccca caaaatccgc aatggtatcc ttgcctgcga
     5401 agtagatgtt gaaatccact ttttcgcccg cggcgatgcg cttttggatc ttgtccatcg
     5461 ggctttcgta cagcgttact tcaaagccct tggcttccag cttttctttc aggctctcgg
     5521 ctgcgttttt gccgccgccc atcagctttt tcatcgcgcc gcccaggccc ttggcctcca
     5581 gcccggtgac gtacacgatc atgatgcgcc tgtacttttc cggcgtcatg ggcagcacgt
     5641 ccgcgtcctt gtatttcacc agcgtcagcg catgctcgga gatttctttc tgcatggccc
     5701 tgtgcgcggc gcagccgata accctttcca tctcggcggc ggggggaaca agctctgtcc
     5761 gtgccttttt gtgcagaccc atgtgggctt tcagcgccag gatgcggtgc agggcgtcgt
     5821 ccaggcgttc gtcggtgatg acgccgtttt tgtaaccgtt catcatggag gcgaagtctt
     5881 cgtccatgtc gttgaagaac aggaacatgt cgcagcctgc ggcaatggcg gcggggatca
     5941 tgtcgctgcg cttcatgcgg caggtgaggc ccaccatatg gctggcgtct gtgagcacca
     6001 tgccgttgaa gcccattttg cccttgagca ggtccgtcag caactcctta gacagcgtgg
     6061 ccggcatgat gtcgtcgtcc ttgatgtccg ggttcatggc acgggtatag cttggaagca
     6121 tgatatgtcc ggccatgatg gcctccagcc cgttgtcgat gaggtttgcg tagattttgc
     6181 cgaaggtggc gtcccattcc tcgcaggaaa aggtgttgac gctgttggca acatgctggt
     6241 cacggtcatc caggccgtcg ccggggaagt gcttggccgc gcagcagaag ccgggcacct
     6301 cgtgcgcgcc ctccatgtag gcctgggtca tttccatcac gcgctgcgga tcgttgccgt
     6361 aggtgcgcag gccgacgacg gggttgtgcc agttgtagta aatatcggaa accggagcga
     6421 agctgaggtt gcagccgatg gccgcggcct ccgtgttggc cattttgcca agagctttgg
     6481 cgtattttgc gtcgcccgtc gcgccgatct ttgtctgtgc gccgatcatt gtgccgttgc
     6541 tgcacgcacc gttgccgccg ttctcggtat tgcaggcgat gatgagagga attttggaat
     6601 tttcctgcag actacggttc tgctcatgca cctcggccgc ggtgccgggg ttgtagcgga
     6661 taccgccgat gtggtatttg tcaacggtca ttttcaaata ttcctcggtg cgggaggaac
     6721 ccatgttgaa aaacagctgg ccgatctttt cctcaagcga catcgaggcg atggtgtcct
     6781 ccacccactg cacagcctcg tcatccaggt aatagggctt tgcacgcata tccacgggca
     6841 ttcgatcaga tcctttcttt ttgttttgtt ccgattgatt attttgtgca gctttgcacc
     6901 aaaagaagag tgattgcgaa ttgttaaaaa ataacctgca aaatctttcg attttattta
     6961 aaattaacaa agaaaatcgg ataagtcaat gacatatctt gcgaaactgt gctatactga
     7021 aaccacgaaa tacaatatcg ggaggaactg tgcatggaag tgctggagcg tctgacccct
     7081 tatgtggagc ttgtgcatct tctgctcaat acaaatttta cttatctgac gcgcaagctg
     7141 gcgccaagcg attcgctgga ccgcgcgcgc atcccgtccg ccgtggcaaa aagctacgac
     7201 gaggtgctgt tcggcggcgt cctgttcgac tgtgttcccg gcatcccgtg cgagatcaaa
     7261 acccctttcg gcatatgctt cacggcgctg gcgctttgga aaggggacgg cttcgtgctc
     7321 atcggtcctt atctgcccga gcgggcggac ggcgcgcagc tggaggagat cctggcgtcc
     7381 cagggcgtgc cgctgggcct gaaagaacag tacggcgttt attacagaga ccttcccacg
     7441 ctgagccgcg gcaaggtgcg cgccgcactg ggcgtactgg cggcgggcgt gtacggcgag
     7501 agccttgcgt ccagcgcttt cagcagccgg gatatctccc cggaggaccc gtccccctgt
     7561 cccgtatttg aggaggattc catgcaggtg cgcgcagacg ccattgcacg gcggtatgag
     7621 ggagagcgca ggcttatgga agcggtggcc cgcggcgata tgcgcgccgc cgacatggtg
     7681 gaggagacaa ctctgcggct ggagcgggtg cccaataagc tgcgcaaccg gaaaaacctg
     7741 ttcatcgtgc tcaatacgct gatgcgcaag gctgtggagg ccgcgcaggt gcatccgttt
     7801 tatatcgacg caatcagcgc caaatgggcc atgcgcatcg aggcggtgga gcaggaggcc
     7861 gacctttatc ccatgcggcg ggagatcgtg gaggattact gtcgtctggc gcagacgcgt
     7921 tccatggcca gctattcccc caatgtgcga agcatgctca cttatgtgca attcaatttg
     7981 gccgagggca tttcgctgga ggccattgcc cggcagctgg gtgtgaacgc cagctatctt
     8041 tccagccaat tcaaccgcga ggtgggcaaa agcctgccgg aatatgtttc ggaaaagcgc
     8101 attgcggagg ccaagcgcct gctgcgcggg cgcgcgcagc tgcccattgg gcagattgcc
     8161 gcggcggtgg gcttttcaga cgtgaactat tttacgaagg tgttcaagaa aaaagcgggg
     8221 cagacgccca gcgcctaccg cgcggcacag cgcgcgctgg cggcggaaca gaaagagata
     8281 tgaatatgcg gcggccggaa cccggccgcc gcatattttt cttgagtaag gaaaccaccg
     8341 gcagatgact tattcctgtt ctcgtccgct ccgccgcggc ttgcgcgctt tgagaatgag
     8401 catcatcggc cgccgcagct cgtcggccat accgggcacg gcgtccagca tctcccgcgc
     8461 aggctgcggc tccaccagcg cctccagcgc gaagccacgc gccagaagcg gctgcacata
     8521 gcccgtaacg gtgcggtggt atttgcgcat atgctcgcct aaaaacaccg cgtcccgggg
     8581 tccctgctca aagtatcggt ccacgggcca gtgcagcggg gcgccgcctg cgtccctctg
     8641 ccagtcctgc ggcccctggg ccgtgaacac cggatgttcc gccgaaaaaa taaaccatcc
     8701 gccgggggac agcgcgtccc agaccatgcg gcacacgccc gcgtaatctt ccacataatg
     8761 cagcgccagt gaactgagaa cgacgtccca agccgcaggg gcgggccgca tatcctcaat
     8821 ggccgcgcgc cggtattcca cgacggggct gtcggtcatg gcgcgggcgc gttccagcat
     8881 gcgttgggaa aggtccgtcc caagcacacg ggccgcgcca tgctcggccg cgtaccggca
     8941 atgccatccg aaaccacagc caaggtccag cacgcgcttg ccggcgagct ccggcagcag
     9001 cgctttcagt gccggccatt cgcctgcggc ggaaagcccc tgctcggaac ggggcatatg
     9061 gctgtactgc tcgaaaaata cgctgtcatc gtatttgttt tctttcaaaa aagcacttcc
     9121 cctgcatgtt ttcttcatgg taacacatct gttctgcggc ggcaagcctg cgcggcaggc
     9181 cttttttgtg caggtgaggg cctgttttgc gcagcttgct ccggagcgct tgcagcggcg
     9241 ggccggggtg tgtatcataa agatgnnnnn nnnnnccgcg ggataaacac aaacaaaggg
     9301 ggaggggctg ttgaacatcc gtatctgtga ggacccggcc tgcgcggaca tcgaggtggt
     9361 ggtgcgctgc aggcagcttg acgacacggt gctttcactt ttggcgcgcc tgcgcgttta
     9421 cgacgcgaag ctcaccggag aaagggacgg cgccacctac gttctggcgg cggcagacgt
     9481 gctgtatgcc gacacggcgg acaaaaagac gtttctttat acagcggacg ccgtatatga
     9541 aacgcccctg cgcctgtacg aactggagga gcagcttgcc gcgcgggatt ttgtgcgcgc
     9601 gggtaaatcg gtgctgctga attttgccca tgtggcggcg ctgcgtccgg acttcggcgg
     9661 gcgaatgcgg gtgacgatgt ccaacggcga ggtggtggtg gcgtcgcgcc agtatgtgcc
     9721 tgccctcaag gcaaagctgg ggctgtgagc cctataccgg aaaaggagtg tttgctgtga
     9781 aaaaattttt tgatatcgta atgcctgtaa agacggccgc atgctatatt tttacggcca
     9841 ccatgtgcgt gtatgccgtt ctggatctgg tctttggctg gggcggtctg cccgataact
     9901 acctgttttc gtttctgctg ctgtcgctgg cgggcggcgt gctgcagatc gtcgcgttta
     9961 cagagcttgt tatcaaaagg ctgcgctata cgctgcggct gttcgtattt gcagtgccgt
    10021 atctggcggt gctttcggcc attgccgccg ggttcggctg gctgcccgca gcgcagccgg
    10081 gtgcatgggc gctgtttttc ctgattttcc tggcggtgct tctgacaatg acgctggggt
    10141 tcgaattgta ttaccgcatc agcggtaaaa aatacgacgg cctgctgggc cagtaccgcg
    10201 cttcccgtac acgggatgaa aacagccggg gagaatactg atgcatgcgg cggaaagcca
    10261 gatgaaagga atgaaaagtt ttcaacaaaa ggggcctgcg cggtggaaaa ccgcacaggc
    10321 cctttccgtt tatcataaag ctgcagcctc agctgttttt caggatgacg cgctccacaa
    10381 catcggccac atcctcgcat ttgtcgcagc atttttccag gcggtcgaac agcgccgtcc
    10441 aagccatcac gtccacgggc gtgctggcat ccgcgtacag cgtgtgcatg gcgtctgtgt
    10501 acagcttatc gccttgctcc tccaggccgt ttatctcaac gatctgggtg tggatggtct
    10561 tggatctgcg gaaattggga aactcctcca tcatggtctt gagcgcggcg cagcagtctg
    10621 cgatgacctg tgcaaaagcc tgcgcctcgg gccgcagcgt gcggatattg aacatataca
    10681 gccgcagcag aacatcctca atgctgtctg tcacgtcgtc gatggtgccg gaaagctcca
    10741 tgatatcttc ccggtcaatg gggggcagga actcgcgcac aagctgttcc atcgtgccgt
    10801 gctttgcgat atcggccgag tgctcgattt tgtgcatagc ctcgatatgc ccgggcagcg
    10861 cctccggctc atagtggtca aacaccgtgc aaagcatgtc cgccgcagcg caggcggaat
    10921 ttacgccgcc cacaaaggcc tcaaaataat ccgtttcctt ttttcgtgcc attttcacgt
    10981 tctccttcct attctcagaa cagccacatg aacagccgtg ccatcaaaaa gccaatggcc
    11041 ccgcacccgg ggaacgtcag cgcccacgtc agccccattt cctttaccac gccccagttc
    11101 acgctggaca gccgccgcgc cgcgcccacg cccataatgg cggtggtctt ggtgtgcgtt
    11161 gttgaaacag ggatgccagt cagcgaggaa agcagcaggc aggccgcgcc cgccatgtcg
    11221 gcggcaaagc cctggtattt ttccagcttc accatatcca tgcccacagc cttgatgatg
    11281 cggtagccgc cgatggaagt gcccagagcc attacggcac tgcacaatac catcagccac
    11341 agcgggatct ggaaattggt gacgttgtcc tgtccgttgg ccagaaagat gcccagcatg
    11401 aaaacgccca taaacttttg gccgtcctgt gcgccgtgca tgaacgccat agccgcgccg
    11461 cccgcgatct gtgcgccgcc gaaaaagccc gtggtgcgcc gccggtccat gtttttgcac
    11521 aggaactcca ccagcctggc cgcggcccag ccgaatgcga agcccagcag agtagacaga
    11581 accaggccgt acaggacctt gatccattcg ccgccgttga tgcccgcaaa gcctccgtgc
    11641 agcgcgatgg ccgcaccgga cagcccggcg atcagggcgt ggctctcgct ggtggggatg
    11701 ccgaaccacc aggcggccgt ggcccatgcc acgatggcta cgagcgccgc gcacagcgcc
    11761 gtcagcgcgt ctgcggtgtt gccgccgaaa tccaccatgt tgtagatggt ggccgcgacg
    11821 ctggcgttca ccatggtcat caccagaacg cccaggaagt taaagacagc cgccattaaa
    11881 atggcgctgc gcgcgctcat cgcgcgggtg gaaacacagg tggcgatggc attgggcgca
    11941 tccgtccagc cgttcaccag caccacgccc agcgtcagcg ccactgtgac ggccagaacc
    12001 gggttcgcgg caagctgcgc cagaaattca ctcagggaga tcgtcaagca aagccctcgc
    12061 tttcttttta caaaaaactt actatacctt tacaagtata atggcagttc ctgacaaaag
    12121 ccagagggga aggacatgat tttacatttc ctttacataa cttgccgaaa aaacagtctt
    12181 ttccggggtc cggccataga aaaggccgga cggcacacag aaaaagccgc gcccggagag
    12241 agcgtggcac gtattgcaga aaaaacagaa aggaaaaacg ctcggagaaa agccattgtg
    12301 cctttgcgaa gtaaagtgca agccacgcgg agggtgccgc gaaacgggat ggcggcgcgc
    12361 ccgcgggcat gtgcagatgc tgtgtagggg ttctggtgca gacaaccgcg ccgaaagcac
    12421 ggcctctggt gcaaacacgc ccttttttca ttcagaacaa ctccgcgtca ccctgcaggg
    12481 ctgacgccgg ggcgtataga gagccggctc tcgaagcgca gcgtgcgcgg ctcagtacgg
    12541 cattaggcgt tacagcggca ggtcccgccg gctgaacacg gccgtgccca gcgcgtaaaa
    12601 accaagcccc agcgccagca gcacggccgc gcctgcgtac gcggcgccgt cgccgcggaa
    12661 aaggccggtg gggtcaaaca gggtgaacac ggtggcgtat ttcaattttt ccagccggcc
    12721 gcccatgttg gccagcatgc ggatgaggaa aaacagcacg ggaacacccg cgcccagcgc
    12781 cacggaaagg cggctctcgt tgaacaggca ggaggcgaaa aagcagaacc cgcccagcgc
    12841 cagatgcagg cacagcagcc cggcgttcac cacgagatag gcggggacat ccagctcgcc
    12901 gggaaacatc gcggcggcgc agcccacggc cagcgccgta cagtaggcgg taagcagcgt
    12961 accgcccgca atgagcacca gcgcctgggt gcgcgccacc cgcgcccggg tgttgggcga
    13021 ggccagcaga taggccatac tgcccgtgtc cacccagcgc gcgaccagcc ggttcgccgc
    13081 cagaatggaa aataccagag gcagaagcag cagcagaaag ccgtacagat agttgatgag
    13141 gaacccgccc agcgagctgc tgcccgcctg catgcccacc atattcatga gctgaggcat
    13201 ggccgtcacg atctcgtcca gtgtggcgtt gagcttgggg tcgaacatgg ctgtgatcat
    13261 ggacacatac agcgaaagca cggccgcaaa aaccacccat gttttccaca gcgatcttgc
    13321 gctgcgccgg aaaagcacac cgttcatcag ctctgcgcct cctttccgta atagtgcagg
    13381 aacacgtcct ccagactttg acgcggcgcg tcgatggacg tcaccggaaa ccgggccatc
    13441 gcggcggtca gagcggacat gccgtcccgc acgcgcaccg tcaccgtgca gccctgcgca
    13501 gcctgcaccc gcaggccggg cgcggccgca aaagctgccg cggcatccgg tgtggaaagc
    13561 gtgacggaat aggtgcggct gcgacgcgcc ttcagtgtgc cgatctcctc cacggcggcc
    13621 aggcgcccct cccgcagaat ccccacgcgg gcgcaggtgc gttccacctc ctcgaacata
    13681 tgggaggaca tcagtacggt cttgccgcgc tgcttttcct ccaggatcag gtcgatgaag
    13741 cggttctgca tcagcgggtc caatccgctg gtgggttcat cgaggatgag caccgccggg
    13801 tcatgcatca gcgcgcatac aatggccacc ttctgcttca ttcccttgga catgcgccgt
    13861 atccggcccg aggcgtccaa ctcaaagcgc tcgcaaagct ctccggcgcg gcccgtgccc
    13921 cgcatgccgc ggtaggcggc cagaaagcgg atgaactcgc cgccgcgcat atcgtcgggc
    13981 agggcgggct cgcccggaat atagcccata tttttttgaa tggccgcagt ctgtgtgcgg
    14041 cagtcaaggc cgttgatcgt gcaggtgccg gaatccggcg caaggaaccc catcaactgg
    14101 cggatggtgg tggtttttcc tgcgccgttt ggaccaagaa agccgaacac ctctccctgc
    14161 tccacggcga acgaaacgtc gaacacgccg tgcccgccgc cgtaatcctt tgtgatgtgc
    14221 gaaagcgcga tcacgctcat aaatattcct ccttgtaggc gatcttgcgg aacatttctt
    14281 cccagccgct gtaatcggcc aggatatcct caagccgcag cggcacgccg gcgcgctgct
    14341 tttcgttcat gtagccctcg gtcatccagg tcagcatgcg cagaagctgg gcagggtcca
    14401 caccgggacg gaatttggaa aaatcgatgt tggaaaaata ctgcgcgaag ctgccggtga
    14461 ggtccccgtt cagcctggcc tgtaccgcgt cggaaacagc ctcgccccgg gagtagaagg
    14521 cgcggatgga aaattccaac agataagggc agcgcgtcag cagttccagc ttggcccgtg
    14581 cgccgatgtg catcagctcg aaaaaatccg tcacgccgtc cagcttctgc cgcagcacct
    14641 gcccgcgcac ctgctccagc gcatagtcgt acagatacag gtacagcgac tgcttgttgc
    14701 ggaaatagta aaacagaagc cccttagaga tgcctgcctt agccgcgata tcgtcggtgg
    14761 aggcgtgctt gtaatcgttg cgggcgaaca cctccagcgc ggcgttcgta atgcgcgcct
    14821 gcttttccgg agacagtgtg aaaaattttt cgttcaaggc agtccctccc gttccgccgc
    14881 aggtttgacc ggccggtcaa gtttcattat acggcattga ccggggcgca caagccccct
    14941 gacagattaa ttttgtatta agacgcacag cggcgcggcg ggatgcctcc ggtccgccgg
    15001 ccgcgccctc cttcctgcgg agaggaagga ccgccccgca aaaaaattta cagatcgttt
    15061 tctcagccat tttccggggc gcgcggcggc acagagcatc agagcgttca aaaaagcgct
    15121 ccggaagcaa aacgggcaga tccggccgcc gaacggggcg cacgggaacg gcgtgcggcg
    15181 tcataccata gagcaggcgg ccgggtgctt gcggcccgcg ccgcaatcaa cagaaaggag
    15241 cgattgaaga ccatgaacga aaacgacctg caaaacgcag ggcagagctg ccgctgccat
    15301 ctggccattg ccacagtgcc gatgcagcgc tttgaacaga tctatccggt gtccgaggcg
    15361 ttgtgccgcg gcacgctgtt cgcggcgctg gacctgccgt tttgtcctcc ggaggtgcgg
    15421 tatgaacact gaatgcacct gtgtatccaa ggtggagcgc gaacttgccc agctcagctt
    15481 tgcaatggac gacctgcgcc tgtttctgga cacgcacccc tgtgacaaag cggcccttgc
    15541 ggcccacgag gaagtgcgcg cccggcgcgg catcgcggta aagcagtacg aacggctcat
    15601 cgggcctgta aacttctatg acgcaggcgg cacgtgtacc tggaactgga cgtgcgcgcc
    15661 ctggccgtgg gaaacggagg acgaaaacga tgtggagcta tgagaaacgc ctgcaattcc
    15721 cggtcaggat caaaacgcct aacgcggcgg cggcaaaggt gatcatcacc cagtacggcg
    15781 gcccggacgg cgagctgggc gcgagtatgc gctatttgtc ccagcgctac accatgccct
    15841 ggcgcggcgt ggcgggcctg ctgacggata tcggaaccga ggagctggcc catctggaga
    15901 tggtgagcgc cattgtatac cagctgacaa agggcctttc cgcggacgag atcaggcggc
    15961 agggcttcga cacctatttt gtggaccata cggccggcgt gtggccccag gcggcgggcg
    16021 gtacgccgtt cacgtccatg tgcttcgcca gcaagggcga cgccatcacg gatctgcacg
    16081 aggacatggc agcagatggc gcgaccggaa aagaacaaca atgttgcctc tccggttctg
    16141 tgcgtttttc ctgtgcactt gccggagcag cgaaaatata aaaaaacagc cgcacatccg
    16201 ccagggatgc acggctgctt tgcaaaagaa aatgagccaa gagattcgtt ttactttttg
    16261 attccgtagc cggacccgat atacccgcct tgctccaggt gtgccgccac ggctcgctgt
    16321 aggctggttt gcctgggaag cagagcaagg ggctttttgc cgccgaacag ccagagataa
    16381 aaagatgcgc tgtcagtgtc ggccgcgtag atgttataat ccctgccaaa acgggaaatg
    16441 ccggagtccg gggacagcag cgcacgcagc gcgggcgaga gcagccaggt gccgcacagc
    16501 acagcgcggg gcatgccgct tttacacagt gccgggccgt gcaaagcaaa aaattcatcg
    16561 gcctgtccgt acgagccggg cagcgcgtcg tctgaaagcc gggcgtcaga ggggatatgc
    16621 accgacagca caggggtgcc cgggccaata ccggatggga ggggctcatc cgcgccggct
    16681 gctcgatatt caaattccag cgtacccagc cggaacaagc ggcacgcgag gtgccgccat
    16741 gcccaaaagg cacggtcaaa tatgaaccgg ccggaacggg ctttggcctc ccgcaaaaaa
    16801 cgggagaaac agcccatagt gtcgcgaaag atttcgtccg gaatgcacat catacggtat
    16861 ttttcccgcg tgcggcaggc agctgccaaa tacagcgcca aaatctgcat gccatccggg
    16921 tcacgcccgg aaaactgggc ttccaagtgc ttccaggcag gaaatgcagt gtccggtgcg
    16981 gtgagcgcca tacattgtgc cgcacaggca tccagcagcg ccggtacagc cgtttgaaca
    17041 agctgttcgc agacgcagga gggcagttca agttcccgcg ccagtgccaa aagctgcggc
    17101 ggggaaacaa aagagcttgc gttcatacaa acgcctccct tttggcccgg ccgggcctac
    17161 ccatcgatct tgcaggcgga gaggatctcg cctacacggt agctcccgtc caacgttccg
    17221 tccgggagaa gcgtatacgg cagcgccggt tcggccagtt cggggatggt gtcacactca
    17281 cctgccgcga atgcacgcat ccggcgctgt gcggtatcca gccgcgcggc aacgcctccc
    17341 aaacggccgt cgatcacttc aaagccgtgc ggtttgttgg tggcgttcca aagcgaaagc
    17401 caggcgaggc gcagcgcttc caccgcgccg atggtctccg taaggccgtc cgcaagttgt
    17461 tttgccagtg ctgtatcatg gctgacgaca gcctgtgagg cttgttcatg ccaaacgcat
    17521 ttttttgcaa gaacgtctgc cagcatcgca taaaactgcc acagcggggc gaacgcgccg
    17581 ccctcggccg cataggcttc ataacgcgga gccagagcgg cgtaatggcg ggacagcgca
    17641 aagccctggg tgtctttggt aaacagctgg accagcgggt cctgatacag cagcattttc
    17701 gcggcgttca ccgggcggaa ttcgcccgag cgcatgcctt ccatatgatt gaacagcgtc
    17761 aggtcccaga acgcccgggc gtccgcctgg cagcagacgc gcatacgctg cgccagccat
    17821 tcatcctcaa aggagccgtg gtaggtaaat tcggcaaaca tctgcatgcc cagcagggca
    17881 gtgtagaggt ttgcttctgc gccgttgtcg ccccaggctg tggcgaacac gagagggacc
    17941 ccggccttgc ggcaggcgga gaggccggcc agcgtggcgg tgcgcgtttt gtcgtaatcg
    18001 ggcgcagggc cgcaccaggt ccagataccg cccgcgaaca ccgtgggcgc atgcagcgca
    18061 tcgtgctttt tcagctgttc gtcgtattct gcctcgcgca tatggtagta atcccagtag
    18121 acaagctcca cgccgggaac cactgcatcc acggcctgct gagagggcat cggcccgtca
    18181 taatatccgt tggtggggga ttccaggcga aagtacatat cgctccacat catgggagaa
    18241 aggcccagtt cgtttacgat atccaatacg cgcttcaaat gacggcgcaa aatatcatag
    18301 ggcttttcat agccgtgcag acgcagatga gcgcccatgc ccacgccatg agcttcgtcc
    18361 ataccgatgt ggatacgttt ggagcggtag ggcgcgctgg cggcagtgag aatctgccga
    18421 agaaaggaat aggtctcctc gctgtccgct aaaagtactt cgtcgttgtc ctgcaggcgc
    18481 cgcatcgcgg gccagtgcag cgcacggttc agatgtccca acgtctgcac gcagggacaa
    18541 agttcaatgc ccagcatatc ggcatagtca tccagagcgt gcagctcctc gtaggtataa
    18601 cggccgcgct ggtagccaaa atagggttgg ccgggaacct cgtaggtatc ctcggtgtac
    18661 atcatgccca ggttcatgcc catcagcgcc atcttgcgca aaaagctgcg gagcgtatcg
    18721 ggccgcagta ctgcgttgcg cgaggtgtcg aacatcatgc cgaccgtctc aaagcagggc
    18781 tcttcccgga tatcgcaagc tgccagcgga cggggcagca ggctgagcgc acggtagaac
    18841 tggatgggct gcgcccactc tatggtgacg gatgctccgt cgcagcaaac ggcaagagct
    18901 gcgccccggt gcccacgcac aggcacccct tccgccgcgg gcaccatgcc gagttccggc
    18961 gcaagggcgg ccagtgcatc gctgcagcgg gcgggaagct cgccggaaag cgcaattttc
    19021 aggtccatat tcatctctcc caattttgac aattttaggc cagaggcatc tccaggatac
    19081 gctccagttc acgctggtac aaccggttcg gatagctgcg ggattcaaaa aacagttcag
    19141 ccatttccgg ggtcagcaag cgggcgagga gctcgcgcag ctccgcttct atcctgtgca
    19201 gattggcata ctgtttgggc gcgcgttccc gcaggcaatc cagttccact ttgctgttcg
    19261 ggaaaagatc cccgcggaac gcggcaaaat tcaccgaggc agcatattcc tgccggtaca
    19321 tgacgtcgat gagacgggcg gtgtctttgt tgcgcgttac aagaggcaga tactgcccca
    19381 tcaatttgta gtaatcgtac cgccgctccg cgcccgttgt caactcaatt ttatacgctc
    19441 cgtggtaaac gcccataaag cgttccagaa aagccgcgct gtttgcgcgc aggttccagc
    19501 agcggtccgc ggcgtagaaa gccgtgtagc ggctcgtctc gaacagtcca taaggattgc
    19561 cgaaggagaa ggtgctgcac cagttggtgt ttaccatgcc gtcgcagcgg ctttgagcga
    19621 tcagttcaca ccaggcgtcc acattgcgca agcgttgttc gatgcaggga taattctgca
    19681 taccgcagcg atcgctggcg cggatagcgc agcagggcag cgtttttatg ccgtgggcgt
    19741 gcagagtttc catcaggcgc ggcgcaacga tgttcacacg gtcggggctg tacagccaga
    19801 tggccaccac gatgtttttg ttgatgagag cgagttcgtc atagttgcct gcttcgccca
    19861 tatcgccgtc tttccacacg ttggccagca tgtcgtgcca cacgatgggc gttttgccca
    19921 gagagaggac cttttccgcc agacggttga tgaattcaat ggcgatgcgt ccgcggcttt
    19981 tgccgctggc cctgcactcc gggctttggc ccaggctcca cacctcgtcg cagccc
//
LOCUS       DAWBSB010000011        19763 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_736_length_19763_cov_3.667901, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000011 DAWBSB010000000
VERSION     DAWBSB010000011.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 19763)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 19763)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..19763
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_736_length_19763_cov_3.667901"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    14494..14503
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    18368..18377
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2869 a         5924 c         7683 g         3267 t
ORIGIN      
        1 gagttcggcg gcagcgatga cgccggaagc cggatggtgg aggagattca gggtgaagca
       61 tatgagccgt ggttcacgcc ggttttggaa acactgctcg gcggcgagct gcccggagag
      121 atcgagagcc tgctgttttg catccagaca ggcgtgggcg tgggcgtgct ggcctacggc
      181 ttcggctatt ttgccgcgcg taaaaaatat tccgcacagt ccgtggaatg aggctctttc
      241 gcccgccgct ttgcgcgggg gctcccggtg aacatgcttt ttcaaaaagt ccggcaggag
      301 gtgggaatat gttcaacgcc ctgcttggga gctttgtggc gtggagcctg acggccggcg
      361 cgctgcccgg cccctggccg gttgcagcgg ccctggcgct gggcggcgcg ctgctgctgc
      421 tgcacggcca cacgcacggc gcggcgctga cggtggacgt gctggcgcgg cgttcccgct
      481 ttttgacggt aaacccgggc ctgaagcttt ggtgctgcgc ggccttgctg gcgctgtgcc
      541 tgctggcgcg caccccgtgg ccgccgctgg cgttgttttt cgcagcgtcc gcgttaacgg
      601 cggcgggcgg cgtacgcccg cgcgcctatc tgtcgctgct gggcgcgcct gttacatttt
      661 tgctgctgag cggtattgcg ctgctgtggg aatacgcgtc tgcgcccggc ggcgtggcaa
      721 acgtcccgtt tctgggcggg tattttgtcc tgcgcgcccc ggcgcaaacg gcggcccggc
      781 tcgttatggc gcgggcgctg ggcgcggtgg gctgcctgta tttcctcagc ctgtccaccc
      841 ccgtgccgga attgctggat gcgctgcggc gcgcccgcgt gccggaggtc gtgggcgatc
      901 tggcggtatt gatctaccgc tacatcttta ttttgttcgc cacgttccgc agcatgcggg
      961 acgccgccgc cagccgcctg ggctatgccg ggccggtgcg cagcctgcgc acgacgggcc
     1021 gggtatacgg caggctgctg gcgcacagct tccggcgggc acaggcgtgc tttgacgcga
     1081 tggagagccg ctgctatgac ggcggcatcc gttttttgac gcgggaaaag cccgtgcgcg
     1141 cgccgcacgc gcttgtgtgc ggcgcattga cggcgggcat ggtgctggcg cttgtgtgcg
     1201 gcgcatgaca cggcggtttt gtaaaagggt tggcaggagg gaaaatggaa gagatccttc
     1261 ggtttgaaca tgtttcctat gcatacgagg aagagcggga ggcgcttttg gacgtgtgcg
     1321 tgaaggtgtg cgccggcgag cgcgtggcgg tgctgggcag caacggcgcg ggaaagtcca
     1381 cgtttttcct tctggcgaac ggcgtgctgc gccccggtgc gggacgcatc ctgtttcggg
     1441 gcgcgccggt gggcggcggc gcaaagcagc tgaacgtgct gcgccggggc gtggggcttg
     1501 tgttccagga cccggaggtg cagctgctgg gcggcagcgt ggaggaggag gtcagcttcg
     1561 gccctatgaa cctgcgcctg cccgtaggcg aggtacgcgc ccgcgtggat gcggccatcc
     1621 gcagttttgc gctggaggaa taccggggcc gtgcgccgca gtatctttcc ggcggggaga
     1681 aaaagcgggt gacgctggcc gacgccctgg ctatggagcc gcagatgctt ttgctggacg
     1741 agccagcggc cagcctggac ccggcaaata cccgcctgct ggaacaaaat cttcaaatgc
     1801 tctcggagca gggcctgggg cttatggttg caacgcacga cgtggatttc gcatggcgct
     1861 gggccacccg ggtgctggtg ttccgcgggg gacggctggc gggggacggg gaaccgcggg
     1921 agatttttga aaatgtttct ttgctgcgga catgcgggct ggagcagccg gtgctgtacc
     1981 gcgtggggcg cgtgctgggc cttgccgcgc cgccgcacac tgtggaggaa ctggaacagg
     2041 agtggaaacc atgaaacagg ctttgctggc cgtcagcttc ggcacgagcg tgccgcgcgc
     2101 gcggcaggat atcgaggcgg tggaacgcgt gcttgccgcc ggggcgccgg agcgtgcgtt
     2161 ctgccgcgcg tacaccagcc cgacgatccg ccgcatcctg gccgaacggg gagagcccgt
     2221 gtccggcctg cccgaggcgc tggaggcgct tgcggccgcg ggggtggagg atgtggcggt
     2281 gcagcccacc catctgctgt acggctttga atacgataag ctcaaagcgg acgcggcggc
     2341 gtttgcgggg cgcttcgccc ggctggccct ggggcggccc cttgccgcgg acagcgaaag
     2401 cctgcgcgcg ctggctgcgt gcatggtgcg gcgcttcggg cagccggagg gcggggcgtt
     2461 ggtgctgctg ggccatggca cggagcactt cgccaacatg gtctatcccg ccatgcagac
     2521 ggccctgcgc cttgcgggcg ggcggcgcgc ctatatgggc acggtggagg gctggcccgg
     2581 ctttgacgag gtgctggcgc agctgaagga agacggctgc gggcaggcgc tgcttgtgcc
     2641 gttcatgctg gtggcgggcg accacgccct gaacgatatg gcgggggacg gcccaaaaag
     2701 ctggaaaagc aggctcgagg ccgcgggcgt cggggtgcgc tgccggatgc ggggcctggg
     2761 cgcgctgccg gaggtgcagg cgctgtacgg tgcgcggctg cgggagatag tataaatttg
     2821 taaggcgggt gacgggatgc cgtttgagca ttatatccag tccggcggaa aacggctgcg
     2881 ctgcggctat accaccggga cctgcgcggc gcttgcggcc gcgggcgcgg cacgcctgct
     2941 gctgggcggg cgcgcgccgg agagtgtcac gctcacgacg cccggcggcc tgcctgttac
     3001 ggctgcgccc cggtattgcc gcatggcagg ggaaaccgcc tgctgcgcgg tggaaaagga
     3061 cggcggcgac gacatcgacg cgacgcacgg cgcgctgatt gaggcgcggg tggagcgcgc
     3121 ggcacagccg ggcgttgtga tagacggcgg cccgggcgtg ggccgtgtga caaagcccgg
     3181 gctggaccag cccgtgggtg cggcggccat caaccgtgtg ccgcgcagga tgatcgagca
     3241 ggcggtgcgc gccgtgtgcg atgcactgaa ctgggacggc ggcctgcggg tgaccatctg
     3301 tgtgccggag ggcgcggcgc tggcgaaaaa aactttcaac ccgcagctgg gcatcgaggg
     3361 cggcatttcc attctgggaa cctccggcat cgtggagccc atgagcatgc aggcgctcat
     3421 cgacacggtg gcgctttcgt tgcgccaggc gcgggcccag ggggccgccc gcgtgctgct
     3481 gacgcccggc aattacggtg tggattttct gcgtgagcag gggctggacg cggcgggcgc
     3541 acccgtggtg aaatgctcga atttcatcgg cgacgcactg gacgaggccg cggcgctcgg
     3601 ctttgccgac gtgctgctgg tggggcacat cggcaagctg gcaaagctgg cgggaggcgt
     3661 catgaataca cacagccggt atgcggattg ccgcacggag ctgctgtgcg cccacgcagc
     3721 ggtgtgcggc gcgtcgcaaa agacctgcgc cgcgctgctg gacgcggcca ccacggacgc
     3781 atgcatagcg ctgctggacg aagcggggct gcgcgcgcct gtgctggaca gcctgctgcg
     3841 ggccgtggag cggcatctcg tccgccgggc ggcgggcgca taccgggtgg gtgcggtgct
     3901 gttttccaat gaatacggtc tgctgggccg gacggcttcg gccgcggaga tgctggcgga
     3961 atggaagggg aaaaaacaat gaaaaaaggc gttttttaca gtgtgggcgt tggccccgga
     4021 gacccggagc tcatcacgct caaggccgtg cgaacgctgg agcgctgccc tgtggtggcc
     4081 gcgccgcaga cgaaaaatgg cgagatgctg gcgctcgcca tcgcgcggca ggcggtttcg
     4141 ctggaaggca aaaccgttgt gccgctgcat tttaccatgt cccgggacaa ggcgcagcag
     4201 cacgccgcgc accttgcggc ggcgcaggcg ctgcggccgc atctggacgc ggggcgggac
     4261 gtcgccatgc tcaacctggg cgacgtttcc atctacgcca cggccgccta cctggcggac
     4321 attttggccg cggatggcta tgaaacgcgc atggtgcccg gcgtgacgag cttctgcgcc
     4381 gtggcggccc ggctgaacac cagcctgacg ggcatcgata ccccgctgca catcgtgccg
     4441 ggcggctgcg gcgcactgga ggaatgcctg gcgcagccgg gtgccaaggt gctgatgaag
     4501 tccggcaggc agctgcccgg cgtgctggcg gcgctggagc ggcgcggcct gctggagcgc
     4561 agcgcgctgg tgagcaactg cgggctgccc ggagaacagg tttatgccga cctttccgcg
     4621 ttcccgcgcg aacaggacgc gggctatttt gcaacgatca tcgtaaagga ggactgaccc
     4681 atggtgcatt ttgtgggcgc cggccccggc gcgccggacc tcatcacgca gcggggcgcg
     4741 gcgctgctgc gcgccgcgga cgtcgtgatc tacgcgggca gccttgtcaa cccggcgctg
     4801 ctgggcctgt gccggcagga ctgctcggtg tacaacagcg cgcagatgac gctggaacag
     4861 gtgctggagg tggtgcgcgc cgcccatgcg gaaaacaggg acatcgtccg cctgcacacg
     4921 ggagacccgt gcctttacgg cgccatccgg gagcagatgg acgcattgga cgcactgggc
     4981 attccctatg acgatacccc cggcgtttcc agcttctgcg gcgcggcggc cgcggcctgt
     5041 gcggaataca ctttgccggg cgtgagccag acggtcatca tcacgcgcat ggccgggcgc
     5101 acgcccgtgc cggaacgcga gggcatcgcg gcgcttgccg cccatggcgc cacgatggtg
     5161 gtgtttcttt cggcgggcat gctggacggc ctgcaggccg ctctgctgca gggcgcgtac
     5221 acgcccgaca ccccggcggc gctggtatac aaggccacct ggcccgagga aaaggtggtg
     5281 cgctgccgtc tgggcgcgct ggcacaggcg gggcgcgaaa acaatatctc aaaaacggcg
     5341 ctggtgctgg tgggggattt cctcgcgccg gattatgaac gcagcaagct gtacgacccc
     5401 tcctttgcga cggaattccg cgaggcgaag ccgtgaccgt gcggctgctg gcgttcaccc
     5461 aaaagggcat ggagctggcc cggcggctgg cggcagagct atccggcgag gccgcgcgct
     5521 gcggcgaagg ctgcacgctg gacgcctgga cagccgacgc ctttgccaac gcggacgcgc
     5581 ttgtctatgt gggcgcggcg ggcatcgcgg tgcgcgccgt cgcgccttat gccgggaata
     5641 aggcgtcgga cccggccgtg gtggcggtgg acgaatgcgg ccgcttcgcg gtgccgctgc
     5701 tgagcgggca cctgggcggc gcgaaccgtt tggcccggcg catcggcgct gtttgcggcg
     5761 cgctgcccgt catcaccacg gccacggacg caaacggagc gttcgccgtg gacgagtggg
     5821 ccgtgcggca gggctgctgt gtgctggatg tgccgcgcat caaacgggtg tcggcaaaaa
     5881 tactggcggg cggcacggcg gtgctgcgca gcccatggcc cgttgcgggc acgcccccgg
     5941 cgggcgtggc ggcggacggc ggcgcgttcg attttgaatt gacggtgcac agcgccgacg
     6001 cgggcgcgct gcggctcgtg ccgcgcatcg cggtgctggg cgtgggctgc cgaaggggca
     6061 ttgggcagca ggcgatggag gaggccttcg cggcattgct ggcgcaaagc gggctgtgcg
     6121 ccgaggcgtt ttataaggtg tgcagcatcg acctgaagcg ggacgagccg ggcctgcgcg
     6181 cgttctgcgc ggcccacggc tttgcgttcg agacattttc cgccgcacag cttgcggcgg
     6241 tggagggccg tttttcgtcg tccggctttg tgcagagcgt gacgggcgtg gacaacgtgt
     6301 gcgagcgcag cgcggtgctg ggcagcggcg ggacattgta ttgcaaaaaa caggcgggcg
     6361 gcggcgtgac catggcggcg gccatggcgc ccttcgcccc ggattggcgg tggcaggatg
     6421 aataaggtat atgtggtggg cctggggccg ggcggagagg aatttatgac ggcgcaggcg
     6481 cgcgcggcca tggaacgcgc ggacgtgctg tgcggctaca cggtgtatgt ggatttggtg
     6541 aagcatctgt acccgggcaa ggagacatat gtcacgccca tgacgcggga gctggaacgc
     6601 tgccgctggg cgctggaaac ggcggcggcc gggcgcacgg tggccatggt gtgcagcggg
     6661 gacgcgggcg tgtacggcat ggccgggccg cttttgcagc ttgcagggga atacggcggc
     6721 gcggaggtgg aggtggtgcc gggcgtcacc tcggcgctct cgggcgcggc ggtgctgggc
     6781 gcgccgctga tgcatgattt ctgcgtgatc tccctgtcgg atctgctcac gccgtgggag
     6841 cagatcgagc gtcggctggc ccttgcggcg gcgggggatt tttccatatg cctgtacaac
     6901 ccgtcctcga aaaagcgggc ggactatctg cgccgcgcct gcgatatcct gctgcgggaa
     6961 aaaagcccgg acaccgtctg cggctgggtg cgcaacatcg gccgggaggg ccagcagcac
     7021 cgcctgctga cgctggcccg gctgcgggac gagcaggtgg atatgttcac cacggtgttc
     7081 atcggcgcgg gaacgacgca ggccgtggcg ggccgcatgg tcacgccgcg gggctatgag
     7141 gccaaacgat gaaagtgctt gttttcggcg gcacgacgga gggccgtttg ctggcacggg
     7201 agctttcggc gctgggcgcg caggtcaccg tgagcgtggc caccgcgctg ggagcggagg
     7261 agcttcgggg cgttccgccg ctgacggtgt gcgtcggccg aaaggacgcg cggcagatgc
     7321 gtgaaatgct gccgggcttt gcagtgtgcg tggacgccac gcatccttac gcggccgagg
     7381 ccagcgggaa catccgcgcg gcgtgccttg cggccggggt gccgtacagg cggctgctgc
     7441 gcggggccag cgccctgccg ccggacagcg ttgttgtgcc cgacgcgccg gccgccgcgc
     7501 ggtggctggc cggcacgcag ggcaatatcc tgctggcgac gggcgcgaag gagcttgccg
     7561 cctttgcggg gctggacgcg gagcggctgt acccgcgggt gctgcccacg cacgaggggc
     7621 ttgcggcctg cgaggcggcg ggcgtgcccc acggcaatat cattgcgctg caggggcctt
     7681 ttacgcggga gctcaacgag gcgctgatgc gccgttttga catcgggtat cttgtcacca
     7741 aggacggcgg cgcaccggga gggttcggcg aaaaggcgca ggcggcgcgg aatgtggacg
     7801 cgcggctcgt ggtgatccgc cgcccggcgg acagcggaga agattacgcg gcggttttga
     7861 atgaatgcag ggagatgatg gcatgcaggt gatcctggcg gcaatgggcg gcggcgcgcc
     7921 cggaacgatg acggcggagt gcgccggggc gctggcggcg gcggaatgcg tcgtcggcgc
     7981 gcggcggctg ctggccgcgc tgcccgcggg ctgcacggcg cgcagagtgg aagcagcgcg
     8041 cccgcaggag gtgctgcagg ccgtgctgga agcgcgggga tacgcctccc tgtgcgttgt
     8101 ggcgtacagc ggcgacacgg gcttttattc cggcgcgtgc ggcctgctgc cgctgctggc
     8161 ggaaaacggc gttgcggcgc gggtgctgcc gggcatttcc agcgtgcagc ttttggctgc
     8221 gcgtctgggc cgcccctggc aggactggac gctcgtctcc gcccacggcg cggcgtgcga
     8281 cgcggctgcg gcggtgtgcg gcggcagacc ggtgtttttc ctcacgggcg gcacgctcgg
     8341 cccggccgcg ctgtgcgcgc agctgtgcga agcgggcctg ggcgggctgg ccgtgacggt
     8401 gggggagaac ctttcctatc cggacgagcg tgtgacgcag gccaccgcgg cgcagtgcgc
     8461 gcaaggtgcg tttgcaccgc tgagcgtgct gctggccgag gccgcgcccc gcgcgccgca
     8521 gcgcgcgccg ggcattgcgg acagcgcgtt tgcacgcggg gaggtgccga tgacaaagca
     8581 ggaggtgcgc gccgcggcgc tgggcaagct ggccgtgcgc ccgggcgacg tctgctggga
     8641 cgtgggcgcg ggcacgggca gcgtgagcgt ggagctggct ttggccgcgc accgcggccg
     8701 cgtgtacgcg gtggagtgcg aagcagacgc ctgcgcgctc atccgccgga accgaacgcg
     8761 gttcggagcg tggaatatga cgccggtgca gggccgcgcc ccggaggcgc tggccgggct
     8821 gcccgcgccg gatgttgttt tcatcggcgg caccgggggc ggcatggccg gcgtggtgga
     8881 cgcggcgctg gcgcgcagcc cgtccgcgcg gatctgcatt tccgcaattg cgctggagac
     8941 gctttccgcc gccgctgcgg cgctgacggc ccacgggctg gccgcgcagg tgacgcagat
     9001 cgccgtcagc cgcacccggc ccgcgggcgg gctgcacctg cttatggcga acaatcctgt
     9061 ttttctggtc acggggaatt gcgaatgatc gaatgtatgc tggccgcgcc gtcctccggc
     9121 agcggcaaaa cggcgctggc ctgcgcattg ctggccgcgc tgcaaaaacg ggggctcgcg
     9181 ccgtgcgcgt tcaagtgcgg gcccgattat atcgacccca tgttccaccg ttcggtgctt
     9241 ggcgtggaga gccataatct ggacctgttc ctctgcgggg aggacgcggc ccgcacattg
     9301 tacgcgcggc atgcggcagg ccacggcgcg gcggtggtgg agggcgtgat gggcttttac
     9361 gacggcgtgg gcggcgtgac ggcgcgcgcc agcgcgtggc atgtggcccg cacgctggac
     9421 ctgccggtgc tgctggtgct gcgcccccgg ggcgcgagcc tgacgctggc tgcacaggtg
     9481 cggggcctgc tcgctttccg gcagcccagc catatcgcgg gcattctgct gaacgattgc
     9541 tccgccatgc tggcccaaag ccttgcgccc atgctggagc aggagacggg cgtccccgtg
     9601 ctggggtatc tgccccacat gctggaggcc gcgttcgaga gccggcatct gggcctgtac
     9661 acggcggcgg agatcgacgg ccttgcggcg cgcatcggcg cgctggccgc gcagctggag
     9721 cggagcgtgg atatgccgcg cctgctggcg ctgtgcgggc ggcgcacggc agatggaacg
     9781 ggacgggagc gcaaggcccc ggcgcgaaag caggcggcgc gcatcgccgt ggcgcgggac
     9841 gaggcgtttt gctttgccta tgccgaaacg ctggaatccc tgcgggacgc gggcgcggag
     9901 cttgtatttt tcagccccgt gcatgacgcg gcgctgcccg ggggagcggg cgggctgtat
     9961 ctgcccggag gctatccgga gctgtacgcc gggcagcttt cgcaaaacgc ggccatgcgc
    10021 cgcgcggtgc gccgcgcggt ggaggcgggc ctgcccacgg tggcggaatg cggcggcttt
    10081 ttgtatcttg gccagagcct tgagggcgaa gacggcgcgg tgcaccctat ggcgggcgca
    10141 ctgcccggcg cgggcgtgcg gcggcagaag cttgttcgct tcggctacgc gcagcttacg
    10201 gcccgggccg acagcctgct gctgcgcgcg ggcgaaacgg tccccgttca cgagttccac
    10261 tattgggaca gcacggaaaa tggcgcggcg ttcaacgcgc agaagcctgc gacgggccgc
    10321 agctggacgt gcgggttcgc ggggccggcg ctgtatgcag ctttcccgca cctgtatttt
    10381 gcgggcaggc cgcagctggc ggagcggttt gtgcgtgcag ccgccgcctt tcaaagcggc
    10441 cggtgaaggg ccctgtgctt ttgagaggcc gcgggaccgg ggcggcgccg tgcgcttggg
    10501 aaagcgcccc gggcattgaa aacaaaatga agctggaatg caaagaaaga agctggaagc
    10561 atggaactgc gtgaattgct gcaaaacatc acgccgccgg acgaggcggc gcgggaacag
    10621 gcgcaccgcc gctgggcggc gtgcgcaaag cctttgggca gcctgggtct gctggagacg
    10681 gcgctggagg acattgcggc gctgacgggc gacgcgcagg cgccgctttt gccgcgtgcg
    10741 gtgctggtgc tgtgcgcgga caacggcgtg gtcgcccagg gcgttaccca gacggacagc
    10801 agcgtgacgg ccgctgtgac gcgcaacctg gctgcggggc gcacctccgt gtgccggatg
    10861 gcgcaggccg cgcagtgcgc cgtggtgccc gtggacatgg gcgtgcggga ttttgcgccg
    10921 cagcccggcg tgctggcccg gcgcatcggc aacggcacgg gggatatcac acaggggcct
    10981 gccatgacgc gggcccaggc ggagcaggcc gttttggcgg gcgcggagct tgtgcgcacg
    11041 cagaaggaaa agggcgtgcg tctgctggcc acgggcgaga tgggcatcgg caacaccacc
    11101 acggccagcg ctgtggccag cgtgctgctg ggccgcgcgc ccgtggaaat gacgggccgc
    11161 ggcgcgggtc tttcggacga ggggctgcgg cgcaaggtgg cggccatcga gcgggccgtg
    11221 gccctcagcc gccccgccgc ggacgacccg ctggacgttc tggctaaggt gggcggcttt
    11281 gacatcgcgg ggatgtgcgg cgtgtttctg ggcggcgcgg cctgccgtgt gcctgtgctg
    11341 gcggacggct tcatcagcgc cgtggcggcg ctgtgcgcgg tgcggctgtg cccggcggcg
    11401 gccaaggccg ttttggcgag ccatgtctcg gccgagcccg cgggcatgct gctgctggac
    11461 gcgctgcgca aaaaaccgct catcacggcg ggcatgcgcc tgggcgaggg cacgggagcg
    11521 gtggcggcca tgccgctgct ggatatggcc cgcgccgtat atatggattg ctacacgttt
    11581 gaggagggcg gcatcgaggc ctatacgccg cagggcggag agggctgaac gccggagagg
    11641 aaaggggcgg atgggatgtt cacactggtc gtgggcggcg cggcgtccgg caaaagcgaa
    11701 tatgccgagc agctgatcct gcgggcgggc gctctgcctc gctactatgt ggcgacgatg
    11761 gaaccctttg gcgcagaagc ccgcgcgcgc attgcgaggc accgcgtcct gcgcgcgcaa
    11821 aagcggttcg agaccattga atgccccgtt ggcctttcgc gggtgctgct gcctgcaagg
    11881 ggcgcggcgc tgctggaatg cctgagcaat ctggccgcaa acgagctgta cagcccggcg
    11941 ggcgcaggcg gtgaacaggc ggcgctggcc gctgttgtgg acggcgtgga aagcctgcgg
    12001 cggcagtgcg aagcactggt ggtcgtcacg ggcgaggtgt tcagcggcgg cagcgcctat
    12061 gcgggagata ccggtaccta tctgcgtgtg ctggccgcgg caaaccgggc gcttgccgcc
    12121 cgggcggacg ctgtttgtga ggtggcgtgc ggcctgccgc gctattataa aggagagggg
    12181 gccgcgctgt gacgctgctt gaaacggccg ccgtggcgtt tgccatgttt tccgccgtgc
    12241 cggtaccgca gccggtgtgg aacgaaaaaa acatgcggta cgcgctgtgc gcctttccgc
    12301 tggtgggggt tgtgtgcggc cttgcctggt ggggctgggc ggctttggcg gcgcggcttt
    12361 cattcccgct tttgctgcgc gcggccgggc tgtgccttgc gccggtgctc gtgaccgggg
    12421 gcatccacct ggacggctac gccgacacct gcgatgcgct ggcaagctgc gccgcgccgg
    12481 aaaaaaagca ggagatatta aaggacccgc gctgcggcgc gtttgcggtc atacgcctgt
    12541 gcacctattt tacggcctat ctgggcctgt gcgcggcggt ggactgcacg ccccgggctg
    12601 tgctgtgcat ggggctgggc tttgtgctgg agcgggcgct ttccggctat gcgatcgcgg
    12661 cgttccctct ggcaaaaaac acgggccttg cccatacctt tgcaacggga gcggaccgca
    12721 cggctgtgcg ccgcgcgctg cttgtactgt gcgcactggc cgccgcaggc atggcggcgg
    12781 cgggggggat gacgggcgcc gccatggcgc tggctgcggt gcttgtattt ttgcgatacc
    12841 gcgcggtggc gcaaaagcag ttcggcggca tatcgggcga cctggccgga tggtttttgc
    12901 aaaaggcgga gctgtggatg ctggccgcgc tggcagcgtg cagcctgctt tcgggggtga
    12961 cgggatgatc tttatcacag gcccgctgta cagcgggaaa cgggagtatg cccggctgct
    13021 gctgggctgt acccgggagg agctggcgca gcgcgctgtt tgggacgtgc aggagctggc
    13081 cgcgggctgc ggcgaccttg ccgcgttggc gcagcagctt gcccggaacg aggtggtcat
    13141 cgccaccgag gtgggcggcg gcgtcgtgcc ggtggacaaa aacgagcgcg ccgcgcggga
    13201 ggcggcgggc cgtctcggct gcctgctggc gcagcgcgcg gacgccgtgg tgcgcgtgtt
    13261 ctgcggtatc ccggtctatc tgaaaggagc gccgaacaca tgaaagttta tcttttgcgc
    13321 catgggcaga cggaatacaa tgtggacaag cggtaccagg gcacgcggga catcccgctt
    13381 tcggaaaagg ggcgcgccgc gctgcggcgg gcggacattg aaccggacac ggtgtatgtc
    13441 tcgccgctgt gccgggccgt ggacacggcc cgggtgctgt tcccggccgc gcggctcgtg
    13501 cccgtgcacg acctgcggga gatgtgcttc ggcattttcg aggggcgcaa ttatgtggag
    13561 atggagcgcg accccgatta tctcgcctgg gtgggagacg actgtatggg ccgctgcccg
    13621 ggtggagaga gccgggctga attttccgac aggacctgcg cggcctttgc gtccctggtg
    13681 gacgaagcgc tggcgcgggg cgcggaaatg ctcgtcgtca tggcgcacgg cggcacccag
    13741 atggcggtga tggagcggta cgcctgcccg cgccgggatt attacacctg gtgcgcgggc
    13801 aacgcggccg gctttatact ggatgcggcg cgctggcaga cgcacggcct gctggacctt
    13861 gcgggcgaag cgtgttatgc ggaggaagcg tgagatggat attgtgctgg ccgccctgtg
    13921 cggctttttg atggaccttg cgctgggaga cccggcctgg atgcctcacc cggtggtggg
    13981 catggggaaa tgcatcgccg cgctggaacg cgggctgcgc cgcgcttttc ccaaaacgcc
    14041 ccggggcgag cttgctgcgg gcgcggtgct ggcggccttg ctgccgctgg gcacactggg
    14101 ccttgcggcg ggcgcgctct ggctgtgcgg ccttgcgcac ccggcgctgc gctttgcgct
    14161 ggaggccgtg tggtgctggc aggcgctggc catgcggggc ctgcgggacg agagccgcaa
    14221 tgtgtaccgc gcgctgacgg gcggcacgct ggagcaggcg cgcgccgccg tggcccgcat
    14281 cgtcgggcgg gacaccgccg ccctttccgc cggaggcgtg acgaaagcgg ccgtggaaac
    14341 ggtggccgaa aactttgccg acggcgtggc cgcgccgatg ttttacatgc tgctgggcgg
    14401 cgcgccgctg gcgctgtgct acaaggcggt gaacaccatg gacagcatgg tggggtataa
    14461 gaatgagcgg tatctctatt tcgggcgcgc ggcnnnnnnn nnncactgct gctggcgggc
    14521 gcggcggcgc tcacggggca aaacgcgcgc ggcgcgctgc gcatctggcg gcgggacagg
    14581 cgcagccatg ccagccccaa ttccgcccag acggaggcgg tgatggccgg agcgctgggc
    14641 gtgcagctgg ccggcccggc cagctatttc ggcaagctgt acgaaaagcc cacgatcggc
    14701 gacgcgctgc ggccggtgga gccgggggac atcctgcggg caaaccggat gctgtatgcc
    14761 gcagggacgc tgtgcatggc gctcctggcc tgcgcgcgcg ctgcggtgtg cctgccgctg
    14821 gggctgtgac ggaggggaga gaacgcatgg aactgacgca tggaggggac tgggccggat
    14881 atgcggccca gtacggcggt atgccgctgg atttttcggc caacgtcagc ccgctgggtt
    14941 tgccgcaggg cgtgcggcag gccgtggcgc gggcgctgga cggatcggac cgttacccgg
    15001 acccgttgtg ccgggcactg cggaaaaagc tgtccggcac attgggcgtg ccgccgcaga
    15061 gcattttctg cggcaacggc gcggcgcacc tgatcttccg gctggcgctg gcggaaaagc
    15121 cgaagcgggc gctcgtcacg gcgcccacct ttgccgaata cgagcaggcc ctggccgtcg
    15181 cgggctgtac ggcggaccgc tttttcctgc gggaggaaga aggcttcgcc gtcaccgaag
    15241 cgctgctgga acgcattgag ccggggctgg acatactgtt tttgtgtgag ccgaacaatc
    15301 ccacgggacg caccacgccc cgcgcgctgc ttttgcgtat tttagagcgg tgcgccgcct
    15361 gcggcacgcg ccttgtggtg gacgagtgct tcaacgaatt tctggacgat cctgccgcgc
    15421 acacgctgct gggagagctg gaggggcatg aaaacctgct gctcctgcgg gcgttcacga
    15481 agtggtatgc gatggcgggc gtgcggctgg gctacgcgct ttgcgcggac ggcgcgctgc
    15541 tggaacgcat gcgcgccgcc gggcagccgt gggccgtttc cgctctggcg caggcggcgg
    15601 gcgaggccgc gctggaggaa acggattaca gcgcggcgct gcgcgcgctc gtcgccgccg
    15661 agcggccgcg cctttcggcg gggctggcgg cgctggggtg ccgcgtgctg ccgggcgagg
    15721 cgaattatct gctgttttac cacagcggcg agcagctggt gcagcggctg cgggcccggg
    15781 gcgtgctgct gcgcggctgc gccaattatc cgggcctggg gccggggtgg taccgcgcgg
    15841 cggtgcgcac ccattcggaa aacagcgcct ttttgcaggc gcttggggag gtgttgtaaa
    15901 tggcgcgctg cattatggtg cagggcacga tgtcgggcgc gggcaaaagc ctgctggcca
    15961 cggcgctgtg ccgcattttc cggcaggacg gcctgcgggt ggcgcccttc aaaagccaga
    16021 acatggcgct gaacagctat atcacccgcg acgggctgga gatgggccgg gcgcaggtgg
    16081 cacaggccga ggccgcgggc cgcgagccgg acgtgcgcat gaacccggtg ctgctcaagc
    16141 ccagcagcga caccggcagc cagctgatcg taaacggcga ggtgcgcggg cagtaccgcg
    16201 cggcggaata tttcaagatg aaaaaaagcc tggtgccgga gatcctgcgc gcttatgaga
    16261 gccttgcggc ggaaaacgac gtcatcgtca tcgagggcgc gggcagcccg gcggagatca
    16321 acctgcggga cggcgacatc gtcaatatgg gcctggcaga gctggtgaac gcgccggtat
    16381 tgcttgtggg ggacatcgac aggggcggcg tgttcgcgca gctttacggc acggtggctc
    16441 tgctgcagcc ggaggagcgc gcgcgcgtcg tcggcacggt catcaacaaa ttccgcggcg
    16501 acgtggagct gcttcgcccg ggccttgcga tgctggagga aaagacgggc gtgcccgtgc
    16561 tgggcgtcgt gccttatacc cgcgcggaca tcgacgacga ggacagcctt gcgccgtgcc
    16621 ttgcgcagac gcagcagcgg cgtccgttgg acatcgcggt ggtgcgcctg ccgcgcatct
    16681 ccaattttac ggatttttcc ccgctggaga gccaccccgc gctgggcgtg cggtatgtgg
    16741 agcgggccgg gcagctgggc gagccggacc tggtggtgct gccgggcaca aaaaacacga
    16801 tgggagacct tgcctggatg cgccagaacg gcctggaggc cgcggtaaag aagctggcgg
    16861 ccgcgggcac gcccgtgctg ggcgtgtgcg gcggctacca gatgctggga acggcgctgg
    16921 acgacccgga cggcgtggag cagggaggcc gcatggacgg catgggcctt ttgccctgcg
    16981 cgacgcgctt taccgggcag aaggtgcgca cccgtgtgca ggcgtgtgcc gccgcaggcc
    17041 cgtttgcggg cgcgcagctg gacggctacg agatccatat gggccgcacc gaacgcggcg
    17101 gcacgccgcc gttctgcctg cttgcggacg gcacgcccga gggcgcggcg gcaggcaatg
    17161 tgttcggcac ctatctgcac ggcctgtttg acaccgggga gcttacggaa aagctggccg
    17221 cctggctgct ggcgtgcaag gggctttcgg ccgcggacgt gcgcgcggaa agccatgccg
    17281 cgtataagga gcgtcagtac gacctgctgg cggacgcggt gcgcgccgcc gttgatatag
    17341 ctgcggtgta ccgcgcaatg gacgcctgcg cggccaagta aacacagagg agatgcgagc
    17401 gtgaacggaa aacataccct gcccgcggat atcgagcgca ccagcatggc gatcattaca
    17461 caggagcttg cggcggcggg cattgagctg ccgccgcagc atgccgccgt ggtgaagcgg
    17521 gtgatccata caacggcgga ttttgattat gcgcaaagcc tgtgcttcac gcccgacgcc
    17581 gttgcacgcg gcgtggcggc gctgcggcag ggcgtgccca ttgtaacgga taccaatatg
    17641 gcaaaagcgg gcgtcagcaa gcccagcctg gcgaagctcg gcggcacggt ggcatgcttt
    17701 atggcggacg gggacgtggc ccgcgccgcg caggaaaacg gcaccacgcg ggcggacgcg
    17761 gccatggaaa aggccgcgcg ggaatatccc ggcgcggtgc tggccgtggg aaacgcgcct
    17821 acggcgctgc tgcgcctgga ggcgctgatc gcggacgggc tgcggccggc gctggtcgtg
    17881 gcggtgcccg tgggctttgt gaatgtggtg gagagcaagg agcgcatctt cgccgcgtgc
    17941 agggcggcgg acgttccggc catagcctcc atgggccgca agggcggcag caatgtagcc
    18001 gcagccatct gcaacgcgct gctttacacg gccgtggacg cactggaccc cgcccggcgg
    18061 ggctggcagg gctgacagca ggcccggaat gtggtatgat atgtccgccc ggaagacgct
    18121 gtatgcgctg tctgtccggc cgcgggcgcg gatattttga atggatggga tgacacggac
    18181 atgaaaaaac agattttgag ttttaccgcc cttttgctgg cgcttcttct ggcgggctgc
    18241 gcggcgtccg cgccgcagag cggcagcgcg gcttctgctt cggcttccgc gtccggcgca
    18301 cagccggaaa gcgcggcgtc cggcagcgcg gatgcgcggg ccgtcacgtt caacgacacg
    18361 ctgggccnnn nnnnnnnatc ggcagctttg ccgacgtgtg gtgcctggcg ggcgggaagg
    18421 acacgcttgt tgcggcggcg gacgacacct ggacgcagtt tgagctgggc ctgcccgaaa
    18481 cggtggtgaa cctgggcggc gtaaaggagc cgagcgccga ggcgctgctg gccgcgcagc
    18541 cggattttgt catcggcagc gcaaagacgg cggcggatgt ggacctgctg cccacgctgg
    18601 agcaggcggg catccccacg gcatactttg aggtgagcac ctttgaggac taccttgcga
    18661 tgctggaggt ctgcacgcgc gtcacggggc ggccggagct gtatgagcaa tacggcgaac
    18721 aggtgcgcgc gcaggtggat gcggccgtgg cccgcgcgga gggaaaggaa gggccgtccg
    18781 tgctgtattt gcgggccacg ggcagcagct gcaaggtgaa gaacagcgaa aacagcgtgc
    18841 tgggcgaaat gctggccgcg ctgggctgtg tgaacatcgc ggacagcgac gcgtctctgc
    18901 tggaggagct ttccatggag gccatcctcg cggcggaccc ggagaggatc tttatcgtga
    18961 tgcagggcag cgaccaggag aaggtgcggc agacgctgga gagcgccgtg ctttccaacc
    19021 cggcatggca gcagctgagc gcggtgaaaa acggcagggt ctattatatg gaccagcagc
    19081 tgtacaactt gaagccgaac gcacgctggg gcgaggcata cgaaaaactg gcggagatcc
    19141 tgtatggaga agcgtagctt tcgcagcgtg cctgcgcgcc ttgcgctgct ggcgctggcc
    19201 atgctgggcg ctgcgctgct gagcctgtgc ctgggcgcgg cggcggtgtc ccccagggag
    19261 attctgtcgg tgctttcggg cggcgggaag ggcacgacgg cggcaagcat acttttgtat
    19321 gtgcgcctgc cgcgcacatt gggatgcctg ttggccggcg cggcgctggc ggtgtccggc
    19381 gcggtcatcc agacggtgct ggcaaacccg ctggccgcgc ccaacatcat cggcgtcaat
    19441 tccggcgcgg ggctgggcgt ggcgctgtgc tgcgcagtgc tgccgacggc ggctgccgcg
    19501 gtgccctttg cggcgttcct gggggcgttc gccggggtga tgctggtgct tctgatctcg
    19561 gaaaaggcgg gcgcgtcccg catgacgctg gtgctggcgg gcgtggccat ctccggtatg
    19621 ttcggggcgg gcatcgacgc ggtgctcacg gtattcccgg actccctcat cggctattcg
    19681 gatttccgca tcggcggcct ggccaacctg tccatgggca aggtcgcgcc tgcggcgtgg
    19741 gtgatccttc tgtgcctggg ggc
//
LOCUS       DAWBSB010000012        19395 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_756_length_19395_cov_3.564478, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000012 DAWBSB010000000
VERSION     DAWBSB010000012.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 19395)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 19395)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..19395
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_756_length_19395_cov_3.564478"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    8817..8826
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    16405..16414
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3862 a         5541 c         6105 g         3867 t
ORIGIN      
        1 cacacgcgca gccgtgtaaa cggcagcttt gggagttatt ataccataaa atatcccacc
       61 gagagaagtt tgtgctatac tggagaaaaa gagcggttcg cgccgcgcca atacagggag
      121 gacacggcaa tggaatatga gatcgacgag gtccggaaaa tatccatcgg cggagtggga
      181 cagaaaattc atatccgggg cgagaaaaga acgaatccgg tgctgctgtt tttacacggg
      241 ggccccggca tcagcaaccg ggacagtgtc gtcaaccggg aaaaagacct gtgcgacgtg
      301 tttaccattg ttgcgtggga tcagcggggc acgggcggat cctactgggg tgtaaaaaag
      361 gaaacgctga atctggagca gctcatctcc gacgctgcgg agctggtgga gtatctgtgc
      421 gccgctctgg aaaaagaaaa gctgttcgtc tggggcggaa gctggggcac ggaactggga
      481 acgtatctct gcttccggta tcctgaacac atcgcgggct atgtaggcag cggccagctg
      541 gtaaacggcg tactgaatga agagctgagc tatgatttcg ccatggatga agccaaaaaa
      601 gccggggaca caaaggccgt ctccacgctg gagcgtatcg gacgcccggt ggatggatgc
      661 taccgcgagg tgtttaaagg catgatggcc cagcggcgca tcatgaaaaa atacggcgga
      721 cactccatga acaaggggac ctactggacg gataccgccc tgccgctgct gaggagccgt
      781 gagttttcgt tcacagacaa gctcggcctt gcattggggt ataaaaggtg cctgacatat
      841 atgtggccca cgacgtccaa atgtgacttc ccgcgggagt gcacgcgctt tgccatgccc
      901 tattatatct ttcagggagc tcatgacaac aacacgccca gcgcgttggt gcaggcctat
      961 tatgacgcaa ttgaagcgcc ggataaggat ctgatctggt tcgagcattc ggctcacggc
     1021 cctctgcggg aggagccgga aacgtacaaa agactgctgc gagaaaagct gctgcaatgg
     1081 atatgagctg cagtgtgcac gcggacaggc ggggccgaaa caggcggtga tgcggccacg
     1141 gcggctgttt ccgcgcattt tgccccgaat gggcaggaac gccctgccgc agacaggcaa
     1201 aaaccgcatt gcaggccgtc gaagcggcgc aatacggttt ttggggccgc tgctgcggct
     1261 tggggcggga cgtcagtctg cctggatgtc gatgacgctt accgggcagc cgtcacgggc
     1321 ctcggcggcc gcaggcagct cttcctcggg aatgtccccg gccatggcga cggcgacacc
     1381 gtcctccagc gtgaatacag cggggcaggt ggaggcacac aggccgcagc cgatgcaccc
     1441 ttcgttgaca atggctttca taatgcttcc tccttctggt agttcataaa cggtgccctg
     1501 cacggcacag aggccggaga ccgctttcgc ggcggaccgc cgccctgcag agccgcatgt
     1561 ggaaaggata ggcgctttcg tgccgtttta tgcgtggaag cactgttttc ctgccgcacg
     1621 gtggaagaaa agcggaaaac aaaacgcgcg cggcagaaaa gccgagcgcg caacgtgcag
     1681 gaaaaagatg gccttacagt gtcaacccgg ccacggtcat acagcccagc tcgccgatga
     1741 tatacgctgt aatgcgcgtg ccggcgggca tagcggcggt gtccagcagg acgttgccgg
     1801 cctgggacac gttggtgtaa tagaagcgtt tgtgcgccgt ggtggcatag gcatggccgt
     1861 cctgtacggt gtaacatacc gtgcccgtgc acaggccgtc cttttcgcag aaatccgtgg
     1921 caaggctttt gcgttcgccg cggaggaaca gcgcgaattc cccggcgatc tctgtgcggc
     1981 gcagttcgcc ggtcacacgc tcgggcgggg cgacggcgcg cgcgccgtcc ttgccgtgaa
     2041 tgaaatacgc cacgccgccc caggcgtggg cgaactggtc cagccggtag cgccgctggg
     2101 ctgcggcgtc gcccggcgcg taagggtcat tgacaagcac gtactctgtg ccgtcctcgc
     2161 cggtttcaaa gccgcgcacc accatcagat ggccgtcggt acagcccgtc gtgccctcca
     2221 gcagaggcag gccgcgttct tcagcatgct ccggcgtatc cgcgtaatgc acgctcaccc
     2281 cacacgcaaa cccgtttttt atctctttct tcagtccggc gatatccgca aaggcgacat
     2341 agcactcata tccgtagcag cccgcaatgg cggtggtaaa actccagttg ccgttgcccg
     2401 cgtatgtata atcgtagttg gcgtgggcca cctcttcggg cagcaggtcc tcgccccaac
     2461 ggttcatcag catggtgacc gtggtggggc tgcagatgac agagccgatg cgcgggtccc
     2521 ggatgagctg agaataagcg ggaacaggga cgcaccggtg ctgaagcgct tcgcccgttt
     2581 cccgctgcca gctcgtggga cgtacggagg cggcaagcag atatacggag ggggaacggc
     2641 gcacgtcgtc tgtataaagg ctggcccgga gctggaacag agaagcaccg ccgggcgcac
     2701 gtacattgac catgtcggta tccagataag ctgcggcgtc ttccgggtgc tgcacaggca
     2761 ggctggcgcg gcgcaggaac ggactccatt tgccgtaggg catccagggc gtccacgccc
     2821 cggccgcaaa tacgcgcacg gcgagctcca ccgcggtgcc ggcgggcgtg gccgcattcc
     2881 acgaagcgac cagcgcattg aacgcggggg ccgaaatttc cggtgtggtg tagcagccag
     2941 cctgtacgta acgcccggca tactgctcca ggcgcagcgc tccgtctgcg taggcggtat
     3001 tttcaaacgc gccggacgca aaggcttttt cgtctttcaa aacgaacgta tggttcgtca
     3061 tatacaagct ccctcacaca aaaccgccgg ggacacgtta tatgaagcgg ttccggcggc
     3121 gttttcacaa ttttatcaca aagtatctat aataccaaat attatcgcac aagcggtcaa
     3181 aagtcaagtt acaaaacgct ttccttttcg ccgtgtttcg tgtataatga tataaaatat
     3241 caataagaac cggataccgc aacatgcggc acccggttga aggagtgaca aaaatgaatg
     3301 cactgacgct tgccgcgctg ggtacgggct ttaccttttt gatgacggct gccggggcgg
     3361 cggtcgtgtt tctgttcaag aatgagatca agccctcgtt ccagcgtgtg tttctggggt
     3421 ttgccgccgg cgtgatgatc gcggccagtg tgtggagcct gctgatcccc tccattgaga
     3481 tggccgggga acagggcgta ccgggatggc tgcctgcggc cggaggcttc gcactgggcg
     3541 cattgttcct gatggtgctg gatgccttaa tgccccatct gcaccccggc tccagcgagg
     3601 cggagggcgt acataccagc ttcaaacgca ccacgctgct ggtgctggct gtaacactgc
     3661 acaacattcc cgagggcatg gccgtgggcc tggcctgtgc gctggcggca ggcggcggct
     3721 catccagtgt gacgctggcg ggcgcgatgg cgctgtgttt gggcatgggg ctgcaaaatt
     3781 tccctgaggg tgcggccatt tcgctgccgc tgcggcgcga ggggctttcc cgtccaaaga
     3841 gctttgtcta tggtgcgctc tcaggtatcg tggagcccat cggcggcatc ctggccgtgc
     3901 ttatcgcggg cagcgttacg ccgctgatgc cctggtttct cagctttgct gcgggcgcga
     3961 tgatctatgt ggtggtagag gagctgattc ccgaggcgca tttgggcgag cattcccacc
     4021 cgggtacact gggtgtgctg gcgggctttc tggtgatgat ggtgctggat gtggcactgg
     4081 ggtgaccgat ggaacggagg tatatattcc gccgggcacg gcccgaggac gcgggggaca
     4141 ttgtccggct ttacaattcc aatccctgtt ttttggagcg tcatttggga tgccgggcag
     4201 tggacggcac gttcgttctc caagaatggc ggcagatgaa aaaggaaggg ttttccacta
     4261 cggtcgtcac ggatgcgcag accggagcgg ccgttggatt catagattgc cgggattcgg
     4321 agcctgttta tctttcgctg ttcctgttgg atgcggcgct tcaggggaaa ggcttgggaa
     4381 cggaactgta ccgggagttt gaggaaatgc agcgccgcag ggggcgcaaa ggcatccgaa
     4441 tcgacgtggt ggacgattat gaagggaacc ttgttccgtt ttggaaacgg cggggctttg
     4501 aagaaggaga agaaatcacg ctggaatggg gcggcaaggc ttcccgtgcc cgtgtgatgt
     4561 accgtgattt gatatgagtg gaccgcgcag agtaccgcgc gatcttccgg caaaacgaac
     4621 gccccgcccg aaagtcttcg gcggggcgtt tgttttattt ctgagacact tattttgcgt
     4681 aatctactgc ccgggattca cggatgacgt ttaccttgat ttggccgggg tagtccagcg
     4741 tttcctcgat tttctgggaa atgctgtgag ccagaaggat cagagcgtcg tcgttgatgg
     4801 cgtccggctt taccatgatg cgtacctcgc ggcccgcctg aatggcgaag ctgcgctcta
     4861 cgccctcgaa cccgctggcg atctcctcca ggctttccag gcgcttgata tagctttcca
     4921 gattctcacg gcgcgcaccg ggccgtgcgg cagagatggc gtcggccgcc atgacgatga
     4981 acgccagcgg cgttttggcc tccacgtcgc cgtggtgggc ctctatggca tggatgatgg
     5041 ccgggttttc cttatacttt ttggcgatgt ctacgccgat gctgatatgg ctgccctcga
     5101 tctcgtggtc cagcgccttg ccgatgtcat gcagcaggcc ggcacggcgg gcctgcgtga
     5161 cgttcacgcc gagctctgcg gccaggatac cggagagata ggccacctcc agcgagtggt
     5221 tcagcacgtt ctggccgtag ctggtgcggt agcgcaggcg gcccagcagc tttaccatat
     5281 ccgggtggat gccgtggacg cctacgtcca taacagcctt ttcgccctcg cgcttcatgg
     5341 cgagctccag ctcgcggcgg catttttcaa cggtctcctc aatgcgggcc gggtggatac
     5401 ggccgtcgct gatgagcttt tccagcgtca tgcgggcaac ctcgcggcgc accgggtcaa
     5461 agctggaaag cgtgatggcc tcgggcgtat cgtcaatgat aaggtcaacc cccgtggctg
     5521 tctccagcgc gcggatgttg cggccctccc ggccgatgat gcggcctttc atttcgtcac
     5581 tgggcagcgg caccacggat accgtggctt cgctggaatg gtcggcggca cagcgcgcca
     5641 cggcctggct gatgagctcg cgggccattg tgtcgcactc gtccttggtc tgcgtctcga
     5701 acgcggcgat gtgcattgcc ttttcgtggg tgagctgctc gtccagacgg ttgagcagca
     5761 gctgctttgc gtcctccgcc gtcaggccgg cgatggtttc catcttgtcc agttcgcggg
     5821 ccttgatctg ttcgatttcg tccaagcggg cctccacaag ctcggagcgc ttggcaaggt
     5881 cagtttcttt ctgctccaga acggccgttt tgcggtccat ggcctcttcc ttttggtcga
     5941 ggcggcgctc ctggcggctc agctccgaac ggcgttcctt gatttctttg tcggcctcag
     6001 ccttcaattg aaaagcttcg tcctttgctt cgatcagcgc ttccttgcgc ttttgctgtg
     6061 cggctttcat agcgtcgttg atcaggcgct tggcctcttc ctccgcgctg ccgatctttg
     6121 cctcggcagt tttgcggcgg taagaaaccc cggcgaaaaa tgcaatggcg gcagccacta
     6181 cgcctgcgac aacggcgatc acagcggcga tgaccggtga taccattttc agggttcaac
     6241 tcctattcgc ttttattatg aatgaaagta atctccgggg cggctgccgc gtcacatttt
     6301 gatttcaaaa aaggttaaaa aggcaaacgg caggtccgtc cgcccctaaa aattacatta
     6361 aatcatcatt catattgtaa agcgcggttt cacacttgtc aagaaattgt tacatttcca
     6421 tgaataaatc cttacgctta tacgaaaggt tgtgcaagtt tttcgcccgt gtgaaagcgg
     6481 cggaatgcgc tttgtgcttg acttttggac gcgcgcgttg taagataacg gtgtacacca
     6541 tcccgccaaa gcgatgaagc ggataatgca tgcgccggcg cgcaaaagag agggccgccg
     6601 cggcccgccg ttccccaaac ggcatgccgc gcgctgcaag cggctttgca ccgccgccgt
     6661 atgttaccac cgcggagcgc gggggagaac gggcgcattg ccctgcaact cccgccgggc
     6721 acggcccgtt acagccggca aagagtgaca cgcgccgggc gcgcccgccg tgtgtaattc
     6781 gggtggaacc atgggcattc tgcgctcatc ccgtatcagt tttacgggat gggctttttt
     6841 gttttccggc atatgccgga agaaggagga ttttgaaatg atcaacgtga cattgaaggg
     6901 cggcgccgtg aaggaatttg agccgggcgt ttcgccagcg gacgttgcaa agagcatcgg
     6961 catgggcctg tacaaaagcg cctgcgccgc cagggtggac ggcgaggccg tggacctgcg
     7021 cactcctttg aaaaaggact gcgagctgga gatcctgacg ttcgattccc cggaaggcaa
     7081 gcacgccttc tggcacacgg gcgcgcacat tatggcgcag gccatccagc acctgttccc
     7141 ggatgcccag ttcggcatcg gcccggcgct ggacgacggc tggtattacg acatcggcgg
     7201 cacggagccc tttacgccgg accagttcga ggccatcgag gctgagatga agaagatcgt
     7261 caaggcggac cttcctgtgg aaaaacggtt catcaccgtg gaggagggcc gcgagatctt
     7321 caaggggcag acctacaagc tggagctgct ggaggaatac gccgaaaagg gctggcagct
     7381 ttcggtttac cggcaggggg attttacgga tctgtgcgcc ggcccgcacc tgatgagcac
     7441 gggcgcggtg aaggctgtaa agctgttgag cacagccagc gcctactggc gcggcgacgc
     7501 tgcgcgggac agcctgtgcc gtgtgtacgg tacggcatac cccaaggccg ccgaactgga
     7561 ggcctatctg aacatgctgg aagaggccaa aaagcgcgac caccgcaagc tgggcaagga
     7621 gctggggctg tttgcactgc tggaggaagg cccgggcttc ccgttcttcc tgcccaaggg
     7681 catgctgctg aaaaacaccc tcatcgacta ctggcgcgaa atccataagc gcgcgggcta
     7741 tcaggagatc agcacgccca tcatcctcag cagggcgctg tgggagcgca gcggccactg
     7801 ggaccattac aaggataata tgtacaccac gctcatcgac ggcgaggact tttccattaa
     7861 gcccatgaac tgcccgggcg gcatgctggt atacaaaacg cagccccaca gctaccggga
     7921 cctgcccctg cgtatgggtg agctgggcct tgtgcaccgg cacgagctgt ccggtgcgct
     7981 gcatggcctg atgcgcgtgc gctgctttac acaggacgac gcgcatatct tcatgacgcc
     8041 ggagcaggtg aaggacgaga taaagggtgt ggtaaagctc atcgacgagg tgtattccct
     8101 gttcggcttt gagtaccaca tcgagctttc taccatgccc gaggatcaca tcggtgagct
     8161 ggaggactgg gagacggcca cggatgccct gcgcgaggcc atcgaggagc tgggccgcac
     8221 ttacgagatc aacgagggtg acggcgcgtt ctacggcccg aagctggatt tccacctggt
     8281 ggacgccatc ggccgcacct ggcagtgcgg caccatccag ctggatatgc agatgcccga
     8341 gcggtttgag ctggaataca ccggcgcaga cggacagaag caccgcccgg tgatgatcca
     8401 ccgcgtggtg ttcggcagca tcgagcgctt catcggcatt ctcatcgagc actttgcagg
     8461 cgcattcccc gtgtggctgg cgcctgtgca ggcgaaggtc atccccatca gcgaggcgca
     8521 ccatgcctat gccaaacagg tggagcagga gctgcgggac gcagggctgc gtgtggaggg
     8581 cgactaccgc gccgagaaga tgggatacaa gatccgcgaa gcccagctgg agaagatccc
     8641 ctacatgctc gttgtgggcg cgcaggagat ggaaaacggt acggtgagcg tgcgtgcgcg
     8701 caaggaggaa aagggcggca caaaaaccgt ggaggaattc aaggcgcagg tgttggcaga
     8761 ggttgccagc cgggagcgct gaacgcaaaa ggcagacaaa aaaggccgcg cgtcccnnnn
     8821 nnnnnntttt ttgtcgtgtt tttgcggcgg cccgccgcag aacggtcaaa attccagctg
     8881 cataaggcgg gcgatagcca ggatatagat cttcagtgcc ttgaacagca gcgcctcgct
     8941 cacgccctcg tccgggccgt gcatgggccc gacccattcg ggcgcgggtt cgccggtctc
     9001 ttccataccg aagctgacgg cgcatttgaa gtggcgcgca taggtgccgc cgcccatagt
     9061 gaaaggctgg gcgtttttgc ccgttacatc gttgtacgtc cggatgagcg tctggatgac
     9121 cggggtattg gggtcgatat agaacggaac acgggcgctg tccggcagga agctgccgcc
     9181 cgcgtcctgc gccagcttcg acagcgcggc gcacagcgtc tcggcattgg tgctggtggg
     9241 gaaacggatg tcgatgcttt ggcgcagcgc gccgttttcc agcttgatga cgccgcccac
     9301 acaggtgagc gggtcgaaca cgtcgtcctg cgcggcgatg cccacgctgc tgccgtctgt
     9361 ggaggcgtgc agccggcgca gcatttccag cgcgcgggct tcctcctcgc tacacaggcg
     9421 gttgtccagc aggtagtcca ccacaagagc aatggcgttc accgtaccgg cgggcatggc
     9481 cgcatggcca cccttgccga aagcggaaat acgagctacg ccgtccgcct cggctgtaac
     9541 tttcacattt tcggtgtcct tcagcgcgga ggcgtccgcg cgtaccagcg cataagcacg
     9601 gtccggcacc acattgtggg ccacgccgcc ctgaaagtcg atgaggctgc cgtgcagcgg
     9661 tgcgctcacc agccagccgc cgtagccgcc cttttccccg tagcacaccg ggaactcggc
     9721 gtccggcgtg aagcagaatg cgggctgggg atagtgttcc aggtagtaat ccacgtcttt
     9781 catgccggtc tcctcgttgg cgcccagcag gatgcgcagc gtatagggca gcgtggcgcc
     9841 ctgctgcttg aagaatttgg cagcatacag cgtcagtaca gcggggcctt tgtcgtcggc
     9901 cacgccgcgt cccacgacat agccgtcgcg gcgcaccata tcaaacgggg agcctgtcca
     9961 gccgttgccc tcgggcacca cgtccagatg cgtaatggtg gcgatctgcg tggggcttgc
    10021 gccgggcagg tcggcgtagc cgatgtaccc ttcgcagttg tgtgcgtcca gacccatgcc
    10081 ggcggcgatg tccagcgcag cctccagggc tttcctgggg ccggggccga aaggtgcgcc
    10141 cggctggggt tggccttcca cgctgttgat gtccaccagc gtcttcaggt cccgcaggac
    10201 attttccctg ttttccgcca caaaagcatc gatctgttcc agaagaagtt tttcgtccat
    10261 aaacaatctc cttgcatagt tgataccttt tattatagta tattaaaagg acaacacaag
    10321 acataaaaag gcgtaaaaag gagaaaccat gaaaaaaaac atattgttcg cacttgcggt
    10381 cattttgctg gcgggcgtga ttgccgtgac aagagttatg acgcgtacag aggggacgac
    10441 ggcccgcgtg gagatcacgg acgcggagac gatcaccctg ccgctggaca aggacggcac
    10501 ctatgaaatc agcgaaggca agctccccgt gacgctggag gtgtctgagg gccgcatccg
    10561 ctttatcaac agccggtgcc cggaccatat ctgcgagggc tacggctggc tttcaaaaga
    10621 gcacgaccag gccgtctgta tgcccgcggg cgttgtggta tcggtggaaa aaggcgcctg
    10681 acggccatcc tgcgcgccgc aggggaaatg tacagttttt gtaaagtggg aaaaacgcat
    10741 tgcgcacggg ccggctacac agtataattg tattgttact gtaaaaaaac tgtcaaaatt
    10801 tcgtgaagac cacgggcgtg tatggaaggc ccgttgggag agaccggagg ccatgtatga
    10861 ccattttcaa cgttatcagc ctggcgggcg gcctggcgct gtttttgtac ggcatgtcca
    10921 tcatgggcac ggggctggag aaatacgccg gaggcaggat ggagagcatc ctgcagaagc
    10981 tcacttcgtc cacctggcgc gccgtggctc tcggggccgt catcaccgcg ctcatccagt
    11041 ccagctcggg taccacggtc attgtggtgg gccttgtcaa ctcgggcatc atgcagcttt
    11101 cccaggccat cggcatcatc atgggcgcca acatcggcac cacggtgacg ggccagatca
    11161 tccggctttc cgatatttcg ggcggcggca cgctgatgga attccttaag cccgccacgt
    11221 tcgcgcccat tctggcgttt gtgggcgcgg tgctgttcgt gttcatcaag tcgccgcgca
    11281 agcgcgacat cggccagatt ttcatgggct tcggcttgct gttcatcggc atgggcatga
    11341 tgacagattc tgtgagcaca ctgcgggaat ccgagctgtt cgtccgcctg ttcaccacgc
    11401 tgcagaatcc cattctaggc gttctggcgg gtacgctcgt cacggtggcg gtgcagagct
    11461 cctcggcgtc cgtgggcatt ttgcaggcgc tttccagcac cggcctggtc acctggggca
    11521 gcgccatccc cattattttg gggcagaaca tcggcaccac gtccacatcg ctcatcgcgt
    11581 ccatcggcac atccaaggcg gccaagcgca gcgcgatggt acacgtttat ttcaacatta
    11641 tcggtacggt catatttatg gcggccatct acggcgtgaa agccgcggtt gggttctcgt
    11701 ggtggaacga cacgatgaat atgggcgata ttgcaaactt ccacacattg ttcaacgtgg
    11761 tggtaacgct gctgttcctg ccgttcacga agctgctggc caaactggcg gaaatgacca
    11821 tcccggacaa gcccggagaa aagcaggaca tcgagatccc cgtgctggac gagcgtctgt
    11881 tcaattctcc cgcggttgcc atccagcagg ccaaaagcgc cgtggagacc atggcccgca
    11941 acgcccgcgc caattacggc gaggccgtgc ccgtgctgta cagccataac accgaggtgc
    12001 tcaatctcat tcagcagcgc gaaagcgtca tcgataagct ggaggtcaat atcagcaact
    12061 atctggtaaa ggtgactgat cgtgaactga acgagagcga aagccatgcg gtaaacgagc
    12121 tgatcaattt cattgtggaa tacgaacgca tcggcgacta cgccatcaat gtggtggagc
    12181 gcagcggcga gatgttcgac aaggagatca ccttctccga aagcgcccgg cgggagcttt
    12241 cgctggtgga caacgccatc aaagagatca tggatatcac gctggcggcc ttccaggcgg
    12301 acgatacggt actggccgag caggtggagc cgctggagga gaccatcgac ttgatgatcg
    12361 acacgcttcg ggaacggcat atcctgcgcc tgaaaaacgg tgtgtgcgcc atcgagagcg
    12421 gcatcatttt cctggagatc ctgacgaacc tggagcgcat ctccgaccat tgctccaaca
    12481 ttgccgcgcg catcgtgggc aaggaatccg aggacgacca cttcgacgcc cacgagttcc
    12541 gccgcaacat gcacgacggg tatgtgccaa atttcaacga gatgctggcg cagtataagc
    12601 tgaagtatta tacaccgctg ctggaaagcc aggagacggc ctgagccgct catatgaaaa
    12661 caaaaagacc gccccgcgcc ggacgctgga acgcatccgt gcggggcggt tttctgcgga
    12721 aaagagacgg cgcaccgcga acacggcgct gtacgctgcg agacgtttcc taaaacgcgt
    12781 cctcgtcgtc ggaatagatt tcgatatact cgggggtacc ggctttgtcg aaccaagaca
    12841 gcgccgccag cgccgctgcg atggcggcag ccacggcgaa taccttcagg ataaatttga
    12901 acatagcaat acgcctcctg caattagtat gccggcattt gccggcagat gcggaataag
    12961 gggatgcggc ggtgaaaaaa gggaacactg cgaaaggaca cgcgccggtc aataaggcac
    13021 cacgacgaag gtgccgaacc cggctgcggt gggaacgccc gcacctgcgg gcacaggaaa
    13081 cgccgcgccg aacgccggtt cggaaacgtc atacaaaaag gcgttcccgg gcaggatgcc
    13141 gcccaccctg ggcacacggc cgaacgccgt gcggaacgcc tgagcgggga gcacaccttc
    13201 ggcgctccag taaaagccct gctcgtcact gccggccaga tgccgcatgg gcggcatttc
    13261 cagccggcgc agcggcacgt ccgccggttc cccggtacgg tacagccaca gcgcatcgcc
    13321 ctgctgcttt gtacaggacg cgaacagata agtgccggca gcgtcgtccg gcgtgacggc
    13381 aaagccgaag cgggccgtgt cgggcggcgt ttcatcaaat accgtgacgc tgaaagggat
    13441 ttcgccatcc cgcacatagc agcgcagata cgcatatacc tgcccgcggg caggctcgcc
    13501 gttgaaatgt gtgattttca atgcgggcag cgtttccatg ggcagcgcgt cccggctgtt
    13561 tgtgataaga aaggacaacg tgtttcctcc ccgcgggcgg caggcccgcg ttgctgaatg
    13621 tatgttttga acgatataca gccatgcggc cgtacgcggc aaaccgcata agacgcggct
    13681 gttttgttat tacagtatac cgctgttagt atagcacaat cgtgcataaa aacgcaaccg
    13741 ttccgcgccg gggcgcgcgg cggcacaggg aaaaagctgc cgccgcgcag gccgcgccgg
    13801 atattgtgaa aaaattatcc gttgttgact acgcccggcc cagtgtttac aatagaagat
    13861 ggattatttt gcgtactggc gttgtatctg cgccccgggt ggatacggcg gcaaccgtaa
    13921 aaaatacaaa aggagaaacg agggatcata ttttcatgtc atccaaattc aaacgcagcg
    13981 ccctcggcgc gcgcgtgccg catgaaaaga aaacatccgg tatggccact gtcgccatgc
    14041 ccctgcccga aaagatcgtg ctgcccatgc agcagcatat cggcgcgccg tgcaccccca
    14101 cggtgaaaaa gggcgatgcg gtgctggtgg gtaccgttgt gggcgcggcg ggcggtttcg
    14161 tcggcgcagc catccacagc ggcgtgtccg gcaccgtgga ggccgtggag accgtgcata
    14221 tgcccaacgg ccgttcggta ccggctgtcg tcatcagggc cgacggcgag cagacgccgg
    14281 acccggcctg tgttccgccc gaggtgacgg accacgcttc gctcattgcg gcggtgcagg
    14341 cgtgcggcct ggtgggcctg ggcggcgcgg gtttccccac ggcggtcaag ctctcgccca
    14401 aggatctttc ggccatcgac accctcgtca tcaacggcgc ggaatgcgag ccgtacatta
    14461 cctcggacaa ccgggaattt ctggaatgct ccgagagcgt gatgcgcggt attgcggccg
    14521 tcaagcagca tctgggcatc aaaaaggtcg tcatcggcat tgagcgcaac aagcctgagg
    14581 ccatcgacct tatgttcagc cttgtgaaga acgacccgga ttattccgtt atgcccctgc
    14641 agagccgtta tccccaaggg gcggaaaagg tgctcattga aaagaccacg gggcgcgaag
    14701 taccccgggg cggtctgccc gcggacgcag gcgttatcgt gctgaacgtc accacggtgt
    14761 ccacactggg caaatacctg gccacgggca tgcctctcac gaccaagcgc ctgaccgtgg
    14821 acggcgacgc catcggcgaa gcgaagaacg tagaggtcgt catcggcacg cccatcggcg
    14881 acgtgctgga cttctgcggc gtaaaggacg atgtttccaa gatcctgatg ggcggcccga
    14941 tgatggggac tgccgtagcg gacccggctt tccccgtgct gaagcagaac aatgcgctgg
    15001 tggctttcgg cccggcggcg gccgctctgc ccgctcccgg tccgtgtatc cgctgcggca
    15061 actgcatcaa tgcctgcccc atgggccttt cgcctgtgga gatcgcgggc gcgtacacca
    15121 agaacgacgg cgagatgctg gacaagctga tggtggacct gtgcatcggc tgcggaacat
    15181 gcagctatgt gtgcccggcc aagcggcccg tcacccagac gatgaatctg gccaaggccg
    15241 tgtggcagaa aaataagaac ggagggaaga aataacgatg gaaagcaaaa aactgatcgt
    15301 taccgcgtcc ccccatatca ccaaccatac cagcacgcgg gggcttatgc tcaacgtgtg
    15361 catcgcgctg ctgcccgcac tggtggccag cacgcttatt ttcggcgcgc gggcactgct
    15421 ggtcacgggc gttactgtgg cggcctgtgt gggcttcgaa gcgctgtact gcgtcgtgat
    15481 gaaaaagcca gttccggtgg gggacttctc cgccgttgtc acaggcatga tcctggcttt
    15541 caacctgccc gcgaccattc cgctgtggat cgccatcatc ggcgctttcg tcgccatcgt
    15601 ggtggcaaag cagctgttcg gcggtctggg cttcaatttt gcaaatcccg ccctgttggg
    15661 acgcatcgtg ctgttcatcg gctttgcggg ccgcatgacg gcttatgcct ttccccagac
    15721 ggacattgat gcgctggcca gcgccacgcc tctcgtggcg ggcaacagca tggcaggcaa
    15781 ggacatgctg ttgcagctgc tgctgggcgc ccacggcggc gtattgggcg agacctgcgc
    15841 cgtggcgctt attctgggcg gcatttacct gatcgccacc aaaacgatca gcgcggccat
    15901 tccgctgtcc tacatcggta ccgtggcagt gctcagcttg gtgctggggc aggatgtggt
    15961 gctgcagctc ttttccggcg ggcttttgct gggcgcgttc ttcatggcga cggattacgt
    16021 caccagcccg ttcaccttca cgggccgtat cgtttacggc gtatgcctgg gcgtcatcac
    16081 ctgcatcatc cggttctacg gcaacatggc cgaaggtgtc agctttgcgt tgctgctgat
    16141 gaacctgctg gtgccttata tcaatgacct cacgcgccag aaaccgctgg gaggtgccaa
    16201 agccaaatga aaaacacctg gaatgatttt gtaaaaccca tcgtggtgct gggcgtcatc
    16261 tgcctggtca caagccttct gctggctgtg acaaacaatg tgacggcccc catcatcgcg
    16321 gaaaacgccg tcaaaacggc aaacgccacg cggcaggctc tgctgccgtc ggcagacacc
    16381 tttacggaag tggcgccgga aaccnnnnnn nnnngggcta cggcggcgag gtgcccgtca
    16441 tggtggcgtt caacgccgaa ggcacgatcg aagccgttca attcctggac aacgacgaaa
    16501 cgccgggcct tggccagaaa gtcaagagcg aagcgttcca gagccagttt tccggtatgc
    16561 ccgccgaaga attcggcctc tccgacatcg atgccatcac gggcgcgacg atttcctccg
    16621 gcgcggctgc ctcggccatc aacgcggcta tcgcggcgta taatcaggaa gccggcgtgg
    16681 caggagagga cctttcggcc ctttcgcccg aagagctgcg cgcggcgctg ctgcccgacg
    16741 caggcgccat cacgcctgtg gacgtctccg ccgacggtgt gacggaggct tacaaaggcg
    16801 aagcttacgg cgttgtgatc tatgcgcagg cggagggctt ttacaaaaag ccggtgaccg
    16861 cggcggttgg attcgacgac gacggcgtga tcacgggcgt atacatcgac gcctccaatg
    16921 aaacggcgga tgtgggcgga caggtgggcc gtccgcggtt tgccgggcaa ttcgtgggca
    16981 agacgagcct ggacggcgtg gacgccgtgg cgggcgcgac gatctcctcg aacgcggcga
    17041 tggatgcggt gcagaaggcc attgacgcgt tcagcacagt gaaaggggcg tgagcggaat
    17101 gaaggaaaaa ctgaaggtat tctccaacgg ccttttgaag gagaacccgt cgctgcgcct
    17161 ggtgctgggc acctgcccca cgctggccgt caccacgctg gccgtcaacg gtctgggcat
    17221 gggccttgcg gccacgtttg tgctggtgtg ctccaacatc gccatttcgg cgctgcgcaa
    17281 gatcatcccc gataaggttc gtctgccggc ctacatcacg gtcatcgcca cgtttgtgac
    17341 ggtactgcag atgctggtga aggcgtttgt gcctgcgctg gattcggcgc tgggcatctt
    17401 cctgccgctc atcgtggtga actgcatcat cctgggccgt gccgagatgt tcgcttccaa
    17461 gaacagcatc ggcctttcgg cgctggacgg cctgggcatg ggtctgggct ttacgggaac
    17521 tctggtggtg atgggctccg tccgcgaggt gctgggcgcc ggtacgctgt tcggcgtaca
    17581 ggtcatgccc gcggcagtgg accccatgac tgtgttcatt acccctcccg gcggcttctt
    17641 cgtgttcggc tgcctgatgg cgctggtgat ctggatcgag atcaagacaa acaaccgcaa
    17701 ggtgcgcagc atcggctgcg agggctgccc ctcggccagc gtatgcggcg gcaaatgcgc
    17761 ggataaagcg gaaggaggcg aggcgtaatg gctgttaagc tggcaatgat cttcttcagc
    17821 atgatcctgg tgaacaacta cgtgctggtg aagttcctgg gcatctgccc gttcctgggc
    17881 gtttccaaaa agctggattc cgccgtgggc atgtctgccg ccgtcatctt cgtcatggcg
    17941 ctggccacca gtgttacctg gcccatctac acctatttgc tggagccaaa cggcatggct
    18001 tatctgcaga ccattgtgtt tatcctcatc atcgcggtgc tggtgcagct ggttgagatc
    18061 atcctcaaaa agttcatgcc cccgctgtac aaggcgctgg gtgtgtatct gccgctcatc
    18121 accaccaact gcgcggtgct gggcgtgaca atttccgtgc tggacgaggg ctacaacttt
    18181 gccgagagcc tggtgtgcgc tgtgggcgcg ggcgtgggct tcatggtggc catggtgctg
    18241 ttctccggcg tacgcaaacg gctggaggat gcccagcctc ccaagtgctt tgagggcctg
    18301 cccatcacgc ttgtggcggc aagcgtcacc agcctgtcct tcatgggctt cggcggcgtc
    18361 atcgaaaata tcttcgcggg ccattaagga aggagagaga gcatgagtat cgtaacagcg
    18421 gtggcggttg tcaccgtcat cggccttctg ggcgccgcga tcctggttgt ggcggcacat
    18481 tttatgcacg tagaggaaga cccccgcgtg ggagaggtgc tgggcgtgct gcccggcgct
    18541 aactgcggcg cctgcggcta cgccggctgt gccgactatg caaaagccat tgtcgagggc
    18601 gcgccggtga acaaatgtgt gcccggcggt gcaaaatgtg cggccgcggc tgcagccatc
    18661 atgggcgtgg aagcgggcga agttgtgaag cgcaaggcta tcgtagcctg ccagggcagc
    18721 tacgaccaca cgcaggataa gtatgagtac gaaggcattg aaagctgtgc cgcctgcgcc
    18781 gcgctgtatt ccggccgcgc cgcctgtgaa tacggctgtt tgggctacgg ggactgtgtc
    18841 aaggcctgca agttcgacgc catcacggtg tcaaacgggc ttgcccgtgt gaatccggaa
    18901 aaatgcacag gctgcggcgc ctgcgagcag gcgtgcccca aaaaggtgat ctggattcgg
    18961 ccggagagcg aaaagcccgt tgtcatgtgc gccaatcacg accgcggcgc cctgacgcgc
    19021 aaggcctgca cggcaggctg catcggctgc atgaagtgcg aaaagagctg cccggagggc
    19081 gccatcaagg tgaccaataa cgtggcccgt gtagactatg aaaagtgtac gggctgccgc
    19141 acctgtatga atgtgtgccc ggtgcatgcc atcaccatcc caaagatcgt ttgaccggta
    19201 ggttcccggc tttgctgacc cgccctgctg cttttgcagc ggggcgggtt tctgcataca
    19261 tggcgtgatg caaaataccg tgcccgtttc cccaaaagga tgcgttttgc aaaaatttgt
    19321 tttataatac aattataaga tatttatttt agtatataaa ccatgggaaa tgctctcacg
    19381 gcagtgtata atacg
//
LOCUS       DAWBSB010000013        18974 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_774_length_18974_cov_3.521063, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000013 DAWBSB010000000
VERSION     DAWBSB010000013.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 18974)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 18974)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18974
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_774_length_18974_cov_3.521063"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5195..5204
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3550 a         6322 c         5478 g         3614 t
ORIGIN      
        1 ccagatggga aagcttgccg tattccttga atttcttagc gcggaaatac acgtcgccgt
       61 ttttggccac atagccgtag cctttttcaa tgatgtccgc cacgatggac aatatctcgt
      121 cgatgtgctc cgtggcgcgg gggcgcaccg tgggcggcag tgcgttcaga ccgtcgcaat
      181 cctttaaaaa ctccgcctca taacggcccg cgtactcctg catggaaacg cccgcctcat
      241 tggccttggc gatgatctta tcgtcgatgt ccgtgatgtt ggaaacaaac agcaccctgt
      301 ttccctgata ctccagatag cggcgcagcg tatcgaacac caccaccggg cgcgcattgc
      361 cgatatgcac atagttgtac accgtggggc cgcatacata aatgcggtat tcccccggca
      421 ccagcggcgt gagctcttcc tttttgcgcg tcatcgtgtt gaatattttc attttatgtt
      481 cctcctccgt tcggggtggg tttcattcag agcgccgcag acgcagcatg cggtccgcgc
      541 ccagcagcac gcccgcgcgg gcggcggcaa aattcaggcc gccctgcata tacacggtat
      601 acggcgcgcg cagcgggccg tcggcggaaa gctcgatgct gctgcccatg gtaaatgcgc
      661 ccgccgccat gatgacggga tcctcgtaac cgggcatgtc ccccggttcg ggcgctgcaa
      721 agctgtccac aggactgccc gcctgcacgc cgccgcacag tgccaccatg ccctccgggc
      781 tgcccgtctc cacactggtg ataatgtcgt tccgctgcgc gccgtaagcc ggggtggccg
      841 cataaccaag aagctcgaac agacaggctg catacacgct ggttttcagc gcctcgcaca
      901 ccacgccggg cgcatagtac aggcccagat acatgccgcg cagcatgtcc agcgtgcagc
      961 cgatctcccc gcccacgccg ggtgcggaaa ggcggtgggc gcaaagctcc accagatcgg
     1021 cccggcccgc gatgtagccg cccgtgggcg cgatgccgcc gcccggattt ttgatgaggc
     1081 ttcccgccat caggtccgcc ccgcgttggg tgggctcctg tttttgggtg aactctccgt
     1141 agcagttgtc caccatgacg atgatatccg gctgcaccgc cttggctgcg cggctgacgg
     1201 cttcgatctg ctccagcgaa agcgcgggcc gcgcggcgta accgcggctg cgctgaatgt
     1261 aacacatctt tgccccgcgg cacttccggg caatgcctgc aaggtccggc gtgccgtccg
     1321 ccagcagatc cacctcatcg tactgcacgc cgaattccat cagatttccg taatgggtgc
     1381 cgtcgatacc gatcacgccg gccagcgtgt cgtagggacg gcccgtgacg gcgaccatgc
     1441 ggtctcccgg gcgcagcaca ccgaacagcg ccacactgag cgtatgcgtt ccggacataa
     1501 agttgtggcg gaacagcgcc gcctcactgc ccgtcagcac agcgaacact tcctccagct
     1561 ttccgcgccc cgcgtcgtcc atgccgtagc ctgtggtacc cacaagatgg ctgccgccca
     1621 cgcggcagtc cgtgaaagcc ttcagcattt tcagctggtt gtattccttg atttcttcca
     1681 gccgggcaaa ctgctcccgg cacagcgcca gcgccctttc gtctgcgtcc agcagcgggc
     1741 gcgacagatc ataaaactgc tcgatcattc cctgtcctct ttcatcgtag tgtcggcggc
     1801 acgccgccct gtttttcctc ccggctgcgc aggcccttta tttttcctcc ggcagcccgt
     1861 ccagcgcata gcgctgcacg gtatcctccg ccgcaaagcc aagacggtgg taaaaattca
     1921 gccgttcttt tttgcaggag agtgccacac gccgcccccg ggctgccaga tccgctgcaa
     1981 gcaggcccgt cagccagccg ccgatgccct gcccgcgcag cgcttccaag gtttcaacgg
     2041 cggccagata tgcggtatcc ggcgaaaggg cgtaagcgcc cgccgtagaa acgagcgcgc
     2101 cctcccgccg cacggcccac acctcggccg cgccccgggc cagctttgtg cagagctcgg
     2161 aataaaaatt gtcccacgcg ccggggtcgt cctcgccgcg catcataaaa tccgtcacct
     2221 cggcgggcgc gggcgcgcgg tccagcgtca ggcggacagc gtccgggcac ggcggagcgg
     2281 cggctttcgc cagcaccaga ttgtgcagcg cgtacagagg actgtacccc gccggggccg
     2341 cgccgccggc attcacatac cgtacgccct gcatacgcag gaacgcgccc gtctcctccg
     2401 cgtcgtatgt accgcacagc acggcgctgc ggcccttgag ctgtacggcg gcggaaccat
     2461 ccgccacata aaagcgcatc agcgcgccgc tgcggccgaa tacggccaga tgcacgggca
     2521 ggatatcccc gaaatagggc ctgccccgca ccgcggcaag aaactgcgga cgattgctct
     2581 cattcagttc cgcgatcaca gcgcgttcac cagctctttg taatggcgcc ctcgcgcctc
     2641 aaaattggga tagctgtcaa agctggcaca ggcgggcgaa agagacacga tatcgcccgg
     2701 ctgcgccgcc gctctggcca tctctacggc ctgaggcatg tccgccgccc gcaaaatcac
     2761 aagcccgctt tcggcaaagc cggggtccgc cttcaccgca tcttcgattt tctgcgcggt
     2821 ggctcccgta aggatgagtg ttttcacatg agcgatcagc tccggcgcca gcggcgcaaa
     2881 gctgatgcct ttgtccgagc cgcctgcaat aatgatgagc ttctggtcaa acgcccgcag
     2941 ccctgcgatg acgcgcgtgg ggctggtggc gatggaatcg ttgaaccact gcacgccgtt
     3001 cagcgtgcgc accggctcga tgcggtgctc cacgcctgca aagcttttgg cgacggcggc
     3061 catggtggag acctccgcca taccccacac ggctgtaaac gccgccagca ggttttccag
     3121 gttatgctcg ccccgcaggg ccacgtcctc gcggcgcacg atgggcgtta cgcgtccgtt
     3181 ttccgccatg cacagcatgc cgtccgcccg caggaaacca ccgttttcca ccacgctctt
     3241 acgcgtgaac cagcggcagc tcccctttac atccgcggcc atggcgcggg tgacctcgtt
     3301 ttcatagccc agcacggctt ttccgcccgg cttctggtac aggaggatat tgcgcttggc
     3361 gtcgatgtat tcctgcatat ccttgtggtg gtccagatgg ttgggcgtga cgttcgtcac
     3421 caccgccacc tccggggact ggcgcatgct gatgagctgg aagctggaaa gctccaccac
     3481 ggctacatcc gtggccgcga tggcgtccac cacaggcagc agcgcgcggc ccaggttgcc
     3541 gcccagatgc acggtgtacc ccgccgcctc caatattttt gcaatgagcg tcgtggtggt
     3601 ggtcttgccg tcggagcccg tcacggccac gatcttgcag ggacaaagcc ggaagaacag
     3661 ctccatctcg ctggtcacct cggcgcccgc ctcccgggcg gccaccagct ccggcgtata
     3721 gtattcaaag cccggcgtgc gcatgatcat ctcctgtccc gccagcccgt cgaggtaatg
     3781 ctctcccagc gaaaaccgga tgtccagctt gcgcagcgcc tgggcatacg ggccaaaggc
     3841 gtcgatatcc ggctttttgt cgcacagcgt gatctgcgcg ccgcaccgtg cgaacagctc
     3901 aatgcactgc tggttcgtga cgcccgtgcc gatgaacgca acgcgcctgc cgcgcagcga
     3961 acggtaaaaa tcctcgattt gtttctccat tgtcccacct cattcgcata agtatagata
     4021 gtatagcata tttatggtaa gtttaaaagg ttcgcgggga ttttggagga ttttccgccg
     4081 tgtttccgca ggctctgcgc acacgctgct ttttccgctc tttttcacac cgccgggcaa
     4141 cattttgtct tgtgcgtccc ccgccccgca tggtaaaata tagggcgtac agaaaaaaca
     4201 ggacacgccc gcgccgggaa tcgttttccc tgccgggcct cataggggag gaaccgtttt
     4261 tggccaatga attgttcggg ctgttcggca gccatctgtt cgacgaccgc gtgatgcgtg
     4321 agcgcctgcc gctgagcgga tatgaatcgc tgcgtgagat tcgcgacgag ggcaagccct
     4381 ggaacccgga ggttgcggat attgtggcag acgccatgca ggcctgggcc atggagcagg
     4441 gtgcgacgca ttacatccac tggttcagcc ccatgacggg cacgggcagc ggcaagcacg
     4501 acagctttat cgatggtgta aagcagggcg agcctatctt gaatttttcc ggcaaaatgc
     4561 ttgccaaagg cgagagcgac gcttccagtt ttccttccgg cgggctgcgc gccacattcg
     4621 aggcgcgcgg atacaccgcg tgggacccca cctcccccgc ttttgtgctg ggcaccacgc
     4681 tgtacattcc cacggcgttc tgcgcctata ccggcgaagc gctggaccag aaaacccccg
     4741 tgctgcgcag catgcaggcg ctcaacaaac aggctctgcg tgtgctgcgc gcgcttggcg
     4801 ataccgaaac ggtgtctgta cagcccactg tgggcgcgga gcaggaatat ttccttgtgg
     4861 acaaggacct gttcgaccgc cggcttgacc tgaaggtctg cggccacacg cttctgggcg
     4921 cgaagccgcc caagggacag gagcttgagg accactacta cggcagcatc aacgaacgcg
     4981 tgcgcgaatt catgcgcgaa ctggacgaag agctgtggcg ccttggcgta cccgctaaga
     5041 ccgagcacaa cgaggtcgct cccggccagt acgagctggc cagcgtattc gccacagcca
     5101 atatcgcctg cgaccacaac cagatcatga tggagctgat gcgcaaggta gctttgcgcc
     5161 acaattttgc atgcctgctg cacgaaaagc ctttnnnnnn nnnnttctga atcccaccga
     5221 caaacccgag gaaaaccctg tttttcttgt aacgctgtgc gcggtgctga cggctgtcga
     5281 caaatatgcg gacctgctgc gcctttcggc cagctgcccg ggcaacgagg aacgccttgg
     5341 cggcaacgaa gcgccgcctg ctgtggtgag tatcttcctg ggcaacgcac tgacgcatat
     5401 gctgcaggag cttgccgagg gccacgcact gcaaaaggaa gagcgcggcg cgcttctgac
     5461 gggcgtaaaa agcctgccgc ttttaaagct ggatgattcc gaccgcaatc gcacctcgcc
     5521 tttcgctttc actggcaata aatttgaatt ccgcatggtg ggtgcgagcc aaagcatcgg
     5581 ccttgccaat gcggtgacaa acgctattgt ggccgacgtg ctgcacgatt ttgccgacaa
     5641 attggaaggt gcggacgatg tttccgccgt ggtgcgggaa atcgtggctg agacctggca
     5701 cgcccacggc cgcatcattt tcaacggcaa caattacagc gccgaatggg ccgccgaggc
     5761 cgcacgccgc ggccttccca atattcaaag catggtggac gcagccgacg gcttcatcgc
     5821 ggagaagaat gtagccatgt tcgagcgcac caaggtgttc accgctgcgg agtgccgctc
     5881 ccgatatgag atcacgctgg aaacgtacgc caagcatatc cacatcgacg cctccacgat
     5941 gttggagatg gcaggccgca gccttcttcc cgccagctat gaatatctgt cccgcgtgag
     6001 ccgcgcggaa tattatgcgg agcggaacgg ctgcgcctgt gccagcgcca aggcgcaggt
     6061 ggaaaaactg tgcgtcacca tcgacaagac cgcgggcgcc atggaggcgc tgcgccgcgc
     6121 tctgcatgcg ctggacactg cacatttggg tgaaaaggcc cgggcggacg cctgccgcgc
     6181 gctgcgcgag cacgagatgg ccgccctgcg cgaggccgcc gacgagctgg aggagcttgt
     6241 gccccagacg tactggccca tgccggatta ctgcgagctg ctgttcgacc tgtaaggccc
     6301 gctgctcacg gcttctgtgt gcaaaaatca tactgcaaac gcaaaacggc cccgttccgg
     6361 gcggctgcaa gccgcgggac agggccgttt ccttattccg ccgggggccc gggcagcaca
     6421 cgccgcagcg caaagccgca gcccccgccc ggcattttcc gaaagcccag cttttcgtaa
     6481 aagccctcct tgccttcggc cgcaataagc tgtacgctcg tgcgtccgcc cggaggcagc
     6541 cggctttggg cataggccag aagcttttct gccacagcct ttccaatgcc gcgcccctgg
     6601 tattccggcg cgaccaccac gtccgcgatg acgtcgtaca gcccgtcccc accaaacgcc
     6661 ccatggcaac ggcgcggcca tcctccacgg cggtcacgtc atacagggcg gcggcaagcg
     6721 cgcgcctcgt ctgttcttcg gaatgggttt cccaccccgc aagcgtgcgc aaggcacggt
     6781 aatcctcgta gcacaattcg ttttcccggt attccggcat caattttctc cctttcgttc
     6841 tcccgcgtcc cgcccgtgcg cacggagtgg gacgcagcat atccttgccg tttccgcagg
     6901 tgctatccca ccgggacaaa gggcggcggc tcctctctcg ggccgttcac agcgtatacg
     6961 gcccgcagga tctcatcccg gcgcaccgcg gcctcctgct ccggcagctc cagccaaacg
     7021 gcgtgcaggt cgtatggccc gtaacggaaa tgtcccatca ccgcgccccg tatcacgccg
     7081 tccaccaaaa gatactgcgt tacctcgctg cccttttccg ggcttttgta gcgctccttt
     7141 cgtgcgcttt ccccgcagcg gaccagcggg tcgttccggt gcagcgccag cacgccgcgc
     7201 agcggctcaa aggcctgcgc ctgcagaagc ggcacatcct gcgcgggcag atagccctcc
     7261 cgccagcgaa ccaggatgcc ctgccgcgcc agctcttcca tcgccgcggc aacgtccctt
     7321 gcgggaaggc cgtaaaaatc ctttgcatct gccgccgtca ggtacacctg ccggtgcgca
     7381 agccgcggca gcacccgcag cagggcttcg gcgcgggaga tgcgcgccgg gtccacatcc
     7441 gggaacatct cgtcaaacag gtaccagccc cggtcccatt cgccgtcgaa ctggtcctca
     7501 aacacaagaa aggcttcctg cagccggtgc agcgcgggcg tgatctgctt caccagcagg
     7561 cccgtcatcc gtttcatcgc ttcgatgttc agcgggcctt cccgcccgat cagctcccgc
     7621 agcagctccg aatgctccgt gggcttatag ggcctgcggt acaggccggc aaacagctcc
     7681 atctgtgccg cctccacaaa cccgatgttc ccgttctgga accgtccttt caccagaatg
     7741 cgctgccgct ggcgccggcc gttgtattcc acgtcgtcaa aatccgcgcg ctccaccagg
     7801 cacggcggct gcccgaaccc gtgccagtgt acgttcagcc ccggcgaaag gtcccggtac
     7861 agcgcatcgt actctgccgc gcccgcgcgc cgcgtcaggc aatggcgccg catgcgcagc
     7921 gccgtaacgt tttccattgt cagcatcctg ccgcctcccc tgttccgccg cccgtgcggc
     7981 gcttctgttc catgctatca cagggaaccc gacagctgta tgtcatgtcg gtcccacagc
     8041 acgccgcaca tgcaaagcgc cccgcgcaga gccgcagcgc ctccgcgcgg ggctccgccc
     8101 gcttttactt attctggatg gcgtccaccg cgttgatgac agccgcgcgc ataccgccgc
     8161 gctccagctc cgccacgccc acaatggtcg tgccgccggg cgagcacacc gcgtccttca
     8221 tcgcgcccgg gtgggcgccc gtttccacct gcatgcggcc cgtgcccgcc accatctggc
     8281 cggcaaggcg gtaggccatg gcccgcggca ggccgtgctt tacgcccgcg tcgcccagcg
     8341 cctcgatgaa catgctcaca aacgccgggc cgcagccgct cagtgtgcct gcaatgccca
     8401 gcaggcggct ctccacgggc tgcaccagcc ccaaatggcc gaacagctcc tgcaccagca
     8461 cgtattcctc tggcgtaagg gaatgctttt cctccagcag gaacacgccc gcgcacacgc
     8521 gcaccggcgt gtttggcatg gtgctcaaat ggcgcgttcc gggcagcagc gcctcgtacc
     8581 gctcgaaggg ccagcccgcc gccacggaaa cgactgcctt atccgcaagc tgcccgcgca
     8641 cgggcgaaag cacctcctcg atcaggtgcg gctttacggc cacgatgacc acatctgcgg
     8701 cctccgccac ctcccgggcg tcccggcagg gacgcatgcc ccggggctgc gttgcggcgc
     8761 acagcttgtc ccaatccttc gcacaggcgc agatctgctc cggctccagt gcttttgaac
     8821 gcagcagccc ctcggcaatg gcctgcgcca tattgccgaa cccgataaat cccagcttca
     8881 tatcctttct ttccatcctt ggtcttcccc actttccaat tttttctgaa cagcccggcc
     8941 ctcagctggg caggccgtcg aacgggtcct ccggcttagg cagcggcccg ctgcccgcaa
     9001 agtggcggcg cgccatccgc agcgcgacgc cgcccagcat caccagctgc accagctggg
     9061 ccagaacggc tgcataggta gtgccttgca gctcccaccg ggcgatgcac ggcccgctgg
     9121 ccagcgcgga gacggcaatg gcgcacacgt ttgccacaat cagccccttc atacagcgca
     9181 tcaccgtcag caccatgctg taaaaaagca ccagcgccgt caaaatggcg gacacgacca
     9241 taggccccag caggtactgg taatcggcca ggccgggcga gacgaacgtc gccagccccc
     9301 agcgtcccag aaaatgcgcc acagccaggc ccaggggcag cagcgccagc acaatgccct
     9361 gcacagcgaa caccgccttg aaaaagccct ttttgtcccg cctggcatag ctgtccgcaa
     9421 acacggtgat gaacggattg aacaggtaag tcgcccccac ctgcagcagc acgacgggct
     9481 gcgtgacggg gccgtaaatg cccagcgccg tctctcccat ccggctgctg agcgccaatt
     9541 tggggatgga ggccgtggtg gtgttcaaaa agctgtacac cgccagcggc aggcactccc
     9601 acagcagcgc ggccacgcgg gcgttctgcg gctgctccgg cgcataaaaa gcccgcgcct
     9661 gcggcagaga ataaacgaaa aacaccacga ggctcgcgcc cagcatcaac gcaagggtca
     9721 aaacgatatc ctgggtgagc cacagcgtca gcgtaaagga tgccagcgtc accgcgccgc
     9781 gcaccgcata ggtcttgccc acgatgtcca gccgcccgct gcgctggtcg atggcgttgt
     9841 agatatcggt aagggattcc accatgcggt agcccaggaa cagcaatacg cagacgctct
     9901 gctgggcgct gtatgggttt tcgcccgtca gggcgctcac cgctacgaag ccgatgcaca
     9961 gcgccacagc cagcgcacag gtccatacac ggctgcggat gtaggtgctt gcgccgtact
    10021 taccccggat gtccgacacc tgaaagctgt acatgccgta ggatgcgatg gacagaaaaa
    10081 tgcccgtggc cgtaagcgcc gtattgtaca cgccggaaac ctgctccccg gccaggcgta
    10141 ccaccaggat accgcacagg aactggcaca gcatataggt gagctgtccg aatgtattcc
    10201 acaaaaaatt ccgtctcaag gtctgggcgt cctgcaaagc gcggcgcctc cttttcactt
    10261 attttcccgc acctttccgc gcggcggcgg gcggcagccg ggctaacaaa aatagtatac
    10321 cacccgaacg gcaaaaaaga aagacgggcc aaagcccgcc tctcctgcac agctatttcg
    10381 ccgtgacctg cagacgtgtc agcgcgcgct tcaggcgcgc ctgtgccaat tccagctcgc
    10441 gctggtcctt ggccttttca atcagctgct ccgcgcgctc cttcgctcgc tgggcgcggt
    10501 ccgcgtcgat atcctctgcc cactcaaagg tggtggccac aaggcgcaca ttgtctttcg
    10561 tcactgccag catccccccc gcgcaggccg cgcggcggcg cttgccgtca atggtcaccc
    10621 gtgcctcacc cgtgcccagc gccgttacat agggcgaatg ccgggccagg atgcacacgt
    10681 cgccgtcaat ggcccgcacg atgagctttt ccgcctcgcc gtcgaagaaa ccgccgtccg
    10741 gcgtcacgat ttgaagatga aaggttgaca tggcgtctcc cccttacagc gtttttgcct
    10801 tctccagcac gtcgtcgatc gtgccggcga agaggaacgc ggattccggc agatcgtcgt
    10861 gcttgccctc gatgatctcc ttaaagccgc ggatggtctc cttgagcggc acatatttgc
    10921 cctgcatgcc cgtgaattgc tcggccacgc tgaacggctg ggacaaaaag cgctgtattt
    10981 tgcgcgcgcg ggagactgtg agcttatcct cctcggacaa ctcatccata cccatgatgg
    11041 cgatgatgtc ctgcaattcc ttataccgct gcaggatgcg ctgcacgtcg cgcgccacct
    11101 cgtagtgttc gcggcccacc acgtccggcg tcaggatgcg ggagttggac tccagcgggt
    11161 ccacagccgg ataaatgccc aggctggaaa tgctgcgcga cagcactgtc gtcgcgtcca
    11221 gatgggcgaa ggtggtggcc ggcgcggggt ccgtcaggtc atcggccggc acatacacgg
    11281 cctgtacgga agtaatggag ccctttttcg tggaggtgat gcgttcctgc agggcgccca
    11341 tttccgtggc cagcgtgggc tggtaaccca cggcgctggg catacggccc agcagggcgg
    11401 aaacctcgga gcccgcctgg gtaaagcgga aaatattgtc gatgaacagc agcacgtcct
    11461 ggttttcccg gtcacggaaa tattcggcca tcgtcaggcc cgaaaggccc acgcgcatac
    11521 gcgctcccgg cggctcgttc atctggccgt acaccagcgc cgttttggaa agcacgccgc
    11581 tctccttcat ctcgttgtac aggtcgttgc cctcgcgggt gcgctcgccc acgcctgtga
    11641 ataccgccgt gctgcttggc gatgttgttg atcagctcca tgatgagcac ggtcttgccc
    11701 acgcccgcgc cgccgaacag gccgatcttg ccgcccttgg cgtagggggc gatgaggtcc
    11761 acgaccttga tgcctgtttc caaaatctcg gtggtggatt cctgctcgtc ataagcgggc
    11821 ggctggcggt ggatgggcca gcgctcctga gcctccggcg cgggaagatt gtccacaggg
    11881 tctcccagca gattgaagat gcggcccagg cattgatcgc ccacgggcac cgtgatgggg
    11941 ctgcccgtat ccacggcttt cgcgccgcgc acaaggccgt cggtgctgga catggcgatg
    12001 cagcgcacaa catcgtcgcc gatgtgctgc gccacctcca gtgtgatggt gtcggccccg
    12061 ttctccaccg tgatggcatt cagcaggccc ggcagatgcc cgtcggggaa tttgatgtcc
    12121 agcacaggcc cgatgatctg ggtgactgtg ccgatgttct gattgctcat tggcttgtct
    12181 cctttgaata ggatgaaaac agctttcggc tgtgtgcgcc tgcgcttcgc gcggtcccct
    12241 gcgagggcag gcttgaaata cctgcagggc gtttccggat gtgcggagca tacgccgaaa
    12301 aacgggaagc acccgttttt caggcgtggg acgcggggcg tccattgagg actgttctcg
    12361 tcgcttcgct cctcgaacgg ctaaggcacc cgcttggcgc tccggcgcat ccggcgcgct
    12421 acccgcgcgc ctctgtgcgc ctgcgcttcg cgcggccccc tgcggggggt aggcttgaat
    12481 cgccctgctg gcgtttcaag ccaagtgtct tacgcctccg cgccggccac gatctccgtg
    12541 atctcctgcg tgatggcgcc ttgccgcgca cggttgtatt tcagtgaaag atcctcgatc
    12601 atgtcgcccg cgtttttgct ggccgcatcc atcgcagtac gccgcgcccc ctgttcgctg
    12661 gccacgctct ccgccatagc cccccacaaa aggccgccca catattccgg aacgatcgcg
    12721 tcgtataccg cctcgctgga cggctcgtag atcgtcaggc tctccgccgt gttctttgct
    12781 tccctgcgct cgtagcggat gggcagcatt ttcagcaccg ccggctgctg gctgagcatg
    12841 gacacaaaat tggtgtatcc cacatacagc gcgtcaaacg cgcccttgcg gaactgcccg
    12901 cagagcatcc gtgcgatgga aaagcaatcg cccagcgaga cgtcctcggc cgtggcatag
    12961 gcgtccgtca cggtctccag cccgcggtgc gcacaatatt ccacagcctt tttgccgatg
    13021 ggcagcacgc acaccacctt gccgtccata tgctcggcca ccgttttaaa caggttggta
    13081 ttgtagccgc ctgccagccc gcggtcgccc gcgatcatga tatagcaggt ctttttcact
    13141 tcgcgctgct gcacgaacgg agaggaaaaa tccgtgttgc cgtacgcgat gtctgtaagg
    13201 cattcatgca gcgtggtgaa atagggccgc gtctcctcgg cgcgttcctt cgcgcgccgc
    13261 agcttcgagg acgccaccag ctccatggcc ttggtgatct gcatggtgct ttccacgctt
    13321 ttgatgcgca gcttgatatc cttcatggac gatgccgcca tgacgccgtg cctccttctt
    13381 ccttcttttt atgcgtggga tttgaggaac gccgccgtga actccgccag cgccttgtcc
    13441 agcgcggcct tcgtcccgtc ggaaagcacg ccgctctccc ggatgggcgc cagcacgtcc
    13501 gcgtcatgca ggctgcttag atcctcgtac agctcctgct cgtacacagc gatctgctcc
    13561 acaggcacgt ccaccagata atcgttggta acagcataca ggatgcacac ctgctgctcc
    13621 acaggcaccg gagaattgcg gtcctgcttg agcacctcca caatgcgctc gccctgcgcc
    13681 agacggcgct tggtatccgc atccaggtcc gacccgaact gggcgaagga ctgcagctca
    13741 cggtactggg aatacgccag cttcagcgtg cccgccacct ttttcattgc cttgatctgc
    13801 gcgttgccgc ccacgcggga caccgaaatg cccgggttga cggcaggcat cacgcccgcg
    13861 tggaacagct ctgtttccaa aaagatctgg ccgtccgtaa tggagatgac gttcgtgggg
    13921 atgtaggccg aaacgtcgcc ggcctgcgtc tcgatgatgg gcagcgccgt caaactgccg
    13981 ccgccgtatt cgggggccag gcgcgccgca cgctccagca gacggctgtg caggtaaaac
    14041 acgtcgcctg gatacgcctc gcggccgggc ggacgccgga tgagcagaga aagcgcgcgg
    14101 taggccaccg catgcttgga aaggtcgtcg tagatgatca ggacgtccct gcccttgtcc
    14161 ataaaatatt cgcccatagc gcagcccgcg tagggcgcga tatactgcac cggggccagc
    14221 tcgctggctg aggccgagac cacgatggtg tagtccattg cgccggccac agtgagcgtt
    14281 tccaccagct gagccaccgt cgagcgcttc tggccgatgg caacgtagat acacaccacg
    14341 tccttgccct tctggttgat aatcgtgtcc gtggcgatga cggtcttgcc cgtctggcgg
    14401 tcgccgatga tcagctcacg ctggccgcgg ccgatgggga acatgctgtc gatggccttg
    14461 atgcccgtct gcagcggcac gctgacgctt ttgcgctcaa tgatgccggg tgccttgcgc
    14521 tcgatgggcc gcgtttcggc tgcgtcaaca ggccccttgc cgtcaatggg ctggcccagc
    14581 gcgttcacca cgcggccgat gagcgcttcg cccaccggca cgctgacgac gcggcccgtg
    14641 cgccggaccg tatcgccttc cctgatgccc tcgtcggagc ccagcagcac gatggctaca
    14701 ctgttctcct ccaggttcaa ggccatgccg tattcgccgt tctcaaactc caccagctcg
    14761 ttcgccatgc acttttccag cccgtaggcg cgggcgatgc cgtcgcccac aagaatgacg
    14821 gtgcccgttt cggcgcattc gatcctgctg tcgtaatttt tgatctggct tttaatgatc
    14881 ttgctgatat cctcaggttt taactgcata gtctcaccaa ctttccaacg tacagatgtg
    14941 caggcgtcac gcctgcgtca gcgcgcgccg cagcgcggcc agccgcccgg cggccgtgcc
    15001 gtccagctcc agcccctcgt accgcagctt tacgccgccc agcacggacg ggtccacagc
    15061 ggtctccagc aggatggtcc tgcctgtctt ttgagccaga ctgtcgcaca gggcacgacg
    15121 ctgcgcctca tccagaggca cggcgctcac ggcctgcacg ggcagaatgc cgcgcgcctc
    15181 atacagcagc gccttatact cctgcaggca gcccgcagcc atgccgagtg cggatttccc
    15241 gcacagtatc ttcaaaaaat tcagcacata aacgtgcacg cccgcgccga acgcctcgtc
    15301 cagcatggcc aggcgttctt cccgcggcac cgcggggttc tgcaccagct tcaggtagcc
    15361 gggcgtctcg cggaaagcgt ccgccgcaag ggtaaggccc tccagcaccg cgtcctcgca
    15421 gtgctcctcg gcggcaagtg cgtacagcgc gccgccgtat tccttttcgg ctgccgtcat
    15481 gacgcgtcac ccacacggct gatgaagtcg tcgatcagtt ccttgtgcgt ttcgggatcg
    15541 atctccttct ccacaacgcg gcccgcaatg tccagggcaa gctcggaaat atcgtttttc
    15601 aactctccgg cagccttgcg gcgttcgctc tcgatctcgg cgtccgcctt gctcttgagc
    15661 gcggccgcct cggcgcgcgc cgcgctcaca agctcctcgc cgcgcgcatt ggcgcgtgcc
    15721 gtggcgcttt tcacgatctc cgccgcctct tcctttgcgc tggcaaggcg tgtctcgtat
    15781 tcctctttca ttgcctgtgc gtcgccctcg gccttttcag cctcggtgta ggacgcttcg
    15841 atctcctctt tacgcttggc aaggatctgc tgcacgggct tgaacagaaa atgcttcacg
    15901 cccgccgtca ggataagcag gttgcagatc tggaaaatga acgtccacgg ggcgagggtg
    15961 acgaggtcct gaaattgttt cattctgtct cctccttttg gcggcgcggc ctgaacgccc
    16021 gccgccggtg cccgcggctt acagcaggcc catgaagatg ccgggagcca cgaagatgag
    16081 cagcaggccc gttacgaagc cataaatacc ggtcgtctcc gcaatggcgc agccaagcag
    16141 catggtggat gtcacgtcgc ccttgcactc gggctggcgg ccgatggcct cgcaggcctt
    16201 gccgacggcg tagccttcgc cgatgccggg gccgatgccc gcgatgaggg cgaggccggc
    16261 gcccacagcg cagcctgcca ggatgattgc tttttccaac atagaggttt cctcctacat
    16321 aaaaatagtt tgggttattt tcagcgccct tgcacgcagc gggcggcgga aaaacaaacg
    16381 ggagcttcgc gtccgtcagg aatcgtcggc cgcgttggcg atatacagcg ttgtcagcat
    16441 gcagaaaatg aacgcctgca taaagctgga gaacaaatcg aaatacacgc tgagcaccgc
    16501 cggcacgccc acctgcagga tggggatcga gcccagcagc tcgcctgccg cacccggcag
    16561 ccacgcaaac agtgcgtggg atgccgttgc cagcgccgca tacaccagcg ccgtgatgac
    16621 gctgccggac acgatgttgc cgaaatgacg gaacgccatg gaaatgggcg tcgccagctc
    16681 ggacaaaatg ttgaacggcg taagcacgaa gatgggctgc gtgaagccct tgaggtagcc
    16741 gccgacgccg cccgtgcgga tcttggtgta ggtgatgagc acgaacacca tcaccgccca
    16801 cgccagcgtg gtggaaaggt cgctcgtggg ggggaacatc cccaccaggc tcagcaggct
    16861 ggaaaacagc gacgatgcga aaagcgccgc cacgaacggg ccgtaatatg cgaatttttc
    16921 acccatattg ccgttcacga aattctgcgc ggtctccacc aggaattcag ccaccacctg
    16981 ccgcctgctc accgcccgca ccttcaggtt ccgggtgagg aatatgctca gccctatgat
    17041 gatgagcgtg accagcaggc tgttgacaat ggtggcggtg atggggattc cgccgaagat
    17101 cgggatggta aaatagatgc gcgcgccggt gatatccaca ttcataccgg ctcatcgcct
    17161 ccttccttat ttttgggcgg tttgtacatg cccaggatct gcattgcaag aatggtaagc
    17221 cgcgggaaca gcagcggaat caccgtagcc acccacgaaa agcacgccag ctttacgccc
    17281 agcaccacca cggccagcgt ggccagcatg cgcaggctgt acgagagctg cagcacgagc
    17341 ttgccttttt tttcgttcgg gtccccggcc attttctgca ccgtgaggcc cagcagaaaa
    17401 aagttcagca ccgcaaagcc gccgcccagc agcgtgcccc acagcaccgt caggtcccat
    17461 cttcccagaa gtgcgaacac gccgttcatc accgccgaaa aggccagcgt gcccgcggcg
    17521 atgtgcagcg tctcgcgctt tgtcgcttcc tgtatcttca taaaaagtcg atcacatcca
    17581 gctccggcct ccgggcgccg cccggaacgc cttgttttac ggccgtacag ccgctacttt
    17641 tttccgtttt tgcggcggaa cattttcaca aattccgcaa aggtacacgc cgcggcgccc
    17701 aagcccagca ctatggccgc gaaatatacc cacggcccca gcgaaaagcg ccgcgcaagc
    17761 cagcccgcgc ccagcaggca cagcaccggc ggcgccgcca gcgaaaggcc cagctgcgtc
    17821 agccaggaca ggttctgcaa aacggaaaac cagccttttt tcttcacgcg gcacgccccc
    17881 tctgcggcgg gcctgcgccg cgctccagcc ttttgtcaag ctttttgcaa atatccttta
    17941 tgcaaacagc accgatattt attatatctg aacacccacg caaatacaat ccgtaatccc
    18001 ggtgttcccc gacaggcccc gtataaattt tcacaaaaac tttgtttgat ttgttcagaa
    18061 accctcgaaa aaatccatat tgcggaatgc atgacaaaaa attgtcgata agacgccctc
    18121 cgaaaggtgt ctccacgcgg gagaacatcg ggtttggacg gaccggttgg cgcgctgcgc
    18181 atccaaaccc gccgagccgt gtcccgggcg gtaaggcata ttaccgccat cgctgcacga
    18241 acggaccgtt tgctcaaagg cttcacggaa gcctttgccg ccttcggcgg ctgcaaaaag
    18301 gaaaattgcc gcaggccgcc tcaaatttct gtttttttcg cgccgcacgc cttgccttgc
    18361 acatgggtta atggtatcct atataagata ttgggatttt ccggaacagg gccagtatac
    18421 catcggggac gcggggcggg caatagacaa acccatttgt ttgtgtataa aaacagacaa
    18481 ttccgcctga atgtataagc gcatgctccg gaaaagaata cgcaggaaga gggagcataa
    18541 cagtggaagc aggatttgac aacagccttt atatccacaa gcagacggag catatccgcc
    18601 agcgcattgc gcagtggggc ggcaagctgt acctggagtt cggcggcaag ctttttgatg
    18661 attaccacgc ggcccgggtg ctgccggggt tcgaccccaa cggcaagatt cgcctgctgt
    18721 acgagatgcg cgaacaggct gagatcatct tcaccgtctc ggcgggagac attgaaaaga
    18781 caaaaatgcg cgcggatctc ggcatcagct atgatatgga ggtgctgcgt ctcatcgacg
    18841 acatcacgca catgggcatc cgcgtgaaca gcgtggtcat cacgcagtac accggccagg
    18901 cggcagccga cggcttcgcc aaaaagctga cgatgcgcgg cgtgaaaaac tacatccacc
    18961 gccccatcaa gggc
//
LOCUS       DAWBSB010000014        18127 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_813_length_18127_cov_3.894810, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000014 DAWBSB010000000
VERSION     DAWBSB010000014.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 18127)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 18127)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18127
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_813_length_18127_cov_3.894810"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3258 a         5317 c         6173 g         3379 t
ORIGIN      
        1 gccctatgtg tatagccaga tgatcgccgg gagggacgcg gccgccttcg gcgaggcgaa
       61 aaacagctgg ctgacgggta cggccgcatg gacattcgtc aacatcagcc agtatatcct
      121 ggggatccag cccacgttgg acggcctgcg gatcgccccg tgcatccccg ccgcgatgcc
      181 cggtttcacc gtcacgcgca cctacagagg cgcggtgtac gaaatcgcag tggacaacgc
      241 ggcaggggtg gaaaaagggg taaaatgcat cacggcggac ggcgcgccct gcgtggaagg
      301 cattctgccc gccgcgcccg ccggaacgac agtacatgta aaggtgacga tgggctgaac
      361 ggaggggaca tatggcgttt gcaaaaaatt ttgtatgggg cgcggcctcc agcgcttacc
      421 agaccgaggg cagcccgcgg gccgacggcg gcggggaatc catttgggac gtattctgcc
      481 gccgccccgg cgccgttgcg gacgggtttg acggttccgc cgccacggac gcgtaccgca
      541 gatatgagga ggacattgcc tgcgcggcgc agatgggcct cggtgcttac cgtttcagcg
      601 tgagctgggc gcgggtggac ccttacggcg acggccgctg gaacggggaa gggtttgcgt
      661 tttatctgcg catgctccag gcctgccgcg ctgcgggcct ggagccttat gtcacactgt
      721 accactggga actgccccag gcgctggaag atgcgggcgg ctggcgcagc cgagagaccg
      781 cccgtgcttt tgcccgctat gccgccgagg cggcgcgccg tttcagcggc cttgcagaga
      841 attacattac gctcaacgag ccggagtgta ctgtaaagct gggccatggt acgggcgtgc
      901 atgccccggg cctgcgtctg cccgaggcgc gccagcttct ggtgctgcac catcagcttt
      961 tggctcacgg ccttgccgcc gcagccattc gggagacctg tcccggcgtg cgcgtcggca
     1021 ttgccgcaac agggcggctg tgctggcccg gcacggacag ccccgccgat gtggaggctg
     1081 cctgcagcgc caccttcgcc acgccggaca gcgactgggc atttacctat cagatggtgc
     1141 tggaccctgt ctgtttcgga cattatccgg aaaactgcgg gcccggcctg cggcgtatga
     1201 cggcggcgct cccggcggaa gacctggcgg tcatccacat acggccggat tttatcggct
     1261 tgaacatcta caatggccac gcggtatgtg cagggacggg aaagcggccg gtttatgtgc
     1321 cgcgcccgcg cggctgtgct gtgacagcgc tgaaatggcc cgttacgccg ctggtgatga
     1381 actggggcgt gcgttttctc tggcagaggt acggccggcc gatttacatc accgaaaacg
     1441 ggcagagctg caacgaccgc atctttctgg acggccgtgt ccatgatccc gaccgcattg
     1501 atttcctgca ccgttatctg ctggcgctgc gtgcggcggc ggagggaggc gcggatgtgc
     1561 ggggctattt ccactgggcg ctcactgaca attttgagtg gagcgagggc tatcgcgagc
     1621 gcttcgggct tgtttatatc gactatgtta cgggtgcgcg catcccgaag gacagcgctg
     1681 cctggtatgc cgaaaccgtg cgcgccaacg gggagaacct gtaaaagcgc atggcatgca
     1741 ggctgcagaa tacccccgac agcccggaaa agaaccggac gccgggggca ttttgcggtt
     1801 tcatgcggag cctgtatgcg tgcgctgtgc ttttcgggcg cggacaggca gacgcagcgt
     1861 gaatgtggcg cctccgccgg gtgtgtctgt aagaaggata tctccgccgt gcagcgcggc
     1921 cagttcctgt gccacagaga gacccagccc atagtgctcc ttgcccgtgc ggcttttgtc
     1981 ggcgcggtaa aagcgctcga acacatgtgc cttttccgca tccggcacgc cggggccgtg
     2041 gtcctccacc gaaagcagta cccggccttt ttccgcgcgg gcgcgcagcg tgacgtcgct
     2101 gccggcgggc gcgtaggcgc aggcgttgct gatgaagacc agcagcagct ggcgcagccg
     2161 tccggcgtcg ccccacaggg gcggcagtgt ttcctcgggc aggtccagcg caaggcgcag
     2221 gccgcgcgct tttaccagag gcgcaaactg ttcatgcgcg ccgatcacca gcgcatcggc
     2281 atctacaggt tccatacgca tccgccaggt gcccgcgtcg ctgcctgcaa gcaggagaag
     2341 atcgtccacc aggcgggcca tgcggtcggc ctcgctgccg atggccgcca ggtactgcgg
     2401 cgtgtccgcc gggctggcct gcatggccga caggctcgtc ttgacgacgg ccagcgggcc
     2461 gcgcagctcg tgtccggcgg cggccacgaa ttcccgctgt ttttgcagtg cttcggcggt
     2521 gggacgcagc gcccgccccg ccaaaaacca gttgaccgcg cccagaagtg ccagcccgcc
     2581 cgacagcagc gccgcataca gcagggcctg gcgcgtcagc gtccggcgca gaggcccgtc
     2641 atcccgcagc acggcaaggc cgtaccagcc gcgctgtccg gggacggagc atacggcggc
     2701 ggtataatgt tcgcctgcgt tgccctccac tgtgacggtg acgcgggagg catacacaga
     2761 cgaacgcggc gcggcatcca tattcacacc ctctgcaagc cctgcctctg ccgcacgctc
     2821 cagcaaggtc ccgcgcgggg tggcgggcgt ccagccgccg ctgaaaaaca gcggggtacc
     2881 gttgtccgtg atgaaaatga gcagtccatc cgcggcctcc gtctgggcaa gagccgtgtc
     2941 ggagacgctg cccgtctgca gcgtgacgat cagggtgtcc acgctggcgt cgaacatgct
     3001 ctgtgccgag agctgcgtct gcttttgtgc caaccccagg gtgatggcca ggaataccgt
     3061 gagaactgtg cccgtcagca cggtgaaaac aaatgtcagg cggcggcgca gctggcgcag
     3121 cggcgtggtg tacgggccgc ggggaggctg tgcggcacga cgtttcatgc gcccgttcct
     3181 ccatccagca gccggtagcc cacgccgcgc acagtgtcca ctgtcacccg gctgctcacg
     3241 gcacgcagcc tgcggcggat aaagtgtaca tagctgtcca ggctggcttc ctctacgtcg
     3301 gcgcccggcc cccagacacg gccgaataaa aaactgcggg acaatgtttg tccggcgttt
     3361 tccatcagtg cggccagcag ctccgcctct tttttcgaga gcgtgcattg ctctttgtct
     3421 ccggtcagca ccagtgtgcc catgtccaga gtaagatccc cgcagcaggg gcggccggag
     3481 accgccacag gagcggggcg gcgggccagc gcgcgtaccc gggccagcag ctcgcgtacg
     3541 tcgaaaggct ttaccagata atcgtctgcg cccgcgtcca gaccgtccac tcggtcgccc
     3601 acaaggccca gcgccgtcgt catcagcacc ggcgtgtcca ggccctcggc ccgcgctgcg
     3661 cgcagcaccg aaagcccatc ccgtccgggc agcatcctgt ccagaatgca cagcccgaac
     3721 atccgttggc gcatgtagta cagcgcatcg tcaccgtctc ggcatacgtc cacacggtag
     3781 ccctcctgct ccaacagcgg cgccacggcc gcgcacaggg cttcgtcgtc ttccacaagc
     3841 agtatccgca tgtttttgtc cctcctgttc cgcaataaaa ccattataat ggatgcaacc
     3901 ttaaaaatcc ctgaaaaaca gaggctttca caaatccctc actgtttttc aaggactttt
     3961 caaggttgat ttttttatga tggaaacagg ctggacggga agcatcttct gtccgcatca
     4021 ttttgagcat accggaaagg tgagggaaac agtgaagata aaacaacttt tgtgcggcgt
     4081 actggccgcg gcggtgctgt tcacgggctg cggtgcgcag cccggcacga gcggcggttc
     4141 aagtgcgcag ggggacggca cgcccatgca ggccaagggt cggttcgtgg aaaacgacat
     4201 caccccgccg ggcgcggcgg gctgtacccc ggtgagcctg acggcacggc cggacggcgg
     4261 attggactac atcgcgctcc ggcctgccga gacgcccggt caatatacat atacccattt
     4321 ttattctgct gacagcggca aagaatggac ggaaaatacg atggactggc aggagagttt
     4381 cggagagaaa cgccagatcg aaaaggtcac cgttacccct gagggagaat attttgtaat
     4441 gttgacagag cagacagagg acggtatggg cgcggtggcc tacgcacgcg ttgcggcgga
     4501 cggcacggcc gttcccgtgc agccggaggg cctttccgcg cagggcgggc ttgtggcgac
     4561 ggatgcacag gtgctgccgg gcggcagact gctgctcacc agctttgggg gcgattttgc
     4621 cgcggtgcag aacggagctg caggggaaga cgcaggcgcg caggcggcat cttccggcac
     4681 gcaggacgcc gccggagaga cggacgacgc agaagctgcg gacggcacgg agcagtccgg
     4741 ggacgatatg ggcgtattca gcgcggagga taccgccaca acggctgtat acgatatggc
     4801 caccggaaaa aagctgtatg agattcccaa ctggatggga ggcctgacag cctgcaacgg
     4861 gacgacgctt tttgtctacg gctacgaggg cggcacggcg gcgtatgatc tggagaccgg
     4921 gcatatgcag actgaggccg ccagcgcggc gggtggagag gattatccgt ccagccttgc
     4981 ggcagacgcg gatggcaact tgtatctgct gtctgaaaaa ggcgtgcgcc gcgccgtgcc
     5041 gggcggcacg ctggcggaga ccgtgatgga aggcagcatg tataccttcg gttccccgct
     5101 ggccgctgtg cgcggcttta cggcgctgcc gggcaacacc ttcgcactgg ccgtgcagac
     5161 ggaggagggc ggccgtgtgc tgcactatgt atttgatgag acggtgagcg ccgtgccgga
     5221 caaggaggtg cgtgtttacg ctctgaacga ttcccccacc gtgcgcgcgg ccatcaccaa
     5281 tttccaacag gaaaatccgg atgtgcgcgt gaattttgag gttggaacct ccggcggcgc
     5341 cagcgccgag gacgcgctgc gcacgctgaa cacagagctt gtggcgggca aggggccgga
     5401 tgtgctgatc ctggacggca tgccgctgca gagcttcatc gacaagggcg tgctggcgga
     5461 gctttcacag gtgatctccg ccgacgggtt gatcccgcag gtggtcgacc ctatgagaac
     5521 ggacggaaag ctgtacgcag tgcccacacg ttttaccgta cctgtgctga tgggaaagcc
     5581 ggagacgctg gaagggctgg acagcctgga ggcgctggcc gcggcggtac aggacggacc
     5641 agaggcgctg gccgccagcg gagatatcaa tgatattttc aaagctgtgc cggaggagca
     5701 gcgcccgctt ctgagcttta ccaccctgcg ggagttgttc gaccagctgt acagtgtcag
     5761 cgcacccgcc gtggtgcggg acggcacgtt gaacgaagat gctctgcggg aactgttttc
     5821 cgtgatgcag gcggtttcgg accgatacgg actgggggaa aaggcgaacg acggcggtat
     5881 tgcgtttatg gtgacgtcaa atgcctccac cgggctggga atgtccgcgg acgttccgca
     5941 gggcgtgatg gaatacagct acggccgggc aaacgcggcg ctgtttacac tcaaggatgt
     6001 gctgggtgca tatatgtccg atgacggaca ggcggcgctt acgccggcgc ccggcatgga
     6061 gcagggcgta tatacaccgc gggtgcttgc cggcattacg gcacagagcg cccagcagga
     6121 gctggctgca cagttcatac aggcgctgct cgcgcccggc gtgcagaatt attcgttggg
     6181 agagggactc ccggtgctgc gctccggcct ggaaaaacag gtggcgtacg tgcaggagct
     6241 ctatgcagaa cgcggccaga cgataggcac ggatgtgccc cgggtggact ggactgcact
     6301 gctgtccggg ctgaagacac ccgtgcggga cgacagcacg gtggccgaaa agctgtatgc
     6361 acaggctgag gcgctgtgca aaggtgaaaa gaccatggat gaagccgtga cgcgagcaaa
     6421 gacggagctt gccacctatc tggcggaaaa gcgggcgtga cctgccgcag aacggcagga
     6481 acaaacccga aaggagcggc cggatgccgt attcagcgat tggaagcatt ggggcgcgga
     6541 agcatttctg gaggaattta cggttccgag ttatcagaat gatcaattaa aggaaaaaat
     6601 atatgccgaa gcaacaaaac gatacagcgg cgaggccgcg ctggaacagg ctgtggcggc
     6661 agcggccgac gccacgcggc tctatttcgc cgaacaaaat gaaccgcgcc gcgccgtatt
     6721 tgttcctcgc gcccagcctg gcggggctgt gcgtgttcgt gctggtgccg tttgcggacg
     6781 cggtgcgccg cagctttacc gacgccatgg gcagccgctt cgtgggggtt gcgaactacg
     6841 cctcggtgct gggcaacagc gcgttccgcc tggcggcggg caatactgcg cgcttcatcg
     6901 gcgtgtgtgt gcccctgctg ctgtgcgtca gcctgctgct ggccgtggcc gtgcgcgccg
     6961 caaaggacgg cggccgtatt tttaaaacaa cctatctgat ccccatggct atcccggtgg
     7021 ccagcatcgt gctgctgtgg caggcgctgt tccacaggca ggggctcgtc aacgccgttt
     7081 tgtcggcgct gggcgcgcag ccggtggatt ttatgggcac agacgcggct ttctgggtgc
     7141 tcatcggcac ctatgtttgg aaaaatgcgg gctatgacat gattctgtgg atggcggggc
     7201 tggacggtat ctccgacagc ctgtatgagg ccgcccgcgt ggacggtgcg ggggcatggc
     7261 agtgcttttg gcgtgtcacg ctgcccgggc tgttgcccac gctgttcatg acggcggtgc
     7321 tcagcctgct caacaccttt aaggtgtttc gtgaggcata tctggtgggc ggagcatacc
     7381 cgcacgacag catctatcta ttgcagcacc tgttcaacaa ctggttcctg gacttggacg
     7441 tgggccgcct gtgtgcagcg gcggtgctca tcgcggcggt gctgctggct gctattctgc
     7501 tgctgcaaaa actgtggggc ggggaggacg cgcgatgaaa agaaaaaatt tgggccgcgc
     7561 ggtgcttgtg ctgttccttc ttttgcctgc gctggcggtg tggtggccgt tgtggttcat
     7621 gggcacaggc gcgctgatgc ccttggacga gctgcgggcc gcgctgggcc cggtgctgca
     7681 gggtgcagag ggcagcgctt tctggcccct gctgccaagc tggcccacgc tccagccact
     7741 ggcggagctt ttgttggaca caccgcagtt tttcaccatg ttctggaaca cctgcgccat
     7801 cgtgttcccc caggtgctgg gacaggtgct ggtgggcgcg cccgcggcat gggcgttttc
     7861 ccggctgcgg tttcgtgggc ggcgggcgct gttcacgctg tacatcgttc tgatgctgat
     7921 gccctttcag gtcacgatgg tttcgggcta tctggtggcg gacgcaatgg gcatcatgga
     7981 tacgttttgg gccatcatcc tgccgggtgc attcagtaca tttccggtat ttatcatggc
     8041 aaaggggttc gatgccgtgc ccatcgcgct gttggaggcc gcgtccatcg acggtgcggg
     8101 gccgctgcgc acttttttcc atatcggcgt gccgctgggt gtgccgggca tcctttcggc
     8161 ggcggtactg ggctttctcg aagcgtggaa cgccattgaa cagcctatga cctttctcaa
     8221 gacaaaagcc ctgtggccgc tttcgctgta cctgccccag atcgcggcgg aaaagctggg
     8281 cctggccatg gtggccagcc ttgtgatgct ggccccggcg gtgctgatct tccggttcgg
     8341 gcagaaatat ctggagctgg gaatccaggc cagcggatta aaggagtaga caacgttgca
     8401 tatcaaagaa aaagtcacgg gcctttgggc ccgcaggccg cgcttgagcg gcacacaggg
     8461 ccgcgcgctg aagggtgtgg cgcagttctt tgcggccatg ctggtctgta cgctgattgc
     8521 gcgcggcacg gcgggcgcaa cgctggccgt agtgcagacg gcgtcccccc ggcgcggaga
     8581 gcttgtacag gggctgacgg cggacgcagc gctctccgcg ggagaggtgc agaacgttaa
     8641 ggcccccgcg gggctggtgc tggaaaaata tttcgttccg gcgggcggca gggtggaggc
     8701 ggacacgcct ttggcacagt ttgaccccga cggtgtgaag gatgcgctgg ctcgcgcaaa
     8761 cacggagctg gcgcgcctgc gcctgcagcg ggaaaagctg ctggcagggg acacggcgga
     8821 cacctcgtcg ctctcttccg cctctaccca gcgggacagg gcacaggcgg attacaacgc
     8881 ccagaaagcg cagaacgacg cggccgtggc cgctgcccgg aacgatgcgg atacggctca
     8941 aagcgccttg gatgcggcaa atgccgcgct ggcggaactg cgggcacaga cggacccgcc
     9001 cgcctctgag gaagcgttgg ctgcggcgca ggcggcggca gacgccgcgg ccgcggcgct
     9061 ggctgaaaag caggcggcgc tggctgccgc gcagagcgcc gccgataccg ccggggaaaa
     9121 cgcggcgcgg gctttggccg acgcgcaggc cgcgctggac acagcggcaa aggcgtatga
     9181 aaaatcaaag caggaaacgg gattccagaa ccgtcaaaac aaccttgacg cccagacggc
     9241 cgcgctggac attgaaaaac agcaggctgt tgtggacgcg ctggcggcgc tggcggaaaa
     9301 cgacggcgtt ctgaaagcgg gatcggcggg tacggtggag acgctgacac tggaaccggg
     9361 cggaaccgtt acggaggccc cggtggccgc gctggccccg gcggacgcag gcatgacggc
     9421 gacattcacg ctggactcgg gaaaagcgga aaagctggct gtgggcgccg cgctgacgct
     9481 gcggcagggc ggcacggctg tcagcaccac ggtgcgggcc atctccgcgc cggatgaaaa
     9541 cgggcgcgtt accgtaacgg cggatgtacc tgcggatgcc ggcctgcgca agacgcagcc
     9601 cgtgactgcc tcggcggagc tgtcccgcac cacatacgaa atggtgttgc cgcctgaagc
     9661 ggtgcgcatg gacaacaaag gcagctacgt gctgcgcgtc gagcagacgc agaccatcct
     9721 cggcctgcgc aatgtgctct cccgcgtgga tgtgacagtc ctggaacaaa gtgcatccgg
     9781 tgttgcggtg gaagggccgc tgtcgcccca ggatgttttg gtaaaaagca gcgccagacc
     9841 cgttgcagcg ggagacagcg tgcgggtgga gcagccatga ggcgcgtgcg gtattatctg
     9901 ggcggtgcgc tgttctgggg cgcattcctt gcggtttgcg ttttgctgtt ctggaacgcg
     9961 cagcgtctgg cgcgggccgc gggcgggatt tccctgcggt acggcgaagc gctggagccg
    10021 cgcgttgtgg cttcggcgct ggagtatgcc cgggacgggc ggaacaatgc ggcttggtcg
    10081 cccacattct gggctgtgcg gcagggagtg gcctctgcca acgggcggga ggagaccgtc
    10141 cggatgctgc gggtgtgcgg cgatgcagaa cttctggcaa cggagcctct tttatacggt
    10201 gcgctgcccg gcccggcagg ccagggcgga tgcgcggtat ccagcgcgct ggcctggcag
    10261 ctttggggag cgctggatgt gacgggcctg gagctggagc tggacggcga acgttatacg
    10321 gtgcgcgccg tgtttgaaag cggagaggcg cgcgctgtga cacaggaggc ggcggacagc
    10381 cgcactgcag agtacgccaa tgtggaactg acggcgatgg aaaccgctcc ggagggcaca
    10441 gaggcggcct tggcctttgc cggtgccagc ggcctgggta cgcccgccca tgtggtggac
    10501 ggcccggcgc tggccgggct ggcctgtgcg ctgtgctggc tgccgttggc gctgtgcggc
    10561 gcatgcacgc tgtggcggct ggcacgcggc gcacggcggc ttcggccgtg gcagcggcag
    10621 acgctgctgt tcgctgcgct gctgtttttc gcggcgctgc tgcccgtgct gctggcaatg
    10681 gtgccggggg cctgggtgcc cacgcgctgg agcgatttcg gcttttgggc ggcgctgggc
    10741 aaaagcgcgg cagcgcgggt ggacgcgtgg ttcgctctgc cgccccagca caaggacata
    10801 gcggtaaagt ggctgcttct ggagcagggc gcgctgtgcc tggcgggatg cctgtttgcg
    10861 ctgcgccgcc cacccgcgcc gcggaatgaa aaagcggttg aagcgtaaaa gtcaaagccg
    10921 aatttacaaa atatttactt ccggtagaaa tattgaccga tttcctgtat aataggcaca
    10981 cagaagtaaa atccgccctt gaaaggggag ccctatggaa tgggagatcg gtgcggaatg
    11041 cctggcctgt gtcctgatcg tactggtgct gtgcttttcg cgtgaaaaat tttatacgca
    11101 gatgccccag acgcgcttat actatgcgtg tctgggtttt gcgctttttt ccactctgct
    11161 caatatcgcc acagtggtgc tgatgggcgt gccgggcctg ctgccgcgct ggctttgcta
    11221 cacgctcaat atgctgtatt ttacgttctt tccgctgctg gaagccgcgc tcacctacta
    11281 tatggcctat ctcatccacg gccgcggccc gtgcttcacg cgcgttgcca tatgcctcgg
    11341 cgtcatgctg gccgccgcat tggcggtggt gttcacaaac ccgttcacag gttggctgtt
    11401 ttatctggac gcggcgggca catatgtgcg cggtccgtac aaccggctgc tgtatgcgct
    11461 gctggtggcg tgctgcttgt tgctggtgat atgttatata aagcagttcc gcacggcgtc
    11521 gcgcgccatc caccgaatga tggccacgct gccccttctg gcgatggtgc tggggacggt
    11581 gcagtatctg ttcccgcccg tcatgatgac gggctttatc ccggcgtgca ttttgctggt
    11641 gctgtttttt aatttccaga gccagcgcat caatacggat tttctgacgg gcctttccag
    11701 ccgcagcgcg ctgtggtatg cggcgggcac gtgccttcgc tcgggccaat ccttttattg
    11761 cgtagcggta tgcctgcgcc atttcggcga cgtaaacaaa cagttcgggc acaccggcgg
    11821 cgacgcagtg ctgcgccagg tgagcgcata tctggaagcg ctgggccgcc gggccgtcgc
    11881 ctgccggttc agcggcgtgg aattcgtgct gttattcccg ggcatggatg ctgcgggcta
    11941 tgcagtgctg gaaaaggaac tgtcagagcg ttttgcagcg ccctggcagg cggggggcgt
    12001 gtgctgtcgt ctggacgcgg gcgtggcagg catcgcctgc ccgcggtttg gcgacacagc
    12061 cgagcgtctg acagctcatc tggaatacgc catccagcag ctgaaattgc ccggcgcacc
    12121 ggaggcgctc tcgtttgaca cagagatgga acaacggttc tcccggcgcg tcgcgctgcg
    12181 ggatgtggtc gtacgcacat tgagcgggca ggcggcgggt gtggcgtacc agcccatcta
    12241 cgcgctggat gcagatactc cctgtatggc ggaggccttg ctgcgcctgt gcggcgcaga
    12301 tgggacgccc gtacccacgg cggatgtggt gtccgtggcc gaggagatgg accttatcgt
    12361 tgcgctggac tggatgatgc tggaacaggt gtgcgcgttt ttcggtgcac accgcgagct
    12421 ggatggatgc gcggtctctg tgaatttttc ggcccggcag tttcttgcgc cggatgcgga
    12481 acgtcgtgtg ctggatacgc tggagcgcca cgggcttgcg cccgctcggc tgaagctgga
    12541 attaacggag cgcgtgttgg ccggagatat ccggcgtgtg cgcgccgtga tggaggcttt
    12601 ggccgcccgg ggcgtagaat tttatctgga cgatttcggc accggatatt ccaatctctc
    12661 cagcgtggtg tctctgccgc tgcagtatgt gaaggtggac agaagccttt tggaagccgc
    12721 gacgaatggc ggcgggggag cgcttttgct gcgcgcggtg gtggaaacgt tccgggccat
    12781 gggccgaggc atcctgctgg agggtgtgga aacgccggag cagtatgcgc tggcgtgcac
    12841 gctgcgtgcc gaccggctgc agggatacta tctggcgcgc ccaatgccgg gagaggagct
    12901 ttgtgctctg atgcgcagcg gcgcgcggcg cacgcgcaga acctgaaacg gctctggctg
    12961 cagccgctga aatgaaaagc gccctgccgc gtagaaatgc ggcagggcgc ttcggtttta
    13021 tcaaaaggaa aaggcggcgt cagcggcgtg cttccaccag agccttggtg tctcgggcga
    13081 tcatcagttc ttcgttggtg cagacgacca gcaccttcac gcggctgcct ttgccgctga
    13141 tgtccaggat atctgaggtg cgctgctgat tctttgcggg gtccacggag atgcccagga
    13201 accccatatt ctcacacacg ctggcgcgca cgcggttgtc gttctcgccg atgccgccgg
    13261 taaacaccag gcagtccagc ccacccatag cggcggcgta ggaacctaca tatttcttga
    13321 tctgatacgc aagcatctcg gtagccagtt tggcgcgctc gttgcccgcg tcggcggcgc
    13381 tcgtcacatc gcgcatatcg ctggaaacgc cggaaatgcc cagcaggccg gatttcttgt
    13441 tcagataatc agccatttcc tggccgtgga atcccagctt ctgttccatg taggtgacga
    13501 cgctggggtc gatgtcgccg cagcgggtgc ccatcagcac gccggccagc ggggtaaggc
    13561 ccatgctggt gtccacacat ttgcctgcgt tcacggcact gatggagctg ccgttgccca
    13621 gatggcaggt gaccattttc aggtcggaat cgttcaggta ctgggcggcg gccatgctca
    13681 cataacggtg gctggtgcca tgggcgccgt agcggcggat ggaatatttt tcatacatct
    13741 catacggcac gccgtacata tacgcctttt tgggcatggt ctggtggaag gtggtgtcga
    13801 aaacgacaac gttgggcacc ttttcgccga acacggcctt ggcgctgcgc aggccctgca
    13861 catgggccat gttgtgcacc ggggccagag gggcaatctc ctcgatcttg tcgatgatgg
    13921 cgtcggtgac gatctcggac ttggtgaaaa actcggcgcc ctgcaccacg cggtggccga
    13981 tggcagagat ctcgctcttg tccttcacga cggcgccctc gccgctggtc atcatttcaa
    14041 cgaccttcat aaacgcttcg gtgtgggtgg ggaagggcac ttcctgttcc cactcgccgc
    14101 cgttggcctt gtgtgtgatc ttgctgccct cgatgccgat gcgctcgcac aggcccttgc
    14161 acagcacgcg ttcgccctcc atatcgatga gctgatactt cagcgagctg gaaccacagt
    14221 tgatgaccaa aactttcatc cgatatttcc tcccattcac aaaaaagcgc agttatgctt
    14281 tttaaaatcg ctccattatt atataatgat tataaagcat tttcaagcgc acggccggaa
    14341 acgcgcaaga aaaagcgaga aaaacggcca agattcgcaa tttatgcgtg cacagcggcc
    14401 gaaagaagcg aggcagccat gagaacagcc ggtatcatcg cggaatacaa ccctttccac
    14461 aacggacacg cctggcagat agcggagctg aaaaaacgcg gctttgaggc ggtggtgtgc
    14521 gtgtgcagcc cgggcgtggt gcagcgcggt actgcggcgc tgtttcccgc ccgggtgcgt
    14581 acccgggctg cgctggccgc cggggcggac ctggtgctca gcctgccggc accctacgct
    14641 acgctgtccg ccgagggctt tgcagcggcg ggtgtggcgc tgctgagtgc gctgggctgt
    14701 gtggatgctc tgtgcttcgg cacagagaca cggcggctgg aggatatcgc ccaaaccgca
    14761 gcgctgctgg aaagcccgga ctttccagca gcgctgcgcg ggcagctgga cgcgggcctg
    14821 ccgctccccg ccgcccgcgc ggcggcggcg gaagcgctgt gtccgggagc aggcgcggtg
    14881 ctgcgcgcgc ccaatgacat cctgggcgtg gaatactgca aggccctgct gcgccagcac
    14941 agcgcacttg tgccccttgc gctgccccgg cggggcgcgg cccacgatgc cccgctggat
    15001 gcggcgccgg ggcggtacgc ttcggcttcg gcgctgcgcg cgctggtgcg ggccggggga
    15061 gcggcggctc tcgcgccgca tgtgccgcca gcctgcctgc ggctgtatat gcaggccgag
    15121 gccgagggcg acattctgga cgggcgcgcg ttcggtattg cggtgctgtc ccgcctgcgg
    15181 gctatggatg cggcgggtct ccgcgccgtg cggggcgcgg ccgagggcct ggagaaccgg
    15241 ctcgccgccg ccgttcaggg cgcaggcacg ctggacgagc tggtgcaggc catgcggaca
    15301 aaacgatatg cgacggcgcg tttgcgccgt ctggtgctgg acgcggccct gggatacacc
    15361 ggggatctgc ctgcactgcc gccctatctg catgtgctgg gagcaaatga aacgggcctt
    15421 gcggtactgc gctgcgcacg tcaggcggct ctgctgccgc tgtctcattc actggcggat
    15481 ctggcggccc gaagcgcccc ggctgcgcgc gtggcagcgg cccatgccgc ggccgaggac
    15541 ctgacggccc tgtgcctgcg ccatcctgcg cccatgggcg ctgcatatac cgcaaaaccc
    15601 gtgtttccca caagcggggc gaaataagag ccgggaaaag gaacgcgcag cgcccttcgg
    15661 ccttccgcga tttttggagg atgcacgccg gatatgggaa tagtatgcac tgttcgggag
    15721 cggatcgtgc attctttcaa gggaaaacgc cggacgggga aaatacaaag gagctgaaac
    15781 acatggagat cattcgggca gggatgcagg aagcggaccg ggcggcggct ctggcgcagc
    15841 tgctgtggcc gggccacacg caggctgagc tggaacagga gctggcggga tatctgcagg
    15901 ggccggaggg tgcggtgttt cttgcacggg aggacggcgc ggacgtgggc tttgcgcagt
    15961 gcggcctgcg gcgcgactat gtggagggaa cgcacaccag cccggtgggg tatctggaag
    16021 gcgtgtttgt gcaagaggaa taccggggcc ggggcgttgc gacggcgctg ctgcgcgcct
    16081 gcgaggcgtg ggccggggaa aaaggctgtg ccgaatttgc aagcgactgt gggctggaca
    16141 acgccgcaag cgccgtgttt catctgcgcg caggctttgc cgaggcgggg cgcatcatct
    16201 gcttcacaaa gcggctgtga ggaacgtgga gagtgagcct ttgcgggatg cgtgagccaa
    16261 aaattaccgc cgctgctggg caagatttgt aaagagaacg tgcagaaacg caacatttgg
    16321 cttgcatctg cggctttttt cagtacaata gaaaatgagc gaaaaatcca taatgtgaag
    16381 caaatatcaa tatctgataa acatttcaca agtttgagag gaaaaggaga aacgccatgt
    16441 cgatccatgt gctggacgaa gccaaccgct gtttgcactg taaggcgccc cagtgccaga
    16501 aagggtgccc catccatacg cccatcccgc agatcatcgc gatgctgcag gagggcaggc
    16561 tggacgaggc agggcgcacc ctgtttgaaa ataaccccct caccactgtg tgcagcctgg
    16621 tgtgcaacca tgaaaaccag tgcgagggcc actgtgtgct gggcaaaaag ggagcgcccg
    16681 tgcatttttc cgccattgag aattacattt ccagcacgta cagcaccaag atggtaaaag
    16741 gccccgcgcc atccaacggc atgcgggtgg ccatcgtagg ctccggtccc gcggggctga
    16801 ccatcgcggt catcctggcg cggtacggct atcaggtgac catttttgaa ggcaaggata
    16861 agattggcgg cgtgctgcgc tacggtattc cggatttccg cctgcctaag accgtgctgg
    16921 acgatttcga ggaccgccat ctcatccgaa agggaatccg tgtgcggccc aacaccacca
    16981 tcggcagcgc catcggcatc gatgatctgt tccgtgacgg ctaccgcgcc atttttatcg
    17041 gcacgggcgt atggcagccc cgcgcgctgc acattaaggg cgagacgttg ggccatgtgc
    17101 attttgcaat caattacctc aacaatccgg acagcttccg cctgggtgag cgtgtcatcg
    17161 tcatcggcgc gggcaatgcg gccatggatg tggcgcgcac cgcgctgcgc aagggcgtgc
    17221 ggcagctcac ctgcttttcc cgcacgccgg aggtggccgc cagccagcat gagttcagct
    17281 acgccacgct ggagggcgtg aattttgaat attgcaaagc gcctgtggag atcacggacg
    17341 agggcgtgat cttcgtcgat gtacgcgagg atgagaacgg cgaattcatc gaggcaccgg
    17401 gcacggagaa gctgtacccg gcggacagtg tgatcgtgtc catcagccag ggcccgcgca
    17461 accgcatcgt cagcactacg aagggaatcg acaccgaccg gcgcggcctg ctggtgaccg
    17521 atgaagaggg ccacaccacg cggcccggcg tgtttgccag cggcgatgtg gtgctgggcg
    17581 caaagacggt ggtgcaggct gtggcatact ccaagcgggt ggccgaggcc atgcactctt
    17641 atatgcaggg gatacccgcg cagggacggg ccgcggggcc ggacggcgcg cagcggtagt
    17701 gcctgtcggg cctctgcgga aaacaaacgg aaaggtccgt gaaggcgttt gccttcacgg
    17761 accttttgca tacgggtatc tgcggcggct ttatttccgc cgggagcagg ccgcaccgca
    17821 agcggaacaa ttgccgccgc agctctttcc cctgcgtttg ttgtcgatgg ttttgtacaa
    17881 cgccagccca aaggccagcg ccaccagcgc aagcagcgcc caatcgagaa gatccatcct
    17941 aaaatacctt tctgttgaaa cggagataca tgcatcagcg gagaaacagg ctgccaatct
    18001 ggtacaccag gcaagcgcac acccaggcca ccgcacactg gaacagcgcc acgcacgccg
    18061 cgccccagcc ggagcgcatt tcccgtttga ctgcggcgat ggccgccaca cagggcgagt
    18121 acagcag
//
LOCUS       DAWBSB010000015        17644 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_845_length_17644_cov_3.526181, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000015 DAWBSB010000000
VERSION     DAWBSB010000015.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 17644)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 17644)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17644
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_845_length_17644_cov_3.526181"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3000..3009
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3676 a         5236 c         5299 g         3423 t
ORIGIN      
        1 gtggtatgca gaaagcgaac caaacctttg tatgcggtcc ggttcgcctt cgcacatccg
       61 ccgcgcagcc ccgccgtcag cgggaccgcg gcgtccggtt tgcggtttat gccatacccg
      121 cgttggaatt ttccacgtag gcgtttacag cagcggtgac gtcgtcgcca ttggtgacag
      181 cctgcagcat gttccaccat tcggtgcgca tgttggcaaa accgtccata gtgttgtaat
      241 acggagcgta atatttcgtc cagccggaca gcagcttata ctcttcattt cccgcataca
      301 ggtcgccgaa actggcgcgc acgggaaatg cgccggtgcg cacaacgttc ttcgggcccc
      361 aggtctcgtc gtcgcataca aatttgataa actctttcgc cgcggcagct ttcgtgtcat
      421 cgccgttgtc gaacacacag aagccgttga ccagatactc cagcgcgggc acgccgtcgt
      481 cggaggggaa cggcagggaa agcgtggtga tagcgttctc atccagcgtg gcctgattca
      541 caagctgcgt ggaggtaccc cagcaaagtg taaaagaggt gctgcctgtc ttgaacagct
      601 cgatatcgct gcctccgttg taggcaacgc cgttgcccaa aaggccgtcc tgaatccact
      661 tctgcgcagt ttccaggccc ttgacgccag cttcgctgtt catggtatac tcggtcatgt
      721 ccgcattggc aatttcggct ccgtacagat tgctgatgaa ggcccgggtg ccctggtccc
      781 cgccctggcc ctggcagaac agtgtgccgg gattcacacc ggcctcgtgc agagccttca
      841 gcgctttttc aaagtcctca gtagtccagg tgcggtcgcc ctcctggttg acaaactcaa
      901 gagcgccggc ttcttcccac tgcttctgat tgatgaccat atagaacgga gcggagctga
      961 tgggatacat gtaggcggtg ccattgccct tgcatgcatt gaccagcgcg tcgttattca
     1021 catctttgac gaattcatcg gtaaacagat cgtccagaga caccagcttg ccattcttgc
     1081 cgtattcgat gatgcggccc ggggcgtcga acagcacgtc gggggccgca ttgccctcga
     1141 tggcggccgt gatggcgtcc ggcccggagg tgaagtcgat ggtctcgagg ttcaccttga
     1201 tgttgggata cttttcgttg aacgcttcaa tgatcttctg ctcgtaagtg ccggcgggat
     1261 catcggcatt ttcctgccca aaggtaggga acgcccacca ggtaatctct gcggtcatct
     1321 gggtggggtc gtccgacggg gaagcggaag aagaagcggc cggggccgac gtagagacgg
     1381 gtgcggaaga ggatgtgccg cttgcattac agccggcaaa aatgccgacg gacagaacca
     1441 gcgcgagagc gacggcgaac agctttttca tagtggtcct cctgtttttg atatattggg
     1501 gacggcgcgc accgcgtacc ggtacctgcg gggcgcgtcc tgtcctgctt cgtcctcata
     1561 gtaccattta tgaaaaacat tttcaatctg cttttgtaat gaaaatcttt tcgttttttg
     1621 tgcatcgtgc caaattctta caatttgatg aaaagctctt gtgtgttttc ctttcgtatg
     1681 cttataatta aagaaagcat aaatgaaaaa gattttcatt tttaaatctg aaaaggaggc
     1741 gccccgtgga atataaggac gtattggata cgatgacggg tgaatacaac aacctcacga
     1801 aatccgggcg gaaggcagcg gactacatct tcgcgcaccg tgtggaggca cagtacatgt
     1861 ccatcacggc gctggcggag gaatgcggcg tagccgaggc cacgatctca cggttctgcc
     1921 gcacgctggg cttttccggt tacaatgatt tcaagctggc ccttgcaaag gccagcggcg
     1981 ccccggcggc ggatgacgag gtcggcatct atggaaaggt cctgccggag gacagcatta
     2041 ccgacatgtg caaaaagctg tatgccacga acgtggcggc cattggccag acgctggcgc
     2101 agatcgacga ggagaatgtg ggccgggcag tgaaatatct ttccgccgca cagcgggtct
     2161 actgcttcgg acagggggga agcctggtca ttgccatgga ggcatgggcg cgtttcatta
     2221 cagccgcacc ccagttccag tgcatcgagg actcccacat gcaggccatg gccgcggcgc
     2281 tctccacgcc ggacgatgtg atcctgtttt tttcctattc cggcgcgaca aaggatatgc
     2341 tggatgtgct gcgcccggcg cgcagacgcg gcgcaaagat catcctggtg acgcattttg
     2401 caaagtcgcc tgccgctgcg atggcagatg tgaccctgct gtgcggctcc aaggaggggc
     2461 cgctgcaatc cggttccgtg gctgcaaaga tgggtctgct gttcatcatc gacgtactgt
     2521 tcaatgaata ttgccgcacc aatcccgccc tgacggaggc gaaccgcgag gctacggcag
     2581 gagccatttc agcaaagctg ctgtaagcac agaacgaaag ggatgtgact gtgatggatt
     2641 atgaaaatat caccgagcgc cgtatacttg tatttggaga ttcaaacacg tatggatacg
     2701 acccggagcg ggacggccgt tatggggaaa cagagagata tccgtgccgt atacaggctt
     2761 tgctggggcc gggctggacc gtgatcgagg agggcctgcc gggccgtacc gcggtgtttg
     2821 acgaccccgt caccgaaggg ctgtgcggcc tttcctacct tacgccctgt atgatgagcc
     2881 atgcgccctt ggatacactg gtggtgatgt tgggaacaaa tgatacgaag gaacggttcg
     2941 gatgcaatgc ctatctcatt gcacagggca ttggacgcct gttgaaaaag gctgccgatn
     3001 nnnnnnnnnc cgataccgat gcatggcgtg ataaacccga tatattggct gtatgccctg
     3061 cgcctattgt gcccgcatac gagagtctgg tcttccgcaa cgctttgggt ggcggatgtg
     3121 cggaaaaggc tgccgctctg gcacaggaac tggagcccgt ggttctgcag ctgggcgcgc
     3181 gctttctgga cgcagggcgc gtgcccggcg tggaagtgca tccgctggac ggcatacacc
     3241 tgacgcggag cgcgcatgcg gcattggccc aagcgcttgt agaggtagtg aaaacgtgat
     3301 aaaatgaggg cggacaagat ccgtccttat tttttgcggc cggctgcgga cgtgttattt
     3361 ttacagcgtt tttgttttgc ggacacgtct tcatgctgta tgatggatct gaaaaacgat
     3421 cggaatgaaa aagaaacgga gtattcctgt gaaactggac aaactgactg tactgaaaat
     3481 cctgggcatc gcgggtgcgg cgatattctt ttactgggcc ctgcaaaacc tgtcgctgct
     3541 ggcggacgcg gcgggctggc tgctcaatat cctgtggccg cttctcctcg gcttctgcat
     3601 ggcgttcgtg ctgaacatcc caatgcgctt ttttgaacgg caccttttga agaacccaaa
     3661 aaacaaacgc gcggcggcgc tgcgccgtcc gttgtgcatc ctcctctcgc tgctggccat
     3721 cattgttatt ttgacggcgg tgttcgcctt ggtggtgccg gagctgctga gtgcgttcgc
     3781 ggtgctggga gaggccatcc ccgagttttt gacggccgcg cagaagtggg cgctggaagg
     3841 ggccgaccgg ttcccgcagg cggagcaatg gctggcttcg ctgcagatcg actggaatga
     3901 aacaggcaaa aagcttctga gctacgttac cgaaggcgca ggcaatctgc tgggcggcac
     3961 ggtatcggtg ctgtcgggca tcgtgggcgg cgtgttcaac gccatcatgg cgttcatctt
     4021 cgcgctgtat atcctgctgg gcaaggagaa gctgaaaagc cagacggccc gtgtgtgcgt
     4081 ggcgtttctg ccgcgcaaat gggcggacag cctgttcttt gtggccaata tggccagccg
     4141 cacgtttgcg aaattcgttt ccggccagtg cacggaggcg tgcattctgg gcacgctgtg
     4201 ctggctgggc atgtcgctgc tgcggctgcc ctatgcgccc atgatcggcg cgctggtggg
     4261 cattacggcg ctcattccca tcgtcggcgc ctgggtgggc ctcattgtgg gcgcattcat
     4321 gatcgggatg gagagcctga cgcaggcgct gatcttcgtg attttcctgc tggtgctcca
     4381 gcaggtggag ggcaacctca tttatccccg tgtggtcggc tcctcagttg ggctgccgcc
     4441 catctgggtg ctggccgccg tcacggtggg cggcggcctg tggggcattg cgggcatgct
     4501 gtttgcggtg ccggtgtgca gtatcctgca cgcgctgctg cgcttgggcg tgcaccgccg
     4561 cctggaggcg caggctgctg ccggggcctc tgtgcctgcg ccgacgccgg aggagaaaat
     4621 gtgactgaac cagaaaagcg ccgttccgcc ggaacggcgc ttttttcttg cgtatatact
     4681 acaagaatgg tatactataa cccatcggcg gcccgtccgg gccggagttt tggaataaaa
     4741 gaagaaacgt gccgcgggaa aatcgcccgc cgcgcgggag gaggggtttg aatgggcctt
     4801 ttggcggacg cgaagtccat gctgaaaaaa gatccggctg cacgcagcct ggtcgaagta
     4861 atgctgctgt atccgggctt tcacgcgctg gtgttctacc gggtgtcgca ctggttttac
     4921 aagcacggac ggttgttctg tgcccgtatg gtcagccagt gggggcgggg cttcaccggc
     4981 atcgagatcc atcccggggc caccatcgga gacggcctgt tcatcgacca tggcgcgggc
     5041 gtcgtcatcg gcgaaacggc ggagatcggt aacaacgtca ctctatacca cggtgttacg
     5101 ctggggggca ccggaaaaga tgagggaaag cgccacccca ccatcggaga caacgtgctc
     5161 atcggcacag gctgcaaggt gctggggccc atcaccatcg gtgcgaacag ccgtatcgga
     5221 gccaacagtg tggtgctgtc ctgtatcccc gccaatgcca cggccatcgg cattccggct
     5281 tgcgtggtgc gtgtgaacgg cagcaaggtg gcgcgttcca gcgaggagct gaaccagcgg
     5341 gactatccgg acgtggtgca cgcggacatc aaggcgctgc agcagcgcat cgcggcgctg
     5401 gagcgcgctc tggcggaccc gcaggctgcg cctgcagaaa aacggtaaca aaaaagcgcc
     5461 ccgttcagcc ggaacggggc gttcttttat ttttgcgctt accggaggct tacagcgcgg
     5521 gcttcagcag atagaaatac gccaaaacca gcgcgcccag accaatgcgg taccagccga
     5581 acaccttgaa atcatgcttt ttcacatagt tcatcaggaa ccggatgcac agcatggaaa
     5641 cggcgaacgc cacggcgcag cccgtgagaa gaattcccac ttcagagccg gaaaagccgc
     5701 cgccgtcggc caggaacttg acgaccttga cgagactggc tcccgccatg gtggggacgg
     5761 ccaggaagaa cgtgaactcg gccgccaccg tgcgtgaaca gccgatgagc atcgcgccca
     5821 ggatcgttgc gccggaacgg ctggttcccg gcaccagcgc caggaactgg aacaggccga
     5881 tgaacagagc catgcggtaa ttgatctgtc caatgcgccg cacctggggg cggcgcttgc
     5941 ccacggtgct ttccataagc aggaagaaca cgccgtacag gatgagcatg gcggaaacga
     6001 cgggcggatt gtgcaggtgc tcggtcatcc aatcgtccag aggcaggccg atgacggcgc
     6061 tgggcacaat ggccaccagc acgtggaacc acatgtccca cacgtccttg cggcagtagc
     6121 ggtccaccag gccggacaca ccggaaccgc aggcgtcccg gttgatgcgg aacggccaca
     6181 gtttgctcca gaaaatcact accaccgcaa ggatggcgcc gaactgaatg actacatcga
     6241 acatttcctt aaaagcatcc gatgtggaca tatggatgaa ttcatcggcc aggatcagat
     6301 ggccggtgct ggaaatgggc agccattcgg tgatgccctc gatgacgccc agcagcgccg
     6361 cgaaaagata atccagcaaa ataagtcccc cttattccgg gcgcacagcg tccgtggtgc
     6421 ggggcgcgcc ccgccgtaca gtatatgcgt acgtcacaca tccaagacca gccccaccgg
     6481 gcagtggtcg gacccgtaca cgtcgctgta aatgagcgcg tcgccaattt tttcgcgcaa
     6541 acgttcgctg acgatgaaat agtcgatgcg ccagcccgcg ttggtctccc gcgcgcggcg
     6601 gaaatagctc caccaggtgt agcggcccgc ggcgtccgga tacagcgcgc ggaaggtatc
     6661 cacaaagccg ctcattaaaa ggtcggtcat tttctggcgt tcctcatagg aaaagcccgc
     6721 cgaatggatg ttctgcttgg ggtttttcag gtcgatctcc cgatgggcga cgttcaggtc
     6781 gccgcaatag accacaggtt tttccgtatc cagcgcgcac agataagcgc gcagatcatc
     6841 ctcccactgc atgcggtagg gcaggcgcgc cagttcattc tggctgttgg gcgtatagac
     6901 gttgacgagg aagaactgct cgaactccag cgtgatggcg cggccctcgt gactgtgctc
     6961 ctctttgccg atgccgtagc gcacggagag cggttccatg cgggtgaaaa cggctgtgcc
     7021 ggaatacccc tttttctcgg cactgtacca gtattggtga tagcccggcg gcgaaaattc
     7081 ggcctggccc ggctgcatct tcgtttcctg aaggcagacg acgtccgcgc cgaagccctc
     7141 gaagatgtcc gcgaagccct tgccgaggat ggcccggaaa ccattgacat tccacgatat
     7201 caatttcaaa acgatatacc tcgcatattt ctttcaaagt tcgggacttt ccgtcccgga
     7261 tattgcacag tatagcacaa aatagccccg ctgcataccg ggatgcggcg gggcgcgcgt
     7321 tattttccgt tttttgcacg gaacggaccg ctcatgtcaa aaaggtgacc acaagaataa
     7381 gcgccgcgca cagacctgtg tatgccagca tgcccggcgt gcgcagcgca cggccaaagc
     7441 tgtgggccgg gcgctgcgcc ccctctcctt ccctgccgtc cgcgggtgct gcaccgggaa
     7501 accgccagcg cctgcgcgcc gcgatcacgc cgcccgccgc aatgctcaca agcaccagta
     7561 tccccacggc agccgtgccc caaatccctc cgtattgtat gaaaaggggc gccgccatcg
     7621 tgccgatggt gttgatggca acatgcagca ggatggtata ccgcagcttt cccgtgtggg
     7681 cgtaaatgta gcagaagacg gcgcccagca caaaggcata caaaagctga gacagattgg
     7741 cgtggaaaag cgcgaacagg aacgcgccca ccagtacata ggtgcggacg ccgtatccgc
     7801 cgattttatc gtacagcagc ttgcggaaca aaaactcctc ccccacgggt gccagcacgc
     7861 aggcgaacag aaacgtggcc agcgggctgc tgttcatttg catcagggca agtgggttct
     7921 gcaccgcgcc gcccttaagc attcccagca gagctgtgat gccgagggag acgaaattga
     7981 acagatacgt cacgcccaga cacagcacca gccagcgcag ccctcctccc gccgtcagcg
     8041 cggcgctgcc cgccgtgccg ggcgtgccgg gcacgagttt ttgcagcagc aggataaaaa
     8101 cagggacggc aatgcaatac agcggcgcat aggagagcgc ccacagcccc cagcccgtct
     8161 ccagcaggct gcccagcagt gtcatgccgg ccagctggag cgcctgtgtt accagcatca
     8221 gaacaaagta gcaaagcccc acccgggaca tggtgttttt gaatgacaaa ccgttctgca
     8281 tcacagatgc cctcctcttt ttcagcagcg gcatgcggcg cgcttttgac actccgccac
     8341 agagccgccg cactgtgctt atgctgtctg gcgcacgctg ccccgcagca gcagagcggc
     8401 gcctgaaacc gtggcggctg taagaagcgc aagcgccaga agcacccgcg ccggaccgcc
     8461 ggggatgaat gccagaacgt ccaccaccgc gcccagcgca cggcccagca atgtgtcgcg
     8521 tcccgcgccc tcggccagcc gtgtgaaggt gtcgccgaac aggaaaacag ccatatacgc
     8581 ggcccacagc agccacagcg cccgccgtcc ccagcgctgg atacatgcgc ccagaaacag
     8641 cccggtgcag gcaaggacga gaactgccgg caaaatcacc caggcccagc gcagtgcctc
     8701 cggctgcaga aggtccggcc cgtcaatggg catgccgcgg aaaagcagca gatacagccc
     8761 gcggtccagc agcgtcaaaa ccactatgcc tccgacgagg cacagcagct gcaggcacac
     8821 ggtgacgaac atcccaaaca gaaaacggcg ccgtgtacag ctcatcgtca gcgcctgctc
     8881 gtagccgcag gagaaatagt acagcgcaaa aataacgccg aacatcacca gcatgatcgc
     8941 tgcaaataca gtgccaaggg aaaaatactc ctcctcatga gcgaaataca tgatacacag
     9001 gctaagcgcc tcgccaagca gccaggctcc gcccaaaaca gccagcgatt gccacaggct
     9061 gttccggttg agcaggaatt cccgtttgat cgattttaac atatatgctc acccctttac
     9121 cggtatgcgg cggcacatga gccaatccag ccagcccaac agcacggcca aaacgatggc
     9181 gctgccgccc agcgcccacg cattggaaag aaagcgcagc atcgcgtcca gcatgccgcc
     9241 cagagctccc gccgcacccg cgtagccgat gatcccgccg atcaccgcac atccgccgac
     9301 gatcagtacg aaaaatacga tcatgcccac acgcccaaag cggaggctga caaaacccag
     9361 cgcttcacca acggacgcgc cgaggagcag cacggcaaac agccccacca gcatcgtacc
     9421 ggtgaacagg gcgtccacgc cgaacatcca ttttactgcg agctggatca aaagcaggca
     9481 tacgccgatg cctccggcca gcatcacctt ggccgcctgt gctccggcga agaagccccg
     9541 gcgcgtaacg cccatggaaa gaggcagcgg catgtaagac tggatcaggc tggcctgcag
     9601 cacaaagggg aacatcatcc cgcaggcgaa ggccatgccc aggatacgcc aacccgcgct
     9661 ttcaaattcg gacagcggcg tggaaacggc gtaaaatgca atgaacagtg cgccgacgcc
     9721 caacagcgcg ccaatgaact gcactaccag caggctcaga tattttacgg tacgcagcat
     9781 cgttattccc cttccttatg gccgcccgtc aggtagacga acaccttttg aagcgtcacg
     9841 ccgctgatat ccacatccag cccgtctgcg ctgcggcgca gcgtatccaa gctgccgcgc
     9901 acgcaggccg tgcggctgcg gcccaggccc tcttcgtgga tgacctgcag cccggcgcag
     9961 gcgcgggcca cgtcctcgtc ccggcctgaa acatagtgga actgggccac gaagctttcg
    10021 cagttgcctg tctcgatgac acggccgttg tcgataaaaa taatatcctc aaacacgccc
    10081 gcggcttcct cgatgatatg ggtggagatg atgaaggtgc gtcccgtttc ggcgtaatcg
    10141 gccagaagca catcgtagaa ctgttcccgc gccaccacat ccaggccggc caccggctcg
    10201 tcgagaatgg tgatgggcgc gcggctggcc agcgccagaa cgatggtgac catgctcatc
    10261 atacccttgg agagcttgta gattttcttt ttggtgtcca gcccgaacat ctcgatcagt
    10321 cgtgcggcgt actccttgtc ccagtggggg tagtacagtg cggcggcgcg caggtattcc
    10381 ttcaccttca gtgtgttctg gccgaacagc agcatgggcg aaagctcgcg ggaaaagcag
    10441 atctcgtcca gtgctttctg gttttcccac acgcgctccc cgttgtaggt aacgaggccg
    10501 gaatcgtggg tattctgtgc cgtcagaatg ctgagcagcg tggttttgcc cgcgccgttg
    10561 cgtccgatga ggccatagat cttgcctggc tcgatggtca gcgagacgct gtgcagcacc
    10621 tcttttttgt tgtagctttt gaccacatcc gtggcctgca atactccgtt cgtcatgtcc
    10681 ctgtccctcc gttacgtttt gtccgccgcg gcctgttcaa tcatggccat cagctcttcc
    10741 ttgccgatgg caagggtgcg cgcctcgtcc acaaggctcc tcacatatgt ttcaaaaaaa
    10801 ctgtctttgc gcttttggat gattttctgt ttgctgcccg ccgcaacgaa catgccgatc
    10861 ccccgtttct tgtacaaaat gccctcgtct accaacaggt tcagcccttt ggccgccgtg
    10921 gccggattga tgctgaacgc ttttgcaagt tcgttggtgc tgggcgcctg ttcctcttcc
    10981 aaaagcaggt cccgcacgat gttgtcctcc agcatggctg cgatctgcag atagatggag
    11041 gtggaatcat tcaggtttcc attcaattgt ctgcgctcct tccctccaaa ggttatttgg
    11101 ttatgtggtt cattacttgt gtaactaact atacaacatg caaagacggt tgtcaatccc
    11161 tccgtcaaaa atttttgttt tccctgcggc agatttcccg ccaaagaaaa gcgccggagc
    11221 cttcgaaaag gctccggcgc aggcgggaac ttattgagcg gatggccccg catcggggtc
    11281 gaaccgtgtg agaaactggc agtacacata aaggttctgg acctttgttg cctcattgcc
    11341 gtactccgtg cggatactgg gctgcgtctc cgacggcgtg acataaaggc ggaacgcaat
    11401 ggcgtcctgg tccaccgcca tgttttccac gggtgcgtaa ttgaagttgc ggatggcaac
    11461 gtccagatgc ttgtacagca gctgttcgat ctcggcgttg gaaaagcacc ggacgacctc
    11521 acggctgccg tgcatttcaa tgatggcttt tacttcctcg aacgagatct cctgtaaacg
    11581 gaaacagccc tgatgcagca gggcgagcat ggatgcgttg tcaagcagca gcagcggttt
    11641 ggatacggcc atggtactct tcctttctgt ttgaaaagtg gaggaacagc cctccctgtc
    11701 tttatagtac cgtgcagaca tccaaattgt caattattgt taaatttata tcgttttata
    11761 agttgattgt tgtgaaatgt atcaaaaacg aactgccgca cttggtggga atgcggaatt
    11821 tacagccggg cgggatgctc ggccagccat tcctccagaa tttgagccgc cagcgccacc
    11881 agctccgggc aggggcgctt tttataatat tccggcgtgc gcgcctcggc ctgcggtgtg
    11941 ccctcgggac gggccaggcc cagcagctcc cggcagatga tgctgccgtt ttcggcccgg
    12001 aagcgcccgg ccagctcctg gatcatcgcg taggtatcgg ttttctccgg cccggctttg
    12061 gggtcgttgt agccgcgcag cgcgcttgcg gccagcgtca taccgcttac cgcaccgcat
    12121 acctcccgca tccgtcccat gcctccgcca aagccgctgg ccaggcgtgc cgcggcgtct
    12181 accgaaaggc ccatctccgg tgcgaacgcc ccggccacag cctgcgcgca gttatagccc
    12241 tccagaaaca gcgctttcgc gcgttgtgct ttttcactca tatccgatca gtcctttacc
    12301 agggaaaatg cagttacgtc gaacaggccg cggtccgtca ggcgcaattc cggtataacg
    12361 ggcagcgcca gaaaggaaag gctggtaaac gggtccacat ggtacgggat acccatggcc
    12421 tttgcccgtt ccagaatggc gcccgctccg gcctgtatct cctcccacgg ggcggtgctc
    12481 atcaggcctc ccacgcgcag gggcagcgcg cccagtattt ttccgccgcc ggccagcaca
    12541 tatccgccgc cgatttcttt caaatggttg atggccagcg cgaggtccgc gtcgttgtct
    12601 cccgccgcga tgacgttgtg gctgtcgtgc gccacgctgg tggcaatggc tccgcctgtg
    12661 atgccgtagc ccttcagcgg gcaaacggca acgcccccgt ttttgccgtg gcgttccacc
    12721 acgcatagtt tggtataaac ggcgttgggc cggaaaaagc cgttttccga aggaacctcc
    12781 tccactgcgt gccgcgtgac gatctggtac ggcaccatct caatgacatg ggccgggccc
    12841 gcgacgggca gcgcaaggtc ggcaggagcc acgttctcaa agtgtaccga atgcagcagc
    12901 gcatcgggta cgggggcggt ggcaaggcct gcaaagcgct cctccacggg ccggccgtcc
    12961 ttgtaaaccg cgcgcacggt catctgcttg aggctgtcca gcaggacaag gtcggcccga
    13021 tagcccgcgg ccacggcgcc caggtcccgc aggccgtatt cgcgggctgc gttccaggtg
    13081 gccattttga tggcggtgac aggcttcatg cccaggctga tggcctggcg gacattccat
    13141 cgcagatggc cgtcccgggc aatgtcgtcc aaatgcttgt cgtcggtgca gaacagaaaa
    13201 cggtcggcag gcaggtcgct gtccagcagg ccgtttacaa gcaccgaaag gtttttcgcc
    13261 gcgctgccct cgcgcaccag cacagccagg cccgcgcgca gcttttcggc ggcctctgca
    13321 aaggtggtgc actcatgctc ggtgcggatg cccgcggctg cataggcctg cagctctctg
    13381 ccagaaaggc cgggagcgtg gccgtcggcc cgctttcccg catccgcggc cagcgccagc
    13441 ttttccagta cggtttgccg cccggccagc acgtcgggat aacacatcac ctcgcccaga
    13501 cccagtacgc ggggatgcgc cataaacggg gccatatctg cggcggtgaa atccgcgccg
    13561 tttgtttcaa agggggtggc gggcacgctg gagggcagca tcaggtacac gttcagcggc
    13621 aggttttccg ttgcatccag cagatattgt acggcctgtg caccgcatac gttgacgatc
    13681 tcatgcgggt cggccacaat ggtggtggtg ccgctgcggg tggcggcccg ggcgaattca
    13741 aagggcgcgg caaggctgga ttcgatgtgg atgtgcgcgt cgatgaaacc cggacacagg
    13801 aatttgtccg tgcagtccag ttctgtccgt ccccggtagc ttcccacgcc cgcaatcacg
    13861 ccgcccgcca ccgccacgtc tgcagtgatg atttcctcgg aaaaaacatt gaccacgttg
    13921 acgtttttga acacgaggtc ggcgggggcg cgccccgccg ccacgtcgat gcatgctttg
    13981 tccgctttca ttgtccggcc gcctttcctg tttttggttt ttaccagtat actacattat
    14041 ataaaaaacg aaaagggctc cggcacattc gcgcaggcag gacgctctgc gcttccccgc
    14101 ccaaaaacaa aaagcccgct ttcagcgggc tttcggctct gcatcctgtc aaatttttgt
    14161 acgcagtggt atggccgcgt cagagcgcgg ccttgagccg ccgggccagc cgcatcagca
    14221 gtgtgggatt ccggcggtaa taatccgccc accaggcctt ttcggcgcgc ttgtcatagg
    14281 gaagacggct gtacggctgg gtgcggggat atgcgcgttc atactcgcag ttgagacgag
    14341 ggtccatttg catgaaaaca agcctccttt ttgaggccgc ccggcaccgg gcggcacagt
    14401 tgcgtgcaga gcgttttgcg ggatgtgtta ttctatttta tgcccccgac ggggacggtg
    14461 tgagcttccg ttcctttccg ctttcagtat atcatgtccg ccagtgaacg acaatatacc
    14521 gtttgcacga aagcgcggcc attgcttaca gcaacctgcc ggaacggttt ttccggcgaa
    14581 tacacctgtg tgccgcgcgc tctcatgctg cggtttttgc cgcagttgtg acgaaattgt
    14641 aaaagttgaa aaaattcgcg cattttgccc cgcagggctt gacatactaa aaaagtgttg
    14701 taaaataaat ttagcactcg aacagataga gtgctaaaca gcaagggagg aaaggtggtg
    14761 tgggcggtgc cgatggacga tcggaaacgg cgtgtgctgc gggcgatcgt aaccctgtac
    14821 ggcgcggacg gagagccggt tggcagcggt ctgctggccg aacagttcgg acatgcggtg
    14881 tccagcgcca cgctgcgcaa cgaaatggcc gcgctgacaa agctgggcct tctggagcag
    14941 ccccacacca gcgccgggcg cgtgcccagc gccaagggct accgttacta catcgacaat
    15001 cttctggata caggcgcccg ccttcccgct gaggaaaaac ggcagatcga ccttttgttc
    15061 cgcgagatgg actacgaccc cgaacgtctg gccgaaagcg ccgcaagggc tttggcggac
    15121 ctgacgggct actgtgtcgt ggctacaacg cccaaaagcg aggacctgtg catcgcccat
    15181 tatgaggtga tgcaggtggg ccgctacacg gcggcggtgc tggccgtcac caatgcgggc
    15241 ggtgtgcgca cgcgcacggc aaagctggat ttcgcgctgc ggcccggcga cgcccagcgg
    15301 ctgtcccaag cgctgaacac ctaccttacc ttccgcagcg cggccgacgt gcgcgaggaa
    15361 cagatgcgta ccgcagcaca gagcctgggc agtgcggtat attacccggt gctttcggcg
    15421 gcggtgacgc tgctgcggga agcgggacgg cccaatgtat atctggaggg ccagcagaac
    15481 ctgctgaatt atgccgagct tgcggacagc ttccgcacgc tgatggaatt cttcagcgac
    15541 acagaagccg tcaagcgcta cgtcagcccc aagagcgagc gcaccacggt gctgctgggc
    15601 gacgaactgc ccgagtaccg catgccgggc ttctgcatca tgacgaagcg ctatctcgcg
    15661 ggaggcgggc tgacgggggc catcggcatc gtaggtccaa cgcgcatgcc ctaccgggaa
    15721 acgatcccgc ggcttgaata tttcgcattg ctgctcggcg aatgcatgtc gggcagggtg
    15781 caggaggagt agcaacatgg cagacacgga aaaagacgtg aaccgcgcgg ccgccgcgcc
    15841 gcaggaggaa gcgtccgccg cgcaggcggc gcccgaggca ccgcagcctg aaacgcagga
    15901 tgcagaaaag gcggaaaaaa aggaaaagca cgcagaaggc aaggcgcatg ccgagctggc
    15961 cgcactgaaa accaagctgg agcaggctga aaaaaagctg gctgaaacga aagacacgct
    16021 gctgcgcacc gccgcggaat atgaaaactt ccgcaagcgc agcgccaggg agcacgatgc
    16081 cgcgttcgac aatggcgtga gcttcgcggt gaacacgctg ctgcccgtgc tggacacgct
    16141 ggagatcgcc gccgacgcgc ccaccgcgga cgagagctat aaaaagggcg tgaccatgac
    16201 gctggacaaa tgccgcgagg cgttcggcaa aatgggtgtc agcgagatcg aggcgcaggg
    16261 caagcccttc gaccccgagc tgcacgcagc cgtgctgcag cagccctcta ccgacgacat
    16321 gcccagcggc accgtcacgc aggtgatgca gaaaggttat ctgctccacg acaaggtcat
    16381 ccgccacgcg gccgtggctg tggccgaata ggctgacatc tcaaagccga aaggcttttt
    16441 gatataaatt cagaaatcac cacatatatt caccgagttt tgataaaaga ggagtctgag
    16501 agttatgagc aagattattg gtatcgacct tggcaccaca aacagctgtg ttgccgtcat
    16561 cgagggcggc gagcccgtcg tcatcgccaa cgccgagggc gcgcgtacca cgccgtccgt
    16621 cgtgggcttc accaaaacag gcgagcgcct ggtgggccag gtggcaaagc gccaggccat
    16681 caccaacccc gaccgtacca tcgcttccat caagcgccat atgggcacgg actataaagt
    16741 ggacatcgac ggcaagaaat acaccccgca ggagatcagc gccatgattt tggccaagct
    16801 gaaagcggac gccgagagct atctgggcga aaaggtcacc gaggcggtca tcaccgtacc
    16861 ggcgtacttc aacgacagcc agcgccaggc caccaaggat gccggcgcca tcgccggcct
    16921 ggaggtcaag cgcatcatca acgagccgac ggccgcggct cttgcctacg gcgcggacaa
    16981 agaagacgac cagaagatca tggtctacga cctgggcggc ggtacgttcg acgtctccat
    17041 catcgagatg ggcgacggcg tcaccgaagt gcttgccact gcgggcgata cgcatctggg
    17101 cggcgatgac tttgacaagc gtatcatcga ctggctggcc gatgagttcc agaaagagaa
    17161 cggcgtggac ctgcgcaagg acaaaatggc cgcgcagcgc ctgaaggagg ccgccgaaaa
    17221 ggccaagatc gagctgtccg gcgtggccag cacggccatc aacctgccgt tcatcacggc
    17281 ggacgccaac ggcccgaagc atcttgatac cacgctgaca cgcgcaaaat tcaacgagct
    17341 gaccgcggac ctggtggagc gtacgatggg cccgacgcgc caggcgctga aggacgcggg
    17401 cctgcagccc ggcgacctgc ataaggtgct gctggtgggc ggctccaccc gtatcccggc
    17461 cgtgcaggag gccgtgaaga acttcatgaa gaccgagccc agcaagggca tcaacccgga
    17521 cgagtgcgtc gccatcggcg cggccatcca gggcggcgtg ctgggcggcg acgtcaaggg
    17581 cctgctgctg ctggacgtta caccgctttc cctgggcatc gagaccatgg gcggcgtctg
    17641 cacc
//
LOCUS       DAWBSB010000016        17642 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_846_length_17642_cov_3.682720, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000016 DAWBSB010000000
VERSION     DAWBSB010000016.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 17642)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 17642)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17642
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_846_length_17642_cov_3.682720"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3199 a         5198 c         5712 g         3533 t
ORIGIN      
        1 atgatgtgat ggtggtgcac ggcaacggcg cgtccatcgg cgtgctgaaa gaggcgggtg
       61 tggaagacgc gcatctgctt attgccgcca ccagccggga cgagacgaac ctgctcacct
      121 gcatcgtggc aaaaaagctg ggcgcaaagc acaccattgc ccgggtgcgc aaccccgaat
      181 atgccgagca gctggtgctg atgcgggacg agctgggcct ttccatgacc atcaatccgg
      241 agcttgcggc cgcgcgggag atccatcacc tgctgcagtt cccctcattt ctcaagcggg
      301 attcctttgc caagggccgt gtggagatcg tagagatcca tgtgggcgag gattccaagc
      361 tggcgggcat cccgctcaac cgcctgtatg agatcgcgcg ggtgcgcgtg cttgtgtgcg
      421 tggtggagcg cggcggcacc gtgcatatcc cggacggcag cttcacgctg caggcggggg
      481 acaatatcta tgtcactgcc gattctcagg accttgccca gctcatcaag catcttggca
      541 ttgtcaagca gaaggtgcgc aacgccatca tcgtgggtgg cagccgcatc gcctattatc
      601 tggccatgcg ctgcctgcac gcggggctgg gcgtcaagat catcgagcag aaccatgagc
      661 gctgtgtgga gctggctgag ctgcttccca gcgccgtcat catcgaagcc gacggctccc
      721 ggcaggacat tctggctgca gagggcatca gctccacgga cgcggtcatc acattgacga
      781 atatggatga ggagaatctc atcatttcca tgtatgccag ccacatcggg gtgccaaaag
      841 tcatcacaaa ggtgaaccgc accgaatatg tggaagcctt caagagcatg ggcatcgaca
      901 cgttcatcag ccccaaggcg ctgtgcagca cggacatcgt gcgctatgtg cgcgccatgc
      961 agaacaccac gggcggcagc gttattacgc tgcaccgcat ggtggagggc aaggccgagg
     1021 cgctggaatt ccgcgcatcc gagaccacgc gccacctggg cgagacactt ttggacattc
     1081 agcttaagcc cgagatattg atcgcctgca tcacccacaa gggacgcacc atcatcccgc
     1141 ggggcagcga cgtgttccag gccggggaca ccgtcatcgt cgtcacgacg agcggccagc
     1201 ccatcaacga cctgaatgac atttttgccg actgagggcc ggcattcccg gaggaacgaa
     1261 tgaacaagaa aatgatcggc aagctggttg gcgtgctctt tttcctggag gcgcttttca
     1321 tgctgccagc cattgccgtg tgctttgttt acggcgagac gggcgcaccc ctgcggggct
     1381 ttttgtggac ggtggcgctg ctgctggcgc tgtccggcgc gctgtacggc gtgtgtgcga
     1441 aggccacgcg ggagttttac gccaaagagg gcttcatcat cgtggccctg ggctggatcc
     1501 tgctctcggc catgggcgcg ctgccgtttt acatctcggg cgagataccg cattatgtag
     1561 acgcgttttt tgagacggtg tccggtttta ccaccacagg cgcgtctgtt ctgacagacg
     1621 tggaggccat gagccacggg ctgctgtact ggcgcagctt tacacactgg ctgggcggta
     1681 tgggcatgct ggtattcctg ctggccgtgg tgcccacggg aaagggagcg ggttacagca
     1741 tccatctgct gcgggccgaa agccccggcc ccagcgtggg taaaatggtg ccccgcatgc
     1801 gccagacggc cagcctgctg tataagatat acatcgtgct caccgtcctg tgcgtattgt
     1861 ttctgctggc gggcggcatg cccctgttcg acagcctgtg cacggccttc ggtacggcgg
     1921 gcacgggcgg cttcggcatc aaaaacaaca gcatggcgga ttacagcccg tacctgcaga
     1981 atgtgtgtac ggtgttcatg gcgctgttcg gcgtaaactt cagcgtctat tttctgcttt
     2041 tgctgcggga gtggcgcggg gcgctgctgg acgaagagct gcttttgtac ggcggtatca
     2101 tgcttacggc catgtttgtc attgcggcgg acatcacgcc gcttttctcc ggcttcggcg
     2161 aggcgttcca tcatgcgtcc ttcaccgtca gcagcgtgat gacgaccacg ggcttcgcca
     2221 ccgaagattt caacctgtgg ccgcagctct cccgggccat cctgtgcatt ctgatggtgg
     2281 tgggcgcgtc cgcgggcagc acgggcggcg gcatcaaggt agcgcgcctg cttctgctgt
     2341 tcaagagcct tgcggctgag gtgcgccgca tgctgcgccc gcgcagtgtg aatgtggtgc
     2401 gcgtgaacgg ccgcgttgtg aaggacgagg tgctgcgcgg tgtgaacgtc tatatggcag
     2461 cctactgcgc catcgcggtg gccagctttc tggccgtctc cgtggatgat ttttctctgg
     2521 aaacgaacct gacggccgtg ctggcatgct tcaacaacat cggcccgggg ctggacctcg
     2581 tggggccggc cagcaactat tccctgtttt caactttttc caaggttgtg ctttcgttgg
     2641 atatgcttct gggcagactg gaaatattcc ccattattgt gctgttcagc cgccatacct
     2701 gggaccgcac gcgctgaaaa ccggatacgg acgcctctgg gcgcgcccgg cacaggctgc
     2761 cgcggcgcgt ctgtttgcat ttttgcacat tttttgttat agtatttttg tttgcttccg
     2821 gccgcacggc gggaagggac cctattgatt tcaggagagt ggttttttgg atacccccgg
     2881 ccctggtatg tacctggcac tgctgctttt gctggcgttc tcggcgttct tctcggcaag
     2941 cgagacggcg ctttcgtcgg tcaaccgcat ccgcatgaag aataaggcgg atgacggaga
     3001 caaaaaggcg cagcgcgtgc tgggcctttc cgatgattac gacaggacgc tgtccgccat
     3061 tctcatcggc aacaatgtgg taaaccttac tgcatcgtcg atctccaccg tcattgccac
     3121 gtcgctgctg ggacagcagg gcgcggcagt ggccacggcc gttatcacgg tgctggtgct
     3181 cgtctttggc gagatattgc ccaagagctt cgccaaggac catgcggagc gcgtgagcat
     3241 ggccatgggc ggcccgcttt acatcgtcaa aacgctgctg gctccgctgg tgtgggtctt
     3301 tgtgcagatc aagcgcctgt ttacaggccg gcgctccagt gagctgaacg tgcagcctag
     3361 cgtgactgag gaggaactca aaaccatcat cgacaccgtc ggtgaagaag gcgtgctgga
     3421 ccgccaggaa acagacatca tccagtcggc catagagttc gacaatacga cggtgcagga
     3481 cattctggtg ccccgggtgg atatggccgc ggcggcgctg gacgcgccgc cggaggagat
     3541 tatccggctg tgtgtggagg gcggttattc gcgcatcccg gtgtatgaag ggaccattga
     3601 caacgtggtg ggcgtactgt atgcgaagga cctgctggcc cggctggccg cgggaaagcc
     3661 catcgagccc gcggcgctca aacgggacgt gctttttgtt taccgcagca agcgcatcag
     3721 cgagctgctg gcggagttcc gccgggcaaa gcagcacatg gccgttgtga cggatgaaca
     3781 cggcggcacg ctggggctcg tgaccatgga ggatatcctg gaggagcttg tgggcgagat
     3841 ctgggacgag acggacaagg acgaaacgcc catccggcag ctgagcgaga acagctggca
     3901 ggtggagggc gacgtgaacg tggaggactt tttcgacgcc atcggcttcg tggacaaaac
     3961 gttcgactgc gactacaact ctgtgggcgg ttgggcgctg gccgagctgg agcatattcc
     4021 cagcgccggg gaaagctttg gctacaagca gcttcatgtg accatccgtt cagtgaagga
     4081 ccagcgcatc gagagcctgc tggtgacgcg cggcgagcac ggcggcgggg acgctccgcc
     4141 ggaatagctc ccgggagatg aaaaagggag agaagtaaaa gaagcgtccg gggaaaaagc
     4201 tggcggtggc aaagccatgc atctttgcgc agcaaagtgc aagccacgcg gcgaatgccg
     4261 ccaaaaaggg gtggaggacc cccttttcat ttaaaataac cccgcggcgc cccgcagggc
     4321 cagtgccgag gcgtgtagag cgaagcggca gccgaaggcc gccgtgcgcg gcgaacgggg
     4381 ccgcaggccc tgttcgcagg cagcttggcg caaaagcgca gctttttcga caagctggaa
     4441 caccgccgcc ccgcagccga agctgccggg gcggcggtgt ttgtttttgc gcgcggttca
     4501 gcccggcggc acgcattcca gcacgctgcg tgcaatggcg tcccctgcgg cccagagctg
     4561 cggctcgctg gtgacgtttt cgtattcgga ggggttcacc ataaagcccg cctccagcag
     4621 aactgcggga cacacggtgc tgcgtgtgac ataatagtag ccccactgag cgccccggtc
     4681 cggccgggag gtggcctgcg ttacattgcg caccagcgcc tgggccagcg ctttgcccgc
     4741 ggggtagaag taatagcact cggtgcccgt ctgcaggttc gcatctacgg aaaggtcgat
     4801 ggaattgtgg tggacggcga tgaaaaaatc aggctggccg gcaacaatgg ctctgttgcg
     4861 ctcctccaga gagaggaagg aatcgtctgt ccggatggtc tgcaccgtgg cgccaagctg
     4921 ctccaaacgg tattttgcgg ccagcgtcag agccagattg acgtcctttt ccgcgggcgc
     4981 cccggcgccc gccgcgccca ttgcgccggg gtcatggtca ccgtggcctg cgtccagcag
     5041 cacggccact cccgtcagag gcttgccgaa tacatcgctg cgcaccggct gctttttcag
     5101 gatgacctgc gtggtgccgt ccgcatattc cacggtatgc ccccagacgg gctgggtaaa
     5161 ggtaagcgtg acgcgtgtgc agccatcgcc cggatccacc gcggcgctct gcaccaaagc
     5221 ggaaccggaa acggcgaaat ccgccgcgaa gtccgcatcg taaaaatcca tcaccagcgc
     5281 gccgtccacc atgttggtat aggccagcgg tgtgccgctg cccgcaaagg tgagcacctc
     5341 gcccgtatcc gtcgccgcgg cggccgcgcc tgtaaatgag gcgcggggcg tttccggcgg
     5401 cagcggctct gcattgtaag ccagcacaaa atccccgctg gcaagctgat aggcccaggt
     5461 gcgcttgccg ccgcgcagcg tttcggtgca ggcggccacc gtggcaactg cgccctggcg
     5521 tacggtctcg ctgatgtttc cgtcggacgc ggggtcatac agcaggctgg cgatccagtt
     5581 tcggatgcgc acaggcgtgc ccggctccac ttccgcagtg tcgtcgtggg ggacctccgt
     5641 tgtgccgccg ctcccgccgc ctgcgggcgc gggccggttc acggtcacag agacgctggt
     5701 tccgccctgt gtgagggtat acacattgct gccctctgcc acgtccacca gcacgccgaa
     5761 ggttccgccc gggccgcggc tctccacggg tgtgccgttc agcagcagct cctgcgccgg
     5821 gtctgacgtg cccatcacaa agcaggctga ggtgtagacg gccgcaccgt ccgccggata
     5881 ggataccgcc agtgtcccgc cgggcgtata ctccagcagc gcaggccgcg ccgcactgcc
     5941 gtccagcgcg ctggccagca cgctgaaggc ctccggctgc gcccggcacc gcgcataatc
     6001 gaatacggcg ccgtggaagc cgtctgtgtc cagcgaagca aggaacaggg acgacggatc
     6061 gttgaaggcg gcctcgtccg cgtagaatac cgcctgccgg gaaatactgt cgaacagggc
     6121 gctgccttcg acatacaggc ctgcaacggc atattttttc tgcatacgcg ccagcgctgc
     6181 ctcccaatcc gcatttccgg agagctcggg cgtcacagcg tacacggcca gccccgcctg
     6241 ggacgcctgc tcacacagtg cgcgcagcgg gtcgtatttg tgaaacaggc tgtcgtctgc
     6301 cgccgcgccg ggccaggtct cataagcgcg gtcgcggaaa gcgatgctgg acaattcggc
     6361 gtccgcaacg tccagaaaca gcgcgttcag cccatgggcc tgggcatagg ccgccgcgtc
     6421 tgcgaacagc gtatccagaa cctccggccc gccggaggag agcccgcccg cagccgttgg
     6481 atagctccac gagagccgca gtccccgcag cgtgccctgg ggcgccgccg cactgggcat
     6541 atcccggcgc atggcacggg gccacagcag ccacatgccg gttcccagcg cggccagcag
     6601 caccgtagcc agaacaaaaa gcaccacgcg gcccgcacgg cgccgcctgc gtttgaaatg
     6661 catccttgaa aacccccttt tttccgcagt tcacggcaaa ataaaacaaa atactgcctt
     6721 tactatacca tagcataccc attttgtgta tccaaatcgt atcccgtgca gacagccgga
     6781 gccgcgtttt tcgccctcct gtgaaaaaac ggctaccaaa cacgcccccc atatgttatg
     6841 ctatgggagt agatttgatt ttatggcact ttaaaggaga cgatattttg cagatacccg
     6901 ataccgcatt tacccacggc ggaaaatttc acgccgacga tgtgttcagc gcggcgcttt
     6961 tgaagatatg caaccccaac attgccatac agcgtggctt tgcggtgcct caaggctttg
     7021 cagggctggt attcgacatt ggggacggcc cgttcgatca tcatgccaaa aacagccctg
     7081 tgcgggagaa cggtgtgccc tatgcggctt tcggcctgct gtggcgggag ctggggccgc
     7141 agcttgtcgg ccccgtggac gcgggccgct ttgacgagag ctttgtacag ccgctggatc
     7201 tggacgataa tacgggctgc ggcaaccagc tggccaatat catcgccgcg tacaatcccc
     7261 ggtgggatgg cgcggaccgt ccggacgact gctttgcaca ggccgtggcg ctggcgcagg
     7321 atatgctggc tcacaagctg gaggccatcc gcagcgtgca gcgcgccgcc gccgaggtga
     7381 acgaggcgct gggccgcatg aagcggcgca tcgtgcggct ctcccgcttc gcgccgtgga
     7441 agcagcagct cattcccagc aaggcgcgtt ttgtggtata tccgtcccag cggggcggct
     7501 gggcggcgca ggctgtgccc gcgtcctttg gctcgcccgc gctgaaggtt ccgttccccg
     7561 cgcactgggc gggtgcgtcg gagcaggagc tgcccggtct ttcgggcatc gagaccctgc
     7621 ggttctgcca cgcggggcgg ttccttgtca ctgcgggcac ggaggaggac gctgtggccg
     7681 cgtgtgaggc cgcgatggag ctgaacggat aatggattgg gtgtacatgc tggagtgcgg
     7741 cgacggcagc ctgtacacag gctggacgaa cgatctggcc aggcgccttg ccgcccacca
     7801 aagcgggcgc ggcgcgaagt ataccagggg acgcgcgccc gtgcgtctgg tatatgcgga
     7861 gcagtgtacg gacaaaagcg ccgctctgcg gcgggaagcc gctgtcaagg cactgccgcg
     7921 ggcgcgcaag ctggagctag cgcggcaatg ggaaacggag gaaaaagcaa tggctgttgc
     7981 gatggattca caggaagcgc gcaggcgtat ggaggaaggc cggctttatc tgccggggga
     8041 tgaggccatc atggccgaac agatggactg tctggagaag cagtatgatt acaacgccac
     8101 gcgccctcac gagcaggaaa ggcgcgccgc cctgctgcgg gaaatgttcg cccagatcgg
     8161 tgaaaactgt tatatcgagc cgccgctgca cgccaactgg ggcggccgcc atgtgcattt
     8221 cggcagcggg gtgtacgcaa acttcaacct gacgctggtg gacgacgccc atatttatgt
     8281 gggcgactgt gtgatgttcg gccccaatgt cacggtggcc acggcggggc atcccattga
     8341 gccgggcctg cgccgtcagg ccatgcagta caacgccgac gtccgcatcg gcagcaacgt
     8401 gtgggtgggc gcgggcgcgg tcatcctgcc gggcgtgacc attggggatg acacggtcat
     8461 cggcgcgggc agcgtggtga cgaaggacat cccggcgggc gtggtagccg tgggctgccc
     8521 ctgccgtgtg ctgcgcccca tcggcccgca ggacagggag acctacttcc ggggccggaa
     8581 gatcgatgtg ccgctggaat agcagtgcgc ggcatccggc acagaaggga aagcatgtca
     8641 aagtggaaaa ggaatatctg atccggcagg ccccggcaaa ggcgctgctc gcgttttcaa
     8701 cgcctatgat catcggcaat ctgttccagc agctttacac gatgacggat tctgttattg
     8761 tgggccaggc tgtgggtgaa agcgcgttgg cggccgtggg ggcttcctat gctctgacga
     8821 atgtgttcat ctccatcgcc atcggcggcg gggtgggcgc gtccgttctc accagccaag
     8881 ccttcgggcg gcgggactat cacaggatga agcgctttat ctccacggcg ctgttcacat
     8941 ttctggccgt gagcctgctg ctgggcgggt tcgggcttgc cttcagcggg cagatcatgg
     9001 catggctgcg tacgcccgcg tccattctgg acgatgcggc ggtctatctc aatatttatt
     9061 ttctgggcct gccctttttg tttatgtaca atgtgctctc agccatgttt aacgcgctgg
     9121 ggcgttccaa ggtgccgctg tacctgttga ttttttcttc ggtgctgaat gtggggctgg
     9181 acctgtatct ggtgctgacg ctgaggatgg gcgtggcggg cgtcgcatgg gcaacgctca
     9241 ttgcccaggg agtatccgcc gtggctgctt tcctgttgtt tgtgcgggag ctgcgggcct
     9301 acgatgcccc gtcggccgtg tgcttcgatg tgcgggaact gaaaaacatg gcccgcgtgg
     9361 cgctgccgtc cgtctttcag caatccacgg tgtccatcgg catgatgctg gtacagtccg
     9421 tggtgaacag cttcggcgcc gagacgctgg cgggttattc cgcggccatg cgtatcgaga
     9481 gcatctgcac cgtgcccatg gcggcggtgg gcaacgcgat gtcctcctac accgcgcaga
     9541 acatcggggc gggggagtac agccgtgtgc gcaggggata ccgggcgtgc tatggcattt
     9601 tggccgcgtt cgcgctggtg atcttcgcgg cgcttcagtg cttttcgcag cagctcattg
     9661 cgctgttcct caacgcggaa agctcacgcc tcgcgctgga gaccggaacg tcctgcattc
     9721 gtttcctggg gtggttctat atcctcatcg gcctgaaaat ggccacggac ggcgtgctgc
     9781 gcggcgcggg ggacatgggc gcgtttaccg cagcaaatct tgtcaacctg acgctgcgcg
     9841 tggttctggc ggcagtgctg gcgccccggc tgggcatccg gatggtatgg tatgcggtgc
     9901 ctgtgggctg ggcggccaat taccttatct cgtttttgcg ttaccgaacg ggccgctggc
     9961 gggagcgcgg cgcagggctg ctggcctgac cgcggcagaa aaatttgctt gacagccgcg
    10021 aaaacttgtg tataatatgg aatatacgca aaaaacgatg acgagaagag taagctgcgc
    10081 aggctttttc agagagttcc cggtcggtgg aagggagcaa agcgggcata gctgaacatg
    10141 gcctctgagt tgcgtgcgcg agcccttggc aagcgcatga cgggcgcacc cgttacagtg
    10201 ccgcgctgta acccgtttca tccgcgggcg cagcggctga ggctttttcc gtgaggaaag
    10261 agcgaaacaa ggtggtaccg cgagcatttc tgcccgtcct tggaacgtaa gttccgggga
    10321 cgggcttttt gtttgctccg ccgcagagcg gtgcacaggg gaaaagaaaa ggagaatcgg
    10381 ttttgatcac gatatcgcat ctgacgaaga tctttgaggg gaagggcccg cgcgtgacgg
    10441 cgctggagga tgtgaacctg gagatcggca aaggagacat ctacggcatc atcggcatgt
    10501 cgggcgcggg aaaatccacg ctgctgcgct gtttgacgct gctggagcgc cccacctccg
    10561 ggcagatcat gctcgccggg caggacatcg cttccctttc cggcgcgcag ctgcgcgcgg
    10621 cgcgccgggg catgggcgtg gtgttccagg gttacaatct gctgatgcag aagaccgtgg
    10681 ccgaaaacgt tgccttcccc ctccggctgg agaagggata cgacaaggcg gcggtgcagg
    10741 cccgggtggc ggagcttttg gccattgtcg gcctggagga ccgcgcgggc gcgtacccgg
    10801 cccagctttc cggcgggcag aagcagcgcg tggccatcgc ccgcgcgctg gccacacagc
    10861 ccgagatact gctgtgtgat gagcccacca gtgcgttgga cccgcttacg acgaagtcca
    10921 tgctggccct gctgacggat atcaacaaaa agctgggcgt caccatcatc atcatcaccc
    10981 acgagctttc ggtggtacag gccatctgtt cccatgtggc cgtcatcagt gaggggcgtc
    11041 tggcagagtc tggcgcggtg gcggatgtgt tcggcagccc gcgcagcgct gtgacaaagc
    11101 tgctgctggg cagagaggag taaatacaat ggcggagttt ttcaacgaat acggcgcgct
    11161 gctgtgggag ggcacgctgg aaaccttata tatgacgctg tttgccacgc tgtttgcata
    11221 tgtgctgggc ctgcccatgg gcgtgctgct cacagtgacg aaggcgggcg gggtggcccc
    11281 tgcgccgcgt ttcaacgcgg tgttcggatg gctggtgaac cttctgcgca gcctgccgtt
    11341 catcattttg atgtttttca tcattccgtt tacacggctg ctggtgggta cgtccatcgg
    11401 cgccacggca gcgctggtgc cgctcacgct ttcggccgcc ccgttcattg cgcgcatggt
    11461 ggagcagagc ctggaggaaa tcgacacagg cgtcatcgaa gctgcccagt gcatgggcgc
    11521 caccaggtgg cagatcgtca cgcgggtgct gctggtggaa agcgtgccca gcctgctgcg
    11581 ggggctgtcc atcagcctta tcaccatttt gggctatacg gccatcaccg gcagcgtggg
    11641 cgcggggggc ctgggcaacc tggcgttccg cttcggctat cagcggtacc agaaacccgt
    11701 catgtacgcc accgtgctgc tgctgatcct gctggtgtgc gtcatccaga tcatttttga
    11761 tcttgccgcc cgcaaagcgg acaagcgcac gcgctgaaca gggcgcctcc ggcttcggca
    11821 aacacgccgt ttgcggcggt ttgagatata ttggaaaaac agttgaggga aggaagtttg
    11881 ttatgaaaaa atttttggca ttggtattgg cgctggtact ggcgctgagc ctggcggcct
    11941 gctccggcag cgctggctat cagatcggca tcccggcgga cgccaccaat ggcggccgcg
    12001 ccctgctcct gctgcaggac ctgggcatcc ttacgctgaa ggagggcgtg ggcctggagg
    12061 caaccgaaca ggacatcgtt gaaaatccgc acaatgtcac catcaaggcg atggaggcgg
    12121 caaacctgcc cgccagcctg ccggacttgg atttcgccgt catcaacggc aactacgcct
    12181 ccggcgcggg cattggggat aaggtgctca cgacagagga cgcggaatcc gtggcggcgc
    12241 agacctacgg caatgtcgtt gcggtgaagg aaggccggga aaacgacccc gccgtgcagg
    12301 ctctggtggc cgtgctgatg agcgggaacg tgcaggcatg gatcgaggag agctacaacg
    12361 gcgttgtgat gcccatgggc gcacaggagc tggatattcc cgagattgcg gagcctgtga
    12421 cgctgaaggt gggcgcgtcg cccagccctc acgcggaaat cctggagcat gtgaagcccc
    12481 tcctggccga gcacaatgtg gagctagaca ttgtggaatt tgacgattac gtcatgccca
    12541 acaccggtgt ggaagacggc tcgctggacg ccaactactt ccagcaccag ccgtatctgg
    12601 acgatttcaa tgccgagcag agcacgcata tcgtcagcgt ggccaccgtg cactacgagc
    12661 cgatgggcat ttatccgggc aagaccgcaa gcctggacgt attcggcaaa taagttcttt
    12721 tcgcgcgcac cttttgggtg cgcgcatttt ttgcgcgaaa gtgcgcggtg ttttccgcaa
    12781 gcttcgcaat caaaaaacac tgcagacagc tgtttgcgcg cttgcaattt ttgcgcgccg
    12841 ggtgcaaaat agaggacgtg ggtttacacg ggctttacaa tcaccgcccg cggcccgagg
    12901 gaaagaaagc atggccgaaa aaggcctgcg gctgttttaa atgaagcgga catccggcgg
    12961 tctctccggg gacggcggcg ctgcccgcgg cgcacaaaaa ggggaaacta tggcacaaaa
    13021 aacattcgcg cgcccgctga tgtttacgcg tcaggcgctt tggaggctga tcctgccttt
    13081 ggtgctggag cagcttttgc ttgtgacggt gggtatggcc gatacggtga tggtctccac
    13141 ggtgggcgag gcggcggtct cgggcatttc tcttgtggac caggtgaacg tcctgctcat
    13201 tcagatattt gccgcgctgg ccacgggcgg cgccgtggtg gcaagccagt atctgggccg
    13261 gcgggacagg gaaaatgcct gccgctccgc caagcagctg gtatacgcca ccttcggcat
    13321 ggcggttgcc atcggcgcgc tggtgctggt gctcaaccgg cacatccttc gactggtgtt
    13381 cggcaatgtg gagccggacg taatgcaggc cgccgagaca tatttctggc tttcggcgct
    13441 gtcatacccg atgctggcgc tgtacaacgc gggggcggcg ctgttccgca gcatgggcaa
    13501 cagccgcatt tcgctgttcg catctcttat catgaacgtt atcaacatcg gcggcaacgc
    13561 gctgctcatc tatggcctga actggggcgt ggcgggcgcg gccacagcca cgctggcatc
    13621 ccgcacagtg gcgggcctga tgatgatgct gctgctgcgc aaccgggaca accccatctt
    13681 tctggagcgc ctgttccacc cggaatggaa cggcggcatc ctcaaaagca tcctgcgcgt
    13741 gggcgtgcca aacgggctgg aaaacggcat gttccagatc ggtaagctac tggtggcggg
    13801 gctcatcacc accttcggca cgtcggctat cgcggccaat gccatctgca acaatgtcgg
    13861 ttctatgtcc aacattcccg gctcggccat cggcctggcg atgattactg tggtgggtca
    13921 gtgcgtgggc gcaaaagact atcagcaggc gcggcattat acgaaaaccc tgttggcggc
    13981 ggcctatgtg agcatgggcc tgctgaatat cgcgctgttc ctgctggcgc cgtacctggt
    14041 gggcttttac gccatgccgc agacgaccac ggaccttgcg ctttctgtgc tgcaggtgaa
    14101 ctgcgttgtg acggtgctta tctggccgtc ctcgttcaca ctgcccaacg cgctgcgcgc
    14161 ggcgggcgac gccaggttca cgatggtgac gagcatggtg tccatgtggg tgttccgcat
    14221 cggcatgtcc tatgtgctgg gcgcgtggct gggcatgggc ttgttcggcg tctggacggc
    14281 catgcagatc gactgggccg tgcgttcact ggtgttcggc gtgcgttttc tgggccataa
    14341 atgggaaaag gcgcgggtcc tcgaaggatg agtgccccgc ggccggcatt gtgaaaataa
    14401 gctcccgcct taccgttttc aagatggtaa ggcgggagct ttctgctttt gagcggcaga
    14461 ccggtgtgcg gggccgttta tacgcccagc agcgcgcggg cgttctgttc tgttatacgc
    14521 agcacttcct ccaaaggcac ctgcttgatg tccgccactt tctgggcggt gaagcggatc
    14581 atgccggaat ggcattcttt tccccggaac ggttccggcg ccatgtaggg gcagtccgtt
    14641 tccagcacga tattttccag gctgagcgca gccaccgcct ccacggcgcg gcgcgcattt
    14701 ttaaatgtaa gcgctcctcc gaagcccagc agcatgccct gccgggcgat ccacagcgcg
    14761 tcgtcggcgc tgccggaaaa tgcgtgcaga atgcccctgg gccggtacct tttcaaaagc
    14821 gcgtaggttt cggcatgggc ttcgcggtca tgcacaagaa tgggcaggct gtgctcctgt
    14881 gctgcggcga gctctgcttc aaacagcgcc agctgggcgt cctccggcac gggccaatgg
    14941 tgatccagcc cgcattctcc aaccgccacg acccggggat cgccgtacaa cggttcaatg
    15001 gccgccagct cggcgcgcca atcgccatgg aattcatata ccgtggaggc gtcgtcctcg
    15061 atgaggctct ccggatggac gccggcagcg gcccagaccc agggccgcgt ttcagccagc
    15121 gccagagcgg cgcggctggt ggcaaggtct actccgcatt ccaccacgcc gcatacgccc
    15181 tgtgcgggca gactgtccag cacttcgtcc cggtgcccgt cgaaccggtg gctcgtgtaa
    15241 tgtgcatggg tatcgaatat catggtatcc ctccgttcaa aaacgccccg gaagcctccg
    15301 gggcgcatgt gtattctgaa acccatttaa agtttctttg cggactttct tttcaaagaa
    15361 agttaatgga tgcgcatgcc cgtgggtacg tcgtcgggga agaacaccac ggacgcaccg
    15421 ccgtcggcag tgtctgcggc catcaccatt ccctcggaaa cgtatttgcc gcccatcatg
    15481 gggcgcggcg caaggttggc cacgatgccc actttttttc cggccagatc ctccggcttg
    15541 taccatttgg cgatgccgga aaggatggtg cgctcacgtt cgccgtcgaa caccgtcagc
    15601 ttgagtagct ttttgctttc tttcatgttt tcgcaggccg tcacctggcc caccagcatt
    15661 tccacacggc agaatgtttc aaaatccacc tccggctcgt gccggatggg ctcggccgca
    15721 ggctccggtt ttgcgggagc cggagcgggc gcggcggcag ccttcgcggc ctcctgggcg
    15781 gcggccagct ccgccagttc cttcgcggcg tcgatgcggg ggaacagcgc ctcgcccttg
    15841 tgcacggtaa acggctcggg caggccgccg aaccggccca gcgtctcaaa gcgcatgctc
    15901 tcggccgtca ggccaagctg ctgcgccatt ttgggcgtgg tgttgggcaa aaacggcgta
    15961 agcagcacgg ctgccacacg cagtacttcg cacagattgt acagcactgc ggcaaggcgg
    16021 ggcaggttgg cctcgtcctt cgccagcacc cagggcgccg tttcgtcaat gtatttgttg
    16081 gcacgctgga tgaccctgaa aatctccgcc agcgcgttcg gcacctgcag aacgtccatg
    16141 catgccgtta ccttcgcggg cagggcctcc accatggcgg ccagctcgtc atccagcggc
    16201 tcggctttgc gctgggcggg cagagtgccg ccgaaatatt tttcgatcat cgccacactg
    16261 cgggagagca ggttgcccag gtcattggcg aggtcggcgt tgatacggtt gataagcgcc
    16321 tcattggaga agataccgtc gttgccgaac gggatctcac gcaggaggaa atagcgcagc
    16381 gcgtccacgc cgtagcggtc gcacaggacc acggggtcca ccacattgcc cttggatttg
    16441 ctcatcttgc cgccgtccag cagcacccag ccgtggccga acacgcgctt gggcagggga
    16501 agatccagcg ccatcagcat ggcgggccac aggatggtgt ggaagcgcag gatctcctta
    16561 cccacgatgt gcaggtccgc gggccagaat ttgtcgtaat cgtgatatgt ttcattgccg
    16621 tagcccagag ccgtgatgta gttggaaagc gcgtccaccc agacgtacat ggtgtgcttg
    16681 gggtcgaacg ggacgggaat gccccacttc acgctggtgc gggaaacgca ggtgtcctgc
    16741 aggccctggt tgatgaaagc gatcatctcg tttttgcgcg tttcaggctg aatgaaatcc
    16801 ggatgctctt catacagctt cagcaggcgg tccgcatatt tcccggtttt gaagaaatag
    16861 gcttcttcct cggcctcgta cacggggccg ccgcagtccg ggcatttgcc gtccacaagc
    16921 tggctgtctg tccagaagct ttcgcaaggc ttgcagtaat gccctttgta aacgcctttg
    16981 tagatatcgc cctgctcgta aagccgggtg aaaatttttt ggcagctctc cacatggtaa
    17041 tcgtcggtgg tgcggatgaa tcggtcgttg gaaatgtcca tcagtttcca cagctctttg
    17101 atgccggcga cgatctcatc cacatattcc ttcggcgtca cacctttggc cgcggccttg
    17161 tcctcaattt tctgcccgtg ctcatcggtg cccgtcagat acatcacatc atagccctgc
    17221 atgcgcttat accgcgcgat ggcgtcgcag atgacggtgg tgtagctgtg gccgatgtgc
    17281 agcttgtcgc tggggtagta gatcggtgtg gtgatgtaga aggtcttttt attttccata
    17341 ggtaccagtc cctttcgggg cgcgcgaatg aaacgcgccc tttttatttt gggccgcgcg
    17401 ggcggccgga aaaacggaag atgccgcggc gcttaaacgc ccggcgtttc ggcggcgcag
    17461 gcgtccgccg tatttcgcct tttgcgggag acacgggccg ccagcgcccg aggccgcata
    17521 ccggccgcgc gcctgcctgc tcggcgcatc cggatggttc cgccgcccgg catgacacaa
    17581 agcggcagcc gctgcaaagc ccgccgggca gatgcccggc cgcgtgcggc aaaatatgcg
    17641 cc
//
LOCUS       DAWBSB010000017        17178 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_868_length_17178_cov_3.588565, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000017 DAWBSB010000000
VERSION     DAWBSB010000017.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 17178)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 17178)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17178
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_868_length_17178_cov_3.588565"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4837..4846
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13335..13344
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3602 a         5593 c         4809 g         3154 t
ORIGIN      
        1 ttgcacttga tagtataatt caccatccct caaaaaagcc gggccgtttt tacggcccgg
       61 cttaaatatt ttcagcatca aaaatcggtc gttcctcccc ccaaaaacag ccctatgccc
      121 cgaggagcga gagacattgc gccagcgcgc acacggctgc cgtacaaccg aacacccgcc
      181 acggcatccg tttctccggc agcgcgcgca gcaccagcgc gcccgcggcc gccaaagcga
      241 aggcaaagaa gaaacccgaa cgcgcgcccg acgcccagat ggacggcgaa aagcccatgg
      301 cgccgtaaga cgcgaacccc gacagataga ccgcaacgcc cgcgtaagcc gccgccccat
      361 gccccagcgc caggtacagc tccagcgcgc aggcgaacag cacagcgcac aacagtaaaa
      421 acggcagcca gcgtttcagc gtccacgcgt tgagcggtgt gagcgtcccc ttttccgtca
      481 ccgcatcggc aaaaaacgac agacgcggcg ccagcgcacg cagcggcccg cccagcacac
      541 ccagcaccag cgcggcactt accgggaaca ggctgaacag ccggtaaagc ggctggcggt
      601 accgcgccca gacggtgcac gccagaagca cgcagaatac aaagaacaga agatgcggct
      661 ccagcaccat ccgggacatg gcagcagaga tccccagctc aaaattctgc cacagggaac
      721 gcatgccgta atcctgaaac caggatgtca cctcattgcc gtaccgcagc gctgtgcccg
      781 gacaggtgag tgcataaacg atattggccg cgcacagccc aagctgcgcc cacgccagcc
      841 aatggggccg gcgatgcgcc cacaggcacc aggccaggta tgccagcagc agaagcgtca
      901 gcagacccgc cagcagctcc atgttggcgg cgtacagggt aagcggtagc gccgcggcac
      961 actgccatgc ccgcggcctg tttccccgca gagattcccc cagcggcagc agcgccgcca
     1021 atgccgccag caaaggccac acgaacacca gcgtggtaca cacccagcct gcgctgctca
     1081 tatcctgcca cggatagcac agcgtcagcc cgcaaacggc ccagcggccc gcagggcggt
     1141 cttccaaccg cgcaaggcgc acgatgcacg cggcgcacag cgtgatgcac agagggtccg
     1201 ccaagcgcca cactattgcg ggcagcgcct ccacgatgca caccaccgcc tcgatcagcg
     1261 tccgggccga ccaggtatag taatgccaca gcgaaaaata cgccagcgag tattggtcca
     1321 gcatggcggc atacatagga tcgtcaccgg tgcccagcgt aaattgcagg tgcagtgcca
     1381 gcaataccgc atacagcgcc acatacggaa cgattcggct gcgcacagcg cggcccaccg
     1441 accgccgaag catgttcatc ccgtcacctc cgctgttttg cccgtggagg ccaaaaagtc
     1501 aaagccgccg atgcggtagg cgatacagat ctcatattcc gcaagcggat gtgccagcac
     1561 cttttccgga acgaccgccg tgaaaccgcc cagggcatag ccgcgcccgt ccgccactgc
     1621 cgcggtggcc gcttcatccg gcgtcatctg cgtgggaata ctgtaataag cgccgtcttc
     1681 cgcatgatac agtaccacac ggatatccac atctggaaac ggctctccct ccacacaggc
     1741 ccagcctgtg aacacggcgc ggccatcctc ccgcgacacc gccgcttccc agcggaaccc
     1801 ctccggcgcc gcgcctggat aatccgccgg gtccaccggc ctcaccgccg tcagcgaatg
     1861 gtacagcgcc agacaaaagc ccgccatgcc cgccagcacc agaacagcca gtgccagaac
     1921 cacacgcggg cgtgcgggta caaagccgtc ggggcccggc gcaggcgcac ggcatgtttc
     1981 cttttgcgcg ttcccgtcca tcgggacact ccttttttca cagtatggat gcggccatcc
     2041 agccgcgccg ccgctctatt tccggccgcc gaacagattt ttgaaaaaat tcaccgggcc
     2101 gccatagccg ttggccttat cgtttttcag cttgtccaca cactcccagc gcgcgggcct
     2161 gccgtcaaac acaagccccc cctgccccgc gcccgtcagc tggtgcggca cctcgtcccg
     2221 ggcgttggtg acattgcatt ttgaaaaatc ccattgaggc acgccggtat cgtccgcgcc
     2281 cggcgcccac agcttgaaca ccaggcccgc tgcttcgcca taacagaagc gctcgaacag
     2341 caggcggccg tcccggtata ccgtgacgcg gtcgcggtag cgctggtgcc cggcgcgctg
     2401 gtaccggaag cccgcgcact tcgcggcctt cacatcggcg atgatgggtg caaaatccat
     2461 actatatttc cccttcctgt tgccgcccct gcgggggcgc gttttcaaaa cggcgcgtgg
     2521 cctcgcgctc gcgctgcagc tcttccagca gtgccagcac gctccatgtc ttgttgtgct
     2581 tcgtcacaac ggctttcagc ggaacgtgca cgcccgcttc ccgcagtgcg tccagcaccg
     2641 cattgagctg agggctggtg aaattgctca tgagaaggag ctgcgcatcc ggcgcttccc
     2701 cactgtaaag cggagcggct ttcccgccca ggcccgccaa agcgcccacc ggctggagca
     2761 gctgctccgg cgcgatctcc ttggatgaaa agccctgttc ctccaacgcc gcgcgcacgc
     2821 cccggcccgt ttccgtggat tcgggcagac cgtacagcag cgccagcggc cgggccgcct
     2881 gttggttgat gtgtgctttc attcgttttt cttcccttcg tccagcattg cgctcaacgc
     2941 gcggcggaaa gcttcgtcgt cggccggcgt acgctttggc acgggcgtat aatgcccgcg
     3001 ccgccgcgct tttccgtttt catgccgtgt gtataacagt tgttctatat taccgtttgt
     3061 ataaagaagg ccgttctgat tcaagcatcg cgcgccgcgg cccagagcca tggccgccac
     3121 gccgtagtcc tgcgttacca ccaaatcccc ggccccgcac agcgttacaa gcttgaagtc
     3181 cacgctgtcc cggccttttt ccaccgtgac cacagcggcg taatcctcgc gcagcacatg
     3241 gctcgtgtcg gtgagcatgg tcaccggcac gccgcgccgc ctggcctcct ccacgatgat
     3301 ccgcttcacc gggcaggcgt ccgcatccac cagcaccgtc acagcgcgcc ctcctccccg
     3361 ttcgtccgca gcgcggcgtc cacccggcgc aaataataaa cgcgggatga gaaatccggc
     3421 atcaggcgca cgttttcatt gtagcccgtg ccggtgaacg cctgcttctg ccggatgccc
     3481 gcgtacatga gcagatcgcc gtaagcagca tagcgttccg tctcgcaggg catcatgtag
     3541 cggtcggcca ccgcgtgcat caaatggccg cccgcattca ccttcacagg cagtatctgg
     3601 ttgataaggc tgccgaaggt acgcacgtcg acgacctgcg gccgaacctc cagttcgtac
     3661 agagcatcct gcgcaaggcc tgccagccgc aggggcggct tttccgcgtt gggccgcagc
     3721 agacgcaggt actcgcccac cacggcctcg cttccatccg gcgcaagcat catccactcg
     3781 catttgtttt ttccgaacgg cgggcacagc cgcaaaaaac ggccgtactg gaacaggcgg
     3841 cggtgcgcct tataataagc gatctgcgcc gccacggcct tctgctcggc cggtgtgagc
     3901 cggccaagat ccagctcata gcccagcaca ccgaaagccg ccacattaaa ccggctctcg
     3961 atggggctgg tgcgcgccgt ctggtggttg ggcgaagccg agacatgaca gcccatcacg
     4021 ctgggtgggt agccataact ggtgcccgcc tgaatgagct gccgctcgta agcgtctgta
     4081 tcgtcgctgg tccagatctg cggcatataa cacaggatgc ccagatcgaa ccggttgccg
     4141 cccgaggagc agccctcgaa cagcacatcc gggttttgct ccacgatgct ttgcagcacg
     4201 cggtaaagcc ccaggatata gccgtgggaa aagcgtccct gctccgccag agcggggctg
     4261 taattgtcag agatatgacg gttcatatcc catttgacat agtcgatggg cgcgcttgcc
     4321 agcgtggcgt tcacgttcgc aatgatatag tcctgcacct cggcgcggca cagatccagc
     4381 acatactggt tgcggcccgt ggcgggaaca ttgcccggcg tttggacgat ccagtccgga
     4441 tgggcgcgga acaggccgga atctgcgttc accatctccg gctcgaacca caaaccaaac
     4501 agcagcccca gagcgtgtac cttttccgcc aggcctgcaa ggccgccggg cagctttttg
     4561 cggttgacgg tgtaatcgcc caggcccttt gcgtcattgt cccgtgcgcc aaaccatccg
     4621 tcgtccagca cgaacagctc cgcccccagc ttcgccgcgt cctttgccag tgagagcagt
     4681 ttgcgctcgt taaaatcgaa ataggtggcc tcccagttgt tcagcagcac ggggcggtct
     4741 ttttttgccc atttgccgcg cacgatgtgc tcctgcacaa aagcatgcag gtttgcgctc
     4801 atgccgtttt tgcccgcccg gctgaaggtg agcacgnnnn nnnnnngcac ggcttcgggc
     4861 gtttcaaacc gttcaccggg cgccagcgtc catgcaaagc cttcgggctg aatgcccgcg
     4921 gccacgcgcg ttttccccca ggggctcacc tccacacagc ccatatggct cccggaataa
     4981 acaaggttga agccatatgc gtcgccgttg aattccgtcg cgtccggcgc gcacagcatg
     5041 aaaaacgggt tgcaaaatgc cgacgacgca ccggtggtgc tgccgaacac atgcgctccg
     5101 ggcgtaagcg gaacgcacgc ttcgtggcgc tcccgcgccc acgcgcccgg aaatgtggag
     5161 agcacataat cgcagccggg caggtccagc tgatagctga gcgccttttc cagcgtgaca
     5221 ggcgcgtctg cgccgttttt tatacaaagg cgccgcgtga tgacgtcgca gtccgggtac
     5281 acggtataaa acaacgtgac agccacgccc tccggcgtgg aaaagtccag cgccagcgtc
     5341 tctgctccgc cccgggccgc aggcatgccc tccggcggga cacttccctc cagcctgcgg
     5401 gcgcatacaa aggaaaaatc cgccgtacgc gcgccgcccg gcatcacgag cgaaaggctc
     5461 tgggcacgat aatctccctt ttgcagcggc gacagctcca gacacagatg ctccagcgaa
     5521 agcggtgcgc tctcagcccg gtacaccacg tcgccaccat atccgatatc cgttttttcc
     5581 cgcaggggca cggaattttc cacgcgtacc cgcgcgccgt aatgcaggtt ttccacaagg
     5641 ccaccgcgca cgcccagata atagcccgtg ttggccgttt ccagcgcgaa ccagtccccg
     5701 tttactgtga tcatcttccc gcctccgccg tttattttgt ttcttttctt tcttattatc
     5761 tattatgctg gttttctttg ccttcgtcaa tgtctatttt gagcgatttg tcaataaagc
     5821 agcacaaaac tccatgcact ttgccaatag aaaaacacgg tgcaagcggt tataataaag
     5881 acagaaggaa aggagtgagt cccatgtact cgactgaaga acccgtgcag tcggtgcagg
     5941 gcgtgtaact cggacgaacc gctaaagtca gattcgatta gatgcagttt ggtgcccggt
     6001 gttgcgggac gagaaaccgc aggagatgcg gcagccaaac gtttttagca aggtttcgtc
     6061 tcaagaggat atgcgccttg cacggataaa aagcacagcc agtggctttg cgcaaagcgg
     6121 ctttgtaaag aaagagctct atgcaggttt tgagatcccg gcaaaagaaa cagcaatgat
     6181 cctacgaaag gcgcctttta aaaagacggc attccggaag caaagcgaat tctttccaag
     6241 gaatacggtt tcaaccaagc aaacgatctg gtaaaacaaa ataaatacga cgaaaggtat
     6301 tggaaccaat gcactaagca agcgttctcg ggaacttttt caaaagtaag agatacagaa
     6361 gcctgtgttt cggaaatacg ggaaaggaac caagacccat gcagttttaa agcggggtgt
     6421 ataccccaaa tgcttccaaa aacgtaagtt gcattgagaa cacaacaaaa catggagccg
     6481 cagcgcctgt tggcgctgcg gttttttcat gcccggataa cgcgggcacg gcggctctac
     6541 cgctttgccc gtttcctttt ggcagcccgc actgccgcac ccaccgccat accggcaacc
     6601 gcgcacagca atccgggcag aaaggcggac agctccggca gattgaattt ttgaaacgag
     6661 gccatattgc tcaggctgaa cgtcacataa ggccccagca tgctcatcgc tccaaaaaag
     6721 aacagcattg tccgtttgca gccgtcccac gcccggccgc atccgatgaa aaaggacagc
     6781 accagcgtgc tcaacgggag aacggcataa aacaccacca gcgagtaccc cattgcatcc
     6841 gttctgccgc ccatccaaaa cgcaagaaca gacacccccc agaccacaag atacaccgta
     6901 accaaaacaa accgtaaaaa cgcccggcgg cttttcaccg catccttgct ttcctcccgg
     6961 tgcgccagca aagacgcgtc gtctctttcc tgtgtctggc ccgcctccag ccgcgcgccc
     7021 ttcagtttct gtgcaggctt cattttaccg cctccctacc ccttttaagg tacggcaaaa
     7081 gcatttcaaa tacaagcaaa tccctattcc attccatcgg gacaggtgtc aaatcccacg
     7141 ttacagcagc ctgcgcagca cagaatccgc cgccagcgca tgcaggctgc acgccacatc
     7201 ctccatgcgg tgcgcgtccg acgaggacag cacctccagc ccagcgggca gcagcccttt
     7261 ctccaccagg ccgcccaaac gggaagcgtc gtgcagctca acggctgcaa agggcagatc
     7321 ctccggcaaa aaaccaagta ctgaaagcag cgcgtagctg tccttttcca catgcgcggg
     7381 cacagcaacg cccccaagtg cctcgcaggc ggcctttacc tcgtaaatat ccatcgacac
     7441 cgcgcccgtc agcagtttgt ccacagtgcg cagcacttcg tcgttttcat ccatcaccag
     7501 ctgacggccc catacggcgg gatcatacgg gaactccggc agcgcggcat acagcaagcg
     7561 gccgaattcc agcgctgtat ccacggtttt gaagtaacac aaaacatgaa tttcttcggc
     7621 ggtatttacc tcgacgcccg gcagcaggcg caggccgtat gcgttgcagc agcactgtac
     7681 cgccggcaga ttgtccgcgg cgttgtggtc tgtcacagca atcagctccg cgcccgccag
     7741 cttggccagc ccggcgatgt tcgcgggcgt catatcgtcc tcggcgcagg gcgaaagaca
     7801 ggaatgcata tgcaggtcat acagcaaaac cccatccccc gctttctttc acagtcggag
     7861 taaaagcgct gtcagccttc tccgtcctcc acgaaaaaca ttcccgccgc cagtgcgatc
     7921 acgccggccg ccactgccgc aatgggcaga tgatggacga caaattcact gtatacatag
     7981 ctgatgctcc aaaggcggaa aaatatcccc accaggatga gaccgatgcc cagcagcatc
     8041 actttgaccg agcgcatgct ttttccttct ttcttcacag cttcattgcc ctggccagcg
     8101 caaagccggc ctcaaactcg ggcagatctg tggtgaaaat attgattccc tgttcttccg
     8161 cccgcgtttt cgccaccgcg tccagcgccg cgccatggca tagaacgatg caggcgcagt
     8221 ccgccagggc agccacggca accgcattta cattgcccat caccgtgaac caagcgtcgc
     8281 cttccttggc ccggcccatc gcccagctca gcaggtcgcc cgtaaaaatg cccgcgatct
     8341 cccgcgtctc tgcctccaca gcgggcgcga agccgcacgc agcggaaagc tcttttgtcg
     8401 tcatcccgtc actccccttt tcccagcgcc ttgagcacgg cctccatgcg gcggcgcatc
     8461 aaaacagtgc agtcgtttac ggcagccttg ccccgcacta cgtcctcggc cagcgcggca
     8521 caggtgggcg ccccgcagct gccgcagtcc agccccggca gctcggccag gatctcctcc
     8581 atctggttgt agcgctcaaa ccgttcggta cgcgtaccgc ccagctccag tacgggagca
     8641 taggtaaggt ctgtgtccca cagcatctcc gtgggaatgc tgcccgaaag atggttcttg
     8701 ctcacaggca cgtaacgccg caggcgtttg atcttcgctt ttgcaatgta cgggttttcc
     8761 acctgcagca cgccgcccac gcagccgccc gcacaggcgt ccagctctac aaagtccagg
     8821 tcccggtatt tttcatcctc caggtcctcc agcacgcgga tgacgttctc gatgccgtcc
     8881 gccgcaagat agctgtcggt ggagatcagg cccgcggcct cgccgccgct ctcggcccaa
     8941 cccaggccga tgcgtccggc gctggccaga taatccatct gcgagatacg cttcatggac
     9001 gccagcagac gcgggtacac ctccttgatg gccaccgcgc catccacctc gctgcgggac
     9061 gagccgatgg gcgaatggat ggccgtcacc ttggccgggc acggcgtgat gaaaatgcag
     9121 ccaatgtcct ccttcggcag gccggttctg cgcacggcct cttttttggc caggcgcgcg
     9181 gccagctcga tgggcgcgac cagcggcagc acatggtcga tgagctgtgg aaagcgcacg
     9241 cggatgagcc gcgtcaccgc cggacaggcc gaactgatca caggccgttc gatgctgcct
     9301 gtctgcagaa tgcgtcgtgt ggcgtcgctg acaagctccg cggcctttgc cacctcgtac
     9361 acatcgtcga agcccatatc caaaagtgct gccagcacca catcctgctc ctccaaattg
     9421 ttatactgtc cgtataaact cggtggaggt aaagcaattg tatatttgaa atcatccaaa
     9481 atcgtcaacg ggtcatacag cggcttcttt gcatgatgcg gacagatgcg gatgcactcg
     9541 ccgcagtcga tgcagcgctc ggccaggata tgcgccttgc cgtcgcgcac gcggatggcc
     9601 tcggtggggc agcgcttgat gcagttgatg caccctttgc acaggtcctt gtccagggtg
     9661 accgagtgaa tgaatttttc caagcgtgcc gcctcctttc ctcagatgct ccgccgcgcg
     9721 cggcggcggg cctcaaataa aatacaacgc cagcagcgcg gcgcctgccg cggtaagcac
     9781 cacagcccat atgaaatcgg cacgcacagc gtgcatcgga gcggcgtctc cgtccagcgc
     9841 atattcgccg gggcgcgcag ggtcatacag cacctgcaca cgctggccga tgcgccacgc
     9901 tccttcccgg ctgcctccgg tggcgatcca ttcagtctcc ccgccgttca cggtgaaccg
     9961 gacgcagggg taccagttcc actgcgtcat cgcctgcggg atactgagaa agccatgtgc
    10021 accgttggct gtagtgcggc cccagtactg gtacgccact ttgggaacat cgccctgctt
    10081 ttcataacgc atcggcaggc ggtggcgcac aaggccgcac accacgccct ccgctttggc
    10141 cggcgcccgg cgggcgcggc gaagcatcag cgcctccaca aagccccaga cggcattgaa
    10201 tatgccaagt acaacgcacg cggctcccaa taccatgatc cacaacataa ggcatcctcc
    10261 tttcggccgg gccgtatcat acggccgtcc gctttgaaac gtttctttcc cgcgtcttcg
    10321 cacagagcgc cgcatcaagg cacggctgct cccgcagcag cggccggcag caccgcagcc
    10381 ccacacacag aataacgcct ggagacacgg ctgcgtttca ccccgcaacg aaaaacgtcc
    10441 ctcagtcgat gttcacaagc atcttcaccg tggtgcccac gcctacggtg gactggatgt
    10501 caaattcatc tgaatatttt ttcatattgg gcaggcccat gcccgcgccg aagccgaggc
    10561 ttcgcacctc gtcgggcgcg gtgctccacc cttcgctcat ggccagggca atgtccggga
    10621 tgcctgggcc ctgatccgtc agcgtcatat ccactctctc atcggtaacg gccacgtcga
    10681 tctcgccgcc gcccgcgtgg atggccaggt tgatctctcc ctcatacagt gcgatggcaa
    10741 ccttacgcac agcgtccgca gggatcccca gcttcttcag ccgtgccttc aaatcggcgc
    10801 tggcctcccc cgcacgggtg aaatcgtccc cgggcacggt ataatgcagg cggatcgcgt
    10861 ccatcacgcc atccccccgc gcagcccttt ttcgtacagc aggccgcacg ccgcgaacat
    10921 gggcagcttt gtagccaaca cggcgatgcc ccggtccacc gccagctgca ggatggcgcc
    10981 gtccggcttt ttgccgcgca caaacacgat gcagatcatg tccatcatgt ccgcggtgcg
    11041 caccacctgc gggttgttca ggcctgtgac aagcacggcc tggtctttca cataggccag
    11101 cacgtcgctc atcatgtcgc tgccgcaggc cgcgtgcacc tcacgggtga ggtccgcctc
    11161 cggcgtcagc agctcggcgt ccagcgtttc cattacttgc ttgatggtca ttgcgcttcc
    11221 actccatttc tgcccgcgcg gctgcgcggc ggcataagca gccccggtga caggggctca
    11281 ccttaagtat acgggaaacc cgttccgaaa acaagcgcgc cgggccgctt tgccgttgtg
    11341 tttctgcaca gttttttacg cataaattct acatctgcgc tataaagtag cagccgctaa
    11401 cttgtcgttt ttttaacaaa tttctgttat aatcaaatac aactgggcca aagcacggtt
    11461 tgtttgcccg tacctggccc aacttacaaa cgggaggact atatatgagg cgcttttcca
    11521 ctgtgccctt caagggcaca aaagagcagg aagccgaact ggtggcctgg ctcgaggagc
    11581 acaaagcgac gcccggcgtg gccatgccgg cgctgcagaa ggctcaggag atctatgggt
    11641 atctgcccat cgaggtgcag cgtatcgtgg ccaatacgct gggcaaacct ctggcggaaa
    11701 tttatggaat ttccacgttt tattcgcagt tcagcctttg tccgaagggt gaatacaaaa
    11761 tcagcgtttg tctgggcacg gcgtgctatg taaagggcag cggcaagatc tacgagaagc
    11821 tgcaggaaaa actgcatatt tcgggcggcg aatgcacgga agacggcaag ttcagcctgg
    11881 atgcatgccg ctgcatcggc gcgtgcggcc tggcccctgt catgacgatc aacgacgatg
    11941 tatacggccg cctgacgggc gacgaactcg acgagatcct ggcaaaatat tgagcttata
    12001 cttatgttag gatgaggtaa gcacaccatt caccgcacca caccggacac gaaaggcggg
    12061 cgccgatatg caggaacttt caatgaacgt tctggacgtg gcggaaaatt ccgtcgcagc
    12121 cggggctacg ctggtcgcca tcacgctttc cattgatacc gcagcaaaac gcatgacgct
    12181 gaccatcacg gacaacggca agggtatgcc gccggagatg gcggcccgtg tgacagaccc
    12241 gttctgcacc acgcgcaaaa cgcgcaaggt cgggctgggt ctgccgtttt tgaaaatggc
    12301 ggccgagatg acggggggcg cgctttccat cgaaagcaca gttggaaaag gtacctgtgt
    12361 aacggcctgg ttcacactgg gccacatcga ccttgctccg ttgggcgata tgtccgccac
    12421 ggtggcgggg ctcatccagt gcagcccgga catcgacttt gtgtacaccg tgcaggccga
    12481 cggcgagcag ttcgccgcgg acacgcgcga gctgcgtgcg gtgctggacg gcgtgcccct
    12541 gagcgagccg tccgtagcgc tttggctgcg ggaatatctt gccgagaaca ccgaacaact
    12601 tttacaaaag gagaacgctg tatgaaaagc ttagaagaac ttgccgccat ccgcgacaag
    12661 atgaaacaga ccgtgaatac gcgggaagcg gcgcacgatt ccacgcgcgt cgtggtcggc
    12721 atggccacct gcggcatcgc cgcaggcgcg cgccctgtgc tgaacgcctt taccgaagag
    12781 gtcgcacggc gcgacctgca cggcgtgctc gtcacccaga cgggctgcat cggcatctgc
    12841 cagtacgagc cggtggtgga gatcacctgc cccggccagg aaaaggtcac ctatgtaaaa
    12901 atgacgcccg agaaagtcgc gcgcgtcgtg tccgagcatc ttgtgggcgg gcatcccgtc
    12961 accgagtaca ccatcggcaa cgtacggtaa ggaggagaag caatgtttag aagccatgta
    13021 atggtctgcg gcggcaccgg ctgcaccgcc tccggcagcg ataagatcgc gaaggccttc
    13081 gaggacgaga tccttgccac cggcctggaa aatgaagtca aggtcatccg cacgggctgc
    13141 ttcggcctgt gcgcgctggg ccccatcgtc gtcatctatc ccgagggcag cttttacagc
    13201 atggtcaagg aagaggacgt taaagagatc gtggacgagc acctgctgaa gggccgcccc
    13261 gtcacgcgcc tgctgtacga cgagaccgtt gccgaggaca acaccgtcaa gagcctagac
    13321 gacaccgcgt tttannnnnn nnnnctgcgc aactgcggcg ttatcaaccc cgagaacatc
    13381 gacgaataca tcgccttcga cggctatcag gcgctgggca aggtgctcac tgaaatgacg
    13441 ccggacgacg tcatccagac gatcctggac tccggcctgc gcggccgcgg cggcgcaggt
    13501 ttccccaccg gccgcaaatg gcagcttgcc tcgggcaacc gcggcaatgt gcagaaatat
    13561 gtatgctgca acgccgacga gggcgacccc ggcgcattta tggaccgcag cgtgctggag
    13621 ggcgaccccc acgttgtcat cgaagccatg gccatcgcgg gctatgccat cggcgcctcg
    13681 cagggttatg tgtacatccg cgccgagtac cccatcgccg tgcaccgtct gcaggtcgcc
    13741 atcgatcagg cgcgcgaata cggcctgctg ggcaacaaca tcttcggcac cgatttctgc
    13801 tttgacctgg acatcaagct gggcgccggc gcctttgtgt gcggcgagga gaccgctttg
    13861 atgacctcca tcgagggcaa gcgcggcgag ccgcgtcccc gtcccccgtt cccggccgtc
    13921 aagggcctgt tcggcaagcc caccatcctg aacaacgtgg agacctatgc caacgtgccg
    13981 cagatcatcc tgaagggcgc ggactggttc gccagcatgg gcaccgagaa gagcaagggc
    14041 accaaggtgt tcgcgctggg cggcaagatc aagaataccg gccttgtaga gattcccatg
    14101 ggcaccacgc tgcgcaccat cgtcgaggag atcggcggcg gcatcccggg cggcaaaaag
    14161 ttcaaagccg cgcagacggg cggcccctcc ggcggctgca tcccggccag tctcatcgac
    14221 acacccatcg actacgacaa cctgcttgcc atcggcagca tgatgggctc cggcggcctc
    14281 atcgtcatgg acgaggacac ctgcatggtg gatatcgcca agtttttcct ggaattcacc
    14341 gtagacgaaa gctgcggcaa gtgcacgccg tgccgcgtgg gcaccaagcg tctgctggag
    14401 ctgctgaaca agatcacgga cggcaagggc accatggagg atctggacaa aatcacagag
    14461 ctggccgaat tcatcaaatc caacagcctg tgcggcctgg gccagacagc cccgaacccc
    14521 gtcctctcca cactgcgcta cttccgcgac gaatatgtgg agcacatcgt caacaagcgc
    14581 tgcccggcgg gcgtgtgcaa ggcgctgctg aactacaaga tcgaccccgt caagtgcaag
    14641 ggctgcacgc tgtgcgcgct cacctgcccg gccggcgcca tcagcggcac cgtgcgccag
    14701 ccgcatacca tcgataccgc caagtgtgtt aagtgcggcg cctgcatcga gaagtgcaaa
    14761 ttcggcgcca tcagcaaagg ttaagagagg gagtgacaac gaatggaaac cgtacatatc
    14821 aagatcaata acgtgcccta cgaagtcgct gccggctcta ctatcctgga agcggcccac
    14881 agcgtgggca ttgagattcc gaccctgtgc tatctgaaag agatcaacga gatcggtgcg
    14941 tgccgtatct gcgttgtgga ggtcaaaggc gcgcgcagcc tggtcaccgc gtgtgtgtac
    15001 cccgtcaatg aaggcatgga gatcgtgaca aacagcgaaa aagtgcaggc gtcccgcaag
    15061 acgaacctgg agctgctgct ttccacccac gaccagaact gcctgtcctg cgtgcgctct
    15121 accgattgcg agctgcagaa gctctgccgc gactacggcg tcaacggcat gcgcttcatc
    15181 ggcagccgcg agcgctatgc catcgacgat tccgcgccgc atatggtgcg cgacgataac
    15241 aagtgcatcc tgtgccgccg ctgtgtggcc gcctgcaagg ccaaccaggc cgtgggtgtg
    15301 atcggcgcca acgagcgcgg cttctccacg cacatcggct gcgcgtttga agcgcctttg
    15361 ggcgattccc cctgcgtttc ctgcggccag tgcatcaccg tgtgtcccac cggcgcgctg
    15421 acggaaaaag acgacaccgc caaggtgtgg gccgcgctgc gcgacccgtc caagcatgtt
    15481 gtggtgcagc ccgcgcccag cgtgcgcgcc accttgggcg aatgcttcgg catgcccatc
    15541 ggcaccaacg tggagggcaa aatggtcgcc gcgctgcgcc gcctgggctt tgacggcgtg
    15601 ttcgacaccg acaccgcggc cgacttcacc atcatggagg aggccaccga gtttgttcag
    15661 cgcgtgaaaa acggcggcac gctgcccctg atcacaagct gcagcccggg ctgggtaaaa
    15721 ttctgcgaaa cctattatcc ggacatgatt cccaacctgt ccagttgcaa atctccgcag
    15781 cagatgttcg gcgcgctgac caagacctac tatgccgaaa agatgggcct ggacccccac
    15841 gacatcgtgt gtgtgtccgt aatgccctgc accgcgaaga agttcgagat cggccgcgac
    15901 gacatgagcg cagcgggcga caatatcccc gacgtggata tctccatcac cacccgtgaa
    15961 ctgggccgta tgatcgagcg cgcgggcatc aagtttaccg agctgcccga cgaggatttc
    16021 gacccggcgc tgggcgagag caccggcgcg gccaccatct tcggcgctac cggcggcgtg
    16081 atggaagcgg ccctgcgcac ggctgtggag cttgtgacgg gcgagacgct ggagaaagtg
    16141 gaatttcacg acgtgcgcgg caccgacggc atcaaggagg ccagctacga tgtgggcggc
    16201 ctgcacctga acgtgtgcgt ggccagcggc ctcaccaact gcgacgcagt gctgaaggct
    16261 gtgcagagcg gcgagaagca ctacgacttc atcgaattca tggcatgccc gggcggctgt
    16321 gtaaacggcg gcggccagcc cacgcagccg gccagtgtgc gcaactttac ggacctgaag
    16381 gccctgcgcg ccgcagccct gtacggcgag gatgacgcga aggccatccg caagagccac
    16441 gagaacccgc tgctcaagaa agtgtacgcc gagtatctgg gcgagccggg cggccacaag
    16501 gcgcatcata tcctccacac ctcctatgta gcacgcgaaa aattgtatta agcaaacgcc
    16561 tgcgcccgcg tggaaatttc cgcgcgggcg taatttttca atttttcctc tcgttgtaca
    16621 tacaatgagc cggaggcata tcctttttat ggaagggagc ttagggatga agcatttttt
    16681 ccgctttttt gcagcagccg cctgtacggt gctgctcctc gcgctgtgcg cctgcacgcc
    16741 agacggtaca tcctccccgg tatcctcctc cgcgacggaa ggcgtttcct ctgaaagcgc
    16801 ggcgcaaagt attcccgccc ctgaaagcgc atcgggtgag ggtgcacagg cgctttcgct
    16861 tctgcccgcg gacgcggcga atatccagcc ggatgctgtg cctgaatttc tcaccgcaga
    16921 ccaacaggag ctttaccgcg cggcctatta tctgtattat cactttgatg tatccagtgg
    16981 atttgctttt gatacgatcg atgaaaactc tccctttatt gtcggagata ccggacggag
    17041 ctactatcaa gataccgggt ttgccaatta ttccgccttt cgcacagcac tggattgcgt
    17101 gtttacgtca aactttgccg acagtcttat tgacaaatat caaaactata tcaagggcga
    17161 tgacggcctg ctttattt
//
LOCUS       DAWBSB010000018        17066 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_873_length_17066_cov_3.255776, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000018 DAWBSB010000000
VERSION     DAWBSB010000018.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 17066)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 17066)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17066
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_873_length_17066_cov_3.255776"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    8370..8379
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    14769..14778
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3393 a         5546 c         4527 g         3580 t
ORIGIN      
        1 atttgcaaat ccagctcgta tgcgataacg catttacatc attcagctga atcggataag
       61 cagaagcagg cgctttcgca ccggctcgcc tgttatggcc tgtgctgcgg tatgagccag
      121 cagcttgtct gcgatcgggt ctggacttgt agctttcctt cctattgtgt atcctccctt
      181 tttgccttcg accgtaccag aagactgctg cagaaattaa aaaagagcac ttctcagatg
      241 aactctaaga agtgctctca tagcattgct aaacctgtag cttattcgtt tgaactccgt
      301 cattcttcat aacgatgcgt tattcttcaa caatttgctg tcacaatgct gtcatccaca
      361 gtttgacagc acagagctcc gcagcatata gggcaacacc gcacaattca cggctgatgc
      421 ctaagcgtcg tctcgcttgc agattttccg acaactgtca caaaaatctg tcggcgcggt
      481 tcgacactta gaattgtgcg gtgttgccat agttgttttc cattgcgaat tcaactatag
      541 attatggttt tatcagtcga tgggtttcat cgtggggaaa aggatgacct cgcggatggt
      601 gctggaattc gtcagcagca tcacacagcg gtcaatgccc atacccatgc cgcccgtcgg
      661 gggcatgccg tattccagag ccgtcaggaa atcctcgtcc agcatctcgg cctcgtcgtc
      721 gccgcgctcg cgctgctccg cctgcttgac gaagcgctcg cgctggtcga tggggtcgtt
      781 cagctcggaa taggcatttg ccatctcact gtggcaaata aacagctcga agcgttccgt
      841 caaacgcttg tcctgcgggc tgcgctttgt cagaggagat acctccaccg gatacattgt
      901 gatgaatgtg ggctgaatga gcttttcctc cacacgctgg tcaaagcagg cgtacagcgc
      961 gttgccccaa gtgggctccg ccgcctccgg cagctccacg ccgatggctt cggccgcggc
     1021 aaccgcctgg gcattgtcgg agatggcgcc gaagtcgatg cctgcatact ccttcacggc
     1081 gtccaccata gtcaggcggc gccagcccgg cgtaaggtcg atcttttcgc ccagccactc
     1141 cacctcatag ccgcccagca gctccagcgc ggccgaggaa aggatcccct cggcaatgtc
     1201 catcatcgta tggaaatccg catacgcctg atacaactcc acggtcgtaa attccgggtt
     1261 atgcttcggg tccatgccct cgttgcggaa aatacggccg atctcataca cgcggtcgat
     1321 gcctcctacg atcagccgct tgagcggcag ctctgtggcg atacgcatat acatatcaat
     1381 atccagcgtg ttgtggtggg tcacgaaagg acgcgccgca gccccgcctg cgatggtgtt
     1441 cagcaccggc gtttccactt ccatatagcc gcgctcatcc aggtaattgc gcatatggcg
     1501 aataaatttg gagcgcacct caaacgtctt gcgcacctcc gggttgacga tcaggtccac
     1561 atagcgctgg cggtaccgcg cctcgcggtc cttcaggccg tggaattttt cgggcagagg
     1621 cagcaggctc ttgctcagca gtacgatctc ggtgacatgc acgctgattt cgcccatctt
     1681 cgtacggaac acaaaacctc tgacgccgat gatatcgccg atatcaaatt tcttgaacgc
     1741 ctgatactcc tccacaccca cgtcgtcccg gcggacataa agctggatgt tgcccgtggt
     1801 gtcccgaagg tccgcaaagc tggccttgcc catcacgcgc ttgctcatca tgcggcccgc
     1861 catgcacacg ctgcggccct gttcaccctc gggcacatcc acaaagctct ctttcacctc
     1921 ggcggcaaaa tccgcctggg gatagcttgt cagctcaaag gggtcgtttc cggcctcctg
     1981 caagccgcgc agcttttcgc gccggatggc gaccagctcg ttatacgcct gctcactcac
     2041 ttcttcggct acggccccga tcttctgctg ggctgcctcc aacgcattgc ccaactgttc
     2101 caacgtttcg tttgccatgc ttgtcctcca acatctcgca atttatccca tccagtatag
     2161 cacaaaatat aacacaaaaa caggtgcttt cgcacctgtt tttgaaaaaa gcctgatttt
     2221 gtgcggctta cagctctgct cggccgatct ccatcacttc gtactgcaca actgcgccgc
     2281 tgggcagcgt gatatccacc ttatcgccca cggaatggcc aataagggcc gctccgatgg
     2341 gagattcgtc cgagatcttt ccgttcatcg ggtcagcctc ggtggaaccg gtgatatcgt
     2401 attcgtcttc atccccgtcc tcatccttga tgcgaacatg gctgccgacg ctgatatggt
     2461 cggtagaaag ctcgtcctcg gtgacgacgc gggcatgctt gagcattttt tccaaaagcg
     2521 cgatgcggcc ttccacaagg ccctgctcgt ttttggcttc gtcatattca ctgttttcgg
     2581 aaaggtcgcc gtagccgcgg gccactttga tcttttcggc cacctctttg cgcttgacgg
     2641 ttttcagctg ttcaagctcg tcctccaatg cacgcaagcc ggcataggtc atgacaattt
     2701 ctttttccaa ggtctccggt ctccttcaca gatattggta ccgcgccgcg ccacagtaca
     2761 aaatgtgggg cacttgtaca atatgggtta ttatagtgtt tttgcttttg gctgtcaagc
     2821 ggaaaaccaa aattatacaa attgcaccag taaatgggga tttctttgta tattacggct
     2881 gaacagacat tttttgtgag cctgttccgt tcctttgtat acaatgggcg gcggcagacg
     2941 ggcgtacaca aacaccgtcc tgctgtaaaa tccccctgat gctgctattt tatgcatcgt
     3001 ccggacaaaa gagaaccaca ggagggaaaa tcatctccct cctgtggcgg ttttccgctg
     3061 gtattgggac gttactcctc gtcgtccttc atatcaatga acttcttcgc ttcctcgatg
     3121 accttcggct ccaaattgga gggcagcggc tcatagcggg tgaattcaaa tacaaagctg
     3181 ccgcgtcctt gcgtgagctg gcgcagatag gtggtgaaat catgcatctc actgaccggc
     3241 acgtcggctt ccagcgtctg ctgaccatcc tcggccggat gcatgcccag cacgcggccg
     3301 cgccgctttg tcacctcgcc catgatgtcc cccagcacat cgcccggaac agtagccatc
     3361 aggttgccga tgggctccag cagcacgggg ccagcctcgg gcagcgcagc cttgtaggcc
     3421 agcttcgcag ccatcttaaa cgccatttcg gaagaatcca ccgggtggta gctgccgtcc
     3481 agcagcgtgg ccttcaggcc caccacgggg tagccggcca gcacgccgtg gcgcacggcc
     3541 tcctgcaggc ctttttccac cgccgggaaa tagttcttgg gcacgctgcc gccgaacacg
     3601 ttctcggcaa atacaaactc tccctcgtcc gtcggctcga attcgatcca tacgtcgcca
     3661 aactggccgt gaccgcccgt ctgcttctta tggcggccct gtgccttaca tttcttacgg
     3721 atggattcac ggtatgcgat acgcggctcg gtgaggctca cctccacgcc aaacttgctt
     3781 ttgagtttgg cgcacaccac atccagatgc tgctctccca aaccggatat cacctgctgc
     3841 accgtctcgg tgttgacggc gtaccccagc gtcgggtcct cttccatcag gcgctgcaac
     3901 gcgccgctga tcttgctctc gtcgcctttc tgctttacct tgatggccat ggacagcgta
     3961 ggccggggga actcggggcg cggcaggctc accacacggc ccggcgcaca cagcgtgtcg
     4021 cccgtctgcg cggcaggcag cttggtaatg gcaccgatgt cgcccgcgcc gatctccgcc
     4081 gcatccgtct gcttttttcc tttcacgaac accagcttgc ccatgcgttc gggctcgccc
     4141 gtgcggctgt ttaccaccgg agagtccgcc ttgagcgtgc cgctgacaac cttgacatag
     4201 ctcatcttac ccacaaacgg gtctgctact gttttgaata catatgcgca cagcgggtct
     4261 ttcgcctcgc aggcgatctc cacttcgttt ccgtcgcggt ccgccgcaac gcacccagcg
     4321 ccgcgcgctg cgctgggcag cagctttttg atgtagcgca gcaccatgtc cgtacccgcc
     4381 agcgcagtgg agcagccgca gagtaccggc gtgatggcgc ccgttttaac gcctacgcgc
     4441 acaccctccg tcaactcctc ctgcgtgaag ggctcaccct cgaagaattt ctccatcagc
     4501 gcgtcatcgg tctcggccac agcctcgcgc atggcctcga tcagcccgtc ctcccgatgg
     4561 ccgtatttgg gcataggcac ttcgcgggcc gtgccgttga cgtagctgta cgccttggat
     4621 tcgatcaggt tcacaaacac gcggccgccg gcctgctcga tgggcacgac caccggacag
     4681 atggacgggc cgaactccgc tttcagctct tcaaatactt tatagaaatc ggcatgctcg
     4741 gcgtcgattt tagagatgta gatcatacgg ctcttgccgt gtttcagcgc cagcttgtac
     4801 gccttctgcg caccgacggt gaggccgctg cggccggaca ctgtaatgag cacgctctcg
     4861 gcggcgcgga tgccctcgtg cagacccagc tcaaaatcaa acagacccgg cgtgtcgatc
     4921 agatttattt ttacgccgtc atactcgaca ggcgctacgg ccagcgacag ggatgcgcgg
     4981 cgcttggcct cctcggggtc gtaatcgcat acggtgttcc cgtcttccac acggcccagg
     5041 cggtcggtgc tcttggcggt aaacagcagc gcttcggcga ggctggtttt cccggcgccg
     5101 ccgtggcccg ctatgaggac gtttcggatg tgatcgtttg cgtaattcat aaacaacaac
     5161 cctcttttcc gccctgcgca aaacgcatgg cagcaactta tacaaaaata gtatcacaat
     5221 cgcgtcgctt tttaaagata atgttgtaaa aaaagaaaga atcttacgta caatcttcca
     5281 gcataagatt gtatagtttg acaagaggtt ttcaacaatg gctgcccggc gcaaacaata
     5341 agccgtcctc gtgcctgcgg cgcagacaaa atgcagaaaa acacataccg ccgcgctttc
     5401 gtcaaaaaaa gctggtatgc ggacgatgtg catgataaaa tgatgccaca aggcgggccg
     5461 gcaccaaagg cgcccgcgga acatcgagcc gggcgcgttc agccgtccgg agaaagggac
     5521 gcgttatgac ggcatatgaa aaaagcctgg aggtcctgcg cgagcttttc gaaaaggact
     5581 gcaccttcgt attggcgacc acgcgggaga acgttccttc gcagcgggtg gtggacgcct
     5641 gttatatggg cggcgcattc tgggtggtca cgcacggccg ttccgccaaa gtgcgggaga
     5701 tgaaagcaaa cccctgtgtt tcgctctgca gcagttttca cacctttaag tgcaaagccc
     5761 atttcatggg acatccgctg ctgcctgcca accaggcggc gcgcgaagcg ctggcggggg
     5821 cgttcgagcc gtggcacttt gcctgcaacg atgaaggcga ctccaacctg tgttatctgc
     5881 gcatcgtacc ggagagcggt tttttccaca aggacgggac aggctaccgg gtggatttca
     5941 tcaacgggac ggcggcggaa ttccccttta cagctcaggt cgaaatggct ttacagccgc
     6001 gcgcaaatat tccggtattc tgaaaagggc gggagcacac aaggctcccg cccttatatt
     6061 acatctataa aagcctcaga cgacaaaccc gccgttgggc gccaatacct gccccgtgaa
     6121 aaaagcgccc gcgtccgatg cgagaaacgc gacggcagcg gccacgtcct ccggcgcgcc
     6181 cagacggcac agcggcgttt cctctgcaag cgccgcctta tcctccgggg agaaaacagc
     6241 catcatatcc gtttccacta cgcccggcgc cacacaattt accgttattc cgcttgggcc
     6301 ttcctccttg gccaatgcct tggtaaagcc gataagcgcc gcctttgccg cggaatagtg
     6361 cacctcgcag gaggccccca cctgccccca catggaagaa atattgatga tgcggcccca
     6421 cttttggcgt atcatatgcg gcagcgcggc ctggcagcag tggaacgcgc ctttcacgca
     6481 tacgccgaac atggcgtccc actcagccgc agtgatatct gtgaacagct tctgctgtgc
     6541 aacgcccgca ttattgacaa ggatgtccgc cggccccagc gtctgctcta cctgcgcaaa
     6601 catcgtgcgc accgccgctt cgtcagagac atctgcacag aatactgccg ccgtgccgcc
     6661 cgcagcgcgg atacgagccg ccacgctttc ggcactgtcc cgccgggtgc ggcagcacac
     6721 ggccacacgg cacccctgcg cggccagcgc cagcgctgcg gctgcgccga tgccgcggga
     6781 agctcctgtc acgattgcgg ttttttccat ttcggcttgc tcccatctct gcgcctgcgg
     6841 cgcagctctt caaaaaacgc cgccagcagc gcggcgcatt cgtcctccag cagcccgccc
     6901 tccacctggg ggcggtgatt gaaaggcagc gcaaacagat ccgttacgct gccgcagcag
     6961 cccgcctttg catcccgtgc gccgtacacg acgcgcttga tacggctgtt gacgatggcc
     7021 cctgcgcaca tggggcacgg ctccagcgtc acaaataact cgcattgcca cagccgccag
     7081 ccgcccaaag cgcggcatgc cgcgtctatg gccagcagct cggcgtgatg cgtggcattc
     7141 ttgccggtct cccgcgtgtt gtgtgccacc gccacaggct ccccgtcctt cgccactacc
     7201 gcgcctacag gcacctcgcc caacgcagcg gctttttccg cttcggccag cgccagacgc
     7261 atcaacgtga tatcatccat atgaaaccaa ctttccttat ttcgcgaaca gcggccatgc
     7321 cgcgcgcagc gccatgcacg ccatcaccag cgggaacagc ggcgcagacg ccaggcgctc
     7381 cagacggctc tggcggttct tggggtcgta tacccggggg atgggcctga aacggcgcat
     7441 tacgcccagg cggcgaatgg ccgcaagaca caccacggcc cccacgcaga acacggccac
     7501 caggtatccc gcaaacgcaa aggcggacac gccgtccatc ttctgcgccg catataaaaa
     7561 aagaaacgac attgtattat aagcaaagtg cagcgccatt cccagcgcca gagagccggt
     7621 acagtaggcc gccagcccca tgagcaccga caggatgaac agagacggca tctgcgcgat
     7681 atcgccgtgc agcagcgtga acaccacact ggatacaacg atggaaaacc atgttcccca
     7741 gcgggaaagc actgcctgca tggcgccgcg caccagcagc tcctccagta cggcgggtac
     7801 cacacacatg ccgatgaaat acaaaaacgt ggcaaatccg ctttgtggca gacggatggc
     7861 ctccggcgcg cggtaccggg tgaacgcggc cagcagccgc tgcaaaagcg tagtaagcag
     7921 gctgcataga aagcccgccc ctaaaaacac cggcacgaac agccacagcc gcccgtcccg
     7981 aggcatctgc accgaaatcc gcaggcgcat gggcgtagcg cgcactccca cggccagcag
     8041 ccagaacgct gccgccaacc cggcggcggg cagaagaacg ttgaatacgc ccagcaccca
     8101 ctcaggcaca gcaatggggt tttccagcgt agcacttttg ttgaacaggc gcagcagctg
     8161 ccctacggcc aaacgcagca gcgccgtgac gcccaaatac accgccgccg ccagcgccgt
     8221 caggttcgcc gcatagcgca gcgggtcatt ctgttgtttg cgcgccatcc cgcagccacc
     8281 tccctctgtt tattccgccg gcccgcgcgg ggcccgcgcc gatgatatcc agcttctata
     8341 ttacaatatt ccggcaaaaa aaaccactgn nnnnnnnnnc atcgcgtttt cacagcggaa
     8401 atatttttat gaaggactca ccgggagaag cggcgaatca aaacaaccgc gccggttcag
     8461 gcatatgcac gggaaacgga agcgtaaaca gcctcagagg tatcctcccc ttcgatccac
     8521 ccggtgtagg acacgcggac cgtatcgcct tttttcagct cctccggaaa agactttccc
     8581 ggcacagctt caaatttcag tgtccggccg tcggcggcgg ccagcgtcag tgcggtatcg
     8641 ctcacggcac gcaccgtacc gagcacggcg ctcttattgg catctttccg ggccgtgacc
     8701 tccagtgaca gaagctgtgc ggaacgggtg gattccccgc tgatgcaacc ggaataagtg
     8761 acgcgcacac catctcccgg ctccactccg ccctgctgcc cggtgaggga tttccccgtg
     8821 tagaaggcaa gtacacgtcc gtccgccgta cataccccga tgctgccctg tttgctgttc
     8881 acggaacaca ccgtgccccg cacagcctgt gcatccgcca gcttttccgc ctgcacacgc
     8941 agcagcatgg catgggatgt atcctccggc gcggcgctgc ccgaatagct cagcaccgcg
     9001 cggcttcccg cgcgcaggcc atccggcata tccacctcgg cggagcccag cgcgaagata
     9061 taacgttccc ctgtatctgc caaaaaggtg atcgtatccg ccttttcatc cacggcgcgg
     9121 atacgtcccg ccagggtgaa cacatctccc tccgatacgc gcgctgtcac acggctcacc
     9181 accgcgccgt ccggtgcgcc atcaaaatac acgcgcaccc acattccctc gcgtggttcg
     9241 cccttcagcg cacgtacgct ccggcccgcc gcaaaggtgt aggtttcacc gcccgccgtc
     9301 cgaagtgtga tgctctcttc atcgaacgcc gttacaacgc cgtcggtcgt gctccccatc
     9361 gaaagggagt cgggctgcgt ttcctgcgca gcctctatgc gcagcagctt tacatttcgg
     9421 gcgttggtgt ccgtcaaaac gccgtcgtac cagatgcgca catactggcc ggcctgtacg
     9481 ccgccttcta ccatgtcctc ggcgccgtcc atacggaaca catactctcc gccgcctgcg
     9541 gcacggagga tgaactgtcc gtccgaggcg tccagtacca cgccttcttc ttttttagct
     9601 ccctcgggaa gcaccgttcc cgcctgaggg tcctgtatgg gccgccctcc gccggacccg
     9661 gcgcatgcct gcagccccaa caccagcatc aggcacagcg ctgctgtcag caccgctaca
     9721 catcttttct tcattgctct caaacccaca tctgcaaccc ttcctgccaa acctccacgg
     9781 tccgcggcat actttatcag tgtatgcaaa aacggcacat gatatcccgc cgccgcagta
     9841 tcgctgacat tgtctttcca ttatagtaga cgctcccgca tccggtttca tttcaaaaaa
     9901 aatcttaaaa atctatgaca atcttccaag cacaccggaa cggaccgtcg gaagcatttt
     9961 tgccgcggca caataaaaaa cggccatacg gatcgctccg catggccgtt ttccggtttt
    10021 aaatcactcg gcgtcctcgc cgtccgcttc gctgccggag agcagcgcct tcatcgaaag
    10081 gctgatgcgc tttttctcga agtccacatc ggtgagtttc acttccactt cctggcctac
    10141 gctcagcaca tcggagacct tctcaacacg ctcgttggaa atctcactga tatgcaccaa
    10201 gccgtcgatg cccggcagga tgcgcacaaa tgcgccgaac ttggtgatgg acaccacggg
    10261 cgccttgaac acgctgccga tgggatattc ggccttgaat ttctcccagg gattgtcctc
    10321 ggctttctta tagcccagag acactttttt gttctcggtg tcgatatcct tgacgtacac
    10381 atcgatggta tcgccgacgc tcacgacctc ggacggatgc ttgatgcggt tccaggacag
    10441 ttcgctgata tgaaccaggc cgtccacgcc gccgatatcc acgaacgcac cgtagttggt
    10501 caggctcttg acaacgcccg tgtactgctt gccgatctcc acattggccc agaactcctc
    10561 gcgcttggcg tcgttctcgg cggccaggac ctcgcggatg gaacccacga tgtggcggcc
    10621 ttcgcactgc ttcatcttca gacggacatt cgttttcacc agggcggtat aatcctcgcc
    10681 atggcgcagc gtggcctggg agcgcggcac gaacacgcgc acgttattca ccagcaccac
    10741 aacgccgccg gagttggcct cggtgacgaa gccctccagg atctcgccgc tctcgacagc
    10801 agcggcaacg gcctcgcggc ccttgttctc atccagacgc tttttggaaa gatagacaac
    10861 gccttcctgg tcgtttacct ttgttacgat gaggtcgagc tcgtcacctt tcttgaccag
    10921 atcctccgct ttggcggacg ggtcattggt gagttcctcg agcttgacga aaccggtctg
    10981 cttcgtgccg atatccacct ggatctcgct gggggaaacg ccggtgacga tgccctttac
    11041 gacctttcca ttgtataccg gcttcaagga agcgtcgatc atttcctcaa agctcaattc
    11101 ctcttcacga atttcctcgc tcatactgct cagtacctcc tcaataatag acgagggcgt
    11161 ggatgcaccc gccgtcactc ccaccgtgcc tgcgccgcgc caccacgcgg ggtccagctc
    11221 ctgtgctgtc tccacggcgg cggcctttgt atggcgcccg gcaacggata cgagtttctg
    11281 ggtgttggag gactcccgcc cgccgatgac gaccatcagg tcgcattggc gcgcaaggca
    11341 ctccgcctca tgctgcctcg cccatgttgc attacatatt gtatcaaata tgctcggatt
    11401 tgtatatact tttttgagaa actctacgca ttcttgccac tttttcacct caaaagtggt
    11461 ttgagctacc acaaaaattc catttttggt attttctgga gttaaaagtg cttttaactc
    11521 ctccaaatcg gcaacaacaa atacactgcc ccgtgtgtgt cccacgatgc cctgcacctc
    11581 gggatggttt tcgtcgcctg cgaccagcag ggtcctgccc tcctcacccg ccctgcgggc
    11641 caggcggtgg atcttggcca caaaggggca ggtggcgtca tgcaccacaa ggccccgggc
    11701 cccaagcgca tcgtacacgc actgcggcac gccgtggctg cgcaccacca cctcgtagcc
    11761 gtcgggcacc tgttcgggtg tatccacggt aacggcgccc cgggccgcaa ggtccgccac
    11821 gcattgagga ttatggatga gcggccccag cgtagccacc cgcacgccct gctccagcag
    11881 gccgtaggtg agctttaccg cacggtccac tccgaagcag aagcccgccg tctttgcctt
    11941 aatgatcttc atatcacagc tccttggaaa aagggcgccg caggcagcgc actttctctt
    12001 ttacactttt ataagtattg ccggttttcc tccagcagct tttccagctc ttccgccagc
    12061 aacgccttgc attcgcgcag gcgggcggcg ctgcgcggct cgcccagctt caatttttcc
    12121 gcggggatcg gcttgccgaa cactacggtg cagcggccga acacccgcat ttttccgcct
    12181 ttgtagatga tacggcaggg gatcatatcc acgcccgctt cggccgcaat gacaaatgct
    12241 ccgctcttca gcttacccag gttgccgtcc ttggtacgcg tgccctcggg gaagaccagc
    12301 gccccgcgtc cctcacgcac ctcgcgcaca gtgttttcca ccactgtggc gtcgcccttt
    12361 ccccggcgca cacaaacgac gcccacatgc cgccaggccc agttcagcag gcggctgatc
    12421 tcgaacagct cttctttggc catgatgacc atgggcctgc cccaaaaccg ggcaataacg
    12481 acgaatacag ggtcgatggc ggagatgtgg ttgggcgcga tgacgaagcc cctgtcatgg
    12541 atgaggttct cccttccgat gaccctgatc cggaacgcga tgtgccacac cagccacgcc
    12601 aacggcacga tgatccagta aaaccacatc gttacccctc ctgccccaga cgcgcgcgga
    12661 tggtggacag cagcagcgcg aacgcatcgt cgaaggtaaa gcccgtggtg tccacggtaa
    12721 cggcatccgc agcggggcgc agcggcgcgg cggcacggtg ggtgtcgtta tagtcccgcc
    12781 gttccacatc cgccagcacc tgctcgtagc tcacccattc cccgcgggca atcaattcat
    12841 cgtagcggcg ccgggcgcgg tctcccgcgc tggccgtcag aaatatcttc acctgcgcac
    12901 cgggcagcac cacagtaccg atgtcgcgcc catccataag cacattgtta tcctttgcca
    12961 atgcacgctg ggcgtccagc aggaacgcgc gcaccgacgg gtgtgccgaa acatgggatg
    13021 cagccatgga cacctcctcg gtgcggatga ggccggaaac gtcctccccg ttcaaaaaca
    13081 tatgctgcac accgtccaca tagcgcatct ccagccggat gcccggcagg agcgccgcca
    13141 cggcatcctc atcctcggtg tctgcgccgc tgcgcagcac atgcagcccg atggtacggt
    13201 acatggcgcc ggtatcgaca taaatgtagc caagctccgc ggcaagccgc cgcgacagac
    13261 tggatttgcc cgcaccggac gggccgtcga tggcaatgga aatcatattt ttctcctctt
    13321 cccccccagg acgctccgca ctgcggcatg cgcccggtca tgattgacag ccgtccggcc
    13381 gattacagat ggcttgccgc cgcatatgct gtggcgaacg cgattcccag gttgtagccg
    13441 cccgtgtacg cgtccacatc cagcacctcg cccgcgaaat acagccccgg acacagcttg
    13501 ctctgcattg ttttggggtc cacttccttt acgctgacgc cgccgctggt aacgacggca
    13561 tgggcaaggt cgccctttcc ggccagctcg accgggaagg ctttcagcag ctccaccagg
    13621 cgccggcgct cctcccgagt gatctggttc accttgcgct gcgggtccat gccccacacg
    13681 tccagcatca caggctgcat ggaggccgga agcagcttca caaggcagtt cgcagcctcc
    13741 cggttcgcaa gaagcgcaaa gtcctcggag acgcgcttat aaagcttttc cggcggcagc
    13801 gcaggcttga ggtcaataca gacgcggtag ccgtattttt tcatatcccg gatgtgtgcg
    13861 ctggcggaaa gcaccagcgg cccggacatg ccgaaatgcg tgaacagcat ctcaccctgc
    13921 tcgcaaaaca gcgccttttt tccttcgtac agtgtgagcg ccacattttt aagggaaaga
    13981 cccatcatac gcctgcacac ccctccgcgc tccaccaggg aaacaaggct cggctccggc
    14041 ggcactacgg tgtggcccgc ctgccgtgcg aaacggtagc cgtcgcccgt ggagcccgtc
    14101 accggataag acacgccgcc cgttgccacg agcacggccc cggcacgcag cgcttcgccg
    14161 ctctgcaggc gcacgcccgt gcagcgaccg ttctccaaca tcagctcccg tgcgccctcg
    14221 taccgcagac gcgcaccctt cgcccagcgc agcagagcgt ccagcacgtc ctgcgcgcgg
    14281 tcgctctgag gaaatacccg ccgcccccgc tccgttttaa gcggcacgcc cagccctttt
    14341 tccagcagct ccatcacgaa agagggcggg caggcgttga gcgccgaata caaaaaacgc
    14401 ggattgcggc gcacattttt caaaaactca tccggaccgc agttgttggt gacattgcac
    14461 cgccccttgc ctgtaatgag cagctttttg cccggcgcgt ccatatgctc cagaaccgtg
    14521 acgcgtttgc ccgcgtgcac ggcagcgccg gcggcaaaca gcccggccgc gcctgctccg
    14581 atgatgatac aatcctccat ggcttatcct tcccgcacga cgacagtccg ccgcgcgacc
    14641 ttttcggcat cttttgcatc agggaagccg tccgggacgg cctcagcaaa aacgcggcgg
    14701 agttttgtcc ccgaagccgc gtttgagaaa agcgcaagca cggcagacca aaatatccgc
    14761 gaaatgtgnn nnnnnnnncg ggtttgaggt tattatacca tcaatcccct tgtgcaacaa
    14821 ccgttttccc tcagctcccg cggcagccgg ccgccatatg ccagacatta ttataacggc
    14881 ccgtgcagaa atactcaacc tctcatgctt atttcaccct tcagcacgca catgtgccgc
    14941 ccgtgttgcg cacgcagtac ttatagtgat ataataatat acaattgtgg ccatagccgt
    15001 gtgcggtaca tctgcacggc tgccaagcaa acggcgcccg gccccggcgc gccgcaaaag
    15061 gagacaagcc atgtcgaaac tgaaaaagct gctgccgaag cgggatctgc gcgcacggtg
    15121 gctgtgggta ctggcgctgg ccctcactgc cgtcaagctg ggcctgtgca gcttccagct
    15181 catcgtcgcc tcgccggatc tttcccccat cgacgacacg ctgatgttca accttgccaa
    15241 gagcatctcc gccgggaact ggctgggaga atacgattgg ctcacgttgg gcaagcattc
    15301 gttttatgcg ctctggctgg cgtttttgaa cctgctgcac gtcaacatcg tcgtaggcgg
    15361 tcaggcgttg ttcgcggtga gctgcctggt gctgctggcg gcgctgaagc ccgtgatgcg
    15421 cacaaactgg ggacggctgt tcgtattcgc cgtcacgctg tataccccgg cctcctgggc
    15481 ggaaaacacg ctgcgcgtct accgcgacaa tatctatccc tccctggtgc tgctggcgct
    15541 ggcaggacta ttgggcgcat tcacgcgctt tcgggaaaaa ccgctgcgcg cgctgcctta
    15601 ttatgttgca gccggccttt cccttgcggc ggcctggctg tgccacgagg acaacgcgct
    15661 gctgctgccg ttcgttctgt gcgcggcggc ggtttatctt gcttatctgt ttctggacaa
    15721 aagcattgcg cacaaaaaga gccgcctggc gctgctgctg gtaccattag cgctgtgggg
    15781 cggcggcatc gcggcctggt gcggcatgaa ctacaagtat tatggccgtt ttatcatcag
    15841 cgatttcact tccagcgaat tcaatgacgc tatgggtgcg ctgtcccgcg cgtatccgga
    15901 tgaccagaag cgctacgagc tcgtcccttt gtccacacgc cttgcattgt atgaagtatc
    15961 ccccaccttc gccaaacttc aggatgtgct ggagacgccc gagatgtaca acggctacgg
    16021 cgacccggaa acaggcgagc tgaattccgg cggcatccat tgggtgctgc gcaaggctgc
    16081 ctggacggcc ggttactacg acacagccca ggacgcagaa aacttctggc gcgcggtggc
    16141 cgacgagata aacgccgcct gtgatgcagg gcttgtcccc gccgggcgca gacacagcgg
    16201 tgtgttttct ccgatcaagg cggaatatgt cgcccctacc atcggtaaat tttttgacga
    16261 ggtcaaggtg ttcgttctct tcgagcagac cgaaccgacc cagatacttt ccatcgcccg
    16321 gcccgaccaa actgaagaat gggagagtta tctgcactgc cagagcacca tcgccgcaca
    16381 ggccaacacc gatctgccct actttgcgcc tctcaatcaa attgcctaca agctgctgaa
    16441 tcttgtaacc tgggtgcagc gcatcctgct ttggcccatg ctgctgctca ccgtgctgtg
    16501 gcttgtgtgg tatgcgcccg cttgcgtacg cggactgaaa aaaaagcagc cgcctgcgga
    16561 ccttgcgggc agcgtgctga tgctgggctt cttcctcacc gggcttctgc gcatggctgc
    16621 tattgcctat ctcatggccg tgacgctttc attcgacatc tatctgatgt acctctcggc
    16681 agcctgtgcg cccatgctgg cgtttctggc ttacggcacg gccaaatggt tcgaggggca
    16741 cgccttgtcc gcttccccgt acagccttaa ataagaaagg acgttcttca acatgaaaga
    16801 ctatatcaag atcgcccgtc cggaccactg gatcaaaaac ctgttcatct tccccggcac
    16861 gctgttcgcg ctgctgctgg taggctggaa gggctcctac ggcggcctgg ccggtatcgc
    16921 gctggtcagt ttcttctgca cctgcatgat cgcctcggcc aactatgtca tcaacgaatg
    16981 gctggacgcc cggttcgaca agtaccatcc caccaagaaa aaacgccccg tggtgcagaa
    17041 cactatgagc ggaaaaatcg ttgccg
//
LOCUS       DAWBSB010000019        16892 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_880_length_16892_cov_3.593871, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000019 DAWBSB010000000
VERSION     DAWBSB010000019.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 16892)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 16892)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16892
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_880_length_16892_cov_3.593871"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1914..1923
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3013 a         5464 c         5042 g         3363 t
ORIGIN      
        1 ccttgcggcg catctttaca ctgtcgtggg agggaatgcg gccctttgag gacgtggaga
       61 tcacatagtc caccagcccg ctttcaaaca ggtccatgat attgggccgc gcctcgctga
      121 gcttgcgcac ctcgttgcag gccaccatgt tctggcccag atagtacgcc gtgccggacg
      181 tgccgtacag cttgtacccc atgtccttga gctgccaggc gatgtccacc agctcgcgct
      241 tgtccgcgtc ctttaccgtc aggataacgc atttgcccgg cgtgggctgc cgcaggctgt
      301 agcccgcgcc cacaaggccc ttgagcagcg cgtcctcaaa ggttttgtcg atgcccagca
      361 cctcgccggt ggatttcatt tccggcccca gctgggtgtc cacatcgtgc agcttttcga
      421 agctgaacac cggtaccttt acggcgcagt aatcgcccgt gggatacagg cccgtgccat
      481 agcccatgtc ccgcagcttt tcgcccagca tacagcgcac ggccagctcc accatgggca
      541 cgcccgtcac cttgctgatg tacggcaccg tgcggctgga acgggggttc acctcaatga
      601 tgaacacctc gttgttgtat accacgaact ggatattcat caggcccacc acgttcagcg
      661 cgcgggccag acggcccgta taatccacaa tggtgccctt gatcttctgc ttgaggttct
      721 gcggcggata caccgagatg gagtcgcccg aatggatgcc cgcacgctcg atgtgttcca
      781 tcatacccgg aatgaggaag tcctcgccgt cgcagatggc gtccacctcg cactcggtgc
      841 ccatcaggta tttgtccacc agcaccgggt tcgacatatc cacatggctg gtgatgatgg
      901 ccatgtattc cctcacatcg ttctcattgt aggcgatgat catattctgt ccgcccagca
      961 cgtaggacgg acgcagcagc accgggtagc ccagctgtgc gccggcggcc agtgcctcct
     1021 cacaggtgaa caccgtcgaa acgctcccgg tcctccgcct cgtcgatggc gtccgcactg
     1081 gtgcccagaa tcttcacacc cagctttgtc aggtgttttg tcagcttgat ggccgtctgg
     1141 ccgccgaact gcaccacaca ggcccatggc ttttccgtgg cgatgatatg ctccacgctt
     1201 tcttccgtga gcgggtcgaa atacaaacgg tcgccggtat cgaagtccgt ggagacggtc
     1261 tcggggttgt tgttgacgat gacagcctcg cagccgtggc ttttcagcac ccatgtgcag
     1321 tgcacgctgc aataatcgaa ctcgatgccc tgcccgatgc ggatggggcc gctgccgaac
     1381 accagcacct ttttcctgtc ggaggggtgt tccgcgataa actgcgccgc ttcgttctcc
     1441 tcgtcaaaag tggaatagaa atacggcgtt ttcgcactga actcggccgc gcaggtgtcc
     1501 accatcttat aggacgcgcg cagcttttca cagggcagcg catccagtcc cgtcagacgc
     1561 agcacggtct tgtccaggaa gccctgcatt tttgcggtgc ggtacagctc ttccgtcagt
     1621 atgccgcctc gcagcccggc cagcgcaagc tctgtttcgg ccaacccttg cagctttgcc
     1681 aaaaaccacg ggtcaatttt agtgatattg taaatcgtct caaacggcac gccgcgcttg
     1741 atggcctcgt acacggtgaa ggaacggtcc gagtcgcagc ggtgcagccg ctggacgatc
     1801 tcctcgtcgg gcaggctctc cagctcgggc agggtcaggg tctccaggcc cagctcgatg
     1861 gagctgacag ccttcatcat ggcgtgctcg aagctgctgc cgatggccat gacnnnnnnn
     1921 nnnggccatc acctcgccag tggccatcat ctgcgtgccc agggatttct tggcgtatac
     1981 gaatttgtcg aacggccatt tcgggaactt gaccacacag taatccagcg cgggctcgaa
     2041 gcaggcgcag gtctgccccg tgacgtcgtt ggtgatctcg tccagcgaat agccgatagc
     2101 gatcttcgcg gccaccttgg cgatgggata gcccgtggcc ttgctggcca gcgccgagga
     2161 acgggacacg cgggggttca cctcgatgac ggcgtattcg aagctgtcgg gctttaaggc
     2221 aaactgacag ttgcagccgc cctcaatgcc aagctcggtg atgatattca gcgccgaggt
     2281 gcgcagcatc tggtattcct tgtcggagag cgtctggctg ggcgccacga cgatggaatc
     2341 gccggtgtga acgcccacgg ggtcaaagtt ttccatattg cagacggtaa tgacgttgcc
     2401 ctttccgtcg cgcatgacct cgtattcaat ctccttccag ccggagatgc atttttcaat
     2461 gagcacctga tggatgggcg aaaggcgcag accgttggtg ccgatctcgt gcagctcctc
     2521 cacggtctca cagatgccgc cgcccgtgcc gcccatggtg aacgccgggc gcacgatgac
     2581 cgggtaaccg atctcgcccg cgaacgcaag cgcgtcggac aggctctcca ccaccttgga
     2641 agggataacc ggctcgccca gcttctccat ggtgtccttg aagagctggc ggtcctcggc
     2701 gcggtcgatg gtgtcgggct tcgcgcccag caggcgcacg ccggcctgct ccagataacc
     2761 gctgcgggcc agctccatgg ccaggttcag gcccgtctgg ccgcccaggt tcggcaggat
     2821 ggaatccggc ttttcctttt caatgatgcg cctcagcacc gtcagggtca tcggctccac
     2881 atacacgcgg tccgcgatgt ccgggtccgt cataatggtg gcggggttgg agttgacgag
     2941 caccacctcg atgccctcgc ttttcagcgc gcggcacgcc tgcgtgccgg agtagtcgaa
     3001 ctcggcggcc tggccgatga tgatggggcc gctgccgatg acgagcacct ttttgataga
     3061 cgggtccttt ggcatttcag cagcctcctt tcatcatagc gacaaaatcg tcgaacaggt
     3121 attcggtgtc cctggggccg ccgcaggcct ccgggtggaa ctgcaccgta aagcagggga
     3181 tgtttttata gcggatgccc tcggtggtac cgtcgttggc gtttacatgg ctcacctcgg
     3241 ccacggcggg gtccacggta ccggccacaa cggcatagcc gtggttctgg ctggttatga
     3301 acgtgcggtc cttggcaaaa tccgtcaccg gctggttcac accgcggtgg ccgtatttca
     3361 gcttttccgt ttttgcgccc gtggcaagag ccatgagctg gtggcccaga cagatgccga
     3421 aaacaggccg gcccagcgcc gcgatctcct tcaggttttc aatgatcttc acattttcgg
     3481 ccgggtcgcc cgggccgttg gagagcatca cgccgtccac ctccagcgcc gcaagctcgg
     3541 ccgcggtggt gcgtccgggc accagcgtca ccttgcagcc gcgttttaca agattgcgca
     3601 ggatgttgcg tttatagccg aaatcgaaca gcgcaacatg gtactttccg ttttcgttaa
     3661 atgtttccat acattttgta gtaacgctgt ccaccgcgtc cttcaccgta taggctttga
     3721 tcttttccat caaagcggcg tcggcgcgca cggcgtccgc gctcttatat tcggtggtga
     3781 gcgcgccgtt catcacgccg ctctcgcgga tgatgcgcgt caggcggcgg gtgtccacgc
     3841 ccgcgatgcc gatgatcccc tgctccttca aaaattcgtc cagcgttttt tggcagcgga
     3901 aattggacgg cgtgccgcac aactcccgca cgatgtagcc gaaggcccaa atgcgcgcgg
     3961 attcggcatc ctcgccgttc acgccgtagt tccccaccag cggataggtc tgtgtgatga
     4021 tctggccgta atagctgggg tcggtaaggg tttcctcgta gccgaccatg cccgtgttga
     4081 acacgacctc gcccacactg gtaccggctg cgccgaaggc ccggccttcg aacaccatgc
     4141 cgttcgccag cagcaaatat gctttcatat gcgctccccc ttttttcaag ctccgcgcgc
     4201 tcacagcgtc tgcctggcct gttctttcat tttacggctg cccagatgca ccagcggcag
     4261 cttgccctct ttgcagatgg ggcattcctc cggcgcatag ttggggaggt ccagcttggc
     4321 ggcgctggcc aggatgggaa tgggcgaggg cgcgtccgct ttggtgcggt ccaccacgca
     4381 cacgatgccc aggcattcgc cgccggcctc ctcgatgacg cgcttcgcct ccagagaaga
     4441 cacgcccgtg gtcaccacat cctcggcgat gatgcacttt tcgcccggcc ggatggaaaa
     4501 gcgcttgagc gtcatcacat tgtccacacg ctcggtgaag atagcgcgtt tgcccgtctg
     4561 gcgggccagc tcgtaggcgt aaacgatgcc gcccatggcc ggaccgacga tgacgtccac
     4621 gtccagttcc cgcagctttt cggccacggg cttcatggcc gcggcgcatt tttcggggta
     4681 ctgctgcaga tacgccatct ggcagtaggc cgaggaatgg cggccggagg acagcaggaa
     4741 gtggccctcc agaaacgcgt cgcaggactt caatatttca agcacatcgt tcatgtggat
     4801 tctccttttt attccaatca gatctgtgta ttttcggctt agacctgcgc gcagccgcgg
     4861 atctccgaaa gggagcgcac accgtggctt tgcatccagg cggcgatgcc gtcggtgatc
     4921 ttttcgcagg cgcgcgggtc gctgaaattg gcggtgccca cctgcacggc cgccgcgccg
     4981 gccatgatga actccagcgc gtccccggct gtggcaatgc cgcccagccc caccacgggc
     5041 acctgcacgg ccctgcacac ctgatacacc atgcgcaggg cgatggggcg gatggccggg
     5101 ccggacagcc ctgcaaacac attgttgaat acagggcggc gtttttccag gtcaatggcc
     5161 atggcctgga acgtgttgac gagggaaagc ccgtccgcgc ccgccgcctc gcaggcaagg
     5221 gctatttcgg tgatgttctc cgcgttggga gagagcttca cgataagggg ctttttcgtg
     5281 caggcccgca cgcgggacac tacttcccgc gcgctttccg ctttcacgcc ataggcgatt
     5341 ccgcccgcct tcacgttggg gcagctgatg ttcagttcga tgaagtccac attcgtttca
     5401 tccagcagcc gcgcgccctc ctcgtaagtc tcgatggtgc tgccgcccag attcgccaac
     5461 accgcggggc caagcttttt catgccgggc agctcatact caatgaaatg ccgcacgccg
     5521 ggattctgca gaccgatgga gttgatgagc ccggcaggtg tctcccacag acgctcgccc
     5581 tcgtttccgt acttgccctc cagcgtcagc cccttgccgg agataccgcc cagctttttc
     5641 atgtccacga tgcccgcgta ctcgccgccg aagccgaagg taccggacgc ggcgatgacc
     5701 gggctgcaca gcgttttgcc gcacagggta acgtcagtca agctcataca gcacgctccc
     5761 ttcaaacacc ggcccatcct tgcagacaga cacgccgccg tttttgctct tgcaggtgca
     5821 gcccagacac gcgcccacgc cgcaggccat cttggcctcc ctgctcacat atacgcgcac
     5881 gcccgcacgc agacacattt tggccacctt ttccatcatg atctccggcc cgcaggtaca
     5941 cacggcggtg tacttcgcag ggtccagcag gtccgtcaca aagccgtggt ggccgtggcg
     6001 gccggaatcc gtggcgatgt gtaccgaacg gcacgcagcg ctgaacgcgt caacacggta
     6061 cggctcgtcc cgaaagcccg cataaaggtc cacgtccacg ccctgcgcgg caagctctgc
     6121 cgcaagctgc agcagcggcg cggtgccgat gccgccgccc accagcgcga cggcgccgcc
     6181 caacgtgccg tccacgggaa acccacggcc cgaggggccc gtcagcgcaa cggtgtcccc
     6241 gggccgcagc gcggccagct tctgcgtgcc cgtaccacgg acctcatata aaaaggaaat
     6301 ctcttttttc tctctgtcaa aacgatgcac actgatgggg cgcgaaagca gcggcgcttc
     6361 ctccgctttc caggcgcgca gcatgtaaaa ctggccgcac gcgggcgcgg gggtggtttc
     6421 gtcccagcgg gccgtcagca gcatcacgtt ttcgcccgcc gcgccgtttg ccaggacggc
     6481 ggcgttataa tttgctgcag gcatccgcaa tggcctcctt catatgcacg cattcgttgt
     6541 acgcggcttc ggcataagcc gttccgggct gttttttata ggccagcagc acgctgcggc
     6601 tggagttcac cacaccgccg ttgccctgcg ccagatactg cgcgatgtcc tcggccgtgc
     6661 cgccctgcgc gccgtagccc gggatgagga aaaagctgtc cctgtatttg gcgcggatct
     6721 ctttcgcctc ttcgatgtgt gtgccgccga tgacgagccc cagcgaggaa tagccgtgcg
     6781 cgcccatgta gtccttgccc attgcgctga gcttgtcgcc caccaggtca taaacgtggc
     6841 ggccgtcggc cagcttttcg tattcataat ctttcgcgcc ggggttggag gtgcggcaca
     6901 gcacgaacac gcccttgccc ttttccgtca tgtatttcgt gtacggcgtg atggaatcca
     6961 gccccatata gggcgccagc gtaacgaagt ccgcctcgaa ctcgccgtcc agatgggcct
     7021 tggcatacat ctcggccgtt ttggcaatgt cgccgcgctt gatgtccgcg atggccacaa
     7081 ggccctcccg gcgcaggtat tccagcgtgg atttataggc cagcatgccg tccaggccca
     7141 gcgcctcgta ataggcgatc tgcaccttga agcagcccgc cacgctccgc gtggcttcga
     7201 tgatatcccg gttgaagcgg aagatgttct cgcccttgga gagagtcttg tccacacact
     7261 ccgcggggat gtaggaaaaa tcggtgtcca gccccacgca aacggggctt ttttcggcca
     7321 cggcctcata caaacggtct acttgcagca taacgtcctc ctgttgtgtg tttatcctat
     7381 tgcttccatt gtcccatagg tgacgcggcc cgccttcacg gtgcacgcca cgcggccgta
     7441 cagcgaaagg ccgtcgtatg gggtgttttt gctcttgccc gcaaaatcct cccggcgcac
     7501 ggtgtaaggc tcgcccagct ccaccagcac cacgtcgccg tcgtatcctt cggcaatcag
     7561 ccccttgttc aggcccagta tctcagccgg gccgcggctc atcaaacggc tcaggtctgc
     7621 cagcggcatc ccgtattccc ggcacaattt ggtgtagcag acgctgaacg ctgtctccag
     7681 ccccaccatg cccggcgcgc cgccgcgctt atcctcctcg gtgtgcgggg catggtcggt
     7741 cgcgatggct tccacctcgc cccgcagcat ggccgcgatg agcgcttcca catcctcttt
     7801 tgcccgaatg ggcgggttca cgcggaaatc gctgtcccaa aaccagatgt ggtggggcgt
     7861 cacctcgcag gtgacgcgca caccccgggc cttggcggca aggatatcgg ccagcgcctc
     7921 tttggtggaa acgtggcaca tgtgcagccg tgcgcccgta gactgggcca gatacaggtt
     7981 gcgagccgtc tccaggtttt ccgccagacg gtaatccagc gcggaaaggt ccatatcctc
     8041 ggcgtggctc atcaccgtca ggcctttttc ggcggcgatc cgcatggcct catacattac
     8101 cctgctctcg cgcacgcctt taccgtcctc gctgatgaag cggatgttcc gcggcagcgc
     8161 ccgcagatgg ccggtggtct tgccgtcgaa gtctttcgtg atggacacac actggttcac
     8221 gtcgcacagg ccgatgcgcc tggcgtcgtc catgatgctc tgggcaatgg ccgcggaaga
     8281 acacacaggc cttgtgttcg ccatgcagtt gacgaacgtg taaccgcccc gggcagccgc
     8341 ccggctgccg gagtcgatat cctccttata ctcaaagccc ggcgtacgga agtggcagtg
     8401 caggtcaatg aaagcaggca gcagcgtgcg gccgcctgcg tccagcaccg tatccccgcc
     8461 ggcggaaagg ccctcgccca cggccgtgat gacgccgttt tcaatgcgca catcgcccgg
     8521 acgcgcaccg tccgcatccg tgagcacggc attgcaaatc agcataagcc attctccttt
     8581 ctgttcctca aatacagcaa aacggcaaaa aaaaactttt cccaaaacgg gaaaagttta
     8641 aaaagccggg aatagaacgg cgggaaaaga aaacaaagcg ccctgcacga tgcgaaaacc
     8701 cacgttcagc cccggcatgc atgcgttccc ccgttcccaa ttatttctac ttattctatc
     8761 attctgtaaa gagattgtca attgaacggg tatgcagaag atagcgccct gtaacggggt
     8821 ttttctgtgt gtaatccaca aacaccacct gccgtgcgcc gtacaaaagc gccacgccgc
     8881 gccgcacgcc gggcaggggc gcagtggcag ggttttgtgc cagccgcttc accgcgcggc
     8941 ccagcaaagc gtccgttaca tgcaggcatt gcggcaagcg ccctgtggaa tttgtggcaa
     9001 ggcggtcgcg caccatcgcg tcccgcaggc aggctttgtc tatgcccggc agcgccgcgc
     9061 accagttgta caaaaaagcg gtgtaatcgt ccagcggaat attcgccgtg ccgtgccgcg
     9121 cgcctgccgc gcccaaacgg cagtaaaaaa cgaacgggga aagccctgcc gccgccatcg
     9181 cgtaagccgc cgtttccaaa aaacgcccgc tgttgtagca gcgctccagc gcgtcttcgg
     9241 cggcgcgcag cacgtccagc tcctccggca aaagccaggg cgttttctgt acttcatacg
     9301 gcgggtcttc cgaaaaaaca caggggaatt cctccggctc ctcccgcatg gccgcgccgt
     9361 gcagcagctt cagaaaaccc agctgcagca tctgtgcgcc cagcgcatag ccggtgttga
     9421 agctttcacc gaatgtccgc agatcctcat ggggcaggcc cgcgatcagg tcgatgtgca
     9481 cgtgcatatt ccccattgca accaaacgcc ggatgttctg ctttaaaacg cccgtatctg
     9541 ttttgcgccg cacggcggcc agcgtttttt cgcaaaacga ctgcatgccg atctccagct
     9601 gaaacgcgcc cggcggcgcc tgctccagca gcgcgaaggt atcttcccgc aggatatcgc
     9661 ccgcaagctc gaaatgaaag cacacgcccg ccggaatgtc ccgcccgtaa tgcttcagga
     9721 taaacgccaa aatggcattt gcgtgggcgg ggtttgcatt gaatgtccgg tcgatgaatt
     9781 tcacggtgtg cgcgccggag cgcgccagcg tcaggatgtt ccgttttgcc tgctccagcg
     9841 ggaaccagcg gggcttgccg caccgcccag aaaggcagaa cgcgcaggca tacgggcagc
     9901 cccggctggt ctccagatac gcaatgcgcc cgcgcaggtt ctccagatac gccggcgcat
     9961 acgggctggg cggcgtgccc tcctccacgc agggcgcgga gagaaagccc cgggcgcaca
    10021 gccccgggat gggcggcgcc gcctcctccg gcttccgccc ggcggccagc gacgccagca
    10081 gcatgggcac gctgcgttcg ccctcgccgg agaggatata atccacctgc gggttttccg
    10141 ccaaaacctc ccccgcgttg tagctcacct ccgggccgcc cagcactaca tatgcgcccg
    10201 gccgcgcagc ctttacagcg gcggcaagac gcagcgtttc ccgaatgttc cacaaatagc
    10261 acgaaaacag caccgcatcc ggctccccgt ccaaaatgcg ggccagcacg tcccgctccg
    10321 gctcattgac ggtgccctcc cgcacctcgg cacaaacacc cgcagggcct gcggcctcca
    10381 cacccgccag caggcaccag ggcgccagcg acgaatgaat gtatttcgca ttgagtgcaa
    10441 acagcaccgc cttcatcggc cgcgcccccc tctttcaccg tttttacgct tgcaaataaa
    10501 aaacgccgcg tcacgggcgc gttgacacag aaaacatcgg ttttcttgcg tcagcacgcc
    10561 taaagcggcg ttttgctttc ccgcagaaca tggaacatgc gcacgagcag cagcaggaac
    10621 agggctgcca gcagcccaaa cacgacgccg gaaaaatcga tcaccacatc ccgcacgtcg
    10681 ctgccgcgcc catccgcaaa tatctggatc ccctcgtcca gcacgcctgc cagcaggccg
    10741 aaaaacagag gccacgaaat atacgagagg atgcgccggg tatacacccg cagggacagc
    10801 atcagccaga agcccagcaa cgcgtattcg ctgaaatggc ccagcttgcg caccagacgc
    10861 tcggaaaagc gccagccgac ccccacgcgg gccagccctc cgttgagcca ttgggtgacg
    10921 gcggcgctgc gcaggcccga ggccgcgccg ctctgcatgg aattggaaaa aataaacacg
    10981 acagtgcccg ccgcaaacag cgtgaacagc acccgcagca cgatcaggcg tgccggcgtg
    11041 cgctctctgt ccgtcagacg tccgtttgtg tggttttgct gcatcataga taaccctttc
    11101 cgggcaaaca cctttgcgtg gctgtcaaag aagctgcaca ccggctgcgg cgcacgcggc
    11161 gcgcgtttca ccgtcccaca gcgacacctg cacctcgcca acgtgggctt tgcccaacag
    11221 cagcatgcac aggcggctct ggccgatgcc gccgccgatg gtcagcggca actcatccgt
    11281 taacagcatt ttgtggaacg gcagcgccgc gcgttccccg catcctgcga tgctgagctg
    11341 gcgtgccagc gcgtccgcgt ctacgcggat gcccatgctg gacagctcca gcgcacagcc
    11401 cagggtttcg ttccagaaca gcaggtcgcc gttcagcgtc cagtcgtcat agtccggcgc
    11461 gcggccgtcg tgaggcctgc cggaacgcag cgccccgccg atgccgatga tgaacaccgt
    11521 gccgtgcgcc tttgcaaaag cattttccct ttcctttggt gtgagcgcgg gccattggtc
    11581 ctccagctcc tgcgcggtgg caaacgccgt ttcccggcac agccgcacgc gctccagcgc
    11641 ggggaactgc catttcagct catccagcgt gccgcacacc gcatccacga tcttctgcac
    11701 agtctgcttc agatattcta tggttcgctc ctcccgggtg atgacctttt cccagtccca
    11761 ctggtccaca taaatggagt gaaggttgtc catctcctcg tcccggcgga tggcgttcat
    11821 gtctgtgacc aaaccgttgc ccacatggaa atcatactga gccagagcca tgcgcttcca
    11881 cttcgccagc gaatgcacca cctcggcgtt tgcccccagc gcgggcacgt cgaaggtgac
    11941 ggggcgctct acgccgttca aatcatcgtt caggcccgtg gccgggtcca cgaacagcgg
    12001 cgccgtcacg cgcttcagat gcagcgcgcc gcacagcttt tccaggaaga tctgcttgat
    12061 aaggccgatg gccttctgtg tgtcataaag ccccagcgcc ggcctgtacc cggcgggaat
    12121 gacggttttc ggcatgatag aactctcccc cgcgctcaaa atcatacggt ccgttccgta
    12181 tttaatgtat tatagcacgg gcgggggtgt ggttcaagcc accggacggc ttgccacagc
    12241 cgtgcccgcg tgttaccata aggaaagaac gcgccggccc tgtcctgggc cgcgcaaaaa
    12301 aggagcgtgc cgttatggcg gagcaccgca ttttcaaagc ccgtttttcc gatatctacc
    12361 cgctctacgt acagaaggcg ttgcgcaagg gccgcacgca ggatgaagta gacgccgtca
    12421 ttctctggct gacgggctac acgcccggcg gccttgcccg gcagcttgcg cagcgaacgg
    12481 atatggcgtc ctttttcggg caggcgccgc ggttcaaccc agccggcgca gagatcaaag
    12541 gcgtggtgtg cggcgtgcgg gtggaggaca ttgaagaccc tttgatgcaa aagatccgtt
    12601 atctggataa gctcatcgac gaactggcca agggcaggcc catggaaaaa atactgcgcg
    12661 gcacaaaggg cggtggggag caatgctgaa tttcgcgatc ctttcggcgg gaaacattgc
    12721 cgggcgcatg gcgcgcacgc tggacagtct gcgggaggag gtgcagcctt acgccattgc
    12781 ggcccgggac agcgcgcggg cccaagccct ggccgggcgg tacggctttg caaaaagctt
    12841 tgccggatat gaggcgctgc tggccgaccc ggccgtggac gtcgtttaca tcggcagcat
    12901 ccatcctctg cacgcacagc acatccgcgc ggcgctgctt gcgggcaaac atgtgctgtg
    12961 cgaaaagccc ctgacgctga acgcggccca ggcgcaaagc ctgtttgccc tggcgcggga
    13021 gcgcggcctg ctgctggcgg aagccatgtg gacgcgtacg ctgccttttg cgcaggctct
    13081 gcgcaaaacg ctggcggacg ggcggctggg cgcgccgcgc ctggtgacgg ctgtgctggg
    13141 tgaggccatg gcggatatcc cccgcatcgc gcagcccgcg ctgggcggcg gcgcgctgct
    13201 ggacataggc gtttacgcgc ttacgctggc atgcctggca ctggagggag acgtggagcg
    13261 ggttgggggc gtgtgcgttc aaaacgggct gggcgcggac attgaggaga gtatttctct
    13321 gcgtttttcc ggcggagcgt gcgcctcgct ggccgtgagc gcccggtgtg cggcgcccca
    13381 ccgcgccgcc gtttgctgcg gcggaggacg catagagatc gacggcccgc tttggagccc
    13441 cacggccatg acggtgtatg gcggaacggg aacggacgcg ccgcgggaaa ccatccgctt
    13501 tgacaacaca ggcacgggct ttgagccgga ggtgcgggcc gtggcggcgg cgctggcggc
    13561 cggccgcacc gaatgcccgg ccctgcccgc tgccgaaacg ctgcgcgtgc tgcgcgcggc
    13621 ggacgcgctg cgggcgctgt ggggcgtgcg gtatcccggc gaagcgtgag gcatagccgg
    13681 ctctttccgg cttttctttt ttaaaataat tgtaaagccg ggcgcgcgct cccttggcag
    13741 gacggcgtcc ggcgtgctac aatgaaagca acaacaacaa agggggcttc tcccgatgaa
    13801 gctgaattac aaacgcacct ttttcgtggg ccttgcattc atgtccatct gcgcgttctg
    13861 gcaggtgtac gacaacgtca tccccctgat cctgaaagac acgttccatg tgcgcgacag
    13921 ctacatcggc atcgtgatgg ccatggacaa cgtgctggcg ctgttcatgc tgccgctgtt
    13981 cggcaagctg agcgacaaga cccgcacgaa aatcggccgc cgtatgccgt acatactggg
    14041 cggcaccatc ctggcgcttc tgtgcctcgc catcatgccc gcagcacaga agcagaacaa
    14101 cgccctgctg ttcttcatcg gcctggggct ggtgctcatt gccatggcca cctaccgcag
    14161 ccccgccgtg gccctgatgc cggacgtcac acccaagccc ctgcgcagcc agggcaacgc
    14221 cgtcatcaac ctgatgggcg ccgtgggcgg catgctgatg ctgctgacga tgatgatctt
    14281 tcccgtcacc ggggatacgc cccgctattc cgccgtattc cttgtgctgg ggctggtgat
    14341 ggccgtctgt gtggccgtgc tgttctggca gatcagggaa ccggcctgcg tggctgaacg
    14401 cgaggccgcc gacagggccg ccggctcttc cgacgaggac gaaggcgtaa cggacgcggg
    14461 cggcaaaatg ccgcgcccca tgttccgcag cctgctgttc atcctggcca gcgtgttctt
    14521 ctggttcttc ggctacaacg ccgtcacctc ggctttcagc cgttacgccg aacaggagct
    14581 gcagggcaac tttgccctgg tgctgatgat ctgcaccgtc gcggccatcg tctcgtatat
    14641 gcccgtgggc atgatcgccc agcgcatcgg ccgcaagcgc acgatcctca tcggcatcgt
    14701 gatgctgggc acggcgttct tcgcgggtat ctttttccac agcgtctctc cgctgctgta
    14761 catctttttc gcgctggccg gcatgggctg ggcgttcatc aatgtaaaca gctaccccat
    14821 ggtggtggag ctgagcaagg gctcggacgt gggcaagtac acaggctatt attacactgt
    14881 gtccatgacg gcgcagatct ttacgcccat cctctccaat attctgatgg agcatgtggg
    14941 ctatcgcacg ctgtttccct atgccgcctt cttcgtgttc gccagctttg tcacgatgct
    15001 gttcgtgcgg cacggtgatg cgaggccggt tgccaaaaag gggctggaag cgctggacgt
    15061 ggacgactga gcgtgttttt ccctgctcca atgcaaaacg gccgccggaa aggcctttcc
    15121 ggcggccgtt ttttgcggct tttttgaaaa acctctttcc tcggagaaaa aaatcggcta
    15181 tgatagagtg cggtatgcgg cgcagagccg caaaacgacg ggaggatgca gcgatgagag
    15241 cgggaaacga ccttggcagc ggaaggatcg gcgtgctggt aggacgcatc gcgctgccca
    15301 gcatgctggc gcagttcatc tctgtgcttt acagcatcgt ggaccgcatt ttcatcggcc
    15361 agatccccgg cgagggcgcg ctggcgctgg caggcgccgg cgtatgcggc ccggtggtga
    15421 cgatggtcgg ctcggtggcg tttcttgtgg gcgttggcgg cgcgccgctg ctcagcatca
    15481 agcgcggcga gggcgacgac gatgctgcgc ggggcattct ggccaactgc tttttgatgc
    15541 tgtgcgtgtt ctcggtggcg ctgcccgccg ccatcctgcc ctttcgtgag ccgatgctgc
    15601 ggctgttcgg cgcaagcgac gccacctact cgtatgcgga ggcctatttc accctttacc
    15661 tgctgggtac gccctttgcg ctgctggcct ccggcctgaa ccagttcatc gtatgccagg
    15721 gcttcgcggc cgacggcatg aaatccgtgg tgctgggcgc ggtgctcaac atcgtgctgg
    15781 accctgtgtt catcttcgcc ttcggcctcg gcgtgcgcgg cgcggccatc gccacggtgc
    15841 tttcccagct cgcaagctgc gtttatgcgg tgcgctttct gctgggcaaa aagcccccca
    15901 tccgcatcac acggggcgga tattccgcgg ccgtcatgag gcgtgtgctg acgctgggct
    15961 tttcgccgtt catcatcatc gcgctggaca acgtgatgat cattgccatg aacgccgtgc
    16021 ttcagcacta cggcggcgcg gcgcggggcg acgctctggt gacctgcgcc accatcgccc
    16081 aaagcttcat gctcgttgtg acgatgccgc tgggaggcat caccgggggc acgcagtcta
    16141 ttttaagcta taactacggc gcgtaccgca ccgagcgcgt acggcaggcg cagaagtaca
    16201 ttttcgggct ggggctggtg ttcacggctg tgatgactct ggccgcgcgg ctggccgggc
    16261 cgttgttcgt gcggctgttc accaccgacg aagcgctggc ggcccaggcg ttcgacacca
    16321 tcaaggtatg cacgctggcg ctgcttcccc tgggcctgca atacgaggta gtggacggct
    16381 tcacggcact gggccttgcg aagctggcgc tgccgctttc gctgtggcgc aaggccgtct
    16441 acttcgcggc ggtgttcgcg ctgcccgcgt ttttcggcgc ggacgcggtg ttctacgccg
    16501 agcccatctc cgatgtgctt gggccgctgg cctccgtcgc cgtgtactgc gtgtgcatcc
    16561 ggggcgtact ggcccggcgc gcggcgctgg caccgcagaa gcttcccaac tgaaaccgca
    16621 aaaaccatgg ccgcagcgtc tgctgcagcc acggtcagct tgtcgaaaaa gctgcgcttt
    16681 tgcgccaagc tgcctgcgaa cagggcctgc ggccccgttc gccgcgcacg gcggccttcg
    16741 gcggccgctt cgctctgcac gcctcggcac tggccctgcg gggcgccgcg gggttatttt
    16801 agggcaacac cgcacaattc taagtttcga atcgcgccgg cagatttttt ggcaggttaa
    16861 acaagaatgc tcgcgaatac tttgtgtatt cg
//
LOCUS       DAWBSB010000020        16696 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_891_length_16696_cov_3.577670, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000020 DAWBSB010000000
VERSION     DAWBSB010000020.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 16696)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 16696)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16696
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_891_length_16696_cov_3.577670"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3227 a         5030 c         5161 g         3278 t
ORIGIN      
        1 gtgtacaatg tgggcggcca taacgagcgt ccgaacatct ttatcgtgaa gaagatcatt
       61 gagatcctgc acgataaagt agacccgtcc atcgacgaat cgctcatcaa atatgtggag
      121 gaccgcatgg gccacgaccg ccgctacggc atcgacccgg aaaagatcaa gaacgagctg
      181 ggctggtacc ccgagacgac gttcgaggtg ggaatcgaaa agaccatcaa ctggtatctg
      241 gagcacgagg actggatgaa aaacgtgacc agcggcgatt accagaaata ttatgccgag
      301 atgtacaaaa acaaatagaa cggaatgcaa aagcgccgcc cccacggggg cggcgcttgt
      361 tttttgctgt ttgtccggtg gctgggcggc gttctcaata cgccttgccc cacaccacca
      421 tggccttcgc gggcttgccg cagcaggggc atacgtcgga aagatgctcc tgcgcgaaag
      481 gcatgcaccg gctggaaagc cccgcctttt ccttcatcat ttcctcgcag gccagatcgc
      541 cgcaccacat ggtcttgatg tagccgttct cgcctccggc gcgctccagc acctcgtcca
      601 tggtcgtggc cgtccaggtg cgcccggcgc ggtttgcctc ggcgcgggcg tacaggcttt
      661 tgcccagcgc gtccagcaga gcggggatgg cggcctccag gtcttccagc tttgtgaagg
      721 ccttttcccg gttgtcacgg cgcaccagca cgcattggcc gttctcgatg tccttgggcc
      781 cgatctccag gcgcaaaggc acgcctttca tctcccactg ggcaaatttc cagcctgggc
      841 tgttttcgct gtcgtccagc ttcacacgca cgaatttaga gagccgcgcg cgcagctcct
      901 cggccttttc cagcacgccg ggcttgtggg cggccaccgg tacgatgacc acctggtacg
      961 gcgcaatggc cggaggcagg atgagcccct cgtcgtcgcc atgagtcatg atgatggcgc
     1021 cgatgaggcg cgtgctcata ccccagcttg tctggaaggg atactgcagc gtattgtcgc
     1081 gtcccgtaaa ctgcacgccg aacgcgcggg agaacccgtc gccaaagtaa tggctggtgc
     1141 cgctctgcag cgccttgccg tcgtgcatca tggcctcgat ggtataggtg gcttccgcgc
     1201 ccgcaaattt ttccttgtcc gtctttttgc ccttgagcac gggaatgttc agctcgtttt
     1261 cacaaaaatc agcgtaggtc tccagctgct gctcggtctc ctcgatggcc tcctgcgcgg
     1321 tttcgtggat ggtatggccc tcctgccacc agaattcccg cgtgcgcagg aaggggcgcg
     1381 tggttttttc ccagcgcacc acgctgcacc actggttgta tttcatgggc aggtcccgat
     1441 aggagtgcag cacgtgggcg aaatggtcgc agaacagcgt ctcgctggtg gggcgcacgc
     1501 agaggcggtc ctccagcggc tcggagccgc ccatcgtcac ccaggccacc tcgggggcaa
     1561 agccctccac atggtctttt tccttttgca gcaggctttc ggggatgaac agcggcatgg
     1621 ctacgttctc gtgccctgtg gccttaaagc gcgcgtccag cagatgcatg atgttttccc
     1681 agatggcata gccgtaaggc cgcagaatga cgcagccctt gacggtggaa taatccacca
     1741 gctctgcttt caggcagatg tcggtatacc attgggtgaa atcttcgttg atgggggtga
     1801 tggcctccac catttttttg ttctggctca ttgttttgac tcctcttctt ttccgttcgc
     1861 gccacaggca cacaaaaaat ccccctgctg tgcgcatggg ggatcgtcgt caggcagatg
     1921 ctcaggaatt ttcttcgata tagcggacaa cgtcaccgac cgtgcggaag ttctcgatct
     1981 ggtcgtccgg cacttccagg ccgaattcgt cctcaatgga catcacgaga tcgacaacgt
     2041 ccaggctgtc cgcctcgaaa tcttccatga tatcggagtc catggtgact ttgtcttcct
     2101 ccaaatcaag ctggtcgcag atgatctcgc ggatgcgctc aaaaaccata actaaattcc
     2161 ctccaaaata tctctgcgcg cggcgcgcaa gaggccgcgc aaaggtctta taaactttat
     2221 atcgtgtttt acagaggatg tcaagcgtat tcgcaaaata tttttgcgcg gcatttcata
     2281 tgcgcgaaaa cagcaaaaac cgccgcccga aacagtgctt ttgttttgtt gaacatctgc
     2341 aaaaaggacg gaacatgccg cgctttatgc cgccccgctg aacagcggct gcagaaaact
     2401 ccacagcaca ggtaccagca gcgccggcag catattggcg gtcttgatct tcgtcatgcc
     2461 gagaaagttg atgccgatgc acagcaccag gcagtagccc acgccgcaga tctgccccag
     2521 caattcaccc gcgaggaatg gttccagcgc acccgccaga agggaaatgg ctccctggta
     2581 taccagaaca ggcagcgcgg caaagatgac gcccgggccc agcgttgcgc ccagcaccac
     2641 agaggagatg ccgtccagca ggcttttgat gtacagcacc gaggcgtcgc cccgcagccc
     2701 gtcgttcagc gcgccgatga tggccatcgc gcccacgcag tagatgaggg cgccgttcac
     2761 aaagctttgg gcaaagcccg aaaggtgcag cctgcgctcc acaaggccgg acaagccgtt
     2821 cagcctgtcg tcgatgcgca gcgcctcgcc cgccagcgtg cccagcacca ggctgatgat
     2881 gagcagcagc tcgcccgagg aggccagcgt accgtccgcg ttcaccgtga acatggagga
     2941 gattacgccg ttgagcccca gcaccagcac cgcaacgcca agcgccttgt tgatggagtc
     3001 ctgatggctg ccctttaccc cttttttcag cagcagccca acgcagcctc ccgccgcgat
     3061 ggccgcggcg tttaccagcg tgcctgtcat tttttccgtc cccccgcatt tcttttatcc
     3121 ggacggcact gtgcccgctt acagcgaatg catgcgcgcc gccaccggaa tgccttcgga
     3181 tggttccgcg tttggcccca tacagtgccg ctttttcgtt tcagttcagt gccgtgtcgc
     3241 cggagaacag ctcctcggcc agacgtcgca gccccgcgtt tccggggctg cgcagcgccg
     3301 ggtccgctcc gacgagcgcc agcgcctcct tctgggctgc atacaatgca cggctgtcgg
     3361 ccatcaggtc cgcgatgcgc agcgtgggca ggccgtgctg ccggctgccg aaaaagtcgc
     3421 ccggcccgcg tgtttccaga tcgaattttg cgatttcgaa gccatcgttc gtatggcata
     3481 aaaacgagag ccgctttttt accgcatcgg tcgcatggtc gctgatgagc acacagtacg
     3541 cttcgccttt gccgcgtccc acgcgcccgc gcagctggtg cagtgccgaa aggccgtacc
     3601 gctcggcgtt ttcgatcacg atgatattgg cgttgggcac gtccacgccc acctcgatga
     3661 ccgtggtgga caccagcacg tccagcgcac cggccttaaa ggcgtccatc acggcggcct
     3721 tttccgcggc cttcagccgc ccatgcatca gtcccacgcg gtaatgctcc aaaaggggct
     3781 gggctacatc cgtgtaatag gtggtgacgg cctgcatgtc gttttctccc tcgtcgatga
     3841 cggggcacac gatgtatgcc tgccgcccgg cggccagctg cctgcgcaca aagccgtaca
     3901 tgtccgcgcg ctttttgccc gtgatggcat aggtcttgac gggtgtgcgc ccgggcggca
     3961 gttcatccag caccgagatg tccaggtctc cgaacatcag aagccccagc gtgcggggaa
     4021 tgggcgtggc gctcatcacc agcaggtggg gatggtcggc cttggcggcc agcaggctgc
     4081 gctgccgcac gccgaagcgg tgctgctcgt ccgtcaccgc aaagcccagc cgcgcgaatt
     4141 ccacgcccgc gctcagcaca gcgtgagtac ctaccaccag ttccacttca cccgcggcaa
     4201 tggcccgcag cgcggccttt ttcgccgcgc ccttcacgct gcccgtcagc agcgccacac
     4261 ggatgcccag cggcgccagc aggcggtcca gcgtgtccgc gtgctgtgcc gctaaaatct
     4321 cggtgggggc catgagcacg ctctggtagc cgctctgcgc ggccgcgtaa acgcccgccg
     4381 ccgccaccag cgtttttccg ctgcccacgt cgccctgtaa aagccggttc atgggagtct
     4441 ttttccccaa atcggctaaa atctcctgtg ctgcgcgccg ctgtgcgccg gtgggcgcga
     4501 agggcagtgc gttccaaaaa ggggacaggt ccgccgggcg catgggcgcg cccgtgtgtg
     4561 cggcctcccg tccgcgcagc agcagcaggc ccagctgcag cgccagaagc tcctcgaaga
     4621 tcagccgccg ccgcgcagcc tgcacgtcct gccggctctg ggggaagtga atcttctgca
     4681 aggcctcggc cttgccgcac aggcggtatt tttcgatcag cgcggcgggc agcggttctt
     4741 cgatatccgg tgcggcggca aatgcagcac gcacacactt ggccaggtac gcgctgctca
     4801 cgccctctgt ctgcggatac acaggcacca gtggcgaggc cgcggcgctc tctaccgtca
     4861 gcattacggg attcaccatc tcccgtgccg tgaatgtgcc gcccactttg ccgaagaaca
     4921 gatattcgcg cccgacttcc agcttttgaa gcgcatacgg attgttgaaa taggtcaaaa
     4981 ttaaaccggc tgtatcatct ccggccgttg cctttgccat ctggcggccg ccgcggatgc
     5041 gcgcgccgcc gtttttgccg tatacggtgg cgcgcaccac acacttcacg tcataggggg
     5101 cgccggcaac agggtagggg tgggagtagt ccacataatc ccgggggtaa aagcccagaa
     5161 ggtcgtccac agtgcggatg cccagcctgg tgagctgagc ggcacgtttt tccccaacgc
     5221 cttttaaata gcggatatcc aagcctgcgc cctccttttc tgcggtatgc acgtggatac
     5281 cgggtacgcg gcattcacgg cagtgagcag tgaataaaaa aagcgccgcc gattccggcg
     5341 gcgcttatat ttctggtcat atcctgttgc taagcgcaca gtcccacgta ctgtcccgca
     5401 accattcggc gggcacagca atcccatgca cgcggatgca tcagcgcgcg gccgcgccgg
     5461 cggccggaag gttattattc cacgcttatg atatagtaat acaccggctg gccgccgttc
     5521 aggtgtgtga cttcgatgcc tgccgggcat ttcgcctgta ccagctcagt ggtgcgggcg
     5581 gcgtcctcct cgctgacatc gcagccggag atgacggtga tgaaattcgt atcttttttg
     5641 cacatgctgc gcgccaggcg gtacgtcacc ttggtgacgt cggtgcccgt ggccaccagc
     5701 ttaccgttct ccagcgccag gatctcgcct tttttgatct tgcggccgtc gaaatcgctg
     5761 tcgcgggcag caaaggtgac aaggcctgtt gcaactttgt ccgccgcctg catcatgttg
     5821 gtggcgtttt cgtactcgga ggcgtccggg tcaaagttga gcatagctgt gatgccctgg
     5881 ggaatggtgc gggtggaaag cacgacgata gtgcggtccg caatgccctg ggcctgctcg
     5941 gcggccataa tgatgttttt gttgttgggc agcacaaaca ccgtcttggc gggcacgctc
     6001 tgcacagcgg ccacgatatc ggccgtggag ggattcatcg tttgcccgcc ggacaccacg
     6061 gcgtcagcgc caaggtcggt gaagatggcc ttgaggccct cgcctgcggc gacggccacg
     6121 aagccaaacg cccggtcgtt gtccacggcg gcgtaggtga actcgttgcc cgtatccgcg
     6181 gcctcggcgg cggcctgctt tttcagcccc tcgccctgcg cctggcggga cagaaactgc
     6241 tcgtgcatgt tttcaatttt gaaatttgtc aggtagccgt actgcaacgc atggctgacg
     6301 atcttgcccg ggtccgcagt gtggacgtgg cagttgatga cctcatcgtc ctcgatgacc
     6361 acgacgctgt cgccgttgct ctccaggaag gcgcgcagct tattgctgct ggcaccgtcg
     6421 tttttgttca ccagaaactg ggtgcagtaa ccgtttttga tgtcttcgac cttcatcagg
     6481 tcgtcggtgt aaacgccctt gcctgcgccg ctggtggaga gcttggcgct cttctccggc
     6541 gcttcgtcag cggcgatgat atggttgtct ttaaaaacgg agagcatgcc ctcgaagata
     6601 tacacaaggc cctggccgcc cgcgtccaca acgccggcct ttttcagcac cggcagctgc
     6661 tcgggcgtgt tgtccagcgc gcgctgggcg gcggtgcaca ccagctgcca cagctccacc
     6721 gcgtccgcga tgccgctggc cgcggcttcc tcgctggcca cacgggccac cgtcagcatc
     6781 gtgccctcgg tgggtttcat cacggccttg tatgcaccct caacgccttt ttccagcgcc
     6841 tgcacaaggt cctttgcatc ggctgttttt ttgccctgca gcgccttgga aaagccgcgg
     6901 aacagcaggc tggaaataac gccggagttg ccgcgcgcgc cgcgcagcat ggcgctggcc
     6961 gttacctgtg cggccttttc cacggtgcag tcgtcgggca ggttcagaag ctctgtccgc
     7021 gcggcgccga tggtcatgcc catgttggtg ccggtatcgc cgtccggcac ggggaaaacg
     7081 ttcagctcgt cgaccttggc gcggcggttg gaaaggttgt tcgcgccgga gatgatgctg
     7141 tttttcaaag tcaaaccagt aatcatttcg tttcttcacc tcaaacaatg tttcggccgg
     7201 cggccgggtg cccggacggg cacgcttcag acgatgtcgt ccacgtaaac gtcgatgcgc
     7261 tccaccttca aagtggtggc gtcctccacg gcgtacttca ctttatgagt gatgctgcgc
     7321 acgatggccg caatgttcag cccgtaggtc accttgatat gcagctcgat caccagcttg
     7381 ccgtcctctt ccgtgacgcg tacacccttg tcgggataat tggtgccgag cacgatggtt
     7441 ttgagactgt cggacgtgcc gcccgtggcc atgccgcaca caccgtagca gctctgggcg
     7501 gcggcgccca ccaggcccgc gaaatagtcg ctggtcatgc agaccttgcc aagggggttg
     7561 tagaacttga tcaaaaccga tcactccttt actgcacgcg cggggcctcc gcgcgatgga
     7621 atccattata ttatacaccg caaaggcccg attgtacagg ttttgcacgg gtttatttct
     7681 ttttcgcatg cacgtacgga aatagatgta gtttatttgt gtcgcatatg gtagaataat
     7741 tgaacatacg aaagcagcgg cgggacgggc ccgtgctgcg cgcagccggc ggcggacgcg
     7801 gcgtgcggga aaggaagttt ttatgaagca tgcaaaacgc atcgcggcgc tgtgccttgc
     7861 ggcggcgctg tttttaacag ggtgtgcctc cggtgcgtcc ggctccgctg cgttcagccc
     7921 ggaaaacccc gtcaccgtga cggtctggca ctattacaac ggcccgcagc agcaggcgtt
     7981 tgcggatctt gtcaatgaat tcaacgagac tgtgggccgg gaaaagggca tcgcggtaca
     8041 gcagtccagc caggccaccg taacggatct ggagaccagc gtgctggctg cggccaaccg
     8101 ggaggtgggc gcgccggagg tgcccaatat ttttgccgcc tacgccgaca ctgcctatgc
     8161 ggtggaccgg cttggccttg tggcggacct gcgtccctac ctgacggacg aggagttggc
     8221 tgaatatgtg ccgggctatc tggaggaagg cgcgttttcg ggcgagggaa cgctgaagat
     8281 attccccatc gcaaaatccg tggagctgct gatgctgaat gcgacggatt gggaaaaatt
     8341 cgccgctgcc acgggcgcgg atgacagcgc ctttgccact gtggagggca ttaccgagac
     8401 ggcgcgccaa tattatgagt ggacggacgc tcaaacgccg gatatttcgg acgacggaaa
     8461 ggcgtttttt ggccgcgacg cgtttgccaa ttacatgatc atcggtgcgc gccagctggg
     8521 cgtggagctt ctgcgtgtgg aggacggcgc accggtgctg gatttcgata aagatgtggt
     8581 ccgccgcctg tgggacaatt actatgtgcc gttcatcaac ggctggttcg gcgcgtccgg
     8641 gcgctttcgc agcgacgaca tcaaaatggg caatatcatc gcctgcgtcg gatcctcctc
     8701 gggcgctgcc tatttcccgg atgaatgcat cctcagcgat accgagagtt atcccatcga
     8761 gatgcgcgcc tacccatgcc ctaaattcga gggcggggaa gattttgccg tgcaacaggg
     8821 cgcgggcctt gttgtgacga atgccgctcc gcaggaagtt gaggcttcgg tggaatttct
     8881 caagtggttc acagaagccg ggcgaaatct ggaattttcg ctgggttccg gctatgtgcc
     8941 ggtgaaaaaa gaagccaatg aaatgacgtt tgtggaacag gcgatggacg cggcaggcgc
     9001 cagttccaaa atgcgcgcca tcatcacgaa cgctatcggc atgacgggcg agaaccagat
     9061 gtacactaca aaagcattca ggaacggcac tgcggcacgt gccatcctca acgattccat
     9121 ggtggaaaag gcacgggcgg accgcgaggc gttccttgcg ctgacggcgg agggcatgcc
     9181 gcacgaagag gcggccgcgc agtttgtaac ggatgaaaat ttcgaggcgt ggtatgagga
     9241 gacgcgcacg gcgctggaga tgtgcatcgc gggctgacag acagataatg gctggaaagg
     9301 cgggacagcg gatgaatggg gaaaaaaggc gcaggcatgg gcggtcgatc cgccgccgcc
     9361 tgctcgtgcc catatgcttg ctgctgacgg tccaggtggc cgtgctcatc ggtggagttt
     9421 atttttcagg cctgctggaa cgcatggacg aaaatgcata cgcggtgctg gaccagcgcg
     9481 tacagagccg ggcgggctat ttggaagacg atatggtgcg ccgctggtcc aacctcagcc
     9541 tgacgcagga ggtattgggc gaactgtacg agggccttgt gcaggacggc tccgtcacgc
     9601 ctggagagct ggcgtcggac cccgcccagt acaacgcgtt tctggaacag gcagcgccgg
     9661 aactcgtctc tctgatgcgt acaaacagcg tgacgggggc ttttgtcgta ctgaacacgc
     9721 agacgttccc gcaggaactg agcgagagcc tgcaattgcc cgggctttac ctgcgcgaca
     9781 gagacccctc cgcccacacc agcgccggca acggcgatct gctgctggag cgcgcgccgc
     9841 tggaactggt gcgcggcctc ggcatcgcca tggacagcaa ctggaagccg atgttcgtgc
     9901 tggaggggga caccgcgcgg gccgatgcct gcctgataag accctacaac gcggcggtgg
     9961 aggacccgtc ccttggctgg gaaaatgcgg gctattgggc tgcgccgcac acgctgagcg
    10021 gcgataacat ccgtctgatc tcttattcgg ttccgctcat cgcgcccgac ggaacggtgt
    10081 acggtgtttt gggtgtggat atcctcgagg actacctgct gcggttgctg ccttacgacg
    10141 agctttcaga ggatcacggc agcgcttaca tcttgggtgt gcgcggcgcg gccgacccgg
    10201 aagcatccgt ccggccgcag gtggtgagcg gtcctgtcta tctggcggct tccgggttca
    10261 gcaccgtata tctggaaaag gagagcgcag gcctttacac gctggagcgg ggcggcggcg
    10321 aggagatgta cgtctgctcc gccgtgctga acctgtacaa tacgaacacg ccttttgcag
    10381 acgaccagtg ggttctgctg ggggcggtgc cgggccgcac gctgttcgca ctcaagcacg
    10441 atacgctgct gattatgctg gcgctgctgg catccatcgt tctcaccagt ctggtgacga
    10501 tcctgatcac cgtacgttcg gtttcgcagc ccatcgtcaa gctggcggcc gaagtgcgcg
    10561 cgacggaccc ggcggctccc gcccgcctgc aaaaaacgga tatcacagag ctggacgcac
    10621 tggcgggctc catcgagacg ctcagcgacg aagtgctgcg cgcgggcggc aagttcaccc
    10681 agatcctgga gatggccagc gtgcgcatgg gcggttttga ggtggacggc cgggacggca
    10741 cgctgttcct caccgaccag tttttcgacg tattcggccg tccggagctg gcgagcaccg
    10801 tgcgcggccc acagtccttc gagagagcga tggatgccct gcacgtctat gtgacggaac
    10861 gggaaaaaac cgacgacgag catacggaat acattttccg catgccggag ccggacggcg
    10921 tgcgctgggt gcgtatgcgc ctgctgcgca gcagttcccg ctggacgggc ctggccgagg
    10981 acatcaccag tgacaggatg tcgttggaac gcatggaata cgagcgtgac cacgacgtgc
    11041 tcaccgagct ttataaccgc cgcgcattca aacgtatggc gctggcgttg ctggcccgag
    11101 ggccgcaggc ggcgtgcgtg ggtgcgctgg tgatgctgga tctggacaac ctgaagtata
    11161 tcaacgatac ctacggccac gatatgggcg acgcctatat ccgctgtatg gccgcgagcc
    11221 ttcccagccc cgacggggaa cgctgccttg tggcacgcat gtccggcgat gagttcttcg
    11281 tgctgttcca cggttatgac acacaggaag aagtgcgcgg gcagatcgcc ctgctgcgcc
    11341 agcgtatgac gcgggcttcg cttacgctgc ccgacggcgt gcgccagccg gtgcgggctt
    11401 ccggcggcgt ggcttggtat ccggcggaca gcgaccggtt cgacgagctg gtgcgctatg
    11461 cggatttcgc catgtaccag gtgaagaaca gcggcaaggg cgaatttgcg gaattcgacg
    11521 gcgaggcata ccggcgtgac gcctatctgg tgcagagccg cggcgtgctg caccgtttgc
    11581 tggagcagcg gctggtggat taccacttcc agcccatcgt agaggtgagc acgggccgca
    11641 tttttgctta cgaagcgctg atgcgccccc gcacgccgga gctgtcctcg ccgctggccg
    11701 tgctggaact ggcgcggcag gagggcaagc tgcgcgcggt ggagactctt acctggcata
    11761 cggccatggg cgcattcgcg gcgcactgcc gtgcagggca cgtcccggag ggctgccgcg
    11821 tattcatcaa ctccctttcc aaccaggcca tgctgcccga ggatacggcg cggtttcatc
    11881 gcgaataccg ggactacctg tcccgcgtcg tgctggaact gacggaggtc acgcgcatag
    11941 atgccgatct tcaggcggta aaaatgcagg cattgatgcg cagcggcgga aagctggcgc
    12001 tggacgatta cggcagcggc tataacggcg aggccatgct gctggctgtg cagccggatt
    12061 atgtgaaaat cgatgttgcc atcgtgcgcg gcgtggacca agacgacgac aagcgccagc
    12121 tggtagaaaa tctggtgtcc tacgcccatg tgcgaggcat agcgctggta ggagagggtg
    12181 tggaaacgcg tgccgagctg gaggctctgg ctgcgttggg cgtggattat gtgcagggct
    12241 attttctggc ccgcccggct ctgctgccgc ctcccgtcac gccggagggg gctgaatgtc
    12301 tgcgtgcgct gcgcggggaa atggattgac cgccgccggg cagaaaagtg gaaaacagaa
    12361 cagagcgccc cggcgcggcc tgaaaaggcc gccggggcgc tctgctttcg ttcgggtcgc
    12421 gccgctcaga agttggcagt gaaaaattcc ctcagctcct gcagggtggc ttcttcgtct
    12481 tcaccgctta cttctatggt cacggtctcg ccgcatttgg ccgccagagc catcacgttc
    12541 aggatgctct tcgcgtccgc actgcgtgtg ccgcatcgga tgacgacgga acagggcgtt
    12601 tcggccagcc gctttgccat cacaccggcg ggacgcatgt gcaggcccat cgggtcggtc
    12661 agggtatatg caaaactcac caatgcaaac cgctcctttc cagccgcgca gaactgcgcg
    12721 gagcccaggc aaacgtcttg attactcttc tactatacaa taaaaggaag cgtatgacaa
    12781 aggggttttt gtgttttttt gcggtggcgc gtcgttttta tcccgccgcg cggggcggtt
    12841 cctgcgtgtc gcccgtatcc ggcatgctcg gcatccgccc atgcccgcgg gtgggctgcc
    12901 gggttgcagg gcgggggatg gcgccgctgc gcagcagcgc aagctttgcg caggccgggc
    12961 cggtcagctc atacagtaca gcggaggaaa ggatgatggt ggccagcatc gtgccttcgg
    13021 ccgcaggcag catgcgttgg cccagcacgg caagaccgat ggaaacgccg gcctgaggcg
    13081 tgagagcgag gcccagatat ttgatgatgg acgggtcggc gcggcacagc gccgcaccgc
    13141 tgctggcccc cgcgtatttg cctgcgatgc gtacgaagaa ataggccacg ccgataacgc
    13201 ccgccgttgc gagggacggg atgttgagac gcaggccgga aagcgcgaag aacagcagca
    13261 ggaacggcgg agagaatttg tccatccgct tgaacagacg cttttcgccg cccaggttca
    13321 cataacccgc gcccagtgcc atacaggcca gcaacggtga aatgcccagt gtcgagcaca
    13381 gcccgctcaa aaacaaaagc accgcgttga cgagtaccag tgtgtgtgcc tgggaatgcc
    13441 cctgcccggc aagccaacgc agcagcacgg ccagcgccgg ccccagcgcc accgccccca
    13501 gattccacag caggggcagc gccacgtctg caaactgtat atggcctgtc tgcagcgcgt
    13561 tcaccacggc ggtacaaacg ctgaaggcgg tcagggccac cgcgtcgtcc aaagcggtca
    13621 cctgcaaaag caggttgacg aaaggtcctt tggcgcggta ctgccggatc gtcatgatgg
    13681 tggaggcagg ggccgtggcg cagccgatgg cgcccagcag cagtgcaaac ggcacggaca
    13741 ggcggaatac gaagatcatc gtcagcgtga cggccacggc ggcgcacagc gcctcggcca
    13801 gtgtgacagc cagcaccttt ccgccggaac gctttagatc cgcgatacgg aaataccgtc
    13861 cggcggaaaa cgcgatatag gagagcgcga gatcggtgac gaattccatg ccgccggaca
    13921 gccatgcggg gatcaggttc agtgcatacg gtcccaccag gatacccgcc agaatatagc
    13981 ccgtcacgtt gggtaaatgc aggcgccgcg tcaggcgcgt catcaaaaaa ccggctgtca
    14041 gcagaatggc gacgcccagt aatacgccgc cagcgctctg cggcaaggta aaaatgttca
    14101 tcaagcacac ctctttctgt tgtgtgcttg attatatgcg cacactatga taaaatcaaa
    14161 ttatgacttt tcatgtacat ataaattttt ttaatgaatt ggggagcaaa atgtttgatg
    14221 cgaagataga gaccctcctg gaggtcatca gtgcgggcag ttacacacgg gcggcacagc
    14281 ggctttcgct cacacagccc gctgtcagcc atcatatgcg ccagctggag caggagttcg
    14341 gcattcatat attttataag gataaaaaag agctgcgcct gacgccggaa ggcgagattc
    14401 tggtaaaata cgcccgccgg gcgctggctg tataccgcaa cgcccgccag gccatcgagg
    14461 acagccgcat ctgcatgtcg cacatcgggg tgggcatcac ccacaccgtg ggcgaaagct
    14521 gtgtgccgca ggtgctggcc gaatactgca accagacgcc cggcgtacac atcaatatcg
    14581 ttacggacac cataaaaaat ctttatgata tgatgcgctc ctacgagctg gacgtagcca
    14641 tcgtggacgg gcagttcccg gcatccggct acaccgaagt gctgctggat accgattacc
    14701 tgtgtcttgt ggtgtcgccc atccacccgc tggcacggcg tgccagcgtc gccatcggcg
    14761 cactgcaggg cgagcggttc atcatgcgtc ccaagggagc gggcacgcgc tcgatgtttg
    14821 aaagttatct gtccgaatgc ggggcggata tccgggactt caatatcatc atcgagatgg
    14881 acagtgtcgc caccatcaag gagcttgtcg cgcaggatct gggcatttcc gtcatcgccc
    14941 acagcgcctg ccgcgaggac gagcaggcgg gccgcctggc agtcgtcccg ctggagaagc
    15001 cgggcatggt gcggcgcatc aatatggtgc atcatcagga tttttcccat ccggagatcc
    15061 tggaggagct gcgccgtcta tatatcggcc ggcgctgagc cgggggaggc acggaaaaag
    15121 cttgtaaaag gggtggagaa cccctttttc atttaaaata accccgcgac gccccgcaga
    15181 gccagtgccg aggcgcgtaa aagcgaagct ttttcgacaa gctgaggcag ggctttacga
    15241 ccctgccttt tgatgtttta cactgcaaaa cgcgaggctg gctcacaggt ccagcgcggc
    15301 aaagctgtgc atggcgcgcc gcaatgccag cgccgacagc agcacgtaca gtgtggcgcc
    15361 agcggccaat aaggtgagct tttcgcccag gaacgccggg tccggcgtgt ccaggcggtc
    15421 ccgcacaaag gggacggtgt gggcgcaggc ctcgaatacc accatgcaaa gagtaagggc
    15481 gccgcagccc caaccgaagg ccttgccgac cttgcccggc gcgcggtagt agtttcggaa
    15541 aaagacaaga ttgaacacgc cgtacagcac cagcgcgaat ccgaaaaacg cggtgttcgc
    15601 gtccatgccc acaaggtttt ccgcggagac acggctgctg aacacggcga agggaacggc
    15661 gatcacgagc tgtgccagct gcatcagcat caccgtaaaa acgcgcgccc gcacaagatc
    15721 ggtcttgcgc acgggcagca gcgccgtgaa ctcgatgtcc cggttctccc gccccgtcag
    15781 gcagatgaaa aaggtgccca ggcaggtgta aaagcaaatg acataataag gatagttggg
    15841 gatgagcagc attgcggaaa gcagcaggaa cagcattgaa gccggatgca tggccagcac
    15901 aagctccttg cgcatcaggt ttttcatagg ccgccgtcct cctccttttc cagatgtacc
    15961 ataatatcct ccagcgatgc gggcgcggta tcggcgccaa ggcggggcgc gtcctcggcc
    16021 cgcagcagag cgctgaagcc ttcccgctcc cgtttgcatc ccagcagcag gccctcgggc
    16081 gcagcgtccc gctgcgcggc tgtcagtgcg gctgtgcggt accgggcgcg cagtgcgtcc
    16141 gcgtctgcgt ctgccgccac ctcgccctgc cgcaggtata cgatgtggtc ggcgcatttt
    16201 tccaggtcgg aggtgatatg cgtggagaac aggatgctga tgccttcgtc ctgggcaagc
    16261 tggagaaaga tctccaacag ttcgtcccgg gaaacgggat ccagcccgct ggtgggttcg
    16321 tccagcacca aaagccgcgc ccggtgagag agtgccagag ccagggcgta ctttaccttc
    16381 atgccctcgg aaagctcttt cactcgtttg ttttcatcca gcgcgaacag cgcgagatac
    16441 cggcggtaag ccgtctcgtc ccagctcgga taaaagcggc gcgttacctc cgttacagtg
    16501 cgcaggcgct taaggggata aaacccgaag cctccggaca caaaccccac cgcctgcttg
    16561 acgccgaatt catccgcgga cagcggcctg ccgaaaaaca gcgcctcgcc ggcgtcgggg
    16621 tgtacaaaat ccaacagcga tttcagcgtt gttgtttttc cagcgccgtt gcggccgatg
    16681 aagcccgtga tggaac
//
LOCUS       DAWBSB010000021        15446 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_973_length_15446_cov_4.180820, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000021 DAWBSB010000000
VERSION     DAWBSB010000021.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 15446)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 15446)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15446
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_973_length_15446_cov_4.180820"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    14159..14168
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    14882..14891
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2919 a         4849 c         4695 g         2963 t
ORIGIN      
        1 gtactggatg tggcgctacg gtatggctat gagtctccga cggcgtttaa ccgcgcgttt
       61 caaagcgtgc acggcgtttc gccgtctgca gcgcagaggg acggcgcacc cctgaaagcg
      121 tatccccgta tcagcttcaa gatcaccgtc aagggggaag ctgaaatgga ttaccgtatc
      181 atcaaacaag aagccttccg tatcgtgggc gtgcgcgaac cgcttttgcc ggatttcgag
      241 gattcgttcc gtcgggtgcc ggaattttgg ggcgaagcgg cggcgtccgg caccataccg
      301 cgcctatgcc tgctgatgga cgccgcaccg aaagggattc tgggcgtcag cacctgtatg
      361 ccggacgcgg aaaattatta ttacatcgct gtggccagca cacgtcctgc gcccgaggga
      421 atgcacgaat atgtggtgcc cgcctgtacc tgggccgtat ttccaggccg gggttccatg
      481 cccgccgcca tgcaggagct gcaaaagcgc gtggtttcgg aatggctgcc cggctcgggc
      541 tacgagtggg gccaggcgcc ggatctcgaa gtgtacctgg acgacggcct gtccggagaa
      601 agcagctttg aggtttggct gcccgtcgca aagcggtaag agccgtgtgc gcagcaggga
      661 aacggcgcag ctgaggatag attccggccg ggcaggaaac gcccggccgt tttttgtccg
      721 cgtgccttga ttttccatgg actcccgctt gacggcgggc ccggcggcgt gtacaatgaa
      781 aaattaggat agtatgccga aatgcgcggc ggcgcggcgc tccacggcag aaaagggggc
      841 ggtgcgatgc cgcgcggaaa acgtgcgtac cgtgtgctgc tctgggccgt tttggcggcg
      901 ctgctggccc tggcggcttt tgccgggtgg cggctgtggc gcgtgtacgg cgacgcggcc
      961 cgtgcggcgc agctggcgcg ggaaatgcag cagacggccg tgcctgctgc cggtgagagc
     1021 gcggcgggca cagacggggc ggaaatgccc gcccatgctt gccctgtgga ctttgagacg
     1081 ctgcgccggg attatccggc cgtgacggcc tggctgacag gctgcggagg ggacatcgat
     1141 acgcctgtgg cccaaggggc ggacaacatt tattatctgg accatctgcc cgacgggacg
     1201 cggaacatgc tgggcacggc gtttctggac agcgccaaca gcgcgtcctt ttccgacgac
     1261 cagtctttta ttttcggcca tcatatggac acgggcggcg gtgtgttcgc accgctgctg
     1321 cggtatcaga agcaggaata ctttgaaaaa aatcccacgt tcacgctgca tacgcccggc
     1381 ggctgctaca cggcctgggt attcggcgcg ttcgtggtac aggagcgggc gtacccatac
     1441 gtgcatgagt tcacctctgc ggacgggatg gcggcatttg tggagcgggt gcgcgcgcag
     1501 tcggatatca tctcgccggt ggaacccgcg ccgggagaca gggtgctcac attgtgcacc
     1561 tgtacgtcga aaacaaacga cctgcgctat gtggtgtgcg cgaagctcat cccgctggcg
     1621 taaggaaaag cagaaaagcc ccgttccggg ggcgtgaaag gagggctgcg gcatgcgcag
     1681 gaaaacagaa cggcagggca ccgaggccga gcgccagctt gcatggagca gggaatacct
     1741 gcggcgggaa aagcgccgcc gcgtgctgcg ccgcacactg ctgatgctgg tgctggcgct
     1801 tgcctgcgcc gtgacggtat ttgcgctgaa cggcggcatt cccgcactgc gggtgggtgg
     1861 cgcgcctgcc tccggttcgt ctgcggcgca gtcctcgtcc gcgccggccg tacaggcgca
     1921 gcccccgtcg tctcaggcgc agccggagcc ggcggcatat accgtcgcct tcgacagccg
     1981 cggcggaagc gccgtggagg cgctgcaaaa tgtgacgcag ggcgcgcgca tcgccgagcc
     2041 gtcgtctccg gtgcgggagg gctatacgtt cgagggctgg tacagggacg ctgcgcggac
     2101 ggacgcttgg gactttgccg cggacacggt ggagcaggat accattttgt atgcaaaatg
     2161 ggaggccgtg cccgcgcagg acacaacttc cggcgcggct ctgccccaga cgggcgtggc
     2221 cacgggcgca ttgctgtggg cgtgcgtgct ggctgcggcg cttgcgctgg ccgcggcggc
     2281 agccttgctg ctgcgcaggg cttaccgggg ctgaatcgca gaagacaggc caaaaagccg
     2341 ctgcaaacgc agcggctttt gttgaaagaa aaacggaaag gcggagaaag gccatggacg
     2401 ccggatttat aaacctgacg ccggaaaatt tggacggcga acatctgtgc tgcatcatac
     2461 gcagcaaaaa agcgcatccg ggcgtggaag cgaaacggca gtggctggcg gaccgcttga
     2521 aggagggcca cattttccgc aagctgaacg caaaggctac ggtattcatc gaatacgccc
     2581 ctcttgaaac cgcatggacg ccggtgctgg gagacagcta ttactatatc tactgtctgt
     2641 gggtctgcgg ggaaagcaag ggcaaaggct acggacgggc attgatggaa tactgtctgg
     2701 ccgacgcccg ggcaaaagga aagtccggcg tgtgcatgct gggcgcggaa aagcaaaagg
     2761 catggctttc agaccaggcc tttgcaaaaa aattcggttt tcagacagtg gataccgcgc
     2821 caaacggcta tgagctgctg gcgctttcct tcgacgggac aaagccccgc tttgcgcccc
     2881 atgcgaaaaa gcaacagatc gaaaacaagg agctgacggt cttctacgat atgcagtgcc
     2941 cgtatatcct tcagcgcgtg gagcgggtgg aacggtactg cggggcgaac ggcgtgccgc
     3001 tgtccctcgt ccgggtggat acgctgcaaa aggcgaagga gctgccttgt gttttcaaca
     3061 actgggccgt attttacaaa ggaaagtttg aaaccgtgaa cctgctggac gacgccgcgc
     3121 tgcaaaggct tttgaaaaaa tgagaccggg tatccgaaag gggtgcccgg ccttttgtac
     3181 gcaagaagtt ggagcaggcg ctccgcagca aagacagagc cggcgttatg gcggaagcct
     3241 gcttcaaggc ggcgggctgg tgctgaaagt cctgtttgcg caaggggagc tttggcgtgc
     3301 ttttttcagg tgcatcccga taaatcggga tttggtcagt ggatcacttt ataacgcaat
     3361 cgtaaatacg actttttcaa aaccatgcat tcttgcagct tcagcacgcc aatttttgat
     3421 aattcacttt cggacaacag ggattttccg tcaatgagtg tgggcgtatc tttgccgccg
     3481 atcaaaacag gggcgatgac gatatcaata taatcgaata acttttcatg aagaaataaa
     3541 ctgtttaacg tacctccgct ttgtatcgtt attctttcac aaccgtattc tgatttgagt
     3601 tcagcaagtg cctctttcag tgagggacca ttttggcgta tgatatgcag attgctttca
     3661 tctacatcaa aggccggatg gtttgcatta gacgtgatta atacaaattc ttttgctaag
     3721 gaacaaaaat agagtacgcc atttttgttc aaatgatcat tatcaattat tacaaaggaa
     3781 acgggtgttt tgtcgggcat ttcttttgtg ttgacaccca gtttagcttg gacgcggcca
     3841 gaattgagcg accacagatc tgtcgtctgc tctatttcat aatattgatg caacccttca
     3901 cgaacccccg caattttagg aaagtctttg tctatgtcca aatcgtctgt cgcaccagtg
     3961 cttattttgc catcaacgga cattaacata aacaatgttg taattggtct gtccatgcta
     4021 tccctccaaa atgtcgtttg ccgtcctgtt ttattatatg ggagaatagg gcgtccctca
     4081 agaaaaacct ttataaagga tacagaaaaa cgggtgcaac cggccggttg cacccgtaaa
     4141 tcgttttacg gcgctgcatc ttttttgggg ggggattcat tcttctgtca ggtaaggcag
     4201 cagcacgggg agaagaccgc gggccatgaa ataaaagccc aggtccgtgg gatgcacgcc
     4261 gtccacagta tattcttcac cgccgtccgg ccagagcgtg cggccgtcta ggaaagcgat
     4321 atttgcatcg ccgcccgcgc gtgcctgctc cacagcgtca cgctgcgccg cccgccaggc
     4381 atccttttgc gccacgcggt ccggctgttg ggcctccaga ggaccgagcg gcgcgctgac
     4441 caccagaatg ggcagccgcg ggcatgccgc gcgcagtcgc gccaggaaag cgggcaggcg
     4501 gcccagacag ccaagcggcc cggcattggc ttcagcgtcc accaccagca ggcgcaggcc
     4561 cggcacgcgc gccagtacgt ccgccatctc cggctccagg catgcgttgc ccgaaaagcc
     4621 gaagttatag atctgtacgt ccagcgcacg ggcaaggatg gcggggaaag ccatacccgg
     4681 atgggaagcg cagccgccct ggtcgatgga cgtgccgtag acggcggccg cgccgcttac
     4741 ggcacggggc gcgggcgcgg ccacagaggc gtccgcgtca acgcccaccc atacctcttt
     4801 cacgcccatg tacagcggaa aatgaattag aaattccttt tcctcccggt cccctccgct
     4861 ccacaatgcg gcggtatatg cggtttctcc gtggccgaac acactgggcc cggcgtaacg
     4921 ccatgcgccg cccggcctgc gcagatagca gtcaaaaccg ccctgtccgg tggagggcat
     4981 gtggtgcatg gcgcaagcag ccgcaagacg tgctcggaca agcacgcgtc ccgtactgct
     5041 gcgaaaacgg atgcagccgc ccgctgtatt tgtgcccagc tcggtgaggg cggctcgccc
     5101 ggcgcgggaa aataccgcgt cggaatccag cggcaggcgg cggtatgcgc cgttctgctc
     5161 gaaaaaggca aatccctcca gcacgagggg cggttcaaat ggagcaagcc accgcacgcc
     5221 ctcctcctgt gcggggcgcg cctgcataaa acggtccagt tttgcaatat ccatccgtcg
     5281 gcctccgtta ctttttcttc cggttcagct tcagacatat gccgtttgcc gccgcggcct
     5341 cctgcagcag ccgcgcggcc agcgcgccct tttcccggag ggcggcgtcc gggggcgtct
     5401 gccgccgcag cagggacacg gtttcccgta cgtccgcaag ctccagcagg ttgacggccg
     5461 tgcagaacag gcttttccgc cgtccgtcgt tgtattcccg tagcaaaaag cgcaagagcg
     5521 cctccttttc ctccagcatg gcccgatatg cgtccagtcc gtcccgggcc gcccgggcaa
     5581 aatctctttg cacattgcgg tggggcacga aggagtcata aggctcggcc ctggtgtaac
     5641 gggcgcaggg atattccgtg cactggaagc agtattccac gccgccgtgc tcccgcgcgc
     5701 accgctgcac cgcgcaggag ggacggcccg cgccgccgca gcccgtgcag tgctccgtct
     5761 cgctgatgtg gtacatcggg cacagcccgc agttcaggcc gcacagggaa aagcggggct
     5821 cggcccgcgt ctgttccttc atacaaaacc tccctcaatc gttgaagcgc gccgcgcccg
     5881 gattacggcg tgccggccgg ttccggcggg cagggccgcc cgttcagaga tgccgatacc
     5941 agcgtgctgc cgcggtatac cagcttaaga gagaaaaacg gcgcttcctg cgccagcagg
     6001 cgcaggaaaa tgcggtcgcc ctcccaaagc ggcaggcccg gcaccgcgct cttttccacc
     6061 catgccagct cgccttcgac acacggcccg gccaacgtac cctcgaacgc gtccgaggtg
     6121 aaaagatgca tatactccgt gggccacacg tccgacacga acgtcacaag cccccggtac
     6181 cgccagtccg tcagcatcag gcccgtttcc tcccgcacct cgcgcagcag gcagtcttcg
     6241 gggctttcgc cctcctcgca cttgccgcct acaccgatcc acttgccctc gttttcgtcg
     6301 ttggcctttt tggtgcggtg cagcatcagg cagcggcttc cctgttcgat gtagcacagc
     6361 gtcgtattct tcattgccgg ccccgcgctc agaagtttcc gccgttccag ccccagttgg
     6421 gcgtttcggc gtatttgacg tatacccgcg cggggtcgat gtccagcacg gcgtccagca
     6481 gttcgcagac gcgggccgtc agtgcatcgt aggcgtccgg ctccgcgccg ccgtaaacgg
     6541 atacctccac cagcgcggcg ggggcgtcgg tgccctggaa ccacagcgcg cactcgggct
     6601 ccacgcccac catcagccag gcctcgcttt tgccgggaat ggcctcgatg gcctgcccca
     6661 gccgtgtctt aagcgcctcc tgtgcgtttt cggatacggg gatattggtt tttacattga
     6721 tgaacggcat aaatatttcc tccctgacgc gccggtcccg gcgcgctttt tgatgcagcg
     6781 cgcggcgctg tacgtcagcg gcgtagccag ccggcggcca gatccaccca gcccgtgcac
     6841 tccgcctgca cgcccgcgcc ggcggtgttt tccgtcagct cgttggcaag ggacaacccg
     6901 tgcccgccca cggagtatat gtgggactca aacggtacgc ccgcacggcg cagggccatg
     6961 gcgaacagca tcgtgttttc cacgggtacg ccgggatcgg tataggtatg ccacaggaac
     7021 gcgggcggcg tgtcctgcgt cacatgctgt tccagcgata cctctgcgcg gcgggcggcg
     7081 tccgcgtcgg cgcccagcag attgtcgaaa gaccccttgt gggcaaattc tccgccggtg
     7141 ataacggggt agcaaagcac cattttgtcc gggcgcatgt cctggggggc aaggccggtt
     7201 tcggcagcga ggaacggtgc gttccaaaac acaccaaggc tgcccgccag atgcccgccc
     7261 gcagaaaaac cgcatacgga gatttgccgc gggtccacat ggttctccgc ggcgcccgcg
     7321 cgcacccagg ccacggcttc ggccagcgcg cgcagcgcgg cggggaaacg ggcgggcgcg
     7381 cagggatagc gcagcacaca ggcgtggaag ccgcgggcgc acagggccag cgcgatgggt
     7441 tcggcctcgc ggtcgctggt ataggcgtag ccgccgcccg gacatacgac aacggccggg
     7501 cggcacgcgc ccgccttcag ctcgggagaa tccgccagaa aataggtgtc caggtacgcg
     7561 ccctgagcgc cgagagggat gcgttcacat ttcataacaa ttgctccttt gtcaatggga
     7621 tgcgccggcc cgggccggag acagacggaa aagaaaaggc gcccgcagcg ctccggcacg
     7681 gctgtgcgcg gggcgaaggc gggcgcggga acgcggcagg gcggcgctca ttccagcggc
     7741 ggcgcaacaa acgcctcgcg gccggtttga gcggcttcga caagggcgtt taaaatctgc
     7801 accgggcgca gcagcgtgcc gtagggctcg ggcggggtgc gcgtcagcac gccctgcacg
     7861 aattttgcga acccctgccg gtagatggtg gaaatgtcga tggagtgcac tgccacttcc
     7921 tttgcgccgt acagcgcagc gtgaaactcg ctcacctcgg cgaaattcac gcacacgccc
     7981 agcgacgcgt acttgaacac ggcgatgaca ttgcccgccg taacgtcggc cttcacactg
     8041 atgatatccg gcccgaaggc ggttgtcaat atctcagcgg tatggccgcc gtaaaaatac
     8101 agcccgccgt aagggctgtc caactctccg gggaaattca gcccggccga gatcacgccg
     8161 ccgtccgcgc acatggcgcg gtatttgtcc cgcagccaaa gcacatccgg gcagtatttg
     8221 cacgtggagc cgcccgccag cagcgcgccg ctctcccgcg cggcggccac cagagcggcg
     8281 gcctggccga tgtcggtggt gaaaggcttg tccacccagg tggagatgcc cgcgcgcagg
     8341 aagggcagcg ccgcgtcatg gtgctgcgcg ccgtggcgca gcaccaccat cacggcgtcc
     8401 acctggccca gcatacgggc cgggtcgtcc acaacggtgt ggatatgcgc ttcgtttgcc
     8461 acgaaggcgg cgcgctgggc gctctcgccc cacagcatgg tgacgcgcgc gggcaagccg
     8521 ctgccgccct cctcgggcgg aagattggcg agcctggaaa aagcgagggc gtggaagttg
     8581 tccgcgccga tcatgccaat gcgcagcata cggaatccct ccttacagat tgatcacggc
     8641 aatggcggcc acgcccagca aaataccgag cctgccgcgc gccgaaagtt tttcccggaa
     8701 cagcagcacg gagcacagcg tcacgcccac aatgatgctg ccctgtacaa gcgggaacag
     8761 gtagctggca tccacgcgcc cggccagcca ggtgagcagc agattgccgc ccacagagcc
     8821 gcccgccaga agcacggcgg caagagcgtt ttggcgcagc agcgcggtgc cgtccgccgc
     8881 gcccgcgggc agcctgcccc gcagcgccag atacgccagc gcgtagcagg ccgcggctgt
     8941 gcagaagccc accagcatca ggcccgcggc ctcggcgccg cccgtggcgt tttgctggga
     9001 cttctgtacc accgcgcaca gtccgttgca cagaaaggtc agcgcacaca gcgccagcca
     9061 gcggggctcg ggcttctgtg cgccgcccgg cgcgacgttc aggcaccaga acgcggctag
     9121 gaacagcgcc acggctgcgg caagcgtgaa agtgagcgct tcgccgaaca gcagcacgcc
     9181 cgcaaacgcg ggcatcagca tgctggcgga aaaataaaac gttgtgtagg agagcggccc
     9241 gctggcgatg gccaggttgt aaaacaagat cgtcagtgcg aacagcacgc cgaaggcaat
     9301 gccgtgccgc agcgtcgcgg ggcttacggt gaacatgtcc ggcacagcca gacggccggc
     9361 gcccatggcc gcggccgcca gcagcgaaaa cgcgcagttc accagaagct tctggggcag
     9421 atgttcggcg tgctgcagct tcatggaaag cgtttgcagc acgaacagga tgatcgtgca
     9481 gagaaggaat acagctgtca tgcagcggcc tccagtcgga tgatttttcc ggcgcttatg
     9541 ctgcgccgcc gtcagccctc gccgggctgc gccagaatgt cccgccacag cgcggaatat
     9601 tcgtcccgcg tgcggcgcgg tgcgtccgcc agtacgctgc ggccctttgc cgcgccgccg
     9661 gccagcaggt cgatgaccgt cgcggccatc agcttggcgg gcgccagata ggcgagcgct
     9721 tcatcggcga cggcaaagtc cctgctgtgc gccgcgccgg aaaagccgct caccgtcggc
     9781 tggataacgg gcagcaggta ggacacgtcg cccatatccg tgctgccgca gaagggcaga
     9841 ccgcgttcca cggcaagccc gggcgcgcaa accgggacgt tggccgcgaa cagttcggaa
     9901 agcgccgcgt tctgcgccag cggcagataa cccggcatat cgtcgatctc cacccgcgcg
     9961 cccacggcgt aagcggcgcc ccgggccgca cggttcactt tggcgttggc ggtgcgcatg
    10021 gcctgcacgc tggcggcacg cacgtagctc tccatgcgca catctgcggg cacggtgttc
    10081 accaggtccc cgccctttgt aatgatagga tgcacacgca catggtcctc gtcgcggaag
    10141 gtctcacggt tggcatggat ggccgcgatg gcaagggatg ccgcgttcag cgcgttgacg
    10201 ccttcccagg gcgcgccgcc cgcgtgtgcc tctttgccga gaaaccgcag ctccttgccg
    10261 ataaagccgc ccgccgcgcc cgcaaccacc gcgcgaggct gcggggcgtc tgtttcgctg
    10321 tgcaccatca tggccatgtc gatatcgtca aaagcgcctt ccgcgatgag ctgctgctta
    10381 ccgccgagat actgtacttc gcccgcgcgc ctcagcccgg cccgccagcc gatctcaacg
    10441 tattcctccg cgggcgccgc cagcaggcat acgctgcccg caaggctgtc cgccacggcc
    10501 cgcaggccca cagcgcagcc cagcatggag gcaagctggg cgttgtgccc gcaggcgtgg
    10561 gccgcgcctg tggctttgtc cgcaaacgga tggccggggc tgagcaccgc gtccagctcc
    10621 ccgataacgg ccacccgcgg ccccgcgccg cgccaaagcc agcctttcac gcctgtgcgc
    10681 gcgggccgcg taacgtccgt cagacccagc gaggcaaagg cctccagcac ctttccggcc
    10741 gtgcgcgttt ccttatagcc cagctccgga tgggcaagga tatctcgccc gaaagcggtg
    10801 aggcgctgtg cctgtgcgtc gatggcggcg cacgccgcgc gcttgagcgc gtctgtcatt
    10861 gtgttcatag tccgctcacg ctttccacgt ttcaagattc tgcgcaacga atgcatcgtc
    10921 gttgagatga gggtacatct catacacgcg gtcgatctcc cgggcctgcc cctcgctgag
    10981 ggtctcgtcc gggtccaggc accacgtgcc cgccatcagg ccctgacgcc gcagcacctc
    11041 atggaccccc gggatgcatc cggcaaagtt gtgcgccacg tcgaagaaca cgccgttgca
    11101 gtctgtgacc gccgcgcccc gcgccagaag ctccgccggg atgtcgcggc cgtcccggta
    11161 ttcccgcagc tcttcgtaca gcttcacaac gctgtgcgtc cacaccgacc agtgccccag
    11221 caggcccccc acgaaccgca gctccacctc tttgccgtcc accgggaacc ggtacggcgt
    11281 caaaaggtcc gccacgatgt tgtcgtcgtt gcccgtgtac agcgcaattt cctcccgccg
    11341 cgcgctgtac gccacgcccc gcaccaggtc gatgctctgg taccggttga acgaagcgca
    11401 tttgatggcc accacgcccg gcgtgtccgc cacggccttc cagtattcat acgacagccg
    11461 ccgcccgccg caggccgtct gcaagtaaaa cccgatcacg ggcatctctg ccgccaccgc
    11521 atgggtgcgt tccaccagct cctcctccgt ccggtgcgcc agcccgcccg ggctcagcaa
    11581 taccgcgtcg aaccccagcg cctttgccgt gcgcgcctcc gcgcacgcct gctccgcttc
    11641 accgcacgcg cccgccacgc gcaccagcgt tttccccgtg cgcgcctcgt atgcgtccat
    11701 ctcctctttc gtcacacgca ggaccggctc gaacaggccc acctccggtt tgcggatctc
    11761 gaactgcgtg gtgtgcaccg ccactgccag cccgcccacc ccagcgtcca gataataccg
    11821 gatcagcgtg cgctggcccg cctcgttgaa tttccggttt tcgtccagaa ccagcgggat
    11881 ggccgggatg accgtgcccc cgtgcaggat cttcagcgct tcttcatgcc gtcccatact
    11941 caatagctcc ctttcctctc ttcaaagtgc gtgggcttgc ccaatgcgcg ccctccgtcc
    12001 aaaatccact gtgcctgcca ttcgatcagc gtttccagcg gcaccgtggg atagccgaac
    12061 agccggaaca tctttcccgc gttgttcaga tacgccgtgt ccgacgcctc ccctgtgaat
    12121 atgggctctc tgcccagcag cgcgcccagc tttttcgccg tctcctgtac gcccgcggtc
    12181 tccggccccg tcacgttcag cgtgaacacc tccgccgagg catgccccag cagacgcagc
    12241 gccgcctcgt tggcgtcccc ctgccagatg cagttgaagc tgggcgtggt gatgctgatg
    12301 ggttttccct ccaggatgtt gtgggccata tcgtacagca cgccgtaccg caggtccacc
    12361 gcgtagttca aacggtacac cgccacccgc gtgccgtagg tctttgccgc gtactcgaac
    12421 atccgctcgc gtccctggca ggacatgcag tactcgccca ccggctccgt ccgcgtctcc
    12481 tccgtggcac ccccgctgtg cacacccacc ttcgggtaca ggttcccgct ggagaacacc
    12541 acgatgcggc tgttttggta ccgctcggcc acccggctgg gcagccaggt gttcatcgcc
    12601 caggtggcgt attcattgcc gtccgtgccg aactttttgc ccgccatgta gatgacgttc
    12661 tccgcgtccg gcaggctgtc cagcgcgccg ggcgccagca ggtccgcgct gatcatctcc
    12721 accccgttct cccgcagcag ctccaccacc agcgggtcgc tgaagcgcga caccgcgatg
    12781 actcgctttt ccactcccgc cgcccgcagc gcgttctttg ccagaacgca cagcgtcggc
    12841 cccatttttc cgcccgcgcc cagcaccagg atgtctccct ccaaccttcc cagctccccg
    12901 ctcagcgcct cgctcggcgt cgtcagcatt ttgttcagtc tttcttccgt ccacatttcc
    12961 gtcgttcctc ctgtatttgg aaaagtcgtt ttccgcgcaa ggccgccaca gccgcccgca
    13021 ccgccggcga aaggacgcgg tgcgggacct gcttttatta tgcaggaccg aggctgcggc
    13081 gtaaagtgac attttttaag aatgcggttt ctattttaca ggccgcccgg agcggagaaa
    13141 agtgtaattt ttgaaagccg gtttaaaaaa tgatagcgcg gcgttttgcc gcgcctgcgc
    13201 ggtatcttta attttgattg cgggggctta atttctggca atttacccgg ccccggacgg
    13261 atgatatgct gaaaacagag caaggaaccg cggcgggaac accacagaac gccgcgccga
    13321 acgcctctcg ggccgcgcca cagcccggag gctgccccgc agcttatata taaacctaat
    13381 ataagaaaag cgggaacggc cgcgccggca aagaggctgg gcggcacaag caaaggagga
    13441 acgacccaac tcatggagac tgaagtaaag acacgccgga aaattcagct gcggatgccc
    13501 gggcggcgga cggccaccgg taaaaatgta aactggattt tgatgaagcg caataaggtg
    13561 ctgtatcttt ttattctgcc ggcatttttg tatttcctga ttttcgccta tgtaccgatg
    13621 tacggtgtgc agatcgcttt caaggatttt gtcgccaaca agggtttttt gggcagtgaa
    13681 tgggcagacc cgctgttcaa gcatttcatt acgtttttca gttcggtgaa tttctgggag
    13741 attctgcgga atacgatcgg gctttctctg tattatctcg ccatcagttt cccgatgccc
    13801 attcttctgg cactgatgat caacgaggtg aagaataccc ggttcaaaaa ggctgtgcag
    13861 aacattactt atatgccgta ctttgtgtcc accgtggtgc tcgtcggcat gatcaatctg
    13921 ttcttttcct acaacggtct tgccaacaat atcggcgcgc tgtttggagc ggagccgcag
    13981 atgttcctga tgaaagatgc gctgttcaac gatttgtatg tttggagcgg cgtgtggcag
    14041 gccacgggtt acggcgcggt catctacatc gccgcgctgg cgggcgtttc gccggacctg
    14101 cacgaggcgg ccaccatcga cggcgctaca cggctgcagc gtatcatcca catcaatcnn
    14161 nnnnnnnnat cgtcatcatg ctaatcatgg ccgtgggcgg catcatgaac ctttcctttg
    14221 aaaaggtgct gctcatgcag acagacagca acactgcggt gtcggaggtc atctccactt
    14281 atgtttacaa gctgggcatt caacgcgccc agtacagcct gtcggccgcc gtgggcctgt
    14341 tcaacaatat catcaacttg attttgcttg tgacggtgaa caaagtctcc cgcaagatca
    14401 gcgaaacatc tttgtggtaa ggaggcgcct gcaatggtta ttaaagagac aagaagcgac
    14461 aagatattca atttcatcaa ctatttcctg cttgcatgcg tgttcctgat ctgtctgtat
    14521 ccgttgtggt tcatcatcat cgcgtctatc agcaacccgg atcttgtcaa cggcggccag
    14581 gtatggttct ggcccaagga tatttccttt gacggctata agagcctgtt cgccgacgac
    14641 ggcgtgatgc gcggttatgt caatacgctg atttatacca caggcgggac gctcatcaac
    14701 cttgccgtta cgctgcccgc tgcttacgcg ctttcccgcc gggattttgt aggccgcaag
    14761 tttttcacgc tgatgttcct gttcacgatg tttttctccg gcggcctgat tcccaccttc
    14821 ctgacggtga aaagcctggg gctgtacaac aatccatggg tcatggtcat tctgggcgcc
    14881 annnnnnnnn nagctgcggg aggctgccga gatcgacggt tgttccaata cgcgcttctt
    14941 tttcagcatc gtgctgccgc tttccacggc tatcatagcc gtcatggcgc tgttctttgc
    15001 ggtgacacac tggaacggtt attttacggc gatgatctac gtgcgcgaca aagactggca
    15061 gccgctgcag atcgttatgc gcgagatact gatgcgcagc gaatttaacg cccagatgct
    15121 ccagcaaggc ggggatacgg gcgtgctggc ggcgcaggaa cagcgcatcg ccgagcagat
    15181 caaatatgcg ctcatcgttg tggcctcggt accggtcatg tgcatttatc cgtttgtgca
    15241 gaagcatttt gtcaaaggtg taacagtggg cgcggtaaag ggatgattcc cttgccgctc
    15301 cgcacgtgat acaattatta aaacaggagg aagcaatatg aaactgaaaa aactggcatc
    15361 ccttgcaatg tccggcgtgc tgtgcgtcag catgctggcg gcctgcggcg gcggttcgtc
    15421 gaccagcgcg gccgtatccg gttcgg
//
LOCUS       DAWBSB010000022        15145 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_994_length_15145_cov_3.591518, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000022 DAWBSB010000000
VERSION     DAWBSB010000022.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 15145)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 15145)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15145
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_994_length_15145_cov_3.591518"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3193 a         4415 c         4597 g         2940 t
ORIGIN      
        1 gctcagggct tttgcgcttt ttttcacgcc gtccaggttt ttggctttcc ggggcgtttc
       61 tgtgccttcg tcccggcttt tgacaaaaaa gaaataaacg gccactgcag cggcgataac
      121 aaggagcaga agcaacagat ccatgggagt caaagtgaaa aacctccaat aaaacaaaaa
      181 taccgcgcag cgcaaggctg cgcgcagtaa aatcctttta caagtatacc aaagcccgcg
      241 ccaaaacaca attcccggcg gcgcacgaaa cgtaaattta ttttgaactg aggaaaaaga
      301 acatgaaaca tgagaaatcc tgcggggcga tctgctatac ccgcgaagaa ggcgcgcccc
      361 gcgtgcttgt catctgccac cgctatggcg gccactgggc ttttccgaaa ggccatgtgg
      421 aagccggtga gagcgaggaa gagaccgcca tgcgcgaggt gcgcgaagaa acgggcgcgc
      481 aggtacgcgt gcgtccgggc taccgcgagg tgacgaccta ttcgcccgca aagggcgtga
      541 caaaggatgt tgtgttcttt gtggccgaga tcaccggcgg cacgctgcgg gcccagccgg
      601 aggaggtgcg ggcagtttgc ctgctgccgc tggaggaagc acagcgccgg ctgacttatg
      661 ccgccgacaa ggaattgctg gaaaaggcaa aaaatatgat tctggacgaa aaaaacggtt
      721 gacaaccgtg ttcgactatg ataacatata tgatgcactc caaaagcgga gtggtatgcg
      781 ctgctagctc agttggatag agtgtttggc tacgaaccaa aaggtcaggg gttcgaatcc
      841 cttgcagcgc accacaggac ggtatcccgg ttgggatgcc gtttttgttt ttcaacgata
      901 ttttgcactg gaaagacact tcagcgtgca gccgtgtcca cggaacccgt tccgcaggaa
      961 cggtgggata tacagttttt tgtcatacac gagtgctata ataaagtgat aacaatgaag
     1021 taaatacaaa agggaggctc gcggatggga tggtttttgc tgattggcgc actggccgtt
     1081 acgttcgttg tttattgctg cctcattgtg gggagcgatt cctttcagga cagacgcaaa
     1141 taggcttgcg gacggcgcag agccgaaaaa tatagaaacc cgtccccggc cggggacggg
     1201 ttttccatat cttcgtttga cgttattgcc tcggggacgc ggtcttccgg cggcgcgctg
     1261 caatgcacag cgttaccacg ggaagccaga aactcggaag cagcatcagg ttgaacagct
     1321 ggaacagcag cgttttccag gacaatacag cggcgcccgc cgcggaggca gcctgccaca
     1381 atgacaatat gcccaacagg agccagcaca gcaaagcggc ggcggaaagc atcaaagcaa
     1441 gcgtatgctt tgcacgcggc ccgttttgcc cccagcgcag cagcacgcag gcggcgcagc
     1501 atcccagaat gaagggaacg gcgagccaca gcggtgtgag caccggggcg agaagatttg
     1561 cagcgcctgt tataaaaatg gccgtcggcc cgtctgcacc gccgataacg cccatgccgg
     1621 agctttgaaa ataaaaaagc agaaagggaa gaatggaaag cagtgctgct ccgcccgcca
     1681 gtatcgccgc aatgaggaac acccagcgcc aggggctgcc tttgggaaga agctgatcct
     1741 gcatttcctc gttaaagccc gcggcaacct cctgcggcgt gcccatctcc gcgatgatct
     1801 catcgatgct ttgtcctttt tccagccggg cgtggatatc ggtgccgatg tccccgttga
     1861 tgcgcgcttt tgctttcaga ggcaggcgca gctcccgctc gatggcattg acatagcgct
     1921 tgatcttttt ctcctgttcc gtcataactg ctcctccctg tcaaatccgt ccacgcagtc
     1981 cgcgaaggcg cgccatgctt tgcgttgtac ggcataagcc gcatgaccct ccggcgtggc
     2041 cgtgtagtat tttttcggtg cggtgcggcc cccgctggta cgccattgcg cttcgataag
     2101 cccatcgtct tccagccggt acaaaatggg atacaacgtg ccttctttca gcgcaaaaag
     2161 cccgttgctg cgccgggcca tgctgcccag cagctcgtag ccgtaagtag gtcccgcaca
     2221 caccagcccc agtaccagca tctccagcgc gccctttttc aactgtcgtt caaagcgcga
     2281 ttccatccgc tcccctccgt cctggtttta tacctagtat tactaaacac taatatagca
     2341 taaactttgg gaaagtcaag accgttctgg aaccggcgcg cgggttttca gccggggccg
     2401 gcgcctgtgc ttttggtgca ggcggtgcag cagagcggga agtgtggtat aataacctca
     2461 agcccgccgc ttacgtggct gcgcgcattt catgcgctga tgcgctttcg cgcatgggct
     2521 tgagaacatt gaaccatgca aaaccgcagc aggcgcggtt ttgttggggt cgcttcgcgg
     2581 cggagatatc tgacttaccg ggagggatac cattgcagct gttcgatgcg catatgcact
     2641 attctgatac ccatggtccg gcctttgacg cggtgcggga gcgccacggc gtcggggcct
     2701 gtacgctggc gtgtattccg cagatgggcg tatgctccac cgtgccggat gcgctttatt
     2761 acaaagcagt gcatccggag ggaactgtct atgtgatggg agggttggaa cgcagcgcct
     2821 ggtttctcca cgagggggat gcggcggcgc tgggcgaaga tctggtcgcg caggccgggg
     2881 cgctgctggc cgcaggctgc gacggcatca agctgctgga gggcaaacct gatatccggc
     2941 gcaattttcc cattccggat ttcgatactc cggcatggga accgttctgg cgctgggcag
     3001 aggaacagcg cgtgcccatt ctgtggcatg tgaacgaccc ggaggagttt tgggacgcct
     3061 cccgcatcaa tccctatgcg aaaagcgagg gctggttcta cggcccggaa accatcaaca
     3121 atgaggagca gtaccgtcag gtgtttgcgg tgctggcatg ccacccgggc ctgaggctcc
     3181 agctggcgca cctgtttttc ttttcggcgc agctgccgcg gctttcggcg ctgctgcggc
     3241 gttacccgga ggtgcgggta gacctgacgc ccggtatcga gctgtacacg aaccttgccg
     3301 gagatattcc tgctgcacgg gcattttttg aagaattcgg cacgcgcatc ctctatggca
     3361 gcgatatcgg ctcccgtgcc tccatcgcgc agccgccccg gccgctggac agcagcgaga
     3421 gcgccgcgcg ggtggacgtg atccgcacgt ttctggaaac gccggagaat gaaccctata
     3481 cgctgctgcc ggacggcaga tatctgttcc gcatcgcacc cacgcccatg cgcggattgg
     3541 gcctttcatc cgaggcgctg gagcaggtat acggcggcaa cgcacgaatg ttcctgggcc
     3601 gtgcgagacc ggtggatgcc gcaaaagccg cgtcgctggc acacagcttt gccgccggcg
     3661 tggaggcgct gcatgcaagg gagctgtttt tggcggcgga tgcggccccg ctgcggcaaa
     3721 aggccgtgca gctgcaggcg ttggcggagt aagcgggcgc ggtccgccgc atactaatac
     3781 agaacatgga aaaggggaga gcgaaatgaa gacagacaaa cgcaaggttg ctctggtagg
     3841 ctgcggcatg gtgggcatga gctatgccta cgctatgctg aaccagaacg tatgcgacga
     3901 gctggtgctc atcgacgtgg acaaaaagcg cgcagagggg gaggccatgg atatgaacca
     3961 cgggctggcc ttttccggca gtaatatgaa gatctttgcg gggagctacg acgattgcgg
     4021 cgatgcggac attgtgtgca tctgcgcagg tgtggcgcaa aagcccggcg aaacgcggct
     4081 ggaccttttg cagcgcaatg ccgccgtctt caaaagcatc attgacccgg tgacggagag
     4141 cggcttcaac gggctgttcc ttgtagctac aaacccggtg gacatcatga cacgcattac
     4201 ctgcgcgctg tcgggcttca atccaaggcg cgtgctgggc acaggaacgg cgctggacac
     4261 cgcgcgtctg cgctatctgt tgggcgatta ttttcatgtg gacccgcgca acatgcacgc
     4321 ctatgtgatg ggtgaacacg gcgattcgga atttgttccc tggagccagg ccatgctggc
     4381 cacaaagcct gtgctggagc tgtgccgcca gtccggcggg cgctattgca gcgaggaact
     4441 ggataagatc gctgaagaag tgcgcacggc cgcctacaag atcatcgaag ccaagcgcgc
     4501 cacctattac ggcatcggca tggcgcttac acgcatcacg cgggcggtgt tcggcaacga
     4561 aagcagcgtg ctgacggtat ccgccatgct gcggggcgaa tacggccaga aagacgtatt
     4621 tgtgggcgta ccctgcattg tgaaccgcag cggcatccag cgtgtgctgg agctgaacct
     4681 ctccgaggag gagcaggcac ggttcagcgc ttcctgcgag acgctgcggg agagctatgc
     4741 cggactgaag ctataaacag aagaaaagaa aaagaagcag acggctctgt gccgtctgct
     4801 tcttttttgg cccggaaggg tcaaagcgcc gcgtcctccg gcgcgcgcag cccaaaaacg
     4861 tcccacagca cctgcgcggt ttctgcgcct ggcgcaacgg agcgggaacg ggccactgcg
     4921 cccttacgat accatgtcag cttgctgtcg cgcagtgaca aatgagcatc cggtaaaaaa
     4981 atagaggccc gcaccgtttt gttgaaaggc gacatcggat gcacgtcgca aaaaaggctg
     5041 ggcataataa agtcgatgtc gagctgcggt tcctcggtaa agccatacag ccggcgccag
     5101 gctccgcccg gcgaccgttt ccacaggacc cagccccaaa aggcgtcgcg ggaaaagcgg
     5161 tattccgtca cgccgtccca ttgcgcttcg ctctcggcca gcacaagcgg cgcgcggggg
     5221 ccttcgctgt tcatgcctgt ttcggtgaga taccgcttcc cctccagcgt tacgacgagc
     5281 acacggtggc ggcgcatctg gatgccggcc tccgaaggat agatgaaacg gcccgcatag
     5341 ctggtgacct ggtatcccag cgaacacagc agccagcaga acaggccgtt cagctcgaag
     5401 cagaggccgc cgcggcgctt ttccacgatt ttttcaaaca atgctccgtg ttccagagcg
     5461 gtgatcgcgc cgcgcaggag gtccaggttt tcatagggca catgcagcag atgtgcggac
     5521 tgcaggcggc gcagcgtttc cagcgtggga ggaggaggga cggcgtcccg caggccgatg
     5581 cggtcaagat agcgcaggat ctgttcgggc ggcatcggtg tttcggagag cagcgcgtcc
     5641 tccgcgcaga tgggtcggtt cggcattgta aaagctcctt tgatgatttt ccgcagcact
     5701 cataccgaga gcgccaggca cagcatgccc agataatagg cagccagatt caccgcacgc
     5761 agcgctttgc gcggcctgac ataaaaataa acgaacagca gcaccgcgtc cgaaatgagg
     5821 aacagcgcgg cccccacggc agccagcgcg ccgcgccgtc cgccgcaggc aaagccgaac
     5881 gctgtgccgc gggcgcacat cagccctacg cagagcgagt acgccaaagc gggcagctcc
     5941 atgtgcatgc gccgcagctt gaagcgtttg tgccgcgcgc tggcagccgt cacggtcaac
     6001 gcggccagcg gagcggcggc gtcccacagt gtgaagcgcg gtccgcccgg catggccagc
     6061 gtgaacagca cgcagaaccc tgcgtgggcc agtgtgaaag cgcccactcc cagcaggaac
     6121 cagcggccga aaaagcccgc gtggttattg gccagcccca gcagcacgtc tccggccagg
     6181 cacagcacaa agcagggcag aagcccccgg aaatacgcat ctgccgtgtg tccgcccagc
     6241 aggtacgccg tggcggctat gacaaggaac ccgcagctta cggcggcctt taccgccgta
     6301 tagcggttga aatggcggcg ggaatatgcc acgcagagca gcgcggccag cccgcccaaa
     6361 tagatgaggg aaagcagcgt cagcacagcg gcagccatag ccgcctcctt cccggccctc
     6421 aggccgtgtt ttttgcatga aaacgaaaaa cgccgggcgc gggtttcaaa ccggcccggc
     6481 gtttttgtgt cacatgcttt cgggcacggt gatcttcagc agggtaagcg cgttggagat
     6541 gacgtttttc gtagcggtgc acagtgcaat gcgcgcctgg caaacgcagt gctcggcgtc
     6601 ctttacgcgg caggaattgt agaatttgtg gtagcggttg gccagctcgg tgacgtagcg
     6661 cgtgatgcgc gccgggtcat agcttttggc cgcggaaatg atttcgccgg gaaagtctgc
     6721 gatatggcgg atcagttcct tttccgcggg ctctgtcagc agcgtcagat ccgccttgtc
     6781 cgcgccctcg aacacgatgc cgtcttcggc cagctttttc aggatgctgc agatgcgggc
     6841 atgggcgtac tgcacataat acaccggatt ttcgctgtcc tgcgccacgg ccaggtccat
     6901 atcaaagtcc attacgctgc ccggctcccg caggttgaac tggaagcggg cggagtcgat
     6961 ggggacctcg tccagaagct ccgccagccc gatggctttt ccggtgcgct tgctcatgcg
     7021 caccggctgg ccgtcctgca tcaaacgcac gaactgcatc agcacgatgt ccagcttttc
     7081 gccgtctagg ccgatggcgt ccatggcgcc tttcattcgg gccacgtggc cgtgatggtc
     7141 tgcgccccat acgtcgatgg cgcgggagaa gccgcgcacc gcgaatttgt tgtagtggta
     7201 agcgatatcc gccgcaaaat aggtgggcac gccgttggcg cgcaccagga cttcgtcctt
     7261 atcctcgtcc gtcacttcgc cgtccaggcc cttgcgcgtc accttgttgc cgccgtattt
     7321 ctcggcgtac tgcatggaac ggtaccagat ggcgccgtct ttttcgtagg tggcgccgcg
     7381 ttcggccaat ttgtccagca tttcccggat ggcgccggat tcgtgcagcg tggtctcgct
     7441 gaaccagacg tcgtaattca cgcggtattt ggccatgtcg tctttaagcc cctgcacgtt
     7501 caacggcagc gcaaaagccg cgatatcctt ctgcagttcc tcaaaaggct tttccatata
     7561 gacgttcccg tgtacgtctg caaatttctg tgccagcaca cggatatccg caccctgata
     7621 gcagtcctcg gggaaaggta cggcgtcctc gcccctgaag atctgaaggt agcggacggc
     7681 aaggctcttg ccgaacttga ggatctggtt gcccgcgtcg ttgatgagaa actcccgtgt
     7741 gacgtcatag cccgcccagt ccatggcggc ggcgaggcag tcgcccaaag cgccgccgcg
     7801 ggcgttgccc agatgcatcg gacccgtagg attggccgaa acgaactcca cattcacctt
     7861 ttcacctttg ccgtagcttg tgcggccgta gtccgcgccg gctgcgttgg cggctaaaac
     7921 tacgtcggca aaccagcccg gggcaaggaa caggttgatg aagccggggc ccgcgatctc
     7981 cacgcggctg aaatgcgttt cctccaaagc gggcatcgca gcggccacgg cctcggcgat
     8041 tttacgcggg ggcataccga atacgcgcgc gcccgccatg gcgaagttgg aagccaggtc
     8101 tccgttcttc acgtcgttcg gcacctccac cacaaaggcg ggcagctcct tttccggcag
     8161 gctgccgtcc gccatagcgg cgcgggcagc ggcctccagc agcccgcggg cctcgtccat
     8221 agcggcctgt ttcgggtcga aatgtttaaa atcctgcatt ttttcaaaac tcctgtccct
     8281 ttgaattcct gttataccgg cgcgccccgt cagttggcgg gcttgacggt gatctttaca
     8341 atgttgcggc tcacggcggc ggtttccatg tccagcagat agctgaagcg gacggtgccg
     8401 ccctcgtcgg aaaggcggct gtggatctcg tccgcggcca cgcccaggct cagcgaacca
     8461 tggcccgtct cgtaatggca cacatgccgc cggccctttt ctatgacgag ctggctgggc
     8521 gccgggccga agcgcagcat gctcaccttg tgtgaatcct ccacctttac ggtggtcaca
     8581 cagcctgcat agcctgtggc ctcggtttct ttgtagctga tgaagaagct gccgttgcgc
     8641 ttgacaaagc tgccccgcgt catcagcttc acgctgtcgc tttcgccgtc ctgctccatg
     8701 gtgccgtgga tggtgatgag ataatcttcc ttcatacagg gtcatccttt tcctttttac
     8761 gttgaggcgc cttgctccgc gggataaccg tccacgctga tgcgcgtcac gggcccgccc
     8821 gcgtattcgc ccaaaaacca gtccgaaagc tgggcgaagc tctcggtggt gtcgcttaca
     8881 taatagcgtg ccgtgcccgc gcggccctcc cgcagcagcc cggcggaggc cagcgcgtcc
     8941 cgtgcggcca gcgcggtttc ccgcccgggg tcgatgagcg ccacttcggg gcccatcaga
     9001 tccgcgatga tgggcgcgat gagcgggtag tgcgtgcagc ccaggatcag cgtatccacg
     9061 cccgcggccc ttatgtcctt caggtaatcc cgtgccacaa ggcgcgtcac ggcgttgtct
     9121 gcgccgaaat acccgttttc caccagcggc acgaacagcg ggcatgcggt ggctacgatg
     9181 cgcgcgccgg gcatcagcgc ggcgatggcc gcgttgtagc tgccgctctt aacggtggcg
     9241 ggcgtaccga tgacaccgat gagccctgtt ctggtggcgc gtacggcggc ggccgccgcg
     9301 ctctgcacca cgcccgtata aggcacgggc agcgtgcgtg tgtaagcctc ggacagcgtg
     9361 ctgctcacgg tgccgcaggc ggccatcaca tatttcacgt tttgctccag aaggaatgcg
     9421 atgtcctgct ttgcgtaccg tacaatgaca tctacgccgc gggtgccgta gggcacccgg
     9481 cccgtatcgc cgaaatagac gatgtcctcg ccgggcagga tgcgccgcag ctcgcgcacg
     9541 gccgtcagcc cgcccaggcc ggaatcgaac acgccgatgg gagcgccgcg ccccggcgcg
     9601 tttcgctcag tcatggacgc cgcccgccct ttccagcgcc agcgtgatca ggctgtccag
     9661 caggccggga tacgtctcgc ccgccgccag catcagcttg ggatacatgc tgatgctggt
     9721 aaagcccggc aaggtgttga tctcgttgag cagcacgcgc tgtgtgccgt gctccacaaa
     9781 aaagtccacg cgggccaagc ccttgcagca cagcgcccgg taggcgcgca cggccaggtc
     9841 ccgcacctcg tccagctttt ccggcgcaag ctgcgccggg atggccaccc ggctggtgcc
     9901 ggagatgtat ttgtcatcgt aggtgtaaaa ttcggcgctg gccaaaattt cgccgggatg
     9961 ggtgccggag acttcgtcgt tgccgcgcac cgcgcattct acttcctgcc cctgtacgaa
    10021 ccgctcgaac accacgcggg aatcgtgctg cagggcgagg tccacggccg cctccagctc
    10081 cgccatgtcc cgcgccttgg agatgcccac gctggagccc gcgttggcgg gcttgacgaa
    10141 aatgggccag cccagttttt ccgccgcgcg ccgggcaatg gccgccagct ccggccgctg
    10201 ccagcgctcc atgcagtccc attcacagcg gggaatgccc gttgcatcca tcacacggtt
    10261 ggcaatgatc ttatccatgc acaccgcgct gcccagcacg ccgcagccca catagggcag
    10321 gccggccagc tccagcaggc cctgtatcgt gccgtcctcg ccgtttttgc cgtgcagaac
    10381 gggaaacacc acgtccacgg gcaggctgcg gcagacgccg ttttcaaacg ccacaagccc
    10441 cgccagcccg ctgcccgggc agatggctgc gggcacattg ccggagtggc gctcccaggc
    10501 gccgctgcaa aggtcctcca cagggccgga gtacaaatac cagcgcccgt ctttcgtgat
    10561 gcccacgggc agcacatcgt atttttccgt gtccagattg ttcagcacgc tggtcgcgct
    10621 catgcgcgat acctcgtgct cgctggacgc gccgccgaac agtacggcga ctttcaattt
    10681 cttcaaaaca ctccacacct ttcctcgggc cgccgtggaa taaagccctg gggcaggcgc
    10741 ctgaaaaccc gcgtgcacaa aacgccctgc tttcagaccg cccggagaaa ggctttcggg
    10801 acggaccgct gcgcggcagc acggctgttt tgccgtgctg cccgcatggt atcacttgta
    10861 tgctgcaatc gtagccttta taataccata caatggttga ttttaaaaga cggcgcggca
    10921 aattttgacg cgcggcggaa aaagcccgga aaacggcccc gcaggcgaaa aaatcccttg
    10981 acgcgggact gtgtgctgtg ctatgatggt tgtacctctt ttgcaggtct atttttttgt
    11041 gcgcattttt cgatgcgccg cctgcggcaa gagggaggct atttgatgga ggtgccgaaa
    11101 aatgagagtg aagatcaccc ttgcctgcac ggagtgcaaa cagcgcaact acaacaccaa
    11161 gaagaacaag aagaacagtc ccgacaggct cgagatgaag aagtattgcc gcttctgcaa
    11221 aaagcatacc gttcaccgcg agacgaagta agaaaggcgg acatgcaaca tggctgacaa
    11281 aaccgagaaa aagtccaaag gctttttcgc tggcgtgaag cgcttcttcg gacgcatcgg
    11341 caagtttttc cgcgataccg tgagcgagat gaagaaggtc gtatggccct cgaaaaagca
    11401 gatcatcaac aaccccgtcg tggtggctgt cgtggtggtc gtggccgcgg tactcatcat
    11461 ggtcctggac gctgtgttcg gcttcgggct gaaccagttg ctgaagggcg tgtgaccgtc
    11521 cgcactttta aagcagaaag cggggttttg acaaatggca gaggatagag acaccgaggc
    11581 gcgctggtat gtggtgcaca cctactccgg ctacgaaaac aaggttgcgc aaaaccttga
    11641 aaccatcgtg gagaaccggc gcctgcagga gcttatccat gaggtaaagg tccccgtgga
    11701 gctggtgccc gacatcaagg acggcaagga gcgcgaggtg gagcgcaagc tcttcccggg
    11761 ttatgtgctc gtcaaaatgg tgatgacgga cgacagctgg tatatcgtgc gcaatacgcg
    11821 cggcgtcacg ggctttgtcg gcccgtcgtc caagcccatc cccctcaccg acgaggaagt
    11881 ggataagctg ggcgtggacg tacgcgaagt gagcctcgat tacgcggtgg gcgacaacgt
    11941 tcagatcatg aacggcccgc tggaaggctt catcggtatc gtggagggca tcgataccga
    12001 cgcaaaaaag gtaaacgtaa aggtttccat gttcggccgc gaaacgccgg cggagctgga
    12061 ctttacacag gtgaaacccc tgtaagccct gaaacgtttt tccggtaaga gcacgcgctg
    12121 caaggcgctg tttcttatag agcgcgcgaa gggtatcgcg cgttcgtggg agggccttgg
    12181 caatccggcc aaagcccgca acttaccaca atttttggag gtgcacaaaa tggctcagaa
    12241 agtaacgggg tatatcaaac tccagattcc ggccggcaaa gctaccccgg ctccccctgt
    12301 tggccctgcg cttggccagc acggcgtgaa catcatggcg ttcacgaagg aattcaacga
    12361 gcgcacgcag aaggatatcg gttacctgat tcctgtcgtc attacggtct atgcggaccg
    12421 ttccttcagc ttcatcacca agacgccccc ggcggctgtc cttatcaaga aggctgtcgg
    12481 tatcgagagc ggttccggcg tgcccaacaa gacgaaggtc gcgtccatca cgaagaagca
    12541 gctggaggag atcgccagca ccaaaatgcc cgacctgaac gcggcgaacc tggagacggc
    12601 catcagcatg atcgccggca cctgccgcag catgggcgtt acggtcaccg actaactgtc
    12661 cgcataacaa ccttaccatt agtgggagga tcattccgta ttaccacagg aggatattat
    12721 gaatcacggt aaacattatg tcgacagcaa aaagcttgtc gaccgtacaa aactgtacga
    12781 caccgaagag gccctggcgc tgtgctgcca gaccgccaag gcgaagttcg acgaaacggt
    12841 cgagctgcat gtgcgcctgg gcgttgacag ccgtcacgcg gaccagcagg tgcgcggcgc
    12901 cgtcgtgctg cccaacggca ccggcaagga cgtgcgcgtt atcgccatcg ccaagggcga
    12961 cgccgccaag gccgctgaag aggcgggcgc gatgatggtc ggcgacgccg atctggtcgc
    13021 aaaaatccag aacgaaggct ttattgattt tgacgtgctg gtcaccacgc ccgacatgat
    13081 gggcctggtg ggccgtttgg gtaaggtgct gggcccccgc ggcctgatgc cgaaccccaa
    13141 ggccggtacg gtcacgcccg atgtgggccg cgccgttacc gaggcgaagg ccggtaagat
    13201 cgagtaccgc ctggacaaag ccaacatcat ccatgtgccc atcggcaagg cgtctttcgg
    13261 cgccgagaag ctgggccaga acctgtccgt tgtgatggaa gccatcgtca aggcgaagcc
    13321 cgccgcggcg aaaggccagt atatcaagag cgctaccgtg gcgtccacga tgggccccgg
    13381 catcaagctg aacagcgcaa aattcggcgg ctgagctgca aactgaaaaa gcgcgctcct
    13441 gctgattttc aacatcggca gcaaaaagcg cgcgaacgcg cggctttggc aacacggccg
    13501 gcgcgtatct tccggcgcat ctacgcggaa aagcgcggaa cggttttatg cgaataaaac
    13561 cgcagcgttt ccggcttttc gggtttcgct gcgcaagctg cgggaaatcg acgggaacgc
    13621 cgggagaagc acagagtgaa tttgcccctt gacgcgcgca aaactctgtg ttaaaataat
    13681 aaagctgttt taaagacagc aggtgctttt gcataaaggg tttcgcccgc ctgccgagaa
    13741 acgcgaaaaa acatttaatt gtattttttt gcctctttct cgcaagggaa agaggctttt
    13801 ttatgtgctt acacacaatt gaaacgaggt gaaatgattt gccaagtgag aaaattttga
    13861 gcgagaaaca ggcttatgtt gccgccctgc gtgaaaagct gatgaattct gttgccggcg
    13921 ttgtcgtcga atataagggt atcagcgttg cggacgacac caaactgcgc gccgaactgc
    13981 gcgcggcagg cgttgagtat gccgttgtga aaaacaccat gctgcgcctg gccattaagg
    14041 actccgacct cgccgggatg acggacgccc tgacgggttc caccgccctc gcgatctcga
    14101 aagaggatcc cattgctgcc gcccgtattc tgaacaagtt tgcggaaggc tccaagaagg
    14161 gcttcgccct gaaggccggc tacatggaag gccaggtcat ggatgttgac gccgtcagcg
    14221 ccatcgccaa gctgcccgac cgtgaaggca tgctgtccat gttcgcaggc gcccttacca
    14281 gcaccctcag cggtctggct gtggccatgc aggcttatgt ggataagcag gaagaaccag
    14341 cggcataatt tcggccgcac ccaaacacaa aacaacacta aaataaaatt ggaggtatta
    14401 tcatggcttc tgagaaaatc actgccatta tcgacgagat caaaggcctg tccatcctgg
    14461 agctgaaaga gctcatcgac acctattgcg aagaattcgg cgtttccgct gttgccgctg
    14521 ctccggctgc cgccgctgcc gctgctcccg ccgccgagga gaagacggag tttgacgttg
    14581 tgctggctga agtcggcgcc aacaagatgg ccgtcatcaa gaccgttaag gaagttaccg
    14641 gtctgggcct gaaggaagcg aaggacctgg tcgacaacgc tccgaagacg ctgaaggaag
    14701 ctgttgcgaa ggacgctgcc gaggaaatga agaagaagct ggaagatgcc ggcgccaaga
    14761 tcgaactgaa ataagtttgg tttttccaag cccccgctgc aggacgtagc gggggctttt
    14821 ctgatttatg gagtttgtga aaatataaat ttaatattga ctttaataaa attaaagtat
    14881 atatttaata tagtgaaagg aggcgcgcta tggcaattga aacggttccg gagtggatgg
    14941 ccgggctgga ggatgaggac gtggcgttca tcaaaaaatt cctgctggcc tccggcagcc
    15001 tgaaggaagt ggccgggctg tacggggtca cttatcccac ggtgcgcctg aggctggaca
    15061 ggctcatcca aaaaatacat ttgagcgaag atacagcggc agacccgtat gtggcgcgcg
    15121 tcaagaggct ggccgtggcg gacaa
//
LOCUS       DAWBSB010000023        15061 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1003_length_15061_cov_3.489071, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000023 DAWBSB010000000
VERSION     DAWBSB010000023.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 15061)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 15061)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15061
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1003_length_15061_cov_3.489071"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1007..1016
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5008..5017
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3101 a         4786 c         4211 g         2943 t
ORIGIN      
        1 acattcactc ataaaagtac tccttcgatt tcaatgtttt aaccaaaaac aaaaagccga
       61 cacctataac aaggcatcgg accgtttcgt tgatgatttt atattagcct gtggatgtgc
      121 tgttgcattg gctctttttg ttgttacttc atcatccact gacaccattt ctacaccatt
      181 tcggcgtgaa gatatatgga gaggcatgga gatttaaatt gttgagcacc ttgaaaaccg
      241 catagctatg cggttttatg gacattcatg gagaattgga gatttatgaa aattaggctc
      301 gaaattccga ttccaagctt catataaaac cttcctcttc tcaagcgggg cggcagcccc
      361 gggtatatct ttgattattc taccatacag gcagccctgc gcgcaatgcg ctggacgcgg
      421 agcaccccaa aaaactgcgc gggagctccc gcaggacttt ttttatacac aattatatcg
      481 tataatattg tcaaactgct gtttttctgc gccccgcaga gcatctgcgg tacaccgcat
      541 acggcatgcc gcgcaaaaac gggcgcggta caggggaata aaggagtgtg acccatggcg
      601 aacgagaaaa ttctgatcgc ggacgacgac aaaaatatct gtgagctgct gcgcctgtac
      661 ctggccaagg agggctatga gacggtgatc gccaacgatg gcgaggccgc cgtggccgcg
      721 tttgaaaagg aaaagcccaa catggtgctg ctggacgtga tgatgcccaa aatggacggc
      781 tgggaggtgt gccgccgcat ccgcgcggcg gacaacacac ccgttatcat gctcacggct
      841 aaaggcgaga ccttcgacaa ggtgctcggc ctggagctgg gtgcggacga ctatgtagta
      901 aagccgttcg actccaagga agtggtggcg cgcatcaaag ccgtgctgcg ccgctgcacg
      961 cccgccgagg cccagcccga cggcgtcatc agctatgata agctctnnnn nnnnnnccca
     1021 aggaactgga gcttttgtat tatctggcca gccatcccaa ccgggtattc acccgcgacc
     1081 agctgctgga cgaggtatgg ggttttgaat actacggcga ttcccgcacc atcgacgtac
     1141 acgtgaagcg tctgcgcgaa aagcttgaaa acaccagcga ccagtggagt ttaaagaccg
     1201 tctggggcgt gggctataaa tttgaagtga aagaatgacg gcgggccggg gcccgcggca
     1261 tgccggaaga aaggggggtg cgcccgtgcg caaaagcatc accacgcggt atttttattc
     1321 cacggcagtc ctgctcatct gcagcatcgc cgtgatgggc tttattcaga tgtatctggc
     1381 catgggctac ttccgcgaag aaaacggcac cgccctaatc cagaccatcg acaacgccat
     1441 cctcgccatg caggaggccg cggatgacgg catcgttgaa aaaagggccg aagccgccga
     1501 ggccgccggg ctggatgtgg aggatatcaa aaggggcatc acgctgacgg cccgcgccgc
     1561 cggaaagagc atccttgtga cggacgccta cggctccgtg gtgctttcca tcccgtcgga
     1621 atatcagggc atggaagtgt ctgtaaaaac gtgcaatgcc acatacagcc ggggcattta
     1681 cgacgaaatg ggcactttgg gcggtatctt cagcagccga tattacatcg cgggcaagcc
     1741 cattctcgac gagaaaggca tgctccaggg ctttgtgttc gcgggggcaa acgccgacag
     1801 cctcacggaa tacctggcaa acctgttctc tacgttcatc ctctccgccg ggctgatgct
     1861 gatggtatcc agcgtgcttt ccatcgtgct gaccaaccgc atgaccacgc cgctgcgccg
     1921 catttcggac gccgcgaaaa agttcggcgg cggagatttt tccgcccgcg tgcccgtgga
     1981 gggcgacgat gagctggccc agctggccga gacctttaac accatggccg gcagcctgga
     2041 aaaaatcgac cagtcccgcc gcagcttcat gggcaatatc gctcacgagc tgcgcacgcc
     2101 catgaccacc atcaagggct ttatcgacgg catgctggac ggcaccatcc cgcccgaaaa
     2161 ccagaaccac tacctgggca ttgtctctca ggaggtgggc cgcctgacgc gtctcatcaa
     2221 gaacatgctg gacatcacca agctggaggc cggggagtat aaagtaaacg ccactaccta
     2281 tgatatctgg gagagcatca ccagcgtggt gttcggcgcg gaacagcggc tggaggccaa
     2341 caacatccaa atcagcggct tcgccccaac caagaccttg gttctggcgg atcaggatct
     2401 ggtgtaccag gtcatctaca atctggtgga caacgctatc aagttctgca atgtgggcgg
     2461 ggagatccgt ttttccgtca cacagagcaa gggcgccgtt accgtaggcg tgcgcaacac
     2521 aggcgcgggt attgcgcccg aggcgctgcc ttatgtgttc gaccgctttt acaaggaaga
     2581 caaaagccgt gggctgaaca ccaccggaag cggcctgggt ctgcatatct gcaaagtcct
     2641 catcaatttg tccggcggca agatctgggt ggacagcaag gagggcgaat actgcgagtt
     2701 cctgttcacg ttgcccgcgc cgccttcttc gcccattaag ggcggcaagc tcaaggatca
     2761 gtgaccaccg cgccgcatga ggcatggaag agaaatattc gcggccgcag tgctgcgcat
     2821 tgccgggagg cccggcaggg agagagggaa tcatggatca gcgccagtat ccgcaatatc
     2881 cgcagcagcc cggcgggccc tgcccgccct atgtacaaca gcccgggatg gagtatccgc
     2941 cttatccgcc ctatggcgga cagggctccg cagccccgcc ctacggcggc tccgtttacg
     3001 ggcagggcgg cggccccgtg ccgccgcggg cgccccaata tgtatatcag acgcccggct
     3061 cgatgccggg cgtccccggc gggaaaccgc cccgccggcg caggaaccgt gcgccgctgg
     3121 tggctgttct gctttgcctg gggcttgccg caaccgtttt tctggcggga gaggcttttg
     3181 acgcactgcg cagcggcggc gccgcgcgcc ggcgtcccac gcccggagag gtgcccagct
     3241 ttaccatcga ggatcttccc gaggacgcgg acggcgggct ctccacggtg gagattgcca
     3301 agaccgttgg ccccagcgtg gtatgcatca atgtatatga gccgggcagc atttccattg
     3361 caggcagcgg cagcggcatc attctcaacg aggacggctt catcgtcacc aacgcccatg
     3421 tggtggaggg ctcctccgca gttaccgtgc tgctggacga cggccgtgag ctggacgcat
     3481 ggatcgtcgg ctccgacacc cgtacggacc tggccgtggt caaaatcacg gcggacgggc
     3541 tggtgcccgc ctccttcgga gattcggaca tcctgcaggt gggcgagcgg gccgtggcga
     3601 tcggcaatgc ggccggacag ctctccggca ccgtcaccca gggcatcatc tccggcctca
     3661 atcgtgagat cagcatgcag gcgggcaaca gcgtcgtcac gatgaacctt atccagacct
     3721 ccgcggccat caaccccggc aattccggcg gcgcgctggt gaaccgcttc ggtcaggtga
     3781 tcggcatcaa ttccgccaag ctcaacagct ccatgtttga gggcatcggc tttgccatcc
     3841 cctcggcgga cgcgcagccc gtggtggagg atctgattgc ctacggctat gtaaaggacc
     3901 gcgtggcact gggcgtgatg gtaatcgcgc tgtcgcccgt cacaggccct gccaacgacc
     3961 tgcccagcca gggcttatat atttcatctg tggaagatta cagcgacctg aacaaccacg
     4021 acatcaccgt gggcgacgtg atattgacgg cggacggcgt aacgctggaa accacgtccg
     4081 atctgctgga tgtgctggag acgcacaagc ccggcgaaac cgtgcggctg gagatccaaa
     4141 agcgcggcag cggcaatgtc gtcaccgtgg acgcggtgct ggccgagagc cgcagcaact
     4201 gaatcttcaa aacaaaaagc gccgccgcgg atatatcccg cggcggcgcc tctttttgtt
     4261 tttctcctca ggtgaagaat ttgtccttat agcccatcaa aaagatgaac agcacgatga
     4321 gcggcagaac gtaactgacg tatacccgcg cccattttgg aaaccgtatg cccttgccct
     4381 cgtctgcctc ggccacaaaa tttttccatc cccagccgta gcgggacgtg cagaacaaca
     4441 ggtatacaag actgcccagc gggaggatcg tattggaaac aaggaagtcc tccaggtcca
     4501 gcacggcgct gccctcgccg aagggcataa agcccgaaag cacgttgtat cccagtacac
     4561 agggcagcga cagcacaaga atggcgaaaa aatttaccaa cacggctttt ttgcggctcc
     4621 agccccacaa gtccataccg aaggcaacga tgttttcaaa cacggcaata acggtggaca
     4681 gcgccgcaaa gctcatgaac acaaaaaaca gcgcgcccca tacccggccg cccgccatgc
     4741 cgccgaagat attgggcagc gtgacgaata caaggcctgg gccctgcccc gggttgacgc
     4801 cgaaggaaaa gcaggcggga aaaatgataa ggcccgccat cagcgccaca aaggtatcca
     4861 gcagcgtgat gttgacgctc tcgctgagca gcgtgcgctc cttgccgatg tagctgccga
     4921 aaatggccat ggcgccgatg cccagagaca gcgtgaaaaa ggcctgtccc atggccgcgt
     4981 acaccacatt gcccacgccc tgctccannn nnnnnnncca caaggcgccc gaaatccggt
     5041 acaagataaa atttgaggcc ttcgtccgcg cccggcagtg tgacgctgcg cacagccagc
     5101 gccaccatca gcagcagcag gcagaccatc atggcttttg tgattttctc cacaccgttc
     5161 tgcaagccca ggctgcacac gccgaagccc agcaccacag ccacaatcat ccagaatatc
     5221 tgcggccccg gctgcgccag catgtcattg aaaatgccgc ccaccgcctc ggcggacgcg
     5281 cctgtgaacg caccgcgcag catttttacc acataggcca gcatccagcc cgccaccgtg
     5341 gtgtagaaca tcatcagcag atagttgccc gccatgccca gccaggcgta ccaatgccat
     5401 ttggtgcccg caggctccag cacgcggaag ctgaccgcgc agcttttgcg ggacgcccgg
     5461 cccacggcga actccatggc catgatgggc aagcccagca cgaccaggaa aaacaggtaa
     5521 ataagcacga acgccgcgcc gccgtactgt ccggtaatat aggggaagcg ccatacgttg
     5581 ccgatgccga tggcacaccc ggccgaaatg agcagaaagc ccagccgcga ggcaaatttt
     5641 tctcgttttt ccataaccct gatccctctt tttccttctc cgatacccgg cgcatccgca
     5701 tagagcgccg tctttgtcca gcatagcaaa caaaacgtgc gtgtgtcaac gaaacacgcg
     5761 cacgtttttg cattttctct gttttttccg gccctcagcc gccggaaaga tgctcaacga
     5821 ggatctttgc gccgatgcac accaaaatga cgccgccgaa tatctcggcc ttaccgccca
     5881 gccagctgcc gaattttttg cccagcagat ggcccgcaag gcagcagacg aacgtgacgc
     5941 aggcgatgaa ggaagccgcc gcggcgatgt tcacatccag cgcggcaaag ctgacgccga
     6001 cagccagcgc atcgatgctg gtggcgaagg cctgcaccac catcagctta tagctgaacg
     6061 cgcggcccac ggggcacgcc tccggcgcgc gcagctcctt gacggcgtcc cagatcattt
     6121 tgccgccgat gaagcccagc agtgcaaagg cgatccagtg gtcaaacgcg ctcaccacat
     6181 cggcgaaggc gcggcccgca aaatacccgg ccacgggcat caggccctga aacacgccga
     6241 aggacagcga cgtgagggcg gcctcgttcc gccgcaggcc ggaaaaacac atgctgttgg
     6301 agatggacac cgcaaatgcg tccatcgaaa gccccagcgc gagtacgaac agggtgatcg
     6361 aatccataac gctttttcct ccaaatctgc tttcataaga atatgcgtgt ctgcggccgg
     6421 ataatctgtt ccgcccgcag cgcgcgccgg aaaagggagg cgtccgtttt gcggccctgt
     6481 ggggcggggc cgcaaaaaaa agacccacgg acggcgcaaa gccgcccatg agtcttgttc
     6541 attaagcaaa gccaggcgcg tgccggcgcg ccatcatgtt gactttacac gccgtacagg
     6601 cccggcgcga actactccct caaaggtata agcatatctt accataccct tttgcggcgc
     6661 gcaagtgttt tttcgtccgg cgcgcctttt tcacgcaagg tccggcagtt ctcgccgcac
     6721 cgcgtcctcc agccggcaca gcgctgcggc gtccatctcc ggtgtgtccg caagagggaa
     6781 cggaacgcct atagccccgt atttttccag acacagcttt cggaagccca acagctccac
     6841 cttctgcaaa ttatggaact gtccggccat gcgcgccaat gcgcggatat gtgcctcgcc
     6901 gtccgtcaag ccggggatca ccacgtgccg cacccacagc ggcacgccca gctcttgtgt
     6961 cagcgccaga aaatccagcg tatgggccag gctgccgccc gtatgggcgc ggtatccatc
     7021 ttcggagctg aacttcaggt ccgccagcac caggtccgta tgcgccagca cctcccgcgc
     7081 ggcggcaaga gcacccgcgc cggaggtatc cagcgccgtg tggtagccct ccgcccgcag
     7141 cgccttcagc agcgccgcgg tgaacacggg ctgggccagc ggctcaccgc ccgtaaccgt
     7201 aacgcccccc gactcataaa tgtacggctt acagcggcgc acacgccgca gcacatcctc
     7261 caccgtgtat tccgtgccgc ccgccgcgtt ccaggtgtcc gggttgtggc agcaggcaca
     7321 gcgcagcggg cagccctgca gaaacaccac atagcgcagc cccggtccgt ccaccgctcc
     7381 catggacaaa aacgagtgta tgcgcccttt cacgccgttt tccggcgtct gcatctgtgc
     7441 gtccacggct cacatcgcct cgtggaacgt gcgggcgatg acctcgcgct gctgttcttt
     7501 cgtcagcttg tggaagttca ccgcgtagcc tgaaacgcgg atggtcagat tggggtattt
     7561 ttccgggtca ttgtacgcct ccaccagcgt ttcacggctg agcacgttga cgttgatgtg
     7621 gtgggccatc tgggaaaaat agccgtccag caggctgacg agattggccc ggcgctgttc
     7681 gtccgtcctg cccagcgctc ccggcacgat ggaaaacgta ttggagatgc cgtccttgca
     7741 gtaccggtac gacagctttg ccacagagtt cagcgaggcc agcgcgccgc ttttttcccg
     7801 gccgtgcatg gggttcgcgc ccggggcaaa gggctgcccc gctttgcggc cgtcgggcgt
     7861 ggagcctgtt tttttaccgt acaccacgtt ggaggtgatc gtcagcaccg agagcgtggc
     7921 ttcggctccg cgatacagcg catggcctgc cagctcgttg taaaacgctt cggcctgttc
     7981 gcaggcaatg gcgtccacgc ggtcgtcatc gttgccgaag gcggggtagt ctccctccac
     8041 ctcgaaatcc gtgatgaggc ctgttccggc gtcgcggatg cagcgcacct tcgcgtattt
     8101 aatggccgag agtgaatctg ccagcacgga caggcccgcc ataccgaaag ccatgtagcg
     8161 gtgcacatgg gtgtcgtgca gagccatctg cgttttttca taagcatatt tatcgtgcat
     8221 gtaatggatg acgttcatgg tatcggcata cagttccgcc agccaggggc ggtatgcgtc
     8281 cagcagcgcc gttaccctgc cgtactccag atactcgccc tcatagggcg tatgcaccgg
     8341 gccgacctgc atgttcttca tctcgtcccg gccaccgttg agggacagga gcagcagctt
     8401 tggcaaattg acccgcgcgc cgaaaaactg catgtcttta cccagccgca tggctgagac
     8461 acagcatgcg atggcgtaat cgtcgccgta aatggggcgc atcaggtcgt cgttttcgta
     8521 ttggatggcg tctgtctcgc aggagacgcg ggcgcagtat gtcttaaacg cggcgggcag
     8581 cgcactgctc cacaaaatgg tcaggttcgg ttccggcgac gcccccagat tgtaaagcgt
     8641 atgcagcatg cggaaggagg ttttcgtcac cagcgtgcgg ccatccgagc ccatgccccc
     8701 cacggcttcg gtgatccaca tggggtcgcc gccgaacagc gcgttgtact ccggcgtacg
     8761 caggtggcgc gccatacgca gcttcatcac aaagtcgtcc atcagctcct gcgcgcccgc
     8821 ctcgtccagc ctgcccgcgg ccaggtcccg ctcgaaatag atatccagaa acgtgctcac
     8881 gcggcccagg ctcatggccg cgccgttctg ctccttgatg gccccaaggt agccgaagta
     8941 ggtccactgc acggcctcgc gggcactttg cgccgggcgg ctgatgtcgc agccgtacag
     9001 cgcggccata tcccgcagtt tgcccagaaa gtcgatctgc cgccagacct cctcgatgag
     9061 acggatgttc tcgtccgtca tcgcgccgcg gcccagctcc gccttgtcct ttttcttttc
     9121 ctcgatcagt cggtctgtcc cgtagagcgc cacgcggcgg taatcgccga tgatccggcc
     9181 ccggccatat gcgtcgggca ggcccgtgat caggccgcag tgccgggccg cacgggtctg
     9241 ctcgttgtac acgcggaata cgccgtcgtt gtgggtggtg cggtaggcga actcctgctc
     9301 cacctgcccg gaaagctcat agccgtaggc ccggcaggcc tcccgcgcca tgcggatgcc
     9361 tccgaaagga ttcacaccgc gcttcagcgg cctgtccgtc tgcaggccga cgatcagctc
     9421 gttgtctttg tccagatagc ccggcttgta attgagcagg gaggacaccg tctgggtgtc
     9481 cacatccagc acgccgccga actcctgctc cagcttcatc aggccctgca gcttatgcat
     9541 cagtgcctgc gtgcgcggtg ttgccggcgc caggaacgct tcatctcctc cgtagggtgt
     9601 atagtttttc tggataaaat cccgcacatc gatgcggctc tgccacgccc cggtcgtaaa
     9661 cccttcccat gccttgcaca tttcctgtac ctcctgttcg ctcacacacc gcggaggcgc
     9721 ggcccctttt cattcctgta ccgtcccggc ggcggccatt gagaaaatct ttttgtttca
     9781 gggcaacaaa aaagggcagc accgcaaaag cggtgttgcc cagacggccg gcatcaaaac
     9841 tccgcgcccg ttcccctgtg gtaagcccac tcgttccgtc agttacggcc gcgtctcggt
     9901 tatgtattca tgataccacg cttcggcaaa ggctgtcaac ggacaaacag ccgaatccgc
     9961 aggccgcaca acgcaaaaat atggttaaac cggccttcga gatgccaacg ctctgcttta
    10021 cgaaggatgc ctgtatgtgg ataacataaa attggtaaat tggaaattat aacagtcctt
    10081 ctgcttttgc acgaattgca aaaaactcct gaaacgtatc tgatttttcc tgtattttct
    10141 ttttcatcaa ggtcaatctc cgtttgccaa cccgcctatc caatacagca aatattctta
    10201 cgattaaatt agcactgtta aggctcattt caatatctta atcatcaaac tctgcaaagg
    10261 cgttgtaaaa acagcgttga ccaaaaacac ttagtcttaa agctgtatca tccatatgcg
    10321 cattttccac ttccatgcgt tttttgtatt tttcatctcc gggaaacaaa tctgctttca
    10381 ttcagttatt ccaataacat cccttgatga tttcgttgcc atcatatctt attgccgccc
    10441 gcccctcatg gtcaggactt ctgctatacg acgttgcgta atacggaata tggccttgca
    10501 gactctgcgc caaatattct gtttccagct ttttcctgat accgctccct gttgccattt
    10561 gcggttcttc ctcttcagct tttgattgtt ttattgcccc atttcttgcc ctgtcatgca
    10621 gaaagcatct cctgttccgc ctggccgatt ccagtttgcc gggccgccgg gccgtatgaa
    10681 agcggctgtc tgccttcgtt ttacgcctgt gtcgccgtgt cccgcaaggt tcatccgccg
    10741 cttatccccg gcagaacgcc ctgttacagc gtgacgccca tcaggtactc ctttacctcc
    10801 agcagctccc gcgcgggcag ggaaaaggcg cgcacgccca ggcgggcata ggcctcggcc
    10861 agcgcgggct gcgccgcgcc ctcgccgcac aggcacagcg ggatgcgtgc ttcggccgcg
    10921 gcctccacgg cgaaccgtac aagccggtat acggcggggg aggcagcggg cagataggcc
    10981 cgcacctggg ggttcacacg gtcggccgca tatgtatact ggatgagatt gttggtgccc
    11041 acattgaaaa acgcggcgcg gcgcgccagg tccccggctg tcagcgccgc ggcgggcgtt
    11101 tcgataaacg cacccactgg aaccgtttcg gcaaagggca cgccccgctc ccgaagcgcg
    11161 catttggcac ggtaaacagc gtccagagca tgctcgaact catcccgcgt agagaccatg
    11221 ggcagcagga tgcgcagatt tcccgccagc ccggcgcgca gcagcgcccg aagctgcgtt
    11281 tcaaaaaaat ccggatgcgc caggcagtag cgcacgccgc acagccccat cgcgggattt
    11341 gcttcttcct cccgtggaaa ttccgtcccc gtcttatccg gcgccacatc aaaggtacag
    11401 atggtaacgg gcctgccctg cgccgccgcc agacaggcag catagaagcc atactgctcc
    11461 tcctcgccgt tcacgcgccc cgccagcagt aggtattcgc tcagcagcag acccacgcca
    11521 tccgctcccg cctccacggc cgtgcgcaca tcctccaccg acgcgcagtc cgccatcagc
    11581 gagatgcgcg tgccgtcctt cgtggtacac ggcactgccc gcagccgctc ctgcgagagc
    11641 gtgtgccgcc gctgcaagcg cagtttgtgg gcaaagcgtg ttttcgtcgc ctcatccggg
    11701 tccagatacg cttcgccggt gtctccgttc agcgccagcg tgcggccgtc acagccctcc
    11761 agcagctccg gccccgccat caccgcggcg gggatgccca tggtgcgggc gatgatggcc
    11821 gcgtgggcat tgggggaacc cgcgctggtg ataaacccca gcaccaggct gcggtccagc
    11881 gtgacgatat ccgtgggata cagctcctca gccaccaaaa tggccgggcc cgtcaggcgc
    11941 agcgtctcgt ggggctggcc gtcgagtacc ttgacgaccc ggcggcatgc gtccaggata
    12001 tcgcaggcac gctcgcgcat gtacgggtcc tccagtgcac gaatgcggcc cgcaaaaatg
    12061 cccgccgcgc gctccaccga tgccgccgca cccgcgccca cccggatata ggacgccacc
    12121 tcctgccgaa ggccctcgtc ctccagcagc accctctgca ccataaaaat atccctgtcc
    12181 tgtgcggcag cgcgccgctc cagccggatg agctggtcct gcgcctcccg caccgcgttt
    12241 tcaaacgcct cctgttcctc cttgggggac cgcaccgcgc gccccagccc gctttgcgta
    12301 tggcgcaaat accgcaccgt ccccaccgcc agccccgccg aggccggcac tgccgtgata
    12361 tacttcaaat ccgcgcacct cccggtttcg cggcagaggc cacaagccgc cccgccgccg
    12421 tcctcttcct gctccaatgg ttcgcgtgcc ttgaaaatct gtttttgttc tttttattcg
    12481 ggcattgcca taccaaaacg ccccacatcc gcaaagccca gcgcgtgata gatgcgcccc
    12541 gccgcggggt tgtcgtagaa caggcacagg taccgccgcc cggcggcaaa actgtgggcc
    12601 gcggccgcgt gcacacaggc acgggcaaag cctctcccgc gccatcccgg cagcgtcgcc
    12661 acaccgacga ccatggccga aacgctgttt tccgcagtcg tctctgccag cgccaccagc
    12721 ctgtccccat cgtacacacc gaaggcatgg ccgccgcgcg tcaggttttc ccggcattcc
    12781 tccacctttt cctcccggtc cacaccgcgg aagatgtcgg caaattcatc gatctgtact
    12841 tccagcccga tcacttcctg cgcatcctgc ggcccgagca tccgcagaga aaagccctcc
    12901 ggcagtgccg cgccgccggc caggcggtcc atgcgcgcca gattcgttcc gcgcagcttc
    12961 cagccgggca tgcgtgcggc caaagcggcc acatctgctt ccttgcccga aagatactgc
    13021 gccccgcggg cttgcagaaa cgccgccagc tcctccaccg gcgctgtccc gctcatgcac
    13081 agacagtaat tgttgaaata ccgcagcagc agcgcacaaa gcgcgccgtc cgcagcacgt
    13141 gtgccgaaga tctccaccgt tttcgattca aagccgtaat tttcaaggtc gccttgcaca
    13201 aatacattgt attccggttc ggcagagata tatgcctcta cctcggcccg gttctgctcc
    13261 gtcagttttt ccattccttt ccactccttc tctacattgt cttccgggcg cggcggtcgc
    13321 cctctcccgc ggcatgccgc ccctttttgc tccgcaataa aagtcccctc aacggccatt
    13381 cgggccctcc cggcccacgg ccttaaggtc tcaagcgccc gggcacaaag cgcccggatg
    13441 cgtgtaaaaa acagcagctg tgctgctgtt ttttattagt atagcacatt ttgcacatac
    13501 ccggaaatcc gcattgagcg ggattgcggc gttgtttttt cgtttttttg ttcaattggt
    13561 aaaaaatgcg tttttctgta aaaaaaatcc atcaaaaata ttgctatttt cacttcatct
    13621 tgctaaaata ttaaaataat ttttccggcc tgccggaaga attatggctt ttacaaaacg
    13681 aaaagaaaag gtggtgtaag tcatggagat caaaggcgat caaatctggc acagtccgtc
    13741 ccccgcagag ctgctggccg ccgccgggca ggaaatcacc ctcaaggcgt gcgttcacaa
    13801 catccgggag cttggcggca tcgcattcgt ggtgctgcgc accgggcgtt atctcattca
    13861 gtccgtgtac gacaaggagg tgtgtccgga taataatctg agccagcttt ccgcaggcga
    13921 ctgtgtccgc gcaacgggcc tgctgcgcgc cgaaccccgc gctgaaaacg gcgtggagct
    13981 gctgctccac ggcttcaccg ttctgtcccg tccggcttcg ccgctgccgc tgaacctggc
    14041 cgataaagag ctgaaggcca cgctgggcac caaaatggag taccgcaccg tggcgctgcg
    14101 tcatcccagg gaacgcgcgg tattccgcat ccagcaggct ctcgcctgcg gcttccggga
    14161 gttcatgctt tcccaggatt ttactgagat ccatactccg aagatcacgg gcatcggtgc
    14221 cgagggcggc gcagaagtgt tcgagctgga ctacttcggc atgcccgcca cgctggcgca
    14281 gagcccgcag atgtacaaac agacctgtgt cgcttttttc gaccgcgtgt ttgaagtggg
    14341 agccgtctat cgggcggagc tgcacaacac cagccgccat ctgaacgaat acacaggcct
    14401 ggactttgag atgggataca tcgacggcat ggaggacgtg atgcagatgg agaccgccat
    14461 gctgcagcac accatggcct atgtgcaaga acactgcgcg ccggagatcg cgctgctgga
    14521 cgttgatgtg ccccggatcg gcgcaattcc ctgtatccgg ttcgcagacg cactggcttt
    14581 gttgaaaacg ctcggcggcg gcaaaaaccg gaacgacctc acgcccgagg acgaagtgct
    14641 gctgtgcgag tattacaggg aaaaggggct gggcgaattc gtgttcgtga cgcattttcc
    14701 cacggccaaa cgtccgttct atgtgatgga cgacccgcac aaccccaggg aaacgctcag
    14761 cttcgacctg ctgttccgcg ggctggaggt gacgacaggc ggccagcgca tccacgatta
    14821 tgacgcgcag gtggccaaga tgcaggcccg cggattggac cctgctcaat ttgaaagcta
    14881 tatcggcgta caccgctgcg gcctgccgcc ccatggcggc ctgggcatcg ggctggagcg
    14941 cctgaccatg aagctgtgcg ggctgtccaa tattcgcgac gccagtctgt tcccccggga
    15001 catcaatcag ttcatggcta attaaaggga acaaaaagaa aggaaaagca caatccagac
    15061 g
//
LOCUS       DAWBSB010000024        14196 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1063_length_14196_cov_2.909342, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000024 DAWBSB010000000
VERSION     DAWBSB010000024.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 14196)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 14196)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14196
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1063_length_14196_cov_2.909342"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    782..791
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1268..1277
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5656..5665
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7837..7846
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13165..13174
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2696 a         4217 c         4512 g         2721 t
ORIGIN      
        1 gaattccaag ggctgttttg gtgcgccaga ctggactcga accagcggcc ttccgggtcg
       61 gagaactgtg acagacagat ttaaatggcg taaccgtgct gcttgtagat gaaatgcaca
      121 agttataaag actggtaaaa agcccgcgaa gccttttgcc gtcggccttc gcaggctttg
      181 ttccagtagc agtcaaacag tagtcagaac cgttgaatca ggccttgttt gaaaaatcag
      241 gaaagctata cttaatcaac aaaatagcga tagaggccag gtgaacaagg atcgctactt
      301 tttttcgttc ggagcgcgcc gcttaaggtg caaaagcagt atgagcattc ccgcgcacaa
      361 tattgcggca aggctcaaca gaaacagagg cgcgtagtct cctgtctgag gaacgttgac
      421 ggcggatgga ccgtttccgc cggaggatga cgtgccgctg tccggagcgg cgcccggagc
      481 ggaagaacag ccggaggaag gagcgcccga ggaggatatg cccgaagaag cactgcttga
      541 agccgggccg ccggagctgt cgtcagagct cacatccggc tccagagagg tgcggtacgg
      601 ctggggggaa taggtgcccc gaatggcctg ataggtaaag tcatacagcg cacgggccac
      661 ggggtcggct ttgccggtat aagggtttac tcccgtggac gcttccagca gcgttttgat
      721 gccgttgtac cagtttcggt atacgcggct gtcgggcccg atgaaaccat atttcacgcc
      781 tnnnnnnnnn natccagata tttgttgtat ttatccagat cgttgaaaac gcggtcgtcc
      841 atttccagct ccatacctag accgtacatt ttggcaagtg ctgccgcgtc gcggatacgg
      901 tcctgcgagg ggccgcccga ggggaagaaa tggttgggct gcagagcgac ggcggagagc
      961 cccatatccc cgccgcggtt ccagccgctg gcattgtaaa agggaatcca aagcatggga
     1021 tatcccagcg tctgcgcctt ttcggatgca taacggatga gtgaactgcg gtagtttgtt
     1081 tcatgcatcc agtaaaagcc cgcaaattcc aggtttttat aatccgccgc ttcaaaacgt
     1141 cgcagtgctt ctgcaagata ccagttcagt gctttctgcg cgtctgcctc cccggagaga
     1201 ttcagcgccg cgccgtcaag catgccgaaa tctgtgcaca gcgcgtcggg gaaggggacc
     1261 ataacggnnn nnnnnnntca ctttcatttt aagccccgga cgccccagct cggcggctgt
     1321 ttctgccaca gcggcatcca gcgcgtccat gtcgccgccg cggtcgatgg tcttggaaat
     1381 gtagttctgc cagtcggcca ggttgctggg cgttgcgccg tagtcgctgc tctcaatgaa
     1441 cagatgccct gtctcaggcg aggactgtgc caggaacaat gcgctgtcaa acatcacatc
     1501 ctgggcgcgc ccgctttcat ccacataggc tgcatagggc ttgcagtccg ccttgctcca
     1561 gttgccgtaa ccggggttgc caccgtaatc cttgtagggc ccgttgtaca tcagcaccat
     1621 gtcccggatg ccgcctgtct gcggcccgga caaaaggaac gcgccttcaa agtccgcgtc
     1681 ctgaggcagg gtcaccgaat ctccggcttg cgtctggccc aagacctcca gttcgtcgaa
     1741 aaacagccag ccgttcagct ccgcgtccac gcgcacatac cgcgcggcca cgcgggcggc
     1801 gtctgttttg ctgccgggct ggcccgcgcc gcccagccat tgcagcgtgt gcacgccgtc
     1861 tgccgctgcg gaggtttttt catcatacag cgcggcccag gtgagtccgt cggaggaaac
     1921 atacacagaa aagcgcgagg gcaggcagat gccggagccg cttttttgca ggaaattcat
     1981 gctcacctgc tccaccgcac agatttggcc caggtccacg actgcggtgc gcagcggcca
     2041 ttcgcccgaa tgtccctgcc aggcggcgtc atacatgtcc gtgctgccga aggatgcgtc
     2101 cgtcagctcc ttgccgtctg tgtcgccgta ggcgtccggc gcggcgtggg agactgtata
     2161 gggcttgtcc agcgcaagat tctgcgggcc gtccggtgtg ccgaccagcg tgccggctgt
     2221 tgtgcatttt ccgtttttgc ccatgacagt cagctcgtcg aacgccgtcc atacgcccga
     2281 acagtcgaac gagatgcgca gatattgaaa atagaccata tcggcgtttt ccgttgtgga
     2341 gatgaacgcg tccgttgagc cgttccacac atattcgccc acaccggggt cggttacgga
     2401 aaacggggca ggcccgaaac tcttgagcgt cacccatttt gctttattgt ccgagacctc
     2461 cagtttcagg ccgcggggca gaaaaacgcc gttggcggta tcataaagag cgcgcagacg
     2521 gatttcagac acggattttt tgccgccgag gtcatatacc atcgctgtgc ggttggcgtc
     2581 cggtgcggcg ggatttgtgc cgttgaagct cagccagtcg gagtcgttcc aggcagggcc
     2641 tgccgtcaca ctgtccgtca ggatcgtggc gggcgcgccg tacagcagct cgcgcggaca
     2701 ttccaaagca aggccgtcgg ccagatccac gctgggcagg tcgtccggcg gtacgtcggt
     2761 gccctggtcg ggcgtttcac ggtcgaatat ctcaatctcg tcgatgaaca gccagatctg
     2821 gaaattgatg tgcagtttta cataccgggc ctgcgcctcg gggcattttt cctggggcac
     2881 ggtgtagacg aagcgcttga tgccgctgcc ctcgatgatt ggggcgacat ccgtttgggt
     2941 cagtgtgcgc cagcttgcgc tgtcgtcgga aagctcgatg cgcacggaat acggcgtcgg
     3001 gatgccggag ccttcctcgc gaagaaaatt catcttgacc tgttcaaagc gtttggattc
     3061 tcccagatct acgatgaatt caaagccgtc ctgcacctca ttgtaataac cgacccattc
     3121 cggcgctttg caggaaagcg cacggctgta aacaccgtcc gtgagctgcc cgccgtcatc
     3181 cgcgtagctg ccggaggctg cccacgcgct ggtatacggg cgtccggcgg caaggtttac
     3241 gcagtcgccc gggtccgggt ccaccacggg cggaggctcg ggcgtatcgg cttcggtgcg
     3301 cacgtcgatc tcatccacgc agacgtaatg gacggcgccg tcggtgatct gggacgtcgc
     3361 gatatcaaat tcatagcgca gataccgcgc tgtgacaggt gcgccctccg ggcaggccca
     3421 ttcgtggcgg tggccccact cggcagccag ctccgcattg tcgaaggcgg caagcggggt
     3481 ataatccgta ccgttttcag aggcggagag cgtgaggttt tccatctttt ccgcccagcc
     3541 gttgacgatg gtaccgatgc cggaggcgta cacagtgcgg gtttcgtgca ggtctacggt
     3601 gaatacgaca tggttgtccg tgccgtcggc agccgtctgc acgtagaccc agccctcgcc
     3661 gtccacattt tcctgcgcgc cgtatacacc atccgcgagg ctggcttcgc tgccgtaata
     3721 aatggcgtgg ctggaattga cggtcatttc agggtcgagg gcagggatgc ttttggccag
     3781 ggcgggcgtg cccatgcacg caacggcaag ggccagagcg gcgcacagca tgcgccgcag
     3841 caagggatga tgctgtttca tgattcctct ccttttttat gtaatggcag ctttttcacc
     3901 aagctgcctg cgaacagggc cttcggaccc gtttgccgcg cacggcgggc ttcggcggtc
     3961 gcttcgctct acacatctcg gcactggccc cgcggggtca ctttaaatga aaaaagggtg
     4021 tctgcgcgct aagagccgcg ctttcagcgc ggttatcccc aagggaggct tcgcaccgct
     4081 ccgatacgtg tctgcgggca cggtcgccac cccttttttg gaggcattcg ccgcgtggcc
     4141 tgcactttgc tgcgcaaaga tgcatggctt tgccgccgcc agtttttttc cggacttttc
     4201 ttttacttct ttgccttttt tgacagtatg ctataatgac gttctttggc gatttggttc
     4261 gtgccgcgct tgaatttttg tggtgcttga aaacagagaa gaccctctcc ggcgaaggat
     4321 acaggcttat ccgcgcaggc gcttccatcc acgtgccgcg gccgcacaca aaagagaggc
     4381 tgccagcgcc cctgcgcaga ggcccagcag cgccgcaggc cagccggagc gctccagcag
     4441 caggcccatg ccgtagcctg aaagcgtgct gcctgcgtag gtggcagagt tgagcagccc
     4501 gctcagcgca ctgatgcgcc ccgtgttttg cagccgcagg ggcaccagag tgacaagcat
     4561 ggtgtttgcg gccagcatca gcgcggcgca caggcccagc agcgccagac aggcccagag
     4621 cggcagccgc acgcccacgg caggagccgc cagaagggcg catgcggcca gaaacgccgc
     4681 tgcggccgtg acggcttcgc tgtgaaatat atggtcgttt gcccatttgc ccatgtatac
     4741 gccgcacagg ttcacaaggg gaagcgtgaa agtgagcacg atggcgaagg tgttgtccag
     4801 cccgtaaatg tcatggaggt atgttggcgt ccaggtctgg atgccgtcgc ggatcattcc
     4861 gcttgccaga gccgcaaggc agatgagcgc cagcacggcc ggaagacgat ggggcagggg
     4921 gcgggctgcc gcgcgcgcgg cgctgcgggc gggtgcggcg ggcagcggag gcgccagaac
     4981 acgcatcccg cgcagccagc acagcgcggt ggctgtgagg aacagccctg ccgcggcgaa
     5041 caccgcgcgc cagcagccca ttgcggaaca gagtgccgcc agcccataag ccgccagcgt
     5101 gccgaagggc accgtcgtgg agtagcgcac gctggcgcgc atggcctcta caccgggcag
     5161 gcgttcgaca gtgagacgca gaagcggaga ccacacagcg gactgtgccg cgccgttcag
     5221 tgtccaaatt acagccataa ggccggggga agaagcgcag gccatggcga ggttcagcag
     5281 gccggagcac agcacaccgg aaaacaccat gcgcacgggg tccagcctgt cgccggcaaa
     5341 tccccagacg agctggaaaa taccgtatcc gacaaaaaaa ccgctgctca cgaggcccag
     5401 ctcgctttgt gaaaagctcg ctgccgcaga gattgcagcc aggctggccg tgaagttgag
     5461 acggccgata taagtgctga aataggctgt ccagcacagc gcgaaaaaca ggtgctgccg
     5521 gcgcagcccg gtgatttttg cgggataagt ggcgttgttc aaggatctgc ctccgtcttt
     5581 tcattgttcg gcttccggcg cgggggccgc cccggtttat aagcgcgcgc cggacgctcc
     5641 gctgcgcaga tccacnnnnn nnnnncgtcc ggggtgcagg aagcgttgta gcattcaaaa
     5701 tggccgtccc cggtctctgc gatatatgct ttggtgagga caagctcgcc catggccttg
     5761 aatacctcat gcacatcgtt gtcgcaggcg gcgccctgcg tcaccgcgcc gcccatggcg
     5821 gtgtagcctg agacctgcgt gtgcggcggc gcggtccacc agctgctgat catcggcacg
     5881 tattcctcgc cgatggtgcg ctccgcatgt ctgcccgcca cccggccctg cgccgcggcg
     5941 gcttcatcct ttacaaaaac acgtgtctgc cgctgggggt cgggcacgaa cgcccagcgc
     6001 agtgtgccgt caagccccat cagctgtgcg caggcgccca tggcgttcat gccgcgctcc
     6061 agatatcgca cttcgcccgt gagctcatac aggtggaaca ggccgtagat attccaggcc
     6121 gaccagccgt ggggtgaatt catcatttgg gcacggggcg ccgaaggcgt gccgcccatc
     6181 tccacatcgt attgcgcctc ccaaaagcgc agcgtgccgc ccgccatccg gcaatccggg
     6241 acaagcgcct gctcgtggga ggcgtgcttt tccagaatcc ggcgggcggc ctcggtatag
     6301 cgctccctgt cggggctgtc ttccgcaagc atcagcgcga attcgctcag ctgtgcggcc
     6361 gagcatgaat tcgccccgtc ttcgtagcag gacgcggtag cgccctccgt ttgaaaattc
     6421 ccgtccagcc cgacaaggtg ctccatggcg cggtgcagcg ccgccatatg ccgctcggcc
     6481 cgcccgcgcc acagcgcccg gtcttcgggc ggaatgcggg cgtcttcggc gcgggccagc
     6541 tgtgcccgga caagctccat gatggatttt gcagggtaga tgacggaggt ataatccgta
     6601 tcccccttat agaacccgcc gtcctcccgc tgaaaacgaa gcacgagctc cgccaggcgg
     6661 tgcgcctgct ccaggtctgc aatgtccgct gtgcatgcat accggcagac aaaaataccc
     6721 agcatggacg agtggttctg aattctgtcc tcctgtacaa gaggaagccc ggtcccgctt
     6781 tggtacagaa gcgggtatac ctcatgaaag cgccgttcga gcctgccgtc gagcacggcg
     6841 tcggggaaat acctccgcgc ggtgtaggca gaataaaagc cgtaccagct ctccgcgtgc
     6901 gtggaggcct tctgcggcgc gtcggcagcg gccttgcggg ccgcttggat gtaccatgac
     6961 caggggcggc gcacaaagag gcacatttcg gcctcatacc ccgccgcatt gcgcacacgg
     7021 gccgtataaa ggccgggggc ggctcctgcg gcgcggaaca gcgcgcggta gacgcccggc
     7081 ttgtccggct caagcggcaa acgccgcgtc ccgccgtcgg ggccgttcag tgtcagcgtg
     7141 ttttccggca ggggcgaacg ggaaaagacg gtcacgcggc acgcctcgtc cgcggcgagc
     7201 gtatagcggc cggcgtgcag caccggcaga agcgacgctg cggcaatggc gggtttgatg
     7261 gctccaatgc cataaagcgg ccccacgggc agaagcgtga cgtgccaggt ctttttttcg
     7321 ccggcggaaa gcctgtcaag gccctgggga tgccgggcgg gcagccgtcc cggctggagg
     7381 agatccagcg aggccgtgaa gatcccctgt gcgttttttt cataatccag cgtgtaggac
     7441 gccgtgggcg cgtcggtgaa cagggccagc agtctgcccg agggcgaaga aaaataaccc
     7501 cacaggtgcg ttttttcaca gcggagcagt gtggggaaaa gctgatcgtt gtaatcggga
     7561 tactgtgcca gaaatgtatc gaagcccagc cgcaggctcg cccggaaggg cggcgtggcg
     7621 gtgtccgcct cgttctgcaa ggctgcggaa accgtgagcc gtcccgcttt gtccgccgcg
     7681 taggaaagcg acagcgcaag cgcgagcccg tttgccgaaa agcggccggt gtaaacgggc
     7741 cgcccgctgc gccgcacggc cgacagcggc acctcggctc cgtctatcag ccagcacatg
     7801 cccgcatagg cgtcccggcg catagggacc ccgcagnnnn nnnnnncccg cagagcgcgc
     7861 cgccaaagcg ggcgtgcagg cgtctgagcc tgccgccgcg gtggtcgacc agccagtccg
     7921 gggtgcgaag aatctcattt ggaacatttt ccatagccgt cctccgtttg gatgcccgcc
     7981 gggcggttca aagctccacc gtcatgccgt cgtaggcggg ctcgatttga tagggggccg
     8041 cgttccgggc gaattcatcg tgcagccagc cgccgttgtg ggaaaaatgg ttcgcgatga
     8101 aacgtgtatt cgggcccgcc gcgccggctt caagaagccg aagccgcacg gcggccgcgt
     8161 cgggcaggcc catatggccg tcgttctctc cgttttcaaa acggccgctt gtggagtcca
     8221 ggctgacggc gctcagcggt gcggcgcgcc ctgccagaaa ttcatacacc tccggataga
     8281 accggccggt atcgtgggcg tacagaaggc tgcggccctc ccgctcgatt aagtaaaaca
     8341 gcgcctgctc ggtgtccatg tgctttgcgg gaagcggcgt tacacgatat ggcccggcag
     8401 aaacgcattc aaagggccgc acgatatgaa accgcaggcg ctgctccacg tcgggcacaa
     8461 gctcatgccc ggcggccttg tagaagtaat cccgcacggc ctcgttgcag aagatatcca
     8521 gcgtagggct tttcatgccg tgggaatagc atccgccgcg gttgacaagc tcggcggggt
     8581 aaaagtggtc ggtgtgagaa tgggtgacga gcagccagcg cacggcggag aagtccagcc
     8641 cctgccgcag cgcgtgcagc caggcgtcgc aggggaagtc cagcagcagc gcgtcgtcca
     8701 ccagcgcctg ggagcgcgtg cggatgttcc tgccgcccag ccgccgcgcc gcgcggcagc
     8761 cgtcacagtt gcagaaagcc gcaggccagc cctcggctgc ggctgtgccc agataggtaa
     8821 gcttcatggc atatcctcct tgttccggcg ctgctgagga cacgtccggt aaaattttac
     8881 gctcaccggg gacaggcgca gcggttccgc atctgtgccg tccaatgcat tttcaaggcg
     8941 caggccgtcc ggcagcacgg cgcgggcatc gtccaggccg cagtttacaa ggcccagcag
     9001 gccgccgccc tgtgcgtcct cgtaataaaa gggggcgagg ttcgcgtccc cttccacaaa
     9061 gggcagctcc gcgcagcccg gcatcccgcg cagcaggccg cgcagcagcg cggcgcggcc
     9121 gcggcacagc cagcccagcg tgcgcaccgg cgcggccagc acgcagacaa tgccgcccaa
     9181 tgcgttttca aagcggatga gggcgggggc gaggtattgc ttttcctcat ccagcaggcg
     9241 gcagatctcc tgcgcgccgg ccgccgccgc gaagcgtgtg atccacgcgc cctcccggcg
     9301 cacggcctcc caatcggtgc acagcagcgt gcccgcccac tggcgggcat attctgcgga
     9361 ggagatctgt tccacgcagg gcccctcgat gcggccgtcc ggcgcgcagc ccagcagcgg
     9421 tgcgaagccc atggcgcaca ggtgttcggc ggcgatgttg tccaccatca gtcctccgcg
     9481 caggaaggct tccagctccg ctttcttata gcacagcacg tcgtcgccca gcagaacgtt
     9541 ggtggcggcg ggcgtgaact gtacgggaac gcccagcagc ggaagcaccg cgtccagcgg
     9601 ccgggcgggc agcatgccgt cgggggtgtg cgcacggttt tggcggtgca gcgccgcgtc
     9661 cccgcgccag ggcaggccca cgccgcgcat cgtcttgccc ctgcgcagct gctgaacggc
     9721 ctccaccgca ggcgctgctt tgcacaccat ctccgcgtat tcgcgctgca gcggcagcgg
     9781 tgtggcaagg taatcgtgca ggttcagcgt caggccgtcc gcgcccgcaa gcgcgtggag
     9841 ctgcatctgc agctgcgtga cggcagcgct ttttgtgtaa ggcgtattgg ggtagttttc
     9901 cacctcgctg gcccagaagg tactgctgtg tacagcggcc atgctcagag acatgcccgt
     9961 aagcatttcc acggcgccgg tgtgcagcgc gtcgctgtat gcgccgccca gcgggcggga
    10021 aaggcattgc ccgcccccgg aaaaggcgcg gagcagcgca tccatgtcgc gcccctgcac
    10081 cgaatgcgcc ggctcgccgg agttcatcag ccccacctgc gtcttgggcg agacggcgtg
    10141 cacagcccgc tccaccgctt ttgcggcccg cagctgggcg gcaaagttca cccgcatcca
    10201 cgcggctttt acctctccgg catcggggcc gaggccgcag gccgcgtccc gcagacggag
    10261 gggcgtgacg tcgtacccaa gctcccggga aacaaggcgg gcgtgggttt cgcaaaagca
    10321 gccgtatgga gccgtgtggt tcagcgagcg gaaatcgtcg tccagcatca gccgcgcggg
    10381 ccgggtggat gcgtacaggg cgctgatctg cgccgtatat tccacccacg cctcatcggc
    10441 gatgcagtat actgcgtgcg cgggcgcaag gtcctccccc acaaagcgct ggaagggaag
    10501 cataaggcgg gcgggcacac ggttgtcgcc gtgcccagcg caggtgacga ggtttacact
    10561 gtgccgcacg cccgccgcgg caaatgcttc ggccatacag gcgtagacag cagccatgcg
    10621 ctcgtgcttt ttccgtggga acggggaggt gaggatttga tgcgattcct ccatcagcat
    10681 cacctcgcgg atgggcgtgc gggcgcaaag ctccagcagc tcaccgagct ggcgctgcca
    10741 ggcatcttcg tgcacggcaa cgcgcagaat atattccata agagtaccac cttttctgtt
    10801 tgatgcggga tttgtaaaaa aatagggaga ccgatgcgcg catcggcctc cacagcatgc
    10861 cgcgtcagcg tgcgaagcgg tctatttgga aagctccagg aatttgtcat actgagactg
    10921 ttcgatctcg atcagctcgt ccagccccag ctcctgcatc tgggcaatat actggtccca
    10981 atcgtcaaag ctggcgcgtc ccaggatgaa atcggtcaca agctcgctgg aataggtgga
    11041 aaggtctgtc gtcagagcag ccttgcgcgc gttctgctca tcggtggcaa tggccatgta
    11101 gcagtatttg gcatccggat aaaccgcgtc ctggaagacc acgctctttt gaaattcgtt
    11161 tttgtgctcg gggcgggagg tgaactggga ttccattgtg tacagggaaa cggtggggaa
    11221 tacgcagccg ctccacagcg agccgccggg ggttttgcgg tccttcgcgt tctgctccca
    11281 gtacgcgccg ccgatgccct ccatgagctg gcgccggccg tccacgactt cgtacgtttc
    11341 gccctcgatg ccccaggcgg acagctcttc ataccggtcg gaatacagca cgtccagcag
    11401 cgcggctacg gcctcggggt ccttgcagtc tttcgtaacg gcccatttct gccaggagaa
    11461 gaacgacggc tcaatgacaa tgtacggctc gtacccggtc tccaggttcg caatgggcaa
    11521 aaattcaacg ttttcagcgt tgacggaagg ctccagccac atctgcatgc agtaatcgaa
    11581 cgtggcgccc actttgtttt cggccatgcg ctggctcgtc tgttcgtagg tggcgccgat
    11641 gaggtccggg tccatcaggc cctcctccac aaggcggttc atgtacttca agtagtcttt
    11701 gataccgtcc tggtaccagg gcgagacgat cttgttgttt tttacgtcca cagaagtgag
    11761 gtaattgcct acgccgaacc actgggcgat gcccgtcatg aagaaatctc cggaggtatc
    11821 cacggtgacg atctcgtccg ctgcgccgtt gccgttcatg tcattttcct gaaacgcttt
    11881 catcacagag atgaactcct cggcggtttc aggcgcttcc agaccgagct tctccaacca
    11941 gtccttgcgg atgttgatga cgcggcaggt gggggcgggc ttgtcttcgt aggtctgtgc
    12001 ctgaacgctg gtgtaccagt agatattgcc gtccggcgcg gtggtgagct gtgccatgaa
    12061 cggatatttt tcgtgaatga agcggttgaa ggtgccgtcg ccgtactcgt ccatgatggt
    12121 gttgatgggc tggatggtgc ccttgttggc aaggtccatc gcggcggcgt cgtcaaacgg
    12181 cgtcagacat acgatgtccg gcagatcggt gccggaggcg atgcgcgttt gaagtgttgt
    12241 ggggtactgg tccgctggga cgacctcaaa ttccagctcc aggccctttt gtgcaaacag
    12301 ggctttcagc tcctgccaga cagggtagtc ctcgcgctcg tcgaatttga tgtactggtt
    12361 cgtgttgttg ggcacaagca tcgtcagacg ccgggcgccg tccccggcgg atgcagggcc
    12421 ggaagccggg gcggacgatg cgccgccgca ggcggccatg gaaagcgcaa gcgccgccgc
    12481 aaggaatagt gcggtaagtt ttttcagatg tttcatgtcg ttcctcctgt ttgatgacat
    12541 tttatcatac ttcgacccga ggcgtcgggg cgaaatctgc cttgaagaga gtcagccctt
    12601 caatgagccg accatgatgc cctttacaaa atgcttttgg aagaaggggt aggcaaacag
    12661 tacgggcaat gtggtgaaaa taatcatcgc atatttgagc tgcacgttgg aaagtgaacg
    12721 gcgcacggcc tcggccactt cgttcatggg cagctttgtc agatccacgg tgttttccag
    12781 cagcacccgg cgcaggtaga gctgcagggg ctgcagcgcg gtgtcgggca gatagaccag
    12841 cgcgttgaac cagctgttcc aggtgatgac gatggtgtag atggaaatga cggccaggat
    12901 gggctttgcc agcggcgtat agatgcgcag cagaatggtg aagtgactgg cgccgtcgat
    12961 ggctgcggat tcccggatgg tgtccgggat agaggcgaaa tacgtgcgtg caagaatgat
    13021 gttccagatg ctcagagacc caggcaggat gacgccccag atgttgttgt acaggccaag
    13081 gcggttcatt acaaggaacg tggggatcag cccgcccgaa aaatacatgg gaacgagcag
    13141 gtaggtgaca acggcctttt tgccnnnnnn nnnnccaaaa gcccgggcat gcacagggga
    13201 tacgcgcaca atgtgcagaa caggagcatc agcagcgtgg tgacgacaac gtacaaaagc
    13261 gtattgccgt aggcgcgcca cagagtcgtg tcggaaagga tgacgcggta ggagttaagg
    13321 tcaaaccctt tggggaacag cagcatgtcg cccgcaagcg cgtgctcggg cgcgctgaca
    13381 gagcggatga gcacatagta catggggtac acggtgacga tgcagcacag cccggtggcg
    13441 aggtaaatga gcatgctcgc caggcgggag ccttcgcgga tgccgtgttt ttgtcggagc
    13501 atggcggtaa gcctcctttt cgttttcaga acagtccatc gccggagatt ttattgctgc
    13561 atttgttggc gatgaaaaca aggatcagat tcaccgtgga ggcgaacagg ccgatggcgg
    13621 tgccaaggct ggtcttgccc gtgagcagac cctcgcggta aacgtaggtg ccgaacacgt
    13681 cggcggtctt catggtggcg ttgttgtaca aaagcaggat caggtcggtg ttggagctca
    13741 tgatgtcgcc caccgcgaag atgagcatga tggcgattgt gggggcgatc atcggcagcg
    13801 tgatgtgcag tgcctgctgc cagcggttcg cgccgtccag ccgcgccgat tcgtaaagcg
    13861 ccgggtcgat ggaggccatg gcggagagat agaggatgct gttccacccg aagctcttcc
    13921 agatgttcgt gaccgtgtat acaacaggga accatttcgg gtcggtgcgg aaggagacgg
    13981 ggtcgccgcc cagcagcgtg atgatctggt tcacaaggcc gtccttttca ataaaggaga
    14041 ccaccatgcc cgcggccact accatggaga tgaaataggg catatagctg gctgtctgca
    14101 ccagcttttt gtagcgtagg tgcgttacct cgttgagcag cagcgcgaac gctatgggga
    14161 cccagaagcc gaacgcgagg ttcatcaggc tgagcg
//
LOCUS       DAWBSB010000025        13934 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1077_length_13934_cov_3.061027, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000025 DAWBSB010000000
VERSION     DAWBSB010000025.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 13934)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13934)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13934
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1077_length_13934_cov_3.061027"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2596..2605
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5032..5041
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11308..11317
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2927 a         3875 c         4317 g         2785 t
ORIGIN      
        1 tgggcatggc ctttgtggcg ggcagcacgc gggtgcccag gcctgccgcg gggatgacgg
       61 ctttacggat cttctgcatg ggaaacattc tcctttgata gtttcttttt tacagcccta
      121 cagggcgggg aaaacatgag ccgagacttc ctcctccaca aaatgaacgg cggattcatt
      181 ccagtatttt tgatacaccc ggctgccgtt gccatccagg ttcagctggt aaaggcggaa
      241 agtgaccaag agatccttag gctgccattg ctcttcataa gaatactggt acttcatccc
      301 gatttgccgc atcacgccgc cgcttcgggg attgttgaca tcgtgcgtgg ccgtgatata
      361 gggcagcccg tcccgcttta cttgtgcggt gacggccctt ccggcctcgg tggcgattcc
      421 ctgatgccag aattccgcgc gcaggccata gccgaaatcg tggggctcgg ccatatctac
      481 cttgacatat ccgatgggtc ggttgccgcg cttcaggcag atggcgtagg cataggcctg
      541 tggctccgcg taaagggatg cgtagcggtc ttcaaagaat tttcgggcct catccatgct
      601 ttgcagggga aaccagggca gaaaccggtt cacatccgga tcgcggtata tttgatacag
      661 ggcctccagg tcattctccg tgaatttgcg caggatcagg cgttcggtct ccagtgtggg
      721 agtattgctc aatttgttcc tcccttttgc ccgaagggga ttttagtaaa gataaaaggc
      781 ggacatcagg cgatccggcc cgatccatgc ggaaaacgca aagctgcaca ccagcagcag
      841 cacgcccacc aggaccacgg ccacacggga cggcccgccg cggcgttcca gctcggtgcg
      901 gtagcggtcg tcgtcggtgc tgtcgtcctt catggcgcgc atccgcctgg ccgcgctctg
      961 gcggaacagg taaaaaccga acaggcacga ggcgatgcga acgccccagt tcagaagccc
     1021 gcacactgtg atgagtgtgg agagcgccga ggcggagaga ggcaaagccg tggcgatgcc
     1081 cagcgcagcc atgagcgaaa ggagggacgg gatgcttacg ataagggaca ccgccagcgc
     1141 cagcgcgccc cagccccaca ttttgcggta gaagaaataa aactccggga tgaacagcgc
     1201 gctccagcaa aagctggtct tgcgcccgct ttcatccatg cgctggaact gatacagata
     1261 atacggcgcg gaattgccga tgtacttcat ccagtcctgc gtggagatgc cgtcgtattc
     1321 cttggccaga tggagctgcg tcatcacgtc gccggtggcg tattgcgcgc ccggcccgcc
     1381 gaagccgccg ggcatgcccg tgccgctcat acccggctcg gcatactgtg ggctgctctg
     1441 cgccgcctgg ggcggtacgc ccccgcgcat gggctggccg caccgttcgc agaacagcgt
     1501 gtcgggcggg ttctgtgcat ggcacacagg gcagatcggc ccgttggcat ttacgtcggg
     1561 cttggcctcc ggcggcacgt actcaaagcc cttgccgtgc ttggcgctga acacgcaccg
     1621 gccctccgcg gcatagcatt cccggtgata gggcgcaccg cattccgggc agatgacgat
     1681 gtcgtctccc tctttcaata ctttatggca ggatgcgcat ttgcagcctt tgtaatccat
     1741 catgcagggg ttcctccaaa ttttcctata tatccctatc ttactccaaa caggccgtga
     1801 aagtaaaggg caaagcgcct ttacaagcgg ctttgtttca attataatag aaaaatatga
     1861 acaaatacca tactgcggga aaataaagtg aggattctat ggtaatttta gatgaagtaa
     1921 agcgccgcct ttccgagctg gcgccggaag taacggatct gcacgacgcg ctggccatcg
     1981 agcgcagcag ggtgcgtctg gaggagctgg agcagaagag cgccagcccg gagttttacg
     2041 acgacgcggc agcgtccggc gcggtgtttg ccgagatggg cgagctgaag gacaggctgg
     2101 agcagtacgc cgctttgcag accatgctgg aggatgccga aacgctgctg gaaatgtgtg
     2161 cggaagacga cgaccccgcc ctggcggagg aggccgacgc ggccgtgaac agcttggacg
     2221 ccaaggtgga ggagctgcgc cttgtgacgc tgctgaatgg agaatatgac gcgaacaatg
     2281 ccatcctgac cttccatgcg ggcgcgggcg gcacagaggc tcaggactgg gtggagatgc
     2341 tgtaccgcat gtacacgcgc tttgccgcac gccacaactg gaccgtgaag gtgctggact
     2401 atctggaggg cgacgaggcg ggcatcaagt ccgcgtccat tctggtggag gggcacaacg
     2461 cctacggttt tttgcgcagc gaaaacggcg tgccccgcct ggtgcgtgtt tcgcccttcg
     2521 atgcctccgg gcgccgccat accagctttg cctcgctgga ggtcatgccg gagctggacg
     2581 acgccatcac tgtggnnnnn nnnnncgggc ggacagcaca tcaacaagac aagcagcgcc
     2641 gtacgtctca tccatattcc tacgggcatc gtggtgagct gccagaacga gcgcagccag
     2701 ttccagaacc gcgacatgtg tatgaagatg ctggccgcga agctgtatca gatcaaagag
     2761 cgcgagcatc tggacaagat cagtgatatc aaaggcgtgc agaacgccat cgcttggggc
     2821 agccagatcc gcagctatgt attcatgccc tatacgctgg tcaaggatca ccgcacaggc
     2881 tttgaaaacg gcaacgtcaa cggcgtgatg gacggagatc tggacggctt catcaacgca
     2941 tatctgaaaa tgtccagcaa gggcgagctg taagcgcgcc gcggctgaaa aacagcagta
     3001 ttcatagatg tggatagaga aaagcgccct gttttccaag tgaggaaaac agggcgcttt
     3061 tgaaagctgt tttttgccgc cgccttatgc ggccgtgaat gggcaatggg aggaagcgtc
     3121 aggaagggag ctgtgcaccg cactgcgtac agaattttgc ctgcgcactg tacggcgcgc
     3181 cgcagaactg gcaataccgg attgccggcg gctgagggtc gggttgagcc gtggagggcg
     3241 gcactgcgtc aggtgcgaaa acaggccgcg gcgtgggggt gggcacgggc tgcgcaatgg
     3301 gctgggccgc aggctgctgg gcagctgccg ggtatggtgt ataagatgtg gcggccgccg
     3361 cggaaaatgc ggtttggtat gctttgcgga tggaagacag catggccgaa acctccggaa
     3421 cggagacgcg agacggatca agcgcgcgca cgacctcaca agcgatggcg tccagcggcg
     3481 gctccatttc gcgccggagg aacgcggcgt gatacaaatc atactggcgc agcgcattgg
     3541 tgtagatcgt ttcgctggaa ccaagaaacg caggaatacg ttccaatacc aacagatatg
     3601 cgttcattaa attggcgctt tctggcaaac cgcatacata agcagcggcg gggtaaagga
     3661 taaccgcctg aacgtgttga gcgggcatat aggcaaagct gcttgcggcg tgcgcctcgc
     3721 tggcagtgtc cagcaaaaag aggacttttt gttcgtatgc ggcaagaagc tgctggtctt
     3781 catcatcgtt ccagataaaa tagtacttca agcgggtgga tttatgaaag tagacgcacc
     3841 atgcggtgaa aaccaatacc caactgatga gctgtacaaa tatgagggga acgtccgcac
     3901 cgggaagaaa gaacattaga aaaaacaaaa aaatgttgac agcgccgatt gcatagtaaa
     3961 aatagcagaa ttcccgccgc cgtgttccca aaaatataag cataagaact tgcagcacaa
     4021 taaccagcaa ggtgaagaaa acccataggg atgcgcccga acggaggaac gagagggtcg
     4081 agttgaggtt gctgaacata cccaaaacct gcgtgacgat aaaaaatatc atccagccgc
     4141 ttaattttct gtattccaac ggtacggtat cgtaatattg cgtttgattc tgcacacgtt
     4201 ccaccccgtt ttttcggccg cagtactgcg cgggcaagaa tgcggcacaa tttatgaatt
     4261 aactgtataa taaagcacga attttgtcaa tccaacgggt gatcaaacac tgcgcctttc
     4321 tgcgtacagg cggaacaata aaatatagca gccgcgctgc tgttttctat gcacatccgg
     4381 gcactttgta cccagatgct tgaggtctta agaccgtggg ccgggacggt ccacctagcc
     4441 gcttcttata gctttgctgt gcaaagctat aaaaaaacac ggccgcctct gttcccaggg
     4501 cggccgtgtc cgtttttcag atatgacgga tctttttgtt gtacaggctg cggtagaata
     4561 ccactgccag cgttacggag gcgacctccg caatgaggaa gctgaaccac acggcgtcca
     4621 gcccgccgag cttggcaagc acaaaagcgc agggcaaaag cagcagcagc tggcgcaccg
     4681 cgctcatgac caggctgagt atgccgtcgc ccagtgcctg gaatacactg gaaaacacga
     4741 tggccacgcc cgcgaagcaa aagctgacgg agatgatgcg cagggcggga atgccgatgg
     4801 agagcatcgt gtccgatgcg ctgaacaggc tcagcagcgg gccgggcgcc acctggaaga
     4861 tcactgtgcc cagcgccatg atggcggtcg tcatcagcag cgagagccgc gtagcctgct
     4921 gaatgcgctg gctgctgcgc gcgccatagt tgtagcccac gatgggcacc aacgcgttgg
     4981 tgacgccgaa cacgggcatg aacacaaagc tctgcagctt gaaatacacg cnnnnnnnnn
     5041 ngcatgatga tggagggcag gcccacctga tagatgccct tgatggagtg ccagtccggc
     5101 cggaagcggc ggaagttgag acgcacctca tggtttttca catggttgat gccgatgcct
     5161 acggccatgc cgcagaactg gccgatgacc gtggcccagg ccgcgcctgc cacccccatg
     5221 gccggcaggc ccagccagcc gaaaataaag acagggtcca ggatgatgtt caccacggcg
     5281 cccgtcatct ggctgatcat ctggtatacc gtcttgcccg ttacctgcag cacacgctcc
     5341 atggtcacgg acatgaacag cccgagggag aacacggtaa cgatccacat gtagtccgtg
     5401 ccgcccgcga ggatctcggc gttctgtgtg aacgctccca taaacaggcc gctgcacgcc
     5461 gcgccgaaca ccgcaaaaac agcccagctc agcatggtga cgaagatgcc gttttccgcc
     5521 actttattgg cggcttcaaa gtttttttca cccaaacggc gggacaaaag cgcgttgatg
     5581 cccacggcgg tgcccacgga caccgcgatc atcaggttct gcaccggaaa ggctagactc
     5641 acgctggtca gtgcgttttc gctcaccatc gagacgaaca cgctgtccac cacgttgtac
     5701 agcgcctgca ccagcatgct gagcatgatg gggaacgcca tgctcggcag cagcctgttg
     5761 atgggctgcg tgcccatgat attctctctt ctttcttcca tttttcacga ttctcctgtc
     5821 ttttttacaa taccttacta ttctaacata caatataata gaagaaaacg gggaacaatg
     5881 ccggaatggc agcgttcccc gttgcagtat gtgcgctttt tgtacaaaaa tcctggtgaa
     5941 atttttcacc aatattccgt catttgtcca tgagccggat atcggtgccg cagaagcaca
     6001 gtgtttcgca gctgcccagc aggcgggaaa ccaccttttc ggtataacgg cgctgcaggt
     6061 cggcgtcgtt gacgatgttg ctggtgtaga tggtgggcag gcggcggcac acgcgggtat
     6121 tgacaaggct gtacaggcag ctgtttacat aaggggaaat atactccgtg ccgaggtcgt
     6181 ccagaataag cagctccgcg ctttgcagcg tggccatggt gtcgccctcg ccggaggcga
     6241 agcgctcgcg ttcgatggcg tcaaacgcgt tctgcgcgct gacatacacc acgtcgcagc
     6301 ccttttgcag cacggtattg gcgatggaga gggcaagatg tgttttccca agccccgcgc
     6361 tgccgaacag gtacaggctg gtactctccc gcggggagaa atgcgccgca taatatttgc
     6421 agtattccag tacctgggcc atatgctgcc gggccgtgag gccaagctcc ggcaccagtg
     6481 tatccggata tttttccagc gcgaacgtct cgaaggagca cagcgagagc ggggaggagg
     6541 cgttcacctg ctgctggcgc agctgccgca ccagcgcgtg cacgcattcg cacacctcgc
     6601 cgccgtcgcg cccggtgtcc tgacagatgg gacaggtata cgcggggaca aggcgcgcgg
     6661 caatatccgg gtgagcggcc agcagcgcgt cccgctccct gtcgaactgt gcggcgcggg
     6721 ccagcgccgc gtccacggcc gtccgctccg cgcccatggc tgccagacgc gcggcctcga
     6781 tgccggctgc ggtgcgtccg ttttccagcg cggccagacc gggcactgtc tgcgcggctt
     6841 gagcggccgc ggcgcgggca gctgtaacag cacgctgccg gcggcccgct acgatgctct
     6901 gcgcccggcg gtaaagctca tccctgttca ttgtgttttc ccttcctgcg gcatgcccgg
     6961 cacacgcggt gcgcgccgcc gcccgccgcc cagaatattg tgtttcacgg cgggcgcggc
     7021 ggcctgcacg ttgctcgagc ccatggcgct ttcggccatt acgtcgcgca cggttttgta
     7081 gcccttggtg taccatgcgc gcaggatgcc gttgacatat tttacgcttt ttttctcgcc
     7141 cgcgtaggcg gcagcctcgg cgatcatgtc cggaccgtaa ccgtagcttt cgtaccagtc
     7201 cgcgatggca cggctgtcgg ctttggtgaa cttggcgtcg gcaaggccca gcagctttgc
     7261 ggtttctttt tcgtgggccg cgcgttggtc cagcagacgc agatagcgct cggcggcttc
     7321 gccggaatcg ataccctccc gctgccagcc cagcagcgca cgctcgatgt accgggcgct
     7381 gcgcttgccc agcgcggcaa aatgggccac gcccagcagg atcatatcca caggcatgcc
     7441 gtcggaaagg tacatcgaga cgagaatgtt ggtgtccgcc tgcgtaatga cgccgcccat
     7501 cagcgcctgg cattcctgca ccaatcccgc aatggcgggg tcgccctgtg ccgcgctggc
     7561 aacctcaggc gtggtcaggt gtgcgcgagg cgcgggcgcg gcgtcaatgc caccgcgggc
     7621 ctgcggttcg ccgcagctct ccaaaaggcc ggcgcctttc cagtaaacaa gcgccgtctg
     7681 cgcccgctcc gggcttttca ggcgcagcgc cgcggcaatg cgcgcggggt cggtttcgcc
     7741 tgtcgccacg acaaacagtg ccacgcgcag ccagtcgtcc tccagctccg gcagcttggc
     7801 aatcaccagc tgcggcacgg ccacgctgtc ccctttgttt tccacaagac ggtatgccat
     7861 cgtttggttt cttcccttca aaaatcgctg acatccgcgc catttcaaac gcaaagaggg
     7921 tgctgccggc tttgtgcgca gatatcggct tttattgtag catatttttg cccggtttgc
     7981 tacaataaaa gcagcgcaga cgttccggac ggaacagccg gacctgcccg gctgcccgaa
     8041 aaagaacgcc gccgccccgg ccgcgcaaag agctgcggcc gcgattgcgg ggaagacggg
     8101 ctttccgtgg taaaaaacgc cgccgccccg gctgcgcaga gagattcagc cgcagctgcc
     8161 ggtaaaacgg gcttcccatt gttaaaaagg agggcgctta tggatgtgcc gaccatcccg
     8221 gccaggagca ttgtcatcaa aaacaaaagc accgcctggt tcggcacccg gtatacgatg
     8281 aatatttaca ggggctgcag ccacggctgt atttactgcg acagccgcag cgagtgttac
     8341 ggcgtgcagg attttgatac cgtgcgcgta aaatcggacg cgctggccgt catacggaac
     8401 gaccttcgct ccaagacgca gagcggcgtg gtgaccacag gctccatgag cgacccttac
     8461 aatccgtttg aaaaggagct gcagctgacg cgccacgcgc tggagctgtt ggatgcttac
     8521 ggcttcggcg cggccgtcgc cacaaaaggg acgctcatca cccgggacgc ggatatcctg
     8581 cgggagatcg cgtcccattc gccggtgctg tgcaagataa cggtgacgac ctgcgacgac
     8641 gtgctggccg cgaagctgga gccgcgcgcg ccttcgtcct cggcgcggct ggcggctgtg
     8701 gagaagctgg cggggcaggg catttttaca ggcattctgc tgatgcccgt cctacctttc
     8761 ctggaggata cgccggacgg ggtgcgcagg ctggtgcggg cggcgtacaa cgcaggggcg
     8821 cgctttgtat atccggcctt cggtgtgacg ctgcgccaga accagcgggc ctattttctg
     8881 gacaggctgg acgagctgtt ccccggcgca gggctgcggg cgcggtatgt gcggcagttc
     8941 ggcacacagt atgaatgccg cagcccgcgg gcgcgggagc tgtggcaggc gtttacggga
     9001 gaatgccgcg caacgggcct tttgtacggc atgcaggata ttgtggcagc gtatcagcag
     9061 ggctacggca gcggacagct cagcttttta tagcggaacg gcggcctggg cttcgggcgg
     9121 cgtgcccgca gagttttttc agccgggctt ttgcggcggc cgcgggatat ggtaaaataa
     9181 ccgcaggtcg tttttaggct gtccaaaagc tttgagcggt gaaaggcctt gcaaatacta
     9241 taagtttgtg cccctcaagg tgctttaaat aagaacaatt tttgacactt tggcgcccag
     9301 atggcgctcg aatttcccat agagaaataa aacgatacgg acaaatataa gggagaacca
     9361 tccatataaa tggaaaaagg agaggggaac ttgtatatgt atggtctgac ccgatcgaat
     9421 aaattaacga acatgaagtt gcaaaggatc gatgtattat gaaacataaa gtgaaagtaa
     9481 cggtaataga taaaaagcta tatccagaat tgcagcaaca gtattgcgcg gaccctgatt
     9541 cgggttcttg cccttgctat aatgttggcg atgaatttgt tttttatcgt aatgatgaaa
     9601 gagatgattt ctggcatatg gggttgaata ccttaacgaa aacaacgtcg gatcctgata
     9661 cagtggccgg cggacccaaa atgccacatt gttcagaagc ctgggatgca atttcaagat
     9721 acatctacac agggctgcag ggtggctcga ttatgaaaga ttggatgaaa gaagagaata
     9781 caatgatagc ttgttgctca gatggaacaa ggcctgtaat atttaagatt gagagaattg
     9841 acgaaaaata agtacaattc caagcagtac ttatgtgtat tgtcttattt gtggtgctaa
     9901 aatggtggct cgagcctgca aaaccaactt atacgacgat atgaaaatac aggcgttttt
     9961 ctcgcttcaa taaacatttg caggaagtta taaggcgatt tccgtctcta taatcaataa
    10021 aaaatagccg aagattgttt ttcagatcct gcgggccgtc cccggcccgc ggctatcaag
    10081 ataagctttt tattgcggag caataaaaaa cagcccgcgc ctgtttttgg actctgcggg
    10141 cggtctaaac agcggcagtg ccgccgcttt tatgtgcagc cgggccgctc gggattttaa
    10201 gagcgtgatc cggggggcca caggagggtt tcccggcttt gcgaagcaaa gccgggaaag
    10261 acgagccgct tcggcgcaga gcgccgcggc cacagcggca cagcgccgcg cgggaggaat
    10321 tttatgagaa aaaaggcgag cgtgcgccgc acgttttacc gttcattctg tctgctgatc
    10381 gtgctgccgc tggtggcggt ggctgccgcc ggctcggccc tgctgtaccg caagctgctg
    10441 gccgacgcac gggaaatggt gcaaatgcgg caggaggcgg tgtcttccgc gctcacacag
    10501 gacgtgcggc aggcttcgct gcagctggcg catttccttc tttcagaaga cagcgagggc
    10561 cttgctctgg tggacgagct gcccgcgtcg tccagccgcc ggtatgaaac ggccgcccgg
    10621 ctggagagca tttttgatta ttaccgcctg acgggccgcc gcctggaggc gctgcacctg
    10681 tatctgacgg acggtacgtc ctatcagctg tatacaggcc tggccgtgcc gcagaaccgg
    10741 gtgaaggaag ccgcgtggta ccgcgccgcc cggcagaggc cgggggaggt ctgtgtgggc
    10801 gtggcggagc cgggaacgct gctctccggc aaaaaccggc gcggagatct gctgctgacg
    10861 gcggcgctcc aggtggagcc gggatatggt gggagcgcgc tggaagtggc atgcctttat
    10921 tttgagacgg acgcggagga gatgttggaa agctacaaca gtgaagcagg cgcggtgcgg
    10981 atggcgctgg ctctggatgg ggcgttcctg gccggggacg aggcgctggc accggccgta
    11041 gagcgtttcc tggggccgga gggcgttccc ggcggcggaa tggattgggg catcgccaca
    11101 caggtgcgca acacaggcct caccgtggtg tctctggtgg acaaccggaa gctgatggcg
    11161 ggctacgaac ggacgctgtg gctgtgcgcc ggagcggtgg ccgcgatgct gggctcgtac
    11221 gcgctgtttt cggtgctgtt tctgcggcgc atcgtgctgc cggtgcagca tctgaacgag
    11281 ggcatggagc atctgcagca gggggatnnn nnnnnnnggg ccaccttgag atgcgccagc
    11341 ttctgcagcg ctataacgat accggtgaca gaatgcaggc cctgatcgag gagaaccgcc
    11401 tgcgggagga gcagcgctat gccgaggagc tgaaggccct gcaaagtgag atcaaccctc
    11461 attttctgct gaataccgtc aataccatcc gttttatggc ggaccttgcg cgctttgatt
    11521 ccatccgtga catggcggcg gacctgatgg agattctgcg ctgtatgctg cggactcccg
    11581 cagagcgcta tacgctggcg gacgaggcga aaatactgga agcgtatatc catatcatgg
    11641 aggtgcggta ttccggcagc ttcagcttcc ggtgcgcttt cagccccgag agcctggcgt
    11701 gccgtttgcc caagctgctg ctgcagcctc tggtggaaaa cgccctgctc cacggcctgg
    11761 agggctgcga ggacggcgag gtcagcctgt ccgggcgcac ggaggacggc gttttgtacc
    11821 tgagcgtctg cgacaacggt gtagggatgg acccggagcg gctggccgcg tgcctgtccg
    11881 gccccggtga gggaaaagcc ggctcatcca tcggcttggc gaacgtgcag cggcgcatcc
    11941 tgctgcaata cggtgcgcct tatggcctgt gggtggacag cgcgccgggc cgcggcacct
    12001 gtatttcgat cacgctgccc gctgggagcg ggcgggaagg aggcttgcca tgctgcgcgc
    12061 aataatcgtg gacgacgaga tcctggtgcg ggccacgctg gaaaaaatgg tggactgggc
    12121 ggccgagggc tttcagcttg cgggctgcta cgccaatgga caggccgcgt tggaggctat
    12181 ggagcggtgt ccggcggacc ttgtgttcac agatatcaaa atgcccgtct gcagcgggct
    12241 ggaattcatc gacggcgtgc atgccctggg ccttgcgccc cacatcgtag tgctcagcgc
    12301 tttcgatgaa ttccctctgg tgaagcaggc gttcaaaaaa ggggcggcgg actatgtgct
    12361 caagcaggag atcgttcccg cccgcatgct ggagctggta cgcgaggcac gggcggcact
    12421 gtgtgccggc ggcgatgcgc cggcacccgc cccaggcgcg gcgccgctgc gtgataccaa
    12481 tgccattttg caggacgtga ttttccacaa cgcgctgccg ggggaactgc cagggctgga
    12541 gcagggctat gttgtggcct gcttctttct ggatgaaata taccgggaga tggcgcgtct
    12601 gggcacggac gtgaatgcta cgctgaccca gcccctgaca aagctggtga tgcagatgcc
    12661 gcagttccgc tccaccgacg gtttttattc ctttgacatg tcgcgccact ttttgtttta
    12721 ttctctggca cagcccggcc gtacggtgga aaaggcccgc acctttctgg cgcaggtgca
    12781 gcgcgcatgg aagaattaca tgaatatttc ctgtacggtg ggcatggccg cgccccatgc
    12841 cgagccggag acgtcgtttt acacggtgct ggagcaggcc gagaccaaca ccaccctgcg
    12901 ctatgtgctg ggtccggggc gtatctatga cgaaagctat tatccgcaat tcgaccccgt
    12961 gacggcgctg cgccaggcaa aaagctgcat gccctttatc cgtgccgtaa tggaggccga
    13021 tttcgcgcag gtggagcagt ataagaacga gctggtgggc tatatgcagg acctgccgct
    13081 ggagcagggc cagtctctgg cgttgctgta cctgtacaat ttgtattatg agatgggctt
    13141 ttacgatatg caggtcgcct acaagctggg gctggaccac cgcctgtacc aaaggctgcg
    13201 cgccatcgag acccagcggg acctggtgat ctatttcacc tcggtgctgc ggcgcatcat
    13261 ggaatatttt gaatccaact acgaccagca gttcccggac cccgtcctgc gggccaagcg
    13321 ttttctggac gacagctaca tgcgcgcgga cctgacgctg ggcgaggtgg cgcagcgcag
    13381 cggctacaat gaaaaatatt tctgcacgct gttcaaaaag cggttcgacg tatcctattc
    13441 cgactacctc acgggcctgc gcattgccgc cgcgcgggag ctgctggaga aaaccaatat
    13501 gcgcatgtac gaggtctgcg acgcggtggg ctacaacagc gtggagcatt ttctgcgtac
    13561 gttcaaacgc gcgacggggc agacgccgct gcaataccgg aaggaaaatg taaaaaaaca
    13621 tcaataaaac aaactccgtg tcatttcaag gcgcggagtt tttgtgttat gctcatagta
    13681 tgttttacag agtgcgcggc ggcacggcgg gcgtcttgcc gccgcggcac agcttaaagg
    13741 aggaaaatca tgatgaaaaa agttttggca ctggctctgt gcgtgtgcat ggccgccatg
    13801 ctgctggcgg gctgcggcgg ggcgggcagc tcttccgccg ccgcttccgc ttccggcagc
    13861 gccgctgcgg gcaccagcgc ctccgacgcg ggcgcgctgg atccaaacat caaagcgacg
    13921 ctcacctttg ccac
//
LOCUS       DAWBSB010000026        13768 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1091_length_13768_cov_3.133888, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000026 DAWBSB010000000
VERSION     DAWBSB010000026.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 13768)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13768)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13768
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1091_length_13768_cov_3.133888"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2570..2579
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3067..3076
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3791..3800
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5803..5812
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8381..8390
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2628 a         4415 c         3813 g         2862 t
ORIGIN      
        1 tgtagcaaaa cagccccgtg ccgctgaaca gcctgctttt caggcgcaac gctccctttg
       61 ccatggcgct gacaaggccg tccggcgtca gcagcagcaa agcgcggtca gcttcgcccg
      121 tctttgtcgc ccgcagcacc aggcccattg tcgtcgtccg cattttgtgt ctcctcctgc
      181 gtgccgtgct gcttaaaggc aaggtagtcc tctacctggc ccgtactttc aaacagtttc
      241 cagtagttgc ttgtttccat ggtgaacacc tccgctaaca ggtactattc accattgtag
      301 cgcgcatata agcggaaatg ttgtccaaaa acagggaacg cggcggattt atttctcctg
      361 cccggccctg cgcttatttt tgaaagccga aattgtttaa cagaaaatcg ttgtcgcgcc
      421 agtcgtcctt gatctttacc cagcactgca ggttcacccg tgcgcccaga aactcctcgc
      481 actccacacg cgcctcggac gcgattttct tgagcatctg cccgccctta ccgatgatca
      541 tacccttatg gctctttttt tcgcagtaga tgtttacgtc gatgtcgatg atatccttgt
      601 ctccgcgttc cttgaagcgt tccacggtca cagccgtgcc gtggggaatc tcgtcccgca
      661 ggttcagcag catcttttcc cgcagcacct ccgccacgag agccttttcc ggcatgtcgg
      721 tgtaggcgtc gtccgggaaa tagtgagggc tttccaccgc gaaaggcagc agtgcgtcca
      781 gcagttccgt acagcccctg tcctctgaca cgcacagcgg cagcacgccc cggaacacgc
      841 ccagctcttc cagaaactgc atgcgcacgg caaggtctgt ttcttttttc agcgtatccg
      901 tcttgttcac cgccgccagc gcaggcactt ttttcacgcg gatgtcctcc accagcgcct
      961 gctcggcttc cgtcagctcg ccgtacggtt caaacagcat taccaccgcg tccacctcgg
     1021 ccacggaatc cgccgttgct tttgccatgc gctcgcccag cctggtgcgt gggcggtgga
     1081 tgcccggcgt gtccagaaat acatactgca ccgcaccgcg cgtcaatata cccgtgatgc
     1141 gcgttcgtgt cgtctggggt tttgcggtga cgatggccac cttttccccc accattctgt
     1201 tcaaaagaga cgatttcccc acattggggc ggcccacgat ggccacgaag atcgattttg
     1261 tctgttcctg ctgcaattat ttgccttcct tccgttttcc aaaaatgaac tgccacgcca
     1321 cggcaagaga cgcggccagc agcaccgcca gcagcggcct tgccgcaaag aaagcgaaca
     1381 tgcgccggaa cgccgcgaca tcccagaaca ggacagcgcc gcacaccgcg cacccgatgc
     1441 tgaataccag caccgcgccc gcggccatgt ctttcaccac gcccgcagtc atgtaccggt
     1501 ccggcgcggg gcgctccacc gtgcgctcca ggctggagtt gacaagctcc agcgccaaaa
     1561 ccccgcatac catgagcgtg atgagaatgt actctgtttt gccaaagccg taaaacagcg
     1621 aaaaaagata aacgtaaaac gcggcgcaca ggtggaaccg catattgcgc tcctcgcgca
     1681 ccgcggtgcg gatgccctgg gcggcgtaca gaaagctgtc cacgaagcgc ggcttcatct
     1741 cagtccacct ggctgtacga aaccgtgcgc ggcaggccca gctggatgag cgccgcctcc
     1801 tccttttcgc gcatacgcac ggcctccagg ccgcctgctt cgtggtcgta acccaacagg
     1861 tggaacatgg aatgcaccgt caggaacgcg acctcccgct ggagggaatg gccgtatata
     1921 ttggcctgct ccatggcccg ttccatcgag atgacgatat cccccagcat cgcggcgccc
     1981 gtctcctgat tgatgtcgta cttgccgtcc tgccccaacg gaaaactgag cacatcggtg
     2041 gcctggtcct tatcccggta ctgcttgttc agctgctgga tctcggcgtt gtccacaaaa
     2101 gtgacgctga tctcggcagg gccctcgaaa tgctcagcct ccagcaccgc gtggcacgag
     2161 cggcggatga ggatgcgtag cccggacggc accttcaccg tgttctgctt gttggtgata
     2221 tatactttat gagacatcaa aaacacatcc tttactttct gtttctcgcg gcctgcgctt
     2281 tgtcgtaggc gcgtatgatc tcctgcacca gcctgtggcg cacaacgtcc ttttcggaaa
     2341 aatggatcat ggcgatgccc tcaacgtttt tcagaacgcc cagcgcgtcc acaaggccgg
     2401 agcgtgcctt atccggcaag tcgatctgcg taatgtcgcc cgtcaccacg accttggagc
     2461 cggcgcccat gcgtgtgagg aacattttca tctgttcggg actggtattc tgcgcttcgt
     2521 caaggatgat gaaggcatca tccagcgtac gcccgcgcat ataggccagn nnnnnnnnnt
     2581 tccagcagct tctggtaatt ttccgcgccc agcatatcga acagagcgtc atacagcggg
     2641 cgcaggtacg gatccacctt attctgcagg tctccgggca aaaagcccag cttctcaccg
     2701 gcttccacag cgggccgcgt gagaatgatg cggctcacct cccgtgcctt gaaggccttc
     2761 acggccatgg ctacggccag atacgtcttg cccgtgcctg ccggccccac gccgaaggtc
     2821 acggcatttt tgacaatggc gccgatatac gctttctggc ccaacgtctt tggccgcacc
     2881 ggacggccct tggctgtgat ggcaataaaa tctcctgtca ggtccttcac gcgcgcttca
     2941 tccccgctgc cggccagcga gatgcagtaa cgcaccgtct gttcttccag cggcgtaccg
     3001 cctgcccgca gcatcagcat ggcgtccaac gccctgcggg cacgctccac atcctcttct
     3061 ccgcctnnnn nnnnnncggt gccgcggcat atggccgtta cgccaaaggc ctgctccaac
     3121 agacggatgt tctcatcgca atttccaaaa atggcaagtg ccgtatccac tgaatccact
     3181 tcaagcactg tttccgccat tccaacacct cttatttttt attgtacgct gcaagttttt
     3241 gcagcatatt tcagcattcc ccaaaccgca tgcccgcaaa cgcctttgcg cgtttcaaca
     3301 ggcgcaaagc ctcatgcgcg ccagcgcatc cgggcatgtc tgcgcatgcc cgcgaccgcg
     3361 cagcggtagc tttgagacta ttataacgcg acgccgcaaa gcggctgcaa caaaacaacg
     3421 ccatgccgtt gttttgcgcg tttcaacagc cacaaagccc catgcgcgcc agcgcatccg
     3481 ggcatgtctg cacatgcccg cgaccgcgca gcggtggctt tgagactatt atagcatatt
     3541 tatcaaaatt taaaagagga ttttgtacat aagttctaca aatttttctg aatttttcct
     3601 atgaatctgc accaatcgtg ctgtccggct ccatggcctc cccctcatag ggcaccattt
     3661 tcccgatatc cgccagcatg cgcacccgca gcgtcaaggt caccgcaccg tctgcttcct
     3721 cagcagacgg cgtttcttcc agcagctcat agtcgccaaa ttccgcccgg atggcgtcca
     3781 gaatgcgtga nnnnnnnnnn aatccgaaag gggctgccgg gcggcgcagc acgcgctggg
     3841 acgcgttttc cggcacatca agctgtgcat aaagcgaaag ttcaccccag ggaaggtaca
     3901 gcttgtaata gcttttgctc acggcttggg gcagtacagg ctccacatgc agcggctggg
     3961 tatatacata gcttttttcc acctccgcca gcacctcggc ctgagcatgg gaataaagcg
     4021 gacgttcata ctggctcagc gtcataccgc tgacaagcac ctggccttgg gccacatact
     4081 ggcccggcac cacgcgggga tagccgtcca ccagctccag ccgccgtatg atgccgtcgc
     4141 tctttgccac tacgttggtg acctccgtgc cgatgggctc cggcaccttc tcccgctgtg
     4201 ttttttccac caccagacgg ccctgcacaa aattcagcgc gacccagccg tattcttccg
     4261 accccacaaa cagttcgccc gccgcgcgca ccagcttctc attgttttga aacgcacctt
     4321 catagattcc cttttcaaac agctgcgcgc gggcggatgc ttcctgctcc ggcgtaaaat
     4381 tgtaaaagca gatgttccag ataaggttgc ggcatatgca cagcagcagg acgcacagca
     4441 caaggcccgc cagtatcccc cagcggtgac ggtatgtgaa cagcgcgaag tatgcgccgt
     4501 atttctcccg cacgcgcagg cggcaccggt tccggcgcgc atatttgtgc atgcgcttgt
     4561 aatcccgcag aggcactgtg gccagatagc ccgtttgtgt ggggcggata tgttcgaccg
     4621 ggacgcccgc gttcacgcag tcgtttaaaa agcgctcgcc gcgcccgcct gttacctcaa
     4681 aattcacgct tcccatcgcg taccgcgcca acctgttcat caaccgccct gcactcctcc
     4741 gtaaccaaaa tggatggaaa acacctgccc tcgcaccgtg atgagcgttt tttggtaagt
     4801 gtccaccacc agctcgtttc cttcgatcgt aacgctcagc tcgcccatgt ccagcttgag
     4861 tttttcggca tcgtactgca ggatgctccg gcagttttcg atttccagcg cgccgccgca
     4921 atccacatgc agcaccggcc gcccgtacag cacggtctgc accgtctgcg ttccaaatcc
     4981 catcgcgcgg cgaaaggagt agcgctcgcc cggcttcttt ttcctgcgca tcagccgccc
     5041 tccctactat ttgattccgg ttcaatcata tatatcaggg tggagggacg gtttatgaat
     5101 attcttaaaa taaaacgcgc tctggccgag cttgtgtgga ctggcgcgct gttgtttgcc
     5161 gcagccagcg tgttccttcg cccgggggcg gcggcgggcg gcgtactgcg cgggctgagc
     5221 gtatgctacg agacggtcat ccccgcgctg tttccctttc tggtgctttc ccgtctgctg
     5281 ttggagagcc gtgccgcaag cgcgctcggc ctgctgctgc ggccctatac ccgtttgctt
     5341 ggcctgcaca gcccaaaggc ccccgcagcc atgctgtgcg gcgtgctggg gggctttgcc
     5401 ggcggcgcaa aggcggtgga tgttctgtac cgcacaggcg agttaactgc cgctgaggcc
     5461 gcgctgctgc tggtatgtac cattggctcc ggaccggggt tcgtcgtcag cagcgtaggc
     5521 gcgctgatgt tgggcagcgc gggcgcgggc tggctgcttt taggcgcaca gctgggggcg
     5581 tcacttgtct gcgggctggc cgccgcactt tttgttcgcc tgcgcaaacg tggcagcata
     5641 gcgggccgtt tccgggccgc cgtggcctgc gcagcgcaaa cgggcaaagg catgcccgcc
     5701 ggccgctgtt ccgtcccgca aaaaacacag ccggacgccg ttccgtctca gacgggtttc
     5761 gttcccgccg tacgggactc ggtcaacgcc atcgtgatgc tgnnnnnnnn nngctatgta
     5821 acgctgttct ctttttttgc cgccattgct gtccccacag gcgcagatgc gtccgtccgc
     5881 ttttgggcca cgctgcctct ggaggtcacc agcgcgtgcc gcgccgcctg cgagactgca
     5941 tccgcctggc gcacacagct gtgctgcgcc gcactcagtg taatgggcgc ttccgtcttt
     6001 ctgcaggtgc gtgcgctgct tgccccccgt ataccgctgc tgccgctgct gctgtcccgt
     6061 ctgctgcatt tgccgctctc gctgctgctg ttcgggcttt tggcaaagct gtttcccatg
     6121 gcgttgtccg tgggcgctca gtgggacgcc agccctcttc tggccgtgcg catgccgccg
     6181 gatgtaatgc tgatattatt cgccatggcc gcgctggcct gcggtgcctt acccgtgccc
     6241 cggtatttac gccgccgctg aaaaggggta taatcaatgc agaccgcaca aatgcgcccc
     6301 ttccgcagcg cggccaagct ccaggaaagg aattcactcc catgtttaca aaaccgctgt
     6361 tcggcaggga tatccccccg gcctgtgaat actgttccca cgtggtccgt gctgccgatg
     6421 gttcgctgcg ctgtacccgg cgcggcgcag tgcagccttt tgaccactgc cgtgcctttt
     6481 cttatgaccc tctgcgccgc gtgccgcgcc gcaggccaca gctccccacc ttcagcccgg
     6541 atgattttaa gctgtaagga ccgctgcatt tttacgcgtt ttccgcacaa catgaaaaca
     6601 ggcccgcgcc tgtatcccgc ccggcggcgt gaaccgttcg gcagggtaca ggcgcgggcc
     6661 tttctgtttt cagctttcgt catcttcccg ccagcatcag cccgaaagcc gccagcagcg
     6721 cggcgctgca cgcccacacg gcgtgtacgc cccaggcgcc ggccagcacc gcacccgccc
     6781 aggctcctgc acagaacacg aggatgaccg cacagtaccg aagcgccgca cggcccgccg
     6841 ttttgtcgcc gtgtgcccag gcgaacacat gctcgcctgc actgcgcaaa ttgcccgtgc
     6901 acatcgttgt ggcataaggc aggcccctcg tgcgccgaaa actgttcacc tgcagagagc
     6961 agatgaacga aaccgtcaca ttcaccacag cgtccggcac gctccgcggc acgaatccca
     7021 ccgcaaaaac cagtattgcc tccgccagca gcaccacgtg ctgccaagcg atatgctgcg
     7081 cgttcgtcag gcgcgccttg agccattccg tgacgatcac ccctgccaaa aacgccgcta
     7141 tgggcagaag ataataaaag gcccgcccgt cccgccgggc ggcgtacagg gccatcagca
     7201 ccatattgcc tgtctgcgcg ttggcgaaca ctcctccccg cagaagatag gtatatgcgt
     7261 ccagaaagcc gccggtaaaa gccaggcaca gccccacacg cagcatctcg tgcgccgggg
     7321 cgcgctccaa ggtttcgcct tcacgccgca gaaatgcggc aatggcatgc agcatactgt
     7381 tctcccctct ccggcgggcc gtgcccgccg cttgcagaca tcattatagc accttctttc
     7441 caaaagaaaa gcgccttgaa tatgatattg tttttcgcaa agcttacttg acattttttg
     7501 caaagcaaac tatactaaaa gtgcaaagcc tgctatacaa aggagaacat gtcccatgaa
     7561 gacgaaaatc ccggaactgc gccgtgcgcg caggctgtcg caggaggagc ttgcccaggc
     7621 cgtgggtgtg acgcgccaga ccattacctc catcgaggtg ggcaaataca ccgcatctct
     7681 gcctcttgcc cacaagctgg cgcggtattt tgggcttgcg attgaggatg tgttcgactt
     7741 cagcgacctg gatggggagg aatgaatgat gatggaacag atcaccctgg agcagttccg
     7801 gagaacagtg cgcatacgcc ttcggctgct gcgcgtcttt atccttctgt gcgccgcagc
     7861 ggtgttctgc gcgcactttt ggggccaccg gggcggtgtg ccgggcgctc ggtttcctta
     7921 ttatgccgac ctgctcaccg gcgcgctttc cgcgctgggc atctccgccg cttttatttg
     7981 catccgcctt acaaaaatgc ttcgtgacga cgtgctgctg cataagatgt atacgaaggc
     8041 caccgacgag cgcgaagccc ttttatggca gaaggccgga cggcaggcgt tctgggtcgt
     8101 tgtgcccggg ctcgtgcttg cggcgctggc cgcgggctac tggagcgcca cggtatgcat
     8161 gacgctgctg ggcgcggctt tgttcacggc cgtcaccacc aaggcgcttc ggttttatta
     8221 tatgcgaaaa ttctgaaagg ggcgtccggc atgaacggca tcgaggatcg tgtcacttcc
     8281 ctgctcacct ggggcctgcc cctgggcgcg ctggccttct gcgccagcct tgtgctggca
     8341 tggttcggtg cgccggatgg attctggcgc ggcttttggg nnnnnnnnnn attccacggc
     8401 gaactggctg ttgtacaggt ttgcgtaaaa gccctgcttc gccagcagct cggcatgcgt
     8461 tccctgttcg atgatatgcc cggcattcat cacaaggata cggtccgcag actggatcgt
     8521 ggacagccgg tgcgccacca caaagctggt gcgtccctgc atcagctttt caaacgcctg
     8581 ctgcaccagc atttcggtgc gggtgtcgat gctggatgtt gcttcgtcca gtatgagcat
     8641 ctgcggctgg cacagcatga tacgcgcgat gcacaaaagc tgcttctgcc ccgccgaaag
     8701 gtttccgccg cccggtgcaa tgacggtatc atatccctgc ggcagacgct tgataaagcc
     8761 gtgggcataa gcggctttcg ccgcggcctt tacctcctcg tccgtggcgt ccggcttgcc
     8821 gtaggcgata ttctcgcgca cggtagcgca tttgagccag gtctcctgca gcaccatgcc
     8881 gtacatgccc cgcagcgcac tgcgccgcag tttggcaatg ctgttcccgt ccacggcgat
     8941 ctcaccccgg tccacctcgt aaaagcgcat gagcaggttg atgagcgtcg tcttgccgca
     9001 tcccgtcggc cccacgatgg cgatgcgctg gcccggcatc acatccaagt tgaaatcctc
     9061 aatgagcggc accgccggaa cataggcaaa gtccacatgg ctggccgtca cacggccccc
     9121 gcaggccgtg ggggcaaggg cgtccggcgc atccgggcgt tcggtcggct cgtcgataac
     9181 ggcgaacagg cgctcggcgc tggccacagc cgtctgaagc tgcgtcagca cgcccgtcac
     9241 ctcgttgaag ggcttggtgt actggttcgc atacgtcaaa aagcagctca gctggcccac
     9301 cgtaatggaa cctccaatgg ccgagatcgc gccgataaca cctaccgccg cgtagacgat
     9361 ggcattgaca aagcgcgtgc ccggattcgt cacagaagaa taaaacacgc ttttcaggcc
     9421 cgtgtcgtac aactcatcgc tgatggcgtc gaactgtgcg aaacagcggt cctcgtagcc
     9481 aaaggccttt acgacgctct gcccggcaac catctcgttg acaaaacccg acagacggcc
     9541 ctgcgcagcg ctctgcgcct tgaaaaagcc tgttgtgcgc ctggcaacga agcccgcaaa
     9601 agcaatggac accggcgtca ccaaaaccac gaccagcgcg atgacaggat tcagcacagc
     9661 catcaccaca agcgtgccca gaatggtagc cactcccggc aaaagctgtg tcaggccctg
     9721 cagaagcccc tcggctacaa gatccgcatc gttgaccatt cggctgatga tgtcgccgtg
     9781 cgtgtggccg tcgatgtact tcagcggcgt gcggttcagc acgcggaatg cttcgccgcg
     9841 catatcattg gacgccctgc tggaaatctt gcgggtgcag acattcatta cccactggct
     9901 ggctgcggcc agcagcaccg aaattcccag cagcgccaga tagtacagta cgccgcgcag
     9961 gtccacctgc cccgggccga ggatgttgtc gatggcgcgc cctgtgaaca ccggcccaag
    10021 cagggtaaaa agcacataca gcagcgcgct ggcgcaagat gccgcaaacg ccgcgcggta
    10081 gggccgcacg taggtgagga tgcgtttaac ggttctccgt ttcatgcgcc cacctcctct
    10141 gttacaatgc cctgcgaacg gcaaatttct cggtaaaccc cgcagctttc caaaagcgcc
    10201 tcatgggtgc cgaaaccgca ctgccgcccg tcgtccagca caaggatgcg gtccgcgttt
    10261 ttgatggtgg acgcccgctg agagatcatc acgaccgtca tattttccgt ctgcgcccgc
    10321 agcgcgcggc gcagcgccgc atcggtggcg tagtccagag ccgaggcgga atcatccaaa
    10381 accaggatat ccggctgctt cgccagtgcc cgcgcgatgg tcaggcgctg cttctggccg
    10441 ccggagaggt ttttgccgcc ttcctctacg ggagtatcca ggccgtcggg cttgccgcgt
    10501 acaaaatcgg cgccctgggc cacctccaga gccttccaca gcgcgctgtc cggcgcgttt
    10561 tccgcgccca gctgcaaatt gctgcgcacc gtgccgctga acagctgcgc cgcctgcggc
    10621 acaagaccga tcttggcgtg cagccgccgg gaggtatatt cccgcacatc cgtgccgtcc
    10681 acgcgcaccg cgccgcccgt cacgtcgtag tcccgcgtca gcaggcgcac caaagttgtt
    10741 ttgccgcagc ccgtgccgcc gatgattccc agcgtctggc ccggctgtac ggaaaagctg
    10801 acgccctcca cggcgtcctc gcccgcgtcc ggataagcaa aacgcacgct ttcgaactca
    10861 atgcgcggcg caccgggctt ctccgcggcg ctttggcgcg gctccggcat agagctttcc
    10921 tgtgccagca cttcgcttac gcgcttcgcg ctggccagcg ccttcgtgag gataacgatg
    10981 atgttcgcca gaacgatgag cgccagcagc gtctgcgtca tatagttcac catggcgatg
    11041 atctggccct gctcgatgcc tccgtcctgc gcaaacaggc ccccgaacca gacgatggcc
    11101 acaatggcaa ggtttgcgat aaccgaagtg atggggttga gcgccgccga gagcttgccc
    11161 acgcggatgg taatgtcgga cagcgtgtct cccgcctgct ggaactgttc aatctcgctg
    11221 tcctgctttg aaaaggcacg aatgacccgc gctccctcca ggttttcacc cgcaaggcgg
    11281 gagataacgt cctgctggcg ctggatacgg gtgtattcag gcaggctgcg gctcataacg
    11341 cggtacagca cgaacacgat gagcggtgta gaaagcagaa acaccagcgc gatcttccag
    11401 ttgatggtaa aggccatcac aatggagccg acggtaagga acggcgcgcg gatggccagg
    11461 cggataaaca tgttcacccc cgtctgcacc tgattcacgt cgctggtgag gcgcgtaatg
    11521 agcgagttcg tgcccacaga gccatactcg gcctgggaca ggctgaacac atggcggaac
    11581 aggtctttgc gcaggctgcg gccgaagccc tgtgcgcaca cagccgcaaa atactggcac
    11641 gtcagcgcgc aggcaaggcc caccgcaccc agcccaagca taaacaggcc gtgcgcaacg
    11701 acgtatttca cgtcgccgtt tttaatgccc acgtcgatga tatttgccat cagcagcggc
    11761 acaaacagtt ccagaacggc ctccgtcagc ttgaacaaag gccccagaac gagctgcctg
    11821 cggtaaccgt caaaatagcg gaacagtgct ttcaaacttt cacatccctt ttctcagaag
    11881 cggttttcat tcatagttat tttatcacag cattgcccat tttcaagaaa tggatattct
    11941 tttttccaaa attgaggaat caggcccttc tgccgcgtta caagaacgac gtatttttca
    12001 ggcataccgc aatgcacggc gccccgcagg gcgggaagga gagcgacaga tgtataaaat
    12061 actgcttgtg gaggacgacc ccaccattac cgaggtgctg gagcgccagc ttgcaaaatg
    12121 gggctatgaa gcatctcatg taacagattt ccaacacgtt ctggaaacgt ttgttcagca
    12181 agcgccccac cttgttctgc tggacatttc gcttcccttt tacgacggct accactggtg
    12241 cggcgagatc cgcaaaatca gcaaaacgcc cgtgctgttc atctcctccg ccagcgacaa
    12301 catgaacctt gtgatggcgc tgaacatggg cggggacgat ttcctggcca agccctttga
    12361 cctgaacgtg gccattgcca aaatcgaggc gcttctgcgc cgcacatacg atttcggcgc
    12421 cggtgtaaac gcactggcct gccgcggtgc ggtgctggat ctaaaagacg cctcgctgca
    12481 ctacaacggc caaaagctgg agctgacgaa aaacgagttc cgcattttgc agcttctgtt
    12541 cgagcgccgg ggccgcacgg ttcccaggga ggacatcatg caggccctgt gggaaagcga
    12601 cagcttcatc gacgacaata ccctcacggt taacgtggcg cgcctgcgtg cccatctggc
    12661 ggaaatcggc ctggagggac tcatccgcac acagaagggc ctgggttacc tggtggatga
    12721 gacatgatgg gcgcacggga gcttctgcct gtatttttca gctactgccg ccgtatgctc
    12781 cgggcgttcc tgctggtggc gctgggcggc gccacgctgt tcgtggtgac cattctgtac
    12841 cgcgcgccgc tggagctgcc cctatacaca tgctgccttg taggcgctgt gggagccgtg
    12901 ttcttttttt ccggctttgc ccggtattac cgccggcatg ccgcgctgcg catgctgcgt
    12961 gcccagtgcg ccaccaagct ggcagcgctg cctgcacccc gggatatttt ggagaaagac
    13021 tatggcgata tcattgccgc tctggagcgc aaccgtcttt ctgccgcggc agacgccgcc
    13081 caccgcgaag ccgatgcgac ggaatattat acgctttggt cccaccagat caaaaccccg
    13141 ctggcggcca tgcgccttct gctgcaggaa agcccggata tccctggccg cagcgccttt
    13201 gcccaggagc tgttcaaggc cgaacagtat gtggacatgg tgctgggcta tctgcgcatt
    13261 ggttctatcc atgccgatct ctcgccgcag gaggtccctt tggaggccct tgtgcgggag
    13321 agcgtaaaaa aggtgtccac tctgttcatc catcagaaaa gcacaacgct gcagatcagc
    13381 tccctgccgg gcacggtcct cacggataaa aaatggttct cctttgtgct ggaacagatc
    13441 cttaccaacg cggccaagta cacgccgcgc ggcacggtgc gtgtgtatct ggccaaggac
    13501 tcgcccgata cgctggtcgt cgaggacacg ggccttggca tccgcgcaga ggacctgccc
    13561 cgtatttttg agcgcggctt taccggctac aacgggcgcg gcggccagcg cagtacaggg
    13621 ctgggcctgt atctgtgcca tgaggtgctg caaaggctgg ggcacacgat ctccatccgc
    13681 tccgagccgg gaaaagggac ggcggtgtgc atctgctttg cccgcggcac gctggtggtg
    13741 gaataatcgc ttcggcgcac ggcaggcg
//
LOCUS       DAWBSB010000027        13692 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1103_length_13692_cov_3.722300, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000027 DAWBSB010000000
VERSION     DAWBSB010000027.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 13692)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13692)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13692
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1103_length_13692_cov_3.722300"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2844 a         4251 c         4082 g         2515 t
ORIGIN      
        1 aagacatcgc cctttcacgg cggtaacccg ggttcgattc ccggtcgggt caccagatga
       61 aaaccgcact gtacagccaa aaacggttgt acagtgcggt tttttatgca tttaaaatgc
      121 tgtgacaacg aaatgagaac atcttttatg ccctgttcgt ttccgtgccg gccaaacaga
      181 cccgcatggc aggaagaaag ggcatgctgc tattttttac ttcaataatt ttccgccttg
      241 atttcggtat agctctggga atgcccgcat acagggcaaa cttccggcga gcatgcggca
      301 tacgttacgt ttccgcatac catgcacacc cacacggtgg ggcgcccctt acaaaaaacc
      361 tggcagtcct caatgttggc agccagcttg ttgtaacgct gttcatgcgt tttttcaatg
      421 ttccccacgc gggtgaacag ttccgccagc tcctcaaagc cttcacagcg ggcggtacgg
      481 gcaaattcgg catacatctc ggtccattca tagtgttccc cggctgccgc gtcccgcagg
      541 ttttccagcg tgccgggcac ttcgccgccg gaaagatgct taagccagat ctcggcatgt
      601 tcctgttcat tgcgggatgt ttcatcaaag atgttcccca tctgctcgta gccgtcttcc
      661 cgcgcacgca gcgcatagat cttatatttt gcacaagcgt gcagctcgcc cgaaaatgcg
      721 gtctgcagat ttttccacgt ctgactgtgt tgcagttcca tggtcaatgc ctcccgttat
      781 attgatgtgc cgcaagacgg caaagcgccg tcaaaccatt gtatgcggcg gtacccggtt
      841 ctgaccatgc gcacgggcac cttaaaagga cgggacagaa aaagccctgc cagatcccaa
      901 aaggatctgg cggggcttca gcatttggag cattgttccc ggcaagccgc ctcacatcga
      961 aagaggcgtt ccttcgacac gcgggccgtc ggaatataaa aatacggtct gccgtcgtcg
     1021 gtgcgcagca gagggataac ggtctcctgt ttataagcct gtgtgcgctt cctcaaaatg
     1081 ctcaaaacgc ggcaggaatc tgtgataatg gggcaagatt tccacgtctg tcagacaaag
     1141 cccggtggtg tccaacaagc cggcggattt atcatttccg gtgtgcagcg ggcgacgaat
     1201 gcaatggaac actgcattac aaagcggccg gcgctgcagc tgctcttagg catccagcac
     1261 cagcacacgg atatcctccg ggctgcgggc gagataggac gcgcccgcgc cgcacagctc
     1321 gtccccggtg cggaagcccc acagaacgcc gcagcccgca agcccggcat tgcgggccgt
     1381 ttcgatgtcc acgccgctgt cccccacata cagcacctgc tgcgccgaaa ggccgaaggc
     1441 ggcgcgcatg ccgttcacaa gatgcgggtc gggcttcgtg ggggtgccca gcgtatggcc
     1501 ggccactgcg tcaaacagac ccggaaaata atggctgaca acgcttttgg tgagagcatc
     1561 ctccttattg gacacgacgc ccagttttac gcctgcgccg tgcagcgcgg caagcagggc
     1621 ggggatgccg ggatacggag ccgtagagtc ctccttgtgc gcgtcgtaat aagggaaaaa
     1681 caaccgggcc gcctgtgcct gtgcagcttc gtcccggtgc ccgggaggaa gaaaacgctc
     1741 gatcagcttg ggaatgccgt tgcccaccat gtatttgtat tcttcttccg tatggggcgc
     1801 aaagcccatt tcggccagcg tatgattggc cgcggcggca agatcgccga tggtgttcag
     1861 cagcgtgccg tcaagatcga atatcacagc tcggtacaaa aggatcagct tctttcaaga
     1921 atgagaataa aacgcggtgc ggttcctgca cagcgtcgga aaagcacggt gacgccgctc
     1981 acagcgtgaa cgggatttcc cgcgccagat attgcagtac gccgcgcacg cggtactgca
     2041 gcccgcaggg caggtgcacc cgcacgtcgc accgtccgcc gctgacggaa aatggatttt
     2101 ccagcagcgt ataggaaaga tccggcgtga acacacaaac ctcatgccgg gtaaaaaaac
     2161 gggtataaca tacggacagc gtgcggttgg cgtttttgtg caggccttca cggaaaaagc
     2221 cctccaatga aaccagcagc ccagcgaggg caagcaccac cggtacgaaa agcatgggca
     2281 gaatctgcgg cattgcgtaa gaggccacgc cgcacagcgc caaagaaaga gcgagcagcg
     2341 tgccgggctg ccaaagatac tgcaccgggc ttttgcgtgc aggcatgcac agcggaccgt
     2401 ccggcgggca gaagttgggc agcaggcgct ccactgtccg ctcctcccct tttttataga
     2461 ccagcacggg cagatcgcct ccgcggaagc tgcctgcgga aaggtatacc ggatagcgcc
     2521 gcagaaggcg cgccggcggc gtaacgcaca catcgcaggc ggacacacag gccaccagga
     2581 tacgccgctc gatgagcgtg acaaagccgc ctttgctgat gatgactccg ccgttccggc
     2641 ataccttaaa gcctgccgtg tgcagcagcg catacaaaaa ggtgaatccc gtcacggcaa
     2701 agcccagcgt taccagaaaa gccaggcccg tgggcagata ccgcgccgca aacagctcgg
     2761 cctcatgaaa agtgtccagc gcgatttgct ggaaattctg ccccagaacc tcgtccagct
     2821 gtttcacgcc ccacagggca aaggcgctgc tggtgatgat attggcgctc agcatcacga
     2881 agctcaggcg ttcaaagccc gtgggctcga atacggacgt atcgctgcgc acaggcagaa
     2941 gaacatccgc caccgcggcc gcgtcctttt tatgaagata catggaatag cttttgggcg
     3001 aggcgttcgt tttgaaatac agcgttacac ggctcgcgcc cagcatgcgg cagtacagag
     3061 ggcgggcgat ctccagcgcc gcgatgctgg ctctgccgaa ggtgtgatag ctgcggtaaa
     3121 acacgccctg cgtcaccttt acggcgccgc cctccagccg gaaagccgtg gcgcgccaaa
     3181 gagccagcgc cgcggcagcg caggccagca ggataacggc gtcctgccgg agcgccgccc
     3241 aaaggctgtc caggtcgaac gcaagcagcg cctgaagcat cggtaccagg cacagcagca
     3301 ggccgtaccg cagataatgg accgcaaaga tgatatggaa ccggcgttgc ctgttcacag
     3361 cagcttcccc ccgtgcgtca gcttggcgga aaggcactcc gcgtccgcct tgcgcatacc
     3421 gggaaccaat gtggtgcagc cgctggagta caggaagaac aggcaggtgc ccgtcatgcg
     3481 ctgcagcggc gtctgcacga tgtggcagcc cgcaaggaac cgcagcggga tgcgccgcgt
     3541 tacgggaaac agcaggccgc gccgaagcgt cagatggttg ccgcccacac gcacgcagga
     3601 cgaagccagc cgcggcgcca gcacgccgaa gcacgccgca agccacagca gaaataccgc
     3661 cagcgtcggc gtaaagaaat ttttccagaa cggcaggcac agcatcgcgc ccagtgcggc
     3721 gcaccagccg ctgacgatgg cggcgccgtg ccgcgggaca gaaaattcca tagatcttcc
     3781 tttccgccga aaccgcgagc cgcccgtgcg gggctatttt acgtccgcca ggttccagct
     3841 ttgtgccaac tcgtggaacg ccgtgttttt ggccagcacc acgactttat cgtttaccag
     3901 gatctgcgta ttgccgcggg gaacgatcag ctcaccgtcc cgcgtgatgg aaatgaccac
     3961 cgcatccttg gggatgacaa gctcagccag cgttttcccc gcatgggcga agccctgtgg
     4021 aatgagcacc tcggtgagcg aggccgtccc ccccgcaagg ctgagcagct gctggatggc
     4081 cgccgtctcc acctcgcgct cgatgaggtg cgtcaggtta tcggtagcgc tcacggcaat
     4141 gtccacgccc agttttttca gcactgcggc gtttttgggg ttgttcactc gggccaccgt
     4201 gcgcttgacc ttgaacacct gctttgcaag ctggcatacg atcaaattgt cttcgtcccg
     4261 gcccgttacc gcaaccatgg cgctgcagtg agccacgtcc gcactttcct gcacttcggc
     4321 aaaagtgccg tcgccgcaca ccacggtcag gtccaggctg tcggccacct tgcggcacat
     4381 ggccgggtcg tcctcgatga gcaccggcgt gtggccgtgc tccagaaggg tcttggcaag
     4441 atagtagccc actttgcctc cgcccacgat acaaacacgc atatttgtgt actccttatg
     4501 atgtttcccc gcatgggccg ctatccgcgg cggcgggagt attcctcagt ccgccagttc
     4561 cgcaaacagg atcttatcat ccacatgcag cgtaggctgg gtgttggcaa gttccatcgt
     4621 gccgttggcg cgcagcacgc cgaataccat catgcccgcc cggggcggct gtacggacga
     4681 aagcggacgg ttccactgat gcttcgcggc aggtacagct gaaaaatttg ccgtggagga
     4741 accgaacatc acgcactggt cctcgtcgtc ctcctcctgc agaatacccg ccagcaggcc
     4801 ttgggccgtc agattggtgc cgcacacgat gcgcaggcca aagcgctcgg tgtagacctc
     4861 tttgcgggag ggctcggcca cgcgggcgat gacgtgcttc acaccgaaca gctcgcgcgc
     4921 gatctgcgct accatcagat tgatgttgtc atccttggtg acggcggcca cggcgtcgca
     4981 ggcctcaatg cccgcactgc gcagtacatc gccgtcgatg ggcacgcccg ccacggccat
     5041 gccggaaaat ggcggctcca gttcattgag cagagccaga ttggcggcgt cttcgtccag
     5101 cacggaaaca tcgtgcccca gccgctccag cacatgtacc agcctgcggc ccacgcggcc
     5161 gcagcctacg atcaggatat tcatagcttg tctccttcgg tctccgccgg gacggagccc
     5221 ggaggcccgc gccgcgcgga caatggcgcg gccgtgcggc ggcgctctgc cctcattata
     5281 cccgttcccc ggcggggttg caaccgatac acatttatgc tttgcagttt tgccgcgcct
     5341 gtgctatagt atcagtgtat aaaaacagcg gttttccgcc gcgcggcgga aagctccgaa
     5401 ggcttcatcc gatgccgcag gcgcgaacgg aaaggccgcg gagcgccggc cgggcccggg
     5461 aaagcgttca gcgcccgtcc gcggccttgg ctgtgcgccg tttttggtac attgcggcta
     5521 gttaaaaagt accgcgtcta ttgtacatgg gatacggtgg ggaaaaaagc aaaaaaagga
     5581 gaggaacacc atgcaattga acagcgaatc cgtactgaaa ggcgctgaac gtgcgccgaa
     5641 ccggagcctt ttttatgctc tgggttatac tgatgaagaa atcaaccgtc cgttgatcgg
     5701 cgttgtgagc gcgtacagcg agatcgtacc cggccatgct catttggaca agctggccga
     5761 ggctgtcaag gcgggcgtgc gcatggcggg cggtactcct atcctggtac cgtccatcgg
     5821 tgtgtgcgac ggaatcgcca tgggccatat cggtatgaag tattccctgc ccagccgtga
     5881 gctgatctgc gacagcgtgg agacgatggc gcgcgcccac gggtttgatg gcttggtact
     5941 ggtgccgaac tgcgacaaga tcgtgcccgg catgctgatg gccgcggccc ggttgaatat
     6001 cccgtccatt gtcgtcagcg gcgggccgat gctctcgggc cggcagggca cgcgccacgt
     6061 ttcgctttcc cagatgttcg aggcggtggg gagctacaag gccggcatca tcggcgaaga
     6121 cgtgctggac gattacacca aaaacacctg tcccggctgc ggcagctgct cgggtatgta
     6181 tacggccaac agcatgaact gcctgtgcga agccattggc atggcgctgc ccggcaacgg
     6241 aaccatccct gcggtatacg cggcgcgcac gcagctggca aagcatgcag gcatgcgcat
     6301 catggatctg gtgcgggacg gggtgcgtcc ccgggatatt ctgacgccgg cggcattcag
     6361 aaatgcactg gccacggata tggcgctggg ctgcagttcc aacagcgttt tgcaccttct
     6421 ggccattgcc aacgaggcgg atgtgcctat gagcctggag actttcaatg aaatgagcgc
     6481 gaaggtgcca aacttctgcc accttgctcc ggccggcccc acgcatatcg aggacctgta
     6541 cgccgcgggc ggcgtgcccg ctgtcatggc gcagctggcc gggctgggcc tgctggatac
     6601 aagtctgccc acggtgacgg gcaaaactgt gggcgagaac attgcaggcg cgcagaaccg
     6661 cgatacaaac gccatccgtc ccgcggacga cccgtacagc aaaacgggcg gcatcgccgt
     6721 gatgtggggc aacattgcgc agaacggctg cgtggtgaag cgcagcgcgg tcgcgcccga
     6781 gatgctggtg cacagcggtc ccgcgcgggt gtttgacggt gaggaaagcg ccatcgacgc
     6841 catttacaac ggccgcatcc agccgggaga tgtggtggtc atccgatacg aaggcccggt
     6901 gggcggcccc ggtatgcgcg agatgctcaa tcccaccagc gcgctggcgg gtatgaagct
     6961 ggacaaatcc gtggcgctca tcaccgacgg acgcttctcg ggcgcgtccc gcggcgcgtc
     7021 catcggacat gtggcgcctg aggccgccgt gggcggaaat atcgcgctca tcgaggaggg
     7081 cgacgtcatc gatatcgaca tcccgggcgg gcgtatcggc gtgcgcgtgg acgacgatac
     7141 gctggcgcgc cgccgcgcgg catggacggc tcctgcacct aaggagacca caggctggct
     7201 ggcgcgctat gcaaagctgg tgtcgggcgc aaacgaaggc gccgtgatgc agaaataaga
     7261 ttttccgcac agaaaaaaca gcggccggga agtatccggc cgctgttttt catcccaacg
     7321 attcccccgg ccgcagcccg cgtgaaaagc ggccgcatgg cctgaaaaaa tatcctgtgg
     7381 atgttcttaa gtttccggcg gcggagattg catctgctcc gcgcccgcct gccgcgggcc
     7441 cgccgtgcag ggccgctccg cctccagcac aagctgtgca aagccctgat aggtataatc
     7501 cgcgtccggt ccccggtaag gggccaccgc gttgaaaaag cagctcggca gctcgccttc
     7561 ccgcaggcat ttcccaatgc agggcgtgca gcccctgtcg tggttcacgg ggttcagcgg
     7621 acattttgtg tcccggcagg tgcagaaagc aacgttcatt ccgaaaagct cctttccaaa
     7681 acggcagatg ccgccgcagg ggcgcggccg ttctgtctgg cagaacaggg gctgccgttt
     7741 cccgtgctcc gcgtgttgaa actgtttaaa accggcccgc gtcccgcagg gctttcacct
     7801 tcagtacagc ggctacggtc ttactgtcgg tgatctcacc ggagagaacc atttccgcgg
     7861 ccttgtccag cggcagcgtg aacaccgaga gaaattcgtc cgcgtccagg tgctgtccca
     7921 cgggagaaag gcccttggcc gcatacaggt aaatgatctc gctgcaatat ccgcaggtgg
     7981 gaatgatata ccccaggtcc cggtattcgg cggcggcaag gcccgcttcc tcgcccagct
     8041 cacgcttggc ggcttccaat gggtcctccc ccttttccag cttgccggca ggaatctcga
     8101 tcaattcccg gtccagcgcg tagcggtact ggcgcaccag atagatctca ccctcgtcgt
     8161 tgagcgctgc aacgcccgca ccgccgtgat ggcgtaccac ctcccgggtg ctggtgccgc
     8221 cgttttccag ttccaccctg tccagcgtca cggtgatgac gcgtccctcg aattttgtct
     8281 cgctcgaaag ccgtttttca aaatgtgcca tcatgcatcc tcttcttcgt cgtcatcatt
     8341 ttcggggttc agctccggag cgcgggtgca ttttaccttg cccactctcc ggtcctccac
     8401 ttccagcact gtgaattcca gcccgccgta ttccagcgtc acatcttccc ccgctgcggg
     8461 gatacggccc aagcggtcga tgatcaggcc gcccacggta tcgaaatcgg cctcgccgtc
     8521 ctcgtcctca tcgggcggtt ccaggccgaa ggcctcgaac acttcctcca ggtccgcgct
     8581 gcctgcggca acaaacccgt cctcggtggc cacaaggtcc tcgtcctcgt tgtcaaattc
     8641 gtcctggatg ttgcccacga tctcctccag gacgtcctcc atcgtggcaa gtccgctggt
     8701 gccgccgtat tcgtccacca ccacggcgat ctgcgtgtgc tttaccttaa aatccaccag
     8761 cagctcctgg gcgcggcagg cttccggcac gaacatcgcg ccgcgcatga accggctcac
     8821 cggggcctgc gccttttccg gcgtgtccca aagcgcgaac aggtccttca catacaaaat
     8881 gccgacaatg tcgtccagtg acttgcggta taccgggagg cgcgaaaatc cgtgctccag
     8941 agaaaggcgt accgcgtcgg cggcgggcgt gttttcctcc acgcttacga tctcagtgcg
     9001 gtgggtcatc acgtcgcctg ccgtcacgtc gtccagctcg aagatgttgg tgatcatctt
     9061 cttctggctt tcgtctatca gatcgtgctc ctccacgtcg tccacgaaag agagcacatc
     9121 ctcctctgtt acgtcggact gggccgagag ccccagcgcc ttgaacacca gcttcgcggg
     9181 agcggccagt ggcgcaccca gcatacaggc ggccgtgaag ccgcttttct cctttatgcg
     9241 gcccttcgcc cggccaaggc tgtacagcag cacggccagc gcggccagcg ccgccaggca
     9301 cagaagcgtc caccagggag cctgtgcaaa aatgcgaaga aagccgtaaa caaaaaatcc
     9361 gcacgcaaac acaaatgccg cgcaaagccc ggccatcctg tcggaaaaac caaggatgcc
     9421 gctctgctgc cgctccggtt cgcttgcgga cactgcgccc gcgcggtaag ccgccgcaag
     9481 cgcgccaaac gtggaagctg ttgcgatgag gatactaaga ccatcagggt ccatctgaaa
     9541 aaaactcctt ctttccgccc gccgccggag cgcgggccat agaatgcggg cgcataagcc
     9601 cggatattta ccttataata tactggattg aaagcggcgt gtcaaatgcg gggcgtaaaa
     9661 tccgtgtaaa aggaggcgca tggaggtgtg aaggtgggat ttttttgtaa aaatggccgc
     9721 ggttccccat aaaaatcccc tgccccttgt ttactttccc gcaaaacttt gctacaataa
     9781 tgacgagcgt aatgcagaaa cgatgccccc gcaaaacgcc tgtttctgct cacaccaccc
     9841 cccgcccgga atcatctgcc gccgcggggg aataaagaaa gaaccggagg aagaaaaatg
     9901 ccaagatcca agaaatctat ggacggcaac accgctgcgg ctcatgtagc ctatgcgttt
     9961 accgaagttg ccggcatcta ccccatcacc ccgtcgagcc ccatggccga ccaggtggac
    10021 cagtggtccg ccctgcacca gaagaacatc ttcggcaccg aagtgaaagt tgttgagatg
    10081 cagtccgagg cgggcgcggc cggcaccgtg cacggctccc tggctgccgg cgcgctgacc
    10141 accacgttca ccgcgtccca gggcctgctg ctgatgatcc ccaacatgta taagatcgcc
    10201 ggtgagctgc tgcccgccgt gttccatgtt tccgcccgta ccgtggccag ccatgcgctg
    10261 aacatcttcg gcgatcattc cgacgtatat gcctgccgcc agacgggctt tgccatgctg
    10321 gccgagtcca acccgcagga ggtcatggac ctggctgctg tggcgcacct ggccacgctg
    10381 gagagccgtg tgccgttcat caacttcttc gacggcttcc gtaccagcca cgagatccag
    10441 aagatcgagg tctgggatta cgacgacctg aaggaaatga cggacatgga agcggtgaag
    10501 gcgttccgcg cccatgcgct gaaccccgag aaccccgcta tgcgcggctc ccacgagaac
    10561 ggcgatatct tcttccagca ccgtgaggcc tgcaataagt tctacgaggc cgtgcccggc
    10621 attgtggaaa aatacatggg caaggtgaac gcgaagctgg gtaccaacta ccagctgttc
    10681 gagtactacg gcgctcccga cgcagaccgc gtcatcatcg ccatgggctc catctgcgac
    10741 gtggccgagg aagtcattga ttacctcacc gccaagggcg agaaggtcgg tctggtgaag
    10801 gtccgcctgt accgtccgtt cgcggccgac aagctgctgg ccgccattcc ggccaccgcg
    10861 aagaagatcg cggtgctgga ccgcaccaag gagccgggcg ctctggccga gccgctccat
    10921 ctggacgtta tggccgcgtt ccgcgaagcg ggccgcgagg gcaccatcat cggcggccgc
    10981 tatggcctgg gcagcaagga taccccgccg tccagcgtgt ttgccgtata tgaagaactg
    11041 gcgaaggacg ctccgaagga gcgcttcaca ctgggcatca acgatgacgt cacctacctc
    11101 tctctggagg agaagcccgc gcccaacacg gctgccgaag gcaccgtcga gtgcaagttc
    11161 tggggcttgg gcggcgacgg cacggttggc gccaacaaga actccaccaa gatcatcggc
    11221 gaccataccg acaaatatat ccaggcctac ttccagtatg actcgaagaa gaccggcggc
    11281 gagacgatca gccacctgcg cttcggcgac aagcccatcc gcgcgccgta ctatatcaac
    11341 aaggccgatt tcgtggcatg ccacaacccg tcctacgtca tcaagggcta caagatggtc
    11401 aacgacgtaa agccgggcgg cgtgttcatg atcaactgcc agtggacgcc ggaagagctg
    11461 gatcaccaca tgcccgccga ggcgaagcgc tatatcgcca agaacaacat ccagctgtat
    11521 accatcaacg ccatcgacaa ggccatcgaa attggcctgg gcaagcgcac caacaccatc
    11581 ctgcagtccg ccttctttgc tttggcaggc gtgctgccga aggaagacgc gctgaagttc
    11641 atgaaggaag cggcctacaa gtcctttgcc aagaagggcg acgccatcgt cgagatgaac
    11701 tacaaggcca ttgacgcggg ctttgacgct ttcgtcaaga tcgacgtgcc ggcttcctgg
    11761 gccgacgcga aggacgccga gggcgaccac gagctggccg gcaaccccgc caccgtcaag
    11821 atggtcaagg agatcctgtt cccggtcgac aagatggacg gcgacagcct gccggtttcc
    11881 gctttcgtgg atcatgtgga cggccagttc gagcagggtg cttccgcata tgagaagcgc
    11941 ggcgttgccg tgagcgtgcc cgagtgggat gttacaaagt gcattcagtg caaccagtgc
    12001 gcctatgtct gcccgcatgc caccatccgt ccgtttgctt tctccgccga ggaagttgcc
    12061 gccgcccccg ccgcgctgaa ggtgaagccg atgaccggca agggctgcga gaactaccag
    12121 ttcggcatga cggtctctcc gctggactgc atgggctgcg gcgtctgcgt cgacgtctgc
    12181 ccggcccccg gcaaggcgct gaagatgatg cctcaggaga gccagctggc ccagcaggaa
    12241 gtgttcgact atgctgtagc caacgtgggc aagaaagagg gcatgatggc cgagacgacc
    12301 gtcaagggtt ctcagttcaa ccagccgctg cttgagttct ccggcagctg cgcgggctgc
    12361 gccgagacca gctacgcccg cctgattacc cagttgtacg gcgagcgtat gtatatttcc
    12421 aacgccaccg gctgttcgtc catctggggc ggcccggccg caacgtcccc gtacacccgc
    12481 aaccgtctgt ccggcaaggg cccggcctgg gcaaacagcc tgttcgagga caacgcagag
    12541 cacggcttcg gcatgtatct gggccagaag accatccgcg agagcctggc cgacaagacc
    12601 cgtgcgctga tcgctgttga gtacgcgctg cccgacctga aggccgctgc gcaggaatgg
    12661 ctggacacta tggaggacgg caagctgaac cctgccgccg ccgataagta tattgccgcg
    12721 ctggagaacg gcgtgctgac cgtagaggaa ggcatcgcgt tcctggagag cgccgagggc
    12781 aaggcgaagt tcggcgacaa cgccgcgtcc atgctggagc atatgaagag cctgaaggct
    12841 gccggcaagg ccacctgcga ctgcgaggcc tgcacgcttg cggctgaaat tctggagcag
    12901 aagcagtacc tggccaagaa gtccgtctgg atcttcggcg gcgacggctg ggcctatgat
    12961 atcggtttcg gcggtctgga ccacgtgctc gcttccggcg aagacgtgaa tgtcatggtc
    13021 tttgacaccg aggtttactc caacaccggc ggccaggcct ccaaggccag ccagatcggt
    13081 caggtggctc agttcgccgc ggcgggcaag gccatcggca aaaagaatct ggccgagatc
    13141 gccatgagct acggttacgt gtatgtggcg cagatcgcca tgggcgcgga caagaaccag
    13201 accatcaagg ccatcacgga ggccgagagc tatcacggcc cgtccctcat cattggctat
    13261 gccccctgcg agatgcacgg cgtcaagggc ggtatgacca actgccagaa tgagatgaag
    13321 cgcgccgttg aggctggcta ctggaacatg ttccgctttg atccgcgcaa aaaggcggag
    13381 ggcaagaacc cgttcaccat cgacagcaaa gcgccgacgg cggactacaa ggagttcatt
    13441 gagagcgaga cgcgttacag ccgcctgcag cgcgccttcc ccgagcgtgc gcaggtgctg
    13501 ttcgacaagg cagccgacgc cgcgaaggac cgctacgagt acctgggcaa gcttgccaag
    13561 ctgtacgaat aatcggcaca agcgaagtaa agcagagagc gccccgcacc gaaaggtgcg
    13621 gggcgctctt ttaatcaagg tgaattatac tatcaagtgc aacactttag gggaacaaaa
    13681 agaagtccat ac
//
LOCUS       DAWBSB010000028        13522 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1118_length_13522_cov_3.366303, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000028 DAWBSB010000000
VERSION     DAWBSB010000028.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 13522)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13522)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13522
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1118_length_13522_cov_3.366303"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1514..1523
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6164..6173
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2603 a         4250 c         3858 g         2791 t
ORIGIN      
        1 cggagcacgt gctttactgt gtggcccagg ggatggacgc cgtaaaggac ggccccgccg
       61 tcacctaccg caatatggga aagcttctta tcggcagcgg tacgggcgcg cgcacctcgg
      121 ctgtggacgc ggcggccgca ctgctgctgg aggcgggcgt gccctgcgaa atacatgaag
      181 atattctgta ccgccagtgg agcaagctga tgctcaatgt ggggctgaac caggtatgcg
      241 cggtttacaa tgtgccgtac cggggcgtac agtacccggg gacggcgcgg gacaccatgc
      301 tggctgccat gcgggaggca caggccctgg ccgcacggga gggtgtgacg ctgacagacg
      361 cggatatttc cgcctggatg aaaatgaccg atgctttgaa ccccgacggg atgccctcca
      421 tgcgccagga tacgctggcc cggcgtccca ccgaggtaga gctgttcgca ggcgccatgc
      481 gccgccttgg ggagaagcac agcgttccta cccccgtgaa cgatatgctc taccgccgca
      541 ttgccgagct tgaagccgcg tattgagcat gcggcgcgct ataatcccaa tggtacagag
      601 aaaccagcgg agcctgccct ttttatgggg cacaggctcc gctggttttt cgtctgtatt
      661 ccgcgctgcg gcgctcatcc attggcgggc agcttttccg caaagccggg cacatgcacg
      721 gccggcacct ccatgccgcc gtataccggc actttctcgg ctgcgccgcc ctgcgggggt
      781 gtgccgccgc tttgcccaag gcgttccttg gccacgctca gcatcagctt gatacggttc
      841 tcctggttca cccgcgttgc gcccgggtca tagtcgatgg gtgtgatgtt ggcgcgggga
      901 tatatggtgc ggatcttatt gatcattcct ttgccgcaga tgtggttggg caggcagccg
      961 aagggctggg cgcagataat gttctcaaag cctgtctgct gcagctccat catctcggcc
     1021 gtcagcagcc agccctcacc catcttgttg ccgatgccga tgattccgtc cgccattttt
     1081 ttcgtgtcca gaaacagtgt gggcgcatgg aactggggat gctttttaat cgccgcgatc
     1141 atcagctttt cccgcacggt aagccagctc atcaacgtat cggagccgag ctccaacagc
     1201 ttgctgccgc cgtacaggcg cacggtctcg gccatgttgg caacacagta ctgcacaaaa
     1261 cccatcaggc ccggcaggtt cacctcgcag tcctcgccgt ggaggaactg ctccagattg
     1321 ttattgccca acggtgaata tttcacatag atctcgccca cgacacccac tttcaccttg
     1381 ggcgtatagg taacgggcac cgccgcgaaa tccgccgcga tagccgcaaa gttcgcagcc
     1441 atatcctttt tcgagaagcc ctttttttca tcgaactggc tcgccaccct ccgcgtccag
     1501 gcagccacaa gcgnnnnnnn nnncgccgtg cccggatcgt tttcataggg gcgcgtctgg
     1561 ttggcaaggc tcatcagctc gtctccgtaa aacaccgccg caacaatctt tcggatgagc
     1621 ggaagcgtca actgaaagcc gctgtccttt tccaggccgg aaaaattcag gctggccacg
     1681 ggcacctgag gaaagcccgc tttcaccagc gccttacgca aaaggtggat gtagttggac
     1741 gcccggcacc cgcctcccgt ctgcgtgata agcagggccg tgtgatccag atcgtatttt
     1801 ccgcttttca gcgcattgat gaactgcccg atgacaagaa gcgcagggta gcaggtatcg
     1861 ttgtggacat atttcagtcc ttcctgcgcc acggcatggc cgccggagcc aagcacctcc
     1921 acatgatagc cttcgtcctc catggcggcg cgcatcatct caaaatggat gggcgccatg
     1981 ttggggatca ggattttatg tgtcgctttc atctccggag tgaatttggg ataagtatgg
     2041 acattttcca tgggcatgcc cctcctttgg gcaaatacgc agttatggat acgggcggga
     2101 gcattactcc gcgtccggcg tttcgccgga ggccggctct atggctgcga acagactgcg
     2161 cagccggata ttcaccgcgc ccagattcgc gatctcatcg attttgatct gtgtgtacag
     2221 cttgccttcc cgctgcaaaa tttctcttgt ttcgtcggtg gtgatggcgt ccacgccgca
     2281 gccgaagctc accagctgga cgaggttcat atccggctgg tccgcaatgt atttcgccgc
     2341 cgcgtacagg cgggaatgat aggtccactg gttgaggatg ctggtgggga atttttccac
     2401 cagatgtgag atgctgtcct ctgtgatcac cgccgctcca tagctgcaga tcagcttgtc
     2461 gatgccgtgg ttgatctcct catccgcatg gtacggacgg cccgccagaa cgatgatctg
     2521 ccggccctcg gcccgcgcct gggcgatgat ctccccgccc cggacacgca cgcgggacat
     2581 atgctccgca tattcggcat aggccgcgtc cgcagcgccc ttcacctcgc ccaggctgat
     2641 gcccgcgaag tattttgcaa ggatggccgt catcttcctg ggaaaatccc ttcggcggtg
     2701 aatgccgata taatcataaa taaaacggat attctccagc tcgtggcagt tggccgcaat
     2761 gacctccggg taataggcca ccacagggca gttgtagtgg ttgtcaccca ggccctcatc
     2821 cacattatac gtcaggcagg ggtaaaaaac ggcgttcaca gccatccggc tgagccggtg
     2881 gatatggccg tgcagcagct ttgccgggta gcatacagtg tcggaaggaa tggtactctg
     2941 gccctccagg taaagctccc ggctggaggg accgctggtc accaccgaaa agcccagctt
     3001 cgtgaagaaa gcataccaga aaggcagcag ctcgtacatc cccagcccca gggggatgcc
     3061 aatagtgccg cggatgcccg gctggggctt gtattcggcc agaaggcgct gcttgtattc
     3121 gtacagattc agtgggtttt ccgcgccccg gtgggacacc ggacggtcgc accggttgcc
     3181 gctgatgtat tttttgccgc ccggaaaaat attgaccgta agctggcagt tgtttccgca
     3241 caggccgcag ttgacgctgc gcaccttctg ggtgaagccc tccagctcct ccagcgtaag
     3301 cagagacgat tggctctttc cctttgcctt gccgtacagt gccgcgccga acgcgcccat
     3361 caggcccgcg atatccggcc ggatgacctc cacgcccatt tccatctcga acgcacgcag
     3421 tacagcctca ttgtagaacg taccgccctg caccacgatc ttgcggccca actcctccgg
     3481 gccggaggca cggatgacct tgtacagcgc gtttttcacc acgctgatgg caagcccggc
     3541 cgaaatgttc tcgatggacg cgccgtcctt ctgggcctgc ttgacgctgg aattcatgaa
     3601 caccgtgcag cggctgccca ggtccacggg cttatccgca aacagagcca gcttcgcaaa
     3661 ctccggcacg ccgtagccca gcgcgcccgc aaacgtctgc aaaaagctgc cgcagccgga
     3721 gctgcatgcc tcgttcaaaa agatgttgct gatggctccg tcctcgatct tgaagcactt
     3781 catatcctgc ccgccgatgt cgatgacgaa atccacgtcc ggcataaagt gcttggccgc
     3841 cgtaaagtgg gccactgtct ccaccacgcc gaaatccacg ccgaaggcgc ttttcacaat
     3901 gtcctcgccg tagcccgtgg ctgtgacgct ggccacccgc aggcccggac gcgccttgta
     3961 aatgtcgatg agcatctgcc ggatgatggg gatgggattg ccgctgtttg gctgatagct
     4021 ggtgaaaagc aaattgtcgt ctccgtcgat gacggcggct tttaccgtgg tgctgcccgc
     4081 gtcgatgccg atatgcacag ggccggtgct gtccgcaaag ggacggcgcg gcacgctcgc
     4141 ctttgcatgc cgtgcgcgga acgcctcgta ttccgcttcg tcccggaaca gcggcgggtt
     4201 gaacgcatag gtggccgtgg cgctgtatgc gtgcagcttt tccaccacac ccgccaggtc
     4261 cagttccttg tcggaataca gcgccgcgcc cagcgccaca aaataaaggc tgttttccgg
     4321 gcaggtcccc gtcacgccca gcgtcttgtc gaagctcttt ttcagctggg gcaaaaatgt
     4381 gagcggcccg cccagataca gcacgttgcc gccgatcttc cggccctgcg caaggcccgc
     4441 gatggtctgg ttcaccacgg catagaaaat gctggccgcc acatccccgg cctttgcgcc
     4501 ctggttgatg aggggctgga tatcgctttt ggcaaacacg ccgcagcggc tggcaatggt
     4561 atatgttttt tccgcatgcc gcgcggcttc gttcatttcg tcgggcgtca tcttcagcag
     4621 cgttgccatc tggtcaatga acgcgccggt gccgcccgcg cagcttccgt tcatgcgcac
     4681 ctcggtaccg cccgtcaaaa acaggatctt cgcatcttcg ccgcccagct cgatcaccac
     4741 gtccgcatcc ggcgcgtaac gctttgccgc cacccgtgtg gcaaacacct cctgtacgaa
     4801 gggcacgccg caggcgtcgg ccaggcccat gccggccgaa ccggagatgg aaagctgcgc
     4861 gggcgcgccg tgcagcacct cagtgtgcat gcgctgcacc agctccgccg ctttttccac
     4921 gatgtgggaa aaatgacgct cataggtttt gtatgtaata ttcagcgcgt cgtccagcac
     4981 cacgcatttg atggtggtgg acccgatgtc aagcccgatc ttcaaactgt tctccctttc
     5041 cccttaagcg gcgcgccggt aagacaggac ctcctatctt aaccttatct ttaccttata
     5101 tcatagtatt ttaccgcttt ttgtcgaaaa atcaacggac agatgtgaat atttgtcaaa
     5161 ttttttacgg aaaattcgtc cttatttaca aaatactggc gttatgccca aaaacacagc
     5221 cttttcttat caaaacaggc ccacagcgcg gagccgcagg cctgtaaaac caattattct
     5281 gtgcgcagcg cctctatggg gctcaggcgc gccgccttgc gcgcggggta aatgccgaag
     5341 aaaatgccga tggcgatgga aaacagcgcg atataaacta cagtggaggg tgccacatag
     5401 ccctccatgc ccatcaggct gctgatgccg tagccgcccc acaggcccag cacaataccg
     5461 atgacgcctc cgatgagcgt aagcgtaccc gcttcaatga ggaactggaa cgtgatgctg
     5521 cccgtctttg cccccagcgc cttgcggatg ccgatctccc gcgtgcgctc ggtgacgctg
     5581 acgagcatga tgttcataac gccgatgccc cccaccagca ggctgatgcc cgcgatggcg
     5641 gcgatgatgc ccgtaaacat ccctgtcacg ctgctgatct gcgcggtgta ctggcttacg
     5701 tccataatgc cgtagcagtc ccgcccggcg ttgccgtgtc ggctttccac cagacgcagc
     5761 acctgctgtg ccagcggtac actgttcgca gtgtcctgcg ccagcagcgc gatctggaaa
     5821 tacggctggc ccacatcgtc ggaaagagtg gacaggctgg acagcggcgc cgtcatctgc
     5881 gcagtgatct tgccgttgta gctgtatccg ctctggcttt cggttatgcc gatgatcgtg
     5941 aaatccgaag tgcgtccgcc caccgtgagc tgcaccgtca tgcccaatac gtcgtcggtt
     6001 ccaaacagcg cctttgctcc cgcggaatcc acggtcacaa cacggcgcgc ggcatcataa
     6061 tccgacgcgt cccaaaagcg ccccgcctgc agcgtagccg gaaacaccag aaacatgctg
     6121 ccgttgcccg ccgaaaggct ggcgcctatt tctccctttg ggcnnnnnnn nnngggccgc
     6181 gcacacttcc gctggcgccg ccggccatcg tcacgcccgc aacaccttcc atacggccca
     6241 cggcctccac gtccccgtcg gtaaaataat cgttcacagt ggtatcctcg ccggtcacac
     6301 tcagatacac cgaaccggaa gacagcatgc ccagttcgct gtccatcatc cgctgcccgc
     6361 cgcctccgat gcacagcacc ataatgacag ccccaatgcc gatgatgatg cccagcatcg
     6421 tgagaaatga gcgcattttg ctgcccatga tgttgcggta cgccattttg gcgtattcat
     6481 agatctgacg catccggacg cccccttagc ccatcatcac agcgcccata cccgcgtccg
     6541 cgggcatctg cgccgcggcg gcggggtcgc tgctgacagc catgccctcc gtcacatcct
     6601 gcgggttgag gatgacctca tcccccgcgg aaatgccgga ggcgatctcc acctgcgtat
     6661 cggaagaaag gcctgtcttc acatcccggc gcacagccgc gccgttttcc accacatagc
     6721 agaactggcc gctcacatcc gtattgacgg cgctgatggg caggctcagc actcctttcg
     6781 cctcccctgt ggcaatgacc acgttggcct ccaggcccag tttgatatcg ccgtccggcg
     6841 cgtccagctg cactttggcg tgcacatagg ccgtggccgt gcctgtggcg gaggccccgg
     6901 ccgtggccat cgcgtcgatg gacgccaccg ttccggtgta tgtcctgccc agcgtggtga
     6961 caacggcgct ctggcccggc tttacctttt ccagatcgta gcgggagagc gccacgtcca
     7021 catatacatc gctcaggctc tggatcgtgc acagcggacc ataagccgcc gcgctggagc
     7081 ccggcaccgc actgagggag gtgacgacgc cgctgatggg tgcggtcacg ccgtccctgc
     7141 cgcgggcaag gtcctccttg gcgctttgca cggagaggga tgatggcacc tgtgccagct
     7201 ccaactggcg cttggcgctg tcggacagga cggcctgctt ttccgcgtcg taggttgcct
     7261 tggccgcgct cagcgcctgc tgctggttcg cgaggtccgc ggcggcggcg tccaaagccg
     7321 ctttaacaga caacgcctcc tcgctctgct tatccgcgag ggcctcgtac tgcgcggtca
     7381 ggctgttcac cgcggctgaa gccttgtctt tctggacggc aagattgttc aggttcgcag
     7441 ccgcgtcatt gaagcgtgcc tgtgcgctgt cgctggcggt gagcgcgtcg cttttctgca
     7501 gctggccgct ctcataagcg gcagaagcct gccggtaggc gcgctccagc ggctctgtat
     7561 caaaggtggc aagcaggtcg cccgcgttca cgatctgccc tgcttccacc ttcacctctg
     7621 caagaggcgc ggtgacgggc gacggcacca ctacggtctg ccccgaggtg agcgtacccg
     7681 tggtggacag gctttggctg atgtctccgg ctgccgccgt ctgtgtttgc acataggtcg
     7741 gcagcgggcc gcgcagcgcg ctcagaacgg cgttgcccgc cagggccagc accgcaatgc
     7801 ctgccagtga aagcagcacg atttttttga tgctgcgctt tttcttcggc cgtttgggcg
     7861 gctgcaaaag cacctcgccg gcctgcgccg ggtcctgcac gttctgcgcc atcaattcct
     7921 ttgtttccat tttagaaaac ctcccgttcc ccgtcgtcta tttttccgtc tgtgatacgc
     7981 acgatgcgct gagccttgcg ggccagccct tcgtcgtgag tgatcagtac aatggtgttg
     8041 ccctcggcgt gcagagcacg gatgatctcg aacaccttgg cgcccgctct ggtgtccagg
     8101 ttgcccgtcg gttcatccgc gaggatgagc ggcggatgtg ccgcgatggc gcgagccacg
     8161 gccacacgct gctgctggcc gccggacatc tggtttggac ggtggtccat acgggacgca
     8221 aggcccacac gctccagggc ctccaacgcc agcagacgcc gttttccggc gggcaggccc
     8281 cggtaaatga gcggcagctc cacgttttcc agcgcgttca ggttcttgat caggttgaag
     8341 ctctggaaaa taaaaccgat ctgttcattg cggatggctg aaagcgcatt atcggaaagc
     8401 gcgcttacgt cctgcccgtt gagaaaatat gtaccggacg tcggcgtgtc cagacagccg
     8461 atcatattca tcagcgtgga tttcccgctg cctgaatggc ctacgatggc cacaaactcc
     8521 cccttgtaga ccgcgaggct cacgccgtcc agcgcgcgca cctcgttttc tccgggattg
     8581 tagatcttgt acatattccg gatatcgatg atggcctgtt ccaagcccat ccctccctgt
     8641 gcccgctgcg caggcatttt ttccatctga catttatgca acgatatggt ccggccggct
     8701 tcttcatttt ctgcgcgcaa taacgaaaac cgttccctta ttttcagcaa aaggccaaaa
     8761 atgcagattc ccacacaaaa tatccggctg tgcatgtact atattttccc cgcgccgcat
     8821 atccaaaagc gaggaggtgt ccccttatgg aactgatgga cggcgtaatg gaatatatcc
     8881 ggcccgagct gctggtgctg gtggcggtgc tttacatcgt cggcaaagca ctcaaggcct
     8941 cgcaggacgt aaaagacaag cacatcccgc tcattctggg cggcgcgggc gtgttcctgg
     9001 ccatgctgtg ggtggtggca accggcagct tcgacggcgt acagagcatt gcgctgggcc
     9061 tgtttaccgg tatcgtgcag ggcgtactgg tggccggctg cagcgtatac gccaatcagc
     9121 tcgtcaaaca ggcgggccgg gcggattgac ccgaatgcaa aagagcatcc ccggcgcacg
     9181 gataccccgc gccggggatg ctctttgttt tctgatattc tcagggaacg tacacgttta
     9241 catacttcac accgtttgcc acggattcgg cataggtctc gtagaacagg tccacctgca
     9301 tgcggttgga ctgcacaaac gcacccgtat ctgtcgcaat ggcccagcca tatacgaatt
     9361 tgccgtcagc ggaggtgata tacacctttg taccgtaggg aatgagcgtc gggtcgcacg
     9421 caaaagtacc gtaatacagg cccaggccgc ttgcgccgcg tccgcgggcg gaataatagc
     9481 ctgtcgcctt catggaatac attttgctgt agctgctggg cacgttgttt tccaccgtga
     9541 tgccctcagg ggcctgcagc gggctgacgg cgtcggaatt gtatttccgg tagatctccg
     9601 gtatcatctc ggtgacgacc tgggtatcca ccacctcggt gctggaatat ccgccgtcca
     9661 catacttctg gcggtaggtc acgtccacaa ggccgtcctg ccccgcgtgc accagcacct
     9721 cgtgggaata tttccccgtg ctttcggcgg tttcgtccag atactccgtc tcatagggaa
     9781 cggtctcccg ggcggtattc tcagtatatg ttacacggtg tacgcgcaca cggcccatgc
     9841 cctcggtcac cgggctgtcc agactgggag tggtgtaatc gtccggcccc agcgtcacgc
     9901 ccgcgcgggc cagcacgtcg gccacagtgc cgtcgctgat ctccgcctcg taatattctc
     9961 catccgccgc tacagagacc ggaaaagccc gctgcagaga aagaagcagc gtgccgtcct
    10021 ccacctcgtg cagacgggtg ctgtcgttgc tctccagttc catacccgcc aggtccagca
    10081 ggacctcggg ctccgtcacc gcagccatca ctgtgcgctg cgcaccggcg gaatccgcca
    10141 cagtaaccgt attcaccgta gcggccaatg ccgtgcacac cagccccagg ctgcccgccg
    10201 tcgccgccac cgcaacgatg cggggcccgg cggcggccag ccagcgccgg atatgctcct
    10261 gtcgctttgt catgcaaaaa ctcctttggt tctccgtcca cacaccgggc cgcacagcca
    10321 aaacaaaacg gacggcccgc accagaccgc ccgtgtatca acaaaaaaga acagcgccgc
    10381 gcgcgttttt gaaaacaaaa acaacggatg catgcggcag gggcaggcct tggtacctgt
    10441 gcattatagc aaaagaaagc cgccgcgtca aattttcggc ttcaaaccgc catttttcta
    10501 tacaattggt aatattttac gcagattttt tcttttttgc catcctgtcc agacgctccg
    10561 cgcacgcttc gccacggcgg cggacattgc atgtttcccc ctgtgcaaac cctgattttt
    10621 atggtataat aacccccaaa gctgccgttt acacggctgc gcatgtgcac tgcacacgct
    10681 cctgtgctgg cgcacagagc tttgggaaca ttgaaccatg caaaaccgca gcaggtgcgg
    10741 ttttgttgtg gccgtttcac ggcgtcatgg tataatatgc ccgcttagcg aatgcgagcc
    10801 gcaggcgaag caaaagcagg gcggcataga aacatggtat acgaagcatt tagagacccg
    10861 gcggcagcac cgcccggcgc ctatcattct gattcgacgg aggaacgccc cttggcacag
    10921 aaaaacatcc gcaatttctg catcatcgcc catatcgacc acggcaagtc caccctggcc
    10981 gaccgcatcc tggaatgtac ccaggccgtg gacgagcgcc agatggaggc ccagcttctg
    11041 gataacatgg agctggagcg cgagcgcggc atcaccatca aggcgcgcgc cgttaccctg
    11101 cactatacgg caaaggacgg gcaggattac gagttcaacc tcatcgacac gccgggccat
    11161 gtggacttca actacgaggt gagccgaagt ctggccgcct gcgagggcgc cgtgctggtg
    11221 gtggacgcca gccagggcgt ggaagcccag acgctggcca acacctacct tgcgctggag
    11281 cacgatctgg agctggtgcc catcctgaac aagatcgacc tgcccagcgc cgagccggac
    11341 cgcgtggccc acgaggtgga ggacgtgatc ggtctgccgt gcatggacgc accccgcgtc
    11401 tccgccaaaa tgggcgtggg catccaggat gttctggagc gtgtcatcac cgatatcccc
    11461 gcgccgaaag gcgatgcaga tgcgccgctg aaagcgctga tcttcgacgc ggtgtacgac
    11521 ccctacaagg gcgtcatcgt ctatctgcgc gtgttcgacg gccgcctttc cgtgggcgag
    11581 cccgtgaagc tgatgcagac gggcgctcaa ttcacgcccg tggaggtcgg ccatatgggc
    11641 gctacggcgc tggctccctg cggcacgctg gaggcgggtg aggtaggtta tttcaccgcg
    11701 tccatcaaaa cgctggagga cacccgcgta ggcgacacca tcacgggtgt gtccgctccc
    11761 acggctgagg cgctgcccgg ctaccgcaag gtgacgccga tggtgttctg cggcatttat
    11821 cccgccgacg gcgcggatta tcccgacctg aaggacgcgc tggaaaagct tcgcctgaac
    11881 gatgcgtccc tgagctttga cgcagagacc agcgtggcgc tgggctttgg cttccggtgc
    11941 ggctttcttg gccttctgca tctggagatc atcatcgagc gcctggagcg cgagttcgac
    12001 ctggacctta tcaccaccgc ccccagcgtg gaataccgca tcacgctgac agacggtgcc
    12061 gtaaaactca tcgacaaccc caccaattac cccgatccgg cgcaaatcgc caaggccgag
    12121 gagccgttcg tggatgcgca tatctatacc cctaccgatt atgtgggcag cctgatggag
    12181 ctgtgccaga accgccgcgg catcatgcgg gacatgaagt acatggacgc cacccgtgta
    12241 gatctgcatt atgagcttcc gctgggcgag atcgtatacg acttcttcga cgctatcaaa
    12301 agccgcagcc gcggctacgc cagctatgac tatgagatga agggctttgt ggaatcgaag
    12361 ctggtgaagc tggacatcct gctcaacggc gacatggtgg acgcgctttc gatgatcgtc
    12421 cacgcggata ccgcttatgc caagggccgc cgtctggcgg aaaagctgaa ggaaaacatt
    12481 ccccgccaga tgttcgagat ccccatccag gcggccatcg gaggcaagat cattgcccgc
    12541 gaaaccgtga aggccatgcg caaggacgtt ctggccaagt gctacggcgg cgacatcacc
    12601 cgtaagaaaa aacttctgga aaagcagaag gaaggcaaaa agaagatgcg ccagctgggt
    12661 acggtgcagg tgcccagcga ggcgttcacc gccgtgctca agctggacga atgagcgcag
    12721 cgcccggccg ccgaagcttt ctgcctttgg tttgaaggtc tggagaaaat attatgaagc
    12781 tgaaatgtgt gaatattgcg acgccaaacc cctccgcaat gagccgattt tactcgctgg
    12841 tactgggcca gccctgccgg cggcgcgctc caaaccgctt cgaaattccc gccggcggcg
    12901 cttttgtcgt tatcacgcac tccgacacaa aaacacctgt caaccccgat tgctgcggac
    12961 tggagtttgc ggtcgacgac gtagacgcgg aatacgaacg tctgctttct gcgggtgttt
    13021 ctgtggaacg ccctcccgtc acttacccgt gggggtaccg cgccatcgga tttcgcgatc
    13081 cggacggcaa taacatcgat tttacggcgt atgttggtaa accggaataa ggaacacgac
    13141 ggcagtatgc aaaagcggac ggctgataaa acagccgtcc gcttttattc ttgtcgcccg
    13201 tacaccttac agctccggtt cggccgaagc cgccgcgccg ctctgcgcgt ccgaagacgc
    13261 gccctcgccg ccgatgacgc ccgtggggct gacgccgccg aacagcagcg tgccttccct
    13321 gtccttaccc acgatagcca cgtaactctt gcccacattg acctgcagcg cagcgccgtt
    13381 ctcgtccttc aggcgcagcg ggtcctccgg attgcctttt tcccatgtca ccgcgcggaa
    13441 cttgccgccg tagcagtaat agcccgtgcc gctgctcata gcgaagtccg tcacaagatt
    13501 gttggggtcg tcgggatttt tc
//
LOCUS       DAWBSB010000029        13499 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1122_length_13499_cov_3.562630, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000029 DAWBSB010000000
VERSION     DAWBSB010000029.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 13499)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13499)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13499
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1122_length_13499_cov_3.562630"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    261..270
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3022..3031
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3042 a         3540 c         4294 g         2603 t
ORIGIN      
        1 ggcctgcacc atgtggtgta ggaaatcgtg gacaacgcca tcgacgaggc gctcgccggc
       61 ttctgcgacc atatcgaggt caatatccgg gagggcaata tcatccgcgt aacggacaac
      121 ggccgcggca tccctgtgga tatccagccg aaattgggca tcccggctgt taccgtggta
      181 ttcaccatcc tgcacgcggg cggcaaattt ggcggtgaca attccgggta caaggtcgcc
      241 ggcggtctgc acggcgtggg nnnnnnnnnn aagcgcggtg tggctgacgc cgacctgcag
      301 aatctgggcc ccacggacga accggacgga acgacggtca cgttcaagcc ggaccccgag
      361 atgttcgagg tgctggacta cgattatgac actctgaaca cacgcctgcg cgaagaggcg
      421 ttcttgaatg ccggcgtgcg catcacgctg acggacgagc gcgccgggca ggaaaagcag
      481 gagagcatgt gctacgaggg cggcatccgc agctttgtgg accacatcca ccaaaagcgg
      541 cagcttgagg tgctgcaccc ggacgtaata tatctgaagg ggcagcttaa cgactccatc
      601 gccgaagtgg cgcttcagta taacaacagc tacaacgagc tgatcctttc ctttgcaaac
      661 aatgtaaaca ctccggacgg cggtacccat gaggagggct ttaaatccgc gctcacccgt
      721 gtgttcaacg atttcggccg tgaaaagggt tatatcaagg agaaggacga caacctttcc
      781 ggctcggacg tgcgcgaagg cctgacagcc gtcatcagcg tgaagctgac ggaggcgcag
      841 ttcgaaggac agacaaaggc gaagctgggc aataccgaga taaggggcct tgtctccggc
      901 atcgtgtacg acaagctgaa ggaatttttt gaagaaaatc cggcggtggc aaaggccatt
      961 tatgaaaaag ccatgcaggc cgcccgtgcc cgcgaggccg ccaaaaaagc gcgcgagctg
     1021 gtgcgccgca agtctgcgct ggaaaacagc cgtatgcccg gcaagctggc ggactgccgt
     1081 gagaaggacc ccacgaaaac agaaatctac atcgtggagg gcgattccgc cggcggcagc
     1141 gccaagatgg ggcgcgattc caacatccag gccattttgc cgctgtgggg caagatgctc
     1201 aacgtggaaa aggcgcgcat cgacaaaatt tacggcaacg acaagcttac tcccgttgtg
     1261 acggcgctgg gctgcggcct gggcgaggag ttcgacgtct ccaagctgcg ctaccacaag
     1321 gtattcatca tggccgatgc cgacgtggac ggcagccata tctgcacgct gctgctcacg
     1381 ttcttcttcc ggtatatgcg tccgctcatt gaaggaggtt atgtgtatat cgcacagccg
     1441 ccgctgttca agctgcagaa gggcaagcag gtacgctacg cgtacaacga gcctgaaatg
     1501 agccagatct ccgccgagat gggccagggc accaaggtga gccgttacaa aggccttggc
     1561 gaaatgaatc ccgagcagct ttgggagacc acgatggacc cggagcaccg tgtgatcgtg
     1621 cagatcacca tcgaggacgc gatggccgcc gacgaggcgt tcacaatcct gatgggcgac
     1681 aaggtggagc cgcgcaaaga gttcattgaa aggaacgccc agtacgcgaa tctggatgtc
     1741 tgacggcgtg gcccgtcgga caatgccgta taaaaagcgc acggctgcag ggcagcacgg
     1801 cgcacaagtg cctttgaaag ataggaagat aacatgattg ataacacgat aaccgaaggg
     1861 acgaggctca tcgtccggga catcaaggag gaaatggagt ccgccttcat cgactattcg
     1921 atgtccgtca tcacggcccg cgcgctgcct gacgtgcgcg acggcctgaa gcccgtgcac
     1981 cggcgtattc tgtacacgat gcacgagcgc ggcaacgacc cgtctcatcc caccaaaaag
     2041 tccgccgata cggtcggcgc ggtgctgggc tcgtaccatc cccacggcga cgcatccgta
     2101 tatgacgcga tggtgcgtct ggcgcaggat ttctccatgc ggtacccgct tgtggaggga
     2161 cagggaaact tcggttctgt ggacggcgac ccccctgccg cctaccgtta cactgaggcc
     2221 cgcatgagca agatgagcgt ggacctcatg cgcgatatcg aaaaggatac cgtggatttc
     2281 gtgccgaact tcgacgagtc caagcaggaa cccagcgtgc tgccctgccg cgtgccgaac
     2341 ctgctcatca acggctccag cggcattgcc gtgggtatgg ccaccaatat tccgccccac
     2401 aacatgcggg aggtcatcga cggcatcgtc gcgctgatcc atgacccgga catcgacctg
     2461 gccggcctga tggagcacat caaggggccg gacttcccca ccggcggcgt cattatgggc
     2521 cgcagcggca tccgtgccgc atatgccacg ggccgcggca aaatcacgct gcgcggccgt
     2581 gctgccatcg aagaaaaatc caacgggcgt tttcagatca tcatcaccga gatcccctat
     2641 atggtgaaca aagcgcgcat gatcgaaaac atcgcggacc ttgtgaaaga aaagcggatc
     2701 gacggcattt ccgacctgaa cgacgaatcc aaccgcgaag gcatgcgcgt ggtggtggaa
     2761 ctcaagcgcg aggcgaaccc ccaggtggtg ctcaaccaat tgtaccgctt tactcaattg
     2821 caggacaccg ttggcgttat catgctggcg ctggacaagg gcgtgcccaa ggtaatgacg
     2881 ctcaaagaga tgcttcagaa atatctggcg tttcaggatg aggtcatccg caggcgcacc
     2941 cagttcgacc tgaagaaggc cgaggagcgc gctcatattc tggagggcct gcgccgcgcc
     3001 gtggacatcg tggatgagat cnnnnnnnnn naaaaattcg gttttgacga cccgcaggca
     3061 acagccatcg tcaatttcca gctgggccag ttggcgggcc ttgagatttt gaaaatagag
     3121 aacgaattgg gcgacctgca tacgaagatc gacgactggc gcggcattct ggccgacgat
     3181 gcaaaggtgc tgcaggtggt ggaggatgag ctgaacgcca tgcgtgaaaa gtacggcgat
     3241 gaccgccgta ccgagattgc ccatgtctcc ggcgaagtgg atattgagga tctcatcccc
     3301 gaggaggaaa gcgtgttcac gttgacgcac gcaggctaca tcaagcggca gccgtcggat
     3361 acgtatcagg cgcagcgccg cggcggccgc ggcatcacag ccttgtcccg caaggatgaa
     3421 gattttgtgg aagagctgtt ccttgcctct acgcatgact atatcctgtt tgtaacggat
     3481 atgggccgcg tgtaccggct gaaaggatac caggtgtacg agggcagccg taccagccgc
     3541 ggcgtgaaca ttgtgaacct gctgcctctg caggacggcg aacaggtgac aagcatgctg
     3601 cgcgtgcccg gcggcgacaa cgccgaaggc tatttgacga tggtcacaaa agcgggcgtc
     3661 atcaagcgca cggctctggc aaattacagc aacatccgca aaaacggact tatcgccatc
     3721 aacctgaacg agggagactc ccttgcgtgg actcgtatca ccagcggcga agacgagctg
     3781 atcgttgcga cgcggaacgg catggccatc cgcatcagtg agaacgacgc ccgtccgctg
     3841 ggccgcacgg ccacaggcgt ccgcgccatc cgcctgaaag agggcgacag cgttgtgggc
     3901 gtgggtgtcg tacgtgaggg cgcaacggtg ctcaccgtta cggaagaagg caaggggcgg
     3961 cgcacggacg tgcgggatta ccgaacccag taccgcggcg gcctgggaat ccggaactac
     4021 ggcagcaaag gccacgtcgc ggggcttaag gtcatcgacg acaccgacga tatcattctc
     4081 atcagcctgg agggcatcat catccgcatg catgtggagg atatcaacgt gcagagccgc
     4141 tatggttcgg gtgtgcgcgt gatgcgcctg ggcgagggcg accgcgtggt gacggttgcc
     4201 cgcaccgagc attccgacga ggaggagacc gcaaagcccg aggacgacgg cgccgaggag
     4261 gagctttccg ccgaagagct ggcggctctg gaggctgagg aagcgcagaa tgccgccggc
     4321 gaagaggacg cggcgccgga ggacggcgag taagctttcc gctccggcag aaaagagccc
     4381 ggctgaaaag cagccgcagc aaggaaccgt cccccggccg gggaaaaaat aaggatggaa
     4441 gggttgcttt ttgaaacagg gaaaacagat ctcagcggtg ctgttcggag ttttggccgt
     4501 atttcagatg ttcattgtgc ggtacatgat gaacaatgcc gcgctcacgg gcgtgcccat
     4561 tatggttttg gcggagatcg cggccgcacc cgtttttgcg gccgcgtgcg tgtggctgtt
     4621 cagccgggac ggcgtgcttt ctcccgtggg caaaaaggtg cttgcgggca gcgcgctttt
     4681 ctatctgctg tttattgcgg tgtactatga tttggaggtc aatctgatcc tttccggact
     4741 gtccaccctc gggcaggaat tggcgaatgg tttgacgccg ggctatctcg gcatggcggc
     4801 gaaggtggtg ctgctggtgc tggccgtcgg gtttgccgta acggcgccga aaaagcccgg
     4861 cgagccgtcg gagctgaagg aagtgctcca ggaggcggcc gaggcgatga gcctggacaa
     4921 ggaaaccatg gaaaaagaag tggaacaggc ggtttccgcc tttgaagaaa acatcggcgc
     4981 cgaagaactg gacggcggcg agaaacagta aaaggagaag cgtcctatgg atatgaaaaa
     5041 ctggaatgta gacaaaacac agatcgccgc ccagcaggag gcactgaaaa aaagcatgaa
     5101 ttttgcttat gtgggccacg gcccaaaagc cacgggcttt atggcaaagc agtttaaacg
     5161 taagccgggc ctgtatgcgc gctgtgtgca gtgcggctat tacatgcctc tggatgccaa
     5221 gggcgaggag acctgccttt gcggaaacct gcgccgcgac gagcacagct ttaaaagcga
     5281 tctgcccgcc actgaagtgg aaatcttcaa gggcacgaag cggtaagagg gcgcaaaaaa
     5341 gcgacatacg gccgcccgtg cgcggagagg agcgcttctc tcccgcccgg acggctttcc
     5401 ctgttttatt cggatcaaag gaggccgtat cgtgacagcg gcggagtacc ttgcctcgga
     5461 gcctgtgggc aatgcagatc ttttggagat gctgcgccgg ggcacggcgg atgttttata
     5521 ctgcacacag cacgcgctgg ccatgcatga gcgtgtcagc ggcgcggcgg tgctttgcgc
     5581 cgaaacagag cgggaggcgc gcgcggcgct ggaacatctg ccccgcaccg ggctttatgt
     5641 ggcccacggt gcgttgtgtg cccgccttct tcgggaggaa acaggctgta caggcggaca
     5701 aaagtgccgt caggcacggt atttgcggcc tgtgccgccc gcgcccgtgc cgggcgtgga
     5761 actgcgcatt ctggatgaaa gccatacaga ccttgtgttt gaacactatc acatggcgcc
     5821 gggccgggat tatgtagcgg aacgccttgc cgcgggcgca atgacgggcg catttttgga
     5881 tggcgtgctg gcgggcttca ttggcatcca cgaggaaggc agcatcggta tgctggaggt
     5941 cctgccggct tatcggcggc gcggccttgg ctacgccctg gaggcgggag ccatccgccg
     6001 cgcactgaag gagggcgcta tcccctattg tcaagtcatt gagggcaata cggcttctat
     6061 ggagctgcag aaaaagctgg gtctggaatt ttcagatggg tttgtgtatt ggctgttcta
     6121 aaatatttga gctgcctgaa gatatgttct atcttgagaa ccattcacat ttgtgctaca
     6181 attaaacgaa tttgaaaaga aaggggcatg cgggctttgg tgcagcagaa tgggaccatc
     6241 gcggccattg ctacgccgcc gggcgtgggg ggcatcgccg tcgtgcgtat ttccggcagc
     6301 ggggcttaca gcgtggccga agcggtattc cggcccaaaa atccgaataa aagcctgcgt
     6361 gccgcgtcgg gttatacagc gctgttcggc cattttgtgg aggacggggc gctgtgcgac
     6421 gaggtggtag cgctttgctt ccgcgcgcct cacagctata cgggcgagga tgtggtggag
     6481 ctttcctgcc acggcggcag cgccgtgagc gcgcgcctgc tgcgcgcctg ctgtgcggct
     6541 ggcgcagctc cggccgggcc gggcgaattt acaaaacgcg cgtttttgtc cgggcgtatc
     6601 tcgctcacac aggcggaagc tgtaatggat ctgatcaacg ctacctcccg gcaggccgcc
     6661 gctgccgccg ctgccgccat ggaaggagcg ctgtacgcaa agataaaatc tgtgcaggaa
     6721 aaccttgtgt cgctcgccgg acatatcgct gcctatacgg attatccgga ggaagatgta
     6781 ccggagcttt cgatgggggc gctggaagct tccctcgggt gcgcgaaggc agcgctggat
     6841 aagctgattg cgggctatga tgcgggcgcg gtgctgcgcc gcggtgtgga cacagccatc
     6901 atcggcagcc caaacgtggg caaaagcacg ctgctgaacc tgctctccgg ttttgagcgt
     6961 gccatcgtta cgcccgtggc gggtaccaca cgggacgtgg tggagcagga gatccagctg
     7021 gccggggttc gtctgcttct ggcggatacg gcgggtatcc gggacaccga cgatctggtg
     7081 gaggcggaag gcatccgccg cagctaccgc cacttggaac gcgccggcct ggtccttgca
     7141 gtgttcgacg gctccgcgcc tctctccccc gccgatcttt cgctggcgga aaagtgcgcg
     7201 ggccggccgg ccgtggcgat cctcaataaa acggacctgc cgcagcaggc ggatgaagaa
     7261 agtttgcggc cttatttcag cacggtggtg aacatcagcg cgaaggacgc ggcgtttctt
     7321 ccggcggtgg agcaggcggt cacagacgcg cttcatttgg cggaagcgga ccccgacgca
     7381 gggcttctgg caaatgagcg ccagctcgct gccgcccgca cagcacaggc tgcgcttgcg
     7441 gacgcgctgg aggctgtaaa cggcggatac acgctggacg cagcgggcgt atgcgtggat
     7501 gacgctctgc atgcgctgta cgaattgact ggggaaaatg ccgggaatga tgtgatcgac
     7561 gaagtatttt ccaggttttg tgtgggcaaa taaagtacaa taattaaaat tatattataa
     7621 tattacaaat tgtattatat tttggtcgtt tctttacaag aaaacaaagc gcacggaaag
     7681 acgacctttc ggaaaggaat ggaaaaatgg attctgctgc gttggcacag gcattgtggg
     7741 cggatatcgg cgcgcagcgg tgggatgcgc tggccgcata tttcctgcct ggtgcgctga
     7801 ttttctggca taacactgac gagtgcttca ccgtagagga atttgtgcgt gccaaccgtg
     7861 aatatcccgg gaactggcgg atagagatag aacgtgtcca tgtactggcg gatgcggcgg
     7921 caacagccgt gcgggtatac ggtccggatg gaacttcttt ccacgccgcg tcatttttta
     7981 cgttccggca cggaaaaatc gtccgcctgg atgaatattg gggcgatgac ggccccgcgc
     8041 cggagtggcg gcgcgcgctg ggcatcggca ggcccatcca aagggaggac cgttatggcg
     8101 gcgaatgatt atctgatgcg tcagatcgag gatatggccc gttttttggg gaaagtgctg
     8161 ttcgtcaaaa ccgaagagac gattccgttg ttcgacgaac aggacaatgt actggagagc
     8221 ggtttgcttt ataagcgcct gtgtgccatg ctggaggagg gcgatgcaaa cagcgcggaa
     8281 aatctgctgt ttgacgaagt ggaagctcat cccgacccgg catatttgca ggtggctgtg
     8341 cagttctatg ccgatttgca gcattggacg gacgcggcgc tggaagcggc tgatttttcc
     8401 cgtcaggagg ttttggacgg ccttgcagcc gtgaaggcgc tgtatgagaa aagggccggc
     8461 gggcaaaaag cgaaagaata aaacaggaga gctgcagtat gacggaattt cggtatgaca
     8521 cacagctcct gatcgagggc gaaaacctgg atgaggacac ggtgcacgac catttcacac
     8581 ggcactttaa aggcgactgc ctgctggccg tgggggatga aaatctcatc aaaatacatt
     8641 ttcacacaaa cgaaccctgg caggtactgg aatattgtgc gggactgggg gaaattttcg
     8701 atatcgtggt ggaggatatg gaccggcagg cgcgcggctt aaagggctga gcggcaggcc
     8761 ggacaaagaa acggggcaaa cggacattgg atattttagg cggttatgaa gttgtggtca
     8821 tcggtgcagg gcacgcggga atagaagccg cctgcgccgc ggcgaagctg ggtgcaaaga
     8881 cggcgctgtt cacgctcacg ctggacggca tcgggaatct gccctgcaat ccgtccatcg
     8941 gaggcacggc caaggggcat cttgtgcggg agatcgacgc gctgggcggt gtgatgggga
     9001 ttgcggcgga cgccactttt ttgcagagcc gcatgctgaa catgggaaaa gggccggccg
     9061 tccattccct gcgcgtgcaa acggacaggc gcaaatacca cgcctatatg aagcagctgc
     9121 tggaaaagac gcagaatctg gaagtcaagc aggccgagat cacggctgtg gacatccggg
     9181 acgacgcggt gtgcggagtg tgcacgcatc tgggcggata ttacagggct gacgccgtga
     9241 tcgttgcgac aggaacttat ctgggcggcc gtatttttgt gggcgaggcc agctatgcca
     9301 gcgggccgga cggccagcat gccgcctgcg cgctgacaga gagcctgcgc gccgcgggcg
     9361 tgccgctgcg ccgtttcaag accggaacgc cggcccgggt acaccggcgc agcatcgatt
     9421 tttcaaagct cacccgccag cccggggacg agagcatcca gcctttttcc tttctgacaa
     9481 agagcgagat gaaaaataag gtggattgct atatagcctt caccaatgag gagacgcatc
     9541 gtgttatcca tgcgaacctg caccgcagcc cgttgtacgg cggaaagatc gagggcgtcg
     9601 gcccgcgata ttgtccgtct attgaggaca aggtggtgcg ctttgcggaa aagccgcggc
     9661 accagatatt tgtggagccg tgcgggctgg acaccgagga aatgtacctg cagggtatga
     9721 gctccagcct gcccgaggct gtgcagaatg aaatgtaccg taccatagag ggcttcgagc
     9781 atttggagat catgcgcccg gcttacgcca tcgagtatga ttgtgctgac cctacggcgc
     9841 ttgcacccac gctggagttc aaggagatac gcggcctgta tggcgcgggc cagttcaacg
     9901 gcacatccgg ctacgaggaa gcggcggctc agggcctgct ggcgggcctc aacgcggcgc
     9961 gcgcggtaca gggcaggccg ggcattgtgc ttaaacggca ggaaagctat atcggcacgc
    10021 tggtggacga ccttgtgacg aagggcgtgc tggaccctta ccgcatgatg acgagccgca
    10081 gcgaataccg cctgtatctc cggcaggaca acgccgacga gcggctcacg ccgctgggcc
    10141 gggagattgg tttggtggac gacgcgcgct gggcggagtt caaccatcgc cgggccgtgc
    10201 gccgggagga agctgcccgc ctgacaaaag tggttgtgcg tcctgcggat gcgaacccgg
    10261 tccttgcgga aaaaggcgaa ccgcttctca caagcggggc ctcggccgca gagcttctgc
    10321 gacgtcccgc gttgcgttat accgacattg cacggatgct gccggaaaac ccggacgtca
    10381 cgccctttat cgcctattgc ctggaaacgg agatcaaata tgagggctat atcaaacgtc
    10441 agcagagcca gatcggcgcg gtgaaacggc aggaagaaac gccgattccc gaggattttg
    10501 attacgcgcc gttaacgggc ttgcgtctgg aggctcggga gaagctggcg aagatacgtc
    10561 cccgttctgt ggggcaggcg gcgcggattc cgggcgtcgg cccggcggat gttgcctgcc
    10621 ttgcggtgga actggcgaaa cggcgcagaa gtgagggaaa ggaataaaag ctatgattga
    10681 cacagagcgt ctcatccgga aggcggcggc ttacggcatc gacgcagctc ccgtggtacg
    10741 gaagctggac gaatatgccg cgctgctggt ggaatacaat caaaaagtaa accttaccgc
    10801 cattacggat gccgaaggca ttgaggaccg tcatttcatc gacagcctgc tgctggccgc
    10861 gcgcccggag gtgtgcgggc gtatggtgga tgttggcacg ggcgcagggt ttcccggcat
    10921 tgtggcgaag ctgtttcgcc cggagctgga tctgacgctg atggagccga cgggcaagcg
    10981 ggtcgatttt ttgaaatatg cctgcagtgc gctgggcgtg gaagccgaat tcgccaagga
    11041 gcgcgcagag gaagccgcgc gcaagcaatg gcgcgagagc tttgacgtgg ccacggcccg
    11101 ggccgtggcc gcactgccgg tgctggccga gtattgcctg cccttgctga ggcggggcgg
    11161 cgtgttcatt gccatgaaag gcagcggcgc ggatgaggaa attgatgccg cgcagggtgc
    11221 ggttgccaag ctgggcggga aatatgcggg ccgtcaggat gtgacgttgc ctgacggttc
    11281 ggcccggacg cttattttgt atgaaaaaat atcgcaaact ccgcctgttt acccaagaaa
    11341 cggcggcaaa atcgcaaaag ccccgttgaa atgacatccg gttgtcgaat gatgtaaatg
    11401 gaaataactg gaaatattag cgtatccgtg ctacaatttg tcttgttgca agatgttggc
    11461 gcggatacgc ttttttcttt tcccttttcg gcgtatttcc cgcgtgcaaa ggaggaaccc
    11521 gatttggcga aagagaaacc agcggaacag acgaaggtat ggctgcttcc cattgaaaaa
    11581 atcacaccgt cgccttttca ggcgcgcaca gtatttgatg aaggcgagat caaaaaactg
    11641 gcggtgagta ttttgcaaaa cggactgctg cagcccatca gtgtccgccc tacagtagac
    11701 gggcggtatc agctgattgc cggggaacgc cgcctgcggg catgcaagct gacgggtatg
    11761 gaaacgatcc ccgcgattat ctatcatttt gaggatgaaa agacggctgc tctgagtttg
    11821 ctggagaacc tgcagcggga acagctgggg ccgtttgaac aagcacgtgc attacgtgac
    11881 ctgttgaacc tttggaattg cacgcaggag gcgggtgcac gcaggctggg tattgcgcag
    11941 cctacactgg ccaataagct gcgcctcttg actttgacgc aggaacagca ggatatctgt
    12001 accgaagcaa acctgacgga acgccatgca cgggcggtgc ttcggctgcc gacttcagag
    12061 ctgcgcacaa aggcgcttcg cacagcagca gagcggggtt ataatgtaca gcagaccgag
    12121 gcgttggtgg atcgggtcct ttctcacaaa tcaaaaccga agcgcagtgt tatggtgcgg
    12181 gatgtgcgta tttttgtaaa taccatagac cgtgccgtaa agatgatgac gggaagcggt
    12241 attccggcaa cggctgaaaa gcgtgaacag gcggattaca tagagtatgt ggtgcgaatt
    12301 ccgacacacc ggcccgtcaa ttgaatattt ttaagattaa gccggccgtc ttaccgtaca
    12361 agagcctctc acagtcgtgc tcctgtgaat tttgtaaagt ttgtatacgt ccctttaaaa
    12421 ttgcgttctc cttcttctcc atccaaacgc cgggcggatg ttccacgtgg aacatccgcc
    12481 cggcgttttt atatggattg ttccacgtgg aacaaatgtt ttgcaggaaa aggaaaatgc
    12541 atatgttcca cgtggaacat tttgcggaaa aggcctttta aagcccccca agctgtgtta
    12601 tactaaaaca cagcggtgta agcagaaaaa gctgccttta aaatcatagt gttgaatgta
    12661 tacagaagac cggagggaaa accgttggga aaagtgatcg cagtcgccaa tcaaaaaggc
    12721 ggggtgggaa aaaccacaac agccgtaaat ctggcgtcca gtgttgcggc gcagggcaag
    12781 catgttctga ttgttgacct ggacccgcaa ggcaatacta ccagcggctt cggcgtgaca
    12841 aagcgcagcg tagaaaccag tgtgtatcag cttttaaccg gggaggcacc ggcctctgcg
    12901 ggcattcagc acacaaaata ccgtgtggac ctgattgcct ccaatatcca attgtccggt
    12961 gcaggcctgg aattggtaaa tatgcaacgt cgggaatccc gtctgcggga agccttgact
    13021 ccggtcctga atcaatacga ttttattttt atcgactgtc ccccttccct ggacctgctc
    13081 actctcaacg ggctgtgcgc ctgcgatacg gtattggtcc ccattcagtg tgaatttttt
    13141 gcgctggaag gtctgtcgga gctgatgaac accatccgaa atgtcaaaaa gatgtataac
    13201 ccttacattg agatagaagg cgtactgctg acgatgtacg acggaaggct gaatttgacg
    13261 cttcaggtcg tgcaggaagt gaaaaaatac tttggtaata aggtttataa gacaacgatt
    13321 ccacgcaatg tacggctttc agaagcgccc agcttcggcg cgcccatcaa ttactacgac
    13381 ggcgcgtcca aaggtgcaga agcatacgct tatcttgcgg cggaattcat caaaaacaat
    13441 aagaaaggcg gtgcgtgacg catggcaaaa aaaggaggcc tcggcagagg tcttgacat
//
LOCUS       DAWBSB010000030        13321 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1133_length_13321_cov_3.146088, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000030 DAWBSB010000000
VERSION     DAWBSB010000030.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 13321)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13321)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13321
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1133_length_13321_cov_3.146088"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    7345..7354
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9114..9123
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2719 a         4232 c         3522 g         2828 t
ORIGIN      
        1 accgccgcag ctatggccgc atccggcgcc tgcgatacgt cccgtgtcga cgctcagaca
       61 ctgcgcagcc ttctgctgca gcagggagct tatctggggc aatagcttgg tcacacgcaa
      121 aagtctcccg gcgggaggct tttgcattat tcattaaaga aagcgggcgg cagctgaaaa
      181 tgctaccgcc cgttttttca taccgtcatt atttgctgta caaatctact ccttttgatt
      241 ccagcgccgc ctgaagtttt ttcacatcca gagtgcgggg cgtacagcct tcccgaacac
      301 acagcgccgc cgccatcccc acagcctcgc ccatcgccat gcaaattgac atcacccggt
      361 aagatgcatg cgcccggtgt gtgcccgaaa tgcaccgtcc tgcagtgagc agattctcca
      421 ccttgagcgg cacaaaacag cggtaaggca agtcgtaggg cattacatac tgtatccttt
      481 cctccgcctg gcccgctcct tccggattat ggatatccac gatgaactcg gccttgtgta
      541 ccgccacatc agaaaagcgc tttcccgccg ccaattcttc cgccgtaaca gtatactccc
      601 ccatcacacg gcgtgtttcc cgcacgccca gcgtagcgcc gctggcaatg cacctgcatt
      661 tttcataccc gggcagattc tcccgaaaga aatgcaccag tatgtcgatt tggcggcgca
      721 gctccagctc cgaggaataa agcgcacgca cgcaagtagc gtctatgccg ttgcgctgtg
      781 tcgtgttcac ctgccgctgt ccgggatgcg tggtacggtg cagccgcaca gccgccagct
      841 gctccggcag cagcccctgc cgcgcgcagc gcgcacagta atcaagaaac cgctcaccgt
      901 tcagctccac atcgtctacg tcgccaatac atgccacagc ccggctctcg tccacaccat
      961 ccagtgtaaa ttccagcgtc accggctgca tcagtccatc ggcgcgcccc ttttccacag
     1021 gagcgcccgc aaaccaggcc acgtctccat ccccggtggc gtctatcacg attttcccct
     1081 ccatggcgcg cagcccttcc ttggttgcca ccagcagccc ttccagagag gcgccagcca
     1141 taagcgcgtc cacgacgggc gtctgcagaa acacatcaag attctcacat gaaacaaatt
     1201 cggccagaac ccgtttggcc tgttccatat cgtgggcgac gcccacctct ccgatcatat
     1261 cattctccgg tacccccagc aaggccatca gctcatcccg catggtccca ctgccgacca
     1321 tacccaatat cggcccgaca taaccggccg tcagatttcc tcccaatact ccgtaacgtt
     1381 ccaccagcgc cacgcgcgcc ccgctgcggg ccgctgccac cgccgcacat atccccgccg
     1441 ggcctgcccc tgctaccacg acctcataag agcccagcac cggtacgttt tttttgtagt
     1501 cgactgtcgt catcctcttc cctcatttcc tatgcccatg caatctattc gcctgctttg
     1561 ctttttgaaa gccctgacgc tcctttaaat cagcgcaccc catttgtgca gcatggcctg
     1621 cagcgtgtgc acatcggctt tgtgcacgct cgtgcccgca caggcacact gggcggcggc
     1681 acagcccgcc gcctggccca gccccatcac cgaggcctgc acccgcaccg aggctgaagc
     1741 cacctggtcg gctgaaaaac aacgccccgc caccagcaga ttgtcgaagc ccgggacgat
     1801 cagggaacga taagggatat aggccgcgtt ctttagaaac tggcacttct gcgtcgtgtc
     1861 accggcggta tggatgtcaa tgggatgcgc tccccggctt acggcgtctt caaaatgtac
     1921 ggcattcaaa tattcttctc cggtgagaac atgttcgcct tttatatgcc gggtctcgcg
     1981 cacgcccgtt tgcgcggcgg taaacagcag ttctgcgttt ttgaattccg gaaaatattt
     2041 cttcagcaac gctacaaaag cgtgtacgtc ctccctcagc ctgcattcgg cacgggtcgc
     2101 ctgggcaggg tccagcatat ccgcgggcgt cctggtcatg ttcaccatta ctctgccgtc
     2161 gcggtgaata ctgcaaaacc aagggccgcc gaatactgga acatcctccg tctcagccaa
     2221 ttcacgcagc tttgtctgga cgcgcagatt gtgataattg agcccctgct tttcatgatg
     2281 gatcagctcc agagcgtccg tatccacgcc gccgatacaa aagcacaggg aaggcggctg
     2341 aagcggcatt gtattttcct gcatcggcac acccgccata gcgcagagat cgccgtctcc
     2401 ggtgctgtcg atcacatatt ttgcctccag cgccctggga ccgcttttgg tgtccaaaat
     2461 cacatgcgtt atcctgctgt cctcccggac gcagcctgtg agcactgcgt gaaaatgcaa
     2521 atcaacgccg ctctccagca gcatccgctg cgcgatcaat ttatagcatt ccggatcatg
     2581 ggaaatattc cccaacggat gctctacctc cgctccgtcg gcttccacca tacgctgtac
     2641 gaattcccag ggaatccccc cgcataccag ctgtccgttg taggtgaata cgctgatcgg
     2701 cactacaagg gccgccgtag ccataccgcc cacaaaccca tagcgctcta ccaacgcggt
     2761 acgggcgcct gcgcgcgcag cggctaccgc tgcgacccag ccaccggggc ctcccccgca
     2821 cactacaacg tcatagcttc ccatcacagg gacgctgcgc gtctgcacga tctgttccat
     2881 ctccgcatca ctcctttttt gttctgtttt catcatactc tccggtgatt gcagcaggca
     2941 agaatctgga cctatctttg aaaaaaagcg acagaatcat aacaaagcaa cataaatgac
     3001 cgcgcccggc cgttgttacg gccgggcgcg gtttagtttt gttttctgcc gctacggcag
     3061 gctcacgtcc tgcgagcttt tcagatactc gcgcagcgtg tgccctgtct cgcgcttgta
     3121 aaacttggaa aaatagtgga tggagctgaa gttgaacaga tccgccagtt cggtgaaatt
     3181 tttgtccatt tgacggatgt gatactcgat acgccccacc acgcttttgc gatagtactg
     3241 catcacactg tatcccgtca cctcacggaa cagctttttc agctgcgtct cgctcaggtt
     3301 gaaatggctg gcaacgtcca caaaggtgat gttgttgtag atgttctgat ccagaaaggt
     3361 gacgacctgg cgtacgatct gatactccat ccccagagac tgcatgggca gcgactgcac
     3421 ctgtgctggg cattcctgct gcttgcggta aagcgagatc agcagctgct ccagcagatt
     3481 gcgcatcatc tgctttgcgc cgggcagatg ctccggttca tgcggcgcat ccttccggta
     3541 aaccagattt ttgccgaagg gcttatcgta tacctgtttt ccttcctcga tcatctcccg
     3601 gaacagatgg cgctcgtgca tgctcagcgt aaaaatatgg tccctgaact gctccagcat
     3661 ctccagctcg ccgttgaagg ataccacaaa gatgtcgcag tccgcgccat cctcagcgtc
     3721 gggcgcgccg tccgttcgga agttgtggaa aacattggga tggtgaaaaa tgatctgccc
     3781 tttttccagc gtatagggct cggtgccgtt gatgccgctg gcacgcccct tgtccacata
     3841 aataatttcc cagaaatcat ggctttctcc cgcaaagcgg taaccgcttt catggtgaaa
     3901 ataatacagc gtataaagct cttttacgct gagtactggc tcgacaggta ccggcttgag
     3961 catcgtttct catcctttga ctgcgccggc cgttgcgccg gacatgatct gcttggacag
     4021 cagcacataa atgatcaccg tgggcacgaa tacgatcacc acacccgcga acaggccctg
     4081 ataattgcct gtcgtctgca tactggtcac cagcgtctgc agtgccacgg ccaacgtgcg
     4141 gttggcctgc ttgccggaga acaccagtgc ccagatatat tcattccagt atccgatgaa
     4201 attgaagatg gtgatggtca cgatccccgg ttttgccagc ggaaggataa gcctgtaaaa
     4261 cgaggtgata tggctgcacc cgtcaatgag gccggattca aacaccgtca tgggcaccgt
     4321 ggcgaaaaag cccatcagat acaccgttgt cgcggcaatg cccgtgcaca ggtagaccag
     4381 gatgagcgtg ccgcggaaat tgtgcaggtt cgcccgaacc agcatcatgt acagcggcgc
     4441 caggatcatt acgcccggca ggcccagcgc aacggagaac agtgtctgga acaggcggcg
     4501 gccgcggaac acgaacttcg tcatcacgaa agccgcgggc gcgcagaaga caatgagccc
     4561 cgcacaggcg cagaccgtgt acagcacgct gttgaggaaa taatccatga tgtgataccg
     4621 ttccatcacc acttcatagc cgctcaggtc cagcccggcg gccagcaggt cgttttggaa
     4681 gatgtcccgg gtgggcgtga aggacgccag caccacccag ccgatgagtg cgaccgtgaa
     4741 ggcgcaccag cacaggataa gagccttgcc cagaacgtgc actgcctttc ccgcccagcc
     4801 gctgacgcgg cctgcgggag ggcttttctg ccgcaggcag aaagacggat atttttgcat
     4861 aggggccgtc ctccttacat ctcgtatttt tccttcggga agatcagatt gatgagcgcc
     4921 atcgccgcga cggtaatcag gcacagcacc acgccgatgg ccgcgcccat gcccacgttc
     4981 gtgcccgacg ccaccgcatt cgagctgatg gacgtaccga agatctggtc gtacatatag
     5041 ctggcgggcg tgatggtcgt ggggtccgac gggaccacgc tgaacacgcg ggaccacaga
     5101 aagaagccga tgcagccgat ggtccagaag gtgaactgcg ttttaacatt gtccgcgatg
     5161 agcggcaggg ggatgcggaa aaaaatcttt ccttccgtcg cgccgtccat aaacgccgcc
     5221 tcgaacaggt cgtccgggat ctttttcatg gcggccatgt acaccagcag gttgccgccg
     5281 atgctgcaaa agcagaaagc gatgagcatg gaccagaacg cgtattgagt gcttgtccat
     5341 ggtatggccg caaggccgtc caggcccagc gcctcgaaca ccgttttgag caggccgtac
     5401 cggctgttgt acgcatactg cgtccacacc gtgatcatgg ccacgggcgt gatgaccgtg
     5461 ggcagataga taaggttgcg ccacaggttg cccatgaaca tgcccttgtg cagtacccag
     5521 gcaaaaaaca gcgagatcag aaataccgcc gcgcctcccg ccaggaaaat cagcatcata
     5581 ttcttgaacg atacccggaa cagctgacgg ctgaacaggt ctacataatt ataaagccct
     5641 acgaattccc aggtatcccg cgcggcgctg atatccgaca cctcaaaaaa cgacatctga
     5701 actgtgcgca gcacgggata cagataagcg atcaaaaatg cgatcaccac gggcgaaagg
     5761 aacgccagat acattctgcg cagccctttt ttcaccgctg cccctccttg ttgctttgcc
     5821 gggaagcctg ccccccggct gtatcatttg aaaaagcgga tgccggcgcg catttgtgcg
     5881 cggccggcat ccctatcctg tttccttgtt ttatccggcc ggcgtcaata tttggaagct
     5941 tcgcccgtca gggtggatac cgccttgtcg gccgtcgtct gctgggtgag caccttgttc
     6001 atctccattg taaacactgc gctcacaaac tcgctggaaa ggtcgcagtt caggctggcc
     6061 accttgtccg cagcagcgat cacatccgtg cattcggcac gggcgccggg ccactcggag
     6121 ccgacggtcg ccggagcgcc gccctgctcg gccagatagt cctgcacttc cttggacgcc
     6181 acatagcgca ggaactcctt cgccgcctcg gggttcgggg agccgctgta tacggccagg
     6241 ggaacacctc cgacggaaac gtagccgctg ccctcagctc cgtcctccac ctgcgggaac
     6301 gcgaagaacc cccaggggaa gtcctcgccc gccgtgtcgg ccacctcgga aggcatccag
     6361 ctggcgttca ggtacatggc cgcctcgccc agcgcgaatt cctgctggcc ggcgggatac
     6421 ttattggtgg ctgtctgcgg ggacataaac ggcgacagcg cctccatggc ggcaaacgcc
     6481 tgcttcactt cctcggactg ccacgccggg tcggacgcgc tggcactcat ggctgcgatg
     6541 gtatcgctgc ccaccaggcg ggccagatag ttgttgggga tcagcgtcat gtatgcgtca
     6601 tcacaggtga cggcattgat gcccgcgtcc ttcagcttct ggcatgcttc catgaattcc
     6661 gtccaggtct cgggcgtttc ggtgatgccc gcctgctgga acagatcctt gttgtagaag
     6721 aagcccacca cccaggggaa catctgcacg ccgtagtatt tgtccgtctc cagcccggcc
     6781 ttttcctgtc cctgggcgtt ggcgagattc agcaccggca gaatgctctc ggccacggtc
     6841 atatcctcct ggccgagggc cggctcgttc atcatgtccg tgatgtcaag ataacggtcc
     6901 gtgctcttat catacgaaaa attgtccagg atgtccagct gctcgccgcc ctccagcgcc
     6961 gggccggtga tgtcctggtt ggaacggccg atccactgga tctccacctc gcagtcgggg
     7021 tagtcctcca tgaacatatc agcggccttt tggatggcca ttgcctcggg ctctccctcg
     7081 ttgtacatgg accagtacac gaccttgccc gaaagctgct tgccggtggc agagccgcct
     7141 gtggaagcag agccgctggc ggagccggcg gccggcgctg aagaagaagc tgcgcccgag
     7201 catccggcga acacagccag tgccatcgtc agcgcaagac agagtgccaa tatttttttc
     7261 atttgcgttt tcctcctaaa agatcctttg gaaaaggctg cgtcgccctt cctgttctgc
     7321 actttcagta taaaaaggac gccgnnnnnn nnnntttact gttttccggc accatcaaaa
     7381 aatattataa tatttttgca gcaaaatgaa caaagaaatt taaatcattt gcaaaacaga
     7441 gtcctttttt attcattctg ttttcaatat tgaagcatca aaaaacgatc tccttaacaa
     7501 aaaagacaaa ttccgaacta tgctcacagc tccacgggca ggctgcggcc atgttccagg
     7561 cgggagcgct cggccagaag cgccaccaga tggctttcga tggagtgttc cacagaagag
     7621 agcatctcgt cgtcccgccc gctggccagc agttctacaa actgtttcac cataacggcg
     7681 tctcctccgc catgcccggc catggaaccg tccgttacat tcaggtccat ttcttcgctg
     7741 tggccgaaga agtcgtgcca caccaggtgg gagagatcca tatttccctc cacacagccg
     7801 tgggtaccca tcaccttgat ggtgcggtag caccgttcgg tgaacgcgga catggtaaaa
     7861 ttgatggtgg tgccgccgtc cagctcgata tttaccacct ggtggtccac aacgtcgttg
     7921 tcgcagtgat aaacacagcg gccgtagggg ccggtgcaca gcgcctgctc gatgttctcg
     7981 ggtgtggggt gagggtccag cacgttaatg ggccagtgca cgcgccccgc gcgtacgctg
     8041 gtgcggccgc tctccagata ataccgcacc gcgtcgtagg gacagccggc gcgcacagcg
     8101 cagtccatgc agcgctgcgc cgcgcccgcg ggggcttttt ccgcacggaa cagataattg
     8161 ttgccgtaag agcttacata gcgcgcccgc ttgcccgtaa gccagatgag aatgtccata
     8221 tcgtggcagc actttgccag gatcatcggg ctggattcgt ccgctcggcg ccagttgccg
     8281 cgcacatagc tgtgcgcctg atgccagtag cccacctgtt ccatggcctg gatggttacc
     8341 acctcgccga tgcggccgtc ctcgatgatc cgcttgatgg cattgtaaaa aggcgtatag
     8401 cgcagcacat ggcacaccac cacatggcgc tgatacctcc gcgccaaacg cagtacctcc
     8461 aggcagccct caatgtccac cgccactggt ttttccagca gcaaatggta cccccctttc
     8521 agcgcggcct ccgcatggac gacatgctgc cggtcctgcg tggcgatgac cgccgcgtcc
     8581 gccagctgcg gctgcgcaaa cagttcctcc gccgttgcga aacagcgctc cgccgggatg
     8641 ttccactccc tttgcgccag ctccagccgc tcgggacgcg ggtccgccac tgcagcgatc
     8701 tccacctcat ccgggtgctc catggcatag ttgccgtaaa tgtcgtggcc gcggccgccc
     8761 aggcctgcga tgaccatcgt gattcttttc attgcataac catccctttc ccttttcccc
     8821 gttccggctc tttcgcgggc cgcggcgcgg gggcgcctgt tctcaaaagc atttggatgc
     8881 gcttgcgatc tcgcgccaga aatacacgcc gtgcaggcgg ctggtgccgg gcgtgcgcag
     8941 caggagcagc cgcccctcct ccagctcctc caggcgctgc acgctgcctg cgcaccagtc
     9001 ttccgttcga gcggcggccg cttccaggcg ggcctgtacg ccggccagcc gcagctccag
     9061 cacttcaaac ccctgcggac ggcattccgc catccacagc tgccgccagc atgnnnnnnn
     9121 nnncctctgc ggcctgccgc gcctgtccgg ccagcgctgc cagccgggcg cggtcctgct
     9181 tttggtaagc gcggtacagc ccaaggccca attccgcctt gtttttcagc acgcgggcca
     9241 gcagctcata aaaacggtac agcagcgtcc actggccgcc ctcctgcgca tacgcggcaa
     9301 actccgcctc cagcgaggcg tagtgctgcg caaagcccag cccttcgata tccaaatcgt
     9361 acaggcccaa aagcgggtct tgatacagca gaaattttgc ggcattgggc agatcgcttt
     9421 cagcgtccag aagcggcgtt ttgttgaacg cgctgatgcg cgccagcgcc cgcccgtccg
     9481 cgcccgtgca ggcggcgagc ctgcggcaga tgtgcccgcg gtccatcccg ccagtatagc
     9541 agaactccgc ccaggcctgc aggcccagca gcatcgcctg agggctcgtc tctccgccgt
     9601 cgtcgcccca tgtggtgatc atcacatccg tcacgccgtt tccgaggcag gcacgcagcg
     9661 cgggctccgc ttttttgaaa aacacgtcat agtcaacggc cgggcccagc cacgtccaca
     9721 taccgcccgc aaaccgcagc ggcgcggcaa atttctggtg caggcggatg tatctgtcgt
     9781 agaacgcctc gtcgtcgtgg taataatccc aatataccag cgtcatgtcc gcgggcgcgg
     9841 cgtctattac cgtctggggg atctcgatgt cgtcctcgta gtaatcatcg ttggggctgg
     9901 cgcagcggaa atacatgtcg ctccacatca ttggctcaaa gccgtgcttt cgggccagcg
     9961 cgcccacgcg ctccacatgg cgcagcataa tgtcaaagct ctcgtgatag ccgttctttt
    10021 tcaagtaccc gcccaagccc agattcatgg cttcgtccat gcccaggtgt atttttttcg
    10081 tgcggtagca tgcacggcac cggcgcagca tggcctcgat cagccggtag gtttcctcgt
    10141 ccccggccag cagcacatcc ggcgtatccc gcagaggcgc gctgctttcc caatgcagaa
    10201 aacgttccag atgccccagc gtctggatgc aggggatcag ttcgatgccc agcgccgccg
    10261 cccaaccgtc cagcgcacgc agatcctcgg cgccgtacct tccccgctgg tagccgaaaa
    10321 acggatattc cggcagcaca taggtatcct ccgtatataa gtaagcctcg ttgtagccca
    10381 tcagcgccat gcggcacagc atccgcttga gcgcatccac cgtcatcact gcattacggg
    10441 aaaggtcaaa actggcgccc aggccgcgga acaccggcgc ctcggtctgt tcaaagggag
    10501 ccgcgccgcc tgccaggcgc tcccgcagca ggcccagccc gcgaaaaaac tgcgcttcgc
    10561 ccgcaccaga gagctgcgcc ctgctgccgt cccaggaaat gcccggcaca ccggcttcac
    10621 tccagcgcac aggtacgccg tccccggcca gcttaaagcc gcccagcacc tccagctccc
    10681 gcacacccgt ctcaaaccgc cgcgcgcttt cttccagata gatccgcact gccatggtcc
    10741 ctcctgtcct gtattgttct tttttatcat accgcaaacc gcaaaaaaaa tcagacgcat
    10801 tttttgtctt ttcagacgca tttctcggtt aaaaatcacc tgttttgctt ttttgcgccg
    10861 tttttcggca aaagtaccgc atcaaaaagc acaataccgt ttctcaattc acgtccggct
    10921 gcggccgccg ttttatcttt ttcgttcttt ttttgcatct ttggtccgtt tgtttgactg
    10981 cgggcatcct gctttctata ataagtgcaa tgccgtccgg gcatgcgcgt caaaaaacac
    11041 cggaaagaag gaatcaccat gaaaaaaatc ggctttatcg gctacggcct gcgcagcgag
    11101 accatgatga aagcatttac cggcgcgggc cttcctctcg cgccggccgc tattgcggac
    11161 ccgcgcctgg aaacgctgca gccgcagctg gcccgtgacg gccgctttgc agacacccgc
    11221 tattacgcgg acgcggagga gatgctggac cgtgaggcgc tggacgccgt gttcatcggc
    11281 acccggtgcg gcctgcacgc ccggtacgcc gcccttgtga tggaacgcgg cctgcccctg
    11341 ttcctggaaa agcccgtatg catcgatgaa gcgcagtacg cccggctgct ggccgcgccg
    11401 cccgcgcagc gaggccgcgt tatggtctct ttcccgctgc acgtcacctg catcgtacag
    11461 gagatgaagc gccttgtgga cagcggcgcg ctgggtgacg tcgtttccgt gcaggccgtc
    11521 aacaatgtgc cttacggcgg cgtttactat cacagctggt accgggacgc cgccctcacc
    11581 ggcgggctgt ttctgcaaaa aagcacccac gacatcgact atattactca catcctgggc
    11641 aagcgtcccg tctcggtggc cgccctcacc tccaaaatgt atttcaaagg caacaagcct
    11701 gcggggctgc attgcccgga ctgccctgaa taccgcacct gcccggaaag cagctttgtt
    11761 gtggaacacc gccgcaagga agagccctct ggcgagctgt gctgctttgc cagggacacc
    11821 ggaaacgaag atgtgggcgc ggcgctgttc acctgcgaag acggcacgct catttcatac
    11881 catcagacat ttcttgtaaa aaacgacgcc gggcggcgcg gcgcgcgctt tatcggcaca
    11941 agagccagcg cggagtttga tttttacaca gcacagctcc gtgtggacta ttacgacgaa
    12001 cgccataccg ccgtccatca atttcattat ccggaaggac tggtgcactt cggcggcgac
    12061 gaggcgctgg cccgcggctt tgcggaaatg ctggaaggcg cgcccagccc gcttgggctg
    12121 gagcacgggc tcgccagcgc agccgcatgt cttgcagcgc ggcgcgcagc gcagacccac
    12181 cgggaggaaa ccatcgaata cggcttctga gcggcgcttt tccgcagcgg aagcaggagc
    12241 agagcaggcg ctcggccccc gctccataca aaatagggta cggggatgct gcattcagcc
    12301 cccagcgtcc cgtctgctcc gccgggcggc atacacaccc caaacaggca agcgccctat
    12361 gcttttcagc atagggcgct tgcctcactt cagccgcggg cggccggagg atttattgct
    12421 ttttgtatcc gcacttcggg cagacgccgt tttcattcag cgcgatgccg cacagcgggc
    12481 agcggtcaat ttcatttttc gtctcaaatt ccgcgctggc agcgtttacg ccgctggtaa
    12541 aagtgagctt cgcaatgttc agcttatcct tcagcaggtc ataaacgatc ttgatcatgc
    12601 cctcaacggt catggtctcc ttagtgacga ccaaacggca gtccggatat gcggtggcaa
    12661 gctctgtctt aaacgcctcg cccttcataa cgttgttggg atgtccgttt ttgatgccct
    12721 gtttctcgta tacgtcaagg atcgcaggca gcagggggtc atcctcccgc aggatcagcg
    12781 cgtggtcaaa gtttttcaga acgtcccatg cggttttctg aatttcattg caggggaaca
    12841 ccatgttcac gccggcattc acgctgtcct ccacttcaat ggtcagaaca cccgtatggc
    12901 cgtgcaggta ctgggcttcg cccttgaacc cataaaagcg gtgtgcatat tgcaggtcta
    12961 atgttgtaat gcttctcata acttactctc cttcttcttt ttatattatt tacgggggtc
    13021 tccgcgcccg tatgcgcatc ttttcgcgtg ataccatgtc cttgcttccg ccgcgtccca
    13081 gacgcctgta tgtataggct ccggctgttc tttgggcagg atgtcttttg agttaattta
    13141 attatatatt aaataatatt tattgtcaat agatagttaa aacaattagt tctttccctg
    13201 aaagcagttt ttattccgtc cccggccgac agaaaagctc aaattggaac attcaaaaaa
    13261 tcagggcaaa aaagttccaa aagcaacatt ttagtacaag agaaaagcag agcgaaagga
    13321 t
//
LOCUS       DAWBSB010000031        13160 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1146_length_13160_cov_2.953071, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000031 DAWBSB010000000
VERSION     DAWBSB010000031.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 13160)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13160)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13160
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1146_length_13160_cov_2.953071"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3911..3920
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2859 a         3516 c         4142 g         2633 t
ORIGIN      
        1 gatacaggct gaacgcggtt tccacctgcg cgggttgggc gtgcagggag gaaaggcaga
       61 ggggaaagca agacaggcgg ttgatgaatt ccattgccgc tccgctgatg cgcccgtcac
      121 cggcttccac acgcgaaaaa atcagctggt tgtgcttcgc ggtctgtccc tccagcagaa
      181 aggccagaag ctggcgccac tgcgtatcgt ccaacgcgtt caggttttgg aaatacagcg
      241 tgttttccgt atcgcaaagc ggagagctgt ggtgtcccag cagataattc caggttttac
      301 ggctcagcag gttacagtcg atctgaataa aagggtggct gcgacgcgcg ctgcgcaggt
      361 agattgtgcg cgccagatat tccttccctg ttccggcggc gccgaacaga aacaccggtg
      421 cgcgtcctgc cgccgcctgc aggatgcttt gatgatattg ggcggtgagg ccggtgatgt
      481 tatagatacc ggcggagcac gcctcctcca attggctgca gttataatag ctgatgccgt
      541 atttgtcccc ggcgacaggg ggtgtggaag cgagaaaata aaaggcggta taggcctctc
      601 cgtcggaggg aagctgcatc gcttcaatgt tgtacagcag cccgcggaac tgctgcatga
      661 ccttgatgcg tcccgcggcg gccacgctct cgattttttc gcgcagcatg tccagcagct
      721 ctgcggaggc gtcctccagc gtggaaaaaa ccagcttgcc cgcacgggtg aatactacgg
      781 tctggctggt gcgtttgtgc aggagctggc gcaggaactg gttttcgctg cgcagctggc
      841 ggttggcgct gcagaaaaaa acagcctggt cgaacgcggc gcggatgctt tcggcgctgg
      901 aggtgataag gaaagcgttg acgtccatct ccttggcggc ggtgtaggtg accatatcgc
      961 acaggaccga ggcatattcc tcccgccgca ggtattgcag cgtggtaacg gcctggctgg
     1021 aggattgtat ggtaaatacg tccagctggt atggcagcag cgtccgcagg atgcccatat
     1081 cctgtgtgat gttggaatat cccacaatgg ccactcgccc ctcgtgaatg ttggcgcgct
     1141 gaagcgtaca caggacatcg tacacggtgg tgccgatctc gatgaccggc agcgaaacgg
     1201 attgtttcag cagtctggcc gtgccgcccc gggagatgat cacatcaaaa tttgcgtgga
     1261 agcctttctg cgcgagctcc agccctttgg ccagatcgcc cacgctgatg gtcagctcca
     1321 ggccgtcgta ttctttcgcg gtctctgtta aaagcggcac catcccggga tagggcgaaa
     1381 tcgccagtac tttgatggat ggcattttct ttcatctctc ttttgtttaa aaatgaaaca
     1441 catttttgct ttattatagc gaattcacaa agaaaacaca acaaattttc ttaagtgttt
     1501 cataataaaa cgatttgcga agggattctg cataccagca taaaaaatac gcctgtataa
     1561 tgaaaatgac gaaaggtccg ctggacaggg ataagaggag agcctatgat acatttattt
     1621 gtaattgcgg atgattttac aggcgcgctg gatacaggcg tacagtttgc ctcctatgga
     1681 gcggccacga aggtcgttgt ggggacaggc ctgcaggcac tggaggcaga cgcgcaaaca
     1741 caggtgctcg tggtggatgc cgagacacgc caccttccgg cggcgcaggc atatgatacg
     1801 gtctaccgcc tggtgcgctg ggcaaaggaa aaaaagatcg gatgcatcta taaaaagaca
     1861 gattccgcgc tgcgcgggaa tatcggggca gagctttccg ccgcgctggc agcggatgga
     1921 ggcgacaggc tccatttcgt gcccgccttc ccgaatctgg accgcgtcac ccgcggcggc
     1981 gtacactaca tcggcggcgt gcccgttgcc caaagtgtgt ttggaaagga cccctttgag
     2041 ccggtgacgc attcccgtgt ggaggatatt ctgcatgaac agacggatac actgtgcgcc
     2101 tatatccccg gcggcggttc cgcagagggc aaagcaggcg tgctggtcta tgaagctcag
     2161 acgaaccggg atatggcaca gatcgccgac cggctggcgg cccggagcga gaccggactg
     2221 ctggccggct gcgcgggctt tgcatccgtg ctgccgcggc tgttgcagct gtcctgccgg
     2281 gacaccgcgc cgctgccgct ctgcggccgt ctgctcgcgg tctgcggcag catcaacccg
     2341 gtctcgctgg agcagtgcgc gcaggccgag gcgcagggag cgccccgctt cagcctcacg
     2401 ccggagcaga agctctcgcg ggactgggcg gccggcgcgc aggcgggcga aatgctggga
     2461 gctgtgttac gggcctgttc gcaggagccg gtggtggtgc tggacacagg cggcgcggtg
     2521 gagaacgcgg gcgcggacaa aaccgcagac cgcatccaga tcgccaatac gctgggcgcg
     2581 ctgctcaagc gtctgctgga cgacgggctg gagagcacgg tgctgatcat gggcggtgat
     2641 tgtcttttgg gcctgatgcg ccagctggat gtaacggaga tcatgcctct gtgcgaagtc
     2701 gcgccgggcg tggtgctgtc gcagttcacc tatggcggac ggcaatggaa ccttgtttcc
     2761 aaatccggtg ggtttggagc aaagacactg ttttatgacc tgcaaaacat tctggaacga
     2821 tggaaaaagg aactgcccgc ggtgtgacgg ggcatgcagc ctttcggaca ggaaaaaagg
     2881 agaagataaa atgcgtatcg cctgggaagg acgggagctg tactgccata tctcaacaaa
     2941 agcaggcttt gacccggtaa aaacacttta tgtctgcgct ccgggcgcgg atgcggcaag
     3001 ctatgaagcc gccgcacgtt ttgcgcaggc atccggctgg cgggaaatcg cagagcagga
     3061 cggggcggta ctggtgctcc ccatcgtgcc gtacggctgg cgggcggagg acgcggggct
     3121 gctgatggag atctaccgcg gcacgcgcgg cagctttccg acccaaagcg ggaaaagcct
     3181 gtatgggcgc ggcggctatt tgtggtgttg ggaaacactg atctatgcgg tgggatatga
     3241 ggacggcgcc tctttcctgg gcgacgcctc ggctgcctgt ccaagctttt ttgctgcggc
     3301 ggcgctcgtg ggcggcgtgc cgcaggatta tacgcccggg gagcgtcctt ccagccactg
     3361 gctcgtggaa aatgtgagcg cggattataa aaccctgaac cgggagctgc ccgtctgcct
     3421 ctggatgatg gtgcgtgaac ggcaggaggc ggacgcggct gtccgctatt tcaaccggag
     3481 caacggcgcg caggggccgg gctgtgaaga aatgtttgat gatatttccg cgacggtcta
     3541 ccgggcgccg gatggcggag cggcgcaggt acggatttcg accggaacct tcggcccgga
     3601 gcctgcgctg gcgcgcacgg ttttttcaca gctttttgaa cgcgtcatcc gctggaaaaa
     3661 cggcccggat ggaaagctga cgcctttcat gagccgtgcg gacttttatg cgtccccgcg
     3721 ctttgcgctg gattctgttg tgtacggggg ccgcagctac ggctttttcg ttcatgtgcc
     3781 ggaagggctg acggcggagc aggccagggg cctgccgctg gtgttcagcg tacatggccg
     3841 gggcgaaccg gcctggatgt tcgcggagaa aaacggctgg gacaggctgg cggacgagac
     3901 cggcgcgttt nnnnnnnnnn ccggaaaaca tctggtttct ggaacgcgac ggcggtgtat
     3961 tcgcccggat gatcgacagg ctccacagcc gttacggcat cgaccgggag cgcgtttatc
     4021 tgacgggctt ttccaacgga ggcatgatga cacgggaggc gggcacgcgg tatccgcagc
     4081 ttttcgcggc tttatcaccc tggaatgcac cgcctgccga gacctggccc ggctttgcgg
     4141 aaggcgggtg gcagatgccg tgcttcatct atctgggtga caacgacccc gtcgcgcggg
     4201 gcacggaaga accgctgctg gagcagatgc tccgggcaaa ctgttgtgcg gctgtgcgga
     4261 cggccggggg ctttgtgccg gatgcgatat acaacggcga aaaccattac actgccgcgc
     4321 gtgggtatac cgagggcgaa cgctttggca cagcggtcta ccgggacgcg cagggcgtac
     4381 cgcgcgtatg tgtgacagtg atgaaaaata tgccccacgg agcgatccac gatgagagcc
     4441 gggcggcatg ggaatttatg agacggtatc ggaggccggg aaacggaaag gctgtggaaa
     4501 cggtgccgca tacagagagc acagaaacag aatgcgcaaa acaaaaggag gagcgactga
     4561 agtgaacgat acgatgaacg agatcacagc attttctccc ttcggaggac gtctggtgct
     4621 ggaggaggtc aaaggtgcgc aggaacgcgc gaacgtgccg gagaacagca tcgagacgaa
     4681 ggcgggcgat gaccgcagca tgtacgtcta tgtgccggct tccggctgcc cggacgcgaa
     4741 acagacgcag gtcgtcatgt ttctgcggga tggcgcggac gaagcctccg cccaggcggt
     4801 catgaaggaa tatggtttgg atgcgctggc ggaaaaggag cattttgtgc tggccttccc
     4861 caatccgcgg caaagcggct ggagcgaaag ggacgcggaa gacatggact atctctcccg
     4921 ctgctttatg gccctgccgc agggcaaggg aaaggtcggc gggttcatcg gaatgatctt
     4981 ctatatcggc ggttctccgt cagccggcgc actgctgctt gcgatgagcg cgcgaaggcc
     5041 cctgaatgtg gcgggcgtgc tgctctcgga gctccctgcg gattacagca tcccgcagga
     5101 cggcgtgaac gcgccgcagg tggcttatct ctgcggcgga gccgcccgcg ccgcagatta
     5161 ttttggaaag gtgaacggag tgacgggagc ggacgcccgg ccgctggagc acgcggtgct
     5221 ttacaccagc cccgtaaacc caaatgtgcg gcacatcgtg tccgagctgc cgttcagcgc
     5281 gcaggaagcg gtccgtgcat gggacatgct gttctgcaaa gcgaggcgct ggtccaacga
     5341 cacctatggg acatatcaaa agcgcacgga ttttacggcc cgcggctttg tggcacacgt
     5401 caacgacccg ggcctcggcg tgaacgaagg ctttgcccat acctggtacg aatatgtacc
     5461 gccccgtctg cgcggcagca aagaaaaagc gccgctggtg ttttattttc acggcatcgg
     5521 ctgcgtgccg ctgtacggcg cggagcagag cggctggcac gatatcgccg acagggaaaa
     5581 ctttatcgtc gtatatccca agcccgcccg caacaaggcg tggaacatct gggatgagcc
     5641 gggcatgccc agcgaccagg cctttgtgct ggcgctgctg gagcatatga agcggaccta
     5701 tgccattgat gaaagccgcg tgtacatctc cggtttttcc atgggcggca tgatgaccaa
     5761 cgctctggca tgtgcatatc ccgagctgtt tgcggcggca gccccttgca acggattcaa
     5821 ttccggctat ctggtcccgg cgtcggaaat gtggacgatg ctgcggaaaa tgcccaccgg
     5881 ccgcggtttg ccggacgccc cttccgaacc ggtcacccgt acacgtgtac gggcggatgc
     5941 gaaaaaggct gcctttgcct accgtatgcc cctcatccag aattcgggtc tgctggacgg
     6001 cacatggccc gctgcgcagg acgatccctt caagcggttg gcttcttttg attactggaa
     6061 aacgtacaac aatattcccc tcacgcctta cgagcctgcc gccgcgtgtg aatccggcct
     6121 gacggccgac gagacatttt atgactgtgc ggacgggcgc ttccttcacc accgttggtt
     6181 cagccgcgat gaaggaaagc ccgcgctgtt tgaagttgtg ctcgcaaagc gtatgcccca
     6241 cgcgctggac ctgcgccagt tggagcttgc ctggcagttt atgaaaaggt tctcacgcgg
     6301 gaaggacggt tctctgcaca tcgacccgta acgcgcccgc tgcgaaacgg ccggacgctt
     6361 tgagaccaca gcagatggga ggaatggaaa agaaaagacg ggagtggaaa atggtatctg
     6421 catattggaa aacacggcaa aagcgaatag aatgaaggag tgaatcacaa atgacaattc
     6481 aaatgatcat tacattggca atcgtgatcg gcatgatcgt catgatcatg tcggacaaat
     6541 ttgccttcgg cgcaccgccg ctgatcgcct gcgttcttct ggtactgacg ggctgcgcca
     6601 ccatgtccga ggcctttgcg ggctttgtgg acaaaaatgt catcatgatc gccggtttta
     6661 tggtggttat ggcggcgttg gaaaaaacca gcctcatcga caaggtcaag gcaacgatgt
     6721 tcaatatggc cagcaagggc ggatatcgca actacatcct gttgctgatc gttgtcatgc
     6781 tgggagccag cgtgatgagc ggtacaggct attatgtatt ggtgctgagc ttggtatcca
     6841 ctatccccta caataaaaat ctgcctattt caaaaatctt tatgcccttg ggctatgcca
     6901 cttacaaccc catcgtacct gtgaatatgg cgttttacgt gggcttggtg gcctctctgc
     6961 tggagagttc cggcgtcaca ggcaccgcag tgccgctgct ggtatattcc ggcatcaagc
     7021 ttgtcagttc tatcgcgttt ttggtgtggg cggttatcgg ttaccgcttc ctgccggacc
     7081 accccattgc cgaagcgggc gcgcaggctt ccgaacaaaa gtcagagggc gaaaagctgt
     7141 ccaggtggaa agaaatcgtt gtgtatattg ctttcgtggt caacttcgta gccatgatct
     7201 tcctggataa gctgggggag ccgggctatg ccatccccgg tgtcattgcc gccgtactgt
     7261 tcgttttggg catcctcaat ttcaaggaag tgcgcaacaa tattgcctct ccgctgattc
     7321 ttatgatggc cggcgtcatc ggcgtggccg gcgccctggc gaactccggc ttcaccgcca
     7381 tggtgggtga cgcggtggcc ggcgctttgg gtaccagtgt cagcccattt gtcatcgtat
     7441 tggtgttcac actgctcacc agcctgagcg ctacgttcac gggcgccagc ttcggttccc
     7501 tgttcgtatt tgcgcccatc gccatctccg cttgcgtcag catggggttg gatcctacgg
     7561 ctgtggctgt ggcttgtgtc accgccgctt ggatcaactg gatcatgccc atcgacggta
     7621 tgcctgctat gattcttggc atgggcaaat acaagctggt tgatttctgg aagttcactc
     7681 tgccgttgta cgtcatccag attctggtgc tgtgcggggc ctgcgtgatt gtattcccca
     7741 tgtagtaata acctgcggtc ggcggcggaa catgccgccg gcccttttcg gagagagatg
     7801 atacgccatg aatacactga gtaatttgaa aatgccgcgc gccgtctgct gcggcgaggg
     7861 cgcgctgaaa aagatgccgg agatccttca ggcggcgtct gtgaaaaaag cggctgtctt
     7921 tacagacaag gggatccgca gcgccggcct gctggacccc gtgctggagc agcttcaaaa
     7981 gtcgcaggcg gcattctgtg tgctggatga cctgccgccg gagccgagct acggccaggt
     8041 ccagaagctg gtggatgcct gccgggccga gggcgcggat atgattctgg ccgtgggcgg
     8101 cggcagcgtg atggataccg ccaagctggc cggtgtgctt ttggacggca gctcgaccgt
     8161 gcacgacctg ctggaagcgc ccggccgcgc aaaaaaatgc tgtcccacgc tgatgatccc
     8221 cacaacggcg ggcacgggca gcgaagccac gcccaacgct atcgtagccg tgccggagca
     8281 ggagctgaag gtgggcatcg tcagcgacgc gatgatcgca gacaatgttc tgctggaccc
     8341 gctgatgatc aaaaatctgc cccggaaaat tgcggcggcc acaggtgtgg acgctttggc
     8401 gcacgccatc gaatgttaca caagcaataa ggcaaatccc atcagcgata cttttgcgct
     8461 ggaagcgctg gatatgattt tgaacaacat cctgcctgcg tgcgacagtg cggacgcggt
     8521 atcgcaaaaa tcagccatgc agctcgcaag cttttacgcg ggcgtggcta tcactgccag
     8581 cggcacaacg gccgtccatg cgctcagcta ccctctgggc ggaaaatatc atatcgccca
     8641 cggcgtatcc aacgccattc tgcttgcgcc tgtgatgcgc ttcaacgcgc ctgcctgtcc
     8701 ggaacgcttc gccgccgcgt atgaccgttg cgtacatgga gaaaaaacct gcgtgacgca
     8761 ggaggaaaaa atggaattta tgctgcgttg gctggagaag attgtccggc atctggagat
     8821 acccacaagc ctttcggcat tcggtgtgcc tgcgtcggat ctggagacac tggtggcggc
     8881 cggcatgcag gtgacacggc ttctgaacaa caatctgcgc ccggtgacgg cggacgacgc
     8941 ccgcgcgctg taccgccgga tcctctgaca tcggaaagga cggaaaaaca tgaatagaat
     9001 cgagatcaag ggcatcatcc cgcccatcat tacgccgatg cacgcggatg aaagtatcaa
     9061 taccgccgag ctgcgccgcc aggtggaccg ccagatcgaa aacggcgtgc atgcgctgtt
     9121 ctgctttggc accaacggcg agggatatat tctggacggc agggaaaaac agctcgtgct
     9181 ggagacggtc attgagcagg cgaacggccg cgtgccggtg tacgcgggca caggctgcgt
     9241 ttccacacgg gagaccattg cacagtcccg catggccaga gacgcgggag cggatgtact
     9301 gagtattatt acgcccagct ttgcggcggc cagccaacag gagctgtacg accactaccg
     9361 tgccgtggct gaagccgtgg atattcccat cgtgctgtac aatatcccgg cgcgtacagg
     9421 aaatgctctt gcgcccgaga cggtggcacg cctttcccgg atcgaaaaca tcgtcggtgc
     9481 aaaggattcc tcgggaaact gggataatat gaaagcttat ctggatggca cccggggcag
     9541 cggctttgca gtgctttccg gcaacgacgg cctgatcctg gactgcctga agaacggtgg
     9601 ccggggcggc gtggcgggct gtgccaatgt ttacccccgc accatggcgt ctatttatga
     9661 ttgttttgcc gcgggcgatt tggaaaaagc gcaggaatat caggcgagca tcgcgtcctt
     9721 ccgcgcatgc ttcaaatacg gcaacccgaa tacggtcgtc aaaacagccg tggcgctgct
     9781 gggctacgac gtgggaaaat gccgcgcacc gttcaaccag ctcccggaag aaggcgttgc
     9841 cgcgctgcgg caggtgcttc gtgagaatgc ggaaaagggg atgcactgac ccatgaacaa
     9901 cacggaaata aggcggccgg tcatcggcat cacgatgggc gaccccgcgg gcaacgggcc
     9961 ggagctgtgt gtgcaggccc tgctccacaa agcgatttat gaggaatgcc ggccgcttat
    10021 cgtgggggat gcaaacgtta tcgaaaaagc ggctgtgctg ctgggccatc cggagctgac
    10081 cgtgcaccgc gtggacagcg tggagcaggc ggctttcacc tgggggatca tcgacgttta
    10141 ccatctggac cttgtggacc tggcgcggtt tgaatacgga aaagtgtccg cgatgtgcgg
    10201 gcgcgcggcg tttgagtgcg tgcggaaagt cattgaactg gcactggacg acgaggtgga
    10261 tgccacctgc accaacgctc tgaacaaaga ggccatgaac ttggctctcg cgcccgaggg
    10321 catgcacttt gacggccata cggaaatcta tgcccactat actcacaccg agaaatatac
    10381 gatgctgctg gcgcaccacg atctgcgtgt ggtacatgtt tccacgcatt gctcgctgcg
    10441 ggaagcgtgc ggccgtgtga aaaagcagcg tgtactggat gtcattcata tcgcggacag
    10501 cgcatgcaga agcttcggaa tcccgcgccc gcgaatcgcc gtggcagggc tcaaccctca
    10561 tgcgggagaa aacggactgt tcggcaggga ggagctggac gagattatcc cggcgatccg
    10621 tgccgcgcag gacgagggac tgtgtgtgga cggccctgtg ccgccggaca gtttgtttcc
    10681 caaggcgctg ggcggctggt atgacatcgt tgtggccatg taccacgacc aggggcacat
    10741 tccgctcaaa accgtcggct ttgtatatga cagggaaaaa caggcgtgga aggctgtcga
    10801 aggagtaaac attacactgg ggctgcccat catacgtacc tcggtggacc acggaacggg
    10861 tttcgaccag gcaggcaaag gcacttcgaa tgaattgagc ctgctcaacg ccattcaata
    10921 tgccgtcacg atggcccgca accgcaacat caaagagtga ggggaagaaa accatgaaat
    10981 atccatgttt gcccggcctg acaccggacg gcaccgttta tcagaattct gcaatgggga
    11041 cggtggaggc catgctgccc cccgggccct gggctacggc gcatgcgccc gccttgctgg
    11101 aaactccgga cggcagtttg ctgtgcgctt ggtttgccgg cagctttgaa ggcagcggcg
    11161 atgtgagcat cgtttgcgcc cgcctgccca aaggagccgc acgatgggaa gctccagtac
    11221 aggtgtccca tgatgaaaaa cgcagtgagc agaatccctc gctgtttctc gcgccggacg
    11281 gcgctgtatg ggcggtttat accgcccagc tggaccgtgt gcccggcaag gataatatgc
    11341 agttcacgtc tgtcatccgc cggcagaaaa gtacggacgg cggccgtacc tggggcgaac
    11401 cggatgtgct gtttgccagg gagggaagct tctgccgcca gcccatccag gtgcttgcaa
    11461 gtggacgctg gatattcgca aactggctgt gcagcgacag cgcatccggt ctggccggag
    11521 acccgacggc gtttcagatc tccgacgatc agggctgtac ctggcgcact gtagagatgc
    11581 cgggcagcaa cggccgtgta catgccaatg tggtggagct tgcgccggga cgtctcgcgg
    11641 cgttcatgcg gagccgtgcg gcagatttca tttatcgcag tgaatcgctg gacgacggca
    11701 atacgtggag cgaaccggcg cccacggtcc tgcccaataa caactccagc atcagcgccg
    11761 tcaagctgca aagtggacgc attgccgttg cctataaccc cacccatacg cctgcgccgc
    11821 agccaggtgt ggcagcctgg cccggccttc gctgcccggt tgcggtggcc ctcagcgagg
    11881 acggcggcct tacatggccg atgattcgcc atatggagct gggagagggt tttgtagggc
    11941 cggaaaacag taccaacaac cgccagtatg agtacccgta tctgatgcag gcttcggacg
    12001 gcaggctgca tctggcgttc gcgtatcaga atcgccgcgg tgtaaaatgg atgacgtttt
    12061 cggagcagga tgttatgggt gaaaagcgtg aaattctagg cgtgtacaac ccgacagccg
    12121 ccagggtata gtgcgtttta caggcggaaa gcccgaaagc gcgctgccgc ggagcgaagc
    12181 ggtttcacgg cagtgcgtgc catttcagca acacgcaaaa atacggtaaa ttttttgcta
    12241 agttgtaaaa tcaaatgcga caagggttaa tactctatcg catttgattt ttttatacag
    12301 aaaatcaaag agtgtaacaa gagaacaaaa ctcttgtggc actctttttt tattgcgaag
    12361 aaaacagaaa gaaaggtggt tggacatgag aaaaggagcg aagcggctta attatgcaga
    12421 taggcagcgg attgagaaca tgaagaaatc cggggcaaag gttacagaga ttgcccaggc
    12481 ggtgggagta caccgggcta ctatctacaa tgaattgaag cgtggcggag agccttaccg
    12541 tgcagaaata gcacagagag cgttataaac gccgccaggc agcagggcag gaaagcgagg
    12601 aacttaaagg aatggattta cagacactta ttattgcaat gagtattccg agcggagtaa
    12661 ccgctttctg tttttggatg attgaacaga aaataaaaaa gcagcagacg gaagcggaag
    12721 agaaagaaaa ggcccgtcaa aaaaacgagg tccttatcat aaaaagcgtc atggcgtcta
    12781 ttgccctggg ggaagcggca gccaccgctt taaagaatgg acacgccaac ggagagacag
    12841 aagccgccct ggaatatgcc cgtaagatta agcatgaaca aaaggacttc ttaacggagc
    12901 agggcataaa agggatttac gaataatcag aaccaacaag ccaagcggct tttggaaata
    12961 taagaaagga gaaaagacca catgaaaaat attaactgga ccaggaaatt gacaagccgg
    13021 aaaatgtgga cggcggtagc gtcctttgtt tccatgatga ttgtagccac cgggggagca
    13081 gagaacaccg ccacacaggt aacggccctt atcatggccg gggcgtccgt ggtggcctac
    13141 atcattggcg agggtttgac
//
LOCUS       DAWBSB010000032        13057 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1154_length_13057_cov_3.313490, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000032 DAWBSB010000000
VERSION     DAWBSB010000032.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 13057)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13057)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13057
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1154_length_13057_cov_3.313490"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6385..6394
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7037..7046
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9285..9294
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11996..12005
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2757 a         3555 c         4145 g         2560 t
ORIGIN      
        1 aaaaagctgg cggtggcaaa gccatgcatc tttgcgcagc aaagtgcagg ccacgcggcg
       61 aatgccgccc aaaatttaaa acaaccccgc gacgccccgc acggccagtg ccgaggcgtg
      121 tagagcgaag cgaccgccga aggccgccgt gcgcggcggg cggggccttt tgatgtaaga
      181 atgccgtttt gtcaggcctc ccgggattcc gccggaagct ggatgtaatt gttggcgggc
      241 gtgatgccgg cgctcccgtt cagccggaac tgggaaggag aggtgtgaca gaatttattg
      301 aacacgcggt tgaactggga aaagctggtg aagccgcagt gcgccgtgat gtctgcgatt
      361 ttcatatccg tgtacagcaa aagctgctgg gcgttgcgca cacgcgtcat ctggatgtaa
      421 ttgatgagcg tgaagcccgt gacgcttttg aactggtggg acaggtagta cgggctgatg
      481 aaaaattttt tggagatata atccagcgag agctcctgcg cataattgct gtggatatag
      541 ctggtgatgg agtagatctt gtgggtgatg gaatcgtcga attcctgggg cacataggtg
      601 ttgcgcgggc aggcctgtgc gacggcgcac aggaattcca caaatttgct gtggatggca
      661 agctctgtca gcggcgtgcg gggctgcttg cccagcgtga aaatatcgtt caggtagccg
      721 aaggcggagg cgcgctctgc atcgcccagg cggtagatgg gaacctccgc atcaaagggg
      781 gccagcgcct ggcgcagcat gccgcccagg cacggcgtgt ccgcgggaat ggcgaaattg
      841 atgatcaggc gcttgcgcgg cgcgcccacg ggatattccg ttttgtgcag cagcgccggg
      901 cgcagaaaca cgatatcata gggctgcagc gcgtaataat cgccctcgat gatatgagac
      961 gccttgccgt ccagcaggat gtgcacctcg taaaactgat ggaaatgctg gaactccata
     1021 ttgatgttgt aagagcgctc gtcgtaatcg aaaaaataat acagcggcgc ttcggcgttg
     1081 ttgatggtaa tggaataggc ctgctcgtaa agcagcggtg cattgtaata catgcgcgga
     1141 gcctcctttt gcgctgtctg cggcccgttt gggagccggc ggccgccggg ctgccggttt
     1201 tcatccccat tatatcgcaa aacgcaaaaa atgcaaatag aaatgcgaat aaaggcaaaa
     1261 agcaagtggt ttttgttatt ttgcctgtgc tatactgatg ctgtaaagtt gaccatattt
     1321 ttgatgtatt tcagggcgga tactggttat ctgcctaaaa tctgtgtgcg caccgccgcc
     1381 ggaagagcgg ctccgggcgc gggaaaaaca tcaaacaggt taggaaggtc cgccgcagcg
     1441 gggcggatac aacaaagcaa aggagttttg gtgcatgcag gagcaggagg tagcgcgcaa
     1501 gctggagctg tgcgtcggca gctttaaaaa ggtgctgtac gagccggacg atattttcct
     1561 gaaaaacatg gagaaaaaca atctcgcggg agacgacatc tcaaagtacc agctttggga
     1621 gtggacgcag ggcgtgggcc tgtacggcct gtggaagctg ttcgagcgca cgaaagacgg
     1681 gcggtatctg gcgacgctgc gggaatatta cgacgcgcgc atcgcagacg gcctgcccgg
     1741 caaaaacatc aacaccatgg cgcccgtcct ggcgttgtcc tatttatatg aatacaccgg
     1801 cgacgagact tacggcgcgg tgtgcaaaga gtgggcccag tgggtcatgg acagccttcc
     1861 caaaacaaag gaaggcggtt ttcagcacat cacgtccgat acgctcaacg acggcgagct
     1921 gtgggacgac acgctgttca tgacggttct ggtgctggcc aacatgggcc gtatcctggg
     1981 ccggcaggac tacaccgatg aagcggtcta tcaatttttg ctgcatacga aatatctggc
     2041 tgacaaaaaa acagggttgt ggtatcatgg gtttacgttc catggcaacc acaactttgc
     2101 gggcgcattc tggggccgcg gaaactgctg ggtcaccatc gccatcccgc tgctgctgga
     2161 aatgctgccc atcgggcagc ctgcccgccg catccttgtg aacgcgctgg aaaatcagat
     2221 cgcctcgctg gcaaaatacc aggacggcaa cggcatgtgg catacgctga tcgacgaccc
     2281 cacgtcctac gtggaggcca gcgccaccag cggcttcggc tatggtatcc tgaaggccgt
     2341 gcatatgggc cttgtggacg ccgcctatgc ggagaatgcc aaaaaggcgc tcgcccccat
     2401 tcttaaatgc atccgcgagg acggcattgt ggaccaggtg tcctacggta ccgccatggg
     2461 ccgggagaca aaggatttct acaagcagat cccgctgcgg cagatgcctt acgggcaggc
     2521 catggccatg ctgttcctga tggaagcggg cgaagaagcc tgacgcgcgg aaggggtagg
     2581 gtatgaagca cacacgcctg acctttgcgg cctttgtggt gctgatggtg gggctcggct
     2641 ccagcgacag cctgcgcggc attttctcgc cggtattcag cagccatttt gcactttcgg
     2701 ccacgcaatt ggggctcatc gtcacggtga gctacattgg caacctggtg ttccttctgg
     2761 tgggcggcaa cgtcattgaa cggtttgaaa agaaaaaagc gctgctggcg ctgacgggca
     2821 tctggatggc ggcgctggca gtgttcgcct tcacagataa ttactatctg ctgctggcgg
     2881 gtatgttctg cgccatgggc gcgtccacgc tgctgaacac gacgctgaat ctcgtcacgc
     2941 cgctgctgtt cgcgtcgtcg cccgcgtttt ttgtgaatct tctgtttttt acacagggca
     3001 tcggcaccag cggcagccag tctctgctgg ggcgttatgc cacggatatc ggcttctggc
     3061 acaagacgaa cctggtgctg ctggcgctgg gggccgcggc ggtgctgctg ctggcgttca
     3121 gcacggtgcc cggcccgcgg gcggatggcc cgcatccggc aaagcgtcag tctggcgcgc
     3181 tgaaaaacag ggccgcgctg ccgctcgtct ttgtgttcgg tttttatttc atcgccgagc
     3241 acggtgtgat gaactggctg gtggcctacg ccaccgcggg cctcggaatg gagcaggcca
     3301 aagcatccaa ctacctggcc gtgtttttcg gcggcgtgat gctgggccgt ctggtgcttt
     3361 cgccgctggt ggacaggctg ggcatcctca agagcatggg cgtcttcggc tcgctctctg
     3421 cggtgctgta catcgcgggc gttgtgggcg gcgcggcggc aatgccgctt tgggcggcca
     3481 gcggcttttt cctttccatc ctgtatccca cgctggtcat gagcatccgc ctgtttttcc
     3541 cgcatgggca ggtggcgggc gcggccggca ccatcatcag tgtcgcgtcg ctgtttgata
     3601 ttctgttcaa cgtcggcttc ggccgccttg tggatacgct gggctacggc gtcagcatct
     3661 atgtgctgcc gctggccatg gcggcgttca tcatcacctt cctggccttt tcacgcgggc
     3721 atgggaaaga acagcggaag agctgacggc tccgcccttt gcggctatgc gggggcgctg
     3781 caaccgaagg aggaaatatc atgaagatcg gtatccgcgc gcacgatttc gggtgcctgc
     3841 cgcctgcgcg gctggcggaa aaaatggcgg cccagggcgt cgaggcggcg cagctggcca
     3901 tcccaaaggc cattgccggg gtggagagct atgcgcaggt gacggacggc ctgctgcgcg
     3961 agatcggcgc ggctttccgg gagcaccaca ttgatatcgc cgtgctgggg tgctatatcg
     4021 agccgtcgct gcccgatgag gctgcgcgca aggcgcagct ggatacgttc cgcttggggc
     4081 tgcgctgtgc ggcggagctg ggggctggct gcatcggcac cgagacgacg cttttttccg
     4141 ggccggagca ggagcgcccg gcggcttatg agatcctgcg ccgcagcgtg gacgtcatgc
     4201 tgaacgatgc ggcgaagctg ggtgtaaccg ttgcggtgga gccggttgcg gcccatacgc
     4261 tcaacagccc ggccctggcg gcccggctgc ttcaggagtt ccgcggcggg ggccttgcgg
     4321 ctattttcga cgcggtgaac ctgctcacgc ccacgcggat cgaaacgcag gcggcgctgt
     4381 gggacgaggc gctggccgcg tttggccccg atatcgcggc cgttcacgta aaagacgctg
     4441 tggtaaagga cggagcgctg gccccggcct tgctgggaga gggcgtgatg tgttatgata
     4501 acatcatgcg ctggctggcg cggcataagc ccggcgtggc gctgctgcgc gaggagatcg
     4561 ctcccgaaac agccgcgcat gaccttgccg tcatgcgtgc ctgggcgggc agataagcca
     4621 aatacgatgc gcgcagaacg tactgttctg cgcgccgttg tttacagagg aggaaaacgg
     4681 aagatggaat actgtatcgg cattgacctg ggcggcacca acatcgcggg gggcgtcgcc
     4741 acgctggacg gccgtctggt gcacacacag agcgtcaaaa ccggcatgcc gtgcacgccg
     4801 gatgaaattg cggaccgcat cgccgcgctg tgccgcagtc tggcgcaggc ggcgcagacg
     4861 ccgctggagc agatggcgtg gatcggcgtg ggcgcgcccg gcagtgtgga caaagatacg
     4921 ggcgtagtgg aatatgccaa caatctggaa ttctataatg tgcctttggg cgcgatgctg
     4981 cgcacccgtc tgggcctgcc cgtctatgtg gacaacgacg ccaatgcggc ggcctggggc
     5041 gaataccggg cgggcgttgg caagggctgc tccagtatgg tgatggtgac gctgggcacg
     5101 ggcgtaggcg gcggcatcat tgaagacagc cgcatcgtca cgggctgcaa ctgggccgcg
     5161 ggcgagctgg gccactttgt catcgatatg aacggtgaac cgtgcaattg cggcctgcgc
     5221 ggctgctttg agcagtacgg cagcgttacg gcgctcatcg cacagacgcg ggccgccatg
     5281 cgggccgcgc cggacagcct gctgtgggag caggccggcg gcagcgagca gctggtgaac
     5341 ggccgcacgg cgttcgacgc catgcgccgg ggcgacgcgt ccgcgaaagc agtggtggac
     5401 ggatatgtgc attacctgtg cgtgggcatc acgagcatcg tcaatatctt ccagcccgag
     5461 gtgctgtgcg tgggcggcgg catcagccgc gagggcgagg ccgtaatggc gccggtgcgc
     5521 gcctacgtgg gaaaatacgg ctatgcgcgc ggcagcgcaa agcagccgcg cattgtggct
     5581 gccagccttg caggagacgc aggcatcatc ggcgcggcgc tgctgggtac cgagaaaaaa
     5641 taactcccaa ttcccttatg cgttcccgaa gtaagggtag taacagctca tgtggattta
     5701 ttcattaggg gacataaaat caggctccat cctttcgggt ggagcctctg cctttgtgta
     5761 gagtcgttta tttggacttc ttataaagag tgtatgccac aacagcacat ataataattc
     5821 caatgccaaa cggaatagca gaaagaaacg caacactagc aggtgcacta tagcccaaat
     5881 gttcaatgcc acaaagcata tcgcggtaat tgaacgccac aacagagcac ataatgttcg
     5941 acagcaggat tgcaatagca ataaacaaat aactcagctt tttcatgcac gttacctcta
     6001 tttcattttt tgcgtagtat tactactaag cctgcaacca tcgaaagtac gcatagaatg
     6061 aggagtatgc cggatccatt cagagtaatc gtcgaagcag cattaagttt gtctgttgca
     6121 cttggacttg ttaccatcat gacgccggat agaatcaggc agatggcacc aatcaagcaa
     6181 agtcctatgc ctactttcat ctctgctact tttgaagcct tgttttctct tgattttagt
     6241 ggggcttcac ttgcctcttg atttgctgtc tcatccttca caagctcatc aagagtaata
     6301 ctgaagcaat cactcaatgc cagcagtttt tccaattcag gtgtagatgt tccactttcc
     6361 catttagaga tagcctgtct ggaannnnnn nnnntatctt ctcggcaagt tgctcttgag
     6421 aaagcccgct ttttcgtctg aatttatata tccgttcaga caacatttgc tttacctcct
     6481 taaaaacatt ataccgcaat ccatggttac acactaccaa ctactattga aacttccgca
     6541 accagtagtg gcacaaccaa aaatctaaaa atttgaaccc ttttttactc cataatatca
     6601 tactgctgtt atatctgtat atagagatca gaagtatcac ccatttctga atgatagccc
     6661 tgctctctat cgttgttgat ttttattcgc cgtatccaca agattataca cgaattttga
     6721 ccgggaacat aatgtcgaat tggggcaaaa ataaaagcag aagagagaac cgtgcgtgtt
     6781 ccatacacgc acggtttttt atgcagcggg tcacctatgc agcgcacgga gaaacagctt
     6841 cttaatatgg ttttaacatc agagaaaaaa tctttatgcc gtgcaaaact gcggatactg
     6901 acattccgtt cggaaaacgc tataatgaga tggatcgaag gcgccgggca gggcccggcc
     6961 ggcttgcgga agaaagggga cccggatgtt cggacggtgc ggtgcagaac aagaaacagg
     7021 acgcctgcgt atggagnnnn nnnnnnatat gggcaggacg gcgggcattc tgtgcgcggc
     7081 ctccctcgtc gggctgcttt tttacaaagc gggcatcacc gaggcaaata tcgttacggt
     7141 atatctgctc gccgtgctgt gtacggcgtt ggccacagac ggacgggtgt acggcatttc
     7201 tgcggctgtt gcaagcgtgc tggttttcaa ttatctgttt accgtgccgc gctttacgct
     7261 taagttcaat gatgtgagcc ggtattcggt cacgtttctt gtgatgttca ttgccgcgtg
     7321 catcaccagc acgctgacca tgcgggtgaa aacacaggcg cgccaggcgg cgcgcaaggc
     7381 gcaccgcagc gaagtcctgc tggagaccag ccaggcgctc cagcgcgcgg agggagaaca
     7441 gcagatattc accgcggcgg cagagcatct ttcccgcctg ctgcgtgcgg gcgtttcact
     7501 gtatccggtg cgggacggcg cgctgcagcc tcctctggtt tgcgacgcgc aagggacgga
     7561 ggccgcgccc tgtacaacgg aacgggagct gcgtgccgcc cggaccgtgc tgcgctgcgg
     7621 cggccaggcg ggggcccgta cggccgcctg cccggatgcg gaatgcctgt atctggccgt
     7681 gggtgcgtct gttccgccgc tggccgtggc gggggttgcc ctgcggaagg gacacattcc
     7741 ggatgatttt gacagaaacc tgatggcggc gctgctaaac gaatgcgcgc tggcgctgga
     7801 aaaagagcgc atcgcccgcg aaaaatcgga cgcggaactg caggcccagc aggagcagct
     7861 gcgggcgaac ctgctgcgca ccatctccca tgacctgcga accccgctca ccagcatctc
     7921 gggcaacgcg ggcgtactga tggagaattc cagcgtgctg gatgaggcga aaaaacagcg
     7981 cctgtatgcc gatatctacg acgactccat gtggctcgtc cagcttgtgg aaaatctgct
     8041 ttccatcacc cggctggaaa acggcgggct ggacctccgc atccagcccg agctgctgga
     8101 ggaggtggcg ggcgaggcgc tgcagcatct gaaccggcgc agcgccgagt atcccatccg
     8161 tgtggatatc gaggacgacc tgctgatggc gaagatggat tcccgcctga tcacgcaggt
     8221 catcatcaat atcgtgaaca acgccgtcaa atacacgccg cccggtacgg acataaccct
     8281 cagcgcgcgc cgcgagggag acacggtgcg ggtggaaatc gcggatacag gccccggcat
     8341 tccgcaggag gcacagggca ggctgttcca gatgttctat accgcgggca acgaccgcgg
     8401 cgacggacgg cgggggctgg gcctgggcct ggcgctgtgc aagtccattg tggaggcgca
     8461 cggcgggaag atcggcatgc gggccaacga gccccacggc tctgtattct tttttaccct
     8521 gcaagcagag gagataaagc tatgaacaaa gcaaaaatac tggtggtgga ggacgacaag
     8581 gccgtgcgca acctgatcac cactacactg gagacgcaga attaccagta ccacacggcg
     8641 gaaaaaggca gcgaggcgct gctggaggct gtttcctttc agccggatgt gatgctgctg
     8701 gatctgggcc tgccggatat ggacggcatt gccatcatcg aaaaggtgcg cggctggtcg
     8761 ggcatgccca tcatcgtcat cagcgcccgc agtgaagaca gggacaagat cgacgctctg
     8821 gatgccggtg cagacgatta tctgacaaag cccttcagcg tggacgagct gctggcccgc
     8881 ctgcgtgttg cgctgcgccg cgcgcggcag gacagcggac acgcgggcgc gcccgaggcg
     8941 gtgtttacca atggtgagct gacgatcgat tatgccgcgg gctgcaccta tctggccggg
     9001 caggaggtgc acctcacccc catcgagtac aagctgctgt gcctgctggc aaagaacgtg
     9061 ggcaaggtgc tcacgcacaa cttcattttg aaggaggtgt ggggcagcgc cctgccgggg
     9121 gatacgcctt cgctgcgtgt gttcatggct acgctgcgca aaaagatcga gccggtgccg
     9181 gcacagccaa aatacatcca gacccatgtg ggcgtgggtt accggatgct gcgcattgca
     9241 gagggagagc ccgtacagaa aaacggatga tatttgatgc ggaannnnnn nnnnccccgt
     9301 cggggcaggc ggcttttttt ccttcggcgg gattttgctg ttgacggaag actgtttcca
     9361 tattatacta aaatagtata atacaaacgg aagaagggga cctgatgagg aacatcatca
     9421 aacgcgacgg cacggtgcgg ccctacgagc cggagaagat catcacggcc atgggcaagg
     9481 ccttccgcga aacggcggcc gggacgagcc gggaggagat ggaacgcctg ctgcgcgcgg
     9541 tggaaaagga aatggagcac aaagacggcg gttgggccgt ggaggacata caggacgctg
     9601 tggaacgggt gctgatggaa aacggccgtt acgaggaagc aaagcggtat attttgtacc
     9661 ggcaccggcg caacgagcag cgcgcggcgc ggcgcgccat ggctcaggcg gcgggcgttc
     9721 cggcactgga cggcgtgctg gccaagatcg aaaaggattt ccccggcgag gcgtatgcgc
     9781 tgaccgtgct gaacacaaag tttcagagct ttgtcaagcc gggcatgggc gcggacgcgg
     9841 cgctggaggc gctggtgaag gcggctgtgg agctgacgag ccaggaggcc ccgcgctggg
     9901 aattcatcgc ggcgcgcctg ctcaatgcac ggtttggccg cagcctggag gagcagctgc
     9961 gcaagcgggg catcggcggc ttgtacgaca agctgcgccg cctgacggac gaggggctgt
    10021 acggcgaata catcctggcg cattacagcc gggaagagat cgagcaggcg cagaattttc
    10081 tgcggccgga gcgggatgag ctgtttacat actccgggct ggacctgctg ctcaagcgat
    10141 atgtcatcca cgaccgggcc cacgctccgc tggagactcc gcaggagatg tttctgggca
    10201 tcgcactgca tctggccatg cgtgaaaagc gggaccgcat ggcctgggta cagaaatttt
    10261 acgatatgct cagccggatg catgtgacga tggccacgcc cacgctgtcc aatgcgcgca
    10321 agccctatca gcagctctcc agctgcttca tcgatacggt gccggacagc ctggaaggca
    10381 tttaccgaag catcgacaac tttgcacagg taagcaaata cggcggtggc atgggcatgt
    10441 atttcggaaa ggtgcgcgcg gcgggcggca gcatacgcgg ctttgagggc gcggccggag
    10501 gtgtggtgcg atggatccgc ctggtgaacg acaccgccgt agcggtagac cagctgggca
    10561 tgcgccaggg cgctgtggcc gtgtatctgg acgcctggca caaggacctg ccggagtttt
    10621 tgcagcttcg caccaacaac ggggacgacc gcatgaaagc ccacgacgtg ttcccggcag
    10681 tgtgctatcc ggacctgttc tggaagctgg ccaaagagga catcgacgcc gaatgggcgc
    10741 tgctgtgccc ccatgaggtg ctgacggtaa agggctatgc gctggaggat tacaccggcg
    10801 cggagtggga gcgccgttac cgcgactgtg tggcggacgc gcgcatctcc aaacgtgtgg
    10861 tcacggtcaa ggaactggtg cgcctcatcc tcagatccgc cgtagagaca ggcacgccct
    10921 ttgctttcaa ccgggacgcc gtcaaccgcg cgaaccccaa cagccacaag ggtgtgatct
    10981 actgctccaa cctgtgcacg gagatcgccc agaacatggc gcccatcgag accgtgagcc
    11041 gggaggtgga aacacgggac ggcgacactg tggtcgtcac caccacgcgc ccgggcgagt
    11101 ttgtggtgtg caaccttgcc agtctctctc ttggacatct gcctgtggag gacgacgcag
    11161 tgctgcggga caccgtgcgc accgctgtgc gtgcgctgga caatgtcatc gacctaaatt
    11221 tctatccgct gccctatgcg gagctgacga accggcgcta ccgcagcatc gggctggggg
    11281 tgtccggcta ccaccacatg ctggccaagc gcggcatccg gtgggagagc ggcgagcatc
    11341 tggcctttgc ggacgatgtg tttgaacgca tcaaccgcgc ggccgtggag gccagcgcgg
    11401 accttgcggc ggaaaaagga agttacgcct attttgaagg cagcgactgg cagaccggcg
    11461 cctattttga aaagcggggc tatacctcgc cggaatggcg tgcgctggcg cagaaggtgg
    11521 cggcgcaggg catgcgcaac gcgtacctgc tggccgttgc gccaaccagc tccaccagca
    11581 tcctttccgg caccaccgcc ggtgtggacc cgctgatgaa gcgttttttc ctggaggaga
    11641 aaaagggagg catgctgccc cgcgttgcgc cggagctttc catggatacc tattggtact
    11701 acaaaaacgc ccataccatt gaccagaagt ggagcgtgcg cgcctgcggc gtgcgccagc
    11761 gccatatcga ccaggcccag agcgtgaacc tgtacatcac caacgaatat acgctgcggc
    11821 aggtgctgga gctgtatgtg ctggcctggg agtgcggcgt caagaccgta tactatgtgc
    11881 gctccaaatc gctggaggtg gaggaatgcg agagctgctc ctcctgaagc agccctctgc
    11941 gggcggaacg gacaggaaga acaaaggccc cgtaccgggg cgggaagagg agaaannnnn
    12001 nnnnnctgtt caacccggac ggagacaccg acgtccggct gcggcggatg atcggcggca
    12061 atacgaccaa tctgaacgat tttaacaaca tgcggtattc gtgggtgagc ggctggtacc
    12121 gtcaggccat gaacaatttc tggatcccgg aggagatcag cctggcgcag gacgtgaagg
    12181 attacccgcg cctggacgcg gccgagcgta cggcctacga taaaatactc agttttctgg
    12241 tgtttctgga ctccatccag acggcgaacc tgccgaacct cggcgaatac atcacagcca
    12301 acgaggtgaa tctgtgcctt aacatccaga cattccagga atgtgtccac agccagagtt
    12361 acagctatat gctggacacc atctgcagcc ccacacagcg tgacaatatc ctttaccagt
    12421 ggaaaaccga cgagcatctg ctgcgccgta acacgttcat cggggaatgc tacaatgcgt
    12481 tccagcgcga gaaaagcccc gaagcgctgc tcagggcgct gatggcgaac tacattctgg
    12541 aaggcgtgta tttctactcc ggcttcatgt ttttctacaa cctcagccgc aacggcaaga
    12601 tgcccggctc cgcacaagag atccgctata tcaaccggga cgagaacacc catctgtggc
    12661 tgttccgcaa tatcatcctg gagctgcaga aagaacagcc cgagctgttc ggtacggaaa
    12721 acgtgcgcat gatgcgcgag ctgctggagg agggcgtgcg gcaggagacc gcgtggggcc
    12781 attatgtcat cggcgacgcg attcccggcc tgacgcggca gatggtgacg gattatatca
    12841 ggtatctggg caatctgcgc tggacgagcc tgggcttcgg cacgctgtat gagggttatg
    12901 aggctgagcc ggccaacatg gcctgggtga gccagtattc caacgccaac ctggtgaaaa
    12961 cggatttctt tgaggcaaag agcacggcct atgcgaaaag caccgctctg gtagacgacc
    13021 tgtaagctgc tgctccgtaa aaaagaggcc ccggcag
//
LOCUS       DAWBSB010000033        12929 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1166_length_12929_cov_3.187121, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000033 DAWBSB010000000
VERSION     DAWBSB010000033.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 12929)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12929)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12929
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1166_length_12929_cov_3.187121"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6416..6425
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2423 a         4026 c         3921 g         2549 t
ORIGIN      
        1 ccctgtgctg gcgcacaggg ctttgagaac attgaaccat gcaaaaccgc agcacatgcg
       61 gttttgttgg ggccgctgcg cggcgtcagg gtataataac cccaaagccg ccgcttgcgc
      121 ggctgcacgt gtgcacggca cacgcccctg tgctggcgca cagggctttg agaacattga
      181 accatgcaaa accgcagcac gtgcggtttt gttggggccg ctgcgcggcg tcagggtgta
      241 atataccatc cggaatgttt cataaaaata tacatacggt catctttgac ggacggccgg
      301 aaaggacaat ctgcgatgca gtactccaat gtggtcccgg gacgcttcat ttcccggccc
      361 aaccgcttcc ttgcacaggt gaaaaccgcg gacggcgtgc aaacctgcca tgtgaaaaat
      421 accggacgct gtgccgagct gctgctgccc ggcgctccgg tatggctgca gcggttcggc
      481 gcggacgcaa agcgcaagac aaaattcgac cttatcgccg tgcagaaggg cgcgcttctg
      541 ctgaacatcg acagccaggc gcccaatgcg gcggcacggg aatttctggc ctccggcggg
      601 ttaggctgga ttcccagcgt actgcgcccc gaatacacct ggggcgattc ccgttttgac
      661 ttttatttgg aacacggcgg gcgccggtgc cttgcagagg tgaagggcgt cacgctggag
      721 gaggacggca cggcgctgtt ccccgacgcg cccacgctgc gcggcataaa gcatctggag
      781 ggcctttcac gcgccgcggc gcagggcttt gcgtgctgcg tgctgttcgt cgttcaattt
      841 tctcccgcag ccgccttcgc gcccaaccgg cgcacgcacc ctgctttcgc agacgccttg
      901 cgcgccgcac gggacgcagg agtacgcgta ctggcctacg actgcgccgt cacaccggag
      961 tccatggtcc tgcgcgcgcc cgtccccgtc acagggcttt gaaaaacgcg ccgcccatca
     1021 gcgtcctccc cgtccctgca tccggacggc aaaagccccg tccccaaagg ccgttcggcc
     1081 gctgggggcg gggctgcttt ttatccttta tcctgcgccg tgcacgattt ttcgcgctca
     1141 gaccgttttc tgcatggtat aaggcaggat gcccaggaac aggttatact gcacgccgga
     1201 cacattgctg tgcatggcat agacgtcctg gttcacatag gtggggatga gaggcgcttc
     1261 gtccagctcg atctcctgca tgcgcgccca catctccgca cgtttttcaa cgtccagctc
     1321 ggagcaggag gccagataaa ggctctcgca ctcatcattc tgccagccgg tatacatacc
     1381 gttggtgatt gggttgtaca caatgaaaga caatgccaag gcgctgtccg ggcccgtgcg
     1441 acccagattg gtcaggccca cctgatactc gccgtttttc ttcatggcgg taagcgtggc
     1501 cgcgtcgtat tgggtgacct ccagcgtcac gcccgcctgc gcccactgct cctttagaat
     1561 ggcggcgatc tgtgcctccg tggtattgcc ggaggtgatg ctcagcgtga gcgtcaggcc
     1621 gtcggcatag cccgcatccg ccagcaggga tttcgccttt tccacatcgt gggtgtagcc
     1681 ctccagcccg gtgttgcgca gcacgtccag attgtcggta aaggtggtga cgcgctgggc
     1741 gtacccctgg ttcgcgccta cgatcagatc atccacattg gtagcatacg ccagcgcctg
     1801 gcgcacttcc ttgctggaga ccgcctcagc ctccacattg aagccgatgt aattcaccgt
     1861 tacggtgtca aaggcttcta ccttatagtt gctgtccgcg ctgagctggg gcagattgtt
     1921 gcgcggcgtg ctcatcatca ggtcgatgtc gccgttctgg aactccatca ggcgggtgga
     1981 atcatccgcc acgacggtca ccttgatctc ctcggtaaca gccttggacg ggtcgtagta
     2041 attgggattc tttttgaaca gcatgtactg gtctttcacc cagtcggcaa tataatacgg
     2101 gccgcaggtg acgaggtcct tatagtcgcc gctctcgccg ctgtccttct ggacgatggc
     2161 gcagcagggc attgtgaaat tggacaggaa gtccggcgtc ggggcgttca gcgtcacaac
     2221 gaccgtcgtg tcgtcggggc attcaatgcc cgcgatggcc gtcagaaagc ccgcgaagat
     2281 ggatttttcc gggtcgatgg cacggttgag agaatacagg atatcctctg ccgtaacagc
     2341 gtcgccattg tagaatttca gcccgtcgcg cagatggaag gtgtacgtca ggccgtcctc
     2401 ggaaacgtcc cagctctcgg ccaaagcgcc ctcgatggcg gtgccgtcat tggccggaaa
     2461 cacaaggcct tcaaagttcc aggccacagt catcaggtcc gtggcgttgg aggtctgcgt
     2521 cgggtccaca agagcggcgt ccttattgcg tgcaacgacg atctgctgcg caatgcccgc
     2581 gtccgccgcg ccggaggagg cggacgccgc acccgaggcc gccgcgccgc cggaggaggc
     2641 gggcgcgccg ccgcagccgg caaggcccgc tgccagcagg gccgccgcca aaaggctggc
     2701 gatcaatttt ttcatattga aaactccttc acaagttttt tcttccccgc gcccggcccc
     2761 accctgttcc tgccgtaagc gcttgttttt taactggaag ttaaaccaaa ttataaatct
     2821 ccggctgcgc cctgtcaata gaaaatatgt ataaagatac tcttttttgt tttttgaatg
     2881 caaaatcggg gcttgcaaaa cgccgccgca tcccgtacaa tatcggtgaa acgaggtgca
     2941 tgttatgctg gaatccatcc gcgagctgcg gcgccggaaa aacttcactc tgcgccagct
     3001 cagtgaaaaa acagggtata ccgtcagctt cctttcccag ctggagcgcg gcatcaaaaa
     3061 cccgtctctg gacgcgctgc gggccattgc gcaggcgctg gacgtctcgc ttgtgagcct
     3121 gatgaacaat tccgattaca cggccggaag ccgcggcgaa accggcgacg acgccggaga
     3181 gagcggcttt tgcgtgctgc cgccggacgg ccggcgcaca atgcccattg atcttccggg
     3241 cgtccgctac cagcttttga acgcgcagca gcttgccccg ggccacgcgg gcgacgtgac
     3301 gatggtgctg tgcacgctgc agcccggctg ctcctccagc ggcaaaatgg tgagccacct
     3361 ttacaccgag gtatgctttg tcatcgcggg gcgcatccgc gcgctgcgcg cgggcggcga
     3421 ggagctgctc ggcccgggag acaccatccg cgtgacgccc ggcgtgctgc atgattttta
     3481 caacgtgggc gatgcgcccg ctgttgtact ggccgttaca ttttagcttg acaattggtt
     3541 tgtgtttgct ataatttact gtaagttaac tttgtcgcat taaagcggaa aagagcgatc
     3601 caattgcgaa ggctctccga cggcggaggg ctttcgcata tcttttttgt cgggggagat
     3661 gtccgtgaaa gaaatcaatt acattttaaa gcgggtgctg cagatgatcc cggtgctgct
     3721 gctggtgatg gtgctgatct tcgcgctgat ccgctttatc ccgggcgacc cggccgacgt
     3781 gatgctcggc gcgcgcgcca ccgaagaatc acgcgccatg ctccatgcca agatgggcat
     3841 cgacaagccc atctatgagc agtttttcct gtttatgaaa aactgcctgc ggctggattt
     3901 cggggaatcc atcctcagcg gcgagcctgt ttcgcagatc ctggcgcgcc gctttcaggt
     3961 gacggcctcg ctcacgggca tgacggccct gttcacgatg ctgctggcgc tgcccatcgg
     4021 ttatttttcg ggtgtgaaca agcacaacgc cgccggaaag gccataaata cctcggcgct
     4081 ggtcgtcatg gcggtgcctg cgttctgggt cggcatcctt ctgatgtatt tcttcgggct
     4141 gaagctgcac tggttcccca tctccggctg gggcgatacc ctcggcgagc atgtgtggtc
     4201 cctcatcctg ccggcgttca cgcagtcgct gggcatctcg gcgctgctca tccgcaatat
     4261 gcagaacgag gtggcgcaga ttttcaccat cgactatgtg gattttgccc gcagcaaggg
     4321 ccttgcaccc atgcgcgtgc gcgtgtggta catcctgcgc aacgtaatga tctccaccac
     4381 cacgcttttt tccatcggca tcgccagcat gctgggcggc agcgtcatca tcgaaacggt
     4441 gttctctctg ccggggctcg gctcactgat catgtccagc atcctggcgc gggattaccc
     4501 cgccgtacag ggctgcgtta tcctgtttgc cgttatcatc atggcggtga acctgctcac
     4561 cgatattttg tattccgtgc ttgacccccg cgtcaagcta cagtaaagga ggcgcgcata
     4621 tgcaggcaga aaccgtaacg gcaccgcgcc ggcgcaccgt atggggtgcg ctgcgccgca
     4681 acctctcgct caccgcgggg tgcgcgatac tgctgtgtgt ggtgtttacg gccctgttcc
     4741 cggggctgat tgcaaaccag tcccccacgg accagaacct cacggccatg ctggccgcgc
     4801 cctcggccga gcacatctgg ggcaccgacg agctggggcg cgatattttc gcccgtgtca
     4861 tctgggggac ccggtatgac ctcgtcatcg gcctggcggc cgccagcatc ccgttcgtcg
     4921 ttggtacgct catcggcctg ttcacggcct atttcggcgg cattctggac acgcttttca
     4981 tgcgccttgt ggatgtaatg atgtccttcc ccgtcatcgt cctcatcatc gccatcgtgg
     5041 cggtgctggg gcccggcatc ggcaatatgt acctggccat gtggctggtg ggatggacga
     5101 gctacgcgcg cctggtgcgc agcgccgtca tggtggaaaa aaacgctgaa tatgtgcagg
     5161 cggcgcgtac gctgggctat tcccacctgc gcatcctgtt ccggcatatt cttcccaacg
     5221 tcatctccgg cgccatcgta tacgcggttt cagacatatt gatgtgcatg ctcatgggtg
     5281 cggggctcag ctttctgggc gttggcgtac agccccccac cccggagtgg ggcgccatta
     5341 tttcgggcgg ccgttccttt ttgttcaacg cgccgtggat cagcattttt cccggcatct
     5401 tcctggccgt cacgggcgtg gggctcagcc ttttgggcga cggtcttgca aagtttttcc
     5461 aagtaaaggg gcattgacat ggataaacct gtattgctcg aagtcaagga cctgaaaacc
     5521 tatttctatt ccggcaaaca cgcgctcaag gccgtggacg gtgtgagctt ttccattcgg
     5581 gagggcgagg tgttcggcct tgtgggagag agcggcagcg gcaagagtat cacctgcaaa
     5641 tccctcatcg ggctcattca ccgcccgggg cgcattgcgg gcggcagcat ccgctaccgc
     5701 gggcaggagc ttgtgggcct ctccgaaaag gcgctcaccg ccgtgcgcgg caaagagatc
     5761 ggcatgatct tccaggagcc gatggtggcg ctgaacccgg tgctgcgcat cggccagcag
     5821 ataacggaat ccttcaagga ccccgggctt tccggcgagg aaaagcacgc ccgtgccgtg
     5881 gagctgctgc ggcgcgtggg cattcccaac ccggagcagc gcatgcggga atatccgcac
     5941 cagttctccg gcggaatgcg ccagcgcgtg gtcattgcca tcgcactggc ctccaacccc
     6001 aagctgctgc tggcggacga accgaccacg gcgctggatg tgaccatcca ggcccagatc
     6061 ctgcagctgc tgcgggagct gaaggacgaa tacggcatgg ccatggtgct tgtgacgcac
     6121 aacctgggcg tggccgcgca gatctgcgac cgcatcgcgg tgatgtacgg cggtcatatc
     6181 atggagcttg gccccacgcg ggaactgttc gcacacccgc agaacccgta tacccgcggc
     6241 ctgttggcct cgctgcccgt gaataaacgc aagggcgagc cgctctccgc cataccgggc
     6301 acgccgccga atctgggcga gatgcccgtg ggctgcccgt ttgccccacg gtgcgcgcgg
     6361 gcgcaggacg tgtgcttcag ccagcctctc gccgagcggg aggtcggccc cggccnnnnn
     6421 nnnnngcccc ggccacacct gccgctgcca tttcgcggac ggcccctgcc gagaggagga
     6481 tgactgtgga aaaccgcaat aacgcacaag agcccctgct gcaggtgcgc gggctgagcc
     6541 tgcgctttcc tgtcaagcag ggcctgctgg acatcctgat gcgccgcccc cagcaggcgg
     6601 tgacggccgt ggacgatgtg gaccttgcta tctacccggg tgaatgcctg ggccttgtgg
     6661 gcgaaagcgg ctgcggcaaa agcacgctgg ccaaaaccat catccgcatc tacgacccgg
     6721 acgcgggcac cgtactgctg gacggcgtgg attttaccgc gctgaagggc aaacgcctgc
     6781 aggccgcgcg ccgcagcgtg cagatggtat ttcaggaccc atactcctcg ctgaacccgc
     6841 gcatgagcgt gggcgccatg ctcaaagaag tattgcaggt gcatcatgtg tgcccgaagg
     6901 acgaaatgga cgcccgtgtg gcggctctgc tggagatggt gggccttgcg ccggacgccg
     6961 cacagcgcag ccccggtgaa ttttccggcg gccagcgcca gcgcatcggc atcgcccgca
     7021 cgctggccct gcagcccaag ctgctcatcg cggacgaggc ggtctccgcc ctggacgtct
     7081 ccgtgcaggc acagatcatc aacctgttgg aggagctgca gaaaagcctg catctgacga
     7141 tgctgttcat ctcccatgat ctttccgtgg tgcgctgcat gacagaccgc gctgtggtga
     7201 tgtacctggg caagatcatg gaaatgggcc ccacggaaca cctgttcggc gcgcccgccc
     7261 acccctacac gcgcaacctc atcgacgccg tgcccgagcc ggaccccgac tgtccgccca
     7321 aagagagcac gatgatcggc gagccgccca gccccatgga tatccccggc ggatgccggt
     7381 tccatccgcg ctgtccctat gcaaaaaacg tgtgccgccg cgctcagccc gaactcacgg
     7441 acctgggcgg cggacatttt gcggcctgcc atttccccta tgcgtgcgcc ggggcgccgc
     7501 agacggaagc tgaacaataa gcccgccccg cggcgcgccg cggcccggac aatgaatatg
     7561 ggaaaggaac atctattatg aaaatcaccg gcgtaaaaat cgaaaagctg ctgctggagc
     7621 tgaccagccc gttccgcgtg gcctttggca ccatcacgca ctccgaaaac gtaatgatca
     7681 aggtcaccac agacgaaggc gttaccggct acggcgaggc cgcgccgctg gcgttcgtca
     7741 cagccgaaac cacggattcc gtccttgcgg cgctggagct gttccgcacc gggctgatcg
     7801 gtatggaccc gctggacatc gaagccgtgc atgccatgat ggacggcctg atgggcggca
     7861 actcctcggc caaatgcgcg gtggaccttg ccatgtacga catccgcggc aaagccgcgg
     7921 gcatgcccgt gtacaagctg ctgggcggct attccaacac cgtgctcaac gatatcaccg
     7981 tcagcatcat ggagccgcag gccatggcgg acaaggcgct gagctatgtg cgcgacaagg
     8041 gctaccgcgt gctgaaggtg aaggtgggcg cggacgtgaa cgacgacatc cgcgcgctga
     8101 cgctcatccg cgagactgtc ggcccggacg tccgcctgcg cgtggacgcg aaccagggct
     8161 acgacgtacc cacggcgctc aaggcgttga acgcctttgc cgcgctgggc gtggaatcgg
     8221 tggagcagtg cctgcccgca tggaacatgg agggcgcggc ctacctgcgc cggaaagcgc
     8281 ccgccatcca tctgatgctg gacgaatcga tccacggccc ggtggacgca gcccgcgcct
     8341 gccgcatgga cgcggcggat atcctgaaca tcaagctgat gaaatgcggc ggcctgtatc
     8401 cggccgagca gatcaatgcc atcgcccagg ctaacggcct cacctgcatg gtgggctgta
     8461 tgatggagac aaaactttcc atcactgccg gcctgagctt tgtggcggca aagaaaaatg
     8521 taatggacgc agacctggac agctttatgg aatatgtggg cggcgagacc cacttccccg
     8581 gcggcttcac ccacaagggt agcgattttg tgctgtgcga cgcgccgggt ttcgggctgg
     8641 acatcgactt ttgatttacc ggaaaaggcc gtgtgcgggc gctccggcac ccttgtgccc
     8701 gggcggacgc ccggcctttt cccattttac ggagggatga gcatatggag acatggtcgt
     8761 tttattccgc gggcatccgc atcgcccgct tttggcccgc cgccggcacg ttggagtggg
     8821 cgtgcctgga cgacggggcg ggcgttttgg gcgcgcgcgg tgcaatgaac gaggagaacg
     8881 ccgaatggtt tgccaggcgg taccagtttt cgcccgcggc attttttgaa gggctgtccg
     8941 catggaacga agcgttcgcg ggaggcggct cgcccgtcag ctgcggcggc tcgcgctacg
     9001 accgccgtcc ggatggattt tacgcccagc gcgaggcgcg tttccccaag gatattttgt
     9061 tcgcggacgg cgctgttcgc gctcagcttt gttcaaacgg caacggaacc acggtgctgg
     9121 tgcaggacgg ctgggaggca cgcacgccca cgggcgcgtg gcttgcctat gcgcctgcgg
     9181 agccggcatg cccggtgcgc gcgctgggca cctttatggt gcccgcgcgg gatggggtaa
     9241 ggcttgccac ggatgtctat gtgcccggca atgcccaggg ccccgtgcct gtgatgctgg
     9301 tgcgcacgcc atacgacaaa acagctgctc cgtggaatta tttcaacttc gtgcgccgcg
     9361 gctatgcgct cgccattcag gacgtgcgcg gccgcagcgc cagcgagggc gattttctgc
     9421 cctgctacca tgaggtggag gacggagacg acacgctgaa ctggctcgcc gggcaggaat
     9481 ggagcaacgg ccgcgtgggc atgatcggcg ggtcttacct gggctatgtg caatggtgtg
     9541 cagcggccag cggcaacccg catttgcagg cgctcgtcag catggtcacg gcgggcagcg
     9601 cctttgccga cattccccgc cggggcggct gctttgaatc cggcatgatg gcctgggcct
     9661 ttgcggtgtc caaccatcag atggacgccg cccgcatggt gcgggacgac tgggacgacg
     9721 tgctgcagat ccgcccgctg gaaagcatgg cccgggaggc catcggcgag gacatccact
     9781 ttctcaacac ctggttcgaa cacccggaca acgacgaatt gtggcaaatg tccgactggc
     9841 aggcccgcag caagggcgcg cgcatccccg cgctgatctt ctccggctgg ttcgacgacg
     9901 acggcatggg caccacccag gcgctggagc tggtccacga ctggcccgcc gttcagcgta
     9961 aggccgtcct tggcccgtgg atccacagct ttaacagccg ctacgacatc cacggcgttc
    10021 ccatgggcct gcgggccatc cgctacgacg tggacctgct gtacttccag tgggtggacc
    10081 atttcctgcg ggatgtggaa aacggtgtgg agaaaggagc gcccgtggaa tacttcacca
    10141 tcggcgaaga caagtggaaa accgctgaaa actggcctgt cgcggcggcc cgcccccaga
    10201 cgctgtacct ggacggggag gccggcgcgg ccgtggaaaa ccacggcctg ctggcgctgt
    10261 ccgtgcctgg ggcggacgcc tccgacacct accgctatga cccggacgac ccggccgtca
    10321 acatcattga catgtcggaa aatgaaatcg aggtgccgga ggattacgcg gccgaggagc
    10381 tgcgcccgga cgtattgtgc tataccacgc cccctctggc cacggatgcc gtcatcaccg
    10441 gggactgcag cgtggagctg ttcatttcct ctgacgccga agacacggat ttcatcgtcc
    10501 gcgtcaccga ggccacgccg gacggaaagt ccatcaagct ggcggacggc gtgctgtgcg
    10561 ccaaataccg ggaaggcttt gaggctcccc ggtatctgca gccgggcgcc gtatatccca
    10621 tccatatccg caccacaaaa ttctccaagc gcttcgagaa gggcagccgc ctgcgggtga
    10681 ccgtcacctc cagcgccaaa aatttcctgt ttcccaacag caacacgcgg gcgggcttca
    10741 acagtgtgga gaccgtggtc gcccacaaca ccatccatca cggcccggcg cacccctccg
    10801 ccatcacgct gaacgtagaa aaggcattgg aattttaagc cccgcagctt ttttgctcat
    10861 atttccattt ttcgacagga ttctccatgg tttgcccggg cagagtgcgt tataataaga
    10921 atgctgccgc gttcccactt cgcggcggcg ggccgcggcc gttgctttga atggccgcgg
    10981 cccatccatt tcatgcggcc ggcagacgca ccgcccgtcc cccgagccgc cgcacagcct
    11041 tttctactac ttttgtctaa aaaagccatg ctttacgcac gctttacgaa caaaacgaca
    11101 aaaatataaa aaatagcaaa acatgtccga ttttggcagg cttttgtgat acaatatcgg
    11161 tgtagtatgc ccggccccgc ggagccgggc caaacccgct ttgcggcatc ataaaattag
    11221 gagtgtgctt tccccatggt gcaagccaga accagtgcga aggaattttt caacgccgat
    11281 gaacgcctcg ccgacctcgg cctgattgcc gtgcccggcg ccgaggagct caccgccaaa
    11341 atcgaccgtc acctgctccg ctgggcgcag gaggccggga tggaccagga cacgtttatc
    11401 atcgagagcg aatgcccccg cttttcctcc ggcgacggca agggcctcat ccgctccacc
    11461 gtgcgcggcg acgatatcta tttcgtcgta gacgtgggca attacaacca gaaatacaag
    11521 attttcggcc atgagaactt tatgtccccg gacgaccatt tccagaacct gaaacgtctg
    11581 atccaggcca ccagcggcaa agccaagcgc atgagcgtca ttatgccgct gctgtacggc
    11641 agccgccagc accgccgcgc ctaccgggaa agcctggact gcgccgtcgc gctgcaggag
    11701 ctgcaggcca tgggcatctc caacatcgtc acctttgacg cccacgaccc ccgcatgcag
    11761 aacgccgtgc ctctgatggg ctttgacaac gtgatgccta cctatcaggt gctgaaagcc
    11821 atgctccgcg ccgttcccga cctccagctg gaccgcgacc atttcatggt catcagcccc
    11881 gacgagggtg cgatgaaccg taacatgtat tacgcctccg tgctgggggt gaatctgggc
    11941 atgttctaca agcgccgcga ttattcccag gtggtaaacg gccgcaaccc catcgttgcc
    12001 cacgaatacc tgggtgaaag cgtagagggc aaggacgtgt ttgtggcgga cgatatcatc
    12061 tcctccggcg aaagcatgct ggacattgcc tacgcgctga aaaagcaggg cgcaaagcgc
    12121 atttttgcca acgccaccta cgccattttc acaaacggcc tggattcctt tgacaaagcc
    12181 tatgccgacg gcatgatcac gggcgtgttc ggctccaacc ttacctaccg cactccggag
    12241 ctgctgagcc gcccctggtt ccatgaggtg gacgtatcca agtatatcgc ctatttcatc
    12301 gccgcgctga atcacgacct ttccgtcagc gccatcatcg atccccacga aaagatcaag
    12361 gctctgctcg aacgcaccgg acaggcccgc tgagcatttt ccttcgtttt taccggaagg
    12421 cccgctgttt ttacagctgg cctcttgttt tccgccgttt ttcggcgctc gaccagcggt
    12481 cggttgtcaa aagcgggctt tggggataca atcaggacaa aataaaaaca gcgcggcaaa
    12541 gccgccgcgc agggaggaat cgaatggacg ccagatatac gggagagcag atcgcggccg
    12601 cgcgccgcgc caaagggctg acgcaaaagc agcttgcgga cgccttggga gtaacggata
    12661 aggccgtcag caaatgggag cgcggactga attatccgga tattgtattg ttccagccca
    12721 tctccgagtt gcttgggctt ccgcttttgc agcttttctg caaggacggg ctttccgccg
    12781 agcaggccgt ggcggcggcg gcggcgcttt ccgcacagga aaagcaggcc gtcaggcgcg
    12841 acatgaaatg gcacgcggta ttccgcctgg cggccgccct gacgctgtcc gctgcgctgg
    12901 tctatgtttc ccgcgtgctg gatctgcac
//
LOCUS       DAWBSB010000034        12642 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1188_length_12642_cov_3.473028, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000034 DAWBSB010000000
VERSION     DAWBSB010000034.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 12642)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12642)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12642
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1188_length_12642_cov_3.473028"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1926..1935
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    12202..12211
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2511 a         3610 c         4026 g         2475 t
ORIGIN      
        1 gaaaaataaa aaaaacccgc cgtccggaag ctgcaacagc cgggcggcga gaattttttg
       61 aggctaaaaa tatttctcaa acacttctcc caggcgtaaa atagttttct ggtgcttcgc
      121 atcctgtgcg tcggtttttg gattccagaa ggcagctggg agtcggtgga ttgcaatttt
      181 ttcctatatt tcttcaagcg aacattcggt gcggtctttt tccgctcttg gtatactatg
      241 aatgtggaaa tatggtgcat tttgtcctgc gttttcatcg ctggcgtttg acgaatttat
      301 gcttttacaa ctgtattttt gtcaaatgat ttaaagtttt ttacggtatc tataaccttg
      361 tataatgcac gggtgtacct ttgccgtaat ccaaaatatt tttgcagtgc tccttgggac
      421 aaccaataga tacccgcctg catctttggc agcatcccaa aaagtaaaag tatcatttta
      481 ccgttcttgc ccgacagagc cacctcgctg aaaattggct ctttacagcg ccgcagcgtg
      541 ctgcgcgcgg cgaaaatagc tgcgggccat ccacagcgcg gcaaggaagc ccagagcgtc
      601 ggccagcggc attgcggcca tcaggccgtt catgctccag gcgcggggca gggccagcag
      661 cagcgggatg tagatgcaca cctgtttgcc aagaaccagc gcgctgctgc gcgcgctttc
      721 ccgtatggcc tggaacagcg tgcccgtgca gttgatgaac gtggtgaacg gcagtgcgat
      781 gctggtggcg cgcagcacgg ccgcaccgtt ttccacaagg ccgccgtcgc tggggctgag
      841 catgcgcacg atccatggcg cggctgcggc aatgatcacg gccagtgccg tcagagcgga
      901 catactggcg gcgcagcaga agcggaacgc gcttttcagc cgcgcgtggt ctttgctgcc
      961 gtgggcgtag ccggccacgg gctgaagccc ctgcacaaag ccgatggaca cggagagcgc
     1021 cagccccgag acgcgggatg cgatgctgaa cgcggcgatg gcagcgtctc caaagccgcc
     1081 cgctgcgcgg ttgagcacga tggtggccag cgcgttgccg cagtgacggc acaggctggg
     1141 aaagcctgtg gtgaaaatca gcttcaccat ccgcggggtg cgggctacct gccgcaccga
     1201 aatgcgcacg gatgttttgc cgcgcacata gaaccagagc agcgcggccc acaccatcac
     1261 ctggctgagc gccgtggccg cgcccgcacc ccggatgccc atgcccaacg tgtaaatgaa
     1321 cagagggtcc agcacaatgt tcaaaagccc gcctgcaagc tgtccggcca tgctttcctt
     1381 gctttttccc tcggcgcgca ggcactgcgc cagcgccagg ctggagcatt ggaaaggcgc
     1441 ggccagcagg atgggaacgg cgtaggcctc ggcttcgggc aggatggtat cggtgctgcc
     1501 cagcaggcgc atcagcggct ccagcgccag cagcccgccc gcagcgatca cgccgcccag
     1561 ccccaaagca agaaaaaagc ccgtgctggc ggcgcagtgc gccttttcct tttcccgtgc
     1621 gcccagatac aggctgacaa ggctgctgcc gcctgtaccc atccagaagc ccagtgcaga
     1681 gagcaccgag gatatggaaa acacaacgcc caccgctgct acggcggccg tgcccagctg
     1741 cgccacaaag aacgtgtcgg ccatgttgta gaccgaagtg atgatgcagg tgatgatgct
     1801 ggggacggag agagagagga taagccccgg caccggtgcg gtcgtcatac gcaggtgctg
     1861 tgcatcctgc tgttccgaag tgctgttcat gccgcaaaac tccttacggc aggcctacgt
     1921 cctatnnnnn nnnnnggacg taggcctgcc gcttattttt taccgattca ggatcgccat
     1981 gaattcatcg ccggtgatgg tctctttttc cagcagatat ttggcaagtt cgtgcagctt
     2041 atccatgttg tctttcaaaa tgccgatggc cttttcgtgg gctgtgcgga tgatctccac
     2101 cacctctttg tcgatcttgg tggcggtttc ggcactgcac gccagggctg cgtcgccgcc
     2161 cagatactgg ttgctcacgg tctccagcgc cgtcatatcg aatgtttcgc tcatgcccag
     2221 gcgcgttacc atgctgcggg ccagcttggt ggcctgctcg atgtcgttgg acgcgccgct
     2281 ggtgacgctg ccgaagatca gctcctcggc gctgcgcccg cccgtcagcg tggcgatctt
     2341 cgccagcgcc tcttccttgg agagcagcac cgtgtcggcc tccggcgtct gcatcgtata
     2401 gcccagcgca cccgaggtgc gcggcacgat ggtgatcttg gtcacgggcg cggaattgga
     2461 ctgcagcgcg gcaaccagag catggccgat ttcgtgatag gcgacgatct ctttttcatg
     2521 gggcgaaagc actgcgttct tgcgctggta gcccgccacc accacgtcta cggattccgt
     2581 caggtcttcc tgtgtgacga tgcggcggtt gcacttgacg gcgcgcagcg cggcttcgtt
     2641 gatgaggttg gcaagctctg cgccggacgc gccggaggtg gcccgcgcca cggccttata
     2701 atcaatgtcg tcggccatca tcacgtcctt ggcatggaca cgcagaatgg cctcgcggcc
     2761 ctgcaggtcc ggcagttcga ccggcacacg gcggtcgaaa cggccggggc gcagaagcgc
     2821 tttgtccagc gtttcgggac ggttggttgc cgccagaatg acgacgccgg acgagccgtc
     2881 gaagccgtcc atctcgctga gcagctggtt gagcgtctgc tcgcgttcgt cgttgcccgc
     2941 gatgccgctg ccgtcacgct ttttgccgat ggtgtcgatc tcgtcgatga acacgatgca
     3001 gggggccttt tctttcgcct gtttgaacag gtcgcgcaca cgggccgcgc cctgtcccac
     3061 gaacatctcc acaaactcgc tgcccgcgat ggagaaaaac ggcacattgg cctcgccggc
     3121 cacagccttg gccagcagcg tcttgccggt gcccggaggg ccgacaagca gaacgccctt
     3181 gggcattacg gcgcccacgc tgcggtattt atccggattg tgcaggaaat ccacgatctc
     3241 ctccagcgct tccttggcct cctcctgccc ggccacgtcg gcaaaggtct tgcccgtctg
     3301 agcggccacg tacatcttgg cgttgctctt gcccatgccg aaggacatca tattgctgcc
     3361 gtcgcccatg cgcttttgca tcatacgcca caacagcacc atgggaagga tcaggatgag
     3421 cgtggggaag atccaggtga acaggaaatt catcagggtg ctgttttcct gcggagccac
     3481 ggcggaaaag gtgacgccgc tctcggacag gcgcttttgc aggtccgcgt ccgggaacac
     3541 gcccgtggag tagatccgtt ccgccccgtt ttcatctgca gctttgaaat aaagctcacg
     3601 ctcgttttcc tgcagctcca ccacggtcac ctggccgccc gaaagcatgt ccaggaactt
     3661 gtcgtagctt acttccgtca cgcgcggggt catcaggctg gggaacacaa acgcgttcag
     3721 cagcatcagg atgagcatcg cgatcatcgc atagtaaaag aatggctttt tattgggttt
     3781 gggttgctcg ttcatatgct tcaactcctc actgtatctt ctattgtaac gccggaatat
     3841 gacaaaattc aagcgcgttt gtaaacgtgc tgtgaatacc ggcattttcg ttttgttttt
     3901 ttgccggaaa catgctatac tggcctcaaa cggcggcgtg tgccgccttg cggagaagca
     3961 cggagggagg cgcggcccat gacggcggcc cggcgcagag atgagatatt gcaggcattg
     4021 gccggggcgt ccggcccggt gagcgcggcc gcgctggcgg cgcgccttgg cgtgagccgg
     4081 caggtcgtgg tgggggatgt ggcgctgctg cgggcggcgg gctcgcccat tgtagctacg
     4141 ccgcgcggct atgtgctggg cggcggacag gagggcggcg gcgtgcgctg caccgtggca
     4201 tgccgccacg gcctggacgg cctgctggac gagctgtata ccgtggcgga ctgcgggtgc
     4261 ggcgtgctgg atgtgaccgt ggagcacccg gtgtacggcc agctttcggg tcagctgcag
     4321 gtattcagcc gttatgatgc ggacgttttt tgggatgccc tgcagaaaaa cggcgcgcag
     4381 cccctgtgca gcctgacggg agacatccat ctgcacacgc tcgtgtgccc ggacgaggcg
     4441 cgcaaagcgc gggtgctggc cgcactggag gagaagggat atctgatggg cctggaaaaa
     4501 tgacgaggct tgctgcgccg ggcggtagta tgcccggcgc gcctgcggtt ttgcgggagc
     4561 cctcggacgg gaggacgcga tgaggacttt gtatgtatcc gatctggacg gtaccctgct
     4621 gcgcagcgac gaaaggacat ccgggtatac caaccggacg gtcaaccggc tggtggggga
     4681 aggcatgctg ttttcctatg ccacggcgcg gtcgttccag acggcgcgga aggtgacggc
     4741 cgggctggaa gctgaaatac cgctgatcgt ttacaacggc gcagcagtgg cggacaaccg
     4801 ggacggctcg tttttacaga tgaatttttt cggcggagaa gcggcggaga tcctggacga
     4861 actggaggca tgcggcgtgt tccccatcgt ctatgcggtg gccgggggca gagagatgtt
     4921 ttcctatctg cctgcccggt gtacgcgggg aatgcgggcg ttccttgaga cgcggaaaaa
     4981 cgacagaagg gcgaaccccg tcgcaacgcg gcgcacactg cgtgcggggg atatttttta
     5041 cttcacctgt attgacgaga gggaaaagct gctgcctctt tatgaaaaat accgggagca
     5101 atatcattgt gtgttccagg tggatctgta cacccgggag cagtggctgg agatcatgcc
     5161 gaaagcggcc tccaaggcca gtgcggccct gtggctgaaa agcagcctcg gatgcgggcg
     5221 gctggtcgtg ttcggggacg gagaaaatga tgtggacctg tttgaggccg ccgacgaggc
     5281 atatgctgtg gcgaatgccg cagatgtgct gaaggccgtc gccacgggcg tcatcggcgc
     5341 aaacgacgag gacggcgtcg cccggtggtt agacgcgcac gcgcggtgtg ccgcgcagac
     5401 gcagggcggc gtatagggcc gccccgccgc aggaacggca aggcgtgttg cccaaacaca
     5461 gtgtttccgg cggcattatt tttggtggaa aaaggggaat ttgccgcctg cggttgacgc
     5521 aggggcccca agcgcgtata gtatagaaaa aatatgtgtc aagacacgtg tcgtgcatgg
     5581 tgtctgcaca gtggatgttg gggaggaacc tgtttatgtc cgcagccaaa aatactgtac
     5641 gcattcagag aatgtgcgtg gccgcgctgc tgtgcgccat cgccatattg atcccgatga
     5701 tctcgcctgt aaagatacag ctggggccca tgagtttcac gctgggcagc catgtggcga
     5761 ttttcattgc catgttcatc tcgcctgcgg tggcgctcac ggtggagctg ggcgcgacac
     5821 tgggcttcct gctggcgggc tttccgcccg tggtggtgct gcgagcgctc agccaggtgg
     5881 tgtttgtggc cataggcgcg ttcctgctgg cgaaaaaacc cgctgtgatg aaaaacggcc
     5941 tgagtatttt cctgttcggg ctggtgatgg gcgtgatcca tggcgttctg gaagccgttg
     6001 tggtgacggc gttttggttt tcgggcatga cgatggaggg gacgttcgtc tccacggtgc
     6061 tgggccttgt gggcgtgggc acagtggtgc acagcatggt ggactattac ctggcgctgc
     6121 tgatctggca gccggtgtcc cgcgcggcaa agctgcccgc gagcttcaca ttttcaaaga
     6181 gctgagcatt ccggaggggt tttgaataaa gcggcggggc gcgttgggcg ctctgccgct
     6241 tctttgcgtt cgaatgcttt tgtgttacaa tgaaccgaaa gagccgtgca ataatccgcg
     6301 cggctcattc gtatgaaaaa tgtgagggtc ctttggtgaa agggggaagg ggtatgagcg
     6361 acgctgccgt atgcgccggc gccgaggaag ttttggagcg ctacgccgcc acggtatttg
     6421 ccgcggcgta tgccatggtg aaaaacaaag aggacgcgga ggacatcgcc caggaggtgt
     6481 tcatcagcta tgtaaaggca aaacctgcgt ttgagagcgc cgagcatgaa aaggcctggc
     6541 tgctgcgggt gaccatcaac cgctgcaaga gtttttttcg cagtgcctgg cagcgcaaaa
     6601 cgaccggcct ggaggatgat ttcccggaca cgcccttcac ccccgacgag accgccgtag
     6661 tggaggcggt gaaccgcctg ccgaaaaaat acaggcaggt catctactta tattatattg
     6721 agggttatgc caccggtgag atcgcggcca ttctggaaat gccgcagaac acggtgctct
     6781 cgcagcttgc gcgggcgcgc aaattattga aaactgcttt gaagggggag tttgacgatg
     6841 tataaagacg attatatctc cgcgttcggc aagctggccc ccagcgagga atggatgacc
     6901 gacacgctgg ccaaaatgcg cgccgccgag gcggagcagg aagcgccggg cgcggccggg
     6961 cggccggcac tgcgcgtttc gggcaaaaaa catccgcgcc tgcgccgcgc ggcactgccc
     7021 gtcgcggcgg cggtggcgct gctggcggtg ccggcggcct ggttctgggg cgtggcgatg
     7081 cgcccggcgg gcagcgccgc ccccgcgcag tacagcctgc agagcgccgg gggcgcggcg
     7141 gacgagggcc aggagacgga acaggcgccc cgcgtggccg cagcgcagga cacagcgggc
     7201 acggcaagct ctgcggcggc agcgccggag acaacggagg acgcgcaggc cggcgcgcgc
     7261 aaggagggca ggctgctgtt cgccgcggaa ggcggcggta tgggcatggg agatattacc
     7321 gtccggacgg atgcggacct ggcgggcgcg aaccccacct atgacctgcc tgccggggaa
     7381 ctgcccacag agctgcctgt gtatgcggtg ccggacggag aagcagagat gcgcgcggcg
     7441 ctggaggaca cggcggccaa actgggcggc acgctggagg cgttttcata cgatacctcc
     7501 ggcccgccgg acacggcgta ttacagcccc tatgccagcg gcaaggcgga cggcgtaagc
     7561 tattccctaa acgggcagga agtacatttt taccggtatg aacaggggga taacttattg
     7621 gctgcgccga aagggctgtc cggcgaagcg ctgtaccgct atttctacga tcatttcggc
     7681 gcgaagtttg tgacgctgga aaatcctgcg tttgaatcca cgggcgatta taatatctac
     7741 aaagagtaca gtacggccat cagtgcgttt tatgaagcgg ggagcgcttc ggacacgctg
     7801 gcgcaaaagc tgtacaatta cagcttccgg cgcatccagc tttccgcgcc ggaaggggac
     7861 ctgaccgctg tgacgttccc gctggaaccg caggtgctgg gcacctatgc cctgcgcacg
     7921 ctggacgacg caaagggcgc gctgatggcc ggtgaggcct ggatcggcgg cacgcagggc
     7981 ggttacgacg gcggtgcggt gaatatcctg cattgggaga tcacctatta ccggagccgc
     8041 ctgatggata ccatccagcc cgtttactgc tttttgatcg acagcccgga cggcgaagcg
     8101 cctttttttg acgacggaga cccggaaggc tacaagagcg tgacctacgc ctatgtgccg
     8161 gccctgctgc cggagcagct gagcgaaaat acgtttgtcc aagttttcaa ttgacgatcg
     8221 ctgtgaaaaa acgcaaaaac acgaaggcgg cggaaagaag cttacagggg ctgcatagga
     8281 cgtccgctat tcttccatct gcttgcccag aaatgcggcg gctaccttta ccagcagcac
     8341 catggcttcg tcgggcatgc tgcccgtcag cgccacgctg ccgatgaggt ctccgttgca
     8401 caaaatgggc atcagggctg tggcgcttgt gtccacgccc tcgcacaggg gcagggcgct
     8461 ggcggcgtcc gccgccgtga acagacggcg ctgctctacc gcgcgggtat aaacgggagc
     8521 gagagatctt tcaacggcgt cccgcgctgc tccggccgca gccaccacgc ggtcccggtc
     8581 cgcaatggcg acggacgcgc ccgccgcttt gtagagcacc tccgcatagc ttgcggcaaa
     8641 ggccgaaagt tccccgatgg ggctgtattt cttgaagatc acctcgcccg cgccggagac
     8701 gaagatctcc agcgggtcgc ccacgcggat gagctgggtt cggcgtattt ccttggggat
     8761 gaccacacgc ccaaggtcgt cgatacggcg cacgatgccg gttgctttca taattgctgc
     8821 ctcccaattt ctgatgcatg atactattat ctgttaatac agggaaaagt atacaggcat
     8881 tcggaaaaag aaaaacacaa agcgaaagcg cgcccagccg gctgttcagc cgtgcgggac
     8941 gcgctttcgt tttaagaaaa aggaaaagga gaaggtgcgg atcagcagcg gccgtacagc
     9001 ttcgtcaaag ccagaatatc ggccgccaga gcgtccgtca gcgcggcggg gtccatgcgc
     9061 cacagccagt tgccgcccag cgtggagggc gtgttgatgc gcgcctcggc gccaaggccc
     9121 agccagtccg gcatgggaat aactgcggtg tccgaaacgc ttaccagcgc tgcgcggata
     9181 aaacgccgcg tgaaatcggc gtcgccctcc acatccagat aacgcctggc gaacgccacg
     9241 tccccggccg gagccgaaag ctcccagtcc gccgtggtgg tgttgtcgtg ggtgcctgtg
     9301 tacacaacag aattctgtgt gtagttgtgg ggcagatagt cgctttcctc ccgggaatcg
     9361 aaggcgaact gcagcacctt cataccggga aagccgctgg cgcgcagcag tgcctttaca
     9421 tcgtccgtca gatagcccag atcctcggca atgatgcgca cgtccggcag ctttcggcgt
     9481 atgacgtcga tgaacgccgt gccgggcccc ttgcgccaca cgccgttcac tgcggttttg
     9541 tcccgggcgg gaatggcgta atagctttca aagccgcgaa agtggtcgat acggaccaca
     9601 tcgtatacgg cgcttgcatg gcgcagccgt cgtatccacc aggcgcaccc ggtctttaag
     9661 tgtacatccc agttgtacag cgggttgccc caaagctgcc cgtccgccgc gaaagcgtcc
     9721 ggcgggcagc ccgccacctc cgtcagcgcg ccgtccgcgt ctacctggaa cagcgaaggg
     9781 tcggcccaga ggtcggagga atccggcgaa acgtagatag gaatgtcacc gatgaactcc
     9841 acgccctttg cgttggcgta ggccttcaga gcggcccact gctcgtaaaa caggtattgc
     9901 accacctgcc agaagcggac ctgccctgcc agacgcgccc gtgctgcggc cagcgccgcg
     9961 ggcctgcgtg cgcgtacgtc tttgggccac agcgaaaagg cgcgcatgcc gttttccgcc
    10021 ttcaaagcca tgaacagcgc gtaatcgtcc agccagaacg cgttggcggc gcagaaatcg
    10081 cggaatgcgt cctccccggc atcaaagcgg cgcacggcca gctccagcac ggcaaagcgc
    10141 gtgcggaaca gggcgccgta gtcgatgcct tcgggcgctt cagggccgaa atcaaagcgt
    10201 gcgatctcgt cgtgcgtcag caggccgcgc tgctccagcg catcgaggtc gatgaaatag
    10261 gggttggccg caaaggccga aaagctctgg tacgggctgt cgccgtaacc cgtgggcccg
    10321 atgggcagga tctgccagta ccgctgcccg gccgccgcca gaaaatccac aaaacggtag
    10381 gcttccgccc ccaggctgcc gatgccgtag ggcgagggca gagaggagac aggcatcagg
    10441 atgccgctgg tgcgcatcag cctttcaccg ccccggccac gacgccctca atgatatact
    10501 tctggcacgc caggtacaga atgatgatgg gcagcaccgt gagcatgatg caggccatca
    10561 tggggcccat ctccacgcgg ccgtagctgc cgcggaagta ctggatgagc atggggatgg
    10621 tgcggtattt tttgatgtcc agcaccagcg tgggcagcag atagtcgttc cacacccaca
    10681 tggcctccag aatggcgacg gagatcatgg tgggcttgaa aatgggaaat acgatgcgga
    10741 aatacgtttg gatggggttg cagccgtcga tcatggcagc ctcttcgatc tccagcggga
    10801 tgcttttgac aaaaccgcag aacatgaaca ccgccagccc cgcgccgaag cccaggtaga
    10861 tgatggcaat gttggagggc ttgttcaggc ccagactgtc cgccgtctgg gaaagtgtaa
    10921 acatcaccat ctggaacggc accaccatgc tgaacacaca cagatagtac atggccttgc
    10981 tcatcagaga attgacgcgg gtgatgtacc acgcgcacat ggaacagcac agcaggatga
    11041 gtgccacgct ggtgacggtg atgagcgtgc tgtaccagaa actctgtaaa aagcccttgg
    11101 aagaaatggc gttgacgtag tttgccgcgc cggcaaaggt atctgccgtg ggcggcagga
    11161 acgccgtaga agtactgatg gaggtctcct ttttaaagga gttcagcagt atcatgagga
    11221 ttgggtacac atacaggaca gatgctacgg agagcagcac cgtccagaac agattgaagc
    11281 ctttttttgc cttgaccatt actgctgcac ctccctggaa cgtgttgccc tgagctgaac
    11341 aagggctatg gctaccacga gcacgaagaa aatgacggcc tttgcctggc cgatgccctt
    11401 ccactgcggg ccggagcggg tatagaacgt gttatagatg ttcagggcca gcatctcgga
    11461 ggcgtgattg ggttcgccgc ctgtcagcga aaggttctgg tcgaacagct tgaatgagtt
    11521 cgtcagcgac aggaacaggc atatggtgac ggaggacatg accatcggca gcttgatgcg
    11581 caccagcgcc tgccaggggt tcgcgccgtc gatctgcgca gcctcgataa ggtccccggg
    11641 cacgttctga agcccggcga tataaatgat catcatataa cccacctgct gccagcacat
    11701 caggatgatg agcccccagt agccggccgc cgtgttgagc tgcagcagcg gctgtttgac
    11761 cagcgacagc acgcagttga tgaggatctg ccagatgtag cccagcacga tgccgccgat
    11821 gagattcggc ataaagaaca cggtgcggaa tacattggtg cccttgatgc cccgcgtgag
    11881 cagcagcgcg atggcaaagg ccagcacgtt gatgagaatg gtggacacgg ctgtgaagcc
    11941 cagtgtgaac cagaacgcgc tgcggaacgt ggcgtccgaa aacgctttga cataattgga
    12001 aacgcccacc caagaggcgt tgcccaccgt ggtgaatttg cagaaggaga gatagatgcc
    12061 ctctgcaaag gggacgatga acccgatgat gaacgcggcc agcgtcggca gcacaaacag
    12121 cgggaaatat tttttgagcg tcttttccat ggcggctgcg gtcccttcct ttcctttttc
    12181 ttttgcttac ttataaaatg gnnnnnnnnn ncacgaaagc ggagacaacg ccgttccagt
    12241 cgcccgtgcc ggcggcatac gctgtcagcg cagaaccaac gccgtttttc cattcctcgg
    12301 aaggcatggt ggtgaagttc cacgacaccg gggtcttgcc cgcggcggtc atctcggcgt
    12361 cctgctttac gaacaggttc ggggactcct gcgcggtttt gaacgggatg acaaagccca
    12421 tgtcgtcggc catggccttg gtgccctgct cggaggtgac gcaccagttg atgaagtcca
    12481 gcgtggcctg gatgtcgtcc tcggaggctt ccttgttcac gcaccagtag ttctcggtgc
    12541 cggtgcacag gccttggttg gcctcgtcgc ccacgccgat ataaatgggg atcatggcga
    12601 ggtcgtcatc cgtatacttg ccctcgcccg tcaggttgcc gt
//
LOCUS       DAWBSB010000035        12564 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1194_length_12564_cov_3.726117, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000035 DAWBSB010000000
VERSION     DAWBSB010000035.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 12564)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12564)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12564
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1194_length_12564_cov_3.726117"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2465 a         4136 c         3700 g         2263 t
ORIGIN      
        1 gaacacatcc atgccgcgct caaactttcc gctgcagatg cggatgaagg cgatgcggtc
       61 ccggtgggcc ttattcatat tggcctggat cttgaacacg aatgccgaaa aatcctcccg
      121 tctggggtcg atggggccgg cggaggaatc ccgcggcagg ggcgggggcg aaaggcgcag
      181 aaaatccgcc aggaacggct ccaccccaaa gttcgtgagc gccgagccaa agaacaccgg
      241 cgtcagcatg ccgtggcgca ccttgtccag attgaattca taggccgcgc cgtccaacag
      301 ctcgatgtcc tcgctgagag tgtcgtgcag cggcttcgtc agcagttcgt ccaggccggg
      361 gtcgcccagc tgcgcctcga tcttttcggc ctgctgctgg cccaccttgc ccgaatgctg
      421 gaaagcgagg atctcctttt tgttgcggtc gtacacgccc ttgaattcct tgcctgagcc
      481 gatgggccag ttcatgggat atgtttcgat ccccagcacc tgttcaatct cctccagaag
      541 gtcgaaggga ttctgcgcgt ccctgtccat cttgttgatg aaagtgaaaa tggggatgcc
      601 gcgcatggta cacaccttga acagcttttt ggtctggttc tccacgccct tggccgcgtc
      661 gatgaccatg accgcgctgt ccgccgccat cagcgtgcgg taggtatcct cggaaaagtc
      721 ctgatgtccc ggagtgtcca ggatgttgac gcaataaccg ttgtagcgga actgcagaac
      781 agagctggtc accgagatgc cgcgctgctt ttcgatctcc atccaatccg acacagcggc
      841 gcgcgcgccc tttttgcctt tcacgatgcc cgcctgctgg atggcgccgc cgtacaaaag
      901 cagcttttcc gtcagcgtcg tcttgcccgc gtccgggtgc gaaatgatcg cgaacgtgcg
      961 gcgcttatcg atctgttcga taaagttttc cacaaaaatc ctccaaaccc gatatccatg
     1021 ttgaaaatac aatttattgt atgatattcc gcgcaaaaaa acaaggcgga cgaccaaaaa
     1081 agccgcattt ttgcggcgct cgtgtcaaag aggtcacacg atgatgtttt tgatgagcag
     1141 cgacatggag ccgtccgggt tgcggatgaa ctccagggcg ctctcgtccc ggtacacatt
     1201 caccggtacc ttgatctcaa cgccgcccgc gctgcgcagg ctctgctttt ccatacggcg
     1261 caccgccggg gccgaaaggg gcagcggctc gtccagcgtg atatcatgct cggccagagc
     1321 tttggtgaaa gcctcccgcg cgtggggcat atcgccgtac agcttgtcgc agatcgcctg
     1381 cacgggaacg gggtccggct tgcccgccct ggatttttcc tccgcgcggg ccgcataaaa
     1441 ttcctcgcac acagccatgg cgagctccgg ctcgtccacg ccggtgttcc cgtagaactg
     1501 ctggtttacc tcacccgcaa ccgcctggat ggccgaaagc ttctcccgcg gggacaaccc
     1561 tgcccgacag cctaggattc ggctgcccag gtacgtttgt ttgcggccgt ctatctcgta
     1621 ctttttttcc agcaggcgca cggcgttagt gcgcaggtcc acaaaaaacg cctcgtccgc
     1681 cttaccggac gtgcccggca gcaccgtgcc ctggcgcact acctcattga cggcgggccc
     1741 gccgtccata cggcagtaat gcagccaccc gggcttgtaa ttcagcttca gcgcggccag
     1801 atattccgca ccgtcgatct ccagcagaag aaacgccgcg tctcccgcgg ggatggccgg
     1861 attttcctgc atcacacgga accagtctcc ggcaatgaca gccgtctttt tttcaaaatc
     1921 atcctcgatg ttccataaga gcggcaggaa gggcgaatcg tccgccagcg tgcaggcctt
     1981 ggcctcgtcg ctggcaaagc acttggctgc gtggccggcc agatattgca gcgcgtcctc
     2041 ggagggcggc aggatggcct cggataaaat gggcgcgtcc acggccgggt ccagcacgtg
     2101 gaggatggcc ttgatgagtt cgatgttcat agctctgttc ctttcctttt ggcaaccctt
     2161 ttattatact aaaaaatgcc gcgtatgaaa aggtgccttc gtgcaggaaa acatctctca
     2221 aggtatccgc catgggcttt ggataagccg tccggccgcg aagcgacggc ggcaaatctt
     2281 cgccgccagg gacatgcccc ggcaattttg gatgcccggt gcgcgaaaaa accccggcaa
     2341 tccaaagagg atcgccgggg ttttaaatac acctggcgcc aagccgtcgg gggcaaagcc
     2401 gataccttcc tgcaccagag gtacatacag tgacatttgg gtcaaaccac aggcggacgc
     2461 gtcacgcggg acctgttctt gaaccgacgt tcagccgtac agttcctcca agaccgttcc
     2521 gcggaattcc agaaatgcgg cggcctgggt gcgcgcgctt tcgtacgcgg cggaaaggcg
     2581 gcgctccacg acagccatgt cgtccgcgct ttccatgggc agcagcagcg catactggct
     2641 gcggctgtat tttgtaaagg tgtcgctttt gcgcagcgtg ccggcgatcg ccagtttcaa
     2701 atcgtccatc gcctcggcca gcgcttcccc ctcgggtgca cggccgccgt ccggcccctg
     2761 aacggtgatg agcatcagcg ccaccacctg ccgcgcgcgc tgcgcggtac gggccacgat
     2821 ggcgtacatg cgcttgaaca cctcatagtt gcagtaaaac gcaccgcggt cgctgcgctg
     2881 gctgcgcagc gcgtcggaca gctcgtcccg aatggccgca aaatcctgct cggtcttatt
     2941 cacagcctcg gctgccgcgg cgtaaatgga gccgatctcc gcgcacagcg ggcgtcccat
     3001 ctcctcttca aaatagcgcg ccgtcttgtt gtaggcgatc acaatggccc gctgcatgtg
     3061 cagcgcgtcc agcgcacgga acgtccacag cgcgatatcc tcgtccagcg ggtccgtcac
     3121 agatgcgcgg gaggcaacgt ccagcagctc gccgtaggcg ttctccccgg caaggcaggc
     3181 gcacagggcg cgggcgctgg caagataccg gcgcgtaaag ccccgtgccg cgcgcatcac
     3241 ccacacctca ccggccagct ggggcagcag ttctccccgg aacgctccct ccgctttgcg
     3301 ccaggcttcc atttttgctt cgctgccgcc ggtgcagcgc tcggcctttt cgcacagctg
     3361 ttcaaagcgc tcggtatcga cgctccactt gatatgcccg ttcaggcaat aaccgcccgc
     3421 acggaacagg atggggcttt cagcgggctt gaccggcccg gccagctccc gccgcagcgc
     3481 ataaacggta tttttcagca cgttggccgg gtccgccagc tcctcgccgc cccacagctc
     3541 ctgcagcagc accgaggacg gagtgacatg ctcccggttg agcgcaaggt aacagaacag
     3601 ctgccacggc ttcgtcagct tggctgtggc gtccaccacg cagcgcccgt cggcaacgac
     3661 ggaaaagtct cccagcattt ttatggcgat cttctggagc atcgggccac ctccggcaaa
     3721 tttctatcca ttctgatagt accgcatttt cgtcaaaagg tcaaacattc tatgaaattt
     3781 ttaccgcttt gacttaaaat acggcaaaac gccccggcgg ggctgcgcgg ggaagcatgc
     3841 tgctcctccg cgcgcccgtg ccggggcgcg ttttcggttc ggtctgcgcc gggcatcagc
     3901 cctccgtacg ctgggtgctg cttgcctgcg gcgtggattc ctccagcgtg acgttcgctg
     3961 taacgatctg cgtaccgcgc accacctgca cctcgatggt atcgcccacc gcgtgttcgc
     4021 cgatgatgcc cgtgatatcg ctggtggaat tgacagccgt gccgtcgatg ctcacaaagc
     4081 ggtcgccggg ctgcaggccg gccttgtcgg ccgctccgcc ctcattgacg ctctgcacat
     4141 acacgcccgc ctggtttaca ccatactgga acgctgtctg cgcgtcgttg atgctcagca
     4201 ccgtgacgcc catggccggg cggccggtaa catatccgtt gttgataagc tcctcggcca
     4261 cctgaatggc cgtgttgaca gggatcgcga agcccagccc ttcggcattc tggccgctgg
     4321 acttggcgtt gacgatgcca atcagctcgc cgcgctcgtt gaacaggccg ccgccggaat
     4381 tgccggggct gacagccgcg ttcgtctgca gcaggttcat ggtctgattc tccacggtca
     4441 cctcacggtc cagcgcggag atgatgccgt ccgtcacggt tccgcccaac tcgcccaacg
     4501 ggttgcccac tgccagagtg aattcgccta cctgcagact gtcggaatcg cctacgaccg
     4561 ccggtgtaag gcccgtggct tcaatcttca acacggcaat atccgttttg gaatccgcgc
     4621 ccacgaccgt agcgggatac tcggtgccgt cgctcgtgcg caccgtcacc tggctggcgc
     4681 cgctcaccac atggttgttc gtgatgatgt agccgtcctc ggtgatgatg acgccgctgc
     4741 ccgcaccgct ctggacgtac tgaccaaaga acgcgttggt ggtaacggcc tcggtggtca
     4801 cctccaccac gctgggccct accttgctgg cgatttctgt gacggaaagc gcgtcggctg
     4861 tgttggccgt gccgcccgcg gcgctgcctg tgccggacga ggccgcgggc gcctgataaa
     4921 tgacggggcc tgtggagccg cccttggcga aggtgtagcc cagatatccg ccgccgaagc
     4981 ccacggcgcc gcagaacacc acggcggccg ctcccgcggc gacgcgcttg ccccagccgc
     5041 gttttttctg tgccgcatgg ggcggcaccg gcgcgccgcc cacacccatt ccgggcattc
     5101 cggcggggcc gggcgcgccc tgcaagggcg attccgcctc cggtgcagtc gtgttctgtg
     5161 catttctgcc ggggttgtta ttattgtaaa gttcggaata gtcgaattcc caattgttgt
     5221 tcataagtca acatcctcct tctttcctga tgttgctttc agtataggcc atgggtgtga
     5281 aagaactatc atacgggtgt gaaaaaaagg tgaaaccatg ggcgcgcggc acaaaaaggc
     5341 cgcggcgtca cacggaacgc gccgcccgtt caccaggccc gcttttcgat gcaaacgggg
     5401 atgggcggac agcccggccg gccggaaaaa tcgcgcacag acacacggta tccttccggc
     5461 ggcagacccg tgagaaacgc cagcaccgca tccttttccg ccatgccctg cggcccgccg
     5521 taatagacgc ataccgtcat cacaccgccc ggccgcagca gttccagcgc cgtgcgcatg
     5581 gccggcacgc tcacgcccgg cgtggtgaac actccgtggt cgccgccggg cagatagccc
     5641 aggttgaata ccgccgcgtc cacactgccc ggagccgcat aggcagccag gttcgcgtgg
     5701 ctgtccagca ccgcgcggcc tacctgtccg cagccgttct cccgcaggcg ggcgttggtg
     5761 ttctccactg cctgcggctg gacgtcaaag gccagcacac gtcccgcagc gcccaccaga
     5821 cggcacagga acagtgtgtc atggccgttg cccgccgtcg cgtccagcgc gaaagcgccg
     5881 ggccgcacat gcgtccgcag aaaggcgtgg gccacggcca gcgcgttttc gccgtcgccg
     5941 tccggcgcgc cccgcaccca tttttggggg aggctcaaag ggccggcagc agcgccggct
     6001 tcccgcgcgc gggcgggctc ttcccctgcg gcgggcaatg cctgctcctg tgggcgaaac
     6061 ccgcaccggc gcagcagcgc ctccatcgcg gcattgcccg gcggcgtttg agtataaacc
     6121 ggccccggcc cggcggcgcg cagcgcccgg cgcagcagga aggttccata gccccgtccg
     6181 cgccattccg gcgcgaccgc gcagccgaaa atttcccctg cccgaagtgt cagcgcaccg
     6241 atgacggccc cattcttttc caatacaaaa acacccgcct ccggcgcggc gcccagcgca
     6301 tccagcgcgg gagcgtcccc gtggcgggca gcgcgcacct ccagcattcg gcacgcctcc
     6361 ttttctttcg ggcttgatac acatttctgt gttcgcaaaa agcagcggcg cctatccctg
     6421 aagcagacgg gaaaaggcgt ggcgtgtttt tacaaagccc cgcttttcca aaaattcctg
     6481 gcttttcagg ttgacgcggg cgcaggacgc ctgcacggaa acacagcccg ctgccttgaa
     6541 ctgcgtggta agcgagacca gcatggcgct gcccacattg cggccgcgcg catcctcccg
     6601 cacataaaac tcatggatga cgcccacagg cgcgcattcc gaaaaaggga aacgcacttc
     6661 ggtgtcggca aagcccacca cctccggtcc ctcaaaggcc agcacgatgc gccgcccagt
     6721 ctgctccagc gcttgggcca gcgcctgcgc gaacgcctct ctgggcagct cgcggcccgc
     6781 cgctgtacac atcagagtat aaaatttttc cgcgtcgggc gcggaaaggc tgcgcaggat
     6841 cataaacgca gacccccttt ttctgttcgt tcccgcggcg ctgccgcgcc gccgtgcccg
     6901 ctgtgccggc gcgcgggccg ctcatggaaa aagccccggc ctcccgccgc agggacgcgc
     6961 ccggagaagc cgcacaggcc cccggcagca ttccccggca ggcagccgga gccgcggtca
     7021 aaatgccagc atatggccgg taaagccgta aatggccaga gccaggatgc cgatatacag
     7081 cagcgtgatg ggcagcccgc ggaacgtggc cggcaccgcg tcgctctgca gcttgcgctg
     7141 cccctccgtc accacctgca ccgccagcac atatcccacg ccgctgccca gtgcaaagcc
     7201 catggtctgc accaggctga agctctggat ggtgctgatg aacagcgtgc ccagaataca
     7261 ggtattgaat gtggccatgg gcagcaccgc cacaatctcc cgcgcgccgt gcaggcgaaa
     7321 aaacaccacc acgaccagca gtacgataaa aaacgcaacc gtgctgcaca gcaccatcac
     7381 cagcgggcgg atatacatgc ggtatggata ctgagccagc cagaggttga caaagtatcc
     7441 aagcggtgcg ctgatgagct ggacagcaca cagcagcgcg ccgaacgtca gggagtccac
     7501 agtggtgtcg tccaccagct tgacaaggcg ggatacgccc aacgcgcggg taaacaccgc
     7561 gttctgggca aacaccgcca tcaccgcgta ggcaaaaaat acgctcaggg ccttcagcac
     7621 ttcgatcata gatgctccgc ccccagactc atttgttgtt ttacgctggc agctgcggcg
     7681 cgccacacgc acatgaccag ggccagcaag ataaaaccgc cgctgggcag ctgcgccatg
     7741 gggaagagcg cgtttttcat tacctgcgtc cccatcagcg cgcccgcgcc caaaaattcc
     7801 cgcaggccgg aaatcagcag cagcatcagg atatagccca gcgaggtgat gatgcccttg
     7861 cgcagcgccg tgcgcacacg ctcgcgctgc ggcctttcat accgtttgag tacgatgggg
     7921 tccgccacca gcaaaggcag gtacaggccc agcagcgcca ggtccgacac cgggtacagc
     7981 aggcccatca cataccgcac gcctacgaac acggccgcgg ctgtgagcgc ataggtgagc
     8041 ccgcggaacc ggaaatacgc aaagcggctg agcagcgccg ccagaacgcg cgtaggtgtg
     8101 agcagcagca gtaccgccac cgaaagcatc acaccgtttt tcagcgtggt agccgccacg
     8161 atgagcggcg cgagcccaag cccctgcata atgaccgggt tgttgagaaa cacacggtcg
     8221 cgccgggcgc gcttttccac atccgcgcgg tggggacgcg gggtctcgcc gataaattcg
     8281 ctcatgagcg cacctccctg cggaagatct tctgggagaa caggcggtcc aggtagccgg
     8341 ccagtgcatt caccgccagc accgcaaagc acacgcccgt gtcatacgag ccgaaataac
     8401 ggaacatcat tgtcataaag cccaggatca taccgtacac aaaatgcgcg cgggtatttt
     8461 tcgggcgcgt caccggctcg ttcaccagaa acacagccgc aaaaataagc gcgccggaga
     8521 gcatctcgta tttgagcgag agcaggcgga tgtccagata ctgccagggc agctccagcc
     8581 ccagcgtgtt cacacgcggg taaaaaaacg ccaccagcgc gcaggagagc agaaagccga
     8641 aagggatgcc cagcgtgatg tcccggcgca tccacaggta catggcgcac gccacgagga
     8701 tgagcaccga agtggcgccg atgggccccg cataattgcc gaggatgagg tcgaacgtat
     8761 ccacgttggg cacgccgccg gcgcgcagcg tgtgggaaac actctccatc acggtggcgc
     8821 tgccggtgtc cagcacgctc atcgtggtga acggcacggg atacaaaaac acctcgcccg
     8881 gccagctgac ggcagcgacg gcatagccga aagccgcggg gttgaacggg taatggccgt
     8941 acccgccgaa agcgtgcttg cccagcagga tggccaccgc cacactgacg acgaccacat
     9001 agtaatgcac ggtggcgggc agcagcagga ccagcaccag cgcagagggg atgctgctgt
     9061 tttccgtttt gtcgcaggga cggtggcgca gccaggccac cagatggtcg cacagcatgg
     9121 ccgtgaaagc cgccgtcagg cacagcaccg cagggcgcgc gccgtaaaaa taggtcgaca
     9181 gtgccagcag cggcagcgtc ataaacacga tgtcccggtt cgcctcatag gcgctggggg
     9241 gctgcagggg cagcgcagtc tcagggctca taaaagtcgc ctcttttcgt tttgggttaa
     9301 acggggccgc cggcggccgc acgcaggctc cttccgggcg tccggctttc cgggccggcc
     9361 cgcacaaagc caggcttccg cctatacgca cgcggcgcgg atggccgaca ttgcggcggc
     9421 gggatccttt gcgccgaaca cggcactgcc cgccacaagc acgttggccc cggcaaccgc
     9481 acacagcggc gcggtggctg cgtcaatgcc gccgtccacc tcgatgtccg tcgaaagtcc
     9541 acggcgctgc gcctcggcgc ggatggccgc gatcttttcc accgccgcgg gcatgaactt
     9601 ctgcccgccg aagcccggct ccacgctcat caccaacacc atctccagcg cgtccagata
     9661 aggaaacacc gcctcgggcg gcgtggccgg cttgatgcac agtgcggccc tgcagccctc
     9721 gtcgtggatg gcctggatgg tctgttccac agggctttcg gcctctatgt ggaacgtcag
     9781 caggttcgcc ccggcggcgc agaacggctt tatatattgc agcgggcgcc ggatcatcag
     9841 atgcacatcg taaaaggccg gcacgcgctt tgccaaagca gccagaaccg gcacgcccag
     9901 gctgatgttc ggcacgaacg cgccgtccat cacgtctaca tgcagcatat ccgcacccgc
     9961 ggccatcacg gcctcacagc tgcggcccag ctccgcaaag tccgcggcca ggatcgaagg
    10021 agaaagtttg atcatacggc acctcgtcgg cctgtcttcc gcccggccct cggacagaga
    10081 caaagcaata agtattgata ttttacctca tttcagccga aaaagcaaat gaggcggcgc
    10141 aaacgggcga tactgcttta taaacgcctg ccggcacgga cgccacaggc gcccgcgccc
    10201 cgctccttcg ccttgacaag cccccgtttg tgcatataat aaagtagtat atatctgccc
    10261 gggtcaccgg gtccaaagga gccttttgat gcctgtttcc gaaaccatcc tgcccgtgct
    10321 catcggcgcg gacatgaact gctacacgct ggcgcgcgcc ttccatgagg cctacggcgt
    10381 gcgttcttac gcctttggcc gctgggccat gggcgatacc atgtattccc gcctggtccg
    10441 cttcaccgcc gtgccggaca tcgacagcgc ggacacactg ctgcgcacgg taacggattt
    10501 cgccgccgca cacgcggaca aaacgctcat cgtcatgggt tgtaccgacg attacgccag
    10561 cctgctcatg gacgtccggg acaagctgcc cgcgaactgc atcgcgcctt acatcacgcc
    10621 ggagctgcgg gacaagctgg tttccaaggc ggatttttac gctctgtgcg acaagtacgg
    10681 catcccctac cccaaaacct tctgcgccga aggccccatg gacgccgccg ccctgtcgcc
    10741 cgaagcgctg ggcttcgcgt accctgtaat cgtaaagccc tccagcagca ttttgtactg
    10801 gaagcacccc ttcgacggca tgaagaaagt gtataccgcc gccacgccgg aggaggcctc
    10861 ggccattctc gcgcaaatct acggcgcggg ctacccggac attgtcattt tgcaggacag
    10921 aattcccggc gacgacagct tcatgcatgt gctgacagcc tactgcgata aaaacaacag
    10981 cgtgaagatg atgtgcctgg gccatgtggg cctggaggag cacacgccca aggcgctggg
    11041 caatcacgcg gccatcatca ccgaatacaa tgagccgctg atgacaaaac tgaaagcgtt
    11101 cctggaggac gtcggttaca cgggttttgc caattttgac attaaatacg acagccggga
    11161 cggcagctac cgcgtgtttg agatcaatct ccggcagggc cgcagcaatt attacgtgac
    11221 gggtgcgggg ctcaacatcg cccgctatgt ggtggaagac cgcgtgcttg gcagcgacct
    11281 cggcccctgt gtgatgaatg aaaaagagac cttctggcac agcgtgcccc gtgccgtggt
    11341 gtacaaatac gtcaaagacc ccgcctttgt ggaaaaggcc cgccgccttg tgcgcgaggg
    11401 ccgggagacc accagctttg gctgcgggta cgacctgcgc tggaatccca gacgcttcgc
    11461 ctattatctg gtgcacatgc agcgctattt caagaaattc aagacctatt acaaatagag
    11521 ctgcgctccg ggcgcaccgc atgggaaagg aggctcctgt atggaacagg cattgcccaa
    11581 ggtgttgggc atcctcggcg ggcttggccc tatggccact gtttattttt acgagatgct
    11641 cacgcgccat acccaggccg cgcgcgacca agaccacatc gacgtcatca tcaacagccg
    11701 cgccaccacg ccggaccgca ccagctatat tctgggccaa agcgcggaga accccttcga
    11761 catcatggcc gcggacgcgg cgcggcttgt cacgttcggc gcggatgtca tcgccatccc
    11821 ctgcaacacc gcccactatt tttacgacag gcttaacgag accatcccca tccccatcct
    11881 gaacatggtg gaggagactg tgctggccgc gaaggcgggc ggcagcgccc gtgtgggcat
    11941 cctggccacc acaggcacgg tgcgcacccg cacctaccag cgcatgtgcg aaaagcacgg
    12001 cctgccctgc gccgtgccgg acgatgccgc gcaggctgcc gtgatggata ttatttacgg
    12061 agacatcaag cagggccggc ccgcaaatat ggacgcgttt gagcgggccg cggcctcact
    12121 gcgcgcgcaa gggtgcgacc gcgccatcct gggctgcacc gagctttcgc tcatcaaacg
    12181 cgacgagggg ctgggcgctt tttatctgga ctccatggag gtgcttgcca gaaacgcgat
    12241 cctcgcgttc ggcaaaacgc ccatcggctt cgactgaagc gcggccaacg ccgccattat
    12301 ttgcaccgcg gccctgctcc gcggcaaaca ggaggtaaca cctgaatgtg tggatttatc
    12361 ggcttttccg gcaatcttga taacaaagag gcaatcttgc agaaaatgat ggaccgtatc
    12421 gtccaccgcg gcccggacat gggcggcatg tacacagacg acggcatcgc gctggggttc
    12481 cggcgcctgt ccatcctgga cctgtccgac gcaggcgcac agcccatggc caacgaggac
    12541 aaaagcgtgg gcgttgtatt caac
//
LOCUS       DAWBSB010000036        12397 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1209_length_12397_cov_3.157997, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000036 DAWBSB010000000
VERSION     DAWBSB010000036.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 12397)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12397)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12397
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1209_length_12397_cov_3.157997"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1882..1891
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4959..4968
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9060..9069
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11082..11091
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2490 a         3883 c         3524 g         2460 t
ORIGIN      
        1 gcccggcgca aggaagaagg gcacgacagc cagtaccagc acagcggcca gcagcgccaa
       61 aacggttttt gtattcctgc tcactgcaat ccctccttca aaaagccgat ctcccgcagc
      121 tcgggcctgg cacagctttc cagccccatc accaccagaa cggtcaagat gccttcgatc
      181 gcggccagcg gcagctgcgt gggcgcgaaa acgcccaaaa agcgcaccgc gctggctgcc
      241 gcgccgcctt ccgcagcggg ataggccatc gcaagctgca ggcttgtgac gcagtatgta
      301 aacagatcgc ccagagacgc cgccagaaat atcgccagct tacggttcac acgcgccttt
      361 ttgcacaggg cataaacacc ccaggagacc agaggcccgg cgatggccat gctgaaggta
      421 ttggccccca gcgtggtgag gccgccgtgg gcaagcagca ccgcctggaa caccagcacg
      481 atgagcccca gcaccgcagc gtaagccggg ccaaacagaa tggcggccag gccggtgccc
      541 gtcatgtggc tgcagctgcc tgtgacggac gggagcttga gcgacgagat gacaaaaata
      601 aacgcgccgg acatcgccag cagcagcagg gccttgcgct gcgcttgcac cgtgcggcgc
      661 agccggacca cacctgccgc caggaacgga aggcacagcg ccccccatgc cgcacagtat
      721 gccggcggaa gatacccttc catgatgtgc atggcatagg cgctttgcgg tacaaacaaa
      781 acgccgccca tgaccacaga aaacgccaaa aatcgttttt cacgcctggt cattgaacat
      841 tcctcctgtt tcttttgttg tgcgccgcaa aaaaaccggg cctttctcat ttatgagaaa
      901 ggcccggtca aacaaagtgt cctaaaaaac ggcgcaccct gatttttacc gcgcaggcgt
      961 tttgtccgtc cgccgcgcgt ccctgccctt ccggcagcat cgccgcaggg caggtcttcc
     1021 ggcttgggcg tcagcgcccc ggcttcgtct tcccgtgtgt acccacagtg acgttttgaa
     1081 gccggaactg ttcccttacg gcggcggtcc cgcacaggtc ttgcacctgt ttccctattc
     1141 tcccggccgc ctcaaagcgg ccgggcaccc tgcagcattt tgcctattca cagtaacaca
     1201 atttttgctc cggcgcaagc gcggagaaac aacccgtcaa aaaacttcca aagccatggc
     1261 ttttttacca agcgcacagg gcgttatgcg cctttgcgcc gctgcccgtc ctgtgcgccc
     1321 cacagggcgc cggtgcggct tttgcggtac tggctcggcg tctgccggaa ccgttcctgg
     1381 aacacacggt tgaacgtccg caggctgtcg aagccggatt ccatgctcac ctccagcatg
     1441 ctcagctccg ttgcagccag caggtcgcgg gcgcagtcca gccgcagggc gttcacatat
     1501 tcccggaagc tggtgtgcag ccgcgcggag aacacgtgcg acaggtgata tttgctgacg
     1561 cccagctcct ttgcaatgtg ctccagtgag agcggctgac ggaagttccg catgatatag
     1621 cgcaccacgc tgctcgtcag gtccatggct tttggctccg gcgccggctc cagtgagagt
     1681 tcgggcagaa cgtgcgccag gatcaaatgg agcagcgcgc gggaaatgct gctgtccgcc
     1741 ccggtacacc ggcagcggta aaggctgtgc agggcgtaca gcacatccgg gtccagcgct
     1801 ttcccgcaca ggtacggccg gcgggggcgg cactgcgtca ggctggggaa ataatcgccc
     1861 atcagagacg tgccgcagac gnnnnnnnnn ntcatatacc ggctgtccgc ttcccggcac
     1921 tggtagctgt gcaccgtgtt gggaaacacc accgccagtt ctcccgcctt cagctcacgg
     1981 aattccccgt ctatcccaac gcagacgcag ccgctctgca ccagaaaaag ctccagttca
     2041 ccatgcagat gggaggggaa attgagccgt ttgcccatct ccacctgaag ctcacgtccg
     2101 cttcgttcct cgtaataagc ataggctttt cgtttttcca tatatgcctc ccacgggatg
     2161 ccccaaaacg caatatttgg ccaaaaaacg attccttctg tccggtatta tatcataccg
     2221 gatgatataa tacagccaaa taaagcacaa aaaccggaat gatttttcgt cgtccggcgc
     2281 ttttgaattt tacaggttag agggtcttta caatgcaaca gctgcaatgg ctttccggct
     2341 gcatgaagga caaacggaaa tggttctggc tggcgatggc gctggccgtt gtctcggcgg
     2401 ggctgtgcgt ggtgttcccg ttcattacgc agcagatcac agaccgggtg ctggtgggac
     2461 aaacgctgcc ggacggttcc gtgcagcgcc agacacagct gctcgtcccg ctggtgtgcg
     2521 ttatgatcgg cgcgcagctt ttgcgctcgt gcgtacgcta cggcatgacg gcgtctctgg
     2581 agtatgtgtc ccagaacgtc cagcaggaca tccgccgcca tttgtacgac aacctgtgca
     2641 cccaggacgc gggcttttat acgcggtacc gcacgggcga cctgatgacg cgcctgacgg
     2701 gcgatctgga catggtgcgc cacacggtgt gctggatctc gtacaacgcg gtggagagca
     2761 cctgcctgtt tctgttttcc atgacatatc tgttcagcgt gaacgccgcg ctgacgctcc
     2821 ttctgctggc cgttgccccg gtgatccttg catgctcgtt cctgttttcg cgcacggtgt
     2881 atcctctgta tgcgtccctg cgggaaaagc tgtcccgcat gaactcggtg gcgcaggaaa
     2941 acatcgcggg caacaaaacg gtgcgcgcgt tcgtgcgcga ggcctatgaa aacgaaaaat
     3001 tcgagaactg caacgaagaa taccgccagg caaacctcaa ggccaacttt cactggctga
     3061 aattcttccc ctacatcgag ggctgcgccc agcttatggg gcttttgtct gtgctcttcg
     3121 gcggcctgtt catcatccag ggaaaaatga cggccggcga cctcgccgcc ttttcgctga
     3181 tgtcctgggg cctgtcggag ccgatgcgcg cgctgggcac ctacctgaac gattttcagc
     3241 ggttcctcac ctccgccgca aaggtcattg aaatttattt tgcccgcacg cggatcgaga
     3301 accccgagca cgggcgcacc gagggcgaat gccgcggcag cgtggagttc cgcggcgtta
     3361 ccgtcgctta cccccacaac agcgccccca cgctgaagga cgtcagcttt tccgtaacgc
     3421 aggggcagac gctggccata ctgggcgcca caggcggcgg caaaaccacg ctggtggacg
     3481 ccgtcacgcg catgctggac gtcagcggcg gcacggtgct ggtggacggc gtggacgtgc
     3541 gcctgtggga ccttcaggcg ctgcggcggc gcatcggcat ggcgacgcag cgcgtgcagc
     3601 tctattccga cactgtcagc agcaacatcg cgtacagcgc gcccgacgac atctccgaag
     3661 acgacgtcgc cctgtacgcc cgcctcgcgg ccgctgattt catctgctcg ctgcccgagg
     3721 gcttcgacac catcatcggc gagcagggca ccgggctttc cggcggccag aagcagcgca
     3781 tcgccctggc ccgcgcgctg gcaaagcagc ctgagatcct gattctggac gacaccacct
     3841 cggcggtcga cctggagacc gaaaagcagc tgcgcaaaaa tcttcaggag cttccctatc
     3901 cctgtacaaa gatcattgtt gcgcagcgca tttccgctgt gcgcagcgcg gaccagatac
     3961 tcgtattgca ggacgggcgc atcgcgcagc gcggcaccca tgaacagctc gcggcctgtg
     4021 agggctatta ccgcgacatc tgcctgctgc agggcgtgca gggaatgccc gacgcgcccg
     4081 cggcaaacgc cccggcaaag gaggtgcgct gaccatggca aaacggaaca ccttcggcgt
     4141 ggacgaaacg ctggaaagca ccttcagctg ggaaatgatc cggcgctgct ttgtgtacat
     4201 ccgccgggag ggacgcctgt tcctgcgcgc gttcgtactg caggcgctgg ccatggtggc
     4261 gggcctgtgc gggccgctgt tctcggcccg cgtgctggac gaggccgtgc ccgcggggaa
     4321 catccccatg gtgctggcct gctgcgcgct catgctggcg tgtatcgccg tcaacattct
     4381 gttcaccaca ttgtccagcc ggtgcgtcaa cgtcatcggc cagaacatcg tccatgacct
     4441 gcggcgcgac ctgtacgagc acctgcagaa gctttccttc tcctatttcg acagccgtcc
     4501 ccacggaaaa attctcgtgc gtgtcatcaa ctacgtcaac tcggtggcca acatcctgtc
     4561 caacgggctc atcagcctgt tcctgcagat gttcaacctg gtgttcattg ttattttcat
     4621 gctgcgcatg gacgcgcgcc tggcgtgcgt cgtgctggcg gggctgccgt tcgccgtcgg
     4681 gttcatcctg ttcatcaagc ccatgcagcg cagagggtgg caggcgtact ccaacaaaag
     4741 cagcaatatg aacgcctacc tcaacgagag catcacctgc atgaagatca cccagctttt
     4801 cgcgcgggag cagtacaacg ccgacgtgta cgacggcctg ctgcgcgatt ccaaacgcgc
     4861 ctggtacagc gccgtcgtgc cgtccatggc cgtttcccct gttatcgatc tcatcagccg
     4921 cagcgtgacg gtggccatca tcgtatacgg catctttgnn nnnnnnnncc gccgttaccg
     4981 tgggcgtgct gctggccatg atgaactacg cctcccagtt ctgggggccc atcaaccagc
     5041 ttgccaacat ctacaacaac ttcatcaaca acgtggcata tctggagcgt atctttgaga
     5101 tgatcgacga accggtcgag gtgcaggacg cccccggcgc cgcagtgctg ccggagatcc
     5161 gcggcgatgt ggagttccac gacgtctctt tcgcctatga aaaggatatc cttgtgctca
     5221 agcatgtgaa ttttcatgtg aagcagggcg agagcgtggc tctggtgggc cacacgggca
     5281 gcggaaaaac aaccatcatc aacctgctca gccgttttta caactgcacc gaaggccggg
     5341 tgaccattga cggcgtggat atctccggcg tgacgctggc ttcgctgcgt gcccagatgg
     5401 ggctgatgat gcaggagagc ttcgtattta caggcaccgt gatggacaat ctgcgctacg
     5461 gcaatcttgc cgccggcgac gaagagctca tccgcgcggc cgagcttgtg tgcgcggacg
     5521 gctttatccg ggcgctgcct cacggctacg acaccgtgct caccgagggc ggggccatgc
     5581 tctcccaggg ggaaaagcag cttcttgctc tggcgcgcac catggcgtcc gacccgaaga
     5641 tcctcatcct ggacgaagcc accagcagcg tggataccaa aacggaacgc gagctgcagg
     5701 agggtgtgaa ccgcatgctc aagggacgca cctccttcat tgtcgcacac cggctctcca
     5761 ccatccgtgc ctgcaacaaa atcatgtaca tcgaccacgg cgagatcgcg gaatgcggaa
     5821 cccatgagga gctgctggcg cgcaagggcc tgtactggca gctctgccag gagatgtgac
     5881 cggccgcccc gcgggcgccg tatacagcaa ccgccgcccc gtttcggggc ggcggttttt
     5941 tgcttctgcc gcgtttattt ttccgtttcc gaaatcagcc ccatcgccag cgcgatgtcc
     6001 aggttcacgc ccagcacgtt cacggtctcc tccccgaaca cgcccagcag tttttcctcg
     6061 gtgttgtccg caacgatctg cgcaagccgt tccgggctga gccctgaagc ctctgcaatg
     6121 gcgggaagct gcacctgcgc ggcggcgggc gagatgtgcg ggtcaaggcc cgagcccgag
     6181 gccgtcatca ggtcggccgg gatatcctcc cgcgccacgc cgggattttc cgccaaaaac
     6241 gcctcgatgt cggcttccac acgctgtgtc agcgcagggt tgctgggcgc gtaattgttg
     6301 gaaccggagc ccagccccgc aaattcgctg ccgtcgttgt agaaccggct gccctgttcg
     6361 tctgtgtaat aggtgttgta gtgataggcg gagggccgcc ccttcatgaa ataggcctcg
     6421 gtgaactcct gtcccaccag cttcgcaccc acagccttgc cgtcggcggt caccaggctg
     6481 ccgcccgcct gatgcgggaa aagcaggccg gaaaggccgc tcagaagcac cggaaataca
     6541 aatccgcaca aaagcagcag aaccagcgtg accacgagag cctgacggat attgcggagc
     6601 attgcttttt tattcgcgtc catgcatatt cctccttaaa agccccaaag cgccaaaagc
     6661 ggatggatga gcatatctat gagcttgatg cctgcgaagg gagcgatcac accgcccagg
     6721 ccgtaaagca tcatattccg ggcaagcatc ctgccggatg acatggggcg gtactttacg
     6781 cccctgagcg ccaacggtat caggcatgga atgatgatgg cattgaaaat gagcgcggaa
     6841 agaatggcgc tgtgcggcgt tgccaggtcc atgatgttga gcacacccag ctgcgggatc
     6901 gccatcataa acatagcggg gatgattgcg aagtattttg cgatgtcgtt ggcgatggaa
     6961 aaagtcgtaa ggctgccgcg ggtaataagc agctgcttgc cgatctccac cacctccaga
     7021 atcttggtgg ggtccgagtc caggtccacc atgttggccg cttccttggc ggcggcggta
     7081 ccgctgttca tcgcaaggcc tacgtttgcc tgcgccagag cgggcgcgtc gttggtgccg
     7141 tcgcccgtca tggcaacgat cttgccctcg gcctgttctt ttttgatcaa tgcgatcttg
     7201 tcttcgggct tgcactcggc aatgaagccg tccacgcctg cctccttggc gattgtggcg
     7261 gcggtaagcg ggttgtcgcc cgtgcacata atggttttga tgccgatggc acgcaggcgc
     7321 ttaaaacgct cggccatgcc cgcctttacc gtgtccttta aataaatgac gcccaaaatc
     7381 cggctgccct cgcagacagc cagcggtgtg ccgcccagag cggacacctc ggcaacgcgc
     7441 cgggcaaggt cctccggcac cttgccgccc atttcggaaa cgtatttctc aatggcgtcg
     7501 gacgccccct tgcgtacgct ccggccgccg ggcaggtcca caccgctcat gcgtgtctga
     7561 gcggtaaacg caatgaagcg cgcgccctgc ggctccccgc agtcctcccc ctgaaggcgg
     7621 gccagagcca gtgtggattt cccctccggc gtgccgtcgt gcaggctcgt cagcgcggca
     7681 cagcgcacca gctcctcccg gcacgcgccg cccacgggga aaaagtccgc cgcaaggcgg
     7741 ttgccgtagg taatcgttcc ggtcttatcc aaaatcatcg tgtccacgtc gccgcatgcc
     7801 tccacggctt tgccggacat ggcgatgacg ttaaagcgcg tcacgcggtc catcccggcg
     7861 attccgatgg cggacagcag gccgccgatg gtggtcggga tcaggcagac catcagggcg
     7921 atcagtgtgg aagtctggag ctgcacgccg gaatacaggg cgatgggata cagtgtgacg
     7981 atgacgatca gaaaaataac ggtgagcgac accagcagtg tgttcagcgc gatctcattg
     8041 ggcgtttttt tgcgggaagc gccctccacc agagcgatca tgcggtccag aaagctgtcg
     8101 cctgggtcgg acgtgatgcg caccttcagc cagtcggaca ccaccgtggt gccgccggtt
     8161 acagagctga aatcgccgcc tgcctcgcgc acaaccggtg cggattcgcc ggtgatggcg
     8221 gattcatcca ccgaggcgat gccctcgatg acctcgccgt cgcccgggat gaccccgccc
     8281 gcggccacca tcaccacatc gcccttcttc agttcgctgg acggcaccat cctttcgcct
     8341 ccgtctgcat caagcaggcg cgcctgtgtg tctttttttg ttttcttcag gctggcggcc
     8401 tgcgcttttc cgcgcccctc cgccactgat tcggcaaagt ttgcaaacag caccgtaacg
     8461 aacagaatgg ccgacacaat actgttgtaa acgcgcgcgt tcggcgtcgt gtctcccagc
     8521 aggccgggcg cgaaggagag caccagcgag atcacaaacc cgatctccac aacgaacatc
     8581 accggatttt tcatcatata gcgcgggtcc agctttgtaa acgctccgac cacggcctgc
     8641 cgcatgatct ccggtgtgat catcttttgc ttgcggttct tactcatagc tctgaaaatc
     8701 ctccttaggg gttgtttgaa aatagcagga acgctgtatt tcccgcaagg atcgagcagt
     8761 tacaaggcca aaagcgcagg aatacctgct gtattccgag gattttggac gcagtaaacg
     8821 tcagttcctg ttgggaaagg cagctttttt gatttttcaa accgctctta gccccacaga
     8881 gccagatgct ctgcaacggg gcccaatgcc agcgctggaa aaaatgtcag cgcggcaaaa
     8941 atgtatacga cgaaaaccag gataacggaa aacgaaaccg tgtccgtccg cagcgtgccg
     9001 gccgattcat tgacaaagtt ctttttcatc agcgacccgg caatggccaa ctggatgacn
     9061 nnnnnnnnng ccgaagaaca tggccagacc ggcggtgata ttccagaaac aggtattatc
     9121 cgccaggccc tcaaaaccgg aaccgttgtt ggcggcggag gaggcatatt catacagcac
     9181 ctggctcagg ccgtgaaagc ccggattggt gatgccctcc cgcccggctg cggtgcccac
     9241 tgccagcgcg gaaaacgcaa ggatgagcag cgggtggatg atgatgcaca gcgcggtgag
     9301 cttcatttcc cggccttcga tctttttgcc caggtattcc ggcgtgcgcc cgatcatcag
     9361 gccgcagatg aacactgcca gaatggcgta caggatcata ttcatcagac cgacgccctt
     9421 gccgccgaat actacgttca gcatcatgtg cagcagaggg atcatgccgc ccagcggcgt
     9481 cagggtgtcg tgcatattgt tcaccgtacc ggtcgtaaaa gaggtcgtga cggtggtgaa
     9541 catcgccgac tgcgcaatac cgaagcggac ctccttgccc tccatactgc ccatggcctg
     9601 gtccaggccg atatccgcca acgccgggtt gccctggctc tcggcccaga agcatacgct
     9661 caggccgatg acgaacagga tgcccatggc ggcaaaaatg ctgcgtccct cgcggccgta
     9721 gatggccgcg gaaaggccgc cgcgcgcacg caggccgcct gccctttgca tgccgtccgc
     9781 cgcttcctgc ctgcgggcgt gcaccatttt accgaaggtg atgacgcatg cgccgggcag
     9841 gagcatcatg gaatacagct cgatcaggtt tgagagcatc gtggggtttt ccagcggcgt
     9901 ggacgagttt gcgcccagga agccgccgcc gttcgtgccc agatgcttga tgatctccag
     9961 cgcggcgatg gggcccatgg caatgacctg aaatgtgccc tccagcgtct ccaccacaac
    10021 attgccttca aaattctgcg gcacgccctg ccataccagc agcaggccgc ccacaatgga
    10081 aaacggcagc aggatacggg tggtgatgcg caccaggtcc gcaaaaaaat tgcccacgtc
    10141 gttttttgtc ctgcctgcaa ggccgcggat gaacgcaacg caggcagcat atccgcttgc
    10201 ggcggacacg aacatcataa aaatgatgac cagcatctga gacaggtagg aaagcccgga
    10261 ctcaccggaa tagtgctgga gattcgtgtt cgtcatgaag ctgataatgg tattgaacgc
    10321 cagcgtgggc tccatattct caatattgtt cgggttgaaa agccctgcgc tctgcacgcg
    10381 caggatcaga tacccgatga acaccatcac ggcgttcgtc cccagcagag ccagggcata
    10441 ctgtttccag ttcatcccct tcccggggtc cactccgctg attttataga tcgctccgtc
    10501 cacacggtca aagacggggt ctgcaaaagt gtgtttcccc gccgcgatgt gatacagata
    10561 cacacccgcg ggaatgacaa tgagcaaata aatgctcact gtcagtgcaa gctgtagcat
    10621 gacaagctcc tcctgtttat ctcagtttcg ctcacagcga atcaaaattt ttccggatgt
    10681 accaatgcat agatcagata acagcccagc agcagcacga tcccacccag taccagcatt
    10741 tatgtatcct ccttgcggcc tttttccccg cgctcactcc gcggcgtcca cctgcttctg
    10801 gcaccatttt gccaaaagcc atacaagccc gaagcaggcc agcaatgttc cggccatcag
    10861 aacatccatc ataatccatt cccttccttc ccttatcgcg gtatgcctca ttatagggca
    10921 taagctgtaa gtgtgcgctt aacgttcgcc gccggatgtt aaggttgcgt tagcgcccgc
    10981 aaaggcgcaa aaaagaagcc ttccggaaaa ccggaaggct ttttcccacg ggcgcggccc
    11041 gctgtttttt catttttcat aaagggagcg gagcataaaa tnnnnnnnnn ngcataaaat
    11101 accggaagct cccgctttac ggctgccagg gagcgcgctc catccacttg ccgttggtaa
    11161 agtccggaat gggcacggga gcgccgccca tggccacgct ctgctcgctc aggcaggaaa
    11221 tactcatcca tgccgccgca tcatatacgt cgatgggcgc aggcgcgccc gcctttgcgg
    11281 cgtcgaagaa cgagcggaac accagccagt ccatgccgtc atggccgccg cgcacgcctt
    11341 cggccagata ttttttccac accggatggt cgtactgctc gcggtactcc tcggcgctgt
    11401 tccagcgctc gttccactta aaatcatact cgttgtcttt gccgtctaaa aagacgctct
    11461 tgttgtcctc catgtacatg ccctttgtcc cctgcacatg gaacccgcgg gaataggccc
    11521 gcggcaaggt ggtgtccagc gtcaggcaga tcgtctcgcc ccgggcgcat ttgatgatgg
    11581 tcgtcaccac gtcgccctgg gcaaaattca tttttgcagc gtcgtagtcc gggccttttt
    11641 cccgcagcag atattcgtgc agccccgcgg ccttggacgc cactgaaaca agcgtcagca
    11701 tgcggttgcc gcggttgatg tccagcacgt ttgcaatggg gcccagctcg tgggtggggt
    11761 agttttcgca gttccggtac aggtagttgc ggaagcggta gtgccgcatc tcgcgcccgg
    11821 tggagacctc ctcccgcagg tcgtggcggt aacctccctg gcagtgcacc acctcgccga
    11881 acaggccctg gcgcaccata ttcagcacca tcagctcgtc gcggccaaaa cagcagtttt
    11941 ccagcatcat gcagggtacg cccgtttcct cgtgggtgcg caccagcttc cagcagtcct
    12001 ccacagaata tgcgcccgcc acctccacgg ccgtatactt gcctgcgcgc atggcgtcgc
    12061 aggcaaggtt cagatgggat tcccacgacg ccataatgac cacagcgtcg atttccggca
    12121 tggccagtac ctcgcggtaa tccgccgcag cgcggggcgc gggctggccc gctgccgtca
    12181 caagctgcac gcccttctcc cggcggtcct cataattgtc gcacacggcc agcacacgaa
    12241 cgcccggctg gggcaggata cagttttcca acaggttgga gccgcggttg cccaggccga
    12301 taacacctac attcaatact tctttcataa atggatactc cctttccgtt tcaaaagcgg
    12361 cccgcacagg ctctgggccg ttatttcctt cgtctgc
//
LOCUS       DAWBSB010000037        12361 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1215_length_12361_cov_3.359662, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000037 DAWBSB010000000
VERSION     DAWBSB010000037.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 12361)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12361)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12361
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1215_length_12361_cov_3.359662"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    9369..9378
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9898..9907
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2376 a         4016 c         3567 g         2382 t
ORIGIN      
        1 gagacatccc gccgcgcggc ggctttgatg tatctgtatc ctgttctttc agtgggcgca
       61 atgctcgtac ccgtgggaga tcagccacgc gttttttctc tcctctataa tttcattccg
      121 gccgttcagg ctctggccgc agtttgcctt taccccgcag tccaaagctg ccagataatc
      181 cagaatcttc tcctccgtga tctcttccga cgggatatgc tctgccgccc accaaaccgg
      241 gtccttctgt tctgtcggct tgaaggcaag cgccacggaa taatatgcaa agggctggcc
      301 cgtttcttcg cagacagcct ttgttttcag caggtagtcc gccaattccg catctgcagg
      361 agccggcgcg ccaaccgcag aaaagcttac gctcagctct atcggaagcg gaagcccggc
      421 acggtcgtat tcctcgtctg tccgaaagac cggcgcgtcc gggtcaaaca aagggccttc
      481 ctgcgttgtg taaaagcctg catgaacttc ggcttctccc gtcagcgctc tcagccgcgc
      541 cgccgttccg ccgctttcca gctccgagtt caaaagattt ttgaaccgtt cgtaggtgtt
      601 gcagcccgaa cggactgctc cgtctatttg gctgaacact gctccatcgc tgaaatatgc
      661 cggaaattca acatcctcgc ttgcttcttt gcgaaccgta cacacataat ccatgcccgc
      721 cgatttgtcc gcccggacga cccactgata acgggcaggc ccgacaacgt agccttcacc
      781 cggatggtaa gccctataat gcgcggtaat tttctgttct gtataccagg cgatcagagg
      841 atttcccgtc tttcgccatg tccaccatac aagtcctccc aaaagaatca gcgccaacag
      901 aagcgccagc gcacgcgttg ttttcgtacg cggaacgcga gtcctgcctt tgtgcgtctc
      961 gcccgctctt tcgtcaatgc tttttgccgt ctgcatattt ttcctccttc ctgtttccaa
     1021 acgcaaaatg cagcagcccc gcgattgcac agcctatggc cagcggcccg gcaaccgcca
     1081 gcgccataga cacatacagc accactgaac cccactgcag cgattgcatg tttgggataa
     1141 aaaaatacaa aagcacaaac gccgccgctg tagccggcac cagccagcac ctgcgccgca
     1201 ggacataata acacgccgcg cccaccagcg gcagccacca tgcaagatac acgtcgcccg
     1261 cgcgggaggt gagccacagc gcgcccagca cgcccaccag cgcgaacagc acgctttgcg
     1321 ccagcagcct ccagcgcagc ctgcgcagta cccgcgcatc gtccggcatg ctgtgctcgg
     1381 gcggcgtttc gctttccagc atcgtgcggc agccaccgca acgctccgta tgcgcgcgta
     1441 ccagcgcttc gctgtccgcg ctggccacgc cgtcccgtac cagcggcatc aggtcccggc
     1501 atacctcgca ggaaatgtca aagcgtatcc ctcgctctgt ttgtgttttc atagctctgc
     1561 cccctccttc tgcaattgct cccgcagcca gtggcgcgtg cgaaaatcca gcgtgcgggc
     1621 cgcgctttcg ctgacgccgc acctctgtgc gatctccgcg aacgaatatc cctgcgcccg
     1681 cagccaaagc acctctccgg cgggcgggcg gcgcgtttgc agcagttcct ttgcccgcgc
     1741 ggccagcgcc cgggcgtcca cctgcgtttc aaacggaggc gcgcccgggc cgccctcctc
     1801 cacataccgg gccaaaagct catcgccgga aagtacatgc cgtttgcgcc gcagcccgtc
     1861 gatccaaaca tgccgtgcaa tgctgaacag ccaggtgcgc acacatgcgc ccttccgaaa
     1921 gccgctcagc cctgtgagcg cccgcaggaa cgtctcgctc agcaggtcct ccgcaagggc
     1981 ggcgtcccgc gtcaggctgc acaggtaacg gtacacatcc tgccggtact gggtatagat
     2041 ctgttcgatc tcctgcatcg ggacgcctcc tttcagcatg ttagacgcac gcgcagacat
     2101 tttgtttcag ctttcggaaa actttttgcc tccttgcaaa acaaaacgcg gcgcagagca
     2161 aaaaatgctc tgcgccgcgt ttcatcccaa caggccgcga gccgttcagc ggatggccgc
     2221 gaagcctttt ttcagatcgt ccagaatatc gtcgatgtgc tccagcccca cggacaggcg
     2281 gataagcccc ggcgtgatgc ccgcggccac aagctgttcg tcggtgagct ggcggtgggt
     2341 gctgctggcc ggatgcagca cgcaggtgca gatgtcggct acatgcacca ccttgttggc
     2401 cagggacagg ctgtccatga acttgacggc cacctcgcgc ccgccgcgaa tggagaacga
     2461 gatcaccccg ctgcacccgt cgggcaggta tttttttgcg gctgcggcat aagggctgcc
     2521 ggacagcgcg ggataattga caaactccac ctccggctgc cttgccagga aggcagcggc
     2581 ctgttccgcg ttgcggcagt aacgctccat gcgcacggcc agcgtctcca gcccaaggtt
     2641 cagcagaaac gcatcctgcg cggaggggta cataccgaaa tcgcgcatca gctgcacccg
     2701 cgccttggtg atatacgcag ccctgccgaa atccctggta taggtcacgc cgtgatagct
     2761 ctcgtccggc gtgcacagct cgccgaattt tccgttttcc cagttgaaat tgccggaatc
     2821 cacgatcacg ccgcccacct ggatggcatg gccgtccatg tacttcgtgg tggagtgcac
     2881 cacgatatcc gcgccccact cgatgggacg gcacagcaca ggcgtggcaa aggggttgtc
     2941 cacaagaaac ggcacgccgt ttctgtgggc ggtgcgcgcg aatttttcaa ggtccagcac
     3001 agcgatggca gggttggcaa tggtctcgcc gaacaacgcc tttgtgttgg ggcggaacgc
     3061 cttttcgatc tcctcctccg gcgcgtccgc gtccacgaag gtacattcga tgccgaactt
     3121 cttcagcgtg accgcaaaca ggttcaccgt accgccgtaa atggtggagg cggcgacgaa
     3181 atggtcgccc gcggtgcaga tgttcagaac cgcaagcaga ctggccgcct gtccgctggt
     3241 ggtgcacagc gcgcccacgc cgccctccag cgccgcgatt ttctgttcca ccgccgcgac
     3301 ggtggggttg gagatgcggg aatacatgtg gccgtccgcc gaaaggtcga acagctggcc
     3361 gatgtgctcg gtggaatcat aggtgtaggt ggtgctctgg tagatcggca gggccttcgg
     3421 ctcgccgttg ccgggcttgt agccctcatg caggcatttt gtttcgatgt gcatggtgtt
     3481 tttcttcccc tctcacgaat actttttcac gatggacgcc atttcgtccc aaacgttttc
     3541 catgtccgcc accagcttga actgggtgcg gcagcgctcc aggttctggc gcgcccggct
     3601 caccttgaag taggtatcgc cctgcagata gtccgtcaga aaacggatgc cgcactccag
     3661 cgtcatcagc ttggcgcccc agggcaggta ctctttttcc gtgtccgtca gcgcggcgcc
     3721 cgcggcctcc agataaccgc gggtatacag ctcaaacagg tacaggtcaa aatgcacctt
     3781 ggaaacgtcc ggctcgtcct cagccgccgt ggacgcgccg aagcggatgg aatcgccgta
     3841 atcgttcagc gcaagccccg gcatgatggt gtcgaggtcg atgacgcaca gcgcctcgcc
     3901 ggtgtgggca tcaaacaaaa tattgttcag cttcgtgtcg ttgtgcgtca cacgcagcgg
     3961 cagctttccc gcgtccagca agtccatcag caccgcgcag tccgccctgc gccgctgcac
     4021 aaaggcaatc tcctcccggc aggtccgcgc gcggttcttc acgtcgcgct ccagcgcctt
     4081 gtcaaagtct gcaagacgct tgcgggtatc atggaatttc tcgatggtct cgtgcagcgt
     4141 cccggccgga taatcctcca gcgcgcgcaa aaagccgccg aagctcttgg ccgaatggta
     4201 gaagtccatc gggtccgtca cgctttcgat gcacacggaa tccggaatat agttatagca
     4261 gcgccacgcg cttcccaccg cgtcctcaaa atagttgtcg cctgttttcg tcttgatata
     4321 attcagcgtc tcgcggtccg cgtcgccgcc gcgcgccagt atctttcggc gcaggtattc
     4381 cgtcacgccg aaaatattct ccatgacggc gcgcgggtcc ttaaacacac ccgtgttgat
     4441 gcgctggatg acatagcgca gctcgtcgcc gccctgcgcc gccatgtaca cggcataggt
     4501 gtcgttgatg tgtcccgcgc cgaagggctt tacatagctg gccgtctcgc ccagcttgaa
     4561 agcgaacagc gcgtcctcca ggttgtggtc ctccaccccg tcaatgtgcc ggcgggtgga
     4621 atacagcacg gtggccgcct cggtgcgcgc gcacggctcc atgtggcacc aggccttgat
     4681 ggtggcgtgc agattgacgt gcacgccgtc ttccaggacg ttatcgtggt ccaccagcgc
     4741 gccgctgttt acaaggcatg ccgcgcccag cacagtattg gtgttgatga tggccccgtg
     4801 cagcacaagg cagcccggcc caagcaccgc ggacgggctg acgatggctg tgggatgcac
     4861 cacgctgggc acacggtaac ccgcgtccag caggcgacgc gtccacgcca ggcgcaggcg
     4921 gttgtcgcca aacgccgcca cggcctcagg gtattcaccc gtgagcgaag tgtagtccat
     4981 gcatttgcct gcaacgtccg cgccgcggac ggcgtcgtcc agaaacgcca cgccgtccca
     5041 gttccctgcg gcgcgcgcca cttcccccac catctggcca aggccgccgg cgcccaagat
     5101 caaaagttta gacatgctgt ccccccaatg gtaaaaatgc cgcgccccgt tcgagcacgg
     5161 tgccggattc tatcttcatc tgcgcgggca cggtgttgcc ggccttgaca atggccccgg
     5221 gcgcaaggtg ggcgcacgcg ccgaccacac agtcgtggtc cgcgatcgcc cccatgttca
     5281 cgatggcccc ctgctccacg acagcacacg ctcccacagc ggccagcgcc agcaccacag
     5341 ccccgggacg gatatccgcc gtggggctga cataggccct cggatgcacc agcgccggca
     5401 ggctgtagcc cgccgcggcc agtttttcca gccaggcggc gcgcagcgcg ttggcgccaa
     5461 aggccggata cgcccagcgg aattcccccg caaagcgtat atagtccgta cacgggccga
     5521 ggacatccgt tcccgtgcat ccgtcatcca ggaacgccac acgcccgaaa acgcccatag
     5581 cccgcgctgt ttcggccgcc gtgcggccgt agccacccgc gccgaggatg atcaggtttt
     5641 ccatccgcag cctcccatac cgcctccgca ccgcgcggcc ggaggcattc acattcttgt
     5701 atatgataga atatcatacc atatttatac taaaaaaaac agaccgcttc ttcaaaacct
     5761 ttcgcagccc cctcccggaa aactccgcag gggcagcaga cgaagcgccg ctgcggagct
     5821 gcggctcccc gtgcattgca ccctcgtggg gcgacgcaaa atgcttcggc gggggcaaaa
     5881 cccacgaaat tcgtatactt acacaacttt gcgcgggaca gagggcaaaa atttggcggc
     5941 gcggcaagga tttttttggc aaaagctctt tgaaaaatgc gcatttctgt tataatagct
     6001 ctaaagtgcc gctttgcggc gggcctgccc ggcagaccct gaaagcgcgc tgcgctttcc
     6061 tttcggcttt tgtaacatgc aaaaccatga cgcgcatggt tttgttgtgg ccgcttcgcg
     6121 gcgacatcat gttataatag ctctaaagtg ccgctttgcg gcggcgggct gctgtattgc
     6181 ggcccgcttt tccggcccgc gcccgggcca atcggtttta atgaggagag gaaaatggga
     6241 gaaacaattc tggaactgca aaacatcgca aagcatttcg gtgataccga cgtgctgaac
     6301 ggtatcagca tctcaatcgg caaaggcgaa ttcatcacgc tgctcggccc ttcgggctgc
     6361 ggcaaaacca cgacgctgcg catcattgcc gggctggaaa cgcccgacga gggccgcgtc
     6421 attctgaacg ggcaggacgt gacggactcc gaacccaaca aacgcgacgt gaacaccgtg
     6481 ttccagaatt acgccctgtt cccgcacatg acagtggccg cgaacatcgg ctacagtctg
     6541 aagctgaaaa aacgtcccaa ggacgagata aaaaaagccg tggacgacgc gctggccctt
     6601 gtgcagctct ccggctacgg caagcgcatg cctgcggagc tttccggcgg gcagcgtcag
     6661 cgtgtcgcca ttgcccgcgc cgtggtgaac cgtcccagcg tgctgctgct ggacgaaccc
     6721 ctcggcgcgc tggacctgca gctgcgccgc caaatgcagc tggagctgaa ggctttgcaa
     6781 aaacaactgg gcatcacgtt catctacatc acccacgatc aggaggaagc ccttaatatg
     6841 tcggaccgca tcgccgtgat gaaagacggc cggttcgaac agctcggtac tccggcggag
     6901 gtgtatgaca gcccgcgcac cagctatgtg gcgcgctttg tgggcaccgc caatgtgctg
     6961 acgggcacgg ttgaggctgt ggaggacgac gtgctgaagg tagcctgcgc aggcggctgc
     7021 gcgggcgcgc tgcgccgcgg atattccttt gcccccggtg cgcccatcac actggccgtg
     7081 cgcagcgagc acgtggaatt cacaaaggac cccgctgcct gcggtatcgc ggccgttatc
     7141 cgggaaaaga cgtttgccgg cggcatgctg cgcatcgcac tggagctttc cgacggcacg
     7201 acgctcatcg ccagccgcca cggcattgat atggatgtgc agacgggcga cgcggtgcgc
     7261 gtgacctggg cgccggccag cgccgtgccc gtggacgtgg aggcggaaga agcgaaatga
     7321 aagcagcctc cacccctttt aaaaagaaaa aaaagcagag cggcgtctgg ctgacgctgc
     7381 tgccgctcta cctgttcacg ctggtgttcg tggccgggcc tctggtgtat atgttcgtac
     7441 tcagcttcca gacgcgcgcc gaggtatggg gtgtggtgaa ccagttcaca ctggacaact
     7501 acaaaaatat ttttcagccc gtgtacctga acacctttgt ggagtccttc aagctggcgc
     7561 tcaccagcac gctgctcatc tgcgccatcg gctatccttt cggctatttt atggcaaagc
     7621 tgcccgcgca gtggaaaaag cgcgtgatgc tgctgctcat gatcccgttc tggaccagct
     7681 cgctcatccg gctgtacgga tggatcatca ttttccgtgc aaacggtacg ctggataagc
     7741 tgctgatggg cctgggcctt accgacggcc cgctcaagct gctgtacacc taccccgccg
     7801 tggtggtggg catggtatac gcgctgcttc agttcatgat cctggccgtt tactccagcg
     7861 cggaaaaaat ggactggagc cttgtggagg ccgcgcggga cctgggcgct acgccgctga
     7921 aggcctttct taccgtcacg ctcaagctga cgctgccggg cctgctctcg ggtgttatcc
     7981 ttacgttcat cccctccatg ggcctgttct tcatcgccga catcctgggc ggcaacaagg
     8041 tggtgctggt gggcagcctc attcaggacc agcttatgaa agcacacaac tggccctttg
     8101 cggccgcgct gagcgtggtg ctgatgatcc tgaccagcct gatgatctcg ctgtacaagc
     8161 gtgtgacaca caccacggaa ctggagggca tcttatgaaa aaccgtacaa aactgcccaa
     8221 catctatatc ggcgttgtca cggccgttct gtatctgccc atcgtgctcg ttatcatcta
     8281 ttcgttcaac gaaagcaaga tcagctcggt gtggggcggc ttttctctca aatggtacga
     8341 aacgctgttc agggacgccg ccatgttcga ggcgctgggc aacagcatcg ttctggcgct
     8401 gtgcgccagc ctcggcgcgg cggtcatcgg cacgctgggt gcttacggct tcaccaaggt
     8461 gcagcttcgc accaaaagtg ccgtggaata tgtgtccatg cttcccatca tgatccccga
     8521 gatcatcctg ggcatggtgt tcatgacgtt cttcgcgctc atcggcctgc ccttcggcat
     8581 gacgacgctc atcctcgccc atactgcctt ttgcgtccct tatgtctata tgctggtgaa
     8641 agcgcgcctg gtgggcatgg acaaaagcct ggcggaagct gcgcgggacc tgggcgcggg
     8701 cgaggtgcgc gtgttcttcg atatcacgct gcccctgctg gtgcctgcca ttctgtccgg
     8761 catgctgctc agcttcgcca tgagcctgga cgacgtcatc atcagtatct tcgtcacggg
     8821 cgtcaacgta aacacgctgc ccatcaaggt ttatacgcaa atcaaaaccg gcgtcacacc
     8881 ggagatcaac gccctgtgca cattgatgct gggcgctacc atcattctgg ttgttctctc
     8941 gaatgtcctg ggtaaagtgg gaaagccccg gaccgggaag cagcataaag agaccgcgga
     9001 gcgttccgcg gctcccgtcc cggcgcagcc gcagtgattc tgcagccgcc gcagcaacac
     9061 cataaggagg aaaaccaatg aaaaaattct ttgcactggc actctctctg gcgctggcgc
     9121 tcaccgcgtt cgcgggctgt tccagcacgt ccgcttccgc ttccacgccg tccgcggctt
     9181 ccggcagcac ggccggctcc gcaggcacgg ccgcttccgg cgagctgaac ctgtacacct
     9241 gggagggcat gttcccccag gaggtgctgg acgcctttac cgccgagacc ggcatcaagg
     9301 tgaactattc cagcttcgat accgacgaga ccatgctggc aaagctggag accgccaagg
     9361 gaggcgacnn nnnnnnnnac tatgacgtcg tcatcgccga cgactacatc attgaaacgg
     9421 ccatcgccga gggcctggtg atggagctgg acaaaagcaa gatcgccaac atcggcaacg
     9481 tgaaccccgt ataccagggc cagttctttg acccggagga cgcctacacc gttccctacg
     9541 gcgcgggcgt gcagaccatc gtctacaacc ccgatctggt ggatattgag atcaacggct
     9601 acgaggacct gtgggacgcc gcgctggaaa gcagcctggg catcaccagc aacttccgcg
     9661 tgatgaacgg catggcgctg aagatcgcgg gtgaaagcta caacaccgag gacgtggccg
     9721 ccatcgaggc cgcgggcgca aagctgcttg agctggcccc caacatccgc ctcatcaagg
     9781 acgacaacct gcaggatgac ctgatctccg gcgagatcag cgccggcgtc atgtacacca
     9841 gccaggtgac catggcctgc ctcgccaatc ccgacctgaa ggtcgtttac ccctccgnnn
     9901 nnnnnnnagg gcatcggctt cggcatcatg gcccagttcg ttcccgtaaa cgcccccaac
     9961 cccgacgccg cccacgcgtt catcgattat atcctgcggc cggaggtctc caagcagtgc
    10021 tttgagtata tcggctatta ctgcaccaac caggctgcgg acgcgctcat cgaggacgcg
    10081 taccagtcct tcctgacgct gcccgaggat ttcagcgctg acaacatgga aatgatccag
    10141 aatatttccg ccgaagccat ggaagcccac accaaagtgt ggacagagtt ccgcacggcc
    10201 tgcggccaat gaagcgcgtc tgaatatccc cggcccgtgc gccggataaa aaaactccgg
    10261 gaaatggggg ccgtcttttg acggcccttg ttcccgtatt ggggaggttc aaaaccatgg
    10321 atgctgttga acagaagatc cttgccgccg ttgacgccca gcgtgaaacg ctgcttgcct
    10381 ttgcacagga tatcggcgcg cacgccgagc cgggcttttt tgaaacacgc accgccgccc
    10441 gtgtggccga actgctgcgc ggctgcgggc tttcgccgca aaccggtctg gcccgcacag
    10501 gcgtaaaggc cgttttgcag ggcggcgcgc ccggcccctg cgcggccgtc atcggcgagc
    10561 tggacggcat tctttgcccc gcgcaccccg ccgcaaatcc cgaaaacggc gttgcccatg
    10621 cctgcgggca caatgcgcag ctcaccgcgc tggtgggcgc cgcgctggcg ctgagcgcgc
    10681 ccggggtggc cgaggcgctt tcgggcagcg tggcgttttt tgccgttccc gccgaggaat
    10741 atgtccccat cggcacgcgg caggcgctgc aaaagcaggg cgtggaattc tgctgcggca
    10801 aatccgaact gctgcgcacg ggcggcttcg acggcgtgga ccttgcgctg accacgcatg
    10861 tacatatgat tccctgcgaa agcgacctcc ttttgggcaa cgtggcctgc aacggcttca
    10921 ccagcaaaac ggtcgtcttt cacggcaagg ccgcccacgc ggccactgcg ccccacgcgg
    10981 gcgtaaacgc cctcaacgcg gccgcgctgg cgctgaacgc cgtggggctt ctgcgggaga
    11041 ccttccgcga atgcgacacg gtgcgcatcc acaccaacat ccgcgagggc ggcgcggcgc
    11101 tgaatgtggt gccggagacc gtggtaatgg aggccatggt gcgggccgcc acactggtgg
    11161 cgctggacga cgcggcccga aaattcgacc gggcctgcac ccacgcggcc gaggctctgg
    11221 gcgcgcacgc cgagattctg acctatcagg gctacatgcc tgtgcgcccg gccccggcgg
    11281 accccgcttt gctggcggcg gccgccgcgc tgcccgggct ttcggcccaa tgcgccgagc
    11341 cgggtatgca gaacattgcc agcaccgacg taggcgacct ctcccaggtg atacccgtgg
    11401 tgaactttac ccacggcggt acggcgggcg cgctccacag cgcggacttt gccgtcacgg
    11461 acgtggacaa ggcctatatc gctcccgcca agatgatggc tctgacggcc tatcatctgc
    11521 tgaaggacgg cgcggtgctg gcgcgccgga caatggacgg attcaccccc gtgttcacac
    11581 gggaaagcta tatcgaaaca gtaaggaggc agggctgatg gcacagcgca tctgcttttt
    11641 gggacgcggc ggcagcggaa aaagcaccgt cgcgcagaac ctgggccacg cgctggccct
    11701 caaagggtac cacgtgctgc tggtgggcaa cgacatcagc ctttcctcca cggtgctgct
    11761 gcgcggagaa gcggatatct cccccgcgct ggaggattac cgggaacact acgagatcga
    11821 cctgaacgac tacgtcatcc ccacgcccag cggcgtatac tgcctggagc tgggcagcat
    11881 cgaccccggc gtgggatgcc tggcccgcgg cgtcacgctc attgatgaga tgctggatac
    11941 tcagggcgtc tccaattccc tgcagctcga cttcatcctc tatgatatct ccggggaaac
    12001 gccctgcacg ggctacatcc ttcccatccg cgagggcgtg atgcagcggt gcatcattgt
    12061 caccaacgga agctttgcct ccgtcaccac ggccaacagc attttgcagg ccatcctgcg
    12121 cgccgcgggc gcggacaact tccccgtgca gctcatcgtg aacaatgcct cctgtgagga
    12181 gacgaaagag gagcttgcca gctatgcccg gcgcgccacc atcgagattc tggccagtct
    12241 ggattactgc aaagagctgg aatacagctc ccttgccggc aaaacggtgt tcgacaccgc
    12301 gcccgatagc gcctgcgccg cacacctgcg cgccgttgcg gacgatcttc tgctctccac
    12361 c
//
LOCUS       DAWBSB010000038        12361 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1216_length_12361_cov_2.303592, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000038 DAWBSB010000000
VERSION     DAWBSB010000038.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 12361)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12361)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12361
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1216_length_12361_cov_2.303592"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3310..3319
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8684..8693
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2457 a         3872 c         3479 g         2533 t
ORIGIN      
        1 tttttcttcc tttttctgcc gtgcttgggg gcttattccc cagcgatctc gatgcggcgc
       61 gcctgcggaa cctgcgccgc ctgcttgggc agcgtcagct ccagcacacc gtttttgtat
      121 gcggcgctga tgtgttccgt atccacgccc gaaacgtcga agctgcggct gaacgagccg
      181 aacttgcgct cccggcgcag atagttgccg tctttgtcct tttctccgct ctcgctgctg
      241 tggcgcgccg tgataacaag cgttgtaccg tccagatcaa tgccgatgtc ctcgcgctca
      301 aagcccggca actctgcctc cagcagatag ctgtcgcctt tgtcctgcac atcgcagcgg
      361 aactgtgcca tattggaaat gctgccatcc cagaagctgc gctccacatt atcgagataa
      421 ttgaacaaat tgcgttcatt gcggccaaac ggaatcaagt caaacataat ctgctccttt
      481 tctctgtgcg tactttattt ttcccggcgc ccgcgcgcag gcaggttgtt ttgccggaac
      541 atgttctttt ttgttcccct tcggaacaat ttttattata aaggctgttc atgaactatt
      601 ttcgctaaat ttatgaacaa aatatgaatt tttagcactc tattatatcg agtgctaaaa
      661 attttcaact tgcaaattac actgctcttg tgtataatga atttatctgc ccgcgatgct
      721 tataatgtat aaaaagcgtc tgcagacggt tttacaacac cccaaggagg gatatcatga
      781 gctttctttc caaactggtc ggcatcggcc tcgtggccgg cgccgccgtg gccgctgtta
      841 agctgatcga gaaggtttgg ccgcaggacg atgcgctgga cgacgtagta tacactgttg
      901 aaacgggcga atttccgcag gaggacggca cggacgccga agatgcgccc gaagcgcctt
      961 accgcccttt cgatcccgac gcagccctcg cgcccgtgcc gaatgtgaac cctgtggagg
     1021 cgccgcccgc cgtgcccgtg actgatccga ccaaaatcgc ggacccggcc gatttccagg
     1081 actgggatga gcttggctgc cagggctgaa cgttccgcgt tccgccgtat ttgtacgtta
     1141 cacgccgctg ccctggatgc agcggcgttt tgcatggagg taacagcaat gacaaaaatc
     1201 acaatttttt ccggattcct gggcgcgcgc aagaccacgc tcatcaaaaa actcatcccc
     1261 acggcctaca agggcgaaaa gctggtgctc attgaaaatg aattcggtga gattggcatc
     1321 gacggcggct ttatgaaaga cgccggtatc gaagtaacgg agatgaactc cggctgcatc
     1381 tgctgcagcc tggtgggcga ttttggcgcg gcgctgcaaa aggtactgga cgaataccat
     1441 cccgaccgta tcctcatcga gccgtccggc gtgggcaagc tgtccgacgt catccgcgcc
     1501 gttcaggccc tgcatgccga tgtggagctt gcgggcttcg tcaccgtggc ggatgcatcc
     1561 aaatgccgca tttatatgaa aaacttcggt gaattttaca acaaccagat cgagcacgcc
     1621 agcgccatca ttctgagccg cacgcagaac ctgccggagg ataaactggc cgcggccgtt
     1681 gcgctgctgc gcgggcacaa cgaaaaggcc cccatcgtca ccacgccgtg ggacgcgctc
     1741 accggcgcgc agcttttgga cgcgatggaa cagaaaaaca gcctgatggc ggagctgacc
     1801 gctcttgccg gggaaactgc ccacacgcat catcaccatg aagatggctg tacctgcgag
     1861 gcgtgccgac atgagcatgg gcatgatgaa caccgccacg accacgggga tgagcattgc
     1921 cacgacgaag cacacgcaca cggtggaaac tgctgtcaca gcgcagaagc gcatcaccac
     1981 ggcgaagact gcacctgcgg gacatgccgg gacgggcacg gccataacca cgccgaacac
     2041 catcatcaca gccacgaggg gcatcaccac gcggacgagg tgttcacaag ctggggaatg
     2101 gaatcgcccc gcaaattcac cgaggctgag gttgcattct gcctgacggc tctggattcc
     2161 ggcgactacg gcgcggtgct gcgcgccaag ggcatcgtcc ccgcggcgga cgggacctgg
     2221 ctgcattttg actatgttcc cggtgagcag gacatccgca cgggcgccgc cgattacacc
     2281 ggccgcctgt gcgttatcgg cgcacagctc aacgaagcgg cgctcaaaac gctgttcggc
     2341 ggggaggcat gagcatgagt atccctgttt atttgttcac gggctttttg gacgcgggca
     2401 aaaccgcttt cattcaggaa acgctggagg acccgggctt caacaccggc gaaaaaacgc
     2461 tggtgctgct gtgcgaggaa ggtgaaaatg aataccgccc cgaagggttc gcaggcggaa
     2521 acgtcagctt tctgccggtg gaggagcagg ccggcctgac caccgctttt ttaaaagact
     2581 accagaaaaa acaccgtgtg gaccgcgtgc tcattgaata caacggtatg tggccgcttc
     2641 aggcgctgta tgacgcgctg cccacggact gggatatcta ccagatcatc cttctggcgg
     2701 actctaccac cttcgcctcc tatatgacca atatgcgcca gcttgccgtg gacaaattgc
     2761 aggaccctga aatggtgatc ttcaaccgct gcaccgacgc cacggacaag gcgtacctgc
     2821 accgtgccgt tcgcatggtg aaccgccgcg cccagatggc gtttgagcgc accgacggca
     2881 gcgtggaccc cgacgacgtt gtggatgagc tgccctttga taccgacgcg cctgtcatag
     2941 acatcgcgga cgaggatttc gggctgtggt atctggacgc tatggacaac ctggacaaat
     3001 acatgggcaa gaccgtgcgc ttcaagggtt atgtctgtca gacgccccgt gtgcccaagg
     3061 gctgctttgt gcccggccgc ttcggcatga catgctgcgc cgaggatatc tctttcatcg
     3121 gttttatctg cgcggcggaa aacgccgccg cgctgcagca ccgcagctgg gtgaccgtga
     3181 cggccaccgt gggcgcaaag gagcatccca tctatgacgg cgtcggcccc tggctggagg
     3241 ctgccagcgt cacccccgcc cagccgccca aggaagagct tgtatacttc ctgcgctgaa
     3301 ccgaaaacan nnnnnnnnnc ccgccgggtc tccggcgggc ggatgctgtt tattgaacga
     3361 taaaaattcc gctcagttgt ccacgccctt tttgcggaag gccagcgcga acagccctgc
     3421 actgcccgcc gtccacacgg caagcacagc gacgtcccaa agcacgttcg cccccatcca
     3481 cgattggtgc ccaagcccca gaaacattaa atcggccgta gcggtggtcg gcacaaagga
     3541 cgcgactttc tgaagcacgc cgcccatccc ggcaaacatg gtgggcagca tgaataccag
     3601 agccagcggg cctgcaatgg tgcccgtaga ggtctggtcc cggcagacga tccccgtcag
     3661 gccgcccaac agcagcatcg ttatgctgcc tgccgtgcac accagcagga ataccggaag
     3721 ctgcgccggg ccgaagcccg tcagaaaaaa caacaccgtg tccaccagca gagtgcctgc
     3781 cagcaccgcc agcgttttcg cggccagaaa ctccccgccc gtcacattgc acagcatcag
     3841 cgtgcgcatg gtgttctttt ccttttcctc ggcgatcatc atgcccagca cggaaagcgg
     3901 cgtcaggcac aggttcatca tcacgcccag catcaggaga taagcgggcg acatgccaag
     3961 atctacgaac tgatagataa cgataaagat cagcggcagc gccagcatca ggaggacgtt
     4021 gagatttttg aacatatcca caatgtcctt tacaaacagc gtgcgcaaaa cacgcagatg
     4081 ctttttcata ccaaattcct ccccgtcacc tgcaaaaata tttcttcaag gtccggctct
     4141 tccgaatgca cggacgcaat gccccgtccg gaaaggtccg ccagcagcgc cccaatcgcc
     4201 tctggattcc tggcacactc cagcgcctca ccgtcctcca gcaatacgcg caccctgtct
     4261 ttggcatact tcagcttcag ttgtgcgggc acgcctgtct cggcaatacg cccctcgttc
     4321 aaaaaagcca cgcgggcgca caaacggtcc gcctcctcca tgttgtgcgt ggtgaggaag
     4381 atcgtcgtgc cgccgtcgtt catttcccgc agcaggcggt ggatggccgc cgtggtggcg
     4441 gggtccagcg cagcggtggg ctcgtccaga aacagcagcc ggggccggtg cagcacggcc
     4501 atggctaaga tcagcctctg gcgcataccc ttggagagtt tttttgccac gcgcttgccc
     4561 gcgtcggcca ggcccacttt ttccagcact tccccgatgc gggaggcggg caggccgcgc
     4621 aggtctgcaa aaagcttcat attttcatag atgctcatac gctcgtacac gccgctgttg
     4681 tcggacaaaa tgccgatctg ttcataagcc gcgctgtcaa ggcgggcaat gggcgcgccg
     4741 aatacggcaa tctctcccgc gccgggccgg agctgacgtg tgagaagctt gatggtagtg
     4801 gtttttcccg cgcccgaagg cccaagaaaa ccaaaaatct ccccttcctt cacggcaaag
     4861 ctcaggccgt ccagcgcccg ggtactgccg aaggaaaggg atacgttctg catggataga
     4921 atgtccattg gcggttcgct cctttcggtg aatgatatca ttttaccgaa aaaaaagcag
     4981 tcttgctaca aaaatgccgt gaagggagca tcccaaacgg cgaacggagc attacggggc
     5041 atgaacggag cagaaaccat tgccgttttc cgccgccgcc cttttatttc cggcgcgaaa
     5101 attcctccaa aggcccataa agcagatacg ggcatccgta tcgaaacgat aagctgcacc
     5161 atccccgccg catacgccgc ccgggcaggc acagctttta ttttctcccg ccatacgcgg
     5221 cttctttgtc aaaaccggta ctgccgccgc atggcatcaa tgcggccctt gctgagcggc
     5281 acaccggatt ccggcccgtt ctccagccgc agcgcgtagc tgttgcgcgt ccagcgcacc
     5341 acctcctgta ccttctgcat gttcacaagg taggagcggt ggcagcgaaa aaagccgtag
     5401 cgttccagcc ggacagacag ctcctccagc gtgagcgcgc aggcgtattc ttcgccccgg
     5461 acgctgagcg ccgtgcggcc ggacgcgctt tcggcaaagt cgatgtccga gggattgaac
     5521 agcagcaatg tctccccggc tttggccggg atcttttcca ccgctgtgcc ctgcgccgtg
     5581 ctttcctcgg cggcctcaag cctttccaat ccgttcccat ccaaatggta gcgctcgccc
     5641 ggcagcagat agaccgtgcg cgtggaagcc gtggccgcga tgcagcaccg gcctgtggac
     5701 ggcacgccgt ccatccattc cagaatacgc ctgcatcctt ccgcgtcctg cccggccagc
     5761 ggttcttcga tgtaaaacac ctcgggagcg cggacgatct cccgcgcgat ggaaagccgc
     5821 ctgcgctggg ccggcgtcaa acggcgcagc ggtgtgcgcc gcgcgtcctc cagccccatg
     5881 gcggccagcg cggcgtcggc catgccccgt gtgcccgcca tccgctcgaa cagacgcagg
     5941 taagcggccg tatccattct gtcgtaaaag ccttcgtccg cacgcacgaa gccaacgctg
     6001 cgcccgcttc gggccagctc cgcgcgcaaa ggcgcgcaaa gctccggcgc acactcgaag
     6061 ttcacggcac agccgccgcc tttcagcagc agttcccggg ctgcgtgcgg cagcgccgca
     6121 ctgctttcca tgtcagcttt cctccccgat ccgttccaaa aacgctttgc cgtaggcgtc
     6181 ggcctttttg ccgcccacgc ccggcacctt caaaaattca gccatcgtgc gcggcttttg
     6241 ggcgctcatg gcgcgcagcg tcgcgtcggt gaaaataacg aacgcaggca cgcccgcgcg
     6301 ctttgcgaat ttcaaacgca gttccttcag gcgctcgaac agcgccatat ccgcgggcaa
     6361 tgccgccgtg ctctcgtggg cgcggggtgc ctccctggcc acgcgcagcg tgacggtgcc
     6421 gccgtattcc agaaattcca gcgcgggcgc ccccagcgaa agcgtgggat actgccccgc
     6481 tgcctgttcc agataacccg cggccgtaag gtggttgaag atcgcgcgca gccgcgcggc
     6541 ggacacactg cgcaggcggc catagccgtc ggcacgctcc agccggtggc gcagcacgcg
     6601 ctcgctctcg ctgccgcgca gtgcgtcgat gacagtggcc gcgcccagcg ggtagcgcag
     6661 gtccctcagg aacaggaaga tgatgcgggc ctcgccgctc acgtccacct tttccgtctg
     6721 cgtgctgcac accgagcagt tgtcgcagtg ttcgggcgcg ttctcaccga aatagcgcag
     6781 cagatacgcc cgcaggcagc tgccggaatg gcagtaaaat gtcatacggc gcagccgttc
     6841 ctcgtccttc tcccgtacca ggcggcgcgt ttcctcgtcc agcgcgtcgt tttcatcgtc
     6901 cttttcaata aaaaaccggt tcgtcaccac atccttgccg ccatacagca aaatgcagtc
     6961 cgccggctgg ccgtcgcggc ccgcgcgccc ggcctcctgg taatagctct cgatgttttt
     7021 tggcatgttg taatgcagca caaagctgac attggatttg tcgatgccca tgccgaaagc
     7081 gttcgtcgcc accatcaccc gcgcttcgtc atacaaaaac gcgtcctggt tgcgctggcg
     7141 ctcgtccgcc gaaaggcccg cgtgataacg cgtggccgcg taaccgtcgg cccgcagctt
     7201 ttcgcacacc tcttccacgg tcttgcgcgt caggcagtac acaatgcccg aagcgcccgt
     7261 gtgggccgcc agataccgtg tgacggccgc gtatttgtcc cgcggctcct ctacaccaaa
     7321 atacaaattc ggcctgtcga agcccgtcac cacggttaca gggtccgcga ggcccagcag
     7381 gcgcacgata tcctcccgca cctcgcccgt ggccgtggcc gtaaacgccc ccaccacggg
     7441 gcggcggggc agcgtttcca gaaagcgggc gatatccaga tacccggggc ggaaatcctg
     7501 cccccactga gacacgcagt gggcctcgtc cacacacacc atgctgatgt tcatgccctg
     7561 cgcaaaattc tgaaagcgcg gcgtgaacag ccgctcgggc gcgacgtaga tgattttata
     7621 cacaccttcc atggcgttgg ctatggcctt cgccgtctgc ccctccgtca gggaagagtt
     7681 caggtatgca gcgcgcacgc cgctttgcac cagcgcgttc acctggtcct tcatcaggct
     7741 gatgagcggc gacaccacca gcgtgatgcc cggcagcagc agcgccggca actggtagca
     7801 catggatttg cccgcgcccg tgggcatgat gcccatggcg tcgcgcccct gcaaaagcgc
     7861 gtcgatgagc tgctcctgcc cggggcgaaa accctcgtag ccgaaaacag atttgagcaa
     7921 cgcgcgtttt tcactcaccg gttgtgcttc cattccaaaa attcctcgta agtgcccgtg
     7981 cggtccagac agcccggctc gcccagcggc agctcgatga tgcggttcgc gatctcatcg
     8041 atgaactggt ggtcatggga cgagaaaagc aggttgccgg gaaagcgcgc caggccgtcg
     8101 gccagcgcct ggatgctctc caggtccaga tggttggtgg gctggtccag cagcagcaca
     8161 ttggcgtggg tcatcatcat acggctgagc atacagcgta ccttttcgcc gccggacagc
     8221 acctttgcgg gcttgtatac ctcgtcgccg gaaaacagca tccggcccag aaaaccgcgc
     8281 agatatgttt cggtggtgtc ctcgctgtac tggcgcagcc aacagataag gtcctcgtca
     8341 aagccctcga aaaaagcgga attgtcttgg ggaaaatagc tctggctcgt ggaaacgccc
     8401 cacttgaagc tgcccgcgtc cggctccatc tcgcctgtca ggatcttgaa cagcgcggtc
     8461 tggcccgcct cgttttcgcc cacgagggcg attttgtcgc ccttgttggc gataaagctg
     8521 acattgtcca gcaccttcac gccgtccacc gtcttggtga tgccctccac aaacaggatg
     8581 tcctttccgg cctcccgctc cggctcaaag cccacataag ggtatttgcg gctggaggcg
     8641 ggcatctcct ccagcgtaat ggaatccagt atcttcttgc ggcnnnnnnn nnngtcttct
     8701 tgcggcttgt ggcctgcttg gatttggatt tgtttgcgga aaagcgctgg ataaagtttt
     8761 gcagatcgcg gatcttttcc tcttttttct tattctggtt gcggatgagc tgctgcatca
     8821 gctggctgga ttcgtaccaa aaatcgtagt tgcccgcata catctgcacc ttgttgtaat
     8881 cgatatccac gatatgggtg caaaccgcat tcaaaaagtg gcggtcatgg ctgacgacga
     8941 tgatggtgcc gggataatcc atcaaaaatt cttccagcca atcgatggat tcaatgtcca
     9001 ggccgttggt gggctcgtcc agcagcacga tggacggccg gccgaacaga gcctgcgcca
     9061 gcagtacctt caccttctgg cggccgtcca gcgccgacat gggcgcggta tgcaggtcca
     9121 tcggaatgcc caaaccgttc aaaagctggt ccgcctccgt ctcggcctcc cagccgttca
     9181 tctcggcaaa ttcaccctcc agctcagcgg ctttttcacc gtccgcgtcg gtaaagtcct
     9241 ctttcgcata cagtgcttct ttttctttca taatgtcata caggcggcgg ttgcccatga
     9301 tgacggtgtc cataacattg tacgcgtcgt atttgaaatg gtcctgctcc agaacgctca
     9361 tccggtcgtc cgcgcccata gtcaccgagc cggtggtagg ctccagcgcg ccggagagga
     9421 tgcgcagcag cgtggacttg ccggcgccgt tcgcgccgat gatgccatag cagttgccgg
     9481 ccgcaaattt cagatcgacg tgtttataca gcgtgctgcc cgcgaactgc aggctgacgt
     9541 cggttacagt taacataaaa ttctcctttt tcccgggccg caccgcacaa tccgcggctg
     9601 acgccttaaa ctccgtcccg cctgcagctt ttccgtcctg tttcgcaaaa gccctcgcca
     9661 aaacacaacg tgcggcggtt tcgcccacgg gcgaaatcgc gctgaatgga gccgcctgcg
     9721 ccgaaggcac aagctgtgag ccttgtttca tgtgacgaaa aaatttttgt acggtccggc
     9781 gttttggaat gctacagtgc cgccctttta caaaacgcta tcttgtttac cataccatgc
     9841 ccggcacgct ttgtaaatac ctttatactt tattattatg gacgcacggc gcttgacgga
     9901 tgccgccgcc atgccgtaca atggatgctg tacccccgct gcggcagcgc cccgcgcccc
     9961 gggcgggttc agaaaggagc gccgctttat gccgctgatc atcccacaga ccctgcccgc
    10021 ctacaaggcg ctcgaggaag aaaacgtgtt cgtcatgcac cgggcccgcg ccatgacgca
    10081 ggacatccgc ccgctgcgca tcctgctggt gaacctgatg ccgacgaaaa tcgccaccga
    10141 aacacagatc gcccgcatgc tggccaacag ccccctgcag gtggagctga cgctgctgca
    10201 catggattcc cacgagagca cgcatgtgcc cggagcgcat ctggaggcgt tttacaaaac
    10261 cttcgacgag atcaaaaccg agcgcttcga cggcatgatc gttaccggcg cgcccgtgga
    10321 aatgctggcc tacgaggaag tagattactg gcccgagctt tgcgccattt ttgaatacag
    10381 caaaacgcat gtgtacagca cggtacacat ctgctggggc gcgcaggctg cgctgtatta
    10441 ccattacggc atccacaagc gcgtactgcc gcaaaaagtg ttcggcattt tcgcccaccg
    10501 ggtgacacgc ccgaaaaacc cgctggtgcg cggctttgat gaagtgttct acgcgcccca
    10561 ttcccgccat accgagcttt gccgcgagga ggtggacgcc tgccccgcgc tgcgtgtgct
    10621 ggccgaaagc ggcagggccg ggctgcatat cctcgccaca gattccggcc gccagatctt
    10681 tgtgtgcggc catatggaat acgaccgcga caccctgcgc ctggagtacg agcgcgacgt
    10741 taaaagaggc cttcccatcg ccgtaccgga gaattatttt gaggatgacg accccgccaa
    10801 gggcgttgtt ttccgctggc gcagccacgc caacctgttt ttttcgaact ggctgaatta
    10861 ttatgtctat caggaaacgc cttacgattt ggagaagctg tgaaatggaa gcaaagacag
    10921 taaaaagcaa tatgacggat atgacgcagg gcagcccagc cggactgatc ctgtttttcg
    10981 cgctgccgct gctggtgggc aacatgttcc agcagcttta caatatggtg gacagcatcg
    11041 tagtgggccg ctttgtgggc agcacggcgc tggccgccgt gggtacggcg ttccctgtgg
    11101 tgttcctgct gtccagtatg tttatggggc tgggcatcgg agccatggtc atggtgagcc
    11161 agttctacgg cgcgggcgac gccgaacgcc tgaaggccac catcgacact atctataccg
    11221 cgctcatcgt cggcatcgtg ccgctctcca tcgtgggcat ccttgtcagc cgccccatca
    11281 tcgcgctgct ggcggtcccg gccgacaccg cggaccagtg ctggatctac atggtcatcg
    11341 tgctgggcgg gctgatcggc agcctggggt ataatgccaa ctccggcatc cttcagggcc
    11401 tgggcgacag caaaacgccg ctgctgttcc ttgtcatagc gtgcatcatc aacatcgtac
    11461 tggaccttct gttcgtggtg gtattccact ggggggtagc gggcgtggcc attgccacca
    11521 tcattgcaca ggcgttcagc tggatattcg gcattttcta catcaacaaa aaatatcccc
    11581 agatccacat ccatccgttc tgcttcaagt tcgacaaagc gcttttcggc cagatcgtca
    11641 agctgggcgt gcccgccggc gtgcagcagg cgctgttcag cttcggcgtc atggccatgc
    11701 agcggctcat caacagctac ggttcggcgt ttatggcggg cttcaacggc gccaacaggc
    11761 ccaagcgcgt gcacaagggc actgttgcca cgctgtggat gagctgcggc ttcagcgtgc
    11821 tggtggccgc gctgctgctg cccacgggac gcttctgcat gcgtctgttc tccaccgaac
    11881 ccgccgtcat tgaaagcgga tttgcgtacc tggcgcgcat tctgccgttc tactggatgc
    11941 tggccattat gttcgtgctg tccagcatca tgcggggcgc gggcgagatg atggtgccta
    12001 tgatctccag ccttgcggcg ctgtggctgg cccgtgtgcc cgcggcctat ctgctggcgc
    12061 attttttcgg gccgatgaac atccatttct gctacgccat cggctgggcg ctggggctgg
    12121 ccatctgcgt gccgtactat ttctccggcc gctggaaaga aaaaagcatt gtcgtgcgcg
    12181 cggccggcgg aagctgaata caaaaaagcg ccttgaacgg gaccttcaaa agtcctgttc
    12241 agggcgcgct tttttgctgc tgctattttc gacggtctgc ccggacaaaa ggcggctcaa
    12301 cgcgcggctc caccagaagc ctgtattttt cgggacggcg gtatgcattg ccgtgcgcct
    12361 c
//
LOCUS       DAWBSB010000039        12308 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1221_length_12308_cov_3.838325, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000039 DAWBSB010000000
VERSION     DAWBSB010000039.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 12308)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12308)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12308
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1221_length_12308_cov_3.838325"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2596 a         3566 c         4064 g         2082 t
ORIGIN      
        1 gggccgcgac aacaccaaaa tctatttgga ggaacacccc gagctgttcg cggagattga
       61 aaagcaggtg cgcgaaaacg cggataaatt gaacgcggga aaaaagggcg ccgccgcgaa
      121 aaaaaaggcg tccattgacg acgcgcccga tgtggcgaag cccgccgcca aggcggaggc
      181 gcccgctccg gctgcgcccg ccaagcgcgc cagcatcgat attatggtgg aagacgacga
      241 gtgacgatca gccgcattgc aaaaacaaaa caggggcgct tttccttgtt cgatgaaaac
      301 ggggatttcc tgttttccat cgacggagaa accatggtga aaaaccatgt gagggaaggc
      361 gcggcgctgg atgccgcgtc cctggaggcc ctgaaagagc agagcgatac ccgcaaggcc
      421 aaggacaagg ccctcgggta tctttctttg cgcgaccatg catcgggtga gctgtatgac
      481 aagctgtgcc gaaaattcga cgcccgctcg gcggctgcgg ccgtggctga aatgcagcgg
      541 ctgggtttga taaacgatga aaattttgcc cggcacaggg caaaatatct ggcggaccag
      601 aaccgctctc cacgggagat ccggcgcagg ctggcggaga agggcattga ccgggatgcc
      661 gttgacacgg cgctggccga gcttgccccc gacgagacgc aggcctgccg cgcgctcatt
      721 gagaaaaact atgggcgcag gctgcgcgag ggcggacgtg ataaggtcat tgccgcgctg
      781 gcgcgcaggg ggttcagcta cggcgccatc cgggcggcgc tggagcagtg cggtgaaacg
      841 gcggacgagg acggaggctg ggaatgaccc gccgcgcccc ggcggaaacg cccgcgcact
      901 tttgtccgct gtacactttt gatttttaac acgggagacg attatggtaa ttgcactgat
      961 ttccctcggc tgtcccaaaa accaggtgga tgccgacgtg atgtgtcacg ctctgctgga
     1021 gcgcggccat atcaccaccg ccgacctctc cgaggccgac gccatcatcg tgaacacctg
     1081 cggcttcatc gaaagcgcca agcaggaggc catcgacagc attctggagg cctgttccta
     1141 taaggaacag aagccggagc tgaaggtggt cgtaacaggt tgtctggcgc agcgttacca
     1201 gcatgagatc gtcaaagaga tcccggaggt ggacgccgtt gtgggccttg gctccaacgc
     1261 ggccatctgc tccattctgg agcggctgac aggcacgcag cctgtggaga gctacggtgc
     1321 aaaagagagc cttccgctgg gcggcaagcg cgtcatcagc acgccgcgcc attacgcgta
     1381 cttaaaaatc gccgagggct gcaacaaccg ctgccattac tgcgccatcc cgctcatccg
     1441 cggcccgctg cgcagccgta ccatcccgga tattgtggcc gaagcgcgct ttctggcggg
     1501 tgaaggcgta aaagagctca tcgttgtggc gcaggacatc acggccttcg gcgcggaccg
     1561 cggccgggtg gagatcgcgg agcttctgga tgcgctgaac gccgtggagg gcatcgagtg
     1621 gatccgcctg ctgtacgctt atcctgagcg catcaccgac gcgtttatcg cggccatgac
     1681 gcgcaacgaa aaggtgctgc attatctgga cgtgcccatc cagcacatca attccgagat
     1741 gctgcgcgcc atgaaccgcc ggggcgaccg ggagacggtg ctggacgcgc tgacgcgtct
     1801 gcgcgctgcc atgccggaca tcacgctgcg taccacgctc atcacgggat atccgggcga
     1861 gacgcaggcc cagttcgaag aattgtgcga atttgtcaag gattatgcgt tcgaccgctt
     1921 gggctgtttt gcgtacagcg aggaagaggg cacggtggcg gccggtctgc ctcagctgcc
     1981 gcaggagctg cgcgagcagc gcgccgaggc cgtcatgaac atccaggcga acgtgatggc
     2041 gcgcaggcag gaggaaaggg tgggccgcac cgtgcaggtc atctgcgacg gctacgacga
     2101 cgaaaacgac ctgtatatct gccgcacccg cgcagatgcc cccgatattg acgcggaatg
     2161 ctgtgtgcgc agcgaaacac cgctggagcc cggcgcgttt taccgggtga ccattgagga
     2221 gagcgacgtg tacgacctgt acggggcttt ggccgaaggc tgacgccgcc gcggggacac
     2281 ccattgtccg cgcgtcgttc tccggcgcgc cgaaaaggag ttttgaacga tgaacctgcc
     2341 caataaactg acgatgctgc gcgtgctgct gattcccgtg tttatggtgt gcgcggccaa
     2401 ccgctggatg ttggcggccg gcattatttt cgccgctgcg agccttaccg attactttga
     2461 cggccacctg gcgcgcaaaa acaatttggt gacggatttc ggcaaatttg ccgacccgct
     2521 ggccgataag ctgctgacga cgacggcatt cctgtacatg ctggtggacg gtgtgtgcga
     2581 ccccgtggtg ctggcgatca ttctggcccg ggaattcgcg gtctccggcc tgcggatggt
     2641 tgcagcgggc gcgccgggcg gcaaggtcat cgcggccaat atgtggggca aggtgaaaac
     2701 cgcgctgcaa atgctcacca tttgttttta ttactttggt tatgcggcgg aggccgcgtt
     2761 ttccgcgcac ccggggcgca gcgtgtttgc cacgcaggtc atcagctatg tgctgtgctg
     2821 ggtggtggcg gctgtcacgc tcatttccgg ggtaaaatat ctttgggaca accggcattt
     2881 catcaatacg gcaaaataat aatgcgcggc gggatgccgc ccgtgccgaa caggaacggt
     2941 aaaagcggcc gcgcgggagg gttcccgtgc ggccgctttt gagcggaaag ggagcggata
     3001 ccatggcgac acaggcaaag ctttcagatt acggcatttc agtggggacc ctgcccagcg
     3061 ggccgctggg aaagatctcc gatgtaccgg gtgtgacggt gggccactgc accgtcgata
     3121 cacagacgca aaaaacgggt gtgacggtgg tgatgccctg tgcggacgac atgtttgccc
     3181 aaaagctgcc cgccgcggcc tgtgtgctca acggcttcgg caaaaccgcg ggactggtgc
     3241 agatcggcga gctgggcacg ctggaaacac ccatcgcact gacgaacaca ctgaatgtcg
     3301 gcctggtgta cgacgcaatg gtggaatata tggtggggcg ctgtgcccaa agcggcacgg
     3361 cgctgcgcag tgtgaaccct gtggtggcag agtgcaacga cggtgggctg aacgacattg
     3421 cccgccgcgc ggtgcggcag gaacatgtgt ttgcggccat tgaaaacgcg cgggccgatt
     3481 ttgaggaagg cgatgtgggc gcgggcaaag gcatggtgtg ccacggcctg aaaggcggca
     3541 tcggcagcgc cagccgcatt gtggagctgg acggcgcgcg ttataccctg ggcgtgctgg
     3601 cgcttgcaaa ccacggcagg ctggaggatc tgtgcatcgg cgggaaaagc gtcggcgcca
     3661 aaatcgcggc gcatttggaa agcgcgccgg aacagcagca ggacaagggt agctgtattg
     3721 ttgtgatggc gacagacctg cctctctgcg cccggcagct ggagcgcgtg gtaaaacggg
     3781 cgtccgtggg gctggcgcgg ctgggcagct acatcggcca cggcagcggc gaggtgtttt
     3841 taggcttttc cacaacgaac cgcgtgcctc atgaaagcgc ccgcgcggtg ctgccctgca
     3901 cggtgctgca cgagggccgg atagacgcgg tgtttcgggc catgggagag gccacggaag
     3961 aagccgtgct gcgcgccatg ctgtgtgcgg atacggtgac cggctttgat ggcagcacaa
     4021 aatatacgat tcgtaatttt gctagtcaaa tatactagtt ggcacaaact gtttcacaaa
     4081 agagaattgg ccggcggtca aaatcggtta cagcttgata acaggtcttg cataatccgg
     4141 ttttctatgg taatataact aatgtacgat agtaaactta tgaaagagtg ggccgcagag
     4201 gtccgctctt tcctgtttgt ttggaggttt ttgatggcaa aaggcaaagg cggcggcacc
     4261 gtcgggacag tggaggcgct ggtcacgccg gtgctcgagc agctcggcat gcgcctgtgg
     4321 gatgtgcgct ttgaaaagga agggccggac tggttcctgc ggatctatat cgacagagat
     4381 gagccgctgg acctcaacgc ctgtgaggaa gccacgcgcg ccatcaaccc cgtcatcgac
     4441 gcggcggacc ccatcgacca aagctatttt atggaggtgg gcagcccggg cctgggccgc
     4501 aaactcacca gggacgtcca ctttgaggca atgcggggcc gcagggtggc cgcgcatctc
     4561 atccgcgcaa cggccgcggg ggaaaaggac gtggcgggca tcctgcaggg caaggacggc
     4621 gcgcgcgtca cgctgctgct ggacgacggc tccgagacgg ttatcgagga aaaagaagcg
     4681 agctacttca aattgtgcga cgatgaagac ctgttttgac gcataggatg aatgtaaata
     4741 ttatatagaa tggatgattg gaaaaaatga acgcagaatt ttttgaagca ttggccatgc
     4801 tggaaaagga acgcggcctg cccgccgatt acctgctgga aaaaatcaaa aacgccatcg
     4861 tgatcgccgt gaagaaggac tacgaggtcg aggatgaaaa cgtgtccgtc gtcatcgagc
     4921 cggaccaggg caaattcagc gtttcgctgc ttaagaccgt cgtcgaggaa gtggaggacc
     4981 ccgctaccga gatctctctg gaggaggccc agcagaaaaa gaaaagctgc aaggcgggcg
     5041 atgaatacgc catccctctc aagacaaagg acttcgggcg catcgccgcg cagacggcta
     5101 agcacgtcat ccgccaaggc ctcaaggagg ccgagcgcag ccagatgtac gccgagatgc
     5161 agagcaaggc ccatgagatc atctccgccg tcgtcaccaa catcgaaccg gtcaagggca
     5221 ttgtgacgct ggagctgggc aagggcggcg tggccacgct gccccgcaac gagcaggtcg
     5281 ccggtgagga gctgcgcgag ggccagcatg tcaaggtata tgttgtggat gttatggaga
     5341 ccgagcgcgg cccgcgcatg atgatcagcc gcacgcatcc cggccttgtc aagcgcatgt
     5401 tcgagatgga ggtgcctgag atcttcgacg gcaccgtcga gatcaaggcc atcagccgcg
     5461 aggccggcgc ccgcacgaaa atggccgtct ggagcaagga tgaaaacgtg gaccccgtgg
     5521 gcgcgtgcat cggcccccgc ggccagcgcg tggccaacat cgtcgaggag ctgggcggtg
     5581 aaaagatcga catcgtccgc tggagcgagg accctgccca gttcatcagc gcggcgctca
     5641 gccccgccac cgtcgtgggc gtggagttgc tggagggcga taccaaatcc tgccgcgtca
     5701 ccgtgccgga tcaccagctt tcgctggcca tcggcaacaa gggccagaac gcgcgcctgt
     5761 gcgcgcgcct gacgggctat aacatcgaca tccgccccga gagcggttat tatggcgagg
     5821 agccgcccat gaagaaaacg ccggagcccg ccgccgagga ataacggccg gacgggactt
     5881 ggaaaaggag acatgtatga cgccaaaaaa gatacccctt cgccgctgca acggctgcgg
     5941 cgaaagcaaa ccgaaaaagg aactggtgcg cgtggtgcgt gcgggggacg gcacggtttc
     6001 gctggacctg acgggcaaaa agcccgggcg cggcgcgtac gtgtgcccca gcgcggcatg
     6061 tcttgccaaa gcgcgcaaag ccgggcgcat cgcccgcgcg ctggaggtga gcatcccgga
     6121 agaaatttac gacgccatgg ccgccgagat cgagaaggcc ggggcgcaaa aaggagagac
     6181 gcatgccggg taatatcctg ttcgcacttt ctctttgccg caaggcgggg gccctggtga
     6241 tgggcttcga cgccgtaaaa gacagcgtcg gcaaagggaa ggcacaactg gtgctgtgcg
     6301 ccggagacct ttcggagggc acgcgccgcc gcgtcggcgc cttctgtgag gattgggtgg
     6361 aggtggcgga cctgcccgaa acccagttcg ccctcgcaca gatcagcaaa aaacccaccg
     6421 gcgtattcgc cgtaacagac cccgagcttg caaagctctg ccgcaaaaac cttgctgccc
     6481 aagcggccaa ccagaaggag gaacgtccat gtcagtagtc atcaaataca aactcagcga
     6541 tgtagcaaag gacctcaatg tccagaataa ggagctcatc gattttctgt atcagacgct
     6601 gggcgagtcg aaaaagcata cctcgcccct tacagaagcc gagctgaacc atgtgttcga
     6661 gcattacacg cagaaaaatt ccgaggcaag cctcgacgcg taccttgcca gcgcgcccgc
     6721 gcccaaggtg gcggaaaagg atatcctgaa aaaggccgac ggcaccgttg tggcggccgg
     6781 cccggccaag cgcaaagagc gcatccgccc cgccaagccc gagaaggaga acaagcccgc
     6841 gcctgagaag cgggaggtcg tcaccgtcac ggtggatacc cgcatcacgg acgtgaatct
     6901 ggataagttc aacgaaaaat acaccgagct ggcttccacg aaaaatgtgg agaaccgccg
     6961 caagcccacg cccgcgggca acaaacaaaa gttctcgggc cgcaacaacc gccgccagca
     7021 gccgttccag aaaaagcgcg agacagaggc cgagcgcctg cagcgcatcc agctggaaaa
     7081 ggctcgtaag gcgcagctga aggtatccat tcccgacgag atcacggtgg gtgagcttgc
     7141 cagccgcctg aaggtgaacg tggcgcaggt catcaaaaag ctgatgggcc tgggcatcat
     7201 ggcagccatc tccgaggtcg tcgatttcga caccgccagc ctggtggccg aggagctggg
     7261 cgcgaaggta gagcacgagg tgcatgttac catcgaggaa cgcctgttcg aggtggatga
     7321 ggataaggac gaggatctgg aagagcgccc gcctgtcgtc gtggtcatgg gccacgtcga
     7381 ccacggcaag acctccatcc tggacgccat ccgcaaaacg cgcgttaccg cgggcgaggc
     7441 cggcggcatc acgcagcata tcggcgcgta tcaggtgacg atgaacggca agcccatcac
     7501 attcctggac actccgggcc atgaggcttt cacatccatg cgcgcccgcg gcgcgaacct
     7561 gacggacatc gccgtgctgg tggtggccgc ggacgacggc atcatgccgc agaccgtcga
     7621 atcgatcaat cacgccaaag cggcgaatac taccatcatc gtggccatca acaagatgga
     7681 taaacctacg gccaacccca accatgtaat ggagcagctc acgcagtatg agctggtgcc
     7741 tgaagaatgg ggcggcgaca ccatctgcgt gcctgtgtcc gcgctgacgg gccagggcat
     7801 cgaggacctg ctggaaagca tcaacctggt ggccgaggtc aaagaactga aagcgaaccc
     7861 ccaccgccgc gccaagggcg ctgttatcga ggcgcgtctg gacaaggggc agggccccgt
     7921 ggccacgatc ctcgtccaga agggcacgct gcatcagggc gatgtggtca tcgcgggtac
     7981 gtccgtgggc cgtgtgcgcg tcatgcgcga cgaccagggc gccatcctca aggcggccgg
     8041 tccctccatg ccggtggaga tcacgggcct gacggaggtg cctgcggcgg gcgatctgtt
     8101 cgacgccgta gaagacgaga agctggcccg tgagctggcc gaccgccgtg ctgccgagca
     8161 gaaggaacag cagttcagcg catacaagaa ggtcacgctg gataacctgt tcagccagat
     8221 cgccgagggc gaacgcaagg agcttgccat cattgtcaag gccgacgtgc agggcagcgc
     8281 cgaggccgta aaacagagcc ttgaaaagat ctccaacgac gaggtgcgcg tgcgcgtcat
     8341 ccatgcgggc gtaggcgcca tcaacaaatc cgacgtcatg cttgccaacg cgggcaatgc
     8401 catcatcatc ggcttcaatg tccgtccgga ccccgttgcc aaggaggaag ccgcccaggc
     8461 cgaggtggag ctgcgcatgt accgcgtcat ctacgacgcc atcaacgatg tcaccgacgc
     8521 catgaagggc atgctggcgc ccaagttccg cgaggtggag ctgggccgcg ccgaggtgcg
     8581 cagcgtgttc aagatatctt ccgcgggcac cattgccggc tgctacgtgc tggagggcaa
     8641 ggtaacgcgt gccgcgaaga tccgactggt gcgcgacggc atcgtcatca ccgaggacga
     8701 gatcgacagc ctgcgccgct tcaaggacga cgtgaaggaa gttgcccagg gctacgagtg
     8761 cggtatcggc cttgacaagt tctccgacat caaggagggc gatatcttcg aggccttcat
     8821 catggaggaa taccgcgact gaacggaacg ttccgtttga aaggagacac tatgccgcag
     8881 agcaaaaata tggcgcgcct gtccgaggac atgaagcgcg agctgatcgc catcatcggg
     8941 gggatgaaag acccccgcgt gcagggcttc ctgacggtga tgcgcgtgga ggtcgcgccg
     9001 gatctgtcca cggccaaggt gcacgtgagc aagatgggcg aggaaaacgc aacggccgaa
     9061 gccgtgggcg cgctgaaccg cgccgcgggc catgtgcgca gcgagatcgc aaagcgcatg
     9121 cacatccgca aggcgccgga attccgcttc gtggcggacg acggcgcgaa gtatgcggcg
     9181 cacatcaatg aattgatcgc ggggctggag cgggaagagc gctgacgcgc tcggcggggc
     9241 tatccccaaa agcgatgaat gaggagagtt tatgagcgag attttagaca tcgcggcagc
     9301 gctgagcagg ctgctgaaag cggaaaacgt gctgctgatc tgtcataaaa acccggacgg
     9361 cgacaccatc ggcagcgccg ccgcgctgta ctgggcgctg aagggcctgg gcaaaacagt
     9421 ggccgtgctg tgcgcggacc ccatcccgtc ccggtatgat tatatggaca tcggcctgta
     9481 cacaggccag ttcacgcccg gctacacggt agccgtggac gtggcgggca tccagctgtt
     9541 cggcgattcc atcgcggaat atacacgccg catcgacatg tgcatcgacc accatccctc
     9601 caactcaggg tatgccgact gcatgctgct ggacggcgac gccgccgcca cagccgagat
     9661 catttacgag ctgctgacgg ccatcggcac cgagatcacg cccaccatcg cggactgcct
     9721 gtatacgggc ctgtccacgg ataccggctg cttcaagttt gccaatacca cggcccgcac
     9781 ccacgagatc gcggcaaagc tcatcaccgc gggcgcaagc ctcgttaagc tcaacaacat
     9841 cctgttcgag agcaaatccc gcagccgtct ggccattgag cgcctggcgc tggaaagcct
     9901 ggaatacagc tttggcggcc ggtgcgccac ggtctacctt acaaaagagc agatcgccga
     9961 gaccggcgcc gacggcaccg atttggaggg tatcaccagc ctgccccgca tgatcgaggg
    10021 cgtggaggtc ggcatcacca tccgccagca gcccacgggc agctataagg tgagcgtgcg
    10081 caccgtgaca ggcgtggacg cttccgccat ctgcgcccat ctgggcggcg gaggccacaa
    10141 gcaggccgcc ggatgcgaac tgctgggcag tctggacaac gccaaggccg ccctgctgac
    10201 cgaggtggaa aaagcgttat gcaaggaatc ctagttgtcg acaaaccgga gggctttacc
    10261 tcctttgacg tagtggcaaa gctgcgcggc atctgcggta cgcggcgcat tgggcacggc
    10321 ggaacgctgg acccgatggc cacgggcgtg ctgcccgttt ttgtgggcgg cgcggccaaa
    10381 gccgtggata tgcaggagcg tgccgacaaa atgtacacgg ccacgatgct gctgggccgg
    10441 cgcaccgaca ccggcgacgt taccggcgaa acgctggagc aggcggctgt tcccgcggac
    10501 atcggcctgg acgccgtggc cgcggtgctg ccgcgcttca cgggtacgca gcagcaggtg
    10561 ccccccatgt actccgccgt gaaagtaaac ggacaaccgc tgtacaaggc ggcccgcgcg
    10621 gggaagactg tggagcgcac gccgcgcccc gtcacgctgc acagcattat gctggcgggc
    10681 agccccgcac ccaacgagta tattttaaac atccactgca gcaagggcac ctatgtgcgc
    10741 gtgctgctgg aggatattgg ccgggcgttg ggcgtgcccg ccaccatggc ggcgctgcgc
    10801 cgcacggcgt cgggccccta tacgctggaa atggcgtata cgctggatga gctgcagcgc
    10861 gcccgggagg ccggtacgct ggagagcctg ctgctgagcg tggaaagcgt tttcacctgc
    10921 ctgcccgccg tagaggtgaa cggggagacg cgccgccgcc tgctgaacgg tgcgcctact
    10981 taccggttcc cggcccggga cgggcgctac cgcgcgctgt gcgcggggga atttctgggg
    11041 gtgtgccggg tggaaaagca catcctgact gtggaaaaac tgttttgcga acgcgcgtga
    11101 cgcgccccgc cggggccgct gcgcgcggcg ttcgcttttg gggcctttta tcccgagcag
    11161 ggaacggccc gcattgaaaa acgggaggtg tgcgtttgca ctgctacgat actcttgcga
    11221 cgatagaggg cggcacgggc gcctgtgtgg ctcttggcta ttttgacggc gtgcacctgg
    11281 ggcaccgccg tgtgctggcg cgcacggtgg aagctgcgcg ggagcggggc tgcacgtccg
    11341 ccgtgttcac ctttgcaccg ccgctggacc gggctgtaaa aggcagggcc atccttacgc
    11401 cggaggaaaa ggcgcggcgc attgccgcca tgggcgtgga gcattatgtg cgtccgccct
    11461 ttgaggaatt ctgcacgctt tcgccggagg cgttcgtcca cgacgtgctg gctgtgcgtt
    11521 tcggcgcgaa ggctgttttc tgcggggata acttcacgtt cggcagccgc aaggcgggca
    11581 atgtggacat gctgcgccgt ctgtgcgcgg ccgcgggaat ttcggtggag atcgtgccca
    11641 tggtacagcg ggagggcgcg cctgtttcat cgtcccgcat ccgcgcctgc ctggaggcgg
    11701 gcgatgtgga aactgcgggc gccatgctgg gggagcctta ctgcatcgac cttcccgtac
    11761 gtcacggcaa gcggctgggg actgcgttgg gctttcccac catcaaccag atctatcccg
    11821 attggatgct gcttccccgg cagggcgtat atgtaacgcg ggtcctgctg gacggcgtgt
    11881 ggcggcccag tgctacgggc ttgggcacgc gccccacggt gaacggcgac ggcgttacct
    11941 gcgagacgtt tatcccggat ttttccggcg atatctacgg cgacagagtg ccggtgcgct
    12001 tctgccgttt tttgtggccc acgcagaaat tcggtacgct ggaggagctt tcggccatgg
    12061 tgggccgcgc cgcgcaggcc gcgcgggagt ggcgggacga ataagccttt tgctgttata
    12121 agccgcacaa aacttggcaa aacgccttgt ttttgcgtgt gcgttgtgtt atactgttta
    12181 ggctgcctta ttatgggctg caagcctacc caggcccggt gtccggcgca tccctcgatg
    12241 gtctaccgac actgcgtctg cggcaaatca taaaaaagag gagaaagaaa ccatggaaaa
    12301 caaacagg
//
LOCUS       DAWBSB010000040        12196 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1236_length_12196_cov_3.286138, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000040 DAWBSB010000000
VERSION     DAWBSB010000040.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 12196)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12196)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12196
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1236_length_12196_cov_3.286138"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2086..2095
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6359..6368
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7963..7972
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2678 a         3849 c         3326 g         2313 t
ORIGIN      
        1 ccgggcggct ccgccagcag cagcccctac accgagacct gcgaggttaa gctggtaaac
       61 accgaaaacg gctggcgctt tgccggcttt gcaatggcct attgacaggc cgcagcgaaa
      121 agaggaggat gtatatgcgt acagagcgcg aaatgacggc gctcattttg ggcgtagccg
      181 aacgggatgc gcgcgtacgc gctgttttta tgaacggctc gcgcgccaac cccaacgcgc
      241 cccgggatat ttttcaggac tacgacatcg tgtatgtagt ggaggaaacc gcatccttcc
      301 gggcgcagcc gggctgggtc gacgtgtttg gggagcggct cgtcatgcag acgcccgacg
      361 agatggacca tgcggcggga cagcccacag accttgcgcg ctgttatggt tatctgatgc
      421 agttcgcgga cggaaaccgc atcgacctcc ggctgatgac gctcccccgt gcgctggaag
      481 aatgccaaag cgattcccag accattgttc tgctggacaa ggacggcatt ctgccgcccc
      541 tgccgctgcc ctcggacacg gcctaccaca tccgcaggcc ggaccagact gcgttcgcgg
      601 gctgctgcaa cgagttctgg tggacgcttc cctatgtggc caagggcctg tggcgcggcc
      661 gcgtcactta tgcgctggac accctgaatg cctgcgtccg gccccagctt ctgcacatgc
      721 tgtcctggct ggccggcacg cgcacgggct ttgccgtcag cgcaggcaaa agcggtgcgg
      781 acctgcccgc ttacctgccc gccagctgtt gggagcgtta cctttccacc tatgcggaca
      841 cagaacccgg ccatgtgtgg gccgcggtat ttgcagcggc caccctgttt ttggacgcgg
      901 cgcatcagac tgccgaggcg ctggctctcc cggtcaacga agcggaggcc gaaggaagcc
      961 tgcgttatct gtaccgggtg cgtgagctgc ccgcggatgc ttcagaaatt ttttgagcag
     1021 ctgtttccac cgaccgcatc tgtttttatt ccgttcagaa aaggcgcggc tctgaaaatg
     1081 aaccgctcca gtaaaaccgt acaatagaca aaaggccccc taatgtagga aaataaagct
     1141 acattagggg gtcttactat gtccgggaaa agcggagttg cagctccaca gcgaccaggg
     1201 gttccagtac acctcgcaag catacttcaa cctgacaaaa tcttatggca ttacgccctc
     1261 catgtcaaga cgagcacccc catatgataa cgctatggca gaaaatttct tttccattct
     1321 caagacagag tgtatctacc gccataaacc ggcctcattc tgcgaggcca atgagatgat
     1381 tgaccgctac atcaattttt acagctacga gcgcatccag ttaaaaactg gagtggcgcc
     1441 gctcacgctg cgccactccg cttaatactt catatttccc tacatagagg ccttttggtg
     1501 ctgtccgcac aatctggggc ggtttaaaat gagccgcacc ttttccgttt tatttatgcc
     1561 cttttctttt tgccccggcc cagcagctgc tgcaccgtgc tccacaaatc acggaaattg
     1621 atggctgcga tgagcaccac aaccagtgaa caccacaccg gccaaagttt tacctgccac
     1681 atcatcagcg ccgattccag caccagcagg ccgaagttgc agcacagctt ttcgggtgcg
     1741 aaatccacgc ggatgagccc gcgcgtgttc actgcccgca gcaaaaacac gacaaaatag
     1801 ctgacgaacg tggccagcgc tgcgccgttg acgccccaga cggggatgag tgtgaagttg
     1861 agcgcaacgt tgatgcccgc gcccacagcc atggtggtga acgaaagcgt cgagcgcttt
     1921 tcaaccatat aaatactgtt taaaaaattg tcgaagctgg caaatgccgt tgccatcgtc
     1981 aaaagcggga tgaatttcca gccgataaaa tagtcgtcgc gataaatggc cataaagggc
     2041 tgcgccatca gcacgatgcc tgccgccgcc agaaaaatca ggctcnnnnn nnnnngatct
     2101 gccacgcttc ggtaaaaata gtggaaacga tggacaggat ggtgggcagc ttataactgg
     2161 cccccagcac cgccgtccag gaagtgccgt cctccagcat ggcggccacg aaaaactggt
     2221 cgctggcgtt ggtgatccac cagaagatgg acgcaggcac caacggcagc gcatagcgca
     2281 gcattttttt cagcagcttg gtatcaaagc gcttgaaatc cagaaaacgc caggtctttg
     2341 tcactgcaaa caggaacagc gcactgagac catcggcgca gatgatcgca agcaaatagc
     2401 ctgtggggcc catattcagc acccgcagaa acagcacata gaacccgcat gtggttatgg
     2461 tacacagaat accgtcaatg gcatacaggc ggttgagctg gcgtgcacgg cagaactggc
     2521 agttcagcgt gcgcaggcag ctcaccagca ggtacacata caacagagca atatagtccc
     2581 ggaacagtgc aatacgggac agcagcggat acaacagcag cagcagtacg aaccccatac
     2641 caatggatac aaggccgttc atataaatgg cgctcttgcg gatgcccttt tccaacccaa
     2701 aacggataac ggcgttggcg atacccaggc tcaccagcgg gatcagcagg ctggcgcact
     2761 ggttcagcag ctccttgatg ccgttcattt cctgcgtttc aaaccagtag ctgaacagcg
     2821 gagccatcac aaagctcagc agcttggacg aaaacgtact gatcgcaaac aacatcgtgt
     2881 ttgaaacaag gcgcttgtat ttatccatgc agcacctccg gcttccggaa aatttttact
     2941 ctaactataa tagtatatca tatatcctgc cattttaaaa ccacggccct gtgccggcag
     3001 aaccaaaact gcgaacaaag cgccgttttt cctttttaat atgtaggcgg cggtccctcc
     3061 gggctttcca ttccgccttg cacagacggc gccagcagcc tgcggcgcgc cttatagtcc
     3121 ggcatgtacc gcgctaccac agcccaaaaa gcggggctgt ggtctgcccg gacaaaatgt
     3181 gcatattcgt gcagcaccac atattcgaca gccgcgcgag gcttttccgc caggcgcaga
     3241 ctgaacgtga tctgccgttt tgccggcaca cacacgcccc agcgcgtttt catctgccgt
     3301 accttcagaa cggggcgctg cccgttcagc acctgtgcaa acaacggaaa taccgcatcg
     3361 cttacctgtg tgaacagagc cagcgcatcc tccctggaca cggaacaggg gcgctgctct
     3421 tcctcgccgc gcgcaagagc gcgcacgcgc gccgcctcga tccatcgagc ccgtccggcc
     3481 acgaatgcat ccacctgtgc cagcggcgcg cccaggggcg ccgacacggc aacgcttccg
     3541 tcagcccgca cgcgcaggtt gatatttttg acccgttttc gcttaaactc ataccgcaca
     3601 ccgcctgcgg cgcgatactg cacgggttct ttgtttttca tccggccggc tcctcctgca
     3661 gtttccccgc cgccgcccgc aggggcggcg gctttttatg cttccgtgtt ccagggcctt
     3721 tgcgcacaag ggccctggaa cattataccc ccacacaaat gcaattggca aatccgccaa
     3781 aaatgccgcg gaaatttggc atggattctg cttgacatga tactatatct agtgcgataa
     3841 ttatacccgg catcaatatt tagcgagcat accaaagagt gtgagtgtgc gcggaatcgg
     3901 aggaaaacca tgatcaaaag catcatcaag cgcgatggac gcgtcgtttt gtacgacgaa
     3961 ggaaagatcg cttcggccat tttgaaggcc atggaggcag cccaggaggg cgacgcttca
     4021 caggctgcgg ctgtcgccaa tgcagtggaa gcggctctgg aggagcgttg cggcgcccag
     4081 ccgccccaaa tcgagcagat ccaggacgaa gtggaaagcc agctgatgca gatgggcttc
     4141 gacaacgccg ccaaaaaata tattttgtac cgcgcgtccc gcacccgcgt gcgcgagatg
     4201 aacacccgtt tgatgaaagt gttcgacgag ctgacccatg ccgacgccac ggccagcgac
     4261 atgaagcgca gcaacgccaa catcgacggc gacacgccca tgggcaccat gcttcagtac
     4321 ggcagcgagg cagccaagga ctattatgac aaatacctgc tgacacccga gcagagccgg
     4381 gcgcaccggg agggctggat ccatatccat gactttgatt tttacgccct caccaccacc
     4441 tgctgccaaa tcgacctgct caagctgttc aaaggcggtt tctccacggg ccacggtttt
     4501 ctgcgcgagc ccaacgacat ccagagttat tccgccctgg catgtatcgc catccagtcc
     4561 aatcagaacg accagcacgg cggccagagt atcgtaaact ttgactacgg cctggcgccg
     4621 ggcgttgcca aaacatacaa aaagcagtat gccgtcaata ttttcaagag tctcgagctg
     4681 ctggctcccg aggcgggcgt gacgctgcag caggtgaagg acacactgcg cgccattgag
     4741 gctgaacagg gcctgcggcc ccagctcgcc actgatatgg attacctgcg cgccgagacg
     4801 gaagccctca ctccgctcgt gggcggagac attgcaaaaa aagcccaggc attcgcgctg
     4861 aaagagacgg aaaaggagac cgaaaaggct acctatcagg ccatggaggc gctcattcac
     4921 aacctgaata ccatgcattc ccgcgccggt gcgcagacgc cgttctcgtc catcaactac
     4981 ggcacagaca tcagccccga gggccgtatg gtgatgcgca acgtactgct ggccacggac
     5041 gcgggcctgg gccacggtga aacgcctatc ttccccattc agatcttccg cgtaaaggaa
     5101 ggcgtcaact acaaccccgg cgaccccaac tatgatttgt tccagctttc ctgcaaggtg
     5161 agcgccaagc ggctgttccc caacttcagc tttatcgaca gcccgttcaa cctgcagtat
     5221 tacaagcccg gccatcctga gaccgaggtc tcgtacatgg gctgccgcac ccgtgtgatg
     5281 ggcaacgtct acgatccctc acaggagatc gccaacggcc gcggcaatct cagcttcacc
     5341 tccatcaacc ttccgcgcct ggccattgag agccacggcg atgaagcgct gttctatgaa
     5401 aagctcaagg atatgatgga gcttgtcatc ggccagctgg acgatcgttt cgccatccag
     5461 agcaaaaagc gcgtatacaa cttcccgttc ctgatgggcc agggtgtctg gctggacagc
     5521 gaaaagctgg gccccagcga cgaggtgggc gaggtactca agcacggaac attgagcatc
     5581 ggcttcatcg gcctggccga gacgctgatc tctctgtacg gacaccacca cggcgagagc
     5641 gacgcaatgc aggaaaaagg ccttgccatc gtccagtaca tgcgccggta tctggatgcg
     5701 cgcagcgaac gcacccatat gaactacagc ttgctggcca ccccggcgga gggtctttcc
     5761 ggccgttttg tacgcatgga caaaaagcgc tacgggctga ttcccggcgt gacggacaag
     5821 gattattaca ccaattcttt ccatatccct gtgtactaca atatctcggc ctatgagaag
     5881 atccggaagg aagcgcccta tcatgcgctg accaacgcgg gccatatcag ctatgtggag
     5941 ctggacggcg acaccgccaa caacctggaa gcgttcgaga gtatcgtgcg ctgcatgcac
     6001 gactgcggca tcggctacgg cagcatcaat cacccggtgg accgcgaccc catctgcggg
     6061 tatgtgggcg tcatcgggga ggtatgtccc cgctgcggcc gccgcgaggg cgaggccgtt
     6121 tctcccgaac gcatcaagga gctgcgcaag ctgtatcccg gcacctttac ctactgcggc
     6181 tgcgaatgat ctcatccgcg ccgaagcaat agcacaattt ttcaacatct tttcgtcttt
     6241 tgaaggagga cttgacttat ggcacagatc gaaaccaaca ttgaggcaaa aaacggcaag
     6301 ggtgtgggct ttgagcgcat ccgccgcatc accggctacc ttgtgggcac tctggaccnn
     6361 nnnnnnnncc aagcgtgccg aagagcacga ccgtgtgaag cacggcatgc agcattaagg
     6421 agcgcttttg ttttgataca gcaaacgatc aatatcgcag gcatcatgga ggattccatt
     6481 gtagacggcc cgggcctgcg cacagctatt tttgcgcagg gctgcccgcg ccggtgcgag
     6541 ggctgccaca acccggaaag ctgggcgttc ggcacgggca cggatatgac agtgcaggac
     6601 ctgttctggc gtgtgaagaa aaatccgctg gtgcgcggcg tcaccttttc cggcggcgag
     6661 ccgttcagcc aggcggagcc ttttactgaa ctggcaaagc tgctgaaggc caatggttac
     6721 gaggttgcca gctatacagg ctataccttt gaagaactcc tggaaaacgg aacgccggcc
     6781 cagaagcagc tgctggccgc gctggacatt ctggtggacg gagaattcgt tttggcacag
     6841 cgcaatctgg acctgcggtt tcgcggcagc gaaaaccagc gcatcctgga cgtaccccag
     6901 agccttgccc agggcagagc cgtatggtgt acggcggcgc gctgggtggg agaacggcag
     6961 gcataagcgt gcactgcgtc cccgcggcac aaccgaaact ttagccgcgc ccatctcatg
     7021 cgggcgcggc tctgcttgtc gaaaaagggc aattttgtgt gcgaagtgcg caaaattgcc
     7081 cttttgtgcg ccggaaagga gcgctgaggg aaaccggaaa gcgtctgcac agcacaaagc
     7141 gtctggcgtc tctcagcggt tgacggcttt gccggcagcc tcagccgcat tgcgcgtaaa
     7201 gatacttttt gctataatag gtacgttcat cacaagacca gtaaaggaat gaagaccact
     7261 catgcgcatg cgtttcaaac cctatgccgg gccggagctt gcggcctgcc cttttcacat
     7321 gaacgacgcc acccggtaca agggccgctg gcgagagacc tttgcccggc ccgggcagcc
     7381 gcttcacttg gagctgggct gcggcaaggg cggatttctc gcccggcttg cctcccgcaa
     7441 tccggacgtc aattacatcg gcgtggacat caccgacaag gtgctcattc ttgccaaacg
     7501 caacatcgaa cgcatctacg ccgaacaggg gcttcccatc gacaatgtga agaccctggc
     7561 tcacgacatc gaacgtattc ccacactgtt ggacaaagcg gaccgcgtgg agcgcatcta
     7621 catcaatttc tgcaacccat ggaacaaaaa ggccaaccac aaaaagcacc gtctgacgca
     7681 tacccgccag cttctgctgt accgtccgtt ccttgcagac ggcgcggaga tctggttcaa
     7741 atgtgacgac gacgatcttt ttaaagatac gctgcgctat atggccgagg ccgggttcga
     7801 ggtgacctgg cagacgaggg acctgcacgc tgatgaaccg tcgtggaaca tccgtaccga
     7861 acacgaacag atgttcacgg aaatgggcat ccccaccaag gcgctcactg cgcgcaaaac
     7921 gtcgctgccg gaaaacgcgc ccgcgccgtg catcccgcac gannnnnnnn nntggccggg
     7981 agaaggtggg tttgctcgtt tcatcacaaa agcttacgac agcagccgga ccccgaggat
     8041 gggaacgcga agctaccagc ccatccggcg gcagaagcgc atcagattat ccatcgtcag
     8101 tgcaaggtca cttttgctgc ggggctttgc ttcccggaag ggtacggcca cacggttgat
     8161 gctgaacacc gccgacacac ccatatcgta agccgcttca atgttgtctc caatgtcgcc
     8221 caccacggcc accagcggaa cgcccgcgcg ctttgtccgc cgcgccacgc cgatgaccac
     8281 cttgcccagc agcgactggg catccagctt cccttccccg gagaacacca tatccgcccc
     8341 ctccagcagc ttgtcaaagc ccacggtatc cagcacggtc tcaatgccca tttgcagcgt
     8401 gctgccgaaa aaggccacca tgccgccgcc catgccgcct gccgcgcccg caccatggag
     8461 ctgccggatg tctcgcccgg tacagcgctc cacaacatcc gccaagtgcg ccaggcccgc
     8521 gtccagaagc tccaccatgg ccgggtccgc gcctttctgg ggcgcgaaca catgcgccgc
     8581 gcccagcggg ccgcacagtg ggttgtcgat atcgcacatg gtcaccagct ccgcgctcgc
     8641 aagtgcggga tgcaggccgg aaaggtcgat ctccgcaacc tccgccagcg ttgcccccac
     8701 aggcacaaag gcctcgcctg ccgcgttgcg gaacactgca cccagcgctg ccgcagcgcc
     8761 gcagcctccg tcgtttgtgg cgctgcctcc cagccccacc accagccgtt tgcagccgcg
     8821 ctccaacgca tgggccatca gttcacccac gccgtaggta gtagtctgtt cggcgtgcag
     8881 gcgctcgccc accagcggca ggcccgccgc cgccgccatc tcgatgacgg ccacaccgtc
     8941 cggcagcatg ccgtagaacg cgggcacttc ctcgaagtac gggcctttta cagtcacgga
     9001 gaccttttcc ccgcccacgg cctgcagaaa cgaatccaca cttccttcgc cgccgtctgc
     9061 cacaggcacg ctgtgcacct gggcatccgg ataatattcc tgcaccgcct gcgccattac
     9121 ggtacagatc tcggccgagg acatggttcc cttgaaggaa tcggggatga ggatcacttt
     9181 tttcatgatt tacgctcctt gtcaaaaaag tccgggtttc agctttacag gagccggggc
     9241 cctgccccgg ctcctgcacg gtcgggtaag acattcggca cggcgctttt ttgacgctga
     9301 aaacgccgtt accggaaaat aagagagagc acgaacacgc cggccatcga cgaaagcccc
     9361 agcagcagcg tgcacagcgt ctgcgtttta tagccctgct ccggcttcat ttcgccgaag
     9421 ttcgtcacca cccagaaata ggaatcgttg gcgtgggata ccgtcatcgc gcccgcggca
     9481 atggccatca ccgtcagcgc ggcgcgcacg ggcgtgccca gtcccatcgc atccatcagc
     9541 ggagccatga tgcccgccgt ggtggtaagc gctaccgtgg aggacccctg cgccgtcttt
     9601 aggatggcgg acagcaggaa cgggaagaag atgcccacgg cctccagaat agacgcgttg
     9661 gccgtaatgg tctccaccat gccggacacc gcgatcactt ttcccagcac tccgcccgct
     9721 gccgtgacga acaggatggg gccgacggtc ttaagcgtct cgttggtgag ctcgtagaat
     9781 ttgttcagcc gtttcgcacc cgcaagcagt actacaccga acagcgtgcc caccgccagc
     9841 gcgatgatgg gtgcgcccaa aaatgcacac agatcatagg caaagcccgt ccagcccgcc
     9901 atggccgcga tggagcccag cgccatcagc aggatgggca cgacaatggg cgccagcgcc
     9961 gccgccccgc cgggcagctt gccgaactcc gccaccagtt cctcgtatgt cctggccgtt
    10021 tcgccgtcgc atgcctcatc gcccgctttt acctttttgc cgatgtattt tgcgaagaag
    10081 tagcccacaa acaggggaat gatggagcac agcgcgccca tgcccataac gagcagcagg
    10141 ttgtcgccga tgcccagcgt gctggccgcg gcaatgggcc ccggcgtagg cggaatgaat
    10201 acgtgagaga tatacagccc ggccgaaagc gccactgtca tggccacgct ggacgtcatt
    10261 gtgcgcttta ccagcgcctt gcggatgggg ttgaggataa caaaccctga atcacagaac
    10321 acggggatgg aaaccaccca gcccatgagc agcacagcca gctccggccg ttttttcccc
    10381 accacacgga tgaccatatc cgccagcttc aatgccgcgc ctgtggcctc caggatcgtg
    10441 ccgatgagcg cacccaggat gatgacaatg ccgatgctgg tgaacgtgcc ggaaaaaccc
    10501 gcgccgatga cgttggcaag ccccggcgta acagcgccgt ccgcggctgt cctgttcacc
    10561 agcggcacgc ccgccacaag ccccagcacc agcgagatgc ccatgatgga aaggaatggg
    10621 tggatcttcc atttcgagat ggccaggatc ataaggacaa tggccgccac gaacgcaagg
    10681 ataagcggaa aacctgacat gatgaaacct ccttttgaat cggctttgcg cacccgctgc
    10741 tgccgggtgc cgcctgttga tacagtgtac cgggccgcac gctgttttat gatgttttgc
    10801 gcccgtcttt caagccgtat ttcaagcgag atctctgttc ctcatgccaa taccgcgcaa
    10861 ggccgcgcga cagatttggt ttaattgtcc agtttcagct tcccatcaaa agcaggctgc
    10921 cttccacttc cttgaaaaaa agcactgcca tatagaatac cggtgcttgg ctgggccggc
    10981 gcacatcgca gcccgtcagc tcctccagcc gcttcaaacg gtactgcaga gtgtttttgt
    11041 ggatatacaa tgcatccgcc gctgcctgga tggaaccctc cgccgcaaag tatgcctcca
    11101 gaagtccaat ccatcggcgc agctcctcgt aagtgcagcc ccgaaatacc ttacgcagat
    11161 attcttcctt ggtctggcgc ggcacatcgc ctgtgaacag ctccagcgtc acactgtcgt
    11221 aaggcagcac gccttccggc gccatgcgag ccgaacgcca cgccttattt gcctgggcat
    11281 aagcccggtg cacatccggc gcgccgccgt cgatccccac tgccagccgc agaccaaagc
    11341 gttcccgcgc catgacgcac aaacgctgtg ccatggcctg catctgagcg tccggccgcg
    11401 cacgcagcag cagaatctgc cgcgctgcat tccgcagaat gataccgccc gcctcgccct
    11461 ccacaagtcc ggagaccgcc gtctccagct ggtcgatgcg tttttgtccg gcggcggtgt
    11521 cggtgtagcg ggcaaggtcc cgcacactca ccaccattac ccggcgcggc actgtaatgt
    11581 ccacacccaa tgccagcccg cgttccgcca gcacctgcgg ctgcagcaag ccgccgccca
    11641 acacccagtc ctctagaaaa cggctgcgca cacgctggtc cagccgcttt tcatcctgtt
    11701 cattgccctc gcggataagg atctccgtca tctttttcac gacctggccg tatccgatga
    11761 cctgctcgta cgcgcctgtg atgccgatga cgcccacggt acggcccccg tgtgtgatgg
    11821 gcaggttcag ccccgcccgc acggtaggcg tggcatcctc cggacggacg tacagctccg
    11881 gcaagccctc ctctacaatg cgttttgccc ctgcgtggaa gtggcccacc cgtgtgccgt
    11941 ccgtgctggc aatgatattt ccccgttcgt ccatcatgtt gatgtgctgc cccacgatgc
    12001 cgctgatctc gtccacgata cgctgggcgc tctgtaccga aagctccaca ttcgttctcc
    12061 tttcctcaac gccctgcggc acatggctcc cgccttccgt cccaagccgg ccgcactgaa
    12121 actgtgccgc accgccctgc ccgtattata cactgccgtg agagcatttc ccatggttta
    12181 tatactaaaa taaata
//
LOCUS       DAWBSB010000041        12092 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1253_length_12092_cov_3.548642, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000041 DAWBSB010000000
VERSION     DAWBSB010000041.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 12092)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12092)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12092
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1253_length_12092_cov_3.548642"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2481 a         3364 c         3736 g         2511 t
ORIGIN      
        1 aaataaagag ggcagagcgg aaccgaacgc cgcccgtata caaatccagc atagcgccgc
       61 cgccgccggg cagtcaaggg agaaaagcct gaaattgttt tccggaccct gacggaagga
      121 agaaaaatca aatctttgta taaaatgtat gtgaatcatg cttgattttt gggataaaaa
      181 ttgggcaaaa cacacaaaaa gctattgatt ttggagtttc tgccgtggta tcctcgtaag
      241 cagttggctg ctccgccctc cggcgggcat gcggctgcag gcaggaggcg tacagcgtac
      301 gggtaagccg tccggcctga aaagagtacc ctataccgtg ttttcattac atatggagga
      361 aagcaacatg aaaaagatga aacgcattat cagtgcagcc ctcaccctgg cgctggctgc
      421 cgggatgctt gcaggctgcg ccggctcttc gtccggaaca cctgcggctt cgtccgccgg
      481 cggcgcctcc gccggctcgt cttccgcgga gcgcgaacgc atcacagtcc gcgtattccg
      541 cagcaaggcc tcccatgagg tggccatgga ccagatggac gtattcaagg tgatgggtga
      601 gatgttcaac atcgactttg aatttgacaa tcccccgcag gaaaattaca ccgaacgcct
      661 gaaccttgtg atgatggaaa cggaactgcc cgacgtcatc atggagatcc cggtgacgga
      721 tgtgctgaaa tacggcgaaa cgggcgtgat cctgccgctg aatgattgca ttgaaaacag
      781 catgccgaac ctgaaagcgg aaatggacaa gcgcgacggc gtggagaaag ccctcaccta
      841 ctctgacggc aacatttact atcttcccat gctggatgag acgccctcgg gcaacatccc
      901 gtatatcgtc cgcaccgact ggctggacca gcttggactg gaacgccccg taaccatcga
      961 ggactgggaa aactactggt cgctcgtcaa gaccaccgac ctgaacggca acggaaaaaa
     1021 tgatgagatc cccttcagcg cctatgaaat tgagcgtctg cgcaatttct gcacggcctg
     1081 gggcgtactg gacgatttct acaccgaccc caccgacggc ggcaaggtgc actacggccc
     1141 catcgaggac aaatacaagg aagccctggt ttggatgaac gacatgtgga acaagggcta
     1201 cattgaccag gagatcatca ccacagatta ctcggccttc agcgcgaagc tggcgcagaa
     1261 catcgtcggc tccttcgcgg gcccgctcgg cggcatgctg gccgcccaga acgccacgat
     1321 ggcggaatcc gtgcccggct tccacgtcgc cgccaccgag ccgccgaaag gtgaggccgg
     1381 cgtacagatt cacaccaaca tcgaccttgt cccccgcgcc gttgtgggcg ccaccatcac
     1441 ctccacctgc aaaaatgtag accgcgttgt ggaatggctg gactatatgt acagcgccga
     1501 gggccagatg ctgctgaaca tgggcattga gggcacgcat tacaccatgc aggacggcga
     1561 acccatcttt accgattata tcctgaacaa cccggacggt ctttccccga aacaggcggt
     1621 ggggacgttc accatcgcgc agggaaccat gccctctgtg ctgtgcttca gcgagaccag
     1681 ccagctggac gatgctgccg tgctggaggc caaggagaac tgcatcatcc cgttcctgga
     1741 ggaatccaac aaatatgtga ttcccggcac gctttcgttc tcatctgaga aagacgccga
     1801 gcgccgtgcc gccatggcgg atatcgagac ctatgtggac gagatggtca tgaaattcat
     1861 caccggccgc gaacccatcg aaaattggga tacctatgtg gcaaaagtga aggaaatggg
     1921 cattgagaac gtcattggca tctaccagga agccctggac gcctggaaca gctgaaacag
     1981 aaccgggaag cccgccgcgc gcctgatgtc cgggtgcgcg gcgttctccg gcgaacaccg
     2041 tatgacgggc ggcgggcctg cgagccccgg ccctattttg gaaggaggac acatttatga
     2101 aaaccgccgt ggcctccaga caggcgagac cctcccgcac cgataaaaac ggcagactga
     2161 gccatgcggg caagaagatc tggcgcagca ggtatttgta tctgctgctg ctgcccacgt
     2221 ttgtctggta tatcgttttc atgtatgcgc cgctgtacgg cgctcagatc gcgtttcgtg
     2281 atttcatgcc cgccctcggc atcaccggag ggaaatgggt aggcttcaaa tactttatcc
     2341 agttcttcaa atcggaatat tttgtacgct tgatgaagaa cacgctgggc atcagcgtgt
     2401 attccatcgt ggtgggcttc cccattccgg tcatccttgc actgctgatg aacgaggtgc
     2461 gcagcaagta tttcaaaaaa ggcgtacaga ccatcgtcta tatgccgcat ttcatttccg
     2521 ccgtcgttgt ggtgagcctc atcaatgcaa tgctgtcgcc cagcaacggc ctgttcaacc
     2581 agatcatcca aatgttcggc ggggatcccg tccatttcat ggccgagccg aaatacttta
     2641 agacggtgta tgtgctttcg gatatctggc agacagcggg ctacggctct atcgtctatc
     2701 tggccgcgct caccagcatc gacccctcgc tgtatgaggc ggcgacggtg gacggcgcgt
     2761 ccaaatggaa aaagctgctg cacatcaccc tgccctgcat cctgccaacg atcatgacga
     2821 tgctcatcct gcgtatgggc agcatcttca ccgtgggcta cgagaagatc atgctgatgt
     2881 acaaccccgc cacgtatgag acggcggacg tcatttccac ctatgtatac cggcgcgcgt
     2941 tcgagggcgg cgaatacagc ttctcggcgg cggtgggcct gttcaactcc gtcatcaact
     3001 tcatcgtcat catcgtgttc aataagatca gcaaacgggt aagcgaggtg agcctctggt
     3061 aatggaacgg acaaaacaaa ggacaaaaat caaagagagt acgggggagc gcatttttac
     3121 cggagtgatc tatgcgatcc tgatcctgtt ctgcgctatt atcgttttcc ccctgctgca
     3181 tatcgtcagc ggttctttca gcgaccccat ggccctgctg cgcggcgagg tatcgttctg
     3241 gcccaagggc tttacgtttt ccatgtacga aaaggtattc aaggacgcca gcatctggca
     3301 gggctatgga aataccattg tatataccgt gctgggcacc tgtatcagcg tggtgctcac
     3361 cgcctttgcg gcgtatccgc tttcccgcaa ggatttttac ggccgcaatc tgttcatggg
     3421 tgtgttcgtg ttcacgatgt ttttcaccgg cggcatgatc cccacgtttc tcatcgtaca
     3481 gcggctgcac ctgctcaata cgatgtgggc gctgattctg ccaacggctg tcagcacgta
     3541 caatctcatc atcatgcgca cgttttttga atccaccatt cccttcgagc tggtggaatc
     3601 cgcctcgctg gacggctgca acgaccttgt catctttttc cgcatcgtgc tgccgctttc
     3661 cggccccatt cttgcggtca tggtgctgtt ctacggtgtg gcgcagtgga actcctggtt
     3721 tccggcgctg ttgtacatca gcgaccgcgg cctgtatccc ctgcagatgg tgctgcgtga
     3781 agtactcatc cagagcgaca tcagcaatat ggcgggttcc tcgggtgatg tggagatcat
     3841 cggcgacggc ctgaaatatg ccaccatggt ggtggctacg ctgcccatca tgtgcctgta
     3901 tccgttcctg cagaaatact ttgtaaaggg cgtcatgatc ggcgctgtca agggataagc
     3961 gcgcaggcgt tttttcgaaa agccgggcgc gcaggcgcct gagcggtgcg cccgccggag
     4021 ctccggcaga tgggcgcgcc gcttttttgc cggcactgta aagtaaaaat gaaaaaatag
     4081 gacccgaaag cacaaaaaag ctctatgcaa cgagcagaag ctcgcgctta cgctttttgg
     4141 atagttgaag gccggcacac tgagccggag taaggccatt caaggcggag tgagggcgga
     4201 cgaagttgta gaaaaagatg aacatggaaa tgaggttgtt ggcagaggaa aaagaggaaa
     4261 agccctgttt ggttttgtac catgccttga actgcttgtt aaagcactca atgaggttgt
     4321 tggtgatgtc atcgtggaaa ctttccacgc ggatgtgctg aaccccatgc agcgtcttga
     4381 caggcatttg ataggcgaag taacggtcgc tcacaatggc gcgaggcgag cctaaatcct
     4441 ttaccgcttc cagtatggtg aaagcctgag gactgtccct gtggcggtcc agatggaagc
     4501 caaggacaaa gcgcgtctca ctgtccagaa tgagccagag gtagtatttc ttgccctgta
     4561 tcttaacgac agtttcatcg gcatgccact cgtcagagtt gaaattgagt gctggaatga
     4621 gctgtaaggc catgttttgg aacatcgggg cgaagttggt acaccagtta ctaatggtcg
     4681 tgtgggacac ctggacattc atcacggtac gcagaatcaa tgcgatattg cggaacgagt
     4741 tcttgcccag gtagaacatg ctcaaggccg ttagaatcac atgaacaggg tagcgtatcc
     4801 gcttgaaatc ggtctttcca aagagttggg acatggacgg agcagagata gcggtaggct
     4861 tcggtacaaa catggagtgg ttgcacttct tgtcacagca gcggtagtcg ctgtagtgct
     4921 ccatatcatg gtgcaggaaa gttgccttgc cgcaaatagg acaggaagga tagctcttcg
     4981 ttcgagtata aacacgctcc ggcgtttccg gtgcccactg ataccaacac ttcttgcaac
     5041 gatacttttg atgtccatgc gaatcttttc cgtagcgata aaaatccgtt ttgttgttgc
     5101 acttcgggca gcttatttga tacaattttg ccatgaggac tcgtctcctt tcgggaggta
     5161 cgtgggtttg tggtaaaacc attgtaccag aagggctgac gggtcctcaa ctttcattta
     5221 actttacagt ccgtttttgc catttgggag ggaacagaat gaaaacagaa tatcagaccc
     5281 acgatctttc tctgcccgcg tgggggccgt atacaaagca gtacagcggt atttcccata
     5341 tcccggccgc aaacgacggc gtccggtttg accttgccgt atttcccggg ctgtaccgcc
     5401 gccgtgtgga tgtgcccaac gtacggtttg aaagcggata tcacgtgtgg aaggccgcgc
     5461 cggacctttc ctgctatacg ctgcggcacg agctggaatg gaaagatcgc gtatacgcgg
     5521 acgtttcgtt cgcgccgctg ggcgaaaacg cccgtctgat ccgtgtggaa tgctgcaacg
     5581 ggacagacac gccgcagaat cttgtgctgc attatatggc cagcgcgcat gaccccgtgc
     5641 cctcttaccg caggtttgcg ctgacgggcg cggaagtgcg cctgccggag cacgcggctt
     5701 ttgtgctggc caaagactat gacgacctgc gctttgccgt gccgcgtccc acggacgggc
     5761 tgggatggga cggcatgcgc cgcgccgagg agccggtgga gggctttgcg ggcggatacg
     5821 gcgtggggct tggattcggc tgtgcagccg ggtacgggcc gttcggccag ccgctgccaa
     5881 gcggcgcggg agacgctgta acctatacgt tcacgctgga gcagccgctg cagaggccct
     5941 gtctgtgtgt gcgctatatc aacgcagaca gcgcagcctc gggatatagg ctttcctcgg
     6001 gtgatgtgct ggagctgccg cccgtgccgc agccggaggt agcgcgcatt tgcctgtccc
     6061 ggccgctggc ggcaggcgcg cacacgctga cgctgacggc cgcaggcaca ggcggcgcca
     6121 agctggattg ttttgcgctg tgcgaagcgg cggacagcgg agcgttctgc gtcacgccgc
     6181 gggggagcgg cgcatgtccg gaaatggaat tgtacgaaaa agagggcttc ctcaccctga
     6241 aatatcccca catccgcgag tgctatggcg tattgtggga ctatgaaagc gtgcaggttc
     6301 ggcagatcga aaacagcgag ctggatgttt ttctgcgcga aacggtacac gaccatgttt
     6361 cctccacgct gcacggagat ggaaaaggac atttcaccaa cgtgtttctg cgccccatcc
     6421 cgctggcggc gagacagtcg aaacgggtct acggcgctgt ctgcagcgcg ggcacgccgg
     6481 aggaagcggc tgcgctgtgc cgggagctgc gggcgcggcg cgagagcttt gaggctgtgt
     6541 ggcaggccgc gtcaccggcg ctggagccgg cgcccatgct gcccgcgggc gagccctttg
     6601 ccctcggcgg gcagctgatg cgggcggtgc tgtgcaccaa tgtggtatat cccgtttaca
     6661 cccggggaca gtacatccgt cacaataccc ccggacgctg gtgggacagc ctgtacacct
     6721 gggattcggg cttcatcggc atggggcttg cacagttttc cgcccggcgc gggttcgact
     6781 gtctgaacgc ctacctcacg ccgccgggcg acgacgaggc cgcgttcatc caccacggct
     6841 cgctggtgcc tgcgcagttt tacctgtttg ccgagctgct gaacagaacg cagagccggg
     6901 cactggccga atattgttac ccgcgcctga tgcaatacta tgcatttttt accgggcagg
     6961 cgggaggctc caccacggcg gacctgcaaa gcggcctgct gcgcccgtgg gactacttct
     7021 acaattccgg cggctgggac gattatccgc cccaaaaggc cgtccatgag cagcggctgg
     7081 aaaagcgctg cacgcctgtg gtcacaacag cgcacgcggt acggtgcgcc cgcatcctgg
     7141 cctatactgc gcacctgctc gcccacgggg aggacgcaga ccggctggaa aaggatgcgg
     7201 agcgtttcac ccgggcgctt caggagcacg cgtgggacga gggcgccgga tacttcgggt
     7261 atgtgctgca cgatgaacag ggaagggctg ccggcttttt gcgggacgct tccggcgtga
     7321 accataacat ggggctgggc ggcgcatcac ctctgtttgc cggcgcgtgc actgtggagc
     7381 aggccgaagg cctgtgggaa aagctggcga gcgaagagca tttgtggagc gcctgcggcc
     7441 tttccacagt ggaccggtcc gccccctatt accgcaggga cggatactgg aacggcgcgg
     7501 tgtggatgcc ataccagtgg atgttcttca aagcggcgct ggatgcgggc aaaaccggct
     7561 tcgcgttccg tatcgcggat acggccctgc ggctgtggca ggatgaatgc gcggcgtcct
     7621 accattgctt cgagcatttt atgatcgaga gccgccgggg cgcgggctgg caccagtttg
     7681 gcggcctttc cgcgcccgta gcacagtggt ttgcggccta ctacgtaccc ggaacgctta
     7741 caaccggctt cgacgtcttt gtacagtgcg cgtgttggct gcccggaaac gcggggctgg
     7801 aggcggagct ttattgtacg cggcccgggc gtactgcagt tctggcggcg cttgcaccgg
     7861 gtaaaatgcg catttcctgc cgtttgggcg tggcggcgga acagcgccac gcaggcctgt
     7921 gggagatcac gctggacgtg gagcagccgg gcccggtgag actcaacctg ctgccggagg
     7981 ggagctgcgc cgaatgatac gcctggaatg gaaggactgc cgccttacgg cggagggcac
     8041 gcaggtcgtg ctggaaaatg ccgcgctgcg cacatgctgg aggcttgagc cggagggttt
     8101 ggcgctggcc tgcgtggaaa accgccttac gggcttttgt tggcagagtg cggacggtgc
     8161 ggcactggct ccggcctgca tggccccgcc ggccggggaa ccggaatggg aggcccggcc
     8221 gcaggcggca ggcctgtgtg ctgagcactg gtccgtgcgc tgcacctgga ggcagggcgc
     8281 gcgcagtgtg agccggtgtt ttttgctgta tccgcaaacg ccgttcctca cgatatgggc
     8341 cgaatgggag gctcctccgg aaggggtgtt tgccgctgtt cggcagacag ttcccggcgc
     8401 gacaggtgta gagcgggaca gcgcgcgtgc ggcggcgcac agcgtgcccg gggatgttat
     8461 ggctgccgtg ccgctgccac gcggtcatct gcggtggcgg tgcatacgcc ttgcggacca
     8521 aacggatgtg catgacactc ttgtgtggga gcagggcgcg ccggtctatc ccgcagagcg
     8581 tgtagaggcg tccggaagtt tctttaccgt ggaagacaca cggacgggag aaacactgct
     8641 tgcggcccgc catgcgccca taacgccggc gcccggcccg cagtttcttc tggcggagaa
     8701 ctgcttgtgt gtactgcaaa acggttgtgc aggcgcgccg cccgccggaa cggtgggatg
     8761 tacgtgtgta ttgggcaccg cgcctgcaga acggttgtgg gagcagtacc gtgcgtttta
     8821 ccggctgggc tggcaggcac cgtggcagcg gcatgtgctg ctgctttcca acacctgggg
     8881 agaccggaac agggacgcgc gggtgtgcga gacgttcgtg ctgcaggaga tcgaccgcgc
     8941 cgcagcgctt ggcctggaca cggtgcagat cgacgacggc tggcagaagg gcacgacggt
     9001 gaattccgcg cgtccgttgg ggggcgtgtg ggagggctat tacgcggcgg atgcggactt
     9061 ctggacgccg catccggaac ggtttccccg cgggctgtac ccagtggcgg agcatgccgc
     9121 cgcgcgtggc gtagcgctgg gcttgtggtt ctctccggat tcctccggag aatttgcaaa
     9181 ctggcggcgg gacgcggaaa cgttgctgcg gctgtggagg acctatggag tggcggtatt
     9241 caagctggac ggcgtcaagc tgcgcacgcc ggcggcgcgc gcgaaatacc tttccctgct
     9301 ggaaatggtt acagcacaaa gtgggaggcg cgtgatgctt cagcaggaca tcaccgccga
     9361 gcagcgtatg ggatatctgg ctgcgcggga atacggcacg ctgtttgtgg aaaatcgcta
     9421 cacagatttc ggcaattatt atccccatcg tacgctgcgc aatctgtgga tgcttgcccg
     9481 gtatgtcccg gcccagcgga tgctgttcga gctgctgaac ccggcgcgca acacagagcg
     9541 gtatcgggcc gacccccttg cgcccggacg gtatacggca gactatctgt ttgcgtccgt
     9601 tatggccgcg cagccgctgc tctggatgga gctctccggg ctggggcggc aggacgcggc
     9661 gcgcctgcag cagatcatcg gtgtgtaccg tctgcaccgg gaggcgatgt gggcgtgcga
     9721 tgtgcgcccg gtcgggcagg aaccggatgg ccgctcgttc acaggctttg catttacaag
     9781 tccctgtggg cagaagggat atctcctgct gttccgtgaa aatgcgccgg aaagcgcctt
     9841 cacctttacg cggatgccgc agaaggcccg gctgcgcctg ctgtgtgcaa acggcccggt
     9901 tgggcaggga tacacgcccg caggcgacct gtgcctgcgt tttgcagccc cgcgcaccta
     9961 tgcgttttat cagtggcaga cataaagcgc cggccaaaga ggcgcacaaa aaacggcagg
    10021 gcatgcacgc cctgccgttt ttggcagaaa gagaggaacc ggtcagcctg cgttgtcccc
    10081 gcggcgcacc gccgagggcg aaatgccgaa cctgcggcgg aaagccttat aaaacgtgtt
    10141 ttgcgtggaa aaaccgcatt gtacggaaat ttcggttata cccagctcag agccgcgcag
    10201 aagctccatt gcacgggaga gccgcaattc ctccacatac tcggtaaagc tcttgcctgt
    10261 ctggtccttc aaaaactgtg tgacgtattt ttttgagata tggaactgtt cggcagccat
    10321 ggcgatgttg aggtccggcc gggtgtactg ttcgttcagc caggcgagaa cgcgccgctg
    10381 aagagcttcg ttgtggctgc gcttgaggga atcgacatgg ctgcatatct caaagcagca
    10441 gtcgcgcagg ttctgcacct gccgcctggc ggactgccgg ttatcataag agcaaagata
    10501 ggcgatgtct tcgcactcca ccttttgcgc cgcggcgatc agcacaccgc gcacgctgaa
    10561 gaatgcctgc tgaaagttct gcggcgaaag atcgtccaca tggaacagtt cgccgatgag
    10621 agactgcgct tcctgtgcgc ggccggataa aaggtattca ttcagccgtt ccagactggt
    10681 gatatcggtt gttgttacac ggacaaggct ttcgccgctc agatagcata ccttttgtgt
    10741 gcggtcggaa taagcggcca tgttgcgcgc ctgccaatat acagaggaaa gctgttccag
    10801 ccgcgtaaaa cgtccgctga gaatcagcgg ttccgtcatt tccaaaaggc cgtgcagagc
    10861 ctcggtctgc cggcacatct gtacttgctc ggcttcggta cctttgtcca gcgtcagaac
    10921 aacgagtacg ctgccctcgg tggtgttgta aacgaactgc gtgcgcccgt agctctggcg
    10981 cacctgctcc gcgatcatca tcaagcgcag ctcctcgccc acgtgcgtct ctgtctgccg
    11041 ctcctgcgcg gggatcaaaa gaagatagtt gtagtccgtc agatgcatct gttcgcacat
    11101 ttttttctgt tcttcggccg tgcccgctgc gccggtcttt aaaaatgctt ctacgcggct
    11161 atggtccagc tcgccgcggg cgcgttccag ttcagccgaa agctggctgt ggttgcgcag
    11221 cttctggacg agcagatcgt tgaacagacg ggtgttcgcc gtttcggcat catccagcgt
    11281 gtccagtatg ggcttgagct ggcgcagctc ccacagcgtc attgcggaac tgagcaaaaa
    11341 gcagcccagc agccccgcac cgatgtaaaa ccatatggcg cggttgacgg cgcgcaggtt
    11401 ttcggcgtaa acggaatagg gcaggccaat cacggcgctg gcgccaaggc tggggatgct
    11461 acgggaaaca aacagatact tctgcccatc ataccattcg attcccgttt catttccgga
    11521 gaaggagagg gcggccccgt tgcagcggta ctggctcagc aacgtgccgt cgtcccgcac
    11581 gagataaaac aagccgtcat tgcgccattt ttccgtgggc agaaacaggt tggacagagt
    11641 gcgttcccga atcagtatat tgcaggcacc gcggaattgt ccgccggagt tgatgaacgg
    11701 ctgcgtgatc gtcagatagg aatccggata attcgtgcgg ttcagcacaa cgctctgtgc
    11761 gggcaggtaa agcgtctcac gcgcgtcctg ccatgcggcc caggtgtcct gggggacatc
    11821 gcggtactcc agcgcgcccg gataaaagct ctcatatccg gaaaaaacgc gataagggtc
    11881 gatcacaaaa tggttgcgcg caaaggtgac gtagctgtaa gcggtgtaag acatgctgta
    11941 agtaagatcc tgaagcagtg tggaagcgtt gaaagacgtc atgtagtcgc ccatcagcga
    12001 atcgtcatcg ctcacggcca gcagtttgta ggaatcgtcg ttgaacagct tgttcgtggt
    12061 aaaccgcagg tcgctgagat atccttcaaa gg
//
LOCUS       DAWBSB010000042        12008 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1261_length_12008_cov_3.788170, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000042 DAWBSB010000000
VERSION     DAWBSB010000042.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 12008)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12008)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12008
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1261_length_12008_cov_3.788170"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2567 a         3531 c         3670 g         2240 t
ORIGIN      
        1 gccctctgtt gaggtcaacg ttctgcaggg cgagcgcgag ttcgcgcggg acaacaaatc
       61 tttaggtact ttccatctgg acggtatcgc tccggcgccc cgtggcgtgc cgcagatcga
      121 agtcaccttt gacattgacg ccaacggtat cgtgaatgtt tccgccaagg atctgggcac
      181 cggcaaggag cagcatatca ccatcacctc ctccaccaac atgagcaagg aggatgtgga
      241 aaaggccgtg aaggaagccg agcagtatgc ggccgaggat aagaagaagc gcgaggaagt
      301 ggatatccgc aacggtgccg accagatggt gttccagacc gaaaaggcca tgggagaatt
      361 cggcgacaag gtgtctgaaa gcgagaaaac cgaggtgaac gcgaagctgg aggcgctgaa
      421 ggaagcgctg aagggcagcg atatcgaggc catcaaggcg aagcaggagg acctgcagaa
      481 ggcgttctac gccatcagcg agaaggtcta taaggacgcg gcggcggccg ggcaggccgc
      541 gggcgcgcag ggcgccggcc ccgacatggg cggccagccc gcgggcggcg gcaacggcgg
      601 cgacgacaac gtcgtggacg ccgagtacaa ggaagtgtaa gcacccccgg cgggtgcgcg
      661 gaagagggac agccgtgcaa agcggctgtc ccaaaccgct tttgcggggg cgcggcttta
      721 caatgcgccg cgcctctgtt acaatatggg agctgaacgg gaggccccgc gttcagccgc
      781 gcacggcgga ccaagacgga ccgccgcgtg cccacttctg aagaaatcaa ggtgagaaag
      841 tacaatggca gataaacgcg attattacga ggtactcggc gtgcccaaaa cggcctcgga
      901 cgacgagatc aagagcgcct accgcaaact cgcgaaaaaa tatcacccgg acctgaaccc
      961 cggcgacaag gccgctgagg aaaagttcaa agaggtgggc gaggcatacg agatcctcag
     1021 cgataaagat aaacgcgccc ggtatgacca atttggccat gcaggcgtag accccaacta
     1081 cggcgcgggc gcgggcggcg ggttcggcgg tggctttggc ggcggcgtgg acttgggcga
     1141 cctgttcggc gacctgttcg gcggcggctt cggcggcttt ggcggcgggc gccgcgccaa
     1201 cccaaatgcg ccgcgcaagg gcggcgatat tcgcgtcagc ctggtgctca gctttatgga
     1261 ggccgtccac ggctgcacca aaaccgtcag cgtcaaccgg caggacacct gccctgaatg
     1321 cggcggcacg ggcgcggcca agggaaccag ccccgagaca tgcccggatt gccacggctc
     1381 cggctatgtg acggtgcagc agcgcacgcc ttttggcgtg atgcagagct cgcagccctg
     1441 ctcacgctgc ggcggcaagg ggaagatcat caaatccccg tgctccaagt gccacggcag
     1501 cggcaagacc tccagcacaa agcgtgttga ggtaaaggtg cccgcgggta tcgacgacga
     1561 tcaaagcatg cgcctgacag gtctgggcga cgcgggcctc aacggcgggc ccagcggcga
     1621 cctgatcgtg atcgtgacgg tgcgcccgga cgccatgttc gagcgcgacc gcttcgacgt
     1681 acatgtcaca gtgccggtca cgtacagcca ggccgttctg ggcgcggagg tggtggtgcc
     1741 caccatcgac ggcaaggtac agtacacggt gccggaaggg acacagagcg gcaccacgtt
     1801 ccgcctgcgg ggcaagggca tccagtatgt caacggccgc ggtcggggcg accaatatgt
     1861 taaggtcgtg gtggagatcc ccaagaagct gacaaagacc cagcgcgatg cgctgaagaa
     1921 atttgaggat acgctcaagg acgataacta cgagcagcgc aagggctttt tcaaaagcct
     1981 gcgggataaa tttgaaaacg gctgagtccg gtacgggcgg cgcgggatat tcccgtgccg
     2041 cctttttgtg tgaaatttac ggcggcatgc aaactctgcg cggttcgtga tataataacc
     2101 ttaagcccgc cgcttgcgcg gccgcgcgtg tgcgcagcac acgctgatgc gccggcgcgc
     2161 atgggcttga gaacattgaa acacgcaaaa ccgcagcagg cgcggttttg ttgtggccgc
     2221 ttcgcgccgt cgtgttataa tagccttaag cccgccgctt gcgcggccgc gcgtgtgcgc
     2281 agcacaaaaa aggcagggag ctgaaataaa tgagccttaa ggattaccgt tttgacggtg
     2341 aagaaaagct ggaactgcgc ggtatgaata ccggcgcgaa aaaagacggt gtggacaagg
     2401 aggatatcct ccggcgcact gaaaaaaatc tggggcgcat cgaggcgctg caggacaagc
     2461 tgtatgcgga tggacgggag tccgtgctca tcattctgca ggctatggat gccgcaggca
     2521 aggacagcac tgtcaagcat gtgatgggct gcgtgaatcc ccagggaata gacgtgcaca
     2581 gctttaaaca gccaaccagc gaggagttgt ctcacgatta tctttggcgt gccatgaagg
     2641 cgctgccggc ccggggcaag atggcgctgt tcaaccgctc gtattacgag gatgtgctgg
     2701 tggtaaaggt gcatgcgctg caaaaaaact acaaaatgcc cgcacgcgtg ctggaggacc
     2761 cggacttttt caaaaaacgc taccggcaga tccgccattt tgaggaatac ctgtatgaaa
     2821 acggctgccg cgtggtgaag atattcctga acctttccag agaaaaacag aaagagcgtt
     2881 ttctggagcg catccaggtg gaggcgaaga actggaaatt ttcccaggcg gatatccggg
     2941 aacgggccta ctggaacgat tatatggacg cctacgaggc ggccgtaaac gagacggcta
     3001 cccgccacag tccgtggtat gtgatcccgg ctgaccagaa atggtacacg cggtatctgg
     3061 tctccgaggc ggtgttgggc gtgctggagg atatcgaccc ccactatcct aagcttccgc
     3121 ccgagcatgc ggcagagctg caggaatgcc gtgaggcgct ggaaaacgag tgacgcggcc
     3181 ttccgcacag aaaaagaaca aaaaagggcg tttccccggc agcatcgctg cggaggaaac
     3241 gcccttcggc atcattggga ggggacggga gcaaagaccg gcccggccgg tcagacggcg
     3301 gattcgagca cacgctcttc catcagctcg ctgccgcgcc atgcgcggaa caccacgcgc
     3361 aggcggcacc cctctgcggg cgtgcccggc agtctggcgt ccagccgcac gccgccccgg
     3421 aaggatgcct cgacctccca gccgccgtct gcacgcggac ggcgcagctc ggcgacgacg
     3481 gtgcccgtac agccgcgcgc gggacgcatg cctgccctga cgtgcagtgc atctctgccg
     3541 ggagcgggtg cttcgcggcg cgcggcgcag tccgccgctt cgatccattc gcccgaaggc
     3601 agctgcgccc acagcgcgcg cgcacggggt gcggccgccg ccgcgcataa aagcagcacg
     3661 gcaaggccca acaggaaata caggcgtcgg gagcgtgtca tggggcggcc tcctttcaaa
     3721 aacagcggga aagagcggtt tacagctttg tttatagcat acagttgttt ctatcttgta
     3781 aacgaatgca cgcaaaatcg cggtttcgaa atgaatgacc ggagttgaca gagccttcga
     3841 atcctcctac aatcaaaaca acggcgcggt acccgtgcgc cggaatgaaa aaaggcacgc
     3901 gtgtgcaagg aggaaatatg cagcttcgat tggagcccgt gacgcaggaa aaccggctgg
     3961 ctgtgctggg cctgcgggca ggaacggggc aggagggctt tgtggagaca gtgtccgcat
     4021 gcctggacga ggccgcacag cgcgcggact ggagacccgt ggccatttac gacggcacgg
     4081 cggtggtcgg tttcgccatg tacggttatt tttgggaata cccgcctgcg ggccgcgtgt
     4141 ggctggacag gctgctcatc gacgccgcca ggcagggccg aggctacggc agagccgccg
     4201 tggcatctct gctggcccgc ttggcggagg aatacggccc gcgggatatt tatctgagcg
     4261 tagtggacgg caacaccgct gctacgcgcc tgtatgaggc gtttgggttc gtcttcaccg
     4321 aagagaggga tgtccacggc gagcatgtga tgcgccgccc ggctgcacct gtacgcgggt
     4381 cgtaaagggc aagctgtaaa acaaaacagg gccgccccat tccgcgctgc gggaacgggg
     4441 cggccccttt tccgtttggg gaatggcaaa ggccttgctt ttcaggccgc ctcatcctct
     4501 ttcgtccggg cagcgtccag cggtacggcg gtttcctccg gagcaatggt cctgatgccg
     4561 aagataaagt acaggatatg gcatacccac accaccgcca atacggcacg tcccgcaggc
     4621 acacgtccca tcatggcgaa gccgaaggcc atcgtcagtg tgacaacgcc catgatgcgc
     4681 agcttggctg cacgggtcat gcctcgtccg cgcacaaagc tttccagatt attcttgtac
     4741 agcctggtac tcaggaacca atcgttcagg cgccgggagc tgcgggcaaa gcaaaaagcc
     4801 gccagcatca ggaaaggaaa cgcgggcagc agcgggacgg cggcgcccac cgcgcccagg
     4861 cccagaccga tacagccaag gatcacgtaa aaaagcttca ttctgcagcc tccaattttt
     4921 tcttttcgcg ttttgtctca atcacatgcc gtgctgccgc aacaggatgg tgcagcaaca
     4981 tgcggggccc ggcccaacgc atcacagcac gaattttttc cctcatatca ggacggtagc
     5041 agtgtacact gcagttggag cagaaggttt ttgtctccat gaacggacat cggtcgctgc
     5101 gctgccccgc atattcggcc aacgcggcgc attcggggca caggcgtttt tgcccgccgt
     5161 gctttttgcg gcagtacagc gcgaccattt cctgtaccat acgcttttcc cgctgccgct
     5221 tggcctcaac gctctgcagg cggaccatca gctcacccgc ccttcctgcg cggcgtatac
     5281 ctcgtcggca acggccattg tggatgcccg gtgtgagacc agcaccacgg tgcggccgcc
     5341 gcgttcgctt tgcagcgacc ggagaataac gccctcgttc aggctatcca gattgctggt
     5401 gggctcgtcc agcaacagaa acggcgcgtc gtgcaggaac gcccgggcaa gccccagccg
     5461 ctgccgctcc ccgccggaga gcgtatctcc cagctccccc acaggcgtgt catacccctt
     5521 gggcagggac aggataaatt cgtgtaccga agcctttttg caggcagcct ccagctcggc
     5581 ctgcgtcgca tcccgcttcc caatgcgcag gttgcttgcg atgctgtcgc ggaagaggtg
     5641 cgtttcctgc gtcacaaagc cttccatacg ccgcagatct ccggtgttca cctcccgaac
     5701 gtcccggccg gagacgcgca ccgcgccgcc ggccgcgtcc cagaagcgca tcagcaggcg
     5761 cagcagcgtg gatttgccgc tgccgctgcg ccccgtaatg cccaccacgc ggccctgcgg
     5821 gatgcgtagg ctcacacggg agaggactgg ctcttcgccg taagagaagc tcacatcctc
     5881 acaggctgcg ccggaaaagg cggtggtctg cctgccggcg atctcatgca ccagcggttc
     5941 ctcatccagg atatccagca cacggttgcc tgcggccagc gtattttgca gcgtactgcc
     6001 cagatttgcc agcgctacca caggcccaaa ggaactcatc agtgcggtca gagggatgag
     6061 cacgccgtcg aatcccaccg cgccggcaaa atacagcccc gcggctgcgg ccagcattcc
     6121 aaggctgaac gtcagaatga cggtgttcgt cagagccatg ctccatgccg cgcgcccctt
     6181 catgcgttcc tcggtgtgca gcaagccttc tgtgcgctcc tccaaccctg ccaggcggtt
     6241 ttcgccctga tggtattgca gcacttcgcg cagaccgcgc aggctgtcca gtacatagcc
     6301 gctcaacgct ccggcctgtg cacggaactg tgcgcccgta ttgccgccca gccgtgcgat
     6361 gagcaccggc agcgccgcac ccaccgtcag gtatgccgcc gcggccagca gacccagcag
     6421 cggatggtaa gcaccgatga acacactcat gccgacagac atcacaagcg cgatggccgc
     6481 gggagagatg gtatgggcgt aaaacacctc cagaagctcg atatccgcag tgatgacggc
     6541 aatcagatcc cccttgtccc ggccctccag tttggcgggg cacaagcggc gcagggcagt
     6601 gaacacctta tcgcggatga gtgccagcag cttgaaggcg atgaaatggt tgcaggcctg
     6661 ttcgccgtag cgcagaacgc cgcgcagcag ggcgaaagcc gccgcgcagg cgaacaggcc
     6721 gcccagcgcc aggggagcgc ccagccccag aacctccagt atggcccagc cgcccagaat
     6781 ggtgagaaat atggcgcaca caaagcccgc cacgcccagc aggacagcca gcgccataaa
     6841 cggagccaga gggcgcacca gcacgatgag gcggcgcatc acagaaaagc cgcttcgctg
     6901 ctgtttcatg cgctcacctc ctctgtccca aagtgttcca gctcctgctg tctgtcccaa
     6961 agcgttttgt aaaggcctcc ctgggccagc agtgcggcgt gtggaccgtg tcccgccacc
     7021 gtgccggcct ccagcacata gatgctgtcc gcgtccgtca cgttggcaag gcggtgcgtg
     7081 atgacgatga ccgtccggcg cctggccagc gcgcggatct cagccatgat gtcgttttcg
     7141 ctctccacgt caatgttgga tgtggcttcg tcgaacacat agaccgggct gtcgcgcagc
     7201 agcgcccggg caagcgcaag gcgttggcgc tgcccgccgg agaggttcgc gccctgagcg
     7261 tccagcgggg tgtccagccc tcgctcaccg cgcaggaaac ccgccaggcg cacgcgttcc
     7321 agcacgtccc acagagacgc gtcgtctgcg tccggccggg ccatgcgaag attttcggcc
     7381 acggtgcctc ggaacagata gctgctgaag cctacatagg tcacggtgcg cagcaggctt
     7441 tcggcggaaa gagcggaaat ctcgccgccg ctcagcgccg cgctgcccgt gtagccccgg
     7501 cagcgccctg tcagcacgga ggccacagtg cttttgccgc atccgctttc acccacgatg
     7561 gccgtgaacg cgccttgggg aaagtccatg gaaacgccgt gcagcacttc gcgcccttcc
     7621 tcgtaggaga aacgcaggtc cctgcaggcc agcgtgccgc ccgctgccgt accgctgcca
     7681 ggggcgtcct cgggcaggtc cagcagggta aagatcttat cgctggcagc catgccgttc
     7741 atcgcgatgt ggaaaaacga gccgagctgg cgcatgggca ggaaaaaatc tgccgacaga
     7801 aaaatgacgg cgaagcagcc ccagaacgga atgcgccccg cgcggaattc aagaacggcc
     7861 agcaccatgc ccagcgccgc cccgccgtat gccacgagat ccatgatggt gatggaattg
     7921 agctgcatcg tcagcacttt catggtgatc ctgcggaatt tttccgcctc ccggttcatc
     7981 tcctcctgct tatagccgtc ggcctgatag atcttcagtg tggtcagccc ctgcaggttt
     8041 tccagaaaag tgtctcccaa tgccgtatac tggccccagt agcggcccaa cagctttttg
     8101 gcaaaggtct gcaccgccgc gatggcggca gggatgagcg gcacacacac cagaagcgcc
     8161 agcgcggccc gcaggctgac gggcgctaac agcgcgaaca atgtcgccgg agcaagcatg
     8221 ctgtaaaaaa actgtggaag gtatgcgccg aaataggttt ccagttgttc cacgccctcg
     8281 gcagccagct gcacggcctc ggaggtcgcc acgttctggc tgtatgcggg gccaaggcgc
     8341 agaagctttt cataaatact gcggcgcagc gtgcgcttca ccgagcgcgc ggccagaaag
     8401 ctcatccggc ccgcacctgc ggtgcatatg ccccgcacaa ggacagctgc cagccccgcg
     8461 gccagcggca gtgcaagcgt gcggggggcg gcggcgccct ccagcagctg ctgcagaaac
     8521 cacgcgcagg agaagatcat ccctgcattg ctcagcaagg aaagccactg aagcgccacg
     8581 tttccggcga tgtacggttt gctgtccggc acggtgccga tcagccgttt gttgatcatc
     8641 atggacaaac tcctttttga aggttaggaa aagctaaccc gtgctgaaaa caacagttag
     8701 tttttgctaa ctaactgttt ttatagctta tctgtccggg cgggatttgt caatccctct
     8761 atggcatcgg gtgaaaaggg aagaccttcc ataaaaacgc tgcagcggca gtgcaaaata
     8821 tcaattgtat gcctttccgg aatatgctat actttgatac agtaacaggg tgatgcagaa
     8881 aagaaacata aatgaagagg aggaagtgtt gtgatcgacc ggaaatgcaa agtgacgctg
     8941 ggcggcctgc cgcagaagat ccatgtcaaa ggcgatgaaa cgaagccggt gctgctgttt
     9001 ttgcatggcg gcccgggtgt gtgcaaccgc cacacagtca tgacggtgca cgcagacctg
     9061 caaaaggagt ttcttgttgt cgcatgggac cagcggggca gcggcggttc ttatcgcggg
     9121 gcggccgtgg agacgctgac gatcgaccgc cttgtggacg atgcccacga gcttgtgctt
     9181 tggctgtgtc gggaatacgg caggaaaaag gtgtacctgc tgggcggcag ctggggctcg
     9241 gagctgggca catggctggc gtaccgctat ccggagcaca ttgcggcctt tgtgggcttc
     9301 gggcaggtcg tcaatggggc gaaaaacgag gaactgagct atcaatttgc gctggatgag
     9361 gcccaaaagg ccggggacga aaaatccatc gccgtcttaa agcgggtggg gcccccggtc
     9421 atgggtgttt ataagggcgg cttcgacggg atgatggccc agcgccgggt gatgatgaaa
     9481 tacggcggct attcccaaag caaaaagaaa cgcagctatt tccgttccat ggtgatcccc
     9541 atgctgtgct ccggcgaata cagcattccg gacctgatcg gcgtggccaa agggtaccgc
     9601 tatgtgctta cagccatgtg gcccgccgtg ggagcaacgg actttcccag tacctgtaca
     9661 aaattccagg tgccttattt tgtctttgac ggcgtgctgg accagaacac ccctgcggcg
     9721 ctggtgcagg attatttcga tttgatccag gcaccgaaaa aggagctgat ctggttcgag
     9781 cagtcgggcc acaacccgat gaccgacgag ccggagcgct tcaaaagcct gctgcgcgac
     9841 cgtttgcagg cccttgaaaa ggaggaaccc accgtatgaa gcaggatgtt cggacaaatg
     9901 tgatcccgcc tttgaaattt ggggaaaagt tcggcttttt gaccttttcc actgccagca
     9961 acgtggtgta ccagttcaag agcatttatt atctgttttt cctcaccaat gtactgaaga
    10021 tcgacgtgct cgtcgcgggc atgatcctga ccatcggaac cgtctgggac gcggtcaacg
    10081 acccgctgat cggcttctgg tccgtgaacc acaaattcaa aaacggcgag cgctgccgcc
    10141 ccttcgcgct gtggttcgcg gtgccctggg cggtttcggt ggtactgctg ttctgcgatt
    10201 tcaaaacctc gcagaccgct gccgtcattc tggcgctggt gatctatttt atttttgaat
    10261 ccttcaatac ctttgttgcc atcccttaca acagcatggg cggcctggcg accaaccgcg
    10321 acgcggaccg gcgttccatc aacgtgtacc gcaatttggg cggctgcatc ggctcgggca
    10381 tcggcgctgt ggcctgcctg ccgctgctca agctgttcgg cgcgctggac aacggcggca
    10441 acctcatcga cggcagcagc tcccgcgggt tcctgctcac cgccatggtc atgggcgctg
    10501 tctgcatcgt cggcagcctc acacactatt tcaccacaaa ggagcgcgtc gtacagatct
    10561 cggacgacga ggaccacgta agcgcccgca gcgcggccaa aatgctgttc cgctgcaaat
    10621 cgtgggtgct caatatgctg tacatcatct gctatggcgt aaacaacgtg ctcatcatga
    10681 gctccatcac ctattatgcg acgtatgtga tgggcagcac cggcgcggcc accatcatcc
    10741 aggccgtgta cctggtggtt tccatcatca cgacggtgct gaccggcccg ctggaccggc
    10801 tgctcggccg caagcggacg atgatctttg cggccgctgt actgatcctg ggcaaagtat
    10861 ggttcgtcat cgaccccttt gcggtggggg caatctatct gaacgcggtg acggtgggca
    10921 tcggcctttc catcaccttc gtcatgttca ataccaatcg caacaatatc gcggacctga
    10981 tcgaatggca gagcggacgc cgcatcgaca gccttgtttc aacaggcgat aacctggcgg
    11041 caaagctggc ccaggcgggc gcgacgcagc ttctgacgct ttctctgcac atggcgggct
    11101 tcaacgaggc gctgccggcc cagccggacg cgaccatcgc cacgatcaat gcgctgctgg
    11161 gctgggtgcc ggcggcggtg acctttgtga tgctggctgt tctgtgcgcg atgaacatcg
    11221 aaaaggacat ggacagcatg aacgcggaaa aagccgctcg gggaatctct gtctcagact
    11281 gacggatgtg caaagtcagg cttccggtta agctgagatg gtcaatggat aataaacagc
    11341 aaaaattaac aaaagccttg ttcaaatata aattggacag ggcttttgtg atggacgttt
    11401 gaaagaaggg gggaagacga ttctcatttt cgcctcatat aataaaagaa cagtagacac
    11461 gagagactgt gtctactgtt ttttaccgct gtttccattc tgttatggcg ccgcctgttt
    11521 gtagaaatac gccgcgtttg tgtgctgcac tccttttgcc tgagtcttgc cgggtctttc
    11581 aatggaaccg gaaacgtgct ttcgcttcgg cacctgtaaa aatacggtac atctgcagag
    11641 aacctttttg tacagccttt cactcctccg gctgtctcaa caatttctgt tcttcatccg
    11701 cttcaacaat tcccttgtaa aaatcgttgt atctggcgga agcgtcgtca tcttttgctt
    11761 ccatgcagac agatatgctg tttggatgaa gatctgggtg tcgagcgcct gctgtacagg
    11821 gcacaaaacc ggttcgtttt ttgtgattgc agaaatgaag ttatttatga taccagcctg
    11881 caagatactg ttctcctcat ttttaaaagt gaacagctgc tctgtgtaag gaatgcgccc
    11941 gtaaggctcg ctgctttgta aagaataatc cttttcgtct tgctgcagtg aacgaagagt
    12001 taaaatgc
//
LOCUS       DAWBSB010000043        11935 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1266_length_11935_cov_3.526852, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000043 DAWBSB010000000
VERSION     DAWBSB010000043.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 11935)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11935)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11935
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1266_length_11935_cov_3.526852"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2202 a         3480 c         3731 g         2522 t
ORIGIN      
        1 cgggtgagct cctgatacac gatacggcct gcgccgatgt tggtgtggcc cacctcgctg
       61 ttgcccatct ggccgtctgg taaaccgacg ttcataccgg aagcgccgat ggtggtgtaa
      121 ggatactgcg cgaacagctt atccaggttg ggcgtcctgg cggcaaggat ggcgttgccg
      181 taggtttggt cgggcaccca gccgaagccg tccagaatgc agagaagcag aggttttttc
      241 atagatggtt cctttctgaa aaaagaaatt gaatggggat gcgccgcgcg ggcagcgctg
      301 ttttgcaaaa agagcagcgg aaaattattt gcctgccgcg gcaacaatgg cggcaaagtc
      361 cgcaggcttc agggaggcgc ctccgatgag gccgccgtcc acatcaggct tgctcagcag
      421 ctcgtcggcg ttggcggcgt tcatgctgcc gccgtactgg atggtcgtgg actcggccac
      481 tgcctcgccg tacagttcac ccaccacctt gcggatggcc gcgcagactt cctgcgcctg
      541 gtccgccgtg gcggtgcgcc ccgtgccgat ggcccacacc ggctcgtaag cgatgatgac
      601 gcgggccatt tcttcagcgg aaacgccgcc cagcgcgatc ttcgtctgca tgcgcacaag
      661 ctcctcggtc acgccctgct cacgctgggc caggctctcg cccacacaca cgatcaccgt
      721 caggcccgcg gccaaagcgg ctttggtacg cttgttgacg gtctcgtccg tttcggcaaa
      781 gtactggcgg cgctcagagt gaccggtgat gacatatttc acgcccagat ccaccagcat
      841 atccgcggat atctcgcccg tgaacgcgcc ggatttttca aaatgcacgt tttctgcgcc
      901 cacaccgatg ttggtgccgg cgcatttttc cacggccgtg cagaggctgg tgaacggcgt
      961 gcagatgaca acgtcgcagc ccgctccctg tacggcggga acgagcgcgt cgatgagaga
     1021 cgcggcctcg gtggcggtct tattcatttt ccagttgccg gcaatgacgg ctttgcgttt
     1081 tgctttgttc atcgtatatt ctccgttctg aaagatattg cccggcgtcc cgccggagaa
     1141 aaaccgcagg ggcaggcggg attcccgccg cccctgcaaa agagtcttat gcgcccgcga
     1201 tgcaggcgat gccgggcagc tctttgccct ccaggtattc caggctggcg ccgccgcccg
     1261 tggagatgtg cgtcatcctg tcggcatagc ccagctgcat cacggcagcc gcgctgtcac
     1321 cgccgccgat gaccgtggtg gcgtctgttt cagccagggc tttcgctacc gccttggtgc
     1381 cggcggccag aaccgggttc tcgaacacgc ccatggggcc gttccacaca acggtcttgg
     1441 cgctcttcac cgcgccggca aacagctcca tcgttttggg gccgatgtca aggcccatca
     1501 tgtcggccgg gatcttgtcc gaatccacca cttcgacggc aatctccgcg tcgatggggt
     1561 cggggaaggc ggaagccacg gtggtgtcta ccggcagcag cagggaaacg cccttttcct
     1621 cggccttctt cagcatatcc ttgcagtaat ccaccttctc caggtccaca agagatttgc
     1681 cgacttcgca gcccttggcg gccaggaacg tataagccat gccgccgccg atgatcagcg
     1741 tgtccacctt gttcagcagg ttttcaatga cgttcagctt atcggcgact ttcgcgccgc
     1801 ccaggatggc cacgaacggg cgcacagggt cgttgacggc gttgcccagg taacggatct
     1861 ctttctccat caggtagccc acagcggtgt ccttgatgta gtcggtaacg ccggcggtgg
     1921 agcagtgggc gcggtgtgcg gagccaaaag cgtcgtttac ataggcgccg tccgtaccca
     1981 ccagatcggc caggtctttg gagaactcgg gcagattttt ggtttcttcc ttgcggaaac
     2041 gggtgttctg cagcagcaca acatcgccgt cctgcattgc ggcaacggcg gctttggcat
     2101 tttcgcccgc cacattgtcg tcattggcaa acacgacggg cttgccaagc agttcggaga
     2161 ggcgcacggc gacgggcgcg agactgaatt tttcctcggg accgttcttg ggcttaccca
     2221 gatggctgca cagaatgacc cggccaccgt tctcgatcag ctttttaatg gtgggcagag
     2281 cggccacgat gcggttgtca ttggtgatga cgccgtcctt catggggacg ttgaaatcgc
     2341 agcggaccag aacgtttctg ccctttacct caatatcttc aactgtcttt ttgccaagag
     2401 tgctcatatg cgtaactctc cctttcctgc ggctttttga ttgtctttcg gtcttgcgcg
     2461 ggcaatatgc cgcgtctttt cttattatag tattttcccg gctttttcgc aagcattcgg
     2521 ctgatattta attaacaaat cggcgcgtgc aaactgcatg caaacgggct gtaatgcaca
     2581 aaaaacgccc cggcgcaaag ccggggcgaa aagtgcctga attttagaag cagagcgtga
     2641 cgatgcgcag cacgaacagc gcgaacacga cccacatcac ggggtggatg tccttgacct
     2701 tgccgcggaa gatcttcagg aacatccatg tcaggatgcc gaacatgatg ccgttggcga
     2761 tggaataggt gaacggcatc atgatgatgg ccacaaaagc gcccagcacg tcggccatat
     2821 cgccctcgaa cttcaccttc agcacgctgg acatcatgaa caggcccacg atgatgagcg
     2881 ccggagcagt ggcgaagctg gggatggcgc cgaacacggg ccacaggaac agcgagagga
     2941 tgaacatcgc ggccgtggtg agagaggtga ggcccgtgcg gccgccctcg gccacgccgg
     3001 cggagctttc cacatagctg gtgacggtgg aggtgcccag gcaggcgccc gccacggtgc
     3061 cgatggcgtc ggccatcaaa gcgcggccca cgcggggcag cttgccgtcc ttatccagca
     3121 tgttgccctt tgcggcaacg ccgatgagcg tgcccacggt gtcgaacagg tcaacgaaca
     3181 ggaacgcgaa cacgataact gcaaattcaa cggtgttgtt cagcatgaag ctgaagtcga
     3241 atttgaacgc ggtgtcgccc agcgccgcaa agttgggcag gaacgagccg gagggcagca
     3301 gcgaatacac gccggcctcg gggttcaccg cataccagcc cgtgaactgc gcaatgatgc
     3361 ccagccccca cgtgatgagg atgccccaga gaatggcgcc cttgacattg aagtacagca
     3421 tcaggccgat gatgattacg ccgatgagcg cgagcgcgac ctgcggctgg gcgatgtcgc
     3481 ccagagccac cttcgtggag gcgtccgccg cggcgatgcc ggcgttctgc aggccgatga
     3541 atgtgatgaa caggccgatg cccgtggtga tgccgtattt caggttctcg gggatgcagt
     3601 tgaccagcgt ctcacggaac ttgaacgcgg aaagaatgat gaagataatg ccttccacca
     3661 ggatggcggt gagggcgatc tgccagggat tggcctggtc cgccagctcg cccaggcaga
     3721 tggtgtaggc aaaataggcg ttcaggccca tgccggaagc cagcgcgatg ggatagttgg
     3781 ccagaagggc catcacgatg gtggccaccg ccgccgcaag ggcagtcgcc ataaagacgc
     3841 cctggctgtt catgcccgcg tcgcccagca tgctggggtt cacggccagg atgtacgcca
     3901 tcgcaaggaa cgtggtgata ccagccatga cttcggtctt cacgttggta ccgttttctt
     3961 tcagatgaaa gagcttttcc atgatattcc tcctcggtaa ttttaagctg aaacagggac
     4021 gtccccgttc gcaacaaaag aaaaaggcgg gcaaacgagc ccgccataaa acagcaaagg
     4081 ccgcagagct gcggcgtgct gtttcaatag tcgggacggt ttacggcttg cccggtagag
     4141 actgccggcc catatcgccg gcgttatatt gaaacatgtt aacaaactgt gaagaatacg
     4201 tttttattct accgaaatac cgtgacaatt acaatgtgca tttttgccga tttttcgatg
     4261 tttttatccg tgtgtttggg cgaaagggca caagcggagc cgaaaaacag aagtccccgc
     4321 gccggatagg cgcggggaaa gagcggagcg cgttattcgg ccggtgtgcc ggcgttctgc
     4381 ttcggggcgc ggggatgctt ggacggtgcg gcgggcgcgg gctgggcggc atccgcgagc
     4441 ttgccgccca aaaagccgga aagcacgctc tgcaggtcca cgccggtgct ctctttcagg
     4501 ccgtccgtca cctgggtaac ggtctgcatg atgtcccgtg tcagcttggc gctgttgccg
     4561 tcgccgtaca tggtgatttt atccactttc gtcagcggct cggcggcgtt tttcaccacg
     4621 tcgggcaggg cgttgaaata catctccagc acggcggcct tgcccatctt tgtcatggct
     4681 tcggcttttt tctcaatgcc ctcggcctcc gccacagctt tggcacggat ggcctccgcc
     4741 tcagccagcc ccttggcgcg gatgccctca gcttcctggg cacgggcgaa tttttcagcc
     4801 tcggcctgta ccttcatagc ctcggcctgc tgttcctgct cgtatttttt tgcctccgcc
     4861 tcgcgctggc gctcgtacag ctcggcgtct gccttctgct ggcgggcaaa gcgctcggct
     4921 tcggcctttt tccgtacctg ggcatccagg gactgctcca tgacctcggc ttccttctgc
     4981 ttgagcagga tctccttctc ctgcttggcg atgttggctt cagccgtggt gatctcaatg
     5041 gatttgcgct gctgctcctc ctggatgcgg tacgccgcgt cggcctcggc ctttttggtg
     5101 tcggtggcct tctgcagctc ggcctgccgg atggcaagct ccgtcttttt ctccgcgatc
     5161 tcctgtgcgg cggcgacctc ggcgtcgttg gcttccttct gggcgatggc gcgtgccttg
     5221 gcgatctctt tctcactcac ggcgcgggaa atggccgcgt ttttctggat cttgacgata
     5281 ttgtccacac ccaggttctc gatgacggcg ctgtcgtcca cgaagttctg tacgttgaag
     5341 ctgacgatgt caaggcccat ggcggccagg tccggctcgg cgttttcttt caccaggttg
     5401 gcgaatttct ggcggtcgga aaccatctcc tgcaactcca ttttgcccac gatctcgcgc
     5461 atgttgccct ccagaacctc acgggccacg ctggcaatgt agtctacctt gcggttcaga
     5521 aagttctgtg ccgcgaggtt cagcaggtcg ggcgtatcgc tgatcttcac attgacggcg
     5581 gcgtccacct tgatgttgat gtaatccgcc gtaggcacgg cgctggatgt tttgacgtcg
     5641 atggggatga gctgcaggct cagctcgtcc tttttttcaa aaaacggtat tttgacgccc
     5701 gccttgccga tgaggacctt cggcgttttg cgcaggcccg agatgatgta agcggtgtcc
     5761 ggggacgctt ttacgtagcc cgtgacgaag accagcacga tgaacagtac ggcaacggcc
     5821 gcaatgatcc atacgatcac agcaaaaaac ctcctctttt ggtgcattgg atgaaagtga
     5881 aaaccggttc cgggtacaaa aagggcgcgg tgcgtgcact tttccgcgcg cacaccgcgc
     5941 cgccccgtga tccgggctta cgatgctttg gaccccggcg cgttatttcg cgtcggtcac
     6001 ggtctccttg gccgcagcca gcaggccggt caggttctgg atctcgctgg gaatgatgag
     6061 cttcgtggcc ttgccgtcgg caaccttctg cagggcttcc aggctcttga tggccagcac
     6121 cttgtcggtg ggggccgcgt cgttcagcag gcggatggaa tcggccaggg ccttctgaac
     6181 ggtcatcagc gcctgtgctt caccttcggc ctcgcggatg cgctgctcgc gcacggcgtc
     6241 ggcacgcagg atagcgcttt ctttttcgcc ttcggccaca aggatggcgc tcttcttttc
     6301 gccctcggcg cgcaggatgc tctcacggcg ctggcattcc gccttcatct gcttttccat
     6361 ggcgtcctga atctctgccg gcgggatgat gtttttcact tcgacgcggt tcaccttgat
     6421 gccccatttg tcggtggctt cgtccagaat ggccgtgatc ttggtgttga tggtgtcacg
     6481 gctggtgagg gtgtggtcca gctccatgtc gccgatgatg ttgcgcagcg tggtggcgga
     6541 caggttctca atggcggaga gcgggcgctc cacgccgtag gtaaacagct tcgggtcggt
     6601 gatctggaag aacaccacgg tgtcgatctg catggtcacg ttatcgcggg tgatcacagg
     6661 ctggggctta aagtccacga cctgctcctt gagggagact tttttggcga tgcgcgtgac
     6721 aaacggcgtt ttgaaatgga agcctgcctc ccatgtggta tagtacacgc ccaggcgctc
     6781 tacaacatag gaataagcct ggggaacgat ggcgatgttc gcgatgagga tcacgagcgc
     6841 cagcacaatt agaatcagaa acagaatcag catgatagtc ctcctttaaa ttgggtgttt
     6901 tatacgttcc gtcaagacgg aaacagaata cagggggtca taccgccgca gtggcggtgt
     6961 cggggcgcac ggtcagcgtt gcgccgtcca cgctcagcac cacgcacagg gagcccgcgg
     7021 gaatggtttg ttcgctgcgg gcgttccagt ccacgccgtc cagccgtaca cggcccgtaa
     7081 cgtcgggcgt gacctcaacg agaacctgtg ccttgcggcc gacgttgatg tccgcgttgg
     7141 tgggcacacg gcgcgcggtc atgcgctttt tcaccagcgg gcgcgtcgcg gccagggcca
     7201 cggcgcttac caccgcaaac accgcgaact gcaccagcca cgaggaagtg aaccccgaga
     7261 cgatgagcgc agccaccgcc ccggcggcaa accagatgct gacaaggctc accgtcagcg
     7321 cctccacgac gacgaacgcg atgagcagta tcagccagac cagggaagaa attgtaaatg
     7381 ccataatgtg tttacctgcc tttctgccgc gtccgtgtaa cgcagccgtg gttgccgccg
     7441 cgccgcgcac cgtgtacggg cgtacgctgc ggccgggtgt ccggtcgacc ttcgccgcag
     7501 aagccaagcc gggcttttgg ctgtgccgtt tgggtttctg cttgctctta ttgtaccata
     7561 ttcgtcaaag gattgacaaa gcccaaaaaa agccggtttc gcccggtttt ggccttgggg
     7621 ggcgtttttt tcaaagctac tgccccaatc aaacccgggg aacgccgggc gaggccgggc
     7681 cgcgccgccc gaaaaccggc ctgccaggcg cttttatacc tatttatata ggccgccggg
     7741 ggcccggcag gcatgcggaa tgctttcaac ggttctcgcg caggcgttcc cgcgcgccgc
     7801 agcaaccggc ggcttgcggc ggtgcacggc gtagtatata atatcttgta gaattatacg
     7861 gcgtttgggc gagaaaactt tcaaaaacag gaaatctgtg aacaaagggc accgcggccc
     7921 ggcgcacggg aggcataaaa atggaatgca gatattgcgg acaaaggctg aaggacgggt
     7981 acgaatactg cccaaactgc ggtgggcgcg tggagccgcc ggagcagcag gcgcggccgg
     8041 tgcagcccgc atatgcgccg cagggcgcat acagcgcgcc tgtacagggg tattccccgc
     8101 aggaagcgga gggctgtgtc tacggcatgc tccaacccgg cgccgggctg ggccgggtcg
     8161 tttcgcccta ttacgcacgg gaatttgcag acatcgctgc gggcggaaga ggccgtttca
     8221 atgcggcggc attctttctg gggccgttcc atcagcttta ccgcggctgt tacaaacggt
     8281 ttgcacgcac atatctgccc tattggctgg cgctgctggc cgtgctgggc gtgagccttt
     8341 ccttttttgg aaagctgtgt gtggagctgg ccggctacgg cggccgggcc gcagagccga
     8401 cgcaggcgtt tttcgtgtgg atcgccgcga tcctgctgct gtccctgggc gtgagcgtct
     8461 ggggcattgc actgagcatt tacaacggcg tgacctttaa ccgccggtat tttgagcagc
     8521 agcacggtga tccggccgtt ccggcgcata cgggcgcagt cgtcggcgga gcggcagctt
     8581 gggtgggcgt ttatatcatc gccgttgtgg cgctggtcgc agcgctgctt cccgccgcca
     8641 tgcaggatgc gcagcagcat caggcaccgg gcacgctgcc ttatgcacag agcgtgccgc
     8701 aggcgctgcc ggatgacaac gccacctacg gcacggagca ttacggagcg gcggtatcgg
     8761 atgcgctgga agcgcactgg ggcgagggca gcgaccaggc tttgctcaac ggctatatcc
     8821 ccgcacaggc cgcgggcggc acgctggaag aacggctgcg cggttcacac ctgttttaca
     8881 gcgacgcccg cacactggac gagctgtttg cggcggccga ggatgtacaa tggtacacgg
     8941 attacgagcc ggacgccgac ggcaccgaat acgccgacgc ctcatggatc atgggtgata
     9001 cagtgctggt gtgcgtgttt gcagtgtcgg acacatacac gtgggtcgtg gatttttatt
     9061 cgtaccggaa cggcgaggac ccgacagcgg atggctgtta taccttctgg gacgaggagc
     9121 gcagtgcgct gctgctggag ctgtacggtg aaaacgctcc ggaagagccg tcgctggcgc
     9181 ggcagatgcg cggcgcgtgg gtagatggcg caggcggggc cgttctgctg gatgaatctt
     9241 tttttgacgg aaccgattat acgctgggca cgatgatgga cggaggcgta caatgctggg
     9301 tgcaggacga cggctcctac atcgtgttcg tgctggacga gacaggcaac gcgctcaccg
     9361 cgcgctggta cgacggctcc ggcacgctgc gcgccgagca gtcctttacc cggggcacgc
     9421 cgggctgagg atgaacggag cagggcaaat gcaaaaaccg cgtcaggttt tccctaacgc
     9481 ggtttttgca tattatgagg ccggatgtat tgtgaaaagc tcattccggg tcggtcacaa
     9541 cggcaaagct gcagggctgc acttcgacgg tacgtcccag aagcacggtg cgcacctgct
     9601 gttcggtccg gttgatgcac acggcagcca cggcggcttc ggcgcggcgc tcgtagcaca
     9661 gcacgccgcc ctgtgcctgg gtgacggcca gcgttccgtc ggcaaaagcg gggcagcttt
     9721 tgcgcagggc ggcaaggttt tgcagcacgg ggcggatgcg cgtttcccga ctttcccaat
     9781 cgaaaaaggc acggttgaac gggtccttgt agccctgcat gccgatctcg tcgccatagt
     9841 agatgctggg gatgcccggc agcgtaaaga tcatcgcata ggccagcgtc atcaggcggc
     9901 ggccgttttc atactgctcg ggcgtcaggc ggcgcgcgct ctgccagtat cggtcacggc
     9961 cctcgcagct ctcgcccgcg atggcggtaa tggcgcgcac ggtatcgtgg gtggacatga
    10021 agttcatcag cacatgcagt gccggggcgg ggtagtgctc gcagatacgc atgatgtcgt
    10081 gggcggctgt gcgggcgtcg ccgcctttca caaaagccag cgtagcgtcc ttgaacggat
    10141 aattcattac cgcatccagg cctttgccca gcaggtaggt gcggcggcgg ccgtagctcc
    10201 acttgttggt ggcgtcctcc cacacttcgc ccagcagata tttttcgctg ccgtgacgct
    10261 tgacggcgcg gcggatctcc tcaataaaat catcgggcag ctcgtcggcc acatccaggc
    10321 gaaagccgga agcccccagg ccgagccaat agtcgatgac gccgccctcc ccgcagatga
    10381 actcacggta ggagcggtcc tcttcattca cctcgggcag cgtttcaaag ccccaccagc
    10441 agcggtagcc gtggggatag ttcggtgaaa aatcgtacca gcggcggtac gggctgcccg
    10501 gtccctgcca tgcgccgaag gaggcgtagc ggccctcgcg gttgaagtag acggaatccg
    10561 aaccggtgtg gctgaacacg ccgtccagca cgacgtcgat gccgcgggct ttggcggcgc
    10621 ggcacagctc tgcaaaatcc tcgttggtcc ccagaaccgg gtccaccttg cggtagtccg
    10681 cggtgttgta acggtggttg gcgtgggctt cgaagatggg gttgaggtag atgcatgtca
    10741 caccaaggct ggcgaggtac gtcagctttt tgcggatgcc aggcaggtct ccgccgtaat
    10801 agtccatgtt caaatagccc tcatgctgtt cgtttggcca gaaatagggt tcactgcgct
    10861 tgtccgggcg gtagatgcgg tcggcatagg gcatggtagc atgagggctg ctttcaaaga
    10921 agcggtccgg gaagatctgg taaatgacgc cgccgcgcag atggcttgga gtctgaaaat
    10981 ccttttcgta taccgtcagc tgccaggcct cgccgtcgcc tgttgtaaca tatgcctcgc
    11041 caagctgccc ggcgtatagt ttgcggtagc cggtgtaaag gtcaaaataa tagaaataga
    11101 gccctggctc ctgcggctgt atcgtagtgg agaacacgtc cataccgttg cggaagtatt
    11161 gtttttggag cgggaacagg gacggctgtt cgccgtccct gttgaaaagc agatagggtg
    11221 tggtgcagcc gaactccacg ggaaccgtca gcgtaaaggc aacggtctct ccggcgcgga
    11281 ccgcaccgaa aggggtcttg taaaaagtgt cctggctgtt gaaaatggtc acgggcattc
    11341 cgattgctcc catgcagttt tatttggcgg gcgctggtcg cgtccgcaga catccgggcg
    11401 gaaacgcacg gacggatgcg gcgggcggat ctatttgatg ggctttacgt gccagatatc
    11461 gcgggcgtaa tcgttcaccg cccggtcggc tgaaaaaatg cctgcgttgg caatgttcat
    11521 caggctcatg cggttccaca cgccgcggtc tcggtacagc ccgctcaggt cgttctgggc
    11581 gcggcggtaa tcggcaaaat cggccatcac catatatggg tcgctggtgc gcaggttgtt
    11641 cacgatctca tggaagctct cgccgcccca gccgcgctcc agaaatgcca gtacttcggc
    11701 ggcctcgggg caattgttga tgaagccgga gggatggtag ccgaagcgct tcaggtcgtt
    11761 cacctcgggc gtcagcatgc cgaagatgat ctcgttttcc ctgccggcgg cctctgcgat
    11821 ctctacattc gcgccgtcca gcgtgcccag cgttaccgcg ccgttcagca tgaacttcat
    11881 gttgcccgtg ccggaggcct cggtgcccgc aaggctgatc tgttcactca cttcg
//
LOCUS       DAWBSB010000044        11914 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1270_length_11914_cov_2.632094, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000044 DAWBSB010000000
VERSION     DAWBSB010000044.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 11914)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11914)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11914
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1270_length_11914_cov_2.632094"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2551..2560
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5078..5087
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8893..8902
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2351 a         3402 c         3772 g         2359 t
ORIGIN      
        1 cctttggcat tttatcctcc taaaaataaa aacaaatgca taaacttgtt tttattttac
       61 catatacaat cgaaaaaaac aattcagagt tttttgaaac aaagaagaag ccggagctaa
      121 aagtagaagc ggctcagccg atgccgccgc aaatgatgct taaaatgaat accaacacgg
      181 ccagccctac gtaaaggtac cgcgccgtgg gcaggaacgc ccggcacagc gcttttttgc
      241 ggcccagcac aagctcttct ccgatgcgtt tgccgcccag cacccagtag atgatgaacg
      301 cgcacagcag cgctccgaag ggcacgacat aaatggtgat ggcgtccatc catttgccca
      361 ggtacggctc aaattcaata aacgcgcccg ccgcaaaggc caacgcgccc accagtacaa
      421 cggcggcttt gcgcggcagc ttcaggtgtg tcgtcactgc ctcggcgcac acctcaaaca
      481 tattgatgag gctggtgata ccggcaaaaa taacagagag gaagaacagc acggcaatga
      541 agcgcccgcc cggcatctgt gcgaatacct tgggcagcgt gatgaaaata agcggagggc
      601 ctgacgcagg atccatgccg aaagcgaaca ccgcgggaag gatggcaaag ccggccagca
      661 gagccgcgca ggtgtccagc agcgccgtct gcatggcggc gcgggggata tcctctttct
      721 tggagaggta gctgccgtag atgatcatgc ccgagccggt gatggacaga gagaaaaacg
      781 cctgtcccat ggccatgacc catgtcatcg ggtccagcag cttttcccac tggggaatca
      841 gcagatagcg gtagccctcg gcagcgccgg gcaaaaaacc cacataaaca gccacaacga
      901 gaaacagcac gaagaaagcg ggcatgatga tgcggttcac cttttcaatt ccctgcagca
      961 cgccgcgcgc caggaccagc accgtgacga gcaccaccat gccgtgccac agcaggctgc
     1021 cgaacggccc ggtgatctgc ccgaaatatt cctcggatcc cacggcaaac agtgaaccgg
     1081 agacggctcc ccagcagtaa cgcaccaccc atcccacaat gacggcgtat ccgagggcga
     1141 tgcccagcga acccagcaaa ggaatggcgc ccagcacgcc gccgcctttt ttgccgcggc
     1201 tgcgcagcgc gtaagcgtag gagccgatgg gccccgtgcc cgtcagacgg ccgaaggcga
     1261 attcgccgga aaggcccacc catccgaaaa gggcaacaaa gccgaaataa atcagcagga
     1321 acgccgcgcc gccgtactgg cccacgcggt aagggaacat ccagatgttg cccatgccca
     1381 cggcggagcc gacagaggca aggacgaagc cgatgcggtt tttgaaattg gcaggttggt
     1441 tctgctgcat gagaaagcgt tcctttcagc cgctgcggag ctgcgccctg cggcagtgtg
     1501 atgtcattca gtatatcata aaacaggatg cttcccaagc cttttttgtg tttttaccgg
     1561 catgagcggt gtaaaaaagg ccgctgcatt tgcagcggcc cgtaaaaacc aaatgggaaa
     1621 tcttcaaagc gcgtggttac gcaggctgtt tttccgcgcg gcggcgtgca gccttacgct
     1681 ggaagaactt gacgcattcc acaatgggga tcacacacac ggccagagcc atggcaacag
     1741 catattccgg cgcgctgatg tgcgtaaagc cgaacgcgtc cgaaatgccg ggcaggtaga
     1801 tgacgccggt cgtcagcaca aggctgagca gcatggcgcc ccacagcacc ttgttgtgag
     1861 agccgagcgt gaacacgctg ccgcgctggc tgcgcatgtt gaagctgtgg aagatctcgg
     1921 ccatgctcat ggtgagaaag gccatcgtca caccgtcggg gctggcggcg atctcccata
     1981 cgccggattc catccagtgg ccgataaagt aggcggccag cgttacgcag gtgaccataa
     2041 agccctgata cgccacgtct acgcccagcc cgccggagaa gataccgtct gtggacgggc
     2101 gcggcttgcg gtgcatcaaa tccggctcgc ccttttcaag gcccagggcc agagcgggga
     2161 agcagtcggt gatgaggttg atccacagca ggtgcacggg ctccaggata acaaagccca
     2221 tgagcgtggc aaagaacacg ctcagcacct cgctcatatt ggagcccagc aggaactgga
     2281 tggccttgcg gatgttgtcg taaatgcggc gcccttcctc cacggcgttg acgattgtgg
     2341 cgaagttgtc gtcggccagc accatatcgg ccacgttctt tgtaacatcc gtgccggtga
     2401 tgcccatgcc gatgccgata tccgcgcttt tgatactggg cgcgtcgttg acgccgtcgc
     2461 ccgtcatggc tgtgacaaag ccgtttttgc gccatgcgtt gacgatgcgc accttgtgct
     2521 cgggctgcac gcgggcatac acgctgtaac nnnnnnnnnn gtaacggccg atcacttccg
     2581 caaaccggtc gtcggagatg tcgttcagct ccgcgcctgt gatggcttca tccgctgttt
     2641 cgatgatgcc gagctgcttg gcgatggcga tggcggtatc cttgtggtcg cctgtgatca
     2701 tgatgggacg gatgccggct tggcggcact ctgtaatggc gtcctttacc tcggggcgga
     2761 tcgggtcgat catgcccgtc aggcccagga aggtcaggtc atgctccagc gtgtccggct
     2821 cgaaatccgg cggaagctgg ggccattcgc ggctggccgc gcacagcacg cgcagcgctt
     2881 tgtcggccat ctgcttgttc tggcgcagga tctcggcgcg ggcttcctcc gtcatgggca
     2941 gtatcttacc atccttggcg taagaggtac agcggctgag caccacgtcg ggcgcgccct
     3001 ttgtgtattg gatatatgcg ccgttgtttt cgtgcacggt gctcatcatt ttgcggccgc
     3061 tgtcaaaagg agcctcgccc acacggggct gggccgcctc cagcgtgccc ttgggcaggc
     3121 cgacgccggt ggcgtaattt accagcgcgc attcggtggg ctcgccctca gcgtcgccgt
     3181 tttcgccggg cttggcgtcg ctgcacaaag acatcgcgcg ggccaggggc tcttcttcac
     3241 ccacgtgctc cacaaccgtc atcttgttct gcgtcagcgt accggttttg tccgagcaga
     3301 tgacctgtgc gcagcccagc gtctccacag ccgtaaggcg gcggatgacg gcattgcgct
     3361 tggacatgtt cgttacgccg atggagagta cgatggtaac gacggtggca aggccctcgg
     3421 ggatggccgc gacggcaaga ctgaccgcta ccatgaacgt actgatgatg acgtcgaggt
     3481 ggaaatcgcc cgccttgata aggctgaagg cgaagatgaa tacgcagatg ccgagcacca
     3541 gccagctcag gatcttactg agctgcgcca gctttttctg cagcggagtc tgtccctctt
     3601 cggcctgcgc cagcgcgcct gcgatcttgc ccatctcggt gtccataccg gtgcccgtga
     3661 tgaggacacg tccgcggccg tatactacgg tgctgcccat ataggccatg tttttgcggt
     3721 cgcccagggg gacgtccttg ccgtcgtcga ggcccagtgc ggaaacgact ttgttcacgg
     3781 gtacgctttc gcccgtgagc gcggcttcct cgatcttcag gctggcgctc tccagaatgc
     3841 ggccgtctgc gggcacggcg tcgccggctt ccagaacgac aacgtcgccc ggcaccagct
     3901 cctcgctttt cagcgagacc tgtttgccgc cgcggatcac cttactggtg gcggccgtca
     3961 tttcctgcag cgcctcaatg gctttttccg ccttgctctc ctgatacacg ccgagcacgg
     4021 cgttgatgag cacgacgata aggatgatga acacatccgc aaagctttcg cccgaataag
     4081 ccgccgttac accggacact gccgccgcaa caagcaggat aatgatcata gggtcgctca
     4141 gctgctgcag aaagcgctgc agcgttgtga ccttttttcc ttcggccagc ttgttggggc
     4201 ctttttcggc cagccgcctg gcggcttccg cgtcggtaag gccgtccggg gtgctcttca
     4261 gttcggcaag cgcccggccg gcctcctcac aataaaactt caacacacaa catcctttct
     4321 cgtttcaagg gtttcccaat ttggtgtttc tatgaaaaaa cagcaagctg tttttcatcc
     4381 gcggaacatg cgccgagcgc ccggcatggg ggaacaacgc aaaaaagcca cgcacaatac
     4441 cagaccccgg caaaatgcag gggcggcctg tggggcatat ccctgtccgt caagaggcct
     4501 ggataagaaa aaagacccac gtgcggcttg gcgccacaca tgagtctcgt tcttactttt
     4561 aagccaggtc cgtgtgcggg ccatgctgtt gacttaaaac cgccccggcg gggcgtgaac
     4621 tactccctca tggagaaaag aaaaagtttc ctttgttcaa tattatacac cagctttcgc
     4681 cgcttgcaag tccctgtttg taaaggaaac gttaacaaaa gacatttttc gcacgttttc
     4741 cgccggaaaa ctcaggcttc gtgcgcggtg ggaaatattt gaaattgaac gcatgcagtg
     4801 ttatgatagc atcaacgatc aaggaggcaa acatatgatc tatacagtta cattcaatcc
     4861 ggccatcgac tatgtggtgc agctggagca cctgacgcaa ggaactgtga accggaccgg
     4921 cgatgaggcg gtctattgcg gcggcaaggg gatcaacgtc tccacggtgc tgacgaacct
     4981 gggcatagaa aatgtggcgc tgggcttttt ggcgggcttc acaggcgatg ccgttgaaaa
     5041 cgcgctgcaa agaaaagggc tgcgcacgga ttttatcnnn nnnnnnngat tttatccgtc
     5101 tgcaaaacgg gatgacccgc atcaatgtga aaattcgcgg cgcctgcgag acggagatca
     5161 acggcaaagg gccggacata ggcccggcgg cgctggcgct gctgtacaaa aagctggacg
     5221 ccgtcacgga cggcgatgac cttgttctgg cgggcagtat tcccgcgggt atgccgggcg
     5281 atatctatga gaagatcatg gccgcgctgc aggaaaagaa ggtgcgcgtc acagtggatg
     5341 ccacagggga tcttctgctg aatgttctga agtaccgccc gttccttatc aagccaaacc
     5401 agcacgagtt gggcgagctg ttcggccgca cactgaaaac cggggatgat atccgtgcat
     5461 gcgccgcaaa gctgcaggac aagggcgcgc gcaacgtgct cgtttctctg gcgggcgacg
     5521 gtgcgatcct gctggacgaa acgggtcagt tccaggagat cggcgttccg cggggcacgg
     5581 tgcgcaatgc agtgggtgcg ggcgattcca tggtggcggg ctttttgacc ggttacctca
     5641 agctgggcac ctattcctac gctctgcgca tgggcgcggc cgcgggcagc gcgacggcgt
     5701 tttccgatga gctggccaca aaggaagaaa tattgaagct gctgaatacc ttttaagcgg
     5761 aataccggga aagggaagag gaacatgttg ctgcaaggcg taggcgtctc ccctggtgtc
     5821 ggcatcggcc gggccgtggt tattgcggag gggagccttg attacacaca ggtccatttc
     5881 gcgggcgtgc agcaggaaaa agcacgcctg cgcacggccg tgcaggcatt tgcacagcgg
     5941 acgcaggcta tggcggacgc gctgcgcgcg cgcctggacg gacaagaggc ggacatcctc
     6001 gccagtcaga ttctgatggc aaacgatccg gctctgcttg cgcagacaga cagccgcatt
     6061 gaagcgggcg aatgcgcgga ggccgctttg gacgcagtgt gtgcgtcata tatccagctg
     6121 tttgccgcgg caggcgacga attgctggcg cagcgtgccg cggacataga ggatatccgc
     6181 gcccgcctgc tggcgatttt gctggggcgc agtgaagcgt ccatcccgga cgtgcctccg
     6241 ggcaccgtgc tgattgcatg ggatctcacg ccttccatga ccgtgagcgt gaaccgggaa
     6301 aatgttgccg cttttgtggc ggaatgcggc ggcatgacga gccatgccgc cattctggcg
     6361 cgtgcgttgg gccttcccgc cgtactggga gccgcggggg ccgcgtctgt gctgcggagc
     6421 ggggaggatg tcgtcgtgga cggcgggcag ggcgcggtga tcgtatctcc aagcggggcg
     6481 gagctggcgc ggtaccggaa aaaacaggag gaggagaaaa gacaggcgga ggcgctgcag
     6541 gcgtaccgcg cgggcccggc ggtgagcgcc cgcggccaaa ggtacgcgct gtacgccaat
     6601 atcggcggcg tggggcagac cgctgcggcg gatgaagccg gggcggaagg agtcgggctg
     6661 ctgcggacgg aatttctgtt tatgggacgt acatccttgc ccacggagga ggagcagtac
     6721 gaagcttatg catcggtgtc ccgcgctatg ggggacaggc ccgcggttgt ccgtacgttg
     6781 gatatcggcg gcgacaaggc gctgccgtat ctgggacagg aaaaggagga gaatccttac
     6841 ctgggccgcc ggggcatccg ctactgcctt gcccggcctg aggtgttccg cacgcagctt
     6901 cgggcgctgc tgcgcgcggg ggcggagcgt gggaacatca ggctcatgct gccaatggtg
     6961 acgggcgtac aggagctgcg cgctgcacgg acgatgctgg agcaggaaaa gcaggcgctt
     7021 tccgcgaggg gcgttgcatt tgacggggcg atttctctgg gcgtgatgat cgaaacgccg
     7081 gccgctgccg tcattgcgga tctgctggcg aaggaggcgg attttttcag tattggtacc
     7141 aacgacctgg tgcagtacat tttagccgcg gaccggggca atgcagacat tgcggagcta
     7201 tatgcaccct gccatccggc ggtgctgcgc agcattcggg atgtgatcgc cgcgggccgt
     7261 gcggcagatg tgccggtgtg catgtgcggc gaagctgcag cagacccgtg tcttattcct
     7321 ctgtggatgg cgtttggcct ggattctttc agtgctgggc cctccgctct gccggaagtg
     7381 cgccggactg tttcacgctg gacggaggaa gaagcgcttt gcacggcgaa ggaggcactg
     7441 gcctgtccgg atgcggatgc tgtgcgggcg ctgctggccg cgcgccgcag atagcggcaa
     7501 agcggcgggg agcaggattt gcacaaattg cggaaatccc gttcccacat gccattcttg
     7561 cggttttgcg cctgcccgct tgacggggcg ggcgcttttt tctacactgg aatacaaaaa
     7621 gacgggcgcc gcgccaaaag cgccgcaccc atagctgcgg gccgggaaag gcctgcggca
     7681 ttactttgcc tggaggatgt tttatgaatg ctttccggca aaacaaggcg gcctgcgcat
     7741 atccggccat gctgctcaat ggtgtgtgcg ccagcagcgg cggagtggtg atggcgcttt
     7801 tgcaggaacg gtacggcctg agctaccagt ggagcggcct gctgctggcc atgctgagcg
     7861 tgggcaacct tgcggcgggc tttgccgcgg gcatgctgcc cgcgcactgg ggccagcggc
     7921 acaccgttct tatgctggcg ggcggtgcgg cgctgggtta cggcgcaatg atcctcacgg
     7981 gaagcccgtg ggcgctgctg gccgctttca cattggtggg cgtggccaag ggcacaacgc
     8041 tgaacaccag cagcgcgctt atcgcggcgc atgcacggga gcgcacgaaa tgcatgaatc
     8101 tcatccatgc atgctttgcc acggggtcgc tgctgtgccc gctgtttatt gcggcgctgg
     8161 gtatgggcgg gctgccctgg tgggcgccca tggcaggcct tgcactgtgc ggcgcggcgc
     8221 tgtggggcgt gttcgcgttg gcgggtctgc cgggccgcac aggagacggc gcgcgtgtca
     8281 aaaacgactg gtcttttctg cgttcgcccc gtttttgggt gctgaccggg cttgtctttt
     8341 ttcaaaactg cacggagatc agcgtcacgg gctggatggt gacatatttc aaggatgtgg
     8401 gcatcctctc gggcccggcg ggccagatga ccgttacggt catctggggc gcaatgctgg
     8461 ccgggcgtct gtgcatcgca tttgttttcc cggtaaaaaa cagcttccgc accctttctc
     8521 tgatgtccgc atgctgcgta ggcacctata tcctgcttct catggcaaaa acgcccgcat
     8581 ctgcgctgat ctgcctgttc ctgttcggcc tggccattgc aggcgtgaac ccgacggctg
     8641 tcgcggcggg gaatgcgctc tccggcgcgg gcctcggcgt gatgcttccc gcagcgggcg
     8701 tgggcgcggt tttaatgccg tacatcacag gcgcggtggc gcagcggttc ggcatctggg
     8761 caggtatgct gtgcacggtg tttgcgctga tgggcatgac ggcatgcgcc gttgcgctgc
     8821 accgcagaaa cgatatgaaa taaacgtgcc gcaccggcgc ttgaaaaacg gcggttgccg
     8881 ccggatgcaa aannnnnnnn nncgctgctg tattcgtgga aagagcggtg cggcgttcac
     8941 acgctgccgc cctgcggcag aggctcggtg ccgaaatagg cgcagatcgc ctcgtaatag
     9001 atacgtgcgg cgcgggcgca tccggcctct cccgtccagt tttccacatc gctgtaattg
     9061 gtcagaaaac actgctccac cagcacggcg gggcagtagg gcctttccac gatgccgaag
     9121 ctcttttgct cgcgtatctt ggtgtcggtg ctgtccacga tgcgtttgct ctttccgttg
     9181 taataggcga aacgaatgcc gttttctccg cgcaggcggt gcccggcgcc gcccattttc
     9241 tgcgcgatgc attgggcaaa gcgcatggat tcttccgagt aggtgcgccc gggcggcgtg
     9301 gggaagcatt caaagccgtg ggactgtccc gtgctttcgt cgctgttggc atgaacgctc
     9361 agaagcaggg aagcgccggc gtcggtcgcg gcctgtgcac gctccttgat ggagcggtcc
     9421 tcgccgttgg ggcgcgtgcg cacaggcgca tagttggagt cctgctccag aagcgtatag
     9481 agggcgttta ccgtctgttc gcatacttcc agttctttga tgatcgcctc cgcaccggtg
     9541 tcgaagccgc cgtgtccggc atccagcgcc acggtgtagg gcggctgggt ctgcagcaga
     9601 tacggccggt ccgggtcgcc cgtgagttgg ggcggtgaag gcgatgcagg cgccgggctg
     9661 cggggcgtgc ccgaaaaacc ggcaggctcg ctgagcaccg tgtgcatgca tgcggccatc
     9721 agcaggccgg caaggcatac cgctccaagg caaagcgcgc gcctgccgcg ggtgtgcagg
     9781 ctgtatacgc cgccctgcct cgcagagcgc gggccgcggt ctgcggaagc gcgtgcgggc
     9841 ccgctcatgc ggcaggcgcg gcaggcggcg cgccaagttc tgaaagtgtg ccgaaggtat
     9901 accccatctc ttcccaccgt gtcagcaatt catccagaat tgccgcattg gtggcgctgg
     9961 tggaatgcag gagcacgatc gcgccgtcgt gggtacgggg catcagtttg tcaaaagcgg
    10021 cctcccgcga aggctgtgca tcctgtttcc agtccacata ggcgaggctc cagaaaatgg
    10081 ttttgtagcc cagttcctgc gcccaggtaa ggttttcatc gctgaatttg ccttcgggcg
    10141 gtcgatagaa aagcggcagg ggcgttcctg tcgtttcctg gaacagcgcg gcaagatcat
    10201 tcagctcttt tttgaaagag gcttcatctt tttgggacat gttcgggtgg tgataggtgt
    10261 ggttgcccac cgtatggcct tcggcgacca tccgcttgat caggtccggg ttttccttga
    10321 tatagctgcc tactacaaag aaggtgccgg gggcgttgtg ctttttcagc gcgtcaagaa
    10381 tgggtgcggt attgccgttc tcataccctg catcgaacgt cagatacagc cgtttctgct
    10441 ccgcgtctcc gcagtaaaaa gcatcgaagg ggcgcagctt ttcggctgag aggttgggaa
    10501 cgggcggttc gttttccgtc tgaaacgaaa ggccccattc ataagaagca gcagccacag
    10561 ccgcgggttc tgcctccgcc cgcggccagc cgagccccag cagaacgccg cccgccgccg
    10621 cagcgcacag cacggcagcc agtacgcggt gggccgcttt tttcgggatg catagataca
    10681 tctttgttca cctgccttgt ggtgcaatca tatgcgggcg caaaaagaaa aatgtaacgg
    10741 cggacagatt ttgttcgccg tgcacaccct gtgacggaaa agaatgagcc gccgaatatt
    10801 ttgcagagcg ctgtgccccg tggagaggca tacacgagaa aatacagcgc ttcggttgac
    10861 aagtgaaaac gccggtgcta cgataaatcc atatctatta tgcgaggtgg aagaggtgcg
    10921 gcattcggta cggcacatgg cgcttttgtt ccacagaaag gaaagaaaca tggaaatcaa
    10981 atacacgcag acagaaaaag cacccgcggc catcggcccg tactcgcagg cggtgtctgt
    11041 cgggaatctg ctgttcactt ccggccagat ccccattgat ccggccacgg gcgagatcgc
    11101 gggtgcgacc atcgaggaac aggcgcgcca gagcgtcgcc aatgtcgggg cgattctgga
    11161 agccaacggc acttcgtttg acaaagtggt gaaaacgacc tgtttcctgg cggacatgag
    11221 cgactttgcg gctttcaacg ctgtgtatgc tgaggcgttc gtgtcacgtc ccgcacggtc
    11281 ctgtgtggcg gtgaagacac tgcctaagaa tgtcctgtgc gagatcgaag cgatcgcggc
    11341 actgtaaagc atataagccc tatgccaata ggggcaaaat aagggaaaca cccggcagac
    11401 cgtttcggct gccgggtgtt ctgcatttta taatatatgc ggccgctccg tttccgtctg
    11461 gagcagggcg cgcagtgttt tgcgcacatg ctccaggtcg gggtcgcaca gcagcggcag
    11521 aagctccgtc acctgctgcg cgggaaggtc ccaccagcga aactgcagca aaagcccggt
    11581 cagctctgcg tcaaagcggt gcttgagcag ccttgcggga ttgccgccca cgacggtgta
    11641 aggttctacg ttccgcgtca caacggaata agccgcaaca atggcgccgt caccgatttt
    11701 tacgcccggc ataacggtgc attcccgccc cagccataca tcgctgccca caacggtatc
    11761 gcccttgaat gggagctggg acaggtgcgg cggcgtactt tcagcccatg cgccgccgaa
    11821 tacattgaat gggtaagtgg tgacgctgca catccggtgg ttcgcgctgc ccataatgaa
    11881 gcgcgcgccg ctcgcaatgc agcagtactt cccg
//
LOCUS       DAWBSB010000045        11859 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1278_length_11859_cov_3.016012, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000045 DAWBSB010000000
VERSION     DAWBSB010000045.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 11859)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11859)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11859
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1278_length_11859_cov_3.016012"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    631..640
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1421..1430
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2136..2145
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2483 a         3303 c         3919 g         2124 t
ORIGIN      
        1 gaataacagc ggtaaaacgg aaacggtatg aggggaacag gaggataaaa atggcggaac
       61 tgatcagcgg aaaaacactg gcggcaaaac tcaaggcgga ggccgcggca gaggcggcgg
      121 agctgcggga aaagggcgtt cacgcccgcc tggccgtcgt tttcgtgggg gagaacccgg
      181 ccagcaggac atatgtgtcg ggcaaggcga aggactgcgc cgaatgcggc ctgctgtccg
      241 acgtcatcgc gctgccggaa gaaaccaccg aggccgcgct tctggaaaaa atcgcggcgc
      301 tgaacgcgga caagacggtc cacggcgtgc tggtgcagct gccgctgccc gggcatatcc
      361 gcgaaaagcg cgtcatcgac gccatctcgc cggacaagga cgtggacggc ttcaccccag
      421 tgaacgtggg ccgcatggtc attggtgagg agtgctttct gccctgcacg cccgccggct
      481 gcattgaaat gctgaaaagc accggcgttc ccatcgcagg caaggacgct gtggtcatcg
      541 gccgcagcaa catcgtgggc aaacccgcgg cgctgctgct cacggccaac aacgccaccg
      601 tcacgctgtg ccattccaaa accagggatc nnnnnnnnnn ggccaaatgc gccgcggcgg
      661 atatcatcgt ggcggccatc ggcaaggcgg gcttcgtcac cgccgatatg gtgaaggagg
      721 gcgccgttgt gatcgacgtc ggcatcaacc ggaacgctga gggcaagctg tgcggcgatg
      781 tggactttgc gcccgtggcg gaaaaagccg cttacatcac tccggtgccc ggcggcgtgg
      841 ggctgatgac gcgggccatg ctgatgaaaa acaccgtcaa ggcggcccgc ctgcaaagcg
      901 ggctgtgagc gcgcggcaca tggggcggaa aaccgcgccg caccgcgaaa aaaacacttg
      961 catggggcaa atatttgtgg tatagtatta aagccgcatg gtttgggcat ttgaagcggc
     1021 gcgtctgcgc ccgcctacta gtccagcgag acttatacaa atgagggtaa attatgtacg
     1081 cagtgatcgt gaccggcggg aaacagtacc gcgtggaaaa gggcgacgtc atttttgtgg
     1141 aaaagctgaa tgccgaagcc gagagcgaat tctcttttga caccgtgctt gcggtgggcg
     1201 aagagggcaa tgtgaagttt ggcaacccca ctgttgcggg cgccaaggtc acaggcaagg
     1261 tcgtaaaaaa cggcaagggc aagaagctga acatcctgac ataccgcccc aagaagggca
     1321 gcatgcgcaa gatgggccac cgtcagccgt atacaaaaat cgaaatcacc gagatcgtcg
     1381 gttaacaggg gagggcattt gaaatggcac ataaaaaagg nnnnnnnnnn ccgacggcca
     1441 gtttgtgctg gccggcaaca ttctggtccg tcagcgcggc acccacatcc atccgggcga
     1501 gaacgtgggc cgcggcagcg acgacacgct gttcgccctt gccgacggca aggtctgctt
     1561 cgagcgcgtc ggccgcgacc gcaagaaggt cagcatcaag caataagcct tgcagaaggc
     1621 agtcggccgg gcccgtgttg gcccggcctt ttttgataat caacgggaat ggtgaaacgc
     1681 taaaggtacg cccgggagcg ggctttggct tttccgcccc gctcagcggc aatcagatgc
     1741 cggaggtttt ttatgttcat tgataccgca aaaataaaca tcaaagcggg caacggcggc
     1801 aacggctcgg tgtcgtttca ccgggaaaag tatgtggccg cgggcgggcc ggacggcggc
     1861 gacggcggac gcggcggcaa cgtggtgttc gtggccgatt ccaatctgtc cacgctgatg
     1921 gatttccgtt acaagcgcaa atatgccgcc cagaacggcg aggacggaag agccgcccgg
     1981 tgcagcggca aaaatgcgcc ggaccttatc atccgcgtgc cccgcggcac gctggtgcgc
     2041 gacgcgcaga gcggccttgt catcgcggat atttcaggcg acgagcccgt ggtggtggcc
     2101 cgcggcgggc gcggcggctg gggcaacgtg catttnnnnn nnnnnatccc gaaatttgcg
     2161 aagcccggcg gaaagggcga ggagctggag gtgcgtctgg agctgaagct gattgcggac
     2221 gtgggcctgg tgggctttcc caacgtgggc aagagcacca tcatttctgt catcagcgcg
     2281 gccaagccca aaatcgccaa ctaccatttc accactcttt cgcctgtgct gggtgtggtg
     2341 cgcgtagggc cggaagccag ctttgtggct gccgacatcc cgggcctcat cgaaggcgcg
     2401 gccgacggcg ttggcctggg gcacgacttt ctgcgccatg tggaccgctg ccgcctgctt
     2461 ttgcatgtgg tggacgtctc cggcagcgaa gggcgcgacc caaaggacga ttttgaaaag
     2521 atcaataccg agcttgccaa cttttcgcag gagctgtccg cgcggcctca aatcgtgctg
     2581 ggcaacaaat gcgacatcgc gtcgccggag cagatcgcgg agttccgtgc gttcatcgag
     2641 gaaaaggggc tggtgttcct gcccgtctcc gcggctacgc ggcaggggct ggacaagctg
     2701 ccgggcgttg tatatgagcg gctgcagcag ctgccgcccg tcaccgtgta cgagccggag
     2761 tacgtccggc ccgaccctgc attgaaagac ccccgtgcgt tcgaaatcgc ccgcgccgac
     2821 gacggcgcgt gggacatcga cgcgccctgg ctggaaaata ttctggaagg ctcggatgta
     2881 tccgactacg agagcctgca gtatttccag cgccagctca tggattccgg tgtcatcgac
     2941 aggcttgtgg agatgggcgt gcaggaaaac gacaccatcc gcatcgggga atttgagttc
     3001 gattatgtat tttgaccgtg gccgagcgga aaaacgggcc gcctgagcgg cgctccgcgg
     3061 cggaatggaa aacaggcagg gggagacggc cgcagtgtta tttgaagcaa agccggagat
     3121 agacatccat gagcagtcgc ccatagcgct ggcgttcgtg ggagacggcg tgtttgaggt
     3181 gctggtgcgc gcgcgccttg tggaacgcac gcgccttgtg ccgaaccgcc tgcatgcggc
     3241 ggcggtgaaa tttgtgtccg ccaaagggca gtatgcggcg ctggaggtca ttgcgccgct
     3301 gttggacgag gccgaacggg ccgtggtgcg ccgcggcaaa aattccacaa aggccactgt
     3361 ggcgaagcat gccaccccac aggagtaccg ggcgtccacg gcgctggaat ccctgtttgg
     3421 ctggctgtat ctgcagaacc ggggcgcgcg catcgaagaa ctgttcgatg tgatctggaa
     3481 tgcgctggcg cccggtttcg cgctgcaggc ggaatgaaaa caaaaagggg ctgcggcgta
     3541 tttcccaaac gcataaaacg ggtttggaaa acgcgccgca gcctttgcat tgggccgccg
     3601 ggattattta cactgggtgt tgcccttgcg gttcgtgctg ttcgagctgt tcttcgcgtt
     3661 gctggttttg tcagagctgc ggtcctgggc gttgctgctc ttggcgttgc agtcccggga
     3721 agaactggtc ttgttgctgg ccttgttgga agcattgctg gccttgtttg tgctgttgga
     3781 agacttgttg ctcgcgttgc gggcgttctg cgttttgttt tccatctggt ctcacctccc
     3841 ttgctgatac tattattcac agggagcatc catgatattc gcggaccgat acggtctttt
     3901 tttgatttac ggcgagaggg aggatgcctg tgccgggaga atattttgaa gcgcgggagc
     3961 gcgcgctgct gggcacacgg tacgacgtgc tgtacgccgc gccgtgcgcc gtgcgccgcg
     4021 gcgttacggt aaacggcctg cgctgtgcgc cgcaggagtt cgccgcggcg gtggatttcg
     4081 gtttggagcc gtcgcctttt tgtgacgcgg catttctgct gacggacccg cagctgcgcc
     4141 cgggccgcca tgcgtaccac cacgcgggcg tgttctatgt gcaggagccg tcggccagcg
     4201 tgccggcggg tttgctgggc gtgcgcccgg gcgaacgtgt gctggacctg tgcgcggcgc
     4261 ccggcggcaa aacaagccag ctggccgccg ctctgcgcgg ggagggcctg ctggtggcca
     4321 atgaatatgt ggcggcgcgg gccggcgtgc tgaaaagcaa tctggagcgt atgggtgtaa
     4381 caaacgccct tgtgctgaat gagagcaccg cgcgtgtggc ggcggcattt ccggcatttt
     4441 ttgataaggt tctggtggac gcgccctgtt ccggcgaggg gatgttccgc aaggagccgg
     4501 aggctctgcg ccagcattcg gcggcgctgg tggcccaatg cgcggcgctg ggcgcgtcca
     4561 ttctggacga cgcggccgca gcgctgcggc ccggcggcct gctgtgttat tccacctgta
     4621 ccttcgcgcc cgaggaagac gaggcccagg tgggcgcttt tctggcgcgg catccggagt
     4681 tcacggtgct gcccgcgccg acgaacgcgg gcgcgcccgg ggaggcggcc cgatgcggcg
     4741 cgcacccctt cgcggcagag catacccggc gcatttaccc ctgccacggc ggtgagggcc
     4801 atttcatggc gctgctgcaa aagcgcgggg acggcgcgcc gccggaggcg gagcgggccc
     4861 ggccgcgcgc ggcccgcggc cagcgcggcg caaagcgcgg ccgagacatg cgcgcctgcc
     4921 gcccggtgca gggcggaaac ggcgtttccc gggcggaggc aagagccgag ggagaggctt
     4981 ttctgtgcga gtatttcccc ggcgccgcac agcttccgct ggagtgccgc ggaaccgagc
     5041 tgttcgtgct cccccgggaa ggatgcggcc cggcggaagg cctgcgcgtg gtgcaggcgg
     5101 gcgtatgcgc gggcagcgcg gcacggggac gttttgtgcc ggcgcatcac ctgtttatgg
     5161 catacggcgc gcaatgcgcc aatgccgaac gcctgacgct ggcggacccg cgcacggccg
     5221 cgtggcttcg cggcgaggcg atcccggcgg aaaccgccgc gcccggctgg gcggcggtgc
     5281 tggcggacgg cttcccgctg ggattcggca agcagagcgg gggcgtggtg aaaaaccact
     5341 accccaaggg cctgcggaat ctgaagtaaa ccggccgcat gctccccgca cccggctgca
     5401 ggagaagggt gaaatgccgg aaaatcccgt cgccaggccg aaaatcttgc gccggtgcgg
     5461 gatgtgtgct ataatctatt agttagactg caacttcaat actgaacata ttttttaggg
     5521 ggttcctttt atgtcagggc acagcaaatg gaataatatc aagcggaaaa aggaaaagac
     5581 ggacggccag cgcgccaaga tcttcacaaa gatcggccgt gagatgtccg tgtgcgtaaa
     5641 ggcgggcggc ggcgacccca acgtcaatac caagctgcgg gacctcattg ccaaggccaa
     5701 gagcatgaac gtccccaacg acaacatcca gcgcatcatc aaaaaggccg agggcggcga
     5761 aaaggacgat tacgagagca tcacctacga gggctacggt ccctccggcg tggccatgat
     5821 ggtggagacg ctcaccgaca accgcaaccg cacggcggcc aatctgcgcc actatttcga
     5881 caaattcggc ggcaacctgg gcaacacggg ctgcgtgggc tttttgttca gccagcaggg
     5941 cgttatcgtt ctggacgtgg agggcaagga cgaggagacc gtgatggagg atgtgttcga
     6001 cgcgggcgcc gaggacttcg acatgggcga cggcgtggcg gaggtgacca ctaccccggc
     6061 cgcgttcagc gacgtgcgcg ccgcgctgga ggacaagggc tatgaattca tttcggcgga
     6121 cgtggccatg gtgccctcca cctacaccgc cctgtccgac ccggatgacc tgaaaaaaat
     6181 gggcatcctt ctggaccatc ttgaggatga cgacgacgtg cagaacgtgt ggcataacct
     6241 cgaaaacgaa gaggaccttg accgctgatg gcgcgcgaca tgagccggaa cagaaagggg
     6301 atataagccg tgggcgaaca gatcgtttgc agcaactgcc tgtccaacgc ggattcgggc
     6361 ctgcccgcgt gcccgtactg cgggcagagc tttgagaata ccaatcccgc cggcacgctg
     6421 cccgtcaaca cgctgctggc cgggcgctat accatcggcc gtgtgctggc gctggacggt
     6481 gagggcgttt tgtacgctgc catagacaac agcgccacgc gccgcgtggt catcaaggaa
     6541 tatgtgcccg tcaccatctg cgccgcgcgt tcccgcgacg gcagcgtcat accgcgccag
     6601 ggccgcgagg tgctgtttaa gacgacgcgc atggattttg tggacctgta ccgcagcctt
     6661 gtcgcgctgg ggcgcaacga cggcctggtg caggtgctgg acctgattga ggaaaacaat
     6721 accgcttacg ctgtgcgcga gccggacgag ggcacgccgc tgctcactta tctggagcag
     6781 cgggcccagc cactgacgca gagcgaggcg cttctgttgc tgcgcccggt ggtctatggc
     6841 gtggaggcca tgcaccgcat gggcctgctg caccggggca tcagccccga gacggtgttc
     6901 atcaccaaaa cgggctcggc caagctttcg ggctatgcca cgctgggcct gcgcaccgcg
     6961 gacagcgagc tgaagagcca gatgttcgac ggttatgctg cgccggagca gtacgccgtg
     7021 gcggaatttg acggaaaata caccgatatc tatggcttgg gcgcgctgtt ttaccgcgtg
     7081 ctcacgggca agacgcccgt gccggcaaac ctgcgccgga tgaacgatac cctgccgccc
     7141 gcccatacgg tggataagga gatcccgggc tttgtctcgg cggccatcgc gcgggccatg
     7201 cgccttgctc ccggcgagcg catgcagtct gcgtccgacc ttcttgcggc gatcactgcg
     7261 ccggaaaagg acgagggcgg cttcaggctg acgcagcgtc aaatcaaatt tctcgcgctg
     7321 ggcgcggcgg ggcttgtgct ggtggcggcg gtgtccatct gggccatcgt gtccgcctcc
     7381 ggtggtggag aagcatcttc atcgtcgtcg tcctcctcgt cctccggcac ctccgtgagt
     7441 tccggcagtt ctgaagtgga ggtctttacg gtccccagct tcgttggtaa aaaatataca
     7501 gacctggctt cgaaccaggc ctatacgaaa tacgtcagtt ttcttacgga gcacgagttc
     7561 agcaacgact ataaagcggg cgaggtcatg cgccagagcc atccccaggg cacgcaggta
     7621 gcggtgggca ccgtgatcac gctgacggtg agcgacggcc cggaaacggc cgtgatgccc
     7681 gaggtgctgg gccgtccgca ggcggatgtg gaggcggatc tgaaggcgct gaagatcaaa
     7741 tacgcgctgt atccgctggt gaacgacgga agctatgtcg cggggaccgt cgcgcgcagc
     7801 gacacggaca agggcaccgt ggtggagatc ggaaagacca cggtgacgct gtacatcgcg
     7861 ggcgaagcac ccaagcagcc ggacagcagc tcgtcctctc agacggcgcc ccctgccgga
     7921 gacagcgagg tcacgccgcc tccggtatcg tccgacacca cggcgccgtc cggcgacgcg
     7981 ggcggagaaa caggcggcgg gggcacgccc gaaggctgac gccgccccac gctcagataa
     8041 aatgcgaaat gcagctcata caagcagcgc cccggaacca gaggttccgg ggcgctgctg
     8101 cgtgttacgg ggcggcctgc gcgctgccgt gcaggtcgta ataaaacata tactgctgca
     8161 aaatgcccgc acagcccgcg tagcggcgga aggggaagcc cttgggataa tgcgcggaca
     8221 ggatctgttt tacatgggtg tcgaccggaa atgcatccac atgatgcaga ccgaacaggc
     8281 agatacagtc cgctaccttg ggtccaaccc cctttacggc gcgcagcgcc gtgtgcgctt
     8341 ccggatagga catggcgcgc agtgcatcca gcgcgcccgc ctgtgtaaag tgcgcgcctg
     8401 cggcacacag gtattccgcg cggtagccca ggccaagggc gcgcaacgtt tccggctgcg
     8461 ccgccgccag tgcagcgggc gtggggaagg cgccgccctg catggcgcac aggtccctta
     8521 ggtttttgcg gatgcgcggg atgttgttgt tctggctgac gatgaagctg acgatgacct
     8581 cccacaagtc ctgccgcagg atgcgcatgc cccagccata ggctacggcg gcctgaagat
     8641 atgcgtcccg gggcgccacg cgtgccttta tggcggcgta atcggtgtcc agatcaaaat
     8701 agctgcgcca cagggcgtcg aattccccgg cgccgcagga aaaatcgaaa acgccgtctg
     8761 tctgccgtac agtgagcgtg cgcccgaaag ccgggatgcg gtatgtatcc tgccccagcg
     8821 gttcccagcg gaagcactgg ccggactcgt agatgtggcg gaggtcgagg tcgggtatgg
     8881 aaagctgcat gggatgctcc tttgcttttt tgaaaacggc cgcacacacc ggcacggcgc
     8941 tgtgaccagt atatcataca cctgcgcccg tgggaagccg gggcatttcc agtcctttgg
     9001 aaatgcggcc gaaaaaattt ttcccgcagt ggaaatatat tccggaaaaa gcttgacaac
     9061 acaaactgta atgtgtatga ttacagatag aaactgtaac acaaaaaagt tcagttccgg
     9121 gacgcgcggc agaggccgtc cttttgcaaa agggcggggt atcgctgccg ggggcgcata
     9181 tactatttac aaagcacggc gcaaagcagc gctgtgccca atcaacaaag gaggcaattg
     9241 cacatgtctg cattcaatgt gacaaaagaa aatttcaaaa ccgaagtatt ggaggcaaaa
     9301 acgcccgttc tggtggactt ttgggcgccg tggtgcggct actgccgccg cattggcccg
     9361 gcggtggacg ctttggccgc agagtacgcg ggtaaggttg ccgtgggcaa ggtgaacgtg
     9421 gacgaacagc ccgagctggc cgagcagttc aaggtgatga ccatcccctc gctgttcgtg
     9481 ttccaaaacg gcgagcatgg cgaagccatc atcgcaccgg gctccaaggc gcagatcgag
     9541 gaattcatgc gggcgcaggg cgcgctgtaa cgggataaaa ggcgcgcggg agcagcgcgg
     9601 gaaaacggag ggacgataaa atggaacaga tctacgacgt ggcggtgttg ggcggcggcc
     9661 ccggcggcta tacagcggcg ctgtattgcg cgcgggcggg gctttcaacc gtggtgctgg
     9721 aaaagctttc gcccggcggc cagatggcga ccaccagcaa cgtggaaaac tatcccggct
     9781 ttgccgaggg cgtggacggc ttcgatctgg ccatgcagat gaaagacggc gcggagcggt
     9841 tcggcgtgga aacgctgttt gcggacgcaa cggctgtggc gctggaaggc ggcgtgaaaa
     9901 ccatcaccac ctcctccggc gagcgccggg cgcgcaccgt cattctggcg accggagcag
     9961 acccgcgtcc tctgggcgtg cctcatgagg atgagctgcg cgggcgcggc gtggcgtact
    10021 gtgccacctg cgacggcatg ctgtaccgcg gcaagacggt tgttgtggtg ggcggaggca
    10081 actcggcggc ggaggatgca ctgttcctgt cccgcgtgtg caaaaaggtg tatctggtgc
    10141 accggggcgc cgtgctcacc gcctccaaaa gctattggac ggcgctgcaa aaggctgaaa
    10201 acgtggaatt catctggaac tcccgtgtgg tggagctgct gtacggctcc gtggtgacgg
    10261 gcgttgtgct ggaaggtgtg gagagcggca accgttcgga actggcctgc gacggcgtgt
    10321 tcatcgcggt gggacgcatt ccaaacaccg ggctgtttca cggcgtgctg gagatggacg
    10381 cggcgggata tcttatcgcg gacgagacca cgcgcacggc gctgccgggc gtgttcgcgg
    10441 tgggcgacgt gcgcacgaag cccctgcggc agatcatcac agcggcggcg gacggcgctg
    10501 tggcctccaa atatgcagaa gcctatctgg ccgaacagga ggactgaaga aaaccaaaac
    10561 aggcgcggcc acaaaaatga acagcacccc atttgttaga cagtatggta aactagtaac
    10621 gaatggggtg ttgttttatg ccaaaagggg ttcctaaata cgatggagca tttaagaaac
    10681 agatagtaga ggaaatgcga gagaaaggat taagctaccg agaaattgca cgacagtagg
    10741 aaatcagggg agctcactct gtagcggatt gggaaaggat atatcttgaa gaaggtcctg
    10801 atgggctgtt tattgagggc cgcggcaggg gaagcaaagg atggccaccg aagaagctaa
    10861 agccggaggt agaagaagac cttctggcgg aagtacagcg gttgagagct gagaacgcat
    10921 acctaaaaga attgaatgcc ttggttgccg agagagtacg gcaagagaaa aagcaaaagt
    10981 aatatgggaa ctgaggtatg aacacaaact gtcgctgttg ctggacgtcg ccggtctccc
    11041 tcgcagcaca tactattact acaccagaaa gcagaaggac ccggacaaat acgcggagtt
    11101 aaaagaggaa atctgctcta ttgtgactga acacaagggc aggtacggtt accgcagagt
    11161 aacgcaggaa ctgcaaaacc gaggttttca tcacaaccac aagcttgtga tgaaactgat
    11221 gaagaagctg gggttaacaa gcaaggtgcg gatgaagaag taccgttcct acaagggcga
    11281 agttggaaag attgctccta atctcttgga tagggacttc tatgctgagc agtcagacca
    11341 gaaatgggtc acagacgtga cagagttctc cttgttcggc gagaagctgt acctgtctcc
    11401 aattctcgat ctgtgcagcg aggatcttat aagctgcacg atctcggagc atccgatgct
    11461 cagtatggtc acttccatgt tggagaaagc ttttgacaca atcccagacg gtacgggact
    11521 gattcttcat tccgatcaag gctggcaata ccagcacaag cattaccaaa agatgttgga
    11581 agacaagggc gtccggcaga gcatgagccg gaaaggcaac tgcttggaca atgccgtgat
    11641 ggagaacttc ttcggcttgc tcaaaacaga actgctttac cttcaagatt tcgactccct
    11701 ggaacacttc aaagccgagc tcattgacta tctctactac tacaataacc gcagaatcaa
    11761 gctaaagcta aagggcctga cacctgctca acacagatac cagacccttc aggtcgctta
    11821 attacatatt ttctctaact tttggggggc acttcaaaa
//
LOCUS       DAWBSB010000046        11798 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1290_length_11798_cov_2.447245, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000046 DAWBSB010000000
VERSION     DAWBSB010000046.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 11798)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11798)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11798
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1290_length_11798_cov_2.447245"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    8019..8028
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2515 a         3195 c         3743 g         2335 t
ORIGIN      
        1 agcgggcaag ggccaaccgc cgcacgctgc tcatcggcgc gggcgacgca gccagcacac
       61 tgctgcacga gtttgcaaaa aacaaatccc ctgaaatgaa catcatctgc tgcgtggacg
      121 acgccccgga aaaggtgggg cgttccatca tgggcattga gatcatggga actacggagg
      181 atatcccgga gcttgtggag cgctgcgaga tcgagaccat cctgttcgcc atcccaacgg
      241 tggatgacga aaacaagcgg cgcatcctga gcatctgcaa caagacaaag tgcaacgtgc
      301 gcatcctgcc ggatatcgtg cagctcatcg ccaacggcgg gaaggacgtg ctttcgcgcg
      361 tgcgggacgt gcgtgtggag gacgtgctgg gacgtgagca gattgaatta acagacctga
      421 cgaacaccct tgtctccggt aaggtcgtga tggtgacggg cggcggcggt tccatcggtt
      481 cggagctgtg ccgccagatc gcggcctgcg ggccaaagcg gctcatcatt gtggatattt
      541 atgaaaacag cgcctattcc gtccagcagg agctgaagcg ccgctacggc agtgcgctca
      601 agctggatgt atgcatcgcc tctgtgcgcg attccaaaaa ggtggaccgg ctgttcgcgc
      661 gctatcagcc ggacgtggtg ttccatgcag cggcgcataa acatgtgccg ttgatggagg
      721 acgcgccgga ggaggccgtc aaaaacaacg tgttcggcac ctacaatgtg gcgctttcgg
      781 cggacaaata cggtgtgggg cgtttggttc tcatctcgac ggacaaggcg gtgaatccca
      841 ccaacgtgat gggtgcgaca aaacgcctgt gcgagatgat cgtccaggcg ctggcgcaga
      901 aaagcaaaac caaatttgta gcggtgcgct tcggcaatgt gcttggttcc aacggctcgg
      961 ttctgccgct gttcaaagag cagatcgcgg cgggcgggcc tgtcaccgtt acacacccgg
     1021 acatcgtgcg ctattttatg acgatccccg aggcggtgcg tcttgttctg gaagccgggg
     1081 ccatgggcaa cggcggagat attttcgtgc tggacatggg caaccctgtc aaaattctgg
     1141 atctggctga aaacctcatc aagctgtcgg gcttcatccc gtaccgcgat atcgagatca
     1201 agttcacagg ccttcgcccc ggcgaaaagc tgtatgagga gctgctgatg gatgaggaag
     1261 gcctgcgcca gacagataat aagaagattt ttgtcggtgc gccgctcaag ctcaataaag
     1321 ataccttttt cgaccatctg gccacgctta agcagatcgc ttacagcaac aacagcgaca
     1381 acctggtgca ggcgctgatc gatctggtgc cgacgttcca tcatgcggaa aataactacg
     1441 actaaggagc gcagccgatg tataaaagcg ttgtgaaacg tgtgttggat tttttgctgg
     1501 cactggcggg caccatcgtg ctttcgccgc tgctgttggt gttggcggcg tgggtcaagc
     1561 tggattccaa gggccctgtc tttttccgcc agaaaagggt gggccgcgac aagcgtctgt
     1621 tcagcattct taaatttcgt tccatgtatg ccgatacgcc tcatgacgtg cccacgcatc
     1681 tgctgcagaa cccggaggcg atgatcacgc gcagcgggcg ctttttgcgg cgaaccagtt
     1741 tggatgaact gccgcagctg ttcaatatcc tggccgggca gatgagcgtc gtcgggccgc
     1801 gtccggcgct ttggaatcag gcagacctga ttgcagagcg ggataaatac ggggcaaacg
     1861 cgctggtgcc cggcctgaca ggttacgcgc aggtgaacgg ccgggacgaa ctgcccattc
     1921 ccgtcaaggc ggaactggac ggttattatg cggcacatgt aagctttgtg ctggatatga
     1981 agattttttt caagaccatt ttcaatgtgc ttgcgggccg cggcgtggta gaaggcggta
     2041 cgcagggcaa ataagcttga cgcgcggccc gcggtgtgat aatctggtag aaacagccgc
     2101 agaagcgatg gcctgcgctt ccgttccttt atggctttgc gcagcgcagc ggcggtaaat
     2161 tgaatcagca agatggcgat gacaaggaaa cagtaagcgc ggctggatgg cgcagagaga
     2221 ggatggctgg tgagaatcct cccggaaggc ccgcggaacc cctccttgag ccgcgcagcg
     2281 aacgggcctg tcctcattaa gctgtgccgg tcccccaccg ttacccgggg aacgcatccg
     2341 gcgttttgcg ccgtgtgcgg cggagcgcgg gatatttccc gaactcaggt ggtaccacgg
     2401 acattctgtt cgccctgagc cgactgttaa ccagccggcc cggggcgttt tttgcagtat
     2461 gcgggaaccg tccggcccgg ccgaacacaa aggaggagcc gaatatgaag ctggacaaat
     2521 tggtggggga acgttttcgg gaacggcctg cgggctgtgt catcgaaagc cacgcgctga
     2581 tggtgcgcgg cggttacatg aaaggcgtcg caaacggtat cttttcccag tatccgccct
     2641 tgcgccgcgt cacccgcaag ctggagcaga tcatccgcga agagatggat gcgattgaag
     2701 gacaggaggt gtccatgcct gtggtgctgc ccggcgcgct gtgggaggaa tcgggccgat
     2761 atgtgagcgt gggaagcgag ctgctgcgtt tccaagaccg cacaggcact cccatggtgt
     2821 tgggcatgac acacgaggaa gccgctgtgc agctcgtgcg ggaatacggc cggacggctg
     2881 cgcggtatcc ctttatgatc tatcagatcc agaccaagtt ccgcgatgag gcccgtcccc
     2941 gcggcggcct catccgtgtg cgggaattca cgatgaagga cgcatattct tttcatactt
     3001 cccagcagga cctggaacgt tattacgcgc gttgccaccg cgcctacgag cgtatttttg
     3061 cccgggcagg cctgccggag gtggtttccg ttgcatcgga ttccggcatg atgggcggca
     3121 gcctttccca tgaattcatg ctgttgacgc ccgccgggga ggattctgtg gcggtgtgcg
     3181 gcgcctgcgg ctaccgtgcc aatgtggagg ctgcggcctg tgtggcgcac aatgagccgc
     3241 ccgcagtttt gcagccgctg cagacggtgg aaacgccgga cgcgcacacc attgaggcgt
     3301 tgtgcgcctt tttgcatgtc atacccgcgc agtgctgcaa ggctgtggta taccaaaagg
     3361 agacggatgg cagctatgtg gtgttgtttt tgcggggcga cctggaggcg aaccaggcca
     3421 agctgaccaa ttatctgggc tgcgatgtcc ggccggcggc tattacgccg gaaagtggcc
     3481 tttgcgcggg ctttatcggt ccggtggggc tgtgcggcgg gatgacggtg ctgtatgaca
     3541 catccctgca gggcacgggc aatctcgtct gcggggcaaa ccggcaggga ttccacagta
     3601 cgggcctgca gcttgaacgg gactgcggaa cggtggaata tcacgatttt gccaagctgc
     3661 cggacggcgg tgtgtgtccg gtgtgcggca ggcctgccat tcgggtgtct cgcggcgtag
     3721 aggtgggcaa tatctttcag ctgggcgcca agtatacaca ggccatgggc atgcgttatg
     3781 ttgatgcgga cggtacggaa aaaacgcctg taatggggtg ctacggcatc ggcgtgggcc
     3841 gcttggccgc atgcgtgtgc gaggtccggc acgacgacca cggccccgtc tggccgctgg
     3901 ccatcgcacc ctggcaggtg catctgtgct gcctgcgggc ggatgacgcc ggaacaaaag
     3961 cggcggcgaa tgcattgtac gcatcgctgc agaaaaacgg tgtagaggtg ctgtacgatg
     4021 accgcaccgt gagcgccggc gtgatgtttg cagacgcgga cctgctgggc atacccgtgc
     4081 gcgtgatcgt cagcccgcgc aactgcgcag aaggctgttt tgaactcgcc gcaagggata
     4141 agacggtaaa ggagaaagca ccccttgcaa aagccgtgca ggctgtgcag gcgctgctgc
     4201 gccgccttga ggacgaggcg gccgcacagg tgccgcccag gctttaaaac agcggctcgg
     4261 actgtggaaa taaagaacag ccgaaaaaac aaaacccagc ggcacgtttc cggattcgga
     4321 agcgtgccgc cgggtttacc aggatcatca gagcgggcgt tcaccgccct gacggctgta
     4381 aaaccagtac gtcgcagctt tcagaaggat ggcggcggcc agcacggaaa atgccgtgta
     4441 aaaagccgcc atgtcgaaca tcgacgcggt gagcgtgaca aagagcagga tggcgagcag
     4501 gatatccgcc acaatgccgc cgcttttgtc gtgcagatac tggttgcgtt cgtccagttc
     4561 cgttttcatc tgctgctgca gaagctgcct gcttcgcagc agctgtttgt ttttgcaaat
     4621 acgtccgatg acatagccca gcccgccgaa gaacacgatg cggctgaatt tgtcggcaag
     4681 cggcgtcatc atgcgcgagt ctccgccgcc aagctccgcg ataagcacca tgtacacgag
     4741 cattccggta agcagcagcc aaagagcgcg gatgcgccat tgaattttcc gggtataggt
     4801 caaatgttta caggttttca tacacagcgt cctccatgct cttgttttcc gcaagacagc
     4861 acaattcctc cacggtcacg ccgaacacca gtgccagccg gtatgccaac atcagcgagg
     4921 ggctgtattg ttctttttcg atggagatga tcgtccggct ggaaacatgc gtccggtcgg
     4981 caagctgctg ctgcgtaagc cctgcggcgg tgcgcagctc tttcagtttt gttttcatgc
     5041 gttcctccgg atgtgaagct tacttcttat gaagtcgact tcatattaac aggccggaac
     5101 ggttttgtca ataggggaag gaggaacagg aggaaaagct gcgggcggct tgggaatata
     5161 gggctgtcaa aggacatgct tttgttccgc ggcaaaaacc ggcgggattt ttcccgccgg
     5221 tgtggttttg cctgtgccgc acggctcaaa gattcgtgct ctttttcaga agttccgcaa
     5281 ccagagcgtc gttgctcgtc agcgcagcca gcatcagctt atagatgccg tctccgtttt
     5341 caataaactg cgcgcgggcc agtttggccg tcagcttatc cggaacgtaa agggagaagg
     5401 agaacacgtc gctctccata tcgcggatgc tgcattgcac attgtaaccc gttccggagg
     5461 ccgcgggcgt gatctgagcg tgatgctggg cctctttgcg tgcaaactgc tgcgcggcca
     5521 gagtggcgcg cacggccgcg tcccgtacgc tgcggggcac gtcgtcggcc agcgcttcag
     5581 cgatgtccgc gcctgcggga agaatagtat aacgcccgtt ttcctcgcgc acgaagccct
     5641 gttccagcag atccgaaaga ttgctggcaa gctcaaaata attcacaagc tcttcgccaa
     5701 gcagcgcggt ctccagctct ccctgggaga ggggcgtgcc cgcgtgctga atcagatagc
     5761 acagcaggat gcgtatttcg gtggatgtag tgagcccgcc cggctttacg ccggcggtaa
     5821 aagcgtttcc cgccatggtg tccctcccgg tgatatcctt tgtgcaagga tgcgatactt
     5881 tattttaatc ataccgcgcc ggggccgaaa agtaaagcga aagacggcgg ataaggcgag
     5941 gaaaaacaac gccgcggcag tgagacagca ctgcgcggca aggctttcag ccatcaaata
     6001 tcgtttctat cacgcgggcc gcgcccggca cggagtatat ccaccgcccg atatgtccgg
     6061 cgtgcaggcg gtattccagc ggaaaggcga aggtctcgtc ccgcatagca tgcagcacca
     6121 ccagcttgat tttcattttt tcgggaataa tgcttttgat atatacgctc accagttggc
     6181 cgcagtcaag ccccggagcg gcctcggcca gccccgcgag atttggagcg agctctacga
     6241 acacaccgta gggctccacg ctgcgcacca ggccgaccac tgtttcaccg gcggcaaagg
     6301 cggcggcgtt ttccagccag gtgcccagca gctctttcat cgtcagcaca atacgccctt
     6361 cggtgtcccg gctttttacg gcacacagga tatcctggcc ttcgtgcaca cggtcggccg
     6421 ggctttgaat acgggacacg gacatacagt cgatgggaag aagagccggg accccgcagc
     6481 cgatgtcgca aaaggcaccg aagctctcca gatgcgtcac ccggcagggc aggatgtcgc
     6541 cgggcagaag caggtcgaga tactcggctt tacagcgctg ctgcgcctgt gtgcgtgaga
     6601 gcagcgccac aggcgcggcc ccctgttcgt cgatctccat gaccgtaaag cacgcgggcc
     6661 ttcccacgcg tgtgatcagc gcgatgtcgc gcaccgcgcc ttcgcgcaca ccgtcggcgc
     6721 attcctcgta gggcattatg gcttcgatct cgcccagccg gaaatgcaga cgcttttcgc
     6781 tgtcgaagga gagcgcgcgg ctctccagga tgtcgcccgc agccatggcg cggcgcaggg
     6841 tgtccagagt gtagcttgtg gggaagcgct gtgctccctc agcacgttat tccatgtttc
     6901 caaccgtcct tcatgcttcc attcgcgcgc cgggcaaagg gccgtcccgc gggcagagtg
     6961 tttgggacgg ccggaaaggg gcgcactggt aaaatatatg tgaacacaag cgggaatatt
     7021 tgctaaacgg gacggtttga tatataatat ttaagcactc ggcattcccc aaaaatgggc
     7081 ggccggcgca tggaagttat cttgacggga gaaaacagca tggacgttca ggtgggcgac
     7141 agcatcatca caaaaaaacc gcatccctgc ggcaacagca ctttcgctgt gctgcgcgtg
     7201 ggaatggatt tccgtatccg ctgtaccaaa tgcggacggg aggtcatggt gccgcgcagc
     7261 aaaattgaaa aaaacatcaa aaaagtggtg cgcgaggctt tgccgctcta gccgcgcttt
     7321 cctttgccgt acaaaagcag ttttgaaaca gcgataagag gagagcgttt ttattatgga
     7381 tatgattaca cagctcaggg ctgtgcgcaa ccgttatgac gagatcaaca atcaattgca
     7441 ggaccccacc gtcatcaaca acaacgagct gtaccgcagc ctgatgaaag actataaaaa
     7501 cctgacgccc atcgtggagg cctacgacgc ttatgatgcg gcaaaaggcg cctttgacga
     7561 agcaaaggaa atgttggacg aaggtggact ggacgctgaa ttcaaagagc tcgcgcaggc
     7621 tgaatatgaa gaaaacaagg aaaaaatggc gtttctggag gaacggctta agatcctgct
     7681 gctgcccaag gatgaaaatg atgaccggag cgtcattgtg gagatccgag ggggcgcggg
     7741 cggcgaggaa gccgccctgt tttcgcacag tctgttccgc atgtatacga tgtatgccga
     7801 aacgcggggc tggaaatatg aggtgctgaa cgccaacgag acagagctgg gcggtatgaa
     7861 ggaagtgagc ttctccatcg agggagaggg ggcgtacagc cgccttaaat ttgaaagcgg
     7921 cgtacaccgc gtacagcgcg tgccggagac ggagacacag ggccgcgtac acacttctac
     7981 cgtaactgta gccgtaatgc ccgaagccga ggaggtcgnn nnnnnnnngt cgagatcgac
     8041 atcaacccgg ctgacctgaa gatcgacacg tttcgttcct cgggcgcggg cggccagcac
     8101 atcaataaaa cttcatcagc gatccgcgtc acgcatctgc ccacgggggt ggtcgtggaa
     8161 tgtcaggacg agcgaagcca gtacaagaat aaggacaagg cgctcaaggt actgcgcagc
     8221 cgtctgctgg cgcaaaaaca ggccgagcac gattctaaaa ttgcggcgga acgccgcagc
     8281 caggtgggca cgggagaccg ttccgagcgt atccgcacct ataactatcc tcagggccgt
     8341 ttgacagacc accgcatcgg ccttacgctc tacaagctgg atacgatcct gaacggcgtg
     8401 cttgatgagg tcgtcgacgc gttggtaacg gccgaccagg ctgaaaggct gaaggcttcc
     8461 acggaggaat gaattttgca gacctgtttg aaacaagccg gcgcggcgtc tgtcgctgag
     8521 gccgcacgcc ttttgcgggc gggcgaggtg gtgggcattc ccaccgagac cgtgtacggc
     8581 cttgcggcca acgccctttc ggacgaggcg gtgccgaaaa tatttgcggc caaaggccgt
     8641 ccacaggata atcctctcat ttcccatatc gcatctctgg acatgctgcc tatggtggtg
     8701 cgcgaagtgc cgggcgccgc ttaccggctg gcggaggcgt tttggcccgg cccgctgaca
     8761 ctcatactgc cccgcagcaa aacggtggcg gactccgtgt gcgcagggct ggatacggcg
     8821 tccgtgcgta tgcccagcca tccggtggcg ctggcggtca tccgcgcagc gggcgtgccg
     8881 ctggcggccc cctcggcaaa cctttccggc agcccaagcc ccacaacagc ggcagacgtg
     8941 ctggcggata tggagggcaa gatccctttg atcctggacg gcggcgcgtg cagcgtgggg
     9001 gtggagtcta cggtgatctc tctggcagga gcacagccgg tgctgctgcg tccgggttat
     9061 atcaccaaag agcagctgga agatgcaatg ggctgtactg tggcgctttc ggacgcagtg
     9121 ctgcacaaat tgaaagacgg ggaaagcgcg gcttctcccg gcatgaagta taagcactac
     9181 gcgcccaagg cggacgtaac catcctaaaa ggcagctttg atgcatataa agaatatatg
     9241 aaatcgcact gcgcggatgg tgtatatgca ttgtgcttta caggggagga accggcgttg
     9301 ccgtgcccct gcgtgacata cgggtgcgca gacaagccgg acgagcaggc gcacgcgctg
     9361 ttttcagccc tgcgtgaact ggatgcacgc ggtgcaaaaa cggtgtttgc ccgttgcccg
     9421 gcgcaggagg gcgtggctat ggctgtctac aaccgtttgc tgcgcgcggc ggcgttccgt
     9481 gtggtggagg tgtaggctgt gaaggtgatt gccattacag ggcgctccgg ctccggaaaa
     9541 agcacggttt cggcctatta tgggtctttg ggatatcctg tgctggatgc ggaccggaca
     9601 gcacgtgagg tgacgcgtcc cggcgggcct tgccttgcac agttgtgcga tgcattcggt
     9661 tcggacatcc ttctgccaga cggcacgctg gaccggggct gcctggcggc ccgggccttt
     9721 gccacaccgg agggcacgcg gcggctgacg gatatcacac atcccgccat tatccgggag
     9781 ttgctggacg gcgtggctgc ggcacaaagc acgggcgttc cgttcgtgtt tgtggacggt
     9841 gcggtcatcg tgggagaggc attcgagcaa tactgcgatg cgatcatcgt cgttaccgcg
     9901 tccgagcggg aagctgtttc ccgcatcgtg ctgcgggaca gcatttccaa gcaggcggcg
     9961 cgtatgcgcc ttgcggcaca gacgccggag gacgtactgc gtgctgcggc agattatatc
    10021 atagcgaata cgggagacgg gcggcatctg cgcgcggcgg cgcaggcggt gctggaacgt
    10081 ctgctcgcaa aggagacgaa tggaacaaaa gaaaaaacat aaccggaata tctatatcgt
    10141 gctggctgtc gtgctgtgcc tggcgggtgt ttttttcgta aaggacgcgg tgcgcgccgt
    10201 ggaggaaatg gcatatccgc gcacctatgc cgcgctggtg cagcagtatg ccgcagagta
    10261 cggagtggac gagaataagg tgtatgccat catccgtact gaaagcggct tcaagccgga
    10321 ggcggaatcc aaggtggggg cacgggggct gatgcagatc acagacgaga cgttcctgtg
    10381 gatcaaatcc aagatcgcgc ccggcgagga gattactttc gacgatctgt tcgacccggc
    10441 ggtgaacatc cgcttcggca catatctgct tgcgacctgc cttgcacgct atgacgggga
    10501 tttttccact gcggcggcgg cgtaccacag cggcatgggg cttgtggaca gtctgctgca
    10561 gaaggcggaa tattctgcgg atggaaaaac tcttacggca ttcccctatg agcagatgaa
    10621 cctgtacgtg cataaggtca ataaaaatta tcaaaaatat ctcgcgcttt atgcgcagga
    10681 tgaaggagtg taaacaatgg aagaaaaaaa gagcctgggc aaggaattgc aggaaaaact
    10741 gacgttcaca cagccgcata ttgcagatga acagccggac gcggttgcgg aggccgagcg
    10801 cttctgtgtg gactacaagg cgtttctgga cgcggccaag accgagcggg aggcggctgc
    10861 cgaggtggaa cgccgtgcgc tggcggcggg ctatcggccg atgcagcgca cgggcgttac
    10921 atataaagcc ggtgacaagg tgttttacaa caaccgcggc aaggccatgc ttctggctac
    10981 gctgggcaag cgcccgctgg acgagggcgt gcgtttgatg gtagcgcaca ttgattcccc
    11041 gcgtcttgac ctcaaaccga atcctttgta cgaggacagc gacatcgcgc tgttcaagac
    11101 gcattattac ggtgggattc gcaaatacca gtggggagcc acgccgctgt ccctgcacgg
    11161 tgtagtgtac cgcaaggacg gcaccaaggt ggaagtccgc ctgggcgagg aagcgggaga
    11221 ccctgtgttt tgcgtgacgg acctgctgcc tcacctgggc gcagagcaga ataaacgcac
    11281 gctggcggac ggtctgcggg gcgaggagct gaatatcgtg gtcggctcgc tgccttatgc
    11341 ggacaaagag ctgaaagaac gcgtcaagct gtatacgctg acgctcctga atgagaaata
    11401 cggcatcacc gaacgtgatt ttacccgcgc ggagctggag gccgttccgg ccgtcaaggc
    11461 gtcggatgtg ggctttgacc gcagtatgat cggcgcgtac ggccacgatg accgtgtgtg
    11521 cgcctatacc gcgctgcagg cggagctggg ggtgaaggaa ccggaataca ctacggtatg
    11581 cattctggca gacaaggaag aagtgggctc cgacggcccc accggccttg tgagcgattt
    11641 tgtgttccat ttcctgggtt atcttgcgga cgcacagggc gcaaactata aactgatgct
    11701 ccaaaatgca aaatgcctgt ctgcggatgt gaacgcggcg ttcgacccca cgttcccgga
    11761 cgtgctggag aaacgaaatg cctcgtacct gaaccgtg
//
LOCUS       DAWBSB010000047        11753 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1295_length_11753_cov_3.026928, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000047 DAWBSB010000000
VERSION     DAWBSB010000047.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 11753)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11753)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11753
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1295_length_11753_cov_3.026928"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2786..2795
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6131..6140
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2323 a         3309 c         3646 g         2455 t
ORIGIN      
        1 tagaaatatg gtataatacc ctcacgcccg ccgcttgcgc ggccacgcgc acttcgtgcg
       61 ctgttgcgct ttcgcgcatg ggctttgaga gcattgaacc atgcaaaacc gcagcaggcg
      121 cggttttgtt gaagccgttt tgcggcgtcc tgctgtaata ttagcgatcg cggctacatg
      181 ccgcaaaaca gaaaggatgg acaacgtggg tacgattttt tataccgcac ctgtggcgcc
      241 ccatgtgctg atcaacgcat tcttccttta cagctttttg ggctgggtga tggagtgcat
      301 cgtcatacgc cgggagaagg gccggtggga gaaccgcggc tttgcgcatc tgccgttctg
      361 tatcatctac ggcttcggcg caatgctcgg ctttgcgctg ctgcgtccgt tttccggcaa
      421 ttatgtgctg ctctatcttg tgggagccgt tttggcaacg ctgttcgaat acctcaccgc
      481 gcggctgatg ctgcgcctgt tcggcacctt ctggtgggat tacaccaata agccgttcaa
      541 ttataagggg attctgtgcc tggaaagcac gctgggctgg gggctcatcg ctcttctgct
      601 gtttgcgttt ctgcaccggc tggtgttccg cgctgtcctg ctcattccgg atcgtgtcgg
      661 tttggcggct gcatttctgc tggtgtgtgc ctatgcggtg gattttgccg tcagcgcgta
      721 tcaggcgtat cagaatgcgc atacttccac agaggccgag cccgtgcccg gccgcgccgc
      781 ccgctgaaaa gcgcggcgcc gacctttaca caaaggggat tttttagaaa tgatttaccg
      841 cagcaaggaa accgtgtgct ccatcacggt acccagagat ccggaatacc tggctctggt
      901 acaggatatt ctggaacatc cgctggtgcg ttccatggag gcgtacaccc agcacggcga
      961 gacctcgtgc ctgcgccaca gcatcaatgt ttcgtatctc agctacctgt attgcaggga
     1021 ccatggatgg caggcccgcg ccgccgcccg cgccgggctg ctgcacgacc tttttctgta
     1081 cgactggcac ttccacgcaa aggaaacggg caattatttt cacgggctga cacatccgcg
     1141 gcgcgcgctg gagaacgccc agcgcctgtt cagccttacg gacagggaaa agaacatcat
     1201 cctgcggcat atgtggccgc tgacgattac accgcccaaa taccgcgagg cgtatgtgat
     1261 tgtgatgttc gacaaatact gcagcctgat ggagacgttc cacaagcccg tgatggagct
     1321 gatggaatac caggagctga cggctgattt gaaaaaaggc tttgccgcgc agtgagcggc
     1381 aaagcctttt tatgtgcgcc cgggccatat tgcgcccggg ccgttttgcg gtttggatta
     1441 acggctttga gggagcggac cgcgcggcgt cgggagttgc tccgcatgcc gttttgctcc
     1501 gggcggctca caggcgctcc acgattcccg tgaccaggcg ctccagcacc tttgcgcggg
     1561 ctgcggccgt ctcgatgacc tcgttttcgt ccagcttctt ttccagcacg cccgcggcca
     1621 tattggtgac gaggctgatg gccagcacag gcagcccgca gtggctggcg gcgatgacct
     1681 ccggcaccgt ggacattccc acggcgtccg cgcccagcac gcggaacgca cggatctccg
     1741 ccggagtttc gtagctgggg cccatatagc ccagatatac tccgtggcgc agtgcaacgc
     1801 cctgcgcggc ggccacctcg tctgccagac ggcgcagagc gggcgtatag gcgtaggtca
     1861 tatcacagaa gcgcgggccg aagcccgcgt cgttggggcc ggtgagcggg ttggcgccca
     1921 taaaattgat atggtcgtca atgaccatca ggtcgcccac ctggaaatcg aggttcacgc
     1981 cgccgcaggc atttgtcacc agcagcgttt ccacacccag cgctttcatc acacggacgg
     2041 gcgcggcgat gtgggacatg ggatgcccct cataaaaatg aaaacgcccc tgcatgcaca
     2101 cgacgccgtg cccggccaga cggcccagca caaaacgtcc cgcgtgcccc ggcgcggtgg
     2161 aaacgggaaa gccgggaatg gaggcgtatt ccacaaaaga cgcatcctcc agcacatcgg
     2221 ccagtccgcc cagcctgctg accaggatca ggcccaccga cgggcgtgcg ccggccacgg
     2281 cgcggatggc atctaccgcg ttttggatca tttccttcaa ggcttttcct ccgttcgcgt
     2341 ctggctgctt ttcagctcga agtgaatcag caggctgagc agcgcgcgca ggatgatgac
     2401 cgcgcccaga atgagcagct cgctcatctc ccgtaccaga acggtcttta agatctcggc
     2461 ggccatttta aattccagcc cggtggcaag gccgtcggca agctcgaact gcagcgcgta
     2521 atgccggtgc agaaaggtat tgcccagata ttccgcaaag gcccgcactg ccgacactgc
     2581 caccacaaaa atgcccatca tctccagtac gccgatgatg ggcggcagga tgagctcgat
     2641 caaatgttcc aagtccatgt tgcgtctcct ttccaaattc ctgtctacca gtatacagga
     2701 tgtcccggca aaaaggcaat acgcgccgca catactttcc cgtccgggtc cgtatacata
     2761 aaaaagggaa aggggcgtgg acgcannnnn nnnnntggac gcatggcgcg ccaaagctat
     2821 ttgaaaaacg cggccattct gacgggaaca ggcctgctgc tgcgtgccgc aggcatgttc
     2881 ttccgcattt atatcgcggc gcgtatcggc gccgagggca tgggcctgta ccagttgatc
     2941 tacacggtct acacaatggc cgtcaccttg gctacggcgg ggctttcggt ggcggccacg
     3001 cgtctttcgg cggaactgct ggccaccgac gaccccgcca atgtgcgcgc agccatgcgc
     3061 cgcacactgg cgctggggct tgggcttggc gcggctgcgg ccgcgctgct gttcactgga
     3121 gcggggcttg cagcggactg gtggctggag gatgcgcggg ccgcgctctc gctgcgcatt
     3181 ttggcgccca gtttgccgtt tatggcggtt tcggcatgtc tgcgggggtt tttcatggcg
     3241 cggcgcaagg tggggccgaa ttcccgcgca cagatcttcg agcaggtcgt gcgcatcggc
     3301 gtggtggcgc tgctcattga ttctgccgta ccgcagggca tcggaacggc ctgcgcggcc
     3361 gtggtcgtgg gcaatacggt cagcgaggct gcgagctggg tatatatgga atggtgctac
     3421 cggcgtgagc tgagggacgt gccccgtaac gcaaagcgtc cggtgcaggg gctgggacgg
     3481 cagctgtggg atatcatagc gcccattgct gcgaaccagt atatgacgag cgtgctgcgc
     3541 accgttgaaa atgtaatggt gcccggctgc cttgcgctgt atacgctcag ccgcgaaaca
     3601 gcgctcagcc agtacggcgc gctgaaaggc atggccatgc cggtgatttt ttttccgttt
     3661 tcatttctgg ccacgctttc tacgctgctg ctgccggaga tcaccgaggc gcatgtgcag
     3721 aacaggcgcg cggcgctgga gcatctggtc aaccgtgtca tgctcatcac gctggtgctt
     3781 tcgtttctgg ccggcggcct gttcacccag tttgcaaagg agatcggcct ggtgctgtac
     3841 cagagcgagg agatcggttt ttatctgtcg atactggggc cgctgatgcc ctttatgtat
     3901 atggagagca tggtggacgg catcctgaaa gggctggggg agcagctctc ctcctttcgt
     3961 tatacggcgc tggattcggt tgtgcgcatc gcgttgatct acctgctgct gccgcgcttc
     4021 ggcatgcagg gctttttgtt cgtgatgctg gtgagcaatc tgctgacgag cctgctgaac
     4081 ctgcaccgcc tgctgcatgt tacagggctg cgtgtgcaat ggctgcgttg ggtgataaaa
     4141 ccggtgttct cgtttttgtg cgcgggcgcg gcgtgccggt tcgtgctgca gccgctgacg
     4201 cagcggctgg gcctgccgct tctggcctgg gctgttttgg gagcggtatt caccagcgtt
     4261 atttacgcgc tgttcatctg gcttacaggc tgcgccgggc cgggagactt tatcgtcagg
     4321 cgcacccgga agaaggcggg ggagagggat tcgtccgtgt gatgcggatg caggaaagga
     4381 cgccgtggct gtgtaaaatg ccgattcttg cgcgcaaagt ttggataaga tggagaaagc
     4441 tgaaaaaatg gttgacggaa gactggaaaa ggattattat gttgtcataa caacaaggcg
     4501 gccaacaaca tcctacaggc ggccttgcag cgcccggcgg cggaatgcct gccgggaggg
     4561 aaagaggtgc ctttggtgaa agcggtaccc aggtacttgc agattatcaa ttattatcag
     4621 ggaatgattg acgcgggcaa gctgtcggag gggcagcaga tgcccacgga agaggccatc
     4681 ggcgcgctgt tcagcgtgag ccgcatcacg gtgcgccagg ctttggacgg cctggcacag
     4741 gcgggatata tttacaaagt gcagggaaag ggcagctttg tcgcatccaa gaaggcggga
     4801 atgcagctca atcacctgat cggcttcagc gacgagatgc gcagtctggg gctggagccg
     4861 tccaccatcc ttgtggaaca gagcctgatg ctgcccacgg aggcagtggc aaaggcgctg
     4921 aacatcgacg tcacccagaa gatctatttc ctaacgcgca tccgctgcgc ggacggcgtc
     4981 cccatggctg tggagaaggt ccatatgccc ttttaccgtt tcgcgggcat tgaaacgcag
     5041 gatctgagca aatctttgta cgcgctgctc aaggagcaat acggctgcga atgcagcaag
     5101 gcggtgcaga gcatccaggc cggagcggcc agctcccatg atgcgaagct gctgaaaatc
     5161 aaaacaggaa tgccggtgct gtgcatctcc cgcactacgt acggtatgga tggcgcgccc
     5221 tttgagtatg tggattctat ttaccgcggg gataaatata tctttaacgt cactctggaa
     5281 aagtaaactt tcgctgggcc tgccccgcct gcggctgggc tgcgcccgcg aaaaattttt
     5341 tgaacagtat tgttataaca acatgacaac ttgtcttgtt gcagcttaaa tcaaatgagg
     5401 aagggcagag gttcatgacg gaaaaaagta gatcagagcg cggcggacgg cgcaggttca
     5461 acctgaaacg gcgcgaggcc tgcacgggct ggctgtttgt ctcaccggcg ctcatcgggt
     5521 tcagcatttt caccttcggt tctatcctgt attcgctgta cttatccttc accgattaca
     5581 acctgctcag caagccgaac tggctgggcc tgcagaacta cgtcaaggcg ttcacggacg
     5641 accagtactt ttacccgtat ttcggcaata cgttctattt tgtcgttgcg ctggtgccca
     5701 tcgtgctggt ggtatcgctg ctgctggcca tcctcatcaa caaaaaggcg ggcaggctga
     5761 caaagggcta ccgtgtagcg ctgttcctgc ccagcatcac gtccaccgtc gcggtgagca
     5821 tggtgtggct gtggatcttc aacccggaca tgggcatcct gaacaacatt ctcactgcca
     5881 tcggcctgaa tgatgtgccg ctgtggctgg gcgacccggc ctggagcaag cccgcgctcg
     5941 ttatcatgcg cgtgtggcag atgagcggtt attatatgct gatgttcctt gccgggctgc
     6001 agaccatccc ggagacattg tacgaagccg cggatgtgga cggcgccaca ggatggcaga
     6061 aatttatccg cattacggtg ccgatgctct ccaatacaac gtttgtggta gccatcctgc
     6121 tggtgatcga nnnnnnnnnn gggcgggccg ctcggctcca ccagcaccat catgtactat
     6181 atctatgagc agggctttac caactacaac atgggctatg catcggcgct ggcgtggata
     6241 ttctttgcca ttatcatggt ggtgacgctg atccaatacc gcttccgcca tgaacagggg
     6301 ggtgaatgag catgaaaaaa ccgcagcttg gcatgtccgc gcgcaagacc gtgggcaaca
     6361 ccgtatacca catcttcatg gtcaccctca gcgtcatcat gatctatcct ttcgtctggt
     6421 cgctgctctc ctctttcaaa tcctcggagc agctttacgg cggtaatccg ctggacctgt
     6481 ggcccaggcc gttcacgatg gagaactacg agcgcctgtt ccaggtgctg ccctttgata
     6541 agtttacggt caattcggtg tttttgtccg tcgcgatgcc tctttgtatg atcgcggtcg
     6601 cctctctcac ggcatatgcg ctcacgcggc tggagttccg cggcaggaac attttgtttc
     6661 ttgcgttcat cgccacgatg atgatcccca gccacgtcac cctcatcccc aactataaga
     6721 tcgttgtgga tatgaagctc atcaatacat atgcggcgct gttcctgacg aatatgttca
     6781 cgggcgccaa tgcgttcaac atttttttct tccgccagtt tttcctcagt attcccaagg
     6841 acttggaaaa cgccgccatc atcgacggct gtacgcggct gggcgtgttt ttccgcatcg
     6901 tgctgccaaa tgcgaagccg gccattgcca cgacggccat cctgtcgttc cgcagtgtgt
     6961 ggaacgcgtt cctgtggccg atgctggtga tcaacgacta cgacaagctg acgctgaccg
     7021 tgggcctgaa atacctgaag gagtgggaac ccaactgggc cgtgcttctg gcgggcgcgt
     7081 ccctcagcat cgtgcccatc gtcattgtgt tcctcatctt ccaaaagcag ttcatggcct
     7141 ctaccatgaa ttcgggcttt ggcggtaaat gatgtctctt ttcggaatga acggcgcgcc
     7201 gcgccgtgac atatcaaatc atacaatgga ggatcaacat gaaaaagtca attagcaaag
     7261 ttctgtcact tgttcttgca ctggcgtttg tgttttcgct ggccgcctgc ggcagtgcgc
     7321 cgtcctcggc gcccgctgcg tccggcagcg ctccggcttc cggctccgag cccgcggccc
     7381 cggccgaccc caccacactg aaggtctata cctggtggga cgtgacgaag tttgaacatt
     7441 tgcagaagat gcagcaggat tttgaagcgg cgaatccgga tatcaagctg gaattcgtta
     7501 ccattcccag caaatacgcc gatacaatgg tcacaaagct ggccggcggc gagatccccg
     7561 acgtgatgat gctggccatg gaccaggtgc cccgctatgc gctgaacggt atgctgcttc
     7621 cgctggacga tttggccagc caggagtata aggacgctct gtaccccgtg gtgaaggacg
     7681 ccctgaccgt gaacggcacc atgtatgccg cggcccgcga cgttaccccg aaggtcatgt
     7741 atctcaatac caagatgttt gaagacgccg gcatcgagat cccggccgat acctggacga
     7801 tggacgagtt cgtcgagatc gccaagcagc tcaccaaggg cagcggcgcg gacgctcagt
     7861 ggggttatta ctggaagaat tataccgacc agaccttcgc catgatcgcg gcgttcggcg
     7921 gtgagctgta cagtgaggac ggcaaggcga gcgtgctttc caccgacgaa aacacccaga
     7981 aggccgttca gttcatgtat gacctgtaca atacctacaa ggtgtgtccg tcggccacgc
     8041 aggccgcgca gttcggcgac agcgagtttg ccccctttat ggccaataag gtggccatgc
     8101 agatcggcgc gctttccacc gcctctacgt ttgacgcaaa cggcactgag tataccgtgc
     8161 tgcccatgcc ctatgtggac ggcgtcagca aaacgtcctc gtttgtgaac acctgggtca
     8221 ttcccaagac cgccagaaat cccgagcttt cctggcgcgt ggtggagttc ctctccggca
     8281 aggagggcca gcagatcgct ttggatatga actacggcct gcctgcctcc acgatggtgg
     8341 ataccacgga attcgaggcg aaaacgccgt acaacaagta tttcgtacag gccctggaga
     8401 cggccgtgcc gtatcccacg aacctgaacg gctcggagtt ccagaatatg ttccagaagg
     8461 aatgcgagtc cctgtgggcc ggcgccgtct cgccggagga gtttgcacag cgcgtggatg
     8521 aacaggcggc tccgattttg agcaaataaa gcaatggggg aaagggcggc ggtttccgcc
     8581 gccctttttc aggaaggaag gtcaaggatg accgcaaaac aattgccgga cgatacatat
     8641 ttggataaaa tatatacgat gctggcacag atcgagcagg aggaaggcgc ggccatggac
     8701 gcagccgccc gagcggcgta tgcgtccata gacggcggcg gcctgctgca cgtgttttcc
     8761 acgggccatt cccacatgat cgtggaggag atgttctacc gcagcggagg gctcgtaccg
     8821 gtgaatccca tcctctcgga cgagctgatg cttcataccg gcgcgatcac cagcacacat
     8881 atggagcgca tgcccggcaa agcggccgag gtgctgggca aggcggggct gcgcgcgggc
     8941 gacacgatcc tcatttcctc caatacgggc atcaacacgg tgccggtgga agcggctttg
     9001 tacgcaaagg aacgggggct gacggtggtg tgcgttacct cgaagaaaat atcccgcacg
     9061 cttgcctccc gcgcgccgga gggaaagcgc ctgtatgaag tgagcgatat cgtcatcgac
     9121 aaccatgcgc ccagaggcga cgggctgctc acgatccccg gcacccagca gatcacaggc
     9181 ggcgcgtcca ccttcggaag cctgttcatc gcgcagcgca tcgtactgaa gatcgaaaac
     9241 ctgtatcttg cgcaggggcg gatgccgccg gtcctgtgca gcgccaatct gcccggcggc
     9301 gacgaataca ataccagcgc ggtggcggcg tatcagggcc gtatcaaagc gctgtgctga
     9361 ggatgaggtg aacggtttgt atctggtgtg cgacggcggg ggaacaaaga cggatttcct
     9421 gctgtttgaa aaaacaggat gtgtgcgggg ccgcgcgcag ggcgcaggcg cgaacgcaaa
     9481 ttttgtgcct ccggccgaag cggcgcatac cgtctatgcg ggcgttatgg aatgtctggc
     9541 gcaggcgggg gccgcgcccg cgcaggtgcg ggaattcgtg ttgttcatcc ccggcttccg
     9601 gcccgcgctg ccggaactag agactctttt ggggagcggg aacgtccggc agctgggcga
     9661 tgaaaagaac gctttttatg cggcgctggg cgcgccctgc ggcatcgccg tgctcagcgg
     9721 tacaggctct tttgccaccg ggcgggacaa agcgggacgc acggccacag cgggcggctg
     9781 gggcccgctg ttcagcgacg aaggcagcgg atatcatata ggcgttttgt gcctgtcgcg
     9841 tctggcgctg ctgcacgaca cgcatgtgac cggcacgctt ctggaaaaaa acgtgctgga
     9901 aatgctgggc ctgccggatg ttctgtccat tcgcgacgcg gcataccggc ccgattttac
     9961 gcgcaggcat gtggcgcggc tgagctatgc tgtggaacgg tcggcccggg agggggatgc
    10021 aaatgcatgc gcggtgctgg atgaagcggc ctgtgcgctg gcgcgcctgg ctgcgacggt
    10081 ggccgggcag ctggacgcgg acggcctgcc tgtggcgctc acgggcggcg tggcgcgcat
    10141 gggagagctg ttcaccggac gcttccgccg ggcgctggaa catctggtgc cccagtgcgt
    10201 ataccagccg gcaaagtaca gcccggtggt gggcgcggcg ctttgtgtgt tgagcgagag
    10261 cgcgggcgtg gatatcacgg cacccggcgt cgcggaaaat ctgatgaaag aaaagatggg
    10321 tgaagcacat gttgatggat gaatatttcg gtgcaatgaa gggcgttctg gaaaaggtgg
    10381 agcagaccca gcgggaggcc gtactggagg cttcggcaga gatcgcggag cgccttgccc
    10441 gcggcggcgc gtggcatatt atggacaccg gccatatgct gatgtttgag ggcgtgggcc
    10501 gaacaggcgg tatgatggcg gtaaagccca tccgcatcac ctgcgagata cagaacccgg
    10561 tgcggcaccg gcccgcgcct gcgcggggca tcgtgggcta cgacaccatt cccggctttg
    10621 ccgattacgt gctgggccgt gctaacgtgc tgccggacga cgtcattatg atcggctccg
    10681 tttccggtta ccagtatttt cccgtggatc tggcgctgaa ggcgaatgaa atgggctgcg
    10741 cgaccgtggc tgtcacatcg gtggaatatt cggggcatct taccggcaag caccccagcg
    10801 gcaagcgcct gtttgaggca tgcacctact gtctggacaa ctgcaccaat tacggcgaca
    10861 cgctggtgga tgtgccggcg ctgggccagc gcatctgccc ggccagcggc atcagcgcca
    10921 gctacctgat gtgggcggtg cagagcacgg tggtggagaa actgctggaa aagggcctta
    10981 agcccagtgt gtatatctcg aaccatatgc cggacgcggc gcgcatcaac ggcgaggcgt
    11041 tggaaaatta tgaaaaatac ggtttttgag gggcctgtca tggaactgaa aacaaacgct
    11101 ttccgcgccc ggtttgcgcc ggaggcaaac aatttcgtca gccttgtaaa ccttgcgaca
    11161 ggggacgatt atatcaaagc cgcgccccgc ggccctctgg tggagctgta cgcgctggcg
    11221 gacggagaaa agaaaaagct gctgcccggc gtaccgggga tgagtgcagg agcaggcttt
    11281 ctggagatat cctatacgga gttcggcggt ctgcccattg gtatgactgt gcgctgtacg
    11341 gcggaaaacg accgtctgtg catccgtgcc cggatggaga accacagtgc ggcggatgtt
    11401 gtggaggtac tgatgccgca catcggcggc gtctatctgg gcgaggacta cgccgatgac
    11461 gccatcatct acccgcatca cgcgggcgag cgcacgcgca acccggtaat gggctatggt
    11521 gtgaataaaa aagatttctg gcgcgccagc agcgttgcgt tcggggacat ctaccgccgc
    11581 gagatcaact actgcggcct tgcgtccatg agctggatgt actattacga cgccgaaaac
    11641 ggcctgtaca tcggcagcca tgacgcgcgc ttccccgtga cgggcgtcat agccgaaaca
    11701 agcggaagcg ccgaagatcc gtggatggcc tttggcttcc gcaagcatta ccg
//
LOCUS       DAWBSB010000048        11752 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1296_length_11752_cov_3.277336, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000048 DAWBSB010000000
VERSION     DAWBSB010000048.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 11752)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11752)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11752
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1296_length_11752_cov_3.277336"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4913..4922
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8034..8043
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10521..10530
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2532 a         3192 c         3612 g         2386 t
ORIGIN      
        1 gttggcggct ggcaccgcac gcaatatgaa gaatatgcct tgcttcgcgt ggaagcgtaa
       61 catgcggtcc tgccggaaac ggcaggcctt tctttgtata atctgcacag cgcggcggca
      121 aattgtgcaa aaaaaggaca cgctcagcgc aagcgggctc gcagcgcaca tgttacaata
      181 aaaaggaaaa cttcggccct gtgcgggcct tggaacaaag aaaaaaggag aatctgcgat
      241 gaaatttctg aacgtagaga tcccggttaa gaacttgccg gaactggaca gcagttttgt
      301 gccgctgggc ctgtttttca aagagtatca gaagggggca aaaaagccgc tttgcatcgc
      361 ggtagagcgc tcgggcgggc agattggcac ctatgagaca aaggtgtacg gcacgccgga
      421 catggccgag gcggacgcct attatgtgga cagactggtg aaattccttt tgtggagctg
      481 gggcggcttc aaggtgagca tctgcggcga cgacaacgtg gccgcggcca tccgcgccgc
      541 gtacagcgag ggcggctccc gcgcctttga caagggcttt atggaacagg tgtacgagac
      601 ggagttcatc gtcgagagcc tgccctacga caaaaagccc gaggcagtgc agcgcgccga
      661 atccatcggc cgccacctgg acggcgggcg catcggcttc gacgccggcg gctcggaccg
      721 caaggtgtcc gccgtgctgg acggcgagcc catttacagc gaggaagtcg tatggttccc
      781 caagacgatc tccaaccccg attaccactt tgaaggcatc atggcggcgc tgtccacagc
      841 cgcggccaag attttggaaa agggcggcca tgtggacgcc atcggcgtgt cctccgcggg
      901 cgtgtacatc gacaacaagt gcatggtcgc cagcctgttt ttgaaggtgg gcaaggacga
      961 gttcgacaag aaggtcaaga acatttacac ccgtgcggcc gagcagcttt ccagccagct
     1021 tggttatcac atccccgtgg tggtggccaa cgacggcgac gtcagtgcgc tggcgggcgc
     1081 catgggcctg ggcgaaaacg gcatcatggg catcgccatg ggcaccagcg aggctgtggg
     1141 ctatgtggat accgagggca acatctgcgg ctggctgaac gagcttgcct ttgctcccgt
     1201 ggacggccag ccggacgcca tggaggacga gtggagcggc gacatcggct gcggcgtgaa
     1261 atacttcagc caggacggcg tcatcaagct ggccccgcgc gcgggcatcg agctgacggg
     1321 cgcgtctccg gctgaaaagc tcaaggaagt gcaggccctg atggccgcgg atgacgcccg
     1381 cgccaaggct gtgtacgaat ccatcggcgt atacctgggc cacacgctgg gcctgtacgc
     1441 catgttctac gacatccgcc acgtgcagat gatgggccgc gtgatgagcg gcaagggcgg
     1501 cgacatcatt atggaaacgg cctcccgcgt aatggacgag gaatacccgg acgtggcctt
     1561 ccgccccgaa gcacccgacg aaaaaacgcg ccgcgtgggc cagagcgccg cggctgccag
     1621 cctgccggag ctgaaataat tccggcttgc cgcataataa aaaccgcgcg aaggatttat
     1681 ttcctccgcg cggtttgtta taattgggaa aagcggatgt gaaatgctga aggaagcgcc
     1741 ggcggccgct ccgccgttca aaagaaaaga ggatatttat gctgtacgat acgctggaaa
     1801 atctgaacca gtacacgggc ctgttcccaa acctggacaa agccattgat tttattgagg
     1861 gcaacgacgt ttccgcgctg cctttgggcc gcacggagat cgacggggac gacgtgtacg
     1921 tcacggtgat ggaggccgag cccacgcccg gcgagggacg cgcattcgag acgcattccc
     1981 gctatatgga tcttcagatg gacctggagg gtgcggagct gtgcgaggtg gcgctgggcg
     2041 atgtggagga agcagagccc tatgacgagg aaaaggattt tgcgctatgg aacggagccg
     2101 ccagcgccgc gttggtactg ggagagggcc ggttcgcagt gtttatggtg gaggagccgc
     2161 ataagcctgc cattaaggcg cagggctgcg ataaggtgaa aaaagcggtg ttcaagatcg
     2221 catactgaac cggagggtgc aaaacagccg caggttgttt ggagctggtc cggcggggcc
     2281 gccggaaggg cgcttaggcg tgcggcacga aaaagaacgg gaggcattgc gggatggaga
     2341 gctttatcat cggcatagcg gggggcacgg gcagcggcaa aacgacgctg acaaagcggc
     2401 tgaaggagcg cttcggcgac gacgtttcgg tcatcagcca cgacaattac tacaagcgcc
     2461 acgatgagct gccctatgag gagcgctgca ggctgaatta cgaccatccg gacgcattcg
     2521 atacagatct gatggtggag caggttcagg cgctgcgccg gggcgagacg atcgcctgcc
     2581 cggtgtacga ctttacggtg cacaaccgtt ccgaggacac gctgctcatt gcgcccacaa
     2641 gcgtcatcat ccttgagggc attctcattt ttgcggattc caagctgcgg gatctgatgg
     2701 atgtgaagat atttgtggat accgacgcgg acgtgcgtat tctgcgccgc gtggtgcggg
     2761 acgtgaagaa gcgcggccgc acgctggaga gcgtggtgca gcaatatctt acgaccgtca
     2821 agcctatgca cgagcagttt gtggagccga gcaagcgctg tgcggacatc atcgtgcccg
     2881 agggcggcaa aaatctggtt gcactggata tgatcatcca gcgggtggaa caccatatcc
     2941 gcggccagca ggagccttga acgggacagg ccgggacgga ttttttccgc cccggcaaaa
     3001 tttttgcttg actctgacgg tgcgtcatag tgtattgtag cttttgggag gcatggatgc
     3061 ggcggcacac cgccgcctgc ctcccggaca gaaaggagga acagccatgg aatacaccgt
     3121 aaacggcctt gcacggctgg cgggcgtgag cgtacgcacg ctgcactggt acgatgagat
     3181 cggcctgctg cgcccggccc gcgtcaccga agcgggctac cggatgtacg gcccggatga
     3241 ggtggatgcg ctgcagagca tcctgttcta ccgggcgctt ggcgtgccgc tcaaacagat
     3301 cggtgcgctg atggcggacg cctccgccgg acggctggcc gctttgcaga gccatcgcgc
     3361 cgcgctgctt tgccggcgcg cgcagctgga cgccctgctg gccacgctgg acaaaaccat
     3421 tttgacggaa gaagggaaat gtgcgatgac agacgcagag aaatttgagg gcttcaaaca
     3481 gaagctcgtg gcggaaaacg aggccgaata cggcgcagag gcaaggggca aatacggaga
     3541 cacggccgtg gatgcctcca acgcgaagct gatggggctt tcgcccgagc agtacggcga
     3601 gatgcagaag ctgagcgcgc ggatcaacgc cgctctggcc gccgcggtgc gcgcgggcga
     3661 agcgccggac gggcccgagg ggcggcgcat cgccgggctg cataagcagt ggctgtgctt
     3721 cacttggccc aagtatacgc ccaaggcaca cctcggcctg gcagaaatgt atgtggcgga
     3781 cgaacgcttc actgcctact acgacgctga aacgccgggc tgcgcggctt ttttgcggga
     3841 cgcggtggcg gctttcacgg gctgctgaga cggtgcggcc gcagaggccg gcataaaagc
     3901 aaccgcaaag cggcaaaaaa cgctccgggc gcccggctga acgatgccgg ccgcgcccgg
     3961 ggcgctttgt ttgaaagcac ggcgcgtctg tttacgtgga ggcacctttg ccgtgcaggc
     4021 tttttgttcg tttgcagtcg taaataaaaa agcgcatgga agcggctttc ccgttgatgt
     4081 ggaacagatg ctccctttcg cagcggcgcc gcatgccctg gtgttcgtaa aagccgtagt
     4141 tgcagcttgt gtcggtgaac aaataaattt cgtctaattt ttgttggccg gtataatcga
     4201 gagccgcctg aaacagcgct ttgcctacgc cttgtccgcg gcacgaggga ctcaccacaa
     4261 acaatgcaag ctccgccgga tagttcttgc cgctttcgcg cagcaattcc ctgtctatcc
     4321 ccgtgacgtt ttcaaaaatt ttggatacgg cccggccctc tttggtgaga tagagcgaca
     4381 gaacggcccg aagctgccgg aatctgtttg gaagcggaca cgtgtgacgc gcgatatcct
     4441 ttaccagaat aatgcccacg ggtttgccgt tcagcacggc cacccgggag aatgtgtaat
     4501 tggtcagaca actgctcaaa aatacgtctg ccagccggga cgcggttttt gggctgctga
     4561 attcgtcgta gcgccaggtt ttgcggatga tgttttttaa agcgataaag tcattttttt
     4621 gatattctct caattgcagc ggcatgaagg gcctccttgt tttatatctc ggtatcctat
     4681 aagataaagg atacagtaac tgtaaagtca acagggagag ccgcacaaat gaagaaaagt
     4741 tttaaaagcc tgacaacggc ccagtttgca aagctgcacg gggtgaacaa gcgcacgctg
     4801 cactactatg atgacatcgg ccttttcagc ccgcgggcaa agggagaaaa cggttaccgc
     4861 ttttacgacg ccgcccaaag cctggatttt gaatacatcc gcatgctcaa ggnnnnnnnn
     4921 nngatcaaaa agctgcggca cgcccaaaag gtgctgcgca caaaaaagga acaggccgct
     4981 ttttgcggag agctgcggga acgggagatc agaatagaag cgtgcagggc cgagaggctt
     5041 ctggtactgc cctatgactt tgcagaggac gacatgcagc gggtgttcgc ccatgccaaa
     5101 gagaaatgga gcatagagca gatccgtatg ggcatcggcg gttttatttc gctggacaag
     5161 gtgtacggcg gaaactttga ggcttatgat ggaatctata cgcccgcgct gagcagcgcg
     5221 tctgcggcgg acagcctgac gcgcccaaag gggaactatt tgtgcggata tcagaaaggc
     5281 gtttggagca ggctgccgct gctgtacgag gcaatgaccg aatatgcaga acaaaatggc
     5341 ctgacgcttt ccggatatgc ctacgaggta gggatgaatg aattcatgat ctcagcccca
     5401 gacgaataca ttacaaaggt catgattttg atacaggaaa atgtatgacc gcaaggaggc
     5461 aaagcggggc tggagcgcta ttcctccagc agcgtgaccg tctgcgcgtc tgcatccagc
     5521 cggacctttg cgccgatggg gaccgtgagc atggggtggg tatggcagca gtcaaattcc
     5581 gccagcacgg gaaaatctac atctcccatc acctcttgaa gaatctcata gggctggcgg
     5641 cccgtgccca tatcgtcgaa tttttcatgc ttgcccaaaa taagcccgga gatgcgttca
     5701 aaaacgccgg cgcatttcag cagtgaaaag ctccgctcca ccgtggccgc gtccttgagg
     5761 gaatcttcga tcatcaggat cgttccgtcc gtgatctccg gaaaatattt tgtgccccag
     5821 atcccctgca tggtgttcag attcccgccg atgagcgtgc ctgtcgcggc tcccgggtgc
     5881 agcgtcaaca gcgcattgac gcgccctttt ttggctttat cctgggaatt ccagtcgata
     5941 aattcgtcgg tccactgcgg gggcgtgggc agggtgtgag gaagggtgag ccgttctgtt
     6001 acaatgtcgg taaaatatgt ataggtctca tccacaaacg gtgggaattc gccgaaggag
     6061 gccaccacgg cggggccata ataagtcgtg agcccggtct gggcataaat gcccagcagc
     6121 agcgccgtca catccgaata gccgacgatg atcttcgggt ctttgcacag gccctcatac
     6181 tccagatacg gcagcaggga gttggaattg ttgccgccga tggccgccat gatgcaccga
     6241 acgttcgggt cgcggaccaa cgcgttgaat tcctccgccc gctcccggat cgttccggaa
     6301 cggtaaaaat cccgccgacc tgtcagagaa ccctccaaaa agcgaaagcc tttggcttcc
     6361 agatattctt tgccgcgccg gtagcgcgcc gcggccgtcg ccgtggcggg ggacgagggg
     6421 gaaaacaccg cgatggtgtc ccccgggcgg agctttgcgg gtatcatgag cattccctct
     6481 ctttcggtaa aatgcaatgt gtgccgcgcc gacggcaggc atcaaccggt atattatagc
     6541 acggggagct tttgagcggc aaggcggccc ggacgcaggg ggtcacgcgg cgtcatcttc
     6601 cggccccgct ccgccgtccg gcggaacgcc cgctgcttct tccgggccgg ggcccgccgc
     6661 cgcgctgccg gagcctgtcc cgccgcctgc cgcagacgga gagacgggct gtccgtctgc
     6721 gtctccagcc gcgccctgag gcacactgtc cggggcggcg gatacgccgt cgccgctcca
     6781 cgcactgtac gaggaggcgc gcttgaagta ggccgccgcg acagtgacgg aattttccgc
     6841 aagctcatag cgcccgtccg cccatgtgcc gggcagtacg cccgcgcggt attggggagg
     6901 ctccttgcgc aggaaaccga aaatacgctc atagtcgaag atctgggtgg ccgtcaggtt
     6961 ggtggagctc tgcgccagaa ccgcgcgcac ggcatccggt actgtgtctg ccagcgcgtc
     7021 gccgccgatg cgcagaaagg caagataccc ctccgcgcgc agcagtgcct gcatgtccgg
     7081 cgtagccgcg cccgcagcca gcacgtctgc cagcatctgc ggcgtcagcg agaccgtctg
     7141 taccccgggg atgttcaatt gcagctgccc cagggcctcg gacgtcatgt aattggccag
     7201 cgggatgcgc gcggtaccga agcccgccgc aagatccacc agacggtccg cggtgcagta
     7261 aaggtaattg tcgatttcaa tgtccagtgt ttccatcaaa gccgccgcgg cctgggccgg
     7321 gcccgcctgt tcggccgcat ctgccagcgt ggcgggccgc ccgttcacaa gcaccacggt
     7381 ctcgccgggg atggccaccg agcacaaatt gccctccagc gcatcgaagc gcagcagcac
     7441 aaaggtctcg ggctgctccg gcccggtcat cagcaaaaag tttttttcat cgcccagcgt
     7501 gggctgcgca atgggcacgc cgctttgtac agtgtcgacg ggcgcgctgc gcgtaaaaat
     7561 gacggcggcc accgccagat agatgggcag cagcacggcc agcgtgcagc ccagccccag
     7621 ccagaacgct tttgctctgc tcacgtgcgt gcctccttcc gttttttgta tatatcttgg
     7681 gcgtccggcg cactttatat acaaaaagcg ggagggagaa cggtatggaa aagaacacgc
     7741 agccccagcg ggacgctgcc gcgcgcatcc agaacccaac ggtgcgggcc attgtgctgc
     7801 agccgcccag cgggacggac cccagcggca gctatacggg caagccgctg gacgaaacgg
     7861 aaaggcccgt gcaggacgca gacgatttgt agcacaggca gaaaaccgcg ggcggtgcaa
     7921 tgcgcaccgc ccgcggtttt tcttttgagt aaggaaacca ccagcaatgc cggtggagaa
     7981 accccgtaaa aaagctttag cttcaagcgt tgagcgaagg agctgttaat agcnnnnnnn
     8041 nnncttagct tttttgtttt gcaatttgtt tgccgactgt taagccgggt tcgtttttta
     8101 ccctttctta tcggcatttg ccaaatggcg gcaagctgct ttttacagct tttcgcagtg
     8161 ctcccgaatg atggcgcagc ttttggcaaa gccctccgcc tcgtcgcggc tcagcgtcag
     8221 ttcgatgacc tcttccacac ccgcagcgcc cagaatacag ggaacgctgg cgtacacgcc
     8281 gtgctggccg taaggtccgt ccagcagcgt ggaacaggga agcacggcct tttcatcgtg
     8341 cagaatggct ttgatgaccg ccgcagccgc cgcaccaatg ccaaattctg tgcagccctt
     8401 gccctgcacg atgtcccagc cggccgccac ggtagagcgc caggctccgt acagatccgc
     8461 ctggccgcgg gtgtccggct gctccgccat ccagtccgtc agcggcttgc cgcacacagt
     8521 ggcggcgctg aacgccgcga tttggctgtc gccgtgctcg cccagctcat aggcgcagac
     8581 gctgtgaggc gaaacgccca gcatcgccgc cagacgtctg cgcagccggg cggagtccag
     8641 agccgtgccc gtacccacca cgttcagccc ggtgatgcgg gcaaaatact gcgcgatgac
     8701 atcgcagggg ttggagatgg agactacgat gcccgaaaaa ccactggcgg taagctgcgg
     8761 ggcgatttcc gcaatgacct tgaccgtcgc gtccagctcc agaaggcggt cctgttcaaa
     8821 aatggtgctg cacgcgctga gcaccacgat atccgcgtcc gccagcgctt catagccgcc
     8881 cgcgtatatt tttgcgggca gcggcatata ggccacgctg tccgcgatgt cgtcggcatg
     8941 gcggcgcgcc ttgccctcgt ctgtgtcaag cagcacgatc tcatcgcaca ggccctgggt
     9001 catcaggctg ctggccacgt ggctgcccac atgcccggcc ccgatgatgc cgattttcgt
     9061 cgtttttttc atatgtccca tccctttttc tgttatgaaa aaatcttaca cgcaaaccgg
     9121 agcctcgtca agcggagaaa agagaaataa tcccccgggc ctgtgcgcat actatcccgg
     9181 aggcggggcc gcgtgcgccg cccgcaaagg agttggaatg atgcaggcgt ttttaaagca
     9241 gcacagatgg gtgatcctcg ccgcgggcgc ggccatccag gtcctgacgg gcgtgcccgc
     9301 ggcctggggc gtgtttcagc gcggcgtgtg cgagggctat tccctttcgc aagagaacgc
     9361 ggccatgatt ttcagcctca ccatttgttt tttcggcatt gggtgtatcc tgggcggcct
     9421 tttacaggac aaaaaggggc cgcgcacggc cgggcttgca ggcgcggtgc tgctggcggc
     9481 gggcttctgc ggcgcgggct tcatcccggg ggaaaatccg tggttctttt acggtgtgtt
     9541 cagcgtgcct gtggggctgg gctgtgcgtt tttgtatcct tcggtcatgt cctgcgcgca
     9601 gaagtggtat gcggaccgca agggcctggc cacgggcgtc atcggcggcg cggtgggcgc
     9661 gtccggcgcg gtgctcacgt ttctgggccg ctttctcatc ggcacctgga gcatccgcac
     9721 cgcgttctgg gtgctgggcc ttctgatgct ggcggtgtgc ggcgcgggcg cggctatttt
     9781 ggaaaacccg gagcagccgc cccgggaaac agcgaagcag aagcgcggcg gagggccggc
     9841 acccaaaagg ccgcgggact acagcgtaaa agaaatgctc aaaacaaacc aatactggct
     9901 gatgttcgcc gtagtggggc ttgcaacgcc ggccgtgctg ctgttcagcc ccatcatcgt
     9961 ggagctggca caggagcgcg ggctttcgca gacggcggcg ctggcctgca tcgtggtggg
    10021 ctcggtcttt tcggcggcag gacggctttt gatgccctgg ctcagcgata agatcggacg
    10081 gcggtatacc gacatgcttc tgctggctgc gctgtgcgga ttttcggtgt ggttcatcta
    10141 tgcaggcagc tggtgggtga tcctggtgta cagtctgctt acgttctgct attccggcga
    10201 ggccgctgtc attcccgcgg ccggaacaga cctgttcggc cagaagaacg cgggcatcaa
    10261 ctacggcttt ctggcgctgg gcatgagtgt gggcagcgta ggcttcccgc tgctggcccg
    10321 ctgctttgaa ggcgaggccg tgcggcattt cattgccatt gcggccacgg cggcgggctt
    10381 tgtgtgccta tggtttttaa agcccaccca gggcgaacgc ctgtaagagc gcctgtaacg
    10441 ggagagggga aaagggagac gccttttttg ccctcccctt tttttatgtt ttgcggcgcc
    10501 gcgttcaggc atgggaaagc nnnnnnnnnn gcaccaaaga tatgatgctg caccatgttt
    10561 ctctatgaaa ccgtcaatat gtttttccgg tttgttacag tatgagacat aacgagacta
    10621 attaggacac aatacataat gagtgtcacc tcgcacagtg taatgacatt ttgtacgata
    10681 caacccgcgc caatgctaat gaggattata taaccaagac atatttttta tattgtaaca
    10741 ggcaaggtag gagagacaat tgtgaacttt cggcgtggca attgactttt ttaaatgaga
    10801 atggtacaac atataatgta tatttgtatg aaattctatg aaaggggctg acaacatgaa
    10861 aaaaaataag ctggtagtcc ctgttttgaa atgggtggga ggcaaaagac agcttttaga
    10921 aacttttcgc ccgttgttgc caagcagaat tacaacatac tgtgaacctt ttattggtgg
    10981 aggagcgttg ttgttccatc tccaaccaag tgttgcatgt ataaatgata taaatccgga
    11041 gttgattcgt gtttatactg taataaagaa tgacgtggaa gcattgattg cagaactgga
    11101 aaagtacgaa aacacttccg aatattttta tgccgtgaga gactgggatc gtgataaaga
    11161 gtattatgca aagctatccg aagtccaaaa agcggcgaga gtgctgtatt taaataaaac
    11221 ttgttttaat ggactttacc gtgtgaaaaa cgcaggggag tttaattcac cgtttggtaa
    11281 ttaccgaaat cccaatatcg ttaatgcgcc tgtcctgcgt gcagtcagtg catattttaa
    11341 cacagccgat attagtatga caacagttga ttacgaggat gttctgaaaa ccttaccaaa
    11401 aggagctttt gtatacctcg atcccccata tgacccggtt tcagaaacct ccagttttac
    11461 aggatattct aaatgtggct ttacccgcgc agaccaaatt agattgcgta aatgctgtga
    11521 cgaactatct gtccgcggca taaaatttat gctttccaat tccgcaacgg gctttattat
    11581 ggagcagtat gctgcatata atattacagt tgtacaggca aagcgcgctg ttaatgccat
    11641 agcttcaaag cgtggagatg ttgacgaagt ggtggtgcgt aattatgagt gagcgtcaga
    11701 aaggccttaa tgatattgca tgggaacaat tatttaaaaa gtatgatatt ct
//
LOCUS       DAWBSB010000049        11743 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1298_length_11743_cov_3.311174, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000049 DAWBSB010000000
VERSION     DAWBSB010000049.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 11743)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11743)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11743
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1298_length_11743_cov_3.311174"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5279..5288
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5961..5970
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2174 a         3741 c         3498 g         2310 t
ORIGIN      
        1 cattgttaaa agcgccgcac ggcatgcctg cttacagaga ttcagccgca gcggtgtccc
       61 cagcaagctt cccattgcta aaaaatgcgg cggcggcgag gtccgcttcg tccggatgag
      121 acagcgcgcg gtcgaaattt tccagcatgc gttttgcgtt ggcgttttcc gggtcctgct
      181 ccaattgcgc ggcaaagcgg gcgcgcacgc tgccgggcat ccggccctga cacatccagc
      241 cgcccagata tacggcgtcc gaaggcagca gctttttcac attctccagc agcatattga
      301 aatagtccgg gtccccgccg aaccccgccg taccgaacag cgccaccttt ttgccgccgc
      361 acaaaggcaa aaacccggcc atgtcctgtg cgcatgtgcc cttgtctgtc cagaatccgg
      421 cgaatatcac gtccgcgtca agagcctccg gcgcgggcgc gccgcggtaa acgcagccct
      481 ccggcggcag cgcggcggca atggcgtccg ccacggcctg ggtattgccc gttttactgc
      541 ttgtcacgat ggaatatgtc ataaaaatca aaccgtcctt tcctggtgcg tccctgtgcg
      601 ggcacatccg cggccgcacc gcggccggcc gcctcttctg cttcgccgcc cgtcccacag
      661 gcggctgctt ttattatgta ccccttcccc gcgttttgca accgtcttcc gctgtttttt
      721 cgttgacaaa tgtcacggaa tatgataccg taaaatcaac gacaattgtc aacgaaaaag
      781 gaggggccgg acaatgccga aaaaaccgct tgaaaaactg ccggaggctg agctggacgt
      841 gatgctcgcg ctgtggacgc acacgcagcc cgtgcgcacc gcacgcctgc tggaggacct
      901 tgcggataag gcgtggtcgc tttccacgct caaggtcctg ctgggccgcc tggcggacaa
      961 aagcttcgtg gaagtaacgc gcgagggccg cttcacgctg taccgcgcac gggtgcggga
     1021 ggaagactac cgccgcatgg agacaaacgg cctgctgcgg cgctactaca aaaactccgt
     1081 ggcgggtatg gtcgccgcgc tggtgcagga cgaagcgctt accggcagcg aattggcaga
     1141 gctgaaagaa ctgctgcgaa aggcgggcga gcccggtgct gaatgacctg ttttacgctg
     1201 tgctgggcat gctgccctgg acatgcgcgc tcatcgcggc gctgctggta ctgcggcatt
     1261 ttgcgcgtgc cgcgctgtcc gcacggttct tccggctggc ctggctgatc cttgccgtcc
     1321 gccttgcgct gcctgtcagc ttcgcggtgc cggtgcagga caggcccgcg ccgttgtcca
     1381 tcccgctccc cacggccgcc gtccggcaag cggcgctccc cgccgccccg gcagaaagcg
     1441 gcggcgcaca gcaggcggcg gcctccgggc ccggtaaggc tgtgcccgtt ccttcggccc
     1501 ccgccccggc ggggccgctc cgctcccggc ccgggcttgc cggggccgtc tggctggtcg
     1561 gcgcggcgct gtgcttggcc gcgcgcctgg ctgcctatgg gacgtccatg cgccgtctgc
     1621 gccgtgggcg ggtgccgctt gcggacgaaa cgcttgcgga cgcctgccgc gccgccttcg
     1681 gccggagcat gcgcgtctac gccgtggccg ggctggatac gcccatgctg gcgggccttt
     1741 tgcgtccggc tgtctatctg ccggagggcg cgggcctgcg cgcggacacg ctgccctatg
     1801 tgctggccca tgaagcacgc caccggagcg ccggggatat ccccttccag ttcgtgctgg
     1861 cggccgcctg tgccctgcac tggttcgacc cgctggtgtg gtgcatggcc cgcgccgcgc
     1921 ggcaggatat ggagcttgcc tgcgacgaag ccgtgctggc aggccgtgac atggcttacc
     1981 gcagggccta cggcgcggcg gtgctggacg tgctggcgtc cgcgcgcagg cggcgcatat
     2041 ccgcgctcac caccggcttt gcctccggcg caggagaaac cagacgccgc tttgcagaaa
     2101 tgctcagcct gcgaaaaaag cggcgcggca cgccgctttt gtgccttctg ctggcccttg
     2161 tggtgctggc cagcacgctc gttgcctgcg caaccccggc ccggtcggat accgccgcgc
     2221 ctgtttacaa gggcggcacg ccccagccca cggccggaga agcaccggag gaacggctgg
     2281 aacgcctgcg cgaagaatta cagacgctcc aggcggaaat cggccgcctc gaagcgcttg
     2341 ccgcgcagga tagctcctta gaggaaatac agcggcaata tctgcaggcg cagcaggagt
     2401 gggcagaatg gtccgccgcc gtgcctgaag gcgcgccgtc cggcggcgcg gcccaattcg
     2461 cctggcccct gccgggttac acaacgcttt cctctgattt cggtacggcg cgcagggtat
     2521 acggcgcaaa ggacgtacac accggcatgg atatccccgc cccggcgggt acgcccgtct
     2581 acgccgcggc cgacggagca gccagcctca acaaccactg ggtgatgggc atttccgtca
     2641 agctggatca cggcggcagc ttggcgacgc tgtacggtca tctgtccgcg tgcgcggtga
     2701 aggatggcga cctcgtggaa aaaggccagc tcatcggtta tgtgggcagc acaggcaaca
     2761 gtaccggaaa ccatctgcat tttgaagtgg acctgaacgg cgaccccgtt tcggcgtggc
     2821 cttacctcaa tgccgaatag gaggttttcc gcggatgtac aaaaaaggaa tgcggcgcgc
     2881 cgccgctctg ctggccgttt gctggctggg gctgctgttc cgggcggcgg ccgaaacgcc
     2941 cgccgaaaag ctggagcggc tgcgtgccga gcagcaggcc atcgagcagc agatcgagca
     3001 aaaccggacc gacgccgccc gcgccggaga ccttgtgaac ctgtatacgg ccaaggagca
     3061 gaacgtcaaa gcccagatcg cggtgcttca attgaaaata caggaatgtg aagcacagct
     3121 tgccggcacg caggcccagg tggcagccaa actgctggag gtggaccgaa gcacccaaag
     3181 ctttgaggcg cgcatgaagg gcatgtacga aatgagccgt caatccggcc ttgccgttct
     3241 gctgggcgcg gacagtctct ctgagatgct cacattcgga gaatatttgc agcgcatcgc
     3301 ggtgcgtgac acacggctca tcgccgagct gacagcccgc cgggaagagc tggccgccct
     3361 cgccgcgcgg cagcaggcgg agctggacgc gctggacgca tccaatgcgg aactggaagc
     3421 cgctgcaaat gaatatgccg cggcgctgca gcaggcaaac gcccggcttt ccgccgcgca
     3481 ggcagccgag caggccggcg cggccgcctt tgccgccaag gagcaggaga tcgccgcggc
     3541 ggaacgggaa tacgcggaat gggccgccca aagcgacaac gacgcgcccg aatacacagg
     3601 cggcgcgttt tactggccgc tgccgggcta ttccaccggc tcgcccatgg gctggcgcac
     3661 gctgaacggc gtacaggaat tccaccgcgg catggatatc cccgcgccgc tgggtacaaa
     3721 aatctacgcg gcggcggacg gtgttgtcag cacaaaagca cactacacgt acggcacctg
     3781 tgtcaagctg agccatggcg gcgggctggt gaccatttac gcccatatgt ccgcctgggc
     3841 cgagggcatc gccgacggcg tccctgtcaa gcagggccag ctcatcggtt atgtgggccg
     3901 aacgggccgc gcatatggat accacctgca tttcgaggcg aacctgaacg gcgcgcccgt
     3961 ggacgttcgc gattatctgg ggtagcgcgt tcgtttggcc ctgcggccgc gcgcagaaaa
     4021 gaacagcggc ccgcgcgtcc aggcaacgtg cggccgctgt ttttgtatgc ggaaaaggct
     4081 gttcaaggca acggataaga tatgccgtct atttcaatgg ccgcgactgc ctcagggtct
     4141 aaaatatcct tcagcatata ccctgccaca aactcgcttt tgccccgcgc cccgtccggc
     4201 gtaacgcgcc attccgtcaa ctgatcacca taaatcgtga tgcgggaacc attttgaaat
     4261 acaagctcgg cgttccgcag gtaatgatag aacggctcgt tcatcgcctc ctccggaggg
     4321 ggcggaagct gcctggctgc catattggga tccgccccac tgccggagat gcgcacggag
     4381 acaggagata tccgtaccgt ttccacgcgc atcttcaggt gctgcggcgc gcactcggta
     4441 tgcttgaggt ccgtggatgc cccataaagc ttgaattccg ggtagtatat ccccgcgcaa
     4501 aactccacgg ccgcatcctt cagcctccat tcccgggcag atgctgcggg cggaagctcc
     4561 agaagtatcg tctgcgccgt gcctgggtcg gcttcgctgc acacgcacag cgccagcgtt
     4621 tcggcccatt cgggaatgcg gccccggttg tagaaacggt accagccgcc gcccagctcc
     4681 tgcggcgcgc cgcctacaaa cgaaaaactg tacatcattt cctccggcgt catcgtcagg
     4741 gccgggcatt cgatcaccgc cccgggcgga gtgtctcccg ccgcgagcgg cacaaaggag
     4801 aacttcgagg ggaactcgcc ggcaccatgg tcgacattcg ttgtcacaaa ccggcttgcc
     4861 tcccgggcgc tgtccgccga tacgtcatac agaacaaaag cgttctcctt atcccgcaaa
     4921 agagccgtga tgcgcatggt atggccgtcc acggtcactt ccttgctcgt gtaggccgcg
     4981 ccgtatcgtt ccaccaccgc gtcggcgccc gttcgccgtg tcatgggagc aaaatactgt
     5041 accgcaccga tgacggctgt acaggcgcac agggccaacg cagccgccaa aacggcccac
     5101 aggcggcccc gtccgcgaaa gccgccccgg cgtttttcag gcggagccgc tgcgtccggc
     5161 acgatggcgg gtaccgctgt ggaggccttc tgcgcgccga ggaggttctc aaccgctccc
     5221 tgctccagca cgcggcgcac aagcgtctcc tgcttctcct ggggcagcgg cggagaaann
     5281 nnnnnnnngc aatgcttcgt tcataaggac acctcctttt ccagcaaagc acgcagcttt
     5341 ttgcgcgcac gcgccagctt ggtgcgcacg gtagcatccg gcaggccgta gtgctcggcg
     5401 atggcccttg tctcctcaca gtacaaatag cgccgtagaa atatttcccc gtccggcggc
     5461 ggcagcgcca aaatcatccc cgcaagctcc cgcgtttcca ggaggctttc cagttctacg
     5521 gcaaaatcca tatcttcctc ccgcccgtca agcgggaaca acacaccccg gcgcgtcaac
     5581 gcccggtaac ggtcaatggc gcagcttcgg gccgtacgca ccacataacc ccgcagagcc
     5641 gtgcccgcgg gcaggctgga cgcgttgcgc cacagacgga caaaagtatc ggcggcagcc
     5701 tcgtccgcgt cctcggcgtg gccccgcaag atagccgtgc aaatggcatg cacgggcccg
     5761 ccgaacaatt ccatcagcct gcgcaggccc tcctgcgggt cgttttgcag cagcgcggcc
     5821 gcggcggtgt aatcgtcgtg cagcaaggtg gttcctccct tcaaaagtgg gttcccacac
     5881 agaataacgc cggaaacagc cgttgtgttt caaaaataat ttttgattgc tgcgcaataa
     5941 aaaagctcat cttaatggcc nnnnnnnnnn caggccggga cggcctgcag aaccgaaaaa
     6001 ccagcctgcg gctgtttttt atttctgtga aacgcagcaa atattttcgg cgttgttctg
     6061 tatgaaaaca ataccgaatg gtaccggcct ctttgcttcg cagccggaac agccctcccg
     6121 gcctttctcc cctttgacga ggccttttgg caaaacgaca aattttacag cttcacgaac
     6181 aatctggccc caatactgtt ataatggaat tgtccgactg gaacagcctt ttctctaaaa
     6241 caaaaggaga gcaaagcgac ggataaaatc acgtttcttc ttgaagttga tatagcatta
     6301 tgcttgttgt ggcttgttat ctattactat cgcaggtcaa atcaggacaa gcagcagcag
     6361 gctttcgcgc tgtatctttt ctgcaaacgc caaacgcccc gcaccatccg gtgcggggcg
     6421 tttttcatcc atgcggccgt cttatccttt tgcacgcaaa agcggtgcgg cgggtaacac
     6481 aaaattttct gccaaaaacg cctgtgtccg ctctgtcagt cctgcacagg gaacagcacc
     6541 gtgaaggtat tgccgtccgg cgcggccgtt acggtgacgc tggcgccgta attggtggcg
     6601 atgtgcttca cgatggcaag ccccagcccc gtgccgcccg ccgccttgct gcggcccttg
     6661 tcgatgcggt agaaccgttc aaagatacgc tgggcgtatt ccggcgcaat gggctcgccc
     6721 gtgttgaaca cgcgcaggca cggcgcgccg ttctcgttcg tgaggttcac ttccacgcgg
     6781 ccgccctgcc ggttgtactt gacggcgttg gtgatgaggt tgcccgccag ctcgctcatc
     6841 tcccggtggg aggccgaaag cacacagggc tgcgtggtgc acgagatgct caccttgctg
     6901 cttctggcaa attccgccgc gtccgagcag cactcgctca ccacggcggc aaggtccagc
     6961 acctcccgct caaaggtaat gtcgccgctt tccaggcggc tgatcatgat gatatcgctg
     7021 atgaggctgc tcatgcgcat cgtctccttg aggatacgct tggagatcgc ctcctgcttg
     7081 ttgccgccca agggcatatc gctgcacagc agctcggcaa agcccttgat ggaggtgatg
     7141 ggcgttttca gctcatgtga ggcggattca aagaaatcct gccgcatctt ggccgcgttg
     7201 cgctgcgccg tcacgtccga gatgatgatg atggcgccgc cgtcgatggc gcccgtgtcc
     7261 tcgggcttca cgcggctgat gttcacctcg cccttgcgct tatcgggcag cggcatatcc
     7321 acggccacgt ggccgccttt tttcagcacc gccgccacgc cgtcgatgag cgggatgttg
     7381 cgcgtggcgt gcaggatgtt cttgccctgg gcctcgtttt ttttcacacc gaacgcgcgg
     7441 cacgccgacg cattgatgag caggatgttc tggtccgtat ccagcagcac gaagccctcg
     7501 ttcatgttgt ccaggatata gtcgatcttc gccttttccc gctccaggcg gcgcatgttc
     7561 cgggcgatct cctccgacat gcggttgata tcgcccgcca tggcctgcaa ttcctcataa
     7621 gggtatttct ccggctccag ccgtgcgccg ccctcctgca cgtttttcag gctttcggta
     7681 aaatcggaaa tagggtccgt cacggttttt gccacgcgcg tggccacgaa cgccgccact
     7741 gcaaaagcga ccagaccggc gcccagcaac gcgggcagca ggcttacaag gtcccgcaaa
     7801 aagccgttgt actgcatggc cacacggata taatccccgc cctgcgtgcg caccgccgca
     7861 tacatcaggc gggtgccgag cgtctcgctg gggcgcacgg agatgcccac gcccgcgccc
     7921 tctgccgctt tcagctcctc gcggccggcg tggttttcca tggttttgta atcggcggcg
     7981 gaatcgccca gcaccgtgcc gtcctttgca atgaccgtta cgcgaagccc gccgatctgc
     8041 cgcgaaaact gcgacgcctg cgcgtcggcg tcgccgttcg cgtcataggc agagcccagc
     8101 gccgtggcaa ggctgcgcag ctgtgtctcc gtctgctgcg tggtctttgc gtcaaaaatc
     8161 gccgccgtca gcaaaaggca gcacacaagg cccgcgcaca atacgagcgc cgtgcttttc
     8221 agaatgcttt tcttcatgtc cctttaccct tctttttcca cttcgcccag aaagcggtat
     8281 cccacgccgc gtacggtttt aatgtaccgg ggcgcggtgc tctcgtcgcc cagcttatgg
     8341 cgcagcgtgc cgatgtgcat gtccagcgtg cgcgtttcgc cgatgaagtc gtagccccaa
     8401 acactttgca gcagttcgtc gcggctcatg gcgccgggtg cgttctccat cagctcacgc
     8461 agcagctcaa attccttata ggtcagttcc agcagagccc cgtcccgtgt ggcctcgcgc
     8521 accgcaaggt ccagcttcag gccgccgctt ttcagcacgc cgcccttttt cggcgcgggc
     8581 gcggcgccgc cgcgccgcag cagcgcgcgc acacgggcgc acagctccag cacgctgaac
     8641 ggttttgtca tgtaatcgtc cgcgcctgca tccaggccga tgatcttatc gttttcagaa
     8701 tccttggcgg tcagcatcat tacgggcacg cccgcaaggc gcgcgttcgc acgcatcagc
     8761 ttcagcgccg tgatgccgtc cataccgtcc agcatgatgt ccagcaccgc cgcgtccggc
     8821 acggtattct ccatttcagc cagcgcgtcg gccgcattcg ggaacgcgct taccgtatag
     8881 ccggacgagc gcagcgccac ctccagcagc atgcggatgc tctcatcatc ctccaccaca
     8941 aaaatacgcg gtgccttcgg cataccgatt ttcctccttt tttaacaatg ggaagcgcgg
     9001 ccttacagcc gccgccttga atctcttcgc gcagaatgcc gggcagggca ttttgccgtt
     9061 ttccgcagcc ttacaggatg cgttcgcttt tgtgcatgcc ggttttgtag aactgcaccc
     9121 attcgcagat gttcaccgca tggtcgccca aacgctccag atatttggcg atcatcaggt
     9181 agtcgatggc gcagtccaca tgctcttcat tcacggcgat atacgcggca aggtcttttt
     9241 tgatcttgtt gaaataatcg tccaccacat cgtcccgggc aatcacgtcc gcagccagaa
     9301 ccaggtcctc cctgacatag gactggatgg aatcgtccac cattttctgc gcggcctcgg
     9361 ccatatccgc aatgtcggac aaagaggccg ggatgccgtg ctccttgatg tgcgtggtga
     9421 tctccgcgat atctgcggcg gcgtcgccga tgcgttcgat atccgtgatc atcttgatgg
     9481 ccgtggatat tttccgaagg tcgcccgcca cgggctgctg gcgcaaaagc agcgtcaggc
     9541 actggtgctc gatgctgcgc tccatatcgt cgatgttgcg gtcccccgca ataacgcttt
     9601 gggccagcgc gccgtcgttg gcctccagcg cctcgatcgc tttgtcgatg gcgtccgtcg
     9661 ccagggcgcc catgcggatg agcgacgcgg aaaggctctc cagctctgcg tcgaaagaaa
     9721 ggcgtgcgtt cataagggtt actcctttat tataggttgt ttggcaaaaa agcacaatgg
     9781 cagggcatat ttttttacat gccgccccgt gggaacggct gcaaaacagg ccgcgggcat
     9841 caaccgaagc ggcccgtaat gtagtcctcg gtccgcttgt ccttcggcat ggagaatacc
     9901 tgctcggtct cgccgaattc gatgaggtcg cccaccagga agaacgcgca tttgtcgctg
     9961 atgcgcgcgg cctgctgcat attgtgcgtg acgatgacga cggtgtagct ttctttcagg
    10021 gacgccatca attcctcaat gcgcatggtg gaagccgggt ccagcgcgct ggtgggttcg
    10081 tccatcagca gcacgtccgg ctccacagcc agcgcgcgcg cgatgcacag gcgctgctgc
    10141 tggccgccgg aaaggcccag ggccgacttt ttcaaacggt ccttcacctc gtcccacagc
    10201 gccgcgccgc gcagtgattt ctccacaagg tcgttcagct cggctttgga atgcgtgccg
    10261 tgcagccggg ggccgtaggc gatgttgtcg taaatgctca tggggaaagg gttcggcttc
    10321 tggaatacca tgccgatctt cttgcgcagc caggtcacgt ccacctcttt ggaaaaaata
    10381 tcccggccgt tgtacagcac ctcgccctcg atgcgcacgc cgggcacaag gtcgttcatg
    10441 cggttgagcg ttttcaggaa tgtagatttt ccgcagccgg acggcccgat gaaagcggtg
    10501 atctgttttt cggggatctg catatcgatg cccttgagcg cctcaaaggc gccgtaataa
    10561 agcttgagac ggtttgtctg caaaatgatg gggctttcca tattgttctt actcctcttt
    10621 cactgcttcg cggttacttc gtttttctgc gcagcttctt ttccaccagc ttcgccgcgc
    10681 cgttgatgca cagcaccagg agcatcagga tggccgcgat ggcgaagccc acgtcgaact
    10741 cgccgcgttc gctcacatac atgtacagcg ccacgctgag ggtgccgccg ctgcgcgtga
    10801 aggccgaaaa gatattgttt gccacaacgg tccccacgcc cgccgtaaac agcagcgcgg
    10861 cgctttcgcc cacgatacgg ccgatggaca aaatgcagcc tgttacaatg ccgtccaccg
    10921 aggaaggcag aatgatggtg cgtatcatgt accattttgt cgcacccagg ccaagcgcgc
    10981 cctcgcggta gctctggggt acggtcttca gggattcctg cgtggtgcgg atgatggtgg
    11041 ggaggatcat gatgaccagc gtgcacccgc ccgagagaag gctcgtctga aagcccagca
    11101 tctgcgcgaa gaacagcatg cccacaaggc cgtagataat ggacgggatg cccgccaggg
    11161 tctcggtggc gaattcaacg atgcggatga acgttttatt ggaggcatat tccgtcaggt
    11221 acactgccgc gcccacgccc agcggcagcg cgatgagcag ggtgaacagg atgatataca
    11281 atgtgttcag gatgtttggc agaatgccga tggtatcctt gagcacactg ggcgccgtgg
    11341 agagcagctg tccggtgata tgcggcagac cgcgcacaaa gatatagccg atgagcacca
    11401 ccagcagcgc cacggtgagg aaagcgctca gatataccac tgttttgaaa aagatatcgt
    11461 atgcgcggcg gcggccgctg cgcctgtctt gtccgttcat ggtttctccc ctttcttcag
    11521 gaagaagttc agcaggctgt tgatgagcat gataaagcag aacagcacca gcgcgatgct
    11581 gaacagcgcc tgcttctgca ggccgctggc atagctcatc tccttggcga cggccgtcgt
    11641 caggaaggtg acgctcttga acaggcccgg catattggcc acgttgcccg ccaccatcat
    11701 cacggccatg gcctcgccga tggcgcggcc gatgcccagc acg
//
LOCUS       DAWBSB010000050        11627 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1309_length_11627_cov_3.071552, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000050 DAWBSB010000000
VERSION     DAWBSB010000050.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 11627)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11627)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11627
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1309_length_11627_cov_3.071552"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    8284..8293
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2226 a         3745 c         3320 g         2326 t
ORIGIN      
        1 cggtgaacct ggcctacgag gcggccactg ccgacctcgc ggacgtgaac atgatcgacc
       61 cgttccatct ggaggcctac ggcgtcacca ccgtcaacta caaccgggac gtggaggcct
      121 tccccatcgt gcgcaacatc ctctcgcgca ttacagggga cgaaagcgta tacaaatctc
      181 ccacggatat gggcgtgaac atggcgggct acgccatcgt ggacgacgaa gccgtccgcg
      241 ccgccgccag ccaggagatc atccgccgtt actacaaggc cctttgcgac cacaagcagg
      301 gtaagggctc gcaggacgcg gtggacaaaa tcaagcgcat catgcaggag ctttccctca
      361 ccacggcgga ccgtgcggtg gttgcgcccg ctcttgccaa aagcgaaagc tccggcgtgg
      421 ccgccgcggc catccagctg ccggacggac cgatcgtaac gggcaaggcc agcaacctga
      481 tgagcgcctg ctcctcctgc gtgctcaacg ccatcaaggc cattgccggc atcgacgatt
      541 ctctcacgct catcagcccc atcatactgg agccgatcct caagctgaag attgacatcc
      601 tcggcagccg ttccagcgtt ttgaaggtgg acgacgtgct cgcggcgctc tccatcacag
      661 ccgccaccaa cacgctgacg gcccgcgcca tggccgcgct gccgaagctg cgggactgcg
      721 agatgcacag cacctgcatg ctgcattccg gtgacgaagg cacactgcgc aaaatgggcc
      781 tgcgccttac ctccgagccg gaatacccca gcaaggacct gtttttctga gcaggcagac
      841 ttttcaggag cggcaaagcg gtgcagagac ggggctcctc cacggaaaac accggttttg
      901 gcgcccagcg tattcaaaaa gcctggcgat cctttgcgga tcgccaggct ttttcgcgcg
      961 cttacagccg ctccgcgtcc ggcgcggcct tctctcccag atagtttccc cacgccgccg
     1021 ccagcttatc catcgtccag gcggcgttgg ccgggctggt gctgggcagc ttcacggcct
     1081 ccagcccggg cacgcggctg tatttttcat acagccggta cgccgtggcc ccgttgcaca
     1141 aaacgcgccg gatgggcgct ttgcgggcaa ggcccgcaat atccgcgggc accacgtccc
     1201 ggatggaagc atccgaagcg ccggtgatgg tacagctttc aatggtgtcc cacagcgcca
     1261 ggccatgggt gaggatgagt gcctttttct ccggcacgct ttggggcacg ggcgctccca
     1321 gaagccgcgc cagcagcggc cagaaccgat tctgcggatg gccgtaaaaa aagccctgct
     1381 cccggctttt ggggctgggc caggttccca cgatcagtgt gcgggaatcc ggaccgtata
     1441 cgggcggaaa cgccattttt tcttcctcct ctgcgggccg ggccgctttg cgccggccgc
     1501 gcacagcgcc ctgccgcggg cgtccgcccc tgttttttct cttccctgcg cgggcgcgtc
     1561 accccagcag atattcccga acggcagccg ccgcgccgtc cagggccgca gcgggggcca
     1621 ccgcgtcggc caggggcagc aggctctgcg cgccgccctc cattgccacg gcaaagcccg
     1681 ccgcctccag cagctccgcg tcgttttcac cgtcgccgaa ggccgccgtt tccgcccagg
     1741 agatacccag ccgctccagc agccggccaa cgcccgccgc cttgcctgcg tccgcgcgca
     1801 gcacgtcgta ccggcccggt gcaaagggaa tgaaccgcag gcccagatgg ccgtatttcg
     1861 cttcaaaggc cgctgcatgg cgcgcgtcca ttgccgcgca ggcgccgaag ggcatgtcct
     1921 gcaaatggcg cacctggtcc tcgccgtccc gcagcacgct gtcaaaaaag ccggacgcag
     1981 cgttctgccc gcagtacttt tccacaaagg ccgcgtattc cacatacaca tagtatgcgt
     2041 cggaaaaaat aaattccagc ggcagctcat ggtcctcgca gaaatccaca agcgcgtaca
     2101 tttcctcggg cgtcatgcgg ctctgccaca caggccgccc cgccgcgtcc gtcacgtccg
     2161 ctccgttcac cgccgcgaaa taatccggct ttatcccgct gcccagcacc cactgcgccg
     2221 tataccgcgc gcgccccgtg gcaatgacga cggccacacc gctttgcgcc agctcccgca
     2281 gcgcctgcac cgcggcctgg ccggggccgg gcgcgccata gggcaccagc gtgccgtcaa
     2341 tgtcgaacac agccgctttg atcttcatgc aaaccaccca ttccgccgcc cgcggcggcg
     2401 cagattccgg cggagctttc cgccgctgcg cccatcataa cacaaaacgc gccgcccgtg
     2461 caaaccgccg gacggcgcgc tgcttctgct tttcaggcgt catccctgcc tcgcggcggc
     2521 gcgtcattcg tagcgcagcg cctcaatggg gtccagccgg gccgctttgt tcgccggata
     2581 atagccgaag aacacgccga tggccatgga gaacgccacc gcaatgaaaa taacgcccgc
     2641 gctgggcgcg gccgcatagc ccagcaggtt cgcgcccgcg gcgcccaggc ccaggcccgc
     2701 cagaatcccc agaacgcccc cgatgaggca gatgatgacg gattccacga tgaactgcac
     2761 acggatgctg ccgttcgtcg cgcccagcgc cttgcgggtg ccgatctccc gtgtgcgctc
     2821 ggtgatggac accagcatga tattcattac gccgatgccc cccaccagca ggctgatgcc
     2881 cgcgatgacc gcgatggcca ggctgatggt ggagagcatc tccgtcatgc tctccatcag
     2941 gctctccatg ctggaggccg tggccgtata ggaggtattg cgcgtgtaat agccggcgaa
     3001 gaaattttcc gtctgcgtca gaaatgcgct ggtgtccacg cctgtggccg ccaccaccgt
     3061 gaagctctgg tagccgtcgt ccgcatggga catggctttg gccgtggaga tgggcaggta
     3121 catgtccgtg gtgggggtgg cgtctgtgga ttccagcacg agcccgttgc tctcatacgg
     3181 gtacaccccc acgatataaa atgtgtatac cttggtgccg taggtgagct ggaacgcctg
     3241 gccgatggcc tcctgccctg gaaacagtgt ttcagcaaac acgtcgctca caacggccac
     3301 cgtgcgggct ttttcctcgt cctcctccgt gaagaagcgc ccgcgggaca gctccacgct
     3361 gttggcggcg gcatattcgg tgttcacgcc cgtggcgttc acgccggctt cgtccgcgtt
     3421 tgtgcttacc gtatagctgc caaggctctg tgtcaggctg acagcgtaga tgctgccgcc
     3481 gtaaagcgcg cggtaatccg ccagcatctc atccgtcagc aggtcctccg cgccgggagt
     3541 ggttgggcgg aacatgcgcg tctgcgcacc ctcggtgtaa tcgctgtcgc ttttttgcgt
     3601 caggctgacg gtgatgttgt tcgcgcccat gctctgcatg ctggtggtga tggagttcgt
     3661 catgctgccc gccaccgtca tgatagcgat gaccgagccg atgccgatga tgatgcccag
     3721 catcgtgagc agcgcgcgca tcttgttggc ccgcaagccg ttgagggcaa gctggatgtt
     3781 ttcaaacagc agcatccgca gcgcctcctc ccctgcgcac gcccgtgacg cggccgtcct
     3841 tgatggtgac gatgcgctgg cattcgccgg caagctccgg gctgtgggta atgagcacga
     3901 tggtctttcc ctgctcccgg tgcagccggt ggaacaggtc catcaccagg cgccctgtgg
     3961 cggaatccag cgcgcccgtc ggctcgtcgg ccagcaggat ggcggggtcg ttggccatgg
     4021 cgcgcgcaat ggccacgcgc tgcttctgcc cgccggaaag ctggctgggc gcatggcccg
     4081 cgcggtcgcc catttccacc atatccagca gcgcgcgggc gcgctgtgtg cgcgcagcgc
     4141 cgcgcacgcc gttgtacatc atgggcagcg ccacgttctt cagcgcgctg gtgcggggca
     4201 ccaggttgaa cgtctggaat acaaatccga tcttccggca gcggatgcgg gagagctgct
     4261 tgtcgcgcag tgtgcctacg tccacgccgt ccagcacata ggtgccttcc gtggggcggt
     4321 ccagcgcgcc gatgatgttc atcagcgtgg atttgcccga accggacgcg cccacgatgg
     4381 ccacaaattc gcctttgcgc accgtcaggt caatgccgtg cagcacctcc agttcctcgc
     4441 ccgtgccgat ggggaaccgc ttgacgatgc cgtgcatgtc gatgatggga gcatccatat
     4501 cacatccctc ccgggccgcc gctgggcatg ccgccgccac cgccaccggg catatcgccg
     4561 cccggcatgc cgccgcccat gtccatgctg aacatggcgt tttccccgct ctgctgcgcg
     4621 tcgtccgcgc ccgcggcggc cgtggccgta tcattggcgc tcgtctttac ctccatcccg
     4681 tcggaaagcc cttcgccgga gatctcaata taataatccg tttccatgcc cgtttccaca
     4741 gggatggccg tgaattccgt ttcgcctccg ctgcgggcgt aaaccacgct gccgccgttt
     4801 tcgtccgtgc ccaccgcatc gtaaggaacg gcatagacgt ccgtcacctg ggaaaggatg
     4861 atctccacct tcgcggaaag gccgatgagc aggccgctgt ctttcccggt tacggtcacc
     4921 tcggccccga agccagagct gccggtaccg ccctgcgtac tgcctgtgga gccggaggtg
     4981 agcgaaagct gggacaccgt gcccgcgatc tccgtgtctc cggtggcgtc gcttttgatg
     5041 atggcctgct ggcccacagc cacgcttttg atatcgtcct cgtcaatggt aatggacacc
     5101 ttcaaatcgt cggtgttctg gatgaccgcc aacgccgtgt tggaggtacc gccgcccgcg
     5161 gccgagccaa ccgtcgcgtt cagcgtagtg atggtgccgg acgcacctgc ggtgatggtg
     5221 cagtccgcga tctgctcctc cagctcctcg atctcatcgc tggtgcccgc gttttccaga
     5281 ttttcggccg cggtctccat gttttcctta gcctgccgga gggtttccgt agccgtctcg
     5341 taggtgctcc tggcctgcag ccaagcctgg tccgccgcgg cataggcctg atacagcgcg
     5401 tcgtagccgc tgtttgcctt cgcagtcttg agcgcatcct ccgccgcctg ccgcgcggcg
     5461 gacttcgcgg tgtaatcgct ttgcgccgcc gcggccgtgt tctgcgccgc cgtcaaagcc
     5521 gccgccgcgt tgtcccgggc cgcctgcagc tccgggttgg cgctgtccgc atccagcgcg
     5581 gcctgtgcgg cgtcgcaggc agcctgcgca gcggaaagtg ccgtgttggc gttgttcagc
     5641 gccgcgcccg cctcctgctc cgccgtcaga gccgcgtcgt aggcgctctg atacgacgaa
     5701 acggaattgg ccgcgttcag atattcggtg ttgcgcgccg tttccagcgc cgaagccgca
     5761 ctggcgtagg cgttgtacgc ctcggtctgc ttcgccgccg cgctgttgta gctttccacg
     5821 gccttatcgt aatttttctg cgccgtttcc ctgctttcgg ccagcgcctc ctgccgtttg
     5881 gcaagctgtt cctccaggtc ggaggaatcc agcgtgcaga tgacgtcgcc ctcgcttacc
     5941 gtgtcgccca cctgcacaaa gatctccttg acgggatagg agagcgacgt ggtgacgttg
     6001 gccacgcttc cgctctccac cgtgcccgtg acggtgatgc tctctgacag cgtggttttt
     6061 tccagccggg tggtgcgcac cgcgtcagcc attgcgggca gcgccccgcc ggcagggcgc
     6121 aggaagcgcc agacgccgaa gcccaatgcc gcagccgcca agacggccga caccaggagc
     6181 acacgctttt tggtgaaccg gaacgaccgc agccttttcc acagccgttt gaaccaaagg
     6241 cctgcttttc ccataataaa acccctttct ttgtaccgga tctttacaaa aaaatggtaa
     6301 ataaaaaatg tgaaagggat gtgtggagca gcggaaagaa cggtgaaaac aacgcggaaa
     6361 agccgcgccc caagggaggc gcggcctaag cgcaaaagga gcagagccgc cggtcacggc
     6421 gagggcggcg cgcttttcgc acgcggcagc tttaccgcgc attcacgctc cgcccgcctg
     6481 aaaaagcgcg ccagcgtcgc cgcggccgcg gcagccacct ccagctcggc cgctgcctgg
     6541 gcgcagggca ggccgtacag cgggaacaat gcatccagca ggttccagat ggccataggg
     6601 catgcaaaga cgttttccac gcccccaaaa ggagctttcg cgctggagga cttgctcgag
     6661 aaaataaaaa tgtatatact cagtacaaag aaaaggaaac aggtgagccc gatggtgcgg
     6721 aaataatagg cctaaaaccc aaaaggaggt tctctgcgtg tcaaaaaata agacagccaa
     6781 agagaaaaga ccttggcata tattgatttg gcttatactg tatcttgtta acctgcttgc
     6841 agccttggcc ttccaatatt ttttcattca tacatactcc gcgccattaa cagcggaaag
     6901 tctcgggagc atttcgtatt ttaacgggtg cgaaatattg gacataagcg gtgagggtgt
     6961 tcccgataac agtttgattg gcaaaagttc accggactgg attctttata aaaatcaagc
     7021 tggtgagact catgctgtac ggattgaatg gaatttatat atgccaagat tcaggattga
     7081 aaaaaacagt aatacgctta ttccaaatga agaaagctac accttttcaa tgcgggactt
     7141 tttgggaaaa aacgaagtta ctgttgaaaa tcaagaggct attgttcaat taaagtggtc
     7201 cggattattc aggcagcagg gttatattca gggcatacat atgttaaccg ctttgggatt
     7261 gctgcttctg gaggcgtgga tttatctccg tattcaaaaa aaacaaaacg cataattaca
     7321 gcagttacaa ctgtcaatta aacattttga gtagtaggat ttgttaggag caatcattca
     7381 tcttattact aaatcacaaa ttttggcaca atgaccatcg taaaagcaaa tatgcataag
     7441 catgatacaa aagcaagaga atcggcatca agcgccgcat tactatacct ttgtggcata
     7501 cttcgttgac ggttttggca gtctgtatgg aagtcccttg cgaatctaac ggtaggaaac
     7561 aataatttat cttagtgtgc aacttagctg cttcacaaaa ttgtatatat taaccatcta
     7621 ataaagcacg gcaactcaaa atgagttgcc gtgctttaat tgggtaagcg atagaaacag
     7681 cctcaatgga aatctttagg caggttgttc atattcttct tgtttacccc attgaacgct
     7741 aacgaatatg gcgcagcgat aagtgcaaaa ttcttcaaat aaaagccaga caaaagagcc
     7801 acggtttatt gccgcctttc agtatggcgt gttgcagctt tgttccgtca gagttctgca
     7861 atcatatctc tttcttttgg gcgaaacttt tccgccttat cccaacggat aagctacttg
     7921 caccacagga tatcaccggg gatggcgctt gatgccgttt tatgagcagc tatgcgaaaa
     7981 gacgcttttt gccgtttccc tccggcgggc cgctgcccgc tcacagcacc tcgtatacgt
     8041 ttgcctcata ggggcgcagc acgggcgctt cctcggcata attaccgatt cgctttgcct
     8101 gtgcgccgga gagcggcgcc gggcgcggcg tctggtgggc cgtcaggttg cattccacat
     8161 aaaattttct gccgtccagc acacggaaat agcagaacac atccgcatgc cttttcgtct
     8221 ttaaaaacac gggacggaac gcgccgtata ccagcgccgg gttttcccgc cgcagcgcga
     8281 tcannnnnnn nnnccgcgtt ccatggcatg ggcgcgcggg catgatcccg cgtgccggcc
     8341 aggatgcgct tccacgcttc ctccgacggc attttgccgc acagctcctg gtacaggttg
     8401 cggctctcca cgtcgttgat ctcgtcgatg gatgcaaaat ccacattggc aaggcccagc
     8461 tcctgcccct gatagatgaa cggagtgccc ttgagcgtga gctgaagcgt cgccagcagc
     8521 tttgccagcg gcgcgcgcac agccgggtcc ggattcactt tgctcaccat gcgcgggttg
     8581 tcgtggtttt caaaaaacag gctcatccag cagttgtcac cgtaattctg ctgccagtcc
     8641 aacaggtatt ttttcaggta gcgcaggtcg taccggtaat cgtcaaagcg ggtcttgccc
     8701 gggttctcca gcgcgtcgaa gttgaacacc atgtccagct ccgcgcggct ctccgcggtg
     8761 agcattttgg acatctgcat gcccaggccc gccgtctcgc ccacagtgac ggcgtcgtaa
     8821 cggccaaagc tctcccgccg catctctgcc agatattcgt gcaggcgcgg gccgtaaaaa
     8881 taatgctcga tgccccgcag gccgatggct cccgccaacg cctcgctgcc gtcagccagg
     8941 ccgtccttag agatcaggtt gatgacgtcc atgcggaaac cgtccacgcc cttggccagc
     9001 caccagtcga tcatgtcata gacctcgcgg cgcagcgcgg ggttatccca gttcaggtcc
     9061 atctgctttt tgctgaacag gtgcagcgcc cattcgtccc gttcgggata gtaattccac
     9121 gcggggccgg aaaaaaagct cacccagttg ttcggctctt ttccgtccct gcctttgtgc
     9181 cagatgtaat agtcccggca gggaccgtcg gggtcgcgca gggaactttg gaaccagggg
     9241 tgctcgtcgg aggtgtggtt caccacaagg tccatgataa gcttcatgcc gttcgcatgt
     9301 gcctcagcca gcagggcgtc gaaatcctcc atggtgccga actcggccat gattttccgg
     9361 taatcccgga tgtcatagcc gttgtcgtcg ttgggggaat cgtatacggg cgagagccac
     9421 agcagattta cgcccagttc cttcagatag ggcagctttt cgcggatgcc ccgcagatcg
     9481 cccacgccgt cgccgtttga gtccgcaaag ctgcggggat agatctggta ggccacggcc
     9541 tccttccacc atttgcgggt gatgccgcct gtgggcgcgg gcggcgtctg cgtttcatgg
     9601 ttcagcaggc ccagcaggga atccaccagg ctgtcgtcca gcctgcccgg cgccagcttc
     9661 tgcaaagcgc gcagtgaaag gcggcgcacc acagggttgg cgacggcctt ttccggaagg
     9721 cccgtctgca aaagcagcgt atggatgatg tcgtgcccaa tgggcgtcgc atagatctcg
     9781 ccgatgcggc tgtcccgtgt cagcataaac aaacctcctt cggcgcggcc gtgccgcgtg
     9841 cttccaacaa acgcgcgcct caccgtgcga acgtcatcgc aaggccggtg ctccagcttt
     9901 cgccaggcgc aaggcgcacc gtgtcgggct tctcctccca gatgccgctg gccgtgcgcg
     9961 cgtccggcaa gccgtgccac ggctcgatgc acacatagcg gacagggcct tttgcgctcc
    10021 acatcagcac ataggggtag ccttcgatcc ccacgctcac gcggcggccc gtctcttttt
    10081 ccacaatgga aaggctcccg gccgtcagcc gggaaaagca ggtgctgtcg tggtcgaaca
    10141 tgccgtcatg cagcggaatg tctgtctcac tttcaaaata aacacgggtc gcgcccgtca
    10201 caaggccgtc gtcctccccg gtttcgatga cggtgggcgt ctgcggcgta tcgaaccgca
    10261 gcacgtaatc ctccgcttta tgcgccgcgt cgaacgggca cagaaacgcg ggatggtacc
    10321 cgaaggcgaa gggcatcacc tcgccgccgt cgttctccac acggatgtcg tggtgcaccg
    10381 tcacgccctc cagccggaaa gtggaaaaca gcttgaatgc aaacgggaat ttttccatcg
    10441 tcagcacatt tgcctccagg cacagcgtca cgctgtcccc gctctgttcc accagcgcgt
    10501 gctccatatc ccgggcaaag ccgtgctggc cgccctcata ggacacgccg ttgtgggtga
    10561 acgcgccgcc cttcagccgc ccgcagtagg ggaacagaat aggcgcatgc cggttccaca
    10621 cagctgggtc cgcctgccag agcatctcct cgcccgtttc ctttttcacc acgctgcaca
    10681 gctcagcgcc cctgctctgc acccgcacgc acaaaaaatt attttcaatc gtaaattcca
    10741 taaacgccct ccgttcgctc caagattcgg gtctaaaacc agtatatccc aaacaacggc
    10801 aaaaaggaag agggagcccg ggtgtcagca caggttttca aagaatatcc taagacaaga
    10861 aaggagtgtt tgtatgaact ctgacaaaca aggccgcgcc atgtgcgcat gcggcagcag
    10921 cggtgccgct ccgggtgcgg agcccgccgc cgctgcgccg cagtgcaggt gccgcaccgc
    10981 accccgcgcg ccttcacccg ctcaaaacat caccgccgcc atcgccgcgg cggaccttac
    11041 cggcacaggc tttctgcgcc tgcgcagctt cgccgccagc aacgcgcagc ccgtgcctga
    11101 cgtcatcgtc accatatccc agcgttccgg caccggaatc ccggacaaaa ccgtcaccac
    11161 cggtcccgag gcattgtccg acgacgttgc catcgcctgc ccgccccgct cttactccct
    11221 ggacgaaaac aaccgtaccg tcctgcccta tgccgtggtg gacgtgaccg ccgagcatga
    11281 gggctatgac cccgtgcatg tggcgggtgt gcaggtattc gacggtgagg taagcctgct
    11341 ggaactgctc atgatccctg tggaggagga cgaaacggcc gcctacaccg tgcgcagtct
    11401 ccctgaggat ttcgatatcc ccacgcacaa gctgttcgag ggcggcgaat cctccggccc
    11461 caccccgctg tctgcctgcc gcccgcgcat tctggaccag cccatcatcc ccgaaaagat
    11521 cacggtgcac ctgggccgcc cctcggccaa tgtgcagaac gtgacggtat cgttccgcga
    11581 ttacatcaaa aacgtcgcct ccagcgaggt atatcccacc tggccgg
//
LOCUS       DAWBSB010000051        11609 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1311_length_11609_cov_3.086723, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000051 DAWBSB010000000
VERSION     DAWBSB010000051.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 11609)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11609)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11609
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1311_length_11609_cov_3.086723"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    7468..7477
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8140..8149
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2028 a         3887 c         3398 g         2276 t
ORIGIN      
        1 ggacgtactg tcggagtcca tcaacaatgc catccgcctg ctgctgggct ggtttttcgt
       61 aacggcggac tatctgccgc cggtcaccat cgtgctgggc tactggctgg gcggcgcgtt
      121 tctcatggcc atcaagcgct atgccgaata ccgtatgatc ggtgacaaaa gcaccgccgc
      181 gctgtaccgc aaatccttcg gccagtacac cgaacagtct ctcctgatct ccgcgttctt
      241 ctacgccatg tgctcgctgt ttctgtgcgg tatcttcatc atcaaataca agatcgagct
      301 actgctggtg gtccccttcc tgtgcgggct gttctgtttt tactttcatc ttgccttcaa
      361 aaaggattcc gccgtgcaga agccggagaa gctttaccgg gagaaagggc tgctgatgta
      421 catgatcata ctggccgccg tattcattct ggcgctgttc atcaagatcc cggcgctgga
      481 ggtctttctc gactccaaga tcatcggctt gtaatggtgc gccgccatga acgtatacga
      541 ttttgacgac acgatctttc gcggcgacag cacccgcgct ttctgggcct actgcctgaa
      601 gcgcgcgcca gggctggcac gctttctgcc ccggcagtgc gccgccgctg tgcgctatgc
      661 gctgggccgc acggctaagg aagcgttcaa ggaagcgttt ttttcctttt tgcagggcgt
      721 gccggacgcg gacgcctggg cggaggattt ctggcgcacg catgaacgcc ggttccgccc
      781 gtggtatctg gcccagaagc agccggacga cctggtcgtt tctgcctcac cggaatttct
      841 gctggcgccc gcctgccgca ggctgggtat ccgcccgccc atcgcctccc gtgtagatgc
      901 gcgcaccggg cgttacacgg gcctcaactg caagggagcg gaaaaggtgt cgcgtttccg
      961 ggagcaatat ccggatgcgg agatcgggca gttttattcc gattcccgct cagacgcgcc
     1021 gttggcgcac ctggcacgcc gcgcctttct ggtccgggga gattcttaca cctcatggga
     1081 ggtgtaaggc tccgttcccg cgcggctgcg gcagatattt tcagcacaac aaaagcgcgc
     1141 cctgagcagg ccaacaggcc gattcagggc gcgctttttc aatttgacgt ttccgccagc
     1201 cgcgcagccg gtcaatctct gccgcgggaa gcgcccatgg ctgtgcggcc ggcgggcacg
     1261 ccttccgcgc ggcctccctg cactacgcgc agcgcgggcg caggctcccg cgtcatggcc
     1321 gggccgtgta cgggccgcgg ctgctgcacg cggggcataa ggccctgctg cgccgcgagc
     1381 atggcagcac ggcggcgtgc cagcctgcgc tttttgcgct gcgccttaac gtacgccaca
     1441 taagcgatgc gcccggccac cagagcggcc agtaaaagaa agatacaaaa cagcacgccc
     1501 agcaccttga gcgcgacctc ccaccaggcg ggcctgtcca caaattcctt cgcattggcg
     1561 tccaaaagcg cggacactgc gctctcgtcg atgcccaacg tataaacgcc gatctcgcgg
     1621 ttcatacagc tcccggccga aaggccctcc ctcaggctga ccgtcattgc tgcggtgaac
     1681 ggctcgtcca ccatgtagcg ctccggcgta tcaaacgtga cctgcacgtc atccgcagcc
     1741 agcgcactgt ggagcaggaa cgtgtagccc tcctccgaaa gggccagcgg cgcgctgccc
     1801 accgagggcg ctccttcggc gtaatacacc ggcacctgcg gggacgtaat atccgaagcc
     1861 gccagcgtga cgggcgtgaa atgatcgaag caataatcca gcaatgcgat gctgtcggaa
     1921 aacttgtcat acttttttgc gcttcccatc accacgcaga tcagacttct gccgctgcgg
     1981 gtgacggttt ccaccatcgt gtagcccgcc tcctccgtcc agccgctctt accgccggtg
     2041 gtgccctcat actggtattt gttcgttacc agcatgcagt tatcggtgcc aaacagacgc
     2101 tcctgctcct gcttgttggt gggctgcatg gtataggacg ttgcgccgaa caactcggaa
     2161 aagcccggca ccgtcatcgc ccactgtgtc aggcgggcca tgtcgtaggg tgtggtgtag
     2221 tggtcggcgc tgtgcaggcc gttggcattt acaaaatggg tgcccgccag gcccagctcc
     2281 gcggctttgg cgttcatcaa ctccacaaac gcctccatgc tgccggcggt gtactctgcc
     2341 agcacattgg ctgcgtcgtt ggcactggcc aggatcgtgg catggaacag atcgttgaga
     2401 gacacgacct ctcccggctg cagcgcgaca tgggacgagc ccgcctccag cgaatagacc
     2461 gcgtccgggc tgaccgtgac ctgctgcgtc atgtcgccgc cgcatttttc catagccagc
     2521 gccagcgtca taatcttcgt gatactggct ggataattcc gctcggcgcc gcccttctgt
     2581 acgagcacct gccccgtctg tgcatccatg acaaggtatg cgtgcgccgt cacctccggc
     2641 ggcacggtgc tgctttcggc gtacaccggc caagccagac ccgccgccag cgccagcgtc
     2701 aggatcatcc ccagcagttt cttcactcgc cgcactcctc acctctgctt tcagcgcttc
     2761 caccgaaaac aagttaaagt tatcaatgat attatacaat ttgtatatca caagtgcaag
     2821 ccttccgccg cttatttcca gtgttttcac attttcggcg gctcatgcct gcccggccag
     2881 cgttttcgcc agcgccagca gcgcttccgg gaagttgatc tgcggcgcgg cgatgaccgc
     2941 gtccagcagg ccccgcagga cccatgcctg ccgtccgccc cgaatgtgcg ccgccgtcag
     3001 ctccgcactg gtgacggcga gctgggcggg aagataaggc tcacgggaga ggcgcagcgc
     3061 cgcgtacagt gcgggctggc tgttccagcg cgggtccgcc agagccagcg tgcgcgcggc
     3121 agcgtcatag tccagcgcct ccggcaggcg gctcagcaca cgcttcagct cctgccggtc
     3181 cgcgggcggt gttttgcggg cagcaagcac gtccagagca gccatcagct ccggcagagc
     3241 ggcgtccagt tccagcgcgg cgcacaggga tgcgtcccgc acggcggatg tcccgcaccg
     3301 ccgcagatac agccaccacc gcgccatagg cacggcgggc gcctccgcca ggccatgcag
     3361 acagggcgcg cgctgcggca gcccgaaaga tgcatacgcc cctgcggcgc acaaaacgcc
     3421 cagtgcctcg ggtgcgccgg agagcaaaag ccgctgtgcc gctgcacccg ccgcgcgccc
     3481 ggacaggctc cgcacgcccg gcgcagcccc ggccgcagcc agcagcgtgt ccgccttcag
     3541 ctccagtccc tgctccgccg ccagcacgaa cagctccagc acccgccgcg catccgctgc
     3601 atagtaggcc tccgggtcgt ccgccgtgca gcagagcaca ccgtcccata tcatatgcgc
     3661 gccttcaaac gctacatcca tccgtatccg cgcccctttg cttccggttt tgttttttcc
     3721 agtataccag aaaatgccct ttccggccaa atttacggga aattgtttat tttaccatca
     3781 aatcaatata taatgttaaa agaacaaggc gtatttttac gaggtgatag atatgaacgc
     3841 attcgacagc aaactgctgg acggcaaaac caagctgcac tttgtgggca ttggcggcag
     3901 cggcatgttc ccgctggtgc agatcctgca tgaagtgggc tatgagatca caggctcgga
     3961 cgtgaacgag ggcgatatca tcgaccgcga gcgcaagctg ggcatcccga tctccatggg
     4021 acacaaggcc gaaaacgtgc acggagccga gctggtggtg tataccgccg ccatcatgag
     4081 gggcaacccg gagatcgaag aggcccaccg gctgggtatc cccaccgtgg agcgcagcat
     4141 ccttctgggt tatgtggcca acacctaccc ccatcccatc tgcatcgcgg gcacacacgg
     4201 aaaaaccacc accacggcta tgacaacgca gatcatggtg atggctcacg cggaccccgc
     4261 cgccgtcatc ggcggcaagc tgccgctcat cgacgcctac ggccgctacg gcacagggcg
     4321 cgagatcgtg gtggaggcat gcgaatacag caacaccttc ctgcatctgg tgccctatat
     4381 ctcggtgctc ctgaacgtgg acgcggacca tctggaattc ttcaagacca tggagaacct
     4441 ggaggaatcc ttccgcaaat tcgcactgct caccacgcac accgtcattg ccaacctgga
     4501 cgacgccaat accgttaagg tcatacacaa tctggaccgc cacatccgca ccatcggcat
     4561 cgacacgccc gcggattacc gcgccgtgca tgtgcgcgaa taccgcccat cgttcttctg
     4621 cttcgacgtg caggtactgg gtgtgcccgc agtgcacatt gaactttcgg tgcccggacg
     4681 ccacaatgtg tacaatgcgc tggccgcttt cgcatgcgcc acgatgtccg gctgcacggc
     4741 cgacgagtgc gcccggggca tcaacgcatt ccggggcgcc ggccgccgct ttgagtttct
     4801 gggagaggta aacggcgtga ccatcgccga cgactacgcc catcacccca ccgagttgga
     4861 ggccacgctg cgcgccgcca aggaaatgga ctacaaacgc gtgtgggccg tgttccagcc
     4921 cttcacctac acccgcacgc agatgctgct ggacgatttt gcccgggtgc tgcagatcgc
     4981 ggacaaagtg gtgatgacgg agatcatggg cagccgggaa cgcgcggagg attacgccgt
     5041 ccgaacgaag gaccttgccg ccaagatccc cggcagcgtg tggtacgaca cctttgacga
     5101 ggtcgtggac catgtgatga aaaacgcccg gccgggcgac cttgtcatca cgctgggctg
     5161 cggcgatatc tataaagccg caaaaaagat ggtcgcctgg gaagcgtaag gcgcaccctg
     5221 cgaaaagccc ggtccccgca gcggggccgg gctttgcagt tttgcacacg gaaaagcggc
     5281 gcccggcatg cagcatgcca ggcgccgctt tgttctgtct gaaaggtcca gggcaatacc
     5341 gcgcaattct gagtattgaa tcgcgccggt atattttttg gcagtttaaa caaaaaacgc
     5401 cgcaaatact ttgtgtattc gcgaggcttt ttgtgacaac tgccggaaaa tatgcaagcg
     5461 gggcgacact taaggcgtca gccatgaatt gtgcggtgct gcctttaatc cctgggcacg
     5521 gctttgtaaa acccgtccag aatcgtctcg aacgccatgc cgtggctggc cttcaccaaa
     5581 accgcgtcgc ccgggcggca gtacgccgta aggcgggcca gcgcttcgtc cttgtccccg
     5641 caatgcaccg cgttcacgcc cagtgcggcg gcgctgtcgc ggccggcccg catggccccg
     5701 cccacggtca gcagcaggtc cacaccgcag cgcgccgcca gcgcgcccac ctcggcgtga
     5761 gcctgccggg tgatggcgcc cagctcgaac atatcgccca gcaccgcgac cctccgtccc
     5821 cggacaggat attccttcag cgtcagcagc gcggcgcgca tgctgtcggg gttcgcgttg
     5881 tagcagtcct cgatgaccgt cacgccgccg tgctccacca catgctggcg gtggcccgtc
     5941 gtttgataat tcgccagcgc cgcggccgca gcggccggtg caaggcccag gcgcgtggcc
     6001 agcgcataag ccgaaagcgc gttgcgcaca ttgtgcaggc ccatggccgg gatgtacgcg
     6061 ggcaggcttc cgtgcgcctt gtccgcgatg gtgaaccgcg tgccctccgc cccggaaacg
     6121 atatccagcg cccgcacgtc cgcgtccggg ttttcaacgg cgaacagcac cttctcaagg
     6181 tccggccgga cggccgcgcc gggcagataa tcgtcgtcgc cgttcaccac cagcggcgcg
     6241 ccctgcggca gcccctcgca gatctccagc tttgccctga ggatattttc ccgcgtgccc
     6301 agttgctcca gatgggagcg cccgatgcag gtgatgacag cgcccgcagg ctttgccgcc
     6361 agcgtcagct tccgaatctc gcccaggccc tgcatgccca tctccaccac ggcgtactcc
     6421 acgcttttgt ccaggcggaa cagcgtgttc ggcatgccga tctcgttgtt ctggttgccc
     6481 tcggtcctga gcgttttgcc gaaagccgaa aacaccgcgt aacagaactc ttttgtcgtc
     6541 gttttgccca cgctgcccgt aacgcccgcc agcagcgggc tgtacagcgc gcggtagttc
     6601 gcgcccagcc ggatcatcgc atccagcgcg tccggcgcct gcacgcattt ctccggcggc
     6661 acgcccggca ccgggcgctg caccacaacg cccgccgctc cggcctccag cgcccggagc
     6721 gcgtaatcgt ggccgtccac gcgctcgccc cggatgcaca cgaacaggct ccccggcccg
     6781 gcctcccggc tgtccgtcac caccgacgcg atctccgggt cccctgccag ggcgcccagc
     6841 ccccgcagca gcacactcga caaaacaggc tgcatataca atcctccatg ctctgaatac
     6901 tcgccgggcg gcgtcaggct ttgtccagcc ctttgcgctc cagccactcg cggacgatcc
     6961 tgtgttcgtc cagataaagg gtcaccccgt cgatgacctg gtaatcctcg tggccctttc
     7021 cgcacagcgc cagcacatcg cccgggcgca gcatatccag cgcccggttc acagccgtgc
     7081 gccgctccac ctccacgatg tacggcttgc ccgaagcggc cagcaccggc tcggcgtcct
     7141 ttattatagc acaaggatct tccttgcggg gattatccga cgttaaaact gcaaaatcag
     7201 catatttcac cacggcttcg ctcatcagcg ggcgtttttt tgcatcgcgc tcgcccgcgc
     7261 agccatacag caccaccagc cgcccctcca cgaaaggcgc aacgcccgcg agcacctttt
     7321 caatggcgtc gcccgtgtgg gcatagtcgc acaggatggt gaacgcgccg tgatacagca
     7381 cctcgcaccg gccgcgcacc ccgcgggagg ccgaaagcgc ggcggcggcg tcccgcgcgg
     7441 ggatgcccag cgcaatggcc gcggccgnnn nnnnnnnatc tgatcaaaac caacgccacg
     7501 atttccggtg cggagggcgg ctgtcaggcg gagatcggca cggcctgcgc gatggccgcg
     7561 gccgccgccg ccagcgcgtt gtgcaccgag taatccccgg gcatggggaa atcgatgcgc
     7621 tggatgaagc cgcggcccac catctcgaac ttcacgcccg aggccgagag ccggatgttg
     7681 cgggcggtgt aatccgccgc gtcgtcgcct gcggaaaagg tgatgcgctc cggcccctgc
     7741 acctcgtcca gcaggcggcg gccgtagctg tcgtccaggt tcaccaccat gtgcttcacc
     7801 tggctgaaca ggcttttctt tgcggcgaaa tagttttcaa aggtcttgtg ataatccaga
     7861 tgatcctgcg tcaggttcgt gaacacgccc acctcaaagg taaggcccca cagccgcagc
     7921 tggtccagcg cctggctgct ggcttccatt accgcgaacg tgcagcccgc cagcaccatg
     7981 cggctgaaca gcgcgttcag gtcccacggc tcgggcgtgg tatattttgc gggcacgtcc
     8041 atggcgtcga tctcggtgtg gatggtgcca atgagccccg ctttgcagcc cgcctgctcc
     8101 agcgtctgct tgatgagcgt ggtgacggtg gtcttgccgn nnnnnnnnnt ttttccgttg
     8161 gtgcccgtga cggccaccag ccgcagcttt tcggcgggac ggccgaaaaa attctggcac
     8221 agctgcgcgt acgccttgcg gccggaggcc acctgcacct cgccgggcag gcccagcgga
     8281 cgctcggtga cgatgcacac ggcgcccgcc tccagcgcct tgcgcgcgtg gtcgtgcccg
     8341 tcggtcgtca tgcccctggc gcacacgaac accgcgcccg ggcaggcccg gcgggaatcc
     8401 tgcgtgacga gcaacgcctc gccctgcggc agcgtgccgg tatacggcac gccgctgaac
     8461 aatacctgta actccatgga tgcactcccc tttcgtgccg cgccgcgcgg cccgccgccc
     8521 tgacgcggcc tgttaatcca gaacggtggt atcgtggaac gtcacctcga tgacggaccc
     8581 gatctccacc tgcgtatcgg ccgcgatgtc ctgctctgtg accacgatgg tcttgccgcc
     8641 gcccgtcacg cccttttcgc tgacgttcag gcccagcgat ttcagcgtgg cgcacgccgc
     8701 gtcgtagctc atatccgtca ggttcggcac cgtgacaagg tcgccgccgc tctcggaacg
     8761 gtcctccgaa tacaggatga tcgtggactg gcgcggcacg atggtgcccg ccgccgggta
     8821 ctggtacagg acggtgtcgc ctgtggcgcg ctcgatgtcc acattgaagc cggattcgcg
     8881 cagcttgccg gcggcggcgt tcacactgga gcctgccacg ttgggcaccg ccgtctccac
     8941 ctttgccagc tccgcctcgg tgtactcctt gggcaccccc agcgtctgga gcgcctcgtc
     9001 cagcacactt tgcaccacgg gcgcggccag acggccgccg taataggtcc cctgttcgtc
     9061 gtgcggcgtg tccagcatca ccagcaccgc gatctgcggg tcgtccccgg gcgcgaagcc
     9121 ccaataagag gaataatagg tctgctcttc gtccttcagc gcctgcttgc gctggctggt
     9181 gccggatttg ccgcccgcct tatagccggc cacgtagccc gtcttgtttc cgccgatcag
     9241 cgtcttgcca tccgcggccg tcatatttac gcttctcgtc agcagctcgc gcatggtggc
     9301 gcttgtctcg gccgagagca cctgccgctt cacttccggc tccacgtttt caacgatatt
     9361 cccgtccgcg tccagcacct gcttcaccac gtgcggctgt accagatatc cgccgttgat
     9421 gacggcgcag gccgctgtga tcatctggat gggcgtggtg gacgtcgtct ggccgaaggc
     9481 ggaggaggcc agctccacgg gccccattct ttccagcggg acgtactgtg tcgttccctc
     9541 gtcgtccatg tcgacgcccg ttttctcgta gaagccgaag gcctgcatat aattgcagaa
     9601 cgtctccgcc cccatcatct gccccagcgt gacatagtag gggttgcacg agccgtccag
     9661 gccgccgaag aagtccagcg tgccgtggtt ggtgtgcgcg caccgcatgg tgcggcccgc
     9721 cacggtgatg gagcccttgc aggtgtacga ggtgttcagg ttcgccgcgc cggagtccag
     9781 cgcggccgaa gccgtgatga ccttgaacac cgagccgggg tagtacaggt cggccaccgc
     9841 cttgttgcgc cattgcgccc actgcgcggt atatttcgcg tccgcccgtt cgttttcatc
     9901 cgcaatggcc gcgatctgtg ccgccagcgc ctcgtccgca atggtgtaag ggtcctgcag
     9961 gttgtagtcc ggcatcgtcg ccatggccag aatcgcgccc gtgttcacgt ccatcacgat
    10021 gcccaccgcg cggttttcca catgatactg gtccacggcg ttctgcaggt atttttccaa
    10081 tgtgctctgg atgctggcgt tcagcgtcag caccaggccg ttgccgtcct cggcgtcata
    10141 ggccgcctcg taggtgccgt ttggcaaatc gtacccccag gcgttcttca tgcccaccac
    10201 gcgcccggcg gtgcccttga gcgtatcgtt gtaatagctc tcgatgccca gcacgccgtc
    10261 gccgtccgcg ttcacaaagc cgatgatcgt ggaggccagc gtctggtagg gataataacg
    10321 cttcgcgtcc tgcaccaggc tgatgcccgc gacaggagta tcttttccct tgtccgatgc
    10381 gttatactcg gtgatccagg ccgtgatggc gtccgccacc ggcttttcga tctgccgctt
    10441 gatgagctga tagttgcttt cgctcttctg aagcttttcc aaaatgctct gggcgtccag
    10501 ctccagcagc tcggccagct ttacggcaac gtcgttgata ttcgtgccgc ttttggccat
    10561 gtcccggggc gaggcgacca ccgtccacac cttgttgctg cgggccagca ccttcatatc
    10621 ggaatcgtag ataacgccgc ggttgggtgt gatggtctcg tccgcaagct gctggttttc
    10681 ggcgcgggcg gccagttcgt cgtgctgcac gatttgaagc tggtacaggt tgtatacaac
    10741 ggccccaaag cccaccagca cgaacagcgc ggccagcacg atggcgcgcg tgcgcatgct
    10801 gcgggacacc gcgctcttgg gcgccttcgc cgtgtcctgc ggcggtacgt tctttcgatt
    10861 gtgttccggc tgttttttca tctctgcctc ccgggatatt gctgttgtca aagggcgcgg
    10921 gccagccgcg caaacggtcg aacgcgggca taaaaagaat tatcaccggc ttttcccgca
    10981 cgaacggctc ggcagaggaa gaaaggcggt gataatactt attgattcag ggcgtgaggt
    11041 attccataaa gctggccacc gtctgcgata tctcgcccgc caggcgggcc agcccggtgt
    11101 ccgagcgctc caccttgttc tcctgcccgg tggtcacata cgtcacctgt gagcggtcca
    11161 tctttaccat tttcagcgta ttcgttgcat attcctgcac cgccgaaagg tttgttttca
    11221 tttccatctc attggacagg tatgtatatt cgctctgaag gtcagccagg gtgtcctcct
    11281 gcgtgcggat ctgggccgaa agctcagtgg actgcacctg cgagtacagc acgctgcaaa
    11341 ccagcgccag aaacacggaa gcgaacgcca cggctttgat ccattttaaa ttgtaccgcg
    11401 ccagcccccg tttgggcttt ggctgcccgg ccacgcgcag acgcggcttt tgctcccgcg
    11461 gagcgaaccg ctcaaggtcg tacgctgcat tcgtctgcat ggcgtcctct ctcctttctt
    11521 ttttgtttca aggcaatacc gtacaattct aagcgtcgaa tcgcgccggc cggttttttg
    11581 gcaggttaaa caaaaagcgc agcgaatac
//
LOCUS       DAWBSB010000052        11566 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1314_length_11566_cov_3.106507, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000052 DAWBSB010000000
VERSION     DAWBSB010000052.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 11566)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11566)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11566
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1314_length_11566_cov_3.106507"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    364..373
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2336 a         3639 c         3328 g         2253 t
ORIGIN      
        1 acatctgcaa cgccctgcgc accagccagc tgttcaacac gcctaatatg agccgttcgg
       61 cctatgtggc gaaggtgaaa accgaaaact gcgttgcctg cggccgctgt gtggaatact
      121 gccccgcggg cgccgtcaag ctggggcaaa agctgtgcac ccaaaacggc ccggtggaat
      181 atccccggca ggagctgccc gacgccgtga agtggggccc ggaaaaatgg gacgtcgatt
      241 accgcgacaa caaccgcatc aattgctacg acgccggcac ggccccctgc aaaacggcct
      301 gccccgcgca catccccgtg cagggctacc tgaaaatggc cgcgcaaggc cgctacaccg
      361 acgnnnnnnn nnncggcacc gtggatcagg ccgttgccat cgacgaggta aagaaattta
      421 ttgcccagca ggacctgaac gccgccacgc ggtatgtgcc gccggtggtc gcacccacga
      481 accggggcgg cttcgcggaa aagatcgccg tcatcggcgc cggcccggcc ggcctctcct
      541 gtgcatatta tctggccgag cgcggctata agcccacggt atttgagaaa aacgagcgtc
      601 ccggcggcat gctggtatac ggcatcccct cctacaagct ggaaaaggac gtcgtggacg
      661 cggagatcga ggtgctgcgc cagatgggcg tggacatccg ctgcggcgtg gaagtggggc
      721 gcgacataac gctggacacg ctgcgccggg agggctataa ggctttctac gtggccattg
      781 gctgccagga cggccgcagc gcgggcattc ccggcgagga caccgcgggc gtgatgaccg
      841 ccgtggactt cttgcacgcc gtcgcggaaa acgaaacgta ccccgtggag ggccgggccg
      901 ttgtcatcgg cggcgggaac gtggccatcg acgtggcccg caccagcgtc cgctgcggcg
      961 ctgcagaagt ttccatgtac tgcctggagc cgcgggaaag catgcccgcc tccgccgagg
     1021 aagcagcaga ggccgaagat gagggcgtgg ccatccgctg cggctggggc ccgaaggaga
     1081 ttcttgcgga agccggccgg gtgaccggca tcgttctgaa acgatgcgtt tccgtgtttg
     1141 atgaaaacgg ccgcttctgc cccagatatg acgaggccga taccattacc gttccctgtg
     1201 aacatgtgtt tctgagcatt ggccagagca tccggtgggg cagcctgctg gacggcgcaa
     1261 aagtagagct gggacgcggc ggcgccgccg tggcggaccc gctcacctac caaacggcgc
     1321 agccggacat atttgtaggc ggcgatgtgt acaccggccc caaattcgcc atcgacgcca
     1381 ttgccgccgg caagcagggc gcggaatcgc tgcaccgctt tgtacacgaa aacgccagcc
     1441 tgaccatcgg ccgtaaccgc cgccagttca aggagctgga caaggaaagc cttgcgctgg
     1501 agagctacga caacggtgca cgccagagcg cgggcatgga cgagcgcatc gactaccgcc
     1561 gttccttccg ggacgcgcac aagaccttta ccgaggagca ggtgaaggcc gaaaccgcgc
     1621 gctgcctggg ctgcggcgct tcggtggtgg accccaacaa gtgcatcggc tgcgggcttt
     1681 gcaccaccaa gtgcgagttc gacgccatcc atctgaaccg ggagcttccc gaatgcagca
     1741 ggatggtgcg ttcggaggat aaattcaagg ctattctgcc ttatatgatc aagcgtgaaa
     1801 tgaagatccg cttcggcaaa aaggacggct gagacgatgc atgagcttgg gatcgtattc
     1861 catatcatcg acagcatcga ggaggttggg cggcagaaca agctgcgccg cgtctccacg
     1921 gtgacgctgg agctgggtga ggtctccggc gtggtgcata cttatctgga ggactgctgg
     1981 cactgggcgg cggccagatc gcaattgctg aacggctcgc gcctcgccat cgaggcgctg
     2041 cccgccgtta ccctgtgcga ggactgcggc agcacctacg ccacggtgga gcacggcaaa
     2101 acgtgccctt tctgccaaag cccgcgcacg catctcgtca gcgggaatga gatcaatatc
     2161 aaggagatcg aggcgtgcta gggacgcgcc tttgaccggg tacacggcgg cgctgccgcc
     2221 aaaaacaagg gaggaaccgt atcatgttat ttcagatcta cggcgaaaac gccggcttcc
     2281 agcttttggg ctgggctctc gtctttgtgg gcctcatcgc ggccaacgaa ctggcccgcc
     2341 gctccaaaaa gggcggtatc ttcttcttcc tggcgcttcc cgccgcactg acggtatatt
     2401 ttatcgccat ttacgcgggc gcggccatgg gcgcggagtg ggcgctgaac aaccccacct
     2461 acacgcatat gaacagctgg ttccactacg ccaagctgta cgccgccacc gcgggctgca
     2521 tcgggtttat gatgctgaag tataaatgga gcataggaaa gacgcagtgg ttcaaggttt
     2581 ttcctttcgt catcgtcgcc atcaatatcc tcatcgccgt gggcagcgat tttgaatccg
     2641 gcatccgcgg cgcaatggcc ctggctgaga ccggagaccg ctggtggctc tccagcgaga
     2701 acgtttggct gtacggcggc tggtggaact gggtaaacgg catcgccggt atcctgaaca
     2761 tcctctgcat gacgggctgg tggggcatct actcttccaa aaagcacgac gacatgctct
     2821 ggccggacat gacctggctg ttcatcatcg cctatgacct gtggaacttc gagtatacct
     2881 acaacaacct gcccacccac gcgtggtact gcggcttggc gctgcttctg gcgcctacgt
     2941 tcgccaacgc gctgtggaac aagggcggat ggattcagaa ccgcgccaac acactggcgc
     3001 tgtggtgcat gttcgcccag gtgttcccgt tgttccagga cgccagcgtg ttcaccacca
     3061 tccctgtgct gtatgcggac ggctttatgg acgccgcggt gcgcccctct gccgccgacc
     3121 ccacgatgca gggcgtggtc gccattctgg cgctggcggc gaacgtcatc gtactggcgg
     3181 tcatcatcaa gcgttccctc gcacaaaaga aaaaccccta tacgggcgag atcttcaccg
     3241 atacgcggga tttccggctt gccatggcaa gagccgaggg caaataacca atacggcgca
     3301 ccccgcccaa aatatacggg gcctgtttgc aggccccgtt tttcatggaa aggacgacag
     3361 catgaacaag cgtgagatca tcgcatattt tgacgcccgc gcgccggaat gggatgcgcg
     3421 catggtgcgc agcgacagca agatcgcctt catcctggac gcggcgggcg tgcggccagg
     3481 cgtttcggtg ctggacgtag cctgcggcac cggtgttttg tttggcgatt acctggcccg
     3541 ggacgcggcg cgggtgacag gggtggacat ctcgcctgaa atggcacgca tcgccgtttc
     3601 caagctgcgc gacccccgcg tggaggtact gtgcggcgac gtggaggagc ttgcccttcc
     3661 gcgcaggtac gactgctgtg tggtatacaa tgcgttcccc catttcgccc accccgcccg
     3721 gctgctggca cgcttatccg cctgcctctc ccccggcggg cggctcactg tggcccatgg
     3781 catgagcctt gcggcgctgc accgccacca cgcgggcgcg gcccgcggcg tgagccggcc
     3841 catgctggag ccggaacgcc tggccgcgct gatgccgccg tgccttgcgg tggacgtcgc
     3901 ggtctcggat acggaaaaat acgtcgtctc ggcgcggctg cggtgagcgg cgtacacaaa
     3961 ggtttttcaa aggcgccggc tcccgagccc gcgccttttt ctttccccgc gcgcatgcac
     4021 cttgcaccgc gcttctccat acaatgatat gagagcgggc ggcacttgcc ccgctttgaa
     4081 atgcggcgcc gggcctgggg ttctgccggg ctgtcccggc attttgtctt ccccttccct
     4141 ccggcgattc ggccgggccc gcctgccgcg caatgaagga gaacgcatta tggaacaagg
     4201 tactcttttc gtaaagcttg aaagcgcccg cgaagctctg cccgtgcagg gccgtgtggt
     4261 catttcgcag acagtgaacg gcgtccgcgt gctggagatc gagctggtaa cggacgagga
     4321 cggtctgaca cagcccgtca cgctggacgc accggaccga tccatctcgc tggacccggc
     4381 cagcacccag caagcctggg ccgcctatga cgtggaggca tatgccgacg gctactccga
     4441 cattcttctg gagggcgtac aggtatattc cggcgtggag gcgtacgcgc ccatcagcat
     4501 gcttcctatt ttttcctccc aggcggtttc gcccacagcc gtcacggcgg acaccggttc
     4561 cacctcccgc aaggcccagc aggacatcct gcacaccacc atccccgcgc ccgccatccg
     4621 cggcggctcc gccagcggcc ccgcgccgct ctccacctgt gccacgcagc ctctggtgct
     4681 gcaaagcgta ttcatccccc agtacatcac cgtgcatctg ggcaagccgc agaacagcgc
     4741 cgccaatgag acggtcagtt tcccctatta catcaaaaac gtctgcagca gcgagatcta
     4801 ccccacctgg cccgaaaatg ccatccgtgc caacatctac gcccagatct ctctggcgct
     4861 gaaccgcgtt tacaccgaat ggtatcccag taaaggctac aacttcaaca ttaccaactc
     4921 cacccagtac gaccagtatt acgtttccgg ccgtaacatc tttacaaaca tcagccgcat
     4981 tgtagatgac atcttcaaca cttacctgcg ccgcgtgggt gattttgccc cctattatgc
     5041 cgaatactgc aacggcacca ccgtcacctg caaggggatg agccagtggg gcaccgtaag
     5101 cctggcggaa agcggccttt cccccatcca gatcctgcgc cggtactacg gcaacaattt
     5161 tgaactggtg accaccaacg acatccgcgc cattcccagc tcgtatcccg gcacgccgct
     5221 gcgtctgggc agtacgggca acaacgtgag catcatccag cggcagctca accgcatcgc
     5281 caaaaactat ccggccatcg gccagcagtc ggtggacggc gtattcggcg caacgacaca
     5341 gtcggccgtg cgcacgttcc agaagatatt caatctgacg caggacggcg tcgtcggaaa
     5401 gtccacctgg tataaaatca gctatatcta tgtagcggtg aaaaagctgg cggagctggg
     5461 cagcgaatcc gaaccgtatc ccggcggaag cggcgacgcg cccggcagcg gcacaggttc
     5521 tatccagcag ggcgccgtgg gcgcacaggt ggcggccgtg cagtattacc tgagctacct
     5581 tgcgcaaacg ttctatccca caatccccaa tatcgtaccc gacggtattt tcggcgcaca
     5641 gacgcgcagc gccgtcatcg cgttccagaa caccttcggc ctgacggcgg acggcattgt
     5701 tggtccggcc acctgggccg cgctggaaaa gcagtttgaa accgcctatg acgacaacaa
     5761 ccccggcagt tatttcggcg cgtatcccgg cgtcgtgctg cgggagggca accgcggcct
     5821 gcgcgttcag cagatgcagt tttccctgct gtacctgcac tactcctatt cctccatccc
     5881 caagatcaac gcggacggca tcttcgggcc cgccaccacc gcggccgtac gcgcgttcca
     5941 ggcaaaattc gggcttacac aggacggcgt ggtagggctt gccacctgga cagagctgta
     6001 ctccatttac agccagaaaa acgcactggt gctctcggat gaacaagtgc ctgcttatcc
     6061 gggcttcgtg ctgcgcaggg gcagccaggg ccaggccgtg ctgggcgtgc agacttacct
     6121 gaacgtcatt tcgcggaaat acgcctccgt cacgccgctg acgcgggacg gcaattttga
     6181 cagccggacg gaggtatccg tgcgggcgtt ccagcggcag ttcaaactca ccattaccgg
     6241 ctgggtggat gagccgacct ggaaccgtat ttacagcgag tacaaaaaca cacttctttc
     6301 cgagcacctg gaatgccgct ttatcgacgt gccctatccg ggcgagcctg tcgaggtggg
     6361 ccagaccaat atctttgtgc gcacggcgct gtattactac aatctgatcg ccgcattcga
     6421 tcttctgctg gagcccacgg acatcaccga cactttcact ttagaggact ccctcgccat
     6481 cgaggaattc caacgcacgc gcggattgac tgttacgggc gtcatcgacg aggatacctg
     6541 gacgctgctt tattaccaat acgtggccgc ttatcaggac gttttcccca actgcgcggc
     6601 tcagggctcc gtcccgcagc ctttccagac gctggtgccc ggcagccgcg gcgtcgccgt
     6661 ggagcagctg cagatctgga tcaacgctct gagtgcctat tactgcgacc tggtgcccca
     6721 ggacgtgacg ggcatctatg acgagacaac ggaatcgaac gttttcatcc tgcagacgct
     6781 gctggacctt ccgctcaccg gcgaagtgga ccagaccacc tggaacctca tccaggaggc
     6841 ttatgaagaa gcgctggccg aaaacaacaa tggggaggaa gcctacggcg acctgctgga
     6901 tgtcacctac atcaaccgca ccatttccat gacggaaaca gtcctatgcg cgggcaactg
     6961 cggcacgccg ctggactgac gccatacagc ctccatgtcc gcgcgggcat ggcagcacag
     7021 gaaaaaggct aaaaacccgt ttgcccgccc ggacatgagc cgggcgggca agcgcctcta
     7081 ccgcacaaaa gggagaagtg agggaaacca ataaacgcct gcgcagcaca aagcgtttgg
     7141 tttccctcac ctccgaggct ggcggctttg tcgccggcct ctggcgcctc ggccagccgg
     7201 ggcgcctttt ttccattgcg cacaaaagtg cggtgccggc tgtctgcgcg gcgtcacagg
     7261 cggttaaagt cgcggaaatc agacggggcg acgccgtatt cccgccggaa cgcccggtaa
     7321 aacgtggaat agtctgcgaa gcccacatcc gtgcagatcc ttcccagggc ctctccttcc
     7381 agaatgcgct ccttcgccgc gatgagacgg ctctgcgtga gataccggta aaagctgatg
     7441 cccagctttt tgcggaacaa ttggtcgatg gtgcttttgc tgacgaaaaa ttcccgcgcc
     7501 gctctctcca atgtaacgtc ctgcgccaaa tgctcctcga tgaaaagatt cagacggtcg
     7561 aacaggtccg gcacctccgc cggaggcgtg ggacggtcga cgtcttccag cgcccgggcg
     7621 atgttcacca gcaactccag cgttgtggca catacgacag cctgccagcc cggtgcgccg
     7681 cgggccgcct cgccgcagcc agcctcaaac agctttgcca ttccctccca acgggtgttg
     7741 gccgtgcgca gcaccccgaa ccgctggttt ttcagcagct ccggccacat cgggcggacg
     7801 ctttccacat attcctggct gaaccataca acgcagcgcc tgtatggatc ggccattttt
     7861 tcggccagca aggggcggtg gctcacaccg ggcggaatga gcacgatgtc accgtggcgc
     7921 aggcggtacc gctgcgttcc cagcagatac tgcgtactgc cgccggtaca gtatatcatc
     7981 tcgtaaaatg tatggctgtg gagctgcacg ccgtcgccgc cctggcttac gtcccggtgc
     8041 gtgtctacaa aacgggagct catttccagc tcctgataga tgttcacgaa ccggaaccgg
     8101 tatttttcca gctgtgcctg ccgctctccg ggggaaagct cccgtccctc tacgcccgcg
     8161 gctttctcca gcattctccc cagctcagac aacttcacaa atcccacctc gttttattgt
     8221 tgattttgtg tatattttaa cttttcttta agataaatgc aataatctat gtgtcatttg
     8281 caattttaat tgcaacaaaa ttgttgtatg ctgtatccat gatacagcgg tgcgccttgc
     8341 cggccgggag gaatgccgca acatgacagg aggaatcaca aaatggctac caccactcaa
     8401 aaaagcggcc tgacgttcaa gcacaaagtc ggctacgcca tgggcgacct tggcggctgc
     8461 atgacctttg cgctgatggg cgccatggtc acccgctatt acaccaacgt gctgcaggtc
     8521 aataccgccg tcctggcgac gctgcttctt gtctggaaca tctgggacgc cgtgaacgac
     8581 ccgatgatgg gcgcgcttat ggacaaaatg tacgcaaaac atcacaataa gaacggcaag
     8641 ttccgcccct ggctgctgcg tgccgctccg ctgctggcca tcacctctat cgtgttctgg
     8701 acagtcccca cgctgttcga cggctacacg atgcttgtgg tgctgttctt ctgcaagatc
     8761 ctttacgagg gctgctacac catgttcaac atccccatgg gttcgctgct ttccgccatg
     8821 gccaacaccg acggcgagcg tgccgccctg tcctctgccc gcggttttgg ttccatgatc
     8881 ggcaacgtga tcccaatgat gatcctgccg cagctgctgg ccaaattcgg cgacacttcc
     8941 aaggctttcg gcatcggcgc cgccatctgc gcgtccatcg gcttcgtgtt ctgcttcctg
     9001 cactattact ggacagagga gcgcaatgtc gcggatacgc ccaaagaaga agccgccaat
     9061 gtcaaattta ccgatatcct ttccgttttc aaaaagaacc gcgcgtttct tgcgctttgc
     9121 atccacggcg tatgtatctg ctgcatgcag tatgtaggtt ccacactggg cacctatatg
     9181 tactccgatg tattgggcaa catcggcatt atgagccttg ccagcgttgt ctccatgccg
     9241 ctgagcatcg tcgccctgct catcatgccc aagctcgctg aaaaattcgg cctggaaaag
     9301 gtcatccgca cctgtctgct catcggctgc ggcctgtata tgggcctgtt cgccgtcatg
     9361 atgctgttct ctgttcctgc catcgtctac ctcgtctgga gcacgatggc cagcggtatt
     9421 gcaagcgtgt ccatcctgat gcagtggggc ctcgtgggcg aagccatcga ctacaatgag
     9481 cttctgacgg gcaagcgcac cgaaggctcc atctacggca ccttcaacct gtcccgccgc
     9541 atcggccaaa ccgtcggcaa ctctgccgcc gtgctggcac tgggctggat cggctacgac
     9601 gccaacctgg ccgcccagtc cgcaggcacg cttaccggca tcaaggcgct gtgcgtgctt
     9661 gtccccggta ttttccttgt cggctcctgg atcgcattcc gctttgtgtg gaacatcacg
     9721 cccgagatcc gcgccaaaat ggccgccagc aaagccgcaa aatctgcaca gtaatgccgg
     9781 ccgccgcgct ttatcaacgc aaaaggagtg taaccttgcc atggaacttc gcacccgttt
     9841 tctgcccaag ctgctcaaca gcgaggtaga tgaatactta aaaagcaacg atatcatcat
     9901 cgtgccggtg ggcacggtgg agatgcacgg cggattgccg ctggacagcg agaccgtcat
     9961 cagcgaggct ttcgccctga aaatggccga ggcctgcgac ggcctggtgc tgacgggcct
    10021 gccttatttc tacgccggtg ccacagcctc cggccgcggt accgttcagg tgagcgttcg
    10081 ccagggcatc gactacctga gcgccattgc ccacagcctt cttcgccagg gcttcaagcg
    10141 gcaggtctac atcagctttc acgggcccgc acacatgacg tgcagcccca tggtgcgcga
    10201 tttcttcgat gagacgggcg tacccatcct gtacatcgac ctgagcatgc agatgttcaa
    10261 gtccgccaaa gacctgttca agtccatgga cgctttccac gccattactg tgggtgccta
    10321 tcagcttctg gggcgtctgg aggacgtgcc cctcaccact gaatacagcg cgccgcatac
    10381 acagacttgc gcgcctttca acgatctgtt cgcactggcc tatcagagcg gctccgcggg
    10441 gtactgcttc gcagacccca tggaccacat gcccaccacc gccattcccg acgaggccac
    10501 gcgcaaagct ctggccggcg aaggagcgga gctgatcggc gagatcgtga agcgcatgga
    10561 tatgccgcac gtcgccgcgc agatgcggga cctgaaagca tatacggacc gcgccgggga
    10621 actgtacccc tggatgccca gcgcatacaa cagcgccaaa tgacacaccg ttcccccgcc
    10681 ccttgatttt cgcagccgcc cgcagcccat gcgggcggca tttttggcct catccgccgg
    10741 taaaatttcc ggattttatg caaaccgctt gcaattcata ctatatcggt atataataaa
    10801 gttagacaaa gctaacacat tgttctgcgg caaacagagc aaatacaaat aattttacaa
    10861 aagaggccat tgaaatgaag atcatcgaat ctctggaaaa acgcaggagc tactacagca
    10921 ttgggaaaga gctgcccgtg agcgaggccc aggtgaccgc gctggtggaa aagcttgcag
    10981 agcttgttcc cgacgcattc aacatgaaga gctcgcgggt cgtcgtggcg ttgggtgaaa
    11041 agcaggacat gctgtggaat acagtttatg acgccttcgg cggcaaggtc gcccgtgaaa
    11101 agatcgacag ctttcgggca ggcgcgggca cggtgctcta tttttacgac gaagccgtag
    11161 tgcaggcgct tcaggcacag tttccgctgt atgccgccaa cttccccggc tgggcgctcc
    11221 agtccagcgg catgcttcag ctcagtgttt ggagcggcct tcgggagctg ggcgtggggg
    11281 cgtcgctgca gcattataat cccatcatcg acaaagacgt ccgcgcgctg ttcggcttac
    11341 ctgaaagcta ccgactggtg gcgcagatgc cctttggctc tatcaaagac gagcctgagc
    11401 ccaaagaaaa agaagacatc tccctgcgcg tgacaactgt acgctgaacc gaatcgcccc
    11461 gttccccgcc cggggacggg gcttttgttt tcgcggcgtc agggtataat aaccccaaag
    11521 ccgccgcttg cgcggctgcg cgtgtgcacg gcacacgccc ctgtgc
//
LOCUS       DAWBSB010000053        11527 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1318_length_11527_cov_3.267172, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000053 DAWBSB010000000
VERSION     DAWBSB010000053.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 11527)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11527)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11527
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1318_length_11527_cov_3.267172"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5203..5212
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2254 a         3596 c         3329 g         2338 t
ORIGIN      
        1 aacccgatgt tgattttcaa tgaattaaac aatgtgttga tattggacgg aaatacgatc
       61 ttggtgaata cctggcgctt tgtggcgccg aaggtgcgcg ccattttaat gctttccggg
      121 ttggtggcac ggaagcccgc cagcatctcc agcaccgtga cgatgaggga aatggccagc
      181 gccatgaaaa tgatggcctc ggtgcccgcg cctacgacga tgatgataac aggccccagc
      241 gcgatcttgg gcaggctgtt gagcactacc agataaggct ccgccacgcg ggacacaaag
      301 ccggaccacc acagcacgac ggcgatggcc acgcccagca gcacgcccag cacaaagccc
      361 gtcagcgttt catacagcgt cacgcccaca tgcatcatca aatggttctg ggacatgttg
      421 cgcagcgttt ccaggatgcg cgatggctgg ctgaggataa agctgtcgat ccaccccagc
      481 cgggcggata tctcccattg ggcaaaaaag aacacaagaa agccgatctg gcacgcgtgc
      541 accagatggg tgcgccgcct tttgcggcgc agatacagcg tgcgctcgcg cgagacggcg
      601 ggcgggttcg tcggtctagc ttttttcatc ggtactcagc tctttccata tatggtcgaa
      661 gtaagggccg aactgccggt tggcccggca cgagagcggc gagcgccccg gcccgaagtc
      721 cacggacagg atctccttta cctgcgcggg ccgcgcggaa aggacgagta tcttatcccc
      781 catgctgatg ctctccggga tatcatgcgt gaccatcagc gtggtaacct gctcccgctt
      841 caaaatgcgg tatacatcgt cggtgacgtt caggcgcgtc tgataatcca gcgccgaaaa
      901 cgcctcgtcc agcagcagga tatccggccc tgtggccagt gtgcggatga gcgccacgcg
      961 ctggcgcatg ccgccggaaa gctggcgcgg gtatttgtgg cggaattcat acaggccgta
     1021 ggttttgagc agctcccggg cgcgggccac gttctgcggc gttttggcgc cgcggatctc
     1081 cagcccgaac agcacattgc ttaaaatggt gcgccactcc agcaaattgt cgcgctgcag
     1141 catatagccg atgtgccttg aaatgcctgt gactacctct ccgtccacat agacccgccc
     1201 ggaagtgggc ggcagcagcc cggcaatgat ggaaagcagc gtgctttttc cgcagccgga
     1261 cggccccacg atgctcataa actccccctt ctgtacagtg aagctgatgt gatcgaaggc
     1321 tgtgacttcc ccgttttcgg cctggtattt gtggccgacg tcctctacgc gcaaaatctc
     1381 catggcgcac tccccctctc ccgccgcgtc ggcgcgcccc gccgcggcat gccggctgta
     1441 acggcgcgcc ggacacccgg cgccgcatac cgtctgttca gccgtgatag tacagtacat
     1501 gcagaacagc cgcgttttga tactcagcgc agcgcacggt tctttctttg cgcgccctga
     1561 aacgggtccg ggctgttttt ttgccgggaa gaggccttgg aaataaaatt tcgtacaaca
     1621 aaaacagccg cggcgagtct ccccgccgcg gctgcttttg tccgcattga ttttattgta
     1681 acggcagcgc gccttcccgg ccgcgttacg cccagccccg ggcgcgctcc acggccttgt
     1741 gccagccgtc caccagctcc cgcgcacggg ccgcttccat ctgcggctcg tacagcttgt
     1801 ccagcgtcca ctggcccttg atgtcgttca ggtcgcgcca gacgcctgtg gccaggcccg
     1861 ccagatatgc cgcgcccaga gccgtggtct cgcggatcat cggacggcgt accgtcacgc
     1921 ccgcaatgtc ggcctggaac tgcatcagca ggttatttgc cgaagcgccg ccgtccacct
     1981 tcagttcttt gaactgcacg ccgctgtccg cctgcatggc tttcagcaca tccagcgtct
     2041 ggtaggcgat gccctccagc gccgcgcgga tgatatggtt gcgcccggcg ccccgcgtca
     2101 gccccaggat ggcgccccgg gcgtacatat cccagtacgg cgcgcccagc ccggtgaacg
     2161 cgggcaccac atacacgccc gcgctgtcct tcactttgtt tgcgaaatac tcgctgtcgg
     2221 cgctctcgtt gatgagcttc agctcgtccc gcagccattg gatgaccgcc ccgcccacaa
     2281 acacactgcc ctccagcgca taggtcgcct tcccgttcag gccgacggcc acggtggtga
     2341 gcaggccgtt tttgctctcc acaggtgtgt cccctgtgtt catcagcata aagcagcccg
     2401 taccatatgt atttttcaca tcgccccgcg caaagcatgt ctggccgaac agggcggcct
     2461 gctggtcgcc cgccgcgccc gcgatgggta tttcggcccc gccgatgcgc gccgcaccgt
     2521 acaccatgct ggaatcgcaa actttgggca gcatgcacat gggaatgtcg agcagcgcac
     2581 agatgtcctt gtcccattcc agcgtatgga tgttgaacag catcgtgcgc gaggcgttgg
     2641 tgacgtccgt cacatgggcc gcgccgcccg tcagcttcca gatgagccag gtgtccacgg
     2701 tgccgaataa aagttcgccc cgctcggccc gctcacgcgc gccctccaca ttgtccagga
     2761 tccacttgat ttttgtcgcg ctgaaatatg cgtcgatgag caggcctgtt ttggccctga
     2821 tctcgtccgc atagccccgc tccacaagat tcgtgcagaa atcggacgtg cggcggcact
     2881 gccacacgat ggcattgtac accggacggc cggtgttttt gtcccagacg atggtcgtct
     2941 cgcgctggtt ggtgatgccg atggccgaga tctcgcccgc ctccatgccg ctgcgggcca
     3001 gcacctccac cagcacgccg tactgggagg catagatctc cagcgggtcg tgctccaccc
     3061 agcccgcttt gggataatgc tgtgtaaatt ccttttgtgc catgttgacg atgttctggt
     3121 gctcgtcaaa aataatggcg cggctggacg tcgtgccctg gtcgagggcc atgacgtatt
     3181 tgctcatgcg gtacgccccc ttccggcggg ctcaaagcgc cgccgtcacc gaaattttaa
     3241 ttttgtattc cggcacggcc agctggccgc ccgtgacgct gcgcgccggt tcatagccgt
     3301 ccacgaccca ggcgtcccac acggcgttga acgcgccgta gtcggccatg tccgccagcg
     3361 tgatggcagc tgtgagcacc cgcgttttgt caatgccgtt gcgcgccaga atgttgtcga
     3421 tgatgtggat gacgtcctgc gtctggcccg tgatatcggc gtccaggtct gtggtggtga
     3481 tgccgctggt gtacaggatg ccgttatgcg acacgctctg cgcgcggcgg ccgtttgccc
     3541 cgttgcgttt gatctccata gttttaccct cccgtttgta tttcgcgccg cgcgcgcaga
     3601 acgcgcgcgg cggcaaaaat ccttttcatt ttcgtgccaa tctgctataa tctaaagtat
     3661 gaacagcctt ttttcacgcc tgccgggtca tactatgcag gtgtttcttt tatcttaaca
     3721 ttttctgcga ggaatttcca tatgaaaaaa tgcgctgccg ggcttgcagc cctgtttctt
     3781 atcgttattt ttctttcgcc gcgcgccttt gccgccgggt acgagctccc cgaaggtacg
     3841 tccatcacgg cgcagtcggc ttattttgtc aacctggaca cgggcatcgt ggtatttgaa
     3901 caaaacgcgg acgagcagcg aagcgtggcc agcctgacca aattgaagac cgcgctgctg
     3961 ctgatggaaa atgtgagcga cctggaaaac accaaaatca ccgccgaacg aagcctgtat
     4021 gtccctccca tcaccaaccc ggattcatcc acggccgaca tttggccggg tgagaccgtg
     4081 agcgcgctga acatgctgta cgcgatgatg ctgccaagcg ccaacgaggc cgccgaggcc
     4141 gtggggtatt acctgggcaa cggcaacctg aacaactttt acgcgctgat gaacgcccgc
     4201 gcggccgcgc tgggctgcac caacaccaat ttcaccagca ccaacgggct tgcagagatg
     4261 gaggcgggca actactcctc cgcccatgat cttgcgctta tcgcccagga atgctggaag
     4321 cacgagattt tccgcaccgt cgtgggtacg gcgacctacc agatgcccgc tgcggaaagc
     4381 cacagcgagc cgtacaccat tctcagttcg gacgctatga tcaagccctc ctctgctgta
     4441 taccgttcct acatcaaggg catcaaaacg gggtccacgc aggccgcagg ccgcaacttt
     4501 gtctccgccg ccgtcaacga caaaggcgag acatacatcg gtgtggtgct gggcgcgccg
     4561 tgggacgcgg ccgaggacgg ctatgcttat tcgtttcatg acaccgccac catctacgac
     4621 tggctgttcg ccaattactc catcaagcct tccatcgaca ccggttcgcc cgtgacggag
     4681 gtcaaggtga attattcctc tgaagcggac acgctgatgc tctaccccgc cggcgacctt
     4741 aagaccgtgc ttcccaacga cagcgacgag ctgctggaaa aaaccttcga tgtgcccgaa
     4801 agcgtggacg ctcccattgc catgggagac aaggtcggca ccgtgacgct gacgctgaac
     4861 ggcgcgctgg tcggcaccgt ggatctcatt gcagggcagg acgtggcccg gaacgttttt
     4921 ttgtatgcgg tgtcacgcgt gcaggcattc ttcggcgggc tttatttccg tgtggtatgt
     4981 atcctgacgg ccatctttct tgtgggatac ggcctgctga cgttctggat gtacaaccgg
     5041 cagaagcggc agcgcgtccg ccgcgcgggc ggcccgggca atgcgccccg cgctccgcgg
     5101 cccggccagc ctccctcagc cgggcgcggc gttccgcctc ctgcgggaaa gccctccgcc
     5161 tcccgtcccc ccagctccgg gccggacgag cttgacctcg gcnnnnnnnn nngcgaggac
     5221 tgacgcccct ttttgttttt gaaataaatg cagcaaatac aataaagaaa gccggaatct
     5281 gtgatgacaa atatcaacga cgcacgcaac ctgacgcttc tgatggattt ctacgagctg
     5341 accatggcgg ccggctattt cgaagaaggc tacaccgaca aggtgggcgt gttcgacatg
     5401 ttcttccgca agatacccga cggcggcggc tttgccattg ccgcgggcct ggagcagttc
     5461 tgtgaggtag tggataacct tcacttcagc gaagaggaca tcgaatacct gcggggcaag
     5521 ggctgcttct ccgagcagtt cctcgactat ctgcgccgct ttgaattcca ctgcaatatc
     5581 tgggctgtgg ccgagggcac gcccattttc ccccacgagc ccatcgtcat cgtggagggc
     5641 ccggccatcg aggcccaact catcgagacg ctgcttctgg tcaccttcaa ccatcaaagc
     5701 ctgatcgcca ccaagacgaa ccgcattgtc cgtgcggcgc agggccgccc ggtgatggag
     5761 ttcggctccc ggcgggcaca ggggtacgac gccgccgtgc tgggcgcgcg cgcagcgtac
     5821 atcggcggcg tggtaggcac cgcctgcgtc atggcggaca ggcagttcgg cattcccgcg
     5881 ctgggtacca tggcccatag ctgggtgcag atgttcccca gcgaatacga ggccttcaag
     5941 cggtacgccg agctttaccc cgacaactgc acgctgctgg tggatactta cagcgtggtg
     6001 aagagcggtg tgcccaatgc catccgcgtg ttcgacgaag tactcaagcc tctgggcatc
     6061 cgtcccaagg gcgtacgcat cgattccggc gacattgcgt acctttccaa aaaggcgcgc
     6121 aaaatgctgg atgcggcggg ctatccggac tgcggcgtgt gcgcgtccaa ctctctggac
     6181 gaatatctgg tacgggacct gatcctgcag ggcgcgcagg tgacgaactt cggcatcggt
     6241 gaaaatatga tcaccagcaa gagcgaccct gtgttcggcg gcgtgtacaa gctggccgct
     6301 gtgaaggatg gaggccggta tcttccgaag atcaaaatca gcgaaacagc ggaaaagact
     6361 accatcccgc acctgaaaaa cctgtaccgc atctacgaca acgacaccgg aaaggccatg
     6421 gccgattaca tcaccatggc ggacgagacc gtggatgtga cggacggcat caccttgttc
     6481 gacccggtgg agacctggaa aaagcgtacc tttaccaacg tgcgcgccga acggctgaac
     6541 cgtcccattt atgtgaacgg aaagcgcgtg tacgaaaacc cgccgctgcg ggatatccgg
     6601 gatttctgcg ccgctcaggt ggcgactctg tgggatgagg tgacccgctt tgaaaatccc
     6661 caccgctatt atgtggacct ttcccaaaag ctctgggacg aacgccagcg tctgctgacg
     6721 gaattcggcc gctgagcgcg gcattcaaag gagaaaacac aaggagaatc atggaaaaac
     6781 agcaaaattc tatggccacg ccggcgctgc tgcgccgctt ctggccctac atggcccgct
     6841 ataagggcat cctctgcttc gatctgttct gtgcggcgct cacctcggtg tgcgagctgg
     6901 ttctgccgct catcatgcgt tccatcacca atatgggcgt aaacgacctt tccagcctga
     6961 cggtgagcta tatcctccgc gttggcgcac tttatctggt gcttcggctc attgacggcg
     7021 cagccaacta ctttatggcc aacacgggcc atgtgatggg cgcgcgcatt gaaacggata
     7081 tgcgccgaga cgcctttgac cacctgcaaa agctgtccga cagctacttt gccaatacca
     7141 aggtgggcca gatcatgggg cgcatcacca acgacctgtt tgatgtaacg gaattcgccc
     7201 accactgtcc ggaagaattt ttcatcgccg ggctgaaggg cgttatctca ttcatcatcc
     7261 tcgcgggcat gaacgtatgg ctcacgctca ttgtgtttgc ggtggtgccc gttatggtcg
     7321 tctgctgcac gcgctttaac catatggtgc gcgccacatt cctcaagcag cgccgccaca
     7381 tcggcgaact gaacgcccgc atcgaggaca gcctgctggg tgagcgcgtg gtgaaggcct
     7441 tcggcggcga ggaccttgag cgcgaaaaat tcgagcagga caacggacgc ttcctggaca
     7501 tcaaaaagga gacctaccgc tggatggccg ccttcaacac caccacacgc ctgttcgacg
     7561 ggctgatgta ccttgttgtg atcgtggcgg gcggcctgtt tatgctctac ggcaagatcg
     7621 agccgggcga ccttgtggcc tacatgctgt acgtcaccac gctgctgtcc accatccggc
     7681 gcatcatcga attcgccgag cagttccagc gcggcatgac aggcatcgag cgcttctgcg
     7741 agatcatgga cgcggatgta gacatcttcg acgacgagaa cgcaaagccc ctggaaaatg
     7801 tgcagggacg catcaccttc gagcatgtga gctttgaata tccggacgac cacaataaag
     7861 tactgcacga tttaagcctg gatgtgcgcc ccggtgaaaa gctggctctc gtagggccgt
     7921 ccggcggcgg caagacgacg ctgtgcaacc tcattccccg tttttacgac gcaacgtccg
     7981 gccatgtgtg catcgacggc cgcgatgtga aaaccgtaac gctggaatcc ctgcgcaggg
     8041 ccatcggcat cgtgcagcag gacgtttatc tgttcagcgg aaccgtgtac gaaaacatcc
     8101 tctacgggcg tccggacgcc acccgcgccg aggtggagca ggccgcaaag ctggccggcg
     8161 cgcacgagtt catctcggcg ctgcacagcg gctacgacac ctatgtgggc gagcgcggcg
     8221 tcaagctttc cggcgggcag aagcagcgca tctccattgc ccgcgtattt ttgaaaaacc
     8281 cgcccatcct cattctggac gaggccacca gcgcgctgga caatgaaagc gaacacctgg
     8341 tgggccagag cctggcgcgg ctggcccagg ggcgcaccac cctcaccatc gcccaccgcc
     8401 tgacgaccat ccagcacgcg gaccgcattc ttgtgctggg cgagaacggc atcgaggaag
     8461 agggcaccca cgaagagctg atggcaaagc acggcgttta ccacaagctg tggacgggcg
     8521 ccgccgcgct ggacaagccc atgatgtaaa taacgttttc ttttccgcac atccacaccg
     8581 aaacagcaaa ggaggcaccc ttggaacaac cgctcgtatc catcgtcatc cccgtgtaca
     8641 acgcggcgcc ggaccttgcg cgctgcatcg agagcgtccg caaacagacc taccgaaaca
     8701 tcgaggtatt cctcgtcaac gacggcagca aggacgcttc cagccccatc tgccacatgt
     8761 acgaacgcat cgacccccgt gtccatgtca tcgacaagga aaacgcgggc gtttccgccg
     8821 cgcgcaacac gggcatcgcg gccgcacagg gcaaatacat ccagtttgtg gacagcgacg
     8881 attaccttgc tccgggcgcc acggccacgc tcattgagcg ggccgaacac gcgggtgcgg
     8941 acatggtgat cgcgcattac taccgcgtag acggcggcaa aaccacccgc cacggcttct
     9001 taaaacgccg cgacgtgatg gaccagcgcg aatttgcgcg ggagctgatg gacgaaccgg
     9061 ccagcttcta ctacggcgtg ctgtggaata agctgtaccg cacggcgctc atccgggaca
     9121 acggcgtgct gtgccgggag gaactgaact ggagcgagga tttcctgttc aatttggaat
     9181 acatccgcta tgcccaccgc ttctgcgcgg tggatacccc ggtgtattat tacgtcaaaa
     9241 acgaaaaaag catcactcat acacaaatcg accttgtagg cgtggtgaaa accaaggcca
     9301 agctgttcac ctattataaa ggcctgtacg aaagccttgg cctgtatgag aaattcaagc
     9361 cgcagatcta caaatatctg gtggccacgg ccgagcatcc atgacgggag ggcgcgcggc
     9421 accatcggcg ccttaccgcc ccgcctgtac tgtgcgcggg gctttttgca gcctgaaaca
     9481 ggggggattt tgaatggact ataaaaaaat gaccgaaacg cgccagagct gccgcgcgtt
     9541 tgaccctgtg cgcacaccgg accgcgccgt gctgacgcag gtgctggaag ccgcgcgcct
     9601 tgcgcccagc gcctgcaaca gccagccata ccatttttat gtggccgtag acggcacggc
     9661 ccgtgctgtg ggcgccgcca cccaaagtct gggtatgaac aaatttgccg gcgactgtcc
     9721 cgcgttcatc gtcgtggcgg agcagcccta caaccgcagt gccgccgtgg gcgcaaagct
     9781 gaaagaccag gactaccgcg ccgcggacat cggcctggct gtgtcccagc tcgtctaccg
     9841 cgcccacgaa ttggggcttt ccacctgcat cctgggctgg ttcgacgaga aaacgctgca
     9901 ggcgctgctg aacacaaacg cccgtgtgcg tcttatcatc gccatcggct atgcccgcgc
     9961 ggacgatgcg ctgcgcacca aaaaacgcaa atccctggaa gaacttgtca cctggctgtg
    10021 acggctcctt ccgtgaagaa aaggaggata cgcacggtga agctctgcat cgcgtccgct
    10081 ccatgcaagg tctgacagga cgagcctgca tggagcatct tccccgcatt cgtcccggcg
    10141 gtgtgcggtt cgcatagcgg tccacacccg gcagactttg catttttgga ttgaagtatt
    10201 tcaaacaaaa aggagcaaag tcgatatgag atctttcaaa gtactgatca gagagctgcg
    10261 cggctttttg atcttatggc tgacccagtc cttttccgca ctgggcagcg caatgacgaa
    10321 tttcgccctc atcgtctggt cttaccaggc gcagggctct gcactcacca cagcgctttt
    10381 gtccgtatgt tcctatgcgc cctatgttgt gatgagcatt ttcgcaggcg ctctgagcga
    10441 ccgttggaac aaaaaggccg ttatgctggc cagtgacagc tttgctgcac tgtgcacggt
    10501 cgctgtgctg gtactgcttc aggccggacg gctggagatc tggcacctgt actgtctcaa
    10561 cgcattgaac ggactgatga acaccgtgca gcagcccgcc gcggatgtag ccatcagtct
    10621 gctgacgccc gaaagacatt accagaaagc cagcggcctt cgttcgctcg ccaactctct
    10681 gatcaacatg ctgacgccga tgtttgccac ggcactgttg gcgctggccg gcatccatgc
    10741 cgtcattttg ttcgatctgt tcaccttttt tgccgcgttt ctgtcgctgc tgtttctggt
    10801 gaagctgccc gcagccccct ccggcgctgc ccgtgcagag agcgtgcttc gttgtgcccg
    10861 gcaggggctg cgcttcttaa agggacagcg gggcatcctt cacctgattt tgttcctcgc
    10921 cgccatcaat ttcaccgcgt cggtctacaa cgcggcattc cccgccatgc tgctttcccg
    10981 ccaaggcggc ggcgaggctg ctctgggtgc cgtcaacacc gctacgggag ccgcgctgct
    11041 tttgggcagt gccgccgcct cggcgctgcc gccgcccaaa agccgtgtgc gggcggtttg
    11101 caataccctg ctgctggcca tgagtacgga aaactttttt cttgccttcg gccgcagcgt
    11161 ccccgtctgg tgtctgggcg cggtattggg ctgggccgcc atcccgttga tgaatgccaa
    11221 cctggacgtg ctgatgcgca cccacatccc cattgccatg caggggcgtg tgtttgctgc
    11281 gcgcaatacg ctgcagttct tcaccattcc gctggggtac tttgccggcg gcctgctggt
    11341 ggacaaggta ttcgagccgc tcatggcccg ccgcccggcc ggaagcctgc tgtgcgtttt
    11401 gttcggtgcc ggaaagggct cgggcgccgc acttctgttc ttcttattgg gactgctggg
    11461 cgctgccacc tgccttgtgt tccgtcggga caggcagatc tgggcgctgg aatgtccgga
    11521 cggggcg
//
LOCUS       DAWBSB010000054        11522 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1319_length_11522_cov_3.436906, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000054 DAWBSB010000000
VERSION     DAWBSB010000054.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 11522)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11522)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11522
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1319_length_11522_cov_3.436906"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    573..582
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2226 a         3716 c         3308 g         2262 t
ORIGIN      
        1 ttttgactcc atgatggcgc attacggcaa attttccggg gtaaagaact atatcgctct
       61 cgtgggtaaa aaatccccaa agcttgagga agcattggga tattatggag aaaaaattgt
      121 tttgaacgta caggaactag ggctcaacag ctgctgggtg gcaatgactc atggcaaaag
      181 ccaagcggat gttcgcaagg gagaaaagca aatatgcctg atcgcgtttg gtcatggtga
      241 aacacaaggc gcgccacata aaaacaaacc attgcaggca gtctgtaatc gtacggaaaa
      301 tatgcctgaa tggtttttga atggtatgga agccgcactt cttgcgccga ccgcaatgaa
      361 ccaacagaaa tttttctttg aattgttttc tgacagtaaa gtcaatgcgg tttgcggtaa
      421 gggcttttac acaaaactgg atttgggaat tgtaaaatat cattttgaga tagcttctgg
      481 cagaaaactg caatcatgag tcaacagaac tgctgcccaa cgggaaataa aaacgtttgt
      541 ttttccggct gtatgctcat aagtcaaata aannnnnnnn nnaaatattg aatttcaaac
      601 cgccgtttct gttttccaaa aaagaatatg caaggtatgc taaagttgct gcctgagagc
      661 cgaaagcggc agaaacggta ttgcagtatt attccggata tattaagcag cggtccgctt
      721 gtcaggagca tgtcaacgac ggcattcaag gccgtccagg ggttgtttga aaatcaaaaa
      781 agctgtattt cctccatttt caaacaaccc taatgggacg accctttttg caacaccctc
      841 aatccgctgc gcttgccgcg gaaacagagc cgctctctga ggcaggcgca ctttccggag
      901 cgctctcctc ctctgcgggc agcatgccgt ccagcgtcat gcccagcacg cctgaaagcg
      961 ccgtcgcgtt cagcgtgctc acggcgttgg aatccgccag ccgggcgtaa taatctccgt
     1021 tcgcatcgac ggcgcccagg tacagcaccg aggtgacgct ctcctgtgcg ccggaggcgt
     1081 cgtccgtgcg cgtccactcc acggtgagcg tggcggaggg ctccgccaga ccatatgccg
     1141 ccagcgcctc gtccgttggc ttaaaatccg cgctgctgct gaaatacagt gtggccgcgc
     1201 tgctgatgaa tgcctgcgcg gcggccgtat ccggcgtttc agtacgtgct gtgtcggatg
     1261 ccgtatatgt tttgttcccg tccgcgtcct ccgatacgtc caccgtcagc gtattgccgc
     1321 cttgctccag tgtggcgcgc gttacgctgt cggtgctctc tacgggccag ttctctgtct
     1381 gctgcaggcc gcgctccgta cgcgtaaagg ctgcggcaaa gctctcggat accgtatata
     1441 cgggttcttc gccctccagc ttgatatagt attctccggt gtgggtattc tgcgccccca
     1501 ccagcaccgt acaggtctcg cccgcatcgg tcgccacttc aatgcggttg gacggctcgt
     1561 ccagcccgta agccaccagg tcggccccgc tctgtgttac cagggcggtg gcattcagcg
     1621 gggcgaggta attcgccatg ctctcggcgc ttgtctggtt cacagggaaa tccgcgtccc
     1681 gccggagcgt ccaggtgtca tccaggtatt ccagcgcgat ctcgccgccc tcattttcgt
     1741 aacaaatcac gctgacggcg cccgtgggcg aaaacgccgc tacaccggcg gcggaggaac
     1801 tctcctccgc ctgctccgcc cccgtcaaca gcccatagcc cagcccaagc gccgcggtga
     1861 gcgccgcagc gcccagcagt acggctgttt ttctgttttt catcagcgtt tcctcctcat
     1921 ggcccagatc accacgccga ggacgagcat cgcgcccgga attgcgaaca tcaacagccc
     1981 gctccacagg ctgcccgcgc cggagggtac cgtcagcgtt tctgtcgtaa gggattttgc
     2041 gtggattgtg atcccgctct cgctgcccgt cagccaactg aaagcattca gcaccaggtc
     2101 gctgttggag ccggagacca tctggtccat ggtctcgtcc agaagaccgc tggtggagag
     2161 ccacaccacc ttgccgcccg tctcactgtt ctgcgccgca gccgccacgt tgaacgttcc
     2221 cgtcgggtcc ccgtccgcct gagcaatgga ttcggcgttg taggcatctg ttttggaata
     2281 ggcgctggcg gaagtggcca gcagcgcgtc caccgagaca ttctccggca tttcgtccag
     2341 caccgtgatg gcgtgggcca gcggtgcgag cacatagctg ccgctgtcca ccagcggctg
     2401 agtgacgtca tggctcttga tattgggcag cagataatac ggatagccgc tcaaaaagcg
     2461 gttctggtca ttttcaatga cgatgccgtc ccgcgcgccc atgccgtatt ccgccagcag
     2521 cgccgtcagg ttgggcatgg cttccgtgct gtaatcaccg tagttcgtca ccaggaacag
     2581 gctgccgccg cccgccagat aggtgcgcag gcgctcggcc tcctcggcgg aaatatcctt
     2641 tgtgggcgca ttcagcacca cagccgccgc atcctcaggc actgcgtccg ccgtcagcag
     2701 cgcaagcgtt tcaaagctca tgttctggcg ctcagcgccg tccgtgagcg tggcggaaag
     2761 cccggcttcg ccgtgaccgg acaaggtgta cagcagcggc gcttcctcgc tggccacata
     2821 ggaaagcgcg ctcgtcaagg ctccctcccc gtcaaattcc gtatcatagg tataagtctg
     2881 gtagttgggc gtataggtgt acaggtctcc tgcgtccacc acacggtagc gttcaccgca
     2941 caccgcgatg atactgccca ccgcggcgtc cgccgcatca tacgccgcgc cgaaggtggg
     3001 ataaagcacg gggtctttct gcaccacctg gatgtgggat gaagcgtctt tgtacttgtc
     3061 cagcagctcg gtgacggccg catcctcctg gccggtacgg cccaggtagt agaacgttac
     3121 gtcctgcgtt aaggccgcgc acaggtcctc tgtctcctgt gaaaggctgt acagcttatt
     3181 ctccgtcata tcgtaccgca gatacttcgc aggcagcgcg cccacaaaaa ggttcagcgc
     3241 aaccaccagc gccagcatca cggcgcacag cacgcttgca taagcgccgt ttttaaatgc
     3301 gcgggagtgc agcgcgtccc gcaccgtgcg ttcattttcc tggggtcgtt tgttgtcctg
     3361 catcgttcgc tcctcctttc tcacgaccag cggcgttttt caaacccctg gcaggtgaag
     3421 aacacaaaca gcagcgccac ggaaacaaaa tacaccaggg aagaaatatc gaacaggccg
     3481 ttgacaaaat tgtcgaaccg gccgaacagc gccaggctgt tgagaagctt cgggaacaga
     3541 ccctccagcc acgcgggctt gaggaaataa acggcggcca ggccgccctc cagcacacaa
     3601 aacacggcgc tgggcagcac aaggcttttc gtcatccagt gcaggaacag cgccagcaga
     3661 gcggccagca ccgtgaatgc agccagagaa gccgcggcgc tgccggaaat cagccccgcg
     3721 atgctgggca tcagatacaa aaacaaaagc gagccgaacg tgcatacagc ggcgatgatc
     3781 tggttttccg tcaggctgct catgaacagg ccgatggcaa tggccgccgc gcccatcaga
     3841 aaaaacgcca gcagcgccgc atagctgcgc gccagcgaca ccgagccgta ggcgcgcatt
     3901 accagcgggc atatccccac aatggccagc gggatggcgt acaccgtcac cagtgccaga
     3961 tattttcccc acacgatccc cgcgatggag gcgcgggagg taagcagaag ctggtccgtt
     4021 ttctggcggc gttcctcaac gatgctccgc atggtaagca gcggcaccac cacgagaaag
     4081 atgaatgtgg tgttgtaaag caccaggctg aaatcggcgt agccggactg caggcagatg
     4141 gccgtaaagt acaggcctac cactacaacg acaaacgcgc acagtaccca gccgatcatc
     4201 ccggtgaagg cgctcttcag gtcttttttg tacacggctt tcatttgcgc tcctcctccc
     4261 cattttccgg ctgatgtgcg tgcgctgtgc tttcctcctc ggacgcaatc tcttccggtg
     4321 cgtcgcccgc catttccagc gctgcctgtg cggcggtccc gtcttcatcc tcgtccgcgc
     4381 gtcccgacgt ctcttcttca ccggcctccg gctcgtcccc ggtcagctcc aggaacacat
     4441 cctccaggct tgttgtttcg gcgtccatgc gcagcacggc caggcccgcg cccgccagcg
     4501 caatgcccgc cggtgcgcgc agatccgcgc cgccagagca gacgatgcgg gccgtgacgg
     4561 cgttttcttc ttcgcaggga acaaattcga cagcctgcgc tccctgctcc agcagcgggg
     4621 acagcgcggc ctgcaccgcc tcctgcgttc ccagcaccgt gatgcgcagt gcggcgtgcc
     4681 cggccgcgtg agctgtcagg ttttcgggcg tgtcgcaggc aaccagcttt ccgtggctca
     4741 tgatgagcac cttgtcgcac acggcgttca cctcggaaag gatgtggctg gacagcagca
     4801 cggtatgttc ctgccccagg gagcggatga gctcgcggat ctcgatgatc tgctttgggt
     4861 ccaagccgac ggtgggctca tccagaatga tgacgggcgg gtcgcccagc agcgcgcagg
     4921 caagccccac gcgctggcgg tagcccttgg aaaggttgcg gatgagcctg tcggccacgt
     4981 ctgcaatatt ggtgcgctcc atgcactgct ccaccgccgc cttgcgctgc tcccgcggca
     5041 ggcccttcag ctcggccgca aaggccaggt attcccgcgg cgtcatatcc gtatacagcg
     5101 gcggctgctc gggcagataa ccgatcttgg cgcgggcctc tgcagggctg tcgaaaatat
     5161 cgcagccgtc gatctcggca gtgccgtccg tggcggaaat atagcctgtg atgatgttca
     5221 tcgtcgtgga tttgcctgcg ccgttcggcc caagcaggcc gtagatctgg cccttgtcca
     5281 cggtgaagct cagcgcgtct accgccgtat gggcgccgta gcgctttgtc agttccttca
     5341 ctgtgatcaa acgtcccgtc tcctttactc acaagattaa aaataatttt accgccggaa
     5401 tatgaaaaac cggcgcaatt tttttgatag atttgtgaaa acggagcgga cagggccggt
     5461 tttgttgtca gtccggcgaa aaagtggtaa actggagccg tccagagctg tttttttggg
     5521 ggtacgccta tggaacctgt tttgtttgcc gctggctgtt ttaccgccgc agtcgccctc
     5581 gccgcgggta tttatgcttc ctttaccgcg cgcgggaagg ggcctgtcct ttccaattcc
     5641 tattggttca gcgttttaaa gcgggaaaac gccaataaaa aggccgaata ccgtttagtg
     5701 accgtcgtgt tttcctgtct aagcgcaatt tttctgctgc tcacagtgga aattttcacc
     5761 caatggccct ggacagccgc attgcgctgg gccctgatcg cgtttgttct tatttacgcg
     5821 cttgccgacg cgttccgcac cgttctgaaa ggttcaaaaa cctagttgtc catacgttaa
     5881 aagggccgaa gccgtttatc acggcttcgg cccttttctt atcgtccttc ctgctgttcc
     5941 gccgcctccg gcaggcggaa cacccgcgcg cccagcagcg ccgcgtcgcc ctcctcgtgg
     6001 gtgatgcaca gcagcgtgcg cccggcctgc atacgccgca catatgcggc ggccgcctgc
     6061 cgcgcggctt cgtccaggcc cttgaacggt tcgtccagca ccaccacgtc cgccggagcc
     6121 agcaaggccc gcagcagcgc cacacgccgc cgctgcccgc cggaaagctc gccgacaggc
     6181 ttctccacat cctgcggggt caggcccaca gccgcgaaag cctccgccat gcgcggctca
     6241 tccagcgccg caccgcaggc aaaccgcagg ttcccctgcg cggaaaggcg ctccaccagc
     6301 ctgtcctcct gaaaggcggc tgcaaagcgt tttccctttg caccgcgcac cgcgcccgca
     6361 tcgggctttt ccagccccat aacgatgcgc gccaacgttg ttttgccgag gcccgagcgc
     6421 cccatcacac aaacggcttc tccctgtgca aaccgctccg aaaggccgcg cagcacctgc
     6481 gtttcgccaa acgccttgga cacgttcacc agttcgatgc tcatgtgccg ctcctttcca
     6541 gcaaaacgct ttccacccgt gcaaggcccc acaaaaacag cctttcaaac gcaaggctca
     6601 gcagcacgat caccacggtc catgccaaaa gctcggccgt ggaaaacaat atctttgcat
     6661 tgtacagcgc ttccccgatg ctgccgctgg tgatgccgat gacctccgcc gccacgccgc
     6721 ttttccagca aaggccaagg cccaccgtgc aggccgagcg gaaatacggc agcgcggcgg
     6781 gcacgtaaag ataccgcacc cgctttgccg gcggcacgcg gaatacggcc gccatttcca
     6841 gcagccgggc gtccgtttca cggatgcccg ccagcgtgtt cgtatacacc acgggcagca
     6901 ccatcaaaaa gctgatgaac accgccagat tgcccgcgct gcgcagccac atcagcgcaa
     6961 gaatgataaa actggccacg gggatggatt tgatggtgag catcagcggc cgcagcagca
     7021 cctccaccca gcgcagcgcg gcggccagcg ccgccagcgc aaggcccagc gccagcgcca
     7081 gcaggaagcc gccgatgatg cgcccggcgc tgaacgccgc cgtctgccag aaatccgccg
     7141 tgggcagcag cgccagcagc gcaccgcaca cctccagcgg cgaaaccaaa atcagctttt
     7201 gcccgatgca gacgctggcc gcctgccagg cacaaagcca gaagcagaca gccagtgtgt
     7261 ttgcgattcg gtttttccgt ttgttacgcg ccataataga atgcgtcgtc gggcagcgtg
     7321 ccgcccacgg cctcggggtt ctggtcgtac agcaccttca ggtaaccgct caccttcgct
     7381 ttcatatccg cgccgctgat gaaagtgata ttgcactccg gcaacgcctt ttgcgcgatg
     7441 ggctccttgg gcacgatctc atagcccgcc accagcgcgg cagcctcgtc cacattggcg
     7501 ttcacaaact cggtgctggc cttgtaatcc tccaaaaact cgttaaatgc ggcctcgttc
     7561 tgctccacaa actctttgcg tgctaccaca acgcctgtca ccagcgcgga atccggcgag
     7621 accttgtccc attcctcggt catatccagc gccaccttca ggccttccat ctgcatctgg
     7681 gcagtggtca catagggctg aggcagcatg gcaacgggat agccgctgga cgcctgcatc
     7741 gccgccagca cctcggtggc ctcgctcatg tattcgatgg tcacgtcctc gcccggggta
     7801 aggccattct gcgtcagcac atagttgagc acatactccg gcgtggtccc cttgcccgtg
     7861 gtgtaaatgg ttttgcccgc caaatcggcc acgctggtga tgtcgtcgcc tgtggtgacg
     7921 acatacagca cgcccagtgt gttgacggcg gccacttcga cggcgccccc ggtcttgttg
     7981 tacagcacgc cggccaggtt gcacggcacc agcgccacgt cgatatcgcc ctttataagc
     8041 tgcggggcga tctcatccgc cgcgccgtac atggtcactt cataatcgtg gcgggcctcg
     8101 cccttgtcgg cttcgtccat cagctttaca aggcccatgg tcgtgggccc tttcaggctg
     8161 gcgatgcgga aagccgtgcg ctcggccgca ggtgtgcctg tgctctcggg cgcggcgctg
     8221 cctgcggcgg cagaggacga aacagcgggc gcaccggaga cggacgccgc agaagaagcg
     8281 ggcgcaccgc tgcagccggc caaaaggccc agcgcaactg ccagcgccag cgcggcagaa
     8341 agcatttttt tcatgttttt cataactcct tttcccttgc cggatctgcc cggccattcc
     8401 cgcgcgcaca aattcataca gcgcgcttgc ggtctgtgcc ctattctact ataatagagc
     8461 aaggaatact gttgcagcag caacaaaagg tgaaaaagat ggaagaaaat ttaactatct
     8521 tacggcgctg cagtctgttt gccggccttt cggacacgca gacagccgct ttgctgcaaa
     8581 gcctgcatcc ggtgcgccgc agcttttcaa agggcgaggt gctgctgctg gccgggtacg
     8641 agacgcagga gctgggcgtg gtgctctgcg gacagatcga ggcggaaaaa cagacggcgg
     8701 acggcgtgac gcttacgctg acgcgcatgg ggccgggcgg catcttcgcg gacattctgg
     8761 cgggcagccg gggagcaaaa agcccggtga ccgtgacggc cgcaacggac gtgacggtac
     8821 tgctcatccc ctacaaaagc ctgctgcggc cgggcgttcc gctggacgcc gcccacgcgg
     8881 cagtgctgca aaacctcgtg gcggccatgg cggacaaata cttcgcgctg gaccggcggg
     8941 tggagcttct gatgctgcat tcgctgcggg aaaaggtgct gcactatctg cgcaccgagg
     9001 ctctgcccgc gccgggcggc gctgtgcgca cgccgtacac ccgcgccggg ctggcggcat
     9061 atctgggctg cgagcgcagc gctctgtgcc gggagctttc gcgcatgcgg cgggacggaa
     9121 tattacaaat cgacgggcgc gtgtttcggc ttctgccggg cgttccgccc cgtcaaaacg
     9181 gcaggcccgt gcgggcagac agggaggaaa acgatggaat gtaaacattc aagcggtgtg
     9241 acacagcgtc ttgcgcgtat cgcggggcag gtaaacggca tccgcgacat ggtggaggag
     9301 ggccgcccct gcgacgacct gctggtacag ctctccagtg cgtccagcgc gctgacgcag
     9361 gtggcgcggc tcatcatggc ggagcatctg gagcactgtg tgctggcggg ctttgaaacg
     9421 ggcgaccggg aaaccactgt ggaaaatctg aaaaaagcgc tggagcaatt ttcaaaaatg
     9481 aaatagcaaa agggcgggga actccccgcc ctttttgtgt tccgccgtca ggtacgctct
     9541 gcctcctggc gtgcgatggc tttctccagc cgctcgcacc aggcggcatg ctcctcctcg
     9601 cgggccgcat ccggctgcgc gggtaactgc ttttcgcatt cctccagctg caggcgcagg
     9661 ccgtcggcct tatccgtgaa ataccggatg gctgtgatcg tcccggcctc atcccgaaca
     9721 tacatgcgtg gataccagtt ccacgtctcg ccaaagtctt cggaaacaag cgacacattc
     9781 tccagcgtga ttccattcag cggcggaaag cagcgaatct ccgttgtcgc cccctgcatg
     9841 aaaatgcagc agccaagata cccgccatag ggcgcatcgt gcagcgcggc gcgaaagtcc
     9901 agccgctgcc acgtttggcc gccgtccacc gtgcgcagca cgttgggctg cggagtaaac
     9961 cgctcgccca tttcataccc ggcggtgata tatcccacag aagcgttcac aaaacgcgcc
    10021 cgcctgacgt gggtattctc cggcagagga acgggccacc cctgcaccgc cccccgctcc
    10081 agcggcttgt cgggcgcgcc ctcgcagcgc cagagccaga gctttgcggt ttggctttca
    10141 cccgttccgg catatacgcc cgtgatcaga tagaacaggt cgtcccgaac gacctccatg
    10201 ctgtatatgt tttcaatcgg tgtgttctgc ccttcaccca ccaggtccag cggttgagcc
    10261 gtccactgcg cgccgccgtc gcgggaggtg tacagcgtca cgcggccggg gtgctcctca
    10321 tccgcatcac acgctgcggt aaaccatgca tcttcccggg gccagtaact gccgccgtac
    10381 aggatattgc gttttccctc ccaccggggc gcgggcgcgt acacctcgct gccgtcggcg
    10441 ctgttgtaag ccatcacgca ttcgccgtcc cgccccggat aaaccgatgc gccgtaaacc
    10501 agccagtgct ccttcacgtt ttccatggac ccggcctgcg cgggcagcgc cgcggccttg
    10561 ggctcgcttt ccgcggattt aacggaaacc gacggctgtt cggcactgca tgccacaaag
    10621 ctgcctgcca gcgcagccag gcagaggatg caggccagtg ccgcgcggcc cgcgcgcttt
    10681 cccgtcttgc tgaaaatggc cgaaaagcgg cgcagcatca tttttttacc gggcgcaaag
    10741 caggtggtga acggcgcggg cctgccggct cccgccttca aagcggccag cagagcttca
    10801 ccataagccg cccggacagg tgctcccagc tttgttccgt gcagcacggc ttcatcacag
    10861 gcgaattcca gctccttttc gcaccggcgc gccagcagcc ataccgccgg gttataccaa
    10921 tgtacggcgc acgcagacag cagcaccgct ttgcagacga tatgtccgcg ctttatgtgc
    10981 gtcaattcat ggcggagcat ccattccaga agcgggcttt catacagggc ctccgggacc
    11041 agcaccaccg gacgcagcac gcccacggcc atagggcacg gcacggcggc attggcatat
    11101 atccccggtg cgcggcgcat acgcaccgcc cggcaggccg cggcgtaagc cttccgcgcc
    11161 gcagagcttt ccatcggccg gttccaatgg aacgtgcggg cgcgccatac cgcataatgc
    11221 gcaaggtgcc acagcgcaca tacgcccacg cccgccagcc acacaaagcc ggccagccgc
    11281 gccggcgcag gaagaaggat ctctccggag cggaggacgg gagttttgcg tgatgctccg
    11341 gaagcctccg tccgcccttc ctcctgtgca gacggtacgc cggcagccgg ttcccgcggc
    11401 gccgcctgtg ccgttccaaa ggcttccttt atttccggtc ccgtatttct gccggatgcc
    11461 tgcggtacgg aggctacgct ttcccccgtc cccgcagcct ggcccgtggg cgcgccggct
    11521 gc
//
LOCUS       DAWBSB010000055        11426 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1328_length_11426_cov_3.676106, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000055 DAWBSB010000000
VERSION     DAWBSB010000055.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 11426)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11426)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11426
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1328_length_11426_cov_3.676106"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5319..5328
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5958..5967
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2288 a         3719 c         3381 g         2018 t
ORIGIN      
        1 ggcacaccct gtgcgcttac gcggcagcac ggctgtgcgc gcgggtagat tcggcccgtg
       61 ctggggaaag tatgaatggg ttgggggctt tttttgatgc gagttgtcgt aaaggtgggc
      121 acttccacgc ttgcccacaa aaccggatgt ctgaacatcc gcctggtgga aacactgtgc
      181 aaaacattgg cggacctgca gaatgccggg caccagatcg tgctggtttc ctcgggcgcc
      241 atcggcatgg gcgtgggcaa gctgcagctc ggggcgcgcc cctcggacat ggccaccaag
      301 caggccgccg cggccgtggg ccaatgcgag ctgatgtaca cctacgacaa gctgtttacc
      361 gaatacaacc acaccgtggc gcaggtgctg ctcacggcgg cggacatcga ggacaaaatg
      421 cgccgccaca acgtggaaac gaccctccag cgcctgctgc agctgggcgc cattcctgtc
      481 atcaacgaaa acgacacggt ttccacagct gagatcgcag aggtctccac cctgggcgac
      541 aacgatacgc tggcctccat tgtggccgtc agcgttgaag cggacctgtt ggtgctgctc
      601 agcgacatcg agggcctgta caccgccgac ccgcacaaag acccgaacgc ggtgctcatc
      661 cgtgaggtca ccgccatcac gccggacatt gaggcgctgg cggggggcgc gggctccggc
      721 cagggcacgg gcggcatggt gacgaagctg cgcgccgcgc gccgtgtgac acagcagggc
      781 atccccatgg tcattgccaa cggcgcggac ccgctttgcc tgtacgacat cgttgaggga
      841 aagccggtgg gcacacgctt tctggggaat gcccaggcct gacgcgccgc gcccattgaa
      901 cgtcccgttt taaaggagga aaccgacatg accaccgccg atctgctttg cgccgcccgc
      961 gccgtaaaac cggccgcggc cgcactttcc accgctgaga aaaaccgtct gctggaggcc
     1021 atggcccagg ctttgctgga ccacgccgat aatatcctcg cggccaacgc cgcggatgtg
     1081 gaggccgcgc gcggcgtgat cgcccctgtc atgatcgacc gtctctctct ctcgcccgcg
     1141 cgcgtggaaa gcatggccga gggcatgcgc gccgtcgccg ccctgccgga cccggtgggc
     1201 gagctcctca gcgagacagc ccggcccaac gggctcgtca tccgcaaggt tcgcgtgccc
     1261 atgggcgtga ttgccattat ctacgagagc cgccccaatg tgacggcaga cgccgccgcg
     1321 ctctgcctga agagcggcaa cgtatgcgtc ctgcgcggcg gcaaggaggc catccgctcc
     1381 aatacagccg ttgtcgccgc gctgcgcgcg gggctggagc actgcggagc gcccggcacg
     1441 ctggtgaatc tggtggagga tacctcccgc gccagtgccg cccacctgat ggaggcccgc
     1501 ggctttgtgg acctgctgat cccccggggc ggcaagggcc tcatccgcgc ctgcgtggac
     1561 agcgccaagg tgccctgcat cgaaacaggc acgggtatct gccatattta tgtggatgcc
     1621 gcggccgacc tgcaaaaagc cctggacatc gtatacaacg cgaaaacgag ccgtccgtcc
     1681 gtgtgcaacg ccgccgaggt ttgcctggtt caccgggacg ttgcagaagc gttcctgccc
     1741 gcgttggccg cgcgtctggc cggtaaacag gtggagcttc ggctctgccc ccgcgccgcg
     1801 gccgtcattc ccggcacgcc cgccgggccg gaggatttcg acaccgaatt tctggattac
     1861 attctggccg taaaggtcgt ggacagcgtg caggacgcgg cggcgcatat cgcggcccac
     1921 tccacgggcc acagcgaggc catcgtcacc gaagacgccg ccgcggcgga cgcattctgc
     1981 gcagccgtgg attcggccgc cgtatatgtg aacgcctcca cgcgctttac ggacggcggc
     2041 gaatttggac tgggctgtga aatgggcatt tccacgcaga agctgggcgc gcgcgggccg
     2101 atgggcctgg ccgagctgac cagctacaaa tatatcatcc ggggcaacgg acaagtgcgc
     2161 tgaacgcccc gctttcaaca aggagagtac cgatatggca acttcaaacg aagccgaaaa
     2221 ggcgcgccac gcgctgcgcc gccgccgcag gctgcggcag gtgctgggcg cggtggtgtg
     2281 tgtcctggtc gtcattgggc tggcaagcgt catttccggc ggcgtgcgcc tgacagccag
     2341 cctgttcgac gacaccgagg aaaaagagga attcgaactg cggctgcaaa cgcttgtctc
     2401 gctggacccg gagccttttg attcactgga aagcgcaaac cgcgcgcgcc tgctggacgc
     2461 ggccatctgg tccgttatcc agcaaaacag cgacagtctg gacacctacg agcgcgacga
     2521 ggtgggcgcg atgtacctgc ccacactgga tatcgaccgc gccgtggcct cgctgtacgg
     2581 gccggatttt catttcaatt acgagacctt caccgacaac gggctgacgt tccagtatgt
     2641 gccggaaaag aacgcctacc tgctgcccat caccagcgtg aacggcaact attcgccgcg
     2701 cgtgatgcag atccggcgcg aaggcagcac caagcgtgtg accgtcggct atctggacct
     2761 gtacggcagc ggcgagatcg tgttcgaccc caccaagctg gaaccggcga aatatatgga
     2821 ttatatcttt acgagaagcg gcgacgcgta ttacctgaca gccattaccg aaagcgagat
     2881 gaaagcagag agccagacgg gttccgtctc ttccggtgga gcgcaggcgc ctgttgcccc
     2941 gcaggaccag gtgatggata atgtggcctc ggctctgccc gagagcgtgg cacccgcaca
     3001 gagcggttcc gcctcggaaa gcgcaaccac ggacccggct tccgcgccgg cagaataact
     3061 gcgaaaacac cgcctatcct gcgatgggcg gtgtttttgt gacaaggcgt atttttaagg
     3121 aaatcgtaag ggttttcccc tgccggaatt tataatcggg cggtatcata caagcaacgg
     3181 gaggatgcgg atatggaaca gacaaaaatt cttgtcgtgg acgacgaccg tgagatcgta
     3241 ggagccattg cggcgctgct ggagctggag ggctacaccg tgctacgcgc ctacgacgga
     3301 ctggaggcat tggacgcgct gcacacccac gatgtgcagc tgatgatttt ggacgtgatg
     3361 atgccgcgca tggacggcct ttccgccacc atgcagatcc gcaaggaaaa gaacgtgccc
     3421 atcctgctgc tgagcgccaa gaccgaggac gccgataaga tcgcggggct ggccatcggc
     3481 gcggacgatt acatcaccaa gccctacaat cccatggagc tggtggcccg cgtgaaagca
     3541 atgctgcgcc gctatctggc gctgggcacg ctggcgggcg cgcccgcccg ggaaaatgtg
     3601 gtgacgctgg gcggcctttc gctggacctg gacgaaaagc agctgaccgt ggacggcgcg
     3661 cccgtgcgtc tgacggccac ggaataccgc atcgtagcgc tgctgatgca gaaccccggc
     3721 cgcgtttaca acgcggagga gatctacgaa cgggtgtgga acgagcctgg cttcagcgtg
     3781 gaaaacaccg tgatggtaca catccgccgc atccgcgaaa aaatcgagat caacccgaaa
     3841 aatcccaaat atctgaaggt ggtgtgggga cttggataca agatggaaaa ggcgcaataa
     3901 agcgtcgttt gggcagatgg cggcatttgc gctggtattg gcgacgaacc ctgtgctggc
     3961 atttgtgata tgcccgaatt tttccggcgc aatggggata ctgcttttgc tgctgagctg
     4021 tgcggtgtgc attggctgca cggtgttcct gttcgtgcgc accgggcgcg tacagggctc
     4081 ggatgacatt tttttaggag aatccggcgt gctgcccacc gaggtgctgg ctgcggtgat
     4141 cctgttcacg cctctgctgg gaatggcgct cgggctgtct ctgggcgctg tggatgtggt
     4201 catcgcgggc atcggcaccg cgggcggcgg cgtgatgctg ctgtgccttt tggcgttggc
     4261 gcgcaaatgg aaggccgggc gtctcatcgc cgacagcctg ctggcccgcg tctggccaac
     4321 gctttgccgg gcggcgcgct ttgccgggcg cgcgctgcgg agcatcctca cgggcgagaa
     4381 ccatctcaac gtccattatc ccgccgggcg tgcgtgggtc tatcgtattt tgtgggtgtg
     4441 cggagtcatg gccgggcttg tggtgctttc ctatctcatt ctcagcaatc agcttctggg
     4501 cgtaggcaat gcgcgttggt tcggctcagg gcgctatacc gccttcgcca tgttcgccat
     4561 ttttgtggtg acggcaggcg ccggatatct tgtatggcgc tcttatcggg acgcacagag
     4621 cttcgacgca cttctgcgcc agcttgatat tgccgcaaag ggcgaccccg cgcctgtctg
     4681 cgttcccgag gacagcagcc tgtacgctgc ttccaaggcc gtatcggaca tcggcgccca
     4741 tctgcaggaa agcgtggctg cccagatgaa aagcgaacgc atgaagctgg acctcatcac
     4801 aaatgtctcc cacgacctga aaacgcccct cacaagcatc atcggctatc tggaccttct
     4861 ggaaaagcag gacgacctgc ccgccgggac acgggactat gtgctcatcc tgcggcagaa
     4921 gtccgaacgg ctggccacca ctgtggcgga cctgttcacg ctggccaagg ccaccagcgg
     4981 cagcgaagcg ctggccatcg aaccgctgga ccttgtaatg gccgtgcgcc agacattggg
     5041 tgatatgaac gactccatcc ggcatacagg cgttccggta aaggcgacaa tgccggagag
     5101 cgccatcgtg caggcggaca gcggcaagct gtaccgcgtg ctgcaaaaca tcctggacaa
     5161 tgcgctgcgc tattcgctgg tgggcacgcg catttatctg gaggtctccc ggggcgacat
     5221 cgccacccgc ctgacgctga cgaacaccgc gtcctacgaa atgaacttcg ccccggacga
     5281 cgtgctccag cgctttgtgc ggggagacgc cagccgcann nnnnnnnnct ggaggtcggc
     5341 gtacagggcg acacgttcac cgtgacgctc acctttccgg gcagcgcggc cgcgggaaat
     5401 ggcgccgcgc ctgccgtcag gatggaggtg ttcggaacag cggagacctg cggcgaaaac
     5461 gacggcggag atacggagca tgcggcggag ccggatgcac tgccctgtac gcccgcagac
     5521 gtatccggtg cggatacgaa caaggcccgc ggggcgtgcg ccgcagcgcc ggaaacaccg
     5581 gaaccgtgcg gcgtctccac cggcatcccg gacaccgcgc ctgtgccaaa agcacccgca
     5641 ggcacgtccg gcgcagtgga aagcgcacac gcctccggca ggccggacgg tacggacggc
     5701 tgttccccgt acggcgaagc tgcggccttt tccgataaag cggccgcagc cgccgcttct
     5761 ccgtctgaaa cagccgcgca ggcggaacca aaagagccgg cgcctgcgca aagcgtgtcg
     5821 ccttctaaaa cagaggaaaa cagcggcgcc ccctcctccg tttcccctgc cgggcggcgg
     5881 aacggtcggc gtaaaaagca ttgagccgcc gaatacgaag cacaagacgc aaaagcggag
     5941 ccgcatatcc tgacaggnnn nnnnnnncgc ttggtctatc cggtttcgga attttgcttc
     6001 ccgcagggcc cttacgctac gcccgctttt gccagacggc ccgccagcag gcggcccaga
     6061 acacacgcaa gcacgccctt gcacacgtcc ggcacaatga acggcagcac gcaccagccc
     6121 agcgccgaaa caagatccgc cccggtgacg gccatgaacc acgcagtgcc gaacccgtaa
     6181 cagacgagca gccccgccag cattgcggcc ccgatcacag gcagcttgcc cgaacgtttg
     6241 acggccagcg ctgtggccag agcaatgaaa atatatccga tcacataacc gcccgtcttg
     6301 cccaaaagca ccgcagggcc gcccataaaa cccgcgaaca cggggattcc caccgcgccc
     6361 agaagcatgt atacccccat ggaggccgcg gcccatgcag gctcaagcag catgcccgcc
     6421 aaaaacgcgc cgaacaccgc aaggttgaac ggcaccgggc cgatgggaag ctggatctgc
     6481 gccagcacag cggtaagcgc cgcaaacagc gcggtcaaaa caaggtcctt tgtcgcaatt
     6541 ttccgtttca tgtcattccc tcttttccgc aatgccgcgc ggcgggcaca aggcatgtac
     6601 cggccgcgga acggcagtct catttttcac cgtgccgctg cggcccggct ttattgaaac
     6661 cggatgctga cttccccgaa cgaaagctcc tcctgcccgc cgccgggcag acgcaccagc
     6721 agatgcccgt cgtcggtgat ggcaagggca tgggccggcc ggcgttcgcc gttttgaatg
     6781 acgtccacgt cccggccggg caccaggttg cgcgcgcggt attccgccat gaaagacgta
     6841 tccggcagcg cggtgcaaag ggccagaagc tcatccgcaa tggccgcagc aatgcggcag
     6901 cgccccacgt gctcaccggg ggtgaaaatg gacgcagcga tctctgccag ttcctccggc
     6961 cagccgccct ccggcggaag cagattcagc ccgatgccca ccaccagata gtctacgccg
     7021 cccgtttcca cgtccgccgc cgcctccgtc aggataccgc agcacttttt gccccggtaa
     7081 aacaggtcgt tcacccactt gatgtccagc cgcttgcccg ccgcgcgctc cacggcacgg
     7141 cacaccgcca ccgccgcggc gctggtgaca agcaccgcgc tctgcatggg cagcgcactg
     7201 cgcagcacca ttgagaggta gagccccgtg cccggcggcg acgcaaagct gcgccccctc
     7261 cgcccgcggc ccgcggtctg ggaatccgcc accaccagcg tgccgtgcgg cgcgcccgcg
     7321 gcagccatct gctttgccgt gagattggtg gattccagct cctgtgccac cacgaggttg
     7381 agcgatttgt tccgcaaaaa cagccgcacg ccctgctcgg acagcacctc gctgtccgta
     7441 tccagcgtat agcctctgtt ctgcgcggcg gcgatggcat gcccggcctt ttgcagatcc
     7501 ttgatggcct tccataccgc cgtacgggaa acgcccagct cttccgccag cacctcgccg
     7561 gagagcggcg tgccgcggtt ctcctccagc agcgccaaaa ttcgttcttt ggttgtcatt
     7621 gtccggtaca cttctttctg cagaaaaggt gtctggattt attataaacg aaaaaaacgc
     7681 attgtcaact catttttaat tctaagttga caatgcatct gattccttat tctcctgctt
     7741 ctccccgtcc acaggccgca gcgcaccata tgcaagccac agcaaaaaaa ccgctcctgc
     7801 aaacagcagg gtaacttgca gctgaaacgt gtccattgaa gcccaaacgc gccgcagcag
     7861 cgccagcgga tgcaatatat cccagctgtt cagccgcaaa aaacgcccga tgtacattcc
     7921 gaagccgcac agcaacagca ccgtcccgcc acaggcccat accgcgggcc gcggccaatg
     7981 ggcgcagcaa tatacacgga atacgcgcat ggcctccagg cccagcagca ccgccagcac
     8041 ggcgcccgtg cccagcagaa gcgtcagcag ccagtcggac acgttttcgc tgtggcgcac
     8101 cgtccaatcc gccgcgttta cccattccat attttgcggt gtatggatca gatccgtgag
     8161 catgtaaaat gtgttgggca aaaacagcag ccacagcaca gcgagcgccg cacgtcccgc
     8221 ccaaaggcgg caccgcccgg cagcgcaggc aaaacacagc ggcagcagcg ccaacagcat
     8281 attccatgcc agccccagcg ccaaaagacc gcctgttcgc caaacgtggg ccgcacaggc
     8341 ggcccagtac agtcccacgg cggcgcccca tcgaagtacg catcttttcc gcgccatgcg
     8401 tgtccctccc cgttcctgct cttgcagctt tgtaccgcaa agccataaaa cgtctatgcg
     8461 gaccgcccgg cccacacacc ctaaaggctg tttctatttt atcatacgcc tgtgcaccgg
     8521 acaagcagaa cgtttttcaa ttcgcggctg tcccccttaa tccacctccc aggttttgcc
     8581 tatggctgtc agttctgtgc gttcaaagca tgcgcgggcc tcccgcagga cagtctgcgc
     8641 ctgcggtgta tggtagtgcg tcaacagcag gcgctttgct ccgttttccg cagccagacg
     8701 tcccgccgtg gcggcgcgca ggtgatgtcc ctcttccgca cacggccctt gcgtcagcgt
     8761 cgcctcgcac aaaaacaggt ccgcgcccgc ggcgacgcgc gcaagctgcg gacacgcgcc
     8821 cgcatcgccc gaataggcca gtgtccttcc ctccgcctcc aaccgaacgc cataggattc
     8881 gaccagatgc accgtgccga taaactccag cgccgcgtct cccatccgga accgcagccc
     8941 gtcctcaata acatgcggct caaacacatc gttggtgcac agctcggccc gtgcccacgc
     9001 cggaaggcgc ggcgtgagca gaggtatcct ggggatggcc tcgccgcagg cccgccggga
     9061 ctcgagctga tatttcgcgc agaacaggtc tccgaaatga tcaaaatgca gatggctgat
     9121 ggcgatcagg tcaagcgcgg cgagatcgac gtctttctgc agcttggcaa ggctcccgtt
     9181 ccccatatcg agcaatatcc ttccgccgtc cgcctcgatc aaaaaacagg agcaagcgcc
     9241 gtccgcctcc gggcaggtgg cgttatttcc atatacggtc atacgcatgc gcagcctctc
     9301 ctttcatata tccacgtaga taacttcttc cagtgccgcc aattgcgcgc ccaggcgctc
     9361 ctgcgcaagg ccgggcggca gccgcacaaa aagctcgtaa tgcacgccgc tgcgcttgtg
     9421 gctcgcctcc agatttatca cctgcacctg catctcatcc agagccgcca gcgcctgccg
     9481 caggccggac gggcccggct gcgtgcgcag gcacacagcc gtacatccgg gcgcgcgcgt
     9541 ccagcggtaa tcccggtgca gcatcagctg cgtaagaacg acgagcgccg tggaaaaaag
     9601 agacaccgcg taaagcccgc agcccaccgc catcccgatg cctgccgtcg tccagatgcc
     9661 cgcagccgtt gtaaggccgg taagcgtcct gtgccgcatg aatatcattc ccgcgcccag
     9721 aaagccgacc ccgctcacga tctgggccgc aatacgcgtg ggatctgcct tgatgccctc
     9781 ggcaaacagt gcaaggtcaa aaaaaccgta tttggaaacg atggtgataa gcgccgcgcc
     9841 caacgctacg atcagatgcg tgcgtacgcc cgcttccttc aggcgggctt tgcgctcaaa
     9901 gccgatcaca agcccgcaca gcgcggccag aaaaatacgc agcaggcatt cccaaaaaag
     9961 tgtcgcaggc atatttccgg taaaagacaa tccattcagc tcctctcaca gacgaaaagg
    10021 ctcccgttgc ccggcacatg ccggaaatgg gagccttgta tatcttcccg ccgccggaac
    10081 gagtacagat gaaccattct caccggtcga acagaacgcc gcatatctgt cccggctgca
    10141 aagccagcag cagagcatcc ccgtcccgcc gtacaccggg cgcatccccg tccggggctt
    10201 tcactgtaca ggctccgtcc gcagccgtgc cgtgcaccgt cagcatgcag ggcgcgccgg
    10261 tgtcattggc cgccagcacc attccgcgcc cgtccgcaag ctgccagcag gccgcatagc
    10321 agaagtccgg cacaggcgcc agccaggcgt cgtccaaaaa gcgcgcttcc ttgagcagcg
    10381 gctgcagttc cctgcggaac cgcgccatct tcctgccata gatatgatat tcgccgtcct
    10441 gcggacgcat gcgggtggta atgcccatcc acagcacgct gcccaatgta acggcacggt
    10501 gcatatcgcg gtagaaccac aggcgctggt cgcgatcttc cgtctcccag ccgcgggggt
    10561 tcaccatatt cacctgcacg aattccggga acgtatattt gaacacattg taatattcat
    10621 catattccgc gccgttccag gtcaggttgc cccaggtata gctgccatag atatcgccgc
    10681 agttctctgt catgatatac gcattggggt tgtgctttcg cagacggcgc agcagctcgc
    10741 gcagcacata cacatagccg ttgttgaatt caccgatatt ttcgtggctg tgttccgcac
    10801 agtagcatgc gaaaggctct gcagaagcca gctggtccag gtaaatgccg tcacagccgt
    10861 aggctttgac gcagaattcc gcagtgtcca gcaggtaatc ccgccagagt gtgtcggacg
    10921 ggcagttcac cgtgaaatgc tccggcccgt aggtctcgcg gtacggctcg cccttttcgt
    10981 tgcggacggc cattttttcg cccacggtct tgtggaaatc cgacttcacg tcaaaaatcc
    11041 gcgcgttgat gtacagcgtg gaaaacccgc cgtgctcccg cacgtattcc agcccgcgcc
    11101 ggaattccat cgcgcttccc agctccatgt ccggataata ttccgggtag aacgagtcga
    11161 agcccgtacg gttccagctt gccagaaaca tgtggcgcac gccccaggcc gcgccctctt
    11221 cgtacatctg cgggatatca cggaacgtat gctcgatatt gcctgtgcgc ttgaaattgt
    11281 agcattggtt gagcgcgcat tcgtccgcca aaaacgccgg attgtggtcg aaatccaaat
    11341 agggcgcgat atacgcgcgg taaagctgcg cgccgtaatg ccagtccttc gtggtgacgg
    11401 ccagaatata ttcccctgtt tcatag
//
LOCUS       DAWBSB010000056        11392 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1334_length_11392_cov_3.030520, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000056 DAWBSB010000000
VERSION     DAWBSB010000056.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 11392)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11392)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11392
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1334_length_11392_cov_3.030520"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3408..3417
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3878..3887
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7376..7385
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2057 a         3737 c         3171 g         2397 t
ORIGIN      
        1 gccaaagcag taaaacgact gcgtctcggg atatacgaca aaggacggcg ttttttcatt
       61 gtgaaacggg cacagcccgg tatgggtgcg gccgcggtgc cgcagctgta catagctctg
      121 caccacgtcc acgatatcgc tgcgctgcac cagttctgtg atataatcgc gcgggatcat
      181 cctgccgccc ccttccgtta tctcctgcgc cgcgccggcg ccattgcatt aatatttcac
      241 gtcttcgccg ccatgacccg tgtacgatga aaccacaggc ctgccggacg catccagcag
      301 cggcgtaagc cctgcgccgt agccctcgcc catgaacagg tactccactc ctgtctccct
      361 gtcccgcagg atacggaaat aggcgccaaa gccgctgccc tgcttataga tcacttcaaa
      421 ccgtttttcg tccataacgc tcctttcccc gcatcacagt atctgccact tctgcggcac
      481 gaacagcgat tcgaacgtgc tggtggcgta ttcgtcgctc ataccggaga tataatccgt
      541 cacggcgcgg tccacgccct cctgccacat gatatacagg aaagcctgcg gcatatgcgt
      601 gggcgtatcc ttaaaatatg cgtacagctc acggatgagc ttgtccactt tctgctcttc
      661 ctttttcgcc accttgtcca catacacggt gctgtacata aagtcgtgca gcgcctcgta
      721 agcggcggcc cgctcggcat ccattccgat atcttcggcc ccgtgctcca cgatggccat
      781 gatcagcgag gtgatccgtt cagatttcgt gctccccagc acggaggtga tctccgccgg
      841 gatatcctcg ttgcgcagaa cgcccgccgt caccgcgtcc tcgatatcgt ggttcatata
      901 ggcgatatga tccgcaaagc ggacaacgcg cccctccctc gtagcggccc actcaccgcg
      961 tgtgtgagtg acgatgccgt tgagcacctc ccacgtcagg ttgaggccgc tgccatcctt
     1021 ttccagcttc gtcaccacgc gcacactctg ctcgtagtgc ttgaagccgc cggggcagag
     1081 ccggttcagc gcgcgctcac ccgcatggcc gaaaggcgtg tggcccagat catgcgccag
     1141 tgcaatggct tcggtgaggt cctcgttcag gcgcagggcc cgcgcaatgg tccgcgcgat
     1201 ctggctcact tccagcgtgt gcgtcaaacg cgtgcggtaa tggtcgccct ccggcgaaag
     1261 gaacacctgc gtcttctgct tcagcctgcg gaatgccttg caatggatga ttcggtcccg
     1321 gtcccgctga aagcagggcc gcagcgggca tggtttttcg ggtacggccc gcccccggct
     1381 gttctcgctc aatgttgcaa acggagacag cgttatccgc tcaatctcct ccgtgcgctc
     1441 ccgtaccgtc atcagccagc ccccttttcc gcacagcgtg ccccacatgc tttcggccgc
     1501 cgctgtcttt catggttcca aaaattcggc gtcatataca ctatacgaca aaggctccgg
     1561 tttcccttta aaattttaag aaaattttca gaaaggggca aaatagggtt tggaaaacat
     1621 aatatccgcg ctgccccggc aaagctcctg caatttcggc aaaagctgcg tttcaccgcc
     1681 tgccagcatc acaaagggca gcgttaccct gtcggcgaac tgcacctcct ccagccgggc
     1741 gcctgcgtcg gccagcagcc gctgcgccag ctcatacaat ggatattcca cccgcaaaac
     1801 gccccgcacg cacacctgta ccgtcacctg ctttgccgcc gccagaacgg cgtttgccgc
     1861 cgctgtatag gcgcgcacca gcccgccagt gcccaacagc gtaccgccga aatagcgcgt
     1921 cgtcacaacg atgcagtcca caaggcccgc gtggcggatg gcctccagcg tgggcaggcc
     1981 ggaggtcttg gccggctccc cgtcgtcgga ataacgcatg cgcgcgccgt cccgcagaat
     2041 gtatgcgtaa acattgtggt tggccgtacg gtgctttgcc cgtatcccgt tcaaaaaagc
     2101 cagcgccttt tcctctgtgt ccgcaaaggc cgcgtgggcg ataaattcag attttttctc
     2161 ctcgatgcgc gcctgcgccg tgccctgtat cgtcttatat gcttccatca tccgccccct
     2221 ccagccgccg caggagcact tgcgcgtccc gccgtttttt cagtgtgacg cacctcacct
     2281 ccgggtgcgc agccagcagc gccttcacat gtgtgccgtc ccgcttcggg aagtcccaca
     2341 cccagcgcag aaagctcagg tcgaaccgct ccgggcaccc cgcgcccata tccgggcgca
     2401 cgcggccgtg cgttgtccat acgcgccgca gcacgcccgc caggcacacg gcccgggaat
     2461 agtccaaata aaccgcggcc tgcgcgcggg agagccgcag ttccagcgag cttccgaagt
     2521 tcccgtcgat gatccacgca tccttcgcga tctcgttttc caccgtgcgc cgccacgccg
     2581 cacgctccat ggccacccag ccgggctgcc agtacaagcc gtccagatgc accaccggaa
     2641 ggcccgtggc cgcccccaaa cggcgcgcca gcgtgctttt gcccgcgccg ccgcagccca
     2701 ggatcaggat tcgtttcgct tcgcccaatt ccattctttt tctcccaaac agcaacaaaa
     2761 caaagggcgg ttcgcttgaa accgcccttt gtaaggaacc tattattcgc ccagattgaa
     2821 acgcttttta aagcggtcca cgcgtccgcc ggtatcaacc agcttctgct tgccggtgta
     2881 gaacggatgg cacttgttgc aaacctcgac gtggatctcc ttcttcgtgg agcgggtcgt
     2941 gaacgtgttg ccgcaggcac aggtgatcac cgcttcctcg tacttcggat ggatgccctc
     3001 tttcacttgt attcacctct ttctggttta tgacattggg ggccatgctt aatacatgcg
     3061 cccatcacaa ccttcaatag gaggctacca gcgtgcgcca tcctgtgcgc acggagcagc
     3121 catcggaacg attgccctca tattttacca cgttcccgag ggaaatgcaa gcggtatttg
     3181 tccgcttatt tcagaagcgg ctccacagcg gcggccagcg ctttttgctg tgcctcggcc
     3241 tcggcaaggt ctttgcccag cgtggtaaag taggtcttga tcttcggttc ggtacccgag
     3301 gggcgcacca ctacactggc gccgcccgcc agtgtgtaga tgagcacgtt ggccgccggc
     3361 agctcgatgg gctccgtttt gcccgtggct acgtccgttt tggtgcgnnn nnnnnnnccg
     3421 ttacgacctt gtatcccgca aattccgccg gcgggttctc ccgcagcttt gtcatgatgc
     3481 ccgccatggt ctccatgccg gacaggcccg ggaattcata gctgttcact ttgttcaggt
     3541 aacggccata ctcggcgtaa atgcgctcca gctcctcctt gatggacgag ccgatgctgc
     3601 ggtaataggc cgccatttcg cagatgagca tggagcctac gacggcatcc ttgtcacgta
     3661 catagctgcc cgccaggtag ccgtagctct cctcaaagcc gaagataaag cgctccacct
     3721 cgcctgcggc ctccagcccg gcgatctggt cgccgatcca tttgaagccc gtaagcacat
     3781 ttcggcattc taccccgtaa tgcgcggcca caacgtccgc cagcggcgtg gagacgatgg
     3841 atttaacaca caccggggct ttgggcatgg tgccgttnnn nnnnnnnctc ggtgcggccc
     3901 ttgcagatat aatccagcag cagcacgccc acctcgttgc cggaaagcag ctgatagctg
     3961 ccgtccttac agcgcacggc aatgcccacg cggtcggcgt ccgggtcggt ggcaagcatc
     4021 agatccgcgc cggatttttc cgccagcgca aggcccaggg ccagcgcctc acgaatctcg
     4081 gggttgggat acgggcaagt ggggaaatta ccgtccggat actgctgctc gggcacaatg
     4141 gtgatatccg taatgccgat atcccccagc acgcgggtga caggcacgag gcccgaaccg
     4201 ttcagcggcg aatacaccag cttcagcccc gcggttttgc acaaacccgg gcgaacgctc
     4261 tgggccttga tgttctcata cagcgcctcg atggtgtcct ggccgatgta ctcgatgccg
     4321 ccctgctcca gtgccgcgtc gaaatccgcc agcctgatgc ccgcgaaaat atcggtggcc
     4381 tgcatctcgg catagaccgc gtcggcggct tcgctggtca tctggcagcc gtccgggccg
     4441 taggccttat agccgttgta tttggccggg ttgtggctgg ccgtcaccat gatgccggcg
     4501 tcgcagtgca gatagcgcgt ggcaaagctg agcgccggaa cgggcatcag ctccttgtaa
     4561 agatacgcct tgacgccgtt gcctgccagc acgcgcgcgg cctcccgcgc aaacacgtcg
     4621 cttttgatgc ggctgtcgta ggagatggca acgctttttt tgccgccctg cttgttcaga
     4681 taatttgcca aaccctgcgt ggccttacgc accatataga tgttcatgcg gttcgtgccc
     4741 gcacccagca cgccgcgcag gcccgcagtg ccgaactcca actccaccgc gaaacggtcc
     4801 tgtatctctg cgtcaatgcc ctgaatgctg agcagctcgg gcttcaggtc cttatcctcc
     4861 agttcggcgg caagccaccg ttcatattcc tgctgataca tcaatatgcc cccgctttcc
     4921 gggaaagctg cgcttcccct tttcagtctc aggcggccga agcgccgcgc ggcgtgccgc
     4981 cttttatact tcttatcata agccatgggc ctgggcctgt caattcgcac ttgtacaaaa
     5041 atgacgcgca agttatacta aaaacacacc catatgggcg taccggcgcg aagtgcggcg
     5101 gaaaacggcg aaaggcggcg gaaggatgtt tgcaaggatc aacagcctgg gcgtatatgc
     5161 gctgaatggc ttcagtgtga gcgtggaggc ggatatcagc ggcggactgc ctcaattcac
     5221 catcgtgggc ctgccggaca gcgctgtgcg cgaggccacg gaccgcgtgc gcagcgcgct
     5281 gaaaaacctg tccttcgtct ggcccgtcag ccggatcacc gtcaatctcg ctcccggcga
     5341 tatccgcaag accggccctg tgtacgacct gcctgttctt cttgccattc tggctgcctc
     5401 cgggcagctg cccgtccccg gcccggagga cgccttcatc ggcgagcttt cgctggacgg
     5461 cggcgtgcgc agtgtgacgg gcgtgctgcc catggcgctg gccgcggtgc agcagggcgt
     5521 gcgccgcctg ttcgttcccg ccgaaaacgc ggccgaggcc gcggcagccg gcgcgctttg
     5581 tgtttaccct gtccatacgg cgctggacgc agtgcgccat ttgacgggcg aagcgcccat
     5641 agaaccggca cagcccgcgg cctttgcacc cggcgggccc cgggacgtac cggactttgc
     5701 agatgtccgg ggccagctgg aggcccggcg cgccatggag atcgccgccg ccggcggcca
     5761 caatatttta ttgatcggcg cgccgggcac gggaaaatcc atgctggcca aacgtctgcc
     5821 gggcattctg ccgccgctct cccgcacgga ggctgtggag acgacgaagg tatattctgt
     5881 ggcgggcatg ctgccccgcg gcagcgggct tgtgagccac cgcccgttcc gcgcgccgca
     5941 tcattcggta agtgcggcgg gccttgtggg cggcggcagc acgccgcgtc ccggcgaggt
     6001 gagcctcgca cacaacggcg tcctgttttt ggatgagctg cccgaatttc accgcgacgc
     6061 tctggagatc ctgcgccagc cgctggagga cggcacggtc accgtcagcc gcgtggcggg
     6121 cagcgcgtca ttccccagca gcatcatgct ggccgccgcc atgaatccct gcccatgcgg
     6181 ctattttggg caccccaccc gcgcgtgcag ctgcacgccc tatgccattg agcgctatct
     6241 gcagcgcgtt tccggtccgc tgctggatcg gatcgacctt catgtagagg tcgcgcccgt
     6301 ggaatacgcc gaccttgccg gagattcgag cggcgagagc agcgcagcga tcctgcggcg
     6361 cgtgctggca gcccgcgccg tgcaggacac ccgctataaa ggtacgggcg tcacctgcaa
     6421 tgcgcgcctt cccagcgcga tgctgcgccg cgtatgccgc cttgcgccgg acgccgaccg
     6481 tgtgctgcgc ggcgcctttg agcgcatggg cctttccgcc cgcgcttacg acagggtgct
     6541 gaaggtgagc cgcaccatcg cggaccttgc gggcagcgaa cgcatcgaag cagagcatgt
     6601 gcgcgaggcc gtgcagtacc ggaatctgga ccgcaaatac tggtatacgg cgtaaaacat
     6661 ccgcaggagg caatgtatat gatcggactc gcattttttc tcatcggcgc cgccattgcg
     6721 gcaggcttca ttgtgctggg ctggaaaatg gcctgcaaag cgcaggagca ggatgaaaag
     6781 aaaaacccaa aatgacctca ggccgttttc gaaagcggcc tgcccgggcc gagcgttata
     6841 gacatatgaa aaaatcaaag cggcggaacg catctcctcg ttccgccgct ttgattttgc
     6901 ttcattactt ctgctgctcg gccacgatca tcacatgcgt agcgctctga tatgccagca
     6961 gaatcacatc attcgggtcg tcctcgctcc ggagcgagaa acacacccag ctctcatcca
     7021 gcgtggggcg cgccgcctca atgagcgccg cgtcggcctg tgtggtagaa gcatccgcca
     7081 gctgcgcgtc gatcaccgcg tcaaaacgcg cctgcatatc cgccgcgggc gtctcccatt
     7141 tggccaggat atccgccacg tctttttcat aggtcagctt cgcaaagctg taacctctgc
     7201 cgtctgcaat atgctccgca gactctttgg aaaaacccgc gggcagcgcg gcgttccagc
     7261 gctctaccat accgacgctc agcgggtcgt cgctggcctg tacgccggta ttgcggttca
     7321 ccagataaaa aatccccgcc agcaccagca acagcgccag gcataggtat gtcatnnnnn
     7381 nnnnnttttt tcattggtct ttttcacggc cgcccgcggc cgcccttctc cttttttatt
     7441 ctttccggcc accggccggc tcatccattg ccgcccatgc cgccaaaagt gaagcgaaag
     7501 gtttcgccct tctccagcgc cacatggtat tcggcatggg gtccggcgcc ccaggtgttg
     7561 tcgcccgcaa cgcccagctg cccttttgca cagcgtacca cggttttgac ctttggcggc
     7621 agctcgtact ggcggcgggc gttctccact tcgtgcggag tatacggcag cgcactgagc
     7681 ataaagccat tcccggcaag gcacagcccg aagcccgcgg cgtcggtaac ggcggcagcc
     7741 tgcacgcctg tgcgcgcgcc gctctcctgc gggttgaaat aaggctgcag cgccgtgtgt
     7801 gcgtcaaagc cgaacagccc catgtgtgcg ccgctggtaa aatccgaata cgtctcgccc
     7861 ggtcccatac cgtaataggc aacgttgcgg tactcggcag gcagtgtcag catcaggccg
     7921 aattccggca cgctctccac ggcctttcct ttccatttca gcgcagcctc cacctttcca
     7981 tcccctgtga tggtataggt gacatttgcc cgcgcgccgc cgggaagcgc atactttgcg
     8041 tggatgcgca cggacgtccc gtccgtctct gcctttgccc tgcgcagttt tgcatacagc
     8101 ccggccgttt tgaagggcag gtgcgcaagc tccatacccg cggcggtgtc gttgtcggtc
     8161 ggcgcgcgcc agaagttgag cgcaatgggg cgctgcagca gttcgcggcc tttccagcga
     8221 tacgagataa cgctgccctg agcgttcttc gtaaacagca ggctgaaatc tctgccgtga
     8281 acgccgatat tgcagtcgcc ctccaccagc gtgaccggca gcgtacaggg cttttccggc
     8341 gccttccggg aaatatgcca ctgcccgaag gccacacaat ggcccttcgc ggcccaatgg
     8401 gcattctccc gcaggcacag cgacgcatcc acggtatact cgcccggcgc ctgcggaacg
     8461 gcaaagggca gctccgcgcg cgccgtctcg ccgggaacag cctgcaggct gcattcgccg
     8521 cttttcaccc gcgcgccatt cttcgtcagc tccaccagca gcacatagtc cgccgtatcc
     8581 gtgaacaggg atttgttttt cacggtaacg cccgtctcgt cggacaggat atcgaaatcc
     8641 tggtagcagc ccttcgcctg ggcaagtttt ggcgtgggcg tgcggtcggc gaacagcagg
     8701 ccgtttccca cgaattcaaa atccgacggg cggtccccga agtctccgcc gcaggcgagg
     8761 cagcgttcgc ccaggcagtt gtccgtttca atcccctggt ccacccagtc ccagataaat
     8821 ccgccctgat attgagggat gacgtctgtc atacgggtgt aacggtccat cgcgccgcag
     8881 gaattcccca tggcatgcat gtattcgcac ataataaagg gcttttccgg atgtttttca
     8941 aggaaagcgg ccacatcagc ggccggggta tacatctggc tctccatatc gctggtatca
     9001 gggtaacgcc tgtcatggaa cactccctcg taatgcacca gccgggtggg gtccgtttcg
     9061 cggaaaaagc ggctcatttc gtagatgtcc ctgcctccga agctctcgtt gccgcagctc
     9121 cagatgagca cagaggcatg gtttttatcc cgttggagca tactgtttgc ccggtcaagc
     9181 acagcgccca gccactccgg cctgtcgccg ggaagcgcgc tctccgctcc gtccggccgt
     9241 tgccacgtgc cgtgcgtctc caggttcgtc tcgtcgatca cataaaggcc gtactcgtcg
     9301 cacaggtcat accattccgt gcggttggga taatggctgg tgcgcacggc gttgatattg
     9361 ttctgcttca tggcccgcac atcgctcacc atatcctcca tcgttacggc tcggcccgtg
     9421 cggcagttcc actcatggcg gttcacgccc ttgaacacga tgcgcctgcc gttgagcagc
     9481 atcaggccgt cccgcagctc aaacctgcgg aagccggcct ttccgcgcac ggtctccaga
     9541 agatggccgg ccgcgtcggt aagcgttacc tcataggtgt aaagctccgg cgcctcggcg
     9601 ctccacagac gcgggcgttc cacgggcagc tccaacaccg catgcccgcc cgtcacaggg
     9661 gccagcaccg tttttccgtc cagcgtcagc gtcgcctcgc ccacgcacat tccctccagc
     9721 ttcagctccg cacgcagcgt acccgcagca agatcttctg aaagctgcgg cttgacccac
     9781 aggtccgcca gatgggtctg cgctttcgtg aacagcgcca cactgcggaa aataccggac
     9841 atgcgccaga aatcctggtc ctccagccag cttgcggagg aaaagcggta tacgcgcact
     9901 gccaggcggt tttcgccgtc ccggtgcaca gctggcgtga ggtcgaattc cgcgggtgtg
     9961 aagctgtcct cactgtaacc gatgaacacg ccgttgcacc ataccgcaaa cgccgtttcc
    10021 acgccgtcga agcgcacgca aacagggccg tcccagcttt ggggcacagc aaaaccgcgc
    10081 acatagcatc ccacaggatt ataccgctcc ggcacggcag gaggctccag tctctcgtgg
    10141 ccgtcccagg gatattgggt attgacataa tggggcgtac cgtacttccc gccgccctga
    10201 agctggatat agcccggcac cgcgatttcg tcccagccgg aaacgtcata tccctccctg
    10261 taaaattcct gcggagaggc gcccggcgtc tgtgcataaa aaaacttcca cgcgccgtcc
    10321 agcgtctgct ccagcggcgc gccgtccgcg ccccgcacca tataagatgc atgagccggc
    10381 agacggttca cttgaaacac ctgcgggtct gtcagccatt tcgggtcgaa ttccatggga
    10441 tgcctccttg tgaaatttct tattcccggc cgccggggct cacacggcgc caaaagcgcc
    10501 gcccggcggt cgcatcgtgt gtttcacggc ctgtactgct gcgcgggccg atatttttat
    10561 tgtacctttt tccctgtgct gtggcaacgc ctgtgcaatg ttttttaatg tattattgtt
    10621 gcccgtctct tctttgcctg gcaaaaagcg ctcctgtctc ctgtgctgtg aagctctccc
    10681 gcgcgccttt cttgcatcgg cactgcggct gtgctatgat aatgaatacc ttatacccaa
    10741 acaactgtcc gaaatgggag cgtactatga cattacaaaa attgcgccgc tggctcggtt
    10801 ttccgctgga ggaccgatac cgcgtgcaca ttgaacagga tatcgtacaa agcagcctgc
    10861 gccccggcat tttcagcgca ctgctcatcc tcgcttttca agctgtgatg atggtgctct
    10921 cgctgctgcg caaaggtggg ccgttcgcct ctctccgccg acaaggatat tggtggctgt
    10981 acgtaacact gttttcggta acgctgctgt ttttgctgct gatcgtattt ctgatgcgcc
    11041 gccgcaggcc gtgcctggac acgttttttt taccgctgca gacattttac accgcttttt
    11101 tgtgtctgtg ggggacgtgc gtcacgcttt tggaccaatt cggcggcaac agcctgagcg
    11161 tgtttactta cgtcacgctt tctgccgcag ccctcaccgt gctgcagccc tggcagtccg
    11221 cgcttatctt taccgggaat tgcctgtttt taaacctgct gttgccctat acgcctgccg
    11281 gaccggataa tttttacagc aatgcggtca acagctgctt cgtcacactg ggcgcttttt
    11341 tcatctctct ttggttttac cgcagtaagg tctcgtcctg ttacgccaag at
//
LOCUS       DAWBSB010000057        11336 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1342_length_11336_cov_3.678486, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000057 DAWBSB010000000
VERSION     DAWBSB010000057.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 11336)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11336)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11336
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1342_length_11336_cov_3.678486"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    8062..8071
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8613..8622
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2279 a         3623 c         2975 g         2439 t
ORIGIN      
        1 ccggcgctta ctcccggcac ataccgattg ggtctccagc tggaggatta tggccggggc
       61 tgcctgccgg aggacgcttg ggtgaccgcc tgcttcaccc tcacggaaaa tactgtcgag
      121 ccttcgcaga tgtgcgcata tccacggact tcgggggaat cctaactgtg gaggcgaaat
      181 attactatga gtatattaag cagtttatat cacggagaag tccacaacaa acccaacgat
      241 gtgcacaaca ctcccgaaaa aagcacgggg catacagacg gcagcgctgt gctgcccgct
      301 cacgcccaac ggcgcatgca agcgctgcgg aacggcaacc ttcgtccgcc gctgtactac
      361 acgcagcagg agcagacgcc ctgcgccaac ggcgtgtggg aatctccgca cacggtggcc
      421 agcaagctgg aaagcaagga agaattcccc cagggataat gcaaaaaggc tccgtttgga
      481 gccctctccg taaagaagta atcaatccca catctgcaga gatgacgagc atcggatttt
      541 gtccctaaaa attcacagaa aagcaccatc taaagtaaat tctttagatg gtgctttttg
      601 cgcccgcgca aggagggcag ttataaactg aaatacactt tttatatctg atttatgata
      661 aaatctacat tttcttcaac gggttttgtt ccgtcaattc ttaggatagg actacttaat
      721 agctgtatcc attcctcaac cgtactctct ggtctggatg taacaaactc aaaaaacttt
      781 tcttcctgtt catgcaaatc tccacctggc agcattctat tgccaaactt ctgaaaagag
      841 cgctttttaa cccgttgcac ccgaatgtcc tttggagtat caatcaatac agcatactga
      901 aaaaagggat aaatcgattc tccataatct ccttttacag cagcaaaaac aaaattttca
      961 tgtaccttaa tcgcatgaaa aagcattttt tcagcctcct cacgcgtgcg tggagaagca
     1021 tagatataat ttgggtctgt cttagggaaa aacagatctt cattatctat aaaataaaaa
     1081 tccaatttct cagcaagcgc ttttcccaat gtactctttc cagcaccgtt taagccgcat
     1141 atcacaattc ctgttcccac aaaactcacc gttaacccga ttttctgttt cgccccaatt
     1201 ctatggccct tcattttctg ccgtcgctcc gtttggctta cggcccgacg gcgttatgct
     1261 tcgtccctgc accaggcgat aagcggggca agcgcggccc tgtctgtaaa gtccacctgc
     1321 ctgtcatatg cggccaacag ccctttttct tccgtactgc gctcgctctc cggttttttc
     1381 agcagcattt ttttcagcat tgccatcatc gccttgtgta caggcctcat attccggtaa
     1441 tccatcccgc cgcgcaggtg gaaaaagcag atattctcca gcatttccgg cgtaaaggcc
     1501 cgggccgcat cctgcatgat ctttttcaca ttttccgggt cgctcgggtc cgacagcccc
     1561 actgtaaaca ttgccagacg tttcccctgc aatgcaccat acaactttgc catacgcgca
     1621 gcgccggcaa tgctccccgc atacaggccg ccgccaaata tcacgcattc atagttctgc
     1681 acaaggcgcg cgtccgcctg cctttcctcc gcagcatcgg catccagcgc ctgtgccagc
     1741 cattgagcat accggcgtgt actgccataa tgggaacggt ataccaccag cgttttcatt
     1801 ccaacccctc ctccaccgtt tgcatcttct ccaggttttc cagcatacgg gccagcactc
     1861 cggcagcctg ttcagcctcg tctgcacgga tgcctgtata cagcgcccgc ataaattcct
     1921 gctggcgtgc gccgtatttt tccggcagct ccgcgcactt tggggttcgg tgcacgcggt
     1981 tcttgcgtgc gtcccgcccg tcctgcgcca gagccacata tcccttttgc tgcagcttca
     2041 aaaccagctg cttcacgttt tgatgcgaac ttcccacacc gcgcgccagc tcgcccagtg
     2101 ttggcggagc tgaaaagttc tccagcaccg ccagcacaaa ccactgcttt gtcgtgatct
     2161 catcgtaaaa ccggtccccg gcggcttgca ggcggtttcc cattgccagc agcgagccaa
     2221 aaagcacata gggtgcggga taatttttca gctccatgca aagtctcctt ctgaatttat
     2281 ataatatatt acatttttat ataatatatt acacaaataa aaaattgtca agttccgcgc
     2341 gatttgtgct atactatgga cagagaggtg atttgtatgc cgttcattct gacggaaaac
     2401 cgtattgcat atgtgcagga gggccagctg ctggcagagg ccacttttcc tgaattcgcg
     2461 ccgggcgttg taaacattga ccacaccttc gtcgacgaag cgctgcgcgg gcagggtgtt
     2521 gccgcacaga tgatggaacg catcgcccgc tctttgcgcg aaacgggccg caaagccatc
     2581 cccacctgca gctatgcaaa aaaatggttc ggccagcatc cggaatatgc cgacgtgctt
     2641 cgctgaacgc tcatacccaa aacgcgcctt ccctgaaacg gacatccgct ttggggaaag
     2701 cgcgttttct gttttatttc tccggcagcc gcttcagagc ggcgcgaata tcccgttcca
     2761 gcaaagaggc catgctttcg gagtaacggg aaaggtccgc gcacaggagc gcctgtctgg
     2821 ctcgcggaaa gcagcccggg caggctgctg caaactgggg cagccgttga acgcaccagc
     2881 gtgcagcggc cgccttttcg tctgtgatgt gggcaagata ccgctccagc atctccatta
     2941 cctgttcagg gcccgcccac tgtgcgttgc atgccagcag gaccatgccc cgcatgcgta
     3001 cataagtgcg tttatcgtcc agcattgccg caaaagcatc ccaaaagaca cacaccgcgg
     3061 ccgaagcttc actttccgcc tccagcgtac acagggctgc atatgcggcg tcgttgttct
     3121 tccctgtcag ctttttcacc tgcgctgcaa tttgctcatg ctccattccg aagccttctc
     3181 ctttcttttt gcggtcagta caggcataag atccattttt cccaaaaacg gcgcagcggc
     3241 ccgctctctt tctcccgtcg ggccgcgcat tggccggcat attcggtcag cgccccaagc
     3301 tcctccggtg tcagcacatg attgctgaac tttgcttttt ccgccagttc cctcagacgc
     3361 gcgggcggcg gcgcagcgcc ccggcacagc cgttccagat agacccacgt atccagcgcc
     3421 gcccggctgc gggcagggcc ctgcatcctg cgcgccctgc gcgccaacac aaggcggcgg
     3481 cgcagccata cggcagccgc caatgcgggc agcgccagca gccacagcgc ctgcaccggc
     3541 agctgccacg aatccgcccc cgcggccgcc ggccccgacg tgtccggccg ggccccgccc
     3601 ggctcctctt cggagcgttc tgttcccggc gcgcggtccg ggccgttcac cgcagggtct
     3661 gctccctgtt cctttcgtcc ttccgtttcg gatgacgcag tgtcgcggga ggcggcattg
     3721 ctttccccgt tctccggcac gccttcaccg gaaagcaatg cctccggcag cacactggtt
     3781 tcgcgcgcaa aacccggcgt cgcttccaaa ggcagccagc cgaagccgtc cagataatat
     3841 tccacccatg catgggcggc gctgtccggc acatcggccc taccgccctg tacaacagcg
     3901 acataaccgc tcacataccg tgccgggata tccagcgcgc gcagcataac cgctgctgcc
     3961 gaggcaaaat gcacgcagta accctggcgg gactgcgtca gaaaatgcag cacaaagtct
     4021 tcatcccgcg gctggacagg cgtatccaga ctgtaccggg ccgcacttcc gatgtattgc
     4081 gccacctgcc gggctgtctc tgccgtgccg ccgtcggtgc ggatgcccgc cctcgccgcc
     4141 agcgcctgca ttgcctgcgc cgtatccgcg ggcagcgcgg tatattcccg atagacggcc
     4201 tctctataaa agcgctccgg ctgttccagc tccggcagca gcggcagttc caaatggatc
     4261 cgttgttccg tccattcctg ctcatccggc ggtacactcg ccagccaata ttcgccgtaa
     4321 aattccgttg tccagctctg cgtgtccaaa tcatgcgtca cactgccatc ggaaacatat
     4381 gtcatccccg gcacatccaa aggatagtac ggcagatagc acagatccgt accatctccg
     4441 acgcttgtca ccgtcatctg ctcgccttcg ccgcccagcg cccccaggtt cagagcgggc
     4501 aactgcagcg ggtgcggcgc aggctctgtg ccctccgccg caagctgcac cgcacccagg
     4561 ctgcaatcct tccatgcacg gcccgtataa tcaccggcgg acgcgccgcg cagataaaac
     4621 acaccgtcaa ttccgcattc cacccgcagc actgtacggc ctgtgtaaac acgggggcct
     4681 gctgtctcca gccgcagtcc gccgccggag gatgcacgcc acggcaatgc agacggcgta
     4741 tttccctgca ccagagcgat cacgtcccgt tgcatcctgc ccgcccagga cggccggata
     4801 aagtctctgt ccggcagaaa aatctgcagc cccagcagca gcgccagagc gggcgccaac
     4861 aggccccagg taacacggcc ggcctgcgcc gggctggagc gccgcagcgc atgtgtcagc
     4921 agcagcagcg tccaaaacaa caggcacatc agctgcggaa cgggcgcggc agcctgcgtt
     4981 actaccagcg gcaggcaaaa cggcggcact gtcagcgcgg ccgaaaacaa agccgaacgc
     5041 tgccacagca gagccgcgcc cagcaacccc gccaaaatcg ccgcataagc acaaaaaaat
     5101 attgtcaggg aacggttttc caacgtcatt ggcacatcgg aaaaccacac ggggaccgcc
     5161 ggaaaatatg catggtacgc ctgtgtgata ttctgtgcag tcaaaaccgc accgcgcagg
     5221 gcgttttccc ctgcggacgc caggagcgcc gctcccagca gcagcgcagg aaggcgcagc
     5281 aggcgcgccg cacgcagcgg cagcgccaaa aaccatacaa cagccggaac cgccgcacag
     5341 ctcagcgcta ccgctccgcg cccaaccggc aggtcaaagc ctgtgacaac ggcaaatacc
     5401 gttcctccca cgcccagcca caacaacaaa aggctggaaa gcgcgctcca caacggcgct
     5461 tttcttggag cgttgtgccg gtctgtcctg cgccaaaagg gcgtcccggc ttttccggcg
     5521 ccggcgtgcc ccggggcaag gcgggtatgc cgccccgcag cagaaagcga aataccgggc
     5581 gtctttcgcc gtttatcccg catcatccgc cgcctccttt tccgcacgca gggtatagct
     5641 ccattccgcc tgcggcggcg cccaacccgg cgcccagccg tccgcaccgt ccgccggttc
     5701 agccaaaaga cggtacagca gcgcatccaa ctgcccggcc tcatccacca gcgccgtgtg
     5761 caccacgccg ttttcaccgg gccacaacac agtgtgagac actccctgct gcgtcaggcg
     5821 cgcggacaac cagcacaaat gctccaatac ccggtccagc gcgtcagggc cgccccgcct
     5881 ctccaccagc agaagggcgc gcgcccggca gggcaccagc gcctcccgga cgatcatctc
     5941 ccctgttttt gccgtcagct tccagtgaac cgtgcgcata gggtctcccg ggcggtaggg
     6001 ccgcagctca tgctcctcgg catacgcgcc cacccggggc ttcatcggtc cgccggatac
     6061 ggcccccggc agcggcggct ccggcgaaag ggcaacgggc cgcggccgca cggtgacaat
     6121 gaccgcgccc ggcctgcgca caggcaggcg gaacaggccc agataatcat atacccacgc
     6181 acgccgcacg ctgcaccgca gtgccgcgca gtgcgcagtg tccagctccc attccgcagg
     6241 agcctccccg cgcaagtgaa gccaccgcgc acagtcctgg cccggatgcg tccggtcctc
     6301 caccgaaata cgtacttcca tacgcgcggc aggcatccat cccagcatat atacctgtaa
     6361 aacacaggtt ttttcccgct ctaccgccgc gggcgcgtgc agcacgatac ccgctgtcag
     6421 catcgccgga aggcttacca caagggaaaa caaaggcagg cagacggcca gcatcagaac
     6481 ataccatgcc aattggccga aataaaagca ataaaacagc acggccccgc ccagcgccaa
     6541 cccatagacc gcgcgcgccg cccacaccct catttgagct gcggcgcgct tatactgcgc
     6601 atcagatcgt ccagcagcgc gtccacatcc cttgtctgcg gcgcggggat caaccggtgt
     6661 gccaccgtac agtgaaagat gcgccgcaca tccgcaggtg tgacataatc cgcgccccgg
     6721 gcaagcgccg cggcctttgc catagcggcc acagccagtg tggcgcgcgg gctcgccccc
     6781 tgtaccagtt ccggcaaggt ccttgttccc gccacgagcc gtacaatata gtccagtact
     6841 tcgtcgctca catagacctg gtctgtctgt gcgcgcagcg ccgccagccc ggccttgtcc
     6901 agagcctgct gcacgccatc caggggattg cccttctgtt tccggcgcag catgcgcagc
     6961 tcatcctgcg gttccgggta tccgagggac aacttcacca taaaccggtc catctgggaa
     7021 tccggcaaaa gctgcgtccc cgacgctccg gcgggattct gtgttgcaat aacgataaaa
     7081 gggcggggca gcgcatgcgt tacgccgtcc acggtcactt gcccctcctc catggcctcc
     7141 aacagtgcgg cctgtgtgcg gctggtcgca cggttcagct catcggccag gaacaggttg
     7201 cacagcacgg cgccgggctg gtattccatt ttccccacag ctttgttgta aagagagaaa
     7261 cccgtcacat ctgagggcag tacgtccggc gtgaactgca tacggttata gcgcaggccc
     7321 agcgccttgg aaaaagccag cgccatcgtt gtcttaccca cgcccggcac atcctccagc
     7381 aaaatatggc cgcccgcaag gatggccagc accgcacgca gcagcaccgc atccttaccc
     7441 accacagcct tttttacttc gtccattact ttgccgatgc ccgccatatc cgcacttccc
     7501 cgctcccgat tttctacatt ttatcataaa acgacagccc ccgccaccgc aggggccatt
     7561 gctgcctgtc caaacatgta aaagttttcg caccgcagcg cgcaaaggcc aaatattgca
     7621 ggatggtaaa ggctgtatat ccgctgtgca gcaatatttg aaaaataacc gcaggctatt
     7681 tttcaggcca tgcgggccgt ccccggcccg cggccatcgg gataagcttt tttattgcgg
     7741 ggcaataaaa aatggcctgc ggccgctccg gcccatatgg ccgtcaggac ggggctttta
     7801 ttgcgcagtc ataaccacca gctaagctgg tggcttgagt aggctctata agagcttatt
     7861 accgacaagc gtcttaagac gcactgaaat ttatttcgct tgcataactt gccccacagc
     7921 ccgtaaatgg gcgattgctg ttctaacgga agctttctat tattgaaagc tttctgttag
     7981 cagctcgctt cgctcgatgc ttgaagctaa agctttttac gggacacctc caccggcaga
     8041 gccggtggtt tccgtaatca annnnnnnnn nccccgtgcc gatttcctgc acgggggatg
     8101 ttttaaacag aacttaaacc agttcgatga tcgccatttc ggcggcgtcg ccgcggcgca
     8161 caccagtctt gatgatacgg gtgtaaccgc cgtcgcggtc ggcgtatttc gggccgatct
     8221 cgtcgatgac cttctttgcc acgctctctt tcgtgatgaa gccgtacacc tgacgcttcg
     8281 catgcaggtc ttcacgcttg ccgagggtca ccatcttctc cgccatcgcg cgcacttcct
     8341 ttgcacgcgt cgccgtggtc tcgatcttgc ctgtctcaaa caggaaagtc accatagcgc
     8401 ggagcatcgc cttgcggtgg tcgctggttc tgccaagttt tcttgtaccg ggcatcccat
     8461 ttcactcctt tgcagtttac tcgtcgtctt ttgtaagggt aaagccgagg gaatccagtt
     8521 tcgccataac ttcctccagg ctcttgcggc cgaggttgcg cactttcatc atctcgtctt
     8581 cggatttgtt gatcaggtct tccacggtat tgnnnnnnnn nngtattgat gccggcgcgc
     8641 ttcaggcagt tgaaggaacg cacggaaagg tccagctcct caatggtcat ctcgagagcc
     8701 ttttccttgc ccttgtcgtc cttttccacc atgatctcgg cgtccatcgc ctcctgcgaa
     8761 agcgtgacga acaggttcag atgctcggtc atgatgcgcg cggcaaggct cacggcctcc
     8821 ttcgcgctga tcgtgccgtc cgtccagacc tcaagcgtca gcttgtcgta gtcggtgatc
     8881 tggcccacgc gggtgttctc caccgtgtaa ttcaccttga gtacaggggt atagatgctg
     8941 tcgacaggca gagcgttcag ggaagcctgc tccgcagcct gcttgttgcg ttccgccggg
     9001 acataaccgc ggcccttgtc gaacacaagc tccatgatga gcttcgcgcc gtccgccagg
     9061 gtcgcaatgt gccaatcagg gttcagcacc tcgatctccg catcctgctg gatgctgccg
     9121 gcagttacct cgccctcacc gctcgcttcg atgcgaacgg ttttcggggt atcggaataa
     9181 agctttgcgg tgatgccttt gacgttcagg acgatctcgg taacgtcttc cttcacgccc
     9241 tcgatcgtgg agaactcatg gactaccccg tcgattttaa tgctggtgac tgccacgccc
     9301 ggcaggctgg aaagcagcac acgccgcagg ctgttgccca aggtggtacc gaaaccgcgc
     9361 tccagcggct ccacgacgaa tttgccgtaa cggccgtctg cggaaaggct ggcggtatcg
     9421 atcttgggtc tctcaatctc catcattcta aaaccctcct tagttcaggc aggcggcggc
     9481 acgccgcctg ccaaaatgtt cgcatacggt gatgaggatc acggggatta tttggagtac
     9541 agctcgacga tgaggtgctc ctcgatgtcg aaatcgatgt cggcacgctc gggcagcttc
     9601 gtgacgttga cagtcatctt ctcagcgtcc ttgtccagcc acgcaggcac ggggcgggcg
     9661 ctgttcttct cgatgcactc tttgatcttg tcgcagtccg tggtgcggac ggcgaccttg
     9721 tcgccctctt tcacgaggta ggaggggatg ttcacggtct ggccgttcac ggtaaagtga
     9781 cggtgcagca ccagctggcg ggcttccttg cggctcatcg caaaacccgc gcggtagacg
     9841 atattgtcca gtctgctttc aaggatccta agcagattct caccggtgat gccggatttg
     9901 cgctcggcca tttcaaagta atgggcgaac tggctctcca gaacaccgta atagcgtttt
     9961 gctttctgtt tcgcgcgcag ctgcaggccg tattcagaga ccttcttacg ggcctggttc
    10021 tggccgtgct ggcccggcac gaagctgcgg cgggcgagcg cgcatttgtc ggagtagcag
    10081 cggtccccct tcaggaagag cttctggttc tcacgacggc actggcggca aactgcgcct
    10141 gtatatcttg ccattgtttt tcctccttca attaaatgcg gcggcgcttg ggcgggcggc
    10201 agccgttatg cggaatgggg gtaacgtcct tgatcatatt tacctccaag ccggtggcct
    10261 gcaaagcacg gatagcggct tcacggccgg aaccagggcc tttcacataa acttctaccg
    10321 acttcatgcc atgctccatc gcggcgcggg cggccgtctc ggcagcagtc tgcgcggcaa
    10381 acggcgtgct cttgcgggaa ccgcggaagc ccaggccgcc tgcgctggcc caagacacgg
    10441 cattgccctg cacgtccgta atggtaacaa tggtgttatt gaatgtgctc tggatatgcg
    10501 cgcagccacg ttcaatattt ttgcgctcac ggcgtttgcc acgcacgggg gcgccttttt
    10561 tagcagcggc ttttgttgcc atctaactgt cctccttgtt ggattacttc ttcttgttgg
    10621 ctacagtgcg cttcggacct ttgcgggtac gggcgttcgt tttggaacgc tggccgcgca
    10681 cgggcaagcc cttgcggtga cgcgtgccgc ggtagcactg gatctcgaca aggcgtttga
    10741 tgtcgagcgc ggtgttacgg cgcaggtcgc cctcaacggt gaagttttta tcaatgtagt
    10801 cacggataag cgcttcttcg tccttggtca ggtccttcac tcgggtatcg ggattgatct
    10861 tcgtggcggc gaggatctcg tccgcagcac tgcggccaat gccgtagata taagtgaggc
    10921 caatttcgac tcgcttttcc ttgggcaagt caactccggc gatacgtgcc atggttttag
    10981 cacctcctga tttatttatc cccttggtgg atcatgccgc agaaggccgg cgggataaga
    11041 acgtccttgt gcggctggaa tgctgtttgt cgattaaccc tggcgctgtt tgtgcttcgg
    11101 gttctggcaa atcaccatga cgcggccttt gcgcttgatg actttgcact tttcgcaaat
    11161 cggtttcacg gaaggtttca ccttcatact tggtacctcc ttcaagctgc gccctgaatg
    11221 gttacttcga gcgccatgtg atgcggccct tcgtcaaatc gtaggggctc atctcaagtg
    11281 tgactttgtc tcccggcagg atgcggatga agttcatgcg cagcttgccg gaaatg
//
LOCUS       DAWBSB010000058        11313 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1346_length_11313_cov_2.975839, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000058 DAWBSB010000000
VERSION     DAWBSB010000058.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 11313)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11313)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11313
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1346_length_11313_cov_2.975839"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    249..258
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    549..558
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8571..8580
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2406 a         3296 c         3364 g         2217 t
ORIGIN      
        1 tggagcgatc tgcaccatgt cgaaataatc atcggcggag cgtccgtccc gaatgcgtgc
       61 agcattgacg tccatataaa aacgtacgaa ttcatagccg gtgagaaagt ccggcagaat
      121 gggcgtcgcg aacacataac ccacgtcgtc cggagtcagg gcgcggcgtg tgccgtcgtc
      181 cagcacaagc tccgctgtgc cgccgtcggg cggcacctcg tccgtcaggc agttgaacaa
      241 tgtggtttnn nnnnnnnntg cttgttctcc tttcgatcct cccggccacg gaaacgcgaa
      301 aaggcaccgc cggcaccctg tccggaaaag ggcgcggcaa tgccttgtct ccgggaaaag
      361 cgcagtgggc gcaggagcgg tgctgccgct gttctgcatg gtttcctcca atgaacgggt
      421 cagatagcgc ccttattata gcagacatcc acaaaatact tcaagaattg tcgcaggcag
      481 ctttgggcat tttcaggccg gaaaaactgg tttgtccggc gggaggagca gagactgccg
      541 ttatcgttnn nnnnnnnncc gcatgcaggc gattgcccca acagctcccg gctgagcgcc
      601 tcgatctgcg gcgcaagctg tgcgcgccgg cgttttgtca gatgcagaaa catcggataa
      661 gccagcgcca gcacggcgat gccggtgcag ccgacggcca cgcccagggc gaaaacacct
      721 gtccacacca tggtacagca catgcccagc ccgagcatca gcgtaccgca tacgccgagc
      781 gcgatgccgg caatggtacc gggctgctgg gcgcttttat ccaagcggcg cagaagctcc
      841 attttatcct cctggagcgg cgtgtatttt tggaggatgc gcctgacctc ctcctgctgc
      901 cgggcggagt aggtataggt gaaaatctcc ttgttttctt ccataaacag acactccttt
      961 agaaatgaat ggacggcggg gcagcggaag cagccgcgtt ctgccgcgcg gcccgccgga
     1021 gaaggatatt tccgcgtacg gccataaata cggccatgca gaatacaagc gagcacacac
     1081 aggctccggt gaaggcgttc attgtccggc gcatggccgc gtctccgccg aattgggcaa
     1141 gcatggcggt ctgcagggtc agtacgctca tcagcgcacc ggccaggccc agcatttttg
     1201 cggccgcatg cacggggtca tgcatgcggt gcgctttcac cgcgctggct gctgcgctgc
     1261 cgaagcagta aaacgtatat gcggctgagg catagatcag cgtgccggga taacggtatg
     1321 cgcggttctg ccagaccatt tgtgcggcta tggccgtcat ggccagattc agggcaaaca
     1381 tcagcacacc gcagcgccgg tagacacgca gcccgcgcat ctgccggaca ggagcggcag
     1441 acggcccggc gggcggagcg ttccgcacgc cgcgaagtac tgcaaaacgc atggtgctca
     1501 gaacggtgta atacactgcc gccgcaccga accagggtga gcggaaccgg atgcccgcgt
     1561 acagcttgca ggcggcgaag caaagattga cgcacagccc ggcgcgaagg gaaacaagcg
     1621 cgcgcagcgc ggcgtcggca cgccagcgcc gcaccggggg caggccgtag acagcggttt
     1681 ttgcacgccg ggctgcaggt actgcacggc aaatggccac gaccagtgca taggcggaaa
     1741 aggcataggc tgcgcgcgcg gcgggagcgt cctcccagcc gttggaaaag acggtccaca
     1801 gggaggccac ggcagcaaag gcagccagcg ccgcccaacc gccgccgggg aaaagcaggc
     1861 ggcgcagcaa aggctcaggg cgtttcattg gtttcggcgc gcagcaccac ggaaaacgtg
     1921 ctgcctttgc ccagcgtgct cttcaccgcg atctctccgc ccacgatatc caccacacgg
     1981 cgcaccagcg ccagccccag tccgttgccc tgcgttgcgt gggagctgtc gccctgataa
     2041 aatttttcaa aaatatgccg tcccgtttcg ggactcatgc cgcagccggt gtctgtgacg
     2101 cacacctcgg cctgcgtgcc ggtatgccga agggaaacat ggatgctgcc gcctgcatcc
     2161 gtgaatttga cggcgttgga gaacaggttg ttccacacca gcgtcagcag ctcgcggtcc
     2221 gtacggacgg ccacgccgtc cggaatgtcc gcctcaacag caagctcttt tgcctcccat
     2281 gcgtcctcaa agcccaacag gcattctcga agttgctcac ccagatcgta ggtctccgcc
     2341 tgcgggaaga tctgctgatt ttccagcttg ttcagtttga gaatattggc gatcaggtcc
     2401 gccaggcggc gggatgcatc tgtgacggcc ttggcatatt cgacgcgctg ctctttcggc
     2461 agggcagggt cctgcagcag ggaaccgtag ttctggatga cggccaacgg cgttttcagc
     2521 tcgtgtgaaa cattggcgat gaagtctgtg cgcagcgttt ccacgccgga aagctcccgg
     2581 gccatctggt tgaaatcctc aatgatggcg tcgaaggcgt cggcacgctc cgggccgtga
     2641 agcggctcga tgcgcacgct gaaatcgccc tggatgatgc gctgggtagc ggcgcggatg
     2701 cgccgcaccg ggcgctccac tgtcagcctg cgccggatgc cgtcgatgac ggtgaaaagc
     2761 gcgctcagaa gcaggatatt gagaaaagtg agcagcgcgt tttcccgcag gatatccggg
     2821 tcgagccgca tggaatcaag aaacagcagg aaagagcagg tgaccacgaa tgcgatcagt
     2881 gcgaaaaaaa tcaggaattt tgttgcggaa aacagatacc ggggaagccg cccggctccc
     2941 gagaagcgat cccggttcat ttcagcaccg ccttatagcc cagcccgtgc acggtgacga
     3001 tctcaaaatc cgggcaggcg gaaaatttgt cccgcagctt ggtgatatat acatccacag
     3061 tgcgcgggcc ggaggatgtt tccatttccc agaattcctc cataagctgt gcgcgggtga
     3121 aggtcttttt cgggtaggag agcagcttgt acagcaggtc gaattcccgt acggtgagcg
     3181 gaacctccgc gccgctcaga taggccgtgt gctcgtctgc gtccagcaaa aggctgcctg
     3241 ccgtaagcct tttgctgctg gcgatctttg ccctgcgcag cagcgcgccg atccgcagca
     3301 aaagctcgtc caggtccacg ggctttacca tgtaatcgtc ggctccggcg gaaaaaccgc
     3361 gctgtttagc cgtgagatca tcccgggccg tcataaacag gatgggcagc tccgtgttct
     3421 gctcccgcac ggtacgggca aattcaaagc cgtctgtccc aggcatcatg atgtcggaaa
     3481 tgataaggtc gaacacagtt tcatacaacg cgtcataggc ttcctccgca caaaggcagc
     3541 ccaccgcttc gtatccgttc cgccccagaa acgcgcagac cgcacggttc agctcccggt
     3601 cgtcctccac caccaatatc ttgaacaaaa catttccctc ctcaattttc tgctgctatt
     3661 ataaaatgcg attgttaaag ttttgtttgc gcgcgttccg tttgagaaaa tggcgaaaaa
     3721 agcgggagcg gcgttttggc cgcgcccgcc ccgtatggtc agcccgcatc ctgagcggcg
     3781 tcgaactggg cgcggtacag ctcgctgtaa tagcctcccc ggcgcatcag ctcgtcgtgg
     3841 gtgccgcatt ctgctaccgt gccctcacgc agcacggcga tgacgtccgc gtttcggatg
     3901 gtggacagcc ggtgtgcgat gacaaagcag gtgcgtcctt ccatcagccg aagcatggcg
     3961 gcctggatac ggcgttcggt gcgggtgtcc acattggagg tcgcttcgtc cagaatgagc
     4021 atcggcgcgt ccagcagcat ggcccgtgcg atggtgagca gctggcgctg gcccttggag
     4081 atgctgttgc cgccgtcccg cagcacggtg tcgtagccct ggggcagcgc ttctataaag
     4141 cggtcgatct tggcggcccg cgctgcagcg cgcacctgct ccagcgtcac accctccttg
     4201 ccgtaggcaa ggttttcgta tacggtgccc gaaaacagcc aggtgtcctg cagcaccatg
     4261 gaataggcgc ggcgcaggct ggcgcgggtg agggaaagga tctcctgtcc gtccacggtg
     4321 atggaaccgg aatctgcatc gtaaaagcgc atcagcaggt tgatgacagt ggttttgccc
     4381 gcgcccgtcg gccccactat ggcaaccacc tggccgggct gcgcggccag cgtgaagccg
     4441 tccagcaccg gcgcgccgcc gtagccgaag cgcacgccgc gcagtgccac tgcgccgcgc
     4501 acaccggaaa gctcccacgc gccgggagca tccggggcct cctctgcttc atccagcagc
     4561 tggaacacgc gctcggcggc agccagcgcg gactgcagct cgctgatgat gttggcaaat
     4621 tcgttgatgg gaccggaaaa tttgcgggaa tacaatacga aagaggacac actgcccagg
     4681 ctgatgccgc ccgccatgta cagcagcgcg ccgaacaggc tcaccagtgc cagagacaaa
     4741 ttattgatga agttgacgcc cggcccggta gaactggaaa aggcgtccgc ctcatagttg
     4801 gcgtcgcagg cgaggcggtt cttttcctca aaacgggaaa gaaacacctc ctcccggcca
     4861 taggccttga tggttttcag cccgcccgtc atttcctcgg caaagccgtt caaagcgccc
     4921 agaccggcag agcggcggcg gtacagcggc cgcacccgtt tggaacggtg tcgtgtaaac
     4981 atcacggaaa tgggaatggt cacaacgaaa ataaggatca gctgaggaga gatggagagc
     5041 atcattccaa aggaaccggc cacggtgatg acgctggtga gcatctgtac caggtcgttg
     5101 gaaagggagg cgttgatggt atccacgtcg taggagagca cgctcaatac gtcgccgatg
     5161 gcatgcgtat cgaaaaagcg cacaggcagg cgcatcaaat ggtcatagat gtccttgcgc
     5221 atgcggtaaa caacgttgcg gctcagccgc accatcagcg cggccagcac atagctcagc
     5281 gccgctgcgg caaaataaaa cacaagcatc tgttttgcat agtggaaaac cgtgttgaag
     5341 tccactcggc ccggcccggg gcggatggcg tcgatggcgt agcccgaaag cttcgggcct
     5401 gccaaagcaa gcagattgcc ggtaaggctg agcgccactg cggcggccag cagccatttg
     5461 taatggtaca gatagcgcca caggcgcctg agaatggctt ttgctcccgc gcggggcttc
     5521 tcgaaggcca tgctcggccc gccgcccatt cctttgctgc gtgccattta ttccacctcc
     5581 cccatttgga cctgcgcgat ctcgcggtag ctttcgcagg agcgcagcag ctcgctgtgc
     5641 gtgccgaggc ctgcggcgcg gccgtcctcc agaacaagga tacggtccgc gttctggatg
     5701 gagctgacgc gctgggccac gatgatcttt gtggtatccg caaagtcgcg cgccagcgcg
     5761 cggcgcagcg cagcgtccgt cttgtagtcc agcgcgctgg agctgtcgtc caacagcagg
     5821 atctccgggt cagccgcaag ggcgcgggcc agcagcacgc gctgcttctg cccgccgctc
     5881 aggttggcgc cttttgcggc caattgagcg gaaaaaccgc ccttacgctg tgcaatgaaa
     5941 tccgcctgcg ccgtgcgtgc cgccgcagca gtctgctccg gggaaattcc ccgcccgaag
     6001 tcgatgtttt caccgatttc atccgcgtag agaaagtcgt tctgaaacac cacgccgaac
     6061 ttgctgtgca gcacctcggg cggaatggaa cgaacgtcgc gtccgttcag ccgtaccgcg
     6121 ccgctgtccg ggtcgtaaaa acgcagcagc agccagagaa tggtggtttt tccgctgccc
     6181 gtcggcccaa tgatgcccag cgtctggccc cgtttcagag aaaagctgac gtctgaaagc
     6241 gtgtctttca ccttgtcata ggaaaaggag acatggtcaa agcggaggaa cgcgtcctcc
     6301 tgcaccggag gaatgttctg gggcgcaagg tccgcgggcg cggccagcac ctcggcaatg
     6361 cgccgtccgc tggccgcgcc cttggagtac ataacaaaca ggcgggagac catcatcagc
     6421 gcgttgagga tgatggtgaa gtaactcaaa aatgcgatga tggagccggg ctgtgcaagt
     6481 cctgcgttta cgcggtatgc gcccagcacg atgaccaccg tgagccctgt attgagcaga
     6541 aggttcatag cagggttcgt tacgttcatc aaaaagctgg cgcgctggtc gcagttcacc
     6601 gcacgggcgt tggcttcgtc aaagcgggcg cgctcatagt ctcccttgga cagcgcgtgg
     6661 atgacgcgga tgcccgccat gctctcctgc gcgcgccgta ccagggcgtc cagtgcaagc
     6721 tgtgtacggg tataaagccg tatgccccgt gtggaaacga accacaccac aagccccagc
     6781 agaggcagtg ttgcaatgag caccagtgtg agcaccggct ccagcgaaag cgtcagcgcg
     6841 ataccgccca ccagcaggat gggcgcacgc acacccaggc gctgcatcct gtccaccatc
     6901 tggtgtacgt tgtaggtatc gctcgtcagg cgggagatga gggagggcgt ggtgaatgca
     6961 tcctcctgtg cgcaggacag ctgcatcacc cgtgcaaaca ggtcgtgccg cagccgccgc
     7021 gtcacatccc gggaagtgga gcaagccagt cggttggcga ttacgttgcc gcccagcgcc
     7081 gccgctgcgc agagcaccat aagcccgccc cagaaaagga tgccgcccag cgctccggcg
     7141 ggaacagcct catccaaaat aacagagagc atccacggca aaagaagctc ggcaacggtg
     7201 ccgaaaaact tgatgagcag ccccacgctc ataatgccca gacgggggcg cagataatgc
     7261 agaactgttt tcattgaatg tcctccgcgc ggcgggccag gcgctccaga agtgactcca
     7321 accggtcgcg ttcttccgaa gtcaggtcat aaaacagctt ttcccgccag gaccggtata
     7381 cggcccgtac ggcggaaagt gtttcctcgg ccttctgcgt gggataaacc tccaccgcgc
     7441 ggcggtcgct ttcactgcgg cgccgtagga cgaagccgtt ttcctccagc atggccagct
     7501 ggcgcgttac gttgctgcgg ttcacgtgca ggcgcagagc cagctgatcc tgcgtaatgc
     7561 ccggcgcggc gcagatttcc ggaatataag gcgcctgcca gccgctcagc ccgcagtctg
     7621 caaggtgagc gtcccggtac agtgcggcac accgggagat ggtattgatg ttgcgcatca
     7681 aatgctgctt cataaaaacc tccttcgcag aaaacgcgcg gcaaaggcgc aaaatggtga
     7741 actgctgcgc gcgcattcgt tgcgcacgca acaatatggg ccgaacgacg ccccgccgtc
     7801 cgaatgggga aagcggggcg tatgcttata acaataacag tatacagagt gggcgaaaaa
     7861 aatgcaaccg ggaaaagcgg ttcactctgc cataagggaa gcgatcaatg catcacgcag
     7921 cgcgtccagt tgggccagag aatcgtcctt cagcgcggac ttcagcgtga ccacaggctc
     7981 caagatctcc atgctcttca tttcttccag aagggcgcgc atctgccggg cgcaggacgg
     8041 tgcccaggag ccgttttcca tcagggcaac ggtgcggttt tgcaggttga gcgccttcat
     8101 gtcgtgcagc agattcagca tggcggggta gaccccattg ttataggtgg gcgcggcaag
     8161 gatcaggtgg ctgcagcgga acacctcggc gatcatcgtg gaaacatgtg tgctggaaac
     8221 atcatagacg gcgatgtttt ttacaccggc ctccgccaag cgggaggtaa ggatatttac
     8281 ggcattttcc gtatcgccgt acatggaacc gtagaatacg gccacggagc gttcctccgg
     8341 tgtataggtg ctccaatgcc ggtatttgtc cagcagcctg tccaacccgg agcgccaaac
     8401 gggcccgtgc agcgggcaca ccatgcggat ctccacgccc gaaagcttgt tcagtgcggc
     8461 ctggacctgc gggccgtatt tgcccacgat attgccgtag tagcgccgcg tatccgccat
     8521 ccagccgcac tcaaagtcga tctcgtcgtc gaacaggtgg ccgttcagtg nnnnnnnnnn
     8581 ctgccgaagg cgtcggcgga aaaaaggata tgctcgtgct cttcataagt catcatcact
     8641 tcgggccagt ggaccatcgg tgtaaacaca aagcgcagcg tatgcgcacc aaggctgagc
     8701 gtatcgtttt ccaccacagt gatgatgcgg ccctccaggt cgaagtcata gaactggcgc
     8761 atcaaagtaa aggttttggc gtttcccacg actttcagct cgggaaagcg cagcagcagt
     8821 tcctcaatgt tggcgcaatg gtccggctcc atatggttga cgacaagata gtccagatgc
     8881 cgtccggcga gcgtatgcat gatgttttcc agaaactggc gcgagatgga ggcatccacc
     8941 gtgtcgataa gcgcggtttt ttcatccagg atcagatagg aattgtacgt gaccccgcgt
     9001 gggatgggga acagattttc aaaaagggaa aggcggcggt cattgccgcc gacccaggta
     9061 acggcgtcgc tgacagcgcg tgtgcagtac atgagagaca gctcctttct tgataaaaac
     9121 aggccctccg cacagcggca ggccgccccg cggagggagg aggacctgta tgcggagtgc
     9181 tctgcataca ggttcgatcc gggcggcaga ggttagcaaa agctaataga atcatagcat
     9241 attttgcccc gaattacaag aaaatgcctc tgcacatgca tgaaaaagcg aaccgtgctg
     9301 ccgcggcata ccggtcagtc cgacagaatg cgcccgtctg tggagataac tgctacacgc
     9361 cagggaatga atttgccgct ctgctgcaat gcgcgcttga cggcgttttc aatgccgccg
     9421 cagcagggaa cctccatgcg cgccacggtc acgctctgga tatcgttttc acgcaggatg
     9481 gcggtgagct tttccgcgta gtcgccctca tccagcttgg ggcatccgat gagcgttacg
     9541 cggccgcgga tgaaatcctc gtgaaacgca ccgtaggcgt acgcggtgca gtccgccgcg
     9601 atgagcagtc gtgcgccttg aaaataaggc gcgttcaccg gagcaagctt gatttggacc
     9661 ggccactggg acagcgcgct ggcggtacgg gggacggcgc tgtccgccgg ggcggcttca
     9721 cgccggatgc tgcgggcacc gctgccgggg catccgcagg gctgcggggc ggcgttctgc
     9781 gctttggcgg ccatgacggc cgcatggtca taggcgggag cttcgcgttc ttcaaagcgg
     9841 atcgcgcctg tggggcaggc gggcaggcag tcgcccaggc cgtcgcagta atcctcgcgc
     9901 agcagccgcg ccttgccatc tatcatgccg atggccccct catggcaggc ggcggcacac
     9961 aggccgcagc cgttgcattt ttcttcgtca atgtggatga ttcgtctttt catgtcattt
    10021 tcctcccggt tcatttgccg gccggggcgc gtggctgcac acgcacggcc tttgctgctg
    10081 taagcatacc gcgtccacaa gaaacagtat gttgttttta caacgaaatc cacatttttg
    10141 cagatacaga taaaaagcgc tccgtgcacc gtgcgcaaca aaaaaggccg atgctattcc
    10201 gcgcttgtgg tgaaaaaaca agagcggcgc ggttctcatt gcgtcgctct tgctggctct
    10261 acggtgcgcc aaggggatag gggagcgcgc ctgtgaccgt ttgctttggg aaaaatattc
    10321 ttctccgttt ttgcagctct gctgcaaata ggcttcggac cctgccaata tcggcataac
    10381 cgtgctcccg atcggttctc cgttttttaa gaggagtggc ctgtatcact ttttggcaaa
    10441 acggttcata aaaaaatctg tgaaagcggc gagcgcttcg gtctgtgaca catatacata
    10501 gagactctcg tcttccaacc agtcgcaggc attgataccg atgtagcttt ttctgtcagg
    10561 gtagataata aaggcatcgg tggggtactt gttccgatag gctttcagaa tttcagaacg
    10621 gcggtaatag gtaggtgcac cgcacccaga gagagccggt acggtgggaa gcgccacgat
    10681 cgtctgctct gcctcattcc aaccgacgat caaattccct acttttgttt tactgccgtg
    10741 gataaacccg taattgaacc gcgacacatc ctctgtgtag gcgaaaatca gttgatagtc
    10801 ggctccgttt tctaccgctt ggttgaacag cagacgcatt ttttcggcgt ttgcattgct
    10861 cttttccatg tcgatttcag gccccttaaa taatttgctg aaaaaaccca taaccattct
    10921 ccttgcttgt aaaacaattt tgttttatca ccacagcgtt tctgctgtca tgaataaagg
    10981 gggacatacg cttggtggac ttgccaagcc ctccataagc tcttgccgct ggaatccttg
    11041 catggctgtt ccaatcgcca gccgcacagg ccagaggttc tggaaagtgc gatacttcca
    11101 tgtgcgaatt ttcagtctca gagatatata aaactacagc agccaaaact gaaataaata
    11161 caaattttat aatttgtatt tattttgctc aaaaaactat ccggatgtgc ggcattaaag
    11221 catacaatcc ggataaatct atgcgatgca taccatcatc ggcagaagtt ttctttcttt
    11281 aaaggctcca ggaagtccac gatggccttg att
//
LOCUS       DAWBSB010000059        11022 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1384_length_11022_cov_3.800128, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000059 DAWBSB010000000
VERSION     DAWBSB010000059.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 11022)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11022)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11022
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1384_length_11022_cov_3.800128"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6883..6892
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2150 a         3279 c         3642 g         1941 t
ORIGIN      
        1 atccaattgt aaacgagccg atcaccttta agatcatggg tgcaaaagac cccggcgctc
       61 cggactggag cgagttggaa gtattccagc gcttgtccga gatcaccaac atcaactttg
      121 agtttgaagt gtccgagacc ggcacctggc ccgaacagaa aaacattgcg ctggtgggcg
      181 gcgagtacgg cgacatcatc ctgcgcgata agagtgcgac cgacgccaat atggccgtta
      241 ccgacgagga gacctacggc ccgcagggga tttttctgga cctgacggac ctcatcgacc
      301 agtacgcgcc caacatcaag gccatgatgg aaaaatatcc cgacgtcaaa gcggccatga
      361 cgagcatgga cggcaagatc tacggcctgc cgtatgtgtt ccacacggcc accgtacagg
      421 gccacactgc gtttttcagt gaagaatgga tgaaaaatgt gggcgttgat aaagtgcctg
      481 agaccaccga tgagctgtac gatatgctgg tcgcttttaa agaacaggac gcaaacggca
      541 acggcgaccc cagtgatgag atccccttca cctgcgtggg cctcaccacc accattcgcg
      601 acctgctcat tccggccttt acgggcctgc ccgacggcct gagctttaac ctggacgacg
      661 aaggcaaggt cgtgtatgcc ccggccaccg aggagtacaa ggaattcctg aaatatgcca
      721 acaagctgta cagcgaaggt ctgctggacc ccgagttctc cacccagacg gcacagcagt
      781 ggcaggctaa ggtaaaggct gaccagtgcg gcatttacag cggcagcccc accacgctgg
      841 acccctccac caccgcgcag cagctcagcc tgccgccgct caccagcgcc accaatgacg
      901 aaaaggtcgt caaacagccg ctttacatgt atccgggccg tgcgttcatc accgacaaat
      961 gctccgaccc tgtggcagcc atccgtctgc tggatatgtt ctatgccacc gaggaagacg
     1021 cagtggaagg cttcagcggc ctgacgacat ttgtgggctg ggaaggcgag cattgggaat
     1081 acaccgatga taccaagacg gcctattcct ggatcgaccc catcaccggc ttcagtgata
     1141 tcaacaaaac ggtgtctgtg aacatggagc tgcccggcct gctggaattc aatgcgatgc
     1201 ctgccggaag cccgctgatg gagatgaagt gcgagcaggt tgcagagatg caggctccgt
     1261 actataacat caagaatgca tatccccaga acgcgcgctt taccgccgag gaatccgagc
     1321 gcggcaatgt cattgaaaac gatctgtata cgcaggtgtt gatgatgaca acgaaattta
     1381 tcgtcggtga taccgacatt gaatccggct gggaggacta tcttgcttcc ctcgagaaga
     1441 tcggccttgc cgagctgacg gagatcaagg aagcggctct ggcccgctgg aacgaagcca
     1501 tccaataagt tttgtacgca cccggccggg gggcttccca agggggaaac agccccccgg
     1561 ccttttttaa aggccaatgg caaagaaaga gggaaaacca caatgatcca tacggtggag
     1621 accgcggcgc gcctgctgga gctgcccgca gctacactgc atgcgggcga cgtggcccag
     1681 gtgctgggcc gccttgtacc cggcgacggc agctggctgt accgctggga ccccaacagc
     1741 acagccgggc caaacggcgg cacggtgctt gcacccgcgg gcatgcccgc cgcgggccgc
     1801 tggcttttgt gtcacagcgg aacggtggat gcgcgctgct tcggcgtatt cggcccggac
     1861 gtcccggcag acgacgcgct ggacgcgctg atggcggacg ggagcgtaac gcgcatcgta
     1921 ttcggcacag atgtgaattt cacacgccgc cacatgttca cacgcgggca cgtcacgctg
     1981 gatttcaccg gccacacagt aacggcacag ggcgtggagc acgcaaagca caacgatccc
     2041 ttcggcgcgc tgtttcactt tacgggcgtg ccgggggatg acgtgcgtac ttttccgctg
     2101 gcgcagccca tgcgcgagct gtatgatgtg ttcgaggtgc ccggcgcggg cggcatcccg
     2161 ctgtattcct ggtggcaggt gagcgtcaac agcctcgcgg gccgtgagga aaaagagatc
     2221 gacaagctgc tgtgcgttac cgagcgcatc gacgaaagcc atgtgcgcgt taactataag
     2281 atgggatggc cgctggacgc cgggcgtgtg atgacctggc ggcgcgttga acccattgag
     2341 cgcgtctgcg tgcaggatat ggaattccgg ggcaacgagg gcggcgagga gacgggcgcg
     2401 cagccgttgg cctttgagta cgcggtgcgg tgcgatgtgc gggacgtgcg ggcgcggcat
     2461 acctactggc ctgtgctgct gcgccgccac aacaccgaat acgtcaccga acggtgcagt
     2521 ctggccaatc ccatagaggt ggtggtgggc ggcaccggat atctgacgca gcagatccac
     2581 tgtctgtacg gcaaggtgcg ggattgcacc acatccaacg cgcgccacct gaacgatttt
     2641 acgggcagcg catactgcat ggtggagaac tgtcacggcg acggcgattt tcacggggcc
     2701 tacgtcaccc acggccagtt cgagcacgac cttacctatg tgggcaattc cggcctgctc
     2761 agcttcgcca acagcggtcc cacctggggc agcagtgcaa agcgcatcac agtggtgcgg
     2821 cacacgggct tctggggcat cggcttcgca aaggtcagcg atcttaccct gcaggacgtc
     2881 gccgtctgcc gcacaggcgc ctacgacggt gtgaatacgg agggctacgg cccctgcggc
     2941 acgtttctgc tgaacgcgga cggcctgcag atgcgcggct gcacggctga aaagctggtt
     3001 ttgacccagc gcagccgtcg cgcaaagcgg cccgcgatcg tggagggctg ctggttccgg
     3061 gacgggatcg aagtggtgcg gacgggcgag ggagccgtcg cgcccggcac gccgctgatc
     3121 ctgcgagaca atctgacggg ccccggcggc ccgcagcaag cggagggaac agaatgaacg
     3181 acatccatat tctggttacg atgccgcagc atgcggtatt caggaccttt tttgacgccg
     3241 acgccatggc tgcgctggag cacatcggca cggtacactg gaacacgctg ggccgccagt
     3301 acacccagga cgagttgggc gcggccgtac gcggcatgga catctgcgtc acaggctggg
     3361 gaaccccggt ctttgatgaa gcggtgctgg cgcaggcgga ccgcctgcgg ctcattgccc
     3421 acacgggagg ctccgtcaag ccctatgtga cggacgcggt gtatggccgg ggcatccgtg
     3481 tggtgagcgg caacgaggtg tttgcggagt ccgtggccga gagcgtggtg gcctatgcgc
     3541 tggccagcct gcggcagatc ccctatcact ctgtgaacct ggccggcggc atatggccgg
     3601 agagctttgc gaaccgcggc ctgctggacc gcagcgtggg cattgtgggc tacggcatga
     3661 tcgcccgcta tgtggtgggc atgctggcgc cgttccactg tcccatcaag gtgttttccc
     3721 ggcacatcgg ccaggacgag ctggaccgct gccacatgga aaaagccgag ctggaggaac
     3781 tgttcgccac ctgcgacatc atttccatcc acagcggcat gacgccggaa aaccaccatc
     3841 ttgtcacgcg ggagctgctg cagagcatga agcccggcgc gctgcttgtc aatacggcgc
     3901 ggggcgcagt catcgacgag ccggcgctgt gcgaggtgct ggccgcacgg ccggaccttt
     3961 ccgccgcgct ggacgtgtac gagaccgagc cgctgcccgc gggccatccg ctggaaaagt
     4021 tgccaaatgt gctgctgatg ccccacatgg gaggccccac ggtggaccgc cgtctggccg
     4081 tcacccgcag cgtgacgcag gatatccggc gctttctggc agggcagccc atgagctgcg
     4141 agatcagccg cagttatgcg gcgaaaatgt cggcgtatta aagcgcgccg cacgggaaag
     4201 gcgggccgcc gtgcgcgccc gcagcaaggg aggaacagga aatgaaacag aaactgaagc
     4261 tggcgctggt gggcgcggga gagcgcggcc aatactgcta tgcgccttac gcaaaaatgc
     4321 acggctacga aattgagttc gtggcggtgg cggacccgca taagggccgc cgggaaaaat
     4381 tcgtccagga ttaccatgtg cccgccgaag gcgtgttcga gacggcggag cagtttttcg
     4441 cccggccgcg catggcggac gcggtgctca tctgcaccca ggacagccag cactatgcgt
     4501 atgcctgcca ggccatcgac ctggggtaca gcatcctgct ggaaaagccc atctcgccca
     4561 gtgtgcgcga atgcctggac ctgcagcgcc gcgccgagga aaagggcacg cagatcatgg
     4621 tctgccatgt gatgcgctat accaaattct accgcaagct caaggagctg atcgactccg
     4681 gcgtcatcgg cgacgtggtg cacgtcaccc ataccgagaa cgtggcctac tggcactacg
     4741 cgcacagcta tgtacgcggc aactggcata agacggcgga ttccagcccc atgatcctgg
     4801 caaaatgctg ccacgatatg gatatccttt cctggctgct gggcagccgg tgcagaacgg
     4861 tctccagctt tggcgacctg cgctatttcc gggaggagaa tgcccccgcg ggcagccccg
     4921 cccgctgcac cgacggctgc ccgcacagcg gctcctgccc atactacgcg cccgcgctgt
     4981 acctggacga caatacgccc tggcccaccg cgctgacggc cctggggccg gaccagagct
     5041 atgaggcgcg caaaaaggcg ctggaggaag gcccctacgg taagtgcgtg ttccacaacg
     5101 acaacgatgt ggtggatcat caggtggcca gcctcctgtt tgaaaacggc accaccgtgg
     5161 cgttcacgat gtgcgcgttt tccgacgcat gcgaccgcac cgtcaaattt atgggcacca
     5221 gaggcgagat ccgcgcttcc atggacaaca acgtcattga ggtgacgcag ttcggcgcgg
     5281 gcgtgcgaac gggcaccact gcggtataca ccgtcaagcc cggcagcacg ggccactccg
     5341 gcggcgacga gggtattatg gaagaatttg tctccatcct gaagggcgaa cgcgagaata
     5401 cgaacaccat cgcccagtcc gtccacagcc atgtgatggc ctttgcggcg gaggaatccc
     5461 gcctgacagg ccgcaccgtg gatgtggcgg attttgaaaa aagcgtgatg gcctgataac
     5521 caggcttttt cctgcggcgc agcaaaaaag aaacggcgcg gacgggcttt tcagccaaag
     5581 gtgtggtata ctggctgaag gggcgtccgc gccccttttg taaaaggaga acagggaagg
     5641 ggtcgtttgg atggttcatc tggaacaggc gcagccggag cagctgcagg agcttttggc
     5701 gtttgcggag gaggcgttcc gcacgccgga ggggaaggtg gatttcccca cgctgctgcc
     5761 caagctgtac gggccgggcg ccgacagcgc gccgctgcat acggtgctgt atgaggacgg
     5821 cgcgcccgca gggctgtact gcctgaagat acaggatttc aacatagctg gaacagccct
     5881 gcgcgtggga ggcatcggca cggtgtgcgt ggacggacgc agccggggca aagggcatct
     5941 ggccctgctg atgcaggacg cgcaggagcg tatgcaggcg gcgggctgtg acattgccat
     6001 gctgggcggc cagcgccagc ggtacgagcg cttcgggtat gtacccgcgg gcgcgcactg
     6061 ggagtttacg ctcacaccgc gcaacgtgcg ggatatccgg acgggcggcg tggcgctggc
     6121 gcctctggcg gaatacccca gctggctgga gagcgcccgg gcactgcatg aaaagcagcc
     6181 cgtaacgtgc gcccggggcg gggacgcctc gttcctcacc gtgctgcaaa gctggcgcgc
     6241 cgagccatat gcggttttgc aggacggcat gttcgcgggc tattgcgcgc tggcggaggg
     6301 aaaaccgccg cggctgcagg agctggtgct gccggatgcg gcgctgttgc cggcctttgc
     6361 ggcggccctg gcacagcata cgggagctgc cggtgtacgt gtacagcttt acccgtggca
     6421 gcgggcggag cttgcctatt ttgcctctgt ggcggaacag ggcgtttgtg tgccaaacca
     6481 cagctaccgc gttttccatt gggcggctgc ggcccgcgcc gcaatggcgc tgcagcgcgc
     6541 ttatgacgat acggcgattt tagatgaatt tatacttgaa gtagaaaatg aggggcgtct
     6601 gcgcttcggg ccgggggcag agggggtgcg ctccgcggag ccgcaggatg cggcgccgga
     6661 ccttgtgctg ccgccgctgg acgccgcgcg tctgctgttc gggccggaca gcccgctggt
     6721 gcccggcgcg caggccgttc ccgccgggct gctgccgctg ccgttggcct tcccctgggc
     6781 ggacggcatt tgaatggggc ggaagaaagc cgactttgcc tgcaaaagcg gccgcgggcc
     6841 gttttccggc cgggcccttg agaaaaaaca gcccgctttc ctnnnnnnnn nnctttcctc
     6901 cggatgtaat attccggaag aaggcgggct gtttgctttt agggttctgc ctgacaaagc
     6961 ggcggctgcg gtgaaagaaa gcgcttttgc gcttcatttg tgctgcgcac ggtaatcgcc
     7021 gggcgtggtg ccctcgtact tgcggaacag gcggatgatg ccgatctcgc ccgcgtagcc
     7081 cagccgtgcg gcaacctccc gcaccggcag gtcggtgccg gtcagcagcg ctttggcctg
     7141 ggccagcttg cgtctggcga tgtaatcctt gacattttcg tcccggtact ttttgaatac
     7201 gttggagatg tactgcggcg tcacgccgaa cgcttccgaa agaacggtaa ggtcggggaa
     7261 ctccgattcc gcgcagttgt cgatgtacgc cgcgatgcgc tccaccagct tttccgtttt
     7321 gctttgggcg cggttttcat gggcagcggc aagccgggcg atatgctcgg acaagcggcg
     7381 gcgggcgtag tcgatgctgg agctgttcac cacctgctcc acgagctgtt cgtcaaagtc
     7441 cgcgccgccg ccctgcgcca gcgcgtcact gttggcaagg cgctgcagcg tggcggcaag
     7501 ctggtacagc aggctgcgcg cggcagccgc gctcacacgt tttgtttcaa agtttgcggt
     7561 aaagatgcgg cccagcacct cctgcgcctt ggccgcgtct ccggcgcgca catattgcag
     7621 aagctgctgt tcggcctcca gcggataata atagtccgaa tgggtaacgt ccagagccgt
     7681 gaacagcacg ggctccgtgc cgcctccggc ctgcaggcgg ctgtactcca gcgcctttcg
     7741 ggcctcgtcg tagcaaaggg gaagctgctc cagcccctgc tccatggccg aaacgcctac
     7801 aaagatatca agctcaaact gctgtgcgca gaagcgcgcc agggcgtccc cctggcggga
     7861 tgccgccgcc aatgcggcgg catcattctc tcctgaaggc ggcgaaagca ggaatacgcc
     7921 ctgtgcgcgg cccaggtcca gatactggca ggccgcgttt gcgccgaaca gctcgcaccc
     7981 cacgttctgc agcacgagcc gggccacgga aaggttattt tccagcggga tattggaatc
     8041 caggaaaaag gcgctgtcca ggtccgcctc ggccagtacg gcgatatatg catcgccgcc
     8101 gaaacgcacg ccaagggctt ccagctggct gcgcgcgccg tcccagtcca gcgtctgccc
     8161 cagcaggagc tggagcagca ggtcccgcac tacggcgggg cgctgggcct cgatgatgcg
     8221 ctgcagctcg gtgcctgagc gcatctgatt gagcagcgtg gacttgatga cggcgaattc
     8281 gtcttcgccc gcgccgccag gcgcgctttg taaaatcagg tcgttaatgt ccttcaccgg
     8341 gcggtagctg cgccgggcga tgcgccgcac cagaaacagc cccagcagca ggtatacggc
     8401 aaatacacca cccaggaagc cgatggtgga aaggttgtcg gtaaaataaa gattggcggg
     8461 cgacgtgata aagtaggtcc agcccgtttc ctcggagaca tagcgtgtgt aggagatatg
     8521 gtccgcggtc accagggcct ccgcgctgtt cagcactccg gcgggcaggc cgggcgcgtc
     8581 cggcgtttgg tacacggccg cgcctgtttc atccagcaca tagacgctgc cgcccgtagc
     8641 ggactgaaga gacagcgcat gcgcaaagaa tttgtcgctg tccagcagga gcatcacctg
     8701 tcccggcgga ggcgcgctgc tgctgatggg gatggactgg acgtagggaa tgacgcggat
     8761 caccgtgtcg gaatcgtact gacgcagccc gcccgcggga agataatcct gaaaatggta
     8821 ttccgccagc gtgccttcgc gcagcgcctg ggctgtgaga tcggtaaatt cgtagatgat
     8881 atcgaaaaac ttgtccgcgc tcatccgcac cgtggaggtg acgacctcgt cggtgctgcg
     8941 cacataaaga aaataatcca gcaggaaatc ccgcgaaacg gccatcccgg ccatctggtc
     9001 catcgcgtcg tgcacggagg aaacagacgg atgcttcaca aggaactgca gccgggagtt
     9061 gaacaccagg ctgttgccca gctcctccac ataacgcagg ttttcctcta cgtcgttttt
     9121 cagtgaggtg agcagggcct gcttattgag ggcctcctcc cggcgcagct gctgggcgac
     9181 ctccgctccg aaaaacagac aactgctcag agagacgagc agaataaggg tatacgaaag
     9241 caggaagaac gagtatacgc ggctcttttc ctttttggcc ctgtgcggcg cggctttttc
     9301 tgtcggcatc ccggcgttcc tcctcccggc gtcaatgcgg agttcccgcc gggaaaagcg
     9361 gcgcggcgcg cggctcccgg cgggaacgct ctgcttataa tggtaaggga aaaagagcgg
     9421 tttgtcaaat gaaaccggcg gctgtataat agttgtaaga agattttgta caaacaggcg
     9481 gaacggggga gaagcatgac ggaactggca gtgctggact ttgagacaac gggccttgtg
     9541 ccggggcagg acgaggtgct gcaggtggcg gtggtggatg agaccggagc ggtgctgatg
     9601 aacgaatact gccgcccaca gcgtcacacg gcgtgggccg acgcgcagaa ggtgaacggc
     9661 atcgcaccgc aggacgtgtg cggcaaacct cccttcgagg cgcttgtgcc gcgcctgcag
     9721 gagatcttat ccggcgcgcg gcgggtggtg gcttataacg acgggtttga aaaagccttc
     9781 ctggatgcct acggcattcc gtcccgcggg ctgcgctggg gggaggaccc aatggccgct
     9841 tttgcacagc agatgggcgg gcagaagcga agcctttccg ctgtcgcgtc gtttttcggc
     9901 tatgaattcg aggcgcacga cgcgctggcc gacgtgcggg ccacgctgta tctttacgag
     9961 cggctggcgg acggcgggct gctgcgctgg gcaacgcgca atgcgtcgcc cgcgtggggg
    10021 cagtcgcccc ggggcccggt agcataccag acgctgccgc ccgaggacat gctgcgcctg
    10081 ctcaaatgcc tgggcctgtg cccgctgacg gcggcgcagg cccggcagct gtggtataag
    10141 ggcatctata ccgcgtcgcc ccgcccctgc gagattgtgg ggtttcagaa ccgttccaaa
    10201 gaggacggct ggctggtggt cgccgtggag gcggacggca tgacgtatct tgtgtgcgac
    10261 gattatttgc gggagatgca ggctccggcc tttggcggag atggcgcggc ggcgtccgtc
    10321 cccgggcagg ccgtggggac gggacggaca gggcgcgcgt cccgggccgg tatagccgcg
    10381 cgtggaaaag cggaaggcgc ggcaaacagc cgctacgcgg cctttgcccg caagagtgca
    10441 gccctgaaga acgtggaggc aaacccgaat gcagacccct ccaacccgtt ttacggcaaa
    10501 gccgtcgtat ttaccggcga catggagatg ccccgggccg aagcagccca ggcggttgcg
    10561 gagctgggcg cccatgtgaa gagcgccgtt tcccgcaaga cggattatct ggtcgcgggc
    10621 gcgcaggacc cggcgctggt gggcgcgggc gggcgcagcg gcaaggagga aaaggccgcc
    10681 gctctgaacg aaagcggcca gggaaaaatc gagatcctgg acgaagccgc ctttcgcgcc
    10741 ctgctggcgc aggcgcgcgg gcagtgagct ttgtccgccg gctcctgtct ggaaagaatg
    10801 tacggctgaa cgccctgtct cgagtgcggc ggcagcttgg acatccaatg cgctcaatct
    10861 ccataaaaat ggccatcggc tgtttttggg tcatgcggcc cgtccccggc cggcggccat
    10921 tgagataagt tttttatagc gggacaataa agaatcccgg cccgggaggc gtttgcctcc
    10981 cgggccgggt gcgcgatacg gctgcgcggg atgagcgccg cc
//
LOCUS       DAWBSB010000060        10995 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1387_length_10995_cov_3.413711, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000060 DAWBSB010000000
VERSION     DAWBSB010000060.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 10995)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10995)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10995
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1387_length_10995_cov_3.413711"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4891..4900
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2055 a         3556 c         3203 g         2171 t
ORIGIN      
        1 gtgccaagac gtgtagaacg aagcggccgc cgaaggccgc catgtcgcag cgaccgcggc
       61 cgaaacccct gttcgcaggc agattggcga aaaagccgcg ctttcccgcc aatccggatg
      121 cacttgccct gccgggaagc tgcttttccg cattctgcct gttcctgccc ggcagaaaac
      181 cggtctgtgg aactgtgccg tccatgtgtc ctctcttttt tcgatttctg ttttgcaacc
      241 ctgtttccaa tcatttataa taaaggagga ttcctaacat gtccgatccg gaatacatcg
      301 ggcgcatcgc ccgcagcatc gcgccgtgcg ggctggtctg cgccctgtgc ggcaagccgt
      361 cctgcaaggg ctgccgggag agtacggatg ccgagctctc ctgtttgtgc cgccagcgta
      421 catgctgccg ggagaaaggc ttctccggat gctgggaatg cccgcttttt ccctgtgggg
      481 agggcatgtt ttcctctgcg cggagcagca gacgccttac cgcctttgtg cgctgcatgc
      541 gggaggacgg cgcagacgcg cttgcgtcgt atctggccgc caacgaagcg cagggcctgc
      601 tgtaccatcg cgacatcgta cattttaccg gcgattatga cataccgaca aacgaagcgg
      661 agattttgcg tctgctgcga aagggcccca aaccatgatt ccgggcgcat ggcgcgcctg
      721 atttttgcaa agaggtgttt tgaatgaaag tactgctgtt gaacggcagc ccccatgcga
      781 agggctgtac ctacactgcg ctggctgagg ctgccggcgc gctggaggct gccggtgtgg
      841 aaacggaatt tgtccatatc ggtacaaagg cgatccgcgg ctgcatcggc tgcggcaaat
      901 gccgggaggc aaagcgctgc gtttttgacg acgatcctgt aaatgaggcc atcgaaaaag
      961 cccgggcctg cgacggcttc attttcggtt cccctgtgta ctatgcctcg cccaacggcg
     1021 ctgcgctggc gtttctggac cggttgttct acgcgggcgg cagcgcgctg cggtacaagc
     1081 ccggcgcggc ggtcgtctcg gcccggcgcg gcggcacaac cgccgcgctg gacgcgctga
     1141 acaaatattt taccatcaac tgtatgccgg tggtatcttc aaaatactgg aacatggtgc
     1201 atggcaacac gcccgatgag gtgcggcatg acctggaagg catgcaggtg atgcgcacac
     1261 tgggacgcaa tatggcctgg ctgctcaaat gcattgagat cggccgcgcg gccggcatca
     1321 ccgcgccgga gccggaaccg cccgtgcgca caaatttcat ccgctgagga cgctcttttg
     1381 ttcaacaacc gccgcccggc ggacattccg gaggcaaaaa atgacccctg aaacaaacgc
     1441 gctcattccc gtgggcattg ccgcgctgtg ctttttactc gcttttctgt atggacggca
     1501 ccggcgggca ctggcgctgc gccgccgtgt ggcggaaagc ttcggtcaga cgccggcaga
     1561 gccggaacgg ccccgcgcca tgacacggga attctgggaa ctcctgcgcg caggcgagcc
     1621 tgccggccag tgcatcgacg acgcgacctg gaacgacctg gacatggacg acgtgttcgc
     1681 gcgcattgat atctgccaaa gcgccgtggg gcgtgcctgc ctttatgcag cgctgcaccg
     1741 cctgagcacc gggccggagc ttgcccggcg cgcccgcctc tgcggccttt tcgcggcgcg
     1801 gcccgcgctt cggctgaatg tccagctctg ccttgcacgc ctgggccgca agcgcgggca
     1861 ggaattcgcc ggtttcctgt ttgacccatc tcccatgcgt ctgccgctgg ccgggttgta
     1921 ccgcatttgt atgtttcttc cctttctggc attgccctca cttttcttcg ggccgtgggc
     1981 gttgtgcttt cttgccgcct gcgtgttgaa cacggcgctg tattatcacg ccaaagataa
     2041 aatacgcgcg catctggaca cattgggtta tttttcaatg ctgctttctg cggcaaagcg
     2101 cctttcccgg ctcgtgcaag ccgacgcgcc ccagctttcc gatgcgctga acgatgccgc
     2161 ccggccgctg aaggcggttt ccggcggcgt gggggcgctg gctcgcccct ccacgggcga
     2221 gctgaacgac atgctgttcg agctgatggg ggcactgacg ctggcgcccc tgctgtatta
     2281 cgaccgcgcc gtccgtgtga tgcagcgcga acacgctgcg ctgctgcgcc tgtgcatgct
     2341 gctgggcgaa acggagcttg ccatagccgc gctttcattc cgtgagagtc ttcccctccg
     2401 gtgtacgccg gaatttactt ccgaaatggc acttgtctgc gacgaactgt accatccgct
     2461 cctggaaacg cccgtggcga acagcgcggt attccggcgc cacacattgt tcactggttc
     2521 caatgcgtcc ggcaaatcca cattcatcaa agcactggcc gtcaacgcca ttctggcgca
     2581 gacgctgggc gtatgcgccg cccgtgcatt cacgctgccc cgggcgctgg cggtatcctc
     2641 catggcggtt tccgacaacc tctgcgccgg agacagctat tttgtggcgg agctgaaatc
     2701 tctgcgccgc ctgctggcct gcgcgcaaac cggaacgccc tgcctgtgct ttattgacga
     2761 gattctgaag gggaccaaca ccgtggagcg catcgcagcc tcggcggcgg tgctgcgcca
     2821 tctggccggg tgccgctgtc tgtgcttcgt cgccacccat gaccgggagc tgacggaaat
     2881 gcttgcgggc ctgtatgaaa accgccactt ctccgaacag gtggggccgg agggcgtcac
     2941 ctttgactat ctgctcaggg agggtcccgc gcacacacgc aacgccatcg cgctgctggc
     3001 gcagttgggt ttccctgcaa aggtgacgca ggcggcggag gatgcggccc gcgccttcga
     3061 ggcggaggga cggtggcccg cggaacggag cggcagaaag gataattgtg gcacctgctt
     3121 tccggaaacg ccctgcagct gggacaaagc agcgaaaaaa tgatgtacag gctgtctttc
     3181 tcgccctgaa agcacccggt acgccatggc ctatgtgaat accggcggcc ttcaccgcgg
     3241 cttatgcgct ttcccgcatc gacctcgtgc ccgattttat ccctatgccg agatacccgg
     3301 acgacctttt gcttttgccg ctgtcggcgg catgcaccgt aaggcaaatc ccgccgcaga
     3361 tttttaccgc atgcagtcca cgggccgatg gtctggtctg tggaatgaag gcagacccaa
     3421 acgctggtac tgcgccattc ctaccgctcc ggcatgggcc ctggggctgc tgcttttgaa
     3481 agccgtgctt tgagaaagcc cgcgcaggac cgccccccgg acggaaagga gaaattcctg
     3541 aatgcatatg cctgatgcca ttcccgaaaa tatgttcgcg ccgtgcggca tgaactgcat
     3601 ggtatgctat aagcactgta ccgtttgcaa aagcgtgcgt ccctgtgcag gatgcctgaa
     3661 cggggacgaa ggcaagccgc gtcactgccg tgcctgtgcc atcagggact gctgccgtga
     3721 aaagggcctg cgctattgcc ttgaatgcac cgcgttcccc tgccggcgcc tgaaccgtct
     3781 ggaaaaaagc tatcgtacgc gctacgacgc cagtcttttg tccaacagcc gcgccgttct
     3841 cacacgcggg cttgccgtct ttctggagaa tgaacggcaa aaatatacct gttccgtgtg
     3901 cggcggcatt gtttcgctgc atgacggtgt gtgcggaacc tgcggcgcat ccacggccgc
     3961 gctggaatag aagcatgcgc tgcgcggtgc cggtcgcgga aaaaggaacc tttccgctgc
     4021 gcgatttttg tgctatactc ttttggtggc tgcaagcgta tcggggcaca aaggtgccgg
     4081 gacgcccggc ggacgcggca agatccgcgc agccgccgca ggaatgaaat agaaacggag
     4141 aaatgaaaca tgatcgagat caccatggaa aacggcaaga ccatccgcat tgaactggac
     4201 gccgaggccg cgcccatcac ggtggaaaat ttcacaaagc tggtgaaaca gggtttttat
     4261 gacggcctga cgttccaccg catcattccc ggctttatga tccagggcgg ctgtcccgag
     4321 ggcaccggca tgggcggccc gggctatggc atcaaaggcg aatttgccgc aaacggcgtt
     4381 gaaaacccca tccgccatac ccgcggcgtc atcagcatgg cccgcagcca gatgcccaac
     4441 tccgcgggca gccaattctt catcatgcat gccgacgcgc cgcatctgga cggcagttac
     4501 gccgctttcg gccatgtggt ggaaggcatg gacgttgtcg acgagatcgc gtccgtcgcc
     4561 acggatttcc gcgacaagcc caaaacgccc gttgtgatga agtgtgtgaa ggtcgtggac
     4621 tgagatgcgc ctgtttaaaa aactgttctc gtttttctgg gacaggagcc tgctgatctt
     4681 tcttatcatc ggcgggctga acacactcgt cagcatggtc ggcagccagc ttttgtacgg
     4741 cgcgctgggc tattggggcg cgaccgcgct gatgttcacc gtatgcagcg tgttcagctt
     4801 tatcttcaac cggaaataca gttttgaaag caaggcccct ctgctgcaaa gcgccgtgcg
     4861 cttttctgtg gtcatcgcgg tgtgctacct nnnnnnnnnn cagcttcggc ctttcggata
     4921 ttctggtgcc gatgttcatg gggcgcttct ttcccgctgt ttccacagac tggcaggcgc
     4981 gcatcgccat gctggtagcg caggtgatct ttaccgcctg caactatctg gggcagcgcc
     5041 tgtgggcttt taaagaataa gcccgttccg catcccattt ttgcaaaaaa gcctttgccc
     5101 cggacattgc gtcccggggc aaaggctttt tccgtatctg caatcggcgg tgccgggccg
     5161 gagacgggat ggcctgcggc cttttcgcgc cgcgctccgt tccatcactc ccgccgcggc
     5221 gcaccggcag cgcttaaggc tccggcccgc gtctcctcct cttcatgaaa cgcagagtgc
     5281 tcaccgaagg agggcacgaa caggaatttc cggatggcaa agatcagcgc gatggtgacg
     5341 atgccgatga gattttccag cggcgaagta tgctccacca ccatctgccg ggcgatggcg
     5401 aacagaagca cctcaatggc ggagccgggc gtatgctttg ccagcatctt gataaactcc
     5461 acgccgatga tcaggttgaa cgcgtggccc aaaaacactg taaagccctc gtgggctttc
     5521 ggatcgcccg caaggctgaa cacctcgcgc accacctgta tacccgcgat aacaatggcg
     5581 atgagcacga tgatggaaat ggcgatttca ataacgcttg caaaaaacgc gaccttgcgc
     5641 cgtaaatatt ccttgacacg cacggcaaat cctccttttc tgctgctccg tcccggccgc
     5701 gctcagccag ccaaaggcct gtggatacac gctttgcggt aaaccgacca aacgaacagg
     5761 caaaccgcgg cgttcaccgc cagaatgatt ccttcaatgc accacatgct ccgcagcagc
     5821 cccaactggg acagcaccgc gggaatattc gccgcggcat gcagcagcac cgccgcgaaa
     5881 tagtaccaga tgggaacgcg ccgggtgacc gccatccaca ccaggatgga cagcgccata
     5941 tgcagcgcca ttgccgcgat gcgttctaca ccggcagccc agaacgcgcc cgcccccgcg
     6001 gccccaagcg cgtccgttac ctgcggcgcg gcgggcgcgc ccagcatcac cgcagcgttc
     6061 acggcggcgt tcacccctgc cagcagcgca gcctccacgc cgccgtggcc aatgccgtag
     6121 cctacgccca acgccagatc cctgtctttt ttgcacagca gcgacagacc catcaggcgg
     6181 cctgtttcct caaacaggcc cgcggcaagg cagccgtaag ccgtataggc cgccggatac
     6241 agcgtaatag ccggaaacag ggagaacacc gccgcgtgga gcagctgctc cagcaccaac
     6301 gcatagccga tgaaacaacc ggcgcccacc agcgcggcca gcaagccgcg gcctgtttta
     6361 cgcttgagga cggcaagcgc cgcaacaggc agtgcgatgg aaaccagcac cgtaaaaatg
     6421 catcctgcaa tggatgcggc tgagaagacg ggggcagcag acacagagca tcctcctttt
     6481 ccggacgttg acaccgctta ttgaaacaga gaacctgaca gccgataccg gagcgtccac
     6541 ccctttttgg cggcattcgc cgcgtggctt gcactttgcc gcgcaaagat gcatggcctt
     6601 gccaccgcca gctttttccc cggatttttc ttttttatct ctttgccttt ttcgacagtc
     6661 tgcgtccgtc ccttttcaag aagcggacgt ttctccggtt tgttacggtt tttttgcaaa
     6721 gggaacggat gcgatgatgg cgcaccatac gagctgatac gccagcaccg tgcccagaga
     6781 aaggcccgta aggccctcgt aaaagcccaa cgccacactg agcaccgcac ccagcgccac
     6841 agcggccgtc tgcacaacgc ccgcaaggcg ttcgcctgcg gccgcgcgcg cggccgcgcg
     6901 catgccgccc aggaaggacg cgcatgcacc tgtgtgcatc atcacgccct cgctctcagg
     6961 acggtaggca agctcggtct ccagggcgtc ctgctcatgc tgagacagca ccttgaccgt
     7021 tccctccgga atgccgtaag tcgcggccac cagcggcgcg gtaatgttga aatcgtctgc
     7081 ctgcaccagc acgctgatac cgctctgcgc cagactgtcc aatgtttctg cggcgcggcg
     7141 gtcgggccgg tagctgacga ggaacatgcc gaacagcttg cccgccaccg caagatagat
     7201 gggagagcgc tggccgttct tcgtatattt tttctcgtag tccaaagagg gtacttcaat
     7261 gtcatggcgc ttcatcatct cgcggctgcc cagcagaaca cggcggtgct cgatccaccc
     7321 ggtaaagccg taaccgattt ccaccgaggc gttttccaca ccggccagca gcttctcgtt
     7381 gttgtccagc atgcccatga acacgccgcg cagcgtctcg cagctgggcg acagcaggct
     7441 ggctgcataa aggatggcaa tgtcgatgcg ctctttttca aaggttttga tgccgtgcag
     7501 gcgcacgctg cccgcgggga acagctcccg cgcgctcagc agcaccgtgt tggcgctccc
     7561 cagtgtttcc actgcgctcg gccccggaac cacggcgccg cagcgggacg tggcctgctg
     7621 catcaagctg gtgggcagcg cgtacacaag cgtcgccgcc agaggcgcgc tcaggctcaa
     7681 ggccgcagcc aggccggaga gcgcagatat cacgccgccg ccctttacgc ctgcgacaac
     7741 agcgcacacc agcgccgcgc cgcccaggcc ccagcacagc ttctgcacca tggcgtccgc
     7801 cgcgcgggga gaaaagctct ggcgcaaaaa gcccttgacc agcgctgtag gacggttcag
     7861 cagcagacgc ggttccggct cgcccaaacc gtcgcacagc cgctttgcca gctgttcctt
     7921 ttccagcaga aacgcggccg catgctcctc gccggagctt gccagcgcaa aattcgcgca
     7981 cacagcgcgg atctgcagcc atttgcccag cgcgttgccg cacagcagca gcgccgccac
     8041 gggcgcaaac agcgtcacgg cctccggatc aaaatttttc gcatcaaaca gcagcgccgc
     8101 attctgcagc gccgctccca ccacggccag cgcagtgaag ctgtcggacg taggctggag
     8161 cgcaaggccc cgcaggcctg cccccagcgt ggtgatggag ctgaaagccg ctacggccaa
     8221 cagaacgaaa tttgtgacaa gataagtaag cggagccgtg tgcgggtcca gcacgccaat
     8281 gggcggcagc cagcccgtgc gggccgagag gcccaaatac aacaggaaca gcgtcagcac
     8341 gcccgtaagc acggtgcgcg ccaggcgcgt ctggcgcatg gaagccagtt cctgggcaac
     8401 cgccggcgcg tcggacaggc ggttgtattc gcctatctct tcctccggtg ccagcgcgcc
     8461 ggagtttttc ctgcgcagcg gctcgtattc gcccgtgccg tcgccgtcca gcgggatatc
     8521 gatgtccatt gcgcgggtat cgcgccgctt gcgcgcagcg cccaggtcga atgtgtcggc
     8581 tctggcaatg gcccgcgcca aaaaggaggc ggacataggt gcgggcgtgt cctgcgggtt
     8641 tacctggggc acgtccagat ccagcgtgct gccgaaagtg cgcccattgg ctccgggcgc
     8701 gccctgctcc ggcgcatccg cccggcccgg cacgggcgcg cccggaatgc actgctcggg
     8761 gcggatggcg tcaaattccc cggtatagcc ctcctccaga gcggcctcat agtactcgcc
     8821 tgcctcgccc tgggcaaact catcctcgaa ttcctcttct tcttttttgg caaacagctt
     8881 cgcccagaag ccgctctttt cttttttacg acggccgctc tcacccagcg tagggtcgtc
     8941 gatcacggcg gttgcgaaga aatcgcggaa acggtcgtct acttcgccga acagattatt
     9001 tccggtaacg ggggcggcct gctcctgttc ctgcgcaggc tgggagccgc gccggagcac
     9061 cgcctcccgc gccgcgctca gcacggcttc acccgcggca ctgtgcagcg tattttccgc
     9121 ctcgcggcgt gcctcttcca taatgcgcgc gcgttcctcc gcctgctttt gcgcctcggc
     9181 ctcggaatac gccttaatgt ccggcagctc caccgtgggc acttcgtcct caacaggcgc
     9241 gggctccggt tcaggctccg ccgcgggttc cggcgctttc tcgggagcgg gctggggaat
     9301 ctccggcagc ggatcgtttt tttcgtgggc aaactcaggc accggcagca agatcggctc
     9361 taccggagcg cgcttttttc cggtcccgac gcccagttcg gataaaatgg cgtccaggtc
     9421 cgcatccgaa ctctgtgccg gacgggctgc cgcgggcgcg tccgcccgct tcttctgcac
     9481 ctgctcgagg atatggtcga gctctgaagc tgtgatatgc ggtttctgat cgctcaaata
     9541 gaaccctccg ttccgcgctg gcgcataatc tcatacagaa taatgccggt ggcgaccgac
     9601 acgttgaaac tgtccacccc cgtgccgccg cccgccatgg gcagactgag aacaccgtcg
     9661 cacagggact tcaccagcgg cgaaacaccg ctgccctcac tgcccgccac cagcgcgatg
     9721 gggcctgtaa ggttttgctg gaaagcgggg cgtccgtcca tgtccgcgca atagacaaat
     9781 acattctgcg ccttcagcct gcggacagcc tcgccgatgt tggccacacg cgccacgggc
     9841 agccttgccg ccgcgcccgc gctgcttttg agtacagtag gtgtaacagg tacgccgccg
     9901 cgccgcggta tcaccacgcc gtgagcaccg cacagcagcg ccgtgcggat gatcgcgccc
     9961 aggttatggg gatcctccac gccgtccgcc agcagcaaaa agggcggctc gccccggcgc
    10021 gccgcctcgg ccagcaagtc atccagcgag acataatcca cactggccgc ccacgccgca
    10081 acgccctggt ggccttccgt gccgcacatg ccgcgcagct tggccgcgtg cacacgcttg
    10141 accacagcgc ccgcctcgcg ggcgatggcc gtataatatc ccgcctgtgc ggcgttcatg
    10201 gtatcctgta cgaacacggt atccacggcc gcaccgcttt tgagcagctc cgtcacggca
    10261 tttttgccgt acacaattgt ggaatcctgc tctgttttgt gttcaaatct atcgtttttc
    10321 ttctctgccc gttccatccg cagcctccag aattttcaaa aaagccccgc ccgtccggtt
    10381 cccgcacact gcgctccgca tgcggcgtgc gcgcagccgc gcaagcggcg ggtttgaggc
    10441 tattataaca cagataccgc atctttgcta taatttccgc cggatttcgg gcattttcgc
    10501 ctgagcctga cagcagcgcg cttacaaaag gaaccacagc agcacagcgg cggccgtggc
    10561 agccgctgcc cacagcgcgc gcgcccggtc ccagcggcaa aatactgcgc cgccacgaaa
    10621 ccaggccgga aacatatttt tccgccgcat cctgctggca ccccaactgc accgcgcggc
    10681 tttccggctt aaaaaacacc agcacccgtt cgatgctctc gtcctgcggc tccacaattt
    10741 ccaccacaca cttgtttacg cctttcattc cggtccccct ttcccgcccg cctgccgcgg
    10801 gcttacggaa agttttgtct ccggtgcgca atttaaacaa tttcttcttt taaatttcgg
    10861 cgttcactgt ttaatggaat tcgtaaaggt gcatatacat tttgtaaaag gtaatttttt
    10921 ccttattgaa tgttgaaaac cctgttgaaa gtgttgagaa cccgcctgtt ttgcccattt
    10981 gcgggctttt tgaga
//
LOCUS       DAWBSB010000061        10934 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1393_length_10934_cov_3.348561, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000061 DAWBSB010000000
VERSION     DAWBSB010000061.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 10934)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10934)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10934
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1393_length_10934_cov_3.348561"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    8222..8231
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2141 a         3426 c         3144 g         2213 t
ORIGIN      
        1 cgcagctttt tcgacaagct gaagcgccct gcgcgccatt gggcgcgcag ggcgcttttt
       61 ctattccagc tcaaacagcg cgtcctcata atcctgcaca taatagcgga tattcgtgcg
      121 gccccgccgg atgacggtgt gcccctccgc ctccagacgc gcacgttggg cctccgcgcc
      181 gcccggatat tttgcgttca gctcgccgtc cgctttcagc gtgcgccagt aaggcgtttc
      241 gtctgtctgc cgctgggcgc tggcccatgc cgaaatggat acaaaaatgc ccgcagtgat
      301 ggggtcggta aaatcagcgc cgtttttccg ggcgaaatat tcccggatct tccctaccgt
      361 gagtactttt ccgaaaggca cccgcttcat taccgcgtca taatccgccg ccggggcaaa
      421 gtacatccgt gctccgccgt atttttgcac tgtggccgcg tccgtcacca gctgtatcct
      481 gggcatatcc gtggccctgt gcagcatcgc gttgaaatct ttcttatcct cgttcgccat
      541 tgcgaaacca cctcctatgc cctcagcata ccgtatcgga gccggacatg caatgaggcg
      601 gtttgatttt tacggcagaa tttccacata ataaagcacc gggcagcagc atgcgccgcg
      661 gcccggtttt gccgaatgtt tcagtcgctc agtggctgtt cagcacgcgg tcgatggttt
      721 ccatcgcccg ctccgcgctg gcgcccgcct ttttcatcat ctttttcatt tccttcgggt
      781 ttttctgagc cacatacgcg cccgccgcgc ctaccgcgcc gccggccagc agccctaagg
      841 ccgcgccctt catcatactc tctgtatttt tgcgcatatt tatcctcctc gttttattat
      901 cttttgtatg cattgccgcg gcttgtgccg ctttattgct agtatgcccg ccggtgcgcc
      961 aaaatgcggg ggcaaatttt tccacttgtt cataaaaccc cctcttattc cggcaaaaaa
     1021 tatgctatac taaagaataa ccgcaggccg ccgtctgtgc ggcccggagg aaaggagggc
     1081 ccgcatgcaa aaacagcata ccgttctgtg catacaggat ctctcctgtg tgggacggtg
     1141 cagcctggcc gttgtgctgc ccgctctggc cgccatgggc gtgcagccct gcgcgctgcc
     1201 cacggctctg ctctccaccc acaccggagg gcttggcagg cccgcgcgca cagatgagag
     1261 cggatttatt tccgccgcac tggcacacta tgcgcagctg ggccttgcat ttgactgtgt
     1321 atacagcggc tatcttgccg gcgccgcaca ggtggcgctg gtggaacagg ccttccgcca
     1381 ggctccggcg gcgctgaagc tggtggaccc tgtgatggcg gaccacggca ggccctacgc
     1441 ctccatgacg cctgaaatac gcgccgccgt cgcacaactg tgcggacagg cggatgtgat
     1501 cgtcccaaat gcaacagaag ccgcgctcct tctggaggag cttcccggcc cggcggcgga
     1561 cggaacgcgc gccgcccgcc atgcatccct tttgctgcag cgctttcccg cgctgcgctg
     1621 cgccgtggta accggcggcg ctctttcgga cggacagcgg ggcaacgtgt gcgctgtgcg
     1681 cggcagggcg cccgttttcc tgccctattc ccccgtcccc caagattatc ccggcacggg
     1741 cgatctgttt gccgccgtgc tcaccggcgg gctactgcgc ggcttttccg aagccgccgc
     1801 catccgtctc gccgcgcgtt ttgtggcgct ggcggctgaa cagacccttc acgccggagc
     1861 ggatccccgc ttcggcgtgc ggttcgagcc gcttctcgga acgctttccc actgggccga
     1921 aacgcctgca cagccctgta cggacgctgt ttttccgctg taaaaacacc agacagaagg
     1981 agcgcaatct cacccatgaa accgttgaat atcgtgctga tggagccgca gatcccccaa
     2041 aacaccggca acatctcccg cacctgcgcg gccacgggcg cgcggctgca ccttgtgggg
     2101 cccatgggct ttaccgtgga tgacaaaaag ctcaaacgtg ccggccttga ctactggcac
     2161 ctgcttgata taacatatta tgaagggctg acggattttt ttgcccgcaa tgaaggtccg
     2221 ttttactatt tcaccacaaa ggcaccgcag cgctacacgg acgtcgccta ccccgacggc
     2281 gcgtaccttg tgttcgggcg cgaagacgcg ggcctgcccg aagcattatt ggtagaaaac
     2341 caggaagcct gcgtgcgcct tccgatgatc ccggacgcgc gcagcctgaa tttgtcaaac
     2401 acggtggctg tgggcgtata cgaggccctg cgccagcgtg attttgcggg tctgctcgac
     2461 accgggcgcc tgcgtggata tgaatggaag tgaacaagtg agtaaaacag ctgttttgac
     2521 ggattccgcc agcgacatcc cacgcgagct ggaagaaaaa tacggcattg atatcctccc
     2581 gttcagcatc gtggtggacg ggcagagcta taccgaacgc gtggatttca ccaacgaaca
     2641 atattacgag atgctcacca aagccgaagg catccccaaa acatcgcaga tcacgatgct
     2701 gcgctttttg gaaaagtttt gcgcgtatgc cgacgaaggc tatacggatg tgatcttcgt
     2761 gtccatcaat tcggccggtt ccaacaccta caacgcggcc tgcatggccg ccgagagcct
     2821 gcgcgacgaa cgccccggct gcacgatgaa cgtgcatgtg gtggacagcc acacctattc
     2881 catgacatac ggctggcacg tatgcgaggc cgcgcgcaag ctgcaggcgg gcgcggacgt
     2941 aaaaagcgtc gtggaatatc tggaggacca gctggcgcgt atggagatca tgctctccat
     3001 gtatacgctg cgctttgtaa aaaagagcgg ccgtgtctct gcggcagccg cattcgccgg
     3061 cgagctgctc ggcctgcgcc ccatcatcca tatggtggac gacaccacct ccaccatcgc
     3121 caaggtgcgc ggcgacagcg ccgtgatgcc cgggctcatc gtccagacca aaaaacggat
     3181 ggcagagggc acgccttatc tcatcggcgc aacggacgat ggcaacgcca aaatgctggc
     3241 caagatgtgc aaaaaagaat tcggttatgc gccggccgcc actttcctgc tgggagcggc
     3301 cgtcgccacg aacaccggcc ccaatgccgt ggccatcgta taccagggtg aaaaacgccg
     3361 ctgaaccatc ctttttccgg atgaacaagc gtccgcgccc cataagaaca taaaaggaga
     3421 acgtcccaat gggaaacccc atcctgtata tcgtgattcc ctgttacaat gaagaggccg
     3481 tgctgcccct gaccagcggc atgttcttga aaaaaatcaa agaccttgcc gcagcgggca
     3541 agatatcgga caaaagccgc atactgttcg tcaacgacgg ttccaaggat aagacctggg
     3601 acatcatccg aagcctggct gaagcggacg agcattacag gggcgtctgc ctttcccgta
     3661 accgcggcca tcagaacgct ctgctggcgg gcctgatgac cgccaaggac ctggccgacg
     3721 tgaccatttc catcgactgc gacggccagg acgacatcaa cgccatggac gcgatgatcg
     3781 acgaatacct ggcgggcatg gacgtggtgt acggcgtgcg ctccaagcgc gagacggaca
     3841 cctggttcaa acgaaccacc gccgagggct tttacaaagt gatgaaggcg ctgggagcgg
     3901 atgtggtgtt caatcacgcc gattaccgtc tgctgagcaa acgcgcgctg gagggcctgg
     3961 ccgagttccg ggaagtgaac ctgttcctgc gcggccttgt gccgctggtg ggctaccgct
     4021 gctccagcgt atactacgag cgcgccgaac gcattgcggg tgaaagccat tacccgctga
     4081 agaaaatgct tgcctttgcc ttcgacggca tcaccagcct ttcggtaaag ccgctgcgcc
     4141 tcatcacgct ggcgggcatg ctggtcagcg ccgtcagctt tatcgccatc atcgtcacgc
     4201 tcatcacaaa gctggtgggc tttacggtaa cgggctggac aagcatgatc tgcgccattt
     4261 acttcctggg cggcgtgcag ctgctgggga tcggcgtcat cggggaatat gtgggcaaga
     4321 tctacaacga aacaaaagcg cgtccacgct tcatcatcag cgagacgacg tttgaaaagg
     4381 agaagccatg agcaagcagg tctggaaagg ctcggcgctc gtcgcgcccg tgccgcccgc
     4441 gctggtaagc tgcggtacgc tggaggcgcc gaacgtgctg acggtggcgt ggacgggcat
     4501 gctgcagtca cagccgccca aaacctatat ctccgtgcgg ccggagcggt acagccacgg
     4561 cctcataacg gaaagccggg aattcgtgat caacctgccc accgccgcgc tggtgcgcgc
     4621 catcgactat tgcggcgtcc gaagcgggcg ggacgaaagc aaattcgaag ccatgcacct
     4681 tactgcgctt gccgcttcgc aggtttcctg cccgttggtg ggcgaaagcc ccgtgaacct
     4741 ggaatgccgc gtgacagaca ttgtaaagct gggcggctcc catgatatgt tcattgcgga
     4801 catcgttgcc gtgaacgtgg acgaggagct tctggacgaa aagggcaggc tgcgcttggc
     4861 caaggcgggc ctgtgcacat atgcccacgg cgaatatttt gcactgggca aaaaaatcgg
     4921 cgatttcggt ttttccgtca agaaaaagaa gaaaaagccc cagtacgaca ggcggaaata
     4981 aggccgtccg cctgtgcaaa ggcaattccc tgttcacctg aaaagaagaa aaaacgcgcc
     5041 ccggtacggc tgaaaagcga taccggggcg cgttttatat gcatgcttca accgcgcggc
     5101 gccgtttttc cgccgcccgg aaagcacagg cggaattgaa gcgccgcttg ctttgcgaag
     5161 gaaaacattt ctgatgcaaa gcagcgttcg ttccgcagct ttcaaacaaa cacaagctgc
     5221 accagcagca tgtacagtac cgtacccacg cccacgctca aaagcgtgtt ctttttccaa
     5281 acatgcaggc ccgccgtcgc cgccacggcg atcagctccg gcaggccgta gggcgcggcc
     5341 gccggctgca catccttcaa acagtacacc accaacaggc ccatcacagc acagggcagc
     5401 acctttccca gatatgccac gatgcgcggc gccggtttcc ccttgggaaa cagccaaaac
     5461 ggcagaaaac gtgtgaacac tgtacccagc gcgattatcc caatggtgat caggctttgt
     5521 gccgtcgtca tcgtaccgcc ccctttttgt ccagtgcggg ccgcattaca agcagcagca
     5581 atacaataag cgccatcgcg ggcagaataa aattccccgc cccgaacaac gcgaggcaca
     5641 gcaccgatgc gcccagtccc accagcgccg ggcggtgctc tctgtcttcc atccactggc
     5701 ctgtaaaaat ggtaacgaaa agcgccgtca tcacaaactc aatgcccttt gtactgacag
     5761 tgagcacgcg gcccagcagc ccgcccagcg cggacgccgc cacccagtaa aattggtcca
     5821 gcagggacac ccagaacatg aaccagccgc ggtccacgcc ttcgggcggg caggcggaca
     5881 cattgacgga gaacgtctcg tccgacattg caaataccag atacggcgtt ttctttcccg
     5941 cggcacggta cggctccagc atggatacgc cgtagaacag gtgccgcgcg tttaccatca
     6001 gcgtcagaaa aaacgcgccc gcaggatcga atgccgcacc cagcagaggc actgtcgcat
     6061 actgcatgct gcccgcgtat accaccaggc tgatgagcgc cggataccag aacgggaacc
     6121 ccgcgctctg catcaaaatg ccgtacgcca gccccaaaaa cgcatagccc gtcagcacgg
     6181 gcagcgtgtg gggaaacgcc gcccgcaatg ctttcctctt cataatctct tgccctcccc
     6241 ggaccgcgcc aaaccgcgcc gcccttcgca tgcccttttt cgggcacata tttggaaata
     6301 attatagcac agcgcgcagg tttgtgctat actatgcgtt gcggccctgc gccgcacagc
     6361 aggccgtttt gcgccgcgct tttacaggcg cgaaacagcc tgacgggcaa cttagaaggc
     6421 tgtgccttcc gtgggatttt gtgcccgccg cgcttttgcg cggaccaccc ctccggcccg
     6481 gcggcaccgg tggggtaaaa tactgccgct aaggaaaact gtaccatgaa aaaaacaaaa
     6541 accagaacca tcgtccgctg cgcgctcatt gcgtcgctct acaccgctgt gagcctcgtg
     6601 ctcgccccca tcgcctttgg cgccgtacag gcccgtgtga gcgaggcctt cacgctgctg
     6661 cccgtgcttg tgccggacgc cgttgtgggc gtcacgctgg gctgtttcct cacgaacctt
     6721 gtgggcgtgt ttaccggggc aaacgtgctg ggcgcgctgg acatcgtatt cggtaccgcc
     6781 gccacgctca ctgccgcgct gtgtacgcgc cgccttgccc gtgtgcgcct caggggcctg
     6841 cccgtggccg cggctgtgcc gcctgtgctc atcaacgccg ttgtcgtggg cgcagagctt
     6901 gcgtgggcgt tcggcccgcg cacctttgcg ggctttttgc ttcaggccgg cggtgttgca
     6961 ctggggcagc tcttctcctg cttcgcgctg ggtctgccgc tggtgcgcat cattgaaaaa
     7021 acgcccgcgc tgcgggcgtg gttccgggat tgaacggttt tttccgcttg ttccgcgaca
     7081 aaagcttatc ctgtcggccc gtatggccga accgacggcc cgcggctgtt tcgcattttt
     7141 accgaccaag gagtgagacc atgcctgttt tcgacacggt cctgtgggat tggaacggca
     7201 cgctgctgga tgacgccgcg ctgtgctgcg agcttttgaa cacgatgctt gcccgccacg
     7261 gttatgcgcc cgtgggcagc atggaagcct accggcaggt cttctgcttt cccatcgaga
     7321 cctattaccg ccgggcgggg ttcgattttt cccgtcatcc gttcgccgca cttgcggatg
     7381 aatacatgcg gctgtacacg ccccgcagcc ttggctgtcc actgcagccg gatgcctgcg
     7441 ccgtgctgga cgcgctgcgt gcgcagggca tgcgccaagt gatgctctcc gcctccaaac
     7501 aggagaatct gcagcagcag gtggagcatt tcggcctgcg ttcccgtttc gacacgctgc
     7561 tgggcctgtc ggacatctat gccaaaagca aaacggaagt gggcctgcgc tggctgcggg
     7621 aaagcggcgc ggacccggcc cgcatcatga tggtcggcga cagcgagcat gattttgaag
     7681 tggcccgtgc gctgggcgta cgctgcgtgc tgttttcggg cgggcaccag ccacgggaag
     7741 tgctggccgc aacgggcgca cctgtcatcg acgcgctggc gcagcttttg cctctgctgg
     7801 cacagcttgc agcagaataa ccgaaaacgc cgctgtccgg gtcgatcccc atccggacgg
     7861 cggcgttttt tgtggcgcgc ggtgcggcgg cgtgctatac tgatagtgta cccgccgccc
     7921 gccgggcggc tcagacggaa aggatgggat aggatgaacg aagctcttgc ttatgtggtt
     7981 gcgcagcgca tggagcgcac tgccgccgcg ctgcgaaaaa acaacatgga ggccatttgc
     8041 gtggacagcg cggcagaggt gctgcccctg ctggaacagt ggattgcgcc gggttcttcc
     8101 gtgggcaccg gcggctctgt tacgctggca gagtgcggtg tggaacagtt tttgaacagc
     8161 ggcaaagtaa aatatctgaa ccggaatgcg ccgggcctca cgccggaaga gcgggaggaa
     8221 gnnnnnnnnn nggcctattg cgtcaaaacg gccgacgaag tggtgcctct tgtcaaaacg
     8281 ctgttggcgg aaggcgcgac cgttggcgcg ggcggcagca tgacgctgga ggaatgcggc
     8341 gtgatggatc tgctgcgaag cggcgcatac cattttcttg accgtggcgc tcccggcctg
     8401 acgccggagg acattgggaa gatctaccgg cagatctttt ccgccgactg ctattttgcc
     8461 tccgccaacg ccgtcaccga ggcgggcgaa attttgaatg tggacggcaa cgccaaccgc
     8521 gtggccgcca ttaccttccg ccccgccagt gttatcctcg tggtgggctc caacaaaatc
     8581 gtgaaagacc ttgccgccgc cgacgcccgt gtcaaggccg ttgccgcgcc ggcaaatgcc
     8641 aaacgccttt cctgcaaaac gccctgcgcc gttacaggcc agtgcgaaaa ctgccaaagt
     8701 cccggccgca tctgctgcac atacgtgctg caccgctacc agcgcgtgcc gggccgcatc
     8761 aaggtcatcc tcgtgggcca ggagctgggc tactgaaccc gaacctcaac ggcaaaaaga
     8821 gcgcgtgctt ttcagcacgc gccctttcct gttttcaatc agtaacctgc cctgccgtct
     8881 gcgccacacc aaaacctacc gaaatggcag gtgggtgccc tgccggagtt tcacgctccc
     8941 ttcctccgct tgcggccaag gccgggcggg aggtctgcga tcccctcaca taggactccg
     9001 ggctcccttt aaatggttag taggattaaa tatatggcgc atcaaatcgc acagggcagg
     9061 ctgcctgccg gtttattggc cgccgttgag gtcgatgtcg tacaactcct ccagacggcg
     9121 cgcgaacatg tcgctcacct cgtaaagctc ctcatcgtca tccacaacgt ccagatattc
     9181 ttcgccgtcc tcttcgccca cgcgcatgat gatgaggttt gcgtcctcct ccagctgctc
     9241 cgtggaatcc tcggcatagg gcaccaccgc caggtagcgc tcgtcgttgt aatcggcggc
     9301 gtcgatcact tcaaagctga actccttgcc gtcttcatcc gtcagggtga tgaggtccgg
     9361 ggagtaatcg tccagttgtt cgtcctgagg catgggaatg atctccttca ctgctcgtcg
     9421 ttcctgtttc tgtttttatt gtacccaatc cggcgcccgc cgtcaagcat gccccgtaaa
     9481 tttacagttc attcacttct ttccctccgg gtttgtgata aaataatttc aaagccaaag
     9541 cccgcgcgca catgccgtga acgcgctttg cgctgcggcc tgcaagctcc gttcacactg
     9601 ccgcgcaggg cggcggagcg taaaaaacat tcttttatct ttatccggta tccgggcgtt
     9661 tgcgcccgga taccgccgca cagggggaaa cagccttgac caaacgtatc tttatcacct
     9721 tttgtgccgc agccgcgtgc tgcgtgttgc tgggcgcctg cgaaagcggg agcctcgctg
     9781 ccgccgcggg caagcgcacc cgtcccgcag accttggcga gctccagttc atcacgcccg
     9841 ccgaaggcga ccccatcgct acgctctcca caacgctggg cgatatttct tttgtattgt
     9901 acccggatat cgcccccatg gctgtggaaa attttgtcgg tctggcccag cagggctatt
     9961 acaacggcct gtccttccac cgggtgatcg agggctttct ggtccagaca ggcgacgcca
    10021 caggtaccgg cgctggcggc tccaccattt ggaacggaaa ttcttatccc aacgagatct
    10081 ccgaccgtct gcaccattat gccggtgccg ttgccatggc gcacgccccg gacggtgctt
    10141 cgggcaacct gagccagttt tatatcgtgc agagcgccca ggattccgtg gataagactc
    10201 tggccaaaac gctgaccgag gcaggtgtgc gcgaggccgt ggtaaccgcc tatcaggccg
    10261 tgggcggcgc gccttatctt gacaacctga acaccgtgtt cggccaggtg tacgacggca
    10321 tggacgtcgt ggacgccatt gccgccgtgg aatgcggcga agatgaccgc ccgctggaag
    10381 acgtcatcgt caacagcgtt accatcggca cttattccgc cgcgcagagc ggcagcacgc
    10441 ccgcatccgg cgcatccggt tccgacagcg ccgcctgaat cgccccatac aaaccttaca
    10501 aggcgcgctc cgcattgcgg ggcgcgcctt tttaccggca gttttcccgg tcactcccgg
    10561 caaagcctac tgcattacta ttttttttgc gcgaaatatg gtacactata aagcatcggg
    10621 acaagcgctc tcttgcgtct gtgtggaggt acggtgtgaa aaagatactc aaatttctgc
    10681 tctcccggac attcctgttc gggcttctga tcgcatgcca ggtggggctt tttgcggcgc
    10741 tcatgctggt attcagccgc gcgggtacgg tggcttacat catcctcacg ctggtcagcg
    10801 tgctggtggt catcgccgtg atcgaacgcg acaacatcaa tcccgcttac aaaatcatgt
    10861 ggctgctcat tgtggtggcc ctgcccgtta caggggcggt attctacctg tggtggggca
    10921 accagggcgt cacc
//
LOCUS       DAWBSB010000062        10652 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1438_length_10652_cov_3.530811, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000062 DAWBSB010000000
VERSION     DAWBSB010000062.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 10652)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10652)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10652
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1438_length_10652_cov_3.530811"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    9352..9361
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10360..10369
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2015 a         3374 c         3166 g         2077 t
ORIGIN      
        1 ctgcttattg ggcccagccg gatttctcgg ccagagcggc ggcgaaatca ttgagggctt
       61 cctcagccgt catgtcgccg ttcaccgcct gcttcagagc atcgcggtac tcattgtcca
      121 gatagtttct ccacatggag gattcgtcca cgctgaacag ggtagtcgtc tggccgactt
      181 ccgcctgcag gaccgtgtag tccgcggcag ccttctcgtc ggcgggctgg tacgtcacgc
      241 ccgtcagaga ggacaggccc ttgtccagat cgaggtagta agagaagttt tccttctcat
      301 agaaccactt gatgaaggag agcgccagat ctttgttggc ggagttctcg ggcacggcca
      361 gaccggagcc gcccagaatc gcggaaccgc tcacagagcc gaccggagaa ggcataacgc
      421 cccattcaaa gccggtgatg tccgtggtga atgcattgta gttccaggaa ccggagaagt
      481 acatgcccac gtcgccgttc ttgaagtaat cgccggggtt gtcggtggag ccgccggccc
      541 agatggcctt gggcataatg ccctcgttgt tcttgtccac gaaggtctgc agggtgtcga
      601 tattggcctg gctgttgatg gcgaccttga aggaatcgcc gtccttttcc accatggagc
      661 cgccgttcat atacatcagg ttgtcataac gggcgcggga gaaatccacg gccatgccgt
      721 acttcacacc gccgtcggcc atcagcttcg cagcggcctc gtacacttca tccatcgtcc
      781 agcggtcgtc cacagtggga acctccaggc cggcggcctc gaaagcgtcc ttgttgtaga
      841 acacgttggt gatggtgaac tgcaggggca ggcaggagag cttgtcggag ttatattcct
      901 ggctgatgat ctccagtgcc atcggctcga agatcgaggt gtcgacgacc tggcccaggt
      961 ccacaaactc atccggatac agacggctca gacgcgtggt ggaaacgagg tcgggcaggt
     1021 ccttgttctt ggccatgagg gggaacttgg tgagctggtc gtcgtaggga atgaccatga
     1081 gctccacgct gttgccggtc tcctcggccc atttattcag tgtcgtggtg aaggaatcgc
     1141 cgctgctggg ctcctcggaa agcatgacct tcagcgtctg gcccgtaggc gcctcggcag
     1201 agccggacgc cgccgtggaa ccggaagccg caggcgtgct gctggagctg ccgccgcatg
     1261 cgacgagcat gagcgccatg gcggcaacaa gaagcaggga aagtaccttt ttcatacgtt
     1321 aaacctccta aagattaccg ctgcgccctg cgggcgcagg tttcttcctc aaaacagcgg
     1381 gacgcgcatc tcccgggaag catgtgccgc cgccgttttt ctgtgcctta agtatagcat
     1441 tcgccctttc gcggaaacta ctcctatttt gctgtgttgc ttcagattgt attgcatttt
     1501 ttgctttgga tttttcatct ttggcaaaat gcatcaagca ttttgttcaa aattggtaat
     1561 aatcccaatc aaaatctatc aaatctgtgt gcgttttgca gcaaagcgcc tgccaaagcc
     1621 cgttccacgg gaaaaggcaa agaaaaaaat gcaatatcgc gttgtcataa tttcgtaacc
     1681 tttctatgtc cctacaaata aaagacgcaa aagcggagcc gcggcaggcc ctcttctgcg
     1741 tcttttgtgt tttttatata aagtaagtta tattataaac agagatccgc ctgctccccg
     1801 gcgcacctgt ccggatgcgg catggcgtgg ccgaacgcgc cgtaatggcc gcagcactgc
     1861 gcggcaaata cgctcgcctt ctgcgctgcg tccgccagat ccccgccgcc cgcgtggtat
     1921 tccgcaagaa atgccgcaat gaacgcatcg cccgcgccca gcgcgtccac cacgtctgca
     1981 cgcacacact cctgcctgtg ctcacgcccc tgctccagca gataagaacc gcgcacgccc
     2041 atcgtcacca cgaccgtgcg cgctccggcg tccagcgcag tgcgggccag cgcccgcacc
     2101 tcttcctcgc tggcggcgcc gccggaaaag aaagcaaacc gcagcagcgg gcagagctgg
     2161 gcgatgttca cccggtcgta ctcgccggaa aagtccagag agagcggcac ccggcggctg
     2221 atggccgggt ggtaggcatc cagccagctg tggatgctgg tgtgcgccag gtcgtactgc
     2281 tccatcatgg cgtagtcgcc gggcgtcagg cacagcagcg cctggtactg cgcggtctcc
     2341 cggccgttgt tgcccacaaa gtggcggtcg cccgcctcgt ccagctcgat gtaattgcgc
     2401 gcgctgacgc cgtgcatcac acgcacgcgg ctgatatcca cgccttcctc ccgcagggcg
     2461 gaggcaaaat gcagccccgc gccgtcatcg gccagcacgc cgatataggc tgctgtgctt
     2521 ccgtcgtagc ggcgggccag cacgggcaca ttgacgctgt tgccccccgg gtacagctcc
     2581 tgccgccagc ggtaatcgtc cagcacgttg tctcctatgc caagcagctt catcagtatt
     2641 tttccacccc catgtaccgc cgcgaggaaa tagggtgtcc catcgcatag gacatctcgt
     2701 cgcaatacag cgcggccaga cggttgagca ccagtgcgga gaccggcgca cgcagccagc
     2761 cgggataagc ctcgagatcc agcgcggccg cgtccagcac cgtcagcttc ccggtcttgc
     2821 gctgtaaaaa cttcaccgcg cgttcctcca gggcccgcgt tttgcccaga cccagcagcg
     2881 caaacaccgc gaccgtatca tccacggcct caaacgcacc gtggaaaaac tcgcccgcgt
     2941 gcagcggcga ggagtgcttc cacagggatt ccatcaccag gcagttcgtc agcacatagc
     3001 ctacgcaggc gtccaggccc gccgcaatgg aatagaggat gggttcccca gcgtattttg
     3061 cgccgaacgc ccgcgccgcg gggcggtagg ccgcgcacgc ttcgtcgaag gtatcctgca
     3121 ggcgcagcat ggccgcgttc acttccggca ggcggcccgt gccgttccac acatccagca
     3181 gcgcgtaggt cagcttgtat acctcgggaa agatcgtcag caccgcggga tacggggacg
     3241 ccgggtcgtc gaagtaataa acgatatggt ccgccgcctg ctccagagcc gaaccgggcg
     3301 tggtggtgaa cgccgccgtc agcgcgccgc gcccggtggc cagccgtgcc gccgcggcgg
     3361 tctccggcgt gccgccgctt tgggaattga gcaccaccag gctcttttcg cccaggcgcg
     3421 cggggggatc gttcaaaaac tccgccgccg tataggcccc ggtgctcacc gccgccgttt
     3481 cgcgctcaag gatatacttg cccgggtaca gcgtggccag cgaaccgccg caggccacaa
     3541 aatagacgtt ttccacagcg cgggccgcca cagcctgctg cactgctttt acctggccgt
     3601 ccaccagtgc gatcttttct tcatgcatcg ttcctctgct cccttgcttt ttgattttgt
     3661 ccgtatctgc atttgcactt caattactaa agtttgttta gtaatttgtt ttcactatag
     3721 cataacgcgg ccgggagcgc aagccctcct gcaaaaaaat ttattctcca ccccgcggcg
     3781 gccgctttcc cctttgaccg ccgggcacag ggatggtatg attatcataa cttttggata
     3841 tctgtgcgcg ctgcagcgcg cgggagggcc gtatggaacc atatcttccg aattatctca
     3901 agggaaaaaa ccgccgcctc gtgttcgacc tgctgcgtga gcgcggccag atgagccgcg
     3961 cggagatcac ccgggacacg ggcatgagct tccccaccgc aatgaaggta gtggacgcct
     4021 ttctggaaaa gggcctgctg gtggagctgg atgaaaccgg ccccgcggcc ggcgccgggc
     4081 ggaaagggcg cctgctgcgc tttgagccgg gagcttaccg cgctgtgggc gtggaatgcg
     4141 agggacgctt tgcccgcgtg gggctggcgg atatggcggg cggcgtgcag gcgcgggaaa
     4201 ccatcgagct gggcgatttc gcacacacgc gggacctttc cccccttgcg gcggccatcc
     4261 ggcgcatgtg cgctgcggca aacggcgctc ctgttctggg cgtgtgcatc ggctttcccg
     4321 ccaacctgga cccggacgcg gcggccatcg tcagctacgc ggccatggac atccagcagc
     4381 ccacctcctt tgcgcaggta ttccccgcgt tctggcggga agtggagctg cccgtctatc
     4441 tggaaaacga tgcgaacctc gcctgcgagg gcgaggcatt cctgcggcgc ggcgcgggcg
     4501 gagcggccaa tatgctttat ctgtccatcg gctcgggctg cggcggcgcg gtgcgcctgg
     4561 acggaaaact gcgccgcggc gcgcgctgcc gcgcgggcga ggtaggcgcg atgcttctgc
     4621 agccggcgct gggtgcgccc gggcgcagcg ccgcgcggga aacgctggag gacgccgtgt
     4681 gtctggccgc gctggagcgc cgcttcggcg cagcgctgcg ggacggcgca cagccgggcg
     4741 aagccagcgc acaggccatg cgggactatc tgtgcggccc gctgagcggc gctgtttaca
     4801 gccttgtgat gacgctggac gtggagctgt gcgtgctggc gggcatcgtg cccggcgccc
     4861 tgggcatgca gctttgtact caagtgtccg aagcgctgcg cgccgccctg cccgagagca
     4921 cgcccgtgcg cgtggagcct tcggtgagcg cggacgcggg cgtcatcggc gcggcggcca
     4981 cggtgttcga gcgttctctg gacgccctgc tcggcagcgg ctgacggcgg agcggcacgg
     5041 cgcgggcttg cgcaggggtg cgccgcaaag caacgaatgc aagcttgccg ccgcaagcaa
     5101 agcaaaaaac ggatagccag gcgctccggc gcgtgctata atgggtgcgc ctaaaacacg
     5161 gcggatgcgc cgtggccgcc gcgcggcggc gggatggaac ggaacagccg gggcgcgcac
     5221 cgcacgcccc gtgagaaaga gagggaaaca catgacgact gaacaactgc gccaccgcat
     5281 gcctcccatt ttgaaggcgc tgaaggaacg ctccctgcgc gggcgcacac cggtggaggg
     5341 cctgcgccgc accgaatgcg cctaccacgg ctgggacacc gtacacgccg atgccgcaag
     5401 ctgggagccc tttgcccccg gcgacgcttt cggagggctg gaggcgcacc actgcttcaa
     5461 aggcacggta acgctgcccg aggccagcgc cggaaaacgc gtggtctgtc tggtatccac
     5521 cggcgcctcc gatatctgga acaacaacaa tccccagttt ctggcctatg tggacggccg
     5581 gctggtatgc gggctggacg taaaccacaa cgagttcgac cttgcagcct gcgcggttcc
     5641 cggcgaaagc cacgagctgg cgctgtacgt ctactgcaac acacccgcgc gggatgtgtt
     5701 cctgcgcgtg gagaccgccg agcgcgacga cgacgtaacg gggctttact atgacctgcg
     5761 cgccccgtac gaggtgtgcg cgcttcttgc ggacgacgac acacgcgcca tcggcatcat
     5821 gaagcacctg aaccgcgccc tcaacctgct ggacctgcgg gacctggaca gcggagcctt
     5881 cgcccaaagc gtgcgggacg cgcgcgagta tctgcgcacg gaattttacg acggcttctg
     5941 cggccggaca gacgccaccg aagcctgcgt gggccacacg cacatcgacg tggcgtggct
     6001 gtggagcctt gcccaaacgc gggaaaaggc catccgcagc tttgccagcg tggattacct
     6061 gatggaacgc tatcccgaat acacctttat gtccagccag ccccagcttt acgatttcgt
     6121 caagcgcgac tgcccggccc tgtatgagcg catccgggcg cgcgtggccc agggccggtg
     6181 ggagccggag ggcggcatgt ggctggaggc cgactgcaac atgtcctcgg gcgaaagcct
     6241 ggtgcgccag tttttgcacg gcaagcggtt cttccgcgat gcgttcggcc gtgagaaccg
     6301 cattctgtgg ctgccggacg ccttcggctt cagcggcgcg ctgccgcaga tcatgaagca
     6361 gtgcggggcg gattacttca tgacaacgaa gctggcctgg aacgacaccg acatgatgcc
     6421 ccacgatgtg acgcactggc gcggcatcga cggcagcgag gtgctggctt atttcatctc
     6481 cacaaaagat tacgtcaaaa agccggacaa ggaccctaac ccctcgttta acaccaccta
     6541 caacggcatt ctcgcgccca ggcaggtgat gggctgctgg cagcgcttcc aggacaagac
     6601 gctgacggac gacgtgctgc aatgctacgg ctacggcgac ggcggcggcg gccccacggc
     6661 cgagatgctg gagctgcagc gccgccttgc ttacggcatc cccggcgcgc cccgcacacg
     6721 ccaaagcact tcgctggcgt tctttgaaga gctggaacgc cgccttgccg gacaggacgt
     6781 gccctgctgg tgcggcgagt tctattttga ataccaccgc ggcgtattca ccaccatggc
     6841 gcgcaacaag cgctacaacc gcctggccga gttcaaaaac gccgacgcgg agctgttcag
     6901 cgcgctgaat ctggcctgcg gcacagccca tgcctatccc gccgaagcgc tggcccacaa
     6961 ctgggagctg acgctgctca accagttcca cgatatcctg cccggatcct ccattgaaaa
     7021 ggtgtacgag gattccatgg agcagtatga gcaggtgctg gcctccgacg ccgcgctcat
     7081 cggcgatgcg caaaacgcgc tggccgcgct ggtgcgcgca gacggcgacg gcgttctggt
     7141 gttcaaccag ctcggctttg cacgggacgc gctggtgcgc gtgcctgtgg aagcgcctgt
     7201 cgcgggcgtg ctggccgacg gccgtcccct gcccttccgc tgggcggacg gcgagctgtg
     7261 ctttgtggcc gcagagctgc ccgccaaggg ctggcggcac taccgctttg ccggctgtgc
     7321 ctctgcgccc gttccctttg cgcaggtctc cgaagacggg cggcgcatta ccacgccttt
     7381 ctatgaagcg gagctggacg cctgcggcgc attcacccgt ctgtacgaca ccgcggccca
     7441 gcgcgaggta ctcaagccag gcgcacgggg caatgtgttc cagatgttcg aggatcgtcc
     7501 cgacaactac gacgcgtgga acctggagca atactattcc gaacacatgt gggagctgga
     7561 cggccctgcc gagctgagcg tggaagaaaa cagcgccgtg cgctgctgtg tgctggtaaa
     7621 gcgcgcgttt tcccgctccg caatggagca gcgcatcgtg ttctatccgc acacccggcg
     7681 catcgacttt atcacccatg tggactggca cgaggagcac gcgctgctca aggccgcgtt
     7741 ccccgtggac gtatatgcca cccgcgcgcg gtacgacatc cagttcggca gcattgagcg
     7801 ggacacccac cgcaacacca gctgggacgc ggcacgcttt gaagtgtgcg cgcacaaatg
     7861 ggccgacctc tccgaggccg gctacggcgt ggctttgctg aacgactgca aatacggctg
     7921 cgacatccac gacggcgtga tgcgcctgtc gcttctgcgt gcgcccacgc atcccaaccc
     7981 caacgcggac cgcggtgcgc atacgttcac ttacgcgctg ctgccccacg agggcgatta
     8041 ccgcacaggc ggcgtggtgc gcgagggcta cgcgctgaac tgcccggctt acgcccggcc
     8101 tctggccgcg caggacggcc cgctgccgga aagctactcc ttcgtctccg tggacgcgcc
     8161 gggcgtcgtg gtggaggcgg tgaagcgcgc cgaggacggc aatggcatca tcgtgcgcct
     8221 gtacgaggcc tggggcatgc gcacccgcgc cgttttgtcc gtgcccggca gcacgcgcgc
     8281 cgtcacgccc tgcaccgcca tggaggacgc ctgcggcgaa gcggcggtac cggaaaacgg
     8341 cggcatcccc ttccagatcc gtcccttcga gtttaaaaca ttccgcatcg agctcgcata
     8401 acaagcaaaa caccgggacc ctgccaaatg aggtcccggt gtttttttgc tctgcggccc
     8461 gtcatgcgtt gggcctgccg tttacggcac cacagaaaac agctctttcc aacgggaata
     8521 acttacctgc tttactatat tgccccaagg cggcaaagct gtgcggcaga attggaaaag
     8581 agcatggaag gccgctgttt ttcgccgaac agagccaaag ccgggaggcg ggaaaacaaa
     8641 ttttcccaaa accccacttt tgcatttatg cacaatatat tattatcata tttaggcaaa
     8701 ccgctgcatt tctttccctc ttcctgcctt ttcaccgtcg ataacgcaaa aaatgcaata
     8761 gagcagcacc aaaacaagca aaatagtagt agagcttgcg gagcagaaaa tgctatactc
     8821 aaaccacaga aatggggccc gcccgcagtg aatttatggt gataaagcac aacggccggg
     8881 aaaaactgaa attataggag gaatggcaat gctgaagaaa aaatcatcgc tgaggcatac
     8941 aggtgcattc tgggcctttg tagcgccctg ctgcatcctg ttcggcatct ttttcctgct
     9001 gccgctggtc ctgagcatcg cgttctcgtt cacgaactac gatggctgga aggtcatgga
     9061 cttcgtcggc atcaaaaact atatcacact gtttaaggat tccaaattct acgcgtccat
     9121 gggccgcacg ctggtgtaca ccatctgctg cctgcccttc cgcgtgatca tccctctgct
     9181 catcgcagtg ctcgtcacct cgccccgtgt acgcctcaag agcctgtccc gcaccatgat
     9241 ctacatcccg gtgctgatga gcgccctggt cgtcggcatc accatcaact ggatgttcag
     9301 ccaggaatac ggccttatca acttcatcat ccagtccttc ggcggcaccc cnnnnnnnnn
     9361 nctggaatgg tcgctcaact ccgggcttgc cacgttcgtc atcggcttcg cctccgtgtg
     9421 ggcctccatc ggcttcaata tgatcattta tgtgggcggc atcaacaaca tctcaccgga
     9481 cctgtacgag gccgccagca tcgacggcgc caacagcttc cagtctttcc tgcgcatcac
     9541 ggtgcccatg ctcagcccca ccacgttcct tgtagtgctg ctttccacgg taaacctgct
     9601 caaggaatac gctttggtac agggcatcac gcagggcggc cccggcctga gcaccaccta
     9661 tatcatccag tacatctttg acaagggctt caatcagatg gaatacggct atgcgtccgc
     9721 tatttccact gtcgtgatga tcgtgtttgc gctgatcgca tacgcccagt tcaaagtcaa
     9781 caacggaggt gagacctgat ggctgaacgc aaacagaagg taagcattcc ccttacggtt
     9841 ctttccgtgc tcatcgcggt aatcgtcgtg tttcccatcg tctggatggt gctcagctcc
     9901 ttcaagcctt cgggcgagct gttcagctat ccgctgcacc tgttttccca ggaccccacg
     9961 gtcgagcatt acgcctctgt gctggcgggc ggcttcatgg gttatgtaaa gaacagcctg
    10021 ttcctggctg tcgtaggcac gctcatcacg ctggtcatca gctccatgtg cggttacgcg
    10081 ctggccatct accgctttga gatcaagtat gtcaacctgg tgttcggcgt tttcctgctg
    10141 ggcacgctca tcccgggcga gacgctcacc gtgccccagt tctccgtcat cagcgcgctg
    10201 ggcctgtaca acaacatctg gggcgtcatc cttcccgtcg tcaccacgac caccggtatc
    10261 ttcatgtacc gtcagcacta tatgtccatc ccgctctctt tcgtggaggc cgcgcgcatc
    10321 gacggtgcga acgagttcgt cattctgtac cgcatcgtcn nnnnnnnnna ttacattctg
    10381 ccgctgctgg tgctgtctga tcaaaagaaa tacaccatcc agatcgccat caaaaacttt
    10441 atcggcaatc tgggcgttga ctggagctct atcctggcag cctccgtgct gtccatccta
    10501 cccgtcatcg tgatttttat cttcctgcaa aaatatatca ttggcggcat ctccgcctcc
    10561 ggcgtaaagg gctgacctgt ttttacagcc cccgcggggc cgccggagcg cgcagcacgg
    10621 ttttttatta tagaaattgt aaagttatgg cc
//
LOCUS       DAWBSB010000063        10594 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1442_length_10594_cov_3.112250, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000063 DAWBSB010000000
VERSION     DAWBSB010000063.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 10594)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10594)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10594
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1442_length_10594_cov_3.112250"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2125 a         3324 c         3055 g         2090 t
ORIGIN      
        1 acatttcgca gctgcgcgcc cgccagtgtc aaatggcctc cgccgcccag cttttccata
       61 atgacctgca cgttcacatc gcccatactg cgggcggaca ccgacacctg cgcgccgtta
      121 tccaccgcca caaagctggc ctccacaccg ttgatggtga gcaggtcgtt ggcggcctgg
      181 ggcactacga cgctgagctc cgcgggcacg ctgtcggaaa acacgacggc acagcccata
      241 tagatttccg cgtccgtgac aagctgcgcc ttgtaggcat aactttcaaa cgacgaattg
      301 aacagtttct tcactgcctg cgtctgcgcg cccatgcgcc gtaagtaagc ggctgcctcg
      361 aacgtgcgca cgcccgtgtg cagcgagaaa ttgcgcgtgt ccagcatgat gcctgccagc
      421 agcgcctgcg cttccagcgg cgtaaggcgg ttctccttgg tcatgtcgat gtactggagc
      481 agctcgctca ccagctcgga tgcgctggaa gcataaggtt cgtgatagaa tactacactg
      541 tcgtcaatgt ggcccacaca ttttcggtgg tggtcgataa cgactacggt ttttgccgcc
      601 ctgtacagtt ccggactttc caaaatggcc ttcagatgcg tatccacgat caccagcagc
      661 gtgtttttat caatgcattt ttccgcttcg tccggttcga taaaatcatc cccgcagcca
      721 gcgcggcgga attcctcaat gaggtttgcc gcgaggctct ggcgctcgtt gaccaccacg
      781 gcggcgggtt tgccgcacag cttcgcaaag cgcagcatac cgacggcgga acccaaagcg
      841 tccaaatcgg acgcgcggtg gcccatgatc agcacgcttt cgctctgtgc catcagatcc
      901 ttgatggcgg aggcgatgat gcggcttttc accttgctgc gcttttccac actgcgggac
      961 acaccgccgt agaactcaaa gccctcggca cttttgacgg cggcctggtc gccgccgcgt
     1021 cccagcgcca tatccagcgc ctgcacagcc atggcctcgc cctcgcgcag tgtggcggcc
     1081 agccgcccta cgccgatgga gagcgtcacg gtgcccgcct cttcgccgat ctcgcgcact
     1141 ttatccagga tggaaaagcg gtttttaaca atctcctgca tatggcgctc ttccaccaca
     1201 gcgatatacc gcgaagcgct gatgcgccgc agaaagcctg tggtggcccc gatgaactgt
     1261 tccagcgcca gatcgatctc acccgctacg cgggcgcggt cgctctcttt catttccttg
     1321 aggatctcgt cgtaggtatc cacggcaatg agcatcaccg cggggcgtgt ggcaagatac
     1381 tctcccgcct gccgcttgag cgtggtattg ttgacaaaat aggccacgaa caggccatcg
     1441 cccgcctcca cgccgctgcc gtacacggaa taacgctggc cgccgaactc gatgttctgg
     1501 cccgtctttt ctttcgccag cgccggcatg aacgccggga ccaccttcga catgggcagc
     1561 aggcacacgt ccctgccgcc cattacagcg tcacggaacg catcgttgta ccacgccacg
     1621 ctgccgcctg tgaggataac gaccgggacg ctcatggcgg caaaacttcc ctgcgccgcg
     1681 ctggatgcat ccgaagcgcc gtgcagcaga cgcgccagcc tgtgccggaa ccagcggatg
     1741 ttcagcccga ccacgatgcc cacaacgagc agcacacccg cgacaaaaac aaggtaagtg
     1801 ggcttcacca gcgccagcgc aacgcacagc gcgatgcagg ccgcggacag gatgatgacg
     1861 gtcgcgtcaa gcgccgcaaa tcgttttttc atgccgtgcc tcctcttttc tcaggcgccg
     1921 ccgctgcggc gcgcctcata cctccatcaa aatgggcaga atcatggggc tgcgctttgt
     1981 cttgcggtac aaaaaggcgg acaccgtctc gcggatacgc gttttgacgc tgccgaaatc
     2041 gcgcttgtcc gccgcactca tgcggcccag cacatcgcgc acctggtcgc gggcgtcgtc
     2101 cagcagcttt tcgctctcac gcacatagac gaacccgcgg gacacaaggt ccggtccggc
     2161 caatacttcg cccgtgcggg cgtcaatggt agccacaacg ataacaaggc cgtcctcaga
     2221 gaggtgacgg cggtcccgca ggaccacgtt gcctacgtcg cccacgccca ggccgtccac
     2281 catcaccgca ccggcgggca ccggctcgcc cacggtaagg ccgtcggcgg agagtaccac
     2341 acagtcgccg ttctcaccga tataaatatt cttttccgga atgcctacgg cagcagccgt
     2401 ctgggcgtgc tttttcagat gcttgtattc gccgtgcaca ggcaggaaga actggggctt
     2461 tgccagcgtg agcatcagct tctgctcctc ttggcaggcg tggcccgaaa catgcacgtc
     2521 atacatggat tcgtagatga cctcggcccc cagcttcaaa aggccgttga cgaccttcgt
     2581 caccattttc tcgttgcccg gaatagggtt tgccgagatg atgataaaat cgccctcgcc
     2641 cacacgcacg ttgcggtgac tggcagcagc catgcgggac agcgcgctga gcggttcccc
     2701 ctggctgccc gtggtgatga gcacgatctt ctccggcgga tatttgttga tgtcctcaat
     2761 atcgatgaga acagtctccg gagcgtgcag ataacccagc tccagcgcca tctgagtgtt
     2821 gttcaccatg ctgcggcccg acacggccac ctttcggcct gcctttacgg caaggtcgat
     2881 gagctgctgc acgcggtaca ggttcgaggc gaacgtggcg atgatgatgc gctttttgcc
     2941 cgcgcggctg aacagggcct ccatgctggc gcccaccttc tgttcggtgg ccgtgaagcc
     3001 cggacgctcg gcgttggtgg aatccgacaa cagggccaaa acgccgtgct cgccgtattc
     3061 ggcaatggtg acaagatccg tcgggccgcc ggaaataggc gtgtagtcga ttttaaaatc
     3121 gcccgtctgg atgatggttc ccgcagggca tgtgatggcc agggccaccg cgtcggggat
     3181 ggagtggttc acgtggatgg gctccacggt gaaacagccc agcttgaact tctggtgcgg
     3241 agcgatctcc accattttcg tgctgcgcac cagcccgtgt tcttccagct tgttggtgat
     3301 gagcccgatg gtaaggcgcg tggcataaat gggcagattg acctctttga gcaggtaggg
     3361 caggccgccg atgtggtctt catggccgtg cgtgataaca acgcccttga tgcggtcctt
     3421 attctggaca acataggtga aatccggaat gacaaggtcc accccgaaca tgtcgctgtc
     3481 cgggaacacg atgccgcagt ccacgaggat catgtcgccc tgacactcgt acagagtgat
     3541 gttcttgcca acctcgccca gaccgcccaa cggcatgatc ttgaccggga cagccggggt
     3601 gccggcgctt gcagcggcgg gtctgcggcg acgcgggtgg tacggcctgc gcgcaggctt
     3661 cggcgcagcc gcagggaccg cgcccgtctg ggaagctgcc ggcttcgccg ccggagccgc
     3721 agtgttctgg cgcgtgtcgg atacgccggg ggttgccgcc ttgggcgctc tgggcgcgcg
     3781 cggccgggat gctttttgat tattttccat tcgttcctcc atttcccgcg cgctacaaag
     3841 aagccctgcc gttgttcacg ggcaggccgc ttttcgccgc gcatctgcgg gaattctttt
     3901 ttattttcgt tttttatcag cgggataccc cgcaaatatt taaaaataaa acggcccggc
     3961 cggacaatgg gatgccccat gcataaaacg gcccgccgga cggctttata aaacgtcgcc
     4021 tgaatgcggt tattgttatt ctaaataata ccgtttttgg gtagtattgt caagttttgc
     4081 catcgtgctt tgtgagtatt ggataaaaaa taccgcatta ttcgcacaga gtgcttcgtt
     4141 tttatcgcca gcgttccggc acgggctgct ctgctgccac ttgcatggcc gcctggtttt
     4201 ctctataatg atagagtctg ttatgagttt atgagaatac ggagcgtttt tatgaaaaag
     4261 atcacgatat acaccgacgg cgcgtgcagc ggtaacccgg ggccgggcgg ctggggcgca
     4321 attctggaat acaaaggcgt gcgcaaggag atctccggcg gcgaggcgga gaccaccaat
     4381 aaccgcatgg agctgatggg tatcatccgc gcgctggaac agctcaaaga gccttgcgac
     4441 atcaccttct gcagtgattc caaatatgtg atggacggcc tttccaaagg ctgggccaag
     4501 ggctggcgcg cccgcggctg ggtaaaaagc gacaaaaagc ccgcgctgaa cagcgacctg
     4561 tgggcgcacc tgctggacct gtgcgaaggc cacacctttc attacaattg ggtaaagggc
     4621 cacgccgggc acccggaaaa tgaacggtgc gacgagcttg ccgtgcaggc ggcggcccag
     4681 tacaagtaat atcggcaaaa aagctgccgg aatatataat tttttccaat tgcctgttgc
     4741 aaaccacacc aaaatagtat atattataga aaggccggac acactgctcc tgaccgtctg
     4801 cgggcagcgg ccattcaaaa acaaggcaag gtgaaaactt tgagcaatat catctatgtg
     4861 gacaacgccg ccaccacgcc tgtgtcgaag cctgttttcg acgccatggc gccctatttc
     4921 tgcgaacaat acggcaaccc gtccagcatc ttttattccg tcggcgcagc ggcgggcgac
     4981 gcactggccg ccgcgcgcga aaaggtggcc cgcgcgctgg gctgcgatac cggcgaggta
     5041 tttttcacga gctgcggctc cgaggccgac aactgggcca tcaagggcac ggcgcacaaa
     5101 tttgcaaagc agggcaaaaa gcacctgatc acaagctgta tcgagcatca cgccgtgctg
     5161 cactccatgc aggcgctgga acgcgaaggc tttgaggtga cttacctgcc cgtagacgcg
     5221 gagggctttg tctcccccgc cgatgtggaa gccgccatcc ggccggacac agcgcttgtg
     5281 accatcatgt ttgcaaacaa cgagatcggc accatcgagc ccatcgagga aatcggcgca
     5341 gtatgccgca agcacggcgt gtggttccac accgacgcgg tgcaggctgt gggtgctgtg
     5401 cccattgact gccatgcgct gcctgtcgat atgctgagcc tttcagccca caagttcaac
     5461 ggaccgaagg gcgtgggggc gctgtatatc cgccgcggcg tgatgccgct gaattttctg
     5521 gacggcggcg cgcaggagcg cggacgccgc gcgggtactg aaaacctcgc gggcatcgcc
     5581 ggcttggcca cagcgctgga agccgcagtg aacagccttg acgaaaagcg cgcacggctt
     5641 gcccggatgc gcgactatgt gctggagcat atccagaaga tgccccaggt aaaagtgaac
     5701 ggcccattgg acggcagcaa acgcctgccc aacaatgtga acgccagctt caccgcggcc
     5761 gagggcgaaa gcctgctgct catgctggac atgcgcggcg tctgtgcctc ctccggctcg
     5821 gcatgcgcct ccggcagctt ggaccccagc cacgtgctgc tttccatcgg cctgccccac
     5881 gagatcgctc attgcagcct gcgcatcagc ttcggcgtgc agaacacgat ggacgacgcg
     5941 aaggccgtgt gcgacgcgct ggatgaggtc gtgcccgccg tgcgccagcg cagcccgctg
     6001 tggcgcgcat gattttaaga tgtgaatttc cggaaagaag gacaaaggat tatgtatagt
     6061 gcaaaagtga tggaccattt tgcgaacccg cgcaatgtgg gcgagatcga gaacgcggac
     6121 ggcgtgggag aagtcggcaa tcccaagtgc ggcgacatca tgaagatgtt tttgaagatc
     6181 gacaacaacg tcatcaccga cgtaaaattc aagacgttcg gctgcggcgc agccatcgcc
     6241 acttccagca tcgccaccga gatgatcaaa ggcaagacca tcgacgaggc gctgaagctg
     6301 acaaacaagg ccgttgtgga agcgctggac ggcctgcccc ccgtgaagct gcattgcagt
     6361 gtgctggccg agcaggcggt gaaggccgct ctggccgatt actaccgccg ccagggcgtg
     6421 gaccccgagc cgattgtagg caagctggag gaggattgcc acgcctgtgc ctgtgaggca
     6481 tagttcccct ttccccgccg taaaaacgaa tggaccgccg cgcggccggg cagccggttt
     6541 ttttcgatat ctttgaaaaa gcataaaagg agttttcctc atgacggatg aaatctgctg
     6601 cggcagcagc ttcggcacct ttgaagagca ggacaaggtc cttgtcgcgc tttccggcgg
     6661 cgtggattcc tcggtatgca tccagatcct gcgcgaccaa ggctttgacg tgcaggcggt
     6721 ggtcatccgc ttttctcctg ctcacgatgc ggctgtgcgg gccgctcaga cagtggcgcg
     6781 ccagctgggc gtaccgctta tcgaggagga ctgtacagaa gagttcgaac agcaggtggt
     6841 ggaaccgttc tgcgcgcaat actgcgccgg gcgcacgccc agtccctgcg tgctgtgcaa
     6901 tccccgcgtc aaatttgcag cgctggcccg tgtggccgac cgtctgggca tccgctatat
     6961 cgcaacaggg cattacgccc gcgtaacgga ggagaacggc ctttactatg tacgcgccgc
     7021 cgtcagcccg gagcgcgacc agagttacat gctgtatggg ctgccccaga atattctggc
     7081 ccgcctgtgc ctgcctgtcg gcgagtttga aaagtctgat atccgcgaga tggccgcgtc
     7141 cgcgcagctt gccagcgcgg atgcgccgga cagccaggag atctgcttca ttccggacgg
     7201 cgactatgcc gcctatatcg ccgcacgcgg cctcgttcct ctgcagggcc gcttcatcgg
     7261 tcccgacggc gaagacctcg gcccccataa gggcgtgtgg cattacaccg tagggcagcg
     7321 caagggcctg aatatcgcct acggcactcc tctgttcgtg cggcgcatcc gcccgaacgg
     7381 cgacgtggag cttgcccgcg gcggcgatga atttttttcc ggcatagtac tgacagacgc
     7441 cgcacgcgcg gacggcagac ccatcctggc tggggagcgc tacggcgtaa aggtgcgcag
     7501 ccgcgcagcg gcggcgtcct gcactgtgga agccgcggaa aacgacagcg tgacgctgcg
     7561 ctttgacgag ccgcagcgtg ctcccgcccc cggccaggcc gccgtactgt acgacggaga
     7621 actggtgcgc ggcggcggta ccatctgtga aatgctgtaa atttttcacc agtgtccccc
     7681 gtgtttgctt tgccccgtaa taaaaaacgc ccgccggcac gcgggacacg catgaatgaa
     7741 atcaccccgg cctataatag gccgaggtga ttttttatgt tcatttatct tggagacaat
     7801 gactctgtgt ttgaagcagc cctgcgtgag gattcttccg tgcgggccta ttatgagagc
     7861 ctgccgggca tgctgcggga aaaggtgcgt gcagctgggc tgtatagcgc acaggagatc
     7921 ggcgcatata tcgatatgct gatagccggc ggaaactgac gcgcacgcat cacgcgccgc
     7981 cgtatacgcg gtagcatccc ttgcccgccg cctttgcggc atacagcgcg gaatctgctc
     8041 ttcgcagcag ttcttcataa gcgcaggctc ccggcttcga caaaacaaca ccgatgctgc
     8101 cggagacgtc cacgccgcat tgcacgctta cggagcggca catttcctgc gcgatcttcc
     8161 gcgcgcgtcc cactgcctgt tcgccgcccg cgcccggcat catcatcaaa aattcatccc
     8221 cgccgacgcg gcccaccaca tcatcccggc gaaacagccc ggacagattt tcgcccaaac
     8281 agcgcagcac cgcgtctccc tttggatggc cgtaacggtc gttcgtctgc ttaaagttgt
     8341 ccacatccag caaaagcagt gcaaatggtt tttcaccggg ggtgttctcc aaaagcgtac
     8401 ggcagcgctg ttctgtcgtg cgcttgttca aaacgcccgt caggctgtcc gtctgcgtca
     8461 ggcgcagcag gcgtgcccga tcctcgtgct gaggcgtgac atccatgata cagcccacag
     8521 cactctctct tgcgccgctg ctgccgcgca ctgccaccag tgaaacgcgc acccatatcc
     8581 cgccgccggg cagttcaatc cggcgttccg caccgcacac atcatcgccg tcctcctcca
     8641 gcgcctgtgc gatacaggcc gcataagatg cgaacggctg ggaaaagcgc ccggcatgga
     8701 cctcggcgag gaaagccggc gcgcgctccg gcagttccaa gcccgattct gaagcgccgg
     8761 caaaggtaag catgtccgta ccgtattcat attcaaacag gacctgcccc agcacacgcg
     8821 ccaagcgctt gtagtgatgc acgaaccgtg tttgaagcgc atgtctgttg gtaaaatcca
     8881 aaaacacgac ttgaataacg gacccctccg ggccggacag catctgaagt gtcccgaata
     8941 cccagcacac cgagccatcg cggcgcacca gccggcactc gaagctgcgc gcgtcgcccg
     9001 ggcggcgcag ccgggacaga ttacccaacg caccgcgcct gtcgtccgga tgaacgcacg
     9061 agaggacatt cgggtccggc tggctccaga agtcctccag gctttcgtat cccagtatcc
     9121 gtgccccggc cagattcgta tgaatggcat ggtaccgtcc gtccgcctcg gcccggaact
     9181 gtgcgacggc acacgggatg ctgctgagca tccccgcata aaaatcccgc tcctcagaaa
     9241 gttgcttgat ctcgcgtgac tttgcggccg ttcccgtctg ctcccgggtg atatcgtaga
     9301 aaaagcacag cagatagcgg ccccattccg gactgcccgc atatgttgcc gtcatgcgta
     9361 cccatatcac gctgccatct gcccggcgaa cagggagctt ggtgtcgcac cggcccgccg
     9421 taccgaactg acggcgcatt tccgcataaa ggcgggcctt ctccggctct gtggcaatat
     9481 gggcaaaaaa tgctttctcc atttccgcca tctgtccgcg cggatacccg cacagcgaga
     9541 acagctgctc gtttgcaaac agcagctgcc tgctggaagg gtccactccg gccacggcac
     9601 agggcactgc gtcaaacagc gaatgagtga tggaacgcac ggcgctgtcc gtcagggcgg
     9661 acgcttcaca gtagtcaggc agccgctgcg cgccatctgc gccgccccac ggcagcgcac
     9721 aatagccgcc cgcctgctgt acgaacgctt cgaacgcttc cgcaggcatc ggctgtgcaa
     9781 aatagtagcc ctgtgccagc gtacagccga acgtattcag ccgttccacc tgcccggccg
     9841 tctccagccc ttcgcagagc accgtcatgt acagctcatc cgccatgcgg atgatattgc
     9901 gcaggattac atcgctttta tagccttccg ctgcagcgcc cgaaacaaag ctgccgtcca
     9961 gcttcaggat atcaaactcc agttccttga gcaggttgag cgcggaatag cctgtgccga
    10021 aatcgtcaat ggataccttg cagcccagtg cgcgcaggcc gcggattaca gccgccgcct
    10081 cttccatgga ttccgccagc atggtctctg tcagctcgaa ttccattaag gacggatgca
    10141 cgccataccg tatcagcagc tgttttacct tttggacgaa atccgtgtcc ttcagatggg
    10201 cgcgggacac atttacagac acggggacaa cgatgctgtc tcccgcctgc ctcccgcaca
    10261 gataacggca tacctgctcg taaatcatga aatccagctg ttccacattt cccgtgcgct
    10321 ccaaaagcgg cacaaactcc gctgggcccg ctacagtgtc gtccggtttt ttccagcggg
    10381 ccagagcctc cgcccccgct acacgccccg tacgcaggtt taccttgggc tgcaaataaa
    10441 aagtgaactg ctcccgttcc agcgccgcct gcaggtccgc ctgcaaacgg atggcgcgcg
    10501 ccatctcgtc tttgagtgct tcatcaaaca cagcgcaggc agtgcgatac ttcttttttg
    10561 cctccttgtg cgcgatattc gcaccgtcaa tggc
//
LOCUS       DAWBSB010000064        10589 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1445_length_10589_cov_2.791912, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000064 DAWBSB010000000
VERSION     DAWBSB010000064.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 10589)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10589)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10589
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1445_length_10589_cov_2.791912"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    841..850
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2208..2217
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3902..3911
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7407..7416
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9842..9851
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2405 a         2772 c         3334 g         2028 t
ORIGIN      
        1 ggacatcact ctgttgggcc agaatgtgaa ctcctacggc aagggcctgc cggaagacat
       61 cacctttgcc gcgctgctgc gcatgctgga cgctgttccc ggcgattacc gcatccggtt
      121 tatgacgagc catcccaagg acgctacgcg cgagctcatc gacaccattg caaacagcgc
      181 gcacatcagc cgccatttgc atttgccggt gcaatccggc tcgaacagca ttttggcgca
      241 gatgaaccgc aaatacacag tggaggacta cctctcgctt atcgcttacg ccaaacgccg
      301 catgccggag atgacgtatt ccagcgatat catcgtcggc tttcccggcg agacggaaga
      361 ggacttctgc gccacagagg cgctcatccg cgaggtcgag tacatgcagc tgtttacgtt
      421 catctactcc aaacgcacgg gtacggcagc ggcaagcatg ccggacccca caccccacaa
      481 ggaaaaaacg cagcgcattg cccgggtgct tgccattcag gaacaggtgg tggcaaaact
      541 gggcggcgca atggtgggca agaccgtccg cgccctggtg gaaggcccgg gcagggagga
      601 gggaactctt accgcccgtc tggacaacaa tatgactgtg gattttgcag gcggcgcgcc
      661 tgtgggcgag ttcgccgatg tccgcgtgac aggcgtgcgc ggcgcgatgc tgaaaggcga
      721 gcttgcatga acccgcccgc cgcagggcaa aataatgcga ccccatcgga aatgcttcaa
      781 aaataaaggg aataaaagga gaattttatc atggcgaaag acgcgatcac gatgtttaag
      841 nnnnnnnnnn atgtttaagg aggctgccgc acagctgcag cgggaagagg cttatctggc
      901 gctggagggc acccgcaaaa aaaatgacga ggatgaggcg cttcagcagc tgatcggaga
      961 ctttaatctt gcccgcatcg acctgaacag cgagctttcc aaaagcgcgg acaaggacca
     1021 ggataaggtc aacgaactga atgtgaaggt gaaccagctg tacaacgaca tcatggcaaa
     1081 ccagagcatg gccgcctaca acgaggcaaa ggctgaactg gagtctgtga tgaattatgt
     1141 gaacgccatc atcaacacgg ctgtcaacgg cggcgacccg atgacggtgg aggagccgca
     1201 gcagggcgga tgctcgggca gctgcgcttc ttgttcgggc tgccactgat ggcggccccg
     1261 cgctccgcgc gttttgaatc ttaataggac ggtgcagaat atggcagcgc tttcccccat
     1321 gatgcagcag tatttcgaca tcaaaaaaca gcatcccgac aagatattgt ttttccgtct
     1381 tggggatttt tacgagatgt tttacgacga cgccattctt gtgtcaaagg agctggagct
     1441 caccctgacg ggccgggact gcggccagga ggagcgcgcg cccatgtgcg gtgtgccgtt
     1501 ccattcctat gaaaactatg tggcaaggct cattgccaaa ggttataagg tcgccatctg
     1561 tgaacagatg gaggaccccg ctctggcgaa aggcctcgtc aagcgggata tcatccgtgt
     1621 agtgacaccc ggcactgtga tcgaaagcag tatgctgcag gatgataaaa acaactacat
     1681 cgccagcatt tatatcacgg agaaaaaagc gggcgtatgc tttgcagacg tctccacagg
     1741 cacggctcac gccacggtgc tcacgggcga aaagctgggg ccggagatca tctccgaatt
     1801 gtgccgctat tctcccagcg aggtgctgtt caacgcagct attctggact acaaggaagt
     1861 aacggcgtat atcaagcagc agcttgcctg cagtgtggag ctgctggatg aagcggcttt
     1921 cgcgcccgac gcctgcatgg aagagatgcg cgcccagttt ggggatgccc cggaaaaaac
     1981 ggccggcctt gcgccggaaa gtccggcgtt cacggccctg gccgtgctgc tgggatactt
     2041 aaaggaaaca cagaaaaagg gcgtggagcg gttaaagacc gtacacaatt acgccgaagc
     2101 gcagtatatg cagctttcgc ccgtcacgcg cgccaacctg gagctgacgg aaacgatgcg
     2161 cgggcgcgag aaaaaaggta cgctgctgtg ggtgctggat aaaacgcnnn nnnnnnngga
     2221 aagcggctga tgcgcgcgtg gatcgagcag cctctggtga acgtggcggc catcaatgcg
     2281 cgtctggacg gcgtggagga gctggtgggc gattccgtag cccgcgccga tatcgcggcc
     2341 gcgctctcaa agatatttga catcgagcgc ctgatgacgc gcacggtgta cggttcggcc
     2401 tctccgcggg aaatctacgc gcttgccgcc acctgtgagc agctgccccg cttgaaggcg
     2461 ctggcccggc ccttcggcag cgccgagatc accgcgctgc tggctgatat cgacgaattg
     2521 tccgatatca aggagctgat cttcgcagcg gtcgacgaga atgcgcctgc catgctcaag
     2581 gacggaggcg tcattcaccc gggctacaac accgaggtgg acgagctgcg ggacatcgtc
     2641 cacggcggca aaggttacct tgccacgctg gaggcaaagc tcaaggagga aacgggcatc
     2701 cgcacgctga agatcggcta caaccgggtg ttcggttatt acatcgaggt gagccgttcc
     2761 ttttcgaacc aggtgcctgc aaattttgtc cgcaagcaga cgctggccaa cgcggagcgg
     2821 tacatcaccg aggacctgaa ggtactggag aacaagatcc tgggcgcaaa cgagcgtctg
     2881 gctgtcctgg aacgccagct ctttgacgat ctgctgcata aaatttcggc cgaactgccg
     2941 cgtatccaga aaaccgcgtc cggcgtggcc cgtctggacg tcctgacggc gctggcggag
     3001 gtggctgtga aaaacggcta tacaaagccg gttgtggatg aaggcgacga gctcatcatc
     3061 gaggagggcc gccatcctgt catcgagcag atgctcaagg gtgcgctgtt cgtgcccaac
     3121 gacactgtgc tggactgcgg cgataaccgt atgctcatca tcaccggccc gaatatggcc
     3181 ggcaaatcca cctatatgcg ccagacggcg ctcattgcgc tgatggcgca gatcggcagc
     3241 ttcgtgccgg cccgggcgtg ccgcatgggt gctgtggatg ctattttcac ccgcgtgggc
     3301 gcttcagacg atttggctgc ggggcaatcc acctttatgg tggagatgac cgaggtggcc
     3361 gaaatcctcc agtacgcaac ggcaaaaagc cttgtgattt tagatgaaat cggccggggg
     3421 acttccacgt tcgacggcat gagcatcgcc cgcgccgtag tggaacacat tgcggacaag
     3481 gatggcggca tgggctgcaa aacgctgttt gccacccatt atcacgagct gacggatttg
     3541 gaaaacgcca ttgacggcgt gaagaattat aacatcgctg taaaaaagcg cggcgacgac
     3601 attacgtttc tgcggcgcat cgtgcgcggc ccggcggatg acagctacgg cattgaggtt
     3661 gcaaagctgg cgggtctgcc cggcgaggtg acaaagcgcg cgaaggaagt gctcaaggtg
     3721 ctggaggcca ccgcgcccaa cgccgataag gtgacgcagc tggacttcgg caattttgaa
     3781 aaattcgcgg cagcgcccgt gcccagcgag ctggtggaca agctgacagc ggtggatgtg
     3841 gaaacgctca cgccgctgga agcgttgaat tttttgtatg aactgaaaca gacactggta
     3901 annnnnnnnn ngcgcccggc gcgcgggaaa gggcggtgct gcgcattggc agttattcat
     3961 gtgttggaca agcataccgc ggaactcatc gcggcaggcg aggtggtgga gcgccccgcc
     4021 tcggttgtaa aggagctgac ggagaacgcc attgacgcgg gcgccaccgc agtttccgtt
     4081 tccgttgaac gcggcggcat cgcaatgatc caaatcgcgg acaatggctg cggcatcgag
     4141 gccgagtaca tcccgacggc gtttatccgc cacgcaacga gcaagatcga gaaagaggaa
     4201 gacctggaga gcattcatac cctgggcttc cgcggcgaag cgttggcctc cgtggccagt
     4261 gtgtcccgca ttgagcttct cacaaagaca gatgcggacg agtatgcata tctgtaccgc
     4321 atcagcgggg gagaagaaca gggcatcgag ccggccgcac gtcctgtcgg aacaacgatc
     4381 actgtgcggg acctgtttta caacacgccc gcacgtatga agtttttgaa aaaggatacc
     4441 agtgaaggca actatgtggc agatgttgtc acacagcttg cgctctccca cccggaggtt
     4501 tcgttcaagt tcgtgcggga cggaaaaccg cagtttcaga cgcccggcga cggaaaactg
     4561 ctgggggcgg cgtatgccgt gctttcccgt gattttgcaa aggaccttgt gcctgtggag
     4621 cacacggcgg gcagctacac ggtgcgcggc cttgttacgc cgccgaaaaa cgcccgtgca
     4681 agccgcgcca tgcagtattt ttttatcaat ggacgctttg tcaagaaccg cacgatgatg
     4741 gcggcgctgg aggctgctta taaaggcgtg atgatgcagg gaaaattccc gggctgtgtg
     4801 ctttccatcg agatgccgcc gcagctggtg gatgtgaacg tccacccggc caaaacagag
     4861 gtgcgttttg cccgggaaaa agatgtgttc gatgcggtat atcaaagcgt taaatgcgta
     4921 ctcatcacgc cgcagagcac ggaaaaggtg ttttctttcg gcggtgcgga aagggcgttc
     4981 ccggcggatg aaaaggcgcc ggatgcagct gcgaagaagg agccgtccga gagcgccccg
     5041 gccgaagagc ccgcgcgcgg cccgggcgca ttgcgcccgg cgtcccgtgc cgttccggac
     5101 gcaccggtga ggcccgtccc ggcacgtccg gagccgtccg ccgtaccgct gcccggccag
     5161 gtgaaagcgt acagtgaaat gctcactgct ctctcaagtg aatctaccgt tccgtaccgt
     5221 tcggcaaagg cgccggatat atgggctgat gaggacgcgg cggaggatgt cggctttatg
     5281 ccgcacactg tctgccggcg gcaggaggga acgccgggcg tgcagaccct gccggaggac
     5341 cgggccctga acagctttgc ggcgggagaa acagccggga cggaaaatac cgccgcgccg
     5401 cagatggagg ctgaaacgcc ccatcagact gcttttgcgc ctgtggaaga agaacagcgg
     5461 cgcgatccgc ttcgttttgt gggcgaggtg tttgacacgt acattgtcac ccaacgggga
     5521 aacgacatgt gccttttgga taagcacgcc gcccacgagc gcattatcta tgaatctttg
     5581 gcggcatcgt atgggaaggt gagcagccag atgctgctgg tgcccgtaac ggtgcagctt
     5641 tccgcggaag aaaaagatgc actggtgcaa agtgaaatgc ttttacagga cagtggtctg
     5701 gaagtggagg acttcggcgg caattctgtc gtggtgcgcg ccgtcccggc ggacgtcgtg
     5761 cccgaggatg tggaggacct tgtggtggag ctggccagca ggctggtctc caacccgcgg
     5821 tataccacca gcgaaaaaac agaatgggtg ctgcactcca tcgcgtgccg cgcggccatc
     5881 aaggcgggag ataaaacgca cgctgcacag ctgctgcgct tggcggagga tgtgctggac
     5941 ggcagagtgc ctccgttctg tccccacgga cggcccatcg tgctgaagat caccaaaaag
     6001 gagctggaaa agcagtttgg cagacaaggc tgatatcatc gtgatctgcg gtcccacggc
     6061 tacggggaaa acagcgctct ctgttgcgct ggcaaagctg ctggacggcg aggtcgtttc
     6121 ggcggattcg atgcagatct ataaagggct tcgtgtggga actgcacagg cgacgccgga
     6181 ggagatgaaa ggtgtgccgc accatctggt gggatttctt ccgccggaag aacggttctc
     6241 tgttgcggac tatgtcaggc acgcaggcga gtgtatcgcg cagatcacgg cacgcggacg
     6301 cgttccaatc gtggccggcg gcaccggatt gtatatttcc agccttgtaa acgggataga
     6361 ctttacagtg gaaaagacag acccagccgt gcgggcacgg ctggaaaccg agctgatgga
     6421 gcagggcatc ggcccgcttt atgcgcggct gcagcaggtg gacccggagt gtgcggcgaa
     6481 gctgcacccc aacaaccatg ggcgtgtgct gcgtgcgctg gagctgtatg agcaaacggg
     6541 cgagacgatg tcctgccggc tcgcgcgctc ccgtccggcg cagcagccgt accgcgcttt
     6601 gctgataggg ctgaatatgc cggagcgtgc acagctttat gcacgcattg atgcgcgagt
     6661 agacgcgatg cttgcacatg ggctgctgga agaggcgcgc ctggtgtatg ccaacagaga
     6721 tgcctaccat accgcggcgc aggccattgg ctacaaagaa ttttttcctt attttgaagg
     6781 aaaggaaccg ttggaagtat gcacggcggc gctcaagcag gcgtcccgta attacgccaa
     6841 acggcagctt acctggttcc ggcggatgga cggcctggtt tggctgcccg caggggagcc
     6901 ggaagcggcg gaggcggccg cccggctttg ggcggggcat gtgaagaaaa attgcggcga
     6961 tcaaatataa ggaataacgg atatatggaa aactggccgg taagaaaaaa gaaaaaacgg
     7021 tacagaggac tgattttggc tctgctgatg ctcgtgcccg ccggatatat cggcgttcag
     7081 atggtacagg tgctgcgtac gaattatcaa acgcagaccg cggtggcata ttcaatgtcc
     7141 gacgtcgtcc gctgcgacgg gatgctcgcc atggatgaga cggtcattcc ttatgaggga
     7201 gcgggcgtcc tgggctatca ggtgaacaac ggcgagcgtg tctcggcagg cacgcaggta
     7261 gcgcgattgt tcgccgacga agcgagcgca cagaaccgga cgctctccga aaagctgacg
     7321 gaggagcttt cggtgctgga aaagtcccag gcgggcgcgg ctgccgacgt tgaggcgctg
     7381 atgaatcaga accagcaggg tatctannnn nnnnnnggat tatcggatgc gcgggcttcc
     7441 atccagcttg cgcagaataa gatgcagctc acgatggggg tcgtatcgga tttcagcgcc
     7501 cgcattgcgg acctgacggc gcggcgcgac gcggcggatg ccgccagcgt atatacaccg
     7561 ataacagctc cggcggccgg atattttgtc tccgcgcagg acagcgagaa gcagatgtat
     7621 acgcctgagg cgctggcggc catgtctccg gcagaattga aggatgcgct ggcacagcct
     7681 tcccaaacga acgacgcaaa cgtagcgggc aagctgattt tggactaccg ctggcgttat
     7741 tatggccttg tgacgcagaa acaggcggaa aagttcgtgg agggaacgcg cgtcgagatc
     7801 tcgttcccaa acgtatcggc cgaatcggtg cccgccaccg tggtgaatgt tacggtggat
     7861 gaggagaacg gtactgccaa ggtggaattg ctttgtgatt acatcaacga gacagttgtg
     7921 acgctggagc atgaaaaagc agatataacg ttcgccacct atgagggaat ccgtatcgac
     7981 cgacaggcac tgcacatcgt tgaagggcaa aactgcgtgt ttgtcaaata cggaaacctg
     8041 gtgtaccaga aaaatattac gatcctgttt gaaaacgagg attacattct ggtgccgtcg
     8101 aaaacaacga cgggcgaaaa tgaggtaaaa ctgttcgatg agattgttgt gcgcggtaca
     8161 gatctgtacg acggaaaaat attgtagctt ttttgtgtgc ctttttacac aaatttgcaa
     8221 ctgtttattg acatccgcca caaaaacaag gataataacg atgatagtat ccaatttgtt
     8281 gtgccggtgt ggatacaaaa ccgttacaat cgtggcgcgg cggcgcatgc gtctgcgcgc
     8341 ggccagcttg ccataaaaag gagatttcag agatgagtat gttcgataag atcaaaggta
     8401 tgatgggcgt gcccgacgac gacgattatg atgacgacga tatggatttc gtgaatgaga
     8461 atacaggcta tgcggcgcag gacgattatg tccccgaaga acaggatgtt acgcccaaac
     8521 gcaataaggt cgtgaatatc aactccacca tgcagctgca ggtagtgctt gtgaagcctg
     8581 agcgttttga tgatgccagc gccatcgcgg accatctgaa cgccaagcgc accgtggttt
     8641 tgaacctgga atccacaaat aaggaggtct cccgccgtct ggtggatttt ctctccggcg
     8701 tggcgtatgc caacaacggc cagatcaagc gcgttgccaa cagcactttt atcatcaccc
     8761 cgtataatgt ggatatcatg ggggatctgc tggacgagct tgaaagcaac ggcgtgttct
     8821 tctgattttg agaggctaca taccgcccgc ggacgacgat gagcgcttgt tcgtccgccg
     8881 cctgggggac cttgtgcagg tcgtgcgcca gcgcgggacg tcccgcagca ccccgttttt
     8941 aagcgatcgc cagcaggaac tggcccgcgc cgctcttgcg gggttgggct ttgagggata
     9001 tgctttcgac ggcggctatc cggatgccga acggcggatt ctgcgccttt tcggtgaata
     9061 tggggcgcag gagccgctgc ccgccgtatg tctgtttgcg cagaccctgc gggctgacag
     9121 agcactcact caccgggatt atctgggcgc actgatgtcg ctgggaatca ggcgcgaatg
     9181 catcggcgat atccttttgt cggaagacgg agcatatctg ttcgttctgg atacggtcgc
     9241 gccgctggtg tgcgacgagc tttccagcgt ggggcgctgc agcgtatgtg tgcgcgcggg
     9301 ccgggcggag gaactgcccg tccgggaaga gcgcccggca cagacggcga cggtggcctc
     9361 gctgaggctg gacgctgtgc tggccgccat gctgcatatc agccgtgggg atgcggtgca
     9421 gcttgtgaaa agcggcatgg tggaagtgaa ccacgtttcc acggtgtccg cgcattatga
     9481 agtgtttgaa aacgacgtgt tcagcatccg cggacgcggc aagtacaagc tgtgcggcgt
     9541 gggtgccaaa agccggaagg gccgcacctt cgtgtcctat attgaatatt aacaggggga
     9601 acaatctcta tgaacgctga agatatccgc aacgttacct ttgacaaggt catgcgcggc
     9661 taccggcccg aggaagtgga cgcttatctg gaccaggtgg cggcagaact ggaacgcctg
     9721 cagacggaaa aagcggatgc ggagaaaaaa atgtacatcc ttgcggaaaa ggtggaccag
     9781 taccgcaatg atgaagaaac gctgaagaca gcgctcctca atgcccagcg tatgggtgag
     9841 annnnnnnnn nccagaaagc cgagaccatc attcacgatg caacgagcaa ggcaagccag
     9901 gcgcgggaag aagccgtgga aaaggtagct gaagaggaga tgctgctggc ccgcctgaaa
     9961 gcggaggtgg cgcatttcaa gggcgaagtg ctgaacctgt ataagcagca tatcgaatcg
    10021 ctgagcatga tcccgggcgc ggaagaaaaa aagaaaaagg cgccggaggc gcccaaggct
    10081 gaagaacagc ccgagcctgc cccggaggaa gcgccgaagg aagagcctgc ggcggaggcg
    10141 cagcctgccg cagccccggc cgaaccggga caggaggctt cgttcgagga gtatcaggac
    10201 attacttttg aggactaact gaacatgtgt ggcatagcgg cgtttgtttt aagcgccgct
    10261 atgttatggt ttacggcatt tttgcatttt tcggcatttg cagaccattt tctgtggatt
    10321 tgcctgcaaa aacggagaga gtctggcttt tcaaagtgcc aatcaaagaa aggaacgtac
    10381 cattgctgat atctgtaatc attatcattg cactgacagg gctggatcag ctcacaaagc
    10441 atcttgccac ggtttatctt gcgccggtag ggtcgatgcc gttcattccc ggcgtcatgg
    10501 agctgcgcta tgtgctgaac gacggcgcag ccttcagcat gctggcagac gccgagtggg
    10561 ggcgcgtgtt cctcatcgtc gtaacgggc
//
LOCUS       DAWBSB010000065        10531 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1452_length_10531_cov_4.070351, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000065 DAWBSB010000000
VERSION     DAWBSB010000065.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 10531)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10531)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10531
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1452_length_10531_cov_4.070351"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3710..3719
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1946 a         3175 c         3272 g         2128 t
ORIGIN      
        1 aacacatttt tcgatttttt ccagtttccc catacagcat cgacctttct aaggtacgtt
       61 ccgaaagcgg acgtattttt tccttgcata caatgatacg agccatattt gacaaaacac
      121 cggagaaaaa gtgagcaaat tgtgaataaa tattacaaat ttgaaatcgc agcacgaagc
      181 ggcgcggatg ccggacgaaa cggcatatta cggggcgcgg ttttatttca tttggggagg
      241 gcgccgttcg gccttacagc ttttcgcagc ccagcgcggc aagaaaacgg tcgaaggcgg
      301 caagaccgtc cggtgtcagc ttgaacacgc ccgcgtgctc caggcacgtt tcaaacttcg
      361 cgccgatctc ccgtttcaca gcgtcctgcg cctgctcggg ggagagcgcc gtgccgtatt
      421 ttttcaccaa aaggtccagc caatctgcgt gctccggtgc ggcggctctg cacgcttttg
      481 cgtccgtttg gccgcacagc accgccgcga tggcttcgct ctgctgcgca aggcggctgg
      541 gcaggatggc aaggcccatc acctcgatga ggccgatgtt ttcttttttg atgtggtgga
      601 tctcccggtg cggatggaaa ataccctcgg gatactgctc tgtggtgcgg ttgttgcggg
      661 gtacaaggtc cagcacatag cctttttttt cgtcataatg caggataggc gtgacggtgt
      721 tgtggggcgt ggggcttccg ttttccatgg tctcggccag gatgtccgcc tctgcatccg
      781 aatagccgcg ccaggtttcc agcagcctgc ccgcaaagcc gataagctgg gcggcgtccc
      841 ggccgcacgc acgcacggtg ctcacgggcc agcggatggt ctcgacggag atgtccggat
      901 actccggcac acggtagcgg cacagcgttt cggcgctttg catgggaaag gcatagctgc
      961 cgccctggaa gtgcatgtgg ctgaggatgc tgccgcccac aatgggcagg tccgcattgc
     1021 tgccgcaggt gtagtggggg aactgcgtca caaaatcgaa cagctgctca aaggtgcggg
     1081 gcgtgatggc catgggcgca tgctcatggg cgaacacgat gcagtgctcg ttgtaatata
     1141 cataggggct gtactgcaga taccaggtct ctccgcagag cgtcagcggc accacacggt
     1201 gattctggcg cgcgggaaaa ttcacgcggc ccgcatagcc gatgttctcg atgcacagca
     1261 tacatttggg atagctggct gccggctgca ggcgctccag agcgatggtc ttgggatcct
     1321 tttccggttt tgtgaggttg atggtgatct ccagcctgcc gtatttgcag ggatagtccc
     1381 atttgatgtt tttggcgatc tgcgcggtgc ggatatagtt ggatgcgatg cacaggccgt
     1441 agaaatagcc cgtggccgcg cgcacgccct gcgtttgccg cagctgctcg aaccggacgg
     1501 ccgtctcgct ttcccggggc atcaccgcgc ccatgatgcg cgcctcaaaa tttacgcgca
     1561 gagcgggcac ctccccgtcg tacaggccac gggccgccgc gtcatccagc agagcgtcca
     1621 gcagtacggt gggaacgtcc agatgctcct ccggcacgcc gtccgcccac ggctcagaca
     1681 ggccgaacag gtccagcagc gtgttgcgcg ccacataggc gtccagctcg ccgatgagag
     1741 aatgttgcag gctgaattga agcagccgtt cgatcaaaag cgcggtgtgg gccatgattt
     1801 tccctccaaa gtcggataaa atatcgtttc gtacatttta attataggag agtagcagcg
     1861 ggatttccat gtagttttgt tgtataaaac ggcgattctg tcgcgaaacg cgctctgtgc
     1921 ccgcggacgg acccgtctgc ggcttgacaa gcgccgtcgg ggatgatagg ataaatacaa
     1981 ttcaaaggca gtgacggaga aaagtaaggc ggaacagccg gaaaagcgag cgggggaagg
     2041 tggaagctcc gcgccgcgcc ccgcccgaaa gaacacttcc gagccgcaac ccgaacgccg
     2101 ggcatgcacc ggccgtaggc acgccgtggg cagcgcgtta agctgcgggc gctttttggc
     2161 gcagaaaagt ggccgcgcaa gcggtaattt aggtggcacc gcggattcga cagcaattcg
     2221 tcctaaaggt ttgggacatt tggctgtcga tttttcattt tatgaaggag gatatctcat
     2281 gaaaaggaca gagatccgcg cgcttttcgc atccgcgcct gccgacggcg cctccgtcac
     2341 cgtgtgcggc tgggccaaaa cggtgcggga ttccaaaaac atcggtttta tcgagctttc
     2401 cgacggctcc tgcttcaagc cgctgcaggt ggtgtttgaa gctgccaagc tggaaaatta
     2461 caaggacatt gccaaagcgg gcgtgggcac cagccttgtg gtgacaggcc gcgtggtgct
     2521 tacgccgggg gccaagcagc cgctggaggt aaacgccgat tccatccgcg tgctgggcgc
     2581 gtgcccgccg gagtacccgc tgcagaaaaa gcgccatact gtggagttcc tgcgtaccat
     2641 gccgcatctg cgcccgcgca cgaacacgtt cagcgcggcg ttccgtgtgc gcagcgtggc
     2701 ggcttacggc atccacaagt ttttgcagga aaacggtttt gtatacgtgc atacgccgct
     2761 gatcaccggg tcggactgcg aaggcgcggg cgagatgttt cgcgttacca cgctggacgc
     2821 ggcccagccg ccccgaacgg aagaaggcgc ggtggatttt tcccaggact tcttcggccg
     2881 cgctacgaac ctgacggtct ccggccagct ggagggcgag gcgatggcca cggccttcgg
     2941 caagatctat accttcggcc ccaccttccg cgccgagaat tccaacacac cgcgccatgc
     3001 ggccgagttc tggatgatcg agccggagat cgccttcgcg gacctggcgg atgagatgga
     3061 cctctccgag gcaatggtga aaagcgtcat cgcctatgtt ctggagacct gcccggacga
     3121 gatggctttc ttcaaccagt ttttcgacaa aacgctgctg gagcgcctga acgctctggt
     3181 aaacagcgat tttgcccgcg tttcttacac ggaggccgtg gagatcctga gcaagcacaa
     3241 cgattcgttc cagtataagg tggagtgggg cacggacctg cagaccgaac acgaacgctt
     3301 cctcaccgag cagatctata aaaagcccgt atttgtgacg gattacccca aggagatcaa
     3361 gtctttctat atgcgtgtga acggcgacgg aaagaccgtg gctgcggcgg atatgctggt
     3421 gcccggcgtg ggtgagctgt gcggcggcag ccagcgtgag gagcggctgg acgtgctgac
     3481 ggcgcgtatg gatgagctgg gcctttcccg cgcggattat gagtggtatc tggagctgcg
     3541 gcgcttcggt actgtggagc acgcgggctt cggcctgggc ttcgagcgcc tgatcatgta
     3601 tctgacgggc atccccaaca tccgcgacgt actgccgttc ccgcgtacgg ccggcggctt
     3661 ctgagtattt gaaaaggaaa gcggcgcccc gtaccaacag gtgcggggcn nnnnnnnnnt
     3721 tgcgcgtata atcgtcgaaa tgccgctcgt acgggggctc ttcccaagcg ggtacataga
     3781 aatcctcttc ctcaaacacg cctttcggcg caagcgctgc cattttaacc ataccaaagg
     3841 cactcctttc aaacatcgtg gtttctttcg gtgtctttag tataggatat tatatgtccc
     3901 ataatcagga cttttaacgc ctattcgtac tgcgcgagca tctcccggca gaaggcggtg
     3961 tattcccgca ccacggcagg cggcccaagc agctttgcgt ccgtgccgaa gccggccacc
     4021 cagcccatga actgggggct gacgacggcc tgcacatgca cccggaaaaa gctgccgccg
     4081 tcgggcagca gtgtgacctc cttgccgaag cggtccagca ccacgccggc cagccgcgcg
     4141 gcgaatcgca gctccacgtc ctgctgcgca ccgccgaaca tcccgaacat ctgtttggcg
     4201 tactgcgcca tgtcgaagca ttcgaatgtc gccttgccca gcctggactc ttccagaacg
     4261 gacagattcg ccattttgtc cacgcggtaa tggcggatgc ccggcggcga cgctgtgtgg
     4321 tcgtaggcca caagataata gttctcatcg tcccacacca gcgcgtaagg cgagcgctcg
     4381 tagagctggc cgtcccggcg gtagcgcacg gttttttcca tagtccattc aaaataacgg
     4441 aacgcaatct gcctgtcctg tgaaatggcc gtatggatgg tgtcgatgct gtaataaata
     4501 ctctcgttca tgctttttac gcggcccacc gcgtacacct gccgctgcag gctctgggcc
     4561 tcccagcggg aggcgaggcc ctccagcttg cggataagct cacggctttt tttgacggag
     4621 aggaatttgg aggactgtac cgcgtctacc agcagcttca gctccggcaa ctcgaactcc
     4681 cggtgggcaa gctggtagcc acccgctcgg ccgcgggtgg cgatgatatc catgccgtaa
     4741 tcctccagcg cacggagatc atcgtagatg cttttgcgct cggcgtgcac gcccagttcc
     4801 tccagccggg acaggatgtc gctcatagaa agcgcgtggt cttcgtccgt ttcccggcgc
     4861 agcaggtcgg ccaggtacag cagcttcagt ttttgtcctt cgcttttcgg catcctgcac
     4921 atcctttaca atgctttttt gtggaaaaaa ttgaaaattt tttcaatctg ttttatactg
     4981 gtatcatagc atgtcccatg cgggatggca aggaaccgcc gtccgtgcgc gggcgggaaa
     5041 aagaggggga gcgttgcctt gatcaccgat atgtcccagg ggagacccgc cgccatcctg
     5101 tggcggttca ccatccccat gctgctgagc gcggtgttcc agcagctgta taatgtggcc
     5161 gacagcgtgg tggcgggccg gtttgtgggc gaagatgcgc tggctgccgt gggcgcgtcg
     5221 tttcccatca caatgatctt catggccgtg gcgctgggca gcaacacggg ctgctcggtc
     5281 atcatcagcc agttgttcgg cgcgaaggag acagcccgca tgaaaactgc ggtgtccacc
     5341 tcggttattg ccattctggc gctttcggcg ctgcttacga ccggcggctt tctgttctgc
     5401 gagccgctga tgcgcctgct gggcacgccg gaaaatgtgt ttgcggattc ccagctctat
     5461 ttgaatgtct atatcggcgg gctgacgttt ttgtttttgt acaatatttg cacgggcgtg
     5521 ttcacggcgc tgggcgattc ccgcacgccg ctgtatttcc tcatcgcttc gagccttgcg
     5581 aacatcgtta tggacgtggt gttcgtggtg tgttttcaca tgggcgtggg cggcgtggcc
     5641 tgggccacgt ttttgtgcca gggcgtggcc tccgttctgg cggcatggac gctgttccgg
     5701 cggctgcggg gcgtgcccgc gccggagcgg catccgctgt tttcctggcg gatgctgggg
     5761 cgcattgcgc gcatcgccgt tcccagtatt ttacagcaga gctttatctc cgtgggcaac
     5821 ctgttcatcc agagccttgt caacggcttt ggctccgctg tggtggccgg ttattccgcg
     5881 gcggtgaagc tgaacacctt tgcgctgacg ggcttcacaa cgctgggcaa cggcctctcc
     5941 agcttcacgg cccagaatat cggcgcgggc cgtcccgaac gtgtgaagca ggggttccgc
     6001 gcaggcgcgg gcatgacgct gtgcgtggcc gtgccgttca ttgcggcgtt cgtgttcgcg
     6061 gggccgcaga tggtacgcct ttttttgagc gctcccagcg ccgacgcgct ggcacagggc
     6121 acgctgttcc tgaacatcgt ggcgccgttc tatgtgctca tttcgctgaa gctggcggcg
     6181 gacggcgtgc tgcgcggcgc gggctgcatg ggccagttca tgacggctac gtttacggat
     6241 ctgatcctcc gtgtgatcct ggcgttcctg cttgcgccgc gcttcggcgc cgcgggcatc
     6301 tggatgtcct ggccgctggg gtgggcagtg gcggctgcgc tgtcgctggg tttttacttt
     6361 acaggaggct ggcgcaagcg cgtgggcagc atcgacaaat agaaaaaaca aagggcgcgg
     6421 ccatcggccg cgccctttgc agaaggcccg ctgtgctctg aatggattta tgggaagata
     6481 aagcttttac cgccccgccg cccgtgcacc cggcgctgcc gcagggagca cgtcagcggt
     6541 agatgcaatc ttctatatgc gggagcaggg tatagatgga attcccaatg agcgcttcaa
     6601 aatattcctt atactgatac gttttattcc accggtcgat ggaaaacggg tatattccat
     6661 agcgccttgt tgtgtcgatg aatctttttt ccaagatgca aaaaccactg gcgtcggcaa
     6721 gtgcatcgca gagaatgatc agtttgtcat aatcgtcata gtcgaggccg ttcaggaaat
     6781 cccggatgaa ggcgtactgc gctgcgctga tatccttctt tccgatgtcg gcttcaatat
     6841 ccttgacggg gaaggaatgc gtcaggcaga tccttgcgac ctcgtcccat ccccgcgcgg
     6901 cggcgtaatc gtatccgtcg atgatgtgcc tgacagcggc cactccggtt cttcggccta
     6961 tgtcgtgcag cagcccgcag acaaatgcct tatcgctgtc cattttatcg cagcgcccgg
     7021 caatcagctc tgccgcatgc gccacatttc tggaatgttc cccccaggga ccgggattca
     7081 gttcctcggc ctttgccagt tcctggagtg cggtttttcg gtccggaaac atatcggctc
     7141 ctccttttgc acaaatgcat ttacagcccc ggcagagcca tgggcgctgc tttgcgtatt
     7201 tcagacgtcg ttattccgcg cgctcttcgt tcagcagccg ggcgggtacg gtgatatccc
     7261 gccggggcac cttttcccag gaaaagcatg cggccagcga ccggagcggc accggttccg
     7321 gcgcgggctg ctgtccggcc agaaaggcta gccagcccgt cagctccgcg ggcgtgaagc
     7381 gggcgcgcag cgcacccatg tccaggcttt tttcccgctt ggagagccgg acgccctccg
     7441 cgttcaccag cagcggcaaa tggtagtatt ccggcgtggg caggcccagc agccgctgca
     7501 aaaaaatctg gcgcggcgtg ctggagagca ggtcctgccc acgcaccacc tgcgtcacgc
     7561 ccatgcgcgc gtcgtccacc gccacggcca gctggtaagc atagataccg tccgagcgcc
     7621 gcaggatgaa atcgccgcat tcccgggcga ggttctgcga aaaatgtcca agatgtccgt
     7681 ctgtgaaaga aacggactct tccggcacgg caatgcgcag agcgggcgcg cgccgcctgc
     7741 gcagagcgtc cgcctcggcg ggagaaagcc gcgcgcaccg tccgccgtac agtacggcgc
     7801 cgtcggaaag gtgcggcgcg ctggcggcgt gcagatcggc ccgggaacag aagcacgggt
     7861 aaacgcgggc atgttctttc aacatctgca gggcctgtgc gtacagttcg ctgcattcac
     7921 tttgaaaata ggggccgtcc ggcccgcctc tccggccgcc ttcgtcccaa tccagcccca
     7981 gccaggccag gtcctcctcc aaaaggcgcg cgtgctccgg cttgcagcgt cggacgtcca
     8041 gatcttcgat ccgcagtagg aagcgtccgc cccggctgcg ggcggaaagc caggccagca
     8101 cagcgcagaa cacattgcca agatgcatcc ggcccgaggg gctgggcgcg aagcgcccgc
     8161 acacgtcagt ccgttcccgc atggctgttc tccgtcctcc ggacgccgcg cgcgtttcct
     8221 tcctccgtgc cgggcgtgca ctgcgccgaa aaggcgcgca ggtataccgc ggccgagcgg
     8281 gacatctccc gtgaaaaaac gctgttgtat ttgcgcgcgc agaatgcctg catccgcgcg
     8341 gcatagtcca cgtccgcgcg gccttcggcg gcctcgcgcg cgcggtacat ggcctcgtgt
     8401 tcctccggcg tcatgcggcg gtaacggttt tcccgtacga tataccgcgc gtcgaagcgg
     8461 gcgggcttgc gtcgctcccg ctgctgctgc gtgggccggc ccagcaccag catggcagcg
     8521 ggcacggcgt agggaggcag agccagtgcg ctgcgcacat cctcgcagtt ttccagaata
     8581 tccccgatgt agcagctgcc aaggccgaag ctttcggcgg ccaccaccgt gttttgtgcg
     8641 gcgatggccg cgtccgccgc ggccagcagc aggtcgccct cccgcggcgg acggggtgcg
     8701 cagcccgccg cggcaaagcc gtcgtaccag cgctggcagt ccgccacgaa caccagcacc
     8761 acgggtgcgt ccgcgatgaa gggctggtgg tcgcacaggt cggccaggcg tgccttcagc
     8821 gcggcgtcgg tgatgtcgag gatggtgtac agcatctggt tgccagccgt gggggcctgc
     8881 atggccgcct ggaacagcgc gtttttaaca gcctgcggca caggctcctc tgtgaacacg
     8941 cggacggatt tgcgcgcgca aagcgcatcc agtgtgggat tgcgttcaaa ttccattcaa
     9001 agcacctccg gcgcgggacg gcacagggct gccgcgtcgc gcagtgatcg ttccgtttcg
     9061 gcggcgccca gcgcgctcag cggcacaggc ggcacgggca cggcaggaag cggctgcaac
     9121 gtgagctgat aataggaaag acccagccac cggccgtcct tgtagcccgc gtggggcagg
     9181 cgcgcggctt cggcaaagcc catccgctcg tggaaggcgg cgctggccgg gttggggtgt
     9241 gtgatgagcg caaagcagtt gacgtagccc tgcctgcgca gcagagccag caatgcggtg
     9301 tacagcgcgg tggcggcgtg ccgccggtgt gcggcggcag acaggtatac tgtgacctcg
     9361 gcgtcccagt cgtagccccg gcgtgcggcg aagcgaccgg cgtaggcata gcccagtacc
     9421 gcgccgtcct cctccgcaag cagccaggga aagcccgtgt ccagatgctc ccgcagccgt
     9481 ccggaaaatt ccgccaaaga cggcgcctcg tattcccagc tggcggtgga atgctccaca
     9541 taaggcgcgt agaccgccag catgccgccc gcgtcctccg ggcgcgccat gcgcagtgtg
     9601 atgccaggca tcgtatccct cccgtcccgg ccgtggccgg ggtcatgccg ccgggcggca
     9661 gagccgcggc ggttcaagaa atattatacg gcaaagcccc cgcctgcgca atgcgcaggc
     9721 gggggctttg taaaacagac cggctgcctc accggaaagg cccgccggag ccggaaaacc
     9781 gaacaggctc ttgcggcgaa ttgaaaaaga aaggctttaa ataaaatcct gcgaacgcag
     9841 gtctccaccc ggaaggcaca gacgaaagta tgatcagtga gtgtcctgca gggcggccac
     9901 gccctcctca ccggtgcgca ccttcacaac attttccaca ttgtagacaa agattttacc
     9961 gtcgccgatg tgtccggtat acagcacccg cttcgtgacg tcgatgacgc tgcgcacggg
    10021 caccctgctc acgacgacct ccactttcac cttgggcagc agtgtcacgt ccatgggcac
    10081 gccgcggtag aattcgctct gtcccttctg cacgccgcag cccagcacct gcgtcacggt
    10141 caggcccgtc acaccgatgg aggaaagcgc ctctttcagt gcatcaaagc ggctctgccg
    10201 cagcaggatg accactttgc tgatacgcgg cccggatgcg ggagcggcgg tgacggtggt
    10261 ggtttccacc acaggaaccg cttcgtcggc gctttttgcc gggacagcgc cctcaacagt
    10321 ctcctgcact tcagggctta ccatgcccag cgacatgggc gcaaagtccg cgtaggccga
    10381 gatgagcccg tgctcgtgga tgtccaggcc gtcgatctcc tcctcgcggg aaacgcgcag
    10441 gccgatggtc tttttcagca ggaagaacac cagtgtgatg gcaacgccga cccaagcgca
    10501 tacagccagc acgcccaaca tctgtacgcc c
//
LOCUS       DAWBSB010000066        10319 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1479_length_10319_cov_3.584275, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000066 DAWBSB010000000
VERSION     DAWBSB010000066.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 10319)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10319)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10319
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1479_length_10319_cov_3.584275"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1908 a         3447 c         2923 g         2041 t
ORIGIN      
        1 cataatcgtt gccgcagcca tgcatttttg taaaatccag ttttcccata gcaagtgtcc
       61 tcccgttccg aatctcccat acattccagc gggccgcgcc cgctcaacag ccgccttcgt
      121 acatgcgcag gatggcctgg gccacttccc ggcgcgtacg gcgggaatcc atggcctccc
      181 gctcaatgta tttgtgcgcc tccggctccg tcagcagacg gtactggatc agcgcgcatt
      241 tcgcccggtc caccagacga atgtcgtcga tcttttgctg caggatgttg ttctgccgct
      301 gcaaacccat gatgcggcgg ttcgccgcct gcgccagctt gacagcccca aacaaaagcg
      361 cccggttcag cggtttcggc accacgaata cgcctgcgtc ctccacgcgg accgctacct
      421 cgtcggccat ctcggccttc accagcagca ccacgcccgc ccctgtgctc ccggccgcaa
      481 acacggccag ctcgtggccg aactcgtccg acagcggcgt gttgatgagg atgacctgcc
      541 agtccgcgtc ggcaaagccc cgccgcgcct cggcgccgga agtcacggtg gatatgtccc
      601 ccgcgcccat gtccgtcagc actccgcaca gcatatcgcg gcccttttct ccgctggata
      661 cgatcagcgc gcccggcaac gtcctcaccc ccttgcggct ccaaaatatt acattctgta
      721 attctaaagt taaatattac aaaactccga aatatgcttc gtcctgatat ttttccttat
      781 cccgcgctgc gaaccagcgg ttctgctccg cctgcttgag tgccagatat ttgcgcgccg
      841 tatccgccga gagcactttc tcaacaaagg ccgaaccgcc ggcccgcgca atggcctcct
      901 ccagcgaggc gggcagcgcc tccagcgcgc cggcgtttgc cgtgtacagg tcgtagggcg
      961 tctcctcggc aagctccatc ttccgttcca taccctcgaa gcccgctgcc agcagcaatg
     1021 caaaggccag ataaggattg cacgagacgt ccggcatacg cacctcgatg cgcgcatact
     1081 cgggcgcggg cgcggccggg atgcggatga gctggctgcg gttctggtgg ctccacgcca
     1141 catagcgcgg cgcctcgaag ctgccgaacc gggcatagct gcccggaacc ggattcgtaa
     1201 aggcgcatat ctcgcccgcg cgggccagca cgcccgcgat gaacgaacgc atggccgggc
     1261 tgtacgcgcc gtccggccct ggagcgaaga tgtttcggcc tccccgtgcc agcgaaatat
     1321 tcacatgcat gccgctgccg ggcttatccg ccaggggctt gggactgaac gagccgtaca
     1381 gcccgttgcg cgcggccacc gtcttgacca catttttaaa cgtgatgacg tcgtccgccg
     1441 ctgtaagcgg tgcggcgtgc ttgaaatcca cctcgttctg gccggggccc tgttcatgat
     1501 ggctggattc cggccggatg ttcatttcct ccaacgtcag gcagatctca cgccggacgt
     1561 tctcgcccct gtccaggggc gcgacgtcaa aatagccgcc gttgtcctgg ggacgcttgg
     1621 tgggcctgcc ctccgcgtcc cgctcaaaca ggtaaaattc acattccgcg cccacacggc
     1681 actcgtagcc tgcatccgcc agacggcgca gggtcctgcg caggatatgc cttccgtccg
     1741 ccccgtaggg gcttccgtcc gccgtgcgca cactgcagaa aaaacgcacc acgcgccccg
     1801 tctgcggccg ccacggaagc accgagagcg tggccgcgtc cgggaacagg aacaggtccg
     1861 atttatctac gggcgcgaag cccaaaatgg acgatgcgtc gaaagaaacc cccgtttcga
     1921 acgcgcgcgg aagctcgctg ggcatgatgg cgatattttt cagcgtgccc atcacatcgc
     1981 agaacgccag tcggatgaac tttacgtcgt tttcctccac aaactggagc acggcttgca
     2041 tcgtcgaatc cattgttttc cctccggttt ggccgcgccg tgcgccgcct tgcttttttg
     2101 cgcgccggct gttgccggaa ccgcaacagg cggggcaaag ccccgcccgg tgcgcgccgc
     2161 gcgttgttac gaatagcagt acaactgccg gtatgggttc tgcgtgttgg gctccagcag
     2221 gaagaaattc tccctcagga tatcctcctg cgccagcccg aacacgccgc agtattccgc
     2281 cagatacggc tccacggccg ccaggtcatc cttcccggcc ctgcgggccg ccacttgggc
     2341 gggaaggtcc gggggcagcg tgtcgcagcg cagcagcaat ctgcccccat gcatgcctgt
     2401 gcccacaaaa cggcccaccg gcgctctgcc gccgctgccc agccccaaca cgataataat
     2461 gccgccggcc tggtattcgc ccagaaagct gcccgcctcg ccgccgatga tgagcaccgg
     2521 ctgcttttcc cggtacgcct tcatatggat gcccgcacgg tagcccgcgt tgccccgcac
     2581 gtagattttc ccgccgcgca tggcgtagcc ggtggcatca ccggaagaac catgcacgaa
     2641 gatcgcaccg tcattcatgg tatcgccggt ggcgtcctgc gcgttgccca gcaccgtgat
     2701 gcgcgtgccg ttcagatatg cgcccagcgc gtttccgggc gtgcctgtca gcgtcagctc
     2761 tttctttcca aggcccgccg caatatagcg ctggccgttc acatgctcca cggccagcgc
     2821 cgtgtccgtg ctttcccgga tgcgcgcgca aagctccttt gctggcatgt ttttcgcgtc
     2881 aagctttgtc atcacacttc tcctcccagc tgtaccctgc ggtccttttg ggaaaacgcc
     2941 atgagctgcg gcgattcccg gatcaggtca aagtcgatct ccgacagcgg ctttttcttg
     3001 cggatgaggt ctgtataaat accggcgcgg gatacatccc ccaccatgat aacgcccttg
     3061 agaacgccgt ctgccgtgac aaggcgtttg tagctgccgt ccctctgcac cgtgaagctc
     3121 tcgccctcat aactgcccgc cgtaatcatg tgcaggccga acacgcccga agcgttgagg
     3181 gcaatgccct gcgtgtaatc ggcgcggccg cccgccatgt tgatgcccgc cgtctcgccc
     3241 tgcagcacgg cgttgggcca caggggcagc atgcgctttt cgccgctcac cgcatcgtat
     3301 ccctgggcac agtctcccgc agcatataca tcgggaagcg tggtggcaca gcggccgtcc
     3361 accaggatgc cccggtccac cgcgccgcct gcgtccgcca ccagctgcgt gttcggccgc
     3421 acacccacgg ccgttaccag cacgtcgaat tccagctccg ttccgctttg gagccgggcc
     3481 gtgtttcccc ggaatgcggc cgcgctgtcg ttcaggtaaa aacggacgcc cttctcctcc
     3541 atacgggcct gcaccatggc agcccccgtg tcatccagta cagcaggcag cacgcggggc
     3601 gcaaggtccg caatggcgat ctgcgcgcac aggccgcgga tgccctcggc gcatttgacg
     3661 cccgtgaggc ccgcgcccag aatgagcacg cggctctccg gccgcagcgc ctcagccaga
     3721 gccgaggcgt cggacagcgt ctggaagcaa aaggtacgct ccaccgtttc cagcccggga
     3781 atgggcggca cgaacgggcg gctgcccgtg gccgccagca gcctgtcgta agccagccgc
     3841 tcgcctgtag agagccgcac ggtcctgtct ccggcatcca gcgtggtggc cagcacgccg
     3901 gctttcaccg tcacaccgtt ccgggtaaaa aagctgtccg gccggcacca cattttgtcc
     3961 cgcgtggtct tgccctccag cagatacgaa atgagcggac gggaataatt ccactccgtc
     4021 tctcctgtaa tgagaatgat ctcgccggtt ttgtccacgg agcggacgcc ctcgatacag
     4081 ccgatggctg ctgcgctgcc gccgatgatg acgtacctca tgccccggcc cccctctcct
     4141 caaagacgat ggccttgttt gggcagtgct ccacgcaggc gggtacaccg ccgctttggg
     4201 tgcacagctc gcacttctgt actgcgccgt gcgcgccggg caggacagcg ccataggggc
     4261 aactggccac acaggtgtag cagcccacac atttgctttc gtcgatggtg acgacgccgt
     4321 cccgcacctg cagcgcaccg gagatgcagc cccggacgca cagcggctca ctgcagtggc
     4381 ggcatgccac cgcgaagctg atgcctcccg gtccctcctc gatacggatg ttcgggtgca
     4441 cggcggcgcc cttgagcgcc ttcaccatgt cgcttttgcc cgaattggca aacgcgcagt
     4501 aatactcgca cagatggcac ccaaggcacc actgttcgtt cacataaaca cgtttcattc
     4561 actttccctc gttcccatgc atgcaggccg ggcccctctt cccagccgta cgcgccatcg
     4621 aaacggaagg cgtgccgatc aaagcacgat atactggtcg cgctccgcgc caacggagac
     4681 atatttgatc tttacgccga ccgcttcttc gataaatgcg atatactcga gcgcctcccg
     4741 cggcagctct tcttttttcc ggcagccgga aatatccgtc cgccatccct tgaacatttt
     4801 gttgacgggc ttcgcctgct ccagcacatc cccatagggg aaatcatgaa tctcccttcc
     4861 gtccagctca taggccacgg taacggggat ctcatccagc gaatccagga tatccagctt
     4921 cgtcagcgcg atctcgtccg cgccctgggc gcggcagccg tagcggctgg ccaccacgtc
     4981 gaacgggccg acacggcgcg ggcggcccgt ggccgcgccg tactctccgc cggcggcacg
     5041 cagcttctcc gcctcctcgc cgaacagctc gcaggtgaaa gggccctcgc ccacacagga
     5101 ggaatacgcc ttcatgatgc cgatgttccg gtcaatctta tggttcggga tgcccgcgcc
     5161 cacgggcgcg taagcgccga tgacgttcga cgacgacgta taggggtaga tgccgaagtc
     5221 gatgtcgcgc agcgcgccca gctgggcctc gaacaggatg tttttccccg ctttggccgc
     5281 tttgtccaga tacagcccag cgtcgcagat ataaggcttg aggatgtcgc cgtattccac
     5341 gagccacgcc cacatatcat ccgcgctgac cggcggcctt ccgtacacgc cctctatggt
     5401 gagatttttg taggccacga tatccgccag gcgcgctttt gtcgtctccg ggtggaacag
     5461 atcgcccatg cgcagggcct ttttcatata tttgtcgccg taaacgggtg cgatgccgcg
     5521 gcgtgtggag ccgtatttcg catccgcaag acggtcctcc tcgtagcagt ccagtagctt
     5581 gtggtacggc atgcagatga ccgcccgctc tgagatcttc agattctccg gactgattgc
     5641 aatgccgccc ctgcgcaggt tttcgatttc gtgtaccaga tgctccagat caatgaccat
     5701 accgttgccc atcacgttga cggtggtctc gcgcaggatg ccggagggca gcagattcag
     5761 gataaatttg cctttttcgt tgataacggt gtggccggcg ttgtttccgc cctgataccg
     5821 cacgacaata tcgtactgtt cgctgagcag gtccaccata cggcccttgc cctcgtcgcc
     5881 ccagtttacg ccggtgatcg ctgttaacat aatacactct ccctttctcc ctttacgggc
     5941 cgccgcagct gcctggcggt ccgtccgcaa aaatttccgt acagttttat gataccatgt
     6001 ttccaagctc acacttcgcg tcccgcccgt gttctctaag caacacggta tctataccgc
     6061 gaaacggcct caacaaaacc gcacctgctg cggttttgca tggttcaatg ttcccaaagc
     6121 cctgtgcgtc agcacagggg cgtgtgcagc gcacacgcgc agccgcgtaa gcggcagttt
     6181 tggggttatt ataccataca gaaggggaaa atactattcg ccggcgtgag aaatccccag
     6241 gatctccagc tctttttctg tcattcccac cccccgcagc atcaggcggt tgcccttgag
     6301 cgcctcaatg gagttgatcc ccataccgcc catcatctcc ttgatctcat gctgccaggc
     6361 ggtgacgagg ttcaccagcc gcgcggcgcc caggtccggg ttcagccgct tgacaaggtc
     6421 cggcctctgg gtggcgatgc cccagttgca gcggcccaac tggcaggtcc ggcacaggtg
     6481 gcaccccaat gccagcagtg cgctggtagc gatatacacc gcatccgctc ccagcgccac
     6541 ggctttgaca acgtccgccg atgagcggat ggagccgccc accaccaggc tcacatctcc
     6601 gcggatgccc tccgcccgca ggcgggaatc cacagccgcc agcgcaagct cgatggggat
     6661 gcccacatta tcccggatgc gggtgggcgc cgcgcccgtg ccgccccgga agccgtcgat
     6721 ggcaatgatg tccgcgcccg agcgcgcaat gcccgaggca atggccgcca cattgtgtac
     6781 ggcggctatt ttcacaatga tgggcttttc ataatgggtg gcttccttca ggctgttgac
     6841 gagctggcgc aggtcctcga tggaatagat gtcgtggtgc ggcgcggggc tgatggcgtc
     6901 cgctccctcg ggcaccatgc gcgtgcgggc cacatcgccc acgatcttcg cgccgggcag
     6961 atgcccgccg atgcccggct ttgccccctg ccccatcttg atctccacag ccgcgcctgc
     7021 gttcaggtat tcgctgtgta cgccgaacct gccggacgcc acctgcacga tggtgttggg
     7081 gccgtaacgg taaaaatcct catgcaggcc gccctcgccg gtgttgtagc acacacccag
     7141 ctccgtagcc gcccgcgcca gagattcatg ggcattgtag ctgatggacc catagctcat
     7201 ggcgctgaac atgatcggca cggccagctc gatatgaggc gtttttttcg gtacgatggc
     7261 accgttttca tcccgccgga tctccatatc ctttttgccc aggaaaaccc gcgtctccat
     7321 gggttcgcgc agcgggtcga tgggcgggtt cgtcacctgg gaggcattga tgaggatacg
     7381 gtcccagtag ccgggcagcg gttccgggtt gcccatgctg gaaagcagca cgccgcccgt
     7441 ggccgcctgg cggtatacgt tttggatatt gtccttcgtc cagtttgcgt tctccttgaa
     7501 cgtgtggtcg gtgcgcacga tcttcagcgc ccgggtggga cacaggctca cgcacctgtg
     7561 gcagttgacg catttcgcgt cgtccgccgt catcatttgt tcatcggcgt catagcggtg
     7621 cacctcgttg gcgcactggc gctcacatgc acggcatgcg atgcagcgtt ggtagttgcg
     7681 caccacctcg tactccggat agataaaatc cgcgtacatt ttcccattct cccctttcct
     7741 acactgtcac gccgtcgttc aggcgcacaa caacggcctg gccgccccgg ggcgcccaaa
     7801 gcgtttcaac gtccggctcc atcacgcgga tggcgcactc ctcgctggca aaatacacgg
     7861 cctcgccctt ttcagcgcac accatgctgc gcagcttgag gcggtcgttc aacgccatca
     7921 tgccgcccgt gaagcccagc aggatggaga atggtcctgt gatgagcagg gacgcgaagg
     7981 tctcccgcag gtaggccagc cgttcccgct gcgcttcggg catctgttcg atggtgctcc
     8041 agaacggtgc ggcgataacg ccggccgctt cttccagcgt caggcccttg cgccgtacca
     8101 ggaaatcaat gatataggta atcacctcgg tatccgtgag caagctgcaa ttatatccgt
     8161 acatctcgat aaagcggcgg tttgcgtcat agctggagat ctcgccgttg tgcaccacgg
     8221 aataatccag aatggaaaac ggatgcgcac ctccccacca gcccggcgta ttggtagggt
     8281 aacggccgtg ggccgtccat gcgtagccct cgtattcctc cagacggtaa aattccccca
     8341 cgtcctccgg gtaacccacg gccttgaata cgcccatatt tttgccgcag gaaaacacat
     8401 atgcgccgcc gatctcccgg tttacccgcg tgacgaaccg caccacgaac tcccgttcgt
     8461 ccagcttgga gtccagcagc gtctgaggct tgggcgcggc aaaatagcgc ataaggctgg
     8521 gcgcgtcccg gatggctgca atggggcgcg tgggcagcgt gccgctgctt tccacatcga
     8581 agtcctgcag caacctcgcc tcggccgcgt gctgggcgtc tacggtgtcg tagaacacat
     8641 ggaatgcata cagctcccgg tattccgggt agataccgta cccggcaaac ccgccgccca
     8701 ggccgttgga gcgctcgtgc atggccgcga tgccggccac gatgccgctg ccgttttcgc
     8761 ggcggccgtc cttgtggaac acgcccgata tggcgcagcc cgacgggatg cgtacctctc
     8821 cttctttcag cataaacacc attccctttc ccgtttcccc gcggggcggt ccgtaccgcc
     8881 ccgcggttct cctattcaaa gcacccctca gtgcttttcc gcgcggcgca gccgccggaa
     8941 aaacaaaaaa cgcccgccag ggggcaccag ccccttggcg aacgtctttg ttcattttta
     9001 tcatattaaa ctttatgcag aatgtcaacg tgccgcgtac gactttgtct gttttcacaa
     9061 aagctgtttt tgaacggcgg acgcgatggt aaaaagcaac agaaatgtcc ggtgcggaca
     9121 gaaatttcga aaaaagcgtt gacagccgcc gcccggaggt gtatgatttg ggccatagaa
     9181 cagcaaaggc gctgtggacg gtttcgtcca cagcgccttt tttgtctgca ttcaggcagg
     9241 cagaggctgt ccgcagggaa agtatacaaa atacaccgga gaaaaggagt aagggagtat
     9301 ggcagcgacg actacggaaa ttttcggcag catggtattc aacgaccacg tgatgaaaga
     9361 gcgcctgccg agcgcgactt ataaatcttt gcatgagacg atccggcgcg gtgcgccgct
     9421 ggacatggaa gtagccaacg tggtggccaa cgccatgaag gactgggcca ttgagcacgg
     9481 cgccacgcac tatacccact ggttccagcc gctgacgggc gtgaccagtg aaaagcacga
     9541 cagcttcatc agccccacca aagacggccg cgttattatg gagttttccg gcaaggagct
     9601 tgtgaagggc gagccggacg cgtccagctt cccttccggc ggcctgcgcg ctacggccga
     9661 ggcccgcggc tacaccgcct gggaccccac ctcctacgcc tttgtcaagg acgaggtgct
     9721 gtgcatcccc accgcgttct gcagctattc cggcgaggtg ctggacgaaa agacgccgct
     9781 gctgcgcagc atgtccgcca tcagcacgca ggcctgccgc atcttaaagc tgttcggcaa
     9841 aacggtgcct caggtctcca ccaccgtagg gccggagcag gagtatttcc ttgtggacag
     9901 ggggcagtac ctccagcgcg aggatctgcg cctgtgcggc cgcacactgt tcggcgcgat
     9961 gccccccaag gggcaggagc tggacgacca ctacttcggt gtcatcaagc cccgtgtttc
    10021 aggctatatg aaggagctgg acgacgaact gtggaagctg ggcgtcgcag ccaaaacgaa
    10081 gcacaatgag gtggcccccg cccagcatga gctggcgccc cttttctcga ccaccaatat
    10141 cgccgtggat cacaatcagc tcactatgga gatgatgaag cgcgttgcca cacgccacgg
    10201 catggcctgc ttattgcatg aaaaaccctt cgacggcgtg aacggcagcg gcaagcacaa
    10261 caactggagc atctccacac ccacggaaaa tctgctggac ccaggcaaaa cgcccatgg
//
LOCUS       DAWBSB010000067        10288 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1483_length_10288_cov_3.522623, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000067 DAWBSB010000000
VERSION     DAWBSB010000067.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 10288)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10288)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10288
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1483_length_10288_cov_3.522623"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1128..1137
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2600..2609
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2007 a         3253 c         3071 g         1937 t
ORIGIN      
        1 gagccttttg gaacacggaa aaatggcaga tgtaattgca gcagttgagg taatccggcg
       61 caaaatccgg tttgaagtgg cctgccgtgg gccggcgcac gtccgaagtg aacaacgcct
      121 cgtcgctgta cagaaacgcc gcgcccgtct cgtgagcggc cttcatcatc tcgtataccg
      181 catgaggcgc cagcacgtcg tcgtggtcgg caaggcccag gtactccccg gcggccagcg
      241 cggcggcggc gttggtgttg gccgagatgc ctttgttttc caccttcgta tatacgatac
      301 gcgcatcccg ggcctgatag gcgcgcaccg tttgcgccac atcaccgtgg gccgcgtcgc
      361 tggcgtcggc caggcagagc tgccaattcg ggcaggtctg gcccaacacg ctgtccagca
      421 gctggcgcag gtatttctgc ggcgtattgt acagaggtac gcagatgctc accgcaggcc
      481 agcccagcgc gccgtaatcc agcgcgcgct gggccgccag cgtttccttt ttgggcagaa
      541 aacgcccttt tttgctgccg aagcgccgct tcaaaaatgc cgccgaacgc ttgagcgtgg
      601 ggccgaagcc atccgtgcgc accgtctggg caaagaagcc cgccagctcc cgcatgcgct
      661 tgattttcaa attcatggat aatctccttt ttaacaatgg ccagccttgc cttttatgcg
      721 ccgccgtgaa gcgctttgcg cggccgttca aacagcgctg cgggccaaag gccgtccctt
      781 tatgaactat gaacgcgccc cgaaccggcg cggcacaaag acgcacgcgg cccggttccc
      841 gcatccgccg ctgcgcaggc aatgccgcaa gcggggccct gcggctcttt caggtcgcgt
      901 attttgtata attttcttta tttataccca tagttttcat ataggagcgt aaaatgcgga
      961 ttataatacg ggtcgccctg ctgcatcagt ccagcgtatc tggcacaaaa gcgcgctttt
     1021 tccctttcgt accgggcctg cttctcgccg cccgcgcggg tatcgtcgcc ccggctcttg
     1081 ctctcatggt gcaccgccgc cgcaaagggc gtcattacgt tcagcagnnn nnnnnnngcc
     1141 acagccgcag gcaaaagtcc acgtcattgt acgctacggc gaaattttct tcgtccagcc
     1201 cgccgaagcg gtcgtaaagc tcttttttca ccatcaggca cgcgcccgtc accgcgcaca
     1261 tattctgcgt ggtcgcaagg cggtacatat cgccgccgct gtcccgcggg tggcccttat
     1321 ggctgtgtcc ggcgctgccg cccaaaccga tgaatacgcc cgcgtgctgg atggttccgt
     1381 cggggtaaaa aagcttcgcg cccacggctc ccacgtccgg ccgctgggaa taagacagca
     1441 gctcccgcac gaagccgggc gtttccacct ggatgtcgtt attcagcagc agcaggtgtt
     1501 cgcccgtggc atgccgcgcg ccgaaattgt tgatggcgct gaaattgaag ccgcctttgt
     1561 aggtgagcac gcggcagttc ggatactgct gctgtgcccg cccgtaacag gcgaacgttt
     1621 cggcctgtgt ggaattgttt tccagcacca gcacctccag agcgtcccag ccggcgttcc
     1681 ggtacaggct ttccagacag cgggccaggt cctccgaatg gtccttgttg gggataagca
     1741 ccgtcacgcg gccgggccgc gtgacgtcat accgggtgcg gtatgcgccg gggctgccgg
     1801 gaatgggttc cactcggccg ggcatgccca ggcgcagaag ctgggcctcc accgccgccg
     1861 cgcccgccgc cagcgcataa ggctttgcct gcatgccgct ggcggtggag ccgccgtgcc
     1921 cgcgccacca atacagcact ttgggaacgt gcgccacctt ttgggcgcgc tccgtcaagc
     1981 gcaggatcag gtcgaaatcc tgcgcgccgt caaattccgc gcgttcgccg ccgcccgccg
     2041 ccgccagcag cgcacggctg aatacggcca gatgcgtgat gtaattgcag ccgcgcagcg
     2101 tgtcggggga aaagtccggc ttgaaatggt attcgcccag ctttttcagc gtggaatcca
     2161 gcacgatctc atccgaataa acaaagtccg ccccctgctc gttgacggct ttcaccgcct
     2221 cgtacagcgc attgggggtg agcacgtcgt cgtggtccag caaagcgata tactctccct
     2281 gtgccgccgc aagggccgcg ttggtgtttt cggcaatgcc gccgtttttg gcaaggcgga
     2341 tataacgcac gcgcgcgtcc cgcacggcac gcacagcctt ttccacctcg ccgtgggccg
     2401 cgtcggatgc gtccgccagc accagctcca ggccggcgta ggtctggccc agcacgctcc
     2461 gcatcatttc gcgcaaaaat ttgggcggcg tattgtacaa cggcaccacc accgacacaa
     2521 gcggcatgcg ggcaaagcgc gccgcgcgct gggccttaag ctcccggcgc agcgggatgt
     2581 cggcgcggaa acgccacacn nnnnnnnnnc gccacacctc gccctttgtc atcaggctga
     2641 tgcggaacga cgcctcgcgc cacagcgcct cggcgccgcg ctcccgcagg caggcggcgg
     2701 cgcggcgggc aaggcttgca aaatgagccg ggcgcgccgt gcggcgcaga gcttttgcaa
     2761 aggaatcgtg ctccgccggg tttttggggg tattttcttc tggcatacgg cttatccctt
     2821 tccggtttca tcgaagacag accgcacaat ttacaccctg agcttcaggc actgtgccgc
     2881 catggctctc cgccatgttt cgcaaaaatc tcattgcgct gtcggcgtcg gaattgtgcg
     2941 gtaccgtctt atgacgcggc gcggcccgga cggatgcgcc ggccccgcgg cccgcgccgg
     3001 ccgccgagag ccgcgccggt gatgtgcgcc tttacggcgc ctcaatgatc ctcttgttgc
     3061 tgttcgcgtt ctgaatggct gcggcctccg ggtccagcgc cttgtatgcg gcgcacacct
     3121 cggccgcttc gccctgcatc tttacgcggc ccttttccag ccatgtcacg tggtcgcaca
     3181 tgcgctcgat ctggccgatg gagtggctca ccagcagcac cgtggtgcct ccgctcatca
     3241 gctggcgcat gcgctcctcg catttttcct gaaacatgaa gtcacccacg gacaatatct
     3301 cgtccgcaat gagtacgtcc ggcttggtga tcgtggcgat ggcaaacgca atacgcgcca
     3361 ccatgccgga ggaatagttc ttcagcggaa cgtccagaaa ttcccgcagc tcgctgaaat
     3421 ccacgatctc gtcgaatttt tcgtcgatgt atttgggcgt caggcccagc accgtgccgt
     3481 tgagatagat gttctcccgg gcggtgaggt ccatgtcgaa ccccgcgccc agctcaatga
     3541 gcggcgcgat gctgccgtgc accaccacat tgcccgccgt gggcttgaac acgccggaga
     3601 tcaccttcaa aagcgtggat ttgcccgaac cgttcagccc gatgatcccg tggaactcac
     3661 ccttatgcac ggtgagggaa acatcgcgca gcgcgtaaaa ttcttcaaac tgcagcttgc
     3721 cctgcacggc ggccagaaaa tactctttca ggctctcgtg cttttccttt gccatgttga
     3781 agcgcatgga cacgttgttt acttcgatca ttttcacagc ggcgtcctcc ctcagatatg
     3841 caggacaaat ttgtcctgct ggcggcggaa cacgaacagg cccacagcca gcgcgatgac
     3901 cgcacacagg ccgcagatcc agaccatgct ccaggtgagg cccgtgccgt ccagcaccgt
     3961 ggcgcggaag cccgtgatgt accagtacag cgggttgaaa cggatgacct gggaaaggct
     4021 cagctccacg cttcccacca ccagcggatt catctgctca gggtcataga aaatggcgga
     4081 aaaatagttc agcgccagcg taaacacacc ccacagatgc atcacatccc gaaagaacac
     4141 tgttgccgcg gccagtgcaa ggccgatgcc cagtgtgaat acaaacagtg tcagcagcgg
     4201 ataaaacgcc agaagcacgg tggcgtgcag cttggctcct gttacaagca ttacaataac
     4261 gagagcaatg aacgagaaca gacagttcac caaagcaaag cacacccgct ccagcggaaa
     4321 gatgtatttt gggatataca cctttttgat gagcggcgcc gcgctcagca tgctgcccat
     4381 ggcgctgccg gttccttcat tgaaaaagtt gaacagcagc tgcccaatga gcaaatatac
     4441 ggaaaaattt tcgataccgc tgccacgcat gtcaaaaagc gtggaaaaca ccaccaccat
     4501 gacgacattc atcagcagcg ggttgagcac gctccacacc acgcccagca cgctgcggcg
     4561 gtatttcagc ttgaaatcac ggccgatcag gttctgcagc aggtagcggt accgccagaa
     4621 atctttggcg taagccttca gcatctgcat cccggtttac cacctttcaa agaattcata
     4681 ggtctgctcc ttgaagcccc tgtgctcccc atccttggcg gaaaggcgga tctcgccgtc
     4741 caccgcgggc cacacgatgc ccacgtccgg gtcatcccac ttcacgccgc cctcggccgc
     4801 ggggtcgtaa tagtcggtgc atttgtaagt aaactccgct tcttcggaca gcaccagaaa
     4861 gccgtgggca aagccctgcg ggatataaaa ctgcttctta ttttccgcag agagcgtcac
     4921 gcccacccat ttgccgaagg tctcgctgtt cgggcggcag tccaccgcaa cgtcgaacac
     4981 ctcgcccgac accacgcgca ccagcttgcc ctgggtatgc tccttctgga agtgcagccc
     5041 gcgcagcacg cccctggtgc ttttgctctg gttgtcctgt acaaaggggc cgggaatgcc
     5101 cgcggcagcg aattcctccg cctgataggt ctccataaaa taaccccggc tgtcgccgaa
     5161 caccgtaggt tcgatgatga tcacgccggg gatctcggtt gcgataaaat tgaatttgct
     5221 cataaacaag aacagtcctc tcctgttttt tcattccgca gcaggcccgt ccgcatggaa
     5281 caagccaagt atagaatatc ataaaacggc cccgcgttca aggtatcccg cgcatgcggg
     5341 cttccaaagg ccgcgcgcag caaaacgcgg cgcgcggaca acgggtcttc ccgtccgcgc
     5401 gccgccgttc gtgcgcagcc ttattctacc ggggcgaaat attccgccgg gtccacataa
     5461 gcgccgtcct ccaggaactc cagatgcaca tgcgtttcca tggcgctctc ggcgggcagc
     5521 tcgcccaccg tgcccagtgc gtcgcccacg gccacggtgt cgccctcttt cacagcgatg
     5581 tccgcaaggc cgcatacgcg taccgtcagc tttccggcgt tcagctccac aacgccgccc
     5641 cactgggggt cctcataagc gcgggccacc acagcgccgg tgggcgccgt tacggcgtcg
     5701 cccgccgcgc cggccaggtc catgccgttg tgggtgcgcc agtccttcat ggtgtcgttg
     5761 aacaccagct cgtcaccact gaaagcctgc aaggccgccc cagcccgcgg cgatgcgaac
     5821 gagggcgttt gcaccggctg cgagcttgca ggcgcggcgg gcgcggatga agcaggcgat
     5881 gagctgggcg cagatgaaac cgtactttgc ggcttcgatg acgaggatgg cgatgtgggc
     5941 gccacgggca cgtcctcctg cttttcttct acgggggtat gctgttcttc ctgccatgga
     6001 atgtcctcct caccttggat ctgcggcgcg gggtccgcgc ccagtttgtc catcatgctg
     6061 ttgatggcaa ggaaactggc cgtggccgcg cccgcgatga cgagcgtcaa cgccagataa
     6121 aagcccttgc ccttccaaaa gcttttggat tcctgattgt ttttcatatt tttcctccct
     6181 gccgtgttgc cgccctggcc cgcgcgcggc gcgctggctg cggctgcggc acatgatagt
     6241 agtattttgg acaggcgcgc cccgaatata catgccgccg gccacgcggt acggccgcaa
     6301 aaaaatttac atttccgccg caccgtgcgt tttagccggg cttgcgtttt taatcttaaa
     6361 aaagtacaat aatgctatat acttcccggc cgcatgccgc ccgtttgcgg ggcgcggcct
     6421 tttcagaaag gaggctcctc ccatggcggg atacattgcc gggatcggcg gcgcgaacgt
     6481 ggacatccac ggccaaagcg acgctcccat cgtaatgcgg gattccaacc ccggccgcct
     6541 gcatctttcg gcgggcggcg tgtgccgcaa catctgcgac aacctgtgcc gtctgggcct
     6601 gacggtggag ctgtgcagcg ctgtgggcgg tgatgcatac ggcgacatgc tgcgcggcgc
     6661 ctgcaccgcc gcgggcatcg ggacgcggca tctggccgtg ttccccgccc agaatacgtc
     6721 cgcctacatt tccatcatgg acagcgaagg cgacatgctc gtagccatga gcgacatgca
     6781 tattttaaaa gaggctctga cgccctcgta tgtggagggg ctgctgcctg tgctgaacgg
     6841 tgcggacgtc tgcgtggcgg acgcaaacct ttcggccggc gcgctggaaa cgctcgccac
     6901 acgctgcacc gtcccgctga tgctggaccc cgtatccacg gcatgggcgc gcaacgccgc
     6961 gccgctggcg ggatacttcc acacgctcaa gcccaacctc atggaaatgg aagtgctttc
     7021 cggcatgcgc atcacaaacg aacgcgagct ggacgccgcc tgcggcatgc tgctggaaaa
     7081 aggcgtgtac caggtgtttg taagcctggg acgcgagggg ctttatttca ggcggcaggg
     7141 ctgcggcgcg ctgcgcaaaa aaagccgccc cttccccatg aaaaacgcca ccggcgcagg
     7201 cgacgccaca atggccggta tcgtgtatgc atgggtgaac ggcctttcgc ccggggacac
     7261 gctggatttt gcgctgggcg cgggccttgc ggccatcgac agcgccgata ccatcaaccc
     7321 ggcgatgagc cccgcgctcg tcgccaagct tgtaaaggag tatgtattat gaatttgaaa
     7381 gaatatctgt ccatcacgcc ggaaatctcc gccgccatcc gtgacggcaa gcccgttgtc
     7441 gcgctggaga gcacgatcct ctcccacggc atgccctacc ccgacaacct tgcctttgcg
     7501 gccgaggtgg agcgcatcgt gcgggcggag ggcgcagtgc ccgccaccat ggcgatcatc
     7561 gacggcgtgc tgaaggtggg ccttacgcag gacgaactgg agctgatgtg caaaggcgaa
     7621 ggcgtggcca aggtatcccg gcgcgacctt cccatcatgg tggcggaaaa gcgcacgggc
     7681 gcgacgacag tagcctccac gatgatcctt gccagcctgg cgggcatccg cgtgttcgcc
     7741 acgggcggca tcggcggtgt gcaccgcggc gcggaaaaga ccatggacat cagcgccgac
     7801 ctgcaggagc tacgccagac ctcggtggcc gtggtgtgcg ccggcgcgaa gatgatcctg
     7861 gatatcggcc tgacactgga gtatctggag accatgggcg ttcccgtgct gggcctgcgc
     7921 acggacgact ttcccgcgtt ctactgccgc aggagcggct tcggcgtaga tcacaatgcc
     7981 aaagacgagg ccgagattgc ccgcattgcc aaaacgaagt gggacctggg cctggcgggc
     8041 ggcatactca tcggcaaccc ggtgcccgag gaatatgcca tggactttga cgagatgaca
     8101 gccgtcatcg aaaaggccat tgccggcgcc aacgccgagg gcgtgcgcgg gaagaacatc
     8161 accccctacc tgctggcgca catcgtggaa tacacaggcg gccagtctct caagaccaat
     8221 atccagcttg cgtacaacaa tgcccgagcg gccggcaaaa tcgccgtggc ctacgcccgg
     8281 ctgtaaacgc ccgccgccct gcggcggctg aaaatcaaga aaacacgagg aagtgcaatg
     8341 gacacctgca aaaactgcgg caagccgctg gaaaacccgg accaggagct ttgcccggcc
     8401 tgccgcgccg tggaaaacgc aaaggcgcgg accaggcggg aggaaaaagc cgggcatgcg
     8461 cgccgccggc gcatgaaccg caccacacgc aaaatgctca tcgccgtttg cgtgctcgcc
     8521 gtggtcttca caggcctgtg cgtcacggcc acagtgatgt atgacaaata caacccggac
     8581 gagttcatcg cgtcggtgga cgccgcgctg gaggcaggcg acgcccgcgc gctgaaaaat
     8641 ctgctcaagg gtgaggacct gacggtctcc gacgagggcg ccgccgcgct gtgccgtgct
     8701 ttcacagacg cggcgcagcg tgaagcgctg cgcggacagc tggaggacca ggtggtagac
     8761 ggcggcgcac agggggcgtt ccccgcgctg ggcgtggaaa aggaatcggt gttcttcggc
     8821 tacagccgct attctctcac cgtccacagc gtgcggctgc tgctcagctc cccggtgcag
     8881 aacctgcgtc tttcgctgga cggcgtgccc cgcacaggcg aacagacggc ggacggaatt
     8941 ttgtatcaga acctgttccc cggcctgtac acctgcacgg tgacgggcac caccgctgcc
     9001 gggcaggcgg tggagggcga cgcgacggac cttgcgctgc tgtcctccgt cgaacccact
     9061 gtgttcagcg gcgccctgcc catcgcggat attacggtgt ccggctgtgt gaacgacggc
     9121 gcggtcatca ccgtggacgg cgcggctgtg gagcaaaagc ctgtgaacgg cgttgtcacg
     9181 ctgccgcagg tggctgtggg cagcaccatc ggtatgcagt atacggctcc gtggggcgct
     9241 gtgacgacag cctccgtgca gttcgcagac aaaacggtca ccgcactggc gtttgaaaac
     9301 ccggtgacag agggcggcgt acccgcggcc ggggagctga acacgctgct caccgcccat
     9361 tatgccgcat atctggatgc gctgaacaat caggacacag cgctgatctc cggctgcacc
     9421 gaggaataca aagccgcgct cgcacagggc gttgtctccg acacacacaa ggccaacctg
     9481 tatgtgatgg gtaccgccga gtgcaatccc gccgccatca agagcaccgc ggcggacggc
     9541 actgcgcgcg tatcgtgcta tgtgaagctg acatacacgt attcagaccg ggaatcccac
     9601 gaggagacgc cggccacggc ataccgtgtg tacacgttca cctgggccga cggctggaaa
     9661 gtgagcgcct cggcggactc cacggaagaa gcctacaatg ccgcctcgat ggatgcgctg
     9721 ccctgaaaat cataaaccgg aggatacggc atgaagctct tgttcaaaca acgcatgttt
     9781 tcatggttcg acagctatga tatctacaac gaagcgggcg acgccgtcta cgaggtcaag
     9841 ggtgtgctga gctggggcca ctgcctgaaa atcttcggcc cggacggcgc ggagctgggc
     9901 acggtaaaag aagaggggtt ctctttcctg ccgcgttttg cgctttacgt gggcggcgaa
     9961 tgcgtgggac agatcaaaaa agaattcacg tttttcaagc ccgtgtttac gctggactgc
    10021 agcggctgga gcatcgaggg cgatctgtgg gaatggaatt accttgtcag caatgcggac
    10081 ggccgcacgg taatgaccgt gcagaaggaa ttactgaact ggacggacac ctacgtgctg
    10141 gacattgccg acagcgccga cgcactgtac tgcctgatgg tggtgctggc catagacgcc
    10201 gccaaatgct cgcagaacgg ttgaccgcgt gcatacaaaa ataaaaagtg ttttcgcggg
    10261 acctgattgc ggagtccgcc tatggtgt
//
LOCUS       DAWBSB010000068         9955 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1537_length_9955_cov_3.240808, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000068 DAWBSB010000000
VERSION     DAWBSB010000068.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 9955)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9955)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9955
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1537_length_9955_cov_3.240808"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3776..3785
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8860..8869
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1776 a         3334 c         2825 g         2000 t
ORIGIN      
        1 gtgcgcggcc gcctcggccg caagccgtac cgcgatgcgc ccgcgcagct cttcggccgc
       61 agcgcgggtg aaggtgacga tcagcagcct gtcggccgcg accggatgtg ccgcatcggt
      121 gatcagccgc accgcgcgct ccaccagcac cgctgttttg ccgctgcctg ccgccgccga
      181 gaccagaagc gtaccgcccc ggtcctcgat ggcggcgcgc tgggccggcg tccatttcgg
      241 cgcgctcata tatttccctc cccctgtgct ttgcacgcgt ccgcacgcgt ttcaaacaca
      301 tccttcggcg cctgcacgct tgtttcgccc cgcccgtcct cgtgcaggca gacgggacgg
      361 taatcgcata cgtcgcaggg acagtgggcc gccgtgcgaa ggggcaccgc gtctatttca
      421 cccgcgtaca ggccccgggc catctccacc accagcgcgt ccagatgctt ttcaatattg
      481 cccagctttt ccaggcttgc cagcttggcg ctggcgcggg gcgcaccgtc ctttccaaag
      541 gcgaacggta cgaacatgcc cgtggcgtcc cggtccatgc cgcgcaccac cagttcgtcg
      601 ttgagcacca gcccgtccac cttgtacacc ggggccgccg ctgcctcggc acgggagccg
      661 gttttcagca cagggtctcc ggccagatac agcacgcccg cggccacggg gtttttgtat
      721 acctgcgccg cgttgctgcg cagcgtgaac agatacagca gcatctgcgt gttcaggccg
      781 caatacacct cgtccagatt aaatgctttg tttcccgttt tgtaatccac cacgcgcaga
      841 taggtcctgc cctcccgctc cattacgtcc acacggtcta tcttgccctg cacctgcacg
      901 gtacggccgt ccggcgtgcg cagcgtcagc gggggaaccg cgccctcccc tgttccgatc
      961 tcctgctcaa aggcggccgg atggaacagg ctttgggcct gctccgcctg aagatatgcc
     1021 aacaaacgcg ttacgccgcg cttcagccgt tccaccagat aggcaaagcg ccgccctgcg
     1081 gcgggcatgt tttcggtcac gtattggtcg gccacctcgc cggccagccg ggccagctcc
     1141 tccggcgcaa gacgcgggaa ctccgcgccc gcgcggcgca tcacgtgctc caggatataa
     1201 tgcaccaggc tgccgctctc cagcggagaa agctccgcgc gtctgcgcgg acgaatgcgc
     1261 aggacatact gcagaaagta agaaaaccgg cagcggtaat attgttccac acgcgtgggt
     1321 gaaagcgtga gcctgtcgcc caaaagcgcc cccagagggc gcgtctcccg cgcgaaaaag
     1381 tgcgcgggcc gtgcggcgcg ctccatggcg accaagctct ccgccatcgt gtcctggcgg
     1441 cgcagagcgg cctccagcgc cgcggtctga ggcgtatcct cccggtaggc agctcccaac
     1501 aagtccagcg cggcggccgg tgtgggtgcg cgctgggctt cgtccagcac gtctgccgga
     1561 ggagccagtg cctccaccag cggctccatc gccgcgctca acggcgcgcc gcccgcctcg
     1621 ggagggacaa agctgacata caacgcctgc gccggcgccg tcaaagcgcg gtaaaagaac
     1681 atctgttcca gcagcgtgcg gttttcatag ctgccgggca tctccacgcc gccctgcacc
     1741 agcagatccc tgtccgcgtg ggagagcagg ccgctcgcgc ccagcagctt tggaaacttg
     1801 ccctcgctca cacccagcac aaagcatacg tccgggctgt ccagacgcat gcggtccgca
     1861 gtggtcacga tgaccgcgtc ctgcgtttcg ggaacatggc ccaggtccgc ttcgtgcagc
     1921 agcagcgcga acagctcggc gtattccgct gccgtcacct cgtccccgcc caaaagctgc
     1981 tccatctggc ccagcagatc catcatgttt tcccaagtgg cgtacagtgc gcgggcgcgc
     2041 agcgggtcgc ccgcctgctc aaacgcggcg gccgcctgtg cggtgtgctc cgcgccgctg
     2101 aaagcatcca gcagcagata cagctgcttg gagatgcccg ctgccgtctg tccccgcgcc
     2161 gcgtccagaa agcgttccac acggggcaca acttccgcgc gcagagcctc cgcccgggcc
     2221 agagccgccc tgtcctcatc cgtcatctgc gcgccgaagc ccgccggatt cttttcaaaa
     2281 ggggcgcgcc agtcggccgc tttcagctgc caggtgtaag cgtaattttc caaaaccgcg
     2341 atttcctcag gcgcaaaccc gcacaagtcg gttttgagca ggcgcaggag cgcctcggtg
     2401 gaaagccctt tcgacagcag cgacagcgcc gccgtaaaaa aagcggccgg cgcagtgtgc
     2461 tcggggctgg tggtctcgtc tgtgaaatag gggatgcctg ccagagcaaa ttcatattgc
     2521 aggggcgcgg cgtatgcgtc catctcgcgg cagatgaccg caacgccgcc gtagcgcagc
     2581 ccccgttcgc gcacaagcgc ggcgatgcgg caggctacgg ccttgcactc ggcgtaaaca
     2641 cctgcggcag gcgtgacaaa aaagcccgcg tgctccggcg cttccggcgc gccgaaagcc
     2701 aggcattggt tgaccgccgc aaggcccggg gcgttcttgt ggcgaaaatc ctcctgcatc
     2761 acatgcggcg cggcgatctc cacgctctgc ttgcctgcaa tgcgccgtag attctgcgcc
     2821 gtttttttca ccggactgaa caagccaagg cccgcttcgt tgtccgaaag cccgtcgcag
     2881 cacagcgtga cagtgcattc ctcagcctcc accagctttt ccagcatgcg gtattccggt
     2941 gcagtaaagc cgtcgaaatt gtcgataaaa acggctttgt ccgccaaaaa cgccgggtcc
     3001 agccggagcg cggcggcgga aatacggtcc gccgggtcca tggcggagcc ggcgatcaac
     3061 gcctcatagg cggcgaaaat caatcccagc tcgtgcagct ttccgccgtc ctcgcccgcg
     3121 gtgcgggcca cgtccagcag cgtctcgggg cttgcgcccg ccgttttcag ctctttgatg
     3181 gcgtccgcgc acatgctgca aaagcccgtg ctgcgccggt gacggcggta ggcctgcagc
     3241 tcgtcgccca gcgtatccat agcacggcgc actgcaacgg cccgggccgc gtccgtcagc
     3301 gttttcaccg ctgcgccgcc aaaggtcttg agcaccagct cagcaaagct ggtaaagctg
     3361 tatacctccg cacaggcgct gaacgcatcg cccaaagagc ggtacaccat cgtctcggca
     3421 gaggaagaaa actgttccgg caccagcagg atgctgcgcc gccgcgcggc ggcgcgcacc
     3481 ttcatctcgg ccagcacacg gcctgttttg cccgtgcctg aaatacccaa tatcagccgc
     3541 agcacaaagc tccaccccct tctgcagcaa gcgctgccat tgtttgctaa tccacaataa
     3601 aaaatagtta caggccattt ttggggccgt ctcccgcccc cggagccatc cacacaaaac
     3661 agccttcggc agtcttttat agtataccgc atttggccgg ccaaaaaaag ggcatgcagg
     3721 catttggcgg aatggtacag aaaagcggcg gcaggaaaaa tcctgccgcc gcctgnnnnn
     3781 nnnnnccgcc tgcgggatat gtgggctttt gaagccgctg taaggcttag tagttctcgg
     3841 ccttgagctg gaaatatgcc ttcggatgcg cgcagacggg gcacacctcg ggagcattct
     3901 tgccgatgac gatatggccg cagttggagc actgccagac catgtcgccg tcgcgggaga
     3961 acacaaggcc gccctcaaca ttcgccagca gcttgcggta gcggttttcg tgctcacgct
     4021 cgatctttcc cacttcttca aacaggaaag ccaggtggtc aaagccctct tccttggcgt
     4081 ccttcgccat ctgggcatac atatcggtcc actcgtaatt ctcgccctcg gcggcgtcca
     4141 gcaggttgtc ggccgtgccg ggaatggcgt cgccgtgcag cgccttgaac cacagctcgg
     4201 cgtgctcctt ctcattggca gcggtctctt caaagatctt tgcaatctga acataaccgt
     4261 ctttgcgcgc cttggatgca tagaacgtgt acttgttgcg ggcctggctc tcaccggcaa
     4321 acgcggtcca cagatttttt tcggtttttg ttcctttcag ttccatctta ttacgctcct
     4381 tcttctatca tgtgattttt atgtacagcc gcagcgcggc ttccggcaaa atcagcgcgg
     4441 acttacacct tgcccgcttt ggcacactcc gggcacaagc cgcggaacac aatgtcgtga
     4501 gaaacaccgg agtacccggt ctggcgtgcc acggcctcgc gcagcgctcc cggacgcggg
     4561 tgcgtctgcg aaacagccgc actgggtgca tatgatgtga tggtgttcca aaagcatatg
     4621 gtcgaaccgg tctgcgccgc cgggcatctt gatatgccgc agcaggccat cctcagacag
     4681 gctgccgagg ttgcggtata ccgtcgcctt gcttaccgtg ggatggctgc gcgccacttc
     4741 ggcatagacc tcctccgctg tgggatggtt ggcaagacga aacacggcgt ccagcacaag
     4801 ctgcttctgg atggtgttgc gtttttccat aatgggctcc ttacttactc cctcgttcaa
     4861 gggaaggata tggttcttta ttgatatttt atactattta gcacaaaatg tcaagcaaaa
     4921 ctgcaaattt attttccgcg cccgcttctc atccggcacg cacaggcgca cggaagtaat
     4981 ccacctggaa cgtcacgcag aagcccagct tttggtagat atgataagcg ggtgcgttgc
     5041 cggaatccac gtccagcact acgccgtccc gcatggcccg ggcctccgcc agcgcaagga
     5101 gcatcagctc ctgccccagc ccctggccgc gccgtccggg cgaaatgcct acaccataca
     5161 aaaacgcccg gccgggatgc tcccccaggt tgatgtgaaa catgcccacg ggcgcgccgt
     5221 ccagcatggc cagataagcc gcgcgctccg ggttttccac cgatgccgcc acatagttgt
     5281 ccacatccgc ttcacccata tcattttcca gaaacaggcg gtagggttcc gcgccgtccg
     5341 cgtccacacg ttgtagtgcc aaccgtcctg caggcaccgg cgcaggacct gtatattcca
     5401 tccggtattc cgaatggctc catacacagc cgggcgttgt ctccagcacg gcgcgccccc
     5461 ctgcggagcg cggctccacc gcaaacaaaa attccggaat gcccgctgcc cggcaccgtg
     5521 cggcggcacg ggcgcacagc gccttgaagt atcccctgcg ccggtgggcc ggatgggtaa
     5581 aagccaccac ctccgcctgt tcccgcgtgg gcagaaacaa tgtcaaaaac gcggcgggtg
     5641 catcctccgg gccgggcgcg tcctgcggcc acgccagata aaagcatggc agcgcgcggt
     5701 caaagttcag ctcgttggac agccatgccc cgttctccaa cgcttcgggt gcgcaggcgg
     5761 cctgctccag cgcgcgcacc tgttccgtct gacgcggcgt cagcacggca gtttcgaata
     5821 tgtacaaacg catcctcctt ttccgccgtc ccccaagggg cgtcaaataa aaaagcgccc
     5881 gccccggaag gtctccagca ggcgggcgct gcacagcgca ttattcgggc ttctgaggct
     5941 gggcggcggg acgtaccgta gcttttttac cgctttggcg cagcgtggag cgcacggtct
     6001 ttacctccgt catactcttg gcaagctgct cctcggtatg gcgcagcatg ttttcgcagt
     6061 aattggtaac cgtggtgcgc atctcgcggc tctggctctg ggcgctggtc agaatctctg
     6121 tagccttctg ctgggcggcc tttacgatgg cctcctgggc caccatggcg cgggcgcggt
     6181 cctccgcgcg cttgacaatg gcttcagctt ccttcttcgc gctttccacg atatccgcac
     6241 ggtcctgtac aatggccttg gcctggcgga tctccgcagg catgttcaag cggatatcgt
     6301 cgatgatatc gcgcaccttc tccacatcca ccatgcgctt gccgccggtg aagggcagat
     6361 tgacggcctc ctccagcgtt tcatccatta catccagcaa ttcgtcgatg ttcataatgg
     6421 tcactccttt gctcgcccct gtgtgaggcg ttctacgatg tctttacaaa tgccgtccgg
     6481 caggaaatcg gtgacgtcgc cgccaaagct ggccacctga cgcaccatac tggacgaaag
     6541 gtacatatat tccggatcgg tgatgacgaa catggtctcc agctccgggt tgagcttttt
     6601 gttggtgagc gcctgctgga attcaaactc aaaatccgtt acggcgcgca ggcccttgat
     6661 gatggtgcaa gcgccgacat tgtgggcata ttcggccagc aggccggtgt agacgtccac
     6721 tttcacattc gggatatgcc ctgtgacgcg ccgcagaaaa tccgcgcgct cctgcgccgt
     6781 gaaggacggc gttttattgg ggttcaccat caccagcacc gtcaccgcgt caaacagttt
     6841 tgcgctgcgc tcgatgatat ttaaatggcc tttggtaacg gggtcgaagc tgccggggca
     6901 gatggcgatg cgcatgcgca cgccctcctt ttctgtgaat tgcagagcgc tcagaactcc
     6961 gcgtcggcct cgtagcgcgc cagcgccacg gtgccgtatt tgtattgttt tttcagcgtc
     7021 agcgtgccgc agcgctcggg caggggcgcg ccgtgctccg tttccgccag caccgtcccg
     7081 cccggggccg tcacggccgc cacagcaggc agcagcgcgg ctacggtacc ctgacggtac
     7141 ggcgggtcca gcagcacaag atcgaaccgt tcccggcatg cggccagata catctctgcg
     7201 gccgtttccg ccacgctggc tttttcaaac aggccggcgg ccttcaggtt ttgccgcacc
     7261 accgcacagg cctcccggct gctgtcgata aacacacagc gcgcagcgcc gcggctgagc
     7321 gcctcgatgc caagctggcc cgaacccgca aacaggtcca gcacacgggc gcccgccaaa
     7381 aggaactgga cggcgctgaa catgccctcc ttgacacggt ccaccgtggg ccgtgtgatc
     7441 tcttcaccgg gcagcgcggc cagccttgtg ccgcgcgcgg taccggaaat aacgcgcatc
     7501 tcattctcct tatacgcacg ggcggccttg ccgccgcacc gcacatcctt tctttccgcc
     7561 ttttgcggcc gctgcgcggg caagtgcggg acaagccttg atttctttac tcattatcgc
     7621 cgtttgcagg cgttttgtca acttcctgcc ggttacagaa ttgctctttt ccaacgtgcc
     7681 gctccgtgct tctttacgcc tcggcggaag gctgctccgg ctgcgggtga aaatgggcat
     7741 acacggcccg cgccgccgcc ctgcttacgc ctttggcccc ggcaagctgt tcctcgctgg
     7801 cctccttaac ggcagacacc gttttgaaat gggccatcag cgcgcggctg gtggccgggc
     7861 cgacgcccgg cacgctttgc agcgtggagg aataggcgcg gcttttctgc atgcgcctgc
     7921 ggtaatcgtt ggcccagcgg tgcgtctcgt cctgaatgga cgtcacaaac gtgaatacgc
     7981 ccctgtgcat ggccagcatg atctctccgc ctgcggcgct cacaaggccc cgcgtgcggt
     8041 gcttgtcgtc cttcaccatg ccgaacatgg gaacatctgc aagctgtgtg cccgccagcg
     8101 cggcctgcac ggcgcttacc tgccctcgcc cgccgtcgat gagcagcagg tccggcttta
     8161 tgccgaactg cccttttgcg cccttttcgt attcggcggc gcggcgcgcc agcgtttcgg
     8221 ccatggaagc ataatcgtcc gtacccgcca cggtgttcat cttgaagcgg cggtagcccg
     8281 cttttttggg ttttccgtcc tcaaacacca ccatacccgc tacgcttgtg ccgtcgcccc
     8341 agttggagat gtcgtagctc tcgatgatcc tgggcggctc tttcaggccc agcagcgcgg
     8401 ccaattcgtc cagatgtttc tgttcgcggg catagcgtcc gctctcccgt gccaaacgtt
     8461 ccacggcatt ggtgcgcgcc atctccacca gacgggcggt atcgccccgc tgaggcacat
     8521 acagccgcac cttgctgccc cgcgtctcgc ccagcaggcg cgcaagctcg cccgaacccg
     8581 cgggcagctc atccacggca atgctcttcg ggatgaactc gttgtcctcc agataatagc
     8641 ggggcagaaa ctcctcccgc agggcgggga tgtcttgggt gtcgtgaaac aaaaactccc
     8701 ttttgtccac cagacgtccc tctcggaaac gcagaatggc tgcgcataca gccgacgcgg
     8761 tgcccgcgaa ggccaccaca tcctgctcgt ccacctcgct gcgcaccacc ttctggcccc
     8821 ggctcacctt ttcaatggct gcgatctaat cccggatgan nnnnnnnnnt tcgaaatcca
     8881 gccgttcggc tgcatcggcc atgcgctcgc gcagcagctt gaggatctcc ccctggccgc
     8941 gccgtatcat atgcacggca ctctgcacag cttcattgta gtcctcccgg gagatttttc
     9001 cgctgcacac ggccatgcac cggccgatgt gcgcgttcag gcagggacgg cctttgccga
     9061 tatcctgggg gaattttttg ctgcagcgcg gcaggcggaa cacattgcat gcggtctcca
     9121 ccatttcgcg cacggcaaag ctggaggtat acgggccgat gtaatcggca ttgtcgtcgt
     9181 ccttttgcag cgcggccgaa atgcgcgggt atgcttccct tgtcaccttc acatagctgt
     9241 atcccttgtc gtccttcaat aaaatattgt acttgggctt atgctgcttg atctggctgc
     9301 attccaacac cagtgcctcg aactcgctct gcgtgacgat cacatcgaac tcaaaagcgt
     9361 tctggatcat tttcgtcacc ttggcgtcat gcggcacgcc ctcgcggaaa tactgcgaga
     9421 cacgcgtgcg cagacgcttg gccttgccga tatagatgat ctcgccggtc ttgtcgcgga
     9481 tgatatacac tcccggcaaa agcggcagca tttttgcttt ttcccaaagc tcacgcttcg
     9541 tcatgcgcgg cctccttttt tacattgctg acaaatactc tgcggatagc ttgccccgtg
     9601 cgccgcaaaa aaatatgcgc cgcccaacgg gcggcgcacc tcacgcaagg aaatttattc
     9661 gctgaaacca gacttcaccg gcttgatccg tcggccttgc gccgcgcgcg aaaccgctta
     9721 cgcgcacggc gcgcaacgat tccgaatgaa aagggaatcc gcccttttag aggcgttcgt
     9781 cgtgcggcct gcgctttgct gcacaaaact gcatgacttc gccgtcggct ttccccgttt
     9841 cacttattca ctgaacccgg aattcaccag tttgatcagg ccttcgacag cggcctgctc
     9901 gtccgcgccg tcagcgatga tgcgaatcgt cgtaccgccc acgatcccca gggac
//
LOCUS       DAWBSB010000069         9854 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1551_length_9854_cov_2.942953, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000069 DAWBSB010000000
VERSION     DAWBSB010000069.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 9854)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9854)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9854
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1551_length_9854_cov_2.942953"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6797..6806
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7962..7971
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2038 a         2807 c         2933 g         2056 t
ORIGIN      
        1 cttgatgcct taccgtagcg gcggattcgg gactcccatt ataggagaga atgggaaaat
       61 cgttgcacat gcttctttac atgtcatttc tagccaagcg ctgtttttga actgctttac
      121 tgcaatgtcc attgcgtctg gacaatattt tcttgctcaa attcatagcg agctcaagat
      181 gatgagaatg aatttggaca aaattttgga gttcctgtac ggagacaaaa aggcagaact
      241 gatggccgaa ataagttttg ttaaatacgc ctatcaaaac tataattcta tcatgtcgca
      301 tgaatcgcaa aggacggcaa cgatagccag tattcaagag gctaagaaag tcgcgatgaa
      361 agatatcgaa ttctatatga acgaccttga tttggcggca aaaaccaagg ataattcaga
      421 gctaatatcc actgtcaata aggcgtttca aatcaaagaa agcctggagc tttcgatgca
      481 actgtatgtg ttcagcagct tattggaatt gtattattct cagaatacgg attcaagtta
      541 tttaaaatat gttgaagctg acgtgtcctc atatatcgat aaatgtgaga agcggatgct
      601 aagcagcttc agcgtccttg acatatttgt ccgagattac aaaggaaatc ttcttaagaa
      661 aattgatacg tccgaaatag agaaggaggt tagtggatta ctcgatcgtc tgaacaacgg
      721 ggaagagatt ccgatgcggc aggttttgca cgcagcaata ggtgcacaaa cgaagaagac
      781 agaatattat atggcaacag atggaaatat ttatttaaag accgcgtgaa ttttacattt
      841 ctatgaaaag acagcattgc tttcgagctt ttctgtcttt tctttttgct gatttatagc
      901 ttcgcgcaaa aaacacgggc ccctatgaag aaaaaagtaa aaaaacatga gtggctatac
      961 aaaaatacat aaggcaatag aacagcagcc cgggaagccg ccgcgttgag cggcgcttcc
     1021 cgggctgctt tatctgtgcc ggataaactt tttgtgcagg tcacacttcc ggaaaatccg
     1081 ggccgaaatc gtccatcaaa acctcgtccg cgtacaaagc gggaaaccag gcgttttctg
     1141 caaggttttc atacacgttt tcgctggtgg cagtttggcc gtcccttcgg gcctgctcca
     1201 catggccgtg ggccttgtgc tgcttgccgt gggcgctggg ctgcccgtgc cggttttggc
     1261 tttccttgtt ggtttggttt tgcattttaa acacccccgt gctgttattg tgcgcagggg
     1321 ggcgcgggat acgcaaggca atttcttccg gaaagcgcgt ccctttgccg ggcgcggcgc
     1381 gggtcaatcg tcgccgccgt cctcctcttc ggcggcttcg ccgctttccc cgccgtccgc
     1441 gctgccgtcc ggcgcgtctg cccctgcggc ctcctccgcc tgggcggcca ggcgcgcggc
     1501 ttcggccttc gcggcctcgg cctcaatgtc gatgtcctcg tccagataca gcgcccgccg
     1561 tttggcgtgc agcttttcca gctcccgggc ctgttcttcc ttgatttttt cggcctgctt
     1621 tttgtaggcg cgcacggcgc ggcgcgcgct gaccacgcag acgccgatca tcacggcggc
     1681 catggccaga tacagccagg aaagccatgt ccaggacgca aagggaacgg aaaaggccgt
     1741 caggccgcat tgcagggcca tatacgcggc cgcgatggtg aggatcaccg ataaaaacat
     1801 gtttttcacc tcattgattt tttgtgcggc cgcacagggc cgcaaattac aacatatccc
     1861 attatagccg ggatttgtga acaagaaaag cccccctttt gccttttgaa caggtttagg
     1921 ctataaataa cctccctttg ccgaaattga cggaggggca gattcatgga cggcaagccc
     1981 ggcaaaccgg aattagttat ttttatcctc tccccggatt ttcttgtttt tccgttcttc
     2041 caactccgca atttgtttca tcaaacaatc tgaaaaagcc ccttcaaccg gtgcagcttc
     2101 tcctgttgta tagcagtcca gaaggactat gttattatcc ttgtcaaaac aaaatatttc
     2161 atcaccattc cctataatgg acaagaacgg gataaaacca gtaaaccctt catcgtgcag
     2221 ttctttcgtt ttttcacgaa tatccaaaaa atcgggtatt ccattggcaa ttccatagac
     2281 tataattcct cggcagaacg accaaaatgg gcctatgtca tactgtttcg cccgtggcca
     2341 tatttcttcc cgaacctcca tataaagtcc gcctaatggg gacatagtga actctctaaa
     2401 atcagcagga agatttatcc catactgatg ttcaaaattc tttatatcgt gctccgacgg
     2461 ctcatttccc atacaagcta caacctgata agtctgctta tcataattat ggaaataatc
     2521 ataaactttt tccaatgcca tcgtatgcct cctgcaacta ccaacttttc tttttattta
     2581 cctatataca atattttccc gctttattca atgcatcgtg aaatggacga agctgaagcc
     2641 atccgtccaa aggggaatcg ggagaagaaa agtcccgcat tgcatttttt ccgctgcgcc
     2701 cttatactga aagcaggaat actgcacggg cgcggcgcgc ccgtgggaaa cggaaaaggg
     2761 gaatcgttat ggaataccgt tcttacaggg atacggactg ccgcccttcg ctgctggggc
     2821 tgggctgtat gcgcctgccc acgctggagc cgggcggcac cgccatcgac tacgcgcgcg
     2881 ccggggcgct ggtggacgag gccgtgcgcg ggggcattac ctattttgac gtggcgtatc
     2941 cgtaccacgg cggcggcgcg gaaaaattca tcggcgcggc gctggaaaaa tatccgcggg
     3001 aatcgtttta cctggcaacg aagatgccgc tgtggaagct ggaaacgcag gaggatatgg
     3061 agcaggtgtt caacgagcag ctggccaact gccgtgtgga ctattttgat ttttacctgt
     3121 gccacgcggt ggacgccgaa cggttcgaga agatccggcg catcggcgcg tttgaatttc
     3181 tggcgcggaa aaaggccgag gggaagatcc gccgcctggg cttctcgttc cacgacaagc
     3241 ccgccgtgct gcgcgcgata tgcgccgcct acccgtggga ttttgcccag ctgcagctga
     3301 attatctgga ctggaccgtg cagcgcgccc gggagcagta cgaaatattg caggaatacg
     3361 gcctgccgtg catcgtgatg gagcccgtgc gcggcggcgc gctggcttcc ctgtgcccgg
     3421 aggccgaggc ggtgctgaag gcgaaggcgc ccggccgcag cattgcgtcc tgggccatcc
     3481 gcttcgcggc cagcctgccg ggcgtgctgg tggtgctttc gggcatgagc agcgaggaac
     3541 agcttgcgga caaccttgcc gcgatgtcgc ccttccagcc gctgacaaag tccgagcaga
     3601 acgcgctcac ccgggccgtg cgcatcgaca agcgggccca gcgcatcccc tgcacgggct
     3661 gccggtactg catgccctgt ccgttcgggg tggacattcc cggcctgttc aaagcgtaca
     3721 accgctgcgc cgcggcgaaa agcaccggcc gctttttgaa agagagcggg gatatccccg
     3781 ccgccgcccg cccggccgcc tgccgcgcct gcggcaagtg cgcccgggtg tgcccgcagc
     3841 acatcggcat cccggaggag atggcaaagc tgcgcgcgct ggaggaaaag ctgcgggaaa
     3901 aataattccc gcagggcaca aaaagcctca tgcagatgaa ctgcatgagg cttttttcgg
     3961 gcttgccgag caaagcggca aaaacggccg ggcggggccg tttttgcccg caggcttaaa
     4021 aggacgggcg ggcgcccggc cgcgccggca aaacagcgcc gcggccgctg ctttggcggg
     4081 ggtcaggggc ggctttcccg ccggagcgtg gtgggcgtgc ggttctccag ctttttgaac
     4141 acccggatga agttgatgtc gctggaaaag cccacggtct gagcgatgtc cttgatgttc
     4201 atatcggtgt tcaggatcag gctcttggcc tggtccaccc gcaggcggtt gatgtactgg
     4261 atgatgctga cgccgtaggt ggccttgaac gtggtggaca gataggagtt gctgacgttg
     4321 agctgctcgg agacgagata aaggcccatc atcgggtctg caaaattttc gtcgatgatg
     4381 cgcttggcgc gcacggccac gggctccttg tcctcggcgc ccggcgcggc ggcctgtacc
     4441 agaacggcca gcgcgtgctg ggcctggcgg cgctgggcgt ggggcgggcc ggcggccgtc
     4501 agcgcgcgca cctcctgcgc ggcgcgggcc tcgggcaaag cggcgcgcag cgcgtccgcc
     4561 agcagattgt ccacggcgct ctgccgcatg gcctcggtgc cggcgggcgc gcccgcgtac
     4621 aggtattcgg tataaaggcg ctccatcagc tgctgggcct gggcaaactg cttttcgtaa
     4681 atgcggtggc tgaacgcctg catcacgccg gggtcgccgc agtccgcgcg ggtaaggcgg
     4741 ggcgtatagc ggttcaccgg gtggcgggcc gagggcgcct cgcagcgcag cgccagcagc
     4801 gcgcagttgt agctggtcag gatgtccgtc atgctgtcgt agcaggggcc gacgcccgcg
     4861 tgggcgggcc gggcgggaaa ggcctggtcc agcagcgcgc gggcaagggc ctcggcctgg
     4921 ctgtcgggca ggcattcctc cgcgttcagc agcaccacgt agcggccgcc gtaagcggat
     4981 accagcccct cgtgccccag cgcggccagc gtgccgtgca gcaggccggg ctccggcccg
     5041 gcgtccggga cgctgcgctc ggtgccggcc agcaccagca cctgataggt gggatgctcg
     5101 aagctcacct cgtagcgctt ggccgcggcg aagatcgcgt tttcgctgtg caggccgccg
     5161 cgcaggacgg tgctcaggaa caggccgttc agcagcgtct ggtgctcctg catcagctct
     5221 tcgctcttgt atttttccgt catcatctgt tcgatcttgt tggtgatgaa ctggtattcg
     5281 tcctgcgcgc cggagccctg cgcccccagc gcggccagca gcttttccat aggcttcgcg
     5341 ttgcggcggc tgatgaacac gctggccgcc acgcccgcgg ccaggcagca caccgcgccc
     5401 gccacacaga cggccagcgt aaagcgcacc gtgcgcagca ggccgcgcgc ggaatacacg
     5461 ctcacataat ccagcccggg cagggcggag gcattgaaaa acgcaaagct gccgccgctt
     5521 tcggggctgg cctgcgggtc cgcgcaccag gccgcataaa gcccgggcgt gtcctccagc
     5581 agcgccatat tgcctgtggc ggccaccagc tgcccgtcca gcagcacccc tgtggcagag
     5641 tctccgggca ggcctgccgc gtcgaaggcg cgcagcagct cctcccggtc tatctcgatc
     5701 acaacgaccg ccgcgatctc gcccgtcatg cgcatctcgc gcacatagca caggcgttcg
     5761 tccttgggca tctgcagcag catgaagcgc gtgtcatggc ttttcgtcag ctccgcgagc
     5821 catgcgtcat agccctcgcg ctgcggcacg ccctgcaggg cgtaatagga ggcggcctcg
     5881 aagcagccca tattgcccac cacaagcccc gtgcgcgggt aatagaggta tacgccccgg
     5941 gcgagcttgt tcgtgacgag aaaatcccgc agcgtgacgg aaagctggta cgcctgcgcg
     6001 ggcagggtgc cgggcagctc ccgcaggttt ttgagcagcg tgttggcgtg cgtgatctca
     6061 atggtagtgg aatagcggtg cagctcctcc aggcgggtat cgacgctttt ctgcacataa
     6121 gccgtcaggg cggtgttggt ctccaccacc gtgcgctcca gcacatgcag gacatacagg
     6181 cacagcagcg agcagagcag caggcacgaa agcactacgg ccagaatgga gcgcagccag
     6241 cgcatggtat gttttttgcg gtgcggcaca aaacgcgccc ccttttccgt ttcctccggt
     6301 atccggatag gactattcta tcataaaacc gtgccggagt agaaattgat tttttttggc
     6361 ccctttttcc catcgggagg ccgaaattgg aagatttgtt aatccaaagc ccgttccatc
     6421 taaaaaaatg taagtgaaag acaaataagg tttggtcaat gtgcataaaa cgaacggtga
     6481 aaaatagacg ctggttattc atataggaaa aattgtaagt ttacagcaag atgggctttc
     6541 ggctaccatg aaagcatgga aggggagcgc ggaggacgtt cccggcggga caccgccttc
     6601 cacatcaagt aaaacaggag gaaagaacta tgaaaaaact gctcagcatc cttttggcgc
     6661 ttgtactggc cacgggcctg ttcgcagcgt gctcgggctc cggctccggc tcgcaggcgg
     6721 cgggaagcac ggcctccggc agcggcgcgg ccgccgcgcc ggacgggaaa aagacagacc
     6781 tgctgctttg gctgccnnnn nnnnnnggcg acgacggcgc gctggacaag gagttctgga
     6841 cggaaacgct ggcgccctgg gccgcggaaa acaacgtgga cctcaccatc gagatcacgc
     6901 cctggggcaa ttacgaggaa aaatacctga cgggtttttc ctcgggcgaa ggcccggacg
     6961 tgggctatat gtatctggag atgttcaacg attttattga gatgggcgct ctggagcccc
     7021 tggacgccta cataacggat gcggaccggg agaattacct ctacctggac aagggcttta
     7081 tcaagggcaa gcaatacacg atgccgttca tcgtgggcaa cgcgcgcatc ctgtatttca
     7141 atatggacat tctggagcag gcgggcgtga cggagctgcc cgccacctgg caggacctgg
     7201 tggacgttgc cgtaaagata aaagaggcgg gcctgcccgg cgtgatgccg tttgcgggcg
     7261 aatgggccga cccggccatc ggcgcgctga acaacctgta ctatccgtac ctgtggcagg
     7321 cgggcggcga catttacaac gaggacggca ccaaggtcgc cctgatggac aacgacgccg
     7381 cggtgaaggc cgcccggttc ctgtacgacc tcaagttcaa atacggcgtg ctgccggagg
     7441 aatccatggc gctggtgggc accgaggtgc gcaaccagtt cattgagggc aatatcgcca
     7501 tcgcttccat ggacgcgaaa tccggcacgg tgctcacgga cgcaggcgtc aactgggatt
     7561 tcatccccag cctggaggac gagacccgcg ccacatggat cgcctctgac gcgctcatca
     7621 tgaacagcgc gtcccagaac aaggagctgg ccgccagcct catcaaatac atcaccagcg
     7681 ccgaggtcat ggcgaaattc cacaccgaga tcgctccctt cccgcccatc accagggacg
     7741 aggcgtacaa cgacgacgag cgcttcaaag agatgtacga ggacgccgag cacctgcaca
     7801 cgctgcccgt cgccaacggc gcgttcaagg tgatggacac cctgtataag aatctgcagc
     7861 tcatgatgct gggcgacctg tcccccgagg aggccatcca gaacaccgtg gattacgccg
     7921 agagcatcgg ctgacggcgg aggacgggcg ggcgggcgcg cnnnnnnnnn ngcggtagga
     7981 agctgaaatc cggcgggcag gcgcggcgcg cctgcccgcc gcctggataa aaggggttgt
     8041 gtgttatgat caatcgcaga aaggaggctg cgcagggctt tttgtatatc ctgcccagcc
     8101 tcgtgctgat cctggtattc tcggtggtgc ccatcctgat gagcctgtac ttcagcttta
     8161 cgaagtacaa tgtgctgcag ccgccccagt ggacggggct ggacaattac atccgcctgt
     8221 tccgggaccc ttatgtgcgc gcttcgctga aaaacacggt ggtctttacg gtgatcaccg
     8281 tgccgctgca gacagccggc tcgctggtgc tggcggcggt catcgccgag ctgttccaca
     8341 acaaattcgg caattttgta aaaagctcgc tgttcgtgcc cgtcattgcg tcggccatcc
     8401 ttgtgggcac gctgtggttc acgctgctgt cgccgcgcgg cgtggtgaac agcatcatcc
     8461 atgcgttcgg cctgccctcg gtgaactggc tgggcggcaa gctcacgtcc atgctcagcg
     8521 tctgcatcgt cagcgtgtgg aaaaacgtgg ggtacttcct cgttatttac ttcgcgggca
     8581 tcatggatat cccgcgcagc ctgtacgagg cggccgaggt ggacggcgcc aatgcgttcc
     8641 agcgcttttt caagatcacg ctgccggggc tttccagcgt gacgttcctt gtggtgacgc
     8701 tggggaccat ctggtcgttc caggtgttcg acctggtgta caccatgacg ggcggcggcc
     8761 cgggcacctc caccgtgacg ctggtgctca caacctacaa cgcggcgttc aaagagtaca
     8821 atatgggcta cgcaagcgcc gtggccatgc tgatgttcgt gttcgtgctc gccgtttcgg
     8881 gcctgcagaa gctgctgctg aagggcggtg aggactgatg agccgcagag cgaaaaaaaa
     8941 cgccgcgcgc gcggtgctgg gggccgtgct gctggcaatg gcgctgtttg cgctgctgcc
     9001 ctttgtgtac atgggcctgg tttccctcac gcagaaaacc gtgctggacc tccggttcga
     9061 tcccgcggag ttcagcttca aaaactatgc ccgcgtgttc agcaacttca acatcgggcg
     9121 caacctgctg aacagcgtca tcgtcacagg cggcgcgtgc gtgctcaact gcatcgtgtg
     9181 cgccatggcc gcctacggct ttgcaaaaaa gcattttccg gggcgcgacc agctgttcat
     9241 ggtctatctt gccacattga tgatacccgg gcaggtgacg ctcatcccgg tgttcaccat
     9301 catcaaaaac ctggggctga tgaacaccca catcgcgctg atgctgccca tcctgaacgc
     9361 gttcggcgtg tttctggtgc ggcagttcat ggtcaacgtg ccggacgagc tcatcgaggc
     9421 cgcccgcatt gacggctgcg gtgaaaaccg gctgttcctc tccgtggtgg tgcctttggt
     9481 gaagccggtg ctcgtctcgc tcaccatctt tacgttcatc acctgctgga acgacttttt
     9541 gtggcccctt gtcaccgtga cggacgagcg catgcagacg ctgacgctgg ccattgcggc
     9601 gctcaagggc aactactcca ccaattacgg cctggtgatg gccggctcca cgctggcgtt
     9661 cctgccgccg ttccttttgt atgtcgcgct gcagaaagaa tttgtggaag ggattgcgct
     9721 ttccgggatc aaagggtaat atgaaagaag tcagactgtc gaaaaaggca aagaaggaaa
     9781 agaagcgtcc ggggaaaagg ctggcggtgg caaagccatg catctttgcg cagcaaagtg
     9841 caagccacgc ggcg
//
LOCUS       DAWBSB010000070         9794 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1560_length_9794_cov_2.895266, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000070 DAWBSB010000000
VERSION     DAWBSB010000070.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 9794)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9794)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9794
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1560_length_9794_cov_2.895266"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5980..5989
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9541..9550
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2070 a         2789 c         3049 g         1866 t
ORIGIN      
        1 gtcccagcgc ttttatcgcc gggcagctgg agagccatgg ctacggcgcg aagctgtggg
       61 tcatctccaa cggtgttgcg gacgcgtttc gtcccgcgca ggtgccgcgc gcaccggagc
      121 tggagggaag gttcacgatt ttgatgatcg gccgcctttc cggcgaaaaa cggcaggatc
      181 tgatcataga ggctgcgaaa cgcagcaaat atgcagacag gctccagctg gtatttgcgg
      241 gcaagggccc taaggaaaag gcataccgca agcttgccgc ggagctggcg catccgcccg
      301 tgttcggctt ctacggccag gacgagctgc gccggctcat caatatgtgc gacctttacg
      361 tccatgcctc cgacgccgag atcgagggta tcagctgtat ggaggcgctg gcctgcgggc
      421 tggtgccggt catctcggat tcgccgcttt ccgccaccgg ccagttcgcg ctgtgcgcgg
      481 agagcctgtt ccgtgcgggc gacgcggatg atcttgcgcg caggatcgat tactgggtgg
      541 agcatccgga ggaaaaacgc gcttatgccg aacagtacgc gctccggcag gatgagaatc
      601 gtgtggaagc ctgtgtggcc cgcgccgagg agatgtacgc ctcggcgatc cgggacaagc
      661 ggcgcaaagg ctaccgcaag gtgcccctca gccgctggcg gcgctgcaca agccccagct
      721 gcgaggccat ccggcgcaat ttctgtcagg gcggtacagt gcgcacgctg ttgttctggc
      781 tgttcaccac gctgctttct cccatcctgt ggctgctgga ccgcctttgg ctgggcgcca
      841 ggatcgaagg ccgggaaaat ctcgacgccg tgcagggcgg tgcggtcagc ataatgaacc
      901 atgttcatcc gctggattgc accatggcaa aggtggcgct gtttccctac aggctgtggt
      961 ttatcagttt ggcgtccaac ctgcaaaagc catttacagg ctggctcatc cgcttctgcg
     1021 gaggcgtgcc gctgccggat gatatccacg gaatggcggc gctggagcgc ggcatggaag
     1081 cgcgtatccg cggcggcgct ttcgtgcatt tttacccgga gggcatgctg gtgccgtacc
     1141 atgaaggcct gcgggcgttc catccgggtg cgtttaccac cgcggtgcgt gcaggctgtc
     1201 cggtggtgcc catgatgctg tgccggcgtc cggcccgtgg cctttgggcg tggcgcaaaa
     1261 agccctgttt tacgctgcgc atcggcgcgc cgctgtacgc ggatgcatcg ctgccgaaaa
     1321 aacaggccgc gcgggatctg cagctgcggg ccgaagcagc tatgcttgct ttggaacgcg
     1381 gcgaaacgcc tcctttggcc gtaggggaac ctgcgttgga cgcggaatga agtgaagcgg
     1441 ccggatggcg gtttcgggca tgcgggctgt cccggggctg tagacatcag gaaaaggttt
     1501 tatttgcagc gctggaaaat caagcgtccg gctgctttgg cggtgttccg tcaaagcagc
     1561 cggacgcttt tcgttttttg tttttacagt ggggcttacg agagccttgc acctcccgga
     1621 tacagagcgg acgcatccgg cggcgcttcg ctgagcacga ctgcacggcg ttccatttcc
     1681 gcgtagcgca gcaggaaggt gccgcattcg atctgcagcg cgccgtataa cggatcgcgg
     1741 atataaaaat agtccgcgtc gtagccgcat accacaagac agtgcaggtt tttgtaggga
     1801 tggtagacgg agctgtctga atacagccgc caggaataat cgctgtacac caagggctca
     1861 aagccgatgg tcgcccagac caccaccggc cgtccctgag cgatgctcct tcggagcacg
     1921 taaccgtccg cgccgctgat atccaccgcg cgcagcgtgc tgtcctgttc cgcaagataa
     1981 cggtttgctc cttcggccac ggcaggggcc aggcagtaaa agccgaacag cgccggatcg
     2041 cctgcgtagg cggaggcagg gtccggcccg taggtggaaa accaggtgta ggtgaagtcc
     2101 gagcggggga tataatcgta tgcaacggag agcttatcgg cggcaaatcc ataatattgc
     2161 agcagcattg tcagcgcggt ggcttcgcag ccgttgggca tttcgggcca ctggtaaaca
     2221 acggggacgt ccagcagaac ggcctccggc agaggcggac ccagagattc tttccaggcg
     2281 cgcaatgctc ctgcgaagga atatcccgtc gcggcataag cggcggcggg cagggggcgg
     2341 ggacgaaacc cggcgccgaa cagcacggca caggccagta tgagcagaat ggattttatt
     2401 ccggcgcgca tgccgcgcac cctcctttcc gtggccgtcc gtccgccgca gtgcggcgcg
     2461 ggggccttcc ttttaacata atacaaaaaa cggcgacaca caagcaagcg cgggggattt
     2521 ccacattttt tcacagatct tcagtattca cgggcacaga aaagccccgc ccgaaatgcg
     2581 cgggacgggg ctgcggcatt ttgattgaaa acggcggcgc tcattcggcc agagctgcgg
     2641 cgcagcgcgt gccgtcgaac gcgcccagca ccgcgtgcac gatgccgccc tgtctgtgcc
     2701 cgggggcctt cagcaggacg gggacatact cccgggtgta cccggtgaac agcgtgttgt
     2761 tcaccggctt ttccagcagc acctcgacag gacgtccctc cattccggcg atgactcggg
     2821 cgcgcacagc gtccgcctcg gcctgcagcc ggtggctgcg gcgcgctttt tctgcttcgt
     2881 ctatctggtc cggcatatcg taagcgggcg tgccggcccg gcgggaaaaa ctgaacacat
     2941 gcaccttcaa aaagccaatt tcacggacaa aggccgcgct ctcctcaaaa tccgcctcgg
     3001 tctcgccggg aaaccccacg atgatgtccg tggtgaaaga cgcgcccggg aacgcggccc
     3061 gcagcttttc caccactgcg cgatagcccg cggtgtcgta aatgcggcgc atgcgccgca
     3121 gcgtggcgtc gcagccgctt tgcagcgaaa ggtggaattg ggggcacaaa gccggaacgc
     3181 gtgccatgcg ggcgatcatt tcttctgtca taaggtcagg ctccaggctc cccaggcgaa
     3241 tgcggcggat gcccggcacc tgcgccgcct tttccacgat ctctgccaga tctgttccgg
     3301 tgtcccggcc gtagctggag aggttgatgc cggaaaacac cacctcggcg tatccgccct
     3361 ttgccagtgc ggccagctcg ccgaggatgc tttcctcggc gcggctgcgc acgctgcccc
     3421 gggcgcgcgg aatgacacag taggcacagc gccggttgca gccgtcctct attttcatga
     3481 aggcacgggt atgcccctcc agacgttcca tgggaagctc ctcgaagcgt tcggattttt
     3541 catggggcgc aatgtccaca atacggccac cctcctgcaa aaagcgcagc acgttgtcca
     3601 gcacgcggcg gcgcgcgccg gagcctgtga tgatgtccgc ttcctctaca gccagcgctt
     3661 ccccgggaaa ggcctgagga aagcagcccg tcagcacggt caccgcgccg gggttccgcc
     3721 gcttggcgcg gcgcagccac tggcggcttt ttttgtcgcc gcccgccgtc acggtgcagc
     3781 tgttcacgat atacacatcc gcttccccgc cgtccggcgc gggctcgaag ccgttttcat
     3841 aaaaaagctg ggccagcgcg cccgtttcgt tttggttcac cttgcagccc agcgtataaa
     3901 atgcaacctt caaaatttca cctcagatca ggccgcgccc gaaacgcagg gcggtttccc
     3961 ggcgttttgg agcggcggtc atgaagctat tatacaaaaa ccgcgccgaa atcacaagag
     4021 cagtgcggac atccacgcat gcggggcgtg catgtttcat atagatgatt gaaagaccgc
     4081 gaaactgagg aggatggtta tgatggaacg gaaaaatggg atgcggctgc cggcagtcgt
     4141 tttgctggcc gcgcttgcct tttcgtgctt tgcccctgcg gcgctggcca cagcggagga
     4201 gccgcccgag gatctgcccg cagcagtatc cgcgcaggac ggcgcacagg aggaaacaac
     4261 gctgcccggc gcagacgccg cacaacagga cagcgccgcg gggaccggcg cggccaaccc
     4321 gcacgaaacc attgccacgt cggtgcagga aaacccgcgc ttcgcaaaac cgcagttgga
     4381 tatcccgtgc cgcaacgcga tcctcatttc catcgacact ggcgacatcc tgtatgaaaa
     4441 agagccggat gccgaggtgc ccatggcgtc tatcaccaag atcatgacgc tgctgctcac
     4501 actggaggca gtggaggcgg ggaagatttc catgacggat atcgtaccca tcagtgagca
     4561 tgcttttaat atgggcggca gccagatctg gctggagccg ggcgagcagt tcactctgga
     4621 tgagcttatc aaggccatct gcgtgtgcag cgccaacgac gcggccgttg ccgtggcgga
     4681 atttgtgggc ggctccgagc cggtgttcgc cgaaatgatg aacgcccgcg cggcggagct
     4741 gggcatgacg catacccgtt ttgtaaacgc ctgcggcctc gacgccgagg ggcattattc
     4801 cagcgcgcgg gacgtggccg ccatgtctct ggcgctgctg cgccatccca aaatactgga
     4861 atattccgga atctggatgg atacgctgcg gggcggcgag acgcagctca ccaacaccaa
     4921 taaaatgctc aagagctatg cggggatcac aggcctcaaa acgggcacca ccaacggcgc
     4981 gggagtgtgc atctcggcca gtgcggtacg ggacggtatg ggcctgctgg ccgtggtgct
     5041 gggctctccc tccagcaagg agcgttttgc atccgccacg gcaatgctgg attatggctt
     5101 cgccacgttt gaagccggcg catttcccca gatcgccggg cgtgcggaca cgctgcgtgt
     5161 gacgggcggc gtggaacagc aggtggcgct ggattacacc gtgccggaaa agatgctgct
     5221 gaaaaaaggc gagggccaga cgctgacggc gtccgttgtg cttccggaaa agctggacgc
     5281 acctgtggcg gcaggcacgc aggtggcttc cgtaacgctg aaggcggggg aagaaatact
     5341 gggtgaatat cccgtcacca cccgcagcga agtaccgcag atggactggg gcacggcctt
     5401 gtcacttctt acaaaagcgc tgtttacatt gtagaaaata cacaatatcg gcttgacacc
     5461 cgccgcccta tcctatatat ttagtatata tcaaacgaat ggttggaggc gctttcaatg
     5521 agtgtcaaaa tgaatggcag acaccttgca aaattcgtcg cggacgatga attcgacgcg
     5581 attttcccgc aggtcagcct ggcacacgaa acgttgcacg gcaagaccgg ccccggcagc
     5641 gattttctgg gctgggtgga tctgccggtg gattatgata aagaagaatt cgcccgcatc
     5701 aaaaaggctg cggcaaagat ccagtccgac agcgacgtgc tcgtcgtcat cggaatcggc
     5761 ggcagctatc tgggcgcccg cgccgccatt gaagcgctga agggaacgct ttacaacaac
     5821 acctgcaagg gaacgccgca gattttttat gtgggcaatt ccatcagccc cgcctatctg
     5881 cagagcgtgc tggatgcctg cgaaggcaag gatatttccg tgaacgtcat ttccaaatcc
     5941 ggcaccacga ctgagccggc actggcgttc cgcatcttcn nnnnnnnnnc gcggcacgct
     6001 gaaagaactt tccgcagacg agggctacga aacattcgtt gtgccggacg atgtgggagg
     6061 ccgctattcg gtgctgacgg ctgtaggcct gctgcccacc gccgccgcgg gctgcgacat
     6121 cgacgcgctg atggcgggcg ccgccaaagc acgggaggaa ctggccgtgt gcagcacgca
     6181 gaatgagtgc taccgctatg ctgcggcgcg caatatcctg taccgcaagg gaaagagcgt
     6241 ggaactgatg gcttgctatg agccgaactt cacgatgatg aacgagtggt acaaacagct
     6301 tttcggcgag agcgagggca aggaccagaa gggcctgttc cccgccagcg ttattttttc
     6361 caccgatctg cattccatgg gtcagttcgt gcaggacggc gcgcgcatca tgttcgagac
     6421 ggtggtggac atcaaacagc ctcagcgcga tttgtacatt gaaaaaagcg cagacaactt
     6481 cgacggcctg gatttcctgg ccgggcagga tatgagcgtg gtgaaccgca aggcgatgga
     6541 gggcaccatt ctggcccaca ccgacggcgg cgtgcccaac atcattctgg aggtcgacag
     6601 cctgacggag gccgaccttg gctatctgat ttatttcttt gagaaagcct gcgccatcag
     6661 cggttatatg cttggggtaa acccctttaa ccagcccggc gtggaaagct acaagaaaaa
     6721 catgttcgcc ctgctcggca aacccggcta cgaggcccag aaagcagagc ttgaggcgaa
     6781 acttcgttaa tcacaccgga cggtgttaaa ataaaggagg ctattcctat gattcaattg
     6841 attgttggtg gcaaaggctc cggcaaaacg aaaaagatga tcgacatgat caacgattcc
     6901 gcgaaaacca cccccggcaa tattgtatgc attgaaaaat ccatgaagct cacgtatgat
     6961 attgaccacg cggcgcgcct gatcgacgtt gacgagtata aaatttccgg ttacgacatg
     7021 ctgtacggct ttatcgccgg catcctggca ggcaactacg acattgtgga ggtctacctg
     7081 gacggcgtgc tgaagctggg caaccacgat ctggatggcc tcggcaagct gctggacgaa
     7141 ctgaacgtgc ttgcgggcga cggcgtaaag gtcgttgtca ccgtgtcggc ggacatggac
     7201 gtcctgcctg agagcgtcaa aaaatacgca taagaacaaa cgccatcaag ggcagggggc
     7261 gcggcaggcc gcgccccctg tttctgtgag attcgacgca agtttttgtg caaatggtct
     7321 tgacaagccc ccatattatg ggtataataa ctaggcaacc tttgaggagg ctcatcatgc
     7381 tgcttgacat caaaaagatt tttggtacgt atgacgagcc tgtcaccgca gcgctggaac
     7441 tggatctctc cgaagaagat ttcccgggat atacagtgcc gggcacggtg gaggcgcgca
     7501 tcactgcaac gcttcagggg cggatgcttg agatcgcgct ggatatcacc gcggatgtgg
     7561 cgtttgcctg tgcgcggtgt ctggaagaac gtacgcggcg gttccatgtc gtaaagacat
     7621 attgcgtgcg cgaggcggat ctgagcgacc cggatgcgga gcttagcttt acgcccgacg
     7681 gcaagctgga cgtaagagag cttgcgtaca ccgagcttgt gctcgaagtg cccaccgtgc
     7741 tgctttgcag cgacgattgt gcagggctgt gcccggtttg cggcaaccga aaaccatgct
     7801 cttgcaggca tgaaaccagc ggttccgtgg acgaaaggct ttctatattg aaacaactgc
     7861 tcaacgatta gattcacatc atgaggaggt gcttatagtg gcagtaccca agagaaaggt
     7921 ttccaaggca agacgtgata aacggcgctc ctctgtgtgg aagcttaccg cccccacgct
     7981 gacaaagtgc acgcagtgcg gcgaatacaa acttccccac caggtatgcg gcaattgcgg
     8041 ctactacaag ggcaaggaaa tcgtcaaaaa ggaagcgtaa tcagcttccc gcaaaaggag
     8101 agggagcgat ccttctcctt tttatttttg ccgcagaagg aggcgggcac atggcggtaa
     8161 agatcacggg cgcggacagg ggtacccctg cagagcaggc gggccttgcc ggcgccacgc
     8221 tgctgtccat cgacggcaac gagatcaacg atatgctgga ctatgagttc ttttcctccg
     8281 gcccgaagct ggagctggcc ctggtgcggg gcgcgaagct ggaatatgtt tgtgtggaaa
     8341 aggatgagta tgcgccgctg gggtgcgctt tcgagagcta cctcatcgac aaaaagcact
     8401 cctgccgcaa caaatgcatg ttctgcttca tcgaccagct ccccagcggc ctgcgggaga
     8461 agctgtattt caaggacgac gacgagcgtc tgtcttttct gttcggcaat tatatcacgc
     8521 tgacgaacct gaacgcccac gaagtggagc gtatcaaaaa aatgcacatc tcgcccatca
     8581 acatttccgt gcataccgtg gaaccggagc tgcgtgtgaa aatgatgacg aacaagcacg
     8641 cgggcgaggt gctgaaatac atcgacgagt ttgccggagc gggcattgag atgaactgcc
     8701 agcttgtgat gtgccgcggc gtcaacgatg gcgggcacct gcgcaaaagc ctggaaaagc
     8761 tgggcggcct gtacccggcg gtgcgcagca tcgcggccgt gcccacgggc ctgacacgct
     8821 accgcgacgg cctgtatccg ttggagccgt acgacaggga gagcgcctgt gtggtgctgg
     8881 atatccttga agaatatggt gaaaaatata agcggcagta cggcgtgcgc gtggtgtatc
     8941 ccggcgatga atggtttctg ttggcgggca ggcccatccc gccggatgaa tattacgatg
     9001 attatgtaca gctggagaat ggcgtaggca tgtggcgctt gctgcacgat gagttcatga
     9061 acgcgctgcc ggaccaaaag catacgctgc tgccgcgcgc catcgatgtg gccacgggta
     9121 cgctggccta tccgctcatc cgcatgctgg cggatacgct ccagagcctg caccgcaacg
     9181 taaaggtgac ggtccactgc atccggaacg attttttcgg cgggaacgtc agcgttgcgg
     9241 gcctcatcac cggcacagac ctgatcgcac agctgaaagg gaagctgcgc agccatacgc
     9301 tgggcatccc cgaggtgatg ctgcgggagg agaaggacaa attcctggac gacgttaccg
     9361 tcaagcagct ggaaaaggcg ttgggcgtgc gcgtgcgcat cctgccgcag aacggcgggg
     9421 aactgctgaa agccctgctg aagtgagaaa actgcggaag cctgatacaa aagaaaggaa
     9481 gacctatggg aaaacccatc gtagccatcg tggggcggcc gaatgtcggc aaatccacat
     9541 nnnnnnnnnn aggctggcaa ttgtcgaaga cactcccggc gtcacgcggg atcgcctgta
     9601 cggcaactgc gaatggcagg accaccgttt tctgctcatc gataccggcg gcatcgagcc
     9661 aaaagcggac gacgtgctgc tgcgtcatat gcgcgggcag gcgcagctcg ccatcgatac
     9721 tgcggacgcc atcctgtttg tgacggacat ccgcagcggc gtcaccgcgc aggatcagga
     9781 catcgcggcc atgc
//
LOCUS       DAWBSB010000071         9727 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1569_length_9727_cov_3.802523, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000071 DAWBSB010000000
VERSION     DAWBSB010000071.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 9727)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9727)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9727
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1569_length_9727_cov_3.802523"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1770..1779
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1671 a         2971 c         3246 g         1829 t
ORIGIN      
        1 cctgcgtggg ctgcggcgtg ctggcaagcg ccctgtgtat ggataaggac gcagccacgc
       61 cgagcagcag gcgctgtatg cggattccgt gcgggacaac ggccttgaat tcacacagaa
      121 atatgtggcg ctgcccggct gcagcgagca tgaaacaggc cttgccattg atgtgggcga
      181 ggcgcgggag gtgatcgact tcatccgtcc cgcatttccc gatacaggag tctgcgcggc
      241 cttccgccgc gcggcggcgc gctacggctt catcgaacgg tatcccaaag gcgcgcaggc
      301 cgtcaccggc atcggccatg aaccatggca ttttcgctat gttggctggc cccacgccgg
      361 gctgatggca cagcgcggcg tgacgctgga agaatacatc ggagccttgg gggcatacac
      421 gcccgaacag ccgctgcacg cggaggccgg gggacgcggc tttgacatct ttcgtgtgcc
      481 gctggggccg gagggcgcgc ggttcgacgc gccgcgggac cgtgtgtggc aggcgtccgc
      541 agacaactgc ggcggccttg tcgtcaccgt gtggggcacg gtatgacggc gcggcgggga
      601 tatccggccg tggaccgctt ccgcgtggcg gccgcgctgc tggtggtgtg catccatacc
      661 gctccgctgg cgtctttttc cgcagcggcg gattttgcgc tgaaaacgct ggcgcggctg
      721 gccgtgccgt tctttttcac ggcaaccgga ttttttctgc tgggggagct tgtgcgcccc
      781 ggcctgcggc cccggacgct ttcgcccggt gtgcgccgct ttttgaaaaa gacgggcgtt
      841 ctgtatgcag cctgcaccgt gctgtacctg cccgtgacgc tgtatgcggg caatttccct
      901 gtgaaaaacg tgctggggga gcttgtgcgg gacgctgtgc tggacggcac gttttatcac
      961 ctgtggtacc tgcccgccgc gctgctggga gtgctggtgg tgtgcgggct gctgcgcttc
     1021 ctgcgccccg gcgcggcggc gggcgtgtgc ggcgcgctgt atctggcggg gcttttggga
     1081 gacagctggt atggccttgc aaaacagctg cccgcactga aagcgggcta tgacgcgctg
     1141 ttcggcgtga tggactacac gcgcaacggc ctgttctttg cgccggcgtt tttgatgctg
     1201 ggcgcgcttc tggcaggttt tcccgcccgc cgacgaccgc gggcgtgcct tgcggggctg
     1261 ggctgttcgc tggcgctcct gtttgccgag gcctttggtc tgcgggcgct gggctggccc
     1321 cggcacgaca gcatgtacct ggcgcttgtg ccctgcgcct gcttcctgtt cctgtgcctg
     1381 ctggtccccc ggggcgggga cgcgccgttc ctgcgggaat tttccatgct cgtctatgtg
     1441 ctgcatccca tggccatcgt gggcgtgcgg ggtgcggcca aggtgctgca tgcccgggaa
     1501 tggctggtgg aaaacagcct ggcccatttt gcggcggtag ccgccgcgtc ctgcgcggcg
     1561 gcatggctgc tggcgcgttt gagccagcgg cgcaggtggg acggccgttc cggaacagca
     1621 ccgaagccgg acttgcgcag agcgcgtgcc tgggccgagg tagacctgga ggcggttgcg
     1681 cgcaatgcgg gcgcgctgca gggctgcatg ccagcgggct gccggctgat ggccgtggta
     1741 aaagcggacg cttacggcca cggcgcgccn nnnnnnnnng cgggccggct gtggcaggcc
     1801 ggtgtgcggg cctttgcagt ggcgacgccg gaggagggcg cgcagctgcg ccggtgcggc
     1861 atcaccggtg aaatattggt attgggctat gcagatgcgg cccgtatacg ggagctgcgg
     1921 cgctggaggc tgtgccagac ggtgacggac ccggcccacg cccgtgcgct ggcccgtgcc
     1981 gcaggccgca ggccgctgcc cgtgcacatt gcggtggaca cgggcatgca tcgcctgggc
     2041 acggatgcgg gcgcggctcc ggcggtggcc gagatgctgc gtctgccggg gctggaggtg
     2101 cgggggctgt tcacacatct ggcagtggcg gacagccctg cgccggagga cgcggccttt
     2161 acccgcacgc agctggatgc gtttgaagcg ctggcgcgga agctgcgggg cgcggggtat
     2221 acgctgccgc cgctgcacgc gcaggcgagc tgcggcatcc tgaaccagcc gcagcctgac
     2281 tgcggatatg ccaggccggg catcgcgctt tacggcgcgc tcagtacggc gggctgcgcg
     2341 gcgcggctgc acccggtgct ggctccggcg ctggcgctgc gggcgcgagt cgtcagtgtg
     2401 cgccgcgtgc cgcccggcga aggcgcgggc tatggccttg cgttccgggc acggcgctcc
     2461 acccggctgg cggtagtggc catcggctac gcggacggct ggccgcgcgc ggcgggagaa
     2521 gggcgcggcc atgtgctgct gcacggcagg cgcgcgcccg ttgtgggccg ggtgtgtatg
     2581 gaccagcttc tggtggatgc aacggacaca ggcgcggcgg tgggggatgt ggcaacgctc
     2641 atcggcgcgg acggcccgga gcgcatcacc gccgaggaag cggccgaggc ggcgggcacc
     2701 atcgccaacg agcttttgtg ccgcatcggt ccccgcgtgc cccgcgttta tctgcgctga
     2761 gaaaacgaaa gccccgcggc gttgtgcaca caacgccgcg gggctgtttc ttgcttcaca
     2821 ataaaagctt atccggacgg tccgcatggc ctgaaaaata gcctgtacgg ccattttgga
     2881 agcgggctca ccagcgcccg ccgccaccgc cgccgaagcc gccgccggaa aaaccgccgc
     2941 cgctcccact gaagctgccg ccgccaccgc tggagccgcc gctctgctgg gcgacggttt
     3001 gctgcagctg cgtcatggaa tggtctacgc tgtggtgcat catatggctg aaccagatca
     3061 cattccacac ggtcatgtcg ctgcagccgt accacacggg cggaggcacg gccatcccat
     3121 caaagcgccc ggcccattcc tcctccagcc caaacaccga ggcgtaggga aggacgtcgt
     3181 aaaaatattc ggggttttct gcggccagcg cacggatgcg cggcagctcg gattcctcaa
     3241 tgaatttccg caggcccagc aggcggccgg ccatttccag gcggtactct gtgggctggg
     3301 cgatgcgcgg cgcggcaagg cacgccagca gggccagcag atagcttccc agcaccgccc
     3361 acagcggcgt cagcgcatac agggccgcgc cgtaggcaag ccccgccgac gcggcggcgc
     3421 acaggcccac gccggttccc cagccgatgc gcgccccccg gccggagaac tgccagcgct
     3481 ccgccgccgc atacagcagc aggccgaaca gcaggagcaa tacccccgag agcaggccca
     3541 gccctacgct ggcacctgtg ataaacgcgc cgcaggaaag gccggccgcg ccccacagta
     3601 cggcgcagcc agcgcccagc gccagcgcac gtctggcgct gccgggcctg tacaatgccc
     3661 gttcacccgt aaagtgggcc gacaggctca gcttcgcttt ctgcagcgcc tcatagaagg
     3721 cgggctccag gctgtgcagc tggcacacgg tgcgtcccgc cgggaaaagc gcgtcaaaaa
     3781 agacggtctg gtactccggg ctgcccgcgg gcaggccttt tttccgtacc agccgcaggt
     3841 cctcctcccg gccctctacg gcgagatagc ccttggaggc gaaccagaga atcagcgaga
     3901 ggatgtcccg gtcatccgcc gaattgtcga tgatatagcc cacctcggcg ctggacacgc
     3961 cgtctggcgg cgtacactcg ggcgtgacca ccggcgctct gcgccgggcc gacagcgccc
     4021 gcagcagcgt gtacgcggcc agcagcgcgc cagcgcccgc aaaagcccaa aacacccata
     4081 ccggggccat gcgctcgccg gtgaagtagc cctcgggcaa gcgtgcgtac agcgtaacgg
     4141 cctcgcccgg gtacagcgtg cgccccgccg cgccggagaa tgtgtttccg tcccatgcgc
     4201 cggaaacgcc cgcttcattg cctttgccgc cgtaggagcc gctgtacagc gccagcgcgc
     4261 ccttctgttc ctgcgtcagc ggtttttcaa aggtgaacga aaaacggaag tcctctatgg
     4321 gcgcgtccca ccggccgccg tttaaggaat aaaacagctc gtcgtaggcg gctacgcggt
     4381 cgtctccgat gtcgtaggtg aatgtcagct cataggtctg cacgcctgtg agccatgtgt
     4441 cctcatcgcc gatgcggatg ctgtaaaagc cgtcttcggt atcggtctcc acgggcgcgc
     4501 cggccgtctg gccctcgccc gtcacggcaa ggtcccgcac cctggcgcgg taggccatct
     4561 cctgcgggcc gtccgcggtg tccttttcca cccacagggc gacgggcagc acgcggtaaa
     4621 tacctcggga gggtacggcg aaatcaacgg tgatgcgctc ggtctgggta acggtgttgt
     4681 tttcgtgcag ggtggcgtcc acgctgtatg cggtaatggt ataatccgcg cgggaaacgc
     4741 cggaagcttc ggcaaatgcg ggcagagcgc agcatacaag cagcgccgcc gcggccagca
     4801 ggcagatgag tttcttcatg tgctttcctc cgcttttttg gtttgcccgg tacggcaaag
     4861 ccaatactgc caacagtata acacaatatg cgcggcgaaa acagcgggaa cttgcaaaat
     4921 aacgccggat acggtatgat aatgcctgtg cacggccctg ccgcgccgag agaacgccct
     4981 gaaacagcag ggcggaaaat ttgaaaaaag caaaaggagc aaacggatgc ttgcactggt
     5041 aaaagaatat ttttggatct ttttcatttc catggtgccc atcgttgagc tgcgcggtgc
     5101 ggtcccggcg gggcttgcca tgggcctgcc ggtgattccc acctatattg tttcggtcat
     5161 cggcaatatg ctgcctgtcc ccgtcatcct gctgtttgcc aaggcggttc tgctgtggtg
     5221 taccaagctg cccggcgggc tgggccgttt ttttatgaaa atttacgaca agggcgtcaa
     5281 agccggcgaa aagatgcagg cgaaggccgg gcgcggaatt tactgggcgc tgttcctgtt
     5341 tgtggccatc ccgctgccgg gcaccggcgc atggacgggc tgcctggcgg cgacgctgtt
     5401 ggacctcaag ttttggccca gcgtgctggc ggtgctgtgc ggcgtcatga cggccggcct
     5461 catcatgggc gcggcctcgg ctggcctgct gggcgctctt ggcatgattt ttgcgtgaac
     5521 ctgttacgca gcaccctgcg gggggatttc cccccgcagg tttttttgat tttcggcctt
     5581 tccggacaag cggcaacagg gcaatttctg gaaaagtatc aaaaacttta tttttcttga
     5641 agaaaatgat cttccggcgg cgtttcatgg gtgtagggcg atgaaacaag ggcgggcaaa
     5701 ggtggtggag cggatcgcac agaatatgac aagtgatgaa ttttcccagc tcgtacggca
     5761 gtacgaacgg ctggtgtaca ccatctgcta ccagttcaca aaagaccacc agctggccga
     5821 ggatcttgcc caggagacgt tcctttccgc ctacacccac cgcgacgact gcccgcccgg
     5881 cagttacaag ccctggctcg cccgcattgc cacgaacaag gccaaggatt atttgaaaag
     5941 cgcctaccac cggcgcgtat ccgcggctga ggacggcgag atgcctgccg tggcagacgt
     6001 gctgaatacc atgccgccgc ccgaggatat caccattgcc aaagatgaag cggcgcgcat
     6061 ccgggatacc atcctcgcct tgaaagagcc ttaccaccag gtcgccgtac tgttcttcct
     6121 ggaggaaaaa agcgtggacg agatcgcctc ggcgctgcgg cggccgccca aaacagtaca
     6181 tacgcagatc taccgcgcca agcaattgct gcagaacgcc atagaaaggg gaaacgggca
     6241 tggaattgtt caataaagag ggccatctca cggacgaggg cctgcgggcc gttgtggacg
     6301 gcacgctgga tgagatgggc cggctggaag cctcggagca tttgagcttc tgcgacagct
     6361 gccttgtgcg ttacacggaa ctgcttgcgg acgacacgct gctcgttccc gaaatgccct
     6421 tgacgcccac ggtgctggcg cgggtgcggc gcagggccgt gcgggtgttt ttcaaccggt
     6481 atacccgtgt agccgccgtt gcggcattcg cggtggtgct gtggggtact ggcgtattca
     6541 acagccttgt gccggaccgc accatccgcc agcaggagcc ggcccggatc acggcgccca
     6601 tcagcgccgc aacgcgcgtg aatgatttct tccgcagtgc gggagactcc atcagcgccg
     6661 cgctcacgaa tatcctgccg cgcgcggacg ccgccccggc agatgcgaaa taagacaagg
     6721 gagagacata tcatgcaaag aaaaagtgga tttctgacat tctgcttcgc ctgcctgccc
     6781 ggcgcgggag agatgtatct tggttatatg aaacgcggcc tgtccgtgat gattgccttc
     6841 tggggcctca tctttgtggc gtcgctgctg aatatgggca tcctgggcat ccttgcgccc
     6901 atcatttggg cttactcgtt cttcgatact ttcaatctgc gcgcgcagac gcccgagcag
     6961 gtggcggcaa acccggacgc ctatctgttc gatgtggaga gcattgccgg ttccaactgg
     7021 aaaaatgttg tggccaggcg tcacaacctg ttcggcggcc tgcttatctt tctgggggcg
     7081 tacattctgt acaacacatt tctgcgtcct ctgctgtggg atctgtaccg cacctatggg
     7141 ctggtctggc tgggcaatat catggacggc attccaaccc ttgtgatcgc cgtgctcatt
     7201 atcctgctgg ggctgtatct ggtaaagggt ccttcccggc acgaggatgc atccggggat
     7261 gattacaccg cgttcaaggg ggacggaagc aatggctgag aacgagaacc gggtctccgc
     7321 cgtgcaggag gctccggatt caccgcagga gccggcggct ttggataccc cgccctctga
     7381 aatgccggtg tacgagccgg cgcgcccggt gcgccgggtg ggcacgctca ccatgggcct
     7441 tgcgctggta gtggtaggcg cggcgctgtg cgtggggctg ttcttcccca atgtggattt
     7501 tcttctgctg ttcaagctgt cgccgttggt actggtggcg ctgggctgcg aggtgatctt
     7561 cgcggcgtcc acagccaaag gcatgcggct gaaatatgac tttctctcca tgttcgtgtg
     7621 ttttctgctg attgtcacag cgctgggagc ggcatgcgtg cctgtggcac tgcaatatgc
     7681 cgggccgggg cgcagtgctg ccgagcagcg ggtggagcag gagctgtacg aagcgaccta
     7741 cgcccggctc aagggcaacg gagatattgt gaatgtcacc tatgacgttc acctgggtga
     7801 gctgcgcacg ccggaagacg tgaccggcgc ggcggatctg aaggcgggcg attacgtggg
     7861 tgtgagcgcc gagctgaaag gaacgtgggc cacccgggag gaatttgtgg ccgcgtgtcg
     7921 gcctgtcatt gaagccgtgc gcgccacggg cgtgcgcgac ccgtatatca gcctgtatat
     7981 gcgggaaagc cgggatacag aaacaccgct gtacagcctg gccgtggagg gatcctatca
     8041 ggcggatatg acggcggagc agctggccca gtgcgtgacg gaacgcatct atgtggatga
     8101 cgcgggatat tatatggacg ccgaagagct tgccggctgg cagagcgaac agagcagcgt
     8161 tgcccgtgac gccgagcttg aggcgctgga ggccgagtgg caggcccgct tggatgaagc
     8221 cgaagctgcc gcggagcaag cccgtgcgga agcggaggaa cgcgttgcac aggtacaggc
     8281 cgaagccgac gagcgcgttg cgcaggcaca ggccgacgcg gacagccgcg tggccgaggc
     8341 gcaggcaaac gccgcagcgt aagcgcgtct attatataga aaacagcccg ccgcactctt
     8401 ttggaacgcg gcgggttttg ctttaccttt ttgaacttgt tcaagccccg gctttgccaa
     8461 gcggtatggc tgcggcctcg gtacgggcac ggcgtggcgg aaagcgtccg cgactgccgc
     8521 gccgcacgcg gtcacacccc ggccagacgg aggatggctt cctgccgggt ggcggggaaa
     8581 aagatatccc ggccccggtt gctctcgcgg ataaaatccc gcaggggttt gctggtatat
     8641 ccggaataat cgccgtacac cgccagcttt gcgccgtaat tgatgaattt ttgcagtacg
     8701 gccccggcaa ggcccgtgct caaaatgaaa aaatccggcg aaacagcggc cttttcaatg
     8761 gcgatgcgct ctgcgcccag tgtgtagcgc gcttccatga tcaagtccag cgccgtctgc
     8821 tcgtccgtca gtgcgcacgt gcctgttaca agggcgatgg cgactccgtt ttctgtgatt
     8881 ttttccattg ccatgctctt tcctcctgtt ctgtttgaat atgccttctg ctcaaaggct
     8941 tgccgtcagc cattgccgca gagcctccag ctcctttggc gtgtggaaat agtggcccgc
     9001 ttcggggcgt gtcagcagcc tgcagccgta ttgccgggca aaacgggcgg tgacatccgg
     9061 ctcacacagc tcgtcccgtc cgccgcacag aatggatgtg ggcgtgtccc agcggcgcac
     9121 gggatgttct ttcacatagc agtagtaatc ccagtacagc gtttctccgg tgggtgtttc
     9181 tatggcccgt tcccggcaca ggcgctcctg tgttacgccg aaccagccca tcatgttttc
     9241 aatgagccgc cgcatatcca agacagggga aaggaacagc gcctgttcca gcggctcgtc
     9301 cgcgtaggct gccaggctga agcaggctcc caggctgcag gcgaacaggc ccacgtgcgc
     9361 ccagcgcttt ttggcgtacc ccatcacggc cctaagctcg ggtacgcaga cttgtatcct
     9421 gcacggcgca ggctcgtctt tgcggccgcc gtgtccgggc agatcgaagc tgagcacctg
     9481 cagcgcccgg gcggacgcgg cttcggcaag cagctgaatg ggcgtatcgg ctttgtggga
     9541 caaattgccg tggacggcaa cgaccgcccc atcgctcggc ggtccccaca caagggcggg
     9601 gattccgccc agatcgagtt gttctgtctg cataaagctc cttcctcgga taaaacgggc
     9661 cgtggacgtt ttccggccca tgatcagaga cggaaaacaa gaaaacccgt gccgcattgc
     9721 caggaag
//
LOCUS       DAWBSB010000072         9715 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1571_length_9715_cov_2.725466, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000072 DAWBSB010000000
VERSION     DAWBSB010000072.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 9715)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9715)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9715
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1571_length_9715_cov_2.725466"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    7938..7947
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8273..8282
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2015 a         2715 c         3095 g         1870 t
ORIGIN      
        1 tgccagagct tcatgacgga gctttccaag aatatcggcc agtttaccga cgtgcccgcc
       61 ggagacatcg gcgtgggcgc gcgggagatc ggttatatgt tcggccagta caaacgcctg
      121 cgcaacgaag tgacgggcgt gctgacgggc aagggccttt cctacggcgg cagcctggtg
      181 cgtccggagg ccaccggcta cggcctgtgt tactttaccg aggaagccct caactgcatg
      241 aaaaacgaca gctttaaggg caaaacggtg gtgatctccg gcagcggcaa cgtggccatc
      301 tacgccacgg agaaagccac ccagctgggc ggcaaggtcg tggctttgtc cgattccacg
      361 ggctatgtct acgacaaaaa cggcatcgac ctggatgtgg tgaaggagat caagctggcc
      421 cgccgtgccc gcatctccga gtatgttaag gcggtgcccg ccgccgagta tcacgaaggc
      481 tgcagcggca tctggagcat cccctgcgat atcgcgctgc cctgcgccac gcagaatgag
      541 atcgacgagg ccagcgccaa gctgcttgcc gcaaatggct gcaaggtagt gtgcgagggc
      601 gcgaacatgc cctccacgcc ggaggcgatc aacgtgtatc tggaaaacgg catgctctac
      661 ggcccggcca aggccgccaa cgcgggcggc gtggccacca gcggcctgga gatgagccag
      721 aactctctgc gcctgtcctg gacattcgag gaagtggacg agcgtttgca gaatatcatg
      781 aagaatattt tccacaatgc ttatgacgcc tccaaggcat gcggtgcgga aggaaacctg
      841 atggtgggcg ccaacgtggc gggctttatc aaggtggccg acgccatgct cgccgagggt
      901 attgcgtatt aagcatcaac cgtacaaaac cgggcagggg ccgcacagag cggcccctgt
      961 tttttgcctg ccgggacggc ggggcatcca tgggaaagga gcggagcgca tgacgctgga
     1021 atttacggtg gagcaaaagg aaaacggtat gctgctgcgg gactttctgc gcaaacgcgg
     1081 tgtttcggca gcgctggcca agggcgtgaa ggcctcggac ggctttttcc gggacggcgc
     1141 gcccgtacac acggatatgc gcctttcagc gggccagcgg atttcctttg cgctgcctcc
     1201 ggagcctcct acggaggtgc tgccccaggc gctgccgctg gatatcctgt atgaggatgc
     1261 ccatgcaatg gtgctgaaca agcccgcggg acaaacggta catcccaccc gggggtatgc
     1321 ggacggcacg ctggccaatg ctttccgcgg ccggatggcc gcgcggggca gcgcggccgt
     1381 gttccgtccg gtgaaccggc tggacagggg aacctccggc cttgtgctgt gcgcgatgaa
     1441 cgcctatgcc gcgccgctgc tggccgccgc ggtgcaaaaa gtgtactatg ccgtggccga
     1501 ggggctggtg gacggggagg agggcgccat cgacgcgccc attgcgctgg cgcacgggtc
     1561 catcattcag cggtgcgtgt gcggccgggg gcagcccagc cgcacggaat accgggtgct
     1621 ggcacggggc ggagggcaca cactgctgcg ggtggtgccc gtcacggggc gcacacatca
     1681 gatccgtgtg cacttcgcgt cccaggggca tccgctggcg ggcgacggca tgtacggcgg
     1741 atgtccggag cacatggcgc gcccggcgct gcactgcggc gcggtatcgt ttggcctgcc
     1801 gggggaggat gcggcgcgca cagtgcacgc gccgctgccc gaagacatgc gtgcgctgct
     1861 ggcggcgcgc ggcatccggg cggaagattt cggcatttga ccccttccgt gtgttgatac
     1921 aaatgtgaaa aaaggtatgc gtcgccgcgc tggaaaggga ttaaacttgc atcgtgggca
     1981 aaatagtgta taataataaa gttcggaccc ccgctctgac cggcgctcct gtgccgggga
     2041 tgcagatgga accggacatt tgcctgtttc tgcaccgcgc tttgcgcggc tggcatatcc
     2101 ccacggagac ccggaaagga gggggcgtat ggctgcaagg aaacgctggc gcaggtatat
     2161 cccggacgac gagatatccg tgcgcgcttt cctgcatttg atcggccgcc tgttttatta
     2221 tgtcggcctt cattcggaat attttctgct gaacttcggc cgcggcgcac gcgccgccgt
     2281 actggcgctc tggcacggcg tgtgctggat gctggcgctg ggcgccaata ttctgcggcc
     2341 gttttttgcc gctgtatggg aggatctgcg cgccccttgg cggcagatgc gcagcgggct
     2401 gcgcaacatc cgccaggtag tgcgtgaaga acgggaggcc ggcggctcgc cggcgggcaa
     2461 aggacttgcc tatttcttcc gcggcgtttt ggcgtacaag cacctgctcc tgcgcggtct
     2521 ggcgtatctg ctgcccgcgg ccgcgctggg cgtgctgctc gttaccgtgg acggtgttct
     2581 gtcctctact tttgcactgc gggtggaatt ccgcggcgaa ttcgtgggct tcattgccaa
     2641 cgaaagcgtt tatgatgcgg cgcacgcgga tattctggac cgcatccaga atgtggacac
     2701 gggcgaggac tggaaagcgc gcccggaata tacgctcacc attgtggatc aggcggcgct
     2761 gtattcccaa ggcgaactga cggaccgcat catcgagaca tcatctgctg aattccgcga
     2821 ggcgacaggc gtctatgtca atacggagtt catcggcgtt acggcggatt ccgccgcgtt
     2881 gacacaggcg ctggaggctc tgaaagagcc tctttacgaa ggacatgaga acgacgacag
     2941 ctggcgcgtg gagttccagc aaaaggtgga acttaagccg ggcctgtatt tcaccagcac
     3001 catcgtcacg ctggacgatc tgctggcgcg cctgcacgga acggctcccg tggagctttc
     3061 cgacggcaat atcctggtgg gctgggatat gctgggcgtg caggcggtgc agaatgtgac
     3121 ccgcaccgtt gaaactgcgg ccgagccgca ggtcatcaat gaccccgagc tggagtgggg
     3181 ccgcgaagtg gtggagcagg aggcttcacc gggcctggag gaagtaaacg aggacgtcgt
     3241 ttatatcgac ggccaggagg tacagcgcat tcagacgcgt gagccgaccg tgctggtgga
     3301 ggcggtgccg aagatcacgc ggtacggcac gtataacccc tacggcggca ctgcgggaga
     3361 cccggcgaca ggtgaattca cctggccggt accggattat aagggcatca gccgctgggc
     3421 ctcacgctat caccgcgggg cggatatcac ggcgcgttac ggtacgacca ttgttgcggc
     3481 ggataacggc gtggtggaag ttgcgacaga cgcgcgcggc acggcatggt ggacgtacgg
     3541 gaaatttgtc aagatcgacc atggcaacgg cttcgccact gtgtatgcgc actgctccga
     3601 gcttctggtg cagcagggtg aatatgtagt gaagggccag cctatcgcgc gcgtgggttc
     3661 cacagggtat tccacgggca accactgcca ttttgagatt caggtgaatg gccagtggac
     3721 agatacccga aggtatgtca tgccctaagg atacaccacg caattcatcg gcgtgcttcc
     3781 ccgcttaggc ggcgtaggcg cgcggagaat tgaccgagtg ccgttcgtga acacacaagg
     3841 ccgggcgtac agcgcccggc ctttacattg gtgacaaaaa attgacgaat catgccgttt
     3901 ttgttgcaaa aagcccggca aatggatata ataagtgcgg ttgtgacaga gcctttccgc
     3961 actttcagtt aatttgtgcc aaatcctgtc gaatatgcat ttatgctttt aatctgcgca
     4021 aaattagtgt atactaagag agttaccatg cgctctatgc gcggcctttc ggcccgatcc
     4081 tataaagaac gctcaaagga gagtacgtct attggaaaag tttgtcattc acggcggtaa
     4141 accgctggtg ggcgaagtcg agatcagcgg cgcgaaaaat gcggcggtcg ccatcctgcc
     4201 ggccaccatt cttgcaggcg gtaaatgtgt gatcgaaaac ctgcccaata tcagcgacgt
     4261 cagtgcctct gtggacatcc tcagcgccat gggcgcgcat gtgcgcgtca tcaacaagca
     4321 cacgctggag atcgacacag cccatatcac cgccacaagt gtgcccaacg agctttcgcg
     4381 ccagatgcgc gcctcttatt attttctggg cgcgctgctg ggccgtttcg gcaaagccac
     4441 cgtggccatg cccggcggct gtaatctcgg cccgcgcccc atcgaccagc acctcaaggc
     4501 gtttgtggcc ctcggcgcga aggactcggt ggaatacggt atgatcagtg tggagtccga
     4561 cagcctgcgc ggcgaggacg tgtttttcga taccgtttcg gtgggcgcca ccatcaacgc
     4621 catgctggcg gctgtgatgg ccgagggcca gaccgtgctg gaaaacgtgg ccaaagagcc
     4681 ccacattgtt gacgttgcca actttttgaa tatgatgggc gcggacgtgc gcggcgcggg
     4741 caccgacgtc atcaagatcc gaggcgtcaa gcagatgcac ggctgcacct attccatcat
     4801 cccggaccag atcgaggcgg gcagctacat ggttgccgcc acgatcacag gcggcaacgt
     4861 actcatcaaa aatgtcatcc ccaagcatct ggaacctatc accagcaagc tggaaaaggc
     4921 gggcgccacc attgaggagt tcgacgattc cctgcgggtg taccgcacgg gcgacctgga
     4981 gccgctgaat atcaaaacaa tgccgcatcc cggtttcccc accgatatgc agccgctgat
     5041 gaccgtgctg ctcacattgg caaaggggac gtccatcatc accgaaggta tttgggaaaa
     5101 ccgttttcgt tatgtggacg agctgacccg tatgggcgcg aacatccagg tggacgggcg
     5161 cgtggctgtg attgagggcg tgcaggagct gctgccggca cccatgcgcg cggcggacct
     5221 gcgcgcgggc gcggcactcg tggttgcggc gctggcggcg gacggcgttt ccgaagtgga
     5281 cgagatttgc cacatcgaac gcggctacga ggatatcgtg gaaaagctgc gggggctcgg
     5341 cgcggacatc aagcgggtgg aaaagcccgc gggcgtgctg cggcaggctg cgctgtaagc
     5401 ttacaaaaag cagaacggaa ggagcgtttc ggctccttcc gtttttgaat gttaaaaggg
     5461 aggctgcgca tggcgcgttt taccacgctg tattccggtt cctcgggcaa tagcgccctg
     5521 gtggaggaaa acggccgtta cttactggtg gatatgggca agagctgccg catgacgcag
     5581 aacgcgctga aagggttggg gctgccgctg gcggacctgg gcggcatcct tgtcacccac
     5641 gagcattcgg accatgtgag cggtcttgcc atctttttaa aatatcaccc tgtgccggtg
     5701 tacggcagcg cggccacgct ggattatctg gcttgccggg gccttgtgcc gccgggcgcg
     5761 gaattgatag acatcgacgg ccgcaccgag gacgtgtgcg gttttgcggt gcagagcttt
     5821 gaaacgagcc acgactctgt ggcctgccgg ggttaccgca tcaccacgcc tttgggccgg
     5881 gtggcaggcg tcgcaacaga ccttggctat gtgagcgacg aggtgctggc gaacctcttg
     5941 ctggcggatg tggtggcgct ggaagccaat tatgattata atatgctcat gacggggccg
     6001 tatccatatt atctgaaaac gcgcattgcg tcccagcgcg ggcatctgtg caatgaggag
     6061 aacgcggcca cgctggtgcg tctgctggaa aacggatgtg aaaggttcgc gctgtgccat
     6121 atcagccagg agaacaatac cccggagctt gcgctggaaa gcgtgcgcgc cgcgctgctg
     6181 ggcgcgggcg ttgtgccggg gcgggactgc gttgtgaatg cggcgcgccg ccacgaggta
     6241 tcgcccatca ttgaatttta agcgcgccgt gcggcagccg caggataaca ggagggaagc
     6301 attgtgcaga ccattacgct catcgcgctg gggaagctga acgcggatta ttacgcaaag
     6361 gccgccgccg aatacgcaaa gcgtctttcg gcctattgca ggctgaacat cgtggagctg
     6421 ccggaggagc ccattgcgga aaaaaacgct tcgcccgccg tcatcggcaa ggcgctggaa
     6481 aaagaggcgc gcgccattct ggctgccgtg cccaagggca gcggcctggc ggcgctgtgc
     6541 gtcgaaggaa aacaaaaaac cagcgaggag ctggccgcgt atctggacga gcgcgcgggc
     6601 agcggcgcgg gggacgtggc gttcgtgatc ggctcgtccc acgggcttgc ggacagcgta
     6661 aagcagacgg cgtctctgcg gctttccatg agcaaaatga cattcccgca ccagcttgcg
     6721 cgggtgatgc tgctggagca gctttaccgc gcgttttcca tcaaccacgg cggaaaatac
     6781 cacaagtgac ccaaaagcgc cccgcgcacg gccggacgga caccggaccg atgcgcgggg
     6841 cgcttttttg cggctgccgc tccgggcgtt aatccggacg gcgctcccgg tatgcgagcg
     6901 cttcgtcggg cgctttcatt ttgccgatgc agaacagcag gacggcaacg cccagcaggg
     6961 cgaggatgca ttttccaaag gttttcatag agggcttcat cctttcgttt tgaagtggag
     7021 caaagaggcg tcacgcctcc acaaggcggt aataggtatg cgcggtaatg cggtatacca
     7081 ccacatacgc ggcggcgaaa atgagaaatg tgacggccgt gcagccgaag aacaaaagcc
     7141 ggttcaccat tccgaacaca agaagcatgt tgcagatgat ctcaaaggcg aacaccatat
     7201 gcagcgcagc catgagcagc ggcaggaaaa acaccagcaa tatctgtttg ttgatggcgc
     7261 gtttgacctc tttccggctc atgcccacct gctgcatgat gcggaaccgg tcgtgatcct
     7321 catagccctc gctgatttgt ttgtagtaga tgatcagcac cgtggccatc aggaacaggc
     7381 accccaggaa gatgccaagg aacaggaagc tgccgtacat ggcgttccac tcggtatata
     7441 gctcggagcg ggcataaatg ccgcagatac cgttaagccg cgtccataga tcctcctgca
     7501 atgcggcgat ctgctccggc gtggcgtcca cgtcgaaggc ggcgctgtaa gcgaactgtt
     7561 cctgcgttcc gcccatggca cggtacaggg aagtggcttc ttcatccgga acaacgagga
     7621 taaggccggt tatggcggtg tagttgccgg agccgatata agggctctcg tccagccgct
     7681 gtgcgatatg aacagcctgc ccgccgagag taacagtatc cgaaagcaca aagtttcccg
     7741 tgggataggc aagggcttcg ccggaagcga gctggaagtt tgtgccgaaa gtagaattat
     7801 agtctgaaag agggacaagt gagaccgcaa caacctctcc ggaactcacc gaagcaggcg
     7861 cgtcttcgta ggaaccgtcg acagaaatca cattaagact gcggctgcgg ccggcgatgg
     7921 gatttttgat ttccaggnnn nnnnnnnaaa agcgtcctga accgccggcg cattttccgc
     7981 tgcggtagaa ttgctgcaaa tttctctggg gtacatgctt gtcaccatgt cattgcgccc
     8041 catgtacagg cttacggtag tagagatcgt gacgagcacc atcgtagaaa ggatgcagat
     8101 ggccgcaagg ccggcggcgt tctgcttcat gcggtacatc atgccggaga cggcaatgaa
     8161 gttgttgggc ctgtaataga aattcctgcg gcgccgcagg gctttcagca gagcaatgct
     8221 gccggcggtg aacaggcaga acgtacctag aatgacgagg aatacagcga cannnnnnnn
     8281 nncccgcaaa ggtgagaagc caggaggttt ttggctcttt ttcgcctttc tgcccgccgt
     8341 ggagcagctc caccgggttg gcaaggcgga tgtgcaccat gttgtacagc agtgtcgcgc
     8401 cgtagatgca cgcgaacagc accacagtgg tgacgagcga cggcaccgag acggagaacg
     8461 ccagcggcgt tgcgaaatcc agcagataca gcagcgccat gaacagcagc tttgagaata
     8521 atacgccgaa cacaagcccc agcgccagac acgcaattgc ggtgaacagc gtttcaaaaa
     8581 acaaaaccag cgcaacgtgc cgcttttcca tgcccagtac acagtaaagg ccgagttcct
     8641 ttttgcgccg cttgatgaga aagctgttgg tgtagaacat cagcgcaatg cagaaaacag
     8701 caataatgcc cgtgcccaga aaaaagatgg tcatggcgat ggcggcgccg ggcacctcca
     8761 tcagcccctc gttcatggcg atggactgca tgatatagta cgtcatgatg catacgatac
     8821 tggtgagcat atagggaaag taggtgcttt tgtttttgcc aaggttcgtc agcgccagtt
     8881 tggggtaaag cgcgttagcc acggtccgcc cctcccgtcg tcagcatggt gagcgtatcg
     8941 ccgatcttgg cgaacatctc gtcgcttgtc atattgccgc ggtaaagctg atggaacacc
     9001 acgccgtcct tgatgaacag cacacggccc gcatggctgg ccgctcgcgt ggagtgcgtc
     9061 accatcaata tggtctggcc gccgcggttg atctcgccga acatgcgcag cagctttgtg
     9121 gtcgccttgg aatccagcgc gcccgtaggc tcgtccgcca gcacaatctc cggctttgtg
     9181 ataagcgcgc gggccacggc tgcgcgctgt ttctgtccgc cggagacctc gtaagggtat
     9241 ttgtccagca gagcttcaat accgagctgc cgcgcgatgg gcgcaaggcg ggcggccatt
     9301 tcagcgtggc ccttgtgcga gagcacaagc ggaagaaaga tattgtcccg caggctgaac
     9361 gtatccagca ggttgaaatc ctggaacacg aagcccagat gctcacgccg gaactgggac
     9421 attttgctgt ccttgatttt ggtgatatcc ccgcccgaaa gacgcaccgc gccgctggtg
     9481 ggcttgtcca gcgcggcaag gatgttcagc agagtggttt ttccgctgcc gctttcgccc
     9541 atgatggcga cgtattcgcc ctcctccacg gtgaaattca cgtcccgcag ggcctctacc
     9601 tgtactgcgc cgaagcgcgc ggtatatacc ttttgtaagt gttcaacttc aagcaatggc
     9661 ataaagcagc ctcctcaaaa aatagcgccg gctgtctgcc cgcgccgttc cggat
//
LOCUS       DAWBSB010000073         9713 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1573_length_9713_cov_3.525989, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000073 DAWBSB010000000
VERSION     DAWBSB010000073.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 9713)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9713)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9713
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1573_length_9713_cov_3.525989"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1845..1854
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4799..4808
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1762 a         3209 c         2728 g         1994 t
ORIGIN      
        1 cgggactgtt tcgggctgtg tcgttggcga tgcggcagag caaccccacg acgctttctc
       61 cgggccgttt aaaaacggga attcaaagcg tttcttcctt attccagaaa agcatacatt
      121 ctccatcggc tagaacaaat ctggttgcaa cacggtatct cttcatttga tccgtctcga
      181 taacagcacg cgggttatta cgatccagcg ttgcaataac gttcctctct ctgccctgaa
      241 tttcaacaaa aaccgtccct tttgtcgtgc tcgacaaaaa ggtgaacgaa taacgccccg
      301 ccttccgaag gcaaattacc cttttgacga ctccgctgca ggatgaaaat ttcgcttttg
      361 tgaaattccg tctcattccg aaccggggcg aaccgatata aagcagcgct gtcttagcat
      421 ttattaccaa aaatccgttc agataaagga aataacccaa tccgccggcc attgccccaa
      481 gcgcaaagag cccgcacagc tttcccacga accctgctcc cttcgataaa atttcattcc
      541 cgcgcaaaga tcttcattcc agaggcggtg ttttttacgc cggcgcgggg ccgcggcagg
      601 cgcaggcctg ccgggaaggc gcgcgcattg cctccagcag ggggcaatgt ctgcgggccc
      661 ggttttgcga agcaaaacag aggccgattt aaggccgaca aggacgcggc ggaaaaccag
      721 cccttatttg ccgttgagga gcttaggggg ctttccgtgt ttacatatcc cgcccaatga
      781 cctgggcgat tttacgtccc ttttccacca cggcgcggaa tttttccggc ttcaggctct
      841 gggcaccgtc gctccaggcg gcttcggggt tgtcgtgcac ttcaatgatc aggccgtccg
      901 ccccggccgc aatggccgca aggctcatgc tttccacaag gcgccagtcg cccgtggcgt
      961 ggctggggtc gatgatgaca ggcagatggg atttctgctt gaggatgggc accaccgaaa
     1021 ggtccagcgt gttgcgggtg tatttttcaa acgtgcggat gccgcgctcg cagaaaatga
     1081 cgttttcatt gccgccggcc atgatgtact ccgcgctcat gatccattcc tcgatggtat
     1141 tggcaagccc gcgcttgagc agcacaggct ttttcatttt gcccacggcc ttcaaaagct
     1201 ggaagttctg catattgcgc gcaccgatct gcacaaggtc cacatattcc tcaaattcat
     1261 cgatcttgtc ctcgctcatc agctcgctga cgatgggcat gccggtcgct ttacgcgccg
     1321 ccgccatggc caaaatgccc tctgtgccaa ggccctggaa cgcataggga gaggtgcgcg
     1381 gcttgtacgc gccgccgcgc agcatgcagc cgcccgcgtc cttcacttcg cgggcaatgc
     1441 gcacggcctg ctcctcgctc tcgatgctgc acggcccgcc gatgacggcg atcttttcgc
     1501 tgccgcccac cttgatgccg cccacgtcga tgatgctgtc cgcctcgtga aacaggcggt
     1561 tcgccttttt gtaaggctcg gacacacgct gcacgttttc cacatacggg ttcgcccgca
     1621 cctgtttttc gtcgataacc gtcgtgtcgc ccaccaggcc gaacacattg taatccgtgc
     1681 cgcggatcat ggtgacggac aggcccttcg cctcaaactc gcggatgatc ttatccagtt
     1741 ccgccgccgg agtgccccgt tttgtgctga tgatcattgc tttctttccc ctctgcttgt
     1801 tggattaccg cgcgccgcgc ggcaggtgcg gcccagcgcc gccgnnnnnn nnnnccgttt
     1861 ccgtgcggca cgctgtagga ttattttata ctctaagtat ttttacttct gattatttca
     1921 attagttttt ccatggcgtg aacatagccc tgcttgccct cgcccgcgaa tttacagcag
     1981 caggccatgc ccagataact cacctgacgg aaatcctccc gttttgccac gtcggagata
     2041 tgcacctcgg cggcagggac ggacacagcc ttaagcgcgt ccagaatggc gatgctggta
     2101 tgcgtataag cccccgcgtt gatgacgatg ccgtccatgc gcccatacgc ctgctgaatg
     2161 gccgtaacaa ggtccccctc gtggttcgac tggtaaaact ccgcttccac gcccagccgc
     2221 cgcgcctcgg ccgcaatgta ttccaccagg ccgtcatagg tcagcgtgcc gtacaggtcc
     2281 ggttcgcgga tgcccagcat gttcaggttc ggcccgttaa tgatcaagaa tttcataaaa
     2341 acgctccttt atggcttcgg ccacgctttc aaaggggccg tcgttttcca cacgcgcatc
     2401 gcacgccgcg gcgtacagcg gcgcacgtgc cgcatgcatg gcggccagcg cctccgcact
     2461 ttgggaaagg ggccgcgccc ctcccaccgc gagctgcccg agcggcctgt cgatgtagac
     2521 caccacgccg ttctgacgca gagcgcgcac gttctgcgcc cgcagcactg cgccgccgcc
     2581 ggtggcgatc acctggccgt tttccttccc cagctccgcc gccacagcgc tttccaggtt
     2641 gcggaagggc gtttcattgc ccggggaaag gatgctgctg atgggcccgc cgatgcgttc
     2701 caccagaacg tcgtccatat ccacaaagcg cttgcccagc agcttggcgc aggcttttcc
     2761 caggctgctt ttgccgctgc cgggcatgcc caccaggacc aggttggaca gctttccgcg
     2821 cagctcccgg tcgatgcggt cgatctccgc gtccgggagc ggcctgccga gaaaatgctc
     2881 cgccgcgtac ttcgcctggg ccaccagcat cagcatgccg ttgacggcgg gcaggccgcg
     2941 ggcttcggcc tgctgcaaaa gagcggtgcg gaacggattg taaataacgt ccagcacagc
     3001 ttccagccgt gggtaattct ccagcgccac aaggcattcg ccgttgttcg gatacatgcc
     3061 cacggggctg gtgtttacga tcacctgcac gtccgcgcgc cgcgcggcct cctcgtaggt
     3121 gatggcgttc ccggtcctgc ggcggctgac gagcacggcc tgcgccgccc cctcggccct
     3181 tgccacagcc agcacggtct tctgtgtgcc gcccgtgcct agcagcatca cggtttttcc
     3241 ccgcagtgaa atgccgttcc gccgcagcag acaggtaaag cccgcaaaat cggtattgtg
     3301 gccctccagc cgtccgtttt tgttgacgat ggtgttcacc gagccgatgg cccgggccgc
     3361 gtcgtcgatg acgtcgcagt agggcatcac aagctcctta taagggatcg tgacgttgat
     3421 cgccttaaaa tcccgcgcgc gcatgaacgc gtgggcctcc tccggcgtag gcagcgggca
     3481 caggcggtaa tcgtagcccg ccagcgcctc gtggatgggc tttgaatagc tgtgccccag
     3541 ccgcccgccg atcaatccat attccatgcc gtgccctcct gccatgccgt gccgaagtgc
     3601 tggccgcaca ggtccgccag gccgaaggcc accagactgt cctgcactac acgcgcccgg
     3661 tgcaggatgc acgggtcgtg ccggccgctg atctgcagcg tggcattgcg ctttgcggca
     3721 aagtccacgg tctgctgcac cttgtaaatg ctgggcgtag gctttaccac cgtgcgcagc
     3781 accagcggca tgccgttgct gatgccgccg tttacgccgc cgttgtggtt ggtagccgtg
     3841 acgacgcggg ttccctgcat gcggaacgca tcgttggccc tgctgcccgt cagccccgca
     3901 aagccgaagc ccaggccgaa ctccacaccc ttgacggcgg gaatgctgaa cagcagcgcg
     3961 gccagcgtgc tttccacgct gccgaaaaac ggctcgccca ggcccgcggg caggcccagg
     4021 acaaccgttt ccagaatccc tcccacggaa tcgccctcgg cggcggccgc ctcaatggcc
     4081 tgcgtcatgg cgcgggcgcg cagcgggtcc agcaccgcaa aatccgcggc gttcagtgcg
     4141 tccagctgct ccttcagccg cgccgggtcg ccggaaaagg gcgcgtcgtc aatgcccgcg
     4201 cattgcgcca gatgtgtggc gatggcgacg ccctttgttt gcagcagttt tgtgaagatg
     4261 ctgcccgccg ccacaacggg ggccgtgagc cgcccgctga aatgcccgcc gccccgcgcg
     4321 tcctgatggc cgccgtactt catgtgggcg gtgtaatccg cgtggccggg gcgcagcagc
     4381 tcctctgttt ttgtataatc gccgctgcgg gtattggtgt tttcaatcac aagcgtcagc
     4441 gccgtacccg tggccgcgcc cctgtatacg cccgaaagca cgcgcaccgc gtccgcctct
     4501 gtgcgggaag tggatatttt tcccttggcg cggcgttttt ccatctgccc gcgcatgaat
     4561 tccatgtcca cgggcacgcc gggcgcaagg cccgtcactg tggcgcccac agcggggccg
     4621 tggctctcgc cgaagattgt gagcgtgacg cttttgccgt acgtgtcgct catcgcacgc
     4681 cctccccgat gatggccttc agatcctcca agggaacctt gtccagcctg cattcgccca
     4741 ggcggggcac ctttaccacg gtgatggcac cgtccggccc ggtctttttg tcgtgacgnn
     4801 nnnnnnnncg gatacgggac ggcaggccca gctttttata cacggcccgc gtgcggcggc
     4861 gcagcgaggc gtcctcgatc atgggcagca tgcccagcgc cacgcattcc ccgtggtaca
     4921 gcccgcccag atggtttgcg ctttcaatag cgtggcccag cgtatggccg aagttcagcg
     4981 tggcgcgcag gccggcctcc cgctcgtccc gctccaccac gttcttcttc atcaccaggc
     5041 tgcggtagac gatctcctcg atcttctcgt gggcgtcctc tgtttcgaac agctcaaaca
     5101 ggccctcgtc cgcgatcagc cccgccttca cggcctctgc aaggccgttg atgaaatggc
     5161 gctcgggcag gcttttcaac gtgtccgggt ccgccaccac aaggctgggc tggtaaaaag
     5221 cgcccacagt gtttttggtg cccgccaggt tgatggccgt ttttccgccg atggaggaat
     5281 cgatctgcga aagcgtcgtg gtggggcagt tgatgaacgt aacgccccgc atatagctgg
     5341 ccgcggcaaa gcccgcaagg tcccctacaa cgccgccgcc aagcgccagc accgcgtcgc
     5401 cgcgggtaaa gccgtgctcc agcatcacgg tgagcaattg gccgaagcac tccaggctct
     5461 tggtcccctc gccctgcggc agcaccacgg gcacgccctc ccggcactgg gccagtacgg
     5521 ttttcaggta tgcctccgga actccggtgt cgcttaccac cagcacgcgg cggttcaggt
     5581 tgatcagctg ccccacgcgc gaaaggccgc cctgcttgat aatgatgtca tagctgcgca
     5641 cgccaaggcg catcgtcaat ttcatactca tatcctcaca cccgcgtttt tctgcgccgc
     5701 gctgtgcgcg gctgaaaact gttttcccgc tgttttaccg tgccgcctgg cggtcatata
     5761 cgccaagcac acgcacggtc ctgcacacgc cggacagcgt gtccagcagc tctttcgatt
     5821 tttccgccga aaggtcgccc acgacctcca cataaaaata atattcccac ggcacattgg
     5881 gcatgggacg gcttttgatg cactccagat tgaagcccat cgcaccgatg agctgcatca
     5941 cacgggccag ccggcccgcc cggtggtcca cggtgaacaa caggctgaac cggttgcccg
     6001 cagcgttcat cctcctgccg atgacgatga agcgcgtggt attcgtctcg ctggtattga
     6061 tgttctccgc cagcaccgaa aggccgtaca gcgccgccgt ctccagcgaa gcgatggccg
     6121 ccacgctttt atctcccgcc tcggctacgc tttttgccgc ggcggcagta ttttggaagg
     6181 cctgcgtttc cagccccagg ctgcggatga actttgccga ctgctgcagc gcctgcggat
     6241 gggacacagc ctttttcacg tccgacaaat gcgcgcccgg aacgcccagc agattctggc
     6301 tgacgggcag gtcgtatacg tcgcacacgc agatatccct gtgggcatag cataggtcca
     6361 gcacctcgga aacgtcgccc gcatggctgt tttcaaaggg cagcacgccg taggccgcgt
     6421 cgccgccggc cacagcgtcg aacacctccg cccaggtggg atacgcctgc gcccgtacat
     6481 gctggaacag gcgggtgagg gcaatgtggc tgaacgcgcc ctccacgccc tgataggcca
     6541 cggtccccat gcccagcacc gcgcgttgga acgcgcggga caggcccatg gtgtggcgga
     6601 tgaaatcctc gtaatattca gcgtaggcct cgtccggcag ccgggcgcag tttttggcca
     6661 cgacggcctc ctcccgggaa gcgtccaaaa caggcaggcc ccgggcctgc ttgtatgcgg
     6721 ccaccctgcg cacggcgtcc atacggcggc agaacagcgc cgccatctcc gcgtccaccg
     6781 cgtcgatctc tctgcgcgcc tgttccagct cgttttcctg ctgctccatg ggcagcacct
     6841 ccccgttgct tcgatttttc gggcaaaatc caggtaaaaa cggatataaa atgtccggcg
     6901 tcctgcaacc cgtaaagcgt atatacagat gtattatagc attatccgcc cggcagcgct
     6961 atacaaacca cacaaaaaac aagattttat cccggcaatt cggcaatttt tcaaacggcc
     7021 agggcggcgc cgccgaccga tatgctataa tgatctcaaa cccacgcgat catgaaaaag
     7081 ggggaacgga aagaatgaag attcgtcttg caaaacacac ccgcgccctt gcggcggcgc
     7141 tggcgttctg tgtgctcagc gcggtgccgg cttacgcgca ggaggacgac acagtggagg
     7201 ttcccggcgt gcccgtcacg ctgtcgctgc ccgccggcag cctcatcctt acgcgcgaaa
     7261 cacccgtgga cgacgccatt ttcgaaacgt tgaacgtaga tggcgcagcc atactcgaaa
     7321 acatgaataa aaacgatctg tatctggacg ccgttctttt taaacctcct ttggagttta
     7381 cccttgtccg cgttcaactc ccttccaaca gcctgatcga ttgggaaaac tccgaggatg
     7441 cgcaattgtg cgcttttgta gaatccatct acgaacaact ggataatatt acggccgacg
     7501 attgtagcgt ttactcctct gaacagggca tccgctttgt ccgcgccaat attctaggtt
     7561 ccaaggatgg cataacaacg cattcctgtc aatacatcac cattcgcggt gccgatgtct
     7621 attacttaac aggaattgcc ctgtacggtg acacgctcac ttcttcggaa tacgattttg
     7681 ttgagaaagt cgccggcggc ctgcgcttta ccggcccggc cccacttccc cggacggctt
     7741 ccgccgggct aaatggggac gacccgctct acaaagtgct ggtcggcgtg ctgtcggcag
     7801 cctgtctggg gctcatcgca tgggtaatcc gtaacatgcg caaaaaaagc gctccttgcg
     7861 tcccggcgca ggccatgccg ccggcggccg agccctttgg ggctaccacg gtgggagtcc
     7921 ccccggttcc caccgcttcg gccgggcctc cgggcgccga gcgctcggga gccgcgcccg
     7981 acgctgtccc cgtcttctgc ccgctgtgcg gccagcgcct gccgtccggc gtaacgttct
     8041 gtcccggctg cggaacccca atttcctgac gcccgtactt catgcatctt gcaagcgccc
     8101 ccgcgcggcc gttttttacg ggccgcgcgg gggcgctttt gctttatctc aacggccctg
     8161 cagcatgggc atctgcgcca tgcggccgaa ggcctcctcc attttgtcca accccacggt
     8221 gcaggcgatg cgcagatacc cttctcccgc gtcgccgaag gccgagccgg gcagcaccag
     8281 cacatgggct tcttccaaaa tgcgctcaca ggcctcggcg cttgtaagcc ctgtcggccc
     8341 cacaccgggg aacaggtaaa tgccgccccc cgccgggctg agcgtgaacc ccggcagctc
     8401 ccggatgcgt tgtgcgccat aggccatgcg cgcggaaaat tccggcacga tctgcgcagc
     8461 aagctctttg cggcggcgca gcgcgtgcag cgcggcgcgc tgggagggcg cgggcgcgga
     8521 atataccacg ctgtcgttga tgcttttcac cgcccgcgca atgggctccg gcgctacaat
     8581 gttgcccacg cggaagcccg tcatcacaaa attcttgctg aagctgttga tggccaccgt
     8641 gcgctctttc atgcccggca gcgtcaaaac aggcaaaaat ggctcgctgt aacaaaagct
     8701 ggtgtaaata tcatccgcca ccaccagcag gtcgttcttc cgggcaaagc gcgccaccgc
     8761 ctccagcgtc ggtcgggaat aacacacacc cgtgggattg ttgggtgtat tgatgaccag
     8821 cgccttggtg cgcggcgtca gatactgcgc agcgcgctcc atatccggct gccagccgtc
     8881 cgcttcgcgg cagggcacga acaccggcac gccgccgccc agccgtacct gtgtggcata
     8941 ggctgaaaag tacgggtcaa aaatgagcac ctcgtcgccg gggtccagga tggcctccat
     9001 aacaaggtac atggcaacgc acccggccgt ggagacaaaa atttcccgtt cacccagcgc
     9061 cacgccgtat tcttcggcgt aaaaccgggc aatctcctcc cgaagctccg ggtcccccca
     9121 ggggtcggtg tatttcgtat ggcccgcgcc cgcgtctgca aacgccaggt cgatgatttt
     9181 ctgcggcgtt ttcaggtccg gatcgccgat ggacaggtcg atgatatcag gatatctgcc
     9241 cgccttctca aatactgtca tcatggggtt gcggtcttcc tgctgccaac gccgcgctct
     9301 gaaatctttc atgctctctc cactcctatt ccccgggggt ccggccgtcc gccgggccaa
     9361 tataggcgaa ccgccgagac gctttgccgt cccgttccac cgtttcattc cacatagcgg
     9421 ccggacgcac ccaaagcccg cgctcaccat acagcgcacg gtataccacc atttcctctt
     9481 gcgtctcgct gtggcgggca cagaacagca cctcatactc gtttcccttg aagtgccggt
     9541 aacggcccgg ttttacggat ggctccatat ccgtccctcc cttttttgcg ctgaagatat
     9601 gccaaaagta tagcacaaag ccgccgcccg tgccattctt tttgcgttca cagcaggaat
     9661 atggtataat atgtccgctt ggcgaatgcg agccacaggc gaagcatgag cct
//
LOCUS       DAWBSB010000074         9592 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1589_length_9592_cov_3.158436, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000074 DAWBSB010000000
VERSION     DAWBSB010000074.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 9592)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9592)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9592
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1589_length_9592_cov_3.158436"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1282..1291
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7244..7253
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2003 a         2598 c         3051 g         1920 t
ORIGIN      
        1 gtatatttgc cctcgcccgt caggttgccg tactcccagg agccgttctg gaagaacacg
       61 gcctcgccgg ccaaaaactc attgcgggag tcgtcgccgg ttttggcgga cagctcactg
      121 ggctcgcagg tgctgttgtt gatatacagg tcccagatgg ccttgtaatt gtccagatat
      181 gtgcccttga tggcgtcggt ggtgctgatg ccatcttcct gatattcaaa gtaaacgggc
      241 aggttggcca gatgggtctt gaagcgccag tcggagctgc cgtccatgcc cgcgctggta
      301 aacgcggcaa agcccagctc gtccttgcgg gaggtaatat cctcggcaac tttcttcaga
      361 tcctcaaagc tctggatgtc ctcgagggta tagccggctt tctccaacag ggtcttgttg
      421 gtgatgatgc cgtagctctc gatgacgtac gcgatgccgt agacggtgtc gccttctttc
      481 agcgcatagt catcgctggt gagctgggtg tagatgtcgg agccggaaag gtcatagcag
      541 taatccttcc agttggcgag gccgacgggg ccgttcacct ggaacagcgt cggggcttcg
      601 cttttgccca tctcggccat aagcgtcgtt tcgtattcgc cggaggcggc cgtcacgacg
      661 gttacgggaa cgcccgtttc cgcggtgtag gctttggcga gcgcctgcca ggcctcgtcc
      721 tgctcaggct tgaagttcag gtaataaaca gaacctgcgg ccgtgggggt ggtgctggcg
      781 gcgggttcgc tgccggagcc cgaagccgcg ggcatagagc tgctcgagcc ggagccgccg
      841 caggcggcaa gggaaagagc caggcaaagc gcaagtacaa gtgcaagtac ctttttcata
      901 ttggtcctcc taaaaaaatt tatcattcaa cggcagaact gatcgtctct aaacaatgca
      961 tttgcgctta cttcggcaga tgcggcttta aagacggtat gaagctgccg tcaaatatca
     1021 gatgttacgg aggcgacaga ttcgccctcc agaagcccgg tgctcaccac gcgcgtctgc
     1081 ggaggagcag ccttttcgat ctgtcccagc agaagggcca cgctctggtc ggcaatctcc
     1141 tgctgcggct gggataccgt ggccagccgc ggtaccgcgt actgccccag ctcgatgccg
     1201 tcgaagccca ggacggagat atcctgcgga acgcggagcc ccgcatctgt gagggcgcgc
     1261 atggcgccca cagccatgat annnnnnnnn ngaacacggc ggtgaagccc gcgtcccggg
     1321 ccagcagctt gttcatggca cgatatccgc tgccgagatt gtaattcgcc ttttgataca
     1381 gcgcctcatc gtgggaaagc ccctcgtctg caaagccctg caggaacccc tggtagcgtt
     1441 ggtcgctgat ggaggagacc gcccggttgc cgccgataac ggcaatgcgc cggtgcccgc
     1501 gggaaagcag aaaacgggcc gcgcgccgtc cggcggcggt atcgtcgatg cccacgctgg
     1561 acaggtttcc aaagttcagg tgatctgcaa gtgtggtggc cagcacgctg ggcacggcaa
     1621 tggcggaaaa gcgtgcctca aagctgcagg tgttgccgcc caggaaaatg aaccccagag
     1681 gtttgcgctc gcgctgaagc tgctcgccca ccagcacctc atcggcgtcc tcgtccaggt
     1741 aatggacctc ggcgctgtac ccggagcagg aaataaggct ctgcatccgt tccagaatgg
     1801 cggcgaaaaa catgttgaac gcgcccttca ccacaatgat gatgctgcgg ttctgctgta
     1861 ccttcaactg gcgggcattg ctgttgggca cgaatttatg cgccgcgacg atggccttga
     1921 tcttttcctt ggcctcacgg gaaacgtcag gatggtcgtt cagcgcgcgg gaaacggtgg
     1981 atacggcgta cccggattcc cttgcgatgt ccttgatcgt caacacctgc atcccccttt
     2041 tttgaaaggc tgcaaccgtt atcgggaacg ctcccggaaa cgtttccagc tccttctgtt
     2101 gcttatcata cacctacgcg tgaggaattt gcaagagggt tttggcaatt tcggcaattt
     2161 gatttaattg aactattgtt tattgtgcac ctttacggca taaaacaatc ggcctgtcat
     2221 tttgttgtaa tcatttgcgg gccggccgct gtacaaacaa aaaatgagaa aagattcaca
     2281 ttcttgtaat ccttgtgcgg gtttgatata gtataataga tgtgctgtga aagcggcgcc
     2341 tggatagtgt accgggaatc cggtgcaaaa aagcgcgttt tctgcggtga aaatttaccc
     2401 gtatgcgggg aaagagcggt tgatatggga tttttttcaa acatgtatac caaagagggg
     2461 ccgggcgtgc gcaaggatca gccgcccaaa aaggggattc cgcgcttttt tgaaatactg
     2521 atgcgcgact acggccatct gctgaaggtg aattttctgt ttcttttgtg ctgcctgccc
     2581 atggtgacgg tggtgctgtt cggcctgctg ttccaccagt atctgggcat gctgctcatt
     2641 gcggccgtgc tgtatctgct gtgtgcggtg ctggtgggcc cggccatgac gtgcctgcac
     2701 ggcatcaccg taaaaacagt gcgggatgag ccctgctata tgtggcatga attcaaaaaa
     2761 tgctggaagg gcaactggaa gcaaagcgtg cccgcgggtg tcctgttctg cacgctgctg
     2821 gcaatggagt gcatcgcggc ctggtattat atgttcatgc agtcggagat gaacgtgctg
     2881 ctggtggcat tcgtggcgtt cagcctgctg ctgctcacga tctgctggct gttcacgacc
     2941 ctgcagatgc tgtatctcga catgcccctg ggcggtatgc tcaagaacag cctgctcatt
     3001 atgttcggct acgccaaacg cacgctgccc gcagggctga ttacgctggt catcgtcatc
     3061 ggggtggtgc tgttcgtgcc cgttcccatc atgcttctgc tgctgttcct gggaatgcct
     3121 gcactggtgg ccgtcatcgg ggatatgtgg gcctggcccg tgatggagca ggcgttccac
     3181 atctctgagc agcaggcggc gcgccgtgcc gagcaggagg gccgggagtg aacctgagcg
     3241 tgcatcatgt ggcggtcatc gtttcggatt acgctgcggc aaagcatttt tatgtggatc
     3301 ttctggggct gcccgtcgtg cgtgaaaact gccggggcaa cggcctgtgg aagctggacc
     3361 tgcgcctggg cgacggcgaa ctggagatat tcaacgccca cgagccgccc gcgcggctgt
     3421 cgtacccgga ggccgcgggt ctgcgccatc tggctttccg tgtggacagc gtggctgagg
     3481 cggttgcaga gctggctgca aagggcattg cgtgtgagcc ggttcgcatg gatgaatatt
     3541 cccgcaagcc gtttaccttt tttcgtgacc cggacggcct gccgctggag ctgcacgagt
     3601 gacgcgctgc gctggaagtt tctattgata ctgtatgaag ccgcacacgg caggacaccc
     3661 tgtctgtgtg cggccgtttt ttgctccgca aaacttgaaa aacaatttat ataaatctgt
     3721 tgacaaatta ttatatctga tatacaataa atttacactc aaagcatgga ggtgcaggac
     3781 cttgagcaaa agcgtataca gtgttgtgct gatggacgat gtggtggacg ctgtggaccg
     3841 cctggcctat gaagccggca cgaaccgctc gaatatgatt aaccgcattt tggcggaata
     3901 tgtgcagctg gctacgccgg agcagcgcat gcaggacatt ttttcttcca tcatggatgc
     3961 tgtcagcggg cagaatgcgc ttcagcttat gctgaatgcg tcggacgcga tgctctcgat
     4021 gcgcagcgcc atccattata aatacaaccc cagcatgcgc tacgtggtgg agctgtaccc
     4081 ccattccaac gaggagctgg gcgaggtgcg cgcgggcctg cgcacacaga acccttcgct
     4141 gctgctgtac atcggccagt tttataagct gtgggccggg ttggagcgcg cgtatctgga
     4201 aggcccgccg cgccgcagcg aggcgcaggg agggcggtat acccgccgcc tgcttctgcc
     4261 ccggaatatc gccgccagcg aggaggctgg ccgcgctatt gcgggttatg tggcgctgtt
     4321 cgacgcctgc ctgaaaactt tttttgacca tttggaggac cccgccggcg cgccccgcgc
     4381 cgtagagggg tgttaccgct ccggcctgga cgcagagact gcggccctgt agccgcctgc
     4441 gggccggaac gttcatcggg cagtgccgtg cgccgcacgg ctctgccccg tagattcatc
     4501 cgacaaaata tgcggataag gagtgaaatg atatgaatgc acagaaaatg acacaaaaat
     4561 ccctcgaggc attacaggct gcacagtcgc tggccgtcga gtatgaaaac caggccctgt
     4621 gcccggagca cctgttctgc gctttgctga gccagcagga cgggctcatc ccccagctgt
     4681 ttcagaagat gggggccgag ccgggcaacc ttgccgccgc ctttgccaaa aaggtgggtg
     4741 agctgcccca tgtaacaggc accgggcggg accccgaaaa ggtgtatatc acgccggagc
     4801 tggaccgtgc gctgacggcc gccgaaaaat ccgctgccgc catgaaggac gaatacgttt
     4861 ccgtggagca tcttgtgctg ggcatgatgg atgcgcccaa cgccgccgtc aaggaggtgc
     4921 tgcgccagtt cagccttgat aagacccgct ttctggaggt gctggcgcag gtgcgcggca
     4981 accagcgcgt caccagcgac aaccccgaaa gcacctatga cgtgctggcc aaatacggcc
     5041 aggaccttgt ggagctggcc cgtcagcaga agctggaccc cgtcatcggc cgcgacgccg
     5101 agatccgcaa cgtcatccgt atcctttcgc gcaagacaaa aaacaaccct gtgctcatcg
     5161 gcgagccggg cgtgggcaaa acagctatcg ccgagggcct ggccctgcgt atcgtgcgcg
     5221 gcgacgtacc cgaaaacctg aaggaccgca aggtgttcag ccttgacatg ggcgcgctgg
     5281 tggccggcgc aaaataccgc ggcgagtttg aagaacgcct caagagcgtt ctgcaggaag
     5341 tgaaaaagag cgagggacag atcatcctgt ttatcgacga gctgcacacc attgtgggcg
     5401 cgggcaaaac ggacggcgcc atggacgcgg gcaatatcct caagcccatg ctggcgcggg
     5461 gcgagctgca ctgcatcggc gccacgacgc tgaacgagta ccggcagtac attgaaaagg
     5521 acgcggcgct ggagcgccgg ttccagccgg tgatggtgga tgaacccacg gtagaggaca
     5581 ccatctccat tctgcgcggc ctgaaagagc ggtacgaggt gtttcacggc gtgaagatcc
     5641 aggacaacgc gctcatcgca gcggcagtgc tctcgggccg gtatattact gaccgctttc
     5701 tgccggataa agccatcgac ctggtggatg aagcctgcgc catgatccgc acggaaatgg
     5761 attccgcccc ggcggagatg gatgacctgc gccggaagat catgcagcac gagatcgagg
     5821 aggcggccct gaaaaaggag accgacaagc tttccgccga gcgccttgcg gacattcaaa
     5881 aagagctggc cgccatgcgc gaggaattca gcgccatgag cgcccgctgg gagaacgaga
     5941 aatcggccat cggcaaggtg cagcacctgc gcgaggagat cgagcaggta aacggcgaga
     6001 tcgccagggc cgagcgtgaa tatgacctga accgcgctgc cgagctgaaa tacggccgtc
     6061 tgcccgcgct gcaaaaggag ctggagcagg aggaggcccg cgcggcggcg gcggaaaagg
     6121 attccactct gctgcacgac cgtgtgacgg aggaggagat cgcccgcatc gtgggccgct
     6181 ggacaggcat ccccgtggcg aagctcatgg aaggggagcg cgaaaagctg ctgcatctgg
     6241 acggcatcct gcacaagcgg gtcatcggcc aggacgaagc cgtacagcgc gtgacggagg
     6301 ccatcctgcg cagccgcgcc ggcatcgcgg acgaaaaccg ccccattggc agctttttgt
     6361 tcctgggccc gacgggcgtg ggcaaaacgg agcttgccaa agcactggcg gaagcgctgt
     6421 tcgatgacga aaagaacatc gtgcgcatcg acatgagcga atatatggag aaatattccg
     6481 tcagccgcct gatcggcgcg cctccgggat atgtgggtta tgaggaaggc ggccagctca
     6541 ccgaggctgt gcgccgcaag ccgtacagcg tggtgctgtt cgatgaaatc gaaaaagccc
     6601 atccggatgt gttcaatgta ttactgcaag tattggatga tggacgtatt acggacagtc
     6661 aaggccggac ggtggacttt aaaaatacca tcatcatcct gacgtcgaat ctgggctcca
     6721 actatatcct ggacggcatc gaaaacggag agatcagccc cgaggcccgc gcccaggtgg
     6781 acgctctgct gaaaacgtcc ttccgccccg agttcctgaa ccgtctggac gagatcgtct
     6841 attacaagcc gctttccaag acggaaatcg caggcgtggt ggacctgatg ctggaaagcc
     6901 tggtgcagcg cctggccgcc aagcagctca ggctggcggt cacggacgcc gcgaagcaga
     6961 tgatcgtgga cggcgggtac gacccggtgt acggcgcccg cccgctgaag cgttacattc
     7021 agcagaaggt ggagacgctg gtggcgcggg atatcataca aaacgatccg gcgcccggag
     7081 ataccatcac ggtggacgtg aaggacggcg ctctggcgtt ggtgtaagcg caaaagacgc
     7141 atccgcaggc cggacatttt gcccggcctg cttttttgcg tgccggggcc cggacgaaaa
     7201 cctttctccg cactggattt ttcagttcgg aactgctata atgnnnnnnn nnnggcggcc
     7261 tgtgaaccgg cggaaaaagg gcggctttgc cgcgggctgc tgcgttgcgc tgctgctgat
     7321 cacgctgtgc gcggcagcgg gcggcggcgt actgtggtat gtggggcgca cgatggcaca
     7381 gccgggcagc gcgtccatgc agggcgcttc cggtgcgccg ggcggcggag cggaggatgc
     7441 cgggcggcag gagccgctgt tccgggaggg agacacggtg gatacggtcc ttgcaaaaat
     7501 gacgcttgcg gaaaaggtgg gccagatgtt catcgtccgc tgcccggaac agggagcggc
     7561 ggagaaggct gcggaatacc atttgggcgg ctatatcctg ttccgggaaa attttgagga
     7621 aaaaacaccc gtgcaggcgg cggaaatggt ccgggcctgt caggctgccg ccgcgctgcc
     7681 gatgctggtg ggtgtggatg aagagggtgg gaccgtcaac cgtatcagcg gatatccggc
     7741 tttccgcgac acgccctttg cttcgccgca ggagctgtac caggcgggcg gctttgacgc
     7801 ggtgcgggaa gatgcgctgg aaaaatgcgc gtttcttgaa gaactcggtg tcaacgtgaa
     7861 ttttgcgccg gtgtgcgacg taagcacgga cccggaggat ttcatctaca aaaggagctt
     7921 tggccggaac gcggcagaga cggcgcagta tgtgcgcacc gtggtggaga cgatgggtga
     7981 gcgcggcatg ggaagcgtgc tgaaacactt tcccggctac ggcaataacg ccgacacgca
     8041 cacgggcatt gccgtagacg accgccccta tgacagcttt gcagcgtcgg actttctgcc
     8101 tttcagggcc ggaatcgaag caggagcaga cattgtcctt gtttcccaca acatcgtcag
     8161 ctgcatggac agcgcatatc ccgcctcgct ttcaccccgt gtccatgaaa ttttgcggga
     8221 agaactgggc tttacgggcg tgaccgtcac cgacgacctt tacatggacg gcatccggga
     8281 ctttgtggac gtggagggcg cggcggtgca ggctgtgctt gcgggcaacg acctgttgtg
     8341 ctgtacggat tttgaggtac agatcccggc ggtattggcg gcggtggagc agggagtggt
     8401 gcctgagaca cgagttgatg aagccgtgcg ccgcattctg acgctcaagc agaagctggg
     8461 gctgctgggg taaggcggat ggatgcggca atgaccgccc aaaaagaaaa agcgcggcct
     8521 gtccacccct caaaggggaa ggacgggctg cgctttttgt gcagcgttgc gtgtatttcg
     8581 gtatggattt gggatatttg ccgacatttt tgaacaaaaa gccgtttgga tgccgctggg
     8641 gatgaatata atggagggga taaagcgggc gttcttcgga atgccccaaa aataaaagga
     8701 ggactggata tcatgcaggt acgttgggaa tatttgactc cgcccgaatt caaaaagctt
     8761 gttaaagagg agcagatttg tattttgccg ctggggatac tggagcgcca cggcgaacac
     8821 ctgccgtatg gcaccgacgg cctctcggta cacgctattg cctgccgcgc ggcggaactg
     8881 gagccgtgtg tggtgttccc cacttattgg ttcggccaag ggcacgaggc gtcatgcttt
     8941 gcgggctccg tcaactttcc cccccggctg ctgtacgaga tgctggaagt gctgttggac
     9001 cagatcgccc gcaacggctt taaaaaaatc gtgattttca gcggccacgg cggaaacagc
     9061 cattttctgg actactttgc catgacgcag ctggaccggg aggtggacta cacgttgtat
     9121 attctgcgcg gcgcaggcaa acgacagctg gctctggccg atatgtggga gacgaagtac
     9181 ggccatgcag gtgaagaaga gaccagccag atcatggcca tcacgccgga gggcgttgta
     9241 aagctggagc agcaggtcta tgaggaaccg atactcccgc gcatggacct gacgcagcag
     9301 gtgcctgggg tacattcggg cctgtggtgg tatgcgaaat atcccctcca tgtaacagaa
     9361 agcccatccc acgcgacaaa ggaaaagggc gaacgtatga tagaggctcg cgtgctggat
     9421 ctggccgacc gtttgcgcgc cattaaggaa gataaggtgc tgccctctct gcagcaggag
     9481 ttttacgagc gtgtgcgcag ggtgccggaa ggagattgaa cccgcccggc gaacagagcc
     9541 ggaagaaagc ctccataaat aaggaaggaa aagaagcgtc cggggaaaaa gc
//
LOCUS       DAWBSB010000075         9504 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1600_length_9504_cov_3.172823, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000075 DAWBSB010000000
VERSION     DAWBSB010000075.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 9504)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9504)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9504
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1600_length_9504_cov_3.172823"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1838 a         2850 c         2883 g         1933 t
ORIGIN      
        1 atgttgatgt agtcgaacag attggtgtcc atgaaatagc ggtagctctg gtcatgcgtg
       61 ccggggatac cgcagtattc atccaggttc acggtgtgca cgcgggagaa atctacctcg
      121 ccggccttat tcatctcaat gagctttttg tagatgggca ccggcgtacc gcccgtggca
      181 aggccgagct tggcatcggg cttggcgttt acaagttcct tgatcatatt ggcgatgacc
      241 gcgcaggtct cgtcatagct gttcgttacg ataactttca tggaagtcgc tcctttttct
      301 ttattttaaa ggcttcttta cttttaatca tagcgttttt tgcgtggggt taaaaggcgt
      361 aaaaagcctt taattttgca caaacgtgcg cggcgctgca aaatgcggcc ctctcaaatg
      421 tacagcgcgc tttccagctc gttcaccgcg cctccgcgcg ccgcgcccgc gttctgcgcg
      481 cggaaagcgg cgggcgtttg cccggtgtgc tttttgaaca ccttgcacag gtagttcgcg
      541 cccgaaaagc cgcacagcgt ggccactact tccagcgggt aataccgccg ggccaaaagc
      601 gccttggcgg cgtccagccg gacacccgtc aaatactgcc ccgggcccac gcccatttca
      661 gccgaaaaca cccgcaccag atggcttttg ctcacgccca gctgggcgct cagctcctcc
      721 acgccgtaca ggcctgcata attctggcgg atggccagca ccgcatccgc caccagcggc
      781 gaaagccggg gaacggccgc gtcggcggcg gcaagttcgc acagaatatg gtagcacagc
      841 gcggaaagcc cggccgcgcc gccgcgttcc atggcggcgg caagctcgcc cagcagctgg
      901 gcggccatgg ggcaggcgct gcagtcgctg gcaagcgggg cgggcaggcc cgcggctgcg
      961 gcctgtgcag ctgtgccgga aaaaccagcc gccagcacgt ggcagccggt cacgggcgtt
     1021 acctcaacag ccacgcctcc cgcaaggaac agcccgcccg ccgccagaag cacgggctcg
     1081 ctgtccgcgg aagcggccgc gcgcccgctg ctggccagca ccaccgtaaa aggcgcggcg
     1141 ggcagcagca caggcgtttc gcctgtgacc tcccggcaga acaccggcgt gcagacggcg
     1201 ctttcgtcaa atatcaacaa tatccactcc gtatcaatat tttactattt aaaacagcgt
     1261 ttccagctga tattattata ttaaagacgg aaccaaccgt caaggatatc aacaaaatcc
     1321 aaaattattg aagaatacgg aggctgattc ctatggcaag cattagcttg aaaggcatct
     1381 acaagatcta tccgggcgac gtgaccgccg tttccgattt caaccttgag atcgaagaca
     1441 aagagtttat cattctcgtc ggcccttccg gctgcggtaa atccaccacg ctgcccatga
     1501 ttgccggcct ggaggagatc tccaagggcg agttgtacat cggtgaccgc ctggtgaatg
     1561 acgttccccc gaaggaccgc gatatcgcga tggtgttcca gaactatgct ctgtatccgc
     1621 acatgacggt ttacaagaac atggctttcg gccttgagct gcgcaagacc ccgaaggacg
     1681 aaatcgacaa gcgcgtgcgt gaagccgccc gcgtgctaga catcgagcat ctgctcgacc
     1741 gcaagccgaa ggctttgtcc ggcggccagc gccagcgtgt tgccctgggc cgcgccatgg
     1801 tgcgtaaccc ggccgtgttc ctgctcgacg agcccctttc caacttggac gccaagctgc
     1861 gtacctcgat gcgcaccgag atcatcaagc tgcaccagaa gctgggcacc acgttcatct
     1921 acgttacgca tgaccagacc gaggctatga ccatgggcga ccgcatcgtg gttatgaagg
     1981 acggcctgat ccagcaggtg gacactccgc agaacctgta cgattatccg tgcaatatgt
     2041 tcgtcggcgg cttcatcggc agcccgcaga tgaacttcct ggacgctact ctggtgaagg
     2101 aaggctccaa ctactatgtt gacctgggcg gcgacaagct gctcatcgct ccggagaagg
     2161 ttacttccgc gctggatgct tatgtcggca aggcgatcaa ggccggtatc cgtccggagg
     2221 acatcaagga cgacgaggag ttcatggaga agcacaagga cgccaccatc accgcgcatg
     2281 tggaagtttc cgagctgatg ggttctgaga tctacctgta cctggagtac aaggattaca
     2341 agatgacggc ccgcgtgaac ccgggcagca gcgcgaagaa cggcaccgat gtgaaggtcg
     2401 ccatcaacac caagaagctg cacctgttcg acccggagac cgaactggcc atcctgaact
     2461 aagtagtaca gagcaataac agcaaccaaa gccccggcgc ttttgcgccg gggctttttt
     2521 tgtacacaaa aaagccgggc ggagtctttc cccgcccggc ttcgccgatt attttttctc
     2581 ttcctttttc gcgtccagca gggatttctc caaccagatg gtgccgatct ccagcgcgct
     2641 ccacaggccg tttttatcga tctgcgtctt catgcgctcg atggggcttt tggtggtgtc
     2701 ggtggacagc agcgtaacgt ggatgcggtc cgccacctgc acgccgtttt cttccttgct
     2761 ggtgagcacc tgcatgaaga tgacgtacgg gtccgacagg ctgccgtaat agatgtcgtt
     2821 tttgcagcgc accagcggac ggcccttata tgtgagtttg ggtgtaaaag ccatgaaacg
     2881 ggtctccttt taagattatt cgccgaggta acttttcagc agcgcctgca gcagatcgtc
     2941 gcggtcgatc ttgcggatta gggagcgggc gccctgataa gtagtgatat aggtgggatc
     3001 ttccgagagg atgtacccca caagctggtt gatggggttg tagcctttct ccttcagggc
     3061 gcggtaaacc tccgtcagca cggcgcggat ctcgcggtcc ttatcatcgt aaatggagaa
     3121 gatggtggtc ggttcgctca tactctcatt ccctcctaca aaagcgttcg gtatcaatct
     3181 atattgtagc ctaaaagagc cgccgatgca agcatttggg gcaaaaacgc cgcaatcggt
     3241 atttggggct gccggcaaag cagcgcctga gaagcattgg cactgggctt accggcagcc
     3301 ccattccggt tcgaacccca ttttcagctg cgcagctcca cgccgatttc cgccaggttc
     3361 gccagtacct ttttcacggc gttttcggct tcttcgtcgg tgagcgtgcg gtccgccgcg
     3421 cgcagaacca gactgtacgc cagactcttt ttgcccggag ccatatgctc gccggtgtat
     3481 acgtcgaaca gcgtcaggct ttccagcgct tttccggcag ccttacggat gcgcgacgcg
     3541 atgtctgccg cgggcacggc gtcgtccgcc accagcgcaa ggtcacgggt aatggccggg
     3601 tgcttgggca gctgcttgaa cgcgggggtg ccgccgcggt gagcgaacag aacgtccatg
     3661 cgcagctcgg cggccacaac gcgcggcttt acgccgtagt tggccagcac ggcggggtgt
     3721 acctcgccaa tggttcccag cttatcttcg cccagccaga tatccgcgca gcggcccgga
     3781 tgatacgatg tgcccgccgt gttgcgcgca aagcgcgcgt ctgcaacgcg ggccgtttcc
     3841 agcagctttt ccaccacgcc cttcaggccg agataatccc agcccgcgcc gtaggcgccc
     3901 agcatcaggg tttccgtctc ggtggggagc tggtccgggt cggcgttggg ggtataggtg
     3961 gtggtgtcct caaacagcgc gcactcggcg gcgcgggcgt tgcagttgcg cgcgattaca
     4021 tccatcatgc tgggcagcgc cgtggtgcgc atgatgctgg tgtcctcgcc cagagggttc
     4081 gagatgacca gcgcgttgcg cagtgggtcg ttttccggca gattgacggc gtcgaaggcc
     4141 tttcggctgt aaaagctgaa ggtttcgatc tcgtacatgc cgtagcccac cagcgcgttc
     4201 atcagcgttt catggaaggt ctggcgctcg gtggggcggg cgctggccac gccccggatg
     4261 gccgtgctgg gcattttgtt gtagcccaca aaacgggcaa cctcttcggc cacgtcgcag
     4321 tcccgctcca tatcccagcg gctggaaggg atgacaatat cgtcgccgtc catcccgaag
     4381 cccagcggca gcaggatatt taccatctgc gctttgtcca gctgcgtgcc cagcaggcgg
     4441 ttcaccgcat ccacgcgcaa ggggatgcgg cgttcttcgc gcttgtgcac ccagacgtcc
     4501 accacgccgt tcaccacgtc gcccgcgtcc agaagctgca ccagctccag cgcacggtcc
     4561 aggcagcggc ggcagccgtt ggggtccagc cctttttcat agcggccgct ggcctcggtg
     4621 cgcatgccca gctttttggc cgtggtgcgc acggaggggc cgttgaacat agcgctttcg
     4681 aacacgatgg tggtagtgtc gtcatacacg ccggaaaact cgccgcccat gacacccgcc
     4741 acggcgatgg ggcctttttc gtcggcgatg acgagcattt cctcacccag cgtgcggttt
     4801 gcaccgtcca gcgtttcaat ttcctcgccg ggcttcgcgt tgcgcacggt aatgtgcccg
     4861 ccgtttacat atttatagtc gaagcagtgc atgggctggc cgtattccag catgacatag
     4921 ttggtgatgt ccaccaggtt gttgatgggg cgcacgccgc acaggcgcag gcgctcgcgc
     4981 atccagcggg ggctgggctt cacgcgcacg ttttccacca ctgcgcccac ataacggtag
     5041 cacagtccgg ggttttcaat gtctaccttc aaaaagcggt tcacatcgcc gtggccgggt
     5101 tttacctgcg gctcgtggtc cagaaagggc acgccgaagg tagcggcggc ctcccgcgcc
     5161 agaccgcgca cggccagaca gtcgggccgg ttgggcgtga tctcaaaatc cacgcacacg
     5221 tcgtccatac caagcgcctt cacagcgtct gtgcccacgg gccattcctc gtccagcacc
     5281 agaatgccgt cctcgacggc gtttggaaaa tcgtgtgtag taaggcccag ctcggacagg
     5341 ctgcacagca tgccgcccga ttccacaccg cgcagcttac ccctgcggat ctccttgccg
     5401 ccgggcaact ttgccacgtg cagtgcggcg ggcacaaggt cgcccacctt caggttgttt
     5461 gcgcctgtga cgatctgtac cggcgcatcg cggcccacgt ccacctggca cactaccata
     5521 tggtcggaat ccggatgggg gacgatctcc aaaattttgc ccacgacgac gttttttatg
     5581 ccgtcggcct cgcactcgta ggtttccacc ttgctgccgc tcatggtcat gcggtcggca
     5641 aattcctggg gagtgcattc aaattcggcg aactctttcg cccagttcat cggcattttc
     5701 atggtctata cactcctttg ttcagaattg tttgagaaaa cgcaggtcgt tttcgtacag
     5761 caggcgcata tcgtcgatgc cgtagcgcat catggcgatg cgctccagcc ccacgccgaa
     5821 ggcgaagccg gaatacacct cggggtcgat gccgcagttt tccagcacct gcgggtgcac
     5881 catgccgcag ccgaggatct cgatccagcc ctcgcccttg cacatgctgc agcccttgcc
     5941 gccgcactta aagcagctca tgtccatttc ggcggacggc tcggtgtagg ggaagtggtg
     6001 cgggcggaag cgcacgcgcg tttcttcgcc gtacagcttt ttgatgaaaa gctccagcgt
     6061 gcctttcaaa tcgctgaacg tcacgccctt atccaccaca aggccttcgc actggtggaa
     6121 aatggggctg tgtgtggcgt ccaccgcgtc ggagcggtaa acgcggcccg ggcagatgat
     6181 gcggatgggc ggcttctgct tttccatcgt gcgaatctgc atggggctgg tctgcgtgcg
     6241 cagcagcacg ttttcggtga aatagaacgt atcctgggtg tcccgggccg ggtggctctt
     6301 gggcatattg agcatttcaa aattgtagtg gtccagctcc acctcggggc cggaaacgat
     6361 gtcgaagccc atgcccagaa aaatttcctt cagctcgccc agcacctgct cgaaagggtg
     6421 gcgcttgccc tgcgcgggcg ctttgcccgg cagcgttacg tcgatttcct ccgcggccag
     6481 gcgcgcggcg agcgccgcgc tttccagctc tttggtgcgg cgcgcgatat cggctgtgat
     6541 ggcctcgcgc acctcgttgg ccagcgcgcc gacgacgggc cgttcctcgg cggaaagccg
     6601 gcccatctgc tttaaaatgg ccgtcagctc gcccttcttg cccagaaagc gcacgcgcag
     6661 ttcgtccacg gcggcggcgt cggccgcctg ggcaatcagc gcgtccgcgc tttgccggat
     6721 gttttgcaat gcttccttca taccgttctc tcctttgtat gttttgcggg acgaagcgga
     6781 aaaagaaaaa gccccgcccc tgcgctttga cagggacgaa gcttacctgt tcgtaaactc
     6841 cgcggtacca cccacgtttt tgccgtacga ttgtacaaga gcaaacactc tgttgttctg
     6901 taacgggaac acccgtctct gcctaccagc cgcagccttc agcaaagcca ctcgtgagtg
     6961 aacttcggcg cgaaaccatc cccgggcgct tgcagcctgc ggcgcccctc tctgcaggag
     7021 gacagaacct cgcgcgtact ctctcagtca acatgtttga cgcatttatt atacaagcct
     7081 ttgcgccgga ttgcaaggct tttttggcag aaggacgccg gacgcggggg aaatggcgga
     7141 gcggcggaat ggtatatgat aaaagaaaat aaaaggaaag ggcgcggtgc aaaaaggcgt
     7201 ttgccggggc tgcgttttct gtaaagcaac agaactttac atagcttttc cccgggcatc
     7261 tcttttcctt ttttgcattt tgcagcgacg gattttacat tcctccgctt cctttttgca
     7321 cgcggatggg atatactgaa actagagcgc agtgtggaag ggaaacgagg gggagcggcg
     7381 gatgatcatg ggcatcggcg tggacatggt aagcgaggcg cgcgttgcgc gaagcatcaa
     7441 gcggccgggc tttctggaaa aggtatacgg ccctgcggag cgcgctttgc tggagggccg
     7501 gggcatgcgc ccgcagacgg cggcggccaa ttttgccgca aaggaagcct ttggcaaggc
     7561 acttggcaca gggcttttgc gggagttcgc actgtgcgag gccgaggcgc tgcgggatga
     7621 aaacggcgcg ccgtattttt ccttttcagg ccgggcggcg gcgctgatgc aggcgcgcgg
     7681 cctgacggcg catctttcgc tgacccacga gggcggcacc gccgcggcct ttgtggtgct
     7741 ggagcggcgc gaggcatcgg cctccctgaa ggaggaaccg gaatgcagca cattacggta
     7801 gtgccttacg acccagcctg gcctggcctt tatgaagcgg aggcgcaggc catcgcgggg
     7861 gtgctgggac agcggctcac ggccatccat cacatcggca gcacggcggt gcccgggctg
     7921 gctgcaaagc ccgtcatcga cattatgccg gtggtgcggg ccgtcacgga ggccgatgaa
     7981 tgccgcgcgg atttcgaggc gttgggctac gaatatttgg gcgagttcgg cattccgggc
     8041 cgcaggtatc tgcgcaaggg cggcgatgaa cggacgcatc agatgcatat ttttgcctcg
     8101 gacgatgtgc acgccattgc gcggcacttg gccgtgcggg attatctgcg cgcccatccc
     8161 gcccgcgcgg cggcttacgg cgcgctcaag ctggaactgg cggcccggtt cccttatgac
     8221 atcgacggct actgcgacgg caaggacgcc tttgttgggg cgctggagcg cgacgcgctg
     8281 gaatggcgcg ccgcctgtga tttgaaaaat gggagcaaca aagcatgata acaccaaccg
     8341 agatcaaaca aaacgaacag atccgcactt acattgaaaa ggcagacgaa gcgctggccg
     8401 cgctgggcta taccgagcac agctacgcac acgtgacgcg cgttgcgcat tttgccgaaa
     8461 agatcatggc cgacctgggc tatccccggc gcatgcagga gctggcgtgg atcgccgggt
     8521 atatgcacga catcggcaac gtcattaacc gcatcgacca cgcccagagc ggcgcggtga
     8581 tggcgttccg tattctggac aagctgggca tgcccgcgga tgagatcgca accgtgtgct
     8641 cggccatcgg caaccacgac gaaagcacgg ccttccccgt gaacgctgtg gccgccgcgc
     8701 tgattttggc tgacaaaacg gacgtgcgcc gcacccgcgt gcgcaaccgg gacaaggcca
     8761 cctttgacat ccacgaccgt gtgaactacg cggtggaaaa ggccagcacc acgctggacg
     8821 ccgccggaaa ggccgtagtg ctggacctgc agctggacac cggtatgtcg cccgtcatcg
     8881 attattttga aatattcctc gagcgcatgc tgctgtgccg caaggccgct gaaaagctgg
     8941 gcctttcctt ccggctggtg gtgaatggcc agacggtgct gtaagcgcgg cgaaaaacgg
     9001 aacaatgtgc cgtgttttgg cgcatgtgat taagcgcctc ccggcgcggg aaagctattc
     9061 ttgccggcaa gcaggaagtt tttcacgcag ggaggttttt gattggagca caaggaagta
     9121 aagcgcggcg aggtatttta cgccgacctg agccctgtcg ttggcagcga gcaaggcggc
     9181 gtgcgccccg tacttatcgt gcagaatgac gtgggcaacc gccacagtcc cacggtgatc
     9241 gcggcggcta tcacgtccaa gcaggataag acgaacctgc ccacccacat tggcatcaag
     9301 gcgggcaccg ggggcctcac gcgggacagc gtagtgctgc tggagcaggt gcgcacgttg
     9361 gacaagcgcc gtctgcggga gcgggcgggg cagatagacc ccgacgtgca gcgccgtgtg
     9421 gacgaggcgc tggaaatcag cttcgggctg gagacgttct agggcagcgc cgcacaattc
     9481 atggctgacg ccttaagtgt cgcc
//
LOCUS       DAWBSB010000076         9426 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1615_length_9426_cov_3.872479, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000076 DAWBSB010000000
VERSION     DAWBSB010000076.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 9426)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9426)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9426
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1615_length_9426_cov_3.872479"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1933 a         2828 c         3063 g         1602 t
ORIGIN      
        1 aaaaaagcgt agccatgatc catgtggccg atatcaataa ggtcaccggc tatgtccgcg
       61 gcggatgcag cccgctgggg atgaagaaag cctaccgcac cgtgttcgac cgcagcgcgc
      121 ttgcgggcgc aagcatcatc gtaagcggcg gcaggatcgg ctatcagatc gagcttgcgc
      181 ccgaggaact gatccgcctg gcgcgggcgg atacggcgga tatcatctgc ggctgacggc
      241 gccggttccg cattgtaaaa tctgtgtaag acaatcaacc aaaaaagccg cccggcggca
      301 taaggttctt gttgttttct ctgaaaattt gcgccgccgc cggaaagcga ttgcttttat
      361 tccggtttca tagtacgatg ataatatcct ttttcttcgc accgaacaag ctgttcagag
      421 gaggtaccgg gatgcaatca atcgctttgg gaaccgctgc gtacccgggg cggagtgtaa
      481 gccgccggcc ttcccgccgg ggcagtgcgt ctgcgggata ccgcgcgttt tttcggcgcg
      541 cctgcgggct gcggaaagag gatgctgcgc tgaaactgca agcataaggc catggtgtaa
      601 ctgcaccacg gccttttttc ttttcaggga cgcacaaagg agatggattt atggaaaaca
      661 ccaaaaaagt agccatcatc atgggctcgg acagcgactg gccaacggta aaagccgcgg
      721 ccgcacagct caaaagctgg gatatcccgt ttgaggtgcg cgtaatgagc gcccaccgca
      781 ccccggccgc tgcggcggag tttgcaaagc gcgcggcgga aaacggcttc ggcgtgatca
      841 tcgcggccgc gggtatggcc gcccatctgg cgggcagcat cgccgcgaac accgtgctgc
      901 ccgtcgttgg catccccgtt aagggcggcg cggtggacgg cttggacgcc ctgctggcca
      961 ccgtgcagat gcccagcggc attcctgtgg ccaccgttgc gctgaacggc gccaaaaacg
     1021 ccgccgtgct ggccgctcag atgctggcca tccacgacgc gcgcatcgcc gcgctgctgg
     1081 cggaggaccg ggagaaaatg gcccgctcca tcgctgaaaa ggacgccgcc atccgggccg
     1141 aggcggccgc gctgtaagga atgctttcca aatatctttt tatttttata aaacacaaaa
     1201 gagggagaag aacaacatgg aaaaacggga acagctgtat gaggggaaag ccaaaaaggt
     1261 attcgccaca gacgacgcgg accttgtcat cgtggattac aaggacgacg ccaccgccgg
     1321 cgacggagca aagaaaggca ccattcgcgg caagggcgtg gtgaacaaca ggctttccaa
     1381 caacctgatg aagatgctgg ctgaaaaggg cgtgcccacc cactttgtgg aggaattgtc
     1441 cgaccgcgag acgctggtga aaaaggtgga gatcgtgccg ctggaggtca tcatccgcaa
     1501 cgtggccgcg ggcagcttca ccaagcgtct gggcgtgcct gagggaacgg tgctgaagat
     1561 ccccaccatc gagttcagct acaaaaacga cgagtatcac gaccctctca tcaaccatta
     1621 ccacgcgctg gccctgggcc tggccacgca ggaggagatc gacaccatca cgaaatatgc
     1681 cttccaggtg aacgacatcc tttcccaata tctgctgggc ttcaacatcc gcctcatcga
     1741 cttcaagctg gaattcggcc gcctgcccga cggctccatc gtgctggccg acgagatcag
     1801 ccccgacacc tgccgcttct gggacgccac cacgggcgcg cacctggata aagacctgtt
     1861 ccggcgcgac ctgggcggcg aagaggaagc ctaccgggaa gttatgaagc gcctgctggg
     1921 ggagtgaacg gatgatcaaa gactatcttg acgaaaagct gcatgaagag tgcggcgtgt
     1981 tcggcgttta ccgccgcgac gcggacctgg acgtggtggg taccacctat tacggcctgt
     2041 acgcgctgca gcaccgcggc caggagagct gcggcatggc ggtgaacgac gacggcgtgt
     2101 tctacaaacg gcgcgagctg ggccttgtga acgacgtgtt caccaaggac gtgctggaga
     2161 cgatgccccg gggcaatatg tgcatcggcc actgccgcta tgccaccacg ggggtgcccg
     2221 tccgctccaa tgcgcagccc tttgtgatcc gccacatcaa gggaccgatg gcgctggctc
     2281 acaacggcaa cctgacaaat tccgccgagc tgcgttcgga attcgagctt tcgggcgcga
     2341 tcttccactc cacgtcggat acggaggtca tcgcttacgc catcacccgc gagcgccttt
     2401 cctccgacag catcgaacag gccgtggagc aaacgatgga caggctgaag ggggcgtatt
     2461 ctctggtggt gatgagcccc cgcaagctca tcgccgcgcg ggacccgcag ggcttccgcc
     2521 cgctgtgcat cggcaagctg ggcgaaaacg acatcattgc cagcgagagc tgcgccatca
     2581 ccgccgcggg cggcgagttc gtgcgcgatg tggagccggg cgagatcgtg gtgtttgacg
     2641 aaaacggctg ccgctccatc cgcacccact gcggctgccc cacaggcctg tgcgtgtttg
     2701 aatacgtgta catcgcccgg ccggattctg ttgtggacgg cgcttccgta caccgggcgc
     2761 gccggcgagc cggcgcgttt ctggcgctgg agcaccctgt gcaggcggac gtcgtcatcg
     2821 gcgtgccgga ttcgggcctg gacgcggcgc tgggctattc ccagcagagc ggcatccctt
     2881 acggcatcgg ctttttgaag aacaaataca tcggccgaac gttcatccag cccaaccaga
     2941 agctgcggga gaataccgtg cgcatcaagc tgaaccccat tgcggacacc gtgaagggcc
     3001 ggcgcgtggt gctggtggat gattccatcg tgcgcggcac cacctcgcgg cagatcgtac
     3061 agatgctgcg ggaagccggg gccacggaag tgcacttttt gtcctccgcg ccggaatttc
     3121 tgtatccgtg ctatttcggc gtggacgtgg acagccggga aaatctcatc gccgccaacc
     3181 attcgctgga ggagatgcgg gagattttgg gtgtggacag cctgggcttt ttgagcgtgg
     3241 acggagtgaa gaagatcgcc gagggctgta aatgcggcct gtgcacgggc tgctttacgg
     3301 gcaaataccc ggtggagccg cccaaaagca ctgaaaaaag caaatttgaa aagaaaattt
     3361 ccgaaaatat caacgactga ccgtcgggaa caggagaaac gatatgaaga gcttttccga
     3421 gagctacaag gcagcgggcg ttgacatcac ggcgggctat gccgcggtgg agctgatgaa
     3481 acagcatgtg gcgcgtacga tgacgccggg cgccatcggc gggctgggcg gcttcggcgg
     3541 cctgttcgag ctggatatga cgggcctcac ccacccggtg ctggtctccg gcacggacgg
     3601 cgtgggcacg aagctgaaga tcgcttttct gatggacaag cacgacaccg tgggcatcga
     3661 ctgtgtggcc atgtgcgtca atgatgtgat cacctgcggc gcgaagccac tggtatttct
     3721 ggactatatc gcctgcggaa agaattaccc ggagaagatc gcctccatcg tggcgggcgt
     3781 ggccgagggc tgcgtccagg cgggagcagc gcttgtgggc ggcgagaccg ccgagatgcc
     3841 gggcttttac ccggagcagg aatacgacct ggccggcttt accgtgggcg tcgtggaaaa
     3901 aagcaaaatc attgacaata cgaagatgca ggcgggcgac gccgtcatcg cgctgccctc
     3961 cagcggcgtg cactccaacg gcttttctct tgtgcgcaag atattcaatg tggagtacgc
     4021 ggatatctgg cagcattacg acgagctttc cggcacgctg ggcgaaacgc tgctcacgcc
     4081 cacaaaaatt tacgtcaagc ccgtgctggc gctgatggag caggtggatg tgcgcgccgt
     4141 gagccacatc acgggcggcg gcttttatga gaacatcccc cgcgcgctgg ccgcgggctg
     4201 cgtggcgaag ctcgagaaaa agagcctgcg catgccgccc atcttcccgc tgctgcaaaa
     4261 ggttggcaaa attcccgagc gggacatgtt caatacgttc aatatgggcg tgggcatggc
     4321 tgtcgtcgtg cccggcgaca gggcggacga atccgtgcgc atcctgaagg aagcgggcga
     4381 ggatgcgtac atcctgggcg agatcgcgaa gggtgcggaa aagggcgtag agctatgctg
     4441 aacgtagcgg tgctggtttc cggcggcggc acgaacctgc aggccattct ggacgcaaag
     4501 gccgcgggcg cgctgcccca tgcgaaaatc gctctggtgc tggcctcaaa gcccggcgtg
     4561 tacgcgctgg agcgcgcgtc caaagcgggc gtgcccggca ttgtggtggc gcgcaaaagc
     4621 tatgccgcac cggaggaata cgacgcggcg ctgctggccg cgctgcggga gcaccgcatc
     4681 gacgtggtgg tgctggcggg ctttttgtcc atcctgggcc ccagcgtcat caccgcgtat
     4741 cccgagcgca ttctgaacgt gcaccccagc ctgatcccca gcttctgcgg ggcgggctat
     4801 tacggcctgc gggtgcatga ggcagcgctg gcaaagggcg tgaaggtgac gggcgcgacg
     4861 gtgcattttg tgaacgaagt gccggacgga ggacgcatcc tgctgcagca ggccgtggac
     4921 gtgctgcccg gcgatacgcc ggaaacgctg cagaagcgcg tgatggagca ggcggaatgg
     4981 aagctgctgc cccgggcgct ggcgcagctg acggaagaat tggacgcggc ggacggaccc
     5041 gccgcgccgc ggaaagagga gaaggatatg gaccatctga gccttgcggc ggagcttgcc
     5101 gtaaatacat accccggccg cggcatcgtg ctgggaaggt cggaggacgg caaaagcgcc
     5161 gtcatcgcct attttatcat gggccgcagc gcaaacagcc gcaaccgcgt gttcaccgcg
     5221 aaggacggcg gcatcattac cgaagcggcg gacccctcca agctggagga cccgtccctc
     5281 atcatctacg cgcccgtgcg cgtgctgggc aaaacaacca tcgtgaccaa cggcgaccag
     5341 acggatacga tatacgacca cctggccgcg ggcaagggct ttgccaaggc gctgcgcacg
     5401 cgcacctttg agccggattc ccccaacttc acgccgcgca tctcgggcat tgtgaaggtg
     5461 aaggacggcg caatgaagta caagctgtcc attctgaaat ccgacggcgg caacgcggat
     5521 tccgtggaac ggttcttctt tgaatacgac cagcccgttg caggcgaggg ccgctttatc
     5581 catacctacc gctgcgacgg cagccccatc cccagcttcg cgggcgaacc ggagcgcgtg
     5641 cgcctgatgg gcgatatcga cacgtttaca agaatggtct ggaacagcct gaacgaggac
     5701 aacaaggttt cgctgtttgt gcggtatatc gaccttgcca cgggcaaaac gcaggaccgc
     5761 atcgtgaaca aatacgaaaa agtgtaacgc gccgccccgt gtgcggcagg gccgccgggg
     5821 acatcgaatg ggggttttta aggatatgaa agaactggaa ctgaaatacg gctgcaaccc
     5881 gaaccagcgg cccgcgcgca tcttcatgcg ggagggcgag ctgcccatca ccgttttgaa
     5941 cggcaaaccg ggctatatca atttcctgga cgcgctgaac gcgtggcagc ttgtaaaaga
     6001 gctgcgccag gccacgggcc tgcccgcggc cgcgtcgttc aagcacgtca gccccgccgg
     6061 cgcggcgctg ggcctgccgc tggacgatac gctgaaaaag atgtattttg tggaggacat
     6121 cgagctttcc ccgctggcct gcgcctatgc ccgcgcccgc ggcgcggacc gcatgtccag
     6181 cttcggggac tggatcgccc tttccgacga atgcgacgaa gccacggcga aggtcatcca
     6241 gcacgaggtg tcggacggcg ttatcgcgcc gggctattcc gccgccgcgc tggccatcct
     6301 gaaaacaaag cgcaagggcg gttacaacat tgtcgccatc gacccatcct accagcccgc
     6361 acctgtggag cacaaggatg tgttcggcat cacgtttgag caggggcgca acgagctgaa
     6421 gttcggcgac gatacgttct ccaatatcgt cacacggaac agggcgctga cagacgagca
     6481 gaagcgggac ctgacgctgt gcctgatcgc gctgaaatac acccagtcca actcggtgtg
     6541 ctatgcccag ggcgggcagc tcatcggcgt gggcgcgggc cagcagagcc gcatccactg
     6601 tacgcgcctt gcgggcaaca aagcggacat ctggcagctg cgccaccacc ccaaggtact
     6661 gggcctgccg ttccgtcccg acgtctcccg ccccaaccgc gacaacgcca tcgacgtgta
     6721 tatctccgac gaatgggagg acgtgctggg cgacggcgtg tgggcggaga ccttcaccac
     6781 acgtcccgaa ccgttgacgc ccgccgaaaa aaaagaatgg ctgtccaagg tcacgggcgt
     6841 ctgtctcggc agcgacgcat ttttcccctt tggggacaac atcgagcgcg cgcgccgttc
     6901 cggcgtgacg gccatcgtgg agccgggcgg ctccatccgg gacgggcagg tcattgaaac
     6961 ctgcgataaa tacggcattg tgatggcgtt caacggcatc cgcctgttcc accattaaaa
     7021 agaacgccgc gcggccgcgc cggggcggag cacggctctg cccgcgtttg gcggcgcggc
     7081 ggcgaaagca gggaggttga cctatgaagg ttctggttgt cggcgggggc gggcgggagc
     7141 acgctatcat ccgcaagctg aaggaaagcc cgcggcgccc cgcgctgtat gccgcgccgg
     7201 gcaacggcgg tatcgccagc gacgccgtgt gcgtgcccat cgccgccacc gacgtggcgg
     7261 gcgtggtgtc ctttgctgtg caggagggca tcgattatgt ggtcgtcgcg ccggacgacc
     7321 cgctggtgct gggcatggtg gacgcgctgg cggaaaaggg cattcccgcc tttggcccca
     7381 acaaggccgc cgccattctg gaaggcagca aggtgttcag caaaaacctg atgaagaagt
     7441 acggcatccc cacagccgcc tacgagacgt ttgcggacgc gggcgaggcc gcggcttatg
     7501 tgcgcgccaa ccggaagtac ccgcttgtcg tcaaggcgga cggccttgcg ctgggcaagg
     7561 gcgtgctgat ctgcgaggac gaggccgcgg cgctggccgc tgtgaaaagc atcatggagg
     7621 acaggcagtt cggcgcgtcg ggcagccgta tcgtcatcga ggagtttctc acggggccgg
     7681 aggtttctgt actcagcttt accgacggaa aaaccgtcgt gcccatggtg tccagcatgg
     7741 accacaagcg cgcgctggac ggcgacaggg gcctgaacac cggcggcatg ggcaccatcg
     7801 cgcccaaccc ctattatacg ccgcagatcg cacaggtgtg catggagacg attttcaaac
     7861 ccaccgttgc ggccatgaac gcggagggcc ggcccttcaa aggctgtttg tatttcgggc
     7921 tgatgcttac gcccgacggc ccgaaggtca ttgagtacaa ctgccgtttc ggagacccgg
     7981 agacccaggt ggtgctgccg cttctggaga gcgacctgct ggctgtgatg caggccgcca
     8041 ccaacggtac gctggcgcag acggaagtaa aattcagcgg ccaaagcgcc gcgtgcgtgg
     8101 tgctggcttc aggcggatac ccgcagcact atgaaaaagg aaagcccatc acgggcctcg
     8161 cggacggcca gctgccgggc ggcggggcca ccgtgtacca cgcgggcacg gcccttgcgg
     8221 acaatgtgct cgtcaccagc ggcgggcgcg tgctgggcgt caccgccgtg gcggattcgc
     8281 tgtttgacgc cgtggccgcg gcctacggcg cggcggacca gatcggcttt gaggggctgc
     8341 accgccgctc ggacatcggc cagaaggcgc ttgccgcgct gtgagccaca gggaggaaac
     8401 gttatggtat atcgcattta tgtggaaaag cgtcccgaat acgcagtgga ggccaggggc
     8461 attttgtccg agatccgtca tctgctgcgc atcaaaagcg tgacgggcct gcgcctgctg
     8521 aaccgctacg acgtggaggg catctccgaa gagctgtttt cccagtgctg cggcaccgtg
     8581 ttcagtgagc cgcagctgga cttcacctat acggccgtgc ccgaaggcgc ggacgcggtg
     8641 ttcgccaccg agttcctgcc cggccagttc gatcagcgcg cggccagcgc cgccgagtgc
     8701 atccagctca tcagccaggg cgagcggccc gacgtgcagt ccgcccgcgt gttcctgctg
     8761 tacggcgcgc ccagcgcgga ggagctggct gaaattaaaa aatacgtcat caaccccgta
     8821 gaggctcggg aggcgtccct tgcggagaag gccacactga agaccgatta cgccatccca
     8881 accaccgtgg aaacactgga gggcttcacc gcgctggacg cggccggcct tgcggatttt
     8941 gtgaagcgct acggcctagc catggacgag ggcgacatcg ccttctgcca gagctatttc
     9001 aaaagcgagc atcgggaccc cacgatcacc gagatccgta tgatcgacac ctattggagc
     9061 gaccattgcc gccacaccac cttcggcacc atcctagacg acgtgcagat cgaggacgag
     9121 acggtgaagg ccgcatggga gcgttatctt gcgctgcgca gggaggtgca cgcgggcaaa
     9181 aacaagcccg tatgcctgat ggacctgggc accatcggcg gaaagtacct gaaaaagacc
     9241 ggcgcgctga agaacatgga cgaatccgac gagatcaacg cctgcagcgt gcgcatcacc
     9301 gtggacaaca acggtacgga cgagccctgg ctgctgctgt tcaaaaacga gacgcacaac
     9361 catcccaccg agatcgagcc cttcggcggc gcggccacct gcattggcgg cgccatccgg
     9421 gacccg
//
LOCUS       DAWBSB010000077         9370 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1631_length_9370_cov_3.318948, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000077 DAWBSB010000000
VERSION     DAWBSB010000077.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 9370)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9370)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9370
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1631_length_9370_cov_3.318948"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1370..1379
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3480..3489
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4649..4658
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8768..8777
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1821 a         2684 c         2943 g         1882 t
ORIGIN      
        1 accgcaggcc gcgggctttt cttcgggctt gtccccggcg ccgcaggctg aaccgcaggc
       61 cgaggcggca gcctccccat ttttttgtgc ccagccaaac agattggata gtgccatgat
      121 cgttacctcc gtctttttta tttgagggat gttgtttgtc cctctgactg tattgtaaaa
      181 gccggcggca aagaaaaaaa gtacgcactt ttttattaca tagttccctt ttggtgacct
      241 tcgcttgcgg tgaaagcacc tttgtattat aatttgaatg caatattcat atttaaagca
      301 ttggaaagga gcactccctg atgttgacaa aggaagaaat gccggcctgt ccggtcgcga
      361 ccaccgtgca gctgatcggc agcaaatgga agctgctgat catgcggaat ctgctgcagc
      421 ggccgtggcg tttcaatgaa ctgcggaaaa atttggaggg catcagccag aaagtgctca
      481 ccgacagcct tcgctctatg gaagaggacg gcatcattac ccgaacggtt tatcccgaag
      541 ttccgccccg cgtggagtat gccttgagtg cgttgggaga atccatgcgg cctatcatgg
      601 acgcgatgga agtgtgggga aacaactaca aaaaccaggg atgagaaaaa gcccggatac
      661 ccgcctttgc aggtgggaca tccgggcttt tccttttcta taaaactgcc gcgaggggaa
      721 aggctgtgcg ccgaaaggag ttgacaaatc aaactgtaac tgatagtata acaattaaac
      781 tgagataaca aaagttacag ttgtccggcg gcaatggccg tgcggagcac ttatagcgga
      841 attaaaaagg agcggaaaga tgacaagcga attcggcatc gcggtacacg cgatggtatt
      901 tctgcatcac aagggggata tggtatccag cgaggtgctg gcggagaata tttgtacaaa
      961 cccggcccgg gtgcgcaagg tgatggccca gctcaagcgg gcggggctgg tggaaacgca
     1021 cgagggcgct gtggggggtt accgctttgc gggctcggcg gataccgtga ccctgtgcga
     1081 cattgcgcag gcggtaggtg ctgcgttcgt ttcggcatcg tggcgcagcg gcgacgtgga
     1141 taagccctgt cttgtggcaa gcggcatggg cgcattgatg gacgaagtgt acacaggcct
     1201 ggacgcgctg tgcaaggaac gtctgcaaag cgtcacggta gccgcgctgg aggcgcggct
     1261 gttcgacggc aagccgctgg ccgggccgtg cggccgggca aaaaaagaag aagctgccgc
     1321 ggcgcacggc ggggcgaagt aaaaggctcc cgcaggcggt tttcattccn nnnnnnnnng
     1381 gaaaggattt ttatataaaa caataaaaaa tggccggagg ctatttttta ggccatgcgg
     1441 gccgtccccg gcccgcggcc atcaagatga acctttttat tgcggaattt ttttaaatat
     1501 aacaaaaagg agacttctat gggttttact cttgaaacag gcgtttccgc cgcagcggta
     1561 tttttgcagg gcatcctcag cttcttttcc ccgtgcgtgc tgcctctggt accactgtac
     1621 atcgggtatc tggcgggcgg cgcgaaaagt gtggacgccg acggcacagt gcgctacaaa
     1681 cgcggccgtg taatgcgcaa tacggtgttt tttgtgctgg gcgtcagctt tacctttttt
     1741 gcgctggggc tggggtttac ggcgctgggc cgctttttct ccgataaccg cgcgctgttt
     1801 gcgcgcatcg gcggcgtgct gatcattctg ttcggcctgt accagctggg cgtgttccgt
     1861 tcccgcgcgc tcagcaccga acgtcgcctg cccatccgtc ttgaccgtct ctcaatgaat
     1921 cccgcggtgg cgtttctttt cggcttcacg ttcagttttg cgtggacgcc gtgcgtcggc
     1981 ccggcgctgg gcagcgtgct catcatggca acgtcggctg gctcttccgc acagggcttt
     2041 ttgctcatcg gcgtatatac gctggggttc gtgctgccgt tcctggcggt agggcttttc
     2101 acgggctcgc tgctggattt tttcaaaaaa caccagaaag cggtgcgtta taccgtcaag
     2161 gcgggcggtg tgctgatgat cctgatgggc gtaatgatgc tgacaggctg gatgaacggc
     2221 tttaccagct atctctccgg cttgggcggc gctgggacgt cggcgtcttc cgccgcccct
     2281 tcgtcttccg tgccgtcaga cagcgcggcg ggacagggag gcggcgcatc gtccgggccg
     2341 gataatgcgt cggcgtcttc cggctctgca gccagctcgg agcagccgga gatccccgca
     2401 ccggatttca cgctgacgga ccagttcggc aagacgcacc cgctttcgga ttatcagggc
     2461 caggtcgtat tcctgaactt ctgggcgacc tggtgcggcc cctgccagcg ggagatgccg
     2521 gacatccagg cgatgtatga ggatctgggg ctgaacgagg aagacgtggt cgttctggcc
     2581 gtggcgaacc caatatccgt ataatcagga tgtaagcgaa gacgaggtga agcggtttct
     2641 ggagggaaac ggctatacct atcccgtggc gatggatacc acgggcgagg tgttcgcggc
     2701 atacggtgtg cgggcctttc ccacaacgtt tatgatcgac cgggagggca atgtgtttgg
     2761 ttacgtggaa agcacattga cgcgggacat catggacagc atcgttcagc agacgctgga
     2821 cggcagacgc gcgggctgac ggaaccggcg gaaaataagc aggcgccccg gcgggaaaat
     2881 gcccggggcg ccttgcttga tttcatctga ttggtgggcc gggaacggat gtcccgcagg
     2941 aaaggcttac agagaggcca tctgtgccgc aatatcatcc tcgcaggccc gcatggcctg
     3001 cagcgtgctg tccagaagcc tgtccagctc ccagccaagg cgttccgcgc cctggcggat
     3061 gacgtcgcgg gagcagcccg cggcgaattt tttgtctttg aattttttct tcaggctgga
     3121 aagctccata tcctgcacgc tcttcgaggg gcgcatcagc gcggccgcgc cgacaaggcc
     3181 cgtcagctcg tccgcggcaa ataacacttt ttccatttcc agcacgggct ccacatcgct
     3241 gcacaggccg tagccgtggc tgcaaaccgc gtgtacgaac gcatcgtccg cgccaatctc
     3301 agccaacagc tcgggcgctt ttttgcagtg ctgttccggc cactgctcaa aatccacgtc
     3361 gtgcagcagg ccgaccagcg cccagaaatc cgcttcgccg ccgtgcccca gctcgttcgc
     3421 ataccagcgc atgacgccct ccaccgtgaa agcatgctgc aagtggaacg gttctttgtn
     3481 nnnnnnnnna aattggcttt attgtagcag atactgcgca aaaacaaaag aggggagctg
     3541 aacaaaacgc gccgcagaga ggacggcctt gcgtcctaca ccgcagcgcg caggggagag
     3601 atcacggcat tttgtgctcg cagtacacac agcgcggcac accgtgagcg tcggggacgt
     3661 acaggggcac aagctcttct tcggcaaccg tgatacagcg ggggttccgg cacggcgcac
     3721 ccgttgcctc ggcggcgcgc tgtgcggaaa gagcctgctc ggccagcggg ccggagggcg
     3781 ccaggcccag cagcgtcagg atcagtgcca tacgcacata cacgccgttc tgcgcctgct
     3841 caaaataggc ggcgcgaggg tcgtcgtcaa ccgccagcgc gatctcattt acgcggggca
     3901 gcgggtgcag caccgccatg tctgctttgc ccagcaccat tttgggcata tccagcacat
     3961 aactgccttt caggcgttca tattcgccgg cgtcggtaaa acgctccttt tggatgcgcg
     4021 tcatatacag gatgtccaag tccgccatgg cttcctccag tcccgtcacc tcggcatagg
     4081 ggatgtgcgc gggcttgaca acgttttcga taaggtggcc gggcacgcgc agctcctcgg
     4141 gactgatgaa cacgaattca ttgccctcgt aacgggccag cgcattcacc agcgagtgca
     4201 cggtgcgccc gtatttcaaa tcgccgcaga agccgatctt caggtgggac aggcggcccc
     4261 ggcgcgtcag gatggtgagc aggtccgtca gtgtctgggt gggatgctgg tggctgccgt
     4321 cgccggcgtt gatgacgggg atgcgcgaaa agcgtgccgc gcgcatgggc gcgccctcca
     4381 agggatggcg catggcggca atgtccgcgt agcaggagac gatacgaatg gtatcggcca
     4441 cgctttcgcc cttggaaacg ctggatacgt tggcactggg gaagcccagc acgctgccgc
     4501 ccagattgag catcgcggct tcaaacgaaa gacgggtgcg ggtgcttggc tcgtaaaaca
     4561 gagtggcaag ctttttgcct ttgcagcttt ccgagtaggc tcccggctca cggcggatat
     4621 cttcgcccag ccgcagaagc ctgtccagnn nnnnnnnnct cttccacggt aaagtccagc
     4681 gggtcgatca aatggcgcat atacattccc tccaagaatc cctcgcgggc acggcagccg
     4741 cgcccggctt gctgcaggcc gcgcggtgac acgccatgct tcccggcctg cacctcgcat
     4801 tcaccgggca gacatgtttg ccgtacagcg gcaatttctt ggacattata ccgcgccggg
     4861 cggcggttgt aaagggcgtt tcttatttta aaacacgccg cagcatgcgc tccttttcct
     4921 ttgtataggg ctggtaacgc acgggaagat cgagccggtt tgattttttg acaatgcttt
     4981 tgtaatgtgt gaacgtatcg aagctgagct tgccgtggta actgcccatg ccgctttcac
     5041 ccacgcctcc aaagcccatg tcgctggtgg caaggtggat gatggtgtcg ttgatgcatc
     5101 cgccgccgaa gctgcagtcc cgcagcacgc ggcgctctgc ggctttgtcg gtggtaaaca
     5161 ggtacagtgc caggggcttc ggccgcgcgt tcacgaacgc cacggcctca tccaggcttt
     5221 cataggccag cacgggaagg acgggcccga aaatttcctc gctcatcacc gggctgtcgg
     5281 gtgaaacatc cgccagcacc gtgggcgcga tctgcagcgt ttcggcgctg cccgtgccgc
     5341 cgcataccac gcgtccgctg tccatcaggc ccatcaggcg gtggaaatgc ttttcgttca
     5401 cgatgcgtcc gtaatccggg caggccagcg ggtcgctgcc gtaaaaggcg gcaatctccc
     5461 cggccagaag cgtcagcagc ccgtcatgaa cgtccctgtg cacgagcaca taatccggcg
     5521 cgacgcaggt ctgcccggcg ttcaggtatt tgccgaaggc gaggcgcttt gccgccaggg
     5581 ggaggtctgc ggtttggtca atgatgcacg ggcttttgcc gcccagctcc agcgtcacgg
     5641 gcgtgagcca gcgcgcggcc ttttccatca ccagcctgcc aacggccaca ctgcccgtga
     5701 aaaagatata gtcgaatttc tgttccagca gggcttcgtt ttccgcgcgt ccgccctcca
     5761 cgacggcgat atggtccggc gcgaagcagc tttccacaag ctccttcaaa acgcggctgg
     5821 tggccggcgc gtacgcgctg ggcttgagca ccgcgcagtt gcccgccgcg atcgcgccga
     5881 ccaacgggtc cagcgagagc atgaacggat aattccacgg gctcatcacc agcgccacgc
     5941 ccagcggctc gggcacgacg aagctgcggg cggggaactg cgccagcggg gttttggcgc
     6001 gcctggcacg catcaggcgg ttcagatgct tttccatata ggtcagctcg ctcagtacaa
     6061 ggcctgtctc gcacatatag ctctcaaagg gagatttgtg caggtcctcc ttcagggcgg
     6121 cgcagatatc ggcctcccgg gccgttaccg cctgcctcag cgcggccagc gcgcggcggc
     6181 ggaaagcggc gtcttttgtc gcgcctgtat tgaaaaaagc cctctgggcg gataccagct
     6241 cttcaatctg catgccttgg ccctcctgtg gtttatagtg gtatgggttg ttgctgtcag
     6301 tatagcatgt tataatgcac tcgtaaacat tgtgtttgca ggagcggcgc cgcggctttc
     6361 cgaaaacgaa cgcgcactgc cgtgcggccg gcgcctgcgg aatacttgta aaacagagga
     6421 aaggcgggaa tgatatgttt acattgcccg aatactgcgc gccggatttt tccgcgccgc
     6481 ttttacaaaa tgcgcccagc gtgcgcacgg caccggcccc tgcggacggc gccgcgcctg
     6541 cgggctatca tgccacaggc atgtatccgg aatattataa agtggacggc gtgtggcgtc
     6601 ttgctgaaaa aagccgtatg gactgcgtgc ctgttctggc cccggacggg gaactgcgca
     6661 ttgtggagtt ccgcaatctt gtgcagggcg acagcgtggt gctgggccgc acggacgatg
     6721 gcagcgaggg cattctgatg catacggagg gcttttccgc gggcggcaaa agcgccgaag
     6781 cgttcgcgtt ccgcaccggg cgcagccggg aaacggcttt cacgcgggat tatgacgcac
     6841 tggccgggct gctgcgccat gaaaaagagc acggcaatgt ggtatgggtg ctcggtccgg
     6901 cgtgcagctt cgacgcgtcg gccaaggcgg ccatgcaggc gctcatcgaa aatggctatg
     6961 cccacggcct gctggcgggc aacgctcttg ccacgcacga cctggagggt tcgctgctgc
     7021 acacggcgct ggggcaggat atctacacgc aggagagccg ccccaacggc cattaccacc
     7081 acatcgacgt catcaacgcc gcgcgggctg caggctccat tccggcgttc atcgaacaga
     7141 acggcatccg ggacggcatt atgcatgcct gcgtggcgca cggcgtgccc tatgtgctgg
     7201 ccggttccat ccgcgacgac ggcccgctgc ccggcgtata cggcgacgtg tacgcggcgc
     7261 agaacgccat gcgggagctg gtgcagcagg ccaccaccgt catctgtgtg gccacccagc
     7321 tgcataccat tgccacgggc aatatgacgc cttcctactg tgtgcgcggc ggccgggtac
     7381 ggccggtata cctgtactgt gtggatgtct ccgagttcgt caccaacaaa ctgcgggaca
     7441 ggggcagcct ttccagcgtg cccatcgtgg ccaatgcaca ggactttttg tgccgcgtgg
     7501 ccgcagggct cggcttgccg gttgcggaaa aatatgataa aataaaccgt taagggtggt
     7561 gttgctccaa tgagtcagaa atttacgccg cttgcgcagt cccgcgcgag ctattattgt
     7621 aaaggcagcc cggtgcattt tgttatggtg gagctgttcc gcatggaagg cacgggcgcc
     7681 acggtcgtga cgcttacgtt caaaaacctg ttctcccgcc cgctggtctc gttcacagcg
     7741 tatttccgct gcaagaacaa atacggcgaa gtggtggtgg aggactcctt tacctatgat
     7801 aacgtgtatg ccggggaagg cgagtgcttc ggcttcgacg atgcggtgtt tgtcagcgac
     7861 gagcctttgg gcagcgtgga ggtacgcctg ggctccgtca cctacaacga cggagcgcct
     7921 catgacctgc gccgctgccc ggctgtggcg ctgcctgcgc tgcggcccct gccccagcgc
     7981 gaacgcgcgg ccgtgtgccg cgcgttgggc acgcaggatg cggactatta ccccgaggag
     8041 gctgccgacg gctggcgctg cacctgcggc gcgttcaatt acaacgcggg ccgcggcatc
     8101 cgttattgca gcgagtgcgg cgtggaaaag gtgctgatgc aggcggctgt gcgcgacgcg
     8161 cagagcggcg cggcgccggc acgcccccag tttggtgcgg atatgcagtt tttgcccgtg
     8221 caggacgaag cgcccgccgg cggctccggc gggcagcggg gcggcggcgt gcagtatgct
     8281 gtgcaaaagc cccggatgcc gcgcgcgctg cgcggcgtac cggagcagga aatggaacgg
     8341 cccgccgttc cggccatggg cggcgcaccg caggcctgcg tttccatcat gaaggattcc
     8401 acggcggatt ttatcctgcg gtatatgccc tttgtcacca tggccgcggc cgcggtgttc
     8461 acgatggggg cggtgctggt gtcgcagtat ctgctgtgat gcgggaacgc ccaaaacaaa
     8521 agcagcgttg cgccgggcgg cccagacggg ccgccctgtg cgcggttaca gcatatggct
     8581 atagggctgt tttatagctc tgtacggcaa agcgatcaca aatatgaagc tccaaggagg
     8641 cacaaaccga atggcaagaa aacctgccgc ctataccaat gaaagcatca catccctgaa
     8701 gggcgccgac cgggtgcgca agcgtcccgg cgttattttt ggctccgacg gcgtggacgg
     8761 ctgcgccnnn nnnnnnntcg atgaggcgcg tgagggctac ggcaaccgca ttctcatcac
     8821 caagtttaag gacggctcgg tagaggtgga ggacttcggc cgcggcatgc ccgtggatta
     8881 caataaaaac gagcagcgct acaactggga gctgctgttc tgcgagatgt acgcgggcgg
     8941 caaatactcc gccggcgcgg acaattacga atactccctg ggcctgaacg gtctgggcct
     9001 gtgcgccacg cagtatgcgt ctgaatggat gacggcggat atttaccggg acggtttcca
     9061 ttatcacctg gatttccgca agggggaaaa cacgggaggg ctgaaaaaag agcctgccac
     9121 ccgcaaaaaa acgggctccg ccatccgttg gaagccggat gccgaagtgt tcaccgatat
     9181 caacgtgccc agtgaatatt ataaggacgt catcaagcgc caggccgttg tcaacgcggg
     9241 cgtcaccttt gtgttcaccg acgagagcgg cgagaaaaag gaagtaacgg aatactgcta
     9301 cgaaaacggc atcaaggact atgtggagga agtggccgac cttcaaagcc tgacccccgt
     9361 ggtgttctgc
//
LOCUS       DAWBSB010000078         9125 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1666_length_9125_cov_3.897905, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000078 DAWBSB010000000
VERSION     DAWBSB010000078.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 9125)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9125)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9125
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1666_length_9125_cov_3.897905"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1671 a         2792 c         2875 g         1787 t
ORIGIN      
        1 cccacgatca tggcgtccac catgctgtaa aactgctgga agatattgcc caccagcatg
       61 ggcagagaaa accggagcag cagccgcatg gggctgcccg aagtcatatc cgtaaccaaa
      121 aaagcacctt tcctttcccg aaaagcggcg cttcggagcc gtgtgccccg cgccgcgcgg
      181 agccgaaaac agccctgcac aaaacgctat tctagcccat ccgggcaccg atgaaaagcc
      241 tttttttgaa atttgcgata tttttccgaa ttggacaaaa aagcacggcc cggaaggctc
      301 cgggccgtgc tttttgcctg tctgcagagc ttattcgctt agaatgcccg ccttgcgcag
      361 cgcgggccgc acggcttttg caagcagcac ggacaccacc acggccagca ccgcgttgat
      421 gagggacgtg acgccctttg tcacgagctg cgtctgcacc gcgcccagag cctgcccctc
      481 gatgtaaaat ttcatgatga aggacttcag caggtacagc gccacatacg tcacgctgcc
      541 cacggacgcg cccagcacgt cccgtcccag cgagatatta ccgctccggt gggcgatgag
      601 cccggccaga aagccaatga aaaattttgt gatgaatgtg atccagcttt ccgcgatgta
      661 cagcggattc gtaaagtcga acagcatact gccgaagccg gaggcaaggc cgcccgtcag
      721 gggcccgaac agcaggccgg acagcacgca gaacacattg cccagatgaa tgcgcgctac
      781 ctccccgatg gggatctgta tccagctgaa tacgaatacc agagcgctca gcaggcccgt
      841 gacggccagc ctgcgcacgg tgaaccggtt ggatgttttt tccatgaata ccactcctct
      901 ttgtttttgc cctgcgccgc gaatttccgg ttgacagggc cgccgctttc gcttatcctc
      961 aattatagaa gattgtgaac tgattaaaat actcaattta tgaatatttt ataaggtcag
     1021 attggaggac aggcatggaa agctttacgc tgggcctgat gcgcggggac ggcgcgccgc
     1081 tgtaccgtca gctataccgc catatcgtgg aggagatcgc ttccggcggg ctggcggcgg
     1141 gggaaaagct gcccagcaag cgccgcctgg cagcggacct gcgggtgagc gtttccaccg
     1201 tggaaacggc ctaccaaatg ctggtggcgg agggctacat agcggcccgc gcgaaaagcg
     1261 gcttcacggt atgccgcatt gaaaagctgg acgcacccgt ccgtccggct ccggccgcgc
     1321 cgccggaacc gcccgcaaag cgctgggcct atgatttcgg cacgggcagt gtggacactg
     1381 ccctgtttcc gttcaagaca tgggcgcgca tccagcgcga gacagtcacc gcccatccgg
     1441 agcttctgaa ccatggctcc cgccagggcg atgcatccct gcgcgcggcc attgccaaat
     1501 atctgcatgc ttaccggggc gtggtgtgcg cgcccgagca gatcgtggtg ggcgcgggca
     1561 tcgaatatct ggtgggcctg ctggcccggc tgttctgcgc cagcaccttt gcggtggaaa
     1621 atccgggcta tccccgcacg gcgcaggtgc tgcgcaacaa cggcgtgcgc acggtgttcg
     1681 tgcctgtgga cggggacggc atgacgctgg atgctctgca ggccggcggc gcgcagcttg
     1741 cgtatgtcac gcccagccac cagttcccca cgggcgcaac gatgcccatt gcccggcgca
     1801 cggaactgct gcgctgggcg ggcgcggccc cgggacgtta tgtggtggag gatgactacg
     1861 acagcgaatt ccgttacgac acccggccca ttccatcgct gcaggggctg gaccaggcgg
     1921 ggcgggtgat ctatgtgagc accttttcca agagcatggc gccgtccatg cgcatcggct
     1981 atatggtgct gccggtgcct gtgctggaga tgtaccgtgc cgaatacggc gtgtattcct
     2041 ccacggtgtc gcggttcgag cagcagacgc tgtgccgctt tatggaggaa gggcatttcg
     2101 cgcggcatct gaaccgcctg cgcagcgcct gccgcgcccg ccgggacgcg ctgctggagg
     2161 cgctgtacgc cgctttcggc cgggatggcg tggcggtgcg gggcagccac accgggctgc
     2221 acctgctggc cgcggtgcac aacggcatga cggaacggga gctggtggag gcggcggcgc
     2281 gggccggcgt gcatgtgaac ggcctttccg cctattatat ggagcacccg gaggactgtc
     2341 cgcccgccac cgtggtgctg ggctattccg atatggacga gagggcgctg cgggccgctg
     2401 tggacgcgct gcgctgcgcg tggggcgcgc ggccttcgga ataaaaagca aaccgccgtt
     2461 tccgccggac ttcgtgtccg gggaaacggc ggcttgttca tttgtatccg tacggttcag
     2521 atgcctgcgg gcagagcgcc gcccgtctgc ggggcgcttt gcgcgtctgc gtccggcgcg
     2581 gcggggagcg ggagtgtttc cggctgctcg ggctggttcg tgtcgggcag cgttaccgtg
     2641 gccttgaaca ggtcgccgtc cactgtgatg gagaacgtgc cgccctgcag ttcggcaaag
     2701 ctcttggcaa tggcaaggcc caggccgctg ccctcggtgt tgcgggaacg gtcgccccgc
     2761 acaaagcggt cggtgatctc ttccgcgctg aaatccagct cggtggcgga aatgtttttg
     2821 aacaccacct gcgcgccgtg ctcctgcggc atcagcgtga tataagcccg ggtgccgggc
     2881 atgccgtatt tggtgatgtt caccaccagg ttctcaaaga tgcggcaggt cttttgcccg
     2941 tccagcacca gaggcatgcg ttcctccggg agctgccagc ggaaatcgat gccgctggct
     3001 tcggttttgt cggccagctc gtactggacc tgcttgagca gtgcggccag atctacttca
     3061 gcgtaatgca tggccacgtt ccgtgtggcg gccttgctca cctcaaacag gtcctcgatc
     3121 agccgcttga gccgctggga ttttttgtcc agcgtttcaa tgtatttggc ccggtcttcg
     3181 gcagaaaggg cggggtcctt gagcaggtcc acataggtga tgatggcggt cagcggcgtt
     3241 ttcaggtcgt gtgagacgtt ggtgatcagc tcggtcttca tgttctggct gcgcacttcg
     3301 gcggcgacgg ccttttcaaa gcccgcgcgc acctggttca attcgtcctt cagcgggtcg
     3361 aacaggccca tgtctccctc cacaggcgtg gccaggtctc cgccggccat gcgccgggcg
     3421 gcggccagca gggccgcgta gtcctgctgg atgcggttgg cacggcggcg cagcaggaag
     3481 aacagcacca ccgtgtagat cacggttcca gcaatgccca ggaaccagaa gcagcagatg
     3541 aacgtgacga tggcgaaatt tacggccagc aggcgcagca gcgttttttc agcgggatcc
     3601 cgcaggtcga tggcggtgag ggcgcgccac atgcgcccca gcgtgccgct cacggcacgc
     3661 cagacgcgcc ccgcgccccg cagcaaagcg cgcacggccc gtgcggccag gctgcggttt
     3721 ttcagatagc ccagccaacc gtcccgggcc aggccctggc gcagcgcgag aacagacata
     3781 aacgtcacct ccaaaaacag gaacagaagc cccgcgcaca gcaggttcgc catggtgttg
     3841 gccgcggagg gcaggtccac gctgaattcg tcccgcagca cggcggcgat gctgccgtcg
     3901 aaattgcttg ttacagcgct ggaggggacg ccgttcatca gcaccgcgca gccgagggcc
     3961 agcagtacca gctccagcgg cacacggcac agccagccgc cgcccagccc gctgccgcgc
     4021 cggcggccga accacagggc cagaagcgcc gcaagggcca atgcggccgc aaacagggga
     4081 tcgaaggcgt ggctgatgaa ggagcgctcc tgagacgttg cgatatacca gctgatgtca
     4141 tccgaagccg cgaatacccg cgggaccccg tagacgaagg tcacgctttt gggcgcggaa
     4201 agatcgtagt aggacagaac gtccaggtca agatgccggc gtatggcgtc ggtgccgtag
     4261 gcctgcatat ccgtgttcca gatagaagcg gaaaagtcct gctggccctc ctcgcccaga
     4321 taggaggctg cataggacat attgccctgc gcgtcgaagc ggagcgccag cacataacgc
     4381 cactgggcgg cgtcgaagcc gtttttggcg gggtcgtccg aaagaaagcc gccaagcccg
     4441 gcttccgtgt tggtgtatgt tttgcccgtt gcgtcgtccg tcacggcgta aaacagattg
     4501 ccccagctgc ccgccagctc gtcggcatgg gctttcacgc tggcgttgaa agcgtccagc
     4561 gcggagatgg catcgggcgc gtaaacgtcc agtccgccgt aggaggcgtt ggtgccgccg
     4621 ctttcgtcac cggccgcgcc ggtgtagccc agcacgaagt cctccccggc ctcccgtgcg
     4681 gcgtaaacag cggcgttcgc gtcgctcagc aaaagctccg acggctcaaa ctccttgccc
     4741 tcccactgct cgcgggcccg ccagtacagc gtatggttgg ccgtctggat gttttccaca
     4801 aagggatcgt aataataaaa caggacgccc tgttcctgag cgcgggcgta cagcgggcgc
     4861 agcccggcca gaagccccac ggccagcgcc agcagcaaaa gtgcggaaag cagccccgcg
     4921 gccgccttac ttttcgatct tgtacccaat gccccacacc actttcaaat atttcggttc
     4981 cctgggattg tattctatct tttcacggat gttgcggatg tgcaccatga tggtatccgt
     5041 gctgacggcg cgttcgttcc aaacgcgctc gtatatctcc tcggcggaga acacgcgccc
     5101 agggtttttc atcagcagcg ccagaatttt gaactccagc ggcgtcagct ttacgggcgt
     5161 gccgtccacg gtcagctccg cggtgtcctc gcacagctcc agcccgccga tgacataggt
     5221 gcgcagggcg ggcgcagcgg ctgcgcccgt cgcccgcaga aatttttcgt accggcgcag
     5281 gtgggagtgc acccgggcca gaagttccat gggcacgaaa ggcttggtga cgtaatcgtc
     5341 cgcaccgatg ttcagcccgg tgatcttatc aatgtcctcg gatttggccg acagcatgat
     5401 gacggggaac tcgcagccct cgtcccgcag cttcatcacc atggtgatgc cgtccatcac
     5461 gggcatcatc acatccacaa cggccagatg gatgggcagc tgccgggcaa gctccagccc
     5521 ctgggcgccg ttggccgcac cgtgtacggt atagccctgg tttttcaggt aaatggcgac
     5581 gccctcgcgg atctcggcgt cgtcctccac aacaagaata tgcgtttcca tgcgtttctc
     5641 cttttgtccg gctgcgatgc ggtatgccgc gcgcccggcg ggcggctggg cggcatcgtg
     5701 cttcgtatat cgatactatt tacttaatat agcacaaaac ccggacagag agaaagaacc
     5761 ttctgcgccc gtatgcgctc agcccaccgc cgccagctcc cgccgcagca ttttttttgc
     5821 gcggtaaagc tgggtgtgca cggtcttggg catgcggccc agctgccgtg caatggcctc
     5881 cacgggccat tcctgcatga aatacaatac cgacaccgtg cggtacggtt ccgccaggcc
     5941 ctcgatgcac tcccgcgcaa ggccggcgtc ggcgcgtgtt tcgcactcag cctccaggct
     6001 gggggcgttc tgcgcggcgg ccagcgccgg agcggtgtcg ccgggggcct cggtcacggc
     6061 catgcggcgg ctgtaggcgc ttttcagatg atcctttgcc ttgttcaccg caatgcggca
     6121 cagccagggc ttttccgcgc ccggcggaca gctttccata tgcagatagg ccgagacaaa
     6181 cgtctcctgc gaaagatcct cggccagctg atggtcgtgc accagctggc agcacaccgt
     6241 atataccagc ttttcatatt gccgtaccat cgcttcaaac gcctgttccg tcattgggaa
     6301 cccctcccgc ctttttgccc cgcccggcgc gccgggcggc ttgtcttcaa cgtgttttag
     6361 tataagaccg caatctaaaa aggcgcgggg ggaaaatcca aagaatttct aaagatttgc
     6421 tgtaaatttt ataaaaccgg gaacaaaagg ctgccggaag gcgggtaata tttctgtaaa
     6481 acagcgaaac tacttgtgat ttgcctgcgg ggatggtaca cttttctatg tgatatacta
     6541 accttaaagc cgccgccgct tgcgcggctg cgcgtgtgcg ctgcacacgc ccctgtgccg
     6601 gcgcacaggg ctttgagaac attgaaccat gcaaaaccgc agtagatgcg gttttgttgg
     6661 gggcgctttg cggcgtcatg gtacagtaag gttttttcgc gaatctgaat aagacgaggt
     6721 gcgtaccgat gctgaaaggg tataccaagg aagaaaaaag ctggatgatg tacgactggg
     6781 ccaacagcgc ccattcggtg atcgttgtca cgctgctgcc gatctttttt tcgtcggtgg
     6841 cgggctttat gcgcaacacc acgtcggcca tgagcatctg gggctatgtc accagcatcg
     6901 ccatgcttat ttcggcgctg ctggcgccgg ttatcggcgc gctgggcgat tttaaaggca
     6961 tgcgaaaaaa gatgtttttt gtttttctgg ccgtgggtgt gctggcctgc gcggggcttg
     7021 ccgttacgcc catgacgggc tttgcggaaa ataccggggt ggccgaggcc gtgggcacag
     7081 ccatcctgat cctgtatgtc atcagccaga tcggcttttc gggcgcgaac ctgtactacg
     7141 acagcttttt acccgacgtc accaccgaag agcgtatgga ccgcgtctcc accatgggct
     7201 acggcctggg ctatatcggc ggctccacca tcccgctgct catttttctt gtgatgaacg
     7261 ccgtgggcgt ggatatgctg tactgcctgt ctttcatctt cggctttacg gccgtgtggt
     7321 gggccgcgtt cagttggccg ctgctgaaaa acgtggagca gaaaagctat cagccccacg
     7381 aaaagggcgc cattcgcaag accctgcggg ggctgtgggc cacgctgaag gacatcttcg
     7441 gccataaatc catttttgtg ttcatgctcg cctatttctt ttacatcgac ggcgtgggca
     7501 ccatcatcca catgagcacc tcctacggcg cccagctggg tctgggcgac accgagatga
     7561 tgcttgcgct gctgctggtg cagatcctag gcctgccgtt ctgcctgatg tacatcaagc
     7621 tggccggtaa attcagcgcg cgcgtgatgg tgggcgtggg aatctgtatc tacatgggca
     7681 tttgcgtgtt cggctttttt gtgcgcgaga cctggcagtt ctgggtgctg gccattctgg
     7741 tggctaccag tcagggcggc atccaggcgc tgtcccgcag tatgttcgga aaaatgcttc
     7801 cggacaagaa ccgttcgggc gagtacttcg gctttttcga tatcttcggc aagttttcgt
     7861 ccgtcatggg cccggcgctg atgggcttcg tgacgcagat cgccaccgtg gtgctgctgg
     7921 gcaggcagag ccttacgccg gagacggccc ccgaggccgt gttggccgcc gcgcagcagg
     7981 aggcatcgcc ctggggcgtg ttgtcagttc tgctcatctt cctggtgggc gcggtgcttt
     8041 acttcggcgt gctgccccgc atgctgaaaa aagagggcgc ggagaagtaa aaaatccgta
     8101 ctgcccatgt gcgggagggg cggggccccg cccgaagacc ccaacgcgaa aggttttgat
     8161 cgatatgccc gaagcatata cacatatccg catcgcccgc gcggcgctgg cacaaagcgg
     8221 ggagcatgcg cccagtacgg cggcctatga aatgggcgcc aacgggccgg acccgctgtt
     8281 tgcttaccgt gtgctttccg ccaaacggcc ctggccgctg ccggaactgg gcggccgcat
     8341 gcacgacgaa caatgcggcc ggttcctgcg cacaatgatc ttcgcggcct ccacgcccgc
     8401 ccagcgcagc tatgcgctgg gctttctggc gcattacgcg gcggattccg tgatgcatcc
     8461 gtatgtggcg ttccagtgcg ggccgcaggg acagttcaac atccccgagg ggcacggctt
     8521 ctgcgaggtg gccatggaca gtatgttcca tgagcaggac tgcggctcac ccgccgttcc
     8581 ggcgggaact gccgctccgg ggctgccgcc tcaggagctg gccgaagtga cgcaaatgct
     8641 tcatgacgcg gtggcgcagg tctaccgcat ggacatcccg catgaggcgc tggcggattc
     8701 cttccatgat ttccacgccc tgcacaggct gttcctgtcg cctcacggcg gcaagcgggc
     8761 gctggtctgg gcggcggaac ggctggtgct gcacaagccc ggctacgggc tttcccacat
     8821 gacgcccgcc cggcgcccgg cggacggctt tgcggagcat tgggaaaacc cctatacgca
     8881 tgcacagatg gacgcgggcc ccgaggcgct gtgcggggaa tcggcccggc tggctgcagg
     8941 atacctgaag gcagcggccg cttactggga gggccgcagc accaaggagg cgctggccgc
     9001 ggctgtgggc gaccgcagct actccacagg cctgctcagt gaaccggccc cggccggggt
     9061 gtgaaggccc gccctgcccg ggaaataaaa ttggactgca aacgcgccgc ggcgaaagat
     9121 atgcc
//
LOCUS       DAWBSB010000079         9037 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1686_length_9037_cov_3.354598, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000079 DAWBSB010000000
VERSION     DAWBSB010000079.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 9037)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9037)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9037
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1686_length_9037_cov_3.354598"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2484..2583
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4691..4700
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1860 a         2527 c         2870 g         1670 t
ORIGIN      
        1 cgattatgtt tacatcgatt gcttcaaaca ggacgtggaa gccccggagc tgctggctcg
       61 cacgctctcc gaccggcaca agggcgttgg cggagacggc gtcatcctca tctgtccctc
      121 tgaaacggcg gatgcaaaga tgcgcatgtt caacgcggac ggcagcgagg gcaggatgtg
      181 cggaaacggc gtacgctgtg tggcggaata tctgttccgc cacggatatg cctcgggcac
      241 ggcggcagat gtagaaacgc tttcgggcgt gaagcacatc gtccgcagag agccgggcct
      301 gctgaccgta gacatgggcg cgccggaact tgcgccggaa aaaattcccg tgacaggctt
      361 tgcaaagcca gttgtcaacg agctggtgga ggtgaatggc ggcgcggtgc gtatgacctg
      421 cgtctccatg ggcaacccgc actgtgtggt gtatgtgccg gacaccgcgg gcctgaatct
      481 gcctgtgctg ggcggcgcaa tggaacacag caaatggttc cccgagggtg tgaatacgga
      541 atttattcag attttggatg aaacacattt gaagatgcgg gtgtgggagc gcggcagcgg
      601 cgagaccatg gcctgcggca caggcgcgtg cgcggccgtg gtggcaagcg tgctgaacgg
      661 ccattgcaaa aaggatacgg atgtggacgt gctgctgctg ggcggaacgc ttaccatccg
      721 ttacacgggt gataccgtgc tgatgacggg caatgccgtc gaggtgttca ccggaacggt
      781 ggaggtgtaa aaacgcatca gaaagcggcg cgcggttggg cgcgcgcccg agggaagaga
      841 aaagggaaag aggcaaaagc gtatgacgaa gtatattttt gtgacgggcg gcgtggtgtc
      901 cggcctgggc aagggcatca cggcggcctc gcttggccgc cttttgaaaa gccgcggtct
      961 gaaggtggcg gcccagaagc tggacccgta tatcaatgta gaccccggca ccatgagccc
     1021 ctatcagcac ggcgaggtat tcgtgaccga ggacggcgcg gagaccgacc tggacctggg
     1081 gcattatgaa cgctttatcg acgaggatct gaacaagtac tccaacctca ctacgggaaa
     1141 ggtatactgg aacgtgctca ccaaagaacg ccgcggcgaa tatttgggcg agacggtgca
     1201 ggtcattccc catatcacgg gcgagatcaa aagctttatc tattccgtgg ggaaaaagac
     1261 ggatgccgac attgttatca cggagatcgg cggcaccacg ggcgacattg aatccacccc
     1321 gtttctggag gccatccgcc aggtaagcat cgaggccggg cgggaaaact gtctgttcat
     1381 ccacgtcacg ctggtgccgt acatcaaatc cagcggcgag cacaagacaa agcccacaca
     1441 gcattccgtc aaggaactgc agagcgtggg catcatgccg aatatcatca tcgcccgcgt
     1501 ggacgagccg ctggaggaag ccaaccggca gaagatcgca ctgttctgca acgtcaaacc
     1561 ggactgcgtc atcgagaacc tgacggtgcc cgtgctgcag gaggccccgc tgatgctgga
     1621 gcgcagccac ctgtccgata tcgtgtgccg cgagctgcat ctggactgcc ccccctgcga
     1681 cctgaccgac tggaacgaga tgctggcccg catcgccgcg cgcaggcgca gcgtgaacat
     1741 cgccatcgtg ggcaagtaca ccaagctgca cgatgcgtat ctttcggtga tggaatccct
     1801 gtatcatgcg ggctacgaga cgggcgcggt ggtgcacatc aaatgggtgg aggccgagga
     1861 ggtgaaccca tacactgcgg acgatatcct gcgggagtgc gacggcattc tggttcccgg
     1921 cggctttgga gaccgtggga tcgagggtat ggtccatgcc gcccattatg cccgcacgca
     1981 gggcaagccc tatttcggca tctgtctggg catgcagatc gctgtcatcg agtatgcacg
     2041 cagcatgctg ggctatgcgg acgcaaactc ctctgaattc gatcccgtgg gcgcgcattc
     2101 tgtcatcgac cttatgcccg accagaaggc tctggacgaa aaaggcggca ccatgcgcct
     2161 ggggcgttac tcctgtgtta tcatgccggg cacgcgaatg gcgcaggctt acggtgcaga
     2221 ggaggtcagc gagcggcacc gccaccgcta tgagttcaac aatgagtacc gcaccgcgct
     2281 gcgcgaagca ggcctcgtgc tggcgggcac ctcgccggac ggccggctgg tggaggctgt
     2341 ggaggtgccg ggcaaacggt tttatgtagg cgtgcagtac catcccgagt tcaaaagccg
     2401 tcccaaccgc gcccatccgc tgttccgcgc ctttgtggcg gacgcgctgc gttaccagga
     2461 cgaggacaaa taaacgcgct tacnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnncgcggca ggcgcgcttt tgttgtagcc gcaggcgcgg tgtcgtgtta taataccctt
     2641 aggcgggccg cccggcgggc ggctctttcc agatcaagag gcagggggaa atacgaattg
     2701 aagatcaatg aaaactacaa gaacctttcc gaaagctatc tgttttccac catcgcaaag
     2761 aaggtggcgg tctatacgca gcagcatccg gaacaggaga tcatccgcct tggcatcggc
     2821 gacgtgacgc tgccgctggc tgcgcctgtg gtggatgcgc tgcacgccgc tgtggaggag
     2881 atgggcaaaa aagaaacctt tcacggctat ggccccgagc agggctacgg ctttttgaag
     2941 caggctctga tgcgttatta cgcccggcgc ggcgttgtgc tggcggagaa cgagatcttt
     3001 gtgggcgacg gtgcgaaaag cgacatcggg aatatcctgg acctgttcga tgtagacaat
     3061 accgtccttg tgcccgaccc cgtctatcct gtctatgtgg ataccaatgt gatggccggg
     3121 cggcgtatcg tgtatgcggc ggccacgcgg gaaaacggtt ttctgcccat gccgcaggca
     3181 gatgtacagg cggacctgat ttacctgtgc agccccaata accccacggg cgcggcgtac
     3241 agccgggatc agcttcaggc gtgggtggat tatgcgaatg agcgcggcgc ggtgctgctg
     3301 ttcgacgcgg cttatgagag cttcatcaca gacggggacg tgccccacag catctacgag
     3361 gtaaacggcg ccgagacctg cgccatcgaa ttctgttcgt tttccaaaac ggcgggcttt
     3421 acggggacgc ggtgcagcta taccgtagtg ccgcaggcgc tggtgcgcgg cagcctgcac
     3481 ctcaatgcca tgtggctgcg ccgccagacc acgaaataca acggcgtacc gtacattgtg
     3541 cagcgggcgg ctgcggccgt gttcacggag gaggggcaaa aagccaccag agcagccatc
     3601 gactactacc gggccaatgc tgccgtcatc gcggccgcgc tggacgaggc cggcatatgg
     3661 tactgcggag gcaaaaacag cccttatatc tggatgcagt gccccggcgg catgaagtcc
     3721 tgggatttct tcgatcatct gctggagcat gcgggcgttg tgggtacgcc gggcgcgggc
     3781 tttggcgcac agggtgaggg ttacttccgc ctgacgggct tcggcgacgc ggaaaagacc
     3841 aggctggcag cgcagaagct gaaagaggca gtgcgggtgc tgtaaggcgc gccgtatcaa
     3901 caaaaacagt acggcaaaag caggccccgc agcggagcgt ttccgccgcg gggtctttgt
     3961 tcatacgggg ggcggcgcgt cccgccgcac atataccagc tccaccacgc cgttgcacac
     4021 cgtatgcctc gtcagacgca gaggcaatgc gggcccctcc gggggaaaca ggcggacgcc
     4081 tccgcccagg atcaccggca tgaccgtgag ctggtaaacg tcgatgaggt caagcgccat
     4141 catccggcgg gcgagctgcg cgccgccgca cacccagata ttccgcccgg agcgcgcttt
     4201 caggcggcgt accgcctctg ccgggtcctc ggacgtaaaa acggcgtttg cgtccggcgg
     4261 gggtgcaccg tgtgtgaaca cttggcactc cattcccaca tacggccacg cgccgggaga
     4321 gagctcctgc gtaatttggc ggtaagtcgt catgcccatc agtacggtgt ccacttcggc
     4381 ggaaaaggcg gcataaccat agtccccttc aaaggcttcg ccgttgttgg cgaggtccat
     4441 ccagccgacg ccgccgtttt tgtcggcaat gaagccgtca agactcatgg cgagatataa
     4501 gatcaccttt cgttccataa accgtcctcc caagaatgga tgctatttat catatcatgt
     4561 gcacggatac aagcgcaagc gggaagatat gcgttgcctt tcgcggcatt tcgcggtaaa
     4621 ataaaataac agaaagaaag cgcccgccgc gcgcgggaaa atgtgggagg gaacatacga
     4681 tgtctatcac nnnnnnnnnn gacgcaaaac ggcgaggaga tttaccagta tattctggaa
     4741 aacgccgcgg gcatgcgtgt ggctgtcatc gagtacggct gcgtcatcac caatatatgg
     4801 gtaaaggaca acaccggtga gctgcgtgac gtggcgctgg gctacagtaa gctggaggat
     4861 tacgaattgg gcggcgtatt cggcgcgttc atcggccgct atgccaaccg cattgagaaa
     4921 tcgcgcttca cattggatgg caaaacatat gaattggaac ccaacaacgg gccgaaccat
     4981 ctgcacggca catttgccaa gaaagtgtac aagggcagca tcgtggacgg cggcctggtg
     5041 ctgcgggcag tgagtcccga cggagaggac ggctttccgg gaaacctgga gcttacggtg
     5101 cgctatacgc ttacagacga aaacgcgttg gtgctggact atacggcaac gaccgacgct
     5161 cccaccatca tcaatctgac gaatcacagt tatttcaacc tggaggggca atccagcggc
     5221 agcatgctgg aacagacgtt gtgtctgaac gcttccacgt ttacagaggg caacgccgag
     5281 acctgcccca ccgggcgcat cctgcccgtg gcgggcaccc ccatggattt cacccgggcc
     5341 aagcccatcg gccgggacat tcaggcggag tacagccagc tgcagatgtg cggcgggtac
     5401 gaccacaact tcattctgga caaggaaccg gggatgctgg ctctggcagc cgtggcccgc
     5461 tcggaaaaga cgggcatcac gatggagacc tacaccacac agcccggcgt gcagctgtac
     5521 acgagcaact cactgcaaaa cgatacgagg cccggtaaga cgggcggtat atatggccgg
     5581 tatcagggct tttgcctgga gacccagcat tatccctgta cgccctcgca ccccgaattt
     5641 ccctctgtgg agctgcgccc cggcgaggaa tatcacgaaa cgacgattta taaattcctg
     5701 acggagtgaa agaatggcgg tcctgcataa aaaacgcaac gcgaatacaa aaaaacgcgc
     5761 ccggctgatg ctgggcatgg cagcctgtgt gcttgcgccc acggcgctgg ctgtgtggta
     5821 tctgacagct ctgctggcaa agcggcagcc tcctgccgcg ctggcctatg gagcgctggc
     5881 tgcggccgcg gcgggcgcac tgtgtttccg tgtgttgtac acccgcagcg cagtcctgaa
     5941 aagtggtatc gacggcgagc ggcaggccgc ggctgcgctg cgcgcgctgc cgtacgtcta
     6001 ccatgtgttg gtgaacccgg tgttccgcgt gcggggcaag gtgatggagc tggatgcggt
     6061 ggtcgtgggc aaaaacggcg tgtttatcgt agagacaaaa aaccacgccg gcgtcatcac
     6121 aggtaagacg gacgcggagt ggtggtcgca ggtcaagcgc cgaggcgcaa aaacaatgaa
     6181 aaatccactg ctgcaggcag aacgccagca caagctgatg gagcagcttc ttgccgatgc
     6241 aaagcaggcg tgccctgtgc acagctttgt atattttgcc aacaagaatg cgcgtgtggg
     6301 cgtgcgcgac gcgcgcatct ataccgatga gacctcgttg cgccgcgcca ttcagagcgc
     6361 gccggagccg gggcgcccgg tggacgcggc acggctggct gccctgctgg agcaggcctg
     6421 cgaatagtat tttttgcgcg tttccgtatc ttggtcgccc cgcggctcat tttgagccgc
     6481 ggggcttttt ggtggaaaaa caaaggtgac aacacgcccg atgcaaaaat tttgtcaggt
     6541 ttggcccggc cgatgtgtct tatagatgga aagggggaac gcggggtgca gaacacggaa
     6601 caaaacgccg tgctgctgcg gctggcgcgg gaatacggcg acagcgtggt gcgcatgtgc
     6661 tgcgtgtatc tcaaagatta tcatttggcc caggacgtgg cgcaggagac ttttttgcag
     6721 gccgcacgca aatacggtgc gctggcgcgg acagattcgg aaaaggcgtg gcttatgcgc
     6781 atcgctgtga accgctgtaa aaacgagctg cgcacccagt ggttccgccg ggcgcaggag
     6841 ctgccggagc cgtccggggc ggacccgtac ccggaggcgg atacacatat ggcgctgatc
     6901 cgggccatcg ggcggctgcc gcaaaaatac cgtgccgccg tgctgctgta ttattatcag
     6961 gatatgaccg ccgccgagac ggccgcggcg cttggcatac gcgaggacgc tgtgtatcag
     7021 cggctccggc gcgcgcggca gcagttgaaa aaagaattgg aggagcagtg aagatggaaa
     7081 agatcagaca ggcgatcgac acaaggctgg ccggacagca tcttccggaa acggtatacg
     7141 atgcgctttg cgcccggcct gcccgccgga gccgcgtgcg ccgggcagcg gccctgctgg
     7201 cggctgtatg cgtgctgggc ggcgcaacag cgctggcagc ggcccgactg ttcggcatga
     7261 acgtcaatgg acgggagctg ccgccgctgg agcccatgac catggtggaa actgcggata
     7321 caggcggccc ggcggacggt acggagcagc aggcggaggg actggacgcg ctttcccggg
     7381 ctttgggcgt gccgttcctc tgggcggagg gcgcccggga aaatccgtat acgcgcgtga
     7441 gctatcaaaa tttcggaggg gaataccaga tcgtaaaggc aaaggcgtac ctggtgggcg
     7501 acttgaggga ccttgtctgg caggacgaat acgactacta ctcctggacg gcgggcacgg
     7561 tgtaccagac gcctgtggac ctgacgctgg aatgggtctc agatgaggcg cagcagccct
     7621 ttgacacgga ttatctgggc tattatgctt acgcggacac gttcacttcc gccgggggcc
     7681 atacggtgaa cctgctggtg gatacggcgc caggcacggc cggaagcggc ctgaagccgg
     7741 agtgtgcagc ggtattcgtg gcagacggcg tgcggtatac gttatccggc catgtgacgc
     7801 agcagacgat gcgcgccatc gtggattcca tgtcttatgc atgacagaag aaataccgtg
     7861 tgaagcgaga aagccccgcg tccgtccgga cgcggggctt tttatacggc acagcttatt
     7921 cgaggatgct tttgcccgtc atctcttcgg gctggggcag gcccatgatc ttgagcatcg
     7981 tgggggcgat gtccgccaga acgccgcctt cgcgcagctt tgcattttcc acgcccgcaa
     8041 ccaggaaagg cacgggattc gtggtgtgtg cggtgaaggg gtgctcgggg tccggatcgt
     8101 acattttgtc ggcgttgccg tggtctgccg tcagcagcac cgcgccgccc ttggccaaaa
     8161 ccgctccgat cactttgccc gcacaggcgt ccacagcctc cacggccttg acggctgctt
     8221 cgaacacgcc ggtatggccc accatatcgc agtttgcaaa gttcaggatg atgacgtcgt
     8281 attggtcgct ttcgatacgc ttgacgcatt cgtcagccac cagatatgcg ctcatctcgg
     8341 gctgcaggtc gtaggtggcc accttggggc tgggaatcag cgcgcggtcc tcaccttcaa
     8401 acggggtctc cacgccgccg ttgaagaaga atgtcacatg ggcatatttt tccgtctcag
     8461 caatgcgcag ctgcgttttg ccgtttttgg ccagatattc gcccataacg ttgttcaggc
     8521 tctgcggatg gaacgcgact tccacattgg gcatcgtggc gtcatactgg gtgaagcaca
     8581 catagtacag agggaaacgg ccgttccggc gctcgaagcc tgcaaatgca tcatctacaa
     8641 aggtgcgggt gatctcccgc gcgcggtccg ggcggaaatt gaagaacacc acgctgtccc
     8701 cggcggatac gcggccgcct tcacaagtca cggcgggcac gacgaattcg tccgtcacgc
     8761 cgtccgcata gctttttttc atcacttctt ccggtgtgcc gtgcacgcct tcgccgtata
     8821 ccatggcggc gtaggccttt tccacacggt cccagcggtt gtcgcggtcc atggcgtaat
     8881 agcggcccgt gacggtggcg atcttgccca cgccgatctc ggccattttg gcttccagct
     8941 cttcaatgaa ccccttgccg gaatcgggcg gcacatcgcg gccgtccata atggcatgga
     9001 cgtaaacatc ctttacgccg ccgcgctttg ccagctc
//
LOCUS       DAWBSB010000080         8871 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1712_length_8871_cov_3.499773, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000080 DAWBSB010000000
VERSION     DAWBSB010000080.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 8871)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8871)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8871
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1712_length_8871_cov_3.499773"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1326..1335
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2973..2982
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1678 a         2587 c         3084 g         1502 t
ORIGIN      
        1 ggcaaaaccc atgggccggt ttgaaaaact gttttccaac cggtggtttg caatggcggt
       61 ttccgcaatg ctggccgtgc tgctttggct tgttgtcata gcgcaggagc cgacgcagac
      121 acgcaccatt actgtgtccg gtgtttccta tgattacaat tcagctttgt ataccggtgc
      181 gtctttggac attgtgaaac gcgaggatac taaggtcagc gtggtgattt cgggtgacag
      241 cagcgcgatc aatgaggtag accccgccga catcgtcgtg tatcccgatt ataccgaggt
      301 ttcccgttca ggcatgcccg gcgaatacac gctgcggctg caggcgcgcc gcgcggatac
      361 gtctttgaca aaatttgata tcgactccat ctcgcccgca tatgtggacg tgacgtttgc
      421 gcagatcggc acgcagaaat tcaccgtgga agtggaagcc agcgtggacc cggccgaggg
      481 gtattatctg ggcgaaaaac aggcgtcccc cgccgaggtg acgctgcgcg ggcctgtgga
      541 cgaactggcg cgcgtggcgc gggtggtggc ccgtgtggag agcgatgaaa agcgcgaccg
      601 cacgatgctg gacagcgcgg cgctggaatt tctggacgcg gacggccatg ccatcacaga
      661 cagcagcatc acggtgctgg agggtgaaca ggtggaggtg acgatccctg tactgaagct
      721 gaaggaggtg ccgctgaagt ttgagttcag caatgtgccg gcaggatacg accccgccga
      781 gctgaacgcc agcatctccc cggcgaccat ccgcatcgcc ggtgcggcgg atacggtgga
      841 cggcatcgaa agcgtgaacg cgggcttcat caacctggcg cgggtccagt tgggcaaggc
      901 cgagacactg aagatccagc tgagcgaagg acttgtgaat gtggatgacg tgcaggaggc
      961 cacggttact tttgatacca gcggctacgg cgagccgcgc gccatcacgg tgagcgatat
     1021 ccgcgtggtg aacgcgccct ccggtgtgga tgtgaaggtc atcaccgaaa gcatcaacaa
     1081 tgtaacgctg gtaggcgagg ccgaggagct ggcggccatc tcgccatcgg acgtcatcgc
     1141 acaggtggac gcctcagcgc agaatattac ggtaaaggga agcggacagc agcaattcag
     1201 cgcgcagatc atcgttacgg gcacaaagac ggtgttcgcc acgggggcgt actccgtgct
     1261 gtgcgacatc acggtgaagt gacgatgctg ctggtgagag atatccgcct gccgcttgcg
     1321 gcgggnnnnn nnnnnttgtg tacaccgttg ccctggaact tgccgacgag ggtgcggagc
     1381 gttcgttcga acgctttgcg ccctatgttt caatagcggc gcccgccgca tttactgtgc
     1441 ataacggcac acggccgctg gcgcattcgc cggtggtgtg cggctttggc ccggcgggac
     1501 tgtttgcggc gcttctgttg gcgcggcagg ggttccggcc catcgtgctg gagcgggggc
     1561 cggccatgga gcagcgcgca caggcggtgg agcgcttcaa cgcggcgggc ctgctggacg
     1621 aaaacgccaa tatccagttc ggcgaaggcg gcgcgggtac gttttcagac ggcaagctca
     1681 ccacacgcat caacgacccg ctgtgtgctt ttgtcacccg gacgctgctg gcgcatggcg
     1741 cgccccggga tattgcctac aagcagaagc cgcacatcgg caccgatgcg ctgcgggatg
     1801 ttattgtgtc tatccggcgg gaggtcgagg cgctgggcgg gaaggtgctt ttcaacacgc
     1861 cgctcaccgg cctgcgcgtg cgggacgggc ggcttgcggc ggtgtgcacg ccgcagggcg
     1921 agctgccctg cggcgcgctg ctgctcgcgc cggggcactc ggcccgggat acatttgaaa
     1981 tgctggacgg cgcgggtttt actctgcagg caaagccttt ttcggtgggc tttcgggccg
     2041 agcacctgca gcgccgcatt gaagaaagcc tttaccacga tgcggtgggc catccggctt
     2101 tgccgagggg tgagtaccag ctttcccagc atgtgggcgg acggtgcgtt tacaccttct
     2161 gtatgtgtcc gggcggcagc gtggtggcgg ccgccagcga aatgggccgc gcagtgacca
     2221 acggcatgag cctgcacgcg cgggatggag aaaacgccaa cgccgccgtg gtggtgagtg
     2281 tgaacgaaac ggatttcggc ggcgatgccc ggcgtgccat tgcgtttcag cgggagctgg
     2341 agacaaaggc atacgcagcg ggcggcggcg gctacgcggc gcccgccgaa aatgtgcgca
     2401 gctttttgga aggcgcaggc acgctgcacg ccggcgtggt gaagcccacc tacccgcgcg
     2461 gcgtgacagc ctgtgatctg ggcagcctgc ttcccggtga gcttacgggc gctctgcgcg
     2521 cagggctttc ggcctttgag cggaagctgc gcggctatac cgcgccggag gcggtgctca
     2581 ccgggctgga gacgcgcacg tccagccccg tgcgcctgcc gcgggatgaa aacggcgaga
     2641 gcaccgatgt gcggggcgtt tggccctgcg gcgaggggcc aggctacgcg ggcggtatca
     2701 tgtccgcggc ggtggacggc ctgcgggccg ctgcacgcgt ggcgcagaat ttttctcccg
     2761 caccttgagc gggaggctgg aacccgacgg aaaaatcgaa tatccaccat atcggaggaa
     2821 cctatgaacg acccgtacaa cggaaatgga cgcaaaagcc cggaggacga cctgggcaga
     2881 agcattgaaa aagaagtgaa ggaaaccatc ggaaccgtgg cggccgcgct gcaggacgcc
     2941 gcgcgagaag tgagtacctc gctgcgggac aannnnnnnn nngacctggg cgctgcggcc
     3001 agggacggcg tgcagggcat ccagagcgcc atcgcccagg cacagaaaaa tgctgagaag
     3061 gaaaagcggc gccgcaaacg gcgggattat gcaaaaaagg caaacggcgc gctcagcgag
     3121 gcgggcggcc tgatgctgcc tgcgggcatt ttggcggcgt gcgcgttttc tgcttttgaa
     3181 ccgggggacc cggccggggc gttgatcctg gggttcattt cgctgtggtt tttcgcaggc
     3241 gcatttggcg catttctcaa atcgcgcacg ctgcggcgta tcgccacata ccaaagcgtg
     3301 ctgggcgagc gctcgtactg cacactgcag gagctttcgg agcttacagg caaaagccag
     3361 ggagccgtcc gcaaagatct gttccgactg atcagctcgg gaaaatatga ggatgtttac
     3421 cttgcgccgg acggcacgcg cctgttttcg ggcgagacgg cgtaccggct gtatctggcc
     3481 cagcggcagg cgcagcagga agccggggcc cggcggcagg cgcagcccgc acggccggag
     3541 ccggcgggcg ttctggctga gtgccgcgca tttttgagcg atctgcgtga acagcagcgc
     3601 cttatcagcg acgcgcccgt actggagcag gtgcagcgca tcgaggagca gacagtacag
     3661 atcatcgcct ggctggaaaa gcaccccggc ggcgagagcc agatccgcag gtttgtcagc
     3721 tactacatgc ccaccacgct caaactgctg cgcacttaca acgaggtgga cccgcaggcc
     3781 gacagcagtg ctgttgccgc ggaaatacag acggatatct gcggtatcct gtacaccatc
     3841 aacaccgcgt tccagagcct gcaggacaac ctgctgaagg atactgcgat ggacgtttcc
     3901 gccgagatct cggcgctgga aacagtgctg gcccaggagg gcctgaccaa agacgagctg
     3961 ctcggccggt agggcgggcg gggaagaagg gagcgctgga gtttgggcgc gttcgttttt
     4021 atcatcattc tgttgttggt catcgtgccg cgcatgcgcc aggaaagtgt gcgccgcacg
     4081 ccgtatggaa aagagcatcg cggcacacag tatggataca gggccgccgg aaagggcggc
     4141 gcacaggtgc agcccgcgca gtacagctat cccgccgggg cacaggcgca gggcgccgca
     4201 ggcacgggcg gtgcgccgaa tccggacgct ccgggccgcc aaagcaacga tacgctgcgg
     4261 ggcaagcagc tgttgggcat gtgcgcgggc attgcgggcg gcgcttgggc cgcgctgatg
     4321 ggcgtggcgg cgctgggcgg tgtcagcgag gtgatctggt gcatcagcaa cggtttttcc
     4381 tttggagatg agctgatgct ggttgtgatc cccgcgcttt tggccgcggg aggctggaag
     4441 gccgcacagg ccggctttgc cctgatgaac cggatcaagc ggttccggct gtatgatgcg
     4501 atcatcggcg agcgccgcgt gtgcccggta tccgatattg cgcgtgccag cggccgcaag
     4561 cccgaggaag tggaacgcga cctgctcgac atggtgcggc gcggctattt tccgctgggc
     4621 tttgtggacg ccgagaccgc ctgcttttat gccgacaatg ccgcctggcg cgcacagaac
     4681 cccgagcgtg cccgccagca ggacgctccg ctgggcaaag agcagccccg gcccgcgccc
     4741 gccgcgcagg agcctgtccc ggaggatgag cccggcggcg gatatttgcg ggagctggag
     4801 cgccagcttg cacgcattga aaacgccgac atccgcgcca aggcggcgcg gctgcgcgag
     4861 cacaccgagg atatctttgc ctgggtgaag ctgcacccag agtgcgccga cgacgcgcgg
     4921 cgcttttgca gctattatct gcccacagcc gtcaagctgc tgaataccta caatgaggtg
     4981 gcgccgcatg ccgatgaaag cagcgtggcg gcgggcatcc agagcgaggt atcccaggtg
     5041 ctggacacta tgtccaccgc cttccggggc ctgatggaca atcttttgca gaacaccgcc
     5101 gtggatatgc aggccgagat ctcggcgctg gagacggtgc tggcccagga ggggctggcg
     5161 caaaacggct ttacgatgca gaaatgagac gcgccgtttg tccggcggcc gcggctgtgt
     5221 aattttcaga accgcctgtc cggggaaggc cccggaaaaa aaccgtgatt ttttgtgaga
     5281 aggagaccca ttccatgttt taccgtccct ggcagaccgt ccgccccgac ccagcccgct
     5341 ccgccgcgct cagcgcggcg ctgggcatgc cgcgccttgt gtgcgacgtg atgtgcgccc
     5401 gtgggctgga cacacccgaa gcggcgcgcg ccgtgtgcgg cggcggggac gctttgtctg
     5461 acccgatgct cctttccggc atggaggcgg ccgtggcacg catccaccgc gccatcgacg
     5521 acggcgagcg catcgccgta ttcggggatt acgatgtgga cggcgtgaca gccaccgcgt
     5581 tgctgtatac ctatctggat tccgccggtg cggacgttta ttacaagctg cccagccggg
     5641 atgacgacgg ctatggcctt tccgagacgg cggtggacct gatggccgcc aagggcgtga
     5701 gcctgatcgt caccgtagac aacggcgttt cggcaaatgc ggccattgcc cacgccaaag
     5761 agcagggcgt ggacgtggtg gtgacggacc atcatctgcc gccgcagacg ctgccggagg
     5821 ctgcggccat cgtgaatccc cagctccccg gcgacgaaag cccgtgcaag acgctctcgg
     5881 gcgcgggcgt ggccttcaag ctggctgcgg ccctggacgg ctgcacgccg gacgagatgc
     5941 tgccttttta cggggacctc gctgccattg ggacgattgc ggacattatg ctgcttgagg
     6001 gtgagaaccg gcgcatcgtc aaggcggggc tggcactttt gcagcagacg gagcgtccgg
     6061 gcatcgcggc gctcatcgag gcctgcggcc ttgggggcaa ggagctgacg gcggaaaatg
     6121 tctcttttgg gctggcgccg cgcctgaacg cggcgggccg tatggacagc gccgccgccg
     6181 cgctggaact gctgctgtgt gaggacccgg aggaggccga agcgattgtg gccgggctgg
     6241 aagcacagaa tgcggcccgg cagaaggccg agcaggatat tttgcagggc gtgctggaca
     6301 gcgttgccgc ggatgagacc tatggcgcgg accgtatcct tgtcgtgtgg ggccgaggct
     6361 atcaccaggg cgttatcggc atcgtggcct cccgcgtggt ggagcgcttc ggcaagccgg
     6421 ccatcatcgt atccgttgac gagaacggcg agggcaaggg gtccggccgc agcatcgcgg
     6481 gcgtttcgct gtacaatgcc atcgccgcct gcggagacct gctcatccgg ttcggcggac
     6541 acgcgctggc cgccgggctg tctgtgcggg aggaaaacct gcccgcgttc cgcaaggcgg
     6601 tcaacgcgtg ggccgcgcag gagcacccgg tgctcaagcg cccggcgctg cggctggacg
     6661 cgccgctggc gctgtccggc ctgaaagaag aggacgtggc cgcgctgtcg gtgctcgcgc
     6721 cgtttggcca cggcaacccc acgccggtat ttttggtgga aaacgctgtg atcgacgcag
     6781 tatatcccct cagcgagggc aagcacacgc gcctgcgcct gaagcagggc ggcggagtgc
     6841 tgtatgcggc ggtgttcggc caggggccgg gcgcgctggg ttacaatgtg ggtgacgcgg
     6901 tggacgcggc ggtgtgcctt tcggtgttcg agggcaagaa cgggccgatg atctccgcgc
     6961 gcatcaagga gctgcgcccg gccgggctgg acgaggcata cctggagggc acggagctgt
     7021 atgaggcgct gatgtgcggc gcgccgctga cagagagcca gcggcgggcg ctgctgcccg
     7081 cccgtgcgga caccgtggcg ctgtaccgcg ccgtgggcgc tgcccgcgac ggcgtgccgg
     7141 cgggcgacct gcgcccgctg tttgccaagc tgggcgcgcg gggcgcaggt aaggcgcttg
     7201 tctcgctgga ggcgctgcgt gagctgagcc tgctggagca gcgcgaaacg cctcagggcg
     7261 cacggtggtg cctggtgccc gcggcgggca aaaaggacct ggcatccgcg ccgattttac
     7321 gcagactgga ggcagcgcag tgatggaaga aaaagcgaaa aatgccgtaa agacttttga
     7381 agacctggcg caggccgtgg cggattccgg ccgcccatac gacgccgggc gcatcgaaaa
     7441 ggcttacgag atggctgcgg cggcccatgc ggggcagacg cgcctttccg gcgaaagcta
     7501 tgtcagccac cccgttgccg tggcgtgcct tgtggtggac ctgggcttgg atacggactc
     7561 cgtggtggcc gcgctgctgc atgactccgt ggaggacacc gagaccacgg cggaggacgt
     7621 gaaggctgct tttgggccgg acgtagcgct gctcgtggac ggcgtcacca agctgggacg
     7681 catcaagttt tcctcagtgg aggagcagca ggctgaaaac ctgcgcaaaa tgctgcttgc
     7741 catgagcaag gatgtgcgcg tgatgctcat caagctgtgc gaccggctgc acaacatgcg
     7801 caccggcgac ggctggcccg aacagaaacg ccgtgacaag gcgctggaaa ccatggaggt
     7861 gtatgctccc attgcccacc gtctgggtat gagcaatgta aaggaggagc tggaggaccg
     7921 cagcctgcag tatctggacc ctgtgggcta tgaggagatc acctctttcc tggcccaaaa
     7981 cggcggcgcg gattcgttca tcagcggcat tgcggaccgc atcaaggagc gcctggccga
     8041 gaacggaatg gacaaatgca ccatcaaaaa gcgcatcaag agcgtatacg gcatctaccg
     8101 caagatgtat atgcagaacc gcacgtttga ggaaatttac gatgtgtacg cggtgcgcat
     8161 cattctggac acagtgggcg aatgctacaa tgcgctgggc gtcatccacg acatgtacca
     8221 tcccatcccc agccgcttca aggactatat ctccacaccc aagcccaata tgtaccagtc
     8281 gctgcacacg acggtcatcg gacatgaggg aattcccttt gaggtgcaga tccgtacctg
     8341 ggagatggac cagaccgccg aatacggcgt ggcggcccac tggaaataca aggcgggcgt
     8401 gagcacgaaa gataagctgg acgaccgtat cgcctgggtg cgccagcttc tggaaagcca
     8461 gcgggagagc gaggatgccg gcgacctgct gcgggacatc aaaagcgagc tgctgcccga
     8521 ggaggtgttc gcttttacgc cgaaaggcga cgtcatcaat ctgcccgcgg gagcgaccgt
     8581 catcgacttc gcctacgcca tccactcagc cgtgggcaac cgcatggtgg gcgcgaaggt
     8641 gaacagccga attgtgccca tagaccacaa ggtggtgacg ggcgagatca tcgagatcat
     8701 aaccggcccc aaggataagg ggcccagccg cgactggctg aacctggttg ccaccagcga
     8761 ggccaagaac aagatccgca actggttcaa aaaggaacgc aaggaagaaa acatcaccga
     8821 gggcaaggca gctctggaca aggagatgcg ccgcaacctc atcaccatcc c
//
LOCUS       DAWBSB010000081         8866 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1716_length_8866_cov_2.547497, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000081 DAWBSB010000000
VERSION     DAWBSB010000081.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 8866)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8866)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8866
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1716_length_8866_cov_2.547497"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    8474..8483
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1911 a         2382 c         2783 g         1780 t
ORIGIN      
        1 tacgcactgc gctatggccc gcaggggtat gagccggacc tggacggcgt tgttgcggcg
       61 gtgcggcggc atgcggattc tgcgttcccg ctgcggttta tggggtttcc gtcctacact
      121 tattttatgc tgcagaaaat ggaggaacag ggaatgcggc tgcggctgca ccgtgggtcc
      181 aagatcatgc tgggcggggg atggaaacag ttttaccggc agcaggtaga caagcgcacg
      241 ctttatgcgt tggcggaaaa agtattgggc gtgggacaag agaatatcgt tgagtttttt
      301 ggggctgtgg agcatccgat cctgtactgc gcctgtccca accagcattt ccatgtgcct
      361 gtatacagcc gtgtgataat ccgggatgtg cgtacgcttg cgcccctgcc gcacgggaag
      421 ccgggcctgg tgaatttgat tacgccgatg gtaggcgcaa caccggtcgt aagcgtgctt
      481 acagacgatt tgggggtgct gcatgacggc gcctcctgtg gctgtggcat cgaaagtcct
      541 tatttggagc ttttggggcg tgtgggctta aaggaaatca agacctgtgc ggcgggtgcc
      601 gcagagcttc tgaagggggt gaacggatga tactggcgaa ggggaagctg tacggcgaag
      661 aagaacgtga agtgctgctg gcacggctgg aagatgaaat caatgagaca cgtgtccgcg
      721 cgccgctgcg ctcagaaacg gtgattgcag cgctggacgc acttgggaga catatcgaaa
      781 acggacagtt tgacgcacaa cttgctgcgc tggggctttc gcgccgcaca gatgcggtgc
      841 gtacagcgac cgccatgctg cggcgggaaa atctggaata ccgtctgcac atggaactgg
      901 gaatgcacct ggggagtgag tatccgctga cgccgccgaa cgggagcgca cggcgcatat
      961 gccgcgctgt gccgctgggc acagtgctgc atattgccgc gggcaatgca gacggcctgc
     1021 cggttctgag ccttgcggaa gggctgctca ccggtaatat taatctgctc aagctgcctc
     1081 aggcagacgg cgggctttcg gttgaagcgg ttcgatgcat gctggaaatc gaaccggaat
     1141 tggcggatta tatctatatc ttcgacacgc cttccagtga tgttgttacg ttgcaaaggc
     1201 tggcagccct atgcgacggc atcattacgt ggggaggaga tgcggctata gaggcggtgc
     1261 gccgttttgc acctgtggga gcgaaattga tcgaatgggg gcacaggctg agttttgctt
     1321 atatctccgg ctacgaggat gaacagctgg agctgcgggc cctggcggaa catatcattc
     1381 agacaaagca gctgctgtgc agctcctgcc agacaatttt tctggataca gaggacatgg
     1441 aacaggtata tgctttttgc gagaagtttc tgccatactt agaagaagcg tcacggcgtt
     1501 tcccacagga aggcatcgaa gaaacggcgg agcagacgct ccggcgctat ctggatgaga
     1561 tggtgcaagc catacaggga agagcgacgg acacacgcgt gtacagagga gaattttgcc
     1621 gccttgaagc gtgtttggat gacaatctgg tgctgtcggg attgttcggg aattgcccgg
     1681 tcaagcgtct gccggcggca cgtatgctgc cggtgctgcg cagagaaaag ggcaggctgc
     1741 agacggcggg gttaatttgt gcgccggagc gccgtttggc gctggcacag cgccttatgt
     1801 gctgcggcct tgtgcgcatt acgcgtgcgg gagatatgtc tgagagtctg tgcggagaag
     1861 cgcacgatgg ggaatatccg ctgcgccggt atgtgcgtat tgtggaggtg gagccctgaa
     1921 acacaaaagg agacgcgcca cacagcgcgt ctccttttgt ggaaaaatgg tatggtcaaa
     1981 tactcacagc gctcccacaa cgcggtgggg gacgtacagc tcttccagat aagcaacgtc
     2041 ttcagcgctc aagcggacgt ccagcgcacc ggccgcgtca tccaggtagc gggccttcgt
     2101 ggcgccgatg atgggtgagg tcacgccgcg ggcaaactgc catgcaagcg agatttgtgt
     2161 catcgtggcg gcgtgctttt gggcaagctc atggacgcgc aacacaatgc cgtagtcgct
     2221 ctcccgggtg ctgtcatatt tggaaacggc ggttttgtcc gtccggctgc gcttggtgtc
     2281 tgcgtcccat tccaaacggg agagccgccc cgccgccaac gggctgtacg gggtgcgggc
     2341 cacgttctgc cgcgtgcaaa tggggatcag ctcccgctca tcctcgcggt agagcaggtt
     2401 gtagtggttt tgcatggaaa cgaatggcgt ccagccgttg tcccgcgctg caagctgcat
     2461 gttgaagaat tgatagccgt acatcgcgga agcgcccagc gcacgtactt ttcccgcccg
     2521 tacaaggctg tccagcgcct ccatcgtctc ctcgacgggc gtgtctgcgt caaagcggtg
     2581 gatgatgtac aggtccagat agtctgtgcc cagccgctgc agcgtgtcgt cgatctcgcg
     2641 caggatggcc tcccgggaga ggtgcccctc gttgaagtag accttggagg cgaggatgac
     2701 ctggtcgcgg gggacgttgc tgcggatggc gcggcccagg tattcttcgc tggtacccgc
     2761 tgagtaggtg ttggcagtat cgaagaagtt gatgcccagc gaaagcgcgt gcttcacgat
     2821 ctcttcgctt tcagcagggt ccagcgtcca ggcgtgcatc cggctggccg ggtcgccaaa
     2881 actcatgcag cctacgcaaa ggcgtgacac cgtgagaggt gaattaccga gctttacata
     2941 ttccattgta ttttctcctt tttaacaata gggggcttcc tgttgcacag gcagcttccg
     3001 agcctttacg ccgttgtggg cagacatgcc cgcccatagc ggccttgcag gaaaagctta
     3061 tcatatggag ttcgctccaa gtcaagcgaa atcggaaaaa acacattttt gcattacagc
     3121 cggtccgttt tgcggcgggg cggaaggagt gtctgatgca ggcagcgtga accagaatac
     3181 agtgccgtgg gggttgttgt cacgcacgcc gatctggccg ccgtgggctt ccacgatgga
     3241 acggcacagg gaaagcccga ggccgagccc gcgccgtccg tcgccgcgct ttgcctgtgc
     3301 ggtgtaaaac atttcaaaca gcttttcttt ggctgcgtcc gggatgccgg ggccgtcgtc
     3361 agcgacctcc acgcgaaccg tttctccgct gcgctgtgcg ctcactgcaa tgctgctgcc
     3421 cgcaggcgta tattttatgg cgttgtccgt tagattgatg agcacctgga cgatgagctg
     3481 cgggtccatt ttggccatca gaagatcgtc ggggacagcg agcgtgacgg tgtgctccgc
     3541 gctgcggcgg tttaagtggc gcagcgcctc ctgggctact tcgtcgatga gctccggctc
     3601 cagtgtgagc tgcatggtac cgttttcgat gcgcgttacg gtgagaagat tttccacaag
     3661 gctgatgagc cacatggcgt cgtcatagat atcggtatag agctgctgtt ttttcttctc
     3721 gtccaacagc tccgggccgc ccatcaggat gctggcgttg ccggaaatac tggtgagcgg
     3781 ggtgcgcaga tcgtgggaaa cagcgcgcag cagattggcg cgcagctgct cctggcgggc
     3841 ctggatgaac agccggttac gggcctcggc agcctgctgg cgctccagag caagagcaaa
     3901 ttcattcagc atcgccaaaa gcagattgcg gtcgtaaacg tcgagcgggc ctccgaggtc
     3961 catggcgata cccgccaccg cgaacaccgt gtcctggctg cgcacggcaa gatagaggca
     4021 cttcgcgccg ggcaatgtgt ccgtgccggc gccggcgtgc ttgttgttgc ggaataccca
     4081 ctccgccacg gcctgttcgc cgggtgtgat atagggagcg gtatccgtgc cggcgtccgg
     4141 ggtgggaaag acggttgggc ggcccagcgt ctttccgtcg gccaggtaca ggcagatggt
     4201 gcgccccaaa aggcgctgca tctgccgcgc catttggcgg atgatgtcat cctgcgtttc
     4261 gctttgctgc agcttctggc tggtctccag caggacctcg gtgcggtagg ccttcaggga
     4321 ggattgccgc gcctggcgct gggcgcgcac cgtcagggtg ctggtgatga atgatgcggc
     4381 gaacatgatc gcgaaggtga cggcatagcc gggatcgttg aactggagcg tgaagcgcgg
     4441 cacggtgaaa aagaagttga acagaagcac gctgaaaaat gaagcagcgg caccgtaaat
     4501 acggctgccg gtgcaaacgg aaacaaacag taccccaaga atgaacactg taatgatatt
     4561 ggcctcgctg aggccaattg cggcgaagcc gagtgccacc gcggtggaaa ggccaagcag
     4621 gccgagcgtc ttgaacgtat ctgccggcgt cagccgctcg ggcggcgcgc ggcggtgtct
     4681 gcggtagggc ggcagattat ccggaatgat gtacacatcc acattggggg ccagcgccgt
     4741 caattgctcc accagcgtgg gccgcggcag aatgccgcgt ctgttgttgg aacgcccaat
     4801 gacgattttg gaaacgccgc tggcgcgtgc gtattcggca atttgcgcgg cgacgtcctc
     4861 gccgtaaacc gtggcgatgc gtgcgccgag ctgttcggcc aggcgcaggt tgccgcgcag
     4921 gcgggtgagg ctctcgccat tcagctcccg ggtgccggga acctcgacaa acagggcggt
     4981 gaaacggccg tgaaatgcat cggaaaggcg cgccgctgtg cggatgacac gggcattgga
     5041 gggagaggag gacagacaga tgaggatatg ttcgccggta taatagccgg cgctggcatc
     5101 cttgcctttt tccgcgcggc ggttcacacg gtcggccgtg cggcgcagcg caatttcccg
     5161 cagcgccaca aggttctctc tggtgaaaaa atggtccatg gcgcggcggg cctgaagcgg
     5221 acggtagact tttcccctgt ccagcctggc cagaaggtca tcgggttcga tgtccaccag
     5281 ctccacctga tcggcattat cgaacacact gtccggcacg cgttcgcgca ctgtaatgcc
     5341 tgtaacagcg gctacaatgt cgttcaggct ttccaaatgc tgaacattca cggtggtgta
     5401 cacatcaatg ccaagcgcca gcaattcttc gatatcctgg cagcgctttc ggtgccggca
     5461 gccctccgcg ttggtatggg caagctcgtc cacaagaaga agggcagggc ggcgtgcgat
     5521 ggcagcgtcc aggtcaaatt cgcgcagcgt cacatttttg tattccacgc acagcggagg
     5581 cagctgttcc agtccctcca gcagggcgag cgtatccgga cgcgtgtgag gctcgatgta
     5641 tccggccaca acgtcggttc ccgccgcgag tgccgcgtgt gcggcttcca gcatggcgta
     5701 ggtttttccc acgcccgcag catagccgaa gaatatcttc agtctgccgc gcacatgcgc
     5761 cgcatcggcc tcctgctgct ggatctgatg caaaatccgg tcggggtccg gccttgtgtc
     5821 ctgcatgaaa acgcctcctg tcgtgcgctt gctaaaattg gaaatgaata aactccgcaa
     5881 acaaaaagag ctcccgcaca ttacgcggcg ggagtgaaaa gcatgtgcgc tcaacaaaac
     5941 agcagcatgg gcagcgcgaa caggcatgca agggctgaaa cgccgctcag cagaagaaca
     6001 ggtatgccga tatagctgaa aaaccagaat gccgcagtgt gtgtttcaag atatgctgcg
     6061 atgctcctgc gcaacgcggc gaaagaaacc gccgcacgcc tcttttctct tttgtccggg
     6121 cgggaccatt ctgcgggaca acgtacagcc tgtgtgttca tcattgtgaa agcctcctgc
     6181 aaagggggaa aacacttgtt cccctttgca gcttcagtgt atcacaaccg ctgtgaacgt
     6241 gccgttaagg tgccggaggg cgcgcaaaaa agctgcaaag ccaaaataaa ggctttatta
     6301 agaccgcatt ccggacaatt tcagtataag acgcgaaggc tttgcgtgcc gttaagagtt
     6361 tttcgatact gttaatattg cataaggaca cagaataaaa gccgccgtga aagcttcaca
     6421 gcggcttttg gctattgcac aggattcact cagaaagggg catgcccagc ttttccgcga
     6481 tttcgtccag cttcattccg gacttctgag cctgctttac aagctcccgg atgcgctcgg
     6541 ccttgctttt gcggggcttt cgctttttag gggaaagcac ttcttttttg cgctcggcca
     6601 gttttgcggc cttttcctgc acagcggcga tcttcgcgtc aaattccgcg cacgccgcga
     6661 tttttttctc ctccagtccg cgaatgtttt cctccagttt ggaaatctgc ccgtcgatat
     6721 cggcggcgat ctgttcaggc gtcctgcgga cacgtttttg ttcagccatc tatcaacaac
     6781 tcccagcggt aaatttttct aataacaata tagcacaaga cgtttgcggg aacaagggct
     6841 tttttgacga agccatacaa aatgttactt tatgcggaac gagcgcggag cgcggaaata
     6901 caccagcacc agtgcggcgg caatgtacaa aaccgtgaat ccaagcagca ccgccgtaaa
     6961 aaagctgccg cccacctcta tctgcaggca ggcgaagcaa agcagataca tggcggcatg
     7021 gatgcccgaa aacagagggt tcttggtttt cagactttcg gaatagggct ggaagatgta
     7081 gtaaaggcac aggtggtgcg cggtgaacaa aagcgagagc aaaagaagcg cggcacagaa
     7141 cagggcaaga tccgcggtga aaataccggt gccgctgatg aggcagaagc cggccgccgc
     7201 aaggcacagc gcgcccgcta caagcccgtt gtacagggct acgcggcgca ggcgaatgcc
     7261 gaagctgcgc aggatgacct taggcttgcg gtaccaggca tagcggagca ggtctttatc
     7321 gcagttgtaa aacatggcgc ggcaggattt atccgccaca gtgatgctgt acatgatgaa
     7381 aacaaaggag gggagcatag cggtcaggtt ccggctcagg gctgcggcaa gttccgggct
     7441 ggagatgcgc agcgccgcgg ccgcggcaaa ggccagcgcc gcagcggcca ggcggtacca
     7501 cacagggcgc agcagctgcc gtcggtgcct cgcaaagaac agcgcgttca ggtaatcgta
     7561 ccctttcagg cgctggagct ttgccagcgc ttccggagaa agcgcggcgt cggcctcccg
     7621 tacctccacc tccttgaacg aggagccgga ggcggttttg agcatggaag acaaaagaaa
     7681 gttgaggtcc agcgagcgcg gcagcttgcg tgcatagccg ggatatccgg cggcgatgta
     7741 ataaaggcac agcgcacctg ccgcggcaaa ggccgatatg gatgcgggat gcagcagaaa
     7801 tgcggccagg cccatgtgcc aatcaaggcc cagcagcggc ggcgcgtatg cccccgccag
     7861 ccccacggcg atgacgagcc aggcgtaccc ggtgctgcgg acaagcacac gccccgtgcg
     7921 gtcgaacaca aacagatgga aggcttcgct catcatgcgg aaagccgcca gcagaaccca
     7981 ggcggccgcg ccctgccaca gcggcgcgcc gaaaagccat gcagccagca gaaggaaggg
     8041 aagataatag gagaaaaacg gcacataccg cagcgcaaga gaagcctgaa tatagcttcg
     8101 ggcatcggcg tgcagatatt tgagaaatgc gaccttttcc cgcgttacat tgaaaatatg
     8161 gctgtcgcca aaagcgccga ggatacagct gaggaaaaac agcatggaca ggaacagaga
     8221 gaattcctgc cctgcaaact gcgggcggct gccggccagc agaatgaccg gcaaaagcag
     8281 aaatgtaagc agataaagcg gtttgccgca caggtcgatg atctgccgga cggccaccgc
     8341 cgcagcggaa agcgcttttt ttgcacggta gcccgcgtag gccgtcggcg gcacaagacg
     8401 gccaaccatc caaagacggc tgaaataata aagaaacgcg ttcacacaaa gagcgccccg
     8461 catttttatg attnnnnnnn nnntggtcca gttggctcaa catgccctca tgcagcacgg
     8521 cgatctcatc gcaaagggct gtagcaagct gcagaatatg tgtggaaaat acgataatat
     8581 gctcgtgctt gatttggcgc agaagctgtt ttatttccag cgcgaccact acgtcaaagg
     8641 aggtgagcgg ttcgtccagc agaataacgg gcggccgcgc gatgagaaag cacagcatct
     8701 gcaccttgtt tttcataccg tgggagtacc ccttgatgag ccggtgccgg tcctctggag
     8761 cgatctgcac catgtcgaaa taatcatcgg cggagcgtcc gtcccgaata cgtgcagcat
     8821 tgacgtccat ataaaaacgc acgaattcat atccggtgag aaagtc
//
LOCUS       DAWBSB010000082         8817 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1721_length_8817_cov_3.356197, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000082 DAWBSB010000000
VERSION     DAWBSB010000082.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 8817)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8817)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8817
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1721_length_8817_cov_3.356197"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1770 a         2457 c         2862 g         1728 t
ORIGIN      
        1 atccacagag ggggattcgc tgttcgggga cgagctggaa gacgcgggcg cggaggaagc
       61 gccgcccgct gtggataacg cgccggagga tgcagcgctg ccggaagaag ccggcggcag
      121 ggaagcgccg cccgtggaag aaacgggagc tttaccgcag gcggcgagga aaaacgccag
      181 cgtcagacaa aatgcaaagg ctgttatttt tttcattgta gtcctccttt gccgtccggc
      241 agggacggcg cgttttcaat gtaaatgctg cgggagggga acgcgaaaga ggcgccctct
      301 ccggccatga tgtccatgat gcgcagattg acgtcctctt ttaccgcgcg gtattccacg
      361 atgtcggtgg ttttggtgta gaactgcacg gcaatgtcga tgctgctgtc tgcgaattcg
      421 gaaaggcgta cctgcacggt ctcgctgtgt acgccggggt ggttttccag catggcgcgc
      481 acccggttcg ttacggcttc cacggttcgc ttgggcgttc cgtaggtcag gcccagcgta
      541 aactgcgcca gccgcatttt catacgtgtc cagttggtga tgggctctcc gcacagcttg
      601 gagttgggca caacggtgag ggcattggcc agcgtgcgga tctttgtgct gcggaaggtg
      661 atgtcctcca ccgaaccctc cagagatgcg gtgctgatcc agtctcccag ttcaaagggc
      721 ttttcaaaaa taatgacaag gccgccgaaa aagttgctgg cggagtcctg ggcagccagc
      781 gcaaacgtca ggccgcccag gcccagcccg gcaatgaggc tgttgacatt gtagcccatc
      841 tcggtgacga cgagtactcc tgcgaacagc aggatcacag ccttgtaaat gcgttccaga
      901 aaccggatga cggtatcgcc cgcgcccagg tcaaagcggc tgcgcgtgcc cacaagcgcc
      961 agctctacac atttggaagc ccccagcagt ccccaggcca gcagcacgat gaaagcgata
     1021 cgcacggcgt gattctgcgc ggtgcgccat gcgtcggtgt ggatgaaatc aaagggcagc
     1081 agccgcacgc cgatgtacag gccgatgagc gcaatgaaca gagcgcacgg ccggatgaag
     1141 ctttgcagca gcgtggaaag atagggacgt tcaatttttt gcgcggctcg ggtaaggcgc
     1201 gggccgacca catgcttgaa cacacggctg gcgatgaaaa aaccggcgaa ccacgcggcg
     1261 cagataaggc actgcaccag cgtgttgctg ttttgagaaa tattttcaat ggtctccaca
     1321 gtttcggtca aaaacagtac ctccttttgc ggctccgcgc gcggatgtgc cgaacgcaaa
     1381 accaaccgtt tgagtagata atacatagta taacatattt gccgggaaat ttgtggccat
     1441 tctattgcaa tcgcgcgaaa atgcgctata atatggtaca gaaagtctgt ttcagggcgg
     1501 ggtgagattc cccactggcg gtgaaagccc gcgaccggta tatgcgcagc atgtaccgct
     1561 gatttggtga aattccaaag ccgacggtgt gcgtgccgtg tttttgcgcg gtgtgtcgag
     1621 tccggatgaa agaaacggcg gcagtgccag aggtttcgcc cttttgcgtt tcgcagaagg
     1681 gcgtttttgt tttggcggcg gcccggacac ccacgagcag gcctcgaaat tttattttgg
     1741 aggttttatc catgcgcaat gtttccgcca atacccgcaa gctcaccacg ctggccatgc
     1801 tggccgcgct ggccatcgtc ctggtggcgc tcatccattt cccgctggta cctgccgcgc
     1861 ctttccttga atatgacccc gcggacatcc ccattttcat cggaaccttt ttgttcggcc
     1921 cggccgcggg gctggcgctc acagccgtgg tatgcgtgat ccagggcgta acggtcagcg
     1981 cggccagcgg ccccatcggc atcatcatgc attttctggc cacgggcacg tttgtgcttc
     2041 tggcaggcaa tttgtaccgg gcgcatcgta cgcgcaaggc cgcgctgctg ggccttgccg
     2101 cgggcacgct ggccatgacg gccgtgatgt gtgtgtgcaa cctggtgttc acaccgctgt
     2161 ttatgggttc gcctgtgggc gaggtggtga agctgctgct gccggccatc atcccgttca
     2221 acctggccaa ggcggccatc aacagcgtca ttacgtatct tgtgtataag cccatcagcc
     2281 gtctggtgtt cggcgaaaaa gaaccggccg cctcttgaca ggcgcatggt gttctgctat
     2341 actgttatac acaaatgaat aaaccttatc aagagcgact gagggaacag gccctgtgac
     2401 gtccggcaac ctgctgtaac agcaaggtgc caaatcctgc gggtaacacc gaaagataag
     2461 ctgatgcaaa ggcccggtgt tgtctgccgg gcctttttta cgcgcttttg caggaaacaa
     2521 gaaaggaaaa caaaaatggc aagaaaactt tttacctccg aatccgtcac cgagggccat
     2581 cctgataaga tctgcgacca gatctccgac gccattctgg acgcgctgct ggaaaaagac
     2641 ccgaacagcc gtgtggcctg tgaggtgact gccagtacgg gccttgtgca tatcatgggt
     2701 gagatcacca cggaaggcta tgtggatttc cagaagattg tgcgcgacgt tgtgcgggac
     2761 atcggttata cccgcgccaa atacggcttt gacgcggaca catgcgccgt gctttccaat
     2821 gtcgacgagc agagccccga tatcgccatg ggcgtgaacc cgggcggcgc gggcgaccag
     2881 ggcatgatgt tcggctacgc ctgccgcgag acgccggagc tgatgccgct gcccatttcg
     2941 ctggcgcaca agctggcaaa gcggttgacg gacgtgcgca agaacggcac gctggactat
     3001 ctgcgcccgg acggcaagag ccaggtgact gtggaatacg agggcaacag gcccgtgcgt
     3061 gtggacgctg tggtcatttc cacccagcac gctccggagg tgaagatgga ggcgttgcgc
     3121 aaagatattc tggagcatgt gatccgccct gtcgttcccg ccgagctgct ggacgaacag
     3181 acgaaatact atgtcaaccc cacgggccgt tttgttgtgg gcgggccgca gggcgattcg
     3241 ggcctgacgg gccgcaagat catcgtggac acctacggcg gatatggccg ccacggcggc
     3301 ggtgcgttca gcggcaagga ccccacgaag gtggaccgaa gcgcggctta tgccgcgcgc
     3361 tgggtagcca aaaatgtggt ggccgcgggg ctggccgagc agtgtgaggt gcagcttgcc
     3421 tacgccatcg gagtggccga gccggtgagc atccgcgtgg acaccttcgg cacaggcgcg
     3481 gcggatgagg ctgccatcga agccgcggtg gaacaggtgt ttgatctgcg gccggcgtcc
     3541 atcatcgagc agctgggcct gcgcaggcct atttaccgtc agcttgcggc ctacggccat
     3601 atgggacgtg aggagctggg cgttgcatgg gagcagacgg ataaagcggg ggccctgcgt
     3661 gcggccatgg gcctgtaaac ggaaaatgca tcgttttccg cggcgcagcg cataaaatgc
     3721 tgaggaaata aaaaagaagg agccggaggg cagaaagggc gcgcgccgct ttttttgcct
     3781 tccggtttgt gttatactat aaaaaatagc cacggcctat ttttgggggc catgcaggcc
     3841 gtccctggcc tgcggccatt aagatgagct ttttatggct ttgctgtgca aagctataaa
     3901 atagttccgc cccgccgctg cagcggtgtc gggagcaatg aatgcatccg ccggcggagc
     3961 cgtgacaacg atgaaaaagg gagaaaacac cgtgcgttat gatatcgtga tggtgaccta
     4021 caacagcatg aaatggctgc gcgcatgtgt ggccgcgctg gccgccgtgc agtatccgct
     4081 ggaggagctg caccttgttt tcgccgacaa cggctccacc gacggcacat tggaggagct
     4141 gcggcgcatg gcaggggaat acccggcttt cggcggcttt accgtggcgg aaaacggcgc
     4201 gaaccttggg ttcggcgcgg catgcaaccg cggcgcggca aaagggagcg cgccgctgct
     4261 gttttttttg aatttggaca ccgaggtctg gcccgacgta ttcctccgcc tggacgaggc
     4321 cgccgtgcgc gcgccgcagg aaacggcggc gtttgagtgc cgtcagcttc cctatgaaac
     4381 agggcatcac atcgatcccg taacgttgga gacaagctgg gccagcggcg cggcgctggc
     4441 cgtccggcgc gccgccttca cgtctgtcgg cggttttgac gaacatctgt tcatgtactg
     4501 cgaggatgtg gacctctcct ggcggctgcg cgccggagga taccggctgc aatacgtgcc
     4561 cgcagcgcgc gtcacccatt attcctatga gaacggcggc cccaaattgg gagaatatgc
     4621 gggcagcttc tacggcaatc tgctgctgcg ttataagttc ggctcctggc gggatatctg
     4681 gcgcgggcat aaaatgtatt tgggcgcgct tcgccgtccg ctgcatttca acggtgtgcg
     4741 ccgcgttctg gcaaaaaatt atctgcgtca ttttataaag ctgtggcctt tcctgttctg
     4801 gcgtttttct caccgtgccg aatacggcgc gcgtccggca cgcttcgaag gcggcttttc
     4861 accggacaga ggcctgtgga aatttacgcc gccgcaggaa acgccgctca tttccgtcat
     4921 tgtgcgcacc tgcggcaggc cgcagacact gaagcgcacg ctggagagcc tgcgctggca
     4981 gacataccgc aattttgaga tcatcgtcgc ggaggacggc ccgcctgcgg cacgggaaat
     5041 ggtggagcgg gattttgcgg agctgcccat acgctatctg aacgatggcc ggttccacgg
     5101 ccgtgcagcc aacggcaacc gggggcttgc ggcggcgcgg ggagcgctgt gcaactttct
     5161 ggacgatgac gatttctttt atcccgatca cctggaactg ctggcgggtg tgctgtgcgc
     5221 gcacccggaa gcggacctcg tgctgggcag cgccatggcg atgttcgtgg cgaaggacgg
     5281 aaccgtcacg gaggtgcgcc ccatggtatt cgaccgcatc gaccgtttta ccatgtgtca
     5341 aaactgccgc attcctatac agaccgtgct gttccgccgc agtctgttcg agcggtacgg
     5401 cggcctcatc gaaacgctgg aagcccatga ggattggggg atgtggctga aatatctgga
     5461 gcacgggcgc cgcatcaccc cccatgcgcc ggacgtgcgc cgggccacca gccttttcgt
     5521 gcagcccgcg gcccaaagcg atgcggccgc gcgcatggag gcataccgca aaagcgacgc
     5581 ggatttttac agtgatgctt cgctgtgctt caacgtcacg ctggcggata tgcgccgcta
     5641 ttatgacgac atgatcgcgg acatgcgctg cctggaggcg cgcggtgagc tgcatgaatt
     5701 tttggagcag caggcaaagc gcggtgaata aaccgcccgc gccgcacagt ccggccctgt
     5761 ttttcatatg ctgggagtag tgtcagggaa aacggaggtg catgaaatgc tgagcggagt
     5821 gatgctgatc atcgtgacga tgttcgcggt gctgggcgcg tattatttgt cggaccttct
     5881 gacgaactgc ctgttccgcc gcagaaaagc ggcggaagcg ctggtggtgc ttcgggcgga
     5941 cacaagcgag cagatgtggg ccggcgtgct ggatgcacgc gcggctttgc cggacaccgc
     6001 ggtggtggtg ctgtgcccgc aggaggacgc tttcacgccg ccgggagcgg gaatgcgggg
     6061 tgtggccttc gctacgccgg acacactggc gcaagtggtg caggctcagc tcaaggtgca
     6121 ggaataacgc accgcgcagc tccggcgtga agcagagaag agcgcggtgc atcaaagaaa
     6181 acaaagagga aaaagaaata cccgggaaaa agccggcagc cgaaaagccg gatgcatttg
     6241 cggcgcaaag tacaagccac acggcgaatg ccgccccggg ccgtcttgta cggcgaacag
     6301 ggccggaagc gctgtccgca ggcagcatgg cggagaagcc cggctttttc gacaggcgaa
     6361 agaataaaag ggagacgcgc atggcgcagg aacaggaatg gcagaccctt tcgggcacag
     6421 ttgaaaatat cgtatactgc aacgaggaaa acggctacac ggtgctggaa ttttccagcg
     6481 ggggagacct ttataccgcg gtgggcgagc tttcggatgt gaatgtggga gaagacgtga
     6541 cgctgcacgg ccgctttgtc acacacccca gcttcggcga acaattccgc gtggaggcgt
     6601 gtgaggtgcg catgccggaa agcgcaacgg cgatccggcg ctaccttgcc agcggcgcgc
     6661 tgccgtacat cggcaaagca ctggcagggc gtatcgtgga tgttttcggc gcggatgcgc
     6721 tggaggtcat cgccagcgat cccatggcgc tgacgaagat caagggcatc acgccggaaa
     6781 aggctctggc ggcttccaac gaattcaagc gtatcttcgg cgtgcgggag gccatcggat
     6841 atctggcgcg atataacctg ccggcctccg ccgctgtcgc gctgttccgc cagtacgggc
     6901 ctgatacggt cgaggtcgtc tcccacaatc cgtacgtgct gtgcgggtat cccgcttatc
     6961 aggatttcgg cgtggcggac gccattgccc agagcatgag ccttgagtat gacgcgcggg
     7021 agcgcgtgtg cgcagggctt ttgttcgtgc tgcgccacaa tctgcagaac ggccacgcct
     7081 gcctgccgca ggacaagctg tgcggcgcgg cctccggttt tttgggcgtg gagccggaga
     7141 ctgtggcttc cacgctggcc ttcatgctgg aaaacaacga tttatgcgcc gtggaatacc
     7201 gggaaaaagc atgggtatac ctgcctgaat atatgcgggc ggagctttcc gtggcccgcc
     7261 accttcgctg gctgatcaag tatccctgcg ccgggggcga gcacgcggaa aagaccatcg
     7321 cccgcatcga agcggcgcag ggcatcacct atgcgccttt gcagcgggag gcaatcgcag
     7381 cggcgcttgg cgccaatgct ctggtactca caggcgggcc gggcacaggt aaaacgacgg
     7441 ccgtaaacgg catgctggcc gcgtttgagc aaaatgggga ccgtgtggcc ctggccgcgc
     7501 ctacaggccg tgctgcgaaa cgcctgtcgg agctgacagg ccgcaaggca aagaccatcc
     7561 atcgcctttt ggaggtggat tatacgaaca atgacaccgt gcgctttatc cacaacgaaa
     7621 aaaacctgct gaaatgcgac gttgtggtga ttgacgagat gagcatggtg gatgtgatgc
     7681 tgtttgagag cctgctgctg gcgctcaagc cgcaatgcaa gatcattatg gtgggagacg
     7741 aggaccagct gcccagcgtg ggcgccggca acgtgctggg cggcgttatc gcctccggcg
     7801 tagtgcctac gatccgtctg cgggatatct tccgccaggc ggccgagagc atgatcgtct
     7861 ccaacgcgca ccgcatcgtc acaggacagc tgcccggcaa gggctcgcgg gacagtgatt
     7921 tcttcatgct ggaatccgac ggcgaagcct gccagcagct ggtgtgcgac cttgtgtgcc
     7981 gccgcctgcc tcagagctat ggattcgacc cgttcgaaga cattcaggtg ctgtgtccgt
     8041 ctaaaattgg cccgctgggc acggttgcgc tgaacgcggc gttgcagcag cggctgaacc
     8101 cgcccgcacc gggccgcccg cagctgaata tccgcgacaa gatcctgcgt gtgggcgata
     8161 aggtaatgca ggtgcgcaac aattacgaca ttccgtatac ccggcccgac ggcggcgagg
     8221 agggcgcggg cgcgtttaac ggcgacatgg gcgtgattgt cgatgtggac gtgcgcggcg
     8281 gcagtgtgac ggtgcgcagc gaggacaggt tgctggtata caccgccgag catgtgcggg
     8341 agctggagcc ggcctatgcc gtcaccatcc acaaaagcca gggcagcgaa ttcccggctg
     8401 tcatcattcc ggtgatggac gtgccgccca agctgtgcta ccgcaatctt ctgtatacag
     8461 gcgtcacacg tgcaaaaacg ctttgtatcc ttgcgggcca tagcgcggag gtggcgcaga
     8521 tggtgcgcag cgtgcggagg aacaaacggt tttcctgtct ggccgatctg atccgggacg
     8581 aaacgctgtg aacagggggg atagctcttt gaaaaaatca aagcggctgt ttgagctgtg
     8641 cggggatatt ctgttcccgc gccgctgccc tttctgcgat gcggttttgg gcttttccgg
     8701 tccatgtgcc gtatgcgggc cggcgctggc gtgcatacgc cgtcctctaa ggcagccggt
     8761 accgaagcag gggcacgcgc tggagcatct ggatgcggcg tatgcgccgt atttcta
//
LOCUS       DAWBSB010000083         8748 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1740_length_8748_cov_3.608651, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000083 DAWBSB010000000
VERSION     DAWBSB010000083.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 8748)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8748)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8748
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1740_length_8748_cov_3.608651"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1673 a         2529 c         2929 g         1617 t
ORIGIN      
        1 acgatgttct tctccggcgg cctgatccca aactacctgc tgctcaacag ccttcacctt
       61 gtggggacgc gggcggccat gctgatgccc ttcgccatca actcgttcaa tcttatcatc
      121 cttatcaact ttttcaggag cattcccaat gagatcgagg aggcggcgct gatcgacggg
      181 ctggggtatt tcggcatcct gcggcgcatc gtgctgccgc tctccacggc ggccatcgcc
      241 accatcggcc tgtactatgc cgtgttcttc tggaacgact ggttcaacag cctgatctat
      301 ctcaagagcg accaataccc tgttatgatg ttcctgcgca atatcgtgaa cggtaccatg
      361 gtcatcggca cgggcagcgg ctcggcggaa aagacgacca ttgccatctc ggtgaaatcc
      421 gcggtcatca tcacatccac cgtacccatt atcgtgatct atcccttcct tcagaaattt
      481 ttcgtcaagg gcttgaccat tgggggcgta aagggatgag cgcgtttccg tcgtcagcgg
      541 aacccacaaa aggctatgcg agccacgagg aggcatgtcc agtggtacag atcaaaacga
      601 tcggcaccga acagcgcaac cccaatacgc tggagatcga ccgcgcggat actgcggaga
      661 tcctgcggct gatgaacgaa gaagacaaaa aagtggcgcc cgcggtggaa caggttctgc
      721 cgcaggtgga aaaagcggtg gatgtaattt acgcgcacat gcgctgcggc gggcggctca
      781 tctactgcgg gtgcggaacc tccggccgcc tgggcgtgct ggacgcatcg gaatgcccgc
      841 cgaccttcgg cgtggagccg gggctggtgc gcggtgtgat cgccggcggg ccggacgcgc
      901 tgaccaacgc ggccgagggc gcggaggacc attttgagga cggtgcaaaa gacctgcagg
      961 cgctggactt ccgtgcacag gacgtgcttg tgggcatagc ggccagcggg cgcacgccct
     1021 atgtgctggg ggcaatggac tatgcccggg cgttgggggc acccgtgatc gggctgacct
     1081 gctgccccgg ctccgccatc gatgcggccg cggatatcgg catcgcccca cagccggggc
     1141 cggaggtcgt caccggctcc acccgcctga aaagcggcac cgcacagaaa atggtgctga
     1201 atatgctgtc cactgccgtg atggtaaagc tggggaaggt atacagcaac ctgatggtgg
     1261 atgtgtgcgc cagcaaccag aagctggtgg cgcgcgcggt atccatcgtc tgctgcgcga
     1321 ccggcgtgag cccggagcgc gcgcgcggcg ttttggatga ggcgggtttt tccgccaaga
     1381 cggccatcgt tatgatcctg tgcggcgtgg atgccggcga ggcccatgcg cttttgcagc
     1441 gtgtggacgg gcaagtttcg gcagcagttt tatcgcggcc ggacggcgta cagaccgatg
     1501 cgtttgtgcg ccggctgtaa cagcaaaaag cgccgggaca gcccaaggct gtcccggcgc
     1561 aagttaagtg cggcggaagg cggcgttctc aataccggcg gcggagcggc ggagcaccgt
     1621 ccacacggtg gggacggcag ctttcccggc ttttttccag gctggccgcc gcgcggtcgt
     1681 aatccagatg cgccacggct gtgaacagaa catccaccgc caccatctgg gcgatgcggc
     1741 tgctcatggc gccgctgcgg ggcgtggcct ccggcgcgga gatatacagc cggatgtcgg
     1801 cgttctgggc cagcacgctt ttgtcgaatt tcgtcagcgc cacggtaggc gtgccgcact
     1861 ggcgggaaag ggccagcatc tccagcactt ccttggtatt gccggacatg gagatcagta
     1921 cagccacatc ctgcggcgtc atattggcgg cgtaggtgag ctggatgtgc aggtcacggc
     1981 agaagcacac gtttttgtcg atgcgcagca gcttgttgta caggtcctcg ccaaccagag
     2041 cagacgcgcc gacgccgaaa atgcgcacgg aacgggcgtt caggatttgg cgtgcggcag
     2101 cctccacgga ggcggggtcc agcagctttg tggtgtcgcg tacggcgcgg atgctgttgt
     2161 tcttgacgct gaggatcagc tggtcgatgc cggtggcttc ccgaatatcg gagaaggccg
     2221 ctgcctcggc gggctcaccc gccgtttcat gcagctcgct gaacaggctt tttttcaggt
     2281 ccttaaggcc gtcgaacccg atggctttgc agaaacgcac ccatgcgacc ttgctgacgc
     2341 cggactcctc ggccagctgg ctgatgggct tggcgaatac attttccacg ttatccagaa
     2401 aatacgaggc cacctttttt tcggcgttgc tcaggttttc gtaaatgttc tgcgtgcgta
     2461 cctcgatgtt ggtcatttca cttcttctcc ttttcgggcc gtgttcacag gctgattttc
     2521 cccatcacca cgccgtgcgc ggcgccggtg gctccgggcg cgttgttggc gcagccatac
     2581 agcgcttcgt ttgccagaac ggcaaaggca actgcctcct tggcgctgct gtccaggccg
     2641 agctgctcgt tggtaagcac ctggcagccg ggcaggcagt tggctatatg agccagcagc
     2701 acagggttca tgcttccgcc gccgcccacg atcagccgct cggggcgctg agggcagaac
     2761 cgttccacac cctcccggat gcactcggct gtaaagcgcg tcgcggtgga cagcgtgtcc
     2821 cgcagggaaa cactcagcgt ccgggcatgt gcgcacaggg cgtccacata ggcggcgccg
     2881 tatacctcgc gccccgtggt cttgggcggc ggggcgctca ggtatgggtc ctgcatcatc
     2941 caggccagca gcccggcgtt taccgtgccc tgcgcggcca gccgcccgcc gtcgtcaaag
     3001 cgggcttttc cctctgtcag ccgcgcggca agcgcgtcca tgaccatatt gcccgggccg
     3061 gtgtcaaagg caaatacgtc attcagggaa cagcctttgg gaagtacggt gatattgccg
     3121 atgccgccga tattctgcag ccccacggtg cgctgcgcgt cccgataaag caagtattcc
     3181 gtgtagggga ccaatggagc cccaagcccg ccggcagcca tgtcccgaac gcggaaatcg
     3241 ctgacgaccg gacagcccag ccgttccgca ataacggacg cctcgccggt ttgaagcgtg
     3301 ccccgcaccg ggcggccaag gtattcccgt gcgtccggcg cgtggaatac ggtttggccg
     3361 tggctgccca caaagctgac tttatcgggg gcaacgcccg cctgtgcgca cacggcctcg
     3421 caggcgtcgg cgctgagctc acccagataa aagttcagaa ggcacagttc ctccgtgccg
     3481 ccgaaatcgc ctgccgccac gcgcagaatg cgcgcccgcg ctgcgtccga gaaaggcagc
     3541 gtgagaaacg acagcagccg cacgcgggta tccgtgccgc agccgctgat ctccaccagg
     3601 gcggcgtcta tgccgtcggc agacgtaccg ctcatcagcc caatggccag gcgagggctt
     3661 gcgtcgtcat ggaaaagagc gcgcttcata aaaatgtcct cctttatatg gcccgcgccg
     3721 ggcctgaaaa agatgaaata aaattacaaa caaattgttc tttagtttca attatactcc
     3781 caacaggtgg cgccttgtca acagaatcca taaaaaattt tacgaaataa agcgtaaaat
     3841 tcatttattg caaagaaata tttcaaaaat gaaaaatagt tgcgcggctg acgcgcgaat
     3901 aacaggaaag agggaggaac cggtgcatgg tgaaatgcgg gatcgacagg atcggtgaat
     3961 tcggtgcatt gttccggggc aaaaggctgg ggatggtgac gtctgtttca ggcgtgacgc
     4021 tggacctcag gcccggatat gtgaaatttc atgaggtcta tccgctggta tgccttttcg
     4081 gccctgagca tggaatgtac ggcaaagacg gcgcggggga ggatgtggac gatgttgccc
     4141 gcgacagcgc gacgggcttg ccggtataca gcctgtacgg cggcgaggag ggcaggcata
     4201 tcgcgccgga acgtttggaa acgctggacg cggtggtcta tgacattcag gatctgggca
     4261 cgcgctttta caccttcatc tccacgctgc tctatgtcat ggaggactgc gccgcggcgg
     4321 gccgggagct gatcgtgctg gaccgtcccg caccgctgga cggcgtgacg gtggagggcg
     4381 gcctgctgga gccggaatac cgcagctttg tcggagcgta cccgctttgc atccgttacg
     4441 gccttaccgc gggcgagctg gcgtgcatgg taaatgcgga acaaaagctg ggctgccggc
     4501 tgacggtagt accctgcaca ggctgggagc gcacgcgcct gttcagcgag acgggaaacc
     4561 tctggatgat gcccagcatg ggcattccgc atttcagcac ggctctggtt tatccgggca
     4621 cctgtctgtt cgagggaaca aacgtctccg aagggagagg gaccgcatgc ccgttcgaga
     4681 tgatcggcgc gccgtttgtc gacggcgcgg cactggccgg gaccatgaac cgggagggcc
     4741 tgcccggcgt gatttttacg gcggcctgct ttacgccata cgcttcgaag cacaagggcg
     4801 agccgtgcga aggggtgctg ctgcatgtca gcgacgcaca tgctttccgc ccggtgcgca
     4861 cgggcgttac actgctgtac cgcatccggg agatgtggcc acggcagttc cggtggaaac
     4921 cacgggatgg gaagccgcgg cgcttcatca gcctgctggc gggctgcggc gcgttcgagg
     4981 acgagccgcc tgcaccggaa gcgctgcttg cggcatggga agcggacagc accgcttttg
     5041 cacggcgcaa ggcggcatat catctttaca aataaaacag gagggataca gatggatcta
     5101 cgggaacaga tcgggcagag actggtgaca ggctttccgg gaacagagct tacggaggat
     5161 ttccgcagga tggtgcggga gtacaaaata tccaatgtga tcctgttccg ggagaatatc
     5221 acggattgtg cacagctcaa acaactgtgc ggggagatcc aggcgctggt gcggcgggaa
     5281 acgggccatg gcgcctttat cacagcggac caggagggcg gcctggtgac gcgcctgccc
     5341 ggggacgcgg taaatgtgcc gggcgctatg gccatcgccg ccacgggaga cccggaaaat
     5401 gcctaccgcg ccgggctttt gacggggcgg gagctgcgcg cgctgggtgt caatttcgac
     5461 cttgccccca gtgtggacgt caattccaac ccggaaaatc cggtcatcgg tgcgcgaagc
     5521 tacggcgaca cgccgcagga ggtctcccgt tacgccaacc ggatgatacg cggcctgttg
     5581 gacggcggcg tgctgtgctc ggccaagcat tttcccggcc acggagatac ggacacggat
     5641 tcccatctgg cgctgccctg cgtggacaaa agcatggagg agctggaacg gacggagctg
     5701 ctgcccttcc gggaggcagt gcgtgcgggc gtgcccgcgg tgatgacgac acatatcctg
     5761 ttcccgcagc tcgaaccgga gcatcttccg gcaacgatgt cccggcgcat catgacgggg
     5821 ctcctgcggg agcagatggg ctttgacggc ctcatcgtca gcgactgcat ggagatgcgg
     5881 gccatcaagg accacttcgg cacggtaaac ggcgtggtgg cggccatggc tgccggagtg
     5941 gacctggtgt ttatttccca cgacacactg ctcagcgggc aggcggcccg cgcggcagag
     6001 caggccgtgg ccgagggacg gttgagcgcg gcggaaatgc aggccagcgt gcagcgcatt
     6061 ctggcgctga aggaaaaatg ggtggacggc atgcccgagg gcggcttcga ttttgatgcg
     6121 gcacgccggg agaacgaggc gctgctgcaa aagaccatca cagaggtgca ccgccccgca
     6181 ggcgggcggc ccgcactggg gagcgcgccg ctgtgcatcg gctgcccggc ctaccgcacg
     6241 tcgctggtgg ggaatgtgga cgccggcgag acgaaccgct tcggcgacgg tatggcacgg
     6301 gcgctgcatg gcagcgcggc ggccatgcag ccggagccgg ggcgggaaga gatcgcggcg
     6361 ttggtagaac tggcgcggcg gcatacctcg gccgtggtgg gcaccttcaa cgggcgtttg
     6421 cgcaggggcc agctggagct ggtggcccgg ctggcggagg cgggcgtgcc caccgtggcg
     6481 gtggcgctgc gctgcccgta cgatctggcg ggcctgcccg aaagcgtgtg gacgctggcg
     6541 gcattcgagt atacggagca gagcattcgg gcggttgcgc gcgtgctgcg cggcgaggcc
     6601 gaaccaaccg gaaggctgcc cgtgacttta tgagcggaga agaaaaaatg ccggatatga
     6661 gatatgcggc cgggatggac ggcggcggga caaaaacggt tttagaggcc cgcgcgccgg
     6721 acggacgcgt gctgcttcgg gaacggtttg gtcccctgaa ccttaacagc gctgcgtcac
     6781 aggcagtgcg ggagacgatg cagggctgta cggatgcatt ggcgcgcctg cctggcggcc
     6841 tggaggcctg tgccgcgctg tgcgtcggca gcgcgggcgt cagcaatccg caggcacgcg
     6901 cgctgctgga gcaaatgctg cgtcggtgcg gataccgggg cgcgcttctt ttgacaggag
     6961 atcaggaaac cgcgctggca ggcgcgctgg gcgcaccctg cggcatggtg cttatcgcgg
     7021 gcaccgggtc catttgtttt ggcagggatg cagcgggtaa aacagcccga agcggcggct
     7081 acgggcataa aatcgacgac gagggcagcg gctatgcgct cggcagagac gctctggcgg
     7141 ccgttgtgcg ggcgcaggac ggccgtgggc cgcggacgct gctcacggat ctggtgtttg
     7201 cacagctgaa ggtggttgac gtcggcggcc tggtgcagtt tacttatgac ccgcaaacag
     7261 acaaaaaaca gatcgcggcg ctggctccgc tggttgcccg ggcgtatgaa gccggggacg
     7321 aggccgcgaa agcggtggtg gaaaaagcat gccgcgagct ggcgctgctg gtggagccgg
     7381 tagcccgcgc tctgcatatg gaggacgggg tgctggcgct gacgggcagc attctgctgc
     7441 gggacaaagc ggtgcgcgag ggtacggcgg cgctgcttcg gatgcgattt ccgggcatgc
     7501 ggctggcgga acccaaaagc gacgccgccg caggcgcagc gcttctggcg ctggaaacat
     7561 tgaatacaga aaaaggagaa aataaaaatg cctgatatgc tggtgaaatt atacgacctg
     7621 cctgaagatg cgcagacggc gtgccctcgg gatgagagcg tacagatccg ccgcgcaatg
     7681 gcgccggata aatttcgtgt ggtggaatgg gtgaaggagc actccggcct ttccgcagca
     7741 ggggagtgcg acgtgtgctt tgcccgcccg gctgtttcgt gcttcatcgc cacgcggggc
     7801 aaaaaaatcc tgggttatgc ctgttatgat gcgacggctc cggatttctt cggccctaca
     7861 cgcgtgctgg acgaagagca gggcggcggc atcggtcggg cgctgctgct gcgcagcctc
     7921 gcggccatgc gtgcggaggg ctacgcctat gcagtcatcg gcggcgtggg cccgcaggag
     7981 ttttacaaaa agtgtgtggg cgcgatgctg atcccggatt ccgtccccgg catttaccgg
     8041 gattttctcg gcgggatgaa agagtaaaaa agaccaaaag aggaacggcc tgctcaaaag
     8101 caggccgttc ctcttttgct gttatacttt gcgcgaatgc tttttcagca gacgggtcaa
     8161 ttcagccacg tcgatgggct ttgcgatatg cgcgttcatg ccgcattcca gacaacgctg
     8221 aatgtcctcg gcaaaggcgt ccgccgtcat ggcgatgatg gggaccgacg ccgaatccgc
     8281 gcggttcagc ccgcggatcg cctgtgcagc ctcatagccc gtcatgtgcg gcatccggag
     8341 atccatcagg ataaaatcat aataaccctc cggcgattcc cggaatttgt ccagacagat
     8401 ctgtccgtct tccgcccatt ccagctccac acccagatcg gacagcagtt cgtatgcgat
     8461 ctcccaattc agctcattgt cctctgcgag caggacacgg ctgccggaga ggccggcatc
     8521 gttgccggat gcgggctgat gcgtggcttc aaggcccata tactggcgca ggccataaaa
     8581 taaagtggat ttgaacagcg gcttggaaat aaaaccgttg atgccggcct cgcgcgcttc
     8641 cgcctcgaat tcggtccaat cgtaggcgga gatcagcaga atcgggattt cgctcccaaa
     8701 gctgcgccgt atctccctgg cgatctgaag gccgtccata tccggaag
//
LOCUS       DAWBSB010000084         8636 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1761_length_8636_cov_3.755273, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000084 DAWBSB010000000
VERSION     DAWBSB010000084.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 8636)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8636)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8636
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1761_length_8636_cov_3.755273"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1619 a         2641 c         2767 g         1609 t
ORIGIN      
        1 tgcacacgcc cctgtgccgg cgcacagggc tttgagaaca ttgaaccatg caaaatttca
       61 gcgagtgaaa ttttgtttga agccgccggc ggcttcaacg ccatctttct aagcggccaa
      121 gcgcgataag tggagcggcg ggctttagaa gggcggcgga acgtctgttt atccggactt
      181 tccccgcaac aggaggactg atcatgcgcg tactgaaaaa aatccttctc tggctgctgc
      241 tcgtggccgt actggccgcg ctcatcgtgg gcggggcggt ggcctggttt tactataata
      301 ccgaccccga agcattcgac gctcctgccg ttaccgcggg cggcacggcg ctggccgtca
      361 acggctatga atggaacgcc cctgtgatgg gcggcatcct gttcaaaagc gcgtccgcgc
      421 ccgccacgct ggaaacgcag gctctgggca cctgggagca ggcttcgctg gatataacac
      481 tgcccgcgga ccggcccacg catcttgtgg tgaaggacgc atccggcaat gtgctgctgg
      541 acgtaaataa cgaggctcag gtgaccgttg cgttccccgc caacggcaag tacacgctgg
      601 aagcggacgt aacggttccc cggcacagtt ccgccgataa acccggcgcg cccgcccacg
      661 gatacggcgt gttccggtac agcgcccgcg tcaacgtcag cgcacaaccc aaggtggaat
      721 tttccgccac gcgggtggag cagggcgcgg tgatcaccgt gctgctcacg ggctttctgg
      781 acgggccgga gccatccgtc gaaacagatc tcagcctggc ccaattctgc gacgtgcctg
      841 gcggaaaggc cgcgttcatc ggtgtgcact acaaccggga gcccggcgat tataccgtgc
      901 gcgtaacgtg cggcagcgtc gacgtgacgc agactgtgac tgtcgttcac cgggattttc
      961 ccaagcagaa catgaccatt gaccagagcg tggccgacgc cacggtgaac agcgccgccg
     1021 ccaatcagga atggaaagac gtaatgtttc cgctgtacgc ctcggcggac gcggagatct
     1081 actggagcgg cacgttcatc cgcccctgcg gcaccaccgc ccagaacacc cagtacggcc
     1141 tgtaccgcta caccaacggt tcgccttacc ctgaacgaca cgcaggcatc gattatgact
     1201 gcgacctggg cgacgcggtc tgggctccgg gccggggcaa ggtggtctat gccggctttt
     1261 tgcaaatgac gggcaacacc gttgtcatcg aacacggcgg cggcctcaaa agctatttct
     1321 tccacctgga ttcccttgcc tgcaagcagg gggacatcgt ggaaaaagaa cagcttatcg
     1381 ggcaggcggg cagcacgggc tattccacag gcccgcacct gcactacgag gcgcgcatcg
     1441 gcaaccagag cattgacccc gaccagcttt tcaacggcag cagcgggctg tactttgccc
     1501 gataaaccgc cggaacaagg agataccgga tggaaccgaa cgaacccctg aacgcggccc
     1561 cgcagccgga cgccgcgcct gttacaacac agtctcccgc cgcaacacag gagcgcccgg
     1621 ccgaaaagcc ccgcgctgcc gtccgcgtga cgcagcaatt tgtggtgcgc atgcagcggg
     1681 gccagaacga ggccgcgctg ccccccaagg gcggtaaacc gcccctgcgt gccacctaca
     1741 atgcagtggg ccagctgctc taccgcgtgg gcacacaggc ggaatacacc tgcatccggc
     1801 tggggcgcgg catacgcggc gcgttccggc tggcgctgcg cgccgcggcc gcgctgctgc
     1861 gctttgtggg cggcccgctg ctggcgctgt tttccagcat ctgggacgac cttacggccc
     1921 cggcacgccg ttttgccagc ggcgtgcgcc atatgcgcga cgccgcgcgg gcggaagccc
     1981 aggcgggcgg cagcggcaag cgggccggtt ttgcctattt taaaaacggc gtccatgcct
     2041 accggcatct gatcggcggc gctctgcgct acctgatgcc cgccgccgcg ctgtgtgtgc
     2101 tggttttcac cgtgcaggag gtgctgggct ccagcttctc gctgggcgtg agctacagcg
     2161 gcgactttct tgtattcatc gaaaatgaag gcgtttggga ttccgccgaa aaaatggtgc
     2221 aggaacgcgt gatggcgtcc gagggcgcca aggtggaatg ggacgagcat ccggtgttcg
     2281 agcttcgcat cgtggacccc gccgcccgca ccagcgccag cgacctggtg gacaagatca
     2341 tcgcggcatc ctcagacaag atcatgaaag ccgtgggcat ccgcgtgaac ggggagctta
     2401 tcggtgttgt ggaggacgga cgtgcggtgc agcagctttt ggacgacaca ctggccgctt
     2461 ataacgacgg cacccacgag cgcgtggaat ttttgtaccc catcgagcag gtgcccggca
     2521 cctatttcac aaactctgtg caggatacca cgatggtgct gcaggctttg cagtccagcg
     2581 acgtgctgag cgtaaaggtg acggacctca ttgaatatga cgagacactg gaatatacca
     2641 cagaggaagt ggaatccgac cagtacaaca aaggcgtgcg ctttatctcc cagcgcgggc
     2701 gggacggcac ccagcatgtg gtggccgagc agaccagcgt caacggcagc gtcatctcca
     2761 ccgttcccgt ggaagtgacc gtcaccgagg aaatgacgcc gcgcatctat accatcggca
     2821 cgcgtgaggc acagtacggt gtttccacag ccgccggcgg aagcgtcgtg ggcacgggct
     2881 ccatgacgtt cccggtgccc agcctctcct atatcaccac gcgctttggc agcggccacc
     2941 gcggcatcga cctgtgtgcg cccaacggca cgcctatcct tgcggcggat tccggcacgg
     3001 tcatcgaggc cggctaccac tggagctttg gcaattatct gcttattgac catggcaacg
     3061 gcatcaccac gcgctacgct cactgctcgg cgctgctggt gggcgcgggc tccaccgtta
     3121 cgcgcggcca gcagatcgcc accatcggca tttccggcgt cgccacaggc taccattgcc
     3181 attttgaggt agcagtgaac ggaagcctgg tgaaccccgc gccttatctc ggcgtaagtt
     3241 gaacctccgc ctttccgttt gcggaaagcg cctcgccctt ggccgcggat gaactccgga
     3301 cgcggccaag ggctgtttgt atggaatttg aaaagggctc gatgctgcgg gccgaagccg
     3361 gggatgagcg taaagaccgg cgcaaagaaa agcatgctga cggcaaggca gacgcattgc
     3421 ccgaatgcgc ggcacttgca cacgacaagc agggcatagt ttgtcgaacc gtccgcctga
     3481 atagggaaag ggagactgta cggaagccgg cgcagcatcc gccacacaaa ccctacggtc
     3541 gagtacttgg ccaacatact cgggaaaaat accatcctgc tgttatccgt aggcatacat
     3601 gctccctccg cttttcctat tggaagcggg aaccatccgt ttccaggagg gttatagtga
     3661 aaaagggcgg aaaggcaacc atctgtattt ttcacggaac gtaaaaattg cggccaaaac
     3721 gcttgacaga ggcggaggaa tgcattatcc tataaaacat actaaaatga taggaaatgg
     3781 aggcgcaggg catgagccgg gataccattc agcagcggct gtgccgctgg cggaacagcc
     3841 gaatgtgcat gggaggatga ccgccgcaca cccgcccgcg ccgtgcggcg cgggcctgca
     3901 cagaaagaag gctgttacca tgtctgattt taaaggtttg aacgtacatt ttgaaacgct
     3961 ccagctccac gcggggcagg aaaacccgga tcccgccacg gatgtccgcg ccgtgccgat
     4021 ctaccagacc gcctcgtatg tgttccacag cagcgcccat gcagccgacc gcttcggcct
     4081 gcgggacgcg ggaaacatct acggccgcct gacgaacccc acgcaggatg tgttcgagca
     4141 gcgcgtagcg gcgctggagg gcggcgcagc cgcgcttgcg gtcgcttcgg gcgcagcggc
     4201 catcgcttat gtgttcgggg cgctggcgcc ggctggccgg catatcgttt cctccaagac
     4261 catttacggc ggcacataca atctgctggc tcatacgctg ccgctttcgg gcgtaacggc
     4321 cacatttgtg gatattggcg acccgtcggc cgtggaggcc gccatccggc cggaaacgcg
     4381 cctgctgttt gccgagactt tgggaaaccc caattccgac gtggcggatg tggaaacgct
     4441 ggccgccatc gcccaccgtc acggcatacc gctggtggtg gattccacct ttgccacgcc
     4501 ctacctgctg cgccccattg aatacggcgc ggatgtggtg gtgcattcgg caacaaaatt
     4561 tatcggcggg cacggcacgt cgctgggcgg cgtcattgtg gagggcggcg ggttcgattg
     4621 ggcggccgcg ggccggttcc cggccctcag cgcgcccaac cccagctacc acggcgtcag
     4681 cttcacgcag gctgcggggc ccgcggcgct ggtcacctat atccgcgcgg tgctgcttcg
     4741 ggatacgggc gcggccatct cgcctttcaa tgcttttctg ctgctgcagg ggctggagac
     4801 cctttccctg cgtgtggagc gccatgtgga aaacgcgctt cgtgttgtcg attttctggt
     4861 gaaccatccg aaggtggagc gggtaaacca tccttctttg ccggaaaatg gatatcgccc
     4921 gctgtatgaa aagtattttc cccgcggcgg cggctccatc tttacgtttg agatcaaagg
     4981 cggcgcggcc gaggcgcagg cgttcattga taaactgcgc atcttttcgc tgctggcgaa
     5041 tgtggcggac gtcaaaagcc tggccatcca cccggcaagc accacgcatt cccagatgac
     5101 gccggaggag ctggcccaaa gcggcatccg gccaaacacc atccgcctgt ccattggcac
     5161 ggaacacatc gatgacattc tggcggacct tgccgcggcg ctggaggctt gaatcggaag
     5221 ccgcgctatg ccgcggaggg cttcaaagaa atacgcaaag cggcggcccc gggccgccgc
     5281 ttttgcgttg cgggcacagc ccgcggaagg gagaacgcaa tggaacaggc aacgggcgcg
     5341 cggcgtgccg ctgtggctat gagcgggggt gtggacagct ctgttgcggc gctgctgctg
     5401 gcacaggcgg gctgggcctg tacgggtgtg acgctgcgcc tgtatgcggg tgcggatgcc
     5461 gcgccggacg gcgcgcgcac ctgctgctcg ctggaggatg tggaggacgc gcgcgccgtg
     5521 gcacgacggc tgggcatccg gcactacacc tttaatttca cagaagcttt tgaacgggat
     5581 gtgatcgggc gctttgtgca gggatatctg gcgggtgaaa cgcccaaccc ctgtatcgac
     5641 tgcaacaaac acatcaagtt ttccgcgctg ctgcgccggg ctcagctcat gggctgtacc
     5701 catgtggcca cgggacatta cgcacgcatt gtccggggcg ccaatgggcg gatgctgctg
     5761 cagacagggc tggacgcggg caaggaccag agctacatgc tctacggcat gacgcagcgg
     5821 gagctggcgc acacgctgtt cccgctgggc gcgctgacaa aagcggaggt gcgcgcgctg
     5881 gcactggaaa acggctttgg caacgcccgc aagcgggaca gccaggacat ctgctttgtg
     5941 ccggacggcg attatgccgc ctttgtggag cggcacacgg gctgcccctg tccgccgggg
     6001 gatatcctgg atgaagccgg acgcgtcgtg ggacgccaca ggggatatat ccgatacacc
     6061 gtgggccagc gcaggggctt gggcattgct gcgccgcagc cgttgtatgt gcgcggcgtg
     6121 tgcccggcgc gcaacaccgt tacagttgcc ggggccggtg cgctgtatac cgacaccgtg
     6181 gacgcgcggg agctgaactg gatcgcctgc gacgcactgg gcgaacgtcc ggtgcgcctg
     6241 ctggcaaagc tgcgctaccg ccagcccgcc cgccgggcca ctgcgtggca gacaggcccc
     6301 gacacactgc gcgtgcgctt tgacgagccg cagcgcgcgc cctcgccggg acaggcgctg
     6361 gtgctgtacg acggaggaac ggtggtgggc ggaggcgttc ttttttccac ggtctgaaac
     6421 aaaaaacgcg cggcgtaccg cactggcacg ccgcacgttt ttttgttata ctggtaaaca
     6481 acggcggggc ggagcggcgc cctgtgcggg aagaacaaaa caggccgcgc gcaaagcgcg
     6541 cgggggaggg ggaaacgcct tggcgtccgg cgagagggat ctgacgcgcg gcagcgtatg
     6601 gcggcaggtg ctggcctatg cgatgccgct ggtggccagc agcctgctgc aggcgctgta
     6661 cagcatggtg gacacggtca tcgcaggcca ctttgcgggc agcgcggcca tttcagccat
     6721 caacaattcg ggacagatat taaacatcct tactaagatc gccatcggca tcacaacagg
     6781 cggcaatatc atcatcggcc agtattacgg cgcaaaagag caagggcggc tgggcgaggc
     6841 gtcgggcacg ctgctctcgc tttcgctgct gcttggggcg gcgacggctg tgggcctgta
     6901 cgcgggcgca tatccgcttc tgggcctgtt gggcgccccg gccatggagc agtctgtggc
     6961 gtatttgcgc atctgtgcgg tgggcatggt gttcgtattc ggttacaacg cgctttccgc
     7021 cgtgctgcgc ggcgtgggcg attcccgcac gccgctgctc atcatcggcg tggctacggt
     7081 ggtgaacacc gtgctggacc tgtggttcgt ggcggggctt ggcatgggca ccgcgggcgc
     7141 cgcgcttgcc acaactgccg cgcaggccgt cagcttcggc atggcgctgg tgctggtggc
     7201 gcggcgcacc gggctgctgc agccgaagct gtcgtttttc cgtatgcacg gcgcgctgct
     7261 ccggctcatt cttcggctgg gcatccccag tgcactgcaa atgaccattg ccgggttcag
     7321 ctggctggtg gtaacatttc ttgtcaatca gtacggagtg aatgtttcgg cgggcaacgg
     7381 cgtggccatc aagatcaagg acatctgcca gctgttcatc tccgccatgg ccagcggtgc
     7441 gaccacgatg attgcccaga acctcggcgc atccaagttc gagcgtgcaa aggatgtgat
     7501 gtacactgcc atgaagatca catgcgccat ggctgcggcg atgattgttc tggtggagct
     7561 gtttgcgccg cagatggccg ccgtgtttac aaatgaagcc gccgtgcggg atgcggcggt
     7621 gctcaacctg cgcatcgaga tcctgggaca gatcttctac gcagtgttta tggtatacca
     7681 cgcgctggcc atcggtgcgg ggcattccac ctttgccatg ctgtccagct ttgccaactg
     7741 catcgtgttc cgggtggtgc tcagcctgct gttcaatcat ttgtggggca tctacgggct
     7801 gtacgcggcc tgtgccgtag cgccttccat ctcggtgccg ctgggctggc tgtacacccg
     7861 ctcgggcgtt tggcggcgca gtttggccaa aacagaaaag taacctcccg caccggccct
     7921 ttcaggccat gcgggtgcag aaaacggcgg gtgcttccgg taagggaagc gcccgccgcg
     7981 ctgttttaca ttgttttccc gggaaaactc aaccggtgat tttttcaaat acttccgcac
     8041 cctggccgca gatgggacat ttgtagttgg cgggaagggt ctcgccctca tagatatagc
     8101 cgcacaccgt gcagcgccat cccgtcacct tttcttcggg cggacggtag ctggaggcct
     8161 tgggcggtgt gaggcctttc tttaccgcgt ggtaataatc ataggtcatc tgcggaacat
     8221 cgtccagcac ggcccagtca tccacgcgcg ccacgaacag gatgtgtgtg ccgaggtcca
     8281 cctcgttcac tacggtgcag gcgaagcgcg ccgcacactg ctctgtcaca tagggcacgc
     8341 cgtttatatc ccgcgcccag gcaaagcccg cgaacttgtc cacatcccgg ctgcactgga
     8401 acccgaaccg gccgatcagc tccatcgagg cgctctcggc cagcacagtg gccgagaatt
     8461 tgccggactc cagaatggtc cgggtggttt gattctcctt attgatggtc accgccacct
     8521 gcaccgggga aacggtcacc tgccccagtg tgttgacgat gcagcccgcc gcttttccgc
     8581 cggcttcgga ggagacgatg tacagcccgt agctgagttt atcgaatgcg tgataa
//
LOCUS       DAWBSB010000085         8596 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1771_length_8596_cov_2.204894, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000085 DAWBSB010000000
VERSION     DAWBSB010000085.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 8596)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8596)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8596
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1771_length_8596_cov_2.204894"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    793..802
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7183..7192
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1406 a         2808 c         2354 g         2008 t
ORIGIN      
        1 ccacggcttt ctcggaatct tcagccatgc cctgcaccat gtttttgacc tgatccagcg
       61 tctgcttcac acggctgatg tcaaacttca ggccgaggac gacgccatca tccgaagctg
      121 ttgtcggcac acatatccct ccttgccatg gccgcgtcgt attcggcctc ataatctacc
      181 gggtccagcg ccacagcgcg cttgatgtcc gcataccgct cccgctcttt ggggtcggat
      241 atcctgcgca tatccacgct gcggtaatag atcgcattct tgatctggct ctcaggcggc
      301 aagctgcgga aaatggacat gaactgccac cagtgcatcc gcgctgtgaa aaggtcgatg
      361 cccgcgtata cgcggaaatc cccccaaatg cgcagcgcgt cttttttcca gtccaggagc
      421 cgttccggcg gggccggctc atccccatgg ggcggatcgc cgcaaaaata gaagtcgagc
      481 accccctgga agtgcccggc ttcattctgc ggtatttcac ggaatgtatt gagcaggatg
      541 acgtcgaatt gttcctccgg cgtcaggtcg gacgccagca ccgttcccac atacttcatc
      601 caccaggccc agtccgtcgg cacctttcgg cccaggatct ggtccggcag ctccagaatt
      661 gccggataca ttcgcaaccg cctccttcag ctccgggatc aggaactgct ccacgacctc
      721 ccggcgtcct cccatgacct cgccgtaaat gtacgcacag aggctgatgt gttcgttgat
      781 gttctccggg cgnnnnnnnn nncgccgaag atctcccggt attcttcaac gcccagcgta
      841 ccctcgatga aggcgcggca catggctacg gcgtccgaaa attttttcag cgcctcctgt
      901 gccatggtgt ccgtgctccg tccgtcctgc ccgccgggct tcagtttggc gtccatcgca
      961 cagaatgcct gcgcggcctg aagcacgcgg ggaaaatccc gcgtaacgcc ttccagcatc
     1021 cgtttgtccg agatatcgca cgggtatgcg cggccgcaaa tggtgatctc gtgctttttg
     1081 gcgaactcaa accccgcgct catttcgacg ccgcctttgc ctctgcggcc tgcggcgaaa
     1141 acgtgggcgt gccgtcctca ccgatggtaa cagttccctt ttggggagtg cccaggatct
     1201 tgaggccgaa gctgatgttt tcgcggtttt gggcgtcgcc ggacccatcg tccgtgatgg
     1261 acagcacgcc ctctccctga cggccgttgg gcttgccgct gcccaaattg tcgtagcagt
     1321 caatgaactt gactttccgg ttgtcgaggt cgtacaggcg gtccatgatg gcgtcctgcg
     1381 cagcgtcgcc aaacctgcgg aaaccggtga aggtacggga tacgctcacg cccgtgacct
     1441 cgctttcggc cacgccccgg cccgccatgt cgtagtattc ctggctttgt tcgttgatgc
     1501 tgttgccgcg ggatgtgatg ccgacggaaa cctccgccca ttggggcgaa tcgtttgcgc
     1561 tgaggtcgat ccaccagcgg cggttccatg tcatgggggt aatatatgtt ccaggcatgc
     1621 ttcgtcactc cttttcatag atcagggtgc cctgaatttt gtagacacca tattggaaat
     1681 tttcgtccca gtctgtaaag gctccgtttg aggcggaaat tgagacaaaa gaaaggccgt
     1741 tcccggaaag agggacgccc ttgcggtttt gctgctggac ccagtcctgt aactcttctg
     1801 taaagttgca gttatccagc cgcatgatgt catcagccgt catgcgggtg gcagcaatga
     1861 taaagttgta ttgccaggtg gccgctccgc gcatatcctg ttcgatgacg acagaaccgg
     1921 ccgggaagat cccgtacccc tcggcgtccg tctccagctg gtcggtgcgc gcctccagat
     1981 ctttcagcgc ggggcactgt gcgaacaggg cgcggatatc gtttaaaaca gccattatgg
     2041 gcctcctaaa atgttccggc gttcatcctc aatgatccgg tctttttcgg cagccatgag
     2101 gcgctcaagc cagaaaggac cggcaaggcg gttaaacgta gtggtgtacc ggatgtcttt
     2161 gtttgtaacg tgtttcggct tgcgcccggc catgactttc ccgaaataca gatacttgac
     2221 ctgcgggccg cggatgacga tgcggccgtt ggccggttcc tggccctggg cgatggcgtt
     2281 ctcaatgctg ctgtatgtgc gcttgggcac atacttggtg atgcgccgcg caacgttttt
     2341 cacaagatgc tgttgggcac gtccgccttc ttcaagcccc aggtccttta tgaggtttcc
     2401 aaactccggc aaatgaaagt cgagctccag catgctcatt ccgtcgtcac ctcacaatgg
     2461 ggcaggccgc cgaatgcaca gtgcacaagg tttttgacag tcaggggctt gtctgcgatc
     2521 aactcacgct tgctctgtgt gtccgtgacc tcgcccatac gctcgccttg caccacgtag
     2581 tccttttccc gtttttcgcc cttgtactgc tccggcagtt caccggggat gacgatgctc
     2641 acgccgtcct ctccgtcctg ccagaagcag gcggggacat acacccgcgt gtatgccccc
     2701 ggccccgtct gcaggtacaa cgtgcagctt gcattcggcg tcagcatccg ctcacccccc
     2761 ggtacagcag cccgctgcgg atggggatat attgggcggc gatacgcagg cagcggcgct
     2821 cgaagctgcc ggattgttcg tcatcctgct ggatgctgcg gctccatttt cccacgcttt
     2881 cgctggtcac tttgccgccc gccagcacgg ccgtgtcctg ggcgtgctgg gcgtctgcca
     2941 gcgcgcagtg cgccatgcgc acgcatttcg ccagcgtttc gtcctgctcc caggctccgt
     3001 cctgcaggcg gtccagcgtc agactgtgca gcatgtactc cgcacgggcc aggcagccct
     3061 ccacgtccgc ctccgaaagt tttccgtggt atgtggaata gtagaactcc ttgtccgcta
     3121 ccatgcgtat ccctcccgtg tattactccg cggccgtttc cggctgctca ccgggcacag
     3181 cctcagccgg tgcggcagaa ggttctaccg ccgtctgcac ggccagcagc aggtcctgct
     3241 tcttggccgc gcccgtcacg tcgatgccgt gcgccgcagc gaagctgcgc agctctgtca
     3301 ccgtcatatc ctccagcgcc ttttccggtg ccggggcagg ggcggggttt tgtgctcccg
     3361 ttcccggtgc ggccgtcccg gcctccggca gggaggcccc ggcctcgacg gccggggcgc
     3421 tctgcgtttc ctcatgaaaa gtcaaaccta ctgtccgcat attttacgcc cccgcttctg
     3481 ctgcgctgtg caggtagatg cccgccagct tgttggcgta cacgtccgcg atgcccacgg
     3541 tgcggtagcc gaacatgtgg cggtcgccgt ccaggtcgtc ctccggccct ttgattttgg
     3601 gcaccgtgtg cttctggaac tggatcagag ccgaagggtg gaacaccatg aagttgatgt
     3661 tcttcgcacc cgtggccttc ttgtaaccgc ccgcctcttc gccggaggtt tttccatcca
     3721 gcatgtcgat ggccgtatag aaccgggtct gcggcaccag cgtctttgtg gcaaagcgcg
     3781 tcaggatctc acggctcttc gtggtgtcca ggtctgcgat catgccgtcc agcgtcggcg
     3841 tgatgaacag gtggcggttt tccgtgggca cctcatcttc gtccatcttc gtgatggctg
     3901 cacgcagcgc cgccagcacc gcagcgccgt ccggcagatc ggctgcggtg gccttggaaa
     3961 tgccgctctt gcccgcgtag gatgcgaagc ggaaagcgtc cagctccggc accaccttcg
     4021 tgcggatgaa ctcgccggcc aggcgggaaa aggccacttt cgcggtctcc tgttcgtcca
     4081 gtgcgtccac atagaactta cggccgcggt cgtagttgca ttttatggtc tcgttggtca
     4141 tggtgacgtc gcccttgacg tacccgtcat tgcggctgta gtcgcccagc ccctgcatgc
     4201 tcagcatggg cacgatcagc tcattggcat tcgcgccctg ccgcgccagc tccgcagcgc
     4261 cgtccagctt gctggtcagc gacgccagct tgtagatttc gtccaggatg gggacgaagg
     4321 tttttgccag tgcaatgttg tttgccatat tggattacct tacctttctt tgtgtggtgt
     4381 gtgatacggt cagtccacgg gcagatccgc agccttgcgc atggcgatct catcggcggt
     4441 atatttcgcc gtgcccggca cagcagaggt cccggtgccg gatgcgggcg cgggttccgc
     4501 tgccgctgtg ccgaacaggt atgagtcgct tttctgcacg gccgcgatgg ctgccgcgat
     4561 gtccgcatcc tggttcttgc tggtgcgcag agcgtcaagg tccagcgcgc cgcgcgccag
     4621 cttcgggtta tgcgctttcg cgtcggacag ggccttgtcg atcttcgcat cgaatgcagc
     4681 ggccgccagt ttttcgtccc ggtccttttc cgccgcctcc gcgcgctgct tgtagtccgc
     4741 gatttgcgcc tttacctgct ccgcgtccag cccttcaaac ttaccgatgg cctcgttggc
     4801 agcgttcagc tgttcctgca ggccgtcggc acgcgccgat gctgcgtcca ggtccgcctt
     4861 tggcgcgtac agctcgttga tcttcgcctc cagcttttct tccagctccc cggtgtaggc
     4921 gtcgccaaga agctctttgg caaattccag tgccatgttg tcagtctcct tccttttaaa
     4981 tacaatatat atgtgcataa caaaaggacc cccgaaaagg ggtccttcag ctatcttttt
     5041 atgggcattt tatgcccgtt ttcacgccgt ttaaagcggc tttttaagtg tcttttcaaa
     5101 ctcttccaaa atctctttct ggatcacgct gtacggccgg ggatctgtgt actgcttgat
     5161 cagggcgtcg atatcttcac cgggaaattg cgcgcgcaac tcttccatgt aggcgaggcc
     5221 gccttccttg tgtacctcca tgcggtacgg cggatacttc tttccgtatt tatcctcata
     5281 cgcccgaagc agctggtcat agcccaattc ttccatgcgt tcagcaatgc ccggtacaaa
     5341 aggcaaagac atcagaaatc cctcctaaaa tagcttctgg tattcatccc acaactccgg
     5401 gaacaacgtt ttaacaaagt acaggtccga atcgccttgc gtttccatag taaacaattc
     5461 agcaaaaatc tccgtgaatg taaacgacgg gttcctttcc cagtattcga ccgaatggcc
     5521 aaatgctgta tatagttttc ccgcgctcaa tgcgctgaac acatccttca acggtttact
     5581 gcgtatattt tgcgccaccc gttgatagcc ctccatgttt ttcaaaattt tttcgcccgc
     5641 aagctgtgcg gcacgcttaa agccttcatt tctgtatgac ctcatatgca ttacgtccgc
     5701 gcggtgtgcg ttttcatggg tgttggcaaa atcgaaatcc atttccgcaa agccgggggc
     5761 attcgggttg tacaaaattt tatctttttt tgccgaatat gcaaacgggg cagccagtgt
     5821 gggatcttct tcgtatccag tcggactttt ttccgtaaaa tgtcttaacc ataccatatt
     5881 cttttccggc gcatccgtca aggtgtcaag atacgcggaa tagcttttcc gggcttcttc
     5941 cacatccagc ttgcctccgc tgcctgtgag cgtacccgca ggacgcggca tttccagcgt
     6001 gccgtccggt ttcagcgccc ggcccagccc cgccgcccgc agccgctccg gcgcggtgcg
     6061 cagcccggcc gcagccgaaa accgcttgta ttccgcattc agctgccgca ggaggatgcg
     6121 gctgctgcgc agttctccgc tgcccagctt gccctcctcc tgcgccgcgg caatacggtc
     6181 cttgcactgc cggatgctgt tctccagcgc cttctgctgc tgtgtggctt cgtactgggt
     6241 gtagtggcgg ccctcgtagg tgacgccctt cgcgttttcc cgtgctatct ccgccagctg
     6301 ttcctccgtc cactggggac tgtccacgcc cagcttgatg ggccaggcaa tgtgcttgca
     6361 gctcagcgtg ccgatgcgcc gctgcagccg gctgttcagc cgcctgtatt ccttatcgct
     6421 gtactgccgc ccctggatgg gttcatggtc cggcgcgcac gcgctgtgcg cgctgatctc
     6481 ccagccgtcg cacccgccgt cgtcgtggtg catctcgttg atggccgtgg tcatttcgcc
     6541 catcttcgcc ataatggcgc gctgggccat gaactccacg gaaaaagtgc gcccatccga
     6601 gcgctcgatg gtgcggatgc cccgctgcca cagccgcagc gtcgcccgcc gcactgcttc
     6661 gtccggtgtc tttgcgccgc tggagacctc ccggaacaca tagtccatcg tgcgccggta
     6721 tacatcttta ataggataca cccggccgtc cacatctgcc gcggccagct ggcccagcac
     6781 gttggccact tccttgcgcg ttaccttgac gtaggcttct gcaatgttcc gcaggctttc
     6841 attttcctcc agcggcgcgg tcttctcggc ggcccagctc atgagctgcg ccaccgcgtc
     6901 gtcggtgagg tccgtctgtc tgcgcagcgt gtccgcgatc acatcatccg cgcccgcaag
     6961 gctttttgcc agcagaagct tgtattcatc gccggacgat atctgcccgg cggcggtaat
     7021 gcaccggcac aaatcgcgca gcagctcttc cgtgacgggg ccgtaaacag cgagaatcag
     7081 ctcccgcagc ccgtcgatct catccggcgt cagtgccata acgtcacctc agcccggccg
     7141 gggcggtcag ctggaccatc cccggcatgt acttttcacg gannnnnnnn nnctgccagt
     7201 ccttgtccgc gtcgtacaaa acgccgttgc cccagctcac gctcagcagc tgctccaggt
     7261 ccacagcctg ggcgtcgcac agccccagcg cctgccccca caggtccgtg atgcgcagcg
     7321 tctccatcag tgcgtcgtac cacatccgct gcaggtccat gatcgacagg ctgtagtcgc
     7381 cctcgctgct gctgatctcc tttgccgtgc gctccacagc ctccacgtcc gacaatatac
     7441 cgcgtttcag gccgatgata ttttcacacg cctttaaata gctctgcttc cgccgttcaa
     7501 agctctcatc ccgcagcgca ggggagaaaa tggtcaggcc tttgtcgttg gtgtcgccgt
     7561 ccagccccac gaacacgtcg tccttcagcc gcatcacgcc gccctccggg cccggcgtca
     7621 taagcatatc cgagcccgcc acgatccggc tgcgtcccag ctcgaactcg cgccccagct
     7681 ggtactcatt tttgtagatg ttgtggatca gctgcaccgc gccctcgtac acgctcacgc
     7741 cgtccgggct cccgtccacg ttatttgcca tcggcatgcg gatgtaggtc atgcccaacc
     7801 cgccgaaggg cacgctgtag gtgtgctccg gggccagcgc cgcatactgc ggcaggctgt
     7861 ccagccgcac ctcatgcccc agtgtgctgc tgttttccga cacatacagc ttgtaccgga
     7921 tggtcagata cccgctgcca tccacagtcc ggcgttccag cagcgtgtag tagtcggagc
     7981 ccgcccggct ccgctcgctc atcagcacgt ccgtgatgcc gcggggcccg cgggccagta
     8041 cgttgtagca gtcgcgcctt accacatggt aggccagccg ccccgtgccg tccggtgcag
     8101 gcttcaaaaa gccttcgccg cccaccatga cccactgcag cacgtcctgc ttttcggcgt
     8161 cgatgaggct gcgctgcccg tccagccacg ccgtttttcc gatgccgttt tccgtaaagc
     8221 tggaatcgta ctccgcaaaa caagcctttg tcagtttgtt ggtgatggtg taggggatgc
     8281 gctgcgccgg gtcttcgtcc ttgcccttca ccgcttcacg catgaagaac agctcgaacc
     8341 actcccgcac cgccgcccgc atagccgctg tgctggtgtc cttcagcccc gccgcctgcg
     8401 cccctgtgat ggcggcgtcg tcaaacagcg cccttacgac tgcgttcatc cgccgtcact
     8461 cctttcgatc cgaatttccg gctgctttgc ccgcagcccg cgctccacgc cgttgatgta
     8521 tgcccgcagc gtgcggtttt ccgcctccag ctcccgcaca cgcttctgcg ccgccgccag
     8581 cgcgtcgccg tattcc
//
LOCUS       DAWBSB010000086         8583 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1773_length_8583_cov_2.631332, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000086 DAWBSB010000000
VERSION     DAWBSB010000086.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 8583)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8583)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8583
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1773_length_8583_cov_2.631332"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1720..1729
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4040..4049
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6471..6480
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1736 a         2688 c         2349 g         1780 t
ORIGIN      
        1 gaaagatatc gtgctacgct ggacggaaga gatcgcgccg ctggcacagt gaggccgcgc
       61 atatggcaaa ccggaaaaaa cacagatggc tgccgtacct gatggttctg cccgcgtttt
      121 ccattatggt tctggtggtg gtctttccca tcgtaaacac agtggcccgt tccttccgaa
      181 caacggacag gcagttcacg ctggacaact atgtctattt ctttaccagc gccgaggcgc
      241 tgcaaagcct gctgttcacc ctcgccgagg ctctcaccgt catggcactg agcgtagtgc
      301 tcagctttct gctggcgctg tacctccgct tttccaaaag ccccgtcagc cgcgcgctgg
      361 gcaaactgta tctgctgccc cgcttcatcc cgggcatcgc cgcagtgtac gcggtgatga
      421 atgtcatcaa ggactccggc agcatcaacc gcatcgccgc agccttcggc gtcgattaca
      481 agcccggcct gctgtatgac ctaaaaggca tcattctttc aaacctgtgg ttctgcattc
      541 cctttgccgc catgatgatg agcgccgcgc tgtccagcgt caacgattcc tatgtggaaa
      601 gcgcgcgcga cgccggatgc tcctggcggg atgttctgtg ccgcatcatt cttccgctga
      661 catacaagga cgtcatcgtc agcgccacct ttatccttat gggccaggtg ggcgcgttca
      721 cgattcccta cctgacaggc cccaacaatc caaaaatgct gggcatcctg ctgtaccaac
      781 aaacgggcac ctacatgaat tatgaacgtg cggccgcact ttcggtgctg atgttcctca
      841 tctgcctggg cggagccatt gtatacatcc gttcgaacat gaaagaggaa gtgtgggaaa
      901 caggcaaata atttgcccca cggaacatgc aaagggcggc ccggcatacc gccggaccgc
      961 ccttgccgtt cctcatcaac aaaaaggagt tttcgatcat ggagcagacg atttgtgtca
     1021 acgcccctct ggcactggcc ggtacgcgca acacccgcga attgggcggc taccccgccg
     1081 cctgcggcag gaagacgcgc aggcacgcct tcctgcgttc cgacgggctt tccggactga
     1141 cggacaaaga cgtgcagatg ctgctggatt acggcgtatg ctgtgtggtg gacctgcgca
     1201 gcgaaagcga gacggccgcc gctccctccc gtcttgcaga cgtacccggc gtggattatt
     1261 attccatccc catgctggac gaggcggcga gccagggctt tacgggcgga atgcctgagc
     1321 gcatgggcga tgtatacgtg aagctgctgt ccggccgcgg cgacgcattt gcacggataa
     1381 tccgcatttt tgcacagcac agcggcggga cggtgctgtt caactgtacc gccggaaaag
     1441 accgcacggg cgtgacggcc atgctgcttt tgctgcttgc gggcgtgccc tgcgagattg
     1501 tcgttgccga ttacagtgta agcgaagcca acatgcagga gatcttcaaa cagcagaagg
     1561 cctggctgga aaaaactttc ggcgtcaccc cgccggatgc ggtattctcc tctgcgccgg
     1621 aggagcttga aacggcgctg gcctatctcg cggagcacta cggtaccgcg gaatcgtatc
     1681 tgctgcaggc cggtgccgcg gcgcaggata ttgctgtcgn nnnnnnnnnc ggcgcccggt
     1741 cttttgtttt ctgttttttc gcgcaaagct actccagccg cagcccgccg cgcagcagat
     1801 gctccagcag cgccccgcat ccctcgcaca catcggcata tgttgcgtca aaatctccgg
     1861 tataccaggg gtcggcaata tcacgggggt gagcggagta atccagcaga cggtacattc
     1921 tgcgttccgg gtcgccgcca aaaatgcgcc gcatgttgga caggttgtac gaatccatgc
     1981 ccagcagcag gtcccaggcc ctgtaatcgt ccgggcgcag acgtaccgcg cgcttatccg
     2041 cgcaggaaat gcccacagcc tgcagctttt cccgtgtgcc ctggtgcaca gggttgccca
     2101 gctcttcacc actggtagcg gcggaagccg cgtggaacag agcccccatg ccacgttttt
     2161 cagccatctc ccgaaacaca aattccgcca tgggcgaacg gcagatgttt ccgtggcaga
     2221 cgaacagtat tctggtcatt gcttccacat tcctttaccg ctctactttg cgctgcgggc
     2281 ggtccgcttc cagcagcttt tcttccccaa agacatccag tggggagaaa agctccatgc
     2341 gcatgccgtc cccgctggag gcaaaacgca tttcaaaggc atggggcgta ttcaggctgc
     2401 ggcactccag caccagcagg tctccgcgca tatgcgccgc ggcggcgaaa cgcagcggcg
     2461 cgcgttcctc cctcggcatc tggtggaaga tggcgggacg gaaatttttc gcccagagcg
     2521 tattctcccg ccacgtaccg gacggcgcgc acaccttctg cacactgctg ccgtcgcaca
     2581 gccatacaca ggcggcttcc ccgtccacgc gcacacgcag gcctgtgaaa cccgccgggt
     2641 ttggccggaa atccagccag cccgtatccc attctctgcc gtccagcctg ccttggggta
     2701 tgtacgcctc cggcagccct ccgtctgcgc acgcgccgtc ctgcttatgc agattttcca
     2761 gcaggagcca cagcgcgtcc agctcggcac tcatatttgt gcactcggcc gtaacggcgg
     2821 ccgcaattcc gttttcaggc agaaccacac acaactgccc aaaggcgccg tcgccgcgga
     2881 agcctccacg ccggttcatc cagaactgat agccgtagcc cgcacaccag tccggcgtgc
     2941 cgtttcccgc atttacggag tgcggctgcg cagccatatg tacccagtcc cgcggcagca
     3001 cccggcgtcc cgcccactcg ccctcgttca gatacagaag ggccagcttc gcgacgtcat
     3061 cgcaggatgc ctgcagcccg gtgccgccct ggcaccgtcc gtctgcacag gtcagccacg
     3121 aaaactcctc cacacccagc gcggggaaca ccgtgcgtgc cagaagctcg ggaaccgtgc
     3181 ggcccgcggc acggcgcacc agctctgccg cgagatagga ggcggcggta ttataggcga
     3241 aaaacgtccc cggctcatgg gacagcggcg cttcaaaaaa agcgcgcacg ccatcctgcg
     3301 aaaatgccgc ctgcggcatc acacaggccg catgccctgt attcatggag agtacatgcc
     3361 ccacgcgcag gcggctccaa cgcgcgtcgc ccgaacacag ggatgcatat tcggggaacc
     3421 agtccagcac ccggtcgtcc ggatgcagct ttcccattcc aaacgcaact cctgcagccg
     3481 tggaagtaaa gcttttgctc agcgagtaca tttcccgttt atcgccgcag ctgtaaggcg
     3541 cgacggccca gcggaccata cactcatttt tttcaaaaat ctccagcgcg tgcagctgca
     3601 ctccaacggc gcgggcgttc cgcccaaact ccgcaagggc cgcatgcgaa acagacgcat
     3661 gcacaaaatt ttccatatcc catctctccc cggccgcgcg ccgcgcggtc tgtttttttg
     3721 caaagccgcc gcgcccgtcc gggcgcggtt cagctttcag agttcttgat tgcccgatag
     3781 gtgcgcggcg aaatacctac gatctttttg aatgtgcgcg aaaagtgcgt cgaatcgttg
     3841 aagcccacct cgtatcccac ccaggtaatg gaattggccg ggtccttcaa aagctcaatg
     3901 gccttttgga tgcgcaggtt cataatatac tccaccaagg tctgctgggt gagcagcttg
     3961 aacagatagc agaaatatgt tttggacacc atggagatac ggcacacatc ctccagcttg
     4021 agcggctcgt tgtaatgcgn nnnnnnnnnt aatgcgcttc cacataccgg atggcatctt
     4081 ccaccatagg cttgtatttg tcgtaaacgg ccgctgcggt atggctttgg ggcgactgag
     4141 aatattcccg tgccagtaca agcagcagct ccatgatctg cacgcgcagg aattcctcat
     4201 aatagatatc tccgttttcg tattccacca gcatcgcgtt catcagcgtg ttcacgcgct
     4261 ctcccgtttc aggccgaagc gaaaactggg aatggatgtc atcggcatcc ttgaggaaca
     4321 gccaggcaaa agaaagattc agcgccgctt catgcacctg ctgataggcg ctgttctcct
     4381 tcggcggaaa aaaatggtcg aaagaaaatt cgcagctgat gatctcggca tcttccagcc
     4441 gcaccacctg atgctcgacc ttcggaggaa cgatgaagat atcgccgcgg gccatcagat
     4501 gcttttgccc ctccacccaa tgctcacagc agcctcgggt gatataccaa atctgcatat
     4561 aatcgtggga atggggcaga tagccggagg gattctccga tgtgacctta tagatcttac
     4621 agaatctgct cttggtgaac atcgtgctct tttcaaataa aacggccggc tcattccata
     4681 cttccatcgc tttgtccctc cttctgatat accggcgcag ccgtgctgct gaaaaacggc
     4741 cgcgcgcccg ccatgtgcgg acgcgcggcc ccgttgccgt tctgcgtggt tcaatgtttc
     4801 caaagctgcg cgcatgcgca tcggtttatg cccggcacac agccgcacaa gctgctggct
     4861 cgaaaccatt atagcacacc gccgctggaa tgcatccggc ttttaccaaa atgtcacatt
     4921 ttttatgcgc gtgtctcccg gtgctttttc caaaccagca cagccagcag gaccacacaa
     4981 gccgccgcgg ctacggtgac ccagaccagc gggaacgcat cccctgtccg gggcattccc
     5041 ggcgcaggtg cggccgtacc ggaagacgcg ccccccgcgc cgccggaagg cgtgccggaa
     5101 gaggaagatg tgtctgcaga agaactgctc gtcccgcccg aaggagcgga agaagaggag
     5161 ccgccgcttt cggcgcctgc ctttggctgt gccaatacat acaggctcag atgtgtcagc
     5221 tccgcattca gcaggccgtc tgctgttttt gccgtcagca gttctttttc gccctcaggg
     5281 cttacgtgat acagctgcgt tttggctgcg tcaaagccct ggggaagtgg gaacgccaca
     5341 ttcagcacgc cgcgcggctg caccgtgccg ccatccagca gcaaagaaat atcaaatgcc
     5401 gcaaggtcaa cattttcatt gcggaaagcg cgcgccacag aagcatatgc cgcgtcgccc
     5461 gcatccaccg gctccacctt gagatgcgcg ccggccggca ctgtgtccag ccctgccgca
     5521 acacggatcc ctgttgcact gtccacctcg ttgaacacgg gagcgggctg ttccgaagtg
     5581 agcgcgccct ccacggtgaa ttcatcaaaa gccacccagc cgaatttggt atttttgtcc
     5641 gtgttgaagc cgcgctcaaa ctcggccttc aggtatttca cacgcaccgg agcaccagtc
     5701 tccagtacaa agtggtaggc gtccataccc gtggcgatgg cttcatagcg gcgagcgtcc
     5761 gcctgcgcca cggtgccgag ctccgtccag ctcttgccgt ccagcgacgc ggaatatgtc
     5821 accttatccg gcgcgccgat gcccgcggat acccaggcaa gatagtctac ccccaaagcc
     5881 tgcacctcag cgacagtgtc aaaggagatc tgcagcgtaa aaggctccgt agccacggtc
     5941 tgcaggacgc cgatataatc accgttcagc gtattctggt tcgcccagct tcctgtttga
     6001 aggacgccat tgttcagcag tacagaagcc acattctgaa gggttgcaaa gctttcactg
     6061 ttcaggacat ttccgctttc caacgttatg gtacccatag atgcgatgcc ctcgcccgaa
     6121 gccagcggcg ccgtgccgtt taccgtgaat tcgtccacgc ccagccatgt atagggcgca
     6181 gccgggtctt tggaggcgtc caggccgtgg ccgaactccg cgcggatata acgggccttt
     6241 tcggcggcat ccagcttgag cacatactcg accgtctcga agccgttggc cgatcccggg
     6301 cttatctccg cctgcgcata ggttacagta cccagctctt tccatgtctg gccgtcctgt
     6361 gaaacactgt atgaaacact gtccggcgcg ccgatgcccc atcccggatt ggaaacggag
     6421 cccagcgcca cacgctccac ctgcccgata ccgcccagat ccgctgtcag nnnnnnnnnn
     6481 aaacggcccc tgcgcgtgat tggcattgat cttcacatag ctgtcgttcc aggcgccggc
     6541 cttttcattg ccgtccacca gtttcggaag cgcctgcagc agcttgttga attcttcggt
     6601 attttttaca ttgtccgctt ccagtgtaag cgtgcccgcc gccgcaagat tcttgacagg
     6661 cttctccatc tccgtgggga tgccgctgaa gaaatcatgc atcgcgtcgt agatataccg
     6721 cgtgcccgag acgttctggc tcgtctctgc aatgccccat gtgcccgtgt accaggcaat
     6781 caggctgttg ttctgatagg caagcttgtc gtccgcccct tgcgtgaggt aagagcggaa
     6841 ctggctgatg tatttgtcgt tgatgccgcc gaattccatc tcgatgccca ggccgtactt
     6901 tttgcacagc gcagcggtat ccgcaatgcg ctgttcggag gcattggcgt cgaacgcata
     6961 gttgggctgc atgatggcgt agtcaaagcc gaaggtcttc caggcgcgga acccggagga
     7021 ctggtagaag gggatccagt aaatttgata cgcgccgttc tccaggctgt ggacgtaatc
     7081 cgttacaccc tgcaccgttt ccagaaccaa cgggtccacg tcgtagtgca tcacttcatc
     7141 gtaccaatag aagccgtcca gacgcaggtt ctcgtaaccc ttggccttga attcccgaat
     7201 cacggtgtcc agataccacc tggaggcctc catgcggtcc gccagagcct gctgcgccgg
     7261 attggtatcc gagaccgcga aattcagcga atgggtgccg ccgggcagcg tgccgaaggc
     7321 atcctggcac agaatgggcg gataaaacgc aatcttcacg cccactttgt aatccgcgtc
     7381 gcctgtggcc tctttcacca gggccacagc ctcgttcagc gcgtccaggt tataagccgt
     7441 gcctgtaccc tcgccgtagt ggaacagaaa atcaagatag gtctgccagt cctccgcggt
     7501 ggcacacatc tgtgccgcat cggggttgga ggtcatcgta ctgctcacat agatgaggtt
     7561 atggtaatcc gtatcgtcat ccggcgtatc cagatttggt gtatagccgt gggacaggaa
     7621 ggtaacggat tcaaaaaact tatccgtaat attgccctcc ccgtccacat agcctaccat
     7681 gggaagaagc tcctccacgg ttttctggct ggtctgcagc gcaccgcccg cggaagagaa
     7741 ccagtgtccg gcataggcca gcgcctcgtg ctggatgccg cgtgcctggt ctgtccaggc
     7801 atatttgttc acaacttcat aatccgtgcc cggagtatcc ggcagcgttt cggcggtgtc
     7861 cgaaggcgcc gctgtgcccc gtacctccag ctcgtcggca aaggcccagg tgcctgtcag
     7921 ctcaaacgcg acctttacat aacgcgcatt gtagttcagg ccgtccagcg taaaatagta
     7981 gctctggcaa ttcacctctt tgccgttttc caccacggaa taataatggt ccgggtcctc
     8041 gtccttcacg gcgtcgaagc gggaaacacg gcccacgcgg tggaacgtta cgccgtcctc
     8101 cgacaggaag taggtgacca tcgacggcgc agaaatcccc catcccaggc cctcctgcat
     8161 gtggaaggaa agcgattcaa tatggctcag ctgccccaga tccagcgtga tggcgcggtc
     8221 gatctgccgg tatgcctcat agtaatggga gcgcatgccg ctgccattcc aattgccgtc
     8281 caatatctcg ccgctgccgt aatagccgtc tgtcaggcgg ttgaggatgc tctcctcttt
     8341 ttcgcggatc gttgtatcct tggtggcagg gtcaaggtcc gtaccggcgc cttccatttc
     8401 cagtgtgaag gcatcattgc cctttccctg cgccaggttt acagtatctg ccgccgttgc
     8461 ggcgctggct gtatccgcag aaccggaaga agatgagaca tccgaagaca ccgtctcgga
     8521 ggaagacgaa gcgccggaag aggacgccgc cccggaagag gacgaaacgt ccggagagga
     8581 cgc
//
LOCUS       DAWBSB010000087         8453 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1791_length_8453_cov_4.094070, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000087 DAWBSB010000000
VERSION     DAWBSB010000087.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 8453)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8453)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8453
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1791_length_8453_cov_4.094070"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1607 a         2532 c         2727 g         1587 t
ORIGIN      
        1 cccttcaggt cgcttaatta catattttct ctaacttttg gggggcactt caaaagcggc
       61 cgcgcctgtt ttggttttgg cggaagacct actggccgaa caggcccgcc aggccgccgg
      121 agacgccttc gccccagctt tttacagcgt ccgaagcgct ttgcagagca tccactgcgc
      181 cgttcaattt ggcgacggcg tcgttgaacg cctgtacgtc catctggctg atcttgccgt
      241 ccagtgtgct gatggcgcgg tttacggcgt ccatatcgag cttcgagacc tgctcggcca
      301 cagcggccag atccacgcct tccagcgccg cggagatgcg gcccatgctg ttcatcgtat
      361 tgcccaggca caccaccacg gctgccagca gcacggccag tagcgcggcg gcggcgcgca
      421 tccaaagcag ctggccgcgc agcagctcat tggtgcggcg tgtctcttcc agaatttcat
      481 tttgttccat ggtcaggctc ctttcctatg cctttgctgt gatgatagca cgaaaccgcg
      541 ccgcagtcca tcgtttttgc gcgaaacagg ggcggctgtc cgccttgtgg aaaacggcgg
      601 gatgtgctac catgaaacaa cagacaggag ggatgatccc atgacggagc aggagatcga
      661 acgcgatatg tatgcgcgcg ctgttgcgct gattgaagaa cgatatccca cgggttgggg
      721 cggcgcggcc gtgctgcgcg ccgaggatgg acgctgcttt accagcgtgg cgctggaaaa
      781 cgccaactcc agcgtgctgc tgtgtattga ggtaggggcc atgtgtgagg cgcacaaata
      841 caatgtgaaa gccacacact gcctgtgtgt ggtgcgggat gacgagcacg gcccgttcaa
      901 ggtcctttcc ccctgcggcg tgtgccagga acgtctgcgc tactggggtg cggaagtaaa
      961 ggtgggcgtt accacaccgg atggcgcact gcggttcgta ccgttgagag aactgcagcc
     1021 gtaccattgg accgctgcct atccggagga ggaactggag cgtttcggca ggtgagcgga
     1081 gactgtcggg cgcgcgggtc gcagagaaag gaacgtcgtg atgggaaaca aaggcgcagc
     1141 caaccgctgc aaatgggtca atctgaaaaa cccggcgtac atccggtatc atgatacgga
     1201 gtggggcgtg ccccagcaca aggatgaagt gctgtttgaa atgctgtttt tggaatggtt
     1261 tcaggcgggg ctttcctggg agtgcatcct gaataagcgg gagggcttcc ggcgcgcctt
     1321 cgacggcttc gacgcggaaa aaatcagccg atacgaagac ggagagctcg cccggctgct
     1381 ggccgacccc ggcattgtcc gcaacaggct gaaaatacgg gccagcatcg gaaacagccg
     1441 tgtgtttctg aaaattcagc aggaatacgg cacctttgac gcatatatct gggggtttac
     1501 agcgggaaag acgatcgtgg aacgctacga cgtgcgcacg gcctcgcctt tgtctgaccg
     1561 gatcgcggcg gacctgaaaa agcgggggat gaagttcgtg ggttccaccg tcatatattc
     1621 ctatttgcag gcgatcggtg tgctcaacgc gcacggaaaa gaatgcggct tctgtgcgca
     1681 ggagccgctc atggccgcgc aagagcctga cgcgttttcc tgacatcggc ataaaaacaa
     1741 agacgccgct ttcccaacgt gttttgggaa agcggcgttt gtttgccgaa aggcggtcag
     1801 tgtccgcagt gcccgccgca ggtatgcccc tcgccgtggt ggccgtggca gccctcgtgg
     1861 ccctcgccgt ggtgttcgtg gtgggcgcag gtggtgtccg ggtcataggc cagcgtgccg
     1921 gccagcagcg cttcggcctg tgcgtccgcc gcgccgcgcg cgccggggta cagcgcaatg
     1981 cccgcctcgg ccagcgctgt gcgcgccccg ccgccgatgc cgccgcagat cagcgtgtcc
     2041 acgccgtgcg ttttgagaaa caccgccagc gccccgtggc cgctgccgtt tgtgtccacg
     2101 acctcgctgg aagtgatctt gccgttctcc accgtgtaca gcttgaattg ctgcgtgtgg
     2161 ccgaaatgct ggaataccag cccgttttca taggtgacag cgatcttcat atttgcatcc
     2221 tccgatatgg tttgataccg tgcgcagaag gccgagccgc ccgcgctctg tgcgcaccgc
     2281 ttgccgcggc agccgcccgc ccgggggcac agctcatagg ggccgccctc cacatgcagc
     2341 agccgcccgt tcaccagcat gtccgccagc ttggcgcgcg cggcctcgta cagggcctgc
     2401 actgtggttc gcgccacacc catctgcccg gcgcattcct cctgcgtcag gcccaaaagg
     2461 tcgatcaggc ggatggtctc gtattcatct actgccagga cgacgttttc ggcaggcgcg
     2521 ccgtccagcg ggccaaagcg ggcgttgcgc ggcggctcgc agacgcgccg gcgctttaca
     2581 ggacgcggca tggcaaagcc tcctttgttt ggttctgcgg ccagtataac attataaatg
     2641 gcatatgtca ataacttgcg cgcccttttc cggaaaagcc gccccagtgc cggcgcgccg
     2701 ggtgcgtttt tttagcgcgc cgggcatggg agggcgcatc ctcccgcatg gcctctgcat
     2761 cctccgtgtc ccgggcgtcg cggggggcgg ggaacagacg gttgatggcc aggccggcca
     2821 acaccccgac gccggtatca aggatgcgcg cgatggtata ggagacgtat cggtcctttg
     2881 tgacggtgaa catcaccaca aagtacacca cggcggcggg ctggatggca ctgtgcgcgc
     2941 cgaacaggag attgatgtaa atgacgcaga agaggcccag gaccaggtaa aggtgccccg
     3001 gcaggccgaa gggccggttt gtgtacagcc agtaaaacgg gatcaccacc agcccgccca
     3061 gcagcgtgcc cagaaagcgg ttgccgccgt ggcgcacgcc ctcgcgccag aagctgccca
     3121 tgccgaacac cgcgccgatg caggcgaagc aggggttgcg cccgcccagc agcccgtatg
     3181 ccgcacagac cagcagcacc gcgcccagcg ttttgaagat gcgcatgccc accttcggca
     3241 gcgcggggaa atgaaatgaa aaatgcatgt tgctgcctcc taaccttctg cggggcgcgc
     3301 gcggtagcgc agcggcgaaa ggccgtattt ctttttgaac agccgtgaaa agtacgccac
     3361 gtccaccatg cctacccgtg cggccacctc ggccacgggc aggtcggttt cgtccagcag
     3421 acggcgggcc gtctgcaggc gcagcgtatg gatataggct gtcagcgttt caccgcgctc
     3481 ccggctgaac tgggcggaga gatacgtggc gttgaagccc agcgccgccg caatggagcg
     3541 cagcgaaagc tcgccggaaa ggtgcaggct gatgtagctg agcgttttct gcacggcctg
     3601 ggagcagccg ctgtgcgcgt cgttgcgcac caggcggcag tagcggcgca gcatctccag
     3661 gcccagacgg ttcgcttcca cggtgtcggc cacgcgttcg atcttcaccg ccagcgcgcc
     3721 ggagagctgg tcgatataca ccggatgtac gccgccgctt tccgcggcct tgcgcagcag
     3781 cgtgttgacg acgatgagca tgtttttgac gtcccgcagc agctcgccgg tgcggggcgt
     3841 gaaatttttg atggggaacc gccccagatg gcgcacggcc gcgtcgtaat cgccgcggga
     3901 cacggcggcc agcagcgcgt tttcatcgcg gtagcgccgc tccagaagct ccatggaaaa
     3961 ctggaacgcc tcgtccggct gcgcccggcc cgtcacggtg gaagccagcg tgcggtgcac
     4021 cacgcgggcg gggtcgtgcg cgccgtacag ctggcatgcc acggcgtgtg ccgccgcagc
     4081 catcgtgggc tcggccacct cgggtacggt gccgtaaaat gtgcgcagcg cggcggcgtc
     4141 gcccttgccg atgcggttct gcgccagcac acgctcaaag aacgggccgg acgccgcctc
     4201 atacagaaaa gggccgaaca gcagcacgct gcgcggcttt gggccgggca caggcatgaa
     4261 aacatagttc agctccagcg tattgtgcac atagtacacg gtgccttcct ccagcccgtg
     4321 catggcgctt gcgctgaaaa tgtccttgta atcaaaatcc gcgcgcagaa tgcgggacag
     4381 ccggtagtcg aactcctgcg cgtccttgta gggcgggcgc agcagatgcg cctccacgcc
     4441 cagcgcggcg gccatcatat cgcggatgaa cgcaaaggga atgtctgcca taaatcatga
     4501 agctcctgta aaatttttat gatatttgaa tgttacccaa aaattgtaca aatgtcaata
     4561 aaatcttcct aacggggcat ccttggtata tgttatcttg ataaaggagt agttcgcata
     4621 tggcattgca gcctcaaaac gagcgcccgt tcgggttccg cgataaattg ggatatctgt
     4681 tcggggattt cggcaacgac ttcacattta ttttcgcggg cagctatctg acactgtttt
     4741 atacggacgt gctgggcgtc agcgcagggc tggtgggcgt gctgttcgtg gtggcgcgct
     4801 gcgtggacgc gttcacggac gtgggcatgg gccgtctggt ggatacgctt ccgccgtccc
     4861 gcggcggccg gttccggccg tggatcctgc gcgtgtgcgt gcccgtggcg ctggccagcg
     4921 tgctgatgta cctttatttt gcacgcagct ggccctatgc gggcaaggtg gcttacatgt
     4981 tcgccaccta tattttctgg ggcagcatct gttataccgc catcaatatc ccatacggtt
     5041 ccatggcgtc ggtgctcagc gccgacgcag gcgagcgcgc gtcgctctcc acgttccgca
     5101 gcgtgggcgc catgctggcg aacctgatca tcgccgcggc gacgccgttg tttttgttcc
     5161 gcaccgcggc ggacggcacg cagaccgtta tcccggagcg cttcacgctg attgccgttg
     5221 tctacggcgt gtgcgccgtg gcctgctatc tgctgtgtta tgcgctgtgc cgtgagcgtg
     5281 tgcaggcggc gccgcccagg gaggccaaac ggcagagctt tgcctcgctg gcaaaggcgc
     5341 tggcgggcaa ccgcgccctc acagccgtca tcggcgccgc gctgctgctg cttctggcaa
     5401 gcctgctggg ccagggcatg aacatgtacc tgttcaaaga ttatttcaac agtgcggaca
     5461 tgctctcgct tgtggggttc gcgggcgtgc tgcccatgct ggcgctggcg ccctttgcgg
     5521 caaagctgtc caaaaggttc ggcaaaaaag aggccggctg tgtgggcgtg ggcgttgccg
     5581 ccgcggcgta cctgctgatg tgggcgctgc gcctgcgctc ggtgtgggcc tatatcggcc
     5641 tgatgctggt gggtagcgtg ggcgtgggct ttttcaatat ggtcatctgg gcgttcatca
     5701 ccgacgttat cgattatcag gaggtacgca ccggcagccg cgacgacggc accgtgtacg
     5761 ccgtctactc gtttgcgcgc aagctgggcc aggcgctggc gggcggcgtg gttggctttg
     5821 cactggcggc catcggctat gtgagcagca cgggccatgt ggtgcagacg cagcctgtac
     5881 gcgagggcat ttacaccatt tccacgctgg tgcccggcct gagctatctc ggcgtgttcc
     5941 ttgtgctgct gctggcctat ccgctcacca aaaagcgcgt ggaggaaaac gccgccgtgc
     6001 tgcgcgaacg gcgcggcgaa gcggcacagg agccgtagcg gccccggccg ggcgcacggc
     6061 ccgcgccgca tacgatacag ctttccgccc ggtttttccg ggacacatat atatcatcag
     6121 aaaagagagg gaaaacatat gaaacacatc ctacccatca acgagggctg ggcctttgtc
     6181 aaaggctgcg aaacggcccc ggcagccatt cccgccggcg ctgcccgcgt ggacctgccc
     6241 cactgctgga acgcattgga cggccaggac ggcggcagcg actactggcg cggcccctgc
     6301 tgctatctca agaccattga gaagccggcg ggcaaaaacg tgtatgtgga agtcgaggcg
     6361 gcttccagcg tgggcaagat catcgtcaac ggtgtggaga aggccagcca taagggcggc
     6421 tattcgctgt tccgcgccaa tgtgacggac gcgctgcacg acggcgagaa cctgctggcc
     6481 atctgggtgg acaacaccca gcgcgacgac gtgtatcccc agacggcgga cttcacgttt
     6541 tacggcggcc tgtaccgcgg cgtgaacctc atcaccgtgg aggactcccg ctttgacctt
     6601 gactattgcg gcggaaaggg ctttgacgtg tccgctgcgg tggaggacgg cgcggccgtt
     6661 gtctctctgg acgcgttcat cacaaacccc gtcgagggcc agagcgttga gttcatcgtc
     6721 cgcgacgccg cgggcgtcat cgtggccggc gccgtgcgtc cggctgcgga acgggtgcac
     6781 gcggaggtgc gcattgaaaa cgcccacctg tggcagggcg tgaaggaccc gtacctgtac
     6841 gccgtggagg cactgctcac cgttcacaac gagaccatcg acaatgtgtc cgccaacctg
     6901 ggcgtgcgca cctatacggt ggacccgcag aaaggctttt tcctcaacgg cgtgctcacg
     6961 ccgctgcgcg gtgtgtcccg ccatcaggac cgtctgggcc agggcaacgc gctgacgaaa
     7021 gagcagcacg aagaggacgt ggcctttatc cgcgaggtgg gcgcgaacac cgtgcgcctt
     7081 gcgcattacc agcacagccc gtatttctat gacgcgtgcg accgcgcggg ccttgtggtg
     7141 tgggccgaga tcccgttcat ctctgtgatg aaccccgccc ccggcgcgca cgagaactgc
     7201 cgcagccaga tgaaggagct gatcgtgcag aactacaatc atccctccat ctgcttctgg
     7261 ggcatttcca acgagatcac catcggcggc gagcgcccgg gcctgctgga caacctgcgg
     7321 gacttgaatg ccctggcgca cgagatggac aagacccgca tgaccaccat cgcccacgtt
     7381 tccatggtgc cgatggagaa cgatatgcac catatcaccg acgtgctcag ctataaccat
     7441 tatttcggct ggtatggcgg cgagctggaa aacaacgagc aatggctgga taaattccac
     7501 gcgctgcatc cggaccgccc gctgggcatc tccgagtacg gcgcggaggg cattgtcagc
     7561 taccacagcg acacgcccaa gtgcaaggac tacaccgagg aataccaggc ggtgtaccac
     7621 gagcatctgg cgaaggtcat cgacgagcgc ccctggctgt gggccacgca tgtgtggaac
     7681 atgtttgact ttggctgtga cgcccgcgac gagggcggcg taaagggccg caacaacaag
     7741 ggcctgatga cgctggaccg caaaattcgc aaggacgctt tttacctgta caaggcatac
     7801 tggagcgacg aagaatttgt acacctgtgc agccgccgct atgcccagcg caccggcgaa
     7861 gaaaccacgg tgaaggtgta ttccaacctg cccaaggtcg cactgtatgt ggacggcagg
     7921 ctgtttgccg agcaggaggg cagcaaggtg ttcgtgttcg agcatgtgcc catgagcgac
     7981 ggctttacgc agatcagcgc cagggcgggc gcgtgcgccg acgcgatgtc cattgaaaag
     8041 gtggccgagc cgaaccaggc gtatatttat gttgacccgg acgatacagg cgacgacgcg
     8101 ggcgcggcca actggttcaa tgtggacgat tacaaggacg tggacaccat tgtggtgcgc
     8161 gaggggtact tctccgtcaa ggataagatc ggcgacatca tgaagaacga agaggccggc
     8221 aatgtgctga tgcagttcat gcaggccacg gtaaagatga agctgaaaaa aagcatgctg
     8281 ggcatggtga aggacatgac cctggagggt atggcaggca tggccagcag catgggcatc
     8341 ggcggcaagg ccggcgcgga ccccgagcag ggcaagcgca tgctcatcac gctcaacgag
     8401 atgctgaaca agatcaaaaa gtaagcgcgt gccgcacgcg cgcagagccg ccg
//
LOCUS       DAWBSB010000088         8420 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1795_length_8420_cov_2.389480, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000088 DAWBSB010000000
VERSION     DAWBSB010000088.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 8420)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8420)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8420
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1795_length_8420_cov_2.389480"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2343..2352
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1735 a         2407 c         2493 g         1775 t
ORIGIN      
        1 ctgcgatatg ctgcgcatcg gcatgctgcc gatgacctgc atgggcagct acgagaaggc
       61 catggacttc gccaaggagg cggacgagac cgccgccagg ctgaaggaag agggcatcga
      121 tctgtactac cacaaccacc atgtggagtt cgcacgctac aacggcaagt atctgctgga
      181 catcatcaag gacaacacca aaaacctggg ctttgagctg gacatccact ggattcaccg
      241 cggcggcgag aatcccgttg agtttatcaa aaagtacgcg ggccgcatcc gcctgctgca
      301 cctgaaggat taccgcatcg gcgaaatgaa aatgcccgag ggcggtattg atttcaccaa
      361 tcgtgagtcc attatgaagt tcatgggcgg gttcaacaac gtggtgcagt tcgccgaggt
      421 gggcgagggc acgctgcccg tggccgcctg catcgaggcg ggcctggccg gcggcagtga
      481 gtatttcctg gtcgagcagg acgacaccta cggccgcgac ccgtttgaga gcctgaaaat
      541 cagccacgat aacctcgtca agctgggcta cggcgagtgg ttctaagcgc ataaacggtg
      601 atttttccgc ccatgctttg cggacgggaa aaacagcagc cctccggccg gttcgtccgc
      661 agccgggggg ttgtctcttt tcggggcgcg caagcattgc aatgcaagaa gcaataatag
      721 caatttctat tacaaatccg tatggtaaac ttgagacagc agataaaagg gccggtgttt
      781 tgcggtgttc ccgtgtgcaa aatgcggcgc ccggaataaa atgaacagga ggaacgatcc
      841 caatgaaacc ctttatggac aaggattttt tgctttccac tcccacggcg cagaagctct
      901 atcatgaata tgcggcggat atgcccatca tcgactatca ctgccacgtc agcccgcggg
      961 agatcttcga ggaccgccgg tttgagaaca tcacccaggt gtggctgggc ggcgaccatt
     1021 acaaatggcg tgtgatgcgc tccaacggcg tggaggaaaa gtacattacg ggcgacgcgc
     1081 cggaccggga gaaattccag aaatttgccg aggctctgcc tcgtgccatc ggaaacccca
     1141 tgtaccattg gtgccatttg gagctgaaaa agtacttcgg ttatgagggc gctctgaatg
     1201 gagaaacagc cgaagaggtc tggaatctgt gcaacgaaaa gctgaaggag ccgggcatga
     1261 gcgtgcgcgg gctgatcgcc cagagcaacg tggcctttgt cggtaccaca gacgaccctg
     1321 tggattctct ggaatggcat cagaaaatca aggaagatcc gtccattacc actgtggtgg
     1381 cgccgtcttt ccgcccggac aaggctctga acatcgacaa ggcgggttgg aaggagtata
     1441 ttgcgaagct ggccgaggtg tgcggctgcg agatcaaaga cagcgccgcg ctggagtgcg
     1501 cgctggcctc ccgcatcgac cattttgacg ccgcgggctg ccgcgcgtcc gaccacggcc
     1561 ttgactatgt catttaccgc ccggcggacc gcgctgcggt ggatatggtg ttcagcaagg
     1621 ggatgaacgg cgaagccctc accgtggagg aggtcgagct gttcaagacg gatctgctgc
     1681 tgttctgcgg ccgtgagtat gcaaagcgcg gctgggtgat gcagctgcac tataattgtc
     1741 tgcgcaaccc caacaagaaa atgtttgcga agctcggccc ggacaccggt tacgacgtga
     1801 tcaacactgt caattgcggc gaggccgtgg ccggtgtgct gaacgcgctg gaggaaacag
     1861 gcgagctgcc ccgcaccatt ctgtacagtt tgaatccggc ggacaaccag ctgctggata
     1921 ccatcatcgg cgcgttccag ggcactgaag tggcgggtaa ggtccagcac ggcagcgcct
     1981 ggtggttcaa cgatacaaag acgggcatgc aggagcagat gaccagcctg gccaacctga
     2041 gcctgttggg caacttcgtg ggtatgctga cggacagccg cagcttcctg agctacaccc
     2101 gccacgaata tttccgccgc attctgtgcg aactgatcgg cggctgggta gagaacggcg
     2161 agtatccggc cgatatggag atgctgggcg gcattgtgcg cgatatcagc tttaacaatg
     2221 ctgcaaggta tttcggcatt ggacgctgaa gccaatatat tgcgaatatt gcagaaggtt
     2281 tttcgtcgct ttaaaaggag ggctccttag ggagctttcc ttttttattg cgtccttgta
     2341 aannnnnnnn nnattgcgaa tattgcagaa ggtttttcgg cctacaacaa ggaccgtcct
     2401 gtgagaacag gacggtcctt ttcaatgctg ccttccggcg gaatggtcaa agcaaaactc
     2461 cttccaggcg cagcagtgct tctttcttat ccagccctcc cccgtatccc gtcagctgtc
     2521 cggatgcgcc gataacgcgg tggcagggca cgaagatgga aagcggattg cgcccgttgg
     2581 cgcggcccac cgcccggcag gcggcaggac ggccgatctg ccgggcgatg tccgcataac
     2641 tgcgtgtttc tccatagggg atggtggaca gcgcctgcca gaccgtacgc tcaaagggtg
     2701 taccgatttc aaactccagc ggcagctcga actccgcggc cttttgcagc aagggcgttt
     2761 cccgtatctc cgcgtccgcc gggcgttcgc cgtgcagcag cacgtctgta acggcgcggc
     2821 cgttttccgc gaggcccagc ctgccgatgg gagtatcgta atagaaaacc gatttcatgg
     2881 cattcgttcc tttctgtcct tcgtatggag cgtctgccct cattctacca tgttttgcgc
     2941 aaaaaatctt ccagtttcgg cgcaaatcgt ttatactata caattgaggt gataccaatg
     3001 aaactgttgt ttgcaagcga cctccacggt tcagcctatt acgcagaaaa gctggaggag
     3061 ctgattcgga acgaggcgcc cgacaagact gtcctgctgg gcgacctgct gtaccacggc
     3121 ccccgcaacg acctgccccg ggagtatgat cccaaaaaag tgacggccat tctgaacggc
     3181 ctgaaaactc aggtgctggc tgtgcgcggc aattgcgacg ctgaggtgga ccagatggtg
     3241 ctggagtttc ccatcatggc ggattacatg gctctgtttc tggaaggacg catggtgttc
     3301 gttactcacg ggcatttgtt caacgaggaa aatctgccca atttcaaccc gggcgacgta
     3361 ctcatccacg gccataccca cgtgcgtgct gcggcggacc ggggcgctta tttcttcctg
     3421 aaccccggaa gcatggcact tccaaaaggc gacggcgttc acagctatat ggtgtatgag
     3481 gacggcgtgt tcaccacgaa ggatatggac ggcgcggtgc tggaaacgct ggacatccgg
     3541 cgctgaccca tggaaaagac ggtagagata gagcgcagca tcatcaaaaa atacaagaaa
     3601 cccatttggc atcggttcat cggcggctgt aaggactatc ggctggtgaa cgcgggggac
     3661 cgcatcgcgg tgtgtatttc cggcggaaag gacagctttc tgcttgccaa atgcatgcag
     3721 cagctgcaaa agcacagcga cgttccgttt gaattggaat ttttgtgtat ggacccgggc
     3781 taccgcccgg aaaaccgcgc catgatcgag cgcaacgccg cgctcatggg ggtgccgctg
     3841 catattttcg agacggatat ctttgacaca gtggtgaata tcgaacagtc gccgtgttat
     3901 ctgtgcgcgc gcatgcggcg cggacatctg tataaaaacg cacgggcgct gggctgcaat
     3961 aagatcgcgc tggggcacca tttcgacgac gtgatcgaaa cgacgcttat gagcatgctg
     4021 tggggcgcgg aggtcaagac catgatgccg aagctgcaca gcaccaatta tccgggtatg
     4081 gagcttatcc gtccgctgta cctggtgcgt gagacggata ttttggcgtg gaaacgctac
     4141 aacggtctga catttctgca gtgcgcctgc cgtttcaccg agcaggaagc ggatgaaggc
     4201 atgcacggcg cacgcagcga aacaaaggct ctcattgcgg cactgcgcaa aacgaatccg
     4261 caggcagacg taaatatctt ccgaagcatg catcatgtca atttgaacat ggcgctggga
     4321 tggacgcaag acgggcagct ccgcagcttt ctggacggct atgaaacaca gcgcactgcg
     4381 gcgggcggcg ggccggaaaa cgagtgaaca ggcacgaaaa aatcccggac ggtattggac
     4441 catacgtgcc attccgtccg cttcgcggcg ggcgctgcct gtgcagatga ctgccgtgtc
     4501 agcatgggca aagacaaaaa acagcaccgc gccccgcccg gttcctgccg ggatgggcgc
     4561 ggcgctgttt cgcgcagggg aaaagggcgc acgcgggcgg cagcgtgcgg cggtcgaggc
     4621 tgcacaagac gtacggaaag gtgggcggca gccgcttgcc cggcggactt cacagtggcg
     4681 ccgcaggcgc gaaaacggag ttcacgccgt cagtgccgcg ggctcaacaa ggctccacac
     4741 gccgttttcg cgtaccagtg caagaccatc cggcgtggga caggcgtatt cgtagcggca
     4801 gggaacggct tccgcgccgt cggcattgag gaagccccaa agcccgtccc ggcacacggg
     4861 ggcgtagccg ctgcgaagcg ggtagccgca gcccgggcgg gcatacacgg cctcgtctgc
     4921 ataagtggct gttttttcgt ccagcacgct ttgtgtggcg cagcgagccg cccacacgcc
     4981 gtcgtaaaga aaatccgtca cggcccggcc ctcggcgttg agataggcgg tcttgccatt
     5041 cttatatacg gagccgagtc cttcccgaaa ccagcccgca gcctcgtagg cttcttcggt
     5101 gagcaggtgc ggcgcatccg cctcggcggg cccggcgaaa ttgtagcaga aagcgctgtc
     5161 gatgggctgg ggatacagcc cgcccgtcat ctcgctttca gccatgacgg aaagctgaac
     5221 ggggaggaat acgccgtatc gctcataatc ggcggcggtg acaggagaag gggcttgtgc
     5281 ggcttcaccc gtccggcaga agccgggcgc ggatgcggcc acgtcccaat aaaaaagtgt
     5341 gccgctgccg ccgtgcccca cttccagtgc gccgtccccg gtttcggaca agcgggcgtt
     5401 gactgcatcc agttcatccc agttcattcc gtcgctgccc catgaccagt gccccagatg
     5461 gcaccgcgtc acgggctgcg gtgcgcggca ttccagcaca acggtgaaat cttcccgcat
     5521 caggccccac aggccgcctc ggcacatggt gtaatacaca ggtccgctgc cgtcaaagcc
     5581 gcaggcaagg tacgggtacg gtgtggcatg atccgccaca ggctgaatct tttcataggg
     5641 aaggtccaaa ggcggcgctg cctgtccggc cgtgccggaa gccggcggca gggaggaggc
     5701 tgcggcggaa gaggatggca cggaggcgga agaagcgccc ggctccggcg cacacgcggc
     5761 cagaacaaag cacagcgcca gcgcggcggc acagacatgt tttttcatag aagctcctcc
     5821 ttgcgtggat gtttcccgga tatgtccggc tctgtctgct gtattcaata tagacgtttt
     5881 ttgtatccgc gttgtaacga aagcagtgga aaccaaaaaa tggctgtcgc aaaatgccct
     5941 tcaaaagcac tttgcgacag ccattttcgt tggcggccga accgtatttt agccgatctc
     6001 caccatgacc ttggtgcctt cgcgcacggc ggcagcacgc atgaccacgc ggatcgcctt
     6061 ggcgatgcgg ccctgcttgc caatgacgcg gcccatgtcg tcctccgcaa cggtgaggtg
     6121 atacacgatg attccgtcct cattggcctc gtccaccgta acctttacgg cgtccttgtc
     6181 ttccacaagg cctttggcga ctgctaataa tagctcttcc attgtgtgct ccttcacgat
     6241 tacagaatct cgttcttctt cagcagaaca cgaacggtat cggtgggctg agcgccggtc
     6301 gcaatccact tcttcgcctt ctcagcgtcg atctggacaa cagcgggctc cttggtcggg
     6361 tcatagtaac cgatctcttc gatgaaacgg ccgtcgcggg ggaagcggga atccgcaaca
     6421 accacacggt agaacggagc cttcttcgcg cccatgcggc gcagtctgat tttaacagcc
     6481 aatcctgtca cctcctgtaa ttttttaaaa actgtatatc agcggggcaa agccccgtga
     6541 ttaccgaaat aaaagcggcg cttagcggaa catgccgccc aggcctttca tgccgcgccc
     6601 aaaccccttg gggttctttt tgaactgctt catcatcttc tgcatctgtt catactggcg
     6661 aagcaggcgg ttaacgtcct ccacgcgcgt tccgctgccg gcggcaatgc ggcgcttgcg
     6721 tttggggtca atggtagagg gtttggcgcg ttcgcccttt gtcatgctct ggatgatagc
     6781 cttcatgcgg ccaagctctt tttcgctttt gtccagctcg tcgtccgaaa tgcgtcccgc
     6841 gccggggaac atggacatca tggcggcagc gccgcccatt ttctttacct gttcgaactg
     6901 atccagcata tcgttcatgt cgaatttgtt ctgcatcagg cgcttggcgg tttcttcggc
     6961 ctttttctgg tcggcggtgt cctgcgcttt ttcaatcagc gtcagtacgt cgcccatgcc
     7021 cagaatgcgg ctggccatgc ggctgggatg gaatacctcc aggtcgtcca gcttttcgcc
     7081 cacgcccgcg aatttgatgg gcttgcctgt aacggcacgc acggaaagcg cactgccgcc
     7141 gcgggtatcg ccatccagct tcgtcaggat aacgccgtcc aggccggtgc tttcagagaa
     7201 gcttttggct acattcacag cgtcctggcc ggccattgcg tcgatgacga gcagcgtttc
     7261 gtccacctgg acgttttcct tgatgcgctg caattcggcc atcaggtccc cgtccacatg
     7321 caggcggccc gccgtgtcga ggatgacgat gtcgttcccg tagtcctttg cgtgggccac
     7381 ggcctttttg gcaatggcct cgggcttttc acccggaagg gcgaacaccg gcgcaccggc
     7441 cttttcgcct acgatcttca gctgatcgac tgcggcgggg cggtaaatat cgcatgcggc
     7501 gagcagcggg cggtggccct ccttgataaa gtgtttggcc agcttggcgg tatgtgtggt
     7561 tttgcccgca ccctgcaggc cgcacatcat gatcaccgtc ggcggcttgg acttgatgtg
     7621 aatgcgcgcg gcctcgctgc cgcccatcag ttccgtcagc tcatcgttga cgatcttgat
     7681 gacctgctgc gcaggggtca ggctctccat gacctcctgt cccatggcgc gctgggtgac
     7741 gccggcgata aaatctttcg caactttata gttgacgtcc gcttccagca gggccatgcg
     7801 cacctcgcgc atagcgtcct taatgtcctg ctcggtcagc ttgcccttgc cgcgcagctt
     7861 tttgaatacc gaagccagct tatcgcttaa actctcaaat gccatcggcg gcacgcctcg
     7921 ctttccgttc gttatgcagc gctgttcacg cctcgctcat gcggttgatg atatgcagca
     7981 tctcgtccgc agcgcggtgg atgtcctcgg acatcgcgta attgttgttg ttgtagaaat
     8041 tgatgtcgcg ggcgagctgc tccagcgatt caatgccctc ctgtacagcg cggtaccgcc
     8101 gcgcaaagcc gaccttgtcc tccagctcca ggatcgtacc ctcgccgcgc ttgatggcgt
     8161 cgcgtacacc ctggcgggtg atgccgaagt tgtccgcgat ctcagaaagg gagaggtcgt
     8221 cgttgtaata ctgctccatc acgtcgcgct gtttttccgt cagcacgtcg ccgtaaaaat
     8281 caagcaggta cgaaatttcg aggttttttg taaccaccgc ggccgctcct tttctcaaaa
     8341 tcattgtgta aagcaaaccg ctttacacag cttgttcatt ataccgcaca tgcggtgtaa
     8401 agtcaaggcc ttttcagcgg
//
LOCUS       DAWBSB010000089         8379 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1802_length_8379_cov_3.513815, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000089 DAWBSB010000000
VERSION     DAWBSB010000089.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 8379)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8379)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8379
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1802_length_8379_cov_3.513815"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1691 a         2437 c         2647 g         1604 t
ORIGIN      
        1 ttccaagcag cagccttgct atggcggaga gcgcgggttt tacggcaaag gccgcaaacg
       61 ctttataagc gatttcaaac gctgtcaggc cgggaaggat gcgcaaaaaa acaagcgcag
      121 taaggcgtat tgttggccgc aatggacagg ataaggaatg cataaacact tctccagaca
      181 actgttcgcc gcggcgcgca aagtggataa acacggcaag tatttttatt tttacccgga
      241 aaaattatac catgcgcgcg gataaaaaga aagcacggct tgtcctgtgc cggaaaagtg
      301 catccgccgc atagggtgag gacaagggtc tgtcccgggc gcagagcagc gcagggggaa
      361 aaggcccggg aggcgtccgc ctcccgggcc ggatgcgcga tacggctgcg cgggatgagc
      421 gccgccttac agcgtggcgt tgaatgcggg gatgagcgcc gcccaggtag cgctgcctac
      481 cacgccgtct accggcaggc cggcggtggc ctggaacaga cctacggcac gggtggtgtt
      541 ctggccgtaa tttccgtcca ctgtcagcag aggacggccg cttatgccgt tcagatagct
      601 ctgcacaaag cggacgtaat cgccggtgga gcctacgcgc agcggtacgc cgggatactg
      661 tgtggtaacg tgcgtggggt tgcccgcctg catggcgtta tgcacggaga cgatcttgtt
      721 ccaggtatcc ttgcccagaa tgccgtcggc ggaaaggctg aactggcgct ggaagcggcg
      781 cacggcgttg gtcatattgt ttccgtacgc gccgtccacg gtctgcgtgt tgatgccggt
      841 gtagatgcgt gcaacttcgt tcagacggcc ctgcatgtgg cgcacatctt ggctgcggtc
      901 gcccgggcgc aggggaatgc cgggataggt gtcggcgctg ccgccgatgg tggcattgta
      961 atcgctgacg atggcgttcc aggtatcgtg gccgatgacg ccgtccatcg aaagacggtt
     1021 gatcacctga tactgcatca cggtgctggc ggtggcggag ccgtaccgtc cgtccacgac
     1081 cagacggttg agggtggggt acttggcgtc gcgcaggccg ttcaaatagg tctgcatccg
     1141 cgcgacttcg ctgccggagc tgccgggccg caaagcggtg ccgggataga cgggatcgac
     1201 tacggcgagg actgcattat tattttcaag cataaaaatt gcctccttct gccccatggt
     1261 atgcgcgcgg cgtgcgccgc gtgcgggggc ggaaaggagg ctgttttgcg ttacagagct
     1321 tttttgcggt gcgtttccac agctttcagc agaaaataca gcaaagcggt gtccaaaggc
     1381 agcttaacgg catttttgat aagccgcatg ccggaaatca cgaaagcgtt gccgtacatg
     1441 atattcagcc acagcggcgt gagcaccagg ttgatgacga gcaccgcgga caggcaggcg
     1501 cagaacgtgc gccacaggga tacgggtttt ttgtacagcc agcagccgta taaaaagccc
     1561 agcaggaact cgttgagcgt gaagccggga aaaaagccgc cgttgggccg caaaaagtag
     1621 cccgccacgt cggtgacgat gcccattagc ccggccagcc acgggccgta caggagcgcg
     1681 cacgttcctg cggccagaaa ggaaaagccg atctccagaa actggctgac cgcgatggta
     1741 aactggttga gcaccaggct cagcgccgcc agcatggcgg ccccggccag actggcggtg
     1801 cgccgcacct cgcgggcggc gtcggacagg cggcggggaa agccgcgcag ggaatcgaat
     1861 gtggattgaa aagcagacat gaaaaaacct cctttatttt ggcgttgccg cataaaggag
     1921 gcaaaaggcc cgcgcacagc cgcacggcgg cgcgcttgcg cgcggctccg ggcggccctg
     1981 gcaggcccgt gttgcattcg tcatgcagca gcgggatgcg aagcctgcac cgcaagcggt
     2041 gcttttcgtc cgttccgcaa ctccccgtcg gaacggcact gcggcccttt ttgggggcca
     2101 acgcgcaggc ttctactctg cgatagggtt attataggac ggcggaagga aaattgcaag
     2161 tatccgcgcg ttttttcttt caaagcgctg cgccccatga tacaatacag gcagaatgaa
     2221 aaatattttg ggcggcgggc cgcttgcggc cgcgctgtct gcggaaagcg gggcagtcgc
     2281 atggaaaaga tatcgcgcaa aaactgggcg ctcatctggg tgctgggaat ggcggggcag
     2341 atctgctgga acatcgaaaa ctcctggttc aatacctttg tataccggaa gatcgcgccg
     2401 gacccgtcca tcgtggcgtg gatggtgggc gtttccgccg tggtgtccac cttctgcacg
     2461 ttcctcatcg gtactgcaag cgacaggctg ggcaggcgcc gcccgttcat cgcgttcggg
     2521 tacatcctgt ggggcgtgtt caccatcggc ttcggcgtca cggagttcct gccgaaagat
     2581 ccgctgtggc tcgcggccac ctgcgtggtg ctggcggacg cggtgatgag ctttttcggc
     2641 agcatgggca acgacggcgc gttcaacccc tggacgaccg atatctccag cgcaggcaac
     2701 cgcggcaagg tgggcggcgc gctggccgtg atgccggtgt tcgccaccat tttcggggcc
     2761 attgtgtcgg gcattatcat tgacgcgctg gacttcttcg cgtttttcac catcatgggc
     2821 ggcatggtca ttgctgtggg cgtgctgtgc ctgttcacgc tgcgggatgc gccgggcctc
     2881 gcccccaacc gggacccacg gggcttttgg caccagttcg ccgctgtgtt cgattataag
     2941 atcgtgggcc gcaaccggga gctgttctgg gtgtttctgg tgatgatggt gtatttcatc
     3001 ggcttcaacg tatatttccc gtacatcacg gtttacttta ccgacaacct gggtatggac
     3061 tacacgctca cgggcgtggt gcagggcgcg ggcctgctgg ccgcgtcggt gctcaccatt
     3121 cccgccgccc gcttcatcga ccgcggcaag tgcgggccgg tcatcgcggc ggccgtgggc
     3181 tgtaatctgc tgggccttct gggcgtcagc ttttccacgg cgttcgttcc gctgcttatc
     3241 ggtgtgttcg gcgccggcgc ggggtatatc ctggtgctgc aggcgctgac agcgtggatc
     3301 aaaaacctgt atccggagga ccagcgcggc cagttcgagg gtgtgaagca gctgttcttt
     3361 gtgtgcgtgc ccatgatcgt cggcccggcc attgccaccc ctgttatcaa tgctttcggc
     3421 gttgaaaaaa ttgtcaacgg cgtggcgggc atggtgcccg gcaacagtct gtttctgatt
     3481 tcggggctgg tgacgctgct ggcgcttttg ccgctgctcc ccgccgcgcg catggaaaaa
     3541 gcgcgcgccg gcgccgcggc gcagccgtaa aatgcagacg gcccggccca ggttcttttg
     3601 gagggcgccg ggcgttttgc atattctctt tcccgccgct ctatgataag atacaattgt
     3661 aatgcaaggc gcggcccgtg cgccgcgaaa aaggagttgt ttggatgaaa tttgaacaga
     3721 tgcagtacgc tcgcccggac tttgacgcgg cacgggaaac ctttgcgtcg ctgcgcgcgc
     3781 gcatcgccgc cgcgccggac gcgcaggcgc agctggaggc gttccgggaa tttgagacgc
     3841 tgagccgcca tatctccacc atgtccaccc tggctgagat ccgccacacc gtggatacgc
     3901 gggatgcgtt ttacgaagcc gagcgggaat tttttgacgc aaacagcccg gcgctgggcg
     3961 aagcccagct ggacgtgtac cgcgcgctgc tggcctcgcc gttccgcaca cagctggagc
     4021 aggcgctggg caggctcact tttgaaaaaa tggaggtgga cgtcaagtcc tccgacccgg
     4081 ccattctgga gctgatggcg gaggaaaacg ccctcaccac ggcttatgaa aagttgtacg
     4141 cctcggcgct gatcgaattt gacggcagaa aaaataccgt cagccagatg agcctttaca
     4201 agcaaaaccc gggccgagct ggacgaattg tacgataagc tggtgaaaaa ccgtacggcc
     4261 caggcacgaa agctgggtta tgagaatttc atccccctgg gcgccatccg catgcggcgc
     4321 atcggctaca cgctggagga catggccgcc taccgtgcgc agatcaaaaa agatttcgtg
     4381 cccgtggtgg cggaactgaa gaagctgcag tatgcccgca ccggcgtggc ggacccgaaa
     4441 ttctacgacg acgcgttctg ctttgcggac ggaaaccccg cgccccacgg cacgccggag
     4501 gaaatactgg ccgcgggccg cgaaatgtac catgccctca gccccgagac ggcggaattt
     4561 attgacgaaa tgttcgacgg aggcctgttc gacgtgctct ccaaggaggg caaggccccg
     4621 ggcggctact gcacctacct ggcggattac aaagcgcctt tcattttctc caacttcaac
     4681 ggcacgtccg acgacgtgga cgtactgacg catgaagcgg gccatgcctt tgccgcgtgg
     4741 gtggccgcgc ggaaagacct tcccatgatc ctggaggagc ccggcatgga gagctgcgag
     4801 atccactcca tgtccatgga gttccttacg gcggagcatc acgaaaaatt cttcggcacg
     4861 gacacgcccc ggtatgaact ggcccacgcc gaggacgcga tgtacttttt gccatacggg
     4921 accatggtgg acgaattcca gcacatcatg tacgccgagc cggacctgac gcccggggag
     4981 cgcaacgcag tatgggcaaa gctggaaagc gaataccgcc cctggctgga cttcgcgggc
     5041 ctgcccttct acggacgcgg cgcgggctgg cagcgccagc tgcacatcta cgaggtgccg
     5101 ttctactata tcgactactg cctggcccaa acggtggcgc tgcagttctt caccgcgttt
     5161 ctgcacgaca aaaaggacgc ctggcggcgg tacctggctc tggtgggcga ggccggcggc
     5221 aagacgtatc cgggtctcgt ggcggcggcc gggctggaca gcccgtttgc tcccggaacg
     5281 ctggcgaagc tgggcaaaga agtgggcgcg tggatcgcgg cgcaggacgc cgcgctgaac
     5341 gcccggtaaa ggacccggct ccggaatgga ccggacggaa aaggcccggg aagggcaggg
     5401 cggaacagga ggcctgaaga tatgatacag gaaaagggaa aacgaagggc ccggcgcgcg
     5461 ccggcgctgc tggaggcgta tcttgcgctt gcgcttttgg cgggctgtgc cggcggcgcg
     5521 ggcagctccg cggcggaaca gaacagtgcg tcctcctccg gtgcggcgga aagcgcgctt
     5581 tccgcccccg ctgcgccgca ggagaccgtt ttctccggtg cgtcctcctc ctgcctgccg
     5641 tccgaaccgc cggaggatgc gccggaaacg ctggggccgg ggcattttac cagcgtacag
     5701 gatcgcgcgg agggggagca ggatccctat ttggagctat acgccgacgg cacgtttatg
     5761 ttcatgtgca atgtgtatca ggggatgacg acgtatctgg gaacgtggag ctatgagagc
     5821 ggcgttctga cgctgacgcg ccccagcgcg gaggagacct atgttttctt cgcgcaggac
     5881 gccgacacgc tgctgatgca cagcggagac ctggtgatga cgcagcccgg cgcagtgctc
     5941 acacggctgg cgcagtagca tagaaagcgg gcgcggtgca aaaccgcgcg caaaaagcat
     6001 aaacaacaaa aaacacgccc tgaagcgaag cggaatgctg cctcagaacg tgttttttct
     6061 tttttggtat tttccctgcg ccgattgttt atgataattt tattctgcgc cgcacccgtt
     6121 tgtgcctgta tgccgcgatc tacttccagc cgaggtcgcg cagaatatcc tgccgcgcgg
     6181 caaggcccat acgcgccaga tgctcgatgc ggtcgtccgt catctgcgcg gcaaggcccg
     6241 agatgctgaa cgccgcctcc gcacggccgt tcatgcccgg caccgccagc gccacacagc
     6301 ggatgcccag ctcgttttcc tcgtcgtcga tggcatagcc cgccatgcgc acgcgcttga
     6361 gctgctcgat gaatgcgtcg aaatccgtga tggtatgcgc tgtcagcgcc cggatgctgc
     6421 ttttgcgcca cacgcgttcg gcctcgccgt agctctgggt ggccaaaatg gccttgccca
     6481 cgcctgtgca gtacatgggg atgcgaagcc ccacatggct ggacatgcgc atgccgccca
     6541 gcccggcgcc ggcctccgcc ttatagatgt atacgatgtc cacgccgtcc tggataacca
     6601 gatgcaccgc ctcgcccgta tggcgggaaa gcttgtccag atgcggcttt gcaacggaca
     6661 tgatatccat atcgttgacg atgccgctgg acagctcgaa catcttcatc gtcagccgat
     6721 agtgcgtgga gaaactgtcc tgcaccgcgt agcccagcga tacaaggctg gagagcagcc
     6781 ggtgcgccgt gcttttgggc aggccgctct cggcgctgat ggtctgcagg gacgcgccct
     6841 tggggtgtgc gcacaggact tcgatcagtg cgaagatgcg tgccacgctc tgcacgccgc
     6901 cgttgctgtt ttccacaaga gcaccccctt ttctttgttg cgttccacaa tgcgaaacgg
     6961 ttttccgtct gtattgtaac ttgttttgcg ccaaagcgca agcccccggc cccgcttgga
     7021 tgcaagaaat atccgctttt gggcaaaaaa cgcaagaatc gcattgacaa taaaacctgt
     7081 ttgtttataa tgaagccaga aagaaagtgg aacggcgttc cacaatatgg aacacacccc
     7141 caccgggaat aaaaggagaa gattgctatg aacgacgtga ttcagaaaat ctacgacatc
     7201 ggcatcgtgc ccgtcatcgc catcgacgac gcagacaagg ctgtgcctct ggcaaaggcc
     7261 ctggtcaaag gcggcctgcc cgcggccgag gtcacgttcc gcaccgcagc ggccgaggag
     7321 gccatcaagc gcatcgtggc cgaagtacct gaaatgctgg tgggcgcggg caccgtgctg
     7381 acgaaggagc aggcggaccg agccatcgcc gccggcgtca agttcatcgt cagcccgggc
     7441 tttaaccccg agatcaccaa atatgtcatc gacaagggca tgctgatgat gcccggcacc
     7501 gctactccgg gcgagatgga gcaggccatg agcatgggcc tggacgttgt gaaattcttc
     7561 ccggccgaac agaacggcgg cgtcgccaag ctgaaagccg tggccggccc ttacacaaac
     7621 ctgcgctgga tgcccacggg cggcgtcaac gccaaaaacc tgctggacta cctgtccttt
     7681 aacaagatcg ttgcctgcgg cggcacctgg atggtgaaga aggacctcat cgaggccgaa
     7741 aactgggacg aaatcgagcg tctgacgcgc gaggccgtca acaccatgct gggcttcgag
     7801 gtcaagcaca tcggcatcaa caccgagaac gccgaggaag ccatgaaggc cgccaagctg
     7861 ttcgagctga tgttcggctt cgacgttgcc gagggcaaca gcagcatctt ctgcggcagc
     7921 cgggccattg agatcatgaa aaagcagtat ctgggaaaga acggccacat cgccatcggc
     7981 accaacacgc tgccccgcgc catcgcctat ttcaagagcc gcggcttcga gttcaacgag
     8041 gagaccgcca gccccaaggc catttacttc aaagacgaga tcgccggttt cgccgtccac
     8101 ctcgtgcaga agtaattttt tacagctttg cacagcaagg ctgtaaaagg cggcctgagc
     8161 tgcggcattt gcggttcaat gtttttataa ttgcagataa gatatataaa agacaggaga
     8221 gtattttttc atggcaaaga aagttgttac cttcggcgag ctcatgatcc gcctgcagcc
     8281 ctataactac gagcgcttcg tccaggccga ccacctggaa ttcactttcg gcggcggtga
     8341 ggccaacgtg gccgtcagcc tggccaacta tggcctgga
//
LOCUS       DAWBSB010000090         8293 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1819_length_8293_cov_3.981913, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000090 DAWBSB010000000
VERSION     DAWBSB010000090.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 8293)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8293)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8293
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1819_length_8293_cov_3.981913"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1534 a         2697 c         2400 g         1662 t
ORIGIN      
        1 gcgaaggctc tttggacatg cccgcaatca tcgacgcggg gttggaaagc ggcagcgaat
       61 attttctcat tgagcaggac gatcgctacg gtcgggacgt gtgggaatcc ctgcgcattt
      121 cccgggacaa tctgatccgg atgggatacg aaagctggct gtaacgcgaa acgacgcgaa
      181 acagggcggg atatgccgaa gcgcgtcccg ccctgttttt tcgcgtttgc gcgcacatat
      241 tccgccccgc gtctgcaaac acttttacca ctgccgtaag atcctatgcc ttcccgggcc
      301 ggaacgcatc caacaaggct ttggggacag tatgaaaacg gccgccgcaa atatgtttcg
      361 cagaaggagc gacagaaaaa tgaaactgga cgcaaaaaaa tacgacgaga tgcgcaaggc
      421 cgcgtcgccg ccctcgccgc ttttgaagga ctgcctgtgg gcgtttttcg tgggcggcgc
      481 catctgcctt ttgggcgagg gcctgcgcac gctgtatgcg gccctgggct tcacacttac
      541 cgacgctggc acactgacga gcgccacgct catcggcctg tcggccttta ccacagcaat
      601 gggctggtac cagaagctgg ccgccaaggc cggcgccggt acactggtac ccatcacagg
      661 ctttgccaac gcggtcgtcg cccccgccat cgaattccgc aacgaggggc ttgtctccgg
      721 cgtgggtgca aaaatgttca tcatcgcagg gcctgtcatc gtttacggca tgctggccag
      781 cgttgtgtgg ggcgtgatct accatttcat ccgcccgctg atgggagggt aacgcatgcg
      841 taagggagac accattctgt ttgaagctcc gcccagcgcc gtcgcatatg cggccgtggg
      901 cggcaaaaaa gaggcggaag gccctctggc agacgccttt gatatgctca tcgccgacac
      961 cctgtgcggc gaaaagacct gggaaaaggc cgagagcgat tttgcccgct actgtctgga
     1021 ggccgcgctg aaaaaaggcc gtctgcaggc ggacgcgctg gacgccgtgt tcgcgggcga
     1081 cctgcagtgc cagtgcaccg cgtcggccta tacgatgcgc gggttcgaca ccccgtacct
     1141 gggcctgtac ggcgcgtgca gcaccatggc cgaaggcttt ggcctggccg cgtgcatggt
     1201 ggcaggcggc cacatgcagt atgccgcggc catgagcagc agccatttct gcgcggcgga
     1261 gcgccagttc cgcacgccgc tggactatgg cggcaaacgc acgcccacgg cccagtggac
     1321 ggtaacaggc gccggtgccg ccatcctggc tccggccggc ggtccgccct atgtgcgcgc
     1381 cgtcacattc ggccgcgtgc gggattataa cgtcaccgac atcaacaata tgggcgcggc
     1441 catggccccg gccgcctgtg aaacgatcct gcgctacttc gccgatacgg gcgaaaaacc
     1501 ggagcacttt gacgccattt tcaccgggga cctgggcttt gtgggccgca cgctgctgct
     1561 gcagttgctc aaggaggaag gtctgccgct ggaaaaccat caggactgcg gcctgctggt
     1621 atacgaccgg gacgcgcaga acgttcaggc gggcgggtcc ggcgcgggct gcagcgccac
     1681 ggttctgtgc tgccacgtgc tgcccgcgct gcgcgagggc cgcatgaagc gcgtgctgtt
     1741 cctctccacc ggcgcgctga tgagccaaac tacgttttta cagggcgaga gcatcccggg
     1801 cgtcgcccat ctgatcgagc tttcttccga attgccggag gaaacggaaa aaggaggcgg
     1861 cgtgcgatga attttgtatg ggcgtttctt gtgggagggc tgatctgcgt ggtaggccag
     1921 ctcctcatcg acctgaccaa gcttaccccc gcgcgcattc tggtgctgta cgttgtggcg
     1981 ggcgtggtgc tcagcgcgct gggctggtac gatccgctgg ttgattttgc aggcgcgggc
     2041 gccacggtgc cgctcatcgg cttcgggcac ctgctggccc agggcgtaat ggattctgtg
     2101 gcgcagagcg gccttgtggg cgcgctgacg ggcggcttta cggccgccgc gggcggtgtg
     2161 acagcctcgt tgacggcggg ctttctggcc gcgctgctgg gcaaaagccg ggaccagagc
     2221 tgaccccgct gcgccgggcg cagaacccgg ccaaggcacg acaaaaaacc cgctgttcag
     2281 acgaacagcg ggtttatttg aaagcgtagc ttacttgccg gctttgtctg cggccttggc
     2341 cagacgggaa accttgcggg aagccgtgtt cttgtgaagc acacctttcg ccgcggcctt
     2401 gtcgatcgcg acgacagcag ccttcacagc agcgtctttg tcggcggcgc cggcggaaat
     2461 ggcggcgtct gccttcttga cgactgtttt caggttggac ttgatcgctt tgttgtgcag
     2521 agcctctttt ttcgcctgca ccacacggtc tttctgcgac ttaatattag gcatcgtacc
     2581 acctccttaa tacccataac agtgcaattt ataataccac tttttgcccc gcaaggcaag
     2641 cctttttttg aaaaaagcgc ccaaacaggc gcattatctc ccgaaaggaa ggaatatcat
     2701 ggcagtatac acggatatcg ccgccgaact gtatcccgaa tcccgcggga gcgtcccccg
     2761 cggcgtcaag accttcacgg ccaccgcgtc gggcatcggc ctgacgcggg tggagattga
     2821 gcgcagcggc ctgcgccgcc ccaaaggacg gtacatcacg ctggatatgc cccagttcgc
     2881 cgccatcgac gaccgcaacg aggcttatgt atgggccatt gccagccagc tgcgcgcgct
     2941 tctgcccaag gaggggcttg tcctggtagc cggagtgggc aaccgggccg tcaccgcgga
     3001 tgcccttggc cccgaaaccg cggaccgcgt gttcgtcaca cgccatctgt gcgaacaccc
     3061 ggacgccggg gacgatgtag cgctgcggcc cgtagccgcg ttttcaccgg gggtagaggg
     3121 caatacaggc cttgccacag ccgagcttct gcaaagcgtg gtgcgcaggc tggagccggc
     3181 ggccgttgtg tgtgtggaca gcctgtgtac ctcggacgtg cgccggctgg gatgcagcgt
     3241 ccagctttcc acggcagggc tttcgcccca cactgcgccg ccgctcacgc agcagacgct
     3301 gggcgtgccc gtggtagccg taggcgtgcc caccgttatg gaggccgaca cgatgctggg
     3361 cgcaaaacgc ccgctggtgg tgacgcccaa ggacatcgac gccatcatcc gccggggcgc
     3421 gacgctgctg gcgctggcca tcaataaagc gctgcagccg gcgctctcgg tgggagagct
     3481 gacgttcctt acctcttaat agatacgtcc ccttttattt gaacgagttt tctatctcct
     3541 ttattctgtt ttctatcaga tatgcgctca tatctctttc ttctgcataa ccccttgttt
     3601 gtcttatctc atctctactg ttaaaactaa agccaaacat tttatctgcc cgttccggat
     3661 ctgatttgaa ccggacctcc tccataaaat gcttcatttc tttgtttttg aacgcaagca
     3721 tgatttcttc ccaccgaatt gctttacttg gtgtatctgg ttcagagggg aattgtgcat
     3781 agagtccaag caaaataaac caatccgcat tatcttgggg cgtgaatcgg ttgaaccaaa
     3841 gcatattttg atatctctta tttgggtgac gataggctct tgacatcttg acctgaatcg
     3901 tattgaccag attcacccca ctacagaatt tatacagtaa caggtcgatt cctttttcct
     3961 gcgccgatgt gggaacaaac actgacgcat tctttatgtt gtctgataaa tactgcgcca
     4021 cagcaaattc gccgtattgt attgtgaact ttgcctgcaa ctttaagcct ccttatacga
     4081 tacacagcga gaggaatgtt tcctcttacc tgttttgtaa gccgtttatt ctataattta
     4141 ccgcatctcg cgcatcctgc ccaaccgctg aagcgcgcaa aaccgccccg gcccagcgca
     4201 ccagcagcgc ataatgctcc ggcgcctcct gcggcgtgaa tgccttttgc ccgcgccccg
     4261 ccagcacggc ccggctgtcc gcgtcgggca gatgccgctc cagcgcggcg gcgtcctcac
     4321 agtacacgcc cgacgcggca aaggcctgcg cctgcaggac aaaaaacgcg gccttgtaca
     4381 gcgcaggcag attggcagag acatcctcga acaaaaagct gtgacacgcc gcgtgatata
     4441 cattcgccgc gcccatgtgc agggcgccac gcgcgtcctg cgggcacaac agcgcgccca
     4501 cctccgaaag tgaaccgtac agcgcctctg tgtcatggta aaactggaac aggtccgccc
     4561 ggggccaagc cagcagctct ttcctgcctg aaacaaagcc gcaggccttt tcgctgtccg
     4621 gcatgctgcg caccaattcc cggtaagccg acaggtcggc cgtgtccagc ctgtccaaaa
     4681 tgagcacaaa atcaatgtcg ctgctctctg tggccgcgcc ccgccggaca ctgccctgca
     4741 gccccataaa ccatacccgc gcaccaaaac gcgccagcgc cttttccgca agcattttgg
     4801 cccacgccct gtgatctgtc ataattccct ccctgtgaca atggtgaatc tctctttcct
     4861 ccgcctgccg cccgtgggcg aatggcggtt caggtcccgg ctgcttttat tttaactgga
     4921 tgtgcatttg ggccggtgcc tcgttccatt tgacgggata ccccgctccg tggctgcaaa
     4981 atacggaatc cggcgtgttt tccacatccc tctccgggtc gtagccggtt tgttcggcca
     5041 ggtgctgctg ctgtgcgggc gtacagggat gatatgcctc gaacgccaac gaaagctgcg
     5101 cccgtccctt gcccagcgcg gtcagctccc gggcataggc ctgcatctgt gccacagggc
     5161 atgtacccgt cacagccacc cggccgttgc ggcctgtcac aggcgcttca taccggccgc
     5221 ccatacgcgg tatgtcggac aggatacgcc ccgccagtgc cgcatccgcc tctgccgtga
     5281 aagaatacca tggctccagc agcacgcttt ccgcctgcat cagcccctgg cgcaccgcac
     5341 ggtatacggc ctggcggaaa tccccgccct cggtgtgctt cagatgggcg cgcccggcca
     5401 gcagcgtcac gcgcacgtct gtcagcggcg cgccggtaag cacgccccgg tggctctttt
     5461 ccagaacgtg ggtgcggatg agattctgcc aattctgtgc cagcacatcg gtatggcatt
     5521 cactgtcaaa cgttatgccg cttcccggcg cgcccggcga aaggcgcagg tgtacctcgg
     5581 cataatggcg cagcggctcg aaatggccgc agcccactac ggcgtttgcg atggtttccc
     5641 tgtacagcac gccgcagggg ccgaacgata cctctgcgcc gtaccgctcc cgcagcacgg
     5701 cctgcagtac ctccagctgg acctctccca tcacctgaac gttcacacgc tgcagctctt
     5761 cgctgaattc cacgcccagc agcggctcct cgtcctccac ctcacgcagc agctccagca
     5821 ggcggcgggg cggaagctcc ggccctgcca atacctgggc ggaaagcagc ggccgcagtg
     5881 cgggcggcat acccggccgg gcatccgccc ccacaacatc cccgggcatc acccggccca
     5941 gaccggcggc ggcgcacagc gtgccaggcc ccgcttcggc cagcggcgcg ctttttccgc
     6001 cgctgtatgc ccgcagcccg ttcaccttgt aatacgcagt cccgcccttt tccgggcagg
     6061 ccacattctc tttggggcgc agacgccctg ccaacacctt caaaaacacg atgcggccgc
     6121 cgtttttctc atgacgcacc ttgtacacac gggcgcggaa aagcgcgtct ccggcctctt
     6181 cctgcggtgc ggcgcagaac gccgccaata cgttcagcaa cgtgtcaacg cctgtgccgt
     6241 tcagagccgc gcccaccact gccggacaaa caagccccgc ccgcaccagt gcggcgccgc
     6301 gctcccgggc ggcctgcgcg tcatagccgc ggacaagata atcctccatc agcgtttcgt
     6361 cccgcgcggc cagctcctcc gccaaggcct ctccaatgtc cgccgcgcag aaatccggcc
     6421 cgcacgccag cacatccgcc cccaggcgtg cgcgtaccgc cgccagcgtt ccggcagcat
     6481 cagcggtggg caggtccgcc ttgttcagaa aaaacaccac gggcacgtcg ttttgccgca
     6541 gcagccgcca gacggtctcc gtatgggcct gtacgccctc cacacagctt accaccaata
     6601 ccgcgcagtc cacggcccag atgctgcgct ccatctcgcc gctgaaatcc gcgtggcccg
     6661 gggtgtcgat taagcggaac ggaagcccgc aggccgtaaa cgccgcttcg tcggcaaata
     6721 ccgtgatgcc gcggctgcgc tcgatgacgt catggtccag acaggtgtcg ccgctgtcca
     6781 cgcgcccggc gcgccgcagc gtgccgcccc ggtacaaaag ctgctccgaa agcgtcgtct
     6841 tgcccgcgtc cacatgagcg aaaacgccca cgacagtttc cataaaaccg cccctttccg
     6901 tggtaaatac atcttattat agccgccggg caccgcgctt tgcaagcatc gcccgccctg
     6961 caaaaaaagc aggcgcgctc cgcacgaagc ggggcgcgcc tgccggtgtt ttccgcgcgc
     7021 ttcaatccag ttccagcgcg ccggtataaa gctggtaata agtccccttc agggccagca
     7081 gctcgtcgtg cgtaccgcgc tcaatgatgc gtccgtggtc cagcaccatg atggcgtcgg
     7141 cgtttttcac agtgctcagc cggtgtgcga tgacaaacac cgtgcggccc gtcatcagcg
     7201 cgtccatgcc ccgttgtaca atggcctcgg tgcgggtgtc gatggagctg gtcgcctcgt
     7261 ccaggatcat cacgggcggg tccgccaccg ccgcacgggc aatggccagc agctgtcttt
     7321 ggccctggga caggcttgcg ccgttgccgc tcagcggcgt atcatagccc tgcggcaggc
     7381 gcgtgatgaa atcgtgcgcc cccgccagct tggccgcggc gatgcactcc tcgtcacagg
     7441 cgtccaggcg cccgtagcga atgttgtcca tcaccgttcc ggtgaacagg ttggtatcct
     7501 gcagcacaac gcccaggctt cggcgcaggt ctgccttttt tattttattg atattgatgc
     7561 cgtcatagcg gatcttgccg tccgcgatat cgtaaaaacg gttgatgaga ttggtgatgg
     7621 tcgtcttgcc cgcgccggtg gagcccacaa aagccacctt ctggccgggc tttgcataca
     7681 gcgtcacatc atgcagcacc gttttgcctt cttcgtaggc gaaatccacg tcaaacagac
     7741 gcacatcgcc ggtcaggcgg gtataggtgg tagttccgtc gccatgcgga tgcttccagg
     7801 cccatacatt tgtgcgcgcc gcgcactcct ccagcgtgcc gtccgccccc tctttcgcgt
     7861 tcaccagcgt cacatagccc tcgtcggctt ccggctgctc gtcgatgagc gcaaaaatgc
     7921 gctgcgtgcc ggccaggccc atgacgatgg aattgatctg attggacacc tggctgacat
     7981 tgccggaaaa ctgcttcgtc atgttcagaa acggcaccac gatgctgatg ctgaacgcca
     8041 ggcctgaaat gctcaggttg ggcgctccgc tcagcagcag aattccgccg gccagcgcca
     8101 ccaccacata cagcacattc ccgatgttgt tcagaatggg catgagcatg ttggcgtagc
     8161 ggttggcttt ttcggcgtcg gaaaaaaggg catcattgat ggcgtcgaag gcgcgcttgg
     8221 cctcctgctc gtggcagaac accttaacga ccttctgacc gttcatcatt tcctcaatgt
     8281 agccttcggt acg
//
LOCUS       DAWBSB010000091         8168 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1837_length_8168_cov_2.584741, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000091 DAWBSB010000000
VERSION     DAWBSB010000091.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 8168)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8168)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8168
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1837_length_8168_cov_2.584741"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1270..1279
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7254..7263
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7604..7613
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1666 a         2510 c         2160 g         1802 t
ORIGIN      
        1 atcgtgagca tggcaaaaac cacaagcagc accgcaatga tcgtcagcag ttttttcctg
       61 atcctgtaaa gtgcaaactc atttttatat tttctgaaaa aagaattctt catcctccac
      121 cgtatccctt tccacattcc cgctttttcc ggcaggccaa tgccggcgcc gcatgccgct
      181 gctgcggcgc acggcactcc tgcctgcaaa atttcactct ttaatagtat atgggtttac
      241 ggtccttgtc aaaagtcctt tcagccagag cgcaaaaacg actctcgcgc cccgcagcgc
      301 cggtattatt tcacacggat ggaaaaacgg cggtcaaaat ttgtcacgtc gttgaaatag
      361 aggttgaacc ggtcctccga cgccggcacg gcgccaaaga gccccttgat cacgtcattc
      421 acagcaaacc ccggcccgac ggaaaccgta tcctttccgt caaacaattc cagttcgccc
      481 tttgcggcga ccaaagagcc tccggcattc gccggaatgc gatatccttc gccgcggata
      541 agtacattgg gcagaatgcc gaactcaaac cggatggtgg aggcggggca gccccaggtc
      601 tgcacgcgga tatcaaagcc gtcgtccacc ggcgtgacgg tggctttcac cttcactgaa
      661 agatgaccgg acgtcctgcg cctggagtga tccatctccc accagtcgtt cgtgccctga
      721 tattcaccga agggctgata atattggccc gtgccctcat attccatctc gtaaccgtcc
      781 ccgcagggac ggatattctc catcaagagg tggcggcagt tgaaaaagct gatgccgccc
      841 ttgatgtaaa aatcgatgcc accctttttc aggaacagaa acgcgggttg acgcgccacc
      901 gcccacaggt tgaggcctcc tcgggaaagg cgcaaaaggc ccgagccctt cagcagccgg
      961 tccacctcaa acagctccgg cccggcgggg gcgggcaggt tttgaaaggc ctccggaaac
     1021 agcatcaggt cctctgccgc aagcggaaaa ggccggccgc agcggataca ggcctcaagc
     1081 tgcgccgtac ctatggcgcg cagcgcctca tcatgcagtg catggccgca caacaggtat
     1141 tggtaagcat atttttccgc gtagggtgcg gtgcccttgt cctgccgacg ggagttctcg
     1201 gtgaagatag aaaaatccgt gtgataaaag acgggcatca tttccagatt gcggcgcaca
     1261 tattccagcn nnnnnnnnng tttttcccag atcctgcgcc aaaatcagca acgcgcggtt
     1321 gttgatctcg ttgtatccgc ccgctgaacg ctcggcatat tccccgtcct cattgcaatc
     1381 gatatgctcg gccagatact gatcgacgcg cgccacatag cgctcgtcgg gcataagcgt
     1441 attgagcagt gccagcgcgg ccgagatgac ccagcggtgg ttcggagtat ggaacccttc
     1501 ctccaccatg ccgtcgccca tgcggcgcaa cacaagaaac agctttttct gaaatgcttt
     1561 ttcctccggt gtctcggcct ttaccagctt ggcgggcttt gccagcgcca gtgtgacaaa
     1621 cgctgtgtcc ggcgcagagt gaaaattgca ggaataatag tccatcgtgc cgtcggggtg
     1681 cagatggttc agaagatgct gcaccaggcc gtcggcgcag gccagtgcgc gggggtcttt
     1741 gtaataccgg ctctgagggc agaaatacag cgtcatcggt tttgctgcgg ccgagccgca
     1801 gttctgcgcc actacgccga aatccttgtt gtagtatccg ccgaaatccg ggtcctgcgg
     1861 gtcggtgatc tggtacttct ccagcatttc aaattctttt tcatattctg caacgctgcg
     1921 ctgcaataaa tccattctca ttaccttctt tatttatatg ttgtgttccg cgtgggcaac
     1981 tcacagcagg ccctgcgcgt cccacaccaa aagctccacc agcatcataa gcgccagatt
     2041 ctggccataa cctgtgggcc gcagaggaac atttttataa tattccagcg tatccgccac
     2101 gatggtaccg taggacacct gccccaccag gccgtcgggc ccgatatgag cccgcaccgc
     2161 ctgtgcggcg cgcagcgccg catctctgca cgacgcaggc agcagccccg cgcgaacacc
     2221 tttcagcagc ccgtaggcga agcccgagga gccggaaacc tcaagataag aatcttcccg
     2281 gtccaccagc gtgtgccaaa gcccatccgg ggattgatag cggcacagag ccgccgcctg
     2341 tgcgcggtaa gcatccagga tcaggtcgct ctcccactcc tctagctcca gcaggcccgg
     2401 cagctcggcc gccgcgatgg tgaaccagca atttccacgc gcccaaaatg cgcctgcata
     2461 attgtccatg cgctcaaacg accaaccgtg gcaccacagc cctgtctttt tatccgagag
     2521 atagtgtatg tgcagcataa actgcttttt gcattctccc gcgtaccgct cgtcgcccgt
     2581 gatggaagcg atcttcccca tgaacagcac cgccatgaat accgtgtccg cccaaagctg
     2641 ctggttgttg tcagagtcga tggtgacatg ctgaaacccg cccatctcgg tttttggcag
     2701 gccgtccagc agccactctg cacaggactc acagtagtca cgatactctt ccctgcctgt
     2761 ttcctcgtac aaaaagcaca gtgtgagcag cggcgccatc gtgttgatgt tgtgtacgat
     2821 gggtctgttc agcttggtat cgaaccactg tgtcagatac tccaaatacc gccgttctcc
     2881 ggtgagcctg tagtatttcc agatgccgta cagcgccact ccctgggacc aatcccagat
     2941 atccatatcg aacaggatgt tgttgtcatt cttttcgtcg ttgatgatat tctccagtat
     3001 ctgcgtgctc acccggtata attccgtgcg gtccatctcc ctgtcacttc ctttctctca
     3061 ttcagcgcgg cagcgtgttg agcgcgcggt ccaggtcctc aatgagcgta tccgcgtctt
     3121 ccagccccac cgaaatgcgc accagtccct gcgggattcc ttcaaactgc aacatctccc
     3181 ggttcgggtt caggcccggc gtgtttacga cgctttcaaa gccgccccag ctcggcccct
     3241 cctcaaaaac atcgagggat tttaccatca cgtggatggc ttcgttgtct gcatccggca
     3301 cgaagctcat caggccgctg taccctgtca tctgtgtttg agccagcgta tgtccttcgt
     3361 ctgtttccag gcccgggtag cacacgctgc gcacgcgggg atgttcctgc agaaaacgcg
     3421 ccaccttatc tgccgaggcg ctgtgccgcg ccatgcgcac ctccaaagta cgcaggctgc
     3481 ggatgagcag ccatgcggca aacgggtcca tgcacgcgcc ccaggaagcg cgctgtccgc
     3541 cgcgaagagc gtccatacgc tcttcgctgc ccagcaccac gccgcccagc acgtcgctgt
     3601 ggccgccgag atatttggtc gcggaatgga tgctgtaatc gattcccagt tccagcggct
     3661 tctggtacaa cggcgttgcc caggtattgt cgatcaccgt taccgcgccg caggcgtgcg
     3721 cctgggctgc gatctcgcgc agcggcagca cccgaaaaat attggacgat ggggattcca
     3781 gataaaacac cctggtgttc ggccgcaggc tgcgctcgat ctcttccggc gtaaagcgct
     3841 ccagatatgt tacctccacg ccatactttt tcatctctgt atccaaaaag ccgatgacgg
     3901 gatggtaggc gctgcgcaga gccagcacat ggccgcccga gtgcagcagt gaggacaatg
     3961 tggctgtgat ggccgccatg ccggaggcga acacacgtgc tgcgggcgca tgctccagcg
     4021 cagccagctt tttttccagg atctcgatgg tgggattgtt gatgcgcgag tagctgtacc
     4081 catgctcctc atgcttgcgg gtgaacagtg tgttttggaa caacggcggc acgatggccc
     4141 ccaggtaacg gtcataatca tcgcccaaat gcgcgcagat atctgccggg cggcatggtt
     4201 ttttcattgt aatcgcctct tgctcccccg cattgccacg ggggaattta ttttcttatt
     4261 tattcttgct ctcgtagatc ttatccacat cgcgcatgtg cgccagcatg atctgtgctt
     4321 tctgcaccag ctgccggggc agctgcgcag aaagaaggaa cgcgtcctca tgcgcatcgt
     4381 ccgccactat ggggaagaat gcccccggct ccccatccat caaacgaagc agctcgtaag
     4441 tattgtctat ttctgcacgc gcaagctcgt tgagcctgct gatgcgcgcg tcgccctcaa
     4501 tgggccattc tgtgctgtat gcgggctgct cgtccggcct ggtgtgatcc agcacgtcct
     4561 ggtagcaggc ggcattgctc agcgtacggg acagacacag cagcacacgc acacgccggg
     4621 ctgtgaccgc cagcttgccc cgcactgcgt cgtcatcggt ttcaatgctt tcaaacagta
     4681 cggcggcgcg ttccaaagcc ggcgtcattt taagtacaga aagcctcaga atgcgcgtgg
     4741 ccgtgaaacc cttcacaaaa tccatgccct ggatattcaa aaggtcacgg gcctgctctt
     4801 cggtcgtcgc ctggaacagg aacggagcgt aatatgctgt ttcctcttcg gtgagtgcgg
     4861 ccggtaccgg gaccagagga cggttgatga cgcgctccgc gatcagcggc atccagataa
     4921 aattgtcaaa atgcaggtcg gacatgatcc gcgccgcgtc atccagtgcg caccatgcat
     4981 ccagcagctt ggaggcctgc gcttcgccca cgatctgtgc cgccacatgc cgcagcacat
     5041 tcatcagctg tgcggtatcc tgaggccgca tctcgcggta tgcgtccaaa atgcgtatat
     5101 actcattgaa ctccgagcgg tggatcacag ccttgatgta ttcgctttca gagccggccg
     5161 ccgcatgcag cttgcgaacg aaatccactg tcatgggcaa cgtctgcatc ggacgcacat
     5221 gctcgatgag gtcgctgagc atatggtata ccggcttttt tccatgcata tcacggcccg
     5281 tcacaagctg gcccggcttc aggctggtcc aagcggcgtc catggaagcc tccggcgcct
     5341 tgtagccgaa gatatcgctt gtgatatcaa tgagtgtttc acgcccctgg gagcgagcgg
     5401 cgtcgtacag gacctgcagg aaaccaacaa tccggtccgc catgctcacc ttgcggcagg
     5461 cctcgggccc gttgggaccg ttgtacaagc ccgtggacca gcacacgccc gcgccgctgt
     5521 cgttggtgat gatctggaaa aagccgatgt ccacctcggc gcacaggcgc tgcatggctt
     5581 cataatacag cccgcgcacc tcttcattgt caatgcaggg ggagaaatac atgtcgcggg
     5641 agcggcgcgg atggtcgcat cgcgggccgc gccaggcggg gtggtccaga tacgcctgtt
     5701 cgggcagata gaacgggtcg atgacgacca cagccgcctg catgccgtat ttttttacaa
     5761 tttcgctgcg cctgcgcaca agctccaggt ttctgcgcgc gtagtctgca tccaaatagg
     5821 gctccagtgc ccttggcacc accagcttga acagacaggt ctgaagcatt ccccagttgg
     5881 ggtaggggtc gcccggatgg gtcgaccact cctcaaagcg cttgggcaca tccattacgt
     5941 acatgtgtgt cgcccctgcc tgcgcggcct gtacggcttc ccgctcaaac gcctgcaggc
     6001 tctggggctc gtaattccgt tcgtggaaaa aatgcagcat atatttcttt tccatgcgta
     6061 tctccctctt tcttccgttg acgcctttgt ttcattttta atgttccggc tgcccggcca
     6121 atacatcctc atagctgccc catgttcccg cgcgctgcag cactgtcaca aagtccagct
     6181 ggttgcagcg gttgggatag gcggaaaaca gcgtcttacc gcggtgatcc acacggctcc
     6241 catcgatcat accgtaggta cggccgtcgc tgacaaggaa ttttgcatcc gtctcgaacg
     6301 caccgtagct tgcgcgcccc acctcataag agtatgttgg gcgcagattt ttgtcctcga
     6361 tacacgcccc gtcgtcgcat tggatgccga tggtatactc cagcccatcc gccttgagct
     6421 gcaccgtcag actgcgtccc atgcgggtgt gagcggtacg cagggaatgg acgattccaa
     6481 gcgcccgggc gcgctgcgcc tctatctcct gcttcgtttc gcctgtttcg ggaatcaaca
     6541 gggaaacata ggaataatac tcatccgcgc cgtagtaccg gcttgtatag caggagagcg
     6601 ccgtctcctg tcggtagttg cggcgctgtg cctccatgga atatgaaacg ccttcaccgg
     6661 caaatgcaat acacagagac tgctgcgcgt gggcgcggag tgcggatttg tagcggtgga
     6721 aatgcatcag cccctcagcc gtctcctcct gcacacggta cacgcccggc tccaccggag
     6781 aaatatgttc cgcgtggtac atcacgcctg tcgtcatttc ctgcgctgcc cggggatgca
     6841 cggtgtccac cactacgaac gcatttgccg ctttgagata aacgatgtgg cggtcggcgt
     6901 ctgcattgtg gaaatcgtca tacagccgcg tcttaatgac ctcagcgcct gcaaactgat
     6961 ggaagaacag cttttccgtg cgcacaggca gataatcgcc gatattttca gcatattcca
     7021 aaaagccgtt ttcccggaac atccgcccgt tgcgcatcac cagacggttg tggtaaaagt
     7081 ctgcacggta atggccgttc gtggtaaagc tgtcccggta gccgccgtcc cgcagcagca
     7141 ccgcaccgcc cgagcacagc gcacagatgg actgctcatc cgcgtgccca tgatgcggct
     7201 tttccgcatg gacggggatg gtattctgta agtaatccct tgtcagtttg ccgnnnnnnn
     7261 nnnatcctca cgccagccgt cgcggaaaac agccttcttt cccaccagct cgtccagcac
     7321 ctcacaactc agaagccccg gttcttccgg ctggatgctg tcgtccgccc atacaaaagc
     7381 attcgtcatg ccccgttcat actgtacgcc gttgtccatg gtacggtttt tcaccatgtt
     7441 gcggaactgg cgctcaatgg catatttcag cacaccgtca ttgtgccgct ttgccatcag
     7501 ctccatcgca cccaaactca ggcacgtgga agtgcccgaa tcgaaccttg tatcgccgaa
     7561 ttccggcagc ccgcccgacg gcagaagcat cgccgtatag taannnnnnn nnngtactga
     7621 gtgtactccg cgatgctgtt cacccacagg cccagatagc ttgtggcgtc ctcgatggac
     7681 caattgccga ggctgtcttc aaacagcata tccgccagct tcagacagcg cgcggcggac
     7741 gcgttttgcg ggaacagcct tgcaaacagc agcagcgcga tggcccgcaa tgtggcgcgg
     7801 ttgtgccgtc cccattcgca gtcgcggaag atgtggttga tctccgcata gcacaaggtc
     7861 tccagcctgt tccattccgc ctgcgtgaat gcgtcgcggc cgatgatgta cagcccgcgc
     7921 agcaccggag taaatgagaa aatcgtttcc accacctgga aatgccaggg gtccgtatca
     7981 ttttcctcca acacctccag cggattttta gagggacctt cgatgggctt cccatcctcc
     8041 tgcttatata taaaaggtaa ggagccatcc tcatagcgct gcaccagctc atcctgcacg
     8101 gagaaaaata ttcccaggca ttcttttacc cgttccagcc attccgcttt tcctgtataa
     8161 tgatacat
//
LOCUS       DAWBSB010000092         8072 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1856_length_8072_cov_2.759511, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000092 DAWBSB010000000
VERSION     DAWBSB010000092.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 8072)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8072)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8072
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1856_length_8072_cov_2.759511"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1110..1119
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6710..6719
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1772 a         2291 c         2564 g         1425 t
ORIGIN      
        1 ccacggaaaa ccgtttacgc tgacaggcga ggacatgctg gcgcgctgca tctgccacga
       61 gacgaaccat ctggacggcg ttaccatcat ggaccttgcc gagttcttct atgaggatca
      121 ggagcacgac gacgaggacg aaagcgcaac ggaggaatga gcattgaaca tcctgtttat
      181 gggcacgccc gatatcgcgg ctgctgcgct ggaggcgatc cttgacgcag ggcatccggt
      241 atgtggtgtc tatacgcggg cggataagcc tgtcggccgc aagcaggtgc tgacagccac
      301 gcctgtcaag cgcacagcga tggcgcacgg catccccgtg tttcagcctg ggacgctgcg
      361 ggacgccgcg caggcggaaa acatccgtgc gcttgccccc gagctgatcg tggtggtggc
      421 ctatggccgc atcctgccgc cggaagtgct tgcgatcccc caatacggct gcatcaatct
      481 gcatgtttca ctgcttccca gataccgggg cgctgcgccc gtgcagtggg ctgttatcaa
      541 cggcgaaaag acgaccggcg tttccattat gtatttaaat gagggcttgg acacaggcga
      601 tatcctgcgg gtctccccgg tggaaattgg cgagaacgag acctccggcg agctgtttga
      661 gcgcatcaca caggagggag cacgcacgct gctggcggcg atggaggata tcgcggcggg
      721 cacggcacag gcgcggccgc agaatgacgc cgaggccacg ctggcgccgc cccttacaaa
      781 agaacaggcg catttcggat ttgacgcgcc cgcccgccgt ttgcacgacc tcatccgcgg
      841 tttgaacccg tggcccgttg catattttga gtacggcgga aaaaaggtga aagtgctgcg
      901 cactttgtgt gacgatgcag ccgcgggcgc gccgggtgag atattgagca caaaaccgct
      961 gaaggtagct tgtgcctccg gcgcgctgac gcttctggaa gtagtgcccg aggggagcag
     1021 acccatggaa ggcagcgcct gggccgccgg ccgccggttt acaaatggtg acaaattgca
     1081 ataaatttcc tgcttttgcc gtatatagan nnnnnnnnng tagacgcccc tttttcattt
     1141 aaaataaccc cgcgccgtgc gcggcgaaca gggcatgccc tgttcgtaag cggctttgcg
     1201 gtcataccgc aaagccggca aattgcaata aatttcctgc ttttgccgta tatagaaaga
     1261 ggcatgcgcg cagcgcacgt gccgcttgag gctgcggcag ggaatcaaag gagcagctat
     1321 ggagaatccc cgccgcgcgg ccgtggccgc gctgataaaa caggaacagg acggttacgc
     1381 aaaccttgtg ttgcgccatg cgctggacgg cttcaccgga acagcgcggg accgggcatt
     1441 tctcagcgcc gtgttctacg gcaccgtaga gcgtatggtg acgctggacc atatcctgca
     1501 gaaattcctg caaaagcctc tggtaaagct ggatgcggcg gtgcgcgcca ttctgcgcag
     1561 cgggctgtat caggcgcgtt atctgaagag cgtgcctgtg cgcgcggcca tcagcgaatc
     1621 cgtcacgctc acgcggcaga tggggaaatc cagcgctgcg gggcttgtca atgcggtgct
     1681 gcgccgcgcc gccgcttacg acctcaccgc cgaacattac tccaacgaga ccgagcgcct
     1741 ttgcgtggag tacagcgttt cgccgcagat cgccgggctg ctgcagacgc gctgcccgga
     1801 ccagtgtgag gatattttga aggccagctt cacaccgccg ctcctgcgtg cgcgtgaaca
     1861 cgctgcaaac ggatattggg gcgctggagg aggaatttgc ccgacagggc atggccaccc
     1921 gacgcggcgc gatcccggac agcctgtatg tggaatgcag gggagacgtt acggcgctga
     1981 agctgttcaa gcaaggcctg ttccatgtgc agggcgaggc gtcccagctt gcctgcgcgg
     2041 cgttgtcgcc aaggcccggt gataccgtgc tggacctgtg cgcggccccc ggcggcaaaa
     2101 gcgccacgct ggcgcagtac atggagaaca aggggacgct ggtttcctgc gacgcggctc
     2161 aaaaccgcct gacgctggtc ggcggcgcgc tggaacggct gggcgtggcc tgcggccgcg
     2221 tgctgcacaa cgatgccgcc gtttacaatg aggcgtttgc gggcgcggac gcagtgctgt
     2281 gcgacgtacc ctgctccggc cttggtgtgc tggcgaaaaa gccggatatc cggcagaaaa
     2341 cgctggacgg cctggatgga ctggtacggc tgcagcgggc gatcctggag acagccgcgc
     2401 gctatgtgag gccgggcggg cggctcgtct actctacctg tacgctgaat ccggatgaaa
     2461 atgccggtat cgtccggccc tttttgaaag agcatccgga gtttcgtacg cgcagtgtgg
     2521 agtgtgtttt gccggggacg acaaaagatg acgattttct tacactttac ccgttccgca
     2581 cgggaacaga cggctttttc atagcatctc tggaaagatt gtgataaaat gaaccagaca
     2641 ggaatctctt catataccga gccgcagctt gcggaactgg tgaaggaaat ggggcagccc
     2701 gcgtttcgcg caaaacagct tttcaaatgg ctgcatgaaa agcaggcgcg ctctttttct
     2761 gcaatgacga atcagccaaa ggcgttcctc gccgcgctgg aagaacgctt tttcatcgag
     2821 acgctggaga ctgagcgccg ccagcaatcg aaggacggta cggtgaaata cctgttccgc
     2881 ctgccggacg gcaattgcat cgaaacggta ttgatgcgct attcctatgg caatactgta
     2941 tgcgtgtcca cacaggtggg ctgccgcatg ggctgccgct tctgcgcttc cacgcagggc
     3001 gggcgtgtgc gcaacctcac ggcgggtgag atcgcaaacg aggtatacgc cgctgcccgg
     3061 gataccggag aacgcgtttc ccatatcgtg ctcatgggca ttggcgaacc gctcgataat
     3121 tttgatcatg tcatggactt cctgtccatc atctcttcgc cgaatggcgt gaacatcggc
     3181 atgcgcaaca tcagcctttc aacctgcggc ctcgtgccga tgatctacaa gctggccgaa
     3241 cggcatcttg gcctgacgct ttcggtctcg ctgcatgcgc ccacggatga aatgcgcagt
     3301 tcgatgatgc cgatcaacga tgtgtatccc gtggaacagc tcatccgggc ctgccgggac
     3361 taccagcagg agaccggccg ccgcgtcagc tttgagtatt caatggtgaa cggcgtgaac
     3421 gacagcccgc agacggcccg caggctggca cagctcatca agggcatggg tgcgcacgtg
     3481 aaccttattc ccatcaatcc cgtggacggc tcgccgtatt ccgctacgga tgccgccaac
     3541 gtcaaacgtt ttcaaacgct cctgacggag ctgggcgtca acgccaccgt gcgccgccgc
     3601 ctgggcaccg acatcagcgc cgcctgcggc cagctgcgcc gcgaggcaaa aggaggaacg
     3661 accgcatgaa gcttgtcgct aaaacagata tcggcagcca gcgcagcgaa aaccaggaca
     3721 gctaccgggg ggggcgccgc ccggacgacg ctgtttgggg tgccgtgtgc gacggtatgg
     3781 gcggtgcgcg cggcggccgc ttggccagca ccatggcggt ggatgcgctg cagcaggcca
     3841 tcgaggcggg tattgacgag gcgcacaccc cgcaggccgg acgcgcactg ctggaaagag
     3901 ccgtggagca ggccaaccgc agcgtctatg aaaaggcgcg gggcacgcct tcgctcagcg
     3961 gcatgggcac cactgttgta tgcgcgctgg tgcgcggagg ccttgcgcag tatgtgcatg
     4021 tgggcgattc ccgcatttat ctgttccgca acaacaagct gcttcagctt acaaaagacc
     4081 attccatggt gcaggagatg gtggaggagg aagctcagaa tcatccgcgc aagaacctca
     4141 tcacccgcgc tttgggcgtg ggacgggatg tggaggcgga tttcggcgaa aaagaagtaa
     4201 gcccccggga catcctgctg ctgtgcagcg acgggctgag caactgcgtt tctgtgccgc
     4261 agatcgagga aacgctggcg cgcacgcctt tttatgaggc ggcggacgcg ctggtgcaga
     4321 aggcgctgga gggcggcggc cttgacaaca ttacggtcct gctgatgcaa gtagaggcag
     4381 tggaggagaa caatggataa tttgatcggg aaaaaactgg acggccgtta ccaactggag
     4441 gagctgatcg gctccggcgg catggccaac gtctacaaag ccaccgacct tttggaaaac
     4501 cgccttgtgg cggtgaagat cctgcgggag gaatgccgcg gcaacgagga tcttgtgcgg
     4561 cggtttaaaa acgaatccaa ggcgatttcg gtactggatc atcccaatat cgtaaaggta
     4621 tacgatgtca gcgtgacgga caagctccaa tttatcgtga tggagtatat cgacggcatc
     4681 acgctgaaag aatatatgga gtaccgcgcc cagcctttga cgtataaaga gacgctgcac
     4741 ttcatcacac aggttctggc ggcgctgcag cacgcccatg agaagggcat cgtgcaccgg
     4801 gacatcaagc cgcagaacat catgctgctg gcggacagca ccatcaaggt gatggacttc
     4861 ggcatcgccc gcttttcccg cagcgagaac cagacgatga ccgataaggc catcggctcg
     4921 gtgcattaca tcagtcccga acaggcgaag ggcgatacca ccgacgcaaa ggcggacatc
     4981 tattccgtgg gcgtgatgat gtacgaaatg ctttcgggaa agctgccgtt tgaatccgac
     5041 agtccggtgt ccgtggccat taaacagatc gcggatacgg ccacgcccct gcgggagctg
     5101 aaccccgcgg tgcccgaggc gctggcggcc atcaccgagc gtgccatggc caaggaaccg
     5161 agggaacgtt atccttccgc tcaggccatg ctggcggaca tcgacgagtt caagcgcaac
     5221 cccagtgtga agtttgaata ccagtacctt acggacacag cgcccacacg gtacatcgac
     5281 aaagtggtga acaaaacggc tacgaaacag tctggaggcc agcagcgtcc ccgaacaggc
     5341 gcgcggcctg ccgcccgcgg cggcacaaaa accaagaagc ggctggcgct gcccattctt
     5401 gcgggaatgg cggcggcatt tctcattggc gcggcgatcc tggtgttcct catctttaaa
     5461 tatgagggca acggtatgtt cagccagagt atggatgtgg acctgcccaa cttcatcggc
     5521 ctgacgcagg cggagatcca ggaaaaatat ccggattcaa aattcatttt tcagacggaa
     5581 gaggaatata atacagagta cgcggctggt gtcgtatgcg accagtaccc gcgtgcgcag
     5641 acggcttccg accctaagcg cgtgaaggaa aacgcgaaga tcacgctcac catcagcaga
     5701 ggcgtggaga tcgtcacgat gccggacctt tccggtatgt cccgcgccga agcgctgaac
     5761 gcctgtaagg aaaaaggcct gatgcctact tttaagactg aaacggtccc caagggacag
     5821 gcgagcggcg tcgtcctgcg cacggacccg gtctccggta cgcaggtgga ggcgaacacg
     5881 gaaaacagca gcgtcacggt ctacatcagc caggaagagg tggacaagag cactacggtg
     5941 ccgaaccttg tgggtgtggc gtctctgagc gaggcacagg acctgatccg gagtgcgaag
     6001 cttgtgctgg gcggttattc tgaggaatac agcgacaccg taccggcggg agccgtcatc
     6061 tcccaaaacc cggtcagcgg cacgcccgcc aaatggaacg accctgtatc gatcgttgtg
     6121 tccaagggcg cgcaggagcg cacgatctat gtgcacatcg ctgtggatga aaccaacgag
     6181 ggcggcactt atacgctgct gctgaacggc ggcaacccac agccctggtc acccacggcg
     6241 ggccaggcgt ccatctatca atggtccgtc acttcgacgg gcacgggcac gatcacgctg
     6301 cagaagccgg acggtacgac ggcggagcag gctgttgatt ttgctccggg cggcagcgga
     6361 gacctggatt tcggcacgtt tggaggcaac tataaaaagc cggacgatcc ttcgtccaca
     6421 ccgccggacc cttcccagtc cgcgccgtcg ggcgagggcg actgagccgt tattgcattc
     6481 aggcgccggg agcgcgtttc gcgcgctccc ggtttaattg attgatcagc gggggaaaaa
     6541 gaacaagtgg catcaagaag gcaggcaaaa aagaaaggca aagcggcaaa aaacgcggaa
     6601 cacggaaacg tacaggcgtc ggcagccgct tccagaaaga aaacgccgca ggaagaaatc
     6661 cagcaggcgg acggcgtgca gcccttcctc gagacaccgg agcacacggn nnnnnnnnnc
     6721 aactgcaaaa gggaaaagca cacaggtgcc ggcaccggaa aacaatgctt tgaccgggga
     6781 gactttgagc atgtccagcg gcgcttcgga aacagaaagc agcggcgatg cggcgcagga
     6841 taacgctggt ggggccgctg cgccgcaaac gaaagaagaa gcgccgcgga aaacagctgc
     6901 aaagccgaat gctccggaaa ttgaagaggg agcggacgta caggccgggg aggattccga
     6961 aggcaaagag aatgccgcgc cggcggaaca ggtccccgtc gcttcggagg atgcggagga
     7021 cgccggaaag acggacgcag atgccggtgg agagttggag ctgccggacg agccggaagg
     7081 gcttccggag ctttccgccg caccggaggc cgcatatcat gcgcagccgg ccgccgagca
     7141 gaaagctgtg ccgcatcaaa aggcacgcaa acagaagaga cggcattacg cactgtttta
     7201 tatttcgctc ttgacactgg cggtggtgat atgctccgcc gtttcgatct actggatgtt
     7261 cagcacttcc gaaaacggcc tgtttgacgt gaaagaagat gtggaactgc cgaacctgat
     7321 cggtacgcgc tggacagacg tgaaagacga cgcggcgtac agcggctttg tactggaaaa
     7381 agaagaagta tttgacgatg aggcgccggt gggggagatc gtggaccaga atccccgtgc
     7441 gccgcgccag gtgaaagagg gatcccgcat tgtggcaaaa gtgagcaagg gcgttgaaat
     7501 ggtgaccgtt ccggaacttg cgggctggaa caaagatacc gcccgggaaa agctgcgtgc
     7561 gctgaacctg acgctgctca tcaagcccga agagaccacg gatgttccgg tggactgcgt
     7621 tgtgcgcaca gaccctgccg cgggcagtgt ggtcaaggca ggcaccacgg tgacgctgta
     7681 tatccgccgt gacagcacag aagtgtctta tgttactgtc cctgcctgca tcggttctac
     7741 gtcggaacag caggcgagcc tccgcctgac acagcgcggc ctggcaatgc gggtcattac
     7801 ggtagaggat gcgtctccgg caggcacggt gatcgcccaa tctccgaacg cgggtgctat
     7861 ggtgatgcgc ggtacggtgg taacggtcac cgtttcatcc ggtccgccgc ccgtggtggc
     7921 accgcctccg gcacctcctg aatcggaatc cgattcggag gaatcgtcga gttccagctc
     7981 gccgccgccg tcatccggtt cggaaacgcc gccgtccagt tcaacgccgc cgtcctccag
     8041 ctcggagaca tcggcatcca gctcaacgga gc
//
LOCUS       DAWBSB010000093         8065 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1860_length_8065_cov_2.800000, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000093 DAWBSB010000000
VERSION     DAWBSB010000093.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 8065)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8065)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8065
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1860_length_8065_cov_2.800000"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    214..223
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1584 a         2354 c         2585 g         1532 t
ORIGIN      
        1 agcgcatgaa gtgcgcgcag ccgcgcaagc ggcgggtttg aggttattat accatatatg
       61 aaagaacaat gcggcgtatt tgcagggtgc ctttaaggta cggcgcgacc ggatggagac
      121 atcggtacca aaagctgttc tgtgaattac tttgctctcg aaccgggagg tgttgcagtt
      181 cgtgttccgt ccggccgtcg gatcggtaag ggannnnnnn nnnattttga aatatgattt
      241 gatcgcaccc tgctattttg gcacagagag cgccgcggcg ttcgacttta agcgcatcgg
      301 cgcagaggat gtcgctgtca cagatggacg cgtggcgttc cgcggcgggc cggacatcat
      361 cgctgccgcc aacctgtgga gccgctgtgc cgagcgcgtg ctcatcctgc ttaagaccta
      421 caaggccgtt acctttgacg agctgtttga cggcgtatcg gccattccct gggaagagct
      481 gctgcccgcg gacgcggagt tcccggtgaa gggcagcagc ctgtccagca cgctctccag
      541 cgttccggct tgtcagagca tcgtgaaaaa ggctgtggtg gagcggctca agcgcgggca
      601 cagggtgcag gttctgcccg aggacggcgc gctgtacaaa atacgctttt ccatccgcaa
      661 aaatagggtg gaggtaatgc tggatacctc cggcgacggc ctgcacaagc gcggttaccg
      721 caaaaacagt atggaggcgc ccatcaagga gacgctggcc gcggccatca ttgacctggg
      781 ccgcatctat cccgacacac tggcgcagga cccgttctgc ggcagcggta cgctgatgat
      841 cgaggccgcg atgaaggcca tgaacatggc ccccggcctg cggcgccgct ttacggcgga
      901 gcactatgcg tttctgcctg ccgccgtctg gaaagagcag cgggagaaag ctctttcgga
      961 gatccggcgg ggcgtggaat ttcaaggcgt aggctttgac gttgaccctg cggcggtggc
     1021 gttggctgcg gccaatgcaa agctggcggg cgtggaaaag cactgcaggt tctttacggc
     1081 ggatgtgaag gatttttccc ctgcccccgg cgcgatggtg ttcaccaatc cgccttatgg
     1141 cgagcgcctg ggagaccttg ccgccgccgc ggagctggag cgcatgctgg gccgtcggct
     1201 ggaggaaaac cccgtgcgca gcgcatacat tattacggcg gacgcggatt ttgagcagca
     1261 tttcggcaaa aaggccaaac gccgccgcaa gctgtacaat ggcatgatcc cctgtcaggt
     1321 atatatgtat ttttgaccgc atgtccccag gggcgcgcgg aaaggagcgt tttgcaatgg
     1381 caaaaaactt ttgtcctatc tgcggcacgg cgcttgtgcc gggcctggac gcatgcccga
     1441 actgcgggcg taggatcgaa cccgatgaac cggaccggat ggcggagccg ccgcaggctc
     1501 aagcgcagga cggcacccgc gatgccgggc tgtccgggca ggagagcgga agcgcgcccg
     1561 cggccgactt gccggagccc ctcgtgcggg aggcgggcac gggtgaacca cggcaggccg
     1621 gacggctgga tgagccgcct gtgacggtcc ccgcggggga aatcccgacg cagcccggac
     1681 cgggtgcgcc cgtgcctccc atccacgggc aggaggaggc gtatccgtat ccgccgcagg
     1741 gcggcagccc tgtgggggct gcgccgcggc ccatctatat gccgccgcgg cctgagggaa
     1801 cacccggtcc gggcgttcca acgcccgctt atgcgcagcg gcctgcctat tatactcccg
     1861 gcatgcaaag ctacggacag ccggagctgc ccgaaccgat atccatgggc tcctatctcg
     1921 gaatgtccct tttgggaatg gtgccggtga tcgggctggt gttcctgctc gtctgggcgc
     1981 tggacagccc gggccgcccc aaccgccgca actttgcgcg gggctttctg gctgcgcgtg
     2041 tcatcgcatg ggctgtgttt tttgtattga cgttcgtgct gtttttgata atggcggtgg
     2101 cgtccccggc tttttactac tgaggcaggg aaagaaacgc aacggaatgg agagagaggc
     2161 ggagaatggt atattgtatg atcctttcgg cggtttgtat tgcgctgggg ctgctgtgcc
     2221 tgcttcgtcc ggcgctggtt tggaaatgga ccgagcagtg gaaatcgtac cgcgccgatg
     2281 agccgtcgga gttatacagg ttcggcatcc gtttcggggg cgcactgttc ctcgttttcg
     2341 gcgttgtcct tccgtttctg ccgctgcttt tgaaatgagc gcggtccggc gcggaatacc
     2401 cggatgcgta caataaaaag cgtcccgcct gcggcaagat gcagacggga cgctttttgt
     2461 ttccgaaaag aaggagagaa agctcagcgt gtccggcggg aaagccgggc tgtttcctga
     2521 ggatgcttac acctcgaagg aatagcgcag agaacttcca cgctgagcat ggatgcctgc
     2581 cagcgccata tgtaccgcgc cgccctgcgc gccgcgcagc cagccgcagg ctgcgcgtgg
     2641 ctttgtgccg tgcgggccgg ggccgctgtc gctcagcgct acgccaaccg gtccggtgca
     2701 ttcgatgggc ggcaggcccg tttccatcag ctccggttcc tcgccggcgg gcaggggcgg
     2761 cacgatctca taggcgatca ccggcagcca gccctcctcc aactgcggca ggcgcggtgt
     2821 ggggccgcgg tgaaggaaag caagttcatt tacataaagc gtgtgctttg cgcctgtaac
     2881 cgggtgtgcg aaaggaaccg cacaggcggc agcgctcact tcgttgacgg cgaattcgcg
     2941 gccgacgggc atccaccgcg gcccggcggc gacctccagt gacaggcttt ttacagcggt
     3001 ctgcatcccg cggggccagg gggcgtgcac acggtggcac acatagcagt cgcccggcag
     3061 gcacagggaa gcgtaagcgg cgcgcacggc gtctacggcg gcgtcgggcc gttcgcgccg
     3121 cacaaaggga acccaggtga agctgctgga tgcgcacggc tccacaggca ggccgttgat
     3181 caggcgcggg gaaaaggaag cctccgggat gggccgttct ccctccaaca tccgctcttc
     3241 caagggcgtg aggcgctcgc cgtccacccc cgcatatttt ttgcagaacg tgcgcaccgc
     3301 gccgctgtcc agaaacagaa gcacatcaag cgtaagcccg gcgtctgaaa catacagcgc
     3361 cgggatgcgg cacaaatgcc cggcccaggt gaactgccag tccacgggca cggcctgcgc
     3421 cggtgcgccc tggggcgccg tccagaaatt ttttccgtaa taaaccgcaa gagaatcctt
     3481 ttcctccata ccagctccct cacacgcctt ccaaattgaa agcgggagtg tattcctcct
     3541 ttgcactgct tttttcctgc atgaagcctt ccgtcaggcc aagctctacc gcatagtcca
     3601 cagcctttcg gtattcatac gtcgaaatgc gccgccccag ggccttgtgc tccttcgcct
     3661 tgtagaacgg cgtgtactgg ctcatcaggc tcgtcaaaaa gcttcccggc ggaagttctt
     3721 tggccataaa gcgaagcacg gcgcgggtat ccgccgcggc gcccggcagt gccagatgcc
     3781 gcaggataac gccgctgcgc atcagtccct gaccgtccag aacaggcggc ccggcctgcg
     3841 cgatcatcgc ctttacggcg gtgcgggcag cctcgaaata gtccggcgcg tttgaatatt
     3901 cggcggaaag ctccggcgag cgatatttga cgtccgccag ccagatgtcc acatagccgc
     3961 gcagggcggc cacggcctcg ggcgtttcat agccgccggt attgtatacc accgggatat
     4021 gcagcccggg ccgggccata tcgagtgcct cgcataccca gggcaggtaa tgccccgccg
     4081 tgacaaggtt gatgttgtgt gcgccttgct cctgaagctc cagaaaaata tccgccaggc
     4141 gccgggtgga tatctcccgc ccgaaatttc cggcgctgat gccgtgattc tggcagtagc
     4201 agcattgcag cgggcagccc gaaaagaaca ccgtgccgct gccctgtgtg ccggagatgc
     4261 agggctcctc ccagaaatgc agcgccgcgc gcgcgatttt caccataccg ccgccgccgc
     4321 aaaggccatg cgtccgcgtg cggtccgccc cgcagcggcg aggacacaac gtacagtggg
     4381 tgggcgtctc catgccttat cctcccccgt cccgattttc tcaagggata gtgtaccata
     4441 ttttctgcat ttgtggtaca atattcctta agaagaaatc ccggaaaggg ggctgttcat
     4501 gcaaaaaatg cttcatacgg cagaagaaaa gtttctggtg cagcttgcaa aggagtatcc
     4561 caccgtggct gcggcctcca gtgagatcat caaccttcag gccatcctgc ggctgccgaa
     4621 gcccacggag cactttatga gcgacctgca cggtgaatcc gacgccttta cgcatatact
     4681 tggaaatgcc tccggtgtga tcaaggaaaa ggtggaccgt gtgctggccg gcgagatgtc
     4741 cgacgccgag cgcgcggagt ttgcaacgct gatctattat cccgcacaga agctggccca
     4801 gctgaagccg cggcagagcg acctgggcgc atggtatcga aagacgctgt accggctgat
     4861 cgacgtgtgc cgcatgatgt cctccaaata cacccgcagt tttgtgcgca agcgcctgcc
     4921 cggggatttt gcatatatcc tggacgagct gctgcacgcc cactttgagg accacgataa
     4981 ggaattttac tacgggcgta ttctggattc catcattgac attggccgtg cggatgactt
     5041 tatcaccgct ctggcgcagc tcatccagcg gctggccgtg taccgcctgc acatcgtggg
     5101 tgatatcttc gaccgcggcc ccaggccgga catcattttg gatgagctga tgcgccacca
     5161 ttccgtggat gtacagtggg gtaaccacga cgtgcagtgg atgggcgcgg cggccggcag
     5221 cacggtgtgc gttgccagcg tcatccatgt gacgctcagc tacaacaatc tggagacgct
     5281 ggaggacggc tacggcatca gcctgaggcc gctttcccaa tatgccgagg aggtgtaccg
     5341 ggacacggac gtttccggct tttggcccaa gctggtagag gagggcgagt ataccccggc
     5401 tgaccttgca cgcaccgcac ggatgcacaa ggccatcgcc gtaatgctgt tcaagctgga
     5461 atgcgcgctg atcggccgca acccggattt cggcatgcag ggccgcgccc tgctggaaca
     5521 ggtggatttc gtgtctcaga ccatcgtgat cgacggcgtg gaataccaca tgaaagactg
     5581 cgattttccc acggtggacc ccgcacgccc cgccgcgctg acgcccggcg agcgcgacgt
     5641 gctggataag ctgtgccaga gcttcatgca gagcgaaaaa ctggcgcggc acgttcgctt
     5701 tctgtatgcc aagggaagcg tgtaccgcat tgaaaacaac aacctgctgt ttcacggagc
     5761 cgtgccgctg gatgaaaacg gggagttcgc ccgggtggaa tacggcgggg agacgttttc
     5821 gggacgtgcg tggatggaca aatgcgaacg catggcgcgc cagggttatt tcgcgcccgt
     5881 gggcagcgat gcgcgccgcc gcgggcggga ttttctatac tatctgtggt gcggcccgct
     5941 gtcgcccatt ttcgggcggg accgcatggc gtcttttgaa catctgttcg tggacggcga
     6001 atttcccgaa cggaaaaacc cttattatgc atacatcgaa aacgaggatg aggctgtggc
     6061 gcgcggcacc gcgcggcgca tttttgcgga gtttgggctg gattttgaaa cgggccatat
     6121 cgtgaacgga cacattccgg tgcgcgcggc cagcggcgag cagcctgtga aggcgggcgg
     6181 aaagctcatt gtcatagacg gcggcttctg taaagcgtac catcagcgca cgggcatcgc
     6241 gggatacacg ctggtttctt catcccgcgg gctttccctg cgcagccacg gcccgtttga
     6301 aagcacccag aaggcgattc tggacaattt ggacatcctg tctaccaagg acgtgttcga
     6361 gcccagcggc aaacgcgtct atgtagaaga tacagacgag ggcgcgcggc tgaaggacca
     6421 gatcgcggac cttaaaaagc tgttggccgc gtacgcggac ggcagcgtga aagagcaggc
     6481 gtgatgcaag gcatgcgtct gccataaaaa cacaggaggc tgaaccccat gcagcaagag
     6541 agacccggcg cgccgctgcc gtttgaagcg tgcgaagatt ttcgggagct gggcggatat
     6601 acaggctgcg gcggaaggca tgtcaaatac ggcgcgttct accgcagccc cgcgcttgcc
     6661 aatattaaaa ctccggcgga ccgcgcccgt ttcgaggcgc tgggcgtgcg taccgtgttc
     6721 gatttccgtt cggaaaaaga gcggacgctc gcgccggacc cggatttccc cggcgtggtg
     6781 aacattccgg ccagcgccat gctggctgag gacggcagcg aggtggattt cgacctggaa
     6841 aagctgcttc gcagtgaaga cggcatccgg atgctgacag aaggcgtgca tgagagctac
     6901 gcacggatgc cctttggcaa cccggcgtac cgggcgctgt tcggcgccat ccgcgccggg
     6961 caaacgccca ttttgttcca ctgcaccgcc ggcaaggacc gcacgggcgt ggcggcggcg
     7021 ctcatcctca aggcgctggg cgtttcccgg gaggatatcg tggaggatta tctattgacc
     7081 aatgcctgcc gcaccacggg gcgggaccag ttccgcggaa tgctggagcg ggcgggactg
     7141 cccgcagcgc aggcgcagca ggtgacggaa atcgccacag gcgtacggcg ggagagcatt
     7201 gaaagtgcgc tggacgccat tgaagaaaaa tatccgtctt ttgaagaata tctgcgggcc
     7261 gagtttgaaa tcggcgcggc agagcttgag gacatccgcg cgcgttacct tgtatgaggc
     7321 cgcgcatcac ccgacagtaa aggagaaaaa actatgtcta ccccacacaa tgcggccgcc
     7381 gcgggcgatt ttgcaaaaac cgtgctcatg cccggagacc cgctgcgcgc aaaattcatt
     7441 gccgagacct ttttggaaag cccggggctt gttacctcgg tgcgcggcat gctgggctac
     7501 accggtacgt ataagggtgt gccggtatcc gtgatgggaa gcggtatggg catgccgtct
     7561 atcggcattt attcctggga gctgttcacc caatatggcg tggaaaacat catccgcatc
     7621 ggttcggcgg gctcgtacag cccgcaggcc aaggtgttcg acgtggtgct ggccaccagc
     7681 gcatacagcg aaagcagtta tgccaaaacg cagaacggct atgacaagga cgtgacctat
     7741 ccctccgagg cgctgtgcgg cgcactgcgc aaaagcgcgg cgcggctggg catgccgctc
     7801 attgagggcg cggtgcattc cagcgacgtg ttttaccgcg acgccgagcc ggattattgg
     7861 gaaaagctgc gggacgaaaa gggctgcctt gccgtagaga tggagagctt tgcgctgttc
     7921 cacaatgcgg ccgccacgaa aaaaaacgcc gcgtgcctgc tcacaatttc cgacagcttt
     7981 gtatcccatg aggagacgac ggccgagcag cgccaaacga gcttcacgaa tatgatggag
     8041 atcgcgctgg gaagtgtatt ggagt
//
LOCUS       DAWBSB010000094         7998 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1877_length_7998_cov_3.307063, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000094 DAWBSB010000000
VERSION     DAWBSB010000094.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 7998)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7998)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7998
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1877_length_7998_cov_3.307063"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1474 a         2701 c         2160 g         1663 t
ORIGIN      
        1 tccagatatt tgtacacatc gtccggaaat gtgtagtccg taagcgacag cgcaggcgag
       61 ggcagcgtgg cgatgttttt cactttcagc agcgcgcagc ccacggcctt cgtatcataa
      121 aaagcacgcg ttttcgcaat gcagttttca acctttgcct tatccatttt cattgtcgtt
      181 ccctccgttt cggccgctca acccgcggac tgagcgattt tccagcagaa ttcatgccgg
      241 ccgccgtcca gcatggcgcc gttttccggc gcctgcacac cggcgggtag ccgcagacgt
      301 gcggtcgttc caaacggcac cccggcggta aacgtcaccg tgtccccgtc ctttttccaa
      361 cctgcaaaaa tagtgccgtg cgggctttcc acactgcacg aaagcgtgtc gatggccaaa
      421 tccagcgcgg gcgccagcag gaagctgcgg tatgcgggaa tgtccccatc ggggcggatg
      481 cccgcaaaat agcggtaaaa caccgccgat acatccgaaa acatgtaatg gttgtgcgag
      541 ccgcccaggt tccagcactc cgccagcgtc gtgcagccgt gatccaccca gtacagatag
      601 ccggggtagt cctcccgcgt gagcatcttg tagatcacgt ccgtgcgccc gtattcgccc
      661 agcatgttca gcacatattt cgagccgaga atgccataat caatgtgcca gccgttttgc
      721 gcgatgcgct ctgccaggcg ctccatcagc cgcgcctcct cctgcggctt cagcagatga
      781 tggaaagcca cacagccgtc cgaggtctgg catgctcccg ccacctcgcc ggtatccgca
      841 tccacaaaat tgcgcagcag cgccgcacgg atctcctcgc tcctggcaag atacgggtcg
      901 tcaaggccca gcacacggct cattttggaa agcatcctgg cgctgcggta atagcaggcg
      961 gtatccgtca gggccacagg cgctttgaac gtgctcatgt tgacagagat tgccgggccg
     1021 tcgaagggtg cgcaccagtc tccgatgccg tagttcacga tgccgtccac agccatgctg
     1081 gacataaaat caaaatggcg gcaaatgaaa ggataatacc gcgccagaat ggctgcgtct
     1141 cccgtctgtt cgtacagcgc ccagggcagc gtcgtcaata ccatcgacca gtccggccca
     1201 ttgccccagt tatagcccca gcccgttgac ggacatacgc agggaatgga accgttgggt
     1261 ctctggcttt ggcaaaggtc gtccagccat ttttccagaa acaggaatgc gtcataattc
     1321 agcaggagct gatcgtgcac ggtacccgcg tctcccgtcc aggcattctt ttcccggtgg
     1381 gggcagtctg tgggcacgcc ctggcagttg gagatgctgg accaatggca taggcgctga
     1441 atcgtcatca gcgtatcgtc cgaacattca aaatgtccgg tgcgctcgaa agacgtgtgc
     1501 atcacaaggc ccatgatgca cggtcccggc agttcttccg gcagcccctc ggcctccaca
     1561 tagcgaaagc cgtgatatac gaactgcggc gtccattcct ccggcccgtc gctctttttg
     1621 atatatttgt ccgtctggaa ttccccggtg cgcacaaagc cgctgatgtg cttctgttcc
     1681 acatgctgtc cgtccccggt caggtcctcg cagtagcgca gcacaatctc gctgcctgcg
     1741 gggccatagg ccgtcaggcg caccttgcct gcaaaattct ggcccaggtc gaagatccag
     1801 cgcccctcct gcgtgcggta tgcgttcacg ggcgcaagct cctgcatcac gcgtataggc
     1861 tgcatctcag ccgcgcgcag cacgccgccg gcgccgcgca ccaactcggc gtcctgccag
     1921 ccgtctgcgc cgtacgtgct ctcgctccat cccggcagct ccagccgcgc gtcgtaccac
     1981 tcgccattgc ggatggaatt gaacgtgatg ggcccgggcg aggttttcca gcctgtatcg
     2041 ctgcagatgc agtcctgtgt accgtcggcg tatgtgatat acagctcaca cagcagcttg
     2101 ggcaccgcac gccagcttgc ctgacggatg ttccacgcgt cctcggtgaa acagttgtac
     2161 cagccgttgc ccagcgccac gcccagcgta gccgggcctg ccgccggcag gtgctccgtt
     2221 acatcataca cgcggtaata cgacagcgcg tcgtatttgg tgaacgcagg ctccagcaga
     2281 ttttcagaaa cctttttccc ctctatgaac gcctcgtggt accccagccc gcagatatac
     2341 agccgcgcgc tgcgcacctc gccccgcagc gggaacgtgc ggcgcagata gggcgcgcct
     2401 gtgtccgttt tgtccatttt cagaaacggc gccgtgatcc agcgtgcgcg ccaggcctcg
     2461 ttcagcttgc ccgtctcgaa aaaggccgga cggctgacgg ccgcttctcc ggcacggttc
     2521 cacgccgtca cacaccaata ataccgggtg cgtggctgca gcggcgggcc ggcataggcc
     2581 acgctcacat tcccatcacc cgcggtctgg ccggaatccc acacgtctcc ctgccccgcc
     2641 tgtgcgcgcc gcgcgtctgt ggacactgtg atgcggtacg ccgtctgggc gtcgccccgc
     2701 gtgtccgagc gcattttcca gccgaagcgt ggcgtacggt ccagccccag cggattttcc
     2761 tgatgattgc aggtgcactg tacgatttta aagcatccca taaaaagcca tccttttctg
     2821 ttgtgggcgc gcggcccttt tttattttcc gtaccggccc agtttgtggg cagtctgata
     2881 catggcgatc acgttttcca cgggcgtgtt gtcctgcaga taatgcgtgg gcgcaaggca
     2941 gtagccgtgg tgcggcatca tcgtctccag cgtctccgcc acgttctgtg ccaccgcttc
     3001 gggcgtcatc acggccagct ttgccgtaga aatgcacccg tggaacgcca gcctgccgcc
     3061 gtatttccgt gcaagacagg cggggtccat gtccgcggct tccggctgca gcgtatcgac
     3121 cgcccggatg cccatttcga tgaaatcctc aaacgcccag ctggagctgc cgcagcagtg
     3181 gatcatcacc ggcttgccgt agctggaggc caggtcgatg agcggctggt ggcgcgggcg
     3241 gatctgggca cggaacatat ccagactgat gagcggcgcg cgctgcgtgc ccaggtcttc
     3301 gcctgtccag ataaaatcaa catagtccct gcatttgtcc agcgcgcgtt cgaacatggc
     3361 aagctcggcc ttcatgcgcc ggtccagcag cgtcatcacg ctttcggtct ccagcgcaag
     3421 gtccatgtaa atgttctcca taccgcgaat catgccggtg ttgttcatca cgtccgaaat
     3481 gccgggatgt ccaaagtaga cggccttgtg tccatacgct ttgcactttt ccagcaggcc
     3541 gtcgtagtcg aaatcatccg ggtccggcac gggccagttt gcgatgatct cgtcgtcggc
     3601 ttccgccagc ggaaaatcac agtagtccca gtagccgccg tcctcgttcg ccacccagcg
     3661 cgtgacggct ccggtgagcg ggtggacatg gcgctccggc cccggcgccg tgaacagagg
     3721 ctttccggta taggcgggca tcaggccctt aaaatccacg cccagcgcat cattcacgct
     3781 gtccttgtcc gccggtacgc ccaaagccgc cgcaacgcgc ttttggatgc ccgggtttgc
     3841 aaagtatccg atgggcacac ggtctgcttt ttcatggttg aatgtacgca gcactcgttc
     3901 cttggaggtc agtgtcccgt tgtagatctg aatcattttc tctcgccctt ttcccgtatg
     3961 attttatgca ccgtaatcag tgacagattt cccgtttttc tgtgaaaatt caatcgcgat
     4021 agtaagggcc gcaacgtctc cgatggaatc gcccagctgc gcggggacaa cgcagcacac
     4081 cgccgcgctc tgcgccagcg cttcccgggc aatcaccgcc tccatgcccg gccggaacag
     4141 cgcctccgcc cttgcaaaaa tgctgccgat gacgatccgt tccgggttga acagatcgat
     4201 gagaatggac aagcccatgc caagatacgc cgcgctttcc ttcacgatgg cctgcgccgt
     4261 cccgtcgccc gcctgcgcgg ccgtgcagat gtcctttgcc gtcacggcct ccgccgcgcg
     4321 cggcgcgggc cagaaccccg cgtccccgcc ttccgcacgc agctgccgca gccgctgcgc
     4381 gcccagccgg cgcaggcctc cgccgctgca gaagccctcg aagctgccgc gcttgccgta
     4441 gccctccggg ccgtccgccg cgagccgcag atgtcccgcc tcgcccgcca taccgttcgt
     4501 gccctcgtag agccgcccgt ccaaaatcag ccccgcgccc agccctgtgc caaacgtgag
     4561 gaacaccata ttccttgttc cgcgccccgc gccgaaccgc cactccgcaa tggcgcaggc
     4621 attcgcgtcg ttctgcaaat atgccgggca cccataccgc tccgacaaca gcgcgcagat
     4681 gggcacctcg tcccacagcg gcaggttcgg cggcgcctgg atcaccccgc gccgcgtgtc
     4741 cagcgggccc ccgcagctca cacccacggc cgccggcgcc gcgccgccgt tgtactcgcg
     4801 cagcagcgcg tctatcgcct cataaaaacg gggcagaaat tcgtccggat gtgcgtcttt
     4861 gcccgaaggg aacgcgatgc gccgcagaat ctttccgtcc gcgtgccctg ccaccaccgc
     4921 gcatttcgtt cctcccatgt cgatgccgat atacagctgt tccattctgc ttttcctcct
     4981 gcggccggga aggcctgctc cgccatcctc catcccccca agccgtgcat tttgcaaatg
     5041 tcacacacgc cctttacgcc ctgcggtact tgcgcgccgc gttcagcatc acggtcaggc
     5101 tgcgcgggtt cacgtccggc tggatgttgt gcgccgcggc aaagatatag cctccgtccc
     5161 ggttcaggat ctcgatggtc tcatgtactg tttcttcgat ctggctctcg ctggcgaacg
     5221 gcagcagctc ctgtgtgtcg atgccgccgt gaaagacgat cttttccccg taggcagctt
     5281 tcagcccgcg cggctgcatg tccgccgctt tgggctggat cgggttcagg atctcaacgc
     5341 cgcagtccac caggtcgtcg atcagcctgt atacgcttcc gcaggaatgg tgcaggtatt
     5401 tcgcctttgt aaaacgcttg gtataggcga tgcgctcctt gaaatacggc tttaccagct
     5461 cccggaacat gtccggtgaa acgaacggtg cgttctgcgt tgcaaaatcg tccccgctgg
     5521 acgtgtagtg gatgtacggc cccactgccg tgtaataccg ctggatgacc ttcttctggt
     5581 attccagcac acgttcaaaa aagcggtgta tccattcctc gtccagcgcc atacggtaca
     5641 gaaaatcctc aaagccgcac atccagcacc ccagctcaaa gatcccgtaa accgggtgct
     5701 ccgcacagat gacatagtcc gtggtctcgt acagctcctt tgcacgcgcc gcgtgcgccg
     5761 cgatctgcct ttcatcaatg gagtccgggt ccgggaaccg gtaggcgttc agctcttcca
     5821 gcgtcgcgcc tttcagcggc gtgttcacgc tctcccagta caacccggta aaccgccggc
     5881 ggatgcccca ttcgtcgatg tactccgttt ccgatatcgt ctgaaaatgt gagtcccgcg
     5941 gggtaaatat ttcaccgatc gagcgcgtgt caatatcaaa atacttcaaa agacgctcgt
     6001 ccaggcgccg cgtttttcca aacggcaggt ccccgccctg ttttttccca aaccctaaaa
     6061 attccagcag cttgttctcg ctctccccct ccatcgaaga cagcgggttg cccccgaaat
     6121 ctatgatcag gttctccggt ttttcatggc gcatcgtggg cgcaaaatct tctctgcgtc
     6181 ccatttcctt caggcctctt tcctgtatgc tccaatggct tccagcaagg cacggatatt
     6241 ttccagcggg aagccgttgt ccgtctccac ataaaaatat gcgccgccgt tcataacgtc
     6301 aaacgccttt gccgccgcat gcacggcgcg cgtcacatcc gcgggcgtgc cgtgcgtcat
     6361 cacattcgcg cggtcgacgt gtatcgcgca ggccagccca agcctgcgca gccgcgcagc
     6421 cagctcctcc atatcatagg ctgtcagctg cggccagacc gcgttcacgc cgatctcccg
     6481 caggtcctcc agaatgggcg ccgcgtaccc gcaggtatgg aaaaacacgg cttttccctg
     6541 ttctctggcg aattggacca aacggcggta gcgcggcttg aaaaacgaac gccagacctc
     6601 cggcgaaaac agcatcgccc gcgccgtgcc gaaatcgtcc ccgaactgca ccgcgtccac
     6661 gcctgccgcg cacatcaggc gcagctcctc cagctcatat tccaccagca tatcggccag
     6721 acggttgatc tcgcgttcat tgtcgtacac gtccatcagc acgtcttcaa acggacgcaa
     6781 tgcatgcatc cgttcaaaaa agccgaacca tccgtctttt gcataccgcg tttcacgcac
     6841 gcgggcgatg cgctttgcgt aagaggcgaa cgccgcatcc tccgccagcg cgtggggcgg
     6901 acagcggaag ccttccagcg cgcgccagtc cgccagcggc tgccggtacg gatggcccgt
     6961 catggcaaaa atgcgcgatt cccacgtaac gccccaggcg tctgttttgt actcatgata
     7021 gcttccgtcc gggccgcagg cctctgccgg cggccgcgga atgggcatgt ccgaaaaatc
     7081 ctcaaaatcg ccgggatggg ctttcaccag ccgccgaaac gcttccccgt gctcgtacag
     7141 gccccgcccg cacggatggt attccagcgg cgccttgtct accggctcaa aggccagcgc
     7201 cgcctcaaca cgctctctgc tcgtcatgct gtctctcctc cgggtctttc cggccctttt
     7261 acgcaaaaca tcccgcgctg tcgaacggtg tgtccagctt ttcgatgatc cccagccaga
     7321 cggcataccg ccgggcatca aaagccgggt agcctgtcac atagtggttc gccccccgcc
     7381 ggccgtcctc cagcgtccac tccatcacga tcaggcgctg tacgccggaa taggtcttca
     7441 ccttgcccac acagacattt tcattggcgg gcgacacagc ctcgcccata cacagcgctt
     7501 cccgcgtctc gccgtcccgc acgatatacc gcacggtgtg cgcttcgttg gaatcgttgc
     7561 acaaaaagat tttatggcac cagttctccg gctcggcaca gaccatgcac accggctgct
     7621 gcacgcggcc gatgtagtaa aacgccagct ttttgtggaa atagtaatcc accaccgcat
     7681 ccgagatctg cggccagcag tccagcatat tccaccatat gatccccgtc ctgcgccatt
     7741 ttttcaggcg cgtctgttca atgaaaaatt tcttcgcctc cgcctggctg atctgcgaca
     7801 gcatggccag ctgttccagg ctttcgcatt ccgtaccaaa catatcccgc acctggtcca
     7861 ccatcagctg gttgcggtcg tacccacggt cccggatgca cagcgtatac tcggtgttgt
     7921 gggcatccca tgcgtcattt tggatgggcc aaaggtcctt ttccgggata aactgccgca
     7981 gagacgacac cgccggac
//
LOCUS       DAWBSB010000095         7862 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1894_length_7862_cov_3.071730, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000095 DAWBSB010000000
VERSION     DAWBSB010000095.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 7862)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7862)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7862
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1894_length_7862_cov_3.071730"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1640 a         2148 c         2641 g         1433 t
ORIGIN      
        1 ggacacggcc tttggctgtg tcccgcgttt tttgttttga acagagcgcg ttcagtccgg
       61 cgcatgaagg ccggacagct ccgcgtaggt gaaagggcgc ggcagcgtgc ggacggcata
      121 gcacaggtct tccatcacgc aggacaaatg ctccggcgca agttccaccg cggtaaaata
      181 gtccgcaacg gcctgggcgg cgcggatgtc cgcaaacagc gccgccgtct gttcgccgga
      241 cggcaggcga acgccaaagg ccgctccatg agaataacac tcgtatacag gggcttccag
      301 gttcattgtt ccgagttcct cctccgtgtt gatatcttct gatataggta tcatcatacg
      361 gagtttcctt tttttgtcaa ttattaaggc gaaatttgac gaatccgctt gcataattaa
      421 ccgtttgcgg ttaaagtgga acggggaggt gcagcagatg aacctggcca aacgcacggc
      481 gggaacgttg aataagctgc tgaatggggc gacccggcgt cccgccctgg agacgatgga
      541 gcgtgtggcg cgggcgttcg gcgtgcctgt gcgctatttt accgatgcgg ctgttttggg
      601 cactgaattc gaggtgggcg catatgtgga ggaacagggg atgtttgcca tcagcgcccg
      661 ggagcgggac atgctggaga tgctgcggga gcttagcacc tatgagcgcg attttttcac
      721 aaaaacactg gagacaatgt atgagatgcg cgcggagcat agggaaggcg gcccgacgcg
      781 ggagctgttc tgctatctgc cgtcttttcc gatggacagc gctgtgtcca ttgggacgat
      841 gacggcgcag cgcatccggg tgcgcgattg tccggccgcc cgtgaggccg attttgccgt
      901 ggcgatgtgg agcgcggcgc tggaacccct gtatccccag ggcgctgtgc tgggggtgcg
      961 gcgggggcgg gcgctccatg gtgagatcgg tgtttttgcg ctgggagaag cgagctatgt
     1021 ccgccgcctg tatcaggcgc ggggcctgtg ccgcctggac acgatcaacc gcagcgtatc
     1081 caacgttacg gtgcgcgcgg gagacgtact gcggtgtatc ggcgttgtca ccggcgtgct
     1141 ccaggcggat acgggcatag agcttccgtg acggatatga taaaatgaac gcacaccggc
     1201 cgcctgcgcg gccggcaaaa cacgggaaag agcggggagg cacggcgtat gatacaacag
     1261 acattgtttg gggaaacaga cacggggcgg ccgctggcca gcaggatgcg cccgcgcacc
     1321 ctggaggaat ttgcgggcca gaggcatctt ctgggcgagg gcaaggtgct gcgccagctc
     1381 atagacggcg accgtgtgcc ctccatggtg ttctgggggc cgcctggcgt gggcaaaacg
     1441 acgcttgcgc gcatcatcgc caacaggacg aaggcaaatt tcattgattt cagcgccgtg
     1501 acaagcggta tcaaagagat caaggaagtg atgcagacgg ccgaggcgaa ccgccgcttc
     1561 ggcgaacaga ccatcctgtt cgtggacgag atccaccgct tcaacaaagc gcagcaggat
     1621 gcgtttctgc cctttgtgga aaagggaagc atcatcctca tcggcgccac cacggaaaac
     1681 ccttcgttcg agatcaactc ggcgcttttg tcccggtgca aggtgtttgt gctgcacggt
     1741 ctttcggccg gggagcttac ggagctgctg cgccgggcgc tgacagaccc gcgcggcttc
     1801 ggcggcaggc gggtggagct ggcagagggc atgctggaga tgatcgccaa ttttgccaac
     1861 ggggacgcgc gcacggcgct gaatacgttg gagatggtgg tgctcaacgg tgacatcctg
     1921 cccgacggaa gcaccgttgt gcccatggaa acgctggagc agtgtatatc gaaaaaatct
     1981 cttttgtatg ataagcacgg cgaggagcac tacaatctta tctcagcgct gcacaaatcc
     2041 atgcgcaaca gcgacccgga cgctgcggta tactggttgg cccggatgct ggaggcgggg
     2101 gaggacccgc tgtatgtggc gcgccgcctg gtgcgctttg ccagcgaaga tgtgggcctg
     2161 gccgacagcc gtgcgctgga gatctgcgtg gcggcatatc aggcgtgcca ctttttgggt
     2221 atgccggagt gtacggtaca tctgacgcat gcggcggtgt acctttccgc cgcacccaaa
     2281 tccaatgcgc tgtacaaagc ttacgagacc gcaaaaaagg acgctctgga aatgctggac
     2341 gagccggtgc cgctggtcat ccgcaacgcg cccacacgtt tgatgaaaga gctgcactac
     2401 ggtgaggggt accagtatgc ccacgacgca caggacaagc tgacagatat gcagtgtctg
     2461 ccggattctt tgcagggccg gcactactat gtgcccacgg agcagggcag cgaagcccgt
     2521 gtggccgagc ggctggcaca gatcgaagca tggcgcgccg cgcaccgcaa gccccgggaa
     2581 aaagaataag aagaatgtaa cgccccgcgc cgtttgtata ccggcgcggg gcgttttttg
     2641 cacacatggc cgccgggcat atcccacagc gtcggtgcat accgtttaac aaaacaccgg
     2701 aaaaggggac gtgtcgcatg gaaccgcagg agacagcgaa gcgggagact gagcctgagg
     2761 aggaagcgcg caggctgcgc ttcaatgagc tttggctgca ggtcattgag cgttccgaac
     2821 agcgcgccgc gcaggctccc tgacgcgacg gccccgcacc ggcagaggga ggggaatgga
     2881 tatgcagagc agggccgcgg gcgatgcgcc gcgcgccgcc atttactgcc gcctgtcgga
     2941 ggaggacgcg gacaaggcag accctgggca ggacagccgc agcatccaga accagaagtc
     3001 catgctggta cggtacgcgg tagagcaagg ctggcagata tacaatattt acagcgacga
     3061 cgattatacg ggcagcgaca gggaacgccc ccagttcaac cgtttgctgg aggatgcgcg
     3121 caggggctgc ttcgacatcg tgctgtgcaa aagccagagc cgcttcaccc gcgagatgga
     3181 gctggtggaa aaatacatcc acggcctgtt cgtggaatgg agcgtacgct ttgtgggcta
     3241 tgccgacaac gccgacaccg caaatcgtgg caacaaaaag gccaggcaga tcaacgggct
     3301 ggtgaacgaa tggtatctgg aggatatgtc ccagaatatc cgcaccgtgc tggacgcaaa
     3361 gcggcggcag ggggagttcg tgggcagctt tgcctcttac ggataccgga aggacccgca
     3421 ggacaaacac cgcctgcttg tggacgagcc ggcggcggcg gtggtgcggc gcatctatgc
     3481 gctgtacctt tccggctggg gcgcttcacg cattgcggca cagctcaacc gggaacaggt
     3541 gccgaatcca agcgcataca aaaagcagaa caacccggct ttcaaccggg gccgctccac
     3601 cgcgctggca gagctttgga gcgattccac ggtgctgggc atcctgaaaa agcaggtgta
     3661 cacaggaacc accgtgcagc atgtcaacga gaaaatcagc tacaaaagca agcgcctgcg
     3721 ccgcctgccg cccgagcgcc gggccgtggt gcagggaacc catgcggcgg tcatcgacac
     3781 ggaaacatgg gaggccgttc agcggaagct ggcggagggg ggagcggagc agcgcaggga
     3841 aggcaggcgg gacgcgctgg cgggaaaatg ccgctgcgcg ctgtgcggcg gaaagctgca
     3901 aagcaaatac ggccgtgcgg gaaaatacct gcggtgccgc acccacgctg tggccccgga
     3961 gcgctgcccg ggtgtgagca tccggcggga cgaggccgcg cagtgtgtgc tggaggcgct
     4021 gcaggactgc atggcgcggt atctggaccg ggaatatctg gcgcggcatc tgcgtccgcc
     4081 ggaggctgcg gcgcacaggc aggaacagct ggcgctgcgc gccgcccggc tggagcaggc
     4141 gctggcacgc cagcgcgcct gccgcaaaac gctgtatgag gaccgcgtgg acggcgctgt
     4201 cacggctgca cagttcgcgg agctgagcgc tgcgtttgag gcggagatcg cccggttggg
     4261 aaaagagctg gcggcggcgg agcgggaacg cggtgcggcg gcggagcagg cggcgcagtg
     4321 cgtgacggcg gagcagctgg tgaaaaaata tgcccgggcc gatgtgctga cggacgagat
     4381 ggccgaatgt atggtggagg ccgtgctggt gggcaggccg gagcgccagc gcaggcccgt
     4441 tccgctggag atacgctgga ggttttgacc cgcgcagcgc ggacgcagcg ggggagagta
     4501 catatgccgt cccgccaggt ggcattcccc aatgacggca gacggcgtca atgacgctcc
     4561 ggccgtaaaa gaggcggata tcggcgtcgc catggggatc acggggacgg acgtgacgaa
     4621 ggaggcttcg ggcgtcgtca tcctggacga taatttcgca accattgtgg ccgccgtgga
     4681 ggagggccgc gtcatctacc agaacatccg ccggttcatc cggttcctgc tcacttccaa
     4741 tttgggcgag gtgctgggaa tgctgttcgg catgctgctg catctgcccg tgacgctgct
     4801 gcccatccag atcctgttgg tgaacctgtt caccgacggc ctgcccgcca tcgcgctggg
     4861 catggagccg ccggcccgcg acattatgaa gcgcaagccg cgcccaaaaa cggagggatt
     4921 gttcgcccac gggctggcgc gcaccatcgt catacgcggc atgatgctgg gcatggcctc
     4981 ctgcggggca tactgggccg ttctagccac cagcggagac ctgatcgttg cccgcagcgc
     5041 gtgctttttg accattgtgt tttcccagat gctgcatatt tttgaatgca ggggagaggg
     5101 gctggacttc cgtggcaacc cggcgctgct ggcggcggca tttacctccg tggccgcaac
     5161 ggtagtcagc gtgtatctgc ccatggtcca aggctttttc ggcacggcgg ccgtgctggg
     5221 cgcggacctt gtgcctgttg ccattgcggt gctggcgggg ccggtcatca tggccgtgct
     5281 gcggcgcgtc aaaaagctgt tcctgtaaaa aagcatcgga gcgcgccgga cgaattcttc
     5341 gtccggcgcg ctttttcgtg tacagagtgt ttttcccgtg tttatttccc tgtttccgtg
     5401 cgaatactaa caaaaacgga acattgcgca tacagtggcg ggagggcgtt tgccgggccg
     5461 cgggcgcaag ggagggaacc actttggaaa aatttatcaa atggagctgc tttatcacgt
     5521 ccggatgcgt ggtgatcgta tttatcttcg cattgtacat cggcacgctt ctgcccgaca
     5581 cctttcttgt gacggcgggc ggggacattg ccattgcggg catgccgttc gtgcgcgcgg
     5641 ccgcggccgc atccacaggc gaggtggcgg gcctttcggg gggctcgtcc tataacgcgg
     5701 atcttaccat cgcgggaatc gtgccggtaa agaccgtgcg cgcgcaggtg gtggagcggc
     5761 gtgtggtaca ggtgtgcggt acgcccttcg gcatcaaaat gttcgccaac ggcgcgatgg
     5821 tagtaggctt cagcgatatt tacacgtcca cggggtatca gaatccggcc aaaacggccg
     5881 gcctgcgcat gggcgacgtt attacctcca tcgcggggag ggacaccaaa acaaatgagg
     5941 atgtggcggc ggcgctgcag ggactggcgg gcgcgcccgc cgaggtggtc tatgtgcggg
     6001 acggcgtgga gaaatccgtg ctgcttaccg ctgtgaagga ttccaccacc gacagttggc
     6061 gcacaggcat gtgggtgcgg gattcttccg caggcatcgg cacgctcact tttgtggaca
     6121 acaccacggg cactttcgcg ggcctgggtc attccatcca cgatgtggat acgggcacca
     6181 cgatctcgct gctcaagggc gaaatcgtgc cggtggagat cacgggcgcg gtggcgggca
     6241 gcgccggcag ccccggcgag ctgaaaggcc gtttccttac cagcgtgccg acggggacca
     6301 tcaccgtgaa cggcgagact ggcgtttacg gcagtgtgaa aagctatatg aaaggcatcg
     6361 aagcagaggt cgctctggcg caggaggtca ataccggaga tgcggaaatc attacgacca
     6421 ttgaaggcca ggagccgaag cgttataccg ccgtcattga aaaaatcgct ctttcgtctg
     6481 acaatcccaa ccgcaacatg gtggtgcgcg tgacagaccc ccgcctgctc accgccacag
     6541 gcggcatcgt gcagggcatg agcggcagcc ccatcctgca ggacggccgg ttggtgggcg
     6601 cggtgacgca tgtgctggta aacgacccca cccggggcta cggcattttt gcggaaaata
     6661 tggtgcagtc tgcagatgcc gcggcccagc gcgaggcggc gtaaaaaaag cgcgaaaaag
     6721 cccgggatgt gcggtgctgc gcgtttttgg gccggaaaca gcggcttttg ccaaaccact
     6781 gtattttgtg tcgaaacaga tttttcttac caaatatgga aattttttca tggaaactgt
     6841 tgcattatat gggaatatat ggtagaattt aggtcgttga ggaaatgagc gggaaaccct
     6901 cgaacctgag agaaataacc atatggagga acgaaaaaat ggaacaagtt aaactgctga
     6961 tgacggaatt atccccggag cttaccaggc tgtgcgccga gacgctggcg gcgcaggaca
     7021 tcgcggtgac agtgtgtgaa aaggacggtg cgaaggcgct ggcggccctt tcggcggaaa
     7081 agccggatgc cgtgctgctg gatgcgttca tgcctggcct ggacgcgctg acggtaaaac
     7141 agcggtacga cgaagcaggc ggcggaaaga ccgtgttttt cgtcaccggc agtttcacgg
     7201 acgatgcgct ggaaagcgaa atgatgaaaa aaggcttttc gttctacttc caaaaaccct
     7261 ttgaccccga ggtgctggcg gcgcgtgccc gcacggcggc gggccgccat gcggctgcac
     7321 agcctgtggt ggacgacgag tgcaccgtga cggagatcct gcaccagatc ggcgtgcctg
     7381 cgcacatcaa gggctaccag ttcctgcggg acgccatcct gatgacaatg gaaaaccagg
     7441 attatatcaa cagcgttaca aagcgcctgt atccggagat cgcaaagcgc aacggcacca
     7501 cggccagccg tgtggagcgg gccatccgcc acgccattga ggtggcgtgg gaccgcggcg
     7561 atgtggacac gctgaacagc tatttcggct atacgatcca caacctgcgg ggcaagccga
     7621 cgaattcgga attcatcgcc atgatagcgg acaagatgcg gctggacaaa aagagccacc
     7681 gcacggcgtg aaaacggttt gacacagccg tttttgaagc acagcgagaa acggcggcaa
     7741 atgcggttat acaagattta cggccgtgag ccaacattca aaacgataaa ctgtttttga
     7801 cagaacctat aaactccttg atttattggg tgtggaccgc cctgtaatga gaggagtttt
     7861 tt
//
LOCUS       DAWBSB010000096         7861 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1895_length_7861_cov_2.984499, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000096 DAWBSB010000000
VERSION     DAWBSB010000096.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 7861)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7861)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7861
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1895_length_7861_cov_2.984499"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    246..255
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2538..2547
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1524 a         2166 c         2735 g         1416 t
ORIGIN      
        1 tttcggaatt gcctgtgagc gaccggaaaa cagagccggt cgcgggggga gcggtaacga
       61 gcttcaggtt caacctttcg gatcatacaa gctatgaaat tatttattgc gcagaggctg
      121 ttaagaaagg ccgattgaag ctccccgcag aacagcagga ttatttcacc tcggcggata
      181 ttggaggctg ctgggatggc tgtgcttatg aagcggtgga tacaggcgaa agtgagctgc
      241 cgacannnnn nnnnngtttt ccggcggttc aaaaccgcgt ggcggagctg ctgtaagtaa
      301 aaaagaacca tgcgggacgg ctgccacggc atgctgttcc gtcgtataaa agcgcgttcc
      361 gtccggtctt atggctttgc ggagcgcgct ttttatcatt ccacataaaa gcggctttct
      421 ttttcggcgc agaatgtgta aactggagaa aagcataaaa tttttcgaaa tactttactg
      481 atttacttga ctaaagcgaa cggctgtatt atactgcttt tatcacggaa aacggagcgg
      541 ccgcagcggc cgcaaacgcc gccgtggacg gcgaaagcgg gagccggctt tgcacgggac
      601 cgcccgcgcc gcagaacaaa gggggcaagc gccaatggag atcgaaacgg gacgtctgcc
      661 gaaagacgaa caggtgccgc tgcttttgcg cggactgttc gagcagcgcg gctaccgccg
      721 ctaccggatg agcaattttg aagcctatga cctgtaccgg gagaataaaa actttctcga
      781 aagcgaaggg atcattacct tcaccgatgc gtcgggccgg ttgatggctc tgaagccgga
      841 cgttaccatg agcatcgtca agcacacgaa gccggacgct gtttccagca agctgtatta
      901 tgtggaaaac gtgttccgtc tggcgccgca gagcggcgaa tgccgggaga tcagccagat
      961 gggcctggag ttcattggcg gccagggcgg ctatgccgag gctgaggccg ttgaattggc
     1021 cgtgcgcagc cttgcggcca ttggtccgca gagtgttctg gacgtgggtc acatgggctt
     1081 catcacgagc ctgttggacg tgctgggcgt cagggaggaa gcccgcgccg ccgcgctgga
     1141 cgcgctgcgg gccaaaaacg cccacacctt gcgggccatc gcggccgaaa gcggctgcac
     1201 ggaggaagag gcgggccagc ttgccgcgct ggcggctttg gccgggccct tccccgaaac
     1261 gctggacgcc gcccgcgcgc tggtggccgc gccgggcatg gcggacgcct gcggcgagct
     1321 gcagagcctg tacgacgcgc tggaggctgt ggatgcggcg gatactctgc ggctggattt
     1381 ctccacgctc aacgacatcg actactacaa cggcgttgtg ttcaagggtt atgtccgggg
     1441 cgtgccccgg gccgtgctgg cgggcgggcg ttacgacaac ctgatgcgcc gcttcggcaa
     1501 gccgcagccc gccgtgggct tcgcgctgta cctgggcggg ctgggccggg cgtttgcgga
     1561 aaaatccgat tacgacgtag acactcttct tttgtatgat gcaggccaaa gcccggcgca
     1621 ggtggcgcgg gcggtacaga gcattttaaa aagcggccgc agcgtaaggg ccgaacagag
     1681 cgcgccggag ggcctgcgtg tgcgccagac gctgcgcctt ggcccggacg gagcggtccc
     1741 cgtgacagga ggtggcggca tatgctgaat atcgccttgc ccaagggacg cttgggcgac
     1801 aaggtatacg ggctgttttc ctccattggg tacgactgcg cggccattta cgaggacaac
     1861 cggaagctgg tgtttgaaaa cccggagcgc ggcgtgcggt acctgctggt gaagcccagc
     1921 gacgtggcca tctatgtgga gcacggagcg gcggacatcg gcgtggtggg caaggacgtg
     1981 ctgctggaaa cacagcccga tgtatatgag ctgcttgatt tgcagctggg ccgctgccgt
     2041 ctggctgtgg cagcgaaaaa cggctggcag gaggaccttt cgctgccatt gcgcgtggcg
     2101 acgaagtacc cccgcgtggc aaaagagtat tacgaggcgc agggccgcca gatcgagatc
     2161 atccggctga acggcagcat cgagcttgcg cccattctgg gcctttcgga tgtgatcgtg
     2221 gacatcgtgg aaacaggcac gacgctgaag gagaacgatc tgggcgtgtt cgcggacgtg
     2281 gcccaaagca gcgcgcggct cattgcaaac aaggcgagct atcagttcag cgccggcgtc
     2341 atcgacggca tctgcgggaa gttgagggaa aagctatgat tcgtgtgttc aaaagcgggg
     2401 aagtggcccg cgcggatatt ttataccggg gagaatccgg cagcacgcag gccgtggagg
     2461 acgcggtggc ggatattctg gccgatgtga agaaaaacgg agacgcggcg gtgctgcgct
     2521 attgcgaaaa attcgacnnn nnnnnnnccg gacgctgacg atggccgctg gcaacatcga
     2581 ggccttccac aaacggcaga tccgccggaa ttttgtcatc aacgacacgc ccggcgtggt
     2641 gctgggccag aaggtgacgc ccattgagcg ggtgggcctg tatgtgcccg gcggcacggc
     2701 cagctacccc tccagcgttc tgatgaacgc tattccggcg cgtctggcgg gcgtttcgga
     2761 gatcataatg gtaacgcctc ccgcaaagga cggcagtgtg gcgccggcca ttcttacggc
     2821 cgcaagggtg gcgggcgtga cggccattta caaaatgggc ggcgcgcagg ccgtggcggc
     2881 gctggcctac gggaccgaaa gcgtgccgaa ggtggataag atcgtcggcc cgggaaacat
     2941 ttttgtggcc gccgccaagc gccgggtata cggcatcgtg gacatcgaca tgatcgcagg
     3001 ccccagcgag atactggtgc tggccgacgc tgcggcgaac cccgcctatg tggcagcgga
     3061 cctgctgagc caggccgagc acgacaggct ggccaccgcg gtgctggtgt gcgacagcga
     3121 ggcgctggcc ggggcggttt cggcggagct ggagcggcag attcccttgc tgccccgggc
     3181 ggacatcgcc cgcgcgtcca tcgacaacaa cggcaagatc atcattgcac gggacatggc
     3241 cgagggcgtg gacatcgcca acgagatcgc gcccgagcat ctggaggtct gcgtggacga
     3301 cccgttcagc cttttgaaca gcatccgcaa cgcgggcagc atctttttgg gaaaaaacgt
     3361 tcccgaggcg ctgggcgatt atttcgcagg gccgaaccac acgctgccca cgctggggac
     3421 ggcgcgcttt tccagcccgc tttccgtgga tgatttcgtg aaaaaatcca gttttattta
     3481 ttacacgaaa gaggcgctgg gtgcggtgca ggagcgcatc gtcgattttg ccgagcgcga
     3541 ggggctttcc gctcacgcgc gcagcgtgtc catccggttc gaggacgggc agtaagacga
     3601 agcaaagggg aaaagcgaat ggagtttatt gcgttcatcg tgacggtgct tgtgggcttt
     3661 gtggcggaaa gcctgctggg gaattggctg aactggcccg gtgtgggcgc agttttcgcc
     3721 gctgcggcag cggcggcgtt cattcttcgg gcgctgcggc gtaaagaagc gcctgccgaa
     3781 acacaaagcg atgcccaatc cccgggcggt tgccccggcg ggcaggaaaa atgatttttg
     3841 gtgcagggag gcagagaaac gcatgagccg ttttttggcg ggcgcgctgc gcgccgttac
     3901 gccctatacg cccggggagc agccgcgggg cgtggaaacc cttatcaaac tgaacacgaa
     3961 tgaaaacccg tacccgccca gcccgaaggt actggaggcg ctgaacggtc gcgcggcgga
     4021 aaacctgcgc ctgtattcgg accccgcgtg cgcggatttt ctggccgcgc tggcagagac
     4081 gttcggcgtg ggccgggacc aggtgttcgc gggcaacggc tcggacgagg ttctggcgtt
     4141 tgcattcctc gcgttttgcg aaaagggcgc ggcattcgca gacatcacct acggctttta
     4201 cccggtgttc gcgcagctgt tcggcatcgc ggcgcaggag gtgccgctcc gggaggattt
     4261 ttcggtgaat acaggcgatt atgcgggtgt gccgggcacg gtgttcctgg caaaccccaa
     4321 cgcgcccacg ggcctgtgcc tgccgctggc ggagatcgag gcgctccttc ggcaggaccc
     4381 ggaccgcctg gtggtggtgg acgaggccta tgtggatttc ggcggagaga gtgccgcctg
     4441 cctgctgggc cggtacgaca atctgctggt ggtgggcacc ttttccaaaa gccgaagcct
     4501 cgcgggcgcg cggctgggtt atgccgtggg cagcccggcg ctcatcgcgg acctgaacac
     4561 cgtcaaattc agcttcaacc cctataatat caaccgcctg acgcttctgg cgggcgaggc
     4621 cgcgctggcc gacgcaccct acttcgcgcg ctgctgcacg caggtagccg ccgtgcggga
     4681 cgagacggcg caggcccttc gcgcgttggg cttcacagtg acagacagca aaacaaactt
     4741 tctgttcgcg ggcgcgcatc cggctgtttc cggaggagaa tattatgcag cgctgcggca
     4801 aagcagcatt cttgtgcggc actttacgct gccgcgtatc gagaactggg tgcgtatcac
     4861 cgtcggcacc gccgcgcaga tgcagcgcct tgtggatgtg acaaagcaga tactggacgc
     4921 gcggcacagc gccgcgcgct gacaaaaaac acacgaaaca ggagggtttg ccatgagcat
     4981 gcgcatcgta aaatccgcgc gccgcaccga cgaaacggag atcagcctga cgctgaatct
     5041 ggacggttca ggcatttacg acgtggacac gggctgcggt tttttgaacc atatgctgga
     5101 gctgttcagc cgccacggcc ggttcgacct cacgctcgcc tgccgcggcg acgtgcaggt
     5161 ggattatcac cataccgtgg aggatatcgc catcgtgctg ggccgcgcgt tcagcgaggc
     5221 gctgggtgat cgcgccggca tcaaccgcta cggcacctgt acgctgccca tggatgaggc
     5281 gctcatcctt gcggctgtgg acatcagcgg gcggccggcc gtgggctatg cgctggaggt
     5341 ccccaccgaa aaggtgggcg atttcgacac cgagctggtg gaggaattcc tgctgggctt
     5401 cgcgcgcagc ctgggctgcg cggtgcatgt gcgccgcctg gcgggcacaa acagccacca
     5461 tatcatcgag ggcatgttta aggcgctggc ccgcgcgctg aaagaggccg tcgccatcga
     5521 tgaaaaatac gcgctggaga tcccatccac caaggggacg attttgtgaa tgcggaggga
     5581 cggatatgat cgcggtgatc gattacggcg ttggcaacct gttcagcctt tcgtcctctc
     5641 tggcgtatct ggggctggaa aataaaatca cgcgcagcgc gcgggagctg gacgaggcgt
     5701 cgcatatcat cctgcccggg gtcggcgcgt tcggcgacgc gatggaaaag ctcgcgtcca
     5761 cgggccttgt gcccgagctt gagcggcagg cggaaaacgg caagccgctg ctgggaatct
     5821 gccttggcat gcagcttctg tttgaaaaaa gctatgagta cggcgagcac gcgggccttg
     5881 gcatgctgcc gggcagcgtc tgcccgctgg aaccggatct cgcggcggcg ggctttagtt
     5941 acaaggtgcc gcacatcggc tggaacagcc tgcatctggt aaggccggaa aacccgctga
     6001 tggccaatac gcgcgagggc gatttcgtct atttcgtcca ttcgttctat gtcaagggct
     6061 gcggggacgc gctggtggcc ggcgccgaat acggcgtgac ggtgcccggc gcggtggcgc
     6121 ggggcaatgt gtacggctgc cagtttcacc cggaaaaaag cggagaagcc ggcctgcgca
     6181 tcctgcgggc ttttgcggcg ctgtgacggg aaaaatacag aaaccggccc gcgggcggcg
     6241 tgcgcgccgc cggggcgcag aaaggaagaa gcggccatgc agatattccc ggcgattgac
     6301 ctgaaggaca aacaggtggt gcgtctgacg cagggagact atggaaaagt ggaggtgtac
     6361 gggcaggacc cggccgccgc cgcgcgcggc tttgcggcgc agggggcgaa gtacctgcac
     6421 gttgtggact tggacggcgc gaaggacggc gcgctctcca acttcgacgc ggtgtccgcc
     6481 attatcgcgg ccacaggcat gtttgtggag gtgggcggcg gcattcgcga cgaggcgcgg
     6541 gtgcgcgcgt atctggagca gggcgcggga cgtgtgatcc tgggcaccgc cgcactgcgc
     6601 gattttgcgt ttttgcagcg catggtggac aaatacggag aaaaaatcgc cgtgggcgtg
     6661 gacgcgaagg acggttttgt ggccaccgat ggctggctga acgtgagccg cacgcagggc
     6721 gtggactttt gcaggcgggt atatgaagcg ggcgtgcgga acgtgatata caccgatatc
     6781 gcccgggacg gcgcgatgca gggcacgaac ctgtccgtgt atgagacgct ggcgcgggag
     6841 gtgccgggcc tcggcgtcac ggcgtcgggc ggcatttcgt cactgagcga gatccgcgcg
     6901 ctggccgcaa tggggacaaa ggcggctatt gtggggaaag ccgtatatac gggcgcgctg
     6961 gccctgccgg atgtgctggc cgcggcgggg gaggaatagt tctatggtga caaagcgtat
     7021 catcccgtgc ctggacatcc gaaacgggcg cgtggtaaag ggcgtgaatt tcaagggcgt
     7081 gcaggacgtt gaggaccccg tggcgcttgc gcggcgctac aacgacgccg gcgccgacga
     7141 gctggtgttt tacgacatca cggccagtgt ggaggggcgc ggtctgttca cagacgtgct
     7201 gcgggatgtg gcgcggcaaa tattcatccc cctcaccgtg ggcggggcaa tcaatacgct
     7261 ggaggatttc gaccgggtgc tcaaggcggg cgcggacaag gtgagcgtca attccggggc
     7321 catcaaagac ccggccctca tcggagcggc tgcgaagaaa tacggcgacc agtgtgtggt
     7381 gctctccatg gacgtggccc gcgtggacgg caaataccac ctgttcacaa aaggcgggcg
     7441 cgaggatacc ggtctggacg cactggaatg ggctgtgcgc ggcgaagccg ggggcgcggg
     7501 ggagatcgtg ctgaacagca tcgataccga cggcgtgaag cgggggtttg acctggagat
     7561 gctggaagcc ctgtcgaagc gtgtgtcgct gcccatcatc gcatcgggcg gcgcgggcaa
     7621 aatggaggat ttcaaaacgc tgttcacgct accgggcgtg gacgcaggcc tcgcggccag
     7681 catttttcat ttcggcgagg tggatatcgg aaatttgaag cggtatctga ctgaaaacgg
     7741 cgtgccggtg cggctgtgaa tgcggagagg aaagcgtgcc ggaatgaaaa aacggggaac
     7801 cccgattttt aaagacaact tgacgccgca gcagcgcgaa cggcagcaag ccgtttttgc
     7861 a
//
LOCUS       DAWBSB010000097         7817 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1903_length_7817_cov_3.022803, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000097 DAWBSB010000000
VERSION     DAWBSB010000097.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 7817)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7817)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7817
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1903_length_7817_cov_3.022803"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1585 a         2391 c         2296 g         1545 t
ORIGIN      
        1 accgcggctg cacattgcac ttcggccgct tatggcaggc cgctgctgcc gcggcggacg
       61 gttcacattt tactgtgcgc cggcctttct ctccgctttg tccgcataaa ccgcccgaag
      121 ccgccatata tatgcaacaa acacaaatag ggagttgttg cggcatgaag ggcaatccgg
      181 aggagcgggc ggtgcaattg ggcgaataca tcacggagac cggcgccacc gtgcgcagcg
      241 cggccaaagc gttcggcatc agcaaatcta cagtgcacaa ggatattaca taccgtttgg
      301 aaaagcagaa tcacactctg tacgacgagg tgcagaaggt gctgcgcgtg aataaagagg
      361 agcgtcatct gcgcggcggc gcagccacac tgcagaaata ccatccggaa atagaataac
      421 cggcaataca gcggcaggcc gtcggcaggg aacgggctgc cgctctgttt tgccttttcc
      481 accgcatgca agccttcaag caacgtcccc acggcagaca gggccgacct gtgttttgta
      541 aaaagcgata aaattctcaa atttattccc ctatttttcc gttttaattt caacaaatac
      601 taatatatac tggaaataac aacaaataca ggcgattctg ttcagccaca tgttttgttc
      661 acccatccct gcggcccacc gcaaggcgct cgtgccgggc tgccaagaaa ggagcggcta
      721 tggaaaataa atttcttgca cacatcgcgg aggacggacg agaacaaacg gttttcgagc
      781 atctggcggc ggagttttcc cgccctttcg gcggcgaggc acaggggctg ctggccggga
      841 cggcccatga cattggaaaa tactctgctg cgtttcagcg gcggctgacg ggcgattcca
      901 ggcgggtgga tcacgccact gccggcgctt ttgaatgtat ggggcgcggc cagcccttcg
      961 ccgccttcgc cgtggcaggc catcacggcg gccttccgga tggcggcggg cgcggagacg
     1021 gcccggaagc cgccaccttt tggggacgga tcaaccgggc gggccgcggc gggctggagc
     1081 cttacggcgc ctgggcccgg gaggtatccc tgcccggcgg acaacctccc ccttcgcaaa
     1141 gcagaacccg ggagcgggca tgttctttac ctgcatgctt tattcctgcc tggtggacgc
     1201 ggattttctg gataccgagg ccttcatgtc cgggaagccg cgcgggagtt gttcaggctg
     1261 ctggaaaaga aggcgccttc catccggcca ctctgatgta ccccgcccac cgtcaaagcc
     1321 gacggaaagg gattcgccgc atgctgaaag ctgccgctcc cttcccgggg agcgtggatt
     1381 gaaaccgcgc tgctggcaat caaggaggct gcgccggcgg tcgctccccc tcacggaggg
     1441 gattcgagtt taaaggccgg agctccgatt gcattttcaa tcagaactcc ggcctttttc
     1501 ttttatcacc ggctgcagca gcctgtaaag cggctacgcg ccgaacgcct tggccacgcc
     1561 cttcagcttt tcacggatgg gcagctcgta agggcagcgc gcctcgcact taccgcagcc
     1621 gatgcagtcc cccgcccttg ccgtctgaga ctcgtagcgc gatctggccc agtccgccag
     1681 atcgtaatgc ttgagatagt tttccagcgt gaagataccc gggatatcga tgcccaccgt
     1741 acacggcgca cagtatccgc acctgcggca gaaattgccc gaaagcccgg cgcggatggc
     1801 ttcgcatgcg gccagctcct ccggtgtaag cgggccgtcc tgctccgccg cagctgtgtt
     1861 ctgctctatc tcttccaacg tggccattcc ggggatggcc agcgtacagg tcttgttttg
     1921 caggatgtac cgcatggcca gcgtaccgtc ggtgatgttg ccgcccgcca gcggcttcat
     1981 cgcaatgacc cccacgttct gcgcggccgc acgctccatc aacgcttcac cctggctttc
     2041 aacgatatta tatgggaaca tgatggtctc gatctccgga taggagagca gccgctcgaa
     2101 gctgtccacc gagtgagctg ttgcgccgag atgtccgacc ttgcccgccc ggatggcgtc
     2161 ctgaatggca gccacggcgc cctcagcaga aaacgccagc tcaaaatcag catcagactt
     2221 tatattgtgc agctgataca ggtcgatata atccgtcctc aggttgctca ggctgatgcc
     2281 gatgtcccgc gccatgtccg cgtaaccgcg ggacatggat ttcgttgcaa gaatgaactt
     2341 ctcccgcaga ccgccggaca atgcctcgcc cagatagctc tccgacacag tgtagccgcg
     2401 cgcggtgtcg atgtagttca cacccttttc cacaaggcgt tccatcagtg cacgggttcc
     2461 ccgtgcgtcg atgcgttgga tgggaatgcc cccaaaaccc acagcagata ccttcaaacc
     2521 tgtctttccc aaaattctgt attgcataac gcagtttcct ccttttgccg cttgccgcag
     2581 gcgcggcgtt gcctgtcaat cccgattata cacccgcgcc ggtttgttgc aaagttcttt
     2641 ttgtgatatg atagagcaaa acgttgagaa tgaggcgcgg ccggaagcgg cccgccggag
     2701 gggagcatgc catgctgaaa gaaaaaacga accgctggtt cctggcattg ggcgccgcgc
     2761 tgctgtgtgc ggggctgggc gccgcggcac tctgggatct ggagatcgac ctcgcgctgt
     2821 attcacccgg gttcctgccc gccgtattga tggaggcctt cggctggttt cctcagtatt
     2881 tgcccgccgt cgtgctgtgc gtctgtattg ccctggacgg tgcgcgaagc atgccgctgc
     2941 gcgccgcggg cggcctgctg gcggttgccg gcagcggcat cctgctgtat atgggcgcgc
     3001 accatcttgt aaagcgcggg atgtccggcc cttccatcac actgtggacc gtcctgctgt
     3061 gcggcctttc gctgggtata tgcgcactgg cgctgtaccg ctcccgcagc ggggggcgca
     3121 aaaagctgga attcgtatgc ctttggggca cggtgtatct gctggccggg cttgccgcca
     3181 tcaacattat caaggctgtc tggcagcgca cgcgctttga cgacatgctc accgccgcgg
     3241 gcggcggctt cgagggcgcg ttcgcacagt ttacaagctg gatgcagccg ttcggcaacg
     3301 gcggttccag ctttccgtcc ggccacacgg ctgccgcgtg cagtgtgttc atcctgacac
     3361 tggcctgtga cgtctgcctc aagtggaacc gccgccgcac gctggtatgg gcgctttgct
     3421 gggcctatgt ggcctttatg gccttgtgca ggctcgtcat cgggcggcat tacctcagcg
     3481 acacgctggc cgcggccttt gtgatgctgc tgctctacct gggcatgcgc aaaacaaaat
     3541 ggtaccgcgc aggcgcacgt gagctgacgg ctcccgacac ataagtatgg catgccattc
     3601 cgcaaaagcc gtgaaaaggc cgttcccacg ggaacggcct tttcattata tttgcagctc
     3661 acgcacctgc aaagcaaaat aatattggat aaagccccgg ccggaccatt ccgcccgtac
     3721 tttataaaaa tagaggcccg gcgtggtggg tgtgctgaaa cggccgccgc ccgcatccga
     3781 aagctccaga tattccgtcg gcttatcgct gacggcacgc atcacccgca gctccgaagg
     3841 gtccttggtg aacagaatgg gcatctgtgc gcccggcatc acgtccacag gctgcagcgg
     3901 cacgtcctcc ggcgagagca gcggcgtttt ttcctgtgtc aggaaaaaat tccattccca
     3961 acccgcccgg cgcacctcct tgtccggaac aagctcctca tccccataca gaactgtaat
     4021 gatgggcagc gcggattccg ggtcctgcgt atcgagggat tccttaataa acgccgtcat
     4081 gccggtggcg atggccaaca gcacagcgcc gatgatgagc ttgcggaaaa aaatctttgg
     4141 cagtttcata cgtttcctcc cggcggggca agcctgtcgt ttttcttatt gtagcatgcc
     4201 ggcgtgtaaa aatccacccg cataaaacaa aagcggcacg catatgggtt atagcaccgg
     4261 gctgcgcccg cttgcccgcg gcagttttcc gtcccggcgc tcccgcgcgg cacggcgagc
     4321 ggcacaaaag ggcctgccgt cattttcaac tgggaggaat cattttatgg agcattcact
     4381 gtatcctgtg ccgccgcgct tcactgtgcg gcggctggta ttgatggacc ttgcggctat
     4441 ttgcacgctg tatggcgctt gcgggcgcga caccgcctgg ggcacacgcg aaacgcttgc
     4501 cgcgctgttt ggcgaaggcg aatggtgggg cggttttctg ggcggagagt tggttgtctg
     4561 ctgcgcctgt gtacccgccc gttccgccac gccccaggct gccgctctgc gcggcgcgct
     4621 ggcgcccgcg cgccttccgg cccgttttct gctgccgccc gccgccacgc ccgagggccg
     4681 ggcctttgcc gggcgctttc tttccctgct gggcgaacgc ctctggccgc cctgtgcctc
     4741 ccccgaaaaa aggctcgccg ccgcagtccc cgtcaaaacg ggcggcctgc tgctggacgg
     4801 ctttttcgag gcgggcttta cgctgttcct cgtgcgcccg ctggcagcgc tgcgcccgca
     4861 ttacctgctg acgccgcccg gccttcggca gcctgccccg ggaacgccgg atgaagacgc
     4921 cgtacggcgt gtgcacatcc ctctggcgga cacgctggcg gtctcccgcc tgctggaaca
     4981 gggcttctgc gggaccgccc tgtacgacgg cgcggacggg cagcccgcgc tccggctgga
     5041 acgaccgaac cccgcataag aagaacgcgc ctgccgcacc gtatatacgg acgcacatcc
     5101 cgcaaagaac acgccccctg aaacgccgcg cgcgtttcgg gggcgtttgc gttcgttcca
     5161 tttttttagc tgcgtgcttt cagccggaaa aatgcccgca tttcacacac agatggcagg
     5221 attcaaaaca catttgtttt tcgttcaaaa acattcctac agaaatactg tacaaaatgg
     5281 caacatctgc caggaagggt tgacaaaact ttggcagatg tataatattg atatttccga
     5341 agaatgggag taaagtaggt aagtaatatc gctttatttc ctatttatat agtatacaac
     5401 attgcgggag ggatatcatg gaaaaatttg tagaccgcat cattgaaaca gaccgcaagg
     5461 cccgcgagat catcgagcag gcccagcagg aaaagcaggc tgttctggca gatgcccaaa
     5521 aacgcgccga tgctgagatc gcccgccgcg aagccgaggc cgataaggga cgcgccgcca
     5581 tcgatgagga attcgcccag cgcgaggccc gcgagagcga gcgtatggac ggtgcctatc
     5641 tggacgccaa gcatgcgctg gacaacgcct tcgaggccgg gcatgacgca tggctggccg
     5701 agatcacaca aaatacgctg gccgcgctgt gacgcgcgct acgcataaca gaaaggggcg
     5761 aaaaggccat gcttgaaaat ctgtccgcgc ttgccatcgt acccaaggcc aaggccatcg
     5821 ccgcacgccg cctttcgcac ggcgattacc tggagctgat gcgcaagcgc agcgtcattg
     5881 aggtgacggc cgcgctgcag agccatccct attttaagga cagcctgaaa gggctttccc
     5941 aaaccaacct gcaccgcgcg cagatcgaag aggcgctcag caaggacgtt tattacaaat
     6001 acgaatcgct gatgcgctac agcttccgca agggcaagtt cggagcgtat tttttaatgc
     6061 gcagcgagat caacgagctt cttgccaagc tgcggcttct gtccatgggc ttccggcatc
     6121 attatatcat ccaggtgccc ggctttctga tgcccaaaac cagcttcagc ctgctcaggc
     6181 tggccaaggc cgaaagcgcg caggactgcc ttgaggtagt ggccggcacg ccctacgcca
     6241 aggtgctggc cggcgtgctg cccgccgcgg gccagaagct tgattatctg aaatgcgagc
     6301 atgcgtttca gtcttacttc tataccactg ttctggcgca gatcgaccgc gacctctccg
     6361 gccgcagcgc agcggacaca aagcgcctgt tccgtatgga agcggagatc tacaacctgg
     6421 acctcctgtt ccgcgccaaa gcgtttttca gcccccagct cacacccgaa gacctgaaaa
     6481 agctccttat accggtgtac ggcgtgctct cggccaaaaa catgaacgca ttggccgata
     6541 cccgggggct ggacgaattt ttgaagctgt acaacgcggg ccgcgcggga caggtatacg
     6601 gcgcccgctc ggcagacccg gcggatgcct ccgaagtatc cgaaacgcgc gctctgtacc
     6661 gcgccgcgca gaagctgctg catttttcca gcacgccgca gacggtgctt gccgcgctgt
     6721 tgtgcctggc caatctggag cgcagcaata tcatcaacgt catcgagggc gtgcgctacg
     6781 gcctttcgcc ggagcagatc agtgccttct tgaaatactg accgtatcgc ggcagccgga
     6841 cgccgcggcc atccccttcc ccgcgccggg gcgcgggagc aattgaaaag aagcgaggta
     6901 ccacagcatg ggcatggtaa aaatgaagca cattaacatc tacgggccgg agcaggaccc
     6961 ccagcagacg cttgaagtgc ttgccaagct ggcgtgtttc caccccgaca aggacgccga
     7021 gcgcatcaac gcagcggcgg gcgcggcgga aaacctgtac acgccgctgc tggccagcac
     7081 gctcgggctc atcaaggacg tgggcggaga tcctgctctg caggaatacc gcggcaggtt
     7141 cttcgaatac ggcgcggtga aggacgaggt ggaggcgctt tccgcccagg tagctgagcg
     7201 cggcaagcgc caggctgaga tcagcgcaaa gctggccacc tatgcccaga caaaaacgca
     7261 gctgtaccat ctgacaggcc tgcatacaag cgtggacgag atcttcgcct gcaaatacct
     7321 gaaggtgcgc ttcggacgca tccccaagga cagctacctg aagctgccgt attacgacga
     7381 ccactcgttc accttcagtg agtacgactt tgacggcgag tattactggg gtatgtactt
     7441 cgtgcccgaa agccacgcca aagaagtgga cgatatcttc gccagcttgt acttcgagcg
     7501 catgtgggtg ccggattttg tgcacggcac acctcaggac gcgctggcgc agatcatgac
     7561 gcaggaaagc gaactgcagg ccgagcagaa agagctggac aacatgagcg acatcgccgc
     7621 gcccgcagac atcgagcgcc tgcgcgaata cgcctcctgg ctcaactatg agatgcagat
     7681 cttcgacatg cgcaagtatg tcatcacgct ggagcacacg tattatatca gcgggtatgt
     7741 gccggaaagc gatgtagaac gcctgaagca ggggctgctg atggtgcacg gcgtgaaagc
     7801 ctgcgaggac gacgaag
//
LOCUS       DAWBSB010000098         7719 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1920_length_7719_cov_3.438935, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000098 DAWBSB010000000
VERSION     DAWBSB010000098.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 7719)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7719)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7719
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1920_length_7719_cov_3.438935"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4184..4193
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1560 a         2258 c         2403 g         1488 t
ORIGIN      
        1 gtccaagacc gtgaattttc cccactccgc cacccgcgag gaagtggccg aagtgtatat
       61 gctggcctat aaaatgggct gcaagggcgt caccatctac cgggacggca gccgggacag
      121 ccaggtgctg aacatcggca gcgtcaaaaa agaaggagga gccgccgcgc agcatccctt
      181 tggacgcatc gtgccccgcc cgcgcccggc catcactatg ggctttaccg agcgtatgaa
      241 gattggctgc ggcaatctgt acgtcacggt gaactacgat gaaaacggca tctgcgaggt
      301 gtttaccagt acaggcaagg ccggcggttg ccccagccag agcgaagcaa cggcccgcct
      361 tgccagcgtg gcgctgcgca gtggcatcag cgtggaggaa gtgtatgacc agctcaaggg
      421 catccgctgt ccgtccacaa tccgccagca gggcatgagc tgcacctcct gcccggacgc
      481 catcgctaag gttttggcca aagtggaccg tttcatccgt gaaaacaaca acgctgtcca
      541 gcatcctgag gcggagccca cgcccgggca gcgccgcgcg gcggccggcg gcccggccac
      601 ggccggaccc cgcggcagcc atgtgcctgc cgcggcgtcg gagatacata tctgccccga
      661 atgcggcggc cccgtggagc atgagggagg ctgtgtcatc tgccgcaact gcggtttctc
      721 caaatgcgga taaagctgcg tcggcataag gcggcaaaca catccccaat attttgtggg
      781 tgagcccgtg agaaaaagat ttgaagcccc atctctagaa aatatatatt ttcatgcggc
      841 agcttcaaat atatatgcgc ccaaactgca tacttcaaaa caaaagtaca tgccatgctt
      901 cgcttcgcgc cgggatccgg cgcggagcgt ttttcaacac ggacaaggaa aactttcaac
      961 atttcaaccg agttttacac cgagtatagc gggttttcca cagcttttgt tgaaaaccct
     1021 gtggaaagcg ttgaaaaggg cgtaaaatag gccccggttt tccacaatat tccgcgaatg
     1081 ttacagtttg tacataacta attgttgaat actgaaagag gttttgatac aatatcttta
     1141 tatcgccgcc ggttttttcc gcccgcggcg gattcaacaa aaagcaatat ccgcccgcgg
     1201 cgcgggcggt ggagaaagga accaaacgtt tcatgaaatg ggtctataag ctcgaacgga
     1261 aattcggccg catctgcatt ccccatctga tgctggtcat catcgcgggg caggccatgg
     1321 tttatctggc caatctgctc aacccagaca tcctgctgct cagccgtttt tcgctgaact
     1381 gggccgccgt gatgcacggc gaggtgtggc ggctgctcac ctttgtgttc attccccaga
     1441 gcaccagccc gctgggcctt gtgctgacgc tttatttcta ctatctcatc ggcaacacac
     1501 tggaaaatac ctggggcgat ttccagttca acgtctatta cttgtgcggc atgctcgggg
     1561 ccatcttggc ggctgcgctc acaggttacg gcaccagcta ctacatcaac ctgtcgctgt
     1621 tttttgcttt tgccatgctc taccctgatt ttcaggtgct gctgttcttc atcatcccca
     1681 ttaagatgaa gtatatggcc ctattctcgg gcgcgctgtg tctcatcgac ctgctgatgg
     1741 gaggctggag cacccgggcg gcggtgctgc tctcgctggc aaactttctt ctgtttttcg
     1801 gcggggattt cttccgcacg gtcaagcagg agatcaaata ccataagacg cgccgtacct
     1861 ggcagaacca gaaccgcagc aactggcgct gacacgggga atttgtgcct gcacccgcgc
     1921 ggcagtggat ttttcgtgcg atctgtgcta aaatgaaagt aagcagaagg cgcaccccgc
     1981 gccctgtaat gtttaggggg tattgttatg aaaatgatca ccgcgatcgt caacaaggaa
     2041 gacgcaaatg ctgtttgtaa cgcactgacg aagggcggct tttcggtaac aaagctgtcc
     2101 actacgggcg gtttcctgat ggcgggcaac atgacgctgc tcatcggcac cgacgatgaa
     2161 aaggtggata agtgcattga tctgatcgcc tcctgctcca agcagcgcaa agaggtggtg
     2221 cccagcaccg ccagctacgg catcggtgtc acgacggcct atccgctgga agtgactgtg
     2281 ggcggcgcga cggtgttcgt caccaacgtg gaacgctttg aaaagctgta atggctgcgc
     2341 tggattccct gctcggaaat gaaacactca aggaaagcct ctcggccgcg cttgcggccg
     2401 ggcggctttc tcatagtatc ctgctttgcg gcgaggcagg cacaggcgcg ggctttgccg
     2461 cgcgctgcct ggccgcggat tacctttacc caaacggcgg cgcctccgcc gctcaggttt
     2521 tggcgggtca aagcccggaa tacctgctgc tggcgggcga aggcgtttcc ggggatatcc
     2581 gcgtggaccg tgtccgcgag gtgcggcggg agatattcaa caccgcgctc tccgctgggg
     2641 gacgcgccgt gcacatcaaa ggcgcggaca agctgaacgg cgccagtgcc aacgccttgc
     2701 tgaaggtgct ggaggagccg cctgagggtg tgctgtttat cctgacggcg cccagcgagg
     2761 cgaacgtgat ggccaccatc cgcagccgct gctgcgccta ttccctggcc cccgtggccg
     2821 aggaggtgtg cgcggcgtat ctgaagaagc actttccaaa ggagcctgag gccgctcggt
     2881 atgcggcgct gttcggtggg aagatcggca gtgcgaagcg ctgcctggag gacgcggccg
     2941 ggcggacgct tttgctggac gcgctgaagc tggcgggcct ggccgaaaaa caggacgttt
     3001 acggcgcatt ggcgctgctt tccaaatatg aaaaagaccg cgcgggcaca caggcgcttc
     3061 ttggcctgct gctgaacgtg tgcagcgctt cactgcgggg cagcgaggcg gcccaaagcc
     3121 ctgtaagccc ggcccgcgcc gcgcgctgcg caggggaagc cggaaaaacg gcccggctgc
     3181 tggcaggcaa tgtcagcggt aagctggcgc tcacctgcct tgccgcgcgc ctgacggcat
     3241 gagcctgcgc tttgggcggg agggctttgc cggcgggcgg agactttgcg cggatatttc
     3301 ttccccgcgc ccccactctc ttgataaaac gccgcttcaa atgccgcggg cggcggaact
     3361 gacacccatt gcagcggcgg cgaaaggaaa gaagctatga caaaagtgat cggcgtccgc
     3421 ttcaaagcga acggcaaatc ctactatttc agccccggcg agctggaact ccaacagggg
     3481 gaccacgtta tcgtggaaac ggcccgggga acagagtgcg gcgaggtagc aaaaggccct
     3541 catgacgtgc cggattccag catcgtcaag ccgctcaaga cggtcacgcg catggcggac
     3601 gccgtggatg tgcgccgcat gcagcagaac cgcgcggacg aaaaacgcgc cttttcggtg
     3661 tgcgaggagc gcattgccaa gcacaagctg gacatgaagc tggtggatgt ggagtatacg
     3721 ctggaccgca acaagatcct gttctacttt acggccgacg gacgcatcga cttccgtgac
     3781 cttgtcaagg accttgccgg tgtgtttcgc acgcgcatcg agctgcggca gatcggcgta
     3841 cgggacgaga gcaaaatgct gggcggcttg ggtatttgcg gccagccgtt ctgctgcagc
     3901 cgttttctgc gggatttcca gccggtgtcc atcaaaatgg ccaaggaaca gggactctcg
     3961 ctcaatccgg ccaagatcag cggcagctgc ggccgcctga tgtgctgtct tgcctacgag
     4021 cagccggcat atgagtatct gaacaggatc actccgggcg tggggagcat tgtcaaaacg
     4081 cctgagggag tgggcgccgt ggtggagacc aatgtcatct ccggtaccct gcgcgtgcgg
     4141 atggaccccc cagccaccgg cttcaaaacg ttccacaagg acgnnnnnnn nnnacgaatg
     4201 ccagtatctg cgcggcggca agcgcgcacc catcgcgccg gacccggagc cggaagagat
     4261 caccgaatga acgcttttgc cccgcgtagg tttgcgtctg ccgggccgtg cggggaaaag
     4321 ctgtttcccg gcgtgtaaaa agtgcgtgaa agtggccctg cgccccttga aaaatccgca
     4381 ttttatgtta aaatgagaac acatggaggc acagcctctg gcggctgcat gcgctgaccc
     4441 gcgccgctcg cgcacgctgc cgtaaaaaat tcgatcgcgc ggagaagagg atggatactt
     4501 atggcttaca aaatttctga cgactgcatc gcttgcggca cctgcgctgg cgtgtgcccg
     4561 gtttctgcga tttccgaggg cgacggcaag tatgtcatcg acgcggacgc ctgcctggat
     4621 tgcggcacct gcgccggcga atgcccggtt tccgctattt ccgagggttg atttcccggg
     4681 aaaagcagag caaagcaaca aagaggcggg caccgtgcgt gcccgccttg ctttttcctt
     4741 attcaggcga tttcggcctc tgcatccgcg aggggcctga atttttgatt tgagcggcct
     4801 cctctacttg ggcgtgccgc tgaaaccgat tcgaaaggag cctccgcctt tgcagcagga
     4861 acaccttgcc aacggcacac ccgttttcat cacgcaaagc caccgttttg gccaggatgc
     4921 actgctgctg gctcactttt gccgcgtgcg ccgcgccgag gctgtgtgcg atctgggcac
     4981 cggctgcggg gtcattcccc tgcgctggca cgacagaggg caccgcggcc catgccttgc
     5041 gctggatatc gcgcccggcg ccgtagcgca gctgcacgcg gcggtgcagg ctgcggaggc
     5101 ctggcatatc acgccggtca cagcagatct gcgggacctt gcaggcctgc acccatatgc
     5161 ccagagccgc gatgtcgtgg cctgcaaccc gccctatttt acaggtgggc gcgtcagtcc
     5221 ggacgcggcg cgggcgggtg cgcgccatga gctttcctgc acgatgcccg atgtatgcgc
     5281 ggctgcgggt ctgctgctga aagacggcgg caggctgtgt gtgtgccagc gtccggaacg
     5341 cctggcggac gtgctgtgcg caatgcgcgg cgcgcgcatt gaacccaagc gtctgcaatt
     5401 tgttatggcc cggcctgaaa aagcgccctg gctggtgctg gtggaaggcc agaaaaaccg
     5461 cgcgccgggc ctgcgcgtgc cggccccgct cgttaccgcg cgggcagacg gccgccccag
     5521 cgcggccatg ctggaaattt acggaacaga accgaaaaag gaggccccat gagcggcact
     5581 ttgtatatcg tggcaacgcc catcggcaat ttgggcgacc ttaccccacg cgcggcggaa
     5641 acctttgcaa aagcggattt tattgccgcc gaagacaccc gtgtgacgct gaagctgctg
     5701 aaccacctgg gcctgaaaaa gccgatggtg agctattatg agcacaatct gaaagagcgg
     5761 ggcggctata ttctggaccg catcgaggcc ggggaatcct gcgcgctgtg cagcgacgcg
     5821 ggtatgcccg ccgtttccga ccccggggag gtcatcgtgc gggacgcaca cgcccggggc
     5881 attcccgtag tgcccgtgcc cgcggccagc gccgctgtca cggcgctgtg cgtttccggg
     5941 cagccgacgg gtcggtttgt gttcgaaggt tttctgccta ccaaccgccg tcagaaaaag
     6001 gagcggctgg ccgcactgca aaacgaggag cgcaccgtga ttttttacga ggcaccgcat
     6061 aaactgccca cgactttaca agacttggag caggcgttcg gaccggagcg gaacctgacc
     6121 atctgccggg agctgacaaa gctgcacgag gagatccgtg tcaccaccgt gggggatgcc
     6181 agcgcttact ataaagaaaa cccgcccagg ggcgagtttg tgctggtgat ggccggcgca
     6241 cagccatgcc ctgcgcagga actgacgctg gacgacgccg tcggcctggc gcgaaaacag
     6301 atggccgcgg gccagagcgc cacgtcggcg gcaaaatatg ccgctgcgca cagtgcattt
     6361 tccaagagcg aaatttaccg ccgctgcctg gagtaaggcg cggcctgaaa ggcggaaagc
     6421 atatgaagct gctggtcgtt tccgacgtcc acggagattt ggacgcgctg gaacgcgcgg
     6481 tggatgccga agcggatgca gacgccgtta tttttttggg agacggtctg cgggaagcgg
     6541 aggacctgca ggatatgcgc ccggagctga agatttacag cgtgcggggc aactgcgatt
     6601 atgcctcctt tgccccgccg gacgggctgg ccgctttcgg cggtgtgtta ttttattata
     6661 cacatggtaa tttatataat gtaaaaaacg aactggatac acttgctgat gcggcacgtg
     6721 cacgcggtgc ggacgcagcg ctttatggac atacccactg tgctggctgc gaggagcgga
     6781 gcggtgtggt gcttttcaac cccggcgcgc tttcccgggt gcaggggcgc ggcagctatg
     6841 gtgtggtcat ggtggaaaac ggcgcgccgc gttttgagca cagacgggtt tgatacgcgc
     6901 ccgtcccgag ataaaggaga gcggcaactg taaaaaccgc cgcccgcgca gccctcataa
     6961 gagggaatcc gcgggcggcg gttttttgat tgttccgtaa tgcttacccg gttggacgcg
     7021 ggacggcatg caaggcctga aaaaacgacc tcgatgaccg tggcgggatg tacgatcaga
     7081 gcagatagcg aaaaatttca tcccgctgct cgtattcaca cagcacagaa aggccgaagc
     7141 agaatacacg ctgtgcttcc gacaacgggc ttggcagcgg cgcgtcctcc aatgcacagc
     7201 gcgccagcgc atcctgctgg gccggggtga gcagagcttc cgcttcctca aacgtcatag
     7261 ccatatatgc tcatcctttc cggcagcgtt cagtcggcga acagcggcgt ggaaagatag
     7321 cggtcgccgg tgtcgggcag cagtgccacg atggtcttgc ccgcgttttc cggccgtttg
     7381 gccagctgaa tggcggtcca gacagccgcg cccgaggaga tgcccaccag cacgccctcc
     7441 agacggccca ccagacggcc tgcggaaaag gcgtcctcat tctccacggg tatgacctcg
     7501 tcataaatat gagtgtccag aacttcgggc acaaaacctg cgccgatgcc ctggatcttg
     7561 tgcggcccgg ctgtgccctt gctcagcacc ggagaggccg caggctccac ggctattacg
     7621 cggatggcag ggttctgcgc cttgagataa gcgcccacgc cggtgacggt gccgcccgtg
     7681 cccacgcctg cgataaagat atcgacattg ccgtcggta
//
LOCUS       DAWBSB010000099         7689 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1927_length_7689_cov_3.228583, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000099 DAWBSB010000000
VERSION     DAWBSB010000099.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 7689)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7689)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7689
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1927_length_7689_cov_3.228583"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5972..5981
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1542 a         2116 c         2426 g         1595 t
ORIGIN      
        1 taagggcggc gtgctgctcc gccagagggc atatcctgtg aaggtcgtgg acacaacggc
       61 cgcgggagat acattcacag ggtattttct ggcaggtatg atccgcgggt ttgacccggg
      121 aaaatgcctg cgctgcgcaa gtatggcggc tgcgcttgca gtaagccggg aaggcgcttc
      181 ggcaagcatt cccacatgga gggaagtatt ggatttcagc gcggcgcagg gggaaccgct
      241 ggagagctta cggggaacag gagccgcggc gctttgacag gcattggcac ggcgcgtcca
      301 tggaaaaaag ggctgccggg aagcgccgcc ggatgacgga agaaagaggc gaatggcatg
      361 aacacagtca cagtgatccc ggcggtgcgc gtatccgaac aggtccagcc ggaatttcgc
      421 gtattttccc atgaggcgga tgccctgcag atcgacgtgc tccggggcgg atgcatattg
      481 ttgggctgca cgcggaagct gacgccggga aaaacatcgg tgtttcgtta cagcctgaag
      541 ggggctgtgg gcgatgtgga gctgcgcttc cggttttaca ggggagagaa ggaggtggag
      601 acaagctcgg cgccgtatca ggtcgttccc ggcagtgtgc ccgccacagg cctgatcgac
      661 ggctgctgga tatccatctg ccactggagc gaacaggaag cggtgttctt taattcggaa
      721 ctcaaaaaaa tgcgggacga ggatttcgaa cggcatatcg aggagatgtc ccttcttgga
      781 atacggggcg ttgtgatcca gaatgtattt gattcctccc attatgtggg ccgccatgag
      841 atgacctgcg ggagctatga cggccgggca ttttatcctt ccgggctcta tcccggaaga
      901 atgccgcttg ccgcgcggga ccccattgag gcggtgctgc gggccgctga ccggtgtggg
      961 atgcatgtgc tgctgggtgt gggcctgttc gcctggtttg atttttcgga acagtcgctg
     1021 gagtggcaca ggcgcgttgc acgggagctt tttcagcggt atggaggcca tgcgtccttc
     1081 tatggctggt atgtttcaga agagatcatg ggagacctgt attattcgta catgccgcac
     1141 tccgcagagc gctggaagga gcttgccgtg ttctttcacg agttccgtac ttttgtacag
     1201 gaacttgctc ccacaaaacc ggtggctttt gcgcccaata atgtacggtt ccatctttat
     1261 gcggaggaat ggaaaaccat cctgcccttt attgatattt tgatcccgtt tgcttttgcg
     1321 cgcgatccgg aacatctgaa tgtcgtgcag atgaagcaga tctgcgacga atgcggcacg
     1381 cacatgtggg tggatatgga gatgttccgg gagccctttg aggatgggct tgtacccaag
     1441 acgatggaag cgctgcttcg tgagatccgt caatatgacg ctctggagca gtgctacggc
     1501 taccagtata cggggctgat gaaccatccg gcctcccgct tttctctggg aggggagccc
     1561 gcaaaggaat tgtatgcccg gtatttacaa tattatcggg cgcgccgtgc tgcatgggga
     1621 tatggggaaa aacagcaatg atttccgcgg ctcgaaaaag aggcccggcg gacaaaatcg
     1681 gagggaaaag tatgaaggag atactcggcc gcatcgttgc ggcagaattg gaagaacgct
     1741 ggaagcaggg atacgatgtg gaagcgctgc ggcctcttct gggtaaggcg tccggcagct
     1801 gtgacgcgct gctggcgctt tcacagcgta tcaaagaggc gcccatacgg caggattggc
     1861 cgtacgagga gccggacgac ctggaaagcg tgatggcggc gtgtgaccca acgcgcaggc
     1921 gggaagcgtc ccgtctgctt tccgatgggg agatcgaggc gcgcgtccgc tccgcgttcc
     1981 tgacttcggt ctgcgcatgt atcctgggca agcccctgga ggaggctccc tacggcggac
     2041 tcgaggacat ccgcgccgcc gcgcaggcaa gcggagaatg gcctctgcgg gattatgtga
     2101 gcgatgcgat gcttacggcg tgggggaggc ggaacccatc ctgggtggaa acgacccgag
     2161 gaagggtgcg ctatgccgcg tccgatgacg acatcaccta taccatcatg ggcatgctgc
     2221 tgcttgaaaa gcggggaagg gatttttcgc acgaggacat gcgtcagctt tggctggaga
     2281 accttccgat ctatttgtgc tggggccccg agaggacggt gctgctgcgc gccggccttg
     2341 cggttttggc gcccgatctg ccttatgata tggacgattg ggcggtgcgg ctgaatccgg
     2401 gccaggagct ttgcggtgca atgattcgtg cggatgccta tggctatgcg tgtcccggcg
     2461 acccggagct tgccgcacgg cttgcatccc gtgacgcatc ctttacacac cggaaaaccg
     2521 gcgtatacag tgcaatgttt gttgcggcgg caattgccgc cgcgtttact gcggaaaatt
     2581 ggaaggagat cgtcgtcacc gcgctgcagt atgttcccca gcgcagcaga ttttacgaac
     2641 aggcttccac ctgcctgcgc atggtggatg acgccgcttc tttcgaagat ggttataacg
     2701 cggtccatac cgaatacggc gcgtttggag cggggcaggt cgtacaggag atcggcacag
     2761 tcatcaatac cctgaagttt gcccgggatg ctgaggaggg aatcggcatg caggtggcgc
     2821 aggggaacga ttccgacagc tttgcctgca gctgcggttc cattttgggc gcttatttcg
     2881 ccgatggcct gcctgaacac tggtatgcgc agttcaataa tacgatacac acgacaatgg
     2941 ggaattttca cgagaccgat ctgcaggctc tgacaaagcg catgcagctg ctgtacaagc
     3001 gcgttcggca gccggaagca ccgcttgcct gagtgcgcgc ccggttcatc tccgtccgat
     3061 tggcaggctg ccgctcccgt gccctttatg ccgcggcgtt ggcataaagg gcttttttat
     3121 ccgctccggc ggaattttct gcaaggcgcg cagtgggggc gggacttgcg cgaaactgca
     3181 tgattttatc ctttttttgc atagatttgg gctgtttatt attgtatatt tattgtactg
     3241 aaggaagaac cgggcattcc cgactaaggg aaaacagcct ttttctgtcg gctgcccggc
     3301 tatttactgc gaaaggtgtt ttgatatgga ccccattgta aaataccctg atttcacaca
     3361 gctggacgcg ctgagcacac ccctgcggga atatgccaga ttgaaatgcg agcagggagc
     3421 ggaaacaggc gccgccgcac tgatcggtca attgacggag accatgcgct gtacggtccg
     3481 gcttctgcag gagctgccgg acgatgccgc cctgtctgcc tgtgaaccaa acgaactgga
     3541 cgccatccgc gcgctgcgtg caccggcgca gcgccggctg tgagaggcgc tgccgcccga
     3601 ggatatctgg atggacaaat tggagggcgc gctgctgggg cgcatcattg gctgcctgct
     3661 gggcgtgccc gtagaagggc tctcggtgga aagcatgcgt gcatggagcg agtacatcgg
     3721 caagcccttc ccgccggttt cctattggga agaagtgcaa aatccggagc tttggaactg
     3781 ctacggccgt cagcgcggcg aataccggaa atgcgcgctc cggtccgctc cggtagatga
     3841 cgacctggcc tatacacagc ttgcgctgct gattttggaa gaatacgggc ctgattttac
     3901 gaccgaggac gttggggagg catggaaaaa atatcttccc gttgcctgta cggcggagca
     3961 ggccgctttg gacaatctga aaaaaggcgt gccggcgcgg gaggctgcgg acagggataa
     4021 tccgttctgc cagtggatcg gcgccgccat ccgttctgac ggatttggct gggcagcggc
     4081 cggcctgccg gagctggcgg cagaaatggc ctggcgcgac gcccgcctga gccacaggcg
     4141 caacggtatt tacggagaga tgttcctggc ggcggcacag agtgcggctt ttgctgtgag
     4201 cgaccccctg gacgccgtgc gggccggcat ggcggagatc ccacgggaat gcctgcttta
     4261 ccgggacctc gcctgggcgc tggagcattg cggcgcggtg cgcggccacg gcgatgcgcg
     4321 ccggctggtg gatgaacggt tcgcgggaat gcatgccgtt cacaccaaca acaacctgtg
     4381 tctgatcgtg tttggcctca agctggcgga gggagacctg atgcggggac tgtcccagac
     4441 tgttgcgatg ggccttgaca gcgactgtac cgccgcctcc gcaggcagca ttctgggcgc
     4501 ggttttgggc aagagagcga tcgccccgga gctgtacgag tgctttcacg accgtatgga
     4561 tacttacctg aagaacacgg aggcgttttc gatacgggat atggccaagc ggtattccgc
     4621 gcttgcacac aaagtgcatt cccggctgtg accgccgcct taggaacatg ccgccggaaa
     4681 aacggggagg ctgctgctgt tgcaggcgtt cgccccgttt ttccggtttt ctatggccgt
     4741 tcaaacggct gaacgccggg attcgcccgg aagatgagta tttctgctga ttttgcaaat
     4801 tgctattgtg cgcacagtac agtttgggat ataatataaa acggccttga ggcaaggccg
     4861 ttttttgtgt tgcaatttta catcaataaa agaggggtta gtacaaatga gaactgttgg
     4921 caccacttcg cgcggcgtac gctgcccggt cgtccgtgag ggcgacgatc tggtcaaaat
     4981 cgtcaccgac gccatcctcg cctgcggtga agagcagggc ctcaccttcc atgaccgcga
     5041 tatcgtggct gttacagagg ctgttgtggc acgcagccag gggaactacg ccggtgtgga
     5101 ggacatcgcc gccgactgcc gcgcaaaatt cggcgccgat acggtggcac tcacatttcc
     5161 catcctcagc cgcaaccgca ttgccatctg cctgaaaggc attgcaaagg cttttaaaaa
     5221 agtgtatatc cttatgagct atccctccga cgaggtggga aaccatctgt tcgacgagga
     5281 cctgctggat gaaaagggcg taaacccctg gagcgacgtg ctggacgagg cgaaataccg
     5341 cgagctgttc ggctatgaga agcatccctt caccggcgtg gattatgtgg agtattacgg
     5401 agacctgttc cgtgcggagg gctgcgagcc ggtattcctg ttcggaaatg acgtgcgcgc
     5461 cgtgctgccg tatacaaaga atgtgctgtg ctgcgacatc cacacccgcg agcgctccag
     5521 gcgccgcctg ctggctgcgg gcgcggaaaa ggtgctgctg ctgagcgacc ttatgacggc
     5581 cccggtgaac ggcagcgggt acaacgagca gtttggcctg ctgggctcaa acaaggccac
     5641 ggaggaaagc gtgaagctgt tcccgcgcga ttgccgcagc tttgtggacg cgcttgcaca
     5701 gagcctgcgc agcgccacgg gcagacagat cgaggtcatg gtgtacggcg acggagcctt
     5761 taaggacccc gtgggcaaga tctgggagct ggccgaccct gtcgtgagcc ccgcatacac
     5821 cagcggcctg gagggccagc ccaatgagct gaagctgaag tatctggcgg acaacgattt
     5881 tgcctctctt tccggcgatg cgctgaccag cgccatcaag gacagcatct cccacaaaaa
     5941 tgcggatttg aagggccaga tgtccagcca gnnnnnnnnn ngcaaaagcc cccccatccg
     6001 cggatggggg ggcttttcta ttgcgaggct gtttgcccgc ccggcgcaca tccgggcggc
     6061 atgcccttac agcaggccgc gaatcagctc tgcggcatcc gcaatgtatt gggccagcgg
     6121 cttttccgga aggcccgcaa tgtgcgcccc gcatttggca acggggatga gcaccttgtg
     6181 agccgggctt tccgccacgg cgcgggccat ggccgggctg cattcgccca gcatgctgtt
     6241 tgccatcaca aggccgatgg ggcccgccag aatacgcgcc cgcgcggcgt tcacgatcac
     6301 ggcgttttct cccgtggctc cggcggatgc acccgcccgg agcatggcgc tggtggccag
     6361 cgcgttggtg cccaccgcca cgatctcctg cgtcacgccg cccgcgcgca gcgcctcgat
     6421 gagcgcgcgg ccaaagccgc ctccctgccc gtcgatgata acaatatcca tattccgtcc
     6481 tccttacatc agcggcgaga agaagcgcag gaacacctgc gccagccatt tgtacagcgg
     6541 cgtgcgcagc acatccgcca tcgttacctc atggctttca gccagcgtgg cgcgcacgtc
     6601 ttccagcacg tcccgcacca tatggccgcc gtaaaacgcg cagcagtttt caaagtgcag
     6661 gtacaggctg cggtaatcca tgttggcgct gcccacaatg gcctcgcggt cgtcgctgac
     6721 atacattttt gcgtgcacga agccgggggt gtactcgtaa atgcgcacac ccgcttccag
     6781 caggcgcgga taatagctct gcgtcacata atacacatac catttatccg gcacaccggg
     6841 cgtgaggatg cgcacgtcca cgccgctttt ggctgcaagg cacagcgcgg tgaccatctc
     6901 gttgtccacg atcaggtagg gcgtaacgat gtacacataa tcccgcgcgt gctgaagcac
     6961 gttcagatac gcgctttcgg ccacggcttc gccgtccagc ggagaatcac aatagggctg
     7021 cacatagccg tccgcttcgt attcgcccag gggcatataa tcggtgtacg gggtggtggt
     7081 gcccgccaca aagtcccaca ttttcaaaaa tgttacggta agcggataaa cgcccggccc
     7141 tttcagcatc aggcccgtat ccttccacac gccgcagcgt tgcttgcggt tgatgtactc
     7201 gtccgcaaag ttcagcccgc cggtgaaagc tgtttcaccg tccacgacgg tgatcttgcg
     7261 gtggtctcgg tggttgagca ttttatagtc agagatatgc agcgaaaaat gcagcgcatt
     7321 gaatacatgg cacttgatgc ccgcgcggcg tatctcttcg tcataatcac cgggcagtgt
     7381 gaacatggaa ccgaagccgt cgtagatcag gcgcacgtcc acgcccgcgg cggctttttc
     7441 cttaagcacg gccagcgttt cgttccacat ttcgccttct tcaacgataa aatattccat
     7501 gaagatatat ttcttcgcgc cgcggatgac ctccagaaaa cgggcgaaaa aatcctgccc
     7561 catgggataa tactcgctct gcgtgttgca gtagagaggg gccgaggcgt tgcgggacag
     7621 atattccacg ctgcggcgaa aagcgggctc ccgggcgtac agggcctctg ttacagcatc
     7681 gtcctgcac
//
LOCUS       DAWBSB010000100         7659 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1936_length_7659_cov_3.118490, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000100 DAWBSB010000000
VERSION     DAWBSB010000100.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 7659)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7659)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7659
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1936_length_7659_cov_3.118490"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    218..227
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1802 a         2131 c         2391 g         1325 t
ORIGIN      
        1 ccagcactat cagccggtac ggcaagcgcc tgagcgaaac atatcgggat ctgacgattg
       61 ctcaggaaaa ttttgaggca ctgcgcaagg aagtggaaaa atatccggaa caggacagcg
      121 cagagatcct cacccgcatt gccagccaca aaatgatggc ggccctcgtg tccaagtccg
      181 acaaagactg gaatgagatg cgggccgata aacttttnnn nnnnnnnaag aggggctgta
      241 gcatggaatg gtatgtgctg caggccctca caggtcggga acaggacgcc agggcggcgc
      301 ttgaacgtgc cgggtttgac gcccgggtgc cgacggaact gcggccgatc cacaggcggg
      361 gccagtggat cgaacaggaa tacacgctcc tgccgggcta tgtattcgtt gggatggatt
      421 ttgagccccg gctgtatcgt gccatcaagg aagtgccggg agccgtccgg ctgctggggc
      481 tgtttgacga acatcccacg ccgctgccgc aggatgaggc ggcactgtgg ggcctatgtg
      541 gtcccccttc tgctctcatg ccaagcgtcg tagcgttcga cgcctacggt acgccacggg
      601 tcatagatgg tccactgacg ggacgtgagg ctcagatcgt gaagatcgac cgtcatgcgc
      661 gccgggcgcg cgtactggtg gataccggag acgaacggca tctggtgcgg ttcggtatcc
      721 ggcctgcgga cggtgatgcg cagtgaatcg taaaaaggac ttgcggcgtg acgccgcaca
      781 caggctcgcg ggctacggtc cggaatatcc tccagagctg tgccggcggt gcgtctgggc
      841 gtccggccag ggcgaccgca agctctgccc gttcccccgc tgcgtagtgc gcagcggaga
      901 tatgaggctg aagccataag gctgttgttg aaaagttgaa acgggcgtta aaactcgttg
      961 aaaactttgg cagcagggta tttaaatgcc gggttacaga aaatttaaaa ccggcattaa
     1021 acagaacaag aaaaccatga aagcagcggg gtgattcgtc ccccgcccgt ggccatggcg
     1081 ggcatagagg ggtaaaaacc gggcggaaac ggccggatgg cgaagcatgc ccggtgaaaa
     1141 tccgcccctc ttgcccgccg cgctgcggaa taccgtttaa tttcccctgt ataccattta
     1201 aaaccgctca aaagctccgg gccgtgcaga cggatacctg ggggcttttt tgcgttacag
     1261 gcgaaatttg gagccgtgca ggaaccagga aggagatgcg tggaatgaaa cgtactcaac
     1321 agagcagcgt agacgccctt ttaaaagctg tcgaggcagc aaaaacggaa aaccagtaca
     1381 acatttttaa ggatttaaag atgctgctgg aagcgtaccc caaaacgagc aagcgagatt
     1441 acctggcgct gatccgcgca ctgatggaca aatacagcgc aggcgtcggc gcagaaatca
     1501 atgacgctct gataaagaaa tgcattgctg gcgacgtcga agcgattcgg ctatacaagg
     1561 acctgcaaaa agaggatcag acggccggcg aggaggtaca gatcattgac gacatcaaaa
     1621 ttaagtgaat gcatgtcccc cgcgttctac gagctgcaca cgcagatcaa gacacagcgg
     1681 ccacataccg tcgtatgcaa gggcggccgc ggcagtacaa aatccagttt catgagcgtg
     1741 gagttcatcc tgttccttct gcagcatccg caatgtcatg gcgtggtgct gcgcaaaatt
     1801 gccaatacac tgcgcaacag tgtgtacgcg cagatccagt gggccatcgg cgcgctgggg
     1861 ctgcagaaca agttccggtg tacggtgtcc cccatggaga tcacttacct gcccacgggc
     1921 cagaagatcc ttttcttcgg cctagacaag ccggaaaagc tcaaatccat caagctgccg
     1981 tttggataca tcggcatcga ctggtttgag gaggccgacc agttttccgg tgaggcggag
     2041 atccgtaacg tcaagcagtc cacgctgcgc ggcggcgagt tcgccatgac gtttctgtcg
     2101 ttcaaccctc cggccgcggc gcgcaactgg gcaaaccggt atgcactgga gccggagccg
     2161 ggcaagatcg tacaccacag tacatatctt accacaccgc gggactggtt gggccagcga
     2221 ttttttgatg acgcggaatt tttgaagaaa acgaagccca cctcttaccg tcatgagtat
     2281 ctcggcgagg ccgtgggcaa cggtacgcag gttttcgaca acatccggtt ggaatccatc
     2341 acaaaggcgc agatcaactc tttcaacgag atcacctcgg gcgtggactg gggctggtac
     2401 ccggacccgt gggcattcaa ccgcatgtcc tacgacgccg cccgccgcac gctgtacatc
     2461 ttcgatgagc tcacccggcg aaagctcaac aattttgaga ccgcgcgcct ggtccagtcc
     2521 cgcattccgg agggtgagga tgtcatcgcc gattctgcgg agaaaaagag ctgtgcggac
     2581 taccgcgact gcggcatccg gtgttttgaa gccaataaag gccccggcag tgtgaatcag
     2641 tccatgaagt ggctgcaggg actttccgct atcgtcatcg accctgtgcg ctgcccggat
     2701 acggccaaag agttcagcga gtacgaatac gagacaacaa aggacggaga ggtcatgtcc
     2761 ggctacccgg atgcggacaa ccaccacatc gacgcggcgc gctatggcac aaaccgcatc
     2821 tggacacgga ggggtgcgtg atgggacggc tgcgtgactg gctcgtgggc aggtatctgc
     2881 ccggctgggc cgtgcagacg atcctggaag aaaacagacg gctgcgcagc caggccgagg
     2941 agctgcagga ggaaaaccgg acgctgcggg catatacgga cggtctggag catgccctcc
     3001 ggcacggcat gcatgttgag atcaatgtag agggaggcgg tgcacatgct gatcggagcg
     3061 ctgaaggcgc tgacggataa cagcgtcata aattcggcgc agggcttcgg ttcgccggac
     3121 gtcaccacgt ccgccatgcg cgcggccatc aaagagtggt tcggaacata ttttcgtcag
     3181 gaagccggaa ataaaaaaga cccgtgcacg cgccttgcgt ataccatcgt gcataagctg
     3241 gacaagggcg ttttcgctga gtacaaatca gacatcctgg acaaagagaa aacggccaag
     3301 ggcgcatgga tggacgccaa cctgtctttg ctggatctgg ccaaaagcga tatcatgcag
     3361 tggatgctgg tgggcggcga agtgtatgtc aagccggtgc ccaggactgg gccggacgga
     3421 aaaaccgtgt tccgccctca gcccatccgg cggaacaggg cggccatcct ggcacgggac
     3481 ggcgatggtc atatcacgag catcggcacg gcggaggaaa cgaccagcgg cgggaaatat
     3541 tacacgctgc tggaaaagcg caccgtggac gcggcaggct atctcaccat tgagaacaag
     3601 ctgtttgagt catccgaccg ccattcgctc ggcgtccgtg tttcgcttgt gcacctggca
     3661 caatacgaag cgctgccgga ccggtatacc tatccccgcc ctgtgggcgg gctggggctt
     3721 gcggtgctgc gcgtgccgct ggtgaactgt gtggacggca gcgcggacgc cgtgagtatt
     3781 tacgagccgg ccatgcaggt catcaaaaac atagaccaca tggaatacct gcacagcaag
     3841 gagttcgaac tggggcgcca ccggatcgtg gcgccgggcg agatgctgcg cagcggtccc
     3901 gacggtgaac ggcggcttga gctgcgcagg aaagccgccg cgatagcgga gccgatttgg
     3961 gagacactgc tgcgtgatat cgtgcggcgc gtgcgcggcg ccggcggcat cacgtccaca
     4021 gcggaatacc agatataccg ggccgagcag ctcggcctcg ccgaaaaggc catcaaggcg
     4081 gcaattgcgg agcagctgga aatctcggac gccgccattg acatgctgtt cgaggagctg
     4141 cgggacgaaa cggtcttgtt cgaggaaaac gcggagctgc gccagctggt ggaagcctac
     4201 ggcacggtgt cgaaaaaagc agctgccgcg gactatgagg ggctgtgggc gcctggaccg
     4261 gacggcaagc tgtacacggt gaaagaggcc tatggcaaga tcatggattt tgcatggatg
     4321 cagaccgcca cgggcacata tgactttcag accgcggtcc gggaggcaac aaaaaagctg
     4381 ctggaacggg gcctgcgcgt cattcccggc aaagacgggc gaagctaccg tcttgaatat
     4441 gcggtgcgca gctacatcac gaaccgcatg ggcgagatgt tcaacgcggt caaccagatg
     4501 aactacgacg ccatcggcgc ggacggctgg gagatcagcg cgcatcctgc cccggcgcct
     4561 gaccatgcac tctatcaggg ccgtcaatat tcgaaagaag aatatgaccg gatcaacaac
     4621 agccttgccc gaaaattcgg gtggtggaat tgtcggcatc ttgtgtatcc catccggctg
     4681 ggcgtcagcc cgcgcgcata cagcgatgag cagctgcaga agtatctgga cgacaacgaa
     4741 aaaggcgtct ggtacaatgg gcagcactac acgctgagcg aggccaggga ccgcaagcgc
     4801 cagcttgaga gcctgatcag ccagacaaaa tacgatatcc tcgcagctga gggcgacgcc
     4861 gagctgctga aggaacgcca gatccggctg cagaaccaac gccgggaata cgatcggttt
     4921 tgccgtgaga ccggacagga gccggagaac tggcggacca tggtggcggc cttcgggcgc
     4981 agcgaggcca gcaaggccgc gtgggcggcc agaaagctgg gactgcgcgg ccgcgcatct
     5041 ggtaaaacag gcggcgcaga tacagacggc gccccaatgc gcgccggaca tgtggacttt
     5101 tccgatcgtg aacaggtctc atccaccctt gcaaaatggc agcgggacct ggagccgttg
     5161 gaccatgagg aagacgtctc catcacgacg gacggaacgg tctggcatac ggtgggaggc
     5221 aaacgccggg tacacccgga aaacatccag ctgcagcagg gcgtttccct gcatgggtcg
     5281 tattcgtacc acaaccaccc gccgaaagag acatactaca gcttcagcgc ggatgatgtt
     5341 gatttcttct tccggttcgg tgtacaatac cagcaggcgt cggatgacgt ctacgagtac
     5401 agcatcgagc gtacaccgga cacgatcgtt cccgagaacg ctgctgcgga gtttgatagc
     5461 ctattgcgtc aaatcgggtt cgagaatgaa ggcccagact tcgatgtcga tgttgaaggc
     5521 tatgatcttg taatgcagca gctggcagaa aagtataggt ttcaatatgt ccggcgcagg
     5581 aaggagtgac cgctgtggaa aatgtcccga aggaccttga agcattccgc cgtgaaatgc
     5641 gggagatgcg agagatctac gagcagaggt ttgctgagct ggatgaaaaa tacaaggacg
     5701 acccgtcgcc acgcggcttg gatgataggc cgggtgcaga tgagtacaat gcgctcaccc
     5761 gccagatgtt tgcggatatc caccgtgtgc gccgcaaaca cggtttcaaa cagtagcttc
     5821 tattaaatat ggtagatagg gacccattcg tgggtccctt ttacaatgcc ctttaaagca
     5881 cctgtaaccg gtgttttaag ggcattttta aattgctttt accgctggcc cggcggacaa
     5941 caaaacgggc gccgcagtac cgggactggc cggataaaaa ggacagcggc tgaccgacta
     6001 cccacccatc atccgaaagg agcaaacaaa aatgacgttt gactggctga aaactctcct
     6061 cggggacaca tataccgagg acaccgacaa ggccgtcgcc acagagatcg gcaaacgctt
     6121 cacggcaaag cccgattttg aggccaagtc cacggagctg aagaacgcga aggcccagct
     6181 ggcggaggcc aacaagacca tcgaaggcct gcaggctgcg gataaggaca tcgaggccgt
     6241 gcgcaaggag gctgcggact acaaggccaa ggctgagcag gcggaaaaag acgctgccga
     6301 gcagctggag tcgtataagt tcaacgcctg gttcgacggc ctggtggcac agaaccacgg
     6361 ccgtgacggc gcggtgatcc gcacgctggc gggcacggag cgtatggatg ctttgcgcaa
     6421 gagccagaac cgggacgaag acggcaaggc cctgtttgac gatctgctga agaacagcgc
     6481 ttacgctttc gaggaccaga cgccgcctcc gcctccctat gccggcggca caggctccgc
     6541 ttccgttgcg gcggacgatg ccgccatgcg cgcggccatg ggcctgcccg cagagaagaa
     6601 ataacgttga gaaagagagg aaaaaacaat gccgaacctt attgaactcg caaaatccta
     6661 cgtccccatg ctggacgagg tgtacaagct ggcgtcctgt acgtccgacc tggacggcgc
     6721 gccggagctc gcgcgccagg gcgctaacgc caacgagctc atcatcccca tgctggagat
     6781 ggacggcctg gccaattacg accgcaacgg cggatacgtg gacggcgacg tcaagatgga
     6841 gaaccggacc gtgcagtgca atttcgaccg cggccgtatg ttcactgtgg atacgatgga
     6901 caatgccgag accgcgggcc tcgcttttgg ccgcctggcc gcggagttca tccgcaccaa
     6961 ggtcgtgccg gagctggatg ccttccgctt cgcctgctat gctggcaagc ctggtaccag
     7021 ttccgcagag ggcacgctgg caagcggcac ggacgtgatc gcggccatca gcgccgctgt
     7081 gaccgagatg gacgaaaatg aggtgccgtc cgatcagcgc cacctgcgca ttacctccac
     7141 gctgctgcgc gccatcaagg atatggacag ctataagtcg cgggaagttc tggagagctt
     7201 tgcgtccgtc aagcccgtgc cccagcgccg attctacacc gccatcgaac aactgagcgg
     7261 caaagacgga gagaaggccg gcgggttccg gaagtacggc aagcactacg ccaagtgcgc
     7321 gcaaggcgat accggcgcgc tggaggtcat cgcggacagc ggcactgtgt ccggcgctca
     7381 gatcaaggca accgatgtca cgcctgtggc cgaccctgca tacaagcccg ccgccggcga
     7441 ctatgtcaag gcggtggctg gcgcggagat caacttcatg atcctccaca ctccggcgct
     7501 catccagttc cagaagcacg tggccccgaa gatcatctct ccggagcaga accagaccgg
     7561 ggacgcctgg aaatacgggt accgaaatgt cggcattgcg gacgtctacg caaataagct
     7621 cgccggtgtg ttctgccatc ataaggccgc cggcgtctg
//
LOCUS       DAWBSB010000101         7542 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1958_length_7542_cov_3.207560, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000101 DAWBSB010000000
VERSION     DAWBSB010000101.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 7542)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7542)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7542
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1958_length_7542_cov_3.207560"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1851..1860
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1542 a         2181 c         2454 g         1355 t
ORIGIN      
        1 ttgtaatact gggtgcaggc ctcggtgctg atggtgaagc cctggggaac aggcatgccg
       61 gcattggtca tctcggcaag gttagcgccc ttgccgccga ggatctcacg catgtccttg
      121 ttcccttcac tgaacaggta caggtatttt ttgtcactca aatttctcta cctccaaatt
      181 tttttgtgca cgggaaagcc caaaacccat tcccgtgcac gaatgaacaa aaaacttgtc
      241 catttgcaca catcattata acaaaaagcg gcgggcaatg ctactgttat ttccctgttt
      301 tttattgtat tttaacgcgc aattttgaat ttttggtgca attttgccga tgcccttgca
      361 aaacgcccgg aatatcgtaa aatagaagtg tatagataag cgttatcgaa ctctgcggac
      421 gggccttgcg cgcgccgcac atcaaaccag aaacgggggc gtttgtccat ggagaacgaa
      481 gcaataacag cataccggca gcgccgggcg gcatttgagg caacgtttga gcggcacctg
      541 gccgcgggcg ttgtttttta ttccgccgac ggcatcctca tcgacgacac ggtagagatc
      601 gcgccgggcg cggtcatcct gccgggcacc atcctgcggg gggggacaaa aatcggcgcg
      661 ggcagcgtca tcggcccgaa cagcctgctg gaaaacgtga cggtgggcga gaacgtgaaa
      721 ttcaacgcca gccagggtta tgacagcgtc atcgagggcg gcgcggccat cggcccgttt
      781 gcacatattc ggccggacag ccgtgtgtgc gaaaatgtgc acatcggcga ttttgtggag
      841 atcaagaatt ccactgtggg caagggcacc agcgtttcgc acctgaccta tgtgggcgat
      901 tccgacgtgg gcaaatactg caatttcggc tgcggcgtgg tcaccgtcaa ttacgacggc
      961 gcggccaagc accgcaacac catcggggat tatgcgttca tcggctgcaa tacgaacctg
     1021 gtggcgcccg tgagcgtggg cgacggcgcg tacaccgcgg cgggcagcac cattaccgac
     1081 gacgtgcccg cgggcgcgct gggcattgcc cgggcgcggc aggtaaacaa gccgcagtgg
     1141 gcgaagggca agctggaaaa gttcatcgca aagcagcagg caaaggaaga agccggctcg
     1201 aaataaactt tgaaaacaga ataaggacag aatgacggat gatgcggaaa ggacgtgcgc
     1261 gggtatgaat cttcacggca aggatatcaa gatcttcgcg gggaattcca acaagccact
     1321 ggcggcggca atggcggagt gcctcggcat cacgctggga cgcagcgaag tgggacgctt
     1381 cagcgacggc gagatacagg tctccatcca ggaatcggtg cgcggcagcg acgtttatgt
     1441 ggtgcagagc acctcaagcc ctgtcaaccg caacctgatg gagctgctca tcatgatgga
     1501 cgctttccgg cgcgcgtcgg ccgggcgcat cacagccgtc attccgtatt acggctacgc
     1561 ccggcaggac cggaaatccc gcgcgcggga ccccattacg gccaagctgg tggcggacct
     1621 gatcgtaacg gcgggagccg accgcgtgct gaccatggac ctgcacgcgt cgcagatcca
     1681 gggctttttc gacgtaccgg tggaccatat gtacggcgcg cccattctga cgccctattt
     1741 cgcacgcaaa acggagcctt accatgacaa cgtgacggtc gtcagcccgg acctgggcag
     1801 cgtggcccgc gcccgtgcct ttgccgaacg gctggacgtt ccgctggcca nnnnnnnnnn
     1861 gcaaatgttt ctgaggtgat gaacatcatc ggcgaggtgg aaggccggga cgtggtcatc
     1921 gtggacgata tgatcgacac cgcgggcacg ctgtgcaatg cggccggcgc cattaaggcg
     1981 cgcggagcca agagcgtgac ggcctgcgcc acccacggcg tgctctccgg tccggccatc
     2041 gggcgcatcc gggacagcgc gctggatgag gtcgtgctgc tggacaccat cgccctgccg
     2101 ccggaaaaac aaattgaaaa gatcaaggtg ctcagcgtcg cgcccgtgct ggccgaggca
     2161 gtggcgcgca tctattccga caagcccgtc tccacgctgt ttgtgtgagc ggcgcggctg
     2221 aaataataca atacaaatgc agcggcacgg cgcggtgcgc cgtgccgccg tgttgtgcat
     2281 tccacaggag agaccatgtt tttcaaaaag cagacaagca ttgatacgat catcgtgggc
     2341 ctgggcaacc cgggcaaaaa atatgaaggc acgcgccata acgcgggctt cgccgcgctg
     2401 gaccatgtgg cggaaaaatg gggcgtgagg gtgacgaagg cgaagttcga cgcgctgacg
     2461 ggcacgggca cggcggcggg cgtgaaggtg ctgctgatga agccgcagac ctttatgaac
     2521 ctttccggcg atgccgtgcg caaggcggcg gatttttaca aggtcccgcc ggagcgcatc
     2581 atcgttctgt tcgacgatat ctcgctggcg ccgggacgcc tgcgcatccg gaaaacgggc
     2641 tcggcgggcg ggcacaacgg catcaaaagc atcattggga acatcgggca ggatttcccg
     2701 cgggtaaaga tcggcgtggg ggaaaagccg cgtccggagt acgacctggc ggactgggtg
     2761 ctgagccgac tgacgcaggc tgagaaaaag gccgtggagg cgcgttacga cgatatctgc
     2821 ggcgcgctgg agcttatgat gaaagggcag tttgacactg cgatgtcgag gtacaatgga
     2881 taagaaaaaa gagaaaatga cgccggagcg ccggtatgag cttgcctgcc tgattttggg
     2941 cggcgtggtg ctgggtggtg cgctgggctg cattttgcgg cccaacgaat ggacgtttgc
     3001 cgttatcccg ctgctgggcg tgtgcctgac ggcgcttttg tggtgcgcgg cggcgcggct
     3061 gtggctgtgc cgcgacaagc agcgcctgct ggcaaaaagc tttacgcggc ccgtgcggtt
     3121 cgcgctggcg gtgcctgcct gggccggcgc tacatggctt ttgtatctgc tgccgggcaa
     3181 tgtgacgggc ctggacgacg tgctggcgga gagcgttatc atcttgacgc ttctggccgg
     3241 caccgtgttc gtgcccgcgg ctgcgctggg cctgggtgtg gcttccgttc tggcggccgt
     3301 ggtgcatctg cgccgtacgc ctgccgctga gacgcgggcc taccgcgagg ggccgttggc
     3361 gctggcactc gttttgtgcg tgggcgtggc ggcgctgtgc gtatgttttt ttctttattt
     3421 gacgatatag ccgcctttgg cgcagagagg ttttttcatg ctgactgctc ccctgaaaca
     3481 aacacctgaa tacaaacggc tgctggcggg catggcgggc gcgaagcccg ctgtggtggc
     3541 cctgttcggt atgccgccca cggcccgcgc ccagatgctg gccgcactgt gcgaggatac
     3601 gggccgttcc gcgctggtgg tgtgcgcggg cgaggccgac gccacgcgct ttgcggagga
     3661 cgccgccgtg ttcggccggc gggccgaggt attccccgcg cgggattatg tgctgcgccc
     3721 cgtggaaggc cagagccatg aatacgagta ccggcgcctt gccgtgctgg gcaacctggt
     3781 gggcggccgc acgagcgtcg cgtgcgcgcc cgtggaggcc gtgctgcaat atacaacgcc
     3841 caaacaggaa ttctgcggca acactctcac ggtgaagcag ggaatggcta tctccatgga
     3901 cgtgctcatc gagcggctgt tcggcgcggg gtatctgcgc cgcttccagg tggacggccc
     3961 gggccagttt tcggtgcgcg gcggtattgt ggatatttac gcgcccgaca tgcagaagcc
     4021 ccaccgcctg gagttttggg gcgacgaggt ggacagcatc cacagctttg accttgtttc
     4081 ccagcggcgg gaaggggcgg tgaaaaaaat ttatctttcc ccggcccggg aagtgctgtt
     4141 cggagacacc gaggccgccg caaggctgct gcgcacggcg ctttccaagg caaagcccgc
     4201 ctacgcggaa gctttggccg acgccatgga cggcgacctc aaggcgctgg acgcgggtac
     4261 gatgcccgcc gccatggaca aatatctggc gctgcggtac gaaaagccgg cgacgatctt
     4321 tgaatatttc gacgcgccca tcgtgttcct caacgagcca agcgccgtgc gcgaggccgc
     4381 caaaggcgtg gaattccgct tcggagaggc gttcaagggc ctgttggaag agggtgtgct
     4441 ggcccccggg ctggaccgtt tctacgagga caccgcgtat ctgctgcacg agacggagaa
     4501 gcggcatgcc gtggtgtgtg aaaattttgc ccgcaccatc ccggacctga aactggcgga
     4561 taccgtcaac gcccagaccc acagcctgca caaaagtatg ccgtggcagt gctggcgggc
     4621 acgcagcgcg cggccgccgc gcttgcgcgg gaccttgccg ccgcgggctt ttccgcccag
     4681 gcgtatcata aggaaccgcc gtcccttgcg ccgggcagcg tgggtgtgtt tgaaggccac
     4741 ctgaacgcgg gcgcggatta ccccttcgcc aagttcgccg tgttcaccag ccgccgccac
     4801 aacgcggccg cgccgcagaa ggccagggca aagagcaagg gcctttccag cctgtcggac
     4861 atcacgcccg gcgattatgt ggtgcaccag aaccacggca tcggggtata tgcgggcatc
     4921 cagaggctgg acctgcaggg cgtggtgaag gattacatca aaatcaacta cgacaaagcg
     4981 gacacgctgt atgtgcccgt tacccaactg gaccttatca gccgctatac agctcccggc
     5041 gacggcgagc gcgtcaagct ctcgaagctg ggcggcgacg cgtggaacaa aaccaaaagc
     5101 cgcgtgcgca aggccacgca ggagatggca aaagagctga tcgctttgta tgcccggcgc
     5161 cagcaggccc agggctatgc gttccccgag gacaacgagt ggcagcggga ttttgaaacg
     5221 cgttttgaat acgacgaaac ggccgaccag ctcaccagcg cggccgaaat aaaaaaagac
     5281 atggaaaagc cccacccgat ggaccgcctg ctgtgcggcg acgtgggcgt gggcaaaacg
     5341 gaggtggcgc tgcgcgccgc gttcaagtgc atcatgggcg gcaagcaggt ggccgtgctg
     5401 gtgcccacca caatcctggc gtggcagcac tacaccacca ttctctcccg catggaggcg
     5461 ttccccgtca aagcggggct gctctcccgc tttgccagcg cccgccagca gaaagagacc
     5521 attaagggcc ttgccgcggg tactgtggat gtggtggtgg gcacccaccg tatgctgcag
     5581 aaggatgtga aatttcacga tctgggcctt gtcatcatcg acgaggagca gcgcttcggc
     5641 gtcaagcaca aggaaaagct gaaggaaaat ttcatcggcg tggacatgct gacgctttcg
     5701 gctacgccca tcccgcgcac gctgaacatg gcgatgagcg gcctgcggga catgagcacc
     5761 atcgaagagc cgccctttga gcgccgcccc atcgagacct atgtgatgga atacgacgcg
     5821 ggcgtggtgg ccgaggccct gcggcgcgag ctggaccgcg gcggccaggc gtattatatc
     5881 cacaaccgca tcgataccat cgaccagtgt gcggcaaagc tggcggagct tttgcccggc
     5941 gcgcgcatcg ccacggccca cggccgtatg gacgaggcca cgctttcggg ggtatggcag
     6001 aagctgctgg acggcgagat cgatgtcctg gtgtgcacca cgctcatcga gacaggcgtg
     6061 gacgtgcgca actgcaacac gctcatcata gaggacgcgg accgcatggg tcttgcccag
     6121 ctgtaccaga tccgcggccg cgtgggccgc tcgggccgca aggcctacgc ctatttcacg
     6181 ttccggcggg ataaagtgct cacggacatt gcggccaagc gtctttccgc catccgggag
     6241 ttcacaagct tcggctccgg cttccgcatc gccatgcgcg atctgcagat ccgcggcgcg
     6301 ggcaacctgt tggggcagag ccagcacggc cacatggagg ctgtgggcta tgagatgtat
     6361 gtcaagctgc tgaaccaggc catcgccgcc gaaaagggag aggctgttca ggcggataag
     6421 agcgattgcc tcattgacat caccgtggac gcctacatcc ctgaaaaata catcccggac
     6481 gccgccggac gcattgaggc gtacaagcgc attgccgcca tcgatgaccg ccccggcgcg
     6541 gacgacgtgc tggacgagct ggcggaccgt tacggtcctg tgcccaaaag cgtgcagggg
     6601 cttatcgaca tcagcctggt gcgggtggtg gctgcaaagc ttggcatcta cgagatcaca
     6661 caaaaccggg atacgctcat tctgtattcc gataagctgg acctcgccac gctcaagccc
     6721 ctgctcaagg atatgggccg ccgcctgctg gtgaacgctt ccggcaagcc gtacctttcg
     6781 gtgcgcatcc tgcccggcga aaagccgctg gacgtgatga atgccgtgtt cgacaaaatg
     6841 gcggagctgg agcggccgca gtaacgcgct ccggggccga aagttttgcc cggcacttgc
     6901 caacagccgc gcaacgcggt ataatttgtt gtatcattgc gggtgaaggc cgcggccgaa
     6961 gcggcgcccg cgcggaggga aacacattga agctgaaaaa attcaccggc ctgctgctgt
     7021 gcgcggcgtt ggccctttcc atggctgcct gtaagttcac cacgcccgcc gtcgtgatga
     7081 cggtggaggg cgaggatatc cccgcgggcc tgtacctgat gtaccagtat caggcgtatt
     7141 ccagcgcaaa aagcaagctg gaggataaaa gcgccaaggt actgaaaagc gagatcgagg
     7201 gcgtgaaagc tgaggaatgg atccacaacg agacggtggc ctccgcaaaa cgttatgtct
     7261 gggtggaaaa agcctttgcg gaagcgggcc ttaccttcac cgaggaggag caggcgtcca
     7321 tcgacagcca gctcgatacc atctgggcca acaacgaagc cctgctggcg gccaacggca
     7381 ttgggcggga aaactaccgc cgcttctatg aatgcgaagc gaagtatgaa aagctgctgg
     7441 ctgagtatca ggacggcgaa tcggacaaga tcaccgacgc cgaggccaaa aagtacatgg
     7501 acgagaccta ttcccgcatc cagacgctgg tgctgccaac ta
//
LOCUS       DAWBSB010000102         7514 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1966_length_7514_cov_3.867274, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000102 DAWBSB010000000
VERSION     DAWBSB010000102.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 7514)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7514)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7514
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1966_length_7514_cov_3.867274"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2191..2200
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1274 a         2260 c         2576 g         1394 t
ORIGIN      
        1 cgagggtgcg gccggcgtgg gacgccggcc gcagctgctg gccatcaacg ggccgggccg
       61 attttcggca gtgttcgcgc tggaaggttc gtttctctcc atggggctgg tggacatctc
      121 cggccgtgtg ctccacaggc agaaaacacg cacggcgcag gatttcggcg cgtttctggc
      181 cgaggtgcgc gacgggctgg tgagcagcct gctgcacgct gccggagcag accctgcacg
      241 gctgatcggc gtgggcgtca cgctgcccgg gacgtatgac cccgcgcgcc gcgtgctgga
      301 ttacgcgccc gccatcggtg tgttcgggcc ggtggagatc ggcccgtgga tggacgggat
      361 gacgcagaag ttcggcgtgc ccgtttatgt ggaaaacgac accaacgccc agtgcatggg
      421 cgagcatgag ctttgccgtg cgcgggacct tgtgtatgtt tcggcgggaa cgggcgtggg
      481 tgcgggggtc atgctggacg gccgcctgcg acggggcgaa acgaacaaat gcggcgagat
      541 cggctacaat gtgtttttcg aggattataa gcgcacggcc cgcgcagcgg gctgcctgga
      601 gcagcgcatc ggttaccatg tgctggagca gcagttcgcc ctggaccgtt tttcccgccg
      661 cagccagatc ccgcccgcgc ttctggcgca gatggcggcc caggtgacga agcccctggc
      721 gctttgcatc gccaacctga cgcatgtgct ggactgcagc accgtggtgc tgggcggcga
      781 ggtagccgag ctgctgggcg acgcgcttct ggacgcgctg aacgccaggc tggaggagct
      841 gtgcctctcg ccggtgcggg tgcgccgtcc ggccagcgcg gacgacggcc tcatcggcat
      901 ggcggcccat atcacgcgca tggaagtgga tgcgcttttg gaggaggaat aggctgtggc
      961 atatttaaag tttccgctgt ttctggggcg atacgtcaac cgctggctcg tcagcggcat
     1021 tgcccagacg cccgtgcatt tcacaccggt gacgctgcac ggggatatca acctgtggct
     1081 gaaaaaggga ttttccgttc atgaaaatcc ctgcaagaca gaattcgtac gcgcgcgccg
     1141 ggcacgcccc gccgcgctgc ccgctgtatg cgagccggtg cccggcggcc gcgttggaga
     1201 cggcgcgtcg ccccagacgt ttgccgtgta ctggcccttc gacgacatca gtcttgacat
     1261 ctccggcgga tgggacgcgc ccacgcatat ccgcgcctgg gcgtataccg agctttgggc
     1321 ggacgaggac ggccccgcgc cgttcctctt tacgacctgc ggcggagcag ccgtgtggct
     1381 gaacggcgag aaggtgctgg aatttacgcc cttcacccgc aacatccccg cggatacgcc
     1441 gctggagctg acgctgcgca agggcagaaa cagcgtgctg gtgttttttg atgacctggc
     1501 cgagcgggac gcggcgttcc tgctgcgttt gtgctggcag ggaacggatg cgccgccgga
     1561 gcagcgcgtg ccggtgggcg cggcgaaccc tacgctgctg gagcagggcg agcaggccat
     1621 gcgcagcctt tgcttcagcc gcaaccatta tgcggcgggc cccgtcagtc tgcggtgtga
     1681 aaatcctttc gcccagcaga cgctgcatgt aacgctggag ggcgctacgg aggaaaacga
     1741 gcaggcgggc gtgctgttca cgcgcacagc ggattttgcc cccgggcaga cacgggcgtc
     1801 cctgggggac tgcgccgagt tcccgttcgg atttcttctg ctgcaggcca ccgctgtggt
     1861 ggagggaata gccatcaccc gccccatcac cgtggaaacg cacgcctcgg ctctgctgcc
     1921 ccatgcggcg gacaccgtgg ccgggcgcaa gcggcaggcg ctggaattcc tggcgcactt
     1981 cggcgagcag aacaccaacc gggccgtggc gctgctggca acgggcggca gcccggacga
     2041 ggcgcagcgc ctcattgccg cgcaggtgcg ctttgtagac cgccggtgcg actgcagtga
     2101 tttttacctc gtttattttc cgcatatcct gcgggcctgg ggcgcacagg gcgccggcct
     2161 gctctcgccg gagctggagc gcgccatgcg nnnnnnnnnn catgcgggcg tgcatcctga
     2221 acttccgtta ctggatggat gaaccgggcg atgacgtgat gtggttttac agtgagaacc
     2281 acgcgctgat gtttcatacc tgtcagctgc tggccgggga gctgtatccg tcggagacct
     2341 tttccaacag cggcatgacg ggcctacaga tgcagcagaa agcgaagggc ctgctgctgg
     2401 aatggttccg gggattttta aacgagggct ttacggaatg gaactccagc gcttacctgc
     2461 ccatcgacct gctgggcctg gccagcctct atgcctggac gcaggacgga gagctgcggg
     2521 cactggcaaa acggggcatg gactatgtgt tttatctgct ggccgtacac agccgaaaag
     2581 gctttttcgc agggtcctcc ggacgcacgt acctcaagga gcagttcgga aactggtcca
     2641 actgcacatc gttcatgagc tggatcggct atggctgcgg cactccgggc cacgcgggca
     2701 agggcgtggt ttccctttgc ctgtcggatt acgagccgcc gcgggactat gcggcctatt
     2761 tcaatgtaga gccgggtttt gagcttgtgt gccgctccac gcatggttat cagaagcacg
     2821 tagatcttta tacctataaa acgtcgggtt atctgatgac aagcgcggcg gatttccggc
     2881 ccggaaagcc gggctatcag gaaaatccgc tgcagctcac attcacaccc acggcgcagc
     2941 tctggatcag ccatccgggt gagcgggcgc tgtacggcaa ggggcgtccg tcctattggg
     3001 cgggcaacgg cacgctgccc cgtgtcaacc agtacaaagg cttcgccacg gtgttttatg
     3061 atatcgcgcc ggaacacccg gtggatttca cgcatctgta tctgcccaca atggaatttt
     3121 atctgtgccg cgtacagggt ccctgggtgt ttgcgcagga gggagacgcc tactgtgcgg
     3181 tctattgctc ggccgggctc acggcccagc gcttcggccc aaacgccgag cgtgagttca
     3241 ttgcgcccgg ccgccgctgc gtgtggctgg tgcgcgccgc gcagacggat gaattcccca
     3301 gctttgcggc gttcatcacg gccatgctgg ccgcgccgct ggaggtggac gcccagcggc
     3361 ttgcctgccg ctttgaggac ccggtatacg gagtgctgcg cagcggctgg aacgaacgcc
     3421 tttcggtgaa cggcgcggca atggaatacg gcggcagcga ccagtacggc gtgctggagc
     3481 tgcgggaaaa acaacagcct gccgcggatg cgcaggcata ggaggaaaga atatgctgga
     3541 ccagaagacc ctgcacgcgg cgctcaaaaa ggtatcggcc gcgatgatgc atctggaaaa
     3601 tacgggcttt gaggaaaagt acccaatcgg cctcatcgac atcaacctgt gggaatggcc
     3661 gcagggcgtg ggcctgtacg gcctttacaa ttattataag ctgacgggag accgcgggac
     3721 gttcgatttc ctgtgcggct ggtatgacgc gcgcatggcc gagggcctac ccgagcgcaa
     3781 cgtcaacacg acctgcccca tgctcacgct gctgtgcgtg tacgaggaca cacaggacga
     3841 acgctacggc gcgctgtgcc gggattgggc cgagtgggtg atgcacgggc tcatccgcac
     3901 gggcgacggc gcgtttcagc acatgatcac cggcgacgcc aacgacagcc agatgcttat
     3961 cgacacactg ttcatgacgg tgctgttcct ggccaaagca ggcgtggtgt tcggccgcaa
     4021 ggactatgtg gaggaagcaa agcgccagtg cctggtccac atcaagtatc tgtatgacac
     4081 aagctgcggg ctgtatttcc atggatacga cttcaaggga cgccacaact acggccgtgt
     4141 gcattgggca cgcggaaact cctggtacac ctgcggcatc atggagctga tcggccttat
     4201 ccccatcgag ccggggctgc ggcagtattt cctcgacacc ttccgcgccc aggtggacgc
     4261 gctgtgcgcc tgccaggcgg aggacggcat gtggcatacg atcttggacg acccggcgtc
     4321 ttataaggaa agctcggcca cggcggcgtt tgcctacgga atgctcaagg gcgtccgtct
     4381 gggctttttg cccgcgcagt atgaggcgcc ggccctgcgc gccgtgcagg ccgtgctggc
     4441 ccgcgtggat gccgcgggca tcgtgggcgg cgtttcctac ggcacgcctg tgggcaatga
     4501 cgcacagttt tataaggata tccccgtctg tcccatgaca tacgggcagg ccatggccat
     4561 cctgatgctg gccgaggcgc tgtaccacac cggggaggac gcgtgatgga aacaggcgcg
     4621 aacgtccctc tgtatgggcc gtattccaaa aaatatctgg gcctttccaa aatcgttccg
     4681 cgggcgcagg tgccgggcgc gcggttcgac cttgtcgttc accccacctg cgtgaacggt
     4741 gcggtgcccg ttccaaacac cacgtttccc tgtgggtatc atccgtggca ggccgcgccg
     4801 gacggctcgt tttacagcta ccgctgcgag ctgctgccca aggacaggct gtacgcgagc
     4861 attgcgtttt ttcgcctgga tgaaaacgcg tggggcgtgc gcacgcggtt ccacaaccgg
     4921 acagagctgc cccgaaattg tatgctcaac tatttctgcg cgctggaata cccggctgcg
     4981 cggcggacgc tgcccgttct gccggagggc gcggtccgct ggcatgcgct ggactatgct
     5041 tcctatactt acgcgcagcc gcgcccgtgg gacggccttg cgcccgacgc gctccaaaaa
     5101 ggtgagttcc gcgacgaggc gttcacagac ggccacggcc tgggcgaccg cgtgcctcac
     5161 gcccacatgc cgcgccttgc gggcctgcgt ccctttggcg cgcaggccgg ggatgctgtg
     5221 ggctaccgcg tcgccgttcc cgggtttgag gatgccgcgc tcacggtgcg gtaccgtacc
     5281 gtaccgccgc agggcgggca cggggaaagg ggcgcttcgg cggagccggt ggtgttcgat
     5341 actgcgttcg gtccgctgtc ctttccgccc agcggcgcgc ccgcgtttgt ttcgttcccg
     5401 ctgggcgcgg tgccgccggg ggagcttgcg ctcacgctga cggcgcgcgg taccggcggc
     5461 gcgggcgtgg agctggactt ttttgcggtg acggcccgcg cccgggcggc tgccgtcggt
     5521 gtgtgcacac aggagccgga tgccaggccc gtattggaac gggaaaggga cgggcagtac
     5581 cgcctgcgct atgccggtgt ggacgaggcg ttctgtgtgc gcgtatacgg cgaaaggcgg
     5641 cgggagcgcc tgctggagac cggctctctg gaggatgcgc tcatctcccg cctgtccaac
     5701 ccggaccata ccttcgacga tgtgctgaac ccgttttccg gttcgttccg gcgcaagcac
     5761 agtgacccgg gctggtatca aaacctgctg gtgagcgggc tgttcctgca gcccggggcc
     5821 tgccatacag aatacgccgt gctctcggcc acgccccgga cggacacgcc gcaggcactg
     5881 gagcaggtgt tccgcgcggg caagcgccgg gcaaagctgc cggccttcaa cccggcgggc
     5941 aggcgctacc ggctgtcggt gcggtgcctg cgcgccgccg cgctgacgaa tgtcgtttat
     6001 cctttgtacc gcaggggtga aatggtggcg cattatacgc cgggaaagcg gtgggattct
     6061 ttctacacat gggactccgg cttcatcggc ctgggcctgg cacaggcgga cgcagagctg
     6121 ggccgccgcg tgctggagca gtatctttcc ggcccggaaa acagcgattt tgcctttgtg
     6181 caccacggct cgctggttcc ggtgcagttt tacctgtacc aggacctgct ggcgcgcgcg
     6241 caggataagg ccgggctgct gcggctgtat ccggcgatgc ggcggtatta cgaatttttg
     6301 gccggacggg gcgaaggctc cacgaccgcg cgctttgcaa gcggcctgct gacgaattac
     6361 gattatttct acaacgccag cggaatggac gactatgcgg cacaggtgct gatgcatgcg
     6421 aagggccttt cgggccgcgc cgcacccgtc ctgttcaccg cccatgtcat ccgcgcggcg
     6481 aagatcctgc gccagagcgc gcggcgcctg gggctcgtga aggacgagga gcgctgcggg
     6541 cgggacatcg agcggctttc cgccgcgctg cagtgcgcct gggacgggga atgcggctac
     6601 ttcagctatg tgctgcacga tgacgaagga aggccgcagg gctttctgcg cacggacgcg
     6661 ggcgagaacg caaacaaggg gctggacggt gtttatccgc tggtggcggg cgcatgtacg
     6721 gcacggcagc aaagcacggc gctggcgcat ctgaaaagca cgaaggagct gttctcaccc
     6781 gttgggctca cagccgtgga catgtccgcg tcttattacc gggatgacgg ctattggaac
     6841 ggcgcggtgt ggttctcgca ccagtggttt ttctggaaaa ccatgctgga cctgggcgag
     6901 ggcgcgttcg catgggacat cgcgcgcacg gcgctggagg catggaagcg ggaggtggac
     6961 gacagctacg acacctttga aatgatcaac atcgccacgg ggcgcggggg ctggttccac
     7021 cagttcggcg ggctgtcgtg tccggtgtat ctgtgggcgt gcgcgtattt ccagcccggc
     7081 acggtgaact gcggttttga cgtgtggctg gagcgcgcat cctttacaaa gaactgtacg
     7141 gcctgcacgc tggaactttc cacccgggat tgtgcgcggg atgcgctggt gctggcggcg
     7201 ctgtgcccca gcacacgcgt gcgcgccgtt ttgggcggca cagtgctgcc ctgcaccgag
     7261 cgcgcgcccg gcgcgtggga ggtcacggtg ccgcacgggc tgaaaaacgc cgtggtggag
     7321 ctgcggccgg aatgacccct gcatacgcgg tgtgaagctt ccgtgcagaa caaggccccg
     7381 cccgtccgca tctgctgcgg acgggcgggg ccttccaggt cgtcgaaaaa aagtaaagaa
     7441 gtaaaagaag agtccgggga aaaagctggc ggtggcaaag ccatgcatct ttgcgcagca
     7501 aagtgcaggc cacg
//
LOCUS       DAWBSB010000103         7472 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1979_length_7472_cov_3.714979, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000103 DAWBSB010000000
VERSION     DAWBSB010000103.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 7472)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7472)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7472
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1979_length_7472_cov_3.714979"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1238 a         2354 c         2572 g         1308 t
ORIGIN      
        1 ccgttatacg cggcccgagg gcggcatgtt catctggggc acgctgcccg aaggagcgga
       61 tatgcccgcg tttgtgcggg aatgccttgc gcgcaagctg gcgcttgtgc cgggcaacgc
      121 gttcatggtg gacgaagaag cgccgtgcgg cagcttccgc atgaattttt ccacccccac
      181 gccggagaac atcgtgaagg gtgtttccgt tatggccggg gtgcttgccg cgcaggggct
      241 ctgacatgca ggcgggataa aaacaaacca gagcggcgct ccctttcaaa gagggagcgc
      301 cgctctggtt ttctgcatca gccggagcgc acggctcaca gcgccagctc cacgccgcgg
      361 cccgtgcgct tgtacggcgt gtagcggatg cccaccatgt cgaacatccg tttgctggcg
      421 atcacggaat cggaatccgc gtatttgtcc tcgtaataga tgacctgtga gatgccgctc
      481 tggatcagcg ccttggcgca ttcgttgcag gggaacagcg tggtgtagat gcgcgcgccc
      541 ttcaggctgc ctgtgccgga ggcatggttc agtatggcgt tcagctcggc gtggcagaca
      601 tacatgtatt tggttttcag cggttcgccc tcgcgctccc agggcatgtc gtcgtcgaag
      661 cagcctgtgg gcatgccgtt gtagcccatg gacaaaattt tgttttcgcg gctgacgatg
      721 cacgcgccca cctgtgaggt gttgtccttg ctgcgctgcg cggtgagcag cgccacgccc
      781 ataaaatatt cgtcccagga aatataatcc gtccgtttca aagcagggcc tccttgatct
      841 gtgtatgatg tttgcgcggg aacaccgcgc gtcaatccgt catggggctg acgccgtcca
      901 gatagtccac ggcggccagc gcggccacaa ggccgtcgcc cacggcgttg gccacctgca
      961 gcggcttgcc cgtcatgtcg cccgcggcga acaggccggg aatgttggtg cgcatggcgg
     1021 cgtctgtttt tacaaagccg ttttccagcg caaggccggg caccagcgcg tccggcggcg
     1081 tggccgcgcg caggataaaa acgccctgta cgggcagcgc ggcgccgccc acgcgcacgg
     1141 cctccacggc ggccccgccc tccacggcct ccacgtgccc ggcccggaag gccacgcccg
     1201 cttccagcgt gtcgggcctg cggcccgcga tgtaggtgac ggagcagccg atgccgtgca
     1261 ggaaattggc ctcctgcggc gcttcggggg aaagccccca caccgctacc gcgcgcccgc
     1321 ggtacagcat gccgtcgcag gtggcgcagt aggaaacgcc gcggcccagc agctcggcct
     1381 cgccgggcac gggctttgcc ttggaaacgc cgcaggccag caccacggcg cccgtttcca
     1441 ggatatcgcc gtccaggttg accatgaagc gtccgccgaa cggcatgacg ttgcccgcgc
     1501 gccccttgcg cagcacagcg cccgcgtccc gggcgtgctg cgtaaagcgc tccatcatct
     1561 gtttgccgga cagccccggc atgcccagat agttgtccac ctgctcggcc ttggccagca
     1621 gcgtttcgcc gctgtgcacc accagcacgg tcttgccgcg ctggcgcagg ttcagcgccg
     1681 ccgaaagccc cgctgggcct gcgccgatga caatgcagtc aaaattcgtt tccatttgta
     1741 ctccttatcc ggtgtggtca cagcgccaca acgagcgtgc cgacggtgat gagcacgccg
     1801 cccagcagcg ttttgagcgt gagcttttcc cccaggaaaa gcgcggccag aagaaatgtg
     1861 aggatgaggc tgaatttgtc gatggtgacg acctttgcca cctgccccac ctgcagcgcc
     1921 ttataatagc acagccagct tgcccctgtg gcaaggccgg agagcatgag gaacacaagg
     1981 cttctgccgc cgatgtcctt catgccggac tgcgccccgg tgatgaacac catgccccag
     2041 gccatcacaa gcacgaagat ggtgcggatg gccgtggcca ggttggaatt gatgccctgg
     2101 acgccgatct tgccgaggat cgccgtcagg ctggcgaaca atgcggaaag ccccgcgtaa
     2161 acggcccaca tgaaaaagcc cccatttctt tgtgcgggcg tgcccggccg caccgggcgc
     2221 ctttgcgttt attatacagc agcacggggc aaaaacacaa gcggccacgg ccttttcggg
     2281 cccttccgcg cgtgtttttt tgccgccggg cgggaaagcg cccgcgccgg cggggcaggg
     2341 ggcttccgca aatgcggccg ccgggcgcgt gcacccaagg gcgtgccttc acgcgccgcc
     2401 gggcccgccg ggccggggcc tattccacat atgcggggac ggcgttttcc ggcatggggc
     2461 aggtgtagcc gtccgggcag ctttgcgcgt gctgctccct ggccagggcc agaagcgcgg
     2521 ggtccgctgc cgcgcgcgcg cccgccagcg ccatggctgc cgccgccgcc agcatgccct
     2581 tgaagcccag cgggctgcag ctctgcgcgg ccatctgcca ggtgtggccg cccgtgccca
     2641 gcgccccggc ggcgtagtag cactgagccg tgggcgcgca gtagctcacg tcgcccacgt
     2701 cggtgctgcc gggctcggcg gttttggggt cgtaaaagaa cggcgcgatg ccgtcgtcca
     2761 ggtccttgcc cgcataatgg gacagcggca ggtccgtggc cgagagcgtg gaggccaggt
     2821 tgtccgcgcg atccccggcg gagagcgtgg cggcgaactg tgcggccagc gcccggtcgg
     2881 cttcgtccca ggcgggcgcg cccacctccc gcatgcattg ccacagcagc tcggacagcg
     2941 ccctgttggg cacatagtcg gagaagccgt ccacctcgcg gatggtgagc gccgtgcccg
     3001 tcatcagcgc ggccccccgg gccacatcgt ccacacgccg cttgatgtcc agcatctggg
     3061 ccacacgggg cgcgcggatg tagtaatgca cgcaggcgct ctcctgcacc acgttggggg
     3121 cggcgccgcc cacgtcgcgg taagcgtaat gcagccgcgc ctcggggatg atgtgttccc
     3181 gcagatagtt gcagcccacg ttcatcagct cggccgcgtc cagcgcgctg cgcccggcaa
     3241 agggcgcgcc cgccgcgtgg gccgtgcgtc ccttaaagct gtacagcacg cccgtcacgg
     3301 caaggcagcc ctgcccgctg acgtagttcg cggggccggg atgccaggcg aacaccgcat
     3361 ccaccttgtc gaaatatccg gcgcgggcga aaaacacctt tccgccgccg tcctcctcag
     3421 ccgggcagcc gaagtatttg acggtggcgc gcaggccggt ttcgcggcag tagtccttca
     3481 gcgccacggc tgcggcaaag ctgcccgcgc ccagcaggtt gtgcccgcag ccatggcctg
     3541 ccgcgccctc gcaaagcggc gctttttcag cgcggcccgc ctgctgggaa agcgcgggca
     3601 gcgcgtcgta ttcgcccaaa aaggcgatga ccggcccttc gccgccccat gtggcgacga
     3661 aagccgtctc catgccgcag gggcggcgct ccagcgcaaa gccctcggct tccagcgcgg
     3721 cgctgatggc ggcggcgctc tgcgtttcgc gccagttcgg ctccgcaaag ccccagacgg
     3781 ccctggccag cgcaatggcg ctttcctttt tttgttccac ccattcggtg atgcgttgtg
     3841 cttcattcat gtttttcacg tcctttgttc cgggcggcgc gctgtgcgcc gcggtatgcc
     3901 ttgtgcctgc aaaaacagcc aaaagctatt tcttagaata ccacattttg ccgcggaagg
     3961 gaacagccgc gcaaagcggc cgcccgcggt gcgccgggaa ggccgcgcgg ccgggcggaa
     4021 aaatatcccg cgccggggcg ctgttcaaag tttagagata aatgcgtggt agaataataa
     4081 aggaaggaag catcacacgg caggcacgcc ccgctgcgcg gggcgttggg aaaggcggcg
     4141 ggccatgaag gagctgtatc tcgtaacagg cgcgacgggc catctgggca ataccgttgc
     4201 gcacaggctg gcgcgcgcgg gcaggcaggt gcgcgcgctc gttctgccgg gggaggagca
     4261 cgcgccccag ctccccgagg gcgtggaggc tgtggcgggc gacgtgcgca gcccggcctc
     4321 gctggaggcg ctgttcgcgg gcgcggaggg gcgggacgtg acggtcatcc actgcgcggg
     4381 catcgtgagc atcgcctccc ggttcgacca gaacgtttat gacgtaaatg tgacgggcac
     4441 aaaaaacgtg gtggacgcct gcgtgcgccg cggcgtgaaa aagctggtgc atgtaagctc
     4501 ggtgcacgcg ctgccggaaa agcccggcgg cgcgctggtg tcggagacgg acgcgttcag
     4561 ccccggcgcg gtggttgggc tgtacgccaa aacaaaggcc gcggccacgg cctatgcgct
     4621 ggcggcccgc gcgcgggggc tggacgtccg cgtcgtgcat ccctcgggca tctgcggccc
     4681 gtatgactac ggccatggac acctgacgca gctggtgcag gatttctgca cgggccgtct
     4741 ggccgcgggc gtggacggcg gttatgactt tgtggacgtg cgcgacgtgg cggcggggat
     4801 actggcctgc tgcgagcggg ggcggccggg ggagtgctat atcctttccg gccggtactg
     4861 cacggtgcgg gacgtgctgg ccatgctgca ccgcatcacg ggccgcaggc gggtgcgggt
     4921 gatgctgccg ctgtggctgg ccagggctat ggcgcctctt tccgagcagt attacaggct
     4981 gctgcgccag ccgccgctgt acacggcgta ttcgctgtac acgctttcgt cgggcgcgcg
     5041 cttttcccat gcgaaggccg cgcgggagct gggctatacc acgcgcccct tcggcacgac
     5101 gctggccgac accgtggcgt ggctgcggcg gcaggggcgc atttgagggg cgcggctgta
     5161 aagttttttt cctgccgtga aaagccggaa aaagggcggc gggccacgcc gcaggggggg
     5221 ataagcggag caggagcgcc gtttatcccc ccttgattta tttttaagaa tttcttaaaa
     5281 ttgtgtagtt gatttggtgt gtcaaatcgt cttatagata gagatgaaag ccctgcggaa
     5341 gagcgggcgc tcaatgggga gttgtgcggc atgcgtgttt gtatcgtgat cccggcgtac
     5401 gagccgggcg gggagttcgt tccctatgcg caggaggtgc tggcggcggg ccttggcccg
     5461 gtgcttgtag tggacgacgg cagcgggccg gcgtatgcgc cggtattcgc ggcgctggcg
     5521 gagatgggct gcacggtgct ggcgcacgaa cagaaccggg ggaagggcgc ggccctgaaa
     5581 acggctctgc gctggtacgg cgcgcatgag gacggctgcg cgggcatcgt gacggcggac
     5641 tgtgacggcc agcatacggt aaaggatgtg cgccgcgtgt gcgaggccat ggaggcctgc
     5701 ccgggcacgc tggtgctggg ctgccgtgat ttcggccccg gcacgcccgc gcgcagcgca
     5761 acaggcaacc gcgttacctc ggcggccatg cgcgtgctgt acaacattga tttgaaagat
     5821 acgcagacgg gcctgcgcgg cattccgaac gggatgcaca gggacctgct ggaggtgcgc
     5881 ggcgaacgct acgaatacga gctgaacatg ctcatctacg caaagcagcg cagcatcccg
     5941 tataccatcg tgcccatcga gacggtgtat ttcaacaaca acgagggcag ccattaccgc
     6001 accgtggcgg attccgcgcg catcatccac cagctgggca gcgggctggt gcagtacgcc
     6061 atgtccgccg ggctgagcgt ggtggtggac gtgtttgtat actgcgtgct ggtgaaatgg
     6121 ctgctgctgg gccttccgct ggcgccgcgc ctgttttttg cggcggtcat cgcgcgcacg
     6181 ctctccagcg tggtgaacta tacctgcaac cgccgtctgc cctatgtgca gaacaagaag
     6241 atcggcccga cggtggcaaa atactactgt ctgtggctgg cgcagctgat gctgtccttt
     6301 gtgggcgtgt gggcggcgtg caccgggctg cacatggacg acatgctggc caagctgctg
     6361 gtggacgcgc ttctggccgt ggccagctac caggtgcagc ttcgctgggt gttcagtgaa
     6421 aagacgccgc ggccggtgct ttacggaagc ggcgaagcca tggccgcgaa gcggacaacc
     6481 gcacagggtg gtttcgcgca gcgaaatgcc gcgcctgatt ttgcgcggca aacccaaaag
     6541 gcggggccgg taaccggcca gcgcggtttc gccgcaggcg aaatggaggc ggaagccgac
     6601 aagtgcccgc agcacgcggc gggccgggag gacggcggca ggggggcggc gctgtgaata
     6661 aaaagaagat atacgggccg ctggcgcgct ttttacgcgc cggcgtccgt ccgtttttgc
     6721 acagatggga tacctccggc gcgcggaaag cgcagccgcc cgccgtgtat gtggtgcatc
     6781 accgcaatat gtccggccct gtgcatacgc tggccctgct gccggacgag ccgcgcccct
     6841 gggtgctgca tgtttttctg gacaggcacg aatgctttgc ccagtattac ggctacacgt
     6901 tccgcaaacg gttcggctgg cccgctccgg cagcctgggc ggcggcgggg gtgctttcac
     6961 tggccgtgcc cgcggtggtg cgctctttcg gagccatccc ggtctaccgc agcctgaagg
     7021 aaacgcggga catgatggag caaagcgtgc aggcgctgct gcgcggtgag agtattatgc
     7081 tgtgccccga cgttgcctac gacagcgccg cgccggccac gggcgagatc tacaagggct
     7141 ttttgcagct ggaaaagctg taccatgccg ggacgggggc gcatctgcgc tttgttccgg
     7201 tgtactgcgg taaaacaaag cgcatcgtca cgggcgaacc ggtctgcttt tcggacggcg
     7261 cgccgttccg cacccagagg gaggaagtgg ccggacgcat tgtggacggc ctgaacgcgc
     7321 tggccgccgc ctgcggcgag ctggagcggg aggcagcgga aaagagctag ggcaacaccg
     7381 cacaattcac ggctgatgcc ttaagcgccg cctcgcttgc ggattttccg gcaactgtca
     7441 caaaaatgct ggtgaataca caaagtattc gc
//
LOCUS       DAWBSB010000104         7460 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_1983_length_7460_cov_3.525186, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000104 DAWBSB010000000
VERSION     DAWBSB010000104.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 7460)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7460)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7460
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1983_length_7460_cov_3.525186"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    557..566
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1514 a         2059 c         2467 g         1410 t
ORIGIN      
        1 gttgttcagc atccgggcgg accttgtgtt caatatcgcg accgtcgcgg cgggcgtgat
       61 gatgctgatg ctggccacaa atctaaaaga ggacccccgc ggccgcaatt tctgcctggc
      121 ggccgcgctt ctgacggtgc tgggcatggt gccgggtatt gtgggcatcg tgtgtgcggc
      181 cgcgtcgtgg cctgtgtttg catggccgta tttcaaggct tccgttccgg aaaacggcct
      241 gcataaggca gcgtttctgg tgatggtgtg cgggctggtg ctgcttgtgg gcagttttct
      301 gcccgtgccg cagatgctgg ccgcgtgcat catcattgcg gtggcggcgg tgcagggcct
      361 tctggctttt ttgctgtatc aggaggcgtg accgcgccgt gcgggcccgg gcagacatat
      421 tccggcgggc cccatgcata cgatgatgca tgtcctgcct tttgggagcg ggcacagcgt
      481 tatggggaaa gggaaccgag agcatgaaaa aactgttgag aaccgctgcg gcagccgcct
      541 gcctgtgtct gctggcnnnn nnnnnngccg catgcgcgcc cgcgcggcag gcgggcggcg
      601 gggagcccgg cgcgccccgg gaggatttta cctctgtgcc gtccggtatg caggcggggg
      661 gcgagccgga ggccatggcc gcgcccgtgg cgtaccgcgc catgtgggtg agctatctgg
      721 aatggccgat gttcgatacc tccggcgcgc agggctttgc cgcgtcggtg ggctcgctgc
      781 tggacaactg tgcggctctg gggctgaaca ccgtcatcgt gcaggtgcgc cctttcgggg
      841 acgcggtgta ccccagcgca gtgtatccct ggagccacct tgtaacgggc gtgcaggggc
      901 aggaccccgg ctatgacccg ctggccattt ttgtggagca ggcccacgcc cgcggccttg
      961 ccatcgaggc atgggtgaac ccgtaccgcg tgcggctcaa cgacgccatg ccccccggcg
     1021 agctggcggc ggacaacccg gcgcttctgc acgagggctg ggcgctggag gcgaacggcg
     1081 ggctgtacta cgaccccgct ctgcccgagg tgcaggacat ggtggtgcag ggcgtaacgg
     1141 agatcgtgca gaattatgac gtggacggaa ttcagttcga cgactacttt tatcccacca
     1201 cagacgaagc gttcgacacg gagagttacg cgcggtacgg cgggggacag gacctggccg
     1261 aatggcggcg cgccaacgtc aacacgctgg tgcagaaggt atacgccgcc gtcaaggcgg
     1321 taaagccgga ggcggttttc ggcatcagcc cgcagggcaa caacgacaac aattacagcc
     1381 agcagtattc ggacgtggcg ctgtggcttt ccacgcccgg ttatgtggac tacatcatgc
     1441 cgcaggtcta ctggggctac aattatacgc tgcagaatgg ttcggcccgg ttcgcgttcg
     1501 agaatatcgt ggacgaatgg ctggccatgc cccgggacga ttcggtttcg ctggcattcg
     1561 ggctgggcgc ataccgtatc ggcgcggggg acggctccgc cacagagagc ggggaatggg
     1621 ccagcggcca caatctggcc gatatggcgc gcacgctttc cgtgcgcggc gcagacggct
     1681 acgcgctgta ccgttatgcc agcctgtaca gcggcggtga atatgcggca ctgatgcagg
     1741 cggaatgtgc ggctctggcc caggccaacg gcatcgggca gacggaaccg taacgcggag
     1801 aatacgaaaa aagcgccctc tttacagagg gcgcggcaga tggttgaaga aggcaaagag
     1861 gtaaaagaaa tgtccgggga aaaagcttga aaaaggggtg gagaacccct ttttcattta
     1921 aaataacccc gcgccgtgcg cggcgaacgg ggccacaggc cctgttcgta ggcagctttt
     1981 gcgccaagct aaagcgccct ctttacagag gacgcggcag gacggatgaa acgcggcatt
     2041 ccgccagagt gtcagaacag cccctttttc cattcccggc aaggaatgcc tgcggctgtg
     2101 agcgcggcgc acaaccgcgc cccgttttcc tttgtggagg gtgcgtccac gccgtagtgg
     2161 tacggcacca caagcccggg cagcacgtcc atggccgcca cttgtgcggg cactgatttt
     2221 tcatagtccg gcatgttcca gatgccgtcg cacaccacaa acagcacgtc gcattggaca
     2281 ttgtccacca ggacgtcggt gtcgccggag aggtaatagg tgaaccctgc gaactccaac
     2341 acgaggccga agccgaagct gacggggtgg tttttgtttt ctgcggccac cggttccagc
     2401 accgcgccgc attcaaagcc gatgcggggc gcttcaaagc tcagttcgct gatataatcg
     2461 tcgtcgatgc cgaaatcacg ctccagcagg cgcgccacct ccgccgtggt ggcaaagcag
     2521 gtgtcctctt tgcgcacccg ctcgatgtcc tccggcgaat agtggtcgcc gtggctgtgg
     2581 gtgatcacga tcaggtccgc gtcgtgggcg tcgtcaggca ccttatatgg gtctacatac
     2641 actacggcgt cgtcggattc aaagcaaacg catccgtgct ttacaaaccg gacaggttca
     2701 agggggtcca tagtacaact ccttattgtt tcgatgttta ttttttgtgg cttcaccgca
     2761 tactaactgt attccagacg cagaggcggc atgaaggaaa gaatacagct ttctttattg
     2821 taacgcctcc cgcgccaaaa gtcaaaaccg gagggatgca gatgaagccc cagaagataa
     2881 aatatgcgcc attgttcgct gctatgctgc tgtgcgtggc tttggcaggc tgcgacggag
     2941 cgcttcatga gagcggcgaa ggccgttttt cgcaaagtga aacccaagat ggaacccggg
     3001 cggctccgcg cgccgatgcc gtgcgcgcgg ccgcgggcgg cgatatcgca ctgcccgcca
     3061 cgctggcggc gccgcagacg gacagcacga tcaatacgct gctggacgcg ggcgtgctct
     3121 ccggcgcgtt cacgacggta aattcctgcc gcacgcggga tctttgcacg gcaggcgaaa
     3181 tcacagtccg cgtaagggca gagttggacg cccagacctc ccgcaaggat gcgaaattcg
     3241 cgttgtggaa actggcggac ggcagagcgg aatatctgga aaccgtgtat ttcacctgcg
     3301 acggtacggt gcagagctat acgttcacag ggctggccgc gggtgcgcgg taccgtgtgg
     3361 tgttcagcta tacggagtcc ggcgcgcggc gcatgtcggg cgcgttcaac gtggagggcg
     3421 tgacggccga gatcgaagag gaaacacagg aagagggggc ggtggccgcc ggcgcacagc
     3481 cgggaagctg accgcgtaaa agggggagga tgtcattgat cgaggatttc aagcatatga
     3541 aatggctgcc gctggcgggc ggcgcgctgc tgtgcgtgct ggggctgacg gcgctggtct
     3601 ggccgggccg ggtggcgaag atactgcctc tttgcatcgg cgctgcaatc ctggggctgg
     3661 gcctgtgcga ggtggcttca ggttttctgg caaagagctt tttgccagag tacatacccg
     3721 gcatgcgcaa actgcagggc tttgtaaatg tcgcggtggg gctggtattc ctgctcaacc
     3781 gcacgctttc gctgatgttc atcgccgtgg tgctgggcgt gtgggcagtg gccttcggcg
     3841 cgctgcgcct gagggacgcg ctgcggcggc gtgcgtccgg ccagccatgg ggttgctgtg
     3901 cgggtgacgc tgccgtaaaa ttcgcggtgg ggatatttat gctggccagc ccgctgggca
     3961 gcatggcggc ctggacggtg atggtggggc tgtttttcct gttcgtgggc gcatcggtca
     4021 ttttttctgc ggtatatctc gaccggctgc cccatgattt tgaggatttc tgaccccgcg
     4081 cgggcagatt ataaacaaag tgtaaaaacc gggccgcatg cccggttttt cttgcatttt
     4141 aagccgtttg aatgtaatat aaatagtaac attcatgcgc ataaagcgct gtggcaggga
     4201 ggccgctcga tggcaggaaa aatggtggaa aagaaaatac gctccgcgct gccgctgtac
     4261 attgcggcgg gcgcatttct ggtgtgcgcg gctgtgctgc ccgtatacag cctgccgggc
     4321 tttttgggcg cgtgtgccgt tgcggcgggc gcatattttc tgtcggacaa aaaaatcccg
     4381 ccccgtgtgg tgatggtgcc cgcgccggag gaccccttcg caacgggcga ggaggcgctg
     4441 gacgccgcgc tggaaaaggc gcgggacgat ctggagaagc tggaggtgct gaacgcccgc
     4501 attccggccg cggcgctctc ggcgcagatc agccgcatgg agaaagcggg cgcggccatt
     4561 ttgcagcagg tgcgcgaaca tccggaaaaa ggccgggata tccgcaagtt cgtcacctat
     4621 tatctgccca cggccgttaa gatattgacc acctatgccg acctttcggc cagcggcgcg
     4681 ggcggcgaaa acgctaaaag cctgatgtcc gacgtggaga agaacgccgg aaccattgcc
     4741 gccgcttttg aagcgcagct ggacgcgctg ttcgcgggcg aggtactgga tgtttcctcc
     4801 gacatcgccg tgctggacgg aatgctctcc ggcgacggcc tttccggcaa aaataatttg
     4861 aaattgtcat gacccgggcg gtcccgatac ctcattcaga aaggaacaaa acgatcatgg
     4921 aagaaaaaca aaccgcaccc gcgcttaccc tggagccaga cctctctgtt cccgccgttc
     4981 ccacactgac gctggacccg gagcccgccc cccctgcgca ggaggaaaag aaacctgcag
     5041 tggagccgga gcccatggac gcgggccttt cgcccgaaga gaaaaaggca gtggacgaat
     5101 tcgcggaaaa aatcgacctg cgcaattcca cggtggtgct gcagtacggc gcggccagcc
     5161 agaaaaaagt ggccgatttt tccagcagca cgttggaaaa tatccgcacc aaggacctgg
     5221 gcgaggtcgg cgagatggtc acgggcctgg tgacggagct taagagcttc gacgccaccg
     5281 aggaagcgcc caagggcatc atgggctttt tccgcaaggc caccaattcg ctggatacct
     5341 ttaaggcgaa gtatgccaag gtggagacca acgtggataa gatcgaggct gtgctggagg
     5401 gccatcaggt acagcttctg aaggatatcg ccatgctgga caaaatgtac gagatgaacc
     5461 ttgtgtattt caaggagctg acgatgtaca ttctggccgg taagaaaaag ctggcggagg
     5521 tgcgcgcaaa cgaattgccc gccctgatcg aaaaggcgaa aaaatcaggc ctgcccgagg
     5581 acgctcaggc agccaaggac ctggacgata tgtgcacgcg ctttgaaaag aagctgtacg
     5641 atctggagct gacgcgcaac atctcggtgc agatgtcccc gcagatccgc ctgatccagt
     5701 ccaacgacac gatgatggcg gaaaaaatcc agacctctat cgtcaacacc attcccctgt
     5761 ggaagagcca gatggtgctt gcgctgggtc tggcccattc ccagcaggcc attgaggcgc
     5821 agcgtgccgt tacagacatg accaacgagc tgctgaagaa aaacgcggcc acgctcaagc
     5881 agggtaccat cgctgcggca aaggaatctg agcgcggcat cgtggacatc gagacgctgc
     5941 agcagacgaa ccgcagcctg atcgaaacgc tggacgaggt cgtgaagatc cagaacgaag
     6001 gcagagagaa gcgcgctgcg gccgaaacgg agctgggccg catcgagggc gagctgaaag
     6061 cgaagctgct ggagatcgcc cgctgagttt gtacttgtga cgggaataag agaaaatgcg
     6121 ggaggatagc gtgaaaaact ggaaagaaaa ccgtgcgctg gcctgcgtgg tgctggcggc
     6181 tgccattttg ggcggtactt tcggcatcgg cggggccaag ctgagcagcg cggcccgcag
     6241 cgtgaatgag gcatacgcgc agaatatcgc gggggacctg gcgctgcgcg ccgccgccgc
     6301 gcagaatatc atcgccgagg gtgaagcggc gctgggcgcg gatgccgcgt ccgtgaagac
     6361 ggcaaaacgg gccgtggagg cgctggaggc cgcggagggg ccggcacagg aatacgccgc
     6421 aaacgcggcc ctcaccgcgt ctgtcggcat gctgtatgag gagacgcgaa aggtctccgg
     6481 cgacgataag ggcagcgtgc tgcaaacaca gtggtcggaa tttttgagcc gtggcaatat
     6541 catcgacaat tcggcggacg catacaatga gcaggcccgc gctgcgcaaa aaaagctgtc
     6601 cggcttcccg gcgtcgctca tcgccggcct gacaggcgca caggcggaag aattttccgc
     6661 ctgagtgcgg ccctacattg aaaaggagca tccatgaaga aaatccaacg atttctgcgc
     6721 ggcacggcgc tgctggccgt gctgtcgctg ctggtttcgg ccacggcatt tgccgcgtcg
     6781 gttccggacc caacatctga tttttacgtc aacgattacg ttggtgtgct ttcaggtgat
     6841 actaagagtg atattgtgag caaaaatgac ggtttgtata atgctacggg cgcgcagatc
     6901 gtcgtgacgg tggtgcagga cacaggcggt gtttcgatgg agcagtacgc ctatgacatg
     6961 gccaacgcct ggggcattgg cagtgctgaa aaaaacaacg gcgttttgct gctgctttcc
     7021 gtcggggacg acgattacca gtgcatacag ggcagcgggc tggaaacgct gctgccaacc
     7081 agtacgctca gccgcatttt acaggaggac ctggagccgg atttcgcgga taaaaactat
     7141 gacgccggtg tgaaaaaaac atttgcgtcc ctgtatgacg ctgtgagcag catctacggc
     7201 tacgacggca gcacaggcgg cgcggcgaat ccgtctcccg tgccgcagcc ggactacgac
     7261 tatggatacg gatatggcta tgacgacggt taccgggacg acggcggctt cagcattttt
     7321 gggatgatcg tgttcctcgt gatcgtgctg gcgctcatcg gcgcgttcag cgggccgcgc
     7381 cgccggcgcg gcggccgtta cagcacttat tacagcggcc cttcgttcgg cacgggcttt
     7441 ttcctgggca gtatgatggg
//
LOCUS       DAWBSB010000105         7275 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_2028_length_7275_cov_2.958864, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000105 DAWBSB010000000
VERSION     DAWBSB010000105.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 7275)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7275)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7275
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2028_length_7275_cov_2.958864"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    627..636
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1537..1546
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4346..4355
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6110..6119
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6925..6934
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1399 a         2371 c         1976 g         1479 t
ORIGIN      
        1 cctttctcaa ataggcttcg cggaaccgcg cgcgccccct tgtaaggcgc gtgcgcactg
       61 tgttttcgct cacgtccagc gtccgagcaa tctgtttgac aggcatgtca ttgaggtaga
      121 acagcgtcag caccaacctg tcgttctgcg aaagcttctg cagcgtacgt tttacatcca
      181 ttgcttcctc cccctgctct tccgcagcct gctcgggcag aaactccaca ggcgtctccc
      241 gtttttttcg gcgcaggata tcatagcaat tgtaaatgag cacccgcgtg agccacgttt
      301 tgcaatagcg cggcgcctcc agtttcggca agctgcgcca tgcctccaaa acggtttcct
      361 gtatcgcgtc cgccgcgtcc tcgtcgctgt gcaaaatcgc gcgggccgcg cgataaagcg
      421 cggctttata ctgttccatc agcgccacaa aagcgtcctt gtccccacgc tgcgccctcc
      481 gcaccagttg atcggtcaac ctttacacct tcctttttct ttgtgatcac gcctattaga
      541 gctgcgaacc tcccaaaagg ttacacctgc ggcaaaaaaa ctcaaagctc ctttgccgcg
      601 cgcaaaaaag ctcccggcgg cgggggnnnn nnnnnngcac aggcttcttc gcctgcgcct
      661 tttcccgccg ccgggagcca ttgtctgccg ttctgtgctt ttacagcgcc atacgcatag
      721 gaacgtgggg atatccttcg tccagatacg gttcaccgta cggcgcaaag ccaacggcct
      781 cgtaaaagcc ctgcttgtcg tactgtgcgc ccagctccag cgtgcgcgcc ccctgcgccc
      841 gcgccttatc cgccacggcc gccagcagca gccggcccac gccctgcccg cgcaggcccc
      901 gctgtacggc aatgcgcccc gcatgccaca cgcccggcgc gtccgcaaac acacgcgccg
      961 tacagcgcgc ggcccctgtt tcgtccaggc ccaatacatg cagggcgttc ttgtcctgct
     1021 cgtccctgtc cgcctccagc gaaaagccct gctcgtccac gaatacctct ttgcgcagcc
     1081 catgggccag cgccatgcgc gctgcgtcgt caaacccaaa ccattcaaag tgcagcgtca
     1141 attttattcc tccctccgcg gcgcggcatg cgccgccttt ggggaatatt gtagccgacg
     1201 gacgttaaaa aagcaacagc acacgcagca gcagcatggt gaccacgccc ggcgcgccca
     1261 gcaccacggc tacaaacgcc gtggcgtaat taagcgcgat ggaaacgccc gtgtaccctg
     1321 ccagcaaatt caccgcgccc agagatgcgg ccccgcacac cgcgctgccc agcgcccgca
     1381 gcagcggccg ctttgccttg gatgctgcca gcactacgcc gatcacgctc atcagcgccg
     1441 cgaacactcc gatggtctgt accaggatca ttttcctcac ctcgccacag catatgcgga
     1501 caaggccccc ggtatgacac ccggatgcgt ccggggnnnn nnnnnncggg ccggaagccc
     1561 cggctccttc cgctactgtt cctgtgccgg aacgaaatcc acattgccct gagagacatc
     1621 cacgcccgcc acacgcacgc gcatgacgcc gcccagccgc cattcccggc cggacagaat
     1681 atcccgcagc gccacgccct cggtgagcgt aagcgcgttt tccgaaagcg cctgcgcgcg
     1741 cacaaggcct tctacggtat tgggcagctc cacatacagg ccgaactgcg tgatcgacga
     1801 gatcacgccg tcgaattcct ctccctcgaa gcggcgcatg tattcggcct tgtagcagtc
     1861 ctccacatcg cgctccaccg tcatggccag cacctcgcgc tcggaggact gcaccgatgc
     1921 ttcggccgcg aactgcgcat attttttctg caccgcgccg tcgtccatac ccgcgcaaac
     1981 gtcggaaagg atacggtgga tggcaaggtc cggatagcgc cggatgggcg aggtgaagtg
     2041 ggcgtaatct gccagcgcaa ggccgaagtg gcccttgggc tgcggctcgt actttgcctt
     2101 ggccatgctg cgcagcacac ttgtgtgcac cgggcgctcc agattggtgc cccgggtatc
     2161 ggccaacagc ttcgcaagct ccagcgtggt gggcgtatcg cccgcaaaat ggaagtccac
     2221 acccgccgcc gtcagcgtct gcttcagctt ttcgatccgc tctgggtccg gcgcttcgtg
     2281 cacacggtac acgaacggca gcttcagccg ccgcgccagc gccgccgccg aggaattggc
     2341 cagcagcata aattcctcga tcatctgctc cgcctcgccg cgttcgcgct tcaccacgtc
     2401 cacacagcgg cccgcctcgt ccagcaccag ctttgcctcg ccgctctcaa tatccatcgc
     2461 gccccgggct gcgcgcagct tggccagctt gtggtacagc tcgcgcatca gcggcagctc
     2521 ctgcgccacg gcggcgtaac gctggcgcag cgcgttgtcc gccgtgccgt caaaaatggc
     2581 gttcacctct ttgtacacac ctttcacgcg ggaacggatg acggatttca cgaacgcgta
     2641 atccactaca cggcccgcgg cgtccagctc catcatacag gagaatgcca gccggtccgc
     2701 gccctcgttc aggctgcaga tgccgttgga gagctggcgc ggcagcatgg gaatgacgct
     2761 gtccgcataa tataccgatg tgccccgcgc aaacgcctcg gcgtccagcg cgctgccggg
     2821 gcgcacataa tggctcacgt ccgcgatgtg tacgcccagc cggtatcccg tatctgtctt
     2881 ttccagggaa atggcgtcgt cgatgtcttt cgtctcggcg gaatctatgg tgaagacagg
     2941 catgccgcgg taatccctgc ggcccgccgc ctctttttcc cccacggccg cgccctcgta
     3001 ttttttcgct tcagcctttg ccttgagcgg gaaatggcgc gagagccccg cgccatacag
     3061 cagtgccttt gcgcactgct tggcctcctc ggcgctgccg aagcgcatgg ctacgcccgc
     3121 ccggtggtcc tcgtgggaat cgccgcgctc cagaatctcc accgcagcct tttcaccgtc
     3181 gcgcgcgccg ccgtcggcgc tctttttgat atagatgggg cagtgcggac acgcgtccgg
     3241 caccagcgcc agcctgccct caatggtctc caccgttccg gtaaagcggt tgttttcctt
     3301 caccacggcc agcacctcgc cctcggacga gccctctacg cgcggatgct cgaacagcga
     3361 caccagcagc gtgtcgccgg gcatggctcc catcaggtat ttgcccggaa tgaagatatc
     3421 ctgcccgtcg ggctgcacag gccgcgcgaa gccaaacttg cccgccagct tcaccagcgt
     3481 acactccacg gcgttccctg ttttcgggtc cggcacggcg tacacgccct tgcggcacac
     3541 caccttgttc cgctccacca gctcgtccac ggcgcgggcc acttttttgt cgttgcccag
     3601 cttcgccttc agctctttca ggcggcgcgg cttcttttgc agctctttca gtatttttgc
     3661 tttgattccc ataatatgct ccttactcca cacacataac aatacttttc cccggtcttc
     3721 ggagatatat agcaaaaaag cccgccaaaa gctggcgggc ttttttctgg cttatttcag
     3781 atagatgctg atgacgccca ccaggatagc gagcacaaag aacacgatgg ccgcatactt
     3841 ggtgtatttg gcaagcatgg cattggagga acgcgagcgc ccttcgttgc ccatcatctc
     3901 accgccggcg atcgcaccgg acaggccgtt gcccttggat tcctggaaca gaaccatcac
     3961 gatgatggca atgctgcaca aaatcagcaa aacaccgctg ataatctcta aaacacccat
     4021 tttgaaacct ccagttcatt cgcgtaaata actataccat agcccgcggc aaaaagcaag
     4081 catatttctc gcgcggcgcg cgggtttgca aggcctttcg tgttttatcc cgtcctttgg
     4141 cgcgcccggc gcccggccgc ggggcaaatt tgcaaaaaag agcggctttt ccccgcgttt
     4201 tgtggtatgc tattaacagc cgccggtgcg cctgtatggg cgcaccggcc atgcgcgcgg
     4261 cgcacctccg gggcgtcccg ccgctttcta aaaaacgagc aaggagagat catatgattc
     4321 accgccgtaa agccggccgc gcggcnnnnn nnnnncgcct gcctgctgtt ttccgcctgc
     4381 gggccgaacc tgttccagac gcaggccgag gaacgcccgc ccgttgtgca tgccgatgtg
     4441 gatttcagta cgctggagta cacacgtccc gacctggacg cgttgaacgc gaagatcgac
     4501 acagcgctgg agcaggtctc cgccggagac aaggaagaag aaacattggc cctctacgac
     4561 gacattctgg cggatatcag cgagctggac accatgagtg ccatcgccgg catccgctac
     4621 aatatcgacc ttacggacga atattacgaa gccgaggagc tggctctgga cgaagcatac
     4681 acccgtctgg acaaccgcat gaacgaactg acgggcgcga tcctggaatc cggctacggc
     4741 aaggccgccc atgcgcgctg gggcgacggc ttcgtggagc gttacgaggt gaacagcaag
     4801 ctcaattctc ctgagataga agagctgagc atacgggaac aggcccttgt gagcgagtat
     4861 cagaagctgt ccgccaccga atatacggcc gagcgcgacg gagaagccgt gacgctgaac
     4921 gatctggatc tgacgcagga atccgatatc gcgctgtact atgcgatcta tgaaaagcgc
     4981 aacgcagagc tgggacagat ctaccgtgaa ctggtgcagc tgcgcgtgga gatcgccaaa
     5041 aagctgggtt atgacagcta caccgattac gcctatgacc tcctgggccg cgatttcacc
     5101 aaagaggatg cggccgcgtt cagcgaaaag gtcaaggagt acctggcccc catcagcgac
     5161 gcgatctaca accgctatta cagcgatatt gccgcggcac aggcccgcac ggacgttacc
     5221 tttgacgacg gcatccctgt gctggaacag gcgctgaaga acggtggata tcccgccgcc
     5281 atgagcgacg cgctgaccta tatgctggaa cacaacctgt acagcttcgg cggcggcgca
     5341 aacaaaatgg ctgcgggcta caccacgctg ctgaaccgtt acgccgcgcc gtatatgttt
     5401 gtgaataccg attattatac cgacccgggt acactgttcc acgagttcgg ccactattac
     5461 aacttctacc tcatgggaga gacactctgg aacgacggca acaatctgga tctggcggag
     5521 atccactccc agggtctgga agtgctgatg ttcgacacct acgaggatat gtacggcgag
     5581 gacgcctcct atatcgagta cgcacaactg atgaacctgg tggattccgt acttcagggc
     5641 tgtgccgagg acgaattcca gcaggccgtg tttgaggacc cggacatgcc gctggacgag
     5701 atgaaccttc tgcatgccca gatctatcag gactacatgg gttatcctct ggtttacgag
     5761 tgggtggata tccaccatca tttcgaaacg ccgttctact atgtcagcta tgccacctcc
     5821 gccgtgtccg cgctggaact ctgggccgac gcgctggaaa accgggacaa agccatgcag
     5881 atctatgata agctgacgca atataccatc aacgtggaat acctggaaac acttaaggaa
     5941 gtggggctga gcgacccgtt ctcctccgat tgcgtgcagc gcgtggcgca ggcgctgaat
     6001 gacgagatgc agctttccgg caagccgggc tccaaggccg catgaccttt attccgccac
     6061 cattccttcg aatcaaaaaa gccgccgcgg ctcccgctga aacggggagn nnnnnnnnnc
     6121 tccccgtttc agcgggagcc gcggcggctt ttttgattcc gttttccggg cccttacagc
     6181 agctgcatct gctcggcggt gtcctcgccg cgcaggatag cacctgccgc gggcagcgta
     6241 cgcacgcggt agcggtgcgg gtccgtcaat tccagattgg ctgcgcggcg cacatggatg
     6301 atggactggt tctttttcag cgtaacaacg ttcacacctg cggtattctt tgtctgcttc
     6361 gcgggcacct gggccgaatt caccagcagc atgcggcctg cgctggtgta aatggcaagc
     6421 tcgcattcct cgtgcaggtg tgccgcgtac accagcggcg atttgtcgct gaaggcattg
     6481 agcagcttgc gccggttctg cttggtggcg tagctttcca tgggcacctt tgccgctttg
     6541 ccgttggcaa acaggaagaa catatggcct ttgtaatccg ttgtaagggc catatacacg
     6601 ggcgtttcgc cctcctccat gcccagccgc gcggcgatat agtcgcccat cacgctggcc
     6661 ttgccgtcgt caaaatcgcc cacgcggcac ttgtacacct ggcatttgct ggtgaagaac
     6721 aacagctcgg cgttgttctt cgtttccatc tggacggtga tctcgtcgcc ctccttgagc
     6781 ttttgctcac cggacatccg cagggactgc ggcgtgatct tcttaaaata tccttcgcgg
     6841 gtatagaaca cggtaacggg ataatccggc atgccttcgt cctcgtcctc atcctcgggc
     6901 agctccgcct cgtacaggat ctcgnnnnnn nnnncgatga tgaccctttt gatttttgcc
     6961 gggctgttca ggatatcctt cagccgggcg atgtccgctt ccaggtccgt cgtttcctgt
     7021 gtacgcctga ggatgtattc gcggttcagg tggcgcagct tgatctcagc cacatactct
     7081 gcctgcacct tgtcgatgcc gaagccgatc atcaggttgg gcaccacctc ggcttcctct
     7141 tcggtctcgc gcacaatgcg cacagccttg tcgatatcca gcagaatggc ctccaggccc
     7201 ttgagcagat gcaggcgctt ttccttgccc tgcagatcgt aatacgtacg gcggcgcacg
     7261 cactcggtgc ggaac
//
LOCUS       DAWBSB010000106         7258 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_2032_length_7258_cov_2.873247, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000106 DAWBSB010000000
VERSION     DAWBSB010000106.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 7258)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7258)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7258
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2032_length_7258_cov_2.873247"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1351 a         2146 c         2321 g         1440 t
ORIGIN      
        1 ctatggaggg cttcatgata agcacgcgca tgccgcgctc ttcgtagttg taggctacct
       61 gcatcagcgc ggtgctcttg ccgctgttca tggcgccgta tcggaaatat agctttgcca
      121 tttggtaaga ccgtccctat cctgtcgttt taatcgttgc gcacacggta gcccaggctc
      181 atcaggctgt cgcccaggtg gacgttctcc accgtgatac cctcgtagtc caccgagagg
      241 tcgtagtggc tgaagttcca gtagcccttt acaccggcct ccaccagcgt gggggctacg
      301 gccttcgcgc ccgcgcgcgg gatgcaaagc accgctacgt ccgggtggtg tgcggcgcag
      361 aaagcggcca gctcggccac gtcctgcacc atcacgccgc actgcagctc ccggccaatc
      421 tcgtccgggc ttttgtcgaa tgcggccatc aggtgatagc cccgggcctc ggcggaaaga
      481 tagccggata tggcgcggcc cagacggcct gtaccgatga gcactgcgtt gagattcttt
      541 tcactgaaca gcagcttgga tatctcgctg agcagcacat ccacatggta gccgatgccc
      601 tgctggccga aaccgccgaa gcagttgaaa tcctgccgga cctgtgaggc tgtggtgccc
      661 atcattcggg caagctcact ggaagacacc ttcacgatgc cgttgttgtg catgtccgaa
      721 atatagcggt agtatttcgg cagccgcttg atgacgggaa gggagacttt attctggttt
      781 tgcgccgcca ttgtcttcac caacttcttt tcgtctaaat taaagggtct cgatggtgcg
      841 catcacgtaa tcgccgaact cggtgcaggt gcagccggtg tcgcggcccg tgacggtgag
      901 cgccttttcc gtgaacatgc atttgtccag cgcggcggtg agcttgtccg cctgggcctg
      961 gtatccgatg tgggacagta gcatcacgct ggcgcgcagc atgctgcacg ggtctgcgta
     1021 ctgggcgcgt ccctccttca ccatgcgcgg cgccgagccg tgaatggcct caaacatggc
     1081 gtagcgcttg ccgatgttgc cgctgcccgc cgtgcctacg ccgccctgga attcggccgc
     1141 ttcgtcagtg atgatgtcgc cgtacaggtt gggcagcacg aacacctgaa aatcggtgcg
     1201 gcgctttttg tccaccagct tggccgtcat gatgtcgatg taccagtcgt ccgtggtgat
     1261 gtcggggtat ttttcgccta cggcctggca cagcttcaaa aacttgccgt ccgtggcctt
     1321 gatgatattg gccttggtga cgatggacac gcggttcttg tggttttttt gggcgaaatc
     1381 atacgcaagc tctgcaatgc gctgcgtgcc gtcagtggta gtgatggtga aatccacaga
     1441 gatgccgtcc tcggtctcga agccgcagct gcccagggtg tacgcgccct cggtgttctc
     1501 acggaagaac gtccagtcga tgccttcctc gggcaccttc acgggccgta cgttggcgaa
     1561 cagatccagc tccttgcgca tggcaacgtt ggcgctttcg atgttgggcc attcgtcgcc
     1621 tgcgcggggg gtggtggtag gccctttcag gatgacgtgg catgccttca gttcggccag
     1681 cacgtcgtcg gggatggcct tcatgtgggc ggcgcggttc tcgatggtca ggccgtcgat
     1741 ctccttgaac gccaccttgc ccctttccac ctcgtctttc aaaagaaagc gcagcacgcg
     1801 ctcggcctca tgggtaatgg tcgggccgat gccgtcgccg ccgcatacgc caatgacgat
     1861 ctgcggcagt gccgcgtaat ccacaaaatc tccctgtgcc cgcatggcgg ccacccgttt
     1921 tttctgcgcc tcgatcagcg cgccgaattt ttcttttgca gcagtgatgt tttccaattt
     1981 gcgtgcctcc gttgtcgttt attttttgcc gccgcgcatg ccgcgaaagg cggtgcggcg
     2041 cggaaatgcc ggtgtttacc ggtttttttc gcgggtgtag ttcaaaaggc ctcccgcgcg
     2101 gatgatctgc gcctggcgcg tgctgatgtc gcagcgcagg gggatgacgc agcccgtggt
     2161 cttgttcgtc agcgttaccg cgccgttttc gatgcccgcg atcacgccgg aaagctccag
     2221 cgtgtctccc agggagatct tttcataatc tgcggcattc tcgaattcca gcggcaggat
     2281 gcccgcgttc accaggttcg ccttatggat gcgcgcaaag ctgacggtga cgaccgcgcg
     2341 gatgcccaga tacagcggca ccagcgccgc gtgttcacgg ctggagccct ggccatagtt
     2401 ggcgcctccc acgatgaagc cgccctctgc cgccatggcg cgcgcgggga attcggggtc
     2461 gcacacttca aagcagtagt ttgccagatg cgggatattg ctgcggtaag gcaggatctt
     2521 gctgccggcg ggcatgatat ggtccgtggt gatattgtcg cccaccttca gcaggcaggg
     2581 cagctccatt gtgtctgtca gaggctgcgc cacgggcagg ggcttgatat tcggcccgcg
     2641 cagcacctcg acgctttcat attcgtcctc agaggcgggc agatccacca tgttgtcgtt
     2701 gatgggaaag gcctccggca tggcgacggg cgcggccgca ggcacggagg tggggtcggt
     2761 gatatacccc gtcaggcagc tcacagccgc cgtttcaggg cttacaaggt atacgccggc
     2821 gtcccgcgtt ccgctgcggc cctcgaagtt gcggttgaac gtgcgcaggg aaacgcccgc
     2881 ggagttgggg ctttggccca tgccgatgca ggggccgcag gcgctttcca gaatgcgcgc
     2941 gcccgcgtcc agcagggcag tgagcgcgcc gttcgcgctc agcattgcca gcacctggcg
     3001 gctgcccggc gcaatggcaa ggctcacttc gggagatacc gttttgccct tcaggatctc
     3061 ggccacggtc atcagatccg catagctgga gttggtgcag ctgccgatgc acacctggtc
     3121 taccttcgtc atggaaaggc tgcgcacggg cttcacattg tccgggctgt gcgggcacgc
     3181 ggccagaggc tccagcgcgg aaaggtcgat ctcaatgacc tcgtcgtaca cggcgtccgc
     3241 gtcggcggcc agcggcatat aatcctcttc gcgcccctgc gccttcagaa aggcatgggt
     3301 gacttcgtcg cttggaaaaa tggaggttgt agcgcccaac tccgcgccca tattggtgat
     3361 ggtggcgcgc tggggtaccg tcaggttttt cacgccctcg ccgccgtatt ccacgatctt
     3421 gcccacaccg ccctttacgc tcatcacacg cagcacctca aggatgacgt ccttggcgct
     3481 caccatgggg cgcagcgcgc cggaaagctt taccagcgtc gcttttggat aggggatata
     3541 atacgcgccg ccgcccatgg ccacggccac gtccagaccg cccgcaccca tggcaaggct
     3601 gccgatgccg cccgccgtag gcgtgtggct gtcggagccg atgagcgttt tgccgggttt
     3661 ggcgaagcgt tccagatgca cctgatggca gatgccgttg cccgggcggg agtagcgcac
     3721 gccgatcttt ttggcgatgg tgcggatgaa acggtggtcg tcggcgttca taaagccgct
     3781 ttgcagcgtg ttgtggtcga tataagcgac ggaaagctcc gtgcgcacac gggggatacc
     3841 catggcctca tattccagat acgccatggt gcccgtggcg tcctgtgtga gtgtctggtc
     3901 gatgcgcagg cccacctcgc agcccggctc cattttgccg tcaacgaggt gtgcggcgat
     3961 gatcttctgt gcgatggtct gtcccattcc aaaacccctc tccagttttc ctgcgctgcc
     4021 gcccgccgcg cgggcgcata tgcacagtat ggttatatac ttatttataa tacctgtttg
     4081 ataagttatt gtcaatgaaa cggctgtgaa aaacgggtgc gccgcaccgt gtgaggcaaa
     4141 aataaaatgc attactattt ttgcgcggaa aggcgataaa acacggcgcg gcagggaaac
     4201 ataagggaaa aagcgaaagg cgggagacca tgaacagcga tacgaaaaca aaagcgcagc
     4261 cgcaggacag cgaggaaaca gaggcggaaa ccgacaaagc acgctttgaa aaggattata
     4321 tactggagac aggctctgtg ctgcgcacaa agggaaggca tgccatccat tgcctgacgg
     4381 tcatcgggca gatcgaggga cattccatcg cgcctcagac gcaaaagacc accaagtacg
     4441 agcatgtcat tccacagctc gtttccattg aagaagaccc ggaagtggag gggctgctcg
     4501 tcatcctgaa cacggtgggc ggcgatgtgg aggcgggcct ggcgctggcg gagctcatca
     4561 gcggcatcac gaagcccagc gccacgctgg tgctgggcgg cgggcattcc atcggcattc
     4621 cgcttgctgt atcagcacgg cgcagcttca tcgtgcccac ggccaccatg acggtgcacc
     4681 cggtgcgtca ctcgggcctg gtgctgggtg tgccccagac catgcgctat ttcgagcaga
     4741 tgcagcagcg cattacgggc tttgtggcgg gccattccca tatcagcgag gcgcgtttca
     4801 ccgaattgat gatgcacacg ggtgagctgg tgatggacat gggcagcgtg gtggatggag
     4861 aaacagctgt ggcggaaggc ctcatcgatg agctgggcgg tttgggcgac gcgctgcatt
     4921 atttgtaccg cgccatcgaa gccccgcgcg acggcgcgcc ggagcagcgg aaagccggag
     4981 gaaagccgcg ccgtgcgccg cgcgcgcaca gtgctgggaa atgatgccaa gacccgggcg
     5041 ggcctgccgc aagggcgtac aaaagcatgc agccgcaacg gttccaactg tacaagcgga
     5101 gcgtttggtg gtacaatgta caagtatcag tgtgcaaaaa tgcgcaaaag ccatgcgtgc
     5161 ggcgcatgaa aaaatccatc cggcggggcg tttgggccgc gccgggtaca tacaggggta
     5221 tcggacaagc tatggcatcg acaaacaggg caagaaaaac aaatacaaag acgaatacgc
     5281 gcggctccgg ccagcgcggg ggcaggcggc gtgcgaagcc gaagcccgat atgaacgcca
     5341 ggtggagcat ccttctgttt ggcatcggcg tgctggtgtt cgcgctgatc gtggtcccgg
     5401 gcgcgtcggt gtggaactgg gtgcgcacga acatcctgtt cggcgtgttc ggcgtgggca
     5461 gttatgtgct ggcgcccatg cttttataca ttgccgtatt ggtggcctgt gagcgcccgg
     5521 tgaaaggcaa gctggcaaag gcgcttctgg tgctgatgct ggtgtgcggc acgttcctta
     5581 ttttttcaaa aacagacctc acgggcctgg gcttctggga gggcatggcg gtgctgcgca
     5641 gcaccggcgc gtcggaatgg gggctcggcg gcggcatgct gggcgcggtg ttcggctgga
     5701 cgctgctgct gctgtgcggc cgcccggggg caaatatcct cattgtaatc ttcatgctga
     5761 tgggtctgat gctgttcaca ggcgtcacgc ctgcggatat ctggtacttc ttctccggcc
     5821 atgcgggccg catgaaggat tccatggacc ggcgcgccga tacacgtgcc cagctgcgcg
     5881 aagcgcgcgc acaggaggcc gagcaggccc gccttgcggc tgaggaagaa gcgcggcggc
     5941 atccgctgcc cgccggggac gatgtcccgc ccttcgacat ggatgcagcg gcctacgaag
     6001 ccgcgcgcag cggacgcaaa cgccgtgcgg ccattgatat cgacattggc ggcgaggctg
     6061 tcagcagtac tgaactgaaa gaggacgaga agcctgtcat tggtcccggc gggaccttcg
     6121 gcatgtatcc cggcaaaccg cgtcagccac gcacggcggc gcccgcgggc gtccccgcgc
     6181 cgtccgctcc tgcggcggcc gcgcccgggg ggtacaccgc cgcggcgggc agccgcactg
     6241 taccgcagga ggaagaagcc tccgcgcccg tgcagccgca gcaggagccc cctgtgcagg
     6301 agagcgcgga agacgcgctg gcgagccttg tgaaaaaggc ggcgggcccc gcgccggagc
     6361 ttgtggtgga tgccaacgac cagcttacta tagcgcccgc cgcgggcgaa ggcgagacgg
     6421 gctacgctta tccgcctctg agccttttcc gcaagcagca gcccacagat gaaaagggcg
     6481 tggaagagga gctgacacac aacgcggatc tgctggtgaa aacactgtcc agcttcggcg
     6541 tacagacgcg tatgatggat atcagccgcg ggccgtctgt cacccggtac gagctgcagc
     6601 ctcttgcggg cgtaaagatc agccgcatca cgggtctggc ggacgacatc gcgctcaacc
     6661 ttgctacggc gggcgttcgc atcgaagcgc ccatcccggg caaggcggcc gtggggatcg
     6721 aggtgcccaa ccgcaactcc acatccgtca gcatccgcag cattctggaa tcgcccgtat
     6781 tcaccaaaag cgcttcgccg cttacgctgg ccttgggtaa agacattgcg ggcgcatgcc
     6841 aggtggcgga cctgaccaag atgccgcatc tgctcatcgc aggcagcacg ggcagcggca
     6901 aatccgtgtg catcaacgcc attgttatga gctttttgta caaatcctct cctgaggatc
     6961 tgaaactcat tatgatcgac cccaaggtgg tggagctggc cgagtataac ggcatcccgc
     7021 acctgctgat gcccgtcgtc actgaaccgc ggaaggctgc gggcgcgctt ggcgcggccg
     7081 tgggcgaaat ggaaaagcgt taccacctgt tcgcggagac caacgtgcgc gacatcaaga
     7141 gctataaccg catggccgca gagacggagg gcatggagaa actgccctac atcgccatcg
     7201 tcatcgacga gctggccgac ctgatgatgg tggccggcaa ggaagtggag gactatat
//
LOCUS       DAWBSB010000107         7197 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_2049_length_7197_cov_3.121955, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000107 DAWBSB010000000
VERSION     DAWBSB010000107.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 7197)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7197)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7197
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2049_length_7197_cov_3.121955"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1160..1169
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4433..4442
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5581..5590
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5978..5987
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6206..6215
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1338 a         2184 c         2205 g         1420 t
ORIGIN      
        1 ggaattatgg gacttcgagc tcaattcctg ttgcacttag tttgacaatt cactgataaa
       61 gaattttatt gagaacgcga attttgagga taccgggttc agttatacgc tgtctctcat
      121 atccggcaaa tataaaatgg tcattctgta ctgccttatg gagtatgaag tcgtgcggtt
      181 caatgaactg aaacggtatt tgaaaaacat atctgataaa actctcagta caaacctaaa
      241 agagttagaa tcagaccggc tgattataag aagagagtat ccgcaaattc caccaaaggt
      301 agaatacagc ctcagcgacc gaggaaaatc cctgatggcc gttctggacc agctgtgcat
      361 ctggggcgaa aagaacaaat tgggatagat aacagcttta tcgttctccc cgctggcatt
      421 ttccccgaaa gtgtcatagt gggttacaca ataaaaaagg acaaggcaaa ccattgcggt
      481 ctgtcttgcc cttaacttct ttatgaggcc ttctactccg gcggaggcct ttttgttgtt
      541 ttcatttgct ttggtcggag aattccgccg aaaggacggc gtacagccag gcgtccacaa
      601 agcacccgtc tttccggtag cgtcggcgca gtatgccctc ccggcggaat ccgcattttt
      661 ccagcacgcg cacagaggcc gcattgcccg ccagtacttc tgcacagatg cggttgaacc
      721 ccgcctgtgc gaagccaaag cgcagaacgg cccccagggc ttcaggcatc agcccggcgc
      781 cgcggtacgc gggtgaaaga gcataggagg tctcggcgca gcagtgcgcc tcatccacgc
      841 cgtgcagatt gacgacgccg gccagcgtgc cgctttcgcg cagcgtgacg gcccactgga
      901 gaaaatccgg ctccgcgtaa cgtcccagcc agttttccag ccgcacctgc gtttcctgca
      961 gagcagcgca gggctcccag ggcagatacc ggcagcaatc ctcccggccg gcccagccgt
     1021 gaaagacagc gggggcgtcg ccgggcaaaa aacgccgcag gacaaggcgt ccggtctcca
     1081 gcggcacggt gccttgaagc ttcatttcaa atttccccct ttgccggttg gaaggatggg
     1141 ccgcggcgcc ccgaaaaggn nnnnnnnnng gtttatgcct cgaaggcgtt ccgtgtggct
     1201 tctgcgcggt actgatgagt gtacaggctg taataatatc cgcccagcgc cagcagctcg
     1261 tcgtgggtgc cggcctcggt gatgcgcccg ccgcggatga caaggatctt gtccgcgctg
     1321 cggatggtgg acaggcggtg cgccacgatg aagctggtgc gccctgtcag caccgtctgg
     1381 atggcgttct ggatgagctg ttccgtctcg gtgtcgatgc tgctggtagc ctcgtccaga
     1441 acgaagatgc gcgggtcgga gaggaccacg cgggcgaagc acacaagctg cttttgccct
     1501 gtggaaaggc ggccgccgcc ctcgcccacc tctgtgccgt agccgtcggg cagggcttcg
     1561 atgaagcggt ccgcccgcac catggcggcg gcgcgcttca cctcgtccaa tgtggcgtcc
     1621 ttgcggccgt agcggatgtt gtccgcaatg ctgccgctga acaggtgcgg cgtctgcagc
     1681 acatagccca gcgcggagtg cagccagagc tggctgcgct cacggtagtc cacaccgtcg
     1741 atgagtacct ggcccgcggt cggctcgtaa aaacggcaca ccaggttgac gatggtgctt
     1801 ttgcccgcgc ccgtctcacc caccagggcg atggtctggc ccggctctac ctttaaattg
     1861 aaatcgcgca gcacggtctc gcctgttttg taggcgaacg tgacgtcccg gaactccacg
     1921 ccgcccgcga tgggaggcca gttcccgcgc cgtggcgcaa aagcggtgcc gtattcggct
     1981 tccacctcgg ggctgtctac gatatccggc tgggtgtcca gcaggtcgat gacgcgctcg
     2041 gcgctggcct gtgcggactg cgtcagctcc cggaactcgg cggcactggc ctcctcgcgc
     2101 acgagggtct ttgtggtcat ggcgcccatg atgccctcgt tgaacgcgcc tgtgatgcgg
     2161 ctgttcgttt tgcgcacggc gcgctggtat ttgaggatac gcttttggaa aaacacgcac
     2221 agcgcggcca gaacgggcgt gatgcacagc accagcagac ccagcttcca gttcatggca
     2281 agcagcacga ccacgatgcc cacggcgaac acgcccgccc acatcaggtc cacaaggctc
     2341 caggcgatca tatccgacag ccgggcgata tcactcacca tgcgggccat cagccagcca
     2401 acggaggtgg tgtcgtaaaa gctgaacgaa agctgctgga gctttgcaaa cgcatcctgc
     2461 cggatgtcgt agctcatgtc catttccagc cggcccgcgc cgcgcacaaa caggaacacg
     2521 cccaggctct ggacgacgac cagagcggca tatacaaggc cgtacagcca aagcccctca
     2581 gtggtgccgc cctcgatgaa atggtcgatg gcatagcggg agagctgcgg ataggcaagg
     2641 tcgatccccg ccaccaccag cagcacgagc atcacggaga ccaccgtgcg ccggtggcgc
     2701 atggcatagg cgatcaggcg tttccacagc cgcaggtcga tcttctggtc ggcagtgtct
     2761 cttttgtttt caaaatcatt ctgtgccatt acgcctcgcc tcctttcggg gacgccgcct
     2821 gcggcagctc gttctggatg gcgcacacgc gccggtacag cccctcctgc gcggccagcg
     2881 tgtcatgcgt gcccatctgc accacgcggc ctttgtccag cacgaggatc aggtccgact
     2941 tacggatggt tgcgatgcgg tggctgatga ggaacatggt gccgtggcgg cgggaatgca
     3001 gcgcgtcgcg gatggcggcg tccgtctcag cgtctacggc ggaaagggaa tcgtcaaaga
     3061 tcaggacagg ggtatcctgc agcagcatgc gggcgatggc cacgcgctgc ttttgtccgc
     3121 cggaaagcgt cacgccgcgc tcgccgacca cggtgtcgta gccctcggag aagctctcga
     3181 tgacgtcatg taccgaggcc gtgcgcgcgg cggcatatac gtccgaaaga tccgcgtccg
     3241 gccgggcgat ggcgatgttt tcgccgatgg tgcggctgta caggaacggt tcctgcaaaa
     3301 cgatgcccac gttgcggcgc aggtgatggc gttcgatgtc gttgacgtca acaccggaaa
     3361 gcgtgatgcg tccggcggtg acggggtaca gccgctgcag aagctgcacc aggctggatt
     3421 tgcccgcgcc cgtgctgccg aggatgccaa tggtctggcc gggccgtacg gtgaacgaaa
     3481 catgggaaag cacgggcacg ccgtcgccgt aatcgaagca cacgtcctcg aaagcgacgc
     3541 tgccgtcgat ctgcggcgta agggctttgc ccggctccgc ttcggcgggc acggcaagga
     3601 tgtcctccaa acgggacagg gaaatatccg ccttgcccag atccgccaga atgcggccca
     3661 gctggcgcat ggggaacgtg agcatggagg tgtaggtggt gaacagcagc acgttgccca
     3721 ggctgaaccc gccgtgcacg gcgaaataga cgccgcacac gccggaaagc gcgatttgaa
     3781 gatagccgat catgtcggag aggccccagt atgcgcccat cagggtggtg aggtgcagcg
     3841 tgatatcgtg gtaagccttg ttgcgccggg tgaatttgtc cagctcagag ctttcctgtg
     3901 caaacgcgcg caccacgcgc acgcccgtca ggttttcctg cagcgtggtg gaaagcgcgc
     3961 cctcggcttc atccgagcgt gtgaactgcc gctgcacgcc gcggaaatag aagaagctga
     4021 acaccacaag aatgggcagc aggcaggtgg atacggccgt catcgcgccg ctgatgggca
     4081 gcatgatgga aatggccgtg cacaccatca ccacggtgcg caccacctcc atggcctgca
     4141 tctgcacaaa gcggcgaacc gtctccacgt cgctggtgca gcgctggaca aggtcacccg
     4201 tctgcacgcc cacatgatag ctgtatggca cggcctccag atgcgtgaac agcctgtcgc
     4261 gcaggcgctt ggccagtccc tcgcccgcaa aggcgatgca gtggccgcgc agataggtga
     4321 atacgccgcc cagcagcgtc accagaacca caagcgaggc cagcacccag atgtggctcg
     4381 agaaatacgc ccgcccgccc atgcgctgga acagagcgga cagagcggcg ggnnnnnnnn
     4441 nnagcggcgg gcagctccgg cgttttgtcg ccgatgacgc tgtccaccgt gaaggaaacg
     4501 acgagcggcg tgacgtaggt gagaaacgtg gcgcacagcg tcgccgccac gcccaggata
     4561 agataccgct tcacgccgtg aaaaatgtcg cccagcagcc ttcgccggaa gccgcggggc
     4621 cggggatggg tctgttcggg ttgcatgatt actcctttgg cgttccccgc tccgccgctc
     4681 acggtgcgcg ggagggagaa agcgccggtg ctggggcgca gccgcacgcc cggtggataa
     4741 tgcgttcagg cgcgcggaag gtaacggcac tccgcgcgca tttatataaa caggccggca
     4801 agcggcacgc ctttacagcg cttttgcacc gggtctggtg caaaaggccg ccggacagcg
     4861 cctccggctg ctgcatctgc gatacaattt gtaataaagt gatttaattc gacaaaaagt
     4921 acagaaaagg gggagcggcg cgggacggcg ccgcctgccc tgcggaacag cagggcaacg
     4981 cagcgcacgc ccgggcgcca ctcccaagcg taacgattca gttgtaagtc cccggaacac
     5041 gtccgcccaa aagggcggcc cgctcaccgg tcacgagcgc aggtctttat actgctcaca
     5101 ggtacatttt ttaaactttt tgccgctgcc gcagggacag gggtcattgc gtcccggctt
     5161 ggcgatcttc ttggccggct gctttttcac gctgccgtcg tccgcgccgg attcacccgt
     5221 gggctttgcc acctgttcgc gctggagagg ctgctcggtg cgcagctcca tggtgagcag
     5281 catgcgggag gtgtcctcgc ggatggacgc caccatctcg tcgaacatgt tgaagccctc
     5341 gatacggtat tccaccacag ggtcgcgctg gccgtagctg cgcagataga tgcccttgcg
     5401 cagctcctcc atggcgtcga tgtgctccat ccacttggtg tccacggtgc gcagcagcac
     5461 gacgcgttca aactcgcgca tcaaaggcga gccgtagcgt ttttccttgt ccgcgcagat
     5521 ggcgtcggcc cggtccgtca ggaccttcac aacgtcctcc tgcttcagct cgcccagagc
     5581 nnnnnnnnnn cccgccagga acagctgggc attggccgca atggactcgt ggatcatggt
     5641 gtgaacattt tcggatacat cctcgccgcg cagtaccttg ccgcgctggc cgtagatgat
     5701 ctcgcgctgg ctgttcatca cgtcgtcgaa ctgcagcacg tttttgcgga tggagaagtt
     5761 gcgggcctcc agctttttct gggcgttctc gatggtgccg gagatcatct tgttctcaat
     5821 gggcatgtct tcctcgacgc ccagggaatc catgatggtg ctgacgcgct cgccgccgaa
     5881 caggcgcatg aggtcgtcct ccaagctgat gaagaagcgg ctggcgccgg ggtcgccctg
     5941 gcggccggca cggccgcgca gctggttgtc gatgcggnnn nnnnnnngca cacctcggcg
     6001 gcttcttccg cgatgacttt tttgtgcgcg gcctccagat cggcatagcg cgcgcgcacc
     6061 tttaaaattt ccgggtcggt ggtatcggcg tggccggtgc tctccacgat cagttcctcg
     6121 gagaagccct ccctgcgcag gtcggctttg gccatgtgct cggcgttgcc gcccagcatg
     6181 atgtcggtgc cgcggccggc catatnnnnn nnnnngctac gatctcagct tccttttcgt
     6241 ggtgcttggc gttcagaacc tcgtgcttca cgccgcggcg gctcagcagc ttgctcagca
     6301 gctcgctctt ttcaatggaa atggtgccca ccagcacggg ctggcccttt tcatggcact
     6361 cgatgatctg ctcgataacg gcgttgaact tggccggttc ggttttgtac accacgtcgt
     6421 tgtagtcggt gcgggccacg ggcttgttcg tgggcacctc caccacctgc aggccgtaga
     6481 tctcctcaaa ctcggcggcc tccgtctgtg cggtgcccgt catgccggag agcttttcgt
     6541 acatgcggaa atagttctgg aacgtgatgg tggccagcgt gcggctctcg cgggcgacgg
     6601 tgacgccctc ctttgcctcg atggcctggt gcagcccctc attgtagcgg cggcccagca
     6661 tcaggcggcc ggtgaattcg tccacgatga tgacctcgcc gtcgcgcacc acataatcca
     6721 cgtcccgctt catcacgccc cgcgccttga tggcctggtt gatgtgatgc tgcagcgtca
     6781 tgttcgcgcc gtcggccaga ttttcaacat tgaagtactt ttcagccttg gcaatgccgc
     6841 tggcggtaag cgtggccgtc ttttttttct cgtccacaac ataatcgccg tccacctgct
     6901 cgtcctggtc ctctttgtcg tccagctcgg ccacgacggt ggctttcagg cccttggcaa
     6961 aattatcggc cagcttgtac agatcggtgc ttttatcgcc ctcgccgctg atgatgagcg
     7021 gcgtgcgtgc ttcgtcgatg aggatggagt ccacctcgtc cacgatggcg taataatgcc
     7081 cgcgctgcac catctgctct ttgtaggtga ccatgttgtc gcgcaggtaa tcgaagccga
     7141 attcattgtt ggtgccgtag gtgatgtcgg cgttgtaggc ggcgcgacgt tcctcgc
//
LOCUS       DAWBSB010000108         7176 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_2057_length_7176_cov_3.343210, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000108 DAWBSB010000000
VERSION     DAWBSB010000108.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 7176)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7176)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7176
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2057_length_7176_cov_3.343210"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    676..685
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1448 a         2307 c         1931 g         1480 t
ORIGIN      
        1 cgccaaagga aaaatccact gttcctgccc catgtgccgg gcaaaatccc gtgatgagca
       61 cagccatagg gacaaaaagg cttttttatc cgccaaacag caaatggatg cgtgacacac
      121 acgccgaaag gaggagcacc gggtgaaaga tacaatacaa aaagaactgg ctcaaattga
      181 acaaacacaa aatgtgcgga tcttgctggc ggtcgaatcc ggcagccggg catggggctt
      241 tgcctctccg gacagcgact atgatgtacg gttcatctac gtccgcccga aagatgcgta
      301 tttgcggctt caaaaacacc gtgatgtgat cgaattgccg atcaacgacg cgctggatat
      361 caacgggtgg gacctcacca agacgctgcg gcttctgcat aagtccaacc caacgctgtt
      421 tgaatggggt gcctccccca ttgtatattt agaaacagat tttgcggcac ggtttaaatc
      481 tgttatgggg cgatattttt cttcgaagag agggctgtat cactatatcc atatggcggc
      541 cggcaactac cgcgaataca tcaaaggcga tatgataaag gcaaagaaat atttttatgt
      601 cctgcgcccg gtcctcgcct gccggtggat ccttgacaaa ggctccccgc cgcccatgct
      661 gttttccgaa ctgatnnnnn nnnnncttgc accggaattg aaaccggaag tcgagcgtct
      721 gctcgagctc aaggtgaatt cccctgaaat caggatgatt ccgcagatca ggacgctcaa
      781 cgaatatctc agttccagca tagcggaaat cgaacaaaaa atcgcgcgtc ttcccaatga
      841 gcgggaaatc ggctgggaag agctgaacga actgttcctg tcgcagctta tatagtttgc
      901 agcagcgctt tctttggcct aagccaattt gttttcaatg cggcgggcaa aacatgtacc
      961 gttattccaa ttaaaacagc ggccggagca aaatgctccg gccgctgttt ttgcaatatc
     1021 aagcagtatt ttccgccatc caacagccta ttcccggccc tcctgtaatg caggccgtat
     1081 tcgagccctg cgggtttgcg cccatgcaga cggccatggc ctttttcttc ctgctgacgc
     1141 cgcccggaat ttcaaagcat tttttgctcc accgaatgga gccgttcagc ctacgttccc
     1201 tgtctcacgg tattcgcgcc agacattttc aaagaaatca tcatccttcg gaatggggcg
     1261 gacgggacgc cattcaaaca cacatgcgcc gtcccggtac agcatctcct gcacccggcc
     1321 gccccggctt ccgtcatcca gcacaaaggt aaaggcgtca ggcagctgtg cataaggctt
     1381 tttgcccgca gggtctgtat acagggcgct gccccggctt tttcctccct gctgcatata
     1441 atccagcata gccgccagat aggcatactg gctgatgagc atatcgcgca gacggtagac
     1501 ccagcgcagc tctgcggcct ccgaggccgt gacgttccgc tcaaaggagg caagctgagc
     1561 cttcacctcc gccagataaa cggcaatcgc cccgccgtca cgaatggccg cgccgcattc
     1621 gctcatgcgc cgggccgcct gtgcccataa tgcacgcaca ttgccctgcg gctgcagggc
     1681 gcgacgcgcc agcgcgccca tctgctccag cgcctgtgcc gctgcctgct cgaatccctc
     1741 ttccggcgca ccgccgcgct ttgcagcaat atactgcgct gcgcgggtgc ttcccacctg
     1801 gcccgcgttc agcgccgtgc ctccgggccg gtacacgcca tggctggcac tcgcttcgcc
     1861 cacggcaaaa agccctgcta cattcgtctg ccaccagcag tcgatggcga ggccgccgtt
     1921 attgtgctgc gcgcacagcg agatctcaag aggctcacgg gcaaggtcca cgcctttgtc
     1981 ccggtaaaat tccacggccg gagcgttcat gtggccaaga cgctcgacgg gcgtgccgaa
     2041 gcacgcaccc gcccgggtca gataatcgcg cgcctccggc tccagttcat cgtaagaaaa
     2101 cgcgccgccc acagggttcc ggcggtaatc cagatacacc cgccgcccct tgcagcgttc
     2161 cagatagacc agaacgtcga tgatggagct tccccccgcc accttccgca catcgaacgg
     2221 ccactgatac cccttcagaa acagtttgct gagcatctcg gcaggtgttt taaaaaagtc
     2281 catcaggaat tcccgctcgt ccgagccgtc ttccgcggcg gaatagacgc ggggaagcac
     2341 ctgcatgtag gtccccgata cgttccaccg cggctttacc gacgccagac catattgcca
     2401 ctcggtcagg ttttttcctt tcaccccggc ctccagcgca aggcctgtgg ccccgtagtg
     2461 tccggcggga tacacactgt cagcgtacat accggccggg cctccggtag cgtatacaat
     2521 gtttttgcat tgtatcagga cgaaacggtc tgtttcctcc cgcgcggcgg tatccaggca
     2581 gagcaggccg tacgccgtat ggccgtcgct gaggatgcgg atgacctgaa gatggtccag
     2641 cataggaatc cccttggcct gcacggcctg ctccaggcac tcggtcatct gcctgctggt
     2701 gtatgggcct acgctggtgg cacgcctgcg cgggtcgtga tctgttttgt agccgatgta
     2761 ttctccgtaa cggttgcgcg ggaacggaac gcccagttcc acaagcttca gaaagctctg
     2821 ggcagaaagc gcagcctcgc acaaagcgat atcgccgtcc acacactgcc ctgagaacag
     2881 cgtttcagcc atttcccgca cactgtcgcc ctccgggccg gaaagtgtaa gcttataata
     2941 ggtctgcttg tcgctgcctg tattccgact ggttccggca aggcggttct ccgtcacaat
     3001 gaggatatcc cgctggccca gctgccacag ccggtcagca gcattgtagc ctgcggcgcc
     3061 tgttcccacg acggccgtgt tgcatcggca gagccgggcc gagaagcccc cggcggaaaa
     3121 gcgttcctcc aacatggcgc atcctcctta caggcgtttg atgtgaaaac cgccgtccac
     3181 atcgatatag ttgcccgttg tatacaggaa tttatcgctg cacagcgcgc tcacggcatt
     3241 ggccacatcc tgcggagtgc cccagcgggc gatgggaaac attccgtccc cgatcatctt
     3301 atcatatttg tcctgcacgg cgctcgtcat atcggtggcg atgacgcccg ggcgcacttc
     3361 gtgtaccaga atgccctcct gtgccaggcg gtcggcgtac agggtcgtca gcatggagac
     3421 gcctgccttt gatacacaat actcgccgcg attgacggag gataccaccg aactgcagct
     3481 ggagacgttc acaataacgc ccttccggcc atccagcggc gcctgacgga tcatctgctt
     3541 cgctacggcc tgcgtcagga acatgttccc cttggtgttg acgcccaaaa cataatcaaa
     3601 gctctcctcc gacatttcca gcaggtccgc gcgtacacgg ggcgccacac cggcgttgtt
     3661 caccagggcg tccacacggc cgaaggcagt gagcgccgca tcaagtatcc tgagcctgtc
     3721 gtcgtggtcg ccgatattcg cctgtacata agcgcagcag acgccctgtt tttgcagcgc
     3781 atccacggcc gcctggtttt tctcctgcgg gccggtggcc accatcacga tattgtagcc
     3841 gtccaggccc agctgccggg cgattgcaaa cccgatgccc cggctggagc ctgtcacgat
     3901 cgctgtcttt ttcatctttt cttctccttt tcccctgccc gggccgctca cggttttaca
     3961 ataaccttaa tgctgttgct gccgcggtcc agcgcagtat tcaacgcctg ctgcagctgc
     4021 tcgagcggga aagtatgtgt gacgaatttt tccaagggca gatcccgcgc cgccgcaatg
     4081 gcgatgggga aatcccagca gtagccctgg gcgccctgaa tgcgtatttc atgcgttacc
     4141 aggcggctcg ccgggaatgt gacctgaggc ttttcgtaaa ttcccacgac cgtggccgta
     4201 ccgttgcgcc gcagtgtcat aatcgcctgc aaaaagcagc tttcgcggcc cacacattca
     4261 aaacttttat ccacaccttt tccgcccgtc agacgggcca cttcctgctc caagtccgct
     4321 ttgccgctgt ttattgcaat ggtggctccc acctgcaggg ccatctccaa acggctgtcg
     4381 gaaaaatctg cgatgatgac ttgcgccgca ccgctgcggc ggcacattgc cgccaccatc
     4441 atgccgatgg cgcccgcgcc gatgataagc accgtctccc caagcgctac ctgcgcccgg
     4501 cgtaccgcgt gagtcgccac ggacagcggt tcgatcagcg cccctgtctc gtaagtgaga
     4561 tattcgggca gctcataaac ataaggagct tttgccacca cataatccgc catcgctcca
     4621 tcgccgttgc ggtaggtgaa gctgatatgc tcacagtagc cgtagtctcc gtggcggcag
     4681 gcatcgcact tgccgcacac aatacacggc tccaccgtga cacgctgtcc cacgcgcaca
     4741 ttgtccacac cgccgcctac ggccgtgacg tcgccggaaa attcatgccc gatggtcacc
     4801 ggaagcggag cggaaggatg cagccctctt gcgatgtgca gatcgctgcc acagatggcg
     4861 ctggcacgta ctctcacaag cacttcgtca tcggccagtg cgtggatctc ccgttccaga
     4921 aaacgcactt taaagggttc gtaaacctcc cccactcggt tgcggatggt atccatgtca
     4981 aatcctcctt cagcaggcgg ggcttatttg cggtgctcgc gcgccactgc ctctgcattg
     5041 tacttcttaa acattggccc gtacagggcg ctgtccttga tgacacagcc cgtacggccg
     5101 tgatcccgca tgatctgggt gcacatactg catgtaacac agcattttcc ggggtccatg
     5161 gccccggtgg tcatcaggtc atgaggcgca tcgggatagg caaaactttc ccgtccaagc
     5221 cccacaaagc tgcagcttcc gtcggccagg ttggccgcgc ctgcataggg caggaactgg
     5281 cgcagccagg tgtagccgtt gcccaccacg ggcacgctgc ccgcggcctg ctggaccgtg
     5341 cgggtgaacg caaacagccg cgccatactc tccagcggat gctcttccgg cgtggggatg
     5401 ccatagctgg atttgtcaaa cggccgcgtc acctgcgggt agatataata agggtttccg
     5461 ccgctgttgg acagaaggtc cacaccgttt tccaccatca tcttcaccaa cgccaccggc
     5521 tccgtggggt cgaatttcca catgtcctcg cggtcgcacc cgaagccata ggggtacggg
     5581 tgcgcgtcga acacattgaa gcggcaggcc acgatgaagt catcacccac agcttcgcgc
     5641 acagcccgga tggtctggcg caaaaaacgg gtgcggttct caaagcttcc gccgtatttt
     5701 cccccgcggg tatggctcgc cagcagttca ctcagcagat aacggtgaca gcttttgatg
     5761 tccacaccgt caaagccgac ctcccgtgcc agcagcgcgc tctgtacata ccgctccgtg
     5821 agcgcgtcga ggtacatgtc tgtgaccaca acttccccgc ctttatccag aagcgggtca
     5881 tgctgcggaa ccagcggacg ggcaatgtgt ccttccgggc ggctgtaacg gcccgaatgg
     5941 gtcagctgca ggatattcac cggcttgtgc tccgcaccat tcttctgcgc cgccgcagcg
     6001 ttggtctttt gcagaagcgc cgcaaacttg ctgcggttgg acgccgtgag catcatctgc
     6061 agttcgttgg cgcgcccctc cggcaccacg gcgcaggctt cccaccaaag caggcccgcg
     6121 ccgcccgacg caaaacgcag atagcgccgc tcaacaagct cgcttggcga gccgtcggta
     6181 ttggaatcgc agccttccat cggcaggacg gccatggaat tgggcgcggt gcgtccgcct
     6241 acctttctgg gctggaacag cgggctcaga tcatcccgga acgagaggcc ggccccaagt
     6301 tcctgcgctt tggcctttac cgcttccagc gttttaaaat caaatttttc atgtgctgcc
     6361 atattgtatg gttagcccct ttcacaatag ggcggggccg ttacagcccc gccttcgtct
     6421 taaaaaatgc ttgcagataa aacagtccct cccgcgccaa ttcgtccggc tctgagtgca
     6481 cctgcaccca actggaatcc agaccgccca gcgcaaaggt ctccatcgtg atatgccctc
     6541 cgtagccgta acgcaccaac agacctaata agtcctccca ggggattact ccctgtccgg
     6601 gcgcgccgcg gttgttcgcg caggcatgaa aatggttcac tttgcccgcc tgcagcacct
     6661 gctccagcgc tgcgcagagg tcccgttcct ccaagcaggc gtgatatgtg tcgaagtgga
     6721 cgcccacatt gggcgcgtcc acttcttcca ccatgcgcag cacctgcccg gcagtattgg
     6781 caacgctggt gcggtagcga ttcagcggct caacagagag taccacgccg taatgttccg
     6841 cacggcgggc caattcccgc agcccatcga cagccagcat ccactcccgt ttcttctcgt
     6901 cgggtgaaag ccagtggcgc tttccgccgc cagtgtacag cgggcccgcc agaatgcctg
     6961 cgcccagctg cccggctgtt tcaagacagg tcgtcagata atccattgtg gatgcgcgca
     7021 cggcggagtc aaagctggaa agatcccggc ccgggcccag agccgcacac aatgaaacag
     7081 ccagccctgt gttccggatc tcatccgcca gcgcggacgg cacctgtggc tgcgtcatgg
     7141 gcagctccac tgccgtaaag cccatgcggg caacct
//
LOCUS       DAWBSB010000109         7133 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_2069_length_7133_cov_2.845437, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000109 DAWBSB010000000
VERSION     DAWBSB010000109.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 7133)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7133)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7133
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2069_length_7133_cov_2.845437"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1212..1221
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5612..5621
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6339..6348
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1372 a         1966 c         2311 g         1454 t
ORIGIN      
        1 tttttgcgcc agatagtcgg tgaacagctc cacaaggctc acgtcacccg tgcggtcgga
       61 atggcacagc agatgggcgg gcttgtagag cgcggcgatg ttttcgtcct cgtacaccat
      121 gcgcagaggt gggcgttttt tatagggaag gggcctggcc gtgggcggcg ctgcggaaaa
      181 aaattcatcg ttgatgtaaa gttccaccaa gtcgcccggg caaaggcgcg tgtccggcgc
      241 gccttttttg ccgttgacct tgatgcgttt ggtgcggaac gctttgtaga gcagcgattt
      301 cggcatgccg gaggtgacgt tttcgacaaa ccgcgagagc cgtgctccgt cctcgttttt
      361 tccggctgtg aaacttttca tgcgcctttt cctcccgtgc tttcaaaatt cgtcctttta
      421 gtatataata atctcaaccg ttcgtaaagg aggaactgct ttgggcgtac atgacgacca
      481 ccgccggcgt gtatacgagc gtttcctgcg ggagggactg gccgggtttg aggagcataa
      541 cgccatggag tttttgctct ttcttgcgcg cccccggggc gatacgaatc cgttggcgca
      601 tgcgctgatc gaccggttcg gcagcctttc ggctgtgttg gacgcgccca tcgaagaact
      661 ggagcgtgtg cccggcgtgg ggcattccag cgccgtcgcg ctcaaattta tcccgcagat
      721 gtgcgcatat tatctggaga acaagacgtc ccacaaagtg ccgcttacaa gcgtagaaga
      781 agccagcgca ttttttatgc cgaaattttt cgcccgcacg caggaagctt tttatatggc
      841 ggctgtggat gaccgccgcg tgctgctgcg ctgcgtgctc atctccgagg gcagcgccaa
      901 cgccacggcg gtttccgtgg cgaaagtggt ctccgaagca ctcaaatgcg gggcgacggg
      961 cgtgatgctg gcccacaacc atccgcgcgg catcacactg ccttccagca gtgatatggc
     1021 tgtcacaagg gagatctacc gtgcgctccg gctggtgaat gtccagcttt tggagcatct
     1081 gatctttgcg gacaatgaat ttctttcctt tgtgcagagc aattatatgg acagtatccg
     1141 tcagagtgtg caggactgag cgcccgtgct ccggtattcg gataaaaatg aaccgcgccc
     1201 cgggtttgct gnnnnnnnnn ncggttttgg catgcggagg gcgggaccgt cagcgcgcag
     1261 gcagaccgtc cagaaaagcg tcgatgttgc gcacggtgcg cacgtctatg taatgctcga
     1321 tgcgttcggt ttgttccagc tcatccgcgc tgccgttgat gcggcgcagc aggcggttga
     1381 gcacgtcgtg ccggtataaa aggtatgcgc ccgcctgaga gcccttttcc gtcaggcgca
     1441 ggcagccgta ccgctcgttg gtcagatagc ctccggaacg caacagcgcg gccattttgg
     1501 aggccgacga aggctggaca tgcagctcac gcgccagcgc ggagatgcgc gtatagccgc
     1561 cggaccgcgt cagacggcag atcatttcaa gataatcctc catggctgtc gtcagcggac
     1621 cggtgtccgc gcggtcatac ccgttctgcg tataaaacag ctgttctgta tccatgctac
     1681 gccccccttg taacgccttc ggaagaaatg ccatatattg tcaacaggga aacaaagttt
     1741 ccccggccta acggctacgg tatatgttta tgaaaagagg cgtctggata tgacacagga
     1801 aggaacgctc accgcgctgc gggaaggcga acgcgcgcgg gtggccgcgc tgctgacaga
     1861 gggcggcatg cgccgccgta tgcaggacat cggcctgatc gagggcacgg aggtggaatg
     1921 cgtgcaaaag agccccgcag gagacccggt tgcctaccgc atccgcggcg cgcttatcgc
     1981 gctgcgggcc gaggactccg cacgcgtgct ggtcacgggc tgctgaaggg atggggggga
     2041 gagaggatgg gactgacaaa ccgttccacg ggcgcgcacg cggcggacgg catgctggcc
     2101 gtcgtcaagc ggcgggagac ggattccgtc gtcgcgctgg caggcaatcc gaatgtgggc
     2161 aaatcaacgg tgttcaacga gttgacgggt atgaaccagc acacgggcaa ctggcccggc
     2221 aaaacggtgg ccagcgcgca gggatactgc gagacgggcg gctggggctg cgtgctggtg
     2281 gatatcccgg gctgctattc tctgatggcg cattccgcgg aggaagcggt ggcgcgggat
     2341 ttcatctgct ttggcggtgc aaacgccgtt gtggtggtgt gcgacgcctc ctgcctggaa
     2401 cgcaatttga atttggcgtt gcaggcaatg gaaattacgg ataatgtggt actgtgtgtg
     2461 aacctgatgg acgaggcgcg gcgcaaaggc gtggaagtgg atgcggccct gctggagcaa
     2521 aggctgggcg taccggtcgt cccggcggca gcccgcagcg gcgaaggcct gccggagctg
     2581 atgtgtgccg tggagcgcgt gattcgggga gaatgcgcgg cgcgcgtgtg ccgggtgcgc
     2641 tacgcacagc ctttggaggc tgcgctggcg ttgctgacac ccgccgtggc caacgccgtg
     2701 ggaacaggcg gcccggctcc gcggtggctg gcgctgcgcc ttttggaagg agacacggag
     2761 ctgaatgccg gaattgtgcg gtatctgggc ttttcgcttg cgcagcatcc ggatgtggcg
     2821 gaaatactgg cgcgggcaag gcgtcttctt gcggaagggg gcgtcacgca ggaggcgctg
     2881 cacgatgaag tcgtgcgcag tcttgtgcgc acagcggaga ccgtgtgcat cggtgcggta
     2941 cgccacacgc ccacgccgca ggagctgcgt gaccggaagc tggaccggat tctaacgagc
     3001 cgccgcacgg gttttccgct catgctcctg ctgcttctgg gggtgttctg gctgacgatc
     3061 accggcgcga acgtgccgtc ccagcttttg gcggacggtt tgttttgggt gggagaccgg
     3121 ctgggcggag cgatggcttg ggccggcgcg cctgtgtggg tgaaaggcct gctggtggat
     3181 ggcgtatacc gcgtgctggc gtgggtggta tctgtaatgc ttccgcccat ggctatcttt
     3241 tttcctctgt ttacgctgct ggaggatctg ggatatctgc cgcgggtggc attcaacctg
     3301 gatcgcacct tcaaaaaatg ccgggcctgc ggcaagcagg ctctcaccat ggcaatgggc
     3361 ttcggctgca acgcggcagg ggtggtgggc tgccgcatca ttgattcccc gcgggaacgg
     3421 ctcattgcta tgctcaccaa caattttgtg ccctgcaacg gtcggttccc cacgctgatc
     3481 gcgatcctga cgatgttttt tgtgggcgcc tccggcggat tcggcgcttc gctggcttcg
     3541 gcggcgctgc tcacggggct ggtgctgctg ggcgtggggg ccacttttct tgcatcacgg
     3601 ctgttgtccg gcacggtgct caaaggtgtg ccgtcctcat ttacactgga gctgccgccc
     3661 taccgcaggc cgcagatagg taaggtgatc gtgcgttcca ttttcgaccg cacgctgttt
     3721 gttctgggcc gcgcggcaat ggtggccgcg cccgcgggca tgcttatatg ggtcatggcg
     3781 aatctcacgc tggacggcca aacgctcctg acgcgcagcg cggcgttttt ggacccggcg
     3841 gcccgctttt tcggcctgga cggcgtaatc ctgctggcgt ttattctggg cttcccagcc
     3901 aacgagatcg tcgtgcccat catcatcatg gcgtatcttt caacaggcag tctggtggag
     3961 atgaacgatc tttcggcgct gcatacgctg ttggtacaga acggctggac atgggtgacg
     4021 gcggtgtgca cgatgctgtt ttcgctgatg cactggccgt gttcgacaac atgtctcacc
     4081 attcgcaagg aatcgcagag ctggaagtgg acggcagccg cgtttgcgct tcccaccgcc
     4141 gccggtcttg cgtcctgtgc tctggtggcg aacggcgcgc ggctggttgg gcggctgttg
     4201 ggcggatagc acgtccgcgc aagcgtgctc cggcgtttcg aaaggcaggg cgccggacgg
     4261 gtatgacctg cacgtgcggg gcaaaataac caaaagagcg gatggcgtac gagggaaaat
     4321 aggagggtat aagattgcag ttttctacca aaattggtat aataaaacct ctgggaagat
     4381 tctctggaga atgacgtttt acccgcggct gcattgtatt ttgagggatg gaaatggaag
     4441 aagaacacaa ggacagattt attctcaagg cgccttacaa gcccacgggc gaccagcccg
     4501 aggccatcgc acagctgacg gacgggctgc tgaagggcga taaatgtcag acactcctgg
     4561 gtgtgacagg cagcggcaaa acgtttacga tggcaaacgt cattgcgaac ctcaaccgcc
     4621 ccacgctggt gctggcgcac aacaaaacgc tggctgcgca gctttgtaca gagttcaagg
     4681 agttttttcc ggacaacgcg gtggagtatt tcgtaagcta ctacgattac taccagccgg
     4741 aggcgtatat accaagcact gacacctata ttgagaagga cagtgccatc aatgaggaga
     4801 tcgaccgtct gcgccattcg gccacagccg cgctgtcgga acggcgcgac gttatcatcg
     4861 tggccagcgt gtcgtgtatt tactctttgg gcgaccccat tgattaccgc agcatggtca
     4921 tcagcgtgcg gcagggcatg cacatgagcc gcgatgagct gatgcaccgc ctggtggcgc
     4981 tgcagtacga gcgcaacgac ctcaacttcg tgcgaaacaa attccgcgtg cggggcgaca
     5041 ttgttgagat caacctcgcc tatatgaacg atgtggccat ccgtatcgaa tttttcggcg
     5101 acgaggtgga ccgcatcagc gaagtgaacg cgctgacggg cgagcgccac gcggtgattc
     5161 ggcatattgc cattttcccg gccagccatt acatcgtttc aggtgataaa atgcgcgcgg
     5221 gccttgcaaa tatccgcgac gagctggacc ggcaggtgaa ggccttcacc ggagagggga
     5281 agctgctgga ggcccagcgc attgcccagc gcaccaatta cgacattgag atgcttaccg
     5341 aagtgggcac ctgcaagggc atcgagaact actccgctgt tttgtcgggc cgtgcgcccg
     5401 gttccacgcc gacaacattg ctggattatt tccccgagga tttcctgttg tttgtggacg
     5461 agagccatgt gatgctgccc cagctgcgcg ccatgtacgg cggcgattac gcccgcaaaa
     5521 aaagccttgt ggacttcggc ttccgcctgc cgtcggcatt tgacaaccgc ccgctcaaat
     5581 tcgaggagtt tgagtcgaag ctgaaccaaa cnnnnnnnnn ncgcccggcg attttgaacg
     5641 cgcacagtcc acacagatcg tggaacaggt catccgcccc acgggcctgc tggacccgcg
     5701 tgtcatcgtc aagcctgtgg aagggcagat cgaggacctg ctgggcgaga tcaacacccg
     5761 cagtggccgg ggcgagcgcg tgctggtcac aacgctgacg aagaagatgg ccgaggacct
     5821 gacggattat ctctccgacc atggcgttaa ggtaaaatat atgcatcacg aggtggatac
     5881 cttcgagcgt gtggagctga tgcgtgatct gcgcaagggc accattgatg tgatcgtggg
     5941 catcaacctg ctgcgcgaag ggctggatct gcccgaggta tcccttgtcg ccatcctgga
     6001 cgcggacaaa gaaggctttc tgcgcagtga aacgagcctc gtgcagacca ttggacgtgc
     6061 cgcgcgcaat gcggacggcg tcgttatcat gtatgcggat tccatcacgc cctcaatgga
     6121 gcgtgctatt atggagaccg agcgccgccg cggcatccag gaggcctaca acgccgccaa
     6181 tggcatcaca cccaagacca tcgtcaagga aatcagcggt atgggcgtgc tggagatatc
     6241 ctccaaagac gacagtgcgg acaagcccgc caaacgcatg agccgcgcgg agatcgagca
     6301 gaagatcaat cagctgacgc gggagatgcg ggaagcggnn nnnnnnnnca tctacgcaga
     6361 cggacagcgc cggtacatgg aaagcctgtc cagctatgcc cgccagtttt tgggccagat
     6421 ggagaagcct gatgtggacg agatacaggg cttgtcgccg gccatatcca tcgaccagaa
     6481 aacgacctcc cgcaacccgc gctccacagt gggcacggtg acggaaatct acgattacct
     6541 gcgcctgatg tatgcgcgta tcgggattcc gcactgtccg gtgtgcggcc gcgtcatcag
     6601 ccagcagacg gtggaccaga tggtggacga ggtgctgcgc ctgcccgagg gcacgcggtt
     6661 ccaggtgctg gcccccgttg tgcgcgggcg caagggtacc cagcagaagg agttcgaggc
     6721 cgcgcgccgt tccggctttg cccgtgtgcg cgtggatggc aatatgtatg agctggacga
     6781 ggagatcgcc cttgaaaaga acaagaagca cacggtggag gtcgtggtgg accgcctcgt
     6841 cgtcaatgat gaggtgcgca gccgcctggc ggattccatc gagacggcca tcggtttgac
     6901 gggcggcctt gtgggcatcg acgtcatcgg aggcgatgag atgctgttca gccagagcta
     6961 tgcctgcccg gagcacggca tttccatcga cgagcttgcg ccgcgcatgt tctcgttcaa
     7021 caatcccttc ggcgcgtgcg agacatgcac gggcctcggt acctttatga aggtggaccc
     7081 ggcgctcgtt attccgaacc gcagcctttc catccgcgag ggcgcgatca agg
//
LOCUS       DAWBSB010000110         7086 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_2082_length_7086_cov_3.158015, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000110 DAWBSB010000000
VERSION     DAWBSB010000110.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 7086)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7086)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7086
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2082_length_7086_cov_3.158015"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2585..2594
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3104..3113
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1398 a         2256 c         1947 g         1465 t
ORIGIN      
        1 ggatggaggc ctcctctgcc accatatccc gcccgacggt ggaaaggtaa tcgtcagcct
       61 tattgcgctt gtgaagcgcg aaagcctcgt gccggtacag catttcgccc acggtgcgcc
      121 agccgccctg ggccatgcgc cggcggtttt cgttcacggc ggcaagaagc cgcccgtcct
      181 cgccgtccga ggcggttcgg cggttgcttt taaacccgcg gcgctgggca aggtgcagca
      241 aaatgcgcga aaattcggcc ttgccgaccg gttcgtccaa cgcgcgcgtg cgcagcgcat
      301 agatgtcttc caggccgccc gcttcaaaca tttgcgcgag cgcttcccgg gtggaaaggc
      361 cgctgcgttc cagcagggcg taaacgtccg cacggcgcag cgccttgcgg cgcagacggc
      421 ggcgcatccc gcgcgccatg cgccgcggcg cggcaaggct ttcgcccgtc ttgggctgct
      481 cggccttgtc gaaaatgcgt acacccagac ggtgcagtcc aacaggttca cccgtgccgt
      541 tcaaaccgac gacggcccag ccgacagacg ccacgccgat gtccagcccg atgccataca
      601 atgtgttttt catattaccg cctctttcac cagcatttgc atgaaaaggc cctgcttttt
      661 acgcttccat ttatatcatg accgaattac gttgcagccg tcaatcagca cagtggataa
      721 aaatatccgt gtcttttggg caaaaaagat gacgccccgc tgaaagcgag gcgtcagagc
      781 tttacggtgc taagacctta tcatagcaac cataacagtt cttggtatgg tataagaact
      841 ggtttcagta tagcagttgt ttgcaggaat gtaaagcctt cgacacgaaa attttttgcc
      901 ggttgtgctt acaaggaggc gccgcgcgcc aaggcgcgcc ttggaaaagc cgggccgcct
      961 tggcggatga aaaaacaaga aggcggcgca gcgggcgtat gagccgccaa tgccaaaaga
     1021 ccgcaaagaa tcaatgttct ttgcggtctt ttggctggtt gggccagctg gattcgaacc
     1081 agcggaatgc gggagtcaaa gtcccgtgcc ttaccacttg gcgatggccc attattgcag
     1141 aacggcaaat ccgcatgcga atttgccgtt cctgatttta aatggggtgg gagatgggac
     1201 tcgaacccac gacaaccaga accacaatct ggcgctctac caactgaact acacccacca
     1261 tatttggtgc gcccaaaggg actcgaaccc ctggcccact gcttagaagg cagttgctct
     1321 atccacctga gctatgggcg caaatgtaag ccacgatcgg gacactgctt ggatatttta
     1381 caataagacg accggagttg tcaagggtat ttttgaagaa aaagcatatt tcatgaaaaa
     1441 atcttcaacc gcttgacggc atgggccgtg acgcgataaa ctgttaggcg gaaccgtgtg
     1501 aaaataaaga acggaggatc agaaaatgga acttgcattg caacaggaaa aagcaaagca
     1561 gctgcgggcc gacatcgtga agatgacgta tctcgcaaaa tccggccatc cgggcggctc
     1621 gctgtcttgc gtggagatgc tgatggcgct ctattataat ataatgaagg tggacccctc
     1681 caacccgggc tgggaagaac gggaccgctt cgtgctcagt aagggccatg cctgccccgc
     1741 gctgtatgcc gttctggcgg acaaggggta tttcccgcgg gaggacctct ggacgctgcg
     1801 caagttccac agccatctgc agggccatcc cgatatgcgc aagaccccgg gtgtggacgc
     1861 caacaccggc tcgctgggcc agggcgtttc cattgccggc ggcatggcgc ttgccgcaaa
     1921 gtataagggc gagagccacc gcgtgttcgt catcaccggc gacggcgaga gccaggaggg
     1981 cctggtgtgg gaggctgcca tgtccgccgc ccactataag ctggacaacc ttaccgttct
     2041 gctggaccac aacggcctgc agatcgacgg caccaatgac gaagtgatgg gcgtgggcga
     2101 tatgatggcc aaatacgagg ctttcggctt cgcgtgcgta aaagtggacg gccacgacat
     2161 cggcgccgtt gtggaggcgg tgaacacgcc gcacccgggc aagccgctgt ttgtctgctg
     2221 cgaaacgcac aagggccacg gcgtgtcctt catggaagac caggtgggct ggcacggcaa
     2281 ggcgccgaac aaggagcagt atgagcaagc gatgaaagaa ctgggggtat ccgacaatgg
     2341 ctgagagcaa atccctgcgt attgcctacg gcgaggcgct ggccgagctg ggcgcggaga
     2401 acgacaagat cgtcgtgctg gacgcggacc tttccaacgc caccatgacc aaccgctttt
     2461 ccgcggtgta tccggaccgc ttttttgatt tcggcatcgc cgaggccaac atggcctgcg
     2521 cggccgcggg ctttgcccac gaggggctga tccccttcat cagtacgttc gcgctgttcg
     2581 gcgcnnnnnn nnnncttacg agcagatccg caattccatc gcgtatgtga actccaatgt
     2641 gaagttcggc ttttcccatt ccggcctgtg cgtgggcgag gacggcggca gccaccaaag
     2701 catagaggac atcgccctga tgcgcgtgat gcccaatatg acgatctttg tgccctgcga
     2761 cgccgttgag gtgcgcaagg ccgtgcgcgc ggccgcggcc atcgaaggcc cggtgtatat
     2821 ccgcgtggcg cgcccggtgt gcgaatgctt caccgacgag agcacgccct ttgtgcccgg
     2881 aaaggcgaac atcatgcgcg agggcggcga cgtggccatc atcgccatgg gcctgatggt
     2941 ggccgaggcg ctgaaggccg ccgacctgct ggcggcggag ggcatccggg ccactgtggt
     3001 gaacatgcac acggtgaagc cgctggacga ggatgcggtt ttgtccgtgg ctgaaaaatg
     3061 cggcgccgtt gtgacggccg aggagcattc cgtcatcgga ggcnnnnnnn nnnccctaca
     3121 ccgtcaacgg cgggctgggc tccgcggtgg ccgaggtgct ggcgggcagg tccggcgcca
     3181 aattcgcgcg cgtgggcatc gaggaccggt tcggccagtc cggcacgccc gcggagctgt
     3241 ttgcagacta cggcctgaca gcggagaaca ttgccgaaaa cgcccgccgc ctggtgaagt
     3301 aagcgcgaaa aaacaggttg actttgtttc cgtttattcg gctttttcgc tggattctta
     3361 caaaaaaatt acagtttgcc attctttccc gcaaaaccct taaatttaca aatatttttc
     3421 ctgaaaactg caacattttg gccctctgcg tcgtatacta agcagagggc acaaaaagca
     3481 gtgtcccgga ttgaagcgtg gacggcggcg ccaaaggcgc agccgacgga aaatatgcgg
     3541 aaagggcgtg tggaatttat gacgacttcg atcacttctt ttatggacaa cctcaaacag
     3601 atttttgggc tggtttattc agacacgccc accgaatcct acgaggaaag cgaagagtac
     3661 gaggactgag ctttgtgcga aagcgcagcc ggcctttata aaagcgtccc gggcggtttt
     3721 gccgccaacc tctgagggac accggacgct ttgtgctgcg cagccgcttt ccggtttccc
     3781 tcagttctcc tttccggcgc acaaaagggc aatttcgcgc acttcgcaca caaaattgcc
     3841 ttttgtacgg tagaggtatt tgcccgcccg gcgcatgtcc gggcgggcaa acggtttttt
     3901 aactttttcc tgcggcggcc cccacgttac ggcgaaacgg ctcttacggc gtcagccgta
     3961 acttgtgcgg cgccgcccta tgggcgcgtt accagccgca gatattcccg ccccatctgc
     4021 ggcgggaacc ggtggcgcag gcccgtctgc agcgtcaggt gcacgcgccc gcccgcgctg
     4081 tgcagcgcac cgtacagcat ctgcgccatt tcgatgcagt cgctgtcctg cgtaccgcaa
     4141 aaaagatgca cctctacgtt ttttttcgcc agcgcacggc aaaccgctgc gcagtccttc
     4201 tcaagcagcg gtgtccacgg ggattgcagc gccagcgtgt cgcaggaaag cccggtatac
     4261 agcagcgtgc gcagcagcat atcacagccg gcggaaaagc ccgcccaaac ctgccgctca
     4321 tagcccgcgt cctgcacaat acgcgccgcg tccgctgtct gcctgtaatt ttcgccgtcg
     4381 tattcccagc gaaaccgccc cgtgcaatcc accaccgagg actgcactgt ttccacctgc
     4441 gcacccgcac gctccagcgg cgcccaatcc cggcgcgcaa tcccggcgtt ttgtccgttg
     4501 ccgtgcaccg ccagcaccaa cgttccggca gcgcgcccgc gccacgaaaa ctgcatcctg
     4561 cccgcacgcc gggcctcctc gtaacgtccc tcggaaattt tgcgcagcgt ctcaaaccgc
     4621 gcgtccccga acagcggctt taagtccccg tcgtccagca cctcgggacg gtaccaatac
     4681 tccttttgct ccaccgcctc ggccagccac tccagcgccg caggcgcgcg gcccgccccg
     4741 gcggcaaggc atgcgagaaa ataataaatc tgcggatgct cgccgtcctt ccatgcccca
     4801 cgctgcgcga gcaggaaatc ataggccggt tcataaccgt cctgctccgc caacgccaat
     4861 acttcttcca ataaaacatc ttcgttcatg tgtttatgct ccttttttgg atttaagggc
     4921 ataaaaatgc ccgggagagt gatttctccc gggcaatcac aaaacgaatt tacacaaagg
     4981 acacgccggg agccgcgcct atcgcagaaa attttttcat agatgaccgc tccctttcca
     5041 tacaggcttt ccggcgttgc ctcccaccgg gcctgtgcca aagcacggga aacccgccgc
     5101 gtgcagatta taaaccttac gcgcaccgtt tgtcaagatg cgccgttctt caaaaaacgg
     5161 cccgcgcgcc ccgccgcctt caggcggcgg gttcctcgtc cagttcctgc cgcacgaaca
     5221 cgacctcctc gtcgtcctcg tcgaacaggc aattcaccgt ggggctgtgc agtataaact
     5281 cagtggggct gtattttgct tcgccgttcc acagctcatt atctgtctca atgggtgaca
     5341 tattttcaac tgcgtccctg ggcaaaatca ccacaaagga acctggtgca aaaagaatag
     5401 ctattttctg cactttgcca tccaccacta cttcgccgta gcattgataa ttttcttcct
     5461 cttttacggt aaaccatgcg ccgtattcct cgcataccca tttagcatct ccatagtcgt
     5521 aagggcggca gccactatat gggtcgcacg cgcacagcga aagcacgcac agtagcataa
     5581 tcgccgcgca ccggacggca tgccccaaag ccctatttcg cgttctgcac tctgtacccg
     5641 acaataaatt gtccattttc aataacttcc cccattgtct ttttttccgt tgactccgcc
     5701 gaattattaa atcgattgta aaccatcaat tctccgttca ccttctgcac cgcgaatgta
     5761 tgatacccct caaaaatcgt accctttcgg ttccagatcg aaacgatcat cacatcgcct
     5821 tcgcccgcct cgcgctccag ggcgtccggg tcggtgtatt cctcgtactg cacgccgtat
     5881 tgcttcagca cctgcggcgc gccgtagggg ttcgtccccc atgctccgcc gcccgccatc
     5941 gcgccgctta tctcatagcg ccggcccacc tcggccaggc tggggtcctt ccccagcaga
     6001 tacagcgcgt tataggtccc gatcacgccg cacccgctgt ccttcatccg ggaattgccg
     6061 taggcgaatt ctcccgccgc gtcaacgccc tgcccgtgga cgggccgccc ggagatatcg
     6121 tgcgttttgt ttctttcata gtttgcattg cttgtttcaa tcgcctgcgc aagccctgtt
     6181 cccggctcgc tgccgggaac gctgggaatg ggggacaaag ccgtgctcag gcccagagcc
     6241 gcccgctcca gcaaaggccg gacatatttt gtgtacgccg cgccaacacc ttcggcctgt
     6301 gcgcgctcct gcgccagcgt ctgcgcctgc agcgccgcct gctgctccaa aaaggcatcc
     6361 cgccagcttt ccgtgtcctg cctttttttc agtgtaccat atcccccgcc gggctgcaat
     6421 gctttgccgg tctccgtgcc cgccttccgc cgctcgggcc gcagcagctc cgccagcgcc
     6481 tgccccgcat acaccgcgtc gcggtacgcc gggtccacgc cctcaagcgc aaagccgggc
     6541 gcgccttgct ccggcccggc aggcggctcc ccgcccggcg tggcctgcgc catgctccgg
     6601 cgctgccccg ccaaaagcat ggtccccgcg ccggacggcg ggttctccga cagcgccgcc
     6661 caaggggttc tctctgcccg gcgcgcctgc cccggccacg cttcccccaa gcccggccac
     6721 ggctgctttt cccctgtgta aaaggcattt tgcgctcccg cgccggacgg cgcgaacgac
     6781 ttccgctcct cctccgtaat gaacggctgt tttgccagcc gttcgctgaa cgcctcgtcc
     6841 cagctttgcc acggtttttc ctttcgctgc tgcgccgcct caaacgccgc cgtgtaagcc
     6901 cgcctccgca gcttttcctc caatgccttt ttcttctgtt cctcctggcc catgctccat
     6961 ccgtctgcct ttttcatgcc ggacgcctcc tgcttttatt ttgttttgaa agaggtttgc
     7021 cccttccgct ttccagcata aacctttccg cgtccgtttt tccggaaaac gcaaaggcga
     7081 cgccgt
//
LOCUS       DAWBSB010000111         7046 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_2091_length_7046_cov_3.208411, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000111 DAWBSB010000000
VERSION     DAWBSB010000111.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 7046)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7046)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7046
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2091_length_7046_cov_3.208411"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6191..6200
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1465 a         1904 c         2290 g         1377 t
ORIGIN      
        1 cgtttataca atggacttct atcacgatga gccgctgctt tcttccgcgg tgcagctccg
       61 ctatacaaag ctggccgata tcgcgctgga cgaagcgaac gccgtcaccg tccccttggc
      121 ctgaacggaa aactttttgc agcacggtaa catgccggat acaaaaaggc gacaagtttg
      181 aaacaaacgc gcgctaatct ggaaaaaagg gcaggagcca gcctgtccaa aggggaggaa
      241 tgggaatgca gaccgtttat gatcgggagg tgacgctcca ttcgcggaac gtgcagggcc
      301 ggaatgctct gacggaacgc gccgggcgtg ccgccgtctt ttacaaaccg gctcatgcgc
      361 gcaaaacaca gcccggcgca tggcggcggc ttgccgctgt ggcgctgtgc tgcggggcgc
      421 tgctgtgtgc gttccgtctg tatggaaggg cacgcagccg gttcggagtg cagctcccgg
      481 tcaggacggt cctgcaaaac ccggagctgc ccaacggctg cgaggccgcc agcctggcgg
      541 ctcttttgaa atataagggc gtgcccgccg acaagctgga ccttgcctat ggttatatcc
      601 cgcgcggcga tatcgaagaa accacggacg gccgcaccgg gcctgaccca gagctggcct
      661 atgcgggcga cccggccgcg ggcctgggct tttactgctt cgcacagccg gtggcacagg
      721 gggcgaacgc ctatctggcg gcgcagggct cggctctgcg tgcggcggat ataacaggcg
      781 ttaccggaca ggggcttttg cagtatctgc gcggcggaga ccctgtgatc gtgtggatca
      841 caaaagacct ttccgcaccg cgcacgggag gctgcacctg gctgctggcg gatacgggcg
      901 agacctgcac gccttatgcc aatctgcact gcgtcgtgct tgcggggtgg gacggggata
      961 cctgcaccat cgcagacccg ctgcagggca ggcgcagagt ggacgcggct gcgtttctgc
     1021 aatgcttcag gcaaatgggc agccgcgccg tcgtggtgca ttgaccaaag cggggaaacg
     1081 cggagcgcga aggagcctct gcggaagaaa gcgcctttgt gggatgacgg ctttggcacc
     1141 ggcgcacgca aaacgcttgg cggccctttg tgggccggca agcgtttttt gcagcaaaac
     1201 acttgcgctt ttttgtgtgt tcatgataga atgaatcgct tgcaaatttt cggcaaaagg
     1261 aggcggcatc ttttcatgac acgaaacagc atccggcgta cccagtgctg gcacaatacc
     1321 cggtttcccc gcaggccgcc cccggccgca tccttctgaa cgcacggcca cccgcatgca
     1381 gccgcccgcg cctcgtgcgc aggctttgtt ttgctgtgtg cctttataaa atttagaagg
     1441 atgttttaca tatgacatgg tttattcttt ccctgattgc gattctgttc tggagcggtt
     1501 cggacctgtt ctccaagctg ggctccaggc ccgacgataa atacagccat tggaagatgg
     1561 tcatggctgt gggcgttgta atgggcgcgc acgccttctt tctcatcgcc acgggcacgc
     1621 cgttcagcat ttcggatatc gtcacctatc tgcccgcctc ggctatgtat atcctgtcca
     1681 tgatcctggg ttatgcgggc ctgcggtaca ttgagctttc catttcctcg cccatctgca
     1741 attcctcggg cgcggtagcg gcccttttgt gctttttcat tctgaaggaa acgatgtccg
     1801 gcctgcagtt cttcgcggtg gcgctggtat gcgtgggcgt attcgcgctt tcctatatcg
     1861 aaaagaagca ggatgatgcg ctgcgccgcc gggagggcga tacgcccgac aaaaagcata
     1921 cccgcagctt tctggctatt ctgttcccta tcctgtactg catcatcgac ggcctgggca
     1981 cctttgcgga tgcgctcctg ctggataccg tcattgccga ggagcaggcg aacatcgcct
     2041 atgagctgac gtttctgatg atggccgttt ttgcgtttgt gtatgtggtg atcgtcaaaa
     2101 agcagaagat cagccttccg gcggaaaagc ccaagctggc cgccgccctg tgcgagaccg
     2161 cgggccagtt cgcttatgtg ttcgccatcg gcgcaaacgc catcgtggcc gcgcccatga
     2221 tctcttccta ctgtatcatg tctctggtgt ggagccgcat tttcctcaag gaaaagctgt
     2281 cgaaggcgca gtacgccgtt attgcggtgg ctgccgtggg catcgcgatc ctgggcatgg
     2341 aatgacgaaa ctgtaaactt tttgcaacaa aacggcagcg ccgcgcgtct atatgacgtg
     2401 cggcgttttt gttgtgcgca ggggaaaaag ccgccccgcg cggcacatcg gcgcgccgcc
     2461 gcccattaca actttctaac aagtctgaga gataatgggg atcggaaaaa aggagtgtcg
     2521 ctttatggcg aaaatattga tcgcggagga tgagcgcccc atcagtgagc ttgtcaaacg
     2581 caaccttatg ctggtgggcc acacagcggc gcaggctttc gacggccctt gtgcgctgga
     2641 gatggcaggg gccgagcggt tcgaccttgt gctgctggat gtgatgctgc cgggctgctc
     2701 cggctttgaa gtaaaggaac agctggaccc ggcgctgccc gtcattttcg ttacggcgaa
     2761 gaacaacctt tccagccgtt tgcaggggct gggccttggc gccgacgatt atatcgtgaa
     2821 gccctttgag atattggagc tgctggcccg cgtggaggcc gtgctgcgcc gcgtgcacgc
     2881 cggggccgag gtgttcgacc tgggcgactg ccatgtggag ttcggcacgc accgtgtgtt
     2941 cgttgccggg cacgaggtgg cgcttacgcc gcaggaattt atgctgctgg gcgttttggt
     3001 gcgcaaccgg aatctggcgc tttcccggga aaagctgctg caattggcct ggggctacga
     3061 ttatgaggga gacacccgaa ccgtggacgt acatattcag aagctgcgca aaaagctggg
     3121 ctttgaagat cgtatccaga cggtgtacaa gctcggctac cggctgagca caaaggaata
     3181 gggagcgcgc cgggcgctcc cgaaaatgga ggcgcggcgc ttgcggaaca aaatggaaaa
     3241 gggacgcggg agcgtccggc tgaaattctg gcaaaagacc tatctgctca cactggcgct
     3301 gtttctgctt gcactgggcg gcggcacaac ggccattgcg tccgtcagtc agcagcgtgc
     3361 gtttgaagcc gaatgcggca agctgttggc gcagcagcat tccgtggcgc agagccttgc
     3421 cgcggacgcc gccgcagtgc aggcgcgcag gccggaggcg ctgccgcgtc tggcgcagaa
     3481 ctacgccggg cagctgaaac gctccggcgt ttttctgcgt gtggcgcagg cgggcgacgt
     3541 atgggcggat gaactgccgc agccggattc cgcgttgcct gaggtgccgc cggagggaca
     3601 gcgcgtgcat acggtactgc tgtccggcgg gcggcatgtg ctgtatatct ccgccgtgct
     3661 gcccgccccg ccggaggatg tggccgtcac ctgtgcgttt gacatggagg ccttttttgt
     3721 acagtgggcg cagacgcgcc gggcattttt gctggcaggc gcggcggtgt tcgctgtttt
     3781 ggcggctgcg ctgtacgttg tgctgcgggg gctttcccgc cccatggagc ggctggcccg
     3841 ggtggccggg cggctggcgg acggcgatta cacggcccgc agcccggagc gcacgcggga
     3901 cgaggtgggc cagcttgccc atgcgctgaa tgagatggcg gcccgtgtgg aacggaacgt
     3961 ggccgagctg cgtgaaacgg cccggcgcaa gcagcagctt gtggacaacg tagcgcatga
     4021 gctgcgcaca ccgctcacgg ctgtgggcgg ctatgctgaa tacatccagc gggccgagct
     4081 gtccgaggag gaaaaatacg aggctgcgca gtacatcgtg gaggaggcaa accgccttgc
     4141 cgtgatgagc gagcgccttt tgcaaatggc ggccctgcgg ggcgaacagg cggcgcggga
     4201 gcctgtggac gtgcccgctc tgctggaaaa ggcggcacgc accgtcgcac ccaaagccgc
     4261 tgcccggggc gtggcgctgg agccggtgca ggcggacgcc tgtacggtgc agggagaagc
     4321 ggcgctgctg gaaagcctga tggtgaacct ggcggacaat gctgtaaagg catgcgggcc
     4381 gggcggcagt gtgcgccttt gcgcacggcg ggagacgggc cgcctgacgc tcacggtgca
     4441 ggatacgggc cgcggcatgg acgcggagac gctggcgcac atcggcgaac cgttttacag
     4501 gccggacaag gcccgcagcc gggaacaggg cggcgcgggg ctgggccttg cgctgtgctt
     4561 ccagatcgcg cagagccatg gcgcggcgct gtcctttacc tccgcgccgg gcgcgggcac
     4621 cacggccgcc gtaatattta cagcttgatg acatcttgaa gaaaatgccg caacagtttc
     4681 atgggatact gggagcatcg aaaggaggtt gcttgcgatg aagaaaagca acagaacggt
     4741 gcggaagatg cggagcgctt ggacgggatt tgcggcggcg tgcctggcgg cggcgcttac
     4801 gatgagcgct ttggtgtcct gtgcgccgca ggagagccgg cccgttgtga ccagcgacga
     4861 cctgacgccg cccagcgccg cgcaggaggc cattgcgcag gatattggcg gtggtaaaaa
     4921 ttatgagtat tattgggcgg cagaacatat tgaggaagaa ctggaagcgg tacgggaatc
     4981 aacagaacag agtgtgaagc attggaaaga gattgcagat acaggcaatt attacggaag
     5041 agaagtcagt ggtgatgaaa aagaagagtt gaaaaattcc tttgatgaga tgttaaatga
     5101 aataccactg cttcaggaag aaaagagaaa aatgctggaa agtgagttgg cgctcgatcc
     5161 ggttgtatcg gcacaggagg cagcaaacgc ggcgggagcg gtatttgaaa aaatttatgg
     5221 aattgaactg tcgcaggata ttttgacgtt gtcttgtgtg gaaataaata caaacattaa
     5281 tcccggcgaa gaagtgatac gctcagtatg gaatgtaagt agagaagaag ccgcagacgg
     5341 tgtcttgttc agtaccaatg cagtaggctg tacgttggat gctacaaccg gtgagttcat
     5401 cagtgtagac tatactccca gcaatgatga actaactgaa atgtgggcga cggagttgcc
     5461 gtcatgcttt gtgaaaaaga ctagtattga gactacaggt agctatggct attggaatga
     5521 gaccgaagcg agttatgcag aggtagcgga gaagttaaag cagcaaacga gagaacagct
     5581 ttccggcagt atgttgattg gtggtgcagc agtgacagat gttcgtgtgg aacttctcgg
     5641 aaagatggaa gatagcggag aaaatcaaat aagtttactg gtcgaaggag acaatgggaa
     5701 aacatatcag ctctatagaa actactctat gcaagcatat gtaaattatg gttttgaagg
     5761 gtatccatta cgcgcatatt ggttcagaaa caatacttat ttgaatggaa aataacttta
     5821 cagggccgcg gcccgcaaag ctattcttca aaagcacact ggtcaaaatt taaggtaaaa
     5881 ttaagaggaa catgatacaa tttcgacaca cttgtgtgtt aaactatcaa aaaatggcga
     5941 aaaagggcgg gtgtgcgtgc agacggagta cagaaatatc aaccagggag gccgtgcgcg
     6001 ccgtcccgca cggcatgcgg cgaacgtgcg tgtcccgccc caaagcgggg gacaaagagc
     6061 ggcccgcgtg cagcagccgg ggccttacgg atatccggcc gcgcccggcc cgcgcaccgt
     6121 gcgcggcgca cgtgcggcgg cgcgccgccg ccagaagcgc cgcaggcggc ttgtcacact
     6181 ggggcttgcc nnnnnnnnnn gcctgacgct ggccatcgtg ctgctggccg tgctgcgcca
     6241 gacgtccgct gcccagcccg ccgggacgga gggcggcgcc gccgggcaga gcgccgctct
     6301 gcccacggcc gcggcgcccc atgagggccc gccctatacc attgccatcg acgcgggcca
     6361 cggcggcacc gacatcggcg cggaaggcgt tatcaacgag atccagctca cagaagcaac
     6421 gatcggatat ctggaggggt ggctcaccca ggacgagaat tatacgcccg tgcgcaccca
     6481 cgcggcggat acattttcca aaaacaccga gcgcgcggcc gcggcaaaca gcgcgggcgc
     6541 ttcactgctg ctctccgtgc acggcaattc agacccttat tcctctgatt cctatggcct
     6601 ggaatgctat ccgcagccgc cggggcgcac ccatcatgag gattcgctgc gccttgcgca
     6661 tctcattgcc gataaattcg gcgcggcggg ccagcgcctg cgcggcaacg cgggcgtgcg
     6721 ttacatctac tatgcgggcg acgatgaaag cggctacgaa aagcaggtgg tggaggagag
     6781 cgacagcacc gtttactccg agcagacctt tggccttttg gaaaaaacag attgtcccgc
     6841 cgtactggtg gagcagtgct ttgttaccag cgaggccgat gtggcggcct ggggaacgga
     6901 cgacggctgc aggcgcgcag cccggctgta ttacgaagcc atttgtgaat atttcggcac
     6961 cgagccgctg cccgggacat gatgcaaaaa agcccccgct gcgaacagcg ggggcttttt
     7021 tgcccaaacg actaaaccgt aagccg
//
LOCUS       DAWBSB010000112         6988 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_2109_length_6988_cov_2.332612, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000112 DAWBSB010000000
VERSION     DAWBSB010000112.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 6988)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6988)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6988
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2109_length_6988_cov_2.332612"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3002..3011
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6179..6188
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1350 a         2230 c         1956 g         1432 t
ORIGIN      
        1 ccggaaaatc tgcaagcgag gcgacgcttc aggcgtcagc cgtgaattgt gcggccttgc
       61 cttaggccat acgccgggcc ctccggcgtg ctgatgtaag aaaacacgcc tttgtgccgc
      121 ggccgctgcg gtgccggtcg cccggcgctc aatcataaaa cgagagacgg ggcggaaaac
      181 tttccgcccc gaggattttc cgtgcggcgc gcccttttct tttttgtccc cgccccaaca
      241 gccgcctgcg gcgcaggccg gcaaaatcag cccctccggt tccgtcacgc ctttttgtaa
      301 ttcaaaataa gaattcccgc acacacaagc acaagcgtaa cgaccaaatt caaggcggaa
      361 acgccgtttt gctccgtaag cagcaaattg gaaagcagaa cgcccataac gggggtcata
      421 aaactgtaaa cagtgaccct ggaaaccggg ttgtttttca ggagcacgcc ccaaatggca
      481 tatgccgctg cggagaggaa cgataaataa gttaaaacgc ccagcgcctt tcccccgctt
      541 acaacgatct tacccccaaa cacaatgccg atcagcacca ttgcaatgcc gcccataata
      601 aactggtatc cgcttatcac aacaggattt tcatctttgg aatatttttt catcaataca
      661 gaggaaaagc ccagtgcaac agccgaaaaa agcacaaagc agtctcccgt aaaattcatg
      721 gtaaaatcca aaccgtttaa attcacaaag atgattcccg caaagcctga aatacagcct
      781 atgattttcc cgggcgtcaa tttttcctgt ttgaaaataa ggcttgcgat cagcagcgaa
      841 aaaaacgcgg aggtcccgct taaaatcgtg ccttttacac ccgaggtatt ggcaagcccc
      901 acgtaaaaaa agatgtactg caaaaccgtt tgaaaaacac ttacctgtgc aattttaccg
      961 atattggctt tttgggggta cagcagcttt ccgctggcga tgctgtagat caaaacagtg
     1021 ataacgcccg caaagaaaaa ccgcaccccg gcaaataaaa ttgtggacgg cacgtttttt
     1081 tccggcagga tcagttcata gcctattttt ataaaaggcg tcgcgctgcc ccacagcgcc
     1141 gcacaaaaaa gcgccagcac ggccaccacc cacgggttac tcagtatttt gcttttactc
     1201 attgtatttc tccaaaaatc gtatttcacg cgcctgttcc cggcggcctc ccgccgcccg
     1261 cgccgcaggc gcgggcggtg ctcttccggc ctccgccccg gcctgccggg cccttacttc
     1321 ttgattttgt cccagtaagc cttcgcggcc tgctcgtttt tgttttgtcc gtattcgtag
     1381 tagcgcacgc ccgccagcgc gtccggcagg tactgctgct tcacccaatg gcccggataa
     1441 tcgtgcgggt acagataatt ctgccccttt tgcgccgcgt cctcgccgtc gaaatgcttg
     1501 ttctgcagcc ggcggggcac cgggccggtt ttgccccggc gcaggtcctg ttccgcggcc
     1561 agaatggcgt tgtgcgccga attgcttttg ggcgcggttg ccatcagcac ggccgcgtcc
     1621 gccagcggca ggcgcgcttc cggcaggccc agctgcaggg cgctgtccac gcaggccttc
     1681 acgatgggaa tggcctgcgg gtacgcaagg cccacgtcct ccgccgcgat gaccagcagc
     1741 ctccggcagg ccgacagcag atccccggct tccagaatgc gcgccagata atgcacggcg
     1801 gcgtccgggt ccgagcctcg aatgcttttc tgcagcgccg agatgcagtc gtagtgcatg
     1861 tcgtcgcttt tgtcgtagcg ttggctggag cgctgcgcca cctggcgcac ggcctctgtt
     1921 gtcagccgct ttgcgccggc cgccacggca aactccaggc tgcccaacgc cttgcgcatg
     1981 tcgccgccgc aggccgccgc cagcgcgtcc agcgcctccg gctgcgcttc aatgcgcacg
     2041 ccgtccagct cctccagctt atgcagagcg ttctccagcc cgcggcgcac gtcctccggc
     2101 gtcaggggct tgaactcaaa caccgttgcg cgggagagca gcgcgttgta aatataaaaa
     2161 tacgggttct cggtggtgga ggcgatcagc gtgaccgttc cgtcctccat gcattccagc
     2221 aggctctgct gctgcttttt gttcagatat tgtatctcgt ccagatacaa aagcaggccg
     2281 ttccggcttt gaaacgtgcc gatctcggcc atgacggcct tgatgtcccc cgtgcccgcg
     2341 ctggtgccgt tcagcttatg caggcgcatg ccgctgtttt cggcaatgat gcgcgccacg
     2401 gtggttttgc ccacgccgga cgggccgtag aagatcatat tgggcacgcg gccgctttcg
     2461 atggccttgc ggaacacgcg ccccgggccg agcagatgct gctggccgca cacctcggca
     2521 agggtgcgcg ggcgcagacg gtcggcaagt ggtgattgca tgagcttctc ctttttgaaa
     2581 cgggcgcgcg cgtcaggata ccaccgcggc gctgcgccag tcgttcagtt tttttgcggc
     2641 gtagccgttg ttcaggaaat aatcgatggc gtacacaagg ctgcggtccc agaaattcca
     2701 ttcgtgcacg ccgttccatt gcaggcggcg gtagccggca agccccagag gcttcaggaa
     2761 tttatgcatg gcgtcgttct gctgcagaat gaaatacggc tccacgtcct gcaggccgtt
     2821 cgtcagcagc acctttggct gctgcgcggc cggcagcgcg gccacccgct gggccagcac
     2881 ccgcaggtcg ctgcttttgg gcagcagcag cccatcgccg aacaccgggc cgaacacctc
     2941 ccgcacgccg ggcgccgcgg ggtccgccag catcatcgaa aggtccaccg caccggaaaa
     3001 gnnnnnnnnn ncctgccccg caaggtaatg caggttgctt gcgggcggca gggcaaggcc
     3061 gtcgccaaaa atgggcgcaa acgtctcgcg cacgccgggc acagacgggt cgcgcagcat
     3121 cagggcaagg tctaacgcgc cggaaaagct ggcgcagccc gcgtacaggt ccggccggga
     3181 aaggcccaga aacagcgcgc cgtagcctcc catggaaagc cccgcgataa aattctgttc
     3241 cctgcgcgcc ggcagcttga acatacggcc cagcagcatg ggcagctctt ccgtgacaaa
     3301 ggtcttatag gcgccgccgt gcatcatgtt ctggtaaaac gagtttccgc agtccggcat
     3361 taccagcacc aaaccgttgt ccgccgcgta gcgcggcgcg gccgtcatct gcatccagtc
     3421 gtctccgctg ttggtatagc cgtgcagcag cgtcagcaca cctttcacgg tatagcccac
     3481 atcagcgggc aggtctgtgg gaaaatacag cttcacgccc acggtggtcc cgaggatctg
     3541 cgatttcagc ttacagtcaa tatacgccat aaaaagcgcc cctttatgta tgataggaat
     3601 cctgcctctt attataaaga aaacccggcc gatttacaat cgtgccgggc aaaagtgcgg
     3661 aaataacggc cccggtgggg ctggtaacct gccgcgcggt ttcgccgcgg gcgaaatgga
     3721 gccggtgggg agcaagcgcc gtggttttgc cgttggggac ccggcgcggc gcagccgtga
     3781 cgggacggca aaagagcggt gcggggtggc gaagccatgg ccgcgaagcg gacaaaccgc
     3841 accgggtcgt ttcgcgcggc gaaacgccgc gcccgatttt gcgcaaaagc cctcaaagcc
     3901 agggcggcca gcgcgctgcc cgcacgggcc tcgttacggc ccggcgggcg ttcaccgcat
     3961 gtttctgcgc ccgcccctgg gcctgccctt ttcctcgtgg cggcagttgc gcacaaaaat
     4021 gcgcaccatc tcctccacgt cctgaagctg ccgctggctc atgcccttca gcaaagggcc
     4081 aatcggctcc tccagcacgc gcgcggcgtc cggcagcagc acataattcg gcgtgatttc
     4141 taaaatatcg cacagcgcga tcagcgtttg aacggaaagt cccgccttcc cccgctcgat
     4201 ctggctgaga aatttttcag tgatatccgc acgggcagac acataatcct gcgtatatcc
     4261 ttttgctttg cgcgctgcct gcagccgcgc gccgatcaat tgcagatcgt attccacaag
     4321 accgcctccc gtaataagag tatcgttaat agctttgtca aataaccatt ttaaaacggg
     4381 tttttcgcgt ttttagattg attatttgaa aatgctttgt tatactgaaa aacagggaaa
     4441 tcgaaaggag acgggcagca agtggatttg tatcagtatt tcgcaaaaga attagactgt
     4501 tttgaatccg atcttcgata cagaagctat atctcctggc ccgccgcctc tgtacggcac
     4561 tgtaaggtgg cagcggacga cgcgcaggcc tggagcgaat ttttaacgta cgttttcggc
     4621 gtcccgctgg atttcagcac cgtggacgcg gccaaacttt atcttttgaa gcaccgatgc
     4681 ctgcccgacg acctttaaat aaaaacaccg ccgccccgga caaatgtccg gggcgctttg
     4741 catcaggtat gggcgtacac ttctgcttct gtgtaaaaaa tgcggaggga ccgtccgcgc
     4801 accggcgccg gcatttcccg ctttggaaag gcccgctgct ttgcctccgc ccgcgggcag
     4861 ccgcccagta ccggccgccc caaccgccgg cagagaaatt cccgttccgc agggccttcg
     4921 cttttccgcg taccgtccgg cggcattttt tgccccggca cgcgacgtaa aagccgctgc
     4981 ttctccccgt ccgccgaatt ttcacggccc cattggaaag acggccgggc aggtgtacca
     5041 gaccacgata tcggacagcg gaatttcttt tgcaggcatc acgcctgaaa gcccgggtac
     5101 agtcgggaac atcacggacg gcagcggatg cgggacggcg tccatcgcgg aggcatgccg
     5161 gcgcgtgcgc gcacccatca agttgacgga atacggcgcg cacgccatcg gatcctcgaa
     5221 aaaggtatgg ggcagctttg caggggagcc cggaagcaag accgtgtcgc cgcgcgggaa
     5281 agacgtccat tcccaatacg gtttacaaca gccgtcgagc catagcagcg gcttcccgcg
     5341 gtgcgggcac aggcacgccg cactgtgccc gggtctgctc cggccggttt gcgggtcgtg
     5401 cagcggcggg ctccgctttt gtttcagggc aggccgccgc aaatcccggc gggatctccg
     5461 ggaaagcggc ggcaaggaag gctcattttt tccgccggcg gctttccgga cacgccttcc
     5521 tggccgccgt ttgcatcggc taccagcgtt tctgcgccgc ctgggccgcc gcctgctgca
     5581 aggcaagaat ctgctgtgca taggcattgt ccagattctg cagcgtatca tacttgtttt
     5641 gcagaatttc ggcgctgccg ttggcaaggg cctgctccaa ttgcatctgg tatgccattt
     5701 tttgctgctc ggccgccgaa agctggctgt tgtagctctg cagcgccgaa gatcgattgc
     5761 tgttcaaggt gtcaagcagc gctgcaaggc tttcgttgcg cccgcggtca atcgtgtttc
     5821 ggttgtttcc gtaggaattg tacatgcccg ccagcgcact ttccgacatg ccccccgtca
     5881 gcccctgcgc cgccagcgcc tggggaaggt cacgcttgct catcatatag ttgatatacg
     5941 cctgctgctg ggcgctgtcg gcgttctggt tcaccccgcg cgcaccgctg tcatagctgg
     6001 cctgcagctg tgccaaagca tcgttgtagt tctgctgaag caaattgctg cggtttgcat
     6061 atgcctcgtt caggtatccc atgtttttgt cataggcggc ctgcgccgcc tcctgttttt
     6121 gccgcgccaa ttcttccgca cgccgtgccg ccgcatcctg tgccgcctgc acctgggcnn
     6181 nnnnnnnntg ctgccctttg cctgccctcc gcctgccccc tgtatgccgt tttccatgcc
     6241 ctgcggcgtg ccctccggct cgccccgggg ctggtatctg tcgtaatgat tcaccgtaaa
     6301 caggccgtat ttcgacgttg ccgtgtgcgt cacctcgccc gtccgtttaa gatgttccag
     6361 cgccgtccgc acctgcttga cggtcagccc cgtctcgtcc gccagctttg ccaggctcgc
     6421 cacgcgctgc ccgcgctcca ccgcaatgcc ctgccaccgc tgcggctcgt agttcaccgt
     6481 cagcagcaga tgaatgaaca gccgtgcggt gttgaggtcg ccgtaccatt cccaccgcag
     6541 aatgctccgg tgaagcaaaa taaaaccgcc ctcaagcatg gcttcctccc tgttcctccc
     6601 gggcaaagcc cgcgtcgatg cattgcccgc agcccagcac atggtcggtg ccgcggccgt
     6661 agtaaacgcg gtcgccgtcg tacagagccg cgccgcattc cgcgcaccgc cccacaggcc
     6721 gcgcctgcgc atacgggtca aacgccgccg aaatccccac ataatgcaat tccatccttt
     6781 tcctcctttt taacaatggg aagcttgctg cgccagccgc cgccgcgaat ctcttcgcgc
     6841 gggcagaatc aacagccttt ttgaacaacg ggaagcctgc tgcgccagcc gccgccgcga
     6901 atctcttcgc gcaggcagaa ccggcagcct tttgaacaac gggaagcctg ctgcgccagc
     6961 cgccgccgcg aatcgcttcg cgcagccg
//
LOCUS       DAWBSB010000113         6944 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_2130_length_6944_cov_3.307882, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000113 DAWBSB010000000
VERSION     DAWBSB010000113.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 6944)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6944)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6944
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2130_length_6944_cov_3.307882"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1419 a         1949 c         2187 g         1389 t
ORIGIN      
        1 tgcctccgcc gtcaagacat acagcccgtc caaaatcagg gaatccgttt aaaccgcgtt
       61 tatcagcaaa gcctgtgcga agaagccggc ggcagccggc ttcttttttg cgcatatatg
      121 ttttaccacc ccaacgaaac aggaggacaa aaacctatgg cacaatttag catccgcccc
      181 gcccggcctg aggactgcgg caccattctg gagctgatcc gcggcattgc ggaatacgaa
      241 aagatgagcg acgaagtcgt tgctaccgaa gagaccatcc ggcatgcgat gttcgacgaa
      301 cacgtcaccc gctgcctgct ggcagagctg gacggcaagg tcattggata cgcgctgtac
      361 ttttacaact attccacgtt tatcggtaca aaaggcctct atctggagga tcttttcctg
      421 tatcccgaat accgtaaggc cggttacggc aagcggctca tccgcgagct gttcaagatc
      481 gccgcggcgg aaaactgcgg ccgcatggag tggtgctgcc tgaactggaa cacccccagc
      541 caggagttct ataagagcct tggcgcaaag ccgatggatg aatggacaac atggaggctg
      601 acgaaagagg accttgcccg tctggcggcg gagtaaggcc gttcgtccaa agcgtaaagc
      661 aaagcggccc gcaccggtac accccggcgc gggccgctgc atttcagctg ttatggacgt
      721 acatcgttgg ggtcgtaggg cggctctggc ataatgagcg cggctacgat ataggccagc
      781 aggcccgagc cccagccgaa gcacagcagc acgaacgcca gccgtacgat ggttgggtct
      841 acgccgaaat attccgcgat gccgccgcac acgccgcata ccatacggtg gccgcgggct
      901 ttatacaaac gtttctggtc catcagtcat ttcctcctgt tgtcaattga atgtgatcgt
      961 tacttcgccc atgccgcatt cgatgtcata aaatgtggcc gcgctgcggt ttacttccag
     1021 ctcgacgccc atgccggaat gagaatagtc gtcgatggtc acggaaccca tgccgctctc
     1081 cagcgcgtag ccgtagtccg ccggccggga gacggccacc tcggcgacgc ccatgccgca
     1141 ggatacctcg tttttgccgt ccaggctgcc gccgtcgatg gtcagcttac ccatgccgac
     1201 atccaggctg ctctcctgtg tgcaggtgag attttttacg gtcatctcgc ccgcgcccac
     1261 ctcaaggtcg gctgtgcggc aggccaagcc gtccgccttc agcgtgcccg cgccgatgga
     1321 aagctccacc tcgtcaaaca cggtgtcccg gggaaccgtg atgaaaaatt ttacgttttc
     1381 ccagttgcgc agctgccagt cgccggtcgt cttaatcgtc cacaccccgc ctgagaccct
     1441 ggactcataa cgcgggctgc cggagacctg caggtcatac gcgtcacccg gctgaatgac
     1501 gacctctgcc gtggagatat cgatttccag ttcccgcacg ctgccggtgg ggacggagcc
     1561 ggagctggag ggggagggcg caccaggggc ggagggagcg ccggaggcgt ctgcccggaa
     1621 catgccgaat tcaccgtcgg taaaagcgtc ggtgtaatag acacgggggc cgttgtccca
     1681 gccgacgcgg atactggtaa gccgtccgcc caggcacagg cccacgatag agagtatgat
     1741 gcccaataca aggcagccgc ccgcaatgct cagcagtgtt tttatgaatt ttgtcattgc
     1801 cgtcttacct cactttccca aacagggtgc ggcacagcga accgacggca cggaagagcg
     1861 ccggaccggc cttggccacg acccataccg tacccgcgcc cagcagcgcg ccgatggaga
     1921 tgcacagcag gctgagaccc atcgtcagga taccactggg aacggatgtg aacagcagcc
     1981 caacgccgcc gccgaaagcc gccacgcccg cgatcattgt ggcaaagccc gcgaacgcaa
     2041 tgccgcacag cccgccgacg atgcccacta cgccgccgaa caggccgccg atgaggccga
     2101 tccagatggg acaggtgaga atgagcagga tgacccagag cgtgcggttg gaggcggagc
     2161 gtccgccgtt ttcgcgttgc gcgccgtctt ggggatagct gtatgcatag gcagggccgt
     2221 gctgcgcgcc gtccgccgcc tgcgcattcg ggaaaccgtc cggagcttcc tcgtccgcgc
     2281 gctggccttc ggcccaagag cgttctgttt cctgcgccgg gacctgggac gcctgccagt
     2341 ccgggccgtc cagtgtaagc tccggaacgg gcgcctgttc cggcttttcc ggagcggtgc
     2401 ttttttccgc tgcatcacgc acggcgggta cgccgaagcc gcagtttgcg cgaatgatgc
     2461 aggccacctt ttgggggctg cccagctcgg ccagcacagc ggcctcgttc tccgggcctg
     2521 cttcatccag atattcgttg tagaatttca gcgcttcgtc gcgctcctct gtggtgatat
     2581 cgcgcagctg ggcttccagg gccgccataa actccgctct attcattact gattcctccg
     2641 aacaatacgt tgtcgatctt cgctttatac tccgtccact cacggcggta catttccaac
     2701 tgctcgcacc ccttttccgt gatgctgtaa tagcggcggt tacggcctgc gaattcacgg
     2761 tcgtaggtct ccaggcactc gctcttctgc agacgccgca gcaccgggta gagggtgctc
     2821 tcggatacct ccatcacact gcgcacatcc tgcgtgatct tgtagccgta tgtgctttca
     2881 ctggccacga cgctcagcac cagggcatcc atcagggctg ctcctgtgtt aaaagccata
     2941 tcgtctttca ctccgtttct gttgcgtggg cggccggggt tgcacggccc cggccgcccg
     3001 gcattccgga tgatttattc cgttgacaat attatattgt gtataatatt atttgtcaac
     3061 acattattga aaataaataa ttattattgg gtgaattgtt tgaaaagcgg acgtgcgttt
     3121 tggctgcatg ataaaaaaca ccggtctttg cgaccggtgt ttttttgaat gtatagaagg
     3181 gcgccgcgcc atgcggcaaa ttcagatttt ttcgctgctg cggtgcgtcg ggctttgccc
     3241 ggtggaactg cgtacgatga gctgcggcgt gtattcgatt ttattcacga agggagtggg
     3301 gatgcgcgaa ggcgtgctct gtgtgatgac catatccacc gccgcctggc agcgggtgcg
     3361 aagatgatgg tcaatggtgg tgaggcccgg cgtggtgatg ctggaagaaa aaatgttgtc
     3421 gaagccacac accgaaaaat cacgcggtac gcgcagacct tgggctgcaa gctcgctcag
     3481 aatgccgagc gccgtcatat cattcacgcc gatgagggct gtggccatgc tctcacgccg
     3541 gatcagctcc gccgtgagct gccgcccaac cgaatattcg tagggcaggc cgtcatcctg
     3601 gctgtctgcc tcggcctggc ggtcggccac cagcacctcc agtccatccg tcacaccgtg
     3661 cagctccaac tgcttgcgga ttccctccag gcgctgctca cgcgccagtg tcagttggtt
     3721 gaacggcgtg gagatgaaca ccagcttgcg gtggcccagc tgatacaaat gctcggccag
     3781 catggcgcct gcgttcacgt tgctcagctc gatggagcag atggacaggt cgtccagctt
     3841 ttccccgatg atgaccgtgg gtgtggacga ggagatctgc tccaccaggc gcgggaagct
     3901 gggcagaaaa gtatagatga taccgtccac atgcgcgccg acaaaagtgt cgagatagaa
     3961 cttttccaga tccggcttgc ggaaggtgtt gcagacgatg acgcggtaat ccagcgtgtc
     4021 ggcatagtcc tcgatggcct ggatcagctc ggcatagtac tggttggtga gcgtgggcgt
     4081 cagaacgaga agcatgttgc tggccttttt ttcgcgctgt tttttgcgct gaggcaggcg
     4141 gtagttcagc ttttgagctg ctgcgagcac gcgctccttg gtctcgttgc tgaaggtgat
     4201 cttatcgctg ttgtttaaaa taagagacac ggtcgcctgc gaaacgcccg caagttccgc
     4261 aacatcctgc gaggtggcct ttttcatggt ccagacgctc ctttttaata atatcttact
     4321 aatattatag cagaaaaatt cttcaagtca aacatagtga ccaaaagcag ggaaaatctt
     4381 ttggcgcttt gagggcttga tatctcttga catcgggctg caggtcggat atactgaaag
     4441 tgcaaaatat tatttatatt gaaatattat tactaatatt ttacacaggc aaaagaaagc
     4501 ggtgcagtag gaatggaaaa gggcggaaac agcggaaata taggagcgcg caagccgatc
     4561 ctggctttgg gggagctgct tgtggacctt gtacccgggc gtgagggcat gcgcatcgag
     4621 gacgcgggcc ctgttgtaaa aacggccagc ggctcggcgg gcattttcgc ctgcgccgcc
     4681 acgctgctgg gcggacgggg cggcttcatc ggcaaagtgg ggcgcgacag cctcagccgg
     4741 atggtagcag gcacactgcg cggccagggg gtggatctct cccgtctcat cgtctcggac
     4801 gaggggcaga tgggccttgc gtttttggaa tatctgccgg aagggcggaa ttatcagtat
     4861 taccgtaaaa attcagtggg cagcctgctg cgggccagtg agctggacgc ggagtacatc
     4921 agcggcgcct atgcggtgca tttccccggc atgttgctgg agctgacgga agaaatgcgc
     4981 ggtgcatgtg aacgtctggt ggagatcgcg cgggagaagg ggattctggt ttcgttcgac
     5041 ccaaacatcc gccgcgagct ttcagcggat gcggccgcac gggcgcgcct gctgtgggcg
     5101 gtgcgccatg cggacgtagt ggcgcccaca ttgagcgagg ggcagatgat tacgggtgaa
     5161 acgagtatcg gcgacgtgct gcgcgcgctg catgcaatgg ggccgcgggt cgtggcgctc
     5221 acgcgtgaca aagacggcgc cgtgctgagc cgcggcggtg aggtggctgt tgcggcgggc
     5281 atcgacgaag agccggtcga ccccacgggc gcgggcgata cgcttgccgc ggcactgtgc
     5341 gtaggcctgc aggaggatat gcccctggca cggctggcgg cgttctgcaa ctgtgcgggc
     5401 acgctggtca tcaagaaaaa aggcgccatc gggatggcgc tgcccgaacg cggacaggtg
     5461 gacgcgatgg tggcctccgg cgtgtgccgt gtggaatgca tgccgctgga aagcctggta
     5521 tgacggcaag gaaatctgag cccgccgcgt accggcgaaa ggcataccgc gcccgcggcg
     5581 ggggcggcgg gcgcggtatg ccggtgcgct gaacggaggc cccgcatagg agagaacgcg
     5641 cgccgcacgg cgcgctgctg aaaaaaggag aagagagaca tggcattgtt aaaagaacgc
     5701 ctgaaaaacg gcgagcaggt actggggacc atggtgacga catttgcaag cccggatatt
     5761 gcgaaaatac tgcagagctg tgggtttgac ttcttcatcg tggactgcga gcacggcgcg
     5821 ttcaccacgc gcgaggcggc gaacatcatt gcggtggcgc gcggcatcgg tatgcccgcg
     5881 ttggtgcgta tcccggagat gcgccgggag cacgcgctca aattcatgga gatgggggcg
     5941 tccgggctgc ttctgcccaa tacggaaaca gccgaacagg cgcgtatgct ggtggactgc
     6001 accaaatacg cgcccatggg acaccggggc gtttcgcttt cgcgtccgca tacggatttt
     6061 aaaagagtag acggacggga atatatgcgc cgggccaatg aagaaaccat cctgatgtgc
     6121 caaatcgaat cccgcaaggg cgtggagaac atcggcgaga tcatggcggt ggaaggtatc
     6181 gacgtgggct tcatcggccc gaacgatatg acgcaggatt acggtatcct gaaccagttc
     6241 ggccatcctg atatcgtggc ggcgtttgaa aaggtcatcg cggcggccga ggaaaacgga
     6301 aagtgggccg gcgtacattt cggcgcggcg gagccgctga aaccgtggct tgcccgcggg
     6361 atgcagatga atatgtggaa cagcgacaat gggcttttgg ccctgggcgc ctcggaaata
     6421 gccgggctgc gcgggaagga gcaggcatga aaatcgtaat tctggacgga tacacggaaa
     6481 accccggaga tttgagctgg aacggctttg aggcgctggg agaggtaacg gtatacgacc
     6541 gcacaccctt tgccgacggc agcggcgaga tcgtgcggcg ggcgaagggg gcggatatcc
     6601 tcatcgtgaa caaaacgccg ctttccgctg aaacgctggc ggaacttgcg ccggaactgc
     6661 ggtatatcgg cgtactggcc acaggctaca atgtggtgga taccgccgcg gccaaagcgc
     6721 agggcgtgcc cgtgtgcaat atcccaacct acggcactgc ggcggtggct cagtttgtga
     6781 tggcgttgct gctggagctg tgccaccatg tgggggacca cagccgctcg gtaaaagagg
     6841 gcggctggag cagctgtccg gacttttgct actggaatac gcctctcatc gagctggcgg
     6901 gcaagacgtt cggcgttatc ggctacgggc gcatcggcag ggcg
//
LOCUS       DAWBSB010000114         6933 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_2132_length_6933_cov_3.310555, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000114 DAWBSB010000000
VERSION     DAWBSB010000114.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 6933)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6933)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6933
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2132_length_6933_cov_3.310555"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    743..752
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1422 a         2212 c         2029 g         1260 t
ORIGIN      
        1 cggcggcgat tgcaagagct gtctttcggt tcagcaaaat gaccaaaagt cattgttttc
       61 attttgtgac tattggtcat taattggagt gactaacggt cataacaaga ctatactatt
      121 caacgttgac tgttggtcag tttgatccgc ggcatcggct gccgcaaaaa ttcaaaggga
      181 gcgttgagca atggaacatt ttgcgcgctg ggtggtacgg caccgcaaag cggtgctggc
      241 ggccgccgtg ctgctgctga tcccatccgt gttcggtgcc gcaggcacct atatcaatta
      301 cgatatcctc acctatctgc cgccggagtt ggattccatg gtgggcgaag gatatctgga
      361 ggacgatttc aacatggcct ccacctccat gatcaccgtg gagaatatgt ccaccgcgga
      421 cacacttgcc ctcaagagtg agctggaggc tgttgcgggt gtaaaaagcg tgctgtggac
      481 gagcgacatc ctggacgtaa ctacgccaaa ggaaatgctg ccggccgaca tccagaaatt
      541 cttttacagc gaaaacggcg ccacactgct gctggtccag ttcgaaaacc caagcgccca
      601 tgccgtgacc atgcaggcgc agcgcaccat caaggatatc ctgcgcaagg actgcttcat
      661 cggcggaatg tccgccattc tggaggacac aaaatccctg gtaaaccagg aaatgcccgt
      721 ctacatcctg tgtgccgtgg ggnnnnnnnn nncgtggggg agtgtctggt gatgctgttc
      781 ctcagcatga aagaaacggt tgtacccctc atcttcatgc tgggcatggt attccccatt
      841 gtctacaact ttggcaccaa tatctttctg gggcagatca gctacatcac cgaggctttg
      901 gccacggtgc tccagctggg cgtgacgatg gatttctcca tctttctgct gcaccggttc
      961 gaggaagaaa agcgttcctg cccggatgag gacgaggcaa tggtgcgcgc cgtgcaaaag
     1021 acgttctcct ccattctggc aagttcgctc accaccatcg caggcttcct cgcaatgtgc
     1081 accatgcgcc tgacactggg gcgcgacatc ggcatcgtaa tggccaaggg cgtgttgctg
     1141 ggcgttgtca gcactgtgac gatcctgccc gcgctcatcc ttacgttccg gcgctccatc
     1201 gcgcggcata cccaccgtac cttcatccct aagctgcgcc gtacctcccg tttcgtggtg
     1261 cgccggtata ttcccatcct cgcagtgttc gtgctctgct tcattccatt cagcgtggcg
     1321 caggcgaaaa cgcctgtata ctacacacta ttcgacagcc tgccgcagga cctcgcgggg
     1381 attcagggta ccagccgcct gaaagaggac ttcggcatga cgacttccca tttcatcatc
     1441 gtggacgacg gcttgccggc ccgcgacatg gaggcgctca ccgatgagat cgagcaggtg
     1501 gacggcatcc gccaggtgct cagctatgaa aagtttttgg gcggcggcgt tccccgggag
     1561 gttctgccca ccgaggtgcg tcagatcttc tgcgcaggcg gccacgagat gctgctggcc
     1621 aattccagct atgagagcgg taccgaagct caaaacgccc aactggacgc tctgaacgcc
     1681 attgtcaagc gttacgaccc caacggcgtg attaccggag agggtgcaat gacaaaagac
     1741 ctgatcgaag tggcggacgt ggatttccgc agcgtcagca tcgccagtat cgcagcggtt
     1801 ttcctcatca tcgccatttc attccgctca gcgtccattc cggtgcttct ggtggccgcc
     1861 atcgaaagtg ccattatgat caatatgggc atcccctatt tcaccggcac gacgctgccc
     1921 tttgtggcca gcatcgtcat cggcaccatc cagctcggcg caacggtgga ctacgccatc
     1981 ctgatgacca cccgcttccg ggaagagctg aatgcggggc gctccaccaa ggacgccgtg
     2041 cagacggccg tggagcagtg cagccagagc atcctcacca gcggactgac gtttttcgcg
     2101 gcaaccttca gcgtttcact ggcttcccgc atggaactta tcagcagcct gtgccgtctc
     2161 atcagccgcg gcgcgctcat cagcatgctt gtgattctgc tggtgctgcc cgcgctgctt
     2221 atcctgacgg cgcctctcat ccggaaaacc acctaccatt ggctcagcaa agaaaaggag
     2281 acgaaaaccg tatgaaaaag ttattgcaga aaaccacggc cgttctggta agcgcggcac
     2341 tggccgtcac ctgcgccctg cccgcctttg ccgaacagcc cacagagggc gtgacgaaag
     2401 acgaaaatgt gtttctgatc ctgaacccgg acggttcggt gagcgaacag atcgtcagcg
     2461 actggctgca cagcgatacc ggctttgacg ctgcggcgga ccgcagcaca ctctccggca
     2521 tcaccaacct gaaaagcgac gtgctgcccg cgcaggacgg cgaaaacctg acctggacca
     2581 ccgaggacaa cgatatttat tatcagggca ccacaacgca gacgcctccc gtgaccgtgg
     2641 atattaccta tacgctggac ggccggtcca tcaccgccga tgcgctggcg ggcaaaagcg
     2701 gacaccttgt gatgcgcttg gctctgacca acaacgaggg acaggaaatg gagattgccg
     2761 gcagccggcg caaggtgtac acgccgtttt tcactgtggc cgcggccacg ctgcccacgg
     2821 gctcctatac caacattgcc gcacagcacg gcaccgtgca gacggacgca aaaacgcagc
     2881 tcgtctgctt tctcgccatg ccgggcatgg ccgagacctt cggcggtctg ctgccggaag
     2941 cgctgtccgg tcttgaagat tacatgctgg acgaactggt catcgaggcg gatgtaaaag
     3001 actgtacacc tccggcattt atgctggccg cagccacaag catggaccag ttgacctcag
     3061 agctggacat tcccgacctc tccggccaga tgagccagct cgaggatgcg accgcgcagc
     3121 tccagagcgg cgcagccaca ctgcatgagg ctacgggcac gctgcagagc aaaatggacg
     3181 aattcgccgc cagctacgcc accttcgaca ccggcctgga caatgctctc agcggcgcgc
     3241 agcaggtgca gagcggcgcg cagaacctgc tggacggtgc aaaaagcctg gacaacggca
     3301 cacagcagct ggcggatggc acacagcagc tgaacaacgg cgcacagcag ctggccggcc
     3361 ggctgaacgg cactctcgta cccgctctgg agggtgccgc cgcgcagcag gcagccctgc
     3421 agcagcagat ggacgcgttg gcagaccagc tcggccatgt gcagctgccc gatgtggacg
     3481 cgctgaaaaa gcagctcggc gcaggtgtgg gccaggtgtt tgacggcgct gcacagggtg
     3541 cggcgaaagc cgccgcccaa agcgtgggcg acgctgtaaa gagcggcgca ctgcaggctg
     3601 cggacgcagc cgttgcgcag gcagcaccgg ccgcggcgca gggcgttgca tccacagccg
     3661 cgcaggatgt ttcctccgcg ctggcagccg cgctgcagcc ctatgtggac agcgggaaaa
     3721 tgacatctga cgaaatgaac gccgtcattg cggaggccgc cggagcggct cagggcgcgg
     3781 ccgccaaggt ggatgcggca ggcatggttc agggcgcggt ggacaaagcc gttaccggca
     3841 atgtgaccgt ggatgccgac gcagcggccg gacagatcgt ggccgcgatg gctcccgaaa
     3901 aggaggccgc cgtaaaccag gtgaccgccg cactgaacgg gctggacacc gcagcgctga
     3961 aggccatgct gctgcagttc caacagcttt ccggcgacgc gcaaacgatg atgggttcgg
     4021 taaaatccct gacggacgca ctgtacaacg ccgacgctcc gtcggaccag aacactgttg
     4081 tgggagcggc cggagctctg gcggccggcg cgcagagcgc gcaggacggc gcggcctccc
     4141 ttcgcgacgg cgcggcgcag ctcaccgccg gcgcggcaca gctgagcaac ggcgcctcca
     4201 gcctgagcgc cggcctgacg cagctctccg gcgcaagcaa aacagtccgt gacgccatcg
     4261 gccaattccg caccggcgcg gggagcctga acgacggcgc ggcacagctg caggacgggc
     4321 tggacaccta tgccagccag gccatcggaa agctgaccga gcttgcgggc tccgccgaca
     4381 cctccgcgct gcaggacgtg ctgaaggcgc tggaaacccg cgcgcagagt tacacctctt
     4441 acacgggcag tgcggacggc gtgcgtgcca atgtaaaatt cgtgatgaaa acagccggtc
     4501 tgccggagga ggaaacggca tcctcttccg acacggaaga ttcggaggcc gcaccgcagg
     4561 acaaaacccc ggacacctcc ttctgggacc gggtcaagaa tcttttctcc tgacatgcac
     4621 ggaaaaagcg cgtggaacat tccacgcgct ttttccgtgt gccgctttcc ggcggagcag
     4681 gctcagcgct ttttcgcaag gaagcgccac aggcgcgtat accaggccgg attgtttttc
     4741 cgccaggcct tgcggtacgc cctgtccgcc tctcgggtgg acatcgggcg caccacgccg
     4801 ggtgtcgtgc ccgccagttc cgtatttttg ccataccgga catgggccat tggttcgcgg
     4861 aaagacattg cgctcctcct tttcttttct atactatacg tattataaca tttttcagat
     4921 cttgcttcaa cgacacgatg cagcggccac agcagagccg catccgctac ggttctgcat
     4981 ggttgaacgc cctcaagccc atcgcgcacg gaatgcgcgt ggcagcggag ggccacccta
     5041 tccccgcccg cgtgcatacc ggcgaccggc gtgtctggcc aaaggcgcac agctgtgcta
     5101 caatgtcaaa aacaacaggt acggaggcaa ccggatatgg agcttttaaa acagcggatc
     5161 ctgcgggacg ggcaggtgaa agaaggcggc gtactcaagg tggacagctt tctcaaccac
     5221 caaatggatg tgacgctgct gaatgagatc gggcgtgaat tccgccgccg tttcgacgga
     5281 gcggacatca ccaaaatcgt taccatcgag gcgtccggaa tcggtattgc cgccattgcc
     5341 gcgcagcatt tcggcagcgt tcccgtcatc ttcgccaaaa agacgcgttc gcgaaatctg
     5401 gacggtgcgc tgtataccgc caaggtccat tctttcacca aggacattac ctacgatgtg
     5461 cagctatcca aaaaatttct cggcccgcag gacactgtgc tcatcctgga cgatttcctc
     5521 gcccggggac aggcactgct gggcctcatc gacatcgtgc ggcaggcggg cgcgcagtgc
     5581 gctggctgcg gcatcgtcat tgaaaagcag cagcagggcg gcggcgcgct ggtgcgggca
     5641 gcgggagtgc gcctggaatc gctggccgta atcgcctcgc tggaaaacgg cacggtcacc
     5701 tttgcgcagt gaccggcata aaaacagggc ctgtgctcca caatgggggc acaggccctg
     5761 tttgcatgcc gcgcatgaaa aaacgcgcag gctgttcgtc attcgccgca cagccgcagg
     5821 atctgctccc gcgcgggcat ggccgggatg gccccctttg ccgcggtggt caggctgccc
     5881 gctgcgtttg caaacgcgac aatgcgctcc cactgtgcct gcggcaatat ccgcagctct
     5941 tccagcgtct tgcccgcaag gcacgacagc agcgctccca gaaaagcgtc gcccgcgccc
     6001 gtagtgtcca ccgtgtccac ttcgcaggca ggcagcaacc cgcggcccgc cgcagcacgg
     6061 tagtacgcgc cccgcggccc cagcgtcacc agaaccagcg ccgcgccccg ggacgccagc
     6121 gcgtccgctc ccgccgccag ctgcacttcg cccgtcagca gctccatttc ttcctccgat
     6181 actttcacga tatccgccag aggcagcgcg gccagcagcg cttctctcgc ccgctccgcg
     6241 ctatcccaca aaaacggacg gtagtttgga tcgtaactga tcatcgcccc cgctgctttt
     6301 gcctcccgcg ccgcctccag cgtagccgtg cggcacggct cgtccgtcag cgaaacagaa
     6361 ccgtaatgaa agatgcggca gtcccgcagc agcgccctgt ccacgtcggc aggcgcgagc
     6421 atcacgtccg cgcccggctt gcggtaaaac gtgaagcttc ggtcgccctc cggcgtcagc
     6481 tgcacaaagg ccagcgtcgt gggatattcc cgcgtcatac gcagaccgcg cacgtccacg
     6541 ccagcatcct ccatcgtctt tttcaaaaac gcgccgaaag cgtcgtcccc caccttgccg
     6601 ataaatgcgg ttttgccgcc cagctttgcg gccattgcca gcacattggc aggcgcgccg
     6661 cccgggttgc gcgcaaacag cgccatcccc tggctgtttt ccccgctggg tgtaaagtcg
     6721 atcaggctct cgcccagcgc cacgatatca aacatacttt cctcctcaaa gtttttggct
     6781 gaattccacc ttttccccat tggggccgag gatggtgaaa aagcgcacgc cccgcgccca
     6841 aaaaggaaga aaccgcaccg cgtcgtccag cagctcgtat ccaccatccc gcagcagcac
     6901 gaacagcgta tctacgtccg cgacgtccag cgc
//
LOCUS       DAWBSB010000115         6847 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_2161_length_6847_cov_3.192432, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000115 DAWBSB010000000
VERSION     DAWBSB010000115.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 6847)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6847)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6847
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2161_length_6847_cov_3.192432"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2112..2121
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1385 a         2048 c         2061 g         1343 t
ORIGIN      
        1 accttggcgg ccgcctccac ctgcgccgca tcggcggtgt cgtaggcgtc cagcaggccg
       61 ttgtatgcgt cgatgggaaa ttttgcgtcg atgggcagat acacacagga ggtgccgtcg
      121 cccggcagcc tcacggcgta ttccacgcgc tcgctgctgc ccttcacggt agccacgttc
      181 tctgcgtact gctccggcgc aaggatctgc tccagaatcg cgcccagctg gatctctccc
      241 aaaatgccgc gcgttttcac attgctcaaa acctttttca ggtcgcccac gcctgttgcc
      301 agcgtctgca tctcgcccaa gcccttgtat acgctctcca actgctcgct cacacggctg
      361 aaggactgct gcagcttttc gtccagcgtt ttctgcagct tctcgtccac agtgtggcgc
      421 atctcgtcca ggcgcttgcc gttgtccgcc tgcatggccg caatgcgcgt ttccatcgtg
      481 gtgcggatgt tctccagctt ctgctcgctc tccagcgcgc ccgttttcat gcgctgctca
      541 atgaactgca gctggtccgt cacggctttt tgcagcgcag tctggcgcac ggcaagctgt
      601 tcgctcagct cccgcaggcg ggtgttctgc gcctccgcca cctgcctttg tgcgtttgcc
      661 tgaagctccg ccgtggactg cagcgttccg tggatggtgc cgcccacctc cgtgcgcagc
      721 gcgcgcatct cttcctgcag ctcctgtgaa aggttctgca ggtcgcgcag tgtcgccccc
      781 tgtttcggcc tgataagtac gcagaccagc gacaaaacag ccgcgatacc cgccagcgcc
      841 agtgtaatat attcggtcat tcgcttgtct cctttgcctt gcctcccggg ttgtgtgata
      901 tccgcggggg cgctttcata aagtatagta tacgaggaat tcctctataa agcaacaacg
      961 aaggcggcga tggatttgtt tgacaagctg aagcaagtac gcatttcaaa acgggcgcgg
     1021 cgcggcgccg tgctggggct gtgcctgtgc gcggcggcgg ggctgggcat gcacctgctg
     1081 ccgggcggcg cgcctgtagg ctgtacacag gaggaccttg cggacacgcg ggccgtcatg
     1141 gccgtgcagg cttccgtctc cggcagcacg gacgcgcaga gcggcgcggc cgcggacaag
     1201 gtggtctatc tgacattcga cgacggcccc tccgccacta cggaaagcgt gctggacacg
     1261 ctgaaggccg agggcgtgcc cgctactttt tttgtcatgg cggcagacaa caacgaggaa
     1321 tacctgcccc tgctggagcg caccgtccag gaagggcatc tcatcgcact gcacacctgc
     1381 agccacgatt acaaaagcat ctataaaagc ccggacgcct attgggatga tctgcagaag
     1441 ctgcgcgaaa agctgcttcc atatgtgccc gcggaccatg aaatcaaatg gctgcgcttt
     1501 cccggcggca gcaccaacac cgtcagccac cgctacggcg gcagtgatat catgaaaaag
     1561 ctgaaagccg atgcggaaag ccgcggcttc acctatgtcg actggaacgt gtgcgcggag
     1621 gattccctcg gcggccaccc ctcggcctct accatttaca acaacatcat ccgcgaggtc
     1681 ggcgacaaaa acacctgcgt ggtgctgatg cacgacaccc cacgcgttta taaaagaata
     1741 ccataaaacc cgcacaggtg cccctttttc acttttcctg tgcggatttt atttatccta
     1801 ttgacattat cacggtatcg tgatataata tagacaacga aaggggcggg caatatgcca
     1861 gtaataagca gattctatgg tttgattatc aagatgtatt tccagcagaa ggagcacaat
     1921 ccaccacatg tgcacgcgtt atataacggt gaatacatgg gcgcgttcac gattgcagac
     1981 ggcaccttga tggagggcga cctacccacc cgggcgcaaa aaatggttgc tgaatggata
     2041 tcggttcacc gggacgaact tcttgaaatg tggcaaactc aaaacttcaa aaccttgcca
     2101 ccgctcgaat gnnnnnnnnn nacaaaatac ggccggaata tgaaaaaaat gcaaatttta
     2161 acagcaaaaa gcctgaaaat ctgtcgtgag aattgtaaaa aggcacaaga tattgtatac
     2221 tgaatgtgtt tcgtatcttg cagcggaaag ggcgtgcgca catgttcggc tatgttttgc
     2281 ccctgacagc agagctgaag gtgcgcgagt ggcacgccta ccgcgcctat tactgcggcc
     2341 tgtgcaagga gctgaagcgt gaatatggct ttttgtcccg ttttctgctg aattacgacc
     2401 ttgtcctgcc ggctctgctg gcggacggta tggcgggcac gcaggcgcgt atgtgcccgg
     2461 agcgctgcat ggccaacccg gtggaaaaac ggccgatgtg ccaaagcacc gcgggccttg
     2521 ccctggctgc ggacgcgctg gtgctgacgg tgtattacaa gctggcggac gaccttgcgg
     2581 acgaacgttt tttcaagcgt ctgcccgctc tggtcctgtg gccctttttg agccgcatgc
     2641 gcaaaaaggc ggcggtccgc aggcccgcgc tggacgagac gctgcgcgaa cagacggccg
     2701 cccagcaggc actggagcgc cgcgccagca aaagcgccga tgaagcggcg gaccccacgg
     2761 cccgcatgac ggccgcgctg ttccgcctgg ccgcgccggg cagccgtgcg ctggagcgca
     2821 tgggcctgtt tgtggggaag atcatctatt acctggacgc ggccgaggat tacgaaaagg
     2881 acacccggaa cggcgcgtac aatgtgtttg cgctgcaggg cctttccaag gcggaggcag
     2941 tggccgaggc acagcgcctg tgccgcatgt gcgcgggcga ggcggctctg gcctacaacc
     3001 tgctggagcc ggggccctgc aaggcgatcc tggacaacat cgtctttctc ggcctgccgc
     3061 agagcatcgc actggccggg caaaagcgca cgcagcccag gcacggcgca taaagctgtg
     3121 cgcggaacgt gatgcatctc cgctccgtgc ggcggaaaca tacagcctga catccgacaa
     3181 cagaaaggaa ttcaaatgga cgcatacaaa gttctgggcc ttaccgaggg cgcgacagaa
     3241 gaagagatca aaaccgcata ccgggctctg gcgcagaagt acaacccgga caattatgag
     3301 gccggccccc tgcgcgagga ggccgaggcc aaaatgaccg aactgaacga ggctttcgac
     3361 gcgctgatgg gcgccatccg caccggcacg cccgtaaaca gcaggcgcgc tgcaaacgca
     3421 ggcgagccgg agccggacac aggcgcggcg gccaacggac gctaccgagc catccgccag
     3481 atgatcaaca gcggcgcggt ggagcaggcc cttgcggaat tgaacgcgat ccagggcggt
     3541 gccaacgacg cggaatggaa cttccttgtg ggcagcgcat attattataa gggatgggta
     3601 gccgacgcgc tgcgctattt ccagacggcg tgccgccttg ctccgggcaa ccgggaatat
     3661 gaggccgcgc tgcgcaattt gcagaacagc gccggcggcg cgatgcccgg caacccttat
     3721 gggaacaacg gtatggcggg cgctcaggcc gtgggctgct cctgctgcga catgtgcacg
     3781 gccatgatgt gcatggacat gtgctgcggc tgcggccggg gaggctgctg acaccatgca
     3841 gaaacgcagc agtgaaagcc gtaagatcgc gctttccggc gtgttcggct cgctggccgc
     3901 cgtcctgatg ctgatgggcg gcatcctgcc gctggccacc tacgtcgcgc ccgcgcttgc
     3961 gggcattctc atcgtgcccg tggccattga attcggcata aaaacaggct atgttctgta
     4021 cgccgccatc ggcctgctct cgctctttgt cgttcccgat aaggaaatga gcctgatctt
     4081 cgtctttttt cttggctttt atcccctgct taaggccagc atcgagcgca tgcgctcccg
     4141 tgcggcccag tgggccgtca agctggccgt gttcaacgca tgcatcgtcg gcatgtacgg
     4201 catcatttta tttttgtttc ccatcggcgc cgttgtcgag gaattcgaga gcatgggcat
     4261 cccgttcacg gggctgctgc tcttgatggg caacgtcacc tttgtgatat acgacgtcgc
     4321 cgtggcccgc atcatcggcc tgtactgcgc ccgctttcgg gcaaggctga tgaaaatgca
     4381 ctgagccgcg gggcgctggc tttacaacac cgggaggttt gatattccat gcagctatga
     4441 agcgccgggc ccttttgcaa cagcggagcg ggaagcgctg cttccccatg ggcttgccca
     4501 ccgccacgaa gggtgacgag gatgggagat aacccgtttt cggcaatgtg ccgaagcggg
     4561 cggcatcgaa agattcggcg gatgctccca cggccggggt tccgggcggc cgacaccatg
     4621 cgggcaaaac acggcggcaa cgccgaaaca gagacgcatg gcacaccggg attttacaca
     4681 taaaattttg cgtccccgcg cggcgggcgg cgtttttttg cgggcggtgc agtacgccgc
     4741 ccggacagac gttatccgac cgcggcagcc tccgggcgcc gccgcggtac gcctgtctct
     4801 tcggctgtgc cgcagcttaa gcggcgcggc acaaaataca taaatgcaaa gacgcaaaaa
     4861 cgcgggcgcg ccggccgggt acgccccgga cgtgttgtgt ctttcggcag gccatgcggc
     4921 gcttttctgc gcggcggcat ggccttggta ttgttttgtg tttttgcgtc tttcgcattg
     4981 taccattttt taaaaatgac aatgttggga gatagaattt atgtgtggaa ttgttggata
     5041 tatcggctcg cgccagagca cggacgtact gctggacgga ctttccaagc tggaataccg
     5101 gggctatgat tcagccggag tcgcattgtg cgtaaacgat gagatccggg tcgtcaaagc
     5161 acagggcagg ctggccgttc tggcagaaaa actgaacggc atgccgccca tggcctcgca
     5221 ctgcggcatc gggcacacac gctgggctac gcacggcgcc cccagcgacg taaacagcca
     5281 tccccattgc gccgcgcgcg tcagcctggt gcacaacggc atcattgaaa actacgccca
     5341 gctcaaggag caccttatga aacgaggcta tgaattctgc agcgaaacag acaccgaggt
     5401 gctggtaaag ctgctggacc acaactatca cggcgaaccg ctggacgcca tcctgcggac
     5461 gctggagcag gtgcgcggca gctacgggct cgccatcctg ttcaaagatt ttcccgggca
     5521 gatcttctcc gtgcgcaagg aaagccctct gatcgtcggt tacggcgccg gagaaaactt
     5581 tctcgccagc gacattcccg ctctgctgcg ctatacccgc gattatgcgg tgctggaaga
     5641 gggcgagatc gctgtgctga gcgcggataa gatcgacgta tacgacgctt tcggccatcc
     5701 ggtgcaaaag cagcacctca cggccgactg ggacatcggc gctgccgaaa aaggcggtta
     5761 tcctcacttt atcctcaaag agatccacga gcagccggag gccctgcgcg ccaccgtcaa
     5821 tccgcgcgta caggacggcc tgcccgcgtt gggcgtgccc ggactgacgg acgaatacct
     5881 tgcaggagtg gagaatatcc acatcgttgc ctgcggcaca gccatgcacg ccggcatggt
     5941 cggcaagacc gccattgagc gcatcgcccg cgtgcccgtt tatgtagaca tcgccagtga
     6001 attccgctac cgggacccca tccttggcaa aaacgatctg gtggtcatca tcagccagag
     6061 cggcgagact ctggatacgc tggccgcact gaagcttgcc agggagcgcg gcgcaaaaac
     6121 acttgctgtg gtgaacgtgg ttggttcttc catcgcccgc gcggccgact gggttctgta
     6181 cacctacgcc gggccggaga ttgccgtcgc cagcaccaag gcgtacagcg tgcagatggc
     6241 cgtgctgtat ctgttcgcgc tgcgtctggc gctggtccgc ggcgcacagg acgaatgcat
     6301 cgtgcgccac ctgacggtcg agctgcagcg cgttccggag ctgctgcgcc ccattctgaa
     6361 ggactgtgac aacatcaaat acctcgccag ccagttcatg aacagcgatc atctgttctt
     6421 catgggacgc gggttcgact atgcgctctc gctggagggc agcctcaagc tgaaggagat
     6481 cagctatgtg cacagcgagg cctacgcggc cggcgagctg aagcacggca ccatctctct
     6541 aatcgtggag ggcacgcccg tagtggcgct tgccacacag gacagcgtat atgaaaaaac
     6601 catcagcaac gtcaaggagg taaagacacg cggcggccgg gtgatcctgc tttgcaaaca
     6661 ggacgcaaag gtccctgccg acgcagcgga tcatgtggtg cgcctgcccg aatttgaggg
     6721 cgtgttcatg ccgctgctgc tgatcgtacc gctgcagctg tttgcctatt acatgagcgt
     6781 gctgcgcggc tgcgacgtgg ataaaccccg caatctggcc aagagcgtga cggtggaatg
     6841 acccttc
//
LOCUS       DAWBSB010000116         6807 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_2171_length_6807_cov_3.220972, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000116 DAWBSB010000000
VERSION     DAWBSB010000116.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 6807)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6807)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6807
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2171_length_6807_cov_3.220972"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1169..1178
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2340..2349
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3526..3535
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1333 a         2109 c         2114 g         1221 t
ORIGIN      
        1 tatgcgaaaa gcaccgctct ggtagacgac ctgtaagctg ctgctccgta aaaagaggcc
       61 ccggcagcag ccggggcctc tttttgcgct tgctttaaaa gatggaatag gtgaggaaca
      121 aaaccgccgc cagagaactg accagcgaaa cgacggtgat ctgtgtgttg atggacggcc
      181 cggcggtgtc cttgaacgga tcgcccacgg tgtcgccgat gacagcggcc ttgtgtgcgt
      241 cgctgccctt gccgccgaag tggccggatt caatgtactt tttcgcgttg tcccacaggc
      301 cgcccgcatt gctcatcaaa agcgccagca gcaggccgga gatgatgttg cctgtcaggt
      361 acccgccgat ggcctgcacg ccgccgatga agcccaccag cagcgtggcg gcaatggctg
      421 taaggcccgc ggggaccagc tcacggatgg cgcccagcgt ggaaatatcg atgcagcgcg
      481 cataatccgg tttgccggtg ccttccttca ggccggggat ctctttgaac tggcggtgga
      541 tctctttcac catcagctgg gagttgcggt ttacgcccat aatgagcatg gccgagaaca
      601 cggccgggat ggctgcgccc accagcatgc cgaagaacac cagcgggtcc atcaggtcga
      661 acgtgatctc cacgcccgca gcctggacca cctcgcggaa cgcgcccagc agggcgatga
      721 cggtcaggcc cgccgcgccg atggaaaagc ccttggtgat ggcctttgcg gtgttgccgg
      781 ctgcatccag ctcgtcggtg atctccagaa cctcatcgcc aaggtccgcc atttccgcca
      841 cgccccgggc gttgtccacg atggggccgt aggcgtcgct ggagatgatc atgcccacga
      901 tggaaagcat gccgatggcc gcacaggaga tgcccagcat ctcataccct gcgcccaggg
      961 gcgcgcacag cttgtaggaa atgagcgccg caagcccaat gcccaccagc gcgggcagcg
     1021 cggacaaaag cccataggaa aaaccggaga ggatggtgaa cgccgggccg ctctggcagg
     1081 cctcggccac cttggcaacg ggcttcttgt cgtcgcccgt gaagaaatag gaggtgatgc
     1141 cgatgatgac acccgccaga atacccacnn nnnnnnnncc gcaggtgaga taggtgccgc
     1201 cgttcagcgc gcgggaagga ttcccgtttt tgcccaggcg ggcgcacagc acgcccacaa
     1261 cagaggccag caggcccagc gtggtgacgc agaacaggaa gggcacctcg cccagggaaa
     1321 gggcgatgac gatggtggcg gccatggcgg ctacattgga atcgaacagg tcggcgccca
     1381 tgccggccac gtcgcccacg ttgtcgccca cattgtccgc gatcacggcg gggttgcgcg
     1441 ggtcgtcctc ggggatgccc agttccacct tacccgtcag gtcggccgcg atgtcggccg
     1501 ttttggtgaa gatgccgccg cccgctttgg caaacagcgc cagagagctt gcgccgaagc
     1561 tgaacgccat aatgacgtcc gcgtcacggg agatgatata aatgagcgcc gcgcctgcca
     1621 gagaagtgcc gatgacggcc atgcccatca ccgcgccgcc gcggaagccc gccataaaag
     1681 atttctggat ggattcccgc gcggccgtgg ccgccttcag gttggcgatg gtggcgatgg
     1741 agatgcccac gtagcccgcc agcgccgaaa gcacggtgcc gcacaggtag gcaaacacca
     1801 tcagcagatt tttgcccacg cccgcgccgg cccagacagg ctgcggaaag aacaaaaaga
     1861 taacggccat cacgatgatg gagaaaagcc ccagcacacg gtattcccgc ctgaggaagg
     1921 tgaacgcgcc ctcgcggatc agcgcgccga cctcatccag cttcggattg gccgtgggca
     1981 gtttttttac ccagttgtag aaatacgccg cgacaaaaaa ggatacgagc gatacggcaa
     2041 gcgccgcata gacccagaac atgataaata tacccccttg aacgataaag tgtccccggg
     2101 gcggccgcgt tttggggcga gccgtcgccg ggccggatgc tccgttttaa atgcaggggc
     2161 aaggcggcgc atggcctccg cgccgttgag aaagcaggca gggccggtgg ctgcgacggg
     2221 cccggcaggc gtgccggcag tgcacaaggc agcgccacag ggcgccgacg cccacggcgg
     2281 ctgccgcaca cgctgcgcgc tgcggcggca tgccgcgccg cgcgggcgcg ctgaaagcgn
     2341 nnnnnnnnnc agctgtcccg ctcctgtatg cgctcctggg caaggagggg aaggctgtcc
     2401 ggcgtatcgc tcccgtcggc ctgcggcacg gacgggcaaa gcgcgaacag gcagacggcg
     2461 cacagcacaa gccgcaaaaa cagcatgaga aaacgcattt caccccctcc tttcccaaaa
     2521 ctcaaaacaa caaaaacaaa acttttcacc caatataaca tattagtgta aaaggcaaca
     2581 aaaagaaagg ggtggtgact atttttggca gaacacacaa aaacccggca gatgttttta
     2641 cacatctgcc gggcatggcg ttgcaatgga aaaggggaac gggccgcggc gtcattccac
     2701 cctgatattg ggcctgtcct ggtccccgtc ggccttgaac atcgtgaaag cagcgggttc
     2761 gctggtgaag aaataatatt ccgtattttc gcagagcggc ggcgtcagat aattggggat
     2821 aatgttgagg tcgtcctccg tgcgggcaca gaagcccgct tcggccatcg tctgcgcggg
     2881 aatgccccag cagtagcagt cggcgtattc cgcgccgcag tccgccatca gccggctgac
     2941 ggccgcgccc agccgggaga aggcctccgg gacgccggta taatccacga tgcgcagcac
     3001 gcacgtgccg tttacgggga tgcaccgcgt tgccagcagc gcttgtaccg caccgcattc
     3061 ctcaacggcc cacacatcgt accgctggcg cgggtagtgg aaataccgcc gcgccagata
     3121 ccagaggtct ttataaggcc tgcgggcggg gtcgggcgta tagccgcatt tccgcagcgc
     3181 gtcctcgtcc ggcagaagac gcagcacggc gctgcctccc gcgggaagga tatccctgcg
     3241 ggccacgcgg gccacggcgt agctggcctt atcggccaga cggtaatagt ggggcaggcg
     3301 ctctgcggtg taacccagaa aggtgtagaa cgccatggtc ctgggccgga tgttgttgca
     3361 ggccatcacg cgggcgtttg tcaggcccgg cagcgcggcc atcagttcca gtccgctgcc
     3421 gttgtgccct ttgacggcac accagatgga cacccagatg tcgggcgttt ccagcgcgtt
     3481 ggcgcggata tatcccacga tggcaacggg cgcgccgtcc tgctcnnnnn nnnnncggcg
     3541 aaggcgaact ggagcgggcc gtccgggccg aagggccggt agtagaaatc aaaaaaatcg
     3601 ggcagatgca ccagcgggtg cttttcgccc cagtgcgcgt ccagaaatgc agcgacggcc
     3661 gggccctcgt ccggccgtgc aaggcggaaa gtcagcgggg aagtgctcat aaaaacgcgc
     3721 tccctcccgt gacgggcagt gttacgcccg tgataaagcc cgcttcctcg gaaagaagga
     3781 acgccatggc gggcaccacg tcctccacac gggcgttgcg cttcatgggg ttggcttccg
     3841 cgctggcctg tacgatcagc tcgctggtgt cggccaggaa tttcgtttcc atcatgctgg
     3901 gcatcacgca gttgacggtg acgccgaagg aggcgtagtc cgccgcaagg cttttggcaa
     3961 ggccctgcag cgtgcttttc accattacat aggccgccgt atttttgggc ggcgcgccca
     4021 gcagatagct tgtcagcatg aacaatacgc gcccctgccg cgccttcgcc atggcgggca
     4081 ggaacgtttt gcacagcttc accgcgctgc ggatctgcac gctcatgtcc cgctcaaagc
     4141 ggtcctcgtc aaaattcttg aacctggtat ttacgacgcg cagcgcgggc aggtgcacga
     4201 gatgcgtggg cgtggggtaa cgcgccgcca catcccggac gaacagctcc agcgcggccg
     4261 ggtccgtcag atccacatcg taggtcttga tggcgccggg gtatttccgg cacagcgggg
     4321 ccaggtggga aaggtcgccc gcgccctggg caatgaagac gtcgccctcc cggtacagac
     4381 ggtcgatgag cgcggtgccc acgtcgctgg acgcgccggt gatgagataa acagacatgg
     4441 cagaacctcc gttttgtgtt tcggtatggg agcgcacggt ttttcacgcg cggcggtcaa
     4501 cacgcgttgc ccgccgcacc ccatgcgccc ggaaaaacaa ggcaaaacgc ggggcgcggc
     4561 tcagacggcc acgcccgcgc ggatgacgcc gcgcgtcacc ttctgcccgt tctggttggt
     4621 gatgacggcc ttgacggtga tctcgcggaa cgtctcgttc acctccgtca cagtgccgct
     4681 gacggtgagc acatccccaa tgaacacggg ccttgcgaat ttgcattcca cgccgtggag
     4741 caggcagtgc tcaccgggca gatatacgcc cgccagcgtg gaaaacaggc tcgcgcccag
     4801 catgccgtat accacgcggc ccggatagcc gcgcctttgc gcataggcct cgtccatgtg
     4861 gatggggctg cagtcgcctg tcagttcgta aaacagcgcc attttttcag ccgtaacctc
     4921 cagggtaaag tccgccgtca ggccgggcga aatgtctgcg agcgtatagc tgttcataga
     4981 aatattcctc cgtctgtccg gcgcgccgtg ggccgggatg taaaaaacgg gacgggcgca
     5041 gtgcccgccc cgggaaacgg tacaaaaggg gaaaaagccg cgcgcatcag ccccgcaggc
     5101 ggatgatcag atccaccatg tcgcccacat tggcaagccc gcgcacatcg tccagcttga
     5161 acttgacgcc gaattgcttt tccatggccg tcagcaggtt gatctgctcc aggctgtccc
     5221 agtcctcgat atcgtccgcg gtggtctccc gcgtaagcac aagggaatcg tcgtcaaaat
     5281 tgtcgcggaa gatattctgt accgcgtcaa agatctgctg ttcattcatg cgtgtctgct
     5341 ccttattata aaagtacggt ctccatcacc gtgttcagcg gtgtacgttc ggcgggcacc
     5401 gtatagcgga ataccgagcc cgtttcgccg ccgcttattt tttcaaagcc aattgtagca
     5461 taaaaatccg ccaccaacaa gttctttgcc gtgggaagat agatgccgtt gatggttttt
     5521 atgccgcgct cccggcacac gtccaccaga cggtcgaaca gggcgtattc cagattgcgc
     5581 ttgaaggttc ggcagctcat gatccaaagc tcgatgtcca gcgcgtcgcc cgtctggcgg
     5641 ccgatgagcg ccgtcacgat gccgttgtcg ccgaacttgt ccagcagccg tccgtaaagc
     5701 gtcacgcagg ccgggtcgtt catcagcgcc tccacctcgg cggcggtgta gcggcgcgtg
     5761 gtgagattga actggttggt tttgttgatg agctgtgtga tgcgctcggc gtgaggcgtg
     5821 tcgaaggggc ccagctcgca gcgcatctca aggctgcgca gatagtcttc gtaattgccg
     5881 aagctctgcc gggccgcggc gcgctgcagg ttctgccggt acatctcgcc gcgccgccgg
     5941 tcgtcggcgg agacggccgt ggcttcaaac catccgcccc ggtccagcac gcgggcatat
     6001 tcctcggggg ctgccagctc gggcaccgtt acggtgggca gctcctgccg cacgatctcc
     6061 cgctccgccg ggttatcatc caaaaatacc aggctgtcgg gcaggatatt gatctccccg
     6121 gcaatggcgt cgatgttgcg ggactttggc tcccagttgg ccttgaagca caggaagtcg
     6181 tccgctttga gcacgctgtc cgcccgttca aagccggcct ttgccgcggc ttcctcattt
     6241 ttgctgcaca cattcaaaag cacgcccatg ccggaaagct cttttaagta gctctggaac
     6301 gcggtgtagg ccatgcctgt gggcgtctca ttgcccagct cgacgccctc ggggccgtcg
     6361 tcgccgatga cgccgcccca gagcgtgttg tccaggtcca gcacaacgcc ctttttgttt
     6421 ttgccgaaaa tgcttttgat gatattggcc acgctgtggc acaaaagcgg gatgtactgc
     6481 atatccagcg catatttgta ggcgtaccac gccgcgggcg cgcaccagct gtctacgccc
     6541 acgctggccg agagccagca cagatcatgc agatagaaat tgggcatggc cgcggcccag
     6601 tccgccagca tggcgttcat gcggttgaca aagcgcaccc ggccgtgggg gtccacggcg
     6661 tccatgctgc ccatcacgcg caggccgggc agctcaaaat tgttctggac gaccgggcag
     6721 ccgaagcggg ccgccgcctt ccacatgccc tgccagcggg cgcattccgc ggcgaatttt
     6781 tcttccgcct gttcgggtgt gtccgtc
//
LOCUS       DAWBSB010000117         6754 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_2188_length_6754_cov_3.986267, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000117 DAWBSB010000000
VERSION     DAWBSB010000117.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 6754)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6754)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6754
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2188_length_6754_cov_3.986267"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1473 a         2025 c         2146 g         1110 t
ORIGIN      
        1 gagaaattca ttgccgcagc caacagggaa aacctggagg ccaccgtcgt ggccgaggtg
       61 acggcggagc cgcgccttgt catgcgctgg aacggcaata tcatctgcga catcaagcgc
      121 aaattcctga actccaacgg cgcggagaag catatggacg tgcgcgtgcc ggcacgcagc
      181 gtggaaccct gcaaagcgcc ggagggcacg cttgaggaaa aactgaccgc gctgatgagc
      241 gatctgaaca cctgcagcaa aaaaggactg tccgaacggt tcgattccac catcggcgcg
      301 ggtacggtac tgatgccttt cggcggccgg acacagctca cgcccgtgca ggcaatggtg
      361 gcaaagctgc ccgtgcccca cggcgagaca aagacctgct ccggcatggc ctggggttac
      421 aaccctttca tcgccgaaca gagcgagtac cacagcgcct acctggccgt ggccgagagc
      481 gtttccaagc tggtggccac cggtttttcc gccaaaaacg cctatctcac gttccaggaa
      541 tacttcgagc gcctgggcgc ggaccccacc cgctggggcc ggcccatggc cagcctgctg
      601 ggcgcgctgg acgcgcaggt ggaactgggc gtcgctgcca tcggcggcaa ggactccatg
      661 tccggctcgt ttgaaaacat cgacgtgccg cccacgctgg tcagctttgc tgttgccgcg
      721 ggcaatacgg acaatgtcgt cagcccggag ttcaagctgc ccggctcgaa gctggtgctg
      781 ctgagcccgg ccatgcgcga cggcctgctg cccaatgccg ggagcctgaa gctgctgtgg
      841 gcgcaggtgg agacgctcat caaagagaaa aaggtgcttt ctgcctacac gccgtccatg
      901 ggcggtctgg ccgaggcact gttcaaaatg ggcctgggca accgcctggg cgtgcgtttc
      961 gccccgggct ttgacgccgc aaagctgttc ggctacgcct acggcagctt cgtgctggag
     1021 ctggcggagg acatcgacgt gggcacgccc atcggcgaga caacagcgcg ctatgtgtgg
     1081 gaactgaacg gcgagacagc cgacctggcc aaagtgcagg aagcctatga agcaaagctg
     1141 gagccggtgc tgccctaccg tacggcagag gcagccgggc cggcgcagga ggcgcccgcg
     1201 ctgaagtacg aggcgctgaa ccgcgtggcg cccaaggtgg gcacagcaaa gccccgcgtg
     1261 ctgatcccgg tgttcccggg cacaaactgc gagtacgatt ccgcccgcgc catggagcgc
     1321 gccggggccg agcccgaggt attcgtcatc aacaacctca ccccggccca tgtggccgag
     1381 agcgtggctg ctgtgaagga gctcatcggg cgcagccaga tgatcttcct gccgggcggc
     1441 ttctccggcg gcgacgagcc ggagggcagc gccaagttca tcacggcgtt tttccgcaac
     1501 ccggccatca ccgaggccgt gcgcgggctg ctgcaaaagc gcgacggcct gatgctgggc
     1561 atctgcaacg gcttccaggc gctcatcaag ctgggccttg tgcccttcgg cgatatcgtg
     1621 gagacggatg atacctgccc cacgctgaca ttcaacacca tcggccgcca ccagagtatg
     1681 ctggtgcgca cccgtgtggc gtcgaacctt tcgccctggc tgcagtatac aaagccgggc
     1741 gatgtgcaca cggtggccgt cagccatggc gaaggccgct tcgtggccgg agccgaaacg
     1801 ctggaccgcc tagtgaaaaa cgggcagatc gccacccagt atgtagacct gtccggcaag
     1861 cccaccatgg acgtgcgcca caaccccaac ggcagctact atgccgtgga gggcatcacc
     1921 agcccggacg gccgcgtgct gggcaagatg ggccattccg agcgcagcgg cgacaacctg
     1981 tataagaacg tggacggcaa caaataccag aagctgtttg agggcggcgt ggattacttc
     2041 aaactgtaag aggagagcac aatggcacac gatacctata tttccccgtt ccagacgcgt
     2101 tacgcgtcga aagagatgca gtacatcttc tcggaggaca ataaattcaa gacgtggcgc
     2161 aggctgtgga tcgcgctggc gaaggccgag cagaagcagg gcctgcccat caccgacgcg
     2221 cagatcgccg agctggaggc gcacaaggac gacatcaatt acgatgtggc cgccgcccgg
     2281 gaaaaggagt gccgccacga cgtgatgagc cacgtctatg cctacggcgt gcagtgcccg
     2341 gaggcaaagg gcatcatcca tctgggggcc accagctgct atgtgggcga caacaccgat
     2401 atccttgtca tgcgcgaggg gctggaggta gtgcgccgca agctgctgaa cgtgatggcc
     2461 ctgttggcag aattcgcgga cgaatacaag gccatgcccg cgctggccta cacacatctg
     2521 cagcccgcgc agctcaccac cgtgggcaag cgcgccgcgc tgtggctggg cgagctgtat
     2581 atggattacg ccgaaatcga gacgcgcatc aaggggcttg cgctgctggg cagcaagggc
     2641 accacgggca cacaggcaag ctttgtggag ctgtttgagg gcgacgacgc aaaaatcaag
     2701 gcggtggagg ccgacatcgc cgccgcgctg ggttttgaaa aagtggttcc cgtttcgggc
     2761 cagacgtatt cccgcaaagt ggactacaat gtgctgtccg cgctgtccgg cgttgcgcag
     2821 agcgccatga agttcggcaa cgacatgcgc ctgctggcca atttcaagga gatggaggaa
     2881 ccgtttgaga aaaaccagat cggttcgtcc gccatgccct acaaacgcaa tcccatgcgg
     2941 tgcgagcgca tctgcgcgct ggcccgctac gtgatgatcg acgtgctgaa ccccgcgttc
     3001 actgcgggca cacagtggtt tgaacgcacg ctggacgatt ccgccaacaa gcgcgtggcc
     3061 gtggccgagg gctttttggc cgtggacgcc attctgaaca tcatgctcaa cgtgtgcgac
     3121 ggcctggtgg tatacccaaa ggtcatccga agccgcatta tggcggagct gcccttcatg
     3181 gccagcgaaa acatcatgat gagcgccgtc aagaaaggcg gcgaccgcca ggagctgcac
     3241 gagaagatcc gtgtctatgc gcagcaggcg gcaaaggtcg tgaaagagga gggcggcaaa
     3301 aacgacctca tcgagcgcat ctgcgccgac ccctctttcg ggctggatta cgaggagatc
     3361 gaggccatcc tcaagccgga ggacttcacg ggccgcagcg ccgcacaggt gaaggaattc
     3421 ctggcggatt gcatccgccc gcttctggag gaaaaccgcg cgctgctggg agagaaagct
     3481 gaactttctg tataatcaga tgggaaaatt acaaaaaacc gccccggtat gcaacaaagc
     3541 gccggagcgg ttttttatgt gaaaggacaa ggaggcaggc gcgccgttca gatggcgcgg
     3601 aagcccttgc cgatgatctc gctggaattg acgatggtga tgaaagcgcc gggatccgtc
     3661 cggcggatgt aattgcgcag ggagacagct tcgcgccggt tcagcaccgt ggtgatgacc
     3721 tgctcgtccc ggcccgaaaa ggcgccgtgg gccgtatata cggtagcgcc gcggtgcagc
     3781 gtgcccatga tgaactcatt gatcttgtcc ggctggcggg acacgatggt gacctttttg
     3841 cgcacgttga tgccttcgat gacgccgtcc accacaaagg ccttggccag caggcccagc
     3901 acgcagtaca ggcctgtgcg cacgccgtac agcccgccgg caaacagcgc gatgcagaaa
     3961 tccgaaacga gcagcgcctt gccgatctcc agactcgtgt atttggaaag gatcatggca
     4021 atgatgtcgg tgccgccgct ggaggcgccg atgttaaaaa taatagcgct gcccgccgca
     4081 ggcaggataa cggcgtagca cagctccagg aacggcgcgc tccagcaccc agacatagaa
     4141 cgagagcgcc accgaagagt aaagcgtggc cccgatgacc gcgcgcccca gaaacaccag
     4201 ccccagcacg atgagcacgc cgttgatgat aaacatgaac gtgcccacat ccatatgcgg
     4261 cagaagcgtg gccgcgatga tggagatgcc ggacgtaccg cccattgcaa aattattggg
     4321 tgatttgaaa aaatggatgc ctgccgccag cagcacaagg ccgaagttga gcagcaggaa
     4381 ccagatgata tgcgcgcggg taaagatttt tttagcagct tccatcttgg ggtttctccc
     4441 gttctgtggt ataatatgtc cggttggtga atgcgagccg caggcgaagc atgagcctgg
     4501 cggcatagat atcatgtttt ttagtgaaca cgagcggtac gcgaagtgcg agcttagaaa
     4561 tatggtataa taacctcaaa gccgccgctt gcgcggctgc gcgtgtgcat ggcacacgcc
     4621 cctgtgctga cgcacagggc tttgagaaca ttgaaccatg caaaaccgca gcaggtgcgg
     4681 ttttgttcta gccacggctg gcggagacat gatataatat ataataatat ggggtttttt
     4741 agccgtttcc gcaatgtttt gtctctgacc gcccccattt tagcacactg tccgcgtcgg
     4801 tacaagccgc gcggagcctg ccggaaaaat tttttgcccg gtgcgggccg cgcccgcgaa
     4861 aaaatatgtt tttattgccc gggacagaag gtgtgcgcgt cccgcgataa ggaaagattg
     4921 gaacccactg catgaaattc agcgatttga acctgattcc cccaattttg aaagccgccg
     4981 ccgaggcggg ctacgaagaa ccgtctccca ttcagcaaaa agccattccg cccgtactgg
     5041 ccgggcggga cctgctgggc tgcgctcaga ccggaaccgg caagaccgcc gcgttcgcca
     5101 tgcccattct gcagcgtctg gcggctgcgc cagcaccttc gggcgggcgc cccatccgag
     5161 cgctcattct cacgccgacc cgcgagcttg cgctgcagat cgacgaaagc tttgcggcct
     5221 acggccgcca tatgcgcctt gcacactgcg ttattttcgg cggcgtgaac cagaatcccc
     5281 aggtggcccg gctgaaaaag ggcgtagata tcctgacagc cacgcccggc cgcctgaacg
     5341 atctcatcgg acagggctat atcgacctga aaaatgttga gatattcgta ttggacgaag
     5401 ccgaccgtat gctggacatg ggctttgtac atgatgtgaa gcgggtgctc gcctgccttc
     5461 cggggcaaaa gcagacgctg ctgttctcgg ccaccatgcc gcgggagatc gaggagctgg
     5521 ccgacggcct gctccacggc ccggaaaagg tgatggtcgc gccgcccgcc acgacggtgg
     5581 agcgcattga gcaaagcgtt tattttgtgg acaagggcaa caagcgccac ctgctggcgc
     5641 agctgctgca aaaaccggag gtgaccagcg cgctggtgtt cacccgcacc aagcatgggg
     5701 cggaccgcgt tgtgcgggag cttgcccgcg cgggcatcgg agctatggcc atccacggca
     5761 acaaaagcca gaacgcccgg caggacgcgc tgggccgctt caaggacggc cgtatccgcg
     5821 tgctggtggc cacggacatt gccgcccgcg gcattgacgt cagcggcctt tcccatgtgt
     5881 tcaactatga tctgcccaat atccccgaga catatgtgca caggatcggg cgcacggggc
     5941 gcgcggggca cgcgggcgtg gccgtaagct tctgcgactt tgaggaaaag gaatatctgg
     6001 cggatattga aaagctgacg cacatccgca tcccgtccgt ggaacacgag tggccgatgc
     6061 aggtgtttga aaaagaagag aaacagacgc aggggcgtgg ccagcgcgcg ccgcgtgccg
     6121 agcgcgcgcc tgccgtgcgg ccggccgtac aggcgcagca gcaggcccct gcgcggaaaa
     6181 agccggtccg ggccgccgcc cggccggaaa aaagtgagga atttcaaatg gaacagacga
     6241 atacgccaca gcgcccgcag caggacggcg cggctccggc cgaaagtgca aagcgccgcc
     6301 gtcggcgcgg cggccgccgc cataagagcg gcgggcaggg cccggccgcg gccagccagg
     6361 cgccgcagaa aaaagagagt gctccgcagg cgcagctcca gcagccccaa aaggagaaac
     6421 cggcaaacgg cgcgccgcgc ggcaagaacg gacgcggcga aaaagccgag cacgcgcccc
     6481 gcgctgaaaa gagcgcaaag tctgcaaaag aggagccgcg cttttccgag gtggacgaca
     6541 gcatccagct tatcgcacgt agggcccctg cacaaaaata tgccagcttt gaagagtata
     6601 tgaaggcgca cgaagaataa gcaccgcgga tatacggcag ggcgcggcgc gccgggcaca
     6661 cgcccgcgcc gcgccttttt tgcgcctgcc gcaccgcggc gccgcccgga aaaacatgcg
     6721 aaagggagcg ataccatgga gcttttccgc tgcc
//
LOCUS       DAWBSB010000118         6751 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_2192_length_6751_cov_3.462814, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000118 DAWBSB010000000
VERSION     DAWBSB010000118.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 6751)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6751)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6751
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2192_length_6751_cov_3.462814"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2546..2555
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1165 a         2101 c         2026 g         1449 t
ORIGIN      
        1 gccagacacc tgtgcggctg gccaacgcca tcaatgtggc cgccgccgaa caggccgcgc
       61 ggcgggcggc acgcacacac gacggcgcgg cgcgcgccgc tttgtgcgaa cagtacagcg
      121 ccctggcctc ggctctggcc gagctggccg gacaggtgta ccagacggat gttccggaca
      181 agcgcaaggc ccgccggctg gagcaattgt tctcggagat cggcctggag ccgctggaga
      241 ccaccgttgc ccaggacgct tccggccgcg tgacggccac cgtatgcgtg ccgcgcctgt
      301 ccttcacggc cgacgagctg aaggtcatca ccgatgaggt ctccaccctg tgccgccgca
      361 ccttcgcgcc cgcccaatgc atccattccg gcacggtgac caagctgctg ttccgtgaac
      421 ggccccgctt tgccgtggac tgcgccgtct gcgccctgcc cgccggcggc gacatatccg
      481 ccgatgcgac acaggccttc cccgatgaaa caggccattt tcacgcggtg ctgtgcgacg
      541 gtatgggcac cggtaaggcg gcggcggtgg gcggcgtgct ggccgcctcc cttacgaaag
      601 agctgctgca cgccgggttc gaaagcgcca gcacggcgcg gctggtgaac atcgcgctgg
      661 cgctgaagag cgacgacgaa agcgccgtgg cactggacgc gctttccatc gacctgtaca
      721 ccggggacgc agtgctgtac aaggcggggg ccgcggcttc gtttctatta agggacggta
      781 aggccgccgt ttacagcggc gacacgcttc ccatcggcat tctcggcagc gtgacgggcc
      841 gccgggagct gctgtgcctt tcccccggcg ataccgctgt gctggtgagc gatggcgcgc
      901 tggctccggg cgcctcctgg ctgcgcgcgc agctcgtggc tcacgcggac gacgcgcccc
      961 aaaagctggc ggatgcggtg gcccgcgcgg cgagggaaaa gcagcaggac atgcccgacg
     1021 acgtaaccgt gatggcactg cgcctgcgcg cggtagaata accgccccgg ggcgggccgc
     1081 tcctgccgct tgcctcctcc gcgctcggcc gcggaaactt tttcttgatt cgctccatgc
     1141 cccatactat aatagagacc aaatacgact ggctctgtaa ggaggtgccg catggaagca
     1201 aatcaatatc tgaacgattt ttacaaccat tacgacgagg acagccgcct gctgtttcag
     1261 cacggcagcg tcgaatttct caccacgatg cgctacatcg agaaatatct caagcacggc
     1321 gacaaagtcc tcgagatcgg cgccggaacg ggccgctatt ctcacgcgct ggcgcgccgg
     1381 gggcatcccg tggacgcggt ggagctggtc gcgcacaaca tcgaggtttt ccgccaaaac
     1441 acccgtcccg gggaaagcgt caccatcgca cagggcaatg ctctggatct gtccgccttt
     1501 gcggacaacg cctacgatgt cacgcttctg ctcggcccgc tgtatcatct gtatactcag
     1561 gacgacaagc gccgggcgct gcgggaggcg atccgcgtca caaagccggg cggcgttgtc
     1621 tttgccgctt atgtcatctc cgacggctgc cttctggacg agggcttccg ccgcggcaat
     1681 atctctgttg ccgaatatgt gcgggacggg ctgctggacg cccggacgtt tgccgccagc
     1741 tcccagccaa aggatttgtt cgagctggtg cgcaaagagg atgttgatga gctgatgtcc
     1801 gtgttccctg taacgcggct gcactatgtg gcctccgacg gctgcgccct gcttttgcgc
     1861 gacgagatcg acgcgatgga cgctgacgcc ttcgccctgt atctgaaata ccatttcgcc
     1921 acctgtgaac gcgcggacct ggcgggcatt acaagccatg cggtggatat cttccgcaag
     1981 tgaccgactg tgtctgaatg ctgcgccagc tttcccgttc atctgacaca cggcgggaat
     2041 accccgccgg cccgtctgtt cattttaccg cttcaagctg tttccgcctt ctattacata
     2101 ggattgcagc cctttttgac atccgttctt ttcataaaaa tgctccaagc ccggctgcgc
     2161 gccaaaatac aacatttttg gagcgttttc cctggcaagc cggaggagtg tgctgccgat
     2221 tccctgtctt cgatattccg gaagaacgag aagctctgtg atcgtcccga aaaaacagcc
     2281 gtctgaaaga atgcgcaggc atcctgcaag ctttttctta tcataggcag ctatattgat
     2341 tgttttggac agcgcggccc gggttttatt ttcatcataa tttccaggcc atatccgatt
     2401 ggcaaactga ataaaggccg cagcgtccaa ttctttgtca tccactgtgt attccatcat
     2461 cttccccgct tttttcatac tttgaccgtt gtttgctctt ttatgaaaag ctccgggccc
     2521 ggctttccgc cgcacccgga gctttnnnnn nnnnngttat tttaaatgaa aaaggggttc
     2581 gccacccctt tttcaagctt tttccccaga catttctttt acttctctgc ctttttcaat
     2641 agtctgaaag ctccgggccc ggctttccgc cgcacccgga gcttttttct gtctgcgtgc
     2701 tgttgttact cgtacagcgg gaaacgggcc gtcagggcgg ctacctcggc ggcgacggct
     2761 tccttctgcg cctcaaaatc aaaggtgcag gccgcgatcc attttgcaat cttccgcatt
     2821 tcctccggcc cgaagccgcg ggtggtgaca gcgggcgttc cgatgcgcac gccggaggta
     2881 acgaaaggct tctccgggtc gttgggaacg gtgtttttgt ttgctgtgat gtgcacctcg
     2941 tccagacggc gctccagctc cttgcccgta acgcccgtgc cgcgcaggtc cacggtcatc
     3001 aggtggttgt ccgtgccgcc ggagacaagg cccagcccct cgtccagcag cgcttgggcc
     3061 agcacgcggg cgttctctgc gatattcttc tgatattctt tgaattccgg cttcagcgct
     3121 tcgccgaagc acacggcctt ggccgcgata atatgctcca gcgggccgcc ctgggtaccc
     3181 gggaaaatag ccttgtcgat ggccttggcg tactcctccc ggcacaggat caggccgccg
     3241 cgggggccgc gcagcgtttt gtgggtggtg gttgtcacca cgtcggcata gggcacgggg
     3301 ttcgggtgca ggccagcggc cacaagcccc gcgatgtgcg ccatatccac cataaacagc
     3361 gcgccgtttt cccgggcgat cttcgcgatg cgttcaaaat caatggtgcg ggagtaggcc
     3421 gaagcgcccg cgacgatcat tttgggctgg tgctcctttg cttttttctc cagatccgca
     3481 taatccagat agccgttttc gtccacgccg tatgcgatga aattatagtt cttgccgctc
     3541 atgttcacag ggctgccgtg ggtgaggtgg ccgccgttgg aaagatccat gcccaggacg
     3601 gtatcgccca cgttcagcag cgcaaaataa acggccagat tggcctgcgc gcccgaatgc
     3661 ggctgcacat tggcgtgctc ggcgccgaac agcctgcacg cgcggtcgcg ggcaatgtcc
     3721 tccaccacgt ccacgcactg gcatccgccg tagtagcggt ggccgggata cccttcggcg
     3781 tacttgttgg tgagcacgct gcccatggcg gccattaccg cgggcgagac gatattttca
     3841 gaggcgatca gctcgatatt gccccgctgg cgtttgagtt cggcctccat cgccgcgccg
     3901 acctcggggt cggcctgtgt gacgaagccg atggtgtcca tcatttcttt gaacatgggt
     3961 gtgccctcct gtactgtttt acttcctgcg gcggttttcc ccgccttctt cccaattata
     4021 gattatacac ccgccgcgcg cggcgcacaa gacgctttag cctgttaaac tgctgaattt
     4081 tcagcggcac agacgttccc ccttttgctt ttggacgaaa tgtgctatca taatatcaat
     4141 ttatcggcgg ctttctttta tgcgcgaaag cgcgcggggc ggccgccgtg caacagggag
     4201 gcgtcttttt gcgcacaaaa gcagatacca ggctcattgt agagcgcctg aaaaaggaat
     4261 accccgtgag cgaatgctcg cttgcctatg acgacgcgtg gcagcttctt gtcagcgtgc
     4321 ggctttcggc ccaatgcacc gacgcccgcg tgaatatcgt gacaaaggac ctgtttgcca
     4381 aatatcccac gctggaggcg ctggctgcgg ccgggccgga cgccatagag gccatcatca
     4441 agccctgcgg cctgggcaaa agcaaggcgc gggaccttgc cggcatggca aacatgctgc
     4501 tgcgggaata cggcggcaaa gtgccccagg ccatggagga tcttctgcgc ctgccgggcg
     4561 tgggccgcaa aagcgccaat ctcatccggg gcgatatctt ccacctgccc gccgtcgtgg
     4621 cggacaccca ctgcatccgc atggcaaacc gcatcggttt cgtgacggac accaccgacc
     4681 cggtgcaggt ggagcgcgcg ctggtgaagg tcctgccgcc tgaggagtcc aacgatttct
     4741 gccaccgctg cgtgatgcac ggacgcgtgg tgtgcaccgc ccgcaaggct ctgtgcgagg
     4801 tgtgctgcct gcaggacgtc tgccgcactg gcaaaaaaac catcaaacag gaaacattgg
     4861 aggaactggt atgaaacgca ctttatcgct tgctttgtgc ctgattttaa gcctggcttt
     4921 gctggccggg tgcggcgccg cgccgctgcc cgacggcctg acgcaggaaa gcgtgacgga
     4981 cgccgcggcg cagaccgtgg cgcagctgaa cgacgaggac tatgacgcgt tgttcgccgg
     5041 aatgacagag gagatgacct ccgccatgac ggccgaggcc cttgccgccg tatggcagcc
     5101 ggtgctggaa gccgccggcg cgttcgattc catcacaaaa acgaccgtgg gcggcaagga
     5161 cggttacggc gtggccgtgg tacaggcaaa atatgaaaac gccgcggtga cctttacgct
     5221 ctcctacaca ccggagggca agctgggcgg gctgttcttc aaataacccc ttccccgccc
     5281 cattcttttt tcgaggtgtt ccccttgcag cagacacagc aaaccaccgc gcccgtccag
     5341 aattctttct gggcgggctt tcacgacggc gtgcccattg gcctgggtta tctttccgtc
     5401 tccgtcacgt tcggcatgat ggccgtgacg ggcggcctgc cggtgtgggc cgccgtggcc
     5461 atcagcatga cgaacgtcac cagcgccggg cagttcgccg ggctttccct cattttcggc
     5521 ggcggcacgc tgtttgaaac ggcgctcacg cagttcgtca tcaatctgcg ctatgccctg
     5581 atgagccttt ccctctccca aaagctggag cgccgcgtcg gcctcatcga ccgcatgctg
     5641 ttcagcttta tgatgacgga cgaggtgttc gccgtggcgt ccgggcaaaa gggagaggtg
     5701 ggcaagcgct atctgttcgg cgtgatgatc gcgccttact gcggctgggc gctgggcacg
     5761 ctgtgcggcg ccgtggccag cggctttttg ccgccgtcgg tgcgcagcgc gctgtccatc
     5821 gccatttacg gcatgttcat cgccatcgtg gtgcccgttg caaagcagag ccgcgccgtg
     5881 ttgggcgtgg tggctctggc tgtcgcgctc agctgcgcca tgcactggct gcctgtgctg
     5941 aacacggttt ccagcggttt ttccatcatc ctctgcaccg tgctggcgtc gggcctgggc
     6001 gcgctggccg cgccggtgcc ggacgagcct tccggagcac cggacgggga ggtggcgtaa
     6061 atgaatgcaa aaaccatctg gctgtacatc gtggtaatgg cgggcgtaac ataccttata
     6121 cgcatgctgc cgctggcgct gctgcgcaaa aagctgcgca gccgttttat caaaagcttt
     6181 ctgttctatg ttccttacgc cgtgctggcc gtaatgacgg tgcccgccat ctttgagagc
     6241 acgggcagcg tgttttccgc caccgtgggc ttcgccgcgg ctgtgcttct ggcttaccgc
     6301 ggcgcggctc tgctgcctgt ggcattggcc gcctgcggcg tggtattctg cacggagtgg
     6361 atgatgcgcc tgttttagcc cataaaagag gaattcgcct atgaattgga tcagctggtt
     6421 cggccttggc atcgtgttgc tgctgctcat tcccaacgcc gtatacgccg ccgcaaacaa
     6481 aaacacgcaa ccgccccgca caagccgcat cctcggcttg gctgaacagg caggccgcta
     6541 tggatgcatg tttctgatga tattccacgc cggccttacg gaattcggtt ttgcctcggc
     6601 ggaaggcttc atcgcgtggc tggccggcac cggtgcgctg ctcgtactgt actgggtatt
     6661 ctggctgctg tatttccgcg ccgcgaagcc ccggtacgcg ctgccgctgg ccgtgctgcc
     6721 ctgcctgatc ttttttgctg aacggccttt t
//
LOCUS       DAWBSB010000119         6714 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_2204_length_6714_cov_3.467187, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000119 DAWBSB010000000
VERSION     DAWBSB010000119.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 6714)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6714)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6714
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2204_length_6714_cov_3.467187"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3229..3238
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5827..5836
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1150 a         2020 c         2321 g         1203 t
ORIGIN      
        1 gcggaaagac cgtgcgcatc gggccgggac gcggcgcggc cgcgcaaaaa ttaccgggag
       61 cgtgtaaatt atggaaaaat atgcccttat ggcggcggcc ttcggcgcgc tggcgatcac
      121 agcggcctcc ggcctgctga tcatccccgc gctgcgcagg cttcacttcg gccagaccat
      181 caaggagatc ggccccacct ggcaccaggg caaaaacggc acgcccacca tgggcggttt
      241 gacgttttac ctgggtgtcc ttgtgggcgt ggtgctgggc tatacggttc tggcgctcag
      301 cgtgcccagc ctgctggggg gctggctttc cggcaccagc gtctcgctgt tcatcggcgt
      361 gctgacggct tacgccttcg gcctggtggg ctttgtggac gactatatca aggtggtgaa
      421 aaagcgcaac ctgggcctga tggcgcgcta taagatcgtg gcgcaggtgc tcatcaccgg
      481 ggcgtacctt tcctcgctgt atctcaacgg aacgctgtcc accatcgtga cgctgccgct
      541 gctgggcgcg gtggacttcg gctggttctt ttacgtcctc tcgttcctcc tcatcatcgg
      601 catggtgaac gccgtcaacc tgacggacgg catcgacggg ctggcgtcca gcgtcacctt
      661 tgttgtaatg atgggcttca tgtttatcgc cagccttttg ggaaacacta ccatggcgct
      721 gtttgccgcg gccgtggcgg gcggctgcgc gggcttcctt gcgtggaact tttacccggc
      781 caaaacgttt atgggcgata cgggcagtat gttcctgggg ggcgcggtgg tggccgtggc
      841 ttacggcatc ggccggccgg agctgctcct tttcatgggt gttatctacc tgctcgaggc
      901 gttcagcgtg atgctgcagg taacatattt caagctgacc cacggcaagc gtatttttaa
      961 aatgagcccc atccaccacc actttgaaat gtgcgggtgg agcgaggtga agatcgtctc
     1021 cgtgttcagc ttcatcacgc tggtgggcgt ggtgctgggc ggtatttttg tctacgtatc
     1081 ctgattcccc aataagcagc ctgtgccgat gcggccggcg cgggcaattt tgtatcttcg
     1141 gaggttcccg atgccagaag cagccaaaaa gttaaaacgc aagtccgagg cccgcatccg
     1201 gctgctcagc cagccgccgc tggactggcc gctggccatc atcgtgctga cgctggtggt
     1261 gttcgggctg gtcatgctgt tcagcgccag ctatgccacg gcgtattacc gcttcaaggg
     1321 cgacagcttc cacttcatcc gccagcaggc gctgttcgcc gcggtgggcg tggtcgtgat
     1381 gttcgtcgcg tcccacgtgg attaccacat cttccacaaa tttgcgtggc ccatcatggc
     1441 cgtcacggtc gtactgctca tcgtcgtgct cttcatgccg gagctgaacg gcgcgaagcg
     1501 gtggatattc ctgcccatcg gcggcaatct gcagccctcg gaggtggcga agttcgcggt
     1561 catcgtgctg ttcgcgcaca tcatttccat caaccataag cgcatgaaga cctttgccta
     1621 cggcgtgctg cccttcgtcg tcatcctcgg cgtgatcgcg gggctgatgc tgctggagcc
     1681 gcatctttcg ggcacggtgc tcattttggg catcggggga ctgatgatgt ttgtgggcgg
     1741 cacggggctt gtatggttcg gccttgccat cgcgggcgtt gtggcgggcg tggcgggcgc
     1801 cgttgtgctg gtgccggacc ttgtgcccta cgccatgagc cgcatcgaaa gctggcagaa
     1861 cccgtggctg gacgcccagg gcgacggcca ccagatcatc cagagcatgt acgccatcgg
     1921 ctcgggcggg ctgacgggcc ttggcatcgg caactccaag cagaaacacc tgtacctgcc
     1981 cgagccgcag aacgacttta ttttctccgt catctgtgaa gagctgggct ttatcggcgc
     2041 gctgctggtg attttccttt ttgtggcgct tttgctgcgg ggcatttatg tggcgtggca
     2101 cgcaaaagac aaattcggct ccatgctggt ggtgggcgtt atcgtgcagg tgacgctgca
     2161 ggcggtgctg aatatcgccg tggtgacgaa gaccattccg aacacgggca tcagcctgcc
     2221 gtttttttcc tacggaggca cgtccctgct gatgctgctg ggcgagatgg gcgttgtgct
     2281 ttcggtctcc cgccagggcg gcgcggagaa ggtatagcgg cgcgcggcag ggccgcgcgt
     2341 ctgcattttt attttttgag cgggggcaga ccgccggtga aaagttttcc ggcgcggccc
     2401 ggcgcctgga accgtgcggt attgcgcgaa agcaggaggt acctatgcgt gtattgatcg
     2461 ccgccggcgg cacagccgga cacatcaacc cggcgctcgc cattgcgggc gcgctgaaaa
     2521 aacgccatcc ggacgcggag atacacttcg ccggacggca gaagggaatg gagtacggcc
     2581 ttgtctccaa ggcgggttat gcgttccatc ccatcgaggt gaacggcatc cagcgccgcc
     2641 tgacgccgaa gaacatcgcg cggaacctgg ttgccctttg gcacctggcg ctgagcggcc
     2701 cgcgcgccgg cgccatcctg cgggaggtaa agccggacct tgtcatcggc gcgggcgggt
     2761 acgtcagcgg gccggtggtg caggccgcgg cccgcagggg catccgcacg gccattcacg
     2821 agcagaacgc tttccccggc gtgaccaaca agatcctggc aaagcaggtg gacctggtgt
     2881 tcgcggccat gcccgacgcc gtgcagcgtc tgggcgcgcc ggacaagacg gtcgttacgg
     2941 gcaacccggt gcgggaggag atgttccggc aggaccgtcc cgccgcacgc cgcgcgctgg
     3001 gcgcgaagga ggggcaggcc gtcatcatct ccttcggcgg cagcctgggc gcgcaggtgc
     3061 tgggcgaacg cgtggccgat ttggcaaaat gggagatcgc gcagcgtgat ttccttcatg
     3121 tgcatgcgac gggcagcatt gaaaaagcgg attttgcagc ccttgccgcg cggctgggca
     3181 tcgaccacag cccgaagttc atcatccggg agtacatcga caacatgcnn nnnnnnnncg
     3241 gtgctcatcc cgtcgcccaa cgtggcggaa aaccaccagt accacaacgc catgcagctg
     3301 cagaagctgg gcgcggccgt tgtggttgag gaaaaggagc tgacgggcga gaagctcatc
     3361 ggcatcgtcg acaagctgac gaaggaccct gcggaattga tgctcatggg ggcgaaagcg
     3421 cgtgcgctgg cccagccgga ttcgctggat aagatctgcg aaaatctgga ccgattgatg
     3481 aaaaccacgg cgtaaacggc gcgcttttaa cacaaacggc cctgtttgca taggatgggt
     3541 tacagtctgg gacactgggc tccattctac gaaacggggt gcgggatatg gaaattttga
     3601 cgatcagcgg aggcagcggc ctttttggag aggtgcggat acccggcgca aagaacagtg
     3661 tgctgcccat catggcggcg tcggtgctgt gccgcggcac ggtgacgctc acggacgtgc
     3721 cgcatctgtc cgacgtggag gcttcggcgc gcatcctgca aagcgtgggc tgcaaggtcc
     3781 tgctggagaa tgggcgcgcc gtcattacgc cgccggaatt tccgggcagc gcagtgccgg
     3841 agccgctgat gcgcgcgatg cgctcctcgc tgttctatct ggcgccgctg ctggcccgca
     3901 cgggccgcgc ggacatctat acgcccggcg gctgccgcct gggcgcgcgg cccattgata
     3961 tgcatctttc cggccttgag aagatggggg cgcatatcac ggagaaggac ggcggcgcgc
     4021 tggaggtcac tgcgccggac ggccttgcgg gcgcggatat ctgcctgcgc ttccccagtg
     4081 tgggcgccac cgagacgctt ctcatggcgg cggccgcagc cagaggagag acggtgctgc
     4141 gcggcgcggc cgtggagccg gaggtgacgg atctggcgcg ctttttgcag ggcgcgggcg
     4201 cggatatcac aggcgtcggc acgcgggtgc tgcacatccg cggccgggag ctgctgatgg
     4261 gcacgcagca tgccgtctgc ccggaccgca ttaccgcggc caccgtgctg tgcgctgtgg
     4321 cgggctgcgg cggcgaggtg ctgctcacca atacctgcaa cgcccatctg gccgaggtgc
     4381 tgcgcatcct gcgcagggcc ggatgcatgg tgtcggcctc gggggacagc tctgtggcgc
     4441 tggcaagctc gggcgctctg cgcgccgcgg gagggtggga tacgggtgtg tatccatcgt
     4501 tcccaacgga cgcggcaccc ctgatggcgg cggcgctgct gcgggcgcag ggggaaagcc
     4561 gtgtgacgga cacgattttt gaaaaccgtt ttgcctgcgc cgaaggcttt ttgcggctgg
     4621 gtgccagcgt gaatgtttgc ggccggacgc tgcatgtcca cggcatggac ctgctgaacg
     4681 gagcggacgt ggaagcgccc gacctgcgcg gcggggcggc gctggtcatc gccgcgctgc
     4741 aggcacaggg cagcacccgc gtcatgggcg cggagcatat cagccgcggc tacgaggaca
     4801 ttgcggcgct gcttgcaccg ctgggggcgc gcatcacgcg cagctggggg tgaaccgtgc
     4861 gggcgcggtt gccggaatgc cgggaccgtg ctatgataat agaaaactcc gggccggggg
     4921 agaagggccg gccggtccgc atccgcacgg ccgtgcggcg gcaggggcag gtgaaacaaa
     4981 aagagctccc gctgcatgcg gcgccgggcc gcaaagacgt tgacgagaaa ggagtggcgc
     5041 gttttggcac gcagcacgcc gccgcagaaa cccgtttatc caaaaaacga accgcagccg
     5101 cgccgccagg tgccgcagcg gcccgtgtat acatacgatg cggatgtgcc caggcagccg
     5161 cgcaagcccg cgggcgggcg agtcgtgtac gattataaca aatcggcagg ggcgtcctcc
     5221 ggcgtgcccg ccggttcccc ggcccgtcct gcggctccgt tcgtgaatac ggcgccgcag
     5281 gccccgcagc aggggcgggg aaccgcgccg gccgccggaa acagcgcggg cattccggag
     5341 ccgcggcagc ctcgcgacgg gggcccgccg gcccgcggcc gggaggaaat atacggccgg
     5401 ccgccgcgtt cccaggcaca ggccggggca caacggcgcc cgcagcagga cgtgcgccgt
     5461 acgcctcagc gggaagagcc gcgctggcgc gctctgccgg aacaggaggc ggagctgaag
     5521 ccgcgcccgg gcgtgtacga ccagagcgcg gacaggcccc gccaaccccg cgcaaacagc
     5581 gcttcgcccg gcccggcccg cacgccgggc gggccgccgc cggggaaaag cggaaaggcc
     5641 cggaaaaagc ggaggaaaaa gcgtccgccc cgccccctca caccgggcga agcgcgccgc
     5701 cgccgcgtcc ggcgcggcat tctggcgggc gtgctgatcg ccgtgctgct ggcggccggg
     5761 ttcctgatct cggcggccgt gctgtttaag atcgagacag tcaccgtcga atctcccgac
     5821 gggcagnnnn nnnnnnggcc tacgacgaca gccagatcgt cgcggctttc ggccagccgg
     5881 cgggggaaaa cctgttcggg ttcaatacgg cggaggcgca gcaggccatc gccgcggcgc
     5941 tgccgtatct ggagagcgtg gagatcaagc gccgcctgcc ggacaccgtg gtcatcacag
     6001 ccacgcccgc ggtggagacc tacaccgtgg agagcgcggc gggctgggcg gttctgagcc
     6061 aaagctacaa ggtgctgcgt gtggatgccg aggcgcccgc ggggcttgtg cgcatcgacg
     6121 gcgcgcaggc cgacgcgccc gcgccgggcc agccggtgaa gtttaccgag gaggacaagc
     6181 tgtccgtcct gcagaccgtg ctttccaagg cgcaggcgca ggggcttggc cccatcagcg
     6241 aggtggacct gacgaacacg ctggaactga gcttcctgta tcaggaccgc atccgcatcg
     6301 tgctgggcac caccaacgac ctggattata aaatcaaatg ggcgtgggaa atggtgaccc
     6361 ccggccagac cagcgacagc ctgggcgagg aagagcgggg cactctggac gtgagctccc
     6421 gcggggagga cggccttggc cgcgcccgct ggcgcgcggg cgtgctgtga gagcaccggg
     6481 cgtaaccgac tatatatact ttatttttac ataatcttgg aatggctatt gaaatcaaat
     6541 ctgaaatctg gtatcctaat acttagtgta tagtacaaaa ttgtctttag ggggaattct
     6601 cgtggcattt atcctcgatg aagaactgca gaatataacg aacatcaagg tcatcggcgt
     6661 gggcggcggc ggcggaaacg ctgtcaaccg catggtggaa agcggcctgc gcgg
//
LOCUS       DAWBSB010000120         6700 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_2209_length_6700_cov_3.369752, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000120 DAWBSB010000000
VERSION     DAWBSB010000120.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 6700)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6700)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6700
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2209_length_6700_cov_3.369752"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1212..1221
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1546..1623
                     /estimated_length=78
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6052..6061
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1340 a         1867 c         2055 g         1340 t
ORIGIN      
        1 ccgcttgcac agcgcacgct cagaacggct ccagcccgag aagatagtcg gcgctgacat
       61 gataatagcg gcacagggca atcaggtgac ggacgggcaa ctcgttttcc ccgttttcat
      121 aacgggaata tacctgttgc gttgtgccga ggtattgggc tatctctttt tgggtcaaat
      181 cccggtcttc ccgaagctcg cgtatgatgt ctacataagt tttcacgcaa acacctccgc
      241 gcccatttta ggctacacct gattaagtgt attgactgta cactatatgt ggtgtaaaat
      301 aagggcaaac actggaggag gcattgaaaa tggaagtaga agcaaatacg tctgggcaga
      361 cgcaggccaa aacaaagttt tgcaagcact gcggtggtac gatccctcag gatgcggtaa
      421 tgtgtacgca ttgcggcagg caggtggagg agctggccgg cgcggcacag ccgcaggtcg
      481 ttattcagaa taccaatatg aacaccaata cagcggcggc agtggttgcc ggtgtagggg
      541 caagggcaaa aaacaaatgg acggcgtttg ttctgtgtct gcttttgggt tactttggcg
      601 cgcacaaatt ttacgaggga aaggtcggga tgggcgtact ttatctgttt acgttgggcc
      661 tttttgggat cggatggttt gtcgacctta tcgttctgct cacaaaacct aacccgtatt
      721 atgtttagag cggcgccgct ttatcgcgtt aaaatgtttt aaaaaatttt gcggaaaccg
      781 cttgacattt ttgccggttt ggtgtttaat actaccaaac cgatgaagcg gagataaagc
      841 cgtaagcgca cgcaaagaga gcccccgttg ctggaagtgg ggccggaagc ggaacggcaa
      901 atggaccgcc gagggcaggg tgaaaggcgc aaagcccagt agcttctgac gcgtgctcgc
      961 gttacagggc agaggatgtg ttggcatctt cgcagaggcg gccgctgttt gagcggcaat
     1021 caaggtggta ccgcaggtgc agtttcagcg cctgtccttg tgaacagcaa gggcaggcgc
     1081 ttttttattt gcgccggccc ggcgcaggag gggttcccaa tgaagaaaaa accgaatctg
     1141 ctttcaaaac acacccagcg gctgcgatgg cgatggtgaa acgaagcctt gtccgcccat
     1201 atgcccggcg annnnnnnnn ncgcctgcgg cggttcgtcc tcctccggtt ctgtcccggc
     1261 tgcggcctcc gcgtccggtt ccggttccgt ttccggagct tcgctgaaag aagtcaacat
     1321 cgggctgatc cagctgatgg agcacgcttc tctggacgag atccgcacgg ccatcgaagc
     1381 cgggctggag gccaaagggg cggaatacgg cgtaaaagtg aatgtcgatt accagaacgc
     1441 ccagggcgac accagcacca tcaacaccat ctgccagcag tttgtgggca acaaggtgga
     1501 cgccattgtg gctatcgcaa ctccggccgc gcagggcgcg gccacnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnngtcttgt ggaaaacctg gaggcgccct agggaaacat cacgggtacc tccgacgcga
     1681 tccccgttga aaaaatcttc gagcttgccg ccgagcttac gcccgacgcc gagagcttcg
     1741 gcctcatcta caacaccggc gaggtgaaca gcgtttccgt aatcgacgag acaaaggcct
     1801 atctggacgc ccagggcatc agctacaccg aggccagcgt tgccagcacc ggagacgtgc
     1861 agacggcggc acagacgctg ctttccaaat gcgacgcaat ctttgcgccc atcgacaaca
     1921 cggtggccag cgccatgggt gtgctggcgg acgaggccat caaggccgga aagcccgtgt
     1981 atgtggcggc cgacagcatg gtgaacgacg gcggccttgc caccgtgggc gtcaactaca
     2041 ccaacctggg cacccagacg gccgacatgc tgctgaaggt cctgacgggc acgcccgtct
     2101 gcgaagtgcc tgtggaggtc ttaaaggaga acgccgttgt ggtgaatgaa gagaccgccg
     2161 ccgccatcgg cgtggacgtc agcaagtacg cccggtaagc tgcacgcgga aagggacact
     2221 gcaatgaaaa acagaatatg cgccgcactg ctcacccttg tgctgtgcct gcctctggca
     2281 ggctgcgcgg gcggggcttc ctccggtata tccgtttcct acccggcgga gccctcttct
     2341 ggtgcggatt ccggtatgca ggccggaaag gcctataccg tgggcatcct ccagcagatg
     2401 gaacacacct ctctggacga gatccgggag gccgttgagg cgggccttgc ccggggcgcg
     2461 gctgcgggcg gctatacggt ggaggtcgtt tataaaaacg cacagggaga tcccacagcc
     2521 atccgcacca tcgcggagca gttcgccgca cagggcgttg acgtgatcgt gcccattgcc
     2581 acgggtgcgg cccagggcgc ggctgcggca gcgcaggatg tgcccattgt gttcagcgcg
     2641 gtggccgacc ctgtcgcggc cggactgttg gatgcttttg accggacgac gggcaatatc
     2701 acgggtgttt ccaacggcat tgacgtcgca aaaatattcg ctttggcgga cgagctgacg
     2761 cccggcatcg ggacctacgg ctttatatac aatgaaggcg aggtcaacag ccagtccatc
     2821 attgaaaaag caaaggcctt tctggatgcg cggggcctcg cctatgagga agtgacggtc
     2881 gcatcctcgg gcgaggtgca gcaggccgcg cggggcgtca ttgcaaaggg cgtggacgcg
     2941 ctgttcatcc ccaacgacaa cacggtggcc agcgcgctgc ccgtgctggc gcaggaggct
     3001 attgcggcag ggcttccggt gtatcccact gcggacagca tggtgcggga cggcggcctt
     3061 gccactgtgg gcatcaatta taccgtgctg ggcggccaga cggcagacat ggtgtgcaga
     3121 gtgctggagg gcacgcccat cgcggatatc ccggtggagc tggtgaatga gtcctccgtc
     3181 gtggtcaacg gggataccgc ggctgcgctg ggtgtggatg tgagccggta cgtgcagtga
     3241 cacgctgaat atgctgcaat tgaaaaaacg ggccgcgctg cggcccgcgg cggaacggag
     3301 cggcctttaa atgccgctcc gcgccgcctt gttccggaaa atacatacaa aaggggaaac
     3361 gaccatgagc ttgcttgttt tgcagggcgc cgtggagcag ggctttattt atgcccttgt
     3421 cgcgctgggc ctgtatatct cgttccgtac gctggacatc gcggacctca ccactgacgg
     3481 caccttcacg ctgggcgcgg ctgtatcggc tgtgtttact gtgcagggcc agccgctgct
     3541 gggcgtggtg ctggcatttt tctgcggcgc actggcgggg tttgtcaccg cgcttttgca
     3601 gacgaagctg ggcgtgcagc ctattctggc gggtatcatc accatgactg cgctgtattc
     3661 cgtcaacctg atggtgatgg gaaagccgaa tatcaatttc tttaaggaaa agactatctt
     3721 tacggccgcc gaggccatgc cgggcggctt cggaagcctg ctgcaggccg cggccatcac
     3781 cgccgtgctg tgcgtgctgc tggtgctgtt tctgcgcacg cagctgggcc tgtctctgcg
     3841 ggccacgggc gacaaccggg acatggtgtc cgcatcgtcc atcaacccgg cgttcaccac
     3901 caccgtgggc ctgtgccttt ccaacgccgt ggtggcgctt tcaggtgcgc tcatcgccca
     3961 gtatcagaaa tttgctgaca atacgctggg tacgggcatg gtagtcatcg ggctggccag
     4021 cctgatcatc ggcgaggtgc tgttcggccg cggcgggccc cacgcgctgg caaaaggcgt
     4081 gctggcggca actgtgggcg ctgtggtata ccgcatcatc gtggcggcgg ccatcgcggt
     4141 gaacatcgac gcaaaaaaca tgaagctggt ttcggcgctc atcgtggcgg cggctatttc
     4201 gtacccggcc atccgggaca aggtgctctt ttacagaaaa cgcagggagg cgaaccgcaa
     4261 tgtctgaaat gctggaactg cgtcatatct ccaagacctt cggcgcgggc acgatcaatg
     4321 aaaaaaaggc tgtggaggat ctttccctca cactggcctc cggagatttc gtcaccatca
     4381 tcggcggaaa cggcgcgggc aaatccacgc tgttcaacgc tgttgcgggc gtgttctatg
     4441 tagacgaggg ccgcgttatc ctggacggag aggatatgac gttcctgccg gaatataagc
     4501 gctccaacca cctgggccgt ctgtttcagg acccgctcaa ggggaccgcg ccccacatga
     4561 ccatcgagga aaacctggcg ctggcgtatc tgcgctctgt gcgcacgcgt tcgcctttcg
     4621 gcacggtgtc gaaaaaggac agggagtatt tccgcgagcg gctggccgct ctgggcctgg
     4681 gcctggagga ccggctgaaa agcccggtgg gcttgctttc cggcggacag cgccaggcgc
     4741 tgacgctgct gatggcaacc cttgttacgc ccaagctgct tttgctggac gagcacaccg
     4801 ccgcgctgga tccctccacc gcagaaaagg tgctggagct gaccaggagc atcgtggcgg
     4861 aaaacaacat tacatgcatg atgatcaccc acaacatccg ctccgcgctg gagcttggca
     4921 accgcaccat tatgatggat gcgggccgca tcgtgctgga ccttgcgggg gaggagcgcg
     4981 cgggcatgac ggtggacgcg ctgctggaaa aattccgtac gagcgcgggc aaagagctgg
     5041 acaacgaccg catcctgctc agcgacaggg agtaaagtct gcggccggta tttgtgaaaa
     5101 aaattaaatc gtataaaaat agccgcagac tattttttag accatgtggg ccgtccccgg
     5161 cccgcggccg ctaaggtgtt ttttgttaca aagcaataaa aaggcgtccg aagatgaagt
     5221 ctccggacgc tttttctcgt attataaaca gaaggctcat ttgccggttt cggcctgcgc
     5281 tttcagcagg tcgcggatct cggccaggag cgtttcttcc ttggagggct ccgctgctgc
     5341 ggcagcctcc acgacctctt ccgctttctt gcgcttgaag cggttgatga ccttcagcgc
     5401 gcagaacacg cacagcgcca gaatgatgaa ttccagcacg ctctgcagaa aagcgccgta
     5461 gggcagggaa aagtaaggcg cttcttcgcc ggcccgctgc agcgtaacgg cgagctcgct
     5521 caggttcacc gcgctggtga acaggcccac aacggacatc accatattga cgagcgcggt
     5581 cacgatcttg ctgaacgcgc cgccgatgac gatgccgaca gccatgtcga tgacgttgcc
     5641 gcgcaccgca aactccttga actcggtgag aaattttttc atgcttgcct cctttgcctg
     5701 ttacagcagc ttttcgatgg cgtcggccgc gccgtccagc atgtcgccct caaaggattc
     5761 gatcacaccg aggtcggcct gcctggcaag ctgcgggtcg atggggatgc gggccagcac
     5821 cggaacgccg aactcggcgg ctgtctcggc aacgtggctc tcgccgaaca cgtcgatttt
     5881 tttgccgcag tccgggcaca ggacatagct catgttttcc acaaggccca gcacgggaat
     5941 gttcatcata ttggccatgt tcacggcctt ggcgacgatc aggctcacca gctcctgcgg
     6001 gctggccact acgacgatgc cgtccaccgg cagcgtctgg aacacggtca gnnnnnnnnn
     6061 ncaggaaatc cacatccttc cagatgacct cctgccagaa ctgcttgaca gtgcccgcaa
     6121 tgatgggccc gcgccacacg acagggtcgc tttcgtgctc cagcagcagg ttcacgctca
     6181 tcacgtcgat gcctgtgcgg ctctgtacgg gatagcagcc cacttcgtcc gcatgggcgc
     6241 ggccgtggat accgaacagc ttagggatgc tggggccggt gatgtcggcg tccagcacgc
     6301 cggtcttata gccgcggcgg ttcagcagca cggccagcat ggcggatgtc atgctcttgc
     6361 cgacgccgcc cttgccgctg acgacgccga tcactttttt aatactgctc agcgcgtggg
     6421 gcggttcgcg gaaatccgcg gggtttgttt tgcgcgaggc gcagttttcg ccgcaggtct
     6481 cgcaattgtg cgtacaattt tcgctcattg tcattctcct tccgggtggt accttcatta
     6541 taggttcgcg caaagagggt gtcaagccgt ggctttggtt gcgggacagg gaaatatacg
     6601 gtataatatg tccggttagc gaatgcgagc cgcaggcgaa gcatgcgctt agcggcatag
     6661 ataccatgtt tcttagtgaa cacgagcggt acgcgaagtg
//
LOCUS       DAWBSB010000121         6665 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_2224_length_6665_cov_3.386233, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000121 DAWBSB010000000
VERSION     DAWBSB010000121.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 6665)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6665)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6665
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2224_length_6665_cov_3.386233"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1285 a         2154 c         1974 g         1252 t
ORIGIN      
        1 ccgcgctccc caaagccccg ccgctcaccc ttccagctca aggtcccgca tcgcgggatt
       61 ttcgatgagg ccgccctcct gcgtgaagcg cgagccgtgg caggggcagt cccacgaatg
      121 ctcctgcgcg ttccatttca gcgcgcagcc catgtgcggg cagcgcggcg ccgtgggcgt
      181 gagcaggctt gccgcggcct ccagcgcgtt cagcgcaagc tgcgggcgca gcatgctgcg
      241 ggagggggaa aacaccgggg cgtcgtccgg ccttttgccc tgcaccatat ccgtgagcag
      301 catggccgcc gccatcgcgg aggtcattcc ccatttgttg aagcccgtgg ccacatacag
      361 gttttccgtg cgggccgaat acgggccgat ataaggcacg ccgtccagcg tcatgcagtc
      421 ctgcgcggcc cagcggtacc gtatgtgggc gcggggataa tgccgctttg caaaggcttc
      481 cagctcccgc catccgccgc cctgctttcc cgtgcggtgg ctgccgccgc ccagcagcag
      541 cattccctgc gcgttccgga aggacaggcc ctccgcggcc tcgtccacat acataccgcc
      601 cacgttcgcc gcgttttcca gcgcaagcac ataggagcgg tgctggtaca tcttcagaaa
      661 atagcttccg tgccggttga ggaacggaaa atgcgtcgcc acgatgacct tctccgcgcg
      721 gatcgtcccg cggtcggtaa cggcgctcgt cccctccagc ctgcgcacgg gcgtgtgctc
      781 gtaggcccgc agccctctgg ccgcggccgc ggcaaagcgc agcgggtcga actgggcctg
      841 gcgctcgaac cgcacggctc ccgccaccgg aaaaggcagc ggcagcttgt ccgtaaagcc
      901 cgcgtggaac cccagcttct ccagcgccgc aagctccttt tccagctttc cccggtcgtc
      961 cagggaatag acaaacgcgt ccttttcctc gaacccgcag tcgatctccc ggcacatgcc
     1021 gcggtagcgc gccagtgccg cctcgttggc gtgcaggtac atccccgcct tttccgcgcc
     1081 gaagcggcgc agcagcccgt cgtataccag cccgtgctgg gacgttatct tcgcggtggt
     1141 gttcccggtg gtgccggcgc ataaccggtc cgcctccgcc agcacacaat ccatgcccgc
     1201 ctgccgcaaa aaatgcgcgc acaaaatccc cgccatcccg ccgccgatga ccaaaacgtc
     1261 cgtccggacg tccccctcca gccgcccgta ttccggcatc gccgccgtgt cccgccacat
     1321 cgaaagcatc cgttttcccc attccgtccc tgtttttgtg cggcgcccgc gtatcatgcc
     1381 gccacggata gtgtttaccg cgcgccgccc cctatgcggc gccgtgcaaa aacgccctgt
     1441 cttccgccgc aaaagcagaa aacagggcgt tttcggagta tgcacgccgc ggcgctcaca
     1501 gcagcaccac gcagttgcgt ccgttctttt tggcaatgta cagcgcgcgg tccgcacggc
     1561 tcacaaggtc ctgcagcacg ggcgcgctgc cgtcccgcgc cgggctgtac accgttacgc
     1621 ccacgctcac cgtcagcggg cccgcgcagc agccgtccgg cgcacagggg ccaagctcct
     1681 ccacggcgcg gcgcaggcgc gctgcctggc gcatggcttc ggccgtgtcc cggcagggcg
     1741 tgcacagcag aaattcctcg ccgccgaacc ggcataccat gtccgaggag cgcgccagca
     1801 cgctctgcag tgcgccgccc acgcggcaaa gcgcgccgtc gcccgcaagg tggccgtgcg
     1861 cgtcgttgta ttccttgaaa tggtcgatat ccgccatcag aacagccatc ggcttttcgc
     1921 agcggcggca catggccagc atggtctccg ccttttcctg cccgccccgg cggttcaaaa
     1981 ggcccgtgag catgtcggtg ccgcactccc gccgcagctg ttcaatgcgc cgttcgtccc
     2041 ccgcccgcgc tctgcgcgag agcacgcgcc ccgtggcgta tgccgcaaaa acgaacgcgc
     2101 ccgcgcgcag cgccgcggcc agcacacacc ggcccgcgcc tttgtcatat ctgctgatga
     2161 tcgctcccgc cccttttcaa atttccacat attcccattt tcatcccttt gattgtagca
     2221 ggaagcaatg tcgaaaaagc gcaaagcatg ccctgcataa aggtgagcat acagaagaaa
     2281 accggaggat ttcaaagcaa aacccgcttt ttttacggaa tccacaggaa aaacagcgtt
     2341 ttttcgcggt ttctgcaaaa atattgcgaa aaaaatgggt aaaatatatt gacaaaccgg
     2401 caagcggccc gtatagtatc atttgtgcgc tgcaaagcgc agccatctta acatacgcca
     2461 gcgtaacatg cgccgctgca gccgctcctt cgggcggtgt aacaaacctt ttgcggggtg
     2521 gcgctctctt cgctgggccc ggggcttccc cgggaaaagc cgcaaagggg gatgcatatg
     2581 gacaattcta tcatcgttgc actgaaaaac attgttgtcg atttcgacgg cgagcccatt
     2641 ttaacagggc tttccctcga catccatgac aaggagttcg tcacactgct ggggccttcg
     2701 ggctgcggga aaacgacgac gctccgcgtc atcggagggt tcgtggagcc gagatcgggc
     2761 aatgtttttt ttgcgggcag ggacatcacg cgcctgccgc cgtacaaacg gcaggtcaac
     2821 accgtgttcc agaaatacgc gctgttcccg cacctgaacg tgttcgagaa cgtggcgttc
     2881 ggcctgcggc tgcagaagct ggacgaaaaa gagatccgct cccgctgcct ggagatgctg
     2941 gagctggtgg gcctgaaggg ctacgagcgg cgcaacacgg ccgcgctctc cggcgggcag
     3001 cagcagcgcg tggccatcgc gcgggcgctt gtgaaccatc cgaaggtgct tttgctggac
     3061 gagccgctgg gcgcgctgga cctgaagctg cgcaaggaca tgcagatcga gctcaagcgc
     3121 atccagcaga gccttgacat cacgttcatt tacgtcaccc acgaccagga ggaggccctc
     3181 accatgtcgg acaccgtcgt ggtgatgaac cggggcgaca tccagcagat cggctcgccc
     3241 caggatatct acaacgaacc gaacaacgcc tttgtggcgg atttcatcgg cgaatccaac
     3301 atcctggacg gcgtgatgct gcgggacttc cgcgtggaat tcgccgggca ggaattcacc
     3361 tgcgtggacg cgggcttcgg cagccgggag atcgtgcagg tggtggtgcg cccggaggat
     3421 atccaggtgg tgcccgccat tcagggccag ctgacgggcc tggtggaaaa cgtcatcttc
     3481 aagggcgtgc attacgagat gcacgtgcgg caggcaggct acgagtggat catccactcc
     3541 acacaggcgt cgcaggtggg cgaactcatc ggcatgaaca tcgggccgga cgagatccac
     3601 atcatgcacc gcacgcaggg gtgacggccg atgaaaaaga caaaatggac cgcttcgccc
     3661 tatctggtat ggatggtggc gttcatcgcc gtgccgctgg ccatcgtggt gtatttcgca
     3721 ttcaccgata aagccgggca ttttacgctg gaaaacatca tgggcctggg cgcgtacacc
     3781 acggtgtttg cccgcagcat cgtcctggcg gccatcgcca cggccatctg ccttgtgctg
     3841 gcctttcccg tcagctacct gctctcccgc atgcggcccg caagccagcg catgatgcag
     3901 atgctcatca tgcttcccat gtggatgaat tttctgctgc gcacctatgc gtggatgagc
     3961 cttctggagc acaacggcat catcaaccgc tttctggggc tgctgggcgt cgggccgttc
     4021 aacatgatca acacctcggg cgcggtggta ctgggcatgg tgtacaatta cctgccctat
     4081 atggtgctgc cgctgtacac catcatggtc aaaatcgaca acagcgtcat cgaggccgcg
     4141 caggaccttg gcgcaaacgt gtaccacgtt gtggcaaagg tgctggtgcc gctttccatg
     4201 ccgggcatca ccacgggcat caccatggtg ttcgtgccca gcatctccac gttcatcatc
     4261 agccgcatgc tgggcggcgg ctcgaacctg ctcatcggcg acctcatcga gctgcagttc
     4321 ctgggcaaca gctacaacta caatctcggc agcgccatga gcctggtgct gatggtcatt
     4381 gtgcttttgt gcatgagctt caccactgcc gacaccgagg aaatggaggc gatggtatga
     4441 aaaagggccg cctgcttgca aacgcatatc tggtgctggt gttcggcttt ttgtacgtgc
     4501 ccatcgccgt gctcatcgcg ttcagcttca acgaaagcaa aagccgcacc gtgttcacgg
     4561 gctttacctt taaatggtac cagaacctgt tccacaacga catggtgctg aaaagcctgg
     4621 gcgtttcgct gggcgtggcc tttgcggcgg cgtgcatcgc caccgtggtg ggcacgctgg
     4681 ccgccatcgg catcagcagc atgaataagc gcgcccgggg cgccgtcatg gccgtcagct
     4741 atgtgcccgt cgtcaacccg gagatcatca ccggcgtttc gctgatgctg ctgttcgtgg
     4801 tgttcaaccg gcaggtcagc gcggcactct cgtggcttct gcagcgcgag gtggattttc
     4861 agggcttcgg cctgggcacg ctgctcatcg cgcacatcac gttctgcctg ccctatgtca
     4921 tcttcaacgt cagccccaag ctgcgccagc tggacccggc cgtttacgac gccgcgctgg
     4981 accttggctg caatccccgg caggcgtttt tcaaggtggt gctgccgcag ctcacgccgg
     5041 ccatcctgtc ggcgtttctc atcagcctca cctattccat tgatgatttc atcatttcct
     5101 atttcacctc cggcaccatg cagacgctgc ccatcgccat ttacagcatg acgaagaaaa
     5161 aggtcagccc ggagatcaac gcgctttccg ccatcatgtt cgtggtgatt ttaaccatca
     5221 tcctcgcggc caacgccgtg gattccgcaa aagagcgccg cctgcgcagg gccgcgcgcg
     5281 cagaggaggg caggcgatga agcatatcct gaaggcaaaa aaacgctttt tgtgcctgct
     5341 tcttgcgggc gcgctcgcgg cctgcctgtc ccccgcggcc cttgccgcac cggacggcgt
     5401 ggaggttgcc cccggttacg attacacccg cttcaaggac gcgggcatta cgctgaatgt
     5461 ctacaactgg ggcgaataca tctcgggcgg cgccgacggc tccatggatg tggtgaagga
     5521 atttgaaaac ctgacgggca tccgcgtcaa ctacaccacc tacgacacca acgagagcct
     5581 gtacgccaag ctgaaaagcg gcggcggcaa ttacgacgtc atcatcccca gcgattatat
     5641 gatcggcaaa atggccgccg agggcatgct ggaggagctt gaccccgcca atatccccaa
     5701 cgcggcgctc atcggcgagg cataccgctc caaaccgttc gaccctcaga acaaatacag
     5761 cgtggcctac acctggggcc tcgtgtgcat cgtgtacaac cggacgatgg tggacgaggg
     5821 cgacctggaa cagggctggg gcatcctgtg ggacgaaaag tacgccgggc aggtgctgat
     5881 gttcaacaac agccgcgacg cgttcgccat tgcgggcaag ctgctgggca attccatcag
     5941 ccccggctcc gtgcaggaga tcgagcaggc ggccgaaaag ctgaaagagc agaaatccgt
     6001 ggtccagagc tatgtgatgg acgaggtgtt cgacaaaatg ggcggcggcg aggccgcgct
     6061 ggccccctat tacgtgggcg acggcgtgac gatgatggcc gacaaccccg accttgccat
     6121 gttcatcccc gccgagggca ccaatatcta catcgatgcc atgtgcatcc ccaaaggcag
     6181 ccggaacaaa gaggccgcgg aaatgttcat caacttcatg tgtgaaacgc aggtggcgct
     6241 ggccaatgcg gagtacatct gctattcctc gccgcaggta gaagtccccg cgcttctgga
     6301 cgaagagacc cgggagaacg agatgatgta ccccggcgca gacatgcttt cccggtgcga
     6361 gaccatggag gtgctgcccg acgagctgaa cagcgccatg gacaaggcat ggagcgatgt
     6421 gcgcagctac gacgccgcgg gcaacgggtg ggtgatgccg ctgatgctgg tggcaatgct
     6481 ggccctttcc ggcgtaggct actggcgcaa agcggtacgc cgccggcgca gccagtttta
     6541 aacgccgttc cctttttaca aggcaatgaa cgcaaaggcg ccgccgttct tcggaacggc
     6601 ggcgctcctg tatgtcgaaa aagactaagg caacgccgca caattcacgg ctgacgcctt
     6661 aagcg
//
LOCUS       DAWBSB010000122         6644 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_2233_length_6644_cov_2.910305, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000122 DAWBSB010000000
VERSION     DAWBSB010000122.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6644)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2233_length_6644_cov_2.910305"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1050..1059
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1325 a         1946 c         2100 g         1263 t
ORIGIN      
        1 atgacaacgg acctcggcca ggacagccag atacagacat acctgtccca caacaaagac
       61 gcatcccgcg cgttttccag cgaatacaac gcactgtatt atctggtgca ggattactat
      121 aagaaaaacc gtgtcaattg tgtttccaac atcacgctgt atacggggaa ttttacgctc
      181 cgctccaaca cgtccgccaa cgtgtatgtg ccggatgaag tttacaagga tttatatgag
      241 gcggcgctgg gggcgaatgg cgcaacggtg tgcgtcacgg attattcccg ggactacggc
      301 ctgttttttg tcaaagcgat ccgccgcatt gaaaaccttc gcctggatac gctgggcatc
      361 gaggtcgtgc aggtggatct ggaaaaactg gtgaagtcca gtgtgtcgct caaccagtac
      421 gacaacgtta tctttgtttt aacgcggccc caggatggcg cggtgatgta ttcttccgcg
      481 ggcgaagccg ggcctcaaac gctgcccacg ctggacacgg aaagccgagg ggatatctgc
      541 aagctggacg gcgtgaaata ttttgtcacg gcggacggca gcgcggatta cccgtgggat
      601 tatctctgtc tggttcccta caacgaaatt tatgcgtcca tccgcgacgc gctgcttttc
      661 tgcatcctgt ttttcctcgc gggcatcgcg ctcacctttc tggtttgcca ctttcttgtg
      721 gaccgtatta cgcgccgctt tgcaaagctt atcagccaaa tggaccagtt tgccaagtat
      781 acgcccgata tggtgctgcc ggtggacgag ttctccgggg aagagcggga ggcgggcgac
      841 gaggtcggca tgctcagccg tcatttcagc catatggcgc gggaaataga cggtctgatc
      901 cagacggact acaccaaccg catcctgctg cgggaggccc agctgaaggc gctggaagcg
      961 cagatcaatc cgcattttct gtacaatacg ctcagcgcga tcaactggcg ggccaagatg
     1021 cgcggcgcac aggagatatc ggatatggtn nnnnnnnnna catggtggag gccctgagca
     1081 accttttgcg cgttacactg aacgacgaca gcgggctgat cccattggag caggagctct
     1141 ctctggtgca gtcttatatc gtgatacagc gcatccgttt tgacgagacg ctggattaca
     1201 ggctggcggt ggaggcggac gagctgaaag acgttttgat tccaaagctg acgcttcagc
     1261 cgctcattga aaacgccatc cgctacggtc tggaacaatc cactcagatt tgccgcgtgc
     1321 agctgagcgt ccggcggcag ggcgcgcagc ttgtgctgct ggtgcagaac gacggttctc
     1381 tgtatgagga cgatttgctg gaaaagctgc gccagaaaac cgtgacgccc cacggctatg
     1441 ggatcgggct tttgaatatt gaaaaaaggc tggaattcgc ctttggaaaa gaagcgggac
     1501 tggagcttta caacgaagac ggaatggcca cggcgcgcat tacgatacct tgccgtgtga
     1561 gctgaaaaag cggtattgtg acggagggga gagcaaatgc tgaaactgct ggttgcagac
     1621 gacgaacgga tcttacggga ggcggtatgc tatctcatcg attggaaaag catcggcatc
     1681 gagctggcgg caagctggaa aaacggccgg gaggcttatg aagaggcctg caggataaag
     1741 ccggatatca ttctgaccga tatcaaaatg cccggccttt cggggctgga ccttgtggaa
     1801 aagctctcgg agccgggccg ggtgctggag ttcatcctgc tgacgggtta cgcggatttt
     1861 gagtacgcgc accgcgcgct ctcccaccgg gtgcaccact atctgctcaa gccgtgcaac
     1921 gaacagcaga tcgtcgaagc ggtacagcag gcggcacagt ccatctacac gcgccgccag
     1981 ctgcagcaaa tggagccggc ccgtgcgggt gtggcgcgcc ctgaatacag cgagacggtg
     2041 caaaaggtcg tggactatct ggaaaagcat tacagcgacg ccgcgctgtc gctcaaaagc
     2101 ctggcggcca attacctgtt tatgaacccg gattacgtga gccgccagtt cctgcagcag
     2161 acgggctacc gttttgtgga ttacctgctg gcgctgcgca tccgcaaagc gcagtggctt
     2221 ctggtgaacg gcgtgccgcc gcagcaggtt cccgagcggg tgggttacag cgcgaatcct
     2281 cagtattttg tccacctgtt ctccaaggcg acggggatga cgccgcggga atatgcgcag
     2341 gcgttgcgca ttgagccata aagaaaaaac aacacaaaca gctccccccg cacgaaggcg
     2401 aacgcccggt gcggggggag ctgtttgttg aagcgaagca gagtatatca gcagtctttg
     2461 caggcctgtg cgccgtattc ccgcagcatc cgggcatagg cgcgataagt ttccagcgtg
     2521 gcgccggggg gcgtttgatg gtccattgca ggcagatacc gcccgctgcg gatgacgggc
     2581 agaagacgct cgaattcccg gcgcacggcg tccggccctt cgaacatgca catcttgtca
     2641 aacccgccga tcatcaggaa atccggatat tcccggcgta tggcggccac gtccacgccg
     2701 gactggcgct ccagaggcag gataccctca acgccggctc cgcggaacca cggcaccgcc
     2761 cgtgtgacgt caccatcaga atccacgaac acatgggtgc cgtgtttttt gatctccgga
     2821 atcaggcgct ggtaatatgg cttgatgaac tcgttgaaaa gttcttcgga aagcatgggc
     2881 ccgtggttat agctcatatc ctcggcaatg gtcataaaat ccggcttcat ataacgggcc
     2941 agcgcgtcca cctgcgcgat ctgccattcc agcagatctt cgcagatgcg gtgatacagc
     3001 tccggctggt cataaaagga ataaaggtgg ttttcaatgc ccagcagtgt gcgcggccac
     3061 cagaaaaagc cgtccagagt ataccagaca agggtctggc cattctcata gaatggaagc
     3121 gtttgccgga tcttgggcag catctgctgc acagcattct ccggcaaaag atacgggcgc
     3181 agacgctcat agtccgattc attttcaaca atgcccctgc cgtgtgacgt ttcccgaggg
     3241 cagtccggtg ttttgtgggg cagccagaac tgggtgttgc ggtcaatgcc cacaaagtct
     3301 gccatagcgg cttcatccag cccgcggggc agtccctggc cgtaccaaag ctccagcgtc
     3361 ttatcccacc agtaggccca ttctatggcg ggcagccggt ctacggcggc gtcgccggcc
     3421 atgacacggc gaaagcgttc tcttgtggtc atggggtatc ctccctgtct gtataggtga
     3481 tgcgcagggt ctttatctca aaggggcgga attccaggtc tgccggacag ggggcaaacg
     3541 gctgctccaa catgtcgcag agccgtgcgc cggagcagcc cggagcgagg gagaggcggg
     3601 tgcgcgcgta agagccctcg cactcataca ggcgcaggat gaatgcgtgt ccgccgtcct
     3661 cgcagggctt gacggtctcg ggcatcacat tggccgcgtc tacacggaca agcggctccg
     3721 aaggcaggcc ctcgcctgag gcgggcagcg ccgcgtactg gaaagcatag gcgggccgga
     3781 cgacactgtg cgcgcagaag cctgcggtgt gcggcaaaaa tgcataccgg aaggtataaa
     3841 cgccttcgtc gccgcgcggg tccggccgca tgccgccttt ggccagcgag agggcgatcc
     3901 ggccgccctc gacgctgacg ccatacttgc agtcattcaa aagcgcgacg ccgtaggagg
     3961 gctcgctgag atcggcatat ttgtggcagc acacctcgaa tttggcctgc tccagcgaat
     4021 cgttgcgggt ggtggggcgc tggatgcagc cgaactggat ctcctgccgg gcggcgggag
     4081 cctgtacggt ggtgtcgaac gcggctttca aaaggcggtg ctttccgtgc cattccagct
     4141 cggtgtcgaa ttccacaagg ggactgtcgg cgtggaagat gatgtcctgc cgcacaacgg
     4201 aatcgccgca cacggtccag gtgcagcgca cgcggtattc cacaggcccg tcggccgcga
     4261 ccacccggct ttgcagcacg ccgtcgggcc gcagcttttc catgcagtcc gcgtcgacgt
     4321 cccatccgtc ccacgcggcg gggacatcct cggcggaaag gaacgtgtta aggggcagcc
     4381 ccgcgcacag ctcgcggccg gtgcgccggt cgacaaagga ggaaatggcg ccgccggggg
     4441 aaaaacgcac ccgcgcaaag ggtgtttcca gccggtcgcc gcaggcatgg aagggcgagg
     4501 gtgcttcatc cgcacccgcg cacagtgaga gcggcgtgaa cgagaacggc tgctggcgca
     4561 ggccggctac ggccagaaca cgcgcgccgc cgcgcagctc cacgagctgc gtgcgcaggc
     4621 cgggcgcatg catggccgta tttacgggca gataaacggt atccgtccgc gcaaaaccca
     4681 gcggattgag cacgccgccc atctgctccg gccgaagcgt tgcatccagc cgacgcccgg
     4741 cctcttccag cagagcggtg gtctggcgca gggattcgtc gtgcgcctcc ggaatgcagc
     4801 tgccgggcag gatgtcatgg aactggttca ccagcagggt ctcccacagc ggccggcagg
     4861 cggcgccgca cgcgggcgcg ccgctgtgca cggctgccag caccgtgacc agctcaaggt
     4921 cgtgcagggc ggtttcggcc atgcggttgt tctttttgat ctgctgtttg cccgtcagcg
     4981 ttccacggtg cagctccaga taaagctcgc cccggtattc ggggaattcg cggccctcgg
     5041 cctccaggct ctgcataaac tccgacgcgc tggagtagcg taccctgggg cagccctcca
     5101 ggtccgcaag gcggcgcgct gtttcgatca tctcaaactg ggggccgccg ccgccgtctc
     5161 cgtacccgaa ggcgatgagc ctctggtggg agatctgttt gttgtgcagg cagtcccggt
     5221 agccgacgcc gttgacacgc tccagcagcc cctttgggtc gggccaggta tccatgacaa
     5281 agaagtgggc cagaacgtcc gtgccgtcga tgcctctcca ggtgaaggtc tcatagggga
     5341 aggtgttcgt gtcgttccag gccagcttgg tcgtgagaaa atattttacg ccgaagcccc
     5401 gcatgatctg cgggatggcg gcggaatagc cgaaggtgtc gggaagccag aagcagtccg
     5461 acaatacgcc gaacgtatcg cgtgtgtagc gctggcccca cagaaactgg cggatcagcg
     5521 cctcgccgcc caccagattg cagtcgcatt ccacccacac cgcgccgttc atctcgtatc
     5581 gcccctgcgc caccgcctcg cgcatgcgct caaacagggc gggatattcc cgctgcatcc
     5641 aggcggtgtg gcaggaggcg ctttgcagaa agcggtattc ggggtactgc tccatcagac
     5701 tgagctggtt tgcgatggtg cgcgcatttt tgcgcacggt ctcagcagtt ggccaaagcc
     5761 aggcggtgtc catgtgggaa tgccccagca gagccgcgcg gggtgcgctg tcgccgttgc
     5821 ggcaggccag cgcgggggcc atgacggcgc gcgccgcgcg caggctctcc agccaggtct
     5881 cacggtccac ggcctgtggg gagaggtaca gcgtccggtg cacctgcgcc agggtattga
     5941 gcacgccggc gcggcggaag gagttttcgt ccagcatctc ggcaagctgc agcagcacgc
     6001 gcaggtcaaa caggaaatcg gccacaagct ggttcttggt gcagatcagg atatcctgtg
     6061 cctggaagga gaaggcctca gcgtcctctc cgcccagagg gccgtccggc tcaaagggcg
     6121 cgcggccggg cacggggtgg cccgcataga attccagcgc gacatccatg ctgcgggcgg
     6181 ggtccgcctg ggcgcacagc atgtcgcagt agtgattgcc gtggcgcgtt acgacgattt
     6241 tggtggcaaa ggtgcccttc ggcatgccgt ctacccagag catggcctca tagccgccca
     6301 cgcggggcat cagatagagc gcccgccctg cgagctgttc ggagggctga taccggcctt
     6361 tgaaccagca ggtctgccac ggaccgcccc aatgggggac cggcgcgtgg aagcattcgt
     6421 cgggcggcac cgcgcgcaga tgctcccggg tctcgaacat gcgatagtcc agcttgccct
     6481 ggggctcgaa caggtacggc tcgaatacct ccaggcaatg ggacagcttt tgaagcagct
     6541 ttttattctg ctgcgggttc agcatatgcc gtttcctcct tatccgcctg tactcagtac
     6601 cggcccagcc tgtgggcggt ccgatacatg gcaatcacgt tttc
//
LOCUS       DAWBSB010000123         6621 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_2238_length_6621_cov_3.349223, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000123 DAWBSB010000000
VERSION     DAWBSB010000123.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 6621)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6621)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6621
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2238_length_6621_cov_3.349223"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4249..4258
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1343 a         2054 c         1816 g         1398 t
ORIGIN      
        1 ctatttcatg gacaccaatg tgttcgacta catcaacatt gataaaaaga acacctttgt
       61 agccagcggc atgggcgatt tcgaggccaa tgcccgcgag ctggaggaaa aggtgcgtga
      121 gggcggcgcg gcggatctgc agctgctggg catcggcaac aacggccaca tcgcattcaa
      181 cgaggccagc gaccacctca tcgccgtggc ccacaccgaa aagctgaccg agagcaccat
      241 caacgccaac gcccgcttct ttgagaaaaa agaggacgtg cccaccatgg ccatcaccat
      301 gggcatgggc gatattctcg ccgcgaaaaa ggtcgttctg gccgctacgg gccttgccaa
      361 ggtcccggcc atcaaggggc tgatcatgga cgacgtcatc accacgcaaa acccctccac
      421 gatgctgaag atgcacgagg acgccgtcgt cgtcatcgac agggagctgg ccgacgccgt
      481 gggctacaga gcctgacgcc aacagctgaa aaacgccgtt gcagcggccc gccgccggga
      541 aaccggagcg ggccgctgtg tttttcacag cagaaaaacc gccctgccgc cgctgcgttc
      601 gccgcgcgaa aacgcgtggc ctcttactct ctccgcaaat atcttgttcc gcctcttttg
      661 aggcctgtcg caaaggcgca ctcaagattg acttttatct ctattccgga taaagttaag
      721 gtaataaacg tccaatgagg tgtaatatgt ttggccttta catccatatc ccctattgcc
      781 gttccaaatg ccgttactgc gacttctatt ccaccccatg cccggacggt gtacccgagg
      841 cgtacattga cgccctgtgc cgggaaatgg accgtttttc gccttgcggc tccacgccgc
      901 tgcgccccga cacgctctat ttcggcggcg gcacgccctc gctgctgacg cccgcgcagg
      961 cggcccgtct catccgcgcc gccgggccgg cgcccggtgc ggagatcacg ctcgaagcca
     1021 accccgagac cgtcacgccc gagcgattgg ccgcgttccg ccgggccggt gtgaaccgcc
     1081 tttcgctggg catacagacg gcgcgggacg acagcctggc ccgcctgggc cgccgccata
     1141 ccgcggcgca aagccgccgc gcgctgggaa tggccgtgca ggccgggttc accgacatca
     1201 gcggcgacct gatgcttgcg ctgcccgctt ataccatggc cgagctggac gaaaccatcg
     1261 ccctgctggc ggagagcggc tgcacccata tcagcggcta tctgctgaag atcgagccga
     1321 acacggtgtt cggcaagcgc ccgccggaag cactgcccga cgacgacgcg gctgcggatt
     1381 tttatcttgc cgccgtggaa aagctggcgg cgctgggcta cgcgcaatac gaaatttcca
     1441 atttcgcccg ccccggcttt gaaagccgcc acaaccggat ctattgggac tgcggcgatt
     1501 atctcggcct tggccccgcg gcgcacagct gcatgggcgg caagcgcttt gccacgccgg
     1561 cgggcacggc cgcgttcctt gccgcgcccg cggtgtacga accccagggc gaatgcaccg
     1621 ccgaagatta catcatgctc cagctccgtc tttcccgcgg gctttcattg agcgcgctgc
     1681 gcgcgcagta cggcgtatcc ttctcctccg aaaagctccg gttcctgcaa acgcttgcgg
     1741 agcatgggct ggctgttttc gacggcagcg tcctgcgcct gacgccccgg ggaatgctgg
     1801 ttcagaacag catcctgtgc gagcttgtgt aaagcttttt tactgtccgg tacactttct
     1861 ttgcatttca aaaaaacacc cggcgcaggc tctgtgccgg agtgcagggc gttcttctct
     1921 ccaggtgtcc tatgatttaa cttttttata actttgcaca gcaaagctat aaaaagctca
     1981 ttttaatggc cgcaggccgg ggacggcccg catggccaaa aaacagcctg cgactatttt
     2041 ttatttattg ccgcgcagaa ggctttttca atactccacg ccctcccggg ccgcggtgcg
     2101 cgcgccgtcg gcaaagggat gcttgcgcgc ctcgatgacg ctcacataat ccgccagagc
     2161 aatcagctcc tccggcgcac cgcgcccggt gagcaccagc tccgtgttcg gcgctttctc
     2221 ccgcactgtg cgcagcacat ccgccagaga cagcatcccg gcgttcaccg cgcacagcac
     2281 ctcgtccagt accacaaggt catactccgc catgtgtgcc gccgcacggg caagcgtgtc
     2341 cgcctggcgg gcgcggcatt ccgctttttc ctcttctgtc atcgtccagg tgaatttttc
     2401 ccgttccacc gggcagtaaa actccacgcc aagcgcagcc aggctctttg cctcgccggt
     2461 ttttgcaccc tttaaaaatt catagaacgc ggctttttta ccgtggcccg cggcccgggt
     2521 gcaaaggccc acggcagccg tcgtcttgcc ttttccgtcc ccggtataga tgtgtaccat
     2581 gcccatcatt ttccctcctc tccatccaga tatccaaacg ggatgatatc gctcacttcc
     2641 acgatgccgt cgcgcatcca cagctccggg ctgcactgaa gcagataacc gcagccgctg
     2701 tccgccatcc aaagctccgg cgcgcggcgc ggcatgccgc gctgggccag catgctcatg
     2761 atgacgccgc cgtgggtgac gcacgccgct tcggagatat tgcttttcat cataaattca
     2821 aacatgccca tcagcacatc cgaacagcgc ttgtggaact ccgccgcagg ctccccgccc
     2881 tcgggcgtat agtgctgcgc cgggtccatc cactgcttga aatgcgcatc ctgcaccagc
     2941 tccgtcacct tacggccctc aaattcgcca aaggcgtttt cccgcaggtc cgccaggatg
     3001 atcttatcct ttgcgtccgg gaacagcagc tccacagtct ccaccgcgcg gcgcataggg
     3061 cttgaaaaca ccgtttctgc tttaggatac ggaaagcgtg cggccaacgc gcgcagctgc
     3121 gccgcgccct gtgcgcacag gggctggtcc gtcccgctgc ccatataaat gccgtccagg
     3181 ttgccctgcg tgacgccgtg gcgcacaaga tacaggcggt aatttttcat aaaagttcct
     3241 cctatcggtc cgctgcggga atttcagcgg ttttaattat ttttcagcca catttttggc
     3301 caaaatctcc agcccgccca ctatatttag gcacatgggc ggcgttgggc cgaagatata
     3361 gcaaaccctg tcggacccgg ccgggccgtg gggttttttc ggaaattttt gtatttacaa
     3421 aatgtttttg ctgtactata atttacagta cgtaattgtg taaggggtat gcggtaatgg
     3481 tatcggattt cagcaggatc atcacgcttc tgcgcaaaga aaagggcgtg acgcaaaagc
     3541 aggccgccga agatctgggc gtttcccagg cactgctgtc gcactacgaa aaaggcatcc
     3601 gcgaatgcgg gcttgaattt gtggtgcgcg tggctgatta ttacggcgtt tcctgcgatt
     3661 acctgctggg ccgcagcgcg gaccgaagcg gtctcaccct ttcggtggag gatatcccca
     3721 gccccgaagg cgcaaaggac ggcgtgttcc gcggcagcgt gctgcccact ttcaacaaaa
     3781 agctcatctc caacagcctg aatatcctgt acgacaagct gaacgcctgc ccggacaagg
     3841 gccttgtggg cgagatttcc ggttatctga tgctggccgt gtataaaatg ttccgtctgg
     3901 tatatggcgc gggaccgaaa aacaccagca gcatgttcgg cgtgaagaaa aatctgtacg
     3961 gcggctattc cgacgccgcg atgcacattg cggaatccaa cgttaaggcc attctcgcgg
     4021 gcgaggacat cggcctgggc gcgccgctga aggacaccag cgcctttgcc atgacgacag
     4081 aaagcctttc gcgggattat cctctgtatg ccaccagcct gctgaacctc atcaagacga
     4141 gcgaggcccg catcgaaaag gaaaaataag cttttttatc atagcatgaa ttttcacaaa
     4201 aggaaagccg ccactttgtg gcggcttttt acgcgctgct ggctacacnn nnnnnnnnaa
     4261 acagccggaa tgcgttggac gcagctccgg cagcggggac gccccgcctt ttatttaagg
     4321 gccgcgatcg cctcgcggca tgcgccgcag atgttccggc cctgatactg cgcgacgcct
     4381 tcggcgctgc cgcagaagat gcacgcaggc tggtactttt tgagcacaat gctgtcattg
     4441 tccacatata cttccagcgc ggcgtccttg tcgatatcca gtacccggcg cagctcgatc
     4501 ggcagcacaa tacggcccag attatccacc ggacgtacga ttcccgtaga tttcaattct
     4561 cccacttcct ttttccggcg ggtatccctc ccgccggata tcattgatat tactatacag
     4621 aattttacag aatctgtcaa tatgtggaat aaaaagaata ttttaacaat ttgtaaactg
     4681 atatacgcta aaatacggtt aaatttgcaa aaaaccgact tttttgtaga cttttgtcac
     4741 tgtgcggtcc gcaatggcgg cagcacgccg cgcgcccccg gtgagcacgc cgtccagata
     4801 agccttgtcc gccaggatgc gtttgtattc ggcctggatg ggggcgaaag catccttcac
     4861 cacctcggcc acggccagct tgaactcgcc gtagcccctg cccgcgaact ccttctcaat
     4921 ggcggcataa tcgcggcccg tgaacacact gtaaatgctc atcagatttg tgacgccggg
     4981 cttgtccccg gcggcgcgca cgcatccgtc ggagtccgtt acggcgcgct tgaatttgcg
     5041 cacgatggta tccgcgtcgt ccagcatcag cacaaagccg ttgacgttgg tgtcgctttt
     5101 gctcattttc ttctccggct cctgcaggct catgattttg gctcctgttt tggggatgta
     5161 gccgtcgggc accacgaagg tgtcgccgta tacgccgttg aacctctggg cgacattgcg
     5221 cgccagctcc agatgctgct tctggtcgat gcccacgggt acgaggtcgg cattgtagat
     5281 gaggatgtca gccgccatga gcacaggata ggtaaacagg cccgcgttga tatcctccgg
     5341 atgcctggca ctcttttcct taaactggtg catacgcgtc agctcgccga agggcgtgct
     5401 gcaggagagc acccacgaca gctccgcatg agcgggcaca tggctctgta cgaacagcac
     5461 gctcctgtcc gggtcgatgc cgcaagccag cagcagcgcc gcagcctccc gcgtctggcg
     5521 gcgcaggtcc gccggcgttt ggcgcacggt gatggcgtgc atgttggcga tcatataaat
     5581 acagttataa tcgctttgca gcgcgtccca gttgcgcacc gcgcccacat aattgcccag
     5641 cgtaaaagtg ccggtgggct ggatgccgct caggatgcgt tttttttgtt cttccatttc
     5701 gggttccccc tgctttttta caatatataa attattgtag cacaccctaa gcctgaaagc
     5761 aatcgaggcc gccggcaaaa tcgccaacct ctgcgggacg ccaaacgctt tgtgctgcgc
     5821 ggccgctttt tgggccccct ccgttctcct ttccggcgcg caaaagggca attttgcgct
     5881 gtaggggtgt tttcccggcg cgtgtccggg ctgcaaacgt ttttttaaca ggccgggcaa
     5941 ttgacaaaca gcctgcttgc ggctaaactt aataaggaat aaaagcgcgg cggaacgccg
     6001 ttttttcatg tccgtccggc tgcgccgggc aaaatccaaa aattgaggag taccaaagct
     6061 atggcatcga atatcgtccg tacccgtttc gcccccagcc ccacgggcta catgcacgta
     6121 ggcaacctgc gcaccgccct ctatgcgttt ttaaaagcca aaagcgccgg cggaacgttt
     6181 attctgcgca tcgaagacac cgaccaggag cgttatgtgg agggtgcggt ggacatcatc
     6241 tacaataccc tgcgcgaaac gggcctcatc tgggacgagg ggccggacat cggcggcccc
     6301 gtcggcccct atgtgcagag tgaacgcatg ggcatgttca agggctatgc cgagcagctt
     6361 gtgaaagaag gcaaggctta ttactgcttc tgcgacaagg agcgtctgga tgaaatgcgc
     6421 ctggtgcaga aagccagcgg ccaggctccc cactacgacg gccactgccg cagcctttcg
     6481 ccccaggagg tgcaggcaaa gctggacgcc ggccttccct acgtcatccg ccagaaaatg
     6541 cccaaaaccg gcaccaccag ctttgaggac gtggtgtacg gccacatcga ggtggacaac
     6601 gccacgctgg atgaccagat c
//
LOCUS       DAWBSB010000124         6618 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_2239_length_6618_cov_3.499771, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000124 DAWBSB010000000
VERSION     DAWBSB010000124.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 6618)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6618)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6618
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2239_length_6618_cov_3.499771"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1248 a         1981 c         2109 g         1280 t
ORIGIN      
        1 atgcaaaggc cacgacgcag gaagagatca tgacgcttgc cgccaaatac gcataaggag
       61 gaatcactgt gggaacgaat gccaaactga ccatcaagca gcgcttggaa aacttcaaag
      121 ccatggaaaa ggaggataag cggcgctggt ggggcgagtt tttcattgat aattcgctct
      181 atatcttcct tattgcggct atcatcggca ttgccgtcta taacccgaac ttcgtgtcga
      241 tgaaatccat catcaacatc gtttcgctct cggccgcgaa tctgcccatt gcgctgggca
      301 tcgcgggcgc gatcattctg acaggcaccg acctttcggc cgggcgcgtt gtgggcctga
      361 cggcttgcgt ggcggcttcg ctgctgcaga tggcggagta ccccaataag atgttcccgg
      421 gcatcggcac tgtccccgtg cccatcgtca tcttggcggt cattgccatc ggcgcgctgg
      481 tgggcgctgt caacggcttt tgtacggcca agttcaatct gcacccgttc attgtcacgc
      541 tggccaccca gctcatcgtg tacggcacgc tgctcattta cctgatgcag ggcaacaaca
      601 acgggcagtc tatctccggc ctggataaaa gttataccaa tctcatcacc gggagcatcc
      661 tttcggtgcc ggacggcaag ggcaatatgg tccccatccc caactttgtg tggtatgcca
      721 tcgtgatcac cgtcgtgatg tggttcgtct ggaacaagac gactttcggc aagaacatgt
      781 tcgccgtagg ctccaatccc gaggctgcga acgtctccgg cgtgaacgtc gctgccacca
      841 ccattctggt gttcacactg gcgggcgtga tgtacggctt caccggcttc atcgagggcg
      901 cgcgcatcgg cgcgaatacg gcaaacaccg gcttgaacta tgagctggac gccatcgcgg
      961 cctgcgtcat cggcggcgtc tcgttcgtcg gcggcatcgg caagattcgc ggcgtcatca
     1021 tcggcgtggt gctgctgcgc attatcttcg tgggcctcac gtttatgagt gtctcgtcca
     1081 atctgcagta catcatcaag ggcctgatca tccttgtggc ctgcgccatc gatatgcgca
     1141 agtatctggt acgcaagtga ccggagggcg ccatggaaga agaacaccgc tcggaaggcg
     1201 cgctccggga cactgcgccc gagccgcggt accgcggctc gaacaatatg taccgcggcg
     1261 tgacgatatc cgtgaaggcg ctgaccgggg cgattttggc cggtctggcc gcgcttgcgc
     1321 tcgccatcgt gtttcttgcg ggcagcggcg gattcaccgt tacgtttgaa accgacggcg
     1381 gttcccaggt gcccgcgcag acctgccgct acggcgagct ggtccggcgt cccgaggacc
     1441 ccgtgaaaga gggatatgtc tttgacggct ggtattggga tgtggaacgt gagcacagac
     1501 cctgggattt tgcgcaggat acggtggagg acagcatgta cctctacgcg cgctggcagc
     1561 ccgtgccgta aatgcgtcgc cgcgccggat caaagcttaa agggccctgt acggttttcc
     1621 gtacagggcc gccgtgtttt tccgtcctta tgctttcctt agaattgcgc gttaggatac
     1681 agctgcggaa gcaaaaaaga gtggagaaaa catgaaaaaa caaaatggaa tacgcgccgt
     1741 cctgtggacg gtcctcatgc tggcggccgt tctgacgggc tgctgtgccg atggagagga
     1801 cgcctgcacg ctggacccgg cgaacccggc acgctttacc tgcggcgggg tggaatatat
     1861 tacgctgcgg gaggctgtac cgcgtgatgc agtggggcgc aagctgggcg atatccagac
     1921 actggcggcg gtggacgggg aagggcgcgt cgtggatacc tgcggtgtga cgctttccct
     1981 ctctgctttg gcccggctgg aggaaaggct gccggagggc gggcgcaccg tgcaggtgct
     2041 gaacgtatgg gcgctgcccg gcacgacgga cggcgctgtg gccgtgatgg tggacggcac
     2101 attccgccgc gcagtacccg cggatgtact gccgccggaa gcggaggtcc tcagctttgc
     2161 acggcaggcc gggggatacg gggaggcctt tgcggtgatg ccggaggact gccgcaggct
     2221 cacttgcgga gagacagtct accttgtcac agatacaccc gttgcgccgg aggcgcaggg
     2281 cgcatatctg ggcgtgcttg cccgctcctg cgtgttcgat gcggataccg ggcgggaact
     2341 gacgcgcgcg gagctggagc atatcgaacc ggtgccgggg ccgctttccg cccagcggcg
     2401 ggagagccgc gtgtacggcg cggtgtacgc gctggaggga acggacagcg gatcggctgt
     2461 ggcggtagag gtggacggcg cattgctgcg ggccgaagcg gagcggcgcg tgccgggcgc
     2521 gtcctgacgc tgccatccgg gcggcccttt gccgcatccc tgccggaggg ctggcgcaaa
     2581 aaaaggcgct ctcaacagcc ggaatggctg cggagcgcct ttttttgttt ttccgtgggc
     2641 ctatttccgt acaggcgcag gcccggtgct ctcccggacg atcagctgcg gacgcagcag
     2701 caccttgctt acggcaacgc tgttcatcag actgtccagc aggctgaacg cacttttgcc
     2761 cagcgccagc ctgtcctgtc ggatggtggt aagaggcggt tcgcagccgg cggccagcgg
     2821 aaggtcgtcg tagcccatca cgctcacgtc cccgggcacg cgcaggccca gacgccgcag
     2881 ctcggccagc accccccggg cgatgccgtc gctggcgcac aggatggctg tcgcgccctg
     2941 tgcaagaaaa tgcggcacgt attcgcgcgc acagttttgt tcataatacc cataggccat
     3001 catccgttcg tcggcggaaa gcccgcacag ccaggtggcg tgcaggaaag ccagccgcct
     3061 ttcctcagac accattgaat cccgcgtgcc gttcaaaaag gcgattttcc ggtgtcccaa
     3121 tccggccaga tgccgcacgc cgagctccac gccctcccaa ctgtcggtgc ccacatatcc
     3181 gacgctggcg ttgggaacgc agttgtccag cagcactgtg ggcgtgcccg tgccggccag
     3241 cgcacgcatg tatgcatcct gcggcgtcag gccgagaatg aaggcgccgc cgtatccgcc
     3301 ctccagcagc aggctgccgt atttggtgcg cgcctgcgcc tcgggcgtgg tgggcagcac
     3361 atccacggcc catccccgtg ccgcggccgc cagccggaac ccggccacga tctcgtaccc
     3421 gaactggccg atgttggcgt attccatatt ttccaccagc acacacacct tgggctgtgc
     3481 ctgcctgcgg gtacgcttgg ccgcatagcc catttccacc gccgtgtcca gcacaagctg
     3541 gcgggtctct gcgctcacgt ccggcgcgcc gttcaatcct tttgaaaccg tgctcaccgc
     3601 gattcccagc cgttgggcaa tgtcccggat cgtcaccaag cgctgcgcct cccttcgttg
     3661 tccgatatcc tgtttagtat aacccgcacc gccgctcttt gcaatgggag agaaagaata
     3721 ttttattgaa atatcacaaa atacgccttg acgaaatccg tatccaatgc taacatggtg
     3781 gtgttctacg aaaatttgcg aaaataaatt gcttttatcc cgcggcggcg gttcaggaaa
     3841 ggagtttttc catgaaaaga gatattcaga tactgacggg gcaggtgctt gcggccgact
     3901 gccggaaaac cattgcccag gctacgcccc gtgagctgta caacgcggtg tccaaagcag
     3961 cgctggacct cgctgcggac gcatggaagc gcgcgccggg caagcgcgtg gcgtaccttt
     4021 cggcggagtt tcttgtggga cgcctcgttt acgccaatct gctgaacatg ggccgcctgg
     4081 gcgaggtgca gcagatgctg ctggacgcgg gtgtggatgc gaacgtgttt gaagagatcg
     4141 aggacgcggc gctgggcaac ggcggcctgg gccgcctggc cgcgtgcttt ttggattccg
     4201 ccgccacgca gggcgtgccg ctggacggct acggcatccg gtaccagtac ggcctgttca
     4261 ggcagtattt tgaggatggc ttccagaaag agaccgccga tgactggcag cgctttggag
     4321 atccatggag cgttcgccgg gaggaggatg ccgtcactgt gcagttcggc gggcaggccg
     4381 tgcgcgccgt gccttacgac acgcctgtca tcggctacgg aatgaaaacc gtcaatacac
     4441 tgcgtctgtg gcaggccgag ccgttccagg cattcgattt caatttgttc aacgcccaga
     4501 aatatgccgc tgccgtgcgc gagcgggacg ccgccgaaga tatcagccgt gtgctgtacc
     4561 ccaacgacga caccgacgca ggcaagcgcc tgcgcctcaa gcagcagtat ttcttctcga
     4621 gcgcgtcgct gcaggacatg gtgcgtgctt accgcgcggc ccatggcggg gatttcaccc
     4681 attttgccga cgcatacgcg gtacagctca acgataccca ccccacagtg gccattcccg
     4741 agctgctgcg cctgttggtg gaagaggcgg gcatgcgttt tgccgacgca gtacaggtgg
     4801 cccacgatac ctttgcctac accaaccaca ccattatgcc cgaggcgctg gaaaaatggg
     4861 atcagaagct gttccgcggc gtgctgcccc gggtgtatcc gtatgtgaaa aagctggacg
     4921 cgctgctgcg ccgcacgctg gagcagaggg gcgtgcccat gggcgaggtg tcccgctatg
     4981 ccatcctgga ggacggcatg gtgcatatgg cgcgtatggc catttttgcc acccactcca
     5041 ccaacggtgt ggcgcgcctg cacactgaga tattgaagga caccgctctg cacgaatggt
     5101 acgagctgta tcccgagcgg ttcaacaaca agaccaacgg cgttacccaa cggcgctggc
     5161 tggcgctggc aaacccggaa ctggcggcgc tgctgcacga cgctgttggc gacggctggc
     5221 tgacagacct ctcccagctc aagcggctgg agccctgcgc ggacgaccct gcatttctgg
     5281 cgcgtttcat ggacgtcaag cgggagaaaa aacggcagct cgcggcgtat gtggaaaagc
     5341 acgagggcgt gcggcttcgc gcagacttcc tgctggatgt gcaggtaaag cgcctgcatg
     5401 agtataagcg ccagcttctg aacgcttttt ccattctgga cacatactac ggcctgaagg
     5461 aggggcgcat ctcccgcgcg gatttcgcgc ccaccgtcta cctgttcgga gcaaaagccg
     5521 cgccgggcta tgtgcgggcc aagggcatca tcaagtatat caacgagctg gcggaactgg
     5581 tgaacggcga tgcggacgtg aacggcctga tgcaggtggt gtttgtccag aactacaacg
     5641 tcagctatgc ggaaaagatt atcccggcgg cggatgtgtc cgaacagatc tccacggccg
     5701 gcacggaggc gtccggcacc agcaacatga agttcatgct gaacggcgcg gtgacgctgg
     5761 gcacactgga cggcgcaaac atcgagatcg cagagcaggc cggcgcggag aacgaatata
     5821 tcttcggcgc ccgcgccgat gagatcgcgc gtctgcagaa ggacggctac gacccgaacg
     5881 ccgtcctcgc ggcggagccg cgcgcaaagc gcgtggtgga aagcctggtg gacggcacat
     5941 tttccgacgg cggcacaggc atgttccgcg aactgtatgc ctccctcacc gaaggcgcaa
     6001 gctggcacaa gcccgaccac tacttcctga tgcgggattt tatcgagtac tgcgacgcgc
     6061 gcctggctgt caaccgggac tatcgcgacg cgcgggcttt cgcggcaaag tgtgtgcgca
     6121 atgtagcaaa cgcgggcgtg ttttcgtccg accgtacgat tctggaatat gcgcgggaca
     6181 tctggcacgt gtaaacgcat gggataaagc aaaacggcat ggagcaaaag ttccatgccg
     6241 tttttttatg ccttcagcat ccgccagagc gtggtgcggc tgatgccaag gctctgcgcc
     6301 gcacggcttt ggttgtcgcc gcttttttcc agtaccattc ggacgatctc ccgctcgatg
     6361 agggccagcg gctgggagag gtcgagagag gcggcggggg aggccgccgt atccgcgtcg
     6421 gcgggcgcaa gggaaagctc cgcctgcagg gcggcctgaa cgtccgacag ccggatttgt
     6481 gcatccgtgc acagttccgt cagccgttcc atcacgcggt ggaactgcag atagttctgc
     6541 gtccacggat actggcgcag ccgcgtaagc gcatccgcgt cggtgccgcg ccactgcctg
     6601 ccggtgtcta tcgtatgg
//
LOCUS       DAWBSB010000125         6551 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_2253_length_6551_cov_2.647783, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000125 DAWBSB010000000
VERSION     DAWBSB010000125.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 6551)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6551)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6551
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2253_length_6551_cov_2.647783"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5634..5673
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6249..6258
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1461 a         1907 c         1906 g         1227 t
ORIGIN      
        1 cgctgtcgtc cacggtctgg gcagcgccgc tcttgttgga gcccttcagc atatactcga
       61 tgttgccctg aaaataggtc agatcaaaca gatttttcca tgtcttggaa aggtcctttt
      121 tttcatactc tgtgggaacg cccagctttt ccgtcaaaaa ttcagcatac agcgatggat
      181 cgttggagcc gttggcgtcc acatctgtat tgaacagcca cgggtccagc ccgatgatga
      241 cgtttttcgg cagcttgccc gccttgtcat acaaagaaaa tacgctcagc acgtcgttga
      301 aatccccgcc ggaggtaccg ctgttgtaaa agctggtgcc gaccatttca cgcgtcaact
      361 gcaaaatgcg gctggaaccg agggcgatgg tctcaggggt ggtctcttcg ttcatgttgg
      421 cgacaatgac ttcaagcact tcgagctcac gtccattgag ctggttgaac ccgacaacgt
      481 tttttccttc aaacagcatc gtggcgatct cccgaagata ggtatcgccc aagaagtagc
      541 cctgacaatc cacgccgtaa ttgactgcga ccagcaacgc aaacacaggt gtaaacagca
      601 gtgcccccaa cgccaggcgc agcgcggaaa gcaaacggct gcccaaaaag cgcaatcgtt
      661 ttttcatctg ccgtcctcct taaaaccttc cgtaaataga gcccgagttg gcaaatgagc
      721 cgaagaacag catgcccagc accagcgcgt aatacagcgg ccaccgcagg aagatgggta
      781 ccttgcggat ggtgcggttc accggctcct gcacagattc cagcgtttcc accacaagga
      841 tgctcacagc cagtatcagc gccgtctgca ccgaaagccc gatggaaaca agcgcgcggc
      901 caatggcgtc cggcgcggtg aacagcttgc cccagcccga aaacagatgg ccgtagatat
      961 acagcgcgtc cgcaatgccc ttactgcctt caaacacctc cgacgaacgg aagaagatga
     1021 tacagcttgt gaaaagcaga taggtaacag cggtctggaa aatgcgccgc acaggcgtga
     1081 aatgatacag cggattatgc cgcgtcaatt tacgccgcgc gtcctgcgtg gccttgccca
     1141 cgcacaggta aacgccgttg agaaggcccc acaccaccat agacagggag gcaccgtgcc
     1201 acagaccgct tacaaggaaa acaacaagct ggtttatgta ctgccgcgcc ttgccacggc
     1261 ggttgccgcc caaaggaatg tacaggtaat cacggaacca gcttgtcagg ctgatgtgcc
     1321 agcgccgcca cagttcggta aaggagcggg cgtgcagcgg ctggcgaaag ttttccgtca
     1381 attcaaaccc aaacacggcc cccgcgccaa gcgccacgtc acatccggcg gaaaaatcac
     1441 agtaaagctg ataagtatac agcaagctgg ccagcagcag gatggggccg gtgtatgcgc
     1501 cgtaacccgg gttgccgtac accgcatcca ccgctgttcc cagcgtgttg gcaatgacga
     1561 attttttgaa aaagccccac agaatgcgga acagcccgcc tgaaacgcgg gcgtaatcga
     1621 aggtctgcgg cgttttgaat tgggggatca tatgctccgc gcgctcgatg gggcctgtga
     1681 cgatacaggg gaaaaagctg acaaagagcg cataaaagaa aaagttcttc tccgcacgct
     1741 gccggccgcg gtacacgtcg atgacatagc ccagcgcggc aaaggtgaaa taactgatgc
     1801 ccaccggcgc gagaatatcc aggctggggg ccgtataatg caggccgaag gaatccagca
     1861 gcgcagccag cgtttcgccc aaaaagttga agtatttata atagaacaga cagcccacgc
     1921 acagcgccag cgacacgacc agacagacgc ggcgcatcca tttgacgcgc agacgttcca
     1981 gcagcagcgc cgccgcccag gtgacggccg aggccgaaag cagcagcgcc acaaagcccg
     2041 cattttccgg gtcgtacata taaaacaggt aactgcacac cagcagccag tagggacgca
     2101 ccgccttggg cagcacaaag taggccaggc acgtgcccgc caggaacaca aggaacgcca
     2161 cagcgaaaaa gctgctcatg cgcaaaactc cttccgggga ggatcgccgc ccatgggcgg
     2221 cgccagacta ttgaaaaagg caaagaagta aaagaagcgt ccggggaaaa agctggcggt
     2281 ggcaaagtca tgcatctttg cgcggcaaag tgcaggccac gcggcgaatg ccgccaaaaa
     2341 ggggtggtga ttgcgcccgc aggcgcgtgt cggaacggtg cgaagcctcc cttgcgggca
     2401 accgcgctga aagcgcggct cttagcgcgc agacgccccc gcagggccag tgccgaggca
     2461 tgtgtagagc gaagcggccg ccgaaggccg ccgtgcgcgg cgaacggggc caaaggccct
     2521 ggtcgtaagc agtttggcgg aaaagcgcag cgtttttgac aagctgtcgc cgcctttgga
     2581 cggcgcgtta cgaggttttt tcgttttccg tttgcttatc ctgcgccgcg agctgcgctt
     2641 ccagtctgcg cacgcgctct tccaggaggg cgttttcctg cgcaaggcgc ttgatctttt
     2701 ccgcgaagcc ggagacgatg ctggaaaggc tgagcagcaa aagcaggatg aacgcgatga
     2761 caaggaggaa taccacgttc acgggcacag ccatgcccag caggtctgtc agcaccagaa
     2821 cgacataagg aaatactgcg aaaacgagca gcactccggc cgaaatgagc cagatgatgg
     2881 agtaccgcac cgtgagcttt ttctttttga gcagccacag gatgagggca agatagcaca
     2941 ccgcacccag caaaaggccg atacgcagga tagggctgat gctctgccag ttcatgcgcg
     3001 gccctccttc cgtctgccgg aggtgatgcg gaacaccacc agcgcaatgc tcaccttcac
     3061 catataataa accgatttaa aagggctgat ggacgatttg ccgcccatgc gctcttccat
     3121 gacgaccggg gcttcgccca cgcggtagcc gttcagaaca gcggtgataa tggcctccgg
     3181 ctccgggtag tcctgggcgt aatggtcagc aaaataggcg gtcagttcct tgttgcaggc
     3241 gcggaagccg ctggtggtgt ccagtatgcg cacgccgcac agaagacgga tcatcacgct
     3301 gatgaagttg atgcccagcc ggcgcatgaa gctcgtctga aagccctcct tcgtgataaa
     3361 acggctgccg atgcacatgt ccagcctgcc ctcggccacg ggccgcacca cgtccgccag
     3421 aaacaacggg ttgtgctggc cgtcgccgtc catctgcacg gtgacgtcat agccgtgctc
     3481 ccgggcgtat aagtagccgc tctgtacgcc gccgccgatg ccaaggttta cgggcagaga
     3541 aatgtaattg tagccgcggg ctctgcacag cgccgcgctg ccgtcggtgg agcaatcgtt
     3601 gacgatcagg taatccacgt ccggcgcggc agcgtgcagg cgttccacca cgcggtccag
     3661 gttttcttct tcgttgtagc aggggatgat cacaagggtc ttcaaaccgt cacctctttg
     3721 ggcgggcgcg cccgccgtat caagtagtaa tacgcggcaa aaaaaggttt tgttacattt
     3781 ttttaccgga aagcacaaaa tcctccagct gccgcagcag cggctcacgc cggaaatggg
     3841 tttcgtaata ggcccggccc gcatggccaa gggccgcgcg ccgctccggc ggcatatcgg
     3901 ccagggccag caggttttcg tacagtccgt gtacgtcgcc tgcggggctc gttaaaccgg
     3961 cggcggcttc gtccatcacg cgggcggctt caccgctcac cgaaacaagg catggccgcc
     4021 cggcggctaa gtagctggtg atttttgcgg gcaccgtcag tcccacgtcc tcgctggcat
     4081 tcagtgcggc aaacagtgcg tccgccatcg tgtgataggc agggatatcc gttacgggat
     4141 gctgtccctc gaaggtgaac caatccgcta catccgctgc ggcaatttcc tgctcaaggg
     4201 cgtcgcggct catgccgtct cccacgataa caaaatgcac gtcccggcgg tccgccgctt
     4261 tcagccgccg cgcgcattcc accagaagcg gcaggttctg cgcggggctg atgttgcccg
     4321 cgaacaccac gttgaaccgc ccggcaaagc ggctttccag agccgcgtca tgcacgtcct
     4381 gcgcatacag gtcttcacag tattggggca ccacggtgcg ctgcgcccag ggcgcgatgg
     4441 cggccaggcg ttcgtccaga gccgggctca tggccacaag gcgttcgctg cggcggtaaa
     4501 accagtggga aacgcccgcg gccacggcgc gcagcgcctt gtttcgcaca ggcatcacag
     4561 aatacaggtt ttccggccag aggtccagca cgtaggtggt gagcggcacg cggtgcagct
     4621 ttgcgtacac gacggcgggc agcatcatca gcacggggct ggtttcgtag cagaacacgg
     4681 cgtcatattt ccttccgtgc aggcgcggca gattgaacag cgccgtaacg gggaaggaaa
     4741 tataattgag gaagatgcgc agggaggtgt tgcccctgcg gcgtatttcg cccgcgcgga
     4801 agatctgcac gccggcatgg ctctcgcgcc gggggccggt ataacggtac ccctcaaacc
     4861 actcgccttt tggatagttg ggcaggccgc acaatacatc cacctcacag ccgtcttccg
     4921 caaagcccgc gcagatgtcg gtgatgcgga aattttcggg ccaatagtgc tggctcacca
     4981 caagaatgcg tttgcctttc acgcaaaaac tccttttttc agtgttccgg ggtgggaaaa
     5041 agacggcaaa gcatcaatcc ttgcgccata ccattttgtt tacaacgccc acatagctct
     5101 ggatgatctt caccactttg acggaaacgt cctcggtgta attgggtacg ggaatgccga
     5161 aatcgccgtt ttgattcatg gccacggcgg tagccacggc ctgctccacg ccgcccccgc
     5221 tgatgcccgc aaggataaag cagcctttgt ccaacgcctc gggccgttcg gtggaggtgc
     5281 ggatgcacac cgcgggaaaa gggcggccga cactggtgaa gaaactggat tcctcgggca
     5341 gcgtgccgga atcgctcacc acgcagaagg cgttcatctg caggtggttg taatcgtgga
     5401 agcccagcgg ttcatgcagc cgcacccggg cgtccagctt gaaattccgc tgctcgagaa
     5461 attttttgct gcgggggtgg cacgaataca gaatgggcat gtcgtaggct tcggccagac
     5521 ggttgacgga acggaacagg tcgagaaaat ttttctcggt gtcgatgttc tcctcccgat
     5581 gggccgagag caggatatag cgcccttttt caaggcccag ccgctccagc acannnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncttgcag cggttgcccg cctccatgtg
     5701 gaagatcggg atgtgcaggc gcttggctgc gatggcggaa agacagctgt tcgtgtcgcc
     5761 caaaatcagc agcgcgtcgg gcttctgctg cgccatcagc gcatagctct tgggaatgat
     5821 gttgccgatg gtctctccca gatcactgcc cacggcgttc agattaaaat ccggcgcgcg
     5881 cagttccaga tcctcgaaga aaatctggtt gagcgtatag tcgtaattct ggcccgtgtg
     5941 gaccagaagc tgattgaaat accggtcgca ttttttgatg acggcggaaa ggcggatgat
     6001 ctccggcctt gtgccgacga tggtcatgag tttgatcttt tccatgttca cacctcaagg
     6061 aaataggtat ccggatgctc cgggtcaaag ggctcgctgg cccacatcac ggtgaccata
     6121 tcactctcgc ccacattctc aatgttgtgg gtgtagcccg tgggaatgtc caccacttcc
     6181 agcttatcgc cggagacgcg gtattcgtac acggcttcgg tgttcagccg gcggaagcgg
     6241 atgatgccnn nnnnnnnncc gtaaagctgc cacgcgcatc gcagtgcatg tccagcggat
     6301 aggagaagcc gtctgtggga agataagaaa ggtatgtcgc atacaatttc cgtgtgaagg
     6361 cgtcgcccat gttcggtacg tccagcttgc cgcggctgcc gcggaagctt tcaatcagct
     6421 ccgccagacg gccaagcgtc acatcatagg cgtcgcacat cagatggcag tagccgggaa
     6481 tggagcagtc ccgcgcggca cgcccctcca tcgcggcgat gaagctggcc accacgtcgt
     6541 cgatgtatac c
//
LOCUS       DAWBSB010000126         6456 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_2287_length_6456_cov_3.415091, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000126 DAWBSB010000000
VERSION     DAWBSB010000126.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 6456)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6456)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6456
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2287_length_6456_cov_3.415091"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1335 a         1832 c         2049 g         1240 t
ORIGIN      
        1 agcgcacagg gtgcgcccag ccgcggcagc ggcggttttg gggttattat agcatgacgc
       61 cgcgaagcgg ttccaacaaa accgcacgtg ctgcggtttt gcatgctgca atgttcccaa
      121 aaccacgcgc gggagcgcgg gagcgcacag ggtgcgccca gccgcggcag cggcggtttt
      181 ggggttatta tagcatgacg ccgcgaagcg gttccaacaa aaccgcacgt gctgcggttt
      241 tgcatgctgc aatgttccca aaaccacgcg cgggagcgcg ggagcgcaca gggtgcgccc
      301 agccgcggca gcggcggttt tggggttatt atagcataaa aaaacccgga aagcaaaaaa
      361 tcctttgacg gagggcggaa tacttattat aatgagctgt aagcggcagc aaggcagctt
      421 atgcattata aaagtaatgt agaaagaggt gtccgccatg cgtcggatga acatgaacac
      481 ggcaggagcg gcgcgcattg gcgccgccgg ttttatgaac ggacaaagag cgggacggga
      541 tgcgccgctg tacggcggcc cgccccatga aggagaatta tgacggaaga accaatcaaa
      601 gtagtattgt ttgccgcgga ctgcggcgga tatgacattg agcgcagcat ggaagagctg
      661 gccgccctgg cacaggcaaa cggcatggaa gccgtggcgg aggtggtgca gaagcgtgaa
      721 acgccggagg cggccacatt tttgggcgaa ggccgcctgg ccgagggccg tatgctgtgc
      781 atcaacctgg gcgcggagtg cgccgtgttc gacgccgagc tgtccggcag ccagatccgg
      841 aatctggagg agctgctgga ggtgcaggtc atagaccgca ccatgctgat tttggagata
      901 ttcgcaagcc gggccgtgac gaatgaaggc aaactgcaaa cggagcttgc cacgctgcag
      961 taccgtctgc cccgtctggc gggccttggc gccagcctgt cccgtcaggg cggcggcggg
     1021 ggcggcggcg caggcgcccg ccgcggcgcg ggcgaaagca agctggaata cgaccgccgc
     1081 catgtgcgcc gccgcatcga cgcgctgcgc gaacgcctgg aagagctgga gcgccgccgt
     1141 gccgaaacgc gcaagagccg cgaaaagagc ggtgtgcccg tggtggcgct ggtagggtat
     1201 accaatgtgg ggaaatccag cctgctgaac cgcctttgcg gcgcgcaggt gctggaggct
     1261 gacatgctgt ttgccacgct ggacccgaca gtccgccgtc tgacgctgcc ctcggggctg
     1321 gcctgtgtgg ctgtagacac cgtgggcttt gtaagccggc tgccgcacaa tctggtgcag
     1381 gcgttcaaaa gcacgctgga ggaggccgcc tttgcggacg tgattctgaa agtatgtgac
     1441 gcttcggacc cggaggctgc cgcgcagctg gccgtgacgg acgacgtgct gggcgagctg
     1501 ggctgtggag atatcccgca gatcgttgtg tacaataaat gtgaccgtgt ggagaacgcg
     1561 gcgctgttcg atacttcggc cgtgcgcacc agcgcggtga cgggcgaggg gctggacgcg
     1621 ctgctggcac gcctagacgc cgcgttggcg ggccgcgtgc ggcgcatcgc ggtgctgctg
     1681 ccctatgata aactgggaga ggctacgccc atgcgggaga atggcactgt cctaaccgag
     1741 gagtaccgtc ccgaaggcgt gtatctggaa ggcattgtca aaacgatgga tctgcaccgt
     1801 tttacaccgt atctggtctg aggcggataa aacggcccgc gccgccggaa aggggcgcgg
     1861 gccgcaattt ttatatacaa agcaaaaagc gctgcttggc ggcagcgctt tttgcataga
     1921 ggggtgggaa agtataaata agtttggtgt aatcaaattc agtagcttta gtatactcct
     1981 tttgctttat ttgtcaaaag aagaaagcgg aaaaatgctt aataaaaaca tttacaaacg
     2041 aaaaaattca ttatataatt aagaaaactg cgaaagtgga ttcaaaatat ttattttgta
     2101 acttttcttt gaaaaacagc tttaaaacat taagatttta ccattggagg cttgtcccgc
     2161 atggatgaac tggaccgcaa aatactgcgc ctgctgtcgg ccgacggccg catgacggta
     2221 aaggatatcg cccgccaggt atcgctgaca agcccggctg tgagcgagcg catccgccgt
     2281 atggaaaaga cgggcgtgat cgcaggctac accgtagtgc tgggcgacgc cgagccgaaa
     2341 agccatatcg acgcgctgat cagtatttcc gtcgctccgg ccgacaggca gtatttcatc
     2401 acgatggtgg agaatcagcc cggcgtggcg caatgctttc atgttacggg cagccacagc
     2461 tttattgtga aggtgagctg cgcgggcatc catgagctgg agcatcttat caaccgtttt
     2521 cagaagctgg ggcagacgag cacccagatc attttatccg cgccggtgga ccggcgcttt
     2581 actttggatg tgcatgagca cggaaaggac ccggtatgat ctgcgagaaa tgcggcgaac
     2641 ccattgcgca ggacgcgctg tattgtgaaa attgcggggc gcgcgcggcg ctgcaggcgg
     2701 acgcggcgca gaacgcggcg caggcgcgtt ccgcgtctgc gcggaaaagc atgcctttgg
     2761 acgaaggcgg aagcccggag caggaaaacc cggcgccgga ggatgctgcc cggcccgggc
     2821 ggaagcgccg ccgcgcaaag cgtgaaaaaa tgccgcctgc cgaccctccc atcccgccgg
     2881 aagatatgat ccccctgaca acagggcaat gtgtgggtat cctgctggcc ctctgcgtgc
     2941 cggtgctcaa ccttgttctg atgctgaaat gggcttatgc ggcccggggc aacgagagcc
     3001 gacggagcct ggcgcgtgcg ggactgatcc tcacgggcgt gtgtatcggg gcatccatcg
     3061 tggtgctggc ggccgttacc tttgcggtgt ccatggggct ggtatctgtg ggcggtgtgg
     3121 tttgagcatg ctgcgcgcgg gagaaaagga gcattctgaa gaatgattgt acgaaatgaa
     3181 aaagaaaccg accacaggca tgtggaagag ctcataagaa aagcgtttta caacctttat
     3241 gtgcccggct gcaccgagca ttatctggcg cgggtcatgc gcggccacga ggactttatt
     3301 ccggagctgg acctggtgct ggaactggac ggccgggtga tcggcagcat tctgtacaca
     3361 aaggcgaagc tttccgacgc ggcggggaag gaaaaagaga tcctcacgtt cggccctgtc
     3421 tgcatcgcgc cggaatatca gcggatgggc tatggcaggc ggcttatgga aacgtctttt
     3481 gaaaaagcgg ccgcgctggg ctatgacgtt attgttattt tcggcagtcc ggcgaactat
     3541 gtggcccgag gctttaaaag ctgcaaaaag ttcaacgtct gcgcggataa cggacgttac
     3601 cccgcggcca tgctggtgaa ggagcttgtt cccggcgcgc tggatgggcg caaatgggtc
     3661 tatcgcgata gccctgtgat ggcgttttca gaggaagaag cccaaaggta tgacgatact
     3721 ttggaaaagc tggagaaaaa atggcagccc tcgcaggagg aattttatat tttatcccat
     3781 tcctttgtcg aataggacag agcatatgcc gccggatgcg tgcaggctga ggaggcgttt
     3841 cgctgcgttg aacgaggcat aagtgaaaga gtattccgga agaatggggt gacgactgcg
     3901 cccgcaggcg cgtgaggctc cgttcgtaaa gcagttgggc ggaaaagcaa agttttttga
     3961 caaattgaaa cgccggaacg gcccgcagac cgttccggcg tttttctgtt tcagatgctg
     4021 cggagtgtcc gcgcttaaaa cgcgatgtca tcctcggcgt catccgcctc gtccgctgcg
     4081 gcggtttctt ccgccgcagg ttcctcatgg ctgaaatcct cactgaacag gagctgctca
     4141 tattcatcca gctcggcctc gcggcggcgc agatacatat aagcggcagt cagcgcgccg
     4201 gcaatggcag cgaagaacac caaaatggct gcaaaagtcg attttttcat ggtgataacg
     4261 tcctcctttt gggggcgcag ggcttcccgc acccgttttt ctattttttt gccggtgcgg
     4321 taggcctctc ccgcggccag agcggtttgg gccgccccaa gtgcaagcct cgtcagtttt
     4381 gacgccagaa aaagtcccat ccgcaccctc ctgcacaccg attttcactt acgcttatta
     4441 tatccgcttt ccggccggtt tacaagtatt ttttcaacac cggacggtat gccttgaacg
     4501 cgcccggcag tgtatacact ccgtccactt ccgcgcgccc ggcccagacg cagcccggcg
     4561 gaggcgcgca gctttgcgcg gcgcaggcaa acccgtccat ctcccacaca agatggctga
     4621 acacatgccg ggcgggcgtc aaaggctcca cggcgcgggc tgtgacgccg agcgccgcca
     4681 gtgcctgctc tgtttgtatg gcgctgcgcg cgccctccag cagcacgggc tgccacagcc
     4741 ccgccagaag ccccttttgc gggcgctgct gcagcaggac gcgccggttt tccatatcca
     4801 gcaccagcgc cacggtccaa tgctgcgttc tttttgcagc gggcggcgcc tttacgggca
     4861 gtgtctcctg cacgcccagg gcgcgggccg cacacagccc gcgcaaggga cactcgctgc
     4921 acaggggcgc gccgcccggc aggcacacca atgcccccag ctccatcagc gcctggttgt
     4981 agtcgccggg gcgttccggc ggctgcagct ccatcacgca agctgtcagg tcttttttta
     5041 cgtcaggcct ggtcacatcg cgcccatcgt tcaggatacg ggcgaacacc cgcagtacgt
     5101 tgccgtccac ggcgggcacg ggcagaccaa agctgatgga ggcgatggcg cccgccgtat
     5161 atgcgcctac gcccggcagc ttcagcagcg cgtcataatc ggcgggcagc gcgcccccgt
     5221 gctgctccat tacgatctgc gcggcctttt gcagattccg cacacggctg taatagccca
     5281 ggccctccca tagtttgtgc aggcgctcgg ggtcgcaggc ggcaaggtcg gccactgtgg
     5341 gcagctcggc catgaaacgt tcaaagtaag gcagcgcggc cgagactctc gtttgctgca
     5401 gcatgatctc gcttacccaa acgcggtagg gcgtggagac cgtgcgaaac ggcagagtgc
     5461 gtttgttctg ttcataccac cgcagcagcg gcggcgcgat atcgggaagt gtcattgcca
     5521 gtctctccag tgctcggcca gccagcgggc cacgccgttt tccgtgttgg cgccgatgac
     5581 gcgtgtagcg gcggcgcgcg cttcgggcac ggcgttggcc acggcgcagc tctccgcgga
     5641 gatgcggaac atcggcacgt cattgatgtt gtccccaaag ctgacgatgc gccgcgcgcc
     5701 cgtcatctcc atcacgcggg ccagtgcgct ggccttggtg cagccttcgg gatagatttc
     5761 cagcaggcag ccgccgctgc cgtattcgtc ggaataatag tagcagataa gcccgggaat
     5821 ggccgaaaag atatcgtaaa acgcgcgggc ttcctctgcg ccgggcagcg caagaaggaa
     5881 catcagcgtg tcgccgcccg caagcgcttc gggatagtgt tccacctgca caaaggtctt
     5941 atacggagaa tggatgcggc tggaaacgaa cgaccgctca aaatcgcagg cgaatttttt
     6001 gtaatacaca tacaaatggc cgcctttgac gctgtaagca agcgcctcct ggcccgtgcg
     6061 ctccaacacc gcgcacacgg cggcggccgt ttcccgcgcc agcggcgtgg cggcaaggca
     6121 gcgcgtgtgc gcaacatcgt aaatcatcgt acccgtcatc agcacagcgg gcgtgcgaag
     6181 gcccagcggc cgcagcagct ccaccaccgt ggcggggctg cgggccgtgg ccacggcaag
     6241 ctgaagccct tcgtccagca tagggcgcag caatgccgcg ctttcctgtg aaatgcgcgc
     6301 gtctgcgccc agcagcgtgc cgtccagatc cgtcacaaac agggtggaca agccgcccgc
     6361 ccccctttct tcagcaattt tttctgttct gccatatttc caagctcgca cttcgcgtac
     6421 cgctcgtgtt cactaagaaa catggtatct atgccg
//
LOCUS       DAWBSB010000127         6429 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_2298_length_6429_cov_3.235645, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000127 DAWBSB010000000
VERSION     DAWBSB010000127.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 6429)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6429)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6429
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2298_length_6429_cov_3.235645"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1260 a         1847 c         1951 g         1371 t
ORIGIN      
        1 cgtttcgacc tccagcttaa acacgcggac ggaatccaac atcgcgccgg aaaacgtcca
       61 gccgtctttt ccggtattgt gcagcatcag agcctgcaaa gccgcttctt tctgcgcggt
      121 ttcctccaca aaggaaacgc gccccgtgcc gataacgctt tgaaaccggg cggtatgccg
      181 acaggcttcc tcgcactcta ccagcgcgta gtttacgtcc agctcaaagc cgacggaggg
      241 cgccccggcg atgagatcca gcttccgccc gcttttagcg ctgtgaaaat agaacacccg
      301 ctttccgttt tcctcggcgt agccgaaatt cagcggcacg atatacaccg cgccgttgtc
      361 gcagaagccg aggcggcagc aatggcaggt ctggatgatt tgtgtgattt tttctgcatc
      421 tgtgatctcg cggtccgtac gccgcatgtg cctcactcct cattggtttt gtttcatgat
      481 aacacaaagg aaccggttga ggcaacttta caaaatatgt gttgcctgtg ccgccggcag
      541 gcatggaaaa tcaaattttt tgatttacgg ccgtggaagc ccggcggatt tgcggccgcg
      601 ctgcccggcg cactgctctg cttttgcaaa aaacaacgga atcttagggg agacgggctt
      661 tttaacttga ttcgacaccg ttcgacatgg taaaatagac tcccggcgca tcccacttcg
      721 cgccggccat ggccgcgtct gttccccatc caggaatggg cgcggccgct ttgtttttta
      781 ccggggaaaa ccgtgaaaag cggttgattt ttgcgctccg ttcgcctata ctgaaagtga
      841 aaaaagctgc tgtttcaggg agggaacgtt atgaaaaatt acgtgatcac catcgccagg
      901 gaaaacggaa gcaacggccg cgccatcggc gaggcgttgg ccgggcgcct gggcatccct
      961 ttttacaacc gggatatcct gcgcatggcc gccgatgaca gcggcatcaa cgaacaactg
     1021 tttgccaacg cggagaaaag cttccgcagc agtctgctgt tccgcgtggc caaggacgcc
     1081 tacaacggcg aggtcatccc gccggacagc gacaactttg tttccaacga gaacctgttc
     1141 cgctaccagg ccaaggtgat gctgggcctg gcgcagaacg agagctgtgt gatgatcggc
     1201 cgctgcgcgg actttatttt gcgcgaccgg cccaatgtgc tgcgtgtgtt catccacgcg
     1261 ccggaggaag tgcgcgtgcg ccgcgtcatg cagcgccaca acctctctga ggccgaggcg
     1321 cgccgccaca tgcgccaggt ggacaaacgc cgcgcggact attaccgcgc tttcacgggc
     1381 agcgactggc gcgacgccgg ccattatgac atctgcttcg attccgacca tatgggtgag
     1441 gagaagatca tccgcttcat tatgggatat atgaacgtgt gcttcgagga ctgagccgcg
     1501 gcggaaagaa aatgaaaaca gccggaaggg aagattccct tccggctgtt ttcatgtccg
     1561 gcgtcaggaa agctcgaact taccggtgta gagctgataa tatctgcctt tttgcgcgag
     1621 cagctcactg tgggtgccgc gttcgatgat acggccctgt tccagcacca tgatgcagcc
     1681 tgaattgcgt acagtggaga ggcggtgtgc aatgacgaac accgtgcggc ccgccatcag
     1741 gcggtccatg ccctcctgta caatgcgctc ggtgcgtgtg tcgatggaac tggtggcctc
     1801 gtccagaata agcacgggcg ggtccgccac ggccgcgcgg gcaatggcca gcagctgccg
     1861 ctggccctgt gagaggtttg ccccgtcgcc tgtcagcaga gtgttgtagc cgtccggaag
     1921 atgttcgatg aacgcgtggg cgcccgccag ctgcgccgca gccgtcacct ctgcgtcggt
     1981 ggcgtccagt ttgccgtagc ggatgttgtc cgctacggtg cctgtgaaca ggtgtgtgtc
     2041 ctgcaaaacg attcccaggc tccggcgcag atccgccttt ttgatcttgt tgacattgat
     2101 gccgtcgtag cggatcttgc cgtccgcaat gtcataaaag cggttgatga ggtttgtgat
     2161 ggtcgttttg cccgcgccgg tggctcccac aaaagcgatc ttctggccgg gcctggcata
     2221 caggctgacg ttgtgcagaa tgatcttggc gtcgtcgtaa ccgaaatcca cgtcgtcgaa
     2281 caccacttcg ccctccagcc tgcggtaggt ggtgctgccg tcggccctgt ggtaatgctt
     2341 ccaggcccag acacccgtgc gcccgcggca ttcttccaga ctgccgtccg cggtttgttc
     2401 cgcattcacc agctccacat agccttcgtc ggtttcagga ggctcatcca tcaggctgaa
     2461 tacgcgctcc gcgcctgcca gcgccatgat gatggagttg aactgctgag atacctgtga
     2521 gatgggctgg ttgaagcttt tgttgaactg cagaaaggat gccagcccgc ccagcgtaaa
     2581 gccgcccgtg ccgaagagcg ccagcagcga gccgaggatg gccgtgagca catagctgag
     2641 gttgccgatg ttggccatga cgggcatgag gatattggca tagcggttgg cgttatcggc
     2701 actgtcggcc agcgcgtcgt tcagcgcgtc aaaccgggcg atgctctcat cctcgtggca
     2761 gaagaccttg acgaccttct ggccctccat catctcctcg atatagccgt tgaccgtgcc
     2821 cagattgcgc tgctgcgcta cgaaatatgc gccggacctg ctcgtgagct ttttggtggt
     2881 gaacagcata aggcacacca tcaccagcgt caaccctgtc agcggcaggc tgagggcaag
     2941 catggagcag aatacgctga cgatcgttac cgccgaagag acgagctggg gcatgctctg
     3001 gctgatcatc tggcgcagcg tgtcgatgtc gttggtgtaa atgctcatga tatcgccgtg
     3061 ggcatgggtg tcgacataac ggatgggcag gcgctccata tgccggaata aatcgtcgcg
     3121 gatggcgcgc atcgtcccct gggacacata taccatgatg cggttgtaca gcagcgtgca
     3181 cagcacgccg aacaggaaga cgcagcccat tttcagcagc gccgccagca gcgggccgaa
     3241 gtccgacgcg ccgccctcgc cgatataggg cacgatgtag tcgtcgatga gcgttttcat
     3301 aaacagattg cccgccacgg tggccgcggt gctgccaaag atgaacagca gcacgcccag
     3361 gcagtgcagc ttgtagcgcc gcaggatata gccgaacagc cggcccatga tgcgcatggg
     3421 cttttcaacc ttttgctttg gggccatcat ggccgcgctt ttatcagcag gtacgccgcg
     3481 catcgtcctt cgctcctttc atctgggaat catagacatc ccggtaaatt tcgctgctcg
     3541 caagcagctc gtcgtgcgtg ccgaaggcgg ccacgcggcc gtcgtccagc acgaggatgc
     3601 ggtctgcgtc ctgcacggag ctgatgcgct gggcgatgat gaatttcgtg gtgtcgggga
     3661 tctcctctgc gaaagcccgg cggatcaggg tatccgtccg ggtatccacg gcgctggtgg
     3721 aatcgtccag gatcagtatc ttgggctttt tcagcagcgc gcgcgcgatg cacaggcgct
     3781 gtttctgccc gccggacacg ttggtgccgc cctgctcgat gtaagtgtca tagccgtccg
     3841 gcagcgcgcg gatgaactcg tccgcctgtg ccaggcggca ggcgcgctcc atctcctcgt
     3901 ccgtggcgtt tttgtcgccc cagcgcagat tttcccggat ggtgccgctg aacagtacgt
     3961 ttttctgcag caccatggcc acctcgttgc gcagggtttc cacgtcgtat tcccgcacat
     4021 ccacgccgcc cacgcgcacc gcgccttccg cgacgtcgta caggcggggg atgagctgca
     4081 ccaggctgga tttcgcactg cccgtgccgc cgatgatgcc gatggtctcg cccgcgcgga
     4141 tgtgcagatc aatgtcatcc agtacgggct tgccgctttt tttgtaggag aaggatacgt
     4201 ggtcgaagtc cacgcggccg tcctccacct gtgtgcgggc ggcgggcggg gaggtgaggt
     4261 ccgccttttc ctccagcacc tcggcgatgc ggtccgccga ggcgcgggcc attgtcacca
     4321 tcacgaaaat catggagagc atcatcaggc tcatcaggat gttcataacg taggtaaaca
     4381 ggctcatgag ctgacccgtg gtgagagaat gggcggcgat catgtgcgcg cccagccagg
     4441 aaatggccag gatgcacatg tacatcgtga actgcatcac ggggctgttc agcgccagca
     4501 cggtctcagc cttgacaaac agcctgtaaa cattgtcgtt ggcggtgtgg aatttctgcg
     4561 tctcgtggtc ctcgcgcaca taggccttga cgacgcggat ggcgctgaca ttctcctgca
     4621 cgctggcgtt gaggtcgtcg tatttgcgga atacctgccg aaatacgggg tgagaatttt
     4681 tcgtgatgaa aaacagcgcc acgcccagaa acgcgatggc cgccacgaag atcagggaca
     4741 gccgcgcgct gatggagaac gcggccgcca ttgcgcacag cagcatcacc ggtgcgcggg
     4801 tgcacatgcg caggatcatc tggtaggcgt tctgcacgtt tgtcacatcc gtcgtcatgc
     4861 gggtgacgag cccggccgtg gagtatttgt cgatgttgga aaaagaaaat gtctggatat
     4921 tgcggaacat ggactggcgc agattgtgcg cgaatccgct ggaggcccgc gcgccgtatt
     4981 tgcctgagag cacgccgcag gccagagaca gcattgccat cacggccatt gcgccgccgg
     5041 tgatgcagat cgtgcgcacg tcccctttct ccacgccgct gtcgatgaga taggccatca
     5101 gcatggggat gagaatctcc atgacaactt cgcccaccat gaatgccggc gtgaggatgg
     5161 acgctttttt gtactgcttt acttcagcca atagggtttt cagcataaaa tgtcctcctg
     5221 agattggttt tgtttcgctt aacttgttag taccattaca gtaagcaccc taactatata
     5281 cgccgaggaa aaccgctggc ggcaggcacg gaaaaagccg cgcctgcgcc ggcggcatgg
     5341 aaaaggcaaa gcggacactc actccagatt gcggcggaat ttggctgtga tggcgaaaaa
     5401 ttgctccagc tcttcgggcg tgacgtcttt ggtgatgcgt gcctctgtgg cgcggatgcg
     5461 ggagatcacg ccctcgtgca ccgccaaagc cttgggcgta agcaccagct ttttcagccg
     5521 ggcgtcatag gcaacaggct cccgcagaag aaagccgttt ttttccatca gctgcaatat
     5581 gccggtggcc gtggagcggc ggatgttgaa ttcagcctcc acgtcccgct ggaacatgtc
     5641 acggtcctcg ttgcggtgca aaaagccgat gatccaccct tgcatgccgg tggtctccgg
     5701 cggcatgccg tcgtccatac agcgtttgat caggttggaa agcacgcgga tctctccgcc
     5761 cacgtggcgt tcacccttca tccttcgaca cttccttgct ttttagttag gctactaaca
     5821 attatagggc ggatccgccc cggtgtcaat ggctttttaa gaatttatta taaaaaaggt
     5881 gacgctgtga gcggccgcgc ttcaaaggat gttcagaatg ccgtaagatt tttgaaagcc
     5941 tttccccagc ccgtccgtca ggaacgtgcg gtacgatggt cctgttctca acaaacaatg
     6001 ggaaggggcc atccaaatgg aaaaaataaa agtcgccgtg ctgttcgggg gacagtccac
     6061 cgagcatgcg gtctcgctgc aatcggcctg cgccgttctg cacgccatga agggtacgcg
     6121 ctttgaagcc gtgccggtgg gtatcacgcg ggagggcagg tggttctggt accgcggccc
     6181 gctggacgcc gtaggggagg gcgtatggga aaaagaggaa tgtgcgcctg tgacgctgac
     6241 gccggacgct tctgtccacg gcctgctggt gcagggcgcg ggcagcctgt atctggatgc
     6301 ggtgtttccc gtgctgcatg gaaaaaacgg cgaggacggc accgtgcagg ggctgcttgc
     6361 gctggcgggc atcccctgcg tgggctgcgg cgtgctggca agcgccctgt gtatggataa
     6421 ggacgcagc
//
LOCUS       DAWBSB010000128         6422 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_2301_length_6422_cov_3.448092, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000128 DAWBSB010000000
VERSION     DAWBSB010000128.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 6422)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6422)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6422
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2301_length_6422_cov_3.448092"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6144..6153
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1244 a         2042 c         1782 g         1344 t
ORIGIN      
        1 attgtgggta tcgaaatagc ggatgggaag gtcctgcatg ccgttgaaca tcttctcgcg
       61 cattttttcc agcgagccct gcgtgatgac ggccatgagc tgagtatata caacacccgc
      121 tgccaggctg aggacataaa acaccgccag caacgccacc agtcccaaaa tgcggccgga
      181 tacggcggac caatcgccgc tctgccagct ctgctccacg gcggcaatga cgttctgcat
      241 gaagatggac ggcaccgaac tgacgacggc gttgaacagg atgcacgcca cggtaacggg
      301 cagcagcacc ggataaaaac cgaacagcgt gcggatgaga cggcccagca cgcccttggg
      361 cgcgcggcgc tttgcggttc ctttattcgg catcgtcgtc acctgccttg ttctgagagg
      421 tatacacctc gcggtagatt ttgtttgttt tcataagctc cgcatgggtg cccacggcat
      481 tcaccgcgcc gccgtccatg acgacgatgc ggtccgcgtc ctgtacggag gcgatgcgct
      541 gggcaatgat gatctttgtc gtttcgggta tataccggcg cagcgcctgg cggatcagtg
      601 cgtcggtgcg cgtatccacg gcactggttg aatcgtccag gaccaggatc ttgggctttt
      661 tcagcagcgc gcgggcaatg cacaggcgct gcttctgccc tccggaaaca ttggcgccgc
      721 cctgctcgat atgggtgtcg tagccgtccg ggaactggcg gataaactca tccgcctgcg
      781 ccaggcggca ggcctcctcc atctctgcat cggtggcgtc cttgtttccc caacgcagat
      841 tttcccggat ggaaccgctg aacagcacgt ttttctgcag caccacggct acctggtcgc
      901 gcagagcctc caggtcgtag ctgcgcacat ccacaccgcc cacgcgcaca gagccctcag
      961 tggcgtcata cagacgggag ataagctgga tcagcgtgga tttggacgag cccgtgccgc
     1021 cgataatgcc gatggtctcg ccggaacgaa tgtgcaggtc aatgccggaa agcgccatgc
     1081 gctccgccgt ctttgcgtac ttgaagctca cattatcaaa atcgatggag ccgtccttca
     1141 cctcgcgcac agggttttcc gggctttgca gcgcgctctc ttcgcacagc acctccacga
     1201 tgcgctgagc cgattcactg gccatggtga tcatcacgaa caccatggag agcatcatca
     1261 ggctcatcaa tatctggaag ctgtacgtga gcatggagga catctggccc acatccagcg
     1321 caaggccgcg gctggtgatg accgtatacg agccgaagga cagcacgaac accatcaccg
     1381 catacacaca gaactgcatg agcgggttgt tgaaagcaag gatgcgctcc gctctcgtga
     1441 agtcccgctg gacgtctgct gccgccgcgc cgaatttctg tttttcatat tcctcgcgca
     1501 cataggactt cacgacacgc atacctttga tgttttcctg cacagagctg ttcaatgcat
     1561 cgtatttttt gaacacgcgc ttgaacagcg gcatcgtgct gcgcaccacc agcgccagcc
     1621 ccacgcccaa caccgggatg accagcagga agatcaccgc catcttgccg cccatgataa
     1681 acgccatcgc aaacgaaaac accagcatca gagggcagcg gatggccgtg cgcacgatca
     1741 tcatatatgc catctgcacg ttggtcacat cggtggtcat gcgtgtcaca aggctggaca
     1801 ccgaaaactt gtcgatgttc tcgaaagaat aatcctgtat tttatagaac atgtccttgc
     1861 gcagattgcg cgcgaagccg cagcttgcgg tggcgcaggc gctgcccgcc agtgcgccga
     1921 aggtgagcga cagcccagcc atcaccacca gcaccgcgcc gtactgcaca atgacgccga
     1981 tggagcaccc ggctttgatc tggtttacca gattcgccgt gacaaacggg atgatgcact
     2041 ccatcaccac ctcgcccgag accaacagcg gcgcgagcag cgaaggcttc ttgtactcgc
     2101 ggatgctttt ggccaaaacg ctcagcataa aaagataccc ctttctctcc ggctccgagg
     2161 agcctttctg tattatttgt tatgacgcta acaattcggc tcaaaaaaat cagccgcagt
     2221 ctcttcggcc gccctgcgac aggcagtgcg gcccgacgcg gccatgcggg ctgccgcccg
     2281 cggctcttga attttaagcg ccagctatgc cccgtttgtt aggggacgaa ccatatctta
     2341 ctgctttttt ttaggtttgt caatagaaaa agggtacaga aagcgttcag ctttccgcac
     2401 ccttttgccc ctttggcagc cggttattgc tccttgtccg cacgcaggcg ccgggtcacc
     2461 tcccgggcaa cacgcaggcc gtgctgtgca ttttcagcgc ccagccgtgc aagcgggtcc
     2521 ggcgcgccgt gcagcgtgcg ctccagccat tccgcctcca gcgcctgcat ccgcgccagc
     2581 acctccggat accgggcctt tacatccgcc gtttcccgcg ggtcctcttc cgtatgatac
     2641 agggcatatt ctgcaaaatc ccacaggccg gggctgaagg tatgcaccat cttccagccg
     2701 tcctgtaaaa ccgccctctg gcaggtatac agcccgtgct cacacacaag atattcccgg
     2761 ggcgccgctg ttccgtccag cgtgtcctgt agcgctgtac cgtgccacat ctccggtgcg
     2821 gccacgccga aaaagcgcgc cgcggcaggc gcgatgtcca gcccatacac aggatgggcg
     2881 cacacctcgc ccctgtgggc gccgcccggc agcttgagca gcatcgggat atggatacag
     2941 ctgtcatgca ccgtgccgtg ctccaggatc atctgcccct ctccatactg ctcgccgtgg
     3001 tccgcactca gtatcacggc acagtcgtca taccagcctc tttttttcag gtatgcgcac
     3061 atctgcccta ccagatggtc ggtatagcgg atttcggcgt catacaggcg aagcagctcc
     3121 gccagctccc gtcggctgcg cacgcccgta tgccggccgc cccggtacag cggagatgcg
     3181 gccagccggg tgagctgttc atcggttacg gcggaggtgt ccggctcata gccgcggtcg
     3241 atccattccg gcggcgggga aagcggcccg tgaggatccc agtaatgcag atgcaggaaa
     3301 aggtcttccc cacaaaagcc gtccagaaaa tccattgccg cctcattcac cgcggcgccc
     3361 ggaaagcgct ggaaattttc cgcacggcag cggtaggagg ggtcgatcac atgggaaaat
     3421 ccattgtaaa accatggcgc aggatgccgc ccgaacgaag aaaccgccgc cgtgtgcacg
     3481 ccctcccagg acagtacacg gggcagggcc gtttccggcg gcgtggccac ggaaagtgca
     3541 gccgtaccgt gggtctccac gccgttgacc acgccgaagc acccggtaaa cagcgctgcg
     3601 cgggccggca tgcagggcgt gttggaagca tacgcccggg aaaaacgcac cccgtccgcc
     3661 gccagcgcgt cgatgtgcgg cgtcggccgc ccatacccgc agcaggacaa atggtccggc
     3721 cgcagcgtgt cgatatcaaa atagatgacc ttcatcgtac tttccttaca aaatgcctgc
     3781 ggccgtgctc atacagggcg cagatcattt cctgtgtcaa aaaggcgttt tccgcggtga
     3841 catacatggg cgcaagcccc agcaggctct gataaaagtt accgatctgc ttgacatggc
     3901 tggtgcccca atattgcttg acgtccccgt attcaaacat ctgtgccggg tcgttgtccg
     3961 caaggcaggt gaggccgttg ttcagctcca cccgcgcctt gggcccttcc ataacggctt
     4021 tgccccgggt gcacgccagc tccagacgaa ccggctcgtc tgtgcagtag tggttcatca
     4081 cataaaaggc gccgtacgca ccgccttcaa attccacccg tccctcggct gtgtcctcca
     4141 ccggaatatc cgggtgcgtg cggttggcca ggctggcctg tacgcctgtc accggacggc
     4201 cgatgaacca gcgcatcagg tccagcgtat ggatggcctg gtcgatgatg acgccgccgc
     4261 cctctttatc ccaggtgcct ttccagtcgc tttgggaata atattccggt gagcggtccc
     4321 atgtcagcag cacgcgcgcc gcgcgcacct cgcccaaatc tccgttgtcc agacaacggc
     4381 ggatgagctg ggaacccgcg ttatagcggt tctggaaaat gacgcccagc gttctgcctg
     4441 tggcggccgc ggtgtccagc atacggcgcg cgtccgccag cgcaatggcc atcggctttt
     4501 ctgtcagcac atggcagccg tgctccatgg caaggcacgc cagcggcgca tgcagatagt
     4561 gcggcgtgca gatatgcacc acatccggct tttcctgctc cagcatcact tcaaaatccg
     4621 tatacgcccg gcagccgaag cgctccgccg cagccccggc acgctcgggc ttcacatcgc
     4681 acaccgccgt gagcacggcg tgcgggtccc tggagaccgg tacggcatgc atggggaata
     4741 tctttccgca gcctgcgatt accgttttca gtttccgtac ttccatcact tcgtcccctt
     4801 ttggcatcag gccgccccaa ggcaagctat tgccttgaaa cagcctgatg gatgagcggg
     4861 gcgcaccgga gcgcatcccg ccgtttctgt tttttttctg ccgctgcaga aattcaggcc
     4921 tcccgctctt tcacgaagcg ctcgaaccag atgtacgcca cttccttgcc cggctccgcc
     4981 gtcagggaat ggtccagccc ggcgggcacg aagctccagt cgcccgcatg atgggccacg
     5041 ctctcgtttt ccaccgtcag cgtgaaatcg gcattgtcca ggccgtagtt ccactggtcc
     5101 accgcgggat ggcctttttc cacagtgcct tctcccacgg ctttcaccac gcccagcaga
     5161 ttgcggccta cctcgccgga gtacagcacc tgccagctgc gggtgtgggg gcccttgcag
     5221 tcctgcgtat actcatgcgt ctcagagaag gaacggaaga tcggcagcgt tgtgtggcac
     5281 gccgcataat ttttgtggtc tccctctgtc atatccagca ccaggcacag gaactccaga
     5341 tcctcgacgg cgtgaatggt aaaatcgccg ttgtcaaaat ccggcacaaa gaaagccagt
     5401 tcggtgatgt tgcgggcgaa cttatccgta gtgacatagc ccttgccggc tgtaaagcag
     5461 tacactgcgg tcttatctgc atatttttcc ggctgcacat catgcccggc cttcaaaaag
     5521 catttgtagt tgtgggcgcc ggggaacgtt ccgggcagca tttccatgcg ggaaaatccg
     5581 tttgcgtcat actgaggcgc catttcttcc ttttttacaa tcgcgataga cattgtgttt
     5641 accctctttc ttgttttttc cgtgccgggt acagcgtgcg ccgtcccaag cggcggtttt
     5701 tccgttttgc gtctgcgccg ggcctctgct ttacagcccg gcgtatatgg gcgagacaaa
     5761 cgcccactgg ccgttcttct gggcaaattc cgcatgatac acgtccatgc ccccgctgcc
     5821 ctcgtcctct gcctccagca ccatttcata ggcgctgtcc ggcacggcgg catgaatttt
     5881 taccgcctcg gaatgccagg gcttcacgtg ccaggttacg ccgccgtcca aaagctgcgc
     5941 gatggacgcc gtgtgcgtac ggccgtttac cgtcacgttt accacagtgt ccggcccgcc
     6001 ctctatctcg gcaacgacgg cgtccgtctg ggggtgcagc gtgctcttgt tgcgcacggt
     6061 ctcgcactgc caggcaaagg cctgcgtgtc ctgttccagc acacggttgt cgatctcgtt
     6121 tacctcgaca gcgcttccgt cgtnnnnnnn nnnacctgga gcggcacaaa tgcgtcgcgc
     6181 gctgttttgc tgttgttcgt gccgttcagc agatcgtcca caaatgtctt gtgatgaatg
     6241 tacccaaaga actgcgggtc gacgggggcg acctgccagt ggatatcctt gcggggatgg
     6301 ccgtccagat tccagccata atatacgata tcgctgcggt ggggattgta cagatagata
     6361 acacccttcg caccgtagat ttccatttca aagcggttgg tctgcgccac ctgcgtccag
     6421 aa
//
LOCUS       DAWBSB010000129         6357 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_2333_length_6357_cov_3.122977, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000129 DAWBSB010000000
VERSION     DAWBSB010000129.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 6357)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6357)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6357
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2333_length_6357_cov_3.122977"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6111..6120
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1209 a         2085 c         1647 g         1406 t
ORIGIN      
        1 aacaaaagcg gcaccacata gcaggtaatg agcagcgtcc tgctgcggat gtccagccgc
       61 cattgcagcg ccgtgccata caaaaacgcc cccatcatac atcctccttc gcaatatcca
      121 taaaatgttg ctccagtgtt ccccgtccta ctgtaacatc cagaaccgcg acgccgcacc
      181 gtttgtaagc ttccagcaac ggcagcaggg cctcccctac gtcgtcagcc tcaaaacgct
      241 cctcaccctg cgctgtccgg acgcacacaa ggtatcggct gccgatatgc gcggtcagtt
      301 ccgtcacagt gcccagaaaa accaactctc cgcggttcag gatgccgatg cggctgcaca
      361 ggctttccac ctccgccata tcatggctgg ccaggacgat ggttttcccc tgcgcctgca
      421 gtgcgcggat ctgcgcgtgg agcgccaccc gcccctccac gtccagcccg gctgtgggtt
      481 catccagaaa taccacgtcc ggccttccgg tgagcgcaag tgcaagatgc agccgccgct
      541 tttgtccggt ggacagcgcg ccgtactgcg tttttttcat cccaccgatg cccagtgcgt
      601 ccagcatggc cgcatccggc ttcgttccgt tccattttgc aaacagcgcc acggcctcca
      661 tgggccggat gtgtgccggc agagaggcgg actgaagctg tatcccggcc ttgccccaca
      721 ccacagcact gccgccatcc tgccgccgca ggccctcgat gcattccagc gccgtggtct
      781 ttcccgcgcc gttcaccccc agcagcgcaa agatctcgcc ctgctccacc gtgaaggtca
      841 gtcccttcag caccgcacgg cctgcgtaac ttttttgcag gccctccacc cgtatcgcac
      901 agctcattgc atatcctccc ctgtcggccc tgtcccgatc cgggtaaaat aggcgtccag
      961 cgccggctcg atgtacgcac ttgccgcggc ctgcctttgc cgccacacgg cgtccgctcc
     1021 gtcaaagctt cccatctcca tcagctttgc caaaatttcc atgtccccgc cggtgaactc
     1081 cgtcaccatc tgccaaaatg cggccgccag cgcctgcccc cgcgcccctt cgggcggctc
     1141 gcctgcggcc tgcagacgca gcgcctcgtc ctgcaggcgg ttgaaggtct ccagcatcgc
     1201 ccggccgctc tctttgtcga agcggctgcg gatatggtcc agcgttcttt cgtcaaaatg
     1261 cttgatgagc cagtaaaact catttttcat ctgcaggttc acaataatat ccgcgtactt
     1321 tttaaagtcc accgtctgca tctgcagcac ctcggcacgc aaagtctcaa tggccgtgag
     1381 tgattccgtc agcaccgcga tcttctcccg cacggcggca gcctgcgctg ccagcgcgtc
     1441 cgcaacgtcg gcgggcgtat ccaacggcgc aagcctgttt ttgatgtcgt ccagggaaaa
     1501 acccagagac ttcagcgaca ggatttgatg gagctgtatc atatccttat ccgtgtacag
     1561 tcttcgcccg cctccgcttt ccgccgtggg agaaagcagt ccctccctgt cgtagtattg
     1621 aagcgtgcgg accgtgacgc ccattctttc cgccagcttt cccacggtca tgaagccatc
     1681 cggcagcgcc cgttcatttt ttggcatctc tatcacctcc ctgccattat atttcatgac
     1741 gccgcgtcac aagcaaccct tttttgacgc ccatgcaaaa tattttccgg cgcttggcgc
     1801 aaaaaagcgc cgcgcgaaac ttcgcgcggc gctctgcctg caatcttcat tgaaaagtac
     1861 gcatcagcgt tccggaatca gtacgccgta gctgccgtcc ttgcggcggt acaccgcgtg
     1921 gatctcatcg gtgtccgcgt cgcggaataa aaagaacgtg tggcccagca tattcatctc
     1981 caaaatcgcc tcgtccacgg tgcttggctt gcaggaaaag cgtttttcgc ggatgatgct
     2041 gtatgcttct tccggctccg cctcgtgggc ggcaagcgcg aattcctcca ccgcgggctg
     2101 atagacacgg ttttccaaac gcttgcggtt tttaacgatg cgccgcgtca gcaggtctac
     2161 cgccgcgtcg aacgcatctt ccatacggtc ggccgatttc tcggcacggc tcgtaaagcc
     2221 cttgtcttta acggtcacct ccaccgtctg ccagtccttt tccacggtca cggtaacgtt
     2281 cgcctccgcg ctgtcggaaa agaatttgtc caactttgca agctttgttt ccacgcgctg
     2341 tataaacgcg tccttcaggt tgacttttcg tcctgtgcat gtgatcttca taaaccaaag
     2401 cctcctttaa cagttgaaaa agcggcgtgg acaaccccgc ttcttctgtt ttgattatac
     2461 cacgtcagca cgtgatttta caaggatttt ataggcaata tttacaaaat gttattttaa
     2521 catacataaa aaatatacaa ttcgcacgca atgtaaatta aaaagcggcg caactgaaca
     2581 agccgcgccg ctttgagcgg agatttcttt cattatacgc cggaaaagcc cgcgcgccgc
     2641 aggctctgcc cgctcaatgc ccccggcggg agtaaccgcc gtcgcggcgc gcctcggtga
     2701 cgcggcgcag gtcggagatt ttttcctcgc tgcgggattt gaactggctc atcatgtcct
     2761 caaacgacat ttcaccctgc ggctgcgact gagtgcgggg ctgccaggtg cggccggtat
     2821 ccgcgcgctg ggggcgcggg gggcgcggct cagtgcgctt gatggaaagc gcgattttgc
     2881 cctcgggcga gatgctcatc accttcacct tcacggtctg tccttcgctc aaaacgtcgg
     2941 aaagctcctg gatatactcg ttggacccct cggaaatgtg taccatgccg gtttggccgc
     3001 cgggcaaagc cacgaacgcg ccgaattttt ttacgccggt gatcttgccc tctacgatgc
     3061 ttcctacctc tactgccatg gattgcgata actccttacg ttaaataaag ttataaaaat
     3121 tgcgaagccg caatgcttac caaaaccggt gcagtcagtc ccccgacata tccacataga
     3181 tatgctcgcc gggcagcaca tatcccagct tgtcgcgcgc gatgcgctcc atatacgccg
     3241 cttcgtcgcc ggaatccagt gtgcgctgca gctcggtgtt ggccgcctcc tgggcggcta
     3301 cctgctgcgt caggttctcc agctgctggc gcttggaaac gatgtcgatc tgcgcaaata
     3361 caagcgacac gcacagatag atgcaaacgc aaacaagccc tatgcgtacc gcaagggccg
     3421 aaaagccttt tttctgtttg cgctttctct ctgccataaa cctttctctt ccccggccgg
     3481 cgcgttttgc agcacggccg cacggggatg ctccatccac aaaagggtac acttccccca
     3541 gtgtcacttt gaattataca acacgcggcc gctgttttgc aaccttttct ttctggccgc
     3601 ccggcttttt ttcacacggc cggcgctgtg cgccgtacgc acacgctgaa aacgcgccga
     3661 aaccgcaccc gcaaacgggc gcagcaccgc gcgccacagc attacgaacg gccaggccag
     3721 gcaccagcga acgaacgctt cgacgccgga aagcagaggc tccagtgcgg cgcggctggc
     3781 caaaaaacac agcacacatc ccgccagcgt ataccagcgc ggctggcctg cataaaaacg
     3841 gctcatggcc gcgctgcggt acaaaagcgc ccccacggcc agcacagcgg cgtcgcagat
     3901 aaagcatacc gctttgcctg aacccagcag catgcgtgcg gcgctgtagc ccacacacag
     3961 aagaaacccc agcccggcgg cgtagaacag atcccgcagc gcgagcccca ccgggactcc
     4021 gccaaccatt taccgcacca gccttttaaa cagggattcc tgtttttccc gtttggcggt
     4081 gtactggaca tattcgatgg aaccgttgat cttcacctcg cccgtctgga cggaaagctc
     4141 gctgaccgtc agcccttcac cgccgatggc aagcgtgccg ccgggcgttt ccagcgtggc
     4201 cgtgaaagag tcgtaggaga cgatggacaa aatgcccgtc gctgtaagac gcttgcggtc
     4261 ctctattaca acactgtgcg gcgtctgccg agcctggttc gccgccgggg cggccggatt
     4321 gatcgtcatt tgttttgcac ctctctttat caccatgtgt atgcaaagag cggcgcgcat
     4381 atgacggcgc gcaggaagcc gaagggtctg cgtgcaatcc aaaacggctt actccgaaag
     4441 caccacgtac atctcgcggg caaggtcctt gcctgtgggc gcctcggtgg ataccacctt
     4501 cacgcgcacg ggcctctggc cgaacgccac ctcgatgacg tcgccttcct tgacggcata
     4561 gcttgcctta acgggcttgc cgttcactgt gatgcgcccg gcgtccgcca cctcgttggc
     4621 cacagtgcgg cgtttgatga gacggctcac cttcataaat ttgtcaatgc gcatggctgc
     4681 gctcctttct gttgtcttgt aagaaaaatg cagccggagg gcttgtcctc cggctgcatg
     4741 gaaaagggcc gatatcgctt atttcgccac ggcgtccttc agagccttgc cggccttgaa
     4801 agcgggagcg gtggtagcgg ggatcgtgat ctcctctttc gtgcgggggt tgcggccttt
     4861 gcgggcgggg cgctcgcggg tctcaaacgt gccgaagccg acaagagcaa ccttgtcgcc
     4921 ggccttcaag gcctcggtga tgccgtccag aacagcggcg acagccttct cggcgtcctt
     4981 cttggtcaat tcagcctcag cggcaacttt cgcgatcaga tcaactttgg tcattgtaat
     5041 aaacctccga aataatgatt gagtgtatgt tttcaaagca cggttagcgc tttgtaaaaa
     5101 cctcttttgc tcttttccat agtttgtcca actctgcggc gcttaaatca tccaccgcgc
     5161 agccctgctg ggcggccagg cgttccattt ccctgaagcg ccgcacaaat ttcaccgttg
     5221 cggcgtccag cgcctcttcc gcgtcgcagt ccagaaagcg cgatacgttc gccgcagaaa
     5281 acagcacgtc gcccaattcc tcctgctgcg catccgcact gccctgcgcc acagcctgct
     5341 tcagctccgt cagctcctgt tccaggtccg cgatggcagc ctgcgcgtcg ggataggtga
     5401 agccgtggtc agccgcgcgt ttggccacct ttcggctgcg catcagcgcc ggcagggccg
     5461 cgggcacgct gtccaggtca tcccccgcgc cttcgcggtg cttttcccgg cgcttgagcg
     5521 cctcccaatt ctgcagcgcc tcgtccggcg tctgtgcgcc ggcgtcccca aaaatatgcg
     5581 gatgccggta gatgagcttt ttgcagatgc ccgtacagac gtccccgaac gtgaacgccc
     5641 cctgttcttc ctccatacgc gtgtgcagag ccacctggag caacacatcg cccagctcct
     5701 cgcacagaag gctggcatcg gcaaggtcga tggcgtccgc cacttcgtag gtctcttcga
     5761 taaaattctt gcggatggac gtatgcgtct gcaccttatc ccacgggcag ccattttccg
     5821 gatcccgcag caatgcgaca atctccagca ggtcttccat gttatagcgt tctttctgcg
     5881 tcagcccgtt catggaatac ccctttccgt ctactccata gattttacat gataggccct
     5941 cactttgcaa gtagtttggc cctttttttc ccggatttgc ccccatccgc gggctttttt
     6001 tacatctgcg gccccaaact gcgcggaacc gtttttcttg cgcggacacc atgcgccatg
     6061 ggcgcacgcc aaagggcaga aaagcgcgcc gcgctcccct tttccgggag nnnnnnnnnn
     6121 ccggccccgc tatatttttt tgcgcctgcg gatcaccgca aagcgcattt tcggcccgtt
     6181 acagtttgcc cccgcaccca ggacaaaata tggcatgtgg gtctacctcc gcgccgcact
     6241 gggggcagta gacagccgtc tgtgcatcgt ctgccatggc ggctgctggc gcaggctcca
     6301 gcgccgcgat atccttttcg acagccggga tggtctctat gtagcggtct accagcg
//
LOCUS       DAWBSB010000130         6293 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_2355_length_6293_cov_3.560276, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000130 DAWBSB010000000
VERSION     DAWBSB010000130.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 6293)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6293)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6293
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2355_length_6293_cov_3.560276"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1214 a         1778 c         2087 g         1214 t
ORIGIN      
        1 gctgccagcc gtccacgatc ttctgcttga agatgccgta ttcgtacagg atggaatagc
       61 cagtgccggg gatatcgtcg gtggccatac cgtccagata gcacgcggcc aaacggccca
      121 ggccgccgtt gcccaggccc gcgtccggct cctgctcgta gatattgtcc agcttcatgc
      181 tgtaatcccg cagcacctcg tctgccacgc cgcacaggcc cagattgagc aggctggtct
      241 tcaggctgcg gcccatcaga aattccatgc acaggtaata cacctgcttg ccgttggcgc
      301 cgtaggtgcg cgcgataaag cgcttgcgct tttcgctgag catgccccgt gcaatgagtg
      361 caagcgcgcg gtagatctgt tcgtccgttg ccgacgcgat gtcgatggcg aattcattga
      421 acagcttttc agtcagcagc ttttcaaatt cttttttggt atatctggtg ttcaaaatca
      481 tctactcctc ttttcagggg gaatgaaaaa ccgcgcacat gcagggcgcg gccagacgga
      541 aaaatcatcc cacacgttat atattatagt atttttcggg ttctacttca acaaaatacg
      601 tttttttata aaaagtttca aaaatccact ttctatcttc gggcaattcg tttataatgg
      661 gtataaaaca ggacgtatcg tcaggaaggg ttttcacaca agatgcctgc aaacaagatc
      721 aagcttgaaa tttgcggttc caattacgtc atcgctacga cggataccga ggaatatgtg
      781 ctgtctctgg ctgaaaagct ggattctgat atgacacaga tgctggccag caatcccacg
      841 gcttccgtca cgacggcggc cgtcatcacc gcgctgggtt atctggacga gctgaaaaag
      901 agcaccagcg gtgcggacaa catgcgcgcg cagatcaagg attacctgga ggacgccgcc
      961 aaggccaagc tggctgccga ggaggcccgc cgcgaggtgg agcgcctgcg ccgcgagatc
     1021 ggctatttga aagagaagaa gtaatgccga gccacagcaa ttccacaagg cccgagatac
     1081 tggcgcccgc cggcaatgcc gacatgctct gcgcagccgt ttttgcggga gcggacgccg
     1141 tgtacctcgg gcttcaaaat ttcaatgccc gccgcacggc gggcaatttt accaccgaag
     1201 gcctgcggca ggcggtggct ttttgtcatg cccgggacgt gcgcgtgtat gtaacgctga
     1261 acaccacgct gtatcccggt gagctgacag ctcttgccga ggcggtggcc ggtattgcgg
     1321 ctgccggggc tgacgccgtc atcacgcagg atctggccgt ggccgcgctt gtgcgccgaa
     1381 tggcgccggg cctggcgctg cacggctcca cgcagatgag cgtgcagagc ctggacggtg
     1441 cgcgccgtct ggccgcgctg ggttttacac gcgtgatttt ggcgcgtgag ctgacgctgt
     1501 ccgagatcgc cggcatcact gcgggctgcg gaattgagac cgagaccttc gtccacggcg
     1561 cgctgtgcat gagcgtttcg ggccagtgct atatgagcgc gttcctggga gggcgcagcg
     1621 gcaaccgggg cggatgcgcg gggccgtgcc gtctgccctt cgacgcatcg ggcacgcccg
     1681 ggccggccgc ggggcatcat ctgagtctca aggacatgtc tgtcatcggt catctgccgc
     1741 agctttccgc cgcgggcgtg gccagtgtga agatcgaggg acggctgcgc ccgccggaat
     1801 atgtggccgc ggctgtcaat gcctgtctgc tggcgcgggc gggtgaaccg tatgacacac
     1861 ggcttttaca agatgtattt tcacgttctg gttttacaga tggttattta actggagccc
     1921 ggaacgggag catgttcggc acacgcaccg aaggagacgc cgccgccgcg aaggcggcgg
     1981 cgccgcgcct gcgggagctg taccgccgcg aacgtccccg cgtgggcgtg gggctggagc
     2041 tgactttgga agaggacggc gcgaagctgg ccgtccggga cgccgacggc aaccgtgcgg
     2101 tggtgtacgg ggagcggcag ccccagcccg ggcagaagcc gccggaggag gcgcgggcgg
     2161 cttaccgccg ctcgctggaa aaaatgggcg ggaccccgtt ttttccgcag gaggtcgcgg
     2221 tgcggggcgc acagtggttc ctgccgggca gcgaggtgaa cgagctgcgc cgtcgggcgc
     2281 tggagtccct gctggcaaag cgggagcagc cacgccccat cccgtgcaca aaagtgccgc
     2341 aggctttgct ggaggcgccg gcccgtgctg caaagcagga aggatatgcc gtccggctcg
     2401 cgcatgccgc gcagctgccg cgggagaccc cacaggacgc ggcatggttc attatgccgc
     2461 tggcccagtg gcgcgacgtg ccgccggagc tgaggagcag aacctggctg gaggcgcctc
     2521 gggctttgtt cggcggagcc gaggaggctt cggcgcgcgc cgcgttcgag agccgggacg
     2581 cgggctttgc gggctatgtg gcggaaaata tcgctcagtt taccacgctg gagggcctgc
     2641 cgctggcggg aggcttcggg ttgaatgtga cgaatccgct ctctgcaaaa gagtacgccg
     2701 cgctgggtgc gcggatgctg acgctctcgc cggagctgac gctggaggat atggcgcgca
     2761 tttccgcgcc ggtgcccgtg ctggctcttg cttacgggca tatgcccctg atgctgacgc
     2821 gggcatgccc gctgcagaat gtgcgggact gcggaggctg tgaccgaaag ggcgagctgc
     2881 tggaccggaa gggcatgcgt tttccggtgc ggtgcagcgg ccctgcgggc gcgcgcaccg
     2941 tgtacaatcc catcccgatt tatatggggg accgtctgaa ggagctgccc gtggagctgc
     3001 cgctgctgta tttcaccatc gaaacggcgg cgcgcgtgcg ggaggtgctg gcgctggtgc
     3061 gggagggcct gccttttgac ggtccgttca cccggggcct gtattataag gggacgaatt
     3121 gagcctgtag aaaaagcaag gaggaaaaag cgcccgggga aggccggcgg cgggaaagcc
     3181 gctcatcttt gcaaggcaaa gtgcgtgccg tgcggcaaat gccgaaaaaa tgggcggttg
     3241 ctgcgcccgc aggcgcgtgc cggagcgctg cagagcctcc tgtgcggatg ccgcgccgag
     3301 agcgcggtcc ctttgtgcga agatgctttt ttcattcaaa atgccccgcg gcgttccgca
     3361 gggccgatgc cggggtgtgc agagcgaagc ggcggacgaa gactgccgtg cgcggcgaag
     3421 ggggccgaaa gacctgtttg taaacaactc ggcggaaaag caaagctttt tgacaagctg
     3481 gaattgggag gtttgagata tggttttgaa atacaaaaaa gtgcgtccgg gggcggcatt
     3541 gcccggccgg gccacgcccg gcagcgcggc ggcggacctg cgcgccgtgt gcggcgacga
     3601 aggcgttacc ctgcagccca tggggcgtgc ggtgatccct acgggcatcg ccgtggaact
     3661 tccggggccc gaatatgtgg cgctggtgtt tgcgcgcagc ggccttgccg taaaaaacgg
     3721 gctgacgctg tccaacggcg taggtgtcat tgattccgac taccggggcg agatctcggt
     3781 ggggctggtg aatctttcgg acaagcccta tacggtacag aacggcgagc gcatcgccca
     3841 gcttgcggtg atgcccgttg ccttgcccga catgctggag gttgaaacgc tcaccgacac
     3901 agagcgcggc acgggcggct tcggctccac aggacggcag tgaaaaggag ggggaaccat
     3961 gaaactcaag cgtaatctgc ttttgatctt ttttattctt gcgggaatcg tgctgggcgg
     4021 aatgcttgcc aacctgtgcg ccaatatccc gttcctcagc tggctggcgt attccagctc
     4081 cattggcttc aacgccgaca gcccatttgt gctggatctc tcggtcatca agctgacgtt
     4141 cggcttttcc atgggcatca gcgtggcgca gatcatcacc atcgcgctgg ccattttctg
     4201 ctacaacaaa acgaacctgc gctgagctgc ggcccggcgc ttttacggaa agaagggacc
     4261 tgacatggga attatcctgg cttcatcgtc gccccgccgc agggagctga tggcgcttat
     4321 cacaccggat tacacggtgc gcaccagcga cgtggacgaa cgggccatcc gtgccgaaac
     4381 gcccgcgctt ctggcacaga agctggccgc tgccaaatgc cgcgccgtgg cggagagctg
     4441 ccctgaggat gtggtcatcg gctgcgatac ggtggtggac gtggacggcg cggttttcgg
     4501 caagcctgcg gaccgggacg acgcgcggcg catgatacgc gttctggcgg gcaggggcca
     4561 cctggtgcac acaggcgtgt gcattgccgc acagggacgg gagctgtgct tcgccgcaac
     4621 cacaaaagtg gtgttcggcg cgctttcgga cgcggagatc gaggcgtaca ttgcgacaga
     4681 cgacccctac gacaaggccg gggcctacgg cgtgcagaac ggcgcggcca aatttgtggc
     4741 gggtatcgag ggctgctatt tcaatgtgat gggcttcccg gtgtcccgtg tgtacaatgc
     4801 gctgaaagag ctggacgtgc tttgatctgc ctgaaccagc ctgcacaaaa ttttacaaat
     4861 ggacgcgcgg caggcaaaaa agggcggcgc aacagaaaag ccggcgggag attggctttt
     4921 ttctgtgaat gtgccaaaaa attttgttgt acaaacagct tttttgtgat atactgagga
     4981 gcagagtggg gtataggccc tgcgtgtgaa ttttagggaa agggacgaca tgggcgcgaa
     5041 tgtgatcatg gtcgcctcgg gcaaaggcgg caccggcaaa agcacggtag ctgtgctgct
     5101 gggggccgag cttgccgcgc gcggcaggcg tgttttgctt gtggagctgg attccggcct
     5161 gcgcagcgtg gattatatcg cgggcgtata cggcaagact gtgtacgacg tggaggacgt
     5221 gctgaacggc cgctgcgaag ccggcaaggc cgtggtggaa agccctgtgt accgcaacct
     5281 gtatgtgatc tcggctccgt actccggagg acatatcctg ccggcagcgc tgcgcgtgtt
     5341 tgtagaaaag gtcggcccgg tgttcgacac cattgtgctg gacactgcgg cgggcatggg
     5401 cgttccgttt gaggcggcga tgggcgtggc ccaccgcgcg ctgcttgtac ttacgccgga
     5461 ccctgtatcc atccgcgacg gacgcattgt gtgcgacgcg ctggaggcgg gcgggtgccc
     5521 ggaaatacgt cttatcatca acaaggtgcc gcgcacgctg gcaaactgcg gcatacaaag
     5581 cctggacgaa tgcatcgaca ccgtgggcgc acagctcatc ggggtggtgc cgttcagcac
     5641 cgaaatacaa aaagcgggcg cgacaggcgc gccgctggct ccggcgggca ggccccgtct
     5701 ggcgctgcgc gccattgccg cacgcctgtg cggacaatat acgccgctcg tgattcgata
     5761 aatatgagga ggatgcccaa atgaacatcg cgttgatcgc gcacgattcc aagaaggaac
     5821 tgatggtgca gttctgtatt gcatactgcc gcgtgctttc ccagcacgat ttgtgcgcga
     5881 caggtacgac gggcaagctt gtggcggagg ccacgggcct gcgcatccag cgttttctgt
     5941 ccggcaccca tgggggcgac cagcagatcg ctgcgcgcat cgcctgcaac gagatcgacc
     6001 tgctgctctt cttcagagac ccgatcagcg cgaagcccca tgagcccaat gaaatgaatc
     6061 tgctgcgcct gtgcgacgta cacaatatcc ccatcgcgac caatatcgcc acagccgagg
     6121 tactcatcca tggcctggag cgcggcgatc tggactggcg caatatcgtc aaccccaacc
     6181 actgaacgca tgcaactgca aatgccccgt ctataggacg gggcattttt gtatgtcgag
     6241 gtgaattata ctatcaagtg caacacttta ggggaacaaa aagaagtcca tac
//
LOCUS       DAWBSB010000131         6292 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_2357_length_6292_cov_2.759500, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000131 DAWBSB010000000
VERSION     DAWBSB010000131.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 6292)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6292)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6292
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2357_length_6292_cov_2.759500"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    938..947
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2885..2917
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5138..5147
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1312 a         2043 c         1620 g         1264 t
ORIGIN      
        1 catcacgccg ttcagcgtca cagcgcctgt ctcgaaattg atatcaaccg tatcgccgcc
       61 cagagcgatg atgcgcttga ccaacgggtc tccgtaattg atgtagccgt ccaccaccac
      121 cacatcgccg cgctggggcg tatacagcat gctctgtacc gccacgcggt ccccgccgta
      181 aagtgtaggc tgcatggaaa cgccggaaac ggtgatgatg cgcacggcaa acacaaaaaa
      241 cagtacgatc accgccagcg cgaacaccag tgaatcatac cactcgaaca gcgcgttttc
      301 cttagactgt ttcggttcca tgcacacacc ttcttttcat caataatata acaaaaaagg
      361 gacaatgtac attgtccctt tttgtgaact tatcgaagct gttctttgac cttggcggcc
      421 ttgccggtgc gttcacgcag atagaagagc ttcgcgcggc gtacacggcc cttgcgcaca
      481 acttccactt tttcaacgaa cggagagttc acggggaaca cacgctcgat gcccacgccg
      541 taagcagtgc ggcgcacagt aaaggtctcg gcaacaccgc cgtgcttctt cgcgatgaca
      601 gtgccttcaa acgcctggat acgctcacgc tcgccttctt tgatacgaac ggaaacacgc
      661 acggtatcgc ccacgttgaa ctcgggacga tcctctttga tcatgccttc ggtaatgatt
      721 ttcattgcgt ccattgttgt acaatcctcc atgtttattt agacattcat acccggcgga
      781 gcgccggcag aggactgtcc gtttaatgtg tgacattaac cccgctgttg gcacagtgga
      841 tataacgcct aaatataata ccacatggca attccgaatg caagcatttc aggaaaattt
      901 ttcgccgttt cgggtgtaga tcccggtgcg caggatannn nnnnnnngga tatctcccga
      961 tgcggcgggc aggccgtgct gctgctccag ccaccccagc accagaaccg ggtttacatt
     1021 cagcacactg cccgcaggca aacgcataca caccacaagg ccgccgtttt tccgcataag
     1081 ctgtacctcc ggcaaatgcg ctttcagatc cactgttttt ttctttccgc gcttgccctc
     1141 cttcactacc tcggccgcgg gcgccgcgtt ataggcctcc catgctgcgc atgctgcctg
     1201 cgcgtctttt tccgcgtagc gcacctcata agcggcgcac gcaatatcgc ccgcatccag
     1261 cacggcgggc gcaatgcgcg tcaccttaat gccccggggc aggcacgcag aaagcgccgg
     1321 agccgcggcg tcccagtcgt acccctccgc ctcggacttg aagtccaccg tctcggccaa
     1381 gctctcctgc cccagagaca gcggcgcggc aaacgtcata taaatgtgag ggttgaaccc
     1441 cagtgtatac cagacaggca agcccgaacg cttgagcgcg cgctgcatca cccgctgcag
     1501 atccagcagc gaaatataag acgcctctcc cgttttggtg aaccagactc taatcgtagt
     1561 caaaacaggc acctcccagc agcttatttg cgccgcagcc gctgcacgcg cggttgcacg
     1621 gcggcgtggt ctgcgcgccc agcgcctttt ggtattcccg caccaggtac tccctgctca
     1681 cgccgtagtc caggtggctc cagggcgtaa cctcgtccag accgatggtg cggttcgcgt
     1741 aaaaagcagt atcaaagcca aggtcgcgaa acacctccag ccatgtatcg taacgaaact
     1801 gctcgtccca gccatcaaag cagcagcccc ggcggtaagc ctccaccaga acccgggaga
     1861 gccgccggtc gcccttcgca aagaccgcct ccagaaaact ggtatgactg tcgtggtagc
     1921 tcacactgat cttgcggctt ttgacgctgc gcagcagatg ctgctgtttt tcacgcagct
     1981 catcctcggt attctggccc acgaactgga acggcgtcat aggcttcggc acaaaacacg
     2041 ccacgctgat ggagacctgc acgccgcggc ccttgggttt gttttcgttt ttataataga
     2101 ggtccacaat gcgctgcgcc gtgtcggcaa ttccctcaac gtcctccatc gtctctgtgg
     2161 gcaggcccag catgaagtag agcttcacgg aggtgtagcc ggcgctgaag gcaaggttgc
     2221 aggtgcgctc gatttcttcc tcggtaacgt ttttattgat gacgtcccgc agacgctgtg
     2281 ttccggcttc aggcgcaaag gtaaggccgc ttttccgcac cctggtggtt ttgcgaacca
     2341 gagactccga aaagttgtcg atgcgcagcg acggcagcga catactcaca tgctctttgg
     2401 gcgtccattc cagcatatcg tccagcagtg tttccagttg gctgtgatca ctggtggaaa
     2461 gcgacgtcag agagatctca tcgtaacctg tattcccgca caggctgcgc cccgccttgg
     2521 agagcatatc cgcatcccgc tcacgcatgg gacgatagat gaagcccgcc tgacaaaaac
     2581 ggcagccccg cacgcagccg cgcagcactt cgatctgcgc gcggtcgtgc accgcaccga
     2641 tcatcggcac cacaaaattc tccggcagcg gctggctgtt catatcctga atgatggcct
     2701 tgcgcaccac ggccggcgcg ccttctttcg ccgtgatggc ttccacgcgg ccgtcgggcc
     2761 gataggacac atcgtaaaac gcgggcacgt aaacgccttc gatatggcac aggcgcttta
     2821 atatctcccg tttggtgagc ccctctctgc gcccctgaat gatggtgtcg cacacatcag
     2881 gcagnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaaa acggaatgtg cgccaggtcg
     2941 agcatggcaa gaatattggt gtaggaaagt tcatactgaa gtgtaaagcc cactacgtcg
     3001 aacatcccca gcgggtcttt gctctccagc gcatacagcg gaatatcccg ctgctgcatc
     3061 tgctccttca tgtccaccca aggcatgaac acacgttcac accagatgtt gtcccgtttg
     3121 ttgaggatct catacaatat tttcatgccc agaaaggaca tgcccacctc gtaggtatcc
     3181 ggaaagcaga aagcgaaccg aaggtccagc ttctccttct ccttatatac gcagcccggt
     3241 tcgccgccgg tatagcggcc cggtttctgc actgcggcaa gcgcagcttt cagcttttca
     3301 tccaagcgtt caaacatcct ttcccttttt cgggcttata cccccgcgtt gtttcaccat
     3361 accccaagct cacacttcac gttccgcttg gcattcactt aaaaaacatg acatctatgc
     3421 cgccaaactc acgccctgcc cgcggcccgc attcactaag cgggcatatt ataccatgac
     3481 gccgcgaagc ggcctcaaca aaaccgcatc tgctgcggtt ttgcatggtt caatgttctc
     3541 aaagctctgt gcgccagcac agggcgtgtg ccgcgcacac gcccggccgc gcaagcggca
     3601 gttttggggt cattataccg tatttgcgcg cagaatgcaa acagaaaagg cctgtgcacg
     3661 gtgccattca cgcgctgcgc aggcctttct tctttatttt tgcctgaact cctttgccag
     3721 cagataaccc ggccccagga aagccgccgc acgagcgccg cgcggcatcc cgcccaacag
     3781 cgcaaagccg agcgccgccg cgcagaacag gcacaacagc agccgctggg cacggcgcag
     3841 cgcatcctgc ttttccgcag gcagcaaatt ctgcagcagg cgcgcgccgt ctaacacgcc
     3901 gaacggcatc aggttgtaaa cgcccgtaca caaactgacg gcggcgagaa aatacagtgc
     3961 gtagctcgcc tgccggtgaa gctgcaaaaa aagcagcgcc gccatgataa aattggccag
     4021 cggccccgca gccagcaccc atagctcttg tgtgcggagc aactgctccg ctcccgaaag
     4081 cccgatgcct cccgcgctga aatggagacc gggcaattta tgcgtacaca gcatatatgc
     4141 cagcacatgc ccgctttcat gaacgaaagc gcagaccagt gtcatgcgaa gaaagcccat
     4201 agggtcgaac aaaagccccg ccgccagaca ggcgcacagc agtacaggct gcaccgacgg
     4261 cccgctcatg cggccagctg cggaaaactg ggcatcgggt ccacccgcac gccgtcgaga
     4321 atgatctcca gatgcaggtg cgggccggtc acaaggcctg tgcttcccac cgctgcaaca
     4381 gtctgcccct gcgtgaccgt ctcccctccg cgcacaaaga tgtaagagag atgctggtac
     4441 agcgtctgga cactgccctc atggcggata atgatataat agccccgcag acggttgtag
     4501 cccgtgcgca ccacctggcc ggactgtgcc gctgccacag gcgtgccctc tgccgccgcg
     4561 atgtcgacac ccgcatggaa atcatccgca ccgttcaccg ggttatcccg gaaaccgtac
     4621 cccgaagtga ccggccccgc caccggcagc agaagctcct gcggcagcgt ataatcgtcc
     4681 atggaagcgc cgtcaggcac ctcacgtttt tcgataccgg gccaaaagcc gccctgcccg
     4741 tccaacgcct ccggatcttc caactgccgg accagccgct ccgcgcccat acgcagatcc
     4801 tccaccacgc cgtttgcaaa ccgcgcaaat gcgttatccg tggaaaattc cacgcccgct
     4861 gtcatcatct cgtgatactc agccctcatg tcctccagaa aaggcgcgcc ggcgctgcgc
     4921 gcaaaaaaca caaacgccac caacagcgcg cacagcagga cctgcacgaa caggacatgc
     4981 tcttcgcccg agtttacctg aggcaccgta aacggctccg gcttcacctt tgctgtgggg
     5041 tagagctgtt ccaccggcgc tgttcccgcg tcccgctgcc cctccggcat gtcctcccgg
     5101 tctcccgcag ccgccgaaaa cgttcccgga accggctnnn nnnnnnngct caaaccgctc
     5161 ggcccgttcc ccctgcggcc gctgtgtccg gcgcgccttt ccgtcccatc ctgcttctgg
     5221 tgtgccaatt tcctccggag ggtcctcatt cagccgcatc cattcctcct gccgcccgct
     5281 gtccttttcc cacatcaacg tcacgcacct ttttatcccg gtttttcgga acgctcattc
     5341 ctttaaaaag gctatgcggc gaacgccaaa aaaagaaccc ggacagcctg tgtacaacag
     5401 attgtccggg ccggtaattt gggagggtca gcgtcccagc aggttcctga gcctgcccag
     5461 cagcgtttgc gggcgcggcg ctgccgggcg gcgctgaggc tgctgcaaag cggcagcatg
     5521 gcggcgtgct gtcggcgcac ggcgtaccgg ctcccccgcc gggcgcggcg caggcatggc
     5581 gggccacgtg ccggccagca tatcatacat gccgagctgg ctgtcaacgc ggggcgcgtc
     5641 cggcgcaggc ttcaccttgg gataggtgcc gtccggctgc atttcccggg cattcacatt
     5701 gtcgcgcagc tgcagatcca gcatctgcag cagctcaccc gcaatgcgtc tgtcctgaat
     5761 acgcacgccc acctcgacac gtcgttcggt gttgcgggta agaaaatcgc ccgacgcgat
     5821 atacacccgt gtatcctgcc cgcagccaaa agcatacaca cgctcgtgct ccagatagcg
     5881 gcccacgatg ctgcgcaccg tgacattttc cgtcaggccg ggtaccccgg ctttgatgca
     5941 gcagatgccg cgcacgatca tctgcacaga tacccccgcg caggacgctt cggatatttt
     6001 ttcaatgata tccttatcgc tgatggaatt acacttaagg atgatgcgtg cgtccttccc
     6061 catgcgtttc gcctcgatct ccgcgtccat ctcctgcatc agcacgcttt tgaagcacag
     6121 cggcgcgacc agaaggttgc ctgcgtgctc tgtcaggcgt tcaatggcaa gattattgaa
     6181 cacggttgcc agctcggagc cgatgccgag atcggtcgtg ataaacgaaa ggtccgtata
     6241 ctgctcggag gtcttttcat tgtaattgcc tgtgccgatc tgggcaatat ag
//
LOCUS       DAWBSB010000132         6261 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_2365_length_6261_cov_3.248791, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000132 DAWBSB010000000
VERSION     DAWBSB010000132.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 6261)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6261)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6261
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2365_length_6261_cov_3.248791"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3593..3602
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4041..4050
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1248 a         2036 c         1732 g         1225 t
ORIGIN      
        1 gggctgggcc tgggcaaaaa gctgggcatc cggttctacg acaaagagat cctgaaaaag
       61 gcggccgagg agagcggcat cgtagaagag ctgtttgaaa aggcggacga aaagcccacc
      121 aacagctttt tgtactccct gtccatgggt acgcacggac aggccatgaa tttcaccaat
      181 tacaacgatt acctcaccaa cgacaagctg ttcctgttcc agagcaatac catccgcgat
      241 atggctgaaa aggattcgtg cgtcatcatc ggccgttgtg cggattatat cctgcgcgga
      301 cgcaaggaca tgctttcagt gttcatccac gcccctatgg agctgcgcat ccagcgtatc
      361 tcccgcgtgc gcaatgtaga agaagacgcc gcgcggtccc tcatcaaaaa gacggaccgt
      421 cagcgcgcca attactacaa cttctacgcg gacaacgact ggggcgcggc cgacagctac
      481 gacgtggcca tcaactccgg caagctgggc gtggaaaaat ccattgaggt tctcgccggt
      541 atttgtgaac acctgtaaat tgctgcaaaa accgcatggc aaaagccatg cggtttttga
      601 tttctccgca ataaaaaagt ttatcccgat ggctgcgggc cggggacggc ccgcagaaaa
      661 actttaccgt tcaccgaagc cggatgtgca gcgtgccgtc gctgtacgca aagcggcccg
      721 cgggaaccag gcgggcgggc gctttgggcg cgcacgttcc ctgcggctcg cgcagcacat
      781 cgtccagccg gaaggccgag agtaaaagcc ctgcaagccc cgcctgcatt acacccgtcc
      841 agccgccgtc taaaaagggc agcatccctc tcgcggaact ccagcccaga caggagagca
      901 ggttgagcac gccgtgcatc gtgagcagtg cacagcagcc caccgcgcac agccgcccga
      961 tgccgctgcg ggtgcgcagg gccgtgcgcc acagccatgc cagcgcgccc gcgacagctg
     1021 cggccagcgc tgcggcgcag gcaataccgt accgccagcc cgcgtaagcc agcaggtaat
     1081 cgacgcgcag ctcccgcacc gccttccctg caaattctcc cacggtgccg agcgccgctt
     1141 tttccccgag cggcaaactg ccgtctccgg ggccaagcca ccgcatcccg tcccacatgc
     1201 gccgcagggc gttcatccaa tagcccgcgc ccgctgcatt tgcatccggt gcaagcagca
     1261 cctccagatg ccgctgaagg ctcccggcca gacccggcat caacagcagc accagcaaaa
     1321 atgtcaccac aagagagccc gctgtcacgc ccagcacaac cgcaaagctt ttctttccgc
     1381 caagcccata ccaatcgcct gcgcagcata ccagacacac ggcaagcccc gcaagcccca
     1441 gcacacacgc cgcggccaga tgcggcacca tgatacacag ccagagcccc ggcacaatgg
     1501 caaaaccgct gaccagcagc cccgcccagc cgcggccgcg ctgccggtac accacggccg
     1561 cgtaaatggg aataaaaatc atgctgaaat gagtggcgtc gtaagataca ccccaaacag
     1621 tccaaagacg ccgcccgttc atcagggtat atccggccag aaaaccataa gccgaaacgg
     1681 ccgccagccc gatatagagc gcctttgtcc agcgtgccaa cagggacaca tccatacaga
     1741 aatataccgc cgcaagcaca gcaattcccg ccaaggcggc ggccaggctt tggtacgcgc
     1801 cctcgcgcca caggtacacg ccttgtgccg aaacgggcac gctccagaaa aaacgcagca
     1861 gcgcacccgc cagcaacagc gccgccacca taaaaaacgg cccccaggcc gggcggggcc
     1921 tgtgcacgcg gtccagcgcg ccgcctgcca gcaccgggtc tcccatgctc tccaccgtcc
     1981 tgcgctcggc ttcttccggc gcgcagcctt gggccacaaa ggcgttatac tgttcgctga
     2041 gatgattctc cagctcccgc tccacacccg gcaccgcacg gcggcagcgc agctgggctg
     2101 tgacggtctg cagccattcc tttcgcgtct gtcccattac agcaccaccc ctgcgcgagc
     2161 atccattgtt tccaacacag cgttgatcgc ccgggacacc cggcgccact ccgtttgctg
     2221 cgcctccagc gcaccacgcc cggccctggt gatacggtag tagcgccgcg gcttgcctgc
     2281 tgtggccgcg ccgcccggct gttcatagct ttcaatgctg ccgtccgcct ccagcgcatg
     2341 cagaatggga tacagcgtcc ccgccttcag cgcgaatgta tcgttggagc gctgtgccag
     2401 ctcctcgatc atctgatacc catacatgtc ccgctccgcc agcaggcgca gcagcagcat
     2461 ctgggccgag cccggcacaa ggttttttcc cgccgccatg taaattcctc ccttttgccc
     2521 cgtgctgtgc acggcggtca tttatataga ttatctatat attctattgt ttagtatata
     2581 tctatgccct atatatgtca atggttttca aaaatttttc caagcacaaa aacgcccccg
     2641 ctgcaaaagc agcgggggcg tttgaaaaca gagggaatta tttggaaagg cgctcgcgca
     2701 gggcagcagc ctcggcgtgc agctcggcgg gaacatggtc gccgatttgg gcgtacagtt
     2761 cctcaacacc cttcacgtct tccagccaca gctccttatc cacatccagc aggcccttga
     2821 tggtgtcaac ggtgacgccg tccagaccgg taatgtcgat atcctcgggc ttgggctcgt
     2881 agccgatggc cgtctcaaca gcctcagcct cgcccttgca gcgcttcaga atccacatca
     2941 gcacgcgcat gttgtcgccg aagcccggcc aaataaagtg gccttcgtcg tcggtgcgga
     3001 accagttgac gttgaagatc ttcggcgcct tgtcgcccag cttcttgccc atctccagcc
     3061 agtgcgcgaa gtaatcgccc acattgtagc cgaagaacgg acgcatggcc atcggatcgc
     3121 ggcggacaac gcccacagcg ccggcagcgg cggcagttgt ctcagaagcc atggcagagc
     3181 cgacatacac gccgtgcgcc cagctgaagg actggtagac cagcggagcc gtcttggcgc
     3241 ggcggccgcc gaacaccatc gcggtgacag gtacgccgtt ggggttgttg aactccttgg
     3301 acaggcaggg gcagttcgcg gccggagcgg tgaagcggct gttggggtgg gccagcttgc
     3361 cgccgtttgc aatgtactcc ttgccgtcca cggccaggcc cgtccactcc tgaacatcct
     3421 caggcgggtt cttgtccaga ccttcccacc acacggtgtt gttctccagg ttatgcgcaa
     3481 cgttggtgaa aatggtgccc ttcatcgtgg tgtgcagggc gttggggttg gatttctcgt
     3541 tggtgccggg agccacgccg aagaagccgt tctccgggtt gatggcatac agnnnnnnnn
     3601 nngtccacac cttgtagccc tgcttggcat acacttcggg cgggatgagc atggccaggt
     3661 tggttttgcc gcaggcagag gggaacgcgg cggtgatgta gtccacgttg ccctgcgggt
     3721 cctcgatgcc gaggatcagc atgtgctcgg ccatccagcc ttccttgtag ccctggtagc
     3781 tggcgatgcg cagtgcgaag cattttttgc ccagcagcac gtttccgccg tagccggagt
     3841 tgatggacca gatggcgttg tcctcgggga actggacgat gtagcggttc tccgggtcga
     3901 tgtccgcacg ggcatgctgg ccgcgcacga aatcgttgga atcgcccagc tgacgcaggg
     3961 ccttcacacc cacgcgggtc atgatatcca tgttcagcac aacgtagatg gagtcggtca
     4021 cttcaacgcc gaccttggag nnnnnnnnnn tcgttggggt ccatccagtt gaccgtcata
     4081 cagtcgtccg ccttgttgga ggtgcagatg aacgtgcggt cctccacgcg ggccacgtcg
     4141 tttttcgcgg tatggtgcag cacgcagccg ggcagctcct cctggttcag ctccgtcagc
     4201 tcgcccgtgg cgaacgcctg ctcacgcagg gcgcgcagct ggccttccga accgtcgatc
     4261 cagatggttt tgtccggggt catgagctct tccatctctt tgacccagtc cagaacatgc
     4321 tggttgcttg tcaatgccat aatcaaatca cctttcttta atgtaattcc ctctgttttg
     4381 aaacgcgcgc ccatggcccg cgcttccttt cgcatcgcgg ggcctgcccc cgctttccaa
     4441 taaaatgcga tttggattta ttatacctct ttgttaaaaa aagctcaagc tgttttacgg
     4501 aaaatgagcc gaaaagacaa aagatttaca tcgtattcac agatttcccg tagggattgc
     4561 acgatcgtgc aatccgggcc gcggacgcgt tccgcacagc ggcacgcgcg gggaagcgcc
     4621 gcgttcattc aaaaacgggg cgcccgcgcc gcggcctttc gccgcgccgg agcgccctgc
     4681 ccttatgccg tcacagcggc ggcatcatat ctcataccat ttgatctcgt tggccgccat
     4741 atccagcgca aagctggagc cgtcgccctt gtaaacaacg gtgctctgcg gctcgtaggt
     4801 gttgttcaca acgcagtact tgccgttctt cacataggcg tgtacctcca cgttgaagtt
     4861 gctgctgaac catttgtgca ggctttcttc gtcgtgggcg ctccacagga tgctgcggta
     4921 caaaacgcgg ctgttttcaa agctgtacgg caggccgctg atgtacaccg cccggccttt
     4981 gccaaacgcg tgcacggcca tctgcacctc tttttcccgc tgcaccagga tctccgtgtc
     5041 cggcagcgcg tagatgccct tttttccctc gccgaaatcc accggcttcg tgcagtcctc
     5101 cagaatgaag tggccgggat gctcgtccca gttgtatttg tcgtagccca gcgtgaagcc
     5161 cgtttccttc tccacgccca gtacgccggc cagctgcaga taatgtccct cgtactggtg
     5221 gcccgatggt tcgcccacgc cgacgatgcc gccgccattg tacacgaagc cgcgcacagc
     5281 ggccgccaca gccgggtcct cccattcgcc gccgccggtg tgggccgtgt cgccgtcgcc
     5341 cacattgatg agcacgtcca cgccgtccag cacatgggcg tccgcgcgca ggtcgtcaaa
     5401 gctgatgaac cgcacgtcga acggcgcgcc cgaaagcgcc tcaattacgc ccgcatagct
     5461 gtaattctgc ttctggtaca gcgcgtggtg caccatatgg cagccccagg cgcgcatttt
     5521 gccccagcag ttcagcaccg ccacggtttt tacgcagtat ggcgtcgtgc ccttgatgtt
     5581 gtcgtacagc tcgcggaact cattgcacac gctctccaca taatcgatga attccggaaa
     5641 cgcacaggcc agcttcaggt atccgccgta gccgatgcgg tcgatgggct tgcgcaggat
     5701 ggcgcgccgc gctgtcaccc agttttcctt ggcctcccgc acagggtcgc cgccctcgtg
     5761 gaaggtgtcc gggaagaagt aaggcaaaaa gcggccctcg gtatatttca cacccggaat
     5821 atcgctgata aggcgcagcg tgctgccatt gcccacgctg cccaccacgg catccaggcc
     5881 aatgcttttg aactcctcca tgaacggctc ggtgccgatc cagtggtcgc ccagaaacat
     5941 catggcttcc ttgcccagct cgtgggtgac gtccaccatt tccctggcca gttttgccac
     6001 ctcgcgccgc tggaacgcct ggaagtcctt gaattctttc gagggcacgc ggtactggtt
     6061 gttgtaataa ccctggtcga tgatgaattc gggccggaat ttgtagccag cctcccgctc
     6121 gaactgctcc agaatatacg gagacacgct ggcggaataa ccgtaccagt ccacgtattt
     6181 ttcccgctgc agctcatcga acaccagcgt gaactggtgg aagaaggtgg tgtagcggat
     6241 gacgttcaca tacgggtgct c
//
LOCUS       DAWBSB010000133         6239 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_2373_length_6239_cov_2.739812, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000133 DAWBSB010000000
VERSION     DAWBSB010000133.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 6239)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6239)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6239
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2373_length_6239_cov_2.739812"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    665..674
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1117 a         2015 c         1750 g         1347 t
ORIGIN      
        1 gccgggcttt tctctgcttt tccaccggac agaccggata cttattcttt cggcgcttct
       61 tcggcagaag gcgtttcgct ctgcacagag gcaggctcct gcacagccgg ttccttcacg
      121 tgcacagaaa ccctgcggcg cggtgcggag cttttttcgt cctccgcctt ttcggcgtct
      181 gcgcggggcg cgtcgtcctt gtccaagggc ggcagtgtgc cgccttccat cagtatgcgg
      241 aattcctcac cggaaagctt ctcacgctcc atcagcgctt tggccacggt gtgcagctta
      301 tcgatgtgct cgctgagcat cacacgcgcc gcctcatagg cctcgtccag catctcgcgg
      361 atctcggcgt ctatctcaga agcaactgtt tcggaataac cgcggcctgc actcaggtct
      421 cggcccagga acgtctcgct cggctcgttc ccatatacga cgggtcccag ccgctctgaa
      481 aagccataac gcatcaccat gctgcgcgca atggccgtgg cgcgctccaa atcgttggac
      541 gcgcctgtgg agatatcgtc cagaaccagc ttctcggcca cgcgtccgcc cagcagcgta
      601 acgatattgt cttccatctg ctttttggta acgtagttct tatcctcttc aggcaggctc
      661 atggnnnnnn nnnnaatttc atgcacgggg tctgccgtct cgcagtaata atgcgtgatg
      721 gcatgccctg cttcgtggta ggatgtcagg cgcttttccc gctcggtaac gacgcggctc
      781 tttttttcgg ggccggccac caccttgatg ctggcttcct cgatgtcctg ctccgtgatg
      841 gctttgcgcc cgcgcttcgc tgcaagcagc gccgcctcgt tgacaaggtt ctccaggtct
      901 gcgcctgtga acccggctgt tgatttcgca atggttttga gcaccacgtc cggcgcgagc
      961 ggcttgttgc gcgtgtgcac cttgaggatc tcctcacggc ctttaatgtc aggcaggccc
     1021 acatatacct ggcggtcaaa ccggcccggg cgcagcagcg ccttatccag gatatccgcg
     1081 cggtttgtgg ccgcgataac gataacgccc tcgtttgcac cgaagccgtc catctccacc
     1141 agcagctggt tcagcgtctg ctcacgctcg tcgtgtccgc cgcccagccc tgcgccgcgc
     1201 tggcggccca ctgcgtcgat ctcatcaatg aacacgatac agggcgcagt ctttttcgcc
     1261 ttatcgaaaa ggtcacgcac acgggacgcg cccacgccca catacatctc cacaaagtca
     1321 gagccggaga tggaatagaa cggcacgccc gcctcgcccg cacaggcgcg ggccagaagt
     1381 gttttaccag tgcccggagg gcccaccagc agcacgccgt gcgggatacg cgcgcccagc
     1441 gtgttgaatt tcgtctgatt tttgaggaac tcgacaatct cctgtagctc ttctttttcc
     1501 tcgtctgcgc cggccacatc cttaaatgtg gccttacggc cctgctcggc ctggtccttt
     1561 accttggccc ggcctacatt catcgcgccg cccccgccgg actgcttgta catgaaatag
     1621 tagaagcctg ccatcagcag aacggagatc agcagcatcg gcagcatcgt cagccagacc
     1681 ggcgtttccg tggcgggcag caggtccatc ttcagttccg caccgccatt ttttttggca
     1741 tagatttcgc ggtagcgctg caaattatcc ttgtccagca gttcgcccgc atacggcagc
     1801 gtataggtga gcacagcctg ttttgccgtg tccgaagcgg cgcccgatga cgaaccgccc
     1861 cacaggccgc ccagagcgcc cgtttgctgg ccgccgagag tcatgcccgg ctgctgctcc
     1921 agcagctcct gcgtggcctc ctccgtcagc gtaagctgca tggccccgtt tttcatattg
     1981 agcacgaact cggacacttt gccctgttca aaataatcaa tgacctctga atatttcatc
     2041 gtctgggtat cgccgcctgt gctgaacagg atgaacgcca tgatgatgag gcccagcagg
     2101 gcgatgatca tcagcccgct tgtgcttttc cggtttttat tcaaatcagt ttcactcctg
     2161 aaggaggctt cttcggcgcg cctgccttaa atacgctcgc accgccgcgg tgcgccgggc
     2221 cgttcgctta tacggaatag acctcgggtt tcagaatgcc gatgtacggc agattgcggt
     2281 atttttcatc gtaatccagc ccgtagccga tgacgaactc gtccggcacc gagaagccga
     2341 tgtaatccgc ctgcaggtca accttgcggc ggctgggttt atccagcagc gtggcgatgc
     2401 ggatggagcg cggccccctg ctctccagca gctccttgat atagctgagc gtgaggccgg
     2461 agtccaggat atcctcaacg atcagaatat ccttgcctgc aagggggacg tccagatcct
     2521 ttacgatctt cacgacaccg ctggatttga ccccggagcc atagctggac acaaccatga
     2581 aatcgatctc ggcaggcacg tcgatctgac gcatcagatc cgccagaaac accacggcgc
     2641 ccttcagcac cgtcaccagc atcaggttgc tgtttttgta atccgccgtg atcttgccgc
     2701 ccagctcccg caccttttcg cggatctcat cctcgctgag caggacgcgc aggacgtcct
     2761 ctttcatatt atcgttcatg ttacttcctc catctgccgt gccgtgaccg tcacagcggt
     2821 ttttgtaaat tcatccgggg ccaggccttc ggcaaaaccg aagcccgccg cccataagac
     2881 tgtgcctccg ctggcgagca ccggcagggc gtcacgcgcc gcggaaggca ccctttgttc
     2941 cgaaaacagc tttttcaacg gttttgtcac accgcgtccc gcggggcgga acatatcgcc
     3001 cgggcggcgt gtacggaaac acggattatc ctgtatttta ccataatccg cgcaaaattt
     3061 taagaggttt ctcacatctt tctcagaatt aaccattttt tcataattga ccagctgaac
     3121 ctctaccaaa agcccgcccg gcgcggcaaa aacacccggc gcaaacggct gccgccagct
     3181 tccggcctct ccgcgcggcg gttccggccg gggacctacg gccagcaggc cctgccgtat
     3241 ggaaagccat acgccctcac agagctgcac cgcaccctcg ccttttgcca aaagcgcgtc
     3301 cgcctcggga acaaggctca ttttctgctg cggcgctgcg gccgcgatga gccgggccag
     3361 cgccgtatgc cgcacggccg cgggcgccgc agccagcgtc tgcgcacagt aagcgtccgg
     3421 gatatttccc ggcaaatgca cagctcgggg cacgcacgcc tgttccagca gcgctccagc
     3481 ctgttcctcc agataatccg ccaccgcgga catctcggcg gcaaaacgcg ttatgctgcg
     3541 cgcggcgccc ggatgcgcgc gctccagagc gggcagcacg ccgtggcgga tgcggttgcg
     3601 ggcgtatgcg tccgtatcat tggtggcgtc cgtcacatgc ggcacgccgc agcgcgccag
     3661 ataagccagg acatccgccc gctgcgccca cagcaacggg cgcacatacg gcccgcgcac
     3721 aggcgggatg cccgcggctc cgcgggccgc gctgccgcgg gccagattga acagcaccgt
     3781 ttcggcattg tcggagcagg tgtgcgccgt tgcgaccttc gcgccgtgcg cctgtgccaa
     3841 ccgtgcaaaa aaagcgtacc gttcctgccg gccccactcc tcgcccgcgt tttcaggcgg
     3901 cgagagcagc gtctcatcca gcggaacacc ccgcgccgca cactgggcgc gcacaaaggc
     3961 ggcgtccccg tctgcggcgg cgccgcgcag gccgtggttc acatgagccg cgcgcaggcg
     4021 cagcccccac acattctgca cggaaaacaa aaaatggaat aatgccatcg aatccgcccc
     4081 gccggagagc gccacgataa cagtatcccc tttttgcagc atttgatgtt gggaaatata
     4141 ggccccggcg cgggcttcaa actcatccat gggcatagtc cacgttggtg aatcgggtgt
     4201 gcgcgccgtc ccagcccagg tccaccttcc ccacctcgcc gtggcggttt ttggccacga
     4261 tgcattcggc tgtggtggcg tcggtttcct cgccgttgtc gtaataggcg tcgcggtaca
     4321 aaaacagcac cacgtccgcg tcctgctcga tggaaccgga atctcgaagg tcgctcagca
     4381 ccgggcggtg gtctgtgcgg ttcttctccg cgcttcggga aagctggctc agtgcaatga
     4441 ccggcacatt cagctctttt gccatgattt tcaggttacg ggtgatctca ctgatctcct
     4501 gcacgcggtt ctccgtgcgc ttgcccgtgc tcataagctg cagatagtcg atgatgagca
     4561 gcccgatgtc cgggcgcgcc gggtcctgat tgatgcggcg gatcttcgcc ttcatctccg
     4621 ggatggtgat attcgacgta tcgtccagat aaatatgggt cttggccagc acctcggacg
     4681 cgcgggccag gtcctcccag tccgccgtgg agagcccgcc ggtgcggaag gcctggctgt
     4741 cgatggccgc ctcgctggag aggatgcggc tgacgagctg gtcctttgtc atttcaaggc
     4801 tgaacacggc cacgggcaca ttctgctttt tcgccacgtt ggtcgcgatg ttcagagcaa
     4861 aagaggtttt gcccataccg ggacgggccg cgaggatgat caggtccgaa cggcccaagc
     4921 ccgtgagcat ggtgtccaga taattaaagc ctgtgggaat gcccagatat ttatctttat
     4981 tgggaccgga gatctcctgc agatgagcat aggtgtcgat gatgacgctg ctgatgggcg
     5041 tgagcgccgt ggaatcgcgt cccgagcgaa tttcataaat tttctgttcc gcgctctcca
     5101 gcagcaggtc ggcgtctgtt tcctctcctg tctgctccag aatggtcttg gccgcattca
     5161 tcagctgacg cacacggtat ttttccgcga taatcttcac ataggtgtcc gtatgcgaaa
     5221 gcgagggcac cgtttcagcc agctgggtca ggtagacctt ggccgcgtcc tcggtctcaa
     5281 aaacagcggc atcctgcacc gctgccagaa gcgtgacaaa atccacaggc gcgctgcctg
     5341 taaacaggcg cacgatctcg gagaatatct cgccgttctg gcgggcataa aacatctccg
     5401 ggcgcagcgt ctccaccatt ttgggaatga tgtctgcgtc caggatcgcc gcgcccagca
     5461 cagcctgttc ggcctccatg ctgtacggca ggtttacgcc gagtgattcc agcgacaggt
     5521 tgagcccgcc ggaaacagcg ctgcggttat tcgcgggcat tgcgggccct cctttccgta
     5581 ttccgaaaat ctcctgcggc cgcgcggccg cttattcctc caccttgacg gtcagcttcg
     5641 ccgtcacctt gggatatacc tttacctcga cctcatagga accgaagttt ttgatgtcgc
     5701 cgtccaatac gatcttgcgc ttatccaccg gctcgcccac tttctgtgtg agcgcggcgg
     5761 cgatgtcctt ggaggtgacg gagccgaaca ggcgcccgcc ctggcccgcc ttggccttga
     5821 tggcgacgat ttgcccattc agctttttgg ccagcgcctg cgcgttggcc agctcctccg
     5881 ctgcatgatg ctcgctggcc gccgcttttg tccttacctc gttgacggcc gccgcgtcgg
     5941 cggccacggc aaggccgcgg ggcagcagat aattgcgggc gtagccgtcc gatacctcgt
     6001 gcagctcgtc tttcttgccg atggacttta cgtcctgttt gagcactacc ttcatggtgc
     6061 agacctccct gtgtgtattg caggccccgc gccccctctg ctcacggcgg agaaaacgcg
     6121 ggccggatat ttttgcaacc gaaacaacta taccaatttt gtgcgggaaa ggcaagcggt
     6181 ttgcccggaa aatacatccg gcgcacggag aaaccgcgcg ccgggcaaac cgttttgca
//
LOCUS       DAWBSB010000134         6238 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_2374_length_6238_cov_3.742682, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000134 DAWBSB010000000
VERSION     DAWBSB010000134.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 6238)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6238)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6238
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2374_length_6238_cov_3.742682"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1104..1113
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1333 a         1903 c         1719 g         1273 t
ORIGIN      
        1 aaaacaaatc gcagtctttc ccgaacaccc tgtgtccgga ggcgccgctt ttcccctgcg
       61 tctggcgcag gccgccattt tgtcccctgt cgtattacag cacaagggac ggtgcggtat
      121 agacgcgggc ggccaactcg ctgttgagca tgtacagccc cttcgcgtcg ctcccaaaca
      181 gctccatctt ggtgatcaag tcagcggcgt ttttctcttc ctcgccctgc tcctttacaa
      241 accagtccag aaactgtacg gtgcggtaat cctgcgccgc ttgtgccgct gtgtaaatgg
      301 cgttgatgag tgcggtaaca taccgttcat gctccagcgc tgcggccaaa ggggcctcca
      361 gcgaagcaaa tgccccgtcc ggcttgccga cggcctccag tatcaccggc tggccgttgt
      421 tgtgcagata ctggtaaagc cgtccagacc cttagacgca taaaaattgg cgatgtccag
      481 ataaagatag gccgaataaa actccttgtt gatctgctgg ttgagcagtg cagaaacagt
      541 tgcttccatg acaataccct ccgttcagcg gcggctccgc cgctccataa ttagttttcg
      601 ctaaccacag tatagcacgg cagcggacgg tgtcaatctc ttttcacctt ttcgtccggc
      661 cttgctccca ttcttttgaa agcacagaaa gcgtgccgca tcccaaaccg gatgcggcac
      721 gctttttccg tttcaggcct ttcagcgagg cggggctccg tctgcgctga tgccccgcag
      781 aaatgcatca aataaaaacc gcgccatggc cggggcctcc ataccgtatt tcagcgacag
      841 aggcgcgcgg tcttttccca ggccgatgag tacatacacc gctgtatacg tggcgctgac
      901 ggcggtgagt tgcgcgtcca acccggcccg cagggagcca tcgttaaaat tctcgcataa
      961 aaatcgtgca gtgtcgccgg agccgaaatc tcccggccgg aacatccgct cgtaaacagc
     1021 gctgccacgc cgcgccgttt ccgcctgata ggttttttca aacaggtcga aaaataaaag
     1081 gaaattcggg tccagttcaa accnnnnnnn nnncaagctc ctctaaatac acggaaaagc
     1141 gttccagggc cgtgagcgga cgcgcctccg cccgcgccag cagtgcgttt ccaaatgtac
     1201 gcagcgcttg gcggtggatg gcccacagca aagattcctt atcggggaaa tatttgtaaa
     1261 gcgtcgcgcg cgcgataccg cagctgtccg ccactgcgct gaagcttacg cgctcgatcc
     1321 ctttttccac aaacaactgc cgcgccgcac gcaatatctg ctcatactgc cgcgcattgt
     1381 gcttttcata tttcatctgt ggcacggtgt gccgcctccc tctcttccgt tcttctccca
     1441 gtataccgca gacaccccgg gcagaaaaat catgtatgta aaccacacgc ccgttctttg
     1501 taaagttccg ataaagcctt tgaaaactgt ccatttttga ccggttttcc cacgggattg
     1561 cgttcttttt acatggctct tacatgttct gcggagaaag catcggggtc tatcttgtag
     1621 aatatgcaca gtgaaaagaa acaagattca acaaaatgac ggaacaaccc gtctaaaagg
     1681 aaaggatgga aataagtgcc acaaacgaat gaacggcttt tgtaccgggc gtccgggcag
     1741 ctgccacggg gctttcaggg gcacatctct tacacaacgc ttctgccggg ggagctgcgc
     1801 gccctgcgcg tggtgttcac ctttgacaag cgcgaaccgc agcaggagcc ggacacgctg
     1861 cgcgcgggct gtatggccgc tgccgcacag aacctgcccg aaggtacgcc cgtgccggag
     1921 gcgttggcgg aacatctttg cacgatgccc aagggcgaaa tcaacgtctc gctgtttttg
     1981 aacggtgcgt gtgtgggcag cgctcaccgc aaccaattga caaaggaaat gctgctcgca
     2041 ccgcagggct catccgaggg ctttgtaaac tgtcgtccca caggcgtact gcgggtggtg
     2101 ctgcacgtgc tcaacgttct caacgacgag acaaactaca cgctgcgcct ggaggaggtg
     2161 gccggatgaa attcataaga gcagaactgc acaaccattc cacagaatcg gacggtgcgc
     2221 tttctgtgga atcactggct gcatacgccg cggaaaaaaa cttcggcgtg ctggccctga
     2281 cagaccataa caccacttcc ggccatgcaa aggcctatga cgccatccgg cgggcgggat
     2341 acgcgcttgc gcttttgccg ggcgtggaga taaccacatt tttcggccac attctggcgc
     2401 ttgggcttgt acagatgccg gacatcacca cactggaccc ccgtgcgccg gagccttttt
     2461 tcgcaatgct gcgcactgcg ggcgcgcgtg ccgtgggcat cgcacacccg ttttccgtcg
     2521 gcagcccgct gttcatcggc tgccgttttg acatggaaat gcacgactgg aacacagtgg
     2581 actatatcga ggtctttaac acctctgttt cggaatccgg catgggtgcg caccccatgg
     2641 cagaggcgtt catcggcaac agccgggcgc tggcgctgtg ggaacggctg gtgctgaagg
     2701 gacagcgcat cgcggccgtc accggaaaag accttcacag catgccccgg gatgcggagg
     2761 tattcaccac ctacgccatc gtggacgagg cctgcacgct gaacgcggca gacgcggtac
     2821 tgggcgcggt gctgcgccgg cagaccatcg tcacaaaagg gcctctgttc accgcgcaca
     2881 gcgaaaaggg acgcgtaaca gtaatattcg acaacacctc cggctatctg gactgggcac
     2941 cggcgcaggc cgctgccccg gtgctggagc tgagggacag caccggagca gtacagcgtg
     3001 ccgaaacaga cctgcgcacg ccgctctctc tgtccctggc tccgggcgcg cgcagcgccg
     3061 tgctgaagct gtatgccggc gcctgcgcgc cggtacacct gttggccgtg ggggcgccgc
     3121 tttatcttga caaggaggga aacggatgag acgcctttat ttttcgctgg gcaaaaacaa
     3181 attctgggaa tatgtcctgc ttgcgctgtt tctgctgttt ttttacggac cgctgatgaa
     3241 tatgctgctg ctttcgttcg ccggagatta cgagtatccc gatgtcatcc cccggagcta
     3301 cggattcaaa tggtgggatt atgtgctctc caaggcacag cttgtacaat ccatcggcac
     3361 atcgctggtg ctggctgttg ttgtaacgct gctctcgctg gccgtatgcc tgcccgccgc
     3421 ctacgcgctg gcacgctacc cgttcagggg ccgcagcgcc gtgcgcctga gctttcttct
     3481 taccaacgca ttccccaaaa tgggcatcta cacagccatg gccgtcatct tttataagct
     3541 gaaccttatc ggcacatttg caggagtggt gctggtgcac atcgtaaaca cgatgatgtt
     3601 catggtgtgg attccttccg gcgcgttccg caccgtacat atccagcagg aggaaagcgc
     3661 ccgtgacgtg ggcgcttcgc cgctgcacac gttccttatg gttacgctgc ccatggccaa
     3721 gcccggcatc atcgtggctt cactgtatac gttcctcggt tccatggaag aggcgcaggg
     3781 ttcgctgctc atcggcctgc ccaacgtgca caccatgccc agtgagctgt atggcatcat
     3841 catgtccttc ccggataccg cgggcgcggt attcgcgatg atcctgctgg tgccgtccat
     3901 cctgctgctc gtcgtcttcc gcaaatatct gagcgccgac gccctgtcca acgggctgaa
     3961 aatgtgacgc gcccttcgca aaggagaaaa catggaaatg aaccttcccg ttatcaagct
     4021 ggagacccgc aatctcacca aaaagttcag cgctaccgag ggtgtagccg acatcagcct
     4081 ccaggtatat gaacacgagc tgttaacgct gctcggacca tccggctgcg gcaaaaccac
     4141 catcctgcgc gccatcggcg gctttaacaa gatcgattcc ggcaagattt tgctggacgg
     4201 cgccgaagta cagggcctgc cgcccgaaaa gcggcccacc ggtatggtat tccagagcta
     4261 caacctgtgg ccgcatatga ccatctacga gaatatggca ttcggcctga agcttcgcaa
     4321 aacgcccaaa aaagagatgg acgcgcagat cgcggagatg ctggcacttg taaaaatgag
     4381 cggcagcgaa aagaaatacc ccactcagct ttccggcggc cagcagcagc gtgtggccat
     4441 tgcacgcgca ctggtgctca ggcccgccgt acttctgctg gacgagccgt tttcggcact
     4501 ggacgccaag atccgcggcc agatgcgcga ggagctcaag cgcatccagc gcgaggccgg
     4561 gctgactgtc atcttcgtca cgcacgacca agaggaggcc atggccatct ctgaccgcat
     4621 tgccgtcatg aaccagggtg ccattgaaca gctggacagc cctaccgaaa tttacgacaa
     4681 cccgcacagc cgttttgtgg cggggttcat cggcgagatg aactttttgg acgaaggcgg
     4741 cgcggtgctc gccgtgcgcc cggaggatat tgctctgcgt tcggcctccg gcgcgggcat
     4801 ggcgcgtatt gaaagcgtga tgccgctggg ccattacaca cagatcatcc ttgtgcgcgc
     4861 agacggcgca cggctgaagg ttttcattcc caaggaacag gcactgggat atatacccgg
     4921 ccagacagcg gactattcct tcacgcgctt caagcagttc ggcgcggcac aggccagcgc
     4981 ctgaagtact tccgggcaaa acagcttcgg ctgttttccg ccggagcgga aagccgcccc
     5041 ggcaataaat aaggatttta tcgtaagctc ataggagata tggaagggag aaacattcat
     5101 gaatcgattc atccagaagg cgctgtgcgc cgtcgccgcc tgcgctcttg cgctgagcct
     5161 ttccgcctgc ggcagcactt ccccggccac gggcgccgca agcgcggacg ttcctaccgt
     5221 caccatctgg ggttatggag gccaggaggt gcgcgaagcg ctgcaggccg ttgccgacgc
     5281 acacaacgca gaccccgcct ataataccaa ggcccgggtg gagatccagt tcgtcgtctc
     5341 cggcaccagc gagcagagcc tgcccgaccg ccttgccgct gcctatcagg caggcgtgac
     5401 cgacaccgat tttgacatca ttgccctgga cgacagcggg atctccgcca ttctggccca
     5461 gacgagcgaa gattttttca tccccatcga cacctcgaag atccccaact atgaaaatgt
     5521 cgtattcaag gacaatatcg taggcgatac cttcatcccc taccgcggca cagccgtgat
     5581 gctggcctac aattccgaaa ccgtggaaaa tccgcctgca acggcggacg agctgtatca
     5641 atggatcaag gacaatcccg gccatttcgc ctactgcgac ccgtccaccg gcggctccgg
     5701 ctataccttt gtaacgaccg ccatctacaa ccagctgccc gacgaagcac gcatgtcccc
     5761 ggacaccaaa tgggagaccg aacacaccgc cgagtgggac aacgccttcg ccctgatggc
     5821 cgagctgcac ccttatctgt accagacggc gggcaaggta cagtacccga tgaagaatgc
     5881 cggttcgctg gacctgctgg ccacaaagca gatcgacatg acgcccgcgt tcgtgaacat
     5941 ggtgctcagc cagaagaata tgggtacgat gcccgagtcc atcaagcttc agcccatcga
     6001 gcccgccttt tccggtgcgc tggccggctt ctgcatcccg aaaatcgcca aggatcagga
     6061 ggccgcgctc tccgtcatcg actactacct gagctatgag gcccaggcca tcggctggaa
     6121 caccatgtat gcttctcctg tcgtggacac tgccaaattg gaaaatctgg accacgccga
     6181 ctggctggag gagaccaata tggacgagct gcgctatttt tccatcggca tgatacag
//
LOCUS       DAWBSB010000135         6222 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_2378_length_6222_cov_3.044430, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000135 DAWBSB010000000
VERSION     DAWBSB010000135.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 6222)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6222)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6222
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2378_length_6222_cov_3.044430"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1362 a         1848 c         1866 g         1146 t
ORIGIN      
        1 ccgcagcggt tcaggagaag ccgcacgctc ttagtacggt ttttccacca gccgcgcctt
       61 ttccagcaaa aggacgacgg caggcagcag cacgagctgg atgacgatgc ccggcagcgc
      121 cgttacgaac gcgcccgaaa caaataccgc gaaggaataa tttccggcgg agaagatgag
      181 cgcgttcatc acgccgtata caaggcggcc gatgagcatg gcgctcacga gctggatgta
      241 caggtccagc gggcgcttac ccgtgcgcac gaaccggcgc agcacgcccg ccgcaaggcc
      301 gtaggcggcc agctcgcaca gcatgccggg cagataagcc atcggcggca tgccggtgat
      361 catgctggag agcaggggag tgagaatacc ggacagcaaa ccgtagaccg gaccgcagac
      421 gagcccgcac agcagcacgg ggatatgcat gggcagcagc acgctgccgg cattggggat
      481 gctgtggaac gccacgggca gaacaacgcc cagcgccacg cacagcgcgg ccagcaccag
      541 ttgttttgtg ttgaacttcg attttgtcat aaacgatccc tttcctcaca gaaaaaagcc
      601 gcgaaaacaa ggccctgctt catgcggggc gcgtgccttc gtggctttct ggtttttatg
      661 atgatggatg cacgcgtgtg catgcaaagc cgcacggcgg ccgggacgcc ggggagatgc
      721 gcgccttcat ggcggcggtt ccctgttatt atagcagacg atgcggacgc tggcaagtat
      781 tttgtgcgcc gggccagtca gacgaacggg gtggtgtcca gtgtcagatc tgcaagcgcc
      841 tgcaccgcgt gggccttaaa ataggtttcc tccagcggaa tccagcgcgc ttcaaatacg
      901 gcccaggaat ccgcgccgcc ccgggctgtc agccgggcgc gctgtaccgc ctgcgcgcag
      961 gtgacgaaca ccgaggccgt ataatgcccg cgcagggacg gatgcagaga atagctgccc
     1021 tccacaacgg caaaggacgc ggcgggaatt tccacagcgg agccgggcat gcctgcggag
     1081 cagtcgaaag ggcaaaaacg cacgggttgt ccggctgcaa acggctccag cgcctcgcgc
     1141 agaaaacgct catggtgcac attttcaccg ggctgggcca gacgttccgg cgtgcgcagt
     1201 tccggcggca gaaaaaagtc gtccatgtga aagaccggac acccgaacac ctgcgccata
     1261 tgcgcggcca acgtgctttt tccgctgccg cagcgcccgt cgatggccag cagcgcatgg
     1321 ccgccggggg aaacggattc caaaacgcgc tgggccagca gcagagcagg ccaggcgtct
     1381 gcataacacc ggcgcaccac gcggtagtgg ggcgcatatt gctcccggta cgctgcgctg
     1441 tggtgtacgg ccgggcagcc tgcggcgcgc agagtcccgg tctgccgccg cagggtctgt
     1501 gcgtcccgtg gcggcagcac ggcgtccgcc caatgcgcca ggccgtccag cgcggcggcg
     1561 aaacaggcgg tttccccctt gtgttcacga gccgtggcgc agaatacgcg ccccaaaagg
     1621 cgcagcacct cacggctcca cgcgccgttc aaatagatgc ggcacagccc gttgccgata
     1681 tcctcgacac agcccggcag agcggcgccc tttacatgct ccggcacggc gtctgcgccg
     1741 gggagcccgg cggcccgggc atattcctcc atcaggtgcc gtccggcggt ttccggcgcg
     1801 ggggccgtgt ggccgcaccc gaattcccgc tggtacagca gtttgtaata atcctgcggc
     1861 tgcatctccg ggcgctgcgc ccggtgcgtg tccagcagcc gttccaattc ctgtctcatt
     1921 cagagaacgc tcctttatcg gcataagtcc gtgcagcaga gatgcactcg ccgctgcttt
     1981 tccagtatag cacacgaaac caggagattg aatagaccgg acacgatttt accgcccgtt
     2041 tcagccgttt ccacaagtgc gggcggcgcg ctgcctgcgg tgcccggcaa ggacgtgcgg
     2101 gctgaaacag agcgatggaa ataaaaaacg cgccccatac cttccggtac gggacacgtt
     2161 ttttggatga cgcggtgtgc ggaaggaccc gcgcgccgca aaggggagct ttttcaggcc
     2221 ttccacaggc tgtgcaggtt gcagtactcg tacgcggcca caacgctgtc gccctcggcc
     2281 agcataaaca cggcttccgg cttctggccc ggcgccaaag gcttacgctg gttgccctga
     2341 gccgtctcca gcgcgatcca ttcgatgtaa tgctcgggca gcatggggtg ttccacagcg
     2401 ccgacgcgca cagtcacctt gttgccctct acctcgatga caggcacgtg cttttccaat
     2461 gccccgtccg tagtgccggg gatgatctcc tgcatcggtt cgccgcagca cacaacgggc
     2521 acgccgctgc tgcgcacata agcgatgata tttccacaat gtttacagat ataaaacttc
     2581 atatccgatc ctccatcctg atgcaatatt ttgccgcgcc gggcggcgcg gcagccggaa
     2641 cgcaaacgcc ccgctgctta cagcatgaca caaccggacg cagtttgcaa gagctttttt
     2701 ggaaaattca ggtaatccat caactttgta ggaatgcaca gttgtacttg tgaaaaagga
     2761 cggagcgtac ggcgcgtgag aggcggcaga tcacagatcc tttatcaata ccagttcaga
     2821 gacgcctttt cggacggcgt cgggcagacg gcccaattcc caatagccga gggagaggta
     2881 cagggcctgc gccttttcgt tgaagtcgct caccatcagc gcaacgcgtc cggctccttg
     2941 cacacgggcc atggcttcga actccgcgag cagaaaatgc cccacgccgc gcccgcgggc
     3001 ccggggcgcg gcgccgatga gcgcgaggta gggatacagc ccgcaaaagc ccttggcccg
     3061 gacccgcatt acgcccagca cctctcccgc actaccgcag gcaacgtaaa gttcgcctgc
     3121 gttcaatgcg ttttcgaggg agcgcgccaa tgccccgggc tcggaaaagt aacgttcata
     3181 aatttcgctg tctgcaaata cattgcagca ggcgggaaga tcggcctgtg cggcggcgcg
     3241 gcagatcaat tccatggcgg cgcctcctgc tgcatgcgtt cggccgcggc ggccgcggca
     3301 ttgtggatca gcgaacgcat gcggatgagc tttccgtttt cccgggtggg gcatacgcgg
     3361 ttggtcagca gcaccacaaa aagcccggta tcggggtcca cggcgatgct ggtgcctgtg
     3421 aagccggtat gtccgtaggc gcgcgggctc atcagatcgc ccaaaaaatt ggaggggctg
     3481 cctgccaggt tgaagcccag gccgcggaat tcgtgcccgg cacccggtgt gcggttgacc
     3541 agcgcggcgc gcagcgttgc aggggaaaga aactgtgtgc cgtccggcag tttcccgccc
     3601 agcgccagca tctgaacaaa gcgcgtcatg tcgcaaagat cgctgaatat acccgcgttg
     3661 cccgaaagac cgcccagaaa ccgggcgttt tcatcatgga cggcaccgcg cagcagctcg
     3721 cccgtggccg ggtcccgttc cgtgggagcg acatcccccg ccgggcggta ggtagtgtgc
     3781 gtcaggccca gaggctcgaa tacatactcc tgtgccagcc ggtcgatgga ctggccgccg
     3841 atttgttcca aaatacgccc cagaaggatg tatcccatgc agctgtaaac gggcaaagcg
     3901 cctggcgcat gctccagcgg gtgggcaagg atcgcccgga cggcgtctcc ggggccgtcc
     3961 gcttcctcgc tgagataaaa atgggcgggc aggccgctgg tatgtgtcat caactggagc
     4021 acagtaatgc cttttttgtc ttcgggcgcg gggaaaaact gttccactgt atcgtaaagg
     4081 cgcagctgcc cttcctccag aaagcggaat gcgatcatcg tggtgcccac gatctttgac
     4141 aggctggcga tgtcgtacag cgtagtctcc cgggcggggc cgaaggtctg ccggacacag
     4201 acgctcccgc ggatacccac ggaaaggcag gcggagggat aagcgccgtc atccaggccc
     4261 tgccggacaa gacggcacac ttgctcaaaa tttttcatgg cagcctcctg aaaaaatagt
     4321 ttccgaaccg cgcggcggcg ggaagtcacc tagatggtag tgcacgaagc ggaaaatgtc
     4381 aagaaggcac agcggggcgt ttttaacaaa atcaaagaaa aaactatgga caatataact
     4441 gtttttttga atcataaaaa ataattctgt tcatattgtg cagttttttc cggtttttgt
     4501 attgacaaag caaaggaaaa agcattatga tatgagcatg aaacaattgt tctgaaaatt
     4561 ttaagattat taaaattaat tttctgaacc gtttcaaatc ctgccgaatg tattgcgcag
     4621 taataaagga ggacaacatg aaaaagtcgc tgaagaagat cctggcattt accatggctg
     4681 cggcaatggc cgccggtcta gctgcctgcg gcagctctgc atccacgtcc ccgtccgcgg
     4741 ggggctcggg cagcgccccg gccgtttcgg acgaacccat caaaatatcg atgtattacg
     4801 cagataaccc cacgctgccg tatatggaca gctggctggc ggttcaggag actgcgaaaa
     4861 tcgcgggtgt ggacctgacg gtggaggcta tcccttccac ggattacaat accaaggtct
     4921 ccctggcgct gaacaccggc gagaattgtc ccgacgtgat actttatcag gatactaagg
     4981 gcgagaattc ttcgctggcg ctcaacggag ccatcgtccc catcagcgat tatccggaat
     5041 ggacgcccaa ctttaacgcc atggtggaaa agctgggcct gaaggatgat gtggaggaac
     5101 tggcgctgaa ggacggcaag cgttatttta tgccgcagct gttcgaccag cccttctatg
     5161 acggcggcct gatcatgcgt caggattacc tggaggaaaa gtgctctgat gcgcccaaga
     5221 ccttcgatga tctgtacgag atcctcaaag cctataaggc ggaccatccc gattcctacc
     5281 cgcttaccac gcttgtcgcg ccttatgtta cataccgcat gacgatgcct tccttcggca
     5341 tcagcttcgg aaagagctca tcctccggca cgggcgcgct gagctacgat tatgaaaagg
     5401 gcgaatattt cgagggctgc atcagcgagg aagcccgcga atatctgcgc ttcatgaata
     5461 agctgtatgc ggaaggcctg ctcgaccccg agatggctgc ccccatcgac ggcgacgtat
     5521 ggtccaccaa gatggcaacg ggcgccgcaa tggctactta cgcttactac gaccagatcg
     5581 gcggcgttga gaccgccagc gagatcgacg gcttcaagct ccagatgtat ccgtccctgg
     5641 aaggcccggc cggcgcacac catcagccca aatccaaaac cggcaagggc atcatgttcc
     5701 cggcttccac cgccgagcgc gacgatttcg agcaggtagt acgcgccgtg gatgagatgt
     5761 tcttctcgga ggaatgctcc acgatctggt gcctgggcgt ggagggtacg acctacacca
     5821 tggacggcga caagatcgtt tattccgacg acctcacaag ttcgcccgac ggcatttaca
     5881 aaaccatgca ggtgaagtac ggctgcggcg cggatccttt ccagtttgtg tggatcaacg
     5941 aacgcgaaat gagcaagtat gacgagaact acgcccagat caacaaaacc gtcgcaggca
     6001 tggaggatgg caatgtgatc cagccgctgc ccccgacgcc gctgtttgac gacctgacgg
     6061 ccgaagatgc ggccagcctg cagacgccgc tggcggacag catcgaggta tggatcgacg
     6121 cattcgtcac cggttcaaag agcctggata ccgattggga cgcctatgtg gcagagatga
     6181 aaaacctgca gatcgaagag ttctgcaaga tgtacaacga ca
//
LOCUS       DAWBSB010000136         6030 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_2456_length_6030_cov_2.876820, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000136 DAWBSB010000000
VERSION     DAWBSB010000136.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 6030)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6030)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6030
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2456_length_6030_cov_2.876820"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3873..3882
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5479..5488
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1148 a         1909 c         1703 g         1250 t
ORIGIN      
        1 ctccggcgcg ttatccacag cgggcggcgc ttcctccgcg cccgcgtctt ccagttcgtc
       61 cccgagcagc gaatccccct ctgtggatac ctccggctgg acgcgccgct tcatatccct
      121 gcctgtcatg catccggacg gcggcgccgg tgaaaaagcc ctcgtcatcc tcagcgcgcc
      181 cacggactgg gagtacgaca attacacaac ctttacgcgg gacggtatca aggttgccga
      241 agttctgaac ctgtggcccg ccgcctccgg tgcgtctccg ttcacagacg ccatgacgga
      301 gccgtatgcg gacaacagct tttaccccga gggatacggc ctgctttcca ccgaagacac
      361 ggagctgaac ggcaatgcgc tgcgcatcct gcatatgaag acctggcccg atgacgcgga
      421 caagccctgg tatccccagt atatgtttta tgaactggac ggctatgtgg tggagctgca
      481 tttccttacg gatgaagaga acggcggcag cgcggacatt tttcgcgccg tactgggcac
      541 gatggaattg catttcagcg aaaaagaggc cggcggcagt tcttcttcag gcggcgcctg
      601 accggctttc ttcttcatcc tcttttctca tcctttgcgg ggcggcttcg gccgccccgc
      661 ttttatcagc tttgcaaagc cagaccagcg gagtgttccg gcccccggcc ctaaaattac
      721 ggtgtgggac gcaaagtgga cggatgcata taaaaatatg ccggatcggc cgcggccatt
      781 tttataaaaa tcctgttttt cctattgaca gcgtgtgtat acaggtatat attgtatata
      841 cagtatatat acaatatttc cgaggtgcct catggatatc atcatcagca atgccagtgg
      901 taagcccatt tacgagcaga tcacagtgca gatcaaaagc cagatccttt ccggcgcgct
      961 cacggcgggg gacgccctgc ccagtatgcg cacgctggcc aaggaactgc gcatcagtgt
     1021 gatcaccacg aaacgcgcct acgaagacct ggagcgcgac ggctttattg aaaccgttgc
     1081 gggaaagggg agcttcgtgg cctcaaagaa cgcagaattc ctgcgcgaag aagcgctgcg
     1141 gcgggtggag gctgccttac agcaggctgt ggacaccgcg tcgcgcagcg gcgtatcgcc
     1201 gcaggagctg cgccagatgc tggaactttt attggaggga aattagaata tgaacagcgc
     1261 gatcgaagtg cgcgggcttt gcaagcatta cagggatttc tccctgcggg acgtctcgtt
     1321 taccgtaccg ggcggctcta tcatgggctt tatcggcgag aacggcgcgg gcaaaaccac
     1381 tacaatcaaa tccattctgg gcctgctgca cacggacgga ggctccatct cggtactggg
     1441 gcgcgaccct gtcacggacc gccgcgccat cgggcaggat atcggcgttg tgctggacgg
     1501 cagctttttc tacgagggca tgcgcgcgaa ggatatctcg gccgtgatga gccgcctgca
     1561 cagccgatgg gacaaagagt cgttcgcgtc gtactgcgag cggttcgctc tgcccatgga
     1621 aaaaccgttt aaggaattct caaagggcat gcgcgccaag ctggcgctca ccacggcgct
     1681 ggcgcaccat cccaaactgc tggtgctgga cgaggccacc agcgggctgg accccgtagt
     1741 gcgcagcgaa atgctggatc tgtttctgga attcattcag gatgaggagc acagcatcct
     1801 gctctccagc cacatcacca gcgacctgga aaaagtggcg gactatatca cgttcatcca
     1861 cgcgggaaag atcgtgttca gcaaaacaaa ggacgatatc ctggagcagt acggcgtggt
     1921 gaaatgcgaa aaagcgcgcg ccgcggccct ggacggcgcg catatattgg gccggcgcga
     1981 cagcaaattc gacgccgagc tgctggtgga caaccggccc gaagtggccc gcctgtaccc
     2041 cggcatactg acggagcagg ccaccattga cgatatcatg actttttttg ccaaaggagc
     2101 gtacgacgca tgaccggact gatgctgaaa gacatgtatt ctcttcgcac ttattttctc
     2161 aagcagctcg cgctgatgac tgtgctgtat ttggccatct ccgtgggcct gatgaaaaac
     2221 ttctcttttc tttccccgat gctgacaatg agcgtggtga tgatgctcat ttccagcttc
     2281 agcgtggacg aagcatccag ctgggattcc tacgcgctga cactgcccgt cacgccccgg
     2341 gccatcgtgg gcgcgaaata tctgcttttt tacggcgctc tgctgggtac gggcgttatc
     2401 gcatctgtcc tgtgcggcgt gctggacagt tttacctacc gcaacggctt tcttgagatt
     2461 gcggccagca ccggcggcgt catcgtcgta tacatgcttg tcaccgtgtt catgctgccg
     2521 ttgtttttta agctgggtgc agaaaaggcc cgcgttgcca tgacactgtg ctttatcctc
     2581 ccctttctgc tcatcgtctg gggcttcagc tggctcggtg acggagccgc agcttttgtt
     2641 gagagccttc cctggccgat gatcgccacg gtgggcggcc ttattctggt gctgctgtgc
     2701 gccgcctcct ttttcgtgtc tgtgaaattt tacgagagca aggagtattg agatgaaaaa
     2761 cagccgtcgc tgctccaaat gcggcggttc ggacatcgtc cgtataccgg accgccgcgg
     2821 ccgctgcgcc agcgggaaca atatttatac ttccaacttt gaaaacttcg gcaagatacc
     2881 ggtcttccgc tatgtgtgct gcagctgcgg ctatgtggaa aactgggtgg aagatcctgc
     2941 ctccctgcag gccatccgca cgcgcttcgg ttgacttgca aatggaaaac cgctcccgcc
     3001 gcacgcccat tgaatgggct gcagggagcg gttttgtttt tcttttatgg ttcagcggcg
     3061 gcgctctgcg cgggccgtgc ggctctatcc gcgggcctgc ccgtaatatg cgcccgggcc
     3121 gtgctttcgc aggaagtgct tgtcctgcac atattggggc gcaggagccg cgcccgggtc
     3181 gatctgaacc gtcagaagcg ccattttcgc cacttcctcc agcaccgcgg catgatatac
     3241 agcctgagcc gcgtctttgc cccatgcgaa aggcccatgg ctgcggcaga tgacaccggg
     3301 cacatggacc ggatcaatgt ccctcgcacg gaatgtctca acgatgaccg ttccggtatt
     3361 tttctcgtaa tcttcttcca cctccgcgcc cgtgagagag cgcgcgcagg gcaccggccc
     3421 gtagaaatag tccgcatgtg ttgtgccgta gcacgggata tcccgtccgg cctgtgccca
     3481 ggccaccgca tggacgctgt gagtatgcac cacgcctccg atctcaggga atgcccgata
     3541 cagctccaga tgcgtttttg cgtccgatga cggatggagc gccccttcca cgctgtttcc
     3601 attcatatcc agcaccacga ggtcttccgg gcgcagcgca gaatattcca cccccgacgg
     3661 cttgatgacc atcaggccct tgctccggtc aataccagac acattccccc atgtgtatgt
     3721 gacaaggccc cggcgaggca gctccagatt ggcctcatat accactttgc gcagttcttc
     3781 cagcatatgg cctcccctcc cttagcgcag cgcggccgcg gcggcttttt ccaccgccag
     3841 cccgcttttg taatccgtca gaaagcgcgc gannnnnnnn nncccgccgt atcggcaccg
     3901 tccggctgct gcacgctgcc ctgcgccccc cggaacaccg tgtcagccag atattcttcc
     3961 agtgtttggc cttcggcccg gcgcacgcgg taagccgcca gaagcgccat cccccaaggg
     4021 ccgccctctc ccgctgtttc catcacggcc acaggcacgc ccagcgcgcc cgccagcagc
     4081 ttctgcccca ccaccggagt cttgaacagc ccgccgtggc ccagcagcgt gtccagccgc
     4141 acgccttcct ccaccaacag gatatccatg cccagcttca gcgttgccat tgccgcgtaa
     4201 agctgcacac gggcaaaatt ggccagtgtg aagcgtgcat ccggacggcg cataaacagc
     4261 ggccgtccgc tctccacacc tgtcaccggt tcgccggagt aatagttgta gctcacaagg
     4321 ccgccgcaat ccgcttcgcc ttccagcgcc ttttgataga acaggtcgta aaggcgcggc
     4381 ttttccagct tcgcgccggc ggctccggcc atttcgccca gcacgcgcac ccatgcgtcc
     4441 aaatcagatg tgcaggtgtt gcagtgtacc atcgccaccg gtcgccccgt gggggtggtg
     4501 accatatcga tctccggata caccctggcg agcgggcgtt ccagcaccgc catggcaaac
     4561 acgctggtgc ccgccgaaac attgcccgtg cgcacgccca cgctgttggt ggctgccatg
     4621 cccgtgcccg cgtccccctc cggagggcac agcggcacac cggggcgcag cacgccggtg
     4681 ggatccagca ggcacgctcc ctccgccgtc agcgtgcccg cggccgcgcc ggcgcaaagc
     4741 acccggggca gaatatctgc aaggcggtaa ggcatacctt cctgcgccag aagcgcgtca
     4801 aatgtttttt ccattcccgc gtcgtaaccg catgccacgc tgtcaatggg gaacatgccc
     4861 gacgcttcgc ccacgcccag caccctttcc cccgtcagcc gccagtgcac atagcccgcc
     4921 agcgtggtca agtatgcaat gtcccgcaca tggggctcgc cgtccagtat cgcctggtac
     4981 agatgcgcaa cgctccaccg ttgtggaacg ttgaacgcaa accgctccgt cagcagcgcc
     5041 gcggcgcgtt ctgttgtcgt gttgcgccag gtgcggaagg ctgcaagctg cgcgccgtcc
     5101 cgcgcaaagg gcaggtatcc gtgcatcata ccggaaacac ccattgcgcc cacggtatgc
     5161 agcggcgtgc cgtaccgctc ctgaacctgc cgggccagcc gggcaaatgc atcctgcacg
     5221 cccgcccata cgtcatccag ggcataggtc cagtatccgt gctccagacg gttttcccaa
     5281 tcgtgcgcac cgctggccag cacagcatgc tccggcccaa tgagcaccgc tttgatgcgc
     5341 gtagagccaa gctccagccc cagcgctgtt ttttcaagct gtatttccgc catgaatctc
     5401 cttcctctca tctccgacaa ttccaatcga acggccgggg tgcgcgatgg cgcatcccgg
     5461 ccggagtttt ccttttccnn nnnnnnnnaa caaacccgta aatattatac aaacggattc
     5521 tcgccggggt acggcaggct cctgggctga ttgtaggcaa tgtccgtcac gcccagatac
     5581 ttgaacacct caaacagcgc cttgccccag tgcccgaacg ccaccgcgcc gtggtgcggg
     5641 taacgctttg caatcagtac atggcggtag aagcggtcca tctccggaat ggcaaacgct
     5701 ccgatggagc cgaagctctc gcagtccacg tccagcacct gcccctgcgc gacgtatgcc
     5761 ttcagctgtg tgtccgcggt ggactgcaga cggaagaacg tgatatcgcc gggcttgatg
     5821 ttgccttcca tggtgccgcg tgtgatgtcc ggctccggca gctccggctc cagatcgcgt
     5881 ttcatgatga gctgatagcc catgtgcggg tctttcagca tcgcacagct ggtgttgccg
     5941 caatggaagc ccatgaaggt ctcgggcagg cgcacatcgt atctgccccc gatggctttg
     6001 tcgtaaatgg atgcgggcac ggtgttgttg
//
LOCUS       DAWBSB010000137         6012 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_2464_length_6012_cov_3.497566, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000137 DAWBSB010000000
VERSION     DAWBSB010000137.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 6012)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6012)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6012
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2464_length_6012_cov_3.497566"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2895..2904
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5223..5232
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1003 a         1719 c         1986 g         1284 t
ORIGIN      
        1 ttatcgcttg ctgaactgcg gagcgcgacg agctgctttg agaccgtatt tcttgcgctc
       61 cttcatgcgc ggatcgcgcg tcaggaagcc ggctttcttc agttcgccgc gcaggttctc
      121 gtcataaacg agcagcgcgc gggacaggcc gtggcggatg gcgcccgcct ggccggaaac
      181 accgccgccc gcaacgcgca caacgatgtc gaatttgccc tcgacgcccg cgacagccag
      241 cggctggcgc acgatgagct tcagggtctc aagaccgaaa taatcgtcga tatcacggtc
      301 gttgatggtg atcttgccgg tgccgttgta cacacgaacg cgggcaacgg aattcttacg
      361 acggccggtg ccgtagaaat aaggtttcgt ctcgtacatt ctcattgcct cctattacag
      421 tgtgtaagct tcgggcttct gggcggcgtt gttgtgctcg gcgcccttgt agacgcgcag
      481 gcgcttcagc gcggcggcac ccagggtgtt gccgggcagc atgcccttca cggccagcat
      541 catggcctta tcggatttct cggccatcag cgtgcggtac tgcacggctt tcatgccgcc
      601 gatccagccg ctgtggtggt agtaatactt cttgtccagc ttgctgccgg taaggacagc
      661 ctggtcgcag ttgatgatga tcacatggtc gccgcaatcg acattgggcg tgaaggtgac
      721 cttatttttg ccgcggagca gcacggcggc agtggcggcg gtgcggccca ggggcttgcc
      781 ggcggcatcg atgatgtacc acttgcgttc cacggtgccg ggtttagcaa gagttgtgct
      841 catggtgttt tcctcctaat agtctctaca aacaatcgaa aggcccgtgc aaacaggcgc
      901 aagattgatt atagcggatg cccggcgccg cgtcaacccc ttttttgcat ttttttgatt
      961 tagaaagcga gcgctttaaa atgctctgtt ttgctcttga atgctcttga atgctcacgt
     1021 gctgttttta taaaaatttg cccgcttttt gtttactctg cccacgaaaa tgttatactg
     1081 gaagaagcgg ataaggcggc atgccataaa gataaaggaa ggccggtgga tacgatggaa
     1141 tttgaacggc gctgctgggc cgaggtggac ctcggcgcgc tgcggcacaa ctttaagctg
     1201 gtgcgggaaa gggccgggga tgcggcggtg atggccgtgg taaaggccga tgcatacggc
     1261 cacggcgacg cggtggtggc cccgcttctg gagcaggagg gcgcggacca gtttgcggtg
     1321 tccggctttg gcgaggcggt gtgcctgcgc cgggcgggcg ttacgaagcc catcctgatc
     1381 ctgggttata ccggcgtgca gaacgcggcc gcgctggccg tgggcggcat cagccagacg
     1441 gtgttctcgc tggaatatgc gcgggcgctt tcggaggccg cgctgcgtgc gggcgtggcg
     1501 gtgcgcgtgc atttgaaagt agacacgggc atgggccgca tcggctttac ggcggacgac
     1561 gatatggagc gtgccgtggc ggagatggcc gaggcctgtg cgctgccggg gcttgtgccc
     1621 gtgggtgtct ttacgcattt cgcagcggcg gacagcaccg cgccggagga tatcgcctat
     1681 acgcgccgcc agtacgatat tttgtgcgcg gcagtggagg agatggccgc ccggggctgt
     1741 acctttgcgg tgaagcattg ctgcaattcg gcgggtacct tcgcctggcc ggagttccat
     1801 ctggacatgg tgcggccggg catcatcctg tacggcgaac agccctcggc tgatacggtg
     1861 ctgcccgggc ttgtgcccgc gctgcgcctg cgcgcggcgg tatccatggt gaaggaactg
     1921 gcggcgggcg acgccgtcag ctacggctgc accttccgcg cgccaaagcc catgcgcgcg
     1981 gccacgctgg ccgtgggcta cgcggacggt tatccgcggg cgctgtcggg ccggggcacg
     2041 gtctcgctgc acggcaggcc tgcgcgggtg ttgggccggg tgtgtatgga ccagatcgtt
     2101 gtggacgtga cagacatccc ggacgtgaag gccggggatg cggctattgt gttcggcggc
     2161 ggcacggcgg acagcgtaga cgacgtggcc cgcatgacgg gcaccatcaa ttatgaagtg
     2221 ctgtgcgacg tggggcgccg cgtgcagcgc gtatatgtgg aaaacggcgc agaagtggaa
     2281 ttggtggact atctgaaagg ggaatagacg atgcgggagc tgcttgttgt gattgactac
     2341 cagaacgatt ttgtaacggg cgcgctgggc aacccggccg ccgccgcgct ggagccgggc
     2401 attgccgcgc gggtggccgc gcagttggaa aggggcggcc gcgtgctgtt tacccgggat
     2461 acccacggca cggattacct gaacacgcgg gagggccgct ttcttcccgt ggcgcactgc
     2521 attaagggca gccagggctg gggcctgtac ggctcattgt cgcagtatga gacgggcgtg
     2581 cgggacggcg tgtccattgt ggacaagccc acattcggct gcgcggcgct gccggccgag
     2641 gcagaggcgc tgtgcggcgg cgcgccggac agcattgaga tctgcggcgt ggtgacggac
     2701 atttgtgtgg tgagcaacgc cgttttgctg cattcggcct ttctgaatgc ccgcgtggcg
     2761 gtattgaagg acctgtgcgc cgccgccacg cccgaggggc acgcccgtgc gctggccctg
     2821 ctggcgggta tggggtatga gatcgtgtga caaagcgccc ggggcgcgat gaagaaaaca
     2881 ggaaagcgcg gacgnnnnnn nnnncttttg ttttgtgtaa aacggctcag gaatattttt
     2941 tcacaacggc ggccatctcg tcccagcggg cctccatgtc gcgcaccagc ttgaactggg
     3001 tgcggcagcg gtccaggttc tgtccgtccc ggtggatctt gaagtaggtg tcgccctcca
     3061 gatagtccgt caggaagcgg atgccgcact ccagcgtcat cagcttcgcg ccccagggca
     3121 ggtactcctt ctctgcgtcc gtcagcgtgc cggccgcgcc ctcgagaaag cccttggtgt
     3181 aaatatcaaa cagcggaagg tcgaaattca ccttggaaag atcgcgctcg tcctcggcgg
     3241 aatggttcgc gccgaagcgg atggaatcgc cgaaatcatt gatggacagg cccggcatca
     3301 cggtgtccag gtcaatgacg cagatgccct cgccggtgtc cttgtcgatg aggatgttgt
     3361 tgagtttggt gtcgttgtgc gtcacgcgca ggggcagcct gccggcgcgc agcgcctcca
     3421 gcgccacgct gcaatccgcc gcatgggcct ttacgaacgc gatctccggc gcgcagtcct
     3481 tcgcgcggcc cagcttgtcc gcagccagcg ccttttcaaa attggcgagg cggttttcag
     3541 tgtcgtggaa tttttcaatg gtctcgaaca gggtatcggc gggataatcc ttcagcatgc
     3601 gttggaaatt gccgaacgcc ttggcggaag catagaacag ctccggcgtt tctgctttct
     3661 gcaggcacag cgtattttcc acaaagggat acacgcgcca tgcgccgccc tcgctgtcgg
     3721 tgaaatatgc cgcgccgttt ttggtgcgca gcaccgtcat ggtttcccgc gccgcgtcgc
     3781 cgccgttttt ctcaatgaga tcgcgcagat agtccgtcac gcccacgatg tttttcatga
     3841 gctggtccgg gtgcttgaat gcggcggcgc tcatgcgctg gaggatgtag cgcacgcagt
     3901 cgccttctgc cgtctgggtg tatacgcaga acgtgtcgtt gatatggccc tggccgaagc
     3961 gcgccgcgcc tacgacctcg cccgcgaacg cataggcggc aagcacttcg gagagcgtgt
     4021 tttcagcggg atttgccatg cttacacctc ccacagattg tcggggtaca ggccctgcgc
     4081 cgttttttcg gcaatggccg ccaccaccac gggcttatct tctttgtagg tcacgccgta
     4141 ccacttgtcg cggctgtgca gcaccttcac ctgcgccttc tcctcggcga tgagctgact
     4201 caccacgctg gggaggaagt actcaccctt aagggggttc gttttcagcg cgttgtccag
     4261 aaacgccgca aagcgggccc cggtctcggc cagaaagctc cgggtaaagc cccacatgtt
     4321 catggatacg atggtgtcgc cgggcaggtc cgtccaagtg gcgccgtcat cctctgtgaa
     4381 gcgcgcgccc gctgcggttt tttcgatgtg ggtgcgttcg gtgacgctgt ccagatagcc
     4441 ctccgcattc gtcacgcaga tgccgcgggc cacgtggccg ttttcggtta ccgtgttctc
     4501 cagcagatag ccgaccatgg cgtattcata tttgccgtcc cggtccgggt tggcgagcag
     4561 gtaatcgtag atcttctgga acgcctcggg gccgtaatag tcgtcggcgt tgatcacggc
     4621 gaacgggccg tcgatgacgt cccgggccga caggatggcg tggctggtgc cccagggctt
     4681 gacgcggcct tcgggcacgg cgtagccggc gggcaggtcg tccagctgct ggaacgcata
     4741 gcgcacgtcc atcactttgg acaggcggtc gccgatggcg gccttgaagt cggcctcgat
     4801 ctcgtgcttg atgatgaaca ccacggtctc gaagcccgcg cggcgggcgt cgaagatgga
     4861 atagtcgatg atgagctggc cgtggccgcc caccgggtcg atctgcttga ggccgccgta
     4921 gcggctgccc atgccggcgg ccataacaac gagtacaggt ttgttcattg cgttattctc
     4981 ctttttgctc cgattcttgt atccaagggc gggcgcggct cagcccttgt agccgttggg
     5041 gttcatgctc tgccatttcc agctggaggc acacatctct tcaatgccgt actcggccgt
     5101 ccagcccagc tcgtcccggg ccttggcggg gtccgcgtag cattcggcaa tgtcgccggg
     5161 gcggcgcggg tcgatgacat agggcaggtc cttgccgcat gccttgctgt atgctttgat
     5221 gannnnnnnn nngctgtagc ccttgcccgt gcccaggttg cagatgaaca ggccgcagtt
     5281 cgaagccagc tttttcagcg ccgccacatg gccgcgggcc aggtccacca catggatata
     5341 atcgcgcacg ccggtgccgt cgggcgtggg gtaatcgttg ccgaacacat gcaccttttc
     5401 cagcgtgccc acggccacct tggcgatgta gggcaccagg ttgttgggga tgccgttggg
     5461 gtcctcgccg atgaggccgc tttcatgagc gccgatgggg ttgaaatagc gcagcagggc
     5521 cacattcatc gtggggtccg ccttgcagta atcctttaaa atctgctcca tcatcacctt
     5581 ggtggtgccg taggggttgg tggtggcgcc ggtggggaag tcctcggtga tgggcacgct
     5641 ggccgggtct ccgtaaaccg tggcggagga cgagaacaca aagtttttta cattgtattt
     5701 gcgcatggcg ttcagcaccg tcagcgtgca gcccaggttg ttggtgtaat attccagcgg
     5761 tttttgaacg ctttcaccca cggccttgta tgccgcgaaa tggatgacgg cgtcgatctc
     5821 cggatgggag gcgaacagcg cgtccacggc ctcgccgtcg cacaggtcca cattgacgaa
     5881 ggggaccttt ttgccgctga tctgttccac gcggcgcagg gcctcctcgc aggaattgta
     5941 caggttgtcg gccacgatga tctcataacc cgctttcatc agctcaatgc aggtgtggct
     6001 gccgatgtgt cc
//
LOCUS       DAWBSB010000138         6000 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_2471_length_6000_cov_3.846426, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000138 DAWBSB010000000
VERSION     DAWBSB010000138.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 6000)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6000)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6000
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2471_length_6000_cov_3.846426"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2897..2906
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          995 a         1838 c         1812 g         1345 t
ORIGIN      
        1 acgtaggcct ccacctcgcg ctcaaagaag cccagcacct tgatggtggc gatttccttt
       61 gtgcgctcgg tgatattgat gttcatcagg ttgtacagta ccacaaaagc cagcatgccg
      121 gcacacagga tgatcacgta cacgacatag ttgatgctcg cgatggtgtc gtccagactc
      181 gagcgcatgc tgctcatctg cgtgaggccc gctaccccgt ccacctccag cagggatgtg
      241 gagaaggctt cgtccacatc ctgcgcatcg tcgggcagga tgcccagcac agagttgtat
      301 tccagcgcgc tgccgtaggc gtcctcataa acggagggcg tcatatacac ataattttcc
      361 acataatttt ccgctacgcc cgtgacggtg aatgtgccct cccggccttc gccgttttcc
      421 agcgtgagcg tgtccccggg cgaaaggccc gcttcgtcgc tgaacttttc cgtgatgacc
      481 acgccgtcgt ctgtcagcgg tacggctgcg ccgcttttgc gttcatgcag gtcgataaaa
      541 ttgtccagcc gtgcttcatc ctcgggtaca aagaggtata cgtcatagct ctggccgtcc
      601 acatccagcg aaatgcgctc ctgctgtacg ggcaggtagc tcgtgacctg ttcgggagca
      661 tccatcagcg cctggaatcc ggcatccttc aaggcgctct ctttgctcag tgtcacgagg
      721 aaatcatagg tgaacacctt gccgaactgt ttggatacga tgtcgctgat ggagtccttc
      781 aggccgaagc ccgtcaccag cagcgccgta cagccagcca cgccgatgac cgtcatgaaa
      841 aagcgctttt tgtatcggaa caggttgcgc gccgtcacct tgtgggtgaa cttcatgcgc
      901 ttccataccg gggtgatgcg ctccaggaag atgcgcttgc ctgccttggg cgctttgggc
      961 aaaaggagct gtgcggccca ctctttcagc gtctggccgc aggcgttcag tgtggcggcg
     1021 ctggtgcaca ggagcgccgc gcccacgccc agcacggcca gcgaagcgtg gaacagacag
     1081 cggaacccgg ggatggtata catggtgccg taggcgttcc agatgacgat gggaatgacc
     1141 gtaaagccga aggcaaggcc ggcggcgccg cccaccagtg tggccaccca ggcatagaac
     1201 agatatttga acatgatctg tccccgcttg tagcccaatg ccttcagtgt gccgatctgc
     1261 aggcgttctt cctccaccat gcgcgtcatg gtggtaaggg ccaccagcgc ggccaccagg
     1321 aaaaagaaaa tggggaacac cgtggtgatg gcctccagct tatccacatt ggaaaggtaa
     1381 gaggacgcgc tcacgtggga ggtgcggtcc agaatcatcc attccggaac ttccagctcc
     1441 gccagcttct gctccccgtc cgcgatctca gcctcggcgt cggccagctg ctgctctaca
     1501 tcttttttct gtttttcgta ttccgcctcg ccgtcttcca gctcggcttt gccgtcctcc
     1561 agctgggcca ggccgtcctc atactgcgcc ttggcggaat ccagctttga ttgtgccagc
     1621 tccagctgga gctgggccag gccgggggcc aggctcaggg ccgtttcgcc ggaggcaatc
     1681 tgcttttccg cctccaggac ctgttttttg gcggccgcca gctgctgctg accgtcggcc
     1741 aattgctttt ctccctctgc gatctgcggc tctgcctcgg tgatctgtgc ttttgcggtg
     1801 tccaactgcg tgcgcgctgc ggcgagcgcc gcgctggacg tgtcgtaggc agcctgcgcc
     1861 gacgccagct gcgcgttggc ggtgtccagc gccgccttcg cgggggccac agcagtctgt
     1921 gccgccgaat attcggcctg tgccgcgttc agtgccgcgt ttgcggtatc cagctccacc
     1981 tgcgccgcgt cccgtgcatc aatggcggcc ttggcggcaa ccggctccgt ggccagccac
     2041 tcgtcctccg tggcgcagcc tgcggcgctc atgcccgcgt ccacctgtgc ctgcgccgcg
     2101 tcgcgttcgg attgggctgt gtttgcccgg gactgcgccg cctcataggc ggcctgcttg
     2161 ccggagaggt ccgccgccgc cgctgcggcg tcatacgttt cctgggctgc ggctgcggcc
     2221 tcccgcagtg cgggcagcgc ggccgccagt tccggcaggc gtgcttctcc ctcggcaaca
     2281 gtgggctcca gtgtttccac aagctgtttg gccgcggcaa gctgggcctt cgcgtcggcc
     2341 agctcttgct ttttgtcgtt cagcagggcc tcgttttcct cgatctgcgc tttggcgtcg
     2401 atcagcgcgg cctggcctgc cgcaagctcc tgctgcttct gtgtcagagt gccgggcaga
     2461 ctttccttat tggcctgcag ctctttttcg ccgctggcaa tctggttttt cgcgctgtcc
     2521 agctgcgctt gggcgtcctc cagctccgcg cgcccttcgt ccagctgctc ttctgcatcg
     2581 cccagctttt ccgcagcctc tgccttggcg tcttccagct cctgccgggc gtcggccagc
     2641 ttttgctcgg tatctgtttt gacttcgttg taacgggcgg tgcagcgcgc gccgctgacc
     2701 gcctccaccc ggtccgccac ggcgtccaca gcggcctggt attcgtccga ggcgctgtcc
     2761 agcgcctgcg cgccggccac agtggcgaag atctcgctgt aagcctcctg ggaaaagctc
     2821 tcttcgccca cgtacatttt cagggcgacg gtgccgtcgc cgatggtggc gctttcccgg
     2881 tccaccgaga aataatnnnn nnnnnngtgc gcacgatgcc cacgatcttg aactcggtat
     2941 ctgccagcgt gccgctcacg tcgccgttgg tggcggcgat ggtcagcgta ttgcccacgc
     3001 tcagcggtcc ggttccgtcg atgacgtttc cttccaccag cacacattcg ttgcgctgaa
     3061 tgggcaggcg gccctccacc acgtccaggc ggttcagata attttccggc tctttttcct
     3121 cgatttggcc cgtgggcagg ctgtgaatgc gtgtgacgat gttctttttt tcgcctgcgt
     3181 ccacgaacat gtccgtcata taggcgggca tcacctcgtc tacgccgtcc acggcggcga
     3241 tggctgccac gtcatcgtcc gtcaggccca gcgggctgac gatgcgcagg tcgtgcatgt
     3301 tctgccggtc aaagtattcg tcgatggtgg tacgcatatc gatgggcatg gccatcagcc
     3361 ccgacaggag cccaacgccc agcgccacga tggcggcgat ggcgatgaag cggctgaagg
     3421 tgcccttcat ggtgcgcagc acaactttcc ggtatgtttt tttcacagcc gcgtcaccac
     3481 tcgatctctt ccaccggggt ggggtgttcg ttcaccacat tggagatggc ctgcccgctt
     3541 ttgatgcgga tgacgcggtc ggccatggcg gtgagcgcgg agttgtgcgt gatgacgatg
     3601 acggtgcgcc ccgtgctgcg gcaggtgtcc tgcagcaggg ccagcacctg tttgccggtt
     3661 ttgtagtcca gcgcgcccgt gggttcgtca cacagaagaa tttttgggtt tttggccagc
     3721 gcgcgggcaa tggaaacgcg ctgctgctcg ccgcccgaaa gctgcgccgg gaagttgccc
     3781 atgcggtgct ccagccccac ctggcgcagc gtttcggccg caggcagcgg ctctttgcag
     3841 atctcgctgg cgagctccac gttttccagc gccgtcaggt tctgcaccag attgtagaac
     3901 tggaatacaa agcccacgtc ataccggcgg tattcggtga gctgccgctg attgtaggcg
     3961 ctcacctcct tgccgtccag ccagatatgt ccttcgtcgc aggtgtccat accgcccagc
     4021 atgttcagca cggtggtttt tcccgcgccc gaagggccga cgataatgca gaattcgcct
     4081 ttttcgatgt ggaaggtgac atggtcggct gcggcgatgc ggttttcacc catttggtaa
     4141 tatttgcaga cattgtcaaa ggtaacaaaa tgctccaaaa cggatacgct ccttcagcgc
     4201 ttgttaaggg acaggctgtc cgttaataaa cgcggtgttg attattttcg atgatttcat
     4261 tataataaga atcacaaaaa accgcaatca tcattgtgca aatttgtgtg gaaaaataga
     4321 cacattgcac agcatctgtc gatttttttg taaactttct atgacacgga gggaaaggcc
     4381 atggcagaga acaccacaga ccgccgcgtg cggaaaacgc gcgggcagct gcggcagggg
     4441 ctggtgcgtc tgatgcggga aaaaagcatt caggatatca cggtgaagga gctgtgcgag
     4501 gaatgcgata tcaaccgcgg tacgttttat ctgcattata cagacgtgta ccagcttctg
     4561 cacagcatcg aggacgagct gctggtggag ttcgagcgcg tgctggacgg cctgacggcg
     4621 cagtcggtgc tggcagcggc cacgcccagc ccggccatgt gcagcatgtt tgagctgctg
     4681 gccgacaatg cggacatgtg cctggtgctg ctgtgccaca acggcgatat ggcctttctg
     4741 gaaaaggtca aggatattgt gcgcctgcgc gtgctggacg aatggggcgc gcagttccgc
     4801 agcaacgggc gggacaccta tgattatgta tattccttca tcgtatccgg ctgcatcggc
     4861 atgctgcagt gctggctgga gaaagagatg cccatctccc cgcgggagat ggcagccatg
     4921 gtggagggcg tgctggtgaa gggcgtcacg gcgctgctgt aactgccccg ccatgccgga
     4981 aaacccctct cccgccgggt gctgaaacgg cacggctccc gcatatagat ggggaaaggg
     5041 gctgatgttt atgctgttcg cgctcacact cacgacgctt gcaggccttt ccacggggct
     5101 gggcgggctt gtggttctgc tgttcaaacg cccgcgggcg cggatgatgg cgttttccat
     5161 ggggtttgcc gggggcgtga tgcttacggt gtcgctctcc gacatgctgc cgcataccgt
     5221 cgagacctat tccgatacca tgggccggtt tccggcggcg ctggcctctg ccagcttgtg
     5281 cgtgatggga atgctgatcg ctctgctgct ggagcggtgc atccccgatg aaaaggagct
     5341 tgcggcccgc ccggacgggc atgcggcgcg tgggacgccg gccatcaatc ccggcgcgct
     5401 gcgcagcgct atggtgacaa cggccgccat cgtgctgcac aatctgcccg agggcatcct
     5461 cacgctgttt acaagctacg ccagcccaac gctgggcgca acgcttacgc tggccattgc
     5521 actgcacaat attccagagg gcatcgctat ctcggtgccc gtatactacg ccacaaacag
     5581 ccgggtgcgc ggcgcgcttt acgctctgct gtccggcctg gccgagcccg ccggtgcgct
     5641 gctggcgttc tttttgctgc ggagcttcat ctcgccgctg ttcctcaacg ggcttatcgc
     5701 gaccatcgcg ggcattatga tctatgtatc catcagcgag ctcatacccg agggcttttc
     5761 ctacggcagg cgcggctata ccgtgggggg gctggtggcg ggcatcctgg ccatgagcgt
     5821 gggcatttac cttgtttaat cgcccgccgc gtgctatact gtttgtaacc gtttcttcat
     5881 atttttgtga tataataacc ttaaagccgc cgctgccgcg gctgcgcgtg tgcgctgcac
     5941 acgcccctgt gctggcgcac agggctttga gaacattgaa ccatgcaaaa ccgcagcagg
//
LOCUS       DAWBSB010000139         5885 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_2506_length_5885_cov_4.552487, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000139 DAWBSB010000000
VERSION     DAWBSB010000139.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 5885)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5885)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5885
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2506_length_5885_cov_4.552487"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1467 a         1532 c         1854 g         1032 t
ORIGIN      
        1 gacccagcgt ctcctccatg acctagccag gaaggacgaa aaattgtcat gaacaagtat
       61 gaaaaactgc tgcaggccac agagcctgca aggaatttcc tgcaagaata ctgcgaccca
      121 atgtgcgaga tccgcataac gttcgaccgc gtggacatcc tgcgcgggga aatggggcta
      181 ccgttcgagg ctgcacggca ggagcccgga agcataatgc acgacgcggt tttcggcagc
      241 cacccgtaca gacaggtaga agaataatag ccgaaacagc cctttggggc tgtcgtgcgg
      301 gggctgaccg ccccgtgcct gatgatggca ggtcaaggca ggtgattttt ttggaagtta
      361 tgttgagtgc ccgagaagtt tcagagctta caggctggcc gcttcggacg gtgcgataca
      421 gagcgcaaac agggcgaatt ccggctgtga gcattctgaa cgacagaaac cgatcggaat
      481 acgcttttcc gctggcaaca cagtcaaacg acatccagct caaatactac gagcagcatg
      541 gcctgacgct gagcgctccg gagaaaacag cgctgcagaa agtgaagccg ctgtccatac
      601 agcgtccgct ggacagctac acggatgcgg aacgtgagaa aatcgggttt tggcaggatg
      661 tcgtagacca atggatgctc taccgcagcc agccgggaaa gcctctgagc gagcttgacg
      721 caaagttcgt ccggcacatg cgcgtggaaa accccggcat ggagctgagc gtggccacgc
      781 tgtaccggaa gaagaaagcg ctggatgagg gcgacatgga cagcgtgatc gacaggcgcg
      841 gcaaagcccg taagggcaaa acggacatgc cggaatatat aaaaaaggcg tttttgtact
      901 actacctcgt ggacggcgga gagggccatg cgtattccat caccaagtgt atggaataca
      961 cggaaaaatg ggcggagaaa aacgcggcga acgcgctgcc gctgccctcg tacagcagct
     1021 tttaccgcct ggcgatggac gtgccggagg ccgtgcgcat cctgatgcgc gagggtgacc
     1081 gcgcgtacta caacaaggca agcgtataca cacgccgcga ttacgaaagc atcgcaagca
     1141 acgactggtg ggtcggcgat acgtatacat gcgatacgct tacgctgggg ccggatggca
     1201 agacgcacag gccgtacctc accgcctggg tagacgtccg cagcggaatc ttcgtaggct
     1261 ggtatatcag cttcggaggc aacagcagcc agaactccat ctatgcgctg cggcgcgggt
     1321 gcctggcgta tgggatgccg aaccacaacg catatgtgga taacggccgc gagtacctga
     1381 cgttcgactt cggcggcaga ggtcatcggg ccaagaaggt gctggccaac ggtgaagcac
     1441 cgtttgagcc gaaaaccatt ctggactaca tgggcgtgga aatgaaaaac gccatcgtgc
     1501 agaactcccg cgccaagctg gtggaacgca gcttccgaaa cgtgaaagag catatcatgc
     1561 ggctgtttcc tacatatacg ggcggaagcc cggaggaaaa gccggaagcc ctgaaaaaag
     1621 cgctgcagcg cggcgctatc cccacggatg cagagttcgt gcagaaggtg gacctgctga
     1681 tcagcgggta tctgaactat gagccgtact acggttccgt acctgcggac aagggcaaac
     1741 gccgcatcga tgtgtacaac gagcatctgg agcatgtacg gcatgtagag ccggatgtgc
     1801 tgaatctgat gatgctgcgc acatcgaagc ccaaaaaagt ggaccgggag ggcgtgtacc
     1861 tgaacatccg gggcaaaaaa gtctggtaca actgtccgga gctgcacagc ctgtggcagg
     1921 aaaaaaaggt gtatctgcgg tacgatccgg acgacctttc cagcgtgcgc gtataccgtg
     1981 aggacggctc cttcatcctt acagctcccc ggtgcgatct ggaggcagcc tacggcgcta
     2041 cacaggaaga aattgcacgg cagcagcagc ttaaaaacaa gtacaaacgt gtggtgcagg
     2101 agctggcggc gacgctgctt ccggacgcgc cgcccgaaga agctgccgcg ctggtggccc
     2161 ggatggccga ggaaaatctg gccggaccga cagccaaacg cgatcctcga accgtggagc
     2221 tcgttcagct ggatgaagag ccgtgggcga tggctgtggg cgaagtaaat gtatttcgga
     2281 tgaaccaaaa ttcaggaggt atcgacgatg agtacgacta ttaaagtgta cgacgaagct
     2341 ctgcaccgcc aggtagagga atacatggcg gcgcacaagc tctcgcagga gcagatgggc
     2401 gtgaagcttg gccgaagcgg cgccgcaatc aaccagtacc tgagccgcaa gtacaagggc
     2461 agcgtggaaa atcttgaaaa ggcgttccgc gagttccttg cacaggaggg cgaggccgtt
     2521 gcaacgcaga agcaggcgga gccgtacaag ctggacgaag gctataagcc aacgagcatt
     2581 tccgaggatg tttaccagtg catccgcttc gctcagatca accgcactct ggtgatgctg
     2641 cacggcgacg ccggtgccgg gaaaacgaag gcagccgtca aatattaccg cgataacccg
     2701 cagagcacca tctacatccg tctggacccg agcatggccg gacttgcggg cgttggagag
     2761 ctgctgggcg cggcgctgga cctgcctgcc gtgagcagca gcaaacagat gtggcaggcc
     2821 atccgagcgc ggctgcgcgg gaccaacaag gtgatcattg tggatgaggc ccagcttctg
     2881 aaacgggcgc cgatggatga gctacgtatt ctgccggatg aggatgaagt aaacgagctt
     2941 cccggcaacg gcgtggtgct gatcggcaac agcgagctgt atgaacgcgt gaaaaaaggc
     3001 aaggtaacga cgcagacata cactcgtatc gggctgcagc gcgcatacag caccatgaag
     3061 ctcacgaatg aggatgtaaa gctgctgttc ccgatgtttg ccggagagga acatgccagg
     3121 gagctgaagc tgctggcggg cgtctgccgg agccagcaca gcatccgcac ggcaaaaaac
     3181 atcgtcaaga atgcgatccg caatgaggat atttcgtatg agggcctgcg aacagccgcc
     3241 gccagcacat cagtagggcg gctttaaggc agggttacag ggaggtttaa agggatgttt
     3301 aacaggatga aaacggtggt cacattcgcg gccggagccg tggcagggct gatgctggcg
     3361 gcatgcctga cggtgcaggg ctcccgcccc ggcgtattcg gcggcgaggc ccttatcctg
     3421 ccgctgatgg gcctgctgct gtatgtgggc tacgaaaccg ggagcctgac cgcgctggca
     3481 caggcggaac gacggcggcg caggcacagg gccgggccac cgtaccgggt ggagcatggg
     3541 gatgaataaa ggatgcagga tggtggtgcc ctgtgaccgg agctgccgcg ggcaaagctg
     3601 ctgcgggtgc tgcagctccc cgccgggctt gcaccggacg gggacgctgt ggcagcccta
     3661 tgatgagaaa tgctgccacc gcacgcagaa ggaccagccg ctggtctgca tgcatctgca
     3721 aggccggctg cggcgggagt ggatacagga tatagaggag gaagacacag atgatggaac
     3781 tgataagcaa catattcccg aagatatcga tgatgcttgg cgcgctggtc attgtatgcc
     3841 tgctgtgcgg ctcagcgctg gcggccgtcc ggtgggcatt ctataaatgg gatcggctga
     3901 tccgtatgca cgacggcata atgattctat ttgaataccg ggatcaccgc gatgagttcc
     3961 ttgcctggcg tgaagaacag aaaaaatagc cggggccatc tggccccgcc gtaatgcagc
     4021 cgcgccgatg ggcgcgccgg ttccaagccc ggaaaaatgc agaggacgga aacggaggtg
     4081 gaaacaacgg aaaaggacga agaaaaggcg tcggaaattc tggcccgcgc tgcgaagttg
     4141 ctgaacgacg ctcccaaggt atacgatgcg aatcttgagc tgatgagaga gcaggacgcc
     4201 ctgcagcagg atctgctgca taagctggaa atcgaaaatc ttacgcggga tgagcgtgcg
     4261 aagctggcta cagagctgcg tgactgccgc aggctccggc gtaagtacaa ggacgttgtg
     4321 gaggagctcg agcccgttgc agggtattgc ggcaccacag ccggcatgca ggcggtaaag
     4381 cagttgtccc ggtttgtggg cgagctgcgg aaagtggaaa actaccacca gaaccggcac
     4441 tacgtcccca ggtccgggcg catcaaagga gaaagtcagg atgctgagtg aacaggaaaa
     4501 atctgagatc cgggcgtcat acagaaacgc gatcgacccg cgccagcagg tgaaaatcct
     4561 gacgcagctg tatcttgtga gccgggagga aattctggac atactggggc cgctttccaa
     4621 gtccgcccgc ccaaaaccga gccggaaggg ccagccgaga cgcatctatg cgccggagtt
     4681 caaggcagag gcaatggagc gcctgagctc cggagaatct ttccggcggg tggctgagga
     4741 tatgagcgtc aatgtacgga ccatggctac gtgggcctac cagatgcgaa ggaaggagcg
     4801 agagaaaaat gccgaactgt aaattttgcg gcaagcccgt aatatccgcg cgcgtgatgc
     4861 acgcgcactg ctgggaacag aaagtcatgg agctgatgga aactgtctgt gacagctatt
     4921 gccgctggcc gcttgagtgc cggagctctg aagaactgga ggaaaatcac tgtaacgact
     4981 gcgtgatgat tcaggcactc aacctcgggc tgtgagtgtt ggaaaaggcc ccgcgtatgc
     5041 ggggccaagg ctcttcacgg gtaaaatatc cggataggcg attcatcggg atgcaaactg
     5101 ttccacattt cgacaatatc cagcggcatc aaccggaacc cctttttaga aatacataca
     5161 ccgttgccga ggtcggccag tgctttgtag agcaccacga ccgcaccgcg ttgcagtccg
     5221 gagatccact gacggatgac aagagcttga agatcggata cggcctgttg tttgagccaa
     5281 cctgtttgca tcgccgaatc accatcccgc tccacaacac gtcccataag aaggtcgtaa
     5341 acagccgggt caaagtcctt cccggcattc cagtcgtcca cgagcttctc aagcgtggtg
     5401 cgtgtctgtt ccgggtcgac ggcatacgct tcatggagta cgctttcgcc tgcgcattcc
     5461 tcaatcatgg cgctgagttc gtcaactgtc atggaatatc ccctcttttc tttttctttt
     5521 agtatacgat aaataatgcg cgctgtcgac ctcgggctgt agaaaaagga ccgccccgtc
     5581 cggagatacg gaccggacgg ggcgaggatg tggagaatat gaagcggagc gcttcacagc
     5641 ttttaagata ccataggcgg ggcggaaaat caacaaaaac cgacaaaaaa tcaacaagga
     5701 agtgaagaaa aaatgacggt acagcaatgg cgcagtctgc gccggaaccg aagatgcaca
     5761 tactgcgcac attcccatat cgtttacggc cgggatggga gcatctgttt ttgccgggca
     5821 aaggacaaag ctgtgtatga gggcctgccc cgctggttct gccaagtata cacggtggag
     5881 gagga
//
LOCUS       DAWBSB010000140         5869 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_2514_length_5869_cov_3.139147, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000140 DAWBSB010000000
VERSION     DAWBSB010000140.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 5869)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5869)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5869
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2514_length_5869_cov_3.139147"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1164..1185
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1151 a         1653 c         1855 g         1188 t
ORIGIN      
        1 gtaaaggccg gggtcgatgc ccatgacggc ggccatcagc aggatggtgg tgttgccgaa
       61 ccacatgaga aaattcatca ggcccaccag cccgcgcgtg cctgccacgc tggataaaaa
      121 gcggaacggc tcttccagaa agccggcgga aacaagcgcg gcgttgaccg gcccgttgtc
      181 ggaaaacaag gcgaaaaaca gcatggcaaa ggccgaggcc ataatgaggt tgggcatgta
      241 gatgaccgtt ttgaaaaagc cctggccgcg caggcgcagc cgcgtgtccg tgaaccaggc
      301 cgccagcagc agcgacacgg caatctgcgg cacaaagccg atgatccaca tcagcagcgt
      361 gttgccgaag tatttcggca aatcggtgcg gaacagctcg gcataattct taagccccac
      421 gaaattgggg ccgatctcct ttaggccgct gcggaaatat tcaaaaaaac tgtaatatac
      481 cgtggaggcc agcgggatga gctggaacac cgcaaaggcg atgaaaaacg gggcgatgaa
      541 aagatagccc catttggcgt agcggatgga gcggtgcttt cggcctgccg tgcgcgcttg
      601 catatcggtc acctgtcctt ttcagaggaa tgcggggcgg gcgacgcccg ccccgcgcgt
      661 ttgagccgtg gctgcgttta ttcgggccac actacttcgg taagttcagg gtatttttcc
      721 ccgatggacg tctggaaatt ggtcttggct gtttccaggt ccaccgcgcc gtcaaaataa
      781 tccttgaacg cgccctggaa gctttcgttc agcccctggt cgtaggggcc ggcgttgctc
      841 atgtcgatgt tgggcgcggc tgcggcgaac agcgcgatgt ggttctggcc gcccagaaaa
      901 tcggatttga agtcgctgga ggcaagctcc tccatggccg gggcgttgtt ggtgtaatcc
      961 tgcgtatcct cggtgatctg cttcatgatg gccttgtcgc aggtgaggct gcgcatgatg
     1021 tccgcaatgg cggcggtgtt gtcggtgccg gccgcggcgc agatccatgt gccgccccag
     1081 taatacggct gcgggccttc gcagacggcg taatcgccgt agatgccgtt gcccgcttcg
     1141 gctttgccgc cctcggcctc gggnnnnnnn nnnnnnnnnn nnnnntgccc agcagcgtga
     1201 aattgatgcc ccaggtggaa tagaagaacc cgaacacctt gccgtcgggg ccctggtccg
     1261 cggcccacac gtcgctccac aggctggatt tattgttgta gccgttgtcg gtgtaggtct
     1321 tggtctgttc cacccacttc atcaggtttt cgtccacagt tacggtggtg cccgtcaccc
     1381 atggggcgga gacgttgttg gagaaagtgc ggtagctgtc gtcatagccg gacagcatct
     1441 tgtagccctt cgcggcggcc tgttcggcca cagcgttgaa tttgtcccaa tcagacaggg
     1501 cctcctgcac ctgagcgggg tcgtcggtgc ccagtacgtc tttggcgatg ctgcgccggt
     1561 aggcgaacag gccgggggtg gcctgccagg tgacgccctt cagcttgccg ccggccgtgg
     1621 cgatgtcctg ggtgtacggg tactgcccgg acaggtctgc gtccgtcagg ccgatatcgc
     1681 cgcgtacgtc cagcgtgtag tcggaatcca catacttcag cgcgtaatcc gcttcgatga
     1741 ggaaaatatc gatcttgtcg tcggctgcgg cggaatcctg cgccagcagg gcctcgtcca
     1801 gcttgttctg gtaattgttg ttctcgttgg gattgatggt ccacttgacg atcgtgccgt
     1861 cgttgagcgt ggtggtggat ttgtcctccg ccacttcctt tacctcggga taatagtcgt
     1921 tgaaccggct ttggaactcg tcgttccagc accagatgtt gagcccggcg ccatcggcgg
     1981 gcgcgggttc gctgccggat gagggggcgg aggcggaaac ggaggcggga acactggaag
     2041 cgccggagcc gccgcaggcg gccagggaaa gcatcatgca caatgccata agggtcgcaa
     2101 gtgtacgttt catagattgt tttcctcctt gtaaagttcg gtcaggcggc cgtttggccg
     2161 cgcgatcaca aaatcagatg cgcctgaccg atgctccttc caacaggctg ccggggatga
     2221 ggatgctttc cggcagggtg gttttggggt gctcgatcag gctgacgaga cggtccgccg
     2281 cccagtagcc gatggcctgg gtgtcctggt ggtaagtcgt cagactgggg atcatcattt
     2341 tggcgagagg gatgccgtca taaccgacgg cggagatgtc ttccggcacg cggaggcccg
     2401 cccgggcgat ggcggcgagg ccgcccagag ccgacacgtc atcggtgaaa aagatgcatg
     2461 tgggccgctg cggcagcgcc agcagctcgg cggtgatgcg ttcgcagctg tcgcagtcgt
     2521 ggtaagcgcc ttcgcgcaca taggcgtcgg gcacctgcag ccccaggtcg tagcaggtct
     2581 tgtaaaagct ggcgcagcgg ctttcggtga cggccgtgcg ttcgccgtgg atgaaggcaa
     2641 tgttcctgtg ccccataccg aacacatggt tcaccagcgt ttgaacgccc ttgacgttgt
     2701 ccgaaacgat ggaagtacgg ttgttgaaca tgtggtccac tgtcacgacg ggcagctcgc
     2761 tctgcaccag ttccagcacc tgcgggtcca gaaagttcac gcataccaca catacgccgt
     2821 ccacgccgcg gtagcggcag tgctccaggt aagacgagcg ccgcttgccg acattgcggt
     2881 tgatgaaggt gacatcgtag ccgcaggcct cggcctgcgc gcggaagctc tccagaatgc
     2941 gcgaaaaata ttcgtgcccc aggccgctgt gctgcgggtc ctcgtacaga acgcccagat
     3001 tgtatgtacg gttcgtcttg agcgcacggg ccgcggaatt gggctggtag cccatttcgg
     3061 ccgctttgcg gcatacggtc tcccgcgttt gagcgctgat gtcatggtgg ccgttgagcg
     3121 ctttgctcac tgtcgccacg gacacgccgc attcctttgc aatatcccga atcgaaatca
     3181 taacggtccg cctttccaga agttttgctc tgctaaatcg aaatcgtttt ctggcatcta
     3241 ttataccgaa aggcggcttg tttggcaaaa ctggatactt aatcgttttc gcaacttcaa
     3301 tataccgcaa tctcgcttta atttcaacag aaatcaagtg atttttaagc gaaatcacga
     3361 agtttggagc cttttccaat ttcgacaaag agtatttggc gattctatcc gaaaatatac
     3421 cttttatttt cgccaatgat acgaaaacat tttcgctctt gttctatacc tttatttaaa
     3481 tggaaaaaag actaaaaaac aagcgggatc cgtactttac agcacttatc agcaaaaaag
     3541 gcttgctttt ttttgaaatg gcagtatgat aaaaatacag agcgcttgaa tgtatgcgga
     3601 cttaatcgtt ttcgcaaacg gggccgcagc atgctcccca aaatcgagta gcccgcgccg
     3661 cagggcacgg gtgtgaggag aataccttat gcgctatgga tactttgacg acgagaaccg
     3721 ggaatatgtg atcaccacac cgcgcacacc gctgccgtgg atcaattatc tgggcagcga
     3781 ggattttttc agccttgttt ccaatacggc gggcggctac agcttttacc gtgacgcaaa
     3841 gctgctgcgt ctgacgcgct accgctacaa cagcgccccg gcggacggcg agggccaccg
     3901 gttttatatc cgcgacggcg cctgcgtatg gaacccgggc tggcagcccg tgcgcacgga
     3961 actggattcc tatcgctgcc gccacgggct gggctatacg gtcatcgagg gtgcgaaaaa
     4021 cggcatagag gcaaagcagg aaatgttcgt tccgctgggc gacgcatgtg aagtgatacg
     4081 cctgacgctg cacaacgccg gggcagagcc ccgcgcgctg cagctgttca gctatgtgga
     4141 gttctgcctg tggaacgcag tggatgattc caccaacttc cagcgcaact acagcaccgg
     4201 cgaggtggag gttgagggcg gagccatcta tcataaaacg gaataccgcg agcgccgcag
     4261 ccactatgcc gtgttcggcg tgaaccggac gcccgacggt ttcgacacga gccgggatgc
     4321 gtttctgggg ccgtaccgcg gcccggccgg gccgctggcg gtggaacggg gcgcgtgtac
     4381 gggcagcatc gcccacggct gggcgcctgt gggcgtgcat cagctgaatg tgacgctggc
     4441 cccgggcgaa cgccgcagct ttatttttgt attgggatat gtggaaaacc cgccccggga
     4501 aaaatgggcc gcgcccggcg tggtggaaaa aagccgcgcc cgcgccatgc tggcccgcta
     4561 tgccgcggac acgcaggttg acgccgcgct ggccgcgctg gccgcgtact ggaatgggct
     4621 gctggcgcgc ttttcgctgc gctccggcga cgaaaagctg gaccgtatgg tcaatatctg
     4681 gcaccagtac cagtgcatgg tgacgttcaa tatgagccgc agcgccagct attatgaaag
     4741 cggcaccggc cggggcatgg gctttcggga ttcctgccag gacctgctgg gcttcgtgca
     4801 tctggtgccg ggccgggcgc ggcagcgcat tctggacatc gccgccaccc agttcgcgga
     4861 cggcagctcc tatcaccagt atcagccgct gacgaaaaag ggcaatttgg acgtggggag
     4921 cggcttcaac gacgacccgc tgtggctcat cgcctgtgtg agcgcatacc tgcgggaaac
     4981 aggcgaccgt tccatcctgt acgaggaggt gccctttgac tgccgggagg aagacacggc
     5041 gccgctgatg gagcatctgc gccgcagcct gggctttacc gcttcgcaca aaggccctca
     5101 tggactgccg ctcatcggcc gggccgactg gaacgactgc ctgaatctga actgcttttc
     5161 cagcgaaccg ggcgagagct tccagaccac cggccccagc gaaggccctg tggcggagag
     5221 cgtgttcatt gcggggatgt ttgtaaaata cggcggggaa tacgcggaca tttgtgcgtg
     5281 ggaaggcctt tcgcaggaag cgcgggccgt gcgcgcacag gtggacgaaa tgacagcggc
     5341 catcctcggc ccgggctggg acggcgcctg gttccgccgc gcctatgacg caaacggccg
     5401 gccggtggga agcgccgaat gcgcggaggg caaaatttac attgaacccc agggcatgtg
     5461 cgtgatggcg ggcgtgggcc tttccgacgg ccgcgcgctt caggcgctgg agagcgtaag
     5521 gcggcatctg gacacagagt acggcatcct gctgctgcag cccgcataca cgcagtatca
     5581 tctggaactg ggggagatca gcagctatcc gccggggtat aaggagaacg cgggcatttt
     5641 ctgccacaac aacccatgga tcagctgcgc tgaatgtgcg gcggggcgcg gcggccgcgc
     5701 ctttgaggta taccgccgca cctgtccggc ctatcttgag gaaatcagcg agatccaccg
     5761 caccgagccc tatgtgtaca gccagatgat cgccgggcgg gacgcggccg cctttggcga
     5821 ggcgaaaaac agctggctga cgggtacggc cgcatggaca ttcgtcaac
//
LOCUS       DAWBSB010000141         5809 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_2549_length_5809_cov_3.097150, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000141 DAWBSB010000000
VERSION     DAWBSB010000141.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5809)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2549_length_5809_cov_3.097150"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1303..1312
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1255 a         1523 c         1949 g         1072 t
ORIGIN      
        1 aaaacacagc cgagggccgc gagggcatgg cgctggcgca gtatgtggcg ggcatgggct
       61 tctccaatgt gggcctgggc atcgtacaca gcatggcgca cggtctgggt gcgctgtatg
      121 atacgcctca cggtgttgcc aacgcgatca tcctgccggt cgtcatggaa tacaacgcgc
      181 cgtacacggg tgaaaaatac cgggagatcg cccgcgccat gggggtggaa ggcgtggacg
      241 caatgacgca ggaggaatac cgcaaggccg cctgcgacgc tgtgcgcaag ctgggacagg
      301 atgtgggcat tcccgcagac ctgaaagcca ttgtgaagga cgaggacgtg cagttccttg
      361 ccgagagcgc ctttgcggat gcgtgccgtc cgggcaaccc acgcgatacc agtgtggagg
      421 aaattgccgc gctgtaccgc agtatgatgt aaaacagagc cgccgctgcg gctgccggcc
      481 cggggcatac gctccgggct gtttttttgt cgaaaccgta aaagaaacgt cgtttctgtt
      541 tgactttggg cgtgttccgg gattatactg aaagagttga agccctgcgc cttgggaggg
      601 gtgcgggagg cgttcaaaac aaaacggaaa gggacagggt gctatgaagt tttttatcga
      661 cacagcaaac gtggaggaca tcaaaaaagc caacgatatg ggcgttatct gcggcgtgac
      721 gacgaacccc agcctgattg caaaagaggg gcgggatttc ggtgaggtca tccgtgagat
      781 cgcgtccatt gtggacggcc ccatctccgg cgaggtaaag gccaccaccg aggacgcggc
      841 gggcatgatc gcggaaggtc gtgagatcgc ggcgatccat ccgaacatgg tggtgaagat
      901 tcccatgacg gtggagggcc tgaaagcgac caaggtgctt tccgcagagg gcatcaagtg
      961 caatgtgacg ctgatcttca gcgcgaacca ggcgcttctg gctgcgcgcg cgggcgcggc
     1021 gtacgtcagc ccgttcctgg gccgtctgga cgacatcagc atgccgggca ttgatttggt
     1081 gcgcaccatt gcggatatgt tcgccatgta tgacgatatc catacggaga tcattgcggc
     1141 cagtgtgcgc aaccctgtgc atgtgacgga ctgcgcgctg gcgggcgcgg atatcgccac
     1201 ggtaccgtac agtgtcatcg agcagatgac aaagcatccg ctgacggatg cgggcatcgc
     1261 aaaattccgg gccgactacg aaaaggtgtt cggaaaataa ttnnnnnnnn nnaccgaaag
     1321 gcagaaaaaa cgtggctcac aggcgcatat cctgctacaa taaagaaaat aagtgcggat
     1381 ggggaaaagg ggctgtccgc gaaggaggaa gcatttatgc tgcaggaact gaaaatgaaa
     1441 agtctgaagg gacacgctgc gtcgatcatc atcgcgctgg ctctggcggc ggggctgctg
     1501 tgggggacgg gcttcggcgc attcaaagcc atcggcggcc cgaagccgtt ggactctttg
     1561 gatgtgtccg agctgaaagg gcaatacgtg gaggcggagg tatacctgat ctatgactgg
     1621 tatgcctata cagagcgcac caatacatcc acaaaccgct ctactgtcac agagaaggaa
     1681 tacattattc cggtagggga tgcggaatat atgggccttg cggtggacgc cggatatatc
     1741 agcacagcgg atgatttgct ggatgcatcc tatgaactgc tgacaggcgc cagccagacg
     1801 ccaggggaaa gctttacggt gcgggggacc attctgccaa tgtcgggaga gtcgctggat
     1861 ttttaccatc aggtggtcgg ctatgacgac tggacggccg aggagcagca gatgttcctg
     1921 ccgttggtgc tgaaagcgga ttacctgggc agcatggacc aatccggtac ctggctgctc
     1981 acggcaggcg cgggcgtggc gctgtttgcg gcggtctgga tgctggcggg tgcgctgacg
     2041 gggcggtacc aaaaacgcat ccgggcttac tgcaaggcaa gcgagagtcc ggaagcgatg
     2101 ctggaacagc tggaaacgtt ctaccagagc actgagcctg tgaacggagt gcgtacaggc
     2161 cggtggatga tgttcgagac cggaggcaaa acgacagtac tggacgctga aaatgtggcg
     2221 tgggcgtata tgcgcacggt gcagcaccgc accaacggca ttcccacggg caagacgcac
     2281 ggcctggtgg tgcgcacgcg ggataagaag atgtatgaga tatccatgaa aaaacaggac
     2341 atggtatacg agacgctgga cgcagtgcag cgcgcgatgc cgcatgtggt ggtgggttat
     2401 acggcccggc tggagcagat ttacaatacg cgcctggagg attttgtgcg cctgccttac
     2461 gacgaagcgc tgcgcgccga gcttttcggc acgcaggctg ccgcggagcc ttatgattcc
     2521 tgaaaaaaga aagatgttcg gctgtacggc ccctccggag aggggccgta tattttttgc
     2581 aaaatcggtt gacaataata ctaaataggt atagtatgaa tatagacccc caccccgggg
     2641 gagaggattt gagagaggga acaagatggt aaaacaaaaa tacaacgtca cgggcatgac
     2701 atgctcggcg tgcagcgccc atgtggaaaa ggcggtacgc ggcgtgcagg gcgtgtcgga
     2761 agtgtcggtg aacctgctga cgaacagtat ggtggtagag agcgatgcgc cgctggagca
     2821 ggatgtgatt gtcaaggcgg tggagcatgc aggctacggc gcgtcgcctg cagacggccg
     2881 ggcggcgggc cccgccgcaa agcagggggg cgcgcccgtg cagaacccca tgcaggcgca
     2941 gctgaaaaac atgcgcatgc gcctgtgggt ctcgtttgcc ttcctggcgc cgctgatgta
     3001 tgtttccatg ggcagcatgg ccggccttcc tctgccggac tttttgacag ggcatgaaaa
     3061 cgccgtgggc tttgccctta cacagctttt gctgactctg cctgtggtgt atgtcaaccg
     3121 caagtattac gaggtggggt tcaagacgct gctccgggga agcccgaata tggacagcct
     3181 gatcgccatc ggctccactg cggcgctggt atacggcgtg tttgccatct accgcatcgg
     3241 ctatggcctg ggcgtggggg accatgcgct ggtgtcccaa tatcacatgg atctttattt
     3301 cgaatcggcg gcgatgatcc tcgcgctcat cacgctgggc aaatatctgg agaccaggag
     3361 caagggcaag accagcgagg cgatcaccaa gctgatggac cttgcgccca aaacagccgc
     3421 cgtggagcgc ggcggggtgg agacggtgat ccctgtggag gaggtcgttg tgggcgatac
     3481 cgtgctggtg cggccgggcc agagcgtgcc cgtggacggc gtgatccttg aaggcaacac
     3541 gagcatcgac gaggcggcca tcacaggcga gagcatcccc gtggaaaagg agccgggcag
     3601 caccgtgatc gcggccacca tcaataaagc gggatttttc aagttccgtg ccaccaaggt
     3661 gggcgcggat accacgctgg cccagatcat ccgtctggta gaggacgcaa gctccagtaa
     3721 ggcgcccatt gcaaagctgg cggacaaaat ttccggcgtg ttcgtgccgg tggtcatcgg
     3781 cattgctctg gcggcctcca ttggctggct tctcgccggg cagacgccgg aatttgcggt
     3841 ctccatcggt atctccgtgc tggtcatttc ctgtccctgc gcgctgggtc tggccacgcc
     3901 cgtggccatc atggtgggca cgggaaaagg ggcggaaaac ggaatcctca tcaaatccgg
     3961 cgaggcgttg gagacggcgc acagcatcaa cgccatcgtg cttgacaaaa ctggtacaat
     4021 aacagaagga aagcctgccg ttacggacat tctgcccgtc ggcgcgttgg cggagaatga
     4081 actgcttgcg ctggccgccg gcctggagcg gcaaagcgaa catccgttgg cacaggccgt
     4141 gctggagtgc gccgcccggc gcggcgtgca gccctatgag gtgacggatt tcaaggcggt
     4201 gttcggcaag ggcatcgaag ggcggtatgc gcaggagctt tatttcgccg ggaatgaagc
     4261 catgctggcg gagcgcggta tggcgctgcc tgcccctgtg aagcagaagc tggacgcgct
     4321 ggccggcgaa gggaagaccc cgctgctgtt ctgcggcgaa aagacggtgc ttggcgttat
     4381 cgccgtagcg gacaccgtca agccttccag cgccgcagcc attgcggact taaagcgtat
     4441 gggcattgat gtgacgatgc tgacgggcga caaccggcgc actgcggagg ccatccgccg
     4501 tcagttggac atcccccatg tcattgccga agtgctgccg caggaaaagg agcagcatgt
     4561 agcggctctg cagcagcagg ggaaaaccgt cgctatggtg ggagacggca tcaacgacgc
     4621 gccggcgctg gcccgcgcgg atgtggggat cgccatcggc gcgggcacgg acgttgccat
     4681 cgaaagcgcc gatgtggtgc tgatgaaaag cgaccttgcc gacgcggtca cggccatccg
     4741 gctgtccaag gccgttatcc gcaacatcaa ggagaacctg ttctgggctt tcttttacaa
     4801 cacggtgggc atccccgtcg cggcggggct tttgtacctg ccgttccatc tgcggctgaa
     4861 cccgatgatc ggcgccgcgg ccatgagcct ttccagcgtg tgcgtcgtaa tgaatgcact
     4921 gcggctcaag cgtttcaaag cccatccggc gcatgccgcc gcggagcagg gggcgtccct
     4981 ttcgcccatg cccgcccagc cgcgcgccga acggatagaa ttgcctcaaa atgagaatat
     5041 caacaaaaac gacagggagg aaaaacaaat gaagaccatt acactgatta tcgaaggtat
     5101 gatgtgccag aactgccgca gccatgtgga caaggcgctg aacgggattt cgggcgttgc
     5161 cgcgaatgta agcctggaag agaaaaaggc cgtatgtact gtggaggacg gcgtgagcgc
     5221 cgatacactg aaggccgccg tggaagatgc gggctacacc gtcatttcgg tggaataagc
     5281 cgtgcgggcg gataagcaga cggtcacgcg gctgctgaaa acggcgcgcg gccagttgga
     5341 cggcatcctt gccatggtgg agaacgaccg ttactgcgtg gacatttcca atcaggtgat
     5401 ggccacgcag tccatcctca ccaaggcgaa caaagagatc ttaaaagcgc atatcggcgg
     5461 ctgcgtgcag gaggcgtttg aaagcggaga cccggccctg caggcggaga agatggacga
     5521 agtgcttgcg cttgtcgata agctggggcg ctgacggatg cggggagaag aagcaaaagc
     5581 cgccgcggcg cgggcgtggc ggagcgacaa aaagctgtat ctcgcactgg gagcggtggg
     5641 cgcgtttctg ctggcgctgc gccttgtgat gcgcagccgc gccgcatcgg acttcatcgt
     5701 gcacaagatc gtgcagccat accaccgggc agtgggatgg ctgtgcggtt ttgcgccctt
     5761 ttctgtggcc gagctgtgct gggccgtgct ggttttgttc tgcatctgg
//
LOCUS       DAWBSB010000142         5670 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_2608_length_5670_cov_3.054319, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000142 DAWBSB010000000
VERSION     DAWBSB010000142.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 5670)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5670)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5670
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2608_length_5670_cov_3.054319"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    643..652
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3944..3953
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1179 a         1706 c         1595 g         1170 t
ORIGIN      
        1 catccttcgc atcaaaatcc gcatccgtga tcacgccgtc cttataagtg acggacagat
       61 cctccgtcca atcatgggac ggttccttgt actgggcggt atatgtgccg tccttgatgc
      121 tgccgctgcc gccgcaggcc accagagaaa gcgccagcac agccgccaga gccaaagtaa
      181 gcagtttctt catagggaat tacctcctaa ttcaatcgca aaactctgct tgtgaaagtt
      241 ttgtcatggt tagaatatca ttatctgcac tgtaagtcaa cagcaaaata gttacaattc
      301 ctattttttt actctgctat catcaatgcc cgtaaaacta tctttcccat cctgtgccca
      361 atcatgcaac acgcatgggt gtctgttcct tggcagcaac gcgcagcacc ggcgctttgg
      421 gcgcgggacg gaacaggttt ttctcagcct gatacgcggc ataagccagt gccgcacagg
      481 caggcgtcag cagcatttcc gcgcaaagtt ttccggcgcc ataggctcct acaaagcaga
      541 tcgcgcccaa taccaacaga acgaccgcca tacgcaaaaa aaagcttttc atttcctttt
      601 tcatcatgat tcctcttttc gcagcttgaa tcgctgaata ttnnnnnnnn nntttacttt
      661 gaccaccata agtagtaaaa tcaaatatag aatgatagat agaaatgagg catccgcatg
      721 tttgcacaac gtttgaaaga gctgcgcaag ctgaatgggc tctcccaggc ggcgctttcc
      781 agcctgctgg gcgtcacgca gcaggctgtc ggcaagtggg agaccgggcg ttccaccccg
      841 gacccacaga cgctcgcgcg cgttgccgct tactttaaag taagcaccga ttatttgctg
      901 ggctgtgctc ccgcccctat cacctccgcc gttgcggcag cggatggtcc ccgtgaattt
      961 cccataccca ttatcggcac agtgcgcgca ggttttggcg cgttcgcctt tgaagaggac
     1021 tacggcgagg aatacgcccg cgtaaaagac cccggcagtt atttttatct gttggtaaaa
     1081 ggcgacagca tggaaccccg catcagcgac ggtgaccttg ctctggtgca ccgccagccc
     1141 acgctcgaga acggagatct gggtgtcgtt attttcggag agggtgaggg aacgctgaaa
     1201 aaattcatcc ggcggggcaa caccatcatc ctgcagccct tcaacaatgt atatgcgccg
     1261 cagatcatca tgggtgaaga tctggaacac ctatatatcg ccggcaaggt tgtggagaca
     1321 cgtgcacgct ggtagaccgc tcctctttgt ctctcaggat atcagaatat ttgtcccata
     1381 atacgtactt ttggaggaaa tatgccagca actgaaattt acctgatacg ccacggccag
     1441 accgattaca accggcagtt ccgtctgcag ggccgcagcg atattccgct gagcaggctc
     1501 gggctcgccc aagcccgggc tgcgcacgaa gcattgcgcg gcgttcattt tgacgctgta
     1561 tacgcaagcc cgctgcggcg tgcggtagat accgcatgta tcgtctcggg ctggcccgaa
     1621 gaaaaaatag agctggaccc acgcctgatc gagattggtt tcggcatttg ggaaggcagc
     1681 gatttccgca cgcttggccc tgccggtacg gccttttttg aaacgccgca aaattatacg
     1741 ccgccgcagg gcggcgaaag cctggattcc gtgctgcgcc gcaccggagg ttttctggat
     1801 gcccttcaac aactgccgga agatcggcac gtccttgtcg ccagccatgg cgctgcactg
     1861 aacgcgctct acctgcagat caaaggactt cctctctccc tgttttggac gcatcgtttc
     1921 ggcaattgca gcattgtccg cctgggcctc tccggcggca ggctgtcggt tctggaggaa
     1981 tatccggagc cggtcgcccc cgcctgagcc cggaggggac cgcgcgccgg gagcatgctg
     2041 gccgcggaaa ttgcacaaaa gcgcgtgcaa tgatgcccct gtgtaaggaa aacctctttt
     2101 ttcttatttg aaatcccaca aagccctgtg aaaaaacacg tgtttcgtca gggctttctt
     2161 ttttacaccc tttttcaaaa ctgccgtata ccggcacgga ggaacgagca tggatgtgct
     2221 ggaaaaactg acgatcctga cggacagcgc aaaatatgac gtggcctgta cgtccagcgg
     2281 ctccgagcgg ccgggcgacg agaccggcct tggcagcact gtggccgccg ggctgtgcca
     2341 tgcctttgcg gctgacggcc ggtgcatcag cctgctgaag gtgctgctga gcaactgctg
     2401 cgtttacgac tgcaaatatt gtgtgaaccg tgtgaccaac gatgtgcccc gcgccgcctt
     2461 tacgccgcgc gaactggcgg atctgaccat cgggttctac cgccgcaatt acatcgaagg
     2521 gctgttcctc tccagtgcgg tgctgcgcag tccggactac acgatggaac agatgatcgg
     2581 cgtgctggag ctgctgcggg gcgagtaccg ctttcgcggt tatatccacg caaaagccat
     2641 tcccggagcg gacccggcgc tggtggcgcg gctcggcctg ctggcggacc gcatcagcat
     2701 caatatcgaa cttcccagtg agcggagcct ttcgctgctg gctccagaca agagcaaacg
     2761 cgccattctt gcccccatga agcaggtgag ccaaagcgtc acgcaaagca aagctgagct
     2821 gatgcgctac cgcaaagccc cgcagttcgc gcccgcgggg cagagcacgc agatgatcat
     2881 cggcgcaagc cccgaaacgg attatcagat tttgcggctg accgaggggc tttaccaaaa
     2941 atacagcctg aagcgcgtat ttttctccgc ctacattccg gtggccgagg attcgctgct
     3001 gcccgcgctg gacaccaaac ccccgctgct gcgcgagcac cggctgtatc aggccgactg
     3061 gctgctgcgc ttctaccagt tttccgcctc cgagatcctc gacgagcagc atccggactt
     3121 caatccctac ctggacccca aatgcaactg ggccgtccat aacatgcatt tgttccctgt
     3181 ggacataaac cgctgttcct acgaaatgct gctgcgggtt ccgggcatcg gcgtaaaaag
     3241 cgcccagcgc attcgggtgg cccggcgcag cgggcagctg gaccttgccg acctgaagcg
     3301 tatcggcgtg gtgctcaaac gggcccagta tttcatcacc tgcaaaggct tttcccgtgg
     3361 cgtgactgcc gggccggagg ccaccgtgcg cgccctgctc gaccccgccg cctttggcgt
     3421 tggcacggaa cagctgagcc tgtttgccgc gcccgccgta caggagctgg cggcacaggg
     3481 aatgcgccct gcgtcggccg cgcgggaagt atgtgaggag gcggtctcgt gcctggcacg
     3541 tcagatgtga tctacctgta cgacggcagc tttgaagggc ttttgtgctg cgtgcacgaa
     3601 agcgtatata cccatgagct tcctgtggat atccagcccg aagaagcggc ccagcctacg
     3661 ctgttccgcc agaaatatat cgcagcagac gaggaaaagg ctgcgcgcgt ctacgattcc
     3721 attccccgga agatctcgcc cgacgcggcc gctctggtgc agtgcgtatt tttgagctgt
     3781 atgccgggca aggagctggc gattctccgc ttccttctgc tgggctatcg gcgcggacgg
     3841 cagacgatgt atctgttgtc ccacactgct gttcagccga tgctggccgc gcggcagaat
     3901 ctgctgaacg aagcccatct gctcaaggaa ttcctgcgtt tttnnnnnnn nnncatttct
     3961 gctcccgcct gaagaatgaa acctttctga tcttcgaccg tactcacaaa gccgcactgg
     4021 tctggcagga ccgcaaagcg cggatcatct ccctggatac gctggagctg ccgcccgcca
     4081 ccgccgagga gctgcgctac cgcgtccttt ggaagcgatt ttacaagacc atcgccatcg
     4141 ctgcgcggga aaacccccgc tgccgcatga cgcactgtcc caaaagatac tgggaaaaca
     4201 tgctggagat ggaggatgag ctgcggcgca cgccggacag tctggatgcc ttgccggccg
     4261 ccgcgcccgc cttgctccca caaaaggagg gacattatgg agcccaatat tccttccccc
     4321 gtttgccgta aagacggcgt cgagatacac gccgccgtcc ctaaggacat cacagaagcc
     4381 ttttccgtca ttgcggtacg ggaaaacggc gcctggcttc tggtgcgcca cagcgcgcga
     4441 aaaacctggg agctccccgg cggacaccgg gagccggacg aaacaccgct ggaggccgca
     4501 tgccgtgaac tatacgagga aacgggcgct ttgaggttcc gtctgtgcgc ctgcggctgc
     4561 tatagcgtca cacagggcgg acagacgagc tggggtgcac tgtttctggc ggaggccatc
     4621 acgcgcggcc ctctgcccga aagcgaaatc gcgcaagtac gtgcctttgc cgcactgccc
     4681 agcgcgctca cctacccgac gatacagccc gtactccacg cttgtgcgga gaaatacctg
     4741 cgcggcggcg cgctggaaaa cgcacctctc ttccgcgcgc ccgtacggaa gggggaacgc
     4801 ccgtgaacga tgctctctat cttcgcgccg tggggattgc cgccgcggtg cagcctgatt
     4861 attccttttc cctgccggct gttcgggcgc tggcccgtat ggggcgcctg cggctggaaa
     4921 aacctgtcac gtttttggtg ggagaaaacg gcacgggcaa atccacgctg ctggaggcca
     4981 tcgcggcggc atggggtttc aatcccgagg gcggcacgcg gaatttccgc ttctctaccg
     5041 ccagaacgca cgcggagctg taccgctcgc tggtgctgca gcgcggcccg cgccgccccc
     5101 gggatggttt tttccttcgg gcagaaagct tttacaatgt tgccacagag atcgaccgtc
     5161 tggatgagat tgcgccgctg aaaggcgcct acggcggccg ttcgcttcat gagcagtccc
     5221 acggtgaaag ctttctcgcg cttgtactca accgtttttc aggcagcggc ctgtacctgc
     5281 tggacgaacc ggaggccgct ctttcgcccg cgcgtcagct tgcgcttctg gctgctctgg
     5341 acggactggt ccgcaaaaat tcccagtttc tcatcgccac ccattccccg ctgcttctgg
     5401 cgtatccgaa cgccgaaatt tttgttttga gcgaacgggg catcgaaaaa acgccctacc
     5461 ggcaaacaga gccttacctt ttgaccaaac gctttctgga ggcacctgaa cgtatgctga
     5521 acgagctgct cggaacggag gaggaagcat tcgaggccag ataagtccga cgtccgcaga
     5581 cataaaacag acggacaaga aagaatagat acagatcgag gaaaagctgg cggcggccaa
     5641 gcaatgcagc tttgcgaagc aaaatacagg
//
LOCUS       DAWBSB010000143         5667 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_2611_length_5667_cov_2.023521, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000143 DAWBSB010000000
VERSION     DAWBSB010000143.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 5667)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5667)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5667
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2611_length_5667_cov_2.023521"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2615..2624
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2937..2946
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3954..3963
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4424..4433
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5176..5185
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1267 a         1732 c         1441 g         1177 t
ORIGIN      
        1 taatgatatc cgtcctttac tgttaagcgt tacgcccaaa tgcgtacaga taaaataatt
       61 ataacacaaa acaggcggcg ggcaaagccc tgccgcctgt ttttattgtt tttttcacaa
      121 acgcaccgga taaaactcaa cctttcagat cttccggaag cgtcaggccg atggcttcac
      181 aattcgcctc gttgacagca aactccagat cctccacaga ggcgaagcct acgggaaccg
      241 tgcccacgtc tttgccgtcc accaaaattt ctaccgcctg agcagcagcc agtttaccaa
      301 gattatagta gttgatgcca taagtggccg tgccgccgga atccaccata ccgctctcgc
      361 cgcagatcac gggaattttg gcattctccg caaccagcgc tgcagtgggc atgttttcgg
      421 caaacagatt gtctgtggga acgtacagtg cgtctacctc gccgacagcc tttgtggtga
      481 cgggctgaat ctcattgacg tcggcggcgg tatactcgtt caccgtcagg ccgcgcgcct
      541 caaaggcggc tgttgccagg cccgcctgca gaatggagtt atcctctgcc gagcagtaca
      601 gaataccaac cgttttcgcc tcgggcacca gctttaccaa aaggtcggcc tgttcctcga
      661 tggggttcat atcagatgtg ccggatacgt ttccgcccgg gacctcgtcg ctgttcacca
      721 agccggcgct cacaaaatcc gtcactgccg tggccagaat ggggatctct gtggttgcct
      781 gtgcggcggc ctgcgcggca ggcgtagcta cagccaggat cagatctact ttatcattga
      841 caaacttagt ggcaatggtc gcgcaattgg gaatctcgcc ttgcgcattc tgcacgtcga
      901 tattttcccg gttgaagccc gcttccacca gcgcgtcgat aaaaccgtcg cgggcggcgt
      961 caagagccac atgctccgtc agctgaatga cgccgatctt gcagttggcc gggtccttgc
     1021 cggcggcagc aacagaagct gccgcagagg atgctgcgtc ggatgttccc ggttctgagg
     1081 cggccgccga agacgatatg gagccgccgc acgccgcaag gcccagcgcc accacggcgg
     1141 caaggcaaag tgcaaataac tttttcattt ctttccctcc ataggcaccg gaaaatacga
     1201 tgtgtaccgt atttttctct gatgctttat tgtgctaaag tatatgacaa aaagaggcgt
     1261 gaaaacaata gcggaatgct actaaattca cgcctgaata ttgtatattt ttgtatatcg
     1321 atacaacaaa gcggaagaaa cagcctgcaa tgccagccgc gcagagctta cagcggcagc
     1381 tccgcaagat attctcccat tttgtcaagt ccggcggcaa tcacttccgg cgtattggcg
     1441 tagccgatac gaagataccc ctcccggtcc attacactgc ccggtacgag cagcacgcct
     1501 ttttccgcca gcagcccccg gcagaaatct gcgctgggca tgtcgaactc catcttcaaa
     1561 aaggcagtcg tgcccgcacg cggctttaca aagctcaact taggctgtcc tttcagccac
     1621 cgctccaaaa cggcaagatt tccggtgacg atggcgcggt tgcgcgcgat gaccgcctcc
     1681 ttgtgctcca gcgctatccc ggccagatat tcatcgatca taccgcagct gatggtactg
     1741 taatcccgat ggcgggatac ttgctccaac agcgcttttg gcccggcgat ccagcccaga
     1801 cgaagcccgg ccaatgaaaa ggtctttgac atactgcctg tggaaattcc tttttcataa
     1861 aggtctgcca cagaggccgt gaacggctcg ccttcgtggt tcaggccgcg gtatacctca
     1921 tcgcacagca accatgcatc aacagaacgg gcgatcccaa cgatttccag cagaaattcc
     1981 cggtccatca gggcgcctgt agggttgttg gggttgttga cacagatgag ccttgtattt
     2041 ttattcacca tgccgcgcag ttcgtccagg tcgggcaaaa atgcatttcc gctgcgcagg
     2101 ggcaggatgt gaacttcagc acccagcgcc tccggaatgg aataatgctg ctgatacgtg
     2161 ggcagcacgg agaccacctc gtcccccggc ccggccagtg cttcaatcac aagatgattc
     2221 gcaccgatag ccccgtgcgc cgtaatgaca ttttccggcg tctgtttttc atacagtccg
     2281 gcaatgagcg tccgcaggcg cgggctgcct tcgatcgcgc cgtaggtcag cttcatgccg
     2341 aggatatcgg ccacagcctg ctcacggcag ccgccaagct ccaaaagctg ctccacggtc
     2401 agggattcca cacaggtctc cgccaaatta taagtacagt cgttttcata ggcgttcatc
     2461 cacatctcaa cgccaaagtc cttgattttc attactttcc gatcccctgt ctgattgata
     2521 atatcttcac tgcggcgcgc cgaaaatact ggccacagca gtcatggatt ctttcagatc
     2581 cgcccggtaa tccggcgcct cttccgcaag actgnnnnnn nnnngtggag tgaacaaacc
     2641 gtccctctcc cagatacatc gccacgtggc cggggaaaaa cagcagatcg cccggctttg
     2701 ctgcttcaaa tgaaatttca tgcagcgcaa accccggctt gatggcagca tccctgtaaa
     2761 taatcacacc gttgagcaga taggccatgg agacaaggcc gctgcaatcg atgcccaacg
     2821 gcgttttacc accccagcgg tattgtgcgc ccagataaga cagtgcggcg ccgcataagc
     2881 gctgccgcag ttcatcttcc ccaacgcttt cccacgcggg agccggctga gccagannnn
     2941 nnnnnntttg gtgtagccga gacgtccgtc cgcaagacga acgggctgcc actggttttc
     3001 atccgggccg ccctccgggc agagaaaagc tcccctcggc atgctctgca ggcagcctcc
     3061 ctgtaccttc ggctcgttca gcacatcgat ataaggtttt tgcactacca gctttggcac
     3121 cgactgccat tgtgcggcaa gcgcgccgtc ctccagaagg cagcgcgcgg gcgtccaacc
     3181 ctcatagcgg taatgggtac gcacacggca atacatccct tcccgcgcca gcacctccaa
     3241 taccatacca taaagcgctt catctgccag ctcacacaac ggctccggct tcgtatacaa
     3301 aggggcgatg tcagaaacca caacagcaaa actcattgcg tatcctcctg ggaataataa
     3361 tcgaaaacca acttcgaaac gcgtcccgcc agaggctcgt caccgtcaat atccggtgcg
     3421 tcccaaatga gtacggcgat gaaatacggc cggcccggaa gctcgaacac tcccgcatca
     3481 tggctcagac ggtccaggcc acccgtcttg tgggcacagg gcacgtcctg ccaaatgtaa
     3541 cgggtgagca tccgcgtatc gcgctgacgg cgcaggatcg agcgcgccag cgcgcaaagc
     3601 tctggcgtga gcactgtttc gtcatccagc gcacggaaca cgcgcagcag gtcctccgcg
     3661 ctggtataat tattgcgccc ggctgatacc gcatcccaat ccagcatttt gcgttccagc
     3721 acagtgtttt tcgtcccaac gctgcatgcc gccgcattta tacattcggc gcccagcagc
     3781 ccgatgagca cgttcgttgc cgtattgtcg gaaaggacga tcatccacac aagcagctcc
     3841 tccagcggat gcatgccggg accgtccaca aagacgcctg tgtcaggaag aatgtcttcc
     3901 gctgtgaccg ccacaggcgt gtccagcgcg agccttcccc gccgcacctc atcnnnnnnn
     3961 nnntcatcca gcgcggccag caggatgaac accttgatgg tagacgcgga gaccgccctc
     4021 ctgcccgcgc tccgttcata caacggcacg ccgccagcca acgggacagc cagcacgcac
     4081 acctgttcct gcgccgctgc aagctgcgca tccagcgcgg cgcaaagctc ttgcctggtc
     4141 atattcaata ctccaatacc cgcagcgtgc ggttcgttgc gtcaagattc acgtttaccc
     4201 ccagcggcag ggacatcgta ggcatgatat gcccgcactg cagcgccccc aggatcggct
     4261 tgccttcatc cagcagcaat tctgtgatga cctcacgcag cgtcaggctc tccgccgggt
     4321 caggtgctgc acaatccgtg aacgcaccca gcagaatacc cgcgcaggcg cgcagcttcc
     4381 ccgcctgctt cagcatgagc agcatgcggt caatacggta cggnnnnnnn nnngcgtttc
     4441 accgatatcc tcaatgaaca atattttatc ctttgtctcg atttcataag gcgttcccaa
     4501 agaggcggcg agcagcgaca ggttgccgcc cgtcaacacg ccgtggctca cgcccttggc
     4561 gatcgtatac agcggcatac cgtctggatt gaggatattc ggctcctgca cgccgaaaat
     4621 cgcgtttttc agtgaacgct cggtatattc atccaaacct gcataccatt ccgtagccgg
     4681 cataggcgtg tgataagtaa caaagccgca gcgctgattc agcatcagat gcaggatggt
     4741 gacatcactg tacccacaga acaccttggg cgtttttgca atagcagtcc aatcgatgcg
     4801 gtccatcagg cgctgcgcac catatcctcc gcggatgcag ataacgccat ggatgctcgc
     4861 gtccgaaaac gcattattaa aatctgccgc acgttcgcgg tcatatccgg ccagataacc
     4921 gtgccgcatg gtacaggacg gaaaaacgac aggctccagc ccaagtgcct gcacactggc
     4981 cacggcaggc tccaagcgcc cttctggaac agggccggaa ggcgcgatca gcgcgacacg
     5041 cgcgcctgta aacaacggtt ttggtataat catggcagct ctcctttgct tgttcagaat
     5101 ggttacgact tctccggcac atgctttcct ccgccggagg gcaaagccgc ggcaaacgga
     5161 aacatgtgta aaaacnnnnn nnnnnctgca tacagaaaca ccgaaagcgg acgctttgtc
     5221 ggctttcagc acggacgctc cgcgcgaaaa acgccgcgct gcgtccttca tgtcattcag
     5281 tttaaatcat tgtccggtat ttttcaagtt cttcctgatt tgcaagcaca aaatgtcccg
     5341 gttcgatctc cgtccatgtc ggagggtctg cctcataatg atgacaggac gggtcgtaca
     5401 cctccaggac cttgcgtttt tccgccgcag ggtccggcag cggaatagcg gaaagcagcg
     5461 ctctcgtata cggatgcagc ggatgtgcaa atagcttttc cgtactggaa agctctacga
     5521 tgcgtcccaa atgaatgacc gcgatgcggt cgcagatgaa acgcatcacc gagagatcat
     5581 gactgatgag cagataggtc agcccgcgcc gacgctgcag ctcggccatc aggttcagca
     5641 cctgagcacg gatggatacg tccaaag
//
LOCUS       DAWBSB010000144         5637 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_2626_length_5637_cov_3.516302, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000144 DAWBSB010000000
VERSION     DAWBSB010000144.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 5637)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5637)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5637
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2626_length_5637_cov_3.516302"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    307..316
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          967 a         1928 c         1688 g         1044 t
ORIGIN      
        1 atccgcggcg gcctcctgcg caggcgccgc ctgctcttct tcgggcgcgg cctgggtgtc
       61 cgcctccgct tcccgctgct ccgggacgcc ggacgccccg ccgctttctt ctgtttcagg
      121 cgctttttct tccgcctgcg cggtttcctc cgccgcgcgg gctgcgcggc ggcgctcgat
      181 ctcggctttc atctccgcgt ccgtgggagg cgtctcgccg gacagccagg agaactcata
      241 ggggacctgc ctgccgtctt cctttacaag cgccgtataa gtgacggtga gcggtttgtc
      301 tttgtannnn nnnnnngtac tttttgcgca gcgccagcca aatgcacagg gccgcgatga
      361 cggacaccgc cgcaatgacc tggctgatgc ggaagctgcc gaccatcagg ctgtcggtgc
      421 gcaggccctc cacaaagaag cgctccgcgc cgtaccacat cacatacatc aagatcagct
      481 cgccgtggaa tttgcggcgc ttcatatatg cccacagcag gaaaaagccc agcaggcacc
      541 agatggattc atacaaaaac gtagggtgca ccggcaggga cgggtccacc gcaacgccct
      601 gcgcggccag cgtttcctgc acgcttgaga ggtaattgca ggtgccttcg ctgtacatgc
      661 cccagggcag cgtggtgttg gtgccgaacg cctcctggtt gacaaagttt ccccagcggc
      721 cgatgccctg ccccagcaga aaacccatgc ccgccagatc gaacatcggc agaatgggga
      781 ccttgcgcca cctgcaggca aaataaccgc tgaggaacgc cgcgatgacc gcaccgtaaa
      841 tggcgacgcc gcccaggcgg atgtccagca tctcggcaaa gctggcgtat tcaaacgggg
      901 cagtggccac ataaaacgcg cgtccgccga tgacggcgca cacggtgccg atgaggatca
      961 catccaccat gcggtcggaa tcaatgccga aacgtcgcgc atggctgaat gcgaacacga
     1021 tggccagcgc caggcccgtg gcgatgagga tgccgtacca gtagatcggc atgccgaaca
     1081 cggtgaacgc cacgcggtcc agcgtaaatt ccagccccag gccggggaac tgcacatgat
     1141 aagtcataaa aagggtcgct ccttcctgat atgcggggcg gcctgtgccg cgcccgcagc
     1201 ccgcgccgta ttactgcgcc gggcggatga tgacggtagc gctgcgttct gtgcacagca
     1261 tggcgcgcag cgcgccgttc acgtccgaaa gcgtaacgga ggccagcgtc tcgatctcgt
     1321 cggcgggcgt tcggccccgg aaaaacgacg atgccagcgc gccggccacg tcctcaatgt
     1381 tctccaggtc ctgcaccagc tcgccataaa gctggttttt gcacagcgtg aacagttcct
     1441 cgtccacgcc ctcgcgcttc aaacgggcga tctcgtccag cagcaggccg cgcaccgtct
     1501 ccggctccgg cgtctcgccg ccgaacagga acgaaagcgc ccccggcagg ctgagtatct
     1561 cgcccgagaa gcccgggctc accagcccct cgtcgtaaag cctgcggtac agcggcgtca
     1621 tgctgccgca gataagctcc gtgagcatgt cgcagatgat ctccgtcttg agcgcgtcgc
     1681 ccgcgggcgg cgtttcttta aagccaacgc ccagcagggg ctttgccacg gccatggaaa
     1741 actcgcggta cggctcggcc acgtccggcc cctcggcgga ggcgaggcgc tccaccgtgc
     1801 ccgtctttac ggggatgtcc gcgcgggcgc aggccgccag cacctcgtcc atcgtcacat
     1861 tgcccgccac ggccagcacc atgttctgcg ggcggtaaaa ggcgtccgtg caggcataca
     1921 aaatttcagg cgtgatctgc gcgatgctct ccacactgcc cgcgatgtcg tcccgcaccg
     1981 ggtgcgcggt gtacaggcac tcgtacatgg cgaacatcag ccgccagtcc ggcgcgtcgt
     2041 catactgctt gatctcctgc ccgatgatgc cctgttcctt ttccacggtt gccgccgtaa
     2101 aatacggccg ggagacaaag cccagcagaa tatccaaatt ttcggccacg ttgccggaag
     2161 ccgtaaaaat gtagcaggtc ttgtcgaagc tggtgtaggc gttggcgctg gcgcccgtgc
     2221 gggcatacag tgtgaacgcg tcgccctcct cgttctcaaa cattttgtgc tccagaaaat
     2281 gggcgatgcc cgcgggcagg tccacccgtt tgccgtccag ggcaaaagca cggtcgatgc
     2341 tgccgaactt cgtgccgtat accgcgtgta cgcccgtaaa gcccggcatc gtatgcacgc
     2401 gcaccgtcag gccgttttcc agcaccgtgc tttcaaacgc cgcggctctg cgcaccgcct
     2461 gcaaagaggg atcatgcatc tgcgcccgcc tccttcgtca aagtgtagca taccgaaagc
     2521 ctgaagctcg acagcacgcg gcgcacgtcg tccgccgtca cggcgtccac ctcggcggcc
     2581 acctgctccg gcgccttgtc cgcgccgcgc atgcgctcgg caaaatacca gccctccagg
     2641 ccctggagcg tatcgcccac ggcggcgagc tggtttttca gcgcgcgctt cgtgtcggcc
     2701 agctccccgt cctccaccgg cccggcgcac agcgcggcca gctctttcag gatggactcc
     2761 cgggccgccg cggcgttggc gtgctccacg ccgctgtcca cacacagcgc gccggtgagc
     2821 gaagtgtagg acgccgcgca gtaatagcac aggctctgct tttcgcgcac gttctggaac
     2881 aggcgcgagg tgggcgtacc gcccagcagc gccacggcca cccgcagcgc ggaaaggtcc
     2941 tgcggcgcaa agggctcgcc cggtgtgaac agcatgcaca gcttgccctg cacggtgggc
     3001 agcggttctt caaagctgcg cggctcttcg gcgggcatgg cgatgggcgc gggcagctcc
     3061 tccggggcgc ggcgcaggcc cgacagcgcc gtgcgcaggc gctgcgccac cgcgtccacg
     3121 tccgctccca gcacaaacac ctcgatctgc gcctgctcca ccatgcgcgc gtaagccctt
     3181 gtcaccgcgt cggccgtcag gccgtccacc tcgtccagat aaccgttgcg ctcaatgccg
     3241 gcgggggaat cgccgtaaaa cctgcgccgc gcctgccgga tgcaatagct gcgcttttcg
     3301 ttgatctcgc cctccagcag ctcgcggagc tgctcctttt caatggcgac ggcctcggcg
     3361 tccagcacgc cgtccacgcg gtacggctcg aacgccgtgc cgaaggcgat gtccgcgtat
     3421 tcgcggctga gcggctcgcc cgccagcgca aacgcgtccc ggatgcccga gagcgacacc
     3481 gtcagcacgc ggctctggcc gctcatggtg ccgtccacgg aaagggccgc tccgtacagg
     3541 cgggccagct ttttggaaag ctccgtcata tccgggcagc cctgatagcc ccgctccagc
     3601 accagcggca gcagggcctc ggccgtggcc cactcccggg cggcgggcca gatgaagttg
     3661 atggaaatgc ggcagcggtt gaatttttcc gccggaagca cgttgatgtg cgcgcccggc
     3721 gcaatggtaa tgcgttccat ttgatacgtc ctttttgttc aaatatccgc cgttccggcg
     3781 gtggagtgtt ccgcgcgggc ccgcccgcgc ctgtctgtgt cattccgtcc ggcccagata
     3841 ctcctccagg cacacgccct gggccgtgat cttctgcgcg tcctccacgc ccacatagcg
     3901 gtagtgccac ggctcgaagg aggttcctgt gatatcggtt ttgtctttca tatagcggag
     3961 gatgaagccg tattccgggg cgtgctccct gagccacacg gcccagtcgc gctcctccag
     4021 gctctccacc agggcggcgt tgatgtccgc cgcgcccacg atgtccacgg ccaggcccgt
     4081 ctcatgctcc gaggtgcccg gctcgttgcg ccactgcttg gtggccgcaa aggcctcttc
     4141 ctcggacatg ccctgattga tataatcctg tttcatgctg ttgaacagcg tggtctgctc
     4201 ctgtgtggaa cgataggccg aacgcgccac cagctcgatg ccgtctgcct ttgcggccgc
     4261 gaacatctgc tccagcggct ccttcacgcg ggcgtccata aactgctcgc cgttgcggct
     4321 gttgctgccc acgctggcaa gctccggtgc gtagccctcg ggcagcgggt tcgtggtgtt
     4381 cgtcaggatc aggctccaca gcgcggggtc tgcgggggcg ttggggtccg ccgccggctc
     4441 accggaggat gcgggcggcg cggaggccga aacgctctgc gccgcgctga cggacgaagc
     4501 ctgcccgccc cgcgcccagc ccgccgcggc ttttatcagg aagaacagcc ccaccagggc
     4561 tgccagcgcc aacgcaccca gcagcgccag aaaggcgtaa taccggcgca tggcacgcct
     4621 gcgcaggatc tctttctgcc ggcgccgctc cgcggccatg gcgcgctcct caggtgtcat
     4681 gggccggcgc cggcgcggcg gctgttggtt ctgcatattt tttctccttt ttccggtcac
     4741 actgataaaa cgagtatcgg cgcagaatat ctacgccagg cgggccacgc gcattttatg
     4801 cagcattccc cggccgcgac gaaaacaaaa gacaggcctt tcccggcctg cgccgcaaaa
     4861 atgcctgtct ttcattatac ccattccgct gcccgatgtc tacggcagcg gagtgttttt
     4921 cacgctttcc gcactctttt ttcagttcag gaacgcgtcg tggatggcgt tcaccgcacg
     4981 gtccgcgtcc gccttggcga tcagcaccga gatcttgatc tcgctggtgg aaatcatgcg
     5041 gatgttgatg tttgcctcaa acagcgcctc aaacatgcgg gacgccacgc cggaatggct
     5101 ctgcatgccc gcgcccacaa tgctcacctt cgccacgtcc ttgtccacaa caatgtccct
     5161 gcagccgaag cgctggcggt tttcctccag cagcgcaagc gctgtttcgg cgtcgcccag
     5221 gggcacggtg aaaataacgt ccttgtggcc ctccttgccg gaggactgca gaataatatc
     5281 cacgttgatc ttcttctgcg ccatcaggct gaacaccttg aacgaagcgc ccggaacgtc
     5341 gggcacctcc aaaatcgaaa tgacggcgac gtccttgtcc tttgcgacgc ccttgatcag
     5401 cataccttcc acgtctgtga cctccttcac gattgtaccg ggcagatcgg tgagcgacga
     5461 gcgcacctcc agattgaccg aatatttctt tgcaagctcc acgctgcggt tgttgagcac
     5521 ctgtgcgccc aggctggcaa gctccagcat ctcgtcgaag gtgatctcat ccagcttgcg
     5581 cgcgcgcggc accaggcgcg ggtccgcggt gtaaacgccg tccacgtccg tgtaaat
//
LOCUS       DAWBSB010000145         5635 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_2627_length_5635_cov_2.865591, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000145 DAWBSB010000000
VERSION     DAWBSB010000145.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 5635)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5635)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5635
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2627_length_5635_cov_2.865591"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4310..4319
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1282 a         1381 c         1659 g         1303 t
ORIGIN      
        1 cataaaaaca acaatggatc gatacgtgca tgtgaccgat gattctatgg agaacggcgt
       61 ccggcaattt gagaagcatc tgacggaaca aattgcctaa gtggcagaaa tggtgtcaaa
      121 atggtgtcaa cgctgcgata cggtacgctg caaccccttg aaaatactgg cttttttagg
      181 agatgtgagg aaaaatgaaa ttaggtatcg taggcctgcc gaatgtcggc aagagcacgc
      241 tgttcaacgc catcaccagc accaaaaatg ccgctgcggc gaactatccg ttctgcacca
      301 tcgacccgaa taccggcgtc gtgcccgtgc cggacgcccg gctggacaag ctggctaaaa
      361 tttgggatac caataaaaaa acgcccgcca tcgtggaatt tgtggacatc gccggtttgg
      421 tgaagggtgc gtccaaaggc gagggactgg gcaataaatt tttgggcaac atccgcgaat
      481 gcgacgccat cgtgcatgtg gtgcgctgct ttgaaggcac ggacattgtg catgtggaag
      541 gcaatgtgga cccggtgcgg gacattgaga ccattgatac agagctgatc ctgagcgacc
      601 tggaggtcgt ttccaaccgt gcggcgcgta tggccaaggc gggcaagacc ggagacaaaa
      661 aggcgctggc cagcgccgca tggctgagcg cgctggagga gcatctgggc gcgggcaaaa
      721 atgcgcgcac cttcccgctg gacgaggccg acgaggacca ggtgctgcag atgcgcgaaa
      781 tgggcctgtt gacggcaaag cctgtcatct atgcggccaa tatgagcgag gacgatctga
      841 tggagggaat gctgggcaat ccgcattacc agaccgtgaa aaagctggcc ctggacgaag
      901 gcgcggagtg catccccatt tgtgccaaaa ccgaggagga tatcgcggat tacacgcccg
      961 aggagaaaaa ggaattcctg gcggagatgg gcattgaggc cacgggcctg gacaacctca
     1021 tcaaggccag ctacggcctg ctgggactta tcagcttttt gacggacggt aaaaaggaat
     1081 gccgcgcctg gaccatccgc cgcggtacca aggcccctca ggccgcaggg aagatccatt
     1141 ccgattttga gcgcggcttc atccgcgcca gcgtggtgaa atacgacgac ctcgccgcct
     1201 gcggttacag catggccaac gtgaaggcca agggcctgca gcgcaccgag ggcaaggaat
     1261 atgtggtgca ggacggggac atcattgagt tcctgttcaa tgtgtgagga ggcaccgcta
     1321 tggaaaagat catcggcctg atgggtgcca tgcgggaaga aattgcgctt ttgtgtgagc
     1381 gtatgacaga caagggggag gaaaccgttg cgggcgtgac gttttacaca ggcctgctgc
     1441 agggacgccg tgtggcgctt tgctgcgcgg gcatcggcaa ggtaaacgcg gcctgcgcgg
     1501 cgcagcttct cattgcaaga ttcggcgcaa aagcgctggt attttcgggc attgcgggca
     1561 atatgacgga taagatcggc gtgggcgacg ttgtcatcgg cggcacgctg gtgtaccatg
     1621 acgcagaccg tgaaatgatt tcagatacgt tcccgtattt gaaggagttt gcggcagacc
     1681 ctgtgcttgt ggccgccgcc gagcaggcct gcacggatgc gggcgtaaaa tatattgtcg
     1741 gcaagatcgc cacaggcgac acctttgtgg gcgacagcgc caccaaaaac gccattgcgg
     1801 ctgattgtgc gccggactgt gtggagatgg agggcgcggc cgtggcccac gtggcggcaa
     1861 aatacggtgt tccctgcgtg gtgttgcgcg ccatgagcga caatgccgac gaagaagctt
     1921 atgacacgct ggtggtgaag gaattcgata ttacggaata ctgcactacg gcggcgcgta
     1981 tctgtgagga cctgatttcc cggctctgaa tcacggcgtg ttttgcggcg gcccgtccgc
     2041 ggcaaaacgc gcctttttca aatccaggta aaaggtttgt atgcggtaac attttcttgt
     2101 agataatccg gcttttttct cgtatcataa gcacaggggc aaaacgcccc ggctgcacgc
     2161 ccgctgtggg ctgtgcgcaa acgacaaaga ggtgattccg atgcagtgga aagaagagaa
     2221 ccgcgcgctg ttcgccgcct gcctgaacgg gctggaggac gagccaatgg tgcgcgcgat
     2281 ggacgccatt ccccagcatg tcaaaggcgt cagctgctat gaccattgcc tgttcgtagc
     2341 ttatgtgggt tttacgctgt gccgcatttt cgggctggat taccgcgccg ctgcccgtgc
     2401 agggctgttg cacgacctgt atctgcaaaa atgggaggag acggacgtcg ggcgcttccg
     2461 gcggctggtc atccatccgc agctcgcgct ggaaaatgcg cgcgccttcg gcctgtcccc
     2521 catggaagag gacatcatcg gcaagcacat gtggccgctg accgcgcctt tgccctatta
     2581 taaggagtcc tatatggtga gcctggcgga taagatctgc gccacattgg agatggccca
     2641 tctatatggc ctcttgggcg tgcgccggaa cctgcgtgcc tgccgccccg cgctgctgcg
     2701 ccgggccatg ctgtaaaacg aaccgaaata cagaagcccc gctgccggcc ggcagcgggg
     2761 cttctgttga ttagggattg tgaaaaagta cgtttttccc tgttccctgt tgattcgcaa
     2821 caaaaacaat ggaaaatact caaaaatcct ttattgtaaa atcatagatc ttgggtgaaa
     2881 ggagttttgt gcgggtgaaa gagaagcaga ggaaaaaatt ccattggtgg tatatccctc
     2941 tgggcatcgt gctggttctg gtgctgacgg cggggggcct tgtgctgtgg atgcacagca
     3001 tgctgccctg gccgaacatg gtggctatga ccagtaactt ccagatggag ctttcggacc
     3061 ccatgcgggc ctatgtcgtg ggcggcgagc gagattattc cacactgccc gaggtgctgg
     3121 cgctgcagga cggaacggga gtggaaacgc cggaggatat ggctttgcgc cgggcggagg
     3181 tgctggacct gtttgagaag catgtgtatg gtgtgctgcc gaagaatggc ttcgaaacgt
     3241 cttttgaggt tgtggaggaa ggcgaagcac tggacggcgc ggccttgcgc cgtcaggtga
     3301 agctcactgt cacaacggac aaaggctcct ccgatgcact gcttctgatg tatctgccca
     3361 attccgaccg gcccgcgccc gttgtggtgg ggctaaactt caacggcaac cacacggtgc
     3421 tggacgatcc ggccatcctg ccctcttatg ccagcgaaaa ggacgcggcc gtgctggaag
     3481 aggaacgcgg cggaaaagcg gagcgttggg ccattgaaga agctgtggca cgtggttacg
     3541 gcgtggccac tatttggtgc aacgattttg ccccggatga cgccaaggct tacagcagcc
     3601 gtataatttc gctgtttgac gagccggagt tcaaggccgt gggcgcgtgg gccttcggca
     3661 ttatgcgcgg cgtggattat cttgtgcagg acgcggatgt ggacgcggcg cgcattgcgg
     3721 atatcggtca ttcacgcctg ggcaaggcgg cggtttgggc aggagccaac gatgaacgta
     3781 tcgcgcttgt gatctccaac gattccggga acacgggcgc cagtctcacg cgggaaaacc
     3841 gcggcgagac actgaaatcc atcaatgctc tgtttcccca ctggttctgc acaaaatata
     3901 aggaatatgg agcggacgcc gcctccttgc ctgtggacca gcacttgctg ctggcctgca
     3961 tcgcaccgcg acgggtatat gtggcttgtg cggagggaga cctctgggcc gatccgcagg
     4021 gcgcatggaa cagcctgatg gccagccgcc ctgcctttgc gctgtacggg ctgaacgtac
     4081 tgccggacgg ctgcgtggcc gagggcgaaa cgcagcccca tgcgggccag gcttgctgga
     4141 gcggggccat gggctatcac aaccgcgccg gatgcatgac gtccagcgcg aggactggga
     4201 attttatttt cagtacatgg acgagtacct gaaggcgtga gagccttccg tctgcaaaaa
     4261 ttgaaaaaca gaactgccgc actggaattt ccgatgcggc agttgttttn nnnnnnnnna
     4321 aaatacagcc tgtggctgtt tgagcgctta cagaaataca ttcaattctc cggcatattc
     4381 attttcggca tgctgcaggc gctgagccag cgcgacagcg cccgggttcg cgtcgggcgc
     4441 gtccttgatg gcaatggtca tatctgtaac gcccacatgg ctgccttcaa tgagcatctc
     4501 cgcgattttg cggggagact tatcgtacat cgtctgcatc tttacgccca ttttggcatt
     4561 cagctttgta aaagcgttct ggccttcggc ctgctcgccc tctacggcca gcattgcatt
     4621 ggcccgtttg gaaaggtcct cgtatgtggc cagctggcgg ttcaggtgta ttttcaggcg
     4681 ttcgtcatcg gtgataccca aaagctgggt gacggtattt ttacccatct cggcattttt
     4741 tacgacctcg ttgagcaata gaacagtttg attatccatg gtgttccact ccttttagaa
     4801 ttagggtgcg ccatggccgg gaagaatatg cacttcaaaa aaggaggaat atttttgttg
     4861 atgctttaga attattaggg tatcttgttc aagtagaaga agggcgttat cctttgaata
     4921 tggatttaga ttccgaagca ttagttttta gtgcaaaaaa aggctagttt tattactagt
     4981 cttttttatg acaactttaa cgattcctta gttcaagata acgtttgtac cagatgttaa
     5041 cataagattg tgaaaatggc cccttaccat tgttaatcca atctaccaag attttaacat
     5101 gttcctttaa gatatagtca atatcaggag agtaatgcat ttctttttta tgtgtttcat
     5161 actcatctac atctagcaat tttttttcac catcagcgaa gactttgaca tctaggtcat
     5221 agtcaatata ttttagtgct tcttggtcca aaatatatgg actagctaaa ttgcagtagt
     5281 aggaaatgcc gttatcccta atcatggcta taatattaaa ccagtatttt ttgtggaaat
     5341 aaacaatagc aggttcacgt gttacccatc gtcttccgtc tgcttcagta acgagggtgt
     5401 ggtcgttaac gccaataaca gcattctcag ttgtctttag taccatagta tcgcgccaag
     5461 tacggtgcaa actaccatca tgcttataac tttgaattgt gataaagtcg ccttcttttg
     5521 gtaatttcat ggcgtcctct ttctacaata agggttatcc tctttattgt atcataattt
     5581 tgtgaaaaga gggggaatac ctataaatat tctcttagag cggaggctat agcat
//
LOCUS       DAWBSB010000146         5621 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_2633_length_5621_cov_2.702839, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000146 DAWBSB010000000
VERSION     DAWBSB010000146.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 5621)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5621)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5621
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2633_length_5621_cov_2.702839"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1753..1762
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1181 a         1417 c         1713 g         1300 t
ORIGIN      
        1 gtgccggtga tgctgccgga agtcaaatcc agaacacgtc cggcaggctt gtttgatatc
       61 gtttcatttg acatggcggt gcggatgcag agcctccgtt ctgttgactt tcagaatttt
      121 tcgcggcaga gaagcgccgc gcattccaca tgctttgtgc gcgggaacat atccactggc
      181 tgtacggcct gtacagcata tccgtgttga gaaagatatt taagatcccg cgcggcggtg
      241 gcaggattgc agctcaccat cacgatgcgc tggggggcca tggccaatac ggccttcagc
      301 gtcgactcgt cgcagccctt gcggggaggg tccagaacga cgatattggg cagcaggccg
      361 tcggcggcca gtttttgtgc ggcggttccg gcgtcggcac aaaaaaactc cgcgtgctct
      421 acccccattg cggcggcatt gcgccgggcg ctctccaccg cttcgggtac gatctctaca
      481 ccgataagcc tgccgcagtg ctccgccatg gaaaggccga tggtacccgc gccgcaataa
      541 aggtccagca gcgtatcctc ggggccgggc gcggcaaaat cagcagcaac accgtatagg
      601 cggttcgcgc cttcggtatt cacctgatag aaagaaagcg gaccgagcgc gacgggtacg
      661 ccgcgcatgg tatcgtggag cacgccggga ccgtacagcg tgatacattc ttttccggtg
      721 ataacattcg ttttgtgcgt gttgacgttc aataccacgc tctctacctc cggatgcgcc
      781 tttaccagcg cggcgcagat atcctgtgcg tgaggcaggc tgcgtccgtt gcagacaaga
      841 catacaagca cgcttccgtt tgtgacggcg tggcgcaaat aaatatggcg cgcgagccct
      901 cggtgtgcct gttcatcata gacggagata tgatattgcg tcagaagcgt gcacagcgtc
      961 gcggcgatcc ggttcagcgg tccgggctgg agcagacagt ccccgcaagg cagcggccga
     1021 tggctgcggc tcgcataaaa gccggcttga acactgccgt cccctgcgag aaacagagga
     1081 tactgcacct tgttccggta gcgctcggtc tgtggagagg gaagaatgtc ccgcacgggc
     1141 acatccagcc cgccgatgcg gcgcagggcg tccgccacga agccttgctt tgccgcaagc
     1201 tctgcttcgt aggaaaggtg ccggaaacag cagccgccgc accggccgaa aatagggcag
     1261 tccgcaggga tgcgcccggt gccgggcgtg aggacgcttt cgacgatgcc aaaggcgtaa
     1321 cttttgcaga tttttaccac cttgacggaa agcacgtcgc ccacggcggc aaacgggaca
     1381 aaaacggcct ggccctcaaa acgtccgacg ccgttgccgt cgctggacag tgcgtcgatg
     1441 gtaaggggga tgatctggtt tttgacaagc ggcatatgag cggttctcct cacaaaaaaa
     1501 gcggcaggca tgccgctttg aaaataacgg ggaagccgtg ctagcgcgcg gctttttcct
     1561 ggttgatgct ggtcaggtat ttgctgggcg gaacgccctt gctttttttg aacacgcggg
     1621 aaaaatagaa ctggtcaaaa aagccgacgg acacggcgat ctctgcaatg gaaagctgag
     1681 aattttttaa aaggaaacat gcctcgctga tgcggtagct ggtaaggtaa tcaatagggg
     1741 actgtcccac gtnnnnnnnn nngcggctga cgcccacggc cttggcgata tcgtcgatgc
     1801 tgatgtcatg ggaatagttg aactggatat acttgattgc gttgatgaca tactgagaac
     1861 tggaattgga ggcatgcggt tcaaggtcca ttgcctcctg catcagctca gcaatgaaaa
     1921 gatacagata tccgaccatc agcgtttcat tgcgcggctc gggcccccgg gacagaaaaa
     1981 tattgaacag cgcctttttt acgcgttcgg catttttgca gtggtgggtg ggcatgtcct
     2041 cccgaaaagg cagctgaagc accagcttgt tggcacaggc cccgttgaag cccacccagt
     2101 aatattccca cggttcctcc ttgtctgcgg aataagtgat gagctggctg ggctttgcaa
     2161 gaaagatatc ccccgcccga agctcataaa ctgtgttgtt taccgtatat gtgcctttcc
     2221 cgtcgacaac ataatggatc agatagtgat cccgtacgcc ggggccccag gtatagccgc
     2281 gctcacagct ctgcaggccg cagttgaaaa tggaaagttc cacattgtcg gtataattct
     2341 gcttgaacga ctgcttgtat gtgctgggca tccgattcac ctccggcgaa accgtgtggg
     2401 acaaaaaacg attccgcctc ttattcctca tatgatattt aattatagca aatcaatcaa
     2461 caaaagtcca ttttttcgtg atataaaata gtgtttacat caagcgcatg tctgttataa
     2521 tggatttgac accggggcag taccgggctg catttggagc ggcgctgaaa atgcgccgcc
     2581 atgcggcacc ccggcggaga agaaggcatc ggggccgctt ttcggcggcg tctataggat
     2641 aaacgaaaga caaggggagg ccggtaatat gtcaacggca aaagagttaa agaaagagat
     2701 cgagcgcggt gtatgggacg cgtccctggg aaggctgtat ggccggcggg caggcgtgct
     2761 ggagcgccag cgtgcgcggt acgcggatgc gctggactgt tttgcggcgc tatacggcgg
     2821 ggaacgggaa gccgccatct attccgcgcc cggccgcacc gagatcggag gtaaccacac
     2881 cgaccacaac cacggcgtgg taatggccgg cgcggtggat ctggacgtga ttgccgtggt
     2941 ttccaaaaac acggacgatg tggtacgtgt gaaatcgaaa ggctttgacg gcagggacga
     3001 agtgaagctg gagcagctgt ctcctgtgct ggcagagcag ggccgctccg ccgctctcat
     3061 ccgcggcgtg gctgcgggca tcgcaaagca gggcggtgta ctgggcggct tcgacgccta
     3121 taccacgtcc gatgtgctgc gcggctcagg gctgtccagt tcggctgcgt tcgaggttgt
     3181 cattggtact gtattcaatg gggaatacaa ccaaggccgt ttctccgcgg tagacatcgc
     3241 aaagatcagt caatatgcgg aaaatgaata ttttggcaaa cccagcggcc tgatggatca
     3301 gacagcctgc agcgtgggta gcgtgatcac cattgatttt cgtgatcctg ccagccccgt
     3361 agtggaaaaa gtggattttg atctggcaaa acacgattat tgtctgtgca ttacagatac
     3421 aaaagggagc catgcgtcgc ttaccgatga atatgcggcc gtccgccagg agatggaagc
     3481 agtggcggcc tatttcgata agccggtgct gcgcgaagtg gatgagtctg ctttttatgc
     3541 ggatattgct ggtgtgcgtg aaaaactggg ggaccgtgcc gtactgcgcg ctatccactt
     3601 ttttgcggac agtcggcgtg ccggtgcgtt atgcagtgcc atccgtgagg atcgctttga
     3661 agactttttg gaattgatcg tggcaggcgg ccattcgtcg tttgaataca accagaatgc
     3721 atacagtgca aaaaatccgc gggagcaggg cgtttcgctt ggtttggcac tttctcagca
     3781 tatactcgag gggtatggtg cgtggcgttt gcagggcggc ggctttgcgg gcaccattca
     3841 ggcgttcgtc ccggtgcatc tgctggaggc gtaccgcgcg gcgatggaca gcgtgttcgg
     3901 cgagggctcc tgttacgtgc tgaatgtgcg caactacggt gcgctgtgtg tgacagaggc
     3961 actgtaagcc gtgcgggaaa aatggtcggc cgccccgcgg cgaatcgcgg cgttcgatgc
     4021 cttgcgcggc tttgcactgg taaatatggc cgtataccat ggcgtttacg actgggtcta
     4081 tgtattcggc cgacctgcgg catggtttac gcaaacgggc aacgcgcata tctggcagca
     4141 attcatctgt tggacgttca ttctgctggc gggggctgtg tttccttatg ggaagcatgc
     4201 ggtacgccgt ggcgcggtca catttggctg cggatttctg ctcacggccg ttacgatgct
     4261 ggtgatgccg tcggagcaaa tactgttcgg cgttctgcac ttgattgggg cggccgtgct
     4321 gctcagcgcg ctgctgcgcc gctttctgga ccgcgcgcca gcgctgcccg gcgccatagc
     4381 ttcctttgta ttgtttttgt tcctgcgaag cgtcccgatg ggatatctgg gcattggcga
     4441 gctgcagctg tttgagctgc cgcgccagct gtacagcgtg ccgtttttgt ttccgcttgg
     4501 cctgcccggc ccgggcttct attcggcgga ttattttccg ctgatcccgt ggatatttct
     4561 gttctggacg gggcttttcc tgtggcgttt tctgcgcccg tatgccgaag agacggtgcg
     4621 cggctggcct gcctgccgtc cattggtttg gctggggcgg cacagcttgt gggcatattt
     4681 gctgcatcag cctgttttgt tgggctttat atttttgtta tcgatattga caggcagttg
     4741 ataagaagaa aggatgtaga aaatggtttt ggtctggtat ccgaaatgtt ccacctgcca
     4801 gcgtgccaag gcatggctgg atgagcatgg cgtgacctat acgctgcgcg acatcaagga
     4861 agaaaacccg aacgcggagg agctgcgggc ttggcatgaa aaaagcggcc tgcctctccg
     4921 gcgttttttc aacacaagcg gtctgctgta caaaggcatg gcgctgaagg acaagctgcc
     4981 tggaatgacg gacaatgagc agtacatttt gttgggtgaa tacggaatgc ttgtgaagcg
     5041 gcctttgatt gtagcggacg attttgtgct ggtgggtttc cgcgaggcgg aatggtctgc
     5101 agcgctcggc tgataggaga tatacctgcg ggtgtgcagt tcggcttcga acgcaaacaa
     5161 gcccttgtga aaatgttccg tacaatggtc ataagaccaa agcgcgtctc tggaaagcat
     5221 gggctgtgct tttgcgcaaa aagaaaaccc acaaaacagt ctgcaggctg ttttatgggt
     5281 tttcttactg gagctggtga tccgattcga acggacgacc tgctcattac gagtgagctg
     5341 ctctaccgac tgagccacac cagcatatgg cgcttaaatc aagtgccttt atctattata
     5401 aggaattttt tgcgttcgtc aagctttttc tgaaaagttg tggacgaaaa ttttattttg
     5461 cggtacaata agcaaacaga gaagtttcgg gagggaaaag aatggttaca gaatgcgttg
     5521 cgatactggc aatctcactt tgcctcgttg tggtattcat ccgtgcgggg cacggggatt
     5581 acgcagcgag tgtcacgccc atcctggccg ttcccttcgt g
//
LOCUS       DAWBSB010000147         5590 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_2648_length_5590_cov_2.534417, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000147 DAWBSB010000000
VERSION     DAWBSB010000147.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 5590)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5590)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5590
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2648_length_5590_cov_2.534417"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3233..3242
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3594..3603
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1031 a         1747 c         1619 g         1173 t
ORIGIN      
        1 tgtgcgccag cacaggggcg tgtgccttgc acacgcgcag ccgcgcaagc ggcgggcttg
       61 aggttattat accacatccc ccggcaggct ttatacgttc tttttcaaat cacctgacaa
      121 acttcgtctc aacatgctac aataaggcca tggcaaccgc cggttgccga aatggaacac
      181 ccagttggtg gcctcgcgcc cgcgcttttg ttaggatgaa atcggagggc gggcctgttc
      241 ccgcctcact tttcctcagg aggacacaac tatgacgatc aacgaacgca ttcttgcgct
      301 gcgccgggcg gcaggccttt cccaggaggc tttggccgaa caggtgggcg tctcccgtca
      361 ggccatcggc aaatgggaga gcggcgcttc tctgcccggg ctggataatc tgcaggagct
      421 ggcccgcgcg ctgggcgtaa gctgcgacga actgctgaca ggaaaagagt ccggaacgga
      481 aaataccgcc ggacaggacg gtgtggcgct ggaaagcgta aaagccctgc tggacgcgca
      541 aaatgcggcg caggcccatg cggcccaccg ccagcgcatc cttcttgcgg gccttgccgc
      601 cgcgctcgcc tgcgtgggtg cggcgctggt gtacctcggt gtgtattacg ggggccgcat
      661 gcgggagctt tccagccgtg tggacgacat cggcgcaaac gtggccggca tcgaaggcag
      721 catcgatatg cgtatctcat ccatccagtc cagcatagaa gagagcctgc gccagcaggc
      781 cagcatcgtg gctggttttg attgggccta cggcgcgccc ggcacagaac gccgcgtacc
      841 gttgacggtt acggccaccc ccaaaaacta ccgcgacggt ctttccgccg tgttcactct
      901 tacgccggag agcggtgtgc ctcagacagc cgccgggacg ctgctggcgg gcaatgcgtt
      961 tcgtgcggag ttggccgttc ccctggaaat ggaataccag gacttttcgg tatccgtcag
     1021 cttcacgctg gatggggagg cccaaacgga ggaactgttc cgggaatacg attttgtctc
     1081 gggctaccag acgacggttt cggtgacgcc cacagacttt aaagcacagc actccgggcg
     1141 caggacggac ggtaccgccg gaatgacgct gggcggggat tttgaacttt cggtaaatcg
     1201 cgcttaccgc gagaactccg ccgttcccgc gcatgcggta atcgagctgg tgatcgacgg
     1261 cgaggcggta cagcggcagg agatggatct ttcggctgat tttcagcttg tctccggcgg
     1321 cgccggaacc gccgatgaac aggcggaaga tatcctttcc ggcatcacct actatctttc
     1381 cttcccggaa gaggtctacg ccgcaccgca gagggaggcg gagcttgtgg ccgccgtcac
     1441 cgacagcagc gggcttaccg tcaccgcacg gcgggacctg tatcgtgttg gttgaatcct
     1501 atactccaaa cgccgtgcgg cggcattctg cccgttgcac ggcgttttct gtctccggcc
     1561 gcatttgttt gcctgtgcgt ccgtgctttg acaaaattta ctttttgtct cttttcttct
     1621 ttgtgcgcag cgggtatcat aatagcatac gttggggcgg cacgtctttt cacctccggt
     1681 atggcggagc acgctccatc cacaacagga gcgatgaagt atgagcgttt attcttacgg
     1741 cccgcagggg ccggacggac aggggtatca ttcgcaggga aaccggcgtg cggacgagcc
     1801 gttcgctgcg ccgggcggca gcgggcccgt gatcgacatg gtccccgcaa ggcagagaaa
     1861 gcgccacccg tttttgtacg ggcttgtgct ggtggctctg tgcggcgcag cggcctattt
     1921 cggcactgta tacgccaaca gccgtaccat gcccgaaccg aagaacgtcg tggtgtatca
     1981 ggcgccgtcc ggaaacgcgc ccgtgaccgg gggcgcgcgc ctggatgcgg ccagtgttgc
     2041 cgcggcggcc agcgattccg tggttgagat cacaacggaa tccgtgcaga ccggcagcta
     2101 tttcaaccaa tatgttgcca caggcgcagg cagcggtgtg atcatctctg ccgacggcta
     2161 tatcgtcaca aacgaccacg ttatcagcgg tgcaaacagc attgcggtgc gcctgcatga
     2221 cggcaccacc ctggatgccg ctttggtggc caccgacgcc aagaccgacc tcgctgttgt
     2281 ccgcgtacag gcacaggggc tcattcccgc cacactgggc acctcggcag accttgtggt
     2341 gggccagccc gtggttgcca tcggtaaccc gctgggccgt ttgggcggca cggtaacgga
     2401 cggtatcatc agcgccaagg accgggtgat cagcattgac ggagagcata tgacgctgct
     2461 gcagaccaac gcagccgtca accccggcaa ctccggcggc ggactgttcg acgcgcaggc
     2521 gcgtctggtg ggcgtggtga actccaaatc ctccggctcg gacgtggagg gccttggctt
     2581 cgccattccc atcgacacag cgcgtcccgt catcgagcag ctcatcaccc agggctatgt
     2641 cagcggcagg ccgtcgctgg gcatggagct ggtggagctg acatcctacc gctctgcgat
     2701 tttcgcaggc accggacggc tgggcgtata tgtgaacaac ggcggcaaaa cgggcctgac
     2761 gaacggcgat taccttctct ccatcgacgg gcttgccatt gaaacgatga ccgacgtaaa
     2821 gaccgctctg tccgggcact cgcccgggga cacggtgacg gtgcagtacc tgcacagcgg
     2881 tgcggtacgg gaagccgccg tggtacttgt ggaggatatt cccgctgcat gagctttttg
     2941 cgcacttttt gaaaaaaacg taaacttttg cggatatcct atattttccg cacggacagg
     3001 ggtataataa agcattatgg gtgctgtata tttttcacgg tgcccaatca ccggcacgcg
     3061 ctgcgcagcc ggacatggag gtgggcgctt gcaaatcgaa aagatgcggg cctttatcat
     3121 cagggtcctg ttttactgca tcatcctcgg gctggcctac gccgtgctga aatatgcgct
     3181 gccgttcctg atgccctttc tggtagcgtt tcttattgcc tttatcctga aannnnnnnn
     3241 nngcccgttt cggttttgct gctcattgcg ttttatgttc tggcggccgc tctcattacg
     3301 gtgctgggta cgcggcttgt gattttctgc cgcgatattt tctacgcgct gccgcaggtg
     3361 tacgacacgg ttgtggcccc ggcgctgaat tcgctgcagg acacactgga aaactgggtg
     3421 atggccctga accccacgct gacggatttt gtggaaagcg ccggcaacag cctgtcgtcc
     3481 tcgctgtcct caatggtcac cgccatctcc acaggcgctc agagcgcggt aacgaatgcg
     3541 gccggttccg tgccgtcgtt cctcgttaag ttcatcatca ccatcgtcgc gtcnnnnnnn
     3601 nnntcggatt attatgccat cacctctttc ctggcgcgtc agctgcccca aaaagcacgg
     3661 gacatgctgt tcaaggtgaa agaaaagggc gtggacgtta ttttcaaatt cggacgggcc
     3721 tacgccattt tgctgagcat cacctttgtg gagcttctca tcggtttttc tctgttgcgg
     3781 gtggactacg ccctgctcat cgcgctgctc accgccattg tggacatcct gcctgtgctc
     3841 ggcaccggca cggtgctcat cccgtgggcg gcggccatgc tgattctggg caacttcccg
     3901 ctgggcatcg ggcttcttat tttatatgcc atcatcaccg tagtgcgcca gatgctggag
     3961 ccgcgcgtgg taggcaagca gatcggcctg tatccgctgg taacgcttgt gtgcatgttt
     4021 gtcggcacct atctgttcgg ttttgtaggg ctgttcggcc tgcccatcat cgccacggtc
     4081 ctggtgcagc tcaaccaaag cggacacatc cacctgttca agtgagcctg tgcagagcat
     4141 gaaaaaagcg cgtttgacat tttgtcaaac gcgctttttt atttttcccg gtatggctca
     4201 gcgctctgcg ggccgcacgg gcagcagctc aatatagccc cggtcaccca caggctccaa
     4261 ttgtatgcgt gggttcgcgc cgtcccacac atagcccaga caggcggggt cgtacttctc
     4321 gatggcgccg cgcacctgcc cgatggcctc gcaataatcc tcctccgcaa acggctcgcc
     4381 ttgaaacatc aaatattccg ccgccggcag gcggatcaca tcaaagccct cgggcacggg
     4441 accgtcataa tcctctgcgg tctccacacc ctgtacatat tcggacgtac ccggagcgcg
     4501 ctgctccggc ggcagccaca ggcacaccgg ctctccgcaa agggacttca tactggtgag
     4561 cgtgccccaa acatcgcagc cgacctcttc acagtaggcg aaataatccc gcgcctttac
     4621 tccgcgcttc aaaatgacct tccgcgcggg cttttccaca cgctgtacaa acaccgtttt
     4681 cacattttcc atttttttcg gctcctttcg caattcccgg tatcttaccc cgtagggaac
     4741 aaaaaggcac agcgggacag gctttgccgc gtattcgccc gggttgcagc cgaacgcgcg
     4801 aaaaaacgcc cgctgataac cgtccacgct gccgaacccc agctcgaacg ccacgtctgt
     4861 aatgcggcag ccttcgtccc gcaggcgcaa tgcggagcgg gacagacgca gccgacggat
     4921 ataatccgcc ggcgtcagcc ctgtgtgccg ccggaacagc cggtacgcat accaaggcga
     4981 aaacagcgcg gcccgggcca ggtccgccgg agtgatctcc ccagccagat tctgttcgat
     5041 ataatcctgc atgcgctgta ccgcttcgat ctgctgcgtc atgccgttcc ctcccgacac
     5101 cgaggcttcc gtcctgccgc cagacgcttg cttcgcctta agtgcacctt cgcccgcggc
     5161 ccgcatctgc caggcaggca taggatacca caccgcctcc ggcccgcgct cgaccttttt
     5221 tgccttttcg gcctcagcgc gtcgccgccg cgcatacgcc ccggcattcc gccgcgcctg
     5281 ccgcgcgcag agcttttgca cattcgttca gcgtggagcc tgtggtgagc acatcatcca
     5341 ccagcaaaat ccgctttccc tccagcagcg catccggccg ggctgaaaat gcattcttca
     5401 aatttttccg gcgttccccg ccggaaagag ccatttgccg gggcgtttcc cgtattttgc
     5461 aaagcgcctg catcacaggc agccccagct cccggcccag cgcgcgcgcc agcatcaacg
     5521 gcacgtcata gccgcggctc cgccgttccc ctctgccgga aggcaccggg accaatacat
     5581 ccacatcccc
//
LOCUS       DAWBSB010000148         5553 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_2673_length_5553_cov_3.257548, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000148 DAWBSB010000000
VERSION     DAWBSB010000148.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 5553)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5553)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5553
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2673_length_5553_cov_3.257548"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1238 a         1532 c         1821 g          962 t
ORIGIN      
        1 ggatgcggcc taaaaaaagg ccgatctgtc cggcgtgctg acggcggaca tgctgggcaa
       61 catcctgatg gcccagaact tcaacatgcc cgcgggctat atcatcgagg gtgaggagca
      121 gtatcttgtc aaggtgggtg aggagtacgc ttctctggag gagctggaaa acacgctgct
      181 gatgcacatg gatgtggacg gcgtgggcga tgtgcgcctg tccgatgtgg cggatatcgc
      241 tctgacggac aacgcgggcg aaagctatgc caaggtgaac ggcaacgacg gcgtggtgct
      301 gtcgttccag aagcagagca cggcgtccac ggccacggtg agccagcgca tcaacagcgc
      361 catcgcacag ctgcaggagc agaacccggg catgcacatc acgccgctga tggatcaggg
      421 cgactacatc gacatggtgg tgggcagcgt cctttccaat ctgctgtggg gcggcctgct
      481 ggccatcatc gtgctgatct ttttcctcaa ggacgcaaag cccacattta ttgtggcgtg
      541 cagcatcccg ttcagcctga tgtgtgccat cacgctgatg tatttcacag atgtgacgct
      601 gaacatcatc tcgctgtcgg gccttgcgct gggcgtgggc atgctggtgg acaacagcat
      661 tgttgtcatc gaaaatattt atcgtctgcg ttctctgggc gtgcctgcct ccaaggcggc
      721 catccagggg gcgaagcagg tgtccggcgc tatttttgcc tccacgctca ccaccatatg
      781 tgtgttcctg cccattgtgt tcacgcaggg catttcccgc gagctgttca cggatatggg
      841 cctgaccatt gcctattcgc tgctggccag cctggtggta gccctgacgc tggtgcccgc
      901 catgggtgcc gccgtgctga aaaacaccaa agagaaaagc cacaggtggt tcgacgcctt
      961 tgtggaggga taccagaggc tgctgggttg ggcgctgcgg cacaaagcgc cggtgctggc
     1021 gggcgtgacg gcgctgctgg ccatcagcat tttcctgaca acgcagatgg gcacagcctt
     1081 catcccggcg atggacagcc cgcagatgtc ggccacactg acgatgccca ggggcgcggc
     1141 gcagcaggat gcctatgcca tggcggatac cgtcatggag cgcatcgcgg ccgtggatgg
     1201 tgtggagacc gtaggcgcga tgtccggcgg cagcggtatg tcctcgatga tgggcggctc
     1261 gtcctccggc ggcagcatca cttattatat cctgctgtct gacgaccgca ccgccaccaa
     1321 tgccgacgtt tccgctgcca ttgaagcgca gctggcggac ctggactgca ccgtggaggt
     1381 gcaggaatcc acgatggata tgtccgctct gggcggcagc ggtgtggagc ttgtcatcac
     1441 cgggcgcggc ctggacgaaa tgaacgccat cgcggatgac ctgcgcggta ttctgcgcag
     1501 tacggaaggc cttgtggata tctcggaaaa ttcggtcacg ggaaacccgg agacacgcat
     1561 cacagtggac aaatacaaag cgatgcagca cgggctgacc gtggcgcaga tatacagtga
     1621 gcttgccgcc gagctgaagg ccgagaacac ggccaccacg ctgacgctgg acggcacgga
     1681 tacgcccgtg gtcgtggtga agccggcagg acaggcgccc acgcggggca acatcatgga
     1741 tcacgcgttc acggtgtcga acgcggaggg cgaggaggag accgtccgtc tgtacgacat
     1801 cgcggcaaag caggagaccg acagcgtttc ctccattaac cgggaaaacg gcgcgcgcac
     1861 catgagcgtg agcgcgggcg tggacgcccg gcacaacatc ggcctcgtga gccgcgagct
     1921 ggaaaaaaag cttgcggatt acgagctgcc cgaaggctat accgttgaga tcgcgggcga
     1981 aaatgaaacc atcaacagcg ccatgacgga ccttgtcaaa atgatcgcgc tggccgttgt
     2041 gttcatttat ctcatcatgg tggctcaatt ccaaagcctg atgagcccgt ttatcgtgat
     2101 gttcacgatc ccgctggcat ttacgggcgg cctgcttgcg ctgtggctca caggcagcga
     2161 gctttccgtc attgccatgc tgggcttcct tgttctggcg ggcgtggtgg taaacaacgg
     2221 aatcgtgttc gtagacaaca tcaaccagct gcggctggcg ggcatggacc gcacggaggc
     2281 cattctggaa acgggccgca cgcgcatccg cccggtgctg atgaccgcgc tcaccaccat
     2341 tttggccatg tccacgatgg cgcttggcat tggcgacggc gcggagatga cacagcccat
     2401 ggccatcgtt accatcggcg gcctcacgta cgccacgctg ctgacgctgc tggtcgtgcc
     2461 cgtactgtat gatatcttcc gcaaaaagcc gctgtatgac ccggaactgg acgatgcggc
     2521 ggcagacgcg gcgaaggaac tgccgcagcc cgcggcaggc tgaggaggga ttcgttatgg
     2581 cgaaaaacgc ggaaaaggag accgctctgt acgaggcggt attccggctg ctggcccggg
     2641 gcgaaaaaat gtacagcgta acggtgcagc agatcgcgga ggaggccggc atcggaaagg
     2701 gaacggtata tgaatatttc acctcgcggg aggaaatcat atcaaaagcg atggcctacc
     2761 gccttcgcgg ggaggcggac gcgctctctg agcgggtaga caaagcggaa ggctttgagc
     2821 agaaactgga cacactgctg tgctttgcgc gggattccgt acaggcgcag gcctccggca
     2881 tgaaggtgct gttcgccagt gcgcgcacgc tggaatctcc cgaggccgtg tgccgccaca
     2941 ttggagatgc acaggtggtg gagcagcttc gcacaatgat cgcgcgcatt gtggagagcg
     3001 gtgtgcggga agggctgctg cgccaaccgc ccagtgaagc gtatgcgctg atggcgcttg
     3061 cgggcgcggt gttcgggtat gccaatttgc agcatattct gccgggccag gatgacgccg
     3121 cgctgcggga ggacgccaga caaatggtgc gccgtgcgct gtcatagcga aaagcatggt
     3181 gcaagatcct gcggaaaaca aacaatttag ccaaagaaac aaacagatac ctgtgcagct
     3241 tgtacaatgc tccgaaatca ggacgggcag attgaatttt ttgacttatt atggtataat
     3301 ttcccaaaag ggggttgctt tgagatgaac gagaaaaaac ctgtatcgaa tgtttgttat
     3361 caaatcgcgg cgaaaaacgg ccgcgtgctt gaagttgccg atttcaacac tgccagcggc
     3421 gctgccgtgc agctctggga caatgtgaag gaagacagcc agatctggct gcttgtggaa
     3481 gtggccgagg gcctttacaa gctggagaat aagctgacgg gcaaggtgct cgacgtgatc
     3541 aacgccggtt cccagaacgg cgcatggctg caccagtggg attatatcgg caaggacaac
     3601 cagctctgga cgctggagcc ggcggcggac ggccgctgga aggtgcgctc caagctgtct
     3661 ggccgggtgc tggatatcgt gggcatgagc caggaaaacg gcgcgcgcct gcagatatgg
     3721 gatgatgtgg atggcgaaaa ccaggcctgg aaatttacgg aagtaaaagc ggcgaaaaag
     3781 ccggcggcca aaaaggctgc cgcgaagaga gcggcggcgg aaaaagacgg gcctgccaag
     3841 gcggaaaaga cgcctgcaaa acgcggccgt aagcccgctg tgaaaaaggc ggaggaggca
     3901 aaagccgggg cgcccgcaaa ggcgtccgaa gcgcccaaag cggcggcgcc tgcaaagaaa
     3961 gcagcgccgg aaaaaaagcc ggaaccggca aagaaagcag aaccggcgaa aaaagccgag
     4021 ccggttcctg cgcctgagaa ggggaaaccc acggccggga agtctgcgcc gaagaaaaaa
     4081 taaaagacgg cttaggcacg ttcgggagcc gaagcatgga aaaccaaaaa tgccggaaag
     4141 cgctgaaaaa tgcgcaaccg ggagaaaaca cggccccggc tgcggcagtt gcccgcagcc
     4201 ggggcttttt tgcgcaaaac agcccttgac ggagcgcggc gggggaggta tgctaagaac
     4261 cgtgcctgcg gcgcgtcttg ctgcggcggg ctggaaagga tggaacaaaa tggcaaaaca
     4321 gatcactgca attatcgtgg gcgcgggaca ccgcgcactg ctttacagta cctatgcgct
     4381 ggaaaatcca caggcgctca aaatcgtcgg cgtggccgac ccggacccca tccgccgcag
     4441 aaaaacggcg gagatgcacg ggtttggaga ggatatgtgc tttgaaagcg ccgaggcgct
     4501 ggcggagcgc cccaggctgg ccgatgccgt catcaacggc accatggata cccagcatgt
     4561 aaaaaccgcc gtcccgctgc tgcgccgggg ctacgacatg cttctggaaa agcccttcgc
     4621 cgtcaatgag gacgagatgt gggagctgca gcgcgtcgcc ggcgagaccg gccgcagggt
     4681 gatgatctgc cacgtactgc gctacgcgcc gttctatgtg gccgtgaagg agcgcctgct
     4741 ggccggggag atcggcgata tcatcaacat ccaggccacc gagcacgtca gctatcatca
     4801 cctggcggtg tcttttgtgc gcggcaaatg gggcaacgag gaaaagtgcg gcgcgcccat
     4861 gctgctggcc aaatgctgcc acgacatgga tctcatcatg tggctgaaaa gcggcgtcgc
     4921 gccccggcag gtcgccagct tcggcagcga ctttcagttc gacccggcca agaagcccgc
     4981 aggcgcgggc aagcggtgcc tggtggactg cggcatcgag gaaaactgcc tgtactcggc
     5041 caaaaagcat tacatcgacc atccggaccg ctggagcttt tatgtgtggg actgcctgga
     5101 gcatctggaa aagcccacca ttgaggacaa gatccagtcg ctgaaaacgg ataacatcca
     5161 cggccgctgc gtgtgggact gcgagcacac agtggtggac caccagtcgg tggccatcaa
     5221 tttcgcggac ggcgcaacag ccacgctgaa catgatcggc ggcacggcca aggcggagcg
     5281 caacctgcac atcatcggca caaagggcga gatcaaaggc gtgttcgacg attcggtgtt
     5341 tacggtgcgc accatcgaca cggccagcga gagcggctgg cgcgaggaag tggtggacct
     5401 tgccatcggc ggagacaaaa gcggcatgac aggctcccac ggcggcggag acctgcggct
     5461 ggtggaggac tttgtgcgcg tgctgcaggg cgaacagccg tccatctcct gcacgaacat
     5521 caacgattcc ctcaatggcc atctggctgt gtt
//
LOCUS       DAWBSB010000149         5527 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_2682_length_5527_cov_2.652778, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000149 DAWBSB010000000
VERSION     DAWBSB010000149.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 5527)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5527)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5527
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2682_length_5527_cov_2.652778"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3106..3115
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4657..4666
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5272..5281
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1358 a         1593 c         1421 g         1125 t
ORIGIN      
        1 gatatctccc cctgtttggg cttgagcagc cgggaaacag catggagcgt ggtcgttttg
       61 cccgcaccgt ttgcgccgat cagcgtgacg atctcgccct cgttcacatc gaatgaaatg
      121 cccttgatgg cgttgatggc gccgtaattt acaacaaggt ccttgaccga aagcatcacg
      181 cccattacgc ctcacctccc aaatatgccg caataacctt tgggtcgctg cggacggtct
      241 cggggtcgcc ctccgcgatg atacggccgt aatccaatac cgtgatatgc tcacacaggc
      301 ccataacaaa gctcatgtcg tgttcgatga gcagaaccgc gatgtcgaat ttatcccgca
      361 caaaacggac ggtctccatc agttcggcag tctcattggg gttcatgcca gccgcaggct
      421 cgtccagcag cagcagcttg ggattcgttg ccaacgctct ggcgatctcc agcttgcgct
      481 gctttccgta gggcaggctg gaggccggct gccccgcttc tccgtccaaa tcgaagatct
      541 tcagcagctc cattgccttt tcattcatgg cccgctcctt cacaaaatag gagggcagac
      601 gcagaattcc cgtggccgtg gtgtatttca cctggttatg caggcccacc ttcacgttgt
      661 ccagcaccgt catatccttg aacagacgga tattctgaaa cgtgcgcgcg atgcccgcct
      721 tattgatgac agccggcttg aggccaacga tgctttttcc gtccagcacg atatcgcctt
      781 ccgtgggctg gtacacaccg gtgagcatgt tgaacaccgt tgttttgccc gcaccgttgg
      841 gaccgataag gccgtacaga tggccctttt caatggaaag cgcaaatttt tccacggcct
      901 gcaggccgcc gaatgcgatg ccaaggttcg ttacttccag caaagccatg catcacacct
      961 ccttctcgcc cgccgcagcc ttcttgcgcg gcagcggcag cctggcggcc agcctgcggc
     1021 gccaggccac aagctgcgga ctctggttga acagcatcat cacaatgagc acgacagcat
     1081 acaccagcat gcggtaatcg ctgaagccgc gcagcagctc cggcaggatg gtgagcagcg
     1141 cagccgcaat gatggaacca cggatgttgc ccataccgcc cacaacgaca aacaccagaa
     1201 tcagaatgga agtgttgtaa tcaaactttt tcgccaccag cgaggagaaa ttatgcgcat
     1261 acagcgcgcc cgccaaaccg gccagcgctg cggaaatagt aaacgcgatg agcttatacc
     1321 gcgtgacatt gacgcccacg gattccgctg cgatgcggtt gtcccggatg gacataatcg
     1381 cacggcccgt gcgggaattg atgaggttca gaacgatgaa gagcgtgatg agaataagta
     1441 caaagcccag cccgaaggtg gcgcttcgcg gcatgccggt gatgccctgt gcgccgttga
     1501 tgatcacatc gcctgtctcg tccaggttca gcgaaagcgc gtccttcagc gaaacatgga
     1561 agccggacgc atctctgccg acatagaagg aattgagcag gtttttgatg atctcaccaa
     1621 aggccagcgt tacgatggca agatagtccc cctgcaggcg caatacagga atgccaatca
     1681 aaaaaccgaa caaggctgcg gccgcggtcc ccgccagcaa cgccagcgta aagcagagcc
     1741 agcccggcac ggcgtcccca acacacacgg agacaaacgc ccccacaaaa ccgcccacgc
     1801 tcataaagcc cgcatgcccc aggctcagct cgcccaggat gcccacggtg aggttgaggg
     1861 aaattgccat aatgatatat gcgcaaatcg gaacgagctg ccccgacagg gaatttgtga
     1921 gcatgcccat gcctgaaagc agctggatgg ccaaaaacat cacaacgacc attccatagg
     1981 tcacaagatc gcgccggaaa ctgcggctca aagttttcat tttcattgcg cccacctaca
     2041 ctttctctcg cgtctgcttg cccagtatgc ccgcagggcg gacaagcagt acgatgatga
     2101 gcacgccgaa cacgatggcg tccgccatct gggacgaaat atacgctttg ctcaggctct
     2161 cgatgatgcc cagcagcacg ccgcccagca tcgcgccggg gatggaaccg atgccgccga
     2221 acaccgcagc cacaaaagcc ttgatgccgg gcatcgcgcc cgtcgttggc atcagcgtgg
     2281 gataggccga acaaagcagc acgcccgcga cggccgccaa agcactgccg atggcaaaag
     2341 tgagcgaaat ggtggcgttc acattgatgc ccataagctg tgcggctcct ttgtcctcgg
     2401 atacggcctg catggcgtgg cccactttcg tcttgttgac aaacaacgtg agcgccacca
     2461 tgatgactgc ggatgtaaca atggtgacca gtgtctcgcc cgtaacgacg agttgcccgc
     2521 cgaagagacg gagcggttcg atggacacga ccgacgtgaa ggatttcggc gcagagcccc
     2581 agatgagcag cgccaggttc tgcagcagat agctcatgcc gatggccgta atgagcacgg
     2641 cgaggctggg cgcctcccgc agcggcttat aggccacgcg ttcgatggca acgcccagca
     2701 gcgtgcaaac caccaccgcc gcgcagattg ccgccagcgc gggcaggccc agcgacgtca
     2761 tcattacaaa agcaacgtag ccgcccacca tgatgacgtc gccatgcgcg aaattcagca
     2821 tacgggcaat gccgtacacc atcgtatagc ccagggcaat gaccgcataa atactgccca
     2881 ggctgatgcc gttcacaagg taagataaaa attgcatgtg tttcgcccct tttttgtttc
     2941 ccataaaacg ccgtgccgcc ggcgtccaag agaggatcgc tccacaggta ccagacttta
     3001 taagtatacc ataccgggtg ccgcccggca attgaaaacc acaaaaaacg gccttgcacg
     3061 ctgttttcca ccgaaaagat gtcgattgga aagaaggctg ccggtnnnnn nnnnnccttc
     3121 ttggcctttt tgtaacagaa attatgccgc ggtgtaaacg ccgtccttga tgatgtagac
     3181 aagcgggctc ttggcaggcg cgccgctggc gtcccaggta atgcccttgc cggtcaagcc
     3241 ttccagtgtg atctcagtaa aagcggcctt cagggcgtcg caaatggcgg acacatccat
     3301 gtcggctgta acgccggcct tctccacagc cgcgtggatg gcgtaaatgg cgtcataggc
     3361 atcggccgcg aactgattgg ggtcctcgcc gtaggcggcc gttttgtcat cggtagccga
     3421 agcggtgaac ggagtcatca gcatgacatc ctcggccaga ctcgtgtcga agttctccac
     3481 agacaccaga ccatccagac cgtcgcagcc aaagaacacc ggctcatagc cgatcttgtc
     3541 agcctgcgtc agaatcaggg acgcctcggt gtagtaaatg ggcaggaaca ccagctctgc
     3601 gcctgcagac tgggccttct gaatctggac tgtgaagtcc ttattgctgt cggcggtaaa
     3661 cgcctcgtca gaaacgatct ccaggccctt ttcctgcgcc tcggtcacga acgcatcgtg
     3721 aatgccggag gaatacgggt cagagctgtc atagatgacg gcgacctttg ccgcaaggct
     3781 gttctcaacg atataatcgg cggaaagcgt gccctgctcc gggtcggaga agcaaacacg
     3841 gaacgcattg tcgtttgcga tgcagccttc agcggagccg gacggcgtca gctggaacat
     3901 attgtcgttg gccgtctcgg ccgcaacggc gatgcaggga ttgctggtga cggtgcccat
     3961 caggatctgc atgccccagt ccttcaggct gttgtacgcg ttgatcgcct tctcggcatc
     4021 gttctcgtca tcctcgaatt tcatctcata ctgctggccg cccagcgcgt tgatctcggc
     4081 aatggcgatc tccgcgccgt tcttcacggc attgccgtaa accgcggtgc tgcctgtaac
     4141 agggccgata ccgccgatct taatggccgc gccggaaccc gccgtgccgc cggacggcgc
     4201 ggtggacgcc ggtgtgctgg agccggaacc gccgcacgcc gccagcgaaa aggcgaggca
     4261 gagggccagc gctgcgctca gaaacttttt catactttct tcctccaatg tttcaaactt
     4321 tccatacaca aagcttccaa caaagctttt gataggaatt gaatctattt taacgcggaa
     4381 gatgcacaaa attcaataca ctatttttac atacttttct ttgctaaagc gttcaattgt
     4441 gtgatattgc ataaaaaaac agaaaaaagc tccgtcgctc ccgtacatcc gggcaaagtt
     4501 gtccgccgta gacgtatatt cactctcttt tttgcacaag aatgcacacg gcaaacggaa
     4561 ccaagaaggc caatttcacc gtcacgcttt tccggtctcg gtttccggcg caaaacgcgg
     4621 ggccggttta cggccccgca catcgttctc gcgcatnnnn nnnnnnttac agcccccatt
     4681 tccatagtat ttttcgccct ttcgtacata caatacgcaa aatcttatcg tatggcttta
     4741 aattctcccc gcacacagga cagatcgata cattattcct aaccatatca cacctcacag
     4801 tgccggagtt gataattcct atactacaat atattctcac aaatattaat tcaaataacg
     4861 gaaaggagaa aacagtgctc ataaaatgcc tgagtgcgaa taacaagtaa gcgataaggc
     4921 tttgtcgtac ccacaccacg gataccctct ataaactaag cctcaacctc gaaagccaaa
     4981 aagaggtcct caaagaaaat gccccgatgc agacgtatgt attaaaagcc ggcgctataa
     5041 cagttcgagg caatcttata tacacccctg cgcacaaacc gcacgatggt ggaaagcact
     5101 ttgcagcaat ggcggagaac cacaaagtcg atgggtgcgc catcaagtac atgggtggtt
     5161 acgaaataaa cgatctcgct aaaagcgtat acactgaacg tgaaatttct tggctaaaaa
     5221 aggaacagag cttttgggct atctgcacat gcttttcaaa cggtttggaa tnnnnnnnnn
     5281 nacaatactt cgaattgaaa caattgtata atacaggcat gaaacacata acaagcccaa
     5341 tatgttatgt aaaacaaaac aattatggga ggaactgtcc atggaaacga tgacacccaa
     5401 agaaagaatt gacgcagtta ttaagcacca agctgtagac cgcactcctt ttaccgtagt
     5461 cgatggcgga gcatggattg ctaaaacaga gggcgtgaca tatcgggagc tatatagcag
     5521 gccggac
//
LOCUS       DAWBSB010000150         5373 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_2756_length_5373_cov_3.594396, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000150 DAWBSB010000000
VERSION     DAWBSB010000150.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 5373)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5373)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5373
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2756_length_5373_cov_3.594396"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1039 a         1542 c         1690 g         1102 t
ORIGIN      
        1 gcaaggaggc ctgcaaggcc gcgaaagctc atggcgtgaa gatcagctgc gacctgaact
       61 accgcggcaa gctgtggaca cgcgacgaag cccgcgccgc catgacggag ctgtgccagt
      121 atgtagacgt gtgcatctcc aacgaggagg atgccaaaga cgtattcggc atcgaggccg
      181 agaacaccga catcaccggc ggcgagctga acaaggaagg ctataagagc gtggccaaac
      241 agctggcgga caaattcaat ttcgagatgg tcgccatcac gctgcgcgag agcaaatctg
      301 cctttgacaa cgactggagc gccatgcttt acaacgtggc caaggatgag tactgcttct
      361 ccaagctgta tcatctgcac atcatcgacc gtatcggcgg cggcgacagc ttcggcggcg
      421 gcctgatcta cgccctgctg tccggcaagg acacccaggc cgcggtggag ttcgcggtgg
      481 cggcttccgc cctgaagcac tccatcgagg gcgactacaa cttcgtcacc gtgcccgaag
      541 tggaaaagct ggcgggcggc gacggttccg gacgtgtaca acggtaatta gcgcttaccg
      601 ttatacaata gatacgccgg tatttccggt gttcaaagag tcttcaagca ctcaaacact
      661 cattacctcc caaaagcaaa agggccgccc tgccgggcgg cccttttgca cgggagaggc
      721 aggaggagac ccgtgcaagc caaaaatccg aaaattcatg tagagcgctc gttttaccgc
      781 agcctgctgg cgctcacact gcctgtggcg ctgcagaatg tcatttcgta cagtgtcaac
      841 ctgatggata ccctgatgct gggcgggctg ggcgaggtgg cgctctctgc caccagtctg
      901 gccaaccagg tgttctttat ttataccgta gccatattcg gcgtggcggg cggcgccctc
      961 gtgctgtgca gccagtactg gggcaaaaag gacgtggatt ccatctgccg cgtcgccagc
     1021 ctggcgctgc gcatttcggc tgcggcgggg ctgctgttca cactggtgct gtttttgttc
     1081 ccgcgcccgg tgatgcagat attcaccgcc gagcccgccg ttatcgaggc cggcgcatcc
     1141 tatctgcgca tcgtgtcgct gtcgtatttc ttctacggcg tcacatccac attcctggtg
     1201 gtgctgcgca gcgtggagac cgtcaacgtc tcggtggcga tctacggcgt gtcgtttgcg
     1261 gtgaacgtgt ttttcaacta cgtgttcatc ttcggcaagt tcggcgcgcc gcgcatgggc
     1321 gtggcgggtg ccgccgtcgg cactgtgctg gcgcgcgtgg cggagttcgt gatgatcatc
     1381 gcctatatgc tcgtgtggga aaagaagatc cgcttccgcc tgcatatgct gctgtggccg
     1441 tcgggcggcc tcgcccggga cctggtgcgc tacggtttgc ccgtgatgct caacgagctg
     1501 ctgtggagcg tcgccatttc catgcactct gtcatcctgg ggcatatgag cagcgatatc
     1561 gtggcggcca acagcatttg caatgtgatg ttccagatgg caacgagcat catcctgggg
     1621 gtgtccaatg cctcggccgt tctggtgggc atgctggtgg gccgggggga ttatccgtac
     1681 gcccgggcct gtgtcaacaa gctcatccag atctacgccg tcatcggcgt ggtgtgcgcg
     1741 gcgttgctgc tggcggtgaa ggggccggtc atcggcattt atgacatcaa ggaaagcacc
     1801 cgcgtactga ccgaccaatt gatgtatgta tatgcggcgg ccattttctt tatggcgttc
     1861 acctgcccgc tgatcagcgg tgtgctgcgc ggcgcggggg atacccgttt cgcggctgtc
     1921 acggatgtgg gctgtatctg ggcgatggtc cccctgggcg cggcagcggc attctggctg
     1981 ggcgcgccgc cggtgctggt gctggccgtg ctgaaatgcg acatgccgct gaaggcattt
     2041 ttctgtctgc gccgcatcgc gggcggcaac tggatccgca acgtcacgcg gtaaaggccg
     2101 cgcgcggaaa atacggagag agggttgccc gcccgccaca tatccgggcg gacaaacgat
     2161 ttttcaacag gcaaagcggc cgtgtatttg cacggccgcg gttttgtgct atgatgaaaa
     2221 aaacgccccc cggcggcgcg ccggacgcca aggaggaaga aaaggagaaa cgccatgaat
     2281 ctgttcattt ccagcgatat cgaaggcacc tgcggcatcg ccgcatggag cgagaccgag
     2341 ccgggcaggc cggacgggga ttacgcctat tttaagcagc agatgagccg ggaggtggcc
     2401 gcggcctgcc gcggcgctct ggcggcaggc ggcgagcgca ttctggtaaa ggacgctcat
     2461 gattccgccc gcaacatcga cccctctgtt ctgcccgaag aggtgcagat caaccggggc
     2521 tgggcgggcg atgtttacag catgatgagc ggcatccagc acggcgattg ggacgcggca
     2581 gtctttaccg gttatcatgc ggccgcctgc tgcgacggca acccgctttc ccacaccatg
     2641 aacaccagtg tggattatgt ggagatcaac ggcgtgcgtg ccagcgaatt cacgctcaac
     2701 gcctatatgg cgggctatca cggcgtgccg gtgtgctttg tttccggtga tgcggcgctg
     2761 tgcgacagtg cacggcgcat gatacccgat atcgccacgg tgcccgtcaa taagggggac
     2821 ggaaactctg ccaccagcct gcatccggcg ctggccgtca agcgtatcga ggaaacgctg
     2881 cgcgcccagt tggagagcgg cgcgtaccgt acctgcatcg tgcccatgcc tccgaagttt
     2941 gagatcatca tccgctataa ggaacaccgg gcggcctacc gctgcagctt ctacccgggc
     3001 gtggaacagg tggacgaaaa aacgctgcgc ttcacctgtg tggattatat ggacgccatg
     3061 tgcctgtttc agttcgtgct gtaaggctcc ggccgcgcaa agcagaggag gaaagcggag
     3121 tatgacacaa aaggaacgca tgctggcggg cctgccctat aaggcgtgga tggacggcct
     3181 gccggaggaa cgcgcccggg cgcagcgtgc ctgctgggaa ttcaaccgtc tgccgccgga
     3241 tgcgcgtgca gagcgcacgg cgctgctgcg cggcctgttc ggcagaacgg gcgagcatct
     3301 gcacatcgag gagccgttcc actgcgacct cggctacaac attgaggtag gcgagtggtt
     3361 ctatgccaat tacaactgtg tgatcctgga cgtttgcagg gtgaccattg gcgacaatgt
     3421 gatgtttgcg cccaacgtcg ccatttatgc ggcgggccat ccggtacacc cggccgcgcg
     3481 caattcgggc tacgagtacg gccagcccgt cgccatcggg aataacgtct ggttgggcgg
     3541 caatacagtg gtcacgcccg gcgtgaccat cggggacaac gtggtggtgg gtgcgggcag
     3601 cgtcgtcacc cgggacatcc cggcaaatgt tgttgcggcg ggcaacccct gccgcgtgct
     3661 gcgcgccatc accgaagcgg acgcggatta ttattttaaa gaccggaaat tcgacgtgga
     3721 ttggcacgcc gagctttgat gcgcttcgtg cggcaataag gcccggagcc gtgcggaaag
     3781 cagcgcggct ccgggcctta ttgcgggatg cgccggaacg gcgcgcttag cgatgtttaa
     3841 aaaatagaga aaatgctgtg ttctgagaac ttttaacgaa gcaaacaccg attcctgtcg
     3901 gagaaaacag ctttttcgat tttttaagcg tcccctagtt gatggccttg ccgaagaagg
     3961 cgctggcggc cttgaccttg cccatcagcg cgtcgaactg tgcgggcgtc aggctttggg
     4021 cgccgtcgct tttggcgtgg ggcgggtcgt tgtggacttc gatcatcaat ccgtccgcgc
     4081 ccgcggcaat ggcggccatg gcaagcggct ccaccatcca ggcaatgccc gcggcgtggc
     4141 tggggtcaat gaccacgggc agatgggtct tgcttttgag catgggcacc gcggaaatgt
     4201 ccagtgtgtt gcgcatgctc gtttcaaacg tgcggatgcc gcgttcgcac agtacgacgt
     4261 tttcattgcc gccggccatg atatattcgg cgctcatcag aaattcttcg atggtgttgg
     4321 caaggccgcg tttgagcaga acgggcgttt gcagcttgcc cagttccttc agcaattcaa
     4381 aattctgcat gttgcgcgcg cccacttgga tcatatcaac gtcggcaaag aggggaacgt
     4441 gctcggtgtt catgatctcg gtgacgatgg gcagcccggt ggcggcgcgg gcctcgcgca
     4501 gaagctccag gccctcggcg cgcatgccct ggaacgcgta aggggaggtg cggggcttga
     4561 acgcgccgcc gcgcaggatg cccgcgccgc tgcgctgtac gtcgcgggcg acttccacga
     4621 tctggctctc gctctccacg ctgcacggcc cggccatcac ggagaaatgt ccgccgccga
     4681 tcgtatgtcc cgccacgtcc acagcggtgt ccgccggatg gaatttccgg ttggcctttt
     4741 tataaggctc gctgacccgc ttcacatcct cgacaaaatc attggcgcgc acaaggtcga
     4801 tgtccacgct ggatgtgtcg ccgatgaggc cgataaggtg cgtgtgctcg ccctcggaat
     4861 tgtgaatttt gaggccctgg gcttcaaaac cggcgcagag ctgcttcacc tgctccgggt
     4921 tcgcgttgtt tttaaaaata acgatcatac tgcatacccc tcttgcgatt taaaatttta
     4981 aaacaaaaaa gccggcagcg ctctgcgctg ccggcttatc agtttacaaa aaattatttt
     5041 gcatcctgaa ccgttccaca gcaaacagag ccattacctg taaagtaata gccaaaataa
     5101 taatagtttg cagttgtaaa cgaacggtcc ggcatggttt tcccccagcg gaaaagattt
     5161 gttagcagta tacgcgcatt cggcgcattt gtcaagggca gcctttcgcc ggggtaaacg
     5221 ggggccttgc gcttctcatc ccagagcctc gaaagccaga cgcaggccga gccccacgcc
     5281 ttgtgcgaag atggcggtcg cccggtgcag cccggcaaac tcctcgcggt cgcacagggc
     5341 ttcatacagc tcgttctggc tccgggaaaa ggt
//
LOCUS       DAWBSB010000151         5351 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_2766_length_5351_cov_2.931647, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000151 DAWBSB010000000
VERSION     DAWBSB010000151.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 5351)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5351)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5351
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2766_length_5351_cov_2.931647"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1916..1925
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1048 a         1754 c         1471 g         1068 t
ORIGIN      
        1 caccctattg atacgtacgg ggcactggaa aaatgcattc ccccgttgaa cagtcccgcc
       61 cggtgatatc cagccgcacc aggcaaagcc gtcctattga cacggcctct gccgcaccca
      121 ttaaaaccgc gtcacgccgt ccttgtctac acgggccagc accagtgcgg gcagctgggg
      181 acgccgggcc gcaaaggtga gcgcactgtg gaatttttct tccgcagccg caaatttcgc
      241 cgcatccccg gcgtacagcc gtgccagcac atcgcccttt ttcaccgggt cgcccgcttt
      301 tttcaacagg cggatgcccg ctgccgggtc aatgacgctc tcttttgttt cgcgccccgc
      361 gcccagcaga acggctgcaa tgccgatttt ctcggtgtcg gtggcataca gccagccgtc
      421 ctgcatgcac accacgtcat gttcatacgc ggctgcgggg aagtctgacg gatcgtctat
      481 cacggcaggg tcgccgccct gcgcggccac catggcccgc agctttgcca gcgccgcgcc
      541 gctgcgcagc gcgtcctccg ccatggcgcg gcatgccgca gcgccgcccc tgcctgccag
      601 ccacagcatg cccgacgcca gagacaggca cacctccgtc aggtccgcag ggcctttgcc
      661 ccgcagggtc tccaccgctt cgatgacctc caggctgttg ccgatattgc ggcccaacgg
      721 ggtatccata tctgtgatga gcgccgccgt gcgccgcccg ttatgctcac cgatgtccac
      781 catggccttc gccagcgcaa tggaatcctc cagcgttttc atgaacgcgc cgttccccgt
      841 ttttacatcc agcaggatgc agtccgcgcc ggaagccagc tttttactca tgatgctgct
      901 ggcgatgagc ggaatacagg aaaccgtagc cgttacatcc cgcagcgcat acatcttttt
      961 gtccgcagga gccaggttgc ccgtctgccc gatgaccgcg atgcctatgt cgttcacctg
     1021 ccggaaaaac gcttcctgcg tgagcgacac cgagcatccg gggatagcct cgatcttatc
     1081 cagcgtgccg cccgtatgcc ccaggccgcg gccgctcatc ttcgcaacct ttacgccgca
     1141 cgcggccacg atcggccccg ccaccagggt ggtcttgtcg cccacgcccc ccgtggaatg
     1201 tttgtcagcc ttgacgccgc cgatccccga aaggtccacc atatcgccgc tgtaagccat
     1261 ggctgccgta agctgggcgg tctccgttgc cgtcatgccg cgcaggtaaa cagccatcat
     1321 cagcgcactc atctggtaat ccgggatgct gcccgcggca aagccgttta ccgcgaacgc
     1381 gatctcctcc tgcgtcagcg taccgccgtc ccgctttttg gcgatgatat catacatgcg
     1441 cataaaaaaa tctcctttat ccgttttgtg tgcgcgccgg gcagccggcg cgcacgctgc
     1501 ttcagccgga acgcaaaggg gcggacggcc gcccggcgta ccgcccggcg gcggcagccc
     1561 gcccctgatt cattttcttt ccgcgccgct ttctaacggc tgcccttgtc gtagggaatg
     1621 ccttccgcac gcggcgccat ggaatttttg ctggtgaacg ccagcacgat cagtgtggct
     1681 acatagggca ccattttgta aacgtcgttg gggatgccca ggtttgccag gaacgggatg
     1741 cccgaatacg cggacgccac cgttttcatc aaaccgaaga agaacgccgc aaacaggatg
     1801 cggctgggtc tccattggcc gaagatgagc accgccagcg ccaaaaagcc gtagccggcc
     1861 actgtggcgt tgaagttggt ggatgttggc accacaaaca caaggccgcc cacgcnnnnn
     1921 nnnnncattg atgcccaccg aatctgccgc ctgcgggtgt tcgccgcacg cgcgcagccg
     1981 caggccaaag cgcgttttgt acaacaccac agccgatacc gccagcaccg caaagcccag
     2041 ataggtggtg atataggtat tctggaacag cagcggcccc agcacgggaa tgtcgcccag
     2101 caccggtacc ttggtgatgc ggaaggtgtt ggagaacggg atctgctgca cgctgcggat
     2161 catacgggcg gcaaaaatag cgaatgccgg agcgaacatg ttgatggccg tgccgctgat
     2221 gacctgatcc gccttcatat tgatggatgc atatgcatgc gccatggaga ccagcacgcc
     2281 caccgccgcc gcgataagca ccgccagcag gagcagcagc tggggcggca gggccgcgcc
     2341 gctgctctgc acgatgttga tgaacaggat gctggaaaac gcacccatga tcatgatgcc
     2401 ttccagcgcg atattgacga cgccgctgcg ctcactgaac atcccgccca acgccacgat
     2461 cagcagcgga atggagaaga acatcgtctg ctgtaccaga aaatatacgg tattcattca
     2521 gctccgcccc cttcctgcgg gcccctgccc gaaacagcgg gcctgccgtc cgccgcgcgc
     2581 ttttcttctc tgcgcttgac gcgccgctct acaaagccgc gcaaaagcag cgaaaaggcc
     2641 gcgaagtaga ttaccacgga aataatgata tcaatgacct gcggcgcaaa gccgtatacc
     2701 tgcatattga agccgccctg gttcaaatac gccacgaaca ggcctgagaa aatgatgccg
     2761 atgggattgt tgaggcccag cagcgccacc gggatgccgt tgaagccctc ggcggccagc
     2821 gtgtccagca cctcgattcc cttgcccgcg cccgccaggt acaggcatgc gccgcccagg
     2881 cccgagagcg cgcccgcaat gaccatggaa agcacgatgc tgcgtgtctc gttgatgccg
     2941 gcataccggg ccgcatcgcg gttgtagccg catgccttca gctcgtagcc gaattttgtt
     3001 ttttcaatga ggtaatagat aacgacgccc gcgaggacgg caatgaagat gcccgcgttg
     3061 gcgctggagg ccgtggctcc gctgcggaag atcaggtcca tgcccagctt gggcgcgttg
     3121 gcgttttccg ctaccggaag cgattggttc ttcagggaat cgaacaccgt cagctgaatg
     3181 aggaagttga cgccgtacat accgatataa ttcatcataa tgcaggcgat cacctcgttc
     3241 acgttgcaca cggccttcaa aaagcccgga acaaggcccc acaccgcgcc cgccagcatc
     3301 gcgcccagca ggcaggccag ccacaacagc gggccgggga tgaacgtgca tttcacaccc
     3361 atcagaacgg ccacatatgc gcccacgatg aactggccgg acgcgccgat gttgaacagg
     3421 cccgttttgt tggcaaagcc caccgaaagg cccgtcagaa tgatgggcgt ggctgcgtac
     3481 agcacctgcc ccaggttctt catgtcggaa aggccgcccg tcagaatggc cagcaggccc
     3541 cagaaggcat tggccgggtc tgccaccagc agcaccacaa agcccaccac aaggccgcac
     3601 aaaattgcca gaagcgaggc cataaagctg gaaaaacccg cttttgtata gaggtttcgc
     3661 cagcgcgccg atattttttt cattgcactg ccctcctccc ggaaccggcc atataaagcc
     3721 caagctcctg cacggtgact gttttcgggt caagatcggc cacgatccgg ccctcataga
     3781 taacgaggat gcggtctgaa agctgcatca cctcgtccag ctccaggctc acgagaagga
     3841 tggccttgcc cgcgtcccgc tcctccacca gcttgccgtg gatgtactcg atggcgccca
     3901 cgtccagccc gcgcgtgggc tggacggcga tgagcacgtc cgggtcgcgg tcgatctccc
     3961 gcgcgacgat ggccttctgc tggttgccgc cggacatgct gcgcgtgatg gtgcgcgcgc
     4021 cctggcccga gcggatatca aatttgccga tgagcttgtc tgcgtattcg tagatggcgt
     4081 cgtacttcaa aaagccgcgg ctgcagaact ccggctcaaa atagctttgc agcaccagat
     4141 tgtaggcaag gttataatcc agcacaaggc catggcggtg gcggtcctcg ggaatatgcc
     4201 ccagaccgcc cgcgttgcgc ctgcggatgg gcgcgtgggt aatatcctgt ccgttcagcg
     4261 tcacggtgcc ctcctccggc tccacaaggc ccgtcagcgc gtaaacaagc tccgtctgcc
     4321 cgtttccgtc gatgcccgcg atgcacacga tctcgccccg gcgcacgtca aaatccacat
     4381 ggtccaccac atttttgctg cctccgtgtc cctttacgca aaccccccgg gccgacagca
     4441 cgacctctcc cggctgcgcg ggcgtttttt ccacattcag gttcaccttg cggcccacca
     4501 tcatctcgct catctcctcc ttggtggtgg aggcgacgtc cacggtgccg atgtaccgcc
     4561 ccttgcgcag gacggtgcag cggtcggcca ccgctttgat ctcattgagc ttatgcgtga
     4621 tgaacaggat gctctttccc tcgctgacaa gctccttcat gatcttcatc agttcgtcga
     4681 tctcctgcgg cgtcagcacc gctgtgggct cgtcgaaaat aaggatctcg ttttcacggt
     4741 acagcatctt caggatctcc acacgctgct gcatgcccac cgtaatatcg gagatacagg
     4801 cgtccgggtc cacccgcagg ccgtaccgct ccgagagggc cgttaccttt tcccgggcct
     4861 tgtccatttt caaaaagccc cggctcgtgg tctccacgcc cagtatgatg ttttccagca
     4921 cagtgaagtt gtgcaccagc ttgaagtgct gatgcaccat gccgatgccc aaacggttcg
     4981 cgtcgtttgg gtccttgatc tggacctcct gcccgttttt ccggataacg cccttttccg
     5041 gctggtacat gccgaacagc acgctcatca gcgtggattt gcccgcgccg ttttccccca
     5101 gcagagcgtg gatctcgccc tttttcagct gcagcgttac gtcgtcgttt gcgatgatac
     5161 ccgggaactc tttggtgatg ttgcacatct cgatgatata ctcgtccata cagtcactcc
     5221 tatgctgttt tttcctgcca aggacatgcc ttttgatatt tgcatatatc ggatcatact
     5281 aggacgccgc cgaaagacgg ctataataaa aactgcgctc ggcacagttt gcgcgattca
     5341 atatttttaa g
//
LOCUS       DAWBSB010000152         5340 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_2777_length_5340_cov_2.122990, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000152 DAWBSB010000000
VERSION     DAWBSB010000152.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 5340)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5340)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5340
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2777_length_5340_cov_2.122990"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1077 a         1677 c         1330 g         1256 t
ORIGIN      
        1 catataccgc atctgcgccg tacagcacgg caaatttcag ccgttccagg tcgccggccg
       61 ggctcaaaat ttcgggccgt ttcattcaat cacctcaaaa atgcagccgc acacaggagg
      121 tgcggctgct tcgcttattt tattgccttt tcttcaagat cacggacgga tcccctggct
      181 ctccaggtcg gccgtgattg cttcatgctc ggcatttgtt ttggcaaaat ggtaattgcc
      241 cagtgtgtcg gtctggaaat acaggtagtc gcagttttcc tccggccaca gcgccgcttc
      301 aatggacagc aggcccgggt tcgacaccgg tcccgcaggc aggcctgtat gcgtgtaggt
      361 atcgtacgcc tcgcgcagct ccgccggaat cgcatcccag ctgccatagc catagggccc
      421 ggccatggag tcataaatat agttgttgtc gtcggccttt tctttgtccc acgcaacgtt
      481 gctgcccagc gtcatgccgc tggcaaggcg gttgtggaac actgcggaga ccattttgct
      541 gtactcgttt cctgcttctt cctgcacgag gctggccagc gtaacaacct ctgtcagcgt
      601 catatccaat tcatccatgc gtgcatacat ttcagcagtg atcttattgt cgaattcgcc
      661 gtatatcttg gcaaccatgt tgtacacggt gtctcccaca aagaattcat aggtgtccgg
      721 gaacagatac ccctccgctt tcatgaactg gttgggcttt tcctcgcgct tggcccagaa
      781 gccgaactgg gaaaaatctc cattgttggc cacgtccaga aattcctgtg cggaacacaa
      841 acccgcctgc tccataattt gtgcgtattg gatcaccgtt tttccctccg ggaatgtgac
      901 ccgaaccgtc tctctgtcat ctgtagcgac ctgcagtgcg gcgatgatct catcataact
      961 catatcggag gacagttcaa acgtaccgta ttgcagtgta tccgccgcac cgtccttgcc
     1021 gcgtacataa aaacggaaca cctgcgccga gcgaattatc cccgcatcct gcagcttctg
     1081 gccgatggcc agcgggccgg accccttttc aatggtgacg ctttgtgtga cagccgcacc
     1141 gcgtgagccg ctcacctcgc cggacagcca gaaccaacct gctgctgccg ccagtgccgc
     1201 cagcatcaaa aacaccagca tcacgatcag gcatccgtgt tttttgcgtt ttttccgtct
     1261 gcgtgcgggc acttcatctt catcctcttc ttcatactca tagccgtccg catactccgg
     1321 ctcatatgcg gcatcagcag cgggcggctg ctgcaccggc gcagcctgcg gcacgggcgc
     1381 ttccggcgcc aacgaacgca gcttttgagc atacagcccg cgcagagcag gcggcagcgc
     1441 cgccacacgt ttgttcagct cttcgatttt tgccgcattg cgctgctttt gctcctccgt
     1501 catgtgcagc acgcgcgccg aaagctcggc cagttgccgc acataagcct cttgtgcggc
     1561 ctgctgggtt ccttctttgt tttcttccat attcgtcacc tctttgcaat cagtgccaaa
     1621 acgtccttat ggatgtcttc cttagcgcgc atccgcccgc cttccgtgca agccacgcgt
     1681 ttccatccaa gtgcctttgc acagtattgc ccggcctggc ggctgtgctg caggtattcc
     1741 atattttttt cgtggatgtc ctttttttct tcgtctccat gatacctctg ggacatcagc
     1801 cgctgggaga cctccgggtc cacatccagg tatacgacgg tatccggttc cggtatggca
     1861 attttgcgat attcgaagtc aaacaaccag gcgagataag cgtcccactc ctcgcgcggc
     1921 agcttggaac attgatgcac cgcgttggag gtggtataac ggtccgctat cacggttccg
     1981 ccgtcgtggt aaaagcaccc ccaaagtgtc ttatagcttg cataccggtc cacagaataa
     2041 aagctggacg ccgcataggc gttcacatca ttcacatcct gcccaaaagc cccgcccaga
     2101 tacattttca ccagagcgct ggaattactc tcataattcg gaaaggtgat cttcatcgcc
     2161 cgttctccac gtccgcacaa tgcctcgtag agcagccccg cctgcgtggc tttcccacta
     2221 ccgtccagcc cttccagcac gatcaaacgt ccgctcaaac catttcgtct cctttgagaa
     2281 aagcatattt cttccgcatc tctgcggcac gcccgcagct cattgaaccc tccgggcatg
     2341 cgccggatac acacggcggg ccgctgcgcg caaacagtgc aggggccacc tcattcacca
     2401 ggcgcagcat ttcgtccgcc agctcccgaa tctcccactg ggcgcgctgg cagcagcggt
     2461 gccggaagaa attgttcagg cttcgtgcgt tcattgtaac aatcatcttc gtgctgcagg
     2521 cattgggcag cacaaagcgc gcgtcttcaa tggccatttt ttccgccttg cgcgcggccg
     2581 ttttttcatc cgcgccctcc tgcatcaggc ggcggatgtg cccgtctttc agcaactccg
     2641 cgatacggcg gtagttctcc gcctcctccg tcattgccgc gtcaaaggcc tctctggcct
     2701 gttcgtcccg agccacttcc ggcggcgtaa tatattcaaa ctggtccagc cgtacatacc
     2761 gctggctctg tacgctgtac gacgcgatac ggtggcgcgt gatctgtgca agcagtgcgc
     2821 ggctcacgcc ttcgattgca aacgtaaagg atgcgtgttc tgtggggctt tcatgcccca
     2881 ggctggcgag ctttgaaagg aattcccggc ttttttccgg cgtaaggccc tccaacaggt
     2941 cgtccaggtg cgcattggaa taacacagct ttgccgcagc cgcgacgacg cgttccggct
     3001 ccggcgtgtg tgcaatcagc attactttca tggcttgtcc tctcccgccg gcgatttctc
     3061 caacacccgg atgggaaggc cggaggaatc gtacagcact tttgcatggt ttatttttat
     3121 gcaggtgcgc tcctcgcaaa cgcccgtttt cacgttgatg cgggagcggc atattcggtt
     3181 gttgcggtaa acgctttctc cttcacagga aaaatattca tcctccgcat gcacatggta
     3241 tttgtacggg agcgcatgtt ccgcgcgaag ctcgccgcac aaataaatct catctgtatg
     3301 aatcaaaagc tgaaacgttt gttccgtagg gttataaaaa cggtaatcga gatagttgta
     3361 caggatcgag gtccccgttc cgaatgggat cgtccgcccg aaatccggaa aaagatcata
     3421 tccgtcatga tggtggtgtt ccgtgatgac gagcggcgta tgcagcacca tccaatgaag
     3481 cagattcgta aactggcaca tcccgccgcc cacgccgcag gctgtttctc ctttgctgat
     3541 tgtcaggccc ggtaaatatc cccggcgcgc agtacacggc cccaccatcc gccagaacga
     3601 aaatgtttct cccggacgaa tgagcactcc gtttactctg ggtgcggcaa gcgatagatt
     3661 aacagcttta ttttcctgca gcttctggtc caccgtccca agcgtgcgcc gtatcagtga
     3721 cttatgggca tacactacga caggcagcgg atcacaccgc cgttgccggg aaaaacgcac
     3781 accggaaaac gcatcctgca cacgccgcac aaaacggcac tttttcatgg agagccaata
     3841 agcccacggg cccatctcgc aaaaaagcct gcgcgttttt tccattcctg ttttcagacg
     3901 gaggccggaa cgcaaaagga cattccggcg ctccgtccgt cttaaagacg tttttactcg
     3961 gaaatatgtt tcagcggcgc ataattcgcc atcgtctttt ttacaccggc cgtatcaaaa
     4021 cgaatttcaa cgatggtatc tcccgccacg ggcgttacct tcagcacctc gccgcggccg
     4081 aaaactttat gctccaccag gtctcccgga cgataggctc cgctgccctc cgttcgcgga
     4141 acggatgccg cgctgcccgc ctgaaccgtg gaaaaaccgc cgcccacaga agcgcgctgc
     4201 cgggcaggac gtgaagcgcc gcccgctcca gagctatggg aagcgccgcc gtaacgggcc
     4261 gaatatcccg cctgtaccgt ggagcgcatg cttgccgcgc catattccgg ctgcaaatat
     4321 gcattttgct gctgcatata acgctgacgc atagccttca tctgcgcggc agccgggctt
     4381 tgctccacct ccaccagaga ggcgtcgatc tcctccagaa aacgggaagg gcgattgtgc
     4441 tttgtctggc cgaacagcat gcgcgagttt gcacaggaaa gataaagctc ttttttcgca
     4501 cgggtaatac ccacgtagca aagcctgcgc tcttcctcca gatcttcatt ggaataccgg
     4561 ctcatctcgc tggggaatat tccttcttcc agaccgacga tgaacacata attaaactcc
     4621 agccccttgg ccgcatgcat ggtcatcaga acgacaacat ctgcgccttc atcataactg
     4681 tcgatatcac tgatgagcgc cacttcctcc agaaagcccg gcaggctggc ttccggaccg
     4741 cgctgatcgg cataggtctt tacactggaa acaagctgac cgatgttttc catgcgcgtc
     4801 tggccttctt ctccctctgc ctccagcata ctgcggtatc ccgtgatctc caacatctcg
     4861 ctgacgaaca catccagagg cagcgtatcg tacgcctcct tcagcctgcg gtaaatgcgc
     4921 caaaagccgc caagcgccgc gcttgcccgc gccagcgccg gataatccgg cgcgtgctca
     4981 acaacctcca gcattgagac gccaagcccc gccgcaatat ccgccacgtt ctgcacggtt
     5041 gtcgcgccga tcttgcgcgc cggttcgttg atgatgcgcc gcagccgcag atcgtcctgc
     5101 tcgtttgcga cgatgctcat ataagcaagc atgtccttta tctctttacg gtcgtagaaa
     5161 cgctggccgc cgacgatttt atacggaata cccgcacggg caaaataggt ttcgattggc
     5221 ccggactgtg cgttcatacg gtacaggatc gcatggtctc gcagcttcgc gccctttttt
     5281 acgttctggc cgatcaccgc cgccacatgg gccgcctcgt cgaactcaga atccgcttca
//
LOCUS       DAWBSB010000153         5325 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_2788_length_5325_cov_3.689753, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000153 DAWBSB010000000
VERSION     DAWBSB010000153.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 5325)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5325)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5325
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2788_length_5325_cov_3.689753"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1165 a         1500 c         1532 g         1128 t
ORIGIN      
        1 caatactttt tgacgaccgc gagcgctgga atcgtcggta tcccatgcgc tgtttcgtgc
       61 ggcttccatt tcccggcgta ctgcttctgg cgtggtccca tttctctcag caattgtttt
      121 agtcaattct tctaaatgtt tgctgtccat tctatttcct cctttcttta ctataatagt
      181 ccaattgcga ctaaatttta atagtcacaa taagactagg gtatagtaaa catgggtgaa
      241 ggcaatggat ttgacgcgca tccgcgcgct gcgagaggat aacgataaga ctcagcagga
      301 aatagcggac ttgttatgca ttaagcagac cacatattca aagtatgaat tgggccgtgt
      361 cgcgattccc atcgacgcgt tgatgacgct ggccgatttt tacggggtat ccttggatta
      421 tcttgcgggg cgttccgatg aaaaaggaaa ataaaaaagc caacgttctt cgaagagaaa
      481 aacgctggct tttgtttttt aaagctaatt attgcacatt ggccgtgcac gccgtttttt
      541 tcagggagag gaaaaacacc acaagccctg cgcccaggca gatatgtccc agccccgcga
      601 cgccggagat tgccgcgtcc atcccgcggg aaagcggaag cgccagtacc tgcgccgcgc
      661 cgcgcacggc cagcattacc gccgtcacgc aaagcccggt attgtacagc ccgtagaaca
      721 gccggaagtt tctttcccgg gcccagtcaa gctctttgga aaacagcgcg gcaagcagga
      781 aaaacaccgt tcccagcagg aacaggtgcg tgtgtaccag gcccagcgcc gtgcgcccgg
      841 aaaacccgtt gaattttgtg aattcacggt agaatacgcc gccggccatc gcggccagag
      901 cgtaaatgaa tgcggtgttc agcaattttt tcatttgaca gtctccttcc ttttacgatg
      961 catatccgaa aagcccatcc agaccaccca cacataagcg caggtcttgg ggatcatgag
     1021 catgcccacg agcggaacga cgtcggccca cagcaccacc gggatataga acgcgaagct
     1081 gagcacgatg gccagccaca tccagcggaa ggccctgtcc cccgcctgcc gcgcgccgcg
     1141 caggaatacc acgatgacca gcgcgcccag cagtgcgaac ggaacgttgc gccagatgcc
     1201 ccaggacagc ggcgggttcg ccgaaagcca cgcattctgc gggaacaggc acaatccgat
     1261 gcgtgccgcg gccagcgcgt atacggcgcc cgtcagcgcc cggatgtgca ggccatagcg
     1321 ccgttttcca atatggtaaa gaataacgta aaatattgtc attgtaatgg acgtgatgaa
     1381 tttgcccgct cccagtgctg ccgcgtgggc cgccaggccg tcggtacaca gcgcccaggc
     1441 ccggggcacc agatgaaatg catcgccgca gcccagaatg acggccatcc agccgaataa
     1501 tttcgtttcc cggtcctgcg cgctttttac catgatacac cccagcgtca ccaccgtgac
     1561 aagataggcg gcgtcgaaca gagtttccat aattgcctgc atatgcttct ctccaatctg
     1621 aacgatgttc attttaatga gtgtatttcc ccgcacccgg ctgcccgggc acggggaatt
     1681 ttcctttttc agtgcaaggc ccgcactgcc tgcatcaata aatcaggcgg cagacgactt
     1741 cctgcaaaaa cgaggcgtcc tctctgtccg cccgcagcat cgacatcatc gtgccgaata
     1801 cgaaggatac gaattgctca cgggtgaaag cctccgtaaa tgcgccgggc cgcacacggg
     1861 catccctggc aagtacagcc agcatgcccg ctcgaatgtg ctcccaatac tgcatttcca
     1921 gcgcttttcc ctgctggcgc tcggcctcgt tcaggcttgc cagctcccgc aggaaggtgt
     1981 gctcaaactg ggccagatgc gtgcgcagcg cggtatagat ctgcccgcat acggcgctga
     2041 aatccgagcc atgggtaaaa tcacaggaga ggccgccgtg gaacacgcgt ttccaaaaat
     2101 cttcaatcac agccagcaca aggtccgcct tggcgggata ataattgtat acagtgccga
     2161 cagacacgcc gcacatggcg gccgtgcggc ggatgctgat gtgtcccagc ccgtcccgcc
     2221 gcgcgatctc ccgcgcggct tccagaagcg cctgccggga cgtcgcagtt ttattcatcg
     2281 agttcgacct ccaaactgaa caatgttcat tttattccct ttctttccgc ctgtcaatcc
     2341 cttttccgca atttgccgcc aaagaatccc ctttgttttg cggcccgaat cgtgtataat
     2401 aaaaagtgcg cggcggcgtt tgggtgccgg agcaacgcgc ggcgcccgcg cagacagtgc
     2461 gggcggtgtg tggaaaggga ggcgtctgtt ttatgaaaaa agaaaagcag caaaagaggc
     2521 cgaagcgcac gaaagcccag cgcatacagg cgctcaagac ctttgccgtg gatacggtat
     2581 acgatgttgt gggcagtatc ctgtttgcta tcggcgttta tacgtttgcc aaatcagcgg
     2641 attttgcgcc cggcggcgtg tcgggcctgg cgctgatctg caattatttg tgggatctgc
     2701 ccatcggcac gatgaccctt atcatcaaca ttcccatcat catcatcagc tataaggtgg
     2761 tggggcgcaa gtttctgctc aagagtttca agacgatggt catttccacg ttgtttttgg
     2821 accttgtgtt cccgctgacg ccgctgtaca cgggcaacag tttcctggcg gccatttttt
     2881 ccggtgtgtt tatgggcgca ggtctggcgc tgatctatat gcgcggctcg tccaccggcg
     2941 gcacggattt cctcatcgtc accatcaaaa agcttaagcc gcatttctcc atgggccaag
     3001 tgacgctgat gacggacctt gttgtcatcg tgctgggcgg ttttgtgttc ggcaacatcg
     3061 acgcagtgct gtacggcgtt atctccacct atgcggcgtc ggtgatcatt gataagatca
     3121 tctacggcgc gggcagcggc aagctggcca tcatcatcac caccgacggc tatgccactg
     3181 cgaagaagat cgatgaggaa acgggccggg gtgcaacgct tatcaaggcc atcggtacgt
     3241 attcgggcat tgagcggcat cttattctgt gtgcctgcgg caaggcggag atattcaagg
     3301 tgcgcaacgc ggcccatgca gtggacccca acgcctttgt gatgatcacc gaggccagcg
     3361 aggtgttcgg cgagggcttc accccgccgg agctggacca atagcccggc agaaaagcat
     3421 attttggctg cgctccgtgt tttcggcggg gtgcggcctt ttttgtgcgg cacgggagag
     3481 tcccggcggg cggtatgccg ccggctttgg ttaccccgca caaaaagtgc agaaatagtt
     3541 ctacaagatt tatgtgcgga tgagccggaa ttctcttgat agacacaata tatggtgtta
     3601 gaatgaaaag cgtatgataa ttggagaatt gaagaaaggt gaggaaaaga aatggcgatc
     3661 gaagacattg ggaaaaatgc gctgcaggtg ctggaaaagc ggtatctggc caaggacgcg
     3721 gagggcaacc acacggagga tgtggcgggc ctgttccgcc gtgttgccgc ggccgtggcg
     3781 gcggcagacc ggaattatga tgaaaacgcg gatactgctg cgctggcgga cagcttttac
     3841 gagctgatga ccagcctgcg ttttttgcca aactctccca cgctgatgaa tgcgggccgc
     3901 ccgttggggc agctttcggc ctgctttgtg ctgcctgtgg aggacagcat ggaggcgatt
     3961 ttcgagacgg tgaaaaacgc cgcgctcatc cacaaatccg gcggcggcac gggcttttcg
     4021 ttctcccgtc tgcggcagaa cggcgcgccc gtcaattcca caggcggcgt ggcctccggc
     4081 cccgtcagct ttatgcgcgt gttcaatatg gctaccgagg ccgtgaagca gggcggcacc
     4141 cggcgcgggg cggacatggg catcctgcgg ggggaccatc ccgatatcct ggaattcatc
     4201 gactgcaaaa aggtgaacgg ggacatcacc aatttcaata tttcggtggg catcaccgag
     4261 gcgttcatgc aggccgtgga ggaaaaccgg gactacgacc tcatcgaccc gcagacccgg
     4321 cgcccggtgg gcaaacgcag cgcccgggag ctgtttgaaa agatcgtgga cgcggcatgg
     4381 cgcaacggcg agccgggcat catcttcctg gaccgcctga accgggacaa tgtggttcca
     4441 tcccaggggg agatcgaatc caccaatccc tgcggtgagc agccgctgct gccctacgaa
     4501 agctgcaacc tgggcagcat caatctggtg aaaatgcttg caaaaacgcc ggaaggctat
     4561 gccgtggatt acggcctgct gcgggacact gtgcgccagg ccatacattt tttggacaac
     4621 gttattgacg tgaataacta tccgctggag cagattggcg cggtgacacg ggccacacgc
     4681 aaaatcggcc tgggcgtgat gggctttgcc gatatgctgc tgtatctggg ggtgccctac
     4741 aattccgacg agggcgttgc gttggcggag aagctgatgg cctttatcaa cgccgagggg
     4801 cacgcggcca gtactgcgct ggcggcccag cgcggtgcgt tccctttgtt tgccgagagc
     4861 atctacaagg atggcgatct cctgcgcaac ggcaccgtca ccaccatcgc gcccacgggt
     4921 acgctgtcca tgatcggcgg ctgctcctcc ggcgtggagc ctgtgtttgc ctatgtgtat
     4981 atccgcaaca tcatggacgg tacggagatg ctggaggtaa acccggtgct gcgggaaaag
     5041 ctgcaggagc gcggactgta ctccgacgct ttgatgcgca aaatcgccaa agaaggtacc
     5101 atcgcccata tcgcggagat tcccgaggac atccgccgtg tgtttgtttg tgcccacgac
     5161 gtaagcccgg tatatcatac caggatgcag gccgctttcc agcggcacac ggacaacgcg
     5221 gtgtccaaga ccgtgaattt tccccactcc gccacccgcg aggaagtggc tgaagtgtat
     5281 atgctggcct ataaaatggg ctgcaagggc gtcaccatct accgg
//
LOCUS       DAWBSB010000154         5290 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_2805_length_5290_cov_3.341929, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000154 DAWBSB010000000
VERSION     DAWBSB010000154.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 5290)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5290)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5290
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2805_length_5290_cov_3.341929"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4715..4724
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          996 a         1769 c         1481 g         1034 t
ORIGIN      
        1 ggctttccgg ctctgctgct gatgacgctt ccagcctcat gcgcgcaaag cgcgcgtggc
       61 ggcgtaagcg gcggccttga ggttattata ccacgatgcc gccgcaggcg gccacaacaa
      121 aaccgcactt gctgcggttt tgcgcggttc aatgttctca aagccctgtg cgttagcgca
      181 aaagcgagtg cggcgcacac gcgcagccgc ggcagcggcg gctttgagat tattataccc
      241 caaacgccgt caggtttgca agaggctccc cgccttgccg gagcctctga ggcaacagag
      301 agaaagcagc ggccgggcac cgctgctttt atcaaccgta aagggcttca ttcattttgc
      361 gaagggaagc gtccaacgcc gtgccacgcg ctcaggcacg cagcaaaaag caccggtcgg
      421 ccaaaagcgc cgtccagtac acggcgtttt tatagcgcag cgtcacccag gtaaagccgc
      481 cgtagcaccc caccgactgc gccaccgcct cgtaccggcc cacaggaagc gtacccgcca
      541 cgtcataaat atccggcttg taaaaatact cacagttaag cgtcccatat acctccagct
      601 tcaggccggg caccggcacc atctccagcc cgggtatctc aggttcatag ccattgtagc
      661 ctcccgcctg aatggccggc agaaaatcca catacgaatg gtccaggtcc acattgcccg
      721 atatacccgg tacacgccct gtgctggaat actgccacat ctggtaagcg cccggatagg
      781 taacgctgcc cgtgtaatcc gccacaaaaa acggataagc gctgagcttc gccatatcca
      841 gccggttttt tgcaaaattg gaataggtgt acaggcccgg ataccacccc gccgcacgca
      901 gtttttccag tgcgtagagc gcaagctgcg tcgccctcgc ccggcccagt gcggcgatgc
      961 cctcggcctc catatctaca aataccggat attccagctt cttgcccgcc agaactgccg
     1021 tcagcgcctc cagctccctg tcggcgtagc tctctgtttg ggcataggtg tagtaataaa
     1081 cacccacccg aagcccggcg gcctttgccc ccgcatagtt cttctcgaac atggggtcta
     1141 catacacgcc ggaattgttg gaggataccg cgcggataac ggcaaactgc ttgcccgccc
     1201 gggccaccgc ggcccagtcg atggtaccct gataacggga tacgtcgatt cccgtttgat
     1261 aaatagctgc cataaaaacc tccctgctgc cgcacaggct gtgccgggcg gcggccgcct
     1321 ctttcggcgg gcggctggcc ggtctgcagc ggagcgcggc atgcgccccc tgcgttcatc
     1381 ctatgcgtat ccgcagcgtt ttgtccgcgc ttcgggcgca tgatgttcgt ttgcacagca
     1441 agggcgtaca tgctatactg aaaaagcacg gcgctccaac aggacagcgc cgcatcggga
     1501 ggcttttttg tggagatcga tatcagacgc gcggctcagg aggatctgga tgccgcggag
     1561 cagctttaca acgatgtctg cagcgctttg gaggccggta caaactggcc cggctggcgg
     1621 caaggcgttt accccacccg cgccgacgcc cggcgcgccc aggcggccgg ggcgctgtac
     1681 ctcgcctggg acggtgcccg cgccatagga agcatggtgc tgaatcacga cgccgacccg
     1741 gattatgccc aggttcccgg ctggggaccg gacgcggact ggccgcacct gctgctgatc
     1801 cacacgttgg ccgtgcatcc cgcgtatcag gggcggggcc tggcccggcg gctgctcgcc
     1861 catgcggagg tccttgcgcg gcaaagcggc atgcgtgcgc ttcgcctgga tgtctgtgtg
     1921 ggcaacgtcc ctgccatccg cctgtatgag cagtgcggct ttgcgtgcgc cggcacagcc
     1981 gagatgcact tctgcgtcgg caccgtcgac ccgtgccgcc tgtacgagaa aacgctgtga
     2041 cacgggacag cacgcggaaa gaaggccgtg cgcacccgga cgacccgcgc gtccgtattt
     2101 tgctgcaccg catcgccgca gcctgccgca acaccctcgg cgacacgctt gcgggtgttt
     2161 acgtccacgg ctctctcgct ttcggctgct tttgttggac acaaagcgat atcgattttc
     2221 tcgttgccgt gcgaaggccg ctttctcagg cgcaaaagga ggccctgctc cgcgcgctgc
     2281 tggcgctgga gccggacgcc ccgtccaaag ggctggagat gagcgcagta ctggaggcat
     2341 actgccggtc ctttatccac cccgcgccct acgagctgca ttattcgccc atgcaccgag
     2401 cccgctgcct gcgtgacctc cccgcttact gcgcggaaat gcatggcgca gacccggacc
     2461 ttgcggcgca tttcaccgta gcccgcgccg tcggcctcac cctttggggg ccgcctgccg
     2521 aaagcatatt cggtcctgtg ccccgcgcgg cgtatctgga cagcatccgc caagacgtgg
     2581 gcaccgcctg ttcggacatc ctgcggacgc ccgcctccac cacgctgaac ctgtgccgaa
     2641 cgcttgcttt tgcacaggaa gggctcgtct tgtccaaaga acaaggcggg ctttgggccg
     2701 cgtgccgtct gccgccccca tatgccggct tcgcacaaag cgcggcggaa agctaccgtt
     2761 ccggcgcgcc ttttctgccc gcgcccgcac aggcacaggc attcgcagcc tttcttctgc
     2821 cgtccatctt ccacccggac ttcttcgggc aaaacatttg agcacaagcc gggatacgac
     2881 agtgaaaaga gcgtttcccg caggctgtcc cgcctgtgga aaacgctctt tttatctcct
     2941 gttttaccgt tcagccgccc cgcagcgcgc gctgtgccac accctcggcg gcttccagat
     3001 ccgcgctgcg cacataaaca taccagatgc ccgcatccgc ggagtgcgcg ttcgtcatgc
     3061 ggcggtcggc cccggtgtag ttgcgtccgc tgcgcacgat gcattcaaag ccgataccgg
     3121 cctgctccag tgctgtgcgc acggcgtaaa gctgtgggcc gtcttttccc atatacacct
     3181 ctctgcgctg ccaaaaccac atgcccgctc ctttccctcg cagccgctca ccgcaccgca
     3241 gcgatgcgtt caatctcgtc cagctcctgc tgggtaaacg gcgcggcgtt tactatggcg
     3301 gcgttctcca cgatctgctg cggacgggac gcgccaatga gcacgctggt cacgccgctg
     3361 tccttgagca cccacgaaag cgccatctgc gccagcgtct ggccccgggc ggcagccagc
     3421 tcgttcagcg ccgcgatctg tgccagccgc tccggcgtaa gggcgctctc cttcaaaaaa
     3481 acaccgcttt ttttgatgcg gctgtctgcg ggcacgccgt gcagatattt gtccgtcagc
     3541 agtccctgcg ccagcgggct gaaagcgatg acacccacac catgggccgc ggcatacgcc
     3601 ttcagcccgt ttttttcgat ggtgcggtca aagatggagt accggctctg attgatgatg
     3661 aacgggcaat gcagctcttt caggatagcc gccgcccgcg ctgtcgtctc gccgtcgtaa
     3721 ttggagatgc cggcgtacag cgccttaccc tgctgcacgg ccgaagccag cgcgcccatg
     3781 gtctcctcca gcggggtctc cgggtccatc cgatgggaat aaaaaatatc gaaatactcc
     3841 agcccggtgc gcttgaggct ctggtccagg ctggccagca gatacttgcg gctgccgaaa
     3901 tccccgtaag ggccgggcca catggtatat cccgctttgc tggaaagcac cagctcatcc
     3961 cggtagggac gcaggtcttt ttcaaaaata cggccgaaat tctcctcggc agcgccgggc
     4021 acagggccgt agttgttcgc catatcgatg tgcgtgatgc cgcagtcgaa tgcggcgcgc
     4081 agcatggcgc gcatattgtc gaaggcgtcg cacgagccga agttgtgcca cagccccagc
     4141 gaaacgcggg gcagcttcag gccgcttttg ccgcagcggg cgtattgcat cgcctcataa
     4201 cggcgctcgt tggcagtgta aggcatggtt tggtccccct tttgtctttt cttttatttt
     4261 gaattccgcg gcggacggcc cgccagcaga tatgcgaccc aggcaaacgg cgcaagcgcc
     4321 accagcagca cccaggtgcg gtaattcagc gtgcgcgttc cgccgtggcc cctctggtac
     4381 agcaacacgc agatgcgcgc caaaatatcc gccagccccg ccagagcgac gatcagaatc
     4441 tgttccgtag ctttccctcc ccggcgatat tcttgcctat ttgttactgt actgccatat
     4501 ttccaggctc gcacttccca tcctgcccgt gttcactgag aaacatggta tcctacgccg
     4561 tcaagcgcat acttcgcctg cggctcatat ttgctaaaca gccatattat ggccatgacg
     4621 ccgctgcaag gcggccttcc acaaaaccgc acctactgcg gttttgcatg gttcaaattc
     4681 cccaaagttc tgtgcgccgg aacagcagcg tgtgnnnnnn nnnnctcatg cgcaagagaa
     4741 aaaacgcggc acgccccggc taccgggaag ccacggcatg cgcatgcccg tgtgcgtttt
     4801 cgaaaaaacg acggacattc ccccgcgcca aacgggcatc ccccggtaag aacgggacag
     4861 cagctttgcc caaaccgttc ccggtggccg aaaaaggccg aaaccggctt ttttccgcct
     4921 ttgcgccccc aaagactatc ctgtataata tctatcatac cgcggggccg ctgcaaaatg
     4981 gccccgcggg cagcagaagc gctccccatc cgtcggcgcc agcggcggga gcgcttccat
     5041 ccttccgcac ccgtaaacac aggaagcatt gcctcgattt ctctcccgca aagggcagcg
     5101 gtcgctctca cgcaccggca gaatttttat tgttaaaaca cgtcgaaccg tatgcgccga
     5161 aagattcagc ttccttcctg ctgccagcaa gcttcccatt gctaaaaacg ccgctgaacc
     5221 gcatgcgccg agggattcag ctttcttccc gctgccggca agtttcccac tgttaaatac
     5281 gccgccgaac
//
LOCUS       DAWBSB010000155         5271 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_2811_length_5271_cov_3.280100, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000155 DAWBSB010000000
VERSION     DAWBSB010000155.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 5271)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5271)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5271
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2811_length_5271_cov_3.280100"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    636..645
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2411..2420
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5022..5031
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1061 a         1557 c         1608 g         1015 t
ORIGIN      
        1 gagatcatgt cctccacaga ggccgttttg ctctcgaatt tttttgcgat gtagaccacc
       61 agccgcaaat tgtgaacgat aagtacatcg cggcagtcgg ggtttccggc ctccaggccg
      121 gcaaaaacga ccttttcctc ttctttgctc agcggcggag gcagcgtttc cggcccgttg
      181 atataataag cgtagtccgg caggcggatg cagatgatga atttcctgca cagggccgcc
      241 agccccgtgc gcagtgatgc aagcagtggc atttttcttc tcctttgtat ggtctgcagg
      301 cggcgcggcg ccctgtgcgg ccggaagctg cgtctttgtt cgcccggcgg ctgcgtcagg
      361 ctccgcaaac ccctccctcc gcgggtacga tggcgcagaa gccgccgtcc gccagaggct
      421 ccggcgtgaa caccgcacac acgcccgtat agcggcgggg cgcgccgtct gcctccaaca
      481 ccagttcacg tacgcgcacc gcaggcaggg cacaggtgcc cgccgccgtt ttggtgggga
      541 tgagccgcag cggcatgcct cccgtctcca tggcgccgtc gtcgaaataa agcgccagcg
      601 cccgcagcag cccaggcggc agcgcgccgc gcaccnnnnn nnnnnggtga acggtatggt
      661 ccgcacctgc tgcggccggc cgaatgccca tgcgcaaagc tgtacggcca gatatacgcc
      721 ggcaatgcac ccgataagca gcaaagggga aacattgaaa tacaccgaca ggttattggt
      781 gctgacattc tgcgccgcac catagtatgt agccgcaaat accgcgcctg aaagcgcaat
      841 gttcagaaac acataccaga agcagcattt caggaagccg cgcacattgt gcaccgggaa
      901 gctggcgaat acgatgagca ccgcgctgcc gatttttgcc ccccacatga gccattgcgg
      961 cagaggaggc aaaagcagca gcagcgaaga aaggcccgcg gcggccgccc ccagaaagat
     1021 acgccaatcg gcggcgatac gcccggccag cagcgccgcg ccccgcagca gaaaatatcc
     1081 gatgagggca ttgaccaaaa acagaatatc cacatagatc acaccgcgca tgccgccgcc
     1141 ccctcttctc cgagtgtagt acacccggag cgaaaaacat gtcaaaccaa aggggcgcgc
     1201 gtcgcaggtt ttgtatgcgg gcttgtagca tgggccgttt ctgcctcgta atgataggca
     1261 aaggacattt ttcacgttcc aaatacagcg cggcgcgccg cgcggaaaaa ggaagcatac
     1321 gaccatgaag caaaaccgca aacgctctga acgccgccct gtggaacggc tttgccccac
     1381 accggaaaca gggcttacag aggaacaggc ggcccagcgc cgggccgccg gatggaacaa
     1441 cgccgcgcag gacagcctct ccaaaacaga atggcagatc gtgcgggaca acgtatttac
     1501 ctttttcaat tttgtgttcg tggcgctggc cgcctgcctg ttcgctgttg gcgcataccg
     1561 cgacatgatg tttttgggca ttgtggccgc caatgtgttc atcggcattg tgcaggagct
     1621 gcgcgtcaaa cgcacgctgg acaaggtgac actgctctcg ggcagcacgg cttgtgtggt
     1681 ccgcggcggc cgaacgcgca ccgtccccac agacgagctg gtactggacg acattgtgct
     1741 gctgcgcccg ggcagccagg tgtgcgcgga cgccgttgtt gcggacggga cgctggaggt
     1801 aaacgaagcg ctgctgacag gcgaggcaga gcttgtgcaa aaacgccccg gggacacgct
     1861 gctctcgggc agttttgtgg tgaccggcgc gtgcgcggca cggctggata aggtcggcac
     1921 cgactgttat gccgcgcgca tcaccggcga ggcccggcgc cgcaaacgtc accgctcgga
     1981 gatgatgcgc gcattggaca ggctgctgcg tttcatcagc attgccatca tccccgtatt
     2041 cgtcatcatg tttgcaaagc agtatttctt tcttcacaca gggctggagt acgccatgag
     2101 ttccaccgtg gccgccatca tcggcatgat ccccgagggg ctttaccttc tggtgagcgt
     2161 agcgctggcc gtgagcgtca tcagcctggc gcgcagcaaa acgctggttc acgagctttc
     2221 ctgcatcgag aatctggccc gggtggacac gctgtgtctg gacaaaacag gcaccatcac
     2281 cgagggctgc atggaagtgc tggaaaccat ccccgtaccg ggcatggagg gcggcgcact
     2341 ggcagagctg ctgggcagct ttaccgccgc cgcgggcgag gacaacgcca cggcgcaggc
     2401 tctgcgggca nnnnnnnnnn gcgctttggg cggcggctgg cagcccaaag ccgccgtccc
     2461 cttcagctcc gagcgcaagt ggggtgctct ggtgctgaaa gacggagacg aatacatcct
     2521 cggcgcaccg gagatcgtgc tgggcggcgg gtatgccgcc ttccggggta cggtagagcc
     2581 gcagctggcg gcaggacggc gcgtgctggt gctggcgcgc ggacacggcg tgctggaggg
     2641 cggtacgctg cagcacgatc cgcaggcgct gggctttgtg gtgctttcgg ataaaatccg
     2701 tgcggatgca cctgcaacac tggcctattt caaggcacag ggcgtggatg taaaggtcat
     2761 ctccggcgat aacgcggtat ccgtggccga agttgcgcgc cgggcgggcg tggagggcgc
     2821 ggaacattat ctggatatgt cccgcgtacc tgaagacgcg gaccttcgcg gtgcggcgca
     2881 ggcgcatgcc gtgttcggcc gtgtgacgcc gcagcaaaag cgcgcgctca tctgcgcgct
     2941 gaaggacacg ggccgcaccg tggccatgat cggcgacggg gtaaacgacg tgctggcttt
     3001 aaaagatgcg gattgcagca tcgccatggc ggcaggcagc gaggctgcgc agcatgtggc
     3061 ccagctggtg ctgctgagct ccaatttttc tgccatgccg cagatcgtca aagaggggcg
     3121 gcgtgtcatc aacaatatcg aacgttcggc cacactgttc cttgtaaaaa acatcttctc
     3181 attcgttgtt tcgttggttc ttctgtttgc ggccatgccc tatccgctgg tgcccgcgca
     3241 gatcagtctg ctcagcgggc tgcttatcgg cgttccttcg ttcctgctga cattcgagcc
     3301 atcctatggg cgtgtacgcg gcagctttat gcgcagcgtg ctgctgaacg cactgcccgg
     3361 cgggctagtc aatgtggtga acctgctggc tgtgatgtgg atcggctccg ggatgggcct
     3421 tcccatggag cagatttcca ctgtgtgcac actgctggca ggcacggcag gatttatggt
     3481 gctgctgttt ttgtgctggc ctctgtccag atggcgcttt gccatcgtgg ccgccatggg
     3541 cgtgggcttc atcggcgcat gcgtcctgct ggggccgctg ttcggccttt cccctctttc
     3601 agggcgtgcg tggacgctgc tgggcgtatt tgccgccgcc actcccgcgc tgatgtgcct
     3661 attcgtgttc cttgtgagcc gcgtcaagca gagactgcgc cgttcccagc gcgccaagca
     3721 tgccgccggg ctgtctgcca cactttcctg aagcacattt ttctttattt cacagcgcgc
     3781 tccgccttcc gaaacatatg ccggaggttg gagcgcgttt tctctgcccc gcaccgtccg
     3841 cagcttgaat tcaattcaaa aaaacctcat ctgcgttcaa catccgctcc attgtatacg
     3901 cggcgctcgt atgttacgat ggaaccgggc ccaaaagaaa cgaaaggaat ttgccgaaat
     3961 ggaaatgcca accggaaaaa ccatccaaac actgcgcaga caaaagggac tgacgcagga
     4021 acagcttgcc gaggccgtgg gtgttaccgc cgcggcggtg tccaaatggg agacggccag
     4081 tgcctacccg gatattctgc tgctgtgccc tctggcgcgg gcgttgggca ccacacccga
     4141 catactgctg gattttcggc ctcttctttc cgaggaagaa ctgaaagcca tctgggaaaa
     4201 aggacgcgtc ctgtttgaaa gcggccgcgc cgaagaagcc tgcgcctatt gtgaagatat
     4261 tctgctttcc catccaggcg acaccgcgct caaattccgc atcgcatcgc tgtatatgcg
     4321 ctatatggcg gccctgaaag acgaagcgtt cgtacaaaag caattagaac gctccatccg
     4381 cctgtttgag gacagcacgc acagcacgga caccgagacc cgtgaggccg cattctacac
     4441 gctgtccggc ctgtatatga tgaatggaga agccggtcgt gcgctggacg ccgtagaaaa
     4501 actccctcag cccgattacg acgcccgcat gctgaaagca tccatcctga gacagacggg
     4561 acaagcggag gaagcggaaa agctgcacca gacctgcctt tggggatatg tgcgggacgc
     4621 gggactgtgc ttatacagcc tgtccgccga ggcacgtgcg cgcggggata cggagaaggc
     4681 cctcgcactg ttggacgcag cctgccgtct ggacgttttg tttcatggtg aaaacgcgcc
     4741 cggcatgcac ggaaactatg agctggcacg cgccgaattg ctggctctgg cgggcaagac
     4801 gccggctgcg ctggacgccg ccgaagccta cgcggaaagc gctgttacgg gctgccgcgc
     4861 gcaggaatat tcgccgctgt ttttcgaccg gctctcctcc tccggcatca tggatgtatc
     4921 ggatgatttt ctgcgtgaaa acgcgctgtt cgtactggag agctctgaac cgctgcacgc
     4981 actgaagcag gagccgcgct atgccgcgct gctggagcgg tnnnnnnnnn ngccgcaagg
     5041 cgaattaggc agccggcaaa tgacagaatc acgcccaaag catggtttcc gaagacgcgg
     5101 cgcatcctgc cggaatggac agtgcgtaaa agatttataa aaaaactccc ggcaagccct
     5161 gaaagtttgt tgttgattag ccatgcagtt atgctacaat atggataatc aataaattta
     5221 aggcaacacc gcacaattct aagagtcgaa tcgcgccggc agattttttg g
//
LOCUS       DAWBSB010000156         5270 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_2812_length_5270_cov_3.131927, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000156 DAWBSB010000000
VERSION     DAWBSB010000156.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 5270)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5270)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5270
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2812_length_5270_cov_3.131927"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2842..2851
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4357..4366
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1119 a         1654 c         1431 g         1046 t
ORIGIN      
        1 gtttcttgca tctaatacaa agcattcaga ttatttcctt tcctgttctc cgcatcttac
       61 agaaacggat tatacgcgga gaaaaatgag taaaaacagc tgcccgaaag cgacgaatcg
      121 ttgacgaacg gcagatttcc gtttgtgatg accaccacgg cccacagcag gcacaggatg
      181 agcagcacgt tgatgaaccc ggccaccacg ccgatacaca cgcccagaat gcggttcacg
      241 ccgcccagaa gcggaatctt attgatattg gtgagcacag tgacaaggaa tgcaatgacg
      301 acacggcaca gcacaaacgt ggcaaaaaac accaccacgg tgatgagcgg caccaccaac
      361 ggcgcaatga tcttttctac cacctgctga gcaatatccg gagcgccgga atccagcatg
      421 ccggccgcag aagccgtgat atcgtcgatg aacttctgcg gcaggatgcc cgaaaggccg
      481 tccagaatga cggacaggtt caccccgccc tgctgctgga tggtacgggc cgtctgttca
      541 atgaggcccg atttgaaaaa gttttcaaac accgtgggcg aaagcttgcc ggataccacc
      601 caggccagcg ccaaagacgc gaggttgccc acaaggtcca tcaagcccgc cacaaagcct
      661 ttgcgggcgt aatggaacac agacacgacg atcaccgcga tgagcgcgac gtcgatgatg
      721 attgccggtg agatattcat aagtatgtcc tttcctgcgg gatgcattcc cccgcatatg
      781 cttcggttgc ggtagccggc gtcatcccgc ggacgccgac ggcggcgcgg gcggctcgaa
      841 cacgctcact tccccctgct ccagcaaaag cagctttcgg ccgttcacca tcgccagcgg
      901 ctttgcctcc gtcacggttg cgccgtaaaa cacgccgtcc aggccatagc tcagcacacc
      961 gtcatttgtc agaacaaacg caccggtacg gctggctgcc acgccgcttg cgcgctcgtt
     1021 cacgtcggca ctgcccgcct cggacagggt cgcgtccaga atcgtaagct gcgtcaatgc
     1081 gccgtgtacg ccgtcgccaa acagcagcac cgcattcttg cccgcgctga tggccgcgct
     1141 ctgcagcgtg cgcccaccgt aggaatagcg gtacaactct gttccgtcgg ccgtatcgta
     1201 caccgcggca taagtatcgt acagcaccag cacctcggca ttggtgaggt actggatctt
     1261 cacgggcatg ccctcggtat tgcgaatggt gacatattca tctttacgtg tatccatcag
     1321 gtacacggta ccgcccagcg cgccgccttc ggcgtaaggg caccccgctg cgaactgccg
     1381 gttgtcgccg gcaaacgcga gagctgtagg caggtcctgc gtctggaagg tatagatgtt
     1441 ctcaaacatc gggtcataaa catacagctt tgactttgta aaaaccgcca gcgtgccgtt
     1501 ggggctcata gcgcagagct ggatggcgtc atcaaatttg ttttcgaaca gcgtgcggct
     1561 gcggctctcc acccgcagct ccttgccagc gcggttgtat acacacacac gtgtgttgcc
     1621 cgccgtaatg gccggcctgc cgtaaccgtg ctggatgcgc cgcacctcgt tgccgcccgc
     1681 ggaatagatg gccaggtcct tatcgcctac cgccacgaag ccgcctgcca gcggcagcgc
     1741 attctgatac ccggacagcg taaatgaaac gggatagccg gggctgggcg tcagcgcgat
     1801 gccgatggag tccaccgcat cgcccagcag cgccagcgac gcgccgtaaa tgccggtgaa
     1861 ataggccagt accgcagcca aaagcaccgc gcacaccgca gccgcgcgga tacggcgcat
     1921 gcgcttgcgc cgccggatac gttcaatacg tgccgcgctg ctccgaggcg gcacgggctc
     1981 cgcgccgggc gcctgctctt ggatggcgcg cggctcaccc gcatcgggac gcaccgcact
     2041 ccgcgcaggg gcgccgggcc tctgcggcgt tgtattctcc tgttcgcgcc cgtctggttc
     2101 gcgtggcatc agttccataa atcgtatact ctcttttgcg tttcgttatt cctgtttcgc
     2161 cgttatttcc tgcgcggcct gtgtattgcc ggcttcttct tcggagtaat tcaccttgta
     2221 aaggaaaagc ccctgcgccg gagcggtatc ccccgccagc gaacgttttt tttcttccag
     2281 aatatcccgc acgcgctccg gcggcatgcg ccccgcgccc gcttccacca gcgtgcccac
     2341 aaaaatgcgc accatattgt acaggtagcc gtccgcgcac acatggaacg tgatatcgtc
     2401 cccctcacgc cgcacatggg cgaaatacac cgtgcgcacc gtatcggcga tatcgctgcc
     2461 ggaggacatg aacgacacaa aatcgtgttt tcccacaagg tactgcgccg cgcgttccat
     2521 cctgccggca tccaaaggcc ctgggaaacg ccagcacaac ccgttctgaa acgggtccgc
     2581 cacggcgctg ttatgaatgc ggtagatata ttctttgctt ttcgcactgt aacgggcgtg
     2641 gaaatcctcc ggcacccggt gcgcctccgt cactcgtaca tcgtccggca aaaagcggtt
     2701 gacggcaagc ggaagcttgc gcggcggaat cggcttgggc tgcgtgtaac tgacacagta
     2761 cccaagcgcg tgcacaccgg aatccgtgcg cgaacaccct ttcaccggcg gccgcacgcc
     2821 gtacaggctt tccagcgcgt tnnnnnnnnn ncccgtggta gcgcgtgccg gcaaagcgca
     2881 gcgtaatgca gacgtgcata aaattcctcc cggacgattt taacacaaac cccttttacc
     2941 caatgcgtgc aaaaaacagg ttgctcatac caatggcgac aaacaccagc accgccacaa
     3001 cagcaatgag gatgtcggaa cggtgcatgt gcagctgctt caggcgggtg cgcccctcgc
     3061 cgccatgata gcagcggcac tccatggcca tggccagctc gtctgcccgg cggaacgccg
     3121 aaataaacag aggaatcaac actggaatca acgcctttac acgctggcgc agattgccgg
     3181 aatccagctg cgcgccgcgc gccttctggg cgttcataat cttgtccgtc tcttcgatca
     3241 gcgtgggaat gaaccgcaga gcaatggtca tcatcatggc aagctcatgc accggcaagt
     3301 gcagctttgc cagcggatgc agaagctgct cgatggcgtc ggtcagcaca atagggctgg
     3361 ttgtgtatgt cagcaggctc gtgcccgcga tcagacagat gatgcgcaca gccataatga
     3421 cggcgtaata gataccctct ttgtatattt tcaatatcca ccaggacacc agcggctcgg
     3481 atgttcccgt cataaaaaac agattcagca cggccgtaaa cagaatgatg ggcaggatcg
     3541 gcttgatgct ttttcccacg attttgagcg gtatcttcgc cacaaggtac aaaacagtga
     3601 ggaacacgat ggaaataacg agtcccgccg gattcgaacc aacaaacagc atcacgatat
     3661 aggcgatggt gagcagcagc ttcacgcggg ggtccattgt atgcaggatg gaattgcccg
     3721 ggaaatgctg gccaatggtg atgtcacgca gcatttacat accctccttt gctgccgcac
     3781 gggcggcaag gatggtctta acagcgtatt ttaccgtata tacgtcggtg tcgatgtcca
     3841 gccccatctc acgcaggcgc aaaaatatct tcgtcacctc gggcaccgaa aggcccagct
     3901 ccagcagctc cggcgcgcgg gcaaacacct tatctgtggc gtcgtacagc gcaatgcgtc
     3961 ccgcgttcat caccagcacg cggtctgcat atttggcgat atcctccatg gagtggctga
     4021 caaggacgat ggtggacccg gtctgcttat ggtattcccg tatctgtccg aggatcatat
     4081 cccggccctc cgggtccagc ccggcggcgg gttcgtccaa caccagaagc cgcggttcca
     4141 tggcaagcac acctgcaatg gccacacggc gcttctgccc gccggaaagc tcaaaggggc
     4201 tgcgctgcaa aaagcagctg tcaatgccgc agaacgcggc cgcacgctgc acgcgccggt
     4261 cgatctcctc cgcagaaagc cccatattcg cggggccgaa agcgatgtcc ttatataccg
     4321 tttcttcaaa aagctgatac tccgggtact ggaacannnn nnnnnnctgg aacaccagac
     4381 ctgccataaa acgaaaacgg cggatgtctt tggggtccgc ccacatatcc tcaccggcaa
     4441 tgtacacctt gccggaggta ggcttgttga taccgttgaa gtgcgtgatg agcgtggatt
     4501 tgccgcagcc ggtggaaccg atgacaccca cgaactcgcc ttcttcgatt tcgaagctga
     4561 cgtgatccag agccacagtc tcgtccggca tgccctggtt atagatatag gtgaggtctt
     4621 ccaccttcac gattgcggac atgcggacac ctcctctgct ttttcctgtg cggctccggg
     4681 cgcttttttt cctgtaagca gttcatacag cgccgcagcg cactcttcca catcaatgat
     4741 gtcatccgcc atcgggcagc ccgcagccgt gagggcatgg cacagctcgg cggcctgcgg
     4801 cacgtccagg tgcagcgcac gcagctcctc cacattacgg aacacctctt tcggcgtgcc
     4861 ctccataacc actctgcccg cgctcattac caccacacgg tcggctgtgg cggcctcttc
     4921 catataatgc gtgatctgga cgacggtgat gccgtagtcg tcgttgagtc tgtgtaccgt
     4981 acgcatgacc ttttcgcggc cgatggggtc cagcatcgcg gtggcttcat ccagcacaag
     5041 gcaatccggc cgcattgcga tcacgcccgc gatggccacg cgctgcttct gcccgccgga
     5101 cagcttgtgc gggccctttt ccctgtattt atagatcccg gccatctgca tggcctcgtc
     5161 cacacgcgcc cgcatttccg cagggggaac acccagattt tccaacgcaa aagcgacgtc
     5221 ctcctccact acgttggcga cgatctggtt atccgggttc tggaacacca
//
LOCUS       DAWBSB010000157         5255 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_2817_length_5255_cov_2.794231, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000157 DAWBSB010000000
VERSION     DAWBSB010000157.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 5255)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5255)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5255
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2817_length_5255_cov_2.794231"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1620..1629
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3881..3890
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          993 a         1534 c         1662 g         1046 t
ORIGIN      
        1 cttggaccgt gtggggaaaa agctgctggc caccggcaac ggccgctgca acctgacgaa
       61 ccggaacgcc gcgccggaac attatcacag tgcggatacg gcccgtttgg cggatatatt
      121 ggagcgaacc cctccggacg cagtgctgga cttttttggg gagatgggcc ttttgtgtga
      181 tacccaggcc gacggaagag tatatcccta ctgctatcag gcatccatgg tgctggatgt
      241 actgcgtgcg gcactggagc gtgcaggcgt ggatgtggcg tgcagctgcg aggtggcgga
      301 ggtggaaaaa gggcccggcg gtttttcggt gcgtacgcgg gacggccggg ccttttcggc
      361 cgcgtgtgtg gttttggctg cgggcggtca ggccgggccg cagttcggca cggatggttc
      421 cgcttttgct ttggcgcgca tgctgggcca cagtgtggcg gagccgtatc catgccttgt
      481 tccgctgcgg tgcgcagggt ttcccgtcgg attgaaagga gtgcgggcca actgtgcgct
      541 ttcgctgtac ctggatggcc ggtgcgccgg aaccgaacgg ggagaagtac agttcgcaga
      601 atacggcctc tccggaatcc cggccatgca gctttcgtgc ctgcttggcc cgggcgtgaa
      661 gaaggcgggg acaagcgtgg atttttttcc ggagatggcg tttcctgtcc tgcgcgggat
      721 gctggagcag cgctgcgcgt cgggcatgtt tgcttcgctg gagacgcttc tgctggggct
      781 cgtggccaaa aagctggggc atgcgatact gaaaagctgc ggcctgcagc cgctttcgcg
      841 cgcgccgggc agcctttccc gcagggaagt gaacgctctg gccagtgcgc ttaaaaactg
      901 gcgctttgcc gtagagggca cgctgccttg gacgcaggcg caggtgacgg gcggcggtgt
      961 gccgctggcc gaggtgaaga cggaaggctg tgcctccctc aggtgcccgg gcctgtatct
     1021 ttcgggcgag gtgctggacg cggcggggga ctgcggaggc tttaacctgc aatgggcctg
     1081 ggcaacggga atcgcagcag gccgggacgc ggcgcggggg ctgtaaaaaa acaaaacaca
     1141 cggggctgac ggaaacagcc ccgtgtgttt tgtttcggtc attgattcgg tttgcccgtc
     1201 atgcggctgc cgtccggcgc atccagttcc agcacaaggc cgctgaacgc gggcaccgga
     1261 aatgtcagca cgtggccttc ccgagcggcc cgcgagcgca ccgcaggcgt ttcctccggc
     1321 agagcgccgc caaagcgctg ccactccgtg tgcagaagca cccgggcacg gcaggcgaag
     1381 ggcatgtgca ggcggtaact gggcgcgggc gtatcgccga aattgaatgc tgcggccacg
     1441 gtgtgcgtgc cgcctctgcg caggaacgca tacaggcagg tcccgtcggc ttggcagtcc
     1501 aaccattgaa aatgtgcggg atcatactcg ccctgccaaa aggcgtcgtt ggaaagatac
     1561 aggcggttca gctcttccgt aaaatggcgg aacgcatcgt ggatggggta ttgcagcagn
     1621 nnnnnnnnnc agcatccaat ccagttcccg ggcttcgtcc cattcgcgca tgtggccaag
     1681 ctcgctgccc ataaagttca gctttttacc cggatgcgcc atcatataca ggtacagcgc
     1741 ccgcgcctgc ggaaattttt gttcgtaatc tccgtacatt ttctgcacga tggctgcttt
     1801 gccgtggacc acctcgtcgt gggagagggg gagaagatac tgttcctgcg gaaagtatgc
     1861 catggaaaac gtcagcttgt ggtactgtgc ccggcgctcc tccggcgttt ttttgaagta
     1921 gtccagcgtg tcgttcatcc agcccaggtc ccacttgtag tcgaagccca gcccgccgta
     1981 ttccacagga gcagtcacct tggggaaatt ggtggaatcc tctgcgatga gcatcgctgt
     2041 gggatgcaga cgccgcaggc cggcattcat tgtctgcaaa aatgaaacgg cattcccgtt
     2101 cacaccgcgg gcggggtcgc cctgccagta gatcatgcgg ctgatggcgt ccatgcgcag
     2161 gccgtcgaag tggaactcct ccagccagta gtgtgcggca gactgcaaaa agctgcacac
     2221 ctctccgcgg gagtgcatga aattacagct gccccattcg ctgacgccca catccgaatg
     2281 gggatattca tacagagctg tgccgtcata attggccagt gcgtatgcat ccagtgcaaa
     2341 atgcacgggt acgaaatcca gaagcacgcc tatgcccgcg gcgtggcatt tgtcgatgag
     2401 ctccttgagc tggtcgggcg tgccgtagcg gctcgtgggc gcgaaaaagc cggtgttctg
     2461 gtacccccag gagcagtccg ccgggtgctc gctcaggggc atgaattcaa tatagttata
     2521 gccgttttct tttacgtggg caatcagctt gtccgcgatt tcatcatagc ggtaccatcc
     2581 gttttcgcgg gcctcgtctt ttttccagga gccgaaatgc atctcataaa tattgacggg
     2641 cgcatcgaaa gcgcggctgc gcgccgccat ccaggcttcg tctgtgaaac ggtacccgcc
     2701 cagaggccag agcgcggagg cgctgcccgg acgaagttcc atccaaaagc cataggggtc
     2761 gcaatggtcc gcctcaccgc ccgcagcggg acggattttg tatttgtaca acatgccggg
     2821 ctttgcgccc tgtatacggc aggaaaatac gccgccctgt ccctcccggg ccatgggcat
     2881 accctgccag ccgttgaatt ctccaatcag ctccacagcc tgtgcgccgg gcgcataggt
     2941 gcggaaggtt acgaagtctc cctcaaaatg cgcgccgaaa aaacggtgcg catcgaatgt
     3001 acggccggtg taaaaatcgt gcagatccat ttctggcggc ctccccatga tgcttttttg
     3061 ttctttcatt ataggcggtg aaggaacggg aagtcaatcg acaatgcagg gaggttgtcg
     3121 ggtataaggc gtgtcaaata aaaagttcgt aaacgcccag cgccacaatg agcgccgccg
     3181 agcatactcc cgcatacctg cccagatgcc cgccgccgaa ccgcctgccc acagcggcgc
     3241 cgcccgcaag aagtatcagg ctgcacacga acgtgcatgc tgttgttttg acaatggaaa
     3301 gccctgtgat gcgcgcaccc acgccaaacc ccacattgtt caccatcagt gcggcggcaa
     3361 gagcggcact ttcccgcagg cccagccgcg ttccacaggc gtccgcaggc ggctgttccg
     3421 gcgctcccaa tccgccctgc gcctcgcggt acagcatcca caggcccatc aggaccagca
     3481 gcatgcagcc caatgcattt gccgtgtccg gcgggatcag ccgtgccagc cctccgccca
     3541 gcagcattgc ggccgctgta ccgagactgg taaggcatgc gatgagaagg ttgccgcccg
     3601 ctccgatgcg cataccacgc agcccgtaag caatgccgat ggggaaatta tcaatattgg
     3661 ctgaaagcgc gaaaagacac gcagaaatcc actgtgccat gcataacgcc tcctttccgg
     3721 gaaaaagccc ctgtcatcag tatatggccg ggccgggatt tggtgcgcgg tgggaggcag
     3781 gaagcttagg catgtggtat aataacctca aagctgccgc ttgcgcggct gcgcgtgtgc
     3841 aaggcacacg cccctgtgcc ggcgcacagg gctttgagaa nnnnnnnnnn gcaggtgcgg
     3901 ttttgttgag gccgcttcgc ggcagaagga agggaaagac gaaatgatca tcagcgcgag
     3961 ccgccgcacg gatattccag cgtgctacgg acagtggatg atggcgcgcc tgagggcggg
     4021 agaggtgctt gtgccgaacc cgtataacgc gtcgcgtatc agccgcgtgg ccctttcgcc
     4081 gggcaatgtg gactgcatcg ttttttggac gaaaaacgcc gcgcccatgc tgccgctgct
     4141 ggatgagctc gacgcgcttg gatacaaata ctattttgaa tatacagtaa cgggctacgg
     4201 ctccgcactg gagccgggcc tgcctgaaaa agcgcgcacg gtggatacgt ttcttcgtct
     4261 gtccgagcgc ctcggcccgg agggcgtgga ctggcggttc gacccggtgc ttcttacttc
     4321 ctccatcact cccgcgtggc agttagagcg tttcggtgcg ctgtgctgtg cgctgcacgg
     4381 agccacccgg cggtgtgttt tcagctttgc ggacccatat ccgggcattc gtccgtccat
     4441 cgccccgttg gaggaaacgc agatgcgctt gctggcgaaa gggtttgcag acattgcggg
     4501 agagtatggg ctgccgctgt acacctgtgc cgaggctgtg gagctttccg aatacggcat
     4561 ccggcacgcg gcgtgcatcg acagcgcgaa agtggaacgt atcgcagggt atcccatccg
     4621 ggtagaaagg gatcccggcc agcgcccggc ctgcggctgt gcggcgtccg tggatgtcgg
     4681 cgtatacaat acctgtccca acggctgcac ctactgttat gccaccacac gcccggcgct
     4741 gctgcggcgc gcccgcgccg tccatgcacc ggacgcgccg atgctcaccg gatggccgcg
     4801 gggaggcgaa cagatcacag accgcacggg cccttcgctg cgtgcggaac agacgtcgtt
     4861 gtttgacaac ctttaaaagt caaaaaggaa ggagacccgg agatgggtct ccttcctttg
     4921 agggggccgc caaagccgca gccctatgag aataccaaac gctttgtacg gcgctgccgc
     4981 tcattgtttt cctcggttct cctttccggc gcacaagggg gaagcttgag cgcttcgcac
     5041 acaaatcacc cttttgtgct gcaaggtgtt tgcccggccg gcgtagctcc gggcgggcaa
     5101 acgattttta ttctttttca agtggacaga cagcttgccg aaaaagctgc gcttttgcgc
     5161 cgggctgccg gcgaacaggg ctttcggccc gttcgccgca cacggcggtc ctcggcggtc
     5221 gtttcgctct acacgcctcg gcactggccc tgcgg
//
LOCUS       DAWBSB010000158         5229 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_2831_length_5229_cov_2.749710, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000158 DAWBSB010000000
VERSION     DAWBSB010000158.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 5229)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5229)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5229
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2831_length_5229_cov_2.749710"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1124 a         1407 c         1649 g         1049 t
ORIGIN      
        1 ctatatgata gccataatag caattagccg ttatatagta tgaaccggct ccatgcatta
       61 cctaaattcc agcgtccatg aggaaatgcc ttttcttcgc acaaaagcat ttaaatcatt
      121 gctaaacaaa tatattcttc cggacatttg ttgccggact tttgcttgtg tgcattttct
      181 tttcgcggca gttccccgta ctcaatataa ataattcaca tcaaaatcat atcacgtttt
      241 aagcatggaa aaagacgcag ctttctcttg atggaagctg cgtctttttg gtgcgggtaa
      301 caggggtcga acctgcacgt cgtagacacc agatcctaag tctggcgcgt ctgccaattc
      361 cgccataccc gcgcaatgta tttattttac caatccggcg gggcgtttgt caatgccctg
      421 ccgtcagcat tcggtgatca ggcgcgcggt ggcttcgata aaggcgtctg tgtccagcgc
      481 cgggtttttc agttggcttt gcaccagcat gccgtccatc acagtcagcg tcagccaggc
      541 caggtattca ccgtccgcac cgggtttacg cgccgcgatc ttctgcgcca gtacgttctt
      601 aaagtgagca tacttttcca gcagcttgtc ccgcacatcg cctttgcccg aaaccgcctc
      661 cgcaatcaga aaaagacgca tgctgccgct tttgtcgtat actccgcggg aaaatgtata
      721 gcgcagcagg cggggcaggg aggtatcctt ttcttcattg tctacccagg caagaaggtc
      781 gtccgagagc ttttgcagat accggtccgt caaatcgaac aaaatagcgt cccggctgtt
      841 gtagtaatag tacaacgttc ccttgctgat gcgcgcggcc cgggcgatgt ctgccagtga
      901 gatgtcgaag aacgagcgtt cctccatcaa cgcggccgcg ctttccagaa tgcgttcccg
      961 tatttgtgtg ccaagcggtg cagccatggc gatcgccccc atctttttcg tctaatacaa
     1021 tagtataaaa gatgctgggc acaatgtcaa agaaagttta catttcccgt acaaaccgta
     1081 cattgacatt ccctgcggcg ggtgctatcc tgaaaacgga agaagtcggt cggttgaccg
     1141 actgataaaa tcggcaggaa agcgaagggg agacgaatgc ggataccgct caaaaaaata
     1201 aaccgtgcgc tgaaaaagcg gttcggcggg cgggtgcagg ctgtggtgga gcgcggcgtg
     1261 ctggtgctgc gcggcagtgc ggaggattgg gacggcgtgg ttgcggcggg acgtcttgca
     1321 gccagcccca aaagccgctg gtatacggta aacgaggtaa catggccggg tgcggcgcaa
     1381 agacagccaa agcctgtgcc gcagacggat gaaagcctgg ccggacgcgc gccggacgtg
     1441 ctgctcatcg gcgcgggcgt tacaggtgcc gccatcgcgc gggagctgat gcggtggaat
     1501 gtcgacctgc tctgggtgga aaaagagcac gatgtggcgc tgcatgcatc aagccgcaat
     1561 gacggcatgg tgcatccggg tgtagatctg cataaaggta cggccaaata tcattacaat
     1621 cgtttgggca accggatgta tggcggcgtg tgtgccgcgt tgggcgtgcc tttccggcgc
     1681 acgggacagt acctgtgctt caagggcaga gcaatgcgcc cgcttctgta ctttacgctg
     1741 ccttattgga aatggctggg cctctctggt gtgcgcgtgc tgggggacaa agcgctgcat
     1801 gcagccgagc cgggtgtgga tgaggcgctg cactgcgggt tgttttttcc acaggcgggc
     1861 attgtctgtc cttacgggct gaccattgcc atggcggaga acgccgtgca gaacggtgcg
     1921 cacctttcgc tggatacatg cgtgacgggt atggacgtgg agaacggacg aatccttgcc
     1981 gtgcatacaa accggggtac agtgcgcccg cgcatcgtca tcaatgcggc gggtgtgttt
     2041 tgtgaggata tagcgcagat ggcgcaggat cattttttct ccatccatcc gcgcaaggga
     2101 acaaatgcca tcctggacaa aaaggcgaag cgcatggtac gcaccattta ttccggcatg
     2161 ggaactaccg ccaccaagac ggtccattcc aagggcggag gcatcgtttc tacggtggac
     2221 ggcaacgtgt tggtggggcc cgacgcggtg gaaacgccgg aaaaagaaga tttttccacc
     2281 agcgtgcaaa gcgtgcgcgc cacgtttgaa aagcagcgcc atgccgcacc tgcgctgcgt
     2341 gaaagcgata tcatcaccta ttttacaggc gtgcgagcgg cgacgtatga ggaggatttc
     2401 atcatacggc ccgggatgtt cacggaaaat atcatccatg cggcgggcat ccagtctccg
     2461 gggctgacgg ccgcgcccgc catcgcgcag gaggtggcgc ggctggccta tggacagctt
     2521 tccgcgacaa ccccgcccgc gcgcaatgcc gcctacgacc cgacacgcag gcccataccg
     2581 catgtggcgg ggctgccgga cgaggcgagg gccgcgctca tccgccaaaa cccggattac
     2641 ggcgagatcg tctgccggtg cgaggaagtg agcaggggag aaatattgga cgcgctgcgc
     2701 aggccggtgc cctgcgatac ggtggacggt gtcaaacggc gtgtgcgccc cggcatgggg
     2761 cgctgccagg gcggcttctg cggcccgctg gtgacagcca tcatcgcgga ggaaaaaggc
     2821 atatcgcttg aacaggtgcg caagtccggt gcgggcagtg cccaactgct gcgccccacg
     2881 aaacagagag aacaaaaaac gggaacggaa ggaggcacag aggatggagc gctatgacgt
     2941 cgtgatcgtg ggcggcggcc cggcggggct tgcagcggcg ctgggcgccc ggcgagcggg
     3001 ggcaaaaacg ctgctggtgg agcgcgaggc acggctgggc ggggtcttga agcagtgcat
     3061 ccacgacgga tttggcctgc tgcgcttcgg ggaacggctc tccggctgcg aatatgccca
     3121 ccggtacatt gaaatgctac gcggctgcgg cgcgcagacc gcgctgctga ctttcgtttc
     3181 ccgcatcgaa aaaagcgggg aaggctttgt gctcacgctg gtgggcaggg aagggctgcg
     3241 gaaggtggag gcccgggcgt tggtgctctc cacaggctgc cgcgagcgca cagcccggca
     3301 ggtgtccata catggtaccc gtccggcggg tgtgctcacc gcaggtacgg cccagcatta
     3361 cattaaccgg atgggggaga tgcccgcccg gcgctgcgtg atccttggtt ccggtgacat
     3421 cgggctcatc atggcccgcc ggctgacgct ggagggcgct caggtgttgg gcgtatatga
     3481 ggcaaagccc acgcccagtg ggctttcgcg caatatcagt cagtgcctgg aggattttgg
     3541 tattccattg tacaccagcc ataccgtcac ccgcgtttcg ggcaaagagc ggttggaacg
     3601 cgtgacggtg gccgaggtgg acgcgcggat gcgtcccata ccgggcacgg aacaggagct
     3661 cgaatgcgac agcctgatct tatccgtggg gttgatcccg gaaaatgagc tggccgaggc
     3721 actcggcgtg cctttggatg cacgaacaaa agggcctctg tgtgaccaga gctatatgac
     3781 gctggtgccg ggcgtttttg tgtgcggtaa cgcgctgcat gtgaacgacc ttgtggatta
     3841 tgtttccgaa agcggcgaag cggcgggggc ggcggccgcg cgctatgttc ccgcggcgtg
     3901 cgggttggcg gaggtgtcgg cagacggcgg gtttggatat gtggtgccgc agcgcattga
     3961 cacgacaaag cctgccggga aaacaacttt cttcttccgt tcccttgctg aacgcggaaa
     4021 aacgcgtgtt cgtatgctgg cggacggcag agaactttgc aaaaagacat atatgcagct
     4081 taagccgccg gagatggagc ggatggtggt ggacctttcg ggggccgggc tgcatgcagg
     4141 ctcccggctt acattcgtga tggaggaggc ggacacatga agcgggaatt tacttgcatc
     4201 gtctgcccca acggctgccg cttacacacc gaagtggaaa acggcgcggt gacgtctgtg
     4261 acggggaacc gctgcccaaa agggcgcgat tttgccgaaa aggaatgcac cgcccccacg
     4321 cgaagcttgt gcaccacggt aaaaacgctg gggggcggcg caccggtatt gccggtgcgc
     4381 acagcgggag aattgcccaa ggaacgcctg tttgaagcca tggctgtact aaacgcgtat
     4441 acgcagaccg tgcctgtata ctgtggagac gtcctcatac cggacctttt aggcacgggc
     4501 tgcgccgtta ttgccacgga cgattgggcg cctgaggcgg cggagctgaa ataaacggga
     4561 actctttgcc gttccatccg acataaaggg aaccagcaga cctgccgcaa gacgaacttc
     4621 ccatttttaa aaaggaggag acgaccatgt ccaagccata tcgtgatttt gaacccaaat
     4681 ggtaccacgg cgaggtacca cccacatcct accgctccat tttcaaatgg ggcgcgccgg
     4741 atcagatcaa accgcccaag gaatcgcttt ataaactcgt gaaggaaaaa tttggcctga
     4801 cggacgcgga ctttgaccac tatacggaaa atttaggcct ggacgcagtg cggttcgatc
     4861 tgccctgcac catggaacaa cgccgcctgg atgcgtttcg ggacatcgtg ggtgcggaat
     4921 atgtgcgcac ggacgattac gcgcgccttt ccgttgcata cggcaagacc atgtacgaca
     4981 tcctgcgcct gcgcaatcaa gtggtggaaa acgtaccgga cgccgtgctt tatccggata
     5041 caaaggaaca gatcgagcga attgtggctt actgcgcgca ggaaaagata ccggtttatg
     5101 tctatggcgg cggctcgtcc gtcacacggg gcgtggagtg catgaaaggc ggtatttcgc
     5161 tggatatgcg cctgcgcttc aacaaagtgg ccgccttcaa tgaagtggcc cagaccatca
     5221 ccgtagaag
//
LOCUS       DAWBSB010000159         5118 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_2906_length_5118_cov_2.254790, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000159 DAWBSB010000000
VERSION     DAWBSB010000159.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 5118)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5118)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5118
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2906_length_5118_cov_2.254790"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    367..376
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3647..3656
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4750..4759
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1122 a         1372 c         1534 g         1060 t
ORIGIN      
        1 ccatgcagat gatttacgaa tacgatgacc agggcaaccc caaaatgcgg gaatacgaac
       61 tggttgttgt gggcgtgatg aagactgact actccaaacg cggcaccgag ggcatcatca
      121 tgaatattgc cgatatgaag cgcctggagg aagagtataa aaaactgagc aaaaattccg
      181 gtatgggcgg cggcagcgtc gtgatgtacg gtggctccag cggtaccaag gtgaaaggat
      241 acgaccaggt atacgttaag gtggacgatg tgaaccatgt gggcgcggtg gagcagatga
      301 tcaaggatat cggctaccaa acttcctcca tgacgcagta cagggaggat atgcaaaagc
      361 aggtagnnnn nnnnnngatg atgctgggtg gccttgcggc ggtttcactt ctggtggcgg
      421 cgctcaacat tgccaatacc atgacgatgg cgatttacga acgaacaaaa gagatcggcg
      481 tgatgaaggt gctcggttgc aagctttcga agatacgcca gatgttcctc attgaatccg
      541 gcaccatcgg ttttatcggc ggtgttatag gatgtctgtt tagtgttttg atcagctttg
      601 ttctgaataa ttttacggtt tggatgcagg ccatcaccgg gtggctgatg agcatcggcg
      661 tgcaggtgaa tttcgatgct tccagctttg acgtcggcgc gttgttcggt atgggcggca
      721 tgggcggtat cggaaacatt tccgatattc ctgcttggct gctgttgatg gcgctgattt
      781 ttgcgacggt ggtgggactg ctttccggca ttgctccggc caatcgggcc gtcaaaatgt
      841 ctgcgttgga agccatccgg cacgaatagc agattatacc cgaaaaatgg acagagaaaa
      901 gacacctcct gttgtagaat aaaaactaca acaggaggtg ttgttgtgtc gagacaatat
      961 gcaaaagcag aagggttgaa cggggaagta tttcgaaaga aagcggcagg agaaacaaat
     1021 cgggaaaaag cagaaagcta tgggctgcaa accattgtct ctttttatac attttcaatt
     1081 tgcgaaacgt atttaccgaa ccctgagtac acccaattct acaattttaa acgcattgaa
     1141 ttggaaacgg cctcacgccc aacccaatca ggagcagggc cgcagaattc ttcgctattc
     1201 tgcgacgagg ctctctactc attgcactgc gctttgtttg atataagcat caggccatgg
     1261 ggttgagcag acggagcacg tcgggcgtaa gcgcgcgcag gatggcgcag gcgagcgcaa
     1321 tggcggcgtc gacatctgcg gcggcagaat agccgtaatg cgtgtgcgca tagcgcacgg
     1381 gaatgcccaa caccagcgtc ggcaccccgc ccggcgcaag gtggatgctg ccgccgttgg
     1441 tgccgccgcc tgtgcggacc gcgcactggt ggggaatgtt gttctcttcc gcaaccctgc
     1501 gtgcaaatgc cacaaggccg ggatgcgcca ccatgctgcc atcgcggaag cgtatctgca
     1561 cacctttttt caaggcgcct tgggcctcgt ccggggcggt gaagttatcg tccgcgggtg
     1621 tgccctccag gcagatggcc agtgcgggac gcaggcgctc cgccgtgatt tttgcaccgc
     1681 gcaggccgac ttcctcctgc gtggaaaacg ccgctgtaac gtcggcacaa agcgtttcgt
     1741 ccttcaatgc acgcagaact tccacaacac cggcacagcc caagcgacag tcaaaagctt
     1801 tgccgagcat caaaccggtg gcgtcgttgt agctgaactg cacgtcgggt accgcaacaa
     1861 ggccgggttc caatccaagc agctctgtgg cgtctttgcg gctgcacact ccggcgtcca
     1921 gcaggatgct ctccatcgaa agcggcgcgc tgcgctcagc cgccgtcata aaatgcggcg
     1981 gcttggaggt ggacacggcg cgcacggtct caccgcgcct gttgtgcagc agaaaggtgt
     2041 gggcggggat gttgtgctcc acccagccgc ccagcggcac gatgcgcagc aggcctgtgg
     2101 ccgtgatgct ctgtaccata aagcccactt catccgtatg ggcgtccagc attaccaacg
     2161 gacgtccgcc cacattctgc ggcaaagaga catacaggtt ctgcatgctg tcgcggtgtg
     2221 cggccagtcc gcccaggctg ctttcaatca catcgaccgc ctctgtttca tatcccgagg
     2281 ggccgaatgc atcggatatc gcagcgcata attcaaggtc agccattttt ttcatacctt
     2341 cttttcattt tttattgccg tggcaaagag cgtaaaatac ggaaagtgcc gcggtctggc
     2401 aaacagcaaa agctgtttgc tgtgaagcat tattcctcca gcgcatctgc cagcgccacc
     2461 cacagcgcgg cgctggcgtc gctgggcatg cgccagtctc cgcgcggcga caatgttacg
     2521 gaacccacct tgggaccgtc cgggaggcag ctacgtttga actgctggga gaaaaaacgc
     2581 ctgtaaaaat tccgcagcca tttcagcagc acggcatttg tgtattctcc ggcaaaagcc
     2641 tgtcgggcaa gataatagac ctttgccgga gagaaccccc agcgcacggc ataatagagg
     2701 aaaaagtcgt gcagtgtgta aggcccgacg atctcttctg tttgttggct gatttttccg
     2761 ttttcagcgg gcagcagctc cggactgacg ggtgtgtcca gaatatcgcg aagcacctgc
     2821 cgaagcgcgc tgctttcagt ttcgtccgcc acggaggcga cgatatgccg aatgagcgtc
     2881 ttggggaccg aggcgtttac gccgtacata ctcatatggt cgccgttata ggtggcccag
     2941 cccagcgcca gctctgaaag atcgcctgtg ccgatgacaa gcccgcccag ccggttggca
     3001 aggttcatga gcaccaacgt gcgcacgcga gcctgcgtat tctcatacgt gacgtcgtgt
     3061 cgggcttcgt cctggccgat gtcggcaaag ttgctgcgca cggtttgagt gatgtctaca
     3121 tgctgcagcg tcacgcccag cgcctcgcaa aggcgttggg cattggtgcg cgtgcgctcc
     3181 gtagtgccga agcaaggcat cgttacggcg acgatgtcgg aaagcgggcg ctccagcagg
     3241 gaaaaggccc gaacggtgac gagcagtgca aggcagctgt ccagtccgcc ggagatgccg
     3301 acaacagcgg ttttagcatg cgtatgttcc agacgctttt tcaggcctga cgcctgcata
     3361 gccaggatgg cgtcacagcg ccgggcgcgc tgttcggcgt ccgccggcac aaaaggagcg
     3421 gatgagacga tgcgggtaag cggagagacg ttttgcggca gtacgaatgg gacgacttca
     3481 tagcttgcgt tgttttcagc ggggaagctg gtgtttttca gccgttcccc cgcaaggcgt
     3541 tccacgtcga tgtcggttaa cgccataccg ccgcagaaag gcgcagtttc ggccagaagc
     3601 gcgccgtttt cagcgaccat attatgtccg gcgaaaacca tatcagnnnn nnnnnnccat
     3661 atcagtggtc gattcgcctt cacccgcatc cgcgtaaacg taaccgcaca gcaggcgggc
     3721 agactggccg gtcacaagcg cacggcggta ttcagccttg ccgatggtct cgtcactggc
     3781 ggaaagattg cagataagtg tggcgcctgc catggcgtgc cgcgtactgg gaggaaaagg
     3841 cgcccaaaga tcctcgcaga tctcgacggc cagcgtcagc gcgggctgcg ttccgcattg
     3901 gaacaacagg ttcgtgccga agggggcctg tgcgcccagc aggtcaatga cgccgttttc
     3961 tgccggggca ggtgtaaact gccgcttttc gtaaaattca ttgtaattgg gcaggtgtgt
     4021 tttcggcacg acgcccagca cacgcccgtt ttggcacaca gccgcgcagt tgtacagctt
     4081 gccgccgtgc gtaagcggca gccccagaac aaaaagaagc ggcagcccgg cggtatcccg
     4141 caggacgtcg gccagtgcgt cccgcgcgcc ctgctgcagc accggttgga aaaacagatc
     4201 cccgcaggta tagcctgtga tgcaaagctc cggcaagcat gcgatttccg cgccggcggc
     4261 ggctgcggcg cgcagcacct cggtgatctg ccgggcattg taggcgcagt ccgccacgcg
     4321 gatgctgggg gtaagcgctg ctgtctttac aaatccatat tgcataaacg atccctcact
     4381 tagttttacg tcatatcaga aaccggaaca accgggagaa aatggaaaca aagcccatca
     4441 aaaggcgctt gccgagagag acattcagcg ccgttgctcc gctccccgga agataagcgt
     4501 tgtccggacc gactgcggcc gattgtgcga acaaagtatc cagctccgca ttcagctgtg
     4561 caaaaaactc cgggctgtcg atgataagaa cagattccgt gtcgatatac aggcttcggt
     4621 catccagatt aaaggaacct accacggaaa ttcgcccatc tgcgacacag gattttccgt
     4681 gaatagaatc tgtgctttga tattcgtaga tttccacgcc ggttgccaaa aacttggagc
     4741 gctgggagcn nnnnnnnnnc ggaaaacgcc ggataattgg gcgaagatgc catggagttt
     4801 gtcagatatt ccacatgccc ttgttttgca aggcgggtga gcgcggccag cgttgacgga
     4861 ttggctgtcg cgtagggcgt ctggagccgg attttttggc agccggagag aagcggcgcc
     4921 agcgtcgcgc ccagtgtggg ttcttttttg aacggggaga caggattgtg cagcagggtg
     4981 acgcgctgtg taggaacggc acattgggag gcaggctccg agggcagata gaacgagggg
     5041 gcggcggcct cccatcttgc ggcagtttcc ttcaagtatt cctgcgccgc aaggccgtct
     5101 gcacggcggc ttgctgaa
//
LOCUS       DAWBSB010000160         5104 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_2916_length_5104_cov_3.720143, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000160 DAWBSB010000000
VERSION     DAWBSB010000160.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 5104)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5104)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5104
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2916_length_5104_cov_3.720143"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1125 a         1425 c         1496 g         1058 t
ORIGIN      
        1 gtgctccgcc agcacgcgca gacgggtgtt ttcgtccttc agcatgtcaa gctcacggtc
       61 caacgacatc cccccatttc atatgtgtgc cgcgccctcc gccgggcatt tctccgggaa
      121 tgttcccgga ttttccgcgt gtcccggacg gtcggaggcg caccttcccc tcttattctc
      181 ttattataga ttcataaatt ttgaccgtca agagtatttc ataaaacagt ctatttttta
      241 cgtgatttta tcttttaaaa gacttttcct gtgttatcct atgattttac tgtgtttaaa
      301 accacctcat ttccagcggc acttgtattg gagggcgcaa aaggattaca ataaagaaaa
      361 acgcccggtg tgctgcgaca cgcgggccgg gagggaattg aaatgacttt gacggagacc
      421 gtgcgcgccc aaacgcgaca ggccgtggcg gagcttctgg cggcagcccg gatgcgcgcg
      481 ggagatattt ttatcatcgg ctgttcgtcc agcgaggtcg tgggccagcg cattgggcgc
      541 ggctccagca tggaggccgc gcaagctgtg tgggaaggcg ccgcgcccct gcttgcggaa
      601 agcggattgt atctggccgc gcagtgctgt gagcacctga accgcgccat cgttgtggaa
      661 cgggcggcgc ttccgccgtg ggcggaaacg gtaaacgccg tgcctcagcc ccacgcgggc
      721 ggttcctttg ccaccgccgc atatgcgcac atggcggacc ccgtactggt ggagaacatc
      781 cgcgcgggcg cgggcattga catcggcggc acactcatcg gcatgcatct gcggcctgtg
      841 gccgtcccgg tgcggctcag tctggcccgc atcggcgagg ccggcattct ttgcgcgcgc
      901 acgcggccga aatacatcgg cggcagccgc gccgtatatc aaacagacga attggaggtg
      961 ccgcgttaaa tgacggcaga agagatcagg cggatggagg aacgcgtcgc acaggtgcgc
     1021 gcggaaatgg agcgggcgtg ccgcgcagcc gggcgcgccc ccggggaggt tcttctgtgc
     1081 gccgccagca aaacgcagaa ttccgagacc gtgcgcgcgg cggctgcgct ggatatcgac
     1141 ctgtttggag aaaaccgcgt gcaggagctt tgtgaaaaat acgccgccgg cgcatacagc
     1201 ggcaagccgc tgcattttat cggccatctg cagaccaaca aggtgcgcca ggtagtgggg
     1261 cgtgcgtgca tgatagaatc cgtcggctcg cacaggctgc tggaatgtgt ggctgcagaa
     1321 gctcagaagc agggcatccg gcaggatatc ctaatcgaga tcaacattgg ggccgaggca
     1381 agcaaaagcg gcatcgcgcc cgaagagctg cccgggcttc tggaatgcgc ccatgctttg
     1441 ccctccctgc gggtgcgcgg cctgatgtcc atcccgcctg ctgcggatgc gccgggcgcg
     1501 agccgcccct actttgcaca gatgcgcggc ctgttcgagc gggcgcaggc cgcgcagggc
     1561 ggcaacgtga gcatggagta cctttccatg ggcatgagcg ccgattttgc agacgctatt
     1621 ttggaagggg caaatctggt ccgcgtcggg accgcactgt tcggcgcgcg gagctatcct
     1681 gcacagtagc tttatataat atgtattata aagggaaaac ggcaccggga tattcaaatc
     1741 ccggcgccgt tttccaaatt cccagaaaca gatcctccct ctgcgtcagc gccccggcgg
     1801 gcggcggcgc ggcggacgcg gacgggcaac gtcatgttcg gcccgctccc ccacatacac
     1861 acaggcatat actgccagcg tgatgggcac tgctaccacg aacaccgctt tgaagatcgt
     1921 atcccagata gtcatcccgc actccccctc cgtcccgctc atgctcctgc ggccggatac
     1981 gctgcctgag ccctttataa cttatatgga catctttgcc gcggatattc attgaaaaag
     2041 gcgtacgagg tggaaagttc ttccttgtgc ggaacggcgg ctgccagaca ggattttgtc
     2101 acaccgctcc tctttacatc cgccgcaggg caaagtatga tagatgcgga gaccgcattc
     2161 gtgcgttaag aaaggagccg gcaatgaaac acgagccgtc gtctgatttg ctgcagtttc
     2221 tgcgcagcaa aaacatcctg ccaaacggtt acttttccct tgaggagccg gacggaacgt
     2281 atacctttta cagcgtatcc cgttccggcg tgctgtacac actggatctg gagccggcgg
     2341 ctttaagtgc ggatgacgtt tgggaaaagc tggaccgtat tcaaaaaatc tcccgggagg
     2401 ttttcgaaca ggcacaggag tcgctttggg acgcacggag gctggcgcgc ggcctgccca
     2461 ccaaccggga actgaagccg gttgcggaac agttttacaa ggactacaca cagcattatg
     2521 cggaaggccg ctggaaaacc gccgcccggt atgacgagga aacgatccgg cacattctga
     2581 atatcgtctg ttcgaatctg caagggggcg gcaaaaatca gcaggccgct tgggacagaa
     2641 tgttccggga ccttgtccag gcaaaggtgt ttcgaaccca acgcgatatt taaaaaataa
     2701 aaagcgcaaa agtattgaca acggcgttat tattggatat actagataag caccttaaag
     2761 aggcgcagac aatttatatc gcggggtgga gcagtccggt agctcgtcgg gctcataacc
     2821 cgaaggtcgt cagttcaaat ctggcccccg caaccatcta tgattacgcc gtcagcttcg
     2881 ttgctggcgg cgtagttcgt ttttacttcc ttcataaacg gcacatcagc attccctttg
     2941 gcccaatcag ccgtggaacg gatgccaatg aggtcttcgt atacgtccac actaaaaata
     3001 tatttgcaca gcggctcctt ggtgcagtcc gggttttcct gtaaggcacg aatgtcctta
     3061 gacagttccc ctgtccgctg cgcccgtgtc acttctgcct gcggtccctt aagccatggg
     3121 cgcagcccgt ggccgattta tggtctgccg tattggtttc cgccctttat cttgcaacgg
     3181 tccaaagaat gtcaagagag gaacggtatc atgaaaggcg cccgagccgc tggcgctgga
     3241 gctcatcctt cacgccaccg cggacaggga gcttgcctga aaagacagga gctgagctga
     3301 ccgcaaggca tcgaacatac cacacaccgt atccagggag gctatatgct gaaaatattt
     3361 ttgtgtgaac ccatccatcc ccgcgcgctg gcgctgctta aaaccagggc tgaggttttt
     3421 tcggaccccg cctgtctggg ccgggccgac gcagccatca gccgaaacct gcgcatggac
     3481 gcagcttttc tggagcagtg cccggctttg aaggtcatcg gcatacacgg cacgggcacg
     3541 gacagagtgg acctggctgc ggcagcgcag cgcggtgtgc gcgtggtata tgccccgggc
     3601 gaaaacgcgc agtctgtggc ggagcttatc gtaggactga ccctcgctct ggcgcggcgg
     3661 gtatatctgg cggaccgcat gctgcaaacc ggcgaagccc tcgccatagc agatgcacgg
     3721 ctgacaggca tggagctgca cggcaaaacg ctgggccttg tgggcgtggg cagcatcgcc
     3781 cgacgtgccg cagagatgtt ccgccgtgcg ttcggcatga gcattgtagg gtattcgcct
     3841 tctctgacag aggaacgcgc ggcatgcctg ggtatagccc gctgcacttg tatacgcgag
     3901 gtatttgccc gggcggacgt tgtgagcatc ggcgtacccc tcacgcctca gacgcgcggg
     3961 cttgtaggta cggcggagct tgccgcggca aagcccggcg cgctgctcgt caatacggcg
     4021 cggggcggcg tggtggacga ggatgcattg taccgcgctc tgacgcaggg tcctctggga
     4081 gctgctgcct gcgatgtgct cgcccacgag ccgcccgcac ccggcctgcc gctgctgcgt
     4141 ttgcccaact ttctggcgac gccgcatctc ggtgcagcca cggacgaagc gctgctgcgt
     4201 gttggcatgt gcgttgtgga gcagatcttc accgtgctgg acggcaaaac gcccgctcat
     4261 gaatgcatcg gcacaacaga ccggacggat gtagaatcag aacacaaatg aaaaaacgga
     4321 tttgatgata acgcgggatt tattgagaaa taagagtgga gggaagaatt tgaatctgat
     4381 ccgaattggc atagaagatg ctcgcaaatt gtggcaaatg cagacagaag catttcagga
     4441 tttatataaa aagtaccagg acacggaaaa cagccctgcg gctgaaaata ttgataaaac
     4501 cattatgagg ttgcagcagc ccttcaccta ttactatttt attcaggaat tcgatgaaaa
     4561 cgtcggcgca gtacgggtcg ttgacaaagg agaaaaagac acagctaaaa gaatatctcc
     4621 tatttttgtg cttcctcaat atcaacggct cggcattgca caaaaagcca tccgtatggt
     4681 tgaagccctg catggcagtt caaattggga attaagtacg attttacaag aaaaggtgaa
     4741 ttgccgtctt tacgaaaaaa tgggttaccg tcagacggga aaaacagaag ttattaccga
     4801 taaaatgacg ctggtatttt acaaaaagga ctgagctgca aaagacagct gaaagcccaa
     4861 gccttaagga gatggttcta aaacggaaat cgccgtcgta cggagcgcca cgttttcggc
     4921 gccgcccttc gcagggaggc cgcagcgcgc ccgacacgcg ctggcacgct gccttcgccc
     4981 tccccgttgc ggcctctgct ttcccgtctt tttccaacaa tacgaatcgg ggcgccctcc
     5041 gcaaggaagg cgccccgatt catacttttt gtgtcgtctt gacaaatacg gcagcgtcaa
     5101 tgac
//
LOCUS       DAWBSB010000161         5082 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_2930_length_5082_cov_3.658047, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000161 DAWBSB010000000
VERSION     DAWBSB010000161.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 5082)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5082)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5082
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2930_length_5082_cov_3.658047"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1062 a         1599 c         1409 g         1012 t
ORIGIN      
        1 cccgatgtgg ctgcaggtgg acgcggaaaa gcgcatctcg tttgaaaacc ccatgcgcgc
       61 cgcagaggcc gatacccgcc tggtgcgtga cacgctgttt actgcgaacc tgcgcggcat
      121 ccaggtggag accgtgtgcg ttttcaccaa taaaaaagcg acccttgcgc tgccgcgcgg
      181 cacgggccac tacacgctga aggacttcaa ggcgctgctg ggcaagagca agtacgagca
      241 ggacaagggc gtcgatattc cccagaccgt ggacgccgtc aagaagtggg tcgtagaggg
      301 ctgaggcccg ccgtcttgtc ctattatagc aaaccgtggc ggatttgaaa agccgggcaa
      361 aactatttac agtttgttca cagcggttta acaaacctta cgcaattctt ttctataata
      421 caatcacagg gacacggaag ccgcaatccg ggttcctgca catgattcct cctcacacca
      481 agagagatac ccctaaaagc ccccgtgctc accacacggg ggctttccct ttgcaaaaag
      541 ctgcgcggtg cgggacgctt ccgttcccgc gcgccgcgca gtttttttct ttcaggtttc
      601 agcgccgcag cccgcctgcg ccacctcggc cgcgatatag cccggcacct gctcaggcgt
      661 gatgcccagc gcggcggcca gctcaccatg aagcagctcc tcggcacgct tcatatattc
      721 ggcctcggta cgtgcgggct ttttgccctg cgccgtacgc tccctgtttt tgccgtagat
      781 cgtcttgata agctgcacca aatcgtcgca gtcgtgcgtg cgcatgagcg aacggtagtg
      841 ctcggccagc atctgctgat ccttcccgcc gcatgggttc tcctcaatct ccgggatccg
      901 gccgatgagc gcctctgcct gggcatgcgt cagaatgggc cgcataaaca ccttggcgtc
      961 cacaggaata tataccgtgc ctgtttcgta cacagggtcc agcttataat acagcttttt
     1021 ttcgccagcg cccggcagcc ccgccggagc ctccacagcc tccacacggc atacgcccat
     1081 attcccgtac acgatcaaat cgcctttttg aaacatcgta ttccctcctg taaaaccact
     1141 ccacccgttt cgtcccgaac aggcggacgc gccgcgccgt gctccctccc ctgcgcttcg
     1201 ctccgccgaa aatcaaaaca gcgtgcggtt ttgacagccg gacttacgtt cctcaaaacc
     1261 acacgctgca atttttcagt atcccgtttt gccgccgctg cggcatttcc tgtttatagt
     1321 ataccacatt ttgcccccaa ttgtaagcgg aagttttttc tctccgccgc gcgccctccg
     1381 aaaccgggcg gacagcctgt ccaaaatttt atccgggccg gacttttccc caccccctat
     1441 gcatataaat tgcatgaggt gatgtcaaaa tgtacaagcg gcgcagaagg cggcatattg
     1501 taatgcccgt gctggcgggc ctgctgacgc tggccacgct gggcagcacg gccttcgccg
     1561 cgccgtcctt ttccctcccg caggctgccg cgtatttcag cagcattctg tgcgcgccga
     1621 atgcagcgct ggccgcaacc cggcgagtgc tggacgacgc catggacgag gacgcagtgc
     1681 ccacggcgca gcctatcgat gaggacgatc ccgaggcagg cttctggacg gtgggcccga
     1741 ccgaggagga aatgctgcct cagagcgaag cgcaaagcgt atcttccggc gcttcctccg
     1801 taccggacgg ccccattccc gatggcatgg ccccgctgct ggaagcgcat tacgagcagg
     1861 gcgcgggtgc gggctatgtt tccagcggcg cagccaccgt gcgcaacagc accgacctgc
     1921 ctgacagcga ggtagccgcg gaaatgatgc agacgcttcc gttccaggtg gagctgaaca
     1981 gcgcggagcc tcaggtactc attatgcaca cgcacaccac agaaacctac gaactggagg
     2041 aaaaaaactg gtgcgatccg gcgttttccg cccgcagcac ggatctttct gtcaatatgg
     2101 ccgcagtagg agccgaaatg gctgctcagc tcaacgcggc gggcgtaaac accctgcacg
     2161 acaccaccct gcacgattac ccaagctaca acggcagcta cgccaaaagc aacgccaccg
     2221 tgcgcagcta tctggagcgg tatcccagca tcaaggtggt gctggacgtc caccgcgacg
     2281 ccatccagcg ggacgacggc acccgtgtca agcccgtggc ggacatcggc ggcatgaagg
     2341 ccgcccaggt catgatcatc tgcggcgcag acgtggacgg caatctgccc aattttaaac
     2401 agaacctccg ctttgccagc cgctggcagg ataagatgga aagcatgttc cccggcctca
     2461 cacggcctgt gctgttcgac taccgctatt acaatcagga tcttacgacc ggcagcctgc
     2521 tcattgagat gggcggccat gccaatactt tggaagaggc caaatactcc ggccgcctgg
     2581 ttggtcaggc cctggctgct ttgtttgcag agggctgaac gcgcccgtat ataaactctt
     2641 cggccacgca aaaagccccg cgcatacgca cggggctttt attattggta cagtcttatt
     2701 ccgccacttc ttcaaaggcc tcttcaccgg cgccgcaaac ggggcacggc acgggaggaa
     2761 cgtcgccctc aaatgtctgg ccgcaaatcg tgcatctcca ttttttcata atactaacct
     2821 ccttttattc atttcgtttc acctattgat attatatacc aattgataat acaacgcaat
     2881 agtttttaaa aaagaatcat tcaccgcgca cggctttggc aaaatcccgg ccgaactgta
     2941 cggctgcagc caggtcctcc tgtgtggggc agaaccggac acgcatgccc tccagcggca
     3001 ggctcagccg aagctgctgc atgcgcgcag tcagattggg aaccgcctcg ccgctccagc
     3061 cgtagctgcc gaacgcaccc gccggcttgc ccttgtggat gatggggttc aggccaagaa
     3121 acatttcgta cactggaggc agcgcgtcgc cgaccagcgt ggggctaccc agcaaaaagc
     3181 cgtccgccgc ggctaccgcg gcctttgcag cggcggtgtc gtcggagacg aggtcgtaca
     3241 tttccacacg cacgccgcct tccttcaagc cgtcgcggat gtgctcggcc aggcggcgcg
     3301 tatagccgta agcggaaacg taggcaactg ccaccacttt ttccggcctc tgttcaggcc
     3361 ggcaccaggt gcggtacatg tcaaaatact tctgcacgcc gctgcgcagc acggggccgt
     3421 ggccgttgcc cacaaactcc acgggcaggc tgccgatctt atccagtgcg ctacgcatga
     3481 acgggtcttt gaacggcccg atgatgtggt cgaaataata tttgtacgcg ggagtgaaat
     3541 cgccgtcgat gaggtcgttg aatacgcgcg ggtcggcata atggcagcca aagctgtcgc
     3601 aggtgaacag ggctttcagc tccgggatgt acgtatacat gctgtcgggc caatgaagca
     3661 tgggcacact gaggaatttc atcgtcagac cgcccaggtc aatctcgtcc ttctccgtga
     3721 cggcgcggga cggaaacggg ccgttgacga tttctttcaa aaacgtcagc gccgttccgc
     3781 tgcccagcac cgtggcgccg ggcgcaagct ccagcagccg cttcatgctg cccgtgtggt
     3841 ccggctcggt atggttgacg acgatataat ccagagaagc cgggtcgcac acctcgcgga
     3901 tattggcaag gaactcctca aagaacggct ccttgctcgt ctcgaacagc accgtttttt
     3961 cagtgccctt cacgatatag gaattgtagg aagtaccgtg gtctgaatgc atgatgatat
     4021 cgaacacgcg caggccggga tcctgcacgc ccacgtacca gatgttttct ttcgcctgta
     4081 aagcgcccat aaaatccaat ctccttttat cggttttgca tcatacattt tatcttattt
     4141 tgttgaaagc cgcaaggacc gggccaggcg cgcacttcta gtatatgcaa aaaatctccg
     4201 gcaaaccgcg gtaccggaga ccttttatct gttccatgcc ggcagccgtt tcacgcatgc
     4261 gcccggcggt agaccagaaa atcaaaatgc agttctgttg taagcgtatc cagcgaggcc
     4321 agcctttcag cgccggagcg gggcgttttc caataataag gcgtcatcgc aaacaggtcc
     4381 aggatcgtgc tgcccgtcac ggtgagcatg ctgtgtacgg gtatcctctg ctccagcgca
     4441 aagccgggat aagcgacatc ctgcaccgta ttttcgtaag ggcgctcgta gagcacctcc
     4501 ttaaggccgt acaggtggcg cgggccgggc accgcaaaca cgaatgctcc gccgggcttc
     4561 accacacggg caaattcctg tgcggcggcc gggctgaaaa tatcgaccag gcaatccgcc
     4621 gccgcatcgg ccacagggat ggcgaagctg ctggccacgg cccactgcac ggcatgtccc
     4681 ttgtcccgcc tggccgctgc ctttaccgcg aatttggaaa tatcgaacgc ggccgccgac
     4741 ggcgttttgc ctgatgcgcg cagcgcctgt acaagacggg cggtataata cccttcccca
     4801 cagcccgcgt ctaccaatga gggagccggc agctccataa gcaggctgca gacaagcgca
     4861 ttcagcccgt cggaaaagca ggcatacccg cccgtatcca gaaaccggcg gcgggccgcc
     4921 accatctccc tgctgtcgcc ggggattttg gcgtgcatct gggcggccgg cagaagatgg
     4981 gcgtagccct cagccgcgag gtcaaaactg tggcgtttgg gacagaccag gacgcgggcg
     5041 ccgcgtgtca gcggcgcccc gcacaccggg cagcggaaaa gc
//
LOCUS       DAWBSB010000162         5060 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_2945_length_5060_cov_2.673327, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000162 DAWBSB010000000
VERSION     DAWBSB010000162.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 5060)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5060)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5060
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2945_length_5060_cov_2.673327"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1660..1669
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3164..3173
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1099 a         1475 c         1534 g          932 t
ORIGIN      
        1 ctcgtatgtc agtcagctga gcagcgaaag cgaccttgaa aatttgaaat tcccggacat
       61 ccgcgtggac gaggcgaagg acaaaaagcg ctgggaaaag gtcagcgaaa tggtgtcggg
      121 gatactgccg gtgcgcctga caggcgtgcg catgttccac tgcggtatct gggacgacct
      181 tgtggaggcg atcgggacag ataacttttt cttctttttt gcggacgagc cggagctgct
      241 gcacaaggcg gccagacgca tggcggacat ttgccagagc ctgatcgacc agctgacgga
      301 aaagcagctg ttcgacgcat atgagccgac ggtacattgt acgggcgcgt atacagatga
      361 gctgccgcag gacaaagaaa aaaacgttcg gccgggagat gtatggacat ttggcttggc
      421 gcagatgctg gggtctgtct cgccccagat gtttgaagag tatgaggtcg aatatgtcaa
      481 gccgctgctg gagcagttcg gcctggtcta ttacggctgc tgtgagccgc tgcacaaccg
      541 gatcgattac atccgcaaga tcaaaaacgt acgcaagatc tccatgagcc cctgggcgga
      601 tatcagagcg ggagcagagc atatccacgg cgactatgtc atctcccgca agccgaaccc
      661 ggcctatctg gcggctgctt cctttgaccc ggagcttgtg cgcagggaat tgcaggagac
      721 ctgccgcgcg gcaaaggaga acggatgtac ctgcgagctg attttgaaag atgtcagcac
      781 ggtgcagtac catcccgagc ggctggcgca gtggcacaag attgcagtag aggttgccgg
      841 tgaatggtaa aacggcggct cataccgtat aaagcggatg cggcgcagcc ggtaaatgct
      901 gcctttggga tttttttgct ttaatgttat tgatgttcca aacgaaaaat tgtatgataa
      961 aggagagaaa agcagcgcac ggggaaaggc gggaacgcag atggaatacg agcaattcga
     1021 ccgcaggctg gcgcgggaat gtgcaaaggc cttttcccaa tccacaggtc tgggctgcac
     1081 gctgtccgac aaaaccggca gggcttttga tgaatacggg tacggctgcg agagctgcgg
     1141 catgtgcgcc gccgcaggcg cctcccatga ccggtgcgtg caggcgcata tctacggtat
     1201 gacggaggcc gagcgcttcg gcggcaagta tatctatttt tgtccgatgg ggctcacatg
     1261 ctttgtctct ccgattctgg gcgagggcgg cgccgaggcg aaaatcacgg tcggcccgtt
     1321 cattatggta gaaaaacagg attttatcgc ctgcgagctg acggaaaatg tccgcctcac
     1381 agaggcacag aaacaggccg cggtgcaggt gctggagaat attccctttg tgccgacaga
     1441 acgggtgacg cagctttcgg tgcttttgtt catggccgtt gggtttatga acaatgtttc
     1501 ggcggaaaac agaatgatgg agtcgggcca ttcagaggag ctgcaggggc agatcacttc
     1561 ctatatcctg gagcttaagc aagaggaagc gccgccgccc tatccgtttg aaaaggagca
     1621 tgtgctgctg cagtgcgtgg cccggaagga ccgcgacggn nnnnnnnnna tttcccggac
     1681 agccatcgag aacggcgcgg acgcagagca cacgatgcgg ctgagtcatg aataccgtta
     1741 caggatcggc gcattcacca ccattgattc gctctgcctg tggcttgcag gcgtggtgaa
     1801 ccactttatg gacgacctgt tccgcttctc ggacgccaag cacgccaaca tcatccaccg
     1861 gtgtacacag tacatcagcg tcaattacaa agagcgcatc acgctggagg acaccgcccg
     1921 gatggtatat ctttctccgg cttacctgag ccgcattttc aagcaggaga cgggcgtgac
     1981 gttcaacgag tacctcaacc gcgtgcgtgt gaacaaggcg aaggaacttt tgcgccgccg
     2041 cgagctgcgc atgacggata tttccctggc tgtggggtat gaggatcaga gttatttcac
     2101 aaaggtgttc aaacgtgtgg cggggatgct gccgcgggaa taccgggaaa aaatactggt
     2161 ctcccggaag gactgacgga aggaacaaaa agcagacgga gctgaaagct ccgtctgctt
     2221 tttatataat acagtttatg cctgcggccc aagctgcttt gcacgctgca ggcaatagcg
     2281 cagcggctta taaagcagca gcgccacaac aaaattgccc gcgccgtgca gcaggtcgaa
     2341 tgggatgcct gttacccacc aggcaacgcc cgcggcaatg ccgccgttca tcaccgcgta
     2401 ggaaacagcg tacagcgcgc cgtagcaaag accaaacagc ccgctcagca gggcccagag
     2461 cagcacagag cggttttgac gcatcagccg ggcgaacagc atcagcaccg cccagatata
     2521 cagctgcggg aaccaccaga tgccaaagcc gttgagcagg ccgtacacaa agacataggt
     2581 ataaacggcg ggcacggcca gccgcggata ttccagcgtg tacaggatga ccagcaggct
     2641 caccagctcg atattgggca ggccgtccag cgcgaatttg gaagccacca gcaccgccgc
     2701 gaggaacgcg gcggtaacaa ggctgcgcgt cttgcccatc tcagtagccg gttttcagcg
     2761 tgatctcaaa gtggtcgccg tccgcgaccg gggtggagtc aacgccggtc atcacatcct
     2821 cgccgtcctt cgtgaagcac caccactcct ctttggcctc gtcggccgtc acgccgttga
     2881 ctgtttttac atacagaccg tattgggatt cttcgccttc gacaagcttc tgctcctcca
     2941 gtgcctggcg cagaaattcc gcgtcggtag agaagctgaa ttcatcggtg gtcgcgtcgg
     3001 cgtatacgac ggttacgccg atggccttct gcccggcggt acccttcggg ccaaaggcaa
     3061 accataccgc cagcattgcg gccacaagaa cgaccgcggc gcagacgatg atcagcgttt
     3121 ttttcttttg tgcagacatg tttcaattcc tcctgaaagc aaannnnnnn nnnaaataaa
     3181 aagccgcgcc tcccggaccg ggagcgcggc ttttttgaca aaacagacgc gcacgatcca
     3241 tccatcgtaa atcgtgagaa cagcgccgtg cccggtaccc cggctgaggg caaagcccaa
     3301 tacggtggcg ggaccgcgcg ggagtttcac ccgcttcccc ggaaatgcgc ggcgcaatat
     3361 gcaattgtat atgctacagt gtacaggaca ggcacgtttt ttgcaagccc ctttgggagc
     3421 gtcagaaaaa cataccttcg gtataggcgt tcatgaattc cgcgctggtg gaaaggtcca
     3481 ccgtcctcgc tccggcagcc agggcctcgc tgtgggggat aaagcccctg ttcagcaaaa
     3541 gcatggccgc gccgctcaag gccgcgtttc ccagcacgcg cacacgggga acgagttctt
     3601 cgggcagaag gccgatgcgg cccgcgctgt ctacatccag atagctgccg aagccgcccg
     3661 cgacggccag ctcggcggcg tccgccccgc aaagcccttc cacgcgcagc agcgtgcgaa
     3721 gcccggcgca gatggcgctt ttggcaagct gtaccatgcg gacgtctttc tgcgtcaggc
     3781 atacaggcgg agcaacgggc gcagggtcct gctccagaag cccggtttcg tccagctgct
     3841 ccagctccac caggcaggca agcgcgtcga tgacgccgct gccgcagatc cctttcggcg
     3901 cgccgccccc aatgacgtgg gcctgcagtg cgccgtcctg tacgcggacg tgatccactg
     3961 cgcccgtctt tccggccatc cccatggaaa gcccggcccc ctcaaaggcg ggcccggcgg
     4021 ccgtggagca gcaggaaagc tgcccgcgat gccaaagagc gatctcgccg ttggttccaa
     4081 tatccaccag catgcgggtg tccggcttgg agcagatgcc gctggccagc aacgccgtgg
     4141 tgatgtccgc acccacaaaa gcggacatac agcggggcag atatacctgc gcatgggggc
     4201 agggcaggcc aagctcccca gccgcaagcg tttctccaaa caggcgggaa gcggcgaacg
     4261 gggcatggga aagacagtcc gggtctgtct gcgtgagcag gtacagcatc gcggtgttcc
     4321 ctgtgatgac cagcgcgtcc actgcttcag cggagatatg cgctgaagcg gccatttcca
     4381 acaccagtgc ccgcacaccg gcgcgcacgg aggcggccag cgcatcgccg ctgccgtgca
     4441 gtgcggcctc gatgcgggag atgacgtcgg cgccccagcc cgcctgcgga ttcggcgcgc
     4501 tggcctgggc cagcagcgta ccgtccggcc cgtacaggca ggacgccagc gtagtcgtgc
     4561 cgatatccac tgcggctccg cagacggaaa agattgggtc atgcacaaaa tccggcatct
     4621 cgccggccaa acggatctgg ctggcggcag caccctcaag cgttaccgta cagtcgccct
     4681 ccacacgggc acagcaggcc agacgcacgc cccgggagat atcctgcggg gaaaggtgtt
     4741 cccgctctgc gtctgaaagg gcgctcagcg cgccgtgggc gacgacgcgg cacttgccgc
     4801 agcggccgta tccggcgcag ggcatttcca gtgcatggtg cggcagaatg acgctgctca
     4861 gcagcgcacc catctgcgct tcaaggtgtg ttccgttgac gctcactttt gccaaagcgt
     4921 tccctcccac agttgactgc cgcccgccgg cgctcatgcc tcgcagaaca gcagcgcgcc
     4981 gaaataagtt acggtgttct gcccgttgat gtacttcatg ccggccagct ccgccagttt
     5041 ggacacgttc atgccatatg
//
LOCUS       DAWBSB010000163         5020 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_2971_length_5020_cov_2.485599, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000163 DAWBSB010000000
VERSION     DAWBSB010000163.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 5020)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5020)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5020
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2971_length_5020_cov_2.485599"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1105 a         1495 c         1460 g          960 t
ORIGIN      
        1 cgcgcgccat taccccgcca tcaactggac catgagctac agcgaatatt acgaggacct
       61 gggcccctat tacacggcga acctgggcga ggagttctgc gcgctgcgcg ggcgcatgct
      121 gggccatctg agcgaggaaa acaagctgat ggagatcgtc aagctcatcg gctcggatgt
      181 gctgcccgac agccagaagg tcgtcattga gacggcgcgc gtcatccgga cgggctttct
      241 gcagcagaac gctttccaca aaaacgacac ctatgtgccg ctggacaaac agatgtggat
      301 gatggaggcc attctccacc tgtatgaccg cgccgtggag ctggtggaca agggcgttcc
      361 cgtcagtgcc atggccgaag cggggctgtt cgacaagctg gtaaaggtaa agtatgagat
      421 tcccaacgac aagccggaaa tgttcggcga gtacaacaag gagctggacg atatgtgcgc
      481 gcagctgctt gcaaagcaga cccctgcggc gggctgaacg gaaaggagcg tttgcatatg
      541 gcaatggaat atttgggcct ttctgacatc aacggcccgc ttgtcgcggt ggacggcgta
      601 aaaggcgtcg gcttcgagga aatggtggag atgaaaaccg ccaccggcac gcgcctgggc
      661 cgcatcgcgg cgttggaagg cgagcgcgcc gtcatccagg tgtttgcggg caccagcggc
      721 atgacgcttt caggcacggc cagccgcttt acgggcagcc ccatgcgcct ggcgctcgcg
      781 cccgagatcg tgggccgcat tttcaacggc gcgggcgaac ccatcgacgg cctcggccct
      841 gtttacgccg agcgcttcga ggatatcaac ggcagcgcca tcaaccctgt ctcccgcagt
      901 tatccgcgca actacatccg cacgggtatt tcttccatcg acagccttat tacgctgatc
      961 cgcggccaga agctgcccat cttctccggc agcggcatga gccacaacaa gcttgccgcg
     1021 cagatcgtgc ggcaggccaa gctgagtgag gatgagggtg ccaacttcgg cgtggtgttc
     1081 tgcgccatgg gcgtgaccaa ggacgtggcg gaatatttcc gccgcagctt tgaagaatcc
     1141 ggcgcgctgt cccgtgttac aatgtttttg aacctctcgt ccgaccccat cgtagagcgc
     1201 atcctcacgc cgcgctgcgc gctgacagcg gccgaatacc tcgccttcga gcagggcatg
     1261 catatcctcg tggtgatgac ggatatgacc agctacgccg aagcgctgcg cgaattctca
     1321 tcctccaagg gcgagatccc gggccgcaag ggctatccgg gctatcttta ttccgacctc
     1381 gccagcctgt acgagcgcgc gggcgttatc gaggggcgcg agggcagcgt aacgcagatt
     1441 cccattctga cgatgcccaa cgacgatatc acacacccca tccccgattt gacaggctat
     1501 atcaccgaag ggcagatcgt gctggaccgc tacctggacc agcagggcgt ttatccgccc
     1561 atcagtgttc tgcccagcct gtcccgcctg atgaaggacg gcatcggcga aggctacacc
     1621 cgtaccgatc acgcggacgt ttccaaccag ctgttcgcca gctacgccaa ggtaaacgac
     1681 gcgcgggacc tggccagcgt catcggcgaa gaggagcttg gcgagaccga taaactgtat
     1741 ctggatttcg gcgcccactt cgaaaaggaa tttctgggcc agggccccaa tgaggaccgc
     1801 accatcgacc agagcctgga ccttggctgg cgcctgctct ccatcctgcc ccgcgaggag
     1861 ctggaccgtg tggacgaagc cgtgctggaa cagcattacc gcaagcattg agccggccgc
     1921 ccatgcgggc cggacagatt cattgaaatg gaggtgagcc catggccgaa cagatcttcc
     1981 ccacaaaggg caatttgatc gcctgcaaaa aaaacctgac gctggcaaaa ctgggcttcg
     2041 accttttgga ccgcaaacgc aatgtgctga tgcgcgagat gatgaagctc atcgacgaag
     2101 cggccggtat gcagcgcgcc atcggcgaca cgttcatcaa ggcatacaaa gcgctggaga
     2161 gcgccaatat cacgctgggc atcgagcatg ccctggaggg cgccgttccc gaggaaaaag
     2221 gcttttccgt cacgggacac agcatcatgg gcgtggacct gcccaccggg catctggcca
     2281 gccggcctct gtacccctac tacggcttct ccggcagcaa tgcccagctc gactacgcct
     2341 atgtctgctt cgataaagtc aaacagctct gtgcgcagct cgcaggcatt gaaaacagcg
     2401 tttaccggtt ggccatggcc atcaaaaaga cacagcgccg cgccaacatg ctggaaaatg
     2461 tggtcattcc ccgcctggag ggcaacatca aatttatttc cggtacgctg gaagagcatg
     2521 aacgcgaaga attcacgcgt ttgaaggtca tcaaacggat gaaggtaaaa aaatagcctt
     2581 atgctttttc gtgtccgcag ccttttcccg gccgcggccg tcacagacag aaaaaacgac
     2641 cgtttcctgc aaaagccatc gcccccggcg gcgcatcccg cgcctaccgg gggcgatggc
     2701 tttcataacc tgtggaacag acgtttgagc attctgccca gaaaaatttg aatgatttgt
     2761 gaactttccc gattcccgct tgacgacagg tatattttgc cgtatattag ttaagcaacc
     2821 taattattaa aattgttaac aattaggcat attaactttt aacaagcaaa tccaacagga
     2881 ggtaaaccat gaaggggctg gaaaacaacg ccgccgagct gttccggctg atggaccggg
     2941 tgcgcaaagc ctggcagagc gtcaccccgt gcgaacatct gagcaaatct cagtttggca
     3001 cacttatggc cattgcccac cacggcagaa tgcccgggca aagctgcacg ccgtctcagg
     3061 aacatggcgc tcttacgctg accgcgcttg cgggcgccat gcatcaaagc ctgcccgcat
     3121 tgagccagcg cgtcagcgcg ctggaggcac agggctatgt ggagcgcgtg cccgacccgg
     3181 atgaccggcg tgtcatcggc gtctgcgtca ccccggaggg gcgcactgtg atggaagtgg
     3241 cctaccgccg tttcggcagc attctttccc agtcgatcga ctatcttggc ccccaaaatt
     3301 ttgaaacgct tttgcatctg ctgggcgaac tggccattgc gctggagcag gcggtcgccg
     3361 cacaggaaga agaacgggag agtgattcca aatgatgaaa ttgaagcgct acctaaagcc
     3421 ctatatcggc ttcgcgctgg cggccatcgt gctgatgttc tgccaggccg ccgtggatct
     3481 taatttgccc aacctcatga gcgatatcgt gaatgtgggc atccagcagg gcggtatcac
     3541 cgaggccgcg ccaaaagcgg tttccgccga gggcttgcag ctgatgagcg cctttatgtc
     3601 cgaggaggac cgggctcttg cccaaagcca gtatgtcctg gccgaacagg caaacatgac
     3661 ggcggatgaa tgggatcggt ttgtgacaga ttggcccaag gcatctgaca ccgatgtata
     3721 ccgacagatt ccgaatctgg acgacagcac gcaagctcaa ctggacagcg cgttcagccg
     3781 tgcgtcctat gcgctggtga ccgttgttaa ggaccttgcc gagcaaagcg ggcaggacat
     3841 cgctgccggc gacaccgaca gcaaggccat ggacatgtcc aaactgggcg agcttgtccc
     3901 catgcttgcc atgctgcctc agggcatggt agacgatgcc attgccgctg ccgccgctac
     3961 gccggaaagc atgctcagcc agacggccgc cgtgttcacc aaaggctttt acatgcagct
     4021 tggcgcggat acagacgcca tgcagacaag ctatatcttc cgcaccggcg gcaaaatgct
     4081 gctgatggcc cttctgctgg ccgtgtgcgc catcagcgtc ggcttctgcg gcagccgcat
     4141 gggcgcgggc atcgcgcggg atctgcggcg ggatattttc gcaaaagtga ccagctttac
     4201 caacaatgaa tacgacaaat tcggcaccgc cagcctcatc acccgcagca ccaacgacgt
     4261 gacgcaggtg caggggcttg taacgatggg cctgcgtatc atgtgctacg cgcccatcat
     4321 cggaatcggc ggcaccatta tggccctgag caaaagcgtg agcatggcgt gggtcatcgc
     4381 gctggccgta gtgctcattc tgggcctcat ctgcacgatc tccgccatcg ccatgccccg
     4441 cttcaagatc atgcagcagc tggtggataa gctgaacctt gtcagccgcg agaatctttc
     4501 aggcatgatg gtcatccgcg cgttcgccac gcaggatttt gaagagaaac gcttcgacaa
     4561 agccaataag gaccttgccg acaacaacct gttcgtgttc cgcgccatgg ccacgatgat
     4621 gcctgtgatg atgctggtaa tgaacggcgt ttctctgctg atcgtatggg tcggcggcca
     4681 ccagatcgcg gattccgcca tgcaggtggg cgacatgatg gccttcatcc agtacaccat
     4741 gcagatcatt atgagcttcc tcatgatcag catgatgttc gtgatggtcc cccgcgcctc
     4801 cgtctcggcg ggccgcatcg cggatgtcct tgccagcgaa agcagcgtgc aggacgcgcc
     4861 ccagcccagg cacctgccgg aaaaagtgaa agggcttgtg cagttcaacg acgtctcctt
     4921 ccattatgag ggcgcggacg aaaatgtgct ggagcatgta acacttacgg cccggcccgg
     4981 cgagacaacg gccttcatcg gctccaccag cagcggcaaa
//
LOCUS       DAWBSB010000164         5000 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_2984_length_5000_cov_2.388271, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000164 DAWBSB010000000
VERSION     DAWBSB010000164.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 5000)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5000)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5000
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2984_length_5000_cov_2.388271"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          919 a         1546 c         1358 g         1177 t
ORIGIN      
        1 agtgtgtagc gccggattca acttctaaca tcaaatagta attcccattt ggggagttta
       61 tattgaattt agtgcttgcg gtatatgttg tgtcgaatcc gtttgcttcg gaataaataa
      121 gctgttgaac aacattcccg tttatatcac tcaatgccac cttgatatgg gaaaagctat
      181 ttgtgataaa tgcccggaga ttggcaaaat atatttgtga ataacctatt gtgctaaatc
      241 caccaagcat tacacggcca tacccgcgtt ctgttcctcc gaatgcgtca accttaatat
      301 ctgaaccact atttgtcacg gtaaaatatg cggcatatcc aaggttctgt gtattcgcaa
      361 ctggtacgcc gggatagata gttttctgct tttgccaaac aaggttgtta ttgtgataaa
      421 ccttgttgct ggcattactg ttgtaataca cattgttcga ctgtgggacg ttgttgctgt
      481 tgtaatatag cgccatgtct gcaccgcctg ttcacgaaaa tgatatccat aggtttccat
      541 cataactcat accgatggcg ttgttattca ctttggaaag ccccacctgg tctttggtgt
      601 gactgtgtat gctgttggcc ttgccgttca gtttggtgtt catttccgtt tcggtgtaat
      661 atcggtcgtc gtgggtgtgg cttgccttcg cctgttctac gggttggttc gattcgttgc
      721 cggcatagac ggtcccgtcc ttggcgacag cgatctcgct gtatggcaga tcagcggggg
      781 cttttgtggc gccgctctgc cggtaatgtt tgatacttcc ttgtttggcc atcagtatgt
      841 ccctccatcc aatttcaaaa tcggcacgcc gtcgtcatcc agttccagca acagatttgc
      901 agccagcgcg ccccaggaat cgccctgctg ttggacggta ctggatactt ccacggcaac
      961 atcctccagc acagtcgcgc cttgccggtt gcggaacgta agccggggct ttccgtccgc
     1021 ctcctgcaca atcatgtctt taaatgcacg cgccaaatat gccacctccc atggtaaaag
     1081 ccaatgtctg ccttgcgttc cgctgtgccg tcagagctgt gtacagcccg gccagcgcgc
     1141 tttctattcg gttcaggtcg tcatagtccc atacacggcc attggcctca taggcgcgcg
     1201 cccgctccga gcggggcgga cggaacgtgt tgtccagcag cgcgtccaca ttgttgtcta
     1261 cgttggcgaa gaactccaca tacggaaatc ctgccgggcc atagtcttcc atatccgcca
     1321 gcgggaacgg cagatacaac tgccgggcca tgtcccgcag atgcagaata ttgccgcgaa
     1381 tgcgggcata gtccgggtcg agcatgacgg ggtccgtttt cttccagtcg gttttagggg
     1441 ttacccatgc catcaatatg tccctccatc cacgctgccc gccttatcca ccacgccgtc
     1501 accgtccgcg tcataggtgg aaaaccgctt cttgattttg ccaaacagca cggacagctt
     1561 ttccccgctg gcaatatccg cgtcctgcgc cgtctcggta aattccaccg ttacattgga
     1621 gccgttcccg gtctccggta ataacggcag cccccggccg tccgggtcat atacagcctt
     1681 tgtcatgtcg ccgctgccgg aagccgccat ctggtcatcc acatatttct ttgtagtcgc
     1741 atgcatattt tccgtgggcg cggcatgcag cgttaaatcc cccgtcatca cgccgccaga
     1801 aagaggcagc gcccccgcct gctgtgcggt cacaccatgt gggttgctcc cgctgtccat
     1861 gtgcgccttg gccgctgcca gcgtggtggc gggcgcgccg ttccaggtgc cggtccccgc
     1921 aaccgccctg atcgcgtttc cgatggccgt cagcagcgtc tgcagcgtac cactggtcgt
     1981 cagtaccgtg cgcggtccga ttttggcgtc cgtaacgctg tcgtccgggt ggtccagtac
     2041 gtcagcggct ttatgctgcg acaattccag ctttgtggcc ttttcctttt ccagccgcac
     2101 atcctccagg cgctggcgct ccatctcagc gtctatgatg ccgaggtcgt aggtcaggtc
     2161 ctcgatgtcg gccgcatcgt tcgcctcgcc ctcccgggcg ccgcgctccg gcaggcgcat
     2221 gttgtagaat ttgctgcgtc tcattcttcg tccaccatcc ttttggcgat catctttcct
     2281 ttcagcccgc cgctgaacgt cagctcattt ttgagcaccc gcgcgggcgc ggaaaacggc
     2341 gcgaactggc tttccagcca gattaggtcc ccggggtcca gctccgggct gccccggtac
     2401 tcaaattcgt aggtattccg caaaagcagc cagtcccgca cccattcggc caccgccagc
     2461 gcgtgcacgg cgtcggtgac gagcgggttg tccagcgtct ccacgctccc gttatcatcc
     2521 ggtttttcca cgggcgccgt cacggtgcgg gcgctgcttt ccagcttgcg ccccgtcacc
     2581 gttaccacag cttcgccgct cccggtaagc gtcaaatccg ccgcagccgc aaaaacggcc
     2641 tttgcggcca ccacagcccc cgtcacatcc actgccacgt ccgccgcctg cgaaaaggtc
     2701 aggtgcagct ccagcgtgcc atttacggca tagctttctt tgtgcagctc gctggccgct
     2761 gcctccggcg cgtaagtata tgccgggcac tccacggccc acagcgtggg gatctggctc
     2821 accttggggc gggccagcat cgtgtggaag tccaatttaa aattgtcctg tacgtcgttg
     2881 gcaggttcga tgcggatata cccctcccgg tcggtgtaca gcacgcagca cgccgcgtgg
     2941 gcgatgagct gcagcagttc cttgtgcttt tttgcgggca gtggcgcggt ggtcataaag
     3001 tccgcaagcc cctcccacag cttccagggc ggcgcggcgc cgctgaacgg tgcaaggccg
     3061 ctgtcctcca gcacatccag cgcaagttgg tacagggtgc gcccggccgg agcatacacc
     3121 cctttgtagt aagtgccgtc cagccccgaa agcgcgtcct gcgccttaaa gctggctgac
     3181 agcccgtcca ccgtgggctg gcccgtcagg tagtaccgcc cgccgggcac ccattccgtc
     3241 acgccgccct cgtacacttc gcgccagccg ttcagctcca gctccgccca agtgttcgcc
     3301 gcaacgtctt tccacgtcat gcccccggca aggtactggc cgtactccac cttcaccggg
     3361 ttctgttcgg agatgtactt gtagatgccg gaggcgttgt ccgggtcgta gagatattgg
     3421 cctcccgtgc ccgtcagcgt gttgatgttg acgatcgtaa aatcaaacgt attcgtgggc
     3481 aggcggcggg aaatggggtc cacatccagc ttgtgcacag ccttttccac aaggcggttg
     3541 tcgaacacaa gaccgaagcc gaacatcagc tgctgcagcc gcatgcggcg gtacggcctt
     3601 gtcatgcgcg ggaatgtgaa cgccagcgcg tcaaagcgct cgatggcgtc gggcgtggtg
     3661 tatacggccg cgtccggcgt gtaatccgcc gtgaagaaca cagcgccacc gcggctggcc
     3721 gtcaggtgca tttcctcggg ccagtccccg caggccgggt caaatgtgaa tgttaaaccg
     3781 ggcacggtgt gcgttttgga aaacgtcagc accatgcccg gaacgtccgc cgctgcgaaa
     3841 acaccgtccg caccgcacag tgccgcagac acaaaccccc cgggccgcag gtcggcggag
     3901 ccgggcttgg gggggatcaa ctgcgagccg tcggcaagca tgcgtcccgg ctcaaaggtg
     3961 gcgtaagtgg cgcgcggcgc gccgtcctcc agcagcatcg tgtccgggcg cgagtagaac
     4021 gcccccggcg tcgtttccgg cggcgcacag gtcgcggccg cgtcggtgtc cgtcaggccg
     4081 aaggtgatgc ggatgtatcc ctgcggcgcg atctgctcct gcaccgccgc ctggtacaac
     4141 gatgatgtct tttgcaatca caccaccccg cagtcaatga cgttgaacga cgcgtcgcgg
     4201 taaaatcgcg gttcacctgt ttccgtgctc accacgaaag gatttgtttt cacgtcgccc
     4261 aaataaaaaa gccgcgtttc ccagcggcca aagttgtggt tgaagtagtg gcagtagaaa
     4321 gtaaaatgcc cttgttcaaa ccagcggttc atttcccacc atttttctgc cggcatgacg
     4381 gaccaggcca gactttgctt gtggacgctg cgaccgacca tccggcccac tacctcaccg
     4441 tttgcattgc gagcactgtc caccatgcgt gaggtttcaa aaggtgcctt gccctcatcc
     4501 gggtaaggca ccataatcgc ataattctca cgatccgtcg ctggtgatga gccaagatag
     4561 atatatcctg ttttttcttc catcttgtca atcgcctctt tcctagtatg cttctgcgaa
     4621 tacgccgccc acagacgcgc cgcgattcgt tttgatgcgg tgctggttgc gataaagcac
     4681 atccccgtct atctcttcag tgacgttcac aacgatgtcg ccgccaaatc ccgctgtatt
     4741 ggcggaatcc atcgcctcca gaaaagcctg tttcatccgg tcttccggcg cgacgatctc
     4801 cggattattg ttcgcccccg gatattcgcc gcgtgcgaag agcgtcggtt ccttcagaac
     4861 tgcgccggta gccagcaatg ggatctgctg cgcggtaatg ggagtaatgg aaaatccgat
     4921 gctttttccg ccgaatagcg gaacccaatc cgggatatgc acattcaggt ggttgagcac
     4981 atctatcact gcgttcatcc
//
LOCUS       DAWBSB010000165         4961 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_3010_length_4961_cov_3.348349, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000165 DAWBSB010000000
VERSION     DAWBSB010000165.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 4961)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4961)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4961
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3010_length_4961_cov_3.348349"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1002 a         1367 c         1626 g          966 t
ORIGIN      
        1 gtccaaagat ttgtccgccg aatgcgacag gctggaacgc tcggcggcag aaggcaggaa
       61 aaaggtggaa gaactggaag ctcaggccga aacggacggc ggctgcagag agcgggccgg
      121 acggtacatg aagtccgggc ggctcagccg cgagatagtg gaagcaatga tcgatcatat
      181 ttatgtgggc aagcgtatcc cgggcacaaa gaatgttccc gtggagatcc actgggattt
      241 ctgaatttta tcccccgtcg aggcttacgg agcccagacg cggcaaaagc cttaaagctg
      301 ttcttaccgc atcgcagcag agcagaaagc ccgcaccacc tatgacaata ttctgcgtct
      361 ggtggacgac ccggacgtga tcgcgcccat ccgttttctg cgtgaacggg agatcgtcca
      421 tttccagcgc ttcggcgagg cgctggaaac cgtcaaggac accctggaca gccgcaattt
      481 ttatgccgtc aatcccagct ttgaccgctg accagccaaa agatgcgcgg aggccgcaag
      541 gcctccgcgc ttgctttttg tccgcgagcg ctttacaata aaatcataac gccggactgc
      601 gcggcaggca cggcgaaagc atcacaaaaa agggggacga cccatgaaag cattgttcat
      661 cggcggcacg ggggtcatca gcacagccgt cggcgctctg gcgctggccc gcggctggga
      721 actgacgctg ctcaaccgcg gcaccaacgc ggcccgcgcg ccccggggcg cgcgggtgct
      781 tacagcagac atccatgacg aagctgcggt gaaggccgca ctgggagacg agcggttcga
      841 cgtggcggtg gatttcatcg cctttaccga ggaggacgtc cagcgtgacg tgcgcctgtt
      901 tgcggggcgc gcggcccagt acatttttat cagcagcgcg tcggcatacc agaagcccgt
      961 ggccgcgctg cccatcaccg aaagcacccc cctggcaaac ccttactggc agtattcccg
     1021 cgataagatc gcctgcgaaa atttcctgat ggagcggtac cgggaggaag gctttccggt
     1081 gaccatcgtg cgccccagcc acacctattg cggcggcaag gccgtggtgg cgctgcacgg
     1141 cgcacgcggc tgctggcaga cgctggcccg catccgggcg ggcaaacccg ttatcatccc
     1201 cggcgacggc acgtcgctgt ggacggccac ccatgccgat gattttgccg ttggttttgt
     1261 gggcctgatg ggcaaccccc acgcgctggg cactgcggta cacatcacca ccgacgaggg
     1321 gatgacgtgg aaccagatct acgcggtgct cgcgtcagcc atgggtgcgc cgctgtgcgc
     1381 ggcatatgtc gccagcaaat tcctggccgt atgcggccgg gccgcgggct atgattttga
     1441 aggtccgctg ctgggcgaca aggccgccaa cgtacgcttt gacaacacca agatcaagcg
     1501 gctcgtgccg gatttcgcgc cccgcgtttc catggcccag ggtatccgcg gcgcggtgga
     1561 atatttgctg gcgcacccgg aaatgcaaac gccggacccg gagttcgaca cctggtgtga
     1621 cagagtcctg gcggcgcaga gcgcggccaa agccgtattt gacggcgcgc atctcgctta
     1681 acgcagaacg aaaacacgcc ccgcacggct gaaagccggg cggggcgcgg ccacttgtgg
     1741 cggaaattga gcaaaaatcg caaggctcgg cggaaaatgc cttgcaatta caatggaaaa
     1801 cccgtgcccg cctcgctata ctgaaaagca tacgggcgcg gcgcgggccg cgccggggag
     1861 ggcggatgca tgattcggga catgacgcgc ggcaatgtga cgcgccatct ggtgggcttt
     1921 tccgtaccca tcattctggg caatctgttc cagcttgcct acaatgcggt ggactccatc
     1981 gtcgtggggc gctttgccgg caccggcgcc ctggcggccg tgggtacggc caaccccgta
     2041 atgaacatca tcatcctggg cgtttcgggg ctgtgcatcg gcgcatcggt gctgatgagc
     2101 gaatatttcg gcgcgcggga tacagagaag ctgcggcggg aatttgcggc cacgctgttt
     2161 ctgggactgt ttttttcggc ggcggtgctg gcgctgggct tgccgctggc cgggccgctg
     2221 ctgcgcctgc tgaacgttcc ggacgagata tttccgcagg cccgggcata tttgtgcatc
     2281 atttttctgg gtatgccgtt tacctttgtc tacaacgctt acgcggcggc catgcgcagt
     2341 gtgggcgatt cccgcacgcc catccgtttt ctggcgctgg catcggtgct caacgccggg
     2401 ttggacgtgg cgtttgtggc aggcctgcac tggggtgcgg tgggcgcggg aattgccacc
     2461 gtcctcgccg aaagcctttc tgcaatttta tgtgttattt atacagagcg taatcttgct
     2521 gcgctgcgcc ttaccagagc agagctgcgg ccggacagga tgttgatgaa gctgacgctg
     2581 cagcagggca gcgtaacggc gctgcagcag gcctgccagc cggtgggaaa actgctgatc
     2641 cagggctgtg tgaacgggct cggcgtagag gccatcgcgg tgttcaacgc cgtcagccgg
     2701 gtggatgatt tcgcgtttac gccggagcaa agcatttcct cggggatgat gacatttgtc
     2761 tcccaaaacc ggggcgcgaa ggaatacggg cgtatgcgca tgggcctgtc ccgggggctg
     2821 atggtggaga ccggatattg gatgcttttg ttctgcgtcg tcctgccgct gcgcgcgcct
     2881 atcatgcgcc tgttcgtggg ggcggatgaa agcgccgtgg cactgggtgc gtcctatctg
     2941 ttcctcatgg cgtttttcta tttgctgcct gcgtacacca acggcctgca ggggtatttt
     3001 cgcgggttgg gagatatgaa gatcacgctg gcaggcacgt tcacgcagat atcggtgcgc
     3061 gttgtgttcg tgtatctgtt ggtgccccgc atggggctta cagccgtggc gtgggccagc
     3121 cttgcgggat ggctctgtat gctgctgctg gaggtgccgt acctgcgcag catgcgcgtg
     3181 cggggcgtgg gatgaacggc accggttttc tgcgtcaatg cgctgaaaag gaagctttta
     3241 ataagctgag gcagacactt ttgtgtttgc cttatttttt atcgtttcag ccaacggaaa
     3301 cggaaaacaa aattccggca cgtccgctgc ggaaagacga cgcggcaaaa cggagcagca
     3361 aaaaaatttt ttcggaaagc cttgaccttg tcactgtggc aaggtatata gtcgttacag
     3421 gaccggtcca tacgtgcccg ttcccgggaa cgggcggaag aggagaaaaa cggcatgtat
     3481 accatcggcg aattttccaa tctggcgcat gtttcggcac gcagcctgcg ctattatgat
     3541 gcaatggggc tgctgcgccc cgcgcatatg ggtgaaaacg gataccgcta ttatacaggc
     3601 ggccaattgc cggcgctggc aaagatcgag acgctcaaga gttatggctt tacactggca
     3661 gaagtgaagg agctgctttt gctgccggaa acagaactgg cacaccgcat ccatgtgcgg
     3721 cgcataagcg cgtatgccca attgaacgag atgcgccggg tcatccgccg gatggaagct
     3781 gccatcgttc aaatggaggg aaccagtatg gcaaacgata cttatcatgt aatcttaatg
     3841 caggctccgg cgcagaaggt attcagtgtc cggcggacca ttcagattgg agagatgcac
     3901 gacctgttcg cggaactgcg cgcgcaaatg aagcgggcag gtgtgcggcg taccggcccc
     3961 acacaggcgc ggtaccacgg agaggagttt tcttatgagc atatggatgt tgaagcacag
     4021 gcggctgtag acgcggacgg accgaatgtg tcggtgatcc cggcgggcac gttcgcggct
     4081 gtgacgcata ccggcccata tgagaatatc aagtatgcat atgatgccct gtgtgggtgg
     4141 gtggcgcatc atccggaata taaagtctgc ggtccggcca ttgaacggta tatcaaggac
     4201 gaggccatgg tgcacgaccc ggaggaactg gagacgggcg tgctgttccc cgtttgccgg
     4261 gcagaggaat gaacggttct tgtacagtgc cgcagcgcgc agtataataa aacagcagag
     4321 ataaaaacac agcgcggtcg gtagtccgcg cgcggggtgc atgccctgtc agtaaccttc
     4381 cctcccgggt cgtccgttcc ctgcaaggat tttctaaagg gaggaagaga cggatgcaga
     4441 agtctcgggc gtcgtttacc gtgttttttg aggacccttt ctgggtaggc gtgtacgagc
     4501 gggaggaacc gggcggcctt acggcctgcc gtgtggtgtt tggggcggag ccgaaagacg
     4561 gcgagatcta tgagtggatg ctgcaaaact gggcgcagct gcactttggc cccccgctgg
     4621 cggatgcccg gaggccccgg gcggaaaacc cgaagcgtgc ccggcgcatg gcccggcgtt
     4681 cccttgccgt acaaggcgtg ggaacgaagg cgcagcaggc gctggcactg gcgcgcggtg
     4741 ccggcaaaca ggcccgtgcc gccgcgcgcc gggaacagga gcaggcggac cgggagcggc
     4801 gctttgccct gcgcgttcag cgcaggaagg aaaagcacag gggcaggtaa agacctgccc
     4861 ctgtgctttt cagactgtcg aaaaaaataa agaagtaaaa gaaaagtccg ggggaaaaag
     4921 ctggcggtgg caaagccatg catctttgcg cagcaaagtg c
//
LOCUS       DAWBSB010000166         4959 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_3012_length_4959_cov_2.680057, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000166 DAWBSB010000000
VERSION     DAWBSB010000166.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 4959)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4959)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4959
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3012_length_4959_cov_2.680057"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3784..3793
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1094 a         1246 c         1588 g         1021 t
ORIGIN      
        1 tgagaacatt gaaccatgca aaaccgcagc aggtgcggtt ttgttggggc cgctttgcgg
       61 cgtcatggta taatagagtc gcaaactacc agagaaaagg tgatgtgcgc tgtctaccgt
      121 tatcacacct gataacaaat tgataactat aggctggatg atatggatat atcaaataat
      181 tatttccaaa gcaaaattcg ttagaatatg atactcagca acgagtggga gtgaagatat
      241 tggaatacag aagatttgac aaaaccatca tagtgcggct ggataagggc gaggagatac
      301 tggagcaggt aaaagagctt gccttgaagg aacggataaa gctggccagc gttcaggcgc
      361 ttggtgcagt cgaccgtttt actgtgggtg tgttcaaaac agatgataag aagtatcttg
      421 caaatgattt cagcggcagc tttgaaatcg tttctctgac cggaacgatc aataccatgg
      481 atggagcgtt ttattgccat ttgcacatga gcgcgggcaa tgataaggga gaggtctttg
      541 gcggacacct gaacagagcg gtggtgagcg ccacctgtga aatggtggtc aatatcctgg
      601 agggaaccgt ggaccggtac tacgacgaac agatcgggct gaatctgctt cggttttaag
      661 gctgtatagg gcagcagttt ttttaccgga agcaatgaag caagcacatg ggaaaatcaa
      721 aacaagcccg aaggacagag accgctgttc ttcgggcttg tttcaaatgc cggcgtgcgg
      781 cggcccgaaa agtggaaaga aggggaagcg catgaacgaa accgagatta ttcaacaccc
      841 gcagatcgac gggctgcgga ttttttttga cacagtggat taccgcacgc cgcatgttca
      901 tgaggaattc gaactgatct ggttgatgga gggaagactc tccgtgcagg cgggaccctt
      961 ccggcatgtg gcacagtccg gtgagatggt gctgttcgac ccgcagcaga ctcatgaatt
     1021 ccataaggtg gaggagagct gcaccttttt gtgcctgcag gttgctccca cgatgcttgc
     1081 gggatgcttt cccgccatac ggaacatccg tacaggcggc gtgtgccccg gcatatattt
     1141 gtctcctgac gtttttgcgc aggtgcagcg cggcctgctg aacgtgatgc gcgcatatct
     1201 ggagcgcccc gcgtgttacg agctgtgctg tgccggacac gtgcacctgc tgatgtaccg
     1261 cctgctggct gcggcgccgc atgttctgct cacggcagag gaaaatgccg aacgcgagcg
     1321 gcggaacgcg cgcctgctgc ggctcatccg ttttgtggac cagaactata tgcacaagat
     1381 acgcctgtcg gattttgccc gggctgagcg ccgttccatg agctatcttt cgcattttgt
     1441 aaaagaggcg ctgggacaga gctttcagga gtatgtgaat acggtacgct ttcactgcgc
     1501 gtgcaagctc atcgccgcgg gacagcggcg aatgctggat gtatgcgtag cgtccggttt
     1561 ttccgattac aggtattttt cagcggcgtt tcaaaagcgg ctgggtatga cgccggagga
     1621 gtacagccgc cagccccaga gcccggtgct ggatgaaatg caggtacacc acagcctcca
     1681 ttcgctggaa cggttttatt cccgtgaaaa aagcctggaa ctgctggaag gtttcgaggc
     1741 gaaatacgct cccggcgtat aaagaaacac ctttccgtct ggacagaatg gcaaaagttc
     1801 aaaaacagct aatttgtcta atttaaaatg cggaatcgtc aaagagagca gggcgccgca
     1861 gcggtatact gaaagagaca ataggaggcg tgtctatgct gtatattggt atcgaccttg
     1921 gcacttcggc ctgcaagctt cttctggtag atgaaaaagg ctgtgtgcgg aataccgttt
     1981 ccaaagaata tccgctctgc ttcccacatc cgggctggag cgagcaagac cccggggatt
     2041 ggtggacggc gtgccgggag ggcgtgccgg agctgttggc cggctttgac gcgcgggagg
     2101 tgcgcggcat gggcgtgggc ggccagatgc acggccttgt tgcgctggat gaaacggacg
     2161 cggtgctgcg cccggccatt ttgtggaacg acggccgtac cgccgaagaa acagcctggc
     2221 tgaacgatac ggtggggcgc ccggtgctca gcgcgcgcac gggaaacatc gcgtttgccg
     2281 gctttacagc gcccaagctg ctctggatga aaaagcatga gccggagctg ttcgcgcgta
     2341 ttcggaaaat catgctgcct aaggactatc ttgtctactg cctgactggt gtgcatgcta
     2401 cggattactc cgacgcatcc ggtatgctgc tgctggatgt agagcacaag tgctggagcc
     2461 gcgacatgct ggctttgtgc ggcatttcgg aaagccagat gccgcgcctg tacgaaagct
     2521 ttgcacccgt gggcacgctg cttccggaga ccgctgcggc attgggcctg ccgcagggcg
     2581 tgacggtatg cgcgggtgcg ggggacaacg cggcggcggc cgtgggcacc gggacggtga
     2641 gcacaggagc ggcccatgcg gacagcaggt gcaatatctc tctgggcact tcaggcacca
     2701 tttttatttc ctcggaaaag tttggcgtgg acgccaccaa cggcctgcac gcttttgccc
     2761 atgcggacgg cggctggcat ctgatgggct gtatgctcag cgcagccagc tgcaacaaat
     2821 ggtggcagga tgcgatcatc ggcacgaatg actacaaggg cgaagaagca aagatcgcac
     2881 ccgaaatgct tggcaggaat catgtgtatt tcctgccata cctgatgggc gagcgcagcc
     2941 ctatcaacga caccaacgcc cgcgcaatgt ttgtgggaat gacgatggac accgcccgtg
     3001 cggatatgac gcaggccatg ctggagggcg tggcgttcgc catacgggat tcttttgaag
     3061 tagcgaaagg gctggggctt tccattccgc gctccaatat ctgcggcggt ggttcgcgct
     3121 ccatgctatg gaaacgcatt ttggccaatg tgctgggaat tccgctggat gtggtgaaaa
     3181 cctccgaagg cccgggctac ggagcggcat tgctggcgat ggttgcctgc ggagaatatg
     3241 agaccgtggc gcaggcatgt tccgcgctgg tgcgcacagt ggataccgtg gagccggacg
     3301 cttccctgac ggcgctgtac gaggaacgct accgacagtt ccgacagatc tatccggtat
     3361 gccgggcgtt gtttccaaag ctgctgtaaa cggcggcccg gtatgcgttt acaaatacga
     3421 aggagagaag tgcgatgaaa atttattctg ttacagacgc ggcgttccgc ccgtacggca
     3481 aaatactgga gggttacgac accgcgcctc tgtgtgcggc catgcggtcc atccccatgc
     3541 cggagcaggg cacggcctac gaaccttcca tccccgtact ggaggaatgt gcgctgtacg
     3601 gcgtgctgca aaataacgcc tatggaggga tgcccataca gctgggcctg tgctggggct
     3661 acaacacaag gctgaactgc ctggaatacc accgggacag cgaggtgaat gtgggcgcgc
     3721 gggattttat cctgctgctg ggaaagcagg acgagatcga aaacggggtg ctggacaccg
     3781 gaannnnnnn nnnaagtaaa ggcgttccgc gtgccggcgg gtatggccgt ggaggggtac
     3841 gccaccacgc tgcactatgc gccctgccat acaaatgcgg cggaaggctt ctgcacggcc
     3901 gttgtgctgc cgcgcggcac caataccgaa aagccgtccg ttgtacagcg cggcggggag
     3961 gatgctttgc tgtgggcgcg caataaatgg ctgctggcac acgctgcgtc ggacgaagcc
     4021 gcgcagggcg cttatgtggg ccttgcagga gaaaatatcg atatttcgga cagcctgtga
     4081 gctggccgga caacagaaaa ggagagatgg agatgtttgc aaatattccc gaagtaaagc
     4141 tgggcatcat tgcggtgagc cgcgggtgct tccctatggc gctgtccgag cggcgccgcg
     4201 cagcgataaa ggctgcctat ggcgagggcc tgtacgagtg tcccgtcacg gtggaaaatg
     4261 aattggacgc caggcgggcc gtggaggatg tgcaggctgc gggcgtgaat gcgctggtcg
     4321 tgttcctggg gaatttcggc ccggaaacgc cggaaacgct gattgccgaa tggttcagcg
     4381 ggcctattat gtatgcggcc gcggccgagg gcgacggaga cctgcacgac gggcgcggcg
     4441 acgcctactg cggtatgctc aactgctctt acaatctggg cctgcgggga aaaacggcgt
     4501 atattcccga atatcccgtg ggtacggcgc cggagctggc tgaaaaggtg gcggaatttg
     4561 tgccaatcgc ccgggccatc ctgggcctga gagggctgaa gctgattacc ttcggcccgc
     4621 gtcccgatga ttttctggcg tgcaacgggc cgatcaaagc gttgtacgac ctgggcgtgg
     4681 cggtggaaga aaacagcgag ctggatttac tggtcgccta caacaagcat gcggacgacc
     4741 cgcgcattcc ggccaaaatg gccgagatgc aggcggagct gggcagaaac aacaagtatg
     4801 cgggtgttct gccccgtctg gcccagtatg agctgaccct gctggattgg gccgaggccc
     4861 acaaaggcgc gcggcagtat gtggcgtttg ccaacaagtg ctggcccgcg ttccagacgg
     4921 agttcaaatt tgtgccctgt tatgtaaaca gccgccttg
//
LOCUS       DAWBSB010000167         4949 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_3020_length_4949_cov_3.891500, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000167 DAWBSB010000000
VERSION     DAWBSB010000167.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 4949)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4949)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4949
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3020_length_4949_cov_3.891500"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          900 a         1536 c         1518 g          995 t
ORIGIN      
        1 cagcaaagtg caggccacgc ggcgaatgcc gccaaaaagg ggtgggggac cgcacccgca
       61 ggcgcgtgtg ggagcggtgc cccccttgcg gacaatcgcg ctgaaagcgc ggctcttagc
      121 gcgcagacgc ccccgcaggg ccagtgccga ggcatgtgca gagcgaagcg gccgccgaag
      181 gccgccgtgc gcggcgaatg gagccaaagg ccctgtccgc aggcagcttg gcgcaaaagc
      241 gcagcttttt cgacaagctg ctctgcatct gcagaaaacc ggacacagaa ggataaagat
      301 aaagcacggc agcccctcgg agcaatgttc caaagggctg ccgtgcttta tcgggtaaaa
      361 ataaattttg aattcacttt gaagcgggtg aagtcgttgc ctacaccaat attgctgtac
      421 ggagcttttt tgcgggtttt atacgggaag cctgttgcag cactctgtgt gctgtccatt
      481 ccgcaaaacg catgaacgcc gtttacagca ccacgccgtc cttaaaaata gagatctctc
      541 ggtatccttt ctgctcggtg cgggtctgtt tcccggaggc gacgtccagc accagcgcgt
      601 acagcgccgc gcccgcttcc ccgatgctct gcgaaccgtc tgctatcgtg cccgcgttga
      661 agtcgatcca gccttttttc ttttccgcaa gcggcgtgtt ggtggcgatt ttcagcgtgg
      721 gggcgggcgc gccgaagggc gtgccgcggc ctgtggtgaa cagcaccagc tgtgcgcccg
      781 ccgccgtgag cgccgtagcc gagacaaggt cgttgcccgg accgttcagc aggttcagtc
      841 cccgggcgtg tacctgttcg ccataaccga ccacgtcaac aatgggcgcg ctgccgcctt
      901 tttgcacgca cccgcaggat ttgtcctcca gcgtggtgat gccgccctgc ttgttgcccg
      961 ggcttgggtt ttcgtacacg gtttctccgt gggccagaaa atattcctta aatccattca
     1021 gcatggacgc ggctttatcg aagacggccc ggctttggca gcggcccagc agcatgccct
     1081 ccgcgccgaa catttccggc acctcggtga ggatggtgct gccgccccgt gcacacagca
     1141 tatcgctgaa ccggcccacc gccgggtttg cggtgatgcc ggaaaggccg tcgctgccgc
     1201 cgcatttcat gcctaccaca agctcacaaa caggcacggc ctcgcgccgg gcctgtccgg
     1261 cgtgggccgc cagttccgcc agcagcgcgc cgcccgcctc catctcgtct tccacatcct
     1321 gacagatgag aaatttcacc cgcgcggggt cataggggcc gagttcttct gcaaattgtt
     1381 catgggtcag attttcgcac cccagtgaca gtaccagcac gccgcctgca ttgggatggc
     1441 gcgccagcgc ggccagaagg cggcgcgttt gggcgtggtc ggctccggtc tgggaacaac
     1501 cgaagggatg gggaaatgca tacagcccgt cgatggaacc ggagacaagg ctctggttct
     1561 ccgcggccaa ggccttggcg cagtcgttta cgcagccgac ggtgggaatg atccaaagtt
     1621 cgttgcggac gcctacgcgc ccgtccgccc ggcggtaacc ctggaatgtt tcgggcggca
     1681 cgggggacag gggacgcacg tccggcgcat aggtgtactc cacctcgccg gacaggttcg
     1741 tgcgcacatt gtgggtgtgc acccatgcgc cgggcgggat atccgaagcg gcgtgcccga
     1801 tgggatatcc gtatttgatg atgttttcgc ccgcggcaat ctgggcgagc gccagcttgt
     1861 gtccctgcgg gatgtcctcc cgcatttgga cggcgcggcc ctcaagggag agccgcgtcc
     1921 cggcggggat ggggcacagc gcgacggcta cgttgtcggc cgggtggatg cggatgcatt
     1981 gcggcatatc gggcctcctc ctttacactg cgggacgggc caggcacgcg gcgaaagccg
     2041 cgcgggcgcc ttcggtacgg atgcggcgca ggttttccgc agcggcctgc tcaaagccgg
     2101 gcaccagcgt caggtcctgt ccccacattt tttcattcgt catcacgtca tgcaccagcg
     2161 tttcgtccga aacgccgcgg cggctgtaat agaattccag cacccagcgg tcatcgctga
     2221 cggtgtattc gttgcccttc ggccggcggc atacaagccc ctgttccgtc agggcctgga
     2281 tatcgctgga atagaaagcg atgtatgcgg cgaagctcat cgcaaggcac gggggcagct
     2341 ttccggccgt ttgtgcatat tccagcaggc tgggcatatt gcgcgcgcgc cacttggagg
     2401 tggagttcag cgtgatgctc atcagctcgt ggttgatgaa cgggttgttg aagcggtcct
     2461 gcacggcggc cgcgaaggct tccagatcct gcctgtccag cggcagcgtg gggatgactt
     2521 cctcgtgaag catgcggttc atgaagccca ggatcacatc gtcctgcatg cagtcacgta
     2581 caatgtcgta cccggccaga tacgccccaa gcacaaagcc tgtgtgcgcg ccgttgagga
     2641 tgcgcacctt gcgttttttg tagggcgtca catcgggcac cacggggcag ttcagtcctg
     2701 cggcccggaa tggcagcttt tccgccagcc attcgggccc ttcgatattc cacacgccga
     2761 acacctcgcc cacgtcgatg agcgcgtccc ggtagccgtt ctcatcctcc agccgagcgg
     2821 cctcggcggc gtcgcgaatg cggccgggta cgatgcggtc caccagcgtg gagcagaagg
     2881 tgcagtcccc gttcacccag cgggcaaagc cttcctccag cccccactgc tttatgtatt
     2941 ggttcacaca gcgcagcagc tccttgccgt tgttgtcgat gagctcgcag gagagcacca
     3001 ccacgccggg cctgcccgct ctccagcggt gcagcagaac ctgtgtcagc tttgcgggaa
     3061 aggaagcggg cggacagtcc tcaaggcggc acgaggggtc gtacacgatg cccgcctcgg
     3121 tggtgttgga gacgatgtat tccagcgctt cgccggcggc tacatccatc atggcgtcgt
     3181 aatcctgttt ttcatagggg ttcaggcagc ggctcaccga ggagatgacg cgctttgcat
     3241 ccaccttttc gccgttctgc cgtccgcgca ggtacagcgt gtacaggccc tcctgccggt
     3301 tgatgagctg cgtcagcccc tgggcgatgg gctgcaccag aacgcatttc ccgttccagc
     3361 ccgccttttc gttggccatg tcgaaccagt agtccgcaaa ggcgcgcaga aaatttccct
     3421 cgccgaactg cagcacgcgc tcgggcgcgt ttttcagcag atagccggta tatccggcct
     3481 tttcaagtgt ctcatagcac agttgttcca tttttgcttc gctccttttt gatgaattca
     3541 ggggcagacc gccccggatg cacggtaata ggcaaggctg cttttgaaga tcacacggct
     3601 ttccacgtag tggacgccgg ccgggggctg tatcccgcgc agcacgaaat cacccataag
     3661 ccgggcagcc agatatccct gctgcgccgg gtccttataa agaatgttct gcacaaggcc
     3721 ctcctccatg ttgcggacag tctcgccgaa caggtcgctg gcaacgacgc gtacctgccc
     3781 tgcccggccc atttgggcga cagcatccgc cagcagcacg ctcaaccgcg cgctgacgct
     3841 gaatgcgccg gaaatacgtg cgtcttccgc aagtgtccgt ctcaggcgca gcgccagctc
     3901 ctgcttgtca tgatagccgt cggccctgac cagagtaagc ggaacgctgt tctcccgcag
     3961 gtatgcttca aatccggccg ccatccggta gtgggagggc gtgtgcgcgt cccccgcgca
     4021 cagcaatacc gcatcgccgg gtgaaagctg cgcgctcaga agctcggcgg cgatacggcc
     4081 ggccatgtcg tggtccgcct gcacgcaggc gatgcggccg cagccttccg cgtcgtcaca
     4141 ggcgaagaac acaggcgccc ctgcgctgct gtaccgctgt accgcccgcc gggcagtgtc
     4201 ctccgtgggg ccgacggtga gcaatgcgtc tatttttccc ccgtactgct caaagcaggc
     4261 gttcagcgtt tcggcctggc cgcgctctgt gccgggataa tagggcagct ccaaaacttc
     4321 cagattgtag tcgtccagct cggccatgca gcggcgaaac ccgcgccaca cgctgccgta
     4381 aaaataggag ttttcttcct gtgggccggg aaacgcggcc gcgatgcgca gcgcgctgcg
     4441 cttgagcgcg gacgcggcgg aattggcgcg gtagccgcgc cgtgcgcaca aatccaatat
     4501 ctgttggcgc gtttgctcgg aaaggccttt tttgccgtac agcgcccggt ggacggtgcc
     4561 ggtagaaacg cccgcctcgg cggcgatttc tttgatggtc acacggtgct ccatgatcct
     4621 gctccttttg cggcacggtt cctacaatgc gtaggtaaaa cgttttagta aaaatcctgc
     4681 ttttatcata acaggcaaag cggcgatatg caagaggaac aatgtaaatt tctcacacat
     4741 aggctgaatg ccaaagtaaa ggagagcttc ggaaaacgac cgttttgcag aaaagattcc
     4801 cgttttgcgg acgcgcccat gtgccggttt gcgaaatgct gcgggtgttt actacgcaaa
     4861 ttccgatttt attatggctg cggataccgc gtcatggtat aataacctca aagctgccgc
     4921 ttgcgcggct gcgcgtgtgc aaggcacac
//
LOCUS       DAWBSB010000168         4929 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_3034_length_4929_cov_2.771851, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000168 DAWBSB010000000
VERSION     DAWBSB010000168.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 4929)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4929)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4929
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3034_length_4929_cov_2.771851"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1056 a         1627 c         1283 g          963 t
ORIGIN      
        1 atccggttgg tcgaaagccc caatacaacc gagaccagat cggtaatgcg ttttatcaca
       61 gtaatatcgt ccccgccctc gcacaccacg gccacgccct tcacctccgg ttcgatgtgc
      121 gtttccacaa gaggttgttt tccatccgcc gcgtcaacga tgatatgctc gcttttatag
      181 gattcctttc ggtccgtcac accgtccgat ccgtcctgcg tcgtctgtgt atcagattgt
      241 tcgtcgcgtg catacaccgt ttctccggcg ctttccagtg tgatcatcac ctgcactttg
      301 ccggcgccct gcacgctctc cagcatttcg gtcaggcggg tctccaattc ctgaacgccc
      361 gtcgctgcgc tcacagcctg ctgtgtgctc tgccgcgccg cgcccgtgga accctcccgg
      421 cctccgaaca gaatgtcgga taaaaaaatc aggcagatcc ccgcgagccc gacaaagaaa
      481 agccaattct ttttctggaa cagcccgtgc agcatttcct gccagccccc atgctccgct
      541 ttcttttcca tttgcattat ccttcttcat ttaccgttac cgggacatgt gttcccagcg
      601 cttttgtgac aacatcctcc acgcccgaag catctgtgcc gccggggact attacagtga
      661 cgtccgccac ctctacggtc tcgctgtcgg cgtccaacgc cacgtccact tcggtgtacg
      721 caatcccgtt ctccgcaagc gcgctgcgca cagtatcctc caaccgtgtg cacaccgtgt
      781 tcaacaccat tgcttccgta tccggcgcct gcactgtctg cggcgcttcc ggaatgtcga
      841 acgttacatg cccatcgccc aggctttgca gcggggcgaa tgcggttatt aaaatatata
      901 gggtgagtat cagctttatg acagaaaacc gcgtccggga ggaagaaagc tgcccgagga
      961 tgccagccgc gatacacgca aagcacacgg ccagcaccca ggccctgatt tgttccaatt
     1021 ctaccctcca ttccccagca ggatcatcac aagcgtggaa gaaatgacga tcaggctaaa
     1081 aaagacaacc atcgaaatgt aaaggctcac acaatccgag aggccggaaa gcagctttgc
     1141 gcttttggtg ccgcccgttg ccgacgccac gatgtttccg gcggaaaaca caaggtggta
     1201 cgcacagcac tgtatgagca gcggcagaaa cgacgccgca atgacggcga tcaccgcaaa
     1261 ccccactgtg cctttgagca ttttcaatcc ggccagcaca ctgcccattg cgtcggagat
     1321 tgcccgtccc acaacgggca cgccgctgct cagcagaaat ttcccggttt tcagcgcgag
     1381 tgtatccgcg ctctgcgcaa atatcccctg caggccgatg agcgcgccga acaccgtggc
     1441 gcaaaacgtg agcagccact tcgtccatcg gcatacggag gcggtcaatt gcgaaaggtc
     1501 cagcgtactg cctacggccg cagtagcatt cagcgccagc agcacacgcg tgagcggcag
     1561 ccccactctg cacagaatat ctgccataac catagccgca ccgaagaaaa agccactgta
     1621 cagcgccgcg ccgcctacct gtccgcaggc tgtcagcaca gaagcgaaca ccggaacaaa
     1681 agaaatgata taaacgcggc ttgtttcgat tgcatcctgc agtacaccgg tgagcgccag
     1741 catgcaggaa gaacagagcg tgaacacagc cagcgtgaca acagtatcca caaggcccgc
     1801 gagttccggc gacgttcgct cggtgcacat gctttttccc acggccgcca gcagcagcag
     1861 gccgcacagc cttgccaata gtctcaaagg ctcatgcaag ctctcgcgca gtatttccgc
     1921 gcccttttcc agccagtcgg acaatgtcat cccgctgaaa tccccggcgg tcattggtgc
     1981 ttcctgcgtg acctgcgccc attcctcagg tatcccggat tgcccgcttc cggagtctcc
     2041 cgccgcctcc tgcgccagcg ccggcacgcc ggccgccgca acgcagaaca gcatggctgc
     2101 aaggcaagcc agccgtttcc aaatccttcg tgagtgaagc ggctccttca tcgcagcagc
     2161 gccagcagcg tatccgtcag caccgtgaac agcggcatag ccgccagcag cacggccacc
     2221 ttgcccgcca gctccacgcg tccggcaagg gccgtttgcc ctgcttccat gcacaggtcc
     2281 tgcacgcttt gggccatcag cgcgatggcg acggccttga acacggttcc caaatgctcg
     2341 aagcctgaaa actgcgaaag agtttccaca aaagaaagta caggccccag cgcctgcatg
     2401 acgaaccaaa gcaataccat gcagcaggcc agtgcggcca gcaccgcgta tccgggattg
     2461 tattggcgca gtacgctcaa cagcaccagc gcacacagac agaagcccag gatcttgaaa
     2521 atgtccatgg caggtcacaa tgcaaacagg tccttgatgg tgtcgaacag gacgctgatc
     2581 tggctgatga tcatcatcag cacgacgata agccccgcca gcgtcgtcat catggcctgt
     2641 tcctcccggc cggaacggat cagcagctgg ttgagcacag ctacaatgat gccgatggcg
     2701 gcgatcttga atacaaaatc aatgttcatt tttcgcttcg ctcctttttc acatacagcg
     2761 tgctgcccgg aacgccgcct ggctcacagc agcagcagcg cggcagcccc gcccagcgag
     2821 aggcccagcg tccggtacag ccggcgctgc tgggcagcct ccgctctggc agcatccaaa
     2881 gcggtttcca gctgtgtttg ggcgtaggcc agcctttgtt cctcctcctg cgcaggacag
     2941 ccgcccagcc cctccagcgc gccgcgcagc agcacgcgct cttcccgccg cgtcacgccc
     3001 gcaatttccc gttcagtgcg gcaaagcgca ttgtccagct ctgtctgaag gccgcagccg
     3061 gaggcgagat cggaaaaata aagcggcagc gccgacagcc gggtattttg ctgtgccgca
     3121 gcgaggattt cctccgttcg gccgcagcga aaatgaaggt cgtcccgcac tgcgcccaaa
     3181 aaagcgcaga tatccgccag cacctgtacg cggcgcatgg cggcgttgcc tttgcgccag
     3241 cccatggctg cgcccgtgta aagcacaagt atcgcaccga tacatttaaa aaacagttcc
     3301 cgcatccacg acctccgata ttctgcccgg tgtttcctta ccttttaaaa acgccacatg
     3361 acggaaagcg cccgtctgca gcagagccat gtactgcggc ctgcggcgca gcgtttccgc
     3421 gtcaggcgcg tggatggtca ccacaagccc gacgcctgcg tttgccgcct gcgtgatcgc
     3481 cgcgatatct tccattgctc caacctcgtc acacacgata acgtccggtg caagtgcgcg
     3541 cagcgcatgc tgcataccga tgtgtttggg gtatcccgaa agcacgtcgc agtgctgagg
     3601 cagccgcgtg cagaagccgg actgctccac aggggcgatt tcgaaacgct cgtccactac
     3661 ggctgttttt cggcccgccg cggaaagctc cgccagcacg gcgcgaagca gcgtcgtttt
     3721 gccgctgccc ggcgcacccg cgatcagcag ccccacgccg ggtgcgcaca gcagcggacg
     3781 cagaacggcg tcgcaggcac acaagcccgt gcgagccacg cgcagattga tggatgtgat
     3841 atttttgagc gtaaaggttc cgctgccatc cacgaatgcc gtaccgcaca cgcccgccct
     3901 gtgcccgccc ggcagcggaa caaagccgtt cgcgatgcaa tccgcaaaac tgtgcaccga
     3961 atatccgcag atggcctgaa agcagttctg caccagcgtg tgcggcgtag acagcacgcc
     4021 tgtatccggt ttttctcgcc aaccgccgtc tatggaaaca aaccgtgcgc cgctcggcgt
     4081 gctaagcgac accgggcggc ccgaacgcaa gcggatttcc gtaatgtccc ccgcccaccg
     4141 gggcggaatg cgcatcagcg cctgcgagac tgcaccgggc agctgtgacg cagcatgata
     4201 aaaagcgtcc atctcttccc gtcctttgcc attcatttgt tcaatgatat gaaacgcgga
     4261 caggcgttag aaccaaaaat gccccgggcg ctgaatgcgt ccggggcata cctgtattgt
     4321 gttattcgtc gttttcctgc acgtaagctt cctgttcttc cacaagcctt tcccattcgg
     4381 ccaggacctc gtcctgatga aaacgggtat cgtccagctc atcgcatacg tcccgcagac
     4441 gcgtgtgatc ccgcagaaca tcagggtcgt tcacttcacc ctccaacacc atagtgcgca
     4501 gtgccagagt ctccatttca tcctccagcg ctttgatccg tgtgcgaagc tccgcgcggc
     4561 ggcggcgctg ctccttgccg taagcagcct tgcctgcaga cgccgtcttt tccggtgcga
     4621 tgtttttttc aggcggcaca gccccgcggt gcatcatcgc gtcataatct tcatatagcg
     4681 aagtctttcc gttttcaatg aataaaattg gacaagcaag gctgttcatc agataacggt
     4741 catgagtgac gagcacaagc gtcccctcat aggaagccag cgccgccccc agagattccc
     4801 gggtatagat atccaaatgg ttcgtcggct cgtccaggaa caaaagattc ggcttttcca
     4861 gcaaaatttc cgcaaagcgc agacgggcca gttcgccgcc cgaaagtgcc gaacacggct
     4921 tgaatacat
//
LOCUS       DAWBSB010000169         4916 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_3045_length_4916_cov_4.390249, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000169 DAWBSB010000000
VERSION     DAWBSB010000169.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 4916)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4916)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4916
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3045_length_4916_cov_4.390249"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          899 a         1420 c         1679 g          918 t
ORIGIN      
        1 gggcgacctg acgggcagcg gacgtacgga gctggcggtg gccgccgcca catcggagcc
       61 gggcgggctg tccctgcagc tttttgtgcc ggacgggcgc gccatgacgg cggcacaggg
      121 cgtagagctg gacgagcggc tggaaaaatg caccgctctt tacgcgaccc gctgcggcgc
      181 ggcccgcggc cttattgtgg acggttcagt gtcctcgggg gcggccagcc agcttctgtg
      241 ttgggaagac ggcgtgctgt ttccctatcc ggacaaagag gaaggcggcg acgtatttgc
      301 ccgcagccag cgctcccgca ccctgtccgc gttggggccc caggaccttt cgggcacggg
      361 cgaggtgctg atccccgagg tgggcgccag catcgctacg ctgcagtcgg cccggcgttt
      421 ttatcccgtg gagtggatga actacctgtg cgagcagccg ctgcagcagt tcggcgtcta
      481 cgacgcggat cacaactatt ttgtgcgcct gcccacagaa tggcaggata atatgaccgt
      541 atccgcccag tccgagagcg attggcagat acggtccaaa gaggacaaca gcctgctgtg
      601 cgcggtgcgc gtggcagacc gcgccgctgc caacggcacc tatacacagg cggcgcagct
      661 ggcagagaaa aaagtgctcg tgtatttcgg cgaaagctgc actgcggtac aggcgaacct
      721 gatccgccgc ggcgtggctg tgctgggatg accgggcttc aagacatcct tcgggaggaa
      781 tgtacatgag aaagatcctg gttgtggaag acgaagacgc catccgcgag gtggttgcgc
      841 tcaacctgcg cctcgccggc tacgaggtga cggaggctgc cagcgccgag caggctctgg
      901 cggtgttcag cccgtcggcg ggcttcgacg tggccgtgct ggacatcatg ctgcccggaa
      961 tgaacggttt ttccctgtgt gagaccatcc ggagggacag cgcgtccatc ggcatcatca
     1021 tgctcagtgc aaaaacgctg gagacggata agatcaaagg cctctccatc ggcgcggacg
     1081 attatatgac aaagcctttc agtgtcagcg agctgctggc gcgcgtggaa gcgctggtgc
     1141 gccgcgtggg gcggcagggc gcctcctcgg ccgcgccgga gccggatggc cgcatggtgt
     1201 ccggcgtatt cgtgctggac cagaaaagcc gcgtgctgta caagaacggc acgcccatcg
     1261 accttacgca ggtggagttc cagatcatgg agctgttttt tgccaacccg ggcgtggcgc
     1321 tggtgcgcga gaagattctg gagggcgtct ggggcgaaaa ctatttcggc gacgtgaaga
     1381 tcgtggacgt gaacattcgg cgtctgcgca tgaagatcga ggacgagccc agcgcgccca
     1441 agcatatcct gacggtgtgg ggctacggct accgctggaa cgcatagcac tggttttgta
     1501 ttttctttgg gcagaggggg cggccgtgtg aacagcatca ccaagcgctg ggtgcgcggc
     1561 agcctgcttg tcaccattct gatgtgcctc gtggcggagg ccgtgttcct gtattattcc
     1621 atcaccaatt actacgacgg cgcgcggcgg gctatgacga cgcgcaccag caccatcatc
     1681 acccagctca acgcaagcgc ggcccagacg ggcgagagcc agggcgtgct tttgcgccgt
     1741 atggtggagc aatttacgga gaaggacaag ttcgagctga tgcttgtgaa cgctgctgga
     1801 aagcctctgg tgacaagcac aggatttgcg ccggacgaca gcgtggccgg agaggattat
     1861 ctggaggcgc tggccagcga aaagggcgag gggcacgcgg tgtaccgcac ggccatgggc
     1921 gaaaaggtga tggccgtcac cacactgctg ccccgggccg cgggagagat cgtggctgtg
     1981 cgctttgtca ccagcctcac gctggtggac cagagcatcg cggcgctggt ggctttcagc
     2041 ctggcgctgg tcacggcagt ggtgctgttc agcgtgtgga cgggcatgtt cttcatccgc
     2101 agcatcgtgc gccccatcgg agagatcgag gctaccgccg cgaagatcgc cgagggaaat
     2161 ctggacacgc gtatcgaaaa caaatataat gatgagatcg gcaagctgtc cgacaccatc
     2221 aaccatatgg cgggcgagct ggacaagacc gagcgcatga aaaacgagtt catctcctcg
     2281 gtctcccacg agctgcgcac tccgctcact tccatcaagg gctgggtgga gaccatcgcc
     2341 gccatccgtg acccggccga cccaaatttc cgccggggcg tgcaggtcat ttccagcgag
     2401 gcggaccgtc tgtacagcat ggtggaggag ctgctggatt tttcccgtat gcagaacggc
     2461 ctgaagctgg acctgcagct tttggacctg gtggccgagg tgagcgacgc ggccatcatg
     2521 gtggagcgcc gcgtggagct ggagggcctg catcttgcgt atgacgaacc cgaagagccg
     2581 atgccggtga tggcggaccc cgcccggctg cgccaggtgt tcatcaacgt tctggacaac
     2641 gccgtcaaat attctccgcc ccacggcact gtacgtatgg acgtgctgtc cgacggcaac
     2701 agcgctttcg tcaatatctc ggatgaaggg aggggcattg cgccggacga tctggaaaac
     2761 gtcaaggtga agttttttaa gggaaaaggc gcggtgcgcg gaagcggtat cgggctggct
     2821 gtggtggatg agatcgtcac cgcactggac ggcagcgtgg acatccaaag cgaggtggga
     2881 cgcggcacga cggtgaccgt gcgcctgcct ctgtacaagc ggccggctcc gtccggcgcg
     2941 ccgccccgcg gcggcacggc gggggtttga aacgctttcc cggcgtcgcg ggcctgcccg
     3001 cgcgccgtga aacgcagtcc ggcctttggg ccgggagcgg ataatttatt gatgcatcgt
     3061 ccgcagaaag gaaatccgat tgaacaacaa aaatgaaaaa gcctgctgct ttaaaacgag
     3121 cgtgggcgga caggccctta tggagggcgt gatgatgcgc ggcccgcaga aaatgtgcgt
     3181 ggctgtgcgc cagccggacg gagagatttt taccgaggtg tcgcccatcc ggacgcatcc
     3241 ctggcagaaa tggcccttcg tgcggggaat attcaacttt atcgacagcc tggtctcggg
     3301 ctacaagtgc cttatgaaga gcgccgaagt ctccatggga gaggacgcgg acctggagga
     3361 aacaaagttc gacaaatggg tggccgaaaa attcggcgac aaaggaacca atttcatcat
     3421 ggccgtggcg gccgtgctgg gcggcgttct ggcgcttgtt ctgtttatgg tggtgcccac
     3481 tgcgctggcc gggctgctgg cgcggttcgt gccgctgggg ggctggcgcg cgttgattga
     3541 gggcgtgttc aagctcgtca tgctggtggg ctacatggcc ctggtcagcc gcatgaagga
     3601 gattgcccgc atgttccgct accatggcgc ggagcataag accattgcct gttatgagtc
     3661 aggccgggag ctgaccgtcg aaaacgtgcg cccctgtacc cgtttccatc cgcgctgcgg
     3721 caccagcttt ctgctgatcg tcattctcat cagcattttg gccggcgcgc tgatcaccac
     3781 cgaaaacaca ctgctgcgca ttgcgcttaa gctgctgctt ctgcctgtcg tgatgagcgt
     3841 cagctatgag atcatcaagc tctgcggccg gtacgacaac ccgctcaccc gtgcggtgtc
     3901 cgcaccgggg ctctggctgc agcgcatcac gaccagcgaa ccggacgacg gcatgatcga
     3961 atgcgccatc gccgcggtgc ttccggtcct gccggaaaat cccgaagacg gaaaatggta
     4021 acgcccctgg cggcggtgcg ggacaggctg gaggccgcag gcatcccgga cgcggcgttc
     4081 gaggccgcgc agctttacat gcttgccacc gggcgggacg cacggctgtc cggcggcgcg
     4141 ccgctgacgg cttttgaaca gaagaagctg gacgcactgt gtgaaaaacg gtgtgcgcgg
     4201 ttcccgctgc aatatctgtg cggcgaatgg gactttctgg acctgacgct gaaggtgggg
     4261 ccgggcgtgc tgattccccg ggccgacacc gagaccgtgg ccgaggcggc cattgaagcg
     4321 gcgcgtgaag ctgggccggg cgctgctgtg gcggacctgt gcagcggcac cggagccatt
     4381 gcgctggggg tggcaacgca tgtgccgggt gcgcgcgtca cggccgtgga gctctcaccg
     4441 gacgcactgg cctatctgcg cgccaacaat gcggcctgcg gctgtccgct gtgtgtggtg
     4501 caggcggatg ttctgcgctg gcaggaggaa tgcgcgccgg gcagctttga tgtcatcgtg
     4561 tccaacccgc cgtatatcgc gccggaggag atggccgggc tgcaaccgga gcttttccat
     4621 gagccgcgca tggcgctgga ggccccggac gggggcttgg ccttctacaa gcatatcgcg
     4681 cctgtttatt tcggcgcgct caaaccgggc ggccgtctca ttctggagat cggctggcgt
     4741 caggcggacg ctgtgtgtgc tctgtgcctt tctgcgggat atgaggatgt gtctgtccgc
     4801 agtgatttga gcggcaaccc acgctgtgtg ctggcccgcc gtccgattgc attttagcgg
     4861 cttttggggt ataatacccc cacccccgcc gctgccgcgg ccacgcgcat tgcatg
//
LOCUS       DAWBSB010000170         4807 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_3111_length_4807_cov_2.212542, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000170 DAWBSB010000000
VERSION     DAWBSB010000170.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 4807)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4807)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4807
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3111_length_4807_cov_2.212542"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1813..1822
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4234..4243
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          989 a         1370 c         1317 g         1111 t
ORIGIN      
        1 ccaccaagga ggcgagcgga aacgtgagcg ccatcgcgaa aacggctgcg ctgtgcggcg
       61 acgaactggc catctattcc ggaaacgacg accagatcct tcccctgctg gctctgggcg
      121 gcatcggagt gatctctgtg ctggcgaacg tagccccgcg ggaaacgcac gatatctgcc
      181 ggctttattt cgagggcaaa cacgcggaaa gccttgcgtt gcagcttcgg cttctggaac
      241 tgatcgaagc attgttctgc gacgtgaacc ctattcctgt aaaggaagcg atgaatctgc
      301 tgggacatga cgcgggacaa tgccgtatgc cgcttgctcc tttgaacgca gcgaaccggg
      361 aacgtctgcg cacggcgctc tgtgcggccg gtttcctgaa agcgtaaaac aaatcgacag
      421 gctgctctcc aggagcatgg ctgcacctgc ggccgaacct gatgcagccc cagcttcgca
      481 acagcttacc tttggttttg ccatcgggta agctgttgtg ttatacttat atcatccttt
      541 gaaaaggaag tgtgaatatg accgatatca tcatccaggg catccacggg cgtatgggcc
      601 gcgcgctgtg tacaatgatc gaggcgcgca cagactgccg tgtggtggct ggtgtggacg
      661 cgaaagagct tccaagcgct gttccggtgt tcccgtcact ggagaaaaac accgtgccag
      721 cggacgtgct gatcgatttt tccaaccctg acgccacgca gaatgccatt gaatactgca
      781 gagcgcacaa gctgccctgc gtcatatgta caacgggcct gagcgaggga caggaacagg
      841 cgctgcgtga gctttcaaaa accgtagcgg tgttcaagag tgcgaacatg tctttgggtg
      901 tcaatctgct catcgagctt gccaaacagg ccaacgctgt gctggggaca gatttcgacg
      961 tagagatcat cgaaaagcat caccataaca agctggatgc gcccagcggc acggcgttga
     1021 tgattgcaga tgagctttgc ggcgcgtccc cggttcccta ccactatgta tatgacaggc
     1081 acgccgagcg gcgcaaacgt gaaccgacag agatcggcat tcacgctgtg cgcggcggca
     1141 gcatcgtggg cgagcacgag gtgatttttg ccggaccgga ggaggtcatc accatttccc
     1201 acagcgccgg ttcccgagag gtgttcgcca acggcgcgct gaacgcagcg gtgtttctgg
     1261 cgggaaaacc cgccgggatg tataatatga aggatctgat gcatctatga aacagagaaa
     1321 ccaaaaacac atagccttgc tgagcgtggt gcttgtgccg gccttgctgc tctccgcttg
     1381 cggaagcggt gcatcgggac agctttcgtc ttccagcacc gctccgggct ccgagcccgc
     1441 cgtttccgtt tctcagcccg agccggaacc gcagcccgcc gtgacaacgc tgcgcttttc
     1501 cgccacaggc gacaacctga ttcatgacgg tctttatctg caggcccgca gccgtgcagg
     1561 cggcgaaggt tatgatttta cggctttgta tgaaaatgtc gcgccttttt ataaagactt
     1621 cgacatcaac tggatcaatc aggaaactct ggtaacggac gagcttccgc cttcctccta
     1681 tccctgtttc tgtacgcccg gcgcgttggg gcgggcggta tatgatgtcg gctggcgtgt
     1741 gatctccatg tcaaacaacc actcctacga caaaggcagt cagggtatcg cagcaacgct
     1801 ccgtttctgg gannnnnnnn nnacgtggtc accacgggcc tgtacgcagg cgaagcggat
     1861 tacggcaaca ttcccatcca ggaaaaggag ggcgtgcgca tcgcttatct ggcctatacg
     1921 gagacgacga acggcctccc cacgccttcc gatgctgccg ccaatgttat ttataccagc
     1981 caggaggatg tgatccagca gcagatcgag cttgcacgcc aacaggcgga tatcgttgtt
     2041 gtgggtgtac actggggtgt tgaaggcagt catactccca ccgacgccca gcgtgcgctg
     2101 ggacagaaaa ttgcggactg gggcgcggat gtcattattg gcacgcaccc gcatgtcatc
     2161 cagcccgtgg agctgcttac cgcagccgga acgggcaaaa cggttcccat tgcctattcc
     2221 ctgggaaatt ttgtttccac acaggctgcc gcaaacaaca tggtcggtgt gatcctgacc
     2281 tttgatatca ccaaaacaac acagccggac ggctccgcca gcgcgtcggt gggtaatgtt
     2341 catgcggtgc cgatggtgat gcattatgat tccaactaca ccaatgcgcg cgcctatctg
     2401 taccgggatt acacggaaga acttgctgct gctcatggtg tgcgcgcacg cttttccggt
     2461 ttcagtaagg aatatatcac acagcttttg acagaataca tcagcccgga atttctcgta
     2521 cttgaataag gaggcattgc tatgaacggc gtaaccagaa tttccagtga gcaggacgtt
     2581 atgatgatta cttttacaga ggccgatacg gcctcggctc ttgcggacgc cttgaccgcg
     2641 tttgcgcaaa aaggcgttgt tgtagacatg atttgccaga gcgcgccccg tgccgctacc
     2701 atcgacttca gctttacaat gccggcgtct tattttgaat ccgccatgca gaccatctcg
     2761 gagtaccgca cgggcgcggg cagcgcgcct cttatcagca gcggatacag caagatcaat
     2821 ctttttggtg aagagatggt gacaagctgc ggcgtggctg cccttgctct gcgtacgctg
     2881 cgggatgcgg gcatcgaggt agtgctcatc acgacgtccg atctggatat ttctctgctt
     2941 gtacgcgggg aaaatgagga cagcgcgctg aatgtgctgc gtcaggcatt tgccgcataa
     3001 acggacatat taaaaaacaa gccgggaccg cccgctgcgg agcatcccgg cttgtttttt
     3061 ggttgttttc cggttacagt gtaatacggt gatagacttt cttacctttt ttgataacga
     3121 tgccttttcc caacgcttcg gcgttcacgg cggccgatac atccgtcacc ttttcgccgt
     3181 ccacggaaat gccgccctgc tgcaccaggc gccgtgcttc ccctttgctg ggcgcgaggc
     3241 ccgcggcaac gagcaggtcg agcacggagg cctttccgtc cgccacaagc gccgcgtcca
     3301 caacggtggc gggcatatgc tccgcatcca tttcaccgct gaaaaggcgt tttgctgtgg
     3361 caatggcttt ttcggcgtct tccttgctgt gcacaaggct cgtcagctca taggccagca
     3421 gttccttcgc cttgttcacg tcggaggcgt tgcccatgtc aatcgcgtcg atctccttga
     3481 cgggcacacc ggtgagaagc ttcaggcaat tttgcacctc acaatcgtct atgttgcgcc
     3541 agtactggaa gaactcgtac gggcttgttt tttcggggtc cagccagact gctccttttt
     3601 cggtcttacc catttttttg ccttcgcgcg ttgtcaaaag cgtgaacgtc atgccgtaca
     3661 catcgcggtt atcacatcgg cggttcagct ccacgccgcc gatgatattg ctccactggt
     3721 catttccgcc gaactgcatt ttgcagccgt actcccgcgc caagtgcaga aaatcatagc
     3781 tctgcatcag catatagttg aattccagga acgtgatgcc tttttccata cgtgttttga
     3841 cgcactcggc ggtgagcatc ttgttgacag agaaatgcac gccgatctcc cgcagaaaat
     3901 ccacatagtt caggccgaga agccagtcgg cattgttcgc catgatggcc ttgccctcgg
     3961 aaaaatcgat gaatttgctc atctgttttt taaattcgtc cgcgttgtgc tggatctgtt
     4021 cctgggtgag cattttgcgc atgtctgttt tgcctgtggg gtcacccacc atcgtggtac
     4081 cgccgcccag caggacaatg gggcgatgcc ccgcctgctg catgtagcgc atgatgataa
     4141 gctgcatgaa atggcccacg tgcaggctgt ccgccgtagg gtcaaagccg gtatagaaag
     4201 tgattttttc tttgcccaac agctctcgaa tggnnnnnnn nnncctctgc caattctggc
     4261 agagggcgaa aaaacgcggt accacctctg ttcaccggcc cctcgcggat gccggcctcc
     4321 gtgtgtccag taacacactg cgctttaacg ggcgcacccg gcaaggccta ctgaatcact
     4381 tgttcagccc gctgctcagg gatgtattcg ccatgcgcac cgctcccctt ttcaccaaac
     4441 agaggttctc tttgccggcg ctttcacagg tactcgttcc cgtcgctgca tttatggatt
     4501 atagtatagc caaaatacgc gcggctgtca agcctttgcc atccgaagca tttcccgggc
     4561 attcgccagc gccagttccg taattttatc gccggaaatc atgcgcgcca gctcctgcac
     4621 acggccctcc ccgctcagtg cgtctatttc cgtataagta cggtcttcac tgacgttttt
     4681 tcggatgagc aggtggttat ctgcctgcgc cgcgatctgc gcagtatgtg tgatgcagat
     4741 gacctgtctg ccctgcgccg tctgcttcag cttttcaccg atgcgccctg ccgccagacc
     4801 ggagacc
//
LOCUS       DAWBSB010000171         4735 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_3159_length_4735_cov_2.961966, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000171 DAWBSB010000000
VERSION     DAWBSB010000171.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 4735)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4735)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4735
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3159_length_4735_cov_2.961966"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          927 a         1549 c         1318 g          941 t
ORIGIN      
        1 ccccagtact gcgcaatgag cacgctgccg ccggatgaaa ggccgaagcc gaacgacatc
       61 aaaatggaaa acggctgtcc gcccaggttt gccgcggcaa tggccacatc gccgaagctt
      121 cccagcatca cgctgtccat aacggccacg ccgaaactga tcatattctg cagcgcaatg
      181 ggcaccgcaa tggccagcag cgtgcccacg aaggcccggt tgagcaaaaa atgtttgtcc
      241 agtctctgtt tcacacaatt ctccttttct tttcccggtt tcccgtcctg cctgccgcgc
      301 cgcgtcagta gcgcagaaca aaacagacca catacaccca gctcaaaagc ccatgaaaaa
      361 tagcccagcc caccgaatgc caggtggtgt agctgatgac catggccagg gctctgccga
      421 aggtgatgcc cgttttgatc gttttgttca cgatggtcgt tttgttttct ccgttcatgt
      481 tctccctttc ccgcctcgtc agtgcgcctg ctccgtcttg ggctgcttca tcgtcagggc
      541 gatgcgtttt ttctgcagat ccacgctcag cacccaaacc gtcaccacat cccccacctt
      601 cagcacctcg cttgggtggc ggatgaatct gctgctgatc tggctgatat gcacaaggcc
      661 gtcctgatgc acgccgatgt ccacaaacgc gccgaaatca atgacattgc gcacggttcc
      721 cgtaagctgc atgcccggcg tcaggtcctt catgtccagc acgtcggtac gcaaaagggg
      781 cttcggcagg ctgtcccgca tgtcccggcc cgggcgcagc agctcttcta tcatatcctc
      841 cagcgtaggc acgcccacgc ccagctctgc ggccagcttc ccgtggccca ggcgctttgc
      901 ctgctgttcc atctcccgcg ccgccggcgt tccgatgtct gcgggcgaaa gcccgcacag
      961 cgccagcagc ttttccgccg cgccatagct ttccgggtgc acggccgttg cgtccagcgg
     1021 gtttttcgcg cccggcacac gcagaaaacc cgcgcactgc tcaaacgcct tggggccgag
     1081 cttcggcacc ttcagcagct cccgccgggt tttgaacgcg cccgcatcct cgcggtattt
     1141 gacgatgttc ttcgccgtgg ccgccgtcac gcctgccaca cgggcaagca gcggcgcgct
     1201 ggcagtgttc aggtccacac ccacagtgtt cacgcaggat tccaccacgc cgtccagcgc
     1261 ggccgaaagc tgcgccgggg gcatgtcgtg ctgatactgc ccaacaccca cggccttggg
     1321 gtcgatcttt acaagctcgg ccagcgggtc ctgcaggcgc cgggcaatgg ataccgcgct
     1381 gcgcaggttc acatcgtatt ccggaaactc ctcagccgcc agcttggagg ccgaatacac
     1441 gctggctccg gcctcgctca ccaccatata gctgacgggc aggtccagct ctgcgatcac
     1501 attcgccgca aatatctcgg tctccttgcc cgcggtaccg ttgccgatgg caatgatctc
     1561 cacaccgtgc tttttgatca gcgccttcag cgtacgcttc gcttcttcca cgcgcttaaa
     1621 ttccggaatc gggtaaatga cggcggtgtc cagcaccttg cccgtggcgt ccaccacagc
     1681 cgttttgcag cccatgcgat agccggggtc aagcccgagg gccaccttcc ccttgatggg
     1741 cggctgcagc aaaagctgcc gcaggttttt gctgaatacg ctgatggctc cctcgcaggc
     1801 gcgctccgtc aggcccgcgc gcacctcccg ctccaggctg gggtgtataa ggcgcacata
     1861 agcgtcctct gcggcggcgc gcacctgctc tgcgctgggg gaatcgttct tcaccgtgcg
     1921 ctcggtaagc agccgcacag cccgtgcggc gtccacggtg acgctcacct tcaggaagcc
     1981 ctcccgctcg cctctgtcca cggccagaat gcggtggccc gccatttttg cggcgggctc
     2041 gctgtaatcg taatagcccg aatatacgct gtctgtatct tttttcgcgg ccttgctctc
     2101 caccagtgcc gttgcgttgt acaggctgcg cagcgtagcg cgcagattgg ccgaatctga
     2161 aaagccctcg gccaaaatat cccgcgcgcc cgccagcgcc gcctctgtgt caggcacttc
     2221 ttcggttatg tactgccgtg ccagcagcgc gggttcgtcc tccggacgct gctccagcag
     2281 ccgcgccgca agcggctcca gcccccgtgc ccgggcgata gacgcccggg tcttgcgctt
     2341 gggcttatag gggcggtaga tgtcttcaac ctccgccagc gttttggcct tttccagcgc
     2401 ggcggcgatc tccggcgtca tcgcgccctg ctcggtaatg gaattgtgca cggtctcctt
     2461 ctggtcgtcc agcttgcgca gataggaaag ccgttcgtcc agcgcgcgca gcgtctggtc
     2521 gtccatactg cccgtggcct ccttgcggta ccgggcgatg aacgggatgg tgttgccctc
     2581 atcgatgagg gcgacggtgg ccgctacgcg cgcggcgtcc agcttgaatt cggctgcaag
     2641 agtgcttgcg tggttctgca tgtcaatgtt tctcctgttt ctttttcttt tccacaaaaa
     2701 cgataaagcc acagtagccc agccacacga ggatggcccc gagggtgacg cccgtggaga
     2761 gctttaggcc cggcgtttcg taggaaaata cgatggtgtt ttcgcccgcg gggcacagca
     2821 cggcgctcag gccgccatcc acgcgctcga tctcagcctc cacgccgttc accgtggcgg
     2881 taaagccctc gtcatacggc acagagaaga acaccagatt gtacttttcc agcgtgattt
     2941 cggccgtaaa gcctgtgccg tccgcggaaa agctggtgct ggccgtggcg cggcgggccg
     3001 ctgcgtcctg gacgtaggct tcgtaagtca ggttcgcagt gtcggctgtg gtggcttctt
     3061 ccatcagata accgtatttg gtgatctgct ctcccgtcag cgcaacggcg cgcatcagca
     3121 tattggatcg ggaatcctcc gccagcccca tcaatgtgac aggcgcgggc tcgcccgccc
     3181 gcagcgcgtc ctgcgccgcg cccgcctccg tgagcgcatc ggcggcgtcc accccctcct
     3241 ccgtctcggg gagcggggct tccagcagga tatatttgtc gtacgtaaag cccatgggca
     3301 catagttttc attgcggaaa tacaccagcg acgcgtcctc gtggtcatac acgtagccgt
     3361 acgcgcccca cttttcggtg aattcgctca tcttgtcctg aggcaccacc acatactcca
     3421 cgctcagcag accgcgcagt gcataaagct tctggtccgg cttggaggat acgtcccgct
     3481 tcacacccac acgggggtaa aaatccatga tggacggcgt gaccacgctg ttgaagaact
     3541 gaatgcacgg tttttccatc cacaggccga tgttatcgta acagtcgtag gaatcgattc
     3601 ggtaaaaatg atcgtcgggc aggtcgttat tacgggcggc cacataacac tcgctgcggt
     3661 agttcgcgtc gccctcccac tgcggaaatt tgcccagtgc aatatgcgcc accgaataga
     3721 ataccgaaaa gcccagaatt cctgccaaaa gcacacgggt gaatctctcg tgcccgcggc
     3781 accaatggat aacgccgtag aagatcagca ggcccaaagc agccgtcagg taggtcagcc
     3841 agaactggga ctgctccttc acgacgccca ggctccatac cccgtcctcc tgcttgggca
     3901 caagcgcgaa taccgcgaac agcagcgtta ccacggccac gggccgcagg ccctccatca
     3961 ggtcgatctc ctcgtcctgc agcgcctgca tggtggccgc gcacatgatg aggatgggca
     4021 tataatacca gcgtgcgtaa tagctggaat tgagcgcgta aaaggccgag ttcaacaccg
     4081 gcaccagcgc catgataagg cacgcccaca tgatttttgt aaacgcgctt ttcctgcgcg
     4141 ccttcatata cgcccacacg cccgcgcagc tcaccaccgg cagaaacgcc gtcatactgg
     4201 tgtgctttac caccgcgtcg gtaaagatat tgggcagata cgttgggtcc ggcgggagga
     4261 acagcgaggc cagaatggcg aaatactgct gcacgcggcc gtacagcaaa aagccgaagc
     4321 cgctggaaag gtccaccgtg cgcgggttgt ccttcaggca cagcacagcc ggccacagga
     4381 acaggcagcc cagcgccacg cccagcaggg attcaaacgc cagccgtcca aagcggcccg
     4441 gcgtgatcct gtattcgccc gtgagcagct tgatgaaaaa gtataaaaac aggaaaacaa
     4501 tttgaacggc aaaaaagaag taattgttga gcagattgac ggccaccgcc agcgggaata
     4561 cgccccgcac gccgtcgtaa acatagctgt ccagcgccca cagcaaaaac gggaacagcg
     4621 cgatgcagtc tacaaaatga ttgaaaaacg tattgtagac cgtaaaaccg gacagcgcat
     4681 acaggcatgc gccgatgaca gcatagttgc gtccggcgga ataccgcttc agata
//
LOCUS       DAWBSB010000172         4707 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_3179_length_4707_cov_3.159501, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000172 DAWBSB010000000
VERSION     DAWBSB010000172.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 4707)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4707)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4707
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3179_length_4707_cov_3.159501"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1031 a         1432 c         1308 g          936 t
ORIGIN      
        1 gcggtgtgtt gtaaaaaatt ccacgacctc cgggtccaca tgatagccgt tgtcctcgca
       61 ggccttttgc gccatgcgga tgccgccgta gggcatcagc gcacgcttga acggcttttc
      121 cgtctgaaag cccacgatgg tctctttctc cttgtccaaa tagcccgccg cgtgggaggt
      181 gatggtggag atgacgcggg tgtccatgtc cagcacgccg cccgcctcgc gctcggctct
      241 ggaaagctcc agcacctgct gccaaagcgc cttcgtgtcc tccgtcggcc cggcaaggaa
      301 cgcgtcgtcc ccgtcatagg gtgtataatt tttctggatg aagtcccgga cgtcgatttc
      361 tttttcccat acgccgggca caaagccggt ccattcgttt ctcatgcata gccctccgct
      421 tgtttctgcc gggccgcgcg gcacatccgc gttttccggt catgtctatt ataccatttt
      481 agtatagttt gtcaagacgc cgaccttccc cctgccggcg cgcggcaggg atgcttttct
      541 tgactttccg gatagagtat actatgattt ccccaaacgg tctgttgtta ttccaacaaa
      601 aggaggggcg ccatggactt tttatttctc tctcaaaccg cgctgttccg gggctgctcc
      661 ccccgggaaa ccgccgatat gctgcaatgc ctgcgcgccg agcgccgcgt ttacgagaag
      721 gaccgcgtca tttgccatgc gggggacacc gtggaagcgc tgggcctggt gctctccggc
      781 ggcgtcaaca tcgagagcgt ggacgtctgg ggcagccgga gcattctggg ccatgtgggc
      841 cccgggcagg tttttgccga aacctatgcc tgtctgccgg gcgaaccgct gatggtggac
      901 gccgtggcgg cccagcgcac cgaggtgctg tttttgaata cggcgcgcct gctgcgcacc
      961 tgtccctccg cctgcgccca ccatgcggcg ctcatccgca acctgctggc cgtgacatcg
     1021 cagaagaacc tcgcgctctc ccgccgcatt ttccacacct cgcccaaaac catccgcggg
     1081 cggctgctgt cctacctttc cgcccaggcg ctgcgggaaa acagccgcag cttcgccatt
     1141 cccttcgacc gccagcagct ggcggattac cttggcgtgg accgcagcgc gctttccgcc
     1201 gagctgggcc ggatgcgcgc ggaaggcctc atccgtttcc gcaaaaacga atttgagctg
     1261 ctgcgcgagc cctgagcacg gcttgcagtg gccgcaaaaa gaaagtacaa taatagaagc
     1321 aaagaaaggg gtggcgccat gagcaaaaag cgcgaggagc ttctggagca aatatgggcc
     1381 gcgcaggaca ccgcctatga cctgatgagc gaatacgatt ccctgcccca ttcctacggt
     1441 gaaaatgtgt tgtaccaggc ggaggcgcat ctcatcgacc ggatcgccct tcaccccggc
     1501 gtcaccgtca ccgaccttgc aaatatggtg cgcaaaacgc ccagcgcctg ctcccagctc
     1561 gtgcgcaggc tgcgggacaa gggctgggtg gaacaggtgc gcaacaccgc caacaaccgc
     1621 caggtaatgc tccgcctcac ccccgccggg gagcatgtat accgggacca cgccgccttc
     1681 gacaagcagt gccaggcgct gaccttccgg cgcctggagg ggttcaccga ggcggagctc
     1741 gccacctacc tctccatcca gcggcgcatc aacgaggcct atgcggacga cgtgcggcgc
     1801 agccgcgaat acttcacctg aaagcgcaca gcggcgcttc gtcagggtgt gcaaaaacac
     1861 gcgcggcttt tgttaaaaag gacgaaccat ataaaaaaga agcgctcccc cttgcacgcg
     1921 gccctgtgcg gtgttattat gaagctgctt aacatttaag ctgctttaca gcagtatttt
     1981 caacgcccgt tccggcgggt gaaaggagca ggagcatatg aaatacgatt ttaccagtat
     2041 catggaccgc cgcggcaaag acgccatcgc cgtggatgca ctgggcgcgc ccggcttcgc
     2101 acccggcgct ccgaaggagg gcttcgacgc cattcccatg tgggtggcgg atatgaactt
     2161 ccccactgtg cccaccattc ccgaggccat catcgcccgc gcccggcatc cggcctacgg
     2221 ctatttttct cccacggagg aatattttgc ttccatcatc cgctggcacg aaacgcgcaa
     2281 cggcgtcacc ggcctgacaa aggagtgcat cggctatgaa aacggcgtgc tgggcggcgt
     2341 tgtcagcgcg ctgaacattt tctgctcccg cggagacaat gttctgctgc actcccccac
     2401 ctacatcggt tttacccaca gcctggagaa caacggctac aacatcgtcc acagcccgct
     2461 ggtaaaagac gaaaacaatg tctggcgcat ggattttgag gacatggaaa aaaagatcgt
     2521 ggacaacagg gtccacgccg ccgtgttctg ctcgccccac aacccctgcg gccgcgtgtg
     2581 ggagcgctgg gagctggagc gcgccatgga gctgtacaaa aagcacgatg taatggtcgt
     2641 atccgacgag atctggtcgg acatcatcct cggcggacac aggcatatcc ccacgcagag
     2701 cgtcagcgag gacgcccgca gccgcaccgt tgccgtatac gccccctcca aaacgttcaa
     2761 cctggcgggc cttgtgggca gctaccacat tatttacaac cccgttctgc gggaccgcgt
     2821 ggagaaggaa gcctccctgt cccactacaa ttccatgaac gtgctctcca tgcacgcgct
     2881 cctcggcgcg taccggcccg aaggctacga gtgggtggac gagctgtgcc aggtgctgac
     2941 ggccaatgtg gatttcgcct gcgactttat cgcccgccgc tttgaaggcg tggaggtctc
     3001 caggccccag ggcacctaca tgctgtttct ggactgcacc cgctggtgcg aagcccacgg
     3061 caagagcatt gaagcgctgc agcgcgccgg ctgggacgtg ggcgtggcct ggcaggacgg
     3121 ccgtcccttc cacggtccgt gccacatccg catgaatctg gcattgccgc tctcccgggt
     3181 gcaggaggcc ttccgccgcc tggacgaata cgtgttcaac gcctgacggc cgcacttcgc
     3241 cgcatgcccg ccgggcggga taaagggccc ctcccgcggg gcagcaaaaa gcaattttta
     3301 aagggcagcc gttcaggctg ccctttataa ttatttgaat gccgcgcaga cgcttgctgt
     3361 tggatgcgga gcatggtata ttggcatccg cacagagaat tggagggaat aaaaatggac
     3421 caaaagcaaa gggcacggtt ggctatatta aaatcagtaa acgctcaaca tataaaagaa
     3481 tcggaatgga accggaacac tttattgcaa gaatgttttc attcgttaga aaggtatcaa
     3541 atccttaaag gtgaaaaaga gattgctcaa attgtaaaaa tcattaacac caatgacgca
     3601 aaacaaatga gccattgtga aaaaccaata cctctggaaa accgtgatta ttatgtagtt
     3661 tgggacagtg cacaattacc agtcattaag tgcaacggaa agagcattct atcaagttgg
     3721 gacgatgtag tggcagtcgc atttgatact cgcatcgttg atgcagaaaa agagcaaaca
     3781 tttttaatca gggggtagaa ccctggcttg ttcatggagc ataaaatatt tgtgtgcttt
     3841 tctaaacaat agcatgactt ctatcgcccg gaaaggcaat gagcccgcat aaaaaacaaa
     3901 atccctttgg gcgggaacac agccgctcaa tgcggcgcca aaaaagcgaa tgccggcagg
     3961 ttgcgcaaaa cgccgcccac cagcagcacg cccacggccc cccaaagcag cgccctttcc
     4021 caccggcgca cagagccgtc ccccgtgcgg acatagcgca cggcgtgcac cgcgccccaa
     4081 atgataccgg gaagcagcaa agcaagcaga acggcgtttg cccgcagcgc gcccggaaaa
     4141 tccagccgca gcagcgccag ccccatgcgg gacgcgccgc acagcgggca ccagagccca
     4201 agcgccgcat ggatggggca cgccgggccg cgccctgtaa ggccgaccca cagcgcatac
     4261 cccgcgcaca gcagcacggg cggcagcagc caccgggcca gccggcgcat gcgcgcacgc
     4321 tcctcttttt tcacccatgc acccctccgt tttccgcctg ttctgttccg attatagttt
     4381 cgtccgcccg gcggtgtcaa ggaggtgcgg agaaaacgaa aaaccatatt gacttttacc
     4441 gccgcagtat ggtaaaatat cattcgcggc acaaacgccg catggataag cgaggatggc
     4501 ggaatcggca gacgcgcacg tttgaggggc gtgtggttat accatgcgag ttcaagtctc
     4561 gttcctcgca ccaatttcaa aaaccgcatt gcaggctgat tttagcttta caatgcggtt
     4621 ttttcatttt ttgatttgtg ttatactcat ttatggtggt gtaatgacta agggattttc
     4681 agcatacgat tcagcactaa tgttctc
//
LOCUS       DAWBSB010000173         4644 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_3227_length_4644_cov_2.027457, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000173 DAWBSB010000000
VERSION     DAWBSB010000173.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 4644)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4644)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4644
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3227_length_4644_cov_2.027457"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2886..2895
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3816..3825
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4220..4229
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1037 a         1351 c         1110 g         1116 t
ORIGIN      
        1 cctcctttct gaaagagcgc ccccttgacc ggggtcgctt ttgattttga ttcaatgccg
       61 cggtttattg aacacggctg ttgtaacgtt ccagtgctgt tgtctgaagc tgcagcacgg
      121 tatcaaggcc gagtgccttc aggttgtcct gatatttcgc aaagttatcc agagattcgt
      181 tgcccataac aaacttcgag aacatctctg ccgcataggt ggaaagctcg ttgtaagcat
      241 tggcatactc cgccgattcc tcggtgagca tcgtcgtgga gggcagcccg gcgcggaccg
      301 tgccgttcgc actggaggac cacagatcaa gcgatttcag gttttcgtct acttcgcctt
      361 cctgcagcgt ataatcacgt ttggattcca agcgctggga gtgatccatc ggatacgggc
      421 cattcaggtt gcggtaaacg gcgaacgctt cgctgggggc aatgccctga tcgttgttga
      481 cgatgctgtc cgtcagcttc ggccagccgt ccacgaattc atagctttcg ccttcgatgc
      541 cccagttcag gatacggttg ccttcctcgc tccagccgta atccagtacc ctacacgcgg
      601 tggcaatatc ctcacaggaa gtggtgatgg ccgcgcccga accaatgtag gcagcgttgc
      661 cctgcttgta gacgagctgc tgcccctctt cgagcacggg gttcagcacg cccacagggt
      721 aaaactcaga attggggtcg tcagccagca cctgacggcg cagattgccc aggccggcag
      781 gcatcatttc aacccaagcg cccacttcac cattgatgac cttggattgt acgttcgtac
      841 ggtcctgcac catgaaatcc gggtccagca gaccttcgga atagagcttg ttcataaaag
      901 caagatactc cttgtagctg tcctgtgccg ggccatagaa gatggagccg tcgtccttca
      961 gcgcaaatgc tgtgtttccg atgtcatatc ccacgaccgg caccgtgata tcgtaagccg
     1021 aagagaagca ctgagccatc gtcagcatca tggcctggca ggaaagagga tatttgatgc
     1081 ccatatcctt aaacgccacc agcgcttcat accaatcgtc cagcgtgaca ggaatatcca
     1141 gccccgcttt atccagcagg tccttgcgga ccaccaggcc gatgaagctt tcttcatatg
     1201 gttcacggtc aatggtgccc tgccacacat cgctgtcctg gctcgtatag gtataaaccc
     1261 cagggaagca gtagatatta ccggagtctg tggtgatctc ctgccggacg tccggatgcg
     1321 cgtccagata tgcggaaaaa ttgggggcat attcttcaat gtaatcgttc aacgccacga
     1381 tcttcccatc cgcgattgcc gcatccgggc cgccgggata agacgtagcc caactgtaat
     1441 acataatgtc gggcaggtcg ctggtcgcca cgatcaggtt gaactgctca gcagactggc
     1501 cgctggccgg ctcctgccaa tcaatgtcca cgcccacatt tttctcatag agctgccagc
     1561 ccttgtgttc cgaaaaggat ttcacttgat tggacgccat gttttgcggg tccatccacc
     1621 aggatacggt tgttgccgtc ccggcggtgc tgccgcctgc gctggaagca gagcctgccg
     1681 cgggcgcgga agagcttgcg cttccaccgc agccggccag cgagagcgcc atgactgcac
     1741 atgcgaggaa tgcggaaatg atcttcttca tgtttgtacc tcttttcact ccattttttg
     1801 ttatcccgaa caattcgggg tttcaacgct ttcagcctaa cagtaatccg tcaaaattgc
     1861 tatctttagt ttttaatgca atgtattttt gttttgtatt tacagttttt cgccatcgct
     1921 atttttttgt ctatcactat ttttttcaaa tctatttgta aaccgcacaa aaaaagcggg
     1981 aaagttttgt caaccttccc gccgagacga tatttcaagc tcagctgttg atgcttccgg
     2041 gcgtcgtgcc ctcatatttt ttgaagatac ggataaacgt attgatattt tcgaacccgg
     2101 cctcatgcgc ggcttgcgcg accgaaatgt gccgttccaa aatacactgc ttcgcatatt
     2161 tgacgcgcag gccgctgatg taattcaaaa ggccgatccc tgtttgctgt ttgaacaggt
     2221 tggaaagata cggtacgctc atgcccaggt attccgcggc ccgggataca ttgaaatcca
     2281 ggtctctgta atgctcattc acacactgca tgatgcgctc catcggctgt tcccgcgctg
     2341 cgcccgccac agtgtgaaag ccctcacagg cggcggatga gcgaagcacc gtcgccatca
     2401 gcatctgtgc tatctcatcc cgcgtgcctg cgtggcgcag cagatgcagg tccgcccgca
     2461 gcgcctcccg ctgctgctcc cacacctcgg gcatggttcc caggcagcgc agaataccgc
     2521 acaacaggtc atacgccaaa cagcggcaca tgctgacagg gagctcctgc tcttcccaat
     2581 tgtgccgcag gatcatctgc agaacagaag ctgcttcttc ttcatttcct gccgcgatgt
     2641 agcgcatcaa aagctcttcc tgctcactgg aaagccgcgg cacctgcgcc ggctgcagag
     2701 tcggcccgca ttgttcctga caaccggcgg ctttttcatg ctgttcgcgc agcgcttcgt
     2761 tggccgccag ataagcaaga tggatgtttt taaaatcccg ccatggtttt gaaaaggcgc
     2821 acaaaaaatc tccgcccgcc tcccgcgccg cgcgtttcag caaagcgtcc agctccgcca
     2881 cggttnnnnn nnnnngcgcc atatggttca aaagatcctc cagcgcttca cgctcactgc
     2941 cgctgtccag cagcgtctcc gctccgcctc cggcgtccag caggaccaca ccgaaccaat
     3001 cgccgctgaa gccggcatcc agacgctcca ggacctgatg cagatgcttt tcatcatata
     3061 caacgtcgcc ttcaatgcag gactgcacaa attcctgccg cagctcgttc ctctggttct
     3121 cccacagcat ttgtatctga tctctgctgc tttttgcgtc tgtaaacgca gccaacagct
     3181 ggtcatactc atttccgctc agcgcgtggc ccgctgtgcc ggcaagttcc ttgcgaagcc
     3241 ggttgagcgg cttgtattgg atatacaaaa aacacagaca cagcaccgct gtcatgcaga
     3301 ccatagccag catgaccggg agtgcccggc ggatgatcca atctgacttt gcaccgatgg
     3361 aagacatcgg tacgaccgtg ataagatgcc acccggcttt cgggagccgt tcgcgctgta
     3421 tccagtagag ttgtccgccc aatgtgattt ccgagtcctc cgcggaagac acgtcggcat
     3481 tttccagcag ctccgcaacc gcagcgtcac ctgttgcgct cagcaatgag ccgtccgaat
     3541 ccagcagcac tgtcgtactg tcctcaacag cggagtttga ctggatcatt cccgcaatgg
     3601 tttcggggtt catcacttgt accaatgttc cttttggcgt tccgcccaca aggggcacgc
     3661 tcgtcagcat caatgcttct gattttccac ctgtttcaaa cacatacagc tcgttgtaat
     3721 gatagtcctg caaaagtgtg gtgaaatccc cggcagacat ggcggcttcc agccccaacg
     3781 aagcataaaa ttcatgcagc ctgaaaatgg cgtcannnnn nnnnncctct ccgccggact
     3841 ggagcgcgta taccgaaaga gactgctgaa tgggctgcaa attgtaatat ttcgagccgc
     3901 ttgagtcagg cgtagaaaca aaatcgttta ccagataatc gttgctgatc tcatacgcac
     3961 agcgctccag ttcatcgaaa taatcattga tcgcgttgtt gctctgcttt tgcagcgtct
     4021 gcgtaataga tttatattca ctgatgagct ggtttcgcgc attgcgcccc agcataatgc
     4081 acatgaccac catcatcaag agcagcagtc cataggaaag acaccaggta tacaatacgc
     4141 tttttcgttt aaacatcttc cccatccctc tcgttagcca tagaaattat tatagcacac
     4201 atgcagacgg cgcgcaaaan nnnnnnnnnc gcgcaaaata tcgaaacaaa agagactgcg
     4261 gacgccctct cttcgatagc gcccgcagcc ccaatatatc cttaataaat agcagcaata
     4321 tgtatatgcc gtacagcctg agagaaaaag cttttattct tcgccacacg cggggaaccg
     4381 cacgcaagca tatcctcaac cacaccttcg aaatgcacag aggcgacacg ccggcggttc
     4441 atgcaccgcg ccatcaactt tacatgatgt ttgtttgtgg agatatcttc gattttccgt
     4501 cggaccccac cgtctttcca aagtgcgatt ccataggtcc tatgaaccac accgtgctca
     4561 tctcttatcg ctcctgctgt tacaaaataa tactccaact ggaatccgcc ctttccgccg
     4621 aaaataataa ttgcagaatc cagt
//
LOCUS       DAWBSB010000174         4562 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_3280_length_4562_cov_3.623918, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000174 DAWBSB010000000
VERSION     DAWBSB010000174.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 4562)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4562)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4562
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3280_length_4562_cov_3.623918"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          794 a         1307 c         1561 g          900 t
ORIGIN      
        1 cggctgtaag ctttgcgggc ctactgggtt ttgtggggct tgttgcgccg catatggtgc
       61 ggcggctggt gggcggtgaa agcaggcgcc tgctggcggg ctgcgcgctg ggcggcgcgg
      121 cgctgctcac cgtgtgtgac gtggccgcgc gcacgctgtt cgcgccgtac gagctgccgg
      181 tgggcgtcgt gctctccctc gcgggcgggc cgttcttcct ctggctgctg ttccgcaaag
      241 ggaggggaca tccatgatcg agctgcgtaa aataagcgcc gggtaccgcg gagagccggt
      301 gctgcgggac gtggaccttg tgttccccgc gggctgcgtg acggtgctgc tggggccgaa
      361 cggctgcggc aaaagcacgc tgctgaaaac ggcgctgggg ctgctgcccg cgctttcggg
      421 cgaggtgctg tacgacggcg cgccgctgtc cggcatgccg cccgagcagg tggcgcggcg
      481 cgcggcgtat atggcccaga gccgcaatgt gcccagcatc gaggcgcggc gcatggtgct
      541 gcacgggcgg ttcccgtacc tgtcgttccc gcggcgctac cgcaaaagcg actatgcggc
      601 cgtgcggcgc gcgatggaaa aggcggacgc gctggagctg gcggaccgtc cgatgcagga
      661 gctgagcggc ggccagcggc agaaggtgta tctggccatg gcgctggccc aggagacgcc
      721 ggccgtcttt atggacgagc ctactacttt cctcgacgtg cgccaccaga tggatgtgat
      781 gcgcacggcg cgcggccttg cggacggggg aaaggccgtc gtgctggtga gccatgacct
      841 gtgccaggcg ctgcgcaccg ccgaccgcgt ggcgctgctg gcggacggaa ggctgtgcat
      901 ggcgggcacg ccggagcagg tgtacgccgg cggcgcgctg gaccgggtgt tcggcgtgcg
      961 ggtgcggcgc gtgcccgtgg acggcggctg gcagtatttt tgcgaatgaa ccggacaggc
     1021 gcgggccgga cggccggtat ctccgcctga atttgtcgtg atgggagcgc agtgtatggc
     1081 gtattttccg atgtttgtgg agctggaggg acggccctgc ctgatcgtgg gcggcggcgc
     1141 ggtcgcgctg cgcaaggcgc gcaagctgct gccctatggc ccgtgcctga cggtggttgc
     1201 gcagagcttt gtgccggagc tggaagcact ggagggagct gcgctgtgcc gccgggcatt
     1261 tcgcccccgg gacgtggagg gacaggcgct ggtggtcgcc gcgacgggcg acggcgcgct
     1321 gaaccgggag atcgccgcgc tgtgccgggc gcggcgcatc cctgtgaacg cggtggacga
     1381 caaggacaac tgtaccttcc tgttcccggc gctggtgcgg cgcgggccgc tttccatcgg
     1441 catctccaca ggcggggcca gccccacggc cgcggtatat gtaaaggaaa aaatcgaagc
     1501 ggcgctgccc ggcagcgacg gctggaacgg gatattggaa tatctggccg cccggcgcgc
     1561 ccctgttcgg gcctcggtgc cggacgaaac cgcccgggcg cggctgttcg cggcgctgtt
     1621 cgacgcatgt atggaaaagg gaaggccgct ggagcaggcc gaattcgacg cgctggcggc
     1681 gcggatggaa agagaggggc cgggggatgc ataaaaaggg ctttgtctat ctggtgggcg
     1741 cgggctgcgg cagcgccgac ctcatcacgg tgcgggggct gcgtctgctg caaagctgcg
     1801 acgcggtggt ttacgacgac ctcatcgatc cggccctgct ggcgcaggcc gcgcacgcgg
     1861 agcagcatcc cgcaggcaag cggtgcggca ggcattccat gccgcaaagc gagatcaaca
     1921 gcctgctggt gcggctgggc caaagcggaa agaccgtcgt gcgcctgaag ggcggcgacc
     1981 cgttcgtgtt cggccggggc ggggaggaat ttctggcgct gcaggcggcg ggcgtcccct
     2041 gtgaggaagt gccggggatt tcttcgtgca tcgcggttcc ggccgcagcg ggcattcccg
     2101 tcacacaccg gggggcaagc cgcagcttcc atgtcattac gggccacacg gcacaaacgg
     2161 gcgacacgct gccggaatct ctggaggcgc tggcggcgct gcacggcacg cttgtgtttt
     2221 tgatggggct gaacagcctg gaacagatcg ccgcgcggct gacggctgcg ggcaagccgc
     2281 cggaaacgcc cgcggcggtg gtgtccggcg gaaatgcacc gcacccggcc acggtgcgcg
     2341 gcacgcttgc ggacattgcc gaaaaggcgc gcgcagcccg ggtgcaggcg cccgccgtca
     2401 tcgtggtggg cgccgccgcg gcgctggagc tttgcgcccc ggccgcgccc gtgctgccgc
     2461 tggcaggcgt gcgcgtgggg ctggtgggaa cgcccgcctt cacagaacgg ctcgcccggc
     2521 gtttggaagc actgggcgcg gccgcggttc cctgcatgac ggcccgggtg cggcctttgc
     2581 cgccccagcc gggctgggaa aggctggcgg acgggcgctc ctgctgggtg gtgctgacga
     2641 gccgcaacgg cgtcggctgc ttttttgacg cgcttgccgc ggcgcgcatc gacgtgcgcc
     2701 gtctgcacgc ctgccgtttt gccgccatcg gcccggctac ggcggaggcg ctggcacaga
     2761 ggggcatcac ggcggatctg tgcccggagc aggccacagg cgcggaactg gcccgtgcgc
     2821 tgtgcgccgc cgccggcccc ggcgaagaga tcctgctgtt tcgcgccgcg gaaagcagcc
     2881 cggagatgcg cggcatcctg acggctcaag gcttttcggt tcgggaatat acgctgtaca
     2941 aaacggaata tgccgcggca tcgcccaatg cggaggcaga ctatctggcg tttgccagcg
     3001 cgggcggcgt gcgggcatgg ttctccgcct gcggcgcggc gccgaagggc gctgtgtgcg
     3061 tgtgcatcgg cccggtgacg gcccgcgcgc tggcgcagca gaccggtgcg ccgttcctga
     3121 cggcggagga catctccgca gagggcgtgg ccgaatgcat tctgcgcgca caccggcaca
     3181 agaaagcgca ggaggagtaa aacgccccgc cgcacaggcg gcaggacagg aggcctgaat
     3241 tcatatggga tgtattattc ccggcttgcg ggaccgtgat ttgaagaaaa cagggataag
     3301 ccgatacggg tttatttcca cagcggagat cctgttcagc gatgcggttc ggaagatctg
     3361 cgaaaacgat acctgccgct tgtacggaag aacctgggcg tgccctcccg cggttggaac
     3421 ggttgaacag tgcaggcagc ggtgcctccg gtatgaaaag gcgatggttt ttgacgccgt
     3481 atatcctttg acagaccctt ttgattacga aggaatgacc gaaggacacg gggcgtttaa
     3541 aaagctgtgt gacaggctgt atatccttgt gaaaaaccag ctttcttcat tccttctttt
     3601 gtccaatgaa ggctgtaaca gatgtgaagc ctgcacctat ccggagggtg catgcagaat
     3661 gccggaaatg ctgtttccct ctttggaagg cttcgggatc gatgttgcaa gcctggcggc
     3721 aaaagccggc ctgacctata acggcggaga aaatacagtg acttactttg ggatgctgct
     3781 gtattgatct ggcacgtttt tgctttccgt ccgccggata caaaaaacct cctgccggga
     3841 atcccctcgt aaggaagttc cttagaaaat gcatgaatca atgaagtccg tacccttttc
     3901 agggtgcgga cttctacttt ggactgcagt gccgcaatcc gatgctcgtt atacctgtgg
     3961 acacgctcac accagcacag tcgccatatg ctgttttttt gataaacgga aaattgtcct
     4021 attcaagcca ttgaataaaa gcggttttat cactgctgtt ataccctgcg cttctataaa
     4081 acttaagtat tgactcttcc tttgaaccgg tgagcagcat cattttataa caattttcca
     4141 ttttggcaat ttctttggca taattcaaac attctgttgc ataacctctg ccgcggcagt
     4201 cttttcgggt aacaacattt tctataaagg cgtacggccg gacatttctt gttaaattag
     4261 gaattattac gcaaacacaa gaggatacga ttttattatc agcctcattc actataatgt
     4321 gatgattttt atcttttata atcgtttccc atgtcgtttt taaatgttcc gtcctttccg
     4381 gtacagactt ctcgtgcaga tatagataca gatttaacaa tttacttagc tcgttttcgt
     4441 aaatttctct aaccatacag cacctctaca aagtctatac gtccttacga agtgtcctcc
     4501 ccccggcagg agtttttttg taagaaaggc ctttgctgag ggaaggcggc cctccctcaa
     4561 aa
//
LOCUS       DAWBSB010000175         4545 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_3290_length_4545_cov_3.420045, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000175 DAWBSB010000000
VERSION     DAWBSB010000175.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 4545)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4545)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4545
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3290_length_4545_cov_3.420045"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          990 a         1439 c         1356 g          760 t
ORIGIN      
        1 gtcgtagctt ccgtctgttc tgcggtaacg gtcgcacagc tcccgcagcc ggcgctcccg
       61 gtcctcccag tcgattctgc ccaccttgtt gcggcaggcg tggcaggcgt cacacacacc
      121 gtcctccccg atgtgcatgc tttttttcac gctgttgatg gtatgctcga actcatagca
      181 tgaattgggc ttctggttgc tcatcacaca ttccctgcag aactgcacat gggcaggcag
      241 accgtatcgg gcctccagct ccgcctcaga caggtccggc gcgaattttg cataatcgat
      301 gggaacatag cgtttatacg gttccatact tcatttcccc ttatcctcat attccttgct
      361 gtatccggca gcaaaaaagg cgccgcccgc agggtatacc ataccctgcg ggcagcgcct
      421 ttgctgttca cacgcattat cataccacag tgcccggcag ctttcaagcc tgcggcccat
      481 tttatccgaa ttgtacattt gcccgaaaaa agtatataat agggaataac aaaacaacga
      541 agggcgccga cggccgcgcc ccccgaaagg aacgactata tggaaaacga cggaaaggtc
      601 atcgcgctgc tcatcgacgc ggagaacgtc tcgcccaaat acatcaagac gatcatcgac
      661 gaggtgagca tgtacggcac ccccgcctac aagcgcattt acggagattg gacggccccc
      721 gacatggtga gctggaaaaa aatgcttctg gagcacaacc tgacgcccat ccagcagttc
      781 agctacacac agggcaaaaa cgcgtcggac tccgccatga tcatcgacgc aatggatatt
      841 ctgtacacaa aaaatgtgga cgggttctgc atcgtcagct cagattccga cttcacgcgg
      901 ctggccgcgc gcctgcgcga gagccgtatg ttcgtcattg gcatgggaga gagcaagacc
      961 cccacggcgt tcaaatccgc ctgcgacacc ttcaaatacc tggacgtgct gatgggaatg
     1021 aaaaaggaga cgctgcccgt ggctgtgtcc gaaaaggctt ccaagcagcc ggcccagcag
     1081 ccgaaagccg cgaagaagca ggcccagccc gctcagccaa aaaaagccgc aggcaaaaag
     1141 ggtcagaagc cggagccgga aaaggaaccg gagcccgttc ccgccgcgcc tgcggagccg
     1201 catcacgacg aggagctggc cgccgcgttc gtctctttgg acgagatcgt agcaaaaatc
     1261 cgcgagatcg tagagcacga ctccgacgag gacggctata tgccgctttc ccaggtgggc
     1321 gtgatgctct cgcgtattta cgcggacttt gattcccgca actacggctt ctccaagctg
     1381 cacaagctca tcgactatac caaagcgttc gagacgaagt acgagcagtt tgaaaacggc
     1441 ggaaagaacc tcgttatccg caacaagggg tgaacgggat ggcgccggag ctggagcggc
     1501 agcttgccgc ataccatccc tgctgcgagc aggaagccgt ggaccgggac gtgatgctgc
     1561 ggtttctgcg ggaaaacacg gatgcgttca cgcgggaaaa ccttgtggcg catttcaccg
     1621 catcgtcgtg gatcgtaaac agcagccgta cccatgcgct gatggcctgg cacaacctgt
     1681 accgggcatg gagctggacg ggcggccacg ccgacggcga aacagacctg ctttccgtgg
     1741 ctctgcggga ggcccgggag gagacgggcc tcgttcacgt cgcgcccgta tttccggaga
     1801 tcttctcact ggagaccctc tgggtggagg ggcacgaaaa gcgcggcaaa tatgtgccgt
     1861 gtcatctgca tctgaacgta acctatctgc tggaagccga cgcaggagac gcgctgcggg
     1921 caaagcccga cgagaacagc ggcgtgcgct ggttcatgct ggacgaagcc gtggaggcct
     1981 ccaccgagga atggatgcgc gcccgcgtgt acgaaaagct gaaccgcaag gtgcgcgcgc
     2041 tgggctgacg cccgcaagtc tttgaaaaag ctgccgcgtc cggggcgggg cggggcggag
     2101 gatagccctt ccgcccgccg ggcgcttttt gaggaaaact ctatgaagat cgaccgtctc
     2161 atcggcatcg tcacgatcct gctgcaaacc gaaaaaagca ccgtgcccgc gctggcgcag
     2221 cggtttgagg tgtcgcgccg caccatcgag cgcgacatcg acgcactgtg ccgggccggt
     2281 gtcccgctgg taacggtgca gggctacggc ggcggggtat tcattgaacc gcgctataag
     2341 ctggacaaga cgctgctctc cggcccggag ctggccgctc tggtggccgg agctgcgggc
     2401 gtaggcagcg tttcccccgc gccgctggcc cgcagcctgg ccgaaaagct gggcggttca
     2461 ggcgcggacg acctgctcat cgacctctcc tcccattacc gggagagcct gacaccgaag
     2521 atagagctgc ttcgcacggc catcgcagag catgcccgtg tgcgcttcac ctattactat
     2581 gccaaaggcg ttgcagaaaa atgtgcggag ccctaccgca tcgtatttca gtggggagac
     2641 tggtacatgt tcggctggtg tccggacgcg gcaggctggc gcatgtacaa gcttaaccgc
     2701 ctgacgctcc tgtccgtcac ggcgcagacg tttgaacccc gcgccatacc gcccgaagcg
     2761 ctggatttca acgcgcgctt ccacgacggc acactgctgc gcgcccgcgt cgcggccagc
     2821 gaatgctacc gcctcgtgga ggagtacggc ccggcctgct atacccgcca gccggacgga
     2881 agcctgctgc tggagatcgg ctacaccaac gaagacgtcg cggcagcctg gctgctgggg
     2941 ttcggggcaa aggccgaagt gctggaaccg cccgcgctgc gggcgcgcat ggcgtccgag
     3001 gcgcaggctc tgtgcgcggt atatggcgcg ccaaaaaaat aaaaacacag ggacacgaca
     3061 cgcagatgtc gtgtccctgt gtttataatc aatgcaaagg aggcgaaccg ccatgaaaaa
     3121 aattgagacc ggggccgcac ggcccgaaac gtttcccgag caatggccgg gacaatgtga
     3181 ggtgttttcc catttggaat acgcctgcgg catcccgtcc atcctgtacg ccgttacaac
     3241 gtataaagaa aacggcctgg gcaatgtatg ctgcggcgca tgggccagct ttcccggaga
     3301 cggcggcggc ttttatgcgc tggtgccgct gccggccagc acgcacacac tggcgaatat
     3361 ccgccgcacc ggagaattct gcgtcaactt tcttcccgtc cggcactttg acgcgctgat
     3421 gcgcaccgtg cgccaaaacg gcatggagga cgatgagttt accgagggcg gcttcacgcc
     3481 ggagcccgcc cgcactgtgg gtgctccgcg cattgcggag agctttctca cgctggaatg
     3541 cagagcagaa cgcatcgaag ctctttccga ttcgggacgc atgatgttgg tgaaagggcg
     3601 ggtactcaac gcggtatccg cgccggatca cgcacagggc gtggacaaac gatacggtcc
     3661 ggagggcttt gtcctcaaca tcaacgagcc ccggaacctg ctggcgcatc attcggacgc
     3721 ccccagtgcc ctggccacac tgcatgtgga gcgggaatat caataacaga caaaatcaca
     3781 aacgcccggc ggaacactcc gccgggcgct tgtgctgtta taaaatcatc cccgcctggc
     3841 aatgttccgc gcgcaagccg cgcccagcag gcccgtgcat cccagcgtga tggccagcgg
     3901 aaacgagacc ggcgtgtcca cgccgttcat cccctgccat acggcctggg gcagtgcaag
     3961 gcaggtgcag aagaagaaag ccagcagccc gcagcggctg agtgccggcg ccgtgccgac
     4021 gccgccgggc agatacagcg cccgcagcag cgccgtcaca aacagaagcg ccgccagcgc
     4081 ggaaaaaatc tccaccgccg gcaggatgcg gtacaggctg gcgggctgct ccacaaaacg
     4141 caccactgtg tgcaggtaaa acgcggcgct tccggccacg cccagataca gcatccagcc
     4201 gccccgtttt tcccgggcgc ggttttcaaa aaacaccaga aggcaccaca cgccgaacac
     4261 gacgaacagc gccgcgcgcg tcacatccgc cagctgcccc agcaggatgc gctccgccgt
     4321 ctggtggcgg cccgcctgct ccggcgtgcc gaacagcacg ttcagcaccg tcaaaaagcc
     4381 gtacagctca ctgcagaagg ccagcggcac aaacacaaaa ttcacgccgc tcatccacac
     4441 gcccgcaccc ggccgcagcc cgcagggctg cttgcgcggc acatacagcc cggccagcca
     4501 cgccagaatg acaacaggcg ccatcagcca gtaccgggtc cagac
//
LOCUS       DAWBSB010000176         4531 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_3301_length_4531_cov_3.235478, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000176 DAWBSB010000000
VERSION     DAWBSB010000176.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 4531)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4531)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4531
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3301_length_4531_cov_3.235478"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1830..1839
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4019..4028
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          900 a         1301 c         1233 g         1077 t
ORIGIN      
        1 cgggacagca ggtccatttt tccgctgata taagcggctc ccgccgcgcc ggccaaaaat
       61 acaaccagca cgacccccgc cgttaccgat aagaatatct tttttctgcg tgaaaagctg
      121 tgcttcactg taatgcctcc ccgcgccggg cgcgttgttt cctttgtaga ctgatactag
      181 cacagaaata taaaaaattt gtggaatttc cggataaaat ttttccgcaa ggtaccgaag
      241 agcccgaaag tcctccattt gtcttttgcc atttatttta tattccccgc attttggatt
      301 gatttcacaa atttttgttt tataattaaa ttaatttagc gttttagcat caatataaag
      361 gaggccgttt atggaaaatc ccgcgtttga cgtcgcggcg ctgcttgcgc cgccggatat
      421 cctgaaaagc aaatgtgctc tctgcatcca gccccatccc gacgacaacg agatcggcat
      481 gggcggcacc gtggcggtgt tggcgcaggc gggctgcacg gtgcattacc tcaccgtcac
      541 caacggcgac caagggaata agaaccgcac ggcgtccccg caggagaccg cacgtgtgcg
      601 ccgcgccgaa gccattgccg ccggaacaca cctcggtgca aaggagttcc actttctgga
      661 ccacggcgac ggcacgctgg acgatgtgct gggcctttcg gcacagatcg cccgggtcat
      721 ccgcgaggtg cggccggacg ctattttcgc gccggacccc tggctggcct acgagagcca
      781 cctggaccat gctgtaacag ggcgcgcggc agccaacgcc tttttgatga gcggccgcgc
      841 cgccattccc ggcggcgggg acacgcagcc gcacgccgcg tctgccatcg gctattattt
      901 taccgcgcag cccaataccg ttgtggatat ttccgccgta ttcgaaaaaa agttcgaggc
      961 catcgcgctg cacgacagcc agatggatgc gcagacgttg gccatgtatc gcgtctactt
     1021 cggcatgaag ggaaaagaac tggccgcggg taagaacttt gcgctgggcg aggggctgaa
     1081 agttctctct ccgctgcacg cgcactgctt tgtggatgcc gcgcgcatct gaatacgatc
     1141 gaaagaggcg agaatatgga aaaaaacaag acctgcaacc gcccctggcg ctattctctg
     1201 gcgatgttcg gtttgtccat cgcaggatac atgtacgtta cttacggcac ctttttttac
     1261 accgagacca tcggcctttc catgaagctg atcgcgctgg gcaatgtgct gtatgcggtt
     1321 tgggacgctt tcaacgaccc catcgcgggt tatctgtcgg accgcacacg cacccgctgg
     1381 ggacggcgca aaccctggct gctggtgagc gtgcctgtgt tcgtgctttc gtccatcctg
     1441 tttttcagcc cgcccgcatc cctgggcacg ggcgtggggc tggccgtata tttcacggta
     1501 tttctgatgc tgacagagac gggcaacacc atctccaccg tgaactacca ctccctgctg
     1561 cccgagctgt accgcgatga aaagcgccgc aaccaggcga acgccctgcg gcaggccatg
     1621 cagctggtgg gcatgatcat cggcgtttcg ctggtgccca tgatcactgc ggccatcggt
     1681 taccgcgcca cggcgctggc gctggggctg ctcagcggcg cgctcatcct ttattccata
     1741 ctggggtgcc gggagcgcag tgaattcagc caaagcgaac agcccggcct gctggcttcg
     1801 ttgaaagctc tggccaccaa cgccaatttn nnnnnnnnnt ttctggcttg tgtccgtctc
     1861 ccactttttc taccagtcca ccagcgggct gttgctggcg ggcattccgt tctacatcaa
     1921 gtacgcgctg cagctgccgg acagcaacgc cacatttctc tccgcggccg tgttcgtcag
     1981 cgccatcccc tcgatgtacc tctggtaccg ccttatcaac cggctgggca cgcttaaatg
     2041 ctggcgcatc gcgctggcgt ggctgggact ttcgctggtg ccgatgtact ttgtgcacag
     2101 cctgctgccc gcatgccttg ccggcgtatt ggtgggtgtg ggcatcgcgg gcgtgaccgc
     2161 caacctggac atggtgaatt ccgtgctcat cgaggacgac gcggcccgct atggcgtgcg
     2221 ccgcgaggcc acgttcttcg cgggcatcag ctttatcacc cgtctttcca gcctgacgcg
     2281 cagcggcgtt tttcttctgc tgttcacgct gttcggcttt gaatccggcg aaaaccccgg
     2341 cgcaatgccc ggcactgccg cccgctttat gatgatcgtc tttcccatcg cgctgatggc
     2401 gctctctgtg tgcgcctctc tgcttgtgcg cttccgcgct cccgccgcca gggccgcaaa
     2461 gcccgcggca aagctgtaga tccctccgcc gcccggcgct tctgcgcatt tccgggcggc
     2521 gctttttctt accgctcttt tctcctgcgg ggaaatatgg tacaatgccc cgcagaagag
     2581 gcagcggcgc acagggccgc cggggaaagg ggaaatggag catggactat acgctcgtca
     2641 agcaggtgcg ggacaatgct cccctgcggg agagtttttt cgcactggcc cggcgtgtgt
     2701 tcggcctttc gttcgaggaa tggcaccgcg cgggctggtg gacggaccgc tacgttccct
     2761 atacgatggt ataccgggac ggcagcgcgg cagccaacgt ctcggtcaac cgcatcgaca
     2821 cggtgtggcg tggcgaggcg ctgcggctgg tgcagctggg cacggtgatg acggctccgg
     2881 agcaccgcgg caagggcctt gcccgcagtc tgatggaagc cgtactggac gactggggcc
     2941 gcacctgcga cggcgtttat ctttatgcca acggcacggt gctggagttt taccccaaat
     3001 tcggcttcgt ccccgcgcgg gagcatgtct gcacactgcc gctgcccgcc cggggcgccg
     3061 ctccggcgga gggcggtttt gcgcggctgg acatggacac gcaggccggg cgcgcgctgc
     3121 tgctgcgctg ctatgagcgc tccaaccctt tctccgctct gcccatgctg gataaccccg
     3181 gcctgctgat gttctattgc agcggctttt tgaaggactg cgtctattac tcttccgctt
     3241 atgatctggc ctgtgtggcc acggaggagg acggcgtttt cacctgctgc gactttttcg
     3301 gcacgggccg ctgcacgctg gagcaggcag tgtgcgccgc tgcctctgcc gcttcgcccg
     3361 gcgcggcgcg ggccgaactg ggcttcacgc ccgcaagcgc cgccgcatac gcccgcgcgc
     3421 ccgtgcaggg cgacgaccat ttgttcgtgc tggcgggcgg agcgaatatt tttgcggaag
     3481 accgcgtgat gctgccgctg ctttcccatg cctgaacgcg gcgccgcaaa acgccagaga
     3541 agagaggagg aggctaaatg ccagcgctac atagggaagt aatttggcag gtcaatgcaa
     3601 tatcaagagt tcttaaaaaa tcctatacag aaagccgcct gtatatatta cacaggcggc
     3661 ttttggttta atgtcatact atatattgta tttgtagcag tgttttaccc tttgcatttt
     3721 gtcctgtcgc ttaaatccgc tgtacaatat taaaagctat caagatattc ttgcggttaa
     3781 tagatcattc gttaattagt tcaacaatcc agtttatcca gcctttaata gtggatgaac
     3841 gtcgttcata tgtactactg ctattcacat tatacaaatt ggattttttc atgatttgta
     3901 taatttcatt tttgttcggc atagtcccgg tctcaaaata tctttttagg gtatcagcaa
     3961 acacaccatg cgataaaata caagtacaaa acgcaagttg tctttgctta aaactcatnn
     4021 nnnnnnnnac aacctataca gcgcttcgcc gtttcccctg cgcttatcaa taagccccag
     4081 ataccgggca gcatccgtat aataatttgt ttgtcttgca tcaaatgcat attgttccgt
     4141 tacatcattt ctgcttaaat cttggtcgtt gagcaactcg catatattga taacacggtc
     4201 gaatttatcg gcctgcggaa atggtaactg cggctctact ttgcaaattg aggagtgtag
     4261 aacctgttga atatcgtctg ttgtaattgt agtgtcctct acggtatagt tacgctgctg
     4321 gacaaggaca atagaactat aattgcctgg atctgcaaac ttatattcgt aaatatggta
     4381 aattccgtta gaatacacca aaaaaagcgg cctcacgggt ttagttacac ggttcttcca
     4441 cactctgtat ggataataca actggcgcac taaaaaatcg tcagataagt cgcgctttgc
     4501 ttcaaacaag gcgagactgc gaataccctc a
//
LOCUS       DAWBSB010000177         4498 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_3334_length_4498_cov_3.644835, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000177 DAWBSB010000000
VERSION     DAWBSB010000177.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 4498)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4498)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4498
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3334_length_4498_cov_3.644835"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          924 a         1363 c         1372 g          839 t
ORIGIN      
        1 gcatggtgaa caccaaaacg acggccgtgc gcgttatccc ggccatcggc tgcaaggagg
       61 gcgacgtgct ggacttcggc ggccttttgg gctatgcgcc catcctgccc gtgagcacgt
      121 tctcgccgtc ggtgttcgtc aaccgcggcg gacgcatccc cgctccgctg caggcgctga
      181 aaaactgaac tttctctgtg ccgcccctct gcggggcggc tttttgtcgc tgggaagtgc
      241 attccgcgct ttgggcaaag gcttgcgcca gacgctggaa tgatattctt tccataaaac
      301 catcaatcgg aatttgaatt tgaaatgatt tatattcggt tttgccgcag ctataaaatc
      361 ttatacgtct gcgccagcca aaggccgatg tacaataaat actgtgaggt gcgctatggc
      421 agatataaaa atatcagata tgatgcgtat gcagatgaat ttgtgggagc agcacaagga
      481 tacctggtct cccatggaag caaaattcgg caggaatttt atactgtgga tgatggaaga
      541 aatgggcgaa tgtattgcta ttatcaagaa aaaaggcgat tcggcgatta tgcgggaagc
      601 ctctgtccgt gaggcgtttg tcgaggaaat gagcgacgtt ctgatgtatt attttgatac
      661 attgcttcga tatggtgtaa ctcctgaaga aatctcaaat gcatatgtgg ccaaacacaa
      721 taaaaatatg ggcagagatt atcaaaggga acacaataac aaatacgcaa aatgagcatc
      781 atccgctgca cgcaggcttc ccggctatgg gcggcccaaa agagcatgtg ccccgctttc
      841 ctttcggccg aacagtcccc ggaacgcaca aatcacgcaa aaggacgcac cgcacagggt
      901 ccggcgcgca tatttcccct ttttgccgct caaactaagc gcagaaagga tgtgttccca
      961 tgaatatctt ctataaaatc tgcgttattc tcatcatcat cggcggcatc aactggggcc
     1021 tcatcggcct gttcagcttc gacgccgtgg cttggctgct gggcggcagc gcaagtttgc
     1081 tctcccgcgc ggtatatacc gtggttggta tcgccgcgct gtgcgccatt cccagcctgt
     1141 tcggcagcga gccgcaggcg cgccgggcgg aataacgccc cgcgcgtttt ccctctcttc
     1201 cggccgtccg gtgcgccgcg ttcacaaaac gcgttttcgg gcggcccctc tccacggcag
     1261 cgggcctccg ctgccgtttt ttgtcgttcc aatttctccg aaagcccctc ccgggcggga
     1321 tttccagtcc atatcgtgcg ccgcgcactt tacagaaacc ccacttttgg tatataatat
     1381 gctataaatg cgcgcacctg cgcgcacttt gatacaaaca gcatgcagga ggcgcgccat
     1441 ggcggtatac gaaacaggca gcagagccga aacggcagcg ctgggcgagc gcctggccaa
     1501 ggtgctgcgc agcggctcct tggtgctgtt taccggaggg ctcggcgcgg ggaaaacagc
     1561 cttctgtgag ggcatcgccc gcgggctggg ctgcaccgac gaggtctcca gccccacgtt
     1621 cgccatcgtg aacgtatacc gcggccctca gcccttcgcc catttcgaca tgtaccgtat
     1681 ctccaccatg gaggaccttc aggcaacggg cttttttgat tatctggacg acggcgcggt
     1741 ggttgccgca gagtggagcg aaaacgtcgc gccgctcgtg gcgggcgaac ccgctgttca
     1801 tgtggacatc gcagtgctgg acgagcaccg ccgccgcatc accatcgagg gggctcagct
     1861 ttgacgatct ttggcctgga ttctgcggga aagaccgccg gcattgccat actgcgggac
     1921 agccggctga tttatgaatg ttatctggct acgggccata cacacagcga aacgctgctt
     1981 tgcctgtgca aaaacgcctt tgacgccgca gggctttctc ccgcggaaat agacgtgttc
     2041 gccgccgctg ccgggccggg cagcttcacg ggccttcgca tcgggctggc tgccgtcaag
     2101 ggacttgctt tcccacacga cacgccctgc gccgctgtct ccacactgga ggcgttggca
     2161 tacagcgcgg ctgtggaagg cacgctgctg tgcgcgctgg atgcgcgccg gggcgaggtg
     2221 tactgggccg gttttttcgc cgcggacggt gcggtggagc gcttatgccc ggacgagagc
     2281 gcgcccgccg cttccctgcg cgggtttgtg gaaagcgcgc gggagccggt gtggctgctg
     2341 ggcgacggcg cgcagctggt ggacgaggcg ctgaacggca cgggcaagac ccgcctgtat
     2401 cccgaagcct accgcctggg ccgcgccggc ggcgtgtgcc gggctgcgct ggcagcgggt
     2461 gaaacggtgc ccgctgcaca gcttatcccc gattaccacc gcctttcgca ggcggagcgg
     2521 gaacgggcgg cgcgtctgca ggccgcgccc tgaccgcgca aaagataaaa aacataccgc
     2581 gccggcttgc gcccgcggac aaggagttgt atcatatgga gaaacccgtt gcactgggcg
     2641 ccgaccacgg cggttttgcc ctgaaggagg ccgtccgggc gcacctggac gaacgcggcg
     2701 tgccctacgt tgattacggc acgcacagcg aagcatccgt tgattatccc gatatggccg
     2761 tgcctccctg cgaggccgtt gtgcgcggcg aatgcgcctg tgcattgctg ttctgcggca
     2821 cgggcgtggg catcagcatg gcggccaaca agctggacgg aatccgcgcc tgctgctgca
     2881 gcgacgcttt cagcgccaaa tacacacgcc tgcacaacga cgccaacgcc ctttgtctgg
     2941 gcgggcgcgt tgtcggcccg ggccttgcct gcgaactggt ggacctgttt ctggacaccc
     3001 ccttcgaagg cggccgccat gcgggccgcg tcgccaagat atccgcgctg gagcagcgct
     3061 gacatacacg ccgagccatc ccggaaagcc gaacataaag gagactgaaa acaatgagca
     3121 aaacaatgat catggaccat ccccttgtac agcataaggt aagcctgctg cgcaacaaaa
     3181 acaccggcac caaagaattc aaggagcttg tgtgcgagct tgccacactg ctgtgctatg
     3241 aggccacgcg ggatctgccc accgaagagg tggagatcga gacgcccatc gctgtggcaa
     3301 agacgcgcat gctgtccggc cgcaaactgg cgctggtacc gatcctgcgc gcggggctgg
     3361 gcatggtgga cggcatgctg gcgctcatcc cggccgctaa agtcgggcat atcggcatgt
     3421 accgcaacga agagacgctg gagcctgtgg aatactactg caagctgccc gatgatatcg
     3481 ccgagcgcga agtcatcgtt ctggacccga tgctcgccac cggcggcagc gccgtagacg
     3541 ccatcacgca aatcaaaaag cgcggtgcca agagcatcaa gttcattggc atcatcgctg
     3601 cgcccgaagg catgcagcgc ctgcaggagg cgcattccga cgtggacatc tatgtggccg
     3661 cgatggatga aaagctgaac gagaacggct acatcgtgcc gggtctcggc gatgcgggcg
     3721 accgcatttt cggcacaaaa taaaacgttc cctcccgcga tgcggggatg aacgctctat
     3781 gagtggagct atgatatggc aagacgagaa ctgagacgaa aaatcaccgc gacggtgctc
     3841 acggtaactg cgctgtgctg ccttttgggc gtgtgcgcgt ggctgctgct gggcagcaat
     3901 gtgttcagcg acaccatccg catcatgatg ctggtatgca ccgtggtgct gggtgtcgcc
     3961 accattggct tgctggtggc caaggtacag gaaatccgtg cgcttaggga gatcatccgc
     4021 agcgaagacg cccagcagca gagcgaggcc gaaacgcctc gcccggagct ggcccccctg
     4081 cagacactgg accataccac catcgagctt ccgccgctgc cggacatccc ggcgccggct
     4141 gcacagcccg cgcccaagca ggccaagcag cagccgaaaa ccaagcaggg ccgcagcaaa
     4201 aaacgcggcc gtgcaatgga gcagcctgct gctgagacta tggccccggc gcagcccgtg
     4261 ctgcagccca ttgaggtggt gccccccacg cccgtcgccc cgccgcgccc tgtacagcaa
     4321 gcgccgcagc ccgaacagtc gtccgttgaa cgcaagtgga agcccattga tttcaacgcc
     4381 gccatgcagc agcgcctggc cgaagaagcc gccgcacgcc agcgcgccat agaggccgcg
     4441 cggcgcgccg aagaagcacg tttggcggaa gaggcccgcc gcgccgaaga agcacgtt
//
LOCUS       DAWBSB010000178         4483 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_3350_length_4483_cov_2.964770, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000178 DAWBSB010000000
VERSION     DAWBSB010000178.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 4483)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4483)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4483
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3350_length_4483_cov_2.964770"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2424..2433
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3994..4003
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          862 a         1451 c         1145 g         1005 t
ORIGIN      
        1 gagcatctgc cttttaagca gggtgtccgg ggttcgaatc cccgatggct caccacatcg
       61 gaatggactg cgcgccattc aaaaagcccc gcttcggcgg ggcttttctc atgcttccgc
      121 ctcctttccc catccgccca caaaattttg cggcgcaagc tttcgcggag tccaatggca
      181 cctgtgtttc agcgtctgca tggtatcttt tttcatctga aatgaactgc gcgcccgttg
      241 ggagcccggc tttatgacgg gcctttttat tttctcccgc cgtctctaac ttttttaaag
      301 ttttggttga cattccctct tttatagcgt atacttaact aatcgccgaa gtgcgaacta
      361 aattaaagag gtaccacatg ttccaagaca aaacgatcgt ctgcaaagat tgcggacagg
      421 aattcacctt caccgccaac gagcaggagt tttttgctga aaagggcttc accaatgagc
      481 cgcagcgctg caaaccctgc cgcgatgccc gcaaaggtgc ttccaacaac cgccagggcg
      541 gcgagcgcca gatgtttgac gctgtatgcg cttcctgcgg ccgttcctgc aaagttccgt
      601 tccagccccg tacagaccgt ccggtctact gcagcgattg cttccgtaaa taattcgcag
      661 ccgacacgcc tttcggggcg tgtttttgtt ttctgctccg gcaggcttcg tccggcaaaa
      721 acggcgtccc gcgtttcggc gggacgccgt tttactttct tcgtgtattc accagcacca
      781 atcgcaggac gtatagcctt cgtcctcggc ttcctgccgc gtcatttcct tcggtttgat
      841 gaccagcggg cagtccggtg agctgtggta ccgggaaccg aggcgcttgg acacccacac
      901 acgtgtgttc ttctccgctt ccgccgcgct ctgcgccgcg tccgacgtac cggaatcata
      961 tccggcatcg tatccctcgc tggagccgtc ctcatgccct gcggcatagc cctcctcgta
     1021 gccctcggtt ttgcccttgg caaagccggc ggtatagccg tcgtcatagg ttcctttttc
     1081 cgcgatggct gcatcatagc cttcctgata ggtgccgcgc tccgccatag ccgcggcaaa
     1141 acccgcgtca taggttccct tttccgcaat ggctccgttg tagccccagc ggtaaatgcc
     1201 cgccgccgca acgatgagca gcacgacccc gagcgtgagc ccaaaggcca gcacaaagcg
     1261 ccgggtgttt ttcgtcatcg caggcagccg gagccgcttc cagctttcca gcaatttcca
     1321 tataaactcg cggccgctgc ctccacccgg caactctttg tcgtccgtcg gctgcgcccc
     1381 ggcctcctcc gcagcctcca cggccgcaga gatcgccacc gccgttccgc tgcgtccatc
     1441 tgccacgccg ctgaaaaagt catccacgta agcgctttcc atatattgtt gcgccgtcat
     1501 gccgtagcag gatgcgatgc cctgcaccag ccgcagcacg cggcattctt cagcgggcac
     1561 gtccaacgtc ccacagttct cccgccgctg cgggcctgcg ggcatgacct tccagcgcac
     1621 ctgctccagc cgctcttcca gctctgtcat agcgtcatcg aaagagactc cgcgctgttc
     1681 ggcaaactgc cgtacccgtg cagcatacgc acacgctgta ccgcagcgcc cgtccatctg
     1741 gggatattgc tccgccatct ctctgcacct cattccatct ccggccggca aaaaaaatgc
     1801 cgtcccttat tccatgtaaa catatcgccg ttcccacggc agaagccggt caactctatc
     1861 aaattttcac atcatgtttc gcatatcatc cgcattcagc gggcgggcat attataccat
     1921 gacgccgcaa agcggcctca acaaaaccgc acctgctgcg gttttgcatg gttcaatgtt
     1981 ctcaaagccc tgtgcgccag cacaggagcg tgtgcggcgc acacgcgcag ccgcgcaagc
     2041 ggcagctttg ggggttatta taccatatga cgggtccgct gaaaagcacc aatctaagcc
     2101 aaaagatttg ttccgcccgg gcctttcctt tttccaggct ccaccggtgg cgcttttttc
     2161 attttccgct ttatttccga aaaaaacgcc acagcttttc caaagagctg cggcgttttt
     2221 ctcccaatta aacaaaattt aatgtgctgc ctcacatagg atcagatagc ggtgtatgac
     2281 ggaaagcccc agctttccgc ttttctccgg gtggaactgg cagccgatga cactgccctt
     2341 ctgcgccgcc gcacacagcc tgcggccgcc gtataccgtg tctgccaggc ggtgcgcgtc
     2401 ataggcgggc ttcgcctcat aacnnnnnnn nnnactgtga acgaaataca gctccccgcc
     2461 cgggagaaca tcctcgaaca ccgttcctct gaagcttccg ccccgcttct gcggcggcag
     2521 aacgggcgcc catcccacat ggggcacgcg ctgcggcgcg ccgtccgtgt ccacgccggg
     2581 aatgcgctcc actcgcccgg ggatcagtcc cagccccctg tgacgtccga actcatcact
     2641 ttcatcgaag agcatctgca tgcccaggca gatgcccaga agcggcgtgc ctacagcgca
     2701 tttatcggcg atggcatccg ccagagaaag accggagagg cccgccatgc cgtccttgaa
     2761 cgcgcccacg ccgggaagca ccagcgcctt cgcggacagg atgcggctgc ggctgccggt
     2821 aacctccacc tccgcaccga aatactcaaa ggcacgccgc acgctgagca gattcgaaag
     2881 cccgtaatcg atcactgtta tcttcatttt cagcgcacct ctatcccgtg ttcccgaata
     2941 ccggccttga gctgcggcac cgttgttttc ttgtaatgga ggatactggc tacggccacg
     3001 gcggacgcgc ccgcctgtgc cgcggctgcg gcgtcccccg ggccgccgca cccacccgaa
     3061 acgatgacgg gcacgctgac ggccgcgcac aattcacgga tgagcgggca gtccatgccc
     3121 cgttcaaatc cctctttgtc cacgctggtg agcaggatct cgcccgcgcc cagcgccacg
     3181 ccgcgccgtg cccactcgat gacatccttg cccgagtgtt cccgcccgtt gtcataataa
     3241 gcctcataac ccgggccgtc ccggcgtttt ttcgcctcga tgcccaacac catgcactgg
     3301 ctgccgaacg cctcggccac ctgggtaatg atgctctcgt ccttgatggc cgcggtattg
     3361 atggccgcct tgtccgcccc catctgaaga atgctccgca catcgtccac actgcgcagg
     3421 ccgccgccta ccgtgatggg aatgaacacg tcgtccgtgg tgcgccgcac gatagccgac
     3481 aggttgttgc ggttgtacag actggcgaca atgtccatat acagcaatac gtcgacaccc
     3541 tgccggtaat aatctgccgc gaaatcatgc gggtctccca gcttgcgcaa tccttccagc
     3601 tgaatcccct ttaccagatg gtcgttcttt acgtccagac gggcgatgag gcgtattttt
     3661 ttcgccatgc cgcttcctcc tttaacgttt ttcggcaggc acgccgtaat tgcattcgga
     3721 atccccctca aagggcttat ggcgtaactc ccaccggcca ttccggtaca tccagatgtg
     3781 cggtgaacgg aagcggtccg ccaggtgcat gaagtattct ctgtccatgg cgggctgttc
     3841 aaacagcctg ctcgcgtagg ggaaatgcct ttcatcaaga gagaggtaat cccatatctc
     3901 ctgctcaaag cgcgtgggga attcgccgtc atagcgcttg caaagcaccc ttccctctcc
     3961 gcgggtgatt tcttcgtttc ggatctcctg ggcnnnnnnn nnnccgatgc catatttgat
     4021 ataggtggtg taatagaaga aatcgtcgat cttatcgtcg atggaattgt atttggaata
     4081 ggtaccctgc gtgcgctccg ggctgggttt aaagccgccg tgctccacac tgtaataata
     4141 ggcgccctgc gggtgccatt tgagataata gcccagataa tgtacctgaa tgtggttttt
     4201 ttcgatgtcc gaggtatcgc tgggcagata gatggcaagg tccgccgggt ccaccttgaa
     4261 atcctcctcc agctgccgga gagacacgcc gcccagatag atattatccc gggaattcgt
     4321 ggcaaagaaa tgctcgtccc gcagcgcgga ttcattgtcc gcaatgggat tgccgaactc
     4381 ggcttcattt tcgccgtaga acaccagtgg gatgccgaat ttcgcagcca ttttcggagc
     4441 aagctgtttc tggcccagaa tgaacgcctg gaacggatgg aac
//
LOCUS       DAWBSB010000179         4480 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_3353_length_4480_cov_2.348927, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000179 DAWBSB010000000
VERSION     DAWBSB010000179.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 4480)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4480)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4480
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3353_length_4480_cov_2.348927"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2337..2346
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          906 a         1348 c         1254 g          962 t
ORIGIN      
        1 ttgtggagcc ggagggaaaa atctgctggc aggtgcttca aaaagggatg agcgagcgct
       61 gtcccttctg ccccgtgccg cgtctgctgc gggacggccc ttccgcagca ccgctccgct
      121 gggaggaaca taataccagc aacggctgca tctatgaaaa cttcgacagc ctgattcaat
      181 ggcacgatgg ccggctcgtc cactttcagc attcgctgga tgtaacgaaa taccataagc
      241 tctccattgc ggcaaccacc gactaactga cgggcgcgct gaaccgacgc gcgggaaagg
      301 ccgcgctggc ggaaatggcc gctgcatgcg cccgaacagg cgaacctttc tcgctttgca
      361 tgtttgatgt gaacgacctg aaaaagacca atgaccattt cgggcatgct gccggggacc
      421 agctgctcgt tttcattgca gaggcagtgc gcggcgtgct gcaggaaggc gatgtgctct
      481 gccgcctgag cggagatgaa tttctggcgc tgatgcgcgg cgcaccacgg gagcaggcgg
      541 tacagcgtat gaatacggcg ctggcagccc tgcatggagt ccaggcgctg tccgagcagg
      601 cggacgcgtt ttccttcggc gttttaacgc cggagccgaa cggtcccgcg cgctccgtcg
      661 cacagctatt aagcgacgtg gatgaaaagc tctacgaaaa aaagtggcgt ctccacatcg
      721 ccaacgccca gtacacgttg ctgcattccc cacagcgcag ccccggcgtg gacttcacct
      781 atgatacgat ttatctgctg gacgctctgg tgcagagtac cgacgactat gtctacgtct
      841 gcaacatgaa aaccggcgtt ttttgttatc cccaggccat ggtggacgag tttgacctgc
      901 ccggccgcgt cattgccaac gcggcggccg tctggggtgc gaaggtgcat cccaacgaca
      961 agcaggcgtt tctggaagcc aaccaggaaa tcacggacgg ccgtgccgac agccattgtg
     1021 tcgaataccg cgcctgtaac cgccacggcg aatgggtttg gatgcgctgc cgcgggcacc
     1081 tggagcgcga cgcgggcggc gaacctgtat tgtttgcggg tatcatcacc aacttgggaa
     1141 aaaagaacaa agtggaccac ctgacgggcg tatttaacaa attcgaattt gagcacgagg
     1201 tgcgccgcct gctggacgcc cagccccgcg aaggtgtgtg cgtgctgttg ttcggcatcg
     1261 acggccttaa acgcatcaac ggcctctaca atcggttgtt tggggatgag gtcatccgta
     1321 ttgtctgcca aaaactgcag acgctcgctc ctccgggggg cacagttttt cggctggacg
     1381 gcgatgaatt cggggtcgtc ctgcgcggcg gcacattgcg cgctgtccaa ggctatttcg
     1441 gtgctgtgca gcaggccttc agcacacagc aaacctttga cggcagtaaa tttttctgta
     1501 cgctgtcttg cggatgtgcc tttgccccgc acgacggcac cacctatctg ggcctgctga
     1561 agtgcgccag ctctgcgctg gaccacgcca agcgccacgg gaagaaccgc atggaggtat
     1621 tttcgtccgc cattctcggc ccggctgagc gagccatgga gctgacggag cttctgcggg
     1681 aaagcatcga aaacggctgc aatggttttt ctctgcatta ccagcctgtc ttttcgccgg
     1741 acgggcgcct ttgcggcgca gaggcactaa gccgctggca gtgtgcgcgg ttcggcagtg
     1801 tgccgcccgg tgagttcatc gcactgctgg aaaagaacgg catgatcctt cccttcggcc
     1861 gctgggcctt tcggcaggcg gtgcgccagt gcgcggtttt tctggatgag tatccggcgt
     1921 tcagcatggc tgtcaatctc tcctgcctgc agctggagga cccgacccta gtggaatatc
     1981 tggcccaaac gctgcgcgaa gagggcgtga gcgcggagca tatcatcgtg gaactgacgg
     2041 aaagctatct cgcgccgaat ctggaacggc tttcgggact tctggcacag atgcgcggcc
     2101 tggggctgcg tgtggccatg gacgatttcg gaacgggtta ttcctccctc agcgtcctca
     2161 agcacgcccc tgtcgacatc gtgaaaattg accggagctt tgtacagggg ctgtgcggca
     2221 gtgctttcga ccactctttc gtacacctga tggtggagct ttgccacagt gtaggcatcc
     2281 gtgtgtgtgt ggagggcgta gaaactacgg ccgagcttgc cgctttgcag cctttcnnnn
     2341 nnnnnnaagc cgctttccct cgcgagggaa agcggctttt gatttacctt acggagctgc
     2401 attatttttc cggtatccgc gcggcatatt ccttaatttt ttcaaagctc tccgcactga
     2461 tgacatgctc cacacggcag gcgtcctcgg ccgctgtctc gggcgagaca cccaaacgaa
     2521 tcaggcagtc cgtcaacagg cggtgacgct catacacgct ctccgccgcc gcgcggcccg
     2581 tctctgtcag acgaatgaag ccgtcccggt ccaccgtgat gtattccgca ccgcgcaaaa
     2641 ggcccatagc ccggctcaca ctgggctttg taaaccccag ctttgcggcg atgtcaatgc
     2701 tgcgtaccgc ccctttttca ttctggagca ccaggatcgc ctccagataa tcttcacgcg
     2761 attcaaacga acgcatttct cgtccctcct gtctgtgctg ttttggggca tgcggcccgt
     2821 tgaaccaggc ccttgtaata aaaacaggct gtaaactatg ttttagttta ccacatccgc
     2881 gccgctttct caagcgcggc agaatatggc gtcgcccgcg cggcaaaata ccgcgcgggc
     2941 gatgttccgt ctgtttgaat ttcccgtcaa agattaatga gtcatccaca gtgtgaaagc
     3001 tgccgccaat gcagcgcagc caaccagcaa atacagccat tgaaccggac ggcgcgcatg
     3061 ggttttctgc cctggcacag cgaacgcccc tttctcttca ccgcatcggg tgcggcttca
     3121 ttccatggtt ctattaaacc acagaaaaag aacgatcctg ctgccgaaag ccttacaaaa
     3181 aacttacggt ttttatccgg cgcgtcacag cacacattcc gggttgcccc gctgccggct
     3241 cacctcggca agcatggcca gtgtcaccct gtccaacaca tcgttcaccg cgtcatccat
     3301 ctgttcccac agccagcgcg tggcgcactg ctccgcccgc gggcattccg gcgcgtcctg
     3361 tgcaaggcac gctaccgtag caaggtcgcc ctccgtcagc cgcaggatct gccccgctgt
     3421 gtatttctcg ggggcatagg ccaatcggta accgccttgt gcgccgcgca cgctgcgcac
     3481 aaagcccgct tttacaagct gtgagatgat ctgctccata tacttttcgc tgatctgctg
     3541 acgcgcagca atgcttttga ccgaacagta ctcctcaggg tccgccagtg ccagatccag
     3601 catcaggcgc agcgcatagc gcccccgggt agaaattttc atggtacacg catccctctt
     3661 gttccccgca aaaggtgcag agatttcttc tttttattat aaacggggaa ttgcgcggcc
     3721 gcaagtcaaa accggcaaaa tagatacgct ttttactgct tttctccaca aaaagacaaa
     3781 gcaggccgcc ccgcgggctc tcttttccag aaaagccctg cggaacggcc tgaaaacggc
     3841 gcagagcgta ccggcgcttt acagatgatg cgcaaagccc tcggccagct tctgcagctc
     3901 gcccttcgtc gtcacacgcg cgtcccattg caggatctgc gcgcgcaccg ttccgggcag
     3961 cgcgatccac acatcccgca attccgggtc cgcgcggtaa tattcatcca aaagcatgct
     4021 cagccctccg ttctgcgtcc gccgtcgtac agcttggcca gattttcagc aaatacagcc
     4081 aggctgtcca gatcctccag ctccacatcc gcgtggcgta cggcattttg tacgggagcc
     4141 ggcaggtttt gataaaatac gagcatttcc ggcgtgtaat cctcaagatt ctgcattctt
     4201 ttcacctctt gcggttagga tgcgcggcgg ccgggaaaat attcaaacgg taaaaaaccc
     4261 ctgcggcggc cgggaaaacc catttggggg ttggcactgg cgcatggggc gtggtatgat
     4321 aaggtcagga atcaatgtaa atgccactgg cggcgcagtg cgccgctcaa gcgaaaggat
     4381 ccttgttatg caatacacat cactgcccga cgggttcacc attcaggagc tggacgcctc
     4441 ccatctggag caattcaacg cactgctgcg ctatgcgttc
//
LOCUS       DAWBSB010000180         4459 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_3373_length_4459_cov_3.077657, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000180 DAWBSB010000000
VERSION     DAWBSB010000180.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 4459)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4459)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4459
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3373_length_4459_cov_3.077657"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1912..1921
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2320..2329
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          877 a         1240 c         1512 g          810 t
ORIGIN      
        1 tatcgtttta cggagggatt tttccatgac cattaccaaa gacaccattc tcggcgatat
       61 tctggacgcc gcgccggaga ccgcgcccct gttcattgag atcggcatgc attgtctggg
      121 ctgcccgtcc gcccgcagcg agacggtgga gcaggcctgc tatgtccacg gccaggacgt
      181 tgacgcgctg ctggccaagc tgaacgcggc cattgccgga aagtaagctg ctgcagcccc
      241 cggttctgga cgcacgcctt gccgccgcgg ccgcttatgt acggccgggc tgcgtggcgg
      301 cggacgtcgg ctgcgaccac ggcaggctgt ccgtggcgct ggccgcgcag ggcaaatgca
      361 gaaagatcat cgcaagcgat atccgcccgg ccccgcttgc cacggcgcag cgcctcgtta
      421 ccctgcatgg ctgcggggac gtggtggaat gccgccttgg cgcggggctt tctgtgctgc
      481 gcccgggcga agcggacgat atcgtcattg cgggcgtttc gggcgtgacg gtgtgtgaga
      541 ttttggagca ggctccggaa gcgttctatg ctgcgaaacc cgacatgcgc ttcatcttca
      601 tcccggctac aaagcaccct tttttgcgcc gctggctggc acagcacggc tttgcgctgc
      661 tggatgagac gccggttttg gccgccggac ggtattatac cgtgatgcac gcggcttata
      721 ctggcggaac aaagacgccc gatcctctgt ggtgcgtcgt gggccgtgcc gggcgcgggc
      781 cgcatgcggc aggctatctt gcccgggagg ccatgcttct taaaaaggag gcgcggggag
      841 ccgcgccgga ggaatcaaaa cggctgctgg cgcttgccgc cgcggtggag gaggctgcga
      901 atacatgcca agcctgacag agcttgcgtc ttatctggaa agcatcgccc cggcgcagat
      961 tgcggagagc tgggacaacg tgggcgtttt ggtgcgctgc gcgcaggagg tgacgggcgt
     1021 gctgtgcgcg ctggacatca cccccgccac ggtgcgggaa gcgcgcgctg tggggtgcaa
     1081 tgtcattgtt tcgcaccacc cggtcatatt tcgccctttg aaggagcttt gtcaggatga
     1141 cgttcccgcc ctgctggtgc gggagggcgt ttccgccgta tgcatgcata cgaatctgga
     1201 caaggcgcag ggcggtgtaa acgacgtgct ggcgcaggcg ctgggcctgc gggacacaga
     1261 gccgtttgcc ggaggcatcg gacgtgtcgg cgcgctgccg gaaacgatgg aagcggccgc
     1321 gttcgcgcaa tttgtgggca aggcgcttgc cgcgcccgtg aaatgggcgg acgcccacgg
     1381 cccggtgcgc cgcgtggccg ttgtgagcgg cagcggcggc gatttttccg cagaggcggc
     1441 cgccgcgggc gcggactgcc ttgtgacggg cgaggccggg caccatgacg cgctggatgc
     1501 attggcggca ggcgtcacgc tggtggccgc gacgcatttt gccacggaga cagccattgc
     1561 cggcgtgctg gcgcaccgga tcgccaaagc gttcccgggc ctgcgtgtac agcgcagcgc
     1621 tgcggatgcg gaccctttcc tttattttta acaacgggga agcgcggtcg tttggcaggc
     1681 gcatcgctgc ttttgacgcg gccgcgcggg cggagccttg gcctgcctgc atacataaga
     1741 ttttttggaa cgcagagacg gtctttgacc gcttgcgtta tattatataa tgtaataata
     1801 gacagcaaag agtaatatgc caaaaacagg cgcgcgccgg acgaacgacg gttccggccg
     1861 atggaaagag atctgaagaa gatggatgcg gctggaatgc tgatgatttt tnnnnnnnnn
     1921 ntttccccag cgcggtatgc gctggggccg tatgctttgt gaaatatctg cggcgtgagt
     1981 gataacgcgc cggtgaaacc gctgccgggc gtgtgcggga tgaaatgctt tggcccggat
     2041 ggaaaaagga gaaaacaatg gctttagacg ccgctgccct tgcgctgtgc gcaaaagagc
     2101 tgtccgccgc actggcggac gcgaggatcg ataaaatatt cgagccgacc cgggacgagg
     2161 tcgtactcaa tctgcgcacg cgcacggaca atccgcgcct gttcctttcg gcccgcagcg
     2221 gctcggcccg cgcgtgcctg acaaaagaga cgtttgagaa tccgcagacg ccgccctcgt
     2281 tttgcatgct gctgcgcaag catttcacgg gcggacgccn nnnnnnnnnt gccggacgag
     2341 cgcatcctgt tttttgattt ccagtgcacc aacgagatgg gcgacaccgt ggtgaatacg
     2401 atggcggccg agttgatggg ccggtattcc aatctggtgc tggtgcagac gaagaacccg
     2461 gcaaacccgg cggcggaagg gaaaatcatc gacgcgctca aacgcgtgga ctttgaggac
     2521 agcgaggttc ggcagctttt gccgggcctt gcttacacgc tgccgcccca gccgcagaag
     2581 gcgtcgtttc tgggcgtcag cgcggcgggc atcgtggcgg cggtgtcgca gaaagaactg
     2641 cccgtggcgg aggcactcat caagacaacg ggcggcgtgg gcccggtggt ttgccgcgaa
     2701 gccgcgtacc gcgctttcgg cgggcgggac gtgaccgcca gccggatgac ggagcaggaa
     2761 aaggcggctc tgctggaggc ggtgaccttt gttcaggcgg cttacgcgga cggcggcacg
     2821 ccggtggcgg ttgtggtgga cgggcgcccg gtggaattca gctttttgcc gctgacgcag
     2881 tacagcgggg cggatcttgt ggaatacggc agctattcgg aactgctgga gagctactac
     2941 gcagccaagg acaaggcgga gcgcctgcgc cagaagagcc gcgatttggc aaagaccgtg
     3001 cacaatctgc acgagcgtgc cgtgcgcaaa gccgccgccc gccgggagga acaggcgcag
     3061 agcgcggctt cggaggaact gcgcgtttac ggcgagcttc tgagcgccaa tctgtgggcc
     3121 atccagcgcg gcgcgaaaag cgtgacgctc acgaactact acgacggcag agaaatgacc
     3181 atcccgctgg atgtgcgtct ttcccccagc gccaacgccc agaagtattt taaggagtac
     3241 aagaaaaagc agacggccgc ccggatgctg acggagctga tcgcggagag cgacgcggag
     3301 gcggagtatc tggctacggt gcaatacgag gtggagacgg ccgagggcga ggccgcgctg
     3361 gcggagatcc gcgccgagct gaagagccag ggatacctga agtactataa ggcgaaggac
     3421 aaaaagcaga agcctgcgga ttttctgcgg tatgtgtcct cggacggatt ccccatcctg
     3481 gtgggacgga acaatgccca aaacgacagg ctgacgctca agaccgcgcg gggcagggat
     3541 gtctggttcc atgtgaagaa cgcgcccggc agccacgcgg tggtgctcag cggcggacag
     3601 cccgtgccgg acgctaccaa gacggaggcg gccgtccttg cggcggtgca ctccagccag
     3661 aacggcggcg caaaggtgca ggtggattat accgaggtgc ggaacgtctg gaaggcgaac
     3721 ggggcaaagc ccggcatggt gctgtacgat ccttacgaga cggccgtcgt cacgccggac
     3781 ccggcgctgg cggagcgcct gcgggcgaaa tgagcaggcg cggcaaaata accaagcgcc
     3841 gcccggtgaa aatcgggggt tctgtgcaaa gcgtaaaatc gaaaacttct tataaaattt
     3901 atcaaaaacc cttgcattgc gcaggggttt ttggtattat tattaggcga ctgcgaatgc
     3961 cctgcatttt cgcagggcag cgatatgcgt tgacgcggga ggttgcggca ctttgtgccg
     4021 gtttttccgc cgagtatgtc cgatcaatga accgggcgaa agaatactga atgcaaaggg
     4081 atacgtttgc gggaagttgc tcccctcaaa ccaatgtcca cgctaatttg ctgaaagtgc
     4141 ttccggagga actgcatggc ggttcaccgg attttctgat gcaatggcgt gaaacacccg
     4201 gcgcagtgtt cagtatttat cgaatggttc gtcccacgca aaattttaag gaggcgaaac
     4261 aaatggcagt caaggagaaa atcaggattc gtttgaagag ctacgatgcg tccctcatcg
     4321 acagcgcggc ggagaagatc gtggaaacgg cgaagcgcac aggcgcccgc gtgtccggcc
     4381 cggtccctct tcccactgac aaagaggtca tcacgatcct gcgcgcgcta cacaagtaca
     4441 aagacagccg cgagcagtt
//
LOCUS       DAWBSB010000181         4450 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_3380_length_4450_cov_3.566780, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000181 DAWBSB010000000
VERSION     DAWBSB010000181.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 4450)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4450)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4450
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3380_length_4450_cov_3.566780"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          842 a         1377 c         1454 g          777 t
ORIGIN      
        1 tttttcattt aaaataaccc cgcgacgccc cgcagggcta gtgccgaggc gtgtagagcg
       61 aagcgaccgc cgaaggccgc cgtgcgcggc gaacggggcc gcaggccctg ttcgtaggca
      121 gcttggcgca aaagcgaagc tttttcgaca agctgtacag ccccggcttc tcgacagaga
      181 agccggggcc ttgtgcgctg tgcattattc cggcgtttgc ggcgccggac gggtataccg
      241 gacgatcagc acgcacacgc ccacggcggt gacggcctcg gtgacgggca tggcgaacca
      301 gacggcgccg ggcccgagca gggccggcag cgcataaatc agcgcgccgc tgagcaccag
      361 cccgcgcagg accgaaacag aaaacgaggc gcggggcttc atcagggact ggaaatagta
      421 ggtggagaaa atattgagcg gcagcagcag gaacgaaacg ccgtagctgc gcagaatggc
      481 gggtgcgatg cgcaggatgt ccccggtggg ggacatgaaa atgcggatga acccgttggg
      541 aaacagaaag acgagcagcg tccacaccgc gctgaaaaag gcggcggcca tcagcgcgta
      601 cttcagcgcc gcgcggatgc gtccccattt gcccgcgccg aagctggccg aaaggatggg
      661 ctgggcggcc tggcccacgc tgtatgcaca gcactggacc atggtgctca cgttcacgat
      721 cacaccgtac acggccagtg cgtccgtgcc cagtccctgc aggatctggc ggttgaacag
      781 catggtcagg atgcccatgg cgatgtcgag aaagaacgtg gaaaagccgg tggtcagaat
      841 ctgccgcagc ttggttccgg catttcggaa gcggacgggc cgcagcgtgc agcgccggga
      901 gaaaaagtgg gacagcatca ccgtgaaggt gatgcccgag ccgatggctg ttgccagccc
      961 cgcgccgaat acgcccatgt cacaggtgaa cacaaaaaag taatcgccga acacattgaa
     1021 gacgccgccc gccagcaccg ccgccgtggc aagccccggc gcgccgtcgt tgcgcaggaa
     1081 cgccgccagc atctggttga acaggaacag cggcaccacg aatttgacgg ggagcagata
     1141 ttgctgcgcc agcggcagca gcgcgtcccg cgcgccgaac aggcgcagca gcggcgcgtc
     1201 gaaaaatgcg atggcgagcc agctcacggc ggcaaagaac acggtgcccg ccagcgcggc
     1261 ggtaaaatac tcgtcgcctt ctttctgcct gcccgcgccc cgcgccgcgc ttagcagaac
     1321 cgagccgccg atgcctgtca gcagccccag gctgtaaatg atgttccaca ccggcgcgac
     1381 gatggccagc gcggccgtgc cctccggccc ctggtactgc cccaccatgg ccgaatccac
     1441 caggccgtag atggagctga tgagcgcgct gccgaacgcc gcgcccagat acctgaaata
     1501 aatttgcctg acgtttcctt tcagcagagc cattgagatc cctcccgttg ttttgcacac
     1561 cccgcgggca tcaaaaaagc cggataaaga aacaggcgtc tcagcctgtc accttatccg
     1621 gcccatcatt tccaagtgaa tgatctgccc tccgagtgag cagttgacat tatactaaaa
     1681 caaaagagcg aagtcaagcc ctgtcttacg gcgcggggca cggcgctatg gcagggcggg
     1741 ctgctccggc ggcaggttgt cctcggtgta gtatcccaca gcgccgcccg aggggacaag
     1801 gcatccggtg gcggggtcgt agcggcgtgc aacgacgccc tccggcacgg gaaattccag
     1861 atagggcagc ccttcctgtg cctgctgcat ataatcccgc cagagcgtct gcagcggttt
     1921 gcccgtgcgc acgccctgct gggacaggtc ggccgggacg tcgtagcccc accagcccgc
     1981 cgtgacgtaa taaggcgtca ggcccacaaa ggtgaagtcg cggtaatccg tcgtagtgcc
     2041 cgttttggcg gcggcttcca tctccccggc gggcgccatg ccccgggcgg tgccgcccgg
     2101 cgcggtgagc acgccccgca gcagccggtt catgatataa gccgtctgcg ggctgacggc
     2161 ctgcacggca tagctgtcct gcgcggggtc cagataagtg gagccgtccg ccagcgtgac
     2221 tttggtgtaa tagagcgcgg gacggtacac gccgtcgcgg aaaacggaat atgcgttggc
     2281 aagctgcacg ggcgtaacgc cgtgggtctg gctgcccagc accaaagggc cgagatcgtt
     2341 gtccctctcc gggtccagat gggtgaggcc caggctgccg tacacaaagt cgaacagcct
     2401 gcgcttgccc agcatgtcgc cgatctgcac cgggacggta ttcagcgact gcgcaagcgc
     2461 atccgcaacc actacgggcc gccctgtgcc gggcccgttg taattggtgg gccaggcgcg
     2521 ccaaacgtcg tcccgcgcct ggttggcggg gtcgcccggg tccagcgaaa ggcccagctt
     2581 gcggcagtat tccgtgttca gcatgcggtg gtcctgctgg gtgtagagcg gcgcgtccgc
     2641 aatgagggag gaatagttga tgacgccgtt ttcaatgccc agcgcatacg cggcgagggg
     2701 cttcattgtg gagccgatct gacggggcac gtcggcggcg cggttcagcg aaaggtcgta
     2761 ccgtttttcg cccacgccgc ccacaagcgc cagcacgtgg ccttcatagt ccatcgtggc
     2821 cacggcggcc tgcgtgcgga tgttcctgta aaataccagt gtgtggacgc cgtcggcatt
     2881 gggctgcgcg ctggagccgc ggagcagcgt tgtgccgtcc tgctcataga cgggcgtgtc
     2941 gtccggcctg aacaccggct cgccctgtgc gttcagcggc aggccgtcgt ctccgtagga
     3001 aacggcgtcg gcgctgtcga agggaatgcc tgtgtcggct tcttcctgcc gccacagagc
     3061 ggggaagtag ccgtcctcgc cgtcgttttc catgaaagct tccagcatgc gctgcaggcg
     3121 caggtccacg gtggaaaaga tgcgcaggcc cccggttcgg atgcgggtga cggcggcggc
     3181 acggccgcag ttctcttttt ccatgatatc gtccgccagc cgttcaaaca cagcgtcggt
     3241 gaaataagag ttcacctttc ccctggggga ggcggcagcg gcctcctcgc ggctgcgcac
     3301 gcccagcgga gcggccgccg ccgcgtcgta atcggcctgg gagatgcgct cctggtccag
     3361 catcagtttg agcacccagt tgcgccgttc cagcagcttt tccgggttgg agtaagggct
     3421 gtacgccacc gggctgcggg ggatggaggc cagcgtggcg gattcggcca gcgtgagctg
     3481 ggaggcgtct ttgccgaaat actgctgtgc gccggcctgc atgccggaga gcgtgcccgt
     3541 gagcggcgcg atatttaagt aggcctccag gatcatatcc ttggagtagc gcccggccat
     3601 tacgatggcg cgtgaaatct ctttgaattt gccggaaatg ctctgggcgc tgtcgccggt
     3661 gaggttttcg ataagctgct gttccagtgt ggacgcgccc tggcggctgc cgtacaggcg
     3721 gtggcccgtc agctcgttga cggccgcgcc gaggatgcgc cccgggttga tgccgaagcg
     3781 ctggttccag aaattgcggt cctccacact gacgacagcg tcccgcagcg tttgggggaa
     3841 ctgctccagc ggcgtccaga cgcggtcgtt ttcgccggta aaaacagcgt attccgtcca
     3901 atcgccgccg gccgggtccg ctgcatatac gatggagttt tccgccatct tcagcacata
     3961 gaggtcgggc atgtcggcgt ccgtgccgga caggcgggcc agatacgagg caaataccac
     4021 ggcggccacg ctcgccagca tcacgcccac gcagcagcag cacgccgcga tatacccgat
     4081 gcggcgcaga acgcgccgtg cagttttgcc gccgcgcgtt ttgaggggcg ctgcggccgg
     4141 agcgccgccg tcctgcctgc cggggccgga aacggccctg cgttcgggaa gcttcacttt
     4201 cattccactg ccttctttcc ttggatcgtt aaatgctccg gcacgcctgc ggcgcgcggg
     4261 aaacagctct ttcccgtttg aaacacaata cgttttcaaa agacggattc ttgcattttg
     4321 ccgcgattgt aacgaattgg taatgaatgt taaggcaata ccgcacaatt ctaagagtcg
     4381 aatcgcgccg gccggttttt tggcaggtta aacaaaaagc gcagcgaata ctttgtgtat
     4441 tcgcgagcat
//
LOCUS       DAWBSB010000182         4446 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_3382_length_4446_cov_3.075837, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000182 DAWBSB010000000
VERSION     DAWBSB010000182.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 4446)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4446)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4446
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3382_length_4446_cov_3.075837"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1022 a         1299 c         1379 g          746 t
ORIGIN      
        1 gccatgtgga ccggatggtg acgctggggt gcatgcccac tacagagacg gtgcccatcg
       61 acaagggcat tgacatctgg cacaacttcg gcacgcagta ttttctggag cgccgggaga
      121 tcggcatctt caacatcggc ggcgcgggca gcatcacggc ggacggagta aagtacgagc
      181 tgggctacaa ggactgcctg tacatcacgc aggggacgaa ggaagtgctg ttcgccagcg
      241 aagacgcggc gaagcccgcg aagttctaca tggtgtcggc cccggcgcac cgtgcgtatg
      301 agacgaagct catcaaaatc gaggacacgg cgaaaaagcc gctgggcgcg atggaaacga
      361 gcaacaagcg agtgatcaac cagttcatcc acccggacgt gctgccgacg tgccagctat
      421 ccatgggcat gacggtgctg gagccgggca gcgtatggaa cacgatgccg gcgcacaccc
      481 acgagcgtcg gatggaagtg tatatgtatt ttgaagtgcc ggaggacaac gtggtgttcc
      541 acatgatggg cgagggcagg gagacgcggc acatcgtgat gcagaacgag caggcggtga
      601 tcagcccgag ctggagcatc cacgcgggcg cgggcacgag caactacacg ttcatctggg
      661 cgatgggcgg cgaaaaccag gcgttcgacg acatggacgt gatcccgacc acagagcttc
      721 ggtaaaagca cagaaaacag ccgcgggcgg aaagcgcgcg cgggcaggag ggcaaaaaaa
      781 tggaaatgaa ggatttttct ctggaaggca aggtagcgct ggtaacaggc gcgagctacg
      841 gcatcggttt tgccatcgcg gaaggcatgg cgaaggcggg cgcgaccatc gtattcaatg
      901 acatccggca ggagctggtg gacaagggcc ttgcggcata caaggaagcg ggcgtggaag
      961 cccacggata tgtgtgcgac gtaacggacg aggacgcggt gcaggcgatg gtgaagcaga
     1021 tcgcccagga agtgggcgtc atcgacattc tggtgaacaa cgcgggcatc atcaagcgca
     1081 tcccgatgtg cgagatgacg gcggcgcagt tccggcaggt ggtggacgtg gacctgaacg
     1141 cgccgttcat cgtgagcaag gcggtgatcc cggggatgat cgaaaagggg cacggaaaga
     1201 tcatcaacat ctgcagcatg atgagcgagc tgggccgcga gacggtgagc gcgtatgcgg
     1261 ctgcaaaggg cggcctgaag atgctgacga agaacattgc cagcgagtac ggcgaataca
     1321 acatccagtg caacggcatc gggccgggat acatcgagac gccgcagacg gcgccgctgc
     1381 gggagcgcca ggcggacgga agccgccacc cgttcgacca gttcatcatc gcaaagacgc
     1441 cggcggcgcg gtggggcacc acggaggacc ttgtgggccc ggcggtgttc cttgccagcg
     1501 acgcttccaa cttcgtcaac ggccatgtgc tgtatgtgga cggcggcatc ctcgcctaca
     1561 tcggcaagca gccgcagtaa aaccgaatct ttttggaacc gaaaggccgc ccgccccgtg
     1621 tttacggggc gggcggcctt ttctttttgc actcctttac ttccgctcag cgccaaagga
     1681 accagatgag caggtatccc gttgtgacgg caatgagaga gaacggtacg ccgattttca
     1741 taaaatcccg gttggagacc gtgtagccct ccttgcgcag gatgccgatg cccgtgatgt
     1801 tggcgcttgc gccgatgggc gtcaggttgc cgcccagggt cgcgcctgtc agcagcccaa
     1861 aatagagcag atacggctcc acgcccatga gtgcggcaat gccgctcacc acaggcagca
     1921 tcgttgccac ataagggatg ttgtcaataa atgcggagaa aagcaccgaa gcccatacga
     1981 tgagcgaata gatggcaaac aggtcgtctc ccgcgatgtt gacaaacagg cggctgatgg
     2041 cgtcgatgac acccgcccgc gtgatgcccg cgatgatgac gaacaggctc gccagcaaaa
     2101 gcagcgtctg gccgtccagc tcacggaaga actgccccag cggagccagc gaccgacgct
     2161 gacccgcttt gcgcaggaag cccacggccg ccagcagcac acagatgacc ccattggtca
     2221 cggcaggctt ttccggcaca aaggaaacga tgataagcag agtcaccgta gaaacgagca
     2281 gaagcgtggg gacagtgtcc gttacctgtg tgcgctcacc ggattccacc ggctggcgca
     2341 ggtttcggaa gtaatacagc agcacgcacg cggatgccac ggcacccacc tgtaccaccc
     2401 aaaaaatccc gggcctgccg cggaacacga agaaatcaag aaaattcatg ccggcatagc
     2461 cgcccagcag aatggatgtg gtatctccca ccagcgtggc cgcgccctgc agattggagg
     2521 acaccgcaat ggcgatgatg accggcacag ggctgatgtc cagctttttg gccagcgcca
     2581 gcgccacagg cgcgaccatc agcactgtgg ccacattgtc cacgaaagcg gagatcagcc
     2641 ccgcaaacag ggaaagcgcc accaccgccc atttgatgtt tggcactttt tccataatga
     2701 tatccgccat cagcgcgggc atgcgtgatt cgataaacag caccacagtg cccatggtgc
     2761 ccgcaatcat cagcagaacg ttccagtcta tggccgcagg cacctcagcc cagggcagga
     2821 tgcccagcac cacgaaaagc agcgccgacg cagcggcgat atacgcgcgg tatttgccga
     2881 agatcaaaag cagaacatag gtcgccaaaa acagtgcgag cgcaagatac atgcccggtc
     2941 tccccttcca tcatccgtgt cccggccgcg cggcgcgggg cttttgcccg cggccgcgca
     3001 ccggcctgtc cacagtatat agaacgggcc cgcgggccgt caatcgaatc gggacgaact
     3061 tagcggtatt ttagcggccg gcggcgcagc gcatgcttca ccgttcctcg tagatctgct
     3121 ggctgccgtc gatggcctgc tccagatacg cgtcgatcca ccgctccgct ccgtccatgt
     3181 cccgcctgca caggtaattg tacagcgttt ccgtgttgcg gcaaagcgcg ccgatgccgt
     3241 acatgcggca gaaacgcagc cacagggtga taacgggcgc cttgaacgaa tagtagatga
     3301 gcggcagaat gctgttgccg cccgcaagcg caagctcgtg ctggaaggca aaggccgcct
     3361 cagccgcctg cgcgttgttc tgcgctgcgg cgatgcgcgc cacaatatcc cccaggcgtg
     3421 ccagcgcttc atccgaggca taacggatgg cacgctgcgc agccagatgc tccagcgcgc
     3481 gccgcacctc cagaatagag cgtatctcat ccttgcccag cacgccgccc tgatactcca
     3541 tgatggcgat aagcgtggaa aggttcccct tgcgtcggta atccgccacg aaagttccct
     3601 gccgcggccg cacctccaga aagccctgct gggccagctc cgccagaccg ccgttcacca
     3661 cggcgcggct cacctgcatc tgctgggcca gttcccgctc aggcggaagc tgtgtaccca
     3721 tgggcagctc gtcggacaat atcatcccct gcagctgctg gacaaacagg tccttcaggc
     3781 tgggagcgga cagctttgca aattccattg tgtgcctccc cttctggtat tggttgtacc
     3841 acatatcact ttttgatttt atcatacatt ttgccagagt acaagcccgc tttataccac
     3901 actttcacaa aaagggagac ggccgattga cgggccgaag caggaatgtt aaactattga
     3961 cgaagattgg tatagccacg ctatcaagca aacaagggag agatacctgt ggacgaagtg
     4021 aggattttgg agatcaaaca gagcgtattc gccaacaacg accagcgcgc cgagcttttg
     4081 cgccgggagc tgaaagaaaa gaggatcttt ctgctcaacc tgatgagctc gcccggctcc
     4141 ggcaaaacga ccaccctgac gcgcaccatc gagcgtctgc gtgacgaatt caagatcggt
     4201 gtgatggagg cggacatcga ctccgatgtg gacgcgcgta ccattgcggc gacgggcgtt
     4261 aaatccattc agctgcacac gggcggtatg tgccatctgg acgcggatat gacgcgccag
     4321 ggcctggacg gccttgcagc gggcgacgtg gagcttgctg ttttggaaaa cgtgggcaat
     4381 ctggtatgtc cggcggagtt cgacacaggc gcggtgaaaa acgcggccat cctgtcggtg
     4441 cccgag
//
LOCUS       DAWBSB010000183         4434 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_3388_length_4434_cov_3.638274, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000183 DAWBSB010000000
VERSION     DAWBSB010000183.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4434)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3388_length_4434_cov_3.638274"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          696 a         1475 c         1382 g          881 t
ORIGIN      
        1 cttcacgtcc acgatgttgg ggaactgctc cagcatcagc tcgttgatca cgcggcccag
       61 cgtggtgggc gccagctttt tctggtcccg ctccacataa tcgcggtcga tgatggtggt
      121 gatgatgggc gcataggtgg acggacggcc gatgccgttt tcctccagcg ccttgatgag
      181 cgtcgcctcg gtgtagcgcg cgggcggctg ggtgaacttc tgctcgggct tcagctcccg
      241 cagcttcagc accatgccct cctccagcgg cggcagcgcg gtctctttct tctctttctc
      301 gtcggtggct tcctcgtaca gcgtggtaaa gccgtcgaac gtcaccacat agccgctggc
      361 acggaacagg tagcccgccg cggtgatgtc ggcagagacg gtgtcctgct ggcagtcgct
      421 catctggctt gcgataaagc ggctccagat caggcggtag agcttagccg ggtcgcccga
      481 aacgctcttt tccacctcgg cgggcgtcag agcggggttt gtgggccgca ccgcctcgtg
      541 ggcgtcctgc gcggccgtgg cgcttttgct tttgtaggtg cgcttctggg gacacacata
      601 ttccgggccg taggtctcgg tgatgaactc cttggccgcg gcctgcgcct cgtccgcaat
      661 gcgcaggctg tcggtacgca tgtaggtgat caggcccatg gtgccctgcc ctgcaatatc
      721 cacgccctcg tacagcgtct gggccgctcg catggtgcgc gttgccgtaa agcccagccg
      781 gcggctggcc tcctgctgca tggtggaggt gataaacggg ggcgcgggca cctttttccg
      841 cttgcccttg ctgatggagg acacggtata agccgcgccg tccagggcct tgacgatctc
      901 ggcgctctgc ccggcgctcg tcacggccag cttcctgccg cccgccagcg ccgtgaggcg
      961 cgcggtgaat ttttttgtgg aggacggggg gctgagcagc gcgtcgatgt tccagtattc
     1021 ctcgggcttg aacgcctcaa tctccttttc cctgtccacg atcaggcgca cggccacgct
     1081 ctgcacacgc cccgcggaaa gcccccggcg cacctttttc cacaaaaagg gcgagagctt
     1141 gtagcccacc aggcggtcca gcacacggcg ggcctgctgg gcgttgaaca ggtccatatc
     1201 aatggcgcgc ggcgcggcca tgccctgcgt cacgcccttt ttggtgatct cgccgaaggt
     1261 gacgcggttt tccgccgcag tgtccaggcc cagcagattg gccagatgcc agctgatggc
     1321 ctcgccctca cggtccgggt cggtggcgag gaacacttcg tcgctctcct tggccaggct
     1381 cttaagctcc ttgatgattt tttccttgcc cttgatgttg atatactggg gagcgtagcc
     1441 gtgctcgacg tcaatgccaa gctggctctg gggcaggtct cggatgtggc ccatggaggc
     1501 cgtcacctca tatccgccgc ccaggtactt gcggatggtc ttggctttcg ccggggattc
     1561 tacgatcacg agttttgaca ttgtgttacc gtcctttttc gggatttggc cgagtccggc
     1621 agacagccgg ggcagcttcc gccgcgggag cagccgtcct gcggacatgc ttttgggcgc
     1681 cgccggggcg ctccgaatac ggccgggcgc cctccggcgc cccttgcggg cctcccttgc
     1741 gcggcccgcg gctctttcta ttatgcgata ataagagaaa aaaaacaatc caaatctatg
     1801 cggccgcctc gaggggcatg cccttcaaac agcacagggc cgtcattttc tatttcagct
     1861 ggaactgccg gcccgcaagc tgccgggaaa agcccgcaag ctccagctcc gtcaaagccg
     1921 ccatcgcctt gggcggcggc aggcccgtct ttgcgcacag cgcgtccagc ggcagcggcg
     1981 ccgcggcgct catcgcgtcc cgcatcctct gtgcgtccag gctcagcggc gcgccgtccg
     2041 gctttgcttt ttccggctgc tccggctggg ccagaccata ccacaaaagg atatccgcgc
     2101 ccgatacagc gggcaccgcg ccctcgcgca gaagctggtt cgtgccctcg cacagcggcg
     2161 aaaaaatgct gcccggcacg ctgaaaacgt ccctcccggc ggccagcgcc gcggccacgg
     2221 tgttcatggt gcccgaacgg cggcgcgctt cggccacgca cagcccgcgg gacagccccg
     2281 caatgagccg gttgcgctgc agaaaataac cgcgctgcgg cggggtgccc ggcggatact
     2341 cgctgagcac caggccctgc cgctcgatga cgcctttaag cgtgcggttg gcggcgggat
     2401 agcactgatc atgcccgaag gcgatgcacg ccaccgtggg cgcgcccgcc tggacggccg
     2461 ccttatggct ctcgctgtca agccctgtgg caaggccgga gacaagcgct acgcccgccc
     2521 gggccaattc gcccgcgatg gacgccgtcg cctccacacc gtaggcggag gggcgccgcg
     2581 ttcccacaat ggcgaatgta aagcagcggt tgggaatgcc cgcgtcgccc cggtaataca
     2641 gcacgggcgg cggtgcagac actgcgcgca gcagatccgg ataatcttcg tcggcccacg
     2701 tcagtacgtt cacgccatgc cgggcgcagt cgtccagacg ggcggtataa tcctccggcc
     2761 gcgcggcccg cagcgcggcc agctgcgccg gcgtgaagac catggagagg tcttccttcc
     2821 agcacgcctg tgccagcgca cgcgcgtccg gaaacatgct cagcgccatg ccgctgttcg
     2881 gcgcgccggg gccaagcgcc gctgccatcc aaagccacgc agtcttttcc tccagcgcct
     2941 gtgcgcgtgc accgcccatc gctacacctc cctgaaatgc cacagcagca cgccgcccgc
     3001 gacggcggcg ttcagcgatt ccacgcccgc cgccatgggg atgcgcacgg ctttgtcggc
     3061 cgcggctacg gtcccgggca gaaggccgtt gccttcgttg ccgatgagaa gcgccgcccc
     3121 gggcaggcgc tgcgtgtcca cgcgctccag cgggacggaa ttctgcaggg ccgccgcaaa
     3181 gcttgggata ccgcacgctt tgaacgcggc cagcgcctgt ccggtttcat ctgcaaaaat
     3241 gatgtctacc ttggccagcg tgcccatgga ggcccgcgcc gccttggggg agaatgggtc
     3301 ggcgcaattg ccgcacagca ccgcgcccgc gaagccgaac gccgccgcgc ttcgcagcag
     3361 cgcccccaca ttggcggggt cctgtatgtg ctccaggcac acatagcgcc cgccgccgcg
     3421 caccgcctcc agcgtttttg tccggcgggg aaacacacag aacagcccct gcggggcctt
     3481 tgtgtccgag agctttgccg ccacggcgtc gctcaccaga aaatgcctgc cgcccagggc
     3541 ggcaagctcc gggtgcgcat ccagcagctt ttgggtgcag tagacctcct ccggcggcag
     3601 acgctgggca aggtccgtgc acaggcgcag cccctcggca agaaagcgcc cctcggccgc
     3661 gcgccgcgcg ccgctgtccc gcagcttgca ggcgcgcttc acgcgctcgt tgtcgcggct
     3721 ggcgatcact tccgtcatgg cgcacaccct ccttttttaa tatccccgcc tcaggcgtgt
     3781 tgacgtaaga aaacacgcct gaggcggacc gttttggggc ctgcaaagcg ttttttcaca
     3841 caggccaaaa tacaaagcga cgcccgtgca cgttgtgccc gggcgtatcg ctgtgcttat
     3901 ttcgcggcgt tcttcgcggt ctcgacaagg gcggagaagc tcttcggatc atggatggcc
     3961 atttcggaga gcattttgcg gttcagctca atgccgctct tcttcaggcc gttcatgaag
     4021 gtggaatagt tgacgccctc ggcgcggcag gccgcgttga tgcgggtgat ccacaggctg
     4081 cggaaatcgc gctttttctg tttgcggccc acaaaagcat agttgccgct cttcatgacc
     4141 tgctctttgg ccattttaaa atgcttggat ttgctgcccc agtatccctt cgccagcttc
     4201 agggtctttt ttcttctttt gcgcgtcatc atagcgcctt tgatacgtgc catatttcac
     4261 aatctccttt aaatgtaagc tgtttgataa tcggttgcca agcccctgac agcttaggcg
     4321 tacggcagca tgcgtttgat tacggccgta ttcgtcttgt cggcatagga agccttgcgg
     4381 taggcgcgtt tcagcttgcg ggtcttgcca tggccgttca gcaaatggct gcgg
//
LOCUS       DAWBSB010000184         4378 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_3450_length_4378_cov_3.546842, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000184 DAWBSB010000000
VERSION     DAWBSB010000184.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 4378)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4378)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4378
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3450_length_4378_cov_3.546842"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1261..1270
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          963 a         1283 c         1305 g          817 t
ORIGIN      
        1 gctttgggcg gaacaagccg ccagctgctt tgaaaagcct gtttgtgtgc attggatgga
       61 aaggggggca tcatgaaaaa cgatatcgac gatctgctga acagcgtgtt cggttcgcgc
      121 acactgcgca ccggcgggca ggcggcaggc cgggagaagc cggaaacgcc acgtctgacg
      181 ctggagcctg aaccgaaaca gccgtccccg cgcggccgga gccctcagcg ccgcaccgcg
      241 ccgggacggc ccgcggcacc ctcccgcggc gcctcgcggg aggacgcgcg ccccaaggcg
      301 gacgtaccag tcattgactt tgacgccatc ggaaagcagc cccccgggac agatctccac
      361 gccgcgccag actttgccgc catagaacgc cgggctgcaa acgccgcgcg ggagctgcag
      421 gctctggagc gcgcagctcc acgcacgggc gcggcgctgg acgaggccat cgcgcgccag
      481 cgaaaggaga tcgaggccct gaaccgcagc gcgctggagg acatccaccg catcgggcgt
      541 gagctggaga ctcctgaagt gcgcggcctt tccggcgggt ccgcacaaga ggcggccgcg
      601 gctcccctgc ccaaccacgc cgccccgcgg ggcaaggcgg ggctggaggg cttcgacggg
      661 ttggccgaag cgctgggcgg aaccgtactg ggtcagccgg attatcttca aaagctggtc
      721 atcgcgctca agcggccgta tgtgatgggc cacgagggtg aaagcgcgcg caacgctttc
      781 ctcgtcaccg gtccagcgga cacaggcaag cacctgagtc tgtgtgccgc cgcagaggaa
      841 ctggcccggc gcggcgtgtt cgtcagcggg gatatctcct ggatggatct gtcgctgtac
      901 ccaaccgccg cagaggaaaa gctttttttg caggatctgt atatggcgct ggccgccaag
      961 ggcgacatcg tagtgttcga acactacgag cgctgccagc ctgcgttcct gacggtcctg
     1021 tccaatctgg tgcagcgggg caaaagcccg ctggccgggc gctacgtatt gcagaacggc
     1081 cgtctggtgg acgcgggcaa cgcccttgtc accgacgccg tgggcgcgct cacgccacgg
     1141 ggcaaatacc ttgtgctgct cagcgaggcc cccgttgcca agctggcgga cagcttcggc
     1201 gcgccgtttg tttcggcgct gggagatatc tgtgagacgg ccgccctcac gcccgaaagc
     1261 nnnnnnnnnn cccgaaagct tgtccgctgt ggcgaaagcg gaatttgaca agctggctgc
     1321 ccgcgcgcaa aaggcgctgg gcttcaccgt cacagccggg gatgcggcac tgctcctcgc
     1381 cgccgaaagc agcggacgcg ccaagggcgc gcagggcgtg ctggcattct gggaccgcgc
     1441 ctaccgcgcg ctggcccagt accggctgga acacgacgac gcacccaaag ccgccgcgct
     1501 gggcgcagcg gacggccgcc tgactgcaga cttcggcgca ggcccggcgg atctgctctc
     1561 cctgcttcct gaagcttacc aaggcgaggt ggaagctgta aaggcagaat tggacggcat
     1621 cgtaggcctt gcagccgtaa aggaatacgt actcagtttg gaggacaatt ttgccgtgca
     1681 ggcgcggcgc aaagccgccg gtctcaagac ggccagcgtt tccatgcata tgatcttcac
     1741 cgggaacccc ggcacaggca aaacgaccat tgcccggctg gtgagccgtt acctgaaggc
     1801 catcggggtg ctgagcggcg ggcagctggt ggaagtgaca cgcgccgacc ttgtgggccg
     1861 ttatgtgggc cacaccgcac cgcttaccac acaggtgctc aaatccgccg tgggcggcgt
     1921 gctgttcatc gacgaagcat acagcctgta ccgcggaaaa gacgacagct tcgggctgga
     1981 ggccattgat acgctggtca aaggtatgga ggaccaccgg gacgatctca tcgttatcct
     2041 ggccggctat tccaaagaga tggccgaatt tttgacggcc aacagcggcc tgaaaagccg
     2101 ttttccaaac atcattgaat tccccgatta taccggcgaa gagctgctgg ccattgcaaa
     2161 gctgcaggcg ggcggcaagg ggtatacgct ggacgagcgc tgcgacgcaa atctgctggc
     2221 ctatttcaac gccgtgcagc tcacccgcgc gcgggacgca ggcaacgggc ggctggcccg
     2281 caacaaagtg gaggaggcca tcctcaacca gtcccgccgc cttgtggccc agcccgaagc
     2341 agacctttgc ctgctgctgc ccgaggattt ccagttggac gatgtgggcg gagtataaac
     2401 agccttccgc aggtctgcct gcgtttttat agatatctga caagcaaagg agatgttcac
     2461 tatgcgaaaa tatcaggctc tttttttcgc aataagacca ggagggtcct gcaatgctga
     2521 cccttcgcac caagggaggc tgtcatgata aaattgacgc agatagacga aacaaatttt
     2581 ctggaatgct ttgcgctacg gctgggcaat gggcaggagc gctttgtctc atcccccatc
     2641 cgcagtctgg cacaggctta tgtgtaccgg gaccagtgta tgcccttcgg catttacgcc
     2701 ggggacaaga tgatcggata cgtcatggtt atttacgacc gtgaggaaca gacctacaat
     2761 atctggcatt tcatgatcga cgcggcgcac cagcgaaagg ggtacggcaa agctgctttg
     2821 gcgcaggtga tccgatatat caaaacaaag ccgtttgggc cgtccggcac ggtgctgctc
     2881 acctgcagcc cggaaaacca ggcagcgtac gccctgtata cagattttgg cttttgccca
     2941 acgggacggt gcgacggaaa ggaacaggag ctttgcctga agctcgcccc ggcgcggccg
     3001 aatacggaga taaaagtata atacgccaac agaaaacgcg ccccgcaggc cggatacatt
     3061 tcggatgcgg gcgcgttttt attgtagaga aaccaccggt tttgcatatg cgtgcaaagc
     3121 cctgtattac cggtgttgct tttcaaaaag aatgagaaaa gttttcagaa agaatgagaa
     3181 aagcatgttg caaaattgca acaaaaaggc aaaaagaaaa gggcgtttac aacgggttaa
     3241 aaagcccatg taaacgccct tttaaagtgt gtaaaaacga ttgtttacgg gtgtgaatga
     3301 ggcagcatgc agcgctttca ttgcaccctt ttgtcatgga ttcggaggca cgccgctgat
     3361 gtgtaccgca gctttgtttg cgggatctgc cgcttcaacg atatcctcca gaacgatctc
     3421 cccaatgtcc gggttttgaa gccttttgat gaggtcgctt gcatagttcg aaccgcggga
     3481 aatgacgatc cccgtgagca gagtacccag ccagtgaacg gagaaagaaa tacccataag
     3541 ggcaaagata tcagccccag cggcgaagca gataaaaact gcaatgagga tagcagccaa
     3601 ctgcgttccg aaggttttgt actgtttgtt ctcaagcatc gtaatgatgg tcttagcgta
     3661 ttggaccaga gcctcgacga tgattgcaag agacaggata agaagaaaag cctgcataaa
     3721 aatgtcctcc ttaatgaaaa aatatacaca gaaatgaaag aacacagccg atgctagacg
     3781 cctgcaacat acgccttaat atcagacagt tttttgtccg aaacatccgc gcgctgtgtg
     3841 gccgcgtcac gctcttttgt cagctgcgca acctgcgctt ccagttcgga tgtatcacca
     3901 tcaccagcct gcgccataca tgccgtaata gcatcccctg cggacaggct cacgatttcg
     3961 ctgcgatcag ggagcaccac ggcataacgc tttgctgtgc cagcctggat gcgcacccag
     4021 gtataagtgc cgtcgctgcc cacctcagcc tgcaccgggt aatattcgcc cactttcaag
     4081 cggccaccgt tgtatcccac atcgacagcg gttacatccg cagccgagaa tacctcgcac
     4141 ttttccgaaa gtaccttcaa aaatttcatg tcgtcatcct ccgtcccgtt gattgctgca
     4201 ccatatgtgc ccacagcatt ggggtgcccg gtgtatgccg tggggtcaag ccccgccccg
     4261 gtggtcgtgg cccgcacctc aaaatggcag tgtgcaaagg gcgggcttgc cagcgccgcg
     4321 ttgcccgtgt tgcccatcac cgccagcgcg tcgccgcttt tcacccgctg ccccacgg
//
LOCUS       DAWBSB010000185         4365 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_3458_length_4365_cov_3.099768, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000185 DAWBSB010000000
VERSION     DAWBSB010000185.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 4365)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4365)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4365
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3458_length_4365_cov_3.099768"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          862 a         1529 c         1110 g          864 t
ORIGIN      
        1 acttttactt caccgcaggc ggtatccaaa gcgcaaacgt aaaaatcctc accgggcgtc
       61 agctcccgct ccacatcaaa atcacaggta tgcactttgg cgtaaacgag ctttctctct
      121 ccgaacctgt caaaaagaac aagcgtattg cgcgggcctc ccgcatattc ttccagcagc
      181 gtgacgccga tggccatttc cagctccccg gcaagccgct ggaaggaacg gacaaagctg
      241 ccgccggacg gaacagcctc agccttccac tcctccgcgg gccgtccgta aatattgtat
      301 ccgttgctcc acatctcggg gaacaaagcg atatccgccc ccatcttctt cgctttccga
      361 caggcacgga ttcccttttc cagattctct tccaagccgc cgcagggcgc tatctgcaat
      421 aaggcaattt tcaaaggccg catatatgcc tctccccctt taccgcccgc acagcgtgcg
      481 aacgcgttcc gcacacagca gcattccggc cacgcccgcc agcaggaacg ccggcagcag
      541 accgatgccc agccgtgcgg ccagcacgcc gaacagagcg ggcacaaatg tggtgcccac
      601 atatgcgcat gccatctgca ggccaatgac cgcctgggaa cgctccgccc caaagcggta
      661 aggcgtttca tggatcatac tcgggtacac gggcgcaaag cccagcccaa gcacggccat
      721 tgccgccacg ccgaacccgg caggcagcgg cagcatcagc agtacggcgc ccccggcgct
      781 gacgagctgg cccagccgga tgagcgccgc gcttttcata cgcatactgg caaacccgga
      841 aaggaaccgt cccaaagtga tgcccagaaa gtacagcgac acgccccggg ccgccgcctc
      901 tgctgaaagc ccccgtgctt ccaccaggta gctggcgccc caaaggcccg cggccgcctc
      961 gatggcacaa tagaaaaaga atgtcgccag cgcgcctttc acgcccggaa tgttcagcgt
     1021 gctttgtttt ttgcccgcct gcttcgcggc cgcggcctcg gcccgcagag ccgcccggtc
     1081 ctcatatctg tcccacatgg gcagcgtgac ggccagcacc gccaccagac agaactggat
     1141 ggcacacacc gtgccgtaac cgccgcgcca gccatgcttc ccgcccagga acagcgatag
     1201 gataacaggc cccagtgtag ctcccacgcc ccaaaagcag tgcagccagc tcatgtggcg
     1261 cgcctcgtag tgcagcgcca caaagttgtt cagccccgcg tccaccgcgc ccgcgcccag
     1321 gcccattggc accgccgcca gcgcaagcca ccagaagccc ggcgcaagcg catagcccag
     1381 cagtgcgcag gccgtcattg tcacacttac cagcgttaca cggccggtgc cgaagcggtg
     1441 cagcatcctg tccgccgaaa ggctggccac caccgtgccg cccgccacga tcattgaaag
     1501 cacgcccgca ctgtccagcg gtacgcccag cgcgggctgc atggcgggcc acgccgcgcc
     1561 cagcagcgaa tccggcaagc ccaggctgat gaatgccgcg tagatcatac aaagaaaaaa
     1621 cgtcgccata gatccggtcc cttctgcccc atcggggcga tgtcctgtgc atgcgccggg
     1681 ctaagcctac ggcgcggttt aaaactccgc aagctccgta aatgcactgt cctccgcttc
     1741 ggtgacgctc ccatagatgc accgggtcct gcagcccaca cgcagataat ggcgcaaaac
     1801 ggtctttgcc gtgaaaggca tctcccgctt cagcacgctg tcatacggca cccactccag
     1861 cctgccctca tcacatgtgc ctctcactgc ggcgccgggc ttcagccctg caaaaaaata
     1921 gtaattctgc cggatctccc cgtttttcat gcgcagcgtg atataccgca gacgcagctc
     1981 ctccaggctt tcctccgtaa ggccggtctc ttcccgcagc tcccgcagag cacaggcccg
     2041 agggtcgttc aattcggcct gctcaaaatg gccgcctacg ccgcaccagg acggcgctac
     2101 cacgcgggag ccgacccggt acagcatcag cagtttcccc tcccgaaaaa ggtataccgc
     2161 agtcatattc cgcagcttct cagccatggt gtttccctcc gtccggcgcg ctgctgcacg
     2221 ccaatgtttc cagtataaaa cgggccgccc gttttttcaa gccggacagc ctgtttcatc
     2281 ttggaaaaaa aacgcttttt cgccaaggtg cttccgaaca ggcgtttggc cccgttcgcc
     2341 gcgcacggcg gtcttcgccg cagcttcgct ttaaacgccc cgcgccggcc cgcggggcgc
     2401 acagcattgt ttgaaatgaa aaaggggaca gctgagcgct aagggccgca tcccctttcg
     2461 gcgcggttgt ccgcaagggg actttgcacg cttctatacg cgcctgcggg cgcagttccc
     2521 cttttggccg catccgcccc atggcctgca ctttgcttcg caaggctgca tggctttgcc
     2581 gccgccagct ttttctccgg aagctctttt accttttcac ttttttcggc attccgacag
     2641 gccgcccgtt tctttatcgc ctcggcaaaa acggaatgcc gcagcgcttc acgccgcccc
     2701 caggctcagc agccaccgca gcgcgggaca gagtcccgga caattcgccg tcagatacgc
     2761 cgccacatcc tgcatcttcg tcgcttcggt gataaccagg cccgcatctg cattcgcccc
     2821 cacagagaca ggcgccgcag ctgcgccgcc cacggccacc cgggaagcaa cggccgcgcc
     2881 gcccatggcg tacataccaa tggcacagcc gcctgccgcc accagcccca cggccacgcc
     2941 gcccacagcg aacccgccca gcgcaaggcc gcccaggctc agcaggccca caccgatgcc
     3001 gcagaagctt acaacgccca cgccaaatcc ccccagcgcg acgatgcccg tggcaatatt
     3061 gccgatggcg atgacgcctt ttgcacgccg caggccatag ccgcagttga tgtgcaccag
     3121 tggaaggccg agcagtgtgt gtttgctttt gtattcataa ccgggaacaa cgttcaccgt
     3181 cgcgcccgcc ggcacacaca ccgtctgtcc tgccgtcacc acctgcaccg taccgccggg
     3241 cttcgtttcc accacggcct ccggccgcac caacgcgtcc agtgaagtac cgaacaggtc
     3301 cgccagagcc accagcttgg gcagatccgg catggtctgc cccgcctccc atttgctcac
     3361 cgcctgacgg gaaacatcca tcaagtctgc aagctgttcc tgggaaaagc ctttctcccg
     3421 ccgcagccgc acaaggttat ccgcaaaggt catattgatc tctccttttg tttttgatgg
     3481 acttatcata gcctgttccc ctttctgtgc gccaccaacc tgcgccttgc aatttgtcaa
     3541 ccgcaggttg cgggtgcttt ttctgctctt cacaaagcca aaaagggccg cgccgtggcg
     3601 cggcccgcaa aacaagcggg gagtgcggtc agcggttcat ctcgctcacc gggcccgtgg
     3661 cgttgtgctc cagacgtcca gtcttcatac agccgtgcaa acagcctgtc gacttcctcg
     3721 cagcgttttc ggctcgagcc tgttcgcttt gcatctgtcc tatctctgct gcctgctcac
     3781 tcccgaggtt tttctgcagc tcgcatatcc gctgtcctcg atccaattgc gcctgttcca
     3841 tccaatgctg taaccatgcc agcaaataaa catacaacat atcacagcga atgtagggtt
     3901 taaagtatct gcccgtaccg tactgtgaat aacaggtgca actgaagtaa cgattccgta
     3961 ttttgttttg ccgttcgtcg caaacttcat tgaccatcca cagtccgcac aacgtaccag
     4021 tccggcaaat atttgtgtgg ttgcgttctt ttgtttcctt cgccgggagg cgatttgctg
     4081 ctgtacacgc tcaaagtcct ctttggaaat gattgcttcc tgggttccct cgacacgcat
     4141 ccattcactc tgcggcttgc gtatccgctt tttgtttttg tatgagatat tcgtctcacg
     4201 gtagtggatg gtgttgccga tgtacgtctc atccttgagg atgctcttga cctgtgcaat
     4261 tgtccaggca taccgctttt cctccggagc gtccctgtag atattggcaa atgtcccgtc
     4321 gcgctgataa ttcagccaac cgggtgtggg aaccttctcc tgcgt
//
LOCUS       DAWBSB010000186         4319 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_3499_length_4319_cov_2.623358, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000186 DAWBSB010000000
VERSION     DAWBSB010000186.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 4319)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4319)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4319
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3499_length_4319_cov_2.623358"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    264..273
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3831..3840
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1024 a         1163 c         1250 g          862 t
ORIGIN      
        1 acacaactga acagagaaag tgagagaaca ggaatggaac cctctaaagt atattttaca
       61 gacatgcgtg cgctgccggg cacgaacctg cagcagaaac tcaagcggct gatcctcaaa
      121 gcgggcatcg gacagatcga ttttcaggat aaatatgccg ctatcaaaat ccatttcggc
      181 gaaccgggca acctttctta cctgcggccc aattttgcaa agaccgttgc ggacgccgtg
      241 taagagctgg gcggccgccc gttnnnnnnn nnnccgtttc tggcagactg caacacgctg
      301 tatgtggggc gccgcaaaaa tgcgctggac catatcgaag cggcttatga aaacgggttt
      361 tcgccatttt ccaccggctg ccagatcatt attgcagacg gcctgaaggg gacggatgag
      421 gtggacgtgc ccgtgcgcgg cggcgaatac atccgggagg cccatatcgg ccgcgccgtg
      481 atggatgcgg atatattcat ttcgctgtcc cacttcaaag ggcacgagag cacgggcttc
      541 ggcggcgcca tcaagaatat cggcatgggc tgcggttcgc gcgcaggaaa gatggacatg
      601 cattgcgcgg gtaagccgga cgtggacaaa agcgtttgcc ggggctgcgg cgtgtgcatg
      661 aaaaactgcg cgcacggagc cattgctttt gatgaaaacc gcaaagcggg catcgaccac
      721 ggcaaatgtg tgggctgcgg ccgctgcatc ggtgcatgca attttgacgc cgtatacaac
      781 ggcaacgatt ccgccaacga cgagctgaac tgcaagatgg ccgagtacac caaggctgtg
      841 gttgacggcc gcccggcttt ccatatcaat atcgtcaacc aggtttcgcc ttactgcgac
      901 tgccacggtg aaaatgacac ggctatcatt ccggacgtgg ggatgttcgc cagctttgac
      961 cctgtggcgc tggacgtagc gtgcgcggac gcgtgcaacc gcatgcccgt gatgcccgga
     1021 agcctgctgg acgaaaacct gcgcgcctgc ggcgcccgtg accatcacga ccattttacg
     1081 aatgtttctc ccaatacaga ttggcgctcg tgtatggagc atggggaaaa aatcggtttg
     1141 ggcacacgca gctatgaact gattacagtc aaatgaagaa gcttttgagc ggcggggata
     1201 cccgccgctt ttttatgcag agcttctttc tgcactttgt acagaaaaca gtttgaatta
     1261 gcatgaaaat gtcagttttt atttaaaata tgcaacaaat atccattttt aaggataaaa
     1321 atatattttt cattttttga gtttataatc taataatgaa aatttttacc ttattataga
     1381 aaaatatgga aacttatttg ttttataatt atgaaaaaaa gatatcaaaa caaatgattt
     1441 tgttgacttt ttacaattgt gatgataata tcaaaccgtt gcaggaagca gcatagcttc
     1501 cgcttcttca cattttgctg catggataat ccatgcagcg cggccttgcg cagatgcgcg
     1561 ggctctgctg cctgcggcgg cgggaggccc ggcggcgcac atgccgcatt gcaggaggct
     1621 ccggcggcgt gtggggaaaa atgcaaatga aaaggagaga aacgtatgaa aaaagtacta
     1681 gcgatccttc tggcggcgtg cctggcactt tctctggcgg catgcggcgg cagcggttct
     1741 tccgcgcctg tttccacagc gccggatacc tctggctccg cgccggcttc cgcgcctgcg
     1801 gcggccgacg gctccatcgc cgttttctat tacacgtaca gcgacacata tatttccacg
     1861 gtccgcaccg cgttggacgc ggagttggat aagctgggcg ttgagtacca ggactatgat
     1921 tccaactctc agcagaatac ccagaccgag caggttgata ctgccatcac gaaaggcgct
     1981 acctgcctga ttgtgaacat cgtcgagaca ggctccgatg acgcggcgca gggtatcgtg
     2041 gacaaggcga aggcagcgga catccccgtg atcttcttca accgcgaagt gtccgacgcc
     2101 gtggtgaaca gctatgaaaa atgtgcgttc atcggcactg acgcggccga ggccggccac
     2161 atgcagggcc agatgatcgc tgattatctg ctggcgaatt atgacgacgt ggacctgaac
     2221 ggtgacggca ccatctccta tgtcatgttt aagggccaga acgccaaccc cgaggccgaa
     2281 taccgcaccc agtatgccgt cgaggactgc gacgctgcgc tggaggccgc cggcaagccg
     2341 gcgctgacgt tctatgacgc caacaacgcc gacaagttcc tggtggaccg taacggcaca
     2401 tggtccgcgc aggcttccaa cgagtatatg acgacgattc tgtccgagta caccgaggcc
     2461 aataacaaca tggtcgagct ggtcatctgc aacaacgacg gcatggccga gggcgccatc
     2521 acagcgctca agaccgccgg ctacaacgcc agcgcggaca aggccatccc tgtgttcggc
     2581 gttgacgcca ccgatgcagc caaagagctg atcgccgccg gcagcatggt cggcagcatc
     2641 aagcaggacg ccgaaggcat ggcggcggcc attgcgctgc ttgccaagaa cgccaccagc
     2701 ggcgcggccc tgatggacgg caccgacagc tacaatgtgg atgatgccag tgccaagatc
     2761 cgcatcccgt acgccgttta caccggctga tagacccgat caagcaaaca gcaggaagat
     2821 agctgcctct acgggcggct atcttcctgc ttgaatctga gattttacgc ggaggagagg
     2881 ccgaatggag aacaacaaca gcgttctgct cgaaatgaaa aatatctgca agtccttccc
     2941 cggcgtcaag gcgctcgaca atgtttcgct gacggtaaag cgcggcacgg tgcatgcact
     3001 gatgggggaa aacggggcgg gcaagtccac cctgatgaag tgcctgttcg gcatttacag
     3061 caaggacagc gggcagatcc tgctgaatgg gaaggaagtc agcttcaaaa cctcgaaaga
     3121 ggcgctggaa cacggcgtcg ccatggtgca tcaggagctg aaccaggcac tcaagcgcaa
     3181 tgttatggac aatatctggc tcggccgcta tcccacggtg gggcccttca cgtccgaaaa
     3241 aaagatgctc gctgatacgg cggcgatttt tgatgatttg ggcatccatg tggacccgcg
     3301 caccattatg agcacgatgc ccgtggccca gcgccagatg gtggagattg ccaaagctgt
     3361 ctcgtaccat tccgacgtca tcgtgttcga cgagcccacc tcctcgctta ctgaggagga
     3421 agtggagcac ctgttccgca tcatcaatat gctgcgggac cgcggctgcg gcatcattta
     3481 tatttcccac aagatggagg agatcctgcg tatctccgac gaagtgaccg tcatgcgcga
     3541 tggcgcgtgg gtcgccacag agccggcggc ggaaatgacg atggagcgca tcatcaagct
     3601 gatggtgggc cgtgagctga caaaccgctt cccgcccaag gacaacgaga tcggcggcgt
     3661 gctgctggag gtggagggcc tgacggccat gtattccaag ctgcgcgatg tttccttcaa
     3721 tgtacataag ggcgagatcg tcggcctcgc cggcctggac ggctccggcc gcaccgaggt
     3781 gctggaaaat attttcggcg tggctacccg caaaagcgga gtcatccgcc nnnnnnnnnn
     3841 aagaaccgcc gtgcccgcga cgcgatcaaa aacaagttcg cgctgctcac cgaggagcgg
     3901 cgcgccacgg gcatctttgg cattttggac attcaggaga ataccaccat ctccagcttg
     3961 aagaaatata aggtaaaggg cctgtacctc agcagaaaga agatggagga gagaacggag
     4021 tggtccattc gggccatgcg catcaaaacg ccgtctcagg atacgaagat ccgttcgctt
     4081 tcgggcggca accagcagaa agtcatcctg ggccggtggc ttctgacaga ccccgaggtg
     4141 ctgctgctgg acgagcccac ccggggcatt gacgtaggtg caaagtacga gatctatcag
     4201 ctcatcatca acctcgccaa agagggcaag gccgttatca tggtgtcctc ggaaatgccg
     4261 gagcttctgg gcgtgtgcga ccgcatcctt gtaatgtccg gcgggcggct cgccggaga
//
LOCUS       DAWBSB010000187         4308 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_3511_length_4308_cov_3.393134, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000187 DAWBSB010000000
VERSION     DAWBSB010000187.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 4308)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4308)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4308
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3511_length_4308_cov_3.393134"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          780 a         1541 c         1232 g          755 t
ORIGIN      
        1 atatataagc gtgagccaag gcttcgcttg caagcatcgc cggcatttga gaaaacaatg
       61 cactaatctg ccctttttgg gccgacgtaa gctccagttt tgtaaggccg gattttgtat
      121 ccaagtcaaa atttttatcg cacggtgtca gttcaaagtt aaaatctttc tctaacgtcc
      181 gaaaaagttc cagctgctct gacatgctca tctacctcca gataaaataa aaagtacatc
      241 ccacaataag aaacgcgcca ccaaagcacg ttctcttatg gctaccgcac agaccttaca
      301 cgcctagccg taggtgcgat aaaaaaagaa gcactttttg ccaaagcatt tcccatggct
      361 atagaaaatc gtgtagcaac aatattatac cacactctct gcaataatag aatatttttc
      421 cggcattcat ttacattctt caaactgtca cagtttattg cttctcctgc tgtccacgcc
      481 atattaaggc ctcgacagta tccttttgca aaacttttac aacgcggagc gcctttcaac
      541 gcaaaaatca gaacacagat ggagcatagc cgccgcgcag ggtcaccgga accgctccgc
      601 gccccaccag tgcggcatca gctcccgcaa acgcaccgtt ttcccggttt cggcgtccgc
      661 cagtatttca aggccggggc tgtccttatc cagctgcatc atgaattccc tgcacgcgcc
      721 gcagggcatt cccacgcgcc cgtcccccat cacgcacacc agccgcacaa tgcggctctc
      781 cccgttcgtg atcatattgg cgatggcgct gcgctccgcg cacatgccca gcgtacaggc
      841 ggtgtcgatg cacaccccgg tgtaaacatt tcccgccgcg gtgagaatgg cggcggccac
      901 gccgccggcc tcgatgaacg gcgagaccgc gcggggggcc agcttctccg cggctttgtc
      961 ataaagctct ttccattgca tggtatgacc ctctcttttc tttttacagg ccgcccttgc
     1021 gccgcctccc aatattcccg cgggccgcgg ccgggcgcgc ggcgcgtcac tgcccgttca
     1081 gctcccgcac aaacgcctcg ggctttgccg tcagctcggc ccaggcaggc acaaagccgc
     1141 atttcacggc gttgcgcacc gatttgatgt ttgaccacgc ggcgcagtaa aacggcacct
     1201 ttccccgggc aagcgtttcg gccgcaaggc gggacgtcag cgcggcggca acgccccggc
     1261 ggcggtatcc gggcagcacg tccacgccga tctgccacat gctttcacaa tccgccgaac
     1321 agccggccag gcccaccagc cttgcgccgt cgtacgcgcc cacggccagc acgtccaatt
     1381 ccctccgttt ttcgcacaga gcgttgctcc attccggccg gtacaggccc gcgaagtccg
     1441 caggccccag caccttcagc gcatacccgc aatcccactg ccgcatgcgg gaaacatccg
     1501 gcagaaaata ttccgccatg aagcatatcc gcatgccgaa gggacgcagc gcgtccgcca
     1561 gcacgttcat gttcggcgtt tcaaagcaat gctcgggcgc ataccgctcc acataggccc
     1621 gcacggctgc ctcggcctcg ggcgcgacgg aagccaccac gttgccgccg taggagacaa
     1681 ggtcgcagaa aaagggcagc gacagatatt tccgcgcccc ggcatgaggc gcggaacgca
     1741 ccaccacgct gtgctcccgg ccgaagtcct ccgggctgca attcagatca acggcggact
     1801 gccgcagcgc ggcggcccat atttcctctt ttgtcatata cgtcatccct tccggctccg
     1861 gccgcgctcc gcccgcaccg tcccgccgcg cgaaaacagc ctgccgttgg ccagcgcaag
     1921 caatgcttcg gccgcggggt agcggatgat gccgctggtg ttgagcacca ggtttttaaa
     1981 cggcaaatcg cgcaccgtca tctcgcacac ccggcggttc accgccgggt tgttggaaaa
     2041 atgcatgccc agccgcgccg cgcacaaagc cagcgcgtgc agcgcgcgcc ccgtgcggct
     2101 gtccagcatg tcgcccagca ccgtcgtctg ggtgtattcg ccgcggacgg gcttttggtt
     2161 gcgtggcagc ggccgcgtgt ggatggcggc aaacgccgcg tccgggaacg tgttgtccgc
     2221 aaacgagccg tacggccccc cggcgctgtg ggcgggcggc agcgccaccg tttcgccccg
     2281 cgccgtaaaa gcggcgtgca gcagcaaaca gcggctgtgc cgccccacgg acacctcata
     2341 acggcccgcc tccaccacga acgcatggcg cgccacatca taaaactcca gcgcgctggg
     2401 cggcagcgtc acacaaaccg ttttgcgctc gcccggcttc agcgccacac gggcaaagcc
     2461 cgccagcgtg cgcacgggct ggtgcgcggc ggaattccgg tgggccacgt acacctgcgc
     2521 ggtctccacg cccgccatcg cgcccgtatt ttccaccgtg aaaagcacct cgatggcctt
     2581 gcccggcgcc agcaccttgc gggaaagcgt aaggcccgaa tacgcaaagg acgtatagct
     2641 cagcccgtag ccgaagggaa agcgcacgtc cacatgcgct ttgtcgtaat agcggtagcc
     2701 cacataaatg ctttcgttgt aggtaacggc gtccggcccc atgggccagt ggcgcgcggc
     2761 gggcgtgtcc tccagccttt tgggccacgt ctccgccagc ctgcccgccg ggcatgccct
     2821 gccgtacaaa agctggaaca cggcttcgcc cacgccctcg ccgcccagcg ccgtgtacag
     2881 cacggccttc gccctgggca gccacggcgt ttccacgggg ctgccgcccg ccagcaccac
     2941 caccacgtct tttcccgccc cggccaggcg gtccagcagg tgcagctggt tgaacggcag
     3001 gcgcatgtgg gcgcggtcgt agccttcggt ctcgaacagg tccgtcaggc ccaggaacag
     3061 cagcaccgtg tccgcgcccc gggcggcgtt cagcgccttt tcctccagct ttgcgtccac
     3121 gcattcgctg tccgggtcat agccgggcgc atacgcggcg tctctgcagc cgccgcgcac
     3181 cagcgcgtgc aggaaatccg gcctgccgtg ggcgttgatg aggctggagc ccgcgccctg
     3241 ataccggcag cgcctgcccg ccagcgcgcc gacgaccgcc accttcgcgt ccggcgccag
     3301 cggcagcacg cggccttcat tcttcagcag cacggcgctc tcccgcgcgg cgcgcacggc
     3361 cagcgcgtgg tgcctgtcca gcatgcggcg caccgccgcc acatggtgca cgggcgcggg
     3421 atacgccttt ttgcgctgcg gcgcatgggc cagcgccagg cgcagcaccc gggcgcacag
     3481 cgcgtccagc gccgcctcgt ccagcctgcc cgcgcgcacg gcccgcacga tctcccggtc
     3541 cgtcacgccg ccggaggagg gcatttccag gtccagcccc gcggccacgc ccgccgggcg
     3601 gcggtatacc gcgccccagt cgctcatcac aaggccgtca aagccccact gctcccgcag
     3661 gatgcgggtg agcagcatgc ggttctcact ggcataaacg ccgttgatgc ggttgtagct
     3721 gcacatcacg gtggagggcg cgccctcctt caccgctatt tcaaacggcc ggagataaag
     3781 ttcgtgcagc gcccgctcgt ccacctcggc ggagacgctc atgcgcagcg tctcctgatt
     3841 gttcacggca aaatgcttga ggctggtgcc gacgccctcg ccctcggccc cgcggatgaa
     3901 cccggcggcc agcttgcccg ccagcagcgg gtcctcggaa tagtattcaa agttgcgccc
     3961 gcacagcggg ctgcgcttga tgttgacgcc cggccccaaa acaacggcca cgccgtgggc
     4021 cctgcactcc cggccgatgg ccgcgcccac cctgcgcgcc agcgcggggc tccacgcgca
     4081 ggccagcgcc gacgcagtgg gaaagcacgt tgctttcacg ctgtggccgc cgttctcacg
     4141 gatgtgcaga cggtcctcct tgcgcaggcc gtgcgggccg tccgccagcc atatggaagg
     4201 cacgcccttg gccgggatgt cctgcgtctc ccaaaagctc tttccgctgg ccagggatgc
     4261 tttttcctcc agcgtcagcg aagcgatgat gttcccgatc cgatcatt
//
LOCUS       DAWBSB010000188         4288 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_3530_length_4288_cov_3.120246, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000188 DAWBSB010000000
VERSION     DAWBSB010000188.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 4288)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4288)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4288
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3530_length_4288_cov_3.120246"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3279..3288
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          899 a         1136 c         1288 g          955 t
ORIGIN      
        1 gcatgtggtg tctgtcacgg cggactgcga cggcgacgcg ctggtggtgg aagtcattaa
       61 ggacggtccc gcctgccatc tgggcacgga ttcctgcttt gccaatctgc ttttccggga
      121 cgaggaggtg cagcggtttt cactggaggc gctgtacgcg ctgctggagg gccgcaatgc
      181 ccagcgtccc gagggcagct ataccaccta cctgtttgaa aaagggcggg aaaaaatctt
      241 gaaaaaggtg ggtgaggaat gcaccgaagt catcatcggg gccatgaagg gcagccggga
      301 ggaaaccatc tacgaaattt ccgacctgtg ttaccatgtg ctggtcctga tggtcgcaga
      361 gggcattgcg ctggatgaca tccgggccga gctggcgtcg cgccatgtca ttgaccataa
      421 ggtgaaacag gagacgatgc aatgagactg acatcctcca cacacaacca tacaaatctg
      481 tgcgacggca ggaacacgcc cgatgaaatg gcccgggccg cgttggaggc cggctttacg
      541 gatttcggct tttccggcca cagctatgcg ccgtttgatc tggattattc cgtcaaatcg
      601 gagcaacact atgtgcagga gctgcgtgcg ctgcaaaagg catacgcggg ccgtctgcgc
      661 attgccgtgg gaatggaagc tgattggttc gctccggtga acgaccgcgc ggcgctggac
      721 tacatcattg gctctgtgca ttacctgcgg gacgaagcca caggccggta ttatgccgtg
      781 gacggcgcac cggaggctct ggacgcctgt gtgacgcaga tgttcggcgg cgacgcgctg
      841 gccatggccc gggcctatta cgcgcttgtg gctgaaaacg tccggaaata ccggccggag
      901 atcatcggcc atttcgacct ggtgaaaaaa aacaactgcg gcggacacct gtttgatgaa
      961 gcggacccgg tttaccgtac tgcggcgctg gaggcgctgg acgcctgcgc cgccaccggg
     1021 gctgtttttg aggtgaacac gggcggcatg ttccgcggtt actgcggtga gccgtatccc
     1081 tcgcgctttc tgctggaagc cctgcggaaa agcggcgcac gcgtcactgt aaacgccgac
     1141 gcgcactgca ccgaagccgt gcgtttccgt tttgacgaaa cgctggcgct gctgcgggat
     1201 atcggcttta cggctgtgac ggtgctggag gacggcatgt ttgtggaaaa accgctgtga
     1261 cgcagccggt gcgtgaacat cctgacgaaa tccaatggct tgatttgtgc gtatcgtaca
     1321 gaattttgtt gccgtgccgg gaaatgcttt gcaccggtgc gccgttcttt tgtttgcacc
     1381 cgcgcgcggc ggatggtata atgcttgcgt gaaacggagc tcttgcggta ttccgcggcg
     1441 cggaagtatg cggggcggga ggccccggcc cgcacagaag aaaggacacg aatatgacga
     1501 acatctggca tgatatcagc ccatcgcgca ttaacccgga agacttcatc gcggtgatcg
     1561 aaattgaaaa aggctcaaag aaaaagtacg agctggataa ggagaccggc catatcattt
     1621 tggaccgcat cctttatacc tctacccact atccggcaaa ctacggtctg atcccgcgca
     1681 cctttgcgga cgacggcgac ccgctggacg tgctggtgct gtgcagcgag gtgcttgcgc
     1741 cgctgagcct ggtacggtgc tatcccatcg gcgtcatcac catgcaggac caggggaaaa
     1801 tggacgagaa gatcattgcg atcccctttg aggacccaac ctacaacacc taccgtgaca
     1861 tatcggagct gcccagccat atcttcagcg agatgcgcca tttcttcagt gtgtataaga
     1921 atctggaagg caagaccacg gtggtggacg aggaacaggg gccggatgag gcgaaacgca
     1981 tcattcagaa ctgtctggac aattatatcg aaaaattctg ccgctgaacc cgacaaaatg
     2041 catgccccgg cgctgcccgc gccgcgggct tttttgtgct ttcttggatt tatttgcgct
     2101 gtgcggcggg cgggattgac agaccaaaga catacactta tacttttata acaaacttat
     2161 aaaagagaat aacgaaagag gtgccgctgg tggaaaactc ccatgcaagc ccgggcctgc
     2221 tgaagcaggc caacagctcc gcgttgcaca gagtgctgcg ggaaaaaggc gcggctaccc
     2281 gcgcgcagct tgctgcagca gcgggcgtaa gcgtcacgac ggtgcgcgcg ctgctggagg
     2341 agatgctgcg ggacggcgag gtggaatgca ccggcctgag cgcctccagt ggcggccgca
     2401 gagcggcgca atacagtctg cgcagggaca aatactatgg tgcggccgtg tgtatcacag
     2461 acgggcaggc tcaaggcctg ttggtggatg tgcacggaga gattgtccgg gccgttcctc
     2521 tgaaggccgg agcagacggc ctgcgaacgc ccgtgctggc gtttttggat ggcctgttcg
     2581 cgcaaagaga tatccgctcc attggcttgg gactgcccgg tgtggtgcgg gaaggatgct
     2641 tttggcatgc ggataaagaa ggcggactgc acagtgtggc ggtggagcct gctctttcgc
     2701 ggcgttacgg cgtgcccgtc gtgttggaaa acgacgtcaa cgcggccgcc atcgggttcg
     2761 cccggtgcta cgaaacggaa tttccgggcg aagacccggc tgaaacgaac atggcatatt
     2821 tgcattttga cagaggttgt gtcagcgcgg gttttgtggc ggggggccgc gtgattcgcg
     2881 gctgcggcca ttttgccggg gagctggggc tggttcccac agcggacggc aggcttctgg
     2941 acgaatgcct gaacgacccg caggacgggc gggaatacac agcccgggta ctggaggcgg
     3001 cggtgtgggt ctgcggcatc ctcaacccac gctatattgc tttgggcggc ccggcgctgc
     3061 gccgggaatg cattgggccg gtgaacgacg gcctgagtgc gctgctgccc ggccccatgt
     3121 gccctgaatt gctctatacg gcggatatat ggggtgatta ccagatcggc atggcatggc
     3181 tgacggcgga aaaaatattc cacatggtgc agatcatcaa gaaataaata tcccccggct
     3241 tcgccggggg tatttcagat gcgtgcaaag ccctatgann nnnnnnnntc atggacgcgc
     3301 gctgcattgc cggaatgcag ggcccccaaa gcagcgtatg gctctatatt tcccttgcgc
     3361 tgggcctgac gctgctggaa gccgccgtcc cgctgtttat tcagcgccgc cgcaaaagcg
     3421 cgttggcgta agaaaacatc ggttttggac cggacggttt gacgcgcagc gtcaaaaggc
     3481 gggccatgat tttggcctgc aagtgtgccg gacgaggcca tgcgcaaaaa tttttgtttc
     3541 cgggcgatgg cttcctgcgt caacacgctt aagccgggcg taaaatacaa taaatatccc
     3601 ccggcgaagc cgggggtatt tcagatgcgt gcaaagccct atgatacagg cagcgcctca
     3661 aggcgcataa cggtctgaca cttgcatagg attgttactt gttacccgca agcgggtcca
     3721 tgtattcctt taacgtcatc tgatctgtaa gttgatcttc tttcaactga ttttgaatgt
     3781 attccgcgat ctttttcgta ttctttcctg cggtgtccac gtagtaccct ctgcaccaga
     3841 atgatcgatt cccatatttg tacttcagat tcccgtgtcg ttgaaaaatc atcaaactgc
     3901 ttttcccttt gagaaatcct atgtatcccg ccacactcat tttcggtggg atttccacca
     3961 gcatgtggat gtgatccggg cacacttctg cttctactat ttttactcct ttccattcac
     4021 tgagctgtct caaaatccct cctatctctt ttcgattttc tccgtagaac agtttgcgcc
     4081 gatatttcgg cgcaaacaca atgtggtatt tgcaattcca ttttgtatgc gctaaactat
     4141 tattgtcatt cactgaatgg cctccttctg ttggttcgtg cagttgccag accgcacttc
     4201 cattttactg gaaggagttt cttttcatag cttaagcttt tccgtaccac cggcacagcc
     4261 ggtggtttct ctacaataaa aagaggcg
//
LOCUS       DAWBSB010000189         4283 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_3537_length_4283_cov_2.807474, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000189 DAWBSB010000000
VERSION     DAWBSB010000189.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 4283)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4283)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4283
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3537_length_4283_cov_2.807474"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3221..3230
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          951 a         1113 c          994 g         1215 t
ORIGIN      
        1 gcgtcaggct atgccgagcg tagtatcgtt cctgtacatg tttcggaagt gtcgaaagcg
       61 gaaaaatgta ttccggtcga tttttggcat tgctttgaat ttcacacgtt aaactcccgg
      121 attttgcgcg tctctgtaca gtgcgtatgt caactcccat cagagaagcg acttgacttg
      181 gtttgagcat cgtttccaaa aaaactcacc tacctttact ccaacgacct gccgtcgtca
      241 ggcgcggggc ggtcagcccc cgcacgacag ccccacaggg ctgtttcggc tattcttctg
      301 tgtccggtat tgtccggctg aacgggatgc cgaactcgca gcgcagaaca tccacgcggt
      361 cgcgggatac cagtatctca gttgtcaggc cgcaatgggc ttccaggtac ctgcgcagcg
      421 gctccgcagc ctgcatcagt tcttcgtagt cgttcatgac aatttttcgt ccttcctggc
      481 taggtcatgg aggagacgct gggtctcctt cgccattttg agagagctat tgtacatctt
      541 aaggattttc acaagctcat ccatgatctc cgcgccgctc tttccattga aaacttccac
      601 cggagcgccc gcagcttcaa ggtcacgcat cagccgttcc accatcttgc ctctgctgtt
      661 catcggcccg ccgcgccagc acggccgcac cttctcaccg tcgtctatca tcagcccatc
      721 gggaatgcca tgttccagca cgcccttgcg cagtgcagcc agtgtctttt cgttggggat
      781 aaggtcttcc cgcagctgcc ggcgcatccg gacagcgaac tcatccgctg ttttctgcgc
      841 ggtatctgcg ccgaggggtt cttccatggg cttcaatgtg ccgcatagct cttggatctg
      901 ttcctcgatg aattcatcca cgttcgccgt agtaaaagaa acttccgtct ccatatcaag
      961 gatgagcttg ccctcatcaa ggcgtttcgc catttcacgg cacaggttct tctggaacgt
     1021 ttccacggcg ggatggatgc tcttctcaac gcctgtgagc ttgtctttgt tcatgttttt
     1081 atcctttctg gcctccgcgg cccgtttgtg gtattctgtt ggtgaggacg gcgttgcagc
     1141 accgtccagc accgtacgca agggagggat gtgttctaga aatctactta ttccatgtag
     1201 aagggaggtt gcaaaaatgc ttacaaaatc gcagattcgt gtgattcttg aagatgcttt
     1261 gaaaaatcct ggcttggaat accactctgg cgatcccgag atatacaatc atttggggga
     1321 tgtgcggccg tacgttttag agtatcaaac agtaatacgc ggctttaaat gcacggggct
     1381 ttcttcaaag ggaaaagccc ttttagatag ggattagttc acaggcacgc cggagagttc
     1441 aacttccttg atgtcgaact cttcggtttt ttctccgtct tcatagtagt gaatcgtaac
     1501 gattcccgtc tgcacatcta ccttgtacca agctgtgccc ggaaacgccc tgtcacctac
     1561 cagcggcatg ccaaatctcg cctcgatctg aacttcaccg tttccgacgc agcttccacg
     1621 cgtatttttg tgctgaccgt tcatagcctg ctcctttctg cccacattgc gtgagctttt
     1681 ttatttggcc tgccatcatc agtgccgggc ggccggtccc ggcagacggc ccaccgggcc
     1741 gtttcggctt ctatgttccg tagggcgtgt caagccctgg atgtattcca gggcgaccag
     1801 ctcgggaaag accgtgtgcg gaactcccgc acggctgcct ccgggtctgt tttcagcaca
     1861 aggccgaata caaggccgtt gctgtccaca tcgcccacgg cgtaagtgtg tgacgggttc
     1921 gccgccttgc tgcgaaggag cacgcagcgc tccgcgcgtt ttaccacatc ataagcggct
     1981 ttgaaattgc cttttaactc gctcatcggt acccctcctt cagaccgcct gctccagcgt
     2041 ggagcagaac tggtttactt ccctcggcca cagcagctgc acgcccgttg gcgtcaggaa
     2101 cagggccggg gcctctacat cgaagatcgt cccgtccttg atgcgtcgca gcgttacttt
     2161 cggctcggtg tacccttcgt cgaaactctc cacccggtac aggtccccgt tgtggttgta
     2221 aatctcgcca acatagatgg gttggccggt cagttgatac tttttcattg cattggctcc
     2281 ttcctgtttg aaagcccgtt tccgggcaat acttggcttg tagcagcgat ggacagttca
     2341 tagagtgcat ccgccagcgt atccgcccaa ccgatgtgtt gccccgccgg gccgctgacg
     2401 tagatgcggc ccacgcccgg atagtaccgg atgcggcacg gcgcccactt gggggaacat
     2461 gctgccgtca gttcgtgcct ccgcacttcc agaatgtcca ggctccggcc ggtccggcgc
     2521 ttcagttcct tccggcagtt gcttttcctt agctctaggt ggaagttcat taaagcgcct
     2581 cctggtcact tgtgaatatt tcttcaactt tgcactctaa agcgcttgcg attgccttgg
     2641 ccctcaaatc ttgggtgcgc gtatatgcac ccgtttcaat gcgaaaaact gcatttacag
     2701 gaagccctgc cttttcggat aatttctttt gattcaatcc acgagccttt cgcagttcaa
     2761 tgattttatc aacttttgcg ataagcattt gcccacctcc aacgtcaaaa agttttacgt
     2821 tcgtacaagg tacatttata taatatattt tacatacgta aaagtcaacc ctaaatttgt
     2881 tgaaattagc attttacgaa cgtaaaatta tatggtatac ttacctttaa tgacaggcag
     2941 gtgatatttc tatggacaag cccatcgcaa aatcaaataa ccgtgttcga gagctccgtt
     3001 taacttacca tctaacccag agtgaactcg ctgaaaaaac aaacttaagt tactcatcta
     3061 ttgtttctta tgaaaacggc ttacgtgatc caaacgcgcg tgcgatggct gcattagaaa
     3121 agttttttgg tgttactggc gagtatttgc ttggcttaac cgataccaat gtctcaaaag
     3181 ctcgtttttt tcctaaagcg aataaaaaat cacccgtgct nnnnnnnnnn cggtgtacat
     3241 tcaaccagct ggaggacaaa tttgttgaat ttcctaatga aattcatctt aaattttcag
     3301 accaggcagg ttgtgcaggc aatggaatct atcttggccc agaatctttt acagagtatc
     3361 ctgttgtaga caacgagctt actcggcgtg ctgcttttgc agttcctgtt tcgggggata
     3421 gtatggaacc taagtttcat aatggtgata ttgtgctggt aaacttcgag cctgtagaac
     3481 cgggagatat tgcacttatc accatggatg gctatgggta tataaaaaag attggcgatc
     3541 aaaaactact ccctttaaac ccgaaatacg atcctattcc aatggatgaa agcgtccgtg
     3601 tgaatgggaa agtaattggc gtatttgacc catcttggta tatctaaatg tgacaatagt
     3661 gcatatatta atcggcaatt tacacttatg cagtatagta tgaaacgcct acaaaaccgc
     3721 tgttttcctt aatcggcaac aaaaacgtta atcggcaaat atttgccgat taaaaagtag
     3781 aagaagccgc ttcggagagg gaaatccccc ttctctaaag cggcttcttt cgtcttttta
     3841 aaacttgtgt ttaagattct ttaaatatca tttaaattcg cagttctatg cggtttttgg
     3901 cgctttttta gtgctgctgt taaagcgtgc ttaaaggtgg tttaaatgcc cgtttaagct
     3961 cccttggcgt tgttcccccg aaaatgaaaa agggccgaaa tcggcccaaa aatttttcaa
     4021 tgatagtttt caattccttg caggatcgtg cgtttttcag ccactgttat gcatcagaaa
     4081 accgctttgt gaggccgttt tgcgggttct tgcgggttct tgcactcaaa actccacgcc
     4141 gttccacatt ttctcattct tcatgaaaac tcacactggc ttaactgtat atgttgtatt
     4201 cccctttcga tttttaccaa cgcttcacgg gtcatgtcta tgtcgagtag ttgtaaatca
     4261 cggacaagag cagtttgccc tat
//
LOCUS       DAWBSB010000190         4272 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_3545_length_4272_cov_2.989803, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000190 DAWBSB010000000
VERSION     DAWBSB010000190.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 4272)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4272)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4272
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3545_length_4272_cov_2.989803"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          784 a         1391 c         1208 g          889 t
ORIGIN      
        1 gcgtaatggc ggtagcccag catgttctgg ccgcggaaca gcatctgtag cttggtgttc
       61 ggcatttcct tgcgcaggat gcgcaggcgg tcccacggat cctcgtccag gaaacggatg
      121 caggagtcaa acgtcgcgcc gccccagcac tctacggagt aatagcccac ctcatccatc
      181 gcaggcagga tgggacgcat ctcgtccatc gtcatgcgcg tagcgatcag agactgatgc
      241 gcgtcgcgca gggcgacctc ggtaatctta atcggttttt ttgccaaaat cgttcacctc
      301 tctgttcagt tcagggaaac gagcagatcg cccacgttca caacgtcgcc cttgcggcag
      361 ttcacggaag caaccgtgcc gtcctgcggc gcaacgatct cgttctccat cttcatggct
      421 tccaggatgc acagcacatc gccggccttg acggaagcgc ccacagcggc cttcacgtcc
      481 aaaatgttgc cgggcatcgg agccgtaacg gtgacagcgc cggtgctgcc tgcagcggca
      541 ggcgcagcgg gcgcagcagc gggggcggcg gcaggcgcag gagcggcagc cggggccggg
      601 gcagctgcgg gagccggagc tgcggcaaca ggcgcggcag cggcgccacc ggcggtctct
      661 tccacagtaa cgttataagc ggtgccgttt acggtgatat tgaaagtttt catgggatat
      721 cctcctgttt attatcttta ctatttcaat tacagagaca tgttgccgtg tttcttcggc
      781 agacgctggg tgcgtttggt ggaaagcata tccagagcgg atacaacggc ggcgcgcacg
      841 cctgcggcgg gcactgccat atcagccagg ccggcttcca cggcagcggc ggcgctgcac
      901 acatcggcag cataccgggc ggccagcttc gcggtatcgg ccgcgatatt ggtgccattc
      961 tccagctcat ccttatacag cacggtaacg gctgccttcg ggtccagcgg ggccacggtg
     1021 cagccctcga cagcgatcgt caggtccgcg tttgccagcg cggtatacgc aacgcccacg
     1081 gccttgcccg tgatgacagc caccttcgcg gtggtagcgt cggcgtaagt acctgccaga
     1141 cgcgcagctt cgcgcaggcc gcccgccata tcatcggaag cgctctttac aaagccgtcg
     1201 gtatttacaa gcgtgacaac cggaatgctg aacgcatcgc acagacgtac aaaacgcgcg
     1261 gccttggaca cgcaattgcg gcacagcaca tcctcggcgc ccttggtggc gacaattccc
     1321 accacattgc ccgccaccgt cgccagcgcg gtgtaaacgg ttttgccata gcccgcatac
     1381 agctcgactg cgctgtttgc gtccgcgatg ctggctgcgg cttccaccgg gcagtatttc
     1441 gccaggttca gcgtgcccgc aggagcggac ggctcaaaca cggcggggcc ggccagattg
     1501 ttggcaggca gcaggcccac cagttcggct gccttggcag cggcctcctc ggcgctgtcc
     1561 acgacaatgg ccgcaacgcc cgccttggcg gcaaactcgg cgctgcccgc gccctccagc
     1621 ttatcgcctg cggcggcaga ggtaaacgga gccgtcaaaa acagctcgcc ctccttgctt
     1681 acaatgcaca catcggcagc ggcagcggcc agagcggagc ccgcaccgca cacgccggtg
     1741 acaacggcga tctgaggcac aacgccggag acctttgcca cggcggcggc aacagccgcg
     1801 ctgccggaaa gcgccgcaag gccctcttcc agcttcgcgc ccacggagtt atagaacgtt
     1861 acgacggggt tgcccgtttt cgcggccata tccagaactt tgacgacctt ctcggtgtct
     1921 ttttcggcca cggccgcgcc tgtctggcac acggcgtaca cgggggtgcc gttcgcgctg
     1981 ccaaacgcag cggtgacagc gccgtccgcg taaagcgcac tgtaagcgcc ttcgtcgaag
     2041 aatttggcaa gcacatcctt gccggttgtt gcgatagcca ttaacagccc tcctgcattt
     2101 taaaaaaatt ccgaaattaa tatcattata ctattttgac aaatcaataa caagtgtttc
     2161 tgttatgatt tccgtaaaat tattcgcaaa tatggaaaat actgctgttg ggaacatcgc
     2221 cctgcgcttc cgggaagaaa acgttcttcc attcggcaag cttttcgtcc tccccgttta
     2281 cccggaagga cgtggccccg gcgttttcgc ctgcggccac acaggcgcgc accgcgccgt
     2341 ccacaaacat tttttcctcc acgccgcact ccagcacctg cgtcaccgcg ccgtccacac
     2401 ggtacagcat gtggcccaga tatttgggac cgtccattac gacatatccc tccacttctc
     2461 cctcgaagcg gccgttgttc aaaagatcga tcacatcata ttcccggatg gggcgcattg
     2521 cgatcatcct ctgttccctc cccgtttttt tcttgccgcc acagcgccct tgggcgcgcg
     2581 cgggccgtga ggcccagccg cggtgcggcg cgcggcgttg gccgccgtat ttttcgcaat
     2641 ggtggccgtg gcctttccgc gggaggcggc cgtgcgcagc gccgtcacct cctggcttgt
     2701 cagctcccgg tactggcctg gctgcagcat gcccagcttt accgcgccca gtgccgaacg
     2761 gcgcaaccgg atgacatcca ggcccacggc ttcgcacata cggcggatct ggcggttttt
     2821 tccctcacgg atggtcattt ccagcactgt ccgctccggt tcgtccgtca ccacatggat
     2881 ggcagcgggc agcgttttgc ttccgtcgtc cagcacaacg ccgtccgtca gcgcaatgat
     2941 ctgctcctcg gttgcgtggg ggcgcaccgt cacgcggtac agcttggaca ctccgtggct
     3001 ggggtgcgtg agcagattgg caaaatcacc gtcattcgtc agcagcagca gcccctcgct
     3061 gtccttgtcc agacgcccca ccgggtacac ccgaacgggc gcgtccgcga tcaggtccat
     3121 taccgttttg cgcccacgct catcgttcgt ggtggtgatg tagccccggg gcttatacat
     3181 catataataa tatttctccg acttcttcgg cacatagacc ttctgtccgt ctaccgtgag
     3241 caggtctttc agcgtgtcca ttttatcgcc cactgtgacg ggcctgccgt tcagcttcac
     3301 gcggccctcg cggatgagct gttctgcggc ccggcgtgag catacgccct gctcggccag
     3361 cactttctgg attcttacgt cgcgcatggt ctctcctttt ctttcccctg tctgatcatt
     3421 ttcccgatgc agcccggcca caaaaaacgg cggagccagg gcgcgcgcgg gccggacagc
     3481 atggcgcgtc cggccgcacg gcacgcccgc gccgccgcgt tattccgccg ccggggccgc
     3541 aggccgggct tcgccctgcg cctgctgctc cttctgtttg ttgttgaaca gcgccgtgat
     3601 cttatcaacc atgtccggca gcataccaat ggcgcggtct acactgttgg aggtatcggc
     3661 aagctgcagc atgcgcacgc taccgtcttt taccaccagg aaagccacgg gctggatgga
     3721 aacacccgca ccgccgccgc cgccgaacag gtccttcgtc gtcttgctgg gaaaatccgc
     3781 gccgccggaa gcgaacccga acgtcacctt tgaaatgggc agcaccagtg tgccgtccgc
     3841 catattgatg ggatttccga tgatggtatt ggaatccacc atgctcttta ttttttccag
     3901 ggtcacatcc atcaggccct caatgggatg ctggctcatg ctgaatacct ccgtttgttt
     3961 ttgatggctc cgcaatgata tcaattggtt cggcgcacag cagccgcctg ccgcagcctc
     4021 taaagcacat gttcattctt cagctgcaca agcgcgtaaa gcgccgctgt tatcattata
     4081 atcggtgatg cgcctatttt acaagagaaa tatggcccgg ccgtgatctc tccggtaaaa
     4141 tcagccaaaa tgcgcacctg cctgaagctc aggttgaaaa aattttgcag cgcggcggca
     4201 gctccgccca cccatgcctg cgctctgcca taggcgacag ccgtctgcgc cgcatcgtcc
     4261 ctgtgtatgg gc
//
LOCUS       DAWBSB010000191         4223 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_3593_length_4223_cov_3.129559, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000191 DAWBSB010000000
VERSION     DAWBSB010000191.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 4223)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4223)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4223
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3593_length_4223_cov_3.129559"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          853 a         1066 c         1466 g          838 t
ORIGIN      
        1 tcggtacgct gggttttcgc ggcggcgtga agctggtgga taaaaagccg atgcctgcct
       61 atatgctgta tgcaggcgga tgcgatgcgc agggggcgga acagttcggc caggagctgg
      121 ggatgatgtt ggaacaggat atcccggctt ttcttgcgga gctggggcgt gcggtgcgcg
      181 cgtccgacgg aacttttgcg cagtggtatc cggcgcatga ggcggaattt aaagagattg
      241 ctgagagata tattgccgac tgaattgaaa acagtctgtc ctgcgtccgt tttgcgggcg
      301 cgggactttt taaaatgttt ggcagcgcgc aaaaaggcgg agcggggtaa tgcggcgtca
      361 aaaagtttac cggaagcata ccggaagatt gacaattttg aacaggaaag acgttttggc
      421 ttgaaagcgg aaaggatttc ccctataata agtgataacc ccatggcggc gtttggcagg
      481 cttctgtcat accgcgagcc ccgcgcggcg atgtgtagag cggcgtttcc gtcatgcggc
      541 ataccacagg gcgggcagcc cacggcttgg gagtattcag tgaaaagaca atggcgcggc
      601 ggaatggccg cttttttgac ttttatcatg ctgatatcag gatgcagcgc ggcccctgcg
      661 gggagcggcg ctgaaagccg taccgtggcg ccggcgaaca gcacacatta cgagccgccg
      721 gcatttcgga acgccgagtt tcacccggac gccgctgtaa gcggcagcgg ggcggaaata
      781 gacctttcgg cggtgtcgca ggggtatgtc gccgtgcggg cgcaaagcga caaacgcctg
      841 aaatgccagg tcgtgtatgg agacgtaaaa tacaattacg atctgcccgg agacggaacg
      901 ccggtcgttt atccgctgca aagcggcaac gggcaataca atgtccgcgt gatgcagaat
      961 accacagaga acaagtacat ggaattgttt tccgcacagg cggacgttgt gctggacagt
     1021 gaattcgagc cgttcctgcg gcccagtcag cgggtagctt acagtgtgga ctccgcatgt
     1081 gtcttgctgg cgtcggacat aagcgcacag gcagcggatg cggcggaggt ggtgagcagt
     1141 gtgtacgaat atatccaagg caatgtggac tatgattacg acaaagccca aaacatcgta
     1201 gacaacaacg ttaccggata tctgccggac ccggatgaga cgctctccac aaagaagggc
     1261 atctgctacg attatgcggc gctcgccgcg gcaatgctgc gcagccaggg tatcccgaca
     1321 aagcttatca caggatatgt ttccagcggc ggaagcgagc tgtaccatgc gtggaacatg
     1381 atttggctgg aagagagcgg ctggatcacc gtggagatca gggcgcccag gcatgaatgg
     1441 cagcgcatcg acctgacgtt tgcggctgcc ggccagagcg cgcttgtggg cgacggtaag
     1501 ggctatgcgg ataaagaggt ctattgagga ggaaaggtca tggcggcaaa aatacttcgc
     1561 tcgctggcgg tgagcgcctt ttgcgaagat atggcgatga tgctggcagc gggcattcag
     1621 gtagacgacg cgctctccct gatgcgcgga gacagcgggg acggcccgct gtacgatgcg
     1681 gcttccgccg tgcttgagcg tgtccaaatg ggggagcctt tggccgaggc ggtggagcaa
     1741 tgcggatatt ttcccgcgta tgcggcgcag cttgtcgcgg cgggcgaggc ggcgggacgc
     1801 accgagagcg tgctgaaaag cctcgcggta tattacgaaa cacaggataa gctggaaaaa
     1861 aggctgaaaa gtgctgtgat ctatccggca gttttgctgt tcttgatggc cggagtgctg
     1921 gccgttctgg tcgcgcgtgt gcttccggta ttcaccggtg tttatacggg gctggcggga
     1981 agcgtagcgg cttcttctta tgcgtacatc acggctgcaa atatcatcgg ctgggcatct
     2041 ctgtgcctgg tcatcgtgct gacggcgctt ttgcttaccg gtgcggctgc ggcgcgcacg
     2101 cagcgtggga acggcctgtt ccgcacgttg tttgaaaaac tgccctttac ttctgctgct
     2161 gcccggcggc tggcggaggc gcaatttact acggcgctgg ccacgtttac ggccagcggg
     2221 atggacaccg acacagcaat ggaacgcgcg tcggacatgg tgcggcaccg cgcgctgcgc
     2281 gcgcagctgg aaagctgccg ccggcagatg cttgaggggc gcagcctggc gcaggccgtt
     2341 tacgataacc gcgtgttcga accgctgtac gcgcgcatgc tgatgagcgg agcacgcagc
     2401 ggcaatctgg accaggtgct ggcgcgcctt gcgcaggtgt tcagtgagga tgcaaacatg
     2461 cggatggggc gcatcatcga cagcgtggaa cctgtgcttg caggatttct caccgtttcg
     2521 gtggggatta ctcttctggc cgtgatgctg ccgttgatcg gcattctcac ttccatcgga
     2581 taagggggcg cggggatgta tcaggaaaaa cggccgcggc gcggcgcaaa acggctgggc
     2641 gcagccatag cggcagtatg tacactgctg ctgttgctgg gtgtatgggc tgtttggaac
     2701 ggcgcacagg cggcaatgag ggaacaggcg gcgcagagcc tccgcacggc ggtgctggat
     2761 gcggcggtgc agtgctacgc tcttgagggg gcatacccgc agagcctttc ctatctggaa
     2821 gaaaactatg gcgtgcaggt caaccacagc cggtttatcg tcagctatga ggcgtatgca
     2881 tcgaaccagc tgccggccgt cgccgtgctg gaaaagcaga cggcatgaca gtcttaggcg
     2941 cgggataaga caaaatgaag ggaggcggcg gagtgaaaaa acacggagcg ttttggatgc
     3001 atctgtatcc gctgcttctg tttgccatca tcgtgctgtc gctgctgttc cttgtgatga
     3061 cggggaccgg gctttacagt gcggtcgtgg acagccagac gcggaacaga gaggcgcgcg
     3121 cggcgctgtc atatcttgcc gcgcgggtgc atgcggcgga cgcggcggga gctgtttccg
     3181 ttggggaagg ccccgagggc gcggcgctgc tgctgcggga gaacgcagac ggcgaaactt
     3241 acgaaacccg tatttattta tatgaaggcg cgctgcggga ggaatatggg acggcagatg
     3301 ctgcgctgga cccgcaggac tctgaacgca tcgtggatgc aaaatccttt tccgtcgagc
     3361 aggttcgacc gggtctgctg gtagtgtcaa ccgaatacgg aagcgcagat atcgcgctgc
     3421 gcagcggaag gggggctggc ccggatgcgt agatctcggg gcggcgcagc tttttatgtg
     3481 gaaacgctgc ttttggtact ctttctgctt gccagcctga ccgtgctggt gcagatactg
     3541 ggtgctgcaa agcgtacctc acgggaggcc agggagcttt ccacagctgt aagcatcgcc
     3601 caaaatgcgg cggagttgtt tgcggcaagc ggctcacagg aagatttcgc agccctgttg
     3661 ggtgctgaga aaacggcgcg cggcacactg cgcgccgcat acgacgtcca gggcggttgg
     3721 acagaggatg aaacacaggg ggcttacgtc ctggaagcag tgctggacga aacgccgcgg
     3781 caggcgggtg agatgcgcac ggcgcatttt gttgtaactg cggcggacgg cgatacggtt
     3841 ttgtatgagc tggatacgca gaaatacatc ggcgggtaac gccgcgggca agggggagac
     3901 gagatggaaa gcaggccggt gcgtctggga tggctgtcgc tgctgctgac ggtgatcatc
     3961 ctgtgtctgg caacgctctc tgtgctgagc ttttccaccg cccgggccga ccttgcactc
     4021 gcgcagaagc aggcgcgtca ggcccaaagc gtttatgcgc tggaatgcgc gggacaacaa
     4081 tggctggcag agttggacgc agcgctggcg caggccgggg atacggcgga gctggatgcg
     4141 ctgctgccgc atggcactgc ccgggaaggc cccggtgtga gcgccgtact ggagggcgcg
     4201 gacgggcgtt cgctttctgt ttt
//
LOCUS       DAWBSB010000192         4218 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_3600_length_4218_cov_3.118184, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000192 DAWBSB010000000
VERSION     DAWBSB010000192.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 4218)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4218)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4218
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3600_length_4218_cov_3.118184"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          840 a         1187 c         1359 g          832 t
ORIGIN      
        1 ctttgtaata tcatcgactc gaacacccat gcaattcccc attttcatca tttctaaaat
       61 caggtgttgc cttgtttcat caccgatggc cgtcaatatc tttgccgagg cggagaagtt
      121 ttctgccaaa gtgaaaattt tttcatttgg ttccatgcac atctcccttt cgttcaaacg
      181 cttaaactgt aaacagtata gcatagaata ttggaaaacg ttcattggta aagaaaatcg
      241 agatgaaaga aaagtttttg gaaaatttta ttccgagctt tgggaaagcc tgccgcgcgg
      301 cgttcatgcg cgggataagt tcagcaggag aaaaaagcag gacagcatag caaaggctat
      361 gctgtcctgc aagattggaa tttttatggg gcggcttttt gatatgcacg cgttacgcgt
      421 tgacagcgtt gtccatcagg tagttgttgg caagctcgct caacacgacc atggacacat
      481 acgggcgtcc gaaatgctct tcataggtgg aatagtcttc ggtgcccatg ttgtcggaaa
      541 aatatttggc gagcgccgcg tcgtcggctt ttagctttgc gtcctcggca atggcctgga
      601 tgatgagaag ctgagtggcg gaagacttga tatcttccgc gtattgctcc agaaacgctt
      661 cctcgctggc tacacccatc gcctgcagga acgtggcgga atcgatcccg tacatggaag
      721 cctgactttt caactggttc agcatggcgc cgctctggaa atccagcaca gcctgcggca
      781 cttcgcttac ggtggagttc tccatcatat agttccagat aaattgaaag atctggcttt
      841 ggcgcagatc ctcgcggatg gaatcacgca cgtcatcggc gctttcccag ccatagctgg
      901 ctgtcaggtt ctcttttacg aaatcgtcgc tgaactccgg cgtaacgtcc tcgccctcga
      961 tataattgat ggtcacttca aacaccgcgt ccttgccggc aagatccggg ttgttttcat
     1021 acgggtcggg gaaggtgacc tccacgtcga aggtctcgcc gggcgtatgg ccgatgatct
     1081 gcgtgagaaa atcgtcgatg aactgcgcgc tgccggcctg tacgctctgg ctgctggctg
     1141 tgccgccgtc aaatttctcg ccgtcgatgc tgccggaata gtcgatgttc acctggtctc
     1201 cgtcctcgat ggcgcggtcg gtgatctgct cgggcttggc gtactgggcc atgatgctgt
     1261 cgatctgcgc ctgcacgtcc tcatcgctca cgtcggcggt gccggcggcc aggggaatgg
     1321 cggcgtaatc ggcgggcagc gttacgtaat ccagcgcggt gacgccgtca atgtggccgt
     1381 tttcagccag gcccttgctg taatcgatgc tctccgcgct ggcggatgtg ctggaagacg
     1441 cgctggcgga agcagacgcg ctggcggaac cggatgcgct tgtgctgccg gaagagctgg
     1501 atgcggaatt gcagccggca agcaaaccgg cgcagagcgc ggcagccagg gcggcgctga
     1561 taaatttcgt tgtctttttc atgggggcct cctaaatcat tctaaaacgc ggcaatgccg
     1621 caatgtcctt attatacacg aattctgtgt acaaggtatg gatttttcaa aaaaaattgc
     1681 ggatttgcgg cttttccgca tggggagcgc gatttttacg gcatgaaacg gcccggcccg
     1741 tagccaagac ggcggaaaac ctgtataata taaatcaaac ctgcatggaa caataccgaa
     1801 agggcggcgg aaatatggat tattacaaag cggcgctgga gctgcacgaa cagcacaagg
     1861 gcaagatcga ggtgacggcc aaggtgcccg taaagacccg ggacgacctg tccgcggcat
     1921 ataccccggg cgtggccgag ccctgccggg agatcgccaa agacccggcg gcggcctata
     1981 cctatacagc caagggaaac ctggtggctg ttgtgaccaa cggcacggcg gtgctggggc
     2041 tgggcgatat cggcgcggcg gcggccatgc ccgtcatgga gggcaaagcc atcctgttta
     2101 aggagttcgg cggtgtggat gcgttcccca tctgcctgga tacaaaagat ccggagcagg
     2161 tcatcgaggc cgtgcgcctg ctggctccca ccttcggcgg cgtgaatctg gaggatatct
     2221 cctcgcccaa ttgcttctac attgaggaaa cgttggaaaa ggagctggac attcccgtgt
     2281 tccacgacga ccagcacggc acggccatcg tggtgacggc ggcgctgctg ggcgcgctgc
     2341 gtgtggtgaa aaaggagatc ggcgccatcc gcgtggtggt gaacggcccg ggcgcggcgg
     2401 gcacggccat catcaaaatg ctgctggcgg cgggcgtgcg ggacgtgatc gcctgcgacg
     2461 aaaacggcat tttgtacaaa gagcggggcg tgggcatcgt ggaccacaaa gagatgctgt
     2521 gcggcatcac gaacaaaagc ggcatgcgcg gcacgctggc cgacgcggta aagggcgcgg
     2581 atgtattcat cggcgtttcc gtgggcggcg cgctgaagcc ggagatggcc cgcaccatgg
     2641 cgcgggatgc cattgtattc gccatggcca accccgtgcc ggagatcaca cccgaggagg
     2701 ccagggcggc gggcgtgcgc gtgatgggca cgggccgcag cgattatccc aaccagatca
     2761 acaacgtgct ggcgtttccc ggtgtgttca agggcgcgct ttcggtgcgc gcgcgggaca
     2821 tcaacagcgc catgaaggtg gccgcagccc acgccatcgc ggatcttgtc agcgacgaag
     2881 aacgcaatga ggattacatc atcccgggcg cgttcgacgc gcgtgtagcg caggcggtag
     2941 ctgacgccgt cgcgcgggcc gcgcgggaat cgggggtggc gcggctgtga gggacatccc
     3001 tgcgagcgct gtcaccgccg ccgtaaaggc gctgtgcgtg ccggccaaca accagctccc
     3061 gcccgatgtg gaagccgctg tgcgcggcgc gtatgaaagc gagccgtggc ccattgcaaa
     3121 gcagacgctg ggcacgctgt gccaaaacct ggacgcggcc cgggccacgg gcctgcccat
     3181 ctgccaggat acgggaatgg cctgtgtatt tgccgaaatc ggccaggatg tgcatatcac
     3241 cggcggttcc tttgaagaag cggtggacga aggtgtgcgc cagggctata ccgagggata
     3301 tctgcgcaaa tccgtggtgg cggaccccat ccgccggggc aacacggggg acaacacgcc
     3361 cgctgtgctc acggtgcgtc ttgtgcccgg ggacaagctg cggctgacgc tggcacccaa
     3421 ggggttcggc agcgaaaaca tgagccgtct tgccatgctg cgtcccagcg atggcgagca
     3481 gggcgtgaaa gattttgttc tggacgctgt gcgccgggcc gggccgaacc cctgtccgcc
     3541 cgttgtagtg ggcgtgggca tcggcggcac gttcgaccat gtggcccttc ttgcaaagcg
     3601 ggctctgctg cgcccgctgg gcagccgcca tccggacccg tactatgcgg ccatggaaca
     3661 ggagcttttg gaaaagatca acgaactggg cacggggccg cagggcttcg gcgggcggac
     3721 cacggcgctt gggctgcaga tcgaaacatt ccccacgcac atcgcgggcc tgcctgtggc
     3781 ggtgaacatc aactgccacg taacccgtca cgcggaggtg gtgctgtaat ggaatataag
     3841 ctgacggccc cgctttccaa agcggatgcg gcaaagctgc gcgcggggga cacggtgctg
     3901 ctttcgggaa ccatttacac ggcccgggac gccgctcaca agcgcctgtg cgcgctggcg
     3961 gcagagggca agcctctgcc ctttgccatt gaggacgcgg tcatttatta cgccggcccc
     4021 acgcccgccc gcccgggcgg ggtgatcggt tccgtgggcc ccaccaccag ctaccgtatg
     4081 gacgcctacg cgcccacgct gctggacatg ggccagacgg gcatgatcgg caaaggcgcg
     4141 cgggggccgg aggtcgtggc ggccatgcgc cggacgggcg ccgtatactt tgcggctatc
     4201 ggcggcgcgg gtgcggcc
//
LOCUS       DAWBSB010000193         4196 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_3624_length_4196_cov_2.904854, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000193 DAWBSB010000000
VERSION     DAWBSB010000193.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 4196)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4196)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4196
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3624_length_4196_cov_2.904854"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1031..1040
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3638..3647
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          937 a         1003 c         1356 g          880 t
ORIGIN      
        1 ccgtcaaaac aaaggacgac tggctgaagg tcaagcattg gtatgaattt tcggaggagc
       61 gtattgaccg ggaggcgctg ctgcatcaaa aggaactgcg cgataaaggc tatctgacga
      121 tccagtgggt gcccggcggg ttcgacgagc cgaggcagct tatgggcgag gaagaattgt
      181 gtatcgcatg ctacgaggag ccggagttga tcgccgacat gttggaaacc atcggaaaca
      241 cctgtgtaaa agtgatggag cgtgtggctg agatcgtccc catcgactgc ctcagcatcc
      301 atgaggacat ggcgggaaag tccggccctc tgttcggccc ggcgcagatt gaagcgtttt
      361 tgcagccgta ctatcaaaaa atatggaatt gcgcgcgggc ccacggagca aagatattct
      421 cccaggacag tgatggaaac atgaaccctg tgatcgacgc gatcctcgcc tgcggtgtga
      481 cgtgcctgta tccctgcgaa ccggcggcgg gaatggatat gatggagctg cgaaaaaagt
      541 acggcgccag gctggccttt aaaggcggca ttgacaaatt tgtactgcgc ggcgatttcc
      601 aagccatccg cgcggagctg gaatacaaga tgtccggtcc tctgatgggc ggcggcacag
      661 tgtttgccat tgaccaccgt atcccaaacg gagtaccgct tgccaattac cgctattatg
      721 ttgagaccgg gcgcaggttg ctgggtctcc cgcccgcgag ggaagagggc tgggcgcgca
      781 tggccttctg acggatatcc gctaaaagcc gggccggaga tatttccggc ccggcttttc
      841 cgtgcagaaa gaagatacct ttttctgcat gaaggcgtct tttgcataag cttttaaggt
      901 ggtatactgg gcatagcaag gatgcagacc acaaaaaaag gagaaatcgg acgatgacgg
      961 ttgtggaaaa aatcgtcgcg ctgcgtgcgg ctatggaagc ggaaggagca gacggcgtat
     1021 atgtgccgtc nnnnnnnnnn acggaatacc tgccggcccg ctggcgctgc cgtgcgtggc
     1081 tgtcgggctt tacgggttcg gcgggaacgc tgtgtgtgac acgggaagcc gccgcgttgt
     1141 ggacggacgg acgctatttc atccaggcgg agcggcagct ggagggcagc ggaatcacgc
     1201 tgatgcgcat ggcccagccc ggcgtgccca ccgcggagga atggctggcg gacaggatgc
     1261 ccgccggagg aacgctggca ctggacggcg cctgcaccag tgtgtccgtg gtggaatcgc
     1321 tggaaaaagc gctggcggaa aaaagagctg tcctgcgtga ccacgatttt gtttccgcac
     1381 tttggcagga ggggcggccg cccatacctg cgacagaagc atggctgctg gagccgtcgc
     1441 gcgcgggact ttccgccggg gaaaagctgc atatcctgcg ggagaggctt gcccggcaga
     1501 acgcaaacgc catgctggtg tgccggttgg acagtgtggc gtggctattg aacctgcgtg
     1561 cggacgatgt ggagtacaac ccgtttgcgc tcgcttactg cctggtggag ccgaaggggg
     1621 cgcatctgtt cattgacacg agccgcgtgc ccgccggggt acagacagtg ctggaggcgc
     1681 agggagtaac gctgcatccg tatggggata tacgggactt tctggctgcg gcggaaacgc
     1741 cgatggcgct tttgtatgag cctgcgggta cgagctggac cctcctgcgg gcgctgcgcc
     1801 aaaaccgggc cgtgacgctc tcggcgggta aagaccccct tcagctgctc aaagccgtga
     1861 aggatgaaac agagatggcc ggccaaaaag aggcgcaccg cagggacggc gcggcgatgg
     1921 ttcggttcga gatggagctg cgccgccgca tggaaacagg ggaaggctgg acggaatttg
     1981 cagcatccaa ctatttgacg agcctgagaa tggcgcagaa ggactgcctg ggcgcaagct
     2041 ttgaaaccat cgcggcctat ggggcaaacg cggctatgat gcactatgcc cccatgaagg
     2101 atacctgcgc ggagctggag ccgcgcgggt tcctgctggt ggacagcggc gggcaatacc
     2161 gggacggcac cacggacatc acgcgtacct atgcgctggg cccgctgacg gacgaggagc
     2221 gggaggcgta tacgcttgtg ctcaagtgcc acattgccgc cgcgcgcgcg gtgttcaaag
     2281 cgggaagcgc gggcatgcat atcgacattc tggcgcggga aacactctgg aggcgcggcc
     2341 tggattaccg ctgcggtacg ggccacggcg tgggatttgt gggggttatc cacgagggcc
     2401 cgcagggcct ttccgcaaaa aatgacacgc cgtttgaacc gggcatgacc gtcaccgatg
     2461 agccgggcat ctacgaagaa ggcaaactcg gcgtgcgcat tgaaaacgaa ctgctgtgcg
     2521 tgccagtatg tgaaacagaa tatggagcgt tttatggctt tgaacccttc acctactgtc
     2581 ccattgacac gcgccctctg aagctggagc tgatgaccag agaagagctg gaatggctgg
     2641 accgttatca tgcggctgtg cttcgggaac tgggcgcgta tctcaataaa gaggaaggtg
     2701 catggctgga aaaggcgtgc gcgccggtgg cacgatgaat gtgtgtattg ccgttttgct
     2761 gtgttttgcg ctgcttggag cgctggatga aggaagcgga ggcaggctgg gggtcctgtt
     2821 gttttgctgg atgacgtggg gcctgtttgt accggaacgg tccgtaatgc cgcctgacgt
     2881 agtggaggaa gtgctggtaa tcattctgaa gatcaccatt atcgtatgtg gttctctggt
     2941 agctggcagg ctggtactgg caaagtgcag ccggtggctg gagtgggccg catccaggct
     3001 gggcgtcaat gaatatgctg ttttggggct tttgacaagc cttgtctcaa gtgtttccat
     3061 gctgccgctg tatccccgga tggatgccag agggagaatg ataaacgctg cgtttacggt
     3121 atccggggca tttgtattgg gcgggcaaat ggcctttgcg gctggcgttg cagacggacg
     3181 cgccgttgca gcatattttg caacaaagct ggccggcggt ttattggctt tgatattggt
     3241 gttttgcttt gcgaaaaata cggaagcagt atacacaaca gatatatccg taacgcaggg
     3301 tcaaaaataa tacaggagtt gacttttgcg ggtggctgcg ccgagagaaa atacaaaaaa
     3361 tattccgaat attgtttttc ggcaggcggc tgtatcacaa gatttgttga atttcttcaa
     3421 aactcctact gttaaaatca agagcttgct ccacgttttg cccgtacctt gagaaatgcg
     3481 gtatccatac tgtttggtga gtttcattac aggccggcct gtgtgggatt cgtccaaaca
     3541 gtatccgccg gtacccgaac aagcatcgga ggctgctgaa aggagaaggc ggctgcatac
     3601 tcaaacgcat atgcagcggc aggacaagct gcttgagnnn nnnnnnnctg cttgaacggt
     3661 aacaacagtg acacacttga tggtacttcc tgaagtgatg gcgaaaacac cgttgtcatc
     3721 cttacggtca aaagatttgt ccaatgcttt gcgccaaaag ctggagagag gaagcaatcc
     3781 tttctggcat cgaaaatatt ttacagcgtc gctttggcac aaatgcggcg aagtcggata
     3841 ccgctgggga taccacgcgc gcaggaaaga ggctgtcaga agcgtggttt ccatcctgac
     3901 aaaacttaaa aggtcttaaa cacagtcata actttgcaaa atgaaaaaca gccctcggaa
     3961 aattattccc gagggctgtt ttggtgcgcc cggctggaac cgaatcggcg gctttccggg
     4021 tctgagagct gtgacaaagg gttttaaatg gtaataatcg tgctattttg ggttgggata
     4081 cacttaattg taaagggcgg tgaaaagccc ggggagatgg aggccagcag gttccgcgga
     4141 ttttttgtat atcagtagtc agatagtggt cgggagagcg tggactgcga tgatct
//
LOCUS       DAWBSB010000194         4194 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_3626_length_4194_cov_1.758154, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000194 DAWBSB010000000
VERSION     DAWBSB010000194.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 4194)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4194)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4194
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3626_length_4194_cov_1.758154"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1218..1227
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3935..3944
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          920 a         1196 c         1050 g         1008 t
ORIGIN      
        1 gctcacagta ttccacccgc agggtctgtc cgtcgaactc ggcggaaaag gctccggccc
       61 ggcgggctga gagccgtatt ccgcccgtgc cggaacgaaa tccctcgtgc atacacagtt
      121 cttccagacc gctgcgcagt tcgtccggga gcccgcataa atcaattttc acagtgttca
      181 tcggtaaaac tcctttaact tatctttcag cagcccgctg tatagtccgg ccgtgatttc
      241 cgccacagtc cagccgcttt ccaccgccca agggccccag cccacgtcat tgggaagccc
      301 aaacacttcc atcatatcca catcgtatcc gcgcgcccat gcgccgttga tttttgcatt
      361 cctgctgctg atctgggcgc tggcaagaaa cccggcatgg cggaggaaca gagcgtagat
      421 attttcgtct cctgtgacga aataagcctg catcaggccc ataggcaccc agttgttgct
      481 gtacagcaga tctgccacag ggtctccgtt ccggctcagc agtgaacatt cgccaccctc
      541 ctgtctgctg catgcggcgg aatagtcgct gtcccactcg agatatgcgc cggaaggatg
      601 gcgcacggcc tcaaggctgc ggatgacagt gtagagcatt tccaggtccg tctccttccc
      661 cgtggcatag tacagccatg aaacaggcag aagcaggcgg caaagctcct cggtctcgct
      721 ctgctcgcgc actgttttgg gataggccgc cagaagagag cgcatgcccc gctccccgac
      781 ctccagacac cgggcgtccc gttccatttt tccgtacagc aaaagacacg caagataaaa
      841 cgcattgtaa tgcgcgcagg gaaacgcgcc gttctgcgtg tgcagccgtt caaaatccga
      901 ctgcgtcata ttcaaattgt ccgtgcgggc cggccgcagc ccgtccgggc cggtcgtatt
      961 catcaaaaac tccagcgcca tacggcattg cggcagatac tcacgtccct gcccatacag
     1021 cgccttgagc agcgttacca gcatggcccg ggccatatcg tcctgatagc atacttccca
     1081 cgcgacatcc gtccagcgca gcatgcctgc acagcgcccg gtcttgacct gcattacgtc
     1141 gtagacgtaa gcttccagcc ggccggaacg tgcccggctc tctgcgtcac ccgtagccag
     1201 cgcatgaaaa aaataagnnn nnnnnnncgg atgggaaacg cgattttctg atggccgtcc
     1261 ggataaatct cagtgccaag gccctccagc acgccgccgt ttccgccgtc aaggagcgtg
     1321 ccggatgctt caaaccagtg caacgccgcc tgtgcgcacg cgcccagttc actgctccgc
     1381 cccagcgtat aggccggctc ggccggaggc aattctacag gcgtgccgca aagccattcc
     1441 accaatgcag caactacacg ccgccacacg ctgacaggag cgaagcgcgc ccgcacaaaa
     1501 ttacacagac ggaaactgca caccgctgta gtaggtgttt caaaccagac gccgtaagcc
     1561 gtgtgatctt ctttgtcgct ttcggagagc ggttcgcatg cgtgctgcgt caatcctttt
     1621 ttatacacga gcacaggccg cgccccgcgg catttataat aaggagccga ccggaaattg
     1681 cactgctcgt ccagcagcgt cccctccggc atgccggtga atgcgccgtc cgcacacaca
     1741 aggcgcagga accgcgtgga ttcgggcggc atgcagtagt tctgcgcaaa gctgagcgca
     1801 tattcgtaaa acaccctttt gcccgcggcg gaaaacgctt ccaatgcgct gcggctccgg
     1861 gcgggcagta ccagcggctc ctcatgcgtg ccgccaaaaa cgcaggcaca gtcaaatgta
     1921 tccaggctgt gcgggcccag cgcatgcggc gcaaaaacga cagtggacac cccgtcgcac
     1981 cgctgcaaaa gccgggagag atcgctttct ccccgtgtga tgatcgcgac agaaacagac
     2041 atgtcttttc ctccttcaga ccgttttgtc cgtggtttaa ttccattata ggagcagggc
     2101 cgcataatgc aacggaatgg aagaattgct ggccgcgaaa ataatgcgca gcaatttttt
     2161 tactgaaaaa attgtttttc cggcggcaaa aatattgcgt gcctgctgaa aattttgttg
     2221 attgctttcg ccggaggggg aatgtaaact tgattttgga aaacaaagct gcgaaaggtt
     2281 ggtggcaagc cgtgcaaaag acaacggagc aaaaggcagc gggacgtccg gcgcgtctga
     2341 attacaagat gaagacgcaa ttacaactgt atgcactcct cgctcccgtg ctgattttaa
     2401 tttttatttt ttgttatttg cctttatatg gcctgctcat tgcctttcag gagtggtacc
     2461 caggtatgcc gtttctggac tttacgggtg ctaccaagtg ggtaggcctc aagcacttca
     2521 tctcattttt tcaaaatcca tatttttggc gtttgatcaa aaacacactg gtgctcagcg
     2581 cactgaattt gggtgtagca ttctggatcc ccatcgggtt tgcgctgctc atcaatgaac
     2641 tgcgtctggt aaagttcaaa aagtttgctc aaacagcaag ctatctgccc tatttcatct
     2701 ccagcgttgt tgtggcgggc atggcgcttt cattcctcag cagcgacggt attatcaaca
     2761 aactgatcgt actgctgggc ggcgagccgt ttcttctgaa tatgaagcca cagtattttc
     2821 caatggtcta cacgctcatt aatatatggc gaaatttcgg atggggcagt atcctgtatc
     2881 tttcgtcgat ggcgtctatc gacacggcgc tgtacgaggc cgccagcctg gacggagcaa
     2941 atcgctggca caaaatgtgg cacatcacac tgccctccat caagccgacc atcgtcgtca
     3001 tgctcattat gggcgtgggc ggaatgctga acgcggattc ggatatgatt cttctactgt
     3061 acagcccagc tacttatgag tacgctgacg tcatcggcac ctatctgtac cgctatggcc
     3121 tggtgggagg caatttcagc tatggcacag ctgtgggcct cttcatctcg ctcatcaact
     3181 tcggccttat gttcctggcg aacaaagtct cacgcagggt cgcagactat tcgttgtggt
     3241 aatttatttt acaaagaaag ggtgactttg catggctcgt acttctttcc tgaaaaagtg
     3301 gcggggtgtc aacaaccata ttcaggaagg ctcccgcatg gttgacgcgc tgatcctggc
     3361 cgtcatcgtc ctgatctgtg ttctcacgct gtatcccatg tactatgttt tcatcatgtc
     3421 catcagcaaa ccgcaagatg tgatggcgat gaacgtctat ctatggccta ccggttttga
     3481 gctggaggca tataaaaaga tttttaccga cagcaacatg tggcgcgctt atgcgaattc
     3541 catcttatac actgcgtctt ccacaatatt gattcttatc accagcgtgc tgggtgcata
     3601 tcccctttcc gtaccggagc tgcgcgggcg caaattcgtc gtagcgttcg ttctgctgcc
     3661 catgtatttc ggcggcgggc tgattcctac gtttttggtg atgaacagtc tgcatcttta
     3721 caataatatt ttcgcgatca tcatcccggc gtcgttcagc atctggtata tcattcttac
     3781 aagaacctat ttcacgggta ttccgaaaga tatccgggaa tctgcgttta ttgacggcgc
     3841 aagcgatgcg gtgatcctgt tccggatcta tcttcccctt gcaaagccta ttctggcggt
     3901 gattgccatt tactccatcg tctccatgtg gaacnnnnnn nnnncaactg gttttcggca
     3961 caggtgtatc tgccggatca ggcgctgcac ccgcttcagc tgtacctgaa gcgcgttctt
     4021 gtggcgcaaa cggtggacct taccaagctc aactctgccg atatcggcaa cgccatgcgt
     4081 cagatgatct ccgcgataca gatgcaatat tccatgatta tctttacgac gcttcccatt
     4141 atcttcactt acccgttctt ccagaaatat tttatcaaag gcgttatgct cggc
//
LOCUS       DAWBSB010000195         4181 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_3633_length_4181_cov_3.143965, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000195 DAWBSB010000000
VERSION     DAWBSB010000195.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 4181)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4181)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4181
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3633_length_4181_cov_3.143965"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          841 a         1258 c         1229 g          853 t
ORIGIN      
        1 cgtggcctgc actttgctgc gcaaagatgc atggctttgc caccgccagc ttttttcccg
       61 gacgttcctt ttccttcttt gcctttttcg accgtctgaa aaatcatttc ttttcaaccg
      121 ccacccagat ttcgcaggtg tagttggggt cgtccacatc tgccgaggga tacgcctcaa
      181 aatccgttcc gccgcagggc tgatattcac tggccgggaa aaattcgctg catacctttt
      241 ggtaaaggtt ttgaagtgcc tccggcattc gcccggtgca gggaaataca acataagtgt
      301 gggccggaat gtcttctacg gtcaatcccg catccgtgac gggtgctccg ttgtaaggcg
      361 cgccgatggc gtaggggaat tccgcatccg cggcatcccg tccgaaactg gcacccacga
      421 ttcggtcgcc aaagatattg ggttccggga tatagcggca cagcgcctca atggttccgt
      481 cggtggtgca ttcgcgccaa aatggggaga tcaccgcctc cgtcaattcg gcccgactgg
      541 gaatttgctt ttttcggcag atgagccgga aggcttccac tgtttcgatt ctgtaatcca
      601 ttgtgcttcc tccatccaaa gtaagtttca cggaaagagg ggaaaaagct ttgagcgccg
      661 cgccatgctt tgcctgtgag ggaggcacgc cgtgaaagcg ggtaaaggca cggctgaaac
      721 tctccggcgt atcatagcca tatttcaaag ccgcgtcgat tacccgaatg tccgacgatg
      781 caagttcgct gccggccaga gtgagccgcc gcattcgaat atagtctccc agcgtaaagc
      841 cgcacaggat gctgaacacc cgctgaaaat gaaagctgga cgagtaggcc tgcttcgctg
      901 cttcttcata gtcaacggat tccatcaaat gggcttccac gtagtccagc gcccgctgta
      961 tcccggtgat ccaattcatt gcgccatccc tttccgcgtg attccatgat aaaggaatcc
     1021 gcgctcccgt tcctgacatt ctgtgctgtc cgctgtcacg cggaggggga gcggcggaag
     1081 aattttattt cagccagccg cagcgggcca tgtctacgcg gccgtccggc aggaaaacca
     1141 cgccttcttc ctccagcagt gcgcgctgta tgccctcgcc gccaaaggca aggccgccgc
     1201 acaggccgcc gtctttaaat accacgcggt ggcacgggat gccgcccggc tcggggctgc
     1261 ggtgcatcgc gaagccgacg ccccgcgcgc cgcgcggatt cccggcaaga aacgccacct
     1321 gcccgtaaga ggccacgcag ccccgtggga tctgccgtac gacctcataa atgcgttttg
     1381 tgaattcaga cgccatgctc cccgctccct ttctgccccg cgcgcgccac gcgcacctgg
     1441 acctccgtca aatactcgtc cacccgcgcg gtgtcgtggg cgtcgatgtg gtaaagttcc
     1501 atcacaggcc cgtcctgccg caggccctgt gcggccagac ggggccacag cgcggcgcat
     1561 acgtccgcaa gctggctgta cggaccgcgg taaaaaacgc tggcgtaagc gcccgcgggc
     1621 agcgcgccgt ccgcttcccg cctgcggcag cccaggtaga acacgctgca aaaatggttg
     1681 tagatccccc gggcgcgctg cgattcgtcc agcaccgcgc ccatgcactg ggtacccagc
     1741 gcacgaatga cctcctcgtg gcggtgggca agttttttca gtgcgaagtc gatctcgcgc
     1801 tccaggataa cgtcctcctg caattgcaga cagggacgtt ccggcagctc cagcaggcgc
     1861 agcaggcccg gttcctgccc gtcataccgc tccaaacgcg cccggcgcgc atccacggcg
     1921 gcacgcgcgt cccgcagggc ggcaatgcgc gcccggatgg cagcgtcctg ctcgtccagc
     1981 atgtccagtg tggaggccac tgtacgttcc ccgaaataac ggcgcatgtg ctccacgggc
     2041 atgtccagct cccgcagggc gcggattacg ttcaggttac agatatcgtc gatgctgtac
     2101 aggcggtagc ccgcctcgct gcgatgggga tgcagcaggc cctgttcctc ataataacgc
     2161 agcgtgtccg tgcaaaggcc gtacagccgc gccacctcgg tgatcttgta atattccttc
     2221 atccaattcc tcccggcggt gtaaaaggct ttgtaaaaag tataccgtcc atgggcggag
     2281 aatacaagcg aaaaagatgc ttgaccttag gacgatacga gggtttataa tggctttgca
     2341 aacattggaa ggagatttga tacccatgag acccgcaaag gaacccctga cacgctatgc
     2401 tgtgctgctg ctgggagcgg ccatcctcag cttcggcttg ttcaatgtcc acagccagag
     2461 cggcgttacc gagggcggtg tgctgggcac cacgctgctg ctgcatcact ggttcggcat
     2521 ttcgccgggc gttagtgaat ttgtcattga cgtcatctgc tacgcgcttg gctttcgttt
     2581 tttgggcaag gcgtttttca aatatgccat cgcggcgagc gcgagcttcg cctgctttta
     2641 tatgctgtgg gagcgtgtcg gcccggtttt gccagacctt tccggccaac cgctgattgc
     2701 cgcgctgctg ggcggagtgt tcgtgggcgt tggcgtgggc cttgttgtac gtgcgggcgg
     2761 cgcgtccggc ggcgacgatg tgctggcgct gctgctgaac aagtttacga agctgacgct
     2821 ctccaaatcc tattttatga cagacgccat tgtgctggcg ctgtcgctga cgtacattcc
     2881 ggcagggcgc atcgtattct cattcattac ggtgacgctg tcctcgttcc tcatcgagcg
     2941 catccagcgt atcggcgcgc cgtgcgcggc ggcggagcag ggagcatagg gcgaaaaaca
     3001 aaaaacgggc gcggcctttt gaccgcgtcc atttttggct gaggacaatg gtgcgtcaac
     3061 catgccgacg ccgctccagt gcgcgcaaat gggatttctg ctctgcattg acctgatgag
     3121 attcacaaat tttccggatg gtcctgcggt aagtaaattc gtccagccgc gcggcttgca
     3181 gccagggcag cgtctcgtcg ggaaatgcgg catagcacat ggaggcagcc catgcaacag
     3241 ccatttttac atagtattcg tcgctgtgta cttcctccag cagagcaagc acgcggggca
     3301 gatgctgcgc gtcgatgtaa taaaacagca gcatcaccac ggcgaaacgc acggcaaagg
     3361 ggcgggggtc ctcaaaataa ggctgcaaaa attcccacac cggctccgga tgcgtgcggg
     3421 ccagcttcag tccgctgcaa aagctgtcgc acaccgacca attatctatt ttcggcacga
     3481 aagcagcggt gcgggccaga acctcctgcg gcggcgcgca gatagcacca atgaccattc
     3541 cctgcagcat gacttcttca aacgagccgt ctcctgcggt atccagatag gcggcccaat
     3601 cccccttggc aatgcggcgc gcaaggcggc gcagcagggg aaggcgcacg cccagcacgt
     3661 tttccgtgcc gggcaaaagc ctgcgggaaa aaacggcgta ttccggttcg gccaggcgga
     3721 caagttcagc ctttatgtcg atctccacac aaatcacccc tgtccattct accaaaatcc
     3781 ggcgggatat gctacaatat gtgcagagac gcgccacacg ggcgcgtgac agaagcacac
     3841 agcgccgggc cgcgcagggc gacggcggaa aggacgaggg aatgctgaag atcgggagcc
     3901 atatttcttc ctccaagggc tatgccgcca tgggacggca ggcgcgcaaa ttgggagcga
     3961 acacatttgc atttttcacg cgcaacccac ggggcggaag cgtaaaagcg ctggatgagg
     4021 ccgatgtggc ggaatttttg gacaaagcgg cggaaaacgg cgtggaaggc cccatcgtgg
     4081 cccatgcgcc ttatacgatg aacgcctgtg cggcggaccc gggcctgcgg gaatttgcgc
     4141 agaacatgat gcgggacgat cttgcgcgcc tggagcatac g
//
LOCUS       DAWBSB010000196         4159 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_3652_length_4159_cov_2.846735, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000196 DAWBSB010000000
VERSION     DAWBSB010000196.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 4159)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4159)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4159
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3652_length_4159_cov_2.846735"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          855 a         1304 c         1018 g          982 t
ORIGIN      
        1 tcaaatcagc cagtcgattt ttatttcctg cgcacctgtt ttatcatctc tttctcctat
       61 ctgaacatct tgaataaaaa gagccgccag ttcccttgtc agagcggatg cttccgtaat
      121 ctcctgtaag taagtctcca gtttcgtcct gcctcccggc gctttttgtt cctctttttc
      181 ctcaagagcc aatgcttcga gcctgtgtat caattctttt ttgtccgcaa ggtaaacccg
      241 gttcagttcc agaaactgtt cttcttccag aatgccgcgg gatttgtcca aatacaggtc
      301 gtgcaatgcc ttgatccgct gctcaagctc catcttcagg cgcaattgtt cctttgtacg
      361 gcgtttccgg tcgctctgtg caagatcttc catagcgcat acatccgcct gctgcggatc
      421 gaaatagcga tttatatgac attgcagccg gcgcagcacc tcctcctcca accagtccag
      481 gcggatgtta tgatttgtgc acagaggctt gtgcgtcgca tacagcttgc acctgagata
      541 agaatatttg ccggcggccc ggccgttgct gtagcgctgc atcgcgctgc cgcactgcat
      601 acaatggact ttccccgcca gtggatgcac ctctcccagc cccgtggagc gtgtgcgcgc
      661 atgcagcatt tcctgtacgc gctcgaacgt tctgcggctg atgaccggct catgggaatc
      721 cggcacaatg atccaatcgc gctgcggtac agaagccatc tttttggatt tatagctgat
      781 tttttttgtg cgcccctgta caaggtctcc cgtatacatc tgatcccgca ggatacggct
      841 tactgtcgtg cggttccaca gcccatggtc gttgtgcgct gcgccgttac ggtacggata
      901 gcccatcgcc tgcttgtgcc tcgtcgggtt cggcacgccg tcccggttca gaatggccgc
      961 gatgctctgt gtgccatgcc ccgccagata cagcgcgtag atgcgccgca ccacggcggc
     1021 cgcctgcggc tccacgatca gcttattttt atccgccggg tctttccggt agccgtaaag
     1081 cgggaaccct ccgatgtatt gtcccgcccg gcgcttgtag tcgaacacag tacggatatt
     1141 ttccgataag tcttccagat accactcgtt gacaaggccg ttgatctgcc gcgctttttt
     1201 gttgcccttg atgtccgtgt ccacgttgtc gaccgtagcg acaaaacgaa tgccccattc
     1261 cgcgaataag ccgtggatgt atttttcgac caattccatg tcgcgcgtaa agcgcgactg
     1321 tgttttcacc agcacaatgt cgaaccgccg cgcctctgca tccgcgataa gctgtcgaaa
     1381 ggccgggcgt tcccggtcaa tgccggagta atcttcatca caataaatat tgtaaatgtc
     1441 ccagcccttg tccagcgcgt acttgatgag catggatttc tggttttgga tgctctcgga
     1501 ctccgattgc ttctcctcat cctcccggct cagcctgcaa taaatcgccg ccgtcatggc
     1561 actacctcct gcacagtgtt ctgccgcaac ggcaatactg ccacatcgtt ttcaaaacag
     1621 ccgtcacaca caggaagaat tttctgtacg ggattgtaag tattgctcca ttttctgctg
     1681 aacggatgtc ttcctttcag cctctgtttt cgcctgaaag gattctgtta caataaatat
     1741 tggccgtttc atatagatcg ccccggtaat acttatgcat cagcagcccg tccctatcgc
     1801 tcggcaaaca ggacattctc cggcaaggag ccgcactgcc tgtataccgc cactccgttt
     1861 tcgcaaataa gaacatcttg catttggctt ttagcgcgga gtccatcttg aatgcgcata
     1921 aattccattt ccacaaaaaa gctccccgga acagtcaatt gggctgtttc ggagagcttt
     1981 ttcttattga ttcgtatctt tttgatttgc agatttgcgt tacatcctgc aggcgctgca
     2041 cccctgaggg catgcaggag aggcatcgcc ctcttcattt ttgccgaaca cctgttcgta
     2101 cagccgcttg cctgccggcg tcgccgcgag gcctccttct gctgtttcct tcaatgccgt
     2161 gggcatcgcg tcgcctacgc gcttcatcgc gaggatcgct tcatcggcgg ggatagggct
     2221 gcgaatgcca gccagcgcga gctgcgccgc gacaagagcg cccgccacgc ctgccgcatt
     2281 gcggtggatg cagggaattt ccacaagacc ggccacaggg tcacacacaa ggcccaatac
     2341 attgcccaga gagatggaca ccgcgtcacc cagcatatcc ggcgtgccgc cgcacaattc
     2401 cacaagagcc gccgcagcca tggctgcggc actgccgcac tcggcctgac agccgccctc
     2461 cgcgcccgca atgcacgcat tgtttgcaat caccatacca accgctgacg ccgtgaacag
     2521 tgacatcacg caagcgcgct ccggcagctt acgctctgcc tgcaacgtga gcacggcggc
     2581 cggcagaatt ccgcaggagc ctgccgtggg cgccgcaacg atacggccca ttgccgcgtt
     2641 ccattcgctg actgcaagcg cgcgggtgag cgcgccggag aacaccccgc cgcacagcac
     2701 gttcccttcc cgctctgcac gtgccatacg gtaagcgtct ccgcccgaaa ggccgctggc
     2761 cgaacgtacg ccgggcgctt tcccctcctc cacggatgcc gccattaccg cgtagctttt
     2821 gcgcatgatt tcatacaatt cgtccgcgct ctgttccatt tgtaccgcct gctgctccag
     2881 caccagctca gagagcttcc tcccgctttg tttcgctgct tcgatcaatg cggcgattga
     2941 atcatatctt aaatccattc tcattctccc ccgttacatc ggtgcaagca tcgtggacga
     3001 caacacattc ggaagttttt ccacacagga attcacatca ggctccagcg cgccgtccac
     3061 ctcgatggtc atgaccgctg ttcctccttt ttgtgcgcgg gaaaggcgga aattacagat
     3121 attgacgccg cgccggctca tttcctccgt aacggcggca atcgttcctg gcgcatcacg
     3181 gtgcagtacg atcagcgtcg tatactgacc ggtgatctcc acagccatgc cgtttacgcg
     3241 tgtgacaaga atatttcctc cgcccactga agctccctgc acgctcaccg tatgtccctg
     3301 cgcatccgtc aaagtgataa gcgcggtatt ggggtgtgca ttttccaaag cagccggcat
     3361 gatggtgatg gccagcccgg ctttctgcgc aagcgtcaaa ctgtcgcgca ggcgcgcgtc
     3421 atcgggcttc atgcccagta tccccgcgac aagcgcctta tccgtcccgt ggccccggta
     3481 tgtcttggca aaactgccgt gcaaacgaat gtccgccgtc acggccggag cacccaaaag
     3541 cgcccgtgcg atgagcccaa tacgcgccgc gcccgcggta tgtgagctgg acggcccgat
     3601 catcacaggc ccgacaatat caaatacatt catggaaccc ctccacatta tgttttacat
     3661 atgcgctgcc gccatttgcc cgcccctctg aacatcgctc tgcggcttcg gggttattat
     3721 actacattta tccctttttt gatatgggaa atatgtatca tccgcaacca aaaaggcggc
     3781 gctcccgtgc agaaacgccg ccatgaatgg gtcaaatttt gattttgagc cacttgtcat
     3841 gtttgaagcg ccacattgtg agcaacaggc gcagaagctg atcgatggcc aagccgagcc
     3901 atgcaccgaa cagccccatg cccagcggat aaacaaacag ccagccggac agcggacgga
     3961 tacacgccac actgataaga gaaaccagcg ccacgaaacg cgtatcgccg ccgccgcgca
     4021 ggcacccgct gtaaatcacc tgagatatct gcaggaacac gatgaccgcg acgaaatcca
     4081 tgatcatggc gccgtaatcg aggatctccg gatctgctga aaaaaggcgg aacacatcgc
     4141 ggccaaatac aacatacac
//
LOCUS       DAWBSB010000197         4154 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_3661_length_4154_cov_3.045621, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000197 DAWBSB010000000
VERSION     DAWBSB010000197.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 4154)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4154)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4154
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3661_length_4154_cov_3.045621"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          766 a         1248 c         1227 g          913 t
ORIGIN      
        1 cctttggtaa acaggggcgg gccggcacag cggagcggat atagcatgcc cggatagcgg
       61 agggcagagt accgtaagga ggctatatct gttatgaaaa agatcctgtt tgctctggct
      121 tctctgtgct ctttggttct cgccagcggc gctttcttct gcgtaagata agtacttgag
      181 cgggccggac acggcctgtt tcccggagcc ctgccggccg ccctgttttt tggaatttac
      241 ggcgcttgtg tgcgtcatct gggatatatt tatataaggt atatggaaat ctgcggcgcc
      301 gccctgcggc gtcaaaaaac ggcgcgtttt gcgccgggct gtgtgtggcg cagacctgcg
      361 ccgggaggaa aacggctatg gacgaaaaaa agctgcgggt gtatacggcc gccgtctcca
      421 ttgcggggct gtcggttttc ctgctctccc tttgggaggt atatccttat tatctgccgg
      481 gcagcagccg cggggatatc tggagctaca gcctgatgat ggtgctgctg atggtggtgt
      541 gccggatgct gcccatttat ttcgcggagg acaaaacgct ggagatcagc ttcgtgcccg
      601 tactggcctg cgccataacg ggcggcgcgg cgctggcggt ggttctgttc ggggtatcga
      661 cgcttttcac cgtgatgcaa aacccagcca ccaagcatta ttactggcca ttggcccgcg
      721 agccccgcaa ggagctgttc aacaccggga acatccttgt ttccatcagc gcgggcgggc
      781 tgccgctgct attggcgggc atgccggata acctggcctc tctgcccgcc tttgggcttt
      841 ccctgctgtt cgccgcgctg tgcatcctcg tcaacctgac gctgttcatc ctgtattacc
      901 aggtgggagg catgggccag ttcggcacgc tgatgcgcca gaatatcggc ggcctgctgc
      961 ccaacatcat cgccaccgtt ccgctgggac tgctgctggg catcgtcatc aacatgccca
     1021 acagctactt ttacatcgtg ctgttcatgg cgccgctcat gctggcacgc tattccttca
     1081 agctgtatct ggacagcaaa tccatgcata tgcgcaccat cgccgccctg agcatggccg
     1141 tggacgccaa agaccattac acccagggcc attcccggcg cgtagcgtat tacagcgagg
     1201 ccattgcccg cgccatgcac aaatctccca gcttcgtggc cgacgtcaag acggccgcgc
     1261 tgctgcacga cgtgggcaaa atcggcatcg acgacgccgt gctcaacaag cccgctgcgc
     1321 ttacggccga ggaattcgag ctgatccagc agcatcctgt aatggggcgt aagatcatcg
     1381 acagcatccg tttttccgac acggtaaacg acgcggtgct gtatcatcat caccgttacg
     1441 acgccaaggg ctatcccgtc gagggcccgg gccccggcga gctgccgctc tctgcggcta
     1501 tccttgccgt tgcagacacc tttgacgcca tgacgtcgga caggccctac cgaaacggta
     1561 tgtcccgcga acaggcgctg gctgtgatgc aggaggtggc cggttcacag ctcgacccca
     1621 aggtcgtgga ggtattcatt tctctggctc cagagctgga cgccgcacgc gagcctgaag
     1681 aaagcctgtt gtagcccgcg ggctgttcac gaaaggaggc gcacggcttg gtgctgctgg
     1741 cattcattct gtgcgggctg gccgccggcc ttgcccgcgg cggcagcctg cgcgcgatgg
     1801 cgcagcacga tatcgcgctg ctgtggctgc cggtggccgc ctatctgtgc aaggctgcgg
     1861 ggccgtatgc agcgcgtctg ttccccgcgt ttgcgtctta tgcgcccctg gccgtatgtc
     1921 tgctgcatta tggcctgctg tttatttttg tggcggcaaa cctgcgccgc ggcgtttgga
     1981 gttttgtgtt cggcgccgga gccggaatga attttgccgt tattgcgctg aacggcggcg
     2041 caatgcccgt ctccgcgcgc gtgctggagt cagccgcagg cacccgtttg gccgcgcagc
     2101 tcgccgccgg cgagatcttt gcctacgcgc ctgtcacgga cagcacgcgt cttgtttttc
     2161 tgggcgatgt tctggcgctg gagccgttcg ggcgcctgct gggctttgcc agcatcgggg
     2221 acctggtgct gggagtgggc gcggcgatgc tttgctttac gatgctgcgt cccgcaaaac
     2281 attcggtgtg accgctgtct gtacgcacgc cgtccctgcc ggtaaacaaa gcggtgcaga
     2341 aaacgcgggc ggtgctccgg ctataagcgg cccgttccgt tcgccgcggt atctcccagt
     2401 tgggaccgcc gttccttgcg gcctaacaca cgttttgtgg gatgtaccgg aacaaagcgc
     2461 cgattgtttt caacgcgcgg ttttatttat gaacagctct ataatgctgc aaaataaaag
     2521 gccccgatgc acggacagcg aaacagtccg tgcaccgggg ccttggcgta tccttacaca
     2581 ggaacttccc catgcgcctt ttcatactca ttcacatatt tttccaacgc atactccatc
     2641 tctttattgg tagagcgctt attctctatc gcaatgattt tcatctttgt gatcaaagtc
     2701 tcagaaatgc gcagggaaaa cggtgcataa tagtttccca cggcacagcc tccattcaaa
     2761 gtgatatcaa aagtatatcg taatcacttc aaattatccg aatgcatatt gatatcaaaa
     2821 cgaattcatg ctatgcttaa gaggaaagtt ctggaaagga gtgctgcggc aatgggccca
     2881 caggacctgc tggatgcgtt ggatgagatc gtttatgtga gcgacccgaa tacatatgaa
     2941 ttgctttacg ccaaccgccg ctgccgttct ctgctcccca cccgggatta caagggcaag
     3001 ccatgctatg cactgctgca gggccagccc gccccttgtc cttactgcac caatgcccgg
     3061 ctcacaccgg acaaatttta cgtttgggaa ttcaacaacc cttccatggg gcggcatttt
     3121 cttttgaaag acaaactgat tgattgggac ggacgtccgg cgcggctgga gatcgcggtg
     3181 gatatcaccg aacaggagct gctgagccgg gacaccgccc gcaagctgga gaccgagcga
     3241 acattggtgg aatgcatccg ccgcctgatg gacgcggcat cctcccgcga ggccgcgcag
     3301 gccgtgctgt cctgtgtcgg cggtttctat caggcatctc acgcgttcct gctggagctg
     3361 catgcttccg gcagaagcgt gcactgcacc cagctgtgga cggccgccga cgcggaacct
     3421 ctggagcttc cttcgatctc cctgtccaac gaatcgctgc tcaaccggtt gcttgaatcg
     3481 cctcaggcac aatgtatcct tgcggaccgg ctgggccctg aataccggca ggtcgcggca
     3541 cagatgcagg atgccggggt cagcgccctg ctatttgcgc cgctgccgca cacgttcggc
     3601 cgcggcctgc tgggcgttgc aaatgccgcc tccggcatta acgatacacg cgtgctggaa
     3661 tcgctggcat actttgtcgg ttccgaactg tcgcgccggc gcctgcagtc ccgtctggaa
     3721 taccaaaacc gtcacgacgc gctcaccggg ctggaaaacc gtctgcaata ccagcgtttt
     3781 ctggccggtc ccattcccgc cggccccatc ggcgcggtat tctgcgacat caacagcctc
     3841 aagcagatca acgaccgcta cggccacagc cggggcgacg ccgttatcat gcagacagca
     3901 gacgtactgc gcgcctgctt ccccggcgcg cacatctacc gcatgagcgg tgatgaattc
     3961 gtcgtgctgt gcccgggtgt gacgcaggcc cggttcgaga caatgctcgc gaaagcccgc
     4021 gaccgccttg ccagtgaaac agactacggc gtttcgttgg gcggagcctg gcacgcctgc
     4081 gcccgggaag atcttgacga gctggtgcgc gctgccgacg agagcatgta tgcggacaag
     4141 caggagcatt acgg
//
LOCUS       DAWBSB010000198         4154 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_3660_length_4154_cov_3.915345, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000198 DAWBSB010000000
VERSION     DAWBSB010000198.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 4154)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4154)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4154
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3660_length_4154_cov_3.915345"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          863 a         1161 c         1328 g          802 t
ORIGIN      
        1 cgccatactg aaagtgatgc ggtctttttc aggcatgctg cccatggtga gccattttga
       61 ttttggctga agccgcagta ccagcacggc gtaatgtgtc tggctgttca aagggatacc
      121 caggaattcg atgctttgca gaatgtcctc ctcgctttcg tcattgttct gcaaaagatc
      181 gcacaaacgg cgctggcgca gcagcggcat gctttgttcc agctttcggg ccatgtcttc
      241 caggaccagc tgctgggaag acctctggtc cagcatggcc accactttgg tgatcaccgc
      301 gtccagttcg tcaatgtcga tggatttgag gatatagtct acggcttcca tcttcagagc
      361 atcacgcaga tatcccacat ctgcatagcc gctaataaat atgattttga cgccgggata
      421 gctctccagc acatttttgg ccagtgtgat gccgtccata tgggacatcc gcacgtctgt
      481 gacgatgatg tcaacttcat tccggcggac aaaatccagc gctgggacgc cgtcgtcgaa
      541 taccccggcc acttccacac cgtgcttgcc ccagttaaag tgcgttacaa gcccacggcg
      601 aaccgtttcc tcatcgtctg cgataatggc tttgtacatc tttaccgttc ccttccgcgg
      661 ttttgccgcg cgctcaaagt gtttgcaggt attcttcgat ccacgggatg gccgagccca
      721 gggcggccgc ttctttttta taggcacagg ccttgagata gccggcgtcc ggctcgaagg
      781 tgttgcggaa ggccgcgcgg cggcgcagcc cggccagctc tgcatccatg tatcggccta
      841 catagcctcc aagaatcacg tcgcagtcca gcatcatgcg caggttgttc actacgacgg
      901 ctacgttgtc cagataagcg tcccacacgc tgcgcagcgc ggcgtcaccc gtgtgcagcc
      961 gcaggaaaaa atctttcagg ctgccgccgg tgtggccgtg cagcaggccg gcgcggcagt
     1021 aggcgtccac acagccgctt ttgccgcagt agcacggccg tccgccggga accagcgtgg
     1081 tgtgccccac ctcgccgctg cgcaggttgt gccccagata cagcctgcgg ccgatgagca
     1141 ccgcgctgcc cacgctgttg ctcagggaga gataaaccgc gtcccgcaga gagacgtcgg
     1201 cccaggcctc ggcaaggcag ccggcgtttg cgtcgtttat catgcggcag ggcaggccga
     1261 gatattgttc gaattcatcg gcccgcagtc cctgaatgga aagcgcgtgg gagcgcagga
     1321 cgaggtcctg cccgctgccg atgatgccgg gaatggacag gccgacgccc aaaaccgatt
     1381 gccggggcag ctccgcgcac agctcgctga tgaacgcggc gagcgcccga aaataggcgg
     1441 gcgtacgcgt aaaggccaga gtgcgggttt gcgtgcgcgt cacctggccg gagagatcca
     1501 cagccaccag gctgacatgg cgcagagtga tgtcggcccc gacgacgtac cgcgcgccgg
     1561 gacaggcgct ccaggcccgg ggcttgcgtc cgccggtgga actgaattcc ccgcagggct
     1621 gcaccaggcc gtggccctcc aggcggcgca gcagagtaat ggccgtgggc gtgctcaccg
     1681 ccagagcgtt cgccatctcc tgtgtggaaa aacgttcttt ttccatggcc catgcagccg
     1741 cctgcgccgc ccgcccgcgc tgtgcgccga aaagatttac tgtctccatc ctgagtgcct
     1801 cccgcctgtt tgtggtttta ctgtaacaca ttcaaaacac tttgtaaaat cgttttaaaa
     1861 actttacaaa aagaaagact cattcttgga gattccaaca aaattggaag agaccgcttg
     1921 acaacgcatc aaaaaagacg cattctaatg ctggaatctt tttaaactga gttaaaaaag
     1981 ttttgggcat gggaacatgc ggaaaggatg gatcgacgat gaagggagct ttctttctgg
     2041 gcaaccggga atttgaagtc cgggatatgg aaaaacggcc gctggccgcg gacgaagtgc
     2101 gcattcgcgt gatggcgtgc ggcgtgtgcg gtacagatgt ccacatttac cacggcgagg
     2161 cgggctcggc cgaagtgacg ccgccggtgg ttctgggaca cgaatatgcg ggtattgtag
     2221 aagaagtagg cgcggatgtg aaaggtttgg cgccgggcga tcatgtcgcc gtagacccca
     2281 atatttactg cggcgcctgc acgccctgcc gggacggtaa aaagcagttc tgtgagcatc
     2341 tgcaggcgct tggcgtcacc cgggacggcg ggtttgcaca gtacagcgca gcgccggcgg
     2401 cacagtgctt caaactggca ccggagctgc cctttgaggc aggagccatg gccgagccgc
     2461 tggcgtgctg cctgcacggt atcgatgccg cgggtatacg gcccggcagt acggtatgtg
     2521 tgatcggtgg gggagccatc ggcctgctga tggtgcagct ggcccgcctt tccggcgcgg
     2581 cgaaggtcat tctcagtgag ccggtggaac tgcgccgcag cgccgggctg tccgttggag
     2641 cggatctggc tgtcgacccg ctccgggagg acctgtccgc ccgtgtgcgg gaggctgcgg
     2701 gcaaaagcgg cgcggatgtg gtgattgaat gtgtggggaa cacggcggcc acacgcagtg
     2761 ccttcgaggc cgcgggcttc ggcgcgacgg tgctgctgtt cagtgtgccc gcgcctacgg
     2821 ctacggttcc gctgccgttg ttcgacgtat acaaaaaaga gcttgccatc cgcggctcct
     2881 tcatcaaccc ggatacgcat cttcgggctg tggaattgct gagtgcgggg cgcatcaatg
     2941 tggagccgct catcacccac acgttcggca tcggggaaat ggagcaggcg atcaaaatgc
     3001 agatgggcgg cgaatccatc aaagtgatgg tgctgccaaa caaacgctga acgcgtacaa
     3061 acgggagagg atgattcaga atgcagaaaa tgctgcagca ggtgatgacg gcgccggggc
     3121 acatcgagtt ccgggaggcg cccgtacccg cacccaacgc tggcgaggta ttggtaaaga
     3181 tcatgaagat cggcgtgtgc gggtcggata tccatgtgta tcacggcgag catccgttca
     3241 caaaatatcc ggtcacccag ggccacgagg tgtcggggaa aatcgcggcg ttgggcgagg
     3301 gcgtgacggg atttgccgta gggcagaagg tgaccatcga accgcaggtc tactgcggcc
     3361 ggtgccatcc gtgcacgcac ggcaagtaca atctgtgcga ggaacttcgg gtgatggggt
     3421 tccagacgac aggcacagcc agtgaatatt ttgccgtgga cgcgggcaag gtgacgcctt
     3481 tgccggaaac gatgacatac agcgaaggcg cgatgataga gccgctggcc gtgacggtac
     3541 atgcggtgcg gcgcgccggg gatgtgcgcg gacagaagat caccgtgctg ggcgcggggc
     3601 ccatcggcat cctgctggcc cagagcgcaa aggcgctggg cgcggcgcag gtgcttatca
     3661 ccgacatcag cacgccgcgg ctggaattgg caaaagcatg cggcgtggat tttgcagtga
     3721 acacaaaaga gcacggcttc ggtgaagcac tggaagaatg cttcggcccg gacaaggcgg
     3781 atgtgatcta cgactgcgca ggcaacgata cgaccatggg acaggccatc cggcacgcgc
     3841 gcaagggcag cacgatcatc cttgtggcgg tgtatgcggg tatggcaaat gtggaccttg
     3901 cggtgctcaa cgaccatgaa ctggatctga acacgagcat gatgtaccgc catgaggact
     3961 atcttgaggc aattcgcctg gtgaacgaag gcaaggttcg gttggagccg ctgatgagcc
     4021 ggcacttccc gttccgggat tatctggcgg cataccagta catcgacgcg aaccgggaga
     4081 cgacgatgaa agtgcttatc gacgtggacg agagcgaggc gtgaacgtgg gaaaaatcgc
     4141 ggaaaccata aatg
//
LOCUS       DAWBSB010000199         4147 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_3670_length_4147_cov_3.630254, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000199 DAWBSB010000000
VERSION     DAWBSB010000199.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 4147)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4147)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4147
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3670_length_4147_cov_3.630254"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1394..1403
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          878 a         1225 c         1066 g          968 t
ORIGIN      
        1 gcctcaatat ccccgcgggt gcaggccgcc gacccctgct gcatttcccg gccgccgccc
       61 ctgccgccaa gcgcggcgtt catctcttta cacagagcgt gcacatctgc ccctccggcg
      121 ctgcccaacg catacttgta gcctccggca tccgtgcccg aaaaagccgc gcacacaccc
      181 gtgcactgct ccatcagtgc cgtacacagg cggcgcaggc tgtcggggga aaggtgtggc
      241 tcgaatacca gcgcattttt tctgccgcgg aacgcggcgg cgtccgccgc aaacagacgg
      301 gtctccagcg cggccttttc cgctctgagc gccgcgtttt catccgccag gcgctgcacg
      361 gcagccgctg tttcattgat cttcgcgctc agcagaccgg agactgcctc ggcgttctga
      421 aacttgatac agtgatcctc atacgcccgc ataccgcaca gcatggtaac gcgggttccg
      481 cccttatagc tttgagcgga cgtcagcttg atgctgccca cctggccgca gcgttccaca
      541 tgcgtgccgc agcaggcaca gacatccaca ttcgctaccg tcacgattcg tatcgcgccc
      601 gtcagctcct ttttgctgcg gtagttcagc tgcgccagct ccgaaggcgc gggccaagct
      661 atcgtcacgg gcgcgttctg ccatatcacc cagttggcag cgcgttccac atcggccaac
      721 tccgcatcgg tgagcggacc ggaaaaatcc accgttacat cctgcgcgcc gatatgaaag
      781 cccacgttgt cgtagccgaa ctgagcgtgt acgatgccgc tgacgatatg ctcgcccgtg
      841 tgctgctgca tgtgatccag cctgcggcgg ccgtccacat caccttggac catctctccc
      901 acatgcagcg gtgctgtgac cgtatgcacc actacaccgt ccttttcgtg cacatccaga
      961 acgcgcgcac cgcccaaggc tccccggtcc gccggctggc ctccaccctc ggggtaaaat
     1021 gccgtggcgt ccagcgttac ggcaaaattg ccgtccgtgg gcacgcagct cagcacagta
     1081 gcgcagaact gtaaacaggc gccgttttcg taatagagct ttttcgtctc tgccatgccg
     1141 ttttctttcc tttcaatgaa caggccgatt tttgatatcg gggatattgt aacataaaaa
     1201 ttcagcaaaa gcactaaaaa aagcgccaac ttttcgttgt ccgaaacccc ggaaattgta
     1261 ctataatgca tataaccggg ccgccggagg caaagctctg aaacagagcc tttccgcggc
     1321 ctgcggtgag caaccgcccg gcgcttgccg ggcagcaagg gaggaaacca tgcgcaaaag
     1381 cggcatcctg atgnnnnnnn nnnctgatgc cggtggcgag cctgccgggg ccttacggca
     1441 tcggctcgtt gggcaaaccg gcattcgtgt tcattgattt tctgaaaaag gcggggcaga
     1501 aagtgtggca aatgcttccc ctttcaccta cgggctttgg ggacagcccc tatcaaagct
     1561 gttcggcctt tgccggcaac ccctatttta tcgatctcga tatcctgcgc aaaaaaggtc
     1621 ttttgaaaaa aggcgagtac ggggcaaagg aatgctgcac caacgagggc gcagtggatt
     1681 acgcggctct gtaccacagc cggtacggta ttctgcgcaa ggctttcgcc cgtttttcca
     1741 cttggtaccc ggacgactac taccgcttct gctacgagca gggctggtgg ctggaggatt
     1801 atgcgctgta tatgacagcc aagggcctgt atgaagggaa aagctatctg gagtggcccg
     1861 agcccgtgcg ttcccacacg ccggaggcca tccggcagct gtatgccgag catgaggcgg
     1921 aagttcattt ctggaaattc tgccagtacg agttcagcct ccagtggacc gccctcaagg
     1981 cctatgcagg ggaaaacggt atcgagatca tgggggatat ccccatctat gtggcggcgg
     2041 acagcgcgga cgtatgggcc gcgccggagc tgtttctgct ggatgcctgc ggcgccccaa
     2101 aagaggttgc cggctgcccg ccggacgcat tttcggacgc aggccagctt tggggcaacc
     2161 cgctgtacga ctgggcccac cacgaaaaaa ccggctttgc ctggtgggtg cggcgcatcc
     2221 gctatgccct gaatttttac gatattctgc gcatcgacca tttccgcggc ttcgacacct
     2281 attatgccat cccctacggc gcgaagaccg cccaaacggg cgagtggcgc agcggcccgg
     2341 gcatgaagct gtttcgcgcc gtgaagcagg cgctggggga tgtgcccatc gtggccgagg
     2401 acttgggcga gctgtttccc agcgtatatg agctgttggc ggagagcggg ttcccgggca
     2461 tgaaggtact gcaatttgct ttcggccccg gagacagcga gtatctgccc cataaccatc
     2521 ccgtacactg cgtggtgtat acgggcacac acgacaacac cacggctgcc gcctggtaca
     2581 ggaccgccgc cccggcccag cggaagaaag ccgccgctta cctggggctg aaccgggagg
     2641 agggctttgc cgccgggctg gtgcgcggtg cgctggccag ccccggagag ctgtgcatta
     2701 tccctctggc ggattatctt gccctggggg ccgaagcgcg catcaacacg ccgtccacgc
     2761 tgggcggctc gaactgggtg tggcgcgcgc tgcccggcca gctctgcgcc gccaacgcca
     2821 aaaagatcgc ggcgctggcc gtactgtacg gccgcgcctg acattaaagc acagcaaaaa
     2881 aaacagggcc gctgacatgg gcggcatccc gtaaagaagt taagggcaag gcagaccaca
     2941 atggtttgcc ttgccctttc atactgcgta gcccactatg atgctttcgg ggaaagtgcc
     3001 cgtggaaaaa acggtaaccg gacgcttcat ttgccgattg caatttctac atattggctt
     3061 ccgacaacag ttggcctatg atttcttgtt tgcttgcggt atagcgcttc cggtcgtctg
     3121 caaaccgcac tgctgatttc cgcttgaact cgtcgtacat catcgctttt tcagggtttg
     3181 cgttcagata gtcccggaaa ctgatataat tactccaccc tgttccgttc cttttcacga
     3241 tatggatatg gtgtgttcgt atatcgccat cttccattac aaaaaataat tctcctgcaa
     3301 cagctccttc atggaaaaaa atattatgct gtttcaggac ttcaatataa ggcaatatat
     3361 cctttatgtc gtggacagca acggcaatat caataatcgg ctttgcgtga attgagcaaa
     3421 tggccgtact tcccacatgc tgaatatcaa gtgcagtatc tcccaagagt tgctttaata
     3481 cgctgattac attttcagca ttttcatgcc attcctcttg atgctgcacc aactttaccg
     3541 ttccgctttt taaccctatc ataacaatgc tgtcctctcg agaacccgag tcgttggtca
     3601 atttattata aaacaacacc cgccgaaaaa caactttgtc agcgggtgtc agcttcctta
     3661 tgatagaccg cccaaagcgc ggccctgttt tttcatcgtg tttcccgcac tgctctcagc
     3721 agcctttctc gtaacgcatg tatgcgacat cccagcccat ggagtactcg tatgcagcac
     3781 acgcttttac aagatagttc agaaaattct ttttcataac agtaccctcc tttaaaggta
     3841 tctctaaaat ttcccgtccc tcaatcggac ggcttccgtg tcccctttcc cttgggacat
     3901 ttttattata tgccgtcatt ttgcataaat caatagcttt attattgtga tattgcacaa
     3961 actttctgtt ctttgtcaaa gccgtgtacc cttataaaat gatgtttttt tcacaatgta
     4021 tctgaaaacc cgcccagcag gccgtgtaca taattgacaa aaatataact atattatcgt
     4081 catattgacg aatttcctgg aaattttttt gccttttgca aaatcagttc cttcatatgc
     4141 cctttct
//
LOCUS       DAWBSB010000200         4134 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_3682_length_4134_cov_2.943368, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000200 DAWBSB010000000
VERSION     DAWBSB010000200.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 4134)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4134)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4134
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3682_length_4134_cov_2.943368"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3336..3345
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          852 a         1261 c         1380 g          631 t
ORIGIN      
        1 cgccaccgcc cgcaagggcg cgcagatggc cttcatcccc gccggtatgg gcggcggcac
       61 gggcacgggc gccgcgcctg tggtggccga agtggccaag gagcttggca ttcttaccgt
      121 gggcatcgtc acgaagccct ttgcctttga aggcaagcgc aaaatgaaca tggccgagat
      181 cggcatcgcc ggcctgctgg agcgtgtgga ttccctcatc gtcatcccca acgagcgcct
      241 gaagctcatc agccaggaaa agatcacgct gctgaacgct tttgaagcgg ccgacaatgt
      301 gctgcgccag ggcgtggaga gcatctcctc gctgatcaat gtggcggctt tcatcaacct
      361 ggacttcgcg gacgtgcgca gcatcatgaa ggacgcgggt tacgcgcaca tgggcgttgg
      421 ttccgccaag ggcgtgaaca aggcggagga cgcggccaac gcggccattt ccagcccgct
      481 tttggagacg agcatctccg gcgcacgcgg cgttatcatc aacatcacct cctcgccgga
      541 catcggcctg gacgatgtgg aaaaggccgc cagcctcatc accaattccg cacaccccga
      601 agcgaacatc atctggggcg ccgcgttcga cgagaacctg aaggatgaga tgagcattac
      661 cgtcattgcc acaggctttg acgcgggcct tgtgggcagc gaccccatgg acgtgccgca
      721 gaatgcggcc acgttcggcg gcaaggtgca ggtagcgttc aagaacgata ctgcgcccgt
      781 ggccccggcg cagccggtgc agcccgttgc tcccacgcag ccggtgcagc ccgcccccgc
      841 ggtggaggaa gacgacaaca gctatttcga cgatatcctc ggcctgctga acaaccgcgg
      901 aaacaactga gcaaccttgg gaaaagcggg gcgttaagcc ccgctttttt ggctgaatgg
      961 cgctgcgtgc gccccggccg cagcacagac gaaaggaggc gcgcgtgtgc agacactgga
     1021 ggaaaaacag caggacggca tgacgggcgc cggcccggag acggaatttc ataccgagct
     1081 tttcggcttt aaccgggtgg aggtgctgtc ttacatcgag cgcatctccg cggcaaacgc
     1141 ggagaaagcg cgggcgctgg aggacaccat cgccgcgctc caaaaagacc tgacaggcgt
     1201 gcgccggcag ggaagcacac tggcgcagaa ggcaaagcag gtattcaacg agctggagaa
     1261 ccagaaaaaa cgtgcggagg acgcggtggc agaggctgcc gcgctgcgca cagaggtgga
     1321 caaggccaac gacgagatcg ccgcggtgcg cagccgcctc tttgcccggg agcaggaaaa
     1381 cgccgcgctg aaaagcgaca atgcgcggct gagcgaaacg gtggacgatc tgacgcgcgc
     1441 tttgacacgc cgcggcgcgc cgctgccgac cgggcagaac gcgtccgctt cgggaagcag
     1501 cggcgaccag gccctgctgc agcaggcgca gctcaaggcg cgggacattc tgcgcgcggc
     1561 tgacgctcag gcgacggcgc acattgaaaa ggcgaaagag gacgccgcag ccattgtggc
     1621 ccaagcaaag gtggaaaaga gtaaagcgaa ggcggggctg gcggagtccg ccgacggcat
     1681 tgcggccagc atcgctgtgc tgaaggcgca gcttgcggcg gtggacgcca agatactggc
     1741 ggcgacgggc gatctgcaga aagccaccga cggcatcact gccgcgctgg gcaataccga
     1801 gcgcagcctg tcctcgcttg gcgcgcaggt ggagcgtttc cctgaaaatg cgcccgccgc
     1861 acagccgcag ccggcccaac cggcagcgcc cccagcgccg caggagcccg ctccggcccg
     1921 tgaatacgct gtgcaggagc atgcggcgta ccctgtacgg gaggggcgga gcgttcggga
     1981 cggggcggtt cccgcttttg cgccggacag cgaatacgac cgatatgtgc gcgcccgcag
     2041 ggaagaagac gcctattaca gcgctctgcg ccgcgtgcgc tatgaagatg aactgcgcga
     2101 ccgtttttat gaagaggaac ggcttcgtca ggaggccctg cgccgcatac gctacgatga
     2161 agcgctgcgg gaacaggagt acgcgcgccg ctgcggcccg gaccatcgcg cgccttatgc
     2221 ggccgcgccg gagggcggtt atcctttgta tgcatcccgg agggacgacc ggtttgaccg
     2281 agcgcccgcc ctggggcgtg acggaggagc ggccgttgtg caggcgagag aggcggatct
     2341 cccgcagaca gcggccgccg gacggcacgc tgcagtccag cagcccatgg cgcccatgcc
     2401 gtcctctgtg ccgcccgcgg cagaggaacc gcagcaggag ctgcagccgc cggcctttga
     2461 agcctcccgc ccacgctacc gcgcaacgaa tggcaggccg ctgccggggc gcaacgtaac
     2521 gcctcaggga gacaggcccg ccgtcctgga ggagcaatac gacgcggtcc agcacgcggc
     2581 tcgcggcggt gcgccgggca atgcgcctta tgatattccg gaagcgccgg cgcagcccgt
     2641 ggtgcagaag gtggagatcc cgcaggtgcc gccgaaggag ccgccggaat ccggctcaca
     2701 gatcccgttt ggaaaaccgc atgcaccgcg tgccatgccg tttgccccgt ttgaccccta
     2761 tacgccccat accgcgccct atatcgaacc ggacgcggag gcggcccgca ttttgaacac
     2821 gccgccgccc ccgccgccgc gtgggacgga ggcgaaacag ccctatgtgc cgcagcccac
     2881 gccgatcgcg cccagcgttc cgttgtcggg cccttatgcc aggccgtccg ccgcggcggt
     2941 ggcgggcacg ccgcaggcgc agcctgcgcc cgcgcgcgcc gaagagcctg tgatcatccc
     3001 ttattccgcg cccatccctc cgatgggaag taaggcggat gcgggagaag cggccgctgc
     3061 ttcagcagcc cggaaggggg aggcttcccc ggcggcgcct gtatccgggc agaagaagcc
     3121 ggaaatgcag cgcgcgcagg agccttccgc gccgtctgtg ggagctgccg cgaaacctcc
     3181 ggtgttcact gcgccgcgcg gccagcaaaa gcaggggtcc gcgctgtcgg acgacctgct
     3241 ggccaatttg aacgcgctgc tcaacggagg gcaggaaccg taaggccgtc cgggccgaag
     3301 ccttaaaata agggcaacac cgcacaattc taagtnnnnn nnnnnttttt gtgacagttg
     3361 ccggaaaatc tgcaagcgag gtgacgctta aggcatcagc cgtgaattgt gcggtgttgc
     3421 ctaagaccgc atctgccgcg cgcagccgca tatctttcgg cggcgcgtac gcaggtgcgg
     3481 tttattttac gttcgagggg ctccttcaac aacaaaagca gccgaggctg tttgccggcc
     3541 gcataaacag atgcgccgcc gtgactattt ccataggggc cggcgcaaac tatgaaaaga
     3601 gccgtgcctg ggaacagaaa taggacttgc atattttata ctaaaatggt atagtattat
     3661 taaggaaaaa gaaaccaatc ccgccggcgg tgaaagcgcc gcacagcggg cgataacaca
     3721 aaaggagata agattatggc ggtaaatgtg aaaacacagg attttgaaga acaggtggca
     3781 aaggcttccg gcccggtgct ggtggatttc tgggcgagct ggtgcggtcc gtgccgtatg
     3841 ctggcgccgg tcattgacca gcttgcggcg gagcagacgg atgtaaaggt ctgcaaggtg
     3901 aacatcgacg aagaaccggg ccttgcccag cgcttcggcg ttatgagcat cccgacggtg
     3961 gtgctgtttg aaaacggcca ggaaaaaaac cgcagcgtgg gccttgtgcc caaggaaaag
     4021 ctgctggcgc tgctgggacg ctgattggtc tgaggaatcc ggaggataag acagccttca
     4081 ggatggtttt tatgttttga ggcagtaaaa aggccgcgtg ccctttcaca aggg
//
LOCUS       DAWBSB010000201         4117 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_3693_length_4117_cov_2.493599, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000201 DAWBSB010000000
VERSION     DAWBSB010000201.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 4117)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4117)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4117
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3693_length_4117_cov_2.493599"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          899 a         1135 c         1219 g          864 t
ORIGIN      
        1 cccgtaatga gggccgtaga ggcgcgccag tcggtaaagc ccagcggcgc gaacacgggc
       61 gcaatgaccc tgccgatgcc cgccagcata ctggcggagc tgtccgctac cacgttgagg
      121 cggatgtcga aggactgcaa aaaccagatg atgatggtgg ccacgaaaat gaccgtgaac
      181 gcgcgggtga gaaagtcttt ggccttgtcc cacagcagca gccccacact tttggggctg
      241 ggaagacgat agttgggcag ctccatcaca aagggcaccg gattgccgcg gtacatcgta
      301 tttttcatca aaaggccgat cagcaccccg atgaccatgc ccatcacata cagcagaatc
      361 atcaccagag cgccgttttg ggggaagaac gccgccgtga acaccgcata gatgggcagc
      421 ttggcggaac agctcatgaa gggcgtcagc aggatggtca ttttccggtc ccggtcgctg
      481 gaaagcgtgc gcgtggccat gatggccgga acgctgcacc cgaacccgat gagcatgggc
      541 acaaagctgc gcccggaaag gccgatcttg cgcagcagct tgtccataac aaaagccacg
      601 cgcgccatgt agccggaatc ttccagaatg gaaaggaaga aaaacaatgt tacgataatg
      661 ggcaggaagc tgagcacgct gcccacgccc gcaaacacgc cgtcgatgat gaggctgtgc
      721 accacggggt tgatgccgta agcggtgagc ccgcggtcca caagagacgt cacagcatcg
      781 atgcccagcg agagcaggtc gctcagccag gtgccgatga cgccgaacgt cagccagaag
      841 atcagaaaca tgatgcccag aaaaatgggg atggccgtgt atttgcctgt cagcacgcgg
      901 tccagccgca tgctgcgcag atgttccttg ctttctccct ttttcaccac ggtctgggcg
      961 cacagctttt caatgaacgc atagcgcata tcggccagcg cggcttcgcg gtcggtgccg
     1021 agctcggctt ccatttccgc gacggtgtgg ccgatgagct ccacctcatt ttccgaaagg
     1081 cgcagcatgt ccatcatggg cttgtcgccc tccaccagtt ttgtggcggc aaagcgcatg
     1141 ggcacgcctg cggcctcggc gtggtcctcc acgatgtgcg ccagcgaatg gatgcagcgg
     1201 tgcaccgcgc ccgaacagaa atcctgccgc ttgggcagca ttttcagctg tgcggcgcgc
     1261 agcgcgtgct cgatcagctc gtcgatgccc tcgtttttcg cggcggagat gggaaccacg
     1321 ggtacgccca gctcctcctc cagcttgttt acgtccacag tgttgccgtt ggcgcgcatt
     1381 tcatccatca tgttcagcgc gatgaccatg ggaaggttca gctcgatgag ctgcatggag
     1441 aggtaaaggt tgcgctcgat gttggtggcg tccacgatgt tgatgatacc atccggcttt
     1501 tccttgatga gaaaatcccg ggtgacgatc tcctcgctgg tgtacggtga aagggagtag
     1561 atgccgggca ggtccaccac ggtggccatc tcgcacacag cgggagccgg cgttttggga
     1621 tgaccccagc tgcggggacc gtggcggtgt tcgcaggtat tgtgtccgcg gatgacgcct
     1681 tcttttttgt ccactgtgac gcccggaaag ttgcccacat gctggttgga gccggtgagc
     1741 tggttgaaca gcgtcgtctt gccgcagttc tggttgccgg cgagtgcaaa taccattatt
     1801 ccgcctcccc gtccggcgct tccagcgcaa tttcccgcgc gtcgtccagg cgcagcgtaa
     1861 gctcatagcc gcgcaggcgc agctcgatgg ggtcgcccat tggggctacc ttctgcacgg
     1921 tcacctgtgt tttcggcgtg aggcccatgt ccagcaggcg gcggcgcagc gcgccctgtc
     1981 cgcccacggc agcaatgcgc cgcgtctgcc cgacgggcag tttgtccagc gtcatatccg
     2041 tttcccccat tgctccaaat gcaaaatgtt agcctgagct aactctctgc tataagaata
     2101 cacgcgcggc gcggggatgt caagccggga aaacgacaaa ataaccgaaa agagcaatta
     2161 aattttgtgc aagccggatg cggacggtcg aaaccgccgt ttggccccgg ctgcaggaaa
     2221 acgccgcaaa accttgacaa aacgccctct cagggcataa actaataata gtaaagctcc
     2281 tgctcggcgg ggacttcgtg aaccgaagaa tccttggacg cgcgggggct ttttctgcgg
     2341 cggaaccgca aaagctgaca caaaaaggga gtgacagtgc gtggagggcg atcctcgagg
     2401 gcgatcatgc ccgcaaaaat tgaacagcgc ttcgcgcacc gccgttttct gatctctgcg
     2461 gctgtgcgtt tcggcgcggt aagcagggat attgtatgat ttcgatttat cttaccgacg
     2521 atggcaagct gcgcgagctg gagcagatcg tgcccggcgc gtgggtgcat ctgtgcgcgc
     2581 ccaccgagga agaagtggca accgtgtgtg ccaagctgtg cgtcgagccg gacctcgtgc
     2641 gcgccgcgct cgatgaggaa gaacgcagcc gcatcgattt tgaggacggc catatgctcg
     2701 tgctggtgga tacgcccacc gtcgaggccc agcagggccg cggctttgtg tattccacgc
     2761 tgccgttcgg catcatcctc accgaggaga acatcatcac cgtatgcctg aaggaaagcg
     2821 ccctggcgcg ggccttcagc gaaagcccgg tgaagaattt ttccaccaaa aagaaaaacc
     2881 gcatgacgct gcagctcttg taccgcaacg ccacgctgtt cctgttctat ctgcgcgagg
     2941 tggataaggc cagcagccgt gtggaaaacg aactccatct ttcgatgaag aacaaggaac
     3001 tgatccagat gctggggctg gaaaagagcc tggtgtactt ttccacctcc ctcaagggga
     3061 acgagatcgt gctggaaaag ctgctgcgca tggattttgt gaaggattat cccgatgata
     3121 ccgagctgct ggaggacgtt atcattgaga ataagcaggc catcgagatg agtaatattt
     3181 accgtgacat tttgtccggc accatggacg cgttcgcctc cgttatctcc aacaacctga
     3241 acatcgtaat gaaggtgctg gcagccgtta ccatcattct taccatcccg tccatcattt
     3301 tcggcctttg gggttccaat gtgcccgttc cgtttgaaag ctcgccctac ggctttttcg
     3361 ccatcattgg tatcgctgcc ctttgcaccg ccgtggccgt tgtggtcatg gtgcggaaaa
     3421 agtggctgtg accgacatat ttcgatgtat aatacacgcc gctgcctgca taatatgcgg
     3481 gcggcggttt tcttttttgc aatgccgcag ggggaataaa agaaacgcct ggtcctttgt
     3541 gcaaaaataa caggaaacgg aaaagaattt gagtgacttt gcgggtttta caattttttt
     3601 gtacaaaata gataaagata gatgctattt ttggcgtgac gttgcataat tgtaccagaa
     3661 aagccttgaa aactgtggaa gaaataggta ttattatagg taataaaagc tgtggcgctg
     3721 cacatttcat tcgtgcttta gccggaaggg ccggcgcctt atgtgccgcc tgcggggaca
     3781 acgccctgct gcggcggcgc ggcccagccc gcgcccggag agagggagga tgttcaaaat
     3841 gaaacagaaa gcatcgcctg ctgatgtacc ggtgtactta ttcaagcagg ggaataactt
     3901 cgagagccag cgttttttcg gcgcgcattt tgagaacgtg ggcgagaccg aaggcgtgat
     3961 gttccgtgtt tgggcgccgc gcgccaaggc cgtctccgtg gtgggggatt tcaacagctg
     4021 gatcccctcg gccactccga tgaaaaatat cgatccccag ggaggcatct gggagcgttt
     4081 cgtccccggc attcggcagt ttgatatcta caaatac
//
LOCUS       DAWBSB010000202         4112 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_3698_length_4112_cov_2.607099, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000202 DAWBSB010000000
VERSION     DAWBSB010000202.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 4112)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4112)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4112
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3698_length_4112_cov_2.607099"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          894 a         1082 c         1261 g          875 t
ORIGIN      
        1 atcacggtaa cggcgctgaa ccgttatgtg aaaacgctgt tggagcggga ctctgtttta
       61 acagatgtgg cgctgcgcgg cgagatatcc aattttacga accattataa gaccggacat
      121 ttttattttt ccctgaaaga cgagcaatgc tctgtcaaag cagttatgtt ccggcgtgat
      181 gcacagaatc tggcattcat gccgcagaac ggcatgcgag tcatcgtgcg gtgccggatt
      241 tctctgttcg agcgggacgg cgcgtttcag gtctatgtgg aagatatgtt tccggacggt
      301 attggtgcaa tgcagatggc attcgagcag ctcaaggaaa agcttgacaa ggaaggtctg
      361 ttcgctccgg agcacaagaa gcccctgcct gcaatgccga aatgcgtcgg cctggttacc
      421 tccaaaacgg gcgcggcgtt gcaggatatc ctcaacgtga cgcaaaggcg ctatccgatt
      481 gcacgttttc tgctttattc tgtaaccgta cagggaaacg aggctgctcc ggagatcgca
      541 aacgccatta cgcggctgga caagggcgga cgtgtggacg tgatcatcgt tgcgcggggc
      601 ggcggctcac gggaggatct gtgggttttc aacaatgaga gtatcgcccg cgcggcattt
      661 gccagcagta cgcccgtagt ctcggccgtg gggcatgaaa ttgatttctc cattctggat
      721 tttgtggcgg atctgcgtgc tcctacgcct tcggcggcag cggagctggt gatgccggac
      781 atggaagcgg aattgcggga gattcggcgc agttattcaa atatttgcaa agaaatacaa
      841 aaccgccttc agttatgtta taatagagtg cgggatatag aggcggcgcc ccagcttgcg
      901 gcggcgcgtt cgctgccgct gggcctgctg cgggagcttt cagaaaaggc tgacgccgcc
      961 cgggctgcca tgcatggcaa aatggacgcc gccaaggggc gcgttgctca cgcagccatg
     1021 ctctgcggtt cgctcagtcc atacagtgta ctggcgcgcg gttacagcat cgcccggaaa
     1081 gcgggctctg tgctcaaaag cagcgcggac gcttcggagg gcgacagggt agaggtacag
     1141 ctggccgcag gacggctggg ctgcagggtg gaaacgatta ttacagagga agagctatga
     1201 aaaaagaaat gacatttgaa gcggcgggca cggagttgga cgctatcctt gcggagcttt
     1261 ccgactcgga gacgcctctg gaccgcagcc ttgtgctgta cgccagggcg gcagaactca
     1321 tcgccttttg caacgaaaca ctgcaaaagg cgcagatcac cattgatgag atcgacgcaa
     1381 aaaacgccgg gaagcaggaa taaagaagga gcgttctatg gattacaacg cgcagtacca
     1441 ggcatatttg aacacggccg agacggcgct ggcgcagcag gcggaccggt gctttaacga
     1501 acgctcccgc gtgtgtgagg cggcgcggta cagcctgttc ggcggcggca agcgtgtgcg
     1561 cgccgtgctt tgcctggccg tgtgtgatat gctggacggc aacagaaacg cggcagcctg
     1621 ttatgcggct ggggtggaga tgctgcactg ctattctctc atccatgacg accttccttg
     1681 catggataat gacgatatgc gccgagggcg tccgtcgtgc cacaaagctt ttggggaggc
     1741 tacggctctg ctggcaggcg acactctgct tacggcagca ttcgaaactc tttccggttc
     1801 accaggcact gccgtccaga acaccgccgc tgtttgtgcg ctgagccgtg cggccggggc
     1861 aaacggtatg gtgtggggtc aggagcttga ccttgctttt gaagaaaagc ctgccacggc
     1921 tgaagaattg cgcgaggtgc accgcaacaa gaccggaatg ctcatcaacg cggcggcgca
     1981 gctgggagcg gtggcggcaa gtgcggacga tattcagcgg caggccattg cgcgatatgc
     2041 cttttccgta ggactggtgt tccagatcat tgacgatatt ttggacgtta cggcgacggc
     2101 gcagcagttg ggcaaaccca ttggcagcga cgcggccaat gggaaaacga cgtttgttac
     2161 attgcacggc gtggaacgct cccgcgggat tgcgcaggaa ttgacagacg gggcctgtgg
     2221 cgtcctacgc gcggcattcg gcggccgggc gcagtttctg gagaccttag cggccagcct
     2281 gctggaacga aatcagtaga aaggaccaga tcgtaatgca tgttttgcaa cggctttttt
     2341 tcgggaatta catcctgtcc gtcgctctgc tctcctggct ggcggcgcag atatgtaaga
     2401 cgatcatcaa ctatatcctg agcggcaaat ttgaagcgga gcgcatgtgg ggagcaggcg
     2461 gcatgcccag tgcacactcg gcgctcgtct gttccatgtt tatggccgca gcaaaaagcc
     2521 agggcgtcaa ctcgcccatt ttcgctattg catttatcct ggccgctatc gtaatgtacg
     2581 acgccatggg tgtgcggcgt gagaccggcg aacaggccaa ggtactcaac cgtatgatat
     2641 ccgactggct gagcgaagac gaggacgcac ctgcctatct ggcgcagaat ggccgcaagc
     2701 tgaaggaaaa agtggggcac actccatttg aagtgctttc cggcgcgtta ttgggaattt
     2761 tgatcgctgt gatcgtcccc gcaccctgaa ggcagtaaaa cgattgatag aaaggacgtg
     2821 aacggcacgc gccttttaga cacgatccat tccccggccg acgtcaaacg gctggatttc
     2881 ggacagctgc aggagctgtg cgcggagatc cgcgcgtttt tgatcgactc tgtctccaaa
     2941 accggagggc atctttcctc caatctcggt accattgaac tgacggttgc gctgcataag
     3001 gtgtttgcta cacccagcga taaatttgtc tgggacgtgg gacaccagtg ttatacgcac
     3061 aagatcctta ccgggcgcag ggaaggattc gaccggcttc gcatgctggg aggcatctcc
     3121 gggttcccaa gcccgcggga aagcgaacac gacgctttta tcgcgggaca tggcaatacc
     3181 tcactctccg ctgccatcgg catggcccag gcgaagaagc ttcggcatga gccgggcaag
     3241 gtcattgccg tcgtgggaga cggcgctttt accggtggca tgatctatga gggcatgaac
     3301 aatatccgta gcctgaacaa tttgatcgtc gtgctcaacg acaacaaaat gtccatttcc
     3361 aaaaacgtcg gttcggtggc gcagtatctt acccaactgc gcaccagccc gcgttatttc
     3421 aaagcaaaac gggatgtgga atctctcctg gactccacgc cggtcgtggg cggcgccatc
     3481 aaggcaggca tccaaacggt gaaatctgca tttcggcgtt cgttgtatca ttccacaatg
     3541 tttgaggaaa tggggttcca atacgtcggc ccggttgacg ggcacgacgt caaagaactt
     3601 tccatgttgt ttgatgcata ccggcaggag caaagcgcgc cgctgttcgt tcacgttttg
     3661 accataaagg ggaaaggctt tacgccggcg gaacaaaatc ccggcgaatt tcacggcgtt
     3721 tccgctttcg atgtaaacca tctgacggat ccggacgtcg cgccggacag ctctttttcc
     3781 acagtgttcg ggcagcagtt ggcacagctc gccgggcagg acgaacgtat ctgcgcgatc
     3841 acggcggcca tgaagtacgg cacagggctt cagtattttt ataagcagca caaatcgcgg
     3901 ttcttcgatg tgggcatggc ggaacagcac gccgtcacgt ttgccgccgg cctggcggcg
     3961 aatggaatgc ggcccgttgt ctcaatctat tccacgttcc tgcagcgcgg ttacgaccag
     4021 atcattcacg acgtcaattt gcagcgtctc agcgtgcttt tcgcggtaga ccgcgccggc
     4081 cttgtgcccg gtgacggcga gacgcatcag gg
//
LOCUS       DAWBSB010000203         4088 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_3719_length_4088_cov_2.582941, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000203 DAWBSB010000000
VERSION     DAWBSB010000203.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 4088)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4088)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4088
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3719_length_4088_cov_2.582941"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2529..2538
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3822..3831
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          958 a         1189 c         1157 g          764 t
ORIGIN      
        1 ccccggacat aagcctcgcc cgcattcgca aggagctggc gctcccggaa acggtgcaat
       61 tcaacggcgt gcgctgccgg gcaaacgaaa cgcttgaaga gagcgaaaaa aaggaaaagt
      121 tcgcctgcct gcccgcatac cacaaaatgc gcaaggcgct ggacggcgtt gtaaagggcc
      181 gtatttcctc cctgagcatt tcccaacgcg acgcggccgc caccgcgctt tccctttaca
      241 aaaacgagga cacgctgcgc gcaaagctga cggaggccgg tttccaagca cccgagatcg
      301 acgcgctggc gggccttacg ggcttttcaa aattcggcca cctttcactg aaggcctgca
      361 gaaaattgat cccgcatctg gagcaggggc tgacctacga ccaggcctgc agtgccgccg
      421 ggtacgattt caagggccac ggcgcaggtg agcgggcctt tacattgcct gccgccgccc
      481 cggaaatgga gcagatcaca agccctgtgg tacgccgcgc cgttgcgcag acgatcaaag
      541 tggtgaacgg catcatccgc gagatggacg caagccccgc atgggtgcgc attgagcttg
      601 cgcgcgagct gagcaaaact ttcggcgaac ggcaggagat ggaccgctcc atgcgcgaaa
      661 acgccgcgca gaacgaacgc ctgatgcagg agctgcgcga tacgttccac ctgctctccc
      721 ccaccgggca ggaccttgtg aaataccgcc tctggaaaga gcaggacggc gtgtgcgcct
      781 attcgctgca gcgtctggac gtggaacggc tgtttgagcc gggctatgtg gacgtggacc
      841 acattgtacc gtacagcctc agctttgacg accggcgcag caacaaggtg ctggtgcttt
      901 cctccgaaaa ccggcaaaag ggcaaccgcc tgccgctgca ttacctgcag ggcaaacggc
      961 gcgaagactt tatcgtctgg acaaacagca gtgtgcacga ctaccgcaag cgccagaacc
     1021 ttttgcgcga aaagctctcc ggggacgaag ccgagggctt ccggcagcgc aatctgcagg
     1081 acacgcagca catggcgcgc tttttgtaca actacatttc cgaccatctt gcatttgcgc
     1141 agtccgaagc gcttggcaaa aagcgcgttt tcgccgtcag cggtgcggta acgtcgcatc
     1201 tgcgcaagcg ctgggggctt tccaaggtgc gcgcggacgg cgatttgcac cacgcgctgg
     1261 acgccgccgt catcgcctgc acaacagacg gcatgatccg ccggatctcc ggctattacg
     1321 ggcacattga gggcgaatat cttcaggacg ccgacggcgc cggttcgcag cacgcccgca
     1381 caaaggagcg ctttcccgcg ccctggccgc gcttccgcga cgagctgatc gtacggctct
     1441 ccgagcagcc cgccgaaccg cttctggaca tcaacccgga attttactgc gaatatggca
     1501 cagagcatat ccgcccggtg tttgtttccc gcatgccgcg ccgcaaggtg accggcccgg
     1561 gccacaagga aaccatcaag ggcgccgccg cggcagacga aggcctcttg acggtgcgca
     1621 aggccctgac agagctgaaa ctggacaaag acggagaaat caaagactac tatatgccct
     1681 ccagcgatac gctgctgtac gaagcgctca aggcgcagct gcgccgcttt ggcggcgacg
     1741 gcaagaaggc ctttgcggaa ccgttttaca agccgaaagc ggacggcacg cccggcccgc
     1801 tggtgcgcaa ggtgaagaca accgaaaaag ccacgctcac cgtccccgtg cacggcggcg
     1861 cggccagcaa cgacacgatg gtgcgcgtcg atgtgttcct tgtacccggg gacgggtatt
     1921 actgggtgcc ggtgtatgta gcggacacgt taaagccgga gctgccgaac cgggcggtgg
     1981 tcgctttcaa accgtacagc gaatggaaag aaatgcgtga ggaggatttt gtcttttcgc
     2041 tgtatccaaa tgaccttgtg tacgcggagc ataaaagcgg gctgaagttt acggtgcaga
     2101 acgcggagag cacactggag aaaacgtggg tttccaaggc ttcttttgct tactttgtgg
     2161 gcggagatat ttccactgct gctatctcgc ttcgcacgca cgacaacgcc tatggtctgc
     2221 gcgggttggg tattaaaacg cttaaggttt taaaaaaata tcaggtggac gttcttggca
     2281 atatctcccc cgttcacagg gaaacgcggc aaagattccg ctgaacgatg aaaggaggca
     2341 gctatggcct accgatgtgt gatcgtcagc aaccccgcgc acatcagcac gcgcagcgaa
     2401 cagcttgttg tggaaacgga cgagcgccac accgtgccca tcgaggacat cagcgcgctg
     2461 atgctggaaa gccgccgcgc cacgctttca gcggccgcgc tctcggcttt ggcgcaaaac
     2521 ggcacggcnn nnnnnnnngc tgccatacgc gcagcacagc cgccagcttg ccgcggcacg
     2581 cgcgcagctt tcgctgacgc tgcccgccaa aaaacgcttc tggcagcagc tcgtcaccgc
     2641 caaaatcgac aaccaggcgg aatgtctggc gctgtgcggc aaaacacaag aagcggcctt
     2701 tctgcattcc cgcgcacgcg ccgtgacaag cggcgataag gacaatctgg aggcagcagc
     2761 cgccgcgtat tacttccccg ccctgttcgg cacaggcttc acccgcagcg ccgatgatgg
     2821 ccgcaacgcc gcgctcaatt acggctatgc cattctgcgc ggctatgccg cccgctgcgc
     2881 cgcagtttac gggcttttgc cgtgggaggg gctgcaccat tgcagccagc tgaaccaata
     2941 caatctggcg gacgacctta tggagccgtt ccgcccggtt gtggatttgt atgtagccgc
     3001 caatgtggac gaggcaagca cgctcgcccc cgcgctcaag cacgcgctgt tcgggctgat
     3061 gaacgctgac attctttccg gcggacagca ccacagcgtg gcttacgcca tggaacggct
     3121 ggtgcaaagc ctgcgcacca gcatggaaaa gggcggcgct ttggcgctgc cgaagctgct
     3181 ggcatggcag ccccacggtt atgagtagat ttatgagaat gcttgtattt tttgacctgc
     3241 ccgtccaaac aaaaaagcag cggcgggaag ccactgcttt tcgcaacttt ttgataaagg
     3301 atgggtatca catgcttcaa tactcggttt acgcccgcgt atgcaacggc aacgatgccg
     3361 tgacaaaaca ccgggcgcgg ctgacagggc agctgccggc caatggcgct gtgcgcctgt
     3421 tggtggtaac ggaaaagcag tatcagtcga ttgagatatt gctcggccca ttttccccgg
     3481 cggacacacc ctttgcatgt gaacagctta cgctgttttg atttttcgga aagaacaaaa
     3541 agcgcttcag gccgcagcct gaagcgcttt ttgcaacagt ctattatacc ataccaagaa
     3601 ctgttaggga actacaacct ctgatgcatc ttctaagtaa aaagcaaaat tataccatac
     3661 caagaactgt tagggaacta caaccgtgaa aacgttcctc aaggtgacga gcgcattata
     3721 ccataccaag aactgttagg gaactacaac gcaaaagacg cccggaagtg gatttgtgcc
     3781 attataccat accaagaact gttagggaac tacaacagag cnnnnnnnnn ncttcttgct
     3841 tgtacacaat tttgtctgga ttataccata ccaagaactg ttagggaact acaactattg
     3901 ccttcgccat gctctctgcg cgtaattata ccataccaag aactgttagg gaactacaac
     3961 ggacggcgga ggaaatgcgg aagaaatacg attataccat accaagaact gttagggaac
     4021 tacaacctgt tgaatccgcg cacaatggtg cgaaatatta taccatacca agaactgtta
     4081 gggaacta
//
LOCUS       DAWBSB010000204         4072 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_3741_length_4072_cov_3.474235, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000204 DAWBSB010000000
VERSION     DAWBSB010000204.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3741_length_4072_cov_3.474235"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2572..2581
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          712 a         1287 c         1375 g          688 t
ORIGIN      
        1 ccggctttga attttgaaaa ggcggtgttt ttgtgcctac gacggacttc ttttccggcc
       61 cgggctttga gcctgaggat gtaccggtga aaaaaacgtc tcccgcgcag cagccggttg
      121 aaaaacagcc tgcggacgat gaaccgttcg cgggaatgca gaagctggat ttcgatttcc
      181 tgggcggaga tgatcccgcc gtcccgggag agacggaccc cggagaccct ttcgacgatt
      241 tttccttcca gcgcccggcg gctccggcgg gcccggcgca gcccgcccgg cccgcgccct
      301 tcgtgttcga tgaagagcct gcccccgcgc cgttcagcgg cgtgccggaa acgcgcatta
      361 ccccggcggc gtccgcttcc ctcggaggga cggatgatgc acccgttcgg ccggtctctc
      421 cggcgcaggc tgcgcccaag gcggctccgt tcgtgttcga ttttgacgat gcgcctgtgg
      481 agcatcccgc atcccggacg gcggcgtccg cagctccttc cgaagaggcc gtgtttgatt
      541 tcggcattcc ggaagaaaag cccgcgccgg ctcctgccgc cgcaccgctg gacggctacg
      601 cgccgctgtc tctggatttt ctggacgatg cggaacccat tgctgctgtg ccgcccgcgg
      661 ccccggcggc tgtaaagcct gtggagccgc cgcgccctgc ccagcccgcg cccaaggcgg
      721 ctccgttcgt gttcgatttc gacgatgcgc ctgtggagcg tcccgcatcc cggacggcgg
      781 cgtctgcagt tccttccgga gaggccgcgt tcgatttcgg aattctggaa gaaaagcccg
      841 cccggcccgc gccggcttct gctgccgcgc cactggatgg ctacacgccg ctgccgctgg
      901 atattctgga cgatgcaaag cctgtcgccg ctgtgccgcc cgcggctccg gcggcggtaa
      961 agcctgcgga gccgccgcgc tccgcggagc ctgtgcccaa ggcggcgcct tttgtgtttg
     1021 atttcgacga tgcgcctgtg gaacgccctg cgtcccggcc tgggcagccg gggtccacgg
     1081 ccagtccctt gtattccgcg gcggatgcgc cgacggtggc gtttacgcct gccgcgctgc
     1141 aggcggacgc tcccacgacg gcgttcacac cggtgaagcc ggatgcgccc gcgcctgccg
     1201 cggccccgca gcccgcgccg cccaaaatgg cggcccagcc ggcgcccgcg gcccgtgccg
     1261 cgcgtatggc aaaggaaaaa gacatcggtg cggctgtatt cagcgctttg gtggcggatg
     1321 atgacgtgcc gcccgcgccg gccgttccgc ctgcatccgg cggggaggag ctgcgtccct
     1381 cggggtggcc ggcggcgacg ccgcgccctg caaacgcccc gcgcacgcca cggcctatca
     1441 actggcatgc gcccgtatct ccggcccagc gtcctgcacc tgcgcctgcc cggcctgcac
     1501 cgccgccggc gcaggcgccg cagcccccgg cagcggagac gccgttggat ttcgattttg
     1561 agggcgcgca gccctttgac ctgttcgacg atgttccgca gagcggatac accgccccgc
     1621 aggaaacgga ccaggcattc ccggacctgt atgaccggga cccggaggag gcagagcctg
     1681 cgccccgcgc tccgcagcgg cgtacccgga cgggcggcgg ttcaggctcc ggcggcggcc
     1741 gtccgccacg caggagtacc ggcacggccc ggcgcggaat accgaacaaa acgatttttg
     1801 cctgggcggg catcgtgctc atcgcgctgc tcatcatcct ttactttgtg ttctttggcg
     1861 ggaaggacgg agacgtggcg cccgggacct ctggctccgt accgtccagc acatcccagc
     1921 cggcagacag cacgccgccg gacgcatcct ccgcggcccc ggtggaaacc atcccgcggg
     1981 acgagtggta tatggtgctg gccaaccgca gcagcgtgct gcccggtgat ttcacggtgg
     2041 ccgagacggc gaccgtggga gaggctgtag tagacgcgcg catcgcggag gctctgcggc
     2101 agatggtgaa cgatgcggcc gccaccggcg tcaaactgaa gcccaccaac ggctaccgca
     2161 gcatcgcgcg ccagcaggag ctttgggacg cccgtgtcaa aaccctgatg gagggcggcc
     2221 tttcccaagc ggatgcagag acaaaggcca tcgactacac ctctgcgccc ggtacgtcgg
     2281 accacaatac gggcctgggc ttggacatcg tttcggaaga ccacccggca aaggacgcgg
     2341 gcttcgcgga gacagcagcg gcccagtggc tggccgagca tgcggcggat tacggcttta
     2401 ttctgcgtta tccgtcggac aagaccgagg cgacaggcat ggattacgag ccctggcatt
     2461 accgttatgt tggttccgag caggcgcata aaatcaagga aagcggcttg tgccttgagg
     2521 aatatctggc acagtaaagc agaaacggaa acgttgtggc ccgccccgcg gnnnnnnnnn
     2581 ngcggaaatg cgtggcgggc tttttgtgcc tgcacagggt atgaataaaa tgtaaaatcc
     2641 gcggcggcgg cgcggcgcgg ggttgaaatc gtgcccgcgt gtgttatact gaaaaagttc
     2701 cgaccgaccg gtcggtcgga attcgtccct gccggggagc aggaggagaa tccgcgtccg
     2761 cgcgtatttt ggagggaaga tatgcttccg caattcagtg taagaaaacc ctataccgtg
     2821 ctggtggcgg tagtgctggt gcttgtgctg ggcgttatct cgtttacggg catgacgaca
     2881 gaccttttgc cggccatgga gctgccgtat gtcgtggtaa tgacaacgta tcccggcgca
     2941 agcccggaga aaatcgaaac aacggtgaca aagccgttgg aagcggtgct gggtacgacg
     3001 ggcggcatta agaacgtcag ctcggtttcc agcgaaaacg ccagcatcgt tatcctggaa
     3061 tttgaacagg gaacgaacat ggattccgct atgatcgagc tttcgtccaa tgtggacctc
     3121 gtttcgggcc agatggacga tgcagtgggg acccccatgc tgatgaagat cagccctgat
     3181 atgctgcccg tgatggtggc cagcgtggat atggacggca tggacgtgaa ggagatatcc
     3241 gcctttgcgg acgaaaccgt tatgccggcc tttgagcgca tcgacggcgt tgcgagcgta
     3301 gatgccacag gccttgtgga gcagcaggtg accgtaacgc tcgaccaggt gaagatcgac
     3361 gcgctgaacg ataaggtcct tgccggcgtg gattccggtc tggcggacgc acagcgcgag
     3421 ctgcgtgagg gacaggccaa ggtggcggac ggcaaggcga agctggccga gggcgaagcc
     3481 gcgctggaaa gccaaaaagg ctctgcgctg gacaagctgg cccagggcag cgtccaggtg
     3541 gacggcgcct ccgccacgct ttccgcgctg ctgagcgagg agaccacgct tacagccaac
     3601 cagaaggcgt tcgaggccga aagagacggc tatacccagg caaagcaggg gtatgaaggc
     3661 atcaatacgg ctctggcggg ggcgcgggac accgctcgtc aggctgctgc ggctgctgcg
     3721 aagcagggag tgctggacag cgtgaacgcc ctgctggcac agatgggccg gcccgctgtg
     3781 ggcacttatg aggaagccgc cgccgtttac gcgcaactcc agcagactca gccgggcctg
     3841 cccgccctgc cggaccctgc ggtggtggga gaacaggccg ccgccgccgt tccagccaac
     3901 gtggacgcgc ttctggctct ggacgacgcg tcttttgaag ccttcaaggc gcaggcgggc
     3961 gcactgcccg gcggcgaaca gcttgccgcg ctgacaaagg aaagcctgac gcagctgcgc
     4021 gatgcggcgt tgagggcgga cacgcgtctg cccgaggtgg aggcggagct tt
//
LOCUS       DAWBSB010000205         4029 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_3791_length_4029_cov_2.949170, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000205 DAWBSB010000000
VERSION     DAWBSB010000205.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4029)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4029
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3791_length_4029_cov_2.949170"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          859 a         1304 c         1105 g          761 t
ORIGIN      
        1 cgtgtgccgc tttttttctt ccagagccgg aaaccagcca taagagccaa cccgaacggc
       61 agcgccagca gacccggcag ctcgaaatgc accagcagac ccgccgccac ataagagatg
      121 caggaaacgg ctgccgccgt tattgcatag ggcagttgtg tggagacatg ctggacatgg
      181 tcgcactggg cgcccgcaga ggccatgatc gttgtgtcgg agatcggcga acaatggtcg
      241 ccgcacacgg cgcccgccat acatgcggca atgcacacga tgcccatggg gtcgtccaga
      301 gggaagatcg ccatggtgat ggggatgagt acgccgaacg ttccccagtt tgtccctgtc
      361 gcaaaagcca gcaggcagcc gatcaggaac acgatggccg gcaggaacat ctgcatgcct
      421 tccgcgccgc ttttcacaag gtcccgcaca aagtatttcg cgcccagaga atccgtcatg
      481 gctttcagcg accatgcgaa tgtgaggatc ataatggcag gcaccatcgc cttaaagccg
      541 tccgggatgc agcccatcat ctcgcggaag gacattgccc ggcgcagcat ataataaacg
      601 aagctgaaca ccagagcgaa tgcggaccca agcatcagcc ctaccgacgc gtcggagttt
      661 gagaacgcgg tgctgaaacg cgccagcacg ccgccttccc cgcctgcgaa gaagccgccg
      721 gaatacacca tgcccaccac gcagcagaaa ataagcactg caatgggtgc aataaggtca
      781 accacacgcc ctttgtccgc cggggcctcc cctgcgcccg cataggggtc cggcccgccg
      841 ccgaacaggt ctcccccgcc ggccagcgcc tcgcttttcg ccatggggcc gtaatccacc
      901 cgcagcacaa caagcatcac catcatggcg atggtgaaca gtgcatagaa attataggga
      961 atggcccgga tgaagaggga caggcccttt ccatcctcca caaaaccgga cacagccgcc
     1021 gcccaggacg aaataggcgc aatgatgcac accggtgccg ccgtagcgtc gatgatatac
     1081 gccaatttgg cgcgcgatac ccggtgcttg tcggtgacgg gccgcatcac ggagcccacc
     1141 gtcagacagt tgaaataatc gtcgatgaaa ataaggcagc ccagcgctac cgtagccagc
     1201 tgcgcgccca cgcgggattt gatatgtgtc tgtgcccatc gtccgaaggc cgcagaaccg
     1261 ccggccttgt tcatcatgca gaccatcgtg cccaaaacga caagaaacac caggatgccg
     1321 acgttgtatg catccgacag caccgagacg atgcctccct gaaatacgtg cagcaccgtg
     1381 ccctcaaagc tgaaattcgc atacagcagg ccgcccgcag caatgcctat aaacagtgaa
     1441 gaatacacct cttttgtgat gagcgccagc gtgatggcaa tgagcggcgg cagcagggac
     1501 catgccgtat tgtaaaaccg gctttccgcc tctgtcctgc cggagccgcc gcagaccccg
     1561 cagatgtttt tcccgccgca cgccccgcat gccgcgtcct tgcccaggca ttccatgcaa
     1621 gcgcccgagc cgccgcattc cgcgcattcc accatgcgcg aacccggttc gtcctgcgcg
     1681 ccgctttccg caaaagccgg cgccgcgcac aaaaccgcca gcaccagcac cgccgccagc
     1741 atgggcagca gccgaatcct ttttttcata ctgttcctcc tcctgtgttc tgccgcaggc
     1801 cctccgctcc gacacacgga cacaggctgg aaaaaacaaa aagccctgtg cgcatagagc
     1861 gcacagggca accggacgca gcctccgctg gaagatgcgt ctggatagcg ctccacaaat
     1921 tttgtgacag tccgcaggat attctcccac ggcccaggat acgccgccca ggcaggcggc
     1981 tgctccttcg gcggttcccc ctctccccga aacggctgcc tggccattgt tccgggtacg
     2041 catgagtccc gcgcctctat ccgcgtattc agttgaaaag acctttccgg ccttcccgaa
     2101 gcacacaacg cgcccgggaa cattcgaatg atacttataa attagcacgg ctgctccaaa
     2161 atagcaagcg gatccgccat tttttacaca aaaaattttt ccggatgcac acggcctttc
     2221 cgcttcttca aaaaacgccc cggcatccgc cggggcaatg cgtttcgttt ttttcagggg
     2281 tctcaatcgt gaaggctggg tttcgcccgc agagaaatgg tgtgctcaaa acagctgtaa
     2341 tccgtcaaat acacagtata gcagccctcg gtgcgcttca tgcttccaaa cttacccgct
     2401 gtgaagccgt gcgcgccgaa gcacgggccc gcctcgatgg cataccttcc cctggaggcc
     2461 gtcagcgttc cggatttggc gacgataccg ccgccctccg cgccgtccgc ggcaaaatgc
     2521 tccgtctcca cgcggcagcc cgcgtcgaac gcaagctcca cacgcagagg cgcacggtcg
     2581 atcccggtgt tgacgatgtg cacgtcaatg ccgttctccg cctcggtgac ctccacatcg
     2641 aatatcatat ccggcccgtg aagctgcgcg cgcttcgtgt ggtccatttt ccaccagtcg
     2701 ctggtctccg gcttttcttc aaacggcaga taataccagc ccgtcatggt ctggtgcagc
     2761 gcataggcgt tttcgcccgt ggaggccagc gtctcgggga tgaagctgcg gtgctcacag
     2821 aaactggcgc cgatgtgcat actcacagta aaatcgccgt tctggaaata taaaaagccc
     2881 gcgctgtggt tgatgatggt gtaggaatac cccttccggc ggcagcgtac gatctcgctg
     2941 cccttgtaaa atttgtggta atccgccggg aaaccgctgc ccgcgtgctc cagcgcggcc
     3001 aggcggggct gcagcatgaa ctggatcagg caatccatag ccttcaggcc gtggcggtcc
     3061 actgccgcca tgatctcatt cgccgcgtcc agaaattccg gcttctgcag cacgtcgccc
     3121 atatacagat actcaaagta atagtctttg ggatagatct tgcgcccccg gtcctgccgg
     3181 gtggagttgt tcgtaaagat gctgtcgtcc ggctcgatat aggtgagcat catggtcagg
     3241 ttccgcacca caggttcgta ataagcgtcg tcgcccgtgg ccacggccag catgatcatc
     3301 gcgtcgttgt tgatccggtt gtaattgccc gccgagcgct ccgcatattc gccgtctgcg
     3361 ttgcagtcgc tgccttccag caaaatagcg tccgcggcct ttttgtacgc ctcgccgccc
     3421 agcagcctgt ggcacatcat cagtaccgat gcgatggccc agcggtggtt cggcgtatga
     3481 aaaccgcagt gcgtcagtgc ttcggcggca tctttcacaa ttgcttcata cagcggcagc
     3541 agctgcgcgc acacatcttt ttccgccgcg tccgccgcct gcgcgccctc cgcgatccgg
     3601 cgcagatata catacacggg cagcagccgc ttcgtgcaga aagccgtgtc cgggccggaa
     3661 aaaaagttgc agttgacgag gtcgaaatag ccgcccggcc gctgggagcg gcgcacataa
     3721 tccagcgcca gcaaaatgcg ccggagcacc tctgcgtcca gatgccattt gctgtccgcg
     3781 ttcaggcagc aggccgtcat ggtcgtcaga tgatagatgg ccaccttcgg ctccgcaatg
     3841 ctgtccgccg aggggaatcc gccaaaaaac gggtcgttcc ggtccaggat ctgaatctcc
     3901 ataaagcgtg cgacacgggc gtcgctgtcc cgcaggacct tgttgtaatg ctctttcata
     3961 tgggggctcc ctttccaaat agaagtgacg gcaaaagccg gtgcttgtgc gccgcgcggc
     4021 gtgccgccg
//
LOCUS       DAWBSB010000206         4024 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_3795_length_4024_cov_2.614261, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000206 DAWBSB010000000
VERSION     DAWBSB010000206.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 4024)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4024)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4024
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3795_length_4024_cov_2.614261"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1099..1108
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1045 a          999 c         1115 g          855 t
ORIGIN      
        1 ctattaaccc aacagtataa taggtttata tgtggaaatc taaaaaatag cttttggtgg
       61 gtcttggact atatgggcag tcctcggcca ttgagataag ttttttattg cagagaaata
      121 aaaaaaacag ggtttgaact tttgctatcg aaaagtttta aaacataaat acccttgcaa
      181 atactggttt ttgtgtatca agacacttaa aattacaatg agtttggcac tttggcgtcc
      241 ccggagttgc ccgaatttct tagggcaata aaaggcacca actttaaatt tatcgaggag
      301 gcgaacaatg attaaagtaa taaaaaaagc gtttgttgtc attgggaaag agggttcaac
      361 gctagacggc gcgggcttta ttcagaaatt gtgggatgat gcaaacggtc acttcagtga
      421 agtagcacat ctggcgaaga aagatacaaa cggaaatatt gtgggaatat ggggggccat
      481 gtccgatagt tctcacgcat ttaatgcctg ggaagatggc tttagcagag gcttgtatct
      541 ggcgggagtg gaatgtgttg ataatgcaga agcaccggct ggatggacaa aatggataat
      601 accaggttat gagtatatag ttgttgaaaa ccataatgga gcgtttgaag ataccattaa
      661 acaaatgaat gaggaaggta tgtcgctggt cggagcagtt cacgactata ccgatccagc
      721 aacgggaaag ggttatatgt attttccgat aagagaaatt taataaattt gtatttgtat
      781 actgtcatag atgaaaacga gtgattttta cgggaagggt gcccctttcc actgcttgct
      841 acgggatttt tcgcaaagga acagaaagga aatgataatc tcaaggcagg gtcttttctt
      901 tgatatattg cgcagctaaa tggcgttttc agggggacat gtataagcat tttaccccat
      961 cggttttatt gccggtactg tttttatggc aaggcttttg aacttcgaat gcgtaaccat
     1021 aatggtgtac aaagttatcc taaattgcct ttgcaggcgt gattgcatcc ctgtgcctgt
     1081 tctgtatcat cttagctgnn nnnnnnnnct acaaaaccaa cctatacggc gatgtgagaa
     1141 ggtacaagcg gaaatgggaa aacggttgaa aatgcaggca gtttccgcac ctcaataaaa
     1201 ctcgcaagat gttataagac aatttccgtc tatacaatcc ataaaaaata gccgcaggct
     1261 gtttttaggc tttgcgggac gtccccggcc cgcggtcagt aagataagct ttttattgcg
     1321 gagcaataaa aaggccgcgg gaaagggccc gcggcagagg aaacaaaaag aaacggagga
     1381 acggctgtat gatggtgtcc accaaggggc gctatgcgct gcgtgttatg atcgacctgg
     1441 ccgagcacca ggcagaggga tatatccctc tcaaagctat tgcccggcgt caggatatct
     1501 cggaaaaata tctggagagc attctcaaaa gtctggtgca gagccagttt ctgacaggcg
     1561 tgcgcggaaa gggcgggggc taccggctga caaggccgcc cgagcagtat accgtgggaa
     1621 gcattctgcg gcttacggag ggcagcctgg cccccgtggc ctgcctggaa caggagccgg
     1681 tggaatgtcc gcgcagggcc gaatgccgca cgctgcccat gtggcggaag ctgaacgcgc
     1741 tggtgcagga ttatctggac ggcgtcacgc tggcagatct gacggccgag gcccagtccg
     1801 ccgaccatta tgtgatttga cccggctccg ttcaggcata gcggtatttt cccctttttc
     1861 gccacagaaa ccaggcgctc accgccagag ccagaagttc ggccacaacg atggcgagcc
     1921 agatgccgtc cgtccccatg agagcgggca gcaccagaac cacggctgtc tggaacacca
     1981 gcgtgcgcag gaaccagata acggcggaaa caaagccgtc gttcagcgct gtaaagaagc
     2041 cggagctgaa aatgttgaag cctgtgagca ggaacgaaag cgaatacaaa cgaaagccgc
     2101 ggcaggtcat cgccagcagc gccgcatcat agcccacgaa tatccttgtc agcggaagag
     2161 ccagtgcctc ggcagctgca gtcagtgcca ctccggccgc gcccaccagc agcaggcttt
     2221 tgcggaacag gcttttcagc tccgtgctgt tccccgctcc atgatggtat cccaccacgg
     2281 gcgcgctgcc gatggaataa ccgatgaaaa tggcaatgaa aataaagttg acgtacatga
     2341 tgacgccata ggcggcaatg ccgtcctccc ccgccagctg catgagctga aaattgtaca
     2401 gcactgtcac cagtgaagaa gagaggttgg tcatcagctc cgacgagcca ttggtgcagg
     2461 ctttcagcaa tgcacggctg tccagcttcg tttttccaag ccgcaaaagg ctgcggttgg
     2521 gccgggcaaa atacagcagc ggtacgccgc cgcccacaaa ctggctggcg gccgtggcaa
     2581 tggcagcgcc cgccaagccc cagcggaaca cggcgatgaa caaaaagtcc agtatgatat
     2641 tggtaagccc cgcggccacg gtaacgccca ggccaaaatg gggcttttct gctgtgatga
     2701 gaaagctctg gaacacattt tgcagcatga acatcgtctg gcccgccagc aggattccgc
     2761 cgtaaacgac gcagtcctcc agcatggcac cctgtgcgcc caaaagcacg gcgatggggc
     2821 gcaggaacag cagccccagc acggaaaaaa cgatgcctcc gccgatggtc acatagacaa
     2881 gcatggaaaa ataacgctgg gcccggccgc tgtccccttc accaagggta cgggccacaa
     2941 gagcgctgcc gccggtgccg atcatgaagc caagcgcgcc caggatcatc agcagaggga
     3001 aaatcaggtt cacggccgca aaagccgttt tgcccacaaa attgctcaca aacaggccgt
     3061 ccacaacgcc gtaaacggag gtaaagatca tcatgacgat ggacggcgcc acaaagcgca
     3121 gcagctttcc cgtggtgaag tggtcggata attggatcct catagtatgc tactccttga
     3181 aacggttggg ataaatgtgc cgcgcgcatg tccgcgcggc agaaaaattg cgcccggatt
     3241 tccaaaaccg agcgcactcg tcatcttctc cggctgtatg gctgcggcca tacgccctcc
     3301 gatgggatat ggaaatttac aggatgcgtc cggcctctgc ccgcaggcgg gcaagatagg
     3361 cgtgcgtcag acgcagaagg gcctgctggt cctcctgcgg catgccggaa aacgcgcgtt
     3421 cctccatggc ggccacaggg gcggcttttt cttccaccag cctgcggccc tccggcgtca
     3481 ggcagatgtg catgtctctg ccccgtccgg gctgaagata cagatagcca tcctgttcca
     3541 gcctgcggat ggaggaattg atggtctgct tgctgatgta ggacagatcg cagatatccc
     3601 gctgcaggca gccgtcgccc agaagataaa gcgcatacag aatgatggag gcgctgtcgg
     3661 aaagcccctg ccgcagtgca agattgtggt aaagatcgtc cagctctttg taaagccggt
     3721 tgaattcccg tattccgggg ccctgtccca tatgcatgcc tcccatatcc gatttcggac
     3781 tcaatgcgtt tattatagtc cgatttcata cttaagtcaa ggctctcaag caaaaaggga
     3841 taaaaacagt cggcgcagct actatttttt acgcgcatcc gggcactttg tgcccgggcg
     3901 cctgagatct taaggccgtg ggacggaggg cccacatggc cgctttttat agcttgctgt
     3961 gcaaagctat aaaaaagcag ccggccagaa gattcggccg gctgttctgg cgcagcggga
     4021 ggga
//
LOCUS       DAWBSB010000207         3956 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_3853_length_3956_cov_3.015893, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000207 DAWBSB010000000
VERSION     DAWBSB010000207.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3956)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3853_length_3956_cov_3.015893"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          722 a         1180 c         1199 g          855 t
ORIGIN      
        1 cgactgccgg attccaattg gcttacaaac accgtggata cgccgatctt ctccgccagc
       61 ttttcctgcg tgagccccgc gtcccggcgt gcaagccgga tgcgcgtgcc gatctcccga
      121 ttgtccatct cgcaccgccc cgtcgtcatg ttttgatatt agcttacagc taatgttaat
      181 cttttgtaat aaactaacag ctaatttatt attgctataa gttaatatat gtgatacaat
      241 gagtttaacg cttcgtggga tacgtttgcc cccgcccttc cggtgcgcca ggaattccgg
      301 tacaaaaaaa cgccgcatcc gaagatgcgg cgttgtaaga aagcagctgc ttacgcggca
      361 gcggaagtgc tggcgctctc agaggtgctg gcggaaacag attccgcagc agactcggag
      421 gtgctggcag aggaagcgtc ggcggccgga gcaacatact cctcgccgtt gatcgcctcg
      481 gtgtagccgg cgatgcactc gaacatatac gcgtagtcgg cgcccgtggc cgggtcaaca
      541 gcgcgctgcc agctgtaaac gaagtcatgc gccgtcagcg gggtgccgtc ggaccatttc
      601 aggccttcgc gcagatggaa cgtataggtc aggccgtcct cgctgatctc aacgctctcc
      661 gcctggcccg gggtcggggt ggtgccgtag gcaaccgtgt acaggccttc aaaagcgtgg
      721 tcgatcatcg tgccgccgtc cacagcactg ttcagcgccg ggtcgatggt gtcggggtcc
      781 gggccggtgc aaaccttcag cgtatccgtg ccgttgctcg cgttcatgaa gaacttgaag
      841 cccagcgggg aggtgctcag gttttccagg ttctgctggg ccatataaat atccacatag
      901 tagtaaatcg ggcacagcat ccactcgtcg aagatgatgt cctcagcctg atgcatcatc
      961 tccatgcgcg cctcagcgtc gccggaggac ttgatctgct tgatcaggct gtcgaactcg
     1021 gcgttggacc actgggcgtc gttgttgccg ccgccggtga tccacatatc caggaagctg
     1081 atggggtcgt tgtagtcgcc cagccagccg ttgcgggcaa cctgatagtc gccgctctta
     1141 cgggtgttca ggaaggtggc ccactcctgg ctctccagcg tcactgtggc gcccagctgg
     1201 ccccacatct gctgcagcgc ctcacccacc tgctgatggc cggtgccttc gttgtacaga
     1261 taggtgatgg tgggcaggcc ctcgccgttg ggatagccgg cctcagccag cagctccttg
     1321 gccttggcca gattggcctc gttggcctcg gcggtcgggt cataatagtc gccgcccact
     1381 tcgcggaagg aagatccttc cgcagcatcg ctcaggccct cggacacgaa cgcgccggcc
     1441 gggatctggc cggatttgcc gatctgcctg cagatatagt cgcggtcaac cgccagcgtg
     1501 aacgcctggc ggaccagagg atcgtcaaac ggagcggcct gcgtgttgta gctcacatag
     1561 taggtgccga tctggccctc tttgtagaac tccggcgtgg aagacaacgt gtcgatctcg
     1621 tcgttgggga cagcgtcgat gaaatccagt tcgccggact ggaatgccgt cagctgggct
     1681 gcgtcatcct ccatcagcgt gaactgcagg gtgtccgggc cagccaggct gtccacgttc
     1741 cagtagttct cgttcttgac catcgtgatg tgggagctgg gcacccactc agcaatcttg
     1801 taggggccgt tgccgatgta ggtggcagcg tccacagccc aggcttcgcc gtttgcctca
     1861 acagtcgcct gctgaacggg agcatacgcg gggaacgccg tcagctccag gaaatacggc
     1921 gtgcgggccg tcagcgtgac ctgcagcgtg ttgtcgtcca gagctttgac ctgcagcgca
     1981 tccggattgt aagccggagc ggcgtcggaa gcagagccgg acgcggaaga gcccgcggga
     2041 gcggagctcg cagtaccggc ggacgaagaa gtgctgccgc cgcaggccgt caggcccagg
     2101 ctcagcacca tgacgacggc cagcacaagt gccagtttct tcatagtgaa ctttcctcct
     2161 caaaaattgt ttattcaaca tttcacacat ggcgcaccca tagcatgccc caaggtgaat
     2221 tgtttacaat tatacacgcg cggacgcgcc gcgcaacgct tttttgcaaa ttgtcacttt
     2281 tttgcaactt tattataata aagcacccgg gcggaaaaac ggcgctttca gcgccttttt
     2341 ccatttattt tactttgtcc agatggtggc aggcggcata gtgtccgccg gacacctcgc
     2401 gccatttggg ttcctctgcg gcacactgct cgtctgcgta ggggcagcgg gtgcggaagc
     2461 ggcagccgga aggcgggttc agcgggctgg gcacatcgcc ttccagcacg atgcgccggg
     2521 attcccgggc cgtcttgggg tccgccaccg gaattgcgga gatcaggctc cgggtgtagg
     2581 ggtgaacgct gtggaaggtc agctcatagc tgtcggccag ctccaccagc ttgcccaggt
     2641 acatcacgcc gatgcggtcc gaaatatgct tcacgaccga aaggtcgtgg gcgatgaaca
     2701 ggtacgtcag gccgtactgg tcctgcaggt cctcaaacat gttgacgacc tgtgcctgga
     2761 tggaaacgtc cagcgccgag atgggctcgt cgcagacgat aaactgcggg tccacggcca
     2821 aagcgcgggc gatgccgacg cgctggcgct ggccgccgga gaattcgtgc ggatagcggt
     2881 tggcatgctc ggagttcagg cccacacgct ccagcatttt aataatgcgc tcccggcggt
     2941 ccgccttgga ggacgcaagc ttgtggatgt cgatggcctc gcccacgatg tcgcccaccg
     3001 tcatgcgggg atccaggctg gcataggggt cctggaacac gatctgcatc ttgcggcggt
     3061 acggcagcat gttcacggcc gtctttttgc cgaatttcgg cttgccgctt tcgtccagcg
     3121 ccggggcgcc gtcctcgttg tacagcggca catttccgct gtcaaacagc gtttcgccgt
     3181 catagaggat acggcccttc gtcggctcgt acaggcgcag cagcgtgcgg cccgtggtgg
     3241 tttttccgca gccggattcg cccaccagcc ccagtgtctc gcccttgttg atggcgaatg
     3301 aaacatcgtc cacggcctga acgtactttt tgcttttgcc catgccgccg gcggggaaat
     3361 attgctggag gtgctgtacc tctaccagtt tttcactcat tgtacattcc ctccctgtgc
     3421 ggcttcaaac tccgcctttt ggcagagcca gcactgcgtg tagtgcgtgt cgctgaaatc
     3481 ggtgcgcggc ggcatctcgc gcaggcacac cttcatgcac gcgccgcagc gcggcgcgaa
     3541 cggacagccc ttgggcgggt tgagcatgtc cacgggcgtg ccctcaatgg gcaccaggcg
     3601 ctcgtgatct ctggaatcca ggcgcgggat actgcgcagc aggccctttg tatactcatg
     3661 cttgggttcg tagaaaattt cgtccgtggt gccgtattcg acaatcttac ccgcatacat
     3721 gacggcgatc ttttcgcaca tgcgcgccac gatgcccagg tcatgggtga tcatgatgat
     3781 ggccatgccc agcttttcct tcaggtccat catcagctct aaaatctgag cctgtatggt
     3841 cacgtccagg gcggtggtcg gctcgtctgc gatgagcagc ttgggctcgc atgccagcgc
     3901 aatggcgatc atcacgcgct ggcgcatacc gccggacagc tcgtgggggt actgct
//
LOCUS       DAWBSB010000208         3918 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_3911_length_3918_cov_2.559151, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000208 DAWBSB010000000
VERSION     DAWBSB010000208.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3918)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3918
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3911_length_3918_cov_2.559151"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2966..2975
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          845 a         1093 c         1059 g          911 t
ORIGIN      
        1 cgttactttc acgtccttac aaatcgttct gtttccgagc gcttatttca gcgccgcgcg
       61 cagctgatcg ctggcggctt tcacgccgtc gatccaattc tgctcggtct tgtcgcccgt
      121 cacaaggctg ttgacggggt cgaaccagac gctgcgggta tcgaggccct cgatggcctc
      181 agtggcggca aagctgccca gtgcggcctt cgcgccgttg tcataaatgg aatagaacat
      241 cttgttgtcg ccctccagcg tttccgccag gcccacgatg ggctggatgg cgcccgcctt
      301 ggcaaagatg gccgcggctt cgtcgctgta caggtaggcg atgaactgct tggcaaggtc
      361 ctgatgctcg gctgccgccg ggatccaggc ctgctcgaac caggtgtagc tgtaacggtc
      421 gccgccctct ttcacagcgg gcagggccgt gaagccccat ttaaagccat cggcgcgggg
      481 cgcttccgcc atctcgccca cgatccaggt gccgttgggc ataaagatgg ccttattgtc
      541 cagcacaagc tgctggttct ttgtaaagtt gtcgtcgttg gcattggccg gggtggtagg
      601 ctcggtgtac tcggccagct tggcaacaat gtcgaagcag gtcttggcct cctcggtgtc
      661 ccagatgcct tcttcataac gcgtggcctt gtcgaagaac tcggggccgc ccgccgtgta
      721 catcagagcg tagaagaaag cgtcgaagta accggtggtg gggtaggtaa acaggctgat
      781 gccttcggcc ttcgccttgt cgcccagctc ccacatctcg tcccaggtgg cgggcacttc
      841 ccagcccttt tcttccagca ggccggcgtt gtagaacagg ccgcaggggc tgtagaacat
      901 gggcgccaga tacgttttgc cgtcgccata cggattggtc agggaggaat ccgtgaagcc
      961 acccgcaatt ttatccttca ccttgacgtc ctcaccgggc accgtcatgt ccagcacatc
     1021 ggtaatgtcc agaatgccgt tgtctttaat gaacgtttcg gtcaaagccg cctcgcggcc
     1081 cgtggccaga tggatgacgt cgggataatc gccggccttc atactcggac cgataacatc
     1141 ctccagcttc ttgtcggtcg tcagttcaac cgtaacgccg gggttggcag cctcaaacgc
     1201 cgcgcacact tctttccaca tgtcgctgcc ataggccgtc tcgatggcgg ccaccttcag
     1261 cgtctgctca gcgggcgccg aagagctgcc ggccgtggag ccggagcttg ccgccggcgt
     1321 cgaagacgag ccggagccgc cgcagccggc gagcagggtg agcgccatgg tgagcaccag
     1381 cataatgcta agtagctttt tcatagttta cctcctgaaa atcttgttcg cgtttgtttg
     1441 cgccgggcct tgcgcccggt gcatccgtcc cctttagtat atcttttccc ttttcgcatt
     1501 caacaatatt cttgctccat tttttattta tcttgtcttt ttatcatctg cgatattaat
     1561 tttgacttac ttttgtaatt tttatgcaca gtatccacaa aacaagcttt ttgtgtttgt
     1621 tcacctttcc aaatgtgttc aattgcagat tattttttca caaacagcgc tttatggctt
     1681 tcgcgcaaaa tatttgcgcc cggttcccaa aagtattata atggttaacg gcagcaactt
     1741 tgcttaaaca aaagcaacat tcataaaaca atcttgcgaa aatttatact tggccgtttc
     1801 tgcatgcggc gcacgaaaga aagttgggaa aaccgtcatg agcaacaaac gatacgaggt
     1861 aaacaccccc ggcgtcagcc ctgtggaccg ggacgtcacc aaactgctgt acgtgagcac
     1921 cgccaaatac gggggcgact ggcacagcct gatgcatacc catgcctgca ccgagctttt
     1981 ctatgtggtt ggcggcatgg ggcagttcaa agtggcggaa aagctgattc ccgtctctcc
     2041 ggacgacctt gtcatcgtca accccaatgt ggagcacacg gaggtaagcc tgaacgccag
     2101 cccgctggaa tatatcgtgc tgggcgtgga gggcatggaa ttcgccgcgg acgaaaacgg
     2161 tgacaggcgg tacagcgcat tcaacttcca cagcggacgc gaggatattc tgccctatct
     2221 gcggggcatg ctgcgggaga tcgagcagaa atccgcaggt tacgaagtcg tctgccagga
     2281 tcttctggag gttctggtcg ttaagctgat gcgccacacg gatttttccc tcacggtcac
     2341 gcccgcccag cagtccagca aggaatgcgc tgaggcccgg cggtacatcg acagcaattt
     2401 taaagaaaat atttctctgg accagctggc ggaactgacg catgtgaaca aatactatct
     2461 tgtccactcc ttcagcaaag aatacggcgt gtcgcccatc aattatctga tcggacggcg
     2521 catcgaggag agccgctacc tgctggcgga caccaatcac tccctgtccc agatctccca
     2581 catgctgggt ttttcctccc ccagttactt ctcccagagt ttccgtaagc tggaaggcat
     2641 tagccctatg gaataccgga agcggcagcg ggccgcgcgg gagggacatc cggaataata
     2701 acagttgtac ttttgttcat ttatctttac acaatcgtcc gtatatgctt taatagatta
     2761 taatgtaaca gtctgacaga aaggcggaat actggcatgg gctacgaggc catacggctg
     2821 cagcggccgc tggcggtgga cgatatcgtc tccgtgcatt attttgaata ttccagcaat
     2881 tactattttg aaggggagcg ccacgatttc tgggaatttt tatatgtgga caagggcgag
     2941 ctgcatgtgc gtgcgggcag ccgcgnnnnn nnnnnggacg tggtgcagca tttgaagaaa
     3001 gggcagatca ttttccataa accgggcgag ttccacgcgc tctcggccaa cggtgtggtg
     3061 gcgcccaatc ttgttgtggt gggttttgtc tgccacggcg aggccatgcg tttttttgaa
     3121 aacagagtat tgagcgtggg cgattccggc cgcgccctgc tggcgcgcat cgtggaggag
     3181 ggcgaggccg cattctccac gccgctcaac gacccgcaga ccatgcagct ggcccggcgg
     3241 gagggtgcgc ccttcggcgc cgagcagctc atcggaagca gcatcgagca gctgctcatc
     3301 ggcttcatcc gccgcggcgc ggacgaggcc gcggccgccg caaagcccac cagcctcatc
     3361 cgggaacgca cacagcagga attcatcgac cgtgtgagca aatatctgga ggacaacatc
     3421 gcaaaacgcc tgacgctctc ggatatctgc cgggacaatc tggtgggccg cagctatctg
     3481 caaaagatct ttcgggaaaa gacgggcggc ggcgcaatgg aatacttcgg cacgctgaag
     3541 atcgacgcgg ccaagcgcat gatccgggag ggcacgcaca atttcaccga gatcgccgcg
     3601 ctgctgggct ataactccat ccactatttc tcccgccatt tcaaaaaggt tacgggcatg
     3661 acgcccagcg aatacgcctc cagcgtcaaa attctggcca ccaaaaaccg tgttgatacg
     3721 tccagctttt tagagacttc taaaactgct aattactttc cagatgagcc ttttcagctt
     3781 cctttcacca accacttcgg agttcagttt cgaaacgctt cctgcgaatt agcaatttct
     3841 agagagactt atgctgaaat agataataaa agttctttca ctaaaggaaa aatatattct
     3901 gatgactatt gtcaaaaa
//
LOCUS       DAWBSB010000209         3893 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_3942_length_3893_cov_3.182387, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000209 DAWBSB010000000
VERSION     DAWBSB010000209.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 3893)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3893)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3893
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3942_length_3893_cov_3.182387"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          694 a         1164 c         1233 g          802 t
ORIGIN      
        1 gtccagcatg tccagcatct gtccgtcccg gttggagacg ggcagcatca gcagcagcgt
       61 gccgcacagg ataacggcaa gaaagctgcc gacgatcaac cgggtagggt tggtgttttt
      121 gcgccgggcg ttgtgggaaa gcgcctcggc cagagaaaac cccttcaaca gaacacctca
      181 ttttgcgtgt ttgcatagaa taataggtta ttaacaggaa ccgcctgttt ttttgcatgc
      241 tattaatata cactacctcc ggaccaaata caaccgccaa aggcggagga aatgaacaaa
      301 agccgatttc gaagcgcggg cttgcaattt ttttaccgtg cctttataat ggaatagctg
      361 cagaaatgcg gcggttttgt ctccgtagct cagttggata gagcgaacgc ctcctaagcg
      421 ttaggccgct ggttcgattc cagtcgggga caccatgccg gatacggttg tccgtgcgga
      481 aagccggagg gtgctttgaa tgcggcctcc ggctttgctg tatcctttgg aggcatgcat
      541 cctgtacatc gcccggcggc ggggggcaag gtggcattgc ccgatgacgc cgtctcctgt
      601 cattgcgaca acctggcccg ccgccttgta tgcgcgcacg atgcgaagct tgtgggcggg
      661 ggaaacgcgg gcgtaaacag agacgcgcat gcaccgcgcg gcaagctccg aatcggacag
      721 cgcgtccagc tccgtgcccg agagcacgcc gtcccccgcg cgggcgatgc ctacgtcccg
      781 ggcaatggcc agcgctgttt ccttgtaatc tccggtgatc atcacaggct tgatgccggc
      841 ctcgcggcac cgctgtacgg cggggcgcac ctcgggccgg ggcgggtcca gcatgcccat
      901 caggcccaag aagcacagct tttcctcccc ggcggcgggg gtgctgcttt ccgcgaagcc
      961 cagcacgcgc agcgcttcgc ccgccatgcg ggcacaggcg gcgttgatat cggcgcggtc
     1021 ccgcggggcc agcagccgcg gacctgcggg ggtttcgata tgcgtacagc gggccagcag
     1081 catatcgggc gcgcccttgc acagcaggcg tacgccgccg ggaccgctca cggtcacgct
     1141 catgcgtttg cgtgcggcgt cgaatgggtt ttcgcctatg acggtatatt gctgcagcag
     1201 cgcctctttt gttacgccgg ccttggcggc ggccaccagc acggcaacct cggtcgggtc
     1261 gcccatcacg tcgtagacgt ctttggcgcg ggtgatgtgc gcggtggtgc acagggaggc
     1321 ggcctcgcac agcctcccca gtgccgcgtc ccgcgcggcc tgtacgggac ggccgttttc
     1381 ctccacggcg ccctgcgccc tgtaaccgct gcccgagacg ttcagtgtgc gcccgggcac
     1441 ccagatgcgc tgtaccgtca ttttgttctg cgtgatggtg ccggttttgt ctgtgcagat
     1501 aacgcctgcg cagcccagtg tttccacagc gtgcagcttg cggatgacgg cgccgcgccg
     1561 caggatgcgg ttgacgctga gcgccagcgt gatggttacg atggcgggca ggccctcggg
     1621 gatggcggct acggcgaggg agatgcctgt gagaagcatc tccagaaaat cgttgccctg
     1681 caaaaagccc aggaagccga cggccagaca gatggtgacg caggccacgg ccacgaaccg
     1741 tcccagctgt ttgagcttga gctgcagggg ggtgggctcc tcgccggcgg tttccatcat
     1801 cccggcgatc tttcccatct cggtctccat gccggtggcc gtcaccacgc cttcgccccg
     1861 gcccgtcatc acgatgcagc ccatgtacag tctgtctccg ggctgctttg cggcgggaag
     1921 gctctcgccc gtgagcatgg cctcgttgca cgatacctgc acaccggttt gcagcgtgca
     1981 gtccgccgct accttatcgc cggcctcgaa cagcagcacg tcgccgggca ccacggtacg
     2041 cgcgccgacc tccagttccc tgccgctgcg gcgcacgcgc gccgtgggcg ccgaaagctc
     2101 tttcagtgct tccagcgttt tttccgtgcg gtattcctgc agaaagccca tggtggcgtt
     2161 catcagcacg atgattataa tggtaatggc ttccgtgccc tcgcccatca gcacagagat
     2221 gagcgtggcc gccgccagga tcaggatcat caggtccttg aactgcgaga agaacatggc
     2281 ggccacgctg gctccctttt tcccgcgcag gcggttttcg cccactttcg caaggcgggc
     2341 ttttgcctcg gcttcggaca agcccgcaga tgtgttttgc agcatgcgtt tctccccttt
     2401 tttgatttgg gcaaagaggc ccatgattgc taaaacttat gaaaaaatgc gccgcgctat
     2461 gccggacgaa aatttttctg aaaatttctc ccgattttgc agacaaatcg gccgctgtct
     2521 cgtattatag gcatggaatg aaaaaaacac ggaatttacc gggcttcggc cctttaaagg
     2581 agatgaaccc caaatggtga ctttgattct gacacggatc gtttttgtgc tgagcatccc
     2641 gctttgcctg tacgccgttt atttcggtgt ggtcgcactg ttcggcctgc gcaaaaaact
     2701 gcctgcggcg ccccaggcga agcctgaaaa acgctttgca cttgtggtgg cggcacgcaa
     2761 cgaggcggcg gtgatcggcc atctggtgga cagcctgtat gggcaggatt atccccgcga
     2821 gctgtatgag gtgatcgtcg cgcccaacaa ctgcaccgac gacaccgagg cagtggctgc
     2881 cgggcgcggc gcgcgcattt tccgtccgga gggcgtcatc cgcagcaagg gcgaggtgct
     2941 cacccaggtg gtggataaaa tcgtgctggc ggaggagttc gatgccatgt gcgtttttga
     3001 tgcggacaat ctggtggacg gccagttcct ggcgcgtatg aacgatgcgc tggtgagcgg
     3061 cgcacaggtg gcgcagggtt tccgcgacag caagaatccc gaacagagcg cgatctccgg
     3121 ctgttattct atctgctact ggatgctgaa ccgtttctac aatggtgcgc gcagcgcgct
     3181 gcacctctcc gcgctggtga acggaagcgg ctttgccgtt accactgcgc tgctgcgccg
     3241 tttgggcggc tggcacacgg ttaccatgac agaggattat gaattcagcg cccagtgcgc
     3301 catgctgggc gaacgtgtgc actttgtgcc cgaggccctc atctacgacg agcagcccct
     3361 tacattccgc cagagctgga agcagcgccg ccgctggacg accggcagcc tgcaggggct
     3421 gcagaactac ggccacggcc tgttcagcag ctttgtactg cacggcagct tgatctgcct
     3481 ggacatgtac ctcacgtttc tcgtgccgat ggtgcagctt gccagcgtag tgctggggat
     3541 cgcggggctg gtgctcacgc tgttgggcag cggcatcacc ttcggcggca ttatgctgca
     3601 cggcatgatg ctggcggcgc tgatgacggc cggaggcttt gtggcaagcg tggtgggctc
     3661 ggcggtattc gccgcgttca ccgtggcgct gaagaatgaa tcgctttccg gcatggccaa
     3721 gggcattctg gcctattggg tgtttctgat gagccagatg gtgctcacgc tgctgagctt
     3781 tgtgaaaaag caggaaaaat gggaacccat cgcgcatacc agcgcaaagc ggctggagga
     3841 catgcgcacc gcctgagacg ggcggcggcc gtctcaggcc atcgggatgg act
//
LOCUS       DAWBSB010000210         3892 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_3946_length_3892_cov_3.038311, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000210 DAWBSB010000000
VERSION     DAWBSB010000210.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3892)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3892
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3946_length_3892_cov_3.038311"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          795 a          978 c         1401 g          718 t
ORIGIN      
        1 ggtctgctgg ctctgccgtt cgggttggct ctgatggctg gtttcctgct ctggaagaaa
       61 aaaagcggca cacgctgaca catattggaa cgaaaggccg tccgcggcgc ataccgcgga
      121 cggcttttta attgcagagc ggtgaaaaga ctgcccaaac ggctcgccgg atggcatgaa
      181 aaacggcggg gcttttgatg tgcaagaaat acaatgtact tgatgcgcgt aagagggtat
      241 tctttattgt aatggaattg ctggaaaagg ccgggcggaa agcgccctgc cggagccgca
      301 ccgtcaggaa agaaagcagg gaggaaagcc ggtatgaaaa accattttgt acatacgccg
      361 tgcgggcccg tacagggcgc ggcgggcgga gcggacggcg tgtccgtatt ccgcggcata
      421 cggtacgcca cggcagggcg ctgggaatat ccccggcagg tgacgcattg ggacggcgtg
      481 tatgacgccg ccaatttcgg tgcgtgttcg tttcagccgc gcgcgttcta caacgaagaa
      541 gacgtgccgg aaaaggcgtt ttattacaac gaattccgtc ggggcgaaca ctacgattac
      601 agtgaagact gcctgttttt aaacatttgg gctcccgacg acgcaaaaaa cgcgcccgtg
      661 ctgttttata ttcacggagg cggatttttg ggtgggtgcg gccatgaaaa gcattttgac
      721 ggcacggcct ggtgcaaaaa gggcgttgtt gccgtcacca tcaattaccg tctgggccct
      781 atgggctttt tgtgcctgcc ggagcttgcc gcccgggacg ggcatacggg caactacgca
      841 atgtttgacc agatggccgc gctgtgctgg gtgcgggaca atatcgccgc ttttggcggc
      901 gacccgggcc aagttactct tatgggccag tccgcgggcg cgatgagcgt acagcagctt
      961 tgtgtttcgc ccatgacgaa cgggctgttc cgcggcgcgg tgatgatgag cggaggcggc
     1021 gtttccagac tgctgggcgc ggcgcagaaa gcggaagaag cgtatccgtt ctggcaggcg
     1081 gtgcggcaga agctgggcgc ggcagatttt gcggcgctgt gcggcgcaga accgcaggcg
     1141 ctgttcgcgg cgttcgccga ggcaaggaaa gaacagaaga accccatggc cgggtgcggc
     1201 ccggtggtgg acggcgtatt ccttacggaa agcgcggcgg aagctgcaaa agcagggcgg
     1261 caaaagaaca tcccgtacct gatgggctcc accagcgagg acgttgtgcc gcccatcgtg
     1321 cataaaatgg caaagggctg ggcgcggttg caggcggacc agggcaaaca gcccagctat
     1381 gcctatttct tcagccgtca gctgcccgga gatgaaaaag gtgcgtggca ttcggcagac
     1441 ctgtggtatg cgttcggcac gctggacagc tgttggcggc ctttcacgga atgggaccgg
     1501 gctttgtccg aagcgatggt gtcgtacctg acgaattttg caaaaacggg agaccccaac
     1561 gggccgggat tggcggaatg gctgcctgtg aaaaagggcc agacgcaggt gctgcggttt
     1621 ggagacaggg atatcaccat gggcggcgtc tctgttgcca ggctgacgca caccatgctc
     1681 accaagccaa acgtgggcga atgacgggag agcgaggtgc acggtatgta tgacgccgtc
     1741 gtcgcaggag cgggccccgc gggagcggca gcggccaagg cgctctgtga agcgggccgc
     1801 cgtgtgctgc tggtggagcg gtttcggctg ccgcgataca aatcctgctc tggcatattg
     1861 atcaaaaagt cgttggggct ggtgaagcag tattttggcg aggaagtacc gtcggctgtc
     1921 tgctgtgctc cggtggaaaa ccgggggatg atctttaccg atgacaaggg cggggaatat
     1981 gctttcaggc aggaagggcg caatgtgtgg cgcggcgctt ttgacggctg gctcgtggcg
     2041 aaggcggcgg aaagcggagc ggagctgctg gacggtaccg ccgcgattgc ctgtgaagaa
     2101 gagaacggcg ctgtttgggt cacgctgcag aacagggaac gctgtaaagt gcgggcaagg
     2161 tatctgctgg actgcgaggg cgtgacgggc gcgctgaaac ggaagctggc gccgggcccg
     2221 gcgcagtata tcacaacgtt tcaaaccttt aacgagggcc gcatcgcgct ggacccgcat
     2281 tatttttacg cctatcttca gccggagctt tcggagtacg acgcatggtt caatgtgaag
     2341 gacggctttc tggtgctggg cgtgtcggtg cgggatgcga agcgggcgga atatttttat
     2401 cggcgtttcc tttcctacat ggaggagcgg cacggcctgc gcaccggccg ggaggaaaga
     2461 cgcgagaaat ggctgatgcc ccgtattccg cccggctgtc ccatctatta tggacaggga
     2521 cgcgttttgt gcgcgggcga agcggcgggg tttctgaacc cgatgggcga gggtgtctcg
     2581 gctgcgctgg agagcggaca tcatgcagcc ggcgcgctta tggagcattg gaatgagccg
     2641 gagcgcgccc tggcgcgcta ccgggaacgg acgaacgccc tgcgggctta catgcggcgt
     2701 caatggagtt ttaccgcagg catggcggag acgttccggg agatgaggac gtaaggagac
     2761 cgcctgaacg ccgaatgacg ggaaaaacgg caatgaacgc ctcaaaaaca gacggccgct
     2821 cttgacggcc tgcctgccgc cgcgttatgc tgaatacagc aaagcggtgc gtccgggctg
     2881 cggccgcacc gaaaaatgag aggatgtgtt tttttggaac gcaatataaa cgagcttgtg
     2941 gcgcagatga cgctggagga aaaggccggg ctgtgttcgg gcgcggattt ctggcatttg
     3001 aagggcgtgg aacggctggg catcccgggc gtgatggtgt cggacggccc gcacgggctg
     3061 cgcaagcagg accaggaggc ggaccatctg ggcatcaacg aaagcatcaa ggcggtgtgc
     3121 ttcccggcgg catgcgcgac ggcggccagc ttcgatgagg cgctgctgga acggatgggg
     3181 cgggcgctgg gcgaggagtg ccgcgcggag gatgtgtctg tgcttttggg gccggcggtg
     3241 aacatcaagc gcagcccgct gtgcgggcgg aactttgagt atttcagcga ggacccgtat
     3301 ctggcgggaa agctggcggc ggcgcaggtg cgcggtgtgc agagctggga tgtgggcacg
     3361 agcgtgaagc attatgctgc caacaaccag gaataccggc gcatgacgtg ctcgtccgac
     3421 atgagcgagc ggacgctgcg ggaaatttac cttgcgccgt ttgagacgat cgtgaaagag
     3481 gcgcggccct ggacgctgat gtgcagctac aacaagatca acggggtgtt tgccagtgaa
     3541 aatccgcaca cgctcacaga gatcctgcgg gatgagtggg ggtttgacgg atatgtgatg
     3601 agcgactggg gtgcggtgaa cgaccgcgta aagggcctgg ccgcagggct ggagctggaa
     3661 atgccatcgt ccaacggcgt gcgggacgcg cagatcgttg cggcagtgcg cggcggtacg
     3721 ctggaggaag cggtgctgga caaggcggtg gcacgcattc tgaacatcgt gttccgctat
     3781 gcggatgtac agcatccgga ggcgaagttt gaccgcgcgg cacaccatgc gctggcggca
     3841 gagctggaaa aagaatgcgc ggtgctgctg cagaacaagg gcgcgctgcc gc
//
LOCUS       DAWBSB010000211         3876 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_3958_length_3876_cov_2.037686, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000211 DAWBSB010000000
VERSION     DAWBSB010000211.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3876)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3958_length_3876_cov_2.037686"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2476..2485
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2822..2831
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3086..3095
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          886 a          998 c         1191 g          771 t
ORIGIN      
        1 caggtggtgc agaactttgt ggaatacgtt gcctgcggcc tgacgaacct gatcaatatc
       61 tttcagccgg agatcatctg catcggcggc ggcgtatcca aggaaggcga tacgcttctt
      121 gcgcccgtgc aggcggtagt ggaccaggaa gattacgccc gagagggcat gcggcgtacg
      181 aaaatcgtca ctgcaaagct gcgcaatgac gcgggcatta tcggtgcggc cgggcttgcc
      241 acgcagcatc aatgattttg gagggattcc ccatggagca cttgaacgag atcgctgcgg
      301 ctttccgcca ggcatccatc ccatgcgctg cccgcgaacc gctttcgcgc cataccagct
      361 tccagatcgg aggcccggcg gctttgttct gtgagccgca gaataagcgg cagctcgcgc
      421 gggccattgc cgtctgccgc caggcgggcg tgcggtatta tttgttgggc aagggcacga
      481 acatcctgtt cgcggacgag gggttcgacg gcgtggtcat ccacatcggc gaggtgctgg
      541 ggaacatcga atgcaacggg ctctctgtga cggcgcaggc gggggccgct ctctcaaagg
      601 tgtgcatcgc ggccgcaaac gaagggttgt ccggcctgga attcgcctac ggcatcccag
      661 ggtgtgtggg cggcgccgta tatatgaacg cgggcgctta cggcggcgag ataaaggatg
      721 tgcttgcctg cgcaacgttt ctggatgaaa ccggcgcgga gcggacactt caggccggcg
      781 agctgcagct tggataccgc acatctgttt ttgagcgcga gccgtggtgc atcctgtccg
      841 cgacgtttac gctgcgggaa gacgacacag gccccatccg ggagcggatg gcagattacg
      901 cccgccgccg catggagaaa cagccgctgg atatgcccag tgcgggcagc acgttcaagc
      961 gcccggaggg cgcatatgcc ggagcgctca ttgaccagtg cgggctgcgg ggatacgcca
     1021 tcggcggcgc gcaggtgagc caaaagcact gcggttttat tgtgaacacg ggcaacgcca
     1081 cctgcgcgga tgtactgtgc cttgtggata cagtgtgcaa gatcgtgaca gacgaaacgg
     1141 gcttccggct ggaaaaggaa gtcagagtcg taaaataaaa atggggtaaa gggacgtatg
     1201 aacctattga tcgtgaccgg gctttcgggc gcgggcaaat cgctggcaat gcacgcgctg
     1261 gaggacatcg gctttttctg catcgataac attccggcga atcttctggc gaagctgatg
     1321 gaatttgccc agcagagtga aaatacgctg gagcgcgtgg ctgttgtgtt ggacatccgg
     1381 ggcggaaaat cctccgagga tattctaaat gcgcttggcc agctgaaagc cgggcgggtg
     1441 aactataaaa tattgttttt ggacgcgcgc aacgatgtgc tggagcggcg gtacaaggaa
     1501 acgcgccgcc gccatcccat cagcatcgcg tctcaggtat ccacggatga agccattctg
     1561 caggaacggg agattctggc gccgttgtat gaaatggcgg attataaaat cgacacctcg
     1621 ctgttttcca cggctcagct caaggaccgc gtggtctctc tgtttgtgaa ccggtcctcg
     1681 gacgcgatgg cgctcacggt gacgtccttc ggcttcaaat acggccagcc caaagaggcg
     1741 gatatcgtat ttgacgtccg ctgcctgccc aacccgtttt atatccctga gctgaaaaac
     1801 aaaaccggcc tggacaaaga ggttgtggaa tatatcatgc agtttgagga ggcgcacggc
     1861 ctgctgcagc gcatggagga ccttgtagga tacgcactgc cgctctatgt gaaagagggc
     1921 aaaagccagc ttactatcgc tgtggggtgc acgggcggca agcaccgttc catcgccttt
     1981 gcggagatgc tggccgcgtt tgtcaagaag ttggggtacc atccggtcgt ggaacaccgg
     2041 gatgtccagc gcacccatac ataacccggc gcagcccggg caaggaggac gcgtcgtgag
     2101 cttttcccag gacgtaaaaa aagagatcat tgcgcgcgaa gcgggcggca gctgctgcgc
     2161 cgttgcagct gcctatggct ttgcgtgctt cggcaaatat tttgacaccc gtggcgttgt
     2221 tctgcacacg gaacagatcg ccgtagcgca gtacgcgaaa aaaatgtttg cccgcgcggg
     2281 cgtggcggga aagatttatg ttaaggggag cgaggaaagc cgcgtttatg aattcgcggt
     2341 gaaagagccg ggcgaggtgc ggcggatgct ggccctgttc ggacatacag gcgaagagcc
     2401 gagcctgcgc atcaacagca gaaactttac ctgtgaacac tgcgtgagtg cgtttgtttc
     2461 cgcggccttt ttgtgnnnnn nnnnncctaa gcacacccgg cgcaaaggct ccaatgtgct
     2521 ttatctgaaa gcaagcgagc agatcgagga tctgctcaca ttcatgggag cttccggcgc
     2581 ggctctggag atcatgaacc tgaaggtgta caaggatttt cgaaacaaag ccaatcgtat
     2641 taccaattgt gaaacggcaa atatcgataa aaccgtgtct gccagcgggc agacgctgga
     2701 ggccatcgct tatctgaaaa agcgcggcgc gttcgaaacg ctgcccgcgc cgcttcggga
     2761 agccgccgcc ctgcgggagc aaaacccgga cctttccctc aaggagctgg ccgggctggt
     2821 cnnnnnnnnn ncaaatcggg cctttcgcac cgaatgaaaa aactggagca aacggcggaa
     2881 agcctgaaag aaaggacaag gaatggctga aaaggcaaga aactttacac atctgcatct
     2941 gcataccgaa tacagccttt tggacggtgc atgccgcatt gaggggctga tgcagcgcgt
     3001 gaaagcgctg ggccagaccg ctgttgccat caccgaccac ggcgtcatgt acggctgcgg
     3061 ggatttttac aaagccgcca aaaagnnnnn nnnnnaaccg catcgacggc tccaaccatc
     3121 tggtgctgct gtgcaaaaac gagacgggct ataaaaatct tatcaagatg gtgtcggcgg
     3181 gctttacgga aggcttttac aacaagccgc gtgtggacca cgagctgctg gaagagtacc
     3241 atgaggggct gatatgtctc tccgcctgcc ttgcgggcga aatccctcag gctttattgg
     3301 caggggatta tgaaaaagcc aagaatcttg cgcggtacta tgaagatctg ttcggcaaag
     3361 gaaactatta tattgagata caggaccatg ggctggacga gcagcgcacg gtgctgcccc
     3421 tgcttgtgcg gctttcccgg gaaacaggca ttcctctggt cgccaccaac gacgcccact
     3481 atcttgaaaa agaagattcc aggatgcagc acattctcat ttgcatccag acaaacaaga
     3541 cggtgaataa cgatgacgta ctggaattcg gcacggatga gttctacgtc aaaagcaccg
     3601 acgagatgta cgagctgttc agcgcatggc ccgaagcctg tgaaaacacg aaccgaatcg
     3661 cagagatgtg cagctttgat tttgaattcg gcgtgacaaa gctgccgtat tttgtggcgc
     3721 cggacggcat ggacaacaaa gagtattttg taaagctgtg ccgggacggt ctgctgcggc
     3781 ggtacggtgc ggacgtgccg gaggatatac gcgcccggct ggactacgag atatccatta
     3841 ttgaccgcat ggggtatatc aactattatc tgattg
//
LOCUS       DAWBSB010000212         3871 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_3964_length_3871_cov_3.947851, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000212 DAWBSB010000000
VERSION     DAWBSB010000212.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3871)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3871
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3964_length_3871_cov_3.947851"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          602 a         1224 c         1287 g          758 t
ORIGIN      
        1 gaagcgaccg ccgaaggccg ccgtgcgcgg cgaacggggc cgcaggccct gttcgcaggc
       61 agcttggcgc aaaagcgcag ctttttcgac aagctgcgaa atacactcag atcataaaag
      121 gagttcgtct tatggcccga tatgaatttt tccttgcgtc ttctctggag aaggtattcc
      181 cggcacagcg ccccgcggcg ctttcggacg gcgcgcgcct ttccgtctgg cggggcgccc
      241 gcgccgcggt gcagctcgta taccgtgcgg acgacgccgc gccgggcatg cccgtgcaga
      301 gctttaccat cgaggcggac ggcgcgcctg cgcccgccgt tctgcgcgcc gtggagctga
      361 tgccctccga ttatccctgc tacgaaagcg cggacgagca ctacatcacg aaggagccgg
      421 gcctgttccc ggacctgctg gccccgctgg aggcgcccgc ggttacgccg ctgccgcgcc
      481 agtaccgcag cgtgtggctg agctgggata tccccgcgga cgcggccccc ggcgcgtatg
      541 aggtggccgt gacggcgcgg gccgtggagg agcagaggat gcccaacggc gtgctctacc
      601 gggacgcaga cgcggccggg cttgcgttca cctgccgctt tacgctgtgc gtgggccggg
      661 cgcggctgcc cgcccagacg ctgctgcaca ccgagtggtt ccatgcggac tgcctggcct
      721 cttattacgg cgttgcgccg ctgagcgaag agcactggcg catcctggag aatttcatcc
      781 gcgcggcggg ggaggagcac ggcatcaata tgctgctcac gcccgtgttc acgccgccgc
      841 tggacaccgc cgtaaacggc gaacggctga ccgtgcagct tgtggacgtg cgccgcgacg
      901 ccggggtgta ttccttcggc tttgaaaagc tcgggcgctg ggccgggctg tgcaggcgcc
      961 acggcgtgga atatctggag atcgcccatc tgttcaccca atggggcgcg cacgccacgc
     1021 ccaagatcat ggccgtggtg gatgggcagg agcggcgcat cttcggctgg gacgtgccgg
     1081 cggcaagcgc ggaataccgc gcgtttctgg aggcgttcct gccggcgctg cgcaccgcgc
     1141 tggagggcat gggctacgac aaagagcatg tgtactacca catctccgac gagccgtcgc
     1201 ccgagcatct ggattcctac cgcgcggcca aagcccaggc ggaggatttg ctggagggct
     1261 gccaggtcat tgacgcgctc agcagccttg aattttacag gcagggcctt gtgcgccagc
     1321 ccgtgcccgc caacgaccac atccagccgt tcatcgacgc gcaggtgccc gggctgtggg
     1381 tgtactattg ctgtgcccag agcagccttg tgcccaaccg ctttttcgcc atggagagcg
     1441 cgcgcaaccg catcatgggc gtgctgatgt acctgtacgg catcaagggc ttcctgcact
     1501 gggggtacaa tttttacaat tccaagtttt cactgcatcc tgtggacccg taccgcgtga
     1561 cgcacgccga ctatgcattc ccctcgggag acccgttcct tgtatatccc ggcccggacg
     1621 gcgcgccgct ttcgtcggtg cgcgcggagg tgcaggacga cgcgctgctg gacctgcggg
     1681 cgctgcagct gctggagcag cttgccgggc gcgcgttcgt ggaggagctg atctatgcgg
     1741 acgcggagat gcggcccatg acattccggg attacccgcg cggcgccggt tacctgctgg
     1801 ccctgcgcga gcgggtggcc gctgagatcg agagccggct ggcttaaggc aacaccgcac
     1861 aattcacggc tgaagcctta agcgtcgcct cgcttgcaga ttttccggca actgtcacaa
     1921 aaatgctcgc gaatacacaa agtattcgct gcgctttttg tttaacctgc caaaaaatct
     1981 gccggcgcga ttcgactctt agaattgtgc ggtgttgcct taaagaaata ccgcgccggg
     2041 aacggcgcgc cgcccgtagg cttgagcgcg ccgccttccg gacagcctga aaagcgcggc
     2101 tgtaaggcca accccggggc cgtcctgcca aatgggcgcg ggcgcagcgg cccgctccgc
     2161 aggacggccc ctgtcctttt cccgcggcgc gggaacaaac aaaacaccat ccaacaacag
     2221 aaacggagcg cgtttgttat ggaagcacgg taccgttcca cgctgtgggc ctgctgcttt
     2281 gcgaattttt ttcaggctgt gggcagctgc tttgccattc tgtacgtgcc gctgcgcggc
     2341 ctgtacggcc tcagctttac cgatttcggc atccttgtct cgctgaattt catcacgcag
     2401 gtcgcgtccg acgtcctgtt ctcaaagccg gtggaaaaat acggcttccg cccgtttgcc
     2461 gtggccgctc ccctcgtcag cgcggccggg ctgggcctgt ttgccgccgc ccccctactg
     2521 ttcccccgga ggccctttgc gggcttttgc gcgggtatgt tcctgttcgc cgcggcgggc
     2581 ggcctgcagg agctgctgct cagccccatt ttggacgcgc tgcccatccc ggaggagcgc
     2641 aaggcccgca gcatgagcat gctgcactcg tttttcgcct gggggcagat cttcgtggtg
     2701 ctggccacca cgggcctgct ggcggtgtgg ggcgccgcgc gctggcaatg gatcgtgctt
     2761 ttgtgggcgg cgccgcccgt ggccggcgcc gtgctgttct gccgcgtgcc gctggcccgc
     2821 aaaaacggcg gcgccccgcc cctgccgctg cgggcgctgg cccgcaaagg cgtgttctac
     2881 gcgctgctgg cggccatcgt gctgggcggc gcggccgagg tgaccatggc ccagtgggcg
     2941 tcggcctttt tggaaaaggg cctgcggctg ccaaagctgg cgggcgacgt gtggggcgtg
     3001 tgctttttct cgctgatgct tgcgctgggg cgcagcggct atgcggcgct gggcgcgcgc
     3061 gtgccgctgc accggctgct catcggcggc agcggcgcgg ccgccgtgct gtacgtggtc
     3121 tgcgcgcttg cacaaagccc tctgctgggg gtgctggcct gcgggcttac gggcctttgc
     3181 gtcagcctgc tgtggccggg cgcgatcatt cttgcgtccc ggcgcatccc ctccgcgggg
     3241 gcggccatgt tcgcgctgct ttccgcgggc ggcgatgtgg gcgcgtccct gggggcgttc
     3301 tgcgtgggcc gcgcggcgga ctgggcgcag gcgtccggca cggtgtttgc gggggccgcg
     3361 ggggaatctg gggcgctgcg cgcgggcctg ctgctggcgg cggtgttccc gctgctgtgc
     3421 tgcggcgtgc acacggcgct gcactgcagc gaagcgcggg gcggcttacc cgcagggagc
     3481 gaacggcggc acaggcaata gaagaaagct gtttttcaaa ccggaaccgc cgtttgcaga
     3541 cggggcgaaa gaccgcaaaa tgaacaaacg gcgcggcctt catggccgcg ccgtttgtgt
     3601 gccgggagga cagccgcaaa gcggcgaaag ggatgcggtt tccggtctga caaacgggaa
     3661 tttatgaatc aagtttatat atcatgtctc ctatattcca aatattcatt attccactta
     3721 caggttcttt ttctggacgc caaatccacc ctgaatatat tgtacagtac ccattatatc
     3781 cttctgtcaa aatttcaaag taagcatctg aatactgatc tatataatcc tttaaagtga
     3841 ttgctttccc ggaaaagttc ccttcattaa c
//
LOCUS       DAWBSB010000213         3853 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_4001_length_3853_cov_3.127962, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000213 DAWBSB010000000
VERSION     DAWBSB010000213.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3853)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4001_length_3853_cov_3.127962"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    329..338
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          707 a         1203 c         1097 g          836 t
ORIGIN      
        1 ggctgcacca acccgcccaa gaccgagacg gagaaccata tcaaaggcaa ctggatggca
       61 gactccaacg acgccaccaa aatgcacatt tactgtacac agcccacatg tcacgccatt
      121 cttgacactc atacccacag ttggagcggg tggacggacg ccggcgatgg cgtaaaccac
      181 aaacgcacct gctccgttgc cggatgtccc gcccctacgg aaacacaggc ccactcctgg
      241 ggcgcaacgg accaggctac tggtatgcgc acctgcactg tctgcggcgc ggccggaaag
      301 gaccccaact ggaccgcgcc gcccgccgnn nnnnnnnnca tgtccatgcc tggagcggct
      361 ggacggatgc gggtgacggc gcgaatcaca agcgcacctg ctccgccgcc gggtgccctg
      421 cacccacgga actgcagccc catacttggg ggacgacgga tccaagcacg ggcgcccgca
      481 gctgtacagt ctgcggtgcc ggtggacagg atccaaactg ggctcctccc gcaactacct
      541 ctatccccgc ccctgccgca tagcttttcg ccgcctgccc tttttgtggc gggcggcttt
      601 cattttttct atcaggcaga aagaaggttt tttatgctca acagcaaggg tttcgacgaa
      661 tgggccgccg gttatgacgc cagcgtccgc cattcccacg atgcggggga atatcccttc
      721 gcgggctacg atgcggtgct gtctcttatc gaggcccgtg tgaacagccg tcccggcgcc
      781 gatgtgttgg acttgggctt cgggacaggc atgctgacag cacgcctgta cgcggccggc
      841 catccggtag ccggagtgga tttttcccag aatatgctgg acgccgcccg cgccaagatg
      901 ccctgcgcgg aattgtatct gtatgatttc acacggggcc tgccccccgc gctggagggc
      961 aggatgttcg attttatcat cagcacctat gctttccacc atgtgccgga cgccgggaaa
     1021 cccgcgttcc tgctggaatt gtcccgccgt ctcagccccg gcggcgaatg tctgatagga
     1081 gacgtggctt ttgaaacgca ggccgagctg gatgcctgcc atgccgcggc cgggacggcc
     1141 tgggactgcg gcgaggatta tgccgtggcg cagctgcttg ccggtgctct gcccagcctg
     1201 cgcctttcct tcgcgcggct ttcccactgc gcgggggtgc tttccatcgg gcggcacgac
     1261 tctccgcagc ctgtttgacg caaaaaacag ccaagcatcg aatgagcttc attgcaaacc
     1321 gataaaaaga ggccggagcc cggcgggaaa atcccgccgg gctccggctt ttttatctca
     1381 gctccaagtg agcagcgcgc gccgcgccta ctttctaccg ccgtaaaagc ggcggtacgg
     1441 gttctgcgtg cgctgggtgg gcgtaagctg ctccgggcgc ggcctggaac ggctggccgc
     1501 cactgccgcc agcaccagca cgcccagaat caccagcgcc gcggccaccg cagccatcag
     1561 gatccactgc cagggaagca tacgccttcc tccttttccg ttcgttcact gccgcgtcag
     1621 agtatggaac accgacatcg ccttttcgta atacgcggcg gcgtcctcat ccttttccag
     1681 cttggtgccc gtataaacgc ggggcaccag cttgtagtcc tgcaaaaatg tctggtaatc
     1741 atacccctgc gaaccgtcga agttctccga cgcgcccagc tccagcgacg tgagcgccgg
     1801 acagtctccc cagcgcacgc ccatgcgcgc atagtccggc ttttcgccct ctgcgggaga
     1861 tgtgccgtcg ttgtaggcga acagcatata ctgctcctgg aaatactcca tatcgtccac
     1921 aaggaacagc atgctctcgc cgccgtcgat gtcgccggac aacttcgtga tgttcgccat
     1981 ctggacgttg gggtccgcga tggaatccgc gtctttcccg atggtgtatt ccatcacgct
     2041 cacaacgacc tctccgtcgc cgttgcggtc gtcaaaatac ggcttgagct gctcggccag
     2101 tgcctcgccg gtgcccatgg gcacacctgt cgtggtgagg atgccgatgg tataatccgg
     2161 atgcacctgg gtggcgatct cccaaacgaa cgaaaagacc atcagcgcgg ccacgatgcc
     2221 gacgatcaca tgccatttgt gatagaacca gaaattttcc cgtttttcct tgccggtcat
     2281 ctcatgcttc ggctgttcgg gctgcaggtc ctcgggcttg atgttcagtg tgtagcgcgg
     2341 gcttgccata cacgtgtttc cctctttctg ttatatgtgt cgcggcgggc ctgcccgcac
     2401 gcttttatgc atacgccgct gcgcggcgtc agtgcgctgt ggttttatcc ttgtgcgcgg
     2461 caatgcgccg ggccacctcg tccggcagcg ctttctgcgc cgcccggtcc agcccggcgg
     2521 tctcgatggg cggcagcacg gtcatggtca catggccggg acacatcagg ttgtggtttg
     2581 cctccatgat cttatagctc ccgtcgatga cgacgggcac cacgggcgca cccgccgcaa
     2641 aggccatttt gaacgcgccg tgcttgaact cgcccatggc gtcgcccttg ctgcgcgtgc
     2701 cttcggggaa cacgatgaca ctttcgcccc gcgccagcag cgcgcctgct tccttcatgg
     2761 ccgtgacagc ctggcgggga tttttgcggt cgatgaacac acagtcgaac agcttcatcc
     2821 aggtgcgcac cagtgggatc ttcaacgtct ccaccttcgc caccagcgcg tggggcctgt
     2881 ccagatgcac cagcgtgatg gggatatcat aatcgctctg gtggttgggc gtgaatacca
     2941 cggcccggtc cttcggaatg ttttccagcc cgttcaccgt aacggtacac ccggcaatgc
     3001 gcatcagagt ggacatccac atgggcacat atctgtccac gatggcccgg cagccgctct
     3061 catcccccgc ggccttgcgc cgctttgcct cgttcatttt cggtgtaagc acgatgagcg
     3121 cgccgaagaa atagaaaaac cacacgatcg tccgcaatat tgccataccg gtatcattcc
     3181 ttttccgcgc cgggctttcc gcgtccggcg gcctgcatac tttttctatg ataccgcaaa
     3241 ccgggcgaat acgcaaacag ttttgccgcg cagcatggcc tcggtgctgc tctgttgtta
     3301 gtatagcccg attttacaca aaaaaagtgg cagagttgtg aacaagcggg aaaaaaatcg
     3361 tattttttcc ttttttgtta tacggcccgc caatatttgt atttttatcc gcgctttttg
     3421 tctgtttggt cttgtgcata tattctaaac tttacggtct gattttggat gcgccttgta
     3481 caaaggcgca attttgccgc cgcgggcttg acatgccacg tcccggcgcg taaactgaat
     3541 gcaacaacag cgaaggcgct gccggacatc cggcggcgcc ttttttgttg tatttccgcg
     3601 gttgaaaagc gtaaaaaatt gaggaggggt cattatgtat tcatccgtag acaccatttg
     3661 ggtgctgctg ggcgcggcgc tggtattctt catgcaggcg ggctttgcga tggtggagac
     3721 cggcctgaca cgcgccaaaa atgcgggcaa catcatcatg aaaaacctga tggattttgc
     3781 cctcggcacc gttgcctttt ggctggcagg cttcggcatc atgttcgcgg gggacggcgc
     3841 gttgatcggc ggg
//
LOCUS       DAWBSB010000214         3816 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_4033_length_3816_cov_1.505717, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000214 DAWBSB010000000
VERSION     DAWBSB010000214.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3816)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3816
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4033_length_3816_cov_1.505717"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3553..3562
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          969 a          947 c         1129 g          761 t
ORIGIN      
        1 gaatctttat gacatcctgc gtcacgggct cggcatggag cacagcgaca tggaggccct
       61 gggcttccag ctccgcgact actacgagga ggttgagtaa tatggcatac caaaaagtat
      121 ccagaccgtc cacggtctat catttgacgc aaaaaggcaa tctcgacagc attctggatg
      181 acggcgtgat ccgccgcttt ggtgatacgg aatgttggtt ctgcgagagt ctcgacaaaa
      241 tgaaagcgta catggagcag accgtgatgt gtaagggcaa accgtatcgc accgcagatg
      301 gacagctctg ccattactcc aaattcaacc cggaagcata cattcttctc aagcttgctc
      361 ccatccggca ggaaaatgcc tggtatcgcc ggactcagga acagtcttca agcagttctg
      421 atgaaatgat gcaggcagaa agagaattct tcccactgag tattggatat cgcggtgata
      481 tggcatttca aaatgcggag gtcatcgatg tgccaaaatt ccttgccggc gaaatcgtca
      541 gccacaagga actgacctcc agtgaactgt ggggcctgtt atttgagcga accgaggtgg
      601 aaatggctgc ctatatgcgt gggctggatc atctggaacg tgacgaactg atccagtccg
      661 cggcggagat ctccgccatg caggtgtgcc gcagaggact gatggcggag aggaaaaaac
      721 tgacaaggag aaacgttcta tttttgctac aggtggaaaa gccgctggga atgatttctg
      781 aagtgtggat ggagcaacag aatgccggag aaggtaatgc tttttcccga ttgcttgcgg
      841 aactgtacaa acagaaagga aatgggtatt tgaacaatga tgaacagcca gaaaccgtga
      901 agcggatgct ggaatcctgt ccaaacaatt gcttcatgct tctgatgccg caaggtattt
      961 taaagcttgc ctccgatgaa gctgaaaagg ctctgcgcgg tgaagaatac actgtacttc
     1021 tggactattc cggagattcg gaaccgatta agacagcagg cttgcttgaa atggtagtca
     1081 ttgatatgag gaaggatgag cagggctgct ggtatgtcct ggttgtccat ccaaagctgt
     1141 tggaggcgct tagacgggaa aagaaaatga cctgatatgc tcattactga cgaacacatc
     1201 gaaacctatg cgatgatcga ccacggcaac ggactcacca cattgtacac agattctctc
     1261 taccgcatga cgcagggaca gcttattgct aacgccggag agatcaaggc gaaccgtttt
     1321 tgttataacg ccttgctgac catgtgttta gaccgcgaac agttgaaagt gctggcggct
     1381 atggataacc cgctggaggc ggtgtgcggc gcatgggcct cggcgcagga ggtggaacag
     1441 gaggaagaat tctctcatac gctgtttgaa atctgtgggc agacggcgca agaacagacg
     1501 atgcggatga aataggagga acataccaat catgaaaaaa gaaaatgtca cgatctcatt
     1561 cgagagtgag cagcttggat gagctgctca aatgccttta tgaatccgag gtgccggagc
     1621 cggtgcggga gtatctggac agccgcgcgg cgcccgctgc ccgtccccgc cgtccggcaa
     1681 gaccggcgaa gccgcagccg cgggaggaga ttccctccgc gttgacgcag cccactggcg
     1741 aaaggaatgg tgagtgagat ggcagatcat gtagacatca cactgtggat cgaccggcga
     1801 tggaaggatg ccattgaaaa gcatctcaag gatgaaacct tgcaggagca tctggagaat
     1861 atgctggatg agatgtgcaa ccaactcccg gagcgtgagt atgagcgcat cagtgccgag
     1921 atctatgcgg aggacatcgc ggagcgtgaa gcgagggagg ctgcccgtac ctatgccgct
     1981 taccatgtgg tagagagcgg acaggagtgg tacttcaaga cctcccccgg tgaggaactg
     2041 ctggatgccg cagaaaagct gcgcagctat gtcactgcgg agaaaggctc cgcgccggat
     2101 ctgttcatca agctgttcgc ggacgggcat cccatcatag cggaagagta caacgcgctc
     2161 accgcccttc gcatggaaaa caccggcaag gtgcgcggcg tgtttgatct cagcttcgac
     2221 aagcgggagt tctccgccgt acacatcatg aacggttggc agacctgggc gatgcgggat
     2281 gtatccgtgg cggcgtacca tgccacccgg tcacaattcg cgtcgagcga tgatcagtgg
     2341 cgcaagttgc tggatcatct gagcggtaag gaaatcacct cggcgggaca cctctccgcg
     2401 cggatttttt ctttcggtga tgagatcatc gagatggacg gcaagctcaa tttttatgtg
     2461 cggacggagt tcgatgtgga tgcagccttc ggcaccttcg tctgcacgga taaaaacgat
     2521 gactggctca acatctacgc caactacgac atggaggaaa accgcccctg cgacacgctg
     2581 gaactgaatc tctgcaaagc ggatggcaca gaggaaacct ggagctaccg gctcaacgcg
     2641 gcggagcggg aggtgctgct gcagaagatg gaggaatact gtcagaagca gactgacatg
     2701 agtctccgcg actacgcaca gcagttccga gaggaaccgg aacagcggca ggggccggtg
     2761 atgaaactgt aggcacaaac acagcaaggc tgaaacgctc gcggctgcgg aaacaacagc
     2821 gtccaggctg cgcagcccag cggggccgcg gcaccgccga accgcgtcaa cccgccctcg
     2881 gcgggcagcc gcagttcggc ggctgttatt ctgctccctc ggtttttctg cgatatggaa
     2941 aggcgcaaaa cctcacaaaa aattttgttg gatttcgtga atagctttcc gactgccgct
     3001 gtgccattgt gtttgcgcag cgcgctacac aaaaaataaa caggaggaat ttgaaatgaa
     3061 tacagaagcc gcaatggaac tacaaatgcg tctggcaaag gaggcgctgg caatgctggt
     3121 gattcatcca acgtttgacg tgcagttgta ccgtgagagc atcatggaga tcggtgaggc
     3181 gtgggaactc ccagcagacg caacgctgga ggcgctcgca ctcatcgaac atgaacgatt
     3241 ggcgattcaa aaagccggag agggcggcgt tgtgcagcac attcttccgg aagaaaaact
     3301 gcccatgcat gctaccggca cggaaacact ggataatgta tgggatctgt ttgaaacatc
     3361 cctgcgcact gagagcacga aagggcgcac ggttctctac aacatggcga ggacactgga
     3421 agaaacgcag aacctgctgg actggattga aaaaacggag gaggaaaaac aggtgtaact
     3481 gcgcctgacc agagcgattc catacagccg agccgcccca catggggcgg ctcctcgttt
     3541 ccccccgtgt ttnnnnnnnn nntgtgagcc gctgtgtgca cggttaggag ccggggtggc
     3601 gtgtgtaccc gccgcagccc cttgaaacga acgttgcggc gtttgtgggc gacgtgtgaa
     3661 agggcccggt gcatcctgct ccgacgatgg gaaaccgagc ctttggggat gggttttccc
     3721 ccccggccat gccccgtttc ggaggttttc gccccttatt tacccgttcg ataaaagcac
     3781 aagataaaga gaggtgcccc taactgggcc cctctc
//
LOCUS       DAWBSB010000215         3805 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_4042_length_3805_cov_2.539467, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000215 DAWBSB010000000
VERSION     DAWBSB010000215.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 3805)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3805)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3805
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4042_length_3805_cov_2.539467"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3497..3506
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          742 a         1169 c         1149 g          735 t
ORIGIN      
        1 gcgttttcag cgttattttc ggttttctgt acggcagcgt gttcggctac gagcatgtgc
       61 tggacccgat gttccacgcg ctgggctttg ctgaaaagcc gctggaagta ctggctcccg
      121 aaggcatcaa caacatcctc atcgccagcg tggtggcggg cgtgctcatc atcgtttccg
      181 ccatcgtcac gggcatcatt tccaatttgc gcaagcacat cattgcaaag accatctttt
      241 ccgtaaacgg cgtttccggg ctggtgttct acgtcagtct catcgcgctt ctgctgccga
      301 tgctgggcat ggagcttccc ttcgtgggca gcgtgccgta tcttatcctg tgcatcgcgg
      361 tgccgttctt cctgatgtac tttgccgagc cgctgagcgc gctgtgcgcc ggcgaaaagc
      421 ccgagggctc ggtgggcgat atccttctca atggtttttt tgagaggttc gacaccgtgc
      481 tgagctttgc ctccaacacc atgagctttc tgcgtgtggg cgggttcgta ttggttcacg
      541 cgggcatgat gaccgtcgtg ttcacgctgg ccaatatgac gggcggtgta ttttatgtgc
      601 tcatcgtcgc cgtaggcaac gtgttcgtca tggcgctgga ggcgctgttc gtaggcattc
      661 aggtgctgcg cctggagttc tatgagatct tcagccgctt tttcgacgcg aacggcgttc
      721 ccttcgcccc gctcagaatc tctctggacc cagccgcgga caaggccgaa tgaacctctg
      781 tgcgcatggg ccgcaccgct gccgtacgca gcttcttttc tgctaccccg cccggacggc
      841 tccggggttt gtgaatagat tttttatagc tttgctgtgc aaggctataa aaaacggtcc
      901 tgtgggccgt cccggcccac ggcctaagat ttcaagcgtc cgggcacaaa gtgcccggat
      961 gcgtgtaaaa aatagtagct gcgctattat tttttatatc aacacaacgc cgctttgcgg
     1021 cagaatggag tatctgttat gaaaaagatg aatgttaaaa agtttgcccg cacctttctg
     1081 gtactgattg ccgtattcgc actggcgtgc gccttttcca gcatggcctt cgccgccgac
     1141 ggcgagaccg cagccgccgc tcccgacaac aatatgggca tcggcatgat cgccgccgcc
     1201 ctggccgtgg gcgtcgccgg catcggcgcc ggcattgccg tcggttccgg cgcgcccgcc
     1261 gccatcggcg ccctcaccga ggacccgaag agtttcggca aagcgctgat cttcgtggtt
     1321 ctgggcgaag gcatcgcgct gtacggcatg ctcatcgcca tcctcatcat cactaagttt
     1381 taagccatga aatatttcct gatcagcgac aacgtggaca cgctggccgg catgcggctg
     1441 gtgggcgtca gaggcgtcgt ggtgcacgaa gaggacgaag tgcgcgcggc gctggacaag
     1501 gcctgtgcgg acccggacat cggccttgtg ctcatcaccg acaagctggt cgcgaaatgc
     1561 tccgcggtcg tatttgaata caagctcacg cgcaagcggc cgctcatcgt cgaaatgccg
     1621 gaccgccaca gcgactcgaa ccccggcgac agcatcaaac gctatatctc cgaggtcgtg
     1681 ggcgttaaaa tttgaggtga cgagcatgaa taagaaagca gccgagcagc agcgcgccgt
     1741 gcgcagcatg gaaacgccgg agcaatactc cgagttcgag gactctgtca ttgaagcggc
     1801 aaaaacgcag gcgcagcgca tcatcgacga cgccaggcgc cagggggacg ccaactttga
     1861 caagctgatg gagggccacc ggggcgaccc gctcatcccc taccgggaag aggccaagaa
     1921 ccgtctggcc cgcaaaatgg cctcggtcaa gcaggacaac cgcaaaaagc tgctggtgta
     1981 ccgcagccag cttgtgaacg gcctgtttgc cgaaatggaa gaagcgctgg aggagtacac
     2041 cgggacaccc gcttatgccc agcgcgtggc ggacgcgctg gccaggcgtg caccggaaat
     2101 ttccggcaaa tgcacggtgc gcctgcgccg tgccgacgag gcgctgctgg cccccgtggt
     2161 gaaaaaggcc ctgccctcgg cggatatcgc cgtggacggt tccatccgtc tgggcggcgt
     2221 caagctggaa acacagcata tcctgtacga cgaaacgctg gatttcgccg ccgcagccga
     2281 gcgcgccgcc tttttgacgc gctgcaagct gcgggtggag taacgcgcgc cgcgcccatc
     2341 cgggcgcgca ggcgcgcaaa cgagtatggg ccgctgccgg aaaagccgtt ttttcaagcg
     2401 ttttttccgg acggcctgga attaggagca agcctatgaa agatgtaatt tattcgatca
     2461 acgggcctgt cgtcacggtg aaggatacca gcacgttcgg catggcggag atggtgtacg
     2521 tcggaaacgc gggcctcatc ggcgaggtca tcagcgtcag cgaagcgcgc accaccatcc
     2581 aggtgtacga gaacaccacc ggcctgctgg tgggtgagcc cgtcaagggc accggcgcgc
     2641 cgctctccgc cacgctcggg cccggcatca tggaaaatat ttttgacggc atcgagcgtc
     2701 cgctcacgga tatcgcccaa aaaaacggcg cgttcatcgc cacgggcgtc catgtggaca
     2761 gcctggatat ggcccgcagg tgggatgtga ccgtcaccgt caagcccggc gacagcgttt
     2821 cgggcggcac cgtcgtcgca acctgcccgg agaccagcat catcacccac aaaagcatgg
     2881 tgcccccgga tatttccggc gtcgttacct gggccgctga aaacggccaa tataccgtga
     2941 cggaccccat ctgtaagata acgacggcct ccggtgagga aaagaccgtt tgtctggcac
     3001 aaaagtggcc catccgcacg ccccgtcccg tggccgcgcg ggagcccatc ggccgtccgc
     3061 tcatcacggg ccagcgcgtc atcgacacgc tgtttcccat tgcaaagggc ggcacggccg
     3121 ccattcccgg cggcttcggc accggcaaga cgatgacgca gcaccagctt gccaagtgga
     3181 gcgacgcgga catcatcatc tatgtgggct gcggcgagcg cggcaacgag atgacgcagg
     3241 tgctgaacga attccgcgag ctgatcgacc ccaagagcgg caagcccatg acggagcgca
     3301 cggtgctcat cgctaatacc agcaatatgc ccgtggccgc acgcgaggcg tccatctaca
     3361 cgggcatcac gctggcggaa tactaccgcg acatgggcta cgacgtggcc atgatggcgg
     3421 attccacgtc ccgctgggca gaagcgctgc gcgagatctc cggccgtctg gaggagatgc
     3481 ccgccgaaga gggcttnnnn nnnnnncctg cccagccgct tggccgcgtt ctatgagcgc
     3541 gcgggctgcg tgcagtccct ttccggcgcg gcgggcagcg tgtccatcat cggcgcggtc
     3601 tcgccccagg gcagcgattt ttcggagccc gtcacccaga acaccaagcg tttcgtgcgc
     3661 tgcttcctgg cgctggacaa aagcctcgcc tacgcgcgcc attaccccgc catcaactgg
     3721 accatgagct acagcgaata ttacgaagac ctggacccct actacaccga gcatctgggt
     3781 gaaaagttct gcaccatgcg cggcc
//
LOCUS       DAWBSB010000216         3799 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_4056_length_3799_cov_2.568643, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000216 DAWBSB010000000
VERSION     DAWBSB010000216.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3799)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4056_length_3799_cov_2.568643"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    282..291
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          708 a         1158 c         1063 g          860 t
ORIGIN      
        1 aaaaacacct ccggcagaac gctcacatcc accagcagaa aacgtcgctc caaatccttc
       61 acctctatcc cttctccatg tacgcaacag atgcacatat gtttttcggt aaagacacta
      121 taacatgttt gcccaccggg cacaagcacc gccgcgggct gtgcgcgtgt tttttcgggt
      181 ttgcacaaaa tcgcctggct gcgccggcaa aaaatcggca gtttttcgca cgccgggcac
      241 gcgcaaagtg tatttcatac cgggacaacc aggtttttct cnnnnnnnnn ncgaaaacac
      301 ccgcacggct ttgccgtgcg ggtgtttatc tatcgcttct gtttttgcgt caggcggcgg
      361 gatcggttgc atggatgccc gtacatacac cgcgctgcac gccaactttg tagtagccgg
      421 tgccgcgggg acacccgtcg gtgggaatat cgcccgagat ggtgcagaac gcgcctatct
      481 ccacgctatc gggcttttcc cactctacaa tttcctcctc tgaaaggtca gcctgtatgg
      541 tgtccatcac ttcagcaaaa gcggaagcgc ccggatggcc cttctggctg cctttgtttt
      601 tgtagatata ataattgttg taatcatcca tcggctcgtt ttcgtcataa ccgtaccagg
      661 tggccatcac atacttgggc gtcaggccca cgaagcacac gtcggtgttg tcgttggtgg
      721 tgcccgtctt gccgatggaa tccatgacgt ttgcatccgg atgaatgccg taggccgtac
      781 caccacggtc gaacaggacg ctgtgaagca gacggttcat gatatacgcg gtgtcctcgc
      841 cgatggcctg cgtggtgaca atgtcctttt ccagtatctc gttgccctga taatcgcgca
      901 ccgaggtgta gctgtgcagg ctcgtcatgc ggccgccgtc gccgtacatc atatatgcgc
      961 cggccagctc atagggcgaa aggccggtgg tggtcgcgcc cagcaccatc ggcgccaggt
     1021 ctacgtccga attctcgtcc agggtcgtga tgcccagcgt gtcccgtgca agctcgaaca
     1081 tcgtgcgcgg cgtcaccata tcgcccacgc gcacggccac cgtattcaga gactgcttga
     1141 gcgcctcgta cacagtcgtc atgctgtggc tgtaccgtcc ggaccagttg ctgggccagt
     1201 cggtgcgggt gccgcccttg ccgtcggaaa tggggaaata atcgtccatc agcacggaag
     1261 aatagttgat caaatccata tcaataccca acgggtaagc cgcgacagcc ttcatagtgg
     1321 aaccgacctg acgctccatc tcggtgccgc ggttgaagcc gcggttttcc gtcttctggc
     1381 caaggccgcc cacgacgccg cacagctccc ccttgtaatt gatgacgacg ccggccgcct
     1441 gcgtggtgat ggtgcgcggg gtatcgtccg ggttattggg gtcgtcgtaa ttttcaatgg
     1501 gcatggactc ccaatacttg ccctccagcc acacgttctc cagtgcggtc tgcacctcgg
     1561 tgtccaccgt cgcatagatg cgcaggccgc cgttgtacag ataatcgctg gccgccttgc
     1621 ggtcccaatt ttccctgcca agcgggtttt ccgccaccag atcgtcgatg acctcgctga
     1681 tgacctgatc ggtgaaccag gagtaatccg tctgcacggc ggcgttttcg tccgtggtct
     1741 tttccaccag attcagcggc gtggcccacg ccgcctccgc ttcagcctgc gtgatatagc
     1801 cctgctgctg catgaacagg atgatgtcgt cgcggcgctt caaatgctcc tccgggttgg
     1861 tataggggtt gaagcgcgaa gggttctttg tgatggccgc aatgctggcc gcctcggcga
     1921 tggtgacgtc cgccgccgat ttaccgaagt actggttcgc gcccgcctcc acgcccgcgg
     1981 tattgcccgt caggccgatg gtgttcaggt acgcttccag aatctcttcc ttgctgaaac
     2041 ggttgtccag cgcgatggcg cggaaaatct ccttgacctt acgggcgtag ccctcgatgc
     2101 cggaggattc atcctcgccg gtgatgtttt tgatgagctg ctgttcaatg gtggacgcac
     2161 ccagctggct gcccgtcagg tagctgcccg tcagcttgcg gaacacctcg ttgatggccg
     2221 ccagcaccgt gcgcttaaag ctgaagcccg gatgcgtgta aaaattctcg tcctccaccg
     2281 cgacgaacgc gtgctggata tacggggata tttcattcag cggcttccag atgcggtttt
     2341 cctccgggct ctgcagacgc tggtactcca cccactcggg ctggccctgg tcgttgatct
     2401 gctggctgta aatgatcgtg gtatacgcca gctcaagctg cgtcaggtcc agcaggtcgc
     2461 tgtcgccctg cgtctctttc accacataca ggctgacagc cactgccgcg acggagccca
     2521 gaatgatgca cacacacagg cagcttgcaa taaagcgcat gatcgcgccc agtactctgc
     2581 ccgccgtcat ttttttcttt ttgttcctgc gcgggccgcc ggagccctgt gcgccccggg
     2641 gcgcgccctg cgccgggcgc tgcgcgccgt ttttattgtt ttctatgatg agtcgcctcc
     2701 caaaagtact tcccgcgcgc cgcatgcgca aagacgggga aaattgtatg ttcccgcatg
     2761 gtgcttacgc catagtagta taccacattt tcggctgcca gacatccgtc catacaggaa
     2821 taggacaaat tgtttgtgcc catactgtgg gcgtgaggtg attgctatga gcgcgaaacg
     2881 aatcgccgtg ctcgtgatcg ttcccattgc tgtttttgcc gtcagcgtgg ggctgtttct
     2941 gctgctggcc catccgggcc gggccggcgc cgccgagcct ccttcctaca cgctgggtga
     3001 ggattccggc aagctggcgc tgttcaaaac cggcgaaaac accccggtga cacggtatga
     3061 gatctacacc gggcttctgc ccgagcagga cgtcgccgcc ctgcagcagg gcatccctgt
     3121 cgccacgcag aaagagctgc gccgctatct ggaggatttc ggcgcgtaac ggcccgcgcc
     3181 gcatgcatgc cgggtcattt ccttgcgggc acgatctcca gccagtagcc gtccgggtcc
     3241 gcgatgaaat agatgcccat ggcctcgttt tcaaagcaga tacagcccat ctccttgtgc
     3301 agagcgtgag cggccgcata atcgtccacg gtgaacgcca gatggaactc gttttcaccc
     3361 aggtcgtagg cttcggcgcg gtccgccagc catgtcagct ccagctggtg gggcgtgacg
     3421 ccgtcgccca ggtaaacgag ggtgaacgca tcagtggcgt ggcgccgcac ctcccgcagg
     3481 cccagcgcct tttcataaaa agccaggctc gcatccaggt cggccacgtt gaaattgttg
     3541 tgtgcgaatg taaactgcat ggtgctcctc ccgccgttca ggctggtaca caaaaataaa
     3601 cgaatcaggc gcgcagaata tttcacgctt cgcgccgctg cgcgcaaaaa tgcacgatgc
     3661 cgagcaaacg cggcacaata cggagttatt ttagcacgcg gcgcgcctgc ttttcaaggc
     3721 tgtaaatgtg aaaaccgcgg cttacgcgcc caaacagcct tttgtcccgc gacaaaaggt
     3781 ttgtttttat gccgttttt
//
LOCUS       DAWBSB010000217         3799 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_4055_length_3799_cov_3.183226, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000217 DAWBSB010000000
VERSION     DAWBSB010000217.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3799)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4055_length_3799_cov_3.183226"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          703 a         1170 c         1273 g          653 t
ORIGIN      
        1 gtttcagagg ctgagttggc gtgataaaat ggatacccgc ttttctcctc ggcccgtagg
       61 gcggggctcc cgcgaaagtt tgcgcagcaa agttttgtgg ggaaaaggag gaatgacgga
      121 gcggtatgag aaaagcccag ccaactggct gggctttttg aatggagcat agtccattcc
      181 gacgtggtgc cggtggtggg acttgaaccc acacggtgtt gccaccaacg gattttgagt
      241 ccgcatcgtc tgccattccg acacaccggc gtacggatag cttttatatt atactatgcc
      301 gcttttgtaa aatcaagcgt tttttgcccg caaaacgcta aaaaaacgat ctccggcctt
      361 ttttcggccc ggcgccgggc cggcctttgg ttccggccgc gcaaagagat tcacggcggg
      421 cgctgccgca gcaagcttcc cattgttaaa aatgcagccg ccggcggaat tcgtttttga
      481 tcgtccggtc gccgaacacg gccatgcctg ccaatgtggc caccgccagg atgctgggca
      541 ccacggcgcc caccaggctg tgcgccagcc cggtgaagta cagcacgggc attgccagcc
      601 caaacaccgc cagcaccgcc tgatacagca cataccccgc ccagttggtg cggttgcaca
      661 tcaccagcac caccaccgcc acgatgcccg cgcagatgaa cgcgggcacc acgaacgaaa
      721 cgccccagcc ccggtagcct gtttcaaagt ccacaagcac ggccagcgcg ctgcccgcca
      781 ccacctgcat gagcaccttg cccgccgcgt tgccgccgcg gcgcatggcg tggggcacca
      841 cggcccaggc gtataccaaa gccgtcacca cgatggtcca ccaccagaac gacggcatca
      901 caagcagatt taccacgatg cacaccgccg cgccgacgac ggagagcagc aacagcaggc
      961 gcttgacaaa attgtattcc ttccggctgc ccgccggcgg atataccgcc gcgcagtccg
     1021 cgtccgccag cccggccagg ggcgtgccgc aaagcgggca cagcaccgcc ggcgtggcaa
     1081 cgtccacatc acacgccctg caatgtttca tccgtatcgc ctcctgtctc cagcgtgacg
     1141 ggcacgcccg cctttgcaag cctgcgcacc acgctgcgcg caaggcggtt gtcctccacc
     1201 tcggtggtga aggtgagcgc cagcgtgttg ccgcaggcgc aggccgtggc cttcaccggc
     1261 tccctgggcg tggtggagag cagaaagcgg aagccctcga aataccggcg gaacggctct
     1321 tccacacgca ccggccccag attcgagaac accagcgtgc tgccattgtt ggaatgctcg
     1381 tacacgatgc ggaggatgcc gtttttcagc ggcaggggaa tgatgcgcgt gatgaggttc
     1441 ttttcgctgc ccgccgtaaa ggccagcttc tgttcaaatt cctcccgcgt cagcttttcg
     1501 gcaaactggc gcttcacggc ctccatcacg tcctcaaagg ggacggcgcg gcgcgccagc
     1561 ggcaggatga tcgtcaggtt ggaaaagaaa ttcagggacg tctcggtgcc gaagataggc
     1621 cgcagattca ccgggacaaa gatgctgacg ggcctttttg cgccgttgca ggccgtgtat
     1681 tcctcgtaca cgccgtaggc gatggcggct gtgaggtatt cgcccacggt ggcgccgaag
     1741 cggcggcact cggccttcag cgcgtccacg ggcatcagcg ccgtggccac gcccagcccg
     1801 cccgcgatgc ggtgctcgcc ccgcagatga tacgcgcccg gttcgcggaa cgttttgctt
     1861 ttggccggaa cgtaatgctt gaggtatccg tcctgcacct cgcccgcggt ctcggtgccg
     1921 tagggggtgg caagctgctc gggcgtgaac gcgtccgggt gggcaagctg gcagtagcgg
     1981 tagcagacgg ccttcaaaaa ctgcatcgcg cccgtgccgt ccgtgagcac gtggaaggtc
     2041 tccagatgca cgcggctgcc gtaatacagc acacggaaca gaaagcggcc tgtctccacg
     2101 gggtcgatat agcggcaggg ggcgtcctgc tcgggcagga tctggggtgt ggcgcggttg
     2161 gtttccagat agttccagaa caggccgtgc cgcaggcgga cgttgaacgc ggcaaaatag
     2221 gggagcgttt gctccagcgc ctgctggagc agcgcagggt ccacagcctc gcgcatcacg
     2281 gctgtcaggc ggaatacgtt ggaatagcgc cggctcgcca ccacgggaaa gatattcgcg
     2341 gtgttgtcca gcttgtgcca ccgcgtgggt gcagcgcgtt ttttcatgga aaagatcggc
     2401 tcctttaaca gagaggatgg gaacgggcag gccgctttgg ggcagggcgg cctgcgggcc
     2461 gcagaaagcg gggaaggggg cggcccgggc cgcatgggag gggacgcggg gaaaatcagg
     2521 cgaaaagtga aaggtcgatg gcctcggcga tggcccgccc gcccgcgaga ttggggtgca
     2581 ggccgtcggg cagggcaaat tcctttttca tacccacgcc gtcgtcggtg tccgcgatgg
     2641 gagcgccgag gtccagcacc gcgtcgaagg ggccttcttc agcgatccag cggttgatgg
     2701 cgcggcgcag cgcctcccgc tcgggcggga acaggtggtt cacctcccgg ggcaggatga
     2761 gccccgcgta tacctttacg ccctgcttgt gcagcgcgtc cgcgatggtg cgggcggcgg
     2821 cggcgtactg gtccacggta ataagctcct cctccggcgc gccgccggtg ccggggtgcc
     2881 ccagatcgtt ggtgccgaga gcgaacaccg cgtgggtgag gccctcgatg ggaagcaggt
     2941 cgtagcggta gcgctcgatg cccgcgtggc cgtacatgcc ccgggaggcg ggggttgcgc
     3001 cgtagagcag gcggttgccg ccgatgccga ggttgcacac gctcacctcg ccgcggaaat
     3061 gctcgtacag gcgcgccgtg aagggcgtta cccacgcgcc ctgctgcatg atggaatccc
     3121 cgaacatggc cacgacacgg ggggcgggca gggcgtcgtc ctgctccacg atcacctcgg
     3181 agagcgtggt ggtggcgctg gagatgtccc agggcagcac gcttttggaa aggtctgtcc
     3241 agaggcgggc cttgggcagc gcgggggcgt cgcagtggtc gcccttgacg gagcggttgg
     3301 caaagatgcc cacaaaattg ccggaggtga ccttttcgcc gccgggataa taaaggctga
     3361 cggcgatggt gcagcccggc tgcacgggca gcgcgaccct gtcggaaaaa acgtcccgct
     3421 ggggcaccag cgcaaagctg gtgtcgccgt gcacggtgag cgggcgcacg gtatcgggca
     3481 caaggcggcc ctttgcgtcc gccaggccga tggtggcgtt gcccacccac acgggaccgt
     3541 cggaataata attgctgaat tttacataga cggcgctgcc ggaaagattg ttgggggcga
     3601 agatgcgcac ggtgcgcgcg gcgttgttaa agggctgcgc ggaaatatcg gtgtgcgcga
     3661 cggcaaaggt gcatacacgg cgcggcatag gcggcctcct tgttattgta taaaatgccc
     3721 cgggcggtgt tgcgcggggc ttgtctccag tataccatgg cgccgcgaaa cggcctcgac
     3781 aaaaccgcac gtgctgcgg
//
LOCUS       DAWBSB010000218         3791 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_4065_length_3791_cov_3.093148, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000218 DAWBSB010000000
VERSION     DAWBSB010000218.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 3791)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3791)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3791
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4065_length_3791_cov_3.093148"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          730 a         1310 c         1011 g          740 t
ORIGIN      
        1 ttatggctat accatatttc caagctcaca cttcgcgtac cgctcgtgtt cactaagaaa
       61 catggtatct acaccgctaa gttcatactt cgcctgcggc tcgcattcgc taaacggaca
      121 tattatacca taaatatggc cgccgcaaaa gagttttacc gcagaaaagc acggcgcgcg
      181 catattttta cgcttcgtcc cgcatgcgcc ctcatattcg gcgcagcagg cacacgctgt
      241 gtgcggcaat gccctcgccg ctgcctgtga agcccagctt ttcctccgtg gtagccttga
      301 cggaaacatc ctctgcggga atgcccagcg ccgccgccag attttcccgc attgcgggga
      361 tatgcggcgc cagctttggc gcctggcaca aaagcgtagc gtcgatgttg cctgccgccc
      421 agcccgcgcg cgccagcaca cggccaacct cctgcagcag cgcaatgctg tccgcgccct
      481 tgtaagcggg gtcggtgtcg gggaacagct ttccgatatc acccagcgcg accgcgccca
      541 gcagcgcgtc catcaccgca tgcgtcagca catccgcgtc cgaatggcca agcagcccct
      601 tttcatacgg caccgtcaca ccgcctaaaa ccagcgcgcg gccttccacc agcctgtgta
      661 cgtcatatcc atgcccgatg cgcatgccgt tccctccgtt tttttccggc gcgggcagat
      721 cctcccgcgt agtgatcttg tagtttgcgt aatcgccctg tgtcagccgc acaggcaggc
      781 ccgccagctc aaaggccgaa cagtcgtccg tcactgcgtc acagcacacc tgcgccagca
      841 cttgccggta cagcgcggtg cgaaaacact gcggcgtctg cacggcgtac agcttttccc
      901 gcgcgggcgt ttccaccacg aggccgtccg ccgccacctt tacggtgtcc ttcaccggga
      961 cggccggcgc agctgcccct gtttcatacg cggcctccag ggcatcccat atcacctgag
     1021 gcgtgacgaa cgggcgcgcc gcgtcgtgga tggccacata ttccgcattc accgcatcca
     1081 ggcccgcacg cacgctctgc acgcgcgtgg cacctccagc tacggtcttt gcgggcttgg
     1141 cacaggccgc ggccagcagc gctgcctgcg gagcgttttc tcccgccacc aataggatct
     1201 cgtctatggc cgggtgcgta tcgaacgcgc ggacgctctt ttccaaaact gtgccgccgt
     1261 ccagcggata ggccagcttg tcgaatccca tccggcggga cgcccccgcc gccacgataa
     1321 ccgccgccac tgtatgccca tgcaccatcg cgctcatctc cttttttaat aatgggaagg
     1381 ctgctgtttt gacggccgcc ctgaatctct gtgccgccgc ctatacccaa cgcccattcc
     1441 ggacgccgca aaggccggtg cctgggcggc gttctcgact ctctgcgccg cctggaccgc
     1501 acgcatgttt tgccgcttca aaagccgcct tctcaagcag cttcatccgg ccttgcttcg
     1561 cggcggtcaa tgcggtcttt catctttttt gtgctcgcgc cgttcctccc acagcatcgc
     1621 cgccgcaagg agcggcccct gtgcaagctg cggcagaatg gcccccagcg cgccctccat
     1681 ggaaaatgcc gctgtccccg caacagcggc caacatcgcc gccacccgca gaagatacca
     1741 gcccgttacc gctgtgccgt ctggcgcggc ggcccatgct ttgcaaaagc aggccagcgc
     1801 cgcggccgaa gccgccacac caatacacca gccggccgca agccagcccc gcgcgccgga
     1861 gcgcgcgtat aatgccgccc ctgccatcag cgccgataca agcgcgttcc acaaaaacgc
     1921 atttcggaac acccgtctgc ggtccatcgc gccgccgcct ttccctgttt cttctgttct
     1981 tcattccaca tggatataac gccgaacatg ccaaaacgtc ttcgctgcat ttttcaaaag
     2041 caaaggctcg cgtcagaaca gagactcctg ctcacagctg tccgcgcccg tgccgcccat
     2101 ggatgcaagc tgctccaaca gcacgccgcg gctgtttggc agcgtcccct cgatgacctt
     2161 ttccaaaagc tcctccagcg cggcgcccac agccacgccc tcgcgaatgc ccgccgccag
     2221 cgcgtctgcg ccgttcacgg ccagcatcga tcggtcgtag cacgcgcccg tagccagcgt
     2281 ccggccgagc gccgcggcaa aatcccgcag gtcatcctgc cgcacgccgt cgccgtgggc
     2341 tacggcccgg gccgcctgca gcagcgtcag gccccgcagc acttccgggc ccagcgtgtg
     2401 cagccaccgc cgaatgacgg gaggcagcgc ctgcagcggc gcgtcggcgt acagcgcaag
     2461 ctgcgccgca atttccgctg tttttgcgtc cgggcacaat attgcggcct gctccggttc
     2521 cacggcacag aacagcaggc ccatacgcac cgtcagatcg tccggcgcgg cctgaatagc
     2581 gcttaccgca ttttcccacg ccgcgtccga cacatgagac agacagggca gcagcggctc
     2641 gaacagcgcg cggtaattgc gcagcacacg cgccacatga cggccgcaga gcattttgca
     2701 gaattccgcc ctgctgcgtt ctatggcgac ctccgtcagt gcgccgcatt tcgccagtgc
     2761 ggcagcggcc gtctgcggct ccacagaaaa gcccatggtg gccgcaaagc gcgccatgcg
     2821 cagcacgcgc agcacgtcct cggagaagcg gcgcgccggt tctcccacgc agcgcaggcg
     2881 ttttgccgca aggtctgccg cgccgccgaa gggatcgatc agcccggctg cgggcgaata
     2941 ggccatggcg ttgacggtaa aatcacgccg ggccaggtcg tcggtgacgc tgcgcacgaa
     3001 tcgcacgctg tccggacggc ggtggtcgga gtacgcgccg tcggtgcgga aagtcgttac
     3061 ctcatagggc gtgtgctcca gcagcaccgt tacggtgccg tggcgtatgc ccggcgccga
     3121 aagacatttc ccgggaaacg ccgctttgat ctccccgggg cgggcggagg tgcacacgtc
     3181 ccagtcgtgg ggcgcgcgcc ccatcaggct gtcccgcacg cagccaccca ccgcaaaggc
     3241 ggaatgcccc tgctgctgca gcgtgcttaa aatgctgcgc accggtgcgg gcagcaaaat
     3301 atccacttcc acgcgcgcgc ctcctttccg tgccgatttc tgcggtaata gtatagcata
     3361 aaatacgcat agaaaaaagg acgcataaaa gaatatccat gtaaaaaagc ttcacctctc
     3421 cccgctttgg gttaaagtga agccttatat tttaaagcta tttttctctt gttaaaattt
     3481 tatcacaagt gcggccaaaa gtcaaagggt cgatttgtgt caagtttttg acttttaccg
     3541 ctatccctca atgttatctc catccgggtc gcaagaacta caataaaaat catatgcact
     3601 ataagtcttg ttctggcaag tgtccccttt ccccatgcac caatattccc cgcctgataa
     3661 tccatcatcc agaacatcgt attcatagtc tggaatgttg tattcatatg ttggcgtata
     3721 gctttttggt tttgtagagg cagaagatga atcattctct ccaataatac ctatcataaa
     3781 gaaaaatagt a
//
LOCUS       DAWBSB010000219         3752 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_4111_length_3752_cov_3.062483, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000219 DAWBSB010000000
VERSION     DAWBSB010000219.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3752)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4111_length_3752_cov_3.062483"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2449..2458
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2974..2983
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          740 a         1076 c         1249 g          667 t
ORIGIN      
        1 catccagcga gtgagcgcgg cggacgttgt tgtgaacggc ggcgcgccgc gcgccattgg
       61 ccgcgggctg atgatccttc tgggcgtgcg ggacaccgac acgctggacg tctgcgaaaa
      121 gctggcgcag aaatgcgcgg gcctgcgtat ctttgaagac gccggcggca agctgaacgt
      181 ttcggccgag gagctgggct gcgaggcgct ggtagtgtcc aatttcacgc tgtacggcga
      241 tacgaaaaag ggcaagagac ccagtttcat cgccgcggca aagccgccgc tttcggtgga
      301 ggcgtatgaa gcattcgtgg ccgagctgcg aaaaacaggc ctggcccgtg tggagacggg
      361 tgaattcggt gccgatatga aggtgacact gaccaacgac ggccctgtga ccatcgagat
      421 cgagaccgac gaatggaagc gctgagtgcg gcgtgccgcc tgaaaaacga aggagaagga
      481 tatgaaaata taccatattc ccgcggcgcc gccgctgtac accaacacgt ttctgctggt
      541 gggggaaaac ggcctggccg ctgccgtcga tccggctgcc ggggcgcagg aatatctgcg
      601 gctgctggag caggagggcg cgcggctgac gcatatcctg ctgacgcacg gccattacga
      661 ccatgtgggc gcagtggagg aactgcagcg gaaaacgggt gcgaaggtct ggctggcccc
      721 gcaggacgcg tgcggcggtg agctttttcc gttcaccacg ccggacaacg cctatgcgga
      781 cggagaggaa atccgcgtgg acacggatct cgcgttccgc gtcatcgcca cgcccggcca
      841 cagcgccggg tccgtctgcc tgctgtgcgg cggtctgctg ttcagcggcg acacgctgtt
      901 cgcgggggac gtgggccgta cggacctgcc cggcggcagt tgggacacac tgcgggaaag
      961 cctgaaaaag ctgtgcgccg cagtgacgga aaacgtgcag gtcctgcccg gccacgagga
     1021 gttttccacc atggaccggg aacgggcgaa caatcggtat ctgcaatttt aacaaaaaga
     1081 taggaattaa tcatatggag atctatatcc ggggccttgc ctccggctac gagcccgagc
     1141 atgtggcgcg gctgtttttt cagcccgcgc tgctggcgaa acggtggccc gcccgcgggg
     1201 cggacgccgt tgcggtgctg cggggcaaaa aacgctttgt atgcggcgtg tgcatgaacg
     1261 gcctgtgcgc cgtgcgcact gcgccgctgg cgcaggacac gccgccggag caggcggaat
     1321 atgagatatg ccgcctgctg tttctgctgc tgtgcgaggc cacgggccgc cgcccggcct
     1381 ggggcatgct gacgggtgtg cggccggtgc ggctggttca cgacatgcgc cgcgccggta
     1441 aggcgcagga agagatagag tcgttctttc gggataaata ctttgcagac ggcgaaaaat
     1501 ataagctggc ggtgcggacg gcggacaatc aggcgcccgt actggcgctg aacacgccgc
     1561 gcagctacag cctgtacatc tccattccgt tctgtccgtc ccgatgcagc tattgcagct
     1621 ttgtttcccg caccactgcg gaatcgggca gcctcattga gccttatgtc caggcgctgt
     1681 gccgcgaact tgcggagatc agcgcgctgg cgcggcgcaa ccggctgcgg ctggagacca
     1741 tctacatcgg cggcggtacg ccgacggcgg tttcggccgc gcagctgcgc acgctgatgc
     1801 aggccgtgcg cgacaatttc gacgtgcccg ctgtacgtga gtataccgtg gaagccggcc
     1861 ggccggactg caccacgccg gagaagctgg ccgtcatcaa agaatacggc gccacgcgca
     1921 tctccatcaa tccgcagaca ttatcggatg aggtgctggc ggccattggc cgcaggcata
     1981 ctgcgcagga tgttctggac tgctttgcac aggcgcggcg gctgggccac gggaacatca
     2041 atatggacct catcgcgggg ctgccgctgg atacgcctgc acggtttgcg cagacgctgc
     2101 agggggtgct ggcgcttgcg ccggagaaca tcacagtgca tacgctcacg ctcaagcgtg
     2161 cctcaaacct cgttatcgag cacgcgccga atgcctatgg ggatgtggca cgcatgctgg
     2221 attcctgcgc gctgctgggg gaaaacggtt atgagccgta ttacctgtac cgccagaagg
     2281 gcacgctgca gaatctgcag aacacgggct acaccaggcc cggctatgcg gggctgtaca
     2341 acgtgtttat tatggaggag gtgcacacca tcctttcggc gggagcgggc ggttccacca
     2401 agctggtggg gccggacggg caactccggc gcatcttcaa ccataaatnn nnnnnnnnga
     2461 atacatcgag cgctttgatg tggtgcaagc gcgcaagcag ggagttgacg acttttatgc
     2521 caaatacgct gatctggatc ccgaaacggc tcgttgaact gggacttgtg aacgtggcgg
     2581 cccgctcgcc cgaggcgttc attgactact gcgagcagga ctatgccgca cgcgtcgagc
     2641 acgcggctgc gtgcgtgcgg gagagcggcg cgcatgtggt gatgctcacc gggccgtctg
     2701 cctccggcaa aacgaccacc gctcacaagc tggcggactg cattgccgcc catgggcggc
     2761 gcagcgcggt ggtgtcgctg gacaattttt acaaggacct gaaggattat ccccgtctgc
     2821 ccgacggcag tgtggattat gagagcgtgg acgcactgga tatctcggct atcaaccgtt
     2881 gtctgctgga gctcatccgc acgggccgaa cacagattcc ggatttcgat ttctgcggag
     2941 agcgccgccg ccacggcgtt atccccgtgg aggnnnnnnn nnntggggga cggtgtggtg
     3001 gtcatcgagg gcatccatgc gctgaacccc cgcctgacgg cgctgctgcc cgagggcagc
     3061 gcgttcaaga tctatgcggg catgcgggag gaatactccc gggacggcca gcgcattctg
     3121 cccacgcggg acgtgcgcct ggcacgccgc atggtgcggg accacaagtt ccgcggccac
     3181 agcttggaaa agactttttc catgtggccc gccgtctgcg agggagagga taagaacatc
     3241 aaggttttca aacccgaagc agacctgctg ctggatacca gcttttccta cgaggtgtgc
     3301 tgtcttgcgc cttttgtcac cgcgctgcgg ggtgagcttg cgccgggaac gccttatgcg
     3361 gcgcagctgg aaagcttgtg cagccgtttt gcactgtgca tgccgctgcc cgaacagagc
     3421 atccccagga cgtccatgct gcgggaattc atcggatgaa tgcattgcaa agcgcgcggc
     3481 ttcatactat actataatca aagctcaagt aaaacaggta aaataagaga ggtgctggaa
     3541 atgaccacgt tcgataaact taagggcgcg gccgccacgc tggcggacgc ggccgtaaag
     3601 acaacaggag acctggccca gaagggcaaa aagcagatgg acctgctggc gctggaaaac
     3661 aagctgagcc gcgcccagcg ccagcttggc gctctggtgt atgcgctgca caaaaacggc
     3721 gaggacaaca cggcgctggt agagcgctac at
//
LOCUS       DAWBSB010000220         3673 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_4209_length_3673_cov_3.445274, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000220 DAWBSB010000000
VERSION     DAWBSB010000220.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 3673)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3673)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3673
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4209_length_3673_cov_3.445274"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          875 a         1066 c         1082 g          650 t
ORIGIN      
        1 gcgccgggat ggtgagctcc acatcgatgt caaaacccag caattccttg aacgcctcac
       61 gcaaaaggtc cttatagcgc gtctccacga tgccctttac aaaatccgag cgcacggaaa
      121 gcgtggccac actgccctca aaggacactg gctcgatgcc gtcgatgaaa agattgtaag
      181 tcgccagcac aaggcgttcc ttgcagtact cctttacagc ggcgaaaacc tcgtcgaatg
      241 aactcatagt atgaaaaaca actcccaaac aattcaatgc aatattgata agattatatc
      301 acattttttc atacagaaca attctttcct ctacattact ttatttacaa tatagaatac
      361 cctgatagcg ttgccgtacg cggtctgttt gaaacatgcg gacggaaaaa accgcgcgtg
      421 tcccccggcg gcggatgcag gcgggtggaa aaaggcagaa aagtgttgac acagagccgc
      481 tttttcggtt atactcttat gttgcaaggc tgtctctgcc agcagagacg gctttctaga
      541 caagagcaga gacggtacta caagatgttg tggttccatc agggaggagc tttttttatg
      601 aaacgtacat tccagcccaa gaagcgccac cgcgccacaa agcacggctt tttggcccgc
      661 agcaagagca gcaacggccg caaggtcctg gccgcccgcc gcgccaaagg ccgcgcgaag
      721 ctggctgcct gagaaggcgg cgctcagcag cgttcgggcc ggcgtttctg ccggcccgcg
      781 caaatgcgtc tgcgtcccgg ccgggatgcg gaaaaaagaa aacggcgcgc cctgcggcgc
      841 cgccgtgaag caaggccact ggcaggtggc ctttttgcgt ataccatgaa atgaaagggc
      901 cttacccatg cgttatcatc ccctgacacg caacaatgag ttcgcgcgcg catacacgcg
      961 cggaaaatcg ttcgtgcacc cgcatgtggt gctgtatgtg aacaaaaatc gtctgggccg
     1021 cacgcgcgtg ggcatcaccg caagcaaaaa aataggcaac gccgtaacgc gcaaccgcgc
     1081 gcgccgcgtt ctgcgggccg cgctgtacga ggtgctgccg ccggacgtgg gcgggtacga
     1141 cctggtgctg gtggcccgcg gccagacacc gcgcctgaaa agcacacagg tggcgaaaac
     1201 actggaaagg cagctgcgcg ccgccggcct tgtgcaggaa tgagtttatg gtgaagcgct
     1261 ttctcatttt tctgataaaa ggctatcaaa aatttatttc gccgcttttg ggtgataact
     1321 gccgttttta tcccacctgc tcggcttatg ccatcgaagc gctgcgggtg cacggcgcgc
     1381 tcaaaggcag ccttttggca acctggcgca ttctgcgctg caaccccttc ggcaggccgg
     1441 ggctggaccc ggttccccca aagggaaaat ggcgcaatga cccgcccact ccgctgcggc
     1501 ccggacaggc tccggctgag gatggtgggg agagggaata aggaatgagg ctgcgccgct
     1561 ttccgcacgg acaccgaaat acgcgccgca tgcgcacggc gcgtcccact tcacataaaa
     1621 aaacgcccgg gcgcagggca gaataggaca gcgaatatga atttcttagg tttcctcgca
     1681 ccccttctcg gcatcattat ggaatggatc tacaagctga tcccgagcta cggctggacg
     1741 ctcatcatct tcacgctgct cacgaagata ctgatgttcc cgctcagcgt caagcagcag
     1801 aagagcacgg cccggatgtc ggcatatcag cccatgatgc aggagataca gaaaaaatgg
     1861 gccaatgaca agaaccgcca gcaggaggaa ctgatgaagt tccagcagga gacgggcatg
     1921 tccatgacgg cgggctgcct gcccatggct gtgaacttcc tggtgctttt cggcctcatc
     1981 gaagttgttt accgtccgct gcagtacatc ctgcgtgtgc caaaggaact gatcgacaaa
     2041 gccgtggagc ttgcgaacag cgcactgggc atgaacctga tggcaaacaa ttttacggtt
     2101 cagaacgaca tcatcaacgc ggtcaaatcc tcgccggacg tgttttccag cgtgttcagc
     2161 gcggaccaga tcgccagcat cagcgatttt aactttatgt tcctgggcat cgacctttcc
     2221 cgggtgcccc attttgcttt tgacgcggcc agcctgatgc tgatgatcat ccccatcctc
     2281 tcggtggtga cgatgatcct ttcccagatc atcaccatga agatgagcgg acaggagatg
     2341 cagggcgcca tgaagtggat gccctggatc atgagcctga tgttcgtgta ctttggcttt
     2401 acggtgccgg tcggcttctc tctgtactac accgcttcca acgttgtgat gctcgtacag
     2461 tccattatcc tgaagaaaat gtacgaccct gaagagatga aaaagcaggt ccaggcggaa
     2521 atagaagcaa aacgcgcgga aaagaagaaa aagaaacagg taaaagtgac cacagacgac
     2581 ggcagggaac aggtaaagga agtaagcgaa gcagagcttg cgcgcatccg ccttgcccgc
     2641 gcccgtgagc tggacgaaga acgctacaaa gacgaataag cccccgggct ttcaaggagg
     2701 gatatttcca tgcgtgaagt gatcaaaaca ggcagaacgg tggaggacgc caccgagctg
     2761 gcgctggccg agttgggcgt aagcgccgaa gaggcgggca ttgaagtgct ggagctgccc
     2821 cagcgcaggc tgttcaagag catcccggcc aaggtgcgcg cctatgtgga ggatgaggaa
     2881 gtccctgagg cgcctgcgcc caaggaagaa aagcctttgc caaaggccgc gccgaaagca
     2941 gccgagttgc cggacgcccc caaggccggg gcccctgccg cgcagccgga gcaagcacag
     3001 cccgctgcag ggcctgttga ggagcccatc gacctggagc agaaccctaa ggcccgcgaa
     3061 gcggtggagt atctgaagga gatctgcgca aaaatgggcg cgcagggcct gaccatcacg
     3121 cccgtcaaac agggcgaggc caccattctg aaggttgagg gcgagagcgc cggtacgctg
     3181 attggccacc ggggcgaagt gatggaagcg ctgagctacc tgacaagcct tgtggcaaac
     3241 cgcgcgggcg gcgactacat gaagatcggt ctggacatca accattaccg ctccaagcgc
     3301 gaggcaaacc tgacagcgct ggcaaagcgc atcggcgcga aggtggcccg cacgggccgc
     3361 ggccacacgc tggagcccat gaatccctat gagcgccgca tcatccattc cgccatcagc
     3421 gagatggagg gcgtaaagag cgagtccatc ggtgagggcg caaaccgccg tgttgttatt
     3481 tccagcacca atcccagcgc ccgtcccccg cgggataacc gcggcggcaa aggcggcaag
     3541 ggcggccgcc ccgcaggacg cggcggccgt gacggcgcgc gcggcccgcg cccgccccgc
     3601 aaagacgggg aatacgcggc ccgcaccagc actccggccc gtgaatttgc ggaccgcccg
     3661 cgcgacccaa gcg
//
LOCUS       DAWBSB010000221         3651 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_4239_length_3651_cov_3.103170, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000221 DAWBSB010000000
VERSION     DAWBSB010000221.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3651)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4239_length_3651_cov_3.103170"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          758 a          992 c         1247 g          654 t
ORIGIN      
        1 atacaccggc gacaccaagg gtgtggcgca gggggtgacg cacgcggccc gcgcgtttat
       61 cccgatgatg gagctgatcg accgggaaaa ggagcaggca cggctggcca aggaaaaggc
      121 ccagtgcgag aaggaaatcg cgtctatggc cgcgaagctg tcaaacgagg gctttgtgaa
      181 caaggcgccc gcgcacatcg tcgaggacat gcgtcaaaag cacgccgcgg cggcggagaa
      241 gctggcgaag atcgaggaat ccatccgaaa cctgggctga gctgcattgg ctgtttggag
      301 aggggaaaga aaaatgagcg gagagttaac gattcgccct gcccacagtg aggatgcggc
      361 cgcgatactg gcgtatctgc ggcaaatcgg cgcagaaagc gacaatctga cgtttggccc
      421 cgagggactg cccatcacgg aggagcaaga acggcaatat ctccggcagg tggagaacga
      481 ccccgacgcg ctgatgctcg tggcgcaaga ggcggacgag atcgtggcgc tgtgcggact
      541 ttcgaggcca cggcggccgc gcatgagcca tcgcgcgcag attgacgcca gcgtgcgcaa
      601 aagccactgg aaccatggga tagccacgcg catgcttgcg gagctgctgg agacgggcgt
      661 gcgcgccggc gtaacagcgt tcacgctgga ggtgcgcgcg gataacgcgg cggccgttca
      721 tgtgtatgaa aagctgggct ttcgcacggt ggggcggctg gaacgattct ttcgcatcaa
      781 cggccaatac cacgccgcct tgttgatgga gctggacgca taggccccgg tataatgtaa
      841 agatacaggg cgggcggcgg cggttgccgt tgcccgccct ctgctgtggg ctttgaagga
      901 gggtggatgc tatggattat gaacaggcgc tggaatgggt acacagcctg ccgcggctgg
      961 ccgcgcatcc gggcgtggaa aatacgcggc ggctgctgga aaagctgggg aatccggaaa
     1021 aaagcctccg gttcgtgcat gttgcgggaa ccaacggcaa gggcagcgca acggtaatgc
     1081 tggcttctgt gctgcacagg gcgggatacc gcgtgggcgc cagcgtcagc ccctatgtgc
     1141 tggaatttcg cgaacgtttt ctgctggacg gagaaatgat ttcccgggaa acgctggcgc
     1201 agatcctgac agaggtgcgc gcggctgctg aagggctgcg cgcggagggc tgggacagcc
     1261 tggtggagtt cgacgcggtg acggcggcca cgctgctgtg gtttgcgcgg gagcgctgtg
     1321 acatcgtctg tctggaagtg ggccttggcg ggcggctgga ttccaccaat gcggtggaaa
     1381 acacgttggt ggcctgtatc atgtgcatcg gaagggacca caccgaactg ctgggcgaca
     1441 cttatgcggc cattgccggg gaaaagtgcg gtatcctcaa aaacaactgc acggtgataa
     1501 gctaccccgc gcagccgcag gaggccatgg atgagatcac gctgcgggcg gcaaaagcgc
     1561 attgtcctct tgtggcgccc gacctgcagg accttcatat ctataaagcc ccggctttcg
     1621 agaaccgggt caactacggc gggtacgacc ttgtcgtacc gttcccgggc gttcatcagg
     1681 cctataacgc cgccgtggtg gtagaggctg cgttggcgct gtgcgaaaaa ggctttgaca
     1741 tttcggacga ggcgattttg gacggaattg cgggcgcgcg ttttccggcg cgcatcgaag
     1801 tattgtcccg caggcctctg gtggtgctgg acggcgcgca taacccggac ggtgcgcggg
     1861 cgctggcggc gacgctgcgc ggcgcagggc tttcggggct gacggcggtg atcggcattc
     1921 tggacggcaa acagccggag gagatgctct ccgcgctcgc cccgtgtttc gcccgcgtgt
     1981 ataccgtccg gcccgacagc ccgcgcgcgc tgtccgccga ggcgctggcg gcgctggcaa
     2041 agcggcattt cccgcaggtg acggcctgtg acagcgtgga acaggcgctg cgcctggcgc
     2101 ggggggacgc gccggagggg cttgtggtgt gcggcagcct gtatcttgcc gcacaggcgc
     2161 gggggctgct cacaaagtaa aacagggcga cgcaaatcaa caaaaaatac aaggcataca
     2221 gtctatgata gatgcataga ctgtatgcct ttcttttttg cgtgccattg cggcacgcgg
     2281 cttcaatatt cagaaaacgg gagacaaaac gggtatgaca caagtaacac tcaagcaaga
     2341 cggaaaaaca cttacggcgc tgctttcggg cgagatcgac catcactggg cggccatcct
     2401 gcgcgaaaaa atcgacgagc gggccgcggc ggccgcgcct gccatcctca tccttgattt
     2461 ttcggcggtc acttttatgg attcctcggg catcgggctg atcctgggcc gccacaagct
     2521 cgtcagcgcc atgggcggcg tggtggtggt acagaaagcg ccgaaggata tcaagcgcat
     2581 gctgtccatc gcgggcatcg aatccaaaga ctaaggccgc gcgccaaaag gagttataac
     2641 aaatgaaagc actgaatacg gtaaagatca cattccccag ccgcagcgtg aacgagggct
     2701 ttgcccgcag cgcgctctcg gcatttgccg cgcaggcgga ccctacgctg gacgagctgg
     2761 ccgacgttaa gacggctgta tccgaggctg taacgaactg catcgtgcac gcctatgcaa
     2821 acaccattgg tccgatcacg ctgacggccg cattgtatga ggacggcacg ctgcgcgtgg
     2881 cggtggcgga caaggggtgc ggtatcccgg atgtctccaa ggcaatggag ccgctgttca
     2941 ctacgggcgg cgccgaacgc gcgggcctgg gctttgcggt gatggaaagc tttatggact
     3001 ccgtcaaggt gcgctctgcc ccgggcaaag gcacgcgcgt tacgctgagc aagcgcctgg
     3061 gcactcgttg agccgctctt tatacatatt atatagagag tagggaagtt atgccgccaa
     3121 caccggtcgt gctcggcccg cgcgccgaat tcattgaaaa gaatctgggg cttgttcatt
     3181 cctgtgccgg ccgtttcaaa gggcggggga ttgaatacga cgatttgttc tccgccggct
     3241 gcatggggct cgtcaaagca tacgacggct ttgacggttc ccgcggcctt tgtttttcca
     3301 cttacgcagt gcctgtgatt ttaggcgaga tcaaaaaact gttccgggac ggcggaaccg
     3361 tgaaggtgag ccgctcgttg aaggagctga gcctgaagat cagcgcggtg cgggagcgct
     3421 gcgtaaagga aacagggcag gaaccgactg tttcacagct ggccgaacgc ctcgatgtaa
     3481 cgcctgaaca agtggcggac gcaatgagcg ccgcgatgcc cgcgctgtct cttacgccgg
     3541 tgagcgacga agacggcagc cgggagttcg atatcccgga ggagagctgc gaggaaaccg
     3601 tatccgactt gctgagcctg aaaagtgtgt tgggcacgct gacaaaggag g
//
LOCUS       DAWBSB010000222         3650 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_4240_length_3650_cov_3.251460, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000222 DAWBSB010000000
VERSION     DAWBSB010000222.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 3650)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3650)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3650
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4240_length_3650_cov_3.251460"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          658 a         1147 c         1154 g          691 t
ORIGIN      
        1 catcaaaagt aaggacaatg cctgacggat aaaaggcaac aaaagacggg ctgccggcag
       61 acgccggcag cccgtctttt gtggatgcca taaaggaaag aaagggcagg cgccttattc
      121 ccgttcaaag gaaagatagc gtgtgccctg aaaggtgagc ctgccgcggt cgccctcggc
      181 cagcatgccg tattccgcgc ccgaaaggcg cagttccatg cggtcgccgc tttccacctg
      241 gaacgtggcg taataagtgg tgctggaggt gacgtgcgcc gcgccgcctg tattgtggtg
      301 ggagtgcgtg gtggcggtgc gcttcgccgc cacgacggcg ggcacggtca ggcgcgggga
      361 atggttgttt tggttccatt gcagcaggcc gcgcactgca gtaacaagga tgacggccaa
      421 aaccgcgaag aatgcgaggg agaacagaat ttcaaatcca ccgccgaaga aaaagaaatc
      481 catcagaaaa aaactccttt cacgtccgag ccgggtggga ggacgtcacg gctcaaagcc
      541 ccaggcgcgc atggcgtcca ggctctcccg catacacagg aaagcatcct tttcccagcg
      601 ctcctgctcg gcaaaggccc agcgcacgcc ggtttcccgg caggcgtcca cggcggcgct
      661 ccagtccgtc acgccctgtc ccacgggcac caacgtttcc gagccgtcgg gggcgatgca
      721 ataatccttg aagtgcacga attccacacg cccggcccag cggcgcagca gtgcgtctgg
      781 cgcgagaccc gctttgccgg agtggtacag gtccagcccg agcgttacgt tttgaggcgt
      841 gtgctccaaa agcagttccg tgcaggaaag gcccggctcg gccgtgtctg cgggcgccca
      901 ttcccgcgcg cgtggatgaa acgccagcgt catgcccagc ggcgcgatgc gcgccgccat
      961 ggcggccagc tccgccgaaa aggcaagcac gctcgcagtg tccggcaccg gagcggggat
     1021 gccgctgacg cagacgcagg tgctgccgca cagcacgttc aagcgcacgg tgcgctcgaa
     1081 atcatcccgt acggctgtgt agaggtcttg cgtggaaacg caggtaagcc cggcgtcccg
     1141 tacggcggcg gcaacaagct ccggctcgaa ctccgggttc caccactgca tctgcaccgt
     1201 gcggtaaccc atgtccgcca gcttttgcag ggatgcgcgc agctcctggg gcgtttgcag
     1261 ataggggcgc agactggaga cctgcgcgcc cacgatgttt tttttattgc tcattacaga
     1321 ttcctcccgg ttcggttttg agaaaaaagc cccgctgtgc gcggggcttt tgcgtgcggt
     1381 ccacacccgc cgcgcggccc tgtgcaaacg gagccgcgcg ttattgtttt atacggggtg
     1441 aaaaggcgct ttattacagg gttgtcaaaa caagctgtgc gcggcccgcg atggtatacg
     1501 cgcccaagcc tgcgggaagg aacaggctgt cgcccttggc aaaaggcaca gccccgccgc
     1561 cgcaaatgac tgcgccgctg ccggacaggc acagcaggga atggaagctc tcgccgtccg
     1621 cgcacagcgg cgcgctgccc gctacgtcca gcaggcgcgc ggtgaaataa ccgcacttgg
     1681 ccagcagcgt ggaagtgcag tccccgttct gttccggttt ccccatgggg cctgccggac
     1741 gctcgggcgg gcacaggcgt gccacgtcca gggccttttc cacgtgcagt gcgcggggct
     1801 ttccgtccgc gcccagacgg ccgtaatcga acacgcggta ggtggtgttg gaattctgct
     1861 ggatctcggc gataaggatg tccgcgccga tggcgtgcac ggtaccggcg tcaatgaaaa
     1921 atacgtcgcc cgcttttacg ggcacggcgt tcagtacatc ggtgagcgtg ccgtctgcga
     1981 tgcgccgggc ggcctcctca cgggtgagtt cccgctggaa gccgtaatag agctgtgcgc
     2041 cgggctgtgc atccaccaca taccacatct cggtcttgcc cggttcgccc tccacgcgct
     2101 gggcgtaggc gtcgtccgga tgcacctgca cagagagcgg cctggccgcg tcgatgagct
     2161 tgatgagcac ggggaacacg ccgtctgtat gccttgtccc cacacatgcg gggtgccggg
     2221 ccgcatattc cgccagcgtc cggccggcgt ccggcccatt gacaatggtg ctggggccgt
     2281 ccttgtggca gctcagctcc cagctttcgg ccaggggcga cagtctggta tgcttgccgt
     2341 attcgctttt caggcgttca ccgccccaca ggtagtcctt gaacgcgggc gcgagcttga
     2401 gcggcattgc ggccggggcg gaaagacaca gcgcagccgc gccgatgatg cccgccttgt
     2461 tgcccagcgt ggcggtgtcc acacgtgtga caaagctctt gtcaccgccg aagcaatggg
     2521 ctttcacgta ttcgttcatc gggtccgtca gggctttgcc ttcgccggag ataccgccgc
     2581 ccagcagcag catttcaggc cgaaagatat tcaccatgtc cacaatggcc tcgccgagat
     2641 attcctcgta ctgcgccacg acggcctttg cggcatcgtc gcccgctcgc atggcgtcgt
     2701 atacggattt tgcggtgatg cgttctttgt tcagcaggct ttcgggatgc gcggccgcag
     2761 cctctgaagc ctggcggatg agagccgtgg cggaggcata gctttcgatg cagcccctgc
     2821 gcccgcaggt gcattccacg cccccggaga tgagcgtgca gtggcccagc tctgcgcccg
     2881 cgctggcctg cccctcatag atcttcccgt cgatgacaac gccgccgccc acgcctgttc
     2941 ccagcgtgag cagtacggct gttttgcagc ctttggccgc gccggcgacc acctcgccca
     3001 gggcagcgca gttggcgtcg ttgctcaggc ggcagggcag gcccgtcagg cgcgtcaggg
     3061 tggggcaaag gggcacgtcg tcccaataca ggttgttgga atagacgacc tcgcctgtgt
     3121 cgccgttgca gatgcccggg ctgcccacgc cgatgcccgc acagtcggcc ctggaaaggc
     3181 ccgccttttc cacaagttcc atcgccaggc gggccatatc ggccgcgacc tcctgccacg
     3241 ggcgctgcgc ctttgtggga acgctgccct tttccaacag cttatagtcc tctgtaacga
     3301 tgcctgcggc gatatttgta ccgcccagat caatgccgat cgcataacgc attgtagtga
     3361 tgtccccctt gttttccgcg ctggtcacac gcgcgtttta ctactgtaaa gtgtaaggca
     3421 ggggccgggg catgtcaaga gaacacgcgc gttttagacg gaaattttct agtggcgtac
     3481 aggctgggaa gatggtataa taaccttaaa cccgccgctg tcgcggctgg gcgtgtgcgc
     3541 tgcacacgcc cctgtgccgg cgggcatggg cttaaaaata ttgaatcgcg caaactgtgc
     3601 cgagcgcagt ttttattata gccgcctttc ggcagcgtcc tagtatgatt
//
LOCUS       DAWBSB010000223         3602 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_4303_length_3602_cov_2.525233, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000223 DAWBSB010000000
VERSION     DAWBSB010000223.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3602)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4303_length_3602_cov_2.525233"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2547..2556
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          685 a         1174 c          962 g          771 t
ORIGIN      
        1 cccccgttcc tcccaccaca gcagcagcgt caccacgaca aaaatcgaca ccgcgacggc
       61 cgacgccaca cacaggcgga agaattccac gatctcggca tagcgccaca ggctgtcgta
      121 catgcccatc aggccgtaga cgatctcata gcagcataca aagaaaaagc agctggaaat
      181 cagcaggctg ttgtattcgg aaaacgaggt gcgtcccgtg atgagcgccc atggcagcat
      241 atacgacacc gtcaccacaa cgacgtcgaa cgcgatcaat atctgctttc ggaaccgctg
      301 tgcaaaggcg gaaatatggc tcagcaaaat ttcatcacca atctttattt acatcctgcc
      361 cttttgcagg cccggcggta cgcctgcaaa gcgcggcgtc cggacggccc tgtgcggggc
      421 ggcgcgcgga aacagcctcc gcgccgcttg attctttcct attttacacc attttctctc
      481 aataatcaat gttggcataa aatgcgcgcc gcaaacgccg gaaagggccg cgccataccc
      541 ggcgcggccc tttggaaaat attatagaat aatgcagata agccccgaac agccttcgtt
      601 gataacgcgc tcaatggtct cctgcaggcg tgtgcgcgct tcggagggca tatgcagcag
      661 cttgttctgc agcccctcgt tgacaagctc atgcaggctc ttgccgaaga tattgctctg
      721 ccatatcttg cccgggtcct cctcgaaatc agcaagcagg ctcttgacaa gctcttccga
      781 ctggcgttcg ctgcccacga tgggcgcgac ctctgtggat atctcggtcc gtatcatatt
      841 gatggacggc gcgctggccc gcaggcgtac gccgtatcgc ccgccctgat ggacgatctc
      901 cggctcttcc agcgtcagct cgtcgatgcc cggcatcacg atgccatagc ccgtggcgtt
      961 cacctcgtcc agcgcgcttt tgatcttttc gtactgcgtt ttgatgcgcg ccagattgat
     1021 gatgcacggc atgaggcttg cttcgtcgca tatctcaagc cccgttgtct ctcccaacac
     1081 cttgtaaaag atatcgtcct gcagctccag cgccatagtg gcgcagcccg tgcccaggtc
     1141 tatgaacttc atcttgctgg tcttgacata tggacactcc acggtccgcg gcgaggatgc
     1201 gatgtccttc atcacgctgg cgcccgccgc aaactcctgt accgctgtat atacagcctc
     1261 ctgcagccaa tgtccgtgat ccagcatcgt gacccatttg ggcatcgaaa acgcgatctc
     1321 cttgagcggg aactcaaaca gcacgttctt gaggatctcg gtgatggtct cctccgtcag
     1381 ctccacacag ctcacgggca tgacggtgcg gccgtatttt ttgctcagct cctcggccag
     1441 agtcatgctc tccagcgact ccggcgcctg aatgacgcgc tcctcggcct tctcgtagcc
     1501 gctgcgtggg atatcgctga tggaaccgtc cgtggtgacg acaaggccga tggtggaatg
     1561 ctcacagatg acctttctcg tgccggtttc cgccgctaca tcgaaaggga cctcttcgtc
     1621 gaaccagggg cttttcacca tgcggggctt ttcattttcc tcgtgcccca tggcgccggg
     1681 caccatgtag cccacgcagt cgatgagacg tgtgcggaag gaggccccgt cctcaaggtc
     1741 gatggagacc gcctgttcgg gcacgaattt cggctcggtc gtcatgatgg ttcgcccggc
     1801 ggcggattgc ggcagttcgt cgcgcgcccg ctggctgaac gcctcgctct ggatctcggg
     1861 cagcaccatc agctccataa agcgtttgat gaaagtgctt ttgcccgtgc gcaccggccc
     1921 gacgacgccg atgtagatgt cgccgcctgt acgagtggcg atgtctgtat aaagattgca
     1981 atgatccatc ctgttttctc ccctctcctc aagtagccga cggtatcagc aggcgctgcg
     2041 gcgcttccag gatgcccgag ccggtatcgt tcgcggccgc aatagcctcc ggcgaagccg
     2101 cataccgctt tgcgatatcg aacatatcct cgcctgcctg tgcaaaatag atgcgcagag
     2161 cgatatcccc gtcgtcccgc accagcggcg acgtgcactg cacgtcggcc agcaccgtct
     2221 gtgtggagcg gaagctgacg atgccccgca ccgtcaaaac aatagccgcg ctggtgtcgt
     2281 cccccacctt gcgggccgaa acgctgccca ccgacggata gcactgggcg atcacgtccg
     2341 ccgcaggccg cggataacgg cgcggcagcg tatattcaca caccttgtcg tagcagtcta
     2401 tctcctccag cgcattgatg cacagcagat gtgccatcac acggccgcgc agcaccgcct
     2461 cgccgtcctg ttcgatgacc tccataggca gcggcgtcgc catcacatca atgatgcgcg
     2521 cgttctcgtc cggcagttgg cctgtgnnnn nnnnnnctcg ctttcggtgt aaaacgcgtc
     2581 gcttaccgcc atgtattcca ccgggcggta caccttgacg tacagcgccg ccgtggcgga
     2641 gatggtgttc tcaccctcat cctcgccgga gcctcccgtg atggtgcagc ctgtgggctg
     2701 caccactgca aaacactggc agtcctcggc cgcgccctcc acatccagta tctcattgaa
     2761 aggtatctgc cgcgtggtgt gtaccagtgt gtggccggga gccgtgcggt ataccacctc
     2821 cgcgcggatc tcgcctttga gcaccgcctt tccggaaacg agctttacct cgttcactgc
     2881 gccggtacac tgggtactga gcaccatctc cggaggcgcg tcaaacgcaa tgctttcctc
     2941 agccgtgata agtttttcct gcgatcccac cgtcttgctg cccgtgagcg acgttgtgcg
     3001 ctgctggatg ccgccgccgg ccagcgcggt aatgacctcc ccctgtgcct cggccacagc
     3061 ctcgatggac agcgcgaacg cgccccggac gtccacgcgg cgctggttga ccgcccggca
     3121 gttcacatac tccgtctctc ccgagacaag cacggtcgcc gggccccagc ttcccggctt
     3181 cacatccacc tgttttgtga acggcagctt ttgttccgcc tgacacagac tttcgtcccc
     3241 ttcggactgg taatatacca cgcaccgcaa ataaccgtct accgtgagcc tgcccgccgt
     3301 gacctgcttt tgcagcacca caagataaac aaagcacttg acgatcttga acacctgcgg
     3361 caggtagtcc gggatgagca tttcggtctc caccgggagt tccagttttg tatcgcagat
     3421 gctttccgcc gcggtgacgg tatctttgta cacctttaat tccatacggc ctcgtcgccc
     3481 ctttcgctgt tctaactcat ctatatgaag cggtgcggcg atgtatgcga aaaaaacaaa
     3541 aacgcccggt gcgcggcaca gcggaaagca tgcgcttttc cgcggccgcc caccgggcgt
     3601 tt
//
LOCUS       DAWBSB010000224         3595 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_4311_length_3595_cov_3.301977, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000224 DAWBSB010000000
VERSION     DAWBSB010000224.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 3595)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3595)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3595
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4311_length_3595_cov_3.301977"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          763 a          988 c         1150 g          694 t
ORIGIN      
        1 gttccgggct tttgccgttc ccatcgcgga tagccggcgc cgcactccca aagcaggcgc
       61 gctaccaact gcgctacacc ccggtatgcg gttttggcac ggatgtctgc gtgccagcgc
      121 ggcggatacg gcgcttacac gaggaatcca agctaaaata ttatatgaca caatgcgcgc
      181 gaagtcaagc caaacccttg acgaaccgcg ccgcctgccg tacaataaac ccatgtctta
      241 ttgttcctat catttataag aaaaggtgtg tcacgatgga aaacagcgca aagaccatgg
      301 accagatcgt cgccctgtgc agaaaccggg gctttgtata tccgggcagc gagatctacg
      361 gcggcctggc caacagctgg gattacggcc ctcttggcgt cgagtttaaa aataatgtaa
      421 aaaaggcctg gtggaagaag ttcgtgcagg aatccaaata caatgttggc ttggattccg
      481 ccattctgat gaacccgcag acctgggtca cctccggcca tgtgggcggc ttttccgacc
      541 cgctgatgga ctgccgggac tgtcatgccc gtcaccgcgc ggacaagctc atcgaggacg
      601 cgggcggaca cgccgagggc atgagcttcg acgagatgac ggcatatatc cgcgagcatg
      661 gcatcgcctg cccggaatgc ggcagcaaaa attttaccga catccgcaag ttcaacctga
      721 tgttcaagac gtttatgggc gttaccgaag acgccaagaa tgaggtctat ctgcggcctg
      781 aaacggagca gggcatcttt gtcaactacg ccaatatcca gcgcacaacg cgccgcaagc
      841 tgccgttcgg cgtgtgccag atcggcaaat ccttccgcaa cgagatcacg ccgggcaact
      901 ttattttccg catccgggag ttcgagcaga tggagtgcga gttcttctgc aagccggaca
      961 ccgatctgga ctggttctac tattggaagg attactgcaa gaacttcctg ctgagcctgg
     1021 gcatgaaaga ggaaaacatg cgcctgcgcg accatgagaa ggaggagctg tccttctatt
     1081 ccaaggctac gacggacatt gagtatctgt tcccgtttgg ctggggcgag ctgtggggca
     1141 ttgccgaccg caccaattac gatctgggcc gtcatcagga ggccagcggc aagagtcttg
     1201 aatactttga cccggagacc aatgaacgct atatccccta tgttgtggag ccgtccctgg
     1261 gcgcggaccg tgtggcgctg gcgttccttt gcgaggccta cgacgaggaa gtggtggacg
     1321 ccgggaaaaa cgacacccgc gtggtgatgc gcctgcatcc ggcgctggcg cccttcaagg
     1381 cgtgcgtgct tccgctttcg aaaaagctgg cccccaaggc cgaggaagtg cgcgccgagc
     1441 tggcgaagta tttcatggtg gattatgatg agaccggctc catcggaaag cgttaccgcc
     1501 gccaggatga gatcggcacg ccgctgtgca tcaccatcga ttttgagacc gtgggcgacg
     1561 acaagacggc cgccgacggc tgtgtgaccg tgcgcgaccg cgacaccatg cagcaggagc
     1621 gtgtgaagat cgaggacctg cgcgcttata tcgagaaaaa gatcgagttt taaaaccaca
     1681 tgcaaagcgg acgtcccagg ggcgtccgct tccttttttt gcctgcttgt ggtatgatga
     1741 aaacaagcca tatccaacag aacggaggag tttttatgat cggctggctc atacttgcgg
     1801 ctgtgctggc gttgtgcgcc gtgttgtgca tccgtgcggc ccggttcaaa ccggaggcgg
     1861 gggctggggc ggcgcccacg cccgtcgccg tggacacaga gggggctgta cagcgcctgg
     1921 cgcagctggt gcgcatcccg acggtgtcca attatgatgc ggaaaagttt gatgaaacac
     1981 agtttgaagg cttccgtgag aagctgcggg aattgtaccc ccgcgtacat gcgatgtgcc
     2041 cgcccgtgcg catcgcccac acgggcatgc tgtttcactg gaaaggaaaa agcagcgccg
     2101 ctcccgttgt cctgatggcg cactatgacg tagtgcctgt agacgaagcg gggtggaagc
     2161 atccgccttt ctgcggcgag gtgtttgacg gcgagctgtg ggggcgcggt acgctggaca
     2221 cgaaaatcac gctgctgggc attctggagg cggccgaacg gctgatgagc gagggctttg
     2281 tgccccggaa cgacgtgtac ttttctttct caggcgacga ggaagtgagc gggccgagcg
     2341 caccggccat cgtggaatat ttgaaggagc ggggcgtcag gcccgcaatg gtggtggatg
     2401 aaggcggcgc cgttgtagac ggcgtattcc ccggcgtcaa gcagcccatc gcggtggtgg
     2461 gcatcggaga aaaaggcttt atgaatgtgg agctgacggc gcgtgccgcc ggcggccatg
     2521 ccagcacgcc ggccgtgccc tccacgctgg gcatgctgtg ccgggccgtg gccgattgcg
     2581 aaaaacacca gttcaaggcg catctgacgg cgcccgtgcg cgcgctgttc caaaacgtgg
     2641 ggccgtatgc gcctttcggc ctgcggctgg tatttgcgaa tctgtggctg tttgggcctc
     2701 tgctgccgct tctggcgggc aggctgggcg gcgagctggg cgcgatgatg cgcaccacca
     2761 tggcgttcac cacggcgcag ggttccaagc agatcaacgt gctgcccaca gaggcgtcgg
     2821 cgggcgtcaa cctgcggctt gtcaatctgg atacgccgga aagtgcggcg cagcacctga
     2881 aagacgtcat tcgcaacgat aaggtggagg tcaaggtaac atacgctcag aatgccagcc
     2941 cgtatgcctc cgcggaggat gcgaactggg agacgctggc caaggcagtg ggcgacacat
     3001 ggcagggcag catcgtctcg ccgtacctga tgatggcctg ctccgacagc cgtcatttca
     3061 gcgccatctg ccgggacgta tacaagttca gcgccatggc gctctccaaa gagcagcggg
     3121 ggctgatcca caacaacgac gagcgtatcc cggtgcgtga gattgccaag acggtggaat
     3181 ttttcacccg gctggagcaa agcatataac gaagcagacc gccgcgcggc gcgcggaaat
     3241 cctgatgatt tggagaaaat gaaaatggat atgaatttgg tgaagcagta tgcggaattt
     3301 gccgtaaaga tcggcgtcaa cccccagccg gggcagacgc tcatcatccg tgcgcccatc
     3361 gagggggcgt ttttcgcccg ggcctgtgcc gaggcggcct acgctgcggg cgcgaaggaa
     3421 gtggtggtgc actattcgga cgaaaagctg tcccgcatta aaatggagca taccgccgtg
     3481 gaggtattgg aggatgtgaa gccctggctc cagcgcagct atctcgatta tgtggagagt
     3541 gagggaggcg cgtgcatgct gtccatttcc gcgcgggacc cggagatcta caagg
//
LOCUS       DAWBSB010000225         3593 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_4313_length_3593_cov_2.722442, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000225 DAWBSB010000000
VERSION     DAWBSB010000225.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3593)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4313_length_3593_cov_2.722442"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1185..1194
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1461..1470
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          730 a         1127 c          936 g          780 t
ORIGIN      
        1 gaccaaatct gtacgtcctg tacagtggac gccgtcggca catcactgcg caccgtgtcc
       61 gcggtcaagg cttgcagctc gtccgtgaga taatatttca ggttttctgt ccgccgctcg
      121 atcgaatccg cagactgttc ccacgagtaa tacaccttgg cgaaattcgt cacaaagctc
      181 tctatcgcat tcgtgtccgt aacttcctcc tgcaccactg tcgtttcatg aacggtgtgc
      241 gtatccaccg ctgtaaaatc cttatacacc gcgaaagcaa acgcgcagat gagcagcgcc
      301 cacagcgcga tcacaaccgg ccgccgcgtc ccaatgctga ggacacgcgg ctttttctgt
      361 tttccctgat ttttgtttct attctggaac atatcgtttc cctgcctttc tgtacttcac
      421 tgtcctaccc gtccggcaca gaccagatgc gcctgccaat aggcgctggt caaatctgca
      481 aacccgatcg ggtttcctgc gtggtacatc cggttatcgc ccacataaat cccaacgtgc
      541 gtgatataat cgccgcagtt atacgtcccc tgaaagaaca ccagatcgcc gggctgggcg
      601 tccccgaacg ggatgtgccg ggtcgcgtcg tactgcgtct gtgcggtgcg cggcaaggtg
      661 atacccgcca cgccgtagca ccattgcgtc aggccggagc agtcaaagga tgttgtcggg
      721 gacgcgccgc cgtagacata ttcccagccc tggtatttca gtgcttcatt catgatcgca
      781 tttacagtct cgctatcaaa ctgtgcggca tacaaatact ccgataccag ccggacatag
      841 aacatattgc cgtagttgta gcgccagccg ccgttttccg cgatggatat ttcattcttg
      901 taggtaacac gcaccccgcc ggacttttcc tctgcaaacg cctgcgccag ctcgaagctg
      961 tacttgccgc cgtgcgacgc gacataatcc agaaacccgc cgccatagtt gtacgcctgt
     1021 atcacgctgt ccagatcgca gccgagccgg tccgccttgt cgatcagttc cgagaaatac
     1081 ttgcagccct gtgcaatgga ttgctccgtg ccgagcgagt tcggcggcag gcccatagat
     1141 tccgaggatt gcatcacgtc ctccagcgtg ccgccggatt ccacnnnnnn nnnnatagta
     1201 aaggccgccc ctgccgccgt cctcgtctga tggaaacagg agggcaacca gcaggagcag
     1261 gcagaagaag acggcaaaca gccctgcaag gggcagcgca agatatttca gcttcattta
     1321 ctgttgcccc cattctgctt gccgcccacg ttgccagtat gccccatgcc atcgggcttg
     1381 ttccgcatcg tcaccggctg tttgtgctgc accgcacgtt tatcggcagg tttgggaacc
     1441 tgatttttca tttccggcac nnnnnnnnnn caccttgggc gcggctgtca cgacagggcg
     1501 caccgtatcc tgtttgccct gtgcttgcgc agcatttttc gcctgtactg ctgccggacg
     1561 gtctaccgca tccggcctgc ggctttccgc gtggacggac tgttttgccg atgcgacatt
     1621 ctgcctgtcc ggttgcttca tacgttcgcc tgcatccgtc tgagcgaccg ccggacgttc
     1681 atgcggctgg gctgccggtt tgcgtgctgt gccgccggaa acctgtgcca taccaacagc
     1741 atccaccgcc gcaggccttc cctgtctgcc tgagacaagc tgttgcttgg gcttaacgtc
     1801 ctgcgctttc tccgcctttg gtgcaccggc cgcaatgacc ggcctttcat gtgcctgctg
     1861 gattgcagaa tattttgttt ccacctgttc cgctgccgtt accggacgtt ttgtttcctc
     1921 ctgtgcagat gccgttgccg gacggctgcg cgccgtgctg ctgctcttgg cagtccccgc
     1981 agtaggcttg tccgactggg cgggaagtac agccgccgga gcatcataca tgccatcatg
     2041 ggacggcggg ttaccccctt gtggcggtgc gccggtgccg cttcccgaac cgccgctggg
     2101 taagcccctt acaggcactt ccggcaagcc gtgtaccgca cccgcctgtg atgccccgct
     2161 gctcccaccg tccggccggt tgctgcgata ccggtatccg ctgtccgctg tgcctgccgt
     2221 actgctggaa cggctgttct gcgtactgcc gccgtcattc ggtctgctgg ccgtcgccgc
     2281 gcgcatcgtc cggttgagcc tgtggagctg atgatgcagg aacatccgcg ggcgatgcag
     2341 gatataccgt cccatgccgc gcgcaccgtc gtcatggagg gaaaacatgc tgagcaggtc
     2401 gcccagcttg aagtaaatcc ccgcgaacac gaccacctgc aaaaacgcaa tgataaagaa
     2461 cggatagccg ctggaaatac ggtacagcat ggtagaaatg ctgaaagcca ccgtgataac
     2521 aagcgtaata cccgcccgcg ccaagatcgt attgaacagc ttgatcaccg cgcgcctgct
     2581 gctgccctca aatgtcggca ccatgctgag cacaaacgac acaggcagga acatggcata
     2641 gatgatgaaa agcacctgtg agaagatcat catgccggta agcagaaaca cgaaaaccga
     2701 gatgccgaga ttgaacagga acagaaaaaa caccgtgccg agccggttca ctgttttcgt
     2761 gaccgtgagg ttggtgttcc cgttgtcctc gatctcggcc ttgacggcat tttcccgatc
     2821 ttcgccgttg ttcccgccgg ggctggccga aagcaggctt tcaacacggt ctgcaccgat
     2881 cgcgtccgtg tccgtatccc cgaattgcag gagcagccac ggctgcttga cctgaatgga
     2941 aaacaggctg tcccgtatca aatccacgct gtccgcaccg ctgctctcca catccggcag
     3001 gacgatcttt gtccccacgg acaaactcgc tgtgctgacg tcctcggaaa actcgttcag
     3061 gcgcttcacg cagtccggcg cataggcgat cagcgcaccg gacagcacga agatcagcac
     3121 catgttcatc accgcacgca ccgcttttgt cgtctcgcgc ttgataagcc ccgtatatgc
     3181 cacatacgta ccaacaatca ggatgataag caggaggaag cccgcataaa acccatccgc
     3241 agaaagcccg ctgggggaga tgcccgcgat agactgaatg ttccgtccaa tctcgtccgc
     3301 tgcatccgaa ataaagtcca gcccgtatgc ttcctgtacc agatagccgg acgcattgga
     3361 aagatataaa ttgattgtcc agataaaatt cgtgatgcag tacaggccgt acatgacctg
     3421 cttgccgatg ccgtctatcc agttccacgg cagccaatcc cacccgttgt caacgtagaa
     3481 gtcaagctgg taattttcca gcgggtagcg gctgtactcg tgcgccgcat ccacactatc
     3541 atccaccagc cccaccgcgc ccgcagacgg gcaaacagaa gtaaacagca aaa
//
LOCUS       DAWBSB010000226         3562 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_4353_length_3562_cov_1.951811, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000226 DAWBSB010000000
VERSION     DAWBSB010000226.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4353_length_3562_cov_1.951811"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1157..1166
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          634 a         1148 c          940 g          830 t
ORIGIN      
        1 cttgttaccc tctaccacat agaacttgtt catgtattcg taggccttga tggggtcccg
       61 gatgcccacg gacaagtgcg cgtcgcacag cgcgttccat ttctgggcaa attcagtgtc
      121 ctcatccaga agcggcatga aattacctgc aaatgccgtt ttgcgcccgg ccgttttcgt
      181 accgacgatg gatgccaaag ggatatttac aacgccgagc ggttctgtat attccaccgc
      241 ggtgtgctcc aggatctcgt ccagtgcctg caaatacggg taagtccctt tcagcacgct
      301 cgaccggaac tctttctgtc caaggcggag cgccttgcgg taatcttcct gtgccatatc
      361 cattgcctcc tcttgtgctg gtctcttttc tttatattat cctttttgat gtgggaatgc
      421 aattatttcc tcttacacct ggccgcaata ctggttgccg ggcacaggcc cactctgcga
      481 aaaggagcct tctgcaatgt tcaaactgga aacacactgc cacacctctg aagtgagtgg
      541 ctgcagccgc ctgcccgccg tgcagctcgt cacacagctc aaacgctccg gttacggcgg
      601 cgccgttatt acagaccact tccaccatgg ctatttcgac agccttacag gaagctggac
      661 acaaaagatc gactgctttc tgtccggcta ccgcgccgcg cgtgaagcag gggcacaggc
      721 cggcctgcac atttactgcg ggcttgagtt tcgcaccacc acaagcgaca acgactatct
      781 cctgttcggc gctgcgcccg aactgcttta cagtacgccg gagctgcacc ggtataccat
      841 cccgcagctg cgcgccctgg cggaccgcga aggcctgctg ctggtgcagg cacacccgtt
      901 tcgcaccggg ctgacgcggg aggaccccgc cctgttggac ggcgtggagg tgttcaacgg
      961 caacgcccgt catgactctc acaacgatga cgcgctccgc ttcgcccgcg aaaacggcct
     1021 gttgtttttt tccggctccg atcatcacga gaccgaggac cgtggacgcg gcggcatggt
     1081 gctgccgcgc gagccgaaag atgaagcaga tctcgtccgc ctgctgcctt cagcaggcct
     1141 gctgcccgat atccttnnnn nnnnnntttt tcccggtcaa gcccgaaaaa taacatctga
     1201 aaaacggccc cgccgctggc attccagcag cagggccgct tttttgtatc attctccggc
     1261 gcggcgctca gcaatgcagt acgatatccc cgtcctgcgt atccacctca atggccgtca
     1321 gcggctcgcc gctgatgatc ttcccggcga tggcatcctc cacctctttg cggatgacac
     1381 gccgcaggtc gcgggcgccg aacttgccgc ccttcgcctt ttcacacagc agggcaagcg
     1441 cggcttctgt ccagctcaga ccgatgcctt ttgccgcgag aggttccttg tactcgccaa
     1501 gcatcagagc ggcgatccgc ttcaggctct ccggagagag cggcgcaaaa gtgacgatct
     1561 cgtccacacg gctcataaac tccggccgca gaaattcgcc cagcgccttg atggacttat
     1621 ccctgctcat ttgagcgatg gtacggttga agcctgtttc tccggtcttg tcgccggagc
     1681 ctgcattgct cgtcatgcag atgaccgtat tttcaaaatt gaccgtgcgg ccctgggcgt
     1741 cgttgatttt tccctcgtcc aggatctgaa gcagcaggtt catcacatcc ggatgcgctt
     1801 tctcgatctc gtcaaacaac accacgctgt atggcttgcg gcgcactttc tccgtcagct
     1861 gcccggcctc gtcatagccc acatagcccg gcggcgaacc gatcaggcgg ctgaccgtgt
     1921 gcttttccat gaattccgac atatccagac ggataagcgg gtcgaccgta tcgaacagct
     1981 gcagcgcgag ctgcttgaca agctctgttt tgcccacacc cgtggggccg acgaagataa
     2041 agctggccgg acggcggcgc gctgagatgt ctgcgcggct gcgtttgaca gcgtttgcca
     2101 ccagatgcac ggcctcgggc tggccgatga tgaccctgtt caggttttct tccagatgcg
     2161 caagctttgt atactcggtt tcctttactt ttgcggccgg aatccccgtc caaagctcaa
     2221 tgacttttgc cacatcctcc atcgtcacct ggatgccatc cacggcctcc cgctgcgcgg
     2281 gcagttctgc ctccaggcgg atgatctcgg atttgatctg cgccagcttc tcgtagtccg
     2341 gcccctcctt ggcctgttcc tgctcagcct cctgctcttt caggcccgca aggtgctttt
     2401 cattttccac ccattcggtg atttccgggt ggcgcaggct gcagcaggca caggcctcat
     2461 ccaacaggtc gatggcctta tccggcagga aacggtcggt gatatagcgc tcggacagtg
     2521 tgaccgtgct gcgcacgatg gcttcactca cctttacatg gtggtacgcc tcataatact
     2581 ggcggatgcc cgcgataaca gccgtagcat cgtcgatgga cggctcctcg atcttcaccg
     2641 gctggaaacg gcgctccagc gcctggtctt tctcaatgta tttgcggtat tcggcaaacg
     2701 tcgtcgcgcc gatgacctga atctcccccc gggagagcgc aggcttaagg atgttggctg
     2761 cgttcatcgc gccctcggaa tcccccgccc cggtgatgtt gtggatctca tcaataaata
     2821 aaataacgtt gcccgccgct ttcacttcgc caatgagccc tttcacacgg ctttcaaact
     2881 ggccgcggaa ctgggttccc gccaccaggc ttgtcaggtc cagcagataa atttccttgt
     2941 cacgcaggcg cgccggaacg ttcccctctg cgatatgcag cgctatgccc tcggcgatgg
     3001 ccgtcttgcc cacgcccgcc tcaccgatga ggcagggatt gttcttctgg cggcgggaaa
     3061 ggatctggac ggtgcggtaa atttccctgt cgcggcccac gatgcggtcc agctttccgt
     3121 cccgcgcctt gcgggtgagg ttctcgcagt aattgtccag aaacttgcgt ttcttgccgc
     3181 ggtcgttctt actctttact tccttctccg gcgcttcttc cgctttcctg cggctgccga
     3241 aaataccgaa ggggaacgtt gcgttgccgc cgggcgtaaa atcgccgtcg ccgccttcgt
     3301 ccgtatcgtc gtccggcgct tcatccccgt ccgcatcgtc cggcggcgcc atcatcccaa
     3361 gctgctgcat catggcggcg gggtccatat ctccgttttc catcatgccg gcgaagcgtt
     3421 cttccatggc ctccagatcc tgatcggaga tgccgaactg tttcataata tcgtccacgg
     3481 gcttgatgcc aagctcccgc gcacacgaaa ggcagtagcc ctcgcttttc gcctcgcccg
     3541 cctccagctt ctgtacaaaa ac
//
LOCUS       DAWBSB010000227         3534 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_4393_length_3534_cov_3.274217, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000227 DAWBSB010000000
VERSION     DAWBSB010000227.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3534)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3534
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4393_length_3534_cov_3.274217"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          771 a         1176 c          993 g          594 t
ORIGIN      
        1 cggccttttt ctgcgccact ggctgctggt tttcttttcc gtgttgtttg ctgccgcgca
       61 catcgccatt acctggcaaa acacccgcgc gccataggcg ggcgtcccct ccataacaaa
      121 gcaaagcggc cgggcgtgca cagtgcacgt ccggccgcct tctgcatcat gccgtatatc
      181 cgctgtatgc gtggagcgtg gagaaactga tggactggat gacgcggtcc agcaccttgt
      241 cgtacaccag cacattgacg cccggacggt tgcggctctg ttcttctccg tccacggttg
      301 tggaggaatt gtccgccgcg ctgcgcagcg tcacatcgtg cccgccccag gaaagcgtgt
      361 attccagcgg gccggcgccg gtcttctggt acacgacttt tccgccgtcc accacatata
      421 ggccctgcat gccgctttgt tccagcggca gcgtgtccag cccccattgc ctgaacactg
      481 cgcgcggagc ggagggatcc gcttccgtgg cgtcgccctg tgtcgtgatg accgctgcca
      541 agtccttatc ctgcatcagc aggcggaaat attcggccat gtccagcgtg aagcgcaacg
      601 ttatattctg tgcgtcccgg tgctccatcc ttgccgcctc gtcccacgcc gcgtaggccg
      661 cgtcgccgcg cttgtcgggc ataccgtaat gctccgccag ataagccgcc agatccgtgc
      721 tgattttttg cgcgcctgcg gtgttcacat gggcatggtc ggccaggtcc gttgcatagt
      781 caaagccgat atcccgccca ccgggcgtgt tgtaatccag cacgggcaca ccgttttccg
      841 cgcagatgcg cgccgcgcgg cggaacacgc cgtactcggc ctcgctcgtc tcatagggcg
      901 tcaccacgaa caccagcggc acatcctttt ttctgcaaag tgcgataagc ttgtaaagat
      961 attcctcagc ctggggatag atgggcgttt cctccggttc cccttccagt atgggcacgg
     1021 ggaattcctc caccgtctgt aaagggccgt agccttttcc cgtggtcgca tgatcctgaa
     1081 aaaaccaggt gaaatcttcc gccgtcaggc tcttccaccg ggtgtggtag cgcagcatcg
     1141 gcagatagcg gtaaaacggc ggcgagttct gctggcaccg gctcatggcg acggcaagcg
     1201 ccgcgcggtt gagggacggc gggatgcgca gcgaataatc gtccgaggtg ccgtccacgt
     1261 ccgcaggcgt catataggtg gtgccgtagc acagcccgaa cccttccagc accacgaccc
     1321 tgggcgtctg ccgcttgaac gcctccaccg cgtaatggta gctcacccac atcggctggc
     1381 tccaggagta caatacatac cccgtgaaac cctgctcgcg ccacatctgc gcgggcgcaa
     1441 acgccgcgtt ggcatggctg ctgcccagaa agatcacatc gatggtatcc tccggctcgg
     1501 cataaaagcc gtcgatgcac ccgcccttgg gggccagcac ctcctgcgta cacaagagca
     1561 gcgccagcag gatgcacccg aacgccagcg cgcggacaag cggcggcagg ccgcgcgtct
     1621 gctttaagac cggtcctttc cccaatgttt acgccccccc gctcagaact ggaaatagat
     1681 gaacggcttt tccacatagc cctcgccgta cataccgaat atcgcccccg ggagcccccc
     1741 cgccagcagc acggcccatt gcacagccag cggcttttgc agcagccgcg ccgaaaggct
     1801 gccccaacgc cgccgcagca cgtcgcacaa aacaacagcc gccacgcaca cagccgaaag
     1861 caacaggtcg ggcccgtcca ttccccaggc gagcaccgct tcccggcccg ggaacgcgcc
     1921 cgggcgcagc aggctttgca ccatgctcaa cgccgcgtcc atgccgccgg cgcggaagaa
     1981 aatcatcgaa aacgccacca gcgcaaacgt ccacgccgtg cgccaaagcc gcgcgggcaa
     2041 acagccatag ggtacccgca gcgccgtata cagccgtttg cggaggggca gcgtcaggcg
     2101 gccgcacaca aggtacagcc cgtgcaaaag cccccatgcc acaaatgtcc atgccgcgcc
     2161 gtgccacagc ccgctgcaca gaaaaacaac catcgtgttc acacaggtgc gcgccagccc
     2221 cctgcggctg cctcccagcg ggatatacag gtaatcccgg aaaaagctgg acaggctgat
     2281 gtgccagttg cgccagaact ccgccacgga ttgagagagg aacggactgt ggaaattttc
     2341 catcagttcc acgcccaaaa tgcgcgccgc gccccgggcg atgtcggtgt aggaagaaaa
     2401 atcgcagtaa agctcaaagc agaacagcac cgtggccgtc gccgtggccc agccgcccaa
     2461 agcgccgcat tgggcataga cggtgtccgc cagcagcgcc agcctgtccg ccacaaccat
     2521 tttctgaata taaccccaca gcatcagcac aaggccgctt ttggcccgct cgtaggaaaa
     2581 acgggcaggc agctgcagtt ggggcaggat attgccgctg cgttcaatgg gcccggccac
     2641 cagctgcgga aagaaggaga taaacagcag ataccgccaa aagcgctgct cggcggacac
     2701 gctgccccgg tatacgtcca cagtgtaacc gatcgcctga aacgtgtaga agctgatgcc
     2761 gacaggcagg agcacgccca gcagcggtac ggcggggaag ccccgcgcag ccagcagacc
     2821 gttcgcactc tgcgcaaaaa aattataata tttgaagaaa aacagaatcc ccagatttgc
     2881 cgccagcgaa acggccatgg ccgccctgcg cctgccccgg ttcccggcgg ccggcgcacc
     2941 caataccgcc agcgcacgcc cgcacaaata ggtgctgaac gcggaaaaca ggatgagcac
     3001 aatataggcc ggattccagc acatatagaa aaagcagctg cacgcaagca gccatggctg
     3061 ccgcgcgcgg cgcggcagca cataataaac aaaaaccgcc gcaacgaaaa acgcacaaaa
     3121 gcgcagcgaa acgaagttca aagccgcaat cctcccctgt tcaccattcc aaggcaccac
     3181 cgggcctttt acggcgccgc agccgcagac agcccgtccg gtttcgcagg cggccgccgc
     3241 tgtgcgggcg gccttccaag cgaatgatac catatcgcac gcgggcttgc caagcaggat
     3301 atacgaaaaa aatgcctttt tgacgttccc ggtacgtcct gccggaaaaa taatacgctc
     3361 aacacggttg acaccgccgc cgccagcgcc tataatacat acggacataa aaacaggggt
     3421 gccgaaaggc tgagatttgt acgcgtacgc gtgtgcatga accctttacc tgatccgggt
     3481 aatgccggcg taggaagttt tggcgcagaa atgcgcaatt gggaaacttt ggcg
//
LOCUS       DAWBSB010000228         3532 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_4396_length_3532_cov_2.192407, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000228 DAWBSB010000000
VERSION     DAWBSB010000228.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4396_length_3532_cov_2.192407"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1741..1750
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          734 a         1060 c          956 g          772 t
ORIGIN      
        1 ccgaaaggcc tgcgtgggca tcaaggaggt ctctcccgcc gatatcgtga aatactcctc
       61 catgaagttg gaccatcttt cgggcaacct gtacaacatc accgacatga tcgagaagcc
      121 tgcgcccgga caggaattca gcctgttctc catcctggga cgctgcctgc ttacaccgga
      181 gatttacggc atcctcgaga agacgcagcc cggcgcgggc ggcgagatcc agcttacgga
      241 cgccatggcc gtgctggcgc ccaccggcgg catgacgggc gttgatttca ccgggaaacg
      301 ctacgacatg ggcaacaagc tgggcgtgat ggaggcccag gtggagaccg cgctgaagca
      361 tccggagatc ggcccggcgt tccgcgctta cctgaaagaa ctttccaaaa cgctgtaggg
      421 cgctgaaggc acaaggacaa aagttcttat tgttccgcgc cgcaaaacgc atttcagcgg
      481 ttacggctcc ggaacggccg cagagccgaa aacagcccgc ggctgttctt tacaaaccaa
      541 gtcataaaaa atgccgtgcc tgtccacaat atgcttgacg ggcacggtgt ttttattgta
      601 taattagttg cttgccgtga acaggcaggt aatccttgcc ccttcggggg ccttacgtcc
      661 aaaaggaggt gcacagaaat ggcaaagtat gaaacaatgc tggttaccac cgctactctc
      721 gacgaggaag ctacggccgc gctggtcggc aagttcaaat ccctcatcga agcgaatggt
      781 acgatcgatt ctgtcgagga ttggggcaag cgtcgccttg catacccgat caacaaggag
      841 aacgaaggcg tttacactct gatcaacttc acgagtgaag ccgacttccc cgccgagctc
      901 gaccgtgtct acaagatcac cgacggcgtg ctgcgttcca tgatcgtggc gaaagaagaa
      961 gccgttcccg cgaaagctga aacggaggca aaataatgct gaatgttgta gctttgatgg
     1021 gccgccttgt agcggacccc gaactgcgcc acaccccgaa cggcgttgcg acatgcacgt
     1081 tccgtatcgc ggtggaccgc agctttgtgc gtgccggtga agagcgcaaa gcagacttta
     1141 tcgacatcgt ggtctggcgc cagactgcgg aatttgtttg caagtatttc cacaaaggca
     1201 acctcatcgc cgtggacggt tccatccaga cgcgcacgta tgaggacaaa accggcaaca
     1261 agcgctatgc gtttgaggtc gttgcgaaca acgtccactt taccggcgaa aagagcggca
     1321 gcaccggcgg ccagggcggc tacggcgcgc ccgcgcccat gcaggctccc gccgcgcgtc
     1381 ccgcgcccgc agagcccgtc agctatgcgg ccggcagcgc cgacgatttc gccgtgatcg
     1441 acgacaacga agatctaccg ttttaaggag gcggacgtgc ccggcgcgcc ccttccgcta
     1501 ctttgagaca aggaggaaaa caccatgcct ttcgacagag accgtcagga tagccgtggt
     1561 ggccgccccc gtggacgccg caagaaggtt tgcagcttct gcgtggaccg tatcgaccac
     1621 attgactata aagacattcc ccgcctgcgc aagtacattt ccgagcgcgc gaagatcatt
     1681 ccccgccgcg ttaccggtac ctgcgctttc caccagcgtg agctgaccgt cgccatcaag
     1741 nnnnnnnnnn tttgttttcc tatccataat ccgacatgct ttgttcttat tttttatgat
     1801 aagtcaaaat ccacacgtcg aatagttgct tttgccatag tctattttcc aatagtatat
     1861 tataatcctc actgttcacg gccggctgtt tttgttagga taaatttaat caaattatcg
     1921 gatggtgttt tatcatgaac ctttggaaag accgccgcgt ggattctttg catcgcgtgg
     1981 tgctgccccg ggaagcgttc agtttgctgg gctggacgtc ggacgaaatg ctggaggcgg
     2041 aagccttgcc cgcgcaggat gctctgctgc tgcgcgccca aaaccatccg cgcccgcagt
     2101 gctgtgcgtg cggcggcgca caggaccttg tttccctggg cggcaggcgc tggctgtgcg
     2161 gggcctgcct tgccgccgcg aacgccgcaa gcaaagcctg atcttctcaa cccgccgcag
     2221 caaaaagcgc cccgcctgta ttaggcgtgt tggcgcaaga aaacatcgtc cttgaagcgg
     2281 gaatttccgc gcatagcttc gtcaaatcgc cttgacaacc acaaagggtg cctgcggcaa
     2341 ttttccttgc tctgcatcga aaattcccgt ttcaagggtg ctgcgcagtt ttggcccggc
     2401 ggttttggat gtacagtgcg cgaaaaaagc ctggcaatcc tttgtggatt gccaggcttt
     2461 ttgagtacgc tgtgcgccaa aaccgtcagc ccaaaaccga tgttttcttg cgtcaacgcg
     2521 cctttggcgg gacgcttttt tattgtgtcg tatcgcttta ctgcatcgcc ttttccgcaa
     2581 aggtattgtt tacaatcacg tcgtgcggcg ctgttttctc cagctcgcct gcggctgtca
     2641 tcaccgtttg cagcaaatcg aagctctcct gcgtgagcac cgggtccgtg ctgtacgcgc
     2701 cgatgtcctt gtagcgctgg acagcgctgg tcagcagggc aatatcggtg tccgggaagc
     2761 tgtctttgac cacttccgcg atttcggcgg cggaatgctc cgccacccat ttctggccct
     2821 tgtacacggc attggtaaag cgctggatca tatccgcgtt tttctcaata aagctctttt
     2881 tggcaaaata cgcggtgtag ggcatctctc ccgcttcctc gcccacagcc gccacgatgt
     2941 agcccttgcc ctcggcttcc acggaggatg ccgtcggctc gaacatcgtc acatagtcgc
     3001 ctgtgccgcc caaaaacgca cccgtcatgt tgtcgaactg gatgctgttg tccagcagca
     3061 ggtcggacgc cgggtccatg ccgtttttgc ggatggcata ctcaaacgcc atatacggca
     3121 cgccgccctt gcggcccggc aggatgtgct tgcccgccag cttatcccag gtaaagctgt
     3181 tgtccttttc gcgccccacc aaaaagctgc cgtcgcattt cgttacctgc gcaaacacct
     3241 cggtgtagtc ctctttaccc tcgttgtata catagatgct ggcctccggc cccgcaaagc
     3301 cgatgtccac gttgccggaa agcaccgcgc tcatcacttt gtccgcaccg ccgccgttgg
     3361 aaagctccac gtccagcccc tcgtccgcga agaagccctt tgccagcgcc acatactgcg
     3421 gcgcccagaa aatggaatgc gtcacctcgc acaccgtcac cttttgcatt tcttttgtct
     3481 tgccgctgca gcccgccgga gcggtcagcg ccagacacac acacaggaga ag
//
LOCUS       DAWBSB010000229         3525 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_4405_length_3525_cov_3.266282, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000229 DAWBSB010000000
VERSION     DAWBSB010000229.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4405_length_3525_cov_3.266282"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          819 a          973 c         1026 g          707 t
ORIGIN      
        1 ggccaagctg cccagcgatg gcatgggcgc ggaaacgccg atgcccagga acgaaagaaa
       61 gctctcggta aagatggccg aagggatctg cagtgtggtg gtgacgatga gcgtgccgat
      121 gcagttcgtc agaatgtgct ttttgatgat gcggcccgtg ccggcgccaa gagcgcgggc
      181 ggcggtgacg tactcgctct ccttcagcgt gagcacctgg ctgcgcacga tgcgcgccat
      241 acccacccag tacaaaagag cgaatacaat caggatgctg atgagcggca cgccgatgat
      301 gttgatccag ccgaagcccg gcttttccgc cagagactgc agcgggtatt tcagcgtaga
      361 ggccagcagg atgatgatga gcaggtccgg cacggtgtag atgatgtcca cgatgcgcat
      421 catgaacagg tctaccttgc cgcccagaaa gccggcaaca gagccgtaga ccgagccgat
      481 gagcagcacc agcacgctgg ccagcaggcc aacggacagg gaaacacggg tgcccatcat
      541 aacgcggacg gcatagtcac gccccaggga gtccgtgccc agaatgtggg gaaacacctt
      601 ttcgcccgcg gccatggcgg cctgctcggt ctcggaatac tgcatggggc ccaggttctc
      661 gctgccgcgg atctgggtgg cgtaatcgta agggtaaaac gccggcacga taaacgccag
      721 gatcagcacg atgatcagca ccgcaaggct caccatggcg atggggtttt tccgcagacg
      781 gcgcagaccg tccttccaga agctgacgct ttcgcgcatg acggacaggc tttccttttc
      841 ggaatcgctg gcgggcgcaa agtcgtcgac gttgagctgc atgctcaaga tctgtttttt
      901 gttttccatt ccttcgctct cctcccgctt atttcagctt gatccgcgga tcgatgatct
      961 tgtacacgat gtccacgatc acgttcatcg tgatgatgag cgtggccagg aagatcgtgg
     1021 ttcccatgat gaccatataa tcgcggccgg tgatggagct gacgaagctg tcgcccaggc
     1081 ccgggatcgt aaagatcttt tcaacgatca ggctgcccgt gagcgtatag gccaaagccg
     1141 gccccagata agtgacgaca ggcagaatgg cattgcgcag cgcatgctta aacaggctct
     1201 tgccctggga aagacccttg gcgcgggcgg tgcgcatgta atcctgcccc agcacatcca
     1261 gcatggaaga gcgcatcagg cggcagatgt acgccgtggg gtagaagccc agagcgatga
     1321 ccggcataat atagtttttc cagcccgtaa ggccgtagga cggcagccac cccagcttca
     1381 ggctgaatat atacaataat atggtacata caacaaagct cggcaccgcg atgccgcagg
     1441 tggcaataac aataatgacg ttgtcgatcc acttgccgcg gttgagcgcc gccacactgc
     1501 ccagcaccac gcccacgacg acggcaatag ccagcgcgat gccgccgatg cgcgccgaaa
     1561 ccgggaaccg ggaaccgatg atctgggcga ccgtgcggcc gcgctgcttg atggacgggc
     1621 ccagctcgcc ctgcagcagg ttgagcatat agttcttgta ctgcacgatc accggtttat
     1681 ccaagccgta cttttcctcc atggccgcaa gcaccgccgg ggacggcgat ttttcggaca
     1741 ggaacggccc gcccggaacc aggaacatga ggaagaaggt gagcatcgac acgatgacca
     1801 gcgtcaccaa tgccaacgca acgcgctttg cgatatattt cgccattttg ccaactcctt
     1861 taaagaaata acgttccgcc cggcccgcgc gagagccgcc ggagcaaact gtattttgcg
     1921 cgcgcacacc gcaaaaaaca gctttttgcc gcgcccgcag gagaaaaaag aatataaaca
     1981 aaaaatggtg tctaaaaaca acgtttaaac accatcgttt aacattctct gaatcatcat
     2041 tttaacaagt tttctatggt ttgtcaattt tactgtatgg atttttgttg ccatgcggcg
     2101 acaaatgttg ccaacggata catttttttg aaaaaaagcg tttctctgtc ctcaatgtgc
     2161 gtacactcat aacacaaaaa agcccgcggg cggtttcccc aaagggaacg cccgcgggct
     2221 caccgcgtgc aaaaaagcaa ttacgcagcc gtggaagcgc tggcgctttc gctggtgctg
     2281 gcgctctcgg caacctcaga ggtgctggcg ctctcagaag cgctggcaga ctcggaagtg
     2341 ctggcagacg cagaggtgct ggccggctcg gaagcgctgg tgctggccgg ctcagacaca
     2401 gacgtgctgg acggagctgt ggaagccgga gcgctggaag aggaagagtc ggtgccgcag
     2461 gcaaccagag acagcgccat cagcgccgca acagcaagaa caagaacttt tttcatagtg
     2521 aaaacctcca ttttttcgat tctttataca gcctctctgc ccgcgccgca gagtgctgcg
     2581 caagggcatt tgtaaaggct gtgtctgatt atagcatttt ttgcgtaaag gacaagatgt
     2641 tttttgtgca tttcaacgaa ttgtaactct tcaaacacac ttttttcaca acattcagaa
     2701 aatcgctcaa aacatcttcc ggccgcgaaa gcacttttag gcagattgga tatacattgc
     2761 ggatccggcg ctaagccctg ggcatgccgc aaaacacaaa ctgcccccac gcggccgctg
     2821 aaagccgggc taaaatagca aaggaaaaaa tttcctgact atatcaagct taaatcctgt
     2881 tgaacacatc gttcaaaaag tattttttct gaaacctgct ttttgacccg gcattcgttg
     2941 acaagcgtgc cgttcatgcg ctatgatggt attaaattcg gcgtatagag ccagccgccc
     3001 tgcggctgtg gatggaggga agctgtggat gtccggtact gaaacgctct ggcaggagct
     3061 gatctgccgc ctggaaggcg tgcaggcggc ccaggtggta tttgccgaaa acggcatgcc
     3121 gtgcgagatc cacgtgctcg ctgggcccga aaaaagttcg aaatcgctgg tacgcgatat
     3181 ccagtctgct ttgacggcgc agttcggcgt acaggtggac caccgcatca tcagcgttgc
     3241 acagctctcc gaggggcttg ccccccgggg ggatttccgt cttgcccata cggggctgga
     3301 gatcaaaagc gcgggcgggc gcgtgtccgc ttcggtaacg ctggcgcgcg gcggcgatac
     3361 gtataccggc cacggcgaaa gcgcgaatac gccttttgcc cggcgacggt gcgtttccga
     3421 ggccgcgctt gcggcagtga accgcgcggc gggtgaaacc tgcttcgaat tggccagcgt
     3481 ggatgcggtg acgctggcgg gacagggtat cgtggtggcg caggt
//
LOCUS       DAWBSB010000230         3519 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_4414_length_3519_cov_3.208430, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000230 DAWBSB010000000
VERSION     DAWBSB010000230.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3519)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3519
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4414_length_3519_cov_3.208430"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          670 a         1055 c         1089 g          705 t
ORIGIN      
        1 gccttccatg catttttctg cgcttgccgc agggcggcgt ttgtgatatc attcatttag
       61 acgagcgtcg aaagaaaagc agcggaagga gcggtgcgcg cgtgttcgac aaaacgttca
      121 aagccctgtc cgaccccacc cgccgccaga tcctggagct gctgcgcaga gggccgaaaa
      181 gcgccggtga gatatccgaa cagttcggca ccagcggagc caccatctcc caccatcttt
      241 ccgttctgaa agacgcgggc ctggtgagtg acgaaaagcg cggcaagtat attttttacg
      301 agctggacac cagtgtttta caggacatgc ttgcatgggt ggccggtttt gcaaagggag
      361 acggcgatga aacaaagcaa taaaacagaa tgggccatct ggggcgtgtg cgccctgtcg
      421 tttgccgtga cgctggcgct gctgcctctg ctgcccgcac gcatccccgt ccattggaac
      481 gtggacgggc agatcgacgg ctgggcggca cgcccgggag cgtttttcgg cccggccatg
      541 gggctgggcc tgagcctttt gatgaaattt ctgccctaca tcgacccgcg ccgccgcagc
      601 tatgaaaagt tcggccgcgc gtacgatgcg ttccggctgg cgctggcgct gttcgtgctt
      661 atcctgcagg gcgttacgct gtacgctgcg ttttatccgg atggactgga tgtttcccgc
      721 atcattgccg cctgtgtggg cgcgctgctg tgcgtagcgg gcaattatat gcccaagttc
      781 cggcacaact atttctgcgg tatccgtacg ccgtggacgc tggccagcga aacgtgctgg
      841 cggcgcaccc accgctttgc ggggccgctg tggttctggg gcggtctggc gctggcggcg
      901 ggcagctttt tcctctccgg ccgctggctg gccgcggcca cggcggctct gggagccgtg
      961 ctcgtcgtgc ccgcttatct ttattcgtac tttatttata aaagcggcgg tgaagattga
     1021 ttttgccgcc gcgatgcgtt atactatcct tggttaaatt tgtgacaagg gcttgtcctc
     1081 tttcactgtg ggaccgatac gcgctgggcg ggccaatgcg cttggagcgc ccgcccaaaa
     1141 caggaacgat tttttggagg gtaatgaatt atgctggtaa acgctaccga aatgctgcag
     1201 aaagcccgcg acggccacta cgccgtaggc cagttcaaca tcaacaatct ggagtggacg
     1261 aaggccgcgc tgcaggctgc gcaggaaatg aacagcccca tcattctcgg cgtgtccgaa
     1321 ggcgcgggca aatatatgac aggcttcaaa accgtcgccg ccatggtcaa ggccatgatc
     1381 gacacgctgg gcatcaccgt gcccgtggcg ctgcatctgg accacggctc ctacgagggc
     1441 gccaaggcct gtgttgaggc cgggttctcc tccatcatgt tcgacggcag ccactacgcc
     1501 atcgacgaaa acgtgtccaa gaccaccgag ctggtggctc tggctcacga gaacggcctt
     1561 tccatcgagg ccgaggtcgg cagcatcggc ggtgaagagg acggtgtcgt gggcatgggc
     1621 gagatcgcgg acgccgccga gtgtgcgcgc atcgcttccc tgggcatcga cttcctggcc
     1681 gctggcattg gcaatattca cggcgtgtac ccgtccaact ggaaaggcct ggacttcgcc
     1741 gcgctggaga agatccacaa cgcgacggat cacattcccc ttgtgctgca cggcggcacc
     1801 ggcatccccg acgatatgat caaaaaggcc atcagcctgg gtgtttccaa gatcaatgtg
     1861 aacaccgaat gccagctcgc tttcgccaag gccacccgcg aatacatcga ggccggaaag
     1921 gaccagcagg gcaagggctt tgatccgcgc aagctgcttg cgccgggcgc cgaggccatc
     1981 aaaaacaccg tcaaggaaaa aatggagctg ttcggctctg tgggcaaggc gtaagcgccg
     2041 cccacaaggc gttcagcgcc ttttaaagca acggcaaagc ccccggattt cccgaaatgg
     2101 gaaagcccgg gggctttttc tgcacaccgg cgggtctgcg tgcggcaccc cgccaaagcg
     2161 caaaaaagcg gcggccccat acggaaccgc cgcctgtgcc ggacatccgg cttatttcgt
     2221 ctgtacgccg ccgatgaaga ttttttccgg gtgcagcgtc tcgtccagca ccagtatgtc
     2281 agcgcgcttg ccggcggtga tgctgcccag ctcatcaaac aggcccacag cctgcgccgg
     2341 gttgatggta gccgcgcgca gcgccgcgtc caggggcacg ccgaagctga cggcgcggcg
     2401 gaagcattcg gccaggcagg tagcgctgcc cgcgatggtg ccgtcctcca gcgtggcctt
     2461 gccgtccgtc atatacacgg tctggccgcc cagcgaatac acgccgttgg gcatgcccgc
     2521 ggcgcgcatg gaatcgctga tgaggcacac gcgctcggcg ccgaaccagc ggaacacact
     2581 gcgcaccaca gccggatgca ggtggatgcc gtcggagatc atctccacat gggccgcatc
     2641 gtcgctggcc gcgcccacga cgcccgggtc gcggtggttg aacggcggca tggcgttgaa
     2701 caggtgcgtc acatgggatg cgcccgcggc aaaggccgcc ttggcctggt cgtaattggc
     2761 cgtggtgtgc gcaatggaca ccacgcattt tttgctggcc cgctcgatga aaggcaccgc
     2821 acccggcagc tccggcgcaa cgtcgatgag cttgatggcg ccgccggaca gctcatacag
     2881 gcggtcaaac atctcgatct ccgggtccac aatgtatttt tccgcctgag cgcccttttt
     2941 cgccttgttg aagaagggcc cctccatatt gatgccgcgc agcacggcgc cgccggtctc
     3001 tttgttgatg tagggcttcg caatgttgaa gatattggtg aggatgggct cgtcgaaagc
     3061 catcgtggtg ccgcagaagg ccgttacgcc ctcgccgccc agataggcgg acattgtttc
     3121 gatgtgctcc gcgcccgcgt cgcaaaaatc agagcctttg gagccgtgga tgtggatatc
     3181 cacaaaaccc ggggtgacgt atttgccgga aacgtccact gcgtcggggc cgtccagcgt
     3241 gccgggggca gccacctgag cgatctttcc gtccgcgatc tccacgtccg ccagcacaaa
     3301 gcgcgcgtct tcggtgaaaa caaggccgtt tttcaggatc atattgatac actcctttga
     3361 ttgattgtat ggtcttgcgc gccgggtggg gcgcgcggat ttccctgctt tcattataac
     3421 gtttttttcg gcgcgcaaaa gttttttttg caaaaaaagc gctttgtgcc aagattgcat
     3481 atgatacggc aaaacgcgca aaggccgccc ggtgccggg
//
LOCUS       DAWBSB010000231         3517 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_4420_length_3517_cov_2.062103, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000231 DAWBSB010000000
VERSION     DAWBSB010000231.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 3517)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3517)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3517
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4420_length_3517_cov_2.062103"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    915..924
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1172..1181
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2005..2014
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          793 a          883 c         1120 g          691 t
ORIGIN      
        1 cgggtgcggc gcagggtatg tgtaccatgg acgcaagcct gcggcgcctg gtgacggaaa
       61 agcgcgtcag taaagagaca gccctcacac actgcatgca ttatgaggcg atggaaaaac
      121 gcctttcagc aatgagctga atccaaattg attcggaagg gggagaggaa agtgcctgag
      181 caaagtaaaa tggaaatatc cctgttcagc ggtatgacga tcctgtggaa cggtacgccg
      241 atcctggagc acagcgcgcg gctcaacaag cctttggagc ttttggcgct gcttttgctg
      301 cgcggcgata aaaagctgac gaatgagcag ttgatggacg gcctgtggga gagcgatgaa
      361 atcgaaaacc cggccggcgc gttgaaaaac gcggcgtatt cgcttcgcaa gtttttgcag
      421 aaagcggata aggaaaaacg ctttatcatc acagagagcg ggcgttacat ttggaacccg
      481 gaaatctccg tcaccaccga tgtgtgggaa ttcgagcagg aggcgcgtct tgcggatcaa
      541 ccgggcacgc cggcggagga gcgtatcccg catgcgcggc gagcgctgaa gctgtatacc
      601 ggcgatcttc tgcccagcct gagcatgcag cagtgggtta tccaatacag cagttatctg
      661 cggcagacct atctgcgtac cgttaaaaat ctggctgcca cgctctgcga acgcggaggc
      721 agggaggatc tggaggagac gctggatatc tgcaaccgtg cggcgctgct ggagccgctg
      781 cacgaggaac tttaccgcta catcttcaat accatgcgca ggttggatat gaagcaggct
      841 gtgctgagct attatccggt gatctccaac ctgttttacg acgagctggg cgaacgtctt
      901 tcgcccgaac tgcgnnnnnn nnnnacatat atctctgggc ttcccaagga gcgaaccaga
      961 tgaaggaaaa tctgcgccag atccagcagg atttgggcga gattacgcgg gacgcccggc
     1021 ccatccacgg cgcatactac tgcgagtatg agatgttcaa atccgtgtac cagatggtcg
     1081 cacgaagcgc ggcacgttcc aatagtcatg tgctgctcat ccttgtgacg tttgaaggaa
     1141 aaaacggcca gaccgtggca aaacaggaga tnnnnnnnnn natcgtggac gccatggcgc
     1201 gcggcaagga agtcattcgg gagtctctgc gcaagggcga tgtattcagc cgttacagcc
     1261 gcaaccagta catccttatg ttgacggtca cttcccctgc cgacagcgtt gtggtggaga
     1321 accgcatcaa ggatgctttt aaggaagtgc gtctgagcgc gaaaatcgag ctgcgtatga
     1381 aagcgcagga gctggacccg atcgtttagt gtatgcaacc gcttcttccg gaaaacggcg
     1441 cttgcgtttt atgtgcgacg ggtgtagaat caaaacagcc ccaggaaaat aaaggacgga
     1501 ggaacgatca cgatgcgtac actcaacagt gtttctgaat tccagacaga ggcggccaac
     1561 gccgttttta caaagcagca ggcgatttcc gctacgttgc agctcctcac aaaagaatgg
     1621 aacgatcccg gcaatacgcc tgaggaaaaa tcggttctgg aaaatgcgat ccagcgcgcc
     1681 gaattccgct acatcgatgc gacgaagtcg gagacggacc gtatgctgga cgctattggc
     1741 gtggcgcgct ttactacgca ggatatcgta aacgccattc aggcgatcgt attcgatgcg
     1801 gagtgagccc caacctgaga aacattatcc ctgctgaaca gagccccgcc ggaaggcggg
     1861 gctttttgca tgcccggaaa cgaggaaaat ctcgccgcat actggtttgg cgtttagccg
     1921 gggcatatag cgcgcacact atttttgtgt ggaaaaggag gggatatgtt ggaaaaaagg
     1981 ctcgttgcaa tggcgggcgc gtgtnnnnnn nnnncagcag agcagtacca gcgtcgtgct
     2041 gagtgaaggg cgcggcaata tttacgactg cggttttttg ccgcttacag ggacggtctc
     2101 ggagcgctat gcgttgattg agccggggag gacgagctat catactttgt ttgaggccat
     2161 cccggcggaa ctgcgcacgc agttttatgc aagcatccag cggggctctc cgttcctgat
     2221 gcctgtcaca ggggcggccg cagcgcgtgc gcagtatacg tttgaaaagc ctgtgcggta
     2281 ccagcctatg cccatcgccc agcacctcat tggatatctc ggagcgtccg gccacggtgt
     2341 ttcaggtgtg gaatatgctt ttgatgatct tttgacagga ggctccactt tgacggaggt
     2401 gcgctgcgca atgaatgcgc gcgggggatt catagaatca gatgcgccgt acctggtgga
     2461 atctcccggc acgggggcgg gtgtgatgct cacgctggat tcaagcatcc agcgcgcctg
     2521 cgaagccgta ggcattgaaa tggtggataa gggatgcatc gttgtactgg acgcgcccac
     2581 cgggcgcgtg cgcgccagcg tgagcctgcc gctgtacgac ccggaaaatg tggccgcaag
     2641 catcgcgcgc caggatactt cattggtcaa ccgcgccctc agtgcatata atgtgggttc
     2701 ggtgttcaag ccgctgctgg ccgcggcggc gctggagcag ggaatcagcg cgcaggagac
     2761 gtatgaatgc acgggatcca tcgaggtggg cggccatgtt taccgctgcg cctatgggcg
     2821 gggccacggg acggtagaca tgaaaaccgc gctggagcaa agctgtaact gctactttgt
     2881 gcagcttggg ctgcgcctgg gcgcgcagac ggtgtacgac attacaaaaa gtgcgggatt
     2941 tgggcaaagc cagcaggttg cgggcggtct gcgggcagcc tccgggaacc tgccggacgc
     3001 ggcgcagctg gaagatctgg gccagctcgc atctatcagc ttcggccagg gagcgctgac
     3061 cgccacgccc gtacaggttg ccgcgatgat gaacctgttt gcaaacggag ggcggtatgt
     3121 ggaacctgtg tttgtggagg gaattgtgga tgaatacacg cagtcggtaa cggaaagcct
     3181 gtaccgcccc gtccagcggc aggtgatgga caaagatgtg gcggagggga ttcggcagat
     3241 gcttgtgggc gttgttgagg aagggctggg gcgaaaagcc gcgccccggg ccggcggtgc
     3301 ggggggcaag acggggacag cgcagacggg ccgctatcag gaggacggag aagaaattat
     3361 ggacgcgtgg ttcgtgggtt tttaccctgc ggaagaacct cgatatacca tcgccgttct
     3421 gctggacagc ggcacacacg acagcgacga cgctgcgcag gtgttcgcca aagtaacgga
     3481 tgccctgagc ttttttcttg cgcaggagta agccgtc
//
LOCUS       DAWBSB010000232         3516 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_4423_length_3516_cov_3.580179, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000232 DAWBSB010000000
VERSION     DAWBSB010000232.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3516)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4423_length_3516_cov_3.580179"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          659 a         1132 c         1046 g          679 t
ORIGIN      
        1 accggctgga gcacgccgac ttgatgggtg cctgggaact gcccgacggg ttcgtgcttc
       61 aatgggaagg tcctcccctc tttgtccacc acagcgatgc acaagctgtg cggtatatcc
      121 tttcccgctc ctacggcgtg ccgtaccgcg tcctcaggcc ccggcgggag ccgttttggg
      181 cactgtgcgc ctgtacggcg ggcctggcaa tctccctctg gctgagcggc ggcggtctgc
      241 ggctcctgcg gcagagcttc gccccctctg caccgctgcc ggatgctccg cagtctgccg
      301 ccgtaccgga tttttccagc acaaacggcc cttgctttgc cgccgcggac ggaggcgttt
      361 ggttcacggc ggggcacggc tccgctgttt cgccggaaac gacccgtgct ttctttaccg
      421 gggcaggccg cattttggcc gctgaaaccg gctccaaggt gcaggctgta ctgtatgcgt
      481 ccggcccgct ggattgcagt gtcagcgtca cgccggacga aggcgtcaca tggttcggtc
      541 agtcccttac cgcgctggac cgtcctgtcg aatacgcaaa gctggagttt ctgacgccgg
      601 agctgggctt tgcggcggtg ggcacgcagc attccccggg cggcggcgag gaaaaatatg
      661 cttttttcac ccataacggc gggcaaagct ggacggcatt cggcgccctg ccgcacttcg
      721 gcacctccca catcctcagc ggtttttcca tgacgccgga cggcacggcg tttctcactc
      781 ttgccacagg tccagaggaa aactggcccc tgctctactg cagtgcagac agcggagcct
      841 cctggcagac ggcggagctg ccctgggacg actgtgcgct tgcatacctg aaccacctgt
      901 cccgcgcgga gcagcggccc gagggctttt atgttcttct gacgcagctg ccgtacagcg
      961 gcgggaacgc cgcctttttc gccccaacgg cgcaagggcc gtggagctat gccggagatg
     1021 ccatcccaga tgcatgatcc acgcctgcaa agggacgggc cccgtgccgg atgcatcaac
     1081 cgcatcagca cgaggccctt ttttcaggca atgctcccgc ctgttatttc agtgtttttt
     1141 ccagattggc catgatgtct gccgcaccct cgctcatcac aagtacgatt tctccgccgg
     1201 gcagcgtttt gatcagcgca cccttggcaa cctcgtactg gtcggccaga tagttttcaa
     1261 acgacttgcg ctgcagcgaa acatagcttt ccaccgcagc ctgcacggct tcggcctggc
     1321 cttcggcagg cttgaacaca ccgatggcgt aggcccgcac attcatcatc gacatgctga
     1381 aggcgtacgc gtccagcgtt gcgggatcga tgcccatggt gtccagcatc atctggatgc
     1441 tggacgccgc atcctcggcg gacatatcgt tgggattcac ggccagcatc ggctcaccgc
     1501 ccgcgtcgcc gcaggcgatc tcggaatact cattgtcctc ggccggacgc gcgtcacgga
     1561 tggcataggc gtagcgctgg ccctcgctca tcgtgtcccc ggccccgccg gacgaaacgg
     1621 acgcggaacc cgaaacggaa cccgagccgg aggcggcagg cgtagatacg gcagaggaac
     1681 tgccggaatt cgtgcccggc agctttggcc cgcaggcggc aagggcaagg cagagcgcgg
     1741 cggcaagcgc cagcgcggaa atacggtaca gtcggttcat gtttgtttct ccttacagtt
     1801 tgtaatactg gcaaaggcgg ccgcacacca ggcggccttt gctatgaagt ataccccgca
     1861 gccctgccaa aaattgcacg cggggcaaaa tttctctaaa aaaggcaaaa aaggggcgca
     1921 aaagcgcccc ataccggcca ggccgcgggt tctttcactt atacttcggc gtggggtgat
     1981 aaaattcaaa aatcaccgcg tcgttttccg cttctttcgc gtttatatcg tcttcggggc
     2041 gcacggtcca gcatacgcgg atggcgccca ttgcctccgc cagacgggcg gtccatggct
     2101 ttttgccggg gccgtaagcg ataaactgcg ggcgcgccag cacgttggtg aacaggcagc
     2161 ccaatgcgaa tgccagcggc ttcggctcat cccgaaacga ggcgggcgcg tcggaaagct
     2221 gtccccgcag cacatccttc gcatttttcc ggaaccagcg cacgatgcgc gggtcaaagc
     2281 tctcgatgca gtaggggccg ttgtacgcgc gcatcagcgc cagccccttt tcgcaaagct
     2341 cgtcgttgcg ccgccccgtc ttgagttcaa tgatcatggg cgttctgccg cccatcactt
     2401 caaacacctc cgtcagcagg gggatcgtct cctgtgtgcc gcacagacgc atcccgcgca
     2461 gctcctcgaa tgtaaattcg tccacacggc cgtgcacgcc gcacacacgg tccagcgtgt
     2521 cgtcgtggaa caccacgacc tgcccgtccc tggagagctg gatatccagc tccacgccgt
     2581 agccggcctc cgccgccgcg cggaacgcgg gcaggctgtt ctccggcacc gtcttatcct
     2641 tcgtatgcaa gccccggtgc gcgtggttta cgccgtacac cagctgcttt tgcgcctcgc
     2701 cgtagcggcc cggccgcaga aaaagccaca gcagaacgat gcacacggcc agaacccaca
     2761 aaaataccgc cattcaagat catcccattt cccggcgcat acgcgccgcg tgatccggcc
     2821 ctgcaggcag gggcctccgg ggaggcgcgc cggtcctatc tgccatttta ccgcgctccg
     2881 cccgcgccgt caatcggcag ggacgcatgc gtaaaacatt ttcacaaatt tgacatatcg
     2941 gcgctgtaat atgctactat aagaaaaacg aaaaaaccat ctgctgccga aaacgaggtg
     3001 tttgttatgg caaaaatttt gatcgtagac gacgagccgc gcatccgcga gctgatccgc
     3061 gagcatttgg agcacgaagg cttccagtgc gaagaggcca ccgacggctc cgccgcgctg
     3121 gcggcgctgg ccgcctccgg agcggacctt gtgatattgg acatcatgat gccgtttgtg
     3181 gacggtatga cctgcctgcg cgagatgcgc aagcgcaaaa tcgacacgcc cgtcatcatg
     3241 ctgacggcac gcggcgagga atacgacaag cttgcgggcc tggagggcgg cgcggacgat
     3301 tatgtggtaa agccgttcag cccgcgggag ctggtggccc gcgtaaaggt ggtcctggcg
     3361 cgcaccatgc ccaaaacgca ggagggcggc gaaagctttg tattcgggga ccttgtcata
     3421 gacactgcct cccacagcgt gaagatcgcc ggggaggaag ctccccttac gcccaaggaa
     3481 ttcgatcttc tggtgttcct tgtatccaat aagggc
//
LOCUS       DAWBSB010000233         3503 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_4440_length_3503_cov_3.479698, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000233 DAWBSB010000000
VERSION     DAWBSB010000233.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3503)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3503
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4440_length_3503_cov_3.479698"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1591..1600
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          683 a         1142 c          967 g          701 t
ORIGIN      
        1 gtattgaagc tgcccgccac ctcttccacg gcggcaacgg cacgctccac atcctcgtct
       61 ttcaacacca taaataccgt ccggttctcc tcacggtccg gctccatcat ggcccgcagc
      121 gaacccagaa acgaaccgtc gcagtatttg tccaacgtca tggccatgcc ccgactggag
      181 agcacggtcg ctccgcgcag gcctgcatgc tccagcttat tcagtgcagg ctccagcttt
      241 tctacccggt tcagcacaaa aatcagcatt ttcatattcc cggtccgacc ccttcccgat
      301 tctttatccc tgtttccatc tatcagtata gcactctgcc gcacaaaaaa aaagggctgc
      361 ggcgcgtaca ggcgcaaaaa cagcgcgccg caagtcaaat ttttgtatgc gacgcgcctg
      421 ttttttgatg tcgtgtccta tttgatataa atgtggcgcg gcaggccgtc cacataaata
      481 ggggtaagct ccgcctgaat gagcggccgc atatatgcca gaaattcctg tgtcacacct
      541 acgccgtccg gtgtgatcca ctcgcccgga accttttttt caaaattcgc gatggtatgg
      601 atatcgtgca gctcggtggt gcactggtac gggtcgttgg aaaggcggcg caggcccacc
      661 atctcgccgc tgtgcccctc gaaagcagcc ttggccgccg cgccgcccac ctggaacgcc
      721 tcggtgatgt ccacacggct ggccatgtgg ctgctgcacc gctgcagggt ggaaagctcg
      781 atggcgcgtg ccttgcagcc cagctcccgc acggctttgt tcgccagaaa gcgcgccgcg
      841 cccgtcagcg ttttgtgccc gaacgcatcc accgggccaa gctcttccgt cagctcgcat
      901 acataacggc cgtcgtgtac gcgcacgccc tcagacaccg cgatgaccac gcctgccttg
      961 gcgcgctgca ggacgtccag ccgctcgatg aaatggtcga tgtcaaaggc cacctccggc
     1021 aggcagatca tatccacgcc ttcgcagtcc tctgctttgg ccaatgccgc cgcacccgtc
     1081 agccagcccg cattacgccc catcacctca acaatcgtga cgttgcgcgt gccgtatacc
     1141 aggctgtcgc gggtgatctc cttcataata gcgccgatgt acttggccgc ggagccgtag
     1201 cccggcgtgt ggtcggtgtt catcaagtcg ttgtcgatgg tcttgggcac gcccatgaac
     1261 ttgatgtcgc tgcctgtcat gcaggcatag tccgaaagct tggagatggt gtccatgctg
     1321 tcattgccgc caatatagaa aaagtagccc acatccagct ttttcaaaat accaaacagc
     1381 ttttcataca aagccgggtc ctctgccggg acgggcagct tgtaccggca cgagcccagg
     1441 tatgaggcgg gcgtgcgctt gaggatctca atgtccagat tatcccgcag cgtgtccgaa
     1501 aggtccacca cctgttcttc cagcaggccc ttcacaccgt accgcatgcc gtaaacatgc
     1561 tgtgcaccgc ggcttttcgc cgcctggaac nnnnnnnnnn attgataacc gcggtggggc
     1621 cgccgctctg cccgacgatc gcatttctac ccattttttc cactcctttg cgttccgcgc
     1681 ccggccggca ttttccggca aagcgcgccc ttgtcgcgga cgcacggcac gcccgccctg
     1741 tttttttacg tgttccagta tagcatgttt ttctccctgc cgcgagtgcg cacgccaaaa
     1801 aatacacgct tgttacaaat ttgataacga tttgacatcg ttccgacgca atatctgcgg
     1861 cttgtacgcc gtatatagag tagcagaacc tgacacagaa aggaagggaa ctcgatgaaa
     1921 aagacgttgt tcagtattct tttttcactg gcgctcgccg cctctctggc gggatgcgga
     1981 ggcgcggcca gcagcacggc cgcggcccgt gcttcttatt cctcgatgga cagcgcgccc
     2041 caggcatatg ccgcggatgc aggcggaacc gccgccgaag cggccgggac ctcggacctg
     2101 tccgacgccg tgcagaacag cgcggatctg ctgccccagg acgggcggaa gatcatcctg
     2161 aacgccacgc tctccatcga agcgctggac ttcaacgcca cctgtacggc gctggcgcgt
     2221 gccgcccagt cctgcggcgg atatgtctcc tccaccagca tcgatacgcc cgcttacgag
     2281 ggtgcatacc gcaccgcata ttatcagttc cgcatcccgg cagagcaata cagcgtcttt
     2341 ctggatggcg ccggcagcgc cggcaacctg gtgagcaagc aggaatccac ccaggatgtg
     2401 accagcgctt atgtggatgt ggaggcccgc ctgaaaagcc tgaagctgca ggaggagcgc
     2461 ctgtacgcga tgatggagca ggccggtgat ctggaaacgc tgctcgccat ccagaaccag
     2521 ctcacagaag tgcagtacca gatcgagagc tataccgccc agcagcgcac ctatgacgat
     2581 ctgatctctt attctaccgt agatgtaacg gtagaggagg taaagcagat caccgaaaaa
     2641 accgagacgt tcggcgaccg cgtatccgac gccttccgcc gcagctggcg cgacttcggc
     2701 tacggtgcac aggactttgc cgtgggcttt gtcgctgcgc tgcccacgct gctggtgttg
     2761 gccgtactgg cctcagtggc cgtgacggcc gcacgcgcgg ccgtccgcct tcataaaaaa
     2821 aggctggccg gccgcccggc tcctgcaccc cgcacgcccg ccgactatgc gccgcagaac
     2881 ggcaatgagg ctgaaacagg caaagacgct ccgccgtcca caccgcccaa atacaaataa
     2941 acgtacaaaa gtacaccggg agggcagttc catgaaacgc acgacattcg ctgcagcttg
     3001 tgtgctttgc cctgctttgc tgctggctgc atgcgcgccg caaggcgcgg cttccgcctc
     3061 cgcattcggc gacaggcctc ccatggacga aagcatttcc gcctccagcg ccgacgctct
     3121 gcctgcggaa gacgggtcga tcctgctggc ggagggcgcc gtgacgctga cgctgtccgc
     3181 cgaaaacctt ccgccgcctg gggaagcttc ttccgatgcg tccggccttg cggaaagaga
     3241 cagctctatc tcctacacca tcaccaacac cagcgggctg gagctgggcg tcatactcat
     3301 tccccgcctg gaacgccgga tggatggcgg ctgggaggct gtcccctgcg atgccgtctt
     3361 ctgcggaaca gccgatccgc tcgaaacaga acgttccggc cgctttccgt tggagtggta
     3421 cccggcgctt actcccggca cataccgcct gggtctccag ctggaggatt atggccgggg
     3481 ctgcctgccg gaggacgctt ggg
//
LOCUS       DAWBSB010000234         3470 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_4492_length_3470_cov_2.038653, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000234 DAWBSB010000000
VERSION     DAWBSB010000234.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3470)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4492_length_3470_cov_2.038653"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    649..658
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1674..1683
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          729 a          896 c         1064 g          761 t
ORIGIN      
        1 attatattct ggcggttacg cagataccgt tccttcgcta tgcgtggaat accatcgtca
       61 tcaccgcact gtgcgttgtg ggaaccgtga tatcctccgc gatggtggcc tattccatgt
      121 cgaaggtgcg gtggaacggg aaaaaatatt tgttcccgat catcgtggcc acgatgatga
      181 tcccatatca ggtcactttg atcccgctgt atatgatatg gaacaagctg gggctgatcg
      241 gcagcatcgc gcccctggtg gtgcccgcgt ttttcggcgc cggatactac atttttctgc
      301 ttcgccagtt ctttaagagc atcccggatt ccctgctgga agccgcggag atcgacggcg
      361 ccggagaggt gcgcattttt gcacagatca tgctgccttt gtgcaaacct gcaatggcaa
      421 gtgtcgctat tttcactttc atcaacacat ggtccgattt tatgggtcct atgctgtatc
      481 tgaaccgtca ggaaagctac acgctttcca ttggcctgca ggcttttttg cagcagcact
      541 atgtgcagtg gggcattctg atggcggctt ctgctatttt tacgatcccg atgatcgtcc
      601 tgtttttctt tgcacaaaaa tactttatcg aaggcatcac ggtaacggnn nnnnnnnnct
      661 tctggcaggc atgacagagg acgtcgctct ggtggaaccg cccgtcggcc ggatgggctt
      721 caaaaaaaag ccggcggata tcgacgcgct ctgggcctgg cttttcgaga atgcggggca
      781 atgcagccac gccatccttt ccgtggatac gctggtttac ggcaatatca tcaattcccg
      841 aacgcatcac cgccccctgg aggcatgcat ggaaacgctc tcccggttca ccgaactgaa
      901 gcggaaaaac ccggagcttt ccattcatgc gttcaatctg gtggcgcggg tggcggcata
      961 tgacagcgcc gcggaggacc cggattactg ggcgtcatac ggaagaaaaa tatggcgtta
     1021 tgcatacctg acggacaagg ccgagcgggg agaggcggat gaggcggagc gcggggaatg
     1081 cgccgcgctc cggcgcgaga taccggacgg cgttctggac gattttctgg cgcgccgcgc
     1141 ggtggaccgg gcggtcaacc ttgcgtgtgt ggacctggtg cgggacggtg tattcgatgt
     1201 gcttaccgtc ccaaaggacg ataccgccga gtacgggtat gcggcgctgg accagatggc
     1261 cattgcaaag agagtgcgcg agcttcggct gccggaccgc gtcttcgtat atccgggggc
     1321 cgacgaggtg ggaagcgtgc tgtttgcccg tgttttcaac cagatcaaaa actaccgccc
     1381 gcgcatttac ataagatatt cctccgtgta cggcccgttt attgtgccgc gctatgagga
     1441 ccggccgctg caggagggaa taaaagcgca gatcacctct ctgggaggcg tagttttgga
     1501 tgcgcccggt gattccgact gcatgctggc tgtaaacagc ccgggaaagc atatgattga
     1561 atccgtcgag caatatacaa aggacctcac cttttccacg catctgaatt tgaatgagct
     1621 tttccgttat atgcgctatt atcatgagga atacggccgt ccggtgggcc tggnnnnnnn
     1681 nnncggaggt atcggtagcc aatggatgcg aaaacgactg catggaattg ggactgctga
     1741 ccggaaccta tgattacctg accgctgtgg gaggctggaa caccgcgcag aataccattg
     1801 gcgtcgtgct ggcgcagctt gtgatcgcga gctattacaa tggcttttgc ggcaggccgg
     1861 agcagctccg gcggtcggag gagttcatgg cgagccgtct tgccagcgac tggctgtatc
     1921 aatccaatgt gctgcacagg taccttgtgg agactgcggg cacggtagac ccttatgcgc
     1981 tagggcggca ttacggggat gcggcggaat atttcaggca tgggctgcaa gcgcttctgg
     2041 acgagaagtt tacggccggg atacgcggcc gccgtcccat tgtggacacg ctgcaattcc
     2101 agtgggacgg aatcttttac atccggctgc atgtccggct ggaagaagtg ctccgcgcag
     2161 aaggtactaa ggaaaagaca gatacaggag aatgacgcat gatcacgaaa gccgctttgc
     2221 ttgaggacct ggaccgtgcg ggtatagacc cgcacggcat gctgctgtgc catttttcaa
     2281 tgaaaaagat cggccccgtg gaaaacggcg ccgacacggt gctggacgct ttgatggaat
     2341 acatgaaaaa tggattgctg atcgttccct gtcacacctg gagcaatgtc aacgatgaaa
     2401 accccgtgtt tgacgtgcgg gaaacaaagc cctgcgtcgg gcttctgccg gatctgttcc
     2461 ggcggcggaa gggcgtggtg cggacgctgc accccacgca ttcgctgtgc gtttacgggc
     2521 cccgggcaca ggcgtttgca gaagggcagg agcggttcga tacgccctgt gcgcccggct
     2581 cctgttacgg cgagctgctg cggcaaaacg cgcaggtgat gatggtgggg gtcacgtttg
     2641 cctgcaacac cagcgtacac tgcatcgagg agattgcaaa cgtgcccggc cgccttacag
     2701 cagagcatca tccgctgttt gtgcgcgatc aaagcgggcg gctgctttcc gtgccgtccc
     2761 gccgccatca aaacgccaat tcggaccatt acgtcaagct tgaaccggtg atgtttcaca
     2821 ggaatatact gcggcgcgtc ccgttcggca atgccgaatg ccttttgttc cgcggcaaag
     2881 acctgctggc cgttacgctg gagctgctga aaaaagaccc ggatcttttc ggcagcggcg
     2941 cgcccgtccc gccggagtgg tattgaatct gccttttacc gtgcggcaaa agggcgctct
     3001 gcggagcgga caaattgata ggaaagaagg aactgtcgtg aaaatattga actttggctc
     3061 tctcaacctg gataaggtgt ataaagtcgc gcactttgtg cgcgagggag agaccatcag
     3121 cgcacaggaa cagaaccagt ttttaggagg aaaggggctg aaccagtctg tcgcgctggc
     3181 aagggcgggt atctttgtgg agcatgccgg agcagtgggg cgggagacga cggaatttca
     3241 cgcgctgctc aaggctgagg gcgccggcac gacgtatctg cgcgttctgg aaacggtgag
     3301 cgggcatgcc gtgatccagt cctgtgcggg acagaattgt atcatcgtgt acggtggggc
     3361 aaaccggatg acccggccgc aggatgtgga cgctttttta aaggactacg gccccggtga
     3421 tctgctgctg cttcagaatg aaacatcctg tgtccgttat gcaatggaac
//
LOCUS       DAWBSB010000235         3425 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_4569_length_3425_cov_2.638279, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000235 DAWBSB010000000
VERSION     DAWBSB010000235.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3425)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4569_length_3425_cov_2.638279"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1397..1406
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2714..2723
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          794 a          876 c          979 g          756 t
ORIGIN      
        1 ctgctgcggg cggtgggcga caccttttac cccctgctgt tcatgatcgt ctccggcgcg
       61 ctgaacgtcg cgctgaatct gctgttagtg gtggttatcc ctttggatgt ggccggcgtg
      121 gccattgcca cctctgtctc tcagctgctc tccgcagtgc tggtgacggt ccgcctgtgt
      181 accttccggg gaccgtgccg tctggagctg cggaagatcc ggctgtatcc ggaccggacg
      241 ggccagatgc tgcggctggg cttctctgcc gggctgcagg gcgttctctt ctccgcctca
      301 aacgtgatga tccagtctgc catcaactcc ttcggctccg tggtcatggc gggcagctcc
      361 gccgcctccg gcattgagaa cttcatccac accgcgctga ataccttcta tcagtccgcc
      421 atcaccttca ccggccagag catcggggcc tccgatcccc gccgggccgt gcgggtcttt
      481 cggatcaatc tggccctgac aacagggctg ggcattgtgc tgtgcacact ggcccagatc
      541 tttcaggcgc ccctgctggg actgtacgtc cagtccaccg atccggccta cgacgcggtc
      601 atcgccgccg gtgtggtccg ggttctggca ctcagccggt tccagtgggt cggcggcctg
      661 atggaaacgg cctgcggcac cctccgggga ctgggccact ccatgaatcc caccgtcacc
      721 accctcatcg gcgcgtgtct gctgcgggtg gtctggcggt acaccgtgtt tgaagcggtg
      781 ggcaccgtgg agagcatgta ctggtcctat ccggtcagct ggctcctgac tttcctgatc
      841 cacttgatct atctggagcg tgaccgccgc aggctgatgg tggagaacac ggccgcccga
      901 taagcagcgg ctcccattcc ggatttttat atttctcata agaggaattg cgttttggca
      961 aaactgcttt ttgtgtgtca cggcattatt tgcagaaatc tctaaaatcc cctgtaatat
     1021 caaggttttt caggcttgaa ggtagtgcta ctacaccact gctacaccag tgagtctcga
     1081 tgtgacaaaa attttgagat cgaggtcaag tttatagata atatcttacc ggacttcatt
     1141 ttcgatggag tccggatttc tatatccaga agcagcattt cctgtgtcgg caaaaagtac
     1201 aacaatttcg ttttgaatgc tattgatatt tttgccgatg gcatggtata ctatgtaata
     1261 gattaacgtg gttttttgat ggattttatg gtggaggatc atggatgcga tatctttcag
     1321 ttgctgaaat agcgaaaaaa tgggatgtgt ccgagcgcag tgttaaaaat tactgcgccc
     1381 aggggcgtgt gaacggnnnn nnnnnnacac acgatcaaac ccaatatatc tttgaaacca
     1441 acaccatcgg cgtagaaaaa gaagtgctga atgtggatga cgtgatcgaa acagcaaatc
     1501 acttccgctg cattgacatg attattgatc atgcaaaagc agctctgacg gagaagttca
     1561 tcaaagaact tcacttggtt ttgaaaaacg gcaccagcga ttccagaaca gattgatttg
     1621 ctgttggcga gtataagaaa ctcccgaatg aagtcggcgg cagagataca gcccttccgg
     1681 aagaagttgc cgataaaatg aaggccttgc tgacggaata caacgccaag gaaaaaaaga
     1741 cttttgagga tattctggac ttccatgtga aatttgagca gattcatccg ttccaggacg
     1801 gcaacggtcg tgtgggcaga ttgatcatgt ttaaggagtg tctgaaatac aatatcgttc
     1861 cgttcatcat tgaggacgat ctgaagatgt tctattaccg tggattgaag gaatggaaca
     1921 atgaaaaagg ctatctgaca gatacctgcc tgaccgtaca ggataagtat aaagcgtatt
     1981 tggattattt taggattgca tattaagcaa gaagcgaggt gcagctatgt cagcagaaaa
     2041 gaactggcaa tttgaacttg aagaatcata aaaaataaaa tcccatgcaa taaatcgtcc
     2101 tttccgtgca ttacacggat gacaggagat ggaaacctat gctcagtatg ctgatgacaa
     2161 ccgaatatgc tccggcagag gatcggcacg agctgcagca gttgctgatc cacattgccg
     2221 gcggtgagcg tgaagcgctg gcggagctgt atcagcgcac gagatcggcg gtgtacggct
     2281 tagcgctgtc gtacctcaaa aacgcgcagg acgcgcagga tctcacacag gacgtctatg
     2341 tgcaggtgtg ggattgcgcg gcgcagtacc ggcccaccgg ctcgcccatg ggctggctgc
     2401 tggccgtatg ccgaaacctg tgcctgacgc gcctgcggcg ggaagaacgc catgccgccc
     2461 tcagcgagga ggaatgggac gccattcccg cgcgggagtg cggactggac gccgacgagc
     2521 gtgcgttgct gcaaggcgca ttggcaagcc tcgctgacga ggagcggcgc atcgtgctgc
     2581 tccacgctgt cacgggcctg aagcaccggg agagcgcggc gcttcttgag ctgccgcttc
     2641 ccaccgtcct gtcgaaatat cacagagcat tgaaaaagat gcgcagcttt ctggaaggag
     2701 atgacgcccg atgnnnnnnn nnnaaaatgg agcagagact ggctgcggcg cttgaaaaga
     2761 ccgcgccgga tgatgtgaac ggcgttctct cccgctgtga agagcggaaa ggaacggtaa
     2821 ttcccatgac aacaaagaaa acggcaaata gaaaatggac atcgctcatt gccgcctgcc
     2881 ttgctgtaat gctgctgggc ggcggaggcg tgttctacca gcgagcgaac gcggtggcgt
     2941 ccgtggtgtc gctggatgtg aacccgagca ttgagctgaa ggtgaaccgg agcgaaaagg
     3001 ttctcgtctg tacgccgctc aacgaggatg caaaagccat ccttgcggat atgagcaatg
     3061 gagctgacct caagggggca aagctcgatg tggcggtaaa tgccatcgtg ggaagtctcg
     3121 tgcgcaacgg ttatctcgac agcatttcct ccgccatcat gatctcagtg gaggacaagg
     3181 ataccgcccg tgcggaaaag ctccagcgtg agctgaccag cactgtggac ggtgttttgc
     3241 agacgagcga agcaaaggcc gccgtcctga cgcagacgct cacgcaggac gcggggcggg
     3301 aacagcaggc ccgggaaaac aacatttcca ccggaaaagc ggcgctggtg aaccacgtgc
     3361 tcgcactcaa ctccgcactc aagtttgacg cgctggcaaa gctctccgtc gaggaactaa
     3421 aggat
//
LOCUS       DAWBSB010000236         3420 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_4580_length_3420_cov_2.357207, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000236 DAWBSB010000000
VERSION     DAWBSB010000236.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3420)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4580_length_3420_cov_2.357207"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          711 a         1024 c          938 g          747 t
ORIGIN      
        1 aaaggcctgc gcacgggcga catgatgagc gagggctgca cgcctgtggg ctgcaaagcc
       61 atgggtgacg ccatatgcgc agagatttga ggccatgccg cacgctccgc ggctggtgcc
      121 tcaagcgcgc acaataaaac gggacggcga aaaaaacacc gtttctgcgc cgccgctctt
      181 ttagggaaaa gtataaaggt ttgggaacaa agaatcccgg ccgctgcgtt accttgcgca
      241 gggccgggat ttttcgttcg gctcgggcgc gcgccgctgt gcggcgggcg cctttccctt
      301 ttgcccgccg cgccgggccg ggcgcaggca aagcctgccg tattcatagc agcgccggca
      361 catatactcc tgaagctcct cgtagctctc caccattgca agcaggtccg ggtcttcatc
      421 atccgtgccc aggcgccggg cgatgcgttc ccgcgcatcg cccgccctct gccagatgcg
      481 gcttgctttt gaaccgggac gaaactcatg ccgaacaatg tgggaaagcg gcacacggta
      541 ttcctccaga atatattccc cgtgcatcag attataaata attttaaaga aatccataaa
      601 cccaaacctt tctatcccca ttttaaatcc ctatattggt attgtcaata cctattttag
      661 ccatttcaga ttgtgcatat ttacaaatcc aacctgtaca cttaagttgt acaggcggtg
      721 aaaaaatggg gaacagctat gcagactacc gggatgaagt tggtctgaaa atcaaatatt
      781 atcgtttgaa aaagggatat actcaagatc agttggccaa acgaatcggt attgaaggaa
      841 atcatctgag caagatcgaa cgagccaaag ttggcgtctc tttagacatg ctgtttgaca
      901 ttgccaacgc attgggaatt gatgcgtata agcttctcat gtttgattaa atggtgctga
      961 aatgaaatac aaatatacag actaccgtct tggcttggcc tcaaacatca agcattatcg
     1021 gttaaaagcc ggctacaccc aggaacagct ggccgaaaaa gtcggcatcg gttcaaaaca
     1081 tttgagccgc attgaaaccg cgcgtgtcgg agtatctctg gatatgctgt tcaatatcgc
     1141 cgatgcgctc ggggttgatg cctacaaact gctgatgttc cactaaaccg ccgcgggcca
     1201 gcccgcggcg gtttttcctg catttggggg agatttgtct tttctgcgcg ttgggattgt
     1261 gctataatca gaaccgttct ttttgtaaaa atgccaaagg agttcttaca atgggtatca
     1321 tggcacaaat cggcatcgtg cttgcggtct gcctggtagg cgagggcatc gcggcgctgc
     1381 ttcccatccc cttccccgcc agcgtcatca gtatggtgct gctgttcctc ctgcttgcca
     1441 caaagctgct tcggccgcac catatcaagg aaaaatccaa ttttctcatc aacaacatgg
     1501 cctgcttttt cgtgccggcc ggcgtgggta tcctgcgcta tgcggatgtg ctgtgggcca
     1561 acctgctgcc gctgctggcc atctgcttca tcaccacgcc gctggtgtac ggcgttacgg
     1621 cctgggccgt gcagctcacc atgcgcgcca ttcagaaaaa ggaggcccgt catgattgag
     1681 gctgtcacca cctcgccgct gttcggcatc gtgctgtgca tcggcacgtt ccagatcggg
     1741 cgctgggtcc agaaaaaaac agggctgaca ctggcaaacc cgctggtcat cgctattctg
     1801 ctgtgcatcg gcgtgctgct tatattcgac attccttacg aggatttcgc cattggcggc
     1861 gatatcgtga acatgatgct cggccccgcc acggctgtgc tcgccttgaa catctacaac
     1921 cagtatgccg tgctcaagcg ccacttcgta cccgttttag tgggctgcac cgcgggcagc
     1981 ctgttcagcc tggcaagtgt gctgctgctg tgccgcctgt tccggctgga cgaagccgtc
     2041 accgccgcgc tgctgcccaa aagctgcacc acgcccatcg ccgtaggcat tgccgaaagc
     2101 cagggcggca tcgcggccat caccatggtg gccgtgctcg tcaccgggtt catcggcgcg
     2161 gtgtgcgccc ctttgttcgc caagctgatg cacgtgacgg agcctgtggc cgagggcctt
     2221 gccatcggcg catgcagtca tgcgctgggc accacaaagg cggcggagct gggcgaagtt
     2281 cagggggcga tgagcggcat cgccataggc gtatgcggtc tgatcaccgt agtcgcggcg
     2341 ctgtttctgt gagcacggca taccggagca ttcaaaaagc gcgtgtgggc ttccacacgc
     2401 gcttcatttt tataccgttt tattgcccgc agcccggacc gggctgcacg gcggctgcct
     2461 attcatacaa aacctgcccc gcaaacacac agaccacccg cttcagatcc gggtgctccg
     2521 ccatcaactg gtgcgctgcc gtcagcacgg cgcccgcgct gggcgaagcc ggaatggcgt
     2581 ctgtctccag cacctcacgc gccgtgacgt tggccgtatg gctgggcacg gccatcacgg
     2641 catccacgat gtacgggtta tagttttccg gcacaaagcc cgcgcccagg cccgggatgt
     2701 tgtgccgtcc gatgtacccg ccgccgatgg cctggctctc gtaaggctct acggcaacca
     2761 cctttacatc cgggtaccat gccttgatgt attcgcccac gcccgtaata gtgccgccgc
     2821 tgcctacgcc cgcaacaacc gcgtccagcg cgccgcccgt tgccttggcg atgttcgggc
     2881 ccgttacacg gcggtgaaat tcagcgttca agtcattgtc gaaatagttc aggaaatagc
     2941 cgcccgtctg ctccacggcc tcctgcgcgc gctgctccac gcccctgcga ccgtaaacat
     3001 agttggaaag cacgatctgc gcgccgaact tcgtcagcat ctgctggcgc tccagcggca
     3061 cggtggccgg catgcagagc atcagcgggt ggcggctgtg ggcgcaggcc acggccagcg
     3121 ccacggcaaa gctgccgctg gaacactcga tcaccgtctg gcccggctgc aaaaggcctt
     3181 tttccgtggc cagagccagc attccttcgg ccagcgcgtc ctttgcggag cccgtcgtcc
     3241 cgccgtaggc cagatatgca tacagggaat ccggcagatc gtacttcgca cagtacccgg
     3301 aaagcgccac cacggggcgg ctgtacagcg tttcataata acttttgtgg atattcaaaa
     3361 cgaaacagct cccttcccgg cgaaaccgca cacaatatca cgattattat actataaaat
//
LOCUS       DAWBSB010000237         3417 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_4586_length_3417_cov_1.823022, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000237 DAWBSB010000000
VERSION     DAWBSB010000237.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3417)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3417
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4586_length_3417_cov_1.823022"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1030..1039
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1578..1587
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1962..1971
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2634..2643
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2954..2963
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          718 a          875 c          985 g          789 t
ORIGIN      
        1 ttttacgctg ccgccggaat tgccgttgtt ctgtctgctg agcctgcatg caaaccggga
       61 gaagcgccgg cctctgctca aaacgctggc gctgctgtgc gtattttata tcggactgac
      121 gttctgctcc gaggcggttt tgggtatgaa agcgcagctg cagcagcaga ccatccatac
      181 gctttcacgt ctgggcagca tttctgtatt ccgccgtctg gatgctctgc acattgctgc
      241 gtggatgctg gcggagctgt gtaaggtagc agcgctcgcc tacggcgtgc agagcgccct
      301 tacaccgctg ctgccccata gccagcgcgg aggcaaaacc tgcggatatg ctgtgggatt
      361 gcttgccgtg ctgctggccg tatgtgcggg cgcgccgcct gagacgctgc gcgcggtact
      421 cacggcaggt acggccgtgc tgctggcatg tacggtggtg tacggctgtg taatggaggg
      481 cttctatgca aaaaaacgcg tataaaataa ttgcgttgac actgcttttg atgctgctgt
      541 gcggctgtaa gcaaagccgc agtctggggg aacgcgccat tgtgaagatg gtgtacctgg
      601 acgagtccgg cgggcaggtg caggctggac tggtggtgtt cacctgtgcg ccgaattctg
      661 atacggcctc ggtggaagga caggctaaaa tttatacggc gcagggaaag agcattgaag
      721 aagctctgta caaggcggaa cagcagcaaa acaaaaaacc gttttatgcc cagaatgaga
      781 ttttgctttt ggggcccggc gccgcgcgca atgtaacgcc gtacctttct tattttgcag
      841 acgaaaacgc cgcgcgtcca aatcttgctg cgtttttaac gccgctgacg gcggaagagc
      901 tgtctgaatg cgaggacgtg atcagcgatg tagtgagaga aggcgaacgg ctgatcggaa
      961 tgggagccga tgagcaggat cgtacgcaaa gcatttttga gatcaatctt tcgggaacag
     1021 gcggtctggn nnnnnnnnng gatatcttcc cgtgttttca ttttccaaag aagagaagga
     1081 gtttcgcggg gtccggcaaa tggtgctgtt tcgcagcggc gcaccctatg ctgttttgga
     1141 ggacgctgca atgcagatgt ttcttctgct gaatgggaag gcacggcagc ttacagtgaa
     1201 cacacagatc gagggacggg tcgtatcatt ccggacacag cagctgcagc ttacgcatac
     1261 agcttccact gcaggcggcg caccgcatct tgaagtgtgt ctgaccggaa agctggacga
     1321 tgtaacggta gacggagccc ccgttgcgga caaagaagaa gcggaaatga cacgggagat
     1381 caacgcttac ctgaaccggg aagccgagat gctcaaccaa gcgacatttg ccaggggaaa
     1441 cgatacgttc cattatgcat ggtggctgaa gctgtatgat accgcagcct gcgaagcgct
     1501 gcttcgtacg gggacactgt acgacattgc aacggtggac ttttcatcag agctgaagcc
     1561 cgtgtaaagg ccctgcgnnn nnnnnnnaaa aacaaaaaac cgccccggaa tactccgggg
     1621 cggccgcttt tcttttaggg gccgggaaag gcttaatccc gcaggatttt atctgccaca
     1681 tacaggccgt gggcggccgc ctggctgagc gagcgggtga agccggtgcc gtcgcccaca
     1741 gcatagatct tttcgcagcc atccagtgca aaatcggtgc agtcgggacg ggcggaatag
     1801 tatttgcatt ccacaccgta aagcagcgtg tcgtaattca tcgtacccgg cgcgatcttg
     1861 tccagcgcgg ccagcgtttc cacaatgttg tccaactgac gtttgggcag gcaaaggctc
     1921 agatctccgg cgactgcatt cagtgtagga cgcacggtgc tnnnnnnnnn ncggtgctct
     1981 gggtcagcct tttttcatct gtgcggcgtc cgaccatcag gtcgccgagg cgctgcacca
     2041 gtacgctgcc gccgctgatg aggtttgcaa gccctgcgac gctgcgggca tattcgatag
     2101 gttccttgaa cggctcggta aaccggatgg tagaaagcag cgcaaagttg gaattctggc
     2161 tctttttggc ttcatccaca taggcgtggc cattgaccgt gagaactccg gaggtgtttt
     2221 ccataaccac ctgcccgcgc tcgttgaagc agaacatgcg cgtgacgtct ccgtactgct
     2281 tggaacggta ccagattttt ggctcgtaga tcttatggga aaaatcatcc cataccatgc
     2341 tgggaagctc gacgcgaaca ccgatatcca cctggttgtt tttcagcgga ataccgtttt
     2401 gagaacacca tttggcaaaa aagcggctgc ccacacggcc gacggcaaaa atgatatgct
     2461 ccgccgtgac gctgtcctgt acgcctttgc cttcataatg cacagtatga gcggctttgt
     2521 ccacatcggt cacgtcggta tccgtcagaa tggttacatg ttccttcaga aaagccgcca
     2581 tttttcgcat ggtctcgaaa ttggcgtccg ttcccagatg cttgagctgg gccnnnnnnn
     2641 nnnatatgca ggtcatactc cagacatttt ttcttcagct cgttgttggg catgaagcgc
     2701 tctgcaggcg cgccgaagtg ctgcaaaata ccgtctgcct gctcgatgta atccaaaacc
     2761 tgttcgggag gcagaaaatc cggcagccag ccgccgtatt ctgtggaaat gacgtatttg
     2821 ccgtcggaaa aagcacctgc tccggcaagg ccctccataa tggcgcagga ggggcatttg
     2881 atacaggtag gggcttttcc ggtgacgatg gggcatgccc gcttttcgat gtcgtgtcct
     2941 ttctcgaaca gaannnnnnn nnngctcgta agcgctcatc agtccgccga tgccgcctcc
     3001 gatgatcgca acgtcataat gctgcatgat tatctgactc ctttgctttt tgcttttgct
     3061 tttatcttca tcttggcctt atattcggcc attgcgcgct catatacgtt gagcaccgca
     3121 cggatgaact ctttttcgcc gtagcgctgc gtaaacgctg ttaccttttg gcggcgcagt
     3181 gcgcgctgct caggcgtaag agcggcttcc tcgcgcagca gcgcagccat ttcctccgcc
     3241 gtgttaaaga cgttgccgtt ttcgccgggg gtgatctggt ttttgttgta gatgtccagc
     3301 cgctggatca cgtacagtcc actggccata gcctccagca tggaaatgga attcatttcg
     3361 gacaatgaag cggtagtgaa aaggtcgcat gcatggtaat agggcggcag gaactgg
//
LOCUS       DAWBSB010000238         3372 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_4645_length_3372_cov_2.822731, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000238 DAWBSB010000000
VERSION     DAWBSB010000238.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 3372)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3372)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3372
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4645_length_3372_cov_2.822731"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    318..327
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1336..1345
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2341..2350
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          661 a          974 c         1038 g          669 t
ORIGIN      
        1 ctatgccgag gtgctgggcg cgaagctgct gcccgtggca gtgaaaaaca ccgccgtgat
       61 tttggtggtg agtattttct tcaacgtgct gctggggtcc gttacagctt attgcctgca
      121 gcgctttgag ttccgcttcc gcaggctggt gatgggctgc ttttacctgg gcatgctggt
      181 gcccaccttt gtggtcgaga tcgcccgctt caaggtcatc cagagcatgg gactgtacaa
      241 cacgctgggc gcgcccatca tcatctatgt ggcgtccgac ctgatgcagc tgtacctgta
      301 catgcagttc gtcagcannn nnnnnnnggc tatttccgca ttttttacag catcattttt
      361 ccgcttctgg ccccggcaac ggccacggtc gtcattttga agatcgtcaa catcgtcaac
      421 gacatgtacg tgccgtacct gtatatgccg aaaacaaagc tcaaaacact gaccacattt
      481 ttgatgaatt acgcgggtgc gcagcagggc tcctggccca cgctggcagc cgccatcatc
      541 gtagtactca tccccaccgt agtgctgtac ctgattttcc acaagcagat cacggaaggg
      601 ctttcggcgg gcgcaacaaa ggaataaacg gaggtattga ccatgagcga atttatgtct
      661 tacgatccct gggcggagat caccacccgc aacggctatg ccggcgacga aatggtagcc
      721 atgctgcaaa aggccatccg caggggcaag gaagaggacg cgctgcacgc ggcctatgaa
      781 atgtacatca ccagccccca gtttgaggaa aagctgtggc gccgcctgct gtgcatcagc
      841 gtggaggata ttggcttcgg caatccggat gcccccaacc tggtgtacac gctgttcaaa
      901 atgcgccagg aattccccta caacgacggc gacaggccca tgttcttcgt gcatgccatc
      961 cgttacctgt gccgccagaa gaaagagcgc tccagcgacc atgtgaaaaa tctgctgaac
     1021 cacgagtttg aagtggggac aaagtttgaa gtgccggatt acgcgctgga catgcatacc
     1081 cgccgcggcc gcgagatggg ccgcgacgtg taccacttcc tgacggaggc cagccgcgtg
     1141 gagccctatt acgagaccga gggcgcggcg gagatctatg aaaaatacaa ggcgctgctg
     1201 gaaagtgagg accaaggcga aaaatgcccc aatgcgtttg aattcaactc ctggcagtat
     1261 tgaggaaatc cccggttttg gcattgccaa aaccggggat ttttgttctg cggctcagtc
     1321 gatgactgtg cgggtnnnnn nnnnnggtgc ggccgatgac cacgctggcc gccgtcagct
     1381 ttttccaggt gttgcagccg cagatgccgt ccgccgtcag gctcacggcg cgctggaacg
     1441 cacgcaccgc gttttccgtg ccgccgccga agatgccgtc cagcgggcgg ttggtgtagc
     1501 caagcgcatt gagggcatcc tgcaggatca gcacataggt gctgcggctg ccgcgccgca
     1561 cggtggggta gcccgccgtg gtgccgctgc acgcgggagt accgtagcgg cggtcgaaat
     1621 gcacccaggt cggcgtcatg gagatgggct ccacatagcc ccaggcgccg gttgccacag
     1681 ccgcgttgcg gatggccacg cgctgggaat acgaaacgtt ctgccccacg tcgaagctca
     1741 cgcccgcgta gtgctggctg gccgttccgt ggccgccttc ccagatgcgc ttgaacgcat
     1801 agccgatgta aatgccctta ccgtaacgcc gccgcgtaag gttccatgcc tccatggcgc
     1861 gcgtcgtcgt ccaaagcgta gggctgttgg atgagccgcg gaactcgcgc acgcgcatcg
     1921 tgttgccgta ggcgtagggc atcgggtcgt tttcagacag gttataggtg aacagacgat
     1981 tggtgtactc gtcgtatacg atgaccttgg ccatggccct attcctcctc catgatgtaa
     2041 taattgtaaa tggcgtccca ggtggcgcgg tccacaaggc ccgtcacagg caggccgaag
     2101 ccctcctgga attccttcac ggcattgagc gtaatctcgt cgaaaatacc gctttcgggc
     2161 acgaaccagg ccacgcagaa gcgggaggcg atgccgttca tgtagcgctg caggcggcgg
     2221 acgcttggcc cggcgctgcc gattgccagc acgaagccag ggtattcacc ctccggcaga
     2281 tcgttttcgc cttcgtcgga ggcaaggctc aggtaagtga tgacgagcgc cgcccaggtg
     2341 nnnnnnnnnn gcgaactccc gctggaagct ctccacggcg tccttggttt cggggccaaa
     2401 ttcgccgtca agcacggtga tgggggagat ggtgtcgtag aacgagccga tgtacgccag
     2461 catgaactga acgaactgca ccatactgcc gctcatgccc tcctgaaggt catagcccgg
     2521 gtatgcaaac acacggaagc ggttcaccgg gccgtccacg ttgcgcagcg tggagaactg
     2581 gttgtaaatg ctgttccagg tggcggggcc gacgacgccg tccaccgcca ggccgaacag
     2641 tcgctgatag gcccgcacgg cttccgccgt tgcggtgccg taaatgccgt caaaggcgat
     2701 gacgggaatt tccgtgtagt aggcgctcat caggtacagg taatactgca cttccttgac
     2761 cgcccgtccc gtagagccga ggcgcagagg cgcaccgggg taagtgcccg ggcgttcggt
     2821 gggcgccagc aggccgttga tgatgcctgt gtacacctcg tagatcttgt tccaggtcag
     2881 cttgcccacc agaccgtcca cggtaagccc gtagaattgc tggaaagcgc gcacagcacg
     2941 ctcggtggcg gagccgaaga tgccgtccgc cgtgatggcc gggatggcgc tgttggagtt
     3001 ggagatgatg cgcagataaa actgcatttg cttgacgtcg ttgccgcggc tgcctacagc
     3061 gagcgtggtg ccggggaact ggcccggagt atcgacgttg ggcggggcga tatctgtctg
     3121 gatagaactg tattcgttgt agatgctgtt ccaggtcgcc tgtcccacga tgccgtccac
     3181 cgtcaggccg aaatgctgct ggaaggcgcg cacggcccgg tccgtggcct ggccgaagat
     3241 gccgtccacc gtgacggtgg gaatggtgtc cacaaattcg gccacggcgt tgagccagaa
     3301 ctggatctgt tccacatcgt cgccgcggct gcccacacgc agcagggtgc cgggccattg
     3361 gccggctaca ag
//
LOCUS       DAWBSB010000239         3368 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_4648_length_3368_cov_2.078781, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000239 DAWBSB010000000
VERSION     DAWBSB010000239.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3368)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4648_length_3368_cov_2.078781"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          749 a          908 c          991 g          720 t
ORIGIN      
        1 gaaaattttc gcagtgcgca cacagcggca cgcatcggaa gctctggaaa ttcagcctcg
       61 gctttgtccc aaagattttc gtgcaatgcg ctgcacccgg ggaaaaaagc gctgaggcgc
      121 accgcctgtt gcggtgaaca ggcccagccg agttccaggg cagtctgcag agaatgcgtg
      181 tccccgggtg cgagtagaaa gcggaagaag cctatggctg cgcgtaccgc aggctcagac
      241 aaaaagctgt cgcgcccggc tacggtatag gggatgtttt ctttttgcag acaatattcc
      301 agaatgtctg cctggcggtg ggtgcgatac aagattgcaa tatcggaaaa gctgcggggt
      361 tggatgcgtt catcacctgt gactccgccc gcgtccagca tatccatacc gccaaccata
      421 cggttgatct cttttgcaat aaagatcccc tccgcaaggg aactggccgc ctgtgccaaa
      481 cgcaccggcg ctccgctgcc gcggttgggc agcaaactgc gcgcgccgcc gggattgtgc
      541 ccgatcacag gcagcgcgca gccaagcacc tccggcgtgg agcgataatt ttgggtaagg
      601 cgaacgacac gcagcccggg gatatcttct gccaagcggt cgaagcatgc tgcatccgcg
      661 ccgcgaaagc cataaataga ctggtccggg tctccgatgg caaaaagact tttgcagcct
      721 tttgtccatg cccgcacaag acggtattgc agcgcgtcaa tgtcctgaaa ctcatccacc
      781 agaagataag taaaacgctg cgttatctct tccgggagct cacccgcttc aaaaagcgcc
      841 agcgtttcca ggagcaggtc atcaaaatcc agcacatcca gctcgcggca gcgggcgaca
      901 taagattcac agaacgcggc attcacattt tccggcaccg gcagtccgtt tttttgccgg
      961 gaaacagcct gcaccagctt tgcaggcggc agctttgcct cggcctgacg gagcacgtcg
     1021 gcggcaacgg cctgtgcatc ctcctgtgag agaagcgtca cggctttgcc tgtctcccgc
     1081 agcagtgaaa ggcagatgga atggaacgta ccgatggtca ggtcgccgat tgtgcggtct
     1141 ttgtccagct cagcggtgag ccgttcgcgc atttcacggg ctgctttatt ggtaaaggtg
     1201 acggccgtga gctgccgcgg ggacacctgc ttttcacaca gcagatgaac ggcgcgggaa
     1261 accagcgttt tcgtttttcc ggtgccgggg ccggcgataa cagccacagc gggttcgacg
     1321 gcgcagacgg ctttctgctg ctccggattc agcttgctgt gagcggtttg taccggcgcg
     1381 gaagactttg cgccggatga cttttggggt gcgtctgttt ttcggggcct gcgtgcagaa
     1441 gcttcgggat gcggcgcgtc ggaactgaaa aaactgatct gtccgttaag agattcgatt
     1501 tcatcggggg aaagaagttg gacggttccg tattgcccat cgaagcccgg cgtgcgggac
     1561 acctgcccgc accgcaggcg gcggatgcct tcttccacac aagggccggc cgcacggcgg
     1621 atgtcctcca gcggcgcttg gcgcagaata taaaactccg gcccaagctc ctccagaagc
     1681 gcttgatacc gcgcggcgac cttgagaccc gaggcggaaa gtccagtgga cgcagcaatc
     1741 acatccggca gcggcacaag gctttcaaac ggacgcgcgc cgggcaggga aaagccttca
     1801 ggtctgtcgg cgagctgctc cacccgatgc tgcacgccaa tggtgatttt tttgccgcag
     1861 acagggcatc gtcctccgca ggcttcggtt tcacccggct ccaggcacag accgcaggca
     1921 cggtggccgt cccaatggta cttgccctcc tcggggaaaa attccagcgt tcccgtcagc
     1981 gcggcgggac tgcgcccttg cagcgcatcg gataagcccg cgtaagacgg ttcaatgtcc
     2041 aacaggttgg cctcgcgccc aagtttggcg ggagaatggg catctgaatt cgagatcagc
     2101 gcatatccat ccagtgcgga aatgcgccag ttcatgggag gatcggaaga aaggccggtt
     2161 tcaagcgcgt ggatataggg agtcaagtcc tcaaagcatt cctcgatggc gtcaaaacct
     2221 gagaaagcgc cgaacagaga gaaatgcggc gtccagatat gtgcggggat aaacactgcc
     2281 tctgggcagg tgtccagagt gatttccagc aggtcacggc tgtccagacc caggattggc
     2341 cgtccgtccg agtggatgtt gccgatagcc tccagcttct gggaaagcct ttcggcagcc
     2401 tcgagccccg gcagcaggat cagattgtgc accttacgcg ttttgccgtt ctttttgtag
     2461 atggagctga tttctccggt cactacaaaa cggggtgcat cgtatttgcc cgcggtcttg
     2521 tccgccaggc gaaggctttc ccgtaatgtg tacaggccgt cctcagcagg ctgcagcttg
     2581 tccagtagtt cagcacgcca ggcaggatgc gtaaaatctc cagtgcccac cagcccgatg
     2641 cccttgcggc gtgcccaaag gtccagcatc tccggttcac agtcatggct ggtagcgcgg
     2701 gaatatttgg aatggatgtg aaggtctgca atcaacattg tatcacctgc ttgctttgaa
     2761 tttcctttat ataccatgcg gctttgcatg gttcaatgtt cttaaagctc tgtgcgcgag
     2821 cgcagaagcg tgtgcaaagc acacgcacag ccgcgtaagc ggcagttttg aggttattat
     2881 accataatgc tgcggcctgc tacaataaaa gcgcccgtac ggcgcggcgg aaaaaaacaa
     2941 tgtttttgca gatttctcag gttctgggcc ggccgatgtt acgggcgtaa ggggagaagg
     3001 atatttttgc gtgagcctgt tgacattttc gaaagaagat atataatatc aattgaacgg
     3061 atgctcatat gaaaggatgt gctttttgtg gaactgagcc aaaacgaacg ctgcgaatat
     3121 atgcatgtac acgaagacat tgtagccaaa gtggagaaag agatgccgga tgacgagatc
     3181 ctgttcgatc ttgcagaact gttcaaaata ttcggggact ccacgcgcat caaaattttg
     3241 tatttgttgt ttgaatcgga gatgtgcgtg tgtgatatcg ctcagatgct gcgtatgacg
     3301 cagtcggcca tttcccatca acttgcggta ttaaaaaaga gcaagctggt aaaatacagg
     3361 cgggaagg
//
LOCUS       DAWBSB010000240         3358 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_4660_length_3358_cov_3.688768, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000240 DAWBSB010000000
VERSION     DAWBSB010000240.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3358)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4660_length_3358_cov_3.688768"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2760..2769
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          607 a          911 c         1145 g          685 t
ORIGIN      
        1 cattcagggc gtggaggact tccacggcga tatggacttc aaggtgggcg gcacacgcga
       61 cggcattacg gccatccaga tggatatgaa ggtagacggg ctgacctacg agatcatcga
      121 ggaagccttt gagaaatgcc gcaaggcgcg cctgtatatc ctggacgaga tcatggcccc
      181 ggtcatcgcc gagccgcgcg cggagctttc caagtacgcg cccaagatgc tgtccatgaa
      241 gatcgatgtg gataagatcc gcgacgtcat cggcaagggc ggcaaggtca ttcaggagat
      301 ctgcgccaac tgcaacgcga agatcgacat cgaggaagac ggcagcgtat tcatctccgc
      361 catcgacatc gcggacgcgg agcgcgccat gcacaccatc aagaccattg tggaggaccc
      421 ggagatcggc gccatctata agggcaaggt cacccgcctg atgacgttcg gcgcgttcgt
      481 agagatcgcc ccgggcaaag agggcctggt gcatatctcc aagctggaca ccaagcgcgt
      541 ggagaaggtg gaagatgtcg tggctgtggg cgacgagctt ctggtaaagg tcaccgagat
      601 cgaccagcag ggacgcatca atctgtcccg caaggacgcg ctggcggact tggctgcaaa
      661 gcagaagtaa ggaaagtcca cgctgcccgg gacgcttcgg ccgcgtgcgc tgccgaagcg
      721 cacggtacgg cagccacgga accaggcggc aaagcagaat agaaacgcca aaaggcggcg
      781 cagcggtttt gctgcgccgc cttttggctt gtcgaaaaag ctgcgctttc ccgccaagct
      841 gcctgcggac agggcctttg gccccgttcg ccgcgcacgg cggttttcgg cggtcgcttc
      901 gctctacacg cctcggcact ggccctgcgg gacgtcgcgg ggttatttta aataaaaaag
      961 gggcatctgc gcgctaagag ccgcgctttc agcgcggttg tccgcaaggg aggcttcgca
     1021 ccgctccggc acgcgcctgc gggcgcagtc accacccctt tttcaggctg cccgcagccg
     1081 ggacgaccaa acaaattttt gcatttctga cggttatttg ttacaatggg ttcagtttta
     1141 tagctgcggc ggaaaagatc tgctctgccg cacagaaggg gagagggaac gtggcaattg
     1201 aaaaggtacg ggaatatttt gaaagtttcg gcgccgctgc gcgcatccgg gaatttgatg
     1261 tatccagcgc cacggtggaa ttggccgcgc aggcgctggg ctgcgcaccc gagcgcatcg
     1321 caaaaacgct gtcgttcctg gtggacggcg gttgtgcgct ggtggtggcg gcgggcgacg
     1381 ccaaggtgga caatccacgc ttcaaggcgc agtttcacac caaggcaaag atgctcaccc
     1441 ccggcgaagt ggaggcgatg gtgggccacg ctgtgggtgg cgtatgcccc tttgctgtca
     1501 acccgggcgt aaaaatatat ctggatgctt ctctgcggcg gtttgaaaca gtgttccccg
     1561 cctgcggcag cagcaacagt gccattgaac tgaccatctc ggagctggaa cagtattccc
     1621 gtttcgatgc atgggtggat gtctgcaagg gatggcgggc ggaaggggaa ggctgaaagg
     1681 cctttttcga agaaagagcg tgtgcccaca gggacgctgg aaagcaggag gaagccatgg
     1741 acgaagcggg tgaaaaactc aaacgggcgt ttacaggcgc gttcgcgctg ctgttcgccg
     1801 tcatcgtgct gtgtgcggtt ttctgcaacc agaacgtgtt cggcacgcaa agcccgcgga
     1861 gcctgctggc cggggggctg gcgctggctg tgggcgtgct gtgcgccgcg tggctgctgc
     1921 gcctgctgtc cgccccgggc aaaaggctgc gccggacgct gctggcggcg tttttcacgc
     1981 tgtgcttttt ggcgcaggcc catgtgggca gggcgctggt ggtgggtgct accggagact
     2041 gggatttcgg catcgtgctg cgcgccgcgc tggactttgc ggcagacggt gcgcagggcg
     2101 gtatttattt caacaatttc cccaacaacc agccgctcat gctgctgttg gcggggttgt
     2161 tccgtctgct gcgggcctgt ggtgtgacgg aggagtatga gctgctggtt tggggcatgc
     2221 gcttcggcgc ggtgctgctg cagcttgcac tgttctttct gtacctatgc gccgcgcgtc
     2281 tgctgggccg tcgggccggc gcgctggcgc tgctgctggg tgcagccaca gcgccgtttt
     2341 tgctgtatgc acccgtctat tatacggata ccgtcaccgc gcccattccc attgccatat
     2401 tgtactgctg gctgcgcctg cgcgcggccc gacgggaggg gcggccgggc cggggcccgc
     2461 tggcggctgt ttttgtgctg ggcgttttgg ggacgctgct taaagtgacc gtcgttatca
     2521 tggtcatcgc cgtggtgctg gattggatga tctcgttccc aaagctgcgg gagcgactgg
     2581 cagcgctggc tttgttggcc gccgtggtgg cgggcgtgaa tgcgggcagc cttgcggccg
     2641 cgcacagcgg tgcgttccgt aaggcgcagc cgggggatga gatacccaaa acgcattgga
     2701 taatgatggg cctcgcggga aacggcgggt acaacaacga ggattatgtg ttgacacttn
     2761 nnnnnnnnnc ggacggtgag acgcgcaagg cgcttatccg ggacgagatc gccgcgcgcc
     2821 tgaaggagat ggggcccgca ggtctggtgc ggcacgcact ggataagatc ggttatacgt
     2881 ggggcgaggg cacctattat ctgccgcata aattggccat ggggccgacc cagccgcata
     2941 gcttctgggc ggaaattttt tttgaaggca gggcgcactt ttcgctgttt ctgcgctatg
     3001 ccaacggatt gatgctctgc atgctgctgt atcttttggc cggcgcgctg caaaagcaga
     3061 gccgagacgt gctgtttgct gcgcggctct cagtctgcgg gctgtttttc tttctgttga
     3121 tatgggaggc gcgcagccgt tatctggtca attatgtgcc ggtgctgctc ctgctgtttg
     3181 ctgcggccgc gtggggaatg gccggtcgcc tgcctgggtg gggctgcaaa cagcgcggta
     3241 caggcggtga aagtataccc ggatgaagca tccgtgtttg gctggagcaa aggcaaacgc
     3301 cccgcgccgg ggtatcccgg ggcggggcgt tttttcaaaa agttcctcgc gcatatgc
//
LOCUS       DAWBSB010000241         3336 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_4699_length_3336_cov_1.936605, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000241 DAWBSB010000000
VERSION     DAWBSB010000241.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4699_length_3336_cov_1.936605"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    620..629
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1534..1543
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1836..1845
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2754..2763
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          711 a          980 c          915 g          690 t
ORIGIN      
        1 ggcacccaca tcatcgacat aagcgagaac gagatcgcgg tgccggagga ttacacccaa
       61 gaggagcagc gtccggatta tatatgttat accacaccca cgctgcccgc gccgcttacg
      121 gtaacaggcg acgcgcgcgt gacgctttat gtctccagcg atgcgccgga tacggatttt
      181 atggtacgcc tgtgtgatgt ggacgaagca ggccgcagcg ttaagctggc ggacggcatt
      241 ctcgccgcgc gtttccgcaa cgggttttcc gcgccggaat atctggagcc cggcaaggta
      301 gaaaaactgg tcatccgcac ctctaaaata tcccattgtt ttctgcccgg acaccgtctg
      361 cgggtgagcg tcacttcggg cgccaagcat tttgtgtttc ccaacagcaa tacgaaagat
      421 gcatacaaca gcacggaaag ccgtgtcgcg cacaacaccg tctggcacgg cggcgcagca
      481 ccgtctcata tcctgctgca cgttgaacgg tgaagcggcg ccctgcacac tgcgggacgg
      541 atgcgcaaaa ccgcatgcac cggtgcgatt tttaaaggga aggagggtct ccgccatgga
      601 aaccgagccg cttcagattn nnnnnnnnnt cttttccggc tttgaccgcg ccaacgaggg
      661 aagccgtatc caccgcaagc tgcaaaaagc ggcgggagat tcctataaag ccgaggtacc
      721 cttcaaggca gagcgcacgg tagacgacgt atgctatacg ctggagggac gtgccgacgg
      781 agttatcaca gagccggacg gacattttac catcgacgaa atcaaaacca caggcgcacc
      841 ggccgagctg ctgacggcgg attttaaccc cttacattgg gcgcaggcta aatgctacgg
      901 cgcgttcctg tgccagaggg aaagcctgcc tggcgtggac atccaaatca catattatca
      961 ggtcgacacg gatgagatca tccgccaccg ccgcacattt tccgccgaag cgcttgaaga
     1021 ttttctcacg gatacactgc gtttgtatac gccttgggcg cacatggagg cagcgtggag
     1081 agccacacgc aacgcctcgc tgaaagcact gcgcttcccc tttcccgcct accgccccgg
     1141 acagtacgag atggccggcg cggtgtaccg taccatcgcg ggcggcggca ggttgttttg
     1201 cgccgcgccc actggcatcg gaaaaacggt ttccactctt tttcccgctc taaaggcatt
     1261 gggtgaggaa aagggcgagc gcatttttta cttgacggca aaaaccatta caaggcaggc
     1321 cgccgaggat gcgctggcgc gtctgcgtgt tcacagcaca tcctgcggca caccgctgcg
     1381 tcttaaaacc atcacgctga cagccaaaga caagatctgc ccgctggagg aacgcgtctg
     1441 caccccggag gcctgtcccc gggcggacgg ctattacgac cgcatcaatg cggcgctgta
     1501 cagttttttg tcccaaaccg acgaattcac ccgnnnnnnn nnnctgcatc gtctgtgact
     1561 acaactacct gttcgaccct gtggtaagcc tcaagcgctt tttcaccgag ggcggagact
     1621 tcatttttct catcgacgaa gcgcacaatt tggtggatcg cgcgagggat atgtattccg
     1681 cacagctgct caaatccgcg gtttatggcg taaaaaaagc gctgggcaaa gggcaccgca
     1741 agctgacggc cgctctgaac aaagtcaaca gcgaaatgat cgagctgcgc cgccgctgtg
     1801 aggaagcgga tgaacatcgg ctggtcattc cggaannnnn nnnnngcttt acgacacgaa
     1861 ctacaccacg cttctcgctg ccttcggttc ggaggtctgt gtccgtttgc tttgtctgga
     1921 cgccgcgccg tttctggatg catccatggc gcttggccgc gccagcattc tgttcagcgc
     1981 cacgttatca ccggcggaat actttattca aacgctgggc ggcggcccgg ccgccaaagc
     2041 gatacgcctg caaagcccat tttctcagga aaggctgtgc ctcgccgctg ccgggatcag
     2101 cacaaaatat acagaccgtg agcgcacacg ggaatccgtg tgccgtctca tcggcgcggc
     2161 cgtgagcgcc catgcgggaa actatatcgt gtttctgcct tcctataaat acatgcagga
     2221 tgtgagcgac gaatttgcga tgatgtatcc tgatgtccca ttgaccgtgc aggaaaacgg
     2281 catggacgaa gatgcacgcg aggcatttct cgcacagttt tcagccgaca ggcccggaac
     2341 gcttgtgggc ttctgcgtgc ttggcggcat ttttgccgag ggcattgatc ttgcaggcga
     2401 ccgtcttatt ggcagcatca tcgtcggcgt gggcttgccg cagattggcg tagaacaaaa
     2461 tgccctgcgg gattactacg aagacacaat gggtatgggt tttgaatacg cctatcaata
     2521 tcccggcatg aacaaggtcc ttcaggctgc cggccgtgtt atccgtacgg aaacagaccg
     2581 cgggctcgtg ctgctcatcg acagccggta ccgaaccggc agctaccgcc ggttgttccc
     2641 cccgcactgg tctcatctgc ggcggataga gagcgcggaa cagctctccg cactgctggc
     2701 cgacttctgg gccgagccgc cttccaggca tgcttttccc aaatagcata caannnnnnn
     2761 nnntttttaa tcgtggaatc aaccaagcct tctgcaccta tccggcgcgt cacccctctg
     2821 cggcgtttgc cctgcgccgc aggatggtgc cctccggcag agcggctgtg cacggaatgc
     2881 tgaaccgcat catcgcatgc ggcgtagcac agattgcttc tccgctttca ttttgtatcc
     2941 attccggcgt aaaggcaaca accttgccgg atggcgcaag cgcttccagc gcctctccaa
     3001 gcaggaactt tccgcgttgg gtgcaaaaag cagttccttc gcgccattca tctacaacgc
     3061 ccacaacttc ccattcccga atgtagccgc cgtgctttgt attttgcgtc gcaccttctc
     3121 ggccgaaata aaaaccggtg gaataatgcc ggtgcgatgt acgggtcaat tcctccagca
     3181 cgtcttcggg gcacatgtaa gcgtccggcg catccagata tgcgttcacc gcacggcggt
     3241 atgcgcttgt ggtactggcc acgtaataga aggttttggc gcgcccttcg attttcaggc
     3301 tgtccacgcc cgcctgtaaa atcaagtcga taaacg
//
LOCUS       DAWBSB010000242         3333 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_4703_length_3333_cov_2.774253, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000242 DAWBSB010000000
VERSION     DAWBSB010000242.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3333)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4703_length_3333_cov_2.774253"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1835..1844
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          625 a          931 c          981 g          786 t
ORIGIN      
        1 taggcataat ccccgcggga tttgttcgtc atgttgtcga tgttcagccc cgccttgctg
       61 gtcacgtctg taatggcggc caggatgccc gggatattgc ggtgtaccac gcagatgcgg
      121 ctgccgcccg cacggggcat ggagaccgag ggaaagttca cactgttttt gatgttgccg
      181 ttcagcagat aatcccccag ctcatccgcg gccatcacgg cgcagttgtc ttcgctctcc
      241 ggtgtggagg cgcccaggtg cgggatgcac accgcgcctt ccgcggaaag cagcgcctcg
      301 ctggggaaat cgcacacata acaggccacg cggcccgttt tcagcgcgtt caccatggcg
      361 gccgtattca ccagctcgcc ccgggcaaag ttcaggatgc gcacgccgtg cttcatttgg
      421 gcaatgaggc tgtcgttcac aaagcctttt gtttcagccg tggcgggaat gtgcagcgtg
      481 atatagtcac actgggcgat gaggtcggac aaattgacgc agtggcgcac gctgcgcgaa
      541 agactccagg caccctccac agagatgtac gggtcgtacc ccagcacctc catgcccaga
      601 tgggtggcca tattggcgac cttcacgccg atggcgccca ggcccacgac gcccagggtc
      661 ttgcccgcaa gctccggccc ggtaaattgg cttttcccct tttccaccag cttgccgatg
      721 gcgtcgccct ggccggccag cgttttggcc cactccatgc ctttgcctac cttgcggctg
      781 gccagcacca gtccggccag taccagttcc ttcaccgcat tggcgttggc gcccggcgtg
      841 ttgaacacca cgatgcctgc ctcgctgcat ttgtccaccg ggatattgtt gacgcctgcg
      901 cccgcccgtg caatgcccag caggctttcc ggcagcgcca tatcctgcat ggcggcgctg
      961 cgcaccagaa tgccgtcggg ctgcgcgccc tcctcatcca cggcaaacag gttgctggaa
     1021 aggcgctcca gccccttgga cgaaatattg ttcagtgttt tgatattgta cattttcgtt
     1081 tccccctgtt ccggctgtgc gtcaggcgtt atccgcctcg aactgcttca tgaattccac
     1141 cagcttttcc acgccttcgg cgggcatggc attataaatg gaagcgcgca tgccgcccac
     1201 gctgcggtgg cccttcaggt tgacaaagcc cgcggccgcg gcttcggcgc agaatttttt
     1261 gtccagctcc gggctggggc tggtgaacgt gacgttcata atgctgcggt ccggcttgtt
     1321 cacgggattg ctgaagaaag acgagccgtc cagataatcg tacagcagct tggccttggc
     1381 gttgttgcgg gcctccatgt tggcaaggcc gcccacttca gtttcgatcc atttcagcac
     1441 aaggcccatc atatagatgg tgtagcaggg cggcgtgttg tacatgctct cggcgtcgat
     1501 gagcgttttg taatccagca tggcgggccg ggcgatatcc gtgcgggcgt tttccatcag
     1561 gtcttcccgg atgatggcta cagccatgcc cgcgggcgcg acatttttct gcacgccgaa
     1621 gtagatcagc ccgtattggg agacgtctgt gggacggctg aggatgcagc tggatagatc
     1681 ggccaccagc ggcacaccgt ccgcatgggg cagctttgta aagcgcgtgc cgtaaatggt
     1741 gttgttttca caaatgtgga tatagctggc gtttttggaa tattcgacgg cgtcaacgtc
     1801 cggaatatag gtgaagtttt tatccttgga cgagnnnnnn nnnncgggca cattcaccct
     1861 gccgaatttt tcggcctcct tcgcggcctt gccggagaac tggcccgtta caagataatc
     1921 ggcctcgccg gtggtcatca ggttcatggg caccatggcg aactgtgtgg acgcgccgcc
     1981 ttgaatgaag cccaccttat aattatccgg aatacagagc acgcggcgca gagcggcctc
     2041 ggtctccttg atgatggcgt cgtacacaga actgcggtgg ctcatctcca tcacgctctg
     2101 gccggatgac ccgtactcaa gcatctctgc ctgagcggtt ctcaggacgg attcgggcag
     2161 catggatggg cccgcgctga aattgtatac tcgtgccata attccttttc ctccaaaata
     2221 caaaatcact ttatcaaaat aaagtgtata gtatttatta taaaggctgc cgcgcggttg
     2281 tcaatgcaca aaaatgcccg tctgcacgcg gcagacgggc aaaaattgca catattgacg
     2341 gtttttgaat aaaatgtatt cgagagaagg acaaaatgct cattcaccgg ctgcggcgga
     2401 agcggcgctc ggcgctgagg gagccacggg tgttgaggga gcggaggcgc ttttgccgga
     2461 gccggctgcg gcgatgatat cggaaatatc gaacatcatc gagccgtcgg aagcgacgct
     2521 gggcagcttg ccgtcccatt tgttgatcca ttccatggca agtgtttcct gggtgatctc
     2581 ctggtttttc agccggtagc tctcggcctc ggcacgggcc tgttcgatct gctgctgagc
     2641 ttccactttg atgcgggcca ggtcctgctc ggctttcagc gcgttctgct gggcggtctg
     2701 cttggcctcg atggcggcgt tgaattcctc gctgaactca aagctggtga tgttgaatac
     2761 ctcgatgtcg aagccatagg ggccgatttt ttcggccagc gcctcgcgca tcaggtcgcc
     2821 taccttctgg cgttcggtga tgagctcctc ggcagtgaac tgggcggtga cgctttttac
     2881 gcattcctga atggcgggat tgatgagcac attttcaaaa tccgtgccga tgttctgata
     2941 aatgctggcc gaattggcgc ggccgatgcg gtagttcagc gcaatggtac tgttgacagt
     3001 ctgcagatcc ttactggcgg agctgctgga tacctcggtt ttgagcacgc ggttgtcgat
     3061 tttgacaaca ttggtgatga acggcaattt gaaatgcagg ccctcgccca gcacggtgcc
     3121 gctcaccttt ccgaaggtgg tgacgacgcc ggtgtggccc gcgggaatgg tcgtgacgct
     3181 ggagagcagc agaaacacgg cgagaatgac tgcgagcagg atggggatga atcggaattt
     3241 ttttacttct ttcatgtttt tctccttttt aaataatggg aagcttgttg ctgagggaag
     3301 taaactgaat ctctcggcgc atgcgggtca gcg
//
LOCUS       DAWBSB010000243         3330 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_4709_length_3330_cov_2.005191, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000243 DAWBSB010000000
VERSION     DAWBSB010000243.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3330)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4709_length_3330_cov_2.005191"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          682 a          844 c          994 g          810 t
ORIGIN      
        1 gcgcgtgacg gataacggcc cggggatacc gccggaacgg ctggccatgc tgcaggcaga
       61 ccttgcgcgc ggcctttccg tcagcgggga ggggtacggc attttcaacg tcaacgaacg
      121 tatcaggctg tattttggcg cagagtacgg attgatgctg gagagcgaat atgggcgcgg
      181 cacggtggcc gccgtgcgat tgccctgtat cacacaggat gaggtggagc aatatgtatc
      241 gcttactgat agcggacgat gaggagagca tccgcgaggg cgtggcggat tttgtgcggc
      301 agaactgccc ggaatgggat gttgcagcac tggcgcggga cggacgcgag gcgctggcgc
      361 tggcgcggga gattctgccc gatgcggtgt tgacggatat cacgatgccc cacatgaacg
      421 gattggagtt tttggaaagc ctgtctgatc ttctgccgga ggcaaagctg ctggtgctga
      481 gcgggtatga ccagtttgaa tacgctgtgc aggctctccg tcttggggtc agcgattacc
      541 tgctgaagcc gttggacact gcaaagctcg tttccgcttt gagccgtttc gcagcggaac
      601 tggacgcaca ggctttgcgc tgggcccaga tagaaacgct gcggaccaac acgcagaaaa
      661 ccaacgagct ggaattgcag agctatttcc gtgctgcgtt gctgggagaa gagctgcctg
      721 ctctctctgc ggcaaacgcc gtgtttgcgc aagaggggac aagctattgc tgcgtactgt
      781 gcgacggcct ggacgcacag cgcgctcttt tagagcgggt gctggaacaa cggctctatg
      841 gcgcggtgcg tactgtactg ctccgtctcg gcacgccgcc ccggcaggcg gtggtgttct
      901 gtgcgccccg cacggcacgc gcagggctgt ttctcacgct gagccatgcg cttacttcca
      961 ttgcggtatc ctgtaaacgc gcgcaggcgt tggatgtgca tttttttgtc ggctgtattg
     1021 cggaaacgcc cgccaagctg gagctttcct accgccaatc tacgcaggct cgcgcctatg
     1081 cgttcccgga acaggcggcg cccgttacca cctacgagga tgttttggcc ggaaagcttc
     1141 tgccgtgtcc ggagccgccg gaggagctcc ttcgtgacat ccccgccgcg gtacagtgcg
     1201 gcagccgggc tgcttttgtc caaaattgcg aagcgctgtt cgcatggttt gtggagcggg
     1261 agatccgcga tgcgacattc attcgcatgt gcgtgctgcg cttgtgctat tccattctga
     1321 aaaaaccagc ttccgatatg ccgatgagct attacgaatt cacaaatttt cagcaggaga
     1381 tcatggcggc ggcgagcatg gaggagctga gagcctgctt cgagaacttc gtatcgctgt
     1441 actggctgcg ccgccaggga gaaaaaccgc cccggcgcat actgacggag cgggtgagcg
     1501 aggtggtgca ggcgcatatc tcagacattg atttttctct ggatgacgtg gctgcggcac
     1561 tgtttatcag ccccaattat ctgcggcagc tgttcaagca ggagactggg cagacgttta
     1621 cggaattcct gacagcgcag cgtatgcagc acgcacacat gctgcttggc aatccgcaga
     1681 cgaaggtgtg cgatgttgcc gaacaggcgg gctacgcgga ttcacgatat ttttccgtat
     1741 gttttaaaaa attctaccat atgacgccca gtgaatatca agctgccgcc ctggcgggaa
     1801 aggcttgata ttcgttgaat tttagacata tcttccgatg attttcctcc ctcgcatact
     1861 tgggccggcg gtataattga ggtaccaatt acggcaaggg gagtgacggc atgtcgaaga
     1921 aagggaaaaa ggtctgggag cgcgtctata acgagcgcac ctcgtatctg atgctgctgc
     1981 cgaacatcat catgttcgca gtgttcacgg tgtatcccat cctgtgggcg ctgcggtata
     2041 tgctgttcga ctatcgcggc tacggcgagg cgaagtttgt tggaatggac aattttgtgc
     2101 gtgtttttac gagggaccct gtgttttggg attctgtggt aaacaccttc gtctatgtgg
     2161 ggggcaaact cattcttacg ctgcccatcg cgttcatgct ggcatttctg ctcagtaaaa
     2221 gcacacgcat caacgccgtg agccagtcca ttatttttac gccaaccatt atgtccagcg
     2281 cagtgatggc tcttctcttc tatctgctgt tcaatacata caacggcgag atcaaccgtt
     2341 ttctgatgtg gcttggcgtc atcgatgaaa atataaactg gctgggggtg cagcatgcga
     2401 tgctcacagt catcatcctg gctgtttggg gtggtgtggg caactacatg gtgtatttta
     2461 tcgcaggcct gacggggatc tctacggatg tatatgaaag cgcaaagctg gacggtgcaa
     2521 acggtgcaca gactttgttc cgcatcacga taccgctcct ggcaccggtt ctgaagatga
     2581 tcttgatgct ggcactggtg atttccttta tggatatgca gagcatcatg gtgctgaccg
     2641 agggcggtcc aatgggcaaa acaaatgtca tgttccttta tatctaccag ctcttcttcc
     2701 cgatttccgc aggctctacg gtcacccagg aatttggata cggcgcggct gtcagcctgg
     2761 tggcggcttg catcatcggt ttgatcaccg gtctgtatct ctttatcggc agtaagctgg
     2821 acaaagtaac gggcgaataa ggagggaccg ctatggaaaa aacacaccgt ttgcgctggg
     2881 cagagatgcg ccccagcgaa cgccgcgatg cggcggtatc tttcatcggg caggtgctca
     2941 aatgggcttt tgtgggcgca atgtgcattt ttacgctgta tcctgttatt tacactctgt
     3001 tgggctcttt caaaacaaat gccgagctta cgctgggcgg cggtatcttg ccggaaaaat
     3061 ggcagattga aaactatatc tacgcattcc agaagctgga gttcggtaaa tatacgctaa
     3121 acagtgttgt gctggccctg ctgaccgtgt tgttcacagt tacgactgct tcgatggctg
     3181 catatatcat tgcccgcaga gagttcccgg gcaaaaaact gctcaccaca agttatctgc
     3241 tcagcatgtt catctccgtg ggttctgtgg cgctgtatcc gctgtacggc cttctgcatg
     3301 ccgtcggact caccagtaat atggtggggc
//
LOCUS       DAWBSB010000244         3318 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_4727_length_3318_cov_2.191235, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000244 DAWBSB010000000
VERSION     DAWBSB010000244.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3318)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4727_length_3318_cov_2.191235"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2374..2383
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          698 a         1031 c          866 g          713 t
ORIGIN      
        1 ccgtgacgtc gccgtcctga gccttgccct cacgggtaat gatgcgggcg ttgcgctcac
       61 gcatgcgctc gatctcggcc tccacagcgg cgtcctcgac agtattgact ttctttgtgg
      121 cttttaaacc cttgtattcg cccaccttca tctcgggctt gacagtgacg acagccttga
      181 gcacgacgcc gtcttcggtg ctggcggaaa cgatgtccac ctcggggcgg tccaccggct
      241 cgatgccgct ctcggcaacg gcagcttcat acgcctcggg gaacaggtca ttgatggcgt
      301 cgtagtggaa cacatcggca ccgaacatct tttcaatcat atggcgaggg gctttgccct
      361 tgcggaagcc cggaatgttg tatttaccgg cctcgcgttt gaacgctttg gcaattgcgg
      421 cgttgaacgc ttcggcgtca atggagaatt gcagttcatg gctgttgttt tcaagttttt
      481 cgtttttgac gagattcatt gtatttcctc caatactgaa cttacagagt acaattatag
      541 catacgttta tgaatatttc tatacaaatt caaattttat atggaaaaaa ccgcagtgcg
      601 tcggcactga tgagtttata ctcgatgccg tccagctccc cctgcaccgc ccgcggaatg
      661 gacgggccgt gcaaatggcc gtaaaagcag cgtttcacgc cgtattcccg caggagcgcc
      721 accacttccc cggcgtccgc ctggggatag accggaggat aatgcaggaa cactgccttt
      781 tccgtatctc ccgccgcttc caacgaagcc cgcagccgtc cgcattcgcg gctcattacc
      841 ttttcatcgt gaggctggcc cgcgtcgaac agccagctcc gcgtgccgca cagtgcaagc
      901 ccttctgcaa aaacacaatt gttatgcaga aaagacaggc tgtcaaaccc attcgccgcc
      961 agataggcgt tcattttcgc catggtcgtc caccagtaat cgtggttccc cttcaagagc
     1021 agtttcctgc ccggaagatt ctgaaggaac gcaaaatccg cactgcagtc ctccagcttc
     1081 atcgcccagg aggtgtcccc cgccagtacc acggtatcct gcggacccac agccgccagc
     1141 cagttttccc gcagacgctc cacatagttc tcccagccgc tgaaaatgtc catgggcttg
     1201 gacgttccca gcgacagatg aagatccccg attgtaaaaa ttgccatagc atcctccgaa
     1261 tgactggccg cagaccggcc atactaagaa aaggaagcgc cgcgccctat gaagcggcgt
     1321 tttgcgaact gattttaagg aggcaataaa aatgcaggac gaaaagattc tggaaggcat
     1381 ttgctgcgaa gtagagaact gtgtgcacaa caatggcaac tgctgctgca ccgcgcagga
     1441 gatcaaagtg aaaaaccgca tggccgtcct cggtgaagag accatgtgcg agacctttga
     1501 ggaagtttag gcttcgtcga aaaatgtccg caaaaacggc tcgtgtgata tgcacgggcc
     1561 gttttcattg cacccttttt ctctacaaag caagcgccgc agcccgaatg ccgtcctttt
     1621 ccaccacctg cgtgaagcgt acgggctttt cccgcaggcc cgcaaggctc gcaggcgccg
     1681 ggaccccggt cttttcctgc agcaggcgca tggcctcaaa ctcatcctcc ggcacatccg
     1741 cccccagcgc gcccagcaca tcgcacggga acttgtacgg gcttgccgtg gaaagtacaa
     1801 cgcaaggcgt ttcgccggcc gcctgctgcc gtgccacacg gaacgccacc gccgtgtgcg
     1861 tgtcgcacag gtatccgtcc gcttcatacc gcgcgcggat ctcctcccgc acctgcgcgt
     1921 catcggcaca gccgcaggca aaggtctccc ggatggcgga aagcacttcg gcgggcacgg
     1981 tgtaatgccc atgctccgcc agctcacgca tccatcccgc caccgctgcc gcatcctgtg
     2041 ttacatggta caaaaggcgc tccaggttcg aggacacaag aatgtccatg gacggcgagg
     2101 acgttttgta gaacgtgcgg cgggcgtcgt aaacacccgt ggtgagaaag tccgtgagaa
     2161 cgttgttttt attggacgcg cagatcagct tttccacagg caggcccata cgtttggcgt
     2221 aatagcccgc aaggatatcg ccgaaattgc ctgtgggcac acagaagctg acgggcgcgc
     2281 ccggtgcgat atccccccgc tccgcaagct gcgcatagct gtaaaaatag taaacgatct
     2341 gcggtacaag gcggccccag ttgatggagt ttgnnnnnnn nnnttggcag ccagctcctg
     2401 cgcgaggtct tcgtccgcaa acacgcgctt tacgcctgtc tgcgcgtcgt cgaagtttcc
     2461 gttgacagca tacaccgcca cgttatctcc ctcctgcgtg gacatctgca ggcgctgcac
     2521 ctcactggtg cccgcgttgg gataaaacac cgcgatacgg atgcccggca ggcccgcata
     2581 gcccgcgagc gcggcctttc ccgtatcgcc ggaggtcgct acaaggatct ttgtcacgcc
     2641 tgtttctccc agattttttt tggcctctac cagcagacgc ggcataagct gcaatgcata
     2701 atccttaaac gcgcaggtcg ggccgtgcca caactccaat gcataggtat tcccggccac
     2761 atgcgccgca aagcctgcct tgccgccgaa gttttcggtg ctgtatgtct gctctgtggc
     2821 ggtttttaaa aaatccgggt cataatccgt cagaaacatg cccagcacct tctgcgccgt
     2881 ctgcggatac gtcaggccgt aaaactcccg catgtccacc tgtgggaact gctgcgggac
     2941 aaacagcccg cctcccggcg caatgcccgt gagaatggcc gtggacgcac cggtgcagag
     3001 agcactgtcg cgcgtgcttg tgtagttcat actttacccc tccaattata gccacagccg
     3061 ctatccggct gttccgccgt tttccgcagc cctgcaaggc ctgctctttt tccgtgggac
     3121 agagcggctc tgcccagccc tttctaaaaa gtgcgcttcc aaaagcccca ggcgttttca
     3181 aaaaacacct tgttcaaaac cgtctccggc agcccgcgcg ccagaaggta cccgtataag
     3241 cgcgggatgt ccccgatatc ccggatatcc tccggcacat gtgcgccgtc aaagtccgtt
     3301 ccaaagcaca ccgtatcc
//
LOCUS       DAWBSB010000245         3281 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_4796_length_3281_cov_2.570676, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000245 DAWBSB010000000
VERSION     DAWBSB010000245.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3281)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4796_length_3281_cov_2.570676"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          667 a          898 c         1089 g          627 t
ORIGIN      
        1 gatgaagcgg agctggacga cgatgaataa gcagcgttat ctgctggtgg tttctggtcc
       61 gtcgggcgcg ggtaaggata ccgtcgtcag ccggatgatg aaaaagcatc cggaaatcga
      121 gatatccgtc tcggctacca cccgcgcgcc ccgtgcgggt gagacggatg gtgaaaatta
      181 ttattttctt accaatgagc agtttgagga aaaaatcgac cggggcgaga tgctggaata
      241 tgtgaattac tgcgggaagt attacggcac acccaagtcg gaggtggacg cccgcttgga
      301 caggggcacg accgttgtgc tggtcattga ggtggtcggc gcggcgaata tcaaaaagct
      361 ttatccggaa agcacgctca ttttcatcat gccgcccagt atggacgagc tgcgcgcccg
      421 cctgcgcggc cgcgggacag agagcgaaga ggcgattgcc ggccggctca agcgggcaga
      481 ggaagaactg ccctatgcca gagagtatga tttttcggtg gagaaccgcg atgtggacgc
      541 atgcgcggac gagatctacc gtattttgaa gcagcgccag caggactagc gttatgtaaa
      601 aggaacaggc agccggaaag ggagggtgga gtgcttggcc gaatgcgtgc aggtggcggt
      661 cagcaacgcc acctttcatt ttgataagct gtattcttac agtgtgccgg cgcacctgca
      721 ggacagagtt tttgtgggca gcatggtgct tgtcccgttc ggccgcggcg cggccgcgag
      781 gatgggcgtg gtgctcggac tggatgcgcc ggaggcggcg ggcaaaaggc tgaaatcact
      841 gtacgacgct gcgcctgaac aggcgcgcct gtcggaggag ctgctgcgtc tggtgtatta
      901 tctcaaggaa cacacgttct gcacctatta cgaggcggtc aaggccatca ttccctacgg
      961 agcgcagtac aaagctgtga ataccggccc tgtaccgcag ctgcaaaagc agcttgtgcg
     1021 ccataccgag ccggtatacc ggcgcacaga cgccccctgg caggggcgcc gtaccgaaaa
     1081 gcagcaggct gcctgggacg cgctggcttt cggccccaaa acacagaagg agctggaaga
     1141 gcagggcgtg acgaaggcgg tgctggcggg ccttgtgaaa aaaggcgtgc tggctgcttc
     1201 cgctcaggag aatagcgtag cgttgtatga gaattacagc cccgcgcagg agacgatcgc
     1261 gctttcaccg gcgcagcagg cggctttcag ccgcctggcg gctctgatgg atacgggccg
     1321 gcccgcggcc gcgcttctgc acggcgtcac ttccagcgga aagacgctgg tgtttttaaa
     1381 gctcatcgaa aaggcggttg cggcggggcg caaggcgttg gtgctggtgc cggaaatttc
     1441 gcttacgccg cagatgatct accgcttgaa agcctttttt ggagacagag tggccgtgca
     1501 gcactcggcg ctctccaata ccgagcgcct tctgcagtgg cagcagatcc aaaacggcgg
     1561 cgcagacatc gtggtgggca cgcgcagcgc tgtgtttgcg cccctgccgg acatcggcgt
     1621 tattatcatc gacgaagagc aggagcatac ctatcactcc gagagcgcgc cgcgctattc
     1681 cgcccacgac gtggcgaagc gccgtgccgc ggctcacggc gcgctgctgc tgctggcctc
     1741 ggccacgccc tccacggaaa gctactatgc cgcgcaggcg ggccggtata cgctggtgga
     1801 gctgtcggaa cgctacaacc acatgccgct gccggaggtg cggctggtgg acatgcgcga
     1861 ggaactggcc gcgggcaacg catcgtccgt cagccgggcg ctggcggatg aaatttcgcg
     1921 caatctggcc cggggcgagc agaccatcct tcttttgaac cgtcgcggat acaaaacagt
     1981 gggcatgtgc accgcctgca gtgaggtggt gaaatgcgat gcctgcagcg tgcccatggt
     2041 gtaccacaaa gcggacggcc gtttgctgtg ccattattgc ggcaaatccg tcagtccggt
     2101 gccggaggtg tgccctgcct gcggcggcaa gctgaagtat acgggcttcg gcacccagcg
     2161 cgtggaggag gaattggcgc agatgtttcc cgcggcgcgg gtgctgcgca tggacctgga
     2221 cacgacctcc cgcaaaaacg cccacgagac catgctgcgc cgcttcgcaa agggcgaata
     2281 tgatatcatg ctgggcacgc agatggtggc caagggactc gattttgaaa aggtgacgct
     2341 ggtgggcgtg ctgggcatcg accagctttt gtttgcgcag ggatacaagg cgttcgaaaa
     2401 tgttttttcg ctggtgaccc aggtggtggg gcggggcggc cgcgcggctc aggcgggccg
     2461 ggcgctcatc cagacggtgg acccaaacca tccggttctg aatctggcgg cgcggcagga
     2521 ttataaatct ttttttgcag aggagatatc cttccgcagg ctgaaccttt acccaccgtt
     2581 ctgcaccatc tgtctggcgg gcttttccgg tgcgaaggag ccggaggtgc tggcggcatc
     2641 ccgtgccttt gcggcggacg tgcagcgtct tgcacagcag cgcagcggta ttccgctgcg
     2701 tatcctcggc cccgcgccca tgaacattgc catggtgaac aaccattacc gctacaagct
     2761 cacgctcaag tgccgcaacg acaatgcttt ccgcgcgctg atgggcgagg cgctggcggc
     2821 gtacagcgcg gccggatggc cgggaaaagt gtccgtttat ctggatttcc attcggacgc
     2881 agacatttaa cacataaagt attatcagct gcaaacgaaa atcttggagg gatttgatta
     2941 tggctttacg cactatcgta caggacggcg atccaatctt gaaaaagaaa tgccgtcccg
     3001 ttacaaactt tgacgaccgt ctggcggagc tgctggacga catgatcgag acgctggccg
     3061 acgcacaggg gtacggcctg gcgggcccgc aggtgggagt gatgcgccgc gtgttcgtca
     3121 gcctggatga gcgtgacctg cctgaggacg gagagctgcc cgagggttac gagccgaagt
     3181 acctggaatt cgtgaatccg gagatcatca tgcaggaggg cgaggtgctc cagtatgagg
     3241 gctgcctttc gtttccgggg cataacgccg ccatccgccg t
//
LOCUS       DAWBSB010000246         3245 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_4864_length_3245_cov_1.921003, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000246 DAWBSB010000000
VERSION     DAWBSB010000246.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 3245)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3245)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3245
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4864_length_3245_cov_1.921003"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2665..2674
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          785 a          835 c         1033 g          582 t
ORIGIN      
        1 gcggtgctcg ccgtagaaat gagcgccgtg cgcccggtgt tccagcctgt gccgggtaag
       61 gggaatttct tctacgagga gcttgccaag acgggcgcca gcgagaaagg ccagattttc
      121 ggccagttcg gcctggacca cggcccagcg ttcctgcacg gctccatcac cggcctgacc
      181 tgataggagg gaacagatat gacgcccgat gaaatcatga aaatggaagg aattgcgccg
      241 gagctgcgca aggtgcttgc agacatggct tccgacacca cggcgcttga tgcgcgtgtt
      301 gccgcactgg aggaagccgc ggagcagggc gcgtaaggga ggctgatgtg cgatgaagcc
      361 cgaagaaaag gaaaagcagt tggacatcat ccgacgccag ttgggattgt ccgaagcggg
      421 cgaggagctt ggcgacctgt tcgacgggct tctcgcctcc gccctgatgc acggcaaccg
      481 caccagtacc ccggatgcgc tgctgaagct ggtgcgggac gctgtatgcg cagcgtggcg
      541 ccgccgcggc gatgagggca gcaccggcag cacggcgggc ggccagagct atacctacga
      601 ggatattgaa gctactctgc acaaaaacat cgtggcagca ggattgcggg tgctgcggct
      661 atgaaactgg aaaacaacag caccgtgtgg gtgacgacgc cccataccgc gcggatcaga
      721 gcgcaggagg agaccgtata cacccgcctg cgtgcggtgg agatcaatgt tcagactgac
      781 cgctccgaag ttgatgtcca ggcgtacggc aagaccgtga acgagcttat aaagctgcgc
      841 agcgaaacgc cgccgaacat aggtaaggat gaccgcctgt acctgacgcg gcctgcggta
      901 cagcgcaccg tcgagataca cggggaagcc ctggaggatt acgggcaggg agaataccgt
      961 gtagaggccg tgcgcagcgc gttcataggc ggcatacatc ccaaaaaccc aacgctgata
     1021 gaggcggtgc gtgtgaaatg gtgaccttcg atttcaaacg tacagggccg ggactgcagc
     1081 gtattatcga cggcttgccg ggagcggcgg agcgcggtgt gcggcgcgct ttggacgaca
     1141 cggcgaagct ggccatccgg atggctccaa atgtcgtcgg gaagtgcatc aaggtcgaga
     1201 tgctgaacag cgagaccgga gaaatcgtcg gacgcgtata taccgacacc ggggttacga
     1261 gttttgcgcc atacgtggaa tttggaacag gcgtcaaagt ggatgaccag ggcaacccgg
     1321 aggccatacg cctcaagcgt gccaagcaaa ttccatggtt tatccatgta agcatggtgc
     1381 cgcgcagttt cgcaaaatac ggatatcctc ttatcgtggg gcgcgacggg gcgcaatact
     1441 gggttgtaga cggcatgaag ccgcatccgt atttccatcc ggcggcgttc aaccgtcgag
     1501 gcgacaatgt aaatgctgtg aagcaagaga ttatcgccct tgtgaccgga ggaggtgacg
     1561 tatcggatga atgacccgct gcacacgttg aatcaggcgg agattgccgt gatgttctgg
     1621 cagacagtgg tggattcagg attgttcccg gacgaagaaa gcgtgatgct ggaaaatccc
     1681 agcacccgcg ccgtatttcc gtgctgcacg ctctccgtgc cgctggcgcg ccctctgtat
     1741 atggggaggg cctacgatat ctctatcacg gttgaggtgt gggcggacaa gcagcttgac
     1801 gccgtgcgcc tgtttgaaca gctccgcgca gagctggaaa agctgaacct gcggcagacc
     1861 gggaacgtgc ccagccagca ggacgatatc acagaaaact ggcgtttcgg agggtacttt
     1921 gaggtccgct ggaacgccat tgacaacaca tttgaaagaa acaactgaac tggagggata
     1981 tactttggca aaatacaatg cagaatttac gcctccgtcc gcaactgtgc tgaccgagct
     2041 ttattatgcg gaaacggaca ccgttgcacc tacgcaggtg ttcggcgtac agggcatccc
     2101 ggaggtggac actccgcccg atgatatcac gtaccgcacg ctggaaagcg acgttgagtt
     2161 cggcgtcccg ggcgtgaaac cgttcagcgc catcgaggtg gagattctct actacaaaga
     2221 gcagtacacg acactgaaag ccctggaggg caaaaacctg ttctggtatg taaaactgcc
     2281 agacaacacc gccggaagca cgcccgcggc aaaatcactc gtgaaaaaat ggaagggaga
     2341 tttccgcatt actctggcgg cgctggagct ggacgacatg gtgaaatgca tgctgaagat
     2401 ctacaaggcc accgcgccag aagacctgac gaccggactt cccaccggag gcggctgatt
     2461 cccgatggac tacctgacgg tagtccgcag catatgcttg caaccgtatg ctgcggagtg
     2521 ccgtttcggc ctgaaaatac acaacgatct ggaggaaatg tacatgctta tcgtaacgaa
     2581 agaaaaagaa gtggaactga aactgaaaac acgcctgatg ctgctcctgg aggaacggct
     2641 gcacatcgag gacacgcagc ttttnnnnnn nnnncaggcg gggaatatcc gtgtcctgac
     2701 cacggcggta ctgattcttt ccgacggtgc tctgaagaag ctggaggatg ctgcggacct
     2761 gctggacgca tatctggagg aaaacagtga ggcgtcggtg cagaatttgt acgttgccct
     2821 tttgacggag atcaatgaac gcggtttttt cggccggaag atgacgaagg agcggctgaa
     2881 ggcatggctg gaggagccgg cgctggagtt gggcgagatc gtcaaccgcg cggtggacaa
     2941 ggtggccgag ggcatggtgg cctccgaact tggcgcgcgg taattgacgc cagccgggcg
     3001 gccgcttatg catgcggtat atccccgctg gaatttgggg aaatgacgca cagggagata
     3061 ggcgaatatg tgcaggcaca gaacgaccga caggaagagg acttcaagaa acaggcgatc
     3121 ctgctggatg ggctgtaccg gcaatggata tccacgcagc agaaaaaacc gagagtgctc
     3181 aacctgaaag aactgtatcc gagcctgttt gggaaaaagt ctcccaagca tatgacgccg
     3241 gaaga
//
LOCUS       DAWBSB010000247         3221 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_4906_length_3221_cov_2.950411, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000247 DAWBSB010000000
VERSION     DAWBSB010000247.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3221)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4906_length_3221_cov_2.950411"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    281..290
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    997..1006
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          680 a          868 c          945 g          708 t
ORIGIN      
        1 ccttgccggt atactccatg gtaatgtcgt tttccgtgac ggtgaccatc gcgtaaatgc
       61 cgtctttctg gatgtaatgg gcctcgggaa tggcgaaggg cgtttcttta ttgagaaact
      121 cgctttcaaa gatgccgtaa acatccttgg ggttcagctc cttgtgcaga tggtcgctga
      181 catccttcac cgcgtagccc agcgcctcgc gcatcttggg cggcaggtcg tagccgtaat
      241 tctgctccag gatatatccg atgccgccct tgccgctctg nnnnnnnnnn ggctgttgat
      301 gcggatgacg tccgtttcat attcgcggcc cacgtcgtgg ggggcgatgg gaagataggg
      361 gacggtccat tcgtaaagat ttttctcctc acgccatttc atacccttgg cgatggcgtc
      421 ctgatggctg ccgctgaacg ctgcgaacac cagcgctccg gcgtaggggg cgcgctcgta
      481 tacatgcatg cgcgtgacct tttcgtattc ggcaacaatg gcgggcatgt ctgaaaaatc
      541 cagcccggga tccacgccgt ggctgtacat attcatggcg acggtaacga tgtccacgtt
      601 gccggtgcgc tcgccgttgc cgaacagcgt gccctccacg cggtccgcgc cggccagcag
      661 gcccagttcg gtgtcggcca cgccgcagcc ccggtcgttg tgcgggtgca ggctgaggat
      721 gacgtgctca cgatacttca ggttgtcgct catgtattcg atctggttgg catacacatg
      781 gctcatgctc atggccacgg tgacgggcag gttgatgata acgggctttt ccgccgtggg
      841 ctccagaatg tccagcaccg cgttgcagac ctccagcgcg tattccggtt cggtgcccgt
      901 gaagctttcg gggctgtatt caaagcggaa attgccctcg tactcggccg cgagctgctt
      961 gacgagcgcc gcaccgtccg tggcgatctt cttgatnnnn nnnnnnggtg ccgaacacca
     1021 ccttgcgctg gagcgtggag gtggaattgt agaaatgcac caccgcgctg ggcgcgccct
     1081 tgagggcgtc gaaggttctg cggatgatat ggtcgcggca ctgggtgagc acctgcaccg
     1141 tcacgtcgtc cgggatgagg tgctgttcga tgagcgcgcg cagaaattcg tactccgttt
     1201 cgctggcggc ggggaacccg acctcgatct ccttgaagcc cagcttgacg agaaaacgga
     1261 aaaaatccag cttttcctcc aggctcatgg gtacgataag gctctggttg ccgtcgcgca
     1321 gatccacgct gcaccagatg ggcggttttt caatgtggtc tttttttgcc cagcgcattt
     1381 cgcaccgggg ggcgggaaaa tatccgggtt tgtacttgtc tgcatgcatc atggttccat
     1441 tctccttttc ctgttcggtt ttgggggatg gccatgggaa cgaaaaaagc tccgtctcat
     1501 ggccgtttgg gccaagagac ggagctttaa aaatcagctt cgcggtacca ctcttgttgc
     1561 cgggaaacac ccggccgctt cgtgccatca gcctgcaaaa caggcgaaca gcctgacatg
     1621 ataacggtgt caaacccggc tgcgcctact ttgcgaatgc atttcagctt gctgctcggg
     1681 ggccagtacg catcacgccc ggcgctgttt ttcaccagcc aacagctctc tgcggctcgg
     1741 gactgtgtgc ggtttccccg tcgttgcatt ttttcatatt gctattattt taatggcccg
     1801 cgcgaggttt gtcaagagtt ttttagatga gaagtttgat atatcagcac ctataaaagc
     1861 tctttaatga gtgcaaacca atatacttga ttgtaagtgg cggcaatagc gtcttttgaa
     1921 acagattctc cccgttcacg gcgaagaaaa attgggtgga tacgaaggaa ttcttcaaat
     1981 gtgagcctgc gaggagaaga tatatggcat gagggaatat gcgattgggc attgcttaca
     2041 aaaatgaaat tgttttttgc gcatgcgcaa aaccagagag gcgtacgttg agaacgtgga
     2101 acagtatgaa attcacaagg gtggaaagag tatggagcat atgcttccaa aggctataca
     2161 tttgattgtc agtcactttt gtcacctcgg cttcagccta cattgatgtg cgcctggggc
     2221 tgtacggccc ggcgtgctgc agcactgtcc ttgcgggcgg aaaggctgat ggccagcgaa
     2281 accggcccca cgcggccgat gaacatcgtc agcatcgtca ccacgcgggc aaaggggttc
     2341 atcaacggcg tcacacccac tgaaagcccc acggtgccga aggcagacac agcttcaaac
     2401 acgctgttca cctcgttcag ctccggcccg gtattgtaga acagcgtcat ggaggcagca
     2461 atcacagcga acagagagag catcaggatg gtgagcgatt tatatacggt tttatggtcg
     2521 atgcggcggc cgaagatcat ggcctcgtcc cggccgcgga cgacgctggc cacggccaga
     2581 agcaccacgc tgagcgtggt gaccttgatg ccgccgcccg tgccgcccgg cgccgcgccg
     2641 atgaacatga gcagagacat gaagatcttg gtgaaattcg tcatatcccc cagcgggatg
     2701 ctgttgtagc ccgctgtgcg ggcggagaca gactggaacg ccgcgttggt gatcttgtcg
     2761 aacaggttca tcggccccag cgtgagcgga ttgttatact ccatgaacag gatgcccgca
     2821 gtgcccagca ggagcagcca gccggtgatc cacaaaacca gctttgtgtg tgtgcgcagg
     2881 tggtgtgtac ggtggtaggc cgaaaggtcc tgccagacaa gaaagcccag tccgccgctg
     2941 atgatgagca gtgaaataac agccaaaacg tacggattgc tctgataagg aataacgctg
     3001 ccgcccggca tgacgcggcc caggatatcg aaccccgcgt tgcagaacga cgagatggaa
     3061 atgaacaccg agatgaaaat accatccgca ccgaactgag ggacgaacac aagagcaagc
     3121 agcgccgcac cgatggcctc gaaaacaaag gcgaccttca taacgaactg cagcagcgcc
     3181 cgggcctggc tggcgtccga aaggttgatg ctctcgctgg c
//
LOCUS       DAWBSB010000248         3214 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_4915_length_3214_cov_2.253878, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000248 DAWBSB010000000
VERSION     DAWBSB010000248.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3214)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4915_length_3214_cov_2.253878"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2594..2636
                     /estimated_length=43
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          650 a          914 c          873 g          734 t
ORIGIN      
        1 cttcggcttt ctgttttttg tcattgtccc ggtggcaaca tccctgtatt acgcattctg
       61 caattacaac attctaaagc ccgcccgctg ggtgggtctc aaaaacttca tcgacatttt
      121 ccaggacccc acattttaca aggcactggg cgtcacgctg aaattcgcat tcatctctgt
      181 gccgctcaaa ctggtgtttg cgctgatcgt ggcgctgatt ctgctgaaag gcaccaagct
      241 gacaccgttt taccgtgccg cgtattatct gccctctatc ctgggctcct cggtggccgt
      301 tgccatcctg tggaagcgca tgttcgcggt ggacggcctt gtcaacaagc tcctcggcat
      361 ccagttcgca tggctgggca gtaccacagc cgccctgtgg gtcatcattc tgctggcagt
      421 ctggcagttc ggttcgtcca tgctcatttt tctgtccgct atgaaacaga ttcccccgga
      481 actttacgaa gcggcgcgtg tggacggcgc cggcggcatt tactccttct tccgcatcac
      541 gctgccgctg cttacgccca cgattttctt taacctcgtc atgcagctca tcaacggctt
      601 tatggtgttt gcccaggggc agatcatcac ggacgggaaa cccatgaatt ccacgttgtt
      661 ttacgttttg tacatgtacc agcagtcttt tgagtacagc aaggtcggct atgcggccgc
      721 catgggctgg atcctgctcg tgctggtagc gctgttcaca ggcctcctgt tcaagacgaa
      781 aaaatactgg gtctatgaat aagaaggggg aataacaaca tgcgcgcaaa aaaaaggatt
      841 tcccgcattg cctaccacac cgtagtgctt gcggtcggtt ttgtgatgat ctatcccctt
      901 ttgtggatgg tgatgagctc ctttaaagaa agcagcacga tcttccacac tgcgggacag
      961 ctgctgccta cgcagcccac gctggaaaac tacgccaacg gctggaaagg cttttctcac
     1021 acgacctttg ccaccttctt caaaaacaca tttttcgtgg ccgccatggg aacggtcggc
     1081 tccatcgtgt cttcacttct ggtggcctac gggctttcgc gcctgcgctt tcggctgcgg
     1141 ggcctgctgt ttgcgctggt gcttctcacc atgatgctgc cggggcagat cctgatgatc
     1201 ccgcaatacc tgtggtacaa tcaattggga tggatcaata catacagccc gctcattgct
     1261 gaattttact ttgctacaca gggctttttc gtctatctgc tgatgaactt catggatggt
     1321 atccccaagg atttggacga agcggcgaaa atcgacggct gctcgtatta ttccatcttc
     1381 tcgcgcatca tcctgccgct ctccactcca gccatcgcaa ccgttgcgat tttctcgttc
     1441 atgaaccgct ggaacgatta tatggggccg ctgctgtatt tgaagcgcac agaaaaatac
     1501 accatcagcc tggcgctgaa gctcttctgt gaccaaacct caagttcgga ttatggcgcg
     1561 ctgtttgcaa tgtccacact ctcgctggtt cccatcttcc tgattttcgc ttttatgcag
     1621 aaatatctca ccgaaggcgt ggccacctcg ggcatcaagg gataacctcc cggaaaagga
     1681 gcgtaccggt atatggatat ggcaaggatg caggcggtgg ccgataagac acgcgccttt
     1741 tatgaaacaa acaccgcggg ctgtgcactg ctgaaggtaa aggaaatcgc cacccagccc
     1801 cagcccgcgc tgtgccttac agactataac tttcccaagg acatgcgccg ttatctggat
     1861 gatcgcgccg cgcggctgct gcatgcgatg gcgctgcggg acggcctggc agacgatacc
     1921 ctgccggctc tcgcgccgtg gtacggcatt gcggagcaca gcgccttcct gggcggaaag
     1981 gtcacttttg gagaggacac cagctggcag gacccggtgc tggaagacgc atgcgggctt
     2041 tccgccctgg ctatggatga agccaaccag tcgctgcgcc ttgttgtgga cggcatcgca
     2101 tatctgcgcg aacggtatga aggacagttt ttcccgatga cgcgcggtgt ttcgggcgcc
     2161 ctggaaatgg caaacacgct gcggggcagc gatttctttt atgactttta tgaagacccc
     2221 gacgcgctga aagagcttct gaagtattgc gcgcacgcgc ttgtatggta ttacgacaag
     2281 cagcttgaag ccgcgggaca ggtattcggc ggcaccatca ccggctttgg agaatggctg
     2341 cccggcaggg ccgtgggaca gctctccgag gacaccacga ccatgatttc ccgggcgcaa
     2401 ttcgaggaat tcggccgccc gctcacacag gagatatgca cgcattatga ctctgccttc
     2461 atgcacacgc atgcgctcag cgaacattca cttccggcca ttgcgtctat cccgggcatc
     2521 cgtgccatgg agctttcgtc cgaccccaac accgaccggg ccgtcgaggt ctataaacgc
     2581 aaccgcgagg cgcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnggcc
     2641 tgttcccgtg ctgcggctga cgcgcgggga gatcgagagc aatttcgagc tgctgaagag
     2701 ccagaaaacc atcgtatggt acgacgcgtc ctgcatggag gatgcacagg atatggcggc
     2761 actgttcgcg cgtgaattcc ctgtgcgctg acaggacggc agtgccgtac aaaaggagga
     2821 aaaggctgca atgatcggaa tattccaggg aagcatgacc gccaaggagc gtgtgctgcg
     2881 cacgttcaac catgaaaaag cagaccgcgt gcccgtcaat tatcttgcca accccgccat
     2941 tcacaagcgt gttgcggcgg ctttgggcgt accggcggac aaagatgctg tggatgacgc
     3001 gctgggcgtg gacttcaagg aattgatgcc gcgctacacg ggccgcccgc tgttttctcc
     3061 acgggaggga cggcgtgtcc atcccctcac cggcgctgta cagcgctggg tgccaaacca
     3121 ggacggcggt tattgggacc actgcgattt cccgctggcg gaagccgacg acgagaccat
     3181 tgcaaactgg cccgtgccgg acccggatga tttc
//
LOCUS       DAWBSB010000249         3200 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_4939_length_3200_cov_1.901749, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000249 DAWBSB010000000
VERSION     DAWBSB010000249.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3200)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4939_length_3200_cov_1.901749"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1206..1220
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          707 a          792 c         1003 g          683 t
ORIGIN      
        1 ccacaatccc gtactgttca tcgtcatcaa tggcgtctgc tatctgggtg catccggtat
       61 gcaggtgctc atctgggcca tggtgaacga ttccatcgac tatcatgagc ttcagaccgg
      121 tgagcgtaac gaaggcattg tatactctac ttattcattt ttccgcaagc tggccagcgc
      181 tgtttccgga agtctttcca gttttgtgct gggattcatt ggctacaacg tggctgaggg
      241 agcggtgcag acggctgaag taagcaacgc catctggaag tcctacacag gaatttatgt
      301 gctgggttat ggtgtcgcgg ttgcaatttt gttctttgtc tatccgctga caaaggcaaa
      361 aacgaccgaa atgctggagc agctgaaagc ccgccgcgca gaaaaagcgt cggccgtgga
      421 aacagacggg gagtaatgcc catgaaaaca ttgcaggtat ccatgaagaa ccgggcgggc
      481 tgtacgctgc gtggcattgt gacgctgcct gagacgaacg agccggtacc ttttgtgctg
      541 aatctgcacg gctttgcagg ttcggcgtcc gggtacaagt atctgcacac gcatatggca
      601 cgcatgctgg cagaccacgg agttggctgc gcacggttcg atttctatgg ctgcggagag
      661 agtgacggcg aattttcgga catgacgttc gatggtctgc acagcgacgc ggatgatttg
      721 tacgcctggg tgctcgcaca gccttatgtg gattcaaggc gtgtattcct ttccgggcag
      781 agcatggggg ggtatgtggc cgccagcatc gcgccgcgtc tgcggccgca cgggttgatc
      841 ctgatgtgcc cgggcgcggg catgtggtat ggctgtgccc agcgggccga tatggtgacg
      901 cagagcggaa aagattatgc tgacctggag gggcttgttt acaaaatggc cttcaactac
      961 agcatggcgg cgcatccgga cccatttgaa gaggcaaaag gctacaacgg tcctgttatc
     1021 gtcatccggg cagatgatga taaacttgtg gatgacgaaa gctgtaaacg ttatgcaggg
     1081 ctgtaccagg cggctgagtt tgtacatgtt tcgggcggag gccacaattt caccaccatt
     1141 ccgtcccgca cagcctgcga acaggcaatt tggaagttca ttcaagacaa tttatgaaaa
     1201 acaccnnnnn nnnnnnnnnn gccgatagca gtgggcggaa taacgttgaa gaaccgtatc
     1261 atgtttccgc cgctcaccac gggctatgag gaacgggacg gcagcattgg ggaacagagc
     1321 cgcgcgttct atacccgtct ggcacagggc ggtgtgggat acatcgtgct gggcgacgtt
     1381 gcgcccattc gcagtttttc tcccacgcca aagttgtttg atgacagcca gattgaaagt
     1441 ttccgtttgc ttgcagaccg tgtgcatgcc tatggcgcaa agctgggcgt ccagattttt
     1501 tatccggaat acgattgcga cgcggtcaac gcgcttttcg cccaagggaa aatgaaagag
     1561 gtgcgtgccc ggctgcacca tgatatgcag ttttttactg acgaggtgag cgaggagacg
     1621 ctgctttcca tcatcgacaa aatatgtgca tgtgcgcagc gcgcgcagaa ggcgggcgtg
     1681 gacgtggtcc aggtacatgg cgaccgtttg gtgggatgtc tgtgcagcac acgcatgaac
     1741 catcgtaccg ataaatttgg cggcagtttg gaaaaccgta cccgttttgc gatcatgttg
     1801 gtgcgggcgc tcaaacgggc ggtgccggag atggtcattg actacaaatt tgccgtcgtg
     1861 acgccggaac ccggccaagg cggcgtcgat gcggcagacg ctccgaaatt tgcccgttgg
     1921 ctggaggaag ccggcgtgga tatgcttcat gtggcgcagg ccaatcatac aggcaatatg
     1981 gcggatacca tcccgcctat gggcgtacag ccctacggct tttttgcgga cattgccggg
     2041 caggtgaagg aggctgtgtc tattccggtc agcacggtag ggcgcattat cgaccccgct
     2101 atggcagagc ggatcgtaga aagcgaaaag gcggacatcg tcggtttggg ccgcccgctc
     2161 ctgtgcgacc cggattggcc gaacaaagcg cagtcgggct gtgcgtgcga tatccgacgc
     2221 tgcatttcgt gcaacaaggg atgtacggat aacatccaga accgtgcatt cctggcatgc
     2281 gtgctcaatg cggaaaacgg ttacgagaga actcgttcta tcacgcctgc tgcaaagaaa
     2341 agaaatgttg tggttgtagg cggcgggcca gctggtttgg aggccgcgcg cgtggccgct
     2401 ctgcgcgggc acagcgtcac gctgttcgaa cgcgcaccag agttgggcgg ccagctaaac
     2461 attgccagcg taccgccccg caaggaggaa atgcgccgcg ccgtattgga cttgacgcat
     2521 gctgtatgca aagcgggagt cacactgcgc cttggggctg aagtgtctgc ggaggacatt
     2581 ttggacacaa agccggacgc tgtgattgta gctgtggggg cgtccagctt tgcacctccc
     2641 atcccgggca agaacggccc gaatgtggcc gatgcttggg ccgtgttggc aggtgaacaa
     2701 caggtctatg gacgcgtggc cgtcattgga ggcggtatgg tgggatgcga gaccgccgaa
     2761 tatctggccg gacacggttg taaggtctcc attgtggaga tgatggataa gatcgccgcg
     2821 ggtgagagca ctacaatcct tcccacgctc atggaaaact acaagacgct gggcgtagag
     2881 cagtatccgg gccatacggt gaaagcaatc acaatggata cacttgtctg tgaagacaaa
     2941 gaaggcgggg aagtgctcat cccttgcgac tatgtggtaa tggccgtggg ggcgcgtcct
     3001 gctgcattcg atgcatcggc gctggaaaac gcaggcatac ccgtagtgcg ggtgggcgac
     3061 tgtgcggata aggcggcgga catttccaat gccatcaaaa ccgcatacga tgcagcctgt
     3121 gcgctgtaag agataaggaa aaggcaaggc tttccagtga acggctccag taaaagcgga
     3181 caatagacaa aaggccccct
//
LOCUS       DAWBSB010000250         3186 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_4968_length_3186_cov_2.792079, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000250 DAWBSB010000000
VERSION     DAWBSB010000250.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4968_length_3186_cov_2.792079"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2200..2209
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          677 a          935 c          953 g          611 t
ORIGIN      
        1 cgcggcttta acaaaatttc gtccgctgaa attttgcatg gttcaatgtt ctcaaagccc
       61 atgcgcaaaa gcgcatcagc gtacgaagta cgcgcagccg cgcaagcggc ggacttgaaa
      121 ttattatacc acatccgtcc ggtatgaatg ttacaaaact gtggcggccg ccggcccgct
      181 ctggcgccgt tttatatccc ggcagagggc tgcgcagctt cctgcgtatg cagagcgttc
      241 agcgcggcgc ctgcccgcgc cgccacccag agctggggcg caagcgcgcg ggcctgctca
      301 tacaggggaa cggcgccggc tgcatcgttt tcctccagaa gcagtgcgca ggccagataa
      361 tagctcgcct gcgcccggag gtagctgttg ggagcaagcc ccagctcggt gcggaaatag
      421 gcggtgtggc cgcccggctc gccccgctgc acggccatca tgtgctccag gtccagcaca
      481 gccttgctga aatgcccgaa cagcgcgttg cccgccggca gcgtgcgcac ttcgtcacgc
      541 agatatgcga gctggctgcg ggccgcggcg taatcttcca ggccgatggc gatgctggcc
      601 tggcgcgtat aaacgctcag gcggagctgc tgcaggcagc ttttgctgcg gcggggcacg
      661 cggtccatcc ccatgcggga cagcgcctcc agcgcctcgg ggccgcgccc cagcgcctgc
      721 agggcaaagc tttcattcaa tgccaggttg ggccgcgtgc tgcggttctg ccagcgttgg
      781 cacccgcgta tctcggccag aaacgccgcg gcgtcgcagc tcgtaagaaa gcgctggtac
      841 gccgcattga agcggcgcgt gcccagcacg ctgcaaatca ccgccagcag catcacaccc
      901 gcgagcgtaa gcaccagaag cacgccgaaa gcggcccagt gcaaaaaaat accggcgata
      961 taaagtgaaa gcatcagcag acagccgatg gaataggcgg ccgtgtagcg tccgtaataa
     1021 cccatataca aaatcaaacc tttctgcatg cgtgcggatt ttgtcctttt gtgttattat
     1081 agtatagcac accgcgcgcc ccgggcggcg gtgtggtttt gtaaaaaata gccgcaggct
     1141 gtttttcgcc tttccggccg gtctctgggc gggaacgccg ggatagtttt gctgtgcaac
     1201 aagaaaatgg ccgtgggccg tttttacggg cacaggcggg aaagcggcgg tccgggtgtg
     1261 cgcacgcagg gcgcggcgcg ggaacaggcg gacgcggccg aaagggagag agcagatgaa
     1321 tgcagatttt tattccgaga gctttaccgt aaaggcatat gaatgcgatg ccgaggcccg
     1381 tatgacgccg ggggccatcc tgcggcgcgc ccaacagata tccaccgacc agtgcgacct
     1441 gctgggcctg acgaatgagg tgtacgcgcg cacgcacacc gcgtttttgc tggcgaagct
     1501 ggccgtggag ttttatgcgc ccgtgcgcgc ggggcagtgc gtgacgctgg caaccaggcc
     1561 cagcgcgccc cagcgcgcgg tgtatgggcg ctacaccaca ctgtgcgcgc aggacggcac
     1621 ggtgctgagc gcagtggaca gccgctggat cttggtggat acacggacga agcgcatcct
     1681 gcgccgtccg ccggaggaca tgcccatgcc gttcgtccag ccgcccgtat gcgagctgga
     1741 tgtatcgctg gtaaaagggg aggcgcagcc tgtggcggag gaaacggcct tttatacccg
     1801 ctgcgaccag aaccgccata tgaacaacac ctattatgcg gatatcgtct gcgaccatgt
     1861 gcctctggag cgcattgccg cgcacgcgcc cgcgcggctc gccgtcgtct atcacagtga
     1921 ggtgcccatg ggcgcttcct ttaccctgct gcgcgcgcag acaggggaga acggctggta
     1981 ttttgtagga acggacgggg aaaagaaaca ctttgaggca aacctgacgc tgaccgagtg
     2041 agcgggcggc aggccttccc ggcggcggaa cgctttttcc gcagctgctt tgcggagcgg
     2101 ctgtgccttt tgcagcgggg aacgcccttt ttggcaaatt ttcgcctaaa aacataccaa
     2161 atgtgtaaga attgtaaaag ttaaaaaata gttacaattn nnnnnnnnnc ctattgacag
     2221 gtgatgtcaa aagggttaca atttagttgc aagaaagtta caaggctctt gcaagtgcac
     2281 tttttcatcg ggtgcaggcc ctctcctcct atcgttgttt tggctagaaa gcagcggtcc
     2341 tgggtgctgc accaaccttt tcaaactcca tttcacaagg caaaacggcc ccgtatgctg
     2401 catacggggc cgttttgtac catttgccca caattctccc ggaaatagag gttattttct
     2461 gtgcgcagga cgcgcacttt tgtgaaaaaa cctttgcggc acgcggccct gtgtgctatt
     2521 ataatgggta aacaattaaa aagaaaagga gatcactatg gccacttact tcacggaacc
     2581 ctcccgcacg tttggcgaat atctgctggt gcccggctat tcgtcgtccg agtgcatccc
     2641 ctccgccgtc agcctgaaga ccccgctggt gaaataccgc aaggggcagg aggattgccc
     2701 gctttccatg aacatcccca tggtatcggc catcatgcag tctgtgtcca acgacaccat
     2761 ggccatcgcg ctcgccaagg agggcggcgt ttcgttcatc tacggctcgc agtccgtgga
     2821 ggacgaggcg gcgatggtcg cgcgtgtcaa aagctacaaa aagggcttca tcgtcagcga
     2881 ttccaacgtg cgccctgacg ccacgctggc cgatatcctg gccctgaagg aaaaaacggg
     2941 ccactccacc gtggccgtaa ccgacgccgg cacggccaac ggaaagctgc tgggcatcgt
     3001 ggcaagccgg gattaccgca tctcccgcat gagtacggat ctgcccgtga gcgagtttat
     3061 gacgccgttt gaaaagcttg tcgtggccga cgaggacacc accctcaagg aagcgaacga
     3121 catcatctgg gacaataaaa tcaactcact gcccctcacc gacaagaacc agcacctgaa
     3181 atgcat
//
LOCUS       DAWBSB010000251         3168 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_4994_length_3168_cov_3.548346, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000251 DAWBSB010000000
VERSION     DAWBSB010000251.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4994_length_3168_cov_3.548346"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          695 a          825 c          997 g          651 t
ORIGIN      
        1 tgtgacagcg cctatatgct acaatatttt agacataaag atattattcc ccaaaggaat
       61 ttgcttgtgg tatggatatt tatttaacac tgtacaggaa aaaacggcga gccgccgcaa
      121 tcatcaaaag gagtgtagag aatgcaggaa atgatcagcc agaaggtgcg gacgctttcg
      181 ccggagatcg acccgctgcg catgggtatg gggcagacgg aggccgacct ggaaaaaccg
      241 caggtaatgg tggagagcac atttggcgac agccatcccg gcagcgcgca cctgctgtgt
      301 ttggtggaag ccgccgctgc gggagtacgc agcgcgggcg ggaaccctgc gcgctacttt
      361 gctacagata tatgcgacgg catggcgcag ggacacgacg gcatcaatta ctcgctcgct
      421 tcgagggaga tcattgcgga tgccatcgag atccacggta tggccacgcc ttttgacgca
      481 acggtataca ttgcaagctg tgataagggc ctgcccgcga atctgatggc ctccggccgg
      541 ctgaacacgc cgggcatatt tgtgcccggc ggtgtgatgg atgcggggcc ggatctgctg
      601 acgctggagc agatcggtac atacagcgcc caatatgagc gcggcgagat caccgcggag
      661 cagttccggt tttataagac ccatgcctgc ccgtcctgcg gagcatgcag ctttatgggc
      721 acggcgtgca cgatgcagat gatgaccgag gccttggggc tttcgcttcc gggcagcgcg
      781 cttgttccgg ccgcaagccc gctgctggca gactatgcgc ggcgcgcggg cgaacaagcg
      841 gtagcgctgg cgcgcgcggg catccgcacg ggagatatcg tgacaaaaga ggcatttgaa
      901 aacgccgtcc tcatccatgc cgccatttcc ggctccacca atgcgatgct gcacctgccg
      961 gccatcgcac acgagtacgg catagagctg gactgcgata tgtttgaccg catgcaccgc
     1021 ggggcgcggt ggctgctgga cgttcgcccg gcgggcaggt ggcccgccgc ctacgtgtgg
     1081 tatgcaggcg gcgtgccgcg cgttatggag cgcctgcgcg atctgctgca tctggatgta
     1141 ctgacggtca cgggcagaac actgggcgaa aacctggacg aactgcaaaa gaacggattt
     1201 tatgaaagct gtgcatcgta tctgcagggt acgggcgttg caccggagga cgtcattcgc
     1261 ccgctggaaa aaccgttggg cacagatggc gccatcgctg ttctgcgggg caaccttgcg
     1321 ccgggaggcg ccgtggtcaa acacacagcc gtgccggagg agatgttcgg cgtaacgctg
     1381 cgcgcacggc cgtttgactg tgaggaggac gccatccatg ctatcctcac acacgccgtc
     1441 catcccggtg aggcagtgtt catccgctac gagggcccca aaggcagcgg tatgccggag
     1501 atgttctata ccaccgaggc gatctcgtcg gacccggcgc tcgcccgaag catcgcgctc
     1561 atcacagacg gccgtttctc cggtgcctca aaggggccgg tcatcggcca cgtcagcccg
     1621 gaggctgcgg acggcgggcc gatcgcactt gtggaggagg gagacctcat ccgtatcgac
     1681 atctgcgggc gcagcctgtc catcatcgga gcaaatggcg ccgagaaaac gccggaacag
     1741 atggaggcga tcctcgctgc acgccgtgct gcgtggagtc cccgtccgcc ccgatataca
     1801 aaaggtgtgc tccggtttta cacccggcac gccgtatcgc ccatgcgcgg cggctatatg
     1861 gaatagtaca ttgccccagt gtagggcgta cctctccgtt tcgtatgagg gaaagagaaa
     1921 gctgtgataa tggatgaaag agacatatca gttatttgta ggaacataca gcaggccgat
     1981 ccggtttggt acaggggaaa tcctggaggg caggggaaaa ggaattcata ggtatacctt
     2041 tgatgccaaa acgggcacgt tgtatgaaag taccgctccg gcgccggccg aaaacccgtc
     2101 ttatctggcg ttatcttttg acaaacagta tttgtatgca gtaaatgaat tgaaagaata
     2161 caaaaataaa gcgggcgggg ccgtgagcgt ttatcggata gagtcggacg gtggattgcg
     2221 ttttctgaac cagaggccca ccggtggtgc cgacccatgt tatgttgggc ttgacaggga
     2281 aaggcattgt cttacagtag ctaattttac gggaggaagc gtatgcagct accctctgtg
     2341 tgcggatggc tcactgggaa aaaagggcgt gattatccgg cattacgggc atggagcaga
     2401 ccctgtgagg caaagcgccc cgcaccctca tgcggcggtt tgggctccgg atggaaaata
     2461 tgttattgta gcagaccttg ggacggacga tctgacggtt tatcgggtgg accgggaaaa
     2521 ccgcgtcctt tgtgcggatg cggttcatag cttttttgtg ggcagcagga tgggtccgcg
     2581 tgcctgtgtg ttcgaccaaa ggggcgaacg gtgctatgtg ctctgtgaga tatcctctgc
     2641 agttatgacc ttttcttaca tggacggaag actggaattt ttacaagccg ttccttccgt
     2701 ggcagagccc ggcggcgtgt ccaacagcgg agctgacctt catctggcgc cggacggccg
     2761 cttcttgtat gtgtccaatc gcggacagga cagcattacc gtcttttcgg tacaggcaga
     2821 cgggacactg caattggtgc aggccgtggg ttgcggcggc aggacgccgc ggaattttgc
     2881 actggacccc acaggagggt gggtgcttgt gggaaaccag gacagcgaca gtattgccgt
     2941 gttcaaacgg gacgtccaaa gcggaaggct ggcgctggaa aacaaagctt ttgcgccgac
     3001 gcctgtctcc cttttgttcc gggcataggc cgggacgttc tctatacagc aaaaacaagg
     3061 ctgaaagcaa tgcacgcagc attgctttca gccttgtttt tatatgcgat aacagcggaa
     3121 aatattttgc gttgggtaaa attatatcca taacaagaac tatatgat
//
LOCUS       DAWBSB010000252         3121 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_5065_length_3121_cov_2.736791, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000252 DAWBSB010000000
VERSION     DAWBSB010000252.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5065_length_3121_cov_2.736791"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1191..1200
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1985..1994
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2716..2725
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          655 a          942 c          948 g          546 t
ORIGIN      
        1 acccgccggg cttcacagaa ggcccggcgg ctgtttaggg ggagtgcacg gtccagaata
       61 cgtcggtgca gtggcgcggc ctcggccggg caaacggtgc gtcaaccgtt ctcccagctt
      121 gaagtatttg cggaaatgcc ggcgagcacc tgttccagaa gcgggcggga acaggccgca
      181 agcactgcgc tgcccgtgtc ctgcggcaga ggggctccgt ccagcgcaca gatacagcct
      241 ccggcctcag ccagaatgat gcgcgccgcg ttgtggtccc atggacacga acgcagcgcc
      301 acaaacagct ctgcttcacc cgcggccacg gcgcacagct ccagtgccac acaccccatg
      361 ctgcgcaccc cgcggacagc tccggcaagc tctacaagcc cagcgcgccg ggggtgctcc
      421 cgcaaaaaag tgtgcagaac gcccggtgtc gtgagcagtg cgtcccgcag cacgccgcag
      481 tgcgaaacat gaatgggcgt ttcatcgcgc agtgcgccgc cgccgtgccg ggcggagtac
      541 agtgcgccgc gttcaacgtc cagcacaagg ccgaaggcag gcacaccgtc ctggtagcag
      601 cccacagaca ccgcatagtt gcggtgctgg ctgacaaaat ttgttgtacc gtcgatgggg
      661 tccagatacc acacgcagcc cgtaccgccc gggggcgtgg cgggatattc ctcgcctacg
      721 atggcgtcgc ccggaaaggc ggacaggatg ccgctgcgca ggaactgttc cgtctcgcgg
      781 tcacaacgtg ttacaagatc ctgatgcccg gttttctccc aaaccgcgcc ggcctccggg
      841 cgggccagac gcaggcgcgc gcccgccgcc agcaccagcg cgcgggctgt atcaaagcgg
      901 tccatgttcc tcctcctttc cgcaacatcc tgcttttatc aaagcataac cggcatattg
      961 tgtcaagtgt gttaccatat agcctccatc cggccgttac acggcaggga aacgggactg
     1021 ttggcgcgca catactctgc cacaagctca ggcatttcca cgctgccgcg ccacaatacg
     1081 gggcatgtgc gcagtgcgtc gtagccaccg gtgcccgtgg cacggtaatt gctggtacaa
     1141 agccgcagag gcccaggccc caagggggtg ccgtcaagcc gggagagccg nnnnnnnnnn
     1201 cggcccacag gctgccgcag atcaaactga taagagatcc cagcgaagaa atcgtagttg
     1261 taatgctcca ccttgggggc gagaaaagca tcggacaccc ggggcgttcc gttttccagc
     1321 gtgaaatagg aggcgcagcg ttccagaagc gcgcgcagga tctcttccgt aatctccagc
     1381 accaccagtg tgttggagaa caagtatgcc gccgtaatgg cacgcatggt tacggacgcg
     1441 gcaaggcctg cgggttcatt gccgaggctg gtgcaggaga tgtcggcacc ggtgtgtgaa
     1501 agctggactt ggttgaacaa tgccgccaca gcgctgccgt gcagcgctgc gcgcagcttt
     1561 tcctcagggg ggatggcctg ctccagatgc cccacaggtt cgtccagcca gcgctgtgcg
     1621 gcctgctcca gaggcaggag gcggcggtaa ggctgttctc ggtgtatgcc gcccacaggc
     1681 agcaggcgtg cggaaaattc tgcaccgccg tcccgtaccg cggcttccac ctgcagatag
     1741 cgcccggcat tggcgggggg ctgcacggcg aaggttccgc cgatgcgcac gctttctacg
     1801 ggcatgtgct ggtggcccgt aagaagcagg tcaaagtcaa gctcgcgcgc gatcctgcac
     1861 gcaaggtttt ctccgctgcc ggacagcacc gcgcccgtgt gcagatcctc ttcaaagccg
     1921 ccgtgataga tacacacgca tatgtcacat atcgggcgct aacgggcaca ggcagcacgc
     1981 gcggnnnnnn nnnntttcag attctggggc tgctcccaga cgtttacata atcggtgaca
     2041 atgcctgtga tgccgatgcg caggccgttt ttcagcgtgt ggacagtctc cggaagcaaa
     2101 ggcagcgcgc cgcccagatc ttgtacattg gcgcacaggc acacgccgtc catggcgcac
     2161 agatatgcgt gcagcgcgtc atagccgaag ttgaaatcgt ggttgccaag tgtgaaataa
     2221 tcacagccca tggcattgaa ggcttcggcc acaggatgat ggggaaattc gtccgcgtgc
     2281 gccagataat actgcaccag cggcgtgccc tgcaaagagt ctccgccgtc cagcaccagc
     2341 gtgtttccgt tcttgtcgat ttgtgcggcc aggttcagca ggccggagct ttcgccgcat
     2401 ccggcggcgt agttgacagg gaagatatgt ccgtgcgtat cggacgtata aataatttta
     2461 aaagtacgtt ccatgttcag cctctcgcca attttatgcg gatttttgtt gagagccact
     2521 ccacggccag caccaaaacg atcagcccgg caagaatggc accggcttcg ctccaacggt
     2581 agctgttcat tgcaaagata agcggcgcgc cgatgccgcc agctcccaca aggcccagaa
     2641 cagtagcgtc gcgcaggttg atgtcaaaac ggtagatggc ggtggaagcg aagttgggca
     2701 tcagctgagg caggannnnn nnnnnttcca gcacgcgtac atccaggtct tctatggcct
     2761 caatatacat tttgctcacc atgcccacag agcacagcga catcgtcagc aggcccgcga
     2821 aagcgcccgg tcccgtgaca cggatgaaca tcagcccata gatgaacgcg ggcaccgtgc
     2881 gcacggccat aatgatgatg cggccgataa aagcaatggg cttcggcgtc aggttggacg
     2941 cggagagaaa cgccagcgga atggagatga cggcgcccac aacagtgccg agaaatgcaa
     3001 tgcatacggt ctcaagaagc agatagggaa caccctgcac cgtcagattg aacagcaggc
     3061 ccgtgtccgg atgaaagatg ccggaaagga tgttcagagc gattttgccg ccgttctgcg
     3121 t
//
LOCUS       DAWBSB010000253         3114 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_5082_length_3114_cov_2.859758, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000253 DAWBSB010000000
VERSION     DAWBSB010000253.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3114)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5082_length_3114_cov_2.859758"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1911..1920
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          624 a          857 c          906 g          717 t
ORIGIN      
        1 tttccgcacg gcctcccgtt ttcaagggcc ggctcaactg gtaaaagggg gaattttttt
       61 atggcgcacc gcaatccggg caaggcggat ggacgcagcc acgcactgcg ccgccacgac
      121 aacatcgtcg gttatctttt catgctgccg gcactgctgt ttttctgctg ctttatcatc
      181 attcccatgc tcacaggctt tgttacgagc ctgtttgatt acaccatgaa gaccccggtt
      241 tccatggaga ctttcgtggg cctgcgcaac tacgcagagc tattcactac gcccacattt
      301 taccgtgcgt tgctcaatac gctgctcatc gttgtagttg cggttcccac cgtcaccgcg
      361 ttttcgctgt gggtggcttc cgccatcacg ccgatgcacg aggcgccgcg ttcgtttttt
      421 cgctgtgtgt tctatctgcc cgcggtcacg ggcaccgtgg cggtcgtggt cgtgtggaaa
      481 tggatgttcg atgcttataa cggtattttt aactgggccc tcaaatctct ggggatcatc
      541 gatcagaata tcatgtggct cggcgacgcg cgttttgcca tctggtgcat tatcctcatc
      601 ctgttcacca cttcggtggg gcagcccatt gtgctgtacg tagcggcatt gggcaacgtg
      661 gaccgatccc tggtggaagc cgccgaggta gacggcgcca ccaagctgca gaccttctgg
      721 aagatcaaat ggccctccgt catgcctacg acactgtatg tggccatcat taccaccatc
      781 aacagcttcc agtgtttcgc actcatccaa ctgctcactt ccggcggacc gcagcattcc
      841 accgacacgg tgatgtatta cttatatacc aatgctttca cgctgtaccg ttatgggtat
      901 gcaaacgccg taggtgtggt actggccatt atcattgcgc tgttcagtgt tctgcagttc
      961 aaaacgatga acaccggcgt ggaatattaa ggagggggaa gcgcaatgga aaaaaataaa
     1021 aaagcctata cagtttttgc gtgggcggcg ctgtgcctgc tggctttcac cttcatcttc
     1081 ccgctgtatt ggatcgtgac cggttcgttt aagctgcaaa cggcgacggt tcagctgccg
     1141 cctcaatggt tccctgcgga gcccacggcg gcaaactatg tcaagctgtt taaaaacccg
     1201 gcgggccttt ggctgtggaa cagtgtgttc caggccgcca tggccacgct gctgacatgc
     1261 tttaccgctt cgctggcggg ctatgcattg gcgaaaaagc gatttacggg cgcaaaattc
     1321 atttttgcca ttttcgtatg cgctatggca ctgccaaagc aggtggtgat ggtgcccctg
     1381 gtgcgcatta tggcggccat cgggctgtac aatacctcat gggccgtcat ctttcccacg
     1441 gtcggctggc cgttcggcgt gtttttgatg aaacagtttt ccgagactct gccctccgag
     1501 cttctggagg ccgcgagaat cgacggcgcg ggcgaactgc gcaccttctg ggatatcgtg
     1561 ctgcccatcg tcaagcccgg cgtaggggcg cttgccatct tcaccttcgt caatacctgg
     1621 aacgattatt ttatgcagct catcatgctc aaaagcagcg atgccatcac ggtctcactg
     1681 ggcattgccc ggctgcaatc ggagttcgcg acgaactacg ggctgatgat ggcgggtgcc
     1741 acattgggcg cggtacccat cgttgcagta ttcatctgct tccagaagta ttttacgcag
     1801 ggcatcacca tgggcgccgt caagggatga cggccgcctg catatatccg gcggcggcga
     1861 gctgtttgcc ctgtgccgcc ccattgagag aggaacgatt tgtatgagag nnnnnnnnnn
     1921 gtatgagaga gcctgaaaaa tattgcggcg tcatccccgc gttctatgcc tgttatgacg
     1981 acgcgggcga agtgagcccc gaacgggtgc gcgcgcttgc agagcatctg attcgaaaag
     2041 gcgtcaaagg cgtttatgtg ggcggctcat ccggcgaatg tatttaccaa agcgtagacg
     2101 accgcaagcg cacgttggaa aatgtgatgg cggtagccaa agggcgcatc acggtcatcg
     2161 cacacgtggc gtgcaacaac acgcgggaca gcgtggaact ggcgcgccat gccgagagcc
     2221 tgggagtaga cgccatcgca gccattccgc ccatctactt tcacttgccg gaatacgcca
     2281 tcgccgatta ctggaacgcg atgagcaacg ccgcacccaa caccgacttt gtgatttata
     2341 atatcccgca gcttgcgggt gtggcgctga cgatgcccct gtttgaggag atgcgcaaaa
     2401 atccccgtgt cgcggctgta aaaaattctt ccatgccggt gcaggatatc cagatgttca
     2461 aggctgcggg cggcgagggc ttcgtggtgt tcaacggtcc cgatgaacag tttgtgggcg
     2521 ggcgcacgat gggtgcggac ggcggtatcg gcggcaccta tgcagtgatg ccggagctgt
     2581 ttttgaaggc cgatgcgctt ttgcgaacag gcgaggttga aaaagcctgt gccgtccagt
     2641 atgatattga tgcgatcatc tacgccatgt gcgcctgccg cggtaacctg tatgcggtaa
     2701 tgaaggaaat tcttcgcctg cgcgagggcc tgattctggg aggcgtgcgc gcgccgctgc
     2761 cgggcctcgg gcctgaagat atgccccagg tagaaaaatg tgcggcgatg atagacgcgg
     2821 ccattgagcg cgctgccgct ttataacgct cccccttcca gtcccggacg cagggcacgg
     2881 gcggcggccc tgccctgcac cggggctttt tgcattggga acgccctgca taccagggtt
     2941 catgaaccgc acacgaaaaa gccggtgccc ggtgggctgc ggcgcttatt gagcggagaa
     3001 gccgcaatgc ggacttacag caacggcgca tctgcgccgg aacggcaagg agtgaacaaa
     3061 tgaaaatctc atgtatcgac atcggaggga catacatcaa gtcaggcgtg ctgg
//
LOCUS       DAWBSB010000254         3055 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_5194_length_3055_cov_2.184000, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000254 DAWBSB010000000
VERSION     DAWBSB010000254.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5194_length_3055_cov_2.184000"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2023..2032
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          659 a          900 c          776 g          710 t
ORIGIN      
        1 ccattttgtc gaggaacacg ttgatcaggc cgttgccctg gttgaagaac aggttcagga
       61 tgcctacaag aaccaccaca gaaataaaat gcggcgcgta agaaatggtc tgcacggctt
      121 ttttcagctt tgggcttttc acctcgttga ataccagcgc cagcaaaatc gggaacggga
      181 acgccactat aaggttatac aggctcaacc ctaccgtatt tcgcagcagc ctccagaaat
      241 aatatgaatc aaagaaacgg ttcaggttct ccattcccgt ccacgtgctg ccccagatgc
      301 ccagccgggg cgagtagtac ttgaaggcga tctgcacgcc gtacatcggc agataacaaa
      361 aaataatgaa atagacgatc gcagggatca aaaacaagta aaggctcttg ctttggttga
      421 gctttgtgag cccccgcctg cgccgctgcc tgccgcgcgc ccgcttttcg gcgcgtgtcc
      481 gtgccggtgc ggccgtccgg ccgccctgca gcattttaac catttttttc accgtccgtc
      541 ctttggattt gtgttcatag tacaacccaa aacacgattt tgccatgtgc aaaaattgac
      601 atccgccgca atatttgccc agatacaccc ttaattcttg tttttattgt taaatattcc
      661 tataatattt ttacaacaga agaaaaaaca gcaaaattct acaattggag gtgatgagta
      721 tgaaagaaaa gctgcccggg ctctttcgtc cgctgatgaa attcaatgta accgctgtaa
      781 tggtcatcat tctgatgttc tctgttttca atgttttttc cattgtccgc acacagcagc
      841 ggcaggccag cctgcagacg atggccctga gccaggccag ccagcaaatc ggggatttca
      901 tcgccagtgc cgaacagttg ggcatcgtcg tccagaatga ttcccagctc atggcttata
      961 acctttataa taacagagac ctgccccaca atatcgtctg ggagctggaa atgctggcgc
     1021 cctccgtcaa ctctctctcc tccctcagca tcgtttacgt agattcgctg tacccgcaga
     1081 tcaacggcgt catctattcc agcgccgggc ggttcagcta cgccgattac tgccgcgacg
     1141 aactgagcgg cagcctcacg gaaaaccagc ttatggaagt ggtgtatgcc gcggatcatg
     1201 cccatttcgt gccccaactc tcaggcaata ccagaagcct tttgtatgtg atgcccatcc
     1261 cctcctcgta tgtgttctct cccggcagac tgctgctgcg gctggactat acctccttta
     1321 cacgcgggct gcgcgccaca gcggccggaa acagcgacat ttatgtattc gacagcgaag
     1381 aaaacctgct tttgtacttt ggccccactt acggcaacga tatcaccgcc gcccgtgctg
     1441 ccgcagtcgg gcctgaaagc ctttccggca aggccgtatt tcgccacgag ctggagaatt
     1501 acggtatcca ggtggtgcag gccctggatt tctggcaata ttatcagcct acgctcacag
     1561 ctgttctcag ccttgtgctg ctggcagctt tcgtcacggt gtcgggcctc cgcctttctt
     1621 atgtgctggc tcaaaatacc gcccgcccgt tttccgtatt ggtacagcgc gcctcccagc
     1681 tgggcggcgt gacgcccgcg gaggaaccgg atgagatcgc ccggctctct catatttttg
     1741 aagacctcgt ggagcagcag acttctctga cagccgctat ggagagccac agccaactgg
     1801 aggaatccca gtacctgctg tatctgctgg gcgtgcttgg cgggcgggac ttttccgaaa
     1861 ccgtcgtaca gaaatacagt acgctgcttt cccgctggag ccgcttcggc ctgcgcgtcc
     1921 tggtgttccg ctttgacgat accattcatt ttgtggagac gtatctcccc aagcagcaat
     1981 ggagcatcaa atccgctttg cggcaggaat ttgccgcgat gtnnnnnnnn nngcggtcga
     2041 cctgcaaaac gggcagggga tcgcggcggt cgctgccttc gggccggacg cagactgcgc
     2101 ctgcattctg gacgccatcc gcgaagcgct gtgccgcagc tgcgggctgt cgctctcctg
     2161 cggcatcagc agcgcggtct atgcggccgg agatctgccc catgccttcc gggaggcgag
     2221 cgccaactgc cggtaccgcc tcttccacgc caattccacc attacgccgg aatttgtggc
     2281 cgacatccgc aaccagcgca cgcgtctctc ttatacgggc gcggacgata ttcttcaagc
     2341 agtcaaggcg tgcagctacc cggacatctc ccgcggagtg gggcgcttct ttgccgatat
     2401 cacccgggcc gccacccttc cctcctacaa actcgcctat tttgacatgc tttcacgcct
     2461 gaacaagctg attccggaat gcgccgaaaa caagcagacc atgctggtaa acatgttgga
     2521 cctgctgtat gaaaactgtt acgagagcac ggaagtgctc cagcaggacc tgtacaaatt
     2581 ctgtatcctg ctggccgata ccctgtacga gcagcgctcg gcgcagctga gcggcggcct
     2641 gctgggcacg atttatgact atgtggagga aaattatgcc tcgcccgcgc tttcgctttc
     2701 ctccattgcc gagagcctgc gtttttctcc ctcttatctc acccgttatt ttaaggaaaa
     2761 gacaggtatt tccctgatgc agtacatcga ccgtaagcgc tttgaggaga gcaagcgcct
     2821 gctggctgcc accgctttgc ccgttaaggc cattgtggag cgcgttggct acaccgacga
     2881 ggcgaatttt tcccgcaaat tcaaaaaata tgaaggcgtt acgcccactc aataccgcgc
     2941 catgcagaaa taacggcaaa agcccggcgc tttcccgttt ctgggaaagc gccgggctca
     3001 gcttgtcgaa aaagctgcgc ttttgcgcca agctacctac gagcagggcc tgcgg
//
LOCUS       DAWBSB010000255         3044 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_5219_length_3044_cov_2.170291, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000255 DAWBSB010000000
VERSION     DAWBSB010000255.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3044)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5219_length_3044_cov_2.170291"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1731..1740
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          719 a          963 c          760 g          592 t
ORIGIN      
        1 ggacgaaccc tcgtaaaatt ccgctacggt gcgcgtgctg atgagctgct gcgaacacac
       61 cacgcaaaca aggaccacaa aacccagaag gaggttcatc acagcacccg ccacggtgac
      121 aaggattcgg tttcccaccg gggcctttgt aaaggaaccc gcctcttcgc tctcctcgtc
      181 ctcaccctcc atactgacaa agccgccgat gggaaacagg cgcaagccgt attttgttcc
      241 cccgcgggtg aagctgaaca gcgtaggccc catgcccagc gcgaattcgt tcacctggat
      301 gccgctgaac ttcgcagtga ggaaatgccc cagctcatga atgaaaatca caatgccgaa
      361 tacgaatacc gacgccaaca ccgtaatgag aaacgacccc gcgctgctga aaaatgatgc
      421 cattctgtgc cctgcctttt atttcatttg ttcggccgcg ccgtgcggac cgtataacag
      481 cccggcgcgc cgccgcccat tcttttagga tacatgtgct aaaacatatg cgcgcgccgc
      541 cgcgtcgcat gcatacacgt catccagagt atacggcccg ccgaaggctt cgctgtccac
      601 aacgccctcc accagctcac cgattttcag gaacggaatg cgtccctcca gaaacagacg
      661 caccgcctct tcgtttgcac cgtttgcagc acacggtgca aggccgccct tgccgattgc
      721 ttttttacat gccgcgaggc agcggaaagt agcctcatcc gcggtgtcaa aagtcagctc
      781 gtgcagcttt gtgaagtcca gctccggcgc agggccgggc acacgctccg gccaggtaag
      841 cgcgtactgg atgggaatgc gcatatccgg cacccccaac tgtgccaaaa tggagttgtc
      901 actgaactgc accatcgaat ggataatact ctggcgctgt accaccacct gtacctgctc
      961 tgccggcagg ccgaacagcc agaccgcctc gatcagctcc aggcccttgt tcatcagcgt
     1021 ggcggaatca atggtgatct tgcttcccat actccaattg ggatggcgca gcgcatcgtc
     1081 ctttgtaaca ttctgcagct cctgccgcgt gcggccgaaa aacggcccgc cggaggccgt
     1141 caacagtatc ttttgcaggc ttttggccga gtattcgtcc tgtaagcatt ggaaaatggc
     1201 cgaatgctca ctgtccacag gcagaagctt gacgttattc tcccggacgg catccgtcac
     1261 gagggaaccg ccggtgacaa ggctctcctt gttggccagc gccacatccc gcccgctttt
     1321 gatggcggac agcgtcgcgg caagcccggc aatgcccacc accgcgttga gcacgatgtc
     1381 cgagccgtcc atagctgcca attcttcaag accttctttg cctcgcagaa ggaccggcgc
     1441 gcccggcatg ccgtgcagct cccgggcggc gcggtccgcc gccgcgtcat ccacaacagc
     1501 aatgcaatga ggatgaaact cctgtacctg tcgcagcagc agctccacat tggaatgcgc
     1561 cgccagccca aacacgctgt attcctgcgc gcggataaca tccaggctct gcgtaccgat
     1621 agaacctgtg gagccaagga ccgtgacctt ttttgtcata atatcacgcc tcgttatttc
     1681 gtaaaatagt aaaataccgc cgtcacgacc attgcgacaa acggcgcgat nnnnnnnnnn
     1741 tccataatgc ctccgtgccc gggaaagatg gtgccgtaat ccttgatgcc gcactggcgc
     1801 ttgatcacag aggcaaacag atcgcccacg atccccagca gagagccgaa cccacccagc
     1861 agcacaacga ccacataata ccgccagccc agcgtatcca gaggcacgcc gacaagctgc
     1921 ccgtgcagcg ctgtatagca tgccgtaaca acaacgccca gcagcatgct tcccatcacg
     1981 ccgcccacgg ctccctcgat ggtctttttc gggctgacga ccggcgccag cttatgcttg
     2041 ccgaacgcgc gtcccgcaaa atatgcggcg gaatccccgc cccaggcaaa gcccaaaatc
     2101 aggaaaataa aatacagcgc atcgtagccg tatgtctcgc gcggcagaag ctgcttgaga
     2161 taaatgaggg aataaaagca gaacatgatg atgcaggaaa acagcaccat gccgcccagc
     2221 tttgcataat tgatggactt tgaattgccg atgacacaca aaacgaggta caacgcaaaa
     2281 atgtaggacg ccggaagcat acagatgcga accatatgga aatcgctgaa catcaccagc
     2341 agcgtatacg gcacaaagcc ccaataaata tacgcagcct tttcaaaccg gaacgccttg
     2401 aacacctcat ggatggcgag caggcaaatg gccgcaatga cgacgttgaa cacgaaggtg
     2461 tcgaacagcg ccatcaccgc gaacagtatc accaggccga ctgcggaggt aatcactctt
     2521 gttttcatag ccatgcctcg ctttgctttc aaattccgcc gaagcggcgg ctgcgggtat
     2581 tgtattcttc aatggcccgg tccagatccg cccgggtaaa atccggccat aacacgtcca
     2641 tatccacaaa ttccgcataa gacgcctgcc acagcataaa attggagagc ctgtgctctc
     2701 cgctggggcg cagaatgaaa tccggatctt tctgcccggc ggtatacatt acgcccgata
     2761 ccgtctcaat gtcgatgtcc tcaggccgca gcaacccctg ctgtacacgg cgtgccagga
     2821 tctgcatccc ccgcacgatc tcctgccggc cgccatagtt gacagccacg ttgagtaccg
     2881 taccggtgtt gccgcgggtt ttttcctcga tatcgttcat ctgcgcctgg tatttggcgg
     2941 gcagcgccgt gcggtcgccc aaaaacacca cacggttgtt ctccttctgg taatcgtacg
     3001 ccataataag gtattcgccg aacagcttca taatgccgtt gact
//
LOCUS       DAWBSB010000256         3042 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_5223_length_3042_cov_2.869099, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000256 DAWBSB010000000
VERSION     DAWBSB010000256.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5223_length_3042_cov_2.869099"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          656 a          881 c          970 g          535 t
ORIGIN      
        1 tgtaagcagg agtgaacagt ggtgtttttg gcatagaaaa agcttgcctg accgaaacac
       61 acagccgaat ctctgtgtgt ttgccggtca ggcaagcttt ccgttgttat tgagatgggt
      121 tttttattgc ggagcaatca aaaatcagtt gctgctgctg cggcggccca gcaggcgcag
      181 gatatacagg aagatgttga tgaagtccag ataaagctgg agcgccgcga agatggacgc
      241 tttcttcgac atttcgtaat cgccttcgaa cgctgcgtgc agctgtttga tcttctgcgt
      301 atcatatgcc gtcaggccca tgaacacggc caggccgaca aagcagatga tgcggtcgaa
      361 cccggagagc ggcaggaaca tggagatgag ccagaatacc aaaaggaaga tcagcccggc
      421 cgtcaggata ggacgcaggc cggaaaggtc gcgcttggtg aaccagccgt aggccgccag
      481 cacgccgaaa tacagcgagg tcagcgcgaa cacggtgagc aggatctgaa cgtcgtaagc
      541 gaaaaagacc gcggaaaacg tgatgccgtt gagcaccgcg taggcgaaga acaggccgcg
      601 ggccgcgccg atgctcagct tatgcagacg ggcggaaagc accatcacca cgacgatctc
      661 ggcaatggca agcgcgaata ccgcaggcat gcccatcgtc agcaggtaat acaggccgcc
      721 gcccgcagaa aacgcgatag cggtaccgaa cgtgaccagc aaacccagga acatccagcc
      781 aaaggtcttg gcggtatata cgttcagagg ctcggaggcg agaccgccct ccatccactg
      841 gctgccattt tcctggaaca tagggtcaag gccctgttgc tcattcatgg atcaatccct
      901 catttctgtt gtgtatgggt ggaatgtgcc gtattgcaca tgacaaaagt atagcatgct
      961 caacgcccgt tttcaagata cagcgcgcaa aggtctgcca gaatatcctc cggcagcatg
     1021 ccctgctggg acaggcgcgc cgcgcaccgg tcggccgcga ggccgtgcag ataaacgccg
     1081 cacacggctg cctgcgccgg ggccaggccc tgcgcggcca gcccggcaat catcccggcc
     1141 agcacatcgc cgctgccgcc gcgggccagg cccgcgtttc cggtacggtt ttcgtacagc
     1201 cgcccggccg gcgtggccgt cagtgtgcgg tggcccttca gcaccaccac ggcgtccagc
     1261 tgtttggcca tggccagcgc cgtttcggca ggcgctgcca gcacctgctg cacggaggtg
     1321 tgggcaaggc gcgccatttc gcccggatgc ggcgtgacga tgagccgccc ggccgcgggg
     1381 cgcatggcct gcttttcggc ggcaaggctg cacaggccgc cggcgtccag caccactgtg
     1441 ccgcgggtgc gttccagcag aagcttcatt tcccgcgcgg tggtttcatc ggcttcacgc
     1501 ccgcagcccg ccaggcatac ggtggcggtt tcggccatgg aggcgagcgc gctttcatcc
     1561 ggcagcggca ggaacgtggc ctccagaacg cgcgcggcaa tgctttggat gacggattct
     1621 cccgcggcca gcgtaacgac gcccgcgccg gcgcgcagtg cgcccagcac ggaaagggcg
     1681 gcggcgccgc ggtaggccgg gcagccgcag acagccagca cacggccgta cgcgcccttg
     1741 tggctttccg ggggacgcgg cggcaaagcg gcgaacacca tttcattcgt gatgacagca
     1801 ggcataagaa cacttccttt tttacagcgg cggagccggg aatacgggac cataggaccg
     1861 cccggtgcac acgagccgcg ggcggggcat gcgggccgcg gcgcgggcgt cagctttttt
     1921 tagtgaacac gggcggcgcg caaagcgtga gcctgaaaat atggtatact ggtattgcag
     1981 actttgctat tattgtatca caaaatccgc ttctcagacg aacgggcccg aaaatatttg
     2041 cgagggctgc agtccgggac aaagcaggct tcccattgtt aaaaaacgcg gccgacgcac
     2101 cgacgcacag agattcagcc gcggcagctt gcgcagcgcg cttcccattg ttaaaaattg
     2161 ttaaaaaagg agagagggta tggacggacg tttgtgcagg ctggtggagg ccagcgaagc
     2221 gattgtattt ttcggtggag cgggcgtgtc gtgcgagagc ggcatcccgg atttccgcag
     2281 cacggacggg ctgtatcacc aggaataccg ctggccgccg gaggaaattc tgtcccacga
     2341 ttttttcgaa cgacggccgg aggagttctt ccgcttttac cgcgacaaaa tgctgtaccc
     2401 gcaggcggag ccgaacgcgg cgcaccttgc ccttgcgcgg ctggagcagg ccggtaagct
     2461 gaaagccgtc gtcacgcaga acatcgacgg cctgcaccag gcggcgggca gcaaaaacgt
     2521 gctggagctg cacggcagcg tgcaccgcaa ccactgcaca cgctgcggcg cgttttatac
     2581 gctggacgac gtgctgcgct ccgaaggcgt gccgcggtgc ggctgcggcg gcgtcatcaa
     2641 gccggacgtg gtgctgtacg gagaagcgct ggatgaaacg acgctcaacg cggctgtgcg
     2701 cgccatccgc cgtgcggacc tgctgctggt aggcggtacg tcgctgaacg tttaccccgc
     2761 ggcgggcctg ctgcgctatt ttacaggcgc cgcgctggcg gtcgtcaaca agacgccgac
     2821 cccggcagac gcccgggcag acctggtgat ccaggcctct atcggccgcg tactgggcgg
     2881 ctttgaggac tgaattgtaa aaaaacaaat aaacatacaa aaaatgtttt aaatcacttg
     2941 cttttttgtg taatatgcat gtatgataag aatgtagatt ttccacagaa cggagcgagt
     3001 ggagatgcgg gaaacagaga atacggcata cgcacgcaaa ag
//
LOCUS       DAWBSB010000257         3041 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_5226_length_3041_cov_3.510717, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000257 DAWBSB010000000
VERSION     DAWBSB010000257.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5226_length_3041_cov_3.510717"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          515 a          944 c          969 g          613 t
ORIGIN      
        1 gtgcacgctt tgaaacgcgc ggttaaacgc cgtcggagac tcatagccat accgcagcgc
       61 cacatccagt accctgcctc cgttctgaag gtcgaacgcg gccagcgtca tcctgcggcg
      121 gcgcagatat tccgagagcg gcacccctgc gatatacgaa aacatccggc cgaaatgata
      181 gaccgagcag cacgccagcc gtgccgcgtg ctccatatcc gccgtaccgg ccaaattcgc
      241 ttccagatat cccagcgcgt cgttcattct gtccagccat tccatccata tcagctcctt
      301 gcatccatca tagcagggcc gcacccggcg cgtcctctca ttttttgcag caaaatgcga
      361 ggcgttcctg ttttgcctcc gtcccctgta tgcgccgcac ggccagcgcc gggaatttcg
      421 ccgcctgcgg gatgcatgta gctgcgaaga gcaccgctgc gccccggccc gcctttttgc
      481 gccagcggcg cggtacggcc agccccagca gcaagcccag agcgcacatg cagcacttca
      541 actcggccag gtcccgccag cccgcctgct ttgtgtaatc ctctgccgcc tccaacaggt
      601 ttttcatccg cccacacctc cgctttattt tctttcagta taacagaaag caggcggagc
      661 gcaaccatat atgctatact attgataaaa acgccgggcc cccggcccgc aggcaaggag
      721 gatacaatga acgaagttac gactgcccaa atgaaagaac tggagcgcct ggccgccgcg
      781 gccgggctcc cgtacctgcg catgatggaa aacgcgggca cagctgccgc ccgggagctg
      841 atggcccgcg tgccgggcct gaaagccgcg gcggtgttct gcggcagggg caacaacggc
      901 ggcgacgggc ttgtggcggc gcgccgcctg caagacgccg gtgtggaggt gatagccgtt
      961 ctggccgagg gcgagccggc cacaccggac gccgccgcca atcgggaagc cgcgcaggac
     1021 cggggcgtgc atatacagac gctggacggc ctgacgctgc gggacgaggt gttcatcgcc
     1081 cgggcggacg cgctagtgga cgcagtatac ggcacgggct ttcacggcgc tctgcgtccg
     1141 gcgggacagc gtgcggccgc aatgctgaac agcggccgct ttgtgctggc gctggacgta
     1201 ccaagcggcg tgtgtgcgga caccggcgaa acagccgaag gcgcggtgcg cgcgcatgtc
     1261 accgttgctt tccacgcgcc caaaccctgc caccggctgg cagcggagca atgcggcacg
     1321 gtcgtcaccg cagatatcgg cattggtgca gcgttgggcg agggataggt ctgcggcatt
     1381 cttgtaaaac ctgtgccgcg cttatcgact tccccgcgcc gttgtgctat aatcggcttg
     1441 ttttgaaaaa aaggagtgta tttcggcgtg ggcatcaaaa ggatcggcgg agggatggcc
     1501 gtgaaccaga tcaccgaggg cgtgatctgg aagcagctgt tgtatttctt tttcccgatt
     1561 ttgttcggta cgttttttca gcagctttac aacacggcgg acgccgtcat cgtggggcag
     1621 ttcgttggca cgcaggcgct ggccgccgtg ggcggcgcta cgggctctct catcaacttc
     1681 ttcgtcaacc tgttcgtggg gcttgcctcc ggcacgacag tggtcatcgc gcaggcgtac
     1741 ggtgcgcacg acgcagagag cgtcggcggt acggtgcata cctcggtggc gctggctctg
     1801 gccgcggggc ttggtcttac agcagtcggc gtggcggccg cgccttgggc gctgagcgcc
     1861 atgggcaccc cggccgacat catggacttt gccctcacct atctgcgcgt ttacatgctg
     1921 ggcaccatcc cgtccttttt gtataacgtg ggttccggca tcctgcgggc cgtgggcgac
     1981 acccgccgcc cgctgtactt cctcatcatc gcgtgcctgg tgaacatcgc tctggacgtt
     2041 gtgctggtcg cggtgctggg catgggcgtg ctgggcgcgg ctttggccac catcctttcc
     2101 cagggcgtga gcgcggtact ggtgcttgtg tcgctgacgc gcgcggaaac cgtgtaccgg
     2161 ctggattgga agcaggtgcg cttccacgga cgcctgctgg gggatatcct gcgcgtgggc
     2221 gtgcccgccg ggctgcagtc gaacatgtac gccatctcca atatcctcat tcaaacagcc
     2281 atcaacggct tcggcacggc cacagtggcg gcctggacgg cgcacggcaa ggtggacggc
     2341 ttcttctgga tgatattggg cgcgtacggt atttccatca ccacctttgc cgggcagaac
     2401 ttcggcgcgc agaagtacga ccgcatccgc aggagcgtac gggtgtgcct tgcgcttggc
     2461 ttcggcagcg ctgtgctgat gagcgtgctg tactgctctc tggcgccgta tctgctgggc
     2521 ctgttcacca gggacgccga ggtggtgtcc ataggcctgg acatggtgcg gcagctggtg
     2581 ccgtattact tcacatatgt ctgcattgag atcctggccg gcgcggcgcg cggatgcggc
     2641 tgctcactgg cgccgatgct gatgacctgc ggcggcgtat gcgtgctgcg cgtggcttgg
     2701 atctttattc tgcttccgct gcggcccgag ctttccaccg tgctggtgag ttatccggcc
     2761 agctgggtgt tcaccagcgt actgttcatt ttttactatt tgcaaggagg ctggctgcgc
     2821 ggacgcatcc gtaagatggg ctatgcgccc gaggagcgcg gccgcaaaaa agaagccggc
     2881 gcgcactgac ggagtttgca cctttccgca acaggcaaaa gcacagggcc tttggccctg
     2941 ttcgccgcgc acagcgcggg gttattttaa atgaaaaagg ggatcaccac ccctttttgg
     3001 cggcattcgc cgcgtggctt gcactttgct gcgcaaagat g
//
LOCUS       DAWBSB010000258         3009 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_5301_length_3009_cov_2.276236, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000258 DAWBSB010000000
VERSION     DAWBSB010000258.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3009)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5301_length_3009_cov_2.276236"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1184..1193
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1933..1942
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          701 a          693 c          924 g          671 t
ORIGIN      
        1 atatattttt gatgaccgat tcgttgtcaa tttcaattac agagacgggg ataaactggt
       61 atctctgcaa gaggtaaaaa gttcgtcctt agctgaatat ggtttaccat ttgagccatc
      121 cttttcggat ggctcttttt ttgacggaaa gcgtcaatat cgcttttttc acgccgtata
      181 gtcaggattt tacgcagaat gctcccggaa ttgattgttc ggcttgtttt ttatgcttgg
      241 atacactaca atggataaaa aggatgggcc atttttcaca aaccggcccc ggcaaggagg
      301 catgtatatg ccgttttctg aagaaaatgt gctggatttt gtaaaatggc tcgaaaagtc
      361 gtattttgta gaacgggctc tgggaaggat tgaaaagctt ctggcaccgg aatttatgat
      421 ggtgggaacc ggacggcggg aggtctgcta tgacatgccg cagatgaggt tcctgctgca
      481 gcaggagcaa agtggatttc atgatacgtt tctggtgcta agtgcccatt acagcgtccg
      541 aacgctcaca ccggaggtgt tttccgtgtg cggtgagctt gtcgtgcgcg aaaacaatga
      601 atcctgtgaa atgctggatc ttcctgtccg cgttacgatc attttgcgcg cggagcaggg
      661 atgtctgctg ctttcgcagc tgcatttgtc gctgcccagt cccgaccagg atgacgcgga
      721 attttttccg cggctgttgg tgggggagaa catctccaca ctgcgcaggc ttgcagacga
      781 gcgctccgca gagctgcggg agcgcaaccg tgacctcgat gccctgatga acaatatccc
      841 cggcggcgtc atgtgctgcg acgccacgga cgaactgaac cttctgcagt tcagcgacgg
      901 acttctgtca atgttgggtt atacacgcga ggagctgggg actgttttcc ataaccgatt
      961 ctccgagatg atttacgagc cggatattgc acctacatgg gaggctgtgc atgcgcagct
     1021 ggaaaaaggc aatacgaagc tcattgagta ccggatggcc cgcaaggatg gtggtttgat
     1081 ttgggtgcag gacagaggcc agctcatgcg ccgggaggat gggcgagagg tattctactg
     1141 cattctgttg gatattactg aaacaaaaaa ggcgcaggag gatnnnnnnn nnnggataca
     1201 ctgactttct cgcccaactg ggaaaagaaa tttggctatg agccggtgcg ggaaaatgtt
     1261 catgcgcacc tgctggacaa cccgcatctg catcctgatg atgtaccact gctgcggcgc
     1321 aagctgtcgg atattctgga aagagaaccc tatcaggagg cggaacttcg cattcagaag
     1381 cgggacgggg gttatttgtg gtgccgtgtg cgtgcgacgc tgcagaaaga ccgcgcgggt
     1441 gaaccggcca aagcggtggg cgtgattttg gacatcgacg cggaaaagcg ggaggcccag
     1501 cgcatgcggg agcgtgcaga gcgcgacacc ttgacggggc tgtataacaa agggaccatg
     1561 gaagccaagg cagaccacgc gttgggtgca ctgatgcccg gggaaaacgc cgtgcttttg
     1621 atgatcgaca ttgacaattt caagcaggta aatgattttt acgggcatct gagcggtgac
     1681 gtgatgttga cggaggccgc acgtatgctg caggatatgt tccgcgcaag cgatattctg
     1741 ggacgcatcg gcggggacga attcaccgta ctgctgcgtg gttccggggc gcaggccata
     1801 gcgggccgca aggcgcagga ggtgttggac gcttttgcgc ggctcctgcc ggcacagggc
     1861 ggcgggccag ttttttcctg cagtgttggc atcgctcaag cgccgcagga tggtaccgat
     1921 tatctcacgc ttnnnnnnnn nnctttataa gaaggcagat gcggcgttgt accgagccaa
     1981 aatgcagggg aaaaatacct atgcgtttta tacccagttg ccgttggaag ggctgggagc
     2041 gcctacacag agcaccgtgg gacagacgat tgattccgag ctgggcacag gcgtgatgcg
     2101 cagctcgctg gcggagtatg tatttcacat tctctaccag tccgacgatg tggagaaagc
     2161 gattcccagc gtgctggaga tcgtagggcg gcatgtaggt gtgagccgcg tttatatttt
     2221 tgaagattcc gaggacggaa catactgcga taataccttt gaatggtgca acgacggcat
     2281 tgtgccgcag atagaccagc ttcagcatat gctggagggc gaactggcgg attattataa
     2341 gaattttaac gaagacggta ttttttactg ccgcgacatc catgcactgc ccccgaatca
     2401 ggtgcaggtt ctggaggcgc aaggcatcaa gtccatgctg cagtgtgtga tccgcgacga
     2461 cggcaagccc aggggctaca tcggctttga tgaatgccgg gaaagccgat tctggacgca
     2521 ggagcagacg gacctgctgt gcattgtagc ggaaatcgtg agcgtatacc tgctgaaggc
     2581 gcgggcacgc acaaggctca cccatgcgct gcagggcgcg caggcgattt tggactcgca
     2641 ggacgcatgg ctgtatgtca tccgaaaagg aacgtatgag ctgctttacg ccaataaaaa
     2701 aacaatgcag gatgtacccg gcgcaatgcc cggcgagcct tgttaccgtg tgttttaccg
     2761 ggcggacaag ccgtgcgtgc attgcccgct ggtaaagctg gcccccgaag gcccggaccg
     2821 cgtcaccgcg aggctgtaca gcgaggttct ggacatggaa gtaacggctg tgggcatgga
     2881 aatcccctgg gcgggcggag aggatgccta tctgatggcc tgtgaaaaag taaaaactca
     2941 gaagaaaaag tgaactgaaa aacagaaaag gcgcccggct gcggaaaaat aagcggccgg
     3001 gaatttttg
//
LOCUS       DAWBSB010000259         2989 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_5342_length_2989_cov_2.339127, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000259 DAWBSB010000000
VERSION     DAWBSB010000259.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5342_length_2989_cov_2.339127"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    806..815
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          610 a          808 c          945 g          616 t
ORIGIN      
        1 ggtgaacccc tttttcattt aaaataaccc cgcgccgtgc gcggcgaacg gggccaaagg
       61 ccctgttcgt aggcagcttt tgcgccaagc tgaaacccca gccttttggc tggggttttt
      121 gaatggagcg gattacgagg ctcgaactcg ccaccttctg cttgggaagc agacgctcta
      181 ccagatgagc taaatccgcg gagcatttat tattttaatc gcttcgcggc attttgtcaa
      241 tgcattgcgg gcaaaaaata gagcggcacg ccgttttgcc ggttattctg aaaaaccgtt
      301 tttcagtgcg cgcacatagc gggacgcctt ggcgatctgc tcatagctga gctggcggat
      361 gtcatccacc agattaaacg cattgatttt ttcctcgcgg tacatctgct ccagaaaacg
      421 gtggtattct tcgtccagaa cgatatccac catgcgcttg ccgtgggcgc tgagcgaacg
      481 gaacttgcgg atgcgtttga tctcctccga gctgaagatc tcgcttggat gggtgttcag
      541 caattcgtcc agcgaacagt tcagcacgcg ggtaatggct gtgatcgtct cccaggtggg
      601 gttctgattt tctccagaaa acaatttcga caccgtgcgg tacgggacgc cggactgctc
      661 ggcgatgctg acgcaggaaa gattgcgcac ctcgcggatc tcattgagct ttcgaatgtt
      721 cataaatttc tccttgtccc gcaatatttg ccttttatgg taagtaaata ttaccttaaa
      781 aggtaatatt tgtcactgat atcaannnnn nnnnncgctg cacatcctgc ggcattttca
      841 tttttgcctg cttgtgtgcg tgcaacgaac acaggaaagg tgagactgct ttattttgtc
      901 gttttgttta tgattttgtc gattttacaa aaaatggtat ttttcacatc tgaactgcga
      961 taaaataaaa gcgtagattt accgcgaaaa taaagggaaa aggggttcat ttcgggtatg
     1021 aggtatacgg tggtgtgcga gtgttcgttc gatgatgtga atggggaata tgccacctat
     1081 gcgcttgcct ggggcgaggc atgtgtgcat gacgtttcgg tggacgcggc ctttgtccag
     1141 cgcgtggcgg acgcgctgaa cggccaggac gtaccccccg agcatgtgta caacgtgctg
     1201 gacaactttt acggtatggg cgaagccgcc ccggtcaccc gccgctggtg ggaatgaacg
     1261 gcaggcagca gcccgccccg gccccgtttt gggggttttg cacaagtcag cttgccaaaa
     1321 aaggcgggcg aaaaagcaaa acttcaggaa cattttacga aaatttgaca gcggcattcc
     1381 ctttgtgcgt ccattcgttg taacgccgcc gacactgtgc tacaataaat gacagtgtga
     1441 gccgggcgtg cagggcgaag gcgcgcttgc cgtgcggctc tgggaacgtg aaattttcct
     1501 ggaggcgaaa caatgacgta cacgcgcaag aacaaaggcg tttgctctct ttccactgaa
     1561 gtgacgctca cagacgacgg cgtcatcgaa tccgttacag tcgtgggcgg ctgcaacggc
     1621 aacctgaaag gcatcagcgc cctgctcacg gggctcaggg cggaggacgc catccggcgc
     1681 ctcaagggcc ttacctgcgg cagcaagcac acctcctgcc cggaccagat cgcgttggcg
     1741 ctggaagaag cgctggcaaa atgaaccatc cataagatac caggaacatc aaaaacacgg
     1801 gcccggcagc ccgtgttttt ttcggtccgt ttgcggccat acttcccctg taaaggagcg
     1861 ttcggacgga tgaattacac tttcagcgga ttttcaaagg cgggcaacgc cgtggtgcgc
     1921 ggcgccattg cggcggcgca ggacatgggc catacttacg ttggcaccga acacctgctg
     1981 ctggcgctgg tgcgggacga cacgggcgaa gcggctgctt ttttgttgga aaggcaggtc
     2041 tacggctatc aggtggggcg gctgctgcgc gagcaggtgg gctgcggcag gcgcacgcgc
     2101 ctttccccaa gggatttcac gcccgcgctt tccaaatgcg tggactgcgc ggtgatcgag
     2161 gccaaggccg tgtccgacgg gaaggtggag ccggtgcatc tgctggccgc gctgctggag
     2221 gctccggcaa cggcggggcg cattctggtg cagcttggcg tggagcccgc cgccgcggca
     2281 aaggaatgcg gccggcgcat gggcaagttc ccgctgttcc agcagacgcc ccggcccgta
     2341 acgagccccc ggggcgcggc gcgcgccgcc gaaaaatacg gcaaggacct gacgagcctg
     2401 gccgagcagg acaagctgga cccggtgctg ggccgcgaca gcgaattgct gcgcatggag
     2461 cagatcctgg tgcggcggcg caaaaacaac ccctgtctgg tgggcgagcc gggcgtaggc
     2521 aagacggctg tggtggaagg gctggcccgg cgcatcgccg cgggacaggt gccgccccag
     2581 ctccggggca agcgcatcct ttcgctggac atcaccagcc ttgtggcggg caccaagtac
     2641 cggggcgatt ttgaggagcg gttcaagaac gtattgctgg atgtgcagcg cgccggggac
     2701 gtgattttat tcatcgacga ggtgcacgcc atcgtgggcg cgggcgcggc ggagggcggc
     2761 atcgacgcct gcggcatcct caagcccctg ctggcgcgcg gggacatcca gctcatcggc
     2821 gcaacaacgc gggtggaata ccgccgccac attgaaaagg acgcggcgct ggcccggcgc
     2881 ttcggcgcgg tggaggtgga ggagccgggc caggagaccg ccgtggagat cctgcagggg
     2941 cttgcgccgc gctacgcggc gcatcacgga gtggagatcg caccggagg
//
LOCUS       DAWBSB010000260         2971 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_5375_length_2971_cov_3.614198, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000260 DAWBSB010000000
VERSION     DAWBSB010000260.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5375_length_2971_cov_3.614198"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          489 a         1024 c          893 g          565 t
ORIGIN      
        1 gccatggcca gcagcgaaat gctgatctgc tcgcccgtgg ccagcagcat atccatctcg
       61 cgctgggacg ggtcgtgggt gatctcgccc gctttggcga tgaggtcgtc ggtggtatcg
      121 ccctgggcgg ataccaccac caccacgtcg ttgccggcgg cgtgcgtgtt cgccacgatg
      181 cgcgccacat taaaaatgcg gtcgcggtcc gcgacggagc tgccgccgaa tttttgtacg
      241 atgagtgcca tatctgttct cccctatccc tctatcaaaa gcaggggcgc ggcatacgcg
      301 ctcctgtttt ttcattttac cggcgcggcc gttcagacga cctttgcccc ctcgttgtcc
      361 gccacgaagc ggtgcagtgt aaaatgcgcc agctcctcgt cgctctccag cgccgcctgc
      421 gcgcgctcaa aaaacacgtt cgcttcggcc gtgcgcacaa cggaaaggat ggtcggcccc
      481 gcgccgctga taaacgtggc cagtgcgccc aggccgtccg ccagcgcgaa cacagcctcg
      541 ccgccctcga tgagcggcag gcggtactgc tggtgcaggc tgtccttggc ggccacgggc
      601 aaaaagtcgt accgctcctc gcagaaggcc gttgccagca gcgccgcgcg ggaaagattg
      661 tacacggcgt ctgcgtggga caccattttg ggcagcgctg cccgcgcctt ttccgtcagc
      721 agcttgaaat ccgggataaa tgccgcgaag gtgatcccgg gcgcgatggt ctttttcagc
      781 gagtagacat gcccctcgtc ataggccgaa accacgaagc cgcccagcag cgccggggcc
      841 acattgtccg ggtgcccctc cagcttggtg gcgaacgtca ggatgtcctt ttcctccagc
      901 gggccgccca gcagggcgtt ggcgcccaga atgcccgctg cgatgcacgc gctgctggag
      961 cccaggcccc gcgccatggg gatggggttc gtctgcacga tgcgcaggcc gtggaacggc
     1021 ctgccgcaca gcgcgtatac ctccttggcc gtgcggtaga tcaggttgcc cggcgtgctg
     1081 ggcacgaacg tgccgtcgga cgacgtgatg tccacgccgt caaattcctc aaacgccgcc
     1141 gtattgtaca gcgagagggc aagcccgcag gaatcgaagc ccgagccgat attcgcgctc
     1201 gtggccggaa cgctgacttt taccataccg cgccccctat tccgccatcc acttgagcgt
     1261 gttgcccacg cgcacgccgg cggcccgcgc cttggcgcac agctccgcaa gacgcccgct
     1321 tgtcacgccg cgcagcgtaa acacggcctc gccgtccgag gtatccagca tttcgatctg
     1381 ctggccgaac aggtcctttg ccaccgcctc ggtcgcgttt tccagccgga cgtaatggtc
     1441 cgccggcgtg ctgtcgccgt aaaggccctc gatgggcgcg gattcctgcc agaacaggct
     1501 gtcgtgtatc ttcacgccgt ttttcagcgc gtcgataacg tccgccacca cggcgctggc
     1561 cgtgggcagc ttacccgcac ccttgccgta gaacaccacg tcgcccagca tgtcgccctt
     1621 caccagcacg gcgttgaaca cgtcctccac gcccgcaagc tggttttccc gcgggatgag
     1681 catcggctct acggccagcg ccacgctgcc gtcgtccccg cgccgcgccc atgcgatgag
     1741 ccgcaccgcc gcgccctgcc gcgccgccag cgcgatgtcc ttcacagaca cgtcccggat
     1801 gccccgggtg ggcacgttct gcgggaaaaa atgccgcccg aaggcgaggc tggccagaat
     1861 ggagatcttt cgctgggcgt cgatgccgtc cacgtccgcg gaagggtcga tggtctcggc
     1921 gtagcccagg ctctgggcga gcttcagcgc cgcgccgaaa tccatatttt cccgcgccat
     1981 tttcgtgagc atgaagttcg tggtgccgtt gacgatgccc gcgatctcgc tgatgacgtt
     2041 ggccgccata cattggtgca tgggcgtgat gatgggcgtg ccgccgccca cgctggcctc
     2101 gaacagaaag cacacgcccc tgcggcgtgc aatggccagc agctctgcgc cgtgggtggc
     2161 caccagctcc ttgttggagg tgaccacatg gcggccgctc tccagcgcgg atttcacata
     2221 aaagtacgcg ggcttcaggc cgccgatggt ctccaccacg gcggtgacct ccgggtcgct
     2281 gaggatcacg tcgatgttgt tgacgaacag cgccgcgtcc gggtgggacg aaaagtcccg
     2341 gatgtcgcag atgtatttta ccgtgacggg ctggcccgcg cgccgctcga tgctggcggc
     2401 gttgcgccgc agcacctcca gcacgccgct gcccaccgtg ccgtagccca ggatcgctat
     2461 ggttgccatt gttctccccc tgtaccggga gggcgcgcgc cctccgcgtt ttgtttccgg
     2521 gcttttccga aagccctgct tttttacacg ccccggcgga tgtccaccac ggtgcgctgt
     2581 tttttcagct gcgccagcat atcctccagc gtgccctgca taccgcccgt gcggatggac
     2641 acggccacct gtgccgcgcc gttttccggc gtggcctggt tgatggtgac gatggacgcg
     2701 cccgccgccg aaatgcccgc cagcaggctt tgcagcgccc ccgtctcgtc cagaagcgtg
     2761 gcgttcaccg tcagcacttc cttgctgttt tccgcgtcaa aaatacagtc cttatacttg
     2821 taaaaggcac tgcgggaaag tccggccatc ttagtagcgg ccgaaacatt cttcgcgctg
     2881 cccgccgcca gaagctcctt ggcctttaat accttcaaaa acacctccgg cagaacgctc
     2941 acatccacca gcagaaaacg tcgctccaaa t
//
LOCUS       DAWBSB010000261         2926 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_5468_length_2926_cov_3.007663, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000261 DAWBSB010000000
VERSION     DAWBSB010000261.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5468_length_2926_cov_3.007663"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2335..2344
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          520 a          957 c          854 g          585 t
ORIGIN      
        1 tatccgctcc ttcgggcagc gtgccccaga tgaacatgcc gccctcgggc cgcgaagaac
       61 ggacctccgg gcacttttca tccagctgtt ccatcatcag cgccgccttg gccgcgtaaa
      121 ttttgcgtat ctcccggatg tgcgcgtcca tatccgtgcg cgtcagcatg gcctcgcata
      181 cacgctgcgc ccacacgttg gtgtgcacgt cgctgcactg cttcgccacc acaaaggcgg
      241 ccatcagctt tttgtcgcag acgcagtacg caaggcgcat gcccgggcac aaaattttgg
      301 aaaaactgcc cgcgtagata acggcaccct cggtgtccat gcttttgatg gacggcaccg
      361 gcccgcccgc gatgcgcagc tccccgtagg ggtcgtcctc cagcacgggc acgccgtacc
      421 gcaccgcaag cgcatacacg gccctgcgct tggcaaggct catggtcttg cccgtggggt
      481 tctggaaatt ggggatgcag tagaaaaagc gcggcttttc cggcgcggca aacacggcct
      541 ccagcgcggc caggtttacg ccgtcgtcct ccatcggcac gccggccagg cgcgcgccgc
      601 tgctgcggaa cgcgttgaac gcccccagga acgccgggtt ttccacagcc accacgtcgc
      661 cctcgttgca aaggcatttg gccgcgaaat ccaggatctg ctggctgccg gatgtaatga
      721 gcacgccgtc gttttccttc gccacccgtt cgcgccggtt ggcaaaggcc cgcacggctt
      781 cgcgcaccga gggcacgccc tccgacaccg aatactgcag cgcggccaca gggtcctgcc
      841 gcagcagttc gtccgaaaag cgcgcgatgt cctccgccgg gaatgaatcc gccgccgggt
      901 tcccgcccgc aaagctgatg agcgtcgggt cgctcatctg cttgagtatc tcgcggatga
      961 tcgacggctt catattgcgc atcttatccg agattttgaa ttccatgttc tttcccttcc
     1021 ctttccgtat cctttggccg ccccaaaagg cgggcggctc attccttccc gtctgttttg
     1081 cacgcgccgg ggatggccgc gtagctgtgg tcgatggtga ccacggcgta atatttcgtt
     1141 tcgcccgcgt cgtgcgtctc cacaaggctc tggatgcgtt tgcgcagcgc ggcgtccgaa
     1201 agcggaaaat tgtgcggcac cacggtgtcg aaaatcaaat ttgtgtggga cggcccctgc
     1261 accatgcgga aatcgtggat ggaaagctcc ggcgaaatgc tccgcaccag ctccttcacc
     1321 cactcccggg ccgtgcccac cgcgtcgtcg cccgtcagga tggggtcgta atggatgatg
     1381 aggctgatgt ggtcgttctc ctggaaatcc cgttcgatgt tgtcgatgat gtcgtggctc
     1441 tgcatcacgt cgttttccgc cgccatctcc acatgtacgc tggcaaaacg gcggccgggg
     1501 ccgtaatcgt gcaccatcag gtcgtgggtg cccagcacgc cgtcgtagct catcaccttt
     1561 ttgccgatgt aatccgtcag ctcctcgctg ggcgcctctc ccagcagcgg atcgaccgtt
     1621 tccttcagga tgcccacgcc gctccacaaa atgaacagcg ccacgccaag gcccatccag
     1681 ccgtccagct ccagccccgt caggcccgag accacgctcg ccgccagcac cgccgccgtg
     1741 gagatcacgt cgttgcggct gtccatggcc gtggcttcca gcgcggccga gccgatgcgc
     1801 ctgcccacct ttttattaaa ggccgccatc cacagcttca cgccgatgga gagcagcagc
     1861 accgccacgg tgacaaggct gaactccacg gccgtggggg acaggatctt gccaatgctg
     1921 gaacgcgcca gctccacgcc gatgacgagg atcatcaccg agacggccag ccccgccaga
     1981 tattcgatgc gcgcatggcc gtaagggtgt tcgtcgtccg cgggctttgc cgaaagccgg
     2041 aaccccacca gcgtgatgac gctggacgag gcgtcggaaa ggttgttcac cgcgtcggcc
     2101 gtaatggaga tgctaccgaa cagcgtgccc accgccagct tggccgcaca cagcagcacg
     2161 ttgcagaaaa tgcccaccgc gcccgccaaa ttgccgtaag cggtgcgcac ggcagggtcg
     2221 ttcacgtccg tgctgttttt gataaagcgt tttaccagaa agtccgtcaa agccgccgcc
     2281 tcttttccag aattatacaa atttcagatt ctttttatta tacggcgaag gaggnnnnnn
     2341 nnnnaggcaa aagtgcaacc cgtttgcagc ggctgaccgc cggtggccgc cgctttgccc
     2401 aagctgcgtc cgcttttctt gggcaaggcg acggttttcc ggcggcgtgc tgtgcaagcc
     2461 ctttgctttt tggtatgata cagtcaagaa gcagctttac cgctgttcac atttacatca
     2521 ggaggtgctc aggatgacaa cagcaaggga cccgcagaag aaaaagcgcg cgccctcgaa
     2581 gggccgggtg ctcctgtggg ccggcgtttt tctcttgaat ttcgttttgc tcacagcggc
     2641 ggtttccctg gcggtattcc cgcaggcggg cccgctgacg gccgagcggc tggcccggct
     2701 gccctatttt gaaggctgcg agcttctgga cgtgcacacg agggcgggcg gttctgcgcg
     2761 gctgttcggc caatcggacg cggactgggt gctttaccgg aacagcgccg gcgaaacgcg
     2821 ccttgtgggc gtcagctgga gcctttacac ctcccgcttt cgcatcgacg aaaaaaccga
     2881 cgccgccgtt cccggcgaca ggctttatca caccgctcag gttcag
//
LOCUS       DAWBSB010000262         2898 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_5544_length_2898_cov_1.965178, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000262 DAWBSB010000000
VERSION     DAWBSB010000262.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5544_length_2898_cov_1.965178"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    463..472
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2202..2211
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          698 a          761 c          847 g          572 t
ORIGIN      
        1 tcccaaggat gataagtact gcatccgccc tcatcagctc atcggcaaaa tgtgcgagct
       61 ggccgggccg gacgcgcttt acgtgacgga cgtcggccag caccagatgt gggccacgca
      121 gtacatccgc catgtaaaac cccgcagttt tctgacgagc ggcggcctgg ggaccatggg
      181 ctttggctac ggcgcggcca tcggcgcaaa gctggcggtg ggcgaaagcc gtccggtcat
      241 ccacattacg ggcgacggtt ctttccatat gaacctcaat gaagcatgta cagccgtcag
      301 ctacgagctg cccgtcatca cggtcatttt caacaatacg gtgctgggta tggtgcgcca
      361 gtggcagacg gcgttttacg gcaagcggta ttccagtaca gacccgcacc gcaaaacaaa
      421 ttatgtaaag ctggcagaag ggtttggcgc gaaaggctat cannnnnnnn nnccgaagcg
      481 ctgaaatcga aagggccggt gtggctcgaa tgtgtgatcg acaaagatga aaaggtgctg
      541 ccgatgatcc cggcgggcgg cagtatcgaa gatatgatca tggattaaag gaggcggcag
      601 tatgcccaga gaagtattga gcatccttgt ggataaccat tccggcgtgc tgacgcgcgt
      661 ggcgtccctg tttggacgcc gcggcttcaa catcgacagt ctgacagtgt ccgccacgga
      721 tgaccccgcg atctcgcgta tcaccattgt ggtgcacgac gacgccaagg tgctggaaca
      781 gatcatcaaa cagacggcgc gcctggagga aacgcgggaa atttttccgc tggataccgc
      841 ccacagcctg ctgcgcgagc tgcttctggt gaaggttgag gcgggcgagg aaaatcgttc
      901 agctatacgg gagatcgcca acatttacaa agcgaagatc atcgaccttt ccatcggcag
      961 tatggtgatg gagctgacgg gcgaaccgga aaagatcgac ggctttctgg atgtgctttc
     1021 cccatacaaa atactggaaa tgtgccgcac gggcatcacg gccctggaac gcggcggcgt
     1081 gcaccggcac gcgtgagcgt tgtatcatcc ggcgcggcac ccagccgtgc ctcacggtaa
     1141 ataatatata gagggagaaa tgtaaaatga tcaaaaagta ttatgacacg gactgcaacc
     1201 tgggctttct ggacggaaag accgttgcca ttatgggtta cggcagccag ggccacgcgc
     1261 acgcacagaa cctgcacgag agcggtgttg acgttgtcgt tggcctgcgt aaggacagcg
     1321 caagctgggc gaaggcggaa gcggccggcc tgaaggtcat ggaggtgcct gaggcagcca
     1381 aggctgccga cgttgtgatg atgctcgtgc cggatgagat cgccgccgac atctataacg
     1441 agcaggtagc tccgtacatg aaagagggcg acgtgctgat gttcgctcac ggctttaaca
     1501 tccactttca gttcgtaaaa cccgctaaga acatcgacgt gatcatggtg gcccccaagg
     1561 gccccggcca caccgtccgc agccagtacg tggagggcaa gggcgtgccc agcctgattg
     1621 ccgtggagca ggattacacc ggccatgcca aggatatcgc gctggcttac gccagcggca
     1681 ttggcgcagg ccgcgccggc attctggaaa cctccttccg tgaagagacc gagaccgacc
     1741 tgttcggcga gcagtgcgtg ctgtgcggcg gcgtgaccga actgatgaag gcgggcttcg
     1801 acacgctggt agaagccggc tatgagcctg aaatggcgta ctttgaatgt atccacgaga
     1861 tgaagctgat cgtcgacctc atcaacagcg gcggttttgc catgatgcgc tattccatct
     1921 ccaacacggc ggaatacggc gattaccgga ccggaaagcg cctcatcacc gaggagaccc
     1981 gcaaagagat gaagaacgtt ctgacggaga tccagaacgg cacgttcgcc agtgagttca
     2041 tgcaggaatt ttctccggct ggcggccgta aagcgaaatt ccttgctacg cgccgcgtag
     2101 agagccagca tctgctggag aaggtgggcg cagagctgcg caagatgatg agctggctga
     2161 aaaaataatc cgaagcgaaa agagccgcgc cgcgcgtccc gnnnnnnnnn naaaagaatc
     2221 cgaactggag gcatagggat cctatttaca aatcccgagc gatgtccacg acgaggtgac
     2281 ctacttcaac agcacaaagg acggacgtgg ctacaaccta ttgtatctga acgcattgta
     2341 tgatctggag agttaacttt atctggaagc cgtcatacaa aacgggcgag aagagcatga
     2401 gtcccaggca cttgtggaaa tggttgaccg atcggaactt gcagacctag ttattctgat
     2461 tgcggatcgc ggttacgagg cgtacaatag catggcgtac atcgagcaga agggccggaa
     2521 ttatctggtt cgcgtaaaga aaaagctggg tatcctttca ggtctccgac tgcctgacac
     2581 gccggaattt gaagcggctt tcagctgttt cctctccaaa cgcctcacaa accgtatccg
     2641 ccaggagccg cagaaatatc gttggctccc caataaggtt cattttgact acatcagcga
     2701 tgcaagtgat atggctacat ccggtttcct tccgtatggt aagcactaac gcacttccat
     2761 gcgaaaaagc gtccattcat tatgcaggat atcttcgcca gaatgacgct ctacaatttg
     2821 gcgtccctgc ttcgcctgca tgccagtttt atgcagctga agggcgaata tctctaccgt
     2881 gtgaacgatg cttttgcc
//
LOCUS       DAWBSB010000263         2860 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_5627_length_2860_cov_2.946881, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000263 DAWBSB010000000
VERSION     DAWBSB010000263.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5627_length_2860_cov_2.946881"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    229..238
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          603 a          865 c          769 g          613 t
ORIGIN      
        1 gtctttccgt tgcgcggcag ccctgcatgg agttctacgg agtccatttc acgctgccgt
       61 cggacgtgga ccgccttccg ctgcccgagg ttgccaaaat cgaaccgccc ggcaggctgg
      121 agcctctgtt caaatctctg cttgagatct acaagcaaaa aacatatctt tatcaatggc
      181 ggcaaaatct tctgctcgag cagatcttat gcgaaatact ctccgcacnn nnnnnnnngc
      241 atggggaaaa tgctcccgca gagcttatcc gtattaaaaa agtgctggag catatccatg
      301 aggaccctgc ccgtccgttt acgctggaag agcttactgc ccgctccggc gtaaaaaaga
      361 cgctgttcct gcaatcctcc cgtcgggtga ccggaacaac gcccaagcag tatattctcg
      421 ggctgcggct ggaatatgcg cgggatctgc tgctggaaac agacgtcccc gtcgcgcagg
      481 tagccgaacg ctgcggcttt tccgattcct tttatttcag ccgctgcttc aaaaaccatt
      541 tcagcacttc gccccggcaa tatcgaaagg aacacgctta gattccaaaa agccaatttg
      601 cgcatgtttt ttgccaattt cagcaaagaa tctgcgcaat tttttttgta ttctgaaggt
      661 agcgttcctc ccgcgggaga aacacggaaa aaacaagggg gccataacta tggaaaaaat
      721 caaattgggt atcatcggcg tgggaaacat gggcacctgt cacattgaga cgacagcgcc
      781 gtgaagctgc cgcccgaatt ttacactatg tttgcacgtg ctttcttctc acaactgatg
      841 aagcgttacg gcggtttcat tttccgccgg ctgtttgtat ccatcccggt ggcggccgca
      901 caaaaaacgc aaagcttttt acccctcctt tggcggcatg cttgagccgg aaaacatttt
      961 ttctttattg aaactcggcc tgtgcgttca taaggccggc tatggaagat ctgtttcaac
     1021 tgcacttttt cctgtgccgc tcagcgcatc gaaagttcct caatgtaatc gttataccat
     1081 tgtacggcag ttctggccgt cctgcgaagc tgcatgccct ttccggccag cagtacaagg
     1141 cggttgtccg cttctccctg ccagggcgtc agacgaacct ccggcccggt gcagtcgacg
     1201 taaaagatgc cctccggcac gcgtacaaag cctttgatac gccatgtttc tcccgcaagc
     1261 tgtacaaggc acttttgcag tgccgcaaag ggcatcgtgg gacggatctg cagcaccgct
     1321 ttttgcaggg tgatatccgg catgtccaat acctcatcca catccacatc cggtacgatc
     1381 agggaaagcc attccggctc gaaccgtccg aactgcgtgc ggcggatgcg ggccgccggg
     1441 ttggcgcgca ggataagcgc ttttgcttca gcgcactgcg cttcggtggc aaggtctgtt
     1501 ttgttcagaa gcacgagact ggagacagca agctgccggg ggcacgcccg ggcagtagag
     1561 aaaacctttt caagccgcgc ggcatctgcg acgcacacgc tgccctggta ggaaacagcg
     1621 ggaaattggg ccaaaacccg gcgcacgttg gtggggtcgg ccaagccgct ggcttccacg
     1681 agcgcaacat ccggtgcatc cgcggccgcc tgctccagtg cggcctcaaa ttggtccaaa
     1741 cggcaggcgc agaaaatcga gccgttgctg atctcatcca acgccgcgtc cagctcccgc
     1801 agcagcgtgc cgtccacgcc gacgcgtccg aattcgttga tgatgagccg cagccgccgg
     1861 ccggcaaact gccgggcaaa atttttgagg aaggtggttt tccctgcacc caaaaaaccg
     1921 gtgatgagat acagttccat tatatcgcgc ctttcttagc tgaaaagagg ctctagacct
     1981 cccattccgt tttttgcagc agcgcacggg cctgctctgt ggagaacccg cggttgaggc
     2041 gcgtttccag cgaattgtcg cgcgggttca cgccgcattc tgcataaatc tggtttgcac
     2101 cggccatcag gcaaagctca tccggctcat gtacgcacat agcgcgcggc ggccgggcgg
     2161 tgagcgtggt aacggcgcag atcttggcaa gctgcgcggc cgatatctcg ccctgcgagc
     2221 ccaacggcgt accgggcacg gcaatgcgct tcatcacggc catcactccc acggggtaat
     2281 cacggatgcg cagcatttcg tcgaccagtt cctgatgcgt gtgctccggc ccgatcggtt
     2341 ctacacaata atacagctcc agccctgccc cgcgcacggc atccatcgtg cggatgcgcg
     2401 cctgcggacg tgcattcgtg tcggtgccct cccgcagacg gtggatatgg tacacaccag
     2461 taaatcccgc ctctttcagg gctgcagcat aggccggacc aaaatccccc acattggcaa
     2521 caaggcgcat ctgcgggggc agcactgcgc gcactgccct gccgtatgcc agaaattttt
     2581 cctgggggaa ttccgcggtc gtcatcaaaa acaggtcttc gatcccgctg gcagccagct
     2641 cacgcgccaa ttccaccgtc tcttccagcg ttttgatgca ggctggctgg cccctgcgcg
     2701 cgtctgcggc aaggctgcag aatttgcagt tgacgccaca ggcctgggcg tcaataccga
     2761 tctgcgcaaa aataacgcca cggccacgga acgctgtgcg cgcatacgca ttggccgcac
     2821 ccagcagtgc ataatattcc gcactgccgg tggccacctc
//
LOCUS       DAWBSB010000264         2829 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_5702_length_2829_cov_2.986662, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000264 DAWBSB010000000
VERSION     DAWBSB010000264.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5702_length_2829_cov_2.986662"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          602 a          926 c          715 g          586 t
ORIGIN      
        1 aagaagcccg ccgtgccgag gaagcgcgtt tggcggaaga agcccgccgt gccgaggaag
       61 cgcgtttggc ggaagaggtt caccaggccg aggaagcaca ggccgcatcg gctcagacgg
      121 gccccgtcgc agagggcccc acagaggacg ctgcgcctgc atctgtgact gctgggcaga
      181 cggacgcgga tgcaatccct ctctcctggg acgagccttc ggctgagata tccttctcca
      241 ttccggagga acccgcgccg cagcctgtgg ccgaggtttc tttctctatt cccgcagagc
      301 cggaacttca gcctgccgca caagcggttc cggaagcaca ggcgcagcca gcgcctgtgt
      361 ttccggaacc gacggcggac actgaggctg cccgccgcgc agaagcgaac gccatgcgcc
      421 tgcaagccgc ccgccatgca caggaagccc gccgctctgc cgagagccgt gccgccgaag
      481 aggctcagct tgctgctgtt gcgcgccagg ctgaagaagc acgtctggct caggaggcgc
      541 agcgagctca ggcatcccag actgctcagc ccgcacctca ggcggtacct tctcaatgcg
      601 ctccccagcc gtcgaaagcc gaacagcccg gtacaaacca gtgggtagca tggacgccca
      661 tccagtttga tattcctgct gaaccggtac cgcagcagct tgctgcgcct acgccagaaa
      721 ccacctggac ggctgcagca cccaatgccg ccttcaatga acccgcgcgt gtcgcacagg
      781 ccgctccggc acaggaaaag gctccgcgca aagaaaaacg cggttttctg ggcctgttcg
      841 gccggcgtaa gcagcaacct gcgccccaac cggaagcaga gccggttccg gcgcctgtgc
      901 aggttcccgc gcctccccct cagccggcac ccacgccgca gcccaccgtg cagcagcccg
      961 agggcgacca gcgcgtaaag ctgaatgtga agccgatctc ctggcctgcg ccgcctccgc
     1021 cttcacgcaa ctttgtgcat ccgccccgct ctacgatgcc gtttacagcc atcacacagg
     1081 agcagatcca gcaggcgcaa gccgtacagc ggcaaaccgc gcctgcggca cagccaatat
     1141 ctgcggcagt gcgggaaact tcgccgcaga ccgttgctgc tcccgctcag cagcccacac
     1201 agcaaaccgg cgctgcagtt cctcctgttc agcagcccgc tgcaccaaag gcttctccca
     1261 tacagacggg ttctcccacg ccgtactggc aggagtaacg cagcctcacg ggagttggcg
     1321 ggaaatacag ctgttattgc cgcaaacaga ttttaaccaa cacaaaacgc ctccgggacc
     1381 acgacaggtc ccggaggcgt ttttggcttt tggcatccca tcgctgcggc atctaaatat
     1441 tcttccgagt ttcccatccg cattcgccac gcagtgcata ccccgctgcg cgagaccgct
     1501 tgcggtagaa tgagccccgg aaaaacatcc gtttttccgt tcatccgcct tgtcccgcct
     1561 catcttgtat tgccgaggat gcgatctgct ctctgcggac cgcatacacc aaacgccaga
     1621 cgccgttctc cagccgccac agcacccgca cagactcacg cctgccggag cctccggtgc
     1681 gcaccgctgt ggcacatacc gcatatttcg gagcggaaag catctgataa ctgtaaagcc
     1741 aatttccgtc ccctttttcc attcccacca ttgtttggaa ttgcttctct atgtgtgcca
     1801 gtcccggcgg cgccgggcag cagacagcac gctccagcat cgcgccgctc accgtgcagc
     1861 acagcgccag cgccgcagca agacccacaa accgctgttt cacatatcag ccctcctccc
     1921 ggacaggctc tccgagctgc ttctcacagc ccttgcacag gctccatccg tctcaatctt
     1981 caagtacggg cccgggagcc gaaacggcat cgtccgcgcg cttctaaaat acccctcagt
     2041 caacccctgc cggacctttt tgtgtattct gcgttcatta tactcaatat atttagtata
     2101 gtcaatgatt tgagtatgat aaagtgaagc aagaggtggg gggaatgatc agctacgagc
     2161 cgttttaccg cacgctgtac cggaaaaaca tcacagaata ccagttgatc ttcaagcacg
     2221 gcgtttcggc caataccctg caccgcatga agcatggcga gaccatcacg ctgaaaacac
     2281 tggataccct gtgctttatt ttggattgcc cggtgtcgga tgtgattgaa taccgcaggg
     2341 acggagaata acaaaagagg cgcgcctgta aaggcacgcc tcttttgtct gtttcacacg
     2401 ttttcagctg atggcttcgt aaacgcgctg cagctctttt tcgttttcgc tcacaagcat
     2461 caccggatgc gtataatcca ggctcatcag ccccagaata cttttcgcgt cggcaatgct
     2521 gccctttcca tcgtgcacac cgatctcatc cacacaatgg gtcgcggcat tcatgatgtt
     2581 tttaacctct gtgaagtttg aaattttgat ttgctttacc ataacgcttt tcctttctgt
     2641 aacaaacagg ctcttgcccg tgccgtaagg tgtcccatct aatttatgga acgcttcccc
     2701 tatccttaca tttggcatta tagcacaaat ttatacgttt gtcttgtgaa aatctaccaa
     2761 tgctggtact ttgtttcttc tttctcacta ttcaacaaat ctacattttc gaagaagctg
     2821 cttttgtac
//
LOCUS       DAWBSB010000265         2799 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_5778_length_2799_cov_1.990160, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000265 DAWBSB010000000
VERSION     DAWBSB010000265.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5778_length_2799_cov_1.990160"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1587..1600
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          701 a          734 c          535 g          815 t
ORIGIN      
        1 gaacccacac gaccagcttg gaaggctggg gttctaccac tgaactacac ccgcacgttt
       61 cgcgacggta gttattatag catggccgca taatttcgtc aagagttttc tgatttttac
      121 tcttcccttt ttccgtaaaa aaactcctgg caaaaacccg cataacaatg gcggcagaag
      181 cgcgttccgc cgccataatt tctattctgt aaaaacgcgc cttactcagg ccgcttctaa
      241 aaagcgtact gtgttcatca gcagtgtaac gccctccgcc cgtgtgatag tgttctgggg
      301 gcggaccgtg ccgtcttcat agccggataa cagtcccatt ttctgtgcct ttgccaacgt
      361 ttctctggca taaccgctga tccgcgccgc gtccacaaag tcggcctctt ccgccacaac
      421 ggaaattccc cggctttcga catatcgcac catcatagta gcggcctgct cgcgggtaac
      481 attggccaga ggggcaaatt tcgtctctga aacaccgtaa gccacaccgc tttcatatgc
      541 ccagttcact gcatcggtgt accaggttcc cggcttaaca tcctcaaggg gcgccggctc
      601 tgccgccgct tccccgcctt cagctctata cagcaccgtg gcgaacatgg cgcggttcat
      661 cttcccctcc ggctcaaaat gcgccagaga agttccccct gccaagccgc gctctgcagc
      721 ctccatcacg tcgtccagat accatgcatc agcgtcgata tcgaggaaaa ggtcgccttc
      781 aaacagcgca tacaccgtca tattttcttt cggcacaatg gtgtattccg gctctgtact
      841 gagtaaggcg ccgtctgcag aataccagcc gaccacaggc agttcacttg ccgacgtaag
      901 tgtggtttcc tccccccaga ggcaaactcc gccgccttcc acctgacccg aacgggaaga
      961 gtgcacggtt acatttgcag tgcggacacg ctctgccgtt acaggcaaaa gactcgcatc
     1021 gcgcagggtg tttgccgccg tttcctgcga aaaccgccat gaggaagaca tgggaatccc
     1081 gaaatgatcg tatatgatag atccataatt ctgcttcgag cgccaatcct gctggccgtc
     1141 aaaaatatag ttttttccgc ccagctttaa ttccgtccag ccgtgatgac ccgtccagtc
     1201 gccgattctc aaaatacccg tgtgcacata gctttctatc cccacatacc gggccataac
     1261 tgcaaacaaa atcccccagt cataacacac gcccgtacgg gctgtaagca tatgataagc
     1321 ccggtaaatc atgtccttgg gatatgcctc cggcaggcct gtttcgtatg taagcggata
     1381 cgtcagcgta aagcagtcgt aggccggggc ataatcctgc gaatagccgt cccagctgta
     1441 gtctatgttg ttcaccgccc aatcatacat ggccttgatt ctggtgtagg tatcctttcc
     1501 tgcatagggc gctgcaaggg cttccagcat ctcatccagt tctacatacc cggttttttg
     1561 cggatagagc ttttccgcgt tgagcannnn nnnnnnnnnn cctgttgctc tttttcttca
     1621 tacagtactc ctcccttaaa acagcagcta tactttgcgt acaatacaat gatagtacta
     1681 ttcatcccag attgtcaatc ttctcctcct ccattaagcg tcaaaagagg gaggcgtcca
     1741 ccattttctg ccatcggatt tttgaacaca tccaattcta tcatatttgt gtcatctcca
     1801 actttcaatt tatcgctctc ttatttcttt cccgtttttc tttcttcttc tggcagcttc
     1861 ctcagattca gccgaagcct tttataagat tggaatgggc ggaaatccat ttcagggctt
     1921 acacgccttg actggttcgg ctggcgttgc tatcttttcg atgactggaa aatcaaaagc
     1981 gtgaaatccc atttttataa tcaaatacaa ctaagccaca ctctttacaa atatagcctt
     2041 gaaaatctgt tcctgtaaat gcttttcttg caatcataac atctgcttca tttttaggat
     2101 ttaacgatat tttgtgtctt gtttgattga atgcaacaat attggttgtt tggagaaatc
     2161 cattttgcat ctcttttcca cacttagaac atttcattga acatctacct ctctctaaac
     2221 actatttgtt tgaaaccata attccatctg aattgctagc catttgatga atagtattct
     2281 tgcagtatcc ttgctaaaat ttgaggactt tcttcattta caacatgacc agtattttca
     2341 atcactttca tttctgaatt ttttatgttt tgcgataagt aatatgccga tcttaaattt
     2401 gcggtatctt tctttccaca aaggattaaa gttggggatt ttaaagtatt caccttacca
     2461 ctaaaatcta aattcttcat agtgtctccc aaagcgaaag tattcttttt gtcaaatgcc
     2521 atcgtttcaa aaatagattt tggtaaaaat ctgaaaatta gattttgaaa accaaacatt
     2581 gctttcggaa ttttatgggg cgttcctatt aaaactaatg tttttatttt ttgcgggaaa
     2641 tccaaagcat aatttaacgc caaaatacct cctaatgaaa tgccgcacaa atgaatttct
     2701 ccatcaattt ggttacagta cttcacaaat gaagagtaca gattttcata acttgcttct
     2761 ttcccttcaa gaatagaaga taaaccagga cacataatg
//
LOCUS       DAWBSB010000266         2768 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_5837_length_2768_cov_3.513454, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000266 DAWBSB010000000
VERSION     DAWBSB010000266.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5837_length_2768_cov_3.513454"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          606 a          741 c          878 g          543 t
ORIGIN      
        1 gcgcacaggg ctttgagaac attgaaccat gcaaaaccgc aatagatgcg gttttattga
       61 ggccgcttcg cggtgttatg gtatactatg accataggca aaaacagtgt ggtgatatag
      121 gatgtataag gtgatgatcg tggacgacga ggatatcatc gtcagcggcc tggtaaaggt
      181 gatgccctgg gcaaagtatg gctgcgaagt ggcggctacc gcgtccgatg ggcttgcggc
      241 gcaggccgtg ctgcgggaaa agcggccgga tatcctgttc acggatatct gcatgccggg
      301 cgcggacggg cttgcgctgc tgggggctgt acgcagcgag ttcccagaca tgcaggtgac
      361 gatcctgagc gggttcccgg attttgaata cgcccagcgg gccatcggcc tgggtgtggt
      421 gcggtatgta cttaagccca gcaagatgca tgagctggaa gaggcactgg ccgtcatggt
      481 ggaaaacctg ggcggcgcgc ccgcgtccgg cgcggtgcag tctgcggaaa atgcgcagaa
      541 tttcatcata aagaaagcgg tacagtacat tgagcagcat tatgcggaaa aactgacgct
      601 gcccgacgtg gcggacaagg tctacgtcag ccagtggcac ctctccaagc tcattgccaa
      661 gaacacggga cagagttttt ccgacctgct caacggcgtt cgcatcgcca aagcaaagga
      721 gctgctggcc gacccagccc tcaaggtctg ggaagtgagc gacgcggtgg gctttgcgga
      781 tgtgacgcat ttttcccgca ttttcaaaaa aatggagaat atgagcgcca acgaataccg
      841 caataagcag atcgcaagat gaccctgcgt taaaacaaaa ccgcgccccg tatgctgcac
      901 acggggcgcg gttttgcgcg ccttacacct cagacgcgcg cttggctttc cagcgtgcgg
      961 ccagacgcag caggccgaac acgggcaggc acagcagaaa ccagtaaaag ctgaacaggg
     1021 ggcatatctg tcccagcaca ttgccgaatt cccggctgta atcccatacc tgccagccca
     1081 gcgcccggtt caccaccagc cctacggcta attccgccgc tgtgatgaac gccgtgccca
     1141 gcgcggcctg cacggcgatg ttcatgccgg ggtatcgccg cgcgatccac accagagcca
     1201 gcacgcatat gccgcccagc agggccatgc tccagtgcgt gtgtccccgc gccaggatct
     1261 cgatacaggg atatccggtg gcacccaccg cgaaagccgt tgccttttcc ttcatttaca
     1321 catcacccgg gtataggatg cgccgttttt gcacggaata tcacaaacaa ttgcgccgcc
     1381 cattgctatt tgtgggggaa agatggtaaa atatataaga ccataaactg agaatggagt
     1441 agttatgcaa aagacaaagg tcagcgcgtg catcgttgtg tacggcggtg gggaagaagc
     1501 ggcacaggcc gcgcgctctc tggtggagca cacacgggac gcggcgcttt cgctcacact
     1561 ggtggacaac gcctcgccgg acggcgcggg cgaatggctt gcggcgcagc agcttggccc
     1621 cggagtgaag gtggtgcgcc tgcccgagaa cgtgggcttc ggcagcgggc ataacaccgt
     1681 gctgccacag ctggacagcg tttaccattt tgtggtgaac ccggatatca cgttggacga
     1741 cgacgccgtt tcaaagctgt gcgcgtggat ggacgctcac ccggatgtgg tgatggcgac
     1801 gccgcgcctg ctgttcccga acggggccga gcagtatacg gcaaagcgga ggcccagctt
     1861 tctggcgctg ctggcccggc aggttccgct gccgtttttg aagggcgtgg aacggcatta
     1921 tctgatgcag gacgaggatc tgtcctgccc gcaggagatc gagttctgca cgggctgttt
     1981 ttttgtgatg cgcaccgagg cgttccgccg catgggcggc ttcgacgagg gttattttat
     2041 gtatgtggag gacgcggata tcacccgcaa ggcgcaggaa tacggcaagg tgatgtacgt
     2101 gcccgaggcg catgtctatc atgcctggca ccgggacaca aagaaaaagt ggaagaattt
     2161 ctggatgcag atccgctcga tgctccgtta ctggcgcaaa tggggtttcc gaatggtttg
     2221 aggggcgggt gttttcccgg gaaaacacgg ggaagcctgc attgctttac gcatgcaggt
     2281 gtacagcaga gaaaaaagaa aagcaaagga gcaggaacaa tgaagggaat catactggcc
     2341 gggggcgccg gcacgcggtt gtatccgctg acgatggtca cctcgaagca gcttttgccg
     2401 gtgtacgaca agccgatgat ctattatccg ctgtccacgc tgatgctggc gggcattcag
     2461 gatatcctga tcatctcaac accggaggac acgccccgtt ttgaatcgct tctgggcgac
     2521 ggaagccagt tcggcgtgaa gctgtcgtac aaggtgcagc cttcgccgga cggcctggcg
     2581 caggcgttca ttctggggga tgcattcatc ggggacgagc catgcgccat ggtgctgggc
     2641 gacaacattt tttacggcaa cggcttttcc aggctgctgc gcgcggcggt ggaaaacgcc
     2701 gaaaacggac gcgctacggt gttcggctat tatgtgaacg atcccgagcg cttcggcatc
     2761 gtggaatt
//
LOCUS       DAWBSB010000267         2762 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_5853_length_2762_cov_1.875877, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000267 DAWBSB010000000
VERSION     DAWBSB010000267.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5853_length_2762_cov_1.875877"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    294..303
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    606..615
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1598..1607
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          556 a          858 c          685 g          633 t
ORIGIN      
        1 ccaaaagttc cgcatccacc ggcagcaggc catactcttt cgcatagcgc agcgccttgg
       61 ccaccggcag ttcagggatg gaatccttca attcctgaac gtgcgcttca ctcacttcaa
      121 acgtaagcag atcggggtct gggaaatagc ggtagtcctg cgcatcctct ttcgagcgca
      181 tcacatagct tttgcccttg gcatcatccc agcggcgggt ctcctgctcg ataacgccac
      241 ccttgctgat gacctcgatc tgccgctgcg cctcatactg gatggcgcgg tacnnnnnnn
      301 nnngcatctt ggcgtctgag atgccgagat aaagcaggat gtttgtaatg gcttccatgt
      361 acgcctttgc ttcctccgca cttcgcaggt ccggctcgga aaccatctca ataagcggca
      421 cgccgcagcg gttgtaatcc gccaacgttc cgtcaaagga atcatcgtga atgagcttgc
      481 ccgcatcctc ttcgatgtgg atgcgcgtga taccgatgcg ccgtacgtcg ccattcacaa
      541 ggatgtccaa ataaccgttt tcgcacaacg gaacgtcaaa ctggctgatc tgatacgcct
      601 tgggannnnn nnnnngaagg tccggataaa aatagttctt tcggtcattt ttactcacac
      661 ggttgatcga gcagttcagc gcatggccca tttttatggc gtaatccacc acctgcttgt
      721 tcagcaccgg cagcgtgccg ggcatcccca tgcacaccgg acagcacaat gtgttcacct
      781 ctgcgccaaa agcatttttg cagccacaga aaatcttggt ctcggtgcac agctcggcat
      841 gcacctcaag gccgatcact acttcataat ccctgtttat catctcgtca cagctccttt
      901 accgcaaagc cgcccctggc agtctcgtaa gttttcgcaa cgcccagcaa ttttgcctcg
      961 ctgaacttcg gcccgatgag ctgcaggccg atgggcaggc tgcccgcgcc gaacccgcag
     1021 ggtacgctga tgcccggcag gcccgcgata ttcacggtaa cagtgcagat atccgaggca
     1081 tacatctcca gcgggtcgct cgtcttttca cccaggcgga acgccgtggt aggcgtagta
     1141 ggcgtggcaa tgacgtcgca tttccggaat gcatcttcaa actcctgcgc gatcatccgc
     1201 tgcagcatct tcgcgcgttt gtagtacgcg tcataataac cgctggaaag cacataggtg
     1261 cccagcatga tgcggcgttt tacctccgcg ccaaaacctt cgctgcgcgt agaaagataa
     1321 agatcgttca aattgccctc gcgattgcct gaatagccgt attttacgcc gtcataacgc
     1381 gccagattcg aagacgcttc agcggaggaa atgatataat aggccgaaag agcataatcc
     1441 gtggaaggca aagagatttc gacgatttca gcgcccagcg ctttcaggtc atccactgcc
     1501 ttcaatacag cggccttatt ctccgcgctg acgccctttc cgaagtactc tttcggcaca
     1561 ccgacgcgca agcccttaac gcccgtcgtt atgcccgnnn nnnnnnngag ccgcccggca
     1621 cacgagtggt atcccaaggg tttttcgtgg gcttgaaata agaattttcg cacgaaccgc
     1681 ccatggcaaa ttcgtccatg ttcagcttgc cggggattac cgcgtctgcg gccgccagct
     1741 tctccactac cgtcgcatta tacggcggca caaaattctc cagcatccgg gaggcacagg
     1801 tcgttttcag gcccttggta cagatattgt cctttatccc cacaggaatg ccggccagca
     1861 cgcccagcgt ctcccctctg gcgatttttt catccacttc ctttgccttc gcacgcgcgg
     1921 tatccgccgt tacggacaaa aacgcgtcaa ccttcggctc taccgcgtct atgcgcgcga
     1981 gcgtgtcgtc caggatctcg cacgcggtca cttctttgct gcgcagcagg gcggaaagct
     2041 ctgccgctga ttttttatac agttccattg tatgccctcc tcactctacg gtcttcggca
     2101 cgacgatgca gcccgcaacg gcttggggca cgttttgcag catttcatcc cgcggatacg
     2161 agggcacaac ggcatcctcg cgcagctcca tgacattgcc cggctccagc agcgtggccg
     2221 cactggggat ctccggcaga ttctccacca tcgcaacgat accggaaagc tctttttcaa
     2281 acgcttccac ctgttcctcc gggatcttca gcatcgcaag cttcgcgatg tgccgtacat
     2341 cgatttccat attccattcc tcacttttat cagaattgtt cgggcgcccc gcacagcgcg
     2401 gcgcttaagg tgtcagcttt caatcgtatc gcgctgcgct tggagcatcg caagaaattc
     2461 ttcctcgctg ataacaggga cgcccagcga ctgcgctttt gtcagcttgg agcccgccgc
     2521 ctcgcccgcg agcacatagc ttgttttctt agagacggac gacgacgcct tgccgccgtt
     2581 ctgtgtgatg agcgcttccg cctgcgtgcg ggaaagcgtg ggcagcgtgc cggtaaccac
     2641 cagcgttttg cccgccagac ggtctgtctt tttcggcccg tggtagcgca tattgacgcc
     2701 agcctcccgc agacggcgca ccagatcctg cgtaccatct ttggcaaaaa agtccagcac
     2761 gc
//
LOCUS       DAWBSB010000268         2743 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_5888_length_2743_cov_2.170015, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000268 DAWBSB010000000
VERSION     DAWBSB010000268.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5888_length_2743_cov_2.170015"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          588 a          827 c          699 g          629 t
ORIGIN      
        1 ctttgctgtc ccgaccgccg gtcacggaca ctgccggtcg ttcccatttc tgcgcgatca
       61 gtccgagatt gcttttcatg gtctccgcaa tctggtgtac cgttttgcga tactcctcct
      121 cgtctgcaca catatggaga gacttgcgcg gccaaatacg aacggttttg aatttaccgt
      181 ctgaataaat tacatagttg ttgcaaatca gctttttcag ctcgtgatag tggctgatat
      241 ctcccggcag tccttccccg tacagatgat accagcggta atgttcaagc ttggtcacat
      301 agtcatcacg gtgaagttgc tcaaacagtg aaaccaattc ttcgtgagag caaagataaa
      361 aatgtccgtc aatgtttcca tagtaagcgg tacgcatact ctcaaagtct ccgcagaacg
      421 tcatgcgctc cccggcaatc attccaagaa caaatagtcc cgtcaaattg ttaatggtct
      481 gaagaccgag ttcaaagctg ccctgagact gctccgcaag ggtacgcaga atgtctattt
      541 catccgcctg atttgaaaag ggattgtatg cgtggccaat cagaaagtac gtaattccat
      601 tcttttctgt ggtgtatagc ttttgccgac agtgaagcca gatatgaaag ctgtccgaaa
      661 ggctctcctt ccgccagttt ccaaagaagg gaaacgcgcc gccaattgtg agattctgat
      721 tcgtaatcag gaaaccctgc tcaaagatgg aggacttata ttccggaaaa cgcttgatat
      781 tttcttttat atacggccag ttttcattca tcccaagttc tcctttatcg tctcccacag
      841 ccttgcccga tttccctctc ccacaaagct gttatgtggg gtcaggagca cgttttccat
      901 atcccacagc gggctgctct ccggcagggg ctcctccgca aacacgtcca gcactgcgca
      961 gccgccgttt tgcagccact tggtcagcgc agcctcgtct gccagcgcgc cgcgggcgac
     1021 attgacgaag atcgcgccat catgcaggcg gccaaagatt tcctcgctca cgatgccgcg
     1081 cgtctccggc gtcagggcga tgcagcacac cagaatatca caggccgccg cagcgtccgc
     1141 cagcttatcc agcggcagga gttcatcaaa atccggcaac tcccgcacgg tgcgattcac
     1201 gccggtgata tggcagccaa aggctttcag ccgcttggcg atctcacgtc ccacatcgcc
     1261 gcagccgagg atgcacacgc gcttgccgga aagctccagc aggccgcgat gcttctccca
     1321 tttgtgctgc gcctgattcg ccgcaaaaaa gcggctctgc ttatatagct gcaaaatgcc
     1381 gcagacggcg aactccgcca tcggaacgct gtacacgccc gcagcgttgt gcagcgcgat
     1441 gccgcgggcc cagatatcgt ccagcggcac acggtcaagc cccgcgctgg tgagctggat
     1501 gagtcgcagg ctcgtaaagc gctcgatgga attgtagagg aacaggccgt tgcacacgac
     1561 cgcctcgtat cgctccgggc tgtctaccgg ggcgcgttcg tcaccatgaa cgaagacctt
     1621 gtgcccggca gcttccagct gctcgcgctc gccgctattg agttgaaacg cgccggtcac
     1681 caaaacgttc atgcgttcat acctctcaca atgttctcaa cgcaggagca aaccctgcgg
     1741 tgattctcgt caaactgctc ctgcgtccaa gcggcgcagt cccgctcaac gcggcggata
     1801 tccgccactc cctcggtaaa aatgcgccgt atttcatcca agtccgtcgt cttctccgtg
     1861 ttgcagaagc tgcgggacag gattacatac tgtgaaccca gccgatagtg ctctccgata
     1921 atatattctg ctggaagcgt ccccgagccg gggcggccca cgccgccaaa gccataggga
     1981 atacttgctt cacggagctc ggcgcaaagc ctgtccaccg tgccgtccgt caggagctgg
     2041 aacatgaatc tctgatgata gcacaggtgc aggtcattga ggccgatgtg catctggtcg
     2101 atgcccagta tggccagcgc ctccggcaga ttgtctgccg ccgtgtcggt ttccagcagc
     2161 ggcatgacct ttgcacggcc atccaccatg ctaacaagct gccgcagatc atccgccgtg
     2221 cgccacatgg ggagcataat aacgtccgca ccgtttgcga tggcgtcgtc gatctccgtc
     2281 tgcgagttgg gattcagtgg attgacgcgc gccatgatct cggcctgatc gccgatggcg
     2341 gcacggactt ttgcaatatc ctccggtgta tggtgtgact tcaccgtatc catcccgccc
     2401 tgacgctctg ccttccccag cacctccatg tcgatgaaga tgcggtcaac gcctgcatcc
     2461 gccgcgattt tcgcaaccgc aggatcattt gtgatataca tgagtttcag catttgatct
     2521 ctctcagacc gtcgcgccgg tattgacatt atcctcgttg cgcaggacct tgaacacggt
     2581 catgaaaagg atcttgatat ccagcgcaag ggagacgtgc tgcgtatacc agacgttcat
     2641 cttgatacgc tcgtgccact ctacgccctt gcggccatgt acctgcgccc agccggtgat
     2701 gccggggcga acggcaaaca tatgaaactg ttcttccgtg tac
//
LOCUS       DAWBSB010000269         2719 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_5946_length_2719_cov_3.658784, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000269 DAWBSB010000000
VERSION     DAWBSB010000269.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5946_length_2719_cov_3.658784"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          489 a          919 c          715 g          596 t
ORIGIN      
        1 gatacggctc gaatactttg tccgcgggaa aatattttgc ctggtacagc tgtttgcgca
       61 aagcatcctc cacaatacgg gcgtccgcat ccctgcaccg gagcttcaag tccccgtcca
      121 catccagctc atgccagggc aactggtcca tcatgatcag agggcggcat tcgcgcagcc
      181 cgttcaatgc cttccacaga gcgcgccgct cttcatttac gggcgcaaac gcgacctccc
      241 ggtattcttt tgccagttgt gtaagaattt gttgatccgc attcatgctt tccgctcctt
      301 tcgccgtgcc cttcggcacg ggcttctttc acaggacatc cttgcttccc cgtctgtctc
      361 tgcctcaaag ctccagcttc atcagtcgtg caattgcaag aatatagatg cgcagcgctt
      421 tgtacagttg cgccatttcc atggcctcgt ccgcaccgtg gggggcgccc gcccaaacag
      481 ggcggggctg gtccgtctcg ccgggaccgt agctgatcgc atttgcaaac cggcgcgcat
      541 aggttccgcc gcccatcgca aaaggcgcgt cgctgcggtt cgccagctcg ttataggtat
      601 ttgtcagcgt gcggatctcc ggagaatttg ggtcgatgca gaacggagcg cgcatggcgc
      661 ccggctcaaa gctgccgcct gcgttttccg ccaacgcgca caacgccgcg cgtagccttt
      721 cgccgtccat ggcggccggg taacggatat ctacgctttg acgcaggcgg ccctgctcaa
      781 gacgtaccat cccgcccaca catgtgagag ggccgaatac atcgtcgcgg gccgctacgc
      841 ccagcgcact gccgtctgta tccgcgtgca gcgtgcgcag catccgaagt gcccgctcct
      901 cctgcggcgt gcacagaccg ttgtccagca aataacccac caccagcccg atggcgttga
      961 cagacccctg cggcccggcc acatgcccgc cttttccccg cgcttcaatt cgcacacagc
     1021 cgttttcagc ccgtattgtt atgttttccg tatccggcag gcgggtcttt tctgtccgca
     1081 ccagcgcata ggcgcggtcc ggcaccacgt tttttgcctc gccgccaaaa aattccctca
     1141 agcagccgtt cagcggcgcg cttaccagcc agccgctgta tccccccttt tccgcatatc
     1201 ccaccgggaa acgcgcgtcg ggagaaaagc aaaatgcggg ctgcggataa tgggcaaggt
     1261 agtagtctac atcctgcatg cccgtttcct catttgtgcc gaacaaaacg tgcagcgtat
     1321 agggcagttt cacgccttgt tccttaaaaa attttgcggc atacagtgtg atgacggccg
     1381 gccccttatc gtcggctgtg ccgcgcccca ccgcgaatcc gtcccgcagc gtcatgcaga
     1441 acgggtcgcc tgtccatccg ctgccctcgg gtaccacgtc cagatgtgtg atgagcgcga
     1501 tctgtttttc gcttttgccc ggcaagtcgg cataccccgc gtagccctcg cagttccgcg
     1561 tggaaaggcc catacccgaa gcaatttcca gagcggcctc cagcgcgcgc ttcggccccg
     1621 tgccgaaagg cgcgcccggc gctggctctc cctgtacgct tttaatgtcc accagcgttt
     1681 tcatatctcg caggatgccc tcatggctgc gcttcacaaa tgcgtcgatc tgccggagca
     1741 gccgtttttc attttcgtcc ataccggttc tccttttttg cgcgctgctc agcgcacagg
     1801 cagttcccgt gcaaacagag cgcacatgtc ctgcgcgtcc tccatacagg cggcgtcata
     1861 ccacaccact gttttctggc tcttcagcag gtccatgttc gactcgatct cggctcgtgt
     1921 cagacgcagc accgggatca cccgggtcag tttctcccgg ctgcgcttcc acacctccac
     1981 cgcacggtcg gtgttcgggt ccgaggaaag ctccatcagc cggatattcg gcaacgatgc
     2041 gacagaggcc aggcatcgtt cgctgagggc atgcatgtgg ataaacacct cgtcgaattt
     2101 gcggaaaatg cgctccgtgc gcggccggcc gaactcctcg aactgctcca gagagatcat
     2161 tgtggtcgta tcctcggaca catgccccag gccgcggccg ggcagccatt cgccaaagcc
     2221 cgtcaccacg ccgccgtata cgtctcctgc cgcctccatc tgtttttcat agttccatac
     2281 aagcgcgtcc gcgcagtaat ccagcagcgc cttcaggtcc tccggctctt catagaaatc
     2341 gtagaaaaag tcgttgccgc gcagcgtgtt tgccatctcc agcgcgccgg acgcgccgcg
     2401 caccatgggc agaaaataat cgctgtactt ctcgcgcaga tatgcgatac cgccgttcac
     2461 catccggaat accgggttgt tttcatccat tgccagcttt tccagttctc cgggcgcatc
     2521 cagcatcgcg taatgccagc tcgtatcgct ctcgtagtcc acccggccgc ccagaaaggc
     2581 cgtgtgttcg gcaatgccgt accacggccc ttccgcgggc accgtatcat ccgcaagctc
     2641 cgcccgctgc gccagacgtt cgatggtaca ctgggcgcgg gcgtccagat atttgtacac
     2701 atcttccgga aatgtgtag
//
LOCUS       DAWBSB010000270         2716 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_5960_length_2716_cov_2.491920, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000270 DAWBSB010000000
VERSION     DAWBSB010000270.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5960_length_2716_cov_2.491920"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          560 a          772 c          903 g          481 t
ORIGIN      
        1 gccctacatc gccatcgtca tcgatgagct ggccgacatg atgatggtgg ccggcaagga
       61 agtggaggac tacatctgcc gcattgccca gaaagcgcgt gcggcgggca tgcaccttat
      121 cgtggccacg cagcgcccct cggtggacgt catcacgggc cttatcaagg ccaatatccc
      181 cagccgcatc gctttcgccg tcagcagcca gatcgacagc cgcaccattc tggatgcggg
      241 cggcgcggaa aagctgctgg gcatgggcga catgctgttc atgcctgtcg gcgcaagcaa
      301 gcccgtccgc atccagggga cttatgtgcg cgacgaagag atcagcgacg tgttgaactt
      361 catcaaaaag cactcttccg ctgattacag cgaggagatg atccaggaga tggacaagcg
      421 cgcggtacct gaaaagggcg cgggcgacag cggggacgag gacgacagcc aggatccgat
      481 gctcaaaaca gccgtggagg tcgttatcga cgcgggacag gcgtctacaa gtcttttgca
      541 gcggcgctgc aagctgggct acgcccgggc ggcccgcatc atggacgaga tggaacagct
      601 gcacatcatc ggcccgtacg aaggctcgaa gccgcggcag gtgctcatca cccgccagca
      661 gtggatcgag atgacgatgc agcaggcgtc ggattcgacg ccggaggtct gacaggagat
      721 accggatgca gggcggactg gtccgccctg catttgcggt tttgtccgcc gggattttgg
      781 aaagcacagt gaatacggaa acggaaaagg atcgttttat gagtgagaat acaaaaaatc
      841 cggccgtgga gaagtttctg cctgtctgca aagccgatat gcttgccagg ggatggcggg
      901 acgtggattt cgtgctggta acgggggacg cctatgtgga ccaccccagc ttcggcgcgg
      961 ccattatttc gcgtctgctg gaggccgagg gttaccgcgt gggcgttctt tcccagccgg
     1021 atttcaccac ctgcgaaagc atgaaggagt tcggccgtcc ccgctacggg tttttcatcg
     1081 gcggcggcaa tgtggacagt atggtggcgc actattcggt ggcaaagatc cgccgcgccc
     1141 aggacgaata cacaccgggc ggtgcggcag gcaagcggcc ggaccgcagc gccaccgttt
     1201 acacgcagct ggcaaagcag gcttacccgg atcttccggt cattctggga gggttggagg
     1261 cgtcgcttcg ccgctttgcc cactacgact attggctgga tgatgtgctg cccagcatcg
     1321 tcgagagcag cggggcggat atcgtttcct tcggcatggg ggaacgccag acggcggaga
     1381 ttgcacggag actggcggcc ggggagccgg tggaggagat caccggcgtt gcggggacct
     1441 gttatctgac ggatttcgcc ggtctgcccg cgtcttatgt ggagtgcgcc tcttttgcca
     1501 aagtgcgcgc ggacaagacg gcatatgcca gagcctgccg catccagatg gacaaccagg
     1561 acgttgtctc gggcaagccc gtggtgcaaa aacaatcgga gcggtatctg gtgcagaaca
     1621 tcccggcggc gccgctcacc cggcgggagc tggacgccgt gtatgcgctt ccctttacgc
     1681 ggcgctatca tccctcctac gaagcgctgg gcggcgtgcc tgccattgaa gaggtggaat
     1741 tttccatcat ccataaccgc ggctgcttcg gcggctgtaa tttctgcgcc atccagctcc
     1801 atcaggggcg cagggtcacc tcgcgcagcg cggacagcgt ggtgcgcgag gccgaggcca
     1861 tgacgcatgc gccgggtttc aaggggtaca ttcatgacgt gggcggcccc actgcaaact
     1921 tccgcctgcc cagctgcaaa gagcagatga aaaacgggat gtgcgcgggc ggcaagcact
     1981 gcctggcgcc gcagccctgc ggcaagatca tcgtggacca cagggaatat ctgggcatcc
     2041 tgcgcaggct gcgcgcactg ccgggtgtga aaaaggtgtt catccgcagc ggcatccgct
     2101 atgattacct cattcgcgat aaagacgaga gctttttcaa agagctggtg gagcatcatg
     2161 tgagcgggca gctgaaggtg gcgcccgagc actgcgcgcc ggatacgctg cgttatatgg
     2221 gaaagccgcc catcgaggtg tacaacaaat tttccaagcg gttctatgag ctgacgaagc
     2281 aggcgggaaa gaaacaatat ctggtgccct acctgatgag cagccatccg ggaagtacac
     2341 tgcgggacgc ggtgacgctg gctgaatatc tgtataaaaa caacatcagg cccgagcagg
     2401 tgcaggattt ttatcccacg ccgggcacgg tgtccacctg catgttctac accgggctgg
     2461 acccgtacac gctcaagccg gtgtatgtgg cgaaaacgcc ggaggaaaaa gccatgcagc
     2521 gcgcgctgct gcaatattac gagccgaaga acacgccgcg ggtgcgcaag gcgctgcagc
     2581 tggctcatcg ggaggacctg atcccgcttt tgtgcggcgg gcgcggggct gggaaaagcg
     2641 cgggcacacg gccgtgcagc gcggcgcgcc cgcagcctgc gggcggtgcg cagcggcatg
     2701 gcggtggcac aaacgg
//
LOCUS       DAWBSB010000271         2704 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_6000_length_2704_cov_2.197810, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000271 DAWBSB010000000
VERSION     DAWBSB010000271.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6000_length_2704_cov_2.197810"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    862..871
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1646..1655
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          616 a          800 c          729 g          539 t
ORIGIN      
        1 ccctttgaca gcgtgcgccg cgcctgcagc ttcaaaaagg ccgcgcccat cacgaaactg
       61 aacacctcca tcatcaccgc cgccgagcag cagaccatga actattatat gaacatcgcc
      121 gcgttttaca aaagcgaccg cggccggaag ctctaccagg agatcggcat gatcgaagag
      181 cagcacgtga cgcagtacgg cgcgctgctg gatacgaagt gcacctggct ggaaagcctg
      241 ctgatgcccg aatataccga atgctacctg tactggtcgt gcttcaacga cgagacggac
      301 cgtcctgtga aaaaaatctg ggaacagcat ttccatcagg agctttccca cctgcacgcc
      361 gccgcgcgcc tgctgcagac ctatgaaaag aaagagtggc ggcaggtcat tccggacggc
      421 gaattccccg agctgctgaa attcggcccg cagaaggaat atatccgcga tgttctggcc
      481 ggcacagtgg aatggacggc ggacggcgag gagttcaccg acgtgcgcac gctgcccgca
      541 gacttccgct tcttcaatta ccagcgcact gtaaacgcac gcaccgcgca ggttcccagc
      601 cacgctgtca tagaggatta tctggccgaa tacggccgcg attaccgcta tgaggacgca
      661 ccgcatcctg tgaaggcgct gcaggaccgc gagaaggata acacccgcgt gggtcgtacc
      721 cgcagctgca caaaataacg ctcgcccgca aaaaagcgtt tctcagcctg aatcaaacgg
      781 accgcaccaa taaaaaaacg gacaacagac aaaaagcccc ctgatgcaga aaaccaaaac
      841 atcagggggc tttctatgga cnnnnnnnnn ngacaaaagg aattgcatgc ataggcagga
      901 attattgcca aaaattaaag acaggaataa attgcggaaa ttccggaaga aaacagctaa
      961 atgtacagga ctctggaaat gcatatgcgg taaaggcccg cgcaggccgt caacgataca
     1021 aacgaaagaa aaccatcacg ctcacgcgtc tacacaatgg cagaaagcct tccctgtttt
     1081 gagagcaaag cgcattttcg tacaaagcct ccactgcctt catatgaccg gcctatgttt
     1141 atttttacaa ccgggaatgt ctttaataca aaacaggagc agcgccgccg cgccgctcct
     1201 gccaaaacct gggagttttt tcacagggga tccttttaat actgtctgca caatcgggga
     1261 cggttcaccc gcgggaggga acttgtgtgt taccgaaaaa gtaaaattac agccgggcgt
     1321 cgaacaggcc gcagaagctg tcgatcgggt cccgcccctt gaacgcgtcc ggccccgcca
     1381 taaggtcgtc caccagcgcg tcgaaagtgg ccggctttgc atcgtaggtg ttgatgtagg
     1441 tgcgggcctg gggcacgtcc gccagcatgg tgggctgccc gcagccgatg acgaccaccg
     1501 gcagctcgaa tacataccag gggatctctc cgccgccctt ggacaggcca aagctgggcc
     1561 gcccgctgga aacgtcgcac agcgtgatga caaggtccat gccggaaacg aagtcgtcga
     1621 tggcgttctt gcccgcgaaa tacagnnnnn nnnnntcatc gcgtccagcg ggctttcgta
     1681 gataaacgcg tcaaagcctt tttcacacag gcgctcctta agcatctcgg ccgggttttt
     1741 accgccgccc atgcccatgg ctttcatcag catgcccatg ccgctttcgg cgcccttgac
     1801 atgcacgatc ataatacgtt tataacgctc cggtgtgatg ggcagcacat ccctgtcctt
     1861 gtattttacc agtgtgatgc agtcccggct gatggccttg tgcatctcct tgtattcctc
     1921 tttgcccagc gccccggcca gcacttcggg cgcaggcaca agcgcttcct tcgctttttt
     1981 gttcaggccc agatgcgctt tcaggcccag gatacgcgtg agcgcttccg tcatgcgggc
     2041 ctcgctgatg acgccgcttt tgtatgcatc cagcatggta ttgaagtcct cgtccgggtc
     2101 gttgaagaac aggaacatat cgcatccggc attgatggat gccggcagca tctcgcggcg
     2161 ggtcatgcgg tccgtcatgc ccaccatatg ggaggcatcc gttaccacca tgccgttgaa
     2221 gcccagcttg ccgcgcagca gcgtggtcat gatctccggg ctcagcgtgg cgggcatcat
     2281 gtcctcgtcc ttcagcgcgg gattgatggc ccgcgcgtac tccggcagca tgatatgccc
     2341 gcccatgatg gcatccagac cgccgtcaat aagcgtttta tacaccatcc cgtaggtctt
     2401 gtcccactct tccacgctga aggtgttgac attgttggac agatgcgcgt cgcggaaatc
     2461 caggccgttg cccgggaaat gcttcgccgt acaggcaaag cccggaatgg tgtgcaggcc
     2521 gtccatatac gccttgctca tctgcgccac gcggacgggg tcgttgccga aagcgcgggt
     2581 cacgatctcg gtattctccc agttgtagtg gatgtcgcac accggcgcga aagccatgtt
     2641 gcagccgatg gccgcagcct cctcgttggc cattttgcca agcgcatagg cgtaatcgtt
     2701 gttg
//
LOCUS       DAWBSB010000272         2671 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_6077_length_2671_cov_2.979358, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000272 DAWBSB010000000
VERSION     DAWBSB010000272.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6077_length_2671_cov_2.979358"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          541 a          822 c          729 g          579 t
ORIGIN      
        1 gccatccaga aagccatgga ccatctgatg gagggccgca ccagctttat catcgcccac
       61 cgcctgtcca ccatccgcga cgcggacctc atccttgtga tgaaggacgg agatattgta
      121 gagacaggca aacacgaaga gctgcttgcc aagggaggct tctatgcgga gctgtaccaa
      181 agccagttcg cggatgtgaa cgacgaggcg gtgtaacatc gtccgaccgt ttccccttcg
      241 cgcgtcccgt cgggggacca ctgccgccgc agaactcttt tctgctcaaa acaataacaa
      301 aacagcgcgt gcttgccggt gccaggcaag cacgcgctgt tttgttgaat ggtccggccc
      361 tgtccgcggc actgccgtgc tacacgaagc gcacgtcgat gcggccgttg ctggtggtgg
      421 cccacagacg cttggcctgg tcctcatagg cacaatccat gggcaggttg ttctgcgcat
      481 tgctggtgcg gctgctgatg gcgtaatccc gcatatcccc cacgaccgtt cccgtaatgg
      541 cggcgttggc tgttttgaac gtgaggtccg gcgcctccac atcctcggcg cggacaggcc
      601 cgttgctcgt ctgcatccac agcttttcgg caaagcggca gtgctcggct gtgatacgcg
      661 cattgttgga ggctgcctcg cagtctccgc ctgtcacgcc ggacagcgtg atgggcccgt
      721 tcgaggtctt ggcagcaagc gagccgaaca cgcagttttc cacagtgatt cgggcattgc
      781 tggttacaaa ccggcccttc cctccggctg aaaggcccga gaccgtgata ccggcgttgc
      841 atgttttgac gcatacctcg ccggcaaagt ccagcggcag ctccagtgtg atgtcatgcg
      901 gcccgcgcag caggtccagc cagttccata cattaaaaat cgacatggta tgggtaaaca
      961 ggaatgcgcc gtccttttcg gccacagtga cacggtcgtt ttccctcggc tgaaacaaca
     1021 cccgcacgcc gccgcccggc accgcgcgca cgttcacacg cacgttgcgc gcgtccacca
     1081 ctacggtatg caccggctcc tgagcgggat actccagaca tgccggccga ggcggtaccg
     1141 cgccctgccc gggcgccccc tcaacagcct gctggctctt gtattcggca cgaatctgcg
     1201 ccgcgatctc ctccggcgcg ccgaagcctg cgatgatttc ctcctcgggc ttaccattct
     1261 ctacgccgtc gaaaatcagc tcgttgtaat aatccgccac ctgacgctgc tccaccgggc
     1321 caagctgcgt cagtcccgcc atcagctggt tgagaaacgt ttctctgttc atacctcttc
     1381 ccccttgata aatgcataaa tgcgcttcac ttcgtcccat tcgtccagaa atgcatcaat
     1441 gcgttcctgg cccgcgtccg tgatgtaaaa atacctgcgg atacggccgc cgtgctcctg
     1501 tgtgtccgtg gtgacgtatc cgtttgcctc cagacgcttc aggatgggat acagcgtgga
     1561 ttccgacacc tcgatgcagg aggatacctc cccgatgatc ttgtagccat aagatggtcc
     1621 ccggcgcagc accgccagca cgcatacgtc cagcaggccc tttttccgtt ggatatccat
     1681 tccgtgcgct cctttcctga tactatgcaa tacatactat gtataatata gtatgtacca
     1741 ggtgtcttgt caatactccg gaaaaatttt tatcgcacgc acaaaacgta aaaagggcgt
     1801 ccggctgtgc cggacgctcc cgcgaagcgg cttcagcaaa accgcattga atgcggtttt
     1861 gcatggttca aggttcccaa agccttgtgc gccagcacag gggcgtgtgc catgtacacg
     1921 cgcagccgcg caagcggtgg ctttgaagtt attgtcctat ctcaaaatca cattgtacgg
     1981 atgtatctcg ttcaaacgca ttgcagttct tgcgctgttt attccaacag atgccggagg
     2041 tgctctccaa aagtagacat gctctgcctg ccatgctaca ctggatacac cgattgcaaa
     2101 aggaggatat tcgcatgaca tggaacacgc tttggcctca gaaccgaaag ccctcacccg
     2161 atgatatcgc agagtttgtg gacaatcccc tgtggaaagc actgtgcagc tggctggaat
     2221 cgggctaccg cacggtgccg cgcatcgaat acagccgctg cgggatggcg ccgggctgga
     2281 atgtaaaata caaaaaggga ggccgcgcac tgtgtacctt gtacccggcg gccggctcgt
     2341 tcacctgcct tgtgtgcatc ggccagcggg aagccatggc ggcagaggcg ctgcttcccc
     2401 tctgctctcc tccggtgcgg cagctgtacc acacggccag cccgctcaac ggcagccgct
     2461 ggctgatgat accggtaacc gatgaagctg tgcttacgga cgtgaaagag ctgatcggca
     2521 tacgggcggc ggcttaaagc caaagcggcc cgcccctgtt ctttatggcc ccctggctgc
     2581 ttttccgggc cgtgagaagt ttttattttc gaagcgcagt gaattgtcaa actaagtgca
     2641 acaggaattg agctcgaagt cccataattc c
//
LOCUS       DAWBSB010000273         2658 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_6113_length_2658_cov_2.371879, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000273 DAWBSB010000000
VERSION     DAWBSB010000273.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6113_length_2658_cov_2.371879"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          569 a          828 c          669 g          592 t
ORIGIN      
        1 ggttaaaatt gaatcatgcc tttaggttgt tggacgctgc tatggatttg acagaccaac
       61 gtggaaaaag cgcggcacaa tgcgctttta tacgcacttt gaaagggtta taacgacgta
      121 ttttaacctt gtttgccctt tcaaaagtac gacttaaaat ccctcggtgg caacaccata
      181 cgggttcgat tcccgtcagc agcaccataa agcaaagcga aaggcatgcc gcacgggcat
      241 gcctttcgcc gtcagaaggg aaaacccaat atgcgcgaat ttgtcatcac cgatgcttcc
      301 atgccccgtt tggactccgc gctgcttgcg cagtatcctc agctttcccc cggcaggctg
      361 cacaagtatc tgcgtgaaaa caaaatcaag gtggatggaa aaaagcttcc gctctctgca
      421 cgcctgccac gcggcagcac cgtgcgtgtt tttttgccgg atctgcttct ggataagccg
      481 gacggcccgt tatttctttc cgcaaggccg gagctgtgcg ccgtttatga agacagcgac
      541 gttctggttg ccgacaagcc cgccggactt cctgtaacgg atgaagacgg acgcactgcc
      601 gacaccctca tcaaccgcgc tctcctgtac ctgtatcaca aaggcgaata tgcgccggaa
      661 cgcggctgga ctccgcgcct ctgccatcgg ctcgataccg gcacgtccgg tcttgtgctc
      721 attgccaaga cgcagcaggc tgaagcgctt ctgctgcacc ttgtaaaaga gcgtatgatc
      781 gaaaaaaagt acctttgcgt cacctttgga cgcccctcgc ctgcggcagc aacactgcgc
      841 ggctatctgc aaaaggacgc ccgacaggcg cgtgtgcgta ttctggacac atccgcgccc
      901 ggagcaaaag atattgtgac aaaatataaa acgctggctg tcagcggacg gctggctttg
      961 ttggaggttg aactcgtcac ggggcgcaca catcagatac gcgcgcattt ggcctccatc
     1021 ggctgcccta tccttggaga cggcaaatac gggaacaacg cggccaaccg ggaactgaag
     1081 ctcaaatatc aggctctgtg cgcgtacagc cttcactttc ccgcgttgcc ggacggatcg
     1141 tgcgcggcgc tctccggcaa aacgctgtat gcttcccggc cctggtatta tgcgcaggtc
     1201 ctggacggaa cgcttaaata aagcgttccg tatcgccatc tccgctgttc acgccccatg
     1261 ttttaaaaaa aacaaaagcc gctccgtccg caacgcacag aagcaacttg cggacagggc
     1321 ggctttttgc acagcttata tcaacgaagc actgtttccc ggcacggtct gtacgaattc
     1381 ctcataagtg tacagactgt tgagcacctc gcacagcgct tttttggaaa ggcgcggcgg
     1441 caggccaagg ctttgcagcc aggcgcgccg cacgcccgcg cttccctctg tgccggaaag
     1501 acccagctcg tacaggtcgg tataggtgat ggcgcgccct gcgcgcttcg ccttttcccg
     1561 cgcgcccgcg gtctgcagtg cgcgcagtat cgcttcggac ggcacaccct ccacgcccag
     1621 cacgccttct ttcgaaggct gagctttacg ccgctctttc cctgcgatgg ccggaacata
     1681 tgcatttttc acacatatgc cctgcgccag cttattgata taattgcgga tgcggaaccc
     1741 ggcggcatcg gaatcggtga aaacgataat gccccgcttt ttgcccagtt ctacaagcag
     1801 acgctttgtc tccgcatttt taaagaccga aaatccattc gtcgtaagaa tgagcccatc
     1861 caccaaatcc gccagcttcg ccgcgtcgta cttgccttct acgacgatca cttcttccac
     1921 gcgcagcttc ttcatcgccg ttctccgatc tgcatcactt cgcaaagagc cgtctgcagc
     1981 agcaccgttc cgtctgcggc agatgatttc aacgcggcat ccagccccag cagcacactg
     2041 aggatgtgct ccagctgggc gcgggaataa tgggacgccg tctcaccgga tttgcgcagg
     2101 cggtaatcgc ttccccgata tgaaaaatcc ttgtgcacgg ccgcataatt acgatgtgcc
     2161 gctgcgccgc acttcacacg gtacatatct acaaacgagc cggacagcgc cgccgcaatg
     2221 gcaatgggct cattttgcag ttccaacagc tgagacagct tggccatcgc ccgtgtttta
     2281 ttgcgcgccg tgacaagacg caccatttca aacacatccg cttcgatgct ctgggtgccc
     2341 atttgtgcga tcaattcagg cgtgatctct ccataatcag ccacagccgc cagcttgctc
     2401 agctcgctct ccaacgcaaa caggtctgtc ccacagcgtt ccaccagctc agacgcagcg
     2461 cccggggaca aaaccgctcc cagctcccgg gctttttcca cagcaaaccg ctttgcgtcc
     2521 tgaggcccgg gctttacaag ttctgccgtc agcccggcct tctccgctgc gccgatcagc
     2581 tgtttggcct ttttggagag cttcgccttg tcgtctttaa acactgtgct catcacaagc
     2641 actgcgtttt ccagactt
//
LOCUS       DAWBSB010000274         2652 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_6131_length_2652_cov_2.534848, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000274 DAWBSB010000000
VERSION     DAWBSB010000274.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6131_length_2652_cov_2.534848"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    887..896
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          570 a          833 c          733 g          506 t
ORIGIN      
        1 cggctgctcc ttcggggcac tttccggctg cggcggcgtt ttctttttct tccggtccac
       61 cttctccttc atgggataca ggtggaagct gaaaatgccc aacagtttca gtctggccgt
      121 aaactctccc tgcttatact ccaccgctac agccgccgga acaataagca ggaccgcaag
      181 cagcgccaaa agccccagaa ccacccaaag cagcaccatc agaaacgtgt gcatcacaca
      241 tccccttccg gtgcaaacgc ctgtgcgccg tccgcgtcct catcgccgga atacgcagcc
      301 tcccccgcgg cctcttccat ttccgcatag tcgtcaccgt gcagcggcgg cagctcagcc
      361 agcgaggcga tgccgaacgt acgcaggaat acatccgtcg tgcggaaaga caccggccgt
      421 ccgggcaggt ccagccgtcc ggcttcctcg atcagccctt tctgcaccag cgtctgcact
      481 gtggacgaag aatccacgcc ccgcacctgc tcgataaagg agcgggacac cggctgattg
      541 tacgcaataa tggccaggac ttccagcgca gccggagaaa gcggcgtgtt gcgccgcgtg
      601 tccatgatat ctttgatata cggcgcataa gccgttttgg tggcaagctg ccagcggtcc
      661 tccagctgga gcagcacaag accgcggtct tcggcctcgt aatcgtcccg caaggaaaga
      721 agaaggcgcg ataccgcgtc ctcctccacc cccagcacgc ccgccaggcg ttccgccgtg
      781 acaggctcgg catgagcgaa cagcatggcc tctaccgcgg ccttggtttg tttcagatcc
      841 attttaatcc tctcccgctt ctctggcagt cttaaggggg cgtatgnnnn nnnnnngggc
      901 gtatgccgtt acattttacg cccggtgcgt acggtgagcg attcggcgtc cgcgatggcg
      961 atgcgtccgg cgcgcaccag ttccagaacc gcgaggaacg tagccacggt ctggctgcgc
     1021 cccccctcct tgacgaacag ctggcgaagc cgcctgacac ggcctgtcat caagccgcgc
     1081 agcacatgta tgatacgtga ggtgacggaa acgaaaggcg cggtggtaag cggttcgaac
     1141 tgctcctgac ggggcggctg cttctgacgg ctgcgtccct gcagggcgtt gtacgcttcc
     1201 tccagcagat atacatcgtg acgcaggcgg tactcggtat ccagctcgac ctcggccggt
     1261 tcacgcacca cggtatatac gccttcggcc atgctgcgca gctgcgcagc cacctcctta
     1321 caggcgctgt attccaccag cataccggtg agttcttcct tcatgcgctc ggcttcttcg
     1381 ctcttgggca gcaaagaata cgacttcatc tgcaccagac gggcggccat ctcgataaat
     1441 tcgcttgcac tctccatctg gtaaacaggc aggccgttca ccatcgaaac gtactggtcg
     1501 atcagggcca caatctcgat gtcataaata ttcattttgt ttttggagac gaggtacagc
     1561 agcaggtcca gcggcccctc aaaatcttcc agcttaaaag taatcgtctg ttccatccgg
     1621 cagggtgatt ctccttttca aagcgctgtt caaagcacgg cgcgcagaat ggcctcgata
     1681 aatcccgtgg ccgacaccag caggttccac acaaaatttg tggcaacgcg cagaggcgtg
     1741 tccagccagc ccagcatcaa aaccgcgaac agccccacca tgatataccg ttcatagcgc
     1801 atcagcccga aataggcccc gcgcggcagg aacaccgttg caatgcgtga gccgtccagg
     1861 ggcggcacag gcatcaggtt aaacacggcc agcgtgatat tcgtccacac catctggaac
     1921 agcaccgcgg atacgaatat ccagccctgc gtgccgggtg cgaggtagta caccagcttg
     1981 taaaagatca tacacaaaaa ggccagcagc agattcgatg ccgggcctgc ggcagcggag
     2041 agcgccatat ccctgcgggg atgccggaaa ttcgttgccg ccgcgatagg cacgggcttg
     2101 gcccagccga agccgacaaa gatcatgcaa agcgcaccca gaaaatcaaa atggcgcagc
     2161 gggttcagcg acaaacggcc atagcgcttg gctgtagggt ctcccagctt gtccgacgcc
     2221 cacgcatggg cggattcatg cacggggatc gcggtgagca gcgcgacagc gcgggccaca
     2281 taggtggcaa gcaggtccca attcacgcga tgtctctccc cttccggccg cggcgcacag
     2341 caggcgcgtt ctgcggcgta aactttatcc aacggatatt atatgatacc atttttccgg
     2401 cacaaacaca agtgcgcgct ttcacttgcg ggacggcgct cggacagtat ttgcgagcag
     2461 cacttacggg cggcttctcg ccgatgagcg tgttgcacaa agcgcggcaa tatgataaga
     2521 tggcagtaat cccactttct gcgcggctgc agcctgcaca tcggtgtgcg ccgcccccgg
     2581 tggaagcaaa cagaaagtga ggcgttgtac gggtgcaaat tgcagtatcg gcaagccccc
     2641 gggcatttat aa
//
LOCUS       DAWBSB010000275         2645 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_6156_length_2645_cov_3.250579, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000275 DAWBSB010000000
VERSION     DAWBSB010000275.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6156_length_2645_cov_3.250579"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          521 a          834 c          752 g          538 t
ORIGIN      
        1 cgcaccgtgg atacccatgt caaaatgctg cgcagccacc taggcaaata ccgcgacaac
       61 atcgtgacgg tatggggcat cggctataaa tttgaccccg atgtgaggcc cggaaaatga
      121 agcaggacaa gcgccccgcg ggcgcggccg taaaaaagcg gcgttttttc aacaggatca
      181 gccgcaagct gatgctgttt gttgtgctga tgagcatggt gctgctggcg ctggtgtggc
      241 tgctgagcgt aaagctgctg gagcccatgt acaaccggcg cgtgctcgcg gatctgaacc
      301 atgcggcgga cgtctattcc gccatcattg aaaaatacgg caaattcgag agcttcgaca
      361 agagcggcgg cgttcatatt ccctcagaca tgatggagga gatcatggac aaccgggggc
      421 tgctggccgg aaaatgcttt gaggtagccg gaacagactg caaggtcctg ctgcatcttc
      481 accagctgca gtccgaccag tgcctgctgc atcccaatcc gctgagccag cttatggaat
      541 attcagagcc gcagtggaac agcaccgcag ccgtcaacct gcgggccacg accttcgtac
      601 tgggagacct gaactttact ctgccggact cccgcatcct gcccggcctg gaaagcacct
      661 ccggcacccg gcagatggtg gtgtgccgca acatcaacaa ccagtattct ctcatcctct
      721 ccacagacct ggagcgtgtg ggccaggccg ccgacgtcat cagtgcgcaa atgcccatca
      781 ttgccgccgt gctgctcatc gtcagtattg cgggtgctta tgtattcagc cgctggttca
      841 caaaacccat ctcggccatt tcccgcgccg cgcgggagat ggcacgcggc aattacaagg
      901 tgcgcgtcac gccccaggcg gatgacgagc tgggcgcgtt ggccggggat ttcaacacca
      961 tggcccggga ggtgggccgc acctcggagc tgcagcggga cctcatcgcc aatgtcagcc
     1021 acgacctgcg cacgccgctg acgctcatca agggctatgc cgagacggta cgcgacctga
     1081 cgggcgacaa caaggaaaag cgggacgaac agctctcggt catcgtggac gagaccgacc
     1141 gcctctccgg ccttgtgaac agcgtgatgg agctttccaa atattcctcc ggcaccgaaa
     1201 agctcaatcc cgtccagttc gacctggcac agctgtgcga cgaagtggcc taccgctacc
     1261 agaatatctg tgagcagagc ggctacaccc tcacggtaga tgctgccgac ccctgtaccg
     1321 taacggcaga cccggacatg atgagccgtg tggtgcacaa tctgctggcg aacgccatcc
     1381 atcatatcgg gccggacggc tgtgtggcgc tgcgcgtaaa accgcagccg gacgccgccg
     1441 tgcgggtaga gattgaggac cacggcgcgg gcattgcccg ggaagatctt ccctatatct
     1501 ttgacaaata ctaccgctcc cgcgcggacg ccggcaaggt cggcacaggg ctggggcttt
     1561 ccatcacaaa ggccatcctc atcaaccacg gctttccctt cggtgtggac agcgaacccg
     1621 gcaagggcag cattttctgg ttcgtcgctc attgagccgg atatttttct ctgtaaaaaa
     1681 gggacctccc gcacctgcgg gaggtccctt ttattttcca ttaaaatgcc cattctccgt
     1741 tttcaaaaat cttcacgttc tctcccgttc tggtggtacc cgtaatggtc atatccggcg
     1801 cgcccaccat cacgtcctcg tggatgatgc tgtcgttcac accgtgggcc aacagttcct
     1861 gcttcgtcat ggccgtaccg ccctttacag tgccggggta acccttaccg aaggcgatgt
     1921 ggcaggccgc gttctcgtcg aacagcgtat tgtagaacaa aatgccccgc cggttgatgg
     1981 ggctggacgc cggcaccagc gcgatctcgc cgatgcggcg ggagccttca tcggtgtcca
     2041 gcagctggcc cagcagcgcc tcgccgcttt gggccgaatg ctccaccact tcgccgtcct
     2101 tgaaggtgac ggaaaagccc tcaatgaggt tgccgttgta cacatagggc ttggtgccct
     2161 ttacaacgcc gtttacgcgc agacggtgcg gcgcggtgaa cacttcctcg gtggggatat
     2221 ttgcgatgaa tgtgtagccc tcgggcgtac ggctttgagc gccctcccac acatgcccgt
     2281 ccgcaaggcc gatggtgagg tctgtgccgt tggcgctctg cagccggatg ctgtccagat
     2341 ccagcgcatt catgcggtcg cgccaggcgg tcattttttt cacatgcgcc tgccactcgg
     2401 acaccacatt gccgtttttc acgcggcaca cgtcgaagat agcgtcccaa agcattccct
     2461 gggccccatc ctccgccgcg ccgggaaata cctttcccgc ccacgcggcg cttgggatcg
     2521 ccacgatgct ccactgcacc ttgtcgctca tggtatagct gcgccactcc tcctgcgcct
     2581 tcatagccgc ctggtttgcg cggtcgatct tttccatatc aagccccttg tagatctccg
     2641 ggtcc
//
LOCUS       DAWBSB010000276         2645 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_6155_length_2645_cov_3.369884, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000276 DAWBSB010000000
VERSION     DAWBSB010000276.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6155_length_2645_cov_3.369884"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          480 a          762 c          797 g          606 t
ORIGIN      
        1 ggtgcggccg gtgccgccgc cgctggccac agagaagtat tttttgccgt tctccatcgc
       61 ccatttttta taggtgccgg caaccaggtg ctggaagcgg gtggggttgg tagagttgcc
      121 cgtgatggac acctcaacgc cctccttctt cataatggca acgccctcga gcacgtcgtt
      181 ggcgccgtag cacttcaccg cggcgcgttc gccgtcggaa aaagccttct cgcgcacgac
      241 cttcagctcg ccggtcttga agtcgtaatc cgtctccaca taggtgaagc cgttgatgcg
      301 gctgatgata taggcggcgt ccttgcccag gccgttcagg ataacgcgca gcggctcctt
      361 gcgggccttg ttggcggtgc gggcgatgcc gatggcgccc tcggcggcgg caaagctctc
      421 gtggccggcc aggaaggcga agcacttggt ctcgtcgcgc agaagcatgg cgcccaggtt
      481 gccgtggcca aggcccacgg cgcgctgttc ggccaccgag ccggggatgg tgaaggcctc
      541 caggccctcg ccgatggcgg cggcacagtc ggcggcgctc ttcaggccgc gcttcacggc
      601 gatggccgtg cccagcgtgt acgcccagac ggcgttctca aagcagatgg gctgcacacc
      661 cttgacgatc gcgtcaacgt cgatgccctt ggagaggagc atctcgcggc aggcgtccag
      721 agactccagg ccgtttgctt tcaggcatgc ctcgattttc ggcatgcggc gttcttgact
      781 ttcaaattta gccattgttc ttctcctcct ctcttactct tcgcgcgggt cgatatactt
      841 cggcgcgtcc gcaaagcggc cgtaggtgcc gatgttcttc tcgtaagcgg tcttcgggtc
      901 cacaccgtgg cggatgtcct ccatcatttt gcccagacgg acgaactggt agccgatggc
      961 ctcgccgttc tcgtcgacgg cgacctttgt gatatacccc tcggtcagct ccaggtagcg
     1021 cacgcccttc gccttggtgg agaaaatggt gcccgtcatg ctgcgcaggc ccttgcccag
     1081 gtcctccagg ccggcgccga caggcaggcc gtcgtcggaa aacgcggtct ggctgcggcc
     1141 gtagacgatc tgcaggaaca gctcgcgcat ggccacgttg atggcgtcgc acacaaggtc
     1201 tgtgttcagc gcctccagaa tggtcttgcc gggcaggatc tcgcccgcca tggcggcgga
     1261 atgggtcatg ccggagcagc cgatggtctc caccagcgct tcctcgatga cgcccttttt
     1321 cacgttcagc gtcagcttgc aggtgccttg ctgcggcgcg caccagccaa cgccgtgcgt
     1381 caggccgctg atatcagaga tctgatatgc ctttacccac tggccctcct ccgggatggg
     1441 ggccggccca tggttcgggc ctttcgtgag cggacacata cgttctactt catgagaata
     1501 agtcatagtg atgctccttt gtgatgttct tttgtgtttc tcttttgaca gttatttcac
     1561 atttgcgcta tcctatatta taaagaaagc gctgctgcat tgcaagtgcg tggggccgaa
     1621 aatcaaaact ttttccccca ttgtgcgctt ttgcccggtt gcccctgtct tcgcttccgt
     1681 tttgtataat attacaaaaa gttttctata aatttgttga cattaacgat tcacatgcta
     1741 tactgtggtt gacaggggaa cgtcggcctg tcaaacggaa gttgtggaaa atagaattgt
     1801 gagaggagat atgcgatcat ggcatctgtt gatatgcatg atatcgacct gaaagaattc
     1861 aacgaggttc ttgccgcctg caagggcgat gtgtttatgg taacgcccga aggcgaccgc
     1921 ctgaacctga aaagcaggct gtgccagctc atcggcttta ccaagctgat cgagggcggc
     1981 agcatcgcgg aggcgaagct ggagtgctcc gaccccgagg accagcgcag gctgttccgc
     2041 tttaatatgt tcggcgaaaa gtgaaccgtt tcaccttttg gcgcgcccgc atccgcgggc
     2101 gctttttttg tgtccgaacg cgcggatgcg cacggttttt gcagcttcac cgtttgtttg
     2161 tacgaagttg gacggtttgg tttgacaccg tattctataa agtagtatta taaagtggaa
     2221 ttgtgcggcg gcgcgccgcg cggaaggtga gtgaaaaatt tgtctttttt tgaaaaactg
     2281 ttcggctcct tttccacgaa ggagctcaag aagatacagc ccatcgcgga cagggtgctg
     2341 gcgctggagg cccggtacca ggccatgacc gaccgcgagc tgcaggcgat gacgcccgcg
     2401 ctccgggagc gtctggcagg gggcgaaatg ctggacgaca ttctgcccga cgcctttgcc
     2461 gtatgccgcg aggcaagctg gcgcgtgctg ggcatgaaac actttcccgt acaggtggtg
     2521 ggcggcatcg tgctgcaccg cgcctgcatc gccgagatga aaaccggcga gggcaaaacg
     2581 cttgtcgcca cgctgcccgt atatctgaac gcgctcaccg gcaagggcgt gcacgtcgtc
     2641 accgt
//
LOCUS       DAWBSB010000277         2602 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_6265_length_2602_cov_1.742835, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000277 DAWBSB010000000
VERSION     DAWBSB010000277.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6265_length_2602_cov_1.742835"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1105..1114
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1634..1643
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          632 a          656 c          671 g          623 t
ORIGIN      
        1 atatcgttgc cgccgaaccc gatcagcgcc gcttctccct ccgcatgggc agggatctcc
       61 cgctccatta caacgcggcc cttttcactt gtcatgccga atcgcgcatg attctcggct
      121 gggattccga gcgcgcgtga cgaaagcgcc acgcaggacg cttccttcag cctggaatat
      181 cgccgtgtct cagggtccag cacgacgccg cgcaaaatac tgtctcccca cacttcaatc
      241 ttcgatatca ttcaccgcac ctctttatct ggttttaata attagattat cacgttttgt
      301 atttttgtca atctatttcc gcaaagctat tgttccttcg caagcaccgt gatatactga
      361 atttgcaatt taccggcgta tttttgccaa agattcagcc caatcaaaag gagtgccgca
      421 tcatcatgga aataaacaat agtgtcgccg cctggagcgc cgggctggcc gcacaacgtt
      481 tgccgcgttg ggacgaactg ccggacatag acctgtacct cgatcaggtc ctgacgctgc
      541 tggacaaata tctggggcct tttttgcctg aaaacggtgg tcatgcactg acagccagca
      601 tgatcaataa ttatgtaaag ctccgtattg tgcccgcgcc ggtaaagaaa cgttatggac
      661 gcgtccaact cgcttatctg ctcgtaatcg gcctgctcaa gcaggtgctg cccatacccg
      721 atgtgaaaac gctgctggaa agccagtttg aaggcgcgca ggagcagacc gacggcggcg
      781 cggagaaacg cggcttcgcg aaggctttcg actgcttttg cacccttcaa gagaccgcgt
      841 ttgcggatat ggcacggcag gcggcgcagc ttgagaccgc tgccgggacg cgccccgcgc
      901 tggctatgga aatggccgtg cgggccaacg ccagcaaaac gctggcggaa aagctgattg
      961 cgccggacgc acatttgagc aaaacattca agaatgtatc gacgaattaa acaaaagagg
     1021 tgagtacgat ggataacgat ttgtttgact atatcgttag tcttttggtt aggaatgcaa
     1081 acgattcaat cgaggagtgt agagnnnnnn nnnnctatca aagagatttg ggggttcaat
     1141 ggaaggatac acatactatt tcacttatca attgaagcgc acaaagcgaa gattgatgat
     1201 cttaaaaagt tctgtccgaa tcgggatagt ttacattcaa aaaagagagc ttctgatata
     1261 cgtcattggc ggaaagaaat taaaaacgat gtaaaatttt tcggcattgt aaaacagcct
     1321 ccgttttgca gttcgccttt ccggcttcgc aagggtgtgc cgccatgctg tttctattct
     1381 gcaaaatgct gaaaacaagc gcttgggacg ccatataagg tctcaggcgc tttcggtttc
     1441 gcggccttat tggctccgcc cggcctccgt ttgcgaaaac gcaccgcatc tccattcttc
     1501 cctgttttat ttgcgttttt tacattatct ggttataatg attttgcaat gcaattttga
     1561 aagattttgt atgagtaacg ccgtctaaac ggcaggaaag ggcggtggac gccgctgtga
     1621 ttcccgaaca gacnnnnnnn nnncccgata tggatcgtat cgttggcgca tactgcaacg
     1681 ctgtattgcg catgtgtttt ctttatttga aggattcaca ccttgccgag gatgcgtcac
     1741 aagagacctt tttaaaagtg taccgcaact acgcgcaatt cgacggcacc gccggagaaa
     1801 aaacatgggt aatgcgtatc gctatcaatg tgtgtaagga ctatctgcgc agtgcctgga
     1861 accgcagggt cagcgtcgtg gaggcattga atgagatacc ggccgcagaa gattctcccc
     1921 atgaagatga cacgctcctg cgggagatca tgaacctgaa gcctaaatac aaggaagtca
     1981 tcctgctgtt ttattatcag gatatgaaaa tcagcgagat tgcgcgcgtg ctggacgcgc
     2041 cggaatctac ggtttctgtg cggctcaagc gtgcgcgcga gcaattgaaa aagagattgg
     2101 agggatggta tgccgacgga aaataacccg atgaaaagcg cgatcgaccg acgcctttcg
     2161 gcgctttgtg tgcccgatga catggcgcag cgcattcagg cggccgcgca gaaaaaaagg
     2221 ccgcgcgtta tccggttccg ccgtcccctt gccgtggcgg ctgtattttg cctgatatta
     2281 gcctgcagca tgggcgtact ggccaccacc gtcccctctg tccaaaatct gtttgccata
     2341 gtgggcacac agatatcttc catgctgcag cccattgagc gctcctgcac cgacaacggc
     2401 atcgaaacga cggttgttgc cgcgatgaac gacaacgaca tggtggatat ttatgttgca
     2461 gtcaaggatc tggaaggttc acgcatcaat gagcggacca atctggcgga tgttcagctc
     2521 tccggcattg ccatgacgcc gatggtgcag atggtccagt tcgatgaaaa aactgcgaca
     2581 gccaccttcc gcattgaagg cg
//
LOCUS       DAWBSB010000278         2601 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_6267_length_2601_cov_3.185389, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000278 DAWBSB010000000
VERSION     DAWBSB010000278.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6267_length_2601_cov_3.185389"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1977..1986
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          627 a          616 c          746 g          602 t
ORIGIN      
        1 gcgcacaggg ctttgagaac attgaaccat gcaaaaccgc aatagatgcg gttttgttga
       61 ggccgcttcg cggcgttatg gtataatatg tccggttaac gaatgcgggc cgcaggcgaa
      121 gcatgagttt agcggcatag ataccctgtt tcttagtgaa cacgagcggt acgcgaagtg
      181 tgagcttaga aatatggtat actaaagaac aggctgtccc gtgaaccgga acggcaaagc
      241 gcgccccatg cggagcgggc ggcgcgaaga aaaggagtga ccccgtgatg tggtttgaag
      301 aaagcgtgtt ttaccagatc tatccgctgg gcctgtgcgg cgcgccgcgg gaaaacgatg
      361 gcggaacgga gccgcgtatt ttgaagctgc tggactgggt gccccatatc aaaaagctgg
      421 gggcggacgc ggtgtatttc tgcccggtgt ttgaatccga cgctcacggc tacgataccc
      481 gggactatac gaagctggac ggccgccttg gtacgaacgc ggattttgcg cgcgtgtgcg
      541 cggcgctgca cgcggcgggc atccgggtgg tgctggacgg tgtgttcaac catgtggggc
      601 gcggcttttg ggccttccgg gacgtactgg agaagcggga ggccagcccg taccgggatt
      661 ggttccacat tgacttcggc ggcaattcgg gttacaacga cggcctgtgg tacgagggct
      721 gggagggcca ttacgagctt gtaaagctga acctgcgcaa tccggctgtg gcggaatacc
      781 tgttcggctg tattcgcggc tgggtggagg aatttggcgt cgacggcctg cggctggatg
      841 tggcctattg tctggacgag gatttcgtgc gccgtctgcg cgcctttacc ggcggcctga
      901 agccggaatt cgtgctcatc ggcgaaatgc tgcacggcga ttacaagcgc atggtgaacg
      961 gcgccatgct gcacagcgcc accaattatg agtgctacaa ggggctgtat tccagcttca
     1021 attccatgaa cctgtttgag attggccaca gcctggcgcg gcagttcggc cccgaggcgt
     1081 ggacgctgta taagggcctg cctctgctgt cctttgcgga taaccatgat gtaacgcgcg
     1141 tggccacgat tttaacgaac aaagagcatc tgtggccgtt gtacggggta ttgttcggca
     1201 tgcccggcgt gccctgcgtt tattacggca gcgagtgggg catggagggc ggcaaagagg
     1261 acggcgacgc ggcgctgcgc cccgcggtgg acatgccgca gaaaaacggg ctgacggagt
     1321 atattgcccg gctggcggcg gcgcgccggg gcagccgggc actgtgccat ggcgcttacc
     1381 gcacggtgct gctgacgaac cggcagtaca tcttcgagcg cgcggcggag ggcgagcgcg
     1441 tgctggtggc cgtgaacgcg gagggcgaac cgttcgacgc gcactttgac gcgggcgcgg
     1501 ggcgggcggt ggaccttgtc accggaaggc cgcacgattt cggtggcggc agccgtcttg
     1561 cgccgtacag cgcggcttat tggcgcgtgg agtaaacgga cgaggaaaat ggcggagaaa
     1621 aacgcgggcg gccggcggat aaggccggac gcccgcgctg ttttgcgttt tttgaaaaga
     1681 acgaatgcct gataattttg aatttgtcta ttagcctgca tgtgaatgat tatgtatgtg
     1741 cataggatta tatacaaaat gtctatgctc gtgtgtgtgt actatgcgcc catgtttatg
     1801 ttgatggata tggacgtaac cattatataa tttttcattg ccaagcgtat cctttatcat
     1861 aagcaatgta ctgattatca taattccaag agcaatgtaa aattgaaatg tcggtctttc
     1921 gcctaaaata ataaaactaa atccaactcc taaaaatggt gcaacagaat aaaaggnnnn
     1981 nnnnnnaagt aacatagcgg caagcatata ggtgatttca ggcggttttt ccccaacaat
     2041 aaatgcaata aggacactac cgataccaga gaaaattcct ttcaccaaaa cgatttcttc
     2101 tgaactctta tcacttatac tccttgtgca attattctcc acgccccagc atatacaagc
     2161 gcacaataca aacaatgaac ccttgttaaa tacaaatgcc tctgttccct caaaaccaag
     2221 aattgcactg gatagcatta caaggataat tgcggcccat aacctcctgg aaattttttc
     2281 tttaaaaatt acaagagcaa tcacagaagt tgcaacaatt tcaaaattat taatcagcga
     2341 aacattagcg gaatttgtat tagcaatgcc aagcattaag aatataggtg ctgctatgtc
     2401 gagaccaatc attgcaatcg tataaggcaa atctttttta tccagccatt gttcgtcagc
     2461 cagcctgttc tgtgttttcc ttattccaag aagtacacca ataccaacac ctgctcctaa
     2521 atataaaaat cctgcgagca ttctgggcga cacatttatc agtaaaagtt ttgataatgg
     2581 cacattgatt gcatatagtg c
//
LOCUS       DAWBSB010000279         2574 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_6343_length_2574_cov_3.408495, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000279 DAWBSB010000000
VERSION     DAWBSB010000279.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6343_length_2574_cov_3.408495"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          528 a          741 c          845 g          460 t
ORIGIN      
        1 ctcttcacgg cgcggcggtg agctcgcggc cgtatctggg gccgccgtgg ggctgaacag
       61 cggtttcggc acccgcgcaa ggaaagtgag acgaagctat gaataaaaag atcaccgtaa
      121 atctggcggt ggccatcgcc atcattgcca tgacggtcac cttcgttgtg acgatgattc
      181 tgagcatgca gctgttcaac cggaccgtca gcggcgtgcg cgaaaaagag atcatgtaca
      241 acaagctctc ggagatggac aagagcgccc gggagaatta ttacggcgag atcgtggacg
      301 acaccctgta cgattatatc gcggcgggct acgttaccgg catgggcgac aaggacgcgc
      361 gctattatac cgcaaagcag tatctggaat atctcaacca gcagaacggc gtggtcatgg
      421 gcgtgggcgc ggatgtgatc aaggacgcca acggctatgc gcgcattgtt aaggtgtacc
      481 ccaacagccc ggcggaggaa tccggcctgc agaagaattt ctacatcacc aaggtcgggg
      541 agctggacgt taaaacgctt tccctctcgc aggtgaacag ccagctgctg ggcgaatccg
      601 gcacgtcggt gctgctcacg gtggtgaacg ccacgggcga ggacgagcac acggtggaga
      661 tccagcgccg cgagttcgac gtgccctccg tggagggcca ggtgccggag ggtaccacga
      721 ccgcatattt ctggatcaac gctttcaatt ccaagacagt ggacgagctg aaagcatatt
      781 ttgaacgctg gcaggaggag agcgtcgtcg tggacggcgt ggtgttcgac gtgcgcaaca
      841 acacgggcgg aaacctgacg aacgccatgc atgtgatcga ttacctgtgc ccggtaggcc
      901 ctatcgcaag ccagcagaac aaggacggaa ccgtcaccac gctggagacc tccgacgcca
      961 gcgaggtgga gctgcccatg gtggtgctgg tgaacggcaa cacctcctac gcggcggagc
     1021 tgtttgccac atccattcgg gaatttgaaa aaggacgcgt cgtcggtacg cgcacagcgg
     1081 gcaagggtac catccagtgc cgtccggtgg ctctttcaga cggctcggca ttttcgtata
     1141 cggtgggcct gctgctgagc aagaacggag ccaccttcaa cgggacaggc atttcaccgg
     1201 atgtggaggc gtctctctcc gccgaggagg aggcgaattt ctacaacttc accgtggata
     1261 ccgatccgca gatcctgcgc gcgtttgaag tggtggatac gcttacgggc cgcaacgaca
     1321 tccaggccag cctggagccg tcacagagca gctcggcttc ggaggcgggc ggcgcgggtt
     1381 cctctttgca gagcgaaccg gccggaacgg acgcttcgtc ggccgccgag cctgcggcat
     1441 aagcgcggac cggagctgtt tgcaaattga gtctcgcccc tgtatccggg gcggcggaac
     1501 aacgcacacc gcgctgcggg gcgcacagcc cggcgggcgc ggtgcgtttt tgttccgcca
     1561 agaaaaaaga ggaggaagcg cgtgaatctg acggatattt ccaccatcaa aacgctgtgc
     1621 gcggcgcacg gcttttcgct ctccaaggaa ttcgggcaga attttatcgt caacccgggc
     1681 atctgcccca agatcgccga ggcggcgggg attgacgagc gcttcggcgt gctggaggtc
     1741 ggccccggcg tgggggtgct gaccaaggag ctggcgcggc gcgcaaaaaa agtagtggcg
     1801 gtggaggtgg acaagcgtct gccgccgctg ctggcggaaa ctctggcaga atatgaaaac
     1861 gtgacggtcg tggagggcga cgtgctgaag gtggaccttg cggcgctcat ccgggacgag
     1921 ttcccgggcc tgcgtgtggc cgtatgtgca aacctgccct attatattac cagccctatc
     1981 gtcatgaagc tgctggagga gcggctgccc atcgagcata tcacggtaat ggtgcagaaa
     2041 gaggcggcgg accgcctttc cgccgcgccc ggcacccgcg cggcgggcgc tgtcacatat
     2101 gcggtgcatt attacgccgc gccccgcacg ctgtttaccg tacagccggg cagcttttat
     2161 cccccgccca aggtaacgag cgcggtcatt cagctgcgtc tgcacgaagc gccgccggtc
     2221 acgccccgca gcgcggaagc gatgttccgt gtcatccgcg ccgcgttcag ccagcggcgc
     2281 aagacggcgg ccaacgcggc cagcgcgggg ctgcatctgc ccaaggaggc ggtggccgag
     2341 gcgctggctg cggcaggcgt gccgcccacg gcccggcccg agcagctgac gctgcagaat
     2401 tacgccgacg tttccgacgc gctgtttgcg tttcggtgac cggtttgtcc tgttgaaaac
     2461 tgctgttctt ctttgtacac tggtactatg atatgaaata tcaggctccg aatacctgtg
     2521 cagacgaagg aaataacggc ccagagcctg tgcgggccgc ttttgaaacg gaaa
//
LOCUS       DAWBSB010000280         2566 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_6353_length_2566_cov_2.626045, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000280 DAWBSB010000000
VERSION     DAWBSB010000280.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6353_length_2566_cov_2.626045"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2212..2221
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          538 a          826 c          689 g          503 t
ORIGIN      
        1 tttccgtttc aaaagcggcc cgcacaggct ctgggccgtt atttccttcg tctgcgcagg
       61 tattcggaca cggaaacacc ataaaagcgc gaaaatgcac gcttgaacga ataatcgttt
      121 tcgtagccca cggcgcgcgc cgccgccgcc aatgtgattt ttttattttc cagcatctcg
      181 caggccagtt ccatccggcc gctgagcagg aaatcgtaaa aattaatgcc gcacacacgc
      241 ttgaacagct tcgaaaccgc ggagactgaa aggttgaacc ggtctccaag gtccttaaga
      301 gaaagatccg ggtccgtcag atgctcctta acgtattcca cgacctgctg ctccgtatcc
      361 ccagcctgct gagacgctgt tttctccgca cagaacagcg cattcacccg gtacagcgcc
      421 ccccacatgc cgtcatccgt ctgtgcgctg ctgacagcgt caaactcagc ggcatagcgt
      481 gcgggctgcg ggtcgaactc atggatgatg cgcgaatacg tctgcgccag catcagcacc
      541 aaacgccgca tgttctgctg agacacgccc ctgcgctcgt tttcagcgcg tatctcgccc
      601 aggatcgtct ccgccatcgc ctgctggccc gctttcgcac ggttgatgag ctgaagctcc
      661 cactcgacgg gataatagat cccccggtcg ggagatgtaa ggcacgcgcc gtcggacaga
      721 cgtgcagaaa gctgctccgc cgctgcataa taagatttac taatgcccaa aacgccctcc
      781 tccaagctgc cgcaggcgat aaaaggcatc gtttccgtgt gctcctgata cgagagcgcg
      841 acccgctgcg ccagcgtctg cgggcacacc tcatccggcc ctcccggtgc gacaatggcc
      901 gcccgacgcg tcctggaaag ttccgtgatc tcatacaaaa ggtcaaagga tgaaagagcc
      961 tcttccagcg gcatcacacc gtggcttctt tctctttctt ccaccagcag cacaagatgg
     1021 gacatcgaat ctgaaaagcc gatgtcgaac tcccgcattt tttccgcagc ctccgccgca
     1081 tcgaaatacc cctgcagcag ccggcgcaga aaattgctcc gcgcggtggc gcggtaacgc
     1141 tctgccatat gcagcacatt ttcccgttcc gcagccagct ggcacagcga ttcctcaatg
     1201 atcgcatatt catcccccgc atgcaccggc gcgggaaggc ggtgtgccgc gctgtctgcc
     1261 agccgcttga gcggggtgta ggtcagcttt gtaagcagcc acgccagatt caccgtcagc
     1321 agcactgaaa taagcaccag cacggcgata aaccagacgc ttcgtgtccg taccagcggc
     1381 acggcggaaa agccggacgt gatatccacc gggaaacata cggagcggta agtataatca
     1441 gcctctgcgg ccctggccgt gaaggatatc cccgccagct ctctgtccgc gccgcggata
     1501 gaaagggaga gcatatcgtc cgtgcgcagc aatgcgatct ggttgcggat ggcggagcgg
     1561 tccagttcca ccagcagaaa gctccgcatg ggacgattgt actccatcgc ctctgcaaag
     1621 aacagccggg acgccgcgcc gcccgtcatg ccgagctgcg cgccgcacac caggccgctg
     1681 ttttccggca ggctcaaacg ctcgtacacc acagcgctct ccagcggcgt ctgccctttt
     1741 tccagcgaaa atgtgcggaa gaagtctgcc gtatcataaa agccggcgga ggaatacacc
     1801 tcctcctttt cgggaaagac cacagacaat tgccggatca cgccgctggc cgcggtatag
     1861 ccgcgcaggt ctccgcagat ggccagtttc tcgcgcaggc cgaactcttc gtcatacagc
     1921 cccgcggccg caccgcgccg ctttacccac tgcgtctcgg acagctgctg cccaaggccc
     1981 tgaacgcgct tgaactccgc atccagtgtg cgggccgcaa gctcggcggt atgggcctgg
     2041 cgggcggcgt cctcgcgctg ctgtgcgcgg aaggtggaga cggccagcac ccccgccagg
     2101 atgaccaccg gcacaaggca tatgaacaca taggaaaaga agatgcgctg caccgtggaa
     2161 agcctgtgcc gcacggccgg ctttttcttc atcgtgtgcg cccccctccc cnnnnnnnnn
     2221 nacacaggac aaaacggcaa catgtgatcg agaggatgtg gcacattgac aaccatttgc
     2281 aaaaaacgcg gcgcgccgcg gcagagcctc gcgaaccgga tcggccgcag gcggctgcgg
     2341 gagagcggtg agctctatct catcatgctg ccggtgctgg tgatgatctt tatctttttt
     2401 tatatgccca tgttcggcgt tgtcatcgct tttcaggatt acgtccccgg cgaccccttc
     2461 ttcgcgtttg acggctctac aaaatgggtg ggcctgcagc attttaaaac gttcttttct
     2521 tccatctatt ttccgcgcct gttaaaaaac acgctcacgc tcagcc
//
LOCUS       DAWBSB010000281         2544 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_6420_length_2544_cov_2.555645, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000281 DAWBSB010000000
VERSION     DAWBSB010000281.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6420_length_2544_cov_2.555645"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          503 a          799 c          661 g          581 t
ORIGIN      
        1 tatataccgc aggcggcatt tactgttttc cagccaaagc tctttttgcc gccggcgtaa
       61 tatttcacca gcgccaccag cacgccgatg cacacgccga tcaggcacgc cagcgccgcc
      121 agcgctatgg tcatccccaa cccctcgaga tattgcaccc atcggccttt gtacaccgtc
      181 accttataaa aatcgctcag cagcttgttc aagtcgttcc aaaaattttc cacggaggga
      241 tagccccttt ccttatttgc tctgccgcat gcgcccgaaa gctgcacggc ccgcgctgcg
      301 cgggccgtgc gcattttccg cctgccctcc ggacgtcagc cctctgcgca gatcttcaga
      361 tgcttatccg tcagcgactc cactgtgccg tcgtccatcg ccttctgcag cacactgttg
      421 acgacctcca gcaggtcccc gttgcccttg gccacgccca tggccatttc ttcctcggtg
      481 agaagctgct ccacagtttc cagtgcatcc gggttcgcca ttgccagcgt ctgcgcaggc
      541 accttgtcca ggatggccgc gtccgctttt cctcccatca cggccgtagc gcattcattg
      601 gcatttttaa acgccagcgg ttcaatgcca agctcttcct gtgcgtagat gtgagcggtc
      661 gtgctctcct gcacagcgat attctttccc gcaaggtcgt cggctgtctt aatatcgctg
      721 cccttcggca ccagcaggat caatccgttc agtgtataag taatggagaa atcaatttgc
      781 tccgcgcgct cgggcgtagc cgtcataccc gctgcaatga gctgcccctt accgcttttc
      841 agcgattcga tcagcaggtt gaagtccata tccagcactt ccagctccac gcccagctca
      901 tcggccacca actgcgccag gtcgatgtcc acaccggccg gcttgccgtc ctcgcccaga
      961 tattcgaacg gcggaaaacc cgtggccgtc atcatcgtca gcacgccttt ttcctgaatc
     1021 tcctccacaa cggacgtccc gcctgctgag tcctggccgg atgccccgga ggacgcggac
     1081 gcgctgcccg ccgccgggct tgccggagcg gatgcgcccg aaccgccgca ggccgccagg
     1141 cccaacgtca gaaccgctgc cattgcaaat gccaatatct ttttcataac tcccatgctc
     1201 cctctcttgt tcccgttttt tgcgccgccc ctccggcggc ttccatggca ttattatatt
     1261 cgcatattca ttcattgtca acgaattttt atgcgattta tcccttttct gcacattgca
     1321 cagcatttaa ccctccgctt gcgcgtttct aagcatattt atacaaattt gaatttattt
     1381 tatgtatata tttgcattta atctttaaat atgctactat atatacattc tcaaacaaag
     1441 atacggagcg gtaaaaatgc ttgatacaac cttattcaat tcttatctta gcgtaaattt
     1501 gcagacgatc attaataata tgcaaagcat tcaagaggat atcgggccgg atgtggaatg
     1561 catccctgtg ttgaaggaca atgcttactg cctgggtgct gtacccattg ccaaagcgct
     1621 ttgcacctac gcgaacgtgc gcaccatcgc agttgcgcag gtgtgtgagg gcgttgaact
     1681 gcggcaggcc ggattgtccg ctcctgccgt tctggtactg ggcggcgtgc cggagcggtt
     1741 attcccggct gcggtcgagt atgggctgca gctcaccgtg ttccatccgt ccactgtgta
     1801 tgcactggaa aaactggccg ccgcgcaggg aaaacgcgcc gaagtacaga tcaaagttga
     1861 aaccgggctg aaccgcaccg gcgcaaagcc cggcgcagag ctgggcgcgc tggccgccgc
     1921 gctggccgcc tgtccccatg tgcgcacaat gggggtattc tcccattttt ccacgggcga
     1981 gacaaaagaa agtcctacgg cctaccggca gtttcaactt tatcagcagg ccttggcaca
     2041 gcttcaggcc gcaggcatcg atccgccaca gaagcacatg tgcaatagcg gcggcagcga
     2101 ctggttccgc gcctcgttct gcaacgcagt acgcatcggg cgccggctgt atatggataa
     2161 tcaggccgaa ccgctcacac ccgggacgcc gggtgcggtg caggaggcat gcagctggcg
     2221 cgcctcggta acgaacctgc gcgttgtgga ggccggagat actgtgggat atgacgcagc
     2281 ctggacagcg ccgcgccgca cccgcgtcgc caccgtatgc gtgggctacg gcgacggcct
     2341 ctactatcct ctggcggaag caggcggccc gctgctggtg ggggaacaga aagcccggct
     2401 tctggctacc tgcatggacc aatgcttcgt ggatgtaacg gatattccct gccgcgtagg
     2461 agacgaggtc acctttttcg ggcaaagcag cgggggtgca gccatgccca tccaggaact
     2521 cggcaagttc atgcatgtgg aagg
//
LOCUS       DAWBSB010000282         2536 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_6442_length_2536_cov_3.073358, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000282 DAWBSB010000000
VERSION     DAWBSB010000282.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6442_length_2536_cov_3.073358"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          560 a          741 c          636 g          599 t
ORIGIN      
        1 ctgccggacg gatacgattg ccgcgatacc ggaaaagctt catatcactt acgctttctt
       61 ctccggcggg ttgtccttgc gcggctgggc gggctgcccg ctgttcggcg cagaagccgg
      121 ttcgggcttg accaatacga tatcctccgc cttgatatac cagtctacat ctgcattgtc
      181 gccgaacgtc gcgtgcgcca cagcgccgaa tctcgcgccc acaaggtcta ccaccgcatt
      241 atacggatag tcccggaccg ggacgctggc cgggatactc acctggatca tgcggccctg
      301 cgttttgctt ttcagatcat aggtccgttc ttttaccaca tccgaccgcg taccgtcctc
      361 attctgcaca tagacctcac ggcgcatgga agaaaagcgc agctcaccca gcgtagggac
      421 atgtctccct gtaaatccat ttccgaatct tgccataaac aatacccgtc ctttcttcct
      481 tacgccttga tgatatcatc cgcgtggata ataaaatcta tataaccatt cccgttgatc
      541 gcgtaacctt ccgccgtcac ccacgggttg acaagctgca ccggctccat atagtcaaaa
      601 tgtttttcgc ccgcctgcgc cgggatcacg acttcgatat cgtccgcacg ctgtacatcc
      661 gaaaacagct tgtaccgccg ctggacaaca acttcctgcc gcccgacacg ctttttatct
      721 tcacggctgg cctgttccgg cccgccaaat tccagattgc caaggtcttt ttccttgtcc
      781 gggactacaa acagtaaatt cataatgtta cctccaaact gctgttatgg tttattctgt
      841 cattacttct gcgatacccg ccatgatata ctccgcgcag ggcagggcga tcgcattgcc
      901 gagcgcaaag cagcgggcat ggttgctgat gggttcatcc ttgcagccgt atgccgtcca
      961 gccctcgggc aggcccatga gccgttcaca ttccaagggc gtaaggtcac ggataaaacc
     1021 gtcctgctct tttccctcat accagaatgc gaagggctga cctacactgg caagcaaagt
     1081 tgggaagggt tcgccgtttc tgccgaaaca cgtgcggaaa cctttttgat cgccctgctt
     1141 tgccattgac cgcatccgcc ggagctggaa gggacgcacg acgggtacct gccgccctgc
     1201 ttgagcagca ggtattcgat ctccgcgggc ggcgggcagc ctgctttcgc cgcaaggcgc
     1261 aggatacggg agcatactgc ggggcttaaa taatacttct gcggcacgtt ctcctgcaaa
     1321 atccgccacg acgaacaccc gccgcctacg ctgcgaaaga gcgggttctc cccaatactg
     1381 ggcgtccatg agccgccaag cggtatcagc ccgtccccct cgcaccattc cggcgtgcgc
     1441 ccagatacca ctatcaggca ttggaatctc ggtacctgtg aatgcgtgga gcacggctgc
     1501 aaaatccagc cggtcatttg acacaaaagc tcccgcgacg ttctcccaga cagcgaaagc
     1561 tggataccgg ttatctgttg cattcctcat ttcctcaata atacgcatgg cctgatagaa
     1621 caggccggat ttactgcctg ccagaccggt acggttgccg atctgcgaca gattttggca
     1681 cggcgagccg aacgtgacca catccacggg cggtattttg ccgccgtcta tctgcgtaat
     1741 atcgcccaga tgcagcattt cagggaaatg ccgcttggtg acagataccg gcgctctggc
     1801 gatctcgctg gcccacacgg ggacaatgcc acagcgggac gcggcgagag ggaacacgcc
     1861 gatgccgtca aagagactgc cgagtttaag tgcggacacg gccggacctc ttgcgccccc
     1921 gcgcccgcac gccgtccatg ctgttccact ttggggattt cccacggatg cgggagaagc
     1981 agtcacagcg ttcgtccggg tccaaattcg ccccgcagta ggggcaggtg tgataataca
     2041 taagctatcc tcctgattct ggttagttgt tcactctacg ttgattgcgg cggtggccgg
     2101 gcgcattgaa caaatagtaa tctttctcct gatattgtta agataagcac tttatacact
     2161 cctcaatttg tggtaaaata aaaccgccct ctggaatatc caagaggacg gtataaaaag
     2221 gggacaatat gtaattgtaa tgggaactgc gatgccggaa actttcttgt gaatgatgtg
     2281 ttagctgttg ctgccgcccg ttttctgaca ccttgaatga caccaaaggt tctggctggt
     2341 gccgatttga aacggtctga aacaggagtt tctgaaaaaa acttacgctg catttcagga
     2401 acttctgctt ttagaatctc acgttatcag cactattgaa ggcggaaaac gacattcctg
     2461 tcactattga gaacatttct cgtggttgag agaaatctgc tcttgaaaaa ctgcggtttt
     2521 ccaccctctt gtgaat
//
LOCUS       DAWBSB010000283         2535 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_6446_length_2535_cov_3.273387, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000283 DAWBSB010000000
VERSION     DAWBSB010000283.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6446_length_2535_cov_3.273387"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    207..216
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          504 a          648 c          890 g          483 t
ORIGIN      
        1 gctttgggaa aatcgcatga aagagagtct gagcgcacgc cccatccagt ggtttccggg
       61 gcatatgaca aaaacgctgc gcctgatgga aaaggatatc cgcaatgtgg acgctgtgct
      121 ccagcttttg gacgcacgca tcccgcgcag cagcctgaac ccggagattg cgcgcatcac
      181 ggcgggcaag ccccacctgt atgtacnnnn nnnnnngtac agggcgtaaa aaatgcgatt
      241 gagacggaat tggcaaagct tctggagcgc cgtgccgtca agggcatggc gggtgcgaag
      301 atccgcgtga tggtcgtggg catccccaat gtgggaaaat caacgtttat caacagcttc
      361 gcgggcgcgg cgcgcgcgaa ggctgccgac aggcccggcg ttacgcgggg caagcagtgg
      421 gtgaccgtgg gcaattatga ccttctggat atgcccggcg tgctatggaa aaaattcgac
      481 tccatggagg tggcgggcaa cctggccttc atcggttcca tcaaggacga tgtgctggac
      541 atcgaggcgc tggcaacggc gctgctgggt gaaatgcagc ggatgtatcc ggagcgtttg
      601 gcggagcgct accgcctgac agaggaggag ctggggcagg acgcctacga cctgctggag
      661 actgtgggcc gcaagcgggg catgctgatg tccggcggtg tggtgaatac cgagcgcgcg
      721 gccattacgc tggtggatga attccgaggc gcaaagctgg gccgcgtttc gctggaaagg
      781 ccggaggaag catgacggag gaactgtatg cgttcgacgc agaataccgc gcccgtgcgg
      841 gcgtcgtgtg cggcgtggac gaggcgggcc gcggcccgtt gtgcggtccg gtgtgcgtgg
      901 ctgctgttat tctggacccc gcagctccgg tggacggcgt aaacgattcc aaaaagcttt
      961 cggaaaaaaa gagggagctg ctgtatccgc agatcattga aaaggcgctt gcctacaagg
     1021 tcgtgatggt ggggccgcag accatcgacg agctgaatat actgaacgct acgcttctgg
     1081 gcatgcggca ggctgtggag ggccttgcgg tgcgtccggg gctggcgctc atcgatggca
     1141 accggtgccc ggagatggag attcccgcgc aaagcgtcgt caagggagac gcccgttcgg
     1201 caagtatttc ggccgcctct attttggcca aagtctcgcg ggaccggtac atggagcagc
     1261 tcgcgaagga atatcctcaa taccagctgg aaaagcacaa gggatatccg accaagctgc
     1321 attacgcgct gctggacgaa tacggcattc agccgtttta ccgcaggtcc tttttgaaaa
     1381 aacgcggtta tgtgtgagcg gaagagcgcc cgcagggggg ccatgggcga aaggctggcc
     1441 gccgtgtatt accggcgcag aggatgccgg gtgctggaga tgaattttcg cacgcgccgg
     1501 ggtgagatcg acgtcattgc acaaaaagga gacatgctga tctttgccga ggtgaagacg
     1561 cgggacgagg ctgccgccgc gcagcccgca gaggctgtaa cagcgcaaaa gcggcggcgg
     1621 atcgttttgg cggcacaggg atatctgctg cttcacccgg agctggcgga gaattttatg
     1681 cgcttcgacg tggtggaggt cacggtgccc cgcttgggga cgccgcggct gaattgtatt
     1741 gaaaatgcgt ttaccttgta gaaaagaagg ctgtgaagtt ggactttttt gatttgcact
     1801 gtgacacggt gtcccgcctg acggagggcg gcggcatcgc ggatgcggat gcgcacatca
     1861 atctggaaaa ggcgcggaca ctgggccggt gggcgcaggt gttcgccgta ttcgtgcagg
     1921 acaatgcgcc gggcgcggac acggcttatg cttcgtaccg ccttcagacc gcctgttttc
     1981 gggcgcagct cgccgcgaac agagagcgga tcgttcagtg ccgcacagcg caggagacgg
     2041 aagacgcatt tgaagagggc aggcgcgcgg ccattctttc cgtggaaaac ggtgcggcgc
     2101 tgggtgggag ccttgagcgt ttggccgaat tccagcgcga cggcgtgcgc tttctgacgt
     2161 tgacctggat gggcgaaaac gagcttggct gcggcggtga agcgggcggc ccgcttacgg
     2221 agttcggccg tgcagtggtg cgtgcgctgc cgcagtatgg cattgtgccg gatgtttctc
     2281 acctctccga cgagggtgtg gcggaggtgt ttgaatgtta cggtggtccg gtggtggcca
     2341 cgcattccaa cgtgcgccgt gtcacggggc accaccgcaa cctgacggaa aagcagatcc
     2401 gggagatcgt acggcgcggc gggttgatcg gcatcaatct gtgcagtgcg tttttaagca
     2461 gcgatcgcat gcgggcatgc agcgacgacg tataccgtca tctggatgtg ttcctttcgc
     2521 tgggagcgca ggata
//
LOCUS       DAWBSB010000284         2529 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_6467_length_2529_cov_2.670574, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000284 DAWBSB010000000
VERSION     DAWBSB010000284.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6467_length_2529_cov_2.670574"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    809..818
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          543 a          816 c          703 g          457 t
ORIGIN      
        1 gctgttggcc gtccccacat gccttttgat caacctcgtc ctttcaaagc tcttctttct
       61 gccggtgatc gacgtgtggg atatgctggc catttttctc ctgctgatat ccgccgccgc
      121 gctttttatc gccgaccttg cggcaaagaa aaaacatatt gtttcagaga agaaaccgga
      181 gcacagcggc tcgcgcgggc atgcgtgagc tcccgaacag gagggtaaaa ccatgcagca
      241 caaaggaacg gtccggctcg aaacagacag gctcatcttg agaaggttcc ggctggacga
      301 cagcggagcg gcttttcaaa actggaccag tgacaaacgg gtaacggagt ttttgcgttg
      361 gcccgcacac gatacggtca atacgacaaa gaagatcatc caaatgtggg tggacggtta
      421 cgcccgggac gacttctacc aatgggcgat cgttttgaaa aacaacggaa atgggcccgt
      481 cggcacgatc agcgttgttg accggaacga cgcattgaat atattgcata tcggatattg
      541 catcggcagc cgatggtgga accagggcat cacctcggag gcgttttcgg gcatcatccc
      601 atttttattc gagcaggtga aagccaaccg gatcgaatcg cagcacgacc cccgcaaccc
      661 gaattccggc aaggtcatga taaaatgcgg cctgaaatat gagggcacgc tccgtcaggc
      721 ggatatcaac aacaaaggcg tggtagacgc ggccgtgtac agcatattgg ccgacgagta
      781 tttttccaaa caagcgcaat gcaaaaagnn nnnnnnnngg aaggccgccc actgcttatt
      841 tttctttctg cgtttcttcc cgctccggca cagcgcattg ccgcgccgcc tcccgttttt
      901 ttcgccgccc gtaaaacagg gccgaaacgc ccgccgcgca cgccaccacc agcacggcct
      961 ttacccagcc cgaggcctgc tccagcgcgg ccgtaacggc aggccagctt tgcccggcca
     1021 gcgcgcccag atagaccagc aggatattcc acagcaggct gcccacggtg gtgagcagca
     1081 aaaacggcgc cggatgcatg cccgccatgc ccgccgggat ggagatgagg ctgcgcagga
     1141 tggggatgca gcggcagtaa aacacactgg gccagccgtg ggccgcaaac cagtccgacg
     1201 ccttgtgcac gtcggcgctc tccattttca gcacgcgccc aacccggccc gaaagcagcg
     1261 cctccagccg ctgcggggcc agaagccgcc ccacgccgta cagcacgatg gcccccgcca
     1321 cgctgcccag cgtggaaaac agaatgacgc cggggggcgt catctccgta caggtcgtta
     1381 aaaagccgcc gaaggtgagg atcacctcgc tggggatcgg cggaaaaatg ttctccacgg
     1441 caatcagcag cagcacgccc caatagccga accggctgac aaggccggtg atgacgctct
     1501 ccatggcatt cactccctct gcttattcct atgcgggaaa agcggaagga atgctttgca
     1561 cggcgcaaaa agcggcgcgc cccgcggagc aaaaaagccc gccggcggga tgcgcgccgt
     1621 ttcgccggct ctgccgcccg gcgggctttc ccatactcag cccggcgtca gcgcctcccg
     1681 ggcctgggcg gcgctcagac agccccactc caccatctgc tccaccacct ttttctggcg
     1741 ctgccgtgcc agcccggggt tctgcgccgg gtcgtaggcg gagggtgcgt tgggcacgcc
     1801 cgccagcaga gtgcattcgt agggcgtcat ctgcgcgggg gctttgccga aatacccctc
     1861 gcttgcctgg cgcacattat agtatccgct gccgaaatag atggaattca catacagctc
     1921 caggatctcg tccttggtat actgcttctc gatggcccat gccatgaacg cctcggccgc
     1981 cttgcgcgtg agcaccttct cctgtgtgaa atacaggttt ttggcaagct gctgggtgat
     2041 ggtgctgccg ccctccacaa aagcgcccgc gcacacatcg ttccacagcg cacggcacag
     2101 cgcaataaaa tccacgccgc catgggaata aaaacggtgg tcctccaccg ccaccacagc
     2161 gtcccggtac agccggggca gctcgtccag aggcgtaaaa tccggctggg cgcgcacctg
     2221 ctccgccatt tccgccagcg gcttttgctc cagagcctcc cggtacatgc catagccctt
     2281 cagccccacg tacagccccg cactgaggca aagcgcaagc agcaggacaa agagccccgc
     2341 cagcgcccgc ttgatttttc cgcgcatcac cgcgcacctg cctttcccct tccggccgca
     2401 tcctccggct ttgtaccttt attataaaaa ggaaaggccg gcgctgccat cgaactgcct
     2461 ttcattccgc gcgcgcaacc ttacgtttgt gcaagatggc ggcggacaaa aacatgcgga
     2521 gcatacaaa
//
LOCUS       DAWBSB010000285         2496 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_6559_length_2496_cov_3.046702, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000285 DAWBSB010000000
VERSION     DAWBSB010000285.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6559_length_2496_cov_3.046702"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          635 a          659 c          705 g          497 t
ORIGIN      
        1 cttttattct gcctcgtcca tgaaggcgga gaatgaagtt ataaaaaaag agatcgcctt
       61 gagagaagag aggcacgcag cgcaactcaa ggcgagaaag cagcgatcat caaagagctg
      121 cgtgaaaaag gataccaatt gaaatatctg ttgaaatcta tgcagatggc acggtctacc
      181 tattattttg agttgagcaa ggccgatcaa gttgctgttc gaaaccattg cttggcagat
      241 gaaatcaaag acattttctc acagcataaa ggccgctacg gtgtgcgaag agtatatcag
      301 gaactgataa agcgcggcca caaggtcaat cataagcggg tacaacggct tatgcacact
      361 atggggttgg caggaaagcg cccaaaggaa aagtatcatt cctacaaggg cgaggtcggc
      421 aaggttgctg agaatatcct tgaccgggat tgcagcacga cagctcctat gcaaaaatgg
      481 actaccgatg tatctcagtt cggtttttcg tgggggaagt gttatctctc tcctatactc
      541 gacatgaaca caaacgaaat catttcttat gatttatctc aaagtgccaa tatggaacag
      601 atccagagaa tgttggccgg cgcctttacg aagtttccct ctgtagatgg cctgatcttt
      661 cactctgatc aagggtggca gtaccagcac gtgtacttca ggcaggcact taaagatcac
      721 aaaatcattc aatccatgtc aaggaagggc aactgttacg ataattgcat catggagacc
      781 ttctttggac ggatgaaaaa cgagatgttc tacgggcatg aaaaagagta ttcctctttt
      841 gaaacatttg ccaaggctgt tgacggctac ataaattact acaacaacga aagaatccaa
      901 gagaaaacaa aatggatgcc tcctacaaaa ttcagagagg catccatgtg cacctaattc
      961 aattttttat gtgtccagaa ttctgggtac atatcaaaag gctccggccg cgcttttttg
     1021 cgggtaaggc cgccgctggg aaacggcgcg ccgtgtaaag cccgtgtaaa ccggcccgcg
     1081 cccccggcac gcccggcagg cggtttacaa acgctttaca aacacgatac attcccttgg
     1141 ttttacggcg gaggaaggaa ttgtaaaata agggtgtccc aaagggaaag aaaagatttg
     1201 cgaacaaggc aaaggagtaa aaaacgatga aaaagatttt tgcgattgtt ttggccggca
     1261 ttatgatgct gagcctcgcc gcatgcggcg gcaccggctc atccaccccg gccgctgcct
     1321 ccagcgaacc cgcgggcgcg tccggcacgc ctgcttccgg cgagcctgcc gccagcacgc
     1381 tgtccggcaa ggtgaccacg ggcggttcca cctcggttga aaaagttgtc ggcgcgttca
     1441 ttgaggcgtt catggcggaa aacagcggcg tggacgtcac ctacgacccc acgggctccg
     1501 gcgcgggcat taccggcgcg gccgagggaa cgctggacat cggcctttcc agccgtgcgc
     1561 tgaaggacga tgaggccgca ggcggcctga ccgccaccac gttcgcactt gacggcatcg
     1621 ccatcattgt gaacagcgag aacacggtgg aagacctgac cctggagcag atcaaaggcc
     1681 tggccaccgg cgagatcacc aactggagcg aagtgggcgg cccggacatg ccggttgtac
     1741 ttgtgggccg cgaggccggt tccggcacgc gcgacggctt tgaaagcatt gtggacgtga
     1801 aggacgcctg caaatacgac caggagctca cctccaccgg cgccgtcatc gcgggcgttg
     1861 cagcaaaccc caacgctttc ggctacgcgt ccctgtctgc ggttgacgac accgtgaagg
     1921 ccgttaccgt cgagggtgtg gccgccagcg aggcgaccgt gcaggacggc acttacaaaa
     1981 tccagcgtcc gttcgtattc gttacgaagg acggcgcaga gctgagcgag gccgcgcagg
     2041 cgttcatcga cttcgccacc agcgacgccg ccagcgagct gatcgtgaac gcgggcgccg
     2101 tgcctgttgc gaaataatcc acatccgaac aaagcggggg gagccggcgc ggggcgccgg
     2161 ctctttccgg ctatgattca ttgaagatgc gaaaagggtt ttggatatga aagaaacaac
     2221 actggacaac agcatcgaga gcaaacgcag gcgccggcgc gcgctgaaaa aaacgatcga
     2281 aaaaatcatg aacgcggtgt tctttgtctg cggcgcggcg gccattgccg ccgtagcact
     2341 catcaccgta tatatggtcg tctccggcct gccggccatc cgggagatcg gcctgctcga
     2401 cttcctgttc ggcaccgaat ggaagtctac tgccgccgag ccgaaattcg gtattctgcc
     2461 gttcatcctc accagcatct ggggcacgct gggagc
//
LOCUS       DAWBSB010000286         2470 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_6625_length_2470_cov_2.476605, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000286 DAWBSB010000000
VERSION     DAWBSB010000286.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6625_length_2470_cov_2.476605"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    352..361
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          551 a          729 c          684 g          496 t
ORIGIN      
        1 gcctgaagat gggcggaaaa gaaaagcttg ttcgctgggc cgtccgtcag gagctggaga
       61 atccctttga aatcgtcctt ccgctgcggg cgtttgtgga ttgatccacc gaaaaacgta
      121 agcacaataa agctgccgtg caggtttttc ctgcgcggcg gcttttttat gatttggttt
      181 caaactgttt tccgatgatg caaaagcgga gcagcttcca aaagtgtgca cccggggaga
      241 ggtgcatgct tttgtgaatc gcggcggagt cgttgccggg cttctgacga aacggctttc
      301 cggcagctcg ttgtttgagg tgcgccgcgc tgtgcccgcg gggagcacat tnnnnnnnnn
      361 nttcacctcc agaccggatg ctccatgcag caccaacggg atgcgcaacc gctgccggat
      421 gcgatcgatc agctcaatgt tgagcaccgg cgggacggca taaacgccat gtgccgtgcc
      481 tacgcccacg gcaaggctgg aaacccccgt ttccacctca aaacgctcgg cctcgtccgg
      541 gtcggtgtag ccagtttccg agcactcgag gtcgtcctcc ttgccaccga cacggcccag
      601 ctcaccctcc acgggaacgc cgttcgcggc gcacatccgc accacctcgc gcgtcaatgc
      661 gatattctgc accagaggca gcgtacttcc gtcgatcatc acgctggtat accccgcgcg
      721 cagcgcctgt gcgcataatt caaagctgtt cccatgatcc agatgcagcg ccaccggcac
      781 actggcgcgc tgcgcaagcg cctgaacatt actccaatat agctgtgggt ctgcgtaccg
      841 gagcgtcgag ggcgttgtct gcaggattac cggcgcgcgc gcctcctgag cagccagcac
      901 gatggcctgt gccatctcca tgttttccgc attgaacgcg ccgacggcat agccgcccgc
      961 ctgtgcggcg tggagcattt gagtggtgtc ggcaaaaggc atgggggttc ctccttcgat
     1021 ccttctattt tcagcttgtt ttgcgcgtcc cgcgtctcag gcgtggtcca cgacgttttg
     1081 gatccagcgg ccctttttga tgagcagtat gcccaaaaag gttttcagga tttccgtggc
     1141 ctgacagaac ccataaatcc acagaatacc cagccccgtg aaatggacga gacaactagc
     1201 gggtgcaacg catgccagcc agatgaacac ggtgtcgaaa atcaaattca gattggcgtt
     1261 gccgcctgta tggagcacgc cagaaacgac cgtcaggtaa gaggtgaacg gcaggatcag
     1321 tgcactgatg aggataaagg aagaagccag ttgccgcact tcgtcggtcg tgttgtagat
     1381 tagagggaaa tatcgtcccg ccagcgcata aagcagtgcg accgcaaagc tcgacacaaa
     1441 gccaagaccg atgaatttct ggtaaacgga acggattttg tccggcgctt ccgaccggcc
     1501 catcatatgt ccgatgacga ttcgtgccgt gttgccgacg gcgttcgcca caacaataat
     1561 caggctggaa agcgcgctta caatgttgtt tgcgctgaca acatacagcc cgcggacaga
     1621 gtagcactgc gtctgcgcgg caacgcccat ggaccagagt agctggttga gcgcgaacgg
     1681 cgcgcttttt ttcgcaattc gcagggcgag ccagccgggg atatgcagtg agcgatacgc
     1741 gccctgtatg aaggggagcc tgtgtgcgtt gagatgcgtc cagcccgcca cgccggccag
     1801 ctcgatgaat cgcgcgacca gcgtcgcggc ggcggccccg taacagccca tggcagggat
     1861 ccccacaaag ccgaagatca gcacataatt caggacaagg ctcaccacca tggaaaagac
     1921 acagaaaaac atgggcatct ttgtctgccc aagctcccgc aatgtggagg aatagacgtt
     1981 gatgagcgca tacggcggca cgccgactaa cataatccac agatacccca taccataggc
     2041 gagcgacgct tcggcgtctg ccgcaagacc ctcgccgctc aggtagcttt ggatcagcgg
     2101 cttgccccag atgcccagca gtaggatggt cgccgcactc agcaggaaac ttgtcactag
     2161 cttgaaacgg aaagtatagc ggataccctc ctcgtcgcct tttccgctga actgagcagt
     2221 aaacacgccg atcccgccaa aaatccccga aatgcaggaa tagaacacaa acatcagctg
     2281 gttgacaacc gctacgccgt tcatctggac ggtactgacg cgaccgacca taatgttgtt
     2341 cagcagcccg attaggttat aaatgctgcc ctgcatcatg atcggcagcg cgattcgggc
     2401 cgctttcgcg taaaatgcct tttctccgat gtattcccgc accctgatca taaacactcc
     2461 tgccttctcc
//
LOCUS       DAWBSB010000287         2463 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_6650_length_2463_cov_3.182724, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000287 DAWBSB010000000
VERSION     DAWBSB010000287.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6650_length_2463_cov_3.182724"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          425 a          703 c          853 g          482 t
ORIGIN      
        1 gacctgttcc tgaagctgag cgagcgggcc gtgccggcgc acatccccaa ggtgctgtat
       61 tactggcgtg tgcacgaggg ctccacctcc ggcggcacag gggcaaagcc ttatgtggcc
      121 gcggcggcca aacgcgccgt tgcggggcat ctggcgcgca cgggcgcaaa gggcgcggtg
      181 gcggacggct tgtttccaag cacctacaag gtggagtatg ccgtcgaggg aaatccgctt
      241 gtctccatcc tcatcccgaa caaggaccac gcggacgatc tgcgcaaggc gctgacgtcc
      301 atcttcacca aaacggcgta tcccaactat gaggtccttg tcgtcgagaa caattccgtg
      361 gagcccgcca cctttgacta ttatcgggag ctgcaaaagc agcatcccaa ctgccgcgtc
      421 gtgacgtacg agggcggttt caatttcagc gccatcaaca atttcggccg taaggccgcc
      481 cggggcgaat atctgctgct gctcaacaat gacgtggaag tcatcaacgg cacatggctg
      541 ggcgagatgc tggccctgtg cgcgcagccg ggcgtgggca tcgtgggcgc gaagctgtat
      601 tatccggacg ataccatcca gcacgcgggc gtgattgtgg ggctgggcgg ctatgcgggc
      661 cacagccaca aatacgcaaa gcgcggcgga agcggctata tgttccgcgc ggctacggtg
      721 caggatcttt ccgccgttac ggccgcgtgc ctgctggtga aggccgcggt gtacgatgaa
      781 gcgggcggtt tggacgaggg ctttgcggtg gcgtttaacg acgtggattt ctgcctgcgc
      841 gtgcgggcgc tgggctaccg ggtgctgttc acgccttacg ccgagttgta ccactatgag
      901 agcaagagcc ggggccttga cacgaaaggc ccggcccggg aacgctttga gggcgagcgc
      961 cgccgcatga agcggcgcta cggcgaaagc ctgctgcgcg acccgttcta taaccccaat
     1021 ctgacgctgg accgggagga tttttccgag agcgacgccc tgcccaagga cgaccccgtg
     1081 ccgggcgtcc cggcccgtgg atagcgccaa ggcggcgctg cgccgcgtga tgaaagcgcg
     1141 catcgccgcg ctggacgccg cttatgataa ggcggcgggc gagggcatcg tccggcggct
     1201 gacggcccat cccgcctggg ccgcggcgga ttccgtgttc tgttatgtgg gccgcagggg
     1261 cgagatcgac acgcggcccc ttctgcgcgc cgcgctggag gagggcaaaa cgctggccgt
     1321 accgctgtgc atggggccgg gcatgatgca ggcgcgggcc gttcggagcc tggaggagct
     1381 tgcgccggcg gggccttacg gcattccgga gccgcctgcc ggtgcgccgg aggtgcctcc
     1441 gcagcgattg gcgctgtgcg tgctgccctg ccttgccgcc gcgccggatg gaacgcgcct
     1501 gggttatggc ggcgggtatt acgaccgctt tctgccgttg gcatgcaacg cagtgaagat
     1561 cgccctgtgc cgcgcggtgc tgctggagcc tgtgttgccc cgggaatgcc acgacgtgcg
     1621 tgcggatttt gtactgacgg aggacgcgct gtatgcctgc aagggcggcg gctgaggcgc
     1681 cgcgcggaaa gagaggagca gcatgcgagg aacattgacg ctgcccgacg gctatgcggg
     1741 gctgtgcagc atcgacctgc aaaaggacaa gcggctggcg ctgctgatca acgggctggc
     1801 gctgctcatc gcgctgccgt tggtcttttt gggcaaccgg atcgtgccgc tgcgcacgcg
     1861 gttcagtatg gacgatggct tcggctttta ttttctgcgc ctgggtatgc tgacggccgg
     1921 gtatgcggca tatgttgtcc tgcacgaact ggtgcatggc gtctgtatga aatatttcag
     1981 cggcgcgccc gtgcgctacg gcttcaccgg gctgtacgcg tatgcgggca gcgaagctta
     2041 ttttacaaag aagcgctatt ttatcatcgc gctcgcgccg gtggccgttt ggggcgtggt
     2101 gctgctggcg ctgtgcatgc ttgtgcccgc gccgtggttt tggacggtct atttgatcca
     2161 gatcggcaac atcgcgggcg cggccggaga cttttatgtg gtatggcgct ttgccgcaat
     2221 gccaccggac attcttgtgc gggacgcggg agtgggcatg acggtttaca gccgcgggcg
     2281 taaagtacag gctcaatgac atatgcggcg gaagcatctg cgtgattccg ccgctttttt
     2341 gcggacaggg cttgactttt atctgctaca agtgtagtat aaaattatct tctacaaatg
     2401 tagtaaaaac ggcgttatct gccgcatgct gaagggggcg aaaaaatgaa agaaaagctg
     2461 cgc
//
LOCUS       DAWBSB010000288         2455 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_6671_length_2455_cov_3.110833, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000288 DAWBSB010000000
VERSION     DAWBSB010000288.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6671_length_2455_cov_3.110833"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          512 a          722 c          707 g          514 t
ORIGIN      
        1 tatcccgcac acgccgttcg tccgcggcgg ggaaaaagaa cttaccggtc ggggaggtct
       61 ccacgatgcc gtccgtggcc aggcgcttga gggcctggat gaccggcgtg cggctgatct
      121 catactgttc gcaaagacgt tcgatctgca cctgctcgcc ggaatggaac tttccttcaa
      181 aaatgctgga gaggatctgt tcgtaaacgg cctcgtccag attgcgcttc aggcgaaatg
      241 tgttcatatc atacttcctc cagttttatc atcacgagcg atctgctgtt tatgctgtat
      301 agaagtattg tagccgccat tcaatctccc gtcaatatta aattcatttt tgcgctttcc
      361 acaaatcggt ggttcgtttt tctggcttca ttatacacca tgaacaagtt ttgtcaatcc
      421 cgaaattaaa ttaaaaattc agttttctgt tttgcacaat acacaggcgt attacttgtg
      481 cattttgcat gttattccat ccggttacag aattcgatcc gttttgaccg atttccgcag
      541 cgcaaaaaac cgcacgcgcc ggaacaggcg tgtgcgggaa atgagaacgc tgccgttctg
      601 caagactggt cactccggcc gcaatgccgc agaagcggac gcagcgcata aaaaacgggc
      661 aggccatatt cgttgacctg tccgcttcat tttttgtttc ccccactcag gcaacgccga
      721 agcaggtgaa cataataaca agataagcga tatacagcgc ccctgcgagg atgcccatcc
      781 agcgcttgaa cctgccgcag gcaagcgccg ggatcatcac ggccgcgctg atgatgaggc
      841 aggccgggat atcgagcagc atgccctgcc gctcgatggg ctgcgggcgg ccctgcagca
      901 gggcgcacag cggcatgatg agcgtcagat cgaggatgtt cgcgccgatg atattgccga
      961 cagagagcga ggcctgcttt ttgcggatgg agatgatggt cgtgacgagc tccggcagtg
     1021 aggtgccgat ggcgatcatc gtggcggcga taacggcatc cggcacgccg atgcggcgag
     1081 ccagctccga gccgttgtcg atgagcagct gcgcgccgag cacgatggcc gctgcgccga
     1141 gcacgaattt gccgatgttc cacgcgacgg tccttccgtc cttcttcggc ttttccgcgg
     1201 gctgctcagc cccctgctcg cgccgcccgg ccagcagact ctcggccatg tagaaggcga
     1261 atacggccag aatggccgcg ctctcccaca gggagagact gccgtcgcgc gtgaagacga
     1321 acagcaagca gatggcagac agcagcaggc aagccttccc ggcatattgc ctgcgcgtca
     1381 tggcgcagtg catgcataca agggaaatgc agaggatcag gccggtattg gccgtgacgg
     1441 agccgacggc attgccgacg gcgatgtccg cgtttccctc gaaggcggag aacagtgaca
     1501 caagcagctc cggcatggtc gtagcgaagc tgacgaccgt cgcgccgatg ataaacttcg
     1561 ggatgccggt tgactcggcg atccatgttg ccgcgtcgac gaacaggtcg ccgcccttca
     1621 tgatacagaa caggcctgcg ccgaacaggg cgagcacaaa aaacagattg cttccggtta
     1681 gatccagcgg catggacgat ccctccaatg attgcttctg ccgaggcccg caggcgatcg
     1741 gttcgaagat tctgcaaacg taaaagaaaa ccgttgtcaa gtgacaacgg ttttcgattc
     1801 tggagcgggt gacgaggctc gaactcgcta cctccacctt ggcaaggtgg cgctctacca
     1861 gatgagctac actcgcatca gcagggatta taatatcata tcccggctgc tctgtcaacc
     1921 ttaatttttc aaatttccca aaaaatcttc cacggtctgg tgttcccgcg cctgccaggt
     1981 gagcagaatc tccgccaccc gttgctggcc gctgtccggg tagagcttca cctccacccc
     2041 cagaaggggg ggcagggggc aggtctgggc ctggtaggcc tcctggaccg cctggcgcag
     2101 ggtttcggcg ctgtcctcct gggtaaagta gctgatccga aagaccacct tctccggctg
     2161 atcgggcagc agcgcccgga gctcctgctc cacggcggtg ctaccggtga cggagatcac
     2221 ctggctgagc tcctcggggt ccactgcgta ggccagtttg acctccacct ggtaatagga
     2281 gggcgtctgc ttcacatcgt acttgatata gtccaccgcg taagctccca aggggtcctc
     2341 ctgggtgacc tccaggcagg cctgatccac gtcgctggcg gcggtgccgg tgacgctgcc
     2401 gtactggtag agacgcacgg tccccgtctc ctccccctgc tccaccaggt acagc
//
LOCUS       DAWBSB010000289         2420 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_6794_length_2420_cov_2.459619, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000289 DAWBSB010000000
VERSION     DAWBSB010000289.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6794_length_2420_cov_2.459619"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2155..2164
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          565 a          637 c          813 g          395 t
ORIGIN      
        1 gcatctgggc atcggattcc acacggcgct gccacgaaat gcgcaggcaa agcccgtgga
       61 acggaagttc ctagacgtaa aaggccagtt cagccgcctg tgggcgacgt acacgggcgg
      121 caatgtaacg gaaaagccgg aatgcctgaa aaaggtactg aaaaaaggcc atgtacctac
      181 ggacgcggag ttcatcgagg cggtcgacac gctgatccgc ggattctaca acatgcagcc
      241 gtacagcggc cccgtggcag cggaccggaa atacatccgc gaggacgtat acgccatgcg
      301 gatgggcgag cttcgcaagc cggccacgcc ggaggacctg cgcctgatga tgatgcgcac
      361 gacgcggcca cagaccgtgg gccagcgcgg cgttaaagtc aaggtgggcg gcgtctatct
      421 tgagtatttc acacaggact tcctgtggga atggcagaag aaaaaggtgt atgtgcggta
      481 cgacccggac catctggaaa agtgctatgt atacgacaca gacggcaacc ggtttatctg
      541 cgagctgccg ttggaccagc gcctgacgat ggaatggggc gcaacgggcg aggaagtggc
      601 ggaggccgcg cggtatgtgc gccagtacac caaacgcacc aaagaagcca cggaggctgc
      661 gcgcgtgccg ggcctggacc agcaggcatt gatggaaaac cgactggcgg tggcgcggca
      721 gcgcatggac gggtacacac cgcccaaatc cagagcgccg atccggctgg tgcgggcaga
      781 ggatatgaac acggagccgc tgcaggcagc agccagcggc gcgggcgcgg acgtttttgt
      841 cctggcacgc gcggcggagc ggcgaaacaa agaaacggag gaatgacaca atggcaaacg
      901 aacagcttcg ggaacggttg gcgcgctaca tcgaccagta caaggacgat aaggaatccg
      961 gcgtcaatat gggcaccgtg ggccttgcaa tgggctacaa gagcggcgcg gcggtcgtat
     1021 ccgcctatct gtcggggaca tacaaggggc gcgtggatac tttggaggcg cgtctgaagg
     1081 aatacttccg caacgtagag gcacggacag agcaggacag caagctggcg gacattccga
     1141 cgccgaagga atatgtgaag ctctccatct cggagcgcgt attcaccagc atccggcttg
     1201 cccatatgcg cggcagcatc gtggcggtgg ttggtgattc cggtatcggg aagaccaagg
     1261 ccgcgctgga atacaacagc gtgtacccca gctccagcta ttaccttgag gcgacgcctg
     1321 taaatgggaa tctgcggagc ttcctgcggg ccctgtccgc ggaaatgggg ctcccggacg
     1381 gcgggagcaa tctggacctc gaaaaccgca tcaaggagca tctgctgggg atgaacaaag
     1441 tgctgatcat tgacgaagcg cagaacctga agtttacaac catggaaatg ctgaaaagct
     1501 ggagcgacgg cgaccagcgc aaaggtctga agggcgcggg gctggcgctg atcggcaacc
     1561 cggatattga aagccagatg cagagcccga aatacgaccg ccaccgcaac cgcagagtac
     1621 acgttgaaaa atgctggcgg aacagcatca caaaagacga cgtgcggctt ctgttccccg
     1681 cgctggcgaa agaggggcac gaaaaggagt ttgacctgat gtacggcatg tgccggagct
     1741 ggagcgctgt gcgcggcgcg gtgtatgcat atatcaatgc agcggacagc tgccgtgaga
     1801 acggcgtaga gatggattac aacgccctgt ttgcacaggc gcagaccatc aacccgacga
     1861 ttggcgcggg acggatttaa aaacgggtta cagggggatt aaacggatgt ttaacaggat
     1921 gaaaacagtt gtcacattcg cggccggagc cgtggcgggg ctgatgctgg cggcgtgcct
     1981 gacggtgcag ggctcccgcc ccggcgcatt cggcggcgag gcccttattc tgccgctgat
     2041 gggcctgctg ctatatgtgg gctacgaggc cgggcggctg gccacactgg cgcaggcgga
     2101 gcagcggcgg cgcagacgac gggccgggcc gccgtaccgg gtagagcctg gagannnnnn
     2161 nnnnatgctg tcaccgcacg cagaaggtcc agccgctggt ctgcatgcat ctgcaaggac
     2221 ggcttcggcg ggaatggatg ctggatacag aggaaaacga gctgcgggtg tggcctcgct
     2281 gctccgttgc aatagaacac ggaaaggaga atttctgaat ggttgatctc attgcaaccc
     2341 ttatcgctct tgttgtgtac tgtggcttgt catggctcgt gacggcagga attatcaaac
     2401 tgatcacgct gtgctttggc
//
LOCUS       DAWBSB010000290         2400 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_6860_length_2400_cov_2.166311, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000290 DAWBSB010000000
VERSION     DAWBSB010000290.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6860_length_2400_cov_2.166311"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1813..1822
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          476 a          651 c          677 g          586 t
ORIGIN      
        1 gataaagggg gccgaaaaaa tatagcctgc tgttttttct ttataaagcc agccgttttt
       61 tgcgtacccc agcttggaat ttcgctcttt tttcatgttg accctccgcg tcgtttgata
      121 aaaatgccag ccggactttt ggaaaaggcc ggctggcggt atagattcct tactgggcgg
      181 ctgcctcgct cataatgcgg ttgccttctt caaacaattc ctgtgcagcc gcttcagccg
      241 tgatttcgcc gtagtacact ttttcttcaa ggtccgtgat caggctggtg acctcgctgc
      301 tgccggcgcc agcgggcggg ttggccggag agcagttcgg cgtgatgacg tcgttgatgt
      361 aggtgaaaat ctttttctgg atatcagaaa ggcccggctc aatggccttg gcagcctcgc
      421 tggaagcggg cacgcccggc tcggccatga tgatctcgtt cgcttcggga gagttgatcc
      481 accagttcag cagggcgacg gcctggtcga tgttgccgcc ctggttggga atgcaccagc
      541 tcatggcctc gcgcaggtag ttggattcct tcggcttttc caaaggccag gtggtcaagg
      601 caagctcaat tccctccgga gcggcctgct gcatggcatc ggcctggttg gagtaaaaga
      661 agctgcacca ggagctcgtt tccggagtgg tgccgtaaat gatcggctgc tcttcggtgg
      721 cgtccaggcc gaccgtcagg ctgtaatcca tcagccagcc ctcttcacgg ccgcgctcaa
      781 gcagggcgaa atagggctgc agctcttcgg cgctttccac gccgagcttg tctgtctcaa
      841 acaaaatctt gccttcgccg cgcaccaatg cttcgatcat tgtggctggg ttgctgatga
      901 agctgcggta acctgttttt tcgtagatct cggcgcactt ttcagcgaac tggtccaggt
      961 tcatattgtc ctcaatggtg atgccggcct catccaacag ggttttgttg taaatgagcg
     1021 agggagcatt catacccgca cagataccgt acagctttcc gtcgatcgtt gtagcggcca
     1081 gaatttcctc cgacacattg gaaagatcca gcgcgccgct ttccacatac ggcgtcaggt
     1141 cgacgagcag gtcgccgtct acgtactgct ggtaataagc gccctgcagc atggccatgt
     1201 ccggcatgtc attgctggcg gcggctgcgg acagcgccgt caggtggtcg ctgaagccgt
     1261 ttgtctggct ttcgatggtg acgccggggt tctgctccgc gtaaaggtcc agagcgctct
     1321 ggaatttttc attgcgtccc tggctgcccc accaggctac gacaaggtcg cagggttcag
     1381 ccgccgtgcc tgtgctggaa gcgggtgcag cggaatctgc ggatgtgccg gtgctgctgg
     1441 acgtgccgcc gcaagcggcc aggcccaggg ccatcacgca ggcgagcgcc atggcggtga
     1501 tttttttcat cttcataaca ttgctcctcc tttgtttctc tgtaacgttt ttccagaatt
     1561 gggtttaatt taattatata ttttgtatat gtttttccta tttgcaaaac cgaggaaaaa
     1621 cgtaaaaaaa cctacttttt gcagaaaagc ccggcctatt tccaatagga ccaggccttt
     1681 ctgtggggag cgtcttaatt tccgcagcct ctggcgcaat ttttactttt tgcagtccgc
     1741 gcaggcctgc gcgccatatt ctttcatcag cgctacatag gcgcggtaat tttccagcgg
     1801 cgtgcccggg ggnnnnnnnn nngcggatct ctgccggagt gcggaacata cacatcttgt
     1861 caaagctgcc aatcatgagg aaatcggggt acagatcacg gatcttggcc acatccacgc
     1921 cggactggcg ctccagaggc aggataccgt cgatacccgc ctcgatgaac cagggcaccg
     1981 cgctggtaat atcgccgtcg gaatcgatga acacacgggt gccgtgtttc ttgatctccg
     2041 gaatcaggcg acggtaatat ggcagaagaa attcatcaaa aagcgctttg gagagcatgg
     2101 gcccgtggtt gtagctcatg tcctccgcga tggtcatgaa atcaggcttc acatattgcg
     2161 ccatttcgtc caccatccgt acctgccact ctacaagctc gtcacagatg cggtggtaca
     2221 gttccgggta gtcataaaag gaataaaaat gctgttcgat gccgaacagg acgcgcggcc
     2281 accagaaggc gccgtccagc gtataccata caagcgtgtc gccgctttcg tgcggagcgc
     2341 gcagcgcatt cagctgggac gcaaggcggg atactgcatt ttcaggcagc agataggggc
//
LOCUS       DAWBSB010000291         2379 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_6937_length_2379_cov_3.422547, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000291 DAWBSB010000000
VERSION     DAWBSB010000291.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6937_length_2379_cov_3.422547"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          520 a          663 c          576 g          620 t
ORIGIN      
        1 gtgcctctct tctctcaagg cgatctcttt ttttataact tcattctccg cctttatata
       61 ctcgttttct gcccgaagcc ggatcaattc ttctcgttcg gattcattca gtggccttgc
      121 ttcttccttt ttcatttggg gtaccccttt acttggacga ccttttttca tttctaccag
      181 gccattatac ccctttgttt tatagttttg aacccattga tacaatagtc tatcttgaat
      241 accggcctcg attgccactg acacacagga ttttccggac attacttggg atactagttc
      301 cagccgttct tctggtgtcc agttcctgtt gaaatttttg tgtttcagtg cttctggacc
      361 gcggctatcc tctgctctta cccatagtct cactttatcc cgaaaggatt tatcgctaac
      421 accttccggc gtttctggcc atttcccttc tcgatacagt tctacacatt tccttttgta
      481 ctcatatgtg taccgcataa taataccccc tctactgggt gtccagtaaa gggggtacat
      541 atcatttcgc cccggccgcg ccgcctcgct ttattcttct gcaatgcccg cgatgatctt
      601 taccgcctcg cccgccaaaa tcatgcccgc cacgccgggc acccagctca cgctgccggg
      661 cgcggggcgg cccgcggtgc cctttgtttc tccgcccgga cggggcgtca gcgcgggcgc
      721 ggtgctgcac accacgcgca ggcgctccac gccgcgccgc cgcagttccc ggcgcagcac
      781 ccgggccagc gggtcgttct gcgttttata aaggtccgtc acaacaaatt ttgtcgcatc
      841 cagacggttg ccgcacccca tggcgctgac gatgggcacg ccggcctgtg cggcgcgggc
      901 gatcagctcg atcttcgcgc tgacggtgtc gatggcgtcc acgatgcagt cgtattcgga
      961 aaggctgtat ttcggcgcgt cctccacagt gtaaaacacg ggcatgcagg tcactcttgc
     1021 ctgcgggttg atgtcccgga tgcgcgccgc cattacgtcc accttatatt gccccagggt
     1081 agcgtgggtg gctgcgatct gccggttgat gttggagagc gccaccgtgt cgctgtcgaa
     1141 cagcgtaagg cttcccacgc ccgcgcgggc cagcgcctcg gcggcatgcc cgcctacacc
     1201 gcccacgccg aacaccgccg cgtgcgcgcc cgccagacgc tccagggcct gcggccccag
     1261 cagcagctcg ctgcgtctga attcttcccg catcattttc ccttcctttt gcacaaacct
     1321 ttgaaattta gtatagccta aagccgtatg cgccgtcaat cttacgtttt gcaggaccct
     1381 ttttgaggaa agcgttgccg ggatatacct tcggcggttt ctacaaaaac acgttgatat
     1441 agcaggggcc ctgtgctata atcggctcaa caaatctgga tttatgaagg tgataaaatg
     1501 gagaactggc agagtgccga aacgcaaaag gacattgaac agctgatgtc cgcttatggt
     1561 gattttcatg acgcctgtat cgtatctctc aactttcaaa gcggcgcatt tgttgataat
     1621 aaaagcgcaa tgcattttgg cggcccggaa gagcgtgttt tatccgtcgt atttcaacgt
     1681 caatgggaac ccaaaaccat agaactccaa tttatcggtt tgcgccaact acacctgatt
     1741 ggatggcagg acaattacct atgtaatatt ttagaagcat atttggcttt tcatgataat
     1801 ttgctgcctg gcaaacccca tcgtgtaatt gtgtggtctg ataatagttg ctttgatgct
     1861 gctgaaattg gaagtacgat tcaagagcca gcagatacat acattgtgtc aaacgtattg
     1921 agatggcgca ttaaagaata ggcaaaagca gatttatcgg gcagccgtcc atcaaccggc
     1981 aactgcccgt ttgggatatt ttgacgaacg gtttgacgaa gcggctttca gcaacgccga
     2041 tctgaaaaga aagttttgca gcgggcggac gcatataggg acgcatatta aaaaggcgct
     2101 gcttttgcaa caggcccaag cgcttacggc ctgcttccgg gtcatcccac tgcattcgac
     2161 caacagtcga ttgtaatatc ccctgccgtg caatataatt atcccaataa ggaggcgctg
     2221 catcatggac accgaaaaaa tgggacggtt tattgctgag cgcagaaaag acaaacatat
     2281 gacgcaaaaa gatttcgcac gcatgctgaa tattacggac aaagcggtct caaaatggga
     2341 gcgcgggggt tcacttagat aaagatacca caccaatcc
//
LOCUS       DAWBSB010000292         2370 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_6969_length_2370_cov_3.660475, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000292 DAWBSB010000000
VERSION     DAWBSB010000292.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6969_length_2370_cov_3.660475"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          496 a          715 c          726 g          433 t
ORIGIN      
        1 gcgttttgtc accgcttttg caggaccgtc tgctccagca ggcgcgcaac atccgtcaga
       61 ccgggcacga tggcccaggc cagccgctcc atttcgggtg tgggcgggtc catgtccggc
      121 accatcacag tgaagcaccc ggcggcagcg cccgcccgca cgccgttgta ggaatcctcc
      181 agcaccatgc acagcccggg cggtgtgtcc agtgccgccg cggctttcag aaaaatctcc
      241 ggctccggct tggaatgcgt cacctggtcg ccgccgatga cggcggaaaa gtaaccgcgc
      301 acgccggcgg tctccagatt gtgcagcacg cgggcgctgt ctgtggagga agcaacggcc
      361 attttgcagt tccgggcgcg cagcgcgtcc agcagctcga acagccccgg ccgcagcggc
      421 acgctgtgct ccagccttgc ttccacctcg cgccatacct ctgcggacat gtcgtcgtag
      481 gggaacccgg cgccgaagcg ttccaaaaat gcacggcggc cgctttcgta gttgcgcccc
      541 cgcagcagag agacatcctc cggcgtcatc gtcacgccgt atttgcccgc cacggcgcgc
      601 cacacctcgt ctcccagacg ctcggtgtcg aacatcaggc cgtccatgtc gaacaggacg
      661 gcgcggatct gctgttccat tgctttccca acctctcttc attcctgtgc ggcccggccg
      721 ttgccgaacc gcttttcatt atacacgaag cggtgcgaaa agcaaaccgc ccgccgcggg
      781 acggtggagc ggaaatatgc cttagcgcgg aaaaaatggc ggatttacac attttcagcg
      841 gtctgcccct tgtaaaatgg ccgcagggcg gctaaaatag agtaaaacca gcccccgcgg
      901 ttcttttgcg cgccgggtgt ggaactttgc cgctgtgcgg ttttgcgcgc agccatcaat
      961 ggaagaggga aaagtatgct ggacatcaaa gtgacaaaga ccaccgcgcc gaaggcaaag
     1021 ccggacgaga agcatctggg cttcggcaac cattacacgg accatatgtt catcatggac
     1081 tacaccgagg gccgtggctg gcactcgccg cgcatcgagc cgtaccattc cctggagctg
     1141 gacccggccg cgctggtgtt tcattatgcg caggagtgct ttgagggcat gaaagcctac
     1201 cgtgctgcga atggccgcgt gctgctgttc cggccggaaa agaacgccgc ccgcatgcag
     1261 agcacccata ggcgcctgtg cattccggaa atccccgtgg aggatttcgt acaggctgtc
     1321 aaggcgctcg tcagcgtgga caaagactgg gtgcccggag aaccggacac cagcctctat
     1381 ctgcgcccgt tcaccatcgc caccgaggcg catttgggcg tgaagcccag tgcaagctat
     1441 cagttcatca tcatcgcttc gccctccggc gcgtattacg cggagggcat gaatccggtg
     1501 aagatctatg tggaggacga gtatgtccgc gctacgccgg gaggtacggg ctacatcaaa
     1561 tgcggcggca actatgcggc cagcctgatc ggccagatga aggcccatga cctgggctac
     1621 agccaggtgc tgtggctgga cggtgtggag cgcaagtacg tggaggaagt gggcagcatg
     1681 aactgcttct tcaaaatcga cggcgtcatc cgcaccgcgc cctgcgtggg cacggtgctg
     1741 ccgggcatca cgcgcatgag ctgcatcgag ctgctggaaa aatggggcta taaggtggac
     1801 tgtgagagcc gcctgcccat cgcggacgtc atccgcgcag gcaaggaggg caagctggaa
     1861 gaggtgttcg gcacgggcac agcggcggtc atcagccccg tgggggaatt gcgctacgag
     1921 gacgaggtgc tcaccatcaa cggcttccag acaggccctg tcacccagcg cctgtacgac
     1981 acgctcaccg gcatccagtg gggcacgctg cccgacgata tgggctggac gcaggaagtg
     2041 gaataacgcg ggaacggaac aaaacgaacg cggctgcttt acaggggaaa ccgcgtacga
     2101 agagcccctt tccttgcaat acgttttttt tgtgctataa tactcatgcg acaggcgcat
     2161 tcataaaatc ttaatacctt tggaaaaact ttaacaccgc gtttatctgc gcggccggag
     2221 ggaaaaggga cggaaatatg cagatcatta ttgtgggcga cggcaaggta ggtgtcgccc
     2281 ttacggaaca gctctccagc gagggccacg atatcacggt tatcgactca aaccccaagg
     2341 tgctggagca gagcttggaa tcctatgatg
//
LOCUS       DAWBSB010000293         2321 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_7120_length_2321_cov_2.181377, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000293 DAWBSB010000000
VERSION     DAWBSB010000293.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7120_length_2321_cov_2.181377"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2033..2042
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          474 a          751 c          598 g          488 t
ORIGIN      
        1 acggggttat gcgcttcgca gaattccgcg gcgttgccga tatgggcgat cacacggctt
       61 ttgcccgtgt cgatgtcgta gcgaaagaat acctccggta ctgcgccaaa cccatagatg
      121 atcctgcgct tgcgatccag cgcgatggac tggatatacg ccggcgggca cggccgtgcg
      181 agaaacgtaa actccccggt gttgatatca tattttacaa ggcgaccgcc ctttgcagtg
      241 aactgcttgt cgatatcatg gaacagcgca acaccgccat acagcgtatt gccatccaat
      301 tccagcgagc ggtgaaactt ggcgtcaaac ggctcgccca ccttctcgta gccgcaggag
      361 gtgatggttt tggccttacg gtcaaatttc cacatcagct cagctgaaaa actaccgatg
      421 cccagatata cggcgtccga cggctggtga tacgccagcg acgtaatgga aatccatttt
      481 ttcttgtatt gcggatcgcg gacaaaatcg tcatacgtcc accgatccag cacctcggat
      541 ttccagttgt cgaatttttg gtcgtaaatt gcatagtggg aaacattttt cagtttcatg
      601 tttcttcgtc ctttctgcag tttagtttaa gcggttatcg ataacctgct ttccatcccg
      661 tatcacgcct tgcacctgca ggccgctatc cagcagcagc agatccgccc gcttgccgac
      721 ggcgatgctg ccaatctcgg cgtccatacc cagcatacgc gcgggtgtaa gcgccgccat
      781 gcgcaccgca gccgtcagtt caacgccggc acgctgcacc atcgtgcgca ccagccgcga
      841 ggttgtggcg atgctacctg caaagttttt gccgtccggc atcacggcca cgccgccctt
      901 tatgcaaatg ggccggcgtc cctccttatc atcgtacaca acggtgccct ccggcacgtc
      961 cgccgcgccg gcgcggatgg aatcggtaat cagtgcaatg tgctctgggc ctttgatctt
     1021 atagatcagc cgcagaaaat ccggcggcag atgcacgccg tctgcaatga cctccacatc
     1081 catttcatcg atgagaaacg cggcctccac cacaccgccc tcccggtgag gcccgttgcg
     1141 gtgcagcacc gaacagccgg aatacaaatg cgttacgctg tgaaacccgc gtccaaaagc
     1201 ctcgtacacc tgtgcggtgg tggcgtcgga atggccgacg ctggccataa tgccccgctt
     1261 atgcagacgc tcgcccagct ctagcgcacc gggaagttca caggcgatgg accagcggcg
     1321 caagcagggg gcctgctcca gaatacgctc gtattccgcc gcatccggca ggcggatgat
     1381 cgtctcatcc tgcgcgcccc gctccttttg cgaaaaatag ggcccttcca gatggacgcc
     1441 ggccagcctc ggcaacggcg gaagctgcgc ctccctttgc cgcatggcct cgtatatacg
     1501 aaggctctcc agcgtgccct ccagcgttgt gctggacacc gtggccagca ttgtggtggt
     1561 gcctccggcc aggtgggcct gcatggcccg aacaaaggca gcctcttccc catcccggaa
     1621 atcggccccc gcgcccccgt ggacgtgcag gtctacaaag cccggcgtga gatactgtcc
     1681 ttttgcgtcg atcacagtgg aaaacgtttg ctgcggcgaa acgccgcacc catatgctgt
     1741 gatttttccc tcccggctga ttagccaagc gtcctgcagc acctgatagg gcaaaaccaa
     1801 atgcgcatgc tcgattttca ggctcatatt ctcaccctct ctgcagctga tccaggtgaa
     1861 acagcctctt tgcgttcagg cccagaatct gccttttttc ttcatccgtc agctcagcca
     1921 acaaaatgcg gcccactgcg gcctccatag agttccaata ggcgtccgag ccatacagca
     1981 ccttgcaggc gcccgcgccc cgcgccatag cctccaccgc gccgtaccac acnnnnnnnn
     2041 nngccgccac ctcaatggcg ctgcgcaccg cgtcttcctc cccgccggaa tgccccaaaa
     2101 tgaatattgt gcgcgggaag ctttgcgcca gcttatgaaa gcgcagcacc tcttgccccc
     2161 atgtgtgcaa cagcacggga aacccgcgct cgcccgccca tgcataaaca tattggtact
     2221 cggggcactc aaaatccatt ttatgctcaa ctgggtgcag ctttacaccg cgaaaacaag
     2281 ggcaagcggc aaacttttcc atctctgtgc ggtgatcgtc c
//
LOCUS       DAWBSB010000294         2314 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_7153_length_2314_cov_2.156264, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000294 DAWBSB010000000
VERSION     DAWBSB010000294.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7153_length_2314_cov_2.156264"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          451 a          630 c          800 g          433 t
ORIGIN      
        1 aggtcaggga gaaataactg ttgcggatat gggaaaggct ctcgtcaagc cgggtcacag
       61 cgcttgtcat ccgttcgttg gcgctggctt ccgcttcgtg ggtcatggtg ttcagcagac
      121 gcatgtagga gccgagatga aatgcagaga gcacgaagat agccgagaga aagcaggcca
      181 tcaatttaag gttcaaggac aagttgcgcc attttttcaa gggaggggct cctttctgtg
      241 tcaaggtggg gacggcgggc ccgaagtcat aatttattct ttcattctgg ttttgttttg
      301 tcaaggaatt tttaaaaatg agggggaagc agatttatga aaaccagcgt tgcatccgtt
      361 acatttcggc ggaaatccgt ttgtgaggtg gcggagcttg cccgccgcgc cgggctcgac
      421 gccatcgagt ggggcgggga catccatgtg ccgccgggaa acgcccaggc ggcgcgtgcg
      481 gcgctgcact gtacgcggga aaacgggctc accgtatccg cttatggctc gtattaccgg
      541 gcaggtgcag aggaggattt cggtcccgtg ctggaaactg cgctttcact tggatgccgt
      601 gtgatccgcg tttgggccgg gcggcagggc agcgcgctct gcacgcagga agaacggcgc
      661 gccgtgaccg cctgccttgc acgcgctgcg gaacgggcgg cgcaggccgg atgcgtggta
      721 gcgacagagt accatgccga tacgcttacg gatacgctgg cttcagcgct ggctctgctg
      781 ggtgaagcgc cggggctgcg cacgctctgg cagccgcctg ttggccttac ggctgcagaa
      841 aatctgcacg cgctgcacgc gctggaaggc cgagtggaaa atctgcacgt ttatcatcgg
      901 gacagagaag gcgcctgccg ccctctggcg gagggaacag ctgactggca cgcctatttt
      961 gccgcggccc aaaaggacgg cacacagcgg tatgccacgc tggagttcgt gatgggggac
     1021 aatgaggaac aatttttgca ggatgccgcg gcgctgcatg tactgcttaa ggaggaataa
     1081 aaaatgcgta tccgtttgta tgatgttacg cgcataacgc gcacagggct ggtgccccac
     1141 agcgccatcg acgcagagga cggtgcgatt accgctgttt acgatacgct gcctgcggaa
     1201 gagccgggcg tgaggagcgt cgacgcaaag ggcgcatacg ccgcgcccgg tttcatcgac
     1261 atccatctgc acgggggtgg cggggtggaa ttcatggacg ccacgcccga acggattcgg
     1321 gccggctgtg cggctcatgc ccgtcacggc accaccacgc tgctgcccac cacacttgcc
     1381 gcatcgccgg aactgacgct gcgcatgatc cgcgcggtac ggcaggcaca ggccgccaca
     1441 acggaatgca ccatcgcggg cgtccatctg gaaggcccgt tcctttcgcc cgcgcaaagc
     1501 ggggctcaaa gcccggacgc gctgtccctg cccacgtcgg ggctgtggga caggctgctg
     1561 gacgaatggc ccggcggcgt gcgtatcatg ggcgcagcgc ccgagctgcc cggcgcaatg
     1621 gccttgggcg aggcgctgcg ggcccgcggc gtcgtggcga gcattgctca ttcggacgct
     1681 gattacgaga cctgccgccg ggcgctggcg tgcggcttta ccgatatcac ccatatttat
     1741 tccggctgtt ccatggtgca ccgcaaaaac ggctaccgct tcggcggcgt ggtggaggcg
     1801 gggctgctgg aggatgcgtt caccgttcag gtcatcgcag acggtaagca tctgccgccg
     1861 gagctgctgc ggctgatcgt caaatgcaag ggtgcagacc ggatatcgct catcacagat
     1921 gcgctgttcg ctgcaggaag cgatttgccg gacggctcgg tgctacggca ggccaacggc
     1981 atggaaacgg ttttggagga cggcgtgatg aagctgccgg accgtcaggc gtttgcaggc
     2041 agcgtcgcca caatggaccg tcttgtgcgc aatatggtgc atctggcggg ggtgccgtta
     2101 tgggatgcgg tggcgatggc cacggccacg ccggccagag tgatcggcct ggcggacagg
     2161 aaggggagcc ttgcgccggg ctttgacgcg gacatcgtgc tgatggacaa gcagctgaac
     2221 gtgaaagccg ttatggcacg cggacagttt gttgaataga gagaggggaa cgacggaaat
     2281 gtggacggaa gaaagactga acaaaatgct gacg
//
LOCUS       DAWBSB010000295         2283 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_7261_length_2283_cov_3.592460, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000295 DAWBSB010000000
VERSION     DAWBSB010000295.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7261_length_2283_cov_3.592460"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          456 a          655 c          670 g          502 t
ORIGIN      
        1 tggtgagcca tcggggattc gaaccccgga caccctgctt aaaaggcaga tgctctgccg
       61 actgagctaa tggctcataa agaaaaagag aaaagacgga aatactgtat tctccaatga
      121 cgcttgacta ttatatcagc caggtggtat tctgtcaata ccccgccgga aaattgtccc
      181 gccggcagcc gccgtgcgcg caccgacggt gcgcggaaaa gcgcggctcc cggggctttc
      241 ctgcctcctt gaaggcgccg ctcttgtgtg aaatgtttgc cttcctattt acaaaagctt
      301 cggttttgtg tatattgtat aaggatagtt acgctgaaac gctatggtga aaacgggata
      361 gggaacgaaa caaaagataa ggctgcttcg tctatacaga tgccgccttt tcttcttatt
      421 tgcttctttc cgtctcgttt tgccgatcgc gaggttgaca ggcatgacga aacgtttgtg
      481 cagcgccctg ttggcgctga gtacggctgt gatgtgcctt tgcgcctgca ctgcgggcgg
      541 gcaggtctcg gcctcaggcg cgccgccttc cgggagttcc agcgcgccgg cgccttcctc
      601 ggtgtcccag tctgcgcagg agccgcagcc gcagcccgag ccggaaacgc ccgctatcgt
      661 gacagacccg tcggcgaacc ccctcacagg gcttgctaaa gcggatgatt ggcctgaggg
      721 catgcggcct ctggccgtga tgctcgacaa cgttcaggcg gcgctgcccc agcgtggcct
      781 gcagagtgcg gatcttgtat atgaaatggt gaccgagggc ggcatcaccc gtctgatggc
      841 cgtatattcc gattatactt ccatgcccga ggtggggccg gtgcgctccg ctcgggacca
      901 gcatgtgcag cttatgttcc ccttgggagc gatgtacctg catgtgggcg gttccacgta
      961 tgcgaaggcg cttctcagcc aataccatta tgaggccaaa tctttggatg gttattttca
     1021 ggccggcgcg ctggagttgg atgcacagcg caacctgacg gcggccattg agcattgctg
     1081 gttcaccagc ggggagttgt tttctcaggc ggccgcccaa tacaggctgg acaccgagat
     1141 ggacggccca cagcaggccg tgttccagtt cgtcccctac gttcaggagc cccgcgtgct
     1201 ggcgggtggg gaagcgtccc agctctatat ccgtttttcc agctatgcca acagcgcgtt
     1261 ccgctacgat gcagagaaag cgcaatatgt aaaatcacag ttcggcgcgc cgcatttgga
     1321 tgagaacacc ggtgaggccg tcacgttcga taacctgctg gtgctgttca cggacaccga
     1381 aaaatatccc ggcggcattc tcacgaaagt gaattttgcg tggggcggcg tgggctggta
     1441 tttcaacggc ggacgatgcg agccggtacg atggctgaaa ggcgcgccgg aacagccgct
     1501 gcgcatcgtg tccgccgacg gcgccgaaac gcctgtggaa gtcaacccag gccgcaccta
     1561 tattgcggtg gtggggctgg acatgtattc ctatttccgc atcaacgata cggcgcctgt
     1621 ttggcaggcg gcggaataaa acatttttgc ttcaatattt ttggcagcac cgcacgattc
     1681 ccggctaacg ttttgcccgg acaattgtct tgagcggccc ggtactcgga atgacgcagt
     1741 agtgcccgat attgaaaata gatcactaca acaacaggag accatcagat gaaaaaattt
     1801 ctttccatgc ttttgatcct ggctctggcg gcgtccgtgt tcacggcgtg cggcgacaag
     1861 cccgcttcgt ccggcagcac gacggacgct ccagccgctt cctcctcgga gcctgagccg
     1921 caggagccgc cgtatgaccc ggccgtcctc accggcctgc ccaagagtga cgattatccc
     1981 gagggccagc gcgtcacggc tgtcatggtg aacaatatct ccaatacctc ttaccagcag
     2041 gcgcgtccgc agaacggcct gtccgaagcc gacgtgctca tcgagatcaa ggtggagggc
     2101 ggcatcaccc gcttttgcgc cctgtttacc gattaccggg acctgcccga gatctgcccc
     2161 gtgcgcagcg cgcgtgacca gttcttccag cttatcctgc cgctgcagcc gctttatgtg
     2221 catatcggcg agagcgtggt gcagacgcag tatgttaagg attactctta tcaggatatg
     2281 gat
//
LOCUS       DAWBSB010000296         2252 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_7366_length_2252_cov_2.883477, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000296 DAWBSB010000000
VERSION     DAWBSB010000296.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7366_length_2252_cov_2.883477"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          495 a          693 c          613 g          451 t
ORIGIN      
        1 cgcctgcggg atagggccca atgtctcggt gatgaagccg ccgatggtat cacagtcggc
       61 attttcaaaa atgtcctcgc ccaaaaggcc ctccagttca ttcaccggca catcgccgcc
      121 cacctcatac acgccgtccg ccagcgtgac gacggcattg tcgtcctcgt gctcgtcgtc
      181 cagattgccc acgatcgtct ccagcaggtc ctccatggtc accatgcctt ccgtgccgcc
      241 gtattcgtct acgacgatgg ccaggtgcac acgcgctttc tgaaatgcgt gcagcaggtt
      301 ggagcacgtc ataccctcgg gcacatacag cacgcttcgc atgtattttt ccggcgtggc
      361 gtcggcatat cggcccggct caagcagggg gacaaggtct tttacgaaca cagcgcccac
      421 gatatcgtcc aggtcctctt tatataccgg gatgcgcgag aaacggtttt ccgcctgcag
      481 cgccacgatg tcctgcagcg tggccgtcac gggcacgccc accacatcgg tgcggtgcgt
      541 catgatctcg gacaccttgc ggtcgtcgaa ctcgaagatg tttttgatca tctcgtgctc
      601 gcgttcgtcc acggtgcctg tctcctcgcc ggcctccacc atcatcagaa tatcttcctc
      661 cgccaccggc tcctcatcac tggccgggcc gatgcccagc ggacgcagca caaggttggt
      721 ggacgcggcg atcagcttga tgacgggctt gcacaccttg ttcagcgccc atatgacgcc
      781 cacgaccttg agcgccacgc cgtccggatt cttcatagcg atgcgcttgg gcgcaagctc
      841 gccgaaaatc agcatgaaat aagaaagaat aatggtaatg agaaacaccg tcacgccgcg
      901 cagcgtagag gcgggaatgg gaatgaacga cagcgcggag accaatgggt ccgcgaagct
      961 gtccgcggct gcggccgaag ccagaaagcc ggacagcgta acgcccacct gaatggtggc
     1021 caggaagtcc gacggagcct cggtgatgac gagcagacgc gcggcgggct tgctgccgcc
     1081 ctcggccagg cgcttcatct tctgtgtgtt gacggacacc accgcaaact cgctcatggc
     1141 gaaaaacgcg ttggaggcaa tgagtatgac ctgcagaaat aaactgggca ggactgtgtc
     1201 cataaaaatc gatcaatctc cttttttaat aatgggaagc tttgtctttt ttgaagccgc
     1261 gctgaatctc ttcacacctg cagacggaca ggcttgtttt cgcacgcgcc tttgttgcgc
     1321 aggcgctgct ttccgccccg cgcgccgctg cgcaggacag ctaagaaaat aataccagaa
     1381 ccaaagcgga ccgtcaaacg gcgaaatccg tcaaaatctt ggcatcccgg gcggattatg
     1441 gtatactgta caaggttata cttttcttac gtgacattac agggccgcgc gcccattctt
     1501 ggaggtttcg atatggaatc cacagtattt gcaggtaaaa cagtgctcat cacgggcggc
     1561 accggttcct tcggcaatac ggtgctcaaa cactttttat ccacagacat cggcgagatc
     1621 cgcatctttt cccgcgacga aaaaaagcag gacgatatgc gccacgatta tcagctcaaa
     1681 tatccggagc tgagcggcaa gatcaaattt tatatcggcg acgtacgcaa tccggacagc
     1741 ctgcgcggcg tgatgcgcgg cgtggactat attttccatg cggccgcgct caagcaggtt
     1801 ccgagctgcg agttctttcc catggaggcc gtgcgcacca acgtgctggg caccgacaac
     1861 gtattgacag ccgccatcga cgcgggcgta aaaaaggtcg tctgcctctc caccgataaa
     1921 gcggcgtacc ccatcaatgc catgggaatc agcaaggcaa tgatggaaaa agttatcggc
     1981 gcgaaggcgc gcaccgtcaa ggccgaggct accaccatct gctgcacccg ctacggcaac
     2041 gtcatggcca gccgcggcag cgttatcccc ctgtttatcg accagatcaa agcgggcaag
     2101 cccatcacca tcaccgaccc ggagatgacc cgcttcctga tgagcctgga cgaagccgtg
     2161 gaccttgtgg cctttgcgtt cgggcacgcc aaccccggcg acctgtttgt gcagaaaagc
     2221 cccgcctcca ccattgggga ccttgccaaa gc
//
LOCUS       DAWBSB010000297         2246 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_7379_length_2246_cov_3.087175, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000297 DAWBSB010000000
VERSION     DAWBSB010000297.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7379_length_2246_cov_3.087175"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          392 a          677 c          719 g          458 t
ORIGIN      
        1 accgccacct gtcccttttc cagagcgtcc gctgccatgc gcaggggcag caccgccacg
       61 ccaaggccgc gggtggccgc attcaccagc gccagcgtgc tggtggattc ccataccggc
      121 gcgaccgtga aacccgcggc cgcggccgcg ctgtcaaatt gctcgcgggt gccgctgcct
      181 ttttcgcgca gcagaaagcg ctggacgcgg aaggcgtcca gcggcacggt ctggccctgc
      241 gtaaagggac ggcccggcgc gcagacgacg gctagagtat ccgccatata ttcctccgct
      301 gtcagcgcac tgccgtggac aggcccttcc atcagagcga cgtccagcgt gttgtgcagc
      361 agcttttcct caagctgctc cgacggcccc acccagacgc gcacatccac cccggggtgt
      421 gccgcgcgga atgcgtccac ataaccgggc aggaactggg agccgatggt aatgctggct
      481 cctacgcgca gcacgccctg ttcgtcccag ccgcgcaggc ccttttccag gtcgtcgaac
      541 aggccgcaga tgtgcaccgc gtattcccac atgcgccgcc cggcctcggt gagatacagg
      601 cggcggccga tacggtcgaa caggcgcaca ccgtaatatt cctccagctc ggccacggcg
      661 cggcttacgg ccggctgcgc caggtacagc gcccgcgccg cgcgggtggt gttgttgccg
      721 ttttcgcaca cggccagaaa aatgcggatg tgccgcagcg tcatgccaaa atctccttat
      781 aacaaaaata atatgatatt tataatataa tagtattttt attattaaat tgcaaggcgt
      841 atgatgatag ggaaggggga agcgaggtat ggaacaacag ccaaaacaca gcaggaagga
      901 ttatctgacg ctgttcacct ctacgttcac gctcagtgcg ttcacattcg ggggcggatt
      961 cgtcatcatc ccgctgatgc gcaaaaagtt cgtggagcag ctgcactgga tcgacgagga
     1021 agagatgatg gatctgacgg ccattgcaca gtcctcgccg ggagccatcg cagtgaacgc
     1081 atccatctta gtaggctacc gcgtggccgg cgtgcccggg gcgctggtca cggtgctggg
     1141 cactgtgctg ccgccgctca tcattttatc catcatttct tttttttaca ctgcgtttcg
     1201 ggataaccgc atcgtcgcgc tggtcatgcg ggggatgcag gccggcgtgg ccgccgtcat
     1261 ctgcgacgtg gtgctgacga tggggcgctc cgttctgggg gaaaggcgcg tcctgcctgt
     1321 ggtgatgctg ttgggcgcgt ttgctgcggc gcgctttttc ggtgtgaacg tagtggccat
     1381 tatcctggtg tgcggcgcgg tgggcgccgc tgacctgtgg cgcaggcagc gcacctcgaa
     1441 aggaggccgc gcgcaatgat ccatctggaa ctgttctgga gcttttttca aatcgggctg
     1501 ttcagcatcg gcggcggcta tgccgccatg ccgctcatcc agcaccaggt ggtggagctg
     1561 catccctggc ttacgctgga gcagttcagc gatatcatta cgatctcgca gatgacgccc
     1621 ggccccatcg ccatcaattc ggccacgttc gtgggcctgc agctgggagg gctgcccggc
     1681 gcgctggtgg ccacggcggg gtgtgtcctg ccctcctgca ttattgtgct gacgctggct
     1741 tatgtatatt accgctaccg gggcctttcc atggtgcagg gggtgctggc gggcctgcgc
     1801 ccggctgtgg tggcgatgat cgcctccgcg ggcgtgacgc tggtgaacct ttcatttttc
     1861 ggtgcggacg cgctgcccgc agggctcgcg gatgtggacc tcattgccgt gctgctgttt
     1921 gccgccgcgc tgttcgtgct gcggcgctgg aaaaaggacc ccatccttgt gatggcgggc
     1981 gcgggcgcgg cggggcttgt gctgtacacg ctgctgtaag gcattgcgcc cgaatggcaa
     2041 aaaccgcccc ggacggcaaa acgtccgggg cggtttttgc attgtatggc agcggaatgc
     2101 tgcaggaacg gccggggcgg gcctctcaaa aaacgtgcag ggaagcgcct gacgtaccgt
     2161 ctgtgccgag gcttcgcgcg ttctgcaatt ccattgcatg cagcgtattc ccttaaaaga
     2221 gccaaccgaa aaaagcgcaa acgctg
//
LOCUS       DAWBSB010000298         2235 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_7417_length_2235_cov_2.169725, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000298 DAWBSB010000000
VERSION     DAWBSB010000298.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7417_length_2235_cov_2.169725"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          475 a          733 c          554 g          473 t
ORIGIN      
        1 cctcaaacgt catggaaaca agcaccggaa gaggtgtgtt ttcttttgcc gccaaaaccg
       61 ctgcttttat ctcatacaag tctgtcattg tggcgaattt caccagatcc gctccggcac
      121 gccagcccgc caggacaact tccttgtaaa tctcgtacgc ttcttcaaat ttcagcgcgc
      181 ctgcgggctc cagcagttcg cctatggggc caacgtccag catcacacgc gccgcagttc
      241 cggccgcagc acgttttgcc gtcccaatgc ctgcggcgac cacttcttcc accgtatagc
      301 cgcagccggc cagctttttc gcgttggacc caaacgtatt ggcgcaaatc aaatcactgc
      361 cggcctccac atatgcccga ttgatgcgct ctactacatc aggatgtgtg atggaaagca
      421 gatccggcct ttcccccagc ttgaggccgc ttttctggag catcgtcccc atcgcgccgt
      481 ccaacagcca atagcgtttc ccgtcaaata ttttattcac aggtcttccc ctccttacgg
      541 taagcgcaac gttcacgcag cgcacagttt gcgcagcccg cacgacggcc cgtcaccgga
      601 tgaccggcca cgcccaaaac agctgtaacg ctctttcgcg gcgtcagcag atgcgccgga
      661 gtggcgcaca ggccgatttt tcgcggtgca tccagcaaac gcagcagatc gttctgcacg
      721 gcgatgggat aatcgccgta tccaggagaa aaacgccccg tcaaaaactg cccttcctcc
      781 cgttcctcat tgcgcagcgt ctgttcagcg gcatcggccg tctgctccac cgccacagaa
      841 gccagcgcgt ccaacaccac agcataagcc atatcggacg cgcccgcagc acgcagggcc
      901 gcatctactc cggcgcccag tgtacatgcc aggagcacac agctcccgca cccttccaga
      961 tgccgcagga tatcgtttcc ctgcaaataa ggagaaatat catcccgtgc tgcgcgcacc
     1021 gccactgcgc gcggcccggc tgctgcgcgc agcgtcctct ctgcacgccg cagcagagcc
     1081 cgcgtcgcgc tgtccggctc ttctgtgctg atgcccaaat accgcagcgc ctgccgggta
     1141 tcaaaccgct cgggcagtgc aagctccatc ctcatgtcct ctccggcagc agatcgcgga
     1201 ttccctcata tacaagacgc gccacccgtg cgttattcat tgcatataaa tggattccat
     1261 ccgccccgcc tgtgatcagg tcgcggatct gtgtgaccgc gtattcaatg cctgcgtcga
     1321 acagggcctg ttcgtcgtgg tcataccggc tgatcatttt agtaaactgc ggcggcaggc
     1381 tggcggagct gagcgccacg gtgcgctcga tctgcctttt gctcacgatg ggcataatac
     1441 cagcctgcac gggcagagaa attcccgcct tgcgcgccat attcaaaaag cggtaatagc
     1501 tgacattgtc aaagaacaac tgtgtgataa gatgggaaac gcccgcttcc gccttgtagc
     1561 gcaggctctc tacatccgct ttcagcgacg gactttccac gtgcccctca ggatagcacg
     1621 cacctgccac gccgaagccg ccggtctgcg caatggcttt tgcaaggtcg ctcgcatgca
     1681 ggaaatccat ccggcgcggc agatccgggt tgatatcccc gcgcagggca agaacgtttt
     1741 ccacgttgtc ctcgcgcagc cgggtaagtg tttcctccac ctgcacacgc gtggagttta
     1801 cacatgtcag atgcgccaga gcctccacat gaagcccgtt tttgatatac gacgcaatct
     1861 ttgcagtcga ctgtccggcc gtgcctccgc ccgcgccata agtaacgctg atgtaatccg
     1921 gcccaagcgt acacagctct cgcagcaccg gataaagcgt ttcctccgcg gcccctgcct
     1981 ttttgggcgg aaacacctca acggaaaaaa cacagggctt ttgctgaaac atttcatgaa
     2041 taaacatata cgatcccctc tttactgcaa ggcgggcatt ctgccttcaa acatgccgga
     2101 tatgccggtt gaaaaggccc aaaaaccgcc gaagccgcac ggacgcgcgc ggcataaaaa
     2161 tattataagt gagcgctttg ccgatgtaaa gttttttctt acgaatatgc ccacgctttt
     2221 tatatggccg ttgac
//
LOCUS       DAWBSB010000299         2209 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_7507_length_2209_cov_3.158774, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000299 DAWBSB010000000
VERSION     DAWBSB010000299.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7507_length_2209_cov_3.158774"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          423 a          664 c          704 g          418 t
ORIGIN      
        1 gagcgcggcg ctttcgccca aagggcgaaa tgcgaccgga tgggagcaag ccgccccgcg
       61 cgaggatgtg cctttgagcg cggcgttttc gctcaaaggg cgaagtgcgt gcgggctgcg
      121 ggcctggcca gcccggttgg cacagcaaat gcctgccggg gaaaaaatct ggcccggtct
      181 gtttgaaaag cgaaagccgc ttttgccgca gaccgggcgg ggaaagactg tgtcacgcct
      241 gcccgggagc aggcaattcc tccggcggca cgctgtgcac gacgggggag cagacgcggt
      301 ggatgagata atgcagatag tcgatatcct tgaacaaatc cagcccctga gactgggatt
      361 tcattgtgcc ctgcagtgag cgcattgcca caagcaggcc cggcgcttcg tcaaatttca
      421 ccgtcagccc gccgtccggc cggaacacga atttcagcag acgggagctg gagctttcca
      481 gaaaacaaag cgtcactttc aaaacgggtt cgtcgtcctc gttggcggta aagcgccccg
      541 tcacgcccac ggcgaagcgg ttgccaccca agtccagctc gtattccagc gcgttttcaa
      601 agcccacggg aatgcagtac tccacgccgc cttcggtcca gagcagcgaa agcgtgttgt
      661 cccccacacg gaaagctgct ttttccgagc ctttggtata ccagtcctcc atgcagccca
      721 gaatggcggg cagcaggccg gcccggtttt cctccagccg gtatgtttgg gcgagagcgg
      781 ggcgcacgct gtccgggata ggagagggag cagacggcgc ggcagggggg aacagtgcgt
      841 tacggacacg cgcgtaccag ggatgcctga cgggaacagg cggttccggc acagcctcgc
      901 cgaagcgcag atgcgccgcc gtaaaggcaa ggcgcgcgtc tgcggagggg ttggccggca
      961 ggggcgcggg agcacgggcc acggccttca gaaattctct cacagcggtg tagctgcggc
     1021 tgcgggtaaa taaattgccc gcgcctgcgt tcacggccag caccaggtcc aggtccggcg
     1081 caacgacgac atactggccg aacatgccgt tgaacatata agtgtggtcg gcgctgtgcg
     1141 gccagagctg atagccgtat tcttcgccgt tctcatgcac ggtgtcgggt ttgacggcgg
     1201 cttccaccca agcggcgctc aaaaggcgct gttcctttcc gtcggcaccc gtccatttgc
     1261 ccttttgcag atagagttgg cccagcttgg cgatgtcctc cagataaacg tacatgcccc
     1321 agccgccttt ttcgatgccc tgggggcagg tctcccacgc tacatcgccg aagcccagcg
     1381 gctcgaacag gcgcgggcgc agataggccg aaagacccag ccctgttttg cgcttgacga
     1441 tggcggaaag catgtagctg ttcatgctgt tgtaatcgaa tgcggagccg ggctcgaaca
     1501 gaacatctgc gtccagaaag cctttgaccc agtcgccttc cagaaccgcg ccggcctcct
     1561 tgaaagcgac gccgcttgtc atggtgacga ggtgccgcac tgtgacggcg cgcatgcggc
     1621 ggctggtgag caggccgcac ttttcaggaa agatctcgca tacatgctca tccagtgaaa
     1681 ggcggccttc atctgccagc atgcctatgg cggtgcccgt cacgcttttg cacatgctgt
     1741 gcgtcacatg ccaataccgg cttgaatagg gcgcgtacca tccctcgctc accacacggc
     1801 cgtggcgcag tacgatgcag gcatgggtgc cgcactcggc gccggacagg ctgcggtaca
     1861 gcgtatccag cgcggcagca ggcacaccct cgtcccgcgg cgcactgcgc ggcaggagtg
     1921 tgggcagcct gcccgtgcgg ggcggctttt gcggccgcac ggggaacggc aggatgttgt
     1981 ccgtatcgtt cagcagatat ttgcgggcgt aattcagcat tttgatgttg ctgcgcagtt
     2041 ccatgaatgg ttctccctca gcccgggcgc gcgcggagcg gcctgccggg cggtctgtct
     2101 gtaaatattt tccgacaact aaagtatagc atctttgcac agtttttcta taaacagttt
     2161 gtaaagaatg tcgtgagcct gtgtctgcgc ccgcttcccg aacggcggg
//
LOCUS       DAWBSB010000300         2191 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_7584_length_2191_cov_2.416199, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000300 DAWBSB010000000
VERSION     DAWBSB010000300.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7584_length_2191_cov_2.416199"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          461 a          726 c          547 g          457 t
ORIGIN      
        1 ttatacggca catccaaata tttatcgccc ggtttaaaat cggacatact ccacggaaaa
       61 accggcgtat ttcagccgca acctcccgct tcaacgcata tctgtcgcag tcatgctcta
      121 ttataatacc atacccttac agggattgca agaatttttt tctctttttt gcaagaaaat
      181 ttcgtttact ttttgtgcac tatatatatt tttatattct caataaacag tccccaagtt
      241 aagccggatc cgcggcagcc gtcagccttc gcggcccgga cggctgccgg taaccagggc
      301 ggaggggacg aaaagccgcc tgttctccgg cagcgccccc gtgcgccagt aatccagcgc
      361 ggcccgcatg cacaggtagc cctgccggta agcgtcctgc tccaccaaag cggcaatggt
      421 tccattttcc aggtaagcag cgtttttctc cgggttttcg taccccagca ccgccacgct
      481 ggacaggcgc cgcatcttct gcagcgccag gcacacctgc gggacatctc cctgcgacac
      541 atacaccgtg ccgaacgcgc tgccgaagcg ttcactgagc gtacgcgcca gctgtgccgc
      601 cgccacggtg gaagccagca gccccatgtc cacgctgccc agccagcggg cctccggcac
      661 caggctgcct aactcccggc ggaagctctc gtcgcgcgtc cgggccatcg gcatatccag
      721 gccgtgcaca tgtacaatgc cgggcccccg ccggaccgcc ccggcccccg cgcgcgccaa
      781 ccgcatgcca tccttataat aatttgcgcc tacatagaac agctccggga acgccccggt
      841 ttcaacaagg ctgaccaccg gcagcttttc cgtcaacccg cgcaggcgct cctggacgga
      901 aaaaaacggc ggcggcacga caatcagcat atcgggccgc agatcctcca tatagtccat
      961 acagtacagc gcgtctcgct cactgctcag gtcggaaaaa aggctcagaa ccatccccac
     1021 atccccggcc tcccggtccg cggcccgcac ccctctgatc acttctcccc agaaatattt
     1081 cggctttgcg ggcatcacca ccccgatgac cgcctccgcg cggcgcaggc gttccttccg
     1141 ggcaggctca tattgcagct tgcgggccgc ctccagcact gcgtttttca cgccctgctc
     1201 catagacccg caattattca tcgccttgga caccgttgcc ttggacacgt tcgccaaccg
     1261 ggatacggcg tcgattcccc tttccttccg ctctctgtcc atcctgccct ccactccggc
     1321 aaccgtttcc acgctgaaac catgccttaa taaagatgat tctaacatat cagccatgga
     1381 tgcgccatct ttttcaaggt gatttttgac aaaaattatt ttttatcttt agcgcacctg
     1441 cacattacgg aaaactgaac cgtgtgggat aataccggta atgtcgggcg gcacggccca
     1501 cgagccccag ctcttgcttt ttccggcgcg cccgctgctt tcgtttccga gaacgggaga
     1561 gccttctaca gagggggttt ccgtctaaaa cgcactactg aagaaaagag gttgacaaga
     1621 tgtcagaaaa agtaaaaatt ggcgtatttg gcgcggcacg cggcaaatgc atgatcgatg
     1681 ttctcgaata ccaccccgag gcggacctgg tcgccgtgtg cgataaatac cgcccgctgc
     1741 tcgacgaagt ggcgcagtcc gccgagaagg ccggcatcga ggtcgcgctc tatgaagact
     1801 tcgaggactt cttcaagcac gacatggacg cggtggttct ggcgaactac gcaacccagc
     1861 acgcccccta cgccatccgc ttactcaaat ccggtcgcca tgtcctcagt gaggtgctgc
     1921 cctgccagac catggccgag gccgtcgcgc tcatcgaggc cgtcgaggcg tccggcaagg
     1981 tctacgccta cgccgagaac tactgctaca tggaccagac cttcgagatg tggcgccgct
     2041 atgaaaacgg cgatatcggt gaggttgtct atgcagaggg cgaatacatt cacgactgct
     2101 ctgccggatg gccttccatc acctatggcg agcgcgacca ctggcgcaac cgattgttct
     2161 gcacctttta ctgtacgcac agccttgggc c
//
LOCUS       DAWBSB010000301         2187 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_7601_length_2187_cov_3.524859, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000301 DAWBSB010000000
VERSION     DAWBSB010000301.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7601_length_2187_cov_3.524859"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          488 a          673 c          628 g          398 t
ORIGIN      
        1 tgtgggatac cgttactaca caacagtagg taaaaaagtg cgtttcccgt ttgggaaacg
       61 cactcatttt ttcaaagcct cagagcgcct gtaaacagca tcggccgctc agcagagcga
      121 ctgtatactc catgccgtaa gccagcgcgt cttcgtccaa gcgataccgg gcattatgat
      181 ggggagcatc gcttcccttt tccgggtttg ccgcgcccag ctcaaggaaa aaacccgggc
      241 acacctggtc gtaatagcaa aaatcgtctc ccggcgcaat cggtgaaagc tcatgcaccg
      301 cctgcgtgcc aaaggtttgc gcaatcatat ttcgcgccca gccggtcagc gccggatcgt
      361 tgcggatgga atcgtacccg cgcacatatt ccacctgcac atccgcccct gccgaggcgg
      421 caatgccttc gctgatctcg cggatacgcc gctcggcagt ggcgcgcacc cgctcatcgt
      481 agctgcgcac cgagccggtc aaatgcatgc tgccgggtat aatattgtag gccgagccgg
      541 aatgggcctg gcataccgag agcaccgccg ggtcgctggg ccgaagcgaa cgggaaagca
      601 cggtctgcag cgcaaggatg atctgtgcgc cggtcacaat agggtctatg cattcctgcg
      661 gcatgctgga atgtccgccg cggccggtga tctcgatctc aaaacggtcc gtggccgagg
      721 tgagaacgcc gctgcacacg ccgaagctgc ccgtgggcag cgtgcttgta agatgcagac
      781 cgaacacctc gtccacaccc ttcagcacgc cggcgcggat gacttccacc gcaccgccgg
      841 gaggggtctc ctccgcatgc tgaaaaataa agcgcagttc cccgcacagc tcctcccgcc
      901 ggcggcacag cagccgcacg gaattcagca ggatagccgc atgcccgtca tggccgcagg
      961 cgtgcatcac gccctcattg caggagcgat aatccagctc gttttcctcg cggatgggca
     1021 gcgcgtcgat gtccgcacga atggcgaggc agcggccggg ccttccggtg cgcagtaccg
     1081 ccatcacact ggtgggtgta ggacggctga gttccagccc ttccagttcg cacagcgcct
     1141 gctccagata atccgctgtc ttgtattccc ggaaggacag ctcggcatgc cggtgcatcc
     1201 agcgtctgtc ctcgatgcac tgcggcagaa gctccaatac ctgttgatgc agatttttca
     1261 tagccaacgc ctcgatttct tgcagtgatc agaaaataaa atacagtacc ataagcacgc
     1321 caagcaccca ggcgggccac tgcgggacgt acttcgcatc gcggtttaca agcgcgcgga
     1381 tgacgctgac caccagataa gtgaacatgc ccagcgcgat gcccgcgaca tagtcgcctt
     1441 tataggcagt caccagaagc atgacggcca caggcaaaaa ttcagagccc gaaaagtcta
     1501 cgtcgcgcag cgtttccagc atggagatgc ccaccacgat cagcgcgctg ccggttgccg
     1561 cgccgggaat catgaggaac aacgggctga acagcatgca cagcaggaag aaaaacgcgg
     1621 tgctcaggtt ggaaaggccc gtgcggctgc cgctctcaac cccggcggcg ctctccgcaa
     1681 agcagctcac ggtggtgatg ccgaacaggc tgcccaggat ggtggcgctg gagtccacca
     1741 ggaacacctt gccgaaggcc gggaaattcc cgtcctcatc cagcagcccc gctttgcccg
     1801 ccatggcgag gcccgtgccc aatgtgccga agaaatcgtt gatcagcagc atgatgatga
     1861 acggaacaaa actcagtttg agggcgccga gaatatccac ctggaacgcc acattgccga
     1921 tggatgccag cgcaccacgg gtgaagatat tttcgggcaa taccgtaacg cccatgggga
     1981 taccgatgat cgttgtaaga acaatagaga tcagcagtgc gcccttgatt ttataggtgc
     2041 ggccgttgac cgtgattttg agatacgtca gcagcagggc gatggccaaa ccgatcagcc
     2101 ccagctgcac gcttttatcg ctgaaattga tgaagctgcc atcctcaccc gcagcggcca
     2161 ggccgctggt attcaggcag atgctgg
//
LOCUS       DAWBSB010000302         2116 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_7903_length_2116_cov_3.063561, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000302 DAWBSB010000000
VERSION     DAWBSB010000302.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7903_length_2116_cov_3.063561"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          482 a          655 c          619 g          360 t
ORIGIN      
        1 cttctggctg atgcgcccga ataggatctc cagcgcgttt tcccagatat agtccggaaa
       61 catttcgatg caggtaccgg tgggccattt gctttccagc gtctggatat cctgataaat
      121 gatgacggca acatccttgg gcacgcgcag gcccagttca cggaaagcct ccagcgcgcc
      181 ttcggccacc tcgtcactgc ccagcagcat ggcttcggcc agcctgccct cgcgtgcggc
      241 ctgccgggca agagtatagc cgctttcacg gctgatgtct cccacatgga acacagtggg
      301 atcatacagg ccgcgttcct ctaaaatacg gcgcaaaccg gacaggcggc ggccgccgat
      361 gcgcacgccg ccgtgctcgt acacgccccc gatataaccg atgccgcggt attgcttttt
      421 gtccagcagg taagctgcca tctgcttcag gccctggtcg aaatccacct ggatctggtc
      481 gaactcgtaa ttgtgctggt cggaattgac gaacacgatg gcgaaagaca gagaacggag
      541 aaaatccatc tccggcgcgg tgaatgcgcc aaaagcgatc acgccgtcca cgggctgagg
      601 ttggccgaac accagtcgga taaaggaaac gtcgtactgg tccgtcatca tctgcaccag
      661 gcacgacaga gaagaaaggc ggatgttggg cctgtccggc cggatgatgc gccaatcagc
      721 cacgccgatg atgagctttg tcttttccgc cgcagccctg cgctgacggg gagaaacata
      781 gcccagcgca tgggcggcct ggaagatgcg catgcgcacc tcaggcgtga cggacatgtt
      841 gtcatccctg ttcagcaccc gtgataccgt tgtggcagag acctgtgcgc tctgtgcaac
      901 gtctttgatg gtggccatgt gcagttccct cctctggttt tacaatttaa gtataccggg
      961 cacgatgcca aagtcaacta tttttactga ttttttacta aaataaaaca aaaaagaaca
     1021 gcgcgctctg aaggaaacgc gccgtccggc agagtcagtt ttttttcagc agcctttgat
     1081 aggtacagtc gatggcaaac gcgcccagcg gcgcggtgat gaggatggac aatacggcca
     1141 ctgtcagcac gatatcaccg caggcaaggc ccatgcccag cgggatggag ccgattgcgg
     1201 cctgtacggt cgccttggga cagtaggcca acatacagaa tgcgcgctct ctgagggaaa
     1261 ggcgggtgcg cagcaggcac acccatacgc ccagcattcg gaagagaagt accagaaaga
     1321 tgagcagcac cgcctttgtg ccggcgcccg cggcatagcg caggtccact gtggcgccca
     1381 ccagaacaaa cagcagcagc tcggcggcta cccaaagctt tgcatatttg gccgagagcc
     1441 gcgcggcgac ctcaccccgc ttccgctgaa gcgccacacc ggctgccacc accgcgatga
     1501 gaccggaaaa gcccatccgg ccgctgaggc tatcctccag cgtgacgagc aggaacgaaa
     1561 tgctcagcag gatcagcacc tttgcgctgt cccgcagacg aaggcggcgg aacacggccg
     1621 ccagcaggga accgcatgcc gcacccgcca gaatgcccag cacaatggag aacgggatcc
     1681 gcgcaagcgt gcccgccgag ataccgccgc cgtccgcaag cccggtgaag gccgtaaaaa
     1741 gaacgataac gaacacatcg tccaccgagg caccggccat gatcatttgg gggatgccgg
     1801 cgcgcgtacc tctcccttcc tccattagac gcagcatttt gggaacgatc acagcgggcg
     1861 acaccgccgc cacgacgctg cccatgatgg ctgcgtccaa cacagagatg cccagcaggc
     1921 gcggcgcaag cagcaccatt ccggcgatct cgaagcaggc aggcacgaag cagagcagca
     1981 cggcgggccg tccggcccgc ttcaggtcgg caagctccag gttcaggccc gcgcgcgtta
     2041 ggatgatgat gagcgcaatg cggcgaagat cggccgagat ggcaaggatg ctgccatcca
     2101 gcacatccag cgcatg
//
LOCUS       DAWBSB010000303         2088 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_8024_length_2088_cov_3.172159, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000303 DAWBSB010000000
VERSION     DAWBSB010000303.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8024_length_2088_cov_3.172159"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    869..878
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          403 a          526 c          682 g          467 t
ORIGIN      
        1 gcttgccgcc tgcgcgctgc tggacgtgct gccggagcgc tgcttcacgc agggggaggt
       61 gccctctgct tagacaaaat tgcctggatg taaaaaaatg cgacaaccgt ccgggattgc
      121 atatcccgtt gttgtacagg gcatgataca ataacggcag atcctgcaaa aaaggaaggg
      181 acgtttatca tgaagggcat caaatcattc cggcgcacgt ttttattgat tgcggcgctg
      241 tatctggcgt gcggcatttc gctgctggtc tggcccgcgc aatccggcct tgtcatatgc
      301 tatgtgttcg gcggcatatc gctgctgtat ggggcggtac agatcatccg ctattgctgc
      361 aacgctgccg tttccatttt ccgctatgaa cttgtcacag gcattctctg ctgtgcgttt
      421 ggcctgtttg ttatcctgcg tgcggacatg gtgctggcgg tactgccgtt tgtgttcggc
      481 tgcgccatcg tgtttgacag cgtcatgaca ctccagaacg ccattgacct gcgccgcctg
      541 ggtttttccc gctggtggct gatcctgctg ctggcgggcg ttaccggcgc gttcggcgtg
      601 ctgctgtttt tcgaaccgtt cgcggcgctg gccatggtgc gcttcatcgg tgcggtgttg
      661 gtgctggacg gctgtgcgga tcttgtcagc gtgctgtgtc tgacctactg ggtaaaaaag
      721 gcgaagcgtg gcctggaggc gatttatgac gaagcccgtt acgaagcagc cgccatcgag
      781 gccgagggcc gcatccagga cgagtccgag ccgtgaggca gagcagaaca aaaaagcggc
      841 cgggccgccc tgcggggcgg cctgttttnn nnnnnnnntg taaaaagagg cggcggcagg
      901 ctgggacccc cttgtgcacc aatgctgtcc ggtgtaaaat caaagaaagc acagagcgcg
      961 aacgcgccgt gtgagaccag gcgatagaga aataacggat ggcgcgggtc tgtacgctct
     1021 cctgccactg cggccccaat tgtatgggcg tcctgctcat ccgcagtgca taacacagcg
     1081 atgtgcttct gcgcgtccgg tatcaagaaa gggaattttt atatgatacg tttggtggcg
     1141 agcgacatcg acggcacgct gctgcagggc ggcgcgcgag cgctttcgga cgagctgttc
     1201 ggccttatcc gggtgttgaa gaagcgcgga attctgttct gcgcggccag cgggcggcag
     1261 taccgcagca tgcgggcgct gtttgcacag gcggaggatg atatcgctta cctttgcgaa
     1321 aacggggcca ttgtgtaccg gggcgggagc gtggtgtcta agctggccat ggagcgcgcc
     1381 gatgcgctgg cgctcattgg ccagattcag gcgcaggagc aatgcgaggt gctcatctcc
     1441 ggcgcggaca ccagctatct gctccccaag catccggatt atgtggacca tatccgctat
     1501 tttgtgggca atcacattac gcgggtgaag cgggcgcagg acatcccgga ggatatcctc
     1561 aaggtgtcgg cttactgccg ggatggcgcg gcgagccatg cacagacgct gggcgcgccg
     1621 tggaaagcgg cgtacagcgt ggctgtggcg ggggaaaaat ggctggattt cacgcgcgca
     1681 gacaagggca cgggccttgc tgccctgtgc ggggagttgg gcgtgcgccc gcaggaggtc
     1741 atagcgttcg gtgacaattt caatgatgtt cccatgttgg accttgtggg cagcccctat
     1801 atcatgcaag gggccgtacc cgcgctgctg gagcggtacc cgatgcagtg tgcccgggtg
     1861 gaggatgtgt tgcgaaaact tgcagagggg tagcactcgc tatttttttg gcttgtttgg
     1921 atattggaat aaaaaaatta cgaaatacat aaaaaatata ctaacgcttt gcggcaatga
     1981 gatgatacaa taaaattgat atccacaaga gaagccgcgg tatggtgctc cgggcggagt
     2041 gttccggggg cggacgggct tcggataaaa gttgttttgc ctgacaga
//
LOCUS       DAWBSB010000304         2049 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_8181_length_2049_cov_2.517553, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000304 DAWBSB010000000
VERSION     DAWBSB010000304.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8181_length_2049_cov_2.517553"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          447 a          539 c          613 g          450 t
ORIGIN      
        1 ggtttcctcc gtgcacggcc acggcacggg cgacctgctg gaagcggtgt gcgcctgcct
       61 gcctgggaat gaagatggag aggatgagga cgagcgcatc ggtgtggcgg tcatcggccg
      121 ccccaacgta ggcaagtcca gcctggtcaa ccatatcctg ggcgaggaac gcatgatcgt
      181 cgccaacgag gcgggcacca cccgggacgc ggtggacagc gatgtggaca accagtacgg
      241 caaatttacg ttcatcgaca ctgccggcct gcgcaaacgc ggcaaagtgg aggacggcgt
      301 ggagcggtac agcgtgctgc gcagtctggc ggccgtggag cgcgccaagg tgtgtgtcat
      361 catgatcgac gccacagagg gctttaccga gcaggattcc aaggtggcgg gcttcgccca
      421 cgagcagggc aaggcgtgca tcattgccgt caacaaatgg gacgcggtgg agaaagacga
      481 taagaccatg gatgtacagc gcaaaaagct gatgaacgat ttcagcttta tgtcttacgc
      541 acccatcatc ttcatttcgg caaaaacggg ccagcgtgtg gatcgtttgt ttgagctgat
      601 caagtttgtg gacagccaga acgccatgcg cgtgaccacg ggcatgctga acgatctgct
      661 ggcccgggct acggcgcgcg tgcagccgcc cagcgacaaa ggcaaacgcc tgaagatcta
      721 ctacatgacc cagatcgcca cgcgcccgcc cacgttcgtc tgtttctgca actccaagca
      781 gctgttccat ttttcttacg cgcgttatat cgagaatcag atccgcgaga ccttcggcct
      841 ggaggggacg cccgtgcgca tcgttatccg cgagcgaggg gaaaaggtgt agctgcccgt
      901 gcagggccgg aagagcgtgc cgcgcggcac gccggaacaa ggcggcgggc gtgtgcccgg
      961 cgctgaatac aggagggatt accgatgctg caaaccgtgc tgtgcggcgc ggcatgtgct
     1021 gccgccgcgt atctgcttgg ctctctgagc ttttccatca tcgtgacaag gcttttgtat
     1081 cacaaggaca tccgcacctt cggctccggc aacgcgggca tgaccaatgt gctgcgcacc
     1141 tttggaaaag gcgcggcggc gctcaccatt gtgggcgaca tcggaaaagg catcctggcg
     1201 gtgctgctgg cgaaatgggc gttcggtgcg tttacggcgg cagaccccat ctacggtgcc
     1261 tatatcgcgg cgttctgtgc ggttctgggg catgtgtacc cggtatattt cggcttcaag
     1321 ggcggcaagg ctgtatctgt ggccacgggc acgatcattg ccatcaagcc ggtgcttatt
     1381 ttgcctttgt tggcggtgtt ttttatcgtg ttcctgtgca gcaaaatggt ttctctggca
     1441 agcatctgct gcgcggcggc gtatcccgcc gttacatttc tgtattttta ttttgtggcc
     1501 gggcgcgacg tcgtgctcac cacttgctgc gcggcggtca tcggcggatt ggttatatgg
     1561 ctgcaccgga gcaatatcca gcgcattaaa aacgggacgg aatacaagtt cgggcagaag
     1621 aaaaaataga aaatgcgggt gtgaaaacag ttttcacacg cgcatctttg tttttgttgg
     1681 gaaaatttat atggaaaatg atgaaaaaat accccgcaca cggatgtgtg cggggtgcag
     1741 ttgttcgcta tcagacagcg cgggtaactt tacccgaacg caggcagcgg gtgcaggcgt
     1801 ggatataacg gggagaaccg tcaaccacag ctttcactct ttttacgttg ggcttccaag
     1861 ctctgtttgc acgtctgtgc gagtgggaca ccttgatacc aaaggtcacg cctttcccac
     1921 agcaatcaca ttttgccatt tactgcacct cctcttttca agtcgctaga atataatatc
     1981 aaataatccg gcaaaaagca agtttttttc aaaaataaat cagagatatt ttacacacta
     2041 tttgataca
//
LOCUS       DAWBSB010000305         2027 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_8285_length_2027_cov_2.334686, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000305 DAWBSB010000000
VERSION     DAWBSB010000305.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8285_length_2027_cov_2.334686"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          381 a          584 c          624 g          438 t
ORIGIN      
        1 cgggtacata tcggtatgga tgaagcctgg tcgttggggt tgggaaatta ccgcttcaaa
       61 aacggctttg tcccgtcaca ccaactgatg gaacagcatc ttgcccgcgt gtgcgccatc
      121 gcacagaaat acagtctgca gccgatgata tggagcgaca tgtacctgcg ggcggcctcg
      181 cctacgggag gttactacga tgttcccggg cagctgcctg cggatgttct gagcgcggcg
      241 cgtcgggatg tccccctcgt ttattgggac tactaccata acgagcagcc ggtgtacgag
      301 catcttctgc acgtccatgc ccagtttggt gcgcccgtgg tgtttgcggg cgggctttgg
      361 acatgggtgg ggcccgcacc cgctgtggat aagatgcgcg cgtcttctct tcccgggctg
      421 cacgcctgcg aaacgaatgg gatacgctcc gtatttgcca ccgcctgggg cgacaacggc
      481 ggcgaggcca atttgctgac agcgctttac ggcatgcagt tgtatgctga gtacgattac
      541 accggagata cgcggccgga cgttgtggac gaacggtttc gcgcctgcac cggagaggac
      601 ccggccgcct ttgcgtacat gggagatttc aacactcctc caggcgtcgc cgccaaaagc
      661 gaagatcctg tgaacgccgc caagtttttg ctgtatgagg accctctggt gccgctgttc
      721 gccgccgata cggcgggcat gtccttttcc gggttttatg cggatcttgc gcgtaaatat
      781 gaaaatttct ccagcgactc gccggaattt gaggcgctgt accggtttta tgagcagctt
      841 gcccttttgc tggagctgaa atgccgctgg cgggagcagg caccccgagc gaaggacggc
      901 accgcgtctg cgcttgcgca gctggccgga gcgtgcgcac ggcagaccct gcgctgcaag
      961 cgcgcctggg aggctttgtg ggactgcacg aacaatccct tcgggtttga agttatcgac
     1021 ctgcgcctgt ccggccttgc ggggcggttc gataccgcgg cgcgccggat ggaacgcttt
     1081 gcagcgggtg acgcctccgc gctggagacg cttttcagcc cgaagctgcg gtatcttaca
     1141 gatagcgccg gacatttttc cggctgctat tcctgggccg aatgcatttc tgcctgccgg
     1201 atctgaaaag gaggagtatg atggaacaac cgatcaccgc cgttatcggc ggactgggcg
     1261 taaggggcta tgaggtgtac gcttcctacg cgcgccgcaa tcccgacaaa atacgcattg
     1321 tagccgttgc agacccggac ccggccaagc gcacagccgc gcgggaggct ttcggccttg
     1381 ccgaagaaag ctgttatgaa agcatggaag ctctgttgga agcgccgcgc ctgggcgatg
     1441 tggccgtcat tgccacacag gaccgccagc atgtccggca tgctgtcccg gcgctggaaa
     1501 aggggtatca ccttctgctc gaaaaacccg tctcgcccga ccttcaggaa tgtctttctt
     1561 tgcgcggcgc cgctcacaaa taccagcgca tcgttgcagt ggctcatgtc ctgcgctata
     1621 cgcagatatt ttccactgtc aagcagctgc tggacgccaa tgctatcggc cgcctgattc
     1681 atatggatct gattgagaat gtggcatact ggcacttcgc tcacagctat gtccgtggga
     1741 actggcgccg ggccggggag gcaagcccca tgatccttgc aaaaagctgt catgatctgg
     1801 atctgctgcg gtggctggcc ggagacacct gcatttcact ttcctcggta ggcgggttat
     1861 ctgttttctt tgcacagaac gcgccgaaag gcgcagcccg gcgctgtaca gagtgccgtg
     1921 tccgcggcga ttgcccctat aatgccgtgc agatctatat ggacaacgaa aagacaggca
     1981 ttctgcacgg caatacagat tggccctgct cggtgctgga cgcgcac
//
LOCUS       DAWBSB010000306         1887 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_8947_length_1887_cov_2.052948, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000306 DAWBSB010000000
VERSION     DAWBSB010000306.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8947_length_1887_cov_2.052948"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          405 a          537 c          479 g          466 t
ORIGIN      
        1 tctgcatggt gagcgtttct ttcgtgatat caaagaacgg ctgcttgatg acgtttccgt
       61 tttcatctgt ttccttccag cggtaacggt ccggattgcg gtaacttttt ttcgtatcct
      121 cgaactgata ggctgtgtcc tcgcccacca gcacgggaat cttgttgcgt cccagcgact
      181 ggcctgtcag ataactgccg ctgacaagct cgatccccat cggctcgatc gcatctacat
      241 ccatgcccac agcgttgtac atatacatgg aatagcggtc ttttttgccc gccaccacgt
      301 tcgcgttcat actttgaaaa ttgtacaacg gcgtggccgc cacaacattt tccatttcct
      361 tgaactgtgc gaccatttta tcatccagcg caggcgtatc cggattttcc gaatagctga
      421 acacctgaat ctgcgtcaga tcgctccaag tagacagcat ctcgtccgtg cgccggttgg
      481 tggcgatgcc gagggagatc attacgacaa tggcgcaggt tcccacgaca acaccgatca
      541 cggtgagtgc tgtacggcct ttgcggcggc gaaggttatc cgtgctgagc ttcagcaaat
      601 cagatatctt catcgtcatc ctccatgagc tgtgccgcgc ggcgtttcgc ctttttcttc
      661 ttcaaaacaa cgagaaccac gaccacagcc gccacaacgc ccacgcccac aacgatccac
      721 acccatacgg gcacgcctgt gggctccggc tccatcggga ccatagtgtc cataccgccg
      781 cccatcgtat cgtcgccgcc catctgcatc tcctctacgg tcatggagaa ctcttttgtg
      841 agcgtttttg cgttgccttt ctcgtcctca taagtgacgg tcaaaaggcc gttcagcgtg
      901 cctgcattgg cagtttgaat ggtaaagtct gcagagcttt cagttcctgc agcaatattg
      961 ccgatgaatt ggttgccctc ggtggttgtg attccatcgc ccgtgatctc ggccgaaaca
     1021 ttataaatga tgcctttgcc cttattcaca aaagaaacag aggcatagcc ttcctcgccc
     1081 atagaaagat actccgggaa atccgtgcgg gatatctcga agcgttcggg ctgtacaatg
     1141 ggtaccgtaa caggcatctg tgctgtgagc gcagcgctgt cagacgcgga atttcccgaa
     1201 acgtttactg taatattata gctgccgcca tttgccacag cgctggcatt cagttgaaac
     1261 gtcacctgtg tggaagcgcc cggtttcatg ttgcccacaa aaatattggt gctgccgctg
     1321 gcaacggtga tctgctcggg cagcgcaagg ttgacagcca cattttcggc ccccacggta
     1381 ccgccggaag ccagcacatc gacggtcagc gcgaaatcgc tgcccgcgtt gatgctgccg
     1441 ccgccatagc tggcggaggt gacgaccagc gccgaggcct tggcgccctc ggtggaagaa
     1501 cccacgcact ggctgatggg ctgcgacata gatttgagtt ggcgtgtatt gtcattgtac
     1561 gccaaatcga aagaaagctt attatccccg cccaagtaag taatgtcgtt caggataatc
     1621 gcatattgaa gacctgctgc accgacttta tcggcagcga atgtcgtcgt cgtgatatct
     1681 cccagcgaag gggatgcgaa cgatgctgtc gatgtaattt taatattcgc agccagtgcg
     1741 cgtccgtctg ccagtgtcaa ctgatcagcg gaggtaatac gcgggtcaac aaccgcaatg
     1801 ataatacggc atttctgccc ttcttcaatg cgctggagct ctgctcctgc agcatccgtg
     1861 actgtataag ccgcaatgta cgggtcg
//
LOCUS       DAWBSB010000307         1812 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_9322_length_1812_cov_2.602732, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000307 DAWBSB010000000
VERSION     DAWBSB010000307.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9322_length_1812_cov_2.602732"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          309 a          647 c          466 g          390 t
ORIGIN      
        1 aagcaggtgg tttgttcatt ttattcgtgc atgccgcaaa atcatttcgg ctgctccgtc
       61 gcagcctctg ccgcggttcc cttctccggc ttctcctccg cctgtgcggc ggcttcggcc
      121 tgctccgtcg cttcctccgc ggcagccgcc tgcgcggcgt ccgcggcctc ctccaccgcc
      181 tgcgcgccgg cggccacggt ttcctttacg cgctccagca gatcctccgc gccgtcgcgc
      241 agacggcccg caacggcagg agcctcctca gccacatatc cggcaaacgc cgagccagcg
      301 tccgccaccg cgtcgcccgc ttcatacagc gcgtctccgg cgtcgtgcag cgcccctttc
      361 agctccacgg tatccacgcc cgctttctcg gcagtgcctg tcaccacttc ctttaccttt
      421 gcgcccgcgt cgctcagcac gccgcccgcc gcgcgggcaa tatcctccag cacgctgccc
      481 gcgggtacgt ccggcggctc ggccccggcg gctgccgccg cagcgtcggc cgctttgttt
      541 tcctcatatt tttgggccac cttcaatgcg gcgaaagcgg cgcccgccgt tacgccaagg
      601 cccagcaagc cttttaaaaa tcccataact ctacgctcct ttacagcaaa atgggcaacc
      661 gcccgccctg cgtcaccgcc cggcgggctt ccttgctttc attatagatt ccccgcgccg
      721 gtttgccaag catttcgcgg catgtcacaa aaagtttgca attcgttccc ggtttggccg
      781 caaatgcgcc gtttcgcggc ggcttatgcc tgcgtgcgct cgatctcccg gatgcgcgca
      841 aagccttcgt gtaccatacc ggctttgtcc ggcgtcatgc cgtcgcacca gtcgctgtcc
      901 acaaatgcca gcgccatacg cccggccacc gcgggggtca cgactttttc gcccatggcc
      961 atcacgttcg cgtttgtgat gcgccgcagc tcggcggcag tatattcgct ttcacaggcc
     1021 acggcgtata cgccgcgaaa cttgccggcc gtcatgcatg cgccggcgcc gcttccgcac
     1081 atcacaaaac cgcgctcata cgcgccgctc tgaatggcgc gcgccaaaag ctcgcccgcc
     1141 tcgtaatagc gcacggtctc atcctcccgc tgctggccaa cgtccgtaac gtcgtggccc
     1201 gcctcccgca gcacctgctt gacatgctct ttcaaacgga atcccgacgc atcggatgca
     1261 atggcgattt tcatacaaaa actcctttcg acggtgtgcc gccgtttgtt ttcagcaatt
     1321 tcgcacagcc gtgccgcttt ttatcttatc ttaaaaaacc gcatgatgcg gtagccgatg
     1381 tagcggaaca catccgccgc cagccacagc tcgatgagcg cgtaatgggg cagattgcgc
     1441 caccggaatt tcggcttttt cacttttggg atggcccgga acgcctcccg cgcgcctttg
     1501 atgtacggcg tccagaatcc ccgcagcccg aacaccagaa ttttgagcag atagcccagc
     1561 gcaagcggga taaaattcag aaggagcatg cccagcggca tatttttgta gggcagcaga
     1621 atgctgttgc gcccgctctg cacgcttttg aattcgttgt accgcactgc gcccgtcgtc
     1681 gcgccgcaga tgtggtagca cttggccgcc gggcaataca ggtttttata acccagactg
     1741 tttgcccgcc agctgatgtc cacatcctcg aaataagcaa aaaacagttc gtcgaacaga
     1801 cctatctcat cc
//
LOCUS       DAWBSB010000308         1782 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_9489_length_1782_cov_2.116966, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000308 DAWBSB010000000
VERSION     DAWBSB010000308.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9489_length_1782_cov_2.116966"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          344 a          532 c          514 g          392 t
ORIGIN      
        1 cctccaaggc ggtgcttgcc accataggcc tttactatgc cgtgagcctt tggaacgcct
       61 atttcattcc gttcatctat ctgaacgacc ccgatctgct ctctttgcag ctcatcctgc
      121 acgagctgct gtcggccggt tcgtccaaca atgccaccgt gggcgtgggc gacgtgcttg
      181 tcgtggaaga atcgctgaaa tatgctactg ttctggtgtc catcgcgccc atcatgtgcg
      241 tgtatccgtt cctgcaaaaa tactttgtca aaggcgtcat gatcggcgct gttaagggct
      301 gaccgcccca aggaggaaat ttaccatgga acctgtccgt tctttttgca aggattctct
      361 tgccgtccgc atctatgccg accgcaccgc aatgggcgcg gcggccgcga gggacggggc
      421 tgctctgctg cgccgtctgc tggcgcagca ggacaccgtg cgcgccgtat ttgccgccgc
      481 tccttctcag aatgaatttc tggaagcact gtgcgccgcg ccggatatcg actggaaacg
      541 cgttaccgca ttccacatgg atgaatatgt gggcctggat gcagcggcgc cccaaggctt
      601 cggcaacttt ttgcgcgccc gcgtgttcga ccgtttgccc tttggccaag tatgctatct
      661 gaacggcaac gcgcccgacg ttgaggccga atgcagccgc tacgccgggc tgctggcaca
      721 ggcgccaatc gacattgtat tcatgggcat cggtgaaaac ggccatatcg ctttcaacga
      781 cccgcctgtg gcggaattcg acgatccggc cgcggtaaag gttgtcgccc tggacgaagt
      841 gtgccgcaac cagcaggtac acgacggatg ctttgctgat atcggccagg tgcccaccca
      901 cgccttgaca ctgacagtac ccacgctggc cggcgcaaag catcatctgt gcatcgttcc
      961 cgcgcccacc aaagcaaaag ctgtccgcga cactctggaa gggcctgtct ccaccgcgtg
     1021 cccggcatcc atccttcgca cctgccctgg tgcggtgctt tatctggagc gcgagagcgc
     1081 ccagcttctg acggaggtat aagatcatga acctgttttt catcccccag ccccagcggt
     1141 gcatctgtct gaccggaagc gcggacattg ggcgggaaac gcctgttgtg ctctgcatgc
     1201 cgcagccgga cgagcggctg gtgctggctg ccgggaaact gtttgaacat gtgcgcacgg
     1261 aacaggggcc tttcgcgctg tattcggaaa acagctttgc ccccgagtgt cccgtacggc
     1321 gtccggaggg atacttactg aatgtgcgcg gccccgccgt tcaacttgcc gctcatgatg
     1381 cgccggggtt gttttacggt atacagacgc tgcgccagta tctggaaatg ccggagcatc
     1441 ccgcgatgga gatcaacgat tggcctgaac tggcgatgcg cagcgattat ctggatatgc
     1501 gcggcctgtt tccaaagttc gagaatcttt tgaaatatgt ggaggaaatg gcccggtata
     1561 agctgaacac gctggtgatc gaatacgaag ataagctgcc ccgcagccgg aaggaattct
     1621 gtcatccgct ggatgctctg acgccggagc agcatgcgct gcttttgaaa acggcccacg
     1681 accattttat cgatgtgatc ccgctgcagc aaagcttcgg ccatttggag tatgcgctcc
     1741 agatgccgga gtaccagcat ctgcgcgaac taccggaggc tc
//
LOCUS       DAWBSB010000309         1741 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_9721_length_1741_cov_2.029063, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000309 DAWBSB010000000
VERSION     DAWBSB010000309.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9721_length_1741_cov_2.029063"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          440 a          540 c          443 g          318 t
ORIGIN      
        1 ccgtccgcaa aacctctgga aaatcaaatg gctgaaaggt aaggttcgac agatgcatgc
       61 cgcccaggtt ggagaccagc atcagtgcca tagagagtaa aaagaggcct gcggccagac
      121 cgagcacacg tacgatgctt tccagcccgc accgcagcgc ataaaacacc acaagaagaa
      181 acagcccgta taccaacagc tgcggcatcg gctcgtcgct cacatagcga aaaaatgcct
      241 cggaacgcac catcgctgcc gctcccccgg cggcgaacag caccgccgaa agcgtgagca
      301 gcgccttgga cccaaacgta acgccgccct tcagcgtggt gaaggcttcg cgccgcagca
      361 accccagcac gggcagaagc agtatgctga ctacagccgt gttgaggaca gcggccaaaa
      421 tcgcaatctg cgcgcccgcc gagcctccgg aggtaaatgg gcgcacagcc atatccaccg
      481 ccaatgagag gaacaccagt gtacagagct gttttgcgct cactgttgta tgctcgttca
      541 ttgcgtctcc cccgtttcct ttccgggcag ctggtcaatg gaaaaatctg cccgcgccag
      601 cgtatgccag ttgcgccgca gcaggccgtc cctccaaaat gcactgctgc ccggcgcgat
      661 gggcgctgta tacgggatac cgaaagaatt cataccgcac atccccagca gcatacaaaa
      721 cagcagcgta acgataccca aaggccccaa gaggccgccg gccaatatat acaggatgcg
      781 cagcactgtg atcggctcgt acagcgatgg cactacaaag gtgcaaatcg ctgtcattgc
      841 ggccacgatg accacaggtg tgcccacaat gcctgcgctg acggcagcat cccctacgat
      901 gagcgccgcc acaaggctca ccgaatggcc gatgggcttg ggcagacgca gccctgcctc
      961 ccgcacgatc tccagcagaa aattgacaaa cagtgcttcc ataaacagcg gcagcggcgt
     1021 ggcaagctcc gcagacgcaa ctttgtacag cagttccggc ggcagcagct ccggcgtgaa
     1081 attcgcaacg gacacgaaca cgcccggcag catcaccgcg atgaggaacg cagagtattt
     1141 cagaatgcgg ataagcgatg caaagtatgc tttttcggaa tagtcgtcca tgctctgaaa
     1201 attctcattg aaaaaataag gcacgatcat agcgaaaggg cttccattcg ccatgataat
     1261 gattttcccc tcgcagattt ttgcgcaggc agtatccggc cgttccgtgt atcctaccga
     1321 ttgaaaaaat gaaaatcggc ttttctgcaa aaacgccgcc agataaccgg tatcgaacaa
     1381 aaacggcagc ttaaccgatt ccagcctgct cttcacagtg gaaagcatct gcttcgggac
     1441 aagctgtgtg ttgtataaaa gcgcaatttc cgttttggta tgttccccca ctgtcatcgt
     1501 ttccaccatc agctcaccgc tgcgtatcag acgccgcatc aagctgatgt tcacacgaag
     1561 cggttctgta aagccttccc gcgagccacg gacattccct tccccgcttg gctcctgcac
     1621 cgagcgggac tgcatgctct gtgtagaaag aaccagcccc cgtgctacgc cgtcgatcag
     1681 aatgacgctg gtgcccgccg tcagctgcgc acgcgcatct tccacacaga caacgctttt
     1741 g
//
LOCUS       DAWBSB010000310         1734 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_9779_length_1734_cov_1.474687, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000310 DAWBSB010000000
VERSION     DAWBSB010000310.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9779_length_1734_cov_1.474687"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1190..1199
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          384 a          471 c          421 g          448 t
ORIGIN      
        1 ttcctgcgca ggctttccat cgttttgaca tttgctgcaa atgcagcaaa tattttttct
       61 ttcagcttct tcacctactt tgtctttttt catcacaagg ggtttggggc ccgccccaaa
      121 atgcgcgtgg atatccacgc gctatgctag ccaaaccagt cgctgcgcgg actttatgcc
      181 gcttgtgttc agcatgcata ccgatgttcg tcctccttga ttttcgcaag gccgtccagc
      241 gtggtgcaga tgagcttcag cttttccgct gtcccgatct cgcccgccat cgcggccgtg
      301 agctcgccgc cacacaccac cagcatccgg caccggcgca aaagctgctg gctgatctgg
      361 ctcatggccc gctgttcttc gggcacgtcc gcgctcagat actgcggcaa gctcagcttt
      421 gggcagatgg gtacatatcc caactcgtat accttgtggc agtatctgcg cagaaggcgc
      481 ggtgctgcgt cctgtgccgc acagatgtat gcaaatgctt tttccatttt gatttcaaat
      541 cctttcatct aaatgtatgt tcccggtaca gccgggatct tgtatggatt gacgtgccgc
      601 ctctttcttc ccattctcaa aagtggtata ttgtagatac aacagcaccc gacgcagaac
      661 aggaggcgct atatgaaaga gcttaaaaga aaacattttc gctttatcga agttgcagaa
      721 aatgtttatg ccgccattgc cccggccgag gaactggata ttaccgacgc cggccttatg
      781 gacaactttt ccaactccgg actcgtattg caaggcggcg ggctggtttg cgatacctgt
      841 ttcgacttgc cgcatgcaaa cgagttaaaa tctttctgtg aagacgcaat gggccatgcg
      901 ccggagtatg ttgtaaacac acacggccat tgggatcact tctggggcaa ccaggtgttt
      961 tcgcaggctc gggtcatggg ccaccgcgac atgctgaaag actgcggcaa tgataaatcc
     1021 aaagtacctg ttttccaaat gctgcacaat tccaaaggaa tcgaacacat tttaagcggg
     1081 ctgatgagaa cgcaatttaa gccatatctg cccaaaggac aaccgttcca catgattgtc
     1141 cggcaaagtg aacacgattt tgatttaaca gatgttgttc cttcccctcn nnnnnnnnng
     1201 tttgccggcg atatctttgc cgattgcagc cttccaatgg atttggcttc tgcccatcgc
     1261 tggctggagg ttttggacta tctgttggac gaactccagc ctgtgtggat cgtaccgggg
     1321 cacggagaac tctatgacct tgcccgcgct caaaatcaac gtgaatactt ccgcgcgctg
     1381 gtcggccaat tcgagcgttt ctacacagac acgatctctg aaaaagaact gcttgataaa
     1441 atggaactaa cgccatatct tgaccaccgg cccagacttg gttgggtcat ggccgtaaaa
     1501 acctttttca aagaaagacg aaaagcgtca aagtccggta aatgatttga agggcaggaa
     1561 agcaggcatc caatgtggat ggctgccctc tctgtgttca tctcttttgg gcatccaatt
     1621 ggatgccctg ttctctgttg gacgcttccg ccgtccgttc gtcaccggct gcaagtgccg
     1681 tgcgcacacg gtcgctgccc agcctgtagt acgcgtccgt cacttcaacg ccca
//
LOCUS       DAWBSB010000311         1703 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_9972_length_1703_cov_2.608010, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000311 DAWBSB010000000
VERSION     DAWBSB010000311.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9972_length_1703_cov_2.608010"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          312 a          526 c          457 g          408 t
ORIGIN      
        1 cccacgtcgc gtgtgatgga gggattctcg cttttgcggg tgatcttgtc gatctcgtcg
       61 atgtaaatga tgccccgctc tgcccgctca atatcgaaat ccgcagcctg aatgagcttg
      121 agcagaatgt tttccacatc ttcgcccaca tagcctgcct cggtgagcgt agtcgcgtcc
      181 gcgatggcaa agggcacatt gagcatgcgc gccagcgtct gtgccagcag cgttttaccc
      241 acgccggagg ggccaagcag cagaacattg ctcttttgca ggtccacatc cgtcttttcc
      301 atgttcagga tacgcttata gtggttgtac acagaaacag ccagtaccac cttggcattg
      361 tcctgcccga tgacatactg gtccagccct tcctttatct ccgccggcgt gaggatattg
      421 atatcctgcg gcgcggcatg ggccggggca gccttgccgc ggcgcggggc ctgagccgga
      481 gccttgcctt cctcatccag cagcgagtgg cacagctcga tgcactcgtt gcagatgttc
      541 acacccggcc caatgatcat ccgctccact tcgtcctggc ttttgccgca gaagttgcag
      601 atgagatttt tgtctttgct gttgtcttta tttgccatat aacaccttta tcgttgtgag
      661 atcacgtcgt caataaggcc gtaagccttt gcttcctcgg ccgtcatata attgtcgcgc
      721 tccgtgtcct gctcgatgcg ttccagcggc tggccggtgc aagcggagag gatgccgttc
      781 atcttattgc gcgtgcgcac catatggtcc gcgtggatct tgatatccgt cgtctgcccc
      841 tgcagcccca cctgtccgcc gatcagcggc tggtggatga gtatttcgct gttgggcagc
      901 gcgaaacgtt tgccctttgc gcccgccgca agcagtacgg aagccatgga ggccgccata
      961 cccacacaga tggtggaaac gtcgcatttg atataccgca tggtgtcata aatcgccatg
     1021 ccgtccgtga tggacccgcc cgggctgttg atgtagaaat tgatatcctt ctccgggtcc
     1081 tgaccttcca gatacagcag ctgcgccacc acaaggctgg ccgtcgtgct gttcacatcc
     1141 tcgctgagca tgatgatgcg gtcgttcaac aggcgggaaa agatatcgta agagcgttcg
     1201 ccgcgcgcgg tctgctctac aacgtaagga accaaactca taaattagcc tccctgtttt
     1261 acagcggttc agtctttatg ggaaatcgca tggccgcggc agcccgctgt acccggccgc
     1321 gcggcccgcc gcaggcgggc cgtaaaaccg gccgccgcag cagggtaaca gatgaccgat
     1381 acgcgcataa aatgcgggta tcggtcacgc gtcgtcacta tccgacaaaa aggcccgctt
     1441 tactccgcct tctcgtcctt ctggggcgcc acattctcaa cggtgacgtc cgcgttctgc
     1501 ttgatgaagt caatggcctt gttcacggca aggtccttct tcacttcggc ctcgggcacg
     1561 atgccgcgca ccttctccac ttccatcttg taggcgtcgg caatgcgctt gacttcgttc
     1621 tcgaagtcct cctcgctggc cgcgatgcct tctgctgcag ccacagcctc cagcgccaga
     1681 cggatcttca cctgcttctc agc
//
LOCUS       DAWBSB010000312         1593 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_10730_length_1593_cov_3.037061, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000312 DAWBSB010000000
VERSION     DAWBSB010000312.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10730_length_1593_cov_3.037061"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          304 a          545 c          436 g          308 t
ORIGIN      
        1 gctctccagc tgtcgggcga taaaagcgct gggacagtgg atatggtcga accggttgta
       61 aaacaccttg tagaaccacc agtacaggaa atcattcacg cgcctgtttt tgcccaaaaa
      121 aatgctggag gtgatattct ccggctgcac gtgaaaggcc gccactgtgg gaatgcccat
      181 ctggcgggcc agttcctctc ccctgcggca aaagcggaac ggcatataaa agtgcacgat
      241 atcggcgtcc ttgaaggccg tgtaatatgc ctcttcatcg ggcttcgcaa agcacatgcc
      301 ctggctttcg atgagcttct ggaacaccgg gatacggtgc accggagcgc atatcttgcc
      361 ctcgcacggc gcgccgcccg caagaatgac aacctcgtgc ccgcgcctgc gcagctggcc
      421 cgcaaagcgc cgcgccgtca cagtggtgcc gttgtttgaa tcgtcgaact ggtcgatcac
      481 caaaacaatt ttcattcaga gcctccattg cccggggcgt tttcacgctc caatatctgc
      541 tgcaaaagca gtgcggcgcg cgctttgcgc cgctttgttt cctccggcgc ggcactccat
      601 acgccgtccc tgtgctccag cacatccccc tgcgccgcac cgggcggcag gtctgccgca
      661 ggcacgaacg cactgccgcc cgcgtcatcc accagacgcg ccgttccgtt ttccaacgcg
      721 tccacagaat aatacatcac gccgcctcct gtctgcttac ggccatctgc aagacccccg
      781 ccttgtctac atacgcaatg attgtaccct ccgtatcggt acgatagacc tgcgccccca
      841 catttgcaaa cgcggccagc gcctcgctgt ggggatgtcc gtaggaattg cccgctccgc
      901 aggagaccac aacggcctgc gggcgcaccg catccagaaa tgcctgggta ttggacgtgg
      961 acgagccgtg atgcgccgct ttgaaaacat ccgcatgtac atccgcgccg cttgccagca
     1021 ccgcgtcctc cacggccttt tcgccgtcgc cgctggacag gcagcgcagg cccggcgcct
     1081 caaacagcgt caccagagag atatcgttga gattttccgc ggccacaccg tcgcccagca
     1141 ccgtgagcgt cccttctccc agcgggaaca catctcccgc gcgggccgtc acagtgggga
     1201 tctgcttttc ccggatggcg tccagcagat tcaggaaggt ggatgtagtg ggcatcggcg
     1261 cttttgcaaa atccggcagc accgccctgt ccacagggaa cgcatccagc acggcctgca
     1321 tgccgcctat atggtccgca tggggatgcg tcatcaccag caggtccagc tttgcaacgc
     1381 ctgcggcctg caagtacgcc accagcccgt ccgccgcttc ccgctcgcct gcgtcgatga
     1441 gtgcgaatgc gccgttctgt tccagcagaa cagcgtctcc ctgccccacg tcgataaaat
     1501 gtacttttgt agccgcgccc gccgcttcct ccggcagatg cggcacgggc gctgctacgc
     1561 cgaaccagct gtacagctgc tgccaggtgg gca
//
LOCUS       DAWBSB010000313         1571 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_10900_length_1571_cov_1.998681, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000313 DAWBSB010000000
VERSION     DAWBSB010000313.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10900_length_1571_cov_1.998681"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          366 a          418 c          474 g          313 t
ORIGIN      
        1 gtgtccagcc ggctgctgca cagcaactgt catgagccga cggcagacga gatcgcggag
       61 gagttggaca tgcccgtgga taaggtgcgg gagatcatgc gcgtagcgca ggagccggtg
      121 agcctggaga cgcccatcgg cgaggaagag gacagccact tgggtgattt tattcccgac
      181 gaggatgcgc ccgtccccgc cgaggcggcc agccacacgc ttttgaagga gcagctctcc
      241 ggcgtgctga aaagccttac gccccgggag gaaaaggtgc tgcgcctgcg cttcggcctg
      301 gaggacggcc gtccccgtac gctggaggaa gtgggcaagg agttcaacgt cacgcgcgaa
      361 cgcatccgtc agatcgaggc caaggcgctg cgtaagctgc gccaccccag ccgttccaag
      421 aagctgcgcg atttcctgga ctgatgcggc ccccgccttt gccgctgcct caatggcagg
      481 accgggcgta catatagaat ggatagagtg gactttgaac agcgcggccc ggcaaaaccg
      541 gagccgcgct gtttttgcac cgccgctgcg gcacaggaaa cgcgcttgac aaaagcgccc
      601 ggttgcggta tcctttaaat acagtaaaac ggtatagttc aaggagcggt ttatgcatat
      661 cacacaggaa tccgattacg cggtgcgcat cgtctactgc cttgcaaagt gcggcacgcg
      721 gcgcgacgcg cgcggcatca gcgaagaaat gtgcgtgacg ctgcgctttt ccctgaagat
      781 attgggcaag ctggtgagca gcggcattgt ggaaagctac aagggcaacc gcggcggcta
      841 tgagctggca cgccccgcca gcgagatcac actcaaggac gtcattgacg cggtggaagg
      901 gccgtatagg ctttcccgct gtgtgggcga gggcggcgcg ggggagtgca accgcggcgc
      961 gtccggctgc tgtacattcc aaaaggtgtt tcacgatatc agcaaaagca tcaacgagca
     1021 gctcgctgcc gtcaacttcc agatgctgtt ggaggaaccg gccggcaagt aagaagcgaa
     1081 aatcacacac ggcgccgtgc gtgccaagtg tggttttttc ttttcctctt ttttccgggt
     1141 tccccttgac ggcttgtaaa aaccgtatta taaagcttaa atgtatcata atggcttggt
     1201 atgtctctga gcgcaaaact gttacaaaat ttttgtgcaa gttgctgaaa atcggaggag
     1261 tgccaaagcc gttttgcgtc attttttgca aggcttaata tagatgaatt aaggagtggt
     1321 cgcaacttat ggtgaaagtc aagcccgtac aacttggcaa aaccgaacgc atgagcttct
     1381 cgcgcatcga cgaagtcatc gacatgccga acctgatcga ggtgcagaag aactcatacc
     1441 agtggtttct caaagagggc ctgaaagagg tcttccggga catttcgacg atcgaagact
     1501 acaccggcaa cctggtactc gactttatcg actaccggct ggattccgag cccaagtact
     1561 ccatcaagga g
//
LOCUS       DAWBSB010000314         1526 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 NODE_11235_length_1526_cov_2.694086, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000314 DAWBSB010000000
VERSION     DAWBSB010000314.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11235_length_1526_cov_2.694086"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          366 a          402 c          398 g          360 t
ORIGIN      
        1 ccatgagcat cgctgacgca aacgtcgtgc tgaacaccgc agttgccaaa gagctgaaag
       61 gctatgccga cgagctggag aatgcggagg atttcgacac cgccgttatc aaactggtga
      121 agcgcaccat ccgcgaccac aagcgcatca tcttcaacgg caacggttac agccgtgaat
      181 gggaacaaga ggcggaaaag cgcggcctgt gcaacctgaa atccaccgta gacgcgctgc
      241 cctgtgtgtt ggaacgaaaa aatatcgaac tgtttgagga attcggcatt ttcagcgagg
      301 ctgaggtgcg cgcccgttat gaagtgagcc tggaaaacta caccaaagcg cttcacattg
      361 aagcgctgac catgttggat atggccaaaa aacagattgt cccggcggta gccgcctaca
      421 tgggcgacct tgccgatacc gcctgcagca aaaaggcact gtcctctgcc ctttccaccc
      481 gcgctgagga aacgctgctg gccaagctct cagccggggc cgacatgctg gtggaaaaaa
      541 tcgacgagct gcaggccgcc gtggacggcg cttcccgcgg cgacagcctg caggaggccg
      601 cctgctacaa agacagggtg ctgccgaaga tggccgagct gcgcgcggtg gctgatgcgc
      661 tggagcttgc cacggggaaa gactactggc ccttccccac ttacgcagat cttctgttcg
      721 gcgtaaactg aacccgtacc ggttccgtcc ggtacggggc acggaacctt ttccttattc
      781 tcctatactg gcaaagggcg ttgccgcgca agcggcagcg ccttttgtca attttggggc
      841 agatatacaa ttatgtatgc agttctttcc aacaggctta acataaagta tgtctcccat
      901 gggaaagaag ctcttttttc caccacatag tgcgcaccgc gcttttctgg gataaatcat
      961 ccgttttccc tcttgctcat ccacgcagag aacatctccc gcttgaagat ttttttgttt
     1021 gcgataacct gcaggcagta cgatcctccc caacgcatcc atctgtcgct ccatcgtttt
     1081 cactccgttt ctttttattt cagcatcatt cttttttgga attatatcaa gttgttttct
     1141 ccttataatt ctgttaaaga atcaaaaaga ggagagcggc agatgcggga acgtgtggca
     1201 ccactgggtg ataaaaatat tgtcggcgcg accgtcaccc ggctccgcct tgaacagggt
     1261 attacacaaa aagcgttgat ggctaaaatg cagacctttg gtgctgatat cagctattcc
     1321 agtttatcaa agttggaggg ccagacccgt tcggtttctg acaaagaggt ttatgcgctc
     1381 gcccgaagtc ttgaagtaga gatcagcgca ttgttttcag taaaataaaa gccgtcccat
     1441 gagatatggg gcggctttta taatctgttt tccgggctta aaacgtgccc gggatgatgc
     1501 tgcggctctc atcgaaagca atgggt
//
LOCUS       DAWBSB010000315         1256 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 k99_9063_length_1256_cov_6.0000, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000315 DAWBSB010000000
VERSION     DAWBSB010000315.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1256)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_9063_length_1256_cov_6.0000"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          246 a          350 c          436 g          224 t
ORIGIN      
        1 agcgaaatgg agccgaacgg cgacaagcgc cggatattcc gaaggaggtt tctatgaagg
       61 atctttttga aggcgtgtat ctcaagcacc agaaaaacgg cgagagcatc tgtttcattg
      121 ccgggcgcgc gggggaaacc cggttcgtcc aggtgatcac caacgggacc gtccggcagt
      181 acgacggggc gcagggcttc tgctttgggg aggagggcgt ggcggccgag ctgcccggcg
      241 tgcagggcgc ggtgcgctac ggcccgctgt cgccgctcag gacggacatc atggggccgt
      301 tccgctttct gccgatgcag tgcagccaca gcgtatacag catggcgcac acgctggcgg
      361 gcgggttcac ggtggacggc cgattcatcg acctgacggg cgggaccgga tacattgagg
      421 gcgaccgggg cagcgccttt ccacgggact acctgtgggt acagtgcaac gattttgcgc
      481 agccgtgcag cgtgatggcc tctgtcgcgc atatcccgtt ctgcggacgc tcctttacag
      541 ggtgcatctg tgccgttgtg taccgcggcc gtgaataccg gctggccacc taccggggcg
      601 tgcgcatcgt ggaggcgtcg tcccgcaggc ttgtgctgcg gcagggcagg tatctgctgg
      661 aagcgtcctt cgcggcgcgg gaggcacatc cgctgcgcgc gccccgaaca gacggcatga
      721 cgcggaccat ccgtgagtgc aaccgcgccc gggcacggtt ccgcttttgg gagggcggcg
      781 gccttgtgtt cgacctgtgc agcgaaaacg ccagcctcga gtgcgaggag gctgtgccgg
      841 acggcgcaaa aaggcgcggc gcactgctgt aaaacgccgc ttgtgggaaa aaaggaaggc
      901 ccccgcgaaa gcgggggctt ttggccggga ataaaaggtt tatcacatgg aaaggaaagg
      961 cccgaaataa aaagaagtct gctcccgtcc cgtaaggagc agactgcatt tggtgtgctg
     1021 caaatttgaa aatggctcaa tctcaaagcc ttttgcgcct tgcgccgggg ccagagggaa
     1081 gccgcagcga aagctttgcc ggcccaaagc agccaggcgg tgtattaagt ggagtgaaag
     1141 caaaacgtat tgcgcgctta tagcgtgagg cttccggctt ccttgccggc gatggcgaag
     1201 gcgaacagcg cgctcttgtc caccgcagca aagtagccgg tgcgcgcata ttggtc
//
LOCUS       DAWBSB010000316         1043 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruthenibacterium lactatiformans isolate
            gSpreadC_ID_1654 k99_8075_length_1043_cov_4.0000, whole genome
            shotgun sequence.
ACCESSION   DAWBSB010000316 DAWBSB010000000
VERSION     DAWBSB010000316.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994786
            Sequence Read Archive: SRR3917697
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruthenibacterium lactatiformans (human gut metagenome)
  ORGANISM  Ruthenibacterium lactatiformans
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruthenibacterium.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Ruthenibacterium lactatiformans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_8075_length_1043_cov_4.0000"
                     /isolate="gSpreadC_ID_1654"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1550024"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          227 a          302 c          276 g          238 t
ORIGIN      
        1 cctgcgcacg tacggcacag gcaggcttat cgtccttgct cctcagcgga tatggggctt
       61 aacaggcttt ttcgccagca gcaggccgac gacactgaag acgatcgccg tgacggagac
      121 cacggtgccg acgatgccaa gcacaaaacc tacgatacga aatgcgctgc ctcttttcat
      181 gtgcgccaac tcctttccgc aggatgggcg gcgctgtgcc gcctatcggt gttgttgtta
      241 tcagtatact atatttttca cgcgcttgca agaaattcct gttttttgcg caaacagtcg
      301 catttgtgct ttttgtatat aaaatttgct aacattcatc attattttat ttatttcacc
      361 gtattcctgt gttataataa aaacagggaa agttttcctt caccgttttt gaaaggagat
      421 gttttgtatg tcacggcact atgtcatcac catcagccgc cagttcggaa cgggagggcg
      481 cgagatcgga aaagcgctgg gcgccgcctt gggggtgccc gtatatgacc gggagctgct
      541 ggaagaggcc gcccggacat actgcatccc cgtgagcgtg atggagcgca atgacgagac
      601 cgcgcccagc cgcgtgctgg aatcttttgc gctgagcgcc ccgtccctga gcatgctgac
      661 cggcgcggtg gattattcca ccatcgtcac ctcagacacc acctttgcct ggcagtccgc
      721 caccatccgc cggctggcca aagaaggtcc gtgcgttatc atcggccggt gcgcggacca
      781 tgtgctgcgc ggcacgccgg gtgtggtctc gctgttcctt tatgccgatt acgaggcccg
      841 cgtggaccac atcacccaga aataccagct ctcccgcggg gacgccacca ttcttatcga
      901 cgaaacagaa aaagagcgtg cccgctacta caagcgccac acagggcagg aattcgcccg
      961 catggaaaat tacgacctct gcctgaacac agccagcgtc aaccgcgaaa tggcgattct
     1021 gctgctgaaa gcctttgtcg aaa
//
